title: Examination of endothelial cell-induced epidermal regeneration in a mice-based chimney wound model.
abstract: As wound contraction in the cutaneous layer occurs rapidly in mice, mechanical means are typically used to deliberately expose the wound to properly investigate healing by secondary intention. Previously, silicon rings and splinting models were attempted to analyze histological recovery but prevention of surrounding epidermal cell migration and subsequent closure was minimal. Here, we developed an ideal chimney wound model to evaluate epidermal regeneration in murine under hESC-EC transplantation through histological analysis encompassing the three phases of regeneration: migration, proliferation, and remodeling. Human embryonic stem cell derived endothelial cells (hESC-EC) were transplanted due to possessing a well-known therapeutic effect in angiogenesis which also enhances epidermal repair to depict the process of regeneration. Following a standard 1 mm biopsy punch, a chimney manufactured by modifying a 1.7 mL microtube was simply inserted into the excisional wound to complete the modeling process. Under this model, the excisional wound remained fully exposed for 14 days and even after 4 weeks, only a thin transparent layer of epidermal tissue covered the wound site. This approach is able to more accurately depict epidermal repair in relation to histology while also being a user-friendly and cost-effective way to mimic human recovery in rodents and evaluate epithelial repair induced by a form of therapy.
authors.affiliation: Department of Stem Cell Biology, School of Medicine, Konkuk University, 120 Neungdong-Ro, Gwangjin-Gu, Seoul, 143-701, Korea.
authors.forename: Joseph
authors.initials: J
authors.lastname: Seo
authors.affiliation: Department of Stem Cell Biology, School of Medicine, Konkuk University, 120 Neungdong-Ro, Gwangjin-Gu, Seoul, 143-701, Korea.
authors.forename: Soon-Jung
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Department of Stem Cell Biology, School of Medicine, Konkuk University, 120 Neungdong-Ro, Gwangjin-Gu, Seoul, 143-701, Korea.
authors.forename: Jong-Jin
authors.initials: JJ
authors.lastname: Choi
authors.affiliation: Human and Environmental Toxicology Program, University of Science and Technology, Daejeon, Korea.
authors.forename: Sun-Woong
authors.initials: SW
authors.lastname: Kang
authors.affiliation: Stem Cell Research Laboratory, CHA Stem Cell Institute, CHA University, 605-21, Yoeksamsam 1-Dong, Gangnam-Gu, Seoul, 135-907, Korea.
authors.forename: Joa-Jin
authors.initials: JJ
authors.lastname: Lim
authors.affiliation: Stem Cell Research Laboratory, CHA Stem Cell Institute, CHA University, 605-21, Yoeksamsam 1-Dong, Gangnam-Gu, Seoul, 135-907, Korea.
authors.forename: Hye-Jin
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Department of Stem Cell Biology, School of Medicine, Konkuk University, 120 Neungdong-Ro, Gwangjin-Gu, Seoul, 143-701, Korea.
authors.forename: Jong-Soo
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, 135-710, Korea.
authors.forename: Heung-Mo
authors.initials: HM
authors.lastname: Yang
authors.affiliation: Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, 135-710, Korea.
authors.forename: Sung-Joo
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Mirae Cell Bio Inc./Jeju National University Stem Cell Research Center, Seoul, 143-854, Korea.|Faculty of Biotechnology, College of Applied Life Science, Jeju National University, Jeju, 690-756, Korea.
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Kim
authors.affiliation: Mirae Cell Bio Inc./Jeju National University Stem Cell Research Center, Seoul, 143-854, Korea.|Faculty of Biotechnology, College of Applied Life Science, Jeju National University, Jeju, 690-756, Korea.
authors.forename: Se-Pil
authors.initials: SP
authors.lastname: Park
authors.affiliation: Department of Medicine, School of Medicine, Konkuk University, 120 Neungdong-Ro, Gwangjin-gu, Seoul, 143-701, Korea.
authors.forename: Sung-Hwan
authors.initials: SH
authors.lastname: Moon
authors.affiliation: Department of Stem Cell Biology, School of Medicine, Konkuk University, 120 Neungdong-Ro, Gwangjin-Gu, Seoul, 143-701, Korea.
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Chung
mesh_terms: D000818:Animals; D024102:Collagen Type VIII / Q000378:metabolism; D003362:Cost-Benefit Analysis; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D000066449:Human Embryonic Stem Cells / Q000637:transplantation*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D012038:Regeneration / Q000502:physiology*; D033581:Stem Cell Transplantation / Q000379:methods*; D014945:Wound Healing / Q000502:physiology*; D014950:Wounds, Penetrating / Q000503:physiopathology* / Q000628:therapy
chemical_list: D024102:Collagen Type VIII