title: A Novel Regenerative Technique Combining Bone Morphogenetic Protein-2 With Fibroblast Growth Factor-2 for Circumferential Defects in Dog Incisors.
abstract: Periodontal regeneration of incisors is necessary for esthetic recovery. A novel regenerative method combining bone morphogenetic protein (BMP)-2 and fibroblast growth factor (FGF)-2 was developed. The purpose of this study is to evaluate periodontal healing, including root coverage, in circumferential defects of incisors.
  
  
  
  Fifty incisors in five beagles were used. After circumferential defects were surgically created, each group, consisting of ten recipient sites, received: 1) a double layer with FGF-2 (2 μg)/collagen as inner layer and BMP-2 (4 μg)/collagen as outer layer (FB-DL group); 2) collagen impregnated with both FGF-2 (2 μg) and BMP-2 (4 μg) (FB-M group); 3) BMP-2 (4 μg)/collagen (B group); 4) FGF-2 (4 μg)/collagen (F group); or 5) collagen (C group). Dogs were sacrificed 8 weeks post-surgery, and healing was evaluated histologically.
  
  
  
  The three groups treated with BMP-2 showed enhanced new bone formation compared with control and F groups (P < 0.05). Furthermore, connective tissue attachment with cementum regeneration in the FB-DL group was significantly greater than in FB-M and B groups (P <0.05). Ankylosis in the FB-DL group was significantly less than in FB-M and B groups (P <0.05). Gingival recession was inhibited significantly better in FB-DL and FB-M groups compared with control and B groups.
  
  
  
  These data support development of a double-layer method combining BMP-2 and FGF-2 as a therapeutic approach to periodontal regeneration at incisors with horizontal circumferential defects.
authors.affiliation: Department of Periodontology and Endodontology, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan.
authors.forename: Emiko
authors.initials: E
authors.lastname: Saito
authors.affiliation: Department of Crown and Bridge Prosthodontics, Hokkaido University Graduate School of Dental Medicine.
authors.forename: Akira
authors.initials: A
authors.lastname: Saito
authors.affiliation: Kato Dental Clinic, Mito, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kato
authors.affiliation: Shibukawa Dental Clinic, Asahikawa, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Shibukawa
authors.affiliation: Division of Clinical Education, Hokkaido University Graduate School of Dental Medicine.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Inoue
authors.affiliation: Department of Crown and Bridge Prosthodontics, Hokkaido University Graduate School of Dental Medicine.
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Yuge
authors.affiliation: Department of Crown and Bridge Prosthodontics, Hokkaido University Graduate School of Dental Medicine.
authors.forename: Toshinori
authors.initials: T
authors.lastname: Nakajima
authors.affiliation: Support Section for Education and Research, Hokkaido University Graduate School of Dental Medicine.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Takahashi
authors.affiliation: Department of Periodontology and Endodontology, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan.
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Kawanami
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2; D001861:Bone Regeneration; D003094:Collagen; D003739:Dental Cementum; D004285:Dogs; D004955:Esthetics, Dental; D016222:Fibroblast Growth Factor 2; D007180:Incisor; D012038:Regeneration
chemical_list: D055396:Bone Morphogenetic Protein 2; D016222:Fibroblast Growth Factor 2; D003094:Collagen
keywords: Bone morphogenetic protein-2; fibroblast growth factor-2; gingival recession; periodontal regeneration; reconstructive surgical procedure