title: Recent advances in the production of recombinant subunit vaccines in Pichia pastoris.
abstract: Recombinant protein subunit vaccines are formulated using defined protein antigens that can be produced in heterologous expression systems. The methylotrophic yeast Pichia pastoris has become an important host system for the production of recombinant subunit vaccines. Although many basic elements of P. pastoris expression system are now well developed, there is still room for further optimization of protein production. Codon bias, gene dosage, endoplasmic reticulum protein folding and culture condition are important considerations for improved production of recombinant vaccine antigens. Here we comment on current advances in the application of P. pastoris for the synthesis of recombinant subunit vaccines.
authors.affiliation: a Institute for Translational Medicine, Medical College of Qingdao University , Qingdao , China.
authors.forename: Man
authors.initials: M
authors.lastname: Wang
authors.affiliation: b State Key Laboratory of Virology , College of Life Sciences, Wuhan University , Wuhan , China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Jiang
authors.affiliation: b State Key Laboratory of Virology , College of Life Sciences, Wuhan University , Wuhan , China.
authors.forename: Yefu
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D003001:Cloning, Molecular; D003062:Codon / Q000737:chemistry / Q000276:immunology; D003470:Culture Media / Q000737:chemistry; D018628:Gene Dosage; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D000072235:Mating Factor / Q000235:genetics / Q000378:metabolism; D010843:Pichia / Q000235:genetics* / Q000378:metabolism; D015202:Protein Engineering / Q000379:methods*; D017510:Protein Folding; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D022223:Vaccines, Subunit; D058425:Vaccines, Virus-Like Particle / Q000096:biosynthesis* / Q000235:genetics; D014765:Viral Vaccines / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D000956:Antigens, Viral; D003062:Codon; D003470:Culture Media; D011994:Recombinant Proteins; D022223:Vaccines, Subunit; D058425:Vaccines, Virus-Like Particle; D014765:Viral Vaccines; D000072235:Mating Factor
keywords: Pichia pastoris; immunogenicity; protective efficacy; recombinant expression; subunit vaccine