title: High dietary fructose does not exacerbate the detrimental consequences of high fat diet on basilar artery function.
abstract: The objective of the study was to determine the effects of a high fat (HF) diet alone or with high fructose (HF/F) on functional and structural changes in the basilar arteries and cardiovascular health parameters in rats. Male Sprague Dawley rats were fed either a HF (30%) or HF/F (30/40%) diet for 12 weeks. The basilar artery was cannulated in a pressurized system (90 cm H2O) and vascular responses to KCl (30 - 120 mM), endothelin (10(-11) - 10(-7) M), acetylcholine (ACh) (10(-10) - 10(-4) M), diethylamine (DEA)-NONO-ate (10(-10) - 10(-4) M), and papaverine (10(-10) - 10(-4) M) were evaluated. Rats were also monitored for food intake, body weight, blood lipids, blood pressure, and heart rate. At death, asymmetrical dimethyl arginine level (ADMA) and leptin were assayed in serum. Although there was no significant difference in weight gain and food intake, HF and HF/F diets increased body fat composition and decreased the lean mass. HF/F diet accelerated the development of dyslipidemia. Although resting blood pressure remained unchanged, stress caused a significant elevation in blood pressure and a modest increase in heart rate in HF fed rats. Both HF and HF/F diet resulted in decreased response to endothelium-dependent and -independent relaxation, whereas increased basilar artery wall thickness was observed only in HF group. Serum leptin levels positively correlated with wall thickness. Moreover serum ADMA was increased and eNOS immunofluorescence was significantly decreased with both diets. These data suggest that the presence of high fructose in a HF diet does not exacerbate the detrimental consequences of a HF diet on basilar artery function.
authors.affiliation: Department of Pharmacology and Therapeutics, University of Florida, Gainesville, FL, USA.|North Florida/South Georgia Veterans Health System, Geriatric Research, Education, and Clinical Center, Gainesville, FL, USA.
authors.forename: H Z
authors.initials: HZ
authors.lastname: Toklu
authors.affiliation: Department of Biomedical Sciences, Florida State University, Tallahassee, FL, USA.
authors.forename: J
authors.initials: J
authors.lastname: Muller-Delp
authors.affiliation: Department of Pharmacology and Therapeutics, University of Florida, Gainesville, FL, USA.|North Florida/South Georgia Veterans Health System, Geriatric Research, Education, and Clinical Center, Gainesville, FL, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Sakaraya
authors.affiliation: North Florida/South Georgia Veterans Health System, Geriatric Research, Education, and Clinical Center, Gainesville, FL, USA.|School of Dentistry, Marmara University, Istanbul, Turkey.
authors.forename: S
authors.initials: S
authors.lastname: Oktay
authors.affiliation: Department of Pharmacology and Therapeutics, University of Florida, Gainesville, FL, USA.|North Florida/South Georgia Veterans Health System, Geriatric Research, Education, and Clinical Center, Gainesville, FL, USA.
authors.forename: N
authors.initials: N
authors.lastname: Kirichenko
authors.affiliation: Department of Pharmacology and Therapeutics, University of Florida, Gainesville, FL, USA.
authors.forename: M
authors.initials: M
authors.lastname: Matheny
authors.affiliation: Department of Aging and Geriatric Research, University of Florida, Gainesville, FL, USA.
authors.forename: C S
authors.initials: CS
authors.lastname: Carter
authors.affiliation: Department of Psychiatry, University of Florida, Gainesville, FL, USA.
authors.forename: D
authors.initials: D
authors.lastname: Morgan
authors.affiliation: Department of Pharmacology and Therapeutics, University of Florida, Gainesville, FL, USA.
authors.forename: K Y E
authors.initials: KY
authors.lastname: Strehler
authors.affiliation: Department of Pharmacology and Therapeutics, University of Florida, Gainesville, FL, USA.|North Florida/South Georgia Veterans Health System, Geriatric Research, Education, and Clinical Center, Gainesville, FL, USA.
authors.forename: N
authors.initials: N
authors.lastname: Tumer
authors.affiliation: Department of Pharmacology and Therapeutics, University of Florida, Gainesville, FL, USA. scarpace@ufl.edu.
authors.forename: P J
authors.initials: PJ
authors.lastname: Scarpace
mesh_terms: D000818:Animals; D001488:Basilar Artery / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D001786:Blood Glucose / Q000032:analysis; D001794:Blood Pressure / Q000187:drug effects; D059305:Diet, High-Fat; D005632:Fructose / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D006339:Heart Rate / Q000187:drug effects; D020738:Leptin / Q000097:blood; D008055:Lipids / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D014661:Vasoconstriction / Q000187:drug effects; D014664:Vasodilation / Q000187:drug effects
chemical_list: D001786:Blood Glucose; D020738:Leptin; D008055:Lipids; D005632:Fructose; D008315:Malondialdehyde; D005978:Glutathione