title: Late stage definitive endodermal differentiation can be defined by Daf1 expression.
abstract: Definitive endoderm (DE) gives rise to the respiratory apparatus and digestive tract. Sox17 and Cxcr4 are useful markers of the DE. Previously, we identified a novel DE marker, Decay accelerating factor 1(Daf1/CD55), by identifying DE specific genes from the expression profile of DE derived from mouse embryonic stem cells (ESCs) by microarray analysis, and in situ hybridization of early embryos. Daf1 is expressed in a subpopulation of E-cadherin + Cxcr4+ DE cells. The characteristics of the Daf1-expressing cells during DE differentiation has not been examined.
  
  
  
  In this report, we utilized the ESC differentiation system to examine the characteristics of Daf1-expressing DE cells. We found that Daf1 expression could discriminate late DE from early DE. Early DE cells are Daf1-negative (DE-) and late DE cells are Daf1-positive (DE+). We also found that Daf1+ late DE cells show low proliferative and low cell matrix adhesive characteristics. Furthermore, the purified SOX17(low) early DE cells gave rise to Daf1+ Sox17(high) late DE cells.
  
  
  
  Daf1-expressing late definitive endoderm proliferates slowly and show low adhesive capacity.
authors.affiliation: School of Life Science and Technology, Tokyo Institute of Technology, 4259-B-25 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa, 226-8501, Japan.|Stem Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University, Honjo 2-2-1, Kumamoto, 860-0811, Japan.|Division of Pharmacology, National Institute of Health Science, 1-18-1 Kamiyoga Setagaya-ku, Tokyo, 158-8501, Japan.
authors.forename: Soichiro
authors.initials: S
authors.lastname: Ogaki
authors.affiliation: Stem Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University, Honjo 2-2-1, Kumamoto, 860-0811, Japan.
authors.forename: Hisayoshi
authors.initials: H
authors.lastname: Omori
authors.affiliation: Stem Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University, Honjo 2-2-1, Kumamoto, 860-0811, Japan.
authors.forename: Mayu
authors.initials: M
authors.lastname: Morooka
authors.affiliation: School of Life Science and Technology, Tokyo Institute of Technology, 4259-B-25 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa, 226-8501, Japan.
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Shiraki
authors.affiliation: Division of Pharmacology, National Institute of Health Science, 1-18-1 Kamiyoga Setagaya-ku, Tokyo, 158-8501, Japan.
authors.forename: Seiichi
authors.initials: S
authors.lastname: Ishida
authors.affiliation: School of Life Science and Technology, Tokyo Institute of Technology, 4259-B-25 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa, 226-8501, Japan. skume@bio.titech.ac.jp.|Stem Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University, Honjo 2-2-1, Kumamoto, 860-0811, Japan. skume@bio.titech.ac.jp.
authors.forename: Shoen
authors.initials: S
authors.lastname: Kume
mesh_terms: D000818:Animals; D018958:CD55 Antigens / Q000378:metabolism*; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D004707:Endoderm / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D015398:Signal Transduction
chemical_list: D018958:CD55 Antigens; C521184:decay-accelerating factor 1, mouse
keywords: Adhesion; Daf1; Definitive endoderm; In vitro differentiation; Pluripotent stem cell; Proliferation