title: Microsatellite loci discovery from next-generation sequencing data and loci characterization in the epizoic barnacle Chelonibia testudinaria (Linnaeus, 1758).
abstract: Microsatellite markers remain an important tool for ecological and evolutionary research, but are unavailable for many non-model organisms. One such organism with rare ecological and evolutionary features is the epizoic barnacle Chelonibia testudinaria (Linnaeus, 1758). Chelonibia testudinaria appears to be a host generalist, and has an unusual sexual system, androdioecy. Genetic studies on host specificity and mating behavior are impeded by the lack of fine-scale, highly variable markers, such as microsatellite markers. In the present study, we discovered thousands of new microsatellite loci from next-generation sequencing data, and characterized 12 loci thoroughly. We conclude that 11 of these loci will be useful markers in future ecological and evolutionary studies on C. testudinaria.
authors.affiliation: Evolution and Ecology, University of California, Davis, CA, United States; Department of Genetics, University of Georgia, Athens, GA, United States.
authors.forename: Christine
authors.initials: C
authors.lastname: Ewers-Saucedo
authors.affiliation: Department of Biology, The Citadel , Charleston, SC , United States.
authors.forename: John D
authors.initials: JD
authors.lastname: Zardus
authors.affiliation: Department of Genetics, University of Georgia, Athens, GA, United States; Odum School of Ecology, University of Georgia, Athens, Georgia, United States.
authors.forename: John P
authors.initials: JP
authors.lastname: Wares
keywords: HWE; Homozygote excess; MiSeq; Microsatellite markers; Null alleles; PALFinder; R environment