title: [Xenopus laevis peroxiredoxins: Gene expression during development and characterization of the enzymes].
abstract: Reactive oxygen species (ROS) are produced via catabolic and anabolic processes during normal embryonic development, and ROS content in the cell is maintained at a certain level. Peroxiredoxins are a family of selenium-independent peroxidases and play a key role in maintaining redox homeostasis of the cell. In addition to regulating the ROS level, peroxiredoxins are involved in intracellular and intercellular signaling, cell differentiation, and tissue development. The time course of peroxiredoxin gene (prx1-6) expression was studied in Xenopus laevis during early ontogeny (Nieuwkoop and Faber stages 10-63). The highest expression level was observed for prx1 at these developmental stages. The prx1, prx3, and prx4 expression level changed most dramatically in response to oxidative stress artificially induced in X. laevis embryos. In X. laevis adults, prx1-6 were all intensely expressed in all organs examined, the prx1 expression level being the highest. The X. laevis prx1-6 genes were cloned and expressed in Escherichia coli, and physico-chemical characteristics were compared for the recombinant enzymes. The highest peroxidase activity and thermal stability were observed for Prx1 and Prx2. It was assumed that Prx1 plays a leading role in X. laevis early development.
authors.affiliation: Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow region, 142290, Russia.|sharapov.mg@yandex.ru.
authors.forename: M G
authors.initials: MG
authors.lastname: Sharapov
authors.affiliation: Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow region, 142290, Russia.
authors.forename: V I
authors.initials: VI
authors.lastname: Novoselov
authors.affiliation: Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow region, 142290, Russia.
authors.forename: V K
authors.initials: VK
authors.lastname: Ravin
mesh_terms: D000818:Animals; D003593:Cytoplasm / Q000235:genetics; D004625:Embryo, Nonmammalian; D047108:Embryonic Development / Q000235:genetics; D004795:Enzyme Stability; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000235:genetics*; D018384:Oxidative Stress / Q000235:genetics*; D010544:Peroxidases / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D054464:Peroxiredoxins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D017382:Reactive Oxygen Species / Q000378:metabolism; D029867:Xenopus Proteins / Q000235:genetics*; D014982:Xenopus laevis / Q000235:genetics / Q000254:growth & development*
chemical_list: D018398:Homeodomain Proteins; C000608632:Prx1 protein, Xenopus laevis; D017382:Reactive Oxygen Species; D029867:Xenopus Proteins; D010544:Peroxidases; D054464:Peroxiredoxins
keywords: Peroxiredoxins; Xenopus laevis; embryonic development; gene expression; reactive oxygen species