title: The control of hyperglycemia by a novel trypsin resistant oral insulin preparation in alloxan induced type I diabetic mice.
abstract: A trypsin resistant oral insulin preparation was made by incubating insulin for 2 h at 23 °C with previously boiled cow milk at 100 °C that was coagulated with 0.6 M acetic acid. The precipitate was resuspended in the same volume of milk. The immunoblot analysis of the suspended proteins treated with 200 ng of trypsin/ml for 3 h demonstrated that the 80.1% of the insulin in the suspension survived the proteolytic degradation compared to 0% of the hormone survived in the control. The feeding of 0.4 ml (0.08 unit of insulin) of the resuspended proteins followed by 0.2 ml of the same protein to alloxan induced diabetic mice maximally decreased the blood glucose level from 508 ± 10 mg/dl to 130 ± 10 mg/dl in 7 h with simultaneous increase of the basal plasma concentration of insulin from 3 ± 1.1 μunits/ml to 18 ± 1.5 μunits/ml. In control experiment the absence of insulin in the identical milk suspension produced no hypoglycemic effect suggesting milk was not responsible for the hypoglycemic effect of milk-insulin complex. Coming out of insulin-casein complex from the intestinal gut to the circulation was spontaneous and facilitated diffusion transportation which was found from Gibbs free energy reaction.
authors.affiliation: Sinha Institute of Medical Science &Technology, 288-Kendua main road, Baishnabghata, Garia, Kolkata-700084, India.|Cell &Molecular Therapeutic Lab, Dept. of Biochemistry, Vidyasagar University, Midnapur-721102, India.
authors.forename: Sarbashri
authors.initials: S
authors.lastname: Bank
authors.affiliation: Sinha Institute of Medical Science &Technology, 288-Kendua main road, Baishnabghata, Garia, Kolkata-700084, India.
authors.forename: Arjun
authors.initials: A
authors.lastname: Ghosh
authors.affiliation: Sinha Institute of Medical Science &Technology, 288-Kendua main road, Baishnabghata, Garia, Kolkata-700084, India.
authors.forename: Suman
authors.initials: S
authors.lastname: Bhattacharya
authors.affiliation: Cell &Molecular Therapeutic Lab, Dept. of Biochemistry, Vidyasagar University, Midnapur-721102, India.
authors.forename: Smarajit
authors.initials: S
authors.lastname: Maiti
authors.affiliation: Defence Institute of Physiology and Allied Sciences, Lucknow Road, Timarpur, Delhi-54, India.
authors.forename: Gausal A
authors.initials: GA
authors.lastname: Khan
authors.affiliation: Sinha Institute of Medical Science &Technology, 288-Kendua main road, Baishnabghata, Garia, Kolkata-700084, India.
authors.forename: Asru K
authors.initials: AK
authors.lastname: Sinha
mesh_terms: D000284:Administration, Oral; D000496:Alloxan; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D002364:Caseins / Q000378:metabolism; D002417:Cattle; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D004355:Drug Stability; D007328:Insulin / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics / Q000627:therapeutic use*; D007421:Intestine, Small / Q000378:metabolism; D051379:Mice; D008892:Milk; D011485:Protein Binding; D014018:Tissue Distribution; D014357:Trypsin / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D002364:Caseins; D007328:Insulin; D000496:Alloxan; D014357:Trypsin