title: Poring over two-pore channel pore mutants.
abstract: Two-pore channels are members of the voltage-gated ion channel superfamily. They localise to the endolysosomal system and are likely targets for the Ca2+mobilising messenger NAADP. In this brief review, we relate mutagenesis of the TPC pore to a recently published homology model and discuss how pore mutants are informing us of TPC function. Molecular physiology of these ubiquitous proteins is thus emerging.
authors.affiliation: Department of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Penny
authors.affiliation: Department of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT.
authors.forename: Sandip
authors.initials: S
authors.lastname: Patel
keywords: NAADP; TPC1; TPC2; homology modelling; site-directed mutagenesis; structure-function; voltage-gated ion channel