title: Transfection of Human Keratinocytes with Nucleoside-Modified mRNA Encoding CPD-Photolyase to Repair DNA Damage.
abstract: In vitro-synthesized mRNA containing nucleoside modifications has great therapeutical potential to transiently express proteins with physiological importance. One such protein is photolyase which rapidly removes UV-induced DNA damages, but this enzyme is absent in humans. Here, we apply a novel mRNA-based platform to achieve functional nonhuman photolyase production in cultured human keratinocytes. Transfection of nucleoside-modified mRNA encoding photolyase leads to accelerated repair of DNA photolesions in human keratinocytes.
authors.affiliation: Department of Dermatology, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98, Debrecen, 4032, Hungary.
authors.forename: Gábor
authors.initials: G
authors.lastname: Boros
authors.affiliation: Department of Neurosurgery, University of Pennsylvania, 3450 Hamilton Walk, Philadelphia, PA, 19104, USA.|BioNTech RNA Pharmaceuticals, An der Goldgrube 12, Mainz, 55131, Germany.
authors.forename: Katalin
authors.initials: K
authors.lastname: Karikó
authors.affiliation: Department of Neurosurgery, University of Pennsylvania, 3450 Hamilton Walk, Philadelphia, PA, 19104, USA.|BioNTech RNA Pharmaceuticals, An der Goldgrube 12, Mainz, 55131, Germany.
authors.forename: Hiromi
authors.initials: H
authors.lastname: Muramatsu
authors.affiliation: Department of Dermatology, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98, Debrecen, 4032, Hungary.|MTA-DE Lendület Laboratory of Cellular Metabolism Research Group of the Hungarian Academy of Sciences, University of Debrecen, Nagyerdei krt. 98, Debrecen, 4032, Hungary.
authors.forename: Edit
authors.initials: E
authors.lastname: Miko
authors.affiliation: Department of Dermatology, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98, Debrecen, 4032, Hungary.
authors.forename: Eszter
authors.initials: E
authors.lastname: Emri
authors.affiliation: Department of Dermatology, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98, Debrecen, 4032, Hungary.
authors.forename: Csaba
authors.initials: C
authors.lastname: Hegedűs
authors.affiliation: Department of Dermatology, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98, Debrecen, 4032, Hungary.
authors.forename: Gabriella
authors.initials: G
authors.lastname: Emri
authors.affiliation: Department of Dermatology, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98, Debrecen, 4032, Hungary. eremenyik@med.unideb.hu.
authors.forename: Éva
authors.initials: É
authors.lastname: Remenyik
mesh_terms: D002460:Cell Line; D004247:DNA / Q000528:radiation effects; D004249:DNA Damage; D004260:DNA Repair; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000378:metabolism; D009705:Nucleosides / Q000378:metabolism*; D012333:RNA, Messenger / Q000737:chemistry* / Q000378:metabolism; D014162:Transfection
chemical_list: D009705:Nucleosides; D012333:RNA, Messenger; D004247:DNA; D004255:Deoxyribodipyrimidine Photo-Lyase
keywords: Cyclobutane pyrimidine dimer-specific photolyase; DNA repair; Human keratinocytes; Nonviral gene therapy; Pseudouridine-modified mRNA; Transient transfection; Ultraviolet B