title: Genome stability versus transcript diversity.
abstract: Our genome is protected from the introduction of mutations by high fidelity replication and an extensive network of DNA damage response and repair mechanisms. However, the expression of our genome, via RNA and protein synthesis, allows for more diversity in translating genetic information. In addition, the splicing process has become less stringent over evolutionary time allowing for a substantial increase in the diversity of transcripts generated. The result is a diverse transcriptome and proteome that harbor selective advantages over a more tightly regulated system. Here, we describe mechanisms in place that both safeguard the genome and promote translational diversity, with emphasis on post-transcriptional RNA processing.
authors.affiliation: Department of Radiation Oncology, University of Michigan Comprehensive Cancer Center, and Translational Oncology Program, University of Michigan, Ann Arbor, USA; Department of Environmental Health Sciences, School of Public Health, University of Michigan, Ann Arbor, USA.
authors.forename: Brian
authors.initials: B
authors.lastname: Magnuson
authors.affiliation: Department of Radiation Oncology, University of Michigan Comprehensive Cancer Center, and Translational Oncology Program, University of Michigan, Ann Arbor, USA.
authors.forename: Karan
authors.initials: K
authors.lastname: Bedi
authors.affiliation: Department of Radiation Oncology, University of Michigan Comprehensive Cancer Center, and Translational Oncology Program, University of Michigan, Ann Arbor, USA; Department of Environmental Health Sciences, School of Public Health, University of Michigan, Ann Arbor, USA. Electronic address: ljungman@umich.edu.
authors.forename: Mats
authors.initials: M
authors.lastname: Ljungman
mesh_terms: D017398:Alternative Splicing; D004249:DNA Damage; D004260:DNA Repair; D004261:DNA Replication; D020869:Gene Expression Profiling; D014644:Genetic Variation; D015894:Genome, Human; D042822:Genomic Instability; D006801:Humans; D014176:Protein Biosynthesis; D020543:Proteome; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D059467:Transcriptome
chemical_list: D020543:Proteome; D012333:RNA, Messenger
keywords: DNA damage response; DNA repair; Polymerase fidelity; Post-transcriptional RNA processing; Protein translation; Proteomic diversity; RNA splicing; RNA transcription