title: Vitamin C promotes the proliferation of human adipose-derived stem cells via p53-p21 pathway.
abstract: Although adipose-derived stem cells (ADSCs) have demonstrated a promising potential for the applications of cell-based therapy and regenerative medicine, excessive reactive oxygen species (ROS) are harmful to ADSCs cell survival and proliferation. Vitamin C is an important antioxidant, and is often added into culture media as an essential micronutrient. However, its roles on the proliferation of human ADSCs have not been studied. Therefore, in this study, human ADSCs were isolated, and detected by flow cytometry for the analysis of their cell surface antigens. Cell proliferation and cell cycle progression were measured with cell counting kit-8 assay and flow cytometry, respectively. Western blotting was used to detect the expression levels of cyclin E1, p53, p21, and CDK2 proteins. The effect of vitamin C pretreatment on the production of hydrogen peroxide (H2O2)-mediated ROS in the ADSCs was evaluated by flow cytometry. Our results indicated that vitamin C treatment significantly increased cell proliferation, and changed the cell cycle distribution of ADSCs by decreasing the percentage of G1phase, and concurrently increased the percentage of S and G2/M phase. Western blot analysis indicated that vitamin C treatment up-regulated the expression levels of cyclin E1 and CDK2, but down-regulated p53 and p21 proteins expression, which contributed to cell proliferation and cell cycle progression. Vitamin C pretreatment significantly reduced the production of H2O2-induced ROS in the ADSCs. These findings suggest that vitamin C can promote the proliferation and cell cycle progression in the ADSCs possibly through regulation of p53-p21 signal pathway.
authors.affiliation: a Institute of Plastic Surgery, Affiliated Hospital of Guangdong Medical University , Zhanjiang, Guangdong Province , China.
authors.forename: Peihua
authors.initials: P
authors.lastname: Zhang
authors.affiliation: a Institute of Plastic Surgery, Affiliated Hospital of Guangdong Medical University , Zhanjiang, Guangdong Province , China.
authors.forename: Jin
authors.initials: J
authors.lastname: Li
authors.affiliation: a Institute of Plastic Surgery, Affiliated Hospital of Guangdong Medical University , Zhanjiang, Guangdong Province , China.
authors.forename: Yawei
authors.initials: Y
authors.lastname: Qi
authors.affiliation: a Institute of Plastic Surgery, Affiliated Hospital of Guangdong Medical University , Zhanjiang, Guangdong Province , China.
authors.forename: Yaqing
authors.initials: Y
authors.lastname: Zou
authors.affiliation: a Institute of Plastic Surgery, Affiliated Hospital of Guangdong Medical University , Zhanjiang, Guangdong Province , China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: b Institute of Biochemistry and Molecular Biology, Guangdong Medical University , Zhanjiang, Guangdong Province , China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Tang
authors.affiliation: a Institute of Plastic Surgery, Affiliated Hospital of Guangdong Medical University , Zhanjiang, Guangdong Province , China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Duan
authors.affiliation: c Department of Plastic Surgery , the First Affiliated Hospital of Jinan University, Key Laboratory for Regenerative Medicine, Ministry of Education , Guangzhou, Guangdong Province , China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Liu
authors.affiliation: a Institute of Plastic Surgery, Affiliated Hospital of Guangdong Medical University , Zhanjiang, Guangdong Province , China.
authors.forename: Guofang
authors.initials: G
authors.lastname: Zeng
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000954:Antigens, Surface / Q000378:metabolism; D001205:Ascorbic Acid / Q000494:pharmacology*; D015415:Biomarkers / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002469:Cell Separation; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D000954:Antigens, Surface; D015415:Biomarkers; D050759:Cyclin-Dependent Kinase Inhibitor p21; D017382:Reactive Oxygen Species; D016159:Tumor Suppressor Protein p53; D006861:Hydrogen Peroxide; D001205:Ascorbic Acid
keywords: adipose-derived stem cells; cell cycle; cell proliferation; reactive oxygen species; vitamin C