title: High hydrostatic pressure adaptive strategies in an obligate piezophile Pyrococcus yayanosii.
abstract: Pyrococcus yayanosii CH1, as the first and only obligate piezophilic hyperthermophilic microorganism discovered to date, extends the physical and chemical limits of life on Earth. It was isolated from the Ashadze hydrothermal vent at 4,100 m depth. Multi-omics analyses were performed to study the mechanisms used by the cell to cope with high hydrostatic pressure variations. In silico analyses showed that the P. yayanosii genome is highly adapted to its harsh environment, with a loss of aromatic amino acid biosynthesis pathways and the high constitutive expression of the energy metabolism compared with other non-obligate piezophilic Pyrococcus species. Differential proteomics and transcriptomics analyses identified key hydrostatic pressure-responsive genes involved in translation, chemotaxis, energy metabolism (hydrogenases and formate metabolism) and Clustered Regularly Interspaced Short Palindromic Repeats sequences associated with Cellular apoptosis susceptibility proteins.
authors.affiliation: Univ Brest, CNRS, Ifremer, UMR 6197-Laboratoire de Microbiologie des Environnements Extrêmes (LM2E), Institut Universitaire Européen de la Mer (IUEM), rue Dumont d'Urville, 29 280 Plouzané, France.
authors.forename: Grégoire
authors.initials: G
authors.lastname: Michoud
authors.affiliation: Univ Brest, CNRS, Ifremer, UMR 6197-Laboratoire de Microbiologie des Environnements Extrêmes (LM2E), Institut Universitaire Européen de la Mer (IUEM), rue Dumont d'Urville, 29 280 Plouzané, France.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Jebbar
mesh_terms: D000222:Adaptation, Physiological; D000596:Amino Acids / Q000096:biosynthesis; D019843:Archaeal Proteins / Q000235:genetics*; D053898:Biosynthetic Pathways; D019295:Computational Biology / Q000379:methods*; D003198:Computer Simulation; D019641:DNA, Archaeal / Q000235:genetics; D004734:Energy Metabolism; D020869:Gene Expression Profiling / Q000379:methods; D019848:Gene Expression Regulation, Archaeal; D006874:Hydrostatic Pressure; D060086:Hydrothermal Vents / Q000382:microbiology; D005810:Multigene Family; D040901:Proteomics / Q000379:methods; D019711:Pyrococcus / Q000235:genetics* / Q000302:isolation & purification; D013312:Stress, Physiological
chemical_list: D000596:Amino Acids; D019843:Archaeal Proteins; D019641:DNA, Archaeal