title: Chitosan-functionalised single-walled carbon nanotube-mediated drug delivery of SNX-2112 in cancer cells.
abstract: Delivery of amphiphobic drugs (insoluble in both water and oil) has been a great challenge in drug delivery. SNX-2112, a novel inhibitor of Hsp90, is a promising drug candidate for treating various types of cancers; however, the insolubility greatly limits its clinical application. This study aimed to build a new type of drug delivery system using single-walled carbon nanotubes (SWNTs) for controllable release of SNX-2112; chitosan (CHI) was non-covalently added to SWNTs to improve their biocompatibility. SWNTs-CHI demonstrated high drug-loading capability; the release of SNX-2112 was pH triggered and time related. The intracellular reactive oxygen species of SWNTs-CHI increased, compared with that of SWNTs, leading to higher mitogen-activated protein kinase and cell apoptosis. The results of western-blotting, lactate dehydrogenase (LDH) release assay, and cell viability assay analyses indicated that apoptosis-related proteins were abundantly expressed in K562 cells and that the drug delivery system significantly inhibited K562 cells. Thus, SWNT-CHI/SNX-2112 shows great potential as a drug delivery system for cancer therapy.
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Lixia
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Department of Pharmacy, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Shao
authors.initials: S
authors.lastname: Wu
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Li
authors.initials: L
authors.lastname: Tan
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China tanhuo.2008@163.com 10165608@qq.com.
authors.forename: Huo
authors.initials: H
authors.lastname: Tan
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Baodan
authors.initials: B
authors.lastname: Yu
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry; D017209:Apoptosis / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D003692:Delayed-Action Preparations / Q000008:administration & dosage* / Q000138:chemical synthesis; D004058:Diffusion; D006576:Heterocyclic Compounds, 4 or More Rings / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D020014:K562 Cells; D053769:Nanocapsules / Q000008:administration & dosage / Q000737:chemistry* / Q000648:ultrastructure; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000473:pathology; D010316:Particle Size; D016896:Treatment Outcome
chemical_list: D000970:Antineoplastic Agents; D003692:Delayed-Action Preparations; D006576:Heterocyclic Compounds, 4 or More Rings; D053769:Nanocapsules; D037742:Nanotubes, Carbon; C534922:SNX 2112; D048271:Chitosan
keywords: SNX-2112; Single-walled carbon nanotubes; cancer; chitosan; drug delivery; lactate dehydrogenase; reactive oxygen species