title: Degradation of synthetic pollutants in real wastewater using laccase encapsulated in core-shell magnetic copper alginate beads.
abstract: Immobilization of laccase has been highlighted to enhance their stability and reusability in bioremediation. In this study, we provide a novel immobilization technique that is very suitable to real wastewater treatment. A perfect core-shell system composing copper alginate for the immobilization of laccase (Lac-beads) was produced. Additionally, nFe2O3 was incorporated for the bead recycling through magnetic force. The beads were proven to immobilize 85.5% of total laccase treated and also to be structurally stable in water, acetate buffer, and real wastewater. To test the Lac-beads reactivity, triclosan (TCS) and Remazol Brilliant Blue R (RBBR) were employed. The Lac-beads showed a high percentage of TCS removal (89.6%) after 8h and RBBR decolonization at a range from 54.2% to 75.8% after 4h. Remarkably, the pollutants removal efficacy of the Lac-beads was significantly maintained in real wastewater with the bead recyclability, whereas that of the corresponding free laccase was severely deteriorated.
authors.affiliation: School of Environmental Science and Engineering, POSTECH, Pohang 790-784, Republic of Korea.
authors.forename: Thao Thanh
authors.initials: TT
authors.lastname: Le
authors.affiliation: Department of Environmental Science, Periyar University, Periyar Palkalai Nagar, Salem 636011, Tamil Nadu, India.
authors.forename: Kumarasamy
authors.initials: K
authors.lastname: Murugesan
authors.affiliation: School of Environmental Science and Engineering, POSTECH, Pohang 790-784, Republic of Korea.
authors.forename: Chung-Seop
authors.initials: CS
authors.lastname: Lee
authors.affiliation: School of Environmental Science and Engineering, POSTECH, Pohang 790-784, Republic of Korea.
authors.forename: Chi Huong
authors.initials: CH
authors.lastname: Vu
authors.affiliation: School of Environmental Science and Engineering, POSTECH, Pohang 790-784, Republic of Korea. Electronic address: yschang@postech.ac.kr.
authors.forename: Yoon-Seok
authors.initials: YS
authors.lastname: Chang
authors.affiliation: Department of Agricultural Chemistry & Life Sciences, IALS, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Jong-Rok
authors.initials: JR
authors.lastname: Jeon
mesh_terms: D000464:Alginates / Q000737:chemistry; D000880:Anthraquinones / Q000737:chemistry / Q000378:metabolism; D001673:Biodegradation, Environmental; D002617:Chemical Industry; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D042845:Laccase / Q000737:chemistry* / Q000378:metabolism; D008280:Magnetics; D014865:Waste Disposal, Fluid / Q000295:instrumentation* / Q000379:methods; D062065:Wastewater / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000464:Alginates; D000880:Anthraquinones; D004800:Enzymes, Immobilized; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D042845:Laccase; D062065:Wastewater; D014874:Water Pollutants, Chemical; C024758:Remazol Brilliant Blue R
keywords: Core–shell magnetic copper alginate beads; Immobilization; Laccase; Real wastewater; Water pollutants