title: Antimicrobial activity of tea catechin against canine oral bacteria and the functional mechanisms.
abstract: Epigallocatechin gallate (EGCG) is the major polyphenolic compound of green tea. Polyphenolic compounds were extracted from the leaf of Camellia sinensis (Japanese green tea), and the minimum inhibitory concentration against canine oral bacteria was measured. Subsequently, we investigated the inhibitory effects of polyphenolic compounds and EGCG on the growth of canine oral bacteria. EGCG showed antimicrobial activity against a model bacterium, Streptococcus mutans. Our results indicate that EGCG can inhibit the growth and biofilm formation of S. mutans and that EGCG does not interact with streptococcal lipoteichoic acid (LTA). Furthermore, our findings suggest that EGCG interacts with other component(s) of the bacterial membrane aside from streptococcal LTA to inhibit biofilm formation and damage biofilms.
authors.affiliation: Laboratory of Animal Microbiology, Department of Microbial Biotechnology, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori, Amamiya-machi Aoba-ku, Sendai, Miyagi 981-8555, Japan.
authors.forename: Lanlan
authors.initials: L
authors.lastname: Bai
authors.forename: Shiaki
authors.initials: S
authors.lastname: Takagi
authors.forename: Tasuke
authors.initials: T
authors.lastname: Ando
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yoneyama
authors.forename: Kumiko
authors.initials: K
authors.lastname: Ito
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Mizugai
authors.forename: Emiko
authors.initials: E
authors.lastname: Isogai
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects; D028241:Camellia sinensis / Q000737:chemistry*; D002392:Catechin / Q000031:analogs & derivatives / Q000494:pharmacology*; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D008826:Microbial Sensitivity Tests; D064307:Microbiota / Q000187:drug effects*; D009055:Mouth / Q000382:microbiology*; D018515:Plant Leaves / Q000737:chemistry*; D013295:Streptococcus mutans / Q000187:drug effects; D013662:Tea / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D013662:Tea; D002392:Catechin; C045651:epigallocatechin gallate