title: Silencing of ABCG2 by MicroRNA-3163 Inhibits Multidrug Resistance in Retinoblastoma Cancer Stem Cells.
abstract: To investigate the function and regulation mechanism of ATP-binding cassette, subfamily G, member 2 (ABCG2) in retinoblastoma cancer stem cells (RCSCs), a long-term culture of RCSCs from WERI-Rb1 cell line was successfully established based on the high expression level of ABCG2 on the surface of RCSCs. To further explore the molecular mechanism of ABCG2 on RCSCs, a microRNA that specifically targets ABCG2 was predicted. Subsequently, miR-3163 was selected and confirmed as the ABCG2-regulating microRNA. Overexpression of miR-3163 led to a significant decrease in ABCG2 expression. Additionally, ABCG2 loss-of-function induced anti-proliferation and apoptosis-promoting functions in RCSCs, and multidrug resistance to cisplatin, carboplatin, vincristine, doxorubicin, and etoposide was greatly improved in these cells. Our data suggest that miR-3163 has a significant impact on ABCG2 expression and can influence proliferation, apoptosis, and drug resistance in RCSCs. This work may provide new therapeutic targets for retinoblastoma.
authors.affiliation: Department of Ophthalmology, Linzi District People's Hospital, Zibo City, Shandong Province, P. R. China .
authors.forename: Ming
authors.initials: M
authors.lastname: Jia
authors.affiliation: Department of Ophthalmology, Linzi District People's Hospital, Zibo City, Shandong Province, P. R. China .
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Wei
authors.affiliation: Department of Ophthalmology, Linzi District People's Hospital, Zibo City, Shandong Province, P. R. China .
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.affiliation: Department of Ophthalmology, Linzi District People's Hospital, Zibo City, Shandong Province, P. R. China .
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zhao
mesh_terms: D020413:3' Untranslated Regions; D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D000070416:Antagomirs / Q000378:metabolism; D000970:Antineoplastic Agents / Q000633:toxicity; D017209:Apoptosis / Q000187:drug effects; D001483:Base Sequence; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D019008:Drug Resistance, Neoplasm / Q000187:drug effects; D020868:Gene Silencing; D006801:Humans; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D012175:Retinoblastoma / Q000378:metabolism / Q000473:pathology; D016415:Sequence Alignment; D014162:Transfection
chemical_list: D020413:3' Untranslated Regions; C116614:ABCG2 protein, human; D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D000070416:Antagomirs; D000970:Antineoplastic Agents; D035683:MicroRNAs; D009363:Neoplasm Proteins
keywords: ABCG2; RCSCs; Retinoblastoma; miR-3163