title: Establishment and application of a real-time loop-mediated isothermal amplification system for the detection of CYP2C19 polymorphisms.
abstract: Single-nucleotide polymorphisms (SNPs) represent the most widespread type of genetic variation (approximately 90%) in the human genome, and the demand to overcome such variation has received more attention now than ever before. The capacity to rapidly assess SNPs that correlate with disease predisposition, drug efficacy and drug toxicity is a key step for the development of personalized medicine. In this work, a rapid one-step SNP detection method, real-time loop-mediated isothermal amplification (RT-LAMP), was first applied for CYP2C19 polymorphisms testing. The optimized method was established with specifically designed primers for target amplification by real-time detection in approximately 30 min under isothermal conditions. RT-LAMP amplified few copies of template to produce significant amounts of product and quantitatively detected human DNA with compatible specificity and sensitivity. The success in the establishment of this RT-LAMP protocol for CYP2C19 polymorphism testing is significant for the extension of this technique for the detection of other SNPs, which will further facilitate the development of personalized medicine.
authors.affiliation: College of Life Sciences, Northwest University, Xi'an, 710069, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.affiliation: National Engineering Research Center for Miniaturized Detection Systems, Xi'an, 710069, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Yao
authors.affiliation: National Engineering Research Center for Miniaturized Detection Systems, Xi'an, 710069, China.
authors.forename: Juan-Li
authors.initials: JL
authors.lastname: Zhu
authors.affiliation: College of Life Sciences, Northwest University, Xi'an, 710069, China.
authors.forename: Si-Nong
authors.initials: SN
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Northwest University, Xi'an, 710069, China.
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Northwest University, Xi'an, 710069, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wei
authors.affiliation: College of Life Sciences, Northwest University, Xi'an, 710069, China.|National Engineering Research Center for Miniaturized Detection Systems, Xi'an, 710069, China.
authors.forename: Wen-Li
authors.initials: WL
authors.lastname: Hui
authors.affiliation: College of Life Sciences, Northwest University, Xi'an, 710069, China.|National Engineering Research Center for Miniaturized Detection Systems, Xi'an, 710069, China.
authors.forename: Ya-Li
authors.initials: YL
authors.lastname: Cui
mesh_terms: D065731:Cytochrome P-450 CYP2C19 / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D020641:Polymorphism, Single Nucleotide; D057285:Precision Medicine; D013696:Temperature
chemical_list: D017931:DNA Primers; C045793:CYP2C19 protein, human; D065731:Cytochrome P-450 CYP2C19