title: Micro-Nano-Bio Diagnostic System for Food Pathogen Detection Revolutionizes Food Safety Management & Protects Consumers Health.
abstract: The development of integrated, fast and affordable platforms for pathogen detection is an emerging area where a multidisciplinary approach is necessary for designing microsystems employing miniaturized devices; these new technologies promise a significant advancement of the current state of analytical testing leading to improved healthcare. In this work, the development of a lab-on-chip microsystem platform for the genetic analysis of Salmonella in milk samples is presented. The heart of the platform is an acoustic detection biochip, integrated with a microfluidic module. This detection platform is combined with a micro-processor, which, alongside with magnetic beads technology and a DNA micro-amplification module, are responsible for performing sample pre-treatment, bacteria lysis, nucleic acid purification and amplification. Automated, multiscale manipulation of fluids in complex microchannel networks is combined with novel sensing principles developed by some of the partners. This system is expected to have a significant impact in food-pathogen detection by providing for the first time an integrated detection test for Salmonella screening in a very short time. Finally, thanks to the low cost and compact technologies involved, the proposed set-up is expected to provide a competitive analytical platform for direct application in field settings.
authors.affiliation: Institute of Nanoscience and Nanotechnology, NCSR-Demokritos, Patriarhou Gregoriou Str., Aghia Paraskevi 15310, Greece.
authors.forename: Evangelos
authors.initials: E
authors.lastname: Gogolides
authors.affiliation: Institute of Nanoscience and Nanotechnology, NCSR-Demokritos, Patriarhou Gregoriou Str., Aghia Paraskevi 15310, Greece.
authors.forename: Angeliki
authors.initials: A
authors.lastname: Tserepi
authors.affiliation: Jobst Technologies GMBH, 4B Engesserstrasse, Freiburg 79108, Germany.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Jobst
authors.affiliation: SENSeOR SAS, 18 Temis Innovation, rue Alain Savary, 25000 Besançon, France.
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Friedt
authors.affiliation: SENSeOR SAS, 18 Temis Innovation, rue Alain Savary, 25000 Besançon, France.
authors.forename: David
authors.initials: D
authors.lastname: Rabus
authors.affiliation: Inst. Pasteur, 25-28 Rue du Docteur Roux, Paris Cedex 15, 75724 France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Dupuy
authors.affiliation: University of Pardubice, 573 Studentska, Pardubice 532 10, Czech Republic.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Bilkova
authors.affiliation: Institut Curie, 26 Rue d'Ulm, Paris 75231, France.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Descroix
authors.affiliation: Institut Curie, 26 Rue d'Ulm, Paris 75231, France.
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Viovy
authors.affiliation: Institute of Molecular Biology and Biotechnology-FORTH, 100 N. Plastira Str., Heraklion, 70013, Greece.
authors.forename: George
authors.initials: G
authors.lastname: Papadakis
authors.affiliation: Institute of Molecular Biology and Biotechnology-FORTH, 100 N. Plastira Str., Heraklion, 70013, Greece.
authors.forename: Electra
authors.initials: E
authors.lastname: Gizeli
mesh_terms: D000818:Animals; D004269:DNA, Bacterial / Q000032:analysis; D005516:Food Microbiology / Q000379:methods*; D056656:Lab-On-A-Chip Devices / Q000382:microbiology*; D008892:Milk / Q000382:microbiology*; D012475:Salmonella / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial