title: Mitotic phosphorylation of SOX2 mediated by Aurora kinase A is critical for the stem-cell like cell maintenance in PA-1 cells.
abstract: Transcription factor SOX2 is multiple phosphorylated. However, the kinase and the timing regulating SOX2 phosphorylation remains poorly understood. Here we reported mitotic phosphorylation of SOX2 by Aurora kinase A (AURKA). AURKA inhibitors (VX680, Aurora kinase Inhibitor I) but not PLK1 inhibitors (BI2536, CBB2001) eliminate the mitotic phosphorylation of SOX2. Consistently, siRNA inhibition of AURKA can eliminate mitotic SOX2 phosphorylation. Ser220 and Ser251 are two sites that identified for mitotic phosphorylation on SOX2. Moreover, SOX2 mutants (S220A and S251A) can promote SOX2 induced OCT4 re-expression in differentiated cells. These findings reveal a novel regulation mechanism of SOX2 phosphorylation mediated by AURKA in mitosis and its function in stem cell pluripotency maintenance in cancer cells.
authors.affiliation: a Laboratory of Chemical Genomics, School of Chemical Biology & Biotechnology, Peking University Shenzhen Graduate School , Shenzhen , China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Qi
authors.affiliation: a Laboratory of Chemical Genomics, School of Chemical Biology & Biotechnology, Peking University Shenzhen Graduate School , Shenzhen , China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: a Laboratory of Chemical Genomics, School of Chemical Biology & Biotechnology, Peking University Shenzhen Graduate School , Shenzhen , China.
authors.forename: Min
authors.initials: M
authors.lastname: Yu
authors.affiliation: b Institute of Research and Continuing Education, Hong Kong Baptist University , Shenzhen , China.
authors.forename: Rongfeng
authors.initials: R
authors.lastname: Lan
authors.affiliation: c Key Laboratory of Marine Bioresources and Ecology, College of Life Science, Shenzhen University , Shenzhen , China.
authors.forename: Shuiming
authors.initials: S
authors.lastname: Li
authors.affiliation: a Laboratory of Chemical Genomics, School of Chemical Biology & Biotechnology, Peking University Shenzhen Graduate School , Shenzhen , China.
authors.forename: Fei
authors.initials: F
authors.lastname: Lu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D064096:Aurora Kinase A / Q000378:metabolism*; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D065150:Cellular Reprogramming; D006801:Humans; D051379:Mice; D008938:Mitosis; D008954:Models, Biological; D050505:Mutant Proteins / Q000378:metabolism; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D010766:Phosphorylation; D010768:Phosphoserine / Q000378:metabolism; D055748:SOXB1 Transcription Factors / Q000737:chemistry / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*
chemical_list: D050505:Mutant Proteins; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D055748:SOXB1 Transcription Factors; D010768:Phosphoserine; D064096:Aurora Kinase A
keywords: AURKA; OCT4; PA-1 cell; SOX2; phosphorylation