title: [Resistance risk, cross-resistance and biochemical resistance mechanism of Laodelphax striatellus to buprofezin].
abstract: In order to investigate the resistance development law and biochemical resistance mechanism of Laodelphax striatellus to buprofezin, spraying rice seedlings was used to continuously screen resistant strains of L. striatellus and dipping rice seedlings was applied to determine the toxicity and cross-resistance of L. striatellus to insecticides. After 32-generation screening with buprofezin, L. striatellus developed 168.49 folds resistance and its reality heritability (h2) was 0.11. If the killing rate was 80%-90%, L. striatellus was expected to develop 10-fold resistance to buprofezin only after 5 to 6 generations breeding. Because the actual reality heritability of field populations was usually lower than that of the resistant strains, the production of field populations increasing with 10-fold resistance would need much longer time. The results of cross-resistance showed that resistant strain had high level cross-resistance with thiamethoxam and imidacloprid, low level cross-resistance with acetamiprid, and no cross-resistance with pymetrozine and chlorpyrifos. The activity of detoxification enzymes of different strains and the syergism of synergist were measured. The results showed that cytochrome P450 monooxygenase played a major role in the resistance of L. striatellus to buprofezin, the esterase played a minor role and the GSH-S-transferase had no effect. Therefore, L. striatellus would have high risk to develop resistance to buprofezin when used in the field and might be delayed by using pymetrozine and chlorpyrifos.
authors.forename: Xu-lian
authors.initials: XL
authors.lastname: Mao
authors.forename: Jin
authors.initials: J
authors.lastname: Liu
authors.forename: Xu-ke
authors.initials: XK
authors.lastname: Li
authors.forename: Jia-jia
authors.initials: JJ
authors.lastname: Chi
authors.forename: Yong-jie
authors.initials: YJ
authors.lastname: Liu
mesh_terms: D000818:Animals; D004390:Chlorpyrifos; D006430:Hemiptera; D007093:Imidazoles; D007305:Insecticide Resistance; D007306:Insecticides / Q000737:chemistry*; D000073943:Neonicotinoids; D009574:Nitro Compounds; D012275:Oryza; D010078:Oxazines; D013829:Thiadiazines / Q000737:chemistry*; D000077922:Thiamethoxam; D013844:Thiazoles; D014227:Triazines
chemical_list: D007093:Imidazoles; D007306:Insecticides; D000073943:Neonicotinoids; D009574:Nitro Compounds; D010078:Oxazines; D013829:Thiadiazines; D013844:Thiazoles; D014227:Triazines; C083997:buprofezin; C082359:imidacloprid; D000077922:Thiamethoxam; C480311:pymetrozine; D004390:Chlorpyrifos