title: Experimental strategies towards increasing intracellular mitochondrial activity in oocytes: A systematic review.
abstract: The mitochondrial complement is critical in sustaining the earliest stages of life. To improve the Assisted Reproductive Technology (ART), current methods of interest were evaluated for increasing the activity and copy number of mitochondria in the oocyte cell.
  
  
  
  This covered the researches from 1966 to September 2015.
  
  
  
  The results provided ten methods that can be studied individually or simultaneously.
  
  
  
  Though the use of these techniques generated great concern about heteroplasmy observation in humans, it seems that with study on these suggested methods there is real hope for effective treatments of old oocyte or oocytes containing mitochondrial problems in the near future.
authors.affiliation: Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran. Electronic address: saradarbandi@hotmail.com.
authors.forename: Sara
authors.initials: S
authors.lastname: Darbandi
authors.affiliation: Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran. Electronic address: mahsadarbandi@hotmail.com.
authors.forename: Mahsa
authors.initials: M
authors.lastname: Darbandi
authors.affiliation: Genetics Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran. Electronic address: hrkk1@uswr.ac.ir.
authors.forename: Hamid Reza Khorram
authors.initials: HR
authors.lastname: Khorshid
authors.affiliation: Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran. Electronic address: Sadeghi@Avicenna.ac.ir.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Sadeghi
authors.affiliation: Reproductive Medicine Unit, University of Schleswig-Holstein, Luebeck, Germany. Electronic address: sf_alhasani@hotmail.com.
authors.forename: Safaa
authors.initials: S
authors.lastname: Al-Hasani
authors.affiliation: Center for Reproductive Medicine, Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, OH, USA. Electronic address: AGARWAA@ccf.org.
authors.forename: Ashok
authors.initials: A
authors.lastname: Agarwal
authors.affiliation: Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran. Electronic address: shiraziabbas@yahoo.com.
authors.forename: Abolfazl
authors.initials: A
authors.lastname: Shirazi
authors.affiliation: Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran. Electronic address: M.@avicenna.ar.ir.
authors.forename: Mahnaz
authors.initials: M
authors.lastname: Heidari
authors.affiliation: Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran. Electronic address: Akhondi@Avicenna.ac.ir.
authors.forename: Mohammad Mehdi
authors.initials: MM
authors.lastname: Akhondi
mesh_terms: D035843:Biomedical Research / Q000639:trends; D006801:Humans; D008928:Mitochondria / Q000378:metabolism*; D009865:Oocytes / Q000502:physiology*; D001678:Organelle Biogenesis; D027724:Reproductive Techniques, Assisted
keywords: Micromanipulation; Mitochondrial activity; Mitochondrial copy number; Oocyte