title: Determination of Urine Albumin by New Simple High-Performance Liquid Chromatography Method.
abstract: BACKGROUND
  A simple high-performance liquid chromatography (HPLC) method was developed for the determination of albumin in patients' urine samples without coeluting proteins and was compared with the immunoturbidimetric determination of albumin. Urine albumin is important biomarker in diabetic patients, but part of it is immuno-nonreactive.
  
  
  METHODS
  Albumin was determined by high-performance liquid chromatography (HPLC), UV detection at 280 nm, Zorbax 300SB-C3 column. Immunoturbidimetric analysis was performed using commercial kit on automatic biochemistry analyzer COBAS INTEGRA®400, Roche Diagnostics GmbH, Manheim, Germany.
  
  
  RESULTS
  The HLPC method was fully validated. No significant interference with other proteins (transferrin, α-1-acid glycoprotein, α-1-antichymotrypsin, antitrypsin, hemopexin) was found. The results from 301 urine samples were compared with immunochemical determination. We found a statistically significant difference between these methods (P = 0.0001, Mann-Whitney test).
  
  
  CONCLUSIONS
  New simple HPLC method was developed for the determination of urine albumin without coeluting proteins. Our data indicate that the HPLC method is highly specific and more sensitive than immunoturbidimetry.
authors.affiliation: Department of Medical Chemistry and Clinical Biochemistry, 2nd Faculty of Medicine and University Hospital Motol, Charles University, Prague, Czech Republic. eva.klapkova@email.cz.
authors.forename: Eva
authors.initials: E
authors.lastname: Klapkova
authors.affiliation: Department of Medical Chemistry and Clinical Biochemistry, 2nd Faculty of Medicine and University Hospital Motol, Charles University, Prague, Czech Republic.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Fortova
authors.affiliation: Department of Medical Chemistry and Clinical Biochemistry, 2nd Faculty of Medicine and University Hospital Motol, Charles University, Prague, Czech Republic.
authors.forename: Richard
authors.initials: R
authors.lastname: Prusa
authors.affiliation: Department of Medical Chemistry and Clinical Biochemistry, 2nd Faculty of Medicine and University Hospital Motol, Charles University, Prague, Czech Republic.
authors.forename: Libuse
authors.initials: L
authors.lastname: Moravcova
authors.affiliation: Department of Medical Chemistry and Clinical Biochemistry, 2nd Faculty of Medicine and University Hospital Motol, Charles University, Prague, Czech Republic.
authors.forename: Karel
authors.initials: K
authors.lastname: Kotaska
mesh_terms: D000418:Albumins / Q000032:analysis*; D000419:Albuminuria / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D008297:Male; D013997:Time Factors; D016482:Urinalysis
chemical_list: D000418:Albumins
keywords: high-performance liquid chromatography; microalbuminuria; proteinuria; urine albumin