title: Applications and challenges in using LC-MS/MS assays for quantitative doping analysis.
abstract: LC-MS/MS is useful for qualitative and quantitative analysis of 'doped' biological samples from athletes. LC-MS/MS-based assays at low-mass resolution allow fast and sensitive screening and quantification of targeted analytes that are based on preselected diagnostic precursor-product ion pairs. Whereas LC coupled with high-resolution/high-accuracy MS can be used for identification and quantification, both have advantages and challenges for routine analysis. Here, we review the literature regarding various quantification methods for measuring prohibited substances in athletes as they pertain to World Anti-Doping Agency regulations.
authors.affiliation: Food & Drug Anti-doping Laboratory, China Anti-Doping Agency, 1st Anding Road, ChaoYang District, Beijing 100029, PR China.
authors.forename: Zhanliang
authors.initials: Z
authors.lastname: Wang
authors.affiliation: National Anti-doping Laboratory, China Anti-Doping Agency, 1st Anding Road, ChaoYang District, Beijing 100029, PR China.
authors.forename: Jianghai
authors.initials: J
authors.lastname: Lu
authors.affiliation: Food & Drug Anti-doping Laboratory, China Anti-Doping Agency, 1st Anding Road, ChaoYang District, Beijing 100029, PR China.
authors.forename: Yinong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: National Anti-doping Laboratory, China Anti-Doping Agency, 1st Anding Road, ChaoYang District, Beijing 100029, PR China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Tian
authors.affiliation: National Anti-doping Laboratory, China Anti-Doping Agency, 1st Anding Road, ChaoYang District, Beijing 100029, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yuan
authors.affiliation: National Anti-doping Laboratory, China Anti-Doping Agency, 1st Anding Road, ChaoYang District, Beijing 100029, PR China.
authors.forename: Youxuan
authors.initials: Y
authors.lastname: Xu
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D004300:Doping in Sports; D006801:Humans; D058573:Performance-Enhancing Substances / Q000097:blood* / Q000652:urine*; D015813:Substance Abuse Detection / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D058573:Performance-Enhancing Substances
keywords: LC–MS/MS; doping analysis; quantification