title: NMR-Based Metabolomic Analysis of Normal and Inflamed Gut.
abstract: Crohn's disease and ulcerative colitis, the two major forms of idiopathic inflammatory bowel disease (IBD), are thought to occur through a loss of intestinal barrier leading to an inappropriate immune response toward intestinal microbiota. While genome-wide association studies (GWAS) have provided much information about susceptibility loci associated with these diseases, the etiology of IBD is still unknown. Metabolomic analysis allows for the comprehensive measurement of multiple small molecule metabolites in biological samples. During the past decade, metabolomic techniques have yielded novel and potentially important findings, revealing insight into metabolic perturbations associated with these diseases. This chapter provides metabolomic methodologies describing a nuclear magnetic resonance (NMR)-based non-targeted approach that has been utilized to make important contributions toward a better understanding of IBD.
authors.affiliation: Department of Medicine, The University of Colorado Anschutz Medical Campus, Aurora, CO, USA.|Mucosal Inflammation Program, The University of Colorado Anschutz Medical Campus, Aurora, CO, USA.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Kao
authors.affiliation: Department of Medicine, The University of Colorado Anschutz Medical Campus, Aurora, CO, USA.|Mucosal Inflammation Program, The University of Colorado Anschutz Medical Campus, Aurora, CO, USA.
authors.forename: Jordi M
authors.initials: JM
authors.lastname: Lanis
authors.affiliation: Department of Medicine, The University of Colorado Anschutz Medical Campus, Aurora, CO, USA.|Mucosal Inflammation Program, The University of Colorado Anschutz Medical Campus, Aurora, CO, USA.
authors.forename: Erica
authors.initials: E
authors.lastname: Alexeev
authors.affiliation: Department of Microbiology and Immunology, Montana State University, 109 Lewis Hall, Bozeman, MT, 59717, USA. douglas.kominsky@montana.edu.
authors.forename: Douglas J
authors.initials: DJ
authors.lastname: Kominsky
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D003106:Colon / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000378:metabolism; D008279:Magnetic Resonance Imaging / Q000379:methods*; D055432:Metabolomics / Q000379:methods*; D051379:Mice
keywords: Inflammatory bowel disease; Intestinal epithelium; Metabolomics; Mucosa; Nuclear magnetic resonance