title: Localization of Acrolein-Lysine Adduct in Fibrovascular Tissues of Proliferative Diabetic Retinopathy.
abstract: To determine the presence of Nε-(3-formyl-3,4-dehydropiperidino) lysine adduct (FDP-Lys), unsaturated aldehyde acrolein-derived lipoxidation end-product, in fibrovascular tissues obtained from patients with proliferative diabetic retinopathy (PDR).
  
  
  
  Fibrovascular tissues were collected from 11 eyes of 11 patients with PDR and paraffin-embedded tissue sections were prepared. Tissue localization of FDP-Lys was studied by immunohistochemistry. Signal intensity was quantified by two masked evaluators and graded into three discrete categories. The relationship between FDP-Lys staining and vascular density was analyzed. In addition, subcellular localization of FDP-Lys was studied by immunofluorescent microscopy. The impact of acrolein on cell viability and proliferation was assessed and the expression level of heme oxygenase-1 (HO-1) mRNA was quantified by real-time polymerase chain reaction (PCR) in cultured retinal microvascular endothelial cells.
  
  
  
  In fibrovascular tissues, FDP-Lys staining was found in vascular components containing CD34-positive cells and alpha smooth muscle actin (α-SMA)-positive cells, and clusters of rabbit anti-glial fibrillary acid protein (GFAP)-positive cells. Immunofluorescent staining depicted subcellular localization of FDP-Lys in the nucleus and cytoplasm of the cells. Morphological analysis revealed that fibrovascular tissues with FDP-Lys staining in vascular components showed high vascular density. Exposure of cultured endothelial cells to high concentration of acrolein resulted in the decrease of cell viability and proliferation, whereas lower concentration of acrolein increased cell viability and proliferation. Sublethal concentration of acrolein upregulated HO-1 mRNA expression in retinal microvascular endothelial cells.
  
  
  
  The current data demonstrated the presence of FDP-Lys in fibrovascular tissues and indicate its involvement in fibrovascular proliferation in PDR.
authors.affiliation: a Laboratory of Ocular Cell Biology and Visual Science , Hokkaido University Graduate School of Medicine , Sapporo , Japan.|b Department of Ophthalmology , Hokkaido University Graduate School of Medicine , Sapporo , Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Dong
authors.affiliation: a Laboratory of Ocular Cell Biology and Visual Science , Hokkaido University Graduate School of Medicine , Sapporo , Japan.|b Department of Ophthalmology , Hokkaido University Graduate School of Medicine , Sapporo , Japan.
authors.forename: Kousuke
authors.initials: K
authors.lastname: Noda
authors.affiliation: a Laboratory of Ocular Cell Biology and Visual Science , Hokkaido University Graduate School of Medicine , Sapporo , Japan.|b Department of Ophthalmology , Hokkaido University Graduate School of Medicine , Sapporo , Japan.
authors.forename: Miyuki
authors.initials: M
authors.lastname: Murata
authors.affiliation: a Laboratory of Ocular Cell Biology and Visual Science , Hokkaido University Graduate School of Medicine , Sapporo , Japan.|b Department of Ophthalmology , Hokkaido University Graduate School of Medicine , Sapporo , Japan.
authors.forename: Shiho
authors.initials: S
authors.lastname: Yoshida
authors.affiliation: b Department of Ophthalmology , Hokkaido University Graduate School of Medicine , Sapporo , Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Saito
authors.affiliation: a Laboratory of Ocular Cell Biology and Visual Science , Hokkaido University Graduate School of Medicine , Sapporo , Japan.|b Department of Ophthalmology , Hokkaido University Graduate School of Medicine , Sapporo , Japan.
authors.forename: Atsuhiro
authors.initials: A
authors.lastname: Kanda
authors.affiliation: a Laboratory of Ocular Cell Biology and Visual Science , Hokkaido University Graduate School of Medicine , Sapporo , Japan.|b Department of Ophthalmology , Hokkaido University Graduate School of Medicine , Sapporo , Japan.
authors.forename: Susumu
authors.initials: S
authors.lastname: Ishida
mesh_terms: D000171:Acrolein / Q000494:pharmacology; D000199:Actins / Q000378:metabolism; D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003930:Diabetic Retinopathy / Q000378:metabolism* / Q000473:pathology; D004730:Endothelium, Vascular / Q000378:metabolism*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D051547:Heme Oxygenase-1 / Q000235:genetics; D006801:Humans; D008239:Lysine / Q000031:analogs & derivatives* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D016612:Paraffin Embedding; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D015861:Retinal Neovascularization / Q000378:metabolism* / Q000473:pathology; D012171:Retinal Vessels / Q000378:metabolism* / Q000473:pathology; D055815:Young Adult
chemical_list: C541116:ACTA2 protein, human; D000199:Actins; D018952:Antigens, CD34; D005904:Glial Fibrillary Acidic Protein; C113380:N(epsilon)-(3-formyl-3,4-dihydropiperidino)lysine; D012333:RNA, Messenger; D000171:Acrolein; D051547:Heme Oxygenase-1; D008239:Lysine
keywords: Acrolein; Acrolein-Lys Adduct; FDP-Lys; diabetic retinopathy; fibrovascular tissues