title: Reduced stability and intracellular transport of dsRNA contribute to poor RNAi response in lepidopteran insects.
abstract: RNA interference (RNAi) has become a widely used reverse genetic tool to study gene function in eukaryotic organisms and is being developed as a technology for insect pest management. The efficiency of RNAi varies among organisms. Insects from different orders also display differential efficiency of RNAi, ranging from highly efficient (coleopterans) to very low efficient (lepidopterans). We investigated the reasons for varying RNAi efficiency between lepidopteran and coleopteran cell lines and also between the Colorado potato beetle, Leptinotarsa decemlineata and tobacco budworm, Heliothis virescens. The dsRNA either injected or fed was degraded faster in H. virescens than in L. decemlineata. Both lepidopteran and coleopteran cell lines and tissues efficiently took up the dsRNA. Interestingly, the dsRNA administered to coleopteran cell lines and tissues was taken up and processed to siRNA whereas the dsRNA was taken up by lepidopteran cell lines and tissues but no siRNA was detected in the total RNA isolated from these cell lines and tissues. The data included in this paper showed that the degradation and intracellular transport of dsRNA are the major factors responsible for reduced RNAi efficiency in lepidopteran insects.
authors.affiliation: a Department of Entomology , College of Agriculture, Food and Environment, Agriculture Science Center North, University of Kentucky , Lexington , KY , USA.
authors.forename: Jayendra Nath
authors.initials: JN
authors.lastname: Shukla
authors.affiliation: a Department of Entomology , College of Agriculture, Food and Environment, Agriculture Science Center North, University of Kentucky , Lexington , KY , USA.
authors.forename: Megha
authors.initials: M
authors.lastname: Kalsi
authors.affiliation: b Agricultural Biotechnology Research and Development, DuPont Pioneer , Johnston , IA , USA.
authors.forename: Amit
authors.initials: A
authors.lastname: Sethi
authors.affiliation: c DowAgrosciences LLC , Indianapolis , Indiana , USA.
authors.forename: Kenneth E
authors.initials: KE
authors.lastname: Narva
authors.affiliation: c DowAgrosciences LLC , Indianapolis , Indiana , USA.
authors.forename: Elane
authors.initials: E
authors.lastname: Fishilevich
authors.affiliation: a Department of Entomology , College of Agriculture, Food and Environment, Agriculture Science Center North, University of Kentucky , Lexington , KY , USA.
authors.forename: Satnam
authors.initials: S
authors.lastname: Singh
authors.affiliation: a Department of Entomology , College of Agriculture, Food and Environment, Agriculture Science Center North, University of Kentucky , Lexington , KY , USA.
authors.forename: Kanakachari
authors.initials: K
authors.lastname: Mogilicherla
authors.affiliation: a Department of Entomology , College of Agriculture, Food and Environment, Agriculture Science Center North, University of Kentucky , Lexington , KY , USA.
authors.forename: Subba Reddy
authors.initials: SR
authors.lastname: Palli
mesh_terms: D000818:Animals; D001693:Biological Transport, Active / Q000502:physiology; D001517:Coleoptera; D034622:RNA Interference / Q000502:physiology*; D012330:RNA, Double-Stranded / Q000235:genetics / Q000378:metabolism*; D061987:Sf9 Cells; D018411:Spodoptera
chemical_list: D012330:RNA, Double-Stranded
keywords: Coleoptera; RNA interference; double-stranded RNA; lepidoptera