title: Identification of Secreted Exoproteome Fingerprints of Highly-Virulent and Non-Virulent Staphylococcus aureus Strains.
abstract: Staphylococcus aureus is a commensal inhabitant of skin and mucous membranes in nose vestibule but also an important opportunistic pathogen of humans and livestock. The extracellular proteome as a whole constitutes its major virulence determinant; however, the involvement of particular proteins is still relatively poorly understood. In this study, we compared the extracellular proteomes of poultry-derived S. aureus strains exhibiting a virulent (VIR) and non-virulent (NVIR) phenotype in a chicken embryo experimental infection model with the aim to identify proteomic signatures associated with the particular phenotypes. Despite significant heterogeneity within the analyzed proteomes, we identified alpha-haemolysin and bifunctional autolysin as indicators of virulence, whereas glutamylendopeptidase production was characteristic for non-virulent strains. Staphopain C (StpC) was identified in both the VIR and NVIR proteomes and the latter fact contradicted previous findings suggesting its involvement in virulence. By supplementing NVIR, StpC-negative strains with StpC, and comparing the virulence of parental and supplemented strains, we demonstrated that staphopain C alone does not affect staphylococcal virulence in a chicken embryo model.
authors.affiliation: Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University Krakow, Poland.
authors.forename: Emilia
authors.initials: E
authors.lastname: Bonar
authors.affiliation: Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University Krakow, Poland.
authors.forename: Iwona
authors.initials: I
authors.lastname: Wojcik
authors.affiliation: Malopolska Centre of Biotechnology, Jagiellonian University Krakow, Poland.
authors.forename: Urszula
authors.initials: U
authors.lastname: Jankowska
authors.affiliation: Malopolska Centre of Biotechnology, Jagiellonian UniversityKrakow, Poland; Department of Physical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian UniversityKrakow, Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Kedracka-Krok
authors.affiliation: Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University Krakow, Poland.
authors.forename: Michal
authors.initials: M
authors.lastname: Bukowski
authors.affiliation: Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University Krakow, Poland.
authors.forename: Klaudia
authors.initials: K
authors.lastname: Polakowska
authors.affiliation: Department of Veterinary and Animal Reproduction and Welfare, Faculty of Animal Welfare, University of Agriculture in Krakow Krakow, Poland.
authors.forename: Marcin W
authors.initials: MW
authors.lastname: Lis
authors.affiliation: Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University Krakow, Poland.
authors.forename: Maja
authors.initials: M
authors.lastname: Kosecka-Strojek
authors.affiliation: Department of Medical Microbiology, University of Groningen, University Medical Center Groningen Groningen, Netherlands.
authors.forename: Artur J
authors.initials: AJ
authors.lastname: Sabat
authors.affiliation: Malopolska Centre of Biotechnology, Jagiellonian UniversityKrakow, Poland; Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian UniversityKrakow, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Dubin
authors.affiliation: Department of Medical Microbiology, University of Groningen, University Medical Center Groningen Groningen, Netherlands.
authors.forename: Alexander W
authors.initials: AW
authors.lastname: Friedrich
authors.affiliation: Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University Krakow, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Miedzobrodzki
authors.affiliation: Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University Krakow, Poland.
authors.forename: Adam
authors.initials: A
authors.lastname: Dubin
authors.affiliation: Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University Krakow, Poland.
authors.forename: Benedykt
authors.initials: B
authors.lastname: Wladyka
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis* / Q000378:metabolism*; D002642:Chick Embryo; D004195:Disease Models, Animal; D020543:Proteome / Q000032:analysis*; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000737:chemistry* / Q000254:growth & development / Q000472:pathogenicity; D037521:Virulence Factors / Q000032:analysis*
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; D037521:Virulence Factors
keywords: Staphylococcus; chicken embryo model; pathogen; protein; proteomics; virulence