title: Diquafosol Ophthalmic Solution Increases Pre- and Postlens Tear Film During Contact Lens Wear in Rabbit Eyes.
abstract: OBJECTIVE
  To investigate the behavior of prelens tear film (PLTF) and postlens tear film (PoLTF) after the instillation of diquafosol using an experimental rabbit model of eyes with contact lens.
  
  
  METHODS
  Cross-sectional, anterior segment optical coherence tomographic images of the inferior midperipheral cornea were obtained at baseline and at 5, 15, 30, 60, 90, and 120 min after the instillation of 3% diquafosol ophthalmic solution in 10 Japanese white rabbits wearing contact lenses. From the obtained images, the areas of the PLTF and PoLTF were calculated. Both artificial tear solution and 0.1% sodium hyaluronate ophthalmic solution were used for comparison.
  
  
  RESULTS
  Significant fluid accumulation in both the PLTF and PoLTF was observed after diquafosol instillation, whereas no fluid accumulation was visible after the instillation of artificial tear or sodium hyaluronate. The increase in PLTF area after diquafosol instillation was significantly higher (P<0.01) at 15 and 30 min than that after the instillation of artificial tear or sodium hyaluronate. The increase in PoLTF area up to 60 min after the instillation of diquafosol was significantly higher (P<0.01) than that after the instillation of either of the other two drugs.
  
  
  CONCLUSIONS
  Instillation of 3% diquafosol ophthalmic solution increases PLTF and PoLTF in rabbit eyes with contact lenses. Diquafosol has potential as a treatment option for contact lens-related dry eye.
authors.affiliation: Department of Ophthalmology (Y.N., S.K., K.N., H.W.), Osaka University Graduate School of Medicine, Suita, Osaka, Japan; Nara Research and Development Center (Y.O., T.N., H.M.), Santen Pharmaceutical Co., Ltd, Ikoma, Nara, Japan; and Department of Ophthalmology (H.W.), Kansai Rosai Hospital, Amagasaki, Hyogo, Japan.
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Nagahara
authors.forename: Shizuka
authors.initials: S
authors.lastname: Koh
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Oshita
authors.forename: Takashi
authors.initials: T
authors.lastname: Nagano
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Mano
authors.forename: Kohji
authors.initials: K
authors.lastname: Nishida
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Watanabe
mesh_terms: D000818:Animals; D003263:Contact Lenses, Hydrophilic / Q000009:adverse effects; D003315:Cornea / Q000187:drug effects*; D004195:Disease Models, Animal; D015352:Dry Eye Syndromes / Q000188:drug therapy; D009883:Ophthalmic Solutions / Q000494:pharmacology*; D011122:Polyphosphates / Q000494:pharmacology*; D011817:Rabbits; D013666:Tears / Q000187:drug effects* / Q000378:metabolism; D041623:Tomography, Optical Coherence; D014500:Uracil Nucleotides / Q000494:pharmacology*
chemical_list: D009883:Ophthalmic Solutions; D011122:Polyphosphates; D014500:Uracil Nucleotides; C403315:diquafosol