title: Effects of body temperature on post-anoxic oxidative stress from the perspective of postnatal physiological adaptive processes in rats.
abstract: It is well known that decrease in body temperature provides protection to newborns subjected to anoxia/ischemia. We hypothesized that the normal body temperature of 33°C in neonatal rats (4°C below normal body temperature in adults) is in fact a preadaptation to protect CNS from anoxia and further reductions as well as elevations in temperature may be counterproductive. Our experiments aimed to examine the effect of changes in body temperature on oxidative stress development in newborn rats exposed to anoxia. Two-day-old Wistar rats were divided into 4 temperature groups: i. hypothermic at body temperature of 31°C, ii. maintaining physiological neonatal body temperature of 33°C, iii. forced to maintain hyperthermic temperature of 37°C, and i.v. forced to maintain hyperthermic temperature of 39°C. The temperature was controlled starting 15 minutes before and afterword during 10 minutes of anoxia as well as for 2 hours post-anoxia. Cerebral concentrations of lipid peroxidation products malondialdehyde (MDA) and conjugated dienes (CD) and the activities of antioxidant enzymes had been determined post mortem: immediately after anoxia was finished and 3, 7, and 14 days later. There were no post-anoxic changes in the concentration of MDA, CD and in antioxidant enzymes activity in newborn rats kept at their physiological body temperature of 33°C. In contrast, perinatal anoxia at body temperature elevated to 37°C or 39°C as well as under hypothermic conditions (31°C) intensified post-anoxic oxidative stress and depleted the antioxidant pool. Overall, these findings suggest that elevated body temperature (hyperthermia or fever), as well as exceeding cooling beyond the physiological level of body temperature of newborn rats, may extend perinatal anoxia-induced brain lesions. Our findings provide new insights into the role of body temperature in anoxic insult in vivo.
authors.affiliation: N. Copernicus University, Faculty of Biology and Environmental Protection, Department of Animal Physiology, Torun, Poland.
authors.forename: H
authors.initials: H
authors.lastname: Kletkiewicz
authors.affiliation: N. Copernicus University, Faculty of Biology and Environmental Protection, Department of Animal Physiology, Torun, Poland. rogal@umk.pl.
authors.forename: J
authors.initials: J
authors.lastname: Rogalska
authors.affiliation: N. Copernicus University, Faculty of Biology and Environmental Protection, Department of Animal Physiology, Torun, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Nowakowska
authors.affiliation: N. Copernicus University, The Chair of Medical Biology, Collegium Medicum, Bydgoszcz, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Wozniak
authors.affiliation: N. Copernicus University, The Chair of Medical Biology, Collegium Medicum, Bydgoszcz, Poland.
authors.forename: C
authors.initials: C
authors.lastname: Mila-Kierzenkowska
authors.affiliation: N. Copernicus University, Faculty of Biology and Environmental Protection, Department of Animal Physiology, Torun, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Caputa
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D000831:Animals, Newborn; D001831:Body Temperature; D001921:Brain / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D000860:Hypoxia / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase