title: Sodium Selenite Protects Against Silver Nanoparticle-Induced Testicular Toxicity and Inflammation.
abstract: Metal nanomaterials hold great potential and play an important role in consumer products. However, the increasing use of nanomaterials has raised concern over inadvertent exposure and potential risks for human health and the environment. Henceforth, in vivo testing of nanoparticles and protection against its toxicity is required. Using rat as an animal model, effect of sodium selenite (Se), an essential trace element, on rat testes exposed to silver nanoparticles (AgNPs) was evaluated. Male rats were treated with AgNPs (5 mg/kg/b.w) i/p or Se (0.2 mg/kg/b.w) by gavage. AgNP administration decreased Glutathione (GSH) levels and activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) and increased levels of malondialdehyde (MDA) and expression of interleukin-1 beta (IL-1β), IL-6, and tumor necrosis factor alpha (TNF-α). However, treatment with Se increased GSH levels and activities of SOD, CAT, and GPx compared with AgNP-treated group and decreased the level of MDA and inflammatory biomarkers significantly (p < 0.05) as compared with AgNP-treated group. Light microscopic analyses also revealed that AgNP induced histopathological changes in testes tissue. Further, protection by Se on biochemical results was confirmed by alleviation of the histopathological changes in the tissue. Results show the adverse effects of AgNPs on the male reproductive tract, particularly spermatogenesis, and suggest that Se possesses significant potential in reducing AgNP-induced testicular toxicity.
authors.affiliation: Clinical Laboratory Sciences, Applied Medical Science, King Saud University, Riyadh, Saudi Arabia. sansar@ksu.edu.sa.
authors.forename: Sabah
authors.initials: S
authors.lastname: Ansar
authors.affiliation: Clinical Laboratory Sciences, Applied Medical Science, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Manal
authors.initials: M
authors.lastname: Abudawood
authors.affiliation: Zoology Department, College of Science, King Saud University, Riyadh, Saudi Arabia.|Zoology Department, Faculty of Science, University of Alexandria, Moharram Bey, Alexandria, Egypt.
authors.forename: Sherifa Shaker
authors.initials: SS
authors.lastname: Hamed
authors.affiliation: Chemistry and Biochemistry Department, University of California, Santa Cruz, CA, USA.
authors.forename: Mukhtar M
authors.initials: MM
authors.lastname: Aleem
mesh_terms: D000818:Animals; D016207:Cytokines / Q000378:metabolism; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D010088:Oxidoreductases / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012834:Silver / Q000737:chemistry / Q000633:toxicity*; D018038:Sodium Selenite / Q000494:pharmacology*; D013737:Testis / Q000378:metabolism* / Q000473:pathology
chemical_list: D016207:Cytokines; D012834:Silver; D008315:Malondialdehyde; D010088:Oxidoreductases; D018038:Sodium Selenite
keywords: Inflammation; Selenium; Silver nanoparticles; Testes; Toxicity