title: Molecular Epidemiology of Chikungunya Virus by Sequencing.
abstract: Molecular surveillance of Chikungunya virus (CHIKV) is important as it provides data on the circulating CHIKV genotypes in endemic countries and enabling activation of measures to be taken in the event of a pending outbreak. Molecular surveillance is carried out by first detecting CHIKV in susceptible humans or among field-caught mosquitoes. This is followed by sequencing a selected region of the virus which will provide evidence on the source of the virus and possible association of the virus to increased cases of Chikungunya infections.
authors.affiliation: Virology Unit, Infectious Diseases Research Centre, Institute for Medical Research, Jalan Pahang, Kuala Lumpur, 50588, Malaysia. rthayan@gmail.com.
authors.forename: Ravindran
authors.initials: R
authors.lastname: Thayan
authors.affiliation: Virology Unit, Infectious Diseases Research Centre, Institute for Medical Research, Jalan Pahang, Kuala Lumpur, 50588, Malaysia.
authors.forename: Mohd Apandi
authors.initials: MA
authors.lastname: Yusof
authors.affiliation: Virology Unit, Infectious Diseases Research Centre, Institute for Medical Research, Jalan Pahang, Kuala Lumpur, 50588, Malaysia.
authors.forename: Zainah
authors.initials: Z
authors.lastname: Saat
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Jalan Universiti, Kuala Lumpur, 50603, Malaysia.
authors.forename: Shamala Devi
authors.initials: SD
authors.lastname: Sekaran
authors.affiliation: Faculty of Medicine, Jalan Hospital, Universiti Teknologi MARA (UiTM), Sungai Buloh Campus, 47000 Sungai Buloh, Selangor Darul Ehsan, Malaysia.
authors.forename: Seok Mui
authors.initials: SM
authors.lastname: Wang
mesh_terms: D000818:Animals; D002460:Cell Line; D065632:Chikungunya Fever / Q000175:diagnosis* / Q000453:epidemiology*; D002646:Chikungunya virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D009033:Culicidae / Q000821:virology*; D004196:Disease Outbreaks; D006801:Humans; D017720:Molecular Epidemiology; D010802:Phylogeny; D011159:Population Surveillance; D017423:Sequence Analysis, RNA; D014759:Viral Envelope Proteins / Q000235:genetics
chemical_list: D014759:Viral Envelope Proteins
keywords: Molecular surveillance; Phylogenetic tree; RT-PCR; Sequencing