title: Sevoflurane anesthetic preconditioning protects the lung endothelial glycocalyx from ischemia reperfusion injury in an experimental lung autotransplant model.
abstract: The glycocalyx is a glycoprotein-polysaccaride layer covering the endothelium luminal surface, and plays a key regulatory role in several endothelial functions. Lung ischemia reperfusion (IR) is a clinical entity that occurs in everyday thoracic surgery and causes glycocalix destruction and a florid local and systemic immune response. Moreover, sevoflurane is able to modulate the inflammatory response triggered by IR lung injury. In this study, we evaluated the protective effects of sevoflurane on the pulmonary endothelial glycocalyx in an in-vivo lung autotransplant model in pigs.
  
  
  
  Sixteen Large White pigs underwent pneumonectomy plus lung autotransplant. They were divided into two groups depending on the hypnotic agent received (propofol or anesthetic preconditioning with sevoflurane). Glycocalyx components (syndecan-1 and heparan sulphate), cathepsin B, chemokines (MCP-1, MIP-1, and MIP-2) and adhesion molecules (VCAM and ICAM-1) were measured at four different timepoints using porcine-specific enzyme-linked immunosorbent assay (ELISA) kits.
  
  
  
  There were no differences between groups in weight or in surgical and one-lung ventilation time. Greater glycocalyx destruction and higher chemokine and adhesion molecule expression were observed in the group that did not receive sevoflurane. Heparan sulphate and serum syndecan levels were higher in the propofol group (P < 0.0001) after reperfusion, as was cathepsin B activity (P < 0.015). MCP-1, MIP-1, MIP-2, VCAM, and ICAM-1 levels were also higher in the propofol group (P < 0.006).
  
  
  
  Sevoflurane preconditioning protects pulmonary glycocalyx and reduces expression of leukocyte chemokines in an in-vivo model of pulmonary IR.
authors.affiliation: Anesthesiologist in cardiothoracic section, Anesthesiology Department, Gregorio Marañon Hospital, Doctor Esquerdo Street 46, 28007, Madrid, Spain. kassss1@yahoo.es.
authors.forename: Javier
authors.initials: J
authors.lastname: Casanova
authors.affiliation: Thoracic Surgery Department, Gregorio Marañon Hospital, Madrid, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Simon
authors.affiliation: Biochemistry Department, Complutense University, Madrid, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Vara
authors.affiliation: Anesthesiologist in cardiothoracic section, Anesthesiology Department, Gregorio Marañon Hospital, Doctor Esquerdo Street 46, 28007, Madrid, Spain.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Sanchez
authors.affiliation: Biochemistry Department, Complutense University, Madrid, Spain.
authors.forename: Lisa
authors.initials: L
authors.lastname: Rancan
authors.affiliation: Anesthesiologist in cardiothoracic section, Anesthesiology Department, Gregorio Marañon Hospital, Doctor Esquerdo Street 46, 28007, Madrid, Spain.
authors.forename: Selma
authors.initials: S
authors.lastname: Abubakra
authors.affiliation: Biochemistry Department, Complutense University, Madrid, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Calvo
authors.affiliation: Anesthesiologist in cardiothoracic section, Anesthesiology Department, Gregorio Marañon Hospital, Doctor Esquerdo Street 46, 28007, Madrid, Spain.
authors.forename: Francisco Jose
authors.initials: FJ
authors.lastname: Gonzalez
authors.affiliation: Anesthesiologist in cardiothoracic section, Anesthesiology Department, Gregorio Marañon Hospital, Doctor Esquerdo Street 46, 28007, Madrid, Spain.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Garutti
mesh_terms: D000777:Anesthetics / Q000008:administration & dosage* / Q000494:pharmacology; D000818:Animals; D019276:Glycocalyx / Q000187:drug effects*; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008738:Methyl Ethers / Q000008:administration & dosage*; D061810:One-Lung Ventilation; D011013:Pneumonectomy; D015742:Propofol / Q000494:pharmacology; D015427:Reperfusion Injury / Q000503:physiopathology*; D000077149:Sevoflurane; D013552:Swine; D014182:Transplantation, Autologous
chemical_list: D000777:Anesthetics; D008738:Methyl Ethers; D018799:Intercellular Adhesion Molecule-1; D000077149:Sevoflurane; D015742:Propofol
keywords: Anesthetic preconditioning; Glycocalyx; Lung transplantation; Reperfusion injury; Sevoflurane