title: Pig epidemic diarrhoea virus S gene variant with a large deletion non-lethal to colostrum-deprived newborn piglets.
abstract: We previously identified a third porcine epidemic diarrhoea virus (PEDV) S variant with a large deletion of 582 nucleotides in the 5' terminal region of the S gene, in addition to the North American type and the S INDELs type. To investigate the pathogenicity of this variant, TTR-2/JPN/2014, we performed experimental infection using colostrum-deprived piglets and compared the results with those from the North American type PEDV, OKN-1/JPN/2013. Fifteen newborn piglets were divided into two groups of 7-8 piglets each and inoculated orally with one of PEDV isolates maintained at the eighth passage in Vero cell culture. Although all PEDV-inoculated piglets showed acute watery diarrhoea, lethality clearly differed between both PEDV-inoculated groups. Moreover, there were differences in virus distribution and lesions on the intestines between the two PEDV-inoculated groups. Therefore, our data suggest that the OKN-1/JPN/2013 PEDV isolate is virulent, whereas the TTR-2/JPN/2014 PEDV isolate is avirulent.
authors.affiliation: Viral Disease and Epidemiology Research Division, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Tsukuba, Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Suzuki
authors.affiliation: Pathology and Pathophysiology Research Division, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Tsukuba, Japan.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Shibahara
authors.affiliation: Institute of Animal Health, National Federation of Agricultural Co-operative Associations, Sakura, Japan.
authors.forename: Ryosaku
authors.initials: R
authors.lastname: Yamaguchi
authors.affiliation: Oita Livestock Hygiene Service Center, Oita, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Nakade
authors.affiliation: Viral Disease and Epidemiology Research Division, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Tsukuba, Japan.
authors.forename: Takehisa
authors.initials: T
authors.lastname: Yamamoto
authors.affiliation: Viral Disease and Epidemiology Research Division, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Tsukuba, Japan.
authors.forename: Ayako
authors.initials: A
authors.lastname: Miyazaki
authors.affiliation: Viral Disease and Epidemiology Research Division, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Tsukuba, Japan.
authors.forename: Seiichi
authors.initials: S
authors.lastname: Ohashi
mesh_terms: D032761:Animal Experimentation; D000818:Animals; D000831:Animals, Newborn; D003126:Colostrum; D018352:Coronavirus Infections / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D003967:Diarrhea / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D053485:Porcine epidemic diarrhea virus / Q000235:genetics* / Q000472:pathogenicity*; D017384:Sequence Deletion; D064370:Spike Glycoprotein, Coronavirus / Q000235:genetics*; D016019:Survival Analysis; D013552:Swine; D013553:Swine Diseases / Q000473:pathology* / Q000821:virology
chemical_list: D064370:Spike Glycoprotein, Coronavirus