title: Enzymatic Process for High-Yield Turanose Production and Its Potential Property as an Adipogenesis Regulator.
abstract: Turanose is a sucrose isomer naturally existing in honey and a promising functional sweetener due to its low glycemic response. In this study, the extrinsic fructose effect on turanose productivity was examined in Neisseria amylosucrase reaction. Turanose was produced, by increasing the amount of extrinsic fructose as a reaction modulator, with high concentration of sucrose substrate, which resulted in 73.7% of production yield. In physiological functionality test, lipid accumulation in 3T3-L1 preadipocytes in the presence of high amounts of pure glucose was attenuated by turanose substitution in a dose-dependent manner. Turanose treatments at concentrations representing 50%, 75%, and 100% of total glucose concentration in cell media significantly reduced lipid accumulation by 18%, 35%, and 72%, respectively, as compared to controls. This result suggested that turanose had a positive role in controlling adipogenesis, and enzymatic process of turanose production has a potential to develop a functional food ingredient for controlling obesity and related chronic diseases.
authors.affiliation: Department of Food Science and Technology, and Carbohydrate Bioproduct Research Center, Sejong University , Gunja-Dong, Gwangjin-Gu, Seoul 143-747, Republic of Korea.
authors.forename: Min-Oh
authors.initials: MO
authors.lastname: Park
authors.affiliation: Department of Food Science and Biotechnology, College of BioNano Technology, Gachon University , Seongnam, Gyeonggi-do 461-701, Republic of Korea.
authors.forename: Byung-Hoo
authors.initials: BH
authors.lastname: Lee
authors.affiliation: Department of Nutritional Science and Food Management, Ewha Womans University , Seoul 120-750, Republic of Korea.
authors.forename: Eunjin
authors.initials: E
authors.lastname: Lim
authors.affiliation: Department of Nutritional Science and Food Management, Ewha Womans University , Seoul 120-750, Republic of Korea.
authors.forename: Ji Ye
authors.initials: JY
authors.lastname: Lim
authors.affiliation: Department of Nutritional Science and Food Management, Ewha Womans University , Seoul 120-750, Republic of Korea.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Graduate School of Biotechnology, and Institute of Life Science and Resources, Kyung Hee University , Seocheon, Kiheung, Yongin 446-701, Republic of Korea.
authors.forename: Cheon-Seok
authors.initials: CS
authors.lastname: Park
authors.affiliation: Department of Food and Nutrition, Hanyang University , 17 Haengdang-dong, Seongdong-gu, Seoul 133-791, Republic of Korea.
authors.forename: Hyeon Gyu
authors.initials: HG
authors.lastname: Lee
authors.affiliation: Department of Food Science and Technology, and Carbohydrate Bioproduct Research Center, Sejong University , Gunja-Dong, Gwangjin-Gu, Seoul 143-747, Republic of Korea.
authors.forename: Hee-Kwon
authors.initials: HK
authors.lastname: Kang
authors.affiliation: Department of Food Science and Technology, and Carbohydrate Bioproduct Research Center, Sejong University , Gunja-Dong, Gwangjin-Gu, Seoul 143-747, Republic of Korea.
authors.forename: Sang-Ho
authors.initials: SH
authors.lastname: Yoo
mesh_terms: D041721:3T3-L1 Cells / Q000187:drug effects / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D001709:Biotechnology / Q000379:methods; D004187:Disaccharides / Q000096:biosynthesis* / Q000378:metabolism / Q000494:pharmacology*; D005632:Fructose / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D005964:Glucosyltransferases / Q000737:chemistry / Q000378:metabolism*; D050356:Lipid Metabolism / Q000187:drug effects; D051379:Mice; D047495:PPAR gamma / Q000235:genetics; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics; D013395:Sucrose / Q000378:metabolism; D019014:fas Receptor / Q000235:genetics
chemical_list: D004187:Disaccharides; C491899:Fas protein, mouse; D047495:PPAR gamma; C497388:Srebf1 protein, mouse; D051780:Sterol Regulatory Element Binding Protein 1; D019014:fas Receptor; D005632:Fructose; D013395:Sucrose; C029454:turanose; D005964:Glucosyltransferases; C019700:amylosucrase
keywords: Neisseria polysaccharea; adipogenesis; amylosucrase; fructose; turanose