title: Middle age has a significant impact on gene expression during skin wound healing in male mice.
abstract: The vast majority of research on the impact of age on skin wound healing (WH) compares old animals to young ones. The middle age is often ignored in biogerontological research despite the fact that many functions that decline in an age-dependent manner have starting points in mid-life. With this in mind, we examined gene expression patterns during skin WH in late middle-aged versus young adult male mice, using the head and back punch models. The rationale behind this study was that the impact of age would first be detectable at the transcriptional level. We pinpointed several pathways which were over-activated in the middle-aged mice, both in the intact skin and during WH. Among them were various metabolic, immune-inflammatory and growth-promoting pathways. These transcriptional changes were much more pronounced in the head than in the back. In summary, the middle age has a significant impact on gene expression in intact and healing skin. It seems that the head punch model is more sensitive to the effect of age than the back model, and we suggest that it should be more widely applied in aging research on wound healing.
authors.affiliation: The Shraga Segal Department of Microbiology, Immunology and Genetics, Center for Multidisciplinary Research on Aging, Ben-Gurion University of the Negev, POB 653, 8410501, Beer-Sheva, Israel.
authors.forename: Hagai
authors.initials: H
authors.lastname: Yanai
authors.affiliation: Division of Plastic and Reconstructive Surgery, Department of Surgery, Medical University of Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austria. david.lumenta@gmail.com.|Division of Plastic, Aesthetic and Reconstructive Surgery, Department of Surgery, Medical University of Graz, Auenbruggerplatz 29, 8036, Graz, Austria. david.lumenta@gmail.com.
authors.forename: David Benjamin
authors.initials: DB
authors.lastname: Lumenta
authors.affiliation: AIT-Austrian Institute of Technology, Donau-City-Straße 1, 1220, Vienna, Austria.
authors.forename: Klemens
authors.initials: K
authors.lastname: Vierlinger
authors.affiliation: AIT-Austrian Institute of Technology, Donau-City-Straße 1, 1220, Vienna, Austria.
authors.forename: Manuela
authors.initials: M
authors.lastname: Hofner
authors.affiliation: Division of Plastic and Reconstructive Surgery, Department of Surgery, Medical University of Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austria.
authors.forename: Hugo-Benito
authors.initials: HB
authors.lastname: Kitzinger
authors.affiliation: Division of Plastic, Aesthetic and Reconstructive Surgery, Department of Surgery, Medical University of Graz, Auenbruggerplatz 29, 8036, Graz, Austria.
authors.forename: Lars-Peter
authors.initials: LP
authors.lastname: Kamolz
authors.affiliation: AIT-Austrian Institute of Technology, Donau-City-Straße 1, 1220, Vienna, Austria.
authors.forename: Christa
authors.initials: C
authors.lastname: Nöhammer
authors.affiliation: Department of Pathology, University of Verona, Piazzale A. Scuro 10, 37134, Verona, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Chilosi
authors.affiliation: The Shraga Segal Department of Microbiology, Immunology and Genetics, Center for Multidisciplinary Research on Aging, Ben-Gurion University of the Negev, POB 653, 8410501, Beer-Sheva, Israel.
authors.forename: Vadim E
authors.initials: VE
authors.lastname: Fraifeld
mesh_terms: D000375:Aging / Q000378:metabolism* / Q000502:physiology*; D000818:Animals; D016207:Cytokines / Q000378:metabolism; D005786:Gene Expression Regulation; D058847:Inflammasomes / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D022125:Lacerations / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012727:Sex Characteristics; D012867:Skin / Q000293:injuries* / Q000378:metabolism* / Q000473:pathology; D014945:Wound Healing / Q000502:physiology*
chemical_list: D016207:Cytokines; D058847:Inflammasomes; D036341:Intercellular Signaling Peptides and Proteins
keywords: Aging; Mice; Middle-age; Skin; Wound healing