title: Sucrose ester based cationic liposomes as effective non-viral gene vectors for gene delivery.
abstract: As sucrose esters (SEs) are natural and biodegradable excipients with excellent drug dissolution and drug absorption/permeation in controlled release systems, we firstly incorporated SE into liposomes for gene delivery in this article. A peptide-based lipid (CDO14), Gemini-based quaternary ammonium-based lipid (CTA14), and mono-head quaternary ammonium lipid (CPA14), and SE as helper lipid, were prepared into liposomes which could enhance the interactions between liposomes and pDNA. Most importantly, the liposomes with helper lipid SE showed higher transfection and lower cytotoxicity than those without SE in Hela and A549 cells. It was also found that the transfection efficiency increased with the increase of SE content. The selected liposome, CDO14/SE, was able to deliver siRNA against luciferase for silencing gene in lung tumors of mice, with little in vivo toxicity. The results convincingly demonstrated SEs could be highly desirable candidates for gene delivery systems.
authors.affiliation: SEAC-ME Key Laboratory of Biotechnology and Bioresources, Dalian Nationalities University, 116600 Dalian, China.
authors.forename: Yinan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: The Second Hospital of Dalian Medical University, 116023 Dalian, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhu
authors.affiliation: SEAC-ME Key Laboratory of Biotechnology and Bioresources, Dalian Nationalities University, 116600 Dalian, China.
authors.forename: Hengjun
authors.initials: H
authors.lastname: Zhou
authors.affiliation: College of Pharmacy, Dalian Medical University, 116044 Dalian, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Guo
authors.affiliation: SEAC-ME Key Laboratory of Biotechnology and Bioresources, Dalian Nationalities University, 116600 Dalian, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Tian
authors.affiliation: SEAC-ME Key Laboratory of Biotechnology and Bioresources, Dalian Nationalities University, 116600 Dalian, China.
authors.forename: Shaohui
authors.initials: S
authors.lastname: Cui
authors.affiliation: College of Pharmacy, Dalian Medical University, 116044 Dalian, China. Electronic address: zhenyuhong@gmail.com.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Zhen
authors.affiliation: SEAC-ME Key Laboratory of Biotechnology and Bioresources, Dalian Nationalities University, 116600 Dalian, China. Electronic address: zsb@dlnu.edu.cn.
authors.forename: Shubiao
authors.initials: S
authors.lastname: Zhang
authors.affiliation: College of Pharmacy, Shanghai Jiao Tong University, 200240 Shanghai, China.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Xu
mesh_terms: D000818:Animals; D002412:Cations; D002460:Cell Line; D045744:Cell Line, Tumor; D004337:Drug Carriers / Q000737:chemistry; D004952:Esters / Q000737:chemistry*; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D006367:HeLa Cells; D006801:Humans; D008081:Liposomes / Q000737:chemistry*; D008175:Lung Neoplasms / Q000628:therapy; D051379:Mice; D034741:RNA, Small Interfering / Q000235:genetics / Q000502:physiology; D013395:Sucrose / Q000737:chemistry*
chemical_list: D002412:Cations; D004337:Drug Carriers; D004952:Esters; D008081:Liposomes; D034741:RNA, Small Interfering; D013395:Sucrose
keywords: Cationic liposomes; Gene delivery; Helper lipids; Sucrose esters