title: Chair/bedside diagnosis of oral and respiratory tract infections, and identification of antibiotic resistances for personalised monitoring and treatment.
abstract: Global healthcare systems are struggling with the enormous burden associated with infectious diseases, as well as the incessant rise of antimicrobial resistance. In order to adequately address these issues, there is an urgent need for rapid and accurate infectious disease diagnostics. The H2020 project DIAGORAS aims at diagnosing oral and respiratory tract infections using a fully integrated, automated and user-friendly platform for physicians' offices, schools, elderly care units, community settings, etc. Oral diseases (periodontitis, dental caries) will be detected via multiplexed, quantitative analysis of salivary markers (bacterial DNA and host response proteins) for early prevention and personalised monitoring. Respiratory Tract Infections will be diagnosed by means of DNA/RNA differentiation so as to identify their bacterial or viral nature. Together with antibiotic resistance screening on the same platform, a more efficient treatment management is expected at the point-of-care. At the heart of DIAGORAS lies a centrifugal microfluidic platform (LabDisk and associated processing device) integrating all components and assays for a fully automated analysis. The project involves an interface with a clinical algorithm for the comprehensive presentation of results to end-users, thereby increasing the platform's clinical utility. DIAGORAS' performance will be validated at clinical settings and compared with gold standards.
authors.affiliation: Hahn-Schickard, Freiburg, Germany.
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Mitsakakis
authors.affiliation: Hahn-Schickard, Freiburg, Germany.
authors.forename: Fabian
authors.initials: F
authors.lastname: Stumpf
authors.affiliation: Hahn-Schickard, Freiburg, Germany.
authors.forename: Oliver
authors.initials: O
authors.lastname: Strohmeier
authors.affiliation: Hahn-Schickard, Freiburg, Germany.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Klein
authors.affiliation: Hahn-Schickard, Freiburg, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Mark
authors.affiliation: Hahn-Schickard, Freiburg, Germany.
authors.forename: Felix
authors.initials: F
authors.lastname: Von Stetten
authors.affiliation: Health & Environment Department, AIT Austrian Institute of Technology, Vienna Austria.
authors.forename: Johannes R
authors.initials: JR
authors.lastname: Peham
authors.affiliation: Health & Environment Department, AIT Austrian Institute of Technology, Vienna Austria.
authors.forename: Christopher
authors.initials: C
authors.lastname: Herz
authors.affiliation: Clinic of Preventive Dentistry, Periodontology and Cariology, Center of Dental Medicine, University of Zurich, Zurich, Switzerland.
authors.forename: Pune Nina
authors.initials: PN
authors.lastname: Tawakoli
authors.affiliation: Clinic of Preventive Dentistry, Periodontology and Cariology, Center of Dental Medicine, University of Zurich, Zurich, Switzerland.
authors.forename: Florian
authors.initials: F
authors.lastname: Wegehaupt
authors.affiliation: Clinic of Preventive Dentistry, Periodontology and Cariology, Center of Dental Medicine, University of Zurich, Zurich, Switzerland.
authors.forename: Thomas
authors.initials: T
authors.lastname: Attin
authors.affiliation: Division of Oral Microbiology and Immunology, Institute of Oral Biology, Center of Dental Medicine, University of Zurich, Zurich, Switzerland.
authors.forename: Nagihan
authors.initials: N
authors.lastname: Bostanci
authors.affiliation: Division of Oral Microbiology and Immunology, Institute of Oral Biology, Center of Dental Medicine, University of Zurich, Zurich, Switzerland.
authors.forename: Kai
authors.initials: K
authors.lastname: Bao
authors.affiliation: Division of Oral Microbiology and Immunology, Institute of Oral Biology, Center of Dental Medicine, University of Zurich, Zurich, Switzerland.
authors.forename: Georgios N
authors.initials: GN
authors.lastname: Belibasakis
authors.affiliation: Erasmus University Medical Centre, Rotterdam, The Netherlands.
authors.forename: John P
authors.initials: JP
authors.lastname: Hays
authors.affiliation: Erasmus University Medical Centre, Rotterdam, The Netherlands.
authors.forename: Gijs
authors.initials: G
authors.lastname: Elshout
authors.affiliation: Erasmus University Medical Centre, Rotterdam, The Netherlands.
authors.forename: Robin C
authors.initials: RC
authors.lastname: Huisman
authors.affiliation: Erasmus University Medical Centre, Rotterdam, The Netherlands.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Klein
authors.affiliation: Erasmus University Medical Centre, Rotterdam, The Netherlands.
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Stubbs
authors.affiliation: Askion GmbH, Gera, Germany.
authors.forename: Lutz
authors.initials: L
authors.lastname: Doms
authors.affiliation: Askion GmbH, Gera, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Wolf
authors.affiliation: MagnaMedics Diagnostics BV, Geleen, The Netherlands.
authors.forename: Viorel
authors.initials: V
authors.lastname: Rusu
authors.affiliation: MagnaMedics Diagnostics BV, Geleen, The Netherlands.
authors.forename: Sven
authors.initials: S
authors.lastname: Goethel
authors.affiliation: ClinicaGeno Ltd, London, United Kingdom.
authors.forename: Thomas
authors.initials: T
authors.lastname: Binsl
authors.affiliation: ClinicaGeno Ltd, London, United Kingdom.
authors.forename: Alex
authors.initials: A
authors.lastname: Michie
authors.affiliation: BioVendor - Laboratorni medicina a.s., Brno, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Jancovicova
authors.affiliation: BioVendor - Laboratorni medicina a.s., Brno, Czech Republic.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Kolar
authors.affiliation: BioVendor - Laboratorni medicina a.s., Brno, Czech Republic.
authors.forename: Michal
authors.initials: M
authors.lastname: Kostka
authors.affiliation: BioVendor - Laboratorni medicina a.s., Brno, Czech Republic.
authors.forename: Jiri
authors.initials: J
authors.lastname: Smutny
authors.affiliation: BioVendor - Laboratorni medicina a.s., Brno, Czech Republic.
authors.forename: Michal
authors.initials: M
authors.lastname: Karpisek
authors.affiliation: Sparks & Co, Paris, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Estephan
authors.affiliation: Sparks & Co, Paris, France.
authors.forename: Camille
authors.initials: C
authors.lastname: Cocaud
authors.affiliation: Hahn-Schickard, Freiburg, Germany.
authors.forename: Roland
authors.initials: R
authors.lastname: Zengerle
mesh_terms: D057205:Automation, Laboratory; D002498:Centrifugation / Q000379:methods; D004269:DNA, Bacterial / Q000032:analysis; D003731:Dental Caries / Q000175:diagnosis*; D024881:Drug Resistance, Bacterial; D006801:Humans; D046210:Microfluidic Analytical Techniques; D010518:Periodontitis / Q000175:diagnosis* / Q000382:microbiology; D057285:Precision Medicine / Q000379:methods; D012367:RNA, Viral / Q000032:analysis; D012141:Respiratory Tract Infections / Q000175:diagnosis* / Q000382:microbiology / Q000821:virology; D012463:Saliva / Q000276:immunology / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D012367:RNA, Viral