title: Hyperconcentrated Sweet Whey, a New Culture Medium That Enhances Propionibacterium freudenreichii Stress Tolerance.
abstract: Propionibacterium freudenreichii is used as a cheese-ripening starter and as a probiotic. Its reported physiological effects at the gut level, including modulation of bifidobacteria, colon epithelial cell proliferation and apoptosis, and intestinal inflammation, rely on active metabolism in situ Survival and activity are thus key factors determining its efficacy, creating stress adaptation and tolerance bottlenecks for probiotic applications. Growth media and growth conditions determine tolerance acquisition. We investigated the possibility of using sweet whey, a dairy by-product, to sustain P. freudenreichii growth. It was used at different concentrations (dry matter) as a culture medium. Using hyperconcentrated sweet whey led to enhanced multistress tolerance acquisition, overexpression of key stress proteins, and accumulation of intracellular storage molecules and compatible solutes, as well as enhanced survival upon spray drying. A simplified process from growth to spray drying of propionibacteria was developed using sweet whey as a 2-in-1 medium to both culture P. freudenreichii and protect it from heat and osmotic injury without harvesting and washing steps. As spray drying is far cheaper and more energy efficient than freeze-drying, this work opens new perspectives for the sustainable development of new starter and probiotic preparations with enhanced robustness.
  
  
  
  In this study, we demonstrate that sweet whey, a dairy industry by-product, not only allows the growth of probiotic dairy propionibacteria, but also triggers a multitolerance response through osmoadaptation and general stress response. We also show that propionibacteria accumulate compatible solutes under these culture conditions, which might account for the limited loss of viability after spray drying. This work opens new perspectives for more energy-efficient production of dairy starters and probiotics.
authors.affiliation: Suzhou Key Laboratory of Green Chemical Engineering, School of Chemical and Environmental Engineering, College of Chemistry, Chemical Engineering and Material Science, Soochow University, Jiangsu, China.|INRA, UMR1253 STLO, Science et Technologie du Lait et de l'Œuf, Rennes, France.|Agrocampus Ouest, UMR1253 STLO, Rennes, France.
authors.forename: Song
authors.initials: S
authors.lastname: Huang
authors.affiliation: INRA, UMR1253 STLO, Science et Technologie du Lait et de l'Œuf, Rennes, France.|Agrocampus Ouest, UMR1253 STLO, Rennes, France.
authors.forename: Houem
authors.initials: H
authors.lastname: Rabah
authors.affiliation: INRA, UMR1253 STLO, Science et Technologie du Lait et de l'Œuf, Rennes, France.|Agrocampus Ouest, UMR1253 STLO, Rennes, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Jardin
authors.affiliation: INRA, UMR1253 STLO, Science et Technologie du Lait et de l'Œuf, Rennes, France.|Agrocampus Ouest, UMR1253 STLO, Rennes, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Briard-Bion
authors.affiliation: INRA, UMR1253 STLO, Science et Technologie du Lait et de l'Œuf, Rennes, France.|Agrocampus Ouest, UMR1253 STLO, Rennes, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Parayre
authors.affiliation: INRA, UMR1253 STLO, Science et Technologie du Lait et de l'Œuf, Rennes, France.|Agrocampus Ouest, UMR1253 STLO, Rennes, France.
authors.forename: Marie-Bernadette
authors.initials: MB
authors.lastname: Maillard
authors.affiliation: INRA, UMR1253 STLO, Science et Technologie du Lait et de l'Œuf, Rennes, France.|Agrocampus Ouest, UMR1253 STLO, Rennes, France.
authors.forename: Yves
authors.initials: Y
authors.lastname: Le Loir
authors.affiliation: Suzhou Key Laboratory of Green Chemical Engineering, School of Chemical and Environmental Engineering, College of Chemistry, Chemical Engineering and Material Science, Soochow University, Jiangsu, China gwenael.jan@rennes.inra.fr xdcxmu@gmail.com.|Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, Fujian, China.
authors.forename: Xiao Dong
authors.initials: XD
authors.lastname: Chen
authors.affiliation: INRA, UMR1253 STLO, Science et Technologie du Lait et de l'Œuf, Rennes, France.|Agrocampus Ouest, UMR1253 STLO, Rennes, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Schuck
authors.affiliation: INRA, UMR1253 STLO, Science et Technologie du Lait et de l'Œuf, Rennes, France.|Agrocampus Ouest, UMR1253 STLO, Rennes, France.
authors.forename: Romain
authors.initials: R
authors.lastname: Jeantet
authors.affiliation: INRA, UMR1253 STLO, Science et Technologie du Lait et de l'Œuf, Rennes, France gwenael.jan@rennes.inra.fr xdcxmu@gmail.com.|Agrocampus Ouest, UMR1253 STLO, Rennes, France.
authors.forename: Gwénaël
authors.initials: G
authors.lastname: Jan
mesh_terms: D003470:Culture Media / Q000737:chemistry / Q000378:metabolism*; D000070002:Propionibacterium freudenreichii / Q000254:growth & development / Q000502:physiology*; D013312:Stress, Physiological; D000067796:Whey / Q000737:chemistry / Q000378:metabolism*
chemical_list: D003470:Culture Media