title: A rapid, highly sensitive and culture-free detection of pathogens from whole blood by removal of white blood cells using immuno-magnetic beads.
abstract: Using anti-human CD45 antibody coated beads, we show a 98% reduction of WBCs from spiked blood samples in 1h, thereby enriching it for pathogens. This enrichment allowed the detection of <10CFU of Escherichia coli in 1mL blood using quantitative PCR; something not observed in unenriched samples.
authors.affiliation: Siemens Healthcare Private Limited, 105, Prestige Alecto, Hosur Road, Bengaluru 560100, India.
authors.forename: Manjula Ramya
authors.initials: MR
authors.lastname: Vutukuru
authors.affiliation: Siemens Healthcare Private Limited, 105, Prestige Alecto, Hosur Road, Bengaluru 560100, India.
authors.forename: Divya Khandige
authors.initials: DK
authors.lastname: Sharma
authors.affiliation: Siemens Healthcare Private Limited, 105, Prestige Alecto, Hosur Road, Bengaluru 560100, India.
authors.forename: Ragavendar
authors.initials: R
authors.lastname: Ms
authors.affiliation: Siemens Healthcare Private Limited, 105, Prestige Alecto, Hosur Road, Bengaluru 560100, India. Electronic address: nivedita.mitra@siemens.com.
authors.forename: Nivedita
authors.initials: N
authors.lastname: Mitra
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D001769:Blood / Q000382:microbiology*; D004926:Escherichia coli / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000175:diagnosis; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D017493:Leukocyte Common Antigens / Q000276:immunology; D007962:Leukocytes; D057230:Limit of Detection; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D017493:Leukocyte Common Antigens; C516769:PTPRC protein, human
keywords: Analytical sensitivity; Blood stream infections; CD45; Culture-free detection; Healthcare acquired infections; Low pathogen load; Molecular diagnostics and culture-free detection of pathogens from whole blood; Pathogen enrichment; Quantitative PCR (qPCR); Sepsis