title: Diffuse optical microscopy for quantification of depth-dependent epithelial backscattering in the cervix.
abstract: A fiber optic imaging approach is presented using structured illumination for quantification of almost pure epithelial backscattering. We employ multiple spatially modulated projection patterns and camera-based reflectance capture to image depth-dependent epithelial scattering. The potential diagnostic value of our approach is investigated on cervical ex vivo tissue specimens. Our study indicates a strong backscattering increase in the upper part of the cervical epithelium caused by dysplastic microstructural changes. Quantization of relative depth-dependent backscattering is confirmed as a potentially useful diagnostic feature for detection of precancerous lesions in cervical squamous epithelium.
authors.affiliation: Institut für Lasertechnologien in der Medizin und Meßtechnik, Helmholtzstr. 12, D-89081 Ulm, GermanybBritish Columbia Cancer Research Centre, Cancer Imaging Department, 675 West 10th Avenue, Vancouver, BC V5Z 1L3, Canada.
authors.forename: Nico
authors.initials: N
authors.lastname: Bodenschatz
authors.affiliation: British Columbia Cancer Research Centre, Cancer Imaging Department, 675 West 10th Avenue, Vancouver, BC V5Z 1L3, Canada.
authors.forename: Sylvia
authors.initials: S
authors.lastname: Lam
authors.affiliation: British Columbia Cancer Research Centre, Cancer Imaging Department, 675 West 10th Avenue, Vancouver, BC V5Z 1L3, Canada.
authors.forename: Anita
authors.initials: A
authors.lastname: Carraro
authors.affiliation: British Columbia Cancer Research Centre, Cancer Imaging Department, 675 West 10th Avenue, Vancouver, BC V5Z 1L3, Canada.
authors.forename: Jagoda
authors.initials: J
authors.lastname: Korbelik
authors.affiliation: University of British Columbia, Division of Gynaecologic Oncology, 2775 Laurel Street, Vancouver, BC V5Z 1M9, Canada.
authors.forename: Dianne M
authors.initials: DM
authors.lastname: Miller
authors.affiliation: University of British Columbia, Division of Gynaecologic Oncology, 2775 Laurel Street, Vancouver, BC V5Z 1M9, Canada.
authors.forename: Jessica N
authors.initials: JN
authors.lastname: McAlpine
authors.affiliation: University of British Columbia, Division of Gynaecologic Oncology, 2775 Laurel Street, Vancouver, BC V5Z 1M9, Canada.
authors.forename: Marette
authors.initials: M
authors.lastname: Lee
authors.affiliation: Institut für Lasertechnologien in der Medizin und Meßtechnik, Helmholtzstr. 12, D-89081 Ulm, Germany.
authors.forename: Alwin
authors.initials: A
authors.lastname: Kienle
authors.affiliation: British Columbia Cancer Research Centre, Cancer Imaging Department, 675 West 10th Avenue, Vancouver, BC V5Z 1L3, Canada.
authors.forename: Calum
authors.initials: C
authors.lastname: MacAulay
mesh_terms: D002584:Cervix Uteri / Q000000981:diagnostic imaging*; D003952:Diagnostic Imaging / Q000379:methods*; D004848:Epithelium / Q000000981:diagnostic imaging*; D005260:Female; D006801:Humans; D008853:Microscopy; D061848:Optical Imaging; D002583:Uterine Cervical Neoplasms / Q000000981:diagnostic imaging*