title: Identification of key metabolic changes in renal interstitial fibrosis rats using metabonomics and pharmacology.
abstract: Renal fibrosis is one of the important pathways involved in end-stage renal failure. Investigating the metabolic changes in the progression of disease may enhance the understanding of its pathogenesis and therapeutic information. In this study, (1)H-nuclear magnetic resonance (NMR)-based metabonomics was firstly used to screen the metabolic changes in urine and kidney tissues of renal interstitial fibrotic rats induced by unilateral ureteral obstruction (UUO), at 7, 14, 21, and 28 days after operation, respectively. The results revealed that reduced levels of bioenergy synthesis and branched chain amino acids (BCAAs), as well as elevated levels of indoxyl sulfate (IS) are involved in metabolic alterations of renal fibrosis rats. Next, by pharmacological treatment we found that reduction of IS levels could prevent the renal fibrotic symptoms. Therefore, we suggested that urinary IS may be used as a potential biomarker for the diagnosis of renal fibrosis, and a therapeutic target for drugs. Novel attempt combining metabonomics and pharmacology was established that have ability to provide more systematic diagnostic and therapeutic information of diseases.
authors.affiliation: School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, China.
authors.forename: Liangcai
authors.initials: L
authors.lastname: Zhao
authors.affiliation: School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, China.
authors.forename: Minjian
authors.initials: M
authors.lastname: Dong
authors.affiliation: School &Hospital of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang, China.
authors.forename: Shixian
authors.initials: S
authors.lastname: Liao
authors.affiliation: School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Du
authors.affiliation: School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zheng
authors.affiliation: School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, China.|Lishui Central Hospital, the Fifth Affiliated Hospital, Wenzhou Medical University, Lishui, Zhejiang, China.
authors.forename: Minjiang
authors.initials: M
authors.lastname: Chen
authors.affiliation: Lishui Central Hospital, the Fifth Affiliated Hospital, Wenzhou Medical University, Lishui, Zhejiang, China.
authors.forename: Jiansong
authors.initials: J
authors.lastname: Ji
authors.affiliation: School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, China.
authors.forename: Hongchang
authors.initials: H
authors.lastname: Gao
mesh_terms: D000597:Amino Acids, Branched-Chain / Q000008:administration & dosage* / Q000494:pharmacology; D000818:Animals; D015415:Biomarkers / Q000652:urine; D004195:Disease Models, Animal; D007200:Indican / Q000652:urine*; D007668:Kidney / Q000737:chemistry / Q000187:drug effects; D007674:Kidney Diseases / Q000175:diagnosis / Q000188:drug therapy / Q000209:etiology / Q000378:metabolism*; D008297:Male; D055432:Metabolomics / Q000379:methods*; D066244:Proton Magnetic Resonance Spectroscopy; D051381:Rats; D017207:Rats, Sprague-Dawley; D014556:Urine / Q000737:chemistry
chemical_list: D000597:Amino Acids, Branched-Chain; D015415:Biomarkers; D007200:Indican