title: Protective Effects of Selenium, N-Acetylcysteine and Vitamin E Against Acute Ethanol Intoxication in Rats.
abstract: The aim of this study was to determine possible protective influences of selenium (Se), N-acetylcysteine (NAC), and vitamin E (Vit E) against acute ethanol (EtOH) intoxication. Thirty-six rats were divided into six groups: I (control), II (EtOH), III (EtOH + Se), IV (EtOH + Vit E), V (EtOH + NAC), and VI (EtOH + mix). Except group I, EtOH was given the other pretreated (groups III, IV, V, and VI) and untreated groups (group II). Compared with the EtOH group, serum aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, lactate dehydrogenase, creatine kinase, and creatine kinase-MB levels were significantly decreased in all pretreated groups, whereas slightly diminished amylase and lipase were observed. Compared with the control group, a remarkably lower total antioxidant status (TAS), but higher total oxidant status (TOS), and oxidative stress index (OSI) were seen in brain, liver, and kidney tissues. The values of these parameters were less affected from EtOH-exposed brain tissue of EtOH + NAC and liver of EtOH + mix groups. Both significant decrease of catalase activity and marked increases of adenosine deaminase and myeloperoxidase were determined only in liver tissue of the EtOH group. Activities of these enzymes were restored in almost all pretreated groups. Moreover, an increase of xanthine oxidase activity was prevented in brain tissue of pretreated groups. In histopathological examination of the liver, hydropic degeneration, sinusoidal dilatation, mononuclear cell infiltration, and marked congestion, which were seen in the EtOH group, were prevented in all pretreated groups. Relative protection against acute EtOH toxicity, in both single and combined pretreatments of Se, NAC, and Vit E supplementation, was probably through antioxidant and free radical-neutralizing effects of foregoing materials.
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Yuzuncu Yil University, Van, Turkey.
authors.forename: Halil
authors.initials: H
authors.lastname: Ozkol
authors.affiliation: Department of Pathology, Faculty of Medicine, Yuzuncu Yil University, Van, Turkey.
authors.forename: Gulay
authors.initials: G
authors.lastname: Bulut
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Yuzuncu Yil University, Van, Turkey.
authors.forename: Ragıp
authors.initials: R
authors.lastname: Balahoroglu
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Yuzuncu Yil University, Van, Turkey.
authors.forename: Yasin
authors.initials: Y
authors.lastname: Tuluce
authors.affiliation: Department of Dermatology, Faculty of Medicine, Yuzuncu Yil University, Van, Turkey. drhaticeuce@gmail.com.
authors.forename: Hatice Uce
authors.initials: HU
authors.lastname: Ozkol
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology*; D000208:Acute Disease; D000435:Alcoholic Intoxication / Q000378:metabolism* / Q000473:pathology / Q000517:prevention & control*; D000818:Animals; D008297:Male; D009928:Organ Specificity / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000494:pharmacology*; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D014810:Vitamin E; D012643:Selenium; D000111:Acetylcysteine
keywords: Acute ethanol toxicity; N-Acetylcysteine; Rat; Selenium; Vitamin E