title: Transciptomic study of mucosal immune, antioxidant and growth related genes and non-specific immune response of common carp (Cyprinus carpio) fed dietary Ferula (Ferula assafoetida).
abstract: A 8-weeks feeding trial was conducted to examine the effects of different levels (0, 0.5, 1 and 2%) of dietary Ferula (Ferula assafoetida) on expression of antioxidant enzymes (GSR, GPX and GSTA), immune (TNF-alpha, IL1B, IL- 8 and LYZ) and growth (GH, IGF1 and Ghrl) genes as well as cutaneous mucus and serum non-specific immune response in common carp. The results revealed Ferula significantly increased antioxidant gene expression (GSR and GSTA) in a dose dependent manner (P < 0.05). The expression of immune growth related genes were significantly higher in Ferula fed fish compared control group (P < 0.05). The effects of Ferula on expression of genes was more pronounced in higher doses. Feeding on Ferula supplemented diet remarkably increased skin mucus lysozyme activity (P < 0.05). However, evaluation of mucus total Ig and protease activity revealed no significant difference between control and treated groups (P > 0.05). Regarding non-specific humoral response, serum total Ig, lysozyme and ACH50 showed no remarkable variation between Ferula fed carps and control group (P > 0.05). These results indicated up-regulation of growth and health related genes in Ferula fed common carp. Further studies using pathogen or stress challenge is required to conclude that transcriptional modulation is beneficial in common carp.
authors.affiliation: Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. Electronic address: rsafari@gau.ac.ir.
authors.forename: Roghieh
authors.initials: R
authors.lastname: Safari
authors.affiliation: Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. Electronic address: hoseinifar@gau.ac.ir.
authors.forename: Seyed Hossein
authors.initials: SH
authors.lastname: Hoseinifar
authors.affiliation: Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
authors.forename: Shabnam
authors.initials: S
authors.lastname: Nejadmoghadam
authors.affiliation: Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Jafar
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002347:Carps / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D004032:Diet / Q000662:veterinary*; D019587:Dietary Supplements; D004305:Dose-Response Relationship, Drug; D005309:Ferula; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D000975:Antioxidants; D029941:Fish Proteins
keywords: Antioxidant defence; Ferula (Ferula assafoetida); Immune related gene expression; Mucosal immune response; Non-specific immunity