title: Melatonin delivery by nanocapsules during in vitro bovine oocyte maturation decreased the reactive oxygen species of oocytes and embryos.
abstract: In this work, a promising approach to increase the advantageous properties of melatonin through its encapsulation into lipid-core nanocapsules (LNC) was examined. Oocytes were treated during in vitro maturation with non-encapsulated melatonin (Mel), melatonin-loaded lipid-core nanocapsules (Mel-LNC), and unloaded LNC. Cytotoxicity, meiotic maturation rate, development to the blastocyst stage, reactive oxygen species (ROS) and glutathione levels, mean cell number and apoptotic cell/blastocyst, and mRNA quantification were evaluated. Both Mel and Mel-LNC enhanced in vitro embryo production, however, Mel-LNC proved to be more effective at decreasing ROS levels and the apoptotic cell number/blastocyst, increasing the cleavage and blastocyst rates, up-regulating the GPX1 and SOD2 genes, and down-regulating the CASP3 and BAX genes. Mel-LNC could penetrate into oocytes and remain inside the cells until they reach the blastocyst stage. In conclusion, when melatonin was encapsulated in LNC and applied during in vitro oocyte maturation, some quality aspects of the blastocysts were improved.
authors.affiliation: Programa de Pós-Graduação em Biotecnologia (PPGB), Grupo de Pesquisa em Oncologia Celular e Molecular, Biotecnologia/Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, 96010-900 RS, Brazil.
authors.forename: Mariana Härter
authors.initials: MH
authors.lastname: Remião
authors.affiliation: Programa de Pós-Graduação em Biotecnologia (PPGB), Grupo de Pesquisa em Oncologia Celular e Molecular, Biotecnologia/Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, 96010-900 RS, Brazil.
authors.forename: Caroline Gomes
authors.initials: CG
authors.lastname: Lucas
authors.affiliation: Programa de Pós-Graduação em Biotecnologia (PPGB), Grupo de Pesquisa em Oncologia Celular e Molecular, Biotecnologia/Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, 96010-900 RS, Brazil.
authors.forename: William Borges
authors.initials: WB
authors.lastname: Domingues
authors.affiliation: Programa de Pós-Graduação em Biotecnologia (PPGB), Grupo de Pesquisa em Oncologia Celular e Molecular, Biotecnologia/Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, 96010-900 RS, Brazil.
authors.forename: Tony
authors.initials: T
authors.lastname: Silveira
authors.affiliation: Programa de Pós-Graduação em Biotecnologia (PPGB), Grupo de Pesquisa em Oncologia Celular e Molecular, Biotecnologia/Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, 96010-900 RS, Brazil.
authors.forename: Nathaniele Nebel
authors.initials: NN
authors.lastname: Barther
authors.affiliation: Programa de Pós-Graduação em Biotecnologia (PPGB), Grupo de Pesquisa em Oncologia Celular e Molecular, Biotecnologia/Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, 96010-900 RS, Brazil.
authors.forename: Eliza Rossi
authors.initials: ER
authors.lastname: Komninou
authors.affiliation: In Vitro Brasil S/A, Mogi Mirim, 13800-970 SP, Brazil.
authors.forename: Andrea Cristina
authors.initials: AC
authors.lastname: Basso
authors.affiliation: Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Av. Ipiranga, 2752, Porto Alegre 90610-000, RS, Brazil.
authors.forename: Denise Soledade
authors.initials: DS
authors.lastname: Jornada
authors.affiliation: Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Av. Ipiranga, 2752, Porto Alegre 90610-000, RS, Brazil.
authors.forename: Ruy Carlos Ruver
authors.initials: RC
authors.lastname: Beck
authors.affiliation: Departamento de Química Orgânica, Instituto de Química, Universidade Federal do Rio Grande do Sul, Porto Alegre 91501-970, RS, Brazil.
authors.forename: Adriana Raffin
authors.initials: AR
authors.lastname: Pohlmann
authors.affiliation: Instituto de Ciências Biológicas, Universidade Federal do Rio Grande, Universidade Federal do Rio Grande, Rio Grande 96201-900, RS, Brazil.
authors.forename: Antonio Sérgio Varela
authors.initials: AS
authors.lastname: Junior
authors.affiliation: Programa de Pós-Graduação em Biotecnologia (PPGB), Grupo de Pesquisa em Oncologia Celular e Molecular, Biotecnologia/Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, 96010-900 RS, Brazil.
authors.forename: Fabiana Kömmling
authors.initials: FK
authors.lastname: Seixas
authors.affiliation: Programa de Pós-Graduação em Biotecnologia (PPGB), Grupo de Pesquisa em Oncologia Celular e Molecular, Biotecnologia/Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, 96010-900 RS, Brazil.
authors.forename: Vinicius Farias
authors.initials: VF
authors.lastname: Campos
authors.affiliation: Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Av. Ipiranga, 2752, Porto Alegre 90610-000, RS, Brazil.
authors.forename: Silvia Stanisçuaski
authors.initials: SS
authors.lastname: Guterres
authors.affiliation: Programa de Pós-Graduação em Biotecnologia (PPGB), Grupo de Pesquisa em Oncologia Celular e Molecular, Biotecnologia/Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, 96010-900 RS, Brazil. Electronic address: tiago.collares@pq.cnpq.br.
authors.forename: Tiago
authors.initials: T
authors.lastname: Collares
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage*; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000235:genetics; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects; D004622:Embryo, Mammalian / Q000187:drug effects; D047108:Embryonic Development / Q000187:drug effects; D005260:Female; D005307:Fertilization in Vitro; D018507:Gene Expression Regulation, Developmental; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000235:genetics; D008550:Melatonin / Q000008:administration & dosage*; D053769:Nanocapsules / Q000008:administration & dosage*; D009865:Oocytes / Q000187:drug effects* / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000235:genetics; D051028:bcl-2-Associated X Protein / Q000235:genetics; D000094765:Glutathione Peroxidase GPX1; D000099156:Superoxide Dismutase 2
chemical_list: D000975:Antioxidants; D053148:Caspase 3; D005978:Glutathione; D005979:Glutathione Peroxidase; D008550:Melatonin; D053769:Nanocapsules; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase; D051028:bcl-2-Associated X Protein; D000094765:Glutathione Peroxidase GPX1; D000099156:Superoxide Dismutase 2
keywords: Antioxidant; Bovine oocyte; In vitro maturation; Lipid-core; Melatonin; Nanocapsules; Nanotechnology