title: Subtle genotypic changes can be observed soon after diagnosis in Mycobacterium tuberculosis infection.
abstract: Clonal variants of Mycobacterium tuberculosis (MTB) coexist in specific patients, although the dynamics of their emergence is unknown. We used MIRU-VNTR to detect microevolution leading to variants of MTB in 3 out of 19 patients (15%) soon after diagnosis (61-85days). Most harbored SNPs and for some of them a potential functional role was suggested. Microevolution in tuberculosis seems to occur sooner and more often than expected and could affect tracking of transmission.
authors.affiliation: Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain; CIBER Enfermedades respiratorias, CIBERES, Spain. Electronic address: lperezg00@gmail.com.
authors.forename: Laura
authors.initials: L
authors.lastname: Pérez-Lago
authors.affiliation: Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain.
authors.forename: Ana Isabel
authors.initials: AI
authors.lastname: Rodríguez Borlado
authors.affiliation: Unidad Mixta Genómica y Salud, Centro Superior de Investigación en Salud Pública (FISABIO), Universitat de València, Valencia, Spain; CIBER en Epidemiología y Salud Pública CIBERESP, Spain.
authors.forename: Iñaki
authors.initials: I
authors.lastname: Comas
authors.affiliation: Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain; CIBER Enfermedades respiratorias, CIBERES, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Herranz
authors.affiliation: Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain; CIBER Enfermedades respiratorias, CIBERES, Spain.
authors.forename: María Jesús
authors.initials: MJ
authors.lastname: Ruiz-Serrano
authors.affiliation: Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain; CIBER Enfermedades respiratorias, CIBERES, Spain; Departamento de Medicina, Facultad de Medicina, Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: Emilio
authors.initials: E
authors.lastname: Bouza
authors.affiliation: Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain; CIBER Enfermedades respiratorias, CIBERES, Spain; CEI Campus Moncloa, UCM-UPM, Madrid, Spain. Electronic address: dgviedma2@gmail.com.
authors.forename: Darío
authors.initials: D
authors.lastname: García-de-Viedma
mesh_terms: D000220:Adaptation, Biological; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D019143:Evolution, Molecular; D005260:Female; D014644:Genetic Variation; D060005:Genotyping Techniques; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000302:isolation & purification*; D020641:Polymorphism, Single Nucleotide; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology*
keywords: Clonal complexity; MIRU-VNTR; Microevolution; Tuberculosis