title: [Ribonuclease binase induces death in T-cell acute lymphoblastic leukemia cells by apoptosis].
abstract: Bacterial ribonuclease binase is a potential anticancer agent. In the present study, we have determined the toxic effect of binase towards cell lines of T-cell acute lymphoblastic leukemia Jurkat and CEMss. We have shown that binase induces apoptosis in these cells. At the same time, binase does not cause toxic effects in leukocytes of healthy donors, which suggests that binase activity towards leukemic cells is selective. We have found that the treatment of cancer cells with binase leads to a reduction in reactive oxygen species and transcription factor NFκB levels, and demonstrated that these effects are a common feature of the action of RNases on cancer cells.
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
authors.forename: K M
authors.initials: KM
authors.lastname: Burnysheva
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
authors.forename: I Yu
authors.initials: IY
authors.lastname: Petrushanko
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
authors.forename: P V
authors.initials: PV
authors.lastname: Spirin
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
authors.forename: V S
authors.initials: VS
authors.lastname: Prassolov
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Makarov
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.|mitkevich@gmail.com.
authors.forename: V A
authors.initials: VA
authors.lastname: Mitkevich
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001407:Bacillus / Q000201:enzymology; D049109:Cell Proliferation / Q000187:drug effects*; D004722:Endoribonucleases / Q000008:administration & dosage*; D006801:Humans; D019169:Jurkat Cells; D016328:NF-kappa B / Q000378:metabolism; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000188:drug therapy* / Q000473:pathology; D017382:Reactive Oxygen Species / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism
chemical_list: D016328:NF-kappa B; D017382:Reactive Oxygen Species; D004722:Endoribonucleases; C022543:ribonuclease T(2)
keywords: Ribonuclease; apoptosis; cytotoxicity; mitochondrial membrane potential; reactive oxygen species; transcription factor NFκB