title: Insertion and Deletion Mismatches Distant from the Target Position Improve Gene Correction with a Tailed Duplex.
abstract: A 5'-tailed duplex (TD) DNA corrects a base-substitution mutation. In this study, the effects of insertion and deletion (indel) mismatches distant from the target position on the gene correction were examined. Three target plasmid DNAs with and without indel mismatches ∼330 bases distant from the correction target position were prepared, and introduced into HeLa cells together with the TD. The indel mismatches improved the gene correction efficiency and specificity without sequence conversions at the indel mismatch site. These results suggested that the gene correction efficiency and specificity are increased when an appropriate second mismatch is introduced into the TD fragment.
authors.affiliation: a Graduate School of Science and Engineering, Ehime University , Matsuyama , Japan.|b Graduate School of Biomedical and Health Sciences, Hiroshima University , Minami-ku, Hiroshima , Japan.|c Faculty of Pharmaceutical Sciences, Hokkaido University , Sapporo , Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kamiya
authors.affiliation: a Graduate School of Science and Engineering, Ehime University , Matsuyama , Japan.|b Graduate School of Biomedical and Health Sciences, Hiroshima University , Minami-ku, Hiroshima , Japan.
authors.forename: Natsuki
authors.initials: N
authors.lastname: Nishigaki
authors.affiliation: a Graduate School of Science and Engineering, Ehime University , Matsuyama , Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ikeda
authors.affiliation: a Graduate School of Science and Engineering, Ehime University , Matsuyama , Japan.
authors.forename: Seiya
authors.initials: S
authors.lastname: Yukawa
authors.affiliation: c Faculty of Pharmaceutical Sciences, Hokkaido University , Sapporo , Japan.
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Morita
authors.affiliation: d Graduate School of Medical Sciences, Kyushu University , Higashi-ku, Fukuoka , Japan.
authors.forename: Yoshimichi
authors.initials: Y
authors.lastname: Nakatsu
authors.affiliation: d Graduate School of Medical Sciences, Kyushu University , Higashi-ku, Fukuoka , Japan.
authors.forename: Teruhisa
authors.initials: T
authors.lastname: Tsuzuki
authors.affiliation: c Faculty of Pharmaceutical Sciences, Hokkaido University , Sapporo , Japan.
authors.forename: Hideyoshi
authors.initials: H
authors.lastname: Harashima
mesh_terms: D020137:Base Pair Mismatch; D001483:Base Sequence; D053843:DNA Mismatch Repair; D006367:HeLa Cells; D006801:Humans; D054643:INDEL Mutation; D010957:Plasmids / Q000235:genetics; D017422:Sequence Analysis, DNA; D052416:Targeted Gene Repair / Q000379:methods*
keywords: Indel mismatch; gene correction; nucleotide sequence conversion; rpsL gene; tailed duplex