title: Aurora-A recruitment and centrosomal maturation are regulated by a Golgi-activated pool of Src during G2.
abstract: The Golgi apparatus is composed of stacks of cisternae laterally connected by tubules to form a ribbon-like structure. At the onset of mitosis, the Golgi ribbon is broken down into discrete stacks, which then undergo further fragmentation. This ribbon cleavage is required for G2/M transition, which thus indicates that a 'Golgi mitotic checkpoint' couples Golgi inheritance with cell cycle transition. We previously showed that the Golgi-checkpoint regulates the centrosomal recruitment of the mitotic kinase Aurora-A; however, how the Golgi unlinking regulates this recruitment was unknown. Here we show that, in G2, Aurora-A recruitment is promoted by activated Src at the Golgi. Our data provide evidence that Src and Aurora-A interact upon Golgi ribbon fragmentation; Src phosphorylates Aurora-A at tyrosine 148 and this specific phosphorylation is required for Aurora-A localization at the centrosomes. This process, pivotal for centrosome maturation, is a fundamental prerequisite for proper spindle formation and chromosome segregation.
authors.affiliation: Institute of Protein Biochemistry (IBP), National Research Council (CNR), Via P. Castellino 111, 80131 Naples, Italy.
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Barretta
authors.affiliation: Institute of Protein Biochemistry (IBP), National Research Council (CNR), Via P. Castellino 111, 80131 Naples, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Spano
authors.affiliation: Proteomics and Mass Spectrometry Laboratory, Institute for the Animal Production System in the Mediterranean Environment, ISPAAM, National Research Council (CNR), Via Argine 1085, 80147 Naples, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: D'Ambrosio
authors.affiliation: Institute of Protein Biochemistry (IBP), National Research Council (CNR), Via P. Castellino 111, 80131 Naples, Italy.
authors.forename: Romina Ines
authors.initials: RI
authors.lastname: Cervigni
authors.affiliation: Proteomics and Mass Spectrometry Laboratory, Institute for the Animal Production System in the Mediterranean Environment, ISPAAM, National Research Council (CNR), Via Argine 1085, 80147 Naples, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Scaloni
authors.affiliation: Institute of Protein Biochemistry (IBP), National Research Council (CNR), Via P. Castellino 111, 80131 Naples, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Corda
authors.affiliation: Institute of Protein Biochemistry (IBP), National Research Council (CNR), Via P. Castellino 111, 80131 Naples, Italy.
authors.forename: Antonino
authors.initials: A
authors.lastname: Colanzi
mesh_terms: D000818:Animals; D064096:Aurora Kinase A / Q000235:genetics / Q000502:physiology*; D000081247:CSK Tyrosine-Protein Kinase; D018385:Centrosome / Q000502:physiology*; D020090:Chromosome Segregation / Q000502:physiology; D016195:G2 Phase / Q000502:physiology*; D006056:Golgi Apparatus / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D007211:Indoles / Q000494:pharmacology; D010766:Phosphorylation; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D051381:Rats; D016196:S Phase / Q000187:drug effects; D013449:Sulfonamides / Q000494:pharmacology; D013936:Thymidine / Q000494:pharmacology; D014443:Tyrosine / Q000378:metabolism; D019061:src-Family Kinases / Q000037:antagonists & inhibitors / Q000502:physiology*
chemical_list: D007211:Indoles; D034741:RNA, Small Interfering; C416927:SU 6656; D013449:Sulfonamides; D014443:Tyrosine; D000081247:CSK Tyrosine-Protein Kinase; D019061:src-Family Kinases; C000631346:CSK protein, human; C576917:AURKA protein, human; D064096:Aurora Kinase A; D013936:Thymidine