title: Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate.
abstract: The present study investigated preparation of bovine and porcine erythrocyte membranes from slaughterhouse blood as bio-derived materials for delivery of dexamethasone-sodium phosphate (DexP). The obtained biomembranes, i.e., ghosts were characterized in vitro in terms of morphological properties, loading parameters, and release behavior. For the last two, an UHPLC/-HESI-MS/MS based analytical procedure for absolute drug identification and quantification was developed. The results revealed that loading of DexP into both type of ghosts was directly proportional to the increase of drug concentration in the incubation medium, while incubation at 37°C had statistically significant effect on loaded amount of DexP (P < 0.05). The encapsulation efficiency was about fivefold higher in porcine compared to bovine ghosts. Insight into ghosts' surface morphology by field emission-scanning electron microscopy and atomic force microscopy confirmed that besides inevitable effects of osmosis, DexP inclusion itself had no observable additional effect on the morphology of the ghosts carriers. DexP release profiles were dependent on erythrocyte ghost type and amount of residual hemoglobin. However, sustained DexP release was achieved and shown over 3 days from porcine ghosts and 5 days from bovine erythrocyte ghosts. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1046-1055, 2016.
authors.affiliation: Dept. of Chemical Engineering, Faculty of Technology and Metallurgy, University of Belgrade, Belgrade, Serbia.
authors.forename: Ivana T
authors.initials: IT
authors.lastname: Drvenica
authors.affiliation: Dept. of Chemical Engineering, Faculty of Technology and Metallurgy, University of Belgrade, Belgrade, Serbia.
authors.forename: Katarina M
authors.initials: KM
authors.lastname: Bukara
authors.affiliation: Inst. for Medical Research, University of Belgrade, Belgrade, Serbia.
authors.forename: Vesna Lj
authors.initials: VLj
authors.lastname: Ilić
authors.affiliation: Inst. for Biological Research "Siniša Stanković,", University of Belgrade, Belgrade, Serbia.
authors.forename: Danijela M
authors.initials: DM
authors.lastname: Mišić
authors.affiliation: Inst. of Physics, University of Belgrade, Belgrade, Serbia.
authors.forename: Borislav Z
authors.initials: BZ
authors.lastname: Vasić
authors.affiliation: Inst. of Physics, University of Belgrade, Belgrade, Serbia.
authors.forename: Radoš B
authors.initials: RB
authors.lastname: Gajić
authors.affiliation: Dept. of Chemical Engineering, Faculty of Technology and Metallurgy, University of Belgrade, Belgrade, Serbia.
authors.forename: Verica B
authors.initials: VB
authors.lastname: Đorđević
authors.affiliation: Dept. of Chemical Engineering, Faculty of Technology and Metallurgy, University of Belgrade, Belgrade, Serbia.
authors.forename: Đorđe N
authors.initials: ĐN
authors.lastname: Veljović
authors.affiliation: Inst. of Physics, University of Belgrade, Belgrade, Serbia.
authors.forename: Aleksandar
authors.initials: A
authors.lastname: Belić
authors.affiliation: Dept. of Chemical Engineering, Faculty of Technology and Metallurgy, University of Belgrade, Belgrade, Serbia.
authors.forename: Branko M
authors.initials: BM
authors.lastname: Bugarski
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003907:Dexamethasone / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000378:metabolism*; D004912:Erythrocytes / Q000737:chemistry / Q000378:metabolism*; D013552:Swine; D053719:Tandem Mass Spectrometry; D013997:Time Factors
chemical_list: C004180:dexamethasone 21-phosphate; D003907:Dexamethasone
keywords: biomembranes; dexamethasone sodium phosphate; drug delivery systems; erythrocyte ghosts; slaughterhouse blood