title: Tissue Inhibitor of Matrix Metalloproteinases-1 Knockdown Suppresses the Proliferation of Human Adipose-Derived Stem Cells.
abstract: Tissue inhibitor of metalloproteinases-1 (TIMP-1) is a multifunctional matrix metalloproteinase, and it is involved in the regulation of cell proliferation and apoptosis in various cell types. However, little is known about the effect of TIMP-1 expression on the proliferation of adipose-derived stem cells (ADSCs). Therefore, TIMP-1 expression in the ADSCs was firstly detected by western blotting, and TIMP-1 gene was knocked down by lentivirus-mediated shRNA. Cell proliferation was then evaluated by MTT assay and Ki67 staining, respectively. Cell cycle progression was determined by flow cytometry. The changes of p51, p21, cyclin E, cyclin-dependent kinase 2 (CDK2), and P-CDK2 caused by TIMP-1 knockdown were detected by western blotting. The results indicated that ADSCs highly expressed TIMP-1 protein, and the knockdown of TIMP-1 inhibited cell proliferation and arrested cell cycle progression at G1 phase in the ADSCs possibly through the upregulation of p53, p21, and P-CDK2 protein levels and concurrent downregulation of cyclin E and CDK2 protein levels. These findings suggest that TIMP-1 works as a positive regulator of cell proliferation in ADSCs.
authors.affiliation: Institute of Plastic Surgery, Stem Cell Research and Clinical Translation Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong 524001, China.
authors.forename: Peihua
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Institute of Plastic Surgery, Stem Cell Research and Clinical Translation Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong 524001, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Li
authors.affiliation: Institute of Plastic Surgery, Stem Cell Research and Clinical Translation Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong 524001, China.
authors.forename: Yawei
authors.initials: Y
authors.lastname: Qi
authors.affiliation: Institute of Biochemistry and Molecular Biology, Guangdong Medical University, Zhanjiang, Guangdong 524023, China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Tang
authors.affiliation: Institute of Plastic Surgery, Stem Cell Research and Clinical Translation Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong 524001, China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Duan
authors.affiliation: Institute of Plastic Surgery, Stem Cell Research and Clinical Translation Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong 524001, China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: Institute of Plastic Surgery, Stem Cell Research and Clinical Translation Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong 524001, China.
authors.forename: Zeyong
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Institute of Plastic Surgery, Stem Cell Research and Clinical Translation Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong 524001, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liang
authors.affiliation: Institute of Plastic Surgery, Stem Cell Research and Clinical Translation Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong 524001, China.
authors.forename: Jiangfeng
authors.initials: J
authors.lastname: Li
authors.affiliation: Institute of Plastic Surgery, Stem Cell Research and Clinical Translation Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong 524001, China.
authors.forename: Xian
authors.initials: X
authors.lastname: Wang
authors.affiliation: Institute of Plastic Surgery, Stem Cell Research and Clinical Translation Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong 524001, China.
authors.forename: Guofang
authors.initials: G
authors.lastname: Zeng
authors.affiliation: Department of Plastic Surgery, The First Affiliated Hospital of Jinan University, Key Laboratory for Regenerative Medicine, Ministry of Education, Guangzhou, Guangdong 510630, China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Liu