title: Sec15 links bud site selection to polarised cell growth and exocytosis in Candida albicans.
abstract: The exocyst plays a crucial role in the targeting of secretory vesicles to the plasma membrane during exocytosis. It has been shown to be involved in diverse cellular processes including yeast budding. However, the mechanism of the exocyst regulating yeast budding has not been fully elucidated. Here we report a novel interaction between the exocyst component Sec15 and the Ras-family GTPase Rsr1, a master regulator of bud-site-selection system, in the fungus Candida albicans. We present several lines of evidence indicating physical and genetic interaction of Sec15 with Rsr1. In vitro binding assays and co-immunoprecipitation studies showed that Sec15 associated physically with Rsr1. Deletion of RSR1 completely abolished the polarised localisation of Sec15 as well as all the other exocyst components in both yeast and hyphal cells, suggesting a functional interaction between Sec15 and Rsr1. We also show that C. albicans Sec15 interacts directly with the polarity determinant Bem1 and the type V myosin, Myo2. Disruption of the interaction by shutting off SEC15 results in mislocaliztion of Bem1-GFP. These findings highlight the important role of Sec15 in polarised cell growth by providing a direct functional link between bud-site-selection and exocytosis.
authors.affiliation: Institute of Health Sciences, Anhui University, Hefei 230601, China.
authors.forename: Pan Pan
authors.initials: PP
authors.lastname: Guo
authors.affiliation: Institute of Molecular and Cell Biology, A*STAR, 61 Biopolis Drive, 138673 Singapore.
authors.forename: Jie Ying Au
authors.initials: JY
authors.lastname: Yong
authors.affiliation: Institute of Molecular and Cell Biology, A*STAR, 61 Biopolis Drive, 138673 Singapore.
authors.forename: Yan Ming
authors.initials: YM
authors.lastname: Wang
authors.affiliation: Institute of Health Sciences, Anhui University, Hefei 230601, China.
authors.forename: Chang Run
authors.initials: CR
authors.lastname: Li
mesh_terms: D002176:Candida albicans / Q000254:growth & development* / Q000378:metabolism; D002453:Cell Cycle; D005089:Exocytosis; D025301:Hyphae / Q000254:growth & development / Q000378:metabolism; D020691:rab GTP-Binding Proteins / Q000378:metabolism*; D018631:ras Proteins / Q000378:metabolism*
chemical_list: D020691:rab GTP-Binding Proteins; D018631:ras Proteins