title: Near-Infrared Fluorescence Endoscopy to Detect Dysplastic Lesions in the Mouse Colon.
abstract: Near-infrared fluorescence (NIRF) endoscopy has a great potential for efficient early detection of dysplastic lesions in the colon. For preclinical studies, we developed a small animal NIRF endoscope and successfully used this device to identify dysplastic lesions in a murine model of chronic colitis. In this chapter, we present a step-by-step protocol for using NIRF endoscopy to examine the location, the size, and the borders of the dysplastic lesions developed in murine colitis. Our studies suggest that NIRF endoscopy is a specific and sensitive technique that provides a unique opportunity to analyze early stages of tumorigenesis in animal models of colon cancer and to perform surveillance colonoscopy in patients with colitis-associated colon cancer.
authors.affiliation: Department of Surgery, Northwestern University Feinberg School of Medicine, 303 East Superior Street, Lurie 3-104, Chicago, IL, 60611, USA. gounaris@northwestern.edu.|Robert H. Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine, 303 East Superior Street, Lurie 3-104, Chicago, IL, 60611, USA. gounaris@northwestern.edu.
authors.forename: Elias
authors.initials: E
authors.lastname: Gounaris
authors.affiliation: Molecular Diagnostic Technology Group, Advanced Core Technology Department, Research and Development Division, Olympus Tokyo, Tokyo, Japan.
authors.forename: Yasushige
authors.initials: Y
authors.lastname: Ishihara
authors.affiliation: Department of Surgery, Northwestern University Feinberg School of Medicine, 303 East Superior Street, Lurie 3-104, Chicago, IL, 60611, USA.
authors.forename: Manisha
authors.initials: M
authors.lastname: Shrivastrav
authors.affiliation: Department of Surgery, Northwestern University Feinberg School of Medicine, 303 East Superior Street, Lurie 3-104, Chicago, IL, 60611, USA.|Robert H. Lurie Comprehensive Cancer Center, Northwestern University Feinberg School of Medicine, 303 East Superior Street, Lurie 3-104, Chicago, IL, 60611, USA.|Jesse Brown Veterans Affairs Medical Center, Chicago, IL, USA.
authors.forename: David
authors.initials: D
authors.lastname: Bentrem
authors.affiliation: Division of Gastroenterology, Department of Medicine, University of Kentucky, Lexington, KY, USA.
authors.forename: Terrence A
authors.initials: TA
authors.lastname: Barrett
mesh_terms: D000818:Animals; D003093:Colitis, Ulcerative / Q000150:complications* / Q000000981:diagnostic imaging; D003106:Colon / Q000473:pathology; D004195:Disease Models, Animal; D055088:Early Detection of Cancer; D020776:Endoscopes, Gastrointestinal; D051379:Mice; D011230:Precancerous Conditions / Q000000981:diagnostic imaging*; D019265:Spectroscopy, Near-Infrared / Q000295:instrumentation*
keywords: Cathepsin activity; Colon cancer; Endoscopy; Mouse colon dysplasia; Mucosal inflammation; Near-infrared probes