title: Effect of propolis in gastric disorders: inhibition studies on the growth of Helicobacter pylori and production of its urease.
abstract: There is considerable interest in alternative approaches to inhibit Helicobacter pylori (H. pylori) and thus treat many stomach diseases. Propolis is a pharmaceutical mixture containing many natural bioactive substances. The aim of this study was to use propolis samples to treat H. pylori. The anti-H. pylori and anti-urease activities of 15 different ethanolic propolis extracts (EPEs) were tested. The total phenolic contents and total flavonoid contents of the EPE were also measured. The agar-well diffusion assay was carried out on H. pylori strain J99 and the inhibition zones were measured and compared with standards. All propolis extracts showed high inhibition of H. pylori J99, with inhibition diameters ranging from 31.0 to 47.0 mm. Helicobacter pylori urease inhibitory activity was measured using the phenol-hypochlorite assay; all EPEs showed significant inhibition against the enzyme, with inhibition concentrations (IC50; mg/mL) ranging from 0.260 to 1.525 mg/mL. The degree of inhibition was related to the phenolic content of the EPE. In conclusion, propolis extract was found to be a good inhibitor that can be used in H. pylori treatment to improve human health.
authors.affiliation: a Department of Chemistry and.
authors.forename: Nimet
authors.initials: N
authors.lastname: Baltas
authors.affiliation: b Department of Biology , Faculty of Arts and Science, Recep Tayyip Erdogan University , Rize , Turkey , and.
authors.forename: Sengul Alpay
authors.initials: SA
authors.lastname: Karaoglu
authors.affiliation: b Department of Biology , Faculty of Arts and Science, Recep Tayyip Erdogan University , Rize , Turkey , and.
authors.forename: Cemre
authors.initials: C
authors.lastname: Tarakci
authors.affiliation: c Department of Chemistry , Faculty of Science, Karadeniz Technical University , Trabzon , Turkey.
authors.forename: Sevgi
authors.initials: S
authors.lastname: Kolayli
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D016480:Helicobacter pylori / Q000187:drug effects* / Q000201:enzymology* / Q000254:growth & development; D006801:Humans; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D011429:Propolis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013272:Stomach Diseases / Q000188:drug therapy* / Q000382:microbiology*; D013329:Structure-Activity Relationship; D014510:Urease / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D004791:Enzyme Inhibitors; D011429:Propolis; D014510:Urease
keywords: Helicobacter pylori; inhibition; propolis; urease