title: Effects of Parental Dietary Exposure to GM Rice TT51 on the Male Reproductive System of Rat Offspring.
abstract: OBJECTIVE
  To evaluate the health effects of parental dietary exposure to GM rice TT51 on the male reproductive system of rat off spring.
  
  
  METHODS
  Rice-based diets, containing 60% ordinary grocery rice, MingHui63, or TT51 by weight, were given to parental rats (15 males/30 females each group) for 70 days prior mating and throughout pregnancy and lactation. After weaning, eight male offspring rats were randomly selected at each group and fed with diets correspondent to their parents' for 70 days. The effects of exposure to TT51 on male reproductive system of offspring rats were assessed through sperm parameters, testicular function enzyme activities, serum hormones (FSH, LH, and testosterone levels), testis histopathological examination, and the relative expression levels of selected genes along the hypothalamic-pituitary- testicular (HPT) axis.
  
  
  RESULTS
  No significant differences were observed in body weight, food intake, organ/body weights, serum hormone, sperm parameters, testis function enzyme ACP, LDH, and SDH activities, testis histopathological changes, and relative mRNA expression levels of GnRH-R, FSH-R, LH-R, and AR along the HPT axis.
  
  
  CONCLUSIONS
  The results of this study demonstrate that parental dietary exposure to TT51 reveals no significant differences on the reproductive system of male offspring rats compared with MingHui63 and control.
authors.affiliation: Department of Nutrition and Food Hygiene, College of Public Health, Xinxiang Medical University, Xinxiang 453003, Henan, China; Henan Collaborative Innovation Center of Molecular Diagnosis and Laboratory Medicine, Xinxiang Medical University, Xinxiang 453003, Henan, China; China National Center for Food Safety Risk Assessment, Beijing 100021, China.
authors.forename: Er Hui
authors.initials: EH
authors.lastname: Wang
authors.affiliation: China National Center for Food Safety Risk Assessment, Beijing 100021, China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Yu
authors.affiliation: China National Center for Food Safety Risk Assessment, Beijing 100021, China.
authors.forename: Xu Dong
authors.initials: XD
authors.lastname: Jia
authors.affiliation: China National Center for Food Safety Risk Assessment, Beijing 100021, China.
authors.forename: Wen Zhong
authors.initials: WZ
authors.lastname: Zhang
authors.affiliation: China National Center for Food Safety Risk Assessment, Beijing 100021, China.
authors.forename: Hai Bin
authors.initials: HB
authors.lastname: Xu
mesh_terms: D000818:Animals; D004032:Diet / Q000009:adverse effects; D005260:Female; D005837:Genitalia, Male / Q000502:physiology; D008297:Male; D012275:Oryza / Q000737:chemistry*; D030821:Plants, Genetically Modified / Q000009:adverse effects / Q000737:chemistry*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
keywords: Genetically modified rice; Hypothalamic-pituitary-testicular axis; Reproductive toxicity; Sperm parameter