title: Novel approaches to map small molecule-target interactions.
abstract: The quest for small molecule perturbators of protein function or a given cellular process lies at the heart of chemical biology and pharmaceutical research. Bioactive compounds need to be extensively characterized in the context of the modulated protein(s) or process(es) in living systems to unravel and confirm their mode of action. A crucial step in this workflow is the identification of the molecular targets for these small molecules, for which a generic methodology is lacking. Herein we summarize recently developed approaches for target identification spurred by advances in omics techniques and chemo- and bioinformatics analysis.
authors.affiliation: Max Planck Institute of Molecular Physiology, Otto-Hahn-Strasse 11, Dortmund, Germany. Electronic address: shobhna.kapoor@mpi-dortmund.mpg.de.
authors.forename: Shobhna
authors.initials: S
authors.lastname: Kapoor
authors.affiliation: Max Planck Institute of Molecular Physiology, Otto-Hahn-Strasse 11, Dortmund, Germany; Technical University Dortmund, Department of Chemistry and Chemical Biology, Otto-Hahn-Strasse 6, Dortmund, Germany.
authors.forename: Herbert
authors.initials: H
authors.lastname: Waldmann
authors.affiliation: Max Planck Institute of Molecular Physiology, Otto-Hahn-Strasse 11, Dortmund, Germany. Electronic address: slava.ziegler@mpi-dortmund.mpg.de.
authors.forename: Slava
authors.initials: S
authors.lastname: Ziegler
mesh_terms: D055808:Drug Discovery / Q000379:methods*; D058990:Molecular Targeted Therapy / Q000379:methods*; D054852:Small Molecule Libraries / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D054852:Small Molecule Libraries
keywords: Bioactive small molecules; CETSA, transcriptome sequencing; CRISPR/Cas9; Chem-seq; Profiling; Target identification; Target prediction