title: Laser Induced Forward Transfer: a study on the enhancement of sensitivity for multianalyte sensing.
abstract: In this article novel approaches for the improvement of the recorded signal coupled with the feasibility of multiple analyte detection, irrespective of the biosensor platform are being presented. The techniques that have been developed address commonly encountered issues that have traditionally hindered the commercialization of biosensors, such as cost, reproducibility and sensitivity and most importantly multianalyte detection. The fluorescence-based detection of copper is being described as an example of the use of Laser Induced Forward Transfer technique (LIFT) for the immobilization of biomolecules with high spatial resolution, in addition to a technique that involves the displacement of a short complementary strand to the immobilized probe molecule for the quantification of analyte binding and the enhancement of the recorded signal.
authors.affiliation: Biomedical Research Foundation of the Academy of Athens, Soranou Ephessiou 4, 11527 Athens, Greece.
authors.forename: George
authors.initials: G
authors.lastname: Tsekenis
authors.affiliation: National Technical University of Athens, Physics Department, Iroon Polytechneiou 9, 15780 Zografou, Athens, Greece.
authors.forename: Ioannis
authors.initials: I
authors.lastname: Theodorakos
authors.affiliation: National Technical University of Athens, Physics Department, Iroon Polytechneiou 9, 15780 Zografou, Athens, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Massaouti
authors.affiliation: National Technical University of Athens, Physics Department, Iroon Polytechneiou 9, 15780 Zografou, Athens, Greece.
authors.forename: Ioanna
authors.initials: I
authors.lastname: Zergioti
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D003300:Copper / Q000032:analysis*; D021881:DNA, Catalytic; D005453:Fluorescence; D007834:Lasers
chemical_list: D021881:DNA, Catalytic; D003300:Copper