title: Improving the fermentation quality of wheat straw silage stored at low temperature by psychrotrophic lactic acid bacteria.
abstract: This study aimed to explore the feasible approaches to develop a silage production technique in regions with low temperatures. An effective low-temperature silage technology system was constructed and two frigostable Lactobacillus (L.) strains isolated from alpine pastures were selected and proved to be available for wheat straw silage at 5°C. The strains QZ227 and QZ887 were both identified as L. plantarum according to the phenotype, 16S rRNA, and RecA gene analysis. QZ227, QZ887 and a commercial inoculant FG1 consisting of L. plantarum were effective for improving the fermentation quality of wheat straws silage at 5°C for 30 days as indicated by the higher content of lactic acid and for 60 days by lower pH values, while the control with sterile water instead conferred reduced benefits. Additionally, silages fermented at low temperature proved to be acceptable for feeding livestock after being placed in a simulated environmental temperature of 20°C for 14 days to detect its edibility during the early spring when the temperature begins to rise. Both QZ227 and QZ887 showed potential applications of silage making in frigid areas and were effective inoculants in a low-temperature silage technology system.
authors.affiliation: Henan Key Laboratory of Ion-Beam Bioengineering, College of Physics and Engineering, Zhengzhou University, Zhengzhou, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Henan Key Laboratory of Ion-Beam Bioengineering, College of Physics and Engineering, Zhengzhou University, Zhengzhou, China.
authors.forename: Haoxin
authors.initials: H
authors.lastname: Lv
authors.affiliation: Henan Key Laboratory of Ion-Beam Bioengineering, College of Physics and Engineering, Zhengzhou University, Zhengzhou, China.
authors.forename: Zhongfang
authors.initials: Z
authors.lastname: Tan
authors.affiliation: Henan Key Laboratory of Ion-Beam Bioengineering, College of Physics and Engineering, Zhengzhou University, Zhengzhou, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Li
authors.affiliation: Henan Key Laboratory of Ion-Beam Bioengineering, College of Physics and Engineering, Zhengzhou University, Zhengzhou, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Henan Key Laboratory of Ion-Beam Bioengineering, College of Physics and Engineering, Zhengzhou University, Zhengzhou, China.
authors.forename: Huili
authors.initials: H
authors.lastname: Pang
authors.affiliation: Henan Key Laboratory of Ion-Beam Bioengineering, College of Physics and Engineering, Zhengzhou University, Zhengzhou, China.
authors.forename: Zongwei
authors.initials: Z
authors.lastname: Li
authors.affiliation: Henan Key Laboratory of Ion-Beam Bioengineering, College of Physics and Engineering, Zhengzhou University, Zhengzhou, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Jiao
authors.affiliation: Zhejiang Academy of Agricultural Sciences, Institute of Crops and Utilization of Nuclear Technology, Hangzhou, China.
authors.forename: Qingsheng
authors.initials: Q
authors.lastname: Jin
mesh_terms: D003080:Cold Temperature; D005285:Fermentation; D063427:Food Quality; D061353:Food Storage; D048191:Lactiplantibacillus plantarum; D012820:Silage; D013997:Time Factors
keywords: lactic acid bacteria; low temperature; psychrotrophic; silage; temperature rising