title: Conversion of glycerol to 1,3-dihydroxyacetone by glycerol dehydrogenase co-expressed with an NADH oxidase for cofactor regeneration.
abstract: OBJECTIVE
  To investigate the efficiency of a cofactor regeneration enzyme co-expressed with a glycerol dehydrogenase for the production of 1,3-dihydroxyacetone (DHA).
  
  
  RESULTS
  In vitro biotransformation of glycerol was achieved with the cell-free extracts containing recombinant GlyDH (glycerol dehydrogenase from Escherichia coli), LDH (lactate dehydrogenase form Bacillus subtilis) or LpNox1 (NADH oxidase from Lactobacillus pentosus), giving DHA at 1.3 g l(-1) (GlyDH/LDH) and 2.2 g l(-1) (GlyDH/LpNox1) with total turnover number (TTN) of NAD(+) recycling of 6039 and 11100, respectively. Whole cells of E. coli (GlyDH-LpNox1) co-expressing both GlyDH and LpNox1 were constructed and converted 10 g glycerol l(-1) to DHA at 0.2-0.5 g l(-1) in the presence of zero to 2 mM exogenous NAD(+). The cell free extract of E. coli (GlyDH-LpNox) converted glycerol (2-50 g l(-1)) to DHA from 0.5 to 4.0 g l(-1) (8-25 % conversion) without exogenous NAD(+).
  
  
  CONCLUSIONS
  The disadvantage of the expensive consumption of NAD(+) for the production of DHA has been overcome.
authors.affiliation: Department of Biological and Pharmaceutical Engineering, College of Chemistry and Chemical Engineering, Taiyuan University of Technology, No.79 West Yingze Street, Taiyuan, 030024, Shanxi, People's Republic of China. zhangjiandong@tyut.edu.cn.
authors.forename: Jiandong
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Biological and Pharmaceutical Engineering, College of Chemistry and Chemical Engineering, Taiyuan University of Technology, No.79 West Yingze Street, Taiyuan, 030024, Shanxi, People's Republic of China.
authors.forename: Zhimei
authors.initials: Z
authors.lastname: Cui
authors.affiliation: Department of Biological and Pharmaceutical Engineering, College of Chemistry and Chemical Engineering, Taiyuan University of Technology, No.79 West Yingze Street, Taiyuan, 030024, Shanxi, People's Republic of China.
authors.forename: Honghong
authors.initials: H
authors.lastname: Chang
authors.affiliation: Department of Biological and Pharmaceutical Engineering, College of Chemistry and Chemical Engineering, Taiyuan University of Technology, No.79 West Yingze Street, Taiyuan, 030024, Shanxi, People's Republic of China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Fan
authors.affiliation: Department of Biological and Pharmaceutical Engineering, College of Chemistry and Chemical Engineering, Taiyuan University of Technology, No.79 West Yingze Street, Taiyuan, 030024, Shanxi, People's Republic of China.
authors.forename: Qiuyong
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Department of Biological and Pharmaceutical Engineering, College of Chemistry and Chemical Engineering, Taiyuan University of Technology, No.79 West Yingze Street, Taiyuan, 030024, Shanxi, People's Republic of China.
authors.forename: Wenlong
authors.initials: W
authors.lastname: Wei
mesh_terms: D004098:Dihydroxyacetone / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000378:metabolism*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D013401:Sugar Alcohol Dehydrogenases / Q000378:metabolism*
chemical_list: D009097:Multienzyme Complexes; D013401:Sugar Alcohol Dehydrogenases; C020672:glycerol dehydrogenase; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases; D004098:Dihydroxyacetone; D005990:Glycerol
keywords: 1,3-Dihydroxyacetone; Cofactor regeneration; Glycerol; Glycerol dehydrogenase; NADH oxidase