title: Outcomes of intracytoplasmic sperm injection in oligozoospermic men with Y chromosome AZFb or AZFc microdeletions.
abstract: We investigated whether the presence of Y chromosome azoospermia factor (AZF) microdeletions impacts upon the outcomes of intracytoplasmic sperm injection (ICSI) using fresh ejaculated spermatozoa. Sixteen oligozoospermia patients with Y chromosome AZFb or AZFc microdeletions and undergoing ICSI cycles between March 2013 and November 2014 were studied. Twenty-six infertile men with normal Y chromosomes and also undergoing IVF/ICSI in the same time period were used as controls. A retrospective case-control study approach was used. Among the 16 cases, 12 (75%, 12/16) had deletions of AZFc markers (sY152, sY254 and sY255), one (6.25%, 1/16) had a deletion of sY152, and two (12.5%, 2/16) had deletions of sY152, sY254, sY255 and sY157. AZFb microdeletions were found in one patient (6.25%, 1/16). There were no significant differences between groups for cleaved embryo rate, high-grade embryo rate, blastocyst formation rate, embryo implantation rate, clinical pregnancy rate and delivery rate. The clinical outcomes of ICSI for oligozoospermic patients with Y chromosome AZF microdeletion are comparable to those of infertile patients with normal Y chromosomes. Our findings indicate that ICSI should be offered to patients with an AZFc deletion and that oligozoospermia patients with AZFb microdeletions are likely to father children.
authors.affiliation: Center for Reproductive Medicine, Center of Prenatal Diagnosis, First Hospital, Jilin University, Changchun, China.
authors.forename: X-Y
authors.initials: XY
authors.lastname: Liu
authors.affiliation: Center for Reproductive Medicine, Center of Prenatal Diagnosis, First Hospital, Jilin University, Changchun, China.
authors.forename: R-X
authors.initials: RX
authors.lastname: Wang
authors.affiliation: Department of obstetrics and gynecology, First Hospital, Jilin University, Changchun, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Center for Reproductive Medicine, Center of Prenatal Diagnosis, First Hospital, Jilin University, Changchun, China.
authors.forename: L-L
authors.initials: LL
authors.lastname: Luo
authors.affiliation: Center for Reproductive Medicine, Center of Prenatal Diagnosis, First Hospital, Jilin University, Changchun, China.
authors.forename: W
authors.initials: W
authors.lastname: Guo
authors.affiliation: Center for Reproductive Medicine, Center of Prenatal Diagnosis, First Hospital, Jilin University, Changchun, China.
authors.forename: R-Z
authors.initials: RZ
authors.lastname: Liu
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D002872:Chromosome Deletion; D041322:Chromosomes, Human, Y / Q000235:genetics; D005260:Female; D006801:Humans; D007248:Infertility, Male / Q000235:genetics / Q000628:therapy*; D008297:Male; D009845:Oligospermia / Q000235:genetics / Q000628:therapy*; D011247:Pregnancy; D018873:Pregnancy Rate; D012189:Retrospective Studies; D012729:Sex Chromosome Aberrations; D058533:Sex Chromosome Disorders of Sex Development / Q000235:genetics / Q000628:therapy*; D020554:Sperm Injections, Intracytoplasmic; D016896:Treatment Outcome; D055815:Young Adult
keywords: AZFb microdeletion; AZFc microdeletion; intracytoplasmic sperm injection; oligozoospermia; sperm