title: Specific control of Arabidopsis BAK1/SERK4-regulated cell death by protein glycosylation.
abstract: Precise control of cell death is essential for the survival of all organisms. Arabidopsis thaliana BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1 (BAK1) and somatic embryogenesis receptor kinase 4 (SERK4) redundantly and negatively regulate cell death through elusive mechanisms. By deploying a genetic screen for suppressors of cell death triggered by virus-induced gene silencing of BAK1/SERK4 on Arabidopsis knockout collections, we identified STT3a, a protein involved in N-glycosylation modification, as an important regulator of bak1/serk4 cell death. Systematic investigation of glycosylation pathway and endoplasmic reticulum (ER) quality control (ERQC) components revealed distinct and overlapping mechanisms of cell death regulated by BAK1/SERK4 and their interacting protein BIR1. Genome-wide transcriptional analysis revealed the activation of members of cysteine-rich receptor-like kinase (CRK) genes in the bak1/serk4 mutant. Ectopic expression of CRK4 induced STT3a/N-glycosylation-dependent cell death in Arabidopsis and Nicotiana benthamiana. Therefore, N-glycosylation and specific ERQC components are essential to activate bak1/serk4 cell death, and CRK4 is likely to be among client proteins of protein glycosylation involved in BAK1/SERK4-regulated cell death.
authors.affiliation: Department of Biochemistry &Biophysics, Institute for Plant Genomics &Biotechnology, Texas A&M University, College Station, Texas 77843, USA.|Department of Plant Pathology &Microbiology, Institute for Plant Genomics &Biotechnology, Texas A&M University, College Station, Texas 77843, USA.
authors.forename: Marcos V V
authors.initials: MV
authors.lastname: de Oliveira
authors.affiliation: Department of Biochemistry &Biophysics, Institute for Plant Genomics &Biotechnology, Texas A&M University, College Station, Texas 77843, USA.|Molecular and Environmental Plant Sciences, Texas A&M University, College Station, Texas 77843, USA.
authors.forename: Guangyuan
authors.initials: G
authors.lastname: Xu
authors.affiliation: Department of Plant Pathology &Microbiology, Institute for Plant Genomics &Biotechnology, Texas A&M University, College Station, Texas 77843, USA.
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Plant Pathology &Microbiology, Institute for Plant Genomics &Biotechnology, Texas A&M University, College Station, Texas 77843, USA.|Center of Biosciences &Biotechnology, Darcy Ribeiro State University of North Rio de Janeiro, 28013-602 Brazil.
authors.forename: Luciano
authors.initials: L
authors.lastname: de Souza Vespoli
authors.affiliation: Department of Biochemistry &Biophysics, Institute for Plant Genomics &Biotechnology, Texas A&M University, College Station, Texas 77843, USA.
authors.forename: Xiangzong
authors.initials: X
authors.lastname: Meng
authors.affiliation: Department of Plant Pathology &Microbiology, Institute for Plant Genomics &Biotechnology, Texas A&M University, College Station, Texas 77843, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Plant Pathology &Microbiology, Institute for Plant Genomics &Biotechnology, Texas A&M University, College Station, Texas 77843, USA.
authors.forename: Xiao
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Plant Pathology &Microbiology, Institute for Plant Genomics &Biotechnology, Texas A&M University, College Station, Texas 77843, USA.|Center of Biosciences &Biotechnology, Darcy Ribeiro State University of North Rio de Janeiro, 28013-602 Brazil.
authors.forename: Suzane Ariádina
authors.initials: SA
authors.lastname: de Souza
authors.affiliation: Department of Plant Pathology &Microbiology, Institute for Plant Genomics &Biotechnology, Texas A&M University, College Station, Texas 77843, USA.|Center of Biosciences &Biotechnology, Darcy Ribeiro State University of North Rio de Janeiro, 28013-602 Brazil.
authors.forename: Aline C
authors.initials: AC
authors.lastname: Intorne
authors.affiliation: Department of Plant Pathology &Microbiology, Institute for Plant Genomics &Biotechnology, Texas A&M University, College Station, Texas 77843, USA.|Center of Biosciences &Biotechnology, Darcy Ribeiro State University of North Rio de Janeiro, 28013-602 Brazil.
authors.forename: Ana Marcia E
authors.initials: AM
authors.lastname: de A Manhães
authors.affiliation: Department of Plant Pathology &Microbiology, Institute for Plant Genomics &Biotechnology, Texas A&M University, College Station, Texas 77843, USA.|Medical Microbiology and Immunology Major, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
authors.forename: Abbey L
authors.initials: AL
authors.lastname: Musinsky
authors.affiliation: Molecular and Environmental Plant Sciences, Texas A&M University, College Station, Texas 77843, USA.|Department of Horticultural Sciences, Texas A&M University, College Station, Texas 77843, USA.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Koiwa
authors.affiliation: Center of Biosciences &Biotechnology, Darcy Ribeiro State University of North Rio de Janeiro, 28013-602 Brazil.
authors.forename: Gonçalo A
authors.initials: GA
authors.lastname: de Souza Filho
authors.affiliation: Department of Plant Pathology &Microbiology, Institute for Plant Genomics &Biotechnology, Texas A&M University, College Station, Texas 77843, USA.|Molecular and Environmental Plant Sciences, Texas A&M University, College Station, Texas 77843, USA.
authors.forename: Libo
authors.initials: L
authors.lastname: Shan
authors.affiliation: Department of Biochemistry &Biophysics, Institute for Plant Genomics &Biotechnology, Texas A&M University, College Station, Texas 77843, USA.|Molecular and Environmental Plant Sciences, Texas A&M University, College Station, Texas 77843, USA.
authors.forename: Ping
authors.initials: P
authors.lastname: He
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000201:enzymology / Q000235:genetics* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D016923:Cell Death; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D006031:Glycosylation; D009154:Mutation; D010641:Phenotype; D018515:Plant Leaves / Q000166:cytology / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D018517:Plant Roots / Q000166:cytology / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D036226:Seedlings / Q000166:cytology / Q000201:enzymology / Q000235:genetics / Q000502:physiology
chemical_list: D029681:Arabidopsis Proteins; D011494:Protein Kinases; C570517:SERK4 protein, Arabidopsis; C463660:BAK1 protein, Arabidopsis; C000626068:CRK4 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases