title: Different Gene Expression Patterns between Leaves and Flowers in Lonicera japonica Revealed by Transcriptome Analysis.
abstract: The perennial and evergreen twining vine, Lonicera japonica is an important herbal medicine with great economic value. However, gene expression information for flowers and leaves of L. japonica remains elusive, which greatly impedes functional genomics research on this species. In this study, transcriptome profiles from leaves and flowers of L. japonica were examined using next-generation sequencing technology. A total of 239.41 million clean reads were used for de novo assembly with Trinity software, which generated 150,523 unigenes with N50 containing 947 bp. All the unigenes were annotated using Nr, SwissProt, COGs (Clusters of Orthologous Groups), GO (Gene Ontology), and KEGG (Kyoto Encyclopedia of Genes and Genomes) databases. A total of 35,327 differentially expressed genes (DEGs, P ≤ 0.05) between leaves and flowers were detected. Among them, a total of 6602 DEGs were assigned with important biological processes including "Metabolic process," "Response to stimulus," "Cellular process," and etc. KEGG analysis showed that three possible enzymes involved in the biosynthesis of chlorogenic acid were up-regulated in flowers. Furthermore, the TF-based regulation network in L. japonica identified three differentially expressed transcription factors between leaves and flowers, suggesting distinct regulatory roles in L. japonica. Taken together, this study has provided a global picture of differential gene expression patterns between leaves and flowers in L japonica, providing a useful genomic resource that can also be used for functional genomics research on L. japonica in the future.
authors.affiliation: Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and TechnologyWuhan, China; Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Huanggang Normal UniversityHuanggang, China.
authors.forename: Libin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Institute of Biotechnology, Chinese Academy of Agricultural Sciences Beijing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Long
authors.affiliation: Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Fu
authors.affiliation: Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Huanggang Normal University Huanggang, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xiang
authors.affiliation: Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Huanggang Normal University Huanggang, China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Gan
authors.affiliation: Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Wu
authors.affiliation: Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Jia
authors.affiliation: Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.
authors.forename: Longjiang
authors.initials: L
authors.lastname: Yu
authors.affiliation: Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and TechnologyWuhan, China; Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Huanggang Normal UniversityHuanggang, China.
authors.forename: Maoteng
authors.initials: M
authors.lastname: Li
keywords: Lonicera japonica; differentially expressed genes (DEGs); network; transcription factors; transcriptome