title: Epidemiological and Virological Characterizations of the 2014 Dengue Outbreak in Guangzhou, China.
abstract: Dengue used to be recognized as an imported and sporadic disease in China. Since June 2014, an unexpected large dengue outbreak has attacked Guangzhou, China, resulting in more than 40,000 cases. Among the 1,942 laboratory-confirmed hospitalized dengue cases, 121 were diagnosed as severe dengue according to the 2009 WHO guideline, and 2 patients finally died. Laboratory diagnosis and virus isolation demonstrated that the majority (96%) cases were caused by dengue virus serotype 1 (DENV-1), and the others by serotype 2 (DENV-2). 14 DENV strains were isolated from the sera of acute-phase dengue patients during this outbreak, and the complete envelope (E) gene of 12 DENV-1 strains and two DENV-2 strains were determined using RT-PCR assay. Phylogenetic analysis based on the E gene revealed the DENV-1 strains isolated during the outbreak belonged to genotype I and V, respectively. These isolates formed three clades. DENV-2 isolates were assigned to the same clade belonging to genotype cosmopolitan. These strains isolated in 2014 were closely related to the isolates obtained from the same province, Guangdong, in 2013. No amino acid mutations known to increase virulence were identified throughout the E protein of isolates in 2014. These results indicate that dengue is turning into endemic in Guangdong, China, and extensive seroepidemiological investigation and mosquito control measures are critically needed in the future.
authors.affiliation: Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Guangzhou Eighth People's Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: Fu-Chun
authors.initials: FC
authors.lastname: Zhang
authors.affiliation: Guangzhou Eighth People's Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhu
authors.affiliation: Guangzhou Eighth People's Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.affiliation: Guangzhou Eighth People's Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: Wen-Xin
authors.initials: WX
authors.lastname: Hong
authors.affiliation: Guangzhou Eighth People's Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: Ling-Zhai
authors.initials: LZ
authors.lastname: Zhao
authors.affiliation: Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
authors.forename: Yong-Qiang
authors.initials: YQ
authors.lastname: Deng
authors.affiliation: Guangzhou Eighth People's Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Qiu
authors.affiliation: Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Guangzhou Eighth People's Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Cai
authors.affiliation: Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
authors.forename: Wu-Chun
authors.initials: WC
authors.lastname: Cao
authors.affiliation: Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China.
authors.forename: Cheng-Feng
authors.initials: CF
authors.lastname: Qin
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002681:China / Q000453:epidemiology; D003715:Dengue / Q000453:epidemiology*; D003716:Dengue Virus / Q000145:classification / Q000235:genetics / Q000472:pathogenicity; D004196:Disease Outbreaks / Q000706:statistics & numerical data; D005260:Female; D016679:Genome, Viral / Q000235:genetics; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D065288:Serogroup