title: Expression and clinical significance of miRNAs that may be associated with the FHIT gene in breast cancer.
abstract: The dysregulation of miRNA expression has frequently been observed in breast cancer. Therefore, we investigated the expression profile of miRNAs that may be associated with expression of the FHIT gene in breast cancer and assessed their clinicopathological significance. The expression levels of miR-143, miR-663a, miR-668, miR-922 and FHIT were analyzed in normal and malignant breast tissues from 65 patients with breast cancer. We studied the correlation between the expression of miR-143, miR-663a, miR-668, miR-922 and FHIT and the clinicopathological features presented by the patients. The expression levels of the miRNAs and FHIT were downregulated in breast cancer tissue. The expression levels of miR-143, miR-663a and miR-668 were significantly reduced in FHIT downregulated tumors. miR-668 expression was also significantly altered relative to FHIT down- and up- regulated tumor tissues. Reduced miR-663a expression was statistically associated with high-grade ER/PR (+) status, benign reactive hyperplasia, lymph-node metastasis, in-situ component >25% and Ki 67>15% compared with non-tumor tissues. Additionally, reduced miR-668 expression was significantly different between tumors with and without lymph-node metastasis. miR-668 may play an important role in breast cancer development and progression by regulating the expression of FHIT. Furthermore, miR-668 and miR-663a may be potential prognostic biomarkers for breast cancer.
authors.affiliation: Department of Medical Biology, Faculty of Medicine, University of Uludag, Bursa, Turkey.
authors.forename: Elif Demirdogen
authors.initials: ED
authors.lastname: Sevinc
authors.affiliation: Department of Medical Biology, Faculty of Medicine, University of Uludag, Bursa, Turkey. Electronic address: gcecener@uludag.edu.tr.
authors.forename: Gulsah
authors.initials: G
authors.lastname: Cecener
authors.affiliation: Department of Medical Biology, Faculty of Medicine, University of Uludag, Bursa, Turkey.
authors.forename: Secil
authors.initials: S
authors.lastname: Ak
authors.affiliation: Department of Medical Biology, Faculty of Medicine, University of Uludag, Bursa, Turkey.
authors.forename: Berrin
authors.initials: B
authors.lastname: Tunca
authors.affiliation: Department of Medical Biology, Faculty of Medicine, University of Uludag, Bursa, Turkey.
authors.forename: Unal
authors.initials: U
authors.lastname: Egeli
authors.affiliation: Department of General Surgery, Faculty of Medicine, University of Uludag, Bursa, Turkey.
authors.forename: Sehsuvar
authors.initials: S
authors.lastname: Gokgoz
authors.affiliation: Department of Pathology, Faculty of Medicine, University of Uludag, Bursa, Turkey.
authors.forename: Sahsine
authors.initials: S
authors.lastname: Tolunay
authors.affiliation: Department of General Surgery, Faculty of Medicine, University of Uludag, Bursa, Turkey.
authors.forename: Ismet
authors.initials: I
authors.lastname: Tasdelen
mesh_terms: D017766:Acid Anhydride Hydrolases / Q000235:genetics* / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D019295:Computational Biology; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D055815:Young Adult; D000099170:Bis(5'-adenosyl)triphosphatase
chemical_list: D017766:Acid Anhydride Hydrolases; D035683:MicroRNAs; D009363:Neoplasm Proteins; D000099170:Bis(5'-adenosyl)triphosphatase
keywords: Breast cancer; FHIT; miR-143; miR-663a; miR-668; miR-922