title: Changes in mast cell infiltration: a possible mechanism in detrusor overactivity induced by visceral hypersensitivity.
abstract: OBJECTIVE
  To establish the detrusor overactivity (DO) model induced by visceral hypersensitivity (VH) and investigate the relationship between mast cell (MC) infiltration and DO.
  
  
  METHODS
  Sixty rats are divided into 4 groups randomly: Group 1:Baseline group; Group 2: DO group; Group 3: CON group; Group 4: VH group. The colorectal distension (CRD) and abdominal withdral reflex (AWR) scores are performed to evaluate VH. The cystometric investigation and histological test of MC infiltration are assessed.
  
  
  RESULTS
  The threshold pressure of CRD in the VH group is significantly lower than that in the CON group (P<0.001). At the distension pressure ≥20 mmHg, the AWR scores of the VH group are significantly higher than those of the CON group (10 mmHg: P=0.33; 20 mmHg: P=0.028; 40 mmHg: P<0.001; 60 mmHg: P<0.001; 80 mmHg: P<0.001). DO model is successfully established in the VH group (DO rate=100%). Compared with the CON group, the numbers of MC infiltration are significantly increased in the VH group, including submucosa of bladder (P<0.001), mucosa lamina propria/mesentery of small intestine (P<0.001), and mucosa lamina propria/mesentery of large intestine (P<0.001). Furthermore, more MC activation as well as degranulation are observed in the VH group.
  
  
  CONCLUSIONS
  It is indicated that DO model can be established in the VH rats. The MC infiltration may play an important role in DO induced by VH, and may be helpful to understand the mechanisms of DO in VH patients.
authors.affiliation: Department of Urology, Qilu Hospital, Shandong University, Jinan, P.R. China.
authors.forename: Nian-Zhao
authors.initials: NZ
authors.lastname: Zhang
authors.affiliation: Department of Urology, Qilu Hospital, Shandong University, Jinan, P.R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Urology, Qilu Hospital, Shandong University, Jinan, P.R. China.
authors.forename: Chen
authors.initials: C
authors.lastname: Jun
authors.affiliation: Department of Biochemistry and Molecular Biology, Shandong University School of Medicine, Shandong University, Jinan, P.R. China.
authors.forename: Yan-Xia
authors.initials: YX
authors.lastname: Guo
authors.affiliation: Department of Biochemistry and Molecular Biology, Shandong University School of Medicine, Shandong University, Jinan, P.R. China.
authors.forename: Hui-Qing
authors.initials: HQ
authors.lastname: Yuan
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D006967:Hypersensitivity / Q000150:complications* / Q000473:pathology / Q000503:physiopathology*; D007422:Intestines / Q000473:pathology / Q000503:physiopathology; D043183:Irritable Bowel Syndrome / Q000150:complications / Q000473:pathology / Q000503:physiopathology; D008407:Mast Cells / Q000473:pathology*; D011312:Pressure; D011897:Random Allocation; D017208:Rats, Wistar; D015203:Reproducibility of Results; D053201:Urinary Bladder, Overactive / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology*; D014563:Urodynamics; D014781:Viscera / Q000473:pathology / Q000503:physiopathology*; D059265:Visceral Pain / Q000150:complications / Q000473:pathology / Q000503:physiopathology