title: Tissue localization of GM-CSF receptor in bovine ovarian follicles and its role on glucose uptake by mural granulosa cells.
abstract: The granulocyte-macrophage colony stimulating factor (GM-CSF) is a multifunctional cytokine implicated in proliferation, differentiation, and activation of several cell types including those involved in hematopoiesis and reproduction. In the present study, the expression of the α- and β-subunit genes of GM-CSF receptor during follicular development in cattle was assessed. The spatial association of α- and β-subunits of GM-CSF with follicle stimulating hormone receptor (FSHR) and 3β-hydroxysteroid dehydrogenase (3β-HSD), and the temporal associations with gene expression of hexose transporters (GLUTs) in granulosa cells of cattle were also evaluated. The effect of GM-CSF on the functionality of hexose transporters was also determined in an in vitro primary culture of granulosa cells. The spatial association of subunits of the GM-CSF receptor with 3β-HSD and FSHR suggests a potential steroidogenic regulation of GM-CSF in granulosa cells. Immunodetection of GLUTs and uptake kinetic assays confirmed expression and functionality of these genes for hexose transporters in granulosa cells of cattle. Treatment of granulosa cells with GM-CSF, FSH or insulin- like growth factor-I (IGF-I) alone increased 2-deoxyglucose (DOG) or 3-0-methylglucose (OMG) uptake; however, when cells were treated with various combination of these factors there were no additive effect. Unexpectedly, the combination of GM-CSF and FSH decreased DOG uptake compared to FSH treatment alone. Thus, the expression pattern of GM-CSF receptor subunit genes during follicle development in cattle and promotion of DOG and OMG uptake in granulosa cells indicate a role for GM-CSF, FSH and/or IGF-I alone in regulating granulosa cell metabolic activity, specifically by promoting glucose uptake.
authors.affiliation: Departamento de Fomento de la Producción Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Santiago, Chile.
authors.forename: O A
authors.initials: OA
authors.lastname: Peralta
authors.affiliation: Instituto de Bioquímica y Microbiología, Universidad Austral de Chile, Valdivia, Chile.
authors.forename: D
authors.initials: D
authors.lastname: Bucher
authors.affiliation: Instituto de Bioquímica y Microbiología, Universidad Austral de Chile, Valdivia, Chile.
authors.forename: C
authors.initials: C
authors.lastname: Angulo
authors.affiliation: Instituto de Bioquímica y Microbiología, Universidad Austral de Chile, Valdivia, Chile.
authors.forename: M A
authors.initials: MA
authors.lastname: Castro
authors.affiliation: Instituto de Ciencia Animal, Universidad Austral de Chile, Valdivia, Chile; Ross University School of Veterinary Medicine, St. Kitts, Basseterre, West Indies. Electronic address: mratto@rossvet.edu.kn.
authors.forename: M H
authors.initials: MH
authors.lastname: Ratto
authors.affiliation: Instituto de Bioquímica y Microbiología, Universidad Austral de Chile, Valdivia, Chile. Electronic address: conchagrabinger@uach.cl.
authors.forename: Il
authors.initials: I
authors.lastname: Concha
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000235:genetics / Q000378:metabolism; D019325:3-O-Methylglucose / Q000378:metabolism; D000818:Animals; D002417:Cattle / Q000502:physiology*; D003847:Deoxyglucose / Q000378:metabolism; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology; D005947:Glucose / Q000378:metabolism*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D006107:Granulosa Cells / Q000187:drug effects*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D009004:Monosaccharide Transport Proteins / Q000235:genetics / Q000378:metabolism; D006080:Ovarian Follicle / Q000378:metabolism*; D021122:Protein Subunits; D011849:Radioactive Tracers; D011962:Receptors, FSH / Q000235:genetics / Q000378:metabolism; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / Q000502:physiology*; D013997:Time Factors
chemical_list: D009004:Monosaccharide Transport Proteins; D021122:Protein Subunits; D011849:Radioactive Tracers; D011962:Receptors, FSH; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor; D019325:3-O-Methylglucose; D007334:Insulin-Like Growth Factor I; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D005640:Follicle Stimulating Hormone; D003847:Deoxyglucose; D015096:3-Hydroxysteroid Dehydrogenases; D005947:Glucose
keywords: Bovine follicle development; Cattle; Glucose transporters; Granulosa cells; Oocyte