title: Dissecting the role of transforming growth factor-β1 in topmouth culter immunobiological activity: a fundamental functional analysis.
abstract: Transforming growth factor-β1 (TGF-β1) has been proven to function primarily in mammalian immunobiological activity, but information regarding the immune role of TGF-β1 in teleosts is limited. In the present study, we describe the cDNA cloning and characterization of the TGF-β1 molecule in the topmouth culter. TGF-β1 is highly expressed in immune-related tissues of the culter, including the thymus, head kidney, and spleen. The recombinant culter TGF-β1 (cTGF-β1) was successfully expressed and purified in vitro, and the effects of cTGF-β1 on the mRNA expression of pro-inflammatory cytokines, such as TNF-α and IL-1β, in the absence or presence of LPS was determined in culter peripheral blood leukocytes. cTGF-β1 was found to have bipolar properties in inflammatory reactions. Additionally, to assess the immune role of teleost TGF-β1 in vivo, the expression of TGF-β1 in the culter thymus and spleen tissues induced by poly I:C were also examined. The expression of TGF-β1 was obviously up-regulated, as shown in the cell lines. However, the peak time of cTGF-β1 expression in the cell lines occurred significantly earlier than in the organic tissues under the same inducer, suggesting that the response of the teleost TGF-β1 molecule to exogenous infection depends on a more complicated signalling pathway in vivo than in vitro.
authors.affiliation: National Engineering Research Center of Marine Facilities Aquaculture, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan 316004, China.
authors.forename: Pengzhi
authors.initials: P
authors.lastname: Qi
authors.affiliation: College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Congxin
authors.initials: C
authors.lastname: Xie
authors.affiliation: National Engineering Research Center of Marine Facilities Aquaculture, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan 316004, China.
authors.forename: Baoying
authors.initials: B
authors.lastname: Guo
authors.affiliation: National Engineering Research Center of Marine Facilities Aquaculture, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan 316004, China.
authors.forename: Changwen
authors.initials: C
authors.lastname: Wu
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D003530:Cyprinidae / Q000235:genetics / Q000276:immunology*; D016207:Cytokines / Q000235:genetics; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D060226:Head Kidney / Q000378:metabolism; D011070:Poly I-C / Q000494:pharmacology; D015398:Signal Transduction; D013154:Spleen / Q000378:metabolism; D013950:Thymus Gland / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000235:genetics* / Q000378:metabolism*
chemical_list: D016207:Cytokines; D029941:Fish Proteins; D053773:Transforming Growth Factor beta1; D011070:Poly I-C