title: Evaluation of diagnostic value of AgNOR and PAP in early detection of dysplastic changes in leukoplakia and lichen planus - a preliminary case-control study.
abstract: OBJECTIVE
  Early detection of oral cancer has been the most effective approach to reduce morbidity and mortality of cancer patients. If a lesion is clinically considered suspicious, an easily practicable, non-invasive, painless, safe, and accurate screening method for detection of the dysplastic changes is necessary. In an attempt to procure this, a study was conducted with the aim of determining the diagnostic accuracy of rapid Papanicolaou stain (PAP) and silver-stained nucleolar organizer regions (AgNOR) in brush biopsies of potentially malignant lesions for early detection of oral cancer.
  
  
  METHODS
  Brush biopsies taken from 25 cases of leukoplakia and lichen planus each were stained with rapid PAP and silver nitrate stains. Histopathological correlation was performed and further compared with rapid PAP and AgNOR for its diagnostic validity.
  
  
  RESULTS
  Statistically significant increase in the mean AgNOR count was seen from normal epithelium to lichen planus to that of leukoplakia. When compared with rapid PAP, a linear correlation was seen in AgNOR counts and stages of dysplasia in leukoplakia which was also found to be statistically significant. Diagnostic accuracy for AgNOR in leukoplakia was found to be 84%, lichen planus 73%, whereas RAPID PAP showed 72% accuracy.
  
  
  CONCLUSIONS
  AgNOR analysis may be useful as a quantitative marker of incipient cellular alterations and hence would be helpful in assessing suspicious lesions and thus can be regarded as a valuable adjunct.
authors.affiliation: Oral Medicine and Radiology, A J institute of Dental Sciences, Mangalore, Karnataka, India.
authors.forename: Dhanya S
authors.initials: DS
authors.lastname: Rao
authors.affiliation: Oral Medicine and Radiology, College of Dental Sciences, Davangere, Karnataka, India.
authors.forename: I M
authors.initials: IM
authors.lastname: Ali
authors.affiliation: Oral Medicine and Radiology, College of Dental Sciences, Davangere, Karnataka, India.
authors.forename: Rajeshwari G
authors.initials: RG
authors.lastname: Annigeri
mesh_terms: D000704:Analysis of Variance; D034961:Antigens, Nuclear / Q000032:analysis*; D001706:Biopsy / Q000379:methods; D016022:Case-Control Studies; D004396:Coloring Agents; D005260:Female; D006801:Humans; D007972:Leukoplakia, Oral / Q000473:pathology*; D017676:Lichen Planus, Oral / Q000473:pathology*; D008297:Male; D009061:Mouth Mucosa / Q000473:pathology; D009062:Mouth Neoplasms / Q000175:diagnosis* / Q000473:pathology; D065006:Papanicolaou Test; D011230:Precancerous Conditions / Q000473:pathology*; D012834:Silver
chemical_list: D034961:Antigens, Nuclear; D004396:Coloring Agents; C051963:nucleolar organizer region associated proteins; D012834:Silver
keywords: AgNOR; dysplasia; oral leukoplakia; oral lichen Planus; rapid PAP