title: Delivery of Synthetic mRNA Encoding FOXP3 Antigen into Dendritic Cells for Inflammatory Breast Cancer Immunotherapy.
abstract: Dendritic cell (DC)-based vaccines are commonly used for cancer immunotherapy. To prepare vaccines, DCs are pulsed or transfected with either: (a) defined peptides of tumor-associated antigens, (b) total protein isolated from the tumor cell, (c) autologous total RNA isolated from the tumor cell, (d) synthetic tumor-antigen-encoding mRNA, or (e) genes that encode for specific tumor-associated antigens. Introduction of tumor-associated antigen(s) and subsequent generation of mature DCs that can stimulate tumor-antigen-specific cytotoxic T lymphocytes comprise the critical steps of cancer vaccine preparation. Here, we described a method of: (a) preparing and delivering synthetic FOXP3 mRNA into human DCs, (b) generating mature DCs,
authors.affiliation: Division of Surgical Sciences, Department of Surgery, Women's Cancer Program, Duke Cancer Institute, Duke University School of Medicine, Durham, NC, 27710, USA. gayathri.devi@duke.edu.
authors.forename: Gayathri R
authors.initials: GR
authors.lastname: Devi
authors.affiliation: Division of Surgical Sciences, Department of Surgery, Women's Cancer Program, Duke Cancer Institute, Duke University School of Medicine, Durham, NC, 27710, USA.
authors.forename: Sritama
authors.initials: S
authors.lastname: Nath
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics / Q000276:immunology; D019496:Cancer Vaccines / Q000276:immunology; D045744:Cell Line, Tumor; D064987:Cell- and Tissue-Based Therapy; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D018274:Electroporation; D005260:Female; D051858:Forkhead Transcription Factors / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007167:Immunotherapy; D058922:Inflammatory Breast Neoplasms / Q000235:genetics / Q000276:immunology* / Q000628:therapy; D012333:RNA, Messenger / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology* / Q000378:metabolism; D014162:Transfection
chemical_list: D000951:Antigens, Neoplasm; D019496:Cancer Vaccines; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D012333:RNA, Messenger
keywords: Cancer vaccines; Cytotoxic T lymphocyte (CTL) assay; Dendritic cell; Electroporation; Lipofection; Synthetic mRNA; Tumor-associated antigens