title: Reactive oxygen species acts as executor in radiation enhancement and autophagy inducing by AgNPs.
abstract: Malignant glioma is one of the most common intracranial tumor with a dismal prognosis. The radiosensitizing effect of silver nanoparticles (AgNPs) on glioma both in vitro and in vivo were demonstrated in the previous studies of our group. However, the underlying mechanism is still unclear. In this present study, the use of antioxidants is employed for the regulating of reactive oxygen species (ROS) in U251 cells treated with various agents, and the results shows that ROS played an essential role in the autophagy inducing and radiosensitization effect of AgNPs. Moreover, the inhibition of protective autophagy with 3-MA is another way to increase ROS, resulting in the increasing of cell death and apoptosis. Taken together, understanding the relationship between the elevated ROS and autophagy and the effect of ROS should be useful to the clinical applications of AgNPs. These findings could potentially be exploited for new therapeutic strategies in glioma radiotherapy.
authors.affiliation: State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.affiliation: Hefei National Laboratory for Physical Sciences at the Microscale, School of Life Sciences, University of Science and Technology of China, Hefei 230027, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China; Institute of Neurobiology, School of Medicine, Southeast University, Nanjing 210009, PR China.
authors.forename: Peidang
authors.initials: P
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China.
authors.forename: Zhihai
authors.initials: Z
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
authors.affiliation: Institute of Neurobiology, School of Medicine, Southeast University, Nanjing 210009, PR China.
authors.forename: Haizhen
authors.initials: H
authors.lastname: Jin
authors.affiliation: Traditional Chinese Medicine Hospital of Jiangsu Province, Nanjing 210009, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ma
authors.affiliation: Hefei National Laboratory for Physical Sciences at the Microscale, School of Life Sciences, University of Science and Technology of China, Hefei 230027, PR China. Electronic address: lpwen@ustc.edu.cn.
authors.forename: Longping
authors.initials: L
authors.lastname: Wen
authors.affiliation: State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China. Electronic address: guning@seu.edu.cn.
authors.forename: Ning
authors.initials: N
authors.lastname: Gu
mesh_terms: D001343:Autophagy / Q000187:drug effects / Q000528:radiation effects; D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology / Q000532:radiotherapy*; D045744:Cell Line, Tumor; D005910:Glioma / Q000378:metabolism / Q000473:pathology / Q000532:radiotherapy*; D006801:Humans; D053768:Metal Nanoparticles / Q000627:therapeutic use* / Q000648:ultrastructure; D011838:Radiation-Sensitizing Agents / Q000627:therapeutic use*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012834:Silver / Q000627:therapeutic use*
chemical_list: D011838:Radiation-Sensitizing Agents; D017382:Reactive Oxygen Species; D012834:Silver
keywords: Autophagy; Glioma; Radiation sensitization; Radiotherapy; Reactive oxygen species (ROS); Silver nanoparticles