title: YUCCA-mediated auxin biogenesis is required for cell fate transition occurring during de novo root organogenesis in Arabidopsis.
abstract: Many plant organs have the ability to regenerate a new plant after detachment or wounding via de novo organogenesis. During de novo root organogenesis from Arabidopsis thaliana leaf explants, endogenic auxin is essential for the fate transition of regeneration-competent cells to become root founder cells via activation of WUSCHEL-RELATED HOMEOBOX 11 (WOX11). However, the molecular events from leaf explant detachment to auxin-mediated cell fate transition are poorly understood. In this study, we used an assay to determine the concentration of indole-3-acetic acid (IAA) to provide direct evidence that auxin is produced after leaf explant detachment, a process that involves YUCCA (YUC)-mediated auxin biogenesis. Inhibition of YUC prevents expression of WOX11 and fate transition of competent cells, resulting in the blocking of rooting. Further analysis showed that YUC1 and YUC4 act quickly (within 4 hours) in response to wounding after detachment in both light and dark conditions and promote auxin biogenesis in both mesophyll and competent cells, whereas YUC5, YUC8, and YUC9 primarily respond in dark conditions. In addition, YUC2 and YUC6 contribute to rooting by providing a basal auxin level in the leaf. Overall, our study indicates that YUC genes exhibit a division of labour during de novo root organogenesis from leaf explants in response to multiple signals.
authors.affiliation: National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China.
authors.forename: Lyuqin
authors.initials: L
authors.lastname: Chen
authors.affiliation: Pre-National Laboratory for Crop Germplasm Innovation and Resource Utilization, Hunan Agricultural University, Changsha, Hunan 410128, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Tong
authors.affiliation: Pre-National Laboratory for Crop Germplasm Innovation and Resource Utilization, Hunan Agricultural University, Changsha, Hunan 410128, China.
authors.forename: Langtao
authors.initials: L
authors.lastname: Xiao
authors.affiliation: Pre-National Laboratory for Crop Germplasm Innovation and Resource Utilization, Hunan Agricultural University, Changsha, Hunan 410128, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Ruan
authors.affiliation: National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, China.
authors.forename: Jingchun
authors.initials: J
authors.lastname: Liu
authors.affiliation: National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China.
authors.forename: Minhuan
authors.initials: M
authors.lastname: Zeng
authors.affiliation: National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Huang
authors.affiliation: National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China.
authors.forename: Jia-Wei
authors.initials: JW
authors.lastname: Wang
authors.affiliation: National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China xulin01@sibs.ac.cn.
authors.forename: Lin
authors.identifier: 0000-0003-4718-1286
authors.initials: L
authors.lastname: Xu
mesh_terms: D017360:Arabidopsis / Q000254:growth & development* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology; D003577:Cytochrome P-450 Enzyme System / Q000502:physiology; D007210:Indoleacetic Acids / Q000378:metabolism*; D010105:Oxygenases / Q000502:physiology*; D010937:Plant Growth Regulators / Q000378:metabolism / Q000502:physiology*; D018515:Plant Leaves / Q000254:growth & development; D018517:Plant Roots / Q000254:growth & development*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; D003577:Cytochrome P-450 Enzyme System; C000609125:YUC5 protein, Arabidopsis; D010105:Oxygenases; C422746:YUC protein, Arabidopsis
keywords: Arabidopsis; WOX11; YUCCA.; auxin biogenesis; de novo root organogenesis; plant regeneration