title: Doxorubicin loaded large-pore mesoporous hydroxyapatite coated superparamagnetic Fe3O4 nanoparticles for cancer treatment.
abstract: In the present study, a series of multifunctional drug delivery systems based on mesostructured hydroxyapatite coating and superparamagnetic nanoparticles with pH-responsive characters was prepared. The structure of each new synthesized nanoscale composite was fully characterized by XRD, FTIR, TEM, VSM and BET. The results showed a good ordered mesostructure having large pores, high pore volume, high surface area, and varied super paramagnetic properties. The mesoporous hydroxyapatite coated super paramagnetic Fe3O4 nanoparticles were applied as a drug delivery carrier loaded with doxorubicin (DOX) as a model drug. The storage/release properties of the developed nonocarriers in phosphate buffer saline (PBS) were studied in two certain pHs: pH=7.4 (the human blood pH) and pH=5.5 (pH of cancer cells). The large pores in the synthesized mesoporous acted as an excellent carrier for DOX molecules with a loading efficiency of ≈93% which is much higher than that of the conventional hydroxyapatite particles. When the pH of the release medium (PBS) was changed from 7.4 to 5.5, the drug release increased significantly from 10% of the adsorbed drug to about 70%. DOX-loaded mesostructure hydroxyapatite reduced the viability of SKBR3 and T47D cells by 54.7 and 57.3%, respectively, which were very similar to 56.8 and 60.4% reduction resulted from free DOX incubation. This new drug delivery system which benefits from both super paramagnetic properties and pH-responsive performances may serve as a suitable platform for developing new biocompatible drug carriers and could have a good potential use in targeted cancer therapy.
authors.affiliation: School of Metallurgy and Materials Engineering, Iran University of Science and Technology, Tehran 16846-13114, Iran; Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, 51664-14766 Tabriz- Iran.
authors.forename: Negar
authors.initials: N
authors.lastname: Abbasi Aval
authors.affiliation: Department of Medical Physics, School of Medicine, Tabriz University of Medical Sciences, Tabriz 51664-14766, Iran.
authors.forename: Jalil
authors.initials: J
authors.lastname: Pirayesh Islamian
authors.affiliation: Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, 51664-14766 Tabriz- Iran; Department of Medical Physics, School of Medicine, Tabriz University of Medical Sciences, Tabriz 51664-14766, Iran.
authors.forename: Milad
authors.initials: M
authors.lastname: Hatamian
authors.affiliation: Biotechnology Research Laboratory, School of Chemical Engineering, Iran University of Science and Technology, Tehran 16846-13114, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Arabfirouzjaei
authors.affiliation: School of Metallurgy and Materials Engineering, Iran University of Science and Technology, Tehran 16846-13114, Iran.
authors.forename: Jafar
authors.initials: J
authors.lastname: Javadpour
authors.affiliation: Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, 51664-14766 Tabriz- Iran; School of Pharmacy, Tabriz university of medical sciences, Tabriz 51664-14766, Iran. Electronic address: rashidi@tbzmed.ac.ir.
authors.forename: Mohammad-Reza
authors.initials: MR
authors.lastname: Rashidi
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004317:Doxorubicin / Q000008:administration & dosage* / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods; D065546:Drug Liberation; D017886:Durapatite / Q000737:chemistry*; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D009369:Neoplasms / Q000188:drug therapy*; D016062:Porosity
chemical_list: D004337:Drug Carriers; D058185:Magnetite Nanoparticles; D004317:Doxorubicin; D017886:Durapatite; D052203:Ferrosoferric Oxide
keywords: Cancer treatment; Doxorubicin; Drug delivery system; Mesoporous hydroxyapatite; Superparamagnetic iron oxide nanoparticles