title: 17β-Estradiol Protects Skeletal Myoblasts From Apoptosis Through p53, Bcl-2, and FoxO Families.
abstract: 17β-Estradiol (E2) protects several nonreproductive tissues from apoptosis, including skeletal muscle. Previously, we showed that E2at physiological concentrations prevented apoptosis induced by H2O2in skeletal myoblasts, reverting PKCδ, JNK, and p66Shc activation and exerting a beneficial action over mitochondria. Since genomic actions underlying the regulation of nuclear gene transcription are a common property of this steroid, the present work characterizes the transcriptional activity modulated by E2to exert its antiapoptotic effect. We report that E2protects skeletal myoblasts against apoptosis induced by H2O2modulating p53 and FoxO transcription factors and then their target genes Bcl-2, Bim, Puma, PERP, and MDM2, without affecting Noxa gene. The results presented in this work support the notion that the transcription factors FoxO and p53 coordinate apoptosis in C2C12 cells, and deepens our knowledge about a putative molecular mechanism by which E2exerts beneficial effects against oxidative stress in skeletal myoblasts. J. Cell. Biochem. 118: 104-115, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: INBIOSUR-CONICET, Bahía Blanca, 8000, Argentina.
authors.forename: Anabela
authors.initials: A
authors.lastname: La Colla
authors.affiliation: INBIOSUR-CONICET, Bahía Blanca, 8000, Argentina.
authors.forename: Andrea
authors.initials: A
authors.lastname: Vasconsuelo
authors.affiliation: INBIOSUR-CONICET, Bahía Blanca, 8000, Argentina.
authors.forename: Lorena
authors.initials: L
authors.lastname: Milanesi
authors.affiliation: INBIOSUR-CONICET, Bahía Blanca, 8000, Argentina.
authors.forename: Lucía
authors.initials: L
authors.lastname: Pronsato
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D004958:Estradiol / Q000494:pharmacology*; D051858:Forkhead Transcription Factors / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D051379:Mice; D032448:Myoblasts, Skeletal / Q000166:cytology / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D051858:Forkhead Transcription Factors; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53; C052617:Bcl2 protein, mouse; D004958:Estradiol; D006861:Hydrogen Peroxide
keywords: 17β-ESTRADIOL; Bcl-2; C2C12 MUSCLE CELLS; FoxOs; p53