title: Sirtuin 6 (SIRT6) Activity Assays.
abstract: SIRT6 has been shown to possess weak deacetylation, mono-ADP-ribosyltransferase activity, and deacylation activity in vitro. SIRT6 selectively deacetylates H3K9Ac and H3K56Ac. Several SIRT6 assays have been developed including HPLC assays, fluorogenic assays, FRET, magnetic beads, in silico, and bioaffinity chromatography assays. Herein, we describe detailed protocols for the HPLC based activity/inhibition assays, magnetic beads deacetylation assays, bioaffinity chromatographic assays as well as fluorogenic and in silico assays.
authors.affiliation: Bioanalytical and Drug Development Unit, National institute on Aging, National Institutes of Health, 251 Bayview Boulevard, Suite 100, Baltimore, MD, 21224-6825, USA.|School of Pharmacy, University of Eastern Finland, Kuopio, 70210, Finland.
authors.forename: Minna
authors.initials: M
authors.lastname: Rahnasto-Rilla
authors.affiliation: School of Pharmacy, University of Eastern Finland, Kuopio, 70210, Finland.
authors.forename: Maija
authors.initials: M
authors.lastname: Lahtela-Kakkonen
authors.affiliation: Bioanalytical and Drug Development Unit, National institute on Aging, National Institutes of Health, 251 Bayview Boulevard, Suite 100, Baltimore, MD, 21224-6825, USA. moaddelru@grc.nia.nih.gov.
authors.forename: Ruin
authors.initials: R
authors.lastname: Moaddel
mesh_terms: D000107:Acetylation; D002851:Chromatography, High Pressure Liquid; D003198:Computer Simulation; D031541:Fluorescence Resonance Energy Transfer; D006657:Histones / Q000378:metabolism; D006801:Humans; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D037761:Sirtuins / Q000378:metabolism*
chemical_list: D006657:Histones; C516592:SIRT6 protein, human; D037761:Sirtuins
keywords: Activation assays; Bioaffinity chromatography; Deacetylation assays; In silico screening; Inhibition assays