title: From dirt to industrial applications: Pseudomonas putida as a Synthetic Biology chassis for hosting harsh biochemical reactions.
abstract: The soil bacterium Pseudomonas putida is endowed with a central carbon metabolic network capable of fulfilling high demands of reducing power. This situation arises from a unique metabolic architecture that encompasses the partial recycling of triose phosphates to hexose phosphates-the so-called EDEMP cycle. In this article, the value of P. putida as a bacterial chassis of choice for contemporary, industrially-oriented metabolic engineering is addressed. The biochemical properties that make this bacterium adequate for hosting biotransformations involving redox reactions as well as toxic compounds and intermediates are discussed. Finally, novel developments and open questions in the continuous quest for an optimal microbial cell factory are presented at the light of current and future needs in the area of biocatalysis.
authors.affiliation: Systems and Synthetic Biology Program, Centro Nacional de Biotecnología (CNB-CSIC), 28049 Madrid, Spain. Electronic address: pablo.nikel@cnb.csic.es.
authors.forename: Pablo I
authors.initials: PI
authors.lastname: Nikel
authors.affiliation: Escuela de Química & CIPRONA, Universidad de Costa Rica, 11501-2060 San José, Costa Rica.
authors.forename: Max
authors.initials: M
authors.lastname: Chavarría
authors.affiliation: AMAbiotics SAS, Institut of Cardiometabolism and Nutrition (ICAN), Hôpital Universitaire de la Pitié-Salpêtrière, 75013 Paris, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Danchin
authors.affiliation: Systems and Synthetic Biology Program, Centro Nacional de Biotecnología (CNB-CSIC), 28049 Madrid, Spain. Electronic address: vdlorenzo@cnb.csic.es.
authors.forename: Víctor
authors.initials: V
authors.lastname: de Lorenzo
mesh_terms: D002244:Carbon / Q000378:metabolism; D060847:Metabolic Engineering; D016958:Pseudomonas putida / Q000378:metabolism*; D058615:Synthetic Biology
chemical_list: D002244:Carbon