title: MALDI-MSI for the analysis of a 3D tissue-engineered psoriatic skin model.
abstract: MALDI-MS Imaging is a novel label-free technique that can be used to visualize the changes in multiple mass responses following treatment. Following treatment with proinflammatory cytokine interleukin-22 (IL-22), the epidermal differentiation of Labskin, a living skin equivalent (LSE), successfully modeled psoriasis in vitro. Masson's trichrome staining enabled visualization and quantification of epidermal differentiation between the untreated and IL-22 treated psoriatic LSEs. Matrix-assisted laser desorption ionization mass spectrometry imaging was used to observe the spatial location of the psoriatic therapy drug acetretin following 48 h treatments within both psoriatic and normal LSEs. After 24 h, the drug was primarily located in the epidermal regions of both the psoriatic and nonpsoriatic LSE models whereas after 48 h it was detectible in the dermis.
authors.affiliation: Centre for Mass Spectrometry Imaging, Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, UK.
authors.forename: Amanda
authors.initials: A
authors.lastname: Harvey
authors.affiliation: Centre for Mass Spectrometry Imaging, Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, UK.
authors.forename: Laura M
authors.initials: LM
authors.lastname: Cole
authors.affiliation: Centre for Mass Spectrometry Imaging, Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, UK.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Day
authors.affiliation: Innovenn, Sand Hutton Innovation Campus, York, UK.
authors.forename: Maggie
authors.initials: M
authors.lastname: Bartlett
authors.affiliation: Innovenn, Sand Hutton Innovation Campus, York, UK.
authors.forename: John
authors.initials: J
authors.lastname: Warwick
authors.affiliation: Innovenn, Sand Hutton Innovation Campus, York, UK.
authors.forename: Richard
authors.initials: R
authors.lastname: Bojar
authors.affiliation: Centre for Mass Spectrometry Imaging, Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, UK.
authors.forename: David
authors.initials: D
authors.lastname: Smith
authors.affiliation: Centre for Mass Spectrometry Imaging, Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, UK.
authors.forename: Neil
authors.initials: N
authors.lastname: Cross
authors.affiliation: Centre for Mass Spectrometry Imaging, Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, UK.
authors.forename: Malcolm R
authors.initials: MR
authors.lastname: Clench
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D004195:Disease Models, Animal; D004817:Epidermis / Q000187:drug effects / Q000648:ultrastructure*; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods; D007378:Interleukins / Q000008:administration & dosage; D051379:Mice; D011565:Psoriasis / Q000235:genetics* / Q000473:pathology; D012867:Skin / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D023822:Tissue Engineering / Q000379:methods; D000097043:Interleukin-22
chemical_list: D007378:Interleukins; D000097043:Interleukin-22
keywords: Acetretin; Interleukin-22; Living skin equivalent; MALDI-MSI; Psoriasis; Skin; Technology; Tissue engineering