title: A Sensitive IHC Method for Monitoring Autophagy-Specific Markers in Human Tumor Xenografts.
abstract: Objective. Use of tyramide signal amplification (TSA) to detect autophagy biomarkers in formalin fixed and paraffin embedded (FFPE) xenograft tissue. Materials and Methods. Autophagy marker regulation was studied in xenograft tissues using Amp HQ IHC and standard IHC methods. Results. The data demonstrate the feasibility of using high sensitivity TSA IHC assays to measure low abundant autophagy markers in FFPE xenograft tissue.
authors.affiliation: Department of Molecular Pathology, Takeda Pharmaceuticals International Co., Cambridge, MA 02139, USA.
authors.forename: Helen
authors.initials: H
authors.lastname: He
authors.affiliation: Department of Molecular Pathology, Takeda Pharmaceuticals International Co., Cambridge, MA 02139, USA.
authors.forename: Yu
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Molecular Pathology, Takeda Pharmaceuticals International Co., Cambridge, MA 02139, USA.
authors.forename: Zhongmin
authors.initials: Z
authors.lastname: Xiang
authors.affiliation: Department of Molecular Pathology, Takeda Pharmaceuticals International Co., Cambridge, MA 02139, USA.
authors.forename: Lunyin
authors.initials: L
authors.lastname: Yu
authors.affiliation: Cancer Pharmacology, Takeda Pharmaceuticals International Co., Cambridge, MA 02139, USA.
authors.forename: Jouhara
authors.initials: J
authors.lastname: Chouitar
authors.affiliation: Cancer Pharmacology, Takeda Pharmaceuticals International Co., Cambridge, MA 02139, USA.
authors.forename: Jie
authors.initials: J
authors.lastname: Yu
authors.affiliation: Early Discovery, Takeda Pharmaceuticals International Co., Cambridge, MA 02139, USA.
authors.forename: Natalie Roy
authors.initials: NR
authors.lastname: D'Amore
authors.affiliation: Protein Sciences, Takeda Pharmaceuticals International Co., Cambridge, MA 02139, USA.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.affiliation: Protein Sciences, Takeda Pharmaceuticals International Co., Cambridge, MA 02139, USA.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Molecular Pathology, Takeda Pharmaceuticals International Co., Cambridge, MA 02139, USA.
authors.forename: Douglas
authors.initials: D
authors.lastname: Bowman
authors.affiliation: Department of Molecular Pathology, Takeda Pharmaceuticals International Co., Cambridge, MA 02139, USA.
authors.forename: Matthew
authors.initials: M
authors.lastname: Theisen
authors.affiliation: Early Discovery, Takeda Pharmaceuticals International Co., Cambridge, MA 02139, USA.
authors.forename: James E
authors.initials: JE
authors.lastname: Brownell
authors.affiliation: Department of Molecular Pathology, Takeda Pharmaceuticals International Co., Cambridge, MA 02139, USA.
authors.forename: Stephen
authors.initials: S
authors.lastname: Tirrell