title: Quantitative proteomics and phosphoproteomics of sugar beet monosomic addition line M14 in response to salt stress.
abstract: Salinity is a major abiotic stress affecting plant growth, development and agriculture productivity. Understanding the molecular mechanisms of salt stress tolerance will provide valuable information for effective crop engineering and breeding. Sugar beet monosomic addition line M14 obtained from the intercross between Beta vulgaris L. and Beta corolliflora Zoss exhibits tolerance to salt stress. In this study, the changes in the M14 proteome and phosphoproteome induced by salt stress were analyzed. We report the characteristics of the M14 plants under 0, 200, and 400mM NaCl using label-free quantitative proteomics approaches. Protein samples were subjected to total proteome profiling using LC-MS/MS and phosphopeptide enrichment to identify phosphopeptides and phosphoproteins. A total of 2182 proteins were identified and 114 proteins showed differential levels under salt stress. Interestingly, 189 phosphoproteins exhibited significant changes at the phosphorylation level under salt stress. Several signaling components associated with salt stress were found, e.g. 14-3-3 and mitogen-activated protein kinases (MAPK). Fifteen differential phosphoproteins and proteins involved in signal transduction were tested at the transcriptional level. The results revealed the short-term salt responsive mechanisms of the special sugar beet M14 line using label-free quantitative phosphoproteomics.
  
  
  
  Sugar beet monosomic addition line M14 is a special germplasm with salt stress tolerance. Analysis of the M14 proteome and phosphoproteome under salt stress has provided insight into specific response mechanisms underlying salt stress tolerance. Reversible protein phosphorylation regulates a wide range of cellular processes such as transmembrane signaling, intracellular amplification of signals, and cell-cycle control. This study has identified significantly changed proteins and phosphoproteins, and determined their potential relevance to salt stress response. The knowledge gained can be potentially applied to improving crop salt tolerance.
authors.affiliation: Key Laboratory of Molecular Biology of Heilongjiang Province, College of Life Sciences, Heilongjiang University, Harbin 150080, China; Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150080, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Yu
authors.affiliation: Key Laboratory of Molecular Biology of Heilongjiang Province, College of Life Sciences, Heilongjiang University, Harbin 150080, China.
authors.forename: Jinna
authors.initials: J
authors.lastname: Li
authors.affiliation: Proteomics and Mass Spectrometry, Interdisciplinary Center for Biotechnology Research, University of Florida, FL 32610, USA.
authors.forename: Jin
authors.initials: J
authors.lastname: Koh
authors.affiliation: Thermo Fisher Scientific, 1400 Northpoint Parkway, West Palm Beach, FL 33407, USA.
authors.forename: Craig
authors.initials: C
authors.lastname: Dufresne
authors.affiliation: Key Laboratory of Molecular Biology of Heilongjiang Province, College of Life Sciences, Heilongjiang University, Harbin 150080, China.
authors.forename: Na
authors.initials: N
authors.lastname: Yang
authors.affiliation: Key Laboratory of Molecular Biology of Heilongjiang Province, College of Life Sciences, Heilongjiang University, Harbin 150080, China.
authors.forename: Shishi
authors.initials: S
authors.lastname: Qi
authors.affiliation: Key Laboratory of Molecular Biology of Heilongjiang Province, College of Life Sciences, Heilongjiang University, Harbin 150080, China.
authors.forename: Yongxue
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Molecular Biology of Heilongjiang Province, College of Life Sciences, Heilongjiang University, Harbin 150080, China; Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150080, China.
authors.forename: Chunquan
authors.initials: C
authors.lastname: Ma
authors.affiliation: Department of Biology, Genetics Institute, Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL 32610, USA.
authors.forename: Benjamin V
authors.initials: BV
authors.lastname: Duong
authors.affiliation: Key Laboratory of Molecular Biology of Heilongjiang Province, College of Life Sciences, Heilongjiang University, Harbin 150080, China; Proteomics and Mass Spectrometry, Interdisciplinary Center for Biotechnology Research, University of Florida, FL 32610, USA; Department of Biology, Genetics Institute, Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL 32610, USA.
authors.forename: Sixue
authors.initials: S
authors.lastname: Chen
authors.affiliation: Key Laboratory of Molecular Biology of Heilongjiang Province, College of Life Sciences, Heilongjiang University, Harbin 150080, China; Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150080, China. Electronic address: lvzh3000@sina.com.
authors.forename: Haiying
authors.initials: H
authors.lastname: Li
mesh_terms: D027461:Beta vulgaris / Q000737:chemistry* / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D010750:Phosphoproteins / Q000032:analysis* / Q000502:physiology; D010766:Phosphorylation / Q000187:drug effects; D040901:Proteomics / Q000379:methods; D054712:Salinity; D055049:Salt Tolerance; D015398:Signal Transduction; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological / Q000187:drug effects
chemical_list: D010750:Phosphoproteins; D012965:Sodium Chloride
keywords: Label-free; Molecular mechanisms; Phosphoproteomics; Proteomics; Salt stress; Sugar beet M14