title: Synthesis and application of molecularly imprinted nanoparticles combined ultrasonic assisted for highly selective solid phase extraction trace amount of celecoxib from human plasma samples using design expert (DXB) software.
abstract: In this work molecular imprinted nanoparticles (MINPs) was synthesized and applied for ultrasonic assisted solid phase extraction of celecoxib (CEL) from human plasma sample following its combination by HPLC-UV. The MINPs were prepared in a non-covalent approach using methacrylic acid as monomer, CEL as template, ethylene glycol dimethacrylate as cross-linker, and 2,2-azobisisobutyronitrile (AIBN) as the initiator of polymerization. pH, volume of rinsing and eluent solvent and amount of sorbent influence on response were investigated using factorial experimental design, while optimum point was achieved and set as 250mg sorbent, pH 7.0, 1.5mL washing solvent and 2mL eluent by analysis of results according to design expert (DX) software. At above specified conditions, CEL in human plasma with complicated matrices with acceptable high recoveries (96%) and RSD% lower than 10% was quantified and estimated. The proposed MISPE-HPLC-UV method has linear responses among peak area and concentrations of CEL in the range of 0.2-2000μgL(-1), with regression coefficient of 0.98. The limit of detection (LOD) and quantification (LOQ) based on three and ten times of the noise of HPLC peaks correspond to blank solution were 0.08 and 0.18μgL(-1), respectively.
authors.affiliation: Chemistry Department, Yasouj University, Yasouj 75918-74831, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Arabi
authors.affiliation: Chemistry Department, Yasouj University, Yasouj 75918-74831, Iran. Electronic address: m_ghaedi@mail.yu.ac.ir.
authors.forename: Mehrorang
authors.initials: M
authors.lastname: Ghaedi
authors.affiliation: Department of Chemistry, Kerman Branch, Islamic Azad University, Kerman, Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Ostovan
authors.affiliation: Department of Chemistry, College of Science, Shiraz University, Shiraz 71454, Iran.
authors.forename: Javad
authors.initials: J
authors.lastname: Tashkhourian
authors.affiliation: Department of Chemistry, Kerman Branch, Islamic Azad University, Kerman, Iran.
authors.forename: Hamideh
authors.initials: H
authors.lastname: Asadallahzadeh
mesh_terms: D000068579:Celecoxib / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D054802:Molecular Imprinting; D053758:Nanoparticles; D011108:Polymers; D012984:Software; D052616:Solid Phase Extraction; D014465:Ultrasonics
chemical_list: D011108:Polymers; D000068579:Celecoxib
keywords: Celecoxib; Design expert; HPLC–UV; Human plasma; Molecular imprinted nanoparticles; Solid phase extraction