title: miR-125b inhibited epithelial-mesenchymal transition of triple-negative breast cancer by targeting MAP2K7.
abstract: MicroRNAs (miRNAs) play important roles in diverse biological processes and are emerging as key regulators of tumorigenesis and tumor progression. Among the differentially expressed miRNAs in breast cancer, miR-125b was revealed to be deregulated and associated with poor prognosis and chemoresistance in triple-negative breast cancer (TNBC), but the mechanism is still unknown. In our study, we showed downregulated expression of miR-125b in TNBC tissues and decreased migration and invasion in miR-125b-expressing Hs578T cells. MAP2K7 was then detected to be a novel target of miR-125b, and downregulation of MAP2K7 by miR-125b was similar to transient knockdown of MAP2K7 which hindered epithelial-mesenchymal transition (EMT) of Hs578T cells. Upregulation of MAP2K7 in miR-125b-overexpressing Hs578T cells partly rescued the migration and invasion suppression of miR-125b. Furthermore, MAP2K7 was overexpressed in TNBC samples compared with normal tissues and negatively correlated with miR-125b expression. In light of these findings, miR-125b emerged as a tumor suppressor in TNBC by targeting MAP2K7 to inhibit EMT.
authors.affiliation: Department of Clinical Laboratory, Affiliated Hospital of Hangzhou Normal University, Hangzhou, Zhejiang Province, People's Republic of China.
authors.forename: Liquan
authors.initials: L
authors.lastname: Hong
authors.affiliation: Department of Clinical Laboratory, Affiliated Hospital of Hangzhou Normal University, Hangzhou, Zhejiang Province, People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Pan
authors.affiliation: Zhejiang Provincial Tumor Hospital, Hangzhou, Zhejiang Province, People's Republic of China.
authors.forename: Huifen
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Department of Clinical Laboratory, Affiliated Hospital of Hangzhou Normal University, Hangzhou, Zhejiang Province, People's Republic of China.
authors.forename: Lahong
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratory, Affiliated Hospital of Hangzhou Normal University, Hangzhou, Zhejiang Province, People's Republic of China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Clinical Laboratory, Affiliated Hospital of Hangzhou Normal University, Hangzhou, Zhejiang Province, People's Republic of China.
authors.forename: Chengsong
authors.initials: C
authors.lastname: Cai
authors.affiliation: Zhejiang Provincial Children's Hospital, Hangzhou, Zhejiang Province, People's Republic of China.
authors.forename: Chunzhen
authors.initials: C
authors.lastname: Hua
authors.affiliation: Department of Clinical Laboratory, Affiliated Hospital of Hangzhou Normal University, Hangzhou, Zhejiang Province, People's Republic of China.
authors.forename: Xian
authors.initials: X
authors.lastname: Luo
authors.affiliation: Technology Department, Hangzhou Joingenome Diagnostics, Hangzhou, Zhejiang Province, People's Republic of China.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Clinical Laboratory, Affiliated Hospital of Hangzhou Normal University, Hangzhou, Zhejiang Province, People's Republic of China.
authors.forename: Zhaojun
authors.initials: Z
authors.lastname: Chen
keywords: EMT; Hs578T; MAP2K7; TNBC; miR-125b