title: Phospholipase A2 as a point of care alternative to serum amylase and pancreatic lipase.
abstract: Acute pancreatitis is a relatively common and potentially fatal condition, but the presenting symptoms are non-specific and diagnosis relies largely on the measurement of amylase activity by the hospital clinical laboratory. In this work we develop a point of care test for pancreatitis measuring concentration of secretory phospholipase A2 group IB (sPLA2-IB). Novel antibodies for sPLA2-IB were raised and used to design an ELISA and a lateral flow device (LFD) for the point of care measurement of sPLA2-IB concentration, which was compared to pancreatic amylase activity, lipase activity, and sPLA2-IB activity in 153 serum samples. 98 of these samples were obtained from the pathology unit of a major hospital and classified retrospectively according to presence or absence of pancreatitis, and the remaining 55 were obtained from commercial sources to serve as high lipase (n = 20), CA19-9 positive (n = 15), and healthy (n = 20) controls. sPLA2-IB concentration correlated well with the serum activity of both amylase and lipase, and performed at least as well as either markers in the differentiation of pancreatitis from controls.
authors.affiliation: Department of Materials, Department of Bioengineering and Institute for Biomedical Engineering, Imperial College London, London SW7 2AZ, UK. m.stevens@imperial.ac.uk.
authors.forename: Nathan J
authors.initials: NJ
authors.lastname: Liu
authors.affiliation: Department of Materials, Department of Bioengineering and Institute for Biomedical Engineering, Imperial College London, London SW7 2AZ, UK. m.stevens@imperial.ac.uk.
authors.forename: Robert
authors.initials: R
authors.lastname: Chapman
authors.affiliation: Department of Materials, Department of Bioengineering and Institute for Biomedical Engineering, Imperial College London, London SW7 2AZ, UK. m.stevens@imperial.ac.uk.
authors.forename: Yiyang
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Mologic Ltd, Bedford Technology Park, Thurleigh, Bedfordshire MK44 2YP, UK.
authors.forename: Andrew
authors.initials: A
authors.lastname: Bentham
authors.affiliation: Mologic Ltd, Bedford Technology Park, Thurleigh, Bedfordshire MK44 2YP, UK.
authors.forename: Matthew
authors.initials: M
authors.lastname: Tyreman
authors.affiliation: Hepatology and Gastroenterology Section, Department of Medicine, St Mary's Hospital Campus, Imperial College London, London, W2 1NY, UK.
authors.forename: Natalie
authors.initials: N
authors.lastname: Philips
authors.affiliation: Hepatology and Gastroenterology Section, Department of Medicine, St Mary's Hospital Campus, Imperial College London, London, W2 1NY, UK.
authors.forename: Shahid A
authors.initials: SA
authors.lastname: Khan
authors.affiliation: Department of Materials, Department of Bioengineering and Institute for Biomedical Engineering, Imperial College London, London SW7 2AZ, UK. m.stevens@imperial.ac.uk.
authors.forename: Molly M
authors.initials: MM
authors.lastname: Stevens
mesh_terms: D000208:Acute Disease; D000681:Amylases / Q000097:blood*; D006801:Humans; D008049:Lipase / Q000097:blood*; D010195:Pancreatitis / Q000175:diagnosis*; D054467:Phospholipases A2 / Q000097:blood*; D019095:Point-of-Care Systems
chemical_list: D008049:Lipase; D054467:Phospholipases A2; D000681:Amylases