title: The Shank3 Interaction Partner ProSAPiP1 Regulates Postsynaptic SPAR Levels and the Maturation of Dendritic Spines in Hippocampal Neurons.
abstract: The postsynaptic density or PSD is a submembranous compartment containing a wide array of proteins that contribute to both morphology and function of excitatory glutamatergic synapses. In this study, we have analyzed functional aspects of the Fezzin ProSAP-interacting protein 1 (ProSAPiP1), an interaction partner of the well-known PSD proteins Shank3 and SPAR. Using lentiviral-mediated overexpression and knockdown of ProSAPiP1, we found that this protein is dispensable for the formation of both pre- and postsynaptic specializations per se. We further show that ProSAPiP1 regulates SPAR levels at the PSD and the maturation of dendritic spines. In line with previous findings on the ProSAPiP1 homolog PSD-Zip70, we conclude that Fezzins essentially contribute to the maturation of excitatory spine synapses.
authors.affiliation: Institute for Anatomy and Cell Biology, Ulm UniversityUlm, Germany; International Graduate School in Molecular Medicine, Ulm UniversityUlm, Germany.
authors.forename: Dominik
authors.initials: D
authors.lastname: Reim
authors.affiliation: Institute for Anatomy and Cell Biology, Ulm UniversityUlm, Germany; International Graduate School in Molecular Medicine, Ulm UniversityUlm, Germany.
authors.forename: Tobias M
authors.initials: TM
authors.lastname: Weis
authors.affiliation: Institute for Anatomy and Cell Biology, Ulm UniversityUlm, Germany; International Graduate School in Molecular Medicine, Ulm UniversityUlm, Germany.
authors.forename: Sonja
authors.initials: S
authors.lastname: Halbedl
authors.affiliation: Institute for Anatomy and Cell Biology, Ulm UniversityUlm, Germany; International Graduate School in Molecular Medicine, Ulm UniversityUlm, Germany.
authors.forename: Jan Philipp
authors.initials: JP
authors.lastname: Delling
authors.affiliation: Institute for Anatomy and Cell Biology, Ulm UniversityUlm, Germany; WG Molecular Analysis of Synaptopathies, Department of Neurology, Neurocenter of Ulm UniversityUlm, Germany.
authors.forename: Andreas M
authors.initials: AM
authors.lastname: Grabrucker
authors.affiliation: Institute for Anatomy and Cell Biology, Ulm University Ulm, Germany.
authors.forename: Tobias M
authors.initials: TM
authors.lastname: Boeckers
authors.affiliation: Institute for Anatomy and Cell Biology, Ulm UniversityUlm, Germany; Department of Neurology, Ulm UniversityUlm, Germany.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Schmeisser
keywords: LAPSER1; LZTS2; LZTS3; PSD-Zip70; ProSAPiP1; SPAR; Shank3; synapse