title: Effects of organic acids on the photosynthetic and antioxidant properties and accumulations of heavy metals of Melilotus officinalis grown in Cu tailing.
abstract: The effect of citric acid (CA), acetic acid (Ac), and ethylene diamine tetraacetic acid (EDTA) on the photosynthetic and antioxidant properties and the accumulation of some heavy metals (HMs) of Melilotus officinalis seedling growing in Cu mine tailings for 25 days were studied. Results showed that the formation of photosynthesizing cells of M. officinalis was inhibited by EDTA at 2 mmol/kg. Photosynthetic pigment contents under EDTA of 2 mmol/kg were reduced by 26, 40, and 19 %, respectively, compared to the control. The proline contents in aboveground and underground parts increased as the level of EDTA was enhanced. CA and Ac enhanced the activities of superoxide dismutase (SOD) and peroxidase (POD) in the aboveground parts and EDTA inhibited the activity of POD in the underground parts. The addition of CA promoted significantly the growth of M. officinalis, while the biomass decreased significantly under 2 mmol/kg EDTA. Cu contents in the aboveground parts treated with 0.5 and 2.0 mmol/kg EDTA reached 175.50 and 265.17 μg/g dry weight, respectively. Ac and EDTA treatments promoted Cd to translocate from root to aboveground parts. The result indicated that M. officinalis was a tolerant species of Cu tailing and can be used to remediate Cu contaminated environment, and rationally utilization of organic acids, especially EDTA, in the phytoremediation can improve the growth and metals accumulation of M. officinalis.
authors.affiliation: Experimental Teaching Center of Ecological Environment of Jiangxi Province, Department of Landscape Architecture, Jiangxi University of Finance and Economics, Nanchang, 330032, China. hyl1957@163.com.|Nanchang Institute of Science and Technology, Nanchang, 330108, China. hyl1957@163.com.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Han
authors.affiliation: Experimental Teaching Center of Ecological Environment of Jiangxi Province, Department of Landscape Architecture, Jiangxi University of Finance and Economics, Nanchang, 330032, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Wu
authors.affiliation: College of Life Science and Technology, Jinan University, Guangzhou, 510632, China.
authors.forename: Jiguang
authors.initials: J
authors.lastname: Gu
authors.affiliation: Experimental Teaching Center of Ecological Environment of Jiangxi Province, Department of Landscape Architecture, Jiangxi University of Finance and Economics, Nanchang, 330032, China.
authors.forename: Jiuzhou
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Institute of Botany, Jiangsu Province and Chinese Academy of Science, Nanjing Sun Yat-Sen Botanical Garden, Nanjing, 210014, China.
authors.forename: Suzhen
authors.initials: S
authors.lastname: Huang
authors.affiliation: Institute of Botany, Jiangsu Province and Chinese Academy of Science, Nanjing Sun Yat-Sen Botanical Garden, Nanjing, 210014, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Yuan
authors.affiliation: Nanchang Institute of Science and Technology, Nanchang, 330108, China.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Fu
mesh_terms: D019342:Acetic Acid / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D019343:Citric Acid / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology*; D029903:Melilotus / Q000187:drug effects / Q000254:growth & development; D009195:Peroxidase / Q000378:metabolism; D010788:Photosynthesis / Q000187:drug effects*; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D019343:Citric Acid; D004492:Edetic Acid; D009195:Peroxidase; D013482:Superoxide Dismutase; D019342:Acetic Acid
keywords: Copper (Cu); Melilotus officinalis; Mine tailing; Organic acids; Physiological response; Phytoremediation