title: ELS1, a novel MATE transporter related to leaf senescence and iron homeostasis in Arabidopsis thaliana.
abstract: The multidrug and toxic compound extrusion (MATE) transporters mediate the coupled exchange of organic substrates and monovalent cations have been recently implicated in various plant biological activities. In this work, we isolated a dominant mutant from an Arabidopsis activation-tagging mutant pool. This mutant exhibits pleiotropic phenotype including early flowering, dwarf and bushy architecture, minified lateral organs and early leaf senescence, and is therefore designated early leaf senescence 1-Dominaint (els1-D). Genotyping assays showed that els1-D is a gain-of-function mutant of a novel MATE transporter gene, ELS1, which encodes a close homolog of the previously reported ADP1, BCD1 and DTX50. Further investigations revealed that the overexpression of ELS1 reduces iron content in els1-D, and the accelerated senescence of the detached els1-D leaves can be recovered by exogenous iron supply. In addition, we also found that ELS1 is an iron responsive gene. Based on these findings, we proposed that ELS1 is related to leaf senescence and iron homeostasis in Arabidopsis.
authors.affiliation: Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou 730030, China. Electronic address: wzy72609@163.com.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou 730030, China. Electronic address: qiancz11@lzu.edu.cn.
authors.forename: Chongzhen
authors.initials: C
authors.lastname: Qian
authors.affiliation: Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou 730030, China. Electronic address: guoxch13@lzu.edu.cn.
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Guo
authors.affiliation: Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou 730030, China. Electronic address: iuel14@lzu.edu.cn.
authors.forename: Erlong
authors.initials: E
authors.lastname: Liu
authors.affiliation: Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou 730030, China. Electronic address: maokl14@lzu.edu.cn.
authors.forename: Kaili
authors.initials: K
authors.lastname: Mao
authors.affiliation: Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou 730030, China. Electronic address: mucj@lzu.edu.cn.
authors.forename: Changjun
authors.initials: C
authors.lastname: Mu
authors.affiliation: Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou 730030, China. Electronic address: 63710156@qq.com.
authors.forename: Ni
authors.initials: N
authors.lastname: Chen
authors.affiliation: Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai 200444, China. Electronic address: wzhang1216@yahoo.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou 730030, China. Electronic address: hengliu@lzu.edu.cn.
authors.forename: Heng
authors.initials: H
authors.lastname: Liu
mesh_terms: D000375:Aging / Q000502:physiology; D017360:Arabidopsis / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D006706:Homeostasis / Q000502:physiology; D007501:Iron / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D027701:Organic Cation Transport Proteins / Q000235:genetics; D018515:Plant Leaves / Q000235:genetics / Q000502:physiology*; D030821:Plants, Genetically Modified
chemical_list: D029681:Arabidopsis Proteins; C000588907:At4g29140 protein, Arabidopsis; C000619255:ELS1 MATE transporter, Arabidopsis; D026901:Membrane Transport Proteins; D027701:Organic Cation Transport Proteins; D007501:Iron
keywords: ELS1; Iron homeostasis; Leaf senescence; MATE transporter