title: Effects of Fluoride on Expression of P450, CREM and ACT Proteins in Rat Testes.
abstract: Fluoride (F) is an essential trace element that humans and animals ingest from water, air, and fluoride-containing products; however, excessive fluoride absorption can damage a variety of organs and tissues, including the male reproductive system. Our previous studies found that fluoride exposure lowered sperm quality and interfered with spermatogenesis; however, the exact mechanism remained unclear. Proteins cytochrome P450 (P450), cAMP-responsive element modulator (CREM), and activator of CREM in testis (ACT) play the key roles in spermatogenesis and sperm motility. To investigate whether fluoride affects the expression of P450, CREM, and ACT, we used immunohistochemical techniques to determine expression levels of these proteins in testes of rats administered 100 mg NaF/L for 2 weeks via drinking water. The results showed that P450 expression was decreased while CREM and ACT expression was increased in the fluoride group, compared to the control. These data suggest that fluoride can impair male reproduction by affecting expression of P450, CREM, and ACT in the testes.
authors.affiliation: Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, 030801, China. Jianhaiz@163.com.
authors.forename: Jianhai
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, 030801, China.
authors.forename: Yuchen
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, 030801, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Liang
authors.affiliation: Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, 030801, China.
authors.forename: Mingli
authors.initials: M
authors.lastname: Qie
authors.affiliation: Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, 030801, China.
authors.forename: Ruiyan
authors.initials: R
authors.lastname: Niu
authors.affiliation: Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, 030801, China.
authors.forename: Zilong
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, 030801, China.
authors.forename: Jinming
authors.initials: J
authors.lastname: Wang
authors.affiliation: Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, 030801, China. wangjd53@outlook.com.
authors.forename: Jundong
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D051786:Cyclic AMP Response Element Modulator / Q000096:biosynthesis*; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D005459:Fluorides / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013081:Sperm Motility / Q000187:drug effects; D013091:Spermatogenesis / Q000187:drug effects; D013737:Testis / Q000378:metabolism*; D014157:Transcription Factors / Q000096:biosynthesis*
chemical_list: C497412:Crem protein, rat; D014157:Transcription Factors; C117930:activator of CREM in testis (ACT); D051786:Cyclic AMP Response Element Modulator; D003577:Cytochrome P-450 Enzyme System; D005459:Fluorides
keywords: ACT; CREM; Fluoride; P450; Testes