title: Measuring ECS Interaction with Biomembranes.
abstract: Understanding the correct interaction among the different components of the endocannabinoid system (ECS) is fundamental for a proper assessment of the function of endocannabinoids (eCBs) as signaling molecules. The knowledge of how membrane environment is able to modulate intracellular trafficking of eCBs and their interacting proteins holds a huge potential in unraveling new mechanisms of ECS modulation.Here, fluorescence resonance energy transfer (FRET) technique is applied to measure the binding affinity of ECS proteins to model membranes (i.e., large unilamellar vesicles, LUVs). In particular, we describe in details the paradigmatic example of the interaction of recombinant rat FAAH-ΔTM with LUVs constituted by 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC).
authors.affiliation: Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy.
authors.forename: Clotilde B
authors.initials: CB
authors.lastname: Angelucci
authors.affiliation: Faculty of Bioscience and Technology for Food Agriculture and Environment, University of Teramo, Teramo, Italy.
authors.forename: Annalaura
authors.initials: A
authors.lastname: Sabatucci
authors.affiliation: Faculty of Bioscience and Technology for Food Agriculture and Environment, University of Teramo, Teramo, Italy. edainese@unite.it.
authors.forename: Enrico
authors.initials: E
authors.lastname: Dainese
mesh_terms: D000818:Animals; D001681:Biological Assay; D001692:Biological Transport; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D063388:Endocannabinoids / Q000737:chemistry / Q000378:metabolism*; D031541:Fluorescence Resonance Energy Transfer; D007425:Intracellular Membranes / Q000737:chemistry / Q000378:metabolism; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D011485:Protein Binding; D051381:Rats; D015398:Signal Transduction; D013696:Temperature
chemical_list: D063388:Endocannabinoids; D008051:Lipid Bilayers; D008081:Liposomes
keywords: Binding isotherms; Biological membranes; FRET; Large unilamellar vesicles; Membrane affinity; Membrane proteins