title: A pyrene-benzthiazolium conjugate portraying aggregation induced emission, a ratiometric detection and live cell visualization of HSO3(.).
abstract: The present study deals with the photophysical property of a pyrene-benzthiazolium conjugate R1, as a strong intramolecular charge transfer (ICT) probe exhibiting long wavelength emission in the red region. Unlike traditional planar polyaromatic hydrocarbons whose aggregation generally quenches the light emission, the pyrene based R1 was found to display aggregation-induced emission (AIE) property along with simultaneous increase in its quantum yield upon increasing the water content of the medium. The R1 exhibits high specificity towards HSO3(-)/SO3(2-) by interrupting its own ICT producing there upon a large ratiometric blue shift of ∼220 nm in its emission spectrum. The lowest detection limit for the above measurement was found to be 8.90 × 10(-8) M. The fluorescent detection of HSO3(-) was also demonstrated excellently by test paper strip and silica coated TLC plate incorporating R1. The live cell imaging of HSO3(─) through R1 in HeLa cells was studied using fluorescence microscopic studies. The particle size and morphological features of R1 and R1-HSO3(-) aggregates in aqueous solution were characterized by DLS along with SEM analysis.
authors.affiliation: Department of Chemistry (Centre of Advanced Study), Faculty of Science, Banaras Hindu University, Varanasi, Uttar Pradesh 221005, India.
authors.forename: Uzra
authors.initials: U
authors.lastname: Diwan
authors.affiliation: Department of Chemistry (Centre of Advanced Study), Faculty of Science, Banaras Hindu University, Varanasi, Uttar Pradesh 221005, India.
authors.forename: Virendra
authors.initials: V
authors.lastname: Kumar
authors.affiliation: Photosciences and Photonics, Chemical Sciences and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Thiruvananthapuram 695019, India.
authors.forename: Rakesh K
authors.initials: RK
authors.lastname: Mishra
authors.affiliation: Department of Zoology (Centre of Advanced Study), Faculty of Science, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Nishant Kumar
authors.initials: NK
authors.lastname: Rana
authors.affiliation: Department of Zoology (Centre of Advanced Study), Faculty of Science, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Biplob
authors.initials: B
authors.lastname: Koch
authors.affiliation: Department of Zoology (Centre of Advanced Study), Faculty of Science, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Manish Kumar
authors.initials: MK
authors.lastname: Singh
authors.affiliation: Department of Chemistry (Centre of Advanced Study), Faculty of Science, Banaras Hindu University, Varanasi, Uttar Pradesh 221005, India. Electronic address: drkaushalbhu@yahoo.co.in.
authors.forename: K K
authors.initials: KK
authors.lastname: Upadhyay
mesh_terms: D002470:Cell Survival; D006367:HeLa Cells; D006801:Humans; D008958:Models, Molecular; D008968:Molecular Conformation; D057054:Molecular Imaging / Q000379:methods*; D011721:Pyrenes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013464:Sulfuric Acids / Q000737:chemistry / Q000378:metabolism*; D013844:Thiazoles / Q000737:chemistry*
chemical_list: D011721:Pyrenes; D013464:Sulfuric Acids; D013844:Thiazoles; C030984:pyrene; C033158:sulfuric acid
keywords: Aggregation-induced emission; Bio-imaging; ICT; Optical sensors; Pyrene