title: The CebE/MsiK Transporter is a Doorway to the Cello-oligosaccharide-mediated Induction of Streptomyces scabies Pathogenicity.
abstract: Streptomyces scabies is an economically important plant pathogen well-known for damaging root and tuber crops by causing scab lesions. Thaxtomin A is the main causative agent responsible for the pathogenicity of S. scabies and cello-oligosaccharides are environmental triggers that induce the production of this phytotoxin. How cello-oligosaccharides are sensed or transported in order to induce the virulent behavior of S. scabies? Here we report that the cellobiose and cellotriose binding protein CebE, and MsiK, the ATPase providing energy for carbohydrates transport, are the protagonists of the cello-oligosaccharide mediated induction of thaxtomin production in S. scabies. Our work provides the first example where the transport and not the sensing of major constituents of the plant host is the central mechanism associated with virulence of the pathogen. Our results allow to draw a complete pathway from signal transport to phytotoxin production where each step of the cascade is controlled by CebR, the cellulose utilization regulator. We propose the high affinity of CebE to cellotriose as possible adaptation of S. scabies to colonize expanding plant tissue. Our work further highlights how genes associated with primary metabolism in nonpathogenic Streptomyces species have been recruited as basic elements of virulence in plant pathogenic species.
authors.affiliation: Centre for Protein Engineering, Integrative Biological Sciences (InBioS) Research Unit, University of Liège, Institut de Chimie B6a, B-4000, Liège, Belgium.
authors.forename: Samuel
authors.initials: S
authors.lastname: Jourdan
authors.affiliation: Department of Biology, California State University Bakersfield, Bakersfield, CA 93311-1022, USA.|Department of Plant Pathology, University of Florida, Gainesville, FL 32611-0180, USA.
authors.forename: Isolde Maria
authors.initials: IM
authors.lastname: Francis
authors.affiliation: Department of Plant Pathology, University of Florida, Gainesville, FL 32611-0180, USA.
authors.forename: Min Jung
authors.initials: MJ
authors.lastname: Kim
authors.affiliation: Department of Biology, California State University Bakersfield, Bakersfield, CA 93311-1022, USA.
authors.forename: Joren Jeico C
authors.initials: JJ
authors.lastname: Salazar
authors.affiliation: Laboratory for Protein Biochemistry and Biomolecular Engineering, Department of Biochemistry and Microbiology, Ghent University, B-9000, Ghent, Belgium.
authors.forename: Sören
authors.initials: S
authors.lastname: Planckaert
authors.affiliation: Centre for Protein Engineering, Integrative Biological Sciences (InBioS) Research Unit, University of Liège, Institut de Chimie B6a, B-4000, Liège, Belgium.
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Frère
authors.affiliation: Centre for Protein Engineering, Integrative Biological Sciences (InBioS) Research Unit, University of Liège, Institut de Chimie B6a, B-4000, Liège, Belgium.
authors.forename: André
authors.initials: A
authors.lastname: Matagne
authors.affiliation: Centre for Protein Engineering, Integrative Biological Sciences (InBioS) Research Unit, University of Liège, Institut de Chimie B6a, B-4000, Liège, Belgium.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Kerff
authors.affiliation: Laboratory for Protein Biochemistry and Biomolecular Engineering, Department of Biochemistry and Microbiology, Ghent University, B-9000, Ghent, Belgium.
authors.forename: Bart
authors.initials: B
authors.lastname: Devreese
authors.affiliation: Department of Plant Pathology, University of Florida, Gainesville, FL 32611-0180, USA.
authors.forename: Rosemary
authors.initials: R
authors.lastname: Loria
authors.affiliation: Centre for Protein Engineering, Integrative Biological Sciences (InBioS) Research Unit, University of Liège, Institut de Chimie B6a, B-4000, Liège, Belgium.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Rigali
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000378:metabolism*; D000251:Adenosine Triphosphatases / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism*; D002475:Cellobiose / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D007211:Indoles / Q000378:metabolism; D009844:Oligosaccharides / Q000378:metabolism*; D010802:Phylogeny; D010879:Piperazines / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology; D010940:Plant Proteins / Q000378:metabolism; D021381:Protein Transport; D015398:Signal Transduction; D013302:Streptomyces / Q000378:metabolism / Q000472:pathogenicity*
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D007211:Indoles; D009844:Oligosaccharides; D010879:Piperazines; D010940:Plant Proteins; C406691:thaxtomine A; D002475:Cellobiose; D000251:Adenosine Triphosphatases; C096958:MsiK protein, Streptomyces