title: Mechanoresponsiveness of human umbilical cord mesenchymal stem cells in in vitro chondrogenesis-A comparative study with growth factor induction.
abstract: Fetal-derived mesenchymal stem cells especially human umbilical cord matrix mesenchymal stem cells (hUCMSCs), with their ease of availability, pluripotency, and high expansion potential have emerged as an alternative solution for stem cell based cartilage therapies. An attempt to elucidate the effect of dynamic mechanical compression in modulating the chondrogenic differentiation of hUCMSCs is done in this study to add on to the knowledge of optimizing chondrogenic signals necessary for the effective differentiation of these stem cells and subsequent integration to the surrounding tissues. hUCMSCs were seeded in porous poly (vinyl alcohol)-poly (caprolactone) (PVA-PCL) scaffolds and cultured in chondrogenic medium with/without TGF-β3 and were subjected to a dynamic compression of 10% strain, 1 Hz for 1/4 h for 7 days. The results on various analysis shows that the extent of dynamic compression is an important factor affecting cell viability. Mechanical stimulation in the form of dynamic compression stimulates expression of chondrogenic genes even in the absence of chondrogenic growth factors and also augments growth factor induced chondrogenic potential of hUCMSC. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2554-2566, 2016.
authors.affiliation: Division of Tissue Engineering and Regeneration Technologies, BMT Wing, Sree Chithra Tirunal Institute for Medical Sciences and Technology, Trivandrum, 695012, India.
authors.forename: N S
authors.initials: NS
authors.lastname: Remya
authors.affiliation: Division of Tissue Engineering and Regeneration Technologies, BMT Wing, Sree Chithra Tirunal Institute for Medical Sciences and Technology, Trivandrum, 695012, India.
authors.forename: Prabha D
authors.initials: PD
authors.lastname: Nair
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects; D020219:Chondrogenesis / Q000187:drug effects*; D004867:Equipment Design; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D011091:Polyesters / Q000737:chemistry; D011142:Polyvinyl Alcohol / Q000031:analogs & derivatives; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D053782:Transforming Growth Factor beta3 / Q000494:pharmacology*; D014470:Umbilical Cord / Q000166:cytology
chemical_list: D011091:Polyesters; D053782:Transforming Growth Factor beta3; C016240:polycaprolactone; D011142:Polyvinyl Alcohol
keywords: chondrogenic differentiation; dynamic compression; mechano transduction; mesenchymal stem cells; three dimensional scaffolds