title: Iridium Complexes as a Roadblock for DNA Polymerase during Amplification.
abstract: Iridium-based metal complexes containing polypyridyl-pyrazine ligands show properties of DNA intercalation. They serve as roadblocks to DNA polymerase activity, thereby inhibiting the polymerization process. Upon the addition of increasing concentrations of these iridium complexes, a rapid polymerase chain reaction (PCR)-based assay reveals the selective inhibition of the DNA polymerization process. This label-free approach to study the inhibition of fundamental cellular processes via physical roadblock can offer an alternative route toward cancer therapy.
authors.affiliation: Department of Chemistry, Indian Institute of Science Education and Research Bhopal, Bhopal By Pass Road, Bhauri, 462066, Bhopal, India.
authors.forename: Falguni
authors.initials: F
authors.lastname: Chandra
authors.affiliation: Department of Chemistry, Indian Institute of Science Education and Research Bhopal, Bhopal By Pass Road, Bhauri, 462066, Bhopal, India.
authors.forename: Prashant
authors.initials: P
authors.lastname: Kumar
authors.affiliation: School of Basic Sciences, Indian Institute of Technology Bhubaneswar, 751007, Bhubaneswar, India.
authors.forename: Suman Kumar
authors.initials: SK
authors.lastname: Tripathi
authors.affiliation: School of Basic Sciences, Indian Institute of Technology Bhubaneswar, 751007, Bhubaneswar, India. srikanta@iitbbs.ac.in.
authors.forename: Srikanta
authors.initials: S
authors.lastname: Patra
authors.affiliation: Department of Chemistry, Indian Institute of Science Education and Research Bhopal, Bhopal By Pass Road, Bhauri, 462066, Bhopal, India. akoner@iiserb.ac.in.
authors.forename: Apurba L
authors.identifier: 0000-0002-8891-416X
authors.initials: AL
authors.lastname: Koner
mesh_terms: D056831:Coordination Complexes / Q000737:chemistry*; D004247:DNA / Q000737:chemistry; D007364:Intercalating Agents / Q000737:chemistry*; D007495:Iridium / Q000737:chemistry*; D008024:Ligands; D019384:Nucleic Acid Synthesis Inhibitors / Q000737:chemistry*; D010957:Plasmids; D016133:Polymerase Chain Reaction; D058105:Polymerization; D011719:Pyrazines / Q000737:chemistry; D011725:Pyridines / Q000737:chemistry
chemical_list: D056831:Coordination Complexes; D007364:Intercalating Agents; D008024:Ligands; D019384:Nucleic Acid Synthesis Inhibitors; D011719:Pyrazines; D011725:Pyridines; D007495:Iridium; D004247:DNA
keywords: DNA intercalation; DNA polymerase; antitumor agents; cancer; iridium complexes