title: Isolation of Eosinophils from the Lamina Propria of the Murine Small Intestine.
abstract: Only recently has it become apparent that eosinophils play a crucial role in mucosal immune homeostasis. Although eosinophils are the main cellular component of the lamina propria of the gastrointestinal tract, they have often been overlooked because they express numerous markers, which are normally used to characterize macrophages and/or dendritic cells. To study their function in mucosal immunity, it is important to isolate them with high purity and viability. Here, we describe a protocol to purify eosinophils from the lamina propria of the murine small intestine. The method involves preparation of the small intestine, removal of epithelial cells and digestion of the lamina propria to release eosinophils. A protocol to sort eosinophils is included.
authors.affiliation: German Rheumatism Research Center (DRFZ), A Leibniz Institute, Chariteplatz 1, 10117, Berlin, Germany. berek@drfz.de.
authors.forename: Claudia
authors.initials: C
authors.lastname: Berek
authors.affiliation: German Rheumatism Research Center (DRFZ), A Leibniz Institute, Chariteplatz 1, 10117, Berlin, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Beller
authors.affiliation: German Rheumatism Research Center (DRFZ), A Leibniz Institute, Chariteplatz 1, 10117, Berlin, Germany.|Immune Regulation and Cancer, Max-Delbrück-Center for Molecular Medicine, Berlin, Germany.
authors.forename: Van Trung
authors.initials: VT
authors.lastname: Chu
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D004804:Eosinophils / Q000166:cytology*; D005434:Flow Cytometry; D005753:Gastric Mucosa / Q000166:cytology; D007421:Intestine, Small / Q000166:cytology*; D051379:Mice; D009092:Mucous Membrane / Q000166:cytology
keywords: Digestion; Eosinophils; Epithelial cells; Lamina propria; Peyer’s patches; Small intestine