title: The functional response of bioactive titania-modified three-dimensional Ti-6Al-4V mesh structure toward providing a favorable pathway for intercellular communication and osteoincorporation.
abstract: The objective of the study is to fundamentally elucidate the biological response of 3D printed mesh structures subjected to plasma electrolytic oxidation process through the study of osteoblast functions. The cellular activity of plasma electrolytic-oxidized mesh structure was explored in terms of cell-to-cell communication involving proliferation, synthesis of extracellular and intracellular proteins, and mineralization. Upon plasma electrolytic oxidation of the mesh structure, a thin layer of bioactive titania with pore size 1-3 µm was nucleated on the surface. The combination of microporous bioactive titania and interconnected porous architecture provided the desired pathway for supply of nutrients and oxygen to cells and tissue and a favorable osteogenic microenvironment for tissue on-growth and in-growth, in relation to the unmodified mesh structure. The formation of a confluent layer as envisaged via electron microscopy and quantitative assessment of the expression level of proteins (actin, vinculin, and fibronectin) point toward the determining role of surface-modified mesh structure in modulating osteoblasts functions. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2488-2501, 2016.
authors.affiliation: Biomaterials and Biomedical Engineering Research Laboratory, Department of Metallurgical, Materials and Biomedical Engineering, The University of Texas at El Paso, 500 W. University Avenue, El Paso, Texas, 79968.
authors.forename: K C
authors.initials: KC
authors.lastname: Nune
authors.affiliation: Biomaterials and Biomedical Engineering Research Laboratory, Department of Metallurgical, Materials and Biomedical Engineering, The University of Texas at El Paso, 500 W. University Avenue, El Paso, Texas, 79968.
authors.forename: R D K
authors.initials: RD
authors.lastname: Misra
authors.affiliation: Shenyang National Laboratory for Materials Science, Institute of Metal Research, Chinese Academy of Sciences, 72 Wenhua Road, Shenyang, 110016, China.
authors.forename: S J
authors.initials: SJ
authors.lastname: Li
authors.affiliation: Shenyang National Laboratory for Materials Science, Institute of Metal Research, Chinese Academy of Sciences, 72 Wenhua Road, Shenyang, 110016, China.
authors.forename: Y L
authors.initials: YL
authors.lastname: Hao
authors.affiliation: Laboratory for Corrosion and Protection, Institute of Metal Research, Chinese Academy of Sciences, Shenyang, 110016, China.
authors.forename: W
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000497:Alloys / Q000737:chemistry / Q000378:metabolism*; D000535:Aluminum / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism*; D002450:Cell Communication; D002460:Cell Line; D049109:Cell Proliferation; D005109:Extracellular Matrix / Q000378:metabolism; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D010084:Oxidation-Reduction; D016062:Porosity; D014025:Titanium / Q000737:chemistry / Q000378:metabolism*; D014639:Vanadium / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000497:Alloys; D001672:Biocompatible Materials; D014639:Vanadium; C009495:titanium dioxide; D000535:Aluminum; D014025:Titanium
keywords: Ti6Al4V; mesh structure; micro-arc oxidation; osteoblasts