title: Human amniotic fluid stem cells labeled with up-conversion nanoparticles for imaging-monitored repairing of acute lung injury.
abstract: Human amniotic fluid stem (hAFS) cells have generated a great deal of excitement in cell-based therapies and regenerative medicine. Here, we examined the effect of hAFS cells labeled with dual-polymer-coated UCNP-PEG-PEI nanoparticles in a murine model of acute lung injury (ALI). We observed hAFS cells migration to the lung using highly sensitive in vivo upconversion luminescence (UCL) imaging. We demonstrated that hAFS cells remained viable and retained their ability to differentiate even after UCNP-PEG-PEI labeling. More importantly, hAFS cells displayed remarkable positive effects on ALI-damaged lung tissue repair compared with mouse bone marrow mesenchymal stem cells (mBMSCs), which include recovery of the integrity of alveolar-capillary membrane, attenuation of transepithelial leukocyte and neutrophil migration, and down-regulation of proinflammatory cytokine and chemokine expression. Our work highlights a promising role for imaging-guided hAFS cell-based therapy in ALI.
authors.affiliation: Institute for Pediatric Research, Afﬁliated Children's Hospital, Soochow University, Suzhou, Jiangsu 215025, China.
authors.forename: Yunyun
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Institute of Functional Nano & Soft Materials (FUNSOM), Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou, Jiangsu 215123, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Xiang
authors.affiliation: Institute for Pediatric Research, Afﬁliated Children's Hospital, Soochow University, Suzhou, Jiangsu 215025, China.
authors.forename: He
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Stem Cell and Biomedical Material Key Laboratory of Jiangsu Province, State Key Laboratory Incubation Base, Soochow University, Suzhou, Jiangsu 215007, China.
authors.forename: Hansi
authors.initials: H
authors.lastname: Liang
authors.affiliation: Institute for Pediatric Research, Afﬁliated Children's Hospital, Soochow University, Suzhou, Jiangsu 215025, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Huang
authors.affiliation: Institute for Pediatric Research, Afﬁliated Children's Hospital, Soochow University, Suzhou, Jiangsu 215025, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute for Pediatric Research, Afﬁliated Children's Hospital, Soochow University, Suzhou, Jiangsu 215025, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Pan
authors.affiliation: Institute for Pediatric Research, Afﬁliated Children's Hospital, Soochow University, Suzhou, Jiangsu 215025, China.
authors.forename: Huiting
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Stem Cell and Biomedical Material Key Laboratory of Jiangsu Province, State Key Laboratory Incubation Base, Soochow University, Suzhou, Jiangsu 215007, China.
authors.forename: Xueguang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Academic Surgery, University College Cork, Cork University Hospital, Cork, Ireland.
authors.forename: Jiang Huai
authors.initials: JH
authors.lastname: Wang
authors.affiliation: Institute of Functional Nano & Soft Materials (FUNSOM), Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou, Jiangsu 215123, China.
authors.forename: Zhuang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Institute for Pediatric Research, Afﬁliated Children's Hospital, Soochow University, Suzhou, Jiangsu 215025, China. Electronic address: wj196312@vip.163.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
mesh_terms: D055371:Acute Lung Injury / Q000000981:diagnostic imaging* / Q000473:pathology / Q000628:therapy*; D000653:Amniotic Fluid / Q000166:cytology*; D000818:Animals; D005260:Female; D006801:Humans; D049408:Luminescent Agents / Q000737:chemistry*; D008163:Luminescent Measurements; D008168:Lung / Q000000981:diagnostic imaging / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000737:chemistry*; D061848:Optical Imaging; D011092:Polyethylene Glycols / Q000737:chemistry; D011094:Polyethyleneimine / Q000031:analogs & derivatives / Q000737:chemistry; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000737:chemistry / Q000166:cytology*
chemical_list: D049408:Luminescent Agents; C544016:poly(ethylene glycol)-co-poly(ethyleneimine); D011092:Polyethylene Glycols; D011094:Polyethyleneimine
keywords: Acute lung injury; Cell therapy; Human amniotic fluid stem cells; Sensitive tracking; Up-conversion nanoparticles