title: Response Patterns of GABAergic Neurons in the Anterior Piriform Cortex of Awake Mice.
abstract: Local inhibition by γ-amino butyric acid (GABA)-containing neurons is of vital importance for the operation of sensory cortices. However, the physiological response patterns of cortical GABAergic neurons are poorly understood, especially in the awake condition. Here, we utilized the recently developed optical tagging technique to specifically record GABAergic neurons in the anterior piriform cortex (aPC) in awake mice. The identified aPC GABAergic neurons were stimulated with robotic delivery of 32 distinct odorants, which covered a broad range of functional groups. We found that aPC GABAergic neurons could be divided into 4 types based on their response patterns. Type I, type II, and type III neurons displayed broad excitatory responses to test odorants with different dynamics. Type I neurons were constantly activated during odorant stimulation, whereas type II neurons were only transiently activated at the onset of odorant delivery. In addition, type III neurons displayed transient excitatory responses both at the onset and termination of odorant presentation. Interestingly, type IV neurons were broadly inhibited by most of the odorants. Taken together, aPC GABAergic neurons adopt different strategies to affect the cortical circuitry. Our results will allow for better understanding of the role of cortical GABAergic interneurons in sensory information processing.
authors.affiliation: Center for Neuron and Disease, Frontier Institute of Science and Technology, Xi'an Jiaotong University, Xi'an, Shaanxi 710054, China.|School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
authors.forename: Rongfeng
authors.initials: R
authors.lastname: Hu
authors.affiliation: National Institute of Biological Sciences, Beijing 102206, China.
authors.forename: Juen
authors.initials: J
authors.lastname: Zhang
authors.affiliation: National Institute of Biological Sciences, Beijing 102206, China.
authors.forename: Minmin
authors.initials: M
authors.lastname: Luo
authors.affiliation: School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
authors.forename: Ji
authors.initials: J
authors.lastname: Hu
mesh_terms: D000200:Action Potentials / Q000235:genetics / Q000502:physiology; D000818:Animals; D000075402:Channelrhodopsins / Q000235:genetics / Q000378:metabolism; D059330:GABAergic Neurons / Q000502:physiology*; D005968:Glutamate Decarboxylase / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008027:Light; D051379:Mice; D008822:Mice, Transgenic; D009433:Neural Inhibition / Q000502:physiology; D009812:Odorants; D055696:Olfactory Perception / Q000502:physiology*; D062308:Optogenetics; D066195:Piriform Cortex / Q000166:cytology*; D025341:Principal Component Analysis; D012903:Smell / Q000502:physiology; D050495:Vesicular Inhibitory Amino Acid Transport Proteins / Q000235:genetics / Q000378:metabolism; D014851:Wakefulness / Q000502:physiology*
chemical_list: D000075402:Channelrhodopsins; D050495:Vesicular Inhibitory Amino Acid Transport Proteins; C492244:Viaat protein, mouse; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D005968:Glutamate Decarboxylase; C402565:glutamate decarboxylase 1
keywords: cortical circuitry; head-fixed; multichannel recording; optical tagging; sensory processing