title: Label-free Gram-negative bacteria detection using bacteriophage-adhesin-coated long-period gratings.
abstract: This paper presents a novel application of a highly sensitive sensor based on long-period gratings (LPGs) coated with T4 bacteriophage adhesin for Gram-negative bacteria detection. We show here, that the sensor evidently recognizes Escherichia coli K-12 (PCM2560), whereas in the reference tests - ELISA and BIAcore - the results are questionable. For LPGs sensor the resonant wavelength shift observed for E. coli K-12 was approximately half of that measured for E.coli B (positive control). The BIAcore readings (RU) for E. coli K-12 were at 10% level of the signal obtained for E .coli B. These results confirm the improved sensitivity of the LPGs sensor. Moreover, we also show that application of adhesin may allow for efficient detection of E. coli O111 (PCM418), Klebsiella pneumoniae O1 (PCM1) and Yersinia enterocolitica O1 (PCM1879). The specificity of binding bacteria by the adhesin is discussed and it is determined by a distinct region of lipopolysaccharide receptors and/or by the presence of outer-membrane protein C in an outer membrane of Gram-negative bacteria.
authors.affiliation: Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wrocław Poland; ezuziak@iitd.pan.wroc.pl.
authors.forename: Ewa
authors.initials: E
authors.lastname: Brzozowska
authors.affiliation: Centre de Recherche en Photonique, Université du Québec en Outaouais, 101 rue Saint-Jean-Bosco, Gatineau, QC, J8X 3X7 Canada; National Institute of Telecommunications, Szachowa 1, 04-894 Warsaw Poland; Institute of Microelectronics and Optoelectronics, Warsaw University of Technology, Koszykowa 75, 00-662 Warsaw Poland; mkoba@elka.pw.edu.pl.
authors.forename: Marcin
authors.initials: M
authors.lastname: Koba
authors.affiliation: Institute of Microelectronics and Optoelectronics, Warsaw University of Technology, Koszykowa 75, 00-662 Warsaw Poland.
authors.forename: Mateusz
authors.initials: M
authors.lastname: Śmietana
authors.affiliation: Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wrocław Poland.
authors.forename: Sabina
authors.initials: S
authors.lastname: Górska
authors.affiliation: Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wrocław Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Janik
authors.affiliation: Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wrocław Poland.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Gamian
authors.affiliation: Centre de Recherche en Photonique, Université du Québec en Outaouais, 101 rue Saint-Jean-Bosco, Gatineau, QC, J8X 3X7 Canada.
authors.forename: Wojtek J
authors.initials: WJ
authors.lastname: Bock
keywords: (060.2370) Fiber optics sensors; (170.1420) Biology