title: Lipid Homeostasis and Ligands for Liver X Receptors: Identification and Characterization.
abstract: Screening of bona fide ligands for nuclear receptors is a real tour de force as the identified molecules are supposed to be able to activate the targeted proteins in cell culture as well as in vivo. Indeed orphan nuclear receptors are putative pharmacologically targets for various diseases. It is thus necessary to have quick and reproductive systems that help in identifying new ligands, agonist or antagonist, before using them in vivo in animal models to check for secondary effects. Here, we describe the transient transfections (homologous and heterologous) used for the screening of ligands for liver X receptor α (LXRα, NR1H3) in HeLa cells.
authors.affiliation: Université Clermont Auvergne, Université Blaise Pascal, Génétique Reproduction et Développement, 63000, Clermont-Ferrand, France. j-marc.lobaccaro@univ-bpclermont.fr.|CNRS, UMR 6293, GReD, 24 avenue des Landais, BP80026, 63177, Aubière, France. j-marc.lobaccaro@univ-bpclermont.fr.|INSERM, UMR 1103, GReD, 63177, Aubière, France. j-marc.lobaccaro@univ-bpclermont.fr.|Centre de Recherche en Nutrition Humaine d'Auvergne, 63000, Clermont-Ferrand, France. j-marc.lobaccaro@univ-bpclermont.fr.
authors.forename: Jean-Marc A
authors.initials: JM
authors.lastname: Lobaccaro
authors.affiliation: Université Clermont Auvergne, Université Blaise Pascal, Génétique Reproduction et Développement, 63000, Clermont-Ferrand, France.|CNRS, UMR 6293, GReD, 24 avenue des Landais, BP80026, 63177, Aubière, France.|INSERM, UMR 1103, GReD, 63177, Aubière, France.|Centre de Recherche en Nutrition Humaine d'Auvergne, 63000, Clermont-Ferrand, France.
authors.forename: Claude
authors.initials: C
authors.lastname: Beaudoin
authors.affiliation: Université Clermont Auvergne, Université Blaise Pascal, Génétique Reproduction et Développement, 63000, Clermont-Ferrand, France.|CNRS, UMR 6293, GReD, 24 avenue des Landais, BP80026, 63177, Aubière, France.|INSERM, UMR 1103, GReD, 63177, Aubière, France.|Centre de Recherche en Nutrition Humaine d'Auvergne, 63000, Clermont-Ferrand, France.|University of Koudougou, Burkina, Faso.
authors.forename: Bagora
authors.initials: B
authors.lastname: Bayala
authors.affiliation: Université Clermont Auvergne, Université Blaise Pascal, Génétique Reproduction et Développement, 63000, Clermont-Ferrand, France.|CNRS, UMR 6293, GReD, 24 avenue des Landais, BP80026, 63177, Aubière, France.|INSERM, UMR 1103, GReD, 63177, Aubière, France.|Centre de Recherche en Nutrition Humaine d'Auvergne, 63000, Clermont-Ferrand, France.
authors.forename: Silvère
authors.initials: S
authors.lastname: Baron
authors.affiliation: Université Clermont Auvergne, Université Blaise Pascal, Génétique Reproduction et Développement, 63000, Clermont-Ferrand, France. amalia.trousson@univ-bpclermont.fr.|CNRS, UMR 6293, GReD, 24 avenue des Landais, BP80026, 63177, Aubière, France. amalia.trousson@univ-bpclermont.fr.|INSERM, UMR 1103, GReD, 63177, Aubière, France. amalia.trousson@univ-bpclermont.fr.|Centre de Recherche en Nutrition Humaine d'Auvergne, 63000, Clermont-Ferrand, France. amalia.trousson@univ-bpclermont.fr.
authors.forename: Amalia
authors.initials: A
authors.lastname: Trousson
mesh_terms: D000818:Animals; D006367:HeLa Cells; D057166:High-Throughput Screening Assays / Q000379:methods*; D006706:Homeostasis; D006801:Humans; D008024:Ligands; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry; D000071518:Liver X Receptors / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008156:Luciferases / Q000378:metabolism; D014162:Transfection / Q000379:methods*
chemical_list: D008024:Ligands; D008055:Lipids; D000071518:Liver X Receptors; D008156:Luciferases
keywords: LXR; Ligand screening; Oxysterols; Pharmacological target; Transient transfection