title: Can Coenzyme Q10 supplementation protect the ovarian reserve against oxidative damage?
abstract: OBJECTIVE
  We investigated antioxidant effects of CoQ10 supplementation on the prevention of OS-induced ovarian damage and to evaluate the protective effect of such supplementation against OS-related DNA damage.
  
  
  METHODS
  Twenty-four adult female Sprague-Dawley rats were randomly divided into three groups (8 rats per group): group 1 (control): saline, ip, and orally; group 2 (cisplatin group): cisplatin, 4.5 mg/kg ip, two times with an interval of 7 days; and group 3 (cisplatin + CoQ10 group): cisplatin, 4.5 mg/kg ip, two times with an interval of 7 days, and 24 h before cisplatin, 150 mg/kg/day orally in 1 mL of saline daily for 14 days. Serum concentrations of anti-Mullerian hormone (AMH), number of AMH-positive follicles, the assessment of the intensity of 8'OHdG immunoreactivity, the primordial, antral and atretic follicle counts in the ovary were assessed.
  
  
  RESULTS
  The mean serum AMH concentrations were 1.3 ± 0.19, 0.16 ± 0.03, and 0.27 ± 0.20 ng/mL in groups 1, 2, and 3, respectively (p < 0.01). Serum AMH levels were significantly higher in group 1 compared to groups 2 and 3 (p < 0.01 and p = 0.01, respectively). There was a statistically significant difference in AMH-positive follicle count between the groups (p < 0.01). Group 1 showed higher numbers of AMH-positive granulosa cells compared to group 2 (p = 0.01). A significant difference was found in the primordial, the atretic, and antral follicle counts between the three groups (p < 0.01, p < 0.01, and p < 0.01, respectively). The atretic follicle count was significantly lower in the cisplatin plus CoQ10 group compared to the cisplatin group (p < 0.01). The antral follicle counts were significantly higher in the cisplatin plus CoQ10 group compared with the cisplatin group (p < 0.01). There was a statistically significant difference in the intensity of staining of the follicles that were positive for anti-8'OHdG between the groups (p = 0.02). Group 1 showed a significant lower intensity of staining of the follicles positive for anti-8'OHdG compared with group 2 (p = 0.03).
  
  
  CONCLUSIONS
  CoQ10 supplementation may protect ovarian reserve by counteracting both mitochondrial ovarian ageing and physiological programmed ovarian ageing although the certain effect of OS in female infertility is not clearly known.
authors.affiliation: Department of Obstetrics and Gynecology, Bezmialem University Faculty of Medicine, İstanbul, Turkey. POZCAN@partners.org.|Division of Reproductive Endocrinology and Infertility, Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women's Hospital, Harvard Medical School, 75 Francis Street, Boston, MA, 02115, USA. POZCAN@partners.org.
authors.forename: Pınar
authors.initials: P
authors.lastname: Özcan
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, Yeditepe University, Istanbul, Turkey.
authors.forename: Cem
authors.initials: C
authors.lastname: Fıçıcıoğlu
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, Yeditepe University, Istanbul, Turkey.
authors.forename: Ozge
authors.initials: O
authors.lastname: Kizilkale
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, Yeditepe University, Istanbul, Turkey.
authors.forename: Mert
authors.initials: M
authors.lastname: Yesiladali
authors.affiliation: Department of Histology and Embryology, Bezmialem University Faculty of Medicine, İstanbul, Turkey.
authors.forename: Olgu Enis
authors.initials: OE
authors.lastname: Tok
authors.affiliation: Department of Pathology, Faculty of Medicine, Yeditepe University, Istanbul, Turkey.
authors.forename: Ferda
authors.initials: F
authors.lastname: Ozkan
authors.affiliation: Department of Histology and Embryology, Bezmialem University Faculty of Medicine, İstanbul, Turkey.
authors.forename: Mukaddes
authors.initials: M
authors.lastname: Esrefoglu
mesh_terms: D000328:Adult; D000375:Aging / Q000187:drug effects / Q000473:pathology; D000818:Animals; D054304:Anti-Mullerian Hormone / Q000097:blood; D000975:Antioxidants / Q000008:administration & dosage; D019587:Dietary Supplements; D005260:Female; D006801:Humans; D065851:Ovarian Reserve / Q000187:drug effects*; D010053:Ovary / Q000187:drug effects* / Q000254:growth & development; D018384:Oxidative Stress / Q000187:drug effects*; D011247:Pregnancy; D051381:Rats; D014451:Ubiquinone / Q000008:administration & dosage / Q000031:analogs & derivatives*
chemical_list: D000975:Antioxidants; D014451:Ubiquinone; D054304:Anti-Mullerian Hormone; C024989:coenzyme Q10
keywords: 8'OHdG; AMH; Coenzyme Q10; Ovarian reserve; Oxidative damage