title: Preparation of Extracellular Matrices Produced by Cultured and Primary Fibroblasts.
abstract: Fibroblasts secrete and organize extracellular matrix (ECM), which provides structural support for their adhesion, migration, and tissue organization, besides regulating cellular functions such as growth and survival. Cell-to-matrix interactions are vital for vertebrate development. Disorders in these processes have been associated with fibrosis, developmental malformations, cancer, and other diseases. This unit describes a method for preparing a three-dimensional matrix derived from fibroblastic cells; the matrix is three-dimensional, cell and debris free, and attached to a two-dimensional culture surface. Cell adhesion and spreading are normal on these matrices. This matrix can also be compressed into a two-dimensional matrix and solubilized to study the matrix biochemically. © 2016 by John Wiley & Sons, Inc.
authors.affiliation: Fox Chase Cancer Center, Philadelphia, Pennsylvania.
authors.forename: Janusz
authors.initials: J
authors.lastname: Franco-Barraza
authors.affiliation: Fox Chase Cancer Center, Philadelphia, Pennsylvania.
authors.forename: Dorothy A
authors.initials: DA
authors.lastname: Beacham
authors.affiliation: Fox Chase Cancer Center, Philadelphia, Pennsylvania.
authors.forename: Michael D
authors.initials: MD
authors.lastname: Amatangelo
authors.affiliation: Fox Chase Cancer Center, Philadelphia, Pennsylvania.
authors.forename: Edna
authors.initials: E
authors.lastname: Cukierman
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods*; D002469:Cell Separation; D048430:Cell Shape; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D010641:Phenotype; D012710:Serum Albumin, Bovine / Q000378:metabolism; D012995:Solubility; D013194:Staining and Labeling
chemical_list: D000199:Actins; C544013:alpha-smooth muscle actin, mouse; D012710:Serum Albumin, Bovine
keywords: 3D cell culture; cell-derived extracellular matrices; desmoplasia; myofibroblasts; primary fibroblasts; tumor- or cancer-associated fibroblasts