title: Prediction of the Risk for Essential Hypertension among Carriers of C825T Genetic Polymorphism of G Protein β3 (GNB3) Gene.
abstract: BACKGROUND
  The guanine nucleotide-binding protein beta polypeptide 3 (GNB3) 825T allele encodes a product that enhances the activation of heterotrimeric G proteins, which is associated with the occurrence of the splice variant Gβ3 s that could play a role in vascular reactivity and hyperproliferation of smooth muscle cells, that makes such proteins attractive candidate gene products for susceptibility to essential hypertension (EH).
  
  
  OBJECTIVE
  To predict the risk for EH in individuals with C825T genetic polymorphism of G protein β3 gene.
  
  
  METHODS
  The study consisted of 222 normotensive individuals and 216 hypertensive patients. Individuals were genotyped for C825T genetic polymorphism of G protein β3 gene rs5443 by using restriction fragment length polymorphism.
  
  
  RESULTS
  Frequencies of C and T alleles were 58.1% and 41.9%, respectively, in the control group compared with 47.7% and 52.3%, respectively, in the hypertensive group. The carriers of rs5443 (T) allele exhibited a significant greater risk for EH compared with the carriers of rs5443 (C) allele (odds ratio = 1.5, 95% confidence interval = 1.2-2.0).
  
  
  CONCLUSIONS
  T allele is a risk factor for EH in the Egyptian population, which may be used as a prognostic and a therapeutic target of prophylaxis.
authors.affiliation: Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
authors.forename: Neveen Salah
authors.initials: NS
authors.lastname: El Din Hemimi
authors.affiliation: Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
authors.forename: Amal A
authors.initials: AA
authors.lastname: Mansour
authors.affiliation: Endocrine Unit, Internal Medicine Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
authors.forename: Mona Mohamed
authors.initials: MM
authors.lastname: Abdelsalam
keywords: BseDI; G protein; PCR; RFLP; essential hypertension; polymorphism