title: The proteome of the infectious bronchitis virus  Beau-R virion.
abstract: Infectious bronchitis is a highly contagious respiratory disease of poultry caused by the coronavirus infectious bronchitis virus (IBV). It was thought that coronavirus virions were composed of three major viral structural proteins until investigations of other coronaviruses showed that the virions also include viral non-structural and genus-specific accessory proteins as well as host-cell proteins. To study the proteome of IBV virions, virus was grown in embryonated chicken eggs, purified by sucrose-gradient ultracentrifugation and analysed by mass spectrometry. Analysis of three preparations of purified IBV yielded the three expected structural proteins plus 35 additional virion-associated host proteins. The virion-associated host proteins had a diverse range of functional attributions, being involved in cytoskeleton formation, RNA binding and protein folding pathways. Some of these proteins were unique to this study, while others were found to be orthologous to proteins identified in severe acute respiratory syndrome coronavirus virions and also virions from a number of other RNA and DNA viruses.
authors.affiliation: Compton Laboratory, Compton, The Pirbright Institute, Newbury RG20 7NN, UK.|School of Biological Sciences, University of Reading, Whiteknights, Reading RG6 6AJ, UK.
authors.forename: Stuart D
authors.initials: SD
authors.lastname: Dent
authors.affiliation: Institute of Infection and Global Health, University of Liverpool, Liverpool L3 5RF, UK.
authors.forename: Dong
authors.initials: D
authors.lastname: Xia
authors.affiliation: Institute of Infection and Global Health, University of Liverpool, Liverpool L3 5RF, UK.|Faculty of Natural Sciences, University of Keele, Keele ST5 5BG, UK.
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Wastling
authors.affiliation: School of Biological Sciences, University of Reading, Whiteknights, Reading RG6 6AJ, UK.
authors.forename: Benjamin W
authors.initials: BW
authors.lastname: Neuman
authors.affiliation: Compton Laboratory, Compton, The Pirbright Institute, Newbury RG20 7NN, UK.
authors.forename: Paul
authors.initials: P
authors.lastname: Britton
authors.affiliation: Compton Laboratory, Compton, The Pirbright Institute, Newbury RG20 7NN, UK.
authors.forename: Helena J
authors.initials: HJ
authors.lastname: Maier
mesh_terms: D000482:Allantois / Q000821:virology; D000818:Animals; D002642:Chick Embryo; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D001351:Infectious bronchitis virus / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D013058:Mass Spectrometry; D020543:Proteome; D013047:Specific Pathogen-Free Organisms; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D014771:Virion / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D020543:Proteome; D014764:Viral Proteins