title: Size-isolation of ultrasound-mediated phase change perfluorocarbon droplets using differential centrifugation.
abstract: Perfluorocarbon droplets that are capable of an ultrasound-mediated phase transition have applications in diagnostic and therapeutic ultrasound. Techniques to modify the droplet size distribution are of interest because of the size-dependent acoustic response of the droplets. Differential centrifugation has been used to isolate specific sizes of microbubbles. In this work, differential centrifugation was employed to isolate droplets with diameters between 1 and 3 μm and 2 and 5 μm from an initially polydisperse distribution. Further, an empirical model was developed for predicting the droplet size distribution following differential centrifugation and to facilitate the selection of centrifugation parameters for obtaining desired size distributions.
authors.affiliation: Department of Internal Medicine, University of Cincinnati, Cincinnati, Ohio 45267, USA.
authors.forename: Karla P
authors.initials: KP
authors.lastname: Mercado
authors.affiliation: Department of Internal Medicine, University of Cincinnati, Cincinnati, Ohio 45267, USA.
authors.forename: Kirthi
authors.initials: K
authors.lastname: Radhakrishnan
authors.affiliation: Biomedical Engineering Program, University of Cincinnati, Cincinnati, Ohio 45267, USA.
authors.forename: Kyle
authors.initials: K
authors.lastname: Stewart
authors.affiliation: Biomedical Engineering Program, University of Cincinnati, Cincinnati, Ohio 45267, USA.
authors.forename: Lindsay
authors.initials: L
authors.lastname: Snider
authors.affiliation: Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, Missouri 63130, USA karlapatricia.mercado@uc.edu, kirthi.radhakrishnan@gmail.com, stewake@mail.uc.edu, sniderlm@mail.uc.edu, devin.ryan514@gmail.com, kevin.haworth@uc.edu.
authors.forename: Devin
authors.initials: D
authors.lastname: Ryan
authors.affiliation: Department of Internal Medicine, University of Cincinnati, Cincinnati, Ohio 45267, USA.
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Haworth
mesh_terms: D002498:Centrifugation / Q000379:methods*; D005466:Fluorocarbons / Q000737:chemistry*; D008962:Models, Theoretical; D010316:Particle Size; D044367:Phase Transition; D014465:Ultrasonics / Q000379:methods*; D010420:Pentanes
chemical_list: D005466:Fluorocarbons; C008806:perfluoropentane; D010420:Pentanes