title: Monkey Viperin Restricts Porcine Reproductive and Respiratory Syndrome Virus Replication.
abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) is an important pathogen which causes huge economic damage globally in the swine industry. Current vaccination strategies provide only limited protection against PRRSV infection. Viperin is an interferon (IFN) stimulated protein that inhibits some virus infections via IFN-dependent or IFN-independent pathways. However, the role of viperin in PRRSV infection is not well understood. In this study, we cloned the full-length monkey viperin (mViperin) complementary DNA (cDNA) from IFN-α-treated African green monkey Marc-145 cells. It was found that the mViperin is up-regulated following PRRSV infection in Marc-145 cells along with elevated IRF-1 gene levels. IFN-α induced mViperin expression in a dose- and time-dependent manner and strongly inhibits PRRSV replication in Marc-145 cells. Overexpression of mViperin suppresses PRRSV replication by blocking the early steps of PRRSV entry and genome replication and translation but not inhibiting assembly and release. And mViperin co-localized with PRRSV GP5 and N protein, but only interacted with N protein in distinct cytoplasmic loci. Furthermore, it was found that the 13-16 amino acids of mViperin were essential for inhibiting PRRSV replication, by disrupting the distribution of mViperin protein from the granular distribution to a homogeneous distribution in the cytoplasm. These results could be helpful in the future development of novel antiviral therapies against PRRSV infection.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Jianyu
authors.initials: J
authors.lastname: Fang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Bai
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Qiaoya
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.|Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Yangzhou, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Jiang
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects; D055785:Gene Knockdown Techniques; D016679:Genome, Viral / Q000235:genetics; D000882:Haplorhini; D016898:Interferon-alpha / Q000494:pharmacology; D019316:Porcine respiratory and reproductive syndrome virus / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D021381:Protein Transport; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D014764:Viral Proteins / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D016898:Interferon-alpha; D011506:Proteins; D012333:RNA, Messenger; D014764:Viral Proteins