title: Genome-wide investigation of the NAC transcription factor family in melon (Cucumis melo L.) and their expression analysis under salt stress.
abstract: 82 melon NAC (CmNAC) genes were identified in melon. We putatively identified the function of CmNAC gene in melon under salt stress. NAC transcription factor proteins play important roles in many biological processes, including plant development and stress responses. To date, few full-length melon NAC proteins have been identified. In this study, 82 melon NAC (CmNAC) genes were identified in the Cucumis melo L. genome. By interrogating our cDNA libraries and transcriptome data from melon under salt stress, and comparison of their phylogenetic relationship with Arabidopsis NAC salt stress-related genes, we putatively identified that the fourth clade of CmNAC genes were involved in the salt stress response, especially the second clade of the group IV of the phylogenetic tree. Expression analysis confirmed that eleven of the twelve CmNAC genes from the group IV were induced in melon seedling roots by salt stress; the other gene was down regulated by salt stress. The expression of CmNAC14 continually increased in 12 h under salt stress, and was selected for transformation into Arabidopsis for functional verification. Overexpression of CmNAC14 increased the sensitivity of transgenic Arabidopsis lines to salt stress, which were simultaneously demonstrated by reduced expression of abiotic stress-response genes and variation in several physiological indices. This study increases our knowledge and may enable further characterization of the roles of CmNAC family in the response to salt stress.
authors.affiliation: Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China.|Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai, China.
authors.forename: Shiwei
authors.initials: S
authors.lastname: Wei
authors.affiliation: Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China.|Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai, China.
authors.forename: Liwei
authors.initials: L
authors.lastname: Gao
authors.affiliation: Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China.|Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai, China.
authors.forename: Yidong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China.|Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai, China.
authors.forename: Furong
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China.|Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China. danfenggrace@gmail.com.|Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai, China. danfenggrace@gmail.com.
authors.forename: Danfeng
authors.initials: D
authors.lastname: Huang
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D032461:Chromosomes, Plant / Q000235:genetics; D028463:Cucumis melo / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D015723:Gene Library; D020131:Genes, Duplicate; D017343:Genes, Plant; D018745:Genome, Plant; D058977:Molecular Sequence Annotation; D005810:Multigene Family; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics; D030821:Plants, Genetically Modified; D036226:Seedlings / Q000187:drug effects / Q000235:genetics; D012965:Sodium Chloride / Q000494:pharmacology*; D013312:Stress, Physiological / Q000187:drug effects* / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D059467:Transcriptome / Q000187:drug effects / Q000235:genetics
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors; D012965:Sodium Chloride
keywords: Bioinformatic analysis; Functional characterization; Melon; NAC; Salt stress