title: The effect of berberine hydrochloride on Enterococcus faecalis biofilm formation and dispersion in vitro.
abstract: Enterococcus faecalis (E. faecalis) is one of the major causes of biofilm infections. Berberine hydrochloride (BBH) has diverse pharmacological effects; however, the effects and mechanisms of BBH on E. faecalis biofilm formation and dispersion have not been reported. In this study, 99 clinical isolates from the urine samples of patients with urinary tract infections (UTIs) were collected and identified. Ten strains of E. faecalis with biofilm formation ability were studied. BBH inhibited E. faecalis biofilm formation and promoted the biofilm dispersion of E. faecalis. In addition, sortase A and esp expression levels were elevated during early E. faecalis biofilm development, whereas BBH significantly reduced their expression levels. The results of this study indicated that BBH effectively prevents biofilm formation and promotes biofilm dispersion in E. faecalis, most likely by inhibiting the expressions of sortase A and esp.
authors.affiliation: Department of Medicine Clinical Laboratory, the Third Xiangya Hospital of Central South University, Changsha 410013, Hunan, PR China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Medicine Clinical Laboratory, the Third Xiangya Hospital of Central South University, Changsha 410013, Hunan, PR China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Bu
authors.affiliation: Department of Medicine Clinical Laboratory, the Third Xiangya Hospital of Central South University, Changsha 410013, Hunan, PR China.
authors.forename: Huan
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Medicine Clinical Laboratory, the Third Xiangya Hospital of Central South University, Changsha 410013, Hunan, PR China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Medicine Clinical Laboratory, the Third Xiangya Hospital of Central South University, Changsha 410013, Hunan, PR China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: She
authors.affiliation: Department of Medicine Clinical Laboratory, the Third Xiangya Hospital of Central South University, Changsha 410013, Hunan, PR China.
authors.forename: Ruichen
authors.initials: R
authors.lastname: Tan
authors.affiliation: Department of Medicine Clinical Laboratory, the Third Xiangya Hospital of Central South University, Changsha 410013, Hunan, PR China. Electronic address: wuyong_xy@126.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wu
mesh_terms: D019881:Aminoacyltransferases / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D001599:Berberine / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D003546:Cysteine Endopeptidases / Q000096:biosynthesis; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000302:isolation & purification / Q000502:physiology; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D008565:Membrane Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D014552:Urinary Tract Infections / Q000382:microbiology; D014556:Urine / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D008565:Membrane Proteins; C116707:enterococcal surface protein, esp; D001599:Berberine; D019881:Aminoacyltransferases; C120542:sortase A; D003546:Cysteine Endopeptidases
keywords: Berberine hydrochloride; Biofilm; Confocal laser scanning microscopy; Enterococcus faecalis; esp; sortase A