title: Temperature regulates methane production through the function centralization of microbial community in anaerobic digestion.
abstract: Temperature is crucial for the performance of anaerobic digestion process. In this study of anaerobic digestion of swine manure, the relationship between the microbial gene expression and methane production at different temperatures (25-55°C) was revealed through metatranscriptomic analysis. Daily methane production and total biogas production increased with temperature up to 50°C, but decreased at 55°C. The functional gene expression showed great variation at different temperatures. The function centralization (opposite to alpha-diversity), assessed by the least proportions of functional pathways contributing for at least 50% of total reads positively correlated to methane production. Temperature regulated methane production probably through reducing the diversity of functional pathways, but enhancing central functional pathways, so that most of cellular activities and resource were invested in methanogenesis and related pathways, enhancing the efficiency of conversion of substrates to methane. This research demonstrated the importance of function centralization for efficient system functioning.
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, CAS, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Lin
authors.affiliation: Laboratory of Microbial Ecology and Technology, Ghent University, Coupure Links 653, B-9000 Gent, Belgium.
authors.forename: Jo
authors.initials: J
authors.lastname: De Vrieze
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, CAS, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
authors.forename: Guihua
authors.initials: G
authors.lastname: He
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, CAS, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
authors.forename: Xiangzhen
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Environmental and Applied Microbiology, CAS, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China. Electronic address: Lijb@cib.ac.cn.
authors.forename: Jiabao
authors.initials: J
authors.lastname: Li
mesh_terms: D000693:Anaerobiosis; D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D056804:Biofuels; D019149:Bioreactors / Q000382:microbiology; D016000:Cluster Analysis; D005232:Fatty Acids, Volatile / Q000032:analysis; D008372:Manure; D053858:Metabolic Networks and Pathways; D008697:Methane / Q000096:biosynthesis*; D010802:Phylogeny; D013552:Swine; D013696:Temperature
chemical_list: D056804:Biofuels; D005232:Fatty Acids, Volatile; D008372:Manure; D008697:Methane
keywords: Anaerobic digestion; Function centralization; Functional diversity; Metatranscriptome; Temperature