title: Determination of ibuprofen enantiomers in human plasma by HPLC-MS/MS: validation and application in neonates.
abstract: OBJECTIVE
  An adaptive method to determine ibuprofen enantiomers with limited volume of plasma required is necessary for investigating PK of ibuprofen in neonates.
  
  
  RESULTS
  Enantiomer separation was achieved on a Lux cellulose 3 column with mobile phase consisting of methanol water (85:15, v/v) and formic acid (0.0075%) at isocratic rate of 0.2 ml/min. Calibration curve is linear for each enantiomer at the range of 0.1-60 μg/ml. Validation was conducted and results met requirements regarding to intra- and inter-run precision, accuracy and recovery. No matrix effect or interference was observed from neonatal plasma or comedications. Only 20 μl of plasma was requested in this study.
  
  
  CONCLUSIONS
  This assay was specific and reliable to quantify ibuprofen enantiomers in neonate plasma.
authors.affiliation: Department of Pharmacy, Children's Hospital of Fudan University, 399 Wanyuan Road, Shanghai 201102, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Chen
authors.affiliation: Department of Pharmacy, Children's Hospital of Fudan University, 399 Wanyuan Road, Shanghai 201102, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Pharmacy, Children's Hospital of Fudan University, 399 Wanyuan Road, Shanghai 201102, China.
authors.forename: Jinmiao
authors.initials: J
authors.lastname: Lu
authors.affiliation: Central Laboratory, Shanghai Xuhui Central Hospital, Shanghai, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Yu
authors.affiliation: Department of Neonatology, Children's Hospital of Fudan University, Shanghai, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Pharmacy, Children's Hospital of Fudan University, 399 Wanyuan Road, Shanghai 201102, China.
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Li
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis / Q000097:blood* / Q000302:isolation & purification; D002138:Calibration; D002482:Cellulose / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007052:Ibuprofen / Q000032:analysis / Q000097:blood* / Q000302:isolation & purification; D007231:Infant, Newborn; D057230:Limit of Detection; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D002482:Cellulose; D007052:Ibuprofen
keywords: HPLC–MS/MS; enantiomers; ibuprofen; interference; neonate