title: De novo transcriptome assembly of two Vigna angularis varieties collected from Korea.
abstract: The adzuki bean (Vigna angularis), a member of the family Fabaceae, is widely grown in Asia, from East Asia to the Himalayas. The adzuki bean is known as an ingredient that adds sweetness to diverse desserts made in Eastern Asian countries. Libraries prepared from two V. angularis varieties referred to as Taejin Black and Taejin Red were paired-end sequenced using the Illumina HiSeq 2000 system. The raw data in this study can be available in NCBI SRA database with accession numbers of SRR3406660 and SRR3406553. After de novo transcriptome assembly using Trinity, we obtained 324,219 and 280,056 transcripts from Taejin Black and Taejin Red, respectively. We predicted a total of 238,321 proteins and 179,519 proteins for Taejin Black and Taejin Red, respectively, by the TransDecoder program. We carried out BLASTP on the predicted proteins against the Swiss-Prot protein sequence database to predict the putative functions of identified proteins. Taken together, we provide transcriptomes of two adzuki bean varieties by RNA-Seq, which might be usefully applied to generate molecular markers.
authors.affiliation: Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Yeonhwa
authors.initials: Y
authors.lastname: Jo
authors.affiliation: College of Crop Protection and Agronomy, Qingdao Agricultural University, Qingdao, Shandong 266109, China.
authors.forename: Sen
authors.initials: S
authors.lastname: Lian
authors.affiliation: The Taejin Genome Institute, Gadam-gil 61, Hoengseong 25239, Republic of Korea.
authors.forename: Jin Kyong
authors.initials: JK
authors.lastname: Cho
authors.affiliation: Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Hoseong
authors.initials: H
authors.lastname: Choi
authors.affiliation: Crop Foundation Division, National Institute of Crop Science, RDA, Wanju 55365, Republic of Korea.
authors.forename: Sang-Min
authors.initials: SM
authors.lastname: Kim
authors.affiliation: Crop Foundation Division, National Institute of Crop Science, RDA, Wanju 55365, Republic of Korea.
authors.forename: Sun-Lim
authors.initials: SL
authors.lastname: Kim
authors.affiliation: Crop Foundation Division, National Institute of Crop Science, RDA, Wanju 55365, Republic of Korea.
authors.forename: Bong Choon
authors.initials: BC
authors.lastname: Lee
authors.affiliation: Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea; The Taejin Genome Institute, Gadam-gil 61, Hoengseong 25239, Republic of Korea.
authors.forename: Won Kyong
authors.initials: WK
authors.lastname: Cho
keywords: Adzuki bean; RNA-Seq; Transcriptome; Variety; Vigna angularis