title: Lacrimal gland primary acinar cell culture: the role of insulin.
abstract: OBJECTIVE
  The goal of the present study was to establish a protocol for primary culture of lacrimal gland acinar cells (LGACs) and to assess the effect of adding insulin to the culture media.
  
  
  METHODS
  LGACs were isolated and cultured from lacrimal glands of Wistar male rats. The study outcomes included cell number, viability, and peroxidase release over time and in response to three concentrations of insulin (0.5, 5.0, and 50.0 μg/mL).
  
  
  RESULTS
  In LGAC primary culture, cells started to form clusters by day 3. There was a time-response pattern of peroxidase release, which rose by day 6, in response to carbachol. Culture viability lasted for 12 days. An insulin concentration of 5.0 μg/mL in the culture medium resulted in higher viability and secretory capacity.
  
  
  CONCLUSIONS
  The present method simplifies the isolation and culture of LGACs. The data confirmed the relevance of adding insulin to maintain LGACs in culture.
authors.affiliation: Department of Ophthalmology, Otorhinolaryngology and Head & Neck Surgery, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Leonardo Tannus
authors.initials: LT
authors.lastname: Malki
authors.affiliation: Department of Ophthalmology, Otorhinolaryngology and Head & Neck Surgery, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Ana Carolina
authors.initials: AC
authors.lastname: Dias
authors.affiliation: Department of Ophthalmology, Otorhinolaryngology and Head & Neck Surgery, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Angelica Gobbi
authors.initials: AG
authors.lastname: Jorge
authors.affiliation: Department of Ophthalmology, Otorhinolaryngology and Head & Neck Surgery, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Carolina Maria
authors.initials: CM
authors.lastname: Módulo
authors.affiliation: Department of Ophthalmology, Otorhinolaryngology and Head & Neck Surgery, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Eduardo Melani
authors.initials: EM
authors.lastname: Rocha
mesh_terms: D061354:Acinar Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D002217:Carbachol / Q000378:metabolism; D002452:Cell Count / Q000379:methods; D002469:Cell Separation / Q000379:methods; D007328:Insulin / Q000378:metabolism / Q000494:pharmacology*; D007765:Lacrimal Apparatus / Q000166:cytology* / Q000378:metabolism; D008297:Male; D009195:Peroxidase / Q000378:metabolism; D061251:Primary Cell Culture / Q000592:standards*; D017208:Rats, Wistar
chemical_list: D007328:Insulin; D002217:Carbachol; D009195:Peroxidase