title: Analysis of microRNA Levels in Intestinal Epithelial Cells.
abstract: The field of microRNA (miRNA) research is expanding rapidly with the crucial role of miRNAs in almost every biological process and their implication in many diseases. The role of miRNAs in modulating inflammatory responses in the gut has attracted many research groups including us. Here, we first briefly summarize our current understanding of the role of miRNAs in maintaining and regulating gut physiopathology and in inflammatory bowel diseases. We then describe in detail our techniques to analyze miRNA levels with notes that we have collected and summarized during our experiments.
authors.affiliation: UMR 1071 Inserm, University of Auvergne, 28 Place Henri Dunant, Clermont-Ferrand, 63001, France. hang.nguyen@udamail.fr.
authors.forename: Hang Thi Thu
authors.initials: HT
authors.lastname: Nguyen
mesh_terms: D002460:Cell Line; D004847:Epithelial Cells / Q000737:chemistry / Q000382:microbiology; D004926:Escherichia coli / Q000502:physiology; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000235:genetics; D007422:Intestines / Q000737:chemistry / Q000382:microbiology*; D035683:MicroRNAs / Q000032:analysis*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*
chemical_list: D035683:MicroRNAs
keywords: Inflammatory bowel disease; Total RNA extraction; cDNA analysis; microRNA; qRT-PCR