title: Antiosteoporotic effect of Petroselinum crispum, Ocimum basilicum and Cichorium intybus L. in glucocorticoid-induced osteoporosis in rats.
abstract: BACKGROUND
  Glucocorticoid-induced osteoporosis (GIO) is one of the serious side effects which have become the most common secondary osteoporosis. The purpose of this study is to evaluate the effect of aqueous extract of parsley, basil and chicory on glucocorticoid-induced osteoporosis in rats.
  
  
  METHODS
  Fifty Female rats were divided into five groups and treated for 8 weeks as follow: group 1 served as control; group (2) subcutaneously injected with 0.1 mg/kg b. wt. dexamethasone dissolved in saline; group 3 received similar dose of dexamethasone together with aqueous parsley extract in a dose of 2 g/kg b. wt.; group 4 received similar dose of dexamethasone together with 400 mg/kg b. wt. aqueous basil extract and group 5 received similar dose of dexamethasone together with 100 mg/kg b. wt. aqueous chicory extract.
  
  
  RESULTS
  The dexamethasone group showed a significant decrease in serum E2, Ca, P levels and significant decrease in total BMD, BMC and a significant increase in serum PTH, ALP and ACP. Bone TBARs was significantly increased while GSH, antioxidant enzymes were significantly decreased. These changes were attenuated by parsley, basil and chicory extracts in the group 3, 4 and 5 respectively.
  
  
  CONCLUSIONS
  Aqueous extracts of parsley, basil and chicory showed bone protection against glucocorticoid-induced in rats. From our results, we concluded that chicory has a potent protective effect more than parsley and basil due to containing flavonoids and inulin.
authors.affiliation: Chemistry Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt. Walaahozayen@hotmail.com.|Biotechnology Department, Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef University, Beni-Suef, Egypt. Walaahozayen@hotmail.com.
authors.forename: Walaa G
authors.initials: WG
authors.lastname: Hozayen
authors.affiliation: Chemistry Department, Faculty of Science, Cairo University, Giza, Egypt.
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: El-Desouky
authors.affiliation: Chemistry Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt.
authors.forename: Hanan A
authors.initials: HA
authors.lastname: Soliman
authors.affiliation: Zoology Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt.
authors.forename: Rasha R
authors.initials: RR
authors.lastname: Ahmed
authors.affiliation: Chemistry Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt.
authors.forename: Amal K
authors.initials: AK
authors.lastname: Khaliefa
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D018651:Cichorium intybus / Q000737:chemistry*; D003907:Dexamethasone; D005260:Female; D018649:Ocimum basilicum / Q000737:chemistry*; D010024:Osteoporosis / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D028528:Petroselinum / Q000737:chemistry*; D010936:Plant Extracts / Q000627:therapeutic use*; D051381:Rats
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D003907:Dexamethasone
keywords: Bone biomarkers; Dexamethasone; Histopathology and oxidative stress; Osteoporosis