title: De novo assembly and characterization of the Chinese three-keeled pond turtle (Mauremys reevesii) transcriptome: presence of longevity-related genes.
abstract: Mauremys reevesii (Geoemydidae) is one of the most common and widespread semi-aquatic turtles in East Asia. The unusually long lifespan of some individuals makes this turtle species a potentially useful model organism for studying the molecular basis of longevity. In this study, pooled total RNA extracted from liver, spleen and skeletal-muscle of three adult individuals were sequenced using Illumina Hiseq 2500 platform. A set of telomere-related genes were found in the transcriptome, including tert, tep1, and six shelterin complex proteins coding genes (trf1, trf2, tpp1, pot1, tin2 and rap1). These genes products protect chromosome ends from deterioration and therefore significantly contribute to turtle longevity. The transcriptome data generated in this study provides a comprehensive reference for future molecular studies in the turtle.
authors.affiliation: College of Life Science, Anhui Normal University, Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui , Wuhu, Anhui , China.
authors.forename: Huazong
authors.initials: H
authors.lastname: Yin
authors.affiliation: College of Life Science, Anhui Normal University, Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui , Wuhu, Anhui , China.
authors.forename: Liuwang
authors.initials: L
authors.lastname: Nie
authors.affiliation: College of Life Science, Anhui Normal University, Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui , Wuhu, Anhui , China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Zhao
authors.affiliation: College of Life Science, Anhui Normal University, Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui , Wuhu, Anhui , China.
authors.forename: Huaxing
authors.initials: H
authors.lastname: Zhou
authors.affiliation: College of Life Science, Anhui Normal University, Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui , Wuhu, Anhui , China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Life Science, Anhui Normal University, Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui , Wuhu, Anhui , China.
authors.forename: Xianmei
authors.initials: X
authors.lastname: Dong
authors.affiliation: College of Life Science, Anhui Normal University, Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui , Wuhu, Anhui , China.
authors.forename: Huanhuan
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Life Science, Anhui Normal University, Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui , Wuhu, Anhui , China.
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Life Science, Anhui Normal University, Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui , Wuhu, Anhui , China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Shi
authors.affiliation: College of Life Science, Anhui Normal University, Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui , Wuhu, Anhui , China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
keywords: Longevity; Mauremys reevesii; RNA-seq; Shelterin complex; Telomerase activity; Telomere; de novo assembly