title: DNA-based identification and OspC serotyping in cultures of Borrelia burgdorferi s.l. isolated from ticks collected in the Moravia (Czech Republic).
abstract: Two different genetic loci, flaB and ospC, were employed to assign genospecies and OspC phylogenetic type to 18 strains isolated from ticks collected in Pisárky, a suburban park in the city of Brno, Czech Republic. The RFLP analysis revealed three different genospecies (B. afzelii, B. garinii, and B. valaisiana). Three samples from the collection contained more than one genospecies. In the other 15 strains, nucleotide sequences of flaB and ospC were determined. The following phylogenetic analysis assigned 12 isolates to genospecies B. garinii and three to B. afzelii. These isolates were further subdivided into seven distinct ospC groups. The most related OspC types were G2, G4, and G5 (B. garinii) and A3 and A8 (B. afzelii).
authors.affiliation: Department of Animal Physiology and Immunology, Institute of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 753/5, 62500, Brno, Czech Republic. adamnorek@gmail.com.|CEITEC - Central European Institute of Technology, Masaryk University, Kamenice 753/5, 62500, Brno, Czech Republic. adamnorek@gmail.com.
authors.forename: Adam
authors.initials: A
authors.lastname: Norek
authors.affiliation: CEITEC - Central European Institute of Technology, Masaryk University, Kamenice 753/5, 62500, Brno, Czech Republic.
authors.forename: Lubomír
authors.initials: L
authors.lastname: Janda
authors.affiliation: Department of Animal Physiology and Immunology, Institute of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 753/5, 62500, Brno, Czech Republic.
authors.forename: Alena
authors.initials: A
authors.lastname: Žákovská
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D015748:Borrelia burgdorferi Group / Q000145:classification*; D018153:Czech Republic; D004269:DNA, Bacterial / Q000235:genetics; D005408:Flagellin / Q000235:genetics; D018884:Ixodes / Q000382:microbiology*; D008193:Lyme Disease; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012703:Serotyping
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; C079616:OspC protein; C064192:flaB1 protein, bacteria; D005408:Flagellin
keywords: Borrelia burgdorferi; Flagellin B; Lyme disease; OspC; Tick