title: Highly Stable Lyophilized Homogeneous Bead-Based Immunoassays for On-Site Detection of Bio Warfare Agents from Complex Matrices.
abstract: This study shows the development of dry, highly stable immunoassays for the detection of bio warfare agents in complex matrices. Thermal stability was achieved by the lyophilization of the complete, homogeneous, bead-based immunoassay in a special stabilizing buffer, resulting in a ready-to-use, simple assay, which exhibited long shelf and high-temperature endurance (up to 1 week at 100 °C). The developed methodology was successfully implemented for the preservation of time-resolved fluorescence, Alexa-fluorophores, and horse radish peroxidase-based bead assays, enabling multiplexed detection. The multiplexed assay was successfully implemented for the detection of Bacillus anthracis, botulinum B, and tularemia in complex matrices.
authors.affiliation: Department of Infectious Diseases and ‡Department of Physical Chemistry, IIBR , Ness-Ziona 74100, Israel.
authors.forename: Adva
authors.initials: A
authors.lastname: Mechaly
authors.affiliation: Department of Infectious Diseases and ‡Department of Physical Chemistry, IIBR , Ness-Ziona 74100, Israel.
authors.forename: Sharon
authors.initials: S
authors.lastname: Marx
authors.affiliation: Department of Infectious Diseases and ‡Department of Physical Chemistry, IIBR , Ness-Ziona 74100, Israel.
authors.forename: Orly
authors.initials: O
authors.lastname: Levy
authors.affiliation: Department of Infectious Diseases and ‡Department of Physical Chemistry, IIBR , Ness-Ziona 74100, Israel.
authors.forename: Shmuel
authors.initials: S
authors.lastname: Yitzhaki
authors.affiliation: Department of Infectious Diseases and ‡Department of Physical Chemistry, IIBR , Ness-Ziona 74100, Israel.
authors.forename: Morly
authors.initials: M
authors.lastname: Fisher
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry / Q000276:immunology; D001408:Bacillus anthracis / Q000276:immunology / Q000302:isolation & purification*; D054045:Biological Warfare Agents; D019274:Botulinum Toxins, Type A / Q000032:analysis*; D005604:Francisella tularensis / Q000276:immunology / Q000302:isolation & purification*; D005612:Freeze Drying; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D019095:Point-of-Care Systems
chemical_list: D055910:Antibodies, Immobilized; D054045:Biological Warfare Agents; C096323:rimabotulinumtoxinB; D019274:Botulinum Toxins, Type A