title: Light-triggered RNA release and induction of hMSC osteogenesis via photodegradable, dual-crosslinked hydrogels.
abstract: To engineer a photodegradable hydrogel system for actively controlled release of bioactive unmodified RNA at designated time points to induce hMSC osteogenesis.
  
  
  
  RNA/polyethylenimine complexes were loaded into dual-crosslinked photodegradable hydrogels to examine the capacity of UV light application to trigger their release. The ability of released RNA to drive hMSC osteogenic differentiation was also investigated.
  
  
  
  RNA release from photodegradable hydrogels was accelerated upon UV application, which was not observed in non-photodegradable hydrogels. Regardless of the presence of UV light, released siGFP exhibited high bioactivity by silencing GFP expression in HeLa cells. Importantly, siNoggin or miRNA-20a released from the hydrogels induced hMSC osteogenesis. This system provides a potentially valuable physician/patient-controlled 'on-demand' RNA delivery platform for biomedical applications.
authors.affiliation: Department of Biomedical Engineering, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA.
authors.forename: Cong Truc
authors.initials: CT
authors.lastname: Huynh
authors.affiliation: Department of Biomedical Engineering, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA.
authors.forename: Minh Khanh
authors.initials: MK
authors.lastname: Nguyen
authors.affiliation: Department of Biomedical Engineering, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA.
authors.forename: Mantas
authors.initials: M
authors.lastname: Naris
authors.affiliation: Department of Orthopaedic Surgery, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA.
authors.forename: Gulen Yesilbag
authors.initials: GY
authors.lastname: Tonga
authors.affiliation: Department of Orthopaedic Surgery, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA.
authors.forename: Vincent M
authors.initials: VM
authors.lastname: Rotello
authors.affiliation: Department of Biomedical Engineering, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA.|Department of Chemistry, University of Massachusetts, 710 North Pleasant Street, Amherst, MA 01003, USA.
authors.forename: Eben
authors.initials: E
authors.lastname: Alsberg
mesh_terms: D002454:Cell Differentiation; D002460:Cell Line; D003692:Delayed-Action Preparations / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D035683:MicroRNAs / Q000008:administration & dosage* / Q000235:genetics; D010012:Osteogenesis; D010782:Photolysis; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000235:genetics; D023822:Tissue Engineering; D014466:Ultraviolet Rays
chemical_list: D003692:Delayed-Action Preparations; D020100:Hydrogels; C534451:MIRN20a microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering
keywords: UV-controllable release; bone tissue engineering; in situ forming hydrogel; miRNA; on-demand release; photolabile hydrogels; siRNA; stem cell differentiation