title: Transforming properties of Felis catus papillomavirus type 2 E6 and E7 putative oncogenes in vitro and their transcriptional activity in feline squamous cell carcinoma in vivo.
abstract: Felis catus papillomavirus type 2 (FcaPV2) DNA is found in feline cutaneous squamous cell carcinomas (SCCs); however, its biological properties are still uncharacterized. In this study, we successfully expressed FcaPV2 E6 and E7 putative oncogenes in feline epithelial cells and demonstrated that FcaPV2 E6 binds to p53, impairing its protein level. In addition, E6 and E7 inhibited ultraviolet B (UVB)-triggered accumulation of p53, p21 and pro-apoptotic markers such as Cleaved Caspase3, Bax and Bak, suggesting a synergistic action of the virus with UV exposure in tumour pathogenesis. Furthermore, FcaPV2 E7 bound to feline pRb and impaired pRb levels, resulting in upregulation of the downstream pro-proliferative genes Cyclin A and Cdc2. Importantly, we demonstrated mRNA expression of FcaPV2 E2, E6 and E7 in feline SCC samples, strengthening the hypothesis of a causative role in the development of feline SCC.
authors.affiliation: Department of Veterinary Medicine and Animal Productions, General Pathology and Pathological Anatomy Unit, University of Naples Federico II, Via Delpino 1, 80137 Naples, Italy. Electronic address: gennaro.altamura@unina.it.
authors.forename: Gennaro
authors.initials: G
authors.lastname: Altamura
authors.affiliation: Department of Veterinary Medicine and Animal Productions, General Pathology and Pathological Anatomy Unit, University of Naples Federico II, Via Delpino 1, 80137 Naples, Italy. Electronic address: ancorteg@unina.it.
authors.forename: Annunziata
authors.initials: A
authors.lastname: Corteggio
authors.affiliation: Infections and Cancer Biology Group, International Agency for Research on Cancer, 150 Cours Albert Thomas, 69372 Lyon, France. Electronic address: PaciniL@students.iarc.fr.
authors.forename: Laura
authors.initials: L
authors.lastname: Pacini
authors.affiliation: Department of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, Via Pansini 5, 80131 Naples, Italy. Electronic address: andreaconte88@hotmail.it.
authors.forename: Andrea
authors.initials: A
authors.lastname: Conte
authors.affiliation: Department of Molecular Medicine and Medical Biotechnologies, University of Naples Federico II, Via Pansini 5, 80131 Naples, Italy. Electronic address: gmpieran@unina.it.
authors.forename: Giovanna Maria
authors.initials: GM
authors.lastname: Pierantoni
authors.affiliation: Infections and Cancer Biology Group, International Agency for Research on Cancer, 150 Cours Albert Thomas, 69372 Lyon, France. Electronic address: tommasinom@iarc.fr.
authors.forename: Massimo
authors.initials: M
authors.lastname: Tommasino
authors.affiliation: Infections and Cancer Biology Group, International Agency for Research on Cancer, 150 Cours Albert Thomas, 69372 Lyon, France. Electronic address: accardir@iarc.fr.
authors.forename: Rosita
authors.initials: R
authors.lastname: Accardi
authors.affiliation: Department of Veterinary Medicine and Animal Productions, General Pathology and Pathological Anatomy Unit, University of Naples Federico II, Via Delpino 1, 80137 Naples, Italy. Electronic address: borzacch@unina.it.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Borzacchiello
mesh_terms: D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000209:etiology*; D002352:Carrier Proteins; D002415:Cats; D002460:Cell Line; D015967:Gene Expression Regulation, Viral; D051379:Mice; D009856:Oncogene Proteins, Viral / Q000235:genetics* / Q000378:metabolism*; D027383:Papillomaviridae / Q000502:physiology*; D050725:Papillomavirus E7 Proteins / Q000235:genetics* / Q000378:metabolism*; D030361:Papillomavirus Infections / Q000662:veterinary; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics; D015398:Signal Transduction; D015533:Transcriptional Activation
chemical_list: D002352:Carrier Proteins; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012333:RNA, Messenger
keywords: E6; E7; Feline SCC; Feline oncology; Felis catus papillomavirus; Oncogenic viruses; Veterinary medicine; Veterinary virology