title: mRNA Electroporation of Dendritic Cells with WT1, Survivin, and TriMix (a Mixture of caTLR4, CD40L, and CD70).
abstract: The immune system is a crucial player in the development of cancer. Once it is in imbalance and immunosuppressive mechanisms supporting tumor growth take over control, dendritic cell immunotherapy might offer a solution to restore the balance. There are several methods to manufacture dendritic cells but none of them has yet proven to be superior to others. In this chapter, we discuss the methodology using electroporation of mRNA encoding Wilms' tumor gene 1, survivin, and TriMix (mixture of caTLR4, CD40L, and CD70) to simultaneously load and mature dendritic cells.
authors.affiliation: Laboratory of Gynecologic Oncology, Department of Oncology, KU Leuven, Leuven Cancer Institute, Leuven, Belgium. an.coosemans@gmail.com.|Department of Gynecology and Obstetrics, UZ Leuven, Herestraat 49, 3000, Leuven, Belgium. an.coosemans@gmail.com.
authors.forename: An
authors.initials: A
authors.lastname: Coosemans
authors.affiliation: Laboratory of Gynecologic Oncology, Department of Oncology, KU Leuven, Leuven Cancer Institute, Leuven, Belgium.
authors.forename: Sandra
authors.initials: S
authors.lastname: Tuyaerts
authors.affiliation: Department of Human Genetics, KU Leuven, Leuven, Belgium.
authors.forename: Kim
authors.initials: K
authors.lastname: Morias
authors.affiliation: Laboratory of Molecular and Cellular Therapy (LMCT), Vrije Universiteit Brussel, Brussels, Belgium.
authors.forename: Jurgen
authors.initials: J
authors.lastname: Corthals
authors.affiliation: Laboratory of Molecular and Cellular Therapy (LMCT), Vrije Universiteit Brussel, Brussels, Belgium.
authors.forename: Carlo
authors.initials: C
authors.lastname: Heirman
authors.affiliation: Laboratory of Molecular and Cellular Therapy (LMCT), Vrije Universiteit Brussel, Brussels, Belgium.
authors.forename: Kris
authors.initials: K
authors.lastname: Thielemans
authors.affiliation: Laboratory of Pediatric Immunology, Department of Microbiology and Immunology, KU Leuven, Leuven, Belgium.
authors.forename: Stefaan W
authors.initials: SW
authors.lastname: Van Gool
authors.affiliation: Laboratory of Gynecologic Oncology, Department of Oncology, KU Leuven, Leuven Cancer Institute, Leuven, Belgium.|Department of Gynecology and Obstetrics, UZ Leuven, Herestraat 49, 3000, Leuven, Belgium.
authors.forename: Ignace
authors.initials: I
authors.lastname: Vergote
authors.affiliation: Laboratory of Gynecologic Oncology, Department of Oncology, KU Leuven, Leuven Cancer Institute, Leuven, Belgium.|Department of Gynecology and Obstetrics, UZ Leuven, Herestraat 49, 3000, Leuven, Belgium.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Amant
mesh_terms: D000951:Antigens, Neoplasm / Q000235:genetics / Q000276:immunology*; D053282:CD27 Ligand / Q000235:genetics / Q000276:immunology; D023201:CD40 Ligand / Q000235:genetics / Q000276:immunology; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D018274:Electroporation / Q000379:methods*; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics / Q000276:immunology*; D000077022:Survivin; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000276:immunology; D025721:WT1 Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D000951:Antigens, Neoplasm; C107489:BIRC5 protein, human; D053282:CD27 Ligand; C505771:CD70 protein, human; D051034:Inhibitor of Apoptosis Proteins; D012333:RNA, Messenger; D000077022:Survivin; D051197:Toll-Like Receptor 4; D025721:WT1 Proteins; C583751:WT1 protein, human; D023201:CD40 Ligand
keywords: Dendritic cell; Electroporation; Survivin; TriMix; WT1; mRNA