title: MYC cis-Elements in PsMPT Promoter Is Involved in Chilling Response of Paeonia suffruticosa.
abstract: The MPT transports Pi to synthesize ATP. PsMPT, a chilling-induced gene, was previously reported to promote energy metabolism during bud dormancy release in tree peony. In this study, the regulatory elements of PsMPT promoter involved in chilling response were further analyzed. The PsMPT transcript was detected in different tree peony tissues and was highly expressed in the flower organs, including petal, stigma and stamen. An 1174 bp of the PsMPT promoter was isolated by TAIL-PCR, and the PsMPT promoter::GUS transgenic Arabidopsis was generated and analyzed. GUS staining and qPCR showed that the promoter was active in mainly the flower stigma and stamen. Moreover, it was found that the promoter activity was enhanced by chilling, NaCl, GA, ACC and NAA, but inhibited by ABA, mannitol and PEG. In transgenic plants harboring 421 bp of the PsMPT promoter, the GUS gene expression and the activity were significantly increased by chilling treatment. When the fragment from -421 to -408 containing a MYC cis-element was deleted, the chilling response could not be observed. Further mutation analysis confirmed that the MYC element was one of the key motifs responding to chilling in the PsMPT promoter. The present study provides useful information for further investigation of the regulatory mechanism of PsMPT during the endo-dormancy release.
authors.affiliation: College of Life Sciences, Qingdao Agricultural University, Key Lab of Plant Biotechnology in Universities of Shandong Province, Changcheng Road 700, Qingdao, China.
authors.forename: Yuxi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Qingdao Agricultural University, Key Lab of Plant Biotechnology in Universities of Shandong Province, Changcheng Road 700, Qingdao, China.
authors.forename: Tingzhao
authors.initials: T
authors.lastname: Sun
authors.affiliation: College of Life Sciences, Qingdao Agricultural University, Key Lab of Plant Biotechnology in Universities of Shandong Province, Changcheng Road 700, Qingdao, China.
authors.forename: Shaoqing
authors.initials: S
authors.lastname: Liu
authors.affiliation: College of Life Sciences, Qingdao Agricultural University, Key Lab of Plant Biotechnology in Universities of Shandong Province, Changcheng Road 700, Qingdao, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Dong
authors.affiliation: College of Life Sciences, Qingdao Agricultural University, Key Lab of Plant Biotechnology in Universities of Shandong Province, Changcheng Road 700, Qingdao, China.
authors.forename: Chunying
authors.initials: C
authors.lastname: Liu
authors.affiliation: College of Life Sciences, Qingdao Agricultural University, Key Lab of Plant Biotechnology in Universities of Shandong Province, Changcheng Road 700, Qingdao, China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Song
authors.affiliation: College of Life Sciences, Qingdao Agricultural University, Key Lab of Plant Biotechnology in Universities of Shandong Province, Changcheng Road 700, Qingdao, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Life Sciences, Qingdao Agricultural University, Key Lab of Plant Biotechnology in Universities of Shandong Province, Changcheng Road 700, Qingdao, China.
authors.forename: Shupeng
authors.initials: S
authors.lastname: Gai
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development; D001483:Base Sequence; D003080:Cold Temperature; D035264:Flowers; D018506:Gene Expression Regulation, Plant; D029596:Paeonia / Q000235:genetics* / Q000254:growth & development; D028061:Phosphate Transport Proteins / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid; D014197:Trees / Q000235:genetics / Q000254:growth & development
chemical_list: D028061:Phosphate Transport Proteins; D010940:Plant Proteins; D016271:Proto-Oncogene Proteins c-myc