title: Sirtuin1 (SIRT1) in the Acetylation of Downstream Target Proteins.
abstract: Acetylation has been shown to be an important posttranslational modification (PTM) of both histone and nonhistone proteins with particular implications in cell signaling and transcriptional regulation of gene expression. Many studies have already demonstrated that SIRT1 is able to deacetylate histones and lead to gene silencing. It can also regulate the function of tumor suppressors including FOXO proteins and p53 by deacetylation. Here, we describe three experimental approaches for studying the modulation of the acetylation status of some of the known downstream targets of SIRT1.
authors.affiliation: Department of Surgery and Cancer, Imperial College London, Hammersmith Hospital Campus, London Du Cane Road, London, W12 0NN, UK.
authors.forename: Ana R
authors.initials: AR
authors.lastname: Gomes
authors.affiliation: Department of Surgery and Cancer, Imperial College London, Hammersmith Hospital Campus, London Du Cane Road, London, W12 0NN, UK.
authors.forename: Jay Sze
authors.initials: JS
authors.lastname: Yong
authors.affiliation: Department of Surgery and Cancer, Imperial College London, Hammersmith Hospital Campus, London Du Cane Road, London, W12 0NN, UK.
authors.forename: Khai Cheng
authors.initials: KC
authors.lastname: Kiew
authors.affiliation: Department of Surgery and Cancer, Imperial College London, Hammersmith Hospital Campus, London Du Cane Road, London, W12 0NN, UK.
authors.forename: Ebru
authors.initials: E
authors.lastname: Aydin
authors.affiliation: Department of Surgery and Cancer, Imperial College London, Hammersmith Hospital Campus, London Du Cane Road, London, W12 0NN, UK.
authors.forename: Mattaka
authors.initials: M
authors.lastname: Khongkow
authors.affiliation: Department of Surgery and Cancer, Imperial College London, Hammersmith Hospital Campus, London Du Cane Road, London, W12 0NN, UK.
authors.forename: Sasiwan
authors.initials: S
authors.lastname: Laohasinnarong
authors.affiliation: Department of Surgery and Cancer, Imperial College London, Hammersmith Hospital Campus, London Du Cane Road, London, W12 0NN, UK. eric.lam@imperial.ac.uks.
authors.forename: Eric W-F
authors.initials: EW
authors.lastname: Lam
mesh_terms: D000107:Acetylation; D000818:Animals; D015153:Blotting, Western; D044127:Epigenesis, Genetic; D051858:Forkhead Transcription Factors / Q000378:metabolism*; D005786:Gene Expression Regulation; D020868:Gene Silencing; D006657:Histones / Q000378:metabolism*; D006801:Humans; D047468:Immunoprecipitation; D061986:MCF-7 Cells; D051379:Mice; D016297:Mutagenesis, Site-Directed; D056564:Sirtuin 1 / Q000235:genetics / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D051858:Forkhead Transcription Factors; D006657:Histones; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; C447939:SIRT1 protein, human; D056564:Sirtuin 1
keywords: Acetylation; Immunoprecipitation; SIRT1; Site-directed mutagenesis; Western blotting