title: Multidrug Resistance in Non-PCV13 Serotypes of Streptococcus pneumoniae in Northern Japan, 2014.
abstract: Since the implementation of routine PCV13 immunization in Japan, nonvaccine serotypes (NVTs) have been increasing among clinical isolates of Streptococcus pneumoniae. In this study, susceptibility to 18 antibiotics was tested for all the 231 isolates with NVTs, which were collected from children <16 years of age in northern Japan in 2014 (July-November). High resistance rates were observed for macrolides (>90.9%), tetracycline (91.3%), and clindamycin (75.3%), while penicillin (PEN) nonsusceptibility (PNSP; MIC ≥0.12 μg/ml) was detected in 42.9% of the pneumococci [39.4%; PEN-intermediate S. pneumoniae (PISP), 3.5%; PEN-resistant S. pneumoniae (PRSP)]. All serotype 15A isolates were PRSP (MIC, ≥2 μg/ml) or PISP, and PNSP was prevalent in also serotypes 23A (96.9%), 6C (41%), and 35B (33.3%). Overall, 42.0% of the isolates showed multidrug resistance (MDR). Sequence types (STs) determined for 20 PNSP isolates with NVTs were ST63 (15A), STs 242 or 5832 (6C), STs 338 or 5242 (23A), and ST558 (35B). All the PNSP isolates possessed tet(M), and erm(B) or mefA(A/E), and 70% of them were gPRSP having three altered genes pbp1a, pbp2x, and pbp2b. Among alterations in transpeptidase-coding region of penicillin-binding proteins (PBPs), two substitutions of T371S in the STMK motif and TSQF574-577NTGY in PBP1a were common to all PRSP isolates. The present study showed the spread of PNSP in NVTs 15A, 23A, 6C, and 35B, and the emergence of the MDR international clone Sweden15A-ST63 in northern Japan.
authors.affiliation: Department of Hygiene, Sapporo Medical University School of Medicine , Sapporo, Japan .
authors.forename: Mitsuyo
authors.initials: M
authors.lastname: Kawaguchiya
authors.affiliation: Department of Hygiene, Sapporo Medical University School of Medicine , Sapporo, Japan .
authors.forename: Noriko
authors.initials: N
authors.lastname: Urushibara
authors.affiliation: Department of Hygiene, Sapporo Medical University School of Medicine , Sapporo, Japan .
authors.forename: Nobumichi
authors.initials: N
authors.lastname: Kobayashi
mesh_terms: D000293:Adolescent; D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000276:immunology; D018432:Drug Resistance, Multiple / Q000276:immunology*; D006801:Humans; D007564:Japan; D008826:Microbial Sensitivity Tests / Q000379:methods; D046915:Penicillin-Binding Proteins / Q000276:immunology; D010406:Penicillins / Q000276:immunology; D010458:Peptidyl Transferases / Q000276:immunology; D011008:Pneumococcal Infections / Q000276:immunology / Q000382:microbiology*; D015995:Prevalence; D016415:Sequence Alignment; D065288:Serogroup; D012703:Serotyping / Q000379:methods; D013296:Streptococcus pneumoniae / Q000276:immunology*
chemical_list: D000900:Anti-Bacterial Agents; D046915:Penicillin-Binding Proteins; D010406:Penicillins; D010458:Peptidyl Transferases
keywords: Japan; multidrug resistance; non-PCV13 serotypes; penicillin binding proteins; penicillin nonsusceptible pneumococci; serotype 15A