title: A polarly localized transporter for efficient manganese uptake in rice.
abstract: Manganese is an essential metal for plant growth. A number of transporters involved in the uptake of manganese from soils, and its translocation to the shoot, have been identified in Arabidopsis and rice. However, the transporter responsible for the radial transport of manganese out of root exodermis and endodermis cells and into the root stele remains unknown. Here, we show that metal tolerance protein 9 (MTP9), a member of the cation diffusion facilitator family, is a critical player in this process in rice (Oryza sativa). We find that MTP9 is mainly expressed in roots, and that the resulting protein is localized to the plasma membrane of exo- and endodermis cells, at the proximal side of these cell layers (opposite the manganese uptake transporter Nramp5, which is found at the distal side). We demonstrate that MTP9 has manganese transport activity by expression in proteoliposomes and yeast, and show that knockout of MTP9 in rice reduces manganese uptake and its translocation to shoots. We conclude that at least in rice MTP9 is required for manganese translocation to the root stele, and thereby manganese uptake.
authors.affiliation: Faculty of Agriculture, Kochi University, 200 Otsu, Monobe, Nankoku, Kochi 783-8502, Japan.
authors.forename: Daisei
authors.initials: D
authors.lastname: Ueno
authors.affiliation: Institute of Plant Science and Resources, Okayama University, Chuo 2-20-1, Kurashiki 710-0046, Japan.
authors.forename: Akimasa
authors.initials: A
authors.lastname: Sasaki
authors.affiliation: Institute of Plant Science and Resources, Okayama University, Chuo 2-20-1, Kurashiki 710-0046, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Yamaji
authors.affiliation: Advanced Science Research Center, Okayama University, Okayama 700-8530, Japan.
authors.forename: Takaaki
authors.identifier: http://orcid.org/0000-0002-7257-7621
authors.initials: T
authors.lastname: Miyaji
authors.affiliation: Faculty of Agriculture, Kochi University, 200 Otsu, Monobe, Nankoku, Kochi 783-8502, Japan.
authors.forename: Yumi
authors.initials: Y
authors.lastname: Fujii
authors.affiliation: Faculty of Agriculture, Kochi University, 200 Otsu, Monobe, Nankoku, Kochi 783-8502, Japan.|Institute of Plant Science and Resources, Okayama University, Chuo 2-20-1, Kurashiki 710-0046, Japan.
authors.forename: Yuma
authors.initials: Y
authors.lastname: Takemoto
authors.affiliation: Department of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8530, Japan.
authors.forename: Sawako
authors.initials: S
authors.lastname: Moriyama
authors.affiliation: Institute of Plant Science and Resources, Okayama University, Chuo 2-20-1, Kurashiki 710-0046, Japan.
authors.forename: Jing
authors.initials: J
authors.lastname: Che
authors.affiliation: Advanced Science Research Center, Okayama University, Okayama 700-8530, Japan.|Department of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8530, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Moriyama
authors.affiliation: Faculty of Agriculture, Kochi University, 200 Otsu, Monobe, Nankoku, Kochi 783-8502, Japan.
authors.forename: Kozo
authors.initials: K
authors.lastname: Iwasaki
authors.affiliation: Institute of Plant Science and Resources, Okayama University, Chuo 2-20-1, Kurashiki 710-0046, Japan.
authors.forename: Jian Feng
authors.initials: JF
authors.lastname: Ma