title: Development of a highly sensitive and specific monoclonal antibody based enzyme-linked immunosorbent assay for the detection of a new β-agonist, phenylethanolamine A, in food samples.
abstract: BACKGROUND
  All β-agonists are banned as feed additives for growth promotion in animals due to toxic effects on humans after consuming the β-agonist contaminated meats. Phenylethanolamine A (PA) is a newly emerged β-agonist. Thus there is a need to develop highly sensitive and specific analytical methods for the detection of PA in food samples. In this study, the monoclonal antibody (mAb) against PA was produced by hybridoma technology and used for the development of enzyme-linked immunosorbent assay (ELISA).
  
  
  RESULTS
  The IC50values and limits of detection (LODs) of the ELISA using homogeneous combination of coating antigen/antibody for PA were 0.16 ng mL-1and 0.011 ng mL-1, respectively. The cross-reactive (CR) values of the assay with 14 structurally related β-agonists were lower than 0.59%. Swine liver and meat samples were spiked with PA at different content and analysed by ELISA. Acceptable recovery rates of 91.40-105.51% and intra-assay coefficients of variation of 1.56-9.92% (n = 3) were obtained. The ELISA for seven spiked samples was confirmed by LC-MS/MS with a high correlation coefficient of 0.9881.
  
  
  CONCLUSIONS
  The proposed mAb-based ELISA was highly sensitive and specific for PA and could be used as a quantitative/screening method for PA analysis in food samples. © 2016 Society of Chemical Industry.
authors.affiliation: The Key Lab of Health Chemistry & Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China.
authors.forename: Danni
authors.initials: D
authors.lastname: Jiang
authors.affiliation: The Key Lab of Health Chemistry & Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China.
authors.forename: Biyun
authors.initials: B
authors.lastname: Cao
authors.affiliation: College of Pharmacy Sciences, Soochow University, Suzhou 215123, China.
authors.forename: Meiyu
authors.initials: M
authors.lastname: Wang
authors.affiliation: College of Pharmacy Sciences, Soochow University, Suzhou 215123, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.affiliation: The Key Lab of Health Chemistry & Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China.
authors.forename: Kang
authors.initials: K
authors.lastname: Zhao
authors.affiliation: The Key Lab of Health Chemistry & Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Li
authors.affiliation: The Key Lab of Health Chemistry & Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China.
authors.forename: Mingxin
authors.initials: M
authors.lastname: Li
authors.affiliation: The Key Lab of Health Chemistry & Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Sun
authors.affiliation: The Key Lab of Health Chemistry & Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China.
authors.forename: Anping
authors.initials: A
authors.lastname: Deng
mesh_terms: D015078:2-Hydroxyphenethylamine / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D000318:Adrenergic beta-Agonists / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D000918:Antibody Specificity; D002681:China; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D004350:Drug Residues / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005506:Food Contamination; D005513:Food Inspection / Q000379:methods*; D006241:Haptens / Q000737:chemistry / Q000378:metabolism; D057230:Limit of Detection; D008099:Liver / Q000737:chemistry; D008460:Meat / Q000032:analysis; D015394:Molecular Structure; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D034421:Sus scrofa; D053719:Tandem Mass Spectrometry
chemical_list: C000608765:2-(4-(nitrophenyl)butan-2-ylamino)-1-(4-methoxyphenyl)ethanol; D000318:Adrenergic beta-Agonists; D000911:Antibodies, Monoclonal; D006241:Haptens; D015078:2-Hydroxyphenethylamine
keywords: enzyme-linked immunosorbent assay (ELISA); food sample; monoclonal antibody; phenylethanolamine A; β-agonist