title: Fabrication of Elasticity-Tunable Gelatinous Gel for Mesenchymal Stem Cell Culture.
abstract: Surface elasticity or stiffness of an underlying substrate may regulate cellular functions such as adhesion, proliferation, signaling, differentiation, and migration. Recent studies have reported on the development of biomaterials to control stem cell fate determination via the stiffness of the culture substrates. In this chapter, we provide a detailed protocol for fabricating elasticity-tunable gelatinous hydrogels for stem cell culture with photo-induced or thermo-induced crosslinking of well-developed styrenated gelatin (StG). We also include the detailed application of gelatinous gel for mesenchymal stem cell (MSC) culture and sample collection for transcriptional and proteomic analysis.
authors.affiliation: Laboratory of Biomedical and Biophysical Chemistry, Institute for Materials Chemistry and Engineering, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka, 819-0395, Japan.
authors.forename: Thasaneeya
authors.initials: T
authors.lastname: Kuboki
authors.affiliation: Laboratory of Biomedical and Biophysical Chemistry, Institute for Materials Chemistry and Engineering, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka, 819-0395, Japan. kidoaki@ms.ifoc.kyushu-u.ac.jp.
authors.forename: Satoru
authors.initials: S
authors.lastname: Kidoaki
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D004548:Elasticity; D005780:Gelatin / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000737:chemistry / Q000378:metabolism*; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D013499:Surface Properties
chemical_list: D020100:Hydrogels; D005780:Gelatin
keywords: Gelatinous hydrogel; MSC; Surface elasticity