title: Imaging flow cytometry for phytoplankton analysis.
abstract: This review highlights the concepts and instrumentation of imaging flow cytometry technology and in particular its use for phytoplankton analysis. Imaging flow cytometry, a hybrid technology combining speed and statistical capabilities of flow cytometry with imaging features of microscopy, is rapidly advancing as a cell imaging platform that overcomes many of the limitations of current techniques and contributed significantly to the advancement of phytoplankton analysis in recent years. This review presents the various instrumentation relevant to the field and currently used for assessment of complex phytoplankton communities' composition and abundance, size structure determination, biovolume estimation, detection of harmful algal bloom species, evaluation of viability and metabolic activity and other applications. Also we present our data on viability and metabolic assessment of Aphanizomenon sp. cyanobacteria using Imagestream X Mark II imaging cytometer. Herein, we highlight the immense potential of imaging flow cytometry for microalgal research, but also discuss limitations and future developments.
authors.affiliation: Nazarbayev University, Kazakhstan. Electronic address: veronika.dashkova@nu.edu.kz.
authors.forename: Veronika
authors.initials: V
authors.lastname: Dashkova
authors.affiliation: Nazarbayev University, Kazakhstan; Department of Hydrobiology, M.V. Lomonosov Moscow State University, Russia.
authors.forename: Dmitry
authors.initials: D
authors.lastname: Malashenkov
authors.affiliation: Bigelow Laboratory for Ocean Sciences, United States.
authors.forename: Nicole
authors.initials: N
authors.lastname: Poulton
authors.affiliation: Nazarbayev University, Kazakhstan; Department of Cell Biology and Histology and A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, Russia.
authors.forename: Ivan
authors.initials: I
authors.lastname: Vorobjev
authors.affiliation: Nazarbayev University, Kazakhstan; Program in Molecular and Cellular Medicine, BCH-Harvard Medical School, United States.
authors.forename: Natasha S
authors.initials: NS
authors.lastname: Barteneva
mesh_terms: D002734:Chlorophyll / Q000737:chemistry; D000458:Cyanobacteria / Q000378:metabolism / Q000648:ultrastructure*; D005434:Flow Cytometry / Q000379:methods*; D005452:Fluoresceins / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D057097:Harmful Algal Bloom / Q000502:physiology; D019044:Image Cytometry / Q000379:methods*; D010839:Phytoplankton / Q000378:metabolism / Q000648:ultrastructure*; D013194:Staining and Labeling / Q000379:methods
chemical_list: C110400:2',7'-dichlorodihydrofluorescein diacetate; D005452:Fluoresceins; D005456:Fluorescent Dyes; D002734:Chlorophyll
keywords: Imaging flow cytometry; Metabolic activity; Microalgae; Phytoplankton; Viability