title: MiniCORVET is a Vps8-containing early endosomal tether in Drosophila.
abstract: Yeast studies identified two heterohexameric tethering complexes, which consist of 4 shared (Vps11, Vps16, Vps18 and Vps33) and 2 specific subunits: Vps3 and Vps8 (CORVET) versus Vps39 and Vps41 (HOPS). CORVET is an early and HOPS is a late endosomal tether. The function of HOPS is well known in animal cells, while CORVET is poorly characterized. Here we show that Drosophila Vps8 is highly expressed in hemocytes and nephrocytes, and localizes to early endosomes despite the lack of a clear Vps3 homolog. We find that Vps8 forms a complex and acts together with Vps16A, Dor/Vps18 and Car/Vps33A, and loss of any of these proteins leads to fragmentation of endosomes. Surprisingly, Vps11 deletion causes enlargement of endosomes, similar to loss of the HOPS-specific subunits Vps39 and Lt/Vps41. We thus identify a 4 subunit-containing miniCORVET complex as an unconventional early endosomal tether in Drosophila.
authors.affiliation: Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd University, Budapest, Hungary.
authors.forename: Péter
authors.identifier: 0000-0001-7374-667X
authors.initials: P
authors.lastname: Lőrincz
authors.affiliation: Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd University, Budapest, Hungary.
authors.forename: Zsolt
authors.identifier: 0000-0003-1900-3167
authors.initials: Z
authors.lastname: Lakatos
authors.affiliation: Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd University, Budapest, Hungary.
authors.forename: Ágnes
authors.initials: Á
authors.lastname: Varga
authors.affiliation: Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary.
authors.forename: Tamás
authors.identifier: 0000-0001-8142-3221
authors.initials: T
authors.lastname: Maruzs
authors.affiliation: Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary.
authors.forename: Zsófia
authors.identifier: 0000-0001-7909-4895
authors.initials: Z
authors.lastname: Simon-Vecsei
authors.affiliation: Laboratory of Proteomics Research, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary.
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Darula
authors.affiliation: Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd University, Budapest, Hungary.
authors.forename: Péter
authors.initials: P
authors.lastname: Benkő
authors.affiliation: Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary.
authors.forename: Gábor
authors.initials: G
authors.lastname: Csordás
authors.affiliation: Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd University, Budapest, Hungary.
authors.forename: Mónika
authors.initials: M
authors.lastname: Lippai
authors.affiliation: Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary.
authors.forename: István
authors.initials: I
authors.lastname: Andó
authors.affiliation: Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd University, Budapest, Hungary.
authors.forename: Krisztina
authors.initials: K
authors.lastname: Hegedűs
authors.affiliation: Laboratory of Proteomics Research, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary.
authors.forename: Katalin F
authors.initials: KF
authors.lastname: Medzihradszky
authors.affiliation: Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd University, Budapest, Hungary.
authors.forename: Szabolcs
authors.initials: S
authors.lastname: Takáts
authors.affiliation: Department of Anatomy, Cell and Developmental Biology, Eötvös Loránd University, Budapest, Hungary.|Institute of Genetics, Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary.
authors.forename: Gábor
authors.identifier: 0000-0001-8548-8874
authors.initials: G
authors.lastname: Juhász
mesh_terms: D000818:Animals; D029721:Drosophila Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D011992:Endosomes / Q000378:metabolism*; D005786:Gene Expression Regulation; D006434:Hemocytes / Q000378:metabolism; D046912:Multiprotein Complexes / Q000235:genetics / Q000378:metabolism*; D009399:Nephrons / Q000378:metabolism; D020798:Two-Hybrid System Techniques; D033921:Vesicular Transport Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D029721:Drosophila Proteins; D046912:Multiprotein Complexes; D033921:Vesicular Transport Proteins; C000624420:Vps8 protein, Drosophila
keywords: CORVET; D. melanogaster; HOPS; Vps8; cell biology; endocytosis; fusion; tether