title: Protective effects of 1α,25-Dihydroxyvitamin D3 on cultured neural cells exposed to catalytic iron.
abstract: Recent studies have postulated a role for vitamin D and its receptor on cerebral function, and anti-inflammatory, immunomodulatory and neuroprotective effects have been described; vitamin D can inhibit proinflammatory cytokines and nitric oxide synthesis during various neurodegenerative insults, and may be considered as a potential drug for the treatment of these disorders. In addition, iron is crucial for neuronal development and neurotransmitter production in the brain, but its accumulation as catalytic form (Fe(3+)) impairs brain function and causes the dysregulation of iron metabolism leading to tissue damage due to the formation of toxic free radicals (ROS). This research was planned to study the role of vitamin D to prevent iron damage in neuroblastoma BE(2)M17 cells. Mechanisms involved in neurodegeneration, including cell viability, ROS production, and the most common intracellular pathways were studied. Pretreatment with calcitriol (the active form of vitamin D) reduced cellular injury induced by exposure to catalytic iron.
authors.affiliation: Laboratory of Physiology, Department of Translational Medicine, UPO - University of Eastern Piedmont, Novara, Italy francesca.uberti@med.uniupo.it.
authors.forename: Francesca
authors.initials: F
authors.lastname: Uberti
authors.affiliation: Laboratory of Physiology, Department of Translational Medicine, UPO - University of Eastern Piedmont, Novara, Italy.
authors.forename: Vera
authors.initials: V
authors.lastname: Morsanuto
authors.affiliation: Laboratory of Physiology, Department of Translational Medicine, UPO - University of Eastern Piedmont, Novara, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Bardelli
authors.affiliation: Laboratory of Physiology, Department of Translational Medicine, UPO - University of Eastern Piedmont, Novara, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Molinari
mesh_terms: D002117:Calcitriol / Q000494:pharmacology* / Q000627:therapeutic use; D045744:Cell Line, Tumor; D006801:Humans; D007501:Iron / Q000494:pharmacology*; D009410:Nerve Degeneration / Q000139:chemically induced / Q000517:prevention & control*; D009474:Neurons / Q000187:drug effects* / Q000473:pathology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D018167:Receptors, Calcitriol / Q000378:metabolism; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D018696:Neuroprotective Agents; D017382:Reactive Oxygen Species; D018167:Receptors, Calcitriol; C118756:dihydroxy-vitamin D3; D014807:Vitamin D; D007501:Iron; D002117:Calcitriol
keywords: Active vitamin D; catalytic iron; human neuronal cells