title: Immunotherapy with iTreg and nTreg Cells in a Murine Model of Inflammatory Bowel Disease.
abstract: Regulatory T (Treg) cells that express the transcription factor Foxp3 are essential for maintaining tolerance at mucosal interfaces, where they act by controlling inflammation and promoting epithelial cell homeostasis. There are two major regulatory T-cell subsets, "natural" CD4(+) Treg (nTreg) cells that develop in the thymus and "induced" Treg (iTreg) cells that develop from conventional CD4(+) T (Tconv) cells in the periphery. Dysregulated Treg cell responses are associated with autoimmune diseases, including inflammatory bowel disease (IBD) and arthritis. Adoptive transfer of Treg cells can modulate innate and adaptive immune responses and cure disease in animal models, which has generated considerable interest in using Treg cells to treat human autoimmune disease, prevent rejection of transplanted organs, and to control graft-versus-host disease following hematopoietic stem cell transplantation. Herein, we describe our modifications of a treatment model of T-cell transfer colitis designed to allow mechanistic investigation of the two major Treg cell subsets and to compare their specific roles in mucosal tolerance.
authors.affiliation: Section of Rheumatology, Department of Pediatrics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI, 53226, USA.
authors.forename: Dipica
authors.initials: D
authors.lastname: Haribhai
authors.affiliation: Division of Immunology, Children's Hospital Boston, Boston, MA, 02115, USA.
authors.forename: Talal A
authors.initials: TA
authors.lastname: Chatila
authors.affiliation: Section of Rheumatology, Department of Pediatrics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI, 53226, USA. cwilliam@mcw.edu.
authors.forename: Calvin B
authors.initials: CB
authors.lastname: Williams
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000637:transplantation; D002469:Cell Separation; D004195:Disease Models, Animal; D051858:Forkhead Transcription Factors / Q000378:metabolism; D006801:Humans; D007167:Immunotherapy; D015212:Inflammatory Bowel Diseases / Q000276:immunology / Q000378:metabolism / Q000628:therapy*; D051379:Mice; D050378:T-Lymphocytes, Regulatory / Q000166:cytology / Q000276:immunology / Q000637:transplantation*
chemical_list: D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse
keywords: Adoptive T-cell transfer; Foxp3; Immunotherapy; Induced regulatory T cells; Inflammatory bowel disease; Natural regulatory T cells