title: Biological removal of pharmaceutical compounds using white-rot fungi with concomitant FAME production of the residual biomass.
abstract: The efficiency of two white-rot fungi (WRF), Trametes versicolor and Ganoderma lucidum, to eliminate thirteen pharmaceutical pollutants with concomitant biodiesel production from the accumulating lipid content after treatment, was examined. The removal efficiency was studied using both individual and combined strains. The results of individual and combined strains showed a total removal (100%) of diclofenac (DCF), gemfibrozil (GFZ), ibuprofen (IBP), progesterone (PGT) and ranitidine (RNT). Lower removals were achieved for 4-acetamidoantipyrin (AAA), clofibric acid (ACF), atenolol (ATN), caffeine (CFN), carbamazepine (CZP), hydrochlorothiazide (HCT), sulfamethoxazole (SMX) and sulpiride (SPD), although the combination of both strains enhanced the system's efficiency, with removals ranging from 15 to 41%. This increase of the removal efficiency when combining both strains was attributed to the interactions developed between them (i.e., competition). Results from enzymatic and cytochrome P450 examination suggested that both extracellular (laccase, MnP, LiP) and intracellular oxidation mechanisms participate in the biological removal of pharmaceuticals. On the other hand, the "green" potential of the fungal sludge generated during the biological removal process was assessed for biodiesel production by means of one-step direct (in-situ) transformation. This process consists of the simultaneous extraction and conversion of lipids contained in the sludge by catalytic esterification/transesterification using a robust acid heterogeneous Zr-SBA-15 catalyst. This catalytic system provided conversions close to 80% of the saponifiable fraction (including free fatty acids and glycerides) in the presence of high amount of impurities. The overall weight FAME yield, based on the initial dried mass, was close to 30% for both strains.
authors.affiliation: School of Experimental Sciences and Technology (ESCET), Rey Juan Carlos University, c/Tulipán s/n. Móstoles, 28933, Madrid, Spain. Electronic address: ioanna.vasiliadou@urjc.es.
authors.forename: I A
authors.initials: IA
authors.lastname: Vasiliadou
authors.affiliation: School of Experimental Sciences and Technology (ESCET), Rey Juan Carlos University, c/Tulipán s/n. Móstoles, 28933, Madrid, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Sánchez-Vázquez
authors.affiliation: School of Experimental Sciences and Technology (ESCET), Rey Juan Carlos University, c/Tulipán s/n. Móstoles, 28933, Madrid, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Molina
authors.affiliation: School of Experimental Sciences and Technology (ESCET), Rey Juan Carlos University, c/Tulipán s/n. Móstoles, 28933, Madrid, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Martínez
authors.affiliation: School of Experimental Sciences and Technology (ESCET), Rey Juan Carlos University, c/Tulipán s/n. Móstoles, 28933, Madrid, Spain.
authors.forename: J A
authors.initials: JA
authors.lastname: Melero
authors.affiliation: School of Experimental Sciences and Technology (ESCET), Rey Juan Carlos University, c/Tulipán s/n. Móstoles, 28933, Madrid, Spain.
authors.forename: L F
authors.initials: LF
authors.lastname: Bautista
authors.affiliation: School of Experimental Sciences and Technology (ESCET), Rey Juan Carlos University, c/Tulipán s/n. Móstoles, 28933, Madrid, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Iglesias
authors.affiliation: School of Experimental Sciences and Technology (ESCET), Rey Juan Carlos University, c/Tulipán s/n. Móstoles, 28933, Madrid, Spain.
authors.forename: G
authors.initials: G
authors.lastname: Morales
mesh_terms: D056804:Biofuels / Q000382:microbiology*; D018533:Biomass; D005658:Fungi / Q000378:metabolism*; D006801:Humans; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000378:metabolism*; D018508:Water Purification / Q000379:methods
chemical_list: D056804:Biofuels; D004364:Pharmaceutical Preparations; D014874:Water Pollutants, Chemical
keywords: Ganoderma lucidum; Heterogeneous catalyst; In-situ transesterification treatment; PPCPs; Trametes versicolor; Zr-SBA-15