title: The Impact of Serum Amyloid P-Component on Gene Expression in RAW264.7 Mouse Macrophages.
abstract: Serum amyloid P-component (SAP) contributes to host defense and prevents fibrosis. Macrophages are the most abundant inflammatory cell type in atherosclerotic plaques. In the present study, using (3)H-cholesterol-labeled counting radioactivity assay, we demonstrated that the apoAI-mediated cholesterol efflux in RAW264.7 macrophages was increased by SAP treatment in a time- and dose-dependent manner. We analyzed global gene expression changes upon SAP treatment using RNA sequencing. As a result, a total of 175 differentially expressed genes were identified, of which 134 genes were downregulated and 41 genes were upregulated in SAP treated cells compared to control cells. Quantitative RT-PCR analysis confirmed decreased expression of 5 genes and an increase in expression of 1 gene upon SAP treatment. Gene ontology analysis showed that genes involved in response to stimulus were significantly enriched in differentially expressed genes. Beyond protein-coding genes, we also identified 8 differentially expressed long noncoding RNAs. Our study may provide new insights into mechanisms underlying the functional role of SAP in macrophages.
authors.affiliation: Division of Cardiology, Huiqiao Medical Center, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Xi
authors.affiliation: Division of Cardiology, Huiqiao Medical Center, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China.
authors.forename: Jinzhen
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Division of Cardiology, Huiqiao Medical Center, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China.
authors.forename: Jichen
authors.initials: J
authors.lastname: Liu
authors.affiliation: Division of Cardiology, Huiqiao Medical Center, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China.
authors.forename: Haowei
authors.initials: H
authors.lastname: Xiong
authors.affiliation: Division of Cardiology, Huiqiao Medical Center, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China.
authors.forename: Wenshuai
authors.initials: W
authors.lastname: He
authors.affiliation: Division of Cardiology, Huiqiao Medical Center, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Hu
authors.affiliation: Division of Cardiology, Huiqiao Medical Center, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China.
authors.forename: Wenyan
authors.initials: W
authors.lastname: Lai
authors.affiliation: Division of Cardiology, Huiqiao Medical Center, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Guo
mesh_terms: D000818:Animals; D016632:Apolipoprotein A-I / Q000276:immunology*; D002784:Cholesterol / Q000276:immunology*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D000067996:RAW 264.7 Cells; D000683:Serum Amyloid P-Component / Q000008:administration & dosage* / Q000276:immunology*
chemical_list: D016632:Apolipoprotein A-I; D000683:Serum Amyloid P-Component; D002784:Cholesterol