title: The Pro12Ala polymorphism in the PPAR-γ2 gene is not associated to obesity and type 2 diabetes mellitus in a Cameroonian population.
abstract: BACKGROUND
  Peroxisome proliferator-activated receptor gamma 2 (PPAR-γ2) is a transcription factor with a key role in adipocyte differentiation, lipid storage and glucose homeostasis. The Ala allele of the common Pro12Ala polymorphism in the isoform PPAR-γ2 is at the center of many controversies because in some populations, it has been observed to be associated with T2DM or obesity but, not in others. The aim of this study was to investigate the association of Pro12Ala polymorphism in the PPAR-γ2 gene with susceptibility to obesity or T2DM in a Cameroonian population.
  
  
  METHODS
  This case-control study included 62 obese, 60 T2DM patients and 120 controls (60 non obese and 60 patients without T2DM), all unrelated and of Cameroonian origin. PPAR-γ2 was examined by genotyping for Pro12Ala using the Restriction Fragment Length Polymorphism - Polymerase Chain Reaction (PCR - RFLP).
  
  
  RESULTS
  A portion of the 270 base pair bands of the PPAR-γ2 gene was successfully amplified. The Ala12 variant was totally absent from the study population, all participants being homozygote Pro/Pro.
  
  
  CONCLUSIONS
  PPAR-γ2 Pro12Ala gene polymorphism may not be associated with obesity and T2DM. These results suggest that, PPAR-γ2 is unlikely a major gene for obesity or T2DM in the study population.
authors.affiliation: Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon.|Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon.
authors.forename: Edith Pascale M
authors.initials: EPM
authors.lastname: Mato
authors.affiliation: Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon.|Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon.
authors.forename: Priscille Eunice
authors.initials: PE
authors.lastname: Pokam-Fosso
authors.affiliation: Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon.|Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon.
authors.forename: Barbara
authors.initials: B
authors.lastname: Atogho-Tiedeu
authors.affiliation: Department of Medicine, Groote Schuur Hospital and University of Cape Town, Cape Town, South Africa.|Medical Diagnostic Center, Yaoundé, Cameroon.
authors.forename: Jean Jacques N
authors.initials: JJN
authors.lastname: Noubiap
authors.affiliation: Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon.|Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon.
authors.forename: Marie-Solange
authors.initials: MS
authors.lastname: Evehe
authors.affiliation: Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon.|Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon.
authors.forename: Rosine
authors.initials: R
authors.lastname: Djokam-Dadjeu
authors.affiliation: Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon.|Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon.
authors.forename: Olivier Sontsa
authors.initials: OS
authors.lastname: Donfack
authors.affiliation: Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon.|Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon.
authors.forename: Elvis Ndonwi
authors.initials: EN
authors.lastname: Ngwa
authors.affiliation: Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon.|Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon.
authors.forename: Magellan
authors.initials: M
authors.lastname: Guewo-Fokeng
authors.affiliation: Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon.|Laboratory for Public Health Research and Biotechnologies, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon.
authors.forename: Wilfred F
authors.initials: WF
authors.lastname: Mbacham
authors.affiliation: Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon.|Department of Internal Medicine and Specialties, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon.|National Obesity Center, Yaoundé Central Hospital and Faculty of Medicine and Biomedical Sciences, University of Yaoundé 1, Yaoundé, Cameroon.
authors.forename: Eugene
authors.initials: E
authors.lastname: Sobngwi
authors.affiliation: Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaoundé I, Yaoundé, Cameroon.|Department of Internal Medicine and Specialties, Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, Yaoundé, Cameroon.|National Obesity Center, Yaoundé Central Hospital and Faculty of Medicine and Biomedical Sciences, University of Yaoundé 1, Yaoundé, Cameroon.
authors.forename: Jean Claude
authors.initials: JC
authors.lastname: Mbanya
keywords: Genetic association; Obesity; PPAR gamma2; PPAR-γ2; Pro12Ala; Type 2 diabetes mellitus