title: Miniature fiber-optic multiphoton microscopy system using frequency-doubled femtosecond Er-doped fiber laser.
abstract: We report on a miniature fiber-optic multiphoton microscopy (MPM) system based on a frequency-doubled femtosecond Er-doped fiber laser. The femtosecond pulses from the laser source are delivered to the miniature fiber-optic probe at 1.58 µm wavelength, where a standard single mode fiber is used for delivery without the need of free-space dispersion compensation components. The beam is frequency-doubled inside the probe by a periodically poled MgO:LiNbO3 crystal. Frequency-doubled pulses at 786 nm with a maximum power of 80 mW and a pulsewidth of 150 fs are obtained and applied to excite intrinsic signals from tissues. A MEMS scanner, a miniature objective, and a multimode collection fiber are further used to make the probe compact. The miniature fiber-optic MPM system is highly portable and robust. Ex vivo multiphoton imaging of mammalian skins demonstrates the capability of the system in imaging biological tissues. The results show that the miniature fiber-optic MPM system using frequency-doubled femtosecond fiber laser can potentially bring the MPM imaging for clinical applications.
authors.affiliation: Department of Electrical and Computer Engineering, University of British Columbia, Vancouver, V6T 1Z4, Canada.
authors.forename: Lin
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Physics & Astronomy, University of British Columbia, Vancouver, V6T 1Z4, Canada.
authors.forename: Arthur K
authors.initials: AK
authors.lastname: Mills
authors.affiliation: Department of Electrical and Computer Engineering, University of British Columbia, Vancouver, V6T 1Z4, Canada.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Physics & Astronomy, University of British Columbia, Vancouver, V6T 1Z4, Canada.
authors.forename: David J
authors.initials: DJ
authors.lastname: Jones
authors.affiliation: Department of Electrical and Computer Engineering, University of British Columbia, Vancouver, V6T 1Z4, Canada.
authors.forename: Shuo
authors.initials: S
authors.lastname: Tang
keywords: (170.2150) Endoscopic imaging; (190.4180) Multiphoton processes