title: Absence of an association of human polyomavirus and papillomavirus infection with lung cancer in China: a nested case-control study.
abstract: Studies of human polyomavirus (HPyV) infection and lung cancer are limited and those regarding the association of human papillomavirus (HPV) infection and lung cancer have produced inconsistent results.
  
  
  
  We conducted a nested case-control study to assess the association between incident lung cancer of various histologies and evidence of prior infection with HPyVs and HPVs. We selected serum from 183 cases and 217 frequency matched controls from the Yunnan Tin Miner's Cohort study, which was designed to identify biomarkers for early detection of lung cancer. Using multiplex liquid bead microarray (LBMA) antibody assays, we tested for antibodies to the VP1 structural protein and small T antigen (ST-Ag) of Merkel cell, KI, and WU HPyVs. We also tested for antibodies against HPV L1 structural proteins (high-risk types 16, 18, 31, 33, 52, and 58 and low-risk types 6 and 11) and E6 and E7 oncoproteins (high risk types 16 and 18). Measures of antibody reactivity were log transformed and analyzed using logistic regression.
  
  
  
  We found no association between KIV, WUV, and MCV antibody levels and incident lung cancer (P-corrected for multiple comparisons >0.10 for all trend tests). We also found no association with HPV-16, 18, 31, 33, 52, and 58 seropositivity (P-corrected for multiple comparisons >0.05 for all).
  
  
  
  Future studies of infectious etiologies of lung cancer should look beyond HPyVs and HPVs as candidate infectious agents.
authors.affiliation: Department of Epidemiology, School of Public Health, University of Washington, Seattle, WA, USA. dvc2@uw.edu.|Fred Hutchinson Cancer Research Center, Seattle, WA, USA. dvc2@uw.edu.|Institute for Health Metrics and Evaluation, University of Washington, 2301 5th Avenue, Suite 600, Seattle, WA, 98121, USA. dvc2@uw.edu.
authors.forename: Danny V
authors.initials: DV
authors.lastname: Colombara
authors.affiliation: Department of Epidemiology, School of Public Health, University of Washington, Seattle, WA, USA.
authors.forename: Lisa E
authors.initials: LE
authors.lastname: Manhart
authors.affiliation: Fred Hutchinson Cancer Research Center, Seattle, WA, USA.
authors.forename: Joseph J
authors.initials: JJ
authors.lastname: Carter
authors.affiliation: Department of Epidemiology, School of Public Health, University of Washington, Seattle, WA, USA.
authors.forename: Stephen E
authors.initials: SE
authors.lastname: Hawes
authors.affiliation: Department of Epidemiology, School of Public Health, University of Washington, Seattle, WA, USA.|Fred Hutchinson Cancer Research Center, Seattle, WA, USA.
authors.forename: Noel S
authors.initials: NS
authors.lastname: Weiss
authors.affiliation: Fred Hutchinson Cancer Research Center, Seattle, WA, USA.|Department of Biostatistics, School of Public Health, University of Washington, Seattle, WA, USA.
authors.forename: James P
authors.initials: JP
authors.lastname: Hughes
authors.affiliation: Department of Cancer Epidemiology, Cancer Institute, Chinese Academy of Medical Sciences, Beijing, China.
authors.forename: You-Lin
authors.initials: YL
authors.lastname: Qiao
authors.affiliation: Genetic Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, USA.
authors.forename: Philip R
authors.initials: PR
authors.lastname: Taylor
authors.affiliation: Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, NC, USA.
authors.forename: Jennifer S
authors.initials: JS
authors.lastname: Smith
authors.affiliation: Fred Hutchinson Cancer Research Center, Seattle, WA, USA.
authors.forename: Denise A
authors.initials: DA
authors.lastname: Galloway
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D005260:Female; D006801:Humans; D015994:Incidence; D008175:Lung Neoplasms / Q000453:epidemiology / Q000821:virology*; D008297:Male; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000150:complications / Q000453:epidemiology*; D027601:Polyomavirus Infections / Q000150:complications / Q000453:epidemiology*
keywords: Human papillomavirus; Human polyomavirus; KI polyomavirus; Lung cancer; Merkel cell polyomavirus; WU polyomavirus