title: Selection of Natural and Base-Modified DNA Aptamers for a Camptothecin Derivative.
abstract: Nucleic acid aptamers for small molecules are currently being developed and have a potential role in diverse applications including biosensing, diagnostics, and therapeutics involving low-molecular-weight biomarkers and drugs. To enhance and broaden their functions through chemical modification, systematic evolution of ligands by exponential enrichment (SELEX) selection has been attempted with modified DNA/RNA libraries. Recently, we demonstrated the superior efficacy of base modification for affinity enhancement and the usefulness of unnatural nucleic acid libraries for development of small-molecule aptamers. In this unit, we describe construction of a modified DNA library that includes (E)-5-(2-(N-(2-(N(6) -adeninyl)ethyl))carbamylvinyl)uracil bases and acquisition of high-affinity camptothecin-binding DNA aptamers, in addition to those of the corresponding natural DNA library and aptamers, using the SELEX method. © 2016 by John Wiley & Sons, Inc.
authors.affiliation: Graduate School of Science and Technology, Gunma University, Kiryu, Gunma, Japan.
authors.forename: Hiroto
authors.initials: H
authors.lastname: Fujita
authors.affiliation: Graduate School of Science and Technology, Gunma University, Kiryu, Gunma, Japan.
authors.forename: Masayasu
authors.initials: M
authors.lastname: Kuwahara
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D002166:Camptothecin / Q000737:chemistry*; D019143:Evolution, Molecular; D015723:Gene Library; D008024:Ligands; D054852:Small Molecule Libraries / Q000737:chemistry*; D014498:Uracil / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: C585546:(E)-5-(2-(N-(2-(N6-adeninyl)ethyl))carbamylvinyl)-uracil; D052157:Aptamers, Nucleotide; D008024:Ligands; D054852:Small Molecule Libraries; D014498:Uracil; D002166:Camptothecin
keywords: modified DNA; nucleic acid aptamers; selex; small molecules