title: Intracellular calcium signaling regulates glomerular filtration barrier permeability: the role of the PKGIα-dependent pathway.
abstract: Podocytes are dynamic polarized cells that lie on the surface of glomerular capillaries and comprise an essential component of the glomerular filtration barrier. Insulin provoked a sustained, approximately 70%, increase in intracellular calcium concentration in podocytes. RT-PCR revealed the presence of mRNA encoding sarco/endoplasmic reticulum calcium ATPase isoforms 1-3, and plasma membrane Ca(2+) pump (PMCA) isoforms 1,3,4; mRNA levels were depressed by the addition of insulin. Inhibitors of PMCA, and the Na(+) -Ca(2+) exchanger, increased podocyte permeability to albumin, induced dimerization of protein kinase G type I alpha (PKGIα), and activation of PKGIα-dependent signaling. These data suggest the involvement of calcium and PKGIα signaling in insulin-enhanced filtration barrier permeability in podocytes.
authors.affiliation: Laboratory of Molecular and Cellular Nephrology, Mossakowski Medical Research Centre Polish Academy of Sciences, Gdańsk, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Piwkowska
authors.affiliation: Laboratory of Molecular and Cellular Nephrology, Mossakowski Medical Research Centre Polish Academy of Sciences, Gdańsk, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Rogacka
authors.affiliation: Laboratory of Molecular and Cellular Nephrology, Mossakowski Medical Research Centre Polish Academy of Sciences, Gdańsk, Poland.
authors.forename: Irena
authors.initials: I
authors.lastname: Audzeyenka
authors.affiliation: Department of Medicine, Cardio-Renal Physiology and Medicine, University of Alabama at Birmingham, AL, USA.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Kasztan
authors.affiliation: Laboratory of Molecular and Cellular Nephrology, Mossakowski Medical Research Centre Polish Academy of Sciences, Gdańsk, Poland.
authors.forename: Stefan
authors.initials: S
authors.lastname: Angielski
authors.affiliation: Laboratory of Molecular and Cellular Nephrology, Mossakowski Medical Research Centre Polish Academy of Sciences, Gdańsk, Poland.|Department of Clinical Chemistry, Medical University of Gdańsk, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Jankowski
mesh_terms: D000818:Animals; D020013:Calcium Signaling / Q000502:physiology*; D062965:Cyclic GMP-Dependent Protein Kinase Type I / Q000235:genetics / Q000378:metabolism*; D005919:Glomerular Filtration Rate / Q000502:physiology*; D053499:Plasma Membrane Calcium-Transporting ATPases / Q000235:genetics / Q000378:metabolism; D050199:Podocytes / Q000166:cytology / Q000378:metabolism*; D055503:Protein Multimerization / Q000502:physiology*; D051381:Rats
chemical_list: D062965:Cyclic GMP-Dependent Protein Kinase Type I; D053499:Plasma Membrane Calcium-Transporting ATPases
keywords: Insulin; calcium; contraction apparatus; filtration barrier permeability; podocyte