title: Physical interaction between peroxisomes and chloroplasts elucidated by in situ laser analysis.
abstract: Life on earth relies upon photosynthesis, which consumes carbon dioxide and generates oxygen and carbohydrates. Photosynthesis is sustained by a dynamic environment within the plant cell involving numerous organelles with cytoplasmic streaming. Physiological studies of chloroplasts, mitochondria and peroxisomes show that these organelles actively communicate during photorespiration, a process by which by-products produced by photosynthesis are salvaged. Nevertheless, the mechanisms enabling efficient exchange of metabolites have not been clearly defined. We found that peroxisomes along chloroplasts changed shape from spherical to elliptical and their interaction area increased during photorespiration. We applied a recent femtosecond laser technology to analyse adhesion between the organelles inside palisade mesophyll cells of Arabidopsis leaves and succeeded in estimating their physical interactions under different environmental conditions. This is the first application of this estimation method within living cells. Our findings suggest that photosynthetic-dependent interactions play a critical role in ensuring efficient metabolite flow during photorespiration.
authors.affiliation: Department of Cell Biology, National Institute for Basic Biology, Okazaki 444-8585, Aichi, Japan.
authors.forename: Kazusato
authors.initials: K
authors.lastname: Oikawa
authors.affiliation: Hayama Center for Advanced Studies, SOKENDAI (The Graduate University for Advanced Studies), Hayama 240-0193, Kanagawa, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Matsunaga
authors.affiliation: Department of Cell Biology, National Institute for Basic Biology, Okazaki 444-8585, Aichi, Japan.|Department of Basic Biology, School of Life Science, SOKENDAI (The Graduate University for Advanced Studies), Okazaki 444-8585, Aichi, Japan.
authors.forename: Shoji
authors.initials: S
authors.lastname: Mano
authors.affiliation: Department of Cell Biology, National Institute for Basic Biology, Okazaki 444-8585, Aichi, Japan.
authors.forename: Maki
authors.initials: M
authors.lastname: Kondo
authors.affiliation: Department of Cell Biology, National Institute for Basic Biology, Okazaki 444-8585, Aichi, Japan.|Department of Basic Biology, School of Life Science, SOKENDAI (The Graduate University for Advanced Studies), Okazaki 444-8585, Aichi, Japan.
authors.forename: Kenji
authors.identifier: http://orcid.org/0000-0003-4872-3729
authors.initials: K
authors.lastname: Yamada
authors.affiliation: Department of Cell Biology, National Institute for Basic Biology, Okazaki 444-8585, Aichi, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Hayashi
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Ibaraki, Japan.
authors.forename: Takatoshi
authors.initials: T
authors.lastname: Kagawa
authors.affiliation: Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University.
authors.forename: Akeo
authors.initials: A
authors.lastname: Kadota
authors.affiliation: Institute of Plant Science and Resources, Okayama University, Kurashiki 710-0046, Okayama, Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Sakamoto
authors.affiliation: Okazaki Large Spectrograph, National Institute for Basic Biology, Okazaki 444-8585, Aichi, Japan.
authors.forename: Shoichi
authors.initials: S
authors.lastname: Higashi
authors.affiliation: Hayama Center for Advanced Studies, SOKENDAI (The Graduate University for Advanced Studies), Hayama 240-0193, Kanagawa, Japan.|Okazaki Large Spectrograph, National Institute for Basic Biology, Okazaki 444-8585, Aichi, Japan.
authors.forename: Masakatsu
authors.initials: M
authors.lastname: Watanabe
authors.affiliation: Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University, Niigata 950-2181, Niigata, Japan.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Mitsui
authors.affiliation: Graduate School of Materials Science, Nara Institute of Science and Technology, Ikoma 630-0192, Nara, Japan.
authors.forename: Akinori
authors.initials: A
authors.lastname: Shigemasa
authors.affiliation: Graduate School of Materials Science, Nara Institute of Science and Technology, Ikoma 630-0192, Nara, Japan.
authors.forename: Takanori
authors.initials: T
authors.lastname: Iino
authors.affiliation: Graduate School of Materials Science, Nara Institute of Science and Technology, Ikoma 630-0192, Nara, Japan.
authors.forename: Yoichiroh
authors.initials: Y
authors.lastname: Hosokawa
authors.affiliation: Department of Cell Biology, National Institute for Basic Biology, Okazaki 444-8585, Aichi, Japan.|Department of Basic Biology, School of Life Science, SOKENDAI (The Graduate University for Advanced Studies), Okazaki 444-8585, Aichi, Japan.
authors.forename: Mikio
authors.initials: M
authors.lastname: Nishimura
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism; D017360:Arabidopsis / Q000166:cytology* / Q000502:physiology; D002736:Chloroplasts / Q000378:metabolism*; D008027:Light; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000378:metabolism; D020675:Peroxisomes / Q000378:metabolism*; D010788:Photosynthesis / Q000502:physiology; D059828:Plant Cells; D018515:Plant Leaves / Q000166:cytology; D030821:Plants, Genetically Modified