title: Calcium phosphate nanoparticles-based systems for siRNA delivery.
abstract: Despite the enormous therapeutic potential of siRNA as a treatment strategy, the delivery is still a problem due to unfavorable biodistribution profiles and poor intracellular bioavailability. Calcium phosphate (CaP) co-precipitate has been used for nearly 40 years for in vitro transfection due to its non-toxic nature and simplicity of preparation. The surface charge of CaP will be tuned into positive by surface modification, which is important for siRNA loading and crossing cell membrane without enzymatic degradation. The new siRNA carrier system will also promote the siRNA escape from lysosome to achieve siRNA sustained delivery and high-efficiency silence. In this review, we focus on the current research activity in the development of CaP nanoparticles for siRNA delivery. These nanoparticles are mainly classified into lipid coated, polymer coated and various other types for discussion.
authors.affiliation: Institute of Biomaterials and Marine Biological Resources, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China;
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Xu
authors.affiliation: Institute of Biomaterials and Marine Biological Resources, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China;
authors.forename: Zehao
authors.initials: Z
authors.lastname: Li
authors.affiliation: Institute of Biomaterials and Marine Biological Resources, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China;
authors.forename: Xueqin
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Institute of Biomaterials and Marine Biological Resources, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China;
authors.forename: Lawrence
authors.initials: L
authors.lastname: Keen
authors.affiliation: Institute of Biomaterials and Marine Biological Resources, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China;; College of Materials and Textiles, Zhejiang Sci-Tech University, Hangzhou 310018, China.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Kong
keywords: calcium phosphate; delivery; nanoparticles; siRNA