title: NCW2, a Gene Involved in the Tolerance to Polyhexamethylene Biguanide (PHMB), May Help in the Organisation of β-1,3-Glucan Structure of Saccharomyces cerevisiae Cell Wall.
abstract: In the present work, we provide biological evidences supporting the participation of NCW2 gene in the mechanism responsible for cell tolerance to polyhexamethylene biguanide (PHMB), an antifungal agent. The growth rate of yeast cells exposed to this agent was significantly reduced in ∆ncw2 strain and the mRNA levels of NCW2 gene in the presence of PHMB showed a 7-fold up-regulation. Moreover, lack of NCW2 gene turns yeast cell more resistant to zymolyase treatment, indicating that alterations in the β-glucan network do occur when Ncw2p is absent. Computational analysis of the translated protein indicated neither catalytic nor transmembrane sites and reinforced the hypothesis of secretion and anchoring to cell surface. Altogether, these results indicated that NCW2 gene codes for a protein which participates in the cell wall biogenesis in yeasts and that Ncw2p might play a role in the organisation of the β-glucan assembly.
authors.affiliation: Interdepartmental Research Group in Metabolic Engineering, Federal University of Pernambuco, Recife, 50670-901, Brazil.|Department of Biochemistry, Federal University of Pernambuco, Recife, 50670-901, Brazil.
authors.forename: Carolina
authors.initials: C
authors.lastname: Elsztein
authors.affiliation: Interdepartmental Research Group in Metabolic Engineering, Federal University of Pernambuco, Recife, 50670-901, Brazil.
authors.forename: Rita de Cássia Pereira
authors.initials: RCP
authors.lastname: de Lima
authors.affiliation: Interdepartmental Research Group in Metabolic Engineering, Federal University of Pernambuco, Recife, 50670-901, Brazil.|Department of Antibiotics, Federal University of Pernambuco, Recife, 50670-901, Brazil.
authors.forename: Will
authors.initials: W
authors.lastname: de Barros Pita
authors.affiliation: Interdepartmental Research Group in Metabolic Engineering, Federal University of Pernambuco, Recife, 50670-901, Brazil. marcos.moraisjr@ufpe.br.|Department of Genetics, Universidade Federal de Pernambuco, Av. Moraes Rego, 1235 Cidade Universitária, Recife, PE, 50670-901, Brazil. marcos.moraisjr@ufpe.br.
authors.forename: Marcos Antonio
authors.initials: MA
authors.lastname: de Morais
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D001645:Biguanides / Q000494:pharmacology*; D002473:Cell Wall / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D025141:Drug Resistance, Fungal; D015966:Gene Expression Regulation, Fungal; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D047071:beta-Glucans / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000935:Antifungal Agents; D001645:Biguanides; D008565:Membrane Proteins; C000613676:NCW2 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D047071:beta-Glucans; C031233:polihexanide