title: Development, validation and application of a sensitive analytical method for residue determination and dissipation of imidacloprid in sugarcane under tropical field condition.
abstract: A simple and sensitive analytical method has been developed and validated for the determination of trace amounts of imidacloprid in/on sugarcane sett, stalk and leaf. The method optimized in the present study requires less volume of organic solvent and time. Hence, this method is suitable for high-throughput analyses involving large number of samples. The limit of detection (LOD) and limit of quantification (LOQ) of the method were 0.003 and 0.01 mg/kg, respectively. The recovery and relative standard deviation were more than 93 % and less than 4 %, respectively. Thus, it is obvious that the analytical method standardized in this study is more precise and accurate enough to determine the residues of imidacloprid in sugarcane sett, stalk and leaf. The dissipation and translocation of imidacloprid residues from treated cane setts to leaf and stalk were studied by adopting this method. In sugarcane setts, the residues of imidacloprid persisted up to 120 days with half-life of 15.4 days at its recommended dose (70 g a.i./ha). The residues of imidacloprid were found to be translocated from setts to stalk and leaf. The imidacloprid residues were detected up to 105 days in both leaf and stalk. Dipping of sugarcane setts in imidacloprid at its recommended dose may result in better protection of cane setts and established crop because of higher initial deposit (>100 mg/kg) and longer persistence (>120 days).
authors.affiliation: Division of Crop Protection, ICAR-Sugarcane Breeding Institute, Indian Council of Agricultural Research, Coimbatore, Tamil Nadu State, 641007, India. tramasubbu@gmail.com.
authors.forename: T
authors.initials: T
authors.lastname: Ramasubramanian
authors.affiliation: Pesticide Toxicology Laboratory, Department of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore, 641003, India.
authors.forename: M
authors.initials: M
authors.lastname: Paramasivam
authors.affiliation: Division of Crop Protection, ICAR-Sugarcane Breeding Institute, Indian Council of Agricultural Research, Coimbatore, Tamil Nadu State, 641007, India.
authors.forename: R
authors.initials: R
authors.lastname: Nirmala
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004784:Environmental Monitoring / Q000379:methods*; D006207:Half-Life; D007093:Imidazoles / Q000032:analysis*; D007194:India; D007306:Insecticides / Q000032:analysis*; D057230:Limit of Detection; D000073943:Neonicotinoids; D009574:Nitro Compounds / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry; D031786:Saccharum / Q000737:chemistry*; D014329:Tropical Climate
chemical_list: D007093:Imidazoles; D007306:Insecticides; D000073943:Neonicotinoids; D009574:Nitro Compounds; D010573:Pesticide Residues; C082359:imidacloprid
keywords: Dissipation; Half-life; Imidacloprid; Residue; Sugarcane