title: In Situ Detection of Protein Complexes and Modifications by Chemical Ligation Proximity Assay.
abstract: Protein function is often regulated by protein-protein interactions and post-translational modifications. Detection of these important biological phenomena in fixed biological samples could serve as an invaluable tool in biomedical research, drug development, as well as clinical cancer diagnostics and prognostics. We report here a novel methodology which utilizes unique antibody bioconjugates capable of forming proximity induced chemical ligation to enable in situ detection of proximal targets in fixed biological samples. Using this new methodology, we demonstrate in situ visualization of various protein heterodimers/complexes and post-translational modifications such as phosphorylation and ubiquitination. This new method offers high specificity, sensitivity, flexibility, and ease of use. In addition, the assay preserves critical contextual and heterogeneity information on biomarkers in clinically relevant samples.
authors.affiliation: Technology and Applied Research, Ventana Medical Systems, Inc. , Tucson, Arizona 85755, United States.
authors.forename: Rui
authors.initials: R
authors.lastname: Hong
authors.affiliation: Technology and Applied Research, Ventana Medical Systems, Inc. , Tucson, Arizona 85755, United States.
authors.forename: Esteban
authors.initials: E
authors.lastname: Roberts
authors.affiliation: Technology and Applied Research, Ventana Medical Systems, Inc. , Tucson, Arizona 85755, United States.
authors.forename: Christopher
authors.initials: C
authors.lastname: Bieniarz
mesh_terms: D006367:HeLa Cells; D006801:Humans; D010766:Phosphorylation; D055503:Protein Multimerization; D011499:Protein Processing, Post-Translational; D020836:Protein Structure, Quaternary; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D054875:Ubiquitination
chemical_list: D011506:Proteins