title: Defective Pollen Wall 2 (DPW2) Encodes an Acyl Transferase Required for Rice Pollen Development.
abstract: Aliphatic and aromatic lipids are both essential structural components of the plant cuticle, an important interface between the plant and environment. Although cross links between aromatic and aliphatic or other moieties are known to be associated with the formation of leaf cutin and root and seed suberin, the contribution of aromatic lipids to the biosynthesis of anther cuticles and pollen walls remains elusive. In this study, we characterized the rice (Oryza sativa) male sterile mutant, defective pollen wall 2 (dpw2), which showed an abnormal anther cuticle, a defective pollen wall, and complete male sterility. Compared with the wild type, dpw2 anthers have increased amounts of cutin and waxes and decreased levels of lipidic and phenolic compounds. DPW2 encodes a cytoplasmically localized BAHD acyltransferase. In vitro assays demonstrated that recombinant DPW2 specifically transfers hydroxycinnamic acid moieties, using ω-hydroxy fatty acids as acyl acceptors and hydroxycinnamoyl-CoAs as acyl donors. Thus, The cytoplasmic hydroxycinnamoyl-CoA:ω-hydroxy fatty acid transferase DPW2 plays a fundamental role in male reproduction via the biosynthesis of key components of the anther cuticle and pollen wall.
authors.affiliation: Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China (D.X., J.S., L.Y., X.Q., M.U., L.Z., Q.L., D.Z., W.L.).|ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Victoria 3010, Australia (C.R., J.L.H.).|Joint BioEnergy Institute and Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720 (C.R., J.L.H., H.V.S.).|Crop Biotech Institute and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Korea (G.A.).|Institute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (F.W., L.S.).|Department of Plant and Microbial Biology, University of California, Berkeley, California 94720 (H.V.S.).|Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824 (J.H.); and.|School of Agriculture, Food and Wine, University of Adelaide, Urrbrae, South Australia 5064, Australia (D.Z.).
authors.forename: Dawei
authors.identifier: 0000-0002-8906-7070
authors.initials: D
authors.lastname: Xu
authors.affiliation: Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China (D.X., J.S., L.Y., X.Q., M.U., L.Z., Q.L., D.Z., W.L.).|ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Victoria 3010, Australia (C.R., J.L.H.).|Joint BioEnergy Institute and Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720 (C.R., J.L.H., H.V.S.).|Crop Biotech Institute and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Korea (G.A.).|Institute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (F.W., L.S.).|Department of Plant and Microbial Biology, University of California, Berkeley, California 94720 (H.V.S.).|Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824 (J.H.); and.|School of Agriculture, Food and Wine, University of Adelaide, Urrbrae, South Australia 5064, Australia (D.Z.).
authors.forename: Jianxin
authors.initials: J
authors.lastname: Shi
authors.affiliation: Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China (D.X., J.S., L.Y., X.Q., M.U., L.Z., Q.L., D.Z., W.L.).|ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Victoria 3010, Australia (C.R., J.L.H.).|Joint BioEnergy Institute and Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720 (C.R., J.L.H., H.V.S.).|Crop Biotech Institute and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Korea (G.A.).|Institute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (F.W., L.S.).|Department of Plant and Microbial Biology, University of California, Berkeley, California 94720 (H.V.S.).|Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824 (J.H.); and.|School of Agriculture, Food and Wine, University of Adelaide, Urrbrae, South Australia 5064, Australia (D.Z.).
authors.forename: Carsten
authors.initials: C
authors.lastname: Rautengarten
authors.affiliation: Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China (D.X., J.S., L.Y., X.Q., M.U., L.Z., Q.L., D.Z., W.L.).|ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Victoria 3010, Australia (C.R., J.L.H.).|Joint BioEnergy Institute and Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720 (C.R., J.L.H., H.V.S.).|Crop Biotech Institute and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Korea (G.A.).|Institute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (F.W., L.S.).|Department of Plant and Microbial Biology, University of California, Berkeley, California 94720 (H.V.S.).|Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824 (J.H.); and.|School of Agriculture, Food and Wine, University of Adelaide, Urrbrae, South Australia 5064, Australia (D.Z.).
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.affiliation: Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China (D.X., J.S., L.Y., X.Q., M.U., L.Z., Q.L., D.Z., W.L.).|ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Victoria 3010, Australia (C.R., J.L.H.).|Joint BioEnergy Institute and Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720 (C.R., J.L.H., H.V.S.).|Crop Biotech Institute and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Korea (G.A.).|Institute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (F.W., L.S.).|Department of Plant and Microbial Biology, University of California, Berkeley, California 94720 (H.V.S.).|Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824 (J.H.); and.|School of Agriculture, Food and Wine, University of Adelaide, Urrbrae, South Australia 5064, Australia (D.Z.).
authors.forename: Xiaoling
authors.identifier: 0000-0002-4173-5762
authors.initials: X
authors.lastname: Qian
authors.affiliation: Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China (D.X., J.S., L.Y., X.Q., M.U., L.Z., Q.L., D.Z., W.L.).|ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Victoria 3010, Australia (C.R., J.L.H.).|Joint BioEnergy Institute and Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720 (C.R., J.L.H., H.V.S.).|Crop Biotech Institute and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Korea (G.A.).|Institute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (F.W., L.S.).|Department of Plant and Microbial Biology, University of California, Berkeley, California 94720 (H.V.S.).|Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824 (J.H.); and.|School of Agriculture, Food and Wine, University of Adelaide, Urrbrae, South Australia 5064, Australia (D.Z.).
authors.forename: Muhammad
authors.initials: M
authors.lastname: Uzair
authors.affiliation: Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China (D.X., J.S., L.Y., X.Q., M.U., L.Z., Q.L., D.Z., W.L.).|ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Victoria 3010, Australia (C.R., J.L.H.).|Joint BioEnergy Institute and Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720 (C.R., J.L.H., H.V.S.).|Crop Biotech Institute and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Korea (G.A.).|Institute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (F.W., L.S.).|Department of Plant and Microbial Biology, University of California, Berkeley, California 94720 (H.V.S.).|Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824 (J.H.); and.|School of Agriculture, Food and Wine, University of Adelaide, Urrbrae, South Australia 5064, Australia (D.Z.).
authors.forename: Lu
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China (D.X., J.S., L.Y., X.Q., M.U., L.Z., Q.L., D.Z., W.L.).|ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Victoria 3010, Australia (C.R., J.L.H.).|Joint BioEnergy Institute and Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720 (C.R., J.L.H., H.V.S.).|Crop Biotech Institute and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Korea (G.A.).|Institute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (F.W., L.S.).|Department of Plant and Microbial Biology, University of California, Berkeley, California 94720 (H.V.S.).|Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824 (J.H.); and.|School of Agriculture, Food and Wine, University of Adelaide, Urrbrae, South Australia 5064, Australia (D.Z.).
authors.forename: Qian
authors.initials: Q
authors.lastname: Luo
authors.affiliation: Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China (D.X., J.S., L.Y., X.Q., M.U., L.Z., Q.L., D.Z., W.L.).|ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Victoria 3010, Australia (C.R., J.L.H.).|Joint BioEnergy Institute and Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720 (C.R., J.L.H., H.V.S.).|Crop Biotech Institute and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Korea (G.A.).|Institute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (F.W., L.S.).|Department of Plant and Microbial Biology, University of California, Berkeley, California 94720 (H.V.S.).|Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824 (J.H.); and.|School of Agriculture, Food and Wine, University of Adelaide, Urrbrae, South Australia 5064, Australia (D.Z.).
authors.forename: Gynheung
authors.initials: G
authors.lastname: An
authors.affiliation: Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China (D.X., J.S., L.Y., X.Q., M.U., L.Z., Q.L., D.Z., W.L.).|ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Victoria 3010, Australia (C.R., J.L.H.).|Joint BioEnergy Institute and Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720 (C.R., J.L.H., H.V.S.).|Crop Biotech Institute and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Korea (G.A.).|Institute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (F.W., L.S.).|Department of Plant and Microbial Biology, University of California, Berkeley, California 94720 (H.V.S.).|Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824 (J.H.); and.|School of Agriculture, Food and Wine, University of Adelaide, Urrbrae, South Australia 5064, Australia (D.Z.).
authors.forename: Fritz
authors.initials: F
authors.lastname: Waßmann
authors.affiliation: Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China (D.X., J.S., L.Y., X.Q., M.U., L.Z., Q.L., D.Z., W.L.).|ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Victoria 3010, Australia (C.R., J.L.H.).|Joint BioEnergy Institute and Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720 (C.R., J.L.H., H.V.S.).|Crop Biotech Institute and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Korea (G.A.).|Institute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (F.W., L.S.).|Department of Plant and Microbial Biology, University of California, Berkeley, California 94720 (H.V.S.).|Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824 (J.H.); and.|School of Agriculture, Food and Wine, University of Adelaide, Urrbrae, South Australia 5064, Australia (D.Z.).
authors.forename: Lukas
authors.identifier: 0000-0001-7003-9929
authors.initials: L
authors.lastname: Schreiber
authors.affiliation: Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China (D.X., J.S., L.Y., X.Q., M.U., L.Z., Q.L., D.Z., W.L.).|ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Victoria 3010, Australia (C.R., J.L.H.).|Joint BioEnergy Institute and Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720 (C.R., J.L.H., H.V.S.).|Crop Biotech Institute and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Korea (G.A.).|Institute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (F.W., L.S.).|Department of Plant and Microbial Biology, University of California, Berkeley, California 94720 (H.V.S.).|Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824 (J.H.); and.|School of Agriculture, Food and Wine, University of Adelaide, Urrbrae, South Australia 5064, Australia (D.Z.).
authors.forename: Joshua L
authors.identifier: 0000-0002-2080-3826
authors.initials: JL
authors.lastname: Heazlewood
authors.affiliation: Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China (D.X., J.S., L.Y., X.Q., M.U., L.Z., Q.L., D.Z., W.L.).|ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Victoria 3010, Australia (C.R., J.L.H.).|Joint BioEnergy Institute and Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720 (C.R., J.L.H., H.V.S.).|Crop Biotech Institute and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Korea (G.A.).|Institute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (F.W., L.S.).|Department of Plant and Microbial Biology, University of California, Berkeley, California 94720 (H.V.S.).|Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824 (J.H.); and.|School of Agriculture, Food and Wine, University of Adelaide, Urrbrae, South Australia 5064, Australia (D.Z.).
authors.forename: Henrik Vibe
authors.identifier: 0000-0002-6702-3560
authors.initials: HV
authors.lastname: Scheller
authors.affiliation: Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China (D.X., J.S., L.Y., X.Q., M.U., L.Z., Q.L., D.Z., W.L.).|ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Victoria 3010, Australia (C.R., J.L.H.).|Joint BioEnergy Institute and Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720 (C.R., J.L.H., H.V.S.).|Crop Biotech Institute and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Korea (G.A.).|Institute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (F.W., L.S.).|Department of Plant and Microbial Biology, University of California, Berkeley, California 94720 (H.V.S.).|Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824 (J.H.); and.|School of Agriculture, Food and Wine, University of Adelaide, Urrbrae, South Australia 5064, Australia (D.Z.).
authors.forename: Jianping
authors.identifier: 0000-0002-4635-4299
authors.initials: J
authors.lastname: Hu
authors.affiliation: Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China (D.X., J.S., L.Y., X.Q., M.U., L.Z., Q.L., D.Z., W.L.).|ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Victoria 3010, Australia (C.R., J.L.H.).|Joint BioEnergy Institute and Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720 (C.R., J.L.H., H.V.S.).|Crop Biotech Institute and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Korea (G.A.).|Institute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (F.W., L.S.).|Department of Plant and Microbial Biology, University of California, Berkeley, California 94720 (H.V.S.).|Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824 (J.H.); and.|School of Agriculture, Food and Wine, University of Adelaide, Urrbrae, South Australia 5064, Australia (D.Z.).
authors.forename: Dabing
authors.identifier: 0000-0002-1764-2929
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China (D.X., J.S., L.Y., X.Q., M.U., L.Z., Q.L., D.Z., W.L.); wqliang@sjtu.edu.cn.|ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Victoria 3010, Australia (C.R., J.L.H.); wqliang@sjtu.edu.cn.|Joint BioEnergy Institute and Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720 (C.R., J.L.H., H.V.S.); wqliang@sjtu.edu.cn.|Crop Biotech Institute and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Korea (G.A.); wqliang@sjtu.edu.cn.|Institute of Cellular and Molecular Botany, University of Bonn, D-53115 Bonn, Germany (F.W., L.S.); wqliang@sjtu.edu.cn.|Department of Plant and Microbial Biology, University of California, Berkeley, California 94720 (H.V.S.); wqliang@sjtu.edu.cn.|Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824 (J.H.); and wqliang@sjtu.edu.cn.|School of Agriculture, Food and Wine, University of Adelaide, Urrbrae, South Australia 5064, Australia (D.Z.) wqliang@sjtu.edu.cn.
authors.forename: Wanqi
authors.identifier: 0000-0002-9938-5793
authors.initials: W
authors.lastname: Liang
mesh_terms: D000217:Acyltransferases / Q000378:metabolism*; D000595:Amino Acid Sequence; D002473:Cell Wall / Q000378:metabolism; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D050356:Lipid Metabolism; D008563:Membrane Lipids / Q000378:metabolism; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D012275:Oryza / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development* / Q000648:ultrastructure; D010636:Phenols / Q000378:metabolism; D010641:Phenotype; D010940:Plant Proteins / Q000378:metabolism*; D011058:Pollen / Q000201:enzymology* / Q000254:growth & development* / Q000648:ultrastructure; D021381:Protein Transport; D011994:Recombinant Proteins / Q000378:metabolism; D020539:Sequence Analysis, Protein; D014885:Waxes / Q000378:metabolism
chemical_list: D008563:Membrane Lipids; D010636:Phenols; D010940:Plant Proteins; D011994:Recombinant Proteins; D014885:Waxes; C000521:cutin; D000217:Acyltransferases