title: Design of an enzyme cocktail consisting of different fungal platforms for efficient hydrolysis of sugarcane bagasse: Optimization and synergism studies.
abstract: Lignocellulosic materials represent a very important and promising source of renewable biomass. In order to turn them into fermentable sugars, synergism among the different enzymes that carry out bioconversion of these materials is one of the main factors that should be considered. Experimental mixture design was performed to optimize the proportion of enzymes produced by native strains of Trichoderma harzianum IOC 3844, Penicillium funiculosum ATCC 11797, and Aspergillus niger ATCC 1004, resulting in a proportion of 15, 50, and 35%, respectively. This mixture was able to hydrolyze 25 g/L of pretreated sugarcane bagasse with 91% of yield after 48 h of enzymatic reaction. Synergism along the hydrolysis process, besides the influence of lignin, hemicellulose, and solids loading, were also studied. Response surface methodology (RSM) based on Central Composite Rotatable Design was used to optimize solids and protein loadings to increase glucose release and enzymatic hydrolysis yield. The optimum solid and protein loadings established with RSM were 196 g/L and 24 mg/g cellulose, respectively, and under these conditions (94.1 ± 8) g/L of glucose were obtained, corresponding to a hydrolysis yield of 64%. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1222-1229, 2016.
authors.affiliation: Laboratórios de Desenvolvimento de Bioprocessos, Departamento de Engenharia Bioquímica, Escola de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21949-900, Brazil.
authors.forename: Johanna
authors.initials: J
authors.lastname: Méndez Arias
authors.affiliation: Laboratórios de Desenvolvimento de Bioprocessos, Departamento de Engenharia Bioquímica, Escola de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21949-900, Brazil.
authors.forename: Luiz Felipe Amarante
authors.initials: LF
authors.lastname: Modesto
authors.affiliation: Instituto de Física de São Carlos, Departamento de Física e Ciência Interdisciplinar, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Igor
authors.initials: I
authors.lastname: Polikarpov
authors.affiliation: Laboratórios de Desenvolvimento de Bioprocessos, Departamento de Engenharia Bioquímica, Escola de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21949-900, Brazil. nei@eq.ufrj.br.
authors.forename: Nei
authors.initials: N
authors.lastname: Pereira
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology*; D044602:Cellulases / Q000096:biosynthesis / Q000378:metabolism*; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism*; D006868:Hydrolysis; D010407:Penicillium / Q000201:enzymology*; D031786:Saccharum / Q000737:chemistry* / Q000378:metabolism; D014242:Trichoderma / Q000201:enzymology*
chemical_list: D002482:Cellulose; C027433:bagasse; D044602:Cellulases
keywords: cellulase; enzymatic cocktails; enzymatic hydrolysis; experimental mixture design; synergism