title: Sex-determining region Y-box3 (SOX3) functions as an oncogene in promoting epithelial ovarian cancer by targeting Src kinase.
abstract: Ovarian cancer is one of the most common cancers which cause female mortality. The knowledge of ovarian cancer initiation and progression is critical to develop new therapeutic strategies to treat and prevent it. Recently, SOX3 has been reported to play a pivotal role in tumor progression. However, the clinical significance of SOX3 in human ovarian cancer remains elusive, and the identity of SOX3 in ovarian cancer initiation, progression, and the related underlying mechanism is unknown. In this study, we showed that SOX3 expression increased from benign and borderline to malignant ovarian tumors. Subsequently, we found that overexpression of SOX3 in EOC cells promoted proliferation, migration, and invasion, while restrained apoptosis and adhesion of ovarian cancer cells. In contrast, silencing of SOX3 gained the opposite results. Finally, we discovered SOX3 targeted Src kinase in EOC cells. These data imply that SOX3, acting as an oncogene in EOC, is not only a crucial factor in the carcinogenesis but also a promising therapeutic target for EOC.
authors.affiliation: Department of Gynecology and Obstetrics, Shanghai General Hospital Affiliated to Shanghai JiaoTong University School of Medicine, No. 650, Xin Songjiang Road, Shanghai, 201260, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Yan
authors.affiliation: Department of Gynecology and Obstetrics, Shanghai General Hospital Affiliated to Shanghai JiaoTong University School of Medicine, No. 650, Xin Songjiang Road, Shanghai, 201260, China.
authors.forename: Fangyuan
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Gynecology and Obstetrics, Shanghai General Hospital Affiliated to Shanghai JiaoTong University School of Medicine, No. 650, Xin Songjiang Road, Shanghai, 201260, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Miao
authors.affiliation: Department of Gynecology and Obstetrics, Shanghai General Hospital Affiliated to Shanghai JiaoTong University School of Medicine, No. 650, Xin Songjiang Road, Shanghai, 201260, China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Gynecology and Obstetrics, Shanghai General Hospital Affiliated to Shanghai JiaoTong University School of Medicine, No. 650, Xin Songjiang Road, Shanghai, 201260, China.
authors.forename: Mingzhu
authors.initials: M
authors.lastname: Bai
authors.affiliation: Department of Gynecology and Obstetrics, Shanghai General Hospital Affiliated to Shanghai JiaoTong University School of Medicine, No. 650, Xin Songjiang Road, Shanghai, 201260, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Xi
authors.affiliation: Department of Gynecology and Obstetrics, Shanghai General Hospital Affiliated to Shanghai JiaoTong University School of Medicine, No. 650, Xin Songjiang Road, Shanghai, 201260, China. fengyj4806@126.com.
authors.forename: Youji
authors.initials: Y
authors.lastname: Feng
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis / Q000235:genetics; D015153:Blotting, Western; D000077216:Carcinoma, Ovarian Epithelial; D002448:Cell Adhesion / Q000235:genetics; D002460:Cell Line; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D008875:Middle Aged; D009375:Neoplasms, Glandular and Epithelial / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D009857:Oncogenes / Q000235:genetics; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055748:SOXB1 Transcription Factors / Q000235:genetics* / Q000378:metabolism; D055815:Young Adult; D019061:src-Family Kinases / Q000235:genetics* / Q000378:metabolism
chemical_list: C527785:SOX3 protein, human; D055748:SOXB1 Transcription Factors; D019061:src-Family Kinases
keywords: Epithelial ovarian cancer; Invasion; Migration; Proliferation; SOX3; Src