title: A method to rapidly create protein aggregates in living cells.
abstract: The accumulation of protein aggregates is a common pathological hallmark of many neurodegenerative diseases. However, we do not fully understand how aggregates are formed or the complex network of chaperones, proteasomes and other regulatory factors involved in their clearance. Here, we report a chemically controllable fluorescent protein that enables us to rapidly produce small aggregates inside living cells on the order of seconds, as well as monitor the movement and coalescence of individual aggregates into larger structures. This method can be applied to diverse experimental systems, including live animals, and may prove valuable for understanding cellular responses and diseases associated with protein aggregates.
authors.affiliation: Department of Chemical &Systems Biology Stanford University, Stanford, California 94305, USA.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Miyazaki
authors.affiliation: Department of Biology Stanford University, Stanford, California 94305, USA.
authors.forename: Kota
authors.initials: K
authors.lastname: Mizumoto
authors.affiliation: Department of Chemical &Systems Biology Stanford University, Stanford, California 94305, USA.
authors.forename: Gautam
authors.identifier: 0000-0003-1416-6223
authors.initials: G
authors.lastname: Dey
authors.affiliation: Department of Chemical &Systems Biology Stanford University, Stanford, California 94305, USA.
authors.forename: Takamasa
authors.initials: T
authors.lastname: Kudo
authors.affiliation: Cell Sciences Imaging Facility Stanford University, Stanford, California 94305, USA.
authors.forename: John
authors.initials: J
authors.lastname: Perrino
authors.affiliation: Department of Chemical &Systems Biology Stanford University, Stanford, California 94305, USA.
authors.forename: Ling-Chun
authors.initials: LC
authors.lastname: Chen
authors.affiliation: Department of Chemical &Systems Biology Stanford University, Stanford, California 94305, USA.
authors.forename: Tobias
authors.initials: T
authors.lastname: Meyer
authors.affiliation: Department of Chemical &Systems Biology Stanford University, Stanford, California 94305, USA.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Wandless
mesh_terms: D000818:Animals; D001665:Binding Sites / Q000235:genetics; D017173:Caenorhabditis elegans / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D041681:NIH 3T3 Cells; D066329:Protein Aggregates; D066263:Protein Aggregation, Pathological; D022061:Tacrolimus Binding Protein 1A / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D059008:Time-Lapse Imaging / Q000379:methods
chemical_list: D066329:Protein Aggregates; D049452:Green Fluorescent Proteins; D022061:Tacrolimus Binding Protein 1A