title: Molecular determinant modulates thermal recovery kinetics and structural integrity of the bacterial BLUF photoreceptor.
abstract: BLUF domains are flavin-based photoreceptors which receive the blue light signal and are involved in the sensory transduction. We report a short BLUF photoreceptor (SnfB) in Stenotrophomonas sp. We have investigated photodynamic properties of C terminus truncated and several mutated SnfB proteins. Deletion of the extended C-terminal residues alters the thermal recovery kinetics and also affects the integrity of the SnfB protein. Mutagenesis studies demonstrated that the conserved residues within and outside the flavin-binding pocket also regulates the photocycle properties of the protein. These studies suggest that the C-terminal residues outside the BLUF domain can tune the photodynamic properties of the BLUF protein.
authors.affiliation: Department of Biochemistry, University of Delhi, New Delhi, India.
authors.forename: Meenakshi
authors.initials: M
authors.lastname: Tanwar
authors.affiliation: Department of Biochemistry, University of Delhi, New Delhi, India.
authors.forename: Smita
authors.initials: S
authors.lastname: Nahar
authors.affiliation: Department of Biochemistry, University of Delhi, New Delhi, India.
authors.forename: Sahil
authors.initials: S
authors.lastname: Gulati
authors.affiliation: School of Biotechnology, Jawaharlal Nehru University, New Delhi, India.
authors.forename: Sindhu Kandoth
authors.initials: SK
authors.lastname: Veetil
authors.affiliation: Department of Biochemistry, University of Delhi, New Delhi, India.|School of Biotechnology, Jawaharlal Nehru University, New Delhi, India.
authors.forename: Suneel
authors.initials: S
authors.lastname: Kateriya
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D016296:Mutagenesis; D000072417:Protein Domains; D020588:Stenotrophomonas / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins
keywords: BLUF; C terminus; Stenotrophomonas sp. SKA14; dark recovery kinetics; photoreceptors