title: Plasma metabolomic profile and potential biomarkers for missed abortion.
abstract: A missed abortion (MA) is an in utero death of the embryo or fetus before the 20th week of gestation with retained products of conception, and this condition is currently common in China. In order to discover novel biomarkers for MA, ultrahigh performance liquid chromatography was applied to study plasma metabolite profiles for 33 patients with MA and 29 control subjects. Thirty-seven differential plasma metabolites were found to discriminate between the two groups in the initial cohort (15 subjects with MA and 15 healthy controls). The feasibility of using these potential biomarkers to predict MA was further evaluated in the validation cohort (18 subjects with MA and 14 healthy controls) and 15 had an area under the receiver operating characteristic curve of >0.80, making them satisfactory. Tryptophan metabolism and sphingolipid metabolism were identified as important potential target pathways for MA using metabolic pathway impact analysis. Furthermore, three of the 15 satisfactory metabolites (glyceric acid, indole and sphingosine) were combined to establish a predictive model with 100% sensitivity and 100% specificity in the validation cohort. Taken together, these results suggest that MA results in significant disturbance of metabolism and those various novel biomarkers have satisfactory diagnostic and predictive power for MA.
authors.affiliation: Department of Obstetrics and Gynaecology, The Fifth People's Hospital of Shanghai, School of Medicine, Fudan University, Shanghai, People's Republic of China.
authors.forename: He
authors.initials: H
authors.lastname: Fei
authors.affiliation: Second Military Medical University, Shanghai, People's Republic of China.
authors.forename: Jiebin
authors.initials: J
authors.lastname: Hou
authors.affiliation: Department of Obstetrics and Gynaecology, The Fifth People's Hospital of Shanghai, School of Medicine, Fudan University, Shanghai, People's Republic of China.
authors.forename: Zhenghong
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Department of Obstetrics and Gynaecology, The Fifth People's Hospital of Shanghai, School of Medicine, Fudan University, Shanghai, People's Republic of China.
authors.forename: Liwen
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Second Military Medical University, Shanghai, People's Republic of China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Second Military Medical University, Shanghai, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Dong
authors.affiliation: Department of Obstetrics and Gynaecology, The Fifth People's Hospital of Shanghai, School of Medicine, Fudan University, Shanghai, People's Republic of China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000022:Abortion, Spontaneous; D015415:Biomarkers; D016022:Case-Control Studies; D002853:Chromatography, Liquid; D005260:Female; D006801:Humans; D055432:Metabolomics; D011247:Pregnancy; D012015:Reference Standards
chemical_list: D015415:Biomarkers
keywords: biomarker; liquid chromatography-mass spectrometry; metabolomics; missed abortion