title: Adaption of microbial community during the start-up stage of a thermophilic anaerobic digester treating food waste.
abstract: A successful start-up enables acceleration of anaerobic digestion (AD) into steady state. The microbial community influences the AD performance during the start-up. To investigate how microbial communities changed during the start-up, microbial dynamics was analyzed via high-throughput sequencing in this study. The results confirmed that the AD was started up within 25 d. Thermophilic methanogens and bacterial members functioning in hydrolysis, acidogenesis, and syntrophic oxidation became predominant during the start-up stage, reflecting a quick adaption of microorganisms to operating conditions. Such predominance also indicated the great contribution of these members to the fast start-up of AD. Redundancy analysis confirmed that the bacterial abundance significantly correlated with AD conditions. The stable ratio of hydrogenotrophic methanogens to aceticlastic methanogens is also important to maintain the stability of the AD process. This work will be helpful to understand the contribution of microbial community to the start-up of AD.
authors.affiliation: a Biogas Institute of Ministry of Agriculture , Chengdu , China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wu
authors.affiliation: a Biogas Institute of Ministry of Agriculture , Chengdu , China.|b Graduate School of Chinese Academy of Agricultural Sciences , Beijing , China.
authors.forename: Xing
authors.initials: X
authors.lastname: Wang
authors.affiliation: a Biogas Institute of Ministry of Agriculture , Chengdu , China.
authors.forename: Ya-Yue
authors.initials: YY
authors.lastname: Deng
authors.affiliation: c Institute of Soil and Fertilizer , Sichuan Academy of Agricultural Sciences , Chengdu , China.
authors.forename: Xiao-Lan
authors.initials: XL
authors.lastname: He
authors.affiliation: a Biogas Institute of Ministry of Agriculture , Chengdu , China.|b Graduate School of Chinese Academy of Agricultural Sciences , Beijing , China.
authors.forename: Zheng-Wei
authors.initials: ZW
authors.lastname: Li
authors.affiliation: a Biogas Institute of Ministry of Agriculture , Chengdu , China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: a Biogas Institute of Ministry of Agriculture , Chengdu , China.
authors.forename: Han
authors.initials: H
authors.lastname: Qin
authors.affiliation: a Biogas Institute of Ministry of Agriculture , Chengdu , China.|b Graduate School of Chinese Academy of Agricultural Sciences , Beijing , China.
authors.forename: Jing-Tao
authors.initials: JT
authors.lastname: Chen
authors.affiliation: a Biogas Institute of Ministry of Agriculture , Chengdu , China.
authors.forename: Ming-Xiong
authors.initials: MX
authors.lastname: He
authors.affiliation: a Biogas Institute of Ministry of Agriculture , Chengdu , China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: a Biogas Institute of Ministry of Agriculture , Chengdu , China.
authors.forename: Guo-Quan
authors.initials: GQ
authors.lastname: Hu
authors.affiliation: a Biogas Institute of Ministry of Agriculture , Chengdu , China.
authors.forename: Xiao-Bo
authors.initials: XB
authors.lastname: Yin
mesh_terms: D000222:Adaptation, Physiological; D000693:Anaerobiosis; D001105:Archaea / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism* / Q000502:physiology; D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D018407:Bacterial Physiological Phenomena; D005502:Food; D059014:High-Throughput Nucleotide Sequencing; D007700:Kinetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014866:Waste Products
chemical_list: D012336:RNA, Ribosomal, 16S; D014866:Waste Products
keywords: 16S rRNA high-throughput sequencing; food waste; microbial community dynamics; start-up; thermophilic anaerobic digestion