title: Curcumin Promotes Osteosarcoma Cell Death by Activating miR-125a/ERRα Signal Pathway.
abstract: Curcumin has demonstrated valuable therapeutic potential against a variety of human cancers including osteosarcoma. However, the molecular mechanisms underlying its anti-tumor effect remain to be poorly understood. By RNA sequence profiling, we found that curcumin significantly down-regulates the expression of estrogen-related receptor alpha (ERRα) in osteosarcoma cells. Overexpression of ERRα diminished curcumin-activated apoptotic cell death and scavenged curcumin-induced reactive oxygen species (ROS), while ERRα silencing sensitized osteosarcoma cells to curcumin, resulting in increased inhibition of cell proliferation. In addition, we found that curcumin suppressed the ERRα gene expression through upregulation of miR-125a. Data from this study revealed a novel mechanism for curcumin-mediated apoptotic cell death, which involves tumor cell killing via activating miR-125a/ERRα pathway. Our studies also provide further support for osteosarcoma therapy by targeting ERRα alone or in combination with curcumin. J. Cell. Biochem. 118: 74-81, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: First Affiliated Hospital, Guangzhou University of Chinese Medicine, 16 Jichang Road, Guangzhou 510405, People's Republic of China.|Department of Biochemistry and Molecular Medicine, Comprehensive Cancer Center, University of California at Davis, 4645 2nd Avenue, Sacramento 95817, California.|Guangzhou University of Chinese Medicine, 12 Jichang Road, Guangzhou 510405, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Chen
authors.affiliation: First Affiliated Hospital, Guangzhou University of Chinese Medicine, 16 Jichang Road, Guangzhou 510405, People's Republic of China.|Guangzhou University of Chinese Medicine, 12 Jichang Road, Guangzhou 510405, People's Republic of China.
authors.forename: Haibin
authors.initials: H
authors.lastname: Wang
authors.affiliation: Shanghai University of Chinese Medicine, 1200 Cailun Road, Shanghai 201203, People's Republic of China.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.affiliation: Department of Biochemistry and Molecular Medicine, Comprehensive Cancer Center, University of California at Davis, 4645 2nd Avenue, Sacramento 95817, California.
authors.forename: Hongwu
authors.initials: H
authors.lastname: Chen
authors.affiliation: First Affiliated Hospital, Guangzhou University of Chinese Medicine, 16 Jichang Road, Guangzhou 510405, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: He
authors.affiliation: Department of Biochemistry and Molecular Medicine, Comprehensive Cancer Center, University of California at Davis, 4645 2nd Avenue, Sacramento 95817, California.
authors.forename: Junjian
authors.initials: J
authors.lastname: Wang
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001859:Bone Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D003474:Curcumin / Q000494:pharmacology*; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism*; D012516:Osteosarcoma / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D012334:RNA, Neoplasm / Q000378:metabolism*; D011960:Receptors, Estrogen / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*; D000097983:ERRalpha Estrogen-Related Receptor
chemical_list: D003474:Curcumin; D035683:MicroRNAs; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm; D011960:Receptors, Estrogen; D000097983:ERRalpha Estrogen-Related Receptor; C531888:MIRN125 microRNA, human
keywords: CURCUMIN; ESTROGEN-RELATED RECEPTOR ALPHA; OSTEOSARCOMA; U2OS CELLS; miR-125a