title: Lanthanum induced B-to-Z transition in self-assembled Y-shaped branched DNA structure.
abstract: Controlled conversion of right-handed B-DNA to left-handed Z-DNA is one of the greatest conformational transitions in biology. Recently, the B-Z transition has been explored from nanotechnological points of view and used as the driving machinery of many nanomechanical devices. Using a combination of CD spectroscopy, fluorescence spectroscopy, and PAGE, we demonstrate that low concentration of lanthanum chloride can mediate B-to-Z transition in self-assembled Y-shaped branched DNA (bDNA) structure. The transition is sensitive to the sequence and structure of the bDNA. Thermal melting and competitive dye binding experiments suggest that La(3+) ions are loaded to the major and minor grooves of DNA and stabilize the Z-conformation. Our studies also show that EDTA and EtBr play an active role in reversing the transition from Z-to-B DNA.
authors.affiliation: DNA Nanotechnology &Application Laboratory, CSIR-Institute of Minerals &Materials Technology, Bhubaneswar 751 013, India.
authors.forename: Ashok K
authors.initials: AK
authors.lastname: Nayak
authors.affiliation: DNA Nanotechnology &Application Laboratory, CSIR-Institute of Minerals &Materials Technology, Bhubaneswar 751 013, India.
authors.forename: Aseem
authors.initials: A
authors.lastname: Mishra
authors.affiliation: DNA Nanotechnology &Application Laboratory, CSIR-Institute of Minerals &Materials Technology, Bhubaneswar 751 013, India.
authors.forename: Bhabani S
authors.initials: BS
authors.lastname: Jena
authors.affiliation: DNA Nanotechnology &Application Laboratory, CSIR-Institute of Minerals &Materials Technology, Bhubaneswar 751 013, India.
authors.forename: Barada K
authors.initials: BK
authors.lastname: Mishra
authors.affiliation: DNA Nanotechnology &Application Laboratory, CSIR-Institute of Minerals &Materials Technology, Bhubaneswar 751 013, India.
authors.forename: Umakanta
authors.initials: U
authors.lastname: Subudhi
mesh_terms: D059371:DNA, B-Form / Q000737:chemistry*; D043542:DNA, Z-Form / Q000737:chemistry*; D004492:Edetic Acid / Q000737:chemistry; D004996:Ethidium / Q000737:chemistry; D007811:Lanthanum / Q000737:chemistry*
chemical_list: D059371:DNA, B-Form; D043542:DNA, Z-Form; C028521:lanthanum chloride; D007811:Lanthanum; D004492:Edetic Acid; D004996:Ethidium