title: Reduced Virulence of Azoxystrobin-Resistant Zymoseptoria tritici Populations in Greenhouse Assays.
abstract: The development of resistance to multiple fungicide classes is currently limiting disease management options for many pathogens, while the discovery of new fungicide classes may become less frequent. In light of this, more research is needed to quantify virulence trade-offs of fungicide resistance in order to more fully understand the implications of fungicide resistance on pathogen fitness. The purpose of this study was to measure the virulence of azoxystrobin-resistant and -sensitive Zymoseptoria tritici populations collected from North and South Willamette Valley, Oregon, in 2012 and 2015. Inoculum mixtures of known fungicide-resistant phenotypes were used to simulate natural field conditions, where multiple genotypes exist and interact in close proximity. Six greenhouse inoculations were conducted over 2 years, and virulence of the isolate mixtures was evaluated in planta. We considered virulence to be "the degree of pathology caused by the organism" and visually estimated the percent area of leaf necrosis as a measure of virulence. In greenhouse conditions, a consistent association of reduced virulence with azoxystrobin-resistant Z. tritici isolate mixtures was observed. North Willamette Valley and South Willamette Valley populations did not differ in virulence.
authors.affiliation: Department of Botany and Plant Pathology, Oregon State University, 2082 Cordley Hall, 2701 SW Campus Way, Corvallis 97331.
authors.forename: Christina H
authors.initials: CH
authors.lastname: Hagerty
authors.affiliation: Department of Botany and Plant Pathology, Oregon State University, 2082 Cordley Hall, 2701 SW Campus Way, Corvallis 97331.
authors.forename: Christopher C
authors.initials: CC
authors.lastname: Mundt
mesh_terms: D001203:Ascomycota / Q000187:drug effects* / Q000472:pathogenicity; D025141:Drug Resistance, Fungal; D004780:Environment, Controlled; D005659:Fungicides, Industrial / Q000494:pharmacology; D008689:Methacrylates / Q000494:pharmacology*; D010935:Plant Diseases / Q000382:microbiology*; D011743:Pyrimidines / Q000494:pharmacology*; D000073739:Strobilurins; D014908:Triticum / Q000382:microbiology*; D014774:Virulence
chemical_list: D005659:Fungicides, Industrial; D008689:Methacrylates; D011743:Pyrimidines; D000073739:Strobilurins; C087670:azoxystrobin