title: Electrochemiluminescence (ECL) immunosensor for detection of Francisella tularensis on screen-printed gold electrode array.
abstract: An electrochemiluminescence (ECL) immunosensor for the rapid detection of the Francisella tularensis pathogen using whole antibodies or antibody fragments as capture biomolecule is described. A sandwich immunoassay was used with either lipopolysaccharide (LPS) or the whole inactivated bacterial cell (LVS) as a target, while Ru(bpy)3 (2+)-encapsulated silicate nanoparticles were linked to the secondary antibody and used as ECL labels. The assay was performed in a fluidic chip housed in a custom-built black box incorporating electronics, optics and fluidics. The obtained limit of detection for LPS was 0.4 ng/mL, while for the LVS it was 70 and 45 bacteria/mL when the capturing molecule was the whole antibody and the antibody F(ab) fragment, respectively.
authors.affiliation: Departament d'Enginyeria Quimica, Universitat Rovira i Virgili, Avinguda Països Catalans 26, 43007, Tarragona, Spain. annamaria.spehar@gmail.com.
authors.forename: Anna-Maria
authors.initials: AM
authors.lastname: Spehar-Délèze
authors.affiliation: Institute of Bacterial Infections and Zoonoses, Friedrich-Loeffer-Institut, Naumburger Str. 96A, 07743, Jena, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Julich
authors.affiliation: Fraunhofer ICT-IMM, Carl-Zeiss-Strasse 18-20, 55129, Mainz, Germany.
authors.forename: Rainer
authors.initials: R
authors.lastname: Gransee
authors.affiliation: Institute of Bacterial Infections and Zoonoses, Friedrich-Loeffer-Institut, Naumburger Str. 96A, 07743, Jena, Germany.
authors.forename: Herbert
authors.initials: H
authors.lastname: Tomaso
authors.affiliation: Departament d'Enginyeria Quimica, Universitat Rovira i Virgili, Avinguda Països Catalans 26, 43007, Tarragona, Spain.
authors.forename: Samuel B
authors.initials: SB
authors.lastname: Dulay
authors.affiliation: Departament d'Enginyeria Quimica, Universitat Rovira i Virgili, Avinguda Països Catalans 26, 43007, Tarragona, Spain. ciara.osullivan@urv.cat.|Institució Catalana de Recerca i Estudis Avancats, Passeig Lluís Companys, 23, 08010, Barcelona, Spain. ciara.osullivan@urv.cat.
authors.forename: Ciara K
authors.initials: CK
authors.lastname: O'Sullivan
mesh_terms: D000907:Antibodies, Bacterial / Q000737:chemistry*; D055910:Antibodies, Immobilized / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004566:Electrodes; D004867:Equipment Design; D005604:Francisella tularensis / Q000302:isolation & purification*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000295:instrumentation*; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D009942:Organometallic Compounds / Q000737:chemistry; D017640:Silicates / Q000737:chemistry; D014406:Tularemia / Q000175:diagnosis / Q000382:microbiology
chemical_list: D000907:Antibodies, Bacterial; D055910:Antibodies, Immobilized; D009942:Organometallic Compounds; D017640:Silicates; C415768:tris(2,2'-bipyridyl)ruthenium(II); D006046:Gold
keywords: Antibody fragment; Biowarfare agent; Electrochemiluminescence; Immunoassay; Screen-printed gold array