title: PCR-fingerprint profiles of mitochondrial and genomic DNA extracted from Fetus cervi using different extraction methods.
abstract: The use of Fetus cervi, which is derived from the embryo and placenta of Cervus Nippon Temminck or Cervs elaphus Linnaeus, has been documented for a long time in China. There are abundant species of deer worldwide. Those recorded by China Pharmacopeia (2010 edition) from all the species were either authentic or adulterants/counterfeits. Identification of their origins or authenticity became a key in the preparation of the authentic products. The traditional SDS alkaline lysis and salt-outing methods were modified to extract mt DNA and genomic DNA from fresh and dry Fetus cervi in addition to Fetus from false animals, respectively. A set of primers were designed by bioinformatics to target the intra-and inter-variation. The mt DNA and genomic DNA extracted from Fetus cervi using the two methods meet the requirement for authenticity. Extraction of mt DNA by SDS alkaline lysis is more practical and accurate than extraction of genomic DNA by salt-outing method. There were differences in length and number of segments amplified by PCR between mt DNA from authentic Fetus cervi and false animals Fetus. The distinctive PCR-fingerprint patterns can distinguish the Fetus cervi from adulterants and counterfeit animal Fetus.
authors.affiliation: a School of Laboratory Medicine , Beihua University , Jilin , China.
authors.forename: Jinxia
authors.initials: J
authors.lastname: Ai
authors.affiliation: a School of Laboratory Medicine , Beihua University , Jilin , China.
authors.forename: Xuesong
authors.initials: X
authors.lastname: Wang
authors.affiliation: a School of Laboratory Medicine , Beihua University , Jilin , China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Gao
authors.affiliation: a School of Laboratory Medicine , Beihua University , Jilin , China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xia
authors.affiliation: a School of Laboratory Medicine , Beihua University , Jilin , China.
authors.forename: Mingcheng
authors.initials: M
authors.lastname: Li
authors.affiliation: b School of Pharmacy , Beihua University , Jilin , China.
authors.forename: Guangxin
authors.initials: G
authors.lastname: Yuan
authors.affiliation: a School of Laboratory Medicine , Beihua University , Jilin , China.
authors.forename: Jiamu
authors.initials: J
authors.lastname: Niu
authors.affiliation: c Jilin Leibo Scientific Co. Ltd , Jilin , China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000235:genetics; D005591:Chemical Fractionation / Q000379:methods*; D004247:DNA / Q000302:isolation & purification*; D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D003670:Deer / Q000196:embryology / Q000235:genetics*; D005260:Female; D005333:Fetus; D016678:Genome; D054629:Genome, Mitochondrial; D010920:Placenta; D016133:Polymerase Chain Reaction; D011247:Pregnancy
chemical_list: D004247:DNA
keywords: Authenticity; Fetus cervi; PCR-fingerprint; mitochondrial DNA