title: The relationship between plasma and urinary 8-hydroxy-2-deoxyguanosine biomarkers measured by liquid chromatography tandem mass spectrometry.
abstract: Although 8-hydroxy-2-deoxyguanosine (8-OHdG) is a widely used promising biomarker of DNA damage, there are concerns about which tissues or body fluids should be sampled. The objective of this study is to evaluate the correlation of DNA oxidative damage biomarkers, 8-OHdG, between blood and urine and risk factors associated with 8OHdG. The study population was recruited from a baseline survey of a worksite lifestyle study including 92 office workers aged 23 to 60 years. A self-administered questionnaire was used to collect information on personal characteristics. The plasma and urinary 8-OHdG was measured by liquid chromatography tandem mass spectrometry (LC-MS/MS). In linear regression, a positive relation was found (p < 0.01) between the log-transformed plasma and urinary 8-OHdG levels adjusted for gender, age, BMI, and smoking status. Our findings showed that age, gender and smoking were significantly associated with plasma 8-OHdG, but not with urinary 8-OHdG. Our results suggest that there is a positive relation between the biomarkers of plasma (steady state DNA damage) and urinary 8-OHdG (total DNA damage). However, the plasma 8-OHdG is more sensitive than urinary 8-OHdG to detect increased oxidative damages induced by risk factors.
authors.affiliation: Division of Family Medicine, Department of Family and Community Medicine, Tri-Service General Hospital and School of Medicine, National Defense Medical Center, Taipei, Taiwan, Republic of China.
authors.forename: Chung-Ching
authors.initials: CC
authors.lastname: Wang
authors.affiliation: Division of Family Medicine, Department of Family and Community Medicine, Tri-Service General Hospital and School of Medicine, National Defense Medical Center, Taipei, Taiwan, Republic of China.
authors.forename: Wei-Liang
authors.initials: WL
authors.lastname: Chen
authors.affiliation: Department of Pediatrics, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, Republic of China.|Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, Taiwan, Republic of China.
authors.forename: Chien-Ming
authors.initials: CM
authors.lastname: Lin
authors.affiliation: Department of Public Health, National Defence Medical Center, Taipei, Taiwan, Republic of China.
authors.forename: Ching-Huang
authors.initials: CH
authors.lastname: Lai
authors.affiliation: Division of Gastroenterology and Hepatology, Department of Internal Medicine, Taichung Armed Forces General Hospital, Taiping, Taichung, Taiwan, Republic of China.
authors.forename: Ching-Hui
authors.initials: CH
authors.lastname: Loh
authors.affiliation: Graduate Institute of Medical Sciences, Chang Jung Christian University, Tainan, Taiwan, Republic of China.
authors.forename: Hong-I
authors.initials: HI
authors.lastname: Chen
authors.affiliation: Department of Public Health, National Defence Medical Center, Taipei, Taiwan, Republic of China. shliou@nhri.org.tw.|National Institute of Environmental Health Sciences, National Health Research Institutes, 35 Keyan Road, Zhunan Town, Miaoli County, 35053, Taiwan, Republic of China. shliou@nhri.org.tw.
authors.forename: Saou-Hsing
authors.initials: SH
authors.lastname: Liou
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D015415:Biomarkers / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine; D005260:Female; D006801:Humans; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D012907:Smoking / Q000009:adverse effects; D053719:Tandem Mass Spectrometry / Q000379:methods; D055815:Young Adult
chemical_list: D015415:Biomarkers; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine
keywords: 8-hydroxydeoxyguanosine; LC-MS/MS; Plasma; Urine