title: Critical Role of the Disintegrin Metalloprotease ADAM-like Decysin-1 [ADAMDEC1] for Intestinal Immunity and Inflammation.
abstract: OBJECTIVE
  ADAM [A Disintegrin And Metalloproteinase] is a family of peptidase proteins which have diverse roles in tissue homeostasis and immunity. Here, we study ADAM-like DECysin-1 [ADAMDEC1] a unique member of the ADAM family. ADAMDEC1 expression is restricted to the macrophage/dendritic cell populations of the gastrointestinal tract and secondary lymphoid tissue. The biological function of ADAMDEC1 is unknown but it has been hypothesised to play a role in immunity. The identification of reduced ADAMDEC1 expression in Crohn's disease patients has provided evidence of a potential role in bowel inflammation.
  
  
  METHODS
  Adamdec1-/-mice were exposed to dextran sodium sulphate or infected orally with Citrobacter rodentium or Salmonella typhimurium. The clinical response was monitored.
  
  
  RESULTS
  The loss of Adamdec1 rendered mice more susceptible to the induction of bacterial and chemical induced colitis, as evidenced by increased neutrophil infiltration, greater IL-6 and IL-1β secretion, more weight loss and increased mortality. In the absence of Adamdec1, greater numbers of Citrobacter rodentium were found in the spleen, suggestive of a breakdown in mucosal immunity which resulted in bacteraemia.
  
  
  CONCLUSIONS
  In summary, ADAMDEC1 protects the bowel from chemical and bacterial insults, failure of which may predispose to Crohn's disease.
authors.affiliation: Division of Medicine, University College London, London, UK.
authors.forename: Nuala R
authors.initials: NR
authors.lastname: O'Shea
authors.affiliation: Division of Medicine, University College London, London, UK.
authors.forename: Thean S
authors.initials: TS
authors.lastname: Chew
authors.affiliation: Division of Medicine, University College London, London, UK.
authors.forename: Jenny
authors.initials: J
authors.lastname: Dunne
authors.affiliation: Division of Medicine, University College London, London, UK.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Marnane
authors.affiliation: Division of Medicine, University College London, London, UK.
authors.forename: Bahman
authors.initials: B
authors.lastname: Nedjat-Shokouhi
authors.affiliation: Division of Medicine, University College London, London, UK.
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Smith
authors.affiliation: Department of Gastroenterology, University College London Hospital, UK.
authors.forename: Stuart L
authors.initials: SL
authors.lastname: Bloom
authors.affiliation: Division of Medicine, University College London, London, UK.|Microbial Diseases, Eastman Dental Institute, University College London, London, UK.
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Smith
authors.affiliation: Division of Medicine, University College London, London, UK.
authors.forename: Anthony W
authors.initials: AW
authors.lastname: Segal
mesh_terms: D051722:ADAM Proteins / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D044082:Citrobacter rodentium; D003092:Colitis / Q000276:immunology / Q000503:physiopathology*; D003106:Colon / Q000276:immunology / Q000503:physiopathology; D016264:Dextran Sulfate / Q000494:pharmacology; D004195:Disease Models, Animal; D004756:Enterobacteriaceae Infections / Q000150:complications; D007413:Intestinal Mucosa / Q000276:immunology / Q000503:physiopathology; D051379:Mice; D008810:Mice, Inbred C57BL; D012481:Salmonella Infections, Animal / Q000150:complications; D012486:Salmonella typhimurium
chemical_list: D016264:Dextran Sulfate; D051722:ADAM Proteins; C000619702:Adamdec1 protein, mouse; C108169:decysin
keywords: Citrobacter rodentium; DSS; colitis