title: Cholinesterase in porcine saliva: Analytical characterization and behavior after experimental stress.
abstract: The purpose of this study was to measure and characterize the enzyme cholinesterase (ChE) in porcine saliva, as well as to evaluate its behavior in experimental stressful conditions. The results of ChE characterization by using different substrates and the selective inhibitors ethopropazine and physostigmine showed that the main enzyme existing in porcine saliva was butyrylcholinesterase (BChE). An automated assay using butyrylthiocholine iodide as substrate was validated providing adequate reproducibility, linearity results and limit of detection. Salivary ChE was measured using the validated assay in two models of acute stress: twenty pigs stressed for 2min with a nasal snare and other twenty pigs subjected to a short-term road transport. Salivary ChE significantly increased after restraint and transport stress in pigs, as well as the ChE to total protein ratio. In conclusion, BChE is the predominant isoenzyme in porcine saliva, it can be measured by the fast, simple and automated method described in this paper and it increases in the models of stress used in this study.
authors.affiliation: Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Campus of Excellence Mare Nostrum, University of Murcia, 30100 Espinardo, Murcia, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: Tecles
authors.affiliation: Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Campus of Excellence Mare Nostrum, University of Murcia, 30100 Espinardo, Murcia, Spain.
authors.forename: Damián
authors.initials: D
authors.lastname: Escribano
authors.affiliation: Animal Production Department, Campus of Excellence Mare Nostrum, University of Murcia, 30100 Espinardo, Murcia, Spain.
authors.forename: Silvia
authors.initials: S
authors.lastname: Martínez-Miró
authors.affiliation: Animal Production Department, Campus of Excellence Mare Nostrum, University of Murcia, 30100 Espinardo, Murcia, Spain.
authors.forename: Fuensanta
authors.initials: F
authors.lastname: Hernández
authors.affiliation: Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Campus of Excellence Mare Nostrum, University of Murcia, 30100 Espinardo, Murcia, Spain.
authors.forename: María Dolores
authors.initials: MD
authors.lastname: Contreras
authors.affiliation: Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Campus of Excellence Mare Nostrum, University of Murcia, 30100 Espinardo, Murcia, Spain. Electronic address: jjceron@um.es.
authors.forename: José Joaquín
authors.initials: JJ
authors.lastname: Cerón
mesh_terms: D000827:Animal Welfare; D000818:Animals; D002091:Butyrylcholinesterase / Q000378:metabolism*; D053379:Chromogranin A / Q000378:metabolism*; D015203:Reproducibility of Results; D012463:Saliva / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods / Q000662:veterinary*; D013312:Stress, Physiological; D034421:Sus scrofa / Q000378:metabolism*
chemical_list: D053379:Chromogranin A; D002091:Butyrylcholinesterase
keywords: Cholinesterase; Inhibitors; Pigs; Saliva; Stress; Substrates