title: Regulation of plasma lipid homeostasis by hepatic lipoprotein lipase in adult mice.
abstract: LPL is a pivotal rate-limiting enzyme to catalyze the hydrolysis of TG in circulation, and plays a critical role in regulating lipid metabolism. However, little attention has been paid to LPL in the adult liver due to its relatively low expression. Here we show that endogenous hepatic LPL plays an important physiological role in plasma lipid homeostasis in adult mice. We generated a mouse model with the Lpl gene specifically ablated in hepatocytes with the Cre/LoxP approach, and found that specific deletion of hepatic Lpl resulted in a significant decrease in plasma LPL contents and activity. As a result, the postprandial TG clearance was markedly impaired, and plasma TG and cholesterol levels were significantly elevated. However, deficiency of hepatic Lpl did not change the liver TG and cholesterol contents or glucose homeostasis. Taken together, our study reveals that hepatic LPL is involved in the regulation of plasma LPL activity and lipid homeostasis.
authors.affiliation: Department of Pathophysiology Second Military Medical University, Shanghai 200433, China Obesity and Diabetes Research Center, Second Military Medical University, Shanghai 200433, China.
authors.forename: Gan
authors.initials: G
authors.lastname: Liu
authors.affiliation: Department of Pathophysiology Second Military Medical University, Shanghai 200433, China.
authors.forename: Jun-Nan
authors.initials: JN
authors.lastname: Xu
authors.affiliation: Department of Pathophysiology Second Military Medical University, Shanghai 200433, China Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenchou Medical University School of Laboratory Medicine and Life Sciences, Wenchou, Zhejiang 325035, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Liu
authors.affiliation: Department of Pathophysiology Second Military Medical University, Shanghai 200433, China.
authors.forename: Qingli
authors.initials: Q
authors.lastname: Ding
authors.affiliation: Department of Pathophysiology Second Military Medical University, Shanghai 200433, China Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenchou Medical University School of Laboratory Medicine and Life Sciences, Wenchou, Zhejiang 325035, China.
authors.forename: Meng-Na
authors.initials: MN
authors.lastname: Liu
authors.affiliation: Obesity and Diabetes Research Center, Second Military Medical University, Shanghai 200433, China Department of Endocrinology, Changhai Hospital, Shanghai 200433, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Chen
authors.affiliation: Department of Pathophysiology Second Military Medical University, Shanghai 200433, China Department of Endocrinology, Second Affiliated Hospital, Wenchou Medical University, Wenchou, Zhejiang 325000, China.
authors.forename: Mengdi
authors.initials: M
authors.lastname: Fan
authors.affiliation: Department of Pathophysiology Second Military Medical University, Shanghai 200433, China Obesity and Diabetes Research Center, Second Military Medical University, Shanghai 200433, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Endocrinology, Second Affiliated Hospital, Wenchou Medical University, Wenchou, Zhejiang 325000, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zheng
authors.affiliation: Obesity and Diabetes Research Center, Second Military Medical University, Shanghai 200433, China Department of Endocrinology, Changhai Hospital, Shanghai 200433, China.
authors.forename: Da-Jin
authors.initials: DJ
authors.lastname: Zou
authors.affiliation: Key Laboratory of Laboratory Medicine, Ministry of Education of China, Wenchou Medical University School of Laboratory Medicine and Life Sciences, Wenchou, Zhejiang 325035, China wzhang@smmu.edu.cn jxlu313@163.com.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Lyu
authors.affiliation: Department of Pathophysiology Second Military Medical University, Shanghai 200433, China Obesity and Diabetes Research Center, Second Military Medical University, Shanghai 200433, China wzhang@smmu.edu.cn jxlu313@163.com.
authors.forename: Weiping J
authors.identifier: 0000-0003-4727-2380
authors.initials: WJ
authors.lastname: Zhang
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism / Q000473:pathology; D000818:Animals; D002784:Cholesterol / Q000097:blood; D006706:Homeostasis; D006801:Humans; D015228:Hypertriglyceridemia / Q000097:blood / Q000235:genetics* / Q000473:pathology; D008055:Lipids / Q000097:blood*; D008071:Lipoprotein Lipase / Q000097:blood / Q000235:genetics*; D008099:Liver / Q000201:enzymology* / Q000473:pathology; D051379:Mice; D018345:Mice, Knockout; D019518:Postprandial Period; D014280:Triglycerides / Q000097:blood
chemical_list: D008055:Lipids; D014280:Triglycerides; D002784:Cholesterol; D008071:Lipoprotein Lipase
keywords: hypertriglyceridemia; knockout mice; lipid metabolism; liver