title: Using Single-Cell Amperometry To Reveal How Cisplatin Treatment Modulates the Release of Catecholamine Transmitters during Exocytosis.
abstract: The pretreatment of cultured pheochromocytoma (PC12) cells with cis-diamminedichloroplatinum (cisplatin), an anti-cancer drug, influences the exocytotic ability of the cells in a dose-dependent manner. Low concentrations of cisplatin stimulate catecholamine release whereas high concentrations inhibit it. Single-cell amperometry reflects that 2 μm cisplatin treatment increases the frequency of exocytotic events and reduces their duration, whereas 100 μm cisplatin treatment decreases the frequency of exocytotic events and increases their duration. Furthermore, the stability of the initial fusion pore that is formed in the lipid membrane during exocytosis is also regulated differentially by different cisplatin concentrations. This study thus suggests that cisplatin influences exocytosis by multiple mechanisms.
authors.affiliation: Department of Chemistry and Molecular Biology, University of Gothenburg, Kemivägen 10, 41296, Gothenburg, Sweden.
authors.forename: Xianchan
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Kemivägen 10, 41296, Gothenburg, Sweden.
authors.forename: Johan
authors.initials: J
authors.lastname: Dunevall
authors.affiliation: Department of Chemistry and Molecular Biology, University of Gothenburg, Kemivägen 10, 41296, Gothenburg, Sweden. andrew.ewing@chem.gu.se, andrewe@chalmers.se.|Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Kemivägen 10, 41296, Gothenburg, Sweden. andrew.ewing@chem.gu.se, andrewe@chalmers.se.
authors.forename: Andrew G
authors.identifier: 0000-0002-2084-0133
authors.initials: AG
authors.lastname: Ewing
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002395:Catecholamines / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D002945:Cisplatin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005089:Exocytosis / Q000187:drug effects*; D018377:Neurotransmitter Agents / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D016716:PC12 Cells; D051381:Rats; D059010:Single-Cell Analysis; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D002395:Catecholamines; D018377:Neurotransmitter Agents; D002945:Cisplatin
keywords: amperometry; chemotherapy; cisplatin; exocytosis