title: Cortical gene expression: prognostic value for seizure outcome following temporal lobectomy and amygdalohippocampectomy.
abstract: Whole genome analyses were performed to test the hypothesis that temporal cortical gene expression differs between epilepsy patients rendered seizure-free versus non-seizure-free following anterior temporal lobectomy with amygdalohippocampectomy (ATL/AH). Twenty four patients underwent ATL/AH to treat medically intractable seizures of temporal lobe origin (mean age 35.5 years, mean follow-up 42.2 months); they were then dichotomized into seizure-free and non-seizure-free groups. Tissue RNA was isolated from the lateral temporal cortex and gene expression analysis was performed. Whole genome data were analyzed for prognostic value for seizure-free outcome following ATL/AH by logistic regression. Genes that could distinguish seizure outcome groups were identified based on providing an accuracy of >0.90 judging by area under the receiver operating characteristic curve, AUC, with a P value of the slope coefficient of <0.05. Four genes and seven RNA probes were with prognostic value for post-operative seizure-free outcome. Gene expression associated with seizure-free outcome included relative down-regulation of zinc finger protein 852 (ZNF852), CUB domain-containing protein 2 (CDCP2), proline-rich transmembrane protein 1 (PRRT1), hypothetical LOC440200 (FLJ41170), RNA probe 8047763, RNA probe 8126238, RNA probe 8113489, RNA probe 8092883, RNA probe 7935228, RNA probe 806293, and RNA probe 8104131. This study describes the predictive value of temporal cortical gene expression for seizure-free outcome after ATL/AH. Four genes and seven RNA probes were found to predict post-operative seizure-free outcome. Future prospective investigation of these genes and probes in human brain tissue and blood could establish new biomarkers predictive of seizure outcome following ATL/AH.
authors.affiliation: College of Nursing, University of Arizona, 1305 N Martin Ave, PO Box 210203, Tucson, AZ, 85721, USA. mgallek@email.arizona.edu.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Gallek
authors.affiliation: College of Medicine, Department of Surgery, Division of Neurosurgery, University of Arizona, Tucson, AZ, USA.
authors.forename: Jesse
authors.initials: J
authors.lastname: Skoch
authors.affiliation: Department of Neurosurgery, Brigham and Women's Hospital, Boston, MA, USA.
authors.forename: Tracy
authors.initials: T
authors.lastname: Ansay
authors.affiliation: College of Medicine, Department of Neurosurgery, University of Illinois at Chicago, Chicago, IL, USA.
authors.forename: Mandana
authors.initials: M
authors.lastname: Behbahani
authors.affiliation: Informatics/Bioinformatics Shared Services, Arizona Cancer Center, University of Arizona, Tucson, AZ, USA.
authors.forename: David
authors.initials: D
authors.lastname: Mount
authors.affiliation: Informatics/Bioinformatics Shared Services, Arizona Cancer Center, University of Arizona, Tucson, AZ, USA.
authors.forename: Ann
authors.initials: A
authors.lastname: Manziello
authors.affiliation: College of Medicine, Department of Surgery, University of Arizona, Tucson, AZ, USA.
authors.forename: Marlys
authors.initials: M
authors.lastname: Witte
authors.affiliation: College of Medicine, Department of Surgery, University of Arizona, Tucson, AZ, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Bernas
authors.affiliation: Department of Neurology, College of Medicine, University of Arizona, Tucson, AZ, USA.
authors.forename: David M
authors.initials: DM
authors.lastname: Labiner
authors.affiliation: College of Medicine, Department of Surgery, Division of Neurosurgery, University of Arizona, Tucson, AZ, USA.
authors.forename: Martin E
authors.initials: ME
authors.lastname: Weinand
mesh_terms: D000293:Adolescent; D000328:Adult; D000679:Amygdala / Q000601:surgery*; D038481:Anterior Temporal Lobectomy; D002648:Child; D004827:Epilepsy / Q000175:diagnosis / Q000235:genetics* / Q000601:surgery*; D005260:Female; D015870:Gene Expression; D006624:Hippocampus / Q000601:surgery*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D012313:RNA / Q000235:genetics; D013702:Temporal Lobe / Q000378:metabolism* / Q000601:surgery; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: D012313:RNA
keywords: Amygdalohippocampectomy; Gene expression; Neurosurgical genomics; Prognostic factor; Temporal lobectomy