title: San Huang Shel Shin Tang beta-cyclodextrin complex augmented the hepatoprotective effects against carbon tetrachloride-induced acute hepatotoxicity in rats.
abstract: BACKGROUND
  San Huang Shel Shin Tang (SHSST) is a traditional herbal decoction used as a hepato-protective agent and is composed of Rheum officinale Baill, Scutellaria baicalnsis Geprgi and Coptis chinensis Franch (2:1:1 w/w). Beta-cyclodextrin (β-CD) modification may potentially increase the solubility and spectral properties of SHSST.
  
  
  METHODS
  In this research, the hepato-protective effects of unmodified SHSST, β-CD modified SHSST complex (SHSSTc) and silymarin were evaluated in carbon tetrachloride (CCl4) induced acute hepatotoxicity in rats.
  
  
  RESULTS
  SHHSTc (40 mg/kg/day) and silymarin (100 mg/kg/day) both decreased the CCl4-induced cirrhosis pathway-related transforming growth factor beta (TGF-β) and apoptosis pathway-related caspase-8 protein expressions, but SHSST (40 mg/kg/day) did not reduce TGF-β and caspase-8 significantly . Moreover, SHHSTc (40 mg/kg/day) enhanced the activation of insulin-like growth factor 1 receptor (IGF1R) mediated survival pathway than the silymarin (100 mg/kg/day) to protect the liver from damage induced by CCl4.
  
  
  CONCLUSIONS
  β-CD modification promotes hepato-protective effects of SHSST and reduces the required-dosage of the SHSST.
authors.affiliation: Department of Pathology, Changhua Christian Hospital, Changhua, 50006, Taiwan.|Department of Medical Technology, Jen-Teh Junior College of Medicine, Nursing and Management, Miaoli, 35664, Taiwan.
authors.forename: Yu-Lan
authors.initials: YL
authors.lastname: Yeh
authors.affiliation: Graduate Institute of Basic Medical Science, China Medical University and Hospital, No. 91, Hsueh-Shih Road, Taichung, 40402, Taiwan.
authors.forename: Wei-Jen
authors.initials: WJ
authors.lastname: Ting
authors.affiliation: Department of Biological Science and Technology, China Medical University, Taichung, 40402, Taiwan.
authors.forename: Wei-Wen
authors.initials: WW
authors.lastname: Kuo
authors.affiliation: Division of Colorectal Surgery, Mackay Memorial Hospital, Taipei, 10449, Taiwan.
authors.forename: Hsi-Hsien
authors.initials: HH
authors.lastname: Hsu
authors.affiliation: Department of Pathology, Changhua Christian Hospital, Changhua, 50006, Taiwan.|Department of Medical Technology, Jen-Teh Junior College of Medicine, Nursing and Management, Miaoli, 35664, Taiwan.
authors.forename: Yueh-Min
authors.initials: YM
authors.lastname: Lin
authors.affiliation: Department of Nursing, MeiHo University, Pingtung, 91201, Taiwan.
authors.forename: Chia-Yao
authors.initials: CY
authors.lastname: Shen
authors.affiliation: Institute of Cellular and System Medicine, National Health Research Institutes, Zhunan Town, 35053, Taiwan.
authors.forename: Chung-Ho
authors.initials: CH
authors.lastname: Chang
authors.affiliation: Department of Biotechnology, Bharathiar University, Coimbatore, 641 046, India.
authors.forename: Viswanadha Vijaya
authors.initials: VV
authors.lastname: Padma
authors.affiliation: School of Chinese Medicine, China Medical University, No. 91 Hsueh-Shih Road, Taichung, 40402, Taiwan. cyhuang@mail.cmu.edu.tw.
authors.forename: Yuhsin
authors.initials: Y
authors.lastname: Tsai
authors.affiliation: Graduate Institute of Basic Medical Science, China Medical University and Hospital, No. 91, Hsueh-Shih Road, Taichung, 40402, Taiwan. yhtsai@mail.cmu.edu.tw.|School of Chinese Medicine, China Medical University, No. 91 Hsueh-Shih Road, Taichung, 40402, Taiwan. yhtsai@mail.cmu.edu.tw.|Department of Health and Nutrition Biotechnology, Asia University, Taichung, 41354, Taiwan. yhtsai@mail.cmu.edu.tw.
authors.forename: Chih-Yang
authors.initials: CY
authors.lastname: Huang
mesh_terms: D000818:Animals; D002251:Carbon Tetrachloride; D056486:Chemical and Drug Induced Liver Injury / Q000517:prevention & control*; D004357:Drug Synergism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D047392:beta-Cyclodextrins / Q000494:pharmacology*
chemical_list: D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D020011:Protective Agents; C501029:San-Huang-Xie-Xin-Tang; D047392:beta-Cyclodextrins; D002251:Carbon Tetrachloride; C031215:betadex
keywords: Beta-cyclodextrin; CCl4; Liver protection; San Huang Shel Shin Tang (SHSST); Silymarin