title: A high-resolution mitochondria-targeting ratiometric fluorescent probe for detection of the endogenous hypochlorous acid.
abstract: Hypochlorite anion, one of the biologically important reactive oxygen species, plays an essential role in diverse normal biochemical functions and abnormal pathological processes. Herein, an efficient high-resolution mitochondria-targeting ratiometric fluorescent probe for hypochlorous acid detection has been designed, synthesized and characterized. It is easily synthesized by the condensation reaction (CC) of a 2-(2-hydroxyphenyl) quinazolin-4(3H)-one fluorophore and a cyanine group (mitochondria-targeting), which made the whole molecular a large Stokes shift (210nm) and the two well-resolved emission peaks separated by 140nm. As a result, it is considered as a good candidate for high resolution hypochlorous acid imaging in live cells. The ratiometric fluorescent probe exhibited outstanding features of high sensitivity, high selectivity, rapid response time (within 50s), and excellent mitochondria-targeting ability. Moreover, the probe can also be successfully applied to imaging endogenously hypochlorous acid in the mitochondria of living cells with low cytotoxicity, and high resolution.
authors.affiliation: College of Packaging and Materials Engineering, Hunan University of Technology, Hunan 412007, PR China; Molecular Sciences and Biomedicine Laboratory, State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University, Changsha 410082, China; Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, PR China. Electronic address: zhouly0817@163.com.
authors.forename: Liyi
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Molecular Sciences and Biomedicine Laboratory, State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University, Changsha 410082, China.
authors.forename: Dan-Qing
authors.initials: DQ
authors.lastname: Lu
authors.affiliation: Molecular Sciences and Biomedicine Laboratory, State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University, Changsha 410082, China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: College of Packaging and Materials Engineering, Hunan University of Technology, Hunan 412007, PR China.
authors.forename: Shunqin
authors.initials: S
authors.lastname: Hu
authors.affiliation: College of Packaging and Materials Engineering, Hunan University of Technology, Hunan 412007, PR China.
authors.forename: Haifei
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, PR China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Sun
authors.affiliation: Molecular Sciences and Biomedicine Laboratory, State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University, Changsha 410082, China.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Zhang
mesh_terms: D002232:Carbocyanines / Q000737:chemistry*; D002460:Cell Line; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D006997:Hypochlorous Acid / Q000032:analysis*; D008856:Microscopy, Fluorescence / Q000379:methods; D008928:Mitochondria / Q000737:chemistry* / Q000648:ultrastructure; D061848:Optical Imaging / Q000379:methods; D052999:Quinazolinones / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D002232:Carbocyanines; D005456:Fluorescent Dyes; D052999:Quinazolinones; C011203:cyanine dye 2; D006997:Hypochlorous Acid
keywords: Fluorescent probe; HPQ-Cy(2); Hypochlorous acid; Mitochondria; Ratiometric imaging