title: The isolation and the characterization of two polysaccharides from the branch bark of mulberry (Morus alba L.).
abstract: Two water-soluble polysaccharides termed MBBP-1 and MBBP-2 were isolated from the branches of the mulberry tree (Morus alba L.) using hot water extraction and purified on Anion-exchange DEAE52-cellulose and Sephadex G-100 column. MBBP-1 was shown to be composed of rhamnose, xylose, arabinose, mannose, glucose and galactose in the molar ratio of 4.53:2.49:4.38:4.67:17.85:5.88. MBBP-2 was composed of rhamnose, xylose, arabinose, mannose, glucose, galactose and galacturonic acid in the molar ratio of 26.85:13.8:3.14:4.4:6.1:3.19:4.9. Their structural characteristics were further investigated by FI-IR spectroscopy, Smith degradation, methylation analysis and NMR spectroscopy. Based on the data obtained, MBBP-1 had a backbone mainly consisting of (1 → 3)-linked glucose. MBBP-2 had a backbone mainly consisting of (1 → 3)-linked rhamnose and (1 → 2, 4)-linked xylose. Antioxidant assays indicated that antioxidant activities of MBBP-2 were significantly stronger than those of MBBP-1, and this was likely in relation to the different content of 8.2 % galacturonic acid in MBBP-2.
authors.affiliation: Applied Biological Department, Medical College of Soochow University, Suzhou, 215123, People's Republic of China.|The State Engineering Laboratory of Modern Silk, Soochow University, Suzhou, 215123, People's Republic of China.
authors.forename: Fan
authors.initials: F
authors.lastname: Qiu
authors.affiliation: Applied Biological Department, Medical College of Soochow University, Suzhou, 215123, People's Republic of China.|The State Engineering Laboratory of Modern Silk, Soochow University, Suzhou, 215123, People's Republic of China.
authors.forename: Tian-Zhen
authors.initials: TZ
authors.lastname: He
authors.affiliation: Applied Biological Department, Medical College of Soochow University, Suzhou, 215123, People's Republic of China. sericult@suda.edu.cn.|The State Engineering Laboratory of Modern Silk, Soochow University, Suzhou, 215123, People's Republic of China. sericult@suda.edu.cn.
authors.forename: Yu-Qing
authors.initials: YQ
authors.lastname: Zhang
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D031625:Morus; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D011134:Polysaccharides
keywords: Antioxidant activities; Chemical structure; Mulberry branch bark; Polysaccharide