title: Nature-Derived Aloe Vera Gel Blended Silk Fibroin Film Scaffolds for Cornea Endothelial Cell Regeneration and Transplantation.
abstract: The goal of this study was to fabricate an appropriate replacement for cadaveric corneas to overcome a shortage of cadaveric corneas for transplantation. In this study, we fabricated transparent ultrathin film scaffolds with nature-derived aloe vera (AV) gel and silk fibroin (SF) for corneal endothelial cells (CECs). The scaffolds were subjected to analysis of transparency and contact angle using field emission scanning electron microscopy (FESEM) and Fourier transform infrared spectroscopy to determine their physical and chemical properties. FESEM images revealed that the critical morphology of CECs was formed on the AV gel in the blend with SF rather than in the scaffold with SF alone. The cell proliferation, phenotype, and specific gene marker expressions for CECs were determined by MTT assays, immunofluorescence, and reverse transcription polymerase chain reactions. Incorporation of a small amount of AV gel increased the cell viability and maintained its functions well. The scaffolds were easily handled for transplantation into rabbit eyes with small incisions and examined by their transparency after transplantation and histological staining. The scaffolds attached to the surface of the corneal stroma and integrated with surrounding corneal tissue without a significant inflammatory reaction. These results indicate that AV blended SF film scaffolds might be a suitable substitute for alternative corneal grafts for transplantation.
authors.affiliation: Department of BIN Fusion Technology, Department of Polymer Nano Science & Technology, and Polymer BIN Research Center, Chonbuk National University , Deokjin-gu, Jeonju 561-756, Republic of Korea.
authors.forename: Do Kyung
authors.initials: do K
authors.lastname: Kim
authors.affiliation: Department of BIN Fusion Technology, Department of Polymer Nano Science & Technology, and Polymer BIN Research Center, Chonbuk National University , Deokjin-gu, Jeonju 561-756, Republic of Korea.
authors.forename: Bo Ra
authors.initials: BR
authors.lastname: Sim
authors.affiliation: Department of BIN Fusion Technology, Department of Polymer Nano Science & Technology, and Polymer BIN Research Center, Chonbuk National University , Deokjin-gu, Jeonju 561-756, Republic of Korea.
authors.forename: Gilson
authors.initials: G
authors.lastname: Khang
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D017690:Cell Transplantation; D003315:Cornea / Q000166:cytology / Q000502:physiology; D042783:Endothelial Cells / Q000166:cytology; D005349:Fibroins / Q000737:chemistry*; D028321:Plant Preparations / Q000737:chemistry*; D011817:Rabbits; D012038:Regeneration; D047011:Silk / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: C586178:Aloe vera gel; D028321:Plant Preparations; D047011:Silk; D005349:Fibroins
keywords: aloe vera gel; cornea endothelial cells; scaffold; silk fibroin; tissue engineering