title: Ascorbic acid accumulates as a defense response to Turnip mosaic virus in resistant Brassica rapa cultivars.
abstract: We initially observed that Brassica rapa cultivars containing the Turnip mosaic virus (TuMV) resistance gene, Rnt1-1, accumulated a high level of endogenous ascorbic acid (AS) and dehydroascobic acid (DHA) when infected with TuMV. We here hypothesized a possible contribution of an elevated level of AS+DHA (TAA) to the Rnt1-1-mediated resistance, and conducted a series of experiments using B. rapa and Arabidopsis plants. The application of l-galactose (the key substrate in AS synthesis) to a susceptible cultivar could increase the TAA level ~2-fold, and simultaneously lead to some degree of enhanced viral resistance. To confirm some positive correlation between TAA levels and viral resistance, we analyzed two Arabidopsis knockout mutants (ao and vtc1) in the AS pathways; the TAA levels were significantly increased and decreased in ao and vtc1 plants, respectively. While the ao plants showed enhanced resistance to TuMV, vtc1 plants were more susceptible than the control, supporting our hypothesis. When we analyzed the expression profiles of the genes involved in the AS pathways upon TuMV infection, we found that the observed TAA increase was mainly brought about by the reduction of AS oxidation and activation of AS recycling. We then investigated the secondary signals that regulate endogenous TAA levels in response to viral infection, and found that jasmonic acid (JA) might play an important role in TAA accumulation. In conclusion, we reason that the elevated TAA accumulation in B. rapa plants would be at least partly mediated by the JA-dependent signaling pathway and may significantly contribute to viral resistance.
authors.affiliation: Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Ayaka
authors.initials: A
authors.lastname: Fujiwara
authors.affiliation: Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Satoko
authors.initials: S
authors.lastname: Togawa
authors.affiliation: Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Hikawa
authors.affiliation: Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Matsuura
authors.affiliation: Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Chikara
authors.initials: C
authors.lastname: Masuta
authors.affiliation: Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan yoshi@abs.agr.hokudai.ac.jp.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Inukai
mesh_terms: D017360:Arabidopsis / Q000502:physiology; D001205:Ascorbic Acid / Q000378:metabolism / Q000502:physiology*; D029741:Brassica rapa / Q000235:genetics / Q000502:physiology* / Q000821:virology; D060467:Disease Resistance / Q000235:genetics / Q000502:physiology*; D017343:Genes, Plant / Q000235:genetics / Q000502:physiology; D017800:Potyvirus / Q000472:pathogenicity*
chemical_list: D001205:Ascorbic Acid
keywords: Ascorbate oxidase; Brassica rapa; Turnip mosaic virus.; ascorbate peroxidase; ascorbic acid; dehydroascorbate reductase; jasmonic acid