title: See-Through Technology for Biological Tissue: 3-Dimensional Visualization of Macromolecules.
abstract: Tissue clearing technology is currently one of the fastest growing fields in biomedical sciences. Tissue clearing techniques have become a powerful approach to understand further the structural information of intact biological tissues. Moreover, technological improvements in tissue clearing and optics allowed the visualization of neural network in the whole brain tissue with subcellular resolution. Here, we described an overview of various tissue-clearing techniques, with focus on the tissue-hydrogel mediated clearing methods, and discussed the main advantages and limitations of transparent tissue for clinical diagnosis.
authors.affiliation: Department of Anatomy and Division of Brain Korea 21 Plus Program for Biomedical Science, Korea University College of Medicine, Seoul, Korea.
authors.forename: Eunsoo
authors.identifier: 0000-0002-1973-0930
authors.initials: E
authors.lastname: Lee
authors.affiliation: Department of Anatomy and Division of Brain Korea 21 Plus Program for Biomedical Science, Korea University College of Medicine, Seoul, Korea.
authors.forename: Hyun Jung
authors.identifier: 0000-0002-9520-1109
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Department of Anatomy and Division of Brain Korea 21 Plus Program for Biomedical Science, Korea University College of Medicine, Seoul, Korea.
authors.forename: Woong
authors.identifier: 0000-0003-1792-4894
authors.initials: W
authors.lastname: Sun
keywords: Imaging, Three-Dimensional; Immunolabeling; Molecular Diagnosis; Neuroscience; Optical Tissue Clearing