title: Crystal Structure of Cytochrome P450 (CYP105P2) from Streptomyces peucetius and Its Conformational Changes in Response to Substrate Binding.
abstract: Cytochrome P450 monooxygenases (CYP, EC 1.14.14.1) belong to a large family of enzymes that catalyze the hydroxylation of various substrates. Here, we present the crystal structure of CYP105P2 isolated from Streptomyces peucetius ATCC27952 at a 2.1 Å resolution. The structure shows the presence of a pseudo-ligand molecule in the active site, which was co-purified fortuitously and is presumed to be a biphenyl derivative. Comparison with previously determined substrate-bound CYP structures showed that binding of the ligand produces large and distinctive conformational changes in α2-α3, α7-α9, and the C-terminal loop regions. This structural flexibility confirms our previous observation that CYP105P2 can accommodate a broad range of ligands. The structure complexed with a pseudo-ligand provides the first molecular view of CYP105P2-ligand interactions, and it indicates the involvement of hydrophobic residues (Pro82, Ala181, Met187, Leu189, Leu193, and Ile236) in the interactions between hydrophobic ligands and CYP105P2. These results provide useful insights into the structural changes involved in the recognition of different ligands by CYP105P2.
authors.affiliation: Division of Polar Life Sciences, Korea Polar Research Institute, Incheon 406-840, Korea. justay@kopri.re.kr.|Department of Polar Sciences, University of Science and Technology, Incheon 406-840, Korea. justay@kopri.re.kr.
authors.forename: Chang Woo
authors.initials: CW
authors.lastname: Lee
authors.affiliation: Department of BT-Convergent Pharmaceutical Engineering, Sunmoon University, Asansi 336-708, Korea. shadowjhl@empal.com.
authors.forename: Joo-Ho
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of BT-Convergent Pharmaceutical Engineering, Sunmoon University, Asansi 336-708, Korea. rimalhem@gmail.com.
authors.forename: Hemraj
authors.initials: H
authors.lastname: Rimal
authors.affiliation: Division of Polar Life Sciences, Korea Polar Research Institute, Incheon 406-840, Korea. hpark@kopri.re.kr.|Department of Polar Sciences, University of Science and Technology, Incheon 406-840, Korea. hpark@kopri.re.kr.
authors.forename: Hyun
authors.initials: H
authors.lastname: Park
authors.affiliation: Division of Polar Life Sciences, Korea Polar Research Institute, Incheon 406-840, Korea. junhyucklee@kopri.re.kr.|Department of Polar Sciences, University of Science and Technology, Incheon 406-840, Korea. junhyucklee@kopri.re.kr.
authors.forename: Jun Hyuck
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of BT-Convergent Pharmaceutical Engineering, Sunmoon University, Asansi 336-708, Korea. tjoh3782@sunmoon.ac.kr.
authors.forename: Tae-Jin
authors.initials: TJ
authors.lastname: Oh
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry* / Q000378:metabolism; D006860:Hydrogen Bonding; D057927:Hydrophobic and Hydrophilic Interactions; D008024:Ligands; D008958:Models, Molecular; D011485:Protein Binding; D017433:Protein Structure, Secondary; D013302:Streptomyces / Q000737:chemistry / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D008024:Ligands; D003577:Cytochrome P-450 Enzyme System
keywords: Streptomyces peucetius; X-ray crystallography; crystal structure; cytochrome P450