title: Application of a loop-mediated isothermal amplification (LAMP) assay for molecular identification of Trueperella pyogenes isolated from various origins.
abstract: In the present study 28 Trueperella pyogenes strains isolated from various origins could successfully be identified with a newly designed loop-mediated isothermal amplification (LAMP) assay based on gene cpn60 encoding chaperonin. No cross reaction could be observed with control strains representing four species of genus Trueperella and seven species of closely related genus Arcanobacterium. The present cpn60 LAMP assay might allow a reliable and low cost identification of T. pyogenes also in laboratories with less specified equipment.
authors.affiliation: Institute of Food Quality and Food Safety, Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany. Electronic address: amir.abdulmawjood@tiho-hannover.de.
authors.forename: A
authors.initials: A
authors.lastname: Abdulmawjood
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, Schubertstr 81, 35392 Gießen, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Wickhorst
authors.affiliation: Institute of Food Quality and Food Safety, Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany.
authors.forename: O
authors.initials: O
authors.lastname: Hashim
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, Schubertstr 81, 35392 Gießen, Germany.
authors.forename: O
authors.initials: O
authors.lastname: Sammra
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, Schubertstr 81, 35392 Gießen, Germany.
authors.forename: A A
authors.initials: AA
authors.lastname: Hassan
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, Schubertstr 81, 35392 Gießen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Alssahen
authors.affiliation: Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, Schubertstr 81, 35392 Gießen, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Lämmler
authors.affiliation: Institut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität, Frankfurterstr. 85-91, 35392 Gießen, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Prenger-Berninghoff
authors.affiliation: Institute of Food Quality and Food Safety, Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Klein
mesh_terms: D055667:Arcanobacterium / Q000235:genetics* / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D005798:Genes, Bacterial; D057230:Limit of Detection; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*
keywords: LAMP; Loop-mediated isothermal amplification; Real-time fluorometer; Trueperella pyogenes