title: Antibodies and Selection of Monoclonal Antibodies.
abstract: Monoclonal antibodies are universal binding molecules with a high specificity for their target and are indispensable tools in research, diagnostics and therapy. The biotechnological generation of monoclonal antibodies was enabled by the hybridoma technology published in 1975 by Köhler and Milstein. Today monoclonal antibodies are used in a variety of applications as flow cytometry, magnetic cell sorting, immunoassays or therapeutic approaches. First step of the generation process is the immunization of the organism with appropriate antigen. After a positive immune response the spleen cells are isolated and fused with myeloma cells in order to generate stable, long-living antibody-producing cell lines - hybridoma cells. In the subsequent identification step the culture supernatants of all hybridoma cells are screened weekly for the production of the antibody of interest. Hybridoma cells producing the antibody of interest are cloned by limited dilution till a monoclonal hybridoma is found. This is a very time-consuming and laborious process and therefore different selection strategies were developed since 1975 in order to facilitate the generation of monoclonal antibodies. Apart from common automation of pipetting processes and ELISA testing there are some promising approaches to select the right monoclonal antibody very early in the process to reduce time and effort of the generation. In this chapter different selection strategies for antibody-producing hybridoma cells are presented and analysed regarding to their benefits compared to conventional limited dilution technology.
authors.affiliation: Research Group "Antibody Technologies", University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476, Potsdam - Golm, Germany. katja.hanack@uni-potsdam.de.
authors.forename: Katja
authors.initials: K
authors.lastname: Hanack
authors.affiliation: Research Group "Antibody Technologies", University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476, Potsdam - Golm, Germany.
authors.forename: Katrin
authors.initials: K
authors.lastname: Messerschmidt
authors.affiliation: Research Group "Antibody Technologies", University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476, Potsdam - Golm, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Listek
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000302:isolation & purification*; D002469:Cell Separation / Q000379:methods*; D006801:Humans; D006825:Hybridomas; D007114:Immunization; D051379:Mice
chemical_list: D000911:Antibodies, Monoclonal
keywords: B lymphocyte s; ELISA; Hybridoma technology; Monoclonal antibodies; Selection of antibody producing cells