title: Genome-wide association study for sperm membrane integrity in frozen-thawed semen of Holstein-Friesian bulls.
abstract: The aim of the study was to screen the entire bull genome to identify SNP markers and propose candidate genes potentially involved in the variation of sperm membrane integrity in Holstein-Friesian bulls. Two hundred eighty eight bulls kept in one AI center were included in the study. Each bull was genotyped for 54.001 Single Nucleotide Polymorpisms (SNP) by the Illumina BovineSNP50 BeadChip. Commercial straws of frozen-thawed semen were used for the evaluation of sperm plasma membrane integrity (SYBR-14/PI staining) and sperm mitochondrial function (JC1/PI staining). An additive model for Linear Regression Analysis was applied to estimate the effect of SNP marker for sperm membrane integrity (by the use of GoldenHelix SVS7 software). Five significant markers (encompassing 2,2 MB region located on chromosome 6) for SYBR-14/PI were found. Among them one marker-rs41570391 passed Bonferroni correction test. Within approximately 3 Mb genomic region including significant markers three candidate genes: SGMS2 (Sphingomyelin Synthase 2), TET2 (Methylcytosine dioxygenase 2) and GSTCD genes (Gluthatione S-transferase C terminal domain) were proposed as potentially involved in sperm membrane integrity in frozen-thawed semen of Holstein-Friesian bulls.
authors.affiliation: University of Warmia and Mazury, Department of Animal Genetics, ul. M. Oczapowskiego 5, 10-718 Olsztyn, Poland. Electronic address: stachel@uwm.edu.pl.
authors.forename: Stanisław
authors.initials: S
authors.lastname: Kamiński
authors.affiliation: University of Warmia and Mazury, Department of Animal Genetics, ul. M. Oczapowskiego 5, 10-718 Olsztyn, Poland.
authors.forename: Dorota M
authors.initials: DM
authors.lastname: Hering
authors.affiliation: University of Warmia and Mazury, Department of Animal Genetics, ul. M. Oczapowskiego 5, 10-718 Olsztyn, Poland.
authors.forename: Kamil
authors.initials: K
authors.lastname: Oleński
authors.affiliation: University of Warmia and Mazury, Department of Animal Biochemistry and Biotechnology, ul. M. Oczapowskiego 5, 10-718 Olsztyn, Poland.
authors.forename: Marek
authors.initials: M
authors.lastname: Lecewicz
authors.affiliation: University of Warmia and Mazury, Department of Animal Biochemistry and Biotechnology, ul. M. Oczapowskiego 5, 10-718 Olsztyn, Poland.
authors.forename: Władysław
authors.initials: W
authors.lastname: Kordan
mesh_terms: D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000502:physiology*; D015925:Cryopreservation / Q000662:veterinary; D005615:Freezing; D005786:Gene Expression Regulation; D055106:Genome-Wide Association Study / Q000662:veterinary*; D005838:Genotype; D008297:Male; D020641:Polymorphism, Single Nucleotide; D012662:Semen Preservation / Q000662:veterinary*
keywords: Bull; Genome-wide association study; SNP; Sperm membrane