title: Comparative antibacterial activity of topical antiseptic eardrops against methicillin-resistant Staphylococcus aureus and quinolone-resistant Pseudomonas aeruginosa.
abstract: OBJECTIVE
  Aural irrigation using antiseptic solutions can be an effective medical treatment of chronic suppurative otitis media (CSOM) owing to the increasing prevalence of antibiotic-resistant CSOM infections. In the present study, we compared the antimicrobial activities of 100% Burow's solution, 50% Burow's solution, 2% acetic acid, vinegar with water (1:1), and 4% boric acid solution against methicillin-resistant Staphylococcus aureus (MRSA), methicillin-susceptible S. aureus (MSSA), quinolone-resistant Pseudomonas aeruginosa (QRPA), and quinolone-susceptible P. aeruginosa (QSPA) in vitro.
  
  
  METHODS
  We examined the antimicrobial activities of five antiseptic solutions against MRSA, MSSA, QRPA, and QSPA. The antimicrobial activities of the solutions were calculated as a percentage of the surviving microorganisms by dividing the viable count in each antiseptic solution with that in control. The time (D10 value) required for each of the five solutions to inactivate 90% of the microorganism population was also investigated.
  
  
  RESULTS
  Burow's solution exhibited the highest antimicrobial activity and the lowest D10 value against MRSA, MSSA, QRPA, and QSPA, followed by 2% acetic acid, vinegar with water (1:1), and 4% boric acid solution.
  
  
  CONCLUSIONS
  Our results indicate that Burow's solution has the most potent activity against bacteria including antibiotic-resistant strains. Twofold dilution of the solution is recommended to avoid ototoxicity.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Chosun University School of Medicine, Gwangju, South Korea; Division of Natural Medical Science, Chosun University, Gwangju, South Korea.
authors.forename: Cha Kyung
authors.initials: CK
authors.lastname: Youn
authors.affiliation: Department of Laboratory Medicine, Chosun University School of Medicine, Gwangju, South Korea.
authors.forename: Sook-Jin
authors.initials: SJ
authors.lastname: Jang
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Chosun University School of Medicine, Gwangju, South Korea.
authors.forename: Eu-Ri
authors.initials: ER
authors.lastname: Jo
authors.affiliation: Department of Laboratory Medicine, Chosun University School of Medicine, Gwangju, South Korea.
authors.forename: Ji Ae
authors.initials: JA
authors.lastname: Choi
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Chosun University School of Medicine, Gwangju, South Korea.
authors.forename: Ju-Hwan
authors.initials: JH
authors.lastname: Sim
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Chosun University School of Medicine, Gwangju, South Korea. Electronic address: chosi@chosun.ac.kr.
authors.forename: Sung Il
authors.initials: SI
authors.lastname: Cho
mesh_terms: D000085:Acetates / Q000008:administration & dosage / Q000494:pharmacology*; D019342:Acetic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D000287:Administration, Topical; D000891:Anti-Infective Agents, Local / Q000008:administration & dosage / Q000494:pharmacology*; D001888:Boric Acids / Q000008:administration & dosage / Q000494:pharmacology*; D002908:Chronic Disease; D024881:Drug Resistance, Bacterial; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D010035:Otitis Media, Suppurative / Q000382:microbiology; D019999:Pharmaceutical Solutions; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D015363:Quinolones
chemical_list: D000085:Acetates; D000891:Anti-Infective Agents, Local; D001888:Boric Acids; D019999:Pharmaceutical Solutions; D015363:Quinolones; C013396:aluminum acetate; D019342:Acetic Acid; C032688:boric acid
keywords: Anti-infective agents; Methicillin-resistant Staphylococcus aureus; Otitis media; Quinolone-resistant Pseudomonas aeruginosa