title: Jatrophane and ingenane-type diterpenoids from Euphorbia kansui inhibit the LPS-induced NO production in RAW 264.7 cells.
abstract: Bioactivity-guided fractionation of the MeOH extract from the roots of Euphorbia kansui resulted in the isolation of two new jatrophane-type diterpenoids, kanesulones A (1) and B (2), together with six known jatrophane-type diterpenoids (3-8) and ten known ingenane-type diterpenoids (9-18). Their chemical structures were elucidated on the basis of spectroscopic data interpretation, especially 1D and 2D NMR such as HMQC, HMBC, COSY and NOESY, and HRESIMS data as well as CD analysis. Compounds 1-8 and 11-18 exhibited the inhibitory effects on LPS-induced nitric oxide production with IC50 values ranging from 0.7 to 46.5μM in RAW 264.7 macrophages.
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju 28644, Republic of Korea.
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju 28644, Republic of Korea.
authors.forename: Qinghao
authors.initials: Q
authors.lastname: Jin
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju 28644, Republic of Korea.
authors.forename: Hari
authors.initials: H
authors.lastname: Jang
authors.affiliation: College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Republic of Korea.
authors.forename: Dongho
authors.initials: D
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju 28644, Republic of Korea.
authors.forename: Sang Bae
authors.initials: SB
authors.lastname: Han
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju 28644, Republic of Korea.
authors.forename: Youngsoo
authors.initials: Y
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju 28644, Republic of Korea.
authors.forename: Jin Tae
authors.initials: JT
authors.lastname: Hong
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju 28644, Republic of Korea.
authors.forename: Mi Kyeong
authors.initials: MK
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju 28644, Republic of Korea. Electronic address: byhwang@chungbuk.ac.kr.
authors.forename: Bang Yeon
authors.initials: BY
authors.lastname: Hwang
mesh_terms: D000818:Animals; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D028481:Euphorbia / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D000067996:RAW 264.7 Cells; D013329:Structure-Activity Relationship
chemical_list: D004224:Diterpenes; D008070:Lipopolysaccharides; C530110:ingenane; C530345:jatrophane; D009569:Nitric Oxide
keywords: Diterpenoid; Euphorbia kansui; Euphorbiaceae; Nitric oxide production inhibitor