title: Turning the corner in fertility: high DNA integrity of boundary-following sperm.
abstract: We present a passive microfluidic sperm selection strategy that collects motile sperm based on their preference to follow boundaries and turn corners. Clinical assessment of selected human sperm from the device revealed a strong correlation between high DNA integrity and the tendency for sperm to follow boundaries. Human sperm with preference to follow boundaries on the left- or right-hand sides have higher (>51%) DNA integrity than straight swimmers and significantly higher (>67%) DNA integrity than sperm in raw semen. Boundary following behaviour offers a strategy to selecting sperm with the highest DNA integrity to improve the success rate of assisted reproduction.
authors.affiliation: Department of Mechanical and Industrial Engineering, University of Toronto, 5 King's College Rd., Toronto, ON M5S 3G8, Canada. sinton@mie.utoronto.ca.
authors.forename: Lise
authors.initials: L
authors.lastname: Eamer
authors.forename: Marion
authors.initials: M
authors.lastname: Vollmer
authors.forename: Reza
authors.initials: R
authors.lastname: Nosrati
authors.forename: Maria C
authors.initials: MC
authors.lastname: San Gabriel
authors.forename: Krista
authors.initials: K
authors.lastname: Zeidan
authors.forename: Armand
authors.initials: A
authors.lastname: Zini
authors.forename: David
authors.initials: D
authors.lastname: Sinton
mesh_terms: D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods*; D004247:DNA / Q000032:analysis; D004867:Equipment Design; D006801:Humans; D056656:Lab-On-A-Chip Devices; D008297:Male; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*
chemical_list: D004247:DNA