title: Molecular Evaluation of Genetic Diversity in Wild-Type Mastic Tree (Pistacia lentiscus L.).
abstract: In this study, the patterns of genetic variation and phylogenetic relationships of mastic tree (Pistacia lentiscus L.) genotypes including 12 males and 12 females were evaluated using SSR, RAPD, ISSR, and ITS markers yielding 40, 703, 929 alleles, and 260-292 base pairs for ITS1 region, respectively. The average number of alleles produced from SSR, RAPD, and ISSR primers were 5.7, 14, and 18, respectively. The grouping pattern obtained from Bayesian clustering method based on each marker dataset was produced. Principal component analyses (PCA) of molecular data was investigated and neighbor joining dendrograms were subsequently created. Overall, the results indicated that ISSR and RAPD markers were the most powerful to differentiate the genotypes in comparison with other types of molecular markers used in this study. The ISSR results indicated that male and female genotypes were distinctly separated from each other. In this frame, M9 (Alaçatı) and M10 (Mesta Sakız Adası-Chios) were the closest genotypes and while F11 (Seferihisar) and F12 (Bornova/Gökdere) genotypes fall into same cluster and showing closer genetic relation. The RAPD pattern indicated that M8 (Urla) and M10 (Mesta Sakız Adası-Chios), and F10 (Mesta Sakız Adası-Chios) and F11 (Seferihisar) genotypes were the closest male and female genotypes, respectively.
authors.affiliation: Department of Bioengineering, Faculty of Engineering, Marmara University, 34722, Istanbul, Turkey.
authors.forename: Alimu
authors.initials: A
authors.lastname: Abuduli
authors.affiliation: Department of Biology, Faculty of Arts and Sciences, Marmara University, 34722, Istanbul, Turkey.
authors.forename: Yıldız
authors.initials: Y
authors.lastname: Aydin
authors.affiliation: Department of Bioengineering, Faculty of Engineering and Architecture, Kafkas University, 36100, Kars, Turkey.
authors.forename: Muhammet
authors.initials: M
authors.lastname: Sakiroglu
authors.affiliation: Department of Biology, Faculty of Science, University of Dicle, Diyarbakir, 21280, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Onay
authors.affiliation: Department of Horticulture, Agricultural Faculty, Ataturk University, 25240, Erzurum, Turkey.
authors.forename: Sezai
authors.initials: S
authors.lastname: Ercisli
authors.affiliation: Department of Bioengineering, Faculty of Engineering, Marmara University, 34722, Istanbul, Turkey. ahu.uncuoglu@marmara.edu.tr.
authors.forename: Ahu Altinkut
authors.initials: AA
authors.lastname: Uncuoglu
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods; D018744:DNA, Plant / Q000235:genetics*; D005787:Gene Frequency; D005819:Genetic Markers; D010802:Phylogeny; D027927:Pistacia / Q000235:genetics*; D011110:Polymorphism, Genetic; D025341:Principal Component Analysis
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers
keywords: DNA markers; Genetic diversity; Phylogenetic relationship; Population structure; Principle component analysis