title: Performance of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Aspergillus, Scedosporium, and Fusarium spp. in the Australian Clinical Setting.
abstract: We developed an Australian database for the identification of Aspergillus, Scedosporium, and Fusarium species (n = 28) by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). In a challenge against 117 isolates, species identification significantly improved when the in-house-built database was combined with the Bruker Filamentous Fungi Library compared with that for the Bruker library alone (Aspergillus, 93% versus 69%; Fusarium, 84% versus 42%; and Scedosporium, 94% versus 18%, respectively).
authors.affiliation: Centre for Infectious Diseases and Microbiology Laboratory Services, Institute for Clinical Pathology and Medical Research, Westmead Hospital, The University of Sydney, Westmead, New South Wales, Australia.
authors.forename: Sue
authors.initials: S
authors.lastname: Sleiman
authors.affiliation: Centre for Infectious Diseases and Microbiology Laboratory Services, Institute for Clinical Pathology and Medical Research, Westmead Hospital, The University of Sydney, Westmead, New South Wales, Australia.
authors.forename: Catriona L
authors.initials: CL
authors.lastname: Halliday
authors.affiliation: Westmead Institute for Medical Research, University of Sydney, Westmead, New South Wales, Australia.
authors.forename: Belinda
authors.initials: B
authors.lastname: Chapman
authors.affiliation: Centre for Infectious Diseases and Microbiology Laboratory Services, Institute for Clinical Pathology and Medical Research, Westmead Hospital, The University of Sydney, Westmead, New South Wales, Australia.
authors.forename: Mitchell
authors.initials: M
authors.lastname: Brown
authors.affiliation: Centre for Infectious Diseases and Microbiology Laboratory Services, Institute for Clinical Pathology and Medical Research, Westmead Hospital, The University of Sydney, Westmead, New South Wales, Australia.
authors.forename: Joanne
authors.initials: J
authors.lastname: Nitschke
authors.affiliation: Microbiology Service, Department of Laboratory Medicine, Clinical Centre, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Anna F
authors.initials: AF
authors.lastname: Lau
authors.affiliation: Centre for Infectious Diseases and Microbiology Laboratory Services, Institute for Clinical Pathology and Medical Research, Westmead Hospital, The University of Sydney, Westmead, New South Wales, Australia Marie Bashir Institute for Infectious Diseases and Biosecurity, University of Sydney, New South Wales, Australia sharon.chen@health.nsw.gov.au.
authors.forename: Sharon C-A
authors.initials: SC
authors.lastname: Chen
mesh_terms: D001230:Aspergillus / Q000737:chemistry / Q000302:isolation & purification*; D001315:Australia; D005658:Fungi; D005670:Fusarium / Q000737:chemistry / Q000302:isolation & purification*; D008828:Microbiological Techniques / Q000379:methods*; D009181:Mycoses / Q000175:diagnosis* / Q000382:microbiology*; D021681:Scedosporium / Q000737:chemistry / Q000302:isolation & purification*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*