title: pH-Degradable Mannosylated Nanogels for Dendritic Cell Targeting.
abstract: We report on the design of glycosylated nanogels via core-cross-linking of amphiphilic non-water-soluble block copolymers composed of an acetylated glycosylated block and a pentafluorophenyl (PFP) activated ester block prepared by reversible addition-fragmentation (RAFT) polymerization. Self-assembly, pH-sensitive core-cross-linking, and removal of remaining PFP esters and protecting groups are achieved in one pot and yield fully hydrated sub-100 nm nanogels. Using cell subsets that exhibit high and low expression of the mannose receptor (MR) under conditions that suppress active endocytosis, we show that mannosylated but not galactosylated nanogels can efficiently target the MR that is expressed on the cell surface of primary dendritic cells (DCs). These nanogels hold promise for immunological applications involving DCs and macrophage subsets.
authors.forename: Ruben
authors.initials: R
authors.lastname: De Coen
authors.forename: Nane
authors.initials: N
authors.lastname: Vanparijs
authors.forename: Martijn D P
authors.initials: MD
authors.lastname: Risseeuw
authors.forename: Lien
authors.initials: L
authors.lastname: Lybaert
authors.forename: Benoit
authors.initials: B
authors.lastname: Louage
authors.forename: Stefaan
authors.initials: S
authors.lastname: De Koker
authors.forename: Vimal
authors.initials: V
authors.lastname: Kumar
authors.forename: Johan
authors.initials: J
authors.lastname: Grooten
authors.affiliation: Department of Chemistry, University of Cincinnati , Cincinnati, Ohio 45221, United States.
authors.forename: Leeanne
authors.initials: L
authors.lastname: Taylor
authors.affiliation: Department of Chemistry, University of Cincinnati , Cincinnati, Ohio 45221, United States.
authors.forename: Neil
authors.initials: N
authors.lastname: Ayres
authors.forename: Serge
authors.initials: S
authors.lastname: Van Calenbergh
authors.forename: Lutz
authors.initials: L
authors.lastname: Nuhn
authors.forename: Bruno G
authors.initials: BG
authors.lastname: De Geest
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D037181:Lectins, C-Type / Q000378:metabolism; D008358:Mannose / Q000737:chemistry* / Q000378:metabolism; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins / Q000378:metabolism; D051379:Mice; D000080385:Nanogels; D011092:Polyethylene Glycols / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*; D058105:Polymerization; D011108:Polymers / Q000737:chemistry*; D011956:Receptors, Cell Surface / Q000378:metabolism
chemical_list: D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D000080385:Nanogels; D011108:Polymers; D011956:Receptors, Cell Surface; C413692:polyethylene glycol polyethyleneimine nanogel; D011092:Polyethylene Glycols; D011094:Polyethyleneimine; D008358:Mannose