title: SNAI1 promotes the development of HCC through the enhancement of proliferation and inhibition of apoptosis.
abstract: SNAI1, a zinc-finger transcription factor, plays an important role in the induction of epithelial-mesenchymal transition (EMT) in various cancers. However, the possible functions of SNAI1 in the proliferation and apoptosis of hepatocellular carcinoma have not been clearly identified. In this study, we investigated the effects and mechanisms of SNAI1 in the proliferation and apoptosis of hepatocellular carcinoma using clinical samples and cell lines. We found that SNAI1 is highly expressed in the tissues of liver cancer compared with adjacent nontumor tissues. SNAI1 is also highly expressed in the hepatoma cell lines HepG2, SMMC-7721, and BEL-7402 compared with the human normal liver cell line L02. We also observed that SNAI1 expression was correlated with distal metastasis, incomplete tumor capsule formation, and histological differentiation in hepatocellular carcinoma (HCC). Moreover, we demonstrated that knockdown of SNAI1 via lentiviral vectors of RNAi against SNAI inhibited cell proliferation by inducing G1 arrest, which was accompanied by the downregulation of cyclin D1 but not that of cyclin A. In addition, knockdown of SNAI1 promoted apoptosis by decreasing the expression of Bcl-2. In conclusion, our findings revealed that SNAI1 is involved in the development of hepatocellular carcinoma via regulating the growth and apoptosis of tumor cells.
authors.affiliation: Central Laboratory Shandong Provincial Hospital Affiliated to Shandong University Jinan Shandong China.
authors.forename: Jianni
authors.initials: J
authors.lastname: Qi
authors.affiliation: Department of Gastroenterology Shandong Provincial Hospital Affiliated to Shandong University Jinan Shandong China.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.affiliation: Department of Emergency Shandong Provincial Hospital Affiliated to Shandong University Jinan Shandong China.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Bian
authors.affiliation: Department of Gastroenterology Shandong Provincial Hospital Affiliated to Shandong University Jinan Shandong China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratory Shandong Provincial Hospital Affiliated to Shandong University Jinan Shandong China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Ju
authors.affiliation: Department of Gastroenterology Shandong Provincial Hospital Affiliated to Shandong University Jinan Shandong China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Gao
authors.affiliation: Department of Gastroenterology Shandong Provincial Hospital Affiliated to Shandong University Jinan Shandong China.
authors.forename: Jingran
authors.initials: J
authors.lastname: Su
authors.affiliation: Department of Gastroenterology Shandong Provincial Hospital Affiliated to Shandong University Jinan Shandong China.
authors.forename: Wanhua
authors.initials: W
authors.lastname: Ren
authors.affiliation: Department of Gastroenterology Shandong Provincial Hospital Affiliated to Shandong University Jinan Shandong China.
authors.forename: Chengyong
authors.initials: C
authors.lastname: Qin
keywords: Bcl‐2; Cyclin D1; RNAi; SNAI1; hepatocellular carcinoma