title: Chemotherapeutic effect of Berberis integerrima hydroalcoholic extract on colon cancer development in the 1,2-dimethyl hydrazine rat model.
abstract: The aim of this study was to investigate the efficacy of a Berberis integerrima hydroalcoholic extract as a chemotherapeutic agent in colon carcinogenesis in the rat induced by 1,2-dimethyl hydrazine (DMH). Male Wistar rats were divided into five groups: a negative control group without DMH treatment; a control group injected DMH (20 mg/kg b.w); two groups receiving B. integerrima extract (50 and 100 mg/kg b.w), concomitant with injected DMH, as chemotherapeutic groups; a positive control group receiving 5-fluorouracil (5-FU) along with DMH. The effects of the extracts were determined by assessment of hepatic malondialdehyde (MDA), glutathione (GSH), ferric reducing ability of plasma (FRAP), and the activities of hepatic glutathione S-transferase and cytochrome P450 (GST and CYP450). Additionally, colon tissues were assessed for colonic β-catenin and histopathological analysis. In DMH-treated rats, the extracts partially normalized the levels of FRAP, CYP450, β-catenin, and GST. Likewise, formation of aberrant crypt foci (ACF) in colon tissue of DMH-treated was reduced by the extracts. Thus, the extracts possess chemotherapeutic activity against colon carcinogenesis.
authors.forename: Mohammad R Mohammadi
authors.initials: MR
authors.lastname: Malayeri
authors.forename: Abolfazl
authors.initials: A
authors.lastname: Dadkhah
authors.forename: Faezeh
authors.initials: F
authors.lastname: Fatemi
authors.forename: Salome
authors.initials: S
authors.lastname: Dini
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Torabi
authors.forename: Mohammad M
authors.initials: MM
authors.lastname: Tavajjoh
authors.forename: Javad
authors.initials: J
authors.lastname: Rabiei
mesh_terms: D019813:1,2-Dimethylhydrazine; D058739:Aberrant Crypt Foci / Q000188:drug therapy / Q000378:metabolism; D000818:Animals; D028142:Berberis / Q000737:chemistry*; D002273:Carcinogens; D003106:Colon / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004195:Disease Models, Animal; D000431:Ethanol / Q000737:chemistry; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D017208:Rats, Wistar; D016896:Treatment Outcome; D014867:Water / Q000737:chemistry; D051176:beta Catenin / Q000378:metabolism
chemical_list: D002273:Carcinogens; D010936:Plant Extracts; D051176:beta Catenin; D014867:Water; D000431:Ethanol; D008315:Malondialdehyde; D003577:Cytochrome P-450 Enzyme System; D005982:Glutathione Transferase; D005978:Glutathione; D019813:1,2-Dimethylhydrazine