title: Purification and Adoptive Transfer of Group 3 Gut Innate Lymphoid Cells.
abstract: Recent studies have identified several related but distinct innate lymphoid cells (ILCs) populations that are relatively enriched in the intestinal mucosal and protect the host from various infections. Among ILCs, group 3 ILCs (ILC3s) produce lymphotoxin and IL-22, and play important roles in the development of the immune system and host-bacteria interactions. Here, we describe methods for the isolation and purification of ILC3s from the mouse intestine, and the adoptive transfer of purified ILC3s into recipient mice.
authors.affiliation: Tsinghua University School of Medicine, Medical Science Building D317, Beijing, 100084, China. guoxiaohuan@biomed.tsinghua.edu.cn.
authors.forename: Xiaohuan
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of Pathology and Committee on Immunology, The University of Chicago, Chicago, IL, 60637, USA.
authors.forename: Kevin
authors.initials: K
authors.lastname: Muite
authors.affiliation: Department of Pathology and Committee on Immunology, The University of Chicago, Chicago, IL, 60637, USA.
authors.forename: Joanna
authors.initials: J
authors.lastname: Wroblewska
authors.affiliation: Department of Pathology and Committee on Immunology, The University of Chicago, Chicago, IL, 60637, USA.
authors.forename: Yang-Xin
authors.initials: YX
authors.lastname: Fu
mesh_terms: D019264:Adoptive Transfer / Q000379:methods*; D000818:Animals; D002469:Cell Separation; D006706:Homeostasis; D007113:Immunity, Innate; D020381:Interleukin-17 / Q000378:metabolism; D017710:Lymphocyte Transfusion / Q000379:methods*; D008214:Lymphocytes / Q000166:cytology* / Q000276:immunology; D008233:Lymphotoxin-alpha / Q000378:metabolism; D051379:Mice
chemical_list: D020381:Interleukin-17; D008233:Lymphotoxin-alpha
keywords: Adoptive transfer; Group 3 Innate lymphoid cells; Intestine; Purification; Rag1