title: Ecological study on antimicrobial-resistant zoonotic bacteria transmitted by flies in cattle farms.
abstract: Flies were qualitatively and quantitatively monitored on both livestock animals and the surrounding environment to investigate their role as a potential carrier for antimicrobial-resistant bacteria of zoonotic importance in cattle farms. This was done by the use of visual observations and animal photography; meanwhile, in the surrounding environment, flies were collected using sticky cards and then microscopically identified. Representative fly samples were cultured for bacterial isolation, biochemical identification, and then tested against common 12 antibiotics. The total average of dipterous flies in examined farms was 400.42 ± 6.2. Culicoides biting midges were the most common existing species (70.01 %) followed by house flies, stable flies, and mosquitoes (18.31, 7.74, and 3.91 %, respectively) at X (2) = 9.0, P < 0.05. The most predominant bacterial isolates were Escherichia coli (22.6 %), Staphylococcus aureus and Enterobacter (17.3 % each), coagulase-negative Staphylococci (CNS) (14.7 %), Klebsiella sp. (8 %), Salmonella spp. (6.7 %), and Shigella spp. and Proteus spp. (6.7 % each). The tested bacterial isolates were resistant to variant antibiotics used. S. aureus exhibited 100 % resistance to colistine. However, E. coli revealed 92.9 and 78.6 % resistance against tetracycline and colistine, respectively. Both Salmonella spp. and Shigella spp. were 100 % resistant to penicillin, and Klebsiella sp. had 100 % resistance to tetracycline. In conclusion, Culicoides biting midges and house flies could be considered as a potential carrier for multi-drug-resistant bacteria of zoonotic importance. Furthermore, cows' environment has an essential role in propagation and wide spread of antimicrobial-resistant bacterial pathogens.
authors.affiliation: Department of Hygiene, Management and Zoonoses, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, 62511, Egypt.
authors.forename: Asmaa N
authors.initials: AN
authors.lastname: Mohammed
authors.affiliation: Department of Hygiene, Management and Zoonoses, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, 62511, Egypt.
authors.forename: Gihan K
authors.initials: GK
authors.lastname: Abdel-Latef
authors.affiliation: Department of Hygiene, Management and Zoonoses, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, 62511, Egypt.
authors.forename: Naglaa M
authors.initials: NM
authors.lastname: Abdel-Azeem
authors.affiliation: Department of Parasitology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, 62511, Egypt. eldakley_s71@yahoo.com.
authors.forename: Khaled Mohamed
authors.initials: KM
authors.lastname: El-Dakhly
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects* / Q000302:isolation & purification; D001424:Bacterial Infections / Q000382:microbiology*; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D002519:Ceratopogonidae / Q000382:microbiology; D004175:Diptera / Q000382:microbiology*; D024881:Drug Resistance, Bacterial; D004463:Ecology; D000072480:Farms; D005260:Female; D006793:Houseflies / Q000382:microbiology; D006801:Humans; D007303:Insect Vectors / Q000382:microbiology*; D058751:Livestock; D008826:Microbial Sensitivity Tests; D015047:Zoonoses
chemical_list: D000890:Anti-Infective Agents
keywords: Animal health; Antimicrobial resistance; Environment; Flies; Zoonotic pathogens