title: A subset of neurons controls the permeability of the peritrophic matrix and midgut structure in Drosophila adults.
abstract: The metazoan gut performs multiple physiological functions, including digestion and absorption of nutrients, and also serves as a physical and chemical barrier against ingested pathogens and abrasive particles. Maintenance of these functions and structures is partly controlled by the nervous system, yet the precise roles and mechanisms of the neural control of gut integrity remain to be clarified in Drosophila Here, we screened for GAL4 enhancer-trap strains and labeled a specific subsets of neurons, using Kir2.1 to inhibit their activity. We identified an NP3253 line that is susceptible to oral infection by Gram-negative bacteria. The subset of neurons driven by the NP3253 line includes some of the enteric neurons innervating the anterior midgut, and these flies have a disorganized proventricular structure with high permeability of the peritrophic matrix and epithelial barrier. The findings of the present study indicate that neural control is crucial for maintaining the barrier function of the gut, and provide a route for genetic dissection of the complex brain-gut axis in adults of the model organism Drosophila.
authors.affiliation: Department of Molecular Genetics, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980-8578, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kenmoku
authors.affiliation: Department of Molecular Genetics, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980-8578, Japan Immune Signal Unit, Okinawa Institute of Science and Technology Graduate University, Okinawa, 904-0495, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Ishikawa
authors.affiliation: Department of Molecular Genetics, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980-8578, Japan Division of Neurogenetics, Graduate School of Life Sciences, Tohoku University, Sendai 980-8577, Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Ote
authors.affiliation: Department of Molecular Genetics, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980-8578, Japan Department of Microbiology and Immunology, Keio University School of Medicine, Tokyo 160-8582, Japan Faculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Ishikawa 920-1192, Japan PRESTO, Japan Science and Technology Agency, Tokyo 102-0076, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Kuraishi
authors.affiliation: Department of Molecular Genetics, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980-8578, Japan kurata@mail.pharm.tohoku.ac.jp.
authors.forename: Shoichiro
authors.identifier: 0000-0002-0301-872X
authors.initials: S
authors.lastname: Kurata
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D001424:Bacterial Infections / Q000378:metabolism; D001978:Bromphenol Blue / Q000378:metabolism; D002460:Cell Line; D004064:Digestive System / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000382:microbiology / Q000502:physiology*; D005109:Extracellular Matrix / Q000378:metabolism*; D005247:Feeding Behavior; D005260:Female; D049452:Green Fluorescent Proteins / Q000378:metabolism; D009474:Neurons / Q000502:physiology*; D010539:Permeability; D010641:Phenotype; D016019:Survival Analysis
chemical_list: D001978:Bromphenol Blue; D049452:Green Fluorescent Proteins
keywords: Drosophila; Enteric neurons; Peritrophic matrix