title: Effects of 3G cell phone exposure on the structure and function of the human cytochrome P450 reductase.
abstract: Cell phones increase exposure to radiofrequency (RF) electromagnetic fields (EMFs). Whether EMFs exert specific effects on biological systems remains debatable. This study investigated the effect of cell phone exposure on the structure and function of human NADPH-cytochrome P450 reductase (CPR). CPR plays a key role in the electron transfer to cytochrome P450, which takes part in a wide range of oxidative metabolic reactions in various organisms from microbes to humans. Human CPR was exposed for 60min to 1966-MHz RF inside a transverse electromagnetic cell (TEM-cell) placed in an incubator. The specific absorption rate (SAR) was 5W·kg(-1). Conformation changes have been detected through fluorescent spectroscopy of flavin and tryptophan residues, and investigated through circular dichroism, dynamic light scattering and microelectrophoresis. These showed that CPR was narrowed. By using cytochrome C reductase activity to assess the electron flux through the CPR, the Michaelis Menten constant (Km) and the maximum initial velocity (Vmax) decreased by 22% as compared with controls. This change was due to small changes in the tertiary and secondary structures of the protein at 37°C. The relevance of these findings to an actual RF exposure scenario demands further biochemical and in-vivo confirmation.
authors.affiliation: Sorbonne Universités, Université de Technologie de Compiègne, Laboratoire de Génie Enzymatique et Cellulaire, FRE CNRS 3580, CS60319, 60203 Compiègne Cedex. France.
authors.forename: Shazia
authors.initials: S
authors.lastname: Tanvir
authors.affiliation: Unité de toxicologie expérimentale TOXI-PériTox UMR-I 01, Institut National de l'Environnement Industriel et des RISques (INERIS), Parc ALATA BP2, 60 550, Verneuil-en-Halatte, France; PériTOX, UPJV, Faculté de Médecine, 80 000 Amiens, France.
authors.forename: György
authors.initials: G
authors.lastname: Thuróczy
authors.affiliation: Unité de toxicologie expérimentale TOXI-PériTox UMR-I 01, Institut National de l'Environnement Industriel et des RISques (INERIS), Parc ALATA BP2, 60 550, Verneuil-en-Halatte, France; PériTOX, UPJV, Faculté de Médecine, 80 000 Amiens, France.
authors.forename: Brahim
authors.initials: B
authors.lastname: Selmaoui
authors.affiliation: Université de Picardie Jules Verne, Laboratoire de Glycochimie, des Antimicrobiens et des Agroressources, UMR CNRS 7378, UFR de Pharmacie, 80 037, Amiens, France.
authors.forename: Viviane
authors.initials: V
authors.lastname: Silva Pires Antonietti
authors.affiliation: Université de Picardie Jules Verne, Laboratoire de Glycochimie, des Antimicrobiens et des Agroressources, UMR CNRS 7378, UFR de Pharmacie, 80 037, Amiens, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Sonnet
authors.affiliation: Université de Limoges, CNRS, XLIM, UMR 7252, F-87000, Limoges, France.
authors.forename: Delia
authors.initials: D
authors.lastname: Arnaud-Cormos
authors.affiliation: Université de Limoges, CNRS, XLIM, UMR 7252, F-87000, Limoges, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Lévêque
authors.affiliation: Sorbonne Universités, Université de Technologie de Compiègne, Laboratoire de Génie Enzymatique et Cellulaire, FRE CNRS 3580, CS60319, 60203 Compiègne Cedex. France.
authors.forename: Sylviane
authors.initials: S
authors.lastname: Pulvin
authors.affiliation: Unité de toxicologie expérimentale TOXI-PériTox UMR-I 01, Institut National de l'Environnement Industriel et des RISques (INERIS), Parc ALATA BP2, 60 550, Verneuil-en-Halatte, France; PériTOX, UPJV, Faculté de Médecine, 80 000 Amiens, France. Electronic address: rene.de-seze@ineris.fr.
authors.forename: René
authors.initials: R
authors.lastname: de Seze
mesh_terms: D040421:Cell Phone; D004138:Dinitrocresols / Q000737:chemistry / Q000378:metabolism; D004579:Electron Transport / Q000528:radiation effects; D006801:Humans; D009251:NADPH-Ferrihemoprotein Reductase / Q000737:chemistry* / Q000378:metabolism*; D011846:Radio Waves / Q000009:adverse effects*; D013696:Temperature; D014364:Tryptophan / Q000737:chemistry / Q000378:metabolism
chemical_list: D004138:Dinitrocresols; C024132:4,6-dinitro-o-cresol; D014364:Tryptophan; D009251:NADPH-Ferrihemoprotein Reductase
keywords: Cell phone radiofrequency; Circular dichroism; Cytochrome P450 reductase; Dynamic light scattering; Fluorescence spectroscopy; Zeta potential