title: Analysis of Cell Cycle Status of Murine Hematopoietic Stem Cells.
abstract: Hematopoietic stem cells (HSC) act as paradigmatic tissue-specific adult stem cells. While they are quiescent in steady-state conditions, they enter the cell cycle and proliferate in stress conditions and during tissue regeneration. Therefore, analysis of cell cycle status of HSC is crucial for understanding their biology. However, due to low number of HSC in tissue and need to use many surface markers for their identification, analysis of their cycle status is technically complicated. Here, we presented our simple strategy to analyze cell cycle of strictly defined LKS CD48(-)CD150(+)CD34(-) HSC, together with Ki67 and DAPI staining by flow cytometry.
authors.affiliation: Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland. krzysztof.szade@uj.edu.pl.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Szade
authors.affiliation: Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.
authors.forename: Karolina
authors.initials: K
authors.lastname: Bukowska-Strakova
authors.affiliation: Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Zukowska
authors.affiliation: Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.
authors.forename: Alicja
authors.initials: A
authors.lastname: Jozkowicz
authors.affiliation: Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.
authors.forename: Józef
authors.initials: J
authors.lastname: Dulak
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D002453:Cell Cycle / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics; D002469:Cell Separation / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D051379:Mice; D013312:Stress, Physiological / Q000235:genetics
chemical_list: D015415:Biomarkers
keywords: Activation; Cell cycle phase; Dormancy; Flow cytometry; G0; G1; Hematopoietic stem cells; Ki67; Proliferation; Quiescence