title: The Association Between Low Back Pain and Composition of IgG Glycome.
abstract: Low back pain (LBP) is a common debilitating condition which aetiology and pathogenesis are poorly understood. We carried out a first so far analysis of associations between LBP and plasma IgG N-glycome in a sample of 4511 twins from TwinsUK database assessed for LBP, lumbar disc degeneration (LDD) as its possible cause, and IgG-glycan levels. Using weighted correlation network analysis, we established a correlation between LBP and glycan modules featured by glycans that either promote or block antibody-dependent cell-mediated cytotoxicity (ADCC). The levels of four glycan traits representing two of those modules were statistically significantly different in monozygotic twins discordant for LBP. Also, the trend to higher prevalence of systemic inflammatory disorders was shown for twins with low level of fucosylated glycans and high level of non-fucosylated glycans. Core fucosylation of IgG is a "safety switch" reducing ADCC, thus our results suggest the involvement of ADCC and associated inflammation in pathogenesis of LBP. No correlation between LDD scores and glycans was found assuming that the inflammation may not be a part of LDD. These data provide a new insight into understanding the complex pathophysiology of LBP and suggest glycan levels as a possible biomarker for inflammation-related subtypes of LBP.
authors.affiliation: Department of Twin Research and Genetic Epidemiology, King's College London, London, UK.
authors.forename: Maxim B
authors.initials: MB
authors.lastname: Freidin
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia.
authors.forename: Toma
authors.initials: T
authors.lastname: Keser
authors.affiliation: Genos Glycoscience Research Laboratory, Zagreb, Croatia.
authors.forename: Ivan
authors.initials: I
authors.lastname: Gudelj
authors.affiliation: Genos Glycoscience Research Laboratory, Zagreb, Croatia.
authors.forename: Jerko
authors.initials: J
authors.lastname: Štambuk
authors.affiliation: Department of Twin Research and Genetic Epidemiology, King's College London, London, UK.|Department of Molecular Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia.
authors.forename: Dunja
authors.initials: D
authors.lastname: Vučenović
authors.affiliation: Department of Surgical Science, University of Parma; Anaesthesia Intensive Care and Pain Therapy Service, Parma Hospital, Parma, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Allegri
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia.
authors.forename: Tamara
authors.initials: T
authors.lastname: Pavić
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia.
authors.forename: Mirna
authors.initials: M
authors.lastname: Šimurina
authors.affiliation: Department of Twin Research and Genetic Epidemiology, King's College London, London, UK.
authors.forename: Stella M
authors.initials: SM
authors.lastname: Fabiane
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia.|Genos Glycoscience Research Laboratory, Zagreb, Croatia.
authors.forename: Gordan
authors.initials: G
authors.lastname: Lauc
authors.affiliation: Department of Twin Research and Genetic Epidemiology, King's College London, London, UK.
authors.forename: Frances M K
authors.initials: FM
authors.lastname: Williams
mesh_terms: D000920:Antibody-Dependent Cell Cytotoxicity; D015415:Biomarkers / Q000097:blood; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007249:Inflammation / Q000097:blood; D055959:Intervertebral Disc Degeneration / Q000097:blood / Q000209:etiology; D017116:Low Back Pain / Q000097:blood* / Q000209:etiology; D008297:Male; D008875:Middle Aged; D011134:Polysaccharides / Q000097:blood*; D014430:Twins, Monozygotic
chemical_list: D015415:Biomarkers; D007074:Immunoglobulin G; D011134:Polysaccharides