title: Isolation and analysis of mannose/trehalose/maltose specific lectin from jack bean with antibruchid activity.
abstract: A lectin with insecticidal property against the stored product pest, Callosobruchus maculatus was successfully isolated from the seeds of Canavalia virosa using standard affinity chromatography. The isolated molecule typically behaved like a lectin in its characteristics. It agglutinated indicator red blood cells (RBC) in its native as well as enzyme treated conditions. The enzyme treated RBC types exhibited a very high hemagglutination (HA) titre values and this property of isolated molecule behaved like arcelin, the lectin-like molecules reported from several species of Phaseolus. As a characteristic feature of a lectin, the isolated molecule effectively inhibited the agglutination of indicator RBC types with simple and complex carbohydrates including glycoproteins. This nature of the isolated molecule also relate with characteristic feature of arcelin isoforms in inhibiting HA activity with complex glycoproteins as reported in many studies. Most interestingly, the present study disclosed trehalose as a potent inhibitor of C. virosa lectin. Therefore, feeding insect pests on the lectin like arcelin could serve as antibiosis factor/anti-insect activity. The molecular characteristics of this isolated molecule and its mass studies too revealed its homology with arcelin, arcelin-1, 2 and 6 isoforms of P. vulgaris and lectin from Canavalia cathartica, C. lineata and C. brasiliensis.
authors.affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai, 600025, India.
authors.forename: Sakthivelkumar
authors.initials: S
authors.lastname: Shanmugavel
authors.affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai, 600025, India.
authors.forename: Veeramani
authors.initials: V
authors.lastname: Velayutham
authors.affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai, 600025, India.
authors.forename: Tamilarasan
authors.initials: T
authors.lastname: Kamalanathan
authors.affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai, 600025, India.
authors.forename: Mullainadhan
authors.initials: M
authors.lastname: Periasamy
authors.affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai, 600025, India.
authors.forename: Arumugam
authors.initials: A
authors.lastname: Munusamy
authors.affiliation: Department of Zoology, University of Madras, Guindy Campus, Chennai, 600025, India. Electronic address: janas_09@yahoo.co.in.
authors.forename: Janarthanan
authors.initials: J
authors.lastname: Sundaram
mesh_terms: D000327:Adsorption; D000595:Amino Acid Sequence; D000818:Animals; D001681:Biological Assay; D032449:Canavalia / Q000737:chemistry*; D001517:Coleoptera; D004492:Edetic Acid / Q000737:chemistry; D006384:Hemagglutination / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007306:Insecticides / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D008320:Maltose / Q000378:metabolism*; D037241:Mannose-Binding Lectins / Q000032:analysis* / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology; D055550:Protein Stability; D011817:Rabbits; D012639:Seeds / Q000737:chemistry; D013379:Substrate Specificity; D013696:Temperature; D014199:Trehalose / Q000378:metabolism*
chemical_list: D007306:Insecticides; D037241:Mannose-Binding Lectins; D008320:Maltose; D004492:Edetic Acid; D014199:Trehalose
keywords: Arcelin; Callosobruchus maculatus; Canavalia virosa; Insecticidal lectin