title: Study of the Vapor Phase Over Fusarium Fungi Cultured on Various Substrates.
abstract: The compositions of volatile organic compounds (VOCs) emitted by Fusarium fungi (F. langsethiae, F. sibiricum, F. poae, and F. sporotrichioides) grown on two nutritive substrates: potato sucrose agar (PSA) and autoclaved wheat kernels (WK) were investigated. The culturing of fungi and study of their VOC emissions were performed in chromatographic vials at room temperature (23 - 24 °C) and the VOCs were sampled by a solid-phase microextraction on a 85 μm carboxen/polydimethylsiloxane fiber. GC/MS was performed using a 60-m HP-5 capillary column. Components of the VOC mixture were identified by electron impact mass spectra and chromatographic retention indices (RIs). The most abundant components of the VOC mixture emitted by Fusarium fungi are EtOH, AcOH, (i) BuOH, 3-methylbutan-1-ol, 2-methylbutan-1-ol, ethyl 3-methylbutanoate, terpenes with M 136, sesquiterpenes with M 204 (a total of about 25), and trichodiene. It was found that the strains grown on PSA emit a wider spectrum and larger amount of VOCs compared with those grown on wheat kernels. F. langsethiae strain is the most active VOC producer on both substrates. The use of SPME and GC/MS also offers the potential for differentiation of fungal species and strains.
authors.affiliation: Research Institute of Hygiene, Occupational Pathology and Human Ecology Federal State Unitary Enterprise, Federal Medical Biological Agency, Build. 93, Kapitolovo Station, Kuz'molovsky g/p, Vsevolozhsky District, Leningrad Region, RU-188663.
authors.forename: Elena I
authors.initials: EI
authors.lastname: Savelieva
authors.affiliation: Research Institute of Hygiene, Occupational Pathology and Human Ecology Federal State Unitary Enterprise, Federal Medical Biological Agency, Build. 93, Kapitolovo Station, Kuz'molovsky g/p, Vsevolozhsky District, Leningrad Region, RU-188663.
authors.forename: Liudmila K
authors.initials: LK
authors.lastname: Gustyleva
authors.affiliation: Research Institute of Hygiene, Occupational Pathology and Human Ecology Federal State Unitary Enterprise, Federal Medical Biological Agency, Build. 93, Kapitolovo Station, Kuz'molovsky g/p, Vsevolozhsky District, Leningrad Region, RU-188663.
authors.forename: Elizaveta D
authors.initials: ED
authors.lastname: Kessenikh
authors.affiliation: Research Institute of Hygiene, Occupational Pathology and Human Ecology Federal State Unitary Enterprise, Federal Medical Biological Agency, Build. 93, Kapitolovo Station, Kuz'molovsky g/p, Vsevolozhsky District, Leningrad Region, RU-188663. esavelieva59@mail.ru.
authors.forename: Natalya S
authors.initials: NS
authors.lastname: Khlebnikova
authors.affiliation: Leffingwell & Associates, 4699 Arbor Hill Rd., Canton, GA, 30115, USA. leffingwell@mindspring.com.
authors.forename: John
authors.initials: J
authors.lastname: Leffingwell
authors.affiliation: All-Russian Institute of Plant Protection (VIZR) Podbelskogo shosse, 3 St.-Petersburg, Pushkin, RU-196608.
authors.forename: Olga P
authors.initials: OP
authors.lastname: Gavrilova
authors.affiliation: All-Russian Institute of Plant Protection (VIZR) Podbelskogo shosse, 3 St.-Petersburg, Pushkin, RU-196608.
authors.forename: Tatiana Yu
authors.initials: TY
authors.lastname: Gagkaeva
mesh_terms: D000362:Agar / Q000378:metabolism; D005670:Fusarium / Q000254:growth & development* / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D005740:Gases / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D012639:Seeds / Q000378:metabolism; D011198:Solanum tuberosum / Q000737:chemistry; D052617:Solid Phase Microextraction; D013395:Sucrose / Q000378:metabolism; D014908:Triticum; D055549:Volatile Organic Compounds / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D014835:Volatilization
chemical_list: D005740:Gases; D055549:Volatile Organic Compounds; D013395:Sucrose; D000362:Agar
keywords: Fusarium fungi; Gas chromatography/mass spectrometry; Headspace solid-phase microextraction; Retention indices; Volatile organic compounds