title: Mice deficient for striatal Vesicular Acetylcholine Transporter (VAChT) display impaired short-term but normal long-term object recognition memory.
abstract: Substantial evidence implicates Acetylcholine (ACh) in the acquisition of object memories. While most research has focused on the role of the cholinergic basal forebrain and its cortical targets, there are additional cholinergic networks that may contribute to object recognition. The striatum contains an independent cholinergic network comprised of interneurons. In the current study, we investigated the role of this cholinergic signalling in object recognition using mice deficient for Vesicular Acetylcholine Transporter (VAChT) within interneurons of the striatum. We tested whether these striatal VAChT(D2-Cre-flox/flox) mice would display normal short-term (5 or 15min retention delay) and long-term (3h retention delay) object recognition memory. In a home cage object recognition task, male and female VAChT(D2-Cre-flox/flox) mice were impaired selectively with a 15min retention delay. When tested on an object location task, VAChT(D2-Cre-flox/flox) mice displayed intact spatial memory. Finally, when object recognition was tested in a Y-shaped apparatus, designed to minimize the influence of spatial and contextual cues, only females displayed impaired recognition with a 5min retention delay, but when males were challenged with a 15min retention delay, they were also impaired; neither males nor females were impaired with the 3h delay. The pattern of results suggests that striatal cholinergic transmission plays a role in the short-term memory for object features, but not spatial location.
authors.affiliation: Department of Psychology, University of Guelph, Guelph, Ontario, Canada.
authors.forename: Daniel
authors.initials: D
authors.lastname: Palmer
authors.affiliation: Department of Psychology, University of Guelph, Guelph, Ontario, Canada.
authors.forename: Samantha
authors.initials: S
authors.lastname: Creighton
authors.affiliation: Robarts Research Institute, Schulich School of Medicine & Dentistry, University of Western Ontario, London, Ontario, Canada; Department of Physiology & Pharmacology, Schulich School of Medicine & Dentistry, University of Western Ontario, London, Ontario, Canada; Department of Anatomy & Cell Biology, Schulich School of Medicine & Dentistry, University of Western Ontario, London, Ontario, Canada.
authors.forename: Vania F
authors.initials: VF
authors.lastname: Prado
authors.affiliation: Robarts Research Institute, Schulich School of Medicine & Dentistry, University of Western Ontario, London, Ontario, Canada; Department of Physiology & Pharmacology, Schulich School of Medicine & Dentistry, University of Western Ontario, London, Ontario, Canada; Department of Anatomy & Cell Biology, Schulich School of Medicine & Dentistry, University of Western Ontario, London, Ontario, Canada.
authors.forename: Marco A M
authors.initials: MAM
authors.lastname: Prado
authors.affiliation: Department of Psychology, University of Guelph, Guelph, Ontario, Canada.
authors.forename: Elena
authors.initials: E
authors.lastname: Choleris
authors.affiliation: Department of Psychology, University of Guelph, Guelph, Ontario, Canada. Electronic address: bwinters@uoguelph.ca.
authors.forename: Boyer D
authors.initials: BD
authors.lastname: Winters
mesh_terms: D000818:Animals; D003342:Corpus Striatum / Q000378:metabolism*; D005260:Female; D007395:Interneurons / Q000378:metabolism; D008297:Male; D018782:Maze Learning / Q000502:physiology; D057567:Memory, Long-Term / Q000502:physiology*; D008570:Memory, Short-Term / Q000502:physiology*; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D021641:Recognition, Psychology / Q000502:physiology*; D012727:Sex Characteristics; D065852:Spatial Memory / Q000502:physiology*; D013997:Time Factors; D050494:Vesicular Acetylcholine Transport Proteins / Q000172:deficiency* / Q000235:genetics
chemical_list: C493460:Slc18a3 protein, mouse; D050494:Vesicular Acetylcholine Transport Proteins
keywords: Acetylcholine; Memory; Object location; Object recognition; Striatum; VAChT