title: Preliminary Proteomic Analysis of A549 Cells Infected with Avian Influenza Virus H7N9 and Influenza A Virus H1N1.
abstract: A newly emerged H7N9 influenza virus poses high risk to human beings. However, the pathogenic mechanism of the virus remains unclear. The temporal response of primary human alveolar adenocarcinoma epithelial cells (A549) infected with H7N9 influenza virus and H1N1 influenza A virus (H1N1, pdm09) were evaluated using the proteomics approaches (2D-DIGE combined with MALDI-TOF-MS/MS) at 24, 48 and 72 hours post of the infection (hpi). There were 11, 12 and 33 proteins with significant different expressions (P<0.05) at 24, 48 and 72hpi, especially F-actin-capping protein subunit alpha-1 (CAPZA1), Ornithine aminotransferase (OAT), Poly(rC)-binding protein 1 (PCBP1), Eukaryotic translation initiation factor 5A-1 (EIF5A) and Platelet-activating factor acetylhydrolaseⅠb subunit beta (PAFAH1B2) were validated by western-blot analysis. The functional analysis revealed that the differential proteins in A549 cells involved in regulating cytopathic effect. Among them, the down-regulation of CAPZA1, OAT, PCBP1, EIF5A are related to the death of cells infected by H7N9 influenza virus. This is the first time show that the down-regulation of PAFAH1B2 is related to the later clinical symptoms of patients infected by H7N9 influenza virus. These findings may improve our understanding of pathogenic mechanism of H7N9 influenza virus in proteomics.
authors.affiliation: School of Public Health, Sun Yat-sen University, Guangzhou, China.
authors.forename: Xiaoman
authors.initials: X
authors.lastname: Ding
authors.affiliation: School of Public Health, Sun Yat-sen University, Guangzhou, China.
authors.forename: Jiahai
authors.initials: J
authors.lastname: Lu
authors.affiliation: Southern Medical University, Guangzhou, China.
authors.forename: Ruoxi
authors.initials: R
authors.lastname: Yu
authors.affiliation: Major Infectious Disease Control Key Laboratory, Key Reference Laboratory of Pathogen and Biosafety, Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Nanshan Center for Disease Control and Prevention, Shenzhen, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.affiliation: Major Infectious Disease Control Key Laboratory, Key Reference Laboratory of Pathogen and Biosafety, Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
authors.forename: Fangyuan
authors.initials: F
authors.lastname: Dong
authors.affiliation: Major Infectious Disease Control Key Laboratory, Key Reference Laboratory of Pathogen and Biosafety, Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Peng
authors.affiliation: Major Infectious Disease Control Key Laboratory, Key Reference Laboratory of Pathogen and Biosafety, Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Wu
authors.affiliation: Major Infectious Disease Control Key Laboratory, Key Reference Laboratory of Pathogen and Biosafety, Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.affiliation: Major Infectious Disease Control Key Laboratory, Key Reference Laboratory of Pathogen and Biosafety, Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
authors.forename: Yijie
authors.initials: Y
authors.lastname: Geng
authors.affiliation: Major Infectious Disease Control Key Laboratory, Key Reference Laboratory of Pathogen and Biosafety, Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
authors.forename: Renli
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Major Infectious Disease Control Key Laboratory, Key Reference Laboratory of Pathogen and Biosafety, Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
authors.forename: Hanwu
authors.initials: H
authors.lastname: Ma
authors.affiliation: Major Infectious Disease Control Key Laboratory, Key Reference Laboratory of Pathogen and Biosafety, Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
authors.forename: Jinquan
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Nanshan Center for Disease Control and Prevention, Shenzhen, China.
authors.forename: Muhua
authors.initials: M
authors.lastname: Yu
authors.affiliation: Major Infectious Disease Control Key Laboratory, Key Reference Laboratory of Pathogen and Biosafety, Shenzhen Center for Disease Control and Prevention, Shenzhen, China.
authors.forename: Shisong
authors.initials: S
authors.lastname: Fang
mesh_terms: D000072283:A549 Cells; D003588:Cytopathogenic Effect, Viral; D005786:Gene Expression Regulation; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000378:metabolism*; D064766:Influenza A Virus, H7N9 Subtype / Q000378:metabolism*; D007251:Influenza, Human / Q000378:metabolism*; D020543:Proteome / Q000096:biosynthesis*
chemical_list: D020543:Proteome