title: Assay of GTPγS Binding in Autoradiography.
abstract: Autoradiography of radiolabeled GTPγS ([(35)S]GTPγS) binding is a relevant method to study the function of G protein-coupled receptors (GPCRs), in tissue sections. Here, we describe the protocol for such a binding autoradiography, suitable to investigate the functionality of CB1 receptor in tissue slices from rodent brain.
authors.affiliation: Department of Biotechnology and Life Sciences, University of Insubria, Via Manara 7, 21052, Busto Arsizio (VA), Italy.
authors.forename: Marina
authors.initials: M
authors.lastname: Gabaglio
authors.affiliation: Department of Biotechnology and Life Sciences, University of Insubria, Via Manara 7, 21052, Busto Arsizio (VA), Italy.
authors.forename: Pamela
authors.initials: P
authors.lastname: Prini
authors.affiliation: Department of Biotechnology and Life Sciences, University of Insubria, Via Manara 7, 21052, Busto Arsizio (VA), Italy.
authors.forename: Erica
authors.initials: E
authors.lastname: Zamberletti
authors.affiliation: Department of Biotechnology and Life Sciences, University of Insubria, Via Manara 7, 21052, Busto Arsizio (VA), Italy.
authors.forename: Tiziana
authors.initials: T
authors.lastname: Rubino
authors.affiliation: Department of Biotechnology and Life Sciences, University of Insubria, Via Manara 7, 21052, Busto Arsizio (VA), Italy. daniela.parolaro@uninsubria.it.|Fondazione Zardi-Gori, via Cossa 1, Milano, Italy. daniela.parolaro@uninsubria.it.
authors.forename: Daniela
authors.initials: D
authors.lastname: Parolaro
mesh_terms: D000818:Animals; D001345:Autoradiography / Q000379:methods; D001921:Brain / Q000378:metabolism; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000378:metabolism*; D007553:Isotope Labeling; D051379:Mice; D051381:Rats; D043884:Receptor, Cannabinoid, CB1 / Q000819:agonists / Q000378:metabolism; D013462:Sulfur Radioisotopes
chemical_list: D043884:Receptor, Cannabinoid, CB1; D013462:Sulfur Radioisotopes; D016244:Guanosine 5'-O-(3-Thiotriphosphate)
keywords: Autoradiography; Binding; Brain section; CB1 receptor; G-proteins; [35S]GTPγS