title: Plasma microRNA panels to diagnose pancreatic cancer: Results from a multicenter study.
abstract: Biomarkers for the early diagnosis of pancreatic cancer (PC) are urgent needed. Plasma microRNAs (miRNAs) might be used as biomarkers for the diagnosis of cancer. We analyzed 361 plasma samples from 6 surgical centers in China and performed machine learning approach. We gain insight of the association between the aberrant plasma miRNA expression and pancreatic disease. 671 microRNAs were screened in the discovery phase and 33 microRNAs in the training phase and 13 microRNAs in the validation phase. After the discovery phase and training phase, 2 diagnostic panels were constructed comprising 3 microRNAs in panel I (miR-486-5p, miR-126-3p, miR-106b-3p) and 6 microRNAs in panel II (miR-486-5p, miR-126-3p, miR-106b-3p, miR-938, miR-26b-3p, miR-1285). Panel I and panel II had high accuracy for distinguishing pancreatic cancer from chronic pancreatitis (CP) with area under the curve (AUC) values of 0.891 (Standard Error (SE): 0.097) and 0.889 (SE: 0.097) respectively, in the validation phase. Additionally, we demonstrated that the diagnostic value of the panels in discriminating PC from CP were comparable to that of carbohydrate antigen 19-9 (CA 19-9) 0.775 (SE: 0.053) (P = 0.1 for both). This study identified 2 diagnostic panels based on microRNA expression in plasma with the potential to distinguish PC from CP. These patterns might be developed as biomarkers for pancreatic cancer.
authors.affiliation: Department of General Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Cao
authors.affiliation: MOE Key Laboratory of Bioinformatics, Bioinformatics Division and Center for Synthetic and Systems Biology, TNLIST/Department of Automation, Tsinghua University, Beijing, 100084, China.
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of General Surgery, Qilu Hospital, Shandong University, Jinan, 250012, China.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of General Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, China.
authors.forename: Lei
authors.initials: L
authors.lastname: You
authors.affiliation: Department of General Surgery, Pancreatic Disease Institute, Wuhan Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, 430022, China.
authors.forename: Chunyou
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Pancreatic Surgery, Zhong Shan Hospital, Fudan University, Shanghai, 200032, China.
authors.forename: Wenhui
authors.initials: W
authors.lastname: Lou
authors.affiliation: Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Harbin Medical University, Harbin, 150001, China.
authors.forename: Bei
authors.initials: B
authors.lastname: Sun
authors.affiliation: Department of General Surgery, The First Affiliated Hospital, Nanjing Medical University, Nanjing, 210029, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Miao
authors.affiliation: Department of Hepatopancreatobiliary Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China.
authors.forename: Xubao
authors.initials: X
authors.lastname: Liu
authors.affiliation: MOE Key Laboratory of Bioinformatics, Bioinformatics Division and Center for Synthetic and Systems Biology, TNLIST/Department of Automation, Tsinghua University, Beijing, 100084, China.
authors.forename: Xiaowo
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, China.
authors.forename: Taiping
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, China.
authors.forename: Yupei
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics; D001774:Blood Chemical Analysis / Q000379:methods; D016022:Case-Control Studies; D002681:China; D055088:Early Detection of Cancer / Q000379:methods*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D056726:Genetic Association Studies / Q000379:methods; D057166:High-Throughput Screening Assays; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D010190:Pancreatic Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D050500:Pancreatitis, Chronic / Q000097:blood; D059467:Transcriptome
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs
keywords: diagnosis; microRNA panels; multicenter study; pancreatic cancer