title: Engineered Swine Models of Cancer.
abstract: Over the past decade, the technology to engineer genetically modified swine has seen many advancements, and because their physiology is remarkably similar to that of humans, swine models of cancer may be extremely valuable for preclinical safety studies as well as toxicity testing of pharmaceuticals prior to the start of human clinical trials. Hence, the benefits of using swine as a large animal model in cancer research and the potential applications and future opportunities of utilizing pigs in cancer modeling are immense. In this review, we discuss how pigs have been and can be used as a biomedical models for cancer research, with an emphasis on current technologies. We have focused on applications of precision genetics that can provide models that mimic human cancer predisposition syndromes. In particular, we describe the advantages of targeted gene-editing using custom endonucleases, specifically TALENs and CRISPRs, and transposon systems, to make novel pig models of cancer with broad preclinical applications.
authors.affiliation: Recombinetics St. Paul, MN, USA.
authors.forename: Adrienne L
authors.initials: AL
authors.lastname: Watson
authors.affiliation: Recombinetics St. Paul, MN, USA.
authors.forename: Daniel F
authors.initials: DF
authors.lastname: Carlson
authors.affiliation: RecombineticsSt. Paul, MN, USA; Masonic Cancer Center, University of MinnesotaMinneapolis, MN, USA; Genetics, Cell Biology and Development, University of MinnesotaMinneapolis, MN, USA; Pediatrics, University of MinnesotaMinneapolis, MN, USA.
authors.forename: David A
authors.initials: DA
authors.lastname: Largaespada
authors.affiliation: RecombineticsSt. Paul, MN, USA; Genetics, Cell Biology and Development, University of MinnesotaMinneapolis, MN, USA; Center for Genome Engineering, University of MinnesotaMinneapolis, MN, USA.
authors.forename: Perry B
authors.initials: PB
authors.lastname: Hackett
authors.affiliation: Recombinetics St. Paul, MN, USA.
authors.forename: Scott C
authors.initials: SC
authors.lastname: Fahrenkrug
keywords: cancer genetics; genetically engineered swine; genome engineering; preclinical cancer models; swine models