title: The relationship between adipocyte size and the transcript levels of SNAP23, BSCL2 and COPA genes in pigs.
abstract: Breed-specific differences in fat tissue accumulation in the pig provide an opportunity to study the genetic background of this process. In the present study three pig breeds, differing in fatness, were analyzed in terms of the size of adipocytes derived from three tissues (subcutaneous, visceral and longissimus dorsi muscle) in relation to transcript levels of genes (SNAP23, BSCL2 and COPA), which encode proteins involved in lipid droplet formation. The analysis of adipocyte size revealed significant effects of breed and tissue and confirmed earlier reports that an elevated backfat thickness in some pig breeds is correlated with a larger adipocyte size. Variability in the transcript abundance of the studied genes among breeds and tissues was observed. We found a positive correlation between the abundance of the SNAP23 transcript and adipocyte diameter. The obtained results indicate that SNAP23 may be considered as an interesting candidate gene involved in adipose tissue growth in the pig.
authors.affiliation: Department of Genetics and Animal Breeding, Poznan University of Life Sciences, 60-637 Poznan, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Kociucka
authors.affiliation: Department of Histology and Embryology, Institute of Zoology, Poznan University of Life Sciences, 60-625 Poznan, Poland.
authors.forename: Hanna
authors.initials: H
authors.lastname: Jackowiak
authors.affiliation: Pig Hybridization Centre, National Research Institute of Animal Production, 64-122 Pawlowice, Poland.
authors.forename: Marian
authors.initials: M
authors.lastname: Kamyczek
authors.affiliation: Department of Genetics and Animal Breeding, Poznan University of Life Sciences, 60-637 Poznan, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Szydlowski
authors.affiliation: Department of Genetics and Animal Breeding, Poznan University of Life Sciences, 60-637 Poznan, Poland. Electronic address: izabel@up.poznan.pl.
authors.forename: Izabela
authors.initials: I
authors.lastname: Szczerbal
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D001947:Breeding; D048429:Cell Size; D020756:Coatomer Protein / Q000235:genetics* / Q000378:metabolism; D044388:GTP-Binding Protein gamma Subunits / Q000235:genetics* / Q000378:metabolism; D060005:Genotyping Techniques; D016014:Linear Models; D018482:Muscle, Skeletal / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D050766:Qb-SNARE Proteins / Q000235:genetics* / Q000378:metabolism; D050767:Qc-SNARE Proteins / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013552:Swine / Q000235:genetics*; D059467:Transcriptome
chemical_list: D020756:Coatomer Protein; D044388:GTP-Binding Protein gamma Subunits; D050766:Qb-SNARE Proteins; D050767:Qc-SNARE Proteins
keywords: Adipose tissue; Cell size; Fatness; Gene expression; Lipid droplets; Pig