title: De novo transcriptome assembly of Sorghum bicolor variety Taejin.
abstract: Sorghum (Sorghum bicolor), also known as great millet, is one of the most popular cultivated grass species in the world. Sorghum is frequently consumed as food for humans and animals as well as used for ethanol production. In this study, we conducted de novo transcriptome assembly for sorghum variety Taejin by next-generation sequencing, obtaining 8.748 GB of raw data. The raw data in this study can be available in NCBI SRA database with accession number of SRX1715644. Using the Trinity program, we identified 222,161 transcripts from sorghum variety Taejin. We further predicted coding regions within the assembled transcripts by the TransDecoder program, resulting in a total of 148,531 proteins. We carried out BLASTP against the Swiss-Prot protein sequence database to annotate the functions of the identified proteins. To our knowledge, this is the first transcriptome data for a sorghum variety derived from Korea, and it can be usefully applied to the generation of genetic markers.
authors.affiliation: Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Yeonhwa
authors.initials: Y
authors.lastname: Jo
authors.affiliation: College of Crop Protection and Agronomy, Qingdao Agricultural University, Qingdao, Shandong 266109, China.
authors.forename: Sen
authors.initials: S
authors.lastname: Lian
authors.affiliation: The Taejin Genome Institute, Gadam-gil 61, Hoengseong, 25239, Republic of Korea.
authors.forename: Jin Kyong
authors.initials: JK
authors.lastname: Cho
authors.affiliation: Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Hoseong
authors.initials: H
authors.lastname: Choi
authors.affiliation: Crop Foundation Division, National Institute of Crop Science, RDA, Wanju, 55365, Republic of Korea.
authors.forename: Sang-Min
authors.initials: SM
authors.lastname: Kim
authors.affiliation: Crop Foundation Division, National Institute of Crop Science, RDA, Wanju, 55365, Republic of Korea.
authors.forename: Sun-Lim
authors.initials: SL
authors.lastname: Kim
authors.affiliation: Crop Foundation Division, National Institute of Crop Science, RDA, Wanju, 55365, Republic of Korea.
authors.forename: Bong Choon
authors.initials: BC
authors.lastname: Lee
authors.affiliation: Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea; The Taejin Genome Institute, Gadam-gil 61, Hoengseong, 25239, Republic of Korea.
authors.forename: Won Kyong
authors.initials: WK
authors.lastname: Cho
keywords: RNA-Seq; Sorghum bicolor; Transcriptome; Variety