title: An Hydroalcoholic Chamomile Extract Modulates Inflammatory and Immune Response in HT29 Cells and Isolated Rat Colon.
abstract: Inflammatory bowel diseases (IBDs) are chronic disorders characterized by disruption and ulceration of the colonic mucosa or of any part of the digestive tract (Crohn's disease). Antioxidant/anti-inflammatory herbal extract supplementation could represent an innovative approach to contrast IBDs. Clinical trials demonstrated the efficacy of natural formulas, containing chamomile, in patients with gastrointestinal disorders. This is consistent, albeit in part, with the antioxidant and anti-inflammatory properties of chamomile. The aim of the present study was to explore the possible protective role of a chamomile extract, on human colorectal adenocarcinoma HT29 cell, and rat colon specimens treated with lipopolysaccharide (LPS) to induce an inflammatory stimulus, a well established model of acute ulcerative colitis. In this context, the activities of different biomarkers of inflammation and lipid peroxidation such as ROS, myeloperoxidase (MPO), serotonin (5-HT), prostaglandin (PG)E2 , 8-iso-prostaglandin (8-iso-PG)F2α , NF-kB, tumor necrosis factor (TNF)α and interleukin (IL)-6 were assessed. We found that chamomile extract was as effective as sulfasalazine (2 mg/ml) in reducing the production of MPO, 5-HT, IL-6, NF-kB, TNFα, PGE2 and 8-iso-PGF2α , after inflammatory stimulus. The observed modulatory effects support a rationale use of chamomile supplementation as a promising pharmacological tool for the prevention and management of ulcerative colitis in humans. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Dipartimento di Farmacia, Università 'G. D'Annunzio' Chieti-Pescara, Via dei Vestini 31, 66100, Chieti, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Menghini
authors.affiliation: Dipartimento di Farmacia, Università 'G. D'Annunzio' Chieti-Pescara, Via dei Vestini 31, 66100, Chieti, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Ferrante
authors.affiliation: Dipartimento di Farmacia, Università 'G. D'Annunzio' Chieti-Pescara, Via dei Vestini 31, 66100, Chieti, Italy.
authors.forename: Lidia
authors.initials: L
authors.lastname: Leporini
authors.affiliation: Dipartimento di Farmacia, Università 'G. D'Annunzio' Chieti-Pescara, Via dei Vestini 31, 66100, Chieti, Italy.
authors.forename: Lucia
authors.initials: L
authors.lastname: Recinella
authors.affiliation: Dipartimento di Farmacia, Università 'G. D'Annunzio' Chieti-Pescara, Via dei Vestini 31, 66100, Chieti, Italy.
authors.forename: Annalisa
authors.initials: A
authors.lastname: Chiavaroli
authors.affiliation: Dipartimento di Farmacia, Università 'G. D'Annunzio' Chieti-Pescara, Via dei Vestini 31, 66100, Chieti, Italy.
authors.forename: Sheila
authors.initials: S
authors.lastname: Leone
authors.affiliation: Dipartimento di Chimica e Farmacia, Università di Sassari, Via Muroni 23, 07100, Sassari, Italy.
authors.forename: Giorgio
authors.initials: G
authors.lastname: Pintore
authors.affiliation: Dipartimento di Farmacia, Università 'G. D'Annunzio' Chieti-Pescara, Via dei Vestini 31, 66100, Chieti, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Vacca
authors.affiliation: Dipartimento di Farmacia, Università 'G. D'Annunzio' Chieti-Pescara, Via dei Vestini 31, 66100, Chieti, Italy.
authors.forename: Giustino
authors.initials: G
authors.lastname: Orlando
authors.affiliation: Dipartimento di Farmacia, Università 'G. D'Annunzio' Chieti-Pescara, Via dei Vestini 31, 66100, Chieti, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Brunetti
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D020843:Chamomile / Q000737:chemistry*; D003106:Colon / Q000473:pathology*; D003424:Crohn Disease / Q000188:drug therapy*; D019073:HT29 Cells; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000188:drug therapy*; D008297:Male; D018384:Oxidative Stress; D010936:Plant Extracts / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000893:Anti-Inflammatory Agents; D010936:Plant Extracts
keywords: chamomile; colon; immunomodulation; inflammation; oxidative Stress