title: Haem-assisted dityrosine-cross-linking of fibrinogen under non-thermal plasma exposure: one important mechanism of facilitated blood coagulation.
abstract: Although blood coagulation facilitated by non-thermal plasma has been reported several years ago, the insight to the involved mechanisms is still rather limited. In this work, we report our discovery of a new mechanism for the haem-promoted blood-coagulation caused by non-thermal plasma treatment. The reason for the haem role is due to that its oxidized form, namely, hematin, can promote the dityrosine cross-linking of fibrinogen, the most important coagulation protein, to form a membrane-like layer on the surface of the treated blood with plasma exposure. Both haem and non-thermal-plasma generated hydrogen peroxide are requisite for the cross-linking process. We confirmed that fibrinogen can coordinate with the haem iron to form a protein-haem complex which shows pseudo-peroxidase activity, and in the presence of hydrogen peroxide, the complex can induce the dityrosine formation between fibrinogen molecules, leading to the fibrin network necessary for the blood coagulation. Understanding of such an underlying mechanism can be useful to guide more efficient application of non-thermal plasma in the management of hemostasis, thrombosis and etc.
authors.affiliation: Key Laboratory of Ion Beam Bioengineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Ke
authors.affiliation: Key Laboratory of Ion Beam Bioengineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China.|National Synchrotron Radiation Laboratory, University of Science &Technology of China, Hefei 230026, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Huang
mesh_terms: D001773:Blood Cells / Q000737:chemistry / Q000166:cytology; D001777:Blood Coagulation; D056831:Coordination Complexes / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D005340:Fibrinogen / Q000737:chemistry*; D006418:Heme / Q000737:chemistry*; D006427:Hemin / Q000737:chemistry; D006801:Humans; D007501:Iron / Q000737:chemistry; D058626:Plasma Gases / Q000494:pharmacology*; D061251:Primary Cell Culture; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D056831:Coordination Complexes; D003432:Cross-Linking Reagents; D058626:Plasma Gases; D014443:Tyrosine; D006418:Heme; D006427:Hemin; D005340:Fibrinogen; C007543:dityrosine; D007501:Iron