title: Mammalian Cell Tissue Culture Techniques.
abstract: Cultured tissues and cells are used extensively in physiological and pharmacological studies. In vitro cultures provide a means of examining cells and tissues without the complex interactions that would be present if the whole organism were studied. A number of special skills are required in order to preserve the structure, function, behavior, and biology of cells in culture. This unit describes the basic skills required to maintain and preserve cell cultures: maintaining aseptic technique, preparing media with the appropriate characteristics, passaging, freezing and storage, recovering frozen stocks, and counting viable cells. © 2016 by John Wiley & Sons, Inc.
authors.affiliation: Hayward Genetics Program and Department of Pediatrics, Tulane University School of Medicine, New Orleans, Louisiana.
authors.forename: Katy
authors.initials: K
authors.lastname: Phelan
authors.affiliation: Genetic Diagnostic Laboratory, Children's Hospital at Erlanger, Chattanooga, Tennessee.
authors.forename: Kristin M
authors.initials: KM
authors.lastname: May
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D002478:Cells, Cultured; D003470:Culture Media; D004866:Equipment Contamination / Q000517:prevention & control; D006801:Humans; D008322:Mammals; D009174:Mycoplasma / Q000378:metabolism; D046509:Tissue Culture Techniques / Q000379:methods*
chemical_list: D003470:Culture Media
keywords: aseptic technique; freezing cells; medium; passaging cells; tissue culture