title: Molecular epidemiology and phylogenetic analysis of HA gene of influenza A(H1N1)pdm09 strain during 2010-2014 in Dalian, North China.
abstract: The objective of the present study was to evaluate the epidemiology of influenza A(H1N1)pdm09 and its hemagglutinin (HA) molecular and phylogenetic analysis during 2010-2014 in Dalian, North China. A total of 3717 influenza-like illness (ILI) cases were tested by real-time PCR and 493 were found to be positive. Out of these 493 cases, 121 were subtype influenza A(H1N1)pdm09, of which 14 cases were reported in 2010-2011, 29 in 2012-2013, and 78 in 2013-2014. HA coding regions of 45 isolates were compared to that of the vaccine strain A/California/7/09(H1N1), and a number of variations were detected. P83S, S185T, S203T, R223Q, and I321V mutations were observed in all of the Dalian isolates. Furthermore, a high proportion >71 % of the strains possessed the variation D97N and K283E. Phylogenetic analysis confirmed the close match of the majority of circulating strains with the vaccine strains. However, it also reveals a trend of strains to accumulate amino acid variations and form new phylogenetic groups.
authors.affiliation: Dalian Center for Disease Control and Prevention, Dalian, 116021, China. 18940956816@163.com.
authors.forename: Yan
authors.identifier: http://orcid.org/0000-0002-5203-7230
authors.initials: Y
authors.lastname: Han
authors.affiliation: Dalian Center for Disease Control and Prevention, Dalian, 116021, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Sun
authors.affiliation: Dalian Center for Disease Control and Prevention, Dalian, 116021, China.
authors.forename: Qiu-Yue
authors.initials: QY
authors.lastname: Lv
authors.affiliation: Dalian Center for Disease Control and Prevention, Dalian, 116021, China.
authors.forename: Dan-Hong
authors.initials: DH
authors.lastname: Liu
authors.affiliation: Dalian Center for Disease Control and Prevention, Dalian, 116021, China.
authors.forename: Da-Peng
authors.initials: DP
authors.lastname: Liu
mesh_terms: D000596:Amino Acids / Q000235:genetics; D002681:China / Q000453:epidemiology; D019143:Evolution, Molecular; D005838:Genotype; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics*; D006388:Hemagglutinins / Q000235:genetics; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics*; D007251:Influenza, Human / Q000453:epidemiology* / Q000821:virology*; D017720:Molecular Epidemiology / Q000379:methods; D009154:Mutation / Q000235:genetics; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D000596:Amino Acids; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006388:Hemagglutinins; D012367:RNA, Viral
keywords: Influenza A(H1N1)pdm09; Phylogenetic analysis