title: Chikungunya Virus Infection of Aedes Mosquitoes.
abstract: In vivo infection of mosquitoes is an important method to study and characterize arthropod-borne viruses. Chikungunya virus (CHIKV) is a mosquito-borne alphavirus that is transmitted primarily by Aedes mosquitoes. In this chapter, we describe a protocol for infection of CHIKV in two species of Aedes mosquitoes, Aedes aegypti and Aedes albopictus, together with the isolation of CHIKV in different parts of the infected mosquito such as midgut, legs, wings, salivary gland, head, and saliva. This allows the study of viral infection, replication and dissemination within the mosquito vector.
authors.affiliation: Department of Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, 50603, Malaysia.
authors.forename: Hui Vern
authors.initials: HV
authors.lastname: Wong
authors.affiliation: Department of Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, 50603, Malaysia.
authors.forename: Yoke Fun
authors.initials: YF
authors.lastname: Chan
authors.affiliation: Department of Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, 50603, Malaysia.
authors.forename: I-Ching
authors.initials: IC
authors.lastname: Sam
authors.affiliation: Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, 50603, Malaysia.
authors.forename: Wan Yusof Wan
authors.initials: WY
authors.lastname: Sulaiman
authors.affiliation: Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, 50603, Malaysia. indrav@um.edu.my.
authors.forename: Indra
authors.initials: I
authors.lastname: Vythilingam
mesh_terms: D000330:Aedes / Q000821:virology*; D018354:Alphavirus Infections / Q000821:virology*; D000818:Animals; D002646:Chikungunya virus / Q000302:isolation & purification / Q000472:pathogenicity* / Q000502:physiology; D005260:Female; D006257:Head / Q000821:virology; D006801:Humans; D007303:Insect Vectors / Q000821:virology; D007422:Intestines / Q000821:virology; D035002:Lower Extremity / Q000821:virology; D012463:Saliva / Q000821:virology; D012469:Salivary Glands / Q000821:virology; D014779:Virus Replication; D014921:Wings, Animal / Q000821:virology
keywords: Aedes aegypti; Aedes albopictus; Alphavirus; Arbovirus; Blood meal; Chikungunya virus; Oral infection