title: Differentially Expressed miRNAs in Tumor, Adjacent, and Normal Tissues of Lung Adenocarcinoma.
abstract: Lung cancer is the leading cause of cancer deaths. Non-small-cell lung cancer (NSCLC) is the major type of lung cancer. The aim of this study was to characterize the expression profiles of miRNAs in adenocarcinoma (AC), one major subtype of NSCLC. In this study, the miRNAs were detected in normal, adjacent, and tumor tissues by next-generation sequencing. Then the expression levels of differential miRNAs were quantified by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). In the results, 259, 401, and 389 miRNAs were detected in tumor, adjacent, and normal tissues of pooled AC samples, respectively. In addition, for the first time we have found that miR-21-5p and miR-196a-5p were gradually upregulated from normal to adjacent to tumor tissues; miR-218-5p was gradually downregulated with 2-fold or greater change in AC tissues. These 3 miRNAs were validated by qRT-PCR. Lastly, we predicted target genes of these 3 miRNAs and enriched the potential functions and regulatory pathways. The aberrant miR-21-5p, miR-196a-5p, and miR-218-5p may become biomarkers for diagnosis and prognosis of lung adenocarcinoma. This research may be useful for lung adenocarcinoma diagnosis and the study of pathology in lung cancer.
authors.affiliation: Research Center for Learning Science, Southeast University, Nanjing 210096, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Tian
authors.affiliation: Research Center for Learning Science, Southeast University, Nanjing 210096, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Li
authors.affiliation: Research Center for Learning Science, Southeast University, Nanjing 210096, China.
authors.forename: Zhenzhu
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Research Center for Learning Science, Southeast University, Nanjing 210096, China.
authors.forename: Yanting
authors.initials: Y
authors.lastname: Shen
authors.affiliation: State Key Lab of Bioelectronics, Southeast University, Nanjing 210096, China.
authors.forename: Jiafeng
authors.initials: J
authors.lastname: Lu
authors.affiliation: Research Center for Learning Science, Southeast University, Nanjing 210096, China.
authors.forename: Xueying
authors.initials: X
authors.lastname: Xie
authors.affiliation: Research Center for Learning Science, Southeast University, Nanjing 210096, China; State Key Lab of Bioelectronics, Southeast University, Nanjing 210096, China.
authors.forename: Qinyu
authors.initials: Q
authors.lastname: Ge
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis* / Q000235:genetics*; D014408:Biomarkers, Tumor / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D011379:Prognosis; D015203:Reproducibility of Results; D018570:Risk Assessment; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; D005819:Genetic Markers; D035683:MicroRNAs