title: Characterisation of a novel enterobacteria phage, CAjan, isolated from rat faeces.
abstract: In this study, we describe the isolation and characterisation of the novel enterobacteria phage CAjan. This phage belongs to the order Caudovirales and the family Siphoviridae. The phage possesses a linear, double-stranded DNA genome consisting of 59,670 bp with a G+C content of 44.7 % and 91 predicted open reading frames (ORFs). Putative functions were assigned to 39 of the ORFs (37.4 %). The phage structural genes were furthermore functionally characterised by LC MS/MS. CAjan, together with Escherichia phage Seurat and Escherichia phage slur01, represent a novel and genetically distinct clade of Siphoviridae phages that could be considered to constitute a new phage genus. Despite limited sequence similarity, the phages in this group share a number of other common features, including genome structure and the presence of queuosine biosynthesis genes.
authors.affiliation: Department of Environmental Science, Aarhus University, Frederiksborgvej, 399, 4000, Roskilde, Denmark.
authors.forename: Alexander B
authors.initials: AB
authors.lastname: Carstens
authors.affiliation: Department of Environmental Science, Aarhus University, Frederiksborgvej, 399, 4000, Roskilde, Denmark. wk@envs.au.dk.
authors.forename: Witold
authors.initials: W
authors.lastname: Kot
authors.affiliation: Department of Food Science, Faculty of Science, University of Copenhagen, Rolighedsvej 26, 1958, Frederiksberg C, Denmark.
authors.forename: Rene
authors.initials: R
authors.lastname: Lametsch
authors.affiliation: Department of Microbiology and Biotechnology, Max Rubner-Institut, Hermann-Weigmann-Straße 1, 24103, Kiel, Germany.
authors.forename: Horst
authors.initials: H
authors.lastname: Neve
authors.affiliation: Department of Environmental Science, Aarhus University, Frederiksborgvej, 399, 4000, Roskilde, Denmark.
authors.forename: Lars H
authors.initials: LH
authors.lastname: Hansen
mesh_terms: D000818:Animals; D001435:Bacteriophages / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001482:Base Composition; D004755:Enterobacteriaceae / Q000821:virology; D005243:Feces / Q000821:virology*; D016679:Genome, Viral; D016366:Open Reading Frames; D010802:Phylogeny; D051381:Rats; D017906:Siphoviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins