title: A high-resolution method to assess cell multinucleation with cytoplasm-localized fluorescent probes.
abstract: Cell multinucleation is closely related to chromosomal instability. We report a simple, convenient method to assess cell multinucleation with cytoplasm-localized fluorescent probes (CLFP) which is superior to conventional nuclear staining methods. The CLFP method provides high-resolution images that enable the accurate calculation of the number of nuclear fragments.
authors.affiliation: State Key Laboratory of Bioactive Substances and Function of Natural Medicine, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, 100050, China. hcui@imm.ac.cn yindali@imm.ac.cn and Beijing Key Laboratory of Active Substances Discovery and Drugability Evaluation, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, 100050, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wen
authors.affiliation: College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, 250355, China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Cui
authors.affiliation: College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, 250355, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Meng
authors.affiliation: State Key Laboratory of Bioactive Substances and Function of Natural Medicine, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, 100050, China. hcui@imm.ac.cn yindali@imm.ac.cn.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Lai
authors.affiliation: Beijing Key Laboratory of Active Substances Discovery and Drugability Evaluation, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, 100050, China.
authors.forename: Shufang
authors.initials: S
authors.lastname: Wang
authors.affiliation: Beijing Key Laboratory of Active Substances Discovery and Drugability Evaluation, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, 100050, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Bioactive Substances and Function of Natural Medicine, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, 100050, China. hcui@imm.ac.cn yindali@imm.ac.cn.
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Bioactive Substances and Function of Natural Medicine, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, 100050, China. hcui@imm.ac.cn yindali@imm.ac.cn and Beijing Key Laboratory of Active Substances Discovery and Drugability Evaluation, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, 100050, China.
authors.forename: Huaqing
authors.initials: H
authors.lastname: Cui
authors.affiliation: State Key Laboratory of Bioactive Substances and Function of Natural Medicine, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, 100050, China. hcui@imm.ac.cn yindali@imm.ac.cn.
authors.forename: Dali
authors.initials: D
authors.lastname: Yin
mesh_terms: D001896:Boron Compounds; D045744:Cell Line, Tumor; D002467:Cell Nucleus; D003593:Cytoplasm / Q000737:chemistry*; D053938:DNA Fragmentation; D003619:Dansyl Compounds; D005456:Fluorescent Dyes; D006801:Humans; D013194:Staining and Labeling
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D003619:Dansyl Compounds; D005456:Fluorescent Dyes; C029038:dansyl chloride