title: Measuring the Expression of microRNAs Regulated by Androgens.
abstract: The discovery of microRNAs (miRNAs) provided yet another mechanism of gene expression regulation. miRNAs have recently been also implicated in many diseases, including prostate cancer (PC). As PC is a highly androgen-dependent disease, extensive effort has been invested to identify the miRNAs that are androgen regulated. However, relatively few of them have been shown to be directly androgen regulated in PC. In this chapter we introduce the commonly used techniques to study the androgen regulation of miRNAs. The most cost-effective tool to profile global miRNA expression is microarray-based hybridization, whereas real-time quantitative reverse transcription PCR (qRT-PCR) is commonly used for the study of individual miRNAs.
authors.affiliation: Prostate Cancer Research Center, Institute of Biosciences and Medical Technology-BioMediTech and Fimlab Laboratories, University of Tampere and Tampere University Hospital, Tampere, Finland.
authors.forename: Mauro
authors.initials: M
authors.lastname: Scaravilli
authors.affiliation: Prostate Cancer Research Center, Institute of Biosciences and Medical Technology-BioMediTech and Fimlab Laboratories, University of Tampere and Tampere University Hospital, Tampere, Finland.
authors.forename: Kati
authors.initials: K
authors.lastname: Kivinummi
authors.affiliation: Prostate Cancer Research Center, Institute of Biosciences and Medical Technology-BioMediTech and Fimlab Laboratories, University of Tampere and Tampere University Hospital, Tampere, Finland.
authors.forename: Tapio
authors.initials: T
authors.lastname: Visakorpi
authors.affiliation: Prostate Cancer Research Center, Institute of Biosciences and Medical Technology-BioMediTech and Fimlab Laboratories, University of Tampere and Tampere University Hospital, Tampere, Finland. leena.latonen@uta.fi.|Institute of Biosciences and Medical Technology, BioMediTech University of Tampere, Biokatu 6, 33520, Tampere, Finland. leena.latonen@uta.fi.
authors.forename: Leena
authors.initials: L
authors.lastname: Latonen
mesh_terms: D000728:Androgens / Q000494:pharmacology*; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011471:Prostatic Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D014407:Tumor Cells, Cultured
chemical_list: D000728:Androgens; D035683:MicroRNAs
keywords: Androgen receptor; Androgens; Gene expression; Microarray; Prostate cancer; microRNA; qRT-PCR