title: A new reassortment of influenza A (H7N9) virus causing human infection in Beijing, 2014.
abstract: A 73-year-old man was confirmed to have an influenza A (H7N9) virus infection, and the causative agent A/Beijing/02/2014(H7N9) virus was isolated. Genetic and phylogenetic analyses revealed that the virus belonged to a novel genotype, which probably emerged and further reassorted with other H9 or H7 viruses in poultry before transmitting to humans. This virus caused a severe infection with high levels of cytokines and neutralizing antibodies. Eventually, the patient was cured after serially combined treatments. Taken together, our findings indicated that this novel genotype of the human H7N9 virus did not evolve directly from the first Beijing isolate A/Beijing/01/2013(H7N9), suggesting that the H7N9 virus has not obtained the ability for human-to-human transmissibility and the virus only evolves in poultry and then infects human by direct contact. Hence, the major measures to prevent human H7N9 virus infection are still to control and standardize the live poultry trade. Early antiviral treatment with combination therapies, including mechanical ventilation, nutrition support and symptomatic treatment, are effective for H7N9 infection.
authors.affiliation: Shenzhen Key Laboratory of Pathogen and Immunity, Shenzhen Third People's Hospital, Shenzhen 518112, China.|CAS Key Laboratory of Pathogenic Microbiology and Immunology (CASPMI), Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.|Center for Influenza Research and Early-warning (CASCIRE), Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Yuhai
authors.initials: Y
authors.lastname: Bi
authors.affiliation: Intensive Care Unit, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China.
authors.forename: Jingyuan
authors.initials: J
authors.lastname: Liu
authors.affiliation: Intensive Care Unit, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China.
authors.forename: Haofeng
authors.initials: H
authors.lastname: Xiong
authors.affiliation: Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China.|Beijing Key Laboratory of Emerging Infectious Diseases, Beijing 100015, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology (CASPMI), Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.|Center for Influenza Research and Early-warning (CASCIRE), Chinese Academy of Sciences, Beijing 100101, China.|Network Information Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Di
authors.initials: D
authors.lastname: Liu
authors.affiliation: Shenzhen Key Laboratory of Pathogen and Immunity, Shenzhen Third People's Hospital, Shenzhen 518112, China.
authors.forename: Yingxia
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Shenzhen Key Laboratory of Pathogen and Immunity, Shenzhen Third People's Hospital, Shenzhen 518112, China.|CAS Key Laboratory of Pathogenic Microbiology and Immunology (CASPMI), Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.|Center for Influenza Research and Early-warning (CASCIRE), Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: George F
authors.initials: GF
authors.lastname: Gao
authors.affiliation: Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China.|Beijing Key Laboratory of Emerging Infectious Diseases, Beijing 100015, China.
authors.forename: Beibei
authors.initials: B
authors.lastname: Wang
mesh_terms: D000368:Aged; D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D002681:China; D004285:Dogs; D006801:Humans; D064766:Influenza A Virus, H7N9 Subtype / Q000276:immunology / Q000302:isolation & purification; D007251:Influenza, Human / Q000276:immunology* / Q000628:therapy; D061985:Madin Darby Canine Kidney Cells; D008297:Male
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral