title: Expression and Function of Histone Deacetylase 10 (HDAC10) in B Cell Malignancies.
abstract: Histone deacetylase 10 (HDAC10) belongs to the class IIb HDAC family and its biological role remains mostly unidentified. A decreased HDAC10 expression has been reported in patients with aggressive solid tumors (Osada et al. Int J Cancer 112: 26-32, 2004; Jin et al. Int J Clin Exp Pathol 7: 5872-5879, 2014), suggesting that loss of HDAC10 expression might confer a survival advantage to malignant cells. Consequently, results from our lab suggests that overexpression of HDAC10 in aggressive mantle cell lymphoma (MCL) and chronic lymphocytic leukemia (CLL) Z138c and MEC1 cells, respectively, resulted in a rapid induction of cell death in vitro with only 5 % of cells being alive at 48 h, cell cycle arrest, and up-regulation of co-stimulatory molecules. Here we present several standard methods to study the function of HDAC10 in B cell malignancies.
authors.affiliation: Department of Immunology, H. Lee Moffitt Cancer Center & Research Institute, SRB-2 Room 22344A2, 12902 Magnolia Drive, Tampa, FL, USA.|Department of Malignant Hematology, H. Lee Moffitt Cancer Center & Research Institute, Tampa, FL, USA.
authors.forename: John
authors.initials: J
authors.lastname: Powers
authors.affiliation: Department of Immunology, H. Lee Moffitt Cancer Center & Research Institute, SRB-2 Room 22344A2, 12902 Magnolia Drive, Tampa, FL, USA.
authors.forename: Maritza
authors.initials: M
authors.lastname: Lienlaf
authors.affiliation: Department of Immunology, H. Lee Moffitt Cancer Center & Research Institute, SRB-2 Room 22344A2, 12902 Magnolia Drive, Tampa, FL, USA.
authors.forename: Patricio
authors.initials: P
authors.lastname: Perez-Villarroel
authors.affiliation: Department of Immunology, H. Lee Moffitt Cancer Center & Research Institute, SRB-2 Room 22344A2, 12902 Magnolia Drive, Tampa, FL, USA.
authors.forename: Susan
authors.initials: S
authors.lastname: Deng
authors.affiliation: Department of Immunology, H. Lee Moffitt Cancer Center & Research Institute, SRB-2 Room 22344A2, 12902 Magnolia Drive, Tampa, FL, USA.
authors.forename: Tessa
authors.initials: T
authors.lastname: Knox
authors.affiliation: Department of Immunology, H. Lee Moffitt Cancer Center & Research Institute, SRB-2 Room 22344A2, 12902 Magnolia Drive, Tampa, FL, USA.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Villagra
authors.affiliation: Department of Immunology, H. Lee Moffitt Cancer Center & Research Institute, SRB-2 Room 22344A2, 12902 Magnolia Drive, Tampa, FL, USA. eva.sahakian@moffitt.org.|Department of Malignant Hematology, H. Lee Moffitt Cancer Center & Research Institute, Tampa, FL, USA. eva.sahakian@moffitt.org.
authors.forename: Eva
authors.initials: E
authors.lastname: Sahakian
mesh_terms: D000818:Animals; D015153:Blotting, Western; D049109:Cell Proliferation; D002470:Cell Survival; D015536:Down-Regulation; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D057166:High-Throughput Screening Assays; D006655:Histone Deacetylases / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics* / Q000378:metabolism*; D020522:Lymphoma, Mantle-Cell / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: C447828:HDAC10 protein, human; D006655:Histone Deacetylases
keywords: Adenoviral overexpression; Genotyping; High-throughput flow screening; Histone deacetylase 10; PCR; Western blotting; qRT-PCR