title: A Simple Spectrofluorimetric Method Based on Quenching of a Nickel(II)-Phthalocyanine Complex to Determine Iron (III).
abstract: A new nickel(II)-phthalocyanine complex (NiPc) was synthesized and used as a fluorescent ligand in determination of iron in real samples. The NiPc compound, when excited at 350 nm, decreases of emission with increases of the iron(III) concentration at 425 nm were used analytical response in a modified standard addition method. The method was validated by analyzing two certified reference materials (CRM-SA-C Sandy Soil C and Mixed Polish Herbs (INCT-MPH-2). Food and drug samples were digested in a closed microwave system using nitric acid and hydrogen peroxide. Therefore, all iron in the samples converted to iron(III) ion. These solutions were used directly in determination of iron(III) ion. No cleanup or enrichment of the solutions was required. The calibration graph was linear until 14.00 μg mL(-1). Detection limit and quantification limit were 1.29 μg mL(-1) and 3.88 μg mL(-1), respectively. The method provided accurate results for the majority of the food samples tested, including spanich, dill, mint, purslane, rocket, red lentils, dry beans and two iron medicinal tablets. Also, the high recovery (95.6 %) was obtained for a fortified stream water sample. The simple and cost-effective method is suitable for monitoring total iron concentration in foods and drug samples.
authors.affiliation: Department of Chemistry, Faculty of Sciences, Karadeniz Technical University, 61080, Trabzon, Turkey.
authors.forename: Yasemin
authors.initials: Y
authors.lastname: Çağlar
authors.affiliation: Department of Chemistry, Faculty of Sciences, Karadeniz Technical University, 61080, Trabzon, Turkey.
authors.forename: Ece Tuğba
authors.initials: ET
authors.lastname: Saka
authors.affiliation: Department of Chemistry, Faculty of Sciences, Karadeniz Technical University, 61080, Trabzon, Turkey.
authors.forename: Hakan
authors.initials: H
authors.lastname: Alp
authors.affiliation: Department of Chemistry, Faculty of Sciences, Karadeniz Technical University, 61080, Trabzon, Turkey.
authors.forename: Halit
authors.initials: H
authors.lastname: Kantekin
authors.affiliation: Department of Chemistry, Faculty of Sciences, Karadeniz Technical University, 61080, Trabzon, Turkey. uocak@ktu.edu.tr.
authors.forename: Ümmühan
authors.initials: Ü
authors.lastname: Ocak
authors.affiliation: Department of Chemistry, Faculty of Sciences, Karadeniz Technical University, 61080, Trabzon, Turkey.
authors.forename: Miraç
authors.initials: M
authors.lastname: Ocak
mesh_terms: D056831:Coordination Complexes / Q000737:chemistry*; D005504:Food Analysis; D006863:Hydrogen-Ion Concentration; D007211:Indoles / Q000737:chemistry*; D007501:Iron / Q000032:analysis* / Q000737:chemistry; D054833:Isoindoles; D009532:Nickel / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013997:Time Factors
chemical_list: D056831:Coordination Complexes; D007211:Indoles; D054833:Isoindoles; D009532:Nickel; D007501:Iron; C013647:phthalocyanine
keywords: Iron; Phthalocyanine; Quenching; Spectrofluorimetry; Standard addition method