title: A Hydrogel-Based Hybrid Theranostic Contact Lens for Fungal Keratitis.
abstract: Fungal keratitis, a severe ocular disease, is one of the leading causes of ocular morbidity and blindness, yet it is often neglected, especially in developing countries. Therapeutic efficacy of traditional treatment such as eye drops is very limited due to poor bioavailability, whereas intraocular injection might cause serious side effects. Herein, we designed and fabricated a hybrid hydrogel-based contact lens which comprises quaternized chitosan (HTCC), silver nanoparticles, and graphene oxide (GO) with a combination of antibacterial and antifungal functions. The hydrogel is cross-linked through electrostatic interactions between GO and HTCC, resulting in strong mechanical properties. Voriconazole (Vor), an antifungal drug, can be loaded onto GO which retains the drug and promotes its sustained release from the hydrogel-based contact lenses. The contact lenses also exhibited good antimicrobial functions in view of glycidyltrimethylammonium chloride and silver nanoparticles. The results from in vitro and in vivo experiments demonstrate that contact lenses loaded with Vor have excellent efficacy in antifungal activity in vitro and could significantly enhance the therapeutic effects on a fungus-infected mouse model. The results indicate that this hydrogel contact lenses-based drug delivery system might be a promising therapeutic approach for a rapid and effective treatment of fungal keratitis.
authors.affiliation: Department of Pharmaceutical Engineering, College of Materials and Energy, South China Agricultural University , Guangzhou 510642, China.
authors.forename: Jian-Fei
authors.initials: JF
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Centre, Sun Yat-Sen University , Guangzhou 510064, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhong
authors.affiliation: Department of Pharmaceutical Engineering, College of Materials and Energy, South China Agricultural University , Guangzhou 510642, China.
authors.forename: Guo-Pu
authors.initials: GP
authors.lastname: Chen
authors.affiliation: Department of Biomedical Engineering, University of Houston , Houston, Texas 77204, United States.
authors.forename: Zuan-Tao
authors.initials: ZT
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Centre, Sun Yat-Sen University , Guangzhou 510064, China.
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Department of Pharmaceutical Engineering, College of Materials and Energy, South China Agricultural University , Guangzhou 510642, China.
authors.forename: Yong-Lin
authors.initials: YL
authors.lastname: Liu
authors.affiliation: Department of Pharmaceutical Engineering, College of Materials and Energy, South China Agricultural University , Guangzhou 510642, China.
authors.forename: Piao-Yang
authors.initials: PY
authors.lastname: Cao
authors.affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Centre, Sun Yat-Sen University , Guangzhou 510064, China.
authors.forename: Bowen
authors.initials: B
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Centre, Sun Yat-Sen University , Guangzhou 510064, China.
authors.forename: Yantao
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Department of Biomedical Engineering, University of Houston , Houston, Texas 77204, United States.
authors.forename: Tianfu
authors.initials: T
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Centre, Sun Yat-Sen University , Guangzhou 510064, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Department of Pharmaceutical Engineering, College of Materials and Energy, South China Agricultural University , Guangzhou 510642, China.
authors.forename: Gang-Biao
authors.initials: GB
authors.lastname: Jiang
mesh_terms: D000818:Animals; D003261:Contact Lenses; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D020100:Hydrogels; D007634:Keratitis / Q000628:therapy*; D053768:Metal Nanoparticles; D051379:Mice; D012834:Silver; D000068936:Theranostic Nanomedicine
chemical_list: D020100:Hydrogels; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D012834:Silver
keywords: antifungal; fungal keratitis; graphene oxide; nanosilver; ophthalmology treatment