title: Upregulation of long noncoding RNA SPRY4-IT1 promotes metastasis of esophageal squamous cell carcinoma via induction of epithelial-mesenchymal transition.
abstract: Esophageal squamous cell carcinoma (ESCC) is one of the prevalent and deadly cancers worldwide, especially in Eastern Asia. Recent studies show that long noncoding RNAs (lncRNAs) have critical roles in diverse biological processes, including tumorigenesis. In the present study, we find that the expression of lncRNA SPRY4-IT1 is significantly upregulated in ESCC cell lines as compared with human esophageal epithelial cell line HEEC. Overexpression of SPRY4-IT1 can increase in vitro motility of ESCC cells via induction of epithelial-mesenchymal transition (EMT), which is characterized by increasing the expression of vimentin (Vim) and fibronectin (FN) with a concomitant decrease of E-cadherin (E-Cad) and ZO-1, while silencing of SPRY4-IT1 significantly inhibits the in vitro motility of ESCC cells. Further, the knockdown of SPRY4-IT1 also significantly attenuates TFG-β-induced EMT of ESCC cells. Further, lncRNA SPRY4-IT1 can directly increase the transcription, expression, and nuclear localization of Snail, one key transcription factor during the EMT processes of cancer cells, while siRNA-mediated specific knockdown of Snail can significantly attenuate SPRY4-IT1-induced EMT of ESCC cells. Our results suggest that lncRNA SPRY4-IT1 might be considered as a novel oncogene involved in ESCC progression.
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital, Zhengzhou University, No. 1 Jianshe East Road, Zhengzhou, Henan, 450052, China.|Open Key Clinical Medical Experimental Laboratory Institute of Henan Province, Zhengzhou, Henan, 450052, China.|Diagnosis and Treatment of Lung Cancer Research Center, Zhengzhou University, Zhengzhou, Henan, 450052, China.|Key Laboratory of Thoracic Tumors of Zhengzhou, Zhengzhou, Henan, 450052, China.
authors.forename: Chun-Yang
authors.initials: CY
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology, Orthopaedic Hospital of Zhengzhou, Zhengzhou, Henan, 450052, China.
authors.forename: Ren-Ke
authors.initials: RK
authors.lastname: Li
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital, Zhengzhou University, No. 1 Jianshe East Road, Zhengzhou, Henan, 450052, China.|Open Key Clinical Medical Experimental Laboratory Institute of Henan Province, Zhengzhou, Henan, 450052, China.|Diagnosis and Treatment of Lung Cancer Research Center, Zhengzhou University, Zhengzhou, Henan, 450052, China.|Key Laboratory of Thoracic Tumors of Zhengzhou, Zhengzhou, Henan, 450052, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Qi
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital, Zhengzhou University, No. 1 Jianshe East Road, Zhengzhou, Henan, 450052, China.|Open Key Clinical Medical Experimental Laboratory Institute of Henan Province, Zhengzhou, Henan, 450052, China.|Diagnosis and Treatment of Lung Cancer Research Center, Zhengzhou University, Zhengzhou, Henan, 450052, China.|Key Laboratory of Thoracic Tumors of Zhengzhou, Zhengzhou, Henan, 450052, China.
authors.forename: Xiang-Nan
authors.initials: XN
authors.lastname: Li
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital, Zhengzhou University, No. 1 Jianshe East Road, Zhengzhou, Henan, 450052, China.|Open Key Clinical Medical Experimental Laboratory Institute of Henan Province, Zhengzhou, Henan, 450052, China.|Diagnosis and Treatment of Lung Cancer Research Center, Zhengzhou University, Zhengzhou, Henan, 450052, China.|Key Laboratory of Thoracic Tumors of Zhengzhou, Zhengzhou, Henan, 450052, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital, Zhengzhou University, No. 1 Jianshe East Road, Zhengzhou, Henan, 450052, China.|Open Key Clinical Medical Experimental Laboratory Institute of Henan Province, Zhengzhou, Henan, 450052, China.|Diagnosis and Treatment of Lung Cancer Research Center, Zhengzhou University, Zhengzhou, Henan, 450052, China.|Key Laboratory of Thoracic Tumors of Zhengzhou, Zhengzhou, Henan, 450052, China.
authors.forename: Dong-Lei
authors.initials: DL
authors.lastname: Liu
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital, Zhengzhou University, No. 1 Jianshe East Road, Zhengzhou, Henan, 450052, China.|Open Key Clinical Medical Experimental Laboratory Institute of Henan Province, Zhengzhou, Henan, 450052, China.|Diagnosis and Treatment of Lung Cancer Research Center, Zhengzhou University, Zhengzhou, Henan, 450052, China.|Key Laboratory of Thoracic Tumors of Zhengzhou, Zhengzhou, Henan, 450052, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital, Zhengzhou University, No. 1 Jianshe East Road, Zhengzhou, Henan, 450052, China.|Open Key Clinical Medical Experimental Laboratory Institute of Henan Province, Zhengzhou, Henan, 450052, China.|Diagnosis and Treatment of Lung Cancer Research Center, Zhengzhou University, Zhengzhou, Henan, 450052, China.|Key Laboratory of Thoracic Tumors of Zhengzhou, Zhengzhou, Henan, 450052, China.
authors.forename: Deng-Yan
authors.initials: DY
authors.lastname: Zhu
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital, Zhengzhou University, No. 1 Jianshe East Road, Zhengzhou, Henan, 450052, China.|Open Key Clinical Medical Experimental Laboratory Institute of Henan Province, Zhengzhou, Henan, 450052, China.|Diagnosis and Treatment of Lung Cancer Research Center, Zhengzhou University, Zhengzhou, Henan, 450052, China.|Key Laboratory of Thoracic Tumors of Zhengzhou, Zhengzhou, Henan, 450052, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Wu
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital, Zhengzhou University, No. 1 Jianshe East Road, Zhengzhou, Henan, 450052, China.|Open Key Clinical Medical Experimental Laboratory Institute of Henan Province, Zhengzhou, Henan, 450052, China.|Diagnosis and Treatment of Lung Cancer Research Center, Zhengzhou University, Zhengzhou, Henan, 450052, China.|Key Laboratory of Thoracic Tumors of Zhengzhou, Zhengzhou, Henan, 450052, China.
authors.forename: Xu-Dong
authors.initials: XD
authors.lastname: Zhou
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital, Zhengzhou University, No. 1 Jianshe East Road, Zhengzhou, Henan, 450052, China. zhaosongzzucn@163.com.|Open Key Clinical Medical Experimental Laboratory Institute of Henan Province, Zhengzhou, Henan, 450052, China. zhaosongzzucn@163.com.|Diagnosis and Treatment of Lung Cancer Research Center, Zhengzhou University, Zhengzhou, Henan, 450052, China. zhaosongzzucn@163.com.|Key Laboratory of Thoracic Tumors of Zhengzhou, Zhengzhou, Henan, 450052, China. zhaosongzzucn@163.com.
authors.forename: Song
authors.initials: S
authors.lastname: Zhao
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D000077277:Esophageal Squamous Cell Carcinoma; D005786:Gene Expression Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism*; D015533:Transcriptional Activation; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; D062085:RNA, Long Noncoding; C000605823:long noncoding RNA SPRY4-IT1, human
keywords: EMT; ESCC; Metastasis; SPRY4-IT1; lncRNA