title: Functionalized nanoparticles based solid-phase membrane micro-tip extraction and high-performance liquid chromatography analyses of vitamin B complex in human plasma.
abstract: Iron nanoparticles were prepared by a green method following functionalization using 1-butyl-3-methylimidazolium bromide. 1-Butyl-3-methylimidazole iron nanoparticles were characterized using FTIR spectroscopy, energy dispersive X-ray fluorescence, X-ray diffraction, scanning electron microscopy and transmission electron microscopy. The nanoparticles were used in solid-phase membrane micro-tip extraction to separate vitamin B complex from plasma before high-performance liquid chromatography. The optimum conditions obtained were sorbent (15 mg), agitation time (30 min), pH (9.0), desorbing solvent [water (5 mL) + methanol (5 mL) + sodium hydroxide (0.1 N) + acetic acid (d = 1.05 kg/L, pH 5.5), desorbing volume (10 mL) and desorption time (30 min). The percentage recoveries of all the eight vitamin B complex were from 60 to 83%. A high-performance liquid chromatography method was developed using a PhE column (250 × 4.6 mm, 5.0 μm) and water/acetonitrile (95:5, v/v; pH 4.0 with 0.1% formic acid) mobile phase. The flow rate was 1.0 mL/min with detection at 270 and 210 nm. The values of the capacity, separation and resolution factor were 0.57-39.47, 1.12-6.00 and 1.84-26.26, respectively. The developed sample preparation and chromatographic methods were fast, selective, inexpensive, economic and reproducible. The developed method can be applied for analyzing these drugs in biological and environmental matrices.
authors.affiliation: Department of Chemistry, Jamia Millia Islamia, New Delhi, India.
authors.forename: Imran
authors.initials: I
authors.lastname: Ali
authors.affiliation: Department of Chemistry, Jamia Millia Islamia, New Delhi, India.
authors.forename: Umma
authors.initials: U
authors.lastname: Kulsum
authors.affiliation: Department of Chemistry, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Zeid A
authors.initials: ZA
authors.lastname: Al-Othman
authors.affiliation: Department of Chemistry, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Abdulrahman
authors.initials: A
authors.lastname: Alwarthan
authors.affiliation: Department of Chemistry, Jamia Millia Islamia, New Delhi, India.
authors.forename: Kishwar
authors.initials: K
authors.lastname: Saleem
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007501:Iron / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D052617:Solid Phase Microextraction; D014803:Vitamin B Complex / Q000097:blood*
chemical_list: D014803:Vitamin B Complex; D007501:Iron
keywords: Functionalized nanoparticles; High-performance liquid chromatography; Human plasma; Solid-phase membrane micro-tip extraction; Vitamin B complex