title: Generation of Mouse Monoclonal Antibodies Specific to Chikungunya Virus Using ClonaCell-HY Hybridoma Cloning Kit.
abstract: Monoclonal antibodies offer high specificity and this makes it an important tool for molecular biology, biochemistry and medicine. Typically, monoclonal antibodies are generated by fusing mouse spleen cells that have been immunized with the desired antigen with myeloma cells to create immortalized hybridomas. Here, we describe the generation of monoclonal antibodies that are specific to Chikungunya virus using ClonaCell-HY system.
authors.affiliation: Monoclonal Antibody Unit, Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research, 61 Biopolis Drive, Singapore, 138673, Singapore.
authors.forename: Chow Wenn
authors.initials: CW
authors.lastname: Yew
authors.affiliation: Monoclonal Antibody Unit, Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research, 61 Biopolis Drive, Singapore, 138673, Singapore. mcbtanyj@imcb.a-star.edu.sg.|Department of Microbiology, Yong Loo Lin School of Medicine, National University Health System (NUHS), National University of Singapore, 5 Science Drive 2, Blk MD4, Level 3, Singapore, 117545, Singapore. mcbtanyj@imcb.a-star.edu.sg.
authors.forename: Yee Joo
authors.initials: YJ
authors.lastname: Tan
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D000956:Antigens, Viral / Q000276:immunology*; D002460:Cell Line; D002646:Chikungunya virus / Q000276:immunology*; D007114:Immunization; D051379:Mice; D013154:Spleen / Q000166:cytology / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000956:Antigens, Viral
keywords: Chikungunya virus; Fusion; Immunofluorescence; Monoclonal antibody; Screening