title: Identification of Two bZIP Transcription Factors Interacting with the Promoter of Soybean Rubisco Activase Gene (GmRCAα).
abstract: Rubisco activase (RCA), a key photosynthetic protein, catalyses the activation of Rubisco and thus plays an important role in photosynthesis. Although the RCA gene has been characterized in a variety of species, the molecular mechanism regulating its transcription remains unclear. Our previous studies on RCA gene expression in soybean suggested that expression of this gene is regulated by trans-acting factors. In the present study, we verified activity of the GmRCAα promoter in both soybean and Arabidopsis and used a yeast one-hybrid (Y1H) system for screening a leaf cDNA expression library to identify transcription factors (TFs) interacting with the GmRCAα promoter. Four basic leucine zipper (bZIP) TFs, GmbZIP04g, GmbZIP07g, GmbZIP1, and GmbZIP71, were isolated, and GmbZIP04g and GmbZIP07g were confirmed as able to bind to a 21-nt G-box-containing sequence. Additionally, the expression patterns of GmbZIP04g, GmbZIp07g, and GmRCAα were analyzed in response to abiotic stresses and during a 24-h period. Our study will help to advance elucidation of the network regulating GmRCAα transcription.
authors.affiliation: National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University Nanjing, China.
authors.forename: Jinyu
authors.initials: J
authors.lastname: Zhang
authors.affiliation: National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University Nanjing, China.
authors.forename: Hongyang
authors.initials: H
authors.lastname: Du
authors.affiliation: Henan Collaborative Innovation Center of Modern Biological Breeding, Henan Institute of Science and Technology Xinxiang, China.
authors.forename: Maoni
authors.initials: M
authors.lastname: Chao
authors.affiliation: Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou University Yangzhou, China.
authors.forename: Zhitong
authors.initials: Z
authors.lastname: Yin
authors.affiliation: National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University Nanjing, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yang
authors.affiliation: National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University Nanjing, China.
authors.forename: Yakai
authors.initials: Y
authors.lastname: Li
authors.affiliation: National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University Nanjing, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Huang
authors.affiliation: National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University Nanjing, China.
authors.forename: Deyue
authors.initials: D
authors.lastname: Yu
keywords: Rubisco activase; basic leucine zipper; promoter; soybean; transcription factor