title: Bone healing capabilities of recombinant human bone morphogenetic protein-9 (rhBMP-9) with a chitosan or collagen carrier in rat calvarial defects.
abstract: The aim of this study was to examine the effects of recombinant human BMP-9 (rhBMP-9) with chitosan sponge (ChiS) or absorbable collagen sponge (ACS) on bone formation in rat calvarial defects. The defects were treated by one of the following implantations: ChiS, rhBMP-9/ChiS, ACS, rhBMP-9/ACS and no implantation. The animals were euthanized at 8 weeks for histological evaluation. The percentage of defect closure (DC) in the rhBMP-9/ACS group was significantly greater than that in the ACS group. The rhBMP-9/ACS group demonstrated the highest level of DC among all the groups. The newly formed bone area (NBA) and NBA/total area in the ChiS-implanted groups and in the rhBMP-9/ACS group were significantly greater compared with those in the ACS group. It can be concluded that rhBMP-9/ACS has a potential to induce bone formation in rat calvarial defects. Further studies are required to elucidate the mechanism of bone formation induced by rhBMP-9.
authors.affiliation: Department of Periodontology, Kagoshima University Graduate School of Medical and Dental Sciences.
authors.forename: Yukiya
authors.initials: Y
authors.lastname: Shinohara
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Nakamura
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Shirakata
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Noguchi
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D001861:Bone Regeneration; D048271:Chitosan; D003094:Collagen; D004337:Drug Carriers; D055427:Growth Differentiation Factor 2; D055412:Growth Differentiation Factors; D006801:Humans; D051381:Rats; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta
chemical_list: D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D004337:Drug Carriers; C525887:GDF2 protein, human; D055427:Growth Differentiation Factor 2; D055412:Growth Differentiation Factors; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D003094:Collagen; D048271:Chitosan