title: Influence of fluid dynamic conditions on enzymatic hydrolysis of lignocellulosic biomass: Effect of mass transfer rate.
abstract: The effect of fluid dynamic conditions on enzymatic hydrolysis of acid pretreated corn stover (PCS) has been assessed. Runs were performed in stirred tanks at several stirrer speed values, under typical conditions of temperature (50°C), pH (4.8) and solid charge (20% w/w). A complex mixture of cellulases, xylanases and mannanases was employed for PCS saccharification. At low stirring speeds (<150rpm), estimated mass transfer coefficients and rates, when compared to chemical hydrolysis rates, lead to results that clearly show low mass transfer rates, being this phenomenon the controlling step of the overall process rate. However, for stirrer speed from 300rpm upwards, the overall process rate is controlled by hydrolysis reactions. The ratio between mass transfer and overall chemical reaction rates changes with time depending on the conditions of each run.
authors.affiliation: Chemical Engineering Deparment, Universidad Complutense de Madrid, Avda. Complutense s/n, 28040 Madrid, Spain.
authors.forename: Mateusz
authors.initials: M
authors.lastname: Wojtusik
authors.affiliation: Abengoa Research, Campus de Palmas Altas, 41014 Sevilla, Spain.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Zurita
authors.affiliation: Laboratory of Cellulose and Paper, INIA, Forest Research Center, Ctra. de la Coruña km 7.5, 28040 Madrid, Spain.
authors.forename: Juan C
authors.initials: JC
authors.lastname: Villar
authors.affiliation: Chemical Engineering Deparment, Universidad Complutense de Madrid, Avda. Complutense s/n, 28040 Madrid, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: Ladero
authors.affiliation: Chemical Engineering Deparment, Universidad Complutense de Madrid, Avda. Complutense s/n, 28040 Madrid, Spain. Electronic address: fgochoa@ucm.es.
authors.forename: Felix
authors.initials: F
authors.lastname: Garcia-Ochoa
mesh_terms: D018533:Biomass; D002482:Cellulose / Q000737:chemistry; D057446:Hydrodynamics; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry*; D012037:Refuse Disposal / Q000379:methods*; D003313:Zea mays / Q000737:chemistry*
chemical_list: D002482:Cellulose; D008031:Lignin
keywords: Enzymatic hydrolysis; Fluid dynamic; Lignocellulosic biomass; Mass transfer; Stirrer speed