title: Assessment of the anti-metastatic properties of sanguiin H-6 in HUVECs and MDA-MB-231 human breast cancer cells.
abstract: The anti-metastatic properties of sanguiin H-6 were examined in human umbilical vein vascular endothelial cells (HUVECs) and MDA-MB-231 human breast cancer cells. In HUVECs, sanguiin H-6 inhibited the density of migrated cells compared to that observed after treatment with the vehicle. In addition, sanguiin H-6 at a concentration of 6.25μM significantly blocked tube formation. Treatment with up to 25μM sanguiin H-6 had no effect on MDA-MB-231 cells, whereas treatment with 200μM sanguiin H-6 decreased cell viability. Sanguiin H-6 significantly decreased the expression levels of vascular endothelial growth factor (VEGF), phosphorylated Akt, and extracellular signal-regulated kinase 1/2 (ERK1/2) in MDA-MB-231 cells. These findings suggest that sanguiin H-6 is potentially useful as an anti-metastatic agent.
authors.affiliation: Department of Surgery, University of Ulsan College of Medicine, Gangneung 210-711, Republic of Korea.
authors.forename: Eun-Hwa
authors.initials: EH
authors.lastname: Park
authors.affiliation: College of Korean Medicine, Gachon University, Seongnam 461-701, Republic of Korea.
authors.forename: Jun Yeon
authors.initials: JY
authors.lastname: Park
authors.affiliation: East West Cancer Center, Dunsan Korean Medical Hospital of Daejeon University, Daejeon 302-869, Republic of Korea.
authors.forename: Hwa-Seung
authors.initials: HS
authors.lastname: Yoo
authors.affiliation: Department of Obstetrics and Gynaecology, College of Korean Medicine, Daejeon University, Daejeon 300-716, Republic of Korea. Electronic address: jeyoo@dju.ac.kr.
authors.forename: Jeong-Eun
authors.initials: JE
authors.lastname: Yoo
authors.affiliation: College of Korean Medicine, Gachon University, Seongnam 461-701, Republic of Korea. Electronic address: hanilim@gachon.ac.kr.
authors.forename: Hye Lim
authors.initials: HL
authors.lastname: Lee
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005260:Female; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects*; D006801:Humans; D047348:Hydrolyzable Tannins / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D015394:Molecular Structure; D009362:Neoplasm Metastasis / Q000188:drug therapy / Q000473:pathology; D013329:Structure-Activity Relationship
chemical_list: D000970:Antineoplastic Agents; D047348:Hydrolyzable Tannins; C081844:sanguiin H 6
keywords: Breast cancer; HUVEC; Metastasis; Sanguiin H-6; VEGF