title: Expression of PAX8 Target Genes in Papillary Thyroid Carcinoma.
abstract: PAX8 is a thyroid-specific transcription factor whose expression is dysregulated in thyroid cancer. A recent study using a conditional knock-out mouse model identified 58 putative PAX8 target genes. In the present study, we evaluated the expression of 11 of these genes in normal and tumoral thyroid tissues from patients with papillary thyroid cancer (PTC). ATP1B1, GPC3, KCNIP3, and PRLR transcript levels in tumor tissues were significantly lower in PTCs than in NT, whereas LCN2, LGALS1 and SCD1 expression was upregulated in PTC compared with NT. Principal component analysis of the expression of the most markedly dysregulated PAX8 target genes was able to discriminate between PTC and NT. Immunohistochemistry was used to assess levels of proteins encoded by the two most dyregulated PAX8 target genes, LCN2 and GPC3. Interestingly, GPC3 was detectable in all of the NT samples but none of the PTC samples. Collectively, these findings point to significant PTC-associated dysregulation of several PAX8 target genes, supporting the notion that PAX8-regulated molecular cascades play important roles during thyroid tumorigenesis.
authors.affiliation: Department of Internal Medicine and Medical Specialties, "Sapienza" University of Rome, 00161 Rome, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Rosignolo
authors.affiliation: Department of Internal Medicine and Medical Specialties, "Sapienza" University of Rome, 00161 Rome, Italy.
authors.forename: Marialuisa
authors.initials: M
authors.lastname: Sponziello
authors.affiliation: Department of Internal Medicine and Medical Specialties, "Sapienza" University of Rome, 00161 Rome, Italy.
authors.forename: Cosimo
authors.initials: C
authors.lastname: Durante
authors.affiliation: Department of Medical and Biological Sciences, University of Udine, 33100 Udine, Italy.
authors.forename: Cinzia
authors.initials: C
authors.lastname: Puppin
authors.affiliation: Department of Medical and Biological Sciences, University of Udine, 33100 Udine, Italy.
authors.forename: Catia
authors.initials: C
authors.lastname: Mio
authors.affiliation: Department of Medical and Biological Sciences, University of Udine, 33100 Udine, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Baldan
authors.affiliation: Department of Medical and Biological Sciences, University of Udine, 33100 Udine, Italy.|Department of Oncology, University Hospital "S. Maria della Misericordia", 33100 Udine, Italy.
authors.forename: Carla
authors.initials: C
authors.lastname: Di Loreto
authors.affiliation: Department of Health Sciences, University "Magna Graecia" of Catanzaro, 88100 Catanzaro, Italy.
authors.forename: Diego
authors.initials: D
authors.lastname: Russo
authors.affiliation: Department of Internal Medicine and Medical Specialties, "Sapienza" University of Rome, 00161 Rome, Italy.
authors.forename: Sebastiano
authors.initials: S
authors.lastname: Filetti
authors.affiliation: Department of Medical and Biological Sciences, University of Udine, 33100 Udine, Italy.|Department of Oncology, University Hospital "S. Maria della Misericordia", 33100 Udine, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Damante
mesh_terms: D000818:Animals; D002291:Carcinoma, Papillary / Q000235:genetics*; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D000072636:PAX8 Transcription Factor / Q000235:genetics*; D025341:Principal Component Analysis; D012333:RNA, Messenger / Q000235:genetics; D013964:Thyroid Neoplasms / Q000235:genetics*
chemical_list: D000072636:PAX8 Transcription Factor; C083325:PAX8 protein, human; D012333:RNA, Messenger