title: Improvement of ectoine productivity by using sugar transporter-overexpressing Halomonas elongata.
abstract: We successfully enhanced the productivity of ectoine with Halomonas elongata by improvement of the transport of sugar. First, we carried out screening for sugar transporters capable of improving glucose and xylose consumption. We found two transporters: b3657 from Escherichia coli, which is capable of improving glucose consumption, and HEO_0208 from H. elongata, which is capable of improving xylose consumption. Using transporter-overexpressing strains, the productivity of ectoine was improved. These results indicate that sugar consumption is important for efficient ectoine production. As result of phenotypic analysis of a HEO_0208 deletion strain, we discovered that HEO_0208 is the major xylose transporter in H. elongata. This is the first report demonstrating improvement of ectoine productivity by enhancing the transport of sugar.
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai-cho, Nada, Kobe 657-8501, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Tanimura
authors.affiliation: Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodai-cho, Nada, Kobe 657-8501, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Matsumoto
authors.affiliation: Division of Environmental Protection Technology, Institute of Environmental Science, Graduate School of Fisheries and Environmental Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Nakayama
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai-cho, Nada, Kobe 657-8501, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Tanaka
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai-cho, Nada, Kobe 657-8501, Japan. Electronic address: akondo@kobe-u.ac.jp.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
mesh_terms: D000599:Amino Acids, Diamino / Q000096:biosynthesis*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D005285:Fermentation; D017353:Gene Deletion; D005798:Genes, Bacterial; D051246:Glucose Transport Proteins, Facilitative / Q000235:genetics / Q000378:metabolism; D020632:Halomonas / Q000235:genetics / Q000378:metabolism*; D009004:Monosaccharide Transport Proteins / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D014994:Xylose / Q000378:metabolism
chemical_list: D000599:Amino Acids, Diamino; D001426:Bacterial Proteins; D051246:Glucose Transport Proteins, Facilitative; D009004:Monosaccharide Transport Proteins; C045628:ectoine; D014994:Xylose
keywords: Ectoine; Glucose; Halomonas elongata; Sugar transport; Xylose