title: Genetic Modification of Human Pancreatic Progenitor Cells Through Modified mRNA.
abstract: In this chapter, we describe a highly efficient genetic modification strategy for human pancreatic progenitor cells using modified mRNA-encoding GFP and Neurogenin-3. The properties of modified mRNA offer an invaluable platform to drive protein expression, which has broad applicability in pathway regulation, directed differentiation, and lineage specification. This approach can also be used to regulate expression of other pivotal transcription factors during pancreas development and might have potential therapeutic values in regenerative medicine.
authors.affiliation: Department of Chemical Pathology, The Chinese University of Hong Kong, Princes of Wales Hospital, Shatin, Hong Kong, SAR, China.|Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Princes of Wales Hospital, Shatin, Hong Kong, SAR, China.
authors.forename: Song
authors.initials: S
authors.lastname: Lu
authors.affiliation: Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Princes of Wales Hospital, Shatin, Hong Kong, SAR, China.
authors.forename: Christie C
authors.initials: CC
authors.lastname: Chow
authors.affiliation: School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong, SAR, China.
authors.forename: Junwei
authors.initials: J
authors.lastname: Zhou
authors.affiliation: School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong, SAR, China.
authors.forename: Po Sing
authors.initials: PS
authors.lastname: Leung
authors.affiliation: School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong, SAR, China.
authors.forename: Stephen K
authors.initials: SK
authors.lastname: Tsui
authors.affiliation: Department of Chemical Pathology, The Chinese University of Hong Kong, Princes of Wales Hospital, Shatin, Hong Kong, SAR, China. kathyolui@cuhk.edu.hk.|Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Princes of Wales Hospital, Shatin, Hong Kong, SAR, China. kathyolui@cuhk.edu.hk.
authors.forename: Kathy O
authors.initials: KO
authors.lastname: Lui
mesh_terms: D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics* / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D005818:Genetic Engineering; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology* / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics*; D011994:Recombinant Proteins / Q000378:metabolism; D014162:Transfection
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D049452:Green Fluorescent Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; C498695:NEUROG3 protein, human; C093564:Neurogenic differentiation factor 1
keywords: Gene therapy; Human pancreas development; Modified mRNA; Pancreatic progenitors; Regenerative medicine; Transcription factors