title: The yeast Hsp70 homolog Ssb: a chaperone for general de novo protein folding and a nanny for specific intrinsically disordered protein domains.
abstract: Activation of the heterotrimeric kinase SNF1 via phosphorylation of a specific residue within the α subunit is essential for the release from glucose repression in the yeast Saccharomyces cerevisiae. When glucose is available, SNF1 is maintained in the dephosphorylated, inactive state by the phosphatase Glc7-Reg1. Recent findings suggest that Bmh and Ssb combine their unique client-binding properties to interact with the regulatory region of the SNF1 α subunit and by that stabilize a conformation of SNF1, which is accessible for Glc7-Reg1-dependent dephosphorylation. Together, the 14-3-3 protein Bmh and the Hsp70 homolog Ssb comprise a novel chaperone module, which is required to maintain proper glucose repression in the yeast S. cerevisiae.
authors.affiliation: Faculty of Medicine, ZBMZ, Institute of Biochemistry and Molecular Biology, University of Freiburg, 79104, Freiburg, Germany.
authors.forename: Volker
authors.initials: V
authors.lastname: Hübscher
authors.affiliation: Faculty of Medicine, ZBMZ, Institute of Biochemistry and Molecular Biology, University of Freiburg, 79104, Freiburg, Germany.
authors.forename: Kaivalya
authors.initials: K
authors.lastname: Mudholkar
authors.affiliation: Faculty of Medicine, ZBMZ, Institute of Biochemistry and Molecular Biology, University of Freiburg, 79104, Freiburg, Germany. sabine.rospert@biochemie.uni-freiburg.de.|BIOSS Centre for Biological Signaling Studies, University of Freiburg, 79104, Freiburg, Germany. sabine.rospert@biochemie.uni-freiburg.de.
authors.forename: Sabine
authors.initials: S
authors.lastname: Rospert
mesh_terms: D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism*; D050884:HSP72 Heat-Shock Proteins / Q000737:chemistry / Q000378:metabolism*; D064267:Intrinsically Disordered Proteins / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D000072417:Protein Domains; D017510:Protein Folding; D054730:Protein Interaction Domains and Motifs; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry / Q000378:metabolism; D015003:Yeasts / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D050884:HSP72 Heat-Shock Proteins; D064267:Intrinsically Disordered Proteins; D029701:Saccharomyces cerevisiae Proteins
keywords: 14-3-3; AMPK; Bmh; Glc7; Hsp70; PKA; Reg1; SNF1; Saccharomyces cerevisiae; Ssb