title: GC Preps: Fast and Easy Extraction of Stable Yeast Genomic DNA.
abstract: Existing yeast genomic DNA extraction methods are not ideally suited to extensive screening of colonies by PCR, due to being too lengthy, too laborious or yielding poor quality DNA and inconsistent results. We developed the GC prep method as a solution to this problem. Yeast cells from colonies or liquid cultures are lysed by vortex mixing with glass beads and then boiled in the presence of a metal chelating resin. In around 12 minutes, multiple samples can be processed to extract high yields of genomic DNA. These preparations perform as effectively in PCR screening as DNA purified by organic solvent methods, are stable for up to 1 year at room temperature and can be used as the template for PCR amplification of fragments of at least 8 kb.
authors.affiliation: Centre for Synthetic Biology and Innovation, Imperial College London, London, United Kingdom.|Department of Bioengineering, Imperial College London, London, United Kingdom.
authors.forename: Benjamin A
authors.initials: BA
authors.lastname: Blount
authors.affiliation: Centre for Synthetic Biology and Innovation, Imperial College London, London, United Kingdom.|Department of Bioengineering, Imperial College London, London, United Kingdom.
authors.forename: Maureen R M
authors.initials: MR
authors.lastname: Driessen
authors.affiliation: Centre for Synthetic Biology and Innovation, Imperial College London, London, United Kingdom.|Department of Bioengineering, Imperial College London, London, United Kingdom.
authors.forename: Tom
authors.initials: T
authors.lastname: Ellis
mesh_terms: D004271:DNA, Fungal / Q000302:isolation & purification*; D016681:Genome, Fungal; D023281:Genomics; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D004271:DNA, Fungal