title: RIG-I-Like Receptors: One STrEP Forward.
abstract: RIG-I-like receptors detect cytosolic viral RNA and activate an antiviral innate immune response. A new study employs the one STrEP-purification technique and next generation sequencing to characterize physiological ligands in an infected cell. The view of all three RLRs bound to viral RNAs shows specialization, collaboration and new binding sites.
authors.affiliation: Gene Center, Ludwig-Maximilians-Universität München, Munich, Germany; Department of Biochemistry, Ludwig-Maximilians-Universität München, Munich, Germany.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Lässig
authors.affiliation: Gene Center, Ludwig-Maximilians-Universität München, Munich, Germany; Department of Biochemistry, Ludwig-Maximilians-Universität München, Munich, Germany; Center for Integrated Protein Science, Ludwig-Maximilians-Universität München, Munich, Germany. Electronic address: hopfner@genzentrum.lmu.de.
authors.forename: Karl-Peter
authors.initials: KP
authors.lastname: Hopfner
mesh_terms: D002460:Cell Line; D000071457:DEAD Box Protein 58 / Q000276:immunology*; D057809:HEK293 Cells; D006801:Humans; D007113:Immunity, Innate / Q000276:immunology*; D000072640:Interferon-Induced Helicase, IFIH1 / Q000276:immunology*; D012327:RNA Virus Infections / Q000175:diagnosis / Q000276:immunology*; D012367:RNA, Viral / Q000235:genetics; D011971:Receptors, Immunologic; D015398:Signal Transduction / Q000276:immunology
chemical_list: D012367:RNA, Viral; D011971:Receptors, Immunologic; C452820:RIGI protein, human; C452815:IFIH1 protein, human; D000071457:DEAD Box Protein 58; D000072640:Interferon-Induced Helicase, IFIH1
keywords: LGP2; MDA5; RIG-I; RNA; affinity purification; innate immune system; next generation sequencing