title: Nanocapsules with glycerol monolaurate: Effects on Candida albicans biofilms.
abstract: Candida albicans does not only occur in the free living planktonic form but also grows in surface-attached biofilm communities. Moreover, these biofilms appear to be the most common lifestyle and are involved in the majority of human Candida infections. Nanoparticles can be used as an alternative to conventional antimicrobial agents and can also act as carriers for antibiotics and other drugs. In view of this, the aim of the study was develop, characterize and verify the anti-biofilm potential of GML Nanocapsules against C. albicans. The GML Nanocapsules showed mean diameter of 193.2 nm, polydispersion index of 0.044, zeta potential of -23.3 mV and pH 6.32. The microdilution assay showed MIC of 15.5 μg mL(-1) to GML Nanocapsules and 31.25 μg mL(-1) to GML. The anti-biofilm assay showed the significantly reduction of biomass of C. albicans biofilm treated with GML Nanocapsules while the GML does not exhibit effect. The kinetic assay demonstrated that at 48 h, the GML Nanocapsules reduce 94% of formed biofilm. The positive results suggest the promisor alternative for this public health problem that is biofilm infections.
authors.affiliation: Laboratory of Microbiology Research, Centro Universitário Franciscano, Santa Maria, Brazil; Post Graduate Program in Nanosciences, Centro Universitário Franciscano, Santa Maria, Brazil. Electronic address: leonardoquintanalopes@gmail.com.
authors.forename: Leonardo Quintana Soares
authors.initials: LQ
authors.lastname: Lopes
authors.affiliation: Post Graduate Program in Nanosciences, Centro Universitário Franciscano, Santa Maria, Brazil.
authors.forename: Cayane Genro
authors.initials: CG
authors.lastname: Santos
authors.affiliation: Laboratory of Microbiology Research, Centro Universitário Franciscano, Santa Maria, Brazil; Post Graduate Program in Nanosciences, Centro Universitário Franciscano, Santa Maria, Brazil.
authors.forename: Rodrigo de Almeida
authors.initials: Rde A
authors.lastname: Vaucher
authors.affiliation: Post Graduate Program in Nanosciences, Centro Universitário Franciscano, Santa Maria, Brazil.
authors.forename: Renata Platcheck
authors.initials: RP
authors.lastname: Raffin
authors.affiliation: Laboratory of Microbiology Research, Centro Universitário Franciscano, Santa Maria, Brazil; Post Graduate Program in Nanosciences, Centro Universitário Franciscano, Santa Maria, Brazil; Microbiology and Parasitology Department, Health Sciences Center, Universidade Federal de Santa Maria, Santa Maria, Brazil.
authors.forename: Roberto Christ Vianna
authors.initials: RC
authors.lastname: Santos
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D002176:Candida albicans / Q000187:drug effects* / Q000502:physiology; D007848:Laurates / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D050178:Monoglycerides / Q000494:pharmacology*; D053769:Nanocapsules
chemical_list: D000935:Antifungal Agents; D007848:Laurates; D050178:Monoglycerides; D053769:Nanocapsules; C020777:monolaurin
keywords: Anti-biofilm; Candida albicans; Glycerol monolaurate; Kinetic; Nanocapsules