title: Determination of 2-Arachidonoylglycerol by μSPE-LC-MS/MS.
abstract: LC-MS/MS is a powerful analytical technique that provides unequivocal identification and reliable quantification of the analytes, using Selected Reaction Monitoring or Multi Reaction Monitoring acquisition mode.2-Arachidonoylglycerol (2-AG) is the most abundant endocannabinoid, which plays a major role in a wide variety of physiological and pathological processes. Analysis of 2-AG by means of LC-MS/MS allows the detection of very low concentrations in biological samples. Here, we describe how to determine 2-AG levels in tiny samples of tissues and plasma through LC-MS/MS, by using very quick and easy to perform extraction procedures, with reduced solvent consumption.
authors.affiliation: Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Via 1, Teramo, 64023, Italy.
authors.forename: Natalia
authors.initials: N
authors.lastname: Battista
authors.affiliation: Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Via 1, Teramo, 64023, Italy. msergi@unite.it.
authors.forename: Manuel
authors.initials: M
authors.lastname: Sergi
mesh_terms: D001095:Arachidonic Acids / Q000097:blood* / Q000737:chemistry* / Q000302:isolation & purification; D002853:Chromatography, Liquid; D063388:Endocannabinoids / Q000097:blood* / Q000737:chemistry* / Q000302:isolation & purification; D005989:Glycerides / Q000097:blood* / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D059625:Liquid-Liquid Extraction; D053719:Tandem Mass Spectrometry
chemical_list: D001095:Arachidonic Acids; D063388:Endocannabinoids; D005989:Glycerides; C094503:glyceryl 2-arachidonate
keywords: 2-AG; LC-MS/MS; Plasma; Tissue; μSPE