title: Combinatorial Histone Readout by the Dual Plant Homeodomain (PHD) Fingers of Rco1 Mediates Rpd3S Chromatin Recruitment and the Maintenance of Transcriptional Fidelity.
abstract: The plant homeodomain (PHD) finger is found in many chromatin-associated proteins and functions to recruit effector proteins to chromatin through its ability to bind both methylated and unmethylated histone residues. Here, we show that the dual PHD fingers of Rco1, a member of the Rpd3S histone deacetylase complex recruited to transcribing genes, operate in a combinatorial manner in targeting the Rpd3S complex to histone H3 in chromatin. Although mutations in either the first or second PHD finger allow for Rpd3S complex formation, the assembled complexes from these mutants cannot recognize nucleosomes or function to maintain chromatin structure and prevent cryptic transcriptional initiation from within transcribed regions. Taken together, our findings establish a critical role of combinatorial readout in maintaining chromatin organization and in enforcing the transcriptional fidelity of genes.
authors.affiliation: From the Curriculum in Genetics and Molecular Biology and.
authors.forename: Stephen L
authors.initials: SL
authors.lastname: McDaniel
authors.affiliation: the Northwestern University Feinberg School of Medicine, Chicago, Illinois 60611, and.
authors.forename: Jennifer E
authors.initials: JE
authors.lastname: Fligor
authors.affiliation: the Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390.
authors.forename: Chun
authors.initials: C
authors.lastname: Ruan
authors.affiliation: the Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390.
authors.forename: Haochen
authors.initials: H
authors.lastname: Cui
authors.affiliation: the Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599.
authors.forename: Joseph B
authors.initials: JB
authors.lastname: Bridgers
authors.affiliation: From the Curriculum in Genetics and Molecular Biology and.
authors.forename: Julia V
authors.initials: JV
authors.lastname: DiFiore
authors.affiliation: the Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599.
authors.forename: Angela H
authors.initials: AH
authors.lastname: Guo
authors.affiliation: the Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390.
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.affiliation: From the Curriculum in Genetics and Molecular Biology and the Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, brian_strahl@med.unc.edu.
authors.forename: Brian D
authors.initials: BD
authors.lastname: Strahl
mesh_terms: D000595:Amino Acid Sequence; D002843:Chromatin / Q000378:metabolism*; D006655:Histone Deacetylases / Q000378:metabolism; D006657:Histones / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
chemical_list: D002843:Chromatin; D006657:Histones; D010940:Plant Proteins; D006655:Histone Deacetylases
keywords: PHD finger; Rco1; Rpd3S; chromatin; gene transcription; histone acetylation; histone deacetylase (HDAC)