title: Proteomic Response and Quality Maintenance in Postharvest Fruit of Strawberry (Fragaria × ananassa) to Exogenous Cytokinin.
abstract: The limitations in current understanding of the molecular mechanisms underlying fruit response to the application of plant growth regulators have increasingly become major challenges in improvement of crop quality. This study aimed to evaluate the response of strawberry to the preharvest application of exogenous cytokinin known as forchlorfenuron (CPPU). Postharvest internal and physiological quality attributes were characterized following storage under different conditions. Hierarchical clustering analysis via a label-free proteomic quantitative approach identified a total of 124 proteins in strawberries across all treatments. The expression profiles of both proteins and genes spanned the ranged role of cytokinin involved in primary and secondary metabolism, stress response, and so on. Eighty-eight proteins and fifty-six proteins were significantly regulated immediately at harvest and after storage, respectively. In general, the glycolysis in strawberry was only regulated by CPPU before storage; in addition to the accelerated photosynthesis and acid metabolism, CPPU application maintained higher capacity of resistance in strawberry to stress stimuli after storage, in comparison to control. Nevertheless, the volatile biosynthesis in strawberry has been suppressed by exogenous CPPU. Novel cytokinin response proteins and processes were identified in addition to the main transcriptomic expression to gain insights into the phytohormone control of fruit postharvest quality.
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Li
authors.affiliation: College of Biosystems Engineering and Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors.forename: Zisheng
authors.initials: Z
authors.lastname: Luo
authors.affiliation: Key Laboratory of Food Nutrition and Safety (Ministry of Education), Tianjin University of Science and Technology, Tianjin 300457, People's Republic of China.
authors.forename: Xinhong
authors.initials: X
authors.lastname: Huang
authors.affiliation: Key Laboratory of Food Nutrition and Safety (Ministry of Education), Tianjin University of Science and Technology, Tianjin 300457, People's Republic of China.
authors.forename: Xihong
authors.initials: X
authors.lastname: Li
mesh_terms: D003583:Cytokinins / Q000494:pharmacology*; D031985:Fragaria / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D005638:Fruit / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D053858:Metabolic Networks and Pathways / Q000187:drug effects / Q000235:genetics; D058977:Molecular Sequence Annotation; D010671:Phenylurea Compounds / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D040901:Proteomics; D011725:Pyridines / Q000494:pharmacology*; D013997:Time Factors; D059467:Transcriptome
chemical_list: D003583:Cytokinins; D010671:Phenylurea Compounds; D010937:Plant Growth Regulators; D010940:Plant Proteins; D011725:Pyridines; C116093:N-(2-chloro-4-pyridyl)-N'-phenylurea