title: [Dynamic changes of TGF-α and TGF-β1 in rats with liver cirrhosis induced by multiple pathogenic factors].
abstract: OBJECTIVE
  To explore the dynamic changes of transforming growth factor-α (TGF-α) and transforming growth factor-β1 (TGF-β1) of liver cirrhosis induced by multiple pathogenic factors in rats.
  
  
  METHODS
  Animals in the cirrhosis group were fed a mixture of maize flour, lard, cholesterol and alcohol plus subcutaneously injection with carbon tetrachloride (CCl₄), the CCl₄(0.5 ml/100 g · w) was injected at the first day of experiment and the 40% CCl₄oil solution (0.3 ml /100 g · w) was injected at an interval of three days. The thirty-six male SD rats were randomly divided into liver cirrhosis group of the 4th, 6th and 8 th week, and normal control group of the 4th, 6th and 8th week. The contents of alanine transferase (ALT), endotoxin, tumor necrosis factor-α (TNF-α) and homocysteine (Hcy) in plasma were evaluated. Histopathological changes of the liver were observed under microscope with the staining of HE. The expressions of TGF-α and TGF-β1 were analyzed by the method of immunohistochemistry.
  
  
  RESULTS
  Compared with the corresponding normal control group, the levels of ALT, endotoxin, TNF-α and Hcy in plasma were gradually significantly increased in liver cirrhosis group of the 4th, 6th and 8th week (P < 0.05); the expression of TGF-α in the liver tissues was significantly increased at the 4th week (P < 0.05); the expression of TGF-β1 in the liver tissues was gradually significantly increased in every model group (P < 0.05).
  
  
  CONCLUSIONS
  In the formation process of cirrhosis, the expression of TGF-α was increased in liver of cirrhosis group at the 4th week, and later it was suppressed; the expression of TGF-β1 was continuously increased. The characteristic dynamic changes of TGF-α and TGF-β1 might be related to sustained endotoxemia, the high level of TNF-α and hyperhomocysteinemia.
authors.forename: Xiao-xia
authors.initials: XX
authors.lastname: Tian
authors.forename: Hui-ying
authors.initials: HY
authors.lastname: Zhang
authors.forename: Li-min
authors.initials: LM
authors.lastname: Wang
authors.forename: Xu-jiong
authors.initials: XJ
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Li-li
authors.initials: LL
authors.lastname: Zhang
authors.forename: Yang-hui
authors.initials: YH
authors.lastname: Bi
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D002251:Carbon Tetrachloride; D004731:Endotoxins / Q000097:blood; D006710:Homocysteine / Q000097:blood; D008103:Liver Cirrhosis / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D016211:Transforming Growth Factor alpha / Q000378:metabolism*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000378:metabolism
chemical_list: D004731:Endotoxins; C508962:Tgfb1 protein, rat; D016211:Transforming Growth Factor alpha; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha; D006710:Homocysteine; D002251:Carbon Tetrachloride; D000410:Alanine Transaminase