title: Analysis of a new cluster of genes involved in the synthesis of the unique volatile organic compound sodorifen of Serratia plymuthica 4Rx13.
abstract: The rhizobacterium Serratia plymuthica 4Rx13 emits the novel and unique volatile sodorifen (C16H26), which has a polymethylated bicyclic structure. Transcriptome analysis revealed that gene SOD_c20750 (annotated as terpene cyclase) is involved in the biosynthesis of sodorifen. Here we show that this gene is located in a small cluster of four genes (SOD_c20750 - SOD_c20780), and the analysis of the knockout mutants demonstrated that SOD_c20760 (annotated as methyltransferase) and SOD_c20780 (annotated as isopentenyl pyrophosphate (IPP) isomerase) are needed for the biosynthesis of sodorifen, while a sodorifen-negative phenotype was not achieved with the SOD_c20770 (annotated as deoxy-xylulose-5-phosphate (DOXP) synthase) mutant. Altogether, the function of this new gene cluster was assigned to the biosynthesis of this structurally unusual volatile compound sodorifen.
authors.affiliation: Institute for Biological Sciences, University of Rostock, Albert-Einstein-Strasse 3, 18059 Rostock, Germany.
authors.forename: Dajana
authors.initials: D
authors.lastname: Domik
authors.affiliation: Institute for Biological Sciences, University of Rostock, Albert-Einstein-Strasse 3, 18059 Rostock, Germany.
authors.forename: Nancy
authors.initials: N
authors.lastname: Magnus
authors.affiliation: Institute for Biological Sciences, University of Rostock, Albert-Einstein-Strasse 3, 18059 Rostock, Germany birgit.piechulla@uni-rostock.de.
authors.forename: Birgit
authors.initials: B
authors.lastname: Piechulla
mesh_terms: D001643:Bridged Bicyclo Compounds / Q000378:metabolism*; D020869:Gene Expression Profiling; D055786:Gene Knockout Techniques; D023061:Gene Order; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D005810:Multigene Family; D009795:Octanes / Q000378:metabolism*; D012705:Serratia / Q000235:genetics* / Q000378:metabolism*; D059467:Transcriptome; D055549:Volatile Organic Compounds / Q000378:metabolism*
chemical_list: C549933:1,2,4,5,6,7,8-heptamethyl-3-methylenebicyclo(3.2.1)oct-6-ene; D001643:Bridged Bicyclo Compounds; D009795:Octanes; D055549:Volatile Organic Compounds
keywords: Serratia plymuthica; bacterial DOXP synthase; bacterial methyltransferase; bacterial terpene cyclase; sodorifen; volatile organic compound (VOC)