title: Anesthetic constituents of Zanthoxylum bungeanum Maxim.: A pharmacokinetic study.
abstract: A sensitive and selective ultra high performance liquid chromatography with tandem mass spectrometry method was established and validated for the simultaneous determination of hydroxy-α-sanshool, hydroxy-β-sanshool, and hydroxy-γ-sanshool in rat plasma after the subcutaneous and intravenous administration of an extract of the pericarp of Zanthoxylum bungeanum Maxim. Piperine was used as the internal standard. The analytes were extracted from rat plasma by liquid-liquid extraction with ethyl acetate and separated on a Thermo Hypersil GOLD C18 column (2.1 mm × 50 mm, 1.9 μm) with a gradient elution system at a flow rate of 0.4 mL/min. The mobile phase consisted of acetonitrile/0.05% formic acid in water and the total analysis time was 4 min. Positive electrospray ionization was performed using multiple reaction monitoring mode for the analytes. The calibration curves of the three analytes were linear over the tested concentration range. The intra- and interday precision was no more than 13.6%. Extraction recovery, matrix effect, and stability were satisfactory in rat plasma. The developed and validated method was suitable for the quantification of hydroxy-α-sanshool, hydroxy-β-sanshool, and hydroxy-γ-sanshool and successfully applied to a pharmacokinetic study of these analytes after subcutaneous and intravenous administration to rats.
authors.affiliation: Institute Department of Pharmaceutical Analysis, School of Pharmacy,  Shenyang Pharmaceutical University, Shenyang, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Rong
authors.affiliation: Institute Department of Pharmaceutical Analysis, School of Pharmacy,  Shenyang Pharmaceutical University, Shenyang, China.
authors.forename: Mei-Yu
authors.initials: MY
authors.lastname: Cui
authors.affiliation: Institute Department of Pharmaceutical Analysis, School of Pharmacy,  Shenyang Pharmaceutical University, Shenyang, China.
authors.forename: Qi-Li
authors.initials: QL
authors.lastname: Zhang
authors.affiliation: Institute Department of Pharmaceutical Analysis, School of Pharmacy,  Shenyang Pharmaceutical University, Shenyang, China.
authors.forename: Mei-Yan
authors.initials: MY
authors.lastname: Zhang
authors.affiliation: Institute Department of Pharmaceutical Analysis, School of Pharmacy,  Shenyang Pharmaceutical University, Shenyang, China.
authors.forename: Yu-Ming
authors.initials: YM
authors.lastname: Yu
authors.affiliation: Institute Department of Pharmaceutical Analysis, School of Pharmacy,  Shenyang Pharmaceutical University, Shenyang, China.
authors.forename: Xian-Ying
authors.initials: XY
authors.lastname: Zhou
authors.affiliation: Institute Department of Pharmaceutical Analysis, School of Pharmacy,  Shenyang Pharmaceutical University, Shenyang, China.
authors.forename: Zhi-Guo
authors.initials: ZG
authors.lastname: Yu
authors.affiliation: Institute Department of Pharmaceutical Analysis, School of Pharmacy,  Shenyang Pharmaceutical University, Shenyang, China.
authors.forename: Yun-Li
authors.initials: YL
authors.lastname: Zhao
mesh_terms: D000577:Amides / Q000032:analysis / Q000493:pharmacokinetics*; D000777:Anesthetics / Q000032:analysis / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid; D059625:Liquid-Liquid Extraction; D015394:Molecular Structure; D053719:Tandem Mass Spectrometry; D032106:Zanthoxylum / Q000737:chemistry*
chemical_list: D000577:Amides; D000777:Anesthetics; C100574:sanshool
keywords: Pharmacokinetics; Sanshools; Traditional Chinese medicine; Zanthoxylum bungeanum Maxim