title: Domestic wastewater treatment with purple phototrophic bacteria using a novel continuous photo anaerobic membrane bioreactor.
abstract: A key future challenge of domestic wastewater treatment is nutrient recovery while still achieving acceptable discharge limits. Nutrient partitioning using purple phototrophic bacteria (PPB) has the potential to biologically concentrate nutrients through growth. This study evaluates the use of PPB in a continuous photo-anaerobic membrane bioreactor (PAnMBR) for simultaneous organics and nutrient removal from domestic wastewater. This process could continuously treat domestic wastewater to discharge limits (<50 mgCOD L(-1), 5 mgN L(-1), 1.0 mgP L(-1)). Approximately 6.4 ± 1.3 gNH4-N and 1.1 ± 0.2 gPO4-P for every 100 gSCOD were removed at a hydraulic retention time of 8-24 h and volumetric loading rates of 0.8-2.5 COD kg m(3) d(-1). Thus, a minimum of 200 mg L(-1) of ethanol (to provide soluble COD) was required to achieve these discharge limits. Microbial community through sequencing indicated dominance of >60% of PPB, though the PPB community was highly variable. The outcomes from the current work demonstrate the potential of PPB for continuous domestic (and possibly industrial) wastewater treatment and nutrient recovery. Technical challenges include the in situ COD supply in a continuous reactor system, as well as efficient light delivery. Addition of external (agricultural or fossil) derived organics is not financially nor environmentally justified, and carbon needs to be sourced internally from the biomass itself to enable this technology. Reduced energy consumption for lighting is technically feasible, and needs to be addressed as a key objective in scaleup.
authors.affiliation: Advanced Water Management Centre, Gehrmann Building, The University of Queensland, Brisbane, Queensland 4072, Australia; CRC for Water Sensitive Cities, PO Box 8000, Clayton, Victoria 3800, Australia. Electronic address: t.huelsen@awmc.uq.edu.au.
authors.forename: Tim
authors.initials: T
authors.lastname: Hülsen
authors.affiliation: Advanced Water Management Centre, Gehrmann Building, The University of Queensland, Brisbane, Queensland 4072, Australia.
authors.forename: Edward M
authors.initials: EM
authors.lastname: Barry
authors.affiliation: Advanced Water Management Centre, Gehrmann Building, The University of Queensland, Brisbane, Queensland 4072, Australia; CRC for Water Sensitive Cities, PO Box 8000, Clayton, Victoria 3800, Australia.
authors.forename: Yang
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Advanced Water Management Centre, Gehrmann Building, The University of Queensland, Brisbane, Queensland 4072, Australia; CRC for Water Sensitive Cities, PO Box 8000, Clayton, Victoria 3800, Australia.
authors.forename: Daniel
authors.initials: D
authors.lastname: Puyol
authors.affiliation: Advanced Water Management Centre, Gehrmann Building, The University of Queensland, Brisbane, Queensland 4072, Australia; CRC for Water Sensitive Cities, PO Box 8000, Clayton, Victoria 3800, Australia.
authors.forename: Jürg
authors.initials: J
authors.lastname: Keller
authors.affiliation: Advanced Water Management Centre, Gehrmann Building, The University of Queensland, Brisbane, Queensland 4072, Australia; CRC for Water Sensitive Cities, PO Box 8000, Clayton, Victoria 3800, Australia.
authors.forename: Damien J
authors.initials: DJ
authors.lastname: Batstone
mesh_terms: D001419:Bacteria; D001421:Bacteria, Anaerobic; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D002244:Carbon; D014865:Waste Disposal, Fluid; D062065:Wastewater / Q000382:microbiology*
chemical_list: D002244:Carbon; D062065:Wastewater
keywords: COD; Domestic wastewater; Nutrient recovery; Purple phototrophic bacteria