title: Analysis of gene functions in Maize chlorotic mottle virus.
abstract: Gene functions of strains of Maize chlorotic mottle virus, which comprises the monotypic genus Machlomovirus, have not been previously identified. In this study mutagenesis of the seven genes encoded in maize chlorotic mottle virus (MCMV) showed that the genes with positional and sequence similarity to their homologs in viruses of related tombusvirid genera had similar functions. p50 and its readthrough protein p111 are the only proteins required for replication in maize protoplasts, and they function at a low level in trans. Two movement proteins, p7a and p7b, and coat protein, encoded on subgenomic RNA1, are required for cell-to-cell movement in maize, and p7a and p7b function in trans. A unique protein, p31, expressed as a readthrough extension of p7a, is required for efficient systemic infection. The 5' proximal MCMV gene encodes a unique 32kDa protein that is not required for replication or movement. Transcripts lacking p32 expression accumulate to about 1/3 the level of wild type transcripts in protoplasts and produce delayed, mild infections in maize plants. Additional studies on p32, p31 and the unique amino-terminal region of p50 are needed to further characterize the life cycle of this unique tombusvirid.
authors.affiliation: Department of Plant Biology, Ecology, and Evolution, 301 Physical Sciences, Oklahoma State University, Stillwater, OK, 74078-3013, USA. Electronic address: kay.scheets@okstate.edu.
authors.forename: Kay
authors.initials: K
authors.lastname: Scheets
mesh_terms: D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D009154:Mutation; D016366:Open Reading Frames; D010935:Plant Diseases / Q000821:virology; D011523:Protoplasts; D012367:RNA, Viral; D019183:Tombusviridae / Q000502:physiology*; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D014779:Virus Replication; D003313:Zea mays / Q000821:virology*
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins
keywords: Gene function; Machlomovirus; Overprinted gene; RNA virus replication; Tombusviridae; Viral movement