title: In vivo residence duration of human growth hormone loaded in nanogels comprising cinnamoyl alginate, cinnamoyl Pluronic F127 and cinnamoyl poly(ethylene glycol).
abstract: Cinnamoyl alginate, cinnamoyl Pluronic F127 and cinnamoyl poly(ethylene glycol) were self-assembled into nanogel in aqueous phase. The size of nanogel was 100nm-200nm on TEM photos. When 1ml of human growth hormone (hGH) solution (5mg/ml, pH3.0) was mixed with 4ml of the nanogel suspension (37.5mg/ml, pH3.0), 100% of hGH was loaded in the nanogels (corresponding to specific loading of 3.33% (w hGH/w nanogel)). In the in vivo pharmacokinetics study, the substantial blood level of hGH was maintained during the entire experiment period (two weeks) after one-time injection of hGH-loaded nanogel at 0.5mg hGH/kg body weight, and the area under curve (AUC) obtained with the one-time injection of hGH-loaded nanogel was higher than AUC obtained with daily injections of free hGH at 0.05mg hGH/kg for 7days. In the in vivo pharmacodynamics study, the blood level of insulin-like growth factor (IGF-1) increased in 2h and it was maintained during the entire experiment period (two weeks) after one-time injection of hGH-loaded nanogel at 0.5mg hGH/kg body weight. The in vivo fluorescence images of mice injected with rhodamine B isothiocyanate-labelled nanogel suspension showed fluorescence intensity up to 96h after intravenous injection, indicating that the in vivo residence time of the nanogel was greater than 96h.
authors.affiliation: Department of Medical Biomaterials Engineering, College of Biomedical Science and Institute of Bioscience and Biotechnology, Kangwon National University, 192-1, Hyoja 2 dong, Chuncheon, Kangwon-do 200-701, Republic of Korea.
authors.forename: Dong Youl
authors.initials: DY
authors.lastname: Yoon
authors.affiliation: Department of Medical Biomaterials Engineering, College of Biomedical Science and Institute of Bioscience and Biotechnology, Kangwon National University, 192-1, Hyoja 2 dong, Chuncheon, Kangwon-do 200-701, Republic of Korea. Electronic address: jinkim@kangwon.ac.kr.
authors.forename: Jin-Chul
authors.initials: JC
authors.lastname: Kim
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D002934:Cinnamates / Q000737:chemistry*; D005260:Female; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D019382:Human Growth Hormone / Q000097:blood / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D008812:Mice, Hairless; D000080385:Nanogels; D010316:Particle Size; D020442:Poloxamer / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012235:Rhodamines / Q000737:chemistry
chemical_list: D000464:Alginates; D002934:Cinnamates; D006603:Hexuronic Acids; D000080385:Nanogels; D012235:Rhodamines; C413692:polyethylene glycol polyethyleneimine nanogel; D020442:Poloxamer; D019382:Human Growth Hormone; D011092:Polyethylene Glycols; D007334:Insulin-Like Growth Factor I; D020723:Glucuronic Acid; D011094:Polyethyleneimine; C027755:rhodamine isothiocyanate
keywords: Human growth hormone; In vivo residence; Nanogel; Pharmacodynamics; Pharmacokinetics