title: Transcriptomic and proteomic analysis of mouse radiation-induced acute myeloid leukaemia (AML).
abstract: A combined transcriptome and proteome analysis of mouse radiation-induced AMLs using two primary AMLs, cell lines from these primaries, another cell line and its in vivo passage is reported. Compared to haematopoietic progenitor and stem cells (HPSC), over 5000 transcriptome alterations were identified, 2600 present in all materials. 55 and 3 alterations were detected in the proteomes of the cell lines and primary/in vivo passage material respectively, with one common to all materials. In cell lines, approximately 50% of the transcriptome changes are related to adaptation to cell culture, and in the proteome this proportion was higher. An AML 'signature' of 17 genes/proteins commonly deregulated in primary AMLs and cell lines compared to HPSCs was identified and validated using human AML transcriptome data. This also distinguishes primary AMLs from cell lines and includes proteins such as Coronin 1, pontin/RUVBL1 and Myeloperoxidase commonly implicated in human AML. C-Myc was identified as having a key role in radiation leukaemogenesis. These data identify novel candidates relevant to mouse radiation AML pathogenesis, and confirm that pathways of leukaemogenesis in the mouse and human share substantial commonality.
authors.affiliation: Radiation Effects Department, Centre for Radiation, Chemical and Environmental Hazards, Public Health England, Chilton, UK.
authors.forename: Christophe
authors.initials: C
authors.lastname: Badie
authors.affiliation: Faculty of Automatic Control, Electronics and Computer Science, Silesian University of Techology, Gliwice, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Blachowicz
authors.affiliation: Helmholtz Zentrum München, German Research Center for Environmental Health GmbH, Radiation Proteomics Group, Institute of Radiation Biology, Neuherberg, Germany.
authors.forename: Zarko
authors.initials: Z
authors.lastname: Barjaktarovic
authors.affiliation: Radiation Effects Department, Centre for Radiation, Chemical and Environmental Hazards, Public Health England, Chilton, UK.
authors.forename: Rosemary
authors.initials: R
authors.lastname: Finnon
authors.affiliation: Radiobiology Unit, Institute for Environment, Health and Safety, Belgian Nuclear Research Centre (SCK•.CEN), Mol, Belgium.
authors.forename: Arlette
authors.initials: A
authors.lastname: Michaux
authors.affiliation: Helmholtz Zentrum München, German Research Center for Environmental Health GmbH, Research Unit Protein Science, Neuherberg, Germany.
authors.forename: Hakan
authors.initials: H
authors.lastname: Sarioglu
authors.affiliation: Radiation Effects Department, Centre for Radiation, Chemical and Environmental Hazards, Public Health England, Chilton, UK.
authors.forename: Natalie
authors.initials: N
authors.lastname: Brown
authors.affiliation: Radiation Effects Department, Centre for Radiation, Chemical and Environmental Hazards, Public Health England, Chilton, UK.
authors.forename: Grainne
authors.initials: G
authors.lastname: Manning
authors.affiliation: Radiobiology Unit, Institute for Environment, Health and Safety, Belgian Nuclear Research Centre (SCK•.CEN), Mol, Belgium.
authors.forename: M Abderrafi
authors.initials: MA
authors.lastname: Benotmane
authors.affiliation: Helmholtz Zentrum München, German Research Center for Environmental Health GmbH, Radiation Proteomics Group, Institute of Radiation Biology, Neuherberg, Germany.
authors.forename: Soile
authors.initials: S
authors.lastname: Tapio
authors.affiliation: Faculty of Automatic Control, Electronics and Computer Science, Silesian University of Techology, Gliwice, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Polanska
authors.affiliation: Radiation Effects Department, Centre for Radiation, Chemical and Environmental Hazards, Public Health England, Chilton, UK.
authors.forename: Simon D
authors.initials: SD
authors.lastname: Bouffler
mesh_terms: D000074183:ATPases Associated with Diverse Cellular Activities / Q000378:metabolism; D000465:Algorithms; D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D045744:Cell Line, Tumor; D004265:DNA Helicases / Q000378:metabolism; D015973:Gene Expression Regulation, Leukemic; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008840:Microfilament Proteins / Q000378:metabolism; D009381:Neoplasms, Radiation-Induced / Q000378:metabolism* / Q000473:pathology; D009195:Peroxidase / Q000378:metabolism; D020543:Proteome; D015398:Signal Transduction; D059467:Transcriptome
chemical_list: D002352:Carrier Proteins; D008840:Microfilament Proteins; D020543:Proteome; C072061:coronin proteins; D009195:Peroxidase; C525849:TIP48 protein, mouse; D000074183:ATPases Associated with Diverse Cellular Activities; D004265:DNA Helicases; C486549:RUVBL1 protein, human
keywords: acute myeloid leukaemia; gene expression; ionising radiation; mouse; protein expression