title: A bimetallic nanocomposite modified genosensor for recognition and determination of thalassemia gene.
abstract: The main roles of DNA in the cells are to maintain and properly express genetic information. It is important to have analytical methods capable of fast and sensitive detection of DNA damage. DNA hybridization sensors are well suited for diagnostics and other purposes, including determination of bacteria and viruses. Beta thalassemias (βth) are due to mutations in the β-globin gene. In this study, an electrochemical biosensor which detects the sequences related to the β-globin gene issued from real samples amplified by polymerase chain reaction (PCR) is described for the first time. The biosensor relies on the immobilization of 20-mer single stranded oligonucleotide (probe) related to βth sequence on the carbon paste electrode (CPE) modified by 15% silver (Ag) and platinum (Pt) nanoparticles to prepare the bimetallic nanocomposite electrode and hybridization of this oligonucleotide with its complementary sequence (target). The extent of hybridization between the probe and target sequences was shown by using linear sweep voltammetry (LSV) with methylene blue (MB) as hybridization indicator. The selectivity of sensor was investigated using PCR samples containing non-complementary oligonucleotides. The detection limit of biosensor was calculated about 470.0pg/μL.
authors.affiliation: Eletroanalytical Chemistry Research Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran.
authors.forename: Ezat
authors.initials: E
authors.lastname: Hamidi-Asl
authors.affiliation: Eletroanalytical Chemistry Research Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran. Electronic address: j.raoof@umz.ac.ir.
authors.forename: Jahan Bakhsh
authors.initials: JB
authors.lastname: Raoof
authors.affiliation: Eletroanalytical Chemistry Research Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran.
authors.forename: Nahid
authors.initials: N
authors.lastname: Naghizadeh
authors.affiliation: Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran; Genetic Laboratory of Amirkola Children Hospital, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Haleh
authors.initials: H
authors.lastname: Akhavan-Niaki
authors.affiliation: Eletroanalytical Chemistry Research Laboratory, Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, Babolsar, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Ojani
authors.affiliation: Genetic Laboratory of Amirkola Children Hospital, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Banihashemi
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D006801:Humans; D008751:Methylene Blue / Q000737:chemistry; D009154:Mutation; D053761:Nanocomposites / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D013789:Thalassemia / Q000235:genetics*; D055544:beta-Globins / Q000235:genetics*
chemical_list: D009838:Oligodeoxyribonucleotides; D055544:beta-Globins; D008751:Methylene Blue
keywords: Bimetallic nanoparticles; Biosensor; Methylene blue; Polymerase chain reaction; β-Thalassemia