title: Development of Multifunctional Fluorescent-Magnetic Nanoprobes for Selective Capturing and Multicolor Imaging of Heterogeneous Circulating Tumor Cells.
abstract: Circulating tumor cells (CTC) are highly heterogeneous in nature due to epithelial-mesenchymal transition (EMT), which is the major obstacle for CTC analysis via "liquid biopsy". This article reports the development of a new class of multifunctional fluorescent-magnetic multicolor nanoprobes for targeted capturing and accurate identification of heterogeneous CTC. A facile design approach for the synthesis and characterization of bioconjugated multifunctonal nanoprobes that exhibit excellent magnetic properties and emit very bright and photostable multicolor fluorescence at red, green, and blue under 380 nm excitation is reported. Experimental data presented show that the multifunctional multicolor nanoprobes can be used for targeted capture and multicolor fluorescence mapping of heterogeneous CTC and can distinguish targeted CTC from nontargeted cells.
authors.affiliation: Department of Chemistry and Biochemistry, Jackson State University , Jackson, Mississippi 39217, United States.
authors.forename: Avijit
authors.initials: A
authors.lastname: Pramanik
authors.affiliation: Department of Chemistry and Biochemistry, Jackson State University , Jackson, Mississippi 39217, United States.
authors.forename: Aruna
authors.initials: A
authors.lastname: Vangara
authors.affiliation: Department of Chemistry and Biochemistry, Jackson State University , Jackson, Mississippi 39217, United States.
authors.forename: Bhanu Priya
authors.initials: BP
authors.lastname: Viraka Nellore
authors.affiliation: Department of Chemistry and Biochemistry, Jackson State University , Jackson, Mississippi 39217, United States.
authors.forename: Sudarson Sekhar
authors.initials: SS
authors.lastname: Sinha
authors.affiliation: Department of Chemistry and Biochemistry, Jackson State University , Jackson, Mississippi 39217, United States.
authors.forename: Suhash Reddy
authors.initials: SR
authors.lastname: Chavva
authors.affiliation: Department of Chemistry and Biochemistry, Jackson State University , Jackson, Mississippi 39217, United States.
authors.forename: Stacy
authors.initials: S
authors.lastname: Jones
authors.affiliation: Department of Chemistry and Biochemistry, Jackson State University , Jackson, Mississippi 39217, United States.
authors.forename: Paresh Chandra
authors.initials: PC
authors.lastname: Ray
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D002452:Cell Count; D045744:Cell Line, Tumor; D003952:Diagnostic Imaging / Q000295:instrumentation* / Q000379:methods*; D005456:Fluorescent Dyes / Q000378:metabolism*; D006801:Humans; D008280:Magnetics; D036103:Nanotechnology; D009360:Neoplastic Cells, Circulating / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D005456:Fluorescent Dyes
keywords: heterogeneous circulating tumor cell capturing; mapping of epithelial, mesenchymal, and stem cells simultaneously; multicolor fluorescence nanodots; multifunctional fluorescent−magnetic nanoprobes