title: Tegument Protein ORF45 Plays an Essential Role in Virion Morphogenesis of Murine Gammaherpesvirus 68.
abstract: Tegument proteins play critical roles in herpesvirus morphogenesis. ORF45 is a conserved tegument protein of gammaherpesviruses; however, its role in virion morphogenesis is largely unknown. In this work, we determined the ultrastructural localization of murine gammaherpesvirus 68 (MHV-68) ORF45 and found that this protein was incorporated into virions around the site of host-derived vesicles. Notably, the absence of ORF45 inhibited nucleocapsid egress and blocked cytoplasmic virion maturation, demonstrating that ORF45 is essential for MHV-68 virion morphogenesis.
authors.affiliation: Chinese Academy of Sciences Key Laboratory of Infection and Immunity, Institute of Biophysics, Beijing, People's Republic of China.|University of Chinese Academy of Sciences, Beijing, People's Republic of China.
authors.forename: Xing
authors.initials: X
authors.lastname: Jia
authors.affiliation: Chinese Academy of Sciences Key Laboratory of Infection and Immunity, Institute of Biophysics, Beijing, People's Republic of China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Shen
authors.affiliation: Chinese Academy of Sciences Key Laboratory of Infection and Immunity, Institute of Biophysics, Beijing, People's Republic of China.|University of Chinese Academy of Sciences, Beijing, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Lv
authors.affiliation: Chinese Academy of Sciences Key Laboratory of Infection and Immunity, Institute of Biophysics, Beijing, People's Republic of China.|University of Chinese Academy of Sciences, Beijing, People's Republic of China.
authors.forename: Ziwei
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Chinese Academy of Sciences Key Laboratory of Infection and Immunity, Institute of Biophysics, Beijing, People's Republic of China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Guo
authors.affiliation: Chinese Academy of Sciences Key Laboratory of Infection and Immunity, Institute of Biophysics, Beijing, People's Republic of China hydeng@moon.ibp.ac.cn.|CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, People's Republic of China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Deng
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D004847:Epithelial Cells; D017874:Immediate-Early Proteins / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D009024:Morphogenesis; D018144:Rhadinovirus / Q000502:physiology*; D014771:Virion / Q000378:metabolism*; D019065:Virus Assembly; D057074:Virus Release
chemical_list: D017874:Immediate-Early Proteins; C500426:ORF45 protein, murine gammaherpesvirus 68