title: Assessment of incretins in oral glucose and lipid tolerance tests may be indicative in the diagnosis of metabolic syndrome aggravation.
abstract: Incretins stimulated by oral meals are claimed to be protective for the pancreatic beta cells, to increase insulin secretion, to inhibit glucagon release, slow gastric emptying (glucagon-like peptide-1) and suppress appetite. Recently it has however been suggested that glucagon-like peptide-1 (GLP-1) is putative early biomarker of metabolic consequences of the obesity associated proinflammatory state. The study was aimed to compare the release of incretins and some of early markers of inflammation at the fasting and postprandial period induced by functional oral glucose as well as lipid load in healthy controls and patients with metabolic syndrome (MS) to see if functional tests may be helpful in searching for the inflammatory status of patients. Fifty patients with MS and 20 healthy volunteers (C) participated in this study. The 3-hour oral glucose (OGTT) and the 8-hour oral lipid (OLTT) tolerance tests were performed. At fasting leptin and adiponectin, as well as every 30 minutes of OGTT and every 2 hours of OLTT blood concentration of GLP-1, glucose-dependent insulinotropic polypeptide (GIP), glucose, insulin, triglycerides, free fatty acids, glutathione peroxidase, interleukin-6, sE-selectin, monocyte chemoattractant protein-1 (MCP1) and visfatin were measured. At fasting and during both OGTT and OLTT the level of incretins did not differ between the MS and the C group. Both glucose and lipids reach food activated incretins secretion. Glucose was the main GLP-1 release activator, while the lipid load activated evidently GIP secretion. A significantly larger AUC-GIP after the lipid-rich meal over the carbohydrate meal was observed, while statistically bigger value of AUC-GLP-1 was noticed in OGTT than in OLTT (P < 0.001) within each of the investigated groups. In patients with the highest fasting plasma GIP concentration (3(rd) tertile), IL-6, MCP-1, sE-selectin and visfatin blood levels were increased and correlated with glutathione peroxydase, leptin/adiponectin ratio, higher visfatin and interleukin-6 levels. The fat containing meals stimulate the long-lasting release of incretins, mainly GIP, parallel to the increase of the markers of low grade inflammation associating obesity in metabolic syndrome. The possibility of use of the postprandial (OLTT) GIP release measurement for the low grade inflammation progress in MS patients is suggested.
authors.affiliation: Chair and Department of Endocrinology, Jagiellonian University, Collegium Medicum, Cracow, Poland. m.kiec-klimczak@cm-uj.krakow.pl.
authors.forename: M
authors.initials: M
authors.lastname: Kiec-Klimczak
authors.affiliation: Chair and Department of Clinical Biochemistry, Jagiellonian University, Collegium Medicum, Cracow, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Malczewska-Malec
authors.affiliation: Chair and Department of Clinical Biochemistry, Jagiellonian University, Collegium Medicum, Cracow, Poland.
authors.forename: U
authors.initials: U
authors.lastname: Razny
authors.affiliation: Chair and Department of Clinical Biochemistry, Jagiellonian University, Collegium Medicum, Cracow, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Zdzienicka
authors.affiliation: Chair and Department of Clinical Biochemistry, Jagiellonian University, Collegium Medicum, Cracow, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Gruca
authors.affiliation: Chair and Department of Clinical Biochemistry, Jagiellonian University, Collegium Medicum, Cracow, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Goralska
authors.affiliation: Chair and Department of Endocrinology, Jagiellonian University, Collegium Medicum, Cracow, Poland.
authors.forename: D
authors.initials: D
authors.lastname: Pach
authors.affiliation: Chair and Department of Endocrinology, Jagiellonian University, Collegium Medicum, Cracow, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Gilis-Januszewska
authors.affiliation: Chair and Department of Clinical Biochemistry, Jagiellonian University, Collegium Medicum, Cracow, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Dembinska-Kiec
authors.affiliation: Chair and Department of Endocrinology, Jagiellonian University, Collegium Medicum, Cracow, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Hubalewska-Dydejczyk
mesh_terms: D052242:Adiponectin / Q000097:blood; D000328:Adult; D000368:Aged; D001786:Blood Glucose / Q000032:analysis; D016207:Cytokines / Q000097:blood; D019040:E-Selectin / Q000097:blood; D005215:Fasting / Q000097:blood*; D005260:Female; D005749:Gastric Inhibitory Polypeptide / Q000097:blood*; D052216:Glucagon-Like Peptide 1 / Q000097:blood; D005951:Glucose Tolerance Test; D006801:Humans; D007328:Insulin / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D020738:Leptin / Q000097:blood; D008055:Lipids / Q000097:blood; D008297:Male; D024821:Metabolic Syndrome / Q000097:blood* / Q000175:diagnosis; D008875:Middle Aged; D054409:Nicotinamide Phosphoribosyltransferase / Q000097:blood; D019518:Postprandial Period / Q000502:physiology*
chemical_list: D052242:Adiponectin; D001786:Blood Glucose; D016207:Cytokines; D019040:E-Selectin; C508600:IL6 protein, human; D007328:Insulin; D015850:Interleukin-6; D020738:Leptin; D008055:Lipids; C546936:SELE protein, human; D005749:Gastric Inhibitory Polypeptide; D052216:Glucagon-Like Peptide 1; D054409:Nicotinamide Phosphoribosyltransferase; C085455:nicotinamide phosphoribosyltransferase, human