title: The dynamic transcriptional and translational landscape of the model antibiotic producer Streptomyces coelicolor A3(2).
abstract: Individual Streptomyces species have the genetic potential to produce a diverse array of natural products of commercial, medical and veterinary interest. However, these products are often not detectable under laboratory culture conditions. To harness their full biosynthetic potential, it is important to develop a detailed understanding of the regulatory networks that orchestrate their metabolism. Here we integrate nucleotide resolution genome-scale measurements of the transcriptome and translatome of Streptomyces coelicolor, the model antibiotic-producing actinomycete. Our systematic study determines 3,570 transcription start sites and identifies 230 small RNAs and a considerable proportion (∼21%) of leaderless mRNAs; this enables deduction of genome-wide promoter architecture. Ribosome profiling reveals that the translation efficiency of secondary metabolic genes is negatively correlated with transcription and that several key antibiotic regulatory genes are translationally induced at transition growth phase. These findings might facilitate the design of new approaches to antibiotic discovery and development.
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea.|KAIST Institute for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea.
authors.forename: Yujin
authors.initials: Y
authors.lastname: Jeong
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Korea.
authors.forename: Ji-Nu
authors.initials: JN
authors.lastname: Kim
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Korea.
authors.forename: Min Woo
authors.initials: MW
authors.lastname: Kim
authors.affiliation: Department of Microbial Sciences, Faculty of Health and Medical Sciences, University of Surrey, Surrey GU2 7XH, UK.
authors.forename: Giselda
authors.identifier: 0000-0001-5286-7771
authors.initials: G
authors.lastname: Bucca
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea.|KAIST Institute for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea.
authors.forename: Suhyung
authors.initials: S
authors.lastname: Cho
authors.affiliation: Department of Chemistry and Nano Science, Ewha Womans University, Seoul 120-750, Korea.
authors.forename: Yeo Joon
authors.initials: YJ
authors.lastname: Yoon
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Korea.
authors.forename: Byung-Gee
authors.initials: BG
authors.lastname: Kim
authors.affiliation: School of Biological Sciences, Seoul National University, Seoul 151-742, Korea.
authors.forename: Jung-Hye
authors.initials: JH
authors.lastname: Roe
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea.|KAIST Institute for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea.|Intelligent Synthetic Biology Center, Daejeon 305-701, Korea.
authors.forename: Sun Chang
authors.initials: SC
authors.lastname: Kim
authors.affiliation: Department of Microbial Sciences, Faculty of Health and Medical Sciences, University of Surrey, Surrey GU2 7XH, UK.
authors.forename: Colin P
authors.initials: CP
authors.lastname: Smith
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea.|KAIST Institute for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea.|Intelligent Synthetic Biology Center, Daejeon 305-701, Korea.
authors.forename: Byung-Kwan
authors.identifier: 0000-0003-4788-4184
authors.initials: BK
authors.lastname: Cho
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry; D015964:Gene Expression Regulation, Bacterial; D063990:Gene Ontology; D053263:Gene Regulatory Networks; D016680:Genome, Bacterial; D058977:Molecular Sequence Annotation; D015394:Molecular Structure; D014176:Protein Biosynthesis; D058727:RNA, Small Untranslated / Q000235:genetics / Q000378:metabolism; D012270:Ribosomes / Q000235:genetics / Q000378:metabolism; D064210:Secondary Metabolism / Q000235:genetics; D048372:Streptomyces coelicolor / Q000235:genetics* / Q000378:metabolism; D024363:Transcription Initiation Site; D014158:Transcription, Genetic
chemical_list: D000900:Anti-Bacterial Agents; D058727:RNA, Small Untranslated