title: MLX Is a Transcriptional Repressor of the Mammalian Golgi Stress Response.
abstract: The Golgi stress response is a homeostatic mechanism that controls the capacity of the Golgi apparatus in accordance with cellular demands. When the capacity of the Golgi apparatus becomes insufficient (Golgi stress), transcription levels of Golgi-related genes encoding glycosylation enzymes, a Golgi structural protein, and components of vesicular transport are upregulated through a common cis-acting enhancer-the Golgi apparatus stress response element (GASE). Here, we identified the transcription factor MLX as a GASE-binding protein. MLX resides in the cytoplasm and does not bind to GASE in normal growth conditions, whereas MLX translocates into the nucleus and specifically binds to GASE in response to Golgi stress. Suppression of MLX expression increased transcriptional induction of target genes of the Golgi stress response, whereas overexpression of MLX reduced GASE-binding of TFE3 as well as transcriptional induction from GASE, suggesting that MLX is a transcriptional repressor of the mammalian Golgi stress response.
authors.affiliation: Department of Molecular Biochemistry, Graduate School of Life Science, University of Hyogo.
authors.forename: Mai
authors.initials: M
authors.lastname: Taniguchi
authors.forename: Kanae
authors.initials: K
authors.lastname: Sasaki-Osugi
authors.forename: Masaya
authors.initials: M
authors.lastname: Oku
authors.forename: Shogo
authors.initials: S
authors.lastname: Sawaguchi
authors.forename: Soichiro
authors.initials: S
authors.lastname: Tanakura
authors.forename: Yumeto
authors.initials: Y
authors.lastname: Kawai
authors.forename: Sadao
authors.initials: S
authors.lastname: Wakabayashi
authors.forename: Hiderou
authors.initials: H
authors.lastname: Yoshida
mesh_terms: D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D015152:Blotting, Northern; D047369:Chromatin Immunoprecipitation; D005786:Gene Expression Regulation / Q000235:genetics; D017930:Genes, Reporter / Q000235:genetics; D006056:Golgi Apparatus / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D008856:Microscopy, Fluorescence; D011485:Protein Binding; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D020218:Response Elements / Q000235:genetics
chemical_list: D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; C403128:MLX protein, human; D012333:RNA, Messenger; D034741:RNA, Small Interfering