title: Effects of platelet-rich plasma on liver regeneration in CCl4-induced hepatotoxicity model.
abstract: Numerous bioactive growth factors and cytokines in platelet-rich plasma (PRP) have recently made it an attractive biomaterial for therapeutic purposes. These growth factors have the potential to regenerate the injured tissues. The aim of this study was to investigate the therapeutic effects of PRP in hepatotoxic animal model. Hepatotoxicity was induced in rats by oral administration of 4 mL/kg/week of CCl4diluted 1:1 in corn oil for 10 weeks. To confirm the hepatotoxicity, 24 h after the last CCl4administration, blood samples were collected via cardiac puncture to assess the serum levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, albumin, total protein, and total bilirubin. Twenty-four hours after blood collection, the experimental animals received a single injection of PRP (1 mL) via the anterior mesenteric vein. One week later, all biochemical tests were performed again, and the rats were scarified and their livers were removed, prepared histologically, and stained. The stereological analyses were performed to evaluate the effects of PRP on histopathological features of CCl4-treated livers. The results were compared statistically with the corresponding control and CCl4+normal saline (NS)-treated animals. A significant decrease in the number and volume of hepatocytes (p = 0.01), and also a reduction in the volume of sinusoids (p = 0.001) and connective tissue (p = 0.04), were observed in the PRP-treated animals compared with the CCl4+NS-treated ones. Our findings demonstrated that application of PRP had beneficial effects on CCl4-induced fibrosis; however, it had detrimental effects on the total number of hepatocytes and the volume of hepatocytes and sinusoidal spaces.
authors.affiliation: a Anatomy Department , Shiraz University of Medical Sciences , Shiraz , Iran.
authors.forename: Afsaneh
authors.initials: A
authors.lastname: Mafi
authors.affiliation: b Anatomy Department , Stereology and Histomorphology Research Center, Shiraz University of Medical Sciences , Shiraz , Iran.
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Dehghani
authors.affiliation: a Anatomy Department , Shiraz University of Medical Sciences , Shiraz , Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Moghadam
authors.affiliation: b Anatomy Department , Stereology and Histomorphology Research Center, Shiraz University of Medical Sciences , Shiraz , Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Noorafshan
authors.affiliation: c Anatomy Department , Laboratory for Stem Cell Research, Shiraz University of Medical Sciences , Shiraz , Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Vojdani
authors.affiliation: d Anatomy Department , Tissue Engineering Lab, Shiraz University of Medical Sciences , Shiraz , Iran.
authors.forename: Tahereh
authors.initials: T
authors.lastname: Talaei-Khozani
mesh_terms: D000818:Animals; D002251:Carbon Tetrachloride / Q000633:toxicity; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood / Q000175:diagnosis / Q000628:therapy*; D003238:Connective Tissue / Q000187:drug effects / Q000473:pathology; D004195:Disease Models, Animal; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008111:Liver Function Tests; D008115:Liver Regeneration; D008297:Male; D009929:Organ Size; D010976:Platelet Count; D053657:Platelet-Rich Plasma; D051381:Rats; D016896:Treatment Outcome
chemical_list: D002251:Carbon Tetrachloride
keywords: Growth factors; liver; platelet-rich plasma; regeneration