title: The microscale evolution of the erosion front of blood clots exposed to ultrasound stimulated microbubbles.
abstract: Serial two-photon microscopy of blood clots with fluorescently tagged fibrin networks was conducted during microbubble-mediated sonothrombolysis to examine the microscale evolution of the resulting erosion front. The development of a complex zonal erosion pattern was observed, comprised of a cell depleted layer of fibrin network overlying intact clot which then underwent progressive recession. The fibrin zone architecture was dependent on exposure conditions with 0.1 MPa causing no erosion, 0.39 MPa resulting in homogenous structure, and combination 0.39/0.96 MPa pulses forming large-scale tunnels. High speed imaging and Coulter counter data indicated the fibrin zone formation process involves the ejection of intact erythrocytes.
authors.affiliation: Department of Medical Biophysics, University of Toronto, Toronto M5S 1A1, Canada chrisacc@sri.utoronto.ca.
authors.forename: Christopher N
authors.initials: CN
authors.lastname: Acconcia
authors.affiliation: Sunnybrook Research Institute, 2075 Bayview Avenue, M4N 3M5 Toronto, Canada bleung@sri.utoronto.ca.
authors.forename: Ben Y C
authors.initials: BY
authors.lastname: Leung
authors.affiliation: Department of Medical Biophysics, University of Toronto, Toronto M5S 1A1, Canada goertz@sri.utoronto.ca.
authors.forename: David E
authors.initials: DE
authors.lastname: Goertz
mesh_terms: D004912:Erythrocytes / Q000378:metabolism*; D005337:Fibrin / Q000378:metabolism*; D005342:Fibrinolysis; D006801:Humans; D061185:Mechanical Thrombolysis / Q000379:methods*; D045423:Microbubbles; D036641:Microscopy, Fluorescence, Multiphoton; D013927:Thrombosis / Q000097:blood / Q000628:therapy*; D013997:Time Factors; D014464:Ultrasonic Therapy / Q000379:methods*
chemical_list: D005337:Fibrin