title: Cell optoporation with a sub-15 fs and a 250-fs laser.
abstract: We employed two commercially available femtosecond lasers, a Ti:sapphire and a ytterbium-based oscillator, to directly compare from a user’s practical point-of-view in one common experimental setup the efficiencies of transient laser-induced cell membrane permeabilization, i.e., of so-called optoporation. The experimental setup consisted of a modified multiphoton laser-scanning microscope employing high-NA focusing optics. An automatic cell irradiation procedure was realized with custom-made software that identified cell positions and controlled relevant hardware components. The Ti:sapphire and ytterbium-based oscillators generated broadband sub-15-fs pulses around 800 nm and 250-fs pulses at 1044 nm, respectively. A higher optoporation rate and posttreatment viability were observed for the shorter fs pulses, confirming the importance of multiphoton effects for efficient optoporation.
authors.affiliation: JenLab GmbH, Science Park 2, Campus D 1.2, 66123 Saarbrücken, Germany and Schillerstr. 1, 07745 Jena, Germany.
authors.forename: Hans Georg
authors.initials: HG
authors.lastname: Breunig
authors.affiliation: JenLab GmbH, Science Park 2, Campus D 1.2, 66123 Saarbrücken, Germany and Schillerstr. 1, 07745 Jena, GermanybSaarland University, Department of Biophotonics and Laser Technology, Campus A5.1, 66123 Saarbrücken, Germany.
authors.forename: Ana
authors.initials: A
authors.lastname: Batista
authors.affiliation: JenLab GmbH, Science Park 2, Campus D 1.2, 66123 Saarbrücken, Germany and Schillerstr. 1, 07745 Jena, GermanybSaarland University, Department of Biophotonics and Laser Technology, Campus A5.1, 66123 Saarbrücken, Germany.
authors.forename: Aisada
authors.initials: A
authors.lastname: Uchugonova
authors.affiliation: JenLab GmbH, Science Park 2, Campus D 1.2, 66123 Saarbrücken, Germany and Schillerstr. 1, 07745 Jena, GermanybSaarland University, Department of Biophotonics and Laser Technology, Campus A5.1, 66123 Saarbrücken, Germany.
authors.forename: Karsten
authors.initials: K
authors.lastname: König
mesh_terms: D002462:Cell Membrane / Q000737:chemistry / Q000528:radiation effects*; D003584:Cytological Techniques / Q000295:instrumentation* / Q000379:methods*; D007834:Lasers; D018613:Microscopy, Confocal; D055095:Optics and Photonics; D014025:Titanium; D015018:Ytterbium
chemical_list: D014025:Titanium; D015018:Ytterbium