title: [Predictive and prognostic factors of gastric cancer].
abstract: BACKGROUND
  Gastric cancer is a frequent malignant disease with poor prognosis. Most patients undergo only palliative treatment. Chemotherapy is another alternative but its effect differs in individual patients.
  
  
  METHODS
  This is retrospective study. We enrolled 54 patients (N=54) according to the inclusion criteria. We performed quantification of gene expression of selected genes and some microRNA from tumour tissue, which was used for the diagnosis. Statistical analysis of the data was performed.
  
  
  RESULTS
  We demonstrated a predictive value of gene expression of thynidylate synthase in tumour tissue for a therapeutic effect of chemotherapy based on 5-Fluorouracil or Capecitabine. At the same time, we demonstrated a predictive value of miR181, miR150, mir192 and miR342 microRNA levels from the tumour tissue. In addition, we succeeded to demonstrate a predictive value of miR221, miR224, miR520 and miR375 microRNA levels for a therapeutic effect of chemotherapy based on platinum derivates.
  
  
  CONCLUSIONS
  Thanks to the use of efficient therapy predictors, we can distinguish those patients who will profit from chemotherapy from patients where an effect cannot be expected. Thanks to personified oncology therapy the quality of life of some patients can be improved while reducing the costs of the therapy by avoiding inefficient chemotherapy. Only an early diagnosis of gastric cancer can reverse the adverse prognosis of patients with this disease.
  
  
  BACKGROUND
  gastric cancer - microRNA - prognostic markers - predictive markers.
authors.forename: D
authors.initials: D
authors.lastname: Šmíd
authors.forename: D
authors.initials: D
authors.lastname: Kubačková
authors.forename: J
authors.initials: J
authors.lastname: Doležal
authors.forename: J
authors.initials: J
authors.lastname: Fichtl
authors.forename: M
authors.initials: M
authors.lastname: Pešta
authors.forename: V
authors.initials: V
authors.lastname: Kulda
authors.forename: O
authors.initials: O
authors.lastname: Daum
authors.forename: V
authors.initials: V
authors.lastname: Třeška
authors.forename: T
authors.initials: T
authors.lastname: Skalický
mesh_terms: D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D000069287:Capecitabine / Q000008:administration & dosage; D005472:Fluorouracil / Q000008:administration & dosage; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D011379:Prognosis; D011788:Quality of Life; D012189:Retrospective Studies; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000235:genetics*; D013940:Thymidylate Synthase / Q000235:genetics; D059467:Transcriptome; D016896:Treatment Outcome
chemical_list: D035683:MicroRNAs; D000069287:Capecitabine; D013940:Thymidylate Synthase; D005472:Fluorouracil