title: Extraction, purification and anti-proliferative activities of polysaccharides from Lentinus edodes.
abstract: In this study, the enzyme-assisted extraction of polysaccharides from Lentinus edodes (LEPs) was optimized by response surface methodology, and a preliminary characterization of the extracted LEPs and their anti-proliferative activities were investigated. An orthogonal assay was constructed to determine the optimal amounts of cellulase, papain and pectinase, which were 15, 20 and 15g/kg, respectively. Then effects of extraction conditions were evaluated and optimized using a Box-Behnken design. The results showed that the highest polysaccharides yield of 15.65% was achieved with an extraction temperature of 54°C, pH 5.0, enzymatic treatment time of 93min and a liquid/material ratio of 29:1mL/g, which correlated well with the predicted yield of 15.58%. Subsequently, the crude LEPs were further purified by DEAE-cellulose and Sephadex-100 chromatography to obtain two fractions, which were designated as LEP-1 and LEP-2 and their monosaccharide compositions were characterized by GC. Fourier-transform infrared spectra demonstrated that LEP-1 and LEP-2 were distinct from each other regarding their chemical structures. In addition, the LEPs exhibited inhibition of cell proliferation on HCT-116 and HeLa cells in vitro. In summary, this study provides an efficient enzyme-assisted extraction for LEPs, which can be used as natural antitumor agents in the pharmaceutical and functional food industries.
authors.affiliation: Department of Pharmacy, Hebei North University, Zhangjiakou 075000, PR China.
authors.forename: Yong-Ming
authors.initials: YM
authors.lastname: Zhao
authors.affiliation: Department of Pharmacy, Hebei North University, Zhangjiakou 075000, PR China; Hebei Medical University, Shijiazhuang 050017, PR China.
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, Hebei North University, Zhangjiakou 075000, PR China; Hebei Medical University, Shijiazhuang 050017, PR China.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Wu
authors.affiliation: Department of Pharmacy, Hebei North University, Zhangjiakou 075000, PR China.
authors.forename: Jian-Ming
authors.initials: JM
authors.lastname: Yang
authors.affiliation: Department of Pharmacy, Hebei North University, Zhangjiakou 075000, PR China. Electronic address: leewei318@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Pharmacy, Hebei North University, Zhangjiakou 075000, PR China. Electronic address: shenlixiacom@163.com.
authors.forename: Li-Xia
authors.initials: LX
authors.lastname: Shen
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects*; D002480:Cellulase / Q000737:chemistry; D004354:Drug Screening Assays, Antitumor; D062610:Fungal Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology; D045325:HCT116 Cells; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D059625:Liquid-Liquid Extraction; D051379:Mice; D009368:Neoplasm Transplantation; D010206:Papain / Q000737:chemistry; D011096:Polygalacturonase / Q000737:chemistry; D012509:Sarcoma / Q000188:drug therapy; D020942:Shiitake Mushrooms / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D062610:Fungal Polysaccharides; D011096:Polygalacturonase; D002480:Cellulase; D010206:Papain
keywords: Anti- proliferative activity; Enzyme-assisted extraction; Lentinus edodes polysaccharides