title: Molecularly Imprinted Polymer Coated Quantum Dots for Multiplexed Cell Targeting and Imaging.
abstract: Advanced tools for cell imaging are of great interest for the detection, localization, and quantification of molecular biomarkers of cancer or infection. We describe a novel photopolymerization method to coat quantum dots (QDs) with polymer shells, in particular, molecularly imprinted polymers (MIPs), by using the visible light emitted from QDs excited by UV light. Fluorescent core-shell particles specifically recognizing glucuronic acid (GlcA) or N-acetylneuraminic acid (NANA) were prepared. Simultaneous multiplexed labeling of human keratinocytes with green QDs conjugated with MIP-GlcA and red QDs conjugated with MIP-NANA was demonstrated by fluorescence imaging. The specificity of binding was verified with a non-imprinted control polymer and by enzymatic cleavage of the terminal GlcA and NANA moieties. The coating strategy is potentially a generic method for the functionalization of QDs to address a much wider range of biocompatibility and biorecognition issues.
authors.affiliation: Sorbonne Universités, Université de Technologie de Compiègne, CNRS Enzyme and Cell Engineering Laboratory, Rue Roger Couttolenc, CS 60319, 60203, Compiègne Cedex, France.
authors.forename: Maria
authors.initials: M
authors.lastname: Panagiotopoulou
authors.affiliation: School of Biological and Chemical Sciences, Queen Mary University of London, London, E1 4NS, UK.
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Salinas
authors.affiliation: Sorbonne Universités, Université de Technologie de Compiègne, CNRS Enzyme and Cell Engineering Laboratory, Rue Roger Couttolenc, CS 60319, 60203, Compiègne Cedex, France.
authors.forename: Selim
authors.initials: S
authors.lastname: Beyazit
authors.affiliation: Sorbonne Universités, Université de Technologie de Compiègne, CNRS Enzyme and Cell Engineering Laboratory, Rue Roger Couttolenc, CS 60319, 60203, Compiègne Cedex, France.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Kunath
authors.affiliation: Sorbonne Universités, Université de Technologie de Compiègne, CNRS Enzyme and Cell Engineering Laboratory, Rue Roger Couttolenc, CS 60319, 60203, Compiègne Cedex, France.
authors.forename: Luminita
authors.initials: L
authors.lastname: Duma
authors.affiliation: Sorbonne Universités, Université de Technologie de Compiègne, CNRS Enzyme and Cell Engineering Laboratory, Rue Roger Couttolenc, CS 60319, 60203, Compiègne Cedex, France.
authors.forename: Elise
authors.initials: E
authors.lastname: Prost
authors.affiliation: School of Chemistry, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK.
authors.forename: Andrew G
authors.initials: AG
authors.lastname: Mayes
authors.affiliation: School of Biological and Chemical Sciences, Queen Mary University of London, London, E1 4NS, UK.
authors.forename: Marina
authors.initials: M
authors.lastname: Resmini
authors.affiliation: Sorbonne Universités, Université de Technologie de Compiègne, CNRS Enzyme and Cell Engineering Laboratory, Rue Roger Couttolenc, CS 60319, 60203, Compiègne Cedex, France. jeanne.tse-sum-bui@utc.fr.
authors.forename: Bernadette
authors.initials: B
authors.lastname: Tse Sum Bui
authors.affiliation: Sorbonne Universités, Université de Technologie de Compiègne, CNRS Enzyme and Cell Engineering Laboratory, Rue Roger Couttolenc, CS 60319, 60203, Compiègne Cedex, France. karsten.haupt@utc.fr.
authors.forename: Karsten
authors.initials: K
authors.lastname: Haupt
mesh_terms: D006801:Humans; D015603:Keratinocytes / Q000166:cytology*; D054802:Molecular Imprinting; D061848:Optical Imaging; D011108:Polymers / Q000737:chemistry*; D045663:Quantum Dots / Q000737:chemistry*
chemical_list: D011108:Polymers
keywords: bioimaging; molecularly imprinted polymers; nanocomposites; quantum dots; synthetic receptors