title: Identification of Factors Produced and Secreted by Mesenchymal Stromal Cells with the SILAC Method.
abstract: Mesenchymal stromal cells (MSCs) secrete a large variety of proteins and factors, which shape the secretome. These proteins participate in multiple cellular functions, including the promotion of regenerative processes in the damaged tissue. Secretomes derived from either undifferentiated MSCs or these cells undergoing osteogenic, chondrogenic, or adipogenic differentiation have been characterized using different liquid chromatography tandem mass spectrometry (LC-MS/MS)-based quantitative proteomic approaches. In this chapter, we describe the use of the Stable Isotope Labeling by Amino Acids in Cell culture (SILAC) strategy for the identification and relative quantification of the mesenchymal stromal cell secretome, specifically during chondrogenesis.
authors.affiliation: Rheumatology Division, ProteoRed-PRB2/ISCIII Proteomics Group, INIBIC - Hospital Universitario de A Coruña, A Coruña, 15006, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Rocha
authors.affiliation: Rheumatology Division, ProteoRed-PRB2/ISCIII Proteomics Group, INIBIC - Hospital Universitario de A Coruña, A Coruña, 15006, Spain.
authors.forename: Valentina
authors.initials: V
authors.lastname: Calamia
authors.affiliation: Rheumatology Division, ProteoRed-PRB2/ISCIII Proteomics Group, INIBIC - Hospital Universitario de A Coruña, A Coruña, 15006, Spain.|RIER-RED de Inflamación y Enfermedades Reumáticas, INIBIC-CHUAC, A Coruña, Spain.|Servicio de Reumatología, Unidad de Investigación, INIBIC-CHU A Coruña, C/Xubias, 84, A Coruña, 15006, Spain.
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Blanco
authors.affiliation: Rheumatology Division, ProteoRed-PRB2/ISCIII Proteomics Group, INIBIC - Hospital Universitario de A Coruña, A Coruña, 15006, Spain. cristina.ruiz.romero@sergas.es.|CIBER-BBN, INIBIC-CHUAC, A Coruña, Spain. cristina.ruiz.romero@sergas.es.
authors.forename: Cristina
authors.initials: C
authors.lastname: Ruiz-Romero
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002478:Cells, Cultured; D020219:Chondrogenesis; D002853:Chromatography, Liquid; D017077:Culture Media, Conditioned / Q000302:isolation & purification; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D017077:Culture Media, Conditioned; D020543:Proteome
keywords: Chondrogenesis; Conditioned medium; Mass spectrometry; Mesenchymal stromal cells; Proteomics; SILAC; Secretome