title: Detection of biofilm related genes, classical enterotoxin genes and agr typing among Staphylococcus aureus isolated from bovine with subclinical mastitis in southwest of Iran.
abstract: Staphylococcus aureus by producing biofilm and facilitating chronic infection is a common cause of mastitis in cows and thereby can cause food poisoning by production of enterotoxins in milk. The agr typing method is an important tool for epidemiological investigation about S. aureus. The aims of the present study were to detect biofilm related genes, 5 classical enterotoxin genes and the agr types among S. aureus isolates. The ability of S. aureus isolates to produce biofilm was evaluated by modified CRA plate. Six biofilm related adhesion genes (icaD, icaA, fnbA, bap, clfA and cna), five classical enterotoxin genes (sea, seb, sec, sed and see) and tst-1 gene were detected by PCR methods. Multiplex-PCR was used to determination of the agr groups. 55 out of 80(68.8%) S. aureus isolates were biofilm producer. The icaD gene was detected in 70 (87.5%) of isolates. The prevalence rates of fnbA, icaA, clfA, cna and bap were 72.5, 56.25, 50, 22.5, and 5% respectively. The agr group I and III were detected in 57.5% 25% of studied isolates. The sea, sed and tst-1 genes were found in 10%, 7.5% and 1.25% of isolates respectively. The majority of S. aureus were able to produce biofilm. Significant associations were observed between presence of the icaD, icaA, fnbA, clfA and the cna genes as well as biofilm formation. The present study revealed that isolates with the agr type III are more potent for biofilm production. Our data supported a possible link between the agr types and certain SE genes.
authors.affiliation: Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran. Electronic address: khoramrooz@gmail.com.
authors.forename: Seyed Sajjad
authors.initials: SS
authors.lastname: Khoramrooz
authors.affiliation: Department of Basic Sciences, Islamic Azad University, Yasooj Branch, Yasooj, Iran.
authors.forename: Fariba
authors.initials: F
authors.lastname: Mansouri
authors.affiliation: Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran.
authors.forename: Masoud
authors.initials: M
authors.lastname: Marashifard
authors.affiliation: Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran.
authors.forename: Seyed Ali Asghar
authors.initials: SA
authors.lastname: Malek Hosseini
authors.affiliation: Department of Basic Sciences, Islamic Azad University, Yasooj Branch, Yasooj, Iran.
authors.forename: Fereshteh
authors.initials: F
authors.lastname: Akbarian Chenarestane-Olia
authors.affiliation: Department of Basic Sciences, Islamic Azad University, Yasooj Branch, Yasooj, Iran.
authors.forename: Banafsheh
authors.initials: B
authors.lastname: Ganavehei
authors.affiliation: School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Gharibpour
authors.affiliation: Department of Surgery, Yasuj University of Medical Sciences, Yasuj, Iran.
authors.forename: Ardavan
authors.initials: A
authors.lastname: Shahbazi
authors.affiliation: School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Mirzaii
authors.affiliation: Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Davood
authors.initials: D
authors.lastname: Darban-Sarokhalil
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D018441:Biofilms / Q000254:growth & development*; D002417:Cattle; D004768:Enterotoxins / Q000235:genetics*; D005838:Genotype; D007492:Iran / Q000453:epidemiology; D008414:Mastitis, Bovine / Q000453:epidemiology / Q000382:microbiology*; D017720:Molecular Epidemiology; D058889:Molecular Typing; D060885:Multiplex Polymerase Chain Reaction; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D015534:Trans-Activators / Q000235:genetics*
chemical_list: D018829:Adhesins, Bacterial; C095698:Agr protein, Staphylococcus aureus; D001426:Bacterial Proteins; D004768:Enterotoxins; D015534:Trans-Activators
keywords: Biofilm; Enterotoxin genes; Mastitis; Staphylococcus aureus; agr typing