title: In vitro hemocompatibility and cytocompatibility of a three-layered vascular scaffold fabricated by sequential electrospinning of PCL, collagen, and PLLA nanofibers.
abstract: Aiming to mimic a blood vessel structurally, morphologically, and mechanically, a sequential electrospinning technique using a small diameter mandrel collector was performed and a three-layered tubular scaffold composed of nanofibers of polycaprolactone, collagen, and poly(l-lactic acid) as inner, intermediate, and outer layers, respectively, was developed. Biological performances of the scaffold in terms of compatibility with blood and endothelial cells were assessed to get some insights into its potential use as a tissue engineered small-diameter vascular replacement compared to an expanded polytetrafluoroethylene vascular graft. Due to direct contact of the blood and endothelial cells with inner surface of the scaffold, polycaprolactone fibers were characterized using SEM, water contact angle measurement, and ATR-FTIR. Despite similar surface wettability of the electrospun scaffold and the expanded polytetrafluoroethylene graft, the three-layered scaffold significantly reduced platelet adhesion and hemolysis ratio compared to expanded polytetrafluoroethylene graft while comparable blood clotting profiles were observed for both electrospun scaffold and expanded polytetrafluoroethylene graft. However, inflammatory response to nanofibrous surface of the scaffold was reduced compared to expanded polytetrafluoroethylene graft. The electrospun scaffold also presented a significantly more supportive substrate for endothelialization than the expanded polytetrafluoroethylene graft. The results described herein suggested that the three-layered scaffold has superior biological properties compared to an expanded polytetrafluoroethylene graft for vascular tissue engineering.
authors.affiliation: Applied Physiology Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Shaghayegh
authors.initials: S
authors.lastname: Haghjooy Javanmard
authors.affiliation: Applied Physiology Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Jamal
authors.initials: J
authors.lastname: Anari
authors.affiliation: Department of Biomaterials, School of Advanced Medical Technology, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Anousheh
authors.initials: A
authors.lastname: Zargar Kharazi
authors.affiliation: Department of Cellulose and Paper Technology, Faculty of New Technologies and Energy Engineering, Shahid Beheshti University, Zirab Campus, Mazandaran, Iran e_vatankhah@sbu.ac.ir.
authors.forename: Elham
authors.initials: E
authors.lastname: Vatankhah
mesh_terms: D001672:Biocompatible Materials / Q000008:administration & dosage / Q000138:chemical synthesis; D001807:Blood Vessel Prosthesis; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000008:administration & dosage / Q000737:chemistry*; D042783:Endothelial Cells / Q000187:drug effects / Q000502:physiology*; D004867:Equipment Design; D006801:Humans; D008422:Materials Testing; D057139:Nanofibers / Q000008:administration & dosage / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011091:Polyesters / Q000008:administration & dosage / Q000737:chemistry*; D012399:Rotation; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; C016240:polycaprolactone; C033616:poly(lactide); D003094:Collagen
keywords: Vascular tissue engineering; endothelialization; hemocompatibility; inflammatory response; sequential electrospinning; three-layered tubular scaffold