title: Relevance of charge balance and hyaluronic acid on alginate-chitosan sponge microstructure and its influence on fibroblast growth.
abstract: The study of biomaterials by electrical charge scaling to explore the role of net charge on biocompatibility and suitability for tissue regeneration has been limited as has the search for products that could improve this first-rate variable. In the present study, we prepared sponges composed of chitosan/alginate (CS/ALG) with or without hyaluronic acid (HA) by mixing polymer stock solutions of different net electric charge ratios (n(+/) n(-) ), and then lyophilizing them to obtain porous materials. The electric charge ratios n(+/) n(-) studied were 0.3, 0.8, 1.0, and 2.5 for CS/ALG and 0.3, 1.0, 1.9, and 3.7 for CS/ALG/HA sponges. Under these conditions a role for net electric charge balance over sponge microstructure rearrangement, protection to dissolution, cellular proliferation, and cell-cell interactions was apparent, effects that were enhanced by copolymer modification with HA. Mass balance, electric charge, and specific products that influence both such as HA, have a potential in biomaterials for wound healing. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2537-2543, 2016.
authors.affiliation: Instituto de Ciencias Químicas, Facultad de Ciencias, Universidad Austral de Chile, Casilla 567, Valdivia, Chile.
authors.forename: Sandra L
authors.initials: SL
authors.lastname: Orellana
authors.affiliation: Instituto de Anatomía, Histología & Patología, Facultad de Medicina, Universidad Austral de Chile, Casilla 567, Valdivia, Chile.
authors.forename: Annesi
authors.initials: A
authors.lastname: Giacaman
authors.affiliation: Instituto de Anatomía, Histología & Patología, Facultad de Medicina, Universidad Austral de Chile, Casilla 567, Valdivia, Chile.
authors.forename: Francisca
authors.initials: F
authors.lastname: Pavicic
authors.affiliation: Instituto de Anatomía, Histología & Patología, Facultad de Medicina, Universidad Austral de Chile, Casilla 567, Valdivia, Chile.
authors.forename: Alejandra
authors.initials: A
authors.lastname: Vidal
authors.affiliation: Instituto de Ciencias Químicas, Facultad de Ciencias, Universidad Austral de Chile, Casilla 567, Valdivia, Chile.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Moreno-Villoslada
authors.affiliation: Instituto de Anatomía, Histología & Patología, Facultad de Medicina, Universidad Austral de Chile, Casilla 567, Valdivia, Chile.
authors.forename: Miguel
authors.initials: M
authors.lastname: Concha
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D048271:Chitosan / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006820:Hyaluronic Acid / Q000737:chemistry*; D051379:Mice; D016062:Porosity; D055672:Static Electricity
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D006820:Hyaluronic Acid; D048271:Chitosan
keywords: alginate; chitosan; electric charges balance; hyaluronic acid; sponge