title: Tetramethyl-6-carboxyrhodamine quenching-based aptasensing platform for aflatoxin B1: Analytical performance comparison of two aptamers.
abstract: In this study, a simple TAMRA (tetramethyl-6-carboxyrhodamine) quenching-based aptasensing platform was designed for the detection of aflatoxin B1 (AFB1). Here, we compared the analytical performance of two aptamer sequences: seqA and seqB. The AFB1 detection was based on the interactions of FAM (carboxyfluorescein)-labeled aptamer with TAMRA-labeled DNA complementary strand in the presence and absence of target analyte. Under optimized experimental conditions, TAMRA-labeled strand quenched the fluorescence response of FAM-labeled aptamer due to the noncovalent interaction between the two DNA strands. The binding of AFB1 induced the complex formation and weakened the interaction between FAM-labeled aptamer and TAMRA-labeled complementary strand, resulting in the fluorescence recovery. By using this principle concept, an assay was constructed for the detection of AFB1. The method exhibited good sensitivity, good selectivity with a limit of detection of 0.2 ng ml(-1), and a wide linear range from 0.25 to 32 ng ml(-1). For real sample application, the aptasensors were tested in beer and wine samples, with good recovery rates obtained for AFB1 detection.
authors.affiliation: BAE Laboratory, Université de Perpignan Via Domitia, Perpignan 66860, France; Department of Chemistry, National Institute of Technology, Warangal, Telangana, 506004, India.
authors.forename: K Yugender
authors.initials: KY
authors.lastname: Goud
authors.affiliation: BAE Laboratory, Université de Perpignan Via Domitia, Perpignan 66860, France; Biosensor Lab, Department of Chemistry, Birla Institute of Technology and Science (BITS), Pilani-K. K. Birla Goa Campus, Zuarinagar, Goa, 403726, India.
authors.forename: Atul
authors.initials: A
authors.lastname: Sharma
authors.affiliation: BAE Laboratory, Université de Perpignan Via Domitia, Perpignan 66860, France; Interdisciplinary Research Centre in Biomedical Materials (IRCBM), COMSATS Institute of Information Technology (CIIT), Lahore 54000, Pakistan.
authors.forename: Akhtar
authors.initials: A
authors.lastname: Hayat
authors.affiliation: BAE Laboratory, Université de Perpignan Via Domitia, Perpignan 66860, France.
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Catanante
authors.affiliation: Department of Chemistry, National Institute of Technology, Warangal, Telangana, 506004, India.
authors.forename: K Vengatajalabathy
authors.initials: KV
authors.lastname: Gobi
authors.affiliation: BAE Laboratory, Université de Perpignan Via Domitia, Perpignan 66860, France; LaborQ, University of Bucharest, 030018 Bucharest, Romania.
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Gurban
authors.affiliation: BAE Laboratory, Université de Perpignan Via Domitia, Perpignan 66860, France. Electronic address: jlmarty@univ-perp.fr.
authors.forename: Jean Louis
authors.initials: JL
authors.lastname: Marty
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis*; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001515:Beer / Q000032:analysis; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation* / Q000592:standards*; D005453:Fluorescence; D005524:Food Technology / Q000295:instrumentation* / Q000592:standards*; D057230:Limit of Detection; D012235:Rhodamines / Q000737:chemistry*; D013997:Time Factors; D014920:Wine / Q000032:analysis
chemical_list: D052157:Aptamers, Nucleotide; D012235:Rhodamines; D016604:Aflatoxin B1
keywords: Aflatoxin B1; Alcoholic beverages; FAM-labeled aptamer; Fluorescence quenching; TAMRA-labeled DNA strand