title: Human cytomegalovirus-encoded miR-US4-1 promotes cell apoptosis and benefits discharge of infectious virus particles by targeting QARS.
abstract: Human cytomegalovirus (HCMV) can cause congenital diseases and opportunistic infections in immunocompromised individuals. Its functional proteins and microRNAs (miRNAs) facilitate efficient viral propagation by altering host cell behaviour. Identification of functional target genes of miRNAs is an important step in studies on HCMV pathogenesis. In this study, Glutaminyl-tRNA Synthetase (QARS), which could regulate signal transduction pathways for cellular apoptosis, was identified as a direct target of hcmv-miR-US4-1. Apoptosis assay revealed that as silence of QARS by ectopic expression of hcmv-miR-US4-1 and specific small interference RNA of QARS can promote cell apoptosis in HCMV-infected HELF cells. Moreover, viral growth curve assays showed that hcmv-miR-US4-1 benefits the discharge of infectious virus particles. However, silence of hcmv-miR-US4-1 by its specific inhibitor overturned these effects. These results imply that hcmv-miR-US4-1 might have the same effects during HCMV nature infection. In general, hcmv-miR-US4-1 may involve in promoting cell apoptosis and benefiting discharge of infectious virus particles via down-regulation of QARS in HCMV-infected HELF cells.
authors.affiliation: Virus Laboratory, Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning 110004, China.
authors.forename: Yaozhong
authors.initials: Y
authors.lastname: Shao
authors.forename: Ying
authors.initials: Y
authors.lastname: Qi
authors.forename: Yujing
authors.initials: Y
authors.lastname: Huang
authors.forename: Zhongyang
authors.initials: Z
authors.lastname: Liu
authors.forename: Yanping
authors.initials: Y
authors.lastname: Ma
authors.forename: Xin
authors.initials: X
authors.lastname: Guo
authors.forename: Shujuan
authors.initials: S
authors.lastname: Jiang
authors.forename: Zhengrong
authors.initials: Z
authors.lastname: Sun
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ruan
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000096:biosynthesis / Q000235:genetics*; D017209:Apoptosis / Q000235:genetics; D003587:Cytomegalovirus / Q000235:genetics* / Q000472:pathogenicity; D003586:Cytomegalovirus Infections / Q000235:genetics* / Q000821:virology; D015967:Gene Expression Regulation, Viral; D054884:Host-Pathogen Interactions / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D014771:Virion / Q000235:genetics / Q000254:growth & development; D014779:Virus Replication / Q000235:genetics
chemical_list: D035683:MicroRNAs; C000615582:miR-US4-1, human cytomegalovirus; D000604:Amino Acyl-tRNA Synthetases; C020659:glutaminyl-tRNA synthetase