title: Effect of Surface Modifications of Ti40Zr10Cu38Pd12 Bulk Metallic Glass and Ti-6Al-4V Alloy on Human Osteoblasts In Vitro Biocompatibility.
abstract: The use of biocompatible materials, including bulk metallic glasses (BMGs), for tissue regeneration and transplantation is increasing. The good mechanical and corrosion properties of Ti40Zr10Cu38Pd12 BMG and its previously described biocompatibility makes it a potential candidate for medical applications. However, it is known that surface properties like topography might play an important role in regulating cell adhesion, proliferation and differentiation. Thus, in the present study, Ti40Zr10Cu38Pd12 BMG and Ti6-Al-4V alloy were surface-modified electrochemically (nanomesh) or physically (microscratched) to investigate the effect of material topography on human osteoblasts cells (Saos-2) adhesion, proliferation and differentiation. For comparative purposes, the effect of mirror-like polished surfaces was also studied. Electrochemical treatments led to a highly interconnected hierarchical porous structure rich in oxides, which have been described to improve corrosion resistance, whereas microscratched surfaces showed a groove pattern with parallel trenches. Cell viability was higher than 96% for the three topographies tested and for both alloy compositions. In all cases, cells were able to adhere, proliferate and differentiate on the alloys, hence indicating that surface topography plays a minor role on these processes, although a clear cell orientation was observed on microscratched surfaces. Overall, our results provide further evidence that Ti40Zr10Cu38Pd12 BMG is an excellent candidate, in the present two topographies, for bone repair purposes.
authors.affiliation: Departament de Biologia Cel·lular, Fisiologia i Immunologia, Universitat Autònoma de Barcelona, Edifici Cc, Bellaterra, Spain.
authors.forename: Andreu
authors.initials: A
authors.lastname: Blanquer
authors.affiliation: Departament de Física, Universitat Autònoma de Barcelona, Edifici Cc, Bellaterra, Spain.
authors.forename: Anna
authors.initials: A
authors.lastname: Hynowska
authors.affiliation: Departament de Biologia Cel·lular, Fisiologia i Immunologia, Universitat Autònoma de Barcelona, Edifici Cc, Bellaterra, Spain.
authors.forename: Carme
authors.initials: C
authors.lastname: Nogués
authors.affiliation: Departament de Biologia Cel·lular, Fisiologia i Immunologia, Universitat Autònoma de Barcelona, Edifici Cc, Bellaterra, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Ibáñez
authors.affiliation: Departament de Física, Universitat Autònoma de Barcelona, Edifici Cc, Bellaterra, Spain.|Institució Catalana de Recerca i Estudis Avançats, Barcelona, Spain.
authors.forename: Jordi
authors.initials: J
authors.lastname: Sort
authors.affiliation: Departament de Física, Universitat Autònoma de Barcelona, Edifici Cc, Bellaterra, Spain.
authors.forename: Maria Dolors
authors.initials: MD
authors.lastname: Baró
authors.affiliation: Multi-Scale Robotics Lab, Institute of Robotics and Intelligent Systems, ETH Zurich, Zurich, Switzerland.
authors.forename: Berna
authors.initials: B
authors.lastname: Özkale
authors.affiliation: Multi-Scale Robotics Lab, Institute of Robotics and Intelligent Systems, ETH Zurich, Zurich, Switzerland.
authors.forename: Salvador
authors.initials: S
authors.lastname: Pané
authors.affiliation: Departament de Física, Universitat Autònoma de Barcelona, Edifici Cc, Bellaterra, Spain.
authors.forename: Eva
authors.initials: E
authors.lastname: Pellicer
authors.affiliation: Departament de Biologia Cel·lular, Fisiologia i Immunologia, Universitat Autònoma de Barcelona, Edifici Cc, Bellaterra, Spain.
authors.forename: Leonardo
authors.identifier: http://orcid.org/0000-0002-6151-8503
authors.initials: L
authors.lastname: Barrios
mesh_terms: D000497:Alloys; D001672:Biocompatible Materials / Q000737:chemistry*; D001861:Bone Regeneration; D000069343:Bone-Implant Interface / Q000502:physiology*; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D055664:Electrochemical Techniques; D005898:Glass / Q000737:chemistry*; D006801:Humans; D016348:Osseointegration / Q000187:drug effects / Q000502:physiology*; D010006:Osteoblasts / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects*; D019736:Prostheses and Implants; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D000497:Alloys; D001672:Biocompatible Materials; C031462:titanium alloy (TiAl6V4); D014025:Titanium