title: Loss of heterozygosity and mRNA expression at deleted in colorectal cancer gene locus in gastric cancer.
abstract: OBJECTIVE
  To assess the effects of the deleted in colorectal cancer (DCC) gene changes on the development and progression of gastric cancer.
  
  
  METHODS
  The loss of heterozygosity (LOH) and mRNA expression DCC gastric cancer using a PCR-based detection method.
  
  
  RESULTS
  LOH was found in 35.3% (18/51) of the specimens, and the LOH was more frequently detected in tumors from patients with stage III or IV cancer (50.5%) than those in stages I or II (14.3%) (P < 0.05). The occurrence of LOH was not found to correlate with the histological type, tumor size, invasion depth and lymph node metastasis of gastric cancer. The mRNA expression of the DCC gene was studied in 26 of the 51 cases, of which LOE was found in 30.8% (8/26). LOE was not significantly correlated to LOH or other clinicopathological parameters.
  
  
  CONCLUSIONS
  LOH and LOE of DCC gene are frequently encountered in gastric cancer, and the LOH of DCC gene is a late event associated with progression of gastric cancer.
authors.affiliation: Dong-Xu Wang, Dian-Chun Fang, Yuan-Hui Luo, Wei-Wen Liu, Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing 630038, China.
authors.forename: D X
authors.initials: DX
authors.lastname: Wang
authors.affiliation: Dong-Xu Wang, Dian-Chun Fang, Yuan-Hui Luo, Wei-Wen Liu, Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing 630038, China.
authors.forename: D C
authors.initials: DC
authors.lastname: Fang
authors.affiliation: Dong-Xu Wang, Dian-Chun Fang, Yuan-Hui Luo, Wei-Wen Liu, Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing 630038, China.
authors.forename: Y H
authors.initials: YH
authors.lastname: Luo
authors.affiliation: Dong-Xu Wang, Dian-Chun Fang, Yuan-Hui Luo, Wei-Wen Liu, Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing 630038, China.
authors.forename: W W
authors.initials: WW
authors.lastname: Liu
keywords: DCC gene; Gene expression; Heterozygosity loss; Stomach neoplasms; mRNA