title: Ultrasensitive Detection of Low-Abundance Protein Biomarkers by Mass Spectrometry Signal Amplification Assay.
abstract: A mass spectrometry signal amplification method is developed for the ultrasensitive and selective detection of low-abundance protein biomarkers by utilizing tag molecules on gold nanoparticles (AuNPs). EpCAM and thrombin as model targets are captured by specific aptamers immobilized on the AuNPs. With laser desorption/ionization time-of-flight mass spectrometry (LDI-TOF MS), the mass tag molecules are detected to represent the protein biomarkers. Benefiting from the MS signal amplification, the assay can achieve a limit of detection of 100 aM. The method is further applied to detect thrombin in fetal bovine serum and EpCAM in cell lysates to demonstrate its selectivity and feasibility in complex biological samples. With the high sensitivity and specificity, the protocol shows great promise for providing a new route to single-cell analysis and early disease diagnosis.
authors.affiliation: Department of Chemistry, State Key Laboratory of Molecular Engineering of Polymers and Institutes of Biomedical Sciences, Fudan University , Handan Road 220, Shanghai 200433, China.
authors.forename: Ruijun
authors.initials: R
authors.lastname: Du
authors.affiliation: Department of Chemistry, State Key Laboratory of Molecular Engineering of Polymers and Institutes of Biomedical Sciences, Fudan University , Handan Road 220, Shanghai 200433, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Department of Chemistry, State Key Laboratory of Molecular Engineering of Polymers and Institutes of Biomedical Sciences, Fudan University , Handan Road 220, Shanghai 200433, China.
authors.forename: Jinrui
authors.initials: J
authors.lastname: Gan
authors.affiliation: Department of Chemistry, State Key Laboratory of Molecular Engineering of Polymers and Institutes of Biomedical Sciences, Fudan University , Handan Road 220, Shanghai 200433, China.
authors.forename: Yuning
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Chemistry, State Key Laboratory of Molecular Engineering of Polymers and Institutes of Biomedical Sciences, Fudan University , Handan Road 220, Shanghai 200433, China.|Shanghai Stomatological Hospital, Fudan University , East Beijing Road 356, Shanghai 200001, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Qiao
authors.affiliation: Department of Chemistry, State Key Laboratory of Molecular Engineering of Polymers and Institutes of Biomedical Sciences, Fudan University , Handan Road 220, Shanghai 200433, China.|Shanghai Stomatological Hospital, Fudan University , East Beijing Road 356, Shanghai 200001, China.
authors.forename: Baohong
authors.initials: B
authors.lastname: Liu
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry; D015415:Biomarkers / Q000032:analysis*; D002417:Cattle; D045744:Cell Line, Tumor; D000071858:Epithelial Cell Adhesion Molecule / Q000032:analysis* / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D051379:Mice; D044967:Serum / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013917:Thrombin / Q000032:analysis* / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D015415:Biomarkers; D000071858:Epithelial Cell Adhesion Molecule; D006046:Gold; D013917:Thrombin