title: Analysis of the association of HOTAIR single nucleotide polymorphism (rs920778) and risk of cervical cancer.
abstract: We recently demonstrated that overexpression of HOTAIR (Hox transcript antisense intergenic RNA) was associated with tumor progression and radio-resistance in human cervical cancer. Considering the single nucleotide polymorphism (SNP) rs920778 (C>T) could influence HOTAIR expression and cancer predisposition in other malignancies, we herein investigated the association between rs920778 status and cervical cancer susceptibility in a Chinese population. Using the specific TaqMan PCR assay, we genotyped rs920778 in 215 cervical cancer patients and 430 age-matched healthy controls. As shown in our data, TT genotype of rs920778 was significantly correlated with the upregulation of HOTAIR (p = 0.008). Compared with the healthy control, TT genotype and T allele notably indicated a much higher risk of cervical cancer [TT genotype: odds ratio (OR) = 2.186, 95% confidence interval (CI) = 1.378-3.466, p = 0.003; T allele: OR = 1.556, 95% CI = 1.221-1.981]. In addition, we also found that the TT genotype of rs920778 was correlated with advanced tumor stage (p = 0.039), highly histological grade (p = 0.013), lympho node metastasis (p < 0.001) and positive infection of high risk HPV (p < 0.001). Among the patients who underwent concurrent chemo-radiotherapy, TT genotype carriers present notably resistance to the combination of EBRT + ICBT + cisplatin (p = 0.023). In conclusion, we firstly reported that TT genotype of HOTAIR rs920778 was significantly associated with the cervical cancer susceptibility. Moreover, the TT genotype of rs920778 might be a potent prognostic marker in cervical cancer patients.
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.|Ovarian and Cervical Disease Clinical Treatment Center of Henan Province, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Qiu
authors.affiliation: Department of Gynecologic Oncology, The Affiliated Hospital of Jiangnan University and The Fourth People's Hospital of Wuxi, Wuxi, China.
authors.forename: Qiuli
authors.initials: Q
authors.lastname: Liu
authors.affiliation: School of Medicine, Jiangsu University, Zhenjiang, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.|Ovarian and Cervical Disease Clinical Treatment Center of Henan Province, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Gynecologic Oncology, The Affiliated Hospital of Jiangnan University and The Fourth People's Hospital of Wuxi, Wuxi, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.|Ovarian and Cervical Disease Clinical Treatment Center of Henan Province, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Zhongfu
authors.initials: Z
authors.lastname: Yuan
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Cancer Institute, Xuzhou Medical College, Xuzhou, China.
authors.forename: Dong-Sheng
authors.initials: DS
authors.lastname: Pei
mesh_terms: D000328:Adult; D000368:Aged; D000970:Antineoplastic Agents / Q000627:therapeutic use; D002681:China / Q000453:epidemiology; D004351:Drug Resistance; D005260:Female; D020022:Genetic Predisposition to Disease; D060005:Genotyping Techniques; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000145:classification / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D062085:RNA, Long Noncoding / Q000235:genetics*; D018570:Risk Assessment; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis / Q000453:epidemiology* / Q000473:pathology* / Q000821:virology
chemical_list: D000970:Antineoplastic Agents; C561566:HOTAIR long untranslated RNA, human; D062085:RNA, Long Noncoding
keywords: Cervical cancer; Hox transcript antisense intergenic RNA; cancer susceptibility; long non-coding RNA; rs920778 polymorphism