title: Assay of Endocannabinoid Uptake.
abstract: Endocannabinoids at physiological concentrations are crossing cellular membranes by facilitated diffusion, a process that can be studied by measuring transport kinetics. Here, we describe a radiosubstrate-based assay using arachidonoyl[1-(3)H]ethanolamine or arachidonoyl[1,2,3-(3)H]glycerol to measure the cellular endocannabinoid uptake in a three-phase assay with human U937 cells.
authors.affiliation: Institute of Biochemistry and Molecular Medicine, NCCR TransCure, University of Bern, Bern, Switzerland.
authors.forename: Mark
authors.initials: M
authors.lastname: Rau
authors.affiliation: Institute of Biochemistry and Molecular Medicine, NCCR TransCure, University of Bern, Bern, Switzerland.
authors.forename: Simon
authors.initials: S
authors.lastname: Nicolussi
authors.affiliation: Institute of Biochemistry and Molecular Medicine, NCCR TransCure, University of Bern, Bern, Switzerland.
authors.forename: Andrea
authors.initials: A
authors.lastname: Chicca
authors.affiliation: Institute of Biochemistry and Molecular Medicine, NCCR TransCure, University of Bern, Bern, Switzerland. gertsch@ibmm.unibe.ch.
authors.forename: Jürg
authors.initials: J
authors.lastname: Gertsch
mesh_terms: D001681:Biological Assay; D002462:Cell Membrane / Q000378:metabolism; D063388:Endocannabinoids / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D011874:Radiometry / Q000379:methods; D020298:U937 Cells
chemical_list: D063388:Endocannabinoids
keywords: 2-Arachidonoylglycerol; Anandamide; Endocannabinoid uptake