title: Ussing Chamber Technique to Measure Intestinal Epithelial Permeability.
abstract: Epithelial cells are polarized and have tight junctions that contribute to barrier function. Assessment of barrier function typically involves measurement of electrophysiological parameters or movement of nonionic particles across an epithelium. Here, we describe measurement of transepithelial electrical conductance or resistance, determination of dilution potential, and assessment of flux of nonionic particles such as dextran or mannitol, with particular emphasis on Ussing chamber techniques.
authors.affiliation: Department of Radiation Oncology, University of Florida Shands Cancer Center, Cancer and Genetics Research Complex, 2033 Mowry Road, Box 103633, Gainesville, FL, 32610, USA. vidyasagar@ufl.edu.
authors.forename: Sadasivan
authors.initials: S
authors.lastname: Vidyasagar
authors.affiliation: Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT, 06520-8026, USA.
authors.forename: Gordon
authors.initials: G
authors.lastname: MacGregor
mesh_terms: D000818:Animals; D016764:Cell Polarity; D004553:Electric Conductivity; D004847:Epithelial Cells / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism*; D010539:Permeability; D051381:Rats
keywords: Diffusion potential; Paracellular permeability; Tight junction; Transepithelial resistance