title: Transcriptome Signatures Reveal Rapid Induction of Immune-Responsive Genes in Human Memory CD8(+) T Cells.
abstract: Memory T cells (TM) play a prominent role in protection and auto-immunity due to their ability to mount a more effective response than naïve T cells (TN). However, the molecular mechanisms underlying enhanced functionality of TM are not well defined, particularly in human TM. We examined the global gene expression profiles of human CD8(+) TN and TM before and after stimulation. There were 1,284, 1,373 and 1,629 differentially expressed genes between TN and TM at 0 hr, 4 hr and 24 hr after stimulation, respectively, with more genes expressed to higher levels in TM. Genes rapidly up-regulated in TN cells were largely involved in nitrogen, nucleoside and amino acid metabolisms. In contrast, those in CD8(+) TM were significantly enriched for immune-response-associated processes, including cytokine production, lymphocyte activation and chemotaxis. Multiple cytokines were rapidly up-regulated in TM cells, including effector cytokines known to be produced by CD8(+) T cells and important for their functions, as well as regulatory cytokines, both pro- and anti-inflammatory, that are not typically produced by CD8(+) T cells. These results provide new insights into molecular mechanisms that contribute to the enhanced functionality of human CD8(+) TM and their prominent role in protection and auto-immunity.
authors.affiliation: Shanghai Institute of Immunology, Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Yang
authors.affiliation: Shanghai Institute of Immunology, Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors.forename: Asma
authors.initials: A
authors.lastname: Khanniche
authors.affiliation: Department of Microbiology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.
authors.forename: Joanna R
authors.initials: JR
authors.lastname: DiSpirito
authors.affiliation: Shanghai Institute of Immunology, Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Ji
authors.affiliation: Shanghai Institute of Immunology, Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors.forename: Shujun
authors.initials: S
authors.lastname: Wang
authors.affiliation: Shanghai Institute of Immunology, Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Shanghai Institute of Immunology, Shanghai Jiaotong University School of Medicine, Shanghai, China.|Department of Microbiology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.
authors.forename: Hao
authors.initials: H
authors.lastname: Shen
mesh_terms: D000596:Amino Acids / Q000276:immunology / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D002454:Cell Differentiation; D002633:Chemotaxis / Q000235:genetics / Q000276:immunology; D016207:Cytokines / Q000276:immunology / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000276:immunology*; D015894:Genome, Human / Q000276:immunology*; D006801:Humans; D007156:Immunologic Memory / Q000235:genetics*; D008213:Lymphocyte Activation; D046228:Microarray Analysis; D058977:Molecular Sequence Annotation; D009584:Nitrogen / Q000276:immunology / Q000378:metabolism; D009705:Nucleosides / Q000276:immunology / Q000378:metabolism; D061251:Primary Cell Culture; D059467:Transcriptome
chemical_list: D000596:Amino Acids; D016207:Cytokines; D009705:Nucleosides; D009584:Nitrogen