title: Pinocembrin attenuates gentamicin-induced nephrotoxicity in rats.
abstract: Oxidative stress mediated apoptosis of renal tubular cells is a major pathology of gentamicin-induced nephrotoxicity, which is one of the prevailing causes of acute renal failure. Pinocembrin is a major flavonoid found in rhizomes of fingerroot (Boesenbergia pandurata). It has pharmacological and biological activities including antimicrobial, anti-inflammatory, and antioxidant effects. Preclinical studies have suggested that pinocembrin protects rat brain and heart against oxidation and apoptosis induced by ischemia-reperfusion. The aim of the current study was to investigate the mechanisms of renoprotection elicited by pinocembrin in gentamicin-induced nephrotoxicity. Nephrotoxicity was induced in rats by intraperitoneal injection (i.p.) of gentamicin, and pinocembrin was administered via i.p. 30 min before gentamicin treatment for 10 days. Gentamicin-induced nephrotoxicity was indicated by the reduced renal function and renal Oat3 function and expression. Gentamicin treatment also stimulated Nrf2, HO-1, and NQO1, as well as the pro-apoptotic proteins Bax and caspase-3, concomitant with the attenuation of Bcl-XL expression in the renal cortical tissues. Pinocembrin pretreatment improved renal function and renal Oat3 function and reduced oxidative stress and apoptotic conditions. These findings indicate that pinocembrin has a protective effect against gentamicin-induced nephrotoxicity, which may be due in part to its antioxidant and anti-apoptotic effects, subsequently leading to improved renal function.
authors.affiliation: a Division of Physiology, School of Medical Sciences, University of Phayao, Phayao, Thailand.
authors.forename: Sasivimon
authors.initials: S
authors.lastname: Promsan
authors.affiliation: b Department of Physiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Krit
authors.initials: K
authors.lastname: Jaikumkao
authors.affiliation: b Department of Physiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Anchalee
authors.initials: A
authors.lastname: Pongchaidecha
authors.affiliation: c Cardiac Electrophysiology Research and Training Center, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Nipon
authors.initials: N
authors.lastname: Chattipakorn
authors.affiliation: d Department of Physiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
authors.forename: Varanuj
authors.initials: V
authors.lastname: Chatsudthipong
authors.affiliation: e Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Phatchawan
authors.initials: P
authors.lastname: Arjinajarn
authors.affiliation: f Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Lampang Rajabhat University, Lampang, Thailand.
authors.forename: Wilart
authors.initials: W
authors.lastname: Pompimon
authors.affiliation: b Department of Physiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Anusorn
authors.initials: A
authors.lastname: Lungkaphin
mesh_terms: D058186:Acute Kidney Injury / Q000139:chemically induced / Q000517:prevention & control*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity; D044950:Flavanones / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D005839:Gentamicins / Q000633:toxicity*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D020011:Protective Agents / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D030521:Zingiberaceae
chemical_list: D000900:Anti-Bacterial Agents; D044950:Flavanones; D005839:Gentamicins; D010936:Plant Extracts; D020011:Protective Agents; C016063:pinocembrin
keywords: apoptose; apoptosis; fonction rénale; gentamicin; gentamicine; nephrotoxicity; néphrotoxicité; organic anion transporter; oxidative stress; pinocembrin; pinocembrine; renal function; stress oxydatif; transporteur anionique organique