title: Design Defines the Effects of Nanoceria at a Low Dose on Soil Microbiota and the Potentiation of Impacts by the Canola Plant.
abstract: Soils act as nanoceria sinks via agricultural spreading and surface waters. Canola plants were grown for one month in soil spiked with nanoceria (1 mg·kg(-1)). To define the role of nanomaterials design on environmental impacts, we studied nanoceria with different sizes (3.5 or 31 nm) and coating (citrate). We measured microbial activities involved in C, N, and P cycling in the rhizosphere and unplanted soil. Bacterial community structure was analyzed in unplanted soil, rhizosphere, and plant roots by 454-pyrosequencing of the 16S rRNA gene. This revealed an impact gradient dependent on nanomaterials design, ranging from decreased microbial enzymatic activities in planted soil to alterations in bacterial community structure in roots. Particle size/aggregation was a key parameter in modulating nanoceria effects on root communities. Citrate coating lowered the impact on microbial enzymatic activities but triggered variability in the bacterial community structure near the plant root. Some nanoceria favored taxa whose closest relatives are hydrocarbon-degrading bacteria and disadvantaged taxa frequently associated in consortia with disease-suppressive activity toward plant pathogens. This work provides a basis to determine outcomes of nanoceria in soil, at a dose close to predicted environmental concentrations, and to design them to minimize these impacts.
authors.affiliation: Laboratory of Microbial Ecology of the Rhizosphere and Extreme Environments (LEMIRE), Aix-Marseille Université, CEA, CNRS, UMR 7265 Biosciences and Biotechnology Institute of Aix-Marseille (BIAM), ECCOREV FR 3098, CEA Cadarache, St-Paul-lez-Durance 13108, France.|GDRi iCEINT, International Consortium for the Environmental Implication of Nanotechnology, CNRS-Duke University , F-13545 Aix-en-Provence, France.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Hamidat
authors.affiliation: Laboratory of Microbial Ecology of the Rhizosphere and Extreme Environments (LEMIRE), Aix-Marseille Université, CEA, CNRS, UMR 7265 Biosciences and Biotechnology Institute of Aix-Marseille (BIAM), ECCOREV FR 3098, CEA Cadarache, St-Paul-lez-Durance 13108, France.|GDRi iCEINT, International Consortium for the Environmental Implication of Nanotechnology, CNRS-Duke University , F-13545 Aix-en-Provence, France.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Barakat
authors.affiliation: Laboratory of Microbial Ecology of the Rhizosphere and Extreme Environments (LEMIRE), Aix-Marseille Université, CEA, CNRS, UMR 7265 Biosciences and Biotechnology Institute of Aix-Marseille (BIAM), ECCOREV FR 3098, CEA Cadarache, St-Paul-lez-Durance 13108, France.|GDRi iCEINT, International Consortium for the Environmental Implication of Nanotechnology, CNRS-Duke University , F-13545 Aix-en-Provence, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Ortet
authors.affiliation: GDRi iCEINT, International Consortium for the Environmental Implication of Nanotechnology, CNRS-Duke University , F-13545 Aix-en-Provence, France.|Chimie de la Matière Condensée, UMR7574, Collège de France, Université de Jussieu , Paris F-75231, France.
authors.forename: Corinne
authors.initials: C
authors.lastname: Chanéac
authors.affiliation: GDRi iCEINT, International Consortium for the Environmental Implication of Nanotechnology, CNRS-Duke University , F-13545 Aix-en-Provence, France.|CNRS, Aix-Marseille Université, IRD, CEREGE UM34, F-13545 Aix en Provence, France.
authors.forename: Jérome
authors.initials: J
authors.lastname: Rose
authors.affiliation: GDRi iCEINT, International Consortium for the Environmental Implication of Nanotechnology, CNRS-Duke University , F-13545 Aix-en-Provence, France.|CNRS, Aix-Marseille Université, IRD, CEREGE UM34, F-13545 Aix en Provence, France.
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Bottero
authors.affiliation: Laboratory of Microbial Ecology of the Rhizosphere and Extreme Environments (LEMIRE), Aix-Marseille Université, CEA, CNRS, UMR 7265 Biosciences and Biotechnology Institute of Aix-Marseille (BIAM), ECCOREV FR 3098, CEA Cadarache, St-Paul-lez-Durance 13108, France.|GDRi iCEINT, International Consortium for the Environmental Implication of Nanotechnology, CNRS-Duke University , F-13545 Aix-en-Provence, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Heulin
authors.affiliation: Laboratory of Microbial Ecology of the Rhizosphere and Extreme Environments (LEMIRE), Aix-Marseille Université, CEA, CNRS, UMR 7265 Biosciences and Biotechnology Institute of Aix-Marseille (BIAM), ECCOREV FR 3098, CEA Cadarache, St-Paul-lez-Durance 13108, France.|GDRi iCEINT, International Consortium for the Environmental Implication of Nanotechnology, CNRS-Duke University , F-13545 Aix-en-Provence, France.
authors.forename: Wafa
authors.initials: W
authors.lastname: Achouak
authors.affiliation: Laboratory of Microbial Ecology of the Rhizosphere and Extreme Environments (LEMIRE), Aix-Marseille Université, CEA, CNRS, UMR 7265 Biosciences and Biotechnology Institute of Aix-Marseille (BIAM), ECCOREV FR 3098, CEA Cadarache, St-Paul-lez-Durance 13108, France.|GDRi iCEINT, International Consortium for the Environmental Implication of Nanotechnology, CNRS-Duke University , F-13545 Aix-en-Provence, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Santaella
mesh_terms: D064307:Microbiota; D018517:Plant Roots / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology
chemical_list: D012336:RNA, Ribosomal, 16S; D012987:Soil