title: Distribution pattern and genetic structure of Aedes zammitii (Diptera: Culicidae) along the Mediterranean and Aegean coasts of Turkey.
abstract: The Mariae species complex, consisting of Aedes mariae, Aedes phoeniciae, and Aedes zammitii, has a limited distribution worldwide. All three species are found in rocky habitats on the coastal areas of Mediterranean countries. Aedes phoeniciae and Ae. zammitii are two members of the Mariae complex that exist in Turkey. The aim of this study was to determine the distribution pattern and genetic structure of Ae. zammitii along the Mediterranean and Aegean regions. For this purpose, larval and adult samples of Ae. zammitii were collected from 19 different rocky habitats along the coastal regions of Antalya, Muğla, Aydın, İzmir, Balıkesir, and Çanakkale provinces. DNA isolation was performed primarily from collected samples, and mitochondrial NADH dehydrogenase 4 (ND4) gene was amplified by polymerase chain reaction. Based on ND4 sequence analyses, 21 haplotypes were detected along the distribution range of the species. Analyses of molecular variance (AMOVA) and spatial analyses of molecular variance (SAMOVA) indicated six groups, and most of the variation was among groups, demonstrating the population structuring at group level. Isolation by distance analyses (IBD) showed a correlation between geographic and genetic distances.
authors.affiliation: Arts and Science Faculty, Department of Biology, Adnan Menderes University, 09010, Aydın, Turkey. sarecihangir@adu.edu.tr.
authors.forename: Sare Ilknur
authors.initials: SI
authors.lastname: Yavasoglu
authors.affiliation: Arts and Science Faculty, Department of Biology, Adnan Menderes University, 09010, Aydın, Turkey.
authors.forename: Fatih Mehmet
authors.initials: FM
authors.lastname: Simsek
authors.affiliation: Arts and Science Faculty, Department of Biology, Adnan Menderes University, 09010, Aydın, Turkey.
authors.forename: Celal
authors.initials: C
authors.lastname: Ulger
mesh_terms: D000330:Aedes / Q000235:genetics*; D063147:Animal Distribution; D000818:Animals; D004272:DNA, Mitochondrial / Q000235:genetics; D014644:Genetic Variation; D006239:Haplotypes; D014421:Turkey
chemical_list: D004272:DNA, Mitochondrial
keywords: Aedes zammitii; Mariae complex; Mediterranean; Mitochondrial DNA; ND4; population genetics