title: Dynamic monitoring of platelet activation and its role in post-dissection inflammation in a canine model of acute type A aortic dissection.
abstract: BACKGROUND
  To confirm the activation of platelets (PLT) and explore the role of activated PLT in post-dissection inflammation.
  
  
  METHODS
  An acute type A aortic dissection (AAD) canine model was established. Mean platelet volume/platelet count (MPV/PTC), platelet size distribution width (PDW), and inflammatory cytokines (tumor necrosis factor-α [TNF-α] and interleukin-6 [IL-6]) were measured between anesthetization and thoracotomy (T1), at the end of the operation (T2), and at 2 h (T3), 4 h (T4), and 6 h (T5) after the operation. Bivariate analysis was used to determine the correlations between the peak MPV/PTC, PDW, and inflammatory cytokines at T4.
  
  
  RESULTS
  An AAD canine model was successfully established. Both MPV/PTC and PDW values were significantly higher at T3-T5 than at T1 (P < 0.05). Both were also significantly higher at T3-T5 in the dissection group than in the sham operation (SO) group (P < 0.05). Inflammatory cytokine levels were remarkably higher at T3-T5 than at T1, and were higher at T3-T5 in both the dissection and the SO group (P < 0.05). Bivariate analysis demonstrated positive correlations between MPV/PTC and both TNF-α (r = 0.826, P = 0.011) and IL-6 (r = 0.806, P = 0.016).
  
  
  CONCLUSIONS
  Activated PLT were identified after AAD, and played a critical role in the initiation of post-dissection inflammation.
authors.affiliation: Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Lane outside the southern No. 37, Cheng du, Sichuan, People's Republic of China.
authors.forename: Chaoyi
authors.initials: C
authors.lastname: Qin
authors.affiliation: Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Lane outside the southern No. 37, Cheng du, Sichuan, People's Republic of China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Lane outside the southern No. 37, Cheng du, Sichuan, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Gu
authors.affiliation: Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Lane outside the southern No. 37, Cheng du, Sichuan, People's Republic of China.
authors.forename: Zhenghua
authors.initials: Z
authors.lastname: Xiao
authors.affiliation: Department of Radiology, West China Hospital, Sichuan University, Lane outside the southern No. 37, Cheng du, Sichuan, People's Republic of China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Lane outside the southern No. 37, Cheng du, Sichuan, People's Republic of China. hxcardio@sina.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Meng
mesh_terms: D000784:Aortic Dissection / Q000097:blood / Q000473:pathology*; D000818:Animals; D017545:Aortic Aneurysm, Thoracic / Q000097:blood / Q000473:pathology*; D004195:Disease Models, Animal; D004285:Dogs; D007249:Inflammation / Q000097:blood / Q000473:pathology*; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D063847:Mean Platelet Volume; D015539:Platelet Activation; D010976:Platelet Count; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha
keywords: Acute type A aortic dissection; Inflammation; Platelet activation