title: Nucleoside Inhibitors of Zika Virus.
abstract: There is growing evidence that Zika virus (ZIKV) can cause devastating infant brain defects and other neurological disorders in humans. However, no specific antiviral therapy is available at present. We tested a series of 2'-C- or 2'-O-methyl-substituted nucleosides, 2'-C-fluoro-2'-C-methyl-substituted nucleosides, 3'-O-methyl-substituted nucleosides, 3'-deoxynucleosides, derivatives with 4'-C-azido substitution, heterobase-modified nucleosides, and neplanocins for their ability to inhibit ZIKV replication in cell culture. Antiviral activity was identified when 2'-C-methylated nucleosides were tested, suggesting that these compounds might represent promising lead candidates for further development of specific antivirals against ZIKV.
authors.affiliation: Department of Virology, Veterinary Research Institute, Brno.
authors.forename: Luděk
authors.initials: L
authors.lastname: Eyer
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Prague.
authors.forename: Radim
authors.initials: R
authors.lastname: Nencka
authors.affiliation: Department of Virology, Veterinary Research Institute, Brno.
authors.forename: Ivana
authors.initials: I
authors.lastname: Huvarová
authors.affiliation: Department of Virology, Veterinary Research Institute, Brno Institute of Parasitology, Biology Center of the Czech Academy of Sciences Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Palus
authors.affiliation: National Institute of Health Dr Ricardo Jorge-CEVDI/INSA, Águas de Moura, Portugal.
authors.forename: Maria
authors.initials: M
authors.lastname: Joao Alves
authors.affiliation: Aix Marseille Université, IRD French Institute of Research for Development, EHESP French School of Public Health, EPV UMR_D 190 Emergence des Pathologies Virales, France.
authors.forename: Ernest A
authors.initials: EA
authors.lastname: Gould
authors.affiliation: Rega Institute for Medical Research, KU Leuven, Belgium.
authors.forename: Erik
authors.initials: E
authors.lastname: De Clercq
authors.affiliation: Department of Virology, Veterinary Research Institute, Brno Institute of Parasitology, Biology Center of the Czech Academy of Sciences Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic.
authors.forename: Daniel
authors.initials: D
authors.lastname: Růžek
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002522:Chlorocebus aethiops; D008826:Microbial Sensitivity Tests; D009705:Nucleosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D014709:Vero Cells; D014779:Virus Replication / Q000187:drug effects*; D000071244:Zika Virus / Q000187:drug effects* / Q000502:physiology*
chemical_list: D000998:Antiviral Agents; D009705:Nucleosides
keywords: Zika virus; antiviral; flavivirus; nucleoside analogue; therapy