title: The Feasibility of Thermophilic Caldimonas manganoxidans as a Platform for Efficient PHB Production.
abstract: Recently, poly(3-hydroxybutyrate) (PHB) has been found in a few thermophilic strains where several advantages can be gained from running fermentation at high temperatures. Caldimonas manganoxidans, a thermophilic gram-negative bacterium, was investigated for the feasibility as a PHB-producing strain. It is suggested that the best fermentation strategy for achieving the highest PHB concentration of 5.4 ± 1.1 g/L (from 20 g/L glucose) in 24 h is to use the fermentation conditions that are favored for the bacterial growth, yet temperature and pH should be chosen at conditions that are favored for the PHB content. Besides, the above fermentation conditions produce PHB that has a high molecular weight of 1274 kDa with a low polydispersity index (PDI) of 1.45, where the highest Mw of PHB of 1399 kDa (PDI of 1.32) is obtained in this study. To the best knowledge of authors, C. manganoxidans has the best PHB productivity among the thermophiles and is comparable to those common PHB-producing mesophiles.
authors.affiliation: Department of Chemical Engineering, National Chung Hsing University, Taichung 402, Taiwan.
authors.forename: Li-Jung
authors.initials: LJ
authors.lastname: Hsiao
authors.affiliation: Department of Chemical Engineering, National Chung Hsing University, Taichung 402, Taiwan.
authors.forename: Ji-Hong
authors.initials: JH
authors.lastname: Lin
authors.affiliation: Department of Chemical Engineering, National Chung Hsing University, Taichung 402, Taiwan.
authors.forename: Pantitra
authors.initials: P
authors.lastname: Sankatumvong
authors.affiliation: Department of Materials Science and Engineering, National Chung Hsing University, Taichung 402, Taiwan.
authors.forename: Tzong-Ming
authors.initials: TM
authors.lastname: Wu
authors.affiliation: Department of Chemical Engineering, National Chung Hsing University, Taichung 402, Taiwan. syli@dragon.nchu.edu.tw.
authors.forename: Si-Yu
authors.initials: SY
authors.lastname: Li
mesh_terms: D018533:Biomass; D002244:Carbon / Q000494:pharmacology; D042621:Comamonadaceae / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D005240:Feasibility Studies; D005947:Glucose / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D006885:Hydroxybutyrates / Q000378:metabolism*; D008970:Molecular Weight; D009584:Nitrogen / Q000494:pharmacology; D010100:Oxygen / Q000494:pharmacology; D011091:Polyesters / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013997:Time Factors; D000094122:Polyhydroxybutyrates
chemical_list: D002244:Carbon; D005947:Glucose; D006885:Hydroxybutyrates; D009584:Nitrogen; D010100:Oxygen; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates
keywords: Caldimonas manganoxidans; Glucose; Molecular weight; Poly(3-hydroxybutyrate) (PHB); Substrate inhibition; Thermophiles