title: EIYMNVPV Motif is Essential for A1CF Nucleus Localization and A1CF (-8aa) Promotes Proliferation of MDA-MB-231 Cells via Up-Regulation of IL-6.
abstract: Apobec-1 complementation factor (A1CF) is a heterogeneous nuclear ribonuceloprotein (hnRNP) and mediates apolipoprotein-B mRNA editing. A1CF can promote the regeneration of the liver by post-transcriptionally stabilizing Interleukin-6 (IL-6) mRNA. It also contains two transcriptional variants-A1CF64 and A1CF65, distinguished by the appearance of a 24-nucleotide motif which contributes to the corresponding eight-amino acid motif of EIYMNVPV. For the first time, we demonstrated that the EIYMNVPV motif was essential for A1CF nucleus localization, A1CF deficient of the EIYMNVPV motif, A1CF (-8aa) showed cytoplasm distribution. More importantly, we found that A1CF (-8aa), but not its full-length counterpart, can promote proliferation of MDA-MB-231 cells accompanied with increased level of IL-6 mRNA. Furthermore, silencing of IL-6 attenuated A1CF (-8aa)-induced proliferation in MDA-MB-231 cells. In conclusion, notably, these findings suggest that A1CF (-8aa) promoted proliferation of MDA-MB-231 cells in vitro viewing IL-6 as a target. Thus, the EIYMNVPV motif could be developed as a potential target for basal-like breast cancer therapy.
authors.affiliation: The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. shmily525520@sina.com.
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.affiliation: The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. lanyxiu@163.com.
authors.forename: Jin
authors.initials: J
authors.lastname: Hao
authors.affiliation: The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. yyokyy1126@hotmail.com.
authors.forename: Yue
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. pengrui911@foxmail.com.
authors.forename: Rui
authors.initials: R
authors.lastname: Peng
authors.affiliation: Laboratory of Organ Fibrosis Prophylaxis and Treatment by Combine Traditional Chinese and Western Medicine, Research Center of Combine Traditional Chinese and Western Medicine, Affiliated Traditional Medicine Hospital of Sichuan Medical University, Luzhou 646000, China. hackie_wang@126.com.
authors.forename: Honglian
authors.initials: H
authors.lastname: Wang
authors.affiliation: The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. cqmunds@163.com.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Ni
authors.affiliation: The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. guyupinglittle@sina.com.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Gu
authors.affiliation: The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. lyhuang0603@sina.com.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Huang
authors.affiliation: The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. mao1204086118@163.com.
authors.forename: Zhaomin
authors.initials: Z
authors.lastname: Mao
authors.affiliation: The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. Zhongshilyu@163.com.
authors.forename: Zhongshi
authors.initials: Z
authors.lastname: Lyu
authors.affiliation: The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. duyao_steve@163.com.
authors.forename: Yao
authors.initials: Y
authors.lastname: Du
authors.affiliation: The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. liuzhicheng323@163.com.
authors.forename: Zhicheng
authors.initials: Z
authors.lastname: Liu
authors.affiliation: The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. 18715854036@sina.cn.
authors.forename: Yiman
authors.initials: Y
authors.lastname: Li
authors.affiliation: The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. 18883936591@163.com.
authors.forename: Pan
authors.initials: P
authors.lastname: Ju
authors.affiliation: The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. lys960110@sina.com.
authors.forename: Yaoshui
authors.initials: Y
authors.lastname: Long
authors.affiliation: The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. keithljn@sina.cn.
authors.forename: Jianing
authors.initials: J
authors.lastname: Liu
authors.affiliation: The Division of Molecular Nephrology and the Creative Training Center for Undergraduates, The M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China. zhouqin@cqmu.edu.cn.
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhou
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism*; D049109:Cell Proliferation; D003593:Cytoplasm / Q000378:metabolism; D004285:Dogs; D006801:Humans; D015850:Interleukin-6 / Q000235:genetics*; D061985:Madin Darby Canine Kidney Cells; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: C411647:A1CF protein, human; C508600:IL6 protein, human; D015850:Interleukin-6; D016601:RNA-Binding Proteins
keywords: Apobec-1 complementation factor (A1CF); EIYMNVPV motif; Interleukin-6 (IL-6); MDA-MB-231 cells; nucleus localization; proliferation