title: Polyamide 6/chitosan nanofibers as support for the immobilization of Trametes versicolor laccase for the elimination of endocrine disrupting chemicals.
abstract: In recent years, there has been an increase in efforts to improve wastewater treatment as the concentration of dangerous pollutants, such as endocrine disrupting chemicals, in wastewater increases. These compounds, which mimic the effect of hormones, have a negative impact on human health and are not easily removed from water. One way to effectively eliminate these pollutants is to use enzymatically activated materials. In this study, we report on the use of laccase from the white rot fungus Trametes versicolor immobilized onto polyamide 6/chitosan (PA6/CHIT) nanofibers modified using two different spacers (bovine serum albumin and hexamethylenediamine). We then tested the ability of the PA6/CHIT-laccase biocatalysts to eliminate a mixture containing 50μM of two endocrine disrupting chemicals: bisphenol A and 17α-ethinylestradiol. The PA6/CHIT nanofiber matrix used in this study not only proved to be a suitable carrier for immobilized and modified laccase but was also efficient in the removal of a mixture of endocrine disrupting chemicals in three treatment cycles.
authors.affiliation: Institute for Nanomaterials, Advanced Technologies and Innovation, Technical University of Liberec, 46117 Liberec, Czech Republic; Faculty of Mechatronics, Informatics and Interdisciplinary Studies, Technical University of Liberec, 46117 Liberec, Czech Republic.
authors.forename: Milena
authors.initials: M
authors.lastname: Maryšková
authors.affiliation: Earth and Life Institute, Université catolique de Louvain, 1348 Louvain-la-Neuve, Belgium; Center for Research in Molecular Medicine and Chronic Diseases-CiMUS, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain.
authors.forename: Inés
authors.initials: I
authors.lastname: Ardao
authors.affiliation: Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain.
authors.forename: Carlos A
authors.initials: CA
authors.lastname: García-González
authors.affiliation: Institute for Nanomaterials, Advanced Technologies and Innovation, Technical University of Liberec, 46117 Liberec, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Martinová
authors.affiliation: Institute for Nanomaterials, Advanced Technologies and Innovation, Technical University of Liberec, 46117 Liberec, Czech Republic; Faculty of Mechatronics, Informatics and Interdisciplinary Studies, Technical University of Liberec, 46117 Liberec, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Rotková
authors.affiliation: Institute for Nanomaterials, Advanced Technologies and Innovation, Technical University of Liberec, 46117 Liberec, Czech Republic; Faculty of Mechatronics, Informatics and Interdisciplinary Studies, Technical University of Liberec, 46117 Liberec, Czech Republic. Electronic address: alena.sevcu@tul.cz.
authors.forename: Alena
authors.initials: A
authors.lastname: Ševců
mesh_terms: D001673:Biodegradation, Environmental; D002209:Caprolactam / Q000031:analogs & derivatives / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry; D052244:Endocrine Disruptors / Q000378:metabolism*; D004800:Enzymes, Immobilized / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism*; D006801:Humans; D042845:Laccase / Q000378:metabolism*; D057139:Nanofibers / Q000737:chemistry / Q000648:ultrastructure; D011108:Polymers / Q000737:chemistry; D055454:Trametes / Q000201:enzymology*
chemical_list: D052244:Endocrine Disruptors; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D011108:Polymers; C009916:nylon 6; D002209:Caprolactam; D048271:Chitosan; D042845:Laccase
keywords: -ethinylestradiol; 17α; Bisphenol A; Laccase immobilization; Nanofibers