title: Preliminary study on the loss of heterozygosity at 17p13 in gastric and colorectal cancers.
abstract: OBJECTIVE
  To evaluate the role of p53 in the development and progression of colorectal cancer and gastric carcinoma by analyzing the loss of heterozygosity (LOH) at 17p13.1 and 17p13.3.
  
  
  METHODS
  LOH at the p53 gene locus and 17p13.3 were examined in 22 cases of gastric carcinoma and 14 cases of colorectal cancer by Southern blot analysis.
  
  
  RESULTS
  Of the 22 gastrocarcinoma cases, 12 (54%) were heterozygous and LOH was detected in 6 (50%) of the 12 informative cases. In the 14 colorectal cancer cases, 10 (71%) were heterozygous, and LOH was detected in 6 (60%) of the 10 informative cases.
  
  
  CONCLUSIONS
  LOH at the p53 gene locus is a frequent event in multiple step carcinogenesis progression. The high frequency of LOH at 17p13.3 suggests that there may be another tumor suppresser gene in that chromosome region.
authors.affiliation: Guo-Jun Wu, Shao-Lin Shi, Qi-Ping Zheng, Long Yu, Shou-Yuan Zhao, Institute of Genetics, Fudan University, Shanghai 200433, China.
authors.forename: G J
authors.initials: GJ
authors.lastname: Wu
authors.affiliation: Guo-Jun Wu, Shao-Lin Shi, Qi-Ping Zheng, Long Yu, Shou-Yuan Zhao, Institute of Genetics, Fudan University, Shanghai 200433, China.
authors.forename: X N
authors.initials: XN
authors.lastname: Shan
authors.affiliation: Guo-Jun Wu, Shao-Lin Shi, Qi-Ping Zheng, Long Yu, Shou-Yuan Zhao, Institute of Genetics, Fudan University, Shanghai 200433, China.
authors.forename: M F
authors.initials: MF
authors.lastname: Li
authors.affiliation: Guo-Jun Wu, Shao-Lin Shi, Qi-Ping Zheng, Long Yu, Shou-Yuan Zhao, Institute of Genetics, Fudan University, Shanghai 200433, China.
authors.forename: S L
authors.initials: SL
authors.lastname: Shi
authors.affiliation: Guo-Jun Wu, Shao-Lin Shi, Qi-Ping Zheng, Long Yu, Shou-Yuan Zhao, Institute of Genetics, Fudan University, Shanghai 200433, China.
authors.forename: Q P
authors.initials: QP
authors.lastname: Zheng
authors.affiliation: Guo-Jun Wu, Shao-Lin Shi, Qi-Ping Zheng, Long Yu, Shou-Yuan Zhao, Institute of Genetics, Fudan University, Shanghai 200433, China.
authors.forename: L
authors.initials: L
authors.lastname: Yu
authors.affiliation: Guo-Jun Wu, Shao-Lin Shi, Qi-Ping Zheng, Long Yu, Shou-Yuan Zhao, Institute of Genetics, Fudan University, Shanghai 200433, China.
authors.forename: S Y
authors.initials: SY
authors.lastname: Zhao
keywords: Colorectal neoplasms; Genes, suppressor, tumor; Heterozygosity loss; Stomach neoplasms; p53 gene