title: [The effects of graphene quantum dots on hematopoietic system in rats].
abstract: OBJECTIVE
  To study the effects of graphene quantum dots (GQDs) on hematopoietic system in rats.
  
  
  METHODS
  Thirty male SD rats were randomly divided into three groups (n = 10): control group, high dose group (10 mg/kg · d), low dose group (5 mg/kg · d), The rats in experimental group were intravenous injected with GQDs for 28 days and those in control group were injected with normal saline at the same volume. Routine blood and the function of liver and kidney were detected by instrument analysis. The cycle and apoptosis of bone marrow mononuclear cells (BMCs) were detected by FCM. The other three only healthy male SD rat bone marrow mononuclear cells (BMCs) were cultured by joining GQDs for 24 h, 48 h,72 h in vitro, the proliferation was assayed by CCK-8, the content of granulocyte macrophage colony stimulating factor (GM-CSF) from cultural supernatants were detected by ELISA.
  
  
  RESULTS
  The amount of red blood cell and concentration of hemoglobin from experimental group were increased significantly compared with those of control groups (P < 0.05), the concentration of triglyceride and high density lipoprotein were decreased. DNA synthesis period was prolonged (P < 0.01), there was no significant difference in apoptosis. BMCs were promoted proliferation clearly after using GQDs for 72 h (P < 0.05). The content of GM-CSF was increased (P < 0.01) .
  
  
  CONCLUSIONS
  GQDs may promote hematopoietic function in rats.
authors.forename: Ting-jian
authors.initials: TJ
authors.lastname: Wang
authors.forename: Sha-li
authors.initials: SL
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis; D001854:Bone Marrow Cells / Q000187:drug effects*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D006108:Graphite / Q000494:pharmacology*; D006410:Hematopoiesis / Q000187:drug effects*; D008297:Male; D045663:Quantum Dots / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D006108:Graphite; D016178:Granulocyte-Macrophage Colony-Stimulating Factor