title: Complement factors alter the amount of PrP(Sc) in primary-cultured mouse cortical neurons associated with increased membrane permeability.
abstract: We examined the effects of complement factors on primary-cultured neurons infected with prions. The amount of protease K (PK)-resistant abnormal form of prion protein (PrP(Sc)) reached a maximum level at 12 and 16 days post exposure (dpe) in 22L- and Chandler-infected neurons, respectively. In Chandler-infected neurons, the reaction of complement factors C1q, C3 and C9 significantly increased membrane permeability. This was followed by a decrease of PK-resistant PrP(Sc) at 16 and 20dpe. In contrast, in 22L-infected neurons, the effects of complement factors were observed at 12 and 16dpe, but not at 20dpe. Membrane permeability also increased in 22L-infected neurons by reaction of complement factor C3, but interestingly, the amount of PK-resistant PrP(Sc) initially decreased, and then increased. These results suggest that the reactivity of complement factors in prion-infected neurons depends on the amount of PrP(Sc) and the prion strain.
authors.affiliation: Laboratory of Veterinary Hygiene, Graduate School of Veterinary Medicine, Hokkaido University, Nishi 9, Kita 18, Kita-ku, Sapporo 060-0818, Japan.
authors.forename: Rie
authors.initials: R
authors.lastname: Hasebe
authors.affiliation: Laboratory of Veterinary Hygiene, Graduate School of Veterinary Medicine, Hokkaido University, Nishi 9, Kita 18, Kita-ku, Sapporo 060-0818, Japan.
authors.forename: Misaki
authors.initials: M
authors.lastname: Tanaka
authors.affiliation: Laboratory of Veterinary Hygiene, Graduate School of Veterinary Medicine, Hokkaido University, Nishi 9, Kita 18, Kita-ku, Sapporo 060-0818, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Suzuki
authors.affiliation: Laboratory of Veterinary Hygiene, Graduate School of Veterinary Medicine, Hokkaido University, Nishi 9, Kita 18, Kita-ku, Sapporo 060-0818, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yamasaki
authors.affiliation: Laboratory of Veterinary Hygiene, Graduate School of Veterinary Medicine, Hokkaido University, Nishi 9, Kita 18, Kita-ku, Sapporo 060-0818, Japan. Electronic address: horiuchi@vetmed.hokudai.ac.jp.
authors.forename: Motohiro
authors.initials: M
authors.lastname: Horiuchi
mesh_terms: D000818:Animals; D016923:Cell Death; D002463:Cell Membrane Permeability / Q000276:immunology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D003165:Complement System Proteins / Q000276:immunology*; D051379:Mice; D009474:Neurons / Q000378:metabolism*; D018620:PrPSc Proteins / Q000378:metabolism*; D011328:Prions / Q000276:immunology / Q000378:metabolism
chemical_list: D018620:PrPSc Proteins; D011328:Prions; D003165:Complement System Proteins
keywords: Complement factor; Membrane permeability; Neuropathogenesis; Primary-cultured neuron; Prion disease