title: Interplay between mitogen-activated protein kinase and nitric oxide in brassinosteroid-induced pesticide metabolism in Solanum lycopersicum.
abstract: Nitric oxide (NO) and mitogen-activated protein kinase (MPK) play important roles in brassinosteroid (BR)-induced stress tolerance, however, their functions in BR-induced pesticides metabolism remain unclear. Here, we showed that MPK activity and transcripts of SlMPK1 and SlMPK2 were induced by chlorothalonil (CHT), a widely used fungicide, in tomato leaves. However, cosilencing of SlMPK1/2 compromised the 24-epibrassinolide (EBR)-induced upregulation of detoxification genes and CHT metabolism in tomato leaves. In addition, cosilencing of SlMPK1/2 inhibited the accumulation of S-nitrosothiol (SNO), the reservoir of nitric oxide (NO) in plants, whereas tungstate, the inhibitor of nitrate reductase (NR), blocked EBR-induced SNO accumulation and MPK activity. Inhibiting the accumulation of NO by cPTIO, the specific scavenger and tungstate abolished the EBR-induced upregulation of detoxification genes, glutathione accumulation and CHT metabolism. The results showed that MPK and NR-dependent NO were involved in BR-induced CHT metabolism. Notably, there was a positive crosstalk between the MPK and NO production.
authors.affiliation: Department of Horticulture, Zijingang Campus, Zhejiang University, Hangzhou 310058, China.
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Yin
authors.affiliation: Department of Horticulture, Zijingang Campus, Zhejiang University, Hangzhou 310058, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Horticulture, Zijingang Campus, Zhejiang University, Hangzhou 310058, China.
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Zhou
authors.affiliation: Department of Horticulture, Zijingang Campus, Zhejiang University, Hangzhou 310058, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Shi
authors.affiliation: Department of Horticulture, Zijingang Campus, Zhejiang University, Hangzhou 310058, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Institute of Pesticide & Environmental Toxicology, Zijingang Campus, Zhejiang University, Hangzhou 310058, China.
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Department of Horticulture, Zijingang Campus, Zhejiang University, Hangzhou 310058, China; Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Hangzhou 310058, China.
authors.forename: Jing-Quan
authors.initials: JQ
authors.lastname: Yu
authors.affiliation: Department of Horticulture, Zijingang Campus, Zhejiang University, Hangzhou 310058, China. Electronic address: xiaojianxia@zju.edu.cn.
authors.forename: Xiao-Jian
authors.initials: XJ
authors.lastname: Xia
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000378:metabolism; D060406:Brassinosteroids / Q000737:chemistry; D002734:Chlorophyll / Q000737:chemistry; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D020868:Gene Silencing; D005978:Glutathione / Q000378:metabolism; D018551:Solanum lycopersicum / Q000378:metabolism*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D050901:Nitrate Reductase / Q000737:chemistry*; D009569:Nitric Oxide / Q000737:chemistry*; D010575:Pesticides / Q000737:chemistry*; D018515:Plant Leaves / Q000378:metabolism
chemical_list: D060406:Brassinosteroids; D010575:Pesticides; D002734:Chlorophyll; D009569:Nitric Oxide; D000445:Aldehyde Oxidoreductases; C087006:formaldehyde dehydrogenase, glutathione-independent; D050901:Nitrate Reductase; D020928:Mitogen-Activated Protein Kinases; D005978:Glutathione
keywords: Chlorothalonil; Glutathione; Mitogen-activated protein kinase; Nitric oxide; Solanum lycopersicum