title: Avian reovirus σA and σNS proteins activate the phosphatidylinositol 3-kinase-dependent Akt signalling pathway.
abstract: The present study was conducted to identify avian reovirus (ARV) proteins that can activate the phosphatidylinositol 3-kinase (PI3K)-dependent Akt pathway. Based on ARV protein amino acid sequence analysis, σA, σNS, μA, μB and μNS were identified as putative proteins capable of mediating PI3K/Akt pathway activation. The recombinant plasmids σA-pcAGEN, σNS-pcAGEN, μA-pcAGEN, μB-pcAGEN and μNS-pcAGEN were constructed and used to transfect Vero cells, and the expression levels of the corresponding genes were quantified by immunofluorescence and Western blot analysis. Phosphorylated Akt (P-Akt) levels in the transfected cells were measured by flow cytometry and Western blot analysis. The results showed that the σA, σNS, μA, μB and μNS genes were expressed in Vero cells. σA-expressing and σNS-expressing cells had higher P-Akt levels than negative control cells, pcAGEN-expressing cells and cells designed to express other proteins (i.e., μA, μB and μNS). Pre-treatment with the PI3K inhibitor LY294002 inhibited Akt phosphorylation in σA- and σNS-expressing cells. These results indicate that the σA and σNS proteins can activate the PI3K/Akt pathway.
authors.affiliation: Department of Biotechnology, Guangxi Key Laboratory of Animal Epidemic Etiology and Diagnostics, Guangxi Veterinary Research Institute, 51 Youai North Road, Nanning, 530001, China.
authors.forename: Liji
authors.initials: L
authors.lastname: Xie
authors.affiliation: Department of Biotechnology, Guangxi Key Laboratory of Animal Epidemic Etiology and Diagnostics, Guangxi Veterinary Research Institute, 51 Youai North Road, Nanning, 530001, China. xiezhixun@126.com.
authors.forename: Zhixun
authors.initials: Z
authors.lastname: Xie
authors.affiliation: Department of Biotechnology, Guangxi Key Laboratory of Animal Epidemic Etiology and Diagnostics, Guangxi Veterinary Research Institute, 51 Youai North Road, Nanning, 530001, China.
authors.forename: Li
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Biotechnology, Guangxi Key Laboratory of Animal Epidemic Etiology and Diagnostics, Guangxi Veterinary Research Institute, 51 Youai North Road, Nanning, 530001, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Fan
authors.affiliation: Department of Biotechnology, Guangxi Key Laboratory of Animal Epidemic Etiology and Diagnostics, Guangxi Veterinary Research Institute, 51 Youai North Road, Nanning, 530001, China.
authors.forename: Sisi
authors.initials: S
authors.lastname: Luo
authors.affiliation: Department of Biotechnology, Guangxi Key Laboratory of Animal Epidemic Etiology and Diagnostics, Guangxi Veterinary Research Institute, 51 Youai North Road, Nanning, 530001, China.
authors.forename: Jiaoling
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Biotechnology, Guangxi Key Laboratory of Animal Epidemic Etiology and Diagnostics, Guangxi Veterinary Research Institute, 51 Youai North Road, Nanning, 530001, China.
authors.forename: Xianwen
authors.initials: X
authors.lastname: Deng
authors.affiliation: Department of Biotechnology, Guangxi Key Laboratory of Animal Epidemic Etiology and Diagnostics, Guangxi Veterinary Research Institute, 51 Youai North Road, Nanning, 530001, China.
authors.forename: Zhiqin
authors.initials: Z
authors.lastname: Xie
authors.affiliation: Department of Biotechnology, Guangxi Key Laboratory of Animal Epidemic Etiology and Diagnostics, Guangxi Veterinary Research Institute, 51 Youai North Road, Nanning, 530001, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zeng
authors.affiliation: Department of Biotechnology, Guangxi Key Laboratory of Animal Epidemic Etiology and Diagnostics, Guangxi Veterinary Research Institute, 51 Youai North Road, Nanning, 530001, China.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Biotechnology, Guangxi Key Laboratory of Animal Epidemic Etiology and Diagnostics, Guangxi Veterinary Research Institute, 51 Youai North Road, Nanning, 530001, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Wang
mesh_terms: D000818:Animals; D001715:Bird Diseases / Q000201:enzymology* / Q000235:genetics / Q000821:virology*; D002522:Chlorocebus aethiops; D015967:Gene Expression Regulation, Viral; D054884:Host-Pathogen Interactions; D030101:Orthoreovirus, Avian / Q000235:genetics / Q000378:metabolism*; D058539:Phosphatidylinositol 3-Kinase / Q000235:genetics / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D012088:Reoviridae Infections / Q000201:enzymology* / Q000235:genetics / Q000662:veterinary* / Q000821:virology; D015398:Signal Transduction; D014709:Vero Cells; D014758:Viral Core Proteins / Q000235:genetics / Q000378:metabolism*; D054334:Viral Regulatory and Accessory Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D016601:RNA-Binding Proteins; D014758:Viral Core Proteins; D054334:Viral Regulatory and Accessory Proteins; C031657:sigma NS protein, Reovirus; C403292:sigmaA protein, avian reovirus; D058539:Phosphatidylinositol 3-Kinase; D051057:Proto-Oncogene Proteins c-akt