title: Long intergenic non-coding RNA APOC1P1-3 inhibits apoptosis by decreasing α-tubulin acetylation in breast cancer.
abstract: Increasing evidence indicates that long non-coding RNAs (lncRNAs) act as important regulatory factors in tumor progression. However, their roles in breast cancer remain largely unknown. In present studies, we identified aberrantly expressed long intergenic non-coding RNA APOC1P1-3 (lincRNA-APOC1P1-3) in breast cancer by microarray, verified it by quantitative real-time PCR, and assessed methylation status in the promoter region by pyrosequencing. We also investigated the biological functions with plasmid transfection and siRNA silencing experiments, and further explored their mechanisms by RNA pull-down and RNA immunoprecipitation to identify binding proteins. We found that 224 lncRNAs were upregulated in breast cancer, whereas 324 were downregulated. The lincRNA-APOC1P1-3 was overexpressed in breast cancer, which was related to tumor size and hypomethylation in its promoter region. We also found that APOC1P1-3 could directly bind to tubulin to decrease α-tubulin acetylation, to inactivate caspase-3, and to inhibit apoptosis. This study demonstrates that overexpression of APOC1P1-3 can inhibit breast cancer apoptosis.
authors.affiliation: Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan University, No. 138 Yixueyuan Road, Shanghai, China.
authors.forename: X-H
authors.initials: XH
authors.lastname: Liao
authors.affiliation: Department of Pathology, School of Basic Medical Sciences, Fudan University, No. 138 Yixueyuan Road, Shanghai, China.
authors.forename: J-G
authors.initials: JG
authors.lastname: Wang
authors.affiliation: Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan University, No. 138 Yixueyuan Road, Shanghai, China.
authors.forename: L-Y
authors.initials: LY
authors.lastname: Li
authors.affiliation: Department of Pathology, School of Basic Medical Sciences, Fudan University, No. 138 Yixueyuan Road, Shanghai, China.
authors.forename: D-M
authors.initials: DM
authors.lastname: Zhou
authors.affiliation: Department of Pathology, School of Basic Medical Sciences, Fudan University, No. 138 Yixueyuan Road, Shanghai, China.
authors.forename: K-H
authors.initials: KH
authors.lastname: Ren
authors.affiliation: Department of Breast Surgery, Huashan Hospital, Fudan University, No. 12 Urumqi Middle Road, Shanghai, China.
authors.forename: Y-T
authors.initials: YT
authors.lastname: Jin
authors.affiliation: Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan University, No. 138 Yixueyuan Road, Shanghai, China.
authors.forename: L
authors.initials: L
authors.lastname: Lv
authors.affiliation: Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan University, No. 138 Yixueyuan Road, Shanghai, China.
authors.forename: J-G
authors.initials: JG
authors.lastname: Yu
authors.affiliation: Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan University, No. 138 Yixueyuan Road, Shanghai, China.
authors.forename: J-Y
authors.initials: JY
authors.lastname: Yang
authors.affiliation: Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan University, No. 138 Yixueyuan Road, Shanghai, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Lu
authors.affiliation: Department of Breast Surgery, Huashan Hospital, Fudan University, No. 12 Urumqi Middle Road, Shanghai, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Zou
authors.affiliation: Department of Medicine, University of Louisville, Louisville, KY 40292, USA.
authors.forename: J
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Pathology, School of Basic Medical Sciences, Fudan University, No. 138 Yixueyuan Road, Shanghai, China.|Department of Pathology, The Fifth People's Hospital of Shanghai, Fudan University, No. 128 Ruili Road, Shanghai, China.
authors.forename: X-P
authors.initials: XP
authors.lastname: Liu
authors.affiliation: Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan University, No. 138 Yixueyuan Road, Shanghai, China.
authors.forename: P
authors.initials: P
authors.lastname: Zhou
mesh_terms: D000107:Acetylation; D000328:Adult; D001943:Breast Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019175:DNA Methylation; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D046228:Microarray Analysis; D008875:Middle Aged; D009367:Neoplasm Staging; D011401:Promoter Regions, Genetic; D062085:RNA, Long Noncoding / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014404:Tubulin / Q000235:genetics* / Q000378:metabolism
chemical_list: D062085:RNA, Long Noncoding; D034741:RNA, Small Interfering; D014404:Tubulin; C505407:CASP3 protein, human; D053148:Caspase 3