title: Characterization and Enhanced Antioxidant Activity of the Cysteinyl β-Cyclodextrin-Baicalein Inclusion Complex.
abstract: Baicalein is a type of flavonoid isolated from the roots of a medicinal plant, Scutellaria baicalensis. Although it has attracted considerable attention due to its antiviral, anti-tumor, and anti-inflammatory activities, its limited aqueous solubility inhibits the clinical application of this flavonoid. The present study aimed to prepare and characterize a host-guest complex in an effort to improve the solubility and antioxidant activity of baicalein. The host molecule is a macrocyclic β-cyclodextrin (β-CD) functionalized with cysteine for a synergetic effect. The structure of the synthesized cysteinyl β-CD was analyzed using nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry. The inclusion complex with baicalein was studied by UV-vis, NMR spectroscopy, scanning electron microscopy, and X-ray powder diffractometry. The formed cysteinyl β-CD/baicalein inclusion complex efficiently improved the solubility and antioxidant ability of baicalein. Therefore, we suggest that the present cysteinyl β-CD is a potential host molecule for inclusion complexation and for bioavailability augmentation.
authors.affiliation: Department of Bioscience and Biotechnology, Microbial Carbohydrate Resource Bank (MCRB), Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea. hwanhee@konkuk.ac.kr.
authors.forename: Hwanhee
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Bioscience and Biotechnology, Microbial Carbohydrate Resource Bank (MCRB), Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea. lannyhu0806@hotmail.com.
authors.forename: Hu
authors.initials: H
authors.lastname: Yiluo
authors.affiliation: Department of Applied Chemistry, Dongduk Women's University, Seoul 136-714, Korea. sypark21@dongduk.ac.kr.
authors.forename: Seyeon
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Biological Science, Mokpo National University, Jeonnam 534-729, Korea. jaeyunglee@gmail.com.
authors.forename: Jae Yung
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Center for Biotechnology Research in UBITA (CBRU), Institute for Ubiquitous Information Technology and Applications (UBITA), Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea. echo@konkuk.ac.kr.
authors.forename: Eunae
authors.initials: E
authors.lastname: Cho
authors.affiliation: Department of Bioscience and Biotechnology, Microbial Carbohydrate Resource Bank (MCRB), Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea. shjung@konkuk.ac.kr.|Center for Biotechnology Research in UBITA (CBRU), Institute for Ubiquitous Information Technology and Applications (UBITA), Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea. shjung@konkuk.ac.kr.
authors.forename: Seunho
authors.initials: S
authors.lastname: Jung
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D001682:Biological Availability; D003545:Cysteine / Q000737:chemistry*; D044950:Flavanones / Q000737:chemistry* / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D012995:Solubility; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014961:X-Ray Diffraction; D047392:beta-Cyclodextrins / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D044950:Flavanones; D047392:beta-Cyclodextrins; C006680:baicalein; D003545:Cysteine
keywords: antioxidant activity; baicalein; cysteinyl β-cyclodextrin; inclusion complex