title: Evolution of microbial community along with increasing solid concentration during high-solids anaerobic digestion of sewage sludge.
abstract: High-solids anaerobic digestion (HSAD), a promising method with smaller reactor and less heating energy consumption, showed relatively lower digestion efficiency sometimes and higher tolerance to some inhibitors. To investigate the phenomena, the archaeal and bacterial communities in four anaerobic digesters treating sewage sludge with total solids (TS) of 10-19% were investigated. Although acetoclastic methanogenesis conducted mainly by genus Methanosarcina was still the main pathway producing methane, the total ratio of acetoclastic methanogens decreased along with the increased TS. In contrary, the relative abundance of hydrogenotrophic methanogens increased from 6.8% at TS 10% to 22.3% at TS 19%, and methylotrophic methanogens from 10.4% to 20.9%. The bacterial community was dominated by five phyla. Acidogenic and acetogenic bacteria affiliated to Firmicutes decreased following the increase of TS; while the proteolysis phylum Bacteroidetes increased, with a tolerant family ST-12K33 notably existing in the digesters at TS 17% and 19%.
authors.affiliation: Key Laboratory of Microorganism Application and Risk Control of Shenzhen, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China.
authors.forename: Can
authors.initials: C
authors.lastname: Liu
authors.affiliation: Key Laboratory of Microorganism Application and Risk Control of Shenzhen, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China. Electronic address: li.huan@sz.tsinghua.edu.cn.
authors.forename: Huan
authors.initials: H
authors.lastname: Li
authors.affiliation: Key Laboratory of Microorganism Application and Risk Control of Shenzhen, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China.
authors.forename: Yuyao
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Microorganism Application and Risk Control of Shenzhen, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Si
authors.affiliation: Guangdong Haoyuan Environmental Technology Company, Shenzhen 518055, China.
authors.forename: Qingwu
authors.initials: Q
authors.lastname: Chen
mesh_terms: D000693:Anaerobiosis; D001105:Archaea / Q000378:metabolism*; D001419:Bacteria / Q000378:metabolism*; D044822:Biodiversity; D019149:Bioreactors / Q000382:microbiology; D008697:Methane / Q000378:metabolism; D017020:Methanosarcina / Q000378:metabolism; D010802:Phylogeny; D012037:Refuse Disposal / Q000379:methods*; D012722:Sewage / Q000382:microbiology*
chemical_list: D012722:Sewage; D008697:Methane
keywords: Anaerobic digestion; Microbial community; Sewage sludge; Solid concentration