title: Administration of Myelin Basic Protein Peptides Encapsulated in Mannosylated Liposomes Normalizes Level of Serum TNF-α and IL-2 and Chemoattractants CCL2 and CCL4 in Multiple Sclerosis Patients.
abstract: We have previously shown that immunodominant MBP peptides encapsulated in mannosylated liposomes (Xemys) effectively suppressed experimental allergic encephalomyelitis (EAE). Within the frames of the successfully completed phase I clinical trial, we investigated changes in the serum cytokine profile after Xemys administration in MS patients. We observed a statistically significant decrease of MCP-1/CCL2, MIP-1β/CCL4, IL-7, and IL-2 at the time of study completion. In contrast, the serum levels of TNF-α were remarkably elevated. Our data suggest that the administration of Xemys leads to a normalization of cytokine status in MS patients to values commonly reported for healthy subjects. These data are an important contribution for the upcoming Xemys clinical trials.
authors.affiliation: Institute of Bioorganic Chemistry RAS, Moscow 117997, Russia; Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan 420012, Russia.
authors.forename: Yakov
authors.initials: Y
authors.lastname: Lomakin
authors.affiliation: Institute of Bioorganic Chemistry RAS, Moscow 117997, Russia; Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan 420012, Russia; Institute of Gene Biology RAS, Moscow 119334, Russia.
authors.forename: Alexey
authors.initials: A
authors.lastname: Belogurov
authors.affiliation: Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan 420012, Russia.
authors.forename: Irina
authors.initials: I
authors.lastname: Glagoleva
authors.affiliation: Institute of Bioorganic Chemistry RAS, Moscow 117997, Russia; Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan 420012, Russia.
authors.forename: Alexey
authors.initials: A
authors.lastname: Stepanov
authors.affiliation: OJSC Pharmsynthez, Saint Petersburg 197110, Russia.
authors.forename: Konstantin
authors.initials: K
authors.lastname: Zakharov
authors.affiliation: Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan 420012, Russia.
authors.forename: John
authors.initials: J
authors.lastname: Okunola
authors.affiliation: Institute of Bioorganic Chemistry RAS, Moscow 117997, Russia; Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan 420012, Russia.
authors.forename: Ivan
authors.initials: I
authors.lastname: Smirnov
authors.affiliation: OJSC Pharmsynthez, Saint Petersburg 197110, Russia.
authors.forename: Dmitry
authors.initials: D
authors.lastname: Genkin
authors.affiliation: Institute of Bioorganic Chemistry RAS, Moscow 117997, Russia; Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan 420012, Russia; Chemistry Department, Moscow State University, Moscow 119991, Russia.
authors.forename: Alexander
authors.initials: A
authors.lastname: Gabibov
mesh_terms: D000328:Adult; D000818:Animals; D018932:Chemokine CCL2 / Q000097:blood*; D054407:Chemokine CCL4 / Q000097:blood*; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000097:blood*; D015851:Interleukin-7 / Q000378:metabolism; D008081:Liposomes / Q000737:chemistry*; D008297:Male; D051379:Mice; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000097:blood* / Q000188:drug therapy* / Q000378:metabolism; D004676:Myelin Basic Protein / Q000627:therapeutic use*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*; D055815:Young Adult
chemical_list: C111835:CCL2 protein, human; D018932:Chemokine CCL2; D054407:Chemokine CCL4; D007376:Interleukin-2; D015851:Interleukin-7; D008081:Liposomes; D004676:Myelin Basic Protein; D014409:Tumor Necrosis Factor-alpha