title: Real-Time Imaging of Single HIV-1 Disassembly with Multicolor Viral Particles.
abstract: Viral disassembly is poorly understood and related to the infection mechanism. However, directly observing the process in living cells remains technically challenging. In this study, the genome RNA, capsid, and matrix protein of the HIV-1 virus were labeled with a Ru(II) complex ([Ru(phen)2(dppz)](2+)), the TC-FlAsH/ReAsH system, and EGFP/ECFP, respectively. Using the multicolored virus and single-particle imaging, we were able to track the sequential disassembly process of single HIV-1 virus particles in live host cells. Approximately 0.1% of viral particles were observed to undergo a sequential disassembly process at 60-120 min post infection. The timing and efficiency of the disassembly were influenced by the cellular factor CypA and reverse transcription. The findings facilitate a better understanding of the processes governing the HIV-1 lifecycle. The multicolor labeling protocol developed in this study may find many applications involving virus-host-cell interactions.
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences , Wuhan 430071, PR China.|Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology , Beijing 100029, PR China.
authors.forename: Yingxin
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Sciences, Wuhan University , Wuhan 430072, PR China.
authors.forename: Zhike
authors.initials: Z
authors.lastname: He
authors.affiliation: Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology , Beijing 100029, PR China.
authors.forename: Tianwei
authors.initials: T
authors.lastname: Tan
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences , Wuhan 430071, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences , Wuhan 430071, PR China.
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences , Wuhan 430071, PR China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Song
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences , Wuhan 430071, PR China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences , Wuhan 430071, PR China.
authors.forename: Qinxue
authors.initials: Q
authors.lastname: Hu
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Sciences, Wuhan University , Wuhan 430072, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhou
authors.affiliation: National Center for Biodefense and Infectious Diseases, Department of Molecular and Microbiology, George Mason University , Manassas, Virginia 22030, United States.
authors.forename: Yuntao
authors.initials: Y
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences , Beijing 100101, PR China.
authors.forename: Xian-En
authors.initials: XE
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences , Wuhan 430071, PR China.
authors.forename: Zongqiang
authors.initials: Z
authors.lastname: Cui
keywords: multicolor HIV particles; multiple-stage disassembly; single virus imaging