title: Rhizobium strains differ considerably in outer membrane permeability and polymyxin B resistance.
abstract: Six rhizobium (Rhizobium leguminosarum bv. Trifolii TA1, Sinorhizobium meliloti 1021, Mesorhizobium huakuii IFO 15243(T), Ochrobactrum lupini LUP 21(T), Bradyrhizobium japonicum USDA110 and B. elkanii USDA 76) and two Escherichia coli strains (E. coli ATCC 25922 and E. coli HB 101) were compared in respect to polymyxin B and EDTA resistance, as well as bacterial outer membrane (OM) permeability to a fluorescent hydrophobic agent (N-phenyl-1-naphthylamine - NPN). TEM (Transmission Electron Microscopy) and a microbial test demonstrated that all the rhizobia were much more resistant to polymyxin B in comparison with E. coli strains. EDTA and polymyxin B enhance permeability of B. japonicum and O. lupini OM. Other rhizobia incorporated NPN independently of the presence of membrane-deteriorating agents; however, the level of fluorescence (measured as NPN absorption) was strain dependent.
authors.affiliation: Department of Genetics and Microbiology, Maria Curie-Sklodowska University, Lublin, Poland.
authors.forename: Iwona
authors.initials: I
authors.lastname: Komaniecka
authors.affiliation: Department of Genetics and Microbiology, Maria Curie-Sklodowska University, Lublin, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Zamłyńska
authors.affiliation: Department of Genetics and Microbiology, Maria Curie-Sklodowska University, Lublin, Poland.
authors.forename: Radosław
authors.initials: R
authors.lastname: Zan
authors.affiliation: Department of Biochemistry, Maria Curie-Sklodowska University, Lublin, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Staszczak
authors.affiliation: Department of Comparative Anatomy and Anthropology, Maria Curie-Sklodowska University, Lublin, Poland.
authors.forename: Jarosław
authors.initials: J
authors.lastname: Pawelec
authors.affiliation: Department of Genetics and Microbiology, Maria Curie-Sklodowska University, Lublin, Poland.
authors.forename: Irena
authors.initials: I
authors.lastname: Seta
authors.affiliation: Department of Genetics and Microbiology, Maria Curie-Sklodowska University, Lublin, Poland.
authors.forename: Adam
authors.initials: A
authors.lastname: Choma
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology*; D002462:Cell Membrane / Q000378:metabolism; D002463:Cell Membrane Permeability; D024881:Drug Resistance, Bacterial; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability; D011112:Polymyxin B / Q000378:metabolism / Q000494:pharmacology*; D012231:Rhizobium / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D000900:Anti-Bacterial Agents; D011112:Polymyxin B