title: Orthogonally Functionalizable Polyurethane with Subsequent Modification with Heparin and Endothelium-Inducing Peptide Aiming for Vascular Reconstruction.
abstract: Surface coimmobilization modifications of blood-contacting devices with both antithrombogenic moieties and endothelium-inducing biomolecules may create a synergistic effect to improve their performance. However, it is difficult to perform covalent dual-functionalization with both biomolecules on the surface of normally used synthetic polymeric substrates. Herein, we developed and characterized an orthogonally functionalizable polymer, biodegradable elastic poly(ester urethane)urea with disulfide and amino groups (PUSN), which was further fabricated into electropun fibrous scaffolds and surface modified with heparin and endothelial progenitor cells (EPC) recruiting peptide (TPS). The modification effects were assessed through platelet adhesion, EPC, and HUVEC proliferation. Results showed the dual modified PUSN scaffolds demonstrated a synergistic effect of reduced platelet deposition and improved EPC proliferation in vitro study, and demonstrated their potential application in small diameter vascular regeneration.
authors.affiliation: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University , Shanghai 201620, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Fang
authors.affiliation: Department of Cardiothoracic Surgery, Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine , Shanghai 200127, China.
authors.forename: Jialing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Imaging Diagnosis Center, Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine , Shanghai 200127, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Du
authors.affiliation: Department of Cardiothoracic Surgery, Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine , Shanghai 200127, China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Imaging Diagnosis Center, Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine , Shanghai 200127, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Shi
authors.affiliation: Department of Cardiothoracic Surgery, Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine , Shanghai 200127, China.
authors.forename: Yongxuan
authors.initials: Y
authors.lastname: Peng
authors.affiliation: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University , Shanghai 201620, China.
authors.forename: Weiming
authors.initials: W
authors.lastname: Chen
authors.affiliation: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University , Shanghai 201620, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Yuan
authors.affiliation: McGowan Institute for Regenerative Medicine, University of Pittsburgh , Pittsburgh, Pennsylvania 15219, United States.
authors.forename: Sang-Ho
authors.initials: SH
authors.lastname: Ye
authors.affiliation: McGowan Institute for Regenerative Medicine, University of Pittsburgh , Pittsburgh, Pennsylvania 15219, United States.
authors.forename: William R
authors.initials: WR
authors.lastname: Wagner
authors.affiliation: Department of Cardiothoracic Surgery, Shanghai Children's Medical Center, Shanghai Jiaotong University School of Medicine , Shanghai 200127, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Yin
authors.affiliation: State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University , Shanghai 201620, China.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Mo
mesh_terms: D001807:Blood Vessel Prosthesis; D002448:Cell Adhesion; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004727:Endothelium / Q000166:cytology; D006493:Heparin / Q000378:metabolism; D006801:Humans; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D010973:Platelet Adhesiveness; D011140:Polyurethanes / Q000737:chemistry*; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D020099:Coated Materials, Biocompatible; D010455:Peptides; D011140:Polyurethanes; D006493:Heparin
keywords: endothelialization; orthogonally functionalizable polymer; polyurethane; surface modification; vascular regeneration