title: Deeper insight into the structure of the anaerobic digestion microbial community; the biogas microbiome database is expanded with 157 new genomes.
abstract: This research aimed to better characterize the biogas microbiome by means of high throughput metagenomic sequencing and to elucidate the core microbial consortium existing in biogas reactors independently from the operational conditions. Assembly of shotgun reads followed by an established binning strategy resulted in the highest, up to now, extraction of microbial genomes involved in biogas producing systems. From the 236 extracted genome bins, it was remarkably found that the vast majority of them could only be characterized at high taxonomic levels. This result confirms that the biogas microbiome is comprised by a consortium of unknown species. A comparative analysis between the genome bins of the current study and those extracted from a previous metagenomic assembly demonstrated a similar phylogenetic distribution of the main taxa. Finally, this analysis led to the identification of a subset of common microbes that could be considered as the core essential group in biogas production.
authors.affiliation: Department of Environmental Engineering, Technical University of Denmark, Kgs. Lyngby DK-2800, Denmark.
authors.forename: Laura
authors.initials: L
authors.lastname: Treu
authors.affiliation: Department of Environmental Engineering, Technical University of Denmark, Kgs. Lyngby DK-2800, Denmark. Electronic address: panak@env.dtu.dk.
authors.forename: Panagiotis G
authors.initials: PG
authors.lastname: Kougias
authors.affiliation: Department of Biology, University of Padua, Via U. Bassi 58/b, 35121 Padova, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Campanaro
authors.affiliation: Department of Environmental Engineering, Technical University of Denmark, Kgs. Lyngby DK-2800, Denmark.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Bassani
authors.affiliation: Department of Environmental Engineering, Technical University of Denmark, Kgs. Lyngby DK-2800, Denmark.
authors.forename: Irini
authors.initials: I
authors.lastname: Angelidaki
mesh_terms: D056804:Biofuels; D016208:Databases, Factual; D054892:Metagenome; D056186:Metagenomics / Q000379:methods; D059013:Microbial Consortia; D064307:Microbiota / Q000235:genetics; D010802:Phylogeny
chemical_list: D056804:Biofuels
keywords: Assembly; Binning; Biogas; Metagenome; Microbiome