title: Cell-SELEX-based selection and characterization of a G-quadruplex DNA aptamer against mouse dendritic cells.
abstract: Targeting of dendritic cells (DCs) by aptamers increases antigen capture and presentation to the immune system. Our aim was to produce aptamers against DC molecules using the cell-SELEX procedure. For this purpose, 18 rounds of cell-SELEX were performed on mouse macrophage J774A.1 and CT26 as target and control cells, respectively. The selected aptamers were truncated and their binding to mouse macrophages, and immature and mature DCs analyzed. Two macrophage-specific aptamers, Seq6 and Seq7, were identified. A truncated form of Seq7, Seq7-4, 33 nucleotides in length and containing the G-quadruplex, bound macrophages and immature DCs with KD values in the nanomolar range. We anticipate that Seq7-4 has potential as a therapeutic tool in targeting of mouse macrophages and immature DCs to efficiently improve different immunotherapy approaches.
authors.affiliation: Immunology Research Center, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: MoghadamM1@mums.ac.ir.
authors.forename: M
authors.initials: M
authors.lastname: Moghadam
authors.affiliation: Immunology Research Center, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: SankianM@mums.ac.ir.
authors.forename: M
authors.initials: M
authors.lastname: Sankian
authors.affiliation: Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: abnouskh@mums.ac.ir.
authors.forename: K
authors.initials: K
authors.lastname: Abnous
authors.affiliation: Allergy Research Center, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: varasteha@mums.ac.ir.
authors.forename: A
authors.initials: A
authors.lastname: Varasteh
authors.affiliation: Targeted drug delivery Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: taghdisihm@mums.ac.ir.
authors.forename: S M
authors.initials: SM
authors.lastname: Taghdisi
authors.affiliation: Immunology Research Center, BuAli Research Institute, Department of Immunology and Allergy, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: Mahmoudim@mums.ac.ir.
authors.forename: M
authors.initials: M
authors.lastname: Mahmoudi
authors.affiliation: Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: RamezaniM@mums.ac.ir.
authors.forename: M
authors.initials: M
authors.lastname: Ramezani
authors.affiliation: Immunology Research Center, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: GholizadehZ902@mums.ac.ir.
authors.forename: Z
authors.initials: Z
authors.lastname: Gholizadeh
authors.affiliation: Department of Immunology, Molecular and Medicine Research Center, School of Medicine, Arak University of Medical Sciences, Arak, Iran. Electronic address: aliganjy_1360@yahoo.com.
authors.forename: A
authors.initials: A
authors.lastname: Ganji
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000235:genetics; D052157:Aptamers, Nucleotide / Q000235:genetics*; D045744:Cell Line, Tumor; D019295:Computational Biology; D003713:Dendritic Cells / Q000502:physiology*; D005354:Fibrosarcoma / Q000473:pathology*; D054856:G-Quadruplexes; D006801:Humans; D007167:Immunotherapy / Q000379:methods*; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D052156:SELEX Aptamer Technique
chemical_list: D052157:Aptamers, Nucleotide
keywords: Aptamers; Cell-SELEX; DNA aptamer; G-quadruplex aptamer; J774A.1; Mouse dendritic cell targeting