title: Effects of dietary fibre source on microbiota composition in the large intestine of suckling piglets.
abstract: This study aimed to investigate the effects of dietary fibre sources on the gut microbiota in suckling piglets, and to test the hypothesis that a moderate increase of dietary fibre may affect the gut microbiota during the suckling period. Suckling piglets were fed different fibre-containing diets or a control diet from postnatal day 7 to 22. Digesta samples from cecum, proximal colon and distal colon were used for Pig Intestinal Tract Chip analysis. The data showed that the effects of fibre-containing diet on the gut microbiota differed in the fibre source and gut location. The alfalfa diet increased Clostridium cluster XIVb and Sporobacter termitidis in the cecum compared to the pure cellulose diet. Compared to the control diet, the alfalfa diet also increased Coprococcus eutactus in the distal colon, while the pure cellulose diet decreased Eubacterium pyruvativorans in the cecum. The pure cellulose diet increased Prevotella ruminicola compared to the wheat bran diet. Interestingly, the alfalfa group had the lowest abundance of the potential pathogen Streptococcus suis in the cecum and distal colon. These results indicated that a moderate increase in dietary fibres affected the microbial composition in suckling piglets, and that the alfalfa inclusion produced some beneficial effects on the microbial communities.
authors.affiliation: Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095 Jiangsu, China.
authors.forename: Lingli
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095 Jiangsu, China.
authors.forename: Chunlong
authors.initials: C
authors.lastname: Mu
authors.affiliation: Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095 Jiangsu, China.
authors.forename: Xiangyu
authors.initials: X
authors.lastname: He
authors.affiliation: Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095 Jiangsu, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Su
authors.affiliation: Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095 Jiangsu, China.
authors.forename: Shengyong
authors.initials: S
authors.lastname: Mao
authors.affiliation: Laboratory of Microbiology, Agrotechnology and Food Science Group, Wageningen University, Stippeneng 4. 6708 WE, Wageningen, the Netherlands.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Laboratory of Microbiology, Agrotechnology and Food Science Group, Wageningen University, Stippeneng 4. 6708 WE, Wageningen, the Netherlands.
authors.forename: Hauke
authors.initials: H
authors.lastname: Smidt
authors.affiliation: Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095 Jiangsu, China zhuweiyun@njau.edu.cn.
authors.forename: Weiyun
authors.initials: W
authors.lastname: Zhu
mesh_terms: D000821:Animal Feed; D000818:Animals; D000831:Animals, Newborn; D044822:Biodiversity; D016000:Cluster Analysis; D004043:Dietary Fiber; D000069196:Gastrointestinal Microbiome; D007420:Intestine, Large / Q000382:microbiology*; D054892:Metagenome; D056186:Metagenomics / Q000379:methods; D013552:Swine
keywords: fibre; large intestine; microbial communities; suckling piglets