title: Improved operational stability of d-psicose 3-epimerase by a novel protein engineering strategy, and d-psicose production from fruit and vegetable residues.
abstract: The aim of the present work was to improve stability of d-psicose 3-epimerase and biotransformation of fruit and vegetable residues for d-psicose production. The study established that N-terminal fusion of a yeast homolog of SUMO protein - Smt3 - can confer elevated optimal temperature and improved operational stability to d-psicose 3-epimerase. The Smt3-d-psicose 3-epimerase conjugate system exhibited relatively better catalytic efficiency, and improved productivity in terms of space-time yields of about 8.5kgL(-1)day(-1). It could serve as a promising catalytic tool for the pilot scale production of the functional sugar, d-psicose. Furthermore, a novel approach for economical production of d-psicose was developed by enzymatic and microbial bioprocessing of fruit and vegetable residues, aimed at epimerization of in situd-fructose to d-psicose. The bioprocessing led to achievement of d-psicose production to the extent of 25-35% conversion (w/w) of d-fructose contained in the sample.
authors.affiliation: Center of Innovative and Applied Bioprocessing (CIAB), Department of Biotechnology (DBT), Mohali, India.
authors.forename: Satya Narayan
authors.initials: SN
authors.lastname: Patel
authors.affiliation: Center of Innovative and Applied Bioprocessing (CIAB), Department of Biotechnology (DBT), Mohali, India.
authors.forename: Manisha
authors.initials: M
authors.lastname: Sharma
authors.affiliation: Center of Innovative and Applied Bioprocessing (CIAB), Department of Biotechnology (DBT), Mohali, India.
authors.forename: Kusum
authors.initials: K
authors.lastname: Lata
authors.affiliation: Center of Innovative and Applied Bioprocessing (CIAB), Department of Biotechnology (DBT), Mohali, India.
authors.forename: Umesh
authors.initials: U
authors.lastname: Singh
authors.affiliation: Center of Innovative and Applied Bioprocessing (CIAB), Department of Biotechnology (DBT), Mohali, India.
authors.forename: Vinod
authors.initials: V
authors.lastname: Kumar
authors.affiliation: Center of Innovative and Applied Bioprocessing (CIAB), Department of Biotechnology (DBT), Mohali, India.
authors.forename: Rajender S
authors.initials: RS
authors.lastname: Sangwan
authors.affiliation: Center of Innovative and Applied Bioprocessing (CIAB), Department of Biotechnology (DBT), Mohali, India. Electronic address: pratapsudhir17@gmail.com.
authors.forename: Sudhir P
authors.initials: SP
authors.lastname: Singh
mesh_terms: D002384:Catalysis; D003001:Cloning, Molecular; D005632:Fructose / Q000378:metabolism*; D005638:Fruit / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007477:Ions; D007700:Kinetics; D008670:Metals / Q000494:pharmacology; D008970:Molecular Weight; D015202:Protein Engineering / Q000379:methods*; D021122:Protein Subunits / Q000378:metabolism; D019998:Racemases and Epimerases / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000302:isolation & purification; D013696:Temperature; D014675:Vegetables / Q000737:chemistry*
chemical_list: D007477:Ions; D008670:Metals; D021122:Protein Subunits; D011994:Recombinant Proteins; C003243:psicose; D005632:Fructose; D019998:Racemases and Epimerases
keywords: Bioprocessing; Fruit and vegetable waste; Residual biomass; Smt3; d-Psicose; d-Psicose 3-epimerase