title: Peroxisome proliferator-activated receptors (PPARα, PPARγ and PPARβ/δ) gene expression profile on ram spermatozoa and their relation to the sperm motility.
abstract: Peroxisome proliferator-activated receptors (PPARs) are a member of nuclear receptors superfamily, which mainly regulate the expression of target genes involved in lipid and energy metabolism. These receptors are divided to three isotypes: PPARα, PPARγ and PPARβ/δ. Each isotype has a distinct tissue distribution relating to the distinct functions. In this study, the mRNA abundance for PPARα, PPARγ and PPARβ/δ was evaluated and compared with high and low motile ram spermatozoa. Semen samples from 6 adult rams were fractionated on a two layer discontinuous Percoll gradient to high and low motile sperm and quantitative parameters of sperm motility were determined by CASA. Total RNA was extracted and the mRNA abundance for each gene was measured by relative quantification technique with Real time PCR. The levels of three isotypes of PPAR transcripts were significantly higher in high motile semen samples using quantitative RT-PCR. Some of sperm motility indices were also significantly correlated with PPARα and PPARγ relative expression. This study revealed the novel association of PPAR gene isotypes with sperm motility. Data from our study suggested PPARs are one of the possible factors that can be studied in male infertility.
authors.affiliation: Department of Clinical Science, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran; ; Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran;
authors.forename: Ali
authors.initials: A
authors.lastname: Kadivar
authors.affiliation: Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran;; Students' Research Committee, Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran;
authors.forename: Heidar
authors.initials: H
authors.lastname: Heidari Khoei
authors.affiliation: Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran;
authors.forename: Hossein
authors.initials: H
authors.lastname: Hassanpour
authors.affiliation: Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran;
authors.forename: Hamid
authors.initials: H
authors.lastname: Ghanaei
authors.affiliation: Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran;
authors.forename: Arefeh
authors.initials: A
authors.lastname: Golestanfar
authors.affiliation: Department of Animal Science, Faculty of Agriculture, Shahrekord University, Shahrekord, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Mehraban
authors.affiliation: Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran;
authors.forename: Najmeh
authors.initials: N
authors.lastname: Davoodian
authors.affiliation: Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran;
authors.forename: Roohollah
authors.initials: R
authors.lastname: Dehghani Tafti
keywords: Gene expression; Peroxisome proliferator-activated receptors; Ram; Sperm motility