title: New arrangements on several species subcomplexes of Triatoma genus based on the chromosomal position of ribosomal genes (Hemiptera - Triatominae).
abstract: The hemipteran subfamily Triatominae includes 150 blood-sucking species, vectors of Chagas disease. By far the most specious genus is Triatoma, assembled in groups, complexes and subcomplexes based on morphological similarities, geographic distribution and genetic data. However, many molecular studies questioned the species integration of several subcomplexes as monophyletic units. In triatomines, chromosomal position of major ribosomal DNA (rDNA) loci is extremely variable but seems to be species-specific and an evolutionary conserved genetic trait, so that closely related species tend to have ribosomal clusters in the same chromosomal location. Considering that the autosomal position as the ancestral character for all heteropteran species, including triatomines, we suggest that the movement of rDNA loci from autosomes to sex chromosomes rapidly established reproductive barriers between divergent lineages. We proposed that the rDNA translocation from the autosomes to the sex chromosomes restrict reproductive compatibility and eventually promote speciation processes. We analyzed the chromosomal position of 45S rDNA clusters in almost all species of the matogrossensis, rubrovaria, maculata and sordida subcomplexes. The fluorescent in situ hybridization results are discussed considering the available genetic data and we proposed new arrangements in the species that constitute each one of these subcomplexes.
authors.affiliation: Sección Genética Evolutiva, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay.
authors.forename: Sebastián
authors.initials: S
authors.lastname: Pita
authors.affiliation: Departamento de Biología Experimental, Área de Genética, Universidad de Jaén, Jaén, Spain.
authors.forename: Pedro
authors.initials: P
authors.lastname: Lorite
authors.affiliation: Cátedra de Introducción a la Biología, Facultad de Ciencias Exactas Físicas y Naturales, Instituto de Investigaciones Biológicas y Tecnológicas (IIByT), Universidad Nacional de Córdoba (UNC-CONICET), Córdoba, Argentina.
authors.forename: Julieta
authors.initials: J
authors.lastname: Nattero
authors.affiliation: Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos, Instituto Oswaldo Cruz - FIOCRUZ, Rio de Janeiro, Brazil.
authors.forename: Cleber
authors.initials: C
authors.lastname: Galvão
authors.affiliation: Laboratorio de Biologia Celular, Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista "Júlio de Mesquita Filho" (IBILCE-UNESP), São José do Rio Preto, São Paulo, Brazil.
authors.forename: Kaio C C
authors.initials: KC
authors.lastname: Alevi
authors.affiliation: Laboratório Interdisciplinar de Vigilância Entomológica em Diptera e Hemiptera (LIVEDIH), Instituto Oswaldo Cruz/Fundação Oswaldo Cruz, Rio de Janeiro, Brazil; Programa de Pós-Graduação em Biologia Animal, Universidade Federal Rural do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Simone C
authors.initials: SC
authors.lastname: Teves
authors.affiliation: Laboratorio de Biologia Celular, Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista "Júlio de Mesquita Filho" (IBILCE-UNESP), São José do Rio Preto, São Paulo, Brazil.
authors.forename: Maria T V
authors.initials: MT
authors.lastname: Azeredo-Oliveira
authors.affiliation: Sección Genética Evolutiva, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay. Electronic address: fcopanzera@gmail.com.
authors.forename: Francisco
authors.initials: F
authors.lastname: Panzera
mesh_terms: D000349:Africa; D000818:Animals; D059006:Chromosomes, Insect / Q000737:chemistry*; D004275:DNA, Ribosomal / Q000235:genetics*; D004199:Disease Vectors; D005260:Female; D056426:Genetic Loci; D049810:Genetic Speciation; D017404:In Situ Hybridization, Fluorescence; D007843:Latin America; D008297:Male; D005810:Multigene Family; D010802:Phylogeny; D058974:Phylogeography; D012098:Reproduction / Q000235:genetics; D013045:Species Specificity; D014225:Triatominae / Q000145:classification / Q000235:genetics*
chemical_list: D004275:DNA, Ribosomal
keywords: Chagas disease vectors; FISH; Holocentric chromosomes; Location changes of rDNA clusters; Triatominae