title: Antiviral anthraquinones and azaphilones produced by an endophytic fungus Nigrospora sp. from Aconitum carmichaeli.
abstract: A new hydroanthraquinone derivative, 6-O-demethyl-4-dehydroxyaltersolanol A (1), and two new azaphilones, 8,11-didehydrochermesinone B (6) and (7S)-7-hydroxy-3,7-dimethyl-isochromene-6,8-dione (8), along with five known analogues (2-5 and 7), were isolated from the culture broth of Nigrospora sp. YE3033, an endophytic fungus obtained from Aconitum carmichaeli. Their structures were elucidated on the basis of spectroscopic analyses. Biological activity test indicated that compounds 1-3, and 7 exhibited the inhibitory effects on influenza viral strain of A/Puerto Rico/8/34 (H1N1) with the IC50 values of 2.59, 8.35, 7.82, and 0.80μg/mL, respectively, while the low cytotoxicity of 7 with the CC50 value of 184.75μg/mL, displaying a promising potential of 7 in the development of anti-influenza A virus drugs.
authors.affiliation: Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors.forename: Shou-Peng
authors.initials: SP
authors.lastname: Zhang
authors.affiliation: School of Chemical Science and Technology, Yunnan University, Kunming 650091, PR China.
authors.forename: Rong
authors.initials: R
authors.lastname: Huang
authors.affiliation: Group of Peptides and Natural Products Research, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, PR China.
authors.forename: Fang-Fang
authors.initials: FF
authors.lastname: Li
authors.affiliation: Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors.forename: Hong-Xia
authors.initials: HX
authors.lastname: Wei
authors.affiliation: Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors.forename: Xiao-Wei
authors.initials: XW
authors.lastname: Fang
authors.affiliation: Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China.
authors.forename: Xiao-Song
authors.initials: XS
authors.lastname: Xie
authors.affiliation: Group of Peptides and Natural Products Research, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, PR China.
authors.forename: Dong-Guo
authors.initials: DG
authors.lastname: Lin
authors.affiliation: Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, PR China. Electronic address: shwu123@126.com.
authors.forename: Shao-Hua
authors.initials: SH
authors.lastname: Wu
authors.affiliation: Group of Peptides and Natural Products Research, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, PR China. Electronic address: jianhe@smu.edu.cn.
authors.forename: Jian
authors.initials: J
authors.lastname: He
mesh_terms: D031841:Aconitum / Q000382:microbiology*; D000818:Animals; D000880:Anthraquinones / Q000737:chemistry* / Q000302:isolation & purification; D000998:Antiviral Agents / Q000737:chemistry* / Q000302:isolation & purification; D001203:Ascomycota / Q000737:chemistry*; D001578:Benzopyrans / Q000737:chemistry* / Q000302:isolation & purification; D004285:Dogs; D060026:Endophytes / Q000737:chemistry; D053118:Influenza A Virus, H1N1 Subtype / Q000187:drug effects*; D061985:Madin Darby Canine Kidney Cells; D015394:Molecular Structure; D010860:Pigments, Biological / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: C000614006:6-O-demethyl-4-dehydroxyaltersolanol A; D000880:Anthraquinones; D000998:Antiviral Agents; D001578:Benzopyrans; D010860:Pigments, Biological; C494154:azaphilone
keywords: Anthraquinone; Anti-influenza activity; Azaphilone; Endophytic fungus; Nigrospora sp.