title: A Versatile Multiple Target Detection System Based on DNA Nano-assembled Linear FRET Arrays.
abstract: DNA molecules have been utilized both as powerful synthetic building blocks to create nanoscale architectures and as inconstant programmable templates for assembly of biosensors. In this paper, a versatile, scalable and multiplex detection system is reported based on an extending fluorescent resonance energy transfer (FRET) cascades on a linear DNA assemblies. Seven combinations of three kinds of targets are successfully detected through the changes of fluorescence spectra because of the three-steps FRET or non-FRET continuity mechanisms. This nano-assembled FRET-based nanowire is extremely significant for the development of rapid, simple and sensitive detection system. The method used here could be extended to a general platform for multiplex detection through more-step FRET process.
authors.affiliation: School of Chemistry &Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
authors.forename: Yansheng
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Chemistry &Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
authors.forename: Hongwu
authors.initials: H
authors.lastname: Du
authors.affiliation: School of Chemistry &Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
authors.forename: Wenqian
authors.initials: W
authors.lastname: Wang
authors.affiliation: Peking University People's Hospital, Beijing, 100044, China.
authors.forename: Peixun
authors.initials: P
authors.lastname: Zhang
authors.affiliation: School of Chemistry &Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Xu
authors.affiliation: School of Chemistry &Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Wen
authors.affiliation: School of Chemistry &Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
authors.forename: Xueji
authors.initials: X
authors.lastname: Zhang
mesh_terms: D020029:Base Pairing; D015374:Biosensing Techniques; D004247:DNA / Q000032:analysis*; D004277:DNA, Single-Stranded / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry; D053770:Nanowires; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods; D017785:Photons; D012996:Solutions; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D004277:DNA, Single-Stranded; D005456:Fluorescent Dyes; D012996:Solutions; D004247:DNA