title: Detecting Reactive Oxygen Species Generation and Stem Cell Proliferation in the Drosophila Intestine.
abstract: The conservation of intestinal stem cell crypt dynamics between Drosophila melanogaster and mammals allows for the genetically tractable fly model to be used for analyses of intestinal development, homeostasis, and renewal in relation to microbiota. The invertebrate fly model is advantageous for genetic research due to its anatomical and genetic simplicity and short lifespan. Accordingly, experimental resources such as large numbers of mutant and genetically modified flies have been developed. We have developed techniques to generate germ-free Drosophila, monoassociate them with candidate bacteria, and assess ensuing physiological responses within the gut tissue that include the generation of reactive oxygen species and cell proliferation.
authors.affiliation: Department of Pediatrics, Emory University School of Medicine, Whitehead Building, Room 105F, 615 Michael Street, Atlanta, GA, 30322, USA.
authors.forename: Liping
authors.initials: L
authors.lastname: Luo
authors.affiliation: Department of Pathology, Emory University School of Medicine, Atlanta, GA, 30322, USA.
authors.forename: April R
authors.initials: AR
authors.lastname: Reedy
authors.affiliation: Department of Pediatrics, Emory University School of Medicine, Whitehead Building, Room 105F, 615 Michael Street, Atlanta, GA, 30322, USA. rjones5@emory.edu.
authors.forename: Rheinallt M
authors.initials: RM
authors.lastname: Jones
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D049109:Cell Proliferation; D000066673:Cell Self Renewal; D004331:Drosophila melanogaster / Q000166:cytology / Q000378:metabolism / Q000382:microbiology*; D005854:Germ Cells / Q000378:metabolism / Q000382:microbiology*; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000166:cytology / Q000382:microbiology*; D048191:Lactiplantibacillus plantarum / Q000502:physiology; D017382:Reactive Oxygen Species / Q000032:analysis*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species
keywords: Cell proliferation; Commensal bacteria; Drosophila melanogaster; Germ-free; Monoassociation; Probiotics; ROS; Reactive oxygen species; Symbiosis