title: MiRNA-211 suppresses cell proliferation, migration and invasion by targeting SPARC in human hepatocellular carcinoma.
abstract: Previous studies have shown that the expression of miR-211 was downregulated in hepatocellular carcinoma (HCC). However, the molecular function and mechanism of miR-211 in HCC growth and invasion are largely unclear. We found that miR-211 is downregulated in HCC tissues and cell lines, respectively. Further results showed that low miR-211 associated with TNM stage, vein invasion status, and poor prognosis. Ectopic expression of miR-211 effectively suppressed HCC cell proliferation, migration and invasion both in vitro and in vivo. We identified SPARC as a bona fide target of miR-211, and overexpression of miR-211 decreased the mRNA and protein expression of SPARC. Finally, we confirmed that the overexpression of SPARC in miR-211-expressing HCC cells can partially restore the inhibitory effect of miR-211. Taken together, our results demonstrated that loss of miR-211 expression and thus uncontrolled SPARC overexpression might drive progression of HCC, which may provide a novel therapeutic strategy for the treatment of HCC.
authors.affiliation: Department of General Surgery, Shanghai First People's Hospital, Shanghai Jiao Tong University, 100 Haining Road, Shanghai, 200080, China.
authors.forename: Biao
authors.initials: B
authors.lastname: Deng
authors.affiliation: Department of General Surgery, Shanghai First People's Hospital, Shanghai Jiao Tong University, 100 Haining Road, Shanghai, 200080, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Qu
authors.affiliation: Department of Neurology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
authors.forename: Jinfang
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Gastroenterology, Tianyou Hospital, TongJi University, 500 Zhennan Road, Shanghai, 200331, China.
authors.forename: Jiaqing
authors.initials: J
authors.lastname: Fang
authors.affiliation: Department of General Surgery, Shanghai First People's Hospital, Shanghai Jiao Tong University, 100 Haining Road, Shanghai, 200080, China.
authors.forename: Shouwen
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Gastroenterology, Shanghai First People's Hospital, Shanghai Jiao Tong University, 100 Haining Road, Shanghai, 200080, China.
authors.forename: Zhongwei
authors.initials: Z
authors.lastname: Cao
authors.affiliation: Department of Gastroenterology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
authors.forename: Zhechuan
authors.initials: Z
authors.lastname: Mei
authors.affiliation: Department of General Surgery, Shanghai First People's Hospital, Shanghai Jiao Tong University, 100 Haining Road, Shanghai, 200080, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Sun
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002465:Cell Movement; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D057809:HEK293 Cells; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D015676:Osteonectin / Q000096:biosynthesis* / Q000235:genetics; D012334:RNA, Neoplasm / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C549405:MIRN211 microRNA, human; D035683:MicroRNAs; D009363:Neoplasm Proteins; D015676:Osteonectin; D012334:RNA, Neoplasm; C577241:SPARC protein, human