title: Protocols for Cloning, Expression, and Functional Analysis of Sirtuin2 (SIRT2).
abstract: SIRT2 is a NAD(+)-dependent deacetylase that belongs to the sirtuin family, which is comprised of seven members (SIRT1-SIRT7) in humans. Furthermore, recent study shows that the Sirt2 gene has three transcript variants in mice. Several diverse proteins have been identified as SIRT2 substrates. SIRT2 activity involves multiple cell processes including growth, differentiation, and energy metabolism. However, little is known of SIRT2's role in oligodendrocytes or in the myelin sheath, where it is an important component. Here we describe procedures that detail Sirt2 gene cloning, identification, expression, and biological analysis in cultured cells.
authors.affiliation: Laboratory of Molecular Cell Biology, College of Pharmacy and Nutrition and Neuroscience Research Cluster, University of Saskatchewan, Room 215-110 Science Place, Thorvaldson Building, Saskatoon, S7N 5C9, Canada. jishaoping@hotmail.com.|Department of Molecular Biology and Biochemistry, Medical School, Henan University, Henan, China. jishaoping@hotmail.com.
authors.forename: Shaoping
authors.initials: S
authors.lastname: Ji
authors.affiliation: Department of Anatomy and Cell Biology, College of Medicine, University of Saskatchewan, Saskatoon, S7N 5C9, Canada.
authors.forename: J Ronald
authors.initials: JR
authors.lastname: Doucette
authors.affiliation: Laboratory of Molecular Cell Biology, College of Pharmacy and Nutrition and Neuroscience Research Cluster, University of Saskatchewan, Room 215-110 Science Place, Thorvaldson Building, Saskatoon, S7N 5C9, Canada. aj.nazarali@usask.ca.
authors.forename: Adil J
authors.initials: AJ
authors.lastname: Nazarali
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D003001:Cloning, Molecular / Q000379:methods*; D015870:Gene Expression; D057809:HEK293 Cells; D006801:Humans; D009186:Myelin Sheath / Q000378:metabolism; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism; D011485:Protein Binding; D051381:Rats; D056565:Sirtuin 2 / Q000235:genetics* / Q000378:metabolism*
chemical_list: C097475:Sirt2 protein, rat; D056565:Sirtuin 2
keywords: Cloning; Expression; Knockdown; SIRT2; Sirtuins; cDNA