title: Assay of NAPE-PLD Activity.
abstract: N-acyl-phosphatidylethanolamine (NAPE)-hydrolyzing phospholipase D (NAPE-PLD) is a prominent enzyme involved in the biosynthesis of fatty acid amides (FAAs), a family of bioactive lipids including anandamide (AEA) as the prototypical member. Here, we describe a NAPE-PLD assay based on radioactive substrates and product separation by thin-layer chromatography (TLC).
authors.affiliation: Department of Experimental Medicine and Surgery, Tor Vergata University of Rome, Rome, Italy. filomena.fezza@uniroma2.it.
authors.forename: Filomena
authors.initials: F
authors.lastname: Fezza
authors.affiliation: Center of Integrated Research, Campus Bio-Medico University of Rome, Rome, Italy.
authors.forename: Nicolina
authors.initials: N
authors.lastname: Mastrangelo
authors.affiliation: Center of Integrated Research, Campus Bio-Medico University of Rome, Rome, Italy.|European Center for Brain Research/IRCCS Santa Lucia Foundation, Rome, Italy.
authors.forename: Mauro
authors.initials: M
authors.lastname: Maccarrone
mesh_terms: D002460:Cell Line; D002855:Chromatography, Thin Layer; D004789:Enzyme Activation; D057075:Enzyme Assays; D007553:Isotope Labeling; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D010739:Phospholipase D / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D010714:Phosphatidylethanolamines; D010739:Phospholipase D
keywords: Anandamide; Fatty acid amides; N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D; N-acyl-phosphatidylethanolamines; N-palmitoylethanolamine; Radioactive assay; Thin-layer chromatography