title: Schwann cell differentiation inhibits interferon-gamma induction of expression of major histocompatibility complex class II and intercellular adhesion molecule-1.
abstract: Interferon-gamma (IFN-γ) upregulates major histocompatibility complex class II (MHC class II) antigens and intercellular adhesion molecule-1 (ICAM-1) on Schwann cells (SC) in vitro, but in nerves of animals and patients MHC class II is primarily expressed on inflammatory cells. We investigated whether SC maturation influences their expression. IFN-γ induced MHC class II and upregulated ICAM-1; the axolemma-like signal 8-bromo cyclic adenosine monophosphate (8 Br cAMP) with IFN-γ inhibited expression. Delaying addition of 8 Br cAMP to SC already exposed to IFN-γ inhibited ongoing expression; addition of IFN-γ to SC already exposed to 8 Br cAMP resulted in minimal expression. Variability of cytokine-induced MHC class II and ICAM-1 expression by SC in vivo may represent the variability of signals from axolemma.
authors.affiliation: Department of Neurology, Wayne State University School of Medicine, 4201 St. Antoine Blvd., Detroit, MI 48201, USA; Department of Immunology and Microbiology, Wayne State University School of Medicine, 4201 St. Antoine Blvd., Detroit, MI 48201, USA. Electronic address: rlisak@med.wayne.edu.
authors.forename: Robert P
authors.initials: RP
authors.lastname: Lisak
authors.affiliation: Department of Neurology, Wayne State University School of Medicine, 4201 St. Antoine Blvd., Detroit, MI 48201, USA.
authors.forename: Beverly
authors.initials: B
authors.lastname: Bealmear
authors.affiliation: Department of Neurology, Wayne State University School of Medicine, 4201 St. Antoine Blvd., Detroit, MI 48201, USA; Department of Immunology and Microbiology, Wayne State University School of Medicine, 4201 St. Antoine Blvd., Detroit, MI 48201, USA.
authors.forename: Joyce A
authors.initials: JA
authors.lastname: Benjamins
mesh_terms: D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D005786:Gene Expression Regulation / Q000187:drug effects*; D000949:Histocompatibility Antigens Class II / Q000378:metabolism*; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D007371:Interferon-gamma / Q000494:pharmacology*; D051381:Rats; D012583:Schwann Cells / Q000187:drug effects*; D013997:Time Factors
chemical_list: D000949:Histocompatibility Antigens Class II; D018799:Intercellular Adhesion Molecule-1; D015124:8-Bromo Cyclic Adenosine Monophosphate; D007371:Interferon-gamma
keywords: Axolemma; Cytokines; Demyelinating neuropathy; Interferon; Peripheral nervous system; Schwann cells