title: A high-molecular-weight, alkaline, and thermostable β-1,4-xylanase of a subseafloor Microcella alkaliphila.
abstract: An endo β-1,4-xylanase (XynE15) from a culture broth of a deep subseafloor microorganism, Microcella alkaliphila JAM-AC0309, was purified to homogeneity. The molecular mass of XynE15 was approximately 150 kDa as judged by SDS-PAGE. The optimal pH and temperature for hydrolysis of xylan were pH 8 and 65 °C. The enzyme was stable to incubation for 30 min at up to 75 °C, and the half-life at 50 °C was 48 h. XynE15 hydrolyzed arabinoxylan, oat spelt xylan, and birchwood xylan well, but not avicel, carboxymethylcellulose, or arabinan. Xylooligosaccharides were hydrolyzed to mainly xylobiose from higher than xylotetraose. The genome sequencing analysis of strain JAM-AC03039 revealed that XynE15 was composed of 1,319 amino acids with one catalytic domain and three carbohydrate-binding domains belonging to glycoside hydrolase (GH) family 10 and carbohydrate-binding module (CBM) family 4, respectively.
authors.affiliation: Graduate School of Engineering, Toyo University, 2100 Kujirai, Kawagoe, Saitama, 350-8585, Japan.|Yupo Corporation, Kamisu, Ibaraki, 314-0102, Japan.
authors.forename: Koki
authors.initials: K
authors.lastname: Kuramochi
authors.affiliation: Research & Development Center for Marine Biosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima, Yokosuka, Kanagawa, 237-0061, Japan.
authors.forename: Kohsuke
authors.initials: K
authors.lastname: Uchimura
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agriculture, Kinki University, 3327-204 Nakamachi, Nara City, Nara, 631-8505, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kurata
authors.affiliation: Research & Development Center for Marine Biosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima, Yokosuka, Kanagawa, 237-0061, Japan. kobayashit@jamstec.go.jp.
authors.forename: Tohru
authors.initials: T
authors.lastname: Kobayashi
authors.affiliation: Electronics Inspired-Interdisciplinary Research Institute, Toyohashi University of Technology, 1-1 Hibarigaoka, Tempaku, Toyohashi, Aichi, 441-8580, Japan.
authors.forename: Yuu
authors.initials: Y
authors.lastname: Hirose
authors.affiliation: Graduate School of Life Science, Toyo University, 1-1-1 Izumino, Itakura, Oura-gun, Gunma, 374-0193, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Miura
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agriculture, Kinki University, 3327-204 Nakamachi, Nara City, Nara, 631-8505, Japan.
authors.forename: Noriaki
authors.initials: N
authors.lastname: Kishimoto
authors.affiliation: Graduate School of Engineering, Toyo University, 2100 Kujirai, Kawagoe, Saitama, 350-8585, Japan.
authors.forename: Ron
authors.initials: R
authors.lastname: Usami
authors.affiliation: Research & Development Center for Marine Biosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima, Yokosuka, Kanagawa, 237-0061, Japan.
authors.forename: Koki
authors.initials: K
authors.lastname: Horikoshi
mesh_terms: D000192:Actinomycetales / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020134:Catalytic Domain; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004795:Enzyme Stability; D016680:Genome, Bacterial; D019015:Geologic Sediments / Q000382:microbiology; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D043364:Endo-1,4-beta Xylanases
keywords: Alkaline; Chikyu; Deep subseafloor; Microcella alkaliphila; Thermostable; Xylanase