title: First characterization of three cyclophilin family proteins in the oyster, Crassostrea ariakensis Gould.
abstract: Cyclophilins (CyPs) are a family of proteins that bind the immunosuppressive agent cyclosporin A (CsA) with high-affinity and belong to one of the three superfamilies of peptidyl-prolyl cis-trans isomerases (PPIase). In this report, three cyclophilin genes (Ca-CyPs), including Ca-CyPA, Ca-CyPB and Ca-PPIL3, were identified from oyster, Crassostrea ariakensis Gould in which Ca-CyPA encodes a protein with 165 amino acid sequences, Ca-CyPB encodes a protein with 217 amino acid sequences and Ca-PPIL3 encodes a protein with 162 amino acid sequences. All of the three Ca-CyPs genes contain a typical CyP-PPIase domain with its signature sequences and Ca-CyPB contains an N-signal peptide sequences. Tissue distribution study revealed that Ca-CyPs were ubiquitously expressed in all examined tissues and the highest levels were observed in hemocytes. RLO incubation upregulated the mRNA expression levels of Ca-CyPs, indicating that three Ca-CyPs might be involved in oyster immune response against RLO infection.
authors.affiliation: Laboratory of Marine Life Science and Technology, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, China; School of Life Sciences, Shaoxing University, Shaoxing, Zhejiang, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Xu
authors.affiliation: Laboratory of Marine Life Science and Technology, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Jiasong
authors.initials: J
authors.lastname: Xie
authors.affiliation: School of Life Sciences, Shaoxing University, Shaoxing, Zhejiang, China.
authors.forename: Shoubao
authors.initials: S
authors.lastname: Yang
authors.affiliation: Laboratory of Marine Life Science and Technology, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Shigen
authors.initials: S
authors.lastname: Ye
authors.affiliation: Laboratory of Marine Life Science and Technology, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Luo
authors.affiliation: Laboratory of Marine Life Science and Technology, College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, China; Ocean College, Qinzhou University, Qinzhou City, Guangxi, China. Electronic address: wuxz@zju.edu.cn.
authors.forename: Xinzhong
authors.initials: X
authors.lastname: Wu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D049896:Crassostrea / Q000235:genetics* / Q000276:immunology*; D021983:Cyclophilins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D007113:Immunity, Innate; D021382:Protein Sorting Signals; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment / Q000662:veterinary
chemical_list: D018076:DNA, Complementary; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D021983:Cyclophilins
keywords: Ca-CyPs; Crassostrea ariakensis; Cyclophilin; Immune response; Rickettsia-like organism