title: Glucosamines Attenuate Bone Loss Due to Menopause by Regulating Osteoclast Function in Ovariectomized Mice.
abstract: The effect of glucosamine (GlcN) and N-acetylglucosamine (GlcNAc) on bone metabolism in ovariectomized (OVX) mice was studied. After 12 weeks of feeding with 0.2% GlcN and 0.2% GlcNAc, the femoral bone mineral density in OVX mice was significantly increased compared with that in OVX mice fed the control diet. Histomorphometric analysis of the tibia indicated that the rates of osteogenesis and bone resorption were reduced due to the GlcN diet. The erosion depth of osteoclasts on the tibia in GlcN- and GlcNAc-fed OVX mice was significantly lower than that in the control OVX mice. The number of tartrate-resistant acid phosphatase-positive osteoclasts induced from bone marrow stem cells isolated from GlcN-fed OVX mice was significantly lower than that from control OVX mice. A loss of uterine weight and higher serum calcium concentration in the GlcN- and GlcNAc-fed OVX mice were observed. The results suggest that the intake of GlcN suppresses bone loss by inhibiting osteoclast differentiation and activity in a nonestrogenic manner.
authors.affiliation: Faculty of Pharmaceutical Sciences, Josai University.
authors.forename: Hironobu
authors.initials: H
authors.lastname: Asai
authors.forename: Sachie
authors.initials: S
authors.lastname: Nakatani
authors.forename: Takuya
authors.initials: T
authors.lastname: Kato
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Shimizu
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mano
authors.forename: Kenji
authors.initials: K
authors.lastname: Kobata
authors.forename: Masahiro
authors.initials: M
authors.lastname: Wada
mesh_terms: D000818:Animals; D015519:Bone Density / Q000187:drug effects; D001854:Bone Marrow Cells / Q000166:cytology; D001862:Bone Resorption / Q000097:blood / Q000188:drug therapy* / Q000378:metabolism; D001842:Bone and Bones / Q000187:drug effects / Q000378:metabolism; D002118:Calcium / Q000097:blood; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D005944:Glucosamine / Q000494:pharmacology / Q000627:therapeutic use*; D008274:Magnesium / Q000097:blood; D008593:Menopause; D051379:Mice; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects*; D010052:Ovariectomy; D010758:Phosphorus / Q000097:blood
chemical_list: D010758:Phosphorus; D008274:Magnesium; D005944:Glucosamine; D002118:Calcium