title: Detection and Quantification of Chikungunya Virus by Real-Time RT-PCR Assay.
abstract: Real-time PCR assay has many advantages over conventional PCR methods, including rapidity, quantitative measurement, low risk of contamination, high sensitivity, high specificity, and ease of standardization (Mackay et al., Nucleic Acids Res 30:1292-1305, 2002). The real-time PCR system relies upon the measurement of a fluorescent reporter during PCR, in which the amount of emitted fluorescence is directly proportional to the amount of the PCR product in a reaction (Gibsons et al., Genome Res 6:995-1001, 1996). Here, we describe the use of SYBR Green I-based and TaqMan(®) real-time reverse transcription polymerase chain reaction (RT-PCR) for the detection and quantification of Chikungunya virus (CHIKV).
authors.affiliation: Faculty of Medicine, Jalan Hospital, Universiti Teknologi MARA (UiTM), Sungai Buloh Campus, 47000 Sungai Buloh, Selangor Darul Ehsan, Malaysia. wangsm@salam.uitm.edu.my.
authors.forename: Seok Mui
authors.initials: SM
authors.lastname: Wang
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, University of Malaysia, Jalan Universiti, Kuala Lumpur, 50603, Malaysia.|Virology Unit, Infectious Diseases Research Centre, Institute for Medical Research, Jalan Pahang, Kuala Lumpur, 50588, Malaysia.
authors.forename: Ummul Haninah
authors.initials: UH
authors.lastname: Ali
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, University of Malaysia, Jalan Universiti, Kuala Lumpur, 50603, Malaysia.
authors.forename: Shamala Devi
authors.initials: SD
authors.lastname: Sekaran
authors.affiliation: Virology Unit, Infectious Diseases Research Centre, Institute for Medical Research, Jalan Pahang, Kuala Lumpur, 50588, Malaysia.
authors.forename: Ravindran
authors.initials: R
authors.lastname: Thayan
mesh_terms: D000818:Animals; D052160:Benzothiazoles; D002646:Chikungunya virus / Q000235:genetics* / Q000302:isolation & purification*; D009033:Culicidae / Q000821:virology; D003959:Diamines; D005456:Fluorescent Dyes; D018628:Gene Dosage; D000882:Haplorhini / Q000821:virology; D006801:Humans; D009930:Organic Chemicals; D011804:Quinolines; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
chemical_list: D052160:Benzothiazoles; D003959:Diamines; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D011804:Quinolines; D012367:RNA, Viral; C098022:SYBR Green I
keywords: Chikungunya virus; Detection; Quantification; Real-time RT-PCR; SYBR Green I; TaqMan®