title: Molecular Genetic Characterization of an Anthrabenzoxocinones Gene Cluster in Streptomyces Sp. FJS31-2 for the Biosynthesis of BE-24566B and Zunyimycin Ale.
abstract: Genome mining is an effective tool used to discover novel natural products from actinomycetes. Genome sequence analysis of Streptomyces sp. FJS31-2 revealed the presence of one putative type II polyketide gene cluster (ABX), which may correspond to type II polyketide products including BE-24566B and its chloro-derivatives. The addition of natural humus acid successfully activated the biosynthsis of the abx gene cluster. BE-24566B and its chloro-derivatives, named zunyimycin A, were also detected. The targeted deletion of the polyketide skeleton synthesis genes such as abxp, abxk, and abxs was performed in the wild strain to identify the gene cluster for BE-24566B biosynthesis.
authors.affiliation: Guizhou Key Laboratory of Microbial Resources &amp; Drug Development, Zunyi Medical University, Zunyi, Guizhou 563003, China. 18585737637@163.com.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Lü
authors.affiliation: Guizhou Key Laboratory of Microbial Resources &amp; Drug Development, Zunyi Medical University, Zunyi, Guizhou 563003, China. changwuyue@hotmail.com.
authors.forename: Changwu
authors.initials: C
authors.lastname: Yue
authors.affiliation: Guizhou Key Laboratory of Microbial Resources &amp; Drug Development, Zunyi Medical University, Zunyi, Guizhou 563003, China. meiyun216@126.com.
authors.forename: Meiyun
authors.initials: M
authors.lastname: Shao
authors.affiliation: Guizhou Key Laboratory of Microbial Resources &amp; Drug Development, Zunyi Medical University, Zunyi, Guizhou 563003, China. qianshengyan@126.com.
authors.forename: Shengyan
authors.initials: S
authors.lastname: Qian
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. fussliu@126.com.
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
authors.affiliation: Guizhou Key Laboratory of Microbial Resources &amp; Drug Development, Zunyi Medical University, Zunyi, Guizhou 563003, China. baoyuxin8680915@126.com.
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Bao
authors.affiliation: Guizhou Key Laboratory of Microbial Resources &amp; Drug Development, Zunyi Medical University, Zunyi, Guizhou 563003, China. wangmiao_good@163.com.
authors.forename: Miao
authors.initials: M
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. lysf1987313@163.com.
authors.forename: Minghao
authors.initials: M
authors.lastname: Liu
authors.affiliation: Guizhou Key Laboratory of Microbial Resources &amp; Drug Development, Zunyi Medical University, Zunyi, Guizhou 563003, China. xiaoqianli0908@163.com.
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Li
authors.affiliation: Guizhou Key Laboratory of Microbial Resources &amp; Drug Development, Zunyi Medical University, Zunyi, Guizhou 563003, China. yinyinwang0530@126.com.
authors.forename: Yinyin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. huangy@im.ac.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000880:Anthraquinones / Q000378:metabolism; D023061:Gene Order; D055442:Metabolome; D055432:Metabolomics / Q000379:methods; D015394:Molecular Structure; D005810:Multigene Family; D061065:Polyketides / Q000737:chemistry / Q000378:metabolism*; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism*
chemical_list: D000880:Anthraquinones; C097807:BE 24566B; D061065:Polyketides
keywords: anthrabenzoxocinones; gene cluster; genetic engineering; genome mining; polyketide synthases