title: Protective Effects of Green Tea Polyphenol Against Renal Injury Through ROS-Mediated JNK-MAPK Pathway in Lead Exposed Rats.
abstract: To investigate the potential therapeutic effects of polyphenols in treating Pb induced renal dysfunction and intoxication and to explore the detailed underlying mechanisms. Wistar rats were divided into four groups: control groups (CT), Pb exposure groups (Pb), Pb plus Polyphenols groups (Pb+PP) and Polyphenols groups (PP). Animals were kept for 60 days and sacrificed for tests of urea, serum blood urea nitrogen (BUN) and creatinine. Histological evaluations were then performed. In vitro studies were performed using primary kidney mesangial cells to reveal detailed mechanisms. Cell counting kit-8 (CCK-8) was used to evaluate cell viability. Pb induced cell apoptosis was measured by flow cytometry. Reactive oxygen species (ROS) generation and scavenging were tested by DCFH-DA. Expression level of tumor necrosis factor-α (TNF-α), interleukin-1-β (IL-1-β) and IL-6 were assayed by ELISA. Western blot and qPCR were used to measure the expression of ERK1/2, JNK1/2 and p38. Polyphenols have obvious protective effects on Pb induced renal dysfunction and intoxication both in vivo and in vitro. Polyphenols reduced Pb concentration and accumulation in kidney. Polyphenols also protected kidney mesangial cells from Pb induced apoptosis. Polyphenols scavenged Pb induced ROS generation and suppressed ROS-mediated ERK/JNK/p38 pathway. Downstream pro-inflammatory cytokines were inhibited in consistency. Polyphenol is protective in Pb induced renal intoxication and inflammatory responses. The underlying mechanisms lie on the antioxidant activity and ROS scavenging activity of polyphenols.
authors.affiliation: College of Food and Biological Engineering, Hubei University of Technology, Huazhong Agricultural University, Wuhan, PR China.
authors.forename: Haidong
authors.initials: H
authors.lastname: Wang
authors.affiliation: Nutrition and Food Research Institute, Wuhan Economic College, Huazhong Agricultural University, Wuhan, PR China.
authors.forename: Deyuan
authors.initials: D
authors.lastname: Li
authors.affiliation: Nutrition and Food Research Institute, Wuhan Economic College, Huazhong Agricultural University, Wuhan, PR China.
authors.forename: Zhongze
authors.initials: Z
authors.lastname: Hu
authors.affiliation: College of Food Science and Technology, Huazhong Agricultural University, Wuhan, PR China.
authors.forename: Siming
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Nutrition and Food Research Institute, Wuhan Economic College, Huazhong Agricultural University, Wuhan, PR China.
authors.forename: Zhejun
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: College of Food and Biological Engineering, Hubei University of Technology, Huazhong Agricultural University, Wuhan, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
mesh_terms: D000818:Animals; D001806:Blood Urea Nitrogen; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003404:Creatinine / Q000032:analysis; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000187:drug effects; D007674:Kidney Diseases / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D007854:Lead / Q000633:toxicity*; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D008297:Male; D050527:Mesangial Cells / Q000166:cytology / Q000187:drug effects; D059808:Polyphenols / Q000008:administration & dosage* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D013662:Tea / Q000737:chemistry*
chemical_list: D059808:Polyphenols; D017382:Reactive Oxygen Species; D013662:Tea; D007854:Lead; D003404:Creatinine
keywords: ROS; lead; oxidative stress; polyphenols; renal inflammation