title: Light-Triggered Release of Biomolecules from Diamond Nanowire Electrodes.
abstract: The controlled release of biomolecules from a substrate surface is a challenging task. Photocleavable linkers appear as attractive candidates for light-triggered delivery. We show here the possibility of creating photoactivable diamond nanowire interfaces, from which molecules can be photochemically released upon irradiation at 365 nm for several minutes. The approach is based on the covalent modification of boron-doped diamond nanowires (BDD NWs) with o-nitrobenzyl containing ligands, to which different biomolecules can be attached via amide bond formation. The photodecomposition reaction and the subsequent release of small proteins such as lysozyme or enzymes such as horseradish peroxidase (HRP) are investigated using electrochemical impedance spectroscopy. Using a colorimetric assay, we demonstrate that, while complete cleavage of HRP was achieved upon irradiation for 10 min at 1 W cm(-2), this exposure time resulted in a partial loss of enzymatic activity.
authors.affiliation: Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR CNRS 8520, Lille 1 University , Avenue Poincaré-BP60069, 59652 Villeneuve d'Ascq, France.|Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials (Ministry of Education), Shandong University , Jinan 250061, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR CNRS 8520, Lille 1 University , Avenue Poincaré-BP60069, 59652 Villeneuve d'Ascq, France.
authors.forename: Yannick
authors.initials: Y
authors.lastname: Coffinier
authors.affiliation: Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials (Ministry of Education), Shandong University , Jinan 250061, China.
authors.forename: Musen
authors.initials: M
authors.lastname: Li
authors.affiliation: Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR CNRS 8520, Lille 1 University , Avenue Poincaré-BP60069, 59652 Villeneuve d'Ascq, France.
authors.forename: Rabah
authors.initials: R
authors.lastname: Boukherroub
authors.affiliation: Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR CNRS 8520, Lille 1 University , Avenue Poincaré-BP60069, 59652 Villeneuve d'Ascq, France.
authors.forename: Sabine
authors.initials: S
authors.lastname: Szunerits