title: Plasmonic gratings with nano-protrusions made by glancing angle deposition for single-molecule super-resolution imaging.
abstract: Super-resolution imaging has been advantageous in studying biological and chemical systems, but the required equipment and platforms are expensive and unable to observe single-molecules at the high (μM) fluorophore concentrations required to study protein interaction and enzymatic activity. Here, a plasmonic platform was designed that utilized an inexpensively fabricated plasmonic grating in combination with a scalable glancing angle deposition (GLAD) technique using physical vapor deposition. The GLAD creates an abundance of plasmonic nano-protrusion probes that combine the surface plasmon resonance (SPR) from the periodic gratings with the localized SPR of these nano-protrusions. The resulting platform enables simultaneous imaging of a large area without point-by-point scanning or bulk averaging for the detection of single Cyanine-5 molecules in dye concentrations ranging from 50 pM to 10 μM using epifluorescence microscopy. Combining the near-field plasmonic nano-protrusion probes and super-resolution technique using localization microscopy, we demonstrate the ability to resolve grain sizes down to 65 nm. This plasmonic GLAD grating is a cost-effective super-resolution imaging substrate with potential applications in high-speed biomedical imaging over a wide range of fluorescent concentrations.
authors.affiliation: Department of Electrical and Computer Engineering, 139 and 141A Engineering Building West, University of Missouri, Columbia, MO 65211, USA. gangopadhyays@missouri.edu.
authors.forename: B
authors.initials: B
authors.lastname: Chen
authors.affiliation: Department of Bioengineering, 139 and 141A Engineering Building West, University of Missouri, Columbia, MO 65211, USA.
authors.forename: A
authors.initials: A
authors.lastname: Wood
authors.affiliation: Department of Electrical and Computer Engineering, 139 and 141A Engineering Building West, University of Missouri, Columbia, MO 65211, USA. gangopadhyays@missouri.edu.
authors.forename: A
authors.initials: A
authors.lastname: Pathak
authors.affiliation: Department of Electrical and Computer Engineering, 139 and 141A Engineering Building West, University of Missouri, Columbia, MO 65211, USA. gangopadhyays@missouri.edu.
authors.forename: J
authors.initials: J
authors.lastname: Mathai
authors.affiliation: Department of Electrical and Computer Engineering, 139 and 141A Engineering Building West, University of Missouri, Columbia, MO 65211, USA. gangopadhyays@missouri.edu.
authors.forename: S
authors.initials: S
authors.lastname: Bok
authors.affiliation: Department of Electrical and Computer Engineering, 139 and 141A Engineering Building West, University of Missouri, Columbia, MO 65211, USA. gangopadhyays@missouri.edu.
authors.forename: H
authors.initials: H
authors.lastname: Zheng
authors.affiliation: Department of Electrical and Computer Engineering, 139 and 141A Engineering Building West, University of Missouri, Columbia, MO 65211, USA. gangopadhyays@missouri.edu.
authors.forename: S
authors.initials: S
authors.lastname: Hamm
authors.affiliation: Department of Electrical and Computer Engineering, 139 and 141A Engineering Building West, University of Missouri, Columbia, MO 65211, USA. gangopadhyays@missouri.edu.
authors.forename: S
authors.initials: S
authors.lastname: Basuray
authors.affiliation: Department of Bioengineering, 139 and 141A Engineering Building West, University of Missouri, Columbia, MO 65211, USA.
authors.forename: S
authors.initials: S
authors.lastname: Grant
authors.affiliation: Department of Electrical and Computer Engineering, 139 and 141A Engineering Building West, University of Missouri, Columbia, MO 65211, USA. gangopadhyays@missouri.edu.
authors.forename: K
authors.initials: K
authors.lastname: Gangopadhyay
authors.affiliation: Department of Biochemistry, 117 Schweitzer Hall, University of Missouri, Columbia, MO 65211, USA. cornishp@missouri.edu.
authors.forename: P V
authors.initials: PV
authors.lastname: Cornish
authors.affiliation: Department of Electrical and Computer Engineering, 139 and 141A Engineering Building West, University of Missouri, Columbia, MO 65211, USA. gangopadhyays@missouri.edu.
authors.forename: S
authors.initials: S
authors.lastname: Gangopadhyay