title: Pharmacokinetics and tissue distribution of five active ingredients of Eucommiae cortex in normal and ovariectomized mice by UHPLC-MS/MS.
abstract: 1. Pinoresinol di-O-β-d-glucopyranoside (PDG), geniposide (GE), geniposidic acid (GA), aucubin (AN) and chlorogenic acid (CA) are the representative active ingredients in Eucommiae cortex (EC), which may be estrogenic. 2. The ultra high-performance liquid chromatography/tandem mass spectrometry (UHPLC-MS/MS) method for simultaneous determination of the five ingredients showed good linearity, low limits of quantification and high extraction recoveries, as well as acceptable precision, accuracy and stability in mice plasma and tissue samples (liver, spleen, kidney and uterus). It was successfully applied to the comparative study on pharmacokinetics and tissue distribution of PDG, GE, GA, AN and CA between normal and ovariectomized (OVX) mice. 3. The results indicated that except CA, the plasma and tissue concentrations of PDG, GE, GA in OVX mice were all greater than those in normal mice. AN could only be detected in the plasma and liver homogenate of normal mice, which was poorly absorbed in OVX mice and low in other measured tissues. PDG, GE and GA seem to be better absorbed in OVX mice than in normal mice proved by the remarkable increased value of AUC0-∞ and Cmax. It is beneficial that PDG, GE, GA have better plasma absorption and tissue distribution in pathological state.
authors.affiliation: a School of Pharmacy, Lanzhou University , Lanzhou , PR China .|b The MOE Key Laboratory for Standardization of Chinese Medicines and the Key Laboratory for Pharmacology of Compound Chinese Medicine of Shanghai, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine , Shanghai , PR China .|c Hong-Hui Hospital, Xi'an Jiaotong University College of Medicine , Xi'an , PR China , and.
authors.forename: Jing
authors.initials: J
authors.lastname: An
authors.affiliation: a School of Pharmacy, Lanzhou University , Lanzhou , PR China .|b The MOE Key Laboratory for Standardization of Chinese Medicines and the Key Laboratory for Pharmacology of Compound Chinese Medicine of Shanghai, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine , Shanghai , PR China .
authors.forename: Fangdi
authors.initials: F
authors.lastname: Hu
authors.affiliation: b The MOE Key Laboratory for Standardization of Chinese Medicines and the Key Laboratory for Pharmacology of Compound Chinese Medicine of Shanghai, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine , Shanghai , PR China .|d Research Center for Traditional Chinese Medicine of Complexity System, Shanghai University of Traditional Chinese Medicine , Shanghai , PR China.
authors.forename: Changhong
authors.initials: C
authors.lastname: Wang
authors.affiliation: b The MOE Key Laboratory for Standardization of Chinese Medicines and the Key Laboratory for Pharmacology of Compound Chinese Medicine of Shanghai, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine , Shanghai , PR China .|d Research Center for Traditional Chinese Medicine of Complexity System, Shanghai University of Traditional Chinese Medicine , Shanghai , PR China.
authors.forename: Zijia
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: b The MOE Key Laboratory for Standardization of Chinese Medicines and the Key Laboratory for Pharmacology of Compound Chinese Medicine of Shanghai, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine , Shanghai , PR China .|d Research Center for Traditional Chinese Medicine of Complexity System, Shanghai University of Traditional Chinese Medicine , Shanghai , PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.affiliation: b The MOE Key Laboratory for Standardization of Chinese Medicines and the Key Laboratory for Pharmacology of Compound Chinese Medicine of Shanghai, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine , Shanghai , PR China .|d Research Center for Traditional Chinese Medicine of Complexity System, Shanghai University of Traditional Chinese Medicine , Shanghai , PR China.
authors.forename: Zhengtao
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D002726:Chlorogenic Acid / Q000493:pharmacokinetics; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics*; D004967:Estrogens / Q000493:pharmacokinetics; D005960:Glucosides / Q000493:pharmacokinetics; D057888:Iridoid Glucosides / Q000493:pharmacokinetics; D039823:Iridoids / Q000493:pharmacokinetics; D017705:Lignans / Q000493:pharmacokinetics; D051379:Mice; D010052:Ovariectomy; D014018:Tissue Distribution
chemical_list: D004365:Drugs, Chinese Herbal; D004967:Estrogens; D005960:Glucosides; D057888:Iridoid Glucosides; D039823:Iridoids; D017705:Lignans; C000591465:pinoresinol-4,4'-di-O-beta-D-glucopyranoside; C007835:geniposide; C058966:geniposidic acid; C006650:aucubin; D002726:Chlorogenic Acid
keywords: Bioanalysis; Eucommiae cortex; UHPLC-MS/MS; ovariectomized mice; pharmacokinetics; tissue distribution