title: Reconstruction of a chromatic response system in Escherichia coli.
abstract: Two-component signal transduction systems (TCS) are involved in widespread cellular responses to diverse signals from bacteria to plants. Cyanobacteria have evolved photoperception systems for efficient photosynthesis, and some histidine kinases are known to function as photosensors. In this study, we attempt to reconstruct the photoperception system in Escherichia coli to make an easily controllable ON/OFF switch for gene expressions. For this purpose, a CcaS-CcaR two-component system from Nostoc punctiforme was expressed with phycocyanobilin (PCB) producing enzymes in E. coli which carries a G-box-controlled reporter gene. We succeeded to endow E. coli with a gene activation switch that is regulated in a light-color dependent manner. The possibility of such a switch for the development of synthetic biology is pointed out.
authors.affiliation: Laboratory of Molecular Microbiology, Graduate School of Bioagricultural Sciences, Nagoya University.
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Sugie
authors.forename: Mayuko
authors.initials: M
authors.lastname: Hori
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Oka
authors.forename: Hokuto
authors.initials: H
authors.lastname: Ohtsuka
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Aiba
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D005818:Genetic Engineering / Q000379:methods*; D008027:Light; D055537:Light Signal Transduction; D046937:Nostoc / Q000235:genetics; D020283:Photoreceptors, Microbial / Q000235:genetics / Q000378:metabolism*; D010788:Photosynthesis / Q000235:genetics; D052980:Phycobilins / Q000378:metabolism; D010798:Phycocyanin / Q000378:metabolism; D058615:Synthetic Biology / Q000379:methods
chemical_list: D001426:Bacterial Proteins; D020283:Photoreceptors, Microbial; D052980:Phycobilins; D010798:Phycocyanin; C011069:phycocyanobilin