title: Expression and Function of Group IIE Phospholipase A2 in Mouse Skin.
abstract: Recent studies using knock-out mice for various secreted phospholipase A2 (sPLA2) isoforms have revealed their non-redundant roles in diverse biological events. In the skin, group IIF sPLA2 (sPLA2-IIF), an "epidermal sPLA2" expressed in the suprabasal keratinocytes, plays a fundamental role in epidermal-hyperplasic diseases such as psoriasis and skin cancer. In this study, we found that group IIE sPLA2 (sPLA2-IIE) was expressed abundantly in hair follicles and to a lesser extent in basal epidermal keratinocytes in mouse skin. Mice lacking sPLA2-IIE exhibited skin abnormalities distinct from those in mice lacking sPLA2-IIF, with perturbation of hair follicle ultrastructure, modest changes in the steady-state expression of a subset of skin genes, and no changes in the features of psoriasis or contact dermatitis. Lipidomics analysis revealed that sPLA2-IIE and -IIF were coupled with distinct lipid pathways in the skin. Overall, two skin sPLA2s, hair follicular sPLA2-IIE and epidermal sPLA2-IIF, play non-redundant roles in distinct compartments of mouse skin, underscoring the functional diversity of multiple sPLA2s in the coordinated regulation of skin homeostasis and diseases.
authors.affiliation: From the Lipid Metabolism Project and the Faculty of Bioscience and Bioindustry, Tokushima University, Tokushima 770-8513, Japan, PRIME and.
authors.forename: Kei
authors.initials: K
authors.lastname: Yamamoto
authors.affiliation: From the Lipid Metabolism Project and.
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Miki
authors.affiliation: From the Lipid Metabolism Project and.
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Sato
authors.affiliation: Core Technology and Research Center, Tokyo Metropolitan Institute of Medical Science, Tokyo 156-8506, Japan.
authors.forename: Yasumasa
authors.initials: Y
authors.lastname: Nishito
authors.affiliation: the Departments of Chemistry and Biochemistry, University of Washington, Seattle, Washington 98195, and.
authors.forename: Michael H
authors.initials: MH
authors.lastname: Gelb
authors.affiliation: From the Lipid Metabolism Project and.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Taketomi
authors.affiliation: From the Lipid Metabolism Project and AMED-CREST, Japan Agency for Medical Research and Development, Tokyo 100-0004, Japan murakami-mk@igakuken.or.jp.
authors.forename: Makoto
authors.initials: M
authors.lastname: Murakami
mesh_terms: D000818:Animals; D003877:Dermatitis, Contact / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D004817:Epidermis / Q000201:enzymology / Q000473:pathology; D015971:Gene Expression Regulation, Enzymologic; D054501:Group II Phospholipases A2 / Q000096:biosynthesis* / Q000235:genetics; D018859:Hair Follicle / Q000201:enzymology* / Q000473:pathology; D051379:Mice; D018345:Mice, Knockout; D011565:Psoriasis / Q000201:enzymology* / Q000235:genetics / Q000473:pathology
chemical_list: D054501:Group II Phospholipases A2; C516295:Pla2g2e protein, mouse; C516297:Pla2g2f protein, mouse
keywords: Phospholipase A; fatty acid; lipid metabolism; lysophospholipid; skin