title: [Peculiarities of detection of 4-nitro-3-(trifluoromethyl)-aniline in the biological material].
abstract: The objective of the present work was to study peculiarities of detection of 4-nitro-3-(trifluoromethyl)-aniline in the biological material with the use of TLC, GC-MS, and electron spectrophotometry. We have proposed the rationale for the application of acetone as an insulating agent for the extraction of 4-nitro-3-(trifluoromethyl)-aniline from the cadaveric hepatic tissue and biological fluids. It was shown that this compound is possible to separate from endogenous biomaterials on the silicagel L column (40/100 mcm). The results of the quantitative evaluation of different amounts of 4-nitro-3-(trifluoromethyl)-aniline in the cadaveric hepatic tissue, blood, plasma, and urine are presented. The proposed method makes it possible to determine a minimum of 0.12 mg of 4-nitro-3-(trifluoromethyl)-aniline in 100 g of the biological material (cadaveric hepatic tissue), 0.09 mg in 100 g of blood, 0.06 mg and 0.05 mg in 100 u of plasma and urine respectively.
authors.affiliation: Kursk State Medical University, Kursk, Russia, 305041.
authors.forename: V K
authors.initials: VK
authors.lastname: Shormanov
authors.affiliation: Kursk State Medical University, Kursk, Russia, 305041.
authors.forename: Yu V
authors.initials: YV
authors.lastname: Andreeva
authors.affiliation: Experimental Criminalistic Centre, Orel Regional Department of Internal Affairs, Orel, Russia, 302028.
authors.forename: V A
authors.initials: VA
authors.lastname: Omel'chenko
mesh_terms: D000096:Acetone / Q000494:pharmacology*; D000814:Aniline Compounds / Q000032:analysis / Q000737:chemistry / Q000506:poisoning; D002855:Chromatography, Thin Layer / Q000379:methods; D053593:Forensic Toxicology / Q000379:methods; D006801:Humans; D008099:Liver / Q000473:pathology*; D011041:Poisoning / Q000097:blood / Q000209:etiology / Q000473:pathology / Q000652:urine; D012997:Solvents / Q000494:pharmacology; D013055:Spectrophotometry, Infrared / Q000379:methods
chemical_list: D000814:Aniline Compounds; D012997:Solvents; D000096:Acetone