title: Platelet lysate and chondroitin sulfate loaded contact lenses to heal corneal lesions.
abstract: Hemoderivative tear substitutes contain various ephiteliotrophic factors, such as growth factors (GF), involved in ocular surface homeostasis without immunogenic properties. The aim of the present work was the loading of platelet lysate into contact lenses to improve the precorneal permanence of platelet lysate growth factors on the ocular surface to enhance the treatment of corneal lesions. To this purpose, chondroitin sulfate, a sulfated glycosaminoglycan, which is normally present in the extracellular matrix, was associated with platelet lysate. In fact, chondroitin sulfate is capable of electrostatic interaction with positively charged growth factors, in particular, with bFGF, IGF, VEGF, PDGF and TGF-β, resulting in their stabilization and reduced degradation in solution. In the present work, various types of commercially available contact lenses have been loaded with chondroitin sulfate or chondroitin sulfate in association with platelet lysate to achieve a release of growth factors directly onto the corneal surface lesions. One type of contact lenses (PureVision(®)) showed in vitro good proliferation properties towards corneal cells and were able to enhance cut closure in cornea constructs.
authors.affiliation: Department of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, Italy. Electronic address: giuseppina.sandri@unipv.it.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Sandri
authors.affiliation: Department of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, Italy.
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Bonferoni
authors.affiliation: Department of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Rossi
authors.affiliation: Azienda Ospedaliera della Provincia di Lodi, Largo Donatori del Sangue 1, 26900 Lodi, Italy.
authors.forename: Alessio
authors.initials: A
authors.lastname: Delfino
authors.affiliation: Department of Public Health, Experimental and Forensic Medicine, University of Pavia, via Forlanini 2, 27100 Pavia, Italy.
authors.forename: Federica
authors.initials: F
authors.lastname: Riva
authors.affiliation: Department of Public Health, Experimental and Forensic Medicine, University of Pavia, via Forlanini 2, 27100 Pavia, Italy.
authors.forename: Antonia
authors.initials: A
authors.lastname: Icaro Cornaglia
authors.affiliation: Department of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, Italy.
authors.forename: Giorgio
authors.initials: G
authors.lastname: Marrubini
authors.affiliation: Department of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, Italy.
authors.forename: Giorgio
authors.initials: G
authors.lastname: Musitelli
authors.affiliation: Immunohaematology and Transfusion Service, Apheresis and Cell Therapy Unit, IRCCS Policlinico San Matteo, 27100 Pavia, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Del Fante
authors.affiliation: Immunohaematology and Transfusion Service, Apheresis and Cell Therapy Unit, IRCCS Policlinico San Matteo, 27100 Pavia, Italy.
authors.forename: Cesare
authors.initials: C
authors.lastname: Perotti
authors.affiliation: Department of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, Italy.
authors.forename: Carla
authors.initials: C
authors.lastname: Caramella
authors.affiliation: Department of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, Italy.
authors.forename: Franca
authors.initials: F
authors.lastname: Ferrari
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000008:administration & dosage*; D003261:Contact Lenses; D003315:Cornea / Q000187:drug effects*; D003316:Corneal Diseases / Q000188:drug therapy*; D005109:Extracellular Matrix / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000008:administration & dosage*; D011817:Rabbits; D014945:Wound Healing / Q000187:drug effects
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D002809:Chondroitin Sulfates
keywords: Chondroitin sulfate; Contact lenses; Platelet lysate; in vitro wound healing