title: IgE Sensitization Profiles Differ between Adult Patients with Severe and Moderate Atopic Dermatitis.
abstract: Atopic dermatitis (AD) is a complex chronic inflammatory disease where allergens can act as specific triggering factors.
  
  
  
  To characterize the specificities of IgE-reactivity in patients with AD to a broad panel of exogenous allergens including microbial and human antigens.
  
  
  
  Adult patients with AD were grouped according to the SCORAD index, into severe (n = 53) and moderate AD (n = 126). As controls 43 patients were included with seborrhoeic eczema and 97 individuals without history of allergy or skin diseases. Specific IgE reactivity was assessed in plasma using Phadiatop®, ImmunoCap™, micro-arrayed allergens, dot-blotted recombinant Malassezia sympodialis allergens, and immune-blotted microbial and human proteins.
  
  
  
  IgE reactivity was detected in 92% of patients with severe and 83% of patients with moderate AD. Sensitization to cat allergens occurred most frequently, followed by sensitization to birch pollen, grass pollen, and to the skin commensal yeast M. sympodialis. Patients with severe AD showed a significantly higher frequency of IgE reactivity to allergens like cat (rFel d 1) and house dust mite (rDer p 4 and 10), to Staphylococcus aureus, M. sympodialis, and to human antigens. In contrast, there were no significant differences in the frequencies of IgE reactivity to the grass pollen allergens rPhl p 1, 2, 5b, and 6 between the two AD groups. Furthermore the IgE reactivity profile of patients with severe AD was more spread towards several different allergen molecules as compared to patients with moderate AD.
  
  
  
  We have revealed a hitherto unknown difference regarding the molecular sensitization profile in patients with severe and moderate AD. Molecular profiling towards allergen components may provide a basis for future investigations aiming to explore the environmental, genetic and epigenetic factors which could be responsible for the different appearance and severity of disease phenotypes in AD.
authors.affiliation: Christian Doppler Laboratory for the Development of Allergen Chips, Medical University of Vienna, Vienna, Austria.|Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
authors.forename: Irene
authors.initials: I
authors.lastname: Mittermann
authors.affiliation: Department of Medicine Solna, Karolinska Institutet, and Dermatology and Venereology Unit, Karolinska University Hospital, Stockholm, Sweden.
authors.forename: Gustav
authors.initials: G
authors.lastname: Wikberg
authors.affiliation: Department of Clinical Science and Education, Karolinska Institutet, and Sachs' Children and Youth Hospital, SE-118 83 Södersjukhuset, Stockholm, Sweden.
authors.forename: Catharina
authors.initials: C
authors.lastname: Johansson
authors.affiliation: Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
authors.forename: Christian
authors.initials: C
authors.lastname: Lupinek
authors.affiliation: Department of Medicine Solna, Karolinska Institutet, and Dermatology and Venereology Unit, Karolinska University Hospital, Stockholm, Sweden.
authors.forename: Lena
authors.initials: L
authors.lastname: Lundeberg
authors.affiliation: Swiss Institute of Allergy and Asthma Research (SIAF), University of Zurich, Davos, Switzerland.
authors.forename: Reto
authors.initials: R
authors.lastname: Crameri
authors.affiliation: Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
authors.forename: Rudolf
authors.initials: R
authors.lastname: Valenta
authors.affiliation: Department of Clinical Science and Education, Karolinska Institutet, and Sachs' Children and Youth Hospital, SE-118 83 Södersjukhuset, Stockholm, Sweden.
authors.forename: Annika
authors.initials: A
authors.lastname: Scheynius
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000485:Allergens / Q000276:immunology*; D000818:Animals; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D002415:Cats; D003876:Dermatitis, Atopic / Q000097:blood / Q000175:diagnosis / Q000276:immunology*; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology*; D008290:Malassezia / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011994:Recombinant Proteins / Q000276:immunology; D012882:Skin Tests; D013211:Staphylococcus aureus / Q000276:immunology*; D055815:Young Adult
chemical_list: D000485:Allergens; D015415:Biomarkers; D011994:Recombinant Proteins; D007073:Immunoglobulin E