title: Curcumin Attenuated Bupivacaine-Induced Neurotoxicity in SH-SY5Y Cells Via Activation of the Akt Signaling Pathway.
abstract: Bupivacaine is widely used for regional anesthesia, spinal anesthesia, and pain management. However, bupivacaine could cause neuronal injury. Curcumin, a low molecular weight polyphenol, has a variety of bioactivities and may exert neuroprotective effects against damage induced by some stimuli. In the present study, we tested whether curcumin could attenuate bupivacaine-induced neurotoxicity in SH-SY5Y cells. Cell injury was evaluated by examining cell viability, mitochondrial damage and apoptosis. We also investigated the levels of activation of the Akt signaling pathway and the effect of Akt inhibition by triciribine on cell injury following bupivacaine and curcumin treatment. Our findings showed that the bupivacaine treatment could induce neurotoxicity. Pretreatment of the SH-SY5Y cells with curcumin significantly attenuated bupivacaine-induced neurotoxicity. Interestingly, the curcumin treatment increased the levels of Akt phosphorylation. More significantly, the pharmacological inhibition of Akt abolished the cytoprotective effect of curcumin against bupivacaine-induced cell injury. Our data suggest that pretreating SH-SY5Y cells with curcumin provides a protective effect on bupivacaine-induced neuronal injury via activation of the Akt signaling pathway.
authors.affiliation: Department of Anesthesiology, Zhujiang Hospital, Southern Medical University, 253 Industrial Road, Guangzhou, 510282, Guangdong Province, China.|Department of Anesthesiology, Central Hospital of Panyu District, Guangzhou, 511400, Guangdong Province, China.
authors.forename: You-Ling
authors.initials: YL
authors.lastname: Fan
authors.affiliation: Department of Anesthesiology, Zhujiang Hospital, Southern Medical University, 253 Industrial Road, Guangzhou, 510282, Guangdong Province, China.|Department of Anesthesiology, Guangzhou First People's Hospital, Guangzhou, 510180, Guangdong Province, China.
authors.forename: Heng-Chang
authors.initials: HC
authors.lastname: Li
authors.affiliation: Department of Anesthesiology, Zhujiang Hospital, Southern Medical University, 253 Industrial Road, Guangzhou, 510282, Guangdong Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Anesthesiology, Central Hospital of Panyu District, Guangzhou, 511400, Guangdong Province, China.
authors.forename: Hui-Hua
authors.initials: HH
authors.lastname: Peng
authors.affiliation: Department of Anesthesiology, Central Hospital of Panyu District, Guangzhou, 511400, Guangdong Province, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Huang
authors.affiliation: Department of Anesthesiology, Central Hospital of Panyu District, Guangzhou, 511400, Guangdong Province, China.
authors.forename: Wei-Hang
authors.initials: WH
authors.lastname: Jiang
authors.affiliation: Department of Anesthesiology, Zhujiang Hospital, Southern Medical University, 253 Industrial Road, Guangzhou, 510282, Guangdong Province, China. xushiyuan355@163.com.
authors.forename: Shi-Yuan
authors.initials: SY
authors.lastname: Xu
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002045:Bupivacaine / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects*; D003474:Curcumin / Q000494:pharmacology*; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology; D020258:Neurotoxicity Syndromes / Q000188:drug therapy* / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*
chemical_list: D018696:Neuroprotective Agents; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D003474:Curcumin; D002045:Bupivacaine
keywords: Akt; Curcumin; Local anesthetics; Neurotoxicity; SH-SY5Y cells