title: Linoelaidic acid enhances adipogenic differentiation in adipose tissue-derived stromal cells through suppression of Wnt/β-catenin signaling pathway in vitro.
abstract: Obesity has become a major health problem which is related with high-trans fatty acids diet. Adipogenic differentiation of adipose tissue-derived stromal cells (ADSCs) plays an important role in the development of adipose tissue. In order to determine the effect of trans fatty acids on adipogenic differentiation in ADSCs, cells were treated with linoelaidic acid, as well as linoleic acid and linolenic acid. We found that linoelaidic acid significantly increased the lipid droplet formation and triglyceride content compared with linoleic acid and linolenic acid. Linoelaidic acid also down-regulated the levels of β-catenin in cells and inhibited the accumulation of β-catenin in cell nuclei. Lithium chloride, an activator of Wnt/β-catenin pathway, antagonized the enhancement of linoelaidic acid on adipogenesis and up-regulated the levels of β-catenin in ADSCs. These results indicated that linoelaidic acid could enhance the adipogenic differentiation in ADSCs in vitro, which is partly due to the suppression of Wnt/β-catenin pathway.
authors.affiliation: School of Biological Engineering, Dalian Polytechnic University, No.1 Qinggong Road, Ganjingzi District, Dalian 116034, Liaoning, China.
authors.forename: Jihui
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, No.1 Qinggong Road, Ganjingzi District, Dalian 116034, Liaoning, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liang
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, No.1 Qinggong Road, Ganjingzi District, Dalian 116034, Liaoning, China.
authors.forename: Luyang
authors.initials: L
authors.lastname: Jian
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, No.1 Qinggong Road, Ganjingzi District, Dalian 116034, Liaoning, China.
authors.forename: Jingwei
authors.initials: J
authors.lastname: Zhang
authors.affiliation: School of Food Science and Technology, Dalian Polytechnic University, No.1 Qinggong Road, Ganjingzi District, Dalian 116034, Liaoning, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Liang
authors.affiliation: School of Biological Engineering, Dalian Polytechnic University, No.1 Qinggong Road, Ganjingzi District, Dalian 116034, Liaoning, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Xiao
authors.affiliation: School of Biological Engineering, Dalian Polytechnic University, No.1 Qinggong Road, Ganjingzi District, Dalian 116034, Liaoning, China.
authors.forename: Bingnan
authors.initials: B
authors.lastname: Liu
authors.affiliation: School of Biological Engineering, Dalian Polytechnic University, No.1 Qinggong Road, Ganjingzi District, Dalian 116034, Liaoning, China. Electronic address: hwang@dlpu.edu.cn.
authors.forename: Han
authors.initials: H
authors.lastname: Wang
mesh_terms: D041721:3T3-L1 Cells; D050156:Adipogenesis; D000273:Adipose Tissue / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D015536:Down-Regulation; D019787:Linoleic Acid / Q000494:pharmacology*; D051379:Mice; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism; D060449:Wnt Signaling Pathway / Q000187:drug effects*; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D014280:Triglycerides; D051176:beta Catenin; D019787:Linoleic Acid
keywords: Adipogenic differentiation; Adipose tissue-derived stromal cells; Linoelaidic acid; Trans fatty acids; Wnt/β-catenin pathway