title: Curcumin represses the activity of inhibitor-κB kinase in dextran sulfate sodium-induced colitis by S-nitrosylation.
abstract: In this study, we investigated the preventive effects of curcumin using dextran sulfate sodium (DSS)-induced colitis and the potential role of curcumin in regulation of anti-inflammation through S-nitrosylation. After curcumin treatment for 6days, the body weight and disease activity index of DSS-induced mice was alleviated and the colonic length was also rescued. Western blot presented that the protein expression of iNOS can be reduced by curcumin. Consistently, mRNA level of iNOS and pro-inflammatory cytokines, such as TNFα, IL-1β, and IL-6, was also repressed. Moreover, Curcumin reduced the amount of nitrite in DSS-induced colitis but not affected total S-nitrosylation level on proteins on day 6, indicating that curcumin inhibited NO oxidation. Furthermore, the protection of S-nitrosylation on IKKβ in DSS-induced colitis for 6days by curcumin caused the repression of IκB phosphorylation and NF-κB activation. In conclusion, this study verified that curcumin-mediated S-nitrosylation may be as an important regulator for anti-inflammation in DSS-induced colitis of mice.
authors.affiliation: Department of Food Science, University of National Taiwan Ocean University, Keelung, Taiwan.
authors.forename: Ning-Jo
authors.initials: NJ
authors.lastname: Kao
authors.affiliation: Department of Food Science, University of National Taiwan Ocean University, Keelung, Taiwan.
authors.forename: Jia-Yuan
authors.initials: JY
authors.lastname: Hu
authors.affiliation: Department of Food Science, University of National Taiwan Ocean University, Keelung, Taiwan.
authors.forename: Chien-Sheng
authors.initials: CS
authors.lastname: Wu
authors.affiliation: Department of Food Science, University of National Taiwan Ocean University, Keelung, Taiwan. Electronic address: kongzl@mail.ntou.edu.tw.
authors.forename: Zwe-Ling
authors.initials: ZL
authors.lastname: Kong
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy*; D003106:Colon / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D003474:Curcumin / Q000627:therapeutic use*; D016207:Cytokines / Q000378:metabolism; D016264:Dextran Sulfate; D051550:I-kappa B Kinase / Q000378:metabolism; D018836:Inflammation Mediators / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism*; D009589:Nitrogen Oxides / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D018836:Inflammation Mediators; D016328:NF-kappa B; D009589:Nitrogen Oxides; D016264:Dextran Sulfate; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; D051550:I-kappa B Kinase; D003474:Curcumin
keywords: Anti-inflammatory; Curcumin; Inducible nitric oxide synthase; Inhibitor-κB kinase; Phosphorylation; S-nitrosylation