title: Construction of Large-Volume Tissue Mimics with 3D Functional Vascular Networks.
abstract: We used indirect stereolithography (SL) to form inner-layered fluidic networks in a porous scaffold by introducing a hydrogel barrier on the luminal surface, then seeded the networks separately with human umbilical vein endothelial cells and human lung fibroblasts to form a tissue mimic containing vascular networks. The artificial vascular networks provided channels for oxygen transport, thus reducing the hypoxic volume and preventing cell death. The endothelium of the vascular networks significantly retarded the occlusion of channels during whole-blood circulation. The tissue mimics have the potential to be used as an in vitro platform to examine the physiologic and pathologic phenomena through vascular architecture.
authors.affiliation: Department of Biomedical Engineering, Yale University, New Haven, Connecticut, United States of America.
authors.forename: Tae-Yun
authors.initials: TY
authors.lastname: Kang
authors.affiliation: Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Gyungbuk, Korea.
authors.forename: Jung Min
authors.initials: JM
authors.lastname: Hong
authors.affiliation: Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Gyungbuk, Korea.
authors.forename: Jin Woo
authors.initials: JW
authors.lastname: Jung
authors.affiliation: Department of Biomedical Engineering, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), Ulsan, Korea.
authors.forename: Hyun-Wook
authors.initials: HW
authors.lastname: Kang
authors.affiliation: Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Gyungbuk, Korea.
authors.forename: Dong-Woo
authors.initials: DW
authors.lastname: Cho
mesh_terms: D000818:Animals; D040761:Biomimetic Materials; D001808:Blood Vessels; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D008955:Models, Cardiovascular; D023822:Tissue Engineering / Q000379:methods*