title: Transfection of Tumor-Infiltrating T Cells with mRNA Encoding CXCR2.
abstract: Adoptive T-cell therapy based on the infusion of patient's own immune cells after ex vivo culturing is among the most potent forms of personalized treatment among recent clinical developments for the treatment of cancer. However, despite high rates of successful initial clinical responses, only about 20 % of patients with metastatic melanoma treated with tumor-infiltrating lymphocytes (TILs) enter complete and long-term regression, with the majority either relapsing after initial partial regression or not benefiting at all. Previous studies have shown a positive correlation between the number infused T cells migrating to the tumor and the clinical response, but also that only a small fraction of adoptively transferred T cells reach the tumor site. In this chapter, we describe a protocol for transfection of TILs with mRNA encoding the chemokine receptor CXCR2 transiently redirecting and improving TILs migration toward tumor-secreted chemokines in vitro.
authors.affiliation: Center for Cancer Immune Therapy (CCIT), Department of Hematology, Copenhagen University Hospital Herlev, Herlev Ringvej 75, 2730, Herlev, Denmark. manja.idorn@regionh.dk.
authors.forename: Manja
authors.initials: M
authors.lastname: Idorn
authors.affiliation: Center for Cancer Immune Therapy (CCIT), Department of Hematology, Copenhagen University Hospital Herlev, Herlev Ringvej 75, 2730, Herlev, Denmark.
authors.forename: Per
authors.initials: P
authors.lastname: Thor Straten
authors.affiliation: Center for Cancer Immune Therapy (CCIT), Department of Hematology, Copenhagen University Hospital Herlev, Herlev Ringvej 75, 2730, Herlev, Denmark.|Department of Oncology, Copenhagen University Hospital Herlev, Herlev Ringvej 75, 2730, Herlev, Denmark.
authors.forename: Inge Marie
authors.initials: IM
authors.lastname: Svane
authors.affiliation: Center for Cancer Immune Therapy (CCIT), Department of Hematology, Copenhagen University Hospital Herlev, Herlev Ringvej 75, 2730, Herlev, Denmark.|Department of Oncology, Copenhagen University Hospital Herlev, Herlev Ringvej 75, 2730, Herlev, Denmark.
authors.forename: Özcan
authors.initials: Ö
authors.lastname: Met
mesh_terms: D019264:Adoptive Transfer; D018274:Electroporation; D006801:Humans; D007167:Immunotherapy; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology*; D008545:Melanoma / Q000276:immunology* / Q000628:therapy; D012333:RNA, Messenger / Q000235:genetics / Q000276:immunology*; D023063:Receptors, Interleukin-8B / Q000235:genetics / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D012333:RNA, Messenger; D023063:Receptors, Interleukin-8B
keywords: Cancer immunotherapy; Chemokine receptor; Tumor homing; Tumor-infiltrating lymphocytes (TILs); mRNA transfection