title: Preparation of positive blood cultures for direct MALDI-ToF MS identification.
abstract: MALDI-ToF MS can be used to identify microorganisms directly from blood cultures. This study compared two methods of sample preparation. Similar levels of genus- (91% vs 90%) and species-level identifications (79% vs 74%) were obtained with differential centrifugation and SDS methods. The SDS method is faster and requires minimal handling.
authors.affiliation: Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Robinson
authors.affiliation: Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand; Southern Community Laboratories, Dunedin, New Zealand. Electronic address: james.ussher@otago.ac.nz.
authors.forename: James E
authors.initials: JE
authors.lastname: Ussher
mesh_terms: D016470:Bacteremia / Q000175:diagnosis / Q000382:microbiology; D001419:Bacteria / Q000302:isolation & purification*; D000071997:Blood Culture / Q000191:economics / Q000379:methods*; D006801:Humans; D012967:Sodium Dodecyl Sulfate; D013048:Specimen Handling / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013997:Time Factors
chemical_list: D012967:Sodium Dodecyl Sulfate
keywords: Blood cultures; Bloodstream infections; MALDI-ToF MS; Rapid identification; Sepsis