title: Super elongation complex contains a TFIIF-related subcomplex.
abstract: Super elongation complex (SEC) belongs to a family of RNA polymerase II (Pol II) elongation factors that has similar properties as TFIIF, a general transcription factor that increases the transcription elongation rate by reducing pausing. Although SEC has TFIIF-like functional properties, it apparently lacks sequence and structural homology. Using HHpred, we find that SEC contains an evolutionarily related TFIIF-like subcomplex. We show that the SEC subunit ELL interacts with the Pol II Rbp2 subunit, as expected for a TFIIF-like factor. These findings suggest a new model for how SEC functions as a Pol II elongation factor and how it suppresses Pol II pausing.
authors.affiliation: a Department of Biochemistry and Molecular Biology , SUNY Upstate Medical University , Syracuse , NY , USA.
authors.forename: Bruce A
authors.initials: BA
authors.lastname: Knutson
authors.affiliation: a Department of Biochemistry and Molecular Biology , SUNY Upstate Medical University , Syracuse , NY , USA.
authors.forename: Marissa L
authors.initials: ML
authors.lastname: Smith
authors.affiliation: a Department of Biochemistry and Molecular Biology , SUNY Upstate Medical University , Syracuse , NY , USA.
authors.forename: Nancy
authors.initials: N
authors.lastname: Walker-Kopp
authors.affiliation: a Department of Biochemistry and Molecular Biology , SUNY Upstate Medical University , Syracuse , NY , USA.
authors.forename: Xia
authors.initials: X
authors.lastname: Xu
mesh_terms: D000595:Amino Acid Sequence; D006801:Humans; D008954:Models, Biological; D008958:Models, Molecular; D005810:Multigene Family; D046912:Multiprotein Complexes / Q000378:metabolism*; D010802:Phylogeny; D011485:Protein Binding; D011487:Protein Conformation; D054730:Protein Interaction Domains and Motifs; D012319:RNA Polymerase II / Q000378:metabolism; D061805:Transcription Elongation, Genetic; D019473:Transcription Factors, TFII / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D035602:Transcriptional Elongation Factors / Q000737:chemistry / Q000378:metabolism*
chemical_list: C090862:ELL protein, human; C447613:ELL2 protein, human; D046912:Multiprotein Complexes; D019473:Transcription Factors, TFII; D035602:Transcriptional Elongation Factors; D012319:RNA Polymerase II; C056604:transcription factor TFIIF
keywords: EAF; ELL; SEC; TFIIF; elongation; pausing