title: Hyaluronic acid enhances proliferation of human amniotic mesenchymal stem cells through activation of Wnt/β-catenin signaling pathway.
abstract: This study investigated the pro-proliferative effect of hyaluronic acid (HA) on human amniotic mesenchymal stem cells (hAMSCs) and the underlying mechanisms. Treatment with HA increased cell population growth in a dose- and time-dependent manner. Analyses by flow cytometry and immunocytochemistry revealed that HA did not change the cytophenotypes of hAMSCs. Additionally, the osteogenic, chondrogenic, and adipogenic differentiation capabilities of these hAMSCs were retained after HA treatment. Moreover, HA increased the mRNA expressions of wnt1, wnt3a, wnt8a, cyclin D1, Ki-67, and β-catenin as well as the protein level of β-catenin and cyclin D1 in hAMSCs; and the nuclear localization of β-catenin was also enhanced. Furthermore, the pro-proliferative effect of HA and up-regulated expression of Wnt/β-catenin pathway-associated proteins - wnt3a, β-catenin and cyclin D1 in hAMSCs were significantly inhibited upon pre-treatment with Wnt-C59, an inhibitor of the Wnt/β-catenin pathway. These results suggest that HA may positively regulate hAMSCs proliferation through regulation of the Wnt/β-catenin signaling pathway.
authors.affiliation: Guizhou Center for Translational Medicine, Affiliated Hospital of Zunyi Medical University, 149 Dalian Road, Zunyi 563000, PR China.
authors.forename: Ru-Ming
authors.initials: RM
authors.lastname: Liu
authors.affiliation: Guizhou Center for Translational Medicine, Affiliated Hospital of Zunyi Medical University, 149 Dalian Road, Zunyi 563000, PR China.
authors.forename: Ren-Gang
authors.initials: RG
authors.lastname: Sun
authors.affiliation: Guizhou Center for Translational Medicine, Affiliated Hospital of Zunyi Medical University, 149 Dalian Road, Zunyi 563000, PR China.
authors.forename: Ling-Tao
authors.initials: LT
authors.lastname: Zhang
authors.affiliation: Guizhou Center for Translational Medicine, Affiliated Hospital of Zunyi Medical University, 149 Dalian Road, Zunyi 563000, PR China.
authors.forename: Qing-Fang
authors.initials: QF
authors.lastname: Zhang
authors.affiliation: Guizhou Center for Translational Medicine, Affiliated Hospital of Zunyi Medical University, 149 Dalian Road, Zunyi 563000, PR China.
authors.forename: Dai-Xiong
authors.initials: DX
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Microbial Metabolism, and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, PR China. Electronic address: jjzhong@sjtu.edu.cn.
authors.forename: Jian-Jiang
authors.initials: JJ
authors.lastname: Zhong
authors.affiliation: Guizhou Center for Translational Medicine, Affiliated Hospital of Zunyi Medical University, 149 Dalian Road, Zunyi 563000, PR China. Electronic address: jhxiao@yahoo.com.
authors.forename: Jian-Hui
authors.initials: JH
authors.lastname: Xiao
mesh_terms: D000650:Amnion / Q000166:cytology*; D044482:Benzeneacetamides / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D019070:Cell Lineage / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D019938:Cyclin D1 / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D006820:Hyaluronic Acid / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D010012:Osteogenesis / Q000187:drug effects; D010641:Phenotype; D011247:Pregnancy; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D011725:Pyridines / Q000494:pharmacology; D060449:Wnt Signaling Pathway / Q000187:drug effects* / Q000235:genetics; D051176:beta Catenin / Q000378:metabolism
chemical_list: C579131:2-(4-(2-methylpyridin-4-yl)phenyl)-N-(4-(pyridin-3-yl)phenyl)acetamide; D044482:Benzeneacetamides; C530104:CCND1 protein, human; D016271:Proto-Oncogene Proteins c-myc; D011725:Pyridines; D051176:beta Catenin; D019938:Cyclin D1; D006820:Hyaluronic Acid
keywords: Human amniotic mesenchymal stem cell; Hyaluronic acid; Pro-proliferative effect; Proliferation; Wnt/β-catenin signaling pathway