title: [Changes of the mitochondrial DNA copy number and the antioxidant system in the PBMC of hepatocellular carcinoma].
abstract: OBJECTIVE
  To investigate the relationship between the changes of the copy numbers of mtDNA in peripheral blood mono-nucle- ar cell(PBMC) and the disordered of antioxidant capacity of hepatocellular carcinoma (HCC) patients.
  
  
  METHODS
  The Ficoll Hypaque method was used to isolate the PBMC from blood specimens. The ND1 gene of the mitochondrial was amplified by real-time PCR; meantime β-actin was served as a quantitative standard marker; the difference of mtDNA copy number in PBMC was compared between HCC and healthy control group. The level of reactive oxygen species (ROS) in PBMC was determined by flow cytometry. The change of total antioxidant capacity (T- AOC) of plasma was detected by the biochemistry examination.
  
  
  RESULTS
  The copy numbers of ND1 gene in PBMC of HCC was 73% that of the healthy control group,which suggested a decrease of the copy numbers of mtDNA in HCC. The levels of ROS of PBMC in HCC was (417. 82 ± 110.62) and (301.82 ± 75.54) in control group, which showed that the levels of ROS of PBMC in HCC were significant higher than that in control group (P < 0.01).Plasma T-AOC in HCC was (1.30 ± 0.85), and (3.20 ± 1.62) in control. The T-AOC of plasma of HCC was significantly lower than in control group (P < 0.01).
  
  
  CONCLUSIONS
  There was a certain relationship between the decrease of the copy numbers of mtDNA and the disordered antioxidant capacity in hepatocellular carcinoma, which may be associated with the development of hepatocellular carcinoma.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Gao
authors.forename: Hong-jing
authors.initials: HJ
authors.lastname: Nie
authors.forename: Dong
authors.initials: D
authors.lastname: Yang
authors.forename: Cheng-shi
authors.initials: CS
authors.lastname: Ding
authors.forename: Min
authors.initials: M
authors.lastname: Jin
authors.forename: Zhi-qiang
authors.initials: ZQ
authors.lastname: Chen
authors.forename: Zhi-gang
authors.initials: ZG
authors.lastname: Qiu
authors.forename: Xuan
authors.initials: X
authors.lastname: Guo
authors.forename: Zhao-lila
authors.initials: ZL
authors.lastname: Chen
authors.forename: Jun-wen
authors.initials: JW
authors.lastname: Li
mesh_terms: D000199:Actins; D000975:Antioxidants / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000097:blood / Q000235:genetics*; D016022:Case-Control Studies; D056915:DNA Copy Number Variations; D004272:DNA, Mitochondrial / Q000235:genetics*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008113:Liver Neoplasms / Q000097:blood / Q000235:genetics*; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D000199:Actins; D000975:Antioxidants; D004272:DNA, Mitochondrial; D017382:Reactive Oxygen Species