title: Association of IL-10 polymorphisms with hepatitis B virus infection and outcome in Han population.
abstract: BACKGROUND
  This study evaluated the correlation of single nucleotide polymorphisms interleukin (IL)-10-592 and -1082 with hepatitis B virus (HBV) susceptibility and recovery.
  
  
  METHODS
  Total 190 chronic hepatitis B (CHB) patients, 81 individuals with self-limited HBV infections and 81 normal controls from the first Hospital of Jilin University were recruited. The IL-10 polymorphisms were detected by polymerase chain reaction with restriction fragment length polymorphism (PCR-RFLP). The χ (2) test (p < 0.05) and Fisher's exact test were separately performed to analyze and compare the genotype frequencies of IL-10-592 and -1082 among different groups. Furthermore, logistic regression analysis (p < 0.05) was conducted to determine the correlation of genotypes with HBV infection and recovery. Genotype A/A, A/C and C/C of IL-10-592 had been detected in the three groups.
  
  
  RESULTS
  The frequencies of -592A separately were 55.56, 64.67 and 55.33 % in the three groups. Genotypes of IL-10-592 only had significant difference among the patients and normal controls (p = 0.021). Genotypes A/A, A/G and G/G of IL-10-1082 were detected in CHB patients and individuals with self-limited HBV infection; however, genotype G/G had not been detected in normal controls. The frequencies of -1082G separately were 3.68, 6.17 and 11.11 % in the three groups. Genotypes of IL-10-1082 only had no significant difference among the patients and individuals with self-limited HBV infections (p = 0.130). We found that their risks of HBV infection existed significant difference.
  
  
  CONCLUSIONS
  The IL-10-592 and -1082 polymorphisms might be associated with HBV infection, but not with the recovery after HBV infection.
authors.affiliation: Changchun University of Traditional Chinese Medicine, Changchun, 130117, Jilin, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Gao
authors.affiliation: Changchun University of Traditional Chinese Medicine, Changchun, 130117, Jilin, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: Changchun University of Traditional Chinese Medicine, Changchun, 130117, Jilin, China.
authors.forename: Lian
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Tumor Center, The 1st Hospital of Jilin University, Changchun, 130021, Jilin, China.
authors.forename: Di
authors.initials: D
authors.lastname: Wu
authors.affiliation: Changchun University of Traditional Chinese Medicine, Changchun, 130117, Jilin, China.
authors.forename: He
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Ministry of Education Key Laboratory of Zoonosis, The 1st Hospital of Jilin University, No. 71 Xinminda Street, Changchun, 130021, Jilin, China. junqiniu@aliyun.com.
authors.forename: Junqi
authors.initials: J
authors.lastname: Niu
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006515:Hepatitis B virus / Q000235:genetics*; D019694:Hepatitis B, Chronic / Q000453:epidemiology / Q000235:genetics* / Q000821:virology; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D008297:Male; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011379:Prognosis
chemical_list: C508609:IL10 protein, human; D016753:Interleukin-10
keywords: Hepatitis B virus; Interleukin-10; Single nucleotide polymorphism