title: Effects of glycerol, equilibration time and antioxidants on post-thaw functional integrity of bovine spermatozoa directly obtained from epididymis.
abstract: This work aimed to evaluate the effect of stabilisation times, glycerol concentration, and the catalase and superoxide dismutase supplementation of diluent on parameters of frozen-thawed spermatozoa from epididymis of Nelore bulls: Experiment 1: spermatozoa diluted in Tris-egg yolk with glycerol (3%, 5% or 7%) and stabilisation times (0, 2 or 4 hr at 5°C); Experiment 2: Tris-egg yolk only, Tris-egg yolk with catalase (CAT, 50 or 100 U ml-1) or superoxide dismutase (SOD, 50 or 100 U ml-1). Frozen-thawed spermatozoa were evaluated for kinetic parameters, plasma membrane and acrosome integrity, mitochondrial activity and IVF capacity. ALH and BCF were affected (p < .05) by glycerol at 3% after 4-hr equilibration time and 7% after 2-hr equilibration time. Glycerol 3% had lower (p < .05) iPM and iAc after 4 hr. Glycerol 5% had greater (p < .05) hPMM after 4 hr and iAc after 2 hr than at 0 hr. SOD 100 U ml-1had lower (p < .05) linearity and wobble compared to control group. No was observed differences to fertilisation rate (p < .05) among groups. In conclusion, glycerol 5% in Tris-egg yolk extender for 4 hr is suitable for the preservation of sperm kinetics and membrane integrity. CAT (50 and 100 U ml-1) or SOD (50-100 U ml-1) had no beneficial effects on sperm kinetics, plasma and acrosomal membrane integrity, mitochondrial activity or the capacity for IVF of frozen-thawed spermatozoa from epididymis of Nelore bulls.
authors.affiliation: Department of Veterinary Medicine, Andrology Laboratory, Federal Rural University of Pernambuco, Recife, Brazil.
authors.forename: F C
authors.initials: FC
authors.lastname: Almeida
authors.affiliation: Biotechnology Laboratory in Animal Reproduction, Biotechnology Center, Federal University of Paraíba, João Pessoa, Brazil.
authors.forename: S V
authors.initials: SV
authors.lastname: Silva
authors.affiliation: Department of Veterinary Medicine, Andrology Laboratory, Federal Rural University of Pernambuco, Recife, Brazil.
authors.forename: H M
authors.initials: HM
authors.lastname: Souza
authors.affiliation: Department of Veterinary Medicine, Andrology Laboratory, Federal Rural University of Pernambuco, Recife, Brazil.
authors.forename: W A
authors.initials: WA
authors.lastname: Gomes
authors.affiliation: Department of Veterinary Medicine, Andrology Laboratory, Federal Rural University of Pernambuco, Recife, Brazil.
authors.forename: J A C
authors.initials: JA
authors.lastname: Lima Filho
authors.affiliation: Ciclo Veterinária, CENATTE Embriões, São Gonçalo dos Campos, Brazil.
authors.forename: A A
authors.initials: AA
authors.lastname: Wicke
authors.affiliation: Department of Veterinary Medicine, Andrology Laboratory, Federal Rural University of Pernambuco, Recife, Brazil.
authors.forename: A M
authors.initials: AM
authors.lastname: Batista
authors.affiliation: Department of Veterinary Medicine, Andrology Laboratory, Federal Rural University of Pernambuco, Recife, Brazil.
authors.forename: M M P
authors.initials: MM
authors.lastname: Guerra
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000494:pharmacology; D002417:Cattle; D015925:Cryopreservation / Q000662:veterinary; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004822:Epididymis / Q000166:cytology*; D005615:Freezing / Q000009:adverse effects; D005990:Glycerol / Q000494:pharmacology*; D007315:Insemination, Artificial / Q000662:veterinary*; D008297:Male; D012662:Semen Preservation / Q000662:veterinary*; D013048:Specimen Handling / Q000662:veterinary*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000494:pharmacology; D013997:Time Factors
chemical_list: D000975:Antioxidants; D003451:Cryoprotective Agents; D002374:Catalase; D013482:Superoxide Dismutase; D005990:Glycerol
keywords: bull; catalase; cryopreservation; spermatozoa; superoxide dismutase