title: Genetically Similar Isolates of Salmonella enterica Serotype Enteritidis Persistent in China for a Long-Term Period.
abstract: Salmonella enterica serotype Enteritidis (S. Enteritidis) is an important causative agent of nontyphoidal salmonellosis in human populations. In this study, we collected 72 S. Enteritidis strains from 2004 to 2014 in Ningbo, mid-east China. Of the 72 strains, we identified a dominant clone of 58 strains recovered from patient's feces (n = 48), blood (n = 1), pleural effusion (n = 1), chickens (n = 3), and dessert cakes (n = 5) by pulsed-field gel electrophoresis (PFGE) and variable-number of tandem repeat analysis (MLVA). The profile arrangements of MLVA were SE1-SE2-SE3-SE5-SE6-SE8-SE9: 4-4-3-11-10-1-3. These dominant strains were susceptible to ampicillin, chloramphenicol, tetracycline, ciprofloxacin, gentamicin, cefotaxime and trimethoprim-sulfamethoxazole, and resistant to nalidixic acid. Additionally, all isolates harboured virulence genes invA, sipA, sopE, and spvB when tested by PCR. Our results reveal that genetically similar S. Enteritidis strains which accounted for several outbreaks as well as blood infection and pleural cavity infection are prevalent in China for a long-term period. This situation calls for further attention in the prevention and control of foodborne disease caused by Salmonella species.
authors.affiliation: Dept. of Microbiology, Ningbo Municipal Centre for Disease Control and Prevention, Ningbo, Zhejiang, People's Republic of China.
authors.forename: Qifa
authors.initials: Q
authors.lastname: Song
authors.affiliation: Dept. of Microbiology, Ningbo Municipal Centre for Disease Control and Prevention, Ningbo, Zhejiang, People's Republic of China.
authors.forename: Xuanyi
authors.initials: X
authors.lastname: Shen
authors.affiliation: Dept. of Microbiology, Ningbo Municipal Centre for Disease Control and Prevention, Ningbo, Zhejiang, People's Republic of China.
authors.forename: Yuanbin
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Dept. of Microbiology, Ningbo Municipal Centre for Disease Control and Prevention, Ningbo, Zhejiang, People's Republic of China.
authors.forename: Danyang
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Dept. of Microbiology, Ningbo Municipal Centre for Disease Control and Prevention, Ningbo, Zhejiang, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Gao
mesh_terms: D000667:Ampicillin / Q000494:pharmacology; D000820:Animal Diseases / Q000382:microbiology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002645:Chickens / Q000382:microbiology; D002681:China / Q000453:epidemiology; D002701:Chloramphenicol / Q000494:pharmacology; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D005516:Food Microbiology; D005798:Genes, Bacterial; D005839:Gentamicins / Q000494:pharmacology; D006801:Humans; D008826:Microbial Sensitivity Tests; D012478:Salmonella Food Poisoning / Q000382:microbiology*; D012480:Salmonella Infections / Q000382:microbiology; D019779:Salmonella enterica; D012477:Salmonella enteritidis / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D065288:Serogroup; D013752:Tetracycline / Q000494:pharmacology; D014774:Virulence / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins; D002701:Chloramphenicol; D000667:Ampicillin; D013752:Tetracycline
keywords: Salmonella enterica serotype enteritidis; dominant clone; pulsed-field gel electrophoresis (PFGE); variable-number of tandem repeat analysis (MLVA)