title: Complementary Effects of Genetic Variations in LEPR on Body Composition and Soluble Leptin Receptor Concentration after 3-Month Lifestyle Intervention in Prepubertal Obese Children.
abstract: In obese individuals, weight loss might be affected by variants of the adipokine-encoding genes. We verified whether selected functional single nucleotide polymorphisms in LEP, LEPR and ADIPOQ are associated with changes in serum levels of the respective adipokines and weight loss in 100 prepubertal obese (SDS-BMI > 2) Caucasian children undergoing lifestyle intervention. Frequencies of the -2548G > A LEP, Q223R LEPR, K656N LEPR, -11377C > G and -11426A > G ADIPOQ polymorphisms were analyzed by restriction fragment length polymorphism. Serum adipokine and soluble leptin receptor (sOB-R) concentrations were measured using the ELISA method. Among the analyzed polymorphisms, only LEPR polymorphisms were associated with changes of SDS-BMI or sOB-R concentrations in children after therapy. Carriers of the wild-type K665N and at least one minor Q223R allele had the greatest likelihood of losing weight (OR = 5.09, p = 0.006), an increase in sOB-R (ptrend = 0.022) and decrease in SDS-BMI correlated with the decrease of fat mass (p < 0.001). In contrast, carrying of the wild-type Q223R and at least one minor K665N allele were associated with a decrease in sOB-R concentrations and a decrease in SDS-BMI correlated with a decrease in fat-free mass (p = 0.002). We suggest that the combination of different LEPR variants, not a single variant, might determine predisposition to weight loss in the prepubertal period.
authors.affiliation: Screening Department, Institute of Mother and Child, Kasprzaka 17a, Warsaw 01-211, Poland. joanna.gajewska@imid.med.pl.
authors.forename: Joanna
authors.initials: J
authors.lastname: Gajewska
authors.affiliation: Department of Human Epigenetics, Mossakowski Medical Research Center, Polish Academy of Sciences, Warsaw 02-106, Poland. kurylowiczala@gazeta.pl.
authors.forename: Alina
authors.initials: A
authors.lastname: Kuryłowicz
authors.affiliation: Department of Epidemiology and Biostatistics, Institute of Mother and Child, Warsaw 01-211, Poland. ewa.mierzejewska@imid.med.pl.
authors.forename: Ewa
authors.initials: E
authors.lastname: Mierzejewska
authors.affiliation: Screening Department, Institute of Mother and Child, Kasprzaka 17a, Warsaw 01-211, Poland. jagoda.ambroszkiewicz@imid.med.pl.
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Ambroszkiewicz
authors.affiliation: Screening Department, Institute of Mother and Child, Kasprzaka 17a, Warsaw 01-211, Poland. magdalena.chelchowska@imid.med.pl.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Chełchowska
authors.affiliation: Department of Nutrition, Institute of Mother and Child, Warsaw 01-211, Poland. halina.weker@imid.med.pl.
authors.forename: Halina
authors.initials: H
authors.lastname: Weker
authors.affiliation: Department of Human Epigenetics, Mossakowski Medical Research Center, Polish Academy of Sciences, Warsaw 02-106, Poland. mpuzianowska@wum.edu.pl.|Department of Geriatrics and Gerontology, Medical Center of Postgraduate Education, Warsaw 01-826, Poland. mpuzianowska@wum.edu.pl.
authors.forename: Monika
authors.initials: M
authors.lastname: Puzianowska-Kuźnicka
mesh_terms: D052242:Adiponectin / Q000097:blood / Q000235:genetics / Q000378:metabolism; D050154:Adiposity; D000483:Alleles; D015992:Body Mass Index; D002648:Child; D002675:Child, Preschool; D003131:Combined Modality Therapy; D000072001:Diet, Healthy; D004038:Diet, Reducing; D015444:Exercise; D005260:Female; D056726:Genetic Association Studies; D000070497:Healthy Lifestyle; D006579:Heterozygote; D006801:Humans; D020738:Leptin / Q000097:blood / Q000235:genetics / Q000378:metabolism; D008297:Male; D063766:Pediatric Obesity / Q000178:diet therapy / Q000235:genetics / Q000378:metabolism / Q000628:therapy*; D020641:Polymorphism, Single Nucleotide; D054411:Receptors, Leptin / Q000097:blood / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012995:Solubility; D015431:Weight Loss
chemical_list: C499918:ADIPOQ protein, human; D052242:Adiponectin; C515448:LEPR protein, human; D020738:Leptin; D054411:Receptors, Leptin
keywords: adipokines; leptin receptor; polymorphisms; prepubertal period; weight loss