title: Co-culture of Gastric Organoids and Immortalized Stomach Mesenchymal Cells.
abstract: Three-dimensional primary epithelial-derived gastric organoids have recently been established as an important tool to study gastric development, physiology, and disease. Specifically, mouse-derived fundic gastric organoids (mFGOs) co-cultured with Immortalized Stomach Mesenchymal Cells (ISMCs) reflect expression patterns of mature fundic cell types seen in vivo, thus allowing for long-term in vitro studies of gastric epithelial cell physiology, regeneration, and bacterial-host interactions. Here, we describe the development and culture of mFGOs, co-cultured with ISMCs.
authors.affiliation: Department of Molecular and Cellular Physiology, University of Cincinnati College of Medicine, 231 Albert B. Sabin Way, Room 4255 MSB, Cincinnati, OH, 45267-0576, USA.
authors.forename: Nina
authors.initials: N
authors.lastname: Bertaux-Skeirik
authors.affiliation: Department of Molecular and Cellular Physiology, University of Cincinnati College of Medicine, 231 Albert B. Sabin Way, Room 4255 MSB, Cincinnati, OH, 45267-0576, USA.
authors.forename: Jomaris
authors.initials: J
authors.lastname: Centeno
authors.affiliation: Department of Gastroenterology and Hepatology, The First Affiliated Hospital, Sun Yatsen University, Guangzhou, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Feng
authors.affiliation: Division of Gastroenterology, Children's Hospital Los Angeles, Los Angeles, CA, USA.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Schumacher
authors.affiliation: Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, USA.|Department of Medicine, Harvard Medical School, Boston, MA, USA.
authors.forename: Ramesh A
authors.initials: RA
authors.lastname: Shivdasani
authors.affiliation: Department of Molecular and Cellular Physiology, University of Cincinnati College of Medicine, 231 Albert B. Sabin Way, Room 4255 MSB, Cincinnati, OH, 45267-0576, USA. yana.zavros@uc.edu.
authors.forename: Yana
authors.initials: Y
authors.lastname: Zavros
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D005753:Gastric Mucosa / Q000166:cytology* / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D009940:Organoids / Q000166:cytology* / Q000378:metabolism
chemical_list: D015415:Biomarkers
keywords: Co-culture; Immortalized stomach mesenchymal cells; Mouse fundic gastric organoids