title: Electro-deposited poly-luminol molecularly imprinted polymer coating on carboxyl graphene for stir bar sorptive extraction of estrogens in milk.
abstract: Electrochemical polymerization of luminol molecularly imprinted polymer on carboxyl graphene (MIP/CG) was developed as stir bar sorptive extraction (SBSE) coating for selective pre-concentration and specific recognition of bisphenol A (BPA), hexoestrol and diethylstilbestrol in milk samples. Luminol was employed as monomer and BPA as the template to prepare MIP under 0-0.6V electro-polymerization. Carboxyl graphene was modified on pencil lead as the substrate to increase extraction capacity. The preparation and extraction conditions affecting the extraction efficiency were optimized. Under the optimized conditions, a good linearity of three estrogens was obtained in the range of 4-1000ngmL(-1). The average recoveries at the three spiked levels of the three estrogens ranged from 83.4% to 96.3% with the relative standard deviations (RSD)≤7.1%. The limits of detection were in the range of 0.36-1.09ngmL(-1). The developed method with low cost, high selectivity and good reproducibility can be potentially applied for determining trace estrogens in complex food samples.
authors.affiliation: Faculty of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Liu
authors.affiliation: Faculty of Marine Science, Ningbo University, Ningbo 315211, China.
authors.forename: Li
authors.initials: L
authors.lastname: Qiao
authors.affiliation: Faculty of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, China. Electronic address: ganning@nbu.edu.cn.
authors.forename: Ning
authors.initials: N
authors.lastname: Gan
authors.affiliation: Faculty of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, China.
authors.forename: Saichai
authors.initials: S
authors.lastname: Lin
authors.affiliation: Faculty of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Faculty of Marine Science, Ningbo University, Ningbo 315211, China.
authors.forename: Futao
authors.initials: F
authors.lastname: Hu
authors.affiliation: Faculty of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, China.
authors.forename: Jiayu
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing University of Finance and Economics, Nanjing 210007, China.
authors.forename: Yinji
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000327:Adsorption; D000818:Animals; D001559:Benzhydryl Compounds / Q000302:isolation & purification; D004054:Diethylstilbestrol / Q000302:isolation & purification; D004595:Electroplating / Q000379:methods; D004968:Estrogens, Non-Steroidal / Q000302:isolation & purification*; D005504:Food Analysis / Q000379:methods*; D006108:Graphite / Q000737:chemistry; D006589:Hexestrol / Q000302:isolation & purification; D057230:Limit of Detection; D008165:Luminol / Q000737:chemistry*; D008892:Milk / Q000737:chemistry*; D054802:Molecular Imprinting / Q000379:methods*; D010636:Phenols / Q000302:isolation & purification; D058105:Polymerization; D011108:Polymers / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000379:methods; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004054:Diethylstilbestrol; D004968:Estrogens, Non-Steroidal; D006108:Graphite; D006589:Hexestrol; D008165:Luminol; D010636:Phenols; D011108:Polymers; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Electrochemical polymerization; Estrogens; Molecularly imprinted polymer; Polyluminol; Stir bar sorptive extraction