title: Applications of Fourier Transform Ion Cyclotron Resonance (FT-ICR) and Orbitrap Based High Resolution Mass Spectrometry in Metabolomics and Lipidomics.
abstract: Metabolomics, along with other "omics" approaches, is rapidly becoming one of the major approaches aimed at understanding the organization and dynamics of metabolic networks. Mass spectrometry is often a technique of choice for metabolomics studies due to its high sensitivity, reproducibility and wide dynamic range. High resolution mass spectrometry (HRMS) is a widely practiced technique in analytical and bioanalytical sciences. It offers exceptionally high resolution and the highest degree of structural confirmation. Many metabolomics studies have been conducted using HRMS over the past decade. In this review, we will explore the latest developments in Fourier transform mass spectrometry (FTMS) and Orbitrap based metabolomics technology, its advantages and drawbacks for using in metabolomics and lipidomics studies, and development of novel approaches for processing HRMS data.
authors.affiliation: Department of Biological Sciences, College of Arts and Sciences, University of North Texas, Denton, TX 76203, USA. manoj.ghaste@unt.edu.
authors.forename: Manoj
authors.initials: M
authors.lastname: Ghaste
authors.affiliation: HighChem, Bratislava 81104, Slovakia. robert.mistrik@highchem.com.
authors.forename: Robert
authors.initials: R
authors.lastname: Mistrik
authors.affiliation: Department of Biological Sciences, College of Arts and Sciences, University of North Texas, Denton, TX 76203, USA. shulaev@unt.edu.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Shulaev
mesh_terms: D017357:Cyclotrons; D005583:Fourier Analysis; D050356:Lipid Metabolism; D013058:Mass Spectrometry / Q000379:methods*; D055432:Metabolomics / Q000379:methods*
keywords: FT-ICR-MS; FTMS; Orbitrap-MS; high resolution mass spectrometry; lipidomics; metabolomics; metabolomics data analysis