title: Chronic Myeloid Leukemia Blood Inflicted Injury in Cord Derived Wharton's Jelly Mesenchymal Stem Cells.
abstract: OBJECTIVE
  To determine the effects of blood from CML patients on human umbilical cord derived Wharton's jelly mesenchymal stem cells (WJMSCs) for evaluation of their therapeutic potential.
  
  
  METHODS
  An experimental study.
  
  
  METHODS
  Centre for Research in Molecular Medicine, University of Lahore, from September 2013 to December 2014.
  
  
  METHODS
  Possible behavior of WJMSCs in CMLpatients was assessed by culturing these cells in their plasma. WJMSCs at passage 3 were cultured in plasma isolated from 9 CMLpatients as well as 9 normal subjects. Effects on cell viability, proliferation, LDH release, paracrine factors (p38 and p53) and oxidative stress were evaluated.
  
  
  RESULTS
  WJMSCs cultured in plasma of CML patients showed decreased viability, slow proliferation, high LDH release, high expression of p38 and p53 and a high oxidative stress compared to normal subjects.
  
  
  CONCLUSIONS
  Stressed environment of CML patients' blood/plasma induced injury to WJMSCs as well as reduced their viability. Effectiveness of these cells for therapeutics of CML is, therefore, likely to be reduced.
authors.affiliation: IMBB, The University of Lahore, Defence Road Campus, Lahore.
authors.forename: Nadia
authors.initials: N
authors.lastname: Wajid
authors.affiliation: IMBB, The University of Lahore, Defence Road Campus, Lahore.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Ali
authors.affiliation: IMBB, The University of Lahore, Defence Road Campus, Lahore.
authors.forename: Sara
authors.initials: S
authors.lastname: Javed
authors.affiliation: IMBB, The University of Lahore, Defence Road Campus, Lahore.
authors.forename: Fatima
authors.initials: F
authors.lastname: Ali
authors.affiliation: IMBB, The University of Lahore, Defence Road Campus, Lahore.
authors.forename: Sanam Saiqa
authors.initials: SS
authors.lastname: Anwar
mesh_terms: D002454:Cell Differentiation; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000628:therapy*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D014470:Umbilical Cord / Q000166:cytology*; D059631:Wharton Jelly / Q000166:cytology / Q000378:metabolism*; D014947:Wounds and Injuries / Q000473:pathology / Q000601:surgery*