title: Harvesting Human Prostate Tissue Material and Culturing Primary Prostate Epithelial Cells.
abstract: In order to fully explore the biology of a complex solid tumor such as prostate cancer, it is desirable to work with patient tissue. Only by working with cells from a tissue can we take into account patient variability and tumor heterogeneity. Cell lines have long been regarded as the workhorse of cancer research and it could be argued that they are of most use when considered within a panel of cell lines, thus taking into account specified mutations and variations in phenotype between different cell lines. However, often very different results are obtained when comparing cell lines to primary cells cultured from tissue. It stands to reason that cells cultured from patient tissue represents a close-to-patient model that should and does produce clinically relevant data. This chapter aims to illustrate the methods of processing, storing and culturing cells from prostate tissue, with a description of potential uses.
authors.affiliation: YCR Cancer Research Unit, Department of Biology, University of York, Heslington, North Yorkshire, YO10 5DD, UK. fiona.frame@york.ac.uk.
authors.forename: Fiona M
authors.initials: FM
authors.lastname: Frame
authors.affiliation: YCR Cancer Research Unit, Department of Biology, University of York, Heslington, North Yorkshire, YO10 5DD, UK.
authors.forename: Davide
authors.initials: D
authors.lastname: Pellacani
authors.affiliation: YCR Cancer Research Unit, Department of Biology, University of York, Heslington, North Yorkshire, YO10 5DD, UK.
authors.forename: Anne T
authors.initials: AT
authors.lastname: Collins
authors.affiliation: YCR Cancer Research Unit, Department of Biology, University of York, Heslington, North Yorkshire, YO10 5DD, UK. n.j.maitland@york.ac.uk.
authors.forename: Norman J
authors.initials: NJ
authors.lastname: Maitland
mesh_terms: D004847:Epithelial Cells / Q000166:cytology* / Q000473:pathology; D006801:Humans; D008297:Male; D061251:Primary Cell Culture / Q000379:methods*; D011467:Prostate / Q000166:cytology* / Q000473:pathology; D011471:Prostatic Neoplasms / Q000473:pathology*; D017154:Stromal Cells / Q000166:cytology* / Q000473:pathology; D020858:Tissue and Organ Harvesting / Q000379:methods*; D014407:Tumor Cells, Cultured
keywords: Benign prostatic hyperplasia; Cancer; Primary epithelial cells; Prostate; Tissue