title: Modeling and optimization of antibacterial activity of the chitosan-based hydrogel films using central composite design.
abstract: In the present study, hydrogel films composed of chitosan-poly(vinyl alcohol)-gelatin-thyme honey were successfully prepared by casting method, and their anti-bacterial properties were modeled and optimized. Antibacterial properties of the prepared films were analyzed by applying agar diffusion method. Staphylococcus aureus and Pseudomonas aeruginosa were tested as Gram-positive and Gram-negative bacteria, respectively. In order to obtain the composition of the film with maximum inhibition zone against both above-mentioned bacterial strains, the experiments were designed using response surface methodology based on five-level central composite design with four parameters, including concentrations of chitosan, poly(vinyl alcohol), gelatin, and honey. The results indicated that the prepared samples had good antibacterial activities against these two studied bacteria strains. Response surface method is conducted to develop mathematical models for process responses. Variance analysis on the experimental data shows that inhibition zone can be predicted effectively with quadratic models. In addition, swelling properties and rate of water vapor transmission of the prepared hydrogel films were studied. Due to the successful results, this hydrogel film has an excellent potential to be explored further as a wound healing material. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2544-2553, 2016.
authors.affiliation: School of Chemical and Petroleum Engineering, Shiraz University, Shiraz, Iran.
authors.forename: Behnaz
authors.initials: B
authors.lastname: Lahooti
authors.affiliation: School of Chemical and Petroleum Engineering, Shiraz University, Shiraz, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Khorram
authors.affiliation: School of Chemical and Petroleum Engineering, Shiraz University, Shiraz, Iran.
authors.forename: Gholamreza
authors.initials: G
authors.lastname: Karimi
authors.affiliation: Burn and Wound Healing Research Center, Division of Plastic and Reconstructive Surgery, Department of Surgery, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Aliakbar
authors.initials: A
authors.lastname: Mohammadi
authors.affiliation: Burn and Wound Healing Research Center, Department of Microbiology, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Amir
authors.initials: A
authors.lastname: Emami
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001458:Bandages; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology*; D005780:Gelatin / Q000737:chemistry / Q000494:pharmacology*; D006722:Honey / Q000032:analysis; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D011142:Polyvinyl Alcohol / Q000737:chemistry / Q000494:pharmacology*; D011552:Pseudomonas Infections / Q000188:drug therapy; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013203:Staphylococcal Infections / Q000188:drug therapy; D013211:Staphylococcus aureus / Q000187:drug effects; D046930:Thymus Plant / Q000737:chemistry; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D020100:Hydrogels; D005780:Gelatin; D011142:Polyvinyl Alcohol; D048271:Chitosan
keywords: gelatin; natural thyme honey; poly(vinyl alcohol); response surface methodology