title: Two different dengue virus strains in the Japanese epidemics of 2014.
abstract: In late August 2014, dengue cases were reported in Japan, and a total of 162 cases were confirmed. In the present study, the envelope (E) gene sequences of 12 specimens from the dengue patients were determined. A dengue virus serotype 1 (DENV1) strain (D1/Hu/Shizuoka/NIID181/2014), which was clearly different from the first reported strain (D1/Hu/Saitama/NIID100/2014), was identified, although the other 11 specimens showed the same nucleotide sequences as D1/Hu/Saitama/NIID100/2014. The E gene sequences of two different strains were compared with those of nine DENV1 strains of imported cases in Japan in 2014. Phylogenetic analysis based on the E gene sequences showed that the D1/Hu/Saitama/NIID100/2014 strain was closely related to a strain isolated from an imported case from Singapore. Although no strain closely related to D1/Hu/Shizuoka/NIID181/2014 was found in these imported strains, the strain was closely related to isolates in Thailand and Taiwan in 2009. These data indicate that the dengue cases in Japan were caused by two different dengue virus strains that entered Japan through different means.
authors.affiliation: Department of Virology I, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku, Tokyo, 162-8640, Japan.
authors.forename: Eri
authors.initials: E
authors.lastname: Nakayama
authors.affiliation: Department of Virology I, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku, Tokyo, 162-8640, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Kotaki
authors.affiliation: Department of Virology I, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku, Tokyo, 162-8640, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Tajima
authors.affiliation: Department of Infectious Disease, Saitama City Hospital, 2460 Mimuro, Midori-ku, Saitama-shi, Saitama, 336-8522, Japan.
authors.forename: Miki
authors.initials: M
authors.lastname: Kawada
authors.affiliation: Department of Internal Medicine, Saitama City Hospital, 2460 Mimuro, Midori-ku, Saitama-shi, Saitama, 336-8522, Japan.
authors.forename: Kuniharu
authors.initials: K
authors.lastname: Miura
authors.affiliation: Department of Infectious Diseases, Toshima Hospital, 33-1 Sakaecho, Itabashi-ku, Tokyo, 173-0015, Japan.
authors.forename: Aki
authors.initials: A
authors.lastname: Gemma
authors.affiliation: Department of Infectious Diseases, Toshima Hospital, 33-1 Sakaecho, Itabashi-ku, Tokyo, 173-0015, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Adachi
authors.affiliation: Pathogen Genomics Center, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku, Tokyo, 162-8640, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Sekizuka
authors.affiliation: Pathogen Genomics Center, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku, Tokyo, 162-8640, Japan.
authors.forename: Kengo
authors.initials: K
authors.lastname: Kato
authors.affiliation: Pathogen Genomics Center, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku, Tokyo, 162-8640, Japan.
authors.forename: Akifumi
authors.initials: A
authors.lastname: Yamashita
authors.affiliation: Department of Virology I, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku, Tokyo, 162-8640, Japan.
authors.forename: Meng Ling
authors.initials: ML
authors.lastname: Moi
authors.affiliation: Department of Virology I, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku, Tokyo, 162-8640, Japan.
authors.forename: Makiko
authors.initials: M
authors.lastname: Ikeda
authors.affiliation: Department of Virology I, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku, Tokyo, 162-8640, Japan.
authors.forename: Kazumi
authors.initials: K
authors.lastname: Yagasaki
authors.affiliation: Department of Virology I, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku, Tokyo, 162-8640, Japan.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Shibasaki
authors.affiliation: Department of Virology I, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku, Tokyo, 162-8640, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Saijo
authors.affiliation: Pathogen Genomics Center, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku, Tokyo, 162-8640, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Kuroda
authors.affiliation: Department of Virology I, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku, Tokyo, 162-8640, Japan. takasaki@nih.go.jp.
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Takasaki
mesh_terms: D001483:Base Sequence / Q000235:genetics; D003715:Dengue / Q000821:virology*; D003716:Dengue Virus / Q000145:classification* / Q000235:genetics*; D004196:Disease Outbreaks; D006801:Humans; D007564:Japan / Q000453:epidemiology; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D013624:Taiwan / Q000453:epidemiology; D013785:Thailand / Q000453:epidemiology
chemical_list: D012367:RNA, Viral
keywords: Aedes albopictus; Dengue; Dengue virus serotype 1; Envelope protein; Japan