title: λ Recombination and Recombineering.
abstract: The bacteriophage λ Red homologous recombination system has been studied over the past 50 years as a model system to define the mechanistic details of how organisms exchange DNA segments that share extended regions of homology. The λ Red system proved useful as a system to study because recombinants could be easily generated by co-infection of genetically marked phages. What emerged from these studies was the recognition that replication of phage DNA was required for substantial Red-promoted recombination in vivo, and the critical role that double-stranded DNA ends play in allowing the Red proteins access to the phage DNA chromosomes. In the past 16 years, however, the λ Red recombination system has gained a new notoriety. When expressed independently of other λ functions, the Red system is able to promote recombination of linear DNA containing limited regions of homology (∼50 bp) with the Escherichia coli chromosome, a process known as recombineering. This review explains how the Red system works during a phage infection, and how it is utilized to make chromosomal modifications of E. coli with such efficiency that it changed the nature and number of genetic manipulations possible, leading to advances in bacterial genomics, metabolic engineering, and eukaryotic genetics.
authors.affiliation: Department of Microbiology and Physiological Systems, University of Massachusetts Medical School, Worcester, MA 01605.
authors.forename: Kenan C
authors.initials: KC
authors.lastname: Murphy
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics* / Q000378:metabolism; D002875:Chromosomes; D004261:DNA Replication; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics / Q000821:virology; D005818:Genetic Engineering; D023281:Genomics; D010957:Plasmids; D011995:Recombination, Genetic
chemical_list: D004269:DNA, Bacterial