title: High-amplitude theta wave bursts characterizing narcoleptic mice and patients are also produced by histamine deficiency in mice.
abstract: Histamine and orexins are wake promoters released by hypothalamic neurons. The activity of histamine neurons is increased by orexin neurons. Recently, it has been shown that orexin deficiency entails high-amplitude theta wave bursts during rapid eye movement sleep and cataplexy in narcoleptic mice. The primary aim of this study was to assess whether histamine system is involved in high-amplitude theta wave burst generation during rapid eye movement sleep. The secondary aim was to assess the effects of combined histamine and orexin deficiency on high-amplitude theta wave bursts during rapid eye movement sleep in mice. Twelve histidine-decarboxylase knockout mice with congenital histamine deficiency, seven double mutant mice with combined deficiency of orexin neurons and histamine, and 11 wild-type control mice were studied with electrodes for sleep recordings and a telemetric blood pressure transducer. High-amplitude theta wave bursts during rapid eye movement sleep were detected in each of the histidine-decarboxylase knockout and double mutant mice, whereas only one burst was found in a wild-type control mouse. High-amplitude theta wave bursts occurred significantly more often and were significantly longer in double mutant than in histidine-decarboxylase knockout mice. In conclusion, it was demonstrated that, similarly to orexin, the chronic impairment of histamine entailed high-amplitude theta wave bursts during rapid eye movement sleep. The current data also suggested a synergistic role of orexin and histamine signalling on high-amplitude theta wave bursts during rapid eye movement sleep in mice.
authors.affiliation: Laboratory of Physiological Regulations in Sleeping Mice (PRISM), Department of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Bastianini
authors.affiliation: Laboratory of Physiological Regulations in Sleeping Mice (PRISM), Department of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy.
authors.forename: Viviana
authors.initials: V
authors.lastname: Lo Martire
authors.affiliation: Laboratory of Physiological Regulations in Sleeping Mice (PRISM), Department of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Berteotti
authors.affiliation: Laboratory of Physiological Regulations in Sleeping Mice (PRISM), Department of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Silvani
authors.affiliation: Applied Quantum Medical Engineering, Graduate School of Engineering, Tohoku University, Sendai, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ohtsu
authors.affiliation: Physiologie intégrée du système d'éveil, Centre de recherche en neurosciences de Lyon, INSERM U1028-CNRS UMR 5292 Faculté de Médecine, Université Claude Bernard, Lyon, France.
authors.forename: Jian-Sheng
authors.initials: JS
authors.lastname: Lin
authors.affiliation: Laboratory of Physiological Regulations in Sleeping Mice (PRISM), Department of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy. giovanna.zoccoli@unibo.it.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Zoccoli
mesh_terms: D000818:Animals; D001794:Blood Pressure; D002385:Cataplexy / Q000235:genetics / Q000503:physiopathology*; D006632:Histamine / Q000172:deficiency* / Q000378:metabolism; D006640:Histidine Decarboxylase / Q000172:deficiency / Q000235:genetics; D006801:Humans; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009154:Mutation; D009474:Neurons / Q000378:metabolism; D000068797:Orexins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism; D012895:Sleep, REM; D013826:Theta Rhythm
chemical_list: D000068797:Orexins; D006632:Histamine; D006640:Histidine Decarboxylase
keywords: electroencephalogram; hypocretin; paroxysms