title: A simple histochemical method for the identification of cytotrophoblasts in tissue sections.
abstract: A simple method for the demonstration of placental cytotrophoblast cells is described, utilising the affinity of the lectin from Bandeiraea simplicifolia-II (BSA-II) for intracellular amylase-sensitive glycogen and a protocol using biotinylated BSA-II followed by an avidin-peroxidase revealing system. In early pregnancy, cytotrophoblast cells in chorionic and anchoring villi are deeply stained and with ongoing differentiation the staining gradually decreases in intensity, suggesting that this lectin can be a useful marker for these cells.
authors.affiliation: Maternal and Fetal Health Research Centre, Institute of Human Development, University of Manchester, Manchester Academic Health Science Centre, St Mary's Hospital, Oxford Road, Manchester, M13 9WL, UK. Electronic address: carolyn.jones@manchester.ac.uk.
authors.forename: Carolyn J P
authors.initials: CJ
authors.lastname: Jones
authors.affiliation: Maternal and Fetal Health Research Centre, Institute of Human Development, University of Manchester, Manchester Academic Health Science Centre, St Mary's Hospital, Oxford Road, Manchester, M13 9WL, UK.
authors.forename: Saxon C
authors.initials: SC
authors.lastname: Whittle
authors.affiliation: Maternal and Fetal Health Research Centre, Institute of Human Development, University of Manchester, Manchester Academic Health Science Centre, St Mary's Hospital, Oxford Road, Manchester, M13 9WL, UK.
authors.forename: John D
authors.initials: JD
authors.lastname: Aplin
mesh_terms: D002824:Chorionic Villi / Q000378:metabolism; D005260:Female; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D010920:Placenta / Q000166:cytology* / Q000378:metabolism; D011247:Pregnancy; D013194:Staining and Labeling / Q000379:methods*; D014327:Trophoblasts / Q000166:cytology* / Q000378:metabolism
chemical_list: D037102:Lectins
keywords: Cytotrophoblast; Differentiation; Glycogen; Lectin; Placenta