title: Effect of macrophages on in vitro corrosion behavior of magnesium alloy.
abstract: The influence of cells on the corrosion behavior of biomedical magnesium alloy is an important but less studied topic, which is helpful for understanding the inconsistent corrosion rates between in vitro and in vivo experiments. In this work, macrophages were directly cultured on Mg-2.1Nd-0.2Zn-0.5Zr (wt %, abbreviated as JDBM) alloy surface for 72 or 168 hours. Macrophages retained good viability and the generation of reactive oxygen species (ROS) was greatly promoted on the alloy. Weight loss, Mg(2+) concentration, and cross-section observation results demonstrated that macrophages accelerated the in vitro corrosion of JDBM. The coverage of cell body did not affect the local thickness of corrosion product layer. The corrosion product layer had a porous inner Mg(OH)2 layer and a dense outer layer mainly composed of O, P, Mg, and Ca. The uniform acceleration of JDBM corrosion was attributed to the omnidirection diffusion of ROS from macrophages. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2476-2487, 2016.
authors.affiliation: National Engineering Research Center of Light Alloy Net Forming and State Key Laboratory of Metal Matrix Composite, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai, People's Republic of China.|Biomaterials Unit, International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science, Tsukuba, Japan.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Biomaterials Unit, International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science, Tsukuba, Japan.
authors.forename: Sachiko
authors.initials: S
authors.lastname: Hiromoto
authors.affiliation: Biomaterials Unit, International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science, Tsukuba, Japan.
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Yamazaki
authors.affiliation: National Engineering Research Center of Light Alloy Net Forming and State Key Laboratory of Metal Matrix Composite, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Jialin
authors.initials: J
authors.lastname: Niu
authors.affiliation: National Engineering Research Center of Light Alloy Net Forming and State Key Laboratory of Metal Matrix Composite, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Hua
authors.initials: H
authors.lastname: Huang
authors.affiliation: National Engineering Research Center of Light Alloy Net Forming and State Key Laboratory of Metal Matrix Composite, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Gaozhi
authors.initials: G
authors.lastname: Jia
authors.affiliation: Med-X Research Institute, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Li
authors.affiliation: National Engineering Research Center of Light Alloy Net Forming and State Key Laboratory of Metal Matrix Composite, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Wenjiang
authors.initials: W
authors.lastname: Ding
authors.affiliation: National Engineering Research Center of Light Alloy Net Forming and State Key Laboratory of Metal Matrix Composite, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai, People's Republic of China.
authors.forename: Guangyin
authors.initials: G
authors.lastname: Yuan
mesh_terms: D000497:Alloys / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D003343:Corrosion; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008274:Magnesium / Q000737:chemistry / Q000378:metabolism*; D008422:Materials Testing; D051379:Mice; D000067996:RAW 264.7 Cells; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000497:Alloys; D001672:Biocompatible Materials; D017382:Reactive Oxygen Species; D008274:Magnesium
keywords: biomedical magnesium alloy; in vitro corrosion; macrophage; reactive oxygen species