title: Identification of Preferentially Expressed Antigen of Melanoma as a Potential Tumor Suppressor in Lung Adenocarcinoma.
abstract: BACKGROUND Preferentially expressed antigen of melanoma (PRAME) is known as a tumor-associated antigen that is altered in a variety of malignancies, including lung cancer. However, the role of PRAME in lung cancer remains unclear. MATERIAL AND METHODS We analyzed the expression of PRAME in human lung adenocarcinomas and studied the function of PRAME using small interfering RNA (siRNA)-induced gene knockdown in lung cancer cell lines PC9 and A549. RESULTS We found that PRAME expression is down-regulated in lung adenocarcinomas. Knockdown of PRAME promoted proliferation and suppressed apoptosis of PC9 and A549 cells. CONCLUSIONS In line with its roles in controlling cell growth, RPAME regulates multiple critical cell-growth related genes, including IGF1R oncogene. IGF1R up-regulation contributes to increase of cell growth upon the knockdown of PRAME. Taken together, our results suggest that PRAME has inhibitory roles in lung cancer.
authors.affiliation: Department of Orthopedic Oncology, Changzheng Hospital, The Second Military Medical University, Shanghai, China (mainland).
authors.forename: Quan
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Department of Orthopedic Oncology, Changzheng Hospital, The Second Military Medical University, Shanghai, China (mainland).
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Orthopedic Oncology, Changzheng Hospital, The Second Military Medical University, Shanghai, China (mainland).
authors.forename: Zaijun
authors.initials: Z
authors.lastname: Lin
authors.affiliation: , The International Peace Maternity & Child Health Hospital of China Welfare Institute (IPMCH), Shanghai, China (mainland).
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.affiliation: Department of Orthopedic Oncology, Changzheng Hospital, The Second Military Medical University, Shanghai, China (mainland).
authors.forename: Shuai
authors.initials: S
authors.lastname: Han
authors.affiliation: Department of Orthopedic Oncology, Changzheng Hospital, The Second Military Medical University, Shanghai, China (mainland).
authors.forename: Chenglong
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Department of Orthopedic Oncology, Changzheng Hospital, The Second Military Medical University, Shanghai, China (mainland).
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.affiliation: , The International Peace Maternity & Child Health Hospital of China Welfare Institute (IPMCH), Shanghai, China (mainland).
authors.forename: Wenjiao
authors.initials: W
authors.lastname: Cao
authors.affiliation: Department of Orthopedic Oncology, Changzheng Hospital, The Second Military Medical University, Shanghai, China (mainland).
authors.forename: Xinghai
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Orthopedic Oncology, Changzheng Hospital, The Second Military Medical University, Shanghai, China (mainland).
authors.forename: Haifeng
authors.initials: H
authors.lastname: Wei
authors.affiliation: Department of Orthopedic Oncology, Changzheng Hospital, The Second Military Medical University, Shanghai, China (mainland).
authors.forename: Jianru
authors.initials: J
authors.lastname: Xiao
mesh_terms: D000072283:A549 Cells; D000230:Adenocarcinoma / Q000175:diagnosis / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D000077192:Adenocarcinoma of Lung; D000951:Antigens, Neoplasm / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D017209:Apoptosis / Q000502:physiology; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D016147:Genes, Tumor Suppressor; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000235:genetics; D017526:Receptor, IGF Type 1; D017451:Receptors, Somatomedin / Q000235:genetics / Q000276:immunology; D014162:Transfection; D015854:Up-Regulation
chemical_list: D000951:Antigens, Neoplasm; C000597236:IGF1R protein, human; C404944:PRAME protein, human; D034741:RNA, Small Interfering; D017451:Receptors, Somatomedin; D017526:Receptor, IGF Type 1