title: [Interlaboratory test: Isolation of Mycobacterium bovis from granulomatous lesions in bovine].
abstract: Mycobacterium bovis is the causative agent of bovine tuberculosis. The diagnostic laboratory confirmation is made through bacterial isolation. The aim of interlaboratory tests is to assess the performance of each participant in comparison with other of similar capacities. The test objective was to determine the efficiency of isolation of M. bovis. Four laboratories were part of the test and processed 25 blind tissue samples from granulomatous lesions and with previous M. bovis isolation. The laboratory that had the highest proportion of isolates was A (68%), followed by C (60%) and then B and D (both with 52%). The greatest concordance was observed between B-D and B-C laboratories (68%). The differences could be due to specific factors in each laboratory procedures. This type of interlaboratory tests highlights errors in the bacteriology and identifies critical points in the process to detect M. bovis accurately.
authors.affiliation: Instituto de Patobiología, CICVyA-INTA, Buenos Aires, Argentina.
authors.forename: Sergio
authors.initials: S
authors.lastname: Garbaccio
authors.affiliation: Facultad de Veterinaria, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Soledad
authors.initials: S
authors.lastname: Barandiaran
authors.affiliation: Secretaría de la Producción de Entre Ríos, Dirección General de Producción Animal, Paraná, Entre Ríos, Argentina.
authors.forename: Analía
authors.initials: A
authors.lastname: Fernandez
authors.affiliation: Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina.
authors.forename: Analía
authors.initials: A
authors.lastname: Macias
authors.affiliation: Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Magnano
authors.affiliation: Facultad de Veterinaria, Universidad de Buenos Aires, Buenos Aires, Argentina.
authors.forename: Marcela
authors.initials: M
authors.lastname: Martinez Vivot
authors.affiliation: Secretaría de la Producción de Entre Ríos, Dirección General de Producción Animal, Paraná, Entre Ríos, Argentina.
authors.forename: Maite
authors.initials: M
authors.lastname: Peyrú
authors.affiliation: Instituto de Biotecnología, CICVyA-INTA, Buenos Aires, Argentina. Electronic address: cataldi.angeladrian@inta.gob.ar.
authors.forename: Angel
authors.initials: A
authors.lastname: Cataldi
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques / Q000295:instrumentation; D002417:Cattle; D004203:Disinfection / Q000379:methods; D004866:Equipment Contamination; D007202:Indicators and Reagents; D059021:Laboratory Proficiency Testing; D008099:Liver / Q000382:microbiology; D008168:Lung / Q000382:microbiology; D008198:Lymph Nodes / Q000382:microbiology; D009163:Mycobacterium bovis / Q000302:isolation & purification*; D015203:Reproducibility of Results; D016037:Single-Blind Method; D013048:Specimen Handling / Q000379:methods; D014375:Tuberculoma / Q000382:microbiology / Q000662:veterinary*; D014380:Tuberculosis, Bovine / Q000382:microbiology*
chemical_list: D007202:Indicators and Reagents
keywords: Aislamiento; Bovine tuberculosis; Isolation; Mycobacterium bovis; Tuberculosis bovina