title: Use of anesthesia dramatically alters the oral glucose tolerance and insulin secretion in C57Bl/6 mice.
abstract: Evaluation of the impact of anesthesia on oral glucose tolerance in mice. Anesthesia is often used when performing OGTT in mice to avoid the stress of gavage and blood sampling, although anesthesia may influence gastrointestinal motility, blood glucose, and plasma insulin dynamics. C57Bl/6 mice were anesthetized using the following commonly used regimens: (1) hypnorm/midazolam repetitive or single injection; (2) ketamine/xylazine; (3) isoflurane; (4) pentobarbital; and (5) A saline injected, nonanesthetized group. Oral glucose was administered at time 0 min and blood glucose measured in the time frame -15 to +150 min. Plasma insulin concentration was measured at time 0 and 20 min. All four anesthetic regimens resulted in impaired glucose tolerance compared to saline/no anesthesia. (1) hypnorm/midazolam increased insulin concentrations and caused an altered glucose tolerance; (2) ketamine/xylazine lowered insulin responses and resulted in severe hyperglycemia throughout the experiment; (3) isoflurane did not only alter the insulin secretion but also resulted in severe hyperglycemia; (4) pentobarbital resulted in both increased insulin secretion and impaired glucose tolerance. All four anesthetic regimens altered the oral glucose tolerance, and we conclude that anesthesia should not be used when performing metabolic studies in mice.
authors.affiliation: Novo Nordisk Foundation Center for Basic Metabolic Research, Translational Metabolic Physiology and Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Johanne A
authors.initials: JA
authors.lastname: Windeløv
authors.affiliation: Novo Nordisk Foundation Center for Basic Metabolic Research, Translational Metabolic Physiology and Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark jensp@sund.ku.dk.
authors.forename: Jens
authors.initials: J
authors.lastname: Pedersen
authors.affiliation: Novo Nordisk Foundation Center for Basic Metabolic Research, Translational Metabolic Physiology and Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Jens J
authors.initials: JJ
authors.lastname: Holst
mesh_terms: D000777:Anesthetics; D000818:Animals; D001786:Blood Glucose / Q000032:analysis*; D002090:Butyrophenones; D004338:Drug Combinations; D005260:Female; D005283:Fentanyl; D018149:Glucose Intolerance / Q000139:chemically induced*; D005951:Glucose Tolerance Test; D007328:Insulin / Q000097:blood*; D007333:Insulin Resistance / Q000502:physiology*; D007530:Isoflurane; D007649:Ketamine; D051379:Mice; D008874:Midazolam; D010424:Pentobarbital; D014991:Xylazine
chemical_list: D000777:Anesthetics; D001786:Blood Glucose; D002090:Butyrophenones; D004338:Drug Combinations; D007328:Insulin; D014991:Xylazine; C001432:Hypnorm; D007649:Ketamine; D007530:Isoflurane; D010424:Pentobarbital; D008874:Midazolam; D005283:Fentanyl
keywords: Anesthesia; metabolic test; oral glucose tolerance test