title: Infectious Viral Quantification of Chikungunya Virus-Virus Plaque Assay.
abstract: The plaque assay is an essential method for quantification of infectious virus titer. Cells infected with virus particles are overlaid with a viscous substrate. A suitable incubation period results in the formation of plaques, which can be fixed and stained for visualization. Here, we describe a method for measuring Chikungunya virus (CHIKV) titers via virus plaque assays.
authors.affiliation: Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, 5 Science Drive 2, Singapore, 117597, Singapore.
authors.forename: Parveen
authors.initials: P
authors.lastname: Kaur
authors.affiliation: Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, 5 Science Drive 2, Singapore, 117597, Singapore.
authors.forename: Regina Ching Hua
authors.initials: RC
authors.lastname: Lee
authors.affiliation: Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, 5 Science Drive 2, Singapore, 117597, Singapore. justin_chu@nuhs.edu.sg.
authors.forename: Justin Jang Hann
authors.initials: JJ
authors.lastname: Chu
mesh_terms: D000818:Animals; D002460:Cell Line; D002646:Chikungunya virus / Q000254:growth & development*; D019562:Viral Load / Q000379:methods; D010948:Viral Plaque Assay / Q000379:methods*
keywords: Aquacide; Carboxymethyl cellulose; Chikungunya virus; Crystal violet; Plaque assays; Virus titration