title: Methyl-β-Cyclodextrin Impairs the Monocyte-Adhering Ability of Endothelial Cells by Down-Regulating Adhesion Molecules and Caveolae and Reorganizing the Actin Cytoskeleton.
abstract: Due to its powerful ability to deplete cholesterol from the plasma membrane of cells, methyl-β-cyclodextrin (MβCD) has been widely used as a putative research tool in cell biology. Recently, recruiting MβCD as an effective drug (e.g., antitumor drugs) has been developed. However, it remains unclear whether MβCD, when it enters the blood circulation as a drug, influences the functions of the endothelium, e.g., the adhesion of leukocytes to the endothelium. In this study, we found that MβCD can impair the adhesion of monocytes to the monolayer of endothelial cells by lowering the cell-surface adhesive force and expression of adhesion molecules and caveolae-related molecules on/in endothelial cells, and reorganizing the actin cytoskeleton of endothelial cells. The data imply that MβCD, when recruited as a drug, potentially helps to inhibit inflammation or initiation/progression of atherosclerosis since its important early step is the adhesion of circulating leukocytes (e.g., monocytes) to the endothelium.
authors.affiliation: Nanoscale Science and Technology Laboratory, Institute for Advanced Study, Nanchang University.
authors.forename: Meiying
authors.initials: M
authors.lastname: Ao
authors.forename: Li
authors.initials: L
authors.lastname: Wu
authors.forename: Xing
authors.initials: X
authors.lastname: Zhou
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects; D021941:Caveolae / Q000187:drug effects; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D009000:Monocytes / Q000187:drug effects* / Q000502:physiology; D047392:beta-Cyclodextrins / Q000494:pharmacology*
chemical_list: D047392:beta-Cyclodextrins; C108732:methyl-beta-cyclodextrin; D018799:Intercellular Adhesion Molecule-1