title: Antioxidant and Anti-Inflammatory Effects of Rhei Rhizoma and Coptidis Rhizoma Mixture on Reflux Esophagitis in Rats.
abstract: The purpose of this study was to investigate the antioxidant and anti-inflammatory effects of the combined extract of Rhei rhizoma and Coptidis rhizoma (RC-mix) in experimental model of acute reflux esophagitis. The antioxidant activity was assessed by in vitro 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. RC-mix was given at 100, 200, and 400 mg/kg body weight 2 h prior to induction of reflux esophagitis (RE). After 5 h, the effects of RC-mix treated rats were compared with those of normal and control rats. The representative flavonoid contents of RC-mix, such as sennoside A, epiberberine, coptisine, palmatine, and berberine, were detected using HPLC. The elevated esophageal mucosa damage was markedly ameliorated by RC-mix treatment in a dose-dependent manner. Furthermore, the administration of RC-mix reduced the increase of serum reactive oxygen species (ROS) and peroxynitrite (ONOO(-)). The improvement of superoxide dismutase (SOD) and heme oxygenase-1 (HO-1) levels were marked in the group given RC-mix. Moreover, the elevation of inflammatory mediators and cytokines by nuclear factor-kappa B (NF-κB) activation in control rats decreased by RC-mix pretreatment. These results indicate that RC-mix treatment reduces the pathological states of esophagitis via regulating NF-κB mediated inflammation related to oxidative stress.
authors.affiliation: Kyeoungbuk Institute for Regional Program Evaluation, Gyeongbuk TP, 27 Sampoong-ro, Gyeongsan, Gyeongsangbuk-do 38542, Republic of Korea.
authors.forename: O Jun
authors.initials: OJ
authors.lastname: Kwon
authors.affiliation: College of Korean Medicine, Daegu Haany University, Gyeongsan 38610, Republic of Korea.
authors.forename: Min Yeong
authors.initials: MY
authors.lastname: Kim
authors.affiliation: College of Korean Medicine, Daegu Haany University, Gyeongsan 38610, Republic of Korea.
authors.forename: Sung Ho
authors.initials: SH
authors.lastname: Shin
authors.affiliation: College of Korean Medicine, Daegu Haany University, Gyeongsan 38610, Republic of Korea.
authors.forename: Ah Reum
authors.initials: AR
authors.lastname: Lee
authors.affiliation: College of Korean Medicine, Daegu Haany University, Gyeongsan 38610, Republic of Korea.
authors.forename: Joo Young
authors.initials: JY
authors.lastname: Lee
authors.affiliation: College of Korean Medicine, Daegu Haany University, Gyeongsan 38610, Republic of Korea.
authors.forename: Bu-Il
authors.initials: BI
authors.lastname: Seo
authors.affiliation: College of Korean Medicine, Daegu Haany University, Gyeongsan 38610, Republic of Korea.
authors.forename: Mi-Rae
authors.initials: MR
authors.lastname: Shin
authors.affiliation: College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 41566, Republic of Korea.
authors.forename: Hyun Gyu
authors.initials: HG
authors.lastname: Choi
authors.affiliation: College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 41566, Republic of Korea.
authors.forename: Jeong Ah
authors.initials: JA
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Catholic University of Daegu, Gyeongsangbuk-do 38430, Republic of Korea.
authors.forename: Byung Sun
authors.initials: BS
authors.lastname: Min
authors.affiliation: Department of Food Science and Biotechnology, Kyungnam University, Gyeongsangnam-do 51767, Republic of Korea.
authors.forename: Gyo-Nam
authors.initials: GN
authors.lastname: Kim
authors.affiliation: Department of Food Science & Nutrition, Tongmyong University, Busan 48520, Republic of Korea.
authors.forename: Jeong Sook
authors.initials: JS
authors.lastname: Noh
authors.affiliation: College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of Korea.
authors.forename: Man Hee
authors.initials: MH
authors.lastname: Rhee
authors.affiliation: College of Korean Medicine, Daegu Haany University, Gyeongsan 38610, Republic of Korea.
authors.forename: Seong-Soo
authors.initials: SS
authors.lastname: Roh