title: Direct and Highly Selective Drug Optosensing in Real, Undiluted Biological Samples with Quantum-Dot-Labeled Hydrophilic Molecularly Imprinted Polymer Microparticles.
abstract: Quantum-dot (QD)-labeled hydrophilic molecularly imprinted polymer (MIP) microparticles were prepared for direct and highly selective optosensing of an antibiotic drug (i.e., tetracycline (Tc)) in pure bovine/goat milks and bovine/porcine serums. "Living" CdTe QD-SiO2 composite microparticles with alkyl bromide groups on their surfaces were first obtained via the one-pot sol-gel reaction, and they were subsequently grafted with a Tc-imprinted polymer layer and poly(glyceryl monomethacrylate) brushes via the successive surface-initiated atom transfer radical polymerizations. The resulting MIP microparticles with QD labeling and hydrophilic polymer brushes could function properly in biological samples and showed obvious template-binding-induced fluorescence quenching, which make them a useful fluorescent chemosensor with limits of detection down to 0.14 μM in complex biological media. Moreover, a facile and effective approach was developed based on a newly derived equation to eliminate the false positives of the fluorescent chemosensor and provide it with wider linear detection concentration ranges in comparison with those obtained using the generally adopted Stern-Volmer equation. Furthermore, the fluorescent MIP chemosensor was also successfully applied for directly, sensitively, selectively, and accurately quantifying Tc in biological media, and the average recoveries were in the range of 95%∼105% even when several other drugs and the fluorescently interfering chlortetracycline were present in the samples.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Functional Polymer Materials (Ministry of Education), Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), and College of Chemistry, Nankai University , Tianjin 300071, P. R. China.
authors.forename: Yaqiong
authors.initials: Y
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Functional Polymer Materials (Ministry of Education), Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), and College of Chemistry, Nankai University , Tianjin 300071, P. R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Niu
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Functional Polymer Materials (Ministry of Education), Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), and College of Chemistry, Nankai University , Tianjin 300071, P. R. China.
authors.forename: Huiqi
authors.initials: H
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D001774:Blood Chemical Analysis / Q000295:instrumentation / Q000379:methods*; D002417:Cattle; D005504:Food Analysis / Q000295:instrumentation / Q000379:methods*; D006041:Goats; D057927:Hydrophobic and Hydrophilic Interactions; D008892:Milk / Q000737:chemistry*; D054802:Molecular Imprinting; D011108:Polymers / Q000737:chemistry*; D045663:Quantum Dots / Q000737:chemistry*; D044967:Serum / Q000737:chemistry*; D012822:Silicon Dioxide; D013552:Swine
chemical_list: D000900:Anti-Bacterial Agents; D011108:Polymers; D012822:Silicon Dioxide
keywords: biological samples; chemosensor; drug detection; molecularly imprinted polymers; quantum dots; tetracycline