title: Design, modification and in vitro evaluation of pectin's bucco-adhesiveness.
abstract: It was the aim of this study to synthesize pectin (PEC) with sulfhydryl groups and evaluate its suitability in buccal application.
  
  
  
  Native PEC was chemically modified by covalent attachment of sulfhydryl-bearing cysteine (CYS). Stability assays in form of water uptake behavior and erosion study were performed. Additionally, mucoadhesive study on buccal mucosa was performed.
  
  
  
  Pectin-cysteine (PECCYS) was successfully synthesized as proved by IR and Ellman's assay exhibiting 436.59 ± 127.87 µmol thiol groups per gram polymer. Stability assay showed that PECCYS revealed a 2.27-fold improved water uptake and mucoadhesiveness augmented 3.75-fold in comparison to unmodified PEC.
  
  
  
  PECCYS might be a future suitable excipient for buccal adhesive application.
authors.affiliation: Department of Pharmaceutical Technology, Institute of Pharmacy, Center for Molecular Biosciences Innsbruck, University of Innsbruck, Austria.
authors.forename: Flavia
authors.initials: F
authors.lastname: Laffleur
authors.affiliation: Department of Pharmaceutical Technology, Institute of Pharmacy, Center for Molecular Biosciences Innsbruck, University of Innsbruck, Austria.
authors.forename: Martina
authors.initials: M
authors.lastname: Zilio
authors.affiliation: Department of Pharmaceutical Technology, Institute of Pharmacy, Center for Molecular Biosciences Innsbruck, University of Innsbruck, Austria.
authors.forename: Claudia
authors.initials: C
authors.lastname: Menzel
authors.affiliation: Department of Pharmaceutical Technology, Institute of Pharmacy, Center for Molecular Biosciences Innsbruck, University of Innsbruck, Austria.
authors.forename: Noemi
authors.initials: N
authors.lastname: Lupo
authors.affiliation: Institute of Analytical Chemistry & Radiochemistry, Center for Chemistry & Biomedicine (CCB), University of Innsbruck, Austria.
authors.forename: Matthias
authors.initials: M
authors.lastname: Schmutzler
mesh_terms: D000268:Adhesiveness; D000284:Administration, Oral; D003545:Cysteine / Q000737:chemistry*; D015195:Drug Design; D009061:Mouth Mucosa / Q000187:drug effects; D010368:Pectins / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D013438:Sulfhydryl Compounds; D010368:Pectins; D003545:Cysteine
keywords: adhesive; buccal; mucoadhesion; mucosa; pectin