title: Biohydrogen Production from Hydrolysates of Selected Tropical Biomass Wastes with Clostridium Butyricum.
abstract: Biohydrogen production has received widespread attention from researchers in industry and academic fields. Response surface methodology (RSM) was applied to evaluate the effects of several key variables in anaerobic fermentation of glucose with Clostridium butyrium, and achieved the highest production rate and yield of hydrogen. Highest H2 yield of 2.02 mol H2/mol-glucose was achieved from 24 h bottle fermentation of glucose at 35 °C, while the composition of medium was (g/L): 15.66 glucose, 6.04 yeast extract, 4 tryptone, 3 K2HPO4, 3 KH2PO4, 0.05 L-cysteine, 0.05 MgSO4·7H2O, 0.1 MnSO4·H2O and 0.3 FeSO4·7H2O, which was very different from that for cell growth. Sugarcane bagasse and Jatropha hulls were selected as typical tropical biomass wastes to produce sugars via a two-step acid hydrolysis for hydrogen production. Under the optimized fermentation conditions, H2 yield (mol H2/mol-total reducing sugar) was 2.15 for glucose, 2.06 for bagasse hydrolysate and 1.95 for Jatropha hull hydrolysate in a 3L fermenter for 24 h at 35 °C, with H2 purity of 49.7-64.34%. The results provide useful information and basic data for practical use of tropical plant wastes to produce hydrogen.
authors.affiliation: University of Science and Technology of China, School of Life Science, 443 Huangshan Road, Hefei, Anhui Province 230022, China.|Chinese Academy of Sciences, Biomass Group, Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, 88 Xuefulu, Kunming, Yunnan Province 650223, China.
authors.lastname: Dan Jiang
authors.affiliation: University of Science and Technology of China, School of Life Science, 443 Huangshan Road, Hefei, Anhui Province 230022, China.|Chinese Academy of Sciences, Biomass Group, Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, 88 Xuefulu, Kunming, Yunnan Province 650223, China.|Biomas Group, College of Engineering, Nanjing Agricultural University, 40 Dianjiangtai Road, Nanjing, Jiangsu 210031, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Fang
authors.affiliation: Chinese Academy of Sciences, Biomass Group, Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, 88 Xuefulu, Kunming, Yunnan Province 650223, China.|Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43600 UKM, Bangi, Selangor, Malaysia.
authors.forename: Siew-Xian
authors.initials: SX
authors.lastname: Chin
authors.affiliation: Chinese Academy of Sciences, Biomass Group, Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, 88 Xuefulu, Kunming, Yunnan Province 650223, China.|School of Bioscience and Bioengineering, South China University of Technology, 382 Outer ring east road, Higher Education Mega Centre, Guangzhou, 510006, China.
authors.forename: Xiao-Fei
authors.initials: XF
authors.lastname: Tian
authors.affiliation: University of Science and Technology of China, School of Life Science, 443 Huangshan Road, Hefei, Anhui Province 230022, China.|Chinese Academy of Sciences, Biomass Group, Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, 88 Xuefulu, Kunming, Yunnan Province 650223, China.
authors.forename: Tong-Chao
authors.initials: TC
authors.lastname: Su
mesh_terms: D056804:Biofuels / Q000382:microbiology*; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D046972:Clostridium butyricum / Q000254:growth & development* / Q000378:metabolism; D005285:Fermentation; D006859:Hydrogen / Q000378:metabolism*
chemical_list: D056804:Biofuels; D006859:Hydrogen