title: Increased concentration of 8-hydroxy-2'-deoxyguanosine in follicular fluid of infertile women with endometriosis.
abstract: Impaired oocyte quality and oxidative stress might be involved in the pathogenesis of endometriosis-related infertility. To improve our understanding of the role of oxidative stress in this condition, we compare eight oxidative stress markers from each stage, including the simultaneous analysis of lipids, proteins and DNA damage, in the serum and follicular fluid of infertile women with endometriosis and infertile controls undergoing controlled ovarian stimulation for intracytoplasmic sperm injection. In total, 87 serum samples (43 with endometriosis, 44 controls) and 61 follicular fluid samples (29 with endometriosis, 32 controls) free of blood contamination upon visual inspection and presenting granulosa cells alone or granulosa cells plus a retrieved mature oocyte were collected on the day of oocyte retrieval. Total hydroperoxides, malondialdehyde, advanced oxidation protein products, glutathione, superoxide dismutase (SOD) and total antioxidant capacity (TAC) were determined by spectrophotometry, vitamin E by high-performance liquid chromatography and 8-hydroxy-2'-deoxyguanosine (8OHdG) by enzyme-linked immunosorbent assay. The endometriosis group showed higher serum concentrations of glutathione and SOD, lower serum concentrations of TAC and higher follicular concentrations of 8OHdG and vitamin E compared with infertile controls. These data indicate both systemic and follicular oxidative stress in infertile patients with endometriosis. For the first time, we demonstrate the presence of oxidative DNA damage, represented by higher 8OHdG concentrations in the follicular microenvironment of these patients, possibly related to compromised oocyte quality and associated with the pathogenesis of endometriosis-related infertility.
authors.affiliation: Human Reproduction Division, Department of Obstetrics and Gynecology, Ribeirão Preto School of Medicine, University of São Paulo, Avenida Bandeirantes, 3900 Monte Alegre, Ribeirao Preto, SP, Brazil, CEP: 14049-900. michi.dabroi@gmail.com.
authors.forename: Michele G
authors.initials: MG
authors.lastname: Da Broi
authors.affiliation: Human Reproduction Division, Department of Obstetrics and Gynecology, Ribeirão Preto School of Medicine, University of São Paulo, Avenida Bandeirantes, 3900 Monte Alegre, Ribeirao Preto, SP, Brazil, CEP: 14049-900.
authors.forename: Felipe O
authors.initials: FO
authors.lastname: de Albuquerque
authors.affiliation: Human Reproduction Division, Department of Obstetrics and Gynecology, Ribeirão Preto School of Medicine, University of São Paulo, Avenida Bandeirantes, 3900 Monte Alegre, Ribeirao Preto, SP, Brazil, CEP: 14049-900.
authors.forename: Aline Z
authors.initials: AZ
authors.lastname: de Andrade
authors.affiliation: Human Reproduction Division, Department of Obstetrics and Gynecology, Ribeirão Preto School of Medicine, University of São Paulo, Avenida Bandeirantes, 3900 Monte Alegre, Ribeirao Preto, SP, Brazil, CEP: 14049-900.
authors.forename: Rafaela L
authors.initials: RL
authors.lastname: Cardoso
authors.affiliation: Nutrition and Metabolism Laboratory, Department of Internal Medicine, Ribeirão Preto School of Medicine, University of Sao Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Alceu A
authors.initials: AA
authors.lastname: Jordão Junior
authors.affiliation: Human Reproduction Division, Department of Obstetrics and Gynecology, Ribeirão Preto School of Medicine, University of São Paulo, Avenida Bandeirantes, 3900 Monte Alegre, Ribeirao Preto, SP, Brazil, CEP: 14049-900.
authors.forename: Paula A
authors.initials: PA
authors.lastname: Navarro
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D004715:Endometriosis / Q000097:blood / Q000150:complications / Q000378:metabolism*; D005260:Female; D015571:Follicular Fluid / Q000378:metabolism*; D006801:Humans; D007247:Infertility, Female / Q000097:blood / Q000150:complications / Q000378:metabolism*; D054315:Oocyte Retrieval; D018384:Oxidative Stress
chemical_list: D015415:Biomarkers; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine
keywords: 8-hydroxy-2′-deoxyguanosine; Endometriosis; Follicular fluid; Infertility; Oxidative stress