title: Using Multiple Whole-Cell Recordings to Study Spike-Timing-Dependent Plasticity in Acute Neocortical Slices.
abstract: This protocol provides a method for quadruple whole-cell recording to study synaptic plasticity of neocortical connections, with a special focus on spike-timing-dependent plasticity (STDP). It also describes how to morphologically identify recorded cells from two-photon laser-scanning microscopy (2PLSM) stacks.
authors.affiliation: Centre for Research in Neuroscience, Department of Neurology and Neurosurgery, The Research Institute of the McGill University Health Centre, Montréal General Hospital, Montréal, Québec H3G 1A4, Canada Integrated Program in Neuroscience, McGill University, 3801 University Street, Montréal, Quebec H3A 2B4, Canada.
authors.forename: Txomin
authors.initials: T
authors.lastname: Lalanne
authors.affiliation: Centre for Research in Neuroscience, Department of Neurology and Neurosurgery, The Research Institute of the McGill University Health Centre, Montréal General Hospital, Montréal, Québec H3G 1A4, Canada.
authors.forename: Therese
authors.initials: T
authors.lastname: Abrahamsson
authors.affiliation: Centre for Research in Neuroscience, Department of Neurology and Neurosurgery, The Research Institute of the McGill University Health Centre, Montréal General Hospital, Montréal, Québec H3G 1A4, Canada.
authors.forename: P Jesper
authors.initials: PJ
authors.lastname: Sjöström
mesh_terms: D000818:Animals; D066298:In Vitro Techniques / Q000379:methods*; D019579:Neocortex / Q000502:physiology*; D009473:Neuronal Plasticity; D018408:Patch-Clamp Techniques / Q000379:methods*; D012377:Rodentia