title: Role of porcine serum haptoglobin in the host-parasite relationship of Taenia solium cysticercosis.
abstract: Human and porcine cysticercosis is a parasitic disease caused by the larval stage (cysts) of the tapeworm Taenia solium. Cysts may live in several host tissues such as skeletal muscle or brain. We have previously described the presence of host haptoglobin (Hp) and hemoglobin (Hb) in different protein extracts of the T. solium cysts. Here, we report the binding of host Hp and Hb to a number of cyst proteins, evaluated through measuring electrophoretic and light absorbance changes. In the sera obtained from 18 cysticercotic pigs, Hp-Hb complexes were abundant, whereas free Hp was undetectable. In contrast, in the sera from non 18 cysticercotic pigs, Hp-Hb and free Hp were found. In the soluble protein fraction of cysts tissue, free Hp was detected showing a considerable Hb-binding ability, whereas in the vesicular fluid, Hp is mainly bound to Hb. Interestingly, assays carried out with the insoluble fraction of T. solium cysts tissue, showed binding of Hp and Hp-Hb in a saturable way, suggesting the existence of specific interactions. Our results suggested that the parasite can take advantage of the uptaken host Hp and Hb, either free or in complexes, as a source of iron or as a way to modulate the inflammatory response surrounding the T. solium cysts.
authors.affiliation: Dept. of Immunology, Institute for Biomedical Research, Universidad Nacional Autónoma de México, México D.F. 04510, Mexico; Instituto Nacional de Medicina Genómica, Periférico Sur No. 4809, Col. Arenal Tepepan, Delegación Tlalpan, México, D.F. C.P. 14610, Mexico.
authors.forename: José
authors.initials: J
authors.lastname: Navarrete-Perea
authors.affiliation: Instituto de Química, Universidad Nacional Autónoma de México, México D.F. 04510, Mexico.
authors.forename: Yanis
authors.initials: Y
authors.lastname: Toledano-Magaña
authors.affiliation: Dept. of Immunology, Institute for Biomedical Research, Universidad Nacional Autónoma de México, México D.F. 04510, Mexico.
authors.forename: Patricia
authors.initials: P
authors.lastname: De la Torre
authors.affiliation: Dept. of Immunology, Institute for Biomedical Research, Universidad Nacional Autónoma de México, México D.F. 04510, Mexico.
authors.forename: Edda
authors.initials: E
authors.lastname: Sciutto
authors.affiliation: Dept. of Immunology, Institute for Biomedical Research, Universidad Nacional Autónoma de México, México D.F. 04510, Mexico.
authors.forename: Raúl José
authors.initials: RJ
authors.lastname: Bobes
authors.affiliation: Instituto Nacional de Medicina Genómica, Periférico Sur No. 4809, Col. Arenal Tepepan, Delegación Tlalpan, México, D.F. C.P. 14610, Mexico; Instituto de Biotecnología, Universidad Nacional Autónoma de México, Avenida Universidad 2001, Cuernavaca, Morelos, C.P. 62210, Mexico.
authors.forename: Xavier
authors.initials: X
authors.lastname: Soberón
authors.affiliation: Dept. of Immunology, Institute for Biomedical Research, Universidad Nacional Autónoma de México, México D.F. 04510, Mexico. Electronic address: laclette@biomedicas.unam.mx.
authors.forename: Juan Pedro
authors.initials: JP
authors.lastname: Laclette
mesh_terms: D000818:Animals; D003551:Cysticercosis / Q000097:blood / Q000469:parasitology / Q000662:veterinary*; D006242:Haptoglobins / Q000032:analysis* / Q000378:metabolism; D006454:Hemoglobins / Q000032:analysis / Q000378:metabolism; D006790:Host-Parasite Interactions; D006801:Humans; D018482:Muscle, Skeletal / Q000469:parasitology; D011485:Protein Binding; D013552:Swine; D013553:Swine Diseases / Q000097:blood / Q000276:immunology* / Q000469:parasitology*; D041201:Taenia solium / Q000502:physiology*
chemical_list: D006242:Haptoglobins; D006454:Hemoglobins; C035492:haptoglobin-hemoglobin complex
keywords: Haptoglobin; Haptoglobin-hemoglobin complexes; Hemoglobin; Host-parasite relationship; Iron source; Nutritional immunity