title: Oxidative stress biomarkers in endometrial secretions: A comparison between successful and unsuccessful in vitro fertilization cycles.
abstract: A potential role of oxidative stress has been implicated in the outcome of various steps of assisted reproductive technology (ART). In a prospective cohort study, a total of 100 patients undergoing IVF/ICSI procedure due to male factor infertility were recruited based on the inclusion criteria. In all patients, 1-2ml of endometrial secretions was aspirated prior to embryo transfer. The oxidative stress markers in endometrial secretions, including superoxide dismutase (SOD), catalase (CAT) activities, lipid peroxidation (LPO), total thiol groups (TTG), and total antioxidant power (TAP) were investigated and compared among study groups including term pregnancy, failed IVF cycle, and miscarriage. P<0.05 was considered statistically different. Of the 100 patients, 28 cases (28%) resulted in ongoing pregnancy (biochemical pregnancy followed by clinical pregnancy), 11 cases (11%) resulted in miscarriage, and 61 cases (61%), resulted in failed IVF cycle. SOD, LPO, CAT, and TAP levels in the endometrial secretions of the three groups were statistically different (P-value <0.01, <0.001, <0.001, and <0.001, respectively). TTG levels in endometrial secretion of three groups were not statistically different (P-value=0.837). Our results indicated that higher levels of antioxidants such as SOD, CAT, or TAP, and lower levels of oxidative stress markers such as LPO in the endometrial secretions were associated with successful IVF outcome.
authors.affiliation: Students' Research Committee, Hamadan University of Medical Sciences, Hamadan, ‎Iran.
authors.forename: Mohammad Ehsan
authors.initials: ME
authors.lastname: Rahiminejad
authors.affiliation: Department of Obstetrics and Gynecology, Baylor College of Medicine, Houston, TX, USA. Electronic address: amirh_moaddab@yahoo.com.
authors.forename: Amirhossein
authors.initials: A
authors.lastname: Moaddab
authors.affiliation: Students' Research Committee, Hamadan University of Medical Sciences, Hamadan, ‎Iran.
authors.forename: Maziar
authors.initials: M
authors.lastname: Ganji
authors.affiliation: Students' Research Committee, Hamadan University of Medical Sciences, Hamadan, ‎Iran.
authors.forename: Nika
authors.initials: N
authors.lastname: Eskandari
authors.affiliation: Department of Obstetrics and Gynecology, Baylor College of Medicine, Houston, TX, USA.
authors.forename: Mayel
authors.initials: M
authors.lastname: Yepez
authors.affiliation: Department of Obstetrics and Gynecology, Fatemieh Women's Hospital, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Soghra
authors.initials: S
authors.lastname: Rabiee
authors.affiliation: Department of Obstetrics and Gynecology, Baylor College of Medicine, Houston, TX, USA.
authors.forename: Meredith
authors.initials: M
authors.lastname: Wise
authors.affiliation: Department of Obstetrics and Gynecology, Baylor College of Medicine, Houston, TX, USA.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Ruano
authors.affiliation: Department of Toxicology and Pharmacology, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Akram
authors.initials: A
authors.lastname: Ranjbar
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism*; D015331:Cohort Studies; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007248:Infertility, Male / Q000175:diagnosis* / Q000628:therapy; D015227:Lipid Peroxidation; D008297:Male; D018384:Oxidative Stress; D011247:Pregnancy; D011256:Pregnancy Outcome; D011446:Prospective Studies; D013482:Superoxide Dismutase / Q000378:metabolism*; D055815:Young Adult
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D002374:Catalase; D013482:Superoxide Dismutase
keywords: Implantation; In vitro fertilization; Outcome; Oxidative stress; Pregnancy