title: New Cyclohexadepsipeptides from an Entomogenous Fungus Fusarium proliferatum and Their Cytotoxicity and Autophagy-Inducing Activity.
abstract: Five new cyclohexadepsipeptides termed as enniatins R - V (1 - 5) and seven known cyclohexadepsipeptides (6 - 12) were isolated from the solid culture of Fusarium proliferatum, a fungus isolated from the cadaver of an unidentified insect collected in Tibet. Their structures were elucidated by NMR and MS spectroscopic analysis. The X-ray single-crystal structure of 6 was reported for the first time. Enniatins R and S represented the first enniatins incorporating with an unusual 2,3-dihydroxy-isovaleric acid (Div) residue. The cytotoxicity and autophagy-inducing activities of 1 - 12 were evaluated in vitro. Beauvenniatin F (11) exhibited strong cytotoxicity against K562/A (adriamycin-resistant K562) with IC50 value of 3.78 μm, and also autophagy-inducing activity at the concentration of 20 μm in GFP-LC3 stable HeLa cells.
authors.affiliation: School of Medical Devices, Shenyang Pharmaceutical University, No. 103 Wenhua Road, Shenhe District, Shenyang, 110016, P. R. China.|State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichenxi Road, Chaoyang District, Beijing, 100101, P. R. China.
authors.forename: Jin
authors.initials: J
authors.lastname: Tian
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichenxi Road, Chaoyang District, Beijing, 100101, P. R. China.
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Han
authors.affiliation: School of Medical Devices, Shenyang Pharmaceutical University, No. 103 Wenhua Road, Shenhe District, Shenyang, 110016, P. R. China. shxuwei8720@163.com.
authors.forename: Xue
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichenxi Road, Chaoyang District, Beijing, 100101, P. R. China.
authors.forename: Lu-Wei
authors.initials: LW
authors.lastname: He
authors.affiliation: College of Life Science, Shanxi University, No. 92 Wucheng Road, Xiaodian District, Taiyuan, 03006, P. R. China.
authors.forename: Yong-Jie
authors.initials: YJ
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichenxi Road, Chaoyang District, Beijing, 100101, P. R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Bao
authors.affiliation: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichenxi Road, Chaoyang District, Beijing, 100101, P. R. China. liuhw@im.ac.cn.
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Liu
mesh_terms: D000970:Antineoplastic Agents; D001343:Autophagy / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D018360:Crystallography, X-Ray; D047630:Depsipeptides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005670:Fusarium / Q000737:chemistry*; D006801:Humans; D008958:Models, Molecular; D008968:Molecular Conformation; D013329:Structure-Activity Relationship
chemical_list: D000970:Antineoplastic Agents; D047630:Depsipeptides
keywords: Autophagy-inducing activity; Cyclohexadepsipeptides; Cytotoxicity; Fusarium proliferatum