title: Pathogenic characteristics of a novel triple-reasserted H1N2 swine influenza virus.
abstract: A novel triple reasserted H1N2 virus A/swine/Shanghai/1/2007 (SH07) was isolated from nasal swabs of weaned pig showing clinical symptoms of coughing and sneezing. To explore the virus characteristics, mice, chickens and pigs were selected for pathogenicity study. Pigs inoculated intranasally with 10(6) TCID50 SH07 showed clinical symptoms with coughing and sneezing, but no death. The virus nuclear acid was detected in many tissues using real-time PCR, which was mainly distributed in respiratory system particularly in the lungs. The virus was low-pathogenic to chickens with 10(6) TCID50 dose inoculation either via intramuscular or intranasal routes. However virus nuclear acid detection and virus isolation confirmed that the virus can also be found in nasal and rectum. When virus was inoculated into mice by intramuscular or intranasal routes we observed 100% and 80% lethality respectively. The third generation of samples passaged on MDCK cell were SIV positive in indirect immunofluorescence assay (IFA) using antiserum against H1N2 SIV. Furthermore, the lungs of mice showed obvious lesion with interstitial pneumonia. Data in our study suggest that SH07 is preferentially pathogenic to mammals rather than birds although it is a reasserting virus with the fragments from swine, human and avian origin.
authors.affiliation: Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, 201106, Shanghai, China; Municipal Key Laboratory of Agri-Genetics and Breedings, Shanghai 201106, China. Electronic address: huilil@163.com.
authors.forename: Huili
authors.initials: H
authors.lastname: Liu
authors.affiliation: Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, 201106, Shanghai, China; Municipal Key Laboratory of Agri-Genetics and Breedings, Shanghai 201106, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Tao
authors.affiliation: Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, 201106, Shanghai, China.
authors.forename: Pengchao
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, 201106, Shanghai, China; Municipal Key Laboratory of Agri-Genetics and Breedings, Shanghai 201106, China.
authors.forename: Xiuchen
authors.initials: X
authors.lastname: Yin
authors.affiliation: Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, 201106, Shanghai, China.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Ha
authors.affiliation: Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, 201106, Shanghai, China.
authors.forename: Chunling
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002645:Chickens; D004285:Dogs; D058507:Host Specificity / Q000276:immunology; D006801:Humans; D057221:Influenza A Virus, H1N2 Subtype / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D005585:Influenza in Birds / Q000276:immunology* / Q000821:virology; D008168:Lung / Q000276:immunology / Q000821:virology; D061985:Madin Darby Canine Kidney Cells; D051379:Mice; D008856:Microscopy, Fluorescence; D009296:Nasal Cavity / Q000276:immunology / Q000821:virology; D009976:Orthomyxoviridae Infections / Q000276:immunology* / Q000662:veterinary / Q000821:virology; D012007:Rectum / Q000276:immunology / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000821:virology; D014774:Virulence / Q000276:immunology
keywords: H1N2; Pathogenicity; Swine influenza virus