title: Shear stress regulates endothelial cell function through SRB1-eNOS signaling pathway.
abstract: OBJECTIVE
  The aim of this study was to explore whether transmembrane flow shear stress could regulate eNOS expression and mediate endothelial cell function via SR-B1 signaling transduction.
  
  
  METHODS
  Parallel-plate flow chamber was used to impose laminar shear stress and evaluate the effect of shear stress upon human umbilical vein endothelial cells (HUVECs). The expression of SR-B1 and eNOS at both RNA and protein levels was detected under different dynamic conditions. RNA interference and gene transfection were performed to overexpress and knock down the SRB1 to confirm the role of SR-B1-eNOS signaling pathway.
  
  
  RESULTS
  The expression levels of SR-B1 and eNOS were downregulated when HUVECs were treated with 4.2 dyne/cm(2) shear stress compared with those of the control group. However, the expression of SR-B1 and eNOS was upregulated as HUVECs exposed to 8.4 and 15 dyne/cm(2) shear stress. When exposed to 8.4 dyne/cm(2) , SR-B1 and eNOS expression was significantly higher compared with those of the other groups. When SR-B1 was knocked down through RNAi technique, the expression of eNOS was significantly downregulated than those of the other groups. While overexpression of SR-B1 could upregulate eNOS significantly.
  
  
  CONCLUSIONS
  Shear stress regulates endothelial cell function through SR-B1-eNOS signaling pathway. SR-B1 may play a pivotal role in the process of anti-atherosclerosis.
authors.affiliation: Department of Anesthesiology, Traditional Chinese Medicine Hospital of Luzhou Medical College, Luzhou, Sichuan Province, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: The Affiliated Hospital of Luzhou Medical College, Cardiothoracic Surgery, Luzhou, Sichuan Province, China.|Institute of Cardiovascular Research, Luzhou Medical College, Luzhou, Sichuan Province, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liao
authors.affiliation: Institute of Cardiovascular Research, Luzhou Medical College, Luzhou, Sichuan Province, China.|Key Laboratory of Medical Electrophysiology, Ministry of Education of China, Luzhou, Sichuan Province, China.
authors.forename: Miaoling
authors.initials: M
authors.lastname: Li
authors.affiliation: Medicine Research Center, Weifang Medical University, Weifang, Shandong, China.
authors.forename: Min
authors.initials: M
authors.lastname: Cheng
authors.affiliation: The Affiliated Hospital of Luzhou Medical College, Cardiothoracic Surgery, Luzhou, Sichuan Province, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Department of Anesthesiology, Traditional Chinese Medicine Hospital of Luzhou Medical College, Luzhou, Sichuan Province, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Anesthesiology, Traditional Chinese Medicine Hospital of Luzhou Medical College, Luzhou, Sichuan Province, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Chen
authors.affiliation: The Affiliated Hospital of Luzhou Medical College, Cardiothoracic Surgery, Luzhou, Sichuan Province, China.
authors.forename: Hongduan
authors.initials: H
authors.lastname: Liu
authors.affiliation: The Affiliated Hospital of Luzhou Medical College, Cardiothoracic Surgery, Luzhou, Sichuan Province, China.
authors.forename: Yibing
authors.initials: Y
authors.lastname: Fang
authors.affiliation: The Affiliated Hospital of Luzhou Medical College, Cardiothoracic Surgery, Luzhou, Sichuan Province, China.
authors.forename: Gen
authors.initials: G
authors.lastname: Zhang
authors.affiliation: The Affiliated Hospital of Luzhou Medical College, Cardiothoracic Surgery, Luzhou, Sichuan Province, China. yufengxu2015@sina.com.|Institute of Cardiovascular Research, Luzhou Medical College, Luzhou, Sichuan Province, China. yufengxu2015@sina.com.
authors.forename: Fengxu
authors.initials: F
authors.lastname: Yu
mesh_terms: D002478:Cells, Cultured; D015971:Gene Expression Regulation, Enzymologic; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D040542:Mechanotransduction, Cellular; D052250:Nitric Oxide Synthase Type III / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D051122:Scavenger Receptors, Class B / Q000235:genetics / Q000378:metabolism*; D013314:Stress, Mechanical; D013997:Time Factors; D014162:Transfection
chemical_list: C495124:SCARB1 protein, human; D051122:Scavenger Receptors, Class B; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III
keywords: SR-B1; eNOS; endothelial cell; mechanosensor; shear stress