title: Whole-genome resequencing of honeybee drones to detect genomic selection in a population managed for royal jelly.
abstract: Four main evolutionary lineages of A. mellifera have been described including eastern Europe (C) and western and northern Europe (M). Many apiculturists prefer bees from the C lineage due to their docility and high productivity. In France, the routine importation of bees from the C lineage has resulted in the widespread admixture of bees from the M lineage. The haplodiploid nature of the honeybee Apis mellifera, and its small genome size, permits affordable and extensive genomics studies. As a pilot study of a larger project to characterise French honeybee populations, we sequenced 60 drones sampled from two commercial populations managed for the production of honey and royal jelly. Results indicate a C lineage origin, whilst mitochondrial analysis suggests two drones originated from the O lineage. Analysis of heterozygous SNPs identified potential copy number variants near to genes encoding odorant binding proteins and several cytochrome P450 genes. Signatures of selection were detected using the hapFLK haplotype-based method, revealing several regions under putative selection for royal jelly production. The framework developed during this study will be applied to a broader sampling regime, allowing the genetic diversity of French honeybees to be characterised in detail.
authors.affiliation: GenPhySE, Université de Toulouse, INRA, INPT, INP-ENVT, 31326 Castanet Tolosan, France.
authors.forename: David
authors.initials: D
authors.lastname: Wragg
authors.affiliation: GenPhySE, Université de Toulouse, INRA, INPT, INP-ENVT, 31326 Castanet Tolosan, France.
authors.forename: Maria
authors.initials: M
authors.lastname: Marti-Marimon
authors.affiliation: Institut de l'abeille (ITSAP), UMT PrADE, 8914 Avignon, France.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Basso
authors.affiliation: GABI, INRA, AgroParisTech, Université Paris-Saclay, 78352 Jouy-en-Josas, France.
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Bidanel
authors.affiliation: GenPhySE, Université de Toulouse, INRA, INPT, INP-ENVT, 31326 Castanet Tolosan, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Labarthe
authors.affiliation: GenPhySE, Université de Toulouse, INRA, INPT, INP-ENVT, 31326 Castanet Tolosan, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Bouchez
authors.affiliation: INRA, UR 406 Abeilles et Environnement, UMT PrADE, 84914 Avignon, France.
authors.forename: Yves
authors.initials: Y
authors.lastname: Le Conte
authors.affiliation: GenPhySE, Université de Toulouse, INRA, INPT, INP-ENVT, 31326 Castanet Tolosan, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Vignal
mesh_terms: D000818:Animals; D001516:Bees / Q000235:genetics*; D005227:Fatty Acids; D005828:Genetics, Population; D059646:Genome Size; D049750:Genome, Insect; D006238:Haploidy; D010865:Pilot Projects; D020641:Polymorphism, Single Nucleotide; D012641:Selection, Genetic; D000073336:Whole Genome Sequencing / Q000379:methods*; D000099219:Royal Jelly
chemical_list: D005227:Fatty Acids; D000099219:Royal Jelly