title: Characterization of a Kunitz-type protease inhibitor (MjKuPI) reveals the involvement of MjKuPI positive hemocytes in the immune responses of kuruma shrimp Marsupenaeus japonicus.
abstract: Serine proteases and their inhibitors play vital roles in biological processes. Serine protease inhibitors, including Kunitz-type protease inhibitors play important roles not only in physiological process (i.e. blood clotting and fibrinolysis) but also in immune responses. In this study, we characterized a Kunitz-type protease inhibitor, designated MjKuPI, from kuruma shrimp Marsupenaeus japonicus. An expression profile showed that MjKuPI was mainly expressed in hemocytes. Immunostaining revealed that some hemocytes expressed MjKuPI (MjKuPI(+) hemocytes) and others did not (MjKuPI(-) hemocytes). Injection of shrimp with Vibrio penaeicida and white spot syndrome virus (WSSV) upregulated the mRNA level of MjKuPI, and a flow cytometry analysis revealed that the proportion of MjKuPI(+) hemocytes increased significantly 24 h after injection. Together, these results suggest that MjKuPI and MjKuPI(+) hemocytes have a role in the innate immune system of kuruma shrimp.
authors.affiliation: Laboratory of Genome Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo, 108-8477, Japan. Electronic address: mnhungts@gmail.com.
authors.forename: Hung Nam
authors.initials: HN
authors.lastname: Mai
authors.affiliation: College of Aquaculture and Fisheries, Cantho University, 3/2 Street, Cantho City, Viet Nam.
authors.forename: Ha Thi Nhu
authors.initials: HT
authors.lastname: Nguyen
authors.affiliation: Laboratory of Genome Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo, 108-8477, Japan.
authors.forename: Keiichiro
authors.initials: K
authors.lastname: Koiwai
authors.affiliation: Laboratory of Genome Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo, 108-8477, Japan.
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Kondo
authors.affiliation: Laboratory of Genome Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo, 108-8477, Japan.
authors.forename: Ikuo
authors.initials: I
authors.lastname: Hirono
mesh_terms: D000368:Aged; D000818:Animals; D007611:Aprotinin / Q000235:genetics / Q000378:metabolism*; D060829:Arthropod Proteins / Q000235:genetics / Q000378:metabolism*; D002469:Cell Separation; D004266:DNA Virus Infections / Q000276:immunology*; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D006434:Hemocytes / Q000276:immunology*; D006801:Humans; D007113:Immunity, Innate; D033561:Penaeidae / Q000276:immunology*; D015854:Up-Regulation; D014733:Vibrio / Q000276:immunology*; D014735:Vibrio Infections / Q000276:immunology*; D046848:White spot syndrome virus 1 / Q000276:immunology*
chemical_list: D060829:Arthropod Proteins; D007611:Aprotinin
keywords: Flow cytometry; Hemocyte marker; Marsupenaeus japonicus; MjKuPI; Vibrio penaeicida; WSSV