title: Expression Profiles of IGF-1R Gene and Polymorphisms of its Regulatory Regions in Different Pig Breeds.
abstract: Insulin like growth factor 1 receptor (IGF-1R) is a candidate gene for growth and carcass traits in regulating animal growth, metabolism and endocrine. It is widely expressed in liver, muscle, bone tissues where the IGF-1R functions as a factor that promotes cell growth. In this study, the protein expression level of IGF-1R gene in liver and muscle tissues of three periods (birth, weaning and adult) of three pig breeds (BamaXiang pigs (BM), Tibetan pigs (TM) and Junmu No.1 pigs (JM)) were tested by western blot. SNPs within the regulatory region of pig IGF-1R gene were detected using direct sequencing and then the genotypes were identified through AS-PCR approach. Results showed expression profiles of IGF-1R gene between liver and muscle tissues were different and significant differences were also found among pig breeds. In the same time, four SNPs were detected in the regulatory region of IGF-1R gene, among which the genotype frequency of three (g.-1468G > C, g.-1192 C > T and g.330,424 C > T) were significantly different among the pig breeds. BM tended to heterozygous (GC/CT) of the anterior two loci, while TM and JM preferred the other two homozygotes respectively. For the g.330,424 C > T, all pig breeds were tended to be the heterozygous. In conclusion, the SNPs with different genotype distribution among the three pig breeds may explain the gene expression difference between the different pig breeds.
authors.affiliation: College of Animal Science, Jilin University, 5333 Xi'an Road, Changchun, Jilin, 130062, China.
authors.forename: Yunyun
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: College of Animal Science, Jilin University, 5333 Xi'an Road, Changchun, Jilin, 130062, China.|Five-Star Animal Health Pharmaceutical Factory of Jilin Province, 5333 Xi'an Road, Changchun, Jilin, 130062, China.
authors.forename: Songcai
authors.initials: S
authors.lastname: Liu
authors.affiliation: China-Japan Union Hospital, Jilin University, 5333 Xi'an Road, Changchun, Jilin, 130062, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Animal Science, Jilin University, 5333 Xi'an Road, Changchun, Jilin, 130062, China.
authors.forename: Qingyan
authors.initials: Q
authors.lastname: Wu
authors.affiliation: College of Animal Science, Jilin University, 5333 Xi'an Road, Changchun, Jilin, 130062, China.
authors.forename: Siming
authors.initials: S
authors.lastname: Li
authors.affiliation: College of Animal Science, Jilin University, 5333 Xi'an Road, Changchun, Jilin, 130062, China.
authors.forename: Haoyu
authors.initials: H
authors.lastname: Fu
authors.affiliation: College of Animal Science, Jilin University, 5333 Xi'an Road, Changchun, Jilin, 130062, China.
authors.forename: Lijie
authors.initials: L
authors.lastname: Dong
authors.affiliation: College of Animal Science, Jilin University, 5333 Xi'an Road, Changchun, Jilin, 130062, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Yu
authors.affiliation: College of Animal Science, Jilin University, 5333 Xi'an Road, Changchun, Jilin, 130062, China. haolinlin@jlu.edu.cn.
authors.forename: Linlin
authors.initials: L
authors.lastname: Hao
mesh_terms: D000818:Animals; D001947:Breeding; D005260:Female; D005838:Genotype; D008297:Male; D020641:Polymorphism, Single Nucleotide; D017526:Receptor, IGF Type 1 / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D013552:Swine / Q000235:genetics*; D059467:Transcriptome
chemical_list: D017526:Receptor, IGF Type 1
keywords: IGF-1R gene; Pigs; Polymorphisms; Regulatory region; Tissue expression