title: Homologs of SCAR/WAVE complex components are required for epidermal cell morphogenesis in rice.
abstract: Filamentous actins (F-actins) play a vital role in epidermal cell morphogenesis. However, a limited number of studies have examined actin-dependent leaf epidermal cell morphogenesis events in rice. In this study, two recessive mutants were isolated: less pronounced lobe epidermal cell2-1 (lpl2-1) and lpl3-1, whose leaf and stem epidermis developed a smooth surface, with fewer serrated pavement cell (PC) lobes, and decreased papillae. The lpl2-1 also exhibited irregular stomata patterns, reduced plant height, and short panicles and roots. Molecular genetic studies demonstrated that LPL2 and LPL3 encode the PIROGI/Specifically Rac1-associated protein 1 (PIR/SRA1)-like and NCK-associated protein 1 (NAP1)-like proteins, respectively, two components of the suppressor of cAMP receptor/Wiskott-Aldrich syndrome protein-family verprolin-homologous protein (SCAR/WAVE) regulatory complex involved in actin nucleation and function. Epidermal cells exhibited abnormal arrangement of F-actins in both lpl2 and lpl3 expanding leaves. Moreover, the distorted trichomes of Arabidopsis pir could be partially restored by an overexpression of LPL2 A yeast two-hybrid assay revealed that LPL2 can directly interact with LPL3 in vitro Collectively, the results indicate that LPL2 and LPL3 are two functionally conserved homologs of the SCAR/WAVE complex components, and that they play an important role in controlling epidermal cell morphogenesis in rice by organising F-actin.
authors.affiliation: Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou 730000, China.
authors.forename: Wenqi
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou 730000, China.
authors.forename: Yuchuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou 730000, China.
authors.forename: Zhongliang
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou 730000, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Luo
authors.affiliation: Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou 730000, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.affiliation: Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou 730000, China.
authors.forename: Longfeng
authors.initials: L
authors.lastname: Yan
authors.affiliation: Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou 730000, China housw@lzu.edu.cn.
authors.forename: Suiwen
authors.initials: S
authors.lastname: Hou
mesh_terms: D000199:Actins / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D012275:Oryza / Q000378:metabolism / Q000502:physiology*; D019441:Plant Epidermis / Q000166:cytology / Q000254:growth & development*; D018515:Plant Leaves / Q000166:cytology; D010940:Plant Proteins / Q000502:physiology*; D018547:Plant Stems / Q000166:cytology; D054046:Plant Stomata / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D020798:Two-Hybrid System Techniques; D051300:Wiskott-Aldrich Syndrome Protein Family / Q000378:metabolism*
chemical_list: D000199:Actins; D010940:Plant Proteins; D051300:Wiskott-Aldrich Syndrome Protein Family
keywords: Epidermal cell; F-actin; SCAR/WAVE.; lobe; morphogenesis; rice