title: Molecular cloning and expression profiling of multiple Dof genes of Sorghum bicolor (L) Moench.
abstract: DNA binding with one finger (Dof) proteins represent a family of plant specific transcription factors associated with diverse biological processes, such as seed maturation and germination, phytohormone and light mediated regulation, and plant responses to biotic and abiotic stresses. In present study, a total of 21 Dof genes from Sorghum bicolor were cloned, sequenced and in silico characterized for homology search, revealing their identity to Dof like proteins. The expression profiling of SbDof genes using quantitative RT-PCR in different tissue types and also under drought and salt stresses was attempted. The SbDof genes displayed differential expression either in their transcript abundance or in their expression patterns under normal growth condition. Two of the SbDof genes namely SbDof8 and SbDof12 showed comparatively high level of transcript abundance in all the tissue types tested; whereas some of the SbDof genes showed a distinct tissue specific expression pattern. Further a total of 13 SbDof genes showed differential expression when subjected to either of the abiotic stress i.e. drought or salinity. Three of the SbDof genes namely SbDof12, SbDof19 and SbDof24 were found to be up-regulated in response to drought and salt stress. Comparative analysis of SbDof genes expression revealed existence of a complex transcriptional and functional diversity across plant growth and developmental stages.
authors.affiliation: Department of Biotechnology, D.D.U Gorakhpur University, Gorakhpur, Uttar Pradesh, 273 009, India.
authors.forename: Shubhra
authors.initials: S
authors.lastname: Gupta
authors.affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, 110 067, India.
authors.forename: Gulab C
authors.initials: GC
authors.lastname: Arya
authors.affiliation: Department of Biotechnology, D.D.U Gorakhpur University, Gorakhpur, Uttar Pradesh, 273 009, India.
authors.forename: Neha
authors.initials: N
authors.lastname: Malviya
authors.affiliation: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, 110 067, India.
authors.forename: Naveen C
authors.initials: NC
authors.lastname: Bisht
authors.affiliation: Department of Biotechnology, D.D.U Gorakhpur University, Gorakhpur, Uttar Pradesh, 273 009, India. dinesh_yad@rediffmail.com.
authors.forename: Dinesh
authors.initials: D
authors.lastname: Yadav
mesh_terms: D000222:Adaptation, Physiological; D003001:Cloning, Molecular; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D045868:Sorghum / Q000235:genetics* / Q000378:metabolism; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors
keywords: Abiotic stress; DNA binding with one finger (Dof); Expression profiling; Sorghum; Transcription factor