title: Genetic engineering of AtAOX1a in Saccharomyces cerevisiae prevents oxidative damage and maintains redox homeostasis.
abstract: This study aimed to validate the physiological importance of Arabidopsis thaliana alternative oxidase 1a (AtAOX1a) in alleviating oxidative stress using Saccharomyces cerevisiae as a model organism. The AOX1a transformant (pYES2AtAOX1a) showed cyanide resistant and salicylhydroxamic acid (SHAM)-sensitive respiration, indicating functional expression of AtAOX1a in S. cerevisiae. After exposure to oxidative stress, pYES2AtAOX1a showed better survival and a decrease in reactive oxygen species (ROS) when compared to S. cerevisiae with empty vector (pYES2). Furthermore, pYES2AtAOX1a sustained growth by regulating GPX2 and/or TSA2, and cellular NAD (+)/NADH ratio. Thus, the expression of AtAOX1a in S. cerevisiae enhances its respiratory tolerance which, in turn, maintains cellular redox homeostasis and protects from oxidative damage.
authors.affiliation: Department of Plant Sciences School of Life Sciences University of Hyderabad Hyderabad India.
authors.forename: Abhaypratap
authors.initials: A
authors.lastname: Vishwakarma
authors.affiliation: Department of Plant Sciences School of Life Sciences University of Hyderabad Hyderabad India.
authors.forename: Ahan
authors.initials: A
authors.lastname: Dalal
authors.affiliation: Department of Plant Sciences School of Life Sciences University of Hyderabad Hyderabad India.
authors.forename: Sarada Devi
authors.initials: SD
authors.lastname: Tetali
authors.affiliation: Department of Plant Sciences School of Life Sciences University of Hyderabad Hyderabad India.
authors.forename: Pulugurtha Bharadwaja
authors.initials: PB
authors.lastname: Kirti
authors.affiliation: Department of Biotechnology and Bioinformatics School of Life Sciences University of Hyderabad Hyderabad India.
authors.forename: Kollipara
authors.initials: K
authors.lastname: Padmasree
keywords: Saccharomyces cerevisiae; alternative oxidase 1a; oxidative stress; reactive oxygen species; redox homeostasis; respiration