title: Overcoming inter-subspecific hybrid sterility in rice by developing indica-compatible japonica lines.
abstract: Rice (Oryza sativa L.) is an important staple crop. The exploitation of the great heterosis that exists in the inter-subspecific crosses between the indica and japonica rice has long been considered as a promising way to increase the yield potential. However, the male and female sterility frequently occurred in the inter-subspecific hybrids hampered the utilization of the heterosis. Here we report that the inter-subspecific hybrid sterility in rice is mainly affected by the genes at Sb, Sc, Sd and Se loci for F1 male sterility and the gene at S5 locus for F1 female sterility. The indica-compatible japonica lines (ICJLs) developed by pyramiding the indica allele (S-i) at Sb, Sc, Sd and Se loci and the neutral allele (S-n) at S5 locus in japonica genetic background through marker-assisted selection are compatible with indica rice in pollen fertility and in spikelet fertility. These results showed a great promise of overcoming the inter-subspecific hybrid sterility and exploiting the heterosis by developing ICJLs.
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Guo
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Xu
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Wentao
authors.initials: W
authors.lastname: Li
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Wenyin
authors.initials: W
authors.lastname: Zhu
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Zhu
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Ziqiang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Luan
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Ziju
authors.initials: Z
authors.lastname: Dai
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Guifu
authors.initials: G
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Zemin
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Ruizhen
authors.initials: R
authors.lastname: Zeng
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Guang
authors.initials: G
authors.lastname: Tang
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Xuelin
authors.initials: X
authors.lastname: Fu
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Shaokui
authors.initials: S
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Guiquan
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000483:Alleles; D002678:Chimera / Q000235:genetics*; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D017343:Genes, Plant; D056426:Genetic Loci; D018745:Genome, Plant; D006823:Hybrid Vigor; D018895:Microsatellite Repeats; D012275:Oryza / Q000235:genetics*; D000069600:Plant Breeding / Q000379:methods*; D051479:Plant Infertility / Q000235:genetics; D011058:Pollen / Q000235:genetics; D012641:Selection, Genetic