title: Efficient non-enzymatic cleavage of Staphylococcus aureus plasmid DNAs mediated by neodymium ions.
abstract: Staphylococcus aureus plasmids are the main factor in the spreading of antibacterial resistance among bacterial strains that has emerged on a worldwide scale. Plasmids recovered from 12 clinical and food isolates of S. aureus were treated with 10 mM free lanthanide Nd(3+) ions (non-enzymatic cleavage agent) in Hepes buffer (pH 7.5) at 70 °C. Topological forms of plasmids-closed circular (ccc), open circular (oc), and linear (lin)-produced by cleavage at different times were separated using pulsed-field agarose gel electrophoresis. The method is proposed to detect and differentiate several plasmids in the same bacterial strain according to their size.
authors.affiliation: Faculty of Chemistry, Brno University of Technology, CZ-612 00 Brno, Czech Republic.
authors.forename: Monika
authors.initials: M
authors.lastname: Zovčáková
authors.affiliation: Faculty of Chemistry, Brno University of Technology, CZ-612 00 Brno, Czech Republic.
authors.forename: Alena
authors.initials: A
authors.lastname: Španová
authors.affiliation: Department of Experimental Biology, Faculty of Science, Masaryk University, CZ-611 37 Brno, Czech Republic.
authors.forename: Roman
authors.initials: R
authors.lastname: Pantůček
authors.affiliation: Department of Experimental Biology, Faculty of Science, Masaryk University, CZ-611 37 Brno, Czech Republic.
authors.forename: Jiří
authors.initials: J
authors.lastname: Doškař
authors.affiliation: Faculty of Chemistry, Brno University of Technology, CZ-612 00 Brno, Czech Republic. Electronic address: rittich@fch.vut.cz.
authors.forename: Bohuslav
authors.initials: B
authors.lastname: Rittich
mesh_terms: D004269:DNA, Bacterial / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D007477:Ions / Q000737:chemistry / Q000494:pharmacology; D009354:Neodymium / Q000737:chemistry / Q000494:pharmacology*; D010957:Plasmids / Q000235:genetics / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D007477:Ions; D009354:Neodymium
keywords: Lanthanide Nd(3+) ions; Plasmid DNA; Plasmid size; Pulsed-field agarose gel electrophoresis; Staphylococcus aureus