title: Escherichia coli-based cell-free extract development for protein-based cancer therapeutic production.
abstract: Cell-free protein synthesis has been around for decades but it has never been close to becoming a robust tool for the production of biotherapeutic agents. In this review, we focus on how Escherichia coli-based cell-free protein synthesis can be modified in various ways to produce challenging, complex anticancer biotherapeutics. Here we report progress in extract preparation and its relation to cell-free cancer research. The future prospects of cell-free technology and its potential in various areas of cancer therapeutics production are also highlighted.
authors.affiliation: Department of Chemical Engineering, Brigham Young University, Provo, UT, USA.
authors.forename: Amin S M
authors.initials: AS
authors.lastname: Salehi
authors.forename: Conner C
authors.initials: CC
authors.lastname: Earl
authors.forename: Christina
authors.initials: C
authors.lastname: Muhlestein
authors.forename: Bradley C
authors.initials: BC
authors.lastname: Bundy
mesh_terms: D000970:Antineoplastic Agents / Q000627:therapeutic use; D002474:Cell-Free System; D004926:Escherichia coli / Q000378:metabolism*; D009369:Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D014176:Protein Biosynthesis; D012106:Research
chemical_list: D000970:Antineoplastic Agents