title: Immunohistochemical Detection of Chikungunya Virus Antigens in Formalin-Fixed and Paraffin-Embedded Tissues.
abstract: Immunohistochemistry is a histological technique that allows detection of one or more proteins of interest within a cell using specific antibody binding, followed by microscopic visualization of a chromogenic substrate catalyzed by peroxidase and/or alkaline phosphatase. Here, we describe a method to localize Chikungunya virus (CHIKV) antigens in formalin-fixed and paraffin-embedded infected mouse brain.
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
authors.forename: Chun Wei
authors.initials: CW
authors.lastname: Chiam
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
authors.forename: I-Ching
authors.initials: IC
authors.lastname: Sam
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
authors.forename: Yoke Fun
authors.initials: YF
authors.lastname: Chan
authors.affiliation: Department of Pathology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
authors.forename: Kum Thong
authors.initials: KT
authors.lastname: Wong
authors.affiliation: Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia. kcong@um.edu.my.
authors.forename: Kien Chai
authors.initials: KC
authors.lastname: Ong
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000914:Antibodies, Viral / Q000378:metabolism*; D000956:Antigens, Viral / Q000276:immunology*; D002646:Chikungunya virus / Q000276:immunology / Q000378:metabolism*; D005557:Formaldehyde; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D018613:Microscopy, Confocal; D016612:Paraffin Embedding; D016707:Tissue Fixation
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D005557:Formaldehyde
keywords: Antibody; Chikungunya virus; Formalin-fixed; Immunohistochemistry; Paraffin-embedded