title: Hepatocyte growth factor-induced differentiation of bone mesenchymal stem cells toward hepatocyte-like cells occurs through nuclear factor-kappa B signaling in vitro.
abstract: Hepatocyte growth factor (HGF) is multifaceted cytokine that regulates proliferation, differentiation, morphology, and motility within numerous stem cells. More recently, HGF has been reported to induce the differentiation of bone mesenchymal stem cells (BMSCs) into mature hepatocytes, but the underlying biochemical and molecular signaling is largely unknown. We isolated BMSC from the bone marrow of rats, which were then cultured and exposed to HGF for 15 days. We subsequently assayed these cells for liver functionality and markers, and blocked NF-кB signaling at various stages of the pathway. The present results demonstrate that HGF induces the differentiation of BMSCs toward hepatocyte-like cells through the NF-кB signaling. More specifically, HGF upregulated the translocation of NF-кB to the nucleus.
authors.affiliation: Department of Cell Biology, School of Basic Medicine, Qingdao University, Qingdao City, Shandong Province, 266000, China.
authors.forename: Tongxi
authors.initials: T
authors.lastname: Yang
authors.affiliation: Department of Cell Biology, School of Basic Medicine, Qingdao University, Qingdao City, Shandong Province, 266000, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Cell Biology, School of Basic Medicine, Qingdao University, Qingdao City, Shandong Province, 266000, China.
authors.forename: Shasha
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Department of Histology and Embryology, School of Basic Medicine, Qingdao University, Qingdao City, Shandong Province, 266000, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Cell Biology, School of Basic Medicine, Qingdao University, Qingdao City, Shandong Province, 266000, China.
authors.forename: Zhongjie
authors.initials: Z
authors.lastname: Yu
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D017228:Hepatocyte Growth Factor / Q000494:pharmacology*; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D016328:NF-kappa B; D017228:Hepatocyte Growth Factor
keywords: BMSCs; NF-кB; differentiation; hepatocyte; indocyanine green