title: Viral-Host Protein Interaction Studies Using Yeast Two-Hybrid Screening Method.
abstract: Yeast two-hybrid (Y2H) assay is one of the earliest methods developed to study protein-protein interactions. In the proteomics era, Y2H has created a niche of its own by providing protein interaction maps for various organisms. Owing to limited coding capacities of their genomes, viruses are dependent on their host cellular machinery for successful infection. Identification of the key players orchestrating the survival of virus in their host is essential for understanding viral life cycle and devising strategies to prevent interactions resulting in pathogenesis. In this chapter, Y2H assay will be explained in detail for studying viral-host protein interactions of Chikungunya virus (CHIKV).
authors.affiliation: Centre for Emerging Diseases, Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector 62, Noida, 201307, Uttar Pradesh, India.
authors.forename: Namrata
authors.initials: N
authors.lastname: Dudha
authors.affiliation: Centre for Emerging Diseases, Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector 62, Noida, 201307, Uttar Pradesh, India. sanjay.gupta@jiit.ac.in.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Gupta
mesh_terms: D002646:Chikungunya virus / Q000502:physiology*; D054884:Host-Pathogen Interactions; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D040901:Proteomics; D012441:Saccharomyces cerevisiae / Q000378:metabolism / Q000821:virology*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D020798:Two-Hybrid System Techniques; D014764:Viral Proteins / Q000378:metabolism
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D014764:Viral Proteins
keywords: Chikungunya virus; Human cDNA library; Protein–protein interactions; Virus–host interactions; Yeast two-hybrid