title: Integrin-Linked Kinase Senses Hypoxia During Scar Angiogenesis.
abstract: Integrin-linked kinase (ILK) mediates signal transduction between cells and the extracellular matrix, regulating cell proliferation, migration, angiogenesis, and apoptosis. However, its roles in the formation of hypertrophic scars are not yet clear. In this study, we found that ILK was predominantly expressed on the microvascular endothelial cells and the epidermal basal cells of human hypertrophic scars. The proliferation, migration and angiogenesis of primary human scar microvascular endothelial cells (HSMECs) were significantly inhibited after ILK was silenced. The ILK inhibitor QLT0267 had the same effect of impeding angiogenesis in vitro by blocking ILK activity. Both siRNA and QLT0267 markedly decreased the expression of vascular endothelial growth factor, but not its receptors, such as human vascular endothelial cell growth factor receptor 1 or kinase insert domain-containing receptor. We also showed that the expression of ILK was enhanced by inducing mild hypoxia with CoCl2, but it was suppressed under serious hypoxia. Thus, ILK regulates HSMEC proliferation and angiogenesis and participates in the formation of hypertrophic scars, in which mild hypoxia may be the mechanism of action.
authors.affiliation: 1 Guangzhou Red Cross Hospital, Jinan University, Guangzhou, China.
authors.forename: Yeyang
authors.initials: Y
authors.lastname: Li
authors.affiliation: 1 Guangzhou Red Cross Hospital, Jinan University, Guangzhou, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.affiliation: 1 Guangzhou Red Cross Hospital, Jinan University, Guangzhou, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Hu
authors.affiliation: 1 Guangzhou Red Cross Hospital, Jinan University, Guangzhou, China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Lin
authors.affiliation: 1 Guangzhou Red Cross Hospital, Jinan University, Guangzhou, China.
authors.forename: Jingen
authors.initials: J
authors.lastname: Sun
authors.affiliation: 1 Guangzhou Red Cross Hospital, Jinan University, Guangzhou, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Mi
authors.affiliation: 1 Guangzhou Red Cross Hospital, Jinan University, Guangzhou, China.
authors.forename: Renkun
authors.initials: R
authors.lastname: Wang
authors.affiliation: 1 Guangzhou Red Cross Hospital, Jinan University, Guangzhou, China.
authors.forename: Jinlun
authors.initials: J
authors.lastname: Wang
authors.affiliation: 1 Guangzhou Red Cross Hospital, Jinan University, Guangzhou, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Wang
authors.affiliation: 1 Guangzhou Red Cross Hospital, Jinan University, Guangzhou, China.
authors.forename: Rixing
authors.initials: R
authors.lastname: Zhou
mesh_terms: D002921:Cicatrix; D006801:Humans; D000860:Hypoxia; D009389:Neovascularization, Pathologic; D017346:Protein Serine-Threonine Kinases / Q000502:physiology*; D042461:Vascular Endothelial Growth Factor A; D000099200:Scaffold Protein ILK
chemical_list: D017346:Protein Serine-Threonine Kinases; D042461:Vascular Endothelial Growth Factor A; D000099200:Scaffold Protein ILK
keywords: angiogenesis; human scar microvascular endothelial cells; hypertrophic scar; integrin-linked kinase