title: Epigenetic regulation of reelin expression in multiple myeloma.
abstract: Reelin (RELN) is a secreted extracellular matrix glycoprotein associated with the positioning and migration of neuronal cells and a few types of non-neuronal cells. We have previously reported RELN expression in multiple myeloma cells. High RELN expression was associated with poor prognosis and enhanced myeloma cell adhesion and survival. To examine the epigenetic regulation of RELN expression, its promoter methylation status in myeloma-derived cell lines and primary tumour cells from myeloma patients were analysed. RELN expression was moderate in CD19+B cells and was upregulated in CD138+plasma cells. A further upregulated RELN transcription was found in multiple myeloma cells. High expressions of RELN in myeloma cell lines as well as in patients were associated with hypomethylation in RELN promoter region. Demethylation increased RELN transcription in vitro. Together, we established that the methylation status of the promoter proximal cytosine-phospho-guanine dinucleotides determines the expression of RELN in myeloma cells. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Key Laboratory of Medical Immunology, Ministry of Health, Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Lin
authors.affiliation: Key Laboratory of Medical Immunology, Ministry of Health, Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.
authors.forename: Pingzhang
authors.initials: P
authors.lastname: Wang
authors.affiliation: Key Laboratory of Medical Immunology, Ministry of Health, Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Liu
authors.affiliation: Jining No.1 People's Hospital, Jining, Shandong, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Medical Immunology, Ministry of Health, Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Medical Immunology, Ministry of Health, Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Hao
authors.affiliation: Hangzhou Cancer Hospital, Hang Zhou, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Liang
authors.affiliation: Peking University Institute of Hematology, People's Hospital, Beijing, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Huang
authors.affiliation: Peking University Institute of Hematology, People's Hospital, Beijing, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Lu
authors.affiliation: Key Laboratory of Medical Immunology, Ministry of Health, Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Ge
mesh_terms: D000107:Acetylation; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics*; D045744:Cell Line, Tumor; D044127:Epigenesis, Genetic; D016326:Extracellular Matrix Proteins / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006657:Histones / Q000378:metabolism; D006801:Humans; D035683:MicroRNAs / Q000235:genetics; D009101:Multiple Myeloma / Q000235:genetics* / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D000091042:Reelin Protein; D012697:Serine Endopeptidases / Q000235:genetics*; D015854:Up-Regulation
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; D006657:Histones; D035683:MicroRNAs; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D000091042:Reelin Protein; C000717127:RELN protein, human; D012697:Serine Endopeptidases
keywords: methylation; microRNA; multiple myeloma; reelin