title: Molecularly imprinted polymers as the extracted sorbents of clenbuterol ahead of liquid chromatographic determination.
abstract: A pre-treatment methodology for clenbuterol hydrochloride (CLEN) isolation and enrichment in a complex matrix environment was developed through exploiting molecularly imprinted polymers (MIPs). CLEN-imprinted polymers were synthesized by the combined use of ally-β-cyclodextrin (ally-β-CD) and methacrylic acid (MAA), allyl-β-CD and acrylonitrile (AN), and allyl-β-CD and methyl methacrylate (MMA) as the binary functional monomers. MAA-linked allyl-β-CD MIPs (M-MAA) were characterized by Fourier transform-infrared (FT-IR) spectroscopy and a scanning electron microscope (SEM). Based upon the results, M-MAA polymers generally proved to be an excellent selective extraction compared to its references: AN-linked allyl-β-CD MIPs (M-AN) and MMA-linked allyl-β-CD MIPs (M-MMA). M-MAA polymers were eventually chosen to run through a molecularly imprinted solid-phase extraction (MISPE) micro-column to enrich CLEN residues spiked in pig livers. A high recovery was achieved, ranging from 91.03% to 96.76% with relative standard deviation (RSD) ≤4.45%.
authors.affiliation: Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, China.
authors.forename: Sovichea
authors.initials: S
authors.lastname: Lay
authors.affiliation: College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310032, China.
authors.forename: Hai-Ning
authors.initials: HN
authors.lastname: Yu
authors.affiliation: Department of Chemical Engineering and Material Science, Zhejiang University of Technology, Hangzhou 310032, China.
authors.forename: Bao-Xiang
authors.initials: BX
authors.lastname: Hu
authors.affiliation: Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, China.
authors.forename: Sheng-Rong
authors.initials: SR
authors.lastname: Shen
mesh_terms: D000327:Adsorption; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D002976:Clenbuterol / Q000032:analysis* / Q000737:chemistry; D008099:Liver / Q000737:chemistry*; D008689:Methacrylates / Q000737:chemistry; D054802:Molecular Imprinting; D013552:Swine; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: D008689:Methacrylates; D047392:beta-Cyclodextrins; C008384:methacrylic acid; D002976:Clenbuterol
keywords: Clenbuterol; Cyclodextrin; Molecularly imprinted polymer (MIP); Molecularly imprinted solid-phase extraction (MISPE)