title: Hyperthermostable Thermotoga maritima xylanase XYN10B shows high activity at high temperatures in the presence of biomass-dissolving hydrophilic ionic liquids.
abstract: The gene of Thermotoga maritima GH10 xylanase (TmXYN10B) was synthesised to study the extreme limits of this hyperthermostable enzyme at high temperatures in the presence of biomass-dissolving hydrophilic ionic liquids (ILs). TmXYN10B expressed from Pichia pastoris showed maximal activity at 100 °C and retained 92 % of maximal activity at 105 °C in a 30-min assay. Although the temperature optimum of activity was lowered by 1-ethyl-3-methylimidazolium acetate ([EMIM]OAc), TmXYN10B retained partial activity in 15-35 % hydrophilic ILs, even at 75-90 °C. TmXYN10B retained over 80 % of its activity at 90 °C in 15 % [EMIM]OAc and 15-25 % 1-ethyl-3-methylimidazolium dimethylphosphate ([EMIM]DMP) during 22-h reactions. [EMIM]OAc may rigidify the enzyme and lower V max. However, only minor changes in kinetic parameter K m showed that competitive inhibition by [EMIM]OAc of TmXYN10B is minimal. In conclusion, when extended enzymatic reactions under extreme conditions are required, TmXYN10B shows extraordinary potential.
authors.affiliation: South-Central University for Nationalities, College of Life Science, Wuhan, 430074, China.
authors.forename: Tianyi
authors.initials: T
authors.lastname: Yu
authors.affiliation: Department of Biotechnology and Chemical Technology, School of Chemical Technology, Aalto University, 00076, Aalto, Finland.
authors.forename: Sasikala
authors.initials: S
authors.lastname: Anbarasan
authors.affiliation: South-Central University for Nationalities, College of Life Science, Wuhan, 430074, China.
authors.forename: Yawei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Biotechnology and Chemical Technology, School of Chemical Technology, Aalto University, 00076, Aalto, Finland.
authors.forename: Kübra
authors.initials: K
authors.lastname: Telli
authors.affiliation: Department of Biotechnology and Chemical Technology, School of Chemical Technology, Aalto University, 00076, Aalto, Finland.
authors.forename: Aşkın Sevinç
authors.initials: AS
authors.lastname: Aslan
authors.affiliation: Hubei University of Technology, Wuhan, 430068, China.
authors.forename: Zhengding
authors.initials: Z
authors.lastname: Su
authors.affiliation: Wuhan Sunhy Biology Co., Ltd, Wuhan, 430074, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: South-Central University for Nationalities, College of Life Science, Wuhan, 430074, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Biotechnology and Chemical Technology, School of Chemical Technology, Aalto University, 00076, Aalto, Finland.
authors.forename: Piia
authors.initials: P
authors.lastname: Iivonen
authors.affiliation: Department of Biotechnology and Chemical Technology, School of Chemical Technology, Aalto University, 00076, Aalto, Finland.
authors.forename: Sami
authors.initials: S
authors.lastname: Havukainen
authors.affiliation: Department of Biotechnology and Chemical Technology, School of Chemical Technology, Aalto University, 00076, Aalto, Finland.
authors.forename: Tero
authors.initials: T
authors.lastname: Mentunen
authors.affiliation: Department of Forest Products Technology, School of Chemical Technology, Aalto University, 00076, Aalto, Finland.
authors.forename: Michael
authors.initials: M
authors.lastname: Hummel
authors.affiliation: Department of Forest Products Technology, School of Chemical Technology, Aalto University, 00076, Aalto, Finland.
authors.forename: Herbert
authors.initials: H
authors.lastname: Sixta
authors.affiliation: Department of Bioengineering, Gebze Technical University, 41400, Gebze Kocaeli, Turkey.
authors.forename: Baris
authors.initials: B
authors.lastname: Binay
authors.affiliation: Department of Biotechnology and Chemical Technology, School of Chemical Technology, Aalto University, 00076, Aalto, Finland. ossi.turunen@aalto.fi.
authors.forename: Ossi
authors.initials: O
authors.lastname: Turunen
authors.affiliation: South-Central University for Nationalities, College of Life Science, Wuhan, 430074, China. xionghr@mail.scuec.edu.cn.
authors.forename: Hairong
authors.initials: H
authors.lastname: Xiong
mesh_terms: D001426:Bacterial Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D018533:Biomass; D043364:Endo-1,4-beta Xylanases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004795:Enzyme Stability; D006358:Hot Temperature; D007218:Industrial Microbiology; D052578:Ionic Liquids / Q000494:pharmacology*; D010843:Pichia / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D020124:Thermotoga maritima / Q000201:enzymology* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004791:Enzyme Inhibitors; D052578:Ionic Liquids; D043364:Endo-1,4-beta Xylanases
keywords: Competitive inhibition; Expression in Pichia pastoris; Extreme stability; GH10 xylanase; Ionic liquids; Thermotoga maritima