title: Cloning and Characterization of Sirtuin3 (SIRT3).
abstract: Mitochondria play a pivotal role in maintaining cellular homeostasis and regulating longevity. SIRT3 is a mitochondrial sirtuin mediating the deacetylation of various metabolic and antioxidant enzymes, in turn controlling energy metabolism, stress resistance, and the pace of ageing. To study the function of SIRT3, a proteomics-based approach is employed for identifying the protein-binding partners of this enzyme in mitochondria.
authors.affiliation: Department of Pharmacology and Pharmacy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Level 2, Laboratory Block, 21 Sassoon Road, Pokfulam, Hong Kong, China.
authors.forename: Andy W C
authors.initials: AW
authors.lastname: Man
authors.affiliation: Department of Pharmacology and Pharmacy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Level 2, Laboratory Block, 21 Sassoon Road, Pokfulam, Hong Kong, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Bai
authors.affiliation: Department of Pharmacology and Pharmacy, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Level 2, Laboratory Block, 21 Sassoon Road, Pokfulam, Hong Kong, China. yuwanghk@hku.hk.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000107:Acetylation; D002453:Cell Cycle; D003001:Cloning, Molecular / Q000379:methods*; D004734:Energy Metabolism; D057809:HEK293 Cells; D056945:Hep G2 Cells; D006706:Homeostasis; D006801:Humans; D008928:Mitochondria / Q000378:metabolism; D060066:Protein Interaction Maps; D040901:Proteomics / Q000379:methods*; D056566:Sirtuin 3 / Q000235:genetics* / Q000378:metabolism*
chemical_list: C466093:SIRT3 protein, human; D056566:Sirtuin 3
keywords: Acetylation; Mitochondria; Protein–protein interaction; Sirtuin