title: V-ATPase activity in the TGN/EE is required for exocytosis and recycling in Arabidopsis.
abstract: In plants, vacuolar H(+)-ATPase (V-ATPase) activity acidifies both the trans-Golgi network/early endosome (TGN/EE) and the vacuole. This dual V-ATPase function has impeded our understanding of how the pH homeostasis within the plant TGN/EE controls exo- and endocytosis. Here, we show that the weak V-ATPase mutant deetiolated3 (det3) displayed a pH increase in the TGN/EE, but not in the vacuole, strongly impairing secretion and recycling of the brassinosteroid receptor and the cellulose synthase complexes to the plasma membrane, in contrast to mutants lacking tonoplast-localized V-ATPase activity only. The brassinosteroid insensitivity and the cellulose deficiency defects in det3 were tightly correlated with reduced Golgi and TGN/EE motility. Thus, our results provide strong evidence that acidification of the TGN/EE, but not of the vacuole, is indispensable for functional secretion and recycling in plants.
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Gent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors.forename: Yu
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Developmental Biology of Plants, Centre for Organismal Studies (COS), Heidelberg University, 69120 Heidelberg, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Scholl
authors.affiliation: Max-Planck Institute for Molecular Plant Physiology, 14476 Potsdam, Germany.
authors.forename: Anett
authors.initials: A
authors.lastname: Doering
authors.affiliation: Max-Planck Institute for Molecular Plant Physiology, 14476 Potsdam, Germany.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Gent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors.forename: Niloufer G
authors.initials: NG
authors.lastname: Irani
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Gent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors.forename: Simone Di
authors.initials: SD
authors.lastname: Rubbo
authors.affiliation: Max-Planck Institute for Molecular Plant Physiology, 14476 Potsdam, Germany.
authors.forename: Lutz
authors.initials: L
authors.lastname: Neumetzler
authors.affiliation: Max-Planck Institute for Molecular Plant Physiology, 14476 Potsdam, Germany.
authors.forename: Praveen
authors.initials: P
authors.lastname: Krishnamoorthy
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Gent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Van Houtte
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Gent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors.forename: Evelien
authors.initials: E
authors.lastname: Mylle
authors.affiliation: Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1318, Institut Jean-Pierre Bourgin, Saclay Plant Sciences, 78000 Versailles, France.|AgroParisTech,Institut Jean-Pierre Bourgin, 78000 Versailles, France.
authors.forename: Volker
authors.initials: V
authors.lastname: Bischoff
authors.affiliation: Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1318, Institut Jean-Pierre Bourgin, Saclay Plant Sciences, 78000 Versailles, France.|AgroParisTech,Institut Jean-Pierre Bourgin, 78000 Versailles, France.
authors.forename: Samantha
authors.initials: S
authors.lastname: Vernhettes
authors.affiliation: Department of Organic Chemistry, Polymer Chemistry Research Group and Laboratory for Organic Synthesis, Ghent University, 9000 Gent, Belgium.
authors.forename: Johan
authors.initials: J
authors.lastname: Winne
authors.affiliation: Institute of Science and Technology Austria (IST Austria), 3400 Klosterneuburg, Austria.
authors.forename: Jiří
authors.initials: J
authors.lastname: Friml
authors.affiliation: Center for Plant Molecular Biology (ZMBP), University of Tübingen, 72076 Tübingen, Germany.
authors.forename: York-Dieter
authors.initials: YD
authors.lastname: Stierhof
authors.affiliation: Developmental Biology of Plants, Centre for Organismal Studies (COS), Heidelberg University, 69120 Heidelberg, Germany.
authors.forename: Karin
authors.initials: K
authors.lastname: Schumacher
authors.affiliation: Max-Planck Institute for Molecular Plant Physiology, 14476 Potsdam, Germany.|Australian Research Council, Centre of Excellence in Plant Cell Walls, School of Botany, University of Melbourne, Parkville, Victoria 3010, Australia.
authors.forename: Staffan
authors.initials: S
authors.lastname: Persson
authors.affiliation: Department of Plant Systems Biology, VIB, 9052 Gent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
authors.forename: Eugenia
authors.initials: E
authors.lastname: Russinova