title: Analysis of the In Vivo Turnover of CD4+ T-Cell Subsets in Chronically SIV-Infected Sooty Mangabeys.
abstract: Aberrant turnover of memory CD4+ T-cells is central to Acquired Immunodeficiency Syndrome (AIDS) progression. Understanding the relationship between the turnover of CD4+ subsets and immunological homeostasis during simian immunodeficiency virus (SIV) infection in natural hosts may provide insight into mechanisms of immune regulation that may serve as models for therapeutic intervention in Human Immunodeficiency Virus (HIV)-infected persons. Sooty mangabeys (SMs) have naturally evolved with SIV to avoid AIDS progression while maintaining healthy peripheral CD4+ T-cell counts and thus represent a model by which therapeutic interventions for AIDS progression might be elucidated. To assess the relationship between the turnover of CD4+ subsets and immunological homeostasis during SIV infection in non-progressive hosts, we treated 6 SIV-uninfected and 9 SIV-infected SMs with 2'-bromo-5'-deoxyuridine (BrdU) for 14 days and longitudinally assessed CD4+ T-cell subset turnover by polychromatic flow cytometry. We observed that, in SIV-infected SMs, turnover of CD4+ T-cell naïve and central, transitional, and effector memory subsets is comparable to that in uninfected animals. Comparable turnover of CD4+ T-cell subsets irrespective of SIV-infection status likely contributes to the lack of aberrant immune activation and disease progression observed after infection in non-progressive hosts.
authors.affiliation: Yerkes National Primate Research Center, and Emory Vaccine Center, Emory University, Atlanta, 30322, Georgia, United States of America.|Laboratory of Molecular Microbiology, National Institutes of Health, Bethesda, 20892, Maryland, United States of America.
authors.forename: Alexandra M
authors.initials: AM
authors.lastname: Ortiz
authors.affiliation: Yerkes National Primate Research Center, and Emory Vaccine Center, Emory University, Atlanta, 30322, Georgia, United States of America.
authors.forename: Diane G
authors.initials: DG
authors.lastname: Carnathan
authors.affiliation: Yerkes National Primate Research Center, and Emory Vaccine Center, Emory University, Atlanta, 30322, Georgia, United States of America.
authors.forename: Joana
authors.initials: J
authors.lastname: Yu
authors.affiliation: Yerkes National Primate Research Center, and Emory Vaccine Center, Emory University, Atlanta, 30322, Georgia, United States of America.
authors.forename: Katherine M
authors.initials: KM
authors.lastname: Sheehan
authors.affiliation: Complex Systems in Biology Group, Centre for Vascular Research, University of New South Wales, Sydney, 2052, Australia.
authors.forename: Peter
authors.initials: P
authors.lastname: Kim
authors.affiliation: Complex Systems in Biology Group, Centre for Vascular Research, University of New South Wales, Sydney, 2052, Australia.
authors.forename: Arnold
authors.initials: A
authors.lastname: Reynaldi
authors.affiliation: Yerkes National Primate Research Center, and Emory Vaccine Center, Emory University, Atlanta, 30322, Georgia, United States of America.
authors.forename: Thomas H
authors.initials: TH
authors.lastname: Vanderford
authors.affiliation: Laboratory of Molecular Microbiology, National Institutes of Health, Bethesda, 20892, Maryland, United States of America.|Department of Pharmaceutics, University of Washington, Seattle, 98195, Washington, United States of America.
authors.forename: Nichole R
authors.initials: NR
authors.lastname: Klatt
authors.affiliation: Laboratory of Molecular Microbiology, National Institutes of Health, Bethesda, 20892, Maryland, United States of America.
authors.forename: Jason M
authors.initials: JM
authors.lastname: Brenchley
authors.affiliation: Complex Systems in Biology Group, Centre for Vascular Research, University of New South Wales, Sydney, 2052, Australia.
authors.forename: Miles P
authors.initials: MP
authors.lastname: Davenport
authors.affiliation: Yerkes National Primate Research Center, and Emory Vaccine Center, Emory University, Atlanta, 30322, Georgia, United States of America.
authors.forename: Guido
authors.initials: G
authors.lastname: Silvestri
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology* / Q000276:immunology / Q000821:virology; D016665:Cercocebus atys; D018450:Disease Progression; D008213:Lymphocyte Activation; D016097:Simian Acquired Immunodeficiency Syndrome / Q000473:pathology / Q000821:virology*; D015302:Simian Immunodeficiency Virus / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000166:cytology* / Q000276:immunology / Q000821:virology; D019562:Viral Load