title: Subcellular chemical and morphological analysis by stimulated Raman scattering microscopy and image analysis techniques.
abstract: The visualization of heterogeneous morphology, segmentation and quantification of image features is a crucial point for nonlinear optics microscopy applications, spanning from imaging of living cells or tissues to biomedical diagnostic. In this paper, a methodology combining stimulated Raman scattering microscopy and image analysis technique is presented. The basic idea is to join the potential of vibrational contrast of stimulated Raman scattering and the strength of imaging analysis technique in order to delineate subcellular morphology with chemical specificity. Validation tests on label free imaging of polystyrene-beads and of adipocyte cells are reported and discussed.
authors.affiliation: National Research Council (CNR), Institute for Microelectronics and Microsystems, I-80131 Napoli, Italy; Second University of Naples (SUN), Department of Information Engineering, I-81031 Aversa, Italy.
authors.forename: Annalisa
authors.initials: A
authors.lastname: D'Arco
authors.affiliation: National Research Council (CNR), Institute for High-Performance Computing and Networking, I-80131 Napoli, Italy.
authors.forename: Nadia
authors.initials: N
authors.lastname: Brancati
authors.affiliation: National Research Council (CNR), Institute for Microelectronics and Microsystems, I-80131 Napoli, Italy.
authors.forename: Maria Antonietta
authors.initials: MA
authors.lastname: Ferrara
authors.affiliation: National Research Council (CNR), Institute for Microelectronics and Microsystems, I-80131 Napoli, Italy.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Indolfi
authors.affiliation: National Research Council (CNR), Institute for High-Performance Computing and Networking, I-80131 Napoli, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Frucci
authors.affiliation: National Research Council (CNR), Institute for Microelectronics and Microsystems, I-80131 Napoli, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Sirleto
keywords: (100.0100) Image processing; (170.0110) Imaging systems; (180.4315) Nonlinear microscopy; (190.5890) Scattering, stimulated; (190.7110) Ultrafast nonlinear optics