title: Efficient role of IgH 3' regulatory region deficient B-cells in the development of oil granulomas.
abstract: Functional B-cells are essential for the formation of oil granulomas. The IgH 3' regulatory region (3'RR) activates important check-points during B-cell maturation. We investigated if 3'RR-deficient B-cells remain efficient to develop oil granulomas in response to pristine. B-cells expressing an IgH 3'RR-deficient allele were similarly recruited to wild type allele expressing B-cells in the granuloma. No differences were observed between 3'RR-deficient mice and control mice for granuloma numbers, cellular composition and ability to express mRNA transcripts for several pro- and anti-inflammatory cytokines. Altogether these results suggest a normal role for 3'RR-deficient B-cells in the development of an acute B-cell-mediated inflammatory response.
authors.affiliation: Department of Immunology, CNRS UMR 7276, CRIBL, Université de Limoges, Limoges, France.
authors.forename: Nour
authors.initials: N
authors.lastname: Ghazzaui
authors.affiliation: Department of Immunology, CNRS UMR 7276, CRIBL, Université de Limoges, Limoges, France.
authors.forename: Alexis
authors.initials: A
authors.lastname: Saintamand
authors.affiliation: Department of Immunology, CNRS UMR 7276, CRIBL, Université de Limoges, Limoges, France.
authors.forename: Hussein
authors.initials: H
authors.lastname: Issaoui
authors.affiliation: Department of Immunology, CNRS UMR 7276, CRIBL, Université de Limoges, Limoges, France.
authors.forename: Faten
authors.initials: F
authors.lastname: Saad
authors.affiliation: Department of Immunology, CNRS UMR 7276, CRIBL, Université de Limoges, Limoges, France.
authors.forename: Yves
authors.initials: Y
authors.lastname: Denizot
mesh_terms: D000483:Alleles; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology; D005260:Female; D006099:Granuloma / Q000473:pathology*; D006579:Heterozygote; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007249:Inflammation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D012333:RNA, Messenger / Q000378:metabolism; D013154:Spleen / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D007143:Immunoglobulin Heavy Chains; D012333:RNA, Messenger
keywords: IgH 3′ regulatory region; granulomas; knock-out mice; pristine