title: Effects of Acute Endurance Exercise on Plasma Protein Profiles of Endurance-Trained and Untrained Individuals over Time.
abstract: Acute physical exercise and repeated exercise stimuli affect whole-body metabolic and immunologic homeostasis. The aim of this study was to determine plasma protein profiles of trained (EET, n = 19) and untrained (SED, n = 17) individuals at rest and in response to an acute bout of endurance exercise. Participants completed a bicycle exercise test at an intensity corresponding to 80% of their VO2max. Plasma samples were taken before, directly after, and three hours after exercise and analyzed using multiplex immunoassays. Seventy-eight plasma variables were included in the final analysis. Twenty-nine variables displayed significant acute exercise effects in both groups. Seven proteins differed between groups, without being affected by acute exercise. Among these A2Macro and IL-5 were higher in EET individuals while leptin showed elevated levels in SED individuals. Fifteen variables revealed group and time differences with elevated levels for IL-3, IL-7, IL-10, and TNFR2 in EET individuals. An interaction effect could be observed for nine variables including IL-6, MMP-2, MMP-3, and muscle damage markers. The proteins that differ between groups indicate a long-term exercise effect on plasma protein concentrations. These findings might be of importance in the development of exercise-based strategies in the prevention and therapy of chronic metabolic and inflammatory diseases and for training monitoring.
authors.affiliation: Department of Sports Medicine, Justus-Liebig University Giessen, Kugelberg 62, 35394 Giessen, Germany.
authors.forename: Marius
authors.initials: M
authors.lastname: Schild
authors.affiliation: Mathematical Institute, Justus-Liebig University Giessen, Arndtstraße 2, 35392 Giessen, Germany.
authors.forename: Gerrit
authors.initials: G
authors.lastname: Eichner
authors.affiliation: Department of Sports Medicine, University Hospital of Tuebingen, Hoppe-Seyler-Straße 6, 72076 Tuebingen, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Beiter
authors.affiliation: Division of Sport and Rehabilitation Medicine, University Hospital Ulm, Parkstraße 11, 89075 Ulm, Germany.
authors.forename: Martina
authors.initials: M
authors.lastname: Zügel
authors.affiliation: Department of Sports Medicine, Justus-Liebig University Giessen, Kugelberg 62, 35394 Giessen, Germany.
authors.forename: Ilke
authors.initials: I
authors.lastname: Krumholz-Wagner
authors.affiliation: Department of Sports Medicine, University Hospital of Tuebingen, Hoppe-Seyler-Straße 6, 72076 Tuebingen, Germany.
authors.forename: Jens
authors.initials: J
authors.lastname: Hudemann
authors.affiliation: Department of Sports Medicine, Justus-Liebig University Giessen, Kugelberg 62, 35394 Giessen, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Pilat
authors.affiliation: Department of Sports Medicine, Justus-Liebig University Giessen, Kugelberg 62, 35394 Giessen, Germany.
authors.forename: Karsten
authors.initials: K
authors.lastname: Krüger
authors.affiliation: Department of Sports Medicine, University Hospital of Tuebingen, Hoppe-Seyler-Straße 6, 72076 Tuebingen, Germany.
authors.forename: Andreas M
authors.initials: AM
authors.lastname: Niess
authors.affiliation: Division of Sport and Rehabilitation Medicine, University Hospital Ulm, Parkstraße 11, 89075 Ulm, Germany.
authors.forename: Jürgen M
authors.initials: JM
authors.lastname: Steinacker
authors.affiliation: Department of Sports Medicine, Justus-Liebig University Giessen, Kugelberg 62, 35394 Giessen, Germany.
authors.forename: Frank C
authors.initials: FC
authors.lastname: Mooren
mesh_terms: D000328:Adult; D001798:Blood Proteins / Q000378:metabolism*; D015444:Exercise / Q000502:physiology*; D006801:Humans; D016753:Interleukin-10 / Q000097:blood; D007377:Interleukin-3 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D015851:Interleukin-7 / Q000097:blood; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000097:blood; D019278:Matrix Metalloproteinase 3 / Q000097:blood; D047889:Receptors, Tumor Necrosis Factor, Type II / Q000097:blood; D055815:Young Adult
chemical_list: D001798:Blood Proteins; D007377:Interleukin-3; D015850:Interleukin-6; D015851:Interleukin-7; D047889:Receptors, Tumor Necrosis Factor, Type II; D016753:Interleukin-10; D019278:Matrix Metalloproteinase 3; D020778:Matrix Metalloproteinase 2