title: Development of nanoformulation of picroliv isolated from Picrorrhiza kurroa.
abstract: Picroliv, a mixture of picroside I and kutkoside isolated from rhizome of Picrorrhiza kurroa has been reported for many pharmaceutical properties such as hepatoprotective, anticholestatic, antioxidant and immune-modulating activity. However, picroliv possessed lesser efficacy due to its poor aqueous solubility and lesser bioavailability. To find solution, picroliv was loaded into biodegradable poly lactic acid nanoparticles (PLA NPs) using solvent evaporation method. The picroliv-loaded PLA NPs were characterised by UV-vis spectroscopy, atomic force microscopy, transmission electron microscopy, Fourier transform infrared and Zeta sizer. The size of picroliv-loaded PLA NPs was 182 ± 20 nm. Zeta potential of picroliv-loaded PLA NPs was -23.5 mV, indicated their good stability. In vitro picroliv release from picroliv-loaded PLA NPs showed an initial burst release followed by slow and sustained release. The efficacy of picroliv-loaded PLA NPs was assessed against KB cell lines. Blank PLA NPs showed no cytotoxicity on KB cells. The picroliv-loaded PLA NPs showed more cytotoxic activity on KB cells as compared to the pure drug. Hence, the developed picroliv nanoformulation would find potential application in pharma-sector.
authors.affiliation: Acedemy of Scientific and Innovative Research, New Delhi, India.
authors.forename: Anika
authors.initials: A
authors.lastname: Guliani
authors.affiliation: Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur-176061, HP, India.
authors.forename: Avnesh
authors.initials: A
authors.lastname: Kumari
authors.affiliation: Food Nutraceuticals and Quality Control Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur-176061, HP, India.
authors.forename: Dharmesh
authors.initials: D
authors.lastname: Kumar
authors.affiliation: Acedemy of Scientific and Innovative Research, New Delhi, India. skyt@rediffmail.com.
authors.forename: Sudesh Kumar
authors.initials: SK
authors.lastname: Yadav
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D002934:Cinnamates / Q000737:chemistry* / Q000302:isolation & purification / Q000493:pharmacokinetics* / Q000633:toxicity; D004337:Drug Carriers / Q000737:chemistry* / Q000633:toxicity; D006027:Glycosides / Q000737:chemistry* / Q000302:isolation & purification / Q000493:pharmacokinetics* / Q000633:toxicity; D006801:Humans; D007624:KB Cells; D019344:Lactic Acid / Q000737:chemistry / Q000633:toxicity; D053758:Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D032261:Picrorhiza / Q000737:chemistry*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry / Q000633:toxicity; D014641:Vanillic Acid / Q000737:chemistry* / Q000302:isolation & purification / Q000493:pharmacokinetics* / Q000633:toxicity
chemical_list: D002934:Cinnamates; D004337:Drug Carriers; D006027:Glycosides; D011091:Polyesters; D011108:Polymers; C057463:kutkin; D019344:Lactic Acid; C033616:poly(lactide); D014641:Vanillic Acid