title: Regulation of Tissue-Specific Alternative Splicing: C. elegans as a Model System.
abstract: Alternative pre-mRNA splicing serves as an elegant mechanism for generating transcriptomic and proteomic diversity between cell and tissue types. In this chapter, we highlight key concepts and outstanding goals in studies of tissue and cell-specific alternative splicing. We place particular emphasis on the use of C. elegans as a tractable model organism for in vivo studies of alternative splicing between tissues and also at single cell resolution. We describe our current understanding of tissue and cell-specific regulation in the animal, and emerging techniques that will allow for future mechanistic studies as well as systems level investigations of spatio-temporal splicing under laboratory conditions and in response to environmental stimuli.
authors.affiliation: FAS Center for Systems Biology, Harvard University, 52 Oxford Street, Cambridge, MA, 02138, USA.
authors.forename: Xicotencatl
authors.initials: X
authors.lastname: Gracida
authors.affiliation: FAS Center for Systems Biology, Harvard University, 52 Oxford Street, Cambridge, MA, 02138, USA.
authors.forename: Adam D
authors.initials: AD
authors.lastname: Norris
authors.affiliation: FAS Center for Systems Biology, Harvard University, 52 Oxford Street, Cambridge, MA, 02138, USA. jcalarco@fas.harvard.edu.
authors.forename: John A
authors.initials: JA
authors.lastname: Calarco
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D005075:Biological Evolution; D017173:Caenorhabditis elegans / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins / Q000502:physiology*; D005091:Exons; D005786:Gene Expression Regulation / Q000235:genetics*; D017930:Genes, Reporter; D006801:Humans; D007448:Invertebrates / Q000235:genetics; D009928:Organ Specificity; D012313:RNA / Q000235:genetics / Q000378:metabolism; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism; D000072260:RNA Splicing Factors / Q000502:physiology*; D018510:RNA, Helminth / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000502:physiology; D013045:Species Specificity; D017381:Spliceosomes / Q000378:metabolism; D014714:Vertebrates / Q000235:genetics
chemical_list: D029742:Caenorhabditis elegans Proteins; D012322:RNA Precursors; D000072260:RNA Splicing Factors; D018510:RNA, Helminth; D012333:RNA, Messenger; C039919:RNA, recombinant; D016601:RNA-Binding Proteins; D012313:RNA
keywords: Alternative splicing; C. elegans; RNA processing; Tissue-specific regulation