title: Transcriptomic Analysis of Adult Renal Derived Mesenchymal Stem-Like Cells.
abstract: Mesenchymal stem cells (MSC) from bone marrow or adult tissues are widely studied to evaluate their potential for tissue repair. Differences in tissue of origin, donor variation, or in vitro handling exist and it is still unclear how they affect cell function and regenerative potential. Large-scale gene expression analysis of these cells not only allows researchers to compare and contrast the differences between each MSC subset but also allows for the development of better analytical tools for their characterization and utilization. Here, we describe a protocol for transcriptomics analysis of MSC-like cells derived from adult kidneys.
authors.affiliation: Division of Cardiology, Department of Medicine, Duke University Medical Center & Duke Cardiovascular Research Center, Durham, NC, 27710, USA.
authors.forename: Jose
authors.initials: J
authors.lastname: Gomez
authors.affiliation: Division of Cardiology, Department of Medicine, Duke University Medical Center & Duke Cardiovascular Research Center, Durham, NC, 27710, USA.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Schmeckpeper
authors.affiliation: Division of Cardiology, Department of Medicine, Duke University Medical Center & Duke Cardiovascular Research Center, Durham, NC, 27710, USA. mmirotsou@capricor.com.
authors.forename: Maria
authors.initials: M
authors.lastname: Mirotsou
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D002469:Cell Separation; D002478:Cells, Cultured; D015925:Cryopreservation; D020869:Gene Expression Profiling / Q000379:methods*; D007668:Kidney / Q000166:cytology* / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice
keywords: In vitro characterization; RNA analysis; Renal MSC-like cells