title: Proteinuria and lipoprotein lipase activity in Miniature Schnauzer dogs with and without hypertriglyceridemia.
abstract: Spontaneous hyperlipidemia in rats causes glomerular disease. Idiopathic hypertriglyceridemia (HTG) is prevalent in Miniature Schnauzers, but its relationship with proteinuria is unknown. Decreased activity of major lipid metabolism enzymes, lipoprotein lipase (LPL) and hepatic lipase (HL), may play a role in the cyclic relationship between hyperlipidemia and proteinuria. These enzymes have also not been previously investigated in Miniature Schnauzers. The aims of this study were to determine the relationship between HTG and proteinuria in Miniature Schnauzers and to measure LPL and HL activities in a subset of dogs. Fifty-seven Miniature Schnauzers were recruited (34 with and 23 without HTG). Fasting serum triglyceride concentrations and urine protein-to-creatinine ratios (UPC) were measured in all dogs, and LPL and HL activities were determined in 17 dogs (8 with and 9 without HTG). There was a strong positive correlation between triglyceride concentration and UPC (r = 0.77-0.83, P < 0.001). Proteinuria (UPC ≥ 0.5) was present in 60% of dogs with HTG and absent from all dogs without HTG (P < 0.001). Proteinuric dogs were not azotemic or hypoalbuminemic. Dogs with HTG had a 65% reduction in LPL activity relative to dogs without HTG (P < 0.001); HL activity did not differ. Proteinuria occurs with HTG in Miniature Schnauzers and could be due to lipid-induced glomerular injury. Reduced LPL activity may contribute to the severity of HTG, but further assay validation is required.
authors.affiliation: Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55113, USA. Electronic address: furro004@umn.edu.
authors.forename: E
authors.initials: E
authors.lastname: Furrow
authors.affiliation: Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: J Q
authors.initials: JQ
authors.lastname: Jaeger
authors.affiliation: Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: V J
authors.initials: VJ
authors.lastname: Parker
authors.affiliation: Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: K W
authors.initials: KW
authors.lastname: Hinchcliff
authors.affiliation: Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: S E
authors.initials: SE
authors.lastname: Johnson
authors.affiliation: Division of Metabolism, Endocrinology and Nutrition, Department of Medicine, University of Washington, Seattle, WA 98195, USA.
authors.forename: S J
authors.initials: SJ
authors.lastname: Murdoch
authors.affiliation: Division of Nephrology, Department of Medicine, University of Washington, Seattle, WA 98104, USA.
authors.forename: I H
authors.initials: IH
authors.lastname: de Boer
authors.affiliation: Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: R G
authors.initials: RG
authors.lastname: Sherding
authors.affiliation: Division of Metabolism, Endocrinology and Nutrition, Department of Medicine, University of Washington, Seattle, WA 98195, USA.
authors.forename: J D
authors.initials: JD
authors.lastname: Brunzell
mesh_terms: D000818:Animals; D003404:Creatinine / Q000097:blood; D004283:Dog Diseases; D004285:Dogs; D005260:Female; D015228:Hypertriglyceridemia / Q000378:metabolism / Q000662:veterinary*; D008071:Lipoprotein Lipase / Q000172:deficiency / Q000378:metabolism*; D008297:Male; D008910:Minnesota; D009820:Ohio; D011507:Proteinuria / Q000378:metabolism / Q000662:veterinary*; D013045:Species Specificity; D014280:Triglycerides / Q000097:blood*
chemical_list: D014280:Triglycerides; D003404:Creatinine; D008071:Lipoprotein Lipase
keywords: Canine; Glomerular disease; Hyperlipidemia; Miniature Schnauzer; Triglycerides