title: [Effect of hydrogen sulfide on cardiac myosin light chain kinase expression in diabetic rats].
abstract: OBJECTIVE
  To investigate the effect of hydrogen sulfide (H2S) on cardiac myosin light chain kinase (MLCK) expression in diabetic rats.
  
  
  METHODS
  A total of 32 male SD rats were randomly divided into a normal control group (NC group), a diabetic control group (DM), a NaHS treatment group (DM+NaHS) and a NaHS group (NaHS) (n=8 in each group). Intraperitoneal injection of streptozotocin was utilized to establish Type 1 diabetic rat model. The diabetic rats in the DM+NaHS and NaHS groups were intraperitoneally injected with 28 μmol/kg NaHS solution. Eight weeks later, the ventricular hemodynamic parameters, the ratio of heart weight/body weight (HW/BW ratio), the levels of lactate dehydrogenase (LDH) and creatine kinase MB isozyme (CK-MB) in serum were determined. The ultrastructures of myocardium were observed under electron microscopy. The expressions of MLCK mRNA and protein level in myocardium were detected by RT-PCR and Western blot, respectively.
  
  
  RESULTS
  Compared with the NC group, there was no significant difference in the various indexes in the NaHS group (all P>0.05). The function of left ventricular contract and relaxation were decreased obviously in diabetic rats, while the HW/BW ratio was increased (all P<0.01). The levels of LDH and CK-MB were increased (both P<0.01) in serum, while the levels of MLCK mRNA and protein were decreased significantly (both P<0.01) in myocardial tissues. Compared with the DM group, the left ventricular hemodynamic parameters and myocardial ultrastructure damage were improved in the DM+NaHS group, while the HW/BW ratio was decreased (all P<0.05). The levels of LDH and CK-MB were decreased (both P<0.01), while the levels of MLCK mRNA and protein were increased significantly (both P<0.01).
  
  
  CONCLUSIONS
  H2S can protect myocardium in diabetic rats, which may be associated with upregulation of cardiac MLCK.
authors.affiliation: Department of Physiology, Bengbu Medical College, Bengbu Anhui 233030, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Yang
authors.affiliation: Department of Physiology.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Jia
authors.affiliation: Department of Physiology.
authors.forename: Xiaofen
authors.initials: X
authors.lastname: Liu
authors.affiliation: Center of Functional Experiment, Bengbu Medical College, Bengbu Anhui 233030, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Physiology.
authors.forename: Qin
authors.initials: Q
authors.lastname: Gao
authors.affiliation: Department of Physiology.
authors.forename: Shanfeng
authors.initials: S
authors.lastname: Ma
mesh_terms: D000818:Animals; D002316:Cardiotonic Agents / Q000494:pharmacology*; D052279:Creatine Kinase, MB Form / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D006321:Heart / Q000187:drug effects; D006439:Hemodynamics; D006862:Hydrogen Sulfide / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D009206:Myocardium / Q000648:ultrastructure; D009219:Myosin-Light-Chain Kinase / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013440:Sulfides / Q000494:pharmacology; D016277:Ventricular Function, Left / Q000187:drug effects
chemical_list: D002316:Cardiotonic Agents; D013440:Sulfides; D007770:L-Lactate Dehydrogenase; D009219:Myosin-Light-Chain Kinase; D052279:Creatine Kinase, MB Form; C025451:sodium bisulfide; D006862:Hydrogen Sulfide