title: Third-harmonic generation imaging of breast tissue biopsies.
abstract: We demonstrate for the first time the imaging of unstained breast tissue biopsies using third-harmonic generation (THG) microscopy. As a label-free imaging technique, THG microscopy is compared to phase contrast and polarized light microscopy which are standard imaging methods for breast tissues. A simple feature detection algorithm is applied to detect tumour-associated lymphocyte rich regions in unstained breast biopsy tissue and compared with corresponding regions identified by a pathologist from bright-field images of hematoxylin and eosin stained breast tissue. Our results suggest that THG imaging holds potential as a complementary technique for analysing breast tissue biopsies.
authors.affiliation: Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, U.S.A.
authors.forename: Woowon
authors.initials: W
authors.lastname: Lee
authors.affiliation: Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, U.S.A.
authors.forename: Mohammad M
authors.initials: MM
authors.lastname: Kabir
authors.affiliation: Department of Pathology, University of Illinois at Chicago, Chicago, Illinois, U.S.A.
authors.forename: Rajyasree
authors.initials: R
authors.lastname: Emmadi
authors.affiliation: Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, U.S.A.. ktoussai@illinois.edu.|Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign and Bioengineering, Urbana, Illinois, U.S.A.. ktoussai@illinois.edu.
authors.forename: Kimani C
authors.initials: KC
authors.lastname: Toussaint
mesh_terms: D001706:Biopsy; D001940:Breast / Q000000981:diagnostic imaging* / Q000473:pathology*; D005260:Female; D006801:Humans; D008853:Microscopy / Q000379:methods*; D046888:Tissue Array Analysis
keywords: Breast cancer; breast tissue biopsy; nonlinear microscopy; third-harmonic generation; tissue microarrays; tumour-associated lymphocytes