title: Myeloperoxidase-responsive materials for infection detection based on immobilized aminomethoxyphenol.
abstract: There is a strong need for simple and fast diagnostic tools for the detection of wound infection. Immune system-derived enzymes like myeloperoxidase are efficient biomarkers for wound infection that emerge in the early stage infection process. In this study, 5-amino-2-methoxyphenol was functionalized with alkoxysilane to allow visual detection of MPO on carrier materials, for example, in test strips. Indeed, MPO activity was visually detectable in short time in wound background. Oxidation of the substrate was followed spectrophotometrically and proved via HPLC. LC-ESI TOF and NMR analyses unveiled the reaction mechanism and a dimeric reaction product responsible for the visualization of MPO activity. The substrate specificity and sensitivity toward MPO detection was proved and tests with infected wound fluids were successfully performed. The study demonstrates the suitability of the novel MPO substrate for the detection of wound infection and the covalent immobilization on diagnostic carrier materials. Biotechnol. Bioeng. 2016;113: 2553-2560. © 2016 Wiley Periodicals, Inc.
authors.affiliation: ACIB-Austrian Centre of Industrial Biotechnology, Tulln, Lower Austria, Austria.
authors.forename: Doris
authors.initials: D
authors.lastname: Schiffer
authors.affiliation: Institute of Environmental Biotechnology, University of Natural Recourses and Life Sciences Vienna, Konrad Lorenz Straße 20, Tulln an der Donau, 3430, Lower Austria, Austria. gregor.tegl@boku.ac.at.
authors.forename: Gregor
authors.initials: G
authors.lastname: Tegl
authors.affiliation: Institute of Environmental Biotechnology, University of Natural Recourses and Life Sciences Vienna, Konrad Lorenz Straße 20, Tulln an der Donau, 3430, Lower Austria, Austria.
authors.forename: Robert
authors.initials: R
authors.lastname: Vielnascher
authors.affiliation: Department of Organic Chemistry, Technical University Graz, Graz, Styria, Austria.
authors.forename: Hansjoerg
authors.initials: H
authors.lastname: Weber
authors.affiliation: Institute of Environmental Biotechnology, University of Natural Recourses and Life Sciences Vienna, Konrad Lorenz Straße 20, Tulln an der Donau, 3430, Lower Austria, Austria.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Herrero-Rollett
authors.affiliation: Qualizyme GmbH, Graz, Styria, Austria.
authors.forename: Eva
authors.initials: E
authors.lastname: Sigl
authors.affiliation: Qualizyme GmbH, Graz, Styria, Austria.
authors.forename: Andrea
authors.initials: A
authors.lastname: Heinzle
authors.affiliation: ACIB-Austrian Centre of Industrial Biotechnology, Tulln, Lower Austria, Austria.|Institute of Environmental Biotechnology, University of Natural Recourses and Life Sciences Vienna, Konrad Lorenz Straße 20, Tulln an der Donau, 3430, Lower Austria, Austria.
authors.forename: Georg M
authors.initials: GM
authors.lastname: Guebitz
mesh_terms: D000327:Adsorption; D001672:Biocompatible Materials / Q000737:chemistry; D015415:Biomarkers / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods; D003124:Colorimetry / Q000379:methods*; D006139:Guaiacol / Q000737:chemistry*; D006801:Humans; D009195:Peroxidase / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014946:Wound Infection / Q000175:diagnosis* / Q000378:metabolism*
chemical_list: D001672:Biocompatible Materials; D015415:Biomarkers; D006139:Guaiacol; D009195:Peroxidase
keywords: biomarker; chronic wounds; color change; diagnostics; enzymes; immobilization