title: Contribution of the Ade Resistance-Nodulation-Cell Division-Type Efflux Pumps to Fitness and Pathogenesis of Acinetobacter baumannii.
abstract: Overexpression of chromosomal resistance-nodulation-cell division (RND)-type efflux systems with broad substrate specificity contributes to multidrug resistance (MDR) in Acinetobacter baumannii We have shown that modulation of expression of the structural genes for the efflux systems AdeABC and AdeIJK confers MDR and results in numerous alterations of membrane-associated cellular functions, in particular biofilm formation. However, the contribution of these RND pumps to cell fitness and virulence has not yet been studied. The biological cost of an antibiotic resistance mechanism is a key parameter in determining its stability and dissemination. From an entirely sequenced susceptible clinical isolate, we have generated a set of isogenic derivatives having single point mutations resulting in overexpression of each efflux system or with every pump deleted by allelic replacement. We found that overproduction of the pumps results in a significant decrease in fitness of the bacterial host when measured by competition experiments in vitro Fitness and virulence were also evaluated in vivo both in systemic and pulmonary infection models in immunocompetent mice. A diminished competitiveness of the AdeABC-overexpressing mutant was observed only after intraperitoneal inoculation, but not after intranasal inoculation, the latter mimicking the most frequent type of human A. baumannii infection. However, in mice infected intranasally, this mutant was more virulent and stimulated an enhanced neutrophil activation in the lungs. Altogether, these data account for the observation that adeABC overexpression is common in MDR A. baumannii frequently found in ventilator-associated pneumonia.
  
  
  
  Overproduction of the RND AdeABC efflux system is observed with a high incidence in multidrug-resistant Acinetobacter baumannii and results in increased resistance to several antibiotics of choice for the treatment of infections caused by this nosocomial pathogen. It was therefore important to study the biological cost of the overexpression of the adeABC structural operon which is normally tightly regulated. Fitness diminution of an overexpressing mutant detected in vitro and in vivo in a model that mimics sepsis was not observed in a pulmonary infection model in which the mutant was more virulent. This points out that increased virulence can occur independently from prolonged persistence in the infected organ and can account for the elevated incidence of this resistance mechanism in clinical isolates. The study also indicates that transposon libraries will identify only virulence genes that are expressed under physiological conditions but not those that are tightly regulated.
authors.affiliation: Institut Pasteur, Unité des Agents Antibactériens, Paris, France.
authors.forename: Eun-Jeong
authors.initials: EJ
authors.lastname: Yoon
authors.affiliation: INSERM, UMR S 938, CDR Saint-Antoine, Paris, France Sorbonne Université, UPMC, CDR Saint-Antoine, Paris, France.
authors.forename: Viviane
authors.initials: V
authors.lastname: Balloy
authors.affiliation: Institut Pasteur Unité Histopathologie Humaine et Modèles Animaux, Paris, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Fiette
authors.affiliation: INSERM, UMR S 938, CDR Saint-Antoine, Paris, France Sorbonne Université, UPMC, CDR Saint-Antoine, Paris, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Chignard
authors.affiliation: Institut Pasteur, Unité des Agents Antibactériens, Paris, France.
authors.forename: Patrice
authors.initials: P
authors.lastname: Courvalin
authors.affiliation: Institut Pasteur, Unité des Agents Antibactériens, Paris, France catherine.grillot-courvalin@pasteur.fr.
authors.forename: Catherine
authors.initials: C
authors.lastname: Grillot-Courvalin
mesh_terms: D000151:Acinetobacter Infections / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism / Q000472:pathogenicity*; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D018441:Biofilms / Q000254:growth & development; D002455:Cell Division / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D056084:Genetic Fitness; D006801:Humans; D007121:Immunocompetence; D008168:Lung / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D026901:Membrane Transport Proteins / Q000235:genetics*; D051379:Mice; D009504:Neutrophils / Q000276:immunology; D014774:Virulence
chemical_list: C492436:AdeA protein, Acinetobacter baumannii; C445684:AdeB protein, Acinetobacter baumannii; C492437:AdeC protein, Acinetobacter baumannii; D001426:Bacterial Proteins; D026901:Membrane Transport Proteins