title: Molecular, Cellular, and Physiological Characterization of Sirtuin 7 (SIRT7).
abstract: Sirtuin 7 (SIRT7), a histone 3 lysine 18 (H3K18) deacetylase, functions at chromatin to suppress endoplasmic reticulum (ER) stress and mitochondrial protein folding stress (PFS(mt)), and prevent the development of fatty liver disease and hematopoietic stem cell aging. In this chapter, we provide a methodology to characterize the molecular, cellular, and physiological functions of SIRT7.
authors.affiliation: Program in Metabolic Biology, Nutritional Sciences & Toxicology, University of California, Berkeley, CA, 94720, USA.
authors.forename: Jiyung
authors.initials: J
authors.lastname: Shin
authors.affiliation: Program in Metabolic Biology, Nutritional Sciences & Toxicology, University of California, Berkeley, CA, 94720, USA. danicac@berkeley.edu.
authors.forename: Danica
authors.initials: D
authors.lastname: Chen
mesh_terms: D016922:Cellular Senescence; D002843:Chromatin / Q000378:metabolism; D003001:Cloning, Molecular / Q000379:methods*; D059865:Endoplasmic Reticulum Stress; D006412:Hematopoietic Stem Cells / Q000502:physiology; D006801:Humans; D047468:Immunoprecipitation; D050356:Lipid Metabolism; D008928:Mitochondria / Q000378:metabolism; D011485:Protein Binding; D017510:Protein Folding; D037761:Sirtuins / Q000235:genetics* / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D002843:Chromatin; C510661:SIRT7 protein, human; D014157:Transcription Factors; D037761:Sirtuins
keywords: Aging; ER UPR (UPRer); ER stress; Fatty liver; Hematopoietic stem cell; Mitochondrial UPR (UPRmt); Mitochondrial protein folding stress; Myc; NRF1; SIRT7