title: Controlling mesenchymal stem cells differentiate into contractile smooth muscle cells on a TiO2 micro/nano interface: Towards benign pericytes environment for endothelialization.
abstract: Building healthy and oriented smooth muscle cells (SMCs) environment is an effective method for improving the surface endothelialization of the cardiovascular implants. However, a long-term and stable source of SMCs for implantation without immune rejection and inflammation has not been solved, and mesenchymal stem cells (MSCs) differentiation may be a good choice. In this work, two types of TiO2 micro/nano interfaces were fabricated on titanium surface by photolithography and anodic oxidation. These TiO2 micro/nano interfaces were used to regulate the differentiation of the MSCs. The X-ray diffraction (XRD) detection showed that the TiO2 micro/nano interfaces possessed the anatase crystal structure, suggesting good cytocompatibility. The CCK-8 results indicated the TiO2 micro/nano interfaces improved MSC proliferation, further immunofluorescence staining and calculation of the cell morphology index proved the micro/nano surfaces also elongated MSCs and regulated MSCs oriented growth. The specific staining of α-SMA, CNN-1, vWF, CD44 and CD133 markers revealed that the micro/nano surfaces induced MSCs differentiation to contractile SMCs, and the endothelial cells (ECs) culture experiment indicated that the MSCs induced by micro/nano interfaces contributed to the ECs attachment and proliferation. This method will be further studied and applied for the surface modification of the cardiovascular implants.
authors.affiliation: Key Lab. for Advanced Technologies of Materials, Ministry of Education, School of Material Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China. Electronic address: 4828713@qq.com.
authors.forename: Jingan
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Lab. for Advanced Technologies of Materials, Ministry of Education, School of Material Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China; Heze Alliancells Bioscience Co., Ltd., Changzhou 213000, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qin
authors.affiliation: Key Lab. for Advanced Technologies of Materials, Ministry of Education, School of Material Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China; School of Life Science, Zhengzhou University, 100 Science Road, Zhengzhou 450001, PR China; Center of Stem Cell and Regenerative Medicine, First Affiliated Hospital of Zhengzhou University, 40 University Road, Zhengzhou 450052, PR China.
authors.forename: Kun
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Key Lab. for Advanced Technologies of Materials, Ministry of Education, School of Material Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wu
authors.affiliation: Key Lab. for Advanced Technologies of Materials, Ministry of Education, School of Material Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China. Electronic address: yangping8@263.net.
authors.forename: Ping
authors.initials: P
authors.lastname: Yang
authors.affiliation: Key Lab. for Advanced Technologies of Materials, Ministry of Education, School of Material Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China.
authors.forename: Zikun
authors.initials: Z
authors.lastname: He
authors.affiliation: Key Lab. for Advanced Technologies of Materials, Ministry of Education, School of Material Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China.
authors.forename: Ansha
authors.initials: A
authors.lastname: Zhao
authors.affiliation: Key Lab. for Advanced Technologies of Materials, Ministry of Education, School of Material Science and Engineering, Southwest Jiaotong University, Chengdu 610031, PR China.
authors.forename: Nan
authors.initials: N
authors.lastname: Huang
mesh_terms: D000071916:AC133 Antigen / Q000378:metabolism; D000199:Actins / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D008840:Microfilament Proteins / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000187:drug effects; D020286:Pericytes / Q000166:cytology / Q000187:drug effects; D014025:Titanium / Q000737:chemistry*; D014961:X-Ray Diffraction; D014841:von Willebrand Factor / Q000378:metabolism; D000096985:Calponins
chemical_list: D000071916:AC133 Antigen; D000199:Actins; D002135:Calcium-Binding Proteins; D018960:Hyaluronan Receptors; D008840:Microfilament Proteins; D014025:Titanium; D014841:von Willebrand Factor; D000096985:Calponins; C541116:ACTA2 protein, human; C009495:titanium dioxide
keywords: Contractile smooth muscle cell; Differentiation; Mesenchymal stem cell; Micro-patterning; Nano topography