title: Isolation of yeast candidates for efficient sophorolipids production: their production potentials associate to their lineage.
abstract: Eleven biosurfactant-producing strains were newly isolated from environmental samples using a drop-collapse assay and thin-layer chromatography (TLC). According to the TLC analysis, the separation patterns of the glycolipid spots of nine dominant strains corresponded to that of the sophorolipids produced by a Starmerella bombicola type strain. The retention factor values of the spot patterns of two strains were less than those of the others. Two representative major products were purified, and their molecular structures were determined. The major products were identified as diacetylated lactonic and acidic sophorolipids. The fatty acid moieties of both compounds were estimated to be 17-hydroxymethyl hexadecenoic acid. The amounts of glycolipids ranged from 5.0 to 22.9 g/L after 4 d of cultivation. According to a phylogenetic analysis, the strains were identified as Starmerella bombicola and Candida floricola.
authors.affiliation: a Department of Biotechnology and Environmental Chemistry , Kitami Institute of Technology , Kitami , Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Konishi
authors.affiliation: a Department of Biotechnology and Environmental Chemistry , Kitami Institute of Technology , Kitami , Japan.
authors.forename: Manako
authors.initials: M
authors.lastname: Fujita
authors.affiliation: a Department of Biotechnology and Environmental Chemistry , Kitami Institute of Technology , Kitami , Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Ishibane
authors.affiliation: a Department of Biotechnology and Environmental Chemistry , Kitami Institute of Technology , Kitami , Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Shimizu
authors.affiliation: a Department of Biotechnology and Environmental Chemistry , Kitami Institute of Technology , Kitami , Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Tsukiyama
authors.affiliation: a Department of Biotechnology and Environmental Chemistry , Kitami Institute of Technology , Kitami , Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Ishida
mesh_terms: D006017:Glycolipids / Q000096:biosynthesis*; D010802:Phylogeny; D004718:Saccharomycetales / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism*; D013501:Surface-Active Agents / Q000378:metabolism
chemical_list: D006017:Glycolipids; D013501:Surface-Active Agents
keywords: Candida floricola; Starmerella bombicola; biosurfactant; sophorolipids; yeast