title: A study of the effects of therapeutic doses of ionizing radiation in vitro on Lactobacillus isolates originating from the vagina - a pilot study.
abstract: BACKGROUND
  Ionizing radiation is used as a therapeutic option in the treatment of certain neoplastic lesions located, among others, in the pelvic region. The therapeutic doses of radiation employed often result in adverse effects manifesting themselves primarily in the form of genital tract infections in patients or diarrhea. The data available in the literature indicate disorders in the microbial ecosystem caused by ionizing radiation, which leads to the problems mentioned above. In the present study, we examined the influence of ionizing radiation on 52 selected strains of bacteria: Lactobacillus crispatus, L. fermentum, L. plantarum, L. reuteri, L. acidophilus L. amylovorus, L. casei, L. helveticus, L. paracasei, L. rhamnosus, L. salivarius and L. gasseri. This collection of Lactobacillus bacteria isolates of various species, obtained from the genital tract and gastrointestinal tract of healthy women, was tested for resistance to therapeutic doses of ionizing radiation.
  
  
  RESULTS
  The species studied, were isolated from the genital tract (n = 30) and from the anus (n = 22) of healthy pregnant women. Three doses of 3 Gy (fractionated dose) and 50 Gy (total dose of the whole radiotherapy cycle) were applied. The greatest differences in survival of the tested strains in comparison to the control group (not subjected to radiation) were observed at the dose of 50 Gy. However, the results were not statistically significant. Survival decrease to zero was not demonstrated for any of the tested strains.
  
  
  CONCLUSIONS
  Therapeutic doses of radiation do not affect the Lactobacillus bacteria significantly.
authors.affiliation: Department of Bacteriology, Microbial Ecology and Parasitology, Chair of Microbiology, Jagiellonian University Medical College, 18 Czysta Street, 31-121, Krakow, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Gosiewski
authors.affiliation: Institute of Biology, Pedagogical University, 2 Podchorazych Str, 30-084, Krakow, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Mróz
authors.affiliation: Department of Infection Epidemiology, Chair of Microbiology, Jagiellonian University Medical College, 18 Czysta Str, Krakow, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Ochońska
authors.affiliation: Department of Gynaecology and Endocrinology, University Hospital, 23 Kopernika Str., 31-501, Krakow, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Pabian
authors.affiliation: Department of Infection Epidemiology, Chair of Microbiology, Jagiellonian University Medical College, 18 Czysta Str, Krakow, Poland.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Bulanda
authors.affiliation: Department of Bacteriology, Microbial Ecology and Parasitology, Chair of Microbiology, Jagiellonian University Medical College, 18 Czysta Street, 31-121, Krakow, Poland. mbrzych@cm-uj.krakow.pl.
authors.forename: Monika
authors.initials: M
authors.lastname: Brzychczy-Wloch
mesh_terms: D004307:Dose-Response Relationship, Radiation; D005260:Female; D041981:Gastrointestinal Tract / Q000382:microbiology*; D064368:Healthy Volunteers; D006801:Humans; D007778:Lactobacillus / Q000145:classification / Q000302:isolation & purification* / Q000528:radiation effects*; D010865:Pilot Projects; D011829:Radiation Dosage; D011836:Radiation Tolerance; D011839:Radiation, Ionizing; D014621:Vagina / Q000382:microbiology*
keywords: Ionizing radiation; Lactobacillus; Microbial flora