title: Label-free imaging of metabolism and oxidative stress in human induced pluripotent stem cell-derived cardiomyocytes.
abstract: In this work we demonstrate a label-free optical imaging technique to assess metabolic status and oxidative stress in human induced pluripotent stem cell-derived cardiomyocytes by two-photon fluorescence lifetime imaging of endogenous fluorophores. Our results show the sensitivity of this method to detect shifts in metabolism and oxidative stress in the cardiomyocytes upon pathological stimuli of hypoxia and cardiotoxic drugs. This non-invasive imaging technique could prove beneficial for drug development and screening, especially for in vitro cardiac models created from stem cell-derived cardiomyocytes and to study the pathogenesis of cardiac diseases and therapy.
authors.affiliation: Laboratory for Fluorescence Dynamic, University of California, Biomedical Engineering, Irvine, California 92617, USA.
authors.forename: Rupsa
authors.initials: R
authors.lastname: Datta
authors.affiliation: Laboratory for Fluorescence Dynamic, University of California, Biomedical Engineering, Irvine, California 92617, USA.
authors.forename: Christopher
authors.initials: C
authors.lastname: Heylman
authors.affiliation: Washington University in St. Louis, Biomedical Engineering, St. Louis, Missouri, 63130, USA.
authors.forename: Steven C
authors.initials: SC
authors.lastname: George
authors.affiliation: Laboratory for Fluorescence Dynamic, University of California, Biomedical Engineering, Irvine, California 92617, USA.
authors.forename: Enrico
authors.initials: E
authors.lastname: Gratton
keywords: (170.3880) Medical and biological imaging; (180.2520) Fluorescence microscopy