title: [A NOVEL APPROACH TO GENOTYPING OF HOSPITAL ISOLATES OF CLOSTRIDIUM DIFFICILE].
abstract: OBJECTIVE
  Development of a novel approach in genotyping of Clostridium difficile and its testing on the example of 140 hospital isolates.
  
  
  METHODS
  The approach is based on an idea of double digest and selective label (DDSL), used previously during genotyping of other bacterial pathogens. Selection of optimal enzymes for restriction of MluI and Mph1103I was carried out, condition of DDSL reaction execution were optimized.
  
  
  RESULTS
  Genotyping of C. difficile hospital isolates was carried out, index of strain discrimination was calculated, conclusions regarding possibilities of the method in elucidation of spread pathways and identification of infection sources were made.
  
  
  CONCLUSIONS
  The developed method of genotyping has a number of advantages over the existing method and can be used to'address issues in epidemiology of infections caused by C. difficile.
authors.forename: V P
authors.initials: VP
authors.lastname: Terletsky
authors.forename: V I
authors.initials: VI
authors.lastname: Tyschenko
authors.forename: O B
authors.initials: OB
authors.lastname: Novikova
authors.forename: I I
authors.initials: II
authors.lastname: Novikova
authors.forename: E D
authors.initials: ED
authors.lastname: Dzhavadov
mesh_terms: D016360:Clostridioides difficile / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D003428:Cross Infection / Q000235:genetics / Q000382:microbiology*; D004269:DNA, Bacterial / Q000145:classification / Q000235:genetics*; D004761:Enterocolitis, Pseudomembranous / Q000235:genetics* / Q000382:microbiology; D005838:Genotype; D006761:Hospitals; D006801:Humans; D017720:Molecular Epidemiology
chemical_list: D004269:DNA, Bacterial