title: PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis.
abstract: Plant development mediated by the phytohormone auxin depends on tightly controlled cellular auxin levels at its target tissue that are largely established by intercellular and intracellular auxin transport mediated by PIN auxin transporters. Among the eight members of the Arabidopsis PIN family, PIN6 is the least characterized candidate. In this study we generated functional, fluorescent protein-tagged PIN6 proteins and performed comprehensive analysis of their subcellular localization and also performed a detailed functional characterization of PIN6 and its developmental roles. The localization study of PIN6 revealed a dual localization at the plasma membrane (PM) and endoplasmic reticulum (ER). Transport and metabolic profiling assays in cultured cells and Arabidopsis strongly suggest that PIN6 mediates both auxin transport across the PM and intracellular auxin homeostasis, including the regulation of free auxin and auxin conjugates levels. As evidenced by the loss- and gain-of-function analysis, the complex function of PIN6 in auxin transport and homeostasis is required for auxin distribution during lateral and adventitious root organogenesis and for progression of these developmental processes. These results illustrate a unique position of PIN6 within the family of PIN auxin transporters and further add complexity to the developmentally crucial process of auxin transport.
authors.affiliation: Institute of Science and Technology Austria (IST Austria), 3400, Klosterneuburg, Austria.|Department of Plant Systems Biology, VIB , 927, 9052, Zwijnaarde, Ghent, Belgium.|Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark, 927, 9052, Zwijnaarde, Ghent, Belgium.|CEITEC - Central European Institute of Technology, Masaryk University, 625 00, Brno, Czech Republic.
authors.forename: Sibu
authors.initials: S
authors.lastname: Simon
authors.affiliation: Institute of Experimental Botany, Czech Academy of Sciences, Rozvojová 263, 16502, Prague 6, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Skůpa
authors.affiliation: Department of Plant Systems Biology, VIB , 927, 9052, Zwijnaarde, Ghent, Belgium.|Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark, 927, 9052, Zwijnaarde, Ghent, Belgium.
authors.forename: Tom
authors.initials: T
authors.lastname: Viaene
authors.affiliation: CEITEC - Central European Institute of Technology, Masaryk University, 625 00, Brno, Czech Republic.
authors.forename: Marta
authors.initials: M
authors.lastname: Zwiewka
authors.affiliation: Department of Plant Systems Biology, VIB , 927, 9052, Zwijnaarde, Ghent, Belgium.|Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark, 927, 9052, Zwijnaarde, Ghent, Belgium.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Tejos
authors.affiliation: Institute of Experimental Botany, Czech Academy of Sciences, Rozvojová 263, 16502, Prague 6, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Klíma
authors.affiliation: Institute of Experimental Botany, Czech Academy of Sciences, Rozvojová 263, 16502, Prague 6, Czech Republic.
authors.forename: Mária
authors.initials: M
authors.lastname: Čarná
authors.affiliation: Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, 78371, Olomouc, Czech Republic.
authors.forename: Jakub
authors.initials: J
authors.lastname: Rolčík
authors.affiliation: Department of Plant Systems Biology, VIB , 927, 9052, Zwijnaarde, Ghent, Belgium.|Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark, 927, 9052, Zwijnaarde, Ghent, Belgium.
authors.forename: Riet
authors.initials: R
authors.lastname: De Rycke
authors.affiliation: Centro de Biotecnologia Vegetal and Center for Genome Regulation, Facultad de Ciencias Biologicas, Universidad Andres Bello, 8370146, Santiago, Chile.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Moreno
authors.affiliation: Institute of Experimental Botany, Czech Academy of Sciences, Rozvojová 263, 16502, Prague 6, Czech Republic.
authors.forename: Petre I
authors.initials: PI
authors.lastname: Dobrev
authors.affiliation: Centro de Biotecnologia Vegetal and Center for Genome Regulation, Facultad de Ciencias Biologicas, Universidad Andres Bello, 8370146, Santiago, Chile.
authors.forename: Ariel
authors.initials: A
authors.lastname: Orellana
authors.affiliation: Institute of Experimental Botany, Czech Academy of Sciences, Rozvojová 263, 16502, Prague 6, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Zažímalová
authors.affiliation: Institute of Science and Technology Austria (IST Austria), 3400, Klosterneuburg, Austria.
authors.forename: Jiří
authors.initials: J
authors.lastname: Friml
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D019143:Evolution, Molecular; D006706:Homeostasis; D007210:Indoleacetic Acids / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D010802:Phylogeny; D018517:Plant Roots / Q000254:growth & development / Q000378:metabolism; D030821:Plants, Genetically Modified
chemical_list: D029681:Arabidopsis Proteins; D007210:Indoleacetic Acids; D026901:Membrane Transport Proteins; C581623:PIN6 protein, Arabidopsis
keywords: PIN; auxin; endoplasmic reticulum (ER); lateral root; plasma membrane (PM)