title: [Teaching design and practice of human blood type traits in genetics comprehensive laboratory course].
abstract: Comprehensive laboratory courses, which enable students to aptly apply theoretic knowledge and master experiment skills, play an important role in the present educational reform of laboratory courses. We utilized human ABO blood type as the experimental subject, and designed the experiment--"Molecular Genotyping of Human ABO Blood Type and Analysis of Population Genetic Equilibrium". In the experiment, DNA in mucosal cells is extracted from students' saliva, and each student's genotype is identified using a series of molecular genetics technologies, including PCR amplification of target fragments, enzymatic digestion, and electrophoretic separation. Then, taking the whole class as an analogous Mendel population, a survey of genotype frequency of ABO blood type is conducted, followed with analyses of various population genetic parameters using Popgene. Through the open laboratory course, students can not only master molecular genetic experimental skills, but also improve their understanding of theoretic knowledge through independent design and optimization of molecular techniques. After five years of research and practice, a stable experimental system of molecular genetics has been established to identify six genotypes of ABO blood types, namely I(A)I(A), I(A)i, I(B)I(B), I(B)i, I(A)I(B) and ii. Laboratory courses of molecular and population genetics have been integrated by calculating the frequencies of the six genotypes and three multiple alleles and testing population genetic equilibrium. The goal of the open laboratory course with independent design and implementation by the students has been achieved. This laboratory course has proved effective and received good reviews from the students. It could be applied as a genetics laboratory course for the biology majors directly, and its ideas and methods could be promoted and applied to other biological laboratory courses.
authors.affiliation: College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhao
authors.affiliation: College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.
authors.forename: Dong-mei
authors.initials: DM
authors.lastname: Hu
authors.affiliation: Department of Forest Botany and Tree Physiology, Georg-August-University Gö
authors.forename: Da-de
authors.initials: DD
authors.lastname: Yu
authors.affiliation: College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.
authors.forename: Ming-liang
authors.initials: ML
authors.lastname: Dong
authors.affiliation: College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.
authors.forename: Ying-ming
authors.initials: YM
authors.lastname: Fan
authors.affiliation: College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.
authors.forename: Yan-wei
authors.initials: YW
authors.lastname: Wang
authors.affiliation: College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.
authors.forename: Jin-feng
authors.initials: JF
authors.lastname: Zhang
mesh_terms: D000017:ABO Blood-Group System / Q000235:genetics*; D005823:Genetics / Q000193:education*; D005838:Genotype; D006801:Humans; D013677:Medical Laboratory Science / Q000193:education*; D012107:Research Design; D013663:Teaching
chemical_list: D000017:ABO Blood-Group System