title: [Antitumor Chemical Entities of Cordyceps taii Mycelia Powder from Guizhou].
abstract: OBJECTIVE
  To seperate and identify the chemicals from the antitumor fraction of Cordyceps taii mycelia powder.
  
  
  METHODS
  The mycelia of Cordyceps taii were prepared by the submerged fermentation technique. Chemical entities in the antitumor fraction of Cordyceps taii were isolated and purified by using different column chromatographies (silica gel, Sephadex LH-20 and MCI), and semi-preparative HPLC method. Theirs chemical structures were then identified by different spectrum techniques such as EI, ESI and 1D/2D-NMR, etc. The cytotoxic activity was investigated by the Sulforhodamine B (SRB) assay.
  
  
  RESULTS
  Six compounds, such as 5α,8α-epidioxyergosta-6,22-dien-3β-ol (1), ergosterol (2), adenine nucleoside (3), helvolic acid (4), deacetylcytochalasin C (5) and zygosporin D (6), were identified. The IC50 value of compound 2 against human gastric cancer cell line SGC-7901 was 5.99 μmol/L, which was less than the half value of cisplatin, and had lower cytotoxicity to normal cells in comparison with cisplatin.
  
  
  CONCLUSIONS
  Six compounds have been isolated from the antitumor fraction of Cordyceps taii mycelia powder,of which compounds 1, 5 and 6 are isolated from Cordyceps taii for the first time. Compounds 1, 2 and 4 have cytotoxic activities against cancer cells, and should be the main antitumor compounds of Cordyceps taii.
authors.forename: Xiao-gang
authors.initials: XG
authors.lastname: Li
authors.forename: Wei-dong
authors.initials: WD
authors.lastname: Pan
authors.forename: Xiao-jie
authors.initials: XJ
authors.lastname: Zhang
authors.forename: Jian-hui
authors.initials: JH
authors.lastname: Xiao
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry*; D045744:Cell Line, Tumor / Q000187:drug effects; D002945:Cisplatin; D032661:Cordyceps / Q000737:chemistry*; D004875:Ergosterol / Q000031:analogs & derivatives / Q000737:chemistry; D005285:Fermentation; D005672:Fusidic Acid / Q000031:analogs & derivatives / Q000737:chemistry; D006801:Humans; D020128:Inhibitory Concentration 50; D025282:Mycelium / Q000737:chemistry; D011208:Powders; D013274:Stomach Neoplasms / Q000473:pathology*
chemical_list: C503471:5alpha,8alpha-epidioxyergosta-6,22-dien-3-beta-ol; D000970:Antineoplastic Agents; D011208:Powders; D005672:Fusidic Acid; C011267:helvolic acid; D002945:Cisplatin; D004875:Ergosterol