title: Reduced L-type Ca2+ current and compromised excitability induce loss of skeletal muscle function during acute cooling in locust.
abstract: Low temperature causes most insects to enter a state of neuromuscular paralysis, termed chill coma. The susceptibility of insect species to chill coma is tightly correlated to their distribution limits and for this reason it is important to understand the cellular processes that underlie chill coma. It is known that muscle function is markedly depressed at low temperature and this suggests that chill coma is partly caused by impairment in the muscle per se. To find the cellular mechanism(s) underlying muscle dysfunction at low temperature, we examined the effect of low temperature (5°C) on several events in excitation-contraction coupling in the migratory locust (Locusta migratoria). Intracellular membrane potential recordings during single nerve stimulations showed that 70% of fibers at 20°C produced an action potential (AP), while only 55% of fibers were able to fire an AP at 5°C. Reduced excitability at low temperature was caused by an ∼80% drop in L-type Ca(2+) current and a depolarizing shift in its activation of around 20 mV, which means that a larger endplate potential would be needed to activate the muscle AP at low temperature. In accordance, we showed that intracellular Ca(2+) transients were largely absent at low temperature following nerve stimulation. In contrast, maximum contractile force was unaffected by low temperature in chemically skinned muscle bundles, which demonstrates that the function of the contractile filaments is preserved at low temperature. These findings demonstrate that reduced L-type Ca(2+) current is likely to be the most important factor contributing to loss of muscle function at low temperature in locust.
authors.affiliation: Zoophysiology, Department of Bioscience, C.F. Møllers Allé 3, Building 1131, Aarhus University, 8000 Aarhus C, Denmark.
authors.forename: Anders
authors.identifier: 0000-0002-7011-3604
authors.initials: A
authors.lastname: Findsen
authors.affiliation: Zoophysiology, Department of Bioscience, C.F. Møllers Allé 3, Building 1131, Aarhus University, 8000 Aarhus C, Denmark.
authors.forename: Johannes
authors.initials: J
authors.lastname: Overgaard
authors.affiliation: Department of Biomedicine, Danish Biomembrane Research Centre, Ole Worms Allé 3, Building 1160, Aarhus University, 8000 Aarhus C, Denmark thp@biomed.au.dk.
authors.forename: Thomas Holm
authors.initials: TH
authors.lastname: Pedersen
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000818:Animals; D001696:Biomechanical Phenomena; D020746:Calcium Channels, L-Type / Q000378:metabolism*; D020013:Calcium Signaling; D003080:Cold Temperature; D004558:Electric Stimulation; D015640:Ion Channel Gating; D048188:Locusta migratoria / Q000502:physiology*; D009045:Motor Endplate / Q000502:physiology; D018482:Muscle, Skeletal / Q000502:physiology*
chemical_list: D020746:Calcium Channels, L-Type
keywords: Chill coma; Contractile force; Excitation–contraction coupling; Neuromuscular dysfunction