title: Macrophage Isolation from the Mouse Small and Large Intestine.
abstract: Macrophages play important roles in maintaining intestinal homeostasis via their ability to orchestrate responses to the normal microbiota as well as pathogens. One of the most important steps in beginning to understand the functions of these cells is the ability to effectively isolate them from the complex intestinal environment. Here, we detail methodology for the isolation and phenotypic characterization of macrophages from the mouse small and large intestine.
authors.affiliation: Institute for Biomedical Sciences, Georgia State University, Atlanta, GA, 30303, USA.
authors.forename: Akihito
authors.initials: A
authors.lastname: Harusato
authors.affiliation: Department of Pathology & Laboratory Medicine, Emory University, Atlanta, GA, 30322, USA.
authors.forename: Duke
authors.initials: D
authors.lastname: Geem
authors.affiliation: Institute for Biomedical Sciences, Georgia State University, 100 Piedmont Avenue, Room 690, Atlanta, GA, 30303, USA. tdenning@gsu.edu.
authors.forename: Timothy L
authors.initials: TL
authors.lastname: Denning
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000379:methods*; D005434:Flow Cytometry; D007420:Intestine, Large / Q000166:cytology; D007421:Intestine, Small / Q000166:cytology; D008264:Macrophages / Q000166:cytology*; D051379:Mice
keywords: Antigen-presenting cell; Dendritic cell; Intestine; Macrophage