title: Improvement on D-xylose to Xylitol Biotransformation by Candida guilliermondii Using Cells Permeabilized with Triton X-100 and Selected Process Conditions.
abstract: Cells of Candida guilliermondii permeabilized with Triton X-100 were able to efficiently produce xylitol from a medium composed only by D-xylose and MgCl2·6H2O in potassium phosphate buffer, at 35 °C and pH 6.5. Under these conditions, the results were similar to those obtained when cofactor and co-substrate or nutrients were added to the medium (about 95 % D-xylose was assimilated producing 42 g/L of xylitol, corresponding to 0.80 g/g yield and 2.65 g/L h volumetric productivity). Furthermore, the permeabilized cells kept the D-xylose assimilation in about 90 % and the xylitol production in approx. 40 g/L during three bioconversion cycles of 16 h each. These values are highly relevant when compared to others reported in the literature using enzyme technology and fermentative process, thereby demonstrating the effectiveness of the proposed method. The present study reveals that the use of permeabilized cells is an interesting alternative to obtain high xylitol productivity using low cost medium formulation. This approach may allow the future development of xylitol production from xylose present in lignocellulosic biomass, with additional potential for implementation in biorefinery strategies.
authors.affiliation: Departamento de Biotecnologia, Escola de Engenharia de Lorena, Universidade de São Paulo, CEP:12.602-810, Lorena, São Paulo, Brazil.
authors.forename: Daniela Vieira
authors.initials: DV
authors.lastname: Cortez
authors.affiliation: Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands.
authors.forename: Solange I
authors.initials: SI
authors.lastname: Mussatto
authors.affiliation: Departamento de Biotecnologia, Escola de Engenharia de Lorena, Universidade de São Paulo, CEP:12.602-810, Lorena, São Paulo, Brazil. ines@debiq.eel.usp.br.
authors.forename: Inês Conceição
authors.initials: IC
authors.lastname: Roberto
mesh_terms: D001709:Biotechnology / Q000379:methods*; D001711:Biotransformation / Q000187:drug effects; D002175:Candida / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D002463:Cell Membrane Permeability / Q000187:drug effects*; D003470:Culture Media / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D017830:Octoxynol / Q000494:pharmacology*; D013696:Temperature; D014993:Xylitol / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism*
chemical_list: D003470:Culture Media; D017830:Octoxynol; D014994:Xylose; D014993:Xylitol
keywords: Biotransformation; Candida guilliermondii; D-xylose; Permeabilization; Triton X-100; Xylitol