title: Production, characteristics and applications of phytase from a rhizosphere isolated Enterobacter sp. ACSS.
abstract: Optimization of process parameters for phytase production by Enterobacter sp. ACSS led to a 4.6-fold improvement in submerged fermentation, which was enhanced further in fed-batch fermentation. The purified 62 kDa monomeric phytase was optimally active at pH 2.5 and 60 °C and retained activity over a wide range of temperature (40-80 °C) and pH (2.0-6.0) with a half-life of 11.3 min at 80 °C. The kinetic parameters K m, V max, K cat, and K cat/K m of the pure phytase were 0.21 mM, 131.58 nmol mg(-1) s(-1), 1.64 × 10(3) s(-1), and 7.81 × 10(6) M(-1) s(-1), respectively. The enzyme was fairly stable in the presence of pepsin under physiological conditions. It was stimulated by Ca(+2), Mg(+2) and Mn(+2), but inhibited by Zn(+2), Cu(+2), Fe(+2), Pb(+2), Ba(+2) and surfactants. The enzyme can be applied in dephytinizing animal feeds, and the baking industry.
authors.affiliation: Department of Biotechnology and Food Technology, Faculty of Applied Sciences, Durban University of Technology, Durban, 4000, South Africa.
authors.forename: Ashira
authors.initials: A
authors.lastname: Chanderman
authors.affiliation: Department of Biotechnology and Food Technology, Faculty of Applied Sciences, Durban University of Technology, Durban, 4000, South Africa.
authors.forename: Adarsh Kumar
authors.initials: AK
authors.lastname: Puri
authors.affiliation: Department of Biotechnology and Food Technology, Faculty of Applied Sciences, Durban University of Technology, Durban, 4000, South Africa.
authors.forename: Kugen
authors.initials: K
authors.lastname: Permaul
authors.affiliation: Department of Biotechnology and Food Technology, Faculty of Applied Sciences, Durban University of Technology, Durban, 4000, South Africa. singhs@dut.ac.za.
authors.forename: Suren
authors.initials: S
authors.lastname: Singh
mesh_terms: D010832:6-Phytase / Q000096:biosynthesis / Q000737:chemistry; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry; D004754:Enterobacter / Q000201:enzymology* / Q000302:isolation & purification; D004795:Enzyme Stability; D006358:Hot Temperature; D058441:Rhizosphere
chemical_list: D001426:Bacterial Proteins; D010832:6-Phytase
keywords: Enterobacter sp. ACSS; Fed-batch; Feed; Phytase; Thermo-acid-stable