title: Evaluation of tendon healing using fibroblast like synoviocytes in rabbits: A biomechanical study.
abstract: Tendon never restores the complete biological and mechanical properties after healing. Several techniques are available for tissue-engineered biological augmentation for tendon healing like stem cells. Recently, synovium has been investigated as a source of cells for tissue engineering. In the present study, we investigated potentials of fibroblast like synoviocytes (FLSs) in tendon healing. Sixteen rabbits were divided randomly into control and treatment groups. One rabbit was used as a donor of synovial membrane (synovium). The injury model was unilateral complete transection through the middle one third of deep digital flexor tendon (DDFT). Subsequently, the tendon stumps were sutured with 3/0 nylon. In treatment group, 0.1 mL phosphate-buffered saline (PBS) solution containing 1 × 10(6) nucleated cells of FLSs was injected intratendinously at both tendon stumps just next to incision line. In control group, 0.1 mL PBS without FLSs was used for intratendinous injection. Model animals were euthanized at eight weeks, DDFTs were harvested and prepared for biomechanical study. Results of study showed that, there was no significant differences in biomechanical parameters values between FLSs treated and control groups. In conclusion, intratendinous injection of FLSs did not improve biomechanical properties during eight weeks in rabbit.
authors.affiliation: Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran;
authors.forename: Mahboobeh
authors.initials: M
authors.lastname: Azad-Tirgan
authors.affiliation: Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran;
authors.forename: Farshid
authors.initials: F
authors.lastname: Sarrafzadeh-Rezaei
authors.affiliation: Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran;
authors.forename: Hassan
authors.initials: H
authors.lastname: Malekinejad
authors.affiliation: Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran;
authors.forename: Rahim
authors.initials: R
authors.lastname: Hobbenaghi
authors.affiliation: Neurophysiology Research Center, Urmia University of Medical Sciences, Urmia, Iran.
authors.forename: Behnam
authors.initials: B
authors.lastname: Heshmatian
keywords: Biomechanical properties; Fibroblast like synoviocytes; Rabbit; Tendon