title: The Cyclic AMP Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells.
abstract: The cyclic AMP assay is a functional assay that is commonly used to determine the pharmacological behavior (agonists, antagonists, inverse agonists) of G-protein-coupled receptor (GPCR) ligands. Here, we describe the cyclic AMP assay that is carried out with commercially available non-radioligand ready-to-use kits and Chinese hamster ovarian (CHO) cells stably transfected with the human cannabinoid CB2 receptor.
authors.affiliation: School of Medicine, Medical Sciences & Nutrition, Institute of Medical Sciences, University of Aberdeen, AB252ZD, Foresterhill, Scotland, UK.
authors.forename: Pietro
authors.initials: P
authors.lastname: Marini
authors.affiliation: School of Medicine, Medical Sciences & Nutrition, Institute of Medical Sciences, University of Aberdeen, AB252ZD, Foresterhill, Scotland, UK. m.cascio@abdn.ac.uk.
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Cascio
authors.affiliation: School of Medicine, Medical Sciences & Nutrition, Institute of Medical Sciences, University of Aberdeen, AB252ZD, Foresterhill, Scotland, UK.
authors.forename: Roger G
authors.initials: RG
authors.lastname: Pertwee
mesh_terms: D000818:Animals; D001681:Biological Assay; D016466:CHO Cells; D002460:Cell Line; D003412:Cricetulus; D000242:Cyclic AMP / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D043885:Receptor, Cannabinoid, CB2 / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D043885:Receptor, Cannabinoid, CB2; D000242:Cyclic AMP
keywords: 3-Isobutyl-1-methylxanthine (IBMX); Agonist; Antagonist; Cannabinoid receptor type 2; Cyclic AMP; Forskolin (FSK); Inverse agonist