title: The SHIP2 interactor Myo1c is required for cell migration in 1321 N1 glioblastoma cells.
abstract: The phosphoinositide 5-phosphatases consist of several enzymes that have been shown to modulate cell migration and invasion. SHIP2, one family member, is known to interact with growth factor receptors and cytoskeletal proteins. In a human model of glioblastoma 1321 N1 cells, we recently identified Myo1c as a new interactor of SHIP2. This was shown in a complex of proteins also containing filamin A. We show here that SHIP2 localization at lamellipodia and ruffles is impaired in Myo1c depleted cells. In the absence of Myo1c, N1 cells tend to associate to form clusters. Cell migration is very much reduced in Myo1c depleted cells, concomitantly with a decrease in FAK Tyr397 phosphorylation, focal adhesion length and PI(4,5)P2 immunostaining. In N1 cells, Myo1c is thus important for lamellipodia formation to assemble a protein complex containing SHIP2 to facilitate cell migration.
authors.affiliation: IRIBHM, Université Libre de Bruxelles, Campus Erasme, Bâtiment C, 808 Route de Lennik, B-1070, Bruxelles, Belgium.
authors.forename: William's Elong
authors.initials: WE
authors.lastname: Edimo
authors.affiliation: IRIBHM, Université Libre de Bruxelles, Campus Erasme, Bâtiment C, 808 Route de Lennik, B-1070, Bruxelles, Belgium.
authors.forename: Ana Raquel
authors.initials: AR
authors.lastname: Ramos
authors.affiliation: IRIBHM, Université Libre de Bruxelles, Campus Erasme, Bâtiment C, 808 Route de Lennik, B-1070, Bruxelles, Belgium.
authors.forename: Somadri
authors.initials: S
authors.lastname: Ghosh
authors.affiliation: Neurophysiology Lab, Université Libre de Bruxelles, Campus Erasme, Bâtiment C, 808 Route de Lennik, B-1070, Bruxelles, Belgium.
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Vanderwinden
authors.affiliation: IRIBHM, Université Libre de Bruxelles, Campus Erasme, Bâtiment C, 808 Route de Lennik, B-1070, Bruxelles, Belgium. Electronic address: cerneux@ulb.ac.be.
authors.forename: Christophe
authors.initials: C
authors.lastname: Erneux
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D016764:Cell Polarity; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism; D005909:Glioblastoma / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D024461:Myosin Type I / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000378:metabolism; D000072183:Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatases / Q000378:metabolism*; D011554:Pseudopodia / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics
chemical_list: D019269:Phosphatidylinositol 4,5-Diphosphate; D034741:RNA, Small Interfering; D051416:Focal Adhesion Protein-Tyrosine Kinases; C098706:INPPL1 protein, human; D000072183:Phosphatidylinositol-3,4,5-Trisphosphate 5-Phosphatases; D024461:Myosin Type I; C460367:MYO1C protein, human
keywords: Migration; Myosin; Phosphoinositides; SHIP2