title: Canine Adipose-Derived Stem Cells: Purinergic Characterization and Neurogenic Potential for Therapeutic Applications.
abstract: The presented results evidence that canine adipose-derived stem cells (ADSCs) represent the premature population of stem cells with great biological potential and properties. ADCS are easy to obtain and culture, able to differentiate into the neurogenic lineage as well as it is easy to control their proliferation rate with nucleotides and nucleosides or analogues. We report that in vitro cultured canine ADSCs response to adenosine- and ATP-mediated stimulation. Differences in canine ADSCs and human mesenchymal stem cells in ecto-nucleotidase activity have been observed. The ecto-nucleotidase activity changes during ADSCs in vitro transdifferentiation into neurogenic lineage are fast and simple to analyze. Therefore, the simple analysis of ecto-enzymes activity allows for verification of the stem cells quality: their stemness or initiation of the differentiation process. The biological potential of the cells isolated from canine fat, as well as the good quality control of this cell culture, make them a promising tool for both experimental and therapeutic usage. J. Cell. Biochem. 118: 58-65, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Faculty of Biology and Environment Protection, Department of Biochemistry, Nicolaus Copernicus University, Torun, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Roszek
authors.affiliation: Faculty of Biology and Environment Protection, Department of Biochemistry, Nicolaus Copernicus University, Torun, Poland.
authors.forename: Noemi
authors.initials: N
authors.lastname: Makowska
authors.affiliation: Faculty of Biology and Environment Protection, Department of Biochemistry, Nicolaus Copernicus University, Torun, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Czarnecka
authors.affiliation: Faculty of Biology and Environment Protection, Department of Biochemistry, Nicolaus Copernicus University, Torun, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Porowińska
authors.affiliation: Faculty of Physics, Astronomy and Computer Science, Department of Biophysics and Medical Physics, Institute of Physics, Nicolaus Copernicus University, Torun, Poland.|Faculty of Biology and Environment Protection, Department of Biophysics, Nicolaus Copernicus University, Torun, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Dąbrowski
authors.affiliation: Max-Vet Veterinary Clinic, Torun, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Danielewska
authors.affiliation: Faculty of Physics, Astronomy and Computer Science, Department of Biophysics and Medical Physics, Institute of Physics, Nicolaus Copernicus University, Torun, Poland.
authors.forename: Wiesław
authors.initials: W
authors.lastname: Nowak
mesh_terms: D000241:Adenosine / Q000494:pharmacology*; D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D004285:Dogs; D006801:Humans; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism*; D013045:Species Specificity; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*
chemical_list: D000255:Adenosine Triphosphate; D000241:Adenosine
keywords: ADIPOSE-DERIVED STEM CELLS; ATOMIC FORCE MICROSCOPY; CANINE STEM CELLS; ECTO-NUCLEOTIDES; NEUROGENESIS; PURINERGIC SIGNALING