title: Effective and complex stimulation of the biodegradation system of fungus Cerrena unicolor by rapeseed meal fermentation.
abstract: The effect of supplementation of medium with rapeseed meal (RM) on production of biotechnologically important enzymes was investigated in submerged cultures of the white rot fungus Cerrena unicolor. The addition of RM (3.5% w/v) distinctly stimulated the activities of laccase, chitinase, and β-glucosidase. As compared to the control, the activities of chitinase, β-glucosidase, and laccase in the RM supplemented cultures were up to 4.1, 8.4, and 3.9 times higher, respectively. The results of the spectrophotometric and spectrofluorometric measurements were additionally confirmed by zymographic analysis of the samples. The level of sugars and phenolic compounds as well as the antioxidative ability of fungal preparations were also determined. The results obtained indicate that the submerged liquid fermentation of rapeseed meal can be proposed as an inexpensive and very effective method for biotechnological production of chitinase, β-glucosidase, and laccase by C. unicolor.
authors.affiliation: Department of Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Jaszek
authors.affiliation: Department of Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Miłek
authors.affiliation: Department of Biochemistry, Institute of Soil Science and Plant Cultivation, Puławy, Poland.
authors.forename: Jerzy
authors.initials: J
authors.lastname: Żuchowski
authors.affiliation: Department of Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland.
authors.forename: Dawid
authors.initials: D
authors.lastname: Stefaniuk
authors.affiliation: Chair and Department of Human Physiology, Medical University of Lublin, Lublin, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Prendecka
mesh_terms: D001673:Biodegradation, Environmental; D029741:Brassica rapa / Q000737:chemistry*; D002688:Chitinases / Q000096:biosynthesis; D003470:Culture Media; D005285:Fermentation; D005656:Fungal Proteins / Q000096:biosynthesis*; D042845:Laccase / Q000096:biosynthesis; D010636:Phenols / Q000378:metabolism; D011124:Polyporaceae / Q000201:enzymology*; D013481:Superoxides / Q000378:metabolism; D001617:beta-Glucosidase / Q000096:biosynthesis
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D010636:Phenols; D013481:Superoxides; D042845:Laccase; D002688:Chitinases; D001617:beta-Glucosidase