title: Development and validation of a liquid chromatography with tandem mass spectrometry method for the determination of isomangiferin in rat plasma with application to a preclinical pharmacokinetic study.
abstract: A sensitive and specific liquid chromatography with tandem mass spectrometry method was firstly developed for the measurement of isomangiferin in rat plasma. Chloramphenicol was selected as the internal standard. Sample preparation was carried out through a simple one-step protein precipitation procedure with methanol. Negative electrospray ionization was performed using multiple reaction monitoring mode with transitions of m/z 421.1/301.1 for isomangiferin, and 321.1/151.9 for chloramphenicol. The calibration curve was linear over the range of 0.1-600 ng/mL, with a lower limit of quantification at 0.1 ng/mL. The intra- and interday precisions (relative standard deviation) were no more than 8.2% and accuracies (relative error) were within the range of -8.4 to 2.2%. The recovery, matrix effect and stability under different conditions were all proved acceptable. The validated method has been successfully applied to a preclinical pharmacokinetic study of isomangiferin in rats for the first time.
authors.affiliation: Department of Pharmacy, Southwest Hospital, Third Military Medical University, Chongqing, P.R. China.
authors.forename: Fengjun
authors.initials: F
authors.lastname: Sun
authors.affiliation: Department of Pharmacy, Southwest Hospital, Third Military Medical University, Chongqing, P.R. China.
authors.forename: Zhirui
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Pharmacy, Southwest Hospital, Third Military Medical University, Chongqing, P.R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.affiliation: Department of Pharmacy, Southwest Hospital, Third Military Medical University, Chongqing, P.R. China.
authors.forename: Peiyuan
authors.initials: P
authors.lastname: Xia
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D008297:Male; D008968:Molecular Conformation; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry; D044004:Xanthones / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics*
chemical_list: D044004:Xanthones; C061492:isomangiferin
keywords: Isomangiferin; Liquid chromatography with tandem mass spectrometry; Pharmacokinetics; Rat plasma