title: A one-pot synthesis of α-l-threofuranosyl nucleoside triphosphates (tNTPs).
abstract: TNA (α-l-threofuranosyl nucleoside) triphosphates of adenosine (tATP), guanosine (tGTP), cytidine (tCTP), and thymidine (tTTP) were synthesized from their corresponding 3'-O-phosphoramidite derivatives using a novel one-pot reaction that is less moisture sensitive than traditional methods. The chemically synthesized tNTPs, despite containing an unnatural 3'-triphosphate moiety, are similar in thermal stability to natural nucleotide triphosphates.
authors.affiliation: Department of Pharmaceutical Sciences, University of California, Irvine, CA 92697-3958, USA.
authors.forename: Sujay P
authors.initials: SP
authors.lastname: Sau
authors.affiliation: Department of Pharmaceutical Sciences, University of California, Irvine, CA 92697-3958, USA.
authors.forename: John C
authors.initials: JC
authors.lastname: Chaput
mesh_terms: D015394:Molecular Structure; D009705:Nucleosides / Q000138:chemical synthesis* / Q000737:chemistry; D009711:Nucleotides
chemical_list: D009705:Nucleosides; C000621329:alpha-L-threofuranosyl nucleoside triphosphate; D009711:Nucleotides
keywords: Moisture tolerant; One-pot synthesis; Phosphoramidite; TNA triphosphate; Thermal stability