title: Using Bicistronic Baculovirus Expression Vector System to Screen the Compounds That Interfere with the Infection of Chikungunya Virus.
abstract: Chikungunya virus (CHIKV) is the etiologic agent of Chikungunya fever and has emerged in many countries over the past decade. There are no effective drugs for controlling the disease. A bicistronic baculovirus expression system was utilized to co-express CHIKV structural proteins C (capsid), E2 and E1 and the enhanced green fluorescence protein (EGFP) in Spodoptera frugiperda insect cells (Sf21). The EGFP-positive Sf21 cells fused with each other and with uninfected cells to form a syncytium is mediated by the CHIKV E1 allowing it to identify chemicals that can prevent syncytium formation. The compounds characterized by this method could be anti-CHIKV drugs.
authors.affiliation: Institute of Prevention Medicine, National Defense Medical Center, Taipei, Taiwan.
authors.forename: Szu-Cheng
authors.initials: SC
authors.lastname: Kuo
authors.affiliation: Bioengineering Group, Institute of Biologics, Development Center for Biotechnology, New Taipei, Taiwan.
authors.forename: Chao-Yi
authors.initials: CY
authors.lastname: Teng
authors.affiliation: Institute of Prevention Medicine, National Defense Medical Center, Taipei, Taiwan.
authors.forename: Yi-Jung
authors.initials: YJ
authors.lastname: Ho
authors.affiliation: Bioengineering Group, Institute of Biologics, Development Center for Biotechnology, New Taipei, Taiwan.
authors.forename: Ying-Ju
authors.initials: YJ
authors.lastname: Chen
authors.affiliation: Department of Bioscience Technology, Chung Yuan Christian University, Chung-Li, Taiwan. tywu@cycu.edu.tw.
authors.forename: Tzong-Yuan
authors.initials: TY
authors.lastname: Wu
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D016367:Baculoviridae / Q000235:genetics* / Q000378:metabolism; D036022:Capsid Proteins / Q000235:genetics* / Q000378:metabolism; D002459:Cell Fusion; D002646:Chikungunya virus / Q000187:drug effects* / Q000235:genetics; D004353:Drug Evaluation, Preclinical; D005822:Genetic Vectors / Q000235:genetics; D015726:Giant Cells / Q000187:drug effects; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D000067556:Internal Ribosome Entry Sites / Q000187:drug effects; D061987:Sf9 Cells; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D000998:Antiviral Agents; D036022:Capsid Proteins; D000067556:Internal Ribosome Entry Sites; D014759:Viral Envelope Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins
keywords: Baculovirus; Chikungunya virus; Internal ribosome entry site; Syncytium