title: N-glycan containing a core α1,3-fucose residue is required for basipetal auxin transport and gravitropic response in rice (Oryza sativa).
abstract: In plants, α1,3-fucosyltransferase (FucT) catalyzes the transfer of fucose from GDP-fucose to asparagine-linked GlcNAc of the N-glycan core in the medial Golgi. To explore the physiological significance of this processing, we isolated two Oryza sativa (rice) mutants (fuct-1 and fuct-2) with loss of FucT function. Biochemical analyses of the N-glycan structure confirmed that α1,3-fucose is missing from the N-glycans of allelic fuct-1 and fuct-2. Compared with the wild-type cv Kitaake, fuct-1 displayed a larger tiller angle, shorter internode and panicle lengths, and decreased grain filling as well as an increase in chalky grains with abnormal shape. The mutant allele fuct-2 gave rise to similar developmental abnormalities, although they were milder than those of fuct-1. Restoration of a normal tiller angle in fuct-1 by complementation demonstrated that the phenotype is caused by the loss of FucT function. Both fuct-1 and fuct-2 plants exhibited reduced gravitropic responses. Expression of the genes involved in tiller and leaf angle control was also affected in the mutants. We demonstrate that reduced basipetal auxin transport and low auxin accumulation at the base of the shoot in fuct-1 account for both the reduced gravitropic response and the increased tiller angle.
authors.affiliation: Division of Applied Life Science (BK21 +  program), PMBBRC, Gyeongsang National University, 501 Jinju-daero, Jinju, 52828, Korea.
authors.forename: Rikno
authors.initials: R
authors.lastname: Harmoko
authors.affiliation: Division of Applied Life Science (BK21 +  program), PMBBRC, Gyeongsang National University, 501 Jinju-daero, Jinju, 52828, Korea.
authors.forename: Jae Yong
authors.initials: JY
authors.lastname: Yoo
authors.affiliation: Division of Applied Life Science (BK21 +  program), PMBBRC, Gyeongsang National University, 501 Jinju-daero, Jinju, 52828, Korea.
authors.forename: Ki Seong
authors.initials: KS
authors.lastname: Ko
authors.affiliation: Division of Applied Life Science (BK21 +  program), PMBBRC, Gyeongsang National University, 501 Jinju-daero, Jinju, 52828, Korea.
authors.forename: Nirmal Kumar
authors.initials: NK
authors.lastname: Ramasamy
authors.affiliation: Division of Applied Life Science (BK21 +  program), PMBBRC, Gyeongsang National University, 501 Jinju-daero, Jinju, 52828, Korea.
authors.forename: Bo Young
authors.initials: BY
authors.lastname: Hwang
authors.affiliation: Division of Applied Life Science (BK21 +  program), PMBBRC, Gyeongsang National University, 501 Jinju-daero, Jinju, 52828, Korea.
authors.forename: Eun Ji
authors.initials: EJ
authors.lastname: Lee
authors.affiliation: Division of Applied Life Science (BK21 +  program), PMBBRC, Gyeongsang National University, 501 Jinju-daero, Jinju, 52828, Korea.
authors.forename: Ho Soo
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Integrative Omics Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Korea.
authors.forename: Kyung Jin
authors.initials: KJ
authors.lastname: Lee
authors.affiliation: Integrative Omics Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Korea.
authors.forename: Doo-Byoung
authors.initials: DB
authors.lastname: Oh
authors.affiliation: Crop Function Division, National Institute of Crop Science, Rural Development Administration, 181 Hyeoksin-ro, Wanju-gun, Jeollabuk-do, 55365, Korea.
authors.forename: Dool-Yi
authors.initials: DY
authors.lastname: Kim
authors.affiliation: Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN, 47907, USA.
authors.forename: Sanghun
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN, 47907, USA.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Division of Applied Life Science (BK21 +  program), PMBBRC, Gyeongsang National University, 501 Jinju-daero, Jinju, 52828, Korea.
authors.forename: Sang Yeol
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Division of Applied Life Science (BK21 +  program), PMBBRC, Gyeongsang National University, 501 Jinju-daero, Jinju, 52828, Korea.
authors.forename: Kyun Oh
authors.initials: KO
authors.lastname: Lee
mesh_terms: D000483:Alleles; D001692:Biological Transport; D004269:DNA, Bacterial / Q000235:genetics; D005643:Fucose / Q000737:chemistry / Q000378:metabolism*; D017343:Genes, Plant; D005816:Genetic Complementation Test; D018522:Gravitropism / Q000502:physiology*; D007210:Indoleacetic Acids / Q000378:metabolism*; D000073658:Loss of Function Mutation / Q000235:genetics; D020082:Magnaporthe / Q000502:physiology; D016254:Mutagenesis, Insertional / Q000235:genetics; D009154:Mutation / Q000235:genetics; D012275:Oryza / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology / Q000502:physiology*; D010641:Phenotype; D010935:Plant Diseases / Q000382:microbiology; D057865:Plant Immunity / Q000235:genetics; D010940:Plant Proteins / Q000378:metabolism; D018520:Plant Shoots / Q000502:physiology; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D012098:Reproduction; D012639:Seeds / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D007210:Indoleacetic Acids; D010940:Plant Proteins; D011134:Polysaccharides; C036483:T-DNA; D005643:Fucose
keywords: 3-fucosyltransferase; Oryza sativa; auxin transport; glycosylation; gravitropic response; plant development; α1