title: Generation of Equine-Induced Pluripotent Stem Cells and Analysis of Their Therapeutic Potential for Muscle Injuries.
abstract: Horse health has become a major concern with the expansion of horse-related industries and sports; the importance of healthy muscles for horse performance and daily activities is undisputed. Here we generated equine-induced pluripotent stem cells (E-iPSCs) by reprogramming equine adipose-derived stem cells (E-ADSCs) into iPSCs using a polycistronic lentiviral vector encoding four transcription factors (i.e., Oct4, Sox2, Klf4, and c-Myc) and then examined their pluripotent characteristics. Subsequently, established E-iPSCs were transplanted into muscle-injured Rag/ mdx mice. The histopathology results showed that E-iPSC-transplanted mice exhibited enhanced muscle regeneration compared to controls. In addition, E-iPSC-derived myofibers were observed in the injured muscles. In conclusion, we show that E-iPSCs could be successfully generated from equine ADSCs and transplanted into injured muscles and that E-iPSCs have the capacity to induce regeneration of injured muscles.
authors.affiliation: College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea.|Stem Cell Therapeutic Research Institute, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Eun-Mi
authors.initials: EM
authors.lastname: Lee
authors.affiliation: College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea.|Stem Cell Therapeutic Research Institute, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Ah-Young
authors.initials: AY
authors.lastname: Kim
authors.affiliation: College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea.|Stem Cell Therapeutic Research Institute, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Eun-Joo
authors.initials: EJ
authors.lastname: Lee
authors.affiliation: College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Jin-Kyu
authors.initials: JK
authors.lastname: Park
authors.affiliation: Cardiovascular Product Evaluation Center, College of Medicine, Yonsei University, Seoul, Republic of Korea.
authors.forename: Se-Il
authors.initials: SI
authors.lastname: Park
authors.affiliation: Department of Animal Biotechnology, Animal Resources Research Center, Incurable Disease Animal Model and Stem Cell Institute (IDASI), Konkuk University, Seoul, Republic of Korea.
authors.forename: Ssang-Goo
authors.initials: SG
authors.lastname: Cho
authors.affiliation: Department of Ophthalmology, School of Medicine, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Hong Kyun
authors.initials: HK
authors.lastname: Kim
authors.affiliation: Skeletal Diseases Genome Research Center, School of Medicine, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Shin-Yoon
authors.initials: SY
authors.lastname: Kim
authors.affiliation: College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea.|Stem Cell Therapeutic Research Institute, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Kyu-Shik
authors.initials: KS
authors.lastname: Jeong
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D004546:Elapid Venoms / Q000494:pharmacology; D058732:Embryoid Bodies / Q000378:metabolism / Q000473:pathology; D015870:Gene Expression / Q000187:drug effects; D006736:Horses; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism / Q000637:transplantation*; D000090062:Kruppel-Like Factor 4; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D008822:Mice, Transgenic; D018482:Muscle, Skeletal / Q000473:pathology / Q000502:physiology*; D009136:Muscular Dystrophies / Q000473:pathology / Q000628:therapy*; D018008:Myogenin / Q000235:genetics / Q000378:metabolism; D051763:PAX7 Transcription Factor / Q000235:genetics / Q000378:metabolism; D012038:Regeneration; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D004546:Elapid Venoms; C000715468:Klf4 protein, mouse; D000090062:Kruppel-Like Factor 4; D018008:Myogenin; D051763:PAX7 Transcription Factor; C497322:Pax7 protein, mouse; D014157:Transcription Factors; C005082:notexin
keywords: Horse; Induced pluripotent stem cells (iPSCs); Muscle regeneration; Transplantation