title: Electrochemical properties of seamless three-dimensional carbon nanotubes-grown graphene modified with horseradish peroxidase.
abstract: Horseradish peroxidase (HRP) was immobilized through sodium dodecyl sulfate (SDS) on the surface of a seamless three-dimensional hybrid of carbon nanotubes grown at the graphene surface (HRP-SDS/CNTs/G) and its electrochemical properties were investigated. Compared with graphene alone electrode modified with HRP via SDS (HRP-SDS/G electrode), the surface coverage of electroactive HRP at the CNTs/G electrode surface was approximately 2-fold greater because of CNTs grown at the graphene surface. Based on the increase in the surface coverage of electroactive HRP, the sensitivity to H2O2 at the HRP-SDS/CNTs/G electrode was higher than that at the HRP-SDS/G electrode. The kinetics of the direct electron transfer from the CNTs/G electrode to compound I and II of modified HRP was also analyzed.
authors.affiliation: Institute of Industrial Science, University of Tokyo, Komaba, Meguro-ku, Tokyo 153-8505, Japan; Department of Chemical and Environmental Engineering, University of California, Riverside, CA 92521, United States. Electronic address: kkomori@iis.u-tokyo.ac.jp.
authors.forename: Kikuo
authors.initials: K
authors.lastname: Komori
authors.affiliation: Department of Bioengineering, University of California, Riverside, CA 92521, United States.
authors.forename: Trupti
authors.initials: T
authors.lastname: Terse-Thakoor
authors.affiliation: Department of Chemical and Environmental Engineering, University of California, Riverside, CA 92521, United States. Electronic address: adani@engr.ucr.edu.
authors.forename: Ashok
authors.initials: A
authors.lastname: Mulchandani
mesh_terms: D055162:Biocatalysis; D015374:Biosensing Techniques; D004563:Electrochemistry; D004566:Electrodes; D004579:Electron Transport; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D006108:Graphite / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000737:chemistry* / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000032:analysis / Q000737:chemistry; D007700:Kinetics; D037742:Nanotubes, Carbon / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D006108:Graphite; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase
keywords: Biosensing; Carbon nanotubes; Graphene; Horseradish peroxidase; Hybrid