title: A Functional Assay for GPR55: Envision Protocol.
abstract: AlphaScreen(®) SureFire(®) assay is a novel technology that combines luminescent oxygen channeling technology, nano-beads, and monocloncal antibodies to detect the level of a selected protein in a volume lower than 5 μl. This method is more sensitive compared with the traditional enzyme-linked immunosorbent assays (ELISA), and can detect an increasing number of new targets. Here, we described a method for AlphaScreen(®) SureFire(®) assay that targets ERK1/2 phosphorylation, a primary downstream signaling pathway that conveys activation of GPR55 by L-α-lysophosphatidylinositol (LPI) and certain cannabinoids.
authors.affiliation: Department of Behavioral Sciences, Ariel University, Ariel, 40700, Israel. s.goffer@ariel.ac.il.|Institute of Medical Sciences, University of Aberdeen, Aberdeen, UK. s.goffer@ariel.ac.il.
authors.forename: Sharon
authors.initials: S
authors.lastname: Anavi-Goffer
authors.affiliation: Institute of Medical Sciences, University of Aberdeen, Aberdeen, UK.|Department of Pharmacology and Toxicology, Faculty of Medicine, University of Toronto, Toronto, ON, Canada.
authors.forename: Ruth A
authors.initials: RA
authors.lastname: Ross
mesh_terms: D001681:Biological Assay; D018929:Cell Culture Techniques; D057809:HEK293 Cells; D006801:Humans; D020935:MAP Kinase Signaling System; D010766:Phosphorylation; D043882:Receptors, Cannabinoid; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*
chemical_list: C524885:GPR55 protein, human; D043882:Receptors, Cannabinoid; D043562:Receptors, G-Protein-Coupled
keywords: AlphaLISA; AlphaScreen; Cannabinoid receptor; Enspire; GPR55; High-throughput screening; Phytocannabinoids