title: Use of recombinant porcine β-defensin 2 as a medicated feed additive for weaned piglets.
abstract: Post-weaning diarrhoea (PWD) in piglets is associated with colonization of the intestine with bacterial pathogens. In this study, we evaluated the use of recombinant porcine β-defensin 2 (rpBD2) as a medicated feed additive for weaned piglets. The crude extract from the culture supernatant of rpBD2-expressing Pichia pastoris was used as a medicated feed additive for weaned piglets. Dietary treatments included a positive control (basal diet + antibiotics, designated PC) and three different rpBD2 treatments without antibiotics (basal diet supplemented with 1, 5, or 15 g of crude rpBD2/kg basal diet, designated 1PD, 5PD, and 15PD, respectively). Of all the treatments, 5PD had the greatest impact on the weaned piglets. It increased their body weight, average daily weight gain, average daily feed intake, and intestinal villus height in the duodenum and jejunum, and reduced the incidence of PWD. The diversity of the cecal digesta and mucosa microflora was compared between the weaned piglets in the PC and 5PD groups. Piglets treated with 5PD had lower diversity indices and fewer bacterial pathogens in their cecal digesta and mucosa than the PC group. Our results demonstrate that crude rpBD2 could provide an alternative to the traditional antibiotic feed additives given to weaned piglets.
authors.affiliation: Microbiology Laboratory, China National Center for Food Safety Risk Assessment, No. 7 Panjiayuannanli Road, Chaoyang District, Beijing, 100021, China.|State Key Laboratory of Direct-Fed Microbial Engineering, No. B-3 Northern Territory of Zhongguancun Dongsheng Science and Technology Park, Haidian District, Beijing, 100193, China.
authors.forename: Zixin
authors.initials: Z
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Direct-Fed Microbial Engineering, No. B-3 Northern Territory of Zhongguancun Dongsheng Science and Technology Park, Haidian District, Beijing, 100193, China.
authors.forename: Anru
authors.initials: A
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Direct-Fed Microbial Engineering, No. B-3 Northern Territory of Zhongguancun Dongsheng Science and Technology Park, Haidian District, Beijing, 100193, China.
authors.forename: Linqi
authors.initials: L
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Direct-Fed Microbial Engineering, No. B-3 Northern Territory of Zhongguancun Dongsheng Science and Technology Park, Haidian District, Beijing, 100193, China.
authors.forename: Weiping
authors.initials: W
authors.lastname: Song
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No. 15 Fengtaidongdajie Street, Fengtai District, Beijing, 100071, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No. 15 Fengtaidongdajie Street, Fengtai District, Beijing, 100071, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Yin
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No. 15 Fengtaidongdajie Street, Fengtai District, Beijing, 100071, China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Zhou
authors.affiliation: Microbiology Laboratory, China National Center for Food Safety Risk Assessment, No. 7 Panjiayuannanli Road, Chaoyang District, Beijing, 100021, China.
authors.forename: Fengqin
authors.initials: F
authors.lastname: Li
mesh_terms: D000821:Animal Feed; D000818:Animals; D000890:Anti-Infective Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification; D001835:Body Weight / Q000187:drug effects; D002432:Cecum / Q000382:microbiology; D003967:Diarrhea / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D004353:Drug Evaluation, Preclinical; D004435:Eating; D005503:Food Additives / Q000008:administration & dosage / Q000627:therapeutic use*; D000069196:Gastrointestinal Microbiome / Q000187:drug effects; D007421:Intestine, Small / Q000187:drug effects / Q000648:ultrastructure; D008871:Microvilli / Q000648:ultrastructure; D010843:Pichia; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000627:therapeutic use; D021521:Ribotyping; D016415:Sequence Alignment; D013211:Staphylococcus aureus / Q000187:drug effects; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology / Q000517:prevention & control*; D014886:Weaning; D023083:beta-Defensins / Q000008:administration & dosage / Q000235:genetics / Q000627:therapeutic use*
chemical_list: D000890:Anti-Infective Agents; D005503:Food Additives; D011994:Recombinant Proteins; D023083:beta-Defensins