title: The molecular mechanism of G2M cell cycle arrest induced by AFB1 in the jejunum.
abstract: Aflatoxin B1 (AFB1) has potent hepatotoxic, carcinogenic, genotoxic, immunotoxic and other adverse effects in human and animals. The aim of this study was to investigate the molecular mechanism of G2/M cell cycle arrest induced by AFB1 in the jejunum of broilers. Broilers, as experimental animals, were fed 0.6 mg/kg AFB1 diet for 3 weeks. Our results showed that AFB1 reduced the jejunal villus height, villus height/crypt ratio and caused G2/M cell cycle arrest. The G2/M cell cycle was accompanied by the increase of ataxia telangiectasia mutated (ATM), p53, Chk2, p21 protein and mRNA expression, and the decrease of Mdm2, cdc25C, cdc2, cyclin B and proliferating cell nuclear antigen protein and mRNA expression. In conclusion, AFB1 blocked G2/M cell cycle by ATM pathway in the jejunum of broilers.
authors.affiliation: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Chengdu, Sichuan, PR China.
authors.forename: Heng
authors.initials: H
authors.lastname: Yin
authors.affiliation: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Chengdu, Sichuan, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Chengdu, Sichuan, PR China.|College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, PR China.
authors.forename: Xi
authors.initials: X
authors.lastname: Peng
authors.affiliation: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Chengdu, Sichuan, PR China.|College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, PR China.
authors.forename: Hengmin
authors.initials: H
authors.lastname: Cui
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: He
authors.affiliation: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Chengdu, Sichuan, PR China.|College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, PR China.
authors.forename: Zhicai
authors.initials: Z
authors.lastname: Zuo
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Ouyang
authors.affiliation: College of Biological and Agro-Forestry Engineering, Tongren University, Tongren, Guizhou, PR China.
authors.forename: Junde
authors.initials: J
authors.lastname: Fan
authors.affiliation: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Chengdu, Sichuan, PR China.|College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Fang
mesh_terms: D016604:Aflatoxin B1 / Q000633:toxicity*; D000818:Animals; D064007:Ataxia Telangiectasia Mutated Proteins / Q000378:metabolism*; D002455:Cell Division; D064447:Checkpoint Kinase 2 / Q000378:metabolism; D002645:Chickens; D056744:Cyclin B1 / Q000378:metabolism; D005434:Flow Cytometry; D059565:G2 Phase Cell Cycle Checkpoints / Q000187:drug effects*; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D007583:Jejunum / Q000187:drug effects / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology*; D008871:Microvilli / Q000187:drug effects; D011042:Poisons / Q000633:toxicity*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D020687:cdc25 Phosphatases / Q000378:metabolism
chemical_list: D056744:Cyclin B1; D011042:Poisons; D018809:Proliferating Cell Nuclear Antigen; D016159:Tumor Suppressor Protein p53; D016604:Aflatoxin B1; D064447:Checkpoint Kinase 2; D064007:Ataxia Telangiectasia Mutated Proteins; D020687:cdc25 Phosphatases
keywords: AFB1; G2/M phase; Pathology Section; cell cycle arrest; jejunum; mechanism