title: Clearing and Labeling Techniques for Large-Scale Biological Tissues.
abstract: Clearing and labeling techniques for large-scale biological tissues enable simultaneous extraction of molecular and structural information with minimal disassembly of the sample, facilitating the integration of molecular, cellular and systems biology across different scales. Recent years have witnessed an explosive increase in the number of such methods and their applications, reflecting heightened interest in organ-wide clearing and labeling across many fields of biology and medicine. In this review, we provide an overview and comparison of existing clearing and labeling techniques and discuss challenges and opportunities in the investigations of large-scale biological systems.
authors.affiliation: Department of Chemistry, Seoul National University, Seoul 08826, Korea.
authors.forename: Jinyoung
authors.initials: J
authors.lastname: Seo
authors.affiliation: Department of Biophysics and Chemical Biology, Seoul National University, Seoul 08826, Korea.
authors.forename: Minjin
authors.initials: M
authors.lastname: Choe
authors.affiliation: Department of Chemistry, Seoul National University, Seoul 08826, Korea.|Department of Biophysics and Chemical Biology, Seoul National University, Seoul 08826, Korea.|School of Biological Sciences, Seoul National University, Seoul 08826, Korea.|Institute of Molecular Biology and Genetics, Seoul National University, Seoul 08826, Korea.
authors.forename: Sung-Yon
authors.initials: SY
authors.lastname: Kim
mesh_terms: D021621:Imaging, Three-Dimensional / Q000379:methods; D018613:Microscopy, Confocal / Q000379:methods; D061848:Optical Imaging / Q000379:methods*; D013194:Staining and Labeling; D049490:Systems Biology / Q000379:methods*
keywords: 3D volume imaging; CLARITY; SWITCH; large-scale tissue clearing; stochastic electrotransport; whole-mount labeling