title: Multisite electrophysiological recordings by self-assembled loose-patch-like junctions between cultured hippocampal neurons and mushroom-shaped microelectrodes.
abstract: Substrate integrated planar microelectrode arrays is the "gold standard" method for millisecond-resolution, long-term, large-scale, cell-noninvasive electrophysiological recordings from mammalian neuronal networks. Nevertheless, these devices suffer from drawbacks that are solved by spike-detecting, spike-sorting and signal-averaging techniques which rely on estimated parameters that require user supervision to correct errors, merge clusters and remove outliers. Here we show that primary rat hippocampal neurons grown on micrometer sized gold mushroom-shaped microelectrodes (gMμE) functionalized simply by poly-ethylene-imine/laminin undergo self-assembly processes to form loose patch-like hybrid structures. More than 90% of the hybrids formed in this way record monophasic positive action potentials (APs). Of these, 34.5% record APs with amplitudes above 300 μV and up to 5,085 μV. This self-assembled neuron-gMμE configuration improves the recording quality as compared to planar MEA. This study characterizes and analyzes the electrophysiological signaling repertoire generated by the neurons-gMμE configuration, and discusses prospects to further improve the technology.
authors.affiliation: The Alexander Silberman Institute of Life Science, Department of Neurobiology, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Jerusalem, 91904, Israel.|The Harvey M. Kruger Family Center for Nanoscience, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Jerusalem, 91904, Israel.|The C. Smith Family and Prof. J. Elkes Laboratory for Collaborative Research in Psychobiology, Department of Neurobiology, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Jerusalem, 91904, Israel.
authors.forename: Nava
authors.initials: N
authors.lastname: Shmoel
authors.affiliation: The Alexander Silberman Institute of Life Science, Department of Neurobiology, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Jerusalem, 91904, Israel.|The C. Smith Family and Prof. J. Elkes Laboratory for Collaborative Research in Psychobiology, Department of Neurobiology, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Jerusalem, 91904, Israel.
authors.forename: Noha
authors.initials: N
authors.lastname: Rabieh
authors.affiliation: The Alexander Silberman Institute of Life Science, Department of Neurobiology, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Jerusalem, 91904, Israel.|The C. Smith Family and Prof. J. Elkes Laboratory for Collaborative Research in Psychobiology, Department of Neurobiology, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Jerusalem, 91904, Israel.
authors.forename: Silviya M
authors.initials: SM
authors.lastname: Ojovan
authors.affiliation: The Alexander Silberman Institute of Life Science, Department of Neurobiology, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Jerusalem, 91904, Israel.|The C. Smith Family and Prof. J. Elkes Laboratory for Collaborative Research in Psychobiology, Department of Neurobiology, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Jerusalem, 91904, Israel.
authors.forename: Hadas
authors.initials: H
authors.lastname: Erez
authors.affiliation: The Alexander Silberman Institute of Life Science, Department of Neurobiology, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Jerusalem, 91904, Israel.
authors.forename: Eilon
authors.initials: E
authors.lastname: Maydan
authors.affiliation: The Alexander Silberman Institute of Life Science, Department of Neurobiology, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Jerusalem, 91904, Israel.|The Harvey M. Kruger Family Center for Nanoscience, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Jerusalem, 91904, Israel.|The C. Smith Family and Prof. J. Elkes Laboratory for Collaborative Research in Psychobiology, Department of Neurobiology, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Jerusalem, 91904, Israel.
authors.forename: Micha E
authors.initials: ME
authors.lastname: Spira
mesh_terms: D000200:Action Potentials / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D004594:Electrophysiology / Q000295:instrumentation* / Q000379:methods; D004622:Embryo, Mammalian; D004867:Equipment Design; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D007797:Laminin / Q000737:chemistry; D008839:Microelectrodes; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D011094:Polyethyleneimine / Q000737:chemistry; D061251:Primary Cell Culture; D011724:Pyridazines / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D007797:Laminin; D011724:Pyridazines; D011094:Polyethyleneimine; C049853:gabazine