title: Regenerative Potential of Mesenchymal Stromal Cells: Age-Related Changes.
abstract: Preclinical and clinical studies have shown that a therapeutic effect results from mesenchymal stromal cells (MSCs) transplant. No systematic information is currently available regarding whether donor age modifies MSC regenerative potential on cutaneous wound healing. Here, we evaluate whether donor age influences this potential. Two different doses of bone marrow MSCs (BM-MSCs) from young, adult, or old mouse donors or two doses of their acellular derivatives mesenchymal stromal cells (acd-MSCs) were intradermally injected around wounds in the midline of C57BL/6 mice. Every two days, wound healing was macroscopically assessed (wound closure) and microscopically assessed (reepithelialization, dermal-epidermal junction, skin appendage regeneration, granulation tissue, leukocyte infiltration, and density dermal collagen fibers) after 12 days from MSC transplant. Significant differences in the wound closure kinetic, quality, and healing of skin regenerated were observed in lesions which received BM-MSCs from different ages or their acd-MSCs compared to lesions which received vehicle. Nevertheless, our data shows that adult's BM-MSCs or their acd-MSCs were the most efficient for recovery of most parameters analyzed. Our data suggest that MSC efficacy was negatively affected by donor age, where the treatment with adult's BM-MSCs or their acd-MSCs in cutaneous wound promotes a better tissue repair/regeneration. This is due to their paracrine factors secretion.
authors.affiliation: Center for Regenerative Medicine, School of Medicine, Clínica Alemana, Universidad del Desarrollo, Lo Barnechea, 7710162 Santiago, Chile.
authors.forename: Flavia
authors.initials: F
authors.lastname: Bruna
authors.affiliation: Center for Regenerative Medicine, School of Medicine, Clínica Alemana, Universidad del Desarrollo, Lo Barnechea, 7710162 Santiago, Chile.
authors.forename: David
authors.initials: D
authors.lastname: Contador
authors.affiliation: Center for Regenerative Medicine, School of Medicine, Clínica Alemana, Universidad del Desarrollo, Lo Barnechea, 7710162 Santiago, Chile.
authors.forename: Paulette
authors.initials: P
authors.lastname: Conget
authors.affiliation: Center for Regenerative Medicine, School of Medicine, Clínica Alemana, Universidad del Desarrollo, Lo Barnechea, 7710162 Santiago, Chile.
authors.forename: Benjamín
authors.initials: B
authors.lastname: Erranz
authors.affiliation: Production Unity of Advanced Therapy, Fundación Oftalmológica de Santander, Clínica Carlos Ardila Lulle (Foscal Internacional), Universidad Autónoma de Bucaramanga (UNAB), 681004153 Bucaramanga, Colombia.
authors.forename: Claudia L
authors.initials: CL
authors.lastname: Sossa
authors.affiliation: Production Unity of Advanced Therapy, Fundación Oftalmológica de Santander, Clínica Carlos Ardila Lulle (Foscal Internacional), Universidad Autónoma de Bucaramanga (UNAB), 681004153 Bucaramanga, Colombia.
authors.forename: Martha L
authors.initials: ML
authors.lastname: Arango-Rodríguez