title: Soluble antigen derived from IV larva of Angiostrongylus cantonensis promotes chitinase-like protein 3 (Chil3) expression induced by interleukin-13.
abstract: Angiostrongyliasis caused by Angiostrongylus cantonensis (A. cantonensis) is an emerging food-borne parasitic disease, which refers basically to eosinophilic meningitis. Chitinase-like protein 3 (Chil3), a member of chitinase-like protein family which has chemotactic activity for eosinophils, is reported to be highly upregulated in brain of mouse infected with A. cantonensis. The mechanisms of high expression of Chil3 and the association between A. cantonensis and Chil3 are rarely reported. In order to understand the mechanism of high expression of Chil3 in A. cantonensis-infected mouse, we measured the level of Chil3 in RAW 264.7 and BV2 cell lines stimulated with soluble antigen of A. cantonensis by qPCR and ELISA. To explore the role of Chil3 in inflammation caused by A. cantonensis, we extracted and cultured brain mononuclear cells (BMNCs) and detected the eosinophil chemotactic activity of Chil3 using transwell assay and flow cytometer. Furthermore, we treated the infected mice by injection with rmChil3 and then counted the number of larvae in brains of infected mice and treated mice to examine the association between the worm and Chil3. Our results showed the soluble antigen from A. cantonensis could promote the Chil3 expression in macrophage and microglial cell lines induced by interleukin-13. In conclusion, we supposed that high expression of Chil3 enhanced by soluble antigens from A. cantonensis might be the reason of serious eosinophil infiltration in mouse brain after A. cantonensis infection.
authors.affiliation: Key Laboratory for Tropical Diseases Control, Sun Yat-sen University, Guangzhou, 510080, China.|Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Wu
authors.affiliation: Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China.|Department of Clinical Laboratory, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510260, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wei
authors.affiliation: Key Laboratory for Tropical Diseases Control, Sun Yat-sen University, Guangzhou, 510080, China.|Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Key Laboratory for Tropical Diseases Control, Sun Yat-sen University, Guangzhou, 510080, China.|Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zeng
authors.affiliation: Key Laboratory for Tropical Diseases Control, Sun Yat-sen University, Guangzhou, 510080, China.|Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China.
authors.forename: Zilong
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Key Laboratory for Tropical Diseases Control, Sun Yat-sen University, Guangzhou, 510080, China.|Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China.
authors.forename: Zhiyue
authors.initials: Z
authors.lastname: Lv
authors.affiliation: Key Laboratory for Tropical Diseases Control, Sun Yat-sen University, Guangzhou, 510080, China. sunxi2@mail.sysu.edu.cn.|Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China. sunxi2@mail.sysu.edu.cn.
authors.forename: Xi
authors.initials: X
authors.lastname: Sun
authors.affiliation: Key Laboratory for Tropical Diseases Control, Sun Yat-sen University, Guangzhou, 510080, China. wuzhd@mail.sysu.edu.cn.|Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China. wuzhd@mail.sysu.edu.cn.|South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Sun Yat-sen University, Guangzhou, 510080, China. wuzhd@mail.sysu.edu.cn.
authors.forename: Zhongdao
authors.initials: Z
authors.lastname: Wu
mesh_terms: D017185:Angiostrongylus cantonensis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics / Q000378:metabolism*; D001921:Brain / Q000201:enzymology / Q000469:parasitology; D002688:Chitinases / Q000235:genetics* / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D018793:Interleukin-13 / Q000235:genetics / Q000378:metabolism*; D007814:Larva / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D012908:Snails; D017206:Strongylida Infections / Q000201:enzymology / Q000235:genetics / Q000378:metabolism* / Q000469:parasitology*
chemical_list: D000947:Antigens, Helminth; D018793:Interleukin-13; D002688:Chitinases
keywords: Angiostrongylus cantonensis; Antigen; Chil3; Eosinophil; Interleukin-13