title: The role of endogenous opioid peptides in the antinociceptive effect of 15-deoxy(Δ12,14)-prostaglandin J2 in the temporomandibular joint.
abstract: We have previously demonstrated that peripheral administration of 15d-PGJ2 in the Temporomandibular joint (TMJ) of rats can prevent nociceptor sensitization, mediated by peroxisome proliferator activated receptor-γ (PPAR-γ), and κ- and δ- opioid receptors. However, the mechanism that underlies the signaling of PPAR-γ (upon activation by 15d-PGJ2) to induce antinociception, and how the opioid receptors are activated via 15d-PGJ2 are not fully understood. This study demonstrates that peripheral antinociceptive effect of 15d-PGJ2 is mediated by PPAR-γ expressed in the inflammatory cells of TMJ tissues. Once activated by 15d-PGJ2, PPAR-γ induces the release of β-endorphin and dynorphin, which activates κ- and δ-opioid receptors in primary sensory neurons to induce the antinociceptive effect.
authors.affiliation: Department of Physiological Sciences, Laboratory of Orofacial Pain, Piracicaba Dental School, University of Campinas - UNICAMP, Av. Limeira, 901, Piracicaba, SP 13414-903, Brazil.
authors.forename: C G
authors.initials: CG
authors.lastname: Macedo
authors.affiliation: Laboratory of Immunology and Molecular Biology, São Leopoldo Mandic Institute and Research Center, Rua José Rocha Junqueira, 13 - Campinas, SP 13045-755, Brazil.
authors.forename: M H
authors.initials: MH
authors.lastname: Napimoga
authors.affiliation: Department of Physiological Sciences, Laboratory of Orofacial Pain, Piracicaba Dental School, University of Campinas - UNICAMP, Av. Limeira, 901, Piracicaba, SP 13414-903, Brazil.
authors.forename: L M
authors.initials: LM
authors.lastname: Rocha-Neto
authors.affiliation: Department of Physiological Sciences, Laboratory of Orofacial Pain, Piracicaba Dental School, University of Campinas - UNICAMP, Av. Limeira, 901, Piracicaba, SP 13414-903, Brazil.
authors.forename: H B
authors.initials: HB
authors.lastname: Abdalla
authors.affiliation: Department of Physiological Sciences, Laboratory of Orofacial Pain, Piracicaba Dental School, University of Campinas - UNICAMP, Av. Limeira, 901, Piracicaba, SP 13414-903, Brazil. Electronic address: juliana.napimoga@fop.unicamp.br.
authors.forename: J T
authors.initials: JT
authors.lastname: Clemente-Napimoga
mesh_terms: D000700:Analgesics / Q000008:administration & dosage* / Q000494:pharmacology; D000818:Animals; D004399:Dynorphins / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D018847:Opioid Peptides / Q000378:metabolism*; D047495:PPAR gamma / Q000378:metabolism; D015230:Prostaglandin D2 / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000187:drug effects; D013704:Temporomandibular Joint / Q000187:drug effects / Q000378:metabolism*; D001615:beta-Endorphin / Q000378:metabolism
chemical_list: C477819:15-deoxyprostaglandin J2; D000700:Analgesics; D018847:Opioid Peptides; D047495:PPAR gamma; D001615:beta-Endorphin; D004399:Dynorphins; D015230:Prostaglandin D2