title: Microsomal membrane proteome of low grade diffuse astrocytomas: Differentially expressed proteins and candidate surveillance biomarkers.
abstract: Diffuse astrocytoma (DA; WHO grade II) is a low-grade, primary brain neoplasm with high potential of recurrence as higher grade malignant form. We have analyzed differentially expressed membrane proteins from these tumors, using high-resolution mass spectrometry. A total of 2803 proteins were identified, 340 of them differentially expressed with minimum of 2 fold change and based on ≥2 unique peptides. Bioinformatics analysis of this dataset also revealed important molecular networks and pathways relevant to tumorigenesis, mTOR signaling pathway being a major pathway identified. Comparison of 340 differentially expressed proteins with the transcript data from Grade II diffuse astrocytomas reported earlier, revealed about 190 of the proteins correlate in their trends in expression. Considering progressive and recurrent nature of these tumors, we have mapped the differentially expressed proteins for their secretory potential, integrated the resulting list with similar list of proteins from anaplastic astrocytoma (WHO Grade III) tumors and provide a panel of proteins along with their proteotypic peptides, as a resource that would be useful for investigation as circulatory plasma markers for post-treatment surveillance of DA patients.
authors.affiliation: Centre for Cellular and Molecular Biology (CSIR), Hyderabad, India.
authors.forename: Ravindra Varma
authors.initials: RV
authors.lastname: Polisetty
authors.affiliation: Centre for Cellular and Molecular Biology (CSIR), Hyderabad, India.
authors.forename: Poonam
authors.initials: P
authors.lastname: Gautam
authors.affiliation: Centre for Cellular and Molecular Biology (CSIR), Hyderabad, India.|Institute of Bioinformatics, Bangalore, India.|Manipal University, Madhav Nagar, Manipal, India.
authors.forename: Manoj Kumar
authors.initials: MK
authors.lastname: Gupta
authors.affiliation: Institute of Bioinformatics, Bangalore, India.
authors.forename: Rakesh
authors.initials: R
authors.lastname: Sharma
authors.affiliation: Institute of Bioinformatics, Bangalore, India.
authors.forename: Harsha
authors.initials: H
authors.lastname: Gowda
authors.affiliation: Strand Life Sciences, Bangalore, India.
authors.forename: Durairaj
authors.initials: D
authors.lastname: Renu
authors.affiliation: Neuro-Oncology, Mazumdar Shaw Center for Translational Research, Narayana Health, Bangalore, India.
authors.forename: Bhadravathi Marigowda
authors.initials: BM
authors.lastname: Shivakumar
authors.affiliation: Mazumdar Shaw Medical Center, Narayana Health, Bangalore, India.
authors.forename: Akhila
authors.initials: A
authors.lastname: Lakshmikantha
authors.affiliation: Mazumdar Shaw Medical Center, Narayana Health, Bangalore, India.
authors.forename: Kiran
authors.initials: K
authors.lastname: Mariswamappa
authors.affiliation: Nizam's Institute of Medical Sciences (NIMS), Hyderabad, India.
authors.forename: Praveen
authors.initials: P
authors.lastname: Ankathi
authors.affiliation: Nizam's Institute of Medical Sciences (NIMS), Hyderabad, India.
authors.forename: Aniruddh K
authors.initials: AK
authors.lastname: Purohit
authors.affiliation: Nizam's Institute of Medical Sciences (NIMS), Hyderabad, India.
authors.forename: Megha S
authors.initials: MS
authors.lastname: Uppin
authors.affiliation: Nizam's Institute of Medical Sciences (NIMS), Hyderabad, India.
authors.forename: Challa
authors.initials: C
authors.lastname: Sundaram
authors.affiliation: Centre for Cellular and Molecular Biology (CSIR), Hyderabad, India.|Institute of Bioinformatics, Bangalore, India.|Neuro-Oncology, Mazumdar Shaw Center for Translational Research, Narayana Health, Bangalore, India.
authors.forename: Ravi
authors.initials: R
authors.lastname: Sirdeshmukh
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D001254:Astrocytoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D063646:Carcinogenesis / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D016022:Case-Control Studies; D019295:Computational Biology; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007425:Intracellular Membranes / Q000737:chemistry / Q000473:pathology; D008297:Male; D008861:Microsomes / Q000737:chemistry / Q000473:pathology; D058977:Molecular Sequence Annotation; D060787:Neoplasm Grading; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D009364:Neoplasm Recurrence, Local / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D020543:Proteome / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D058570:TOR Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D020543:Proteome; C546842:MTOR protein, human; D058570:TOR Serine-Threonine Kinases