title: The Effect of Combined Pulsed Wave Low-Level Laser Therapy and Human Bone Marrow Mesenchymal Stem Cell-Conditioned Medium on Open Skin Wound Healing in Diabetic Rats.
abstract: OBJECTIVE
  The nobility of this scientific study was to investigate the combined effects of pulsed wave low-level laser therapy (PWLLLT) and human bone marrow mesenchymal stem cell-conditioned medium (hBM-MSC-CM) on the biomechanical parameters of wounds in an experimental model for diabetes mellitus (DM).
  
  
  BACKGROUND
  PWLLLT exhibited biostimulatory effects on wounds in diabetic animals. Secretomes can be administered into wounds by the use of BM-MSC-CM.
  
  
  METHODS
  Type I DM was induced in rats by streptozotocin (STZ). Two wounds were made on proximal and distal parts in the dorsal region of each rat. Rats were divided into four groups. The first group was considered as the control group. The second group received hBM-MSC-CM. The third group received PWLLLT. The fourth group received hBM-MSC-CM+LASER. hBM-MSC-CM was administrated twice intraperitoneally. The proximal wounds in the third and fourth groups were treated with a pulsed laser by 890 nm wavelength, 80 Hz frequency, and 0.2 J/cm(2) energy densities. On the 15th day, a standard sample from each healing wound was submitted for biomechanical examination. The data were analyzed by analysis of variance test.
  
  
  RESULTS
  PWLLLT and hBM-MSC-CM, alone or in combination, significantly increased biomechanical parameters within the healing wounds. However, PWLLLT was statistically more effective compared with the hBM-MSC-CM. In the third and fourth groups, the numbers of wound closures were significantly enhanced in proximal part, contrary to the control ones.
  
  
  CONCLUSIONS
  It was magnificently attained that PWLLLT significantly accelerated the wound healing process in the experimental model for STZ-induced type I DM rats.
authors.affiliation: 1 School of Medicine, Shahid Beheshti University of Medical Sciences , Tehran, Iran .
authors.forename: Ramin
authors.initials: R
authors.lastname: Pouriran
authors.affiliation: 2 Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences , Tehran, Iran .|3 Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences , Tehran, Iran .
authors.forename: Abbas
authors.initials: A
authors.lastname: Piryaei
authors.affiliation: 2 Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences , Tehran, Iran .
authors.forename: Ataroalsadat
authors.initials: A
authors.lastname: Mostafavinia
authors.affiliation: 1 School of Medicine, Shahid Beheshti University of Medical Sciences , Tehran, Iran .
authors.forename: Sara
authors.initials: S
authors.lastname: Zandpazandi
authors.affiliation: 2 Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences , Tehran, Iran .
authors.forename: Farzane
authors.initials: F
authors.lastname: Hendudari
authors.affiliation: 2 Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences , Tehran, Iran .
authors.forename: Abdollah
authors.initials: A
authors.lastname: Amini
authors.affiliation: 2 Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences , Tehran, Iran .
authors.forename: Mohammad
authors.initials: M
authors.lastname: Bayat
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D017077:Culture Media, Conditioned; D003921:Diabetes Mellitus, Experimental; D004195:Disease Models, Animal; D005434:Flow Cytometry; D006801:Humans; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000293:injuries*; D014945:Wound Healing / Q000528:radiation effects*
chemical_list: D017077:Culture Media, Conditioned