title: Quercetin-3-O-β-D-Glucuronide Suppresses Lipopolysaccharide-Induced JNK and ERK Phosphorylation in LPS-Challenged RAW264.7 Cells.
abstract: Quercetin, a flavonol, has been reported to exhibit a wide range of biological properties including anti-oxidant and anti-inflammatory activities. However, pharmacological properties of quercetin-3-O-β-D-glucuronide (QG), a glycoside derivative of quercetin, have not been extensively examined. The objective of this study is to elucidate the anti-inflammatory property and underlying mechanism of QG in lipopolysaccharide (LPS)-challenged RAW264.7 macrophage cells in comparison with quercetin. QG significantly suppressed LPS-induced extracellular secretion of pro-inflammatory mediators such as nitric oxide (NO) and PGE₂, and pro-inflammatory protein expressions of iNOS and COX-2. To elucidate the underlying mechanism of the anti-inflammatory property of QG, involvement of MAPK signaling pathways was examined. QG significantly attenuated LPS-induced activation of JNK and ERK in concentration-dependent manners with a negligible effect on p38. In conclusion, the present study demonstrates QG exerts anti-inflammatory activity through the suppression of JNK and ERK signaling pathways in LPS-challenged RAW264.7 macrophage cells.
authors.affiliation: Department of Pharmacology, College of Medicine, Kangwon National University, Chuncheon 24341, Republic of Korea.
authors.forename: Jin-Young
authors.initials: JY
authors.lastname: Park
authors.affiliation: Department of Pharmacology, College of Medicine, Kangwon National University, Chuncheon 24341, Republic of Korea.
authors.forename: Man-Sup
authors.initials: MS
authors.lastname: Lim
authors.affiliation: Department of Pharmacology, College of Medicine, Kangwon National University, Chuncheon 24341, Republic of Korea.
authors.forename: Song-In
authors.initials: SI
authors.lastname: Kim
authors.affiliation: Department of Pharmacology, College of Medicine, Kangwon National University, Chuncheon 24341, Republic of Korea.
authors.forename: Hee Jae
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Department of Pharmacology, College of Medicine, Kangwon National University, Chuncheon 24341, Republic of Korea.
authors.forename: Sung-Soo
authors.initials: SS
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Kangwon National University, Chuncheon 24341, Republic of Korea.
authors.forename: Yong-Soo
authors.initials: YS
authors.lastname: Kwon
authors.affiliation: Department of Pharmacology, College of Medicine, Kangwon National University, Chuncheon 24341, Republic of Korea.
authors.forename: Wanjoo
authors.initials: W
authors.lastname: Chun
keywords: ERK; JNK; Lipopolysaccharide; Quercetin; Quercetin-3-O-β-D-glucuronide; RAW264.7 cells