title: The Displacement Binding Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells.
abstract: Displacement binding assays are nonfunctional assays mostly used with the aim of determining whether a certain compound (plant-derived or synthetic) is able to bind to a specific receptor with high affinity. Here, we describe the displacement binding assay that is carried out with a radioligand and CHO (Chinese Hamster Ovarian) cells stably transfected with the human cannabinoid CB2 receptor.
authors.affiliation: School of Medicine, Medical Sciences & Nutrition, Institute of Medical Sciences, University of Aberdeen, Foresterhill, AB252ZD, Scotland, UK. m.cascio@abdn.ac.uk.
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Cascio
authors.affiliation: School of Medicine, Medical Sciences & Nutrition, Institute of Medical Sciences, University of Aberdeen, Foresterhill, AB252ZD, Scotland, UK. p.marini@abdn.ac.uk.
authors.forename: Pietro
authors.initials: P
authors.lastname: Marini
authors.affiliation: School of Medicine, Medical Sciences & Nutrition, Institute of Medical Sciences, University of Aberdeen, Foresterhill, AB252ZD, Scotland, UK.
authors.forename: Roger G
authors.initials: RG
authors.lastname: Pertwee
mesh_terms: D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D003412:Cricetulus; D006801:Humans; D008024:Ligands; D011485:Protein Binding; D011869:Radioligand Assay / Q000379:methods*; D043885:Receptor, Cannabinoid, CB2 / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D008024:Ligands; D043885:Receptor, Cannabinoid, CB2
keywords: Affinity; Agonist; Antagonist; Binding; Cannabinoid CB2 receptors; Ligand; Radioligand