title: [Anti-proliferation Effect of Taraxacum mongolicum Extract in HepG2 Cells and Its Mechanism].
abstract: OBJECTIVE
  To study the anti-proliferation effect of Taraxacum mongolicum extract in HepG2 cells and its mechanism.
  
  
  METHODS
  The total proteins of HepG2 cells treated with Taraxacum mongolicum extract were. extracted and mitochondria-mediated apoptosis-related proteins (Survivin, Mcl-1, BCL-xL, BCL-2, Smac, BAX, Bad, Cytochrome c and Caspase-3/7/9) were detected by Western blot.
  
  
  RESULTS
  Taraxacum mongolicum extract obviously inhibited the proliferation of HepG2 cells and the expression of anti-apoptotic proteins (Survivin, BCL-xL and BCL-2), increased the expression of pro-apoptotic proteins (Smac and Caspase-3/7/9), and promoted the release of Cytochrome c from mitochondria to cytoplasm in HepG2 cells. The effects were in a dose-independent mode.
  
  
  CONCLUSIONS
  Taraxacum mongolicum extract can inhibit the proliferation of HepG2 cells and the anti-proliferation mechanism is related to mitochondria-mediated apoptosis.
authors.forename: Jun-bin
authors.initials: JB
authors.lastname: Guo
authors.forename: Hai-hong
authors.initials: HH
authors.lastname: Ye
authors.forename: Jian-feng
authors.initials: JF
authors.lastname: Chen
mesh_terms: D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D020169:Caspases / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D045304:Cytochromes c / Q000378:metabolism; D056945:Hep G2 Cells / Q000187:drug effects; D006801:Humans; D008928:Mitochondria / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology*; D031205:Taraxacum / Q000737:chemistry*
chemical_list: D051017:Apoptosis Regulatory Proteins; D010936:Plant Extracts; D045304:Cytochromes c; D020169:Caspases