title: The LysR-type transcriptional regulator, CidR, regulates stationary phase cell death in Staphylococcus aureus.
abstract: The Staphylococcus aureus LysR-type transcriptional regulator, CidR, activates the expression of two operons including cidABC and alsSD that display pro- and anti-death functions, respectively. Although several investigations have focused on the functions of different genes associated with these operons, the collective role of the CidR regulon in staphylococcal physiology is not clearly understood. Here we reveal that the primary role of this regulon is to limit acetate-dependent potentiation of cell death in staphylococcal populations. Although both CidB and CidC promote acetate generation and cell death, the CidR-dependent co-activation of CidA and AlsSD counters the effects of CidBC by redirecting intracellular carbon flux towards acetoin formation. From a mechanistic standpoint, we demonstrate that CidB is necessary for full activation of CidC, whereas CidA limits the abundance of CidC in the cell.
authors.affiliation: Center for Staphylococcal Research, Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska, 68198-5900, USA.
authors.forename: Sujata S
authors.initials: SS
authors.lastname: Chaudhari
authors.affiliation: Center for Staphylococcal Research, Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska, 68198-5900, USA.
authors.forename: Vinai C
authors.initials: VC
authors.lastname: Thomas
authors.affiliation: Center for Staphylococcal Research, Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska, 68198-5900, USA.
authors.forename: Marat R
authors.initials: MR
authors.lastname: Sadykov
authors.affiliation: Department of Microbiology, Molecular Genetics and Immunology, The University of Kansas Medical Center, MSN 3029, 3901 Rainbow Boulevard, Kansas City, KS, 66160, USA.
authors.forename: Jeffrey L
authors.initials: JL
authors.lastname: Bose
authors.affiliation: Center for Staphylococcal Research, Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska, 68198-5900, USA.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Ahn
authors.affiliation: Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, Nebraska, USA.
authors.forename: Matthew C
authors.initials: MC
authors.lastname: Zimmerman
authors.affiliation: Center for Staphylococcal Research, Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska, 68198-5900, USA. kbayles@unmc.edu.
authors.forename: Kenneth W
authors.initials: KW
authors.lastname: Bayles
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000378:metabolism; D009876:Operon; D050436:Regulatory Elements, Transcriptional; D018085:Regulon; D013211:Staphylococcus aureus / Q000166:cytology* / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D014157:Transcription Factors; C073470:LysR protein, Bacteria; D009238:N-Acetylmuramoyl-L-alanine Amidase