title: [The prevalence of Candida pelliculosa, Candida utilis, and Candida fabianii in the Olomouc University Hospital: epidemiological study].
abstract: BACKGROUND
  The aims of this study were to determine the prevalence of C. pelliculosa, C. utilis, and C. fabianii in clinical samples collected from patients hospitalized in the Olomouc University Hospital and compare their minimum inhibitory concentrations (MICs ) to nine systemic antifungals with respect to yeast species, patient age, gender, and site of infection.
  
  
  METHODS
  Identification was performed biochemically and using mass spectrometry (MALDI-TOF MS). MICs were determined by the broth dilution method.
  
  
  RESULTS
  Of a total of 163 clinical isolates, 119 were biochemically identified as C. pelliculosa and 44 as C. utilis. Using MALDI-TOF MS, 152 isolates were identified as C. fabianii, six as C. pelliculosa, three as C. utilis, and one as Ogataea polymorpha. The highest mean MICs were found in C. fabianii and in yeasts isolated from blood cultures and central venous catheters.
  
  
  CONCLUSIONS
  The MALDI-TOF MS found C. fabianii to be most prevalent in clinical samples as compared with the other studied species. The probable cause of discordant results between the two methods was the absence of C. fabianii in the database of the biochemical test kit which led to misidentification of this species. Higher MIC values in C. fabianii demonstrate the importance of the precise identification of this species.
authors.forename: L
authors.initials: L
authors.lastname: Svobodová
authors.forename: D
authors.initials: D
authors.lastname: Bednářová
authors.forename: P
authors.initials: P
authors.lastname: Hamal
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology; D002175:Candida / Q000187:drug effects / Q000302:isolation & purification*; D002177:Candidiasis / Q000453:epidemiology*; D016021:Epidemiologic Studies; D006785:Hospitals, University; D006801:Humans; D008826:Microbial Sensitivity Tests; D015995:Prevalence
chemical_list: D000935:Antifungal Agents
keywords: Candida fabianii - epidemiology - identification - MALDI-TOF - antifungals.