title: No evidence of exposure to environmental estrogens in two feral fish species sampled from the Yarra River, Australia: A comparison with Northern Hemisphere studies.
abstract: Environmental estrogens originate from a variety of sources including sewage treatment plant (STP) effluents and adverse physiological effects (endocrine disruption) have been observed in several fish species sampled downstream of STP discharges. In this study we examined common carp (Cyprinus carpio) and roach (Rutilis rutilis) for signs of exposure to environmental estrogens in the iconic Yarra River, Melbourne, Australia. The Yarra River flows through the city of Melbourne and more than 2 million people live within the catchment. Two STPs discharge water into the Yarra River within the middle reaches, and the areas immediately downstream of these discharge locations were the focus of this study. Carp and roach were chosen as test species since both have been utilised extensively for endocrine disruption research throughout Europe, North America and Asia, and data from various international studies was used for comparison with the results of the present study. Neither species showed evidence of exposure to environmental estrogens, with no elevation of plasma vitellogenin levels in males and no incidence of intersex gonads. Most physiological endpoints in both species from this study were within ranges reported in carp and roach from reference sites in other studies, however some degenerative histological changes in both male and female gonads were observed. Surface water samples showed no estrogenic activity (measured by the yeast-estrogen screen, YES), but did display strong anti-estrogenic and weak androgenic activity (measured by the yeast-androgen screen, YAS). Whilst the results show no evidence of impacts from environmental estrogens in the Yarra River, the presence of both anti-estrogenic and androgenic activity in water samples, as well as some gonadal changes in carp is concerning and indicates that our focus needs to broaden, in order to look for biological impacts in resident fauna that might be due to environmental pollutants other than environmental estrogens.
authors.affiliation: Centre for Aquatic Pollution Identification and Management (CAPIM), Bio 21 Institute, The University of Melbourne, Parkville, Victoria 3052, Australia. Electronic address: khassell@unimelb.edu.au.
authors.forename: Kathryn
authors.initials: K
authors.lastname: Hassell
authors.affiliation: Centre for Aquatic Pollution Identification and Management (CAPIM), Bio 21 Institute, The University of Melbourne, Parkville, Victoria 3052, Australia.
authors.forename: Vincent
authors.initials: V
authors.lastname: Pettigrove
authors.affiliation: Institute for Environment, Health and Societies, Brunel University, Uxbridge, Middlesex UB8 3PH, United Kingdom.
authors.forename: Nicola
authors.initials: N
authors.lastname: Beresford
authors.affiliation: Institute for Environment, Health and Societies, Brunel University, Uxbridge, Middlesex UB8 3PH, United Kingdom.
authors.forename: Susan
authors.initials: S
authors.lastname: Jobling
authors.affiliation: Environmental Contaminant Mitigation and Technologies Program, CSIRO Land and Water, Private Bag No. 2, Glen Osmond, SA 5064, Australia.
authors.forename: Anu
authors.initials: A
authors.lastname: Kumar
mesh_terms: D000818:Animals; D001208:Asia; D001315:Australia; D002347:Carps / Q000502:physiology; D003530:Cyprinidae / Q000502:physiology*; D012734:Disorders of Sex Development / Q000139:chemically induced / Q000662:veterinary; D052244:Endocrine Disruptors / Q000633:toxicity; D004785:Environmental Pollutants; D004958:Estradiol / Q000378:metabolism; D004967:Estrogens / Q000494:pharmacology / Q000633:toxicity*; D004970:Estrone / Q000494:pharmacology; D005060:Europe; D005260:Female; D006066:Gonads / Q000187:drug effects; D008297:Male; D009656:North America; D045483:Rivers / Q000737:chemistry; D012722:Sewage / Q000009:adverse effects; D013739:Testosterone / Q000031:analogs & derivatives / Q000378:metabolism; D014819:Vitellogenins / Q000097:blood; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
chemical_list: D052244:Endocrine Disruptors; D004785:Environmental Pollutants; D004967:Estrogens; D012722:Sewage; D014819:Vitellogenins; D014874:Water Pollutants, Chemical; D004970:Estrone; D013739:Testosterone; D004958:Estradiol; C003600:11-ketotestosterone
keywords: Biomarker; Carp; Endocrine disruption; Gonad; Roach; Vitellogenin