title: Site-specific characterization of N-linked glycosylation in human urinary glycoproteins and endogenous glycopeptides.
abstract: Glycosylation is a very important post-translational modification involved in various cellular processes, such as cell adhesion, signal transduction and immune response. Urine is a rich source of glycoproteins and attractive biological fluid for biomarker discovery, owing to its availability, ease of collection, and correlation with pathophysiology of diseases. Although the urinary proteomics have been explored previously, the urinary glycoproteome characterization remains challenging requiring the development and optimization of analytical and bioinformatics methods for protein glycoprofiling. This study describes the high confident identification of 472 unique N-glycosylation sites covering 256 urinary glycoproteins. Besides, 202 unique N-glycosylation sites were identified in low molecular weight endogenous glycopeptides, which belong to 90 glycoproteins. Global site-specific characterization of the N-linked glycan heterogeneity was achieved by intact glycopeptide analysis, revealing 303 unique glycopeptides most of them displaying complex/hybrid glycans composed by sialic acid and fucose. These datasets consist in a valuable resource of glycoproteins and N-glycosylation sites found in healthy human urine that can be further explored in different disorders, in which the N-linked glycosylation may be aberrant.
authors.affiliation: GlycoProteomics Laboratory, Departamento de Parasitologia, ICB, University of São Paulo, São Paulo, Brazil.
authors.forename: Rebeca
authors.initials: R
authors.lastname: Kawahara
authors.affiliation: GlycoProteomics Laboratory, Departamento de Parasitologia, ICB, University of São Paulo, São Paulo, Brazil.
authors.forename: Joyce
authors.initials: J
authors.lastname: Saad
authors.affiliation: GlycoProteomics Laboratory, Departamento de Parasitologia, ICB, University of São Paulo, São Paulo, Brazil.
authors.forename: Claudia Blanes
authors.initials: CB
authors.lastname: Angeli
authors.affiliation: GlycoProteomics Laboratory, Departamento de Parasitologia, ICB, University of São Paulo, São Paulo, Brazil. palmisano.gp@usp.br.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Palmisano
mesh_terms: D000328:Adult; D006020:Glycopeptides / Q000652:urine*; D006023:Glycoproteins / Q000652:urine*; D006031:Glycosylation; D006801:Humans; D008297:Male
chemical_list: D006020:Glycopeptides; D006023:Glycoproteins
keywords: Glycoforms; Glycopeptides; N-glycosylation; Urine