title: Calculibacillus koreensis gen. nov., sp. nov., an anaerobic Fe(III)-reducing bacterium isolated from sediment of mine tailings.
abstract: A strictly anaerobic bacterium, strain B5(T), was isolated from sediment of an abandoned coal mine in Taebaek, Republic of Korea. Cells of strain B5(T) were non-spore-forming, straight, Gram-positive rods. The optimum pH and temperature for growth were pH 7.0 and 30°C, respectively, while the strain was able to grow within pH and temperature ranges of 5.5-7.5 and 25-45°C, respectively. Growth of strain B5(T) was observed at NaCl concentrations of 0 to 6.0% (w/v) with an optimum at 3.0-4.0% (w/v). The polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, an unknown phospholipid and three unknown polar lipids. Strain B5(T) grew anaerobically by reducing nitrate, nitrite, ferric-citrate, ferric-nitrilotriacetate, elemental sulfur, thiosulfate, and anthraquinone-2-sulfonate in the presence of proteinaceous compounds, organic acids, and carbohydrates as electron donors. The isolate was not able to grow by fermentation. Strain B5(T) did not grow under aerobic or microaerobic conditions. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain B5(T) is most closely related to the genus Tepidibacillus (T. fermentans STGH(T); 96.3%) and Vulcanibacillus (V. modesticaldus BR(T); 94.6%). The genomic DNA G+C content (36.9 mol%) of strain B5(T) was higher than those of T. fermentans STGH(T) (34.8 mol%) and V. modesticaldus BR(T) (34.5 mol%). Based on its phenotypic, chemotaxonomic, and phylogenetic properties, we describe a new species of a novel genus Calculibacillus, represented by strain B5(T) (=KCTC 15397(T) =JCM 19989(T)), for which we propose the name Calculibacillus koreensis gen. nov., sp. nov.
authors.affiliation: Department of Microbiology, Chungbuk National University, Cheongju, 54896, Republic of Korea.
authors.forename: Ui-Gi
authors.initials: UG
authors.lastname: Min
authors.affiliation: Department of Microbiology, Chungbuk National University, Cheongju, 54896, Republic of Korea.
authors.forename: So-Jeong
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Department of Microbiology, Chungbuk National University, Cheongju, 54896, Republic of Korea.
authors.forename: Heeji
authors.initials: H
authors.lastname: Hong
authors.affiliation: Microbial Resources Center/KCTC, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, 56212, Republic of Korea.
authors.forename: Song-Gun
authors.initials: SG
authors.lastname: Kim
authors.affiliation: Department of Microbiology, Chungbuk National University, Cheongju, 54896, Republic of Korea.
authors.forename: Joo-Han
authors.initials: JH
authors.lastname: Gwak
authors.affiliation: Department of Microbiology, Chungbuk National University, Cheongju, 54896, Republic of Korea.
authors.forename: Man-Young
authors.initials: MY
authors.lastname: Jung
authors.affiliation: Department of Microbiology, Chungbuk National University, Cheongju, 54896, Republic of Korea.
authors.forename: Jong-Geol
authors.initials: JG
authors.lastname: Kim
authors.affiliation: Biomass and Waste Energy Laboratory, Korea Institute of Energy Research, Daejeon, 34129, Republic of Korea.
authors.forename: Jeong-Geol
authors.initials: JG
authors.lastname: Na
authors.affiliation: Department of Microbiology, Chungbuk National University, Cheongju, 54896, Republic of Korea. rhees@chungbuk.ac.kr.
authors.forename: Sung-Keun
authors.initials: SK
authors.lastname: Rhee
mesh_terms: D001406:Bacillaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D001421:Bacteria, Anaerobic / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D005290:Ferric Compounds / Q000378:metabolism*; D019015:Geologic Sediments / Q000382:microbiology*; D008906:Mining; D010084:Oxidation-Reduction; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D005290:Ferric Compounds; D012336:RNA, Ribosomal, 16S
keywords: Calculibacillus koreensis; coal mine tailings; iron