title: The protective effect of Nigella sativa against cisplatin-induced nephrotoxicity in rats.
abstract: OBJECTIVE
  The clinical use of cisplatin is highly restricted, because of its nephrotoxicity. In this study the protective effect of Nigella sativa (N. sativa) against cisplatin-induced nephrotoxicity was investigated in rats.
  
  
  METHODS
  In the current study, the effects of the administration of aqueous-ethanolic extract of N. sativa (100 and 200 mg/kg, BW) and vitamin E (100 mg/kg, BW) against blood and urine biochemical alterations and kidney function in rats treated with cisplatin were investigated. Cisplatin was injected at a dose of 6 mg/kg, BW, on the sixth day of the experiment.
  
  
  RESULTS
  The results indicated significant changes in serum urea and creatinine concentration, urine glucose concentration, and urine output in cisplatin group compared with control group. Serum urea and creatinine concentration in preventive and preventive+treatment vitamin E and preventive+treatment N. sativa (200 mg/kg, BW) groups and also serum creatinine concentration in preventive+treatment N. sativa (100 mg/kg, BW) group significantly decreased compared with cisplatin group. Urine glucose concentration in preventive and preventive+treatment N. sativa groups and urine output in preventive and preventive+treatment N. sativa (200 mg/kg, BW) groups significantly decreased compared with cisplatin group.Osmolarity excretion rate in preventive and preventive+treatment vitamin E and preventive N. sativa groups was significantly higher than control group.
  
  
  CONCLUSIONS
  The current study suggests that N. sativa extract and vitamin E in a dose- and time-dependent manner improved the serum and urine biochemical parameters and kidney function in cisplatin-induced nephrotoxicity in rats. However, it needs more investigations to determine the mechanism of N. sativa action on cisplatin-induced kidney toxicity.
authors.affiliation: Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Sara
authors.initials: S
authors.lastname: Hosseinian
authors.affiliation: Neurogenic Inflammation Research Center, Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Abolfazl
authors.initials: A
authors.lastname: Khajavi Rad
authors.affiliation: Neurocognitive Research Center, Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Mousa-Al-Reza
authors.initials: MA
authors.lastname: Hadjzadeh
authors.affiliation: Departmant of Pathology, Qaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Nema
authors.initials: N
authors.lastname: Mohamadian Roshan
authors.affiliation: Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Shahrzad
authors.initials: S
authors.lastname: Havakhah
authors.affiliation: Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Somayeh
authors.initials: S
authors.lastname: Shafiee
keywords: Cisplatin; Nigella sativa; Renal Failure; Vitamin E