title: PSF engineering in multifocus microscopy for increased depth volumetric imaging.
abstract: Imaging and localizing single molecules with high accuracy in a 3D volume is a challenging task. Here we combine multifocal microscopy, a recently developed volumetric imaging technique, with point spread function engineering to achieve an increased depth for single molecule imaging. Applications in 3D single molecule localization-based super-resolution imaging is shown over an axial depth of 4 µm as well as for the tracking of diffusing beads in a fluid environment over 8 µm.
authors.affiliation: Laboratoire Physico-Chimie, Institut Curie, PSL Research University, CNRS UMR168, 75005, Paris, France; Sorbonne Universités, UPMC Univ Paris 06, 75005, Paris, France; Transcription Imaging Consortium, Janelia Research Campus, 19700 Helix Drive, Ashburn VA, 20147, USA; bassam.hajj@curie.fr.
authors.forename: Bassam
authors.initials: B
authors.lastname: Hajj
authors.affiliation: Laboratoire Physico-Chimie, Institut Curie, PSL Research University, CNRS UMR168, 75005, Paris, France; Sorbonne Universités, UPMC Univ Paris 06, 75005, Paris, France; Transcription Imaging Consortium, Janelia Research Campus, 19700 Helix Drive, Ashburn VA, 20147, USA.
authors.forename: Mohamed
authors.initials: M
authors.lastname: El Beheiry
authors.affiliation: Laboratoire Physico-Chimie, Institut Curie, PSL Research University, CNRS UMR168, 75005, Paris, France; Sorbonne Universités, UPMC Univ Paris 06, 75005, Paris, France; Transcription Imaging Consortium, Janelia Research Campus, 19700 Helix Drive, Ashburn VA, 20147, USA; maxime.dahan@curie.fr.
authors.forename: Maxime
authors.initials: M
authors.lastname: Dahan
keywords: (100.6640) Superresolution; (100.6890) Three-dimensional image processing; (170.6900) Three-dimensional microscopy