title: Enantioselective separation of defined endocrine-disrupting nonylphenol isomers.
abstract: Nonylphenol is in the focus of worldwide endocrine-disrupter research and accounted for as a priority hazardous substance of the Water Framework Directive of the European Union. Technical nonylphenol consists of a very complex mixture of isomers and enantiomers. As estrogenic effect and degradation behavior in environmental processes of single nonylphenols are heavily dependent on the structure of the nonyl side chain, it is absolutely necessary to consider the nonylphenol problem from an isomer and enantiomer-specific viewpoint. In this study, an enantiomer-specific separation of eight defined synthesized nonylphenol isomers by five different special chiral cyclodextrin columns was performed underivatized and after methylation, silylation, and acylation. This work demonstrates that three columns out of the investigated five show an excellent separation behavior for the studied different nonylphenol isomers and can be used for the enantiomer-specific determination of nonylphenols in food, other biological matrices, and environmental samples in the future. Graphical abstract Enantiomeric pair of 4-NP170 (4-[1-ethyl-1,3,3-trimethylbutyl]phenol).
authors.affiliation: Institute of Nutrition and Food Sciences, Food Chemistry, University of Bonn, Endenicher Allee 11-13, 53115, Bonn, Germany.
authors.forename: Ismail-Hakki
authors.initials: IH
authors.lastname: Acir
authors.affiliation: Institute of Nutrition and Food Sciences, Bioanalytics, University of Bonn, Endenicher Allee 11-13, 53115, Bonn, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Wüst
authors.affiliation: Institute of Nutrition and Food Sciences, Food Chemistry, University of Bonn, Endenicher Allee 11-13, 53115, Bonn, Germany. k.guenther@uni-bonn.de.
authors.forename: Klaus
authors.initials: K
authors.lastname: Guenther
mesh_terms: D045424:Complex Mixtures / Q000737:chemistry / Q000302:isolation & purification*; D052244:Endocrine Disruptors / Q000737:chemistry* / Q000302:isolation & purification*; D005410:Flame Ionization / Q000379:methods*; D007536:Isomerism; D010636:Phenols / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D045424:Complex Mixtures; D052244:Endocrine Disruptors; D010636:Phenols; C025256:nonylphenol
keywords: Chiral analysis; Endocrine disruptors; Gas chromatography; Nonylphenol