title: An Immunohistochemical Survey of SNARE Proteins Shows Distinct Patterns of Expression in Hematolymphoid Neoplasia.
abstract: OBJECTIVE
  Five proteins from the soluble N-ethylmaleimide-sensitive factor activating protein receptor (SNARE) complex family were studied in normal hematopoietic cells in bone marrow; normal lymphocytes at different stages of maturation and differentiation in bone marrow, thymus, tonsil, and lymph node; malignant lymphomas; and leukemias.
  
  
  METHODS
  Sixty-eight reactive and 380 hematopoietic and lymphoid neoplasms were immunohistochemically stained for syntaxin 7 (STX7), vesicle-associated membrane proteins (VAMP2, VAMP7, VAMP8), and synaptosomal-associated protein 23 (SNAP23).
  
  
  RESULTS
  STX7 has potential for being a useful marker for distinguishing between normal B precursors (hematogones) vs B lymphoblasts, as well as between the "popcorn" cells of nodular lymphocyte-predominant Hodgkin lymphoma vs the Reed-Sternberg cells of classic Hodgkin lymphoma or the B cells of T-cell, histiocyte-rich B-cell lymphoma. VAMP2 is uniquely expressed by both reactive and malignant plasma cells, in contrast to B-cell non-Hodgkin lymphoma. There is differential expression of SNARE proteins in normal and neoplastic lymphoid tissue depending on lymphocyte maturation stage.
  
  
  CONCLUSIONS
  Differential SNARE protein expression in the lymphoid system may have potential use in diagnosis and may offer clues to lymphoma biology. VAMP2 is a promising new plasma cell marker.
authors.affiliation: From the Department of Pathology, Montefiore Medical Center/Albert Einstein College of Medicine, Bronx, NY rekhabhat@manipalhospitals.com.
authors.forename: Rekha
authors.initials: R
authors.lastname: Bhat
authors.affiliation: Department of Pathology, Hackensack University Medical Center, Hackensack, NJ.
authors.forename: Pritish K
authors.initials: PK
authors.lastname: Bhattacharyya
authors.affiliation: From the Department of Pathology, Montefiore Medical Center/Albert Einstein College of Medicine, Bronx, NY.
authors.forename: Howard
authors.initials: H
authors.lastname: Ratech
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D001853:Bone Marrow / Q000378:metabolism; D019337:Hematologic Neoplasms / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D008221:Lymphoid Tissue / Q000378:metabolism; D050600:SNARE Proteins / Q000032:analysis / Q000096:biosynthesis*; D046888:Tissue Array Analysis
chemical_list: D014408:Biomarkers, Tumor; D050600:SNARE Proteins
keywords: Leukemia; Lymphoma; SNAP23; SNARE; Syntaxin7; VAMP2; VAMP7; VAMP8