title: Talaromyces marneffei laccase modifies THP-1 macrophage responses.
abstract: Talaromyces (Penicillium) marneffei is an emerging opportunistic pathogen associated with HIV infection, particularly in Southeast Asia and southern China. The rapid uptake and killing of T. marneffei conidia by phagocytic cells along with the effective induction of an inflammatory response by the host is essential for disease control. T. marneffei produces a number of different laccases linked to fungal virulence. To understand the role of the various laccases in T. marneffei, laccase-encoding genes were investigated. Targeted single, double and triple gene deletions of laccases encoding lacA, lacB, and lacC showed no significant phenotypic effects suggesting redundancy of function. When a fourth laccase-encoding gene, pbrB, was deleted in the ΔlacA ΔlacB ΔlacC background, the quadruple mutant displayed delayed conidiation and the conidia were more sensitive to H2O2, sodium dodecyl sulfate (SDS), and antifungal agents than wild-type and other transformants. Conidia of the quadruple mutant showed marked differences in their interaction with the human monocyte cell line, THP-1 such that phagocytosis was significantly higher when compared with the wild-type at one and 2 hours of incubation while the phagocytic index was significantly different from 15 to 120 minutes. In addition, killing of the quadruple mutant by THP-1 cells was more efficient at 2 and 4 hours of incubation. The levels of the proinflammatory cytokines TNF-α, IL-1β and IL-6 from THP-1 cells infected with the quadruple mutant were also significantly increased in comparison with wild-type. The results demonstrate that production of laccases by T. marneffei actually promotes the pathogen's resistance to innate host defenses.
authors.affiliation: a Department of Microbiology , Faculty of Medicine, Chiang Mai University , Chiang Mai , Thailand.|b Faculty of Medical Technology, Nakhon Ratchasima College , Nakhon Ratchasima , Thailand.
authors.forename: Ariya
authors.initials: A
authors.lastname: Sapmak
authors.affiliation: a Department of Microbiology , Faculty of Medicine, Chiang Mai University , Chiang Mai , Thailand.
authors.forename: Jutikul
authors.initials: J
authors.lastname: Kaewmalakul
authors.affiliation: c Department of Medicine, Division of Infectious Diseases, and Department of Microbiology and Immunology , Albert Einstein College of Medicine , Bronx , NY , USA.
authors.forename: Joshua D
authors.initials: JD
authors.lastname: Nosanchuk
authors.affiliation: a Department of Microbiology , Faculty of Medicine, Chiang Mai University , Chiang Mai , Thailand.
authors.forename: Nongnuch
authors.initials: N
authors.lastname: Vanittanakom
authors.affiliation: d Genetics, Genomics and Development, School of BioSciences, The University of Melbourne , Victoria , Australia.
authors.forename: Alex
authors.initials: A
authors.lastname: Andrianopoulos
authors.affiliation: a Department of Microbiology , Faculty of Medicine, Chiang Mai University , Chiang Mai , Thailand.
authors.forename: Kritsada
authors.initials: K
authors.lastname: Pruksaphon
authors.affiliation: a Department of Microbiology , Faculty of Medicine, Chiang Mai University , Chiang Mai , Thailand.
authors.forename: Sirida
authors.initials: S
authors.lastname: Youngchim
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology; D002460:Cell Line; D017353:Gene Deletion; D005800:Genes, Fungal; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007113:Immunity, Innate; D053583:Interleukin-1beta / Q000276:immunology; D015850:Interleukin-6 / Q000276:immunology; D042845:Laccase / Q000235:genetics* / Q000378:metabolism*; D008264:Macrophages / Q000276:immunology* / Q000382:microbiology*; D009000:Monocytes / Q000382:microbiology; D010641:Phenotype; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013172:Spores, Fungal / Q000187:drug effects; D032901:Talaromyces / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity*; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D000935:Antifungal Agents; D053583:Interleukin-1beta; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D012967:Sodium Dodecyl Sulfate; D006861:Hydrogen Peroxide; D042845:Laccase
keywords: Penicillium marneffei; laccases; monocytes; proinflammatory cytokines; talaromyces marneffei