title: In vivo recombination efficiency of two site-specific recombination systems, VCre/VloxP and SCre/SloxP, in medaka (Oryzias latipes).
abstract: The present study delineates the in vivo efficiency of two site-specific recombination systems, VCre/VloxP and SCre/SloxP, in medaka (Oryzias latipes). VCre, SCre, and Cre RNA was microinjected into fertilized medaka eggs belonging to three transgenic lines harboring VloxP, SloxP, and loxP cassette. VCre induced site-specific recombination specifically at VloxP sequence and SCre at SloxP sequence without any cross-reactivity. These findings provide two novel alternative recombination systems in vivo in addition to the existing Cre/loxP and Flp/FRT systems, thus enabling sophisticated gene expression in model organisms.
authors.affiliation: Division of Applied Bioscience, Graduate School of Agriculture, Kyoto university, Sakyo-ku, 606-8502.
authors.forename: Kenta
authors.initials: K
authors.lastname: Kishimoto
authors.affiliation: Chromosome Engineering Team, Department of Technology Development, Kazusa DNA Research Institute, Kisarazu, 292-0818, Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Nakayama
authors.affiliation: Division of Applied Bioscience, Graduate School of Agriculture, Kyoto university, Sakyo-ku, 606-8502.
authors.forename: Masato
authors.initials: M
authors.lastname: Kinoshita
mesh_terms: D000818:Animals; D005260:Female; D005786:Gene Expression Regulation; D005818:Genetic Engineering / Q000379:methods*; D019426:Integrases; D008297:Male; D009990:Oryzias / Q000235:genetics / Q000378:metabolism; D011995:Recombination, Genetic
chemical_list: C045073:Cre recombinase; D019426:Integrases
keywords: Cre/loxP; SCre/SloxP; VCre/VloxP; medaka; site-specific recombination