title: Ethylene glycol assisted preparation of Ti(4+)-modified polydopamine coated magnetic particles with rough surface for capture of phosphorylated proteins.
abstract: The reversible protein phosphorylation is very important in regulating almost all aspects of cell life, while the enrichment of phosphorylated proteins still remains a technical challenge. In this work, polydopamine (PDA) modified magnetic particles with rough surface (rPDA@Fe3O4) were synthesized by introduction of ethylene glycol in aqueous solution. The PDA coating possessing a wealth of catechol hydroxyl groups could serve as an active medium to immobilize titanium ions through the metal-catechol chelation, which makes the fabrication of titanium ions modified rPDA@Fe3O4 particles (Ti(4+)-rPDA@Fe3O4) simple and very convenient. The spherical Ti(4+)-rPDA@Fe3O4 particles have a surface area of 37.7 m(2) g(-1) and superparamagnetism with a saturation magnetization value of 38.4 emu g(-1). The amount of Ti element in the particle was measured to be 3.93%. And the particles demonstrated good water dispersibility. The particles were used as adsorbents for capture of phosphorylated proteins and they demonstrated affinity and specificity for phosphorylated proteins due to the specific binding sites (Ti(4+)). Factors affecting the adsorption of phosphorylated proteins on Ti(4+)-rPDA@Fe3O4 particles were investigated. The adsorption capacity of Ti(4+)-rPDA@Fe3O4 particles for κ-casein was 1105.6 mg g(-1). Furthermore, the particles were successfully applied to isolate phosphorylated proteins in milk samples, which demonstrated that Ti(4+)-rPDA@Fe3O4 particles had potential application in selective separation of phosphorylated proteins.
authors.affiliation: Ministry of Education Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Ma
authors.affiliation: Ministry of Education Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Ding
authors.affiliation: Ministry of Education Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yao
authors.affiliation: Ministry of Education Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China. Electronic address: jiali@scnu.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Jia
mesh_terms: D000327:Adsorption; D000818:Animals; D002364:Caseins / Q000737:chemistry / Q000302:isolation & purification; D002417:Cattle; D019855:Ethylene Glycol / Q000737:chemistry*; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D007211:Indoles / Q000737:chemistry*; D060328:Magnetic Phenomena; D010750:Phosphoproteins / Q000737:chemistry* / Q000302:isolation & purification; D011108:Polymers / Q000737:chemistry*; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D002364:Caseins; D007211:Indoles; D010750:Phosphoproteins; D011108:Polymers; C568283:polydopamine; D014867:Water; D014025:Titanium; D019855:Ethylene Glycol; D052203:Ferrosoferric Oxide
keywords: Immobilized metal ion affinity chromatography; Phosphorylated proteins; Separation; Ti(4+)-rPDA@Fe(3)O(4) particles