title: [BIOLOGICAL ACTIVITY OF ANTIMICROBIAL PEPTIDES FROM CHICKENS THROMBOCYTES].
abstract: OBJECTIVE
  Isolation and study of biological activity of antimicrobial peptides from chickens thrombocytes.
  
  
  METHODS
  Peptides from chickens thrombocytes, obtained by reverse-phase high-performance liquid chromatography method with stepped and linear gradients of concentration increase of the organic solvent were used in the study. Their antimicrobial activity was determined by microtitration method in broth; mechanism of biological effect--by using fluorescent spectroscopy method with DNA-tropic dyes.
  
  
  RESULTS
  Individual fractions of peptides were isolated from chickens thrombocytes, that possess antimicrobial activity against Staphylococcus aureus P209 and Escherichia coli K12. A disruption of integrity of barrier structures of microorganisms under the effect of thrombocyte antimicrobial peptides and predominance of cells with damaged membrane in the population of E. coli was established.
  
  
  CONCLUSIONS
  The data obtained on antimicrobial activity and mechanism of bactericidal effect of the peptide fractions from chickens thrombocytes isolated for the first time expand the understanding of functional properties of chickens thrombocytes and open a perspective for their further study with the aim of use as antimicrobial means.
authors.forename: M V
authors.initials: MV
authors.lastname: Sycheva
authors.forename: A S
authors.initials: AS
authors.lastname: Vasilchenko
authors.forename: E A
authors.initials: EA
authors.lastname: Rogozhin
authors.forename: T M
authors.initials: TM
authors.lastname: Pashkova
authors.forename: L P
authors.initials: LP
authors.lastname: Popova
authors.forename: O L
authors.initials: OL
authors.lastname: Kartashova
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000494:pharmacology*; D001792:Blood Platelets / Q000737:chemistry*; D002462:Cell Membrane / Q000187:drug effects; D002645:Chickens; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D008826:Microbial Sensitivity Tests; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides