title: miR-145a-5p Promotes Myoblast Differentiation.
abstract: MicroRNAs are a class of 18-22-nucleotide noncoding RNAs that posttranscriptionally regulate gene expression and have been shown to play an important role during myoblast differentiation. In this study, we found that the expression of miR-145a-5p was gradually increased during C2C12 myoblast differentiation, and miR-145a-5p inhibitors or mimics significantly suppressed or promoted the relative expression of specific myogenesis related marker genes. Moreover, overexpression or inhibition of miR-145a-5p enhanced or repressed the expression of some special genes involved in the endogenous Wnt signaling pathway during C2C12 myoblast differentiation, including Wnt5a, LRP5, Axin2, and β-catenin. These results indicated that miR-145a-5p might be considered as a new myogenic differentiation-associated microRNA that can promote C2C12 myoblast differentiation by enhancing genes related to myoblasts differentiation.
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Du
authors.affiliation: Sichuan Province General Station of Animal Husbandry, Chengdu 611130, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Linyuan
authors.initials: L
authors.lastname: Shen
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada T6G 2P5.
authors.forename: Huaigang
authors.initials: H
authors.lastname: Lei
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Luo
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Yihui
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Peiwen
authors.initials: P
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Pu
authors.affiliation: Department of Animal Science, Xichang College, Xichang 61500, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Surong
authors.initials: S
authors.lastname: Shuai
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Xuewei
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Shunhua
authors.initials: S
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D002460:Cell Line; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D032446:Myoblasts / Q000166:cytology* / Q000378:metabolism; D060449:Wnt Signaling Pathway / Q000235:genetics
chemical_list: C543499:MIRN145a microRNA, mouse; D035683:MicroRNAs