title: Effects of positive end-expiratory pressure and 30% inspired oxygen on pulmonary mechanics and atelectasis in cats undergoing non-bronchoscopic bronchoalveolar lavage.
abstract: Objectives The objective of this study was to determine if modification of inspired oxygen concentration or positive end-expiratory pressure (PEEP) would alter bronchoalveolar lavage (BAL)-induced changes in pulmonary mechanics or atelectasis, as measured using ventilator-acquired pulmonary mechanics and thoracic CT. Methods Six experimentally asthmatic cats underwent anesthesia and non-bronchoscopic BAL, each under four randomized treatment conditions: 100% oxygen, zero PEEP; 30% oxygen, zero PEEP; 100% oxygen, PEEP 2 cmH2O; and 30% oxygen, PEEP 2 cmH2O. Pulse oximetry was used to estimate oxygen saturation (SpO2). Ventilator-acquired pulmonary mechanics and thoracic CT scans were collected prior to BAL and at 1, 5 and 15 mins post-BAL. Results While receiving 100% oxygen, no cat had SpO2<91%. Some cats receiving 30% oxygen had decreased saturation immediately post-BAL (mean ± SD 70.8 ± 31%), but 6/8 of these had SpO2>90% by 1 min later. There was a significant increase in airway resistance and a decrease in lung compliance following BAL, but there was no significant difference between treatment groups. Cats receiving no PEEP and 30% oxygen conserved better aeration of the lung parenchyma in BAL-sampled areas than those receiving no PEEP and 100% oxygen. Conclusions and relevance Alterations in pulmonary mechanics or atelectasis may not be reflected by SpO2following BAL. The use of 30% inspired oxygen concentration failed to show any significant improvement in pulmonary mechanics but did diminish atelectasis. In some cats, it was also associated with desaturation of hemoglobin. The use of PEEP in this study did not show any effect on our outcome parameters. Further studies using higher PEEP (5-10 cmH2O) and intermediate inspired oxygen concentration (40-60%) are warranted to determine if they would confer clinical benefit in cats undergoing diagnostic BAL.
authors.forename: Christa
authors.initials: C
authors.lastname: Bernhard
authors.affiliation: Current address: Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Montréal, Québec, Canada.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Masseau
authors.forename: John
authors.initials: J
authors.lastname: Dodam
authors.forename: Hilton
authors.initials: H
authors.lastname: Outi
authors.forename: Stacy
authors.initials: S
authors.lastname: Krumme
authors.forename: Kaitlin
authors.initials: K
authors.lastname: Bishop
authors.forename: Amber
authors.initials: A
authors.lastname: Graham
authors.forename: Carol
authors.initials: C
authors.lastname: Reinero
mesh_terms: D000818:Animals; D001249:Asthma / Q000175:diagnosis / Q000628:therapy / Q000662:veterinary*; D018893:Bronchoalveolar Lavage / Q000009:adverse effects / Q000662:veterinary; D002371:Cat Diseases / Q000175:diagnosis* / Q000000981:diagnostic imaging / Q000628:therapy; D002415:Cats; D005260:Female; D008297:Male; D010092:Oximetry / Q000662:veterinary; D010100:Oxygen / Q000008:administration & dosage*; D011175:Positive-Pressure Respiration / Q000009:adverse effects / Q000662:veterinary; D001261:Pulmonary Atelectasis / Q000209:etiology / Q000662:veterinary*; D014057:Tomography, X-Ray Computed / Q000662:veterinary
chemical_list: D010100:Oxygen