title: The antiviral activity of arctigenin in traditional Chinese medicine on porcine circovirus type 2.
abstract: Arctigenin (ACT) is a phenylpropanoid dibenzylbutyrolactone lignan extracted from the traditional herb Arctium lappa L. (Compositae) with anti-viral and anti-inflammatory effects. Here, we investigated the antiviral activity of ACT found in traditional Chinese medicine on porcine circovirus type 2 (PCV2) in vitro and in vivo. Results showed that dosing of 15.6-62.5μg/mL ACT could significantly inhibit the PCV2 proliferation in PK-15 cells (P<0.01). Dosing of 62.5μg/mL ACT 0, 4 or 8h after challenge inoculation significantly inhibited the proliferation of 1MOI and 10MOI in PK-15 cells (P<0.01), and the inhibitory effect of ACT dosing 4h or 8h post-inoculation was greater than 0h after dosing (P<0.01). In vivo test with mice challenge against PCV2 infection demonstrated that intraperitoneal injection of 200μg/kg ACT significantly inhibited PCV2 proliferation in the lungs, spleens and inguinal lymph nodes, with an effect similar to ribavirin, demonstrating the effectiveness of ACT as an antiviral agent against PCV2 in vitro and in vivo. This compound, therefore, may have the potential to serve as a drug for protection of pigs against the infection of PCV2.
authors.affiliation: Wuhan Institute of Animal Husbandry and Veterinary Science, Wuhan Academy of Agricultural Science & Technology, Wuhan 430208, Hubei, People's Republic of China; College of Veterinary Medicine, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, Hubei, People's Republic of China.
authors.forename: Wentao
authors.initials: W
authors.lastname: Li
authors.affiliation: Wuhan Institute of Animal Husbandry and Veterinary Science, Wuhan Academy of Agricultural Science & Technology, Wuhan 430208, Hubei, People's Republic of China.
authors.forename: Erguang
authors.initials: E
authors.lastname: Jin
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, Hubei, People's Republic of China.
authors.forename: Qigai
authors.initials: Q
authors.lastname: He
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, Hubei, People's Republic of China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Yan
authors.affiliation: College of Veterinary Medicine, China Agricultural University, Beijing, People's Republic of China.
authors.forename: Hanchun
authors.initials: H
authors.lastname: Yang
authors.affiliation: Wuhan Institute of Animal Husbandry and Veterinary Science, Wuhan Academy of Agricultural Science & Technology, Wuhan 430208, Hubei, People's Republic of China.
authors.forename: Shiyu
authors.initials: S
authors.lastname: Gong
authors.affiliation: Wuhan Institute of Animal Husbandry and Veterinary Science, Wuhan Academy of Agricultural Science & Technology, Wuhan 430208, Hubei, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Wuhan Institute of Animal Husbandry and Veterinary Science, Wuhan Academy of Agricultural Science & Technology, Wuhan 430208, Hubei, People's Republic of China.
authors.forename: Shulin
authors.initials: S
authors.lastname: Fu
authors.affiliation: Wuhan Institute of Animal Husbandry and Veterinary Science, Wuhan Academy of Agricultural Science & Technology, Wuhan 430208, Hubei, People's Republic of China.
authors.forename: Xiabing
authors.initials: X
authors.lastname: Chen
authors.affiliation: Wuhan Institute of Animal Husbandry and Veterinary Science, Wuhan Academy of Agricultural Science & Technology, Wuhan 430208, Hubei, People's Republic of China.
authors.forename: Shengqiang
authors.initials: S
authors.lastname: Ye
authors.affiliation: Wuhan Institute of Animal Husbandry and Veterinary Science, Wuhan Academy of Agricultural Science & Technology, Wuhan 430208, Hubei, People's Republic of China. Electronic address: qyg2016@163.com.
authors.forename: Yunguo
authors.initials: Y
authors.lastname: Qian
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D031170:Arctium / Q000737:chemistry; D018173:Circoviridae Infections / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000187:drug effects* / Q000502:physiology; D005260:Female; D005663:Furans / Q000494:pharmacology*; D007274:Injections, Intraperitoneal; D017705:Lignans / Q000494:pharmacology*; D008516:Medicine, Chinese Traditional; D051379:Mice; D008807:Mice, Inbred BALB C; D011897:Random Allocation; D013552:Swine; D013553:Swine Diseases / Q000517:prevention & control* / Q000821:virology
chemical_list: D000998:Antiviral Agents; D005663:Furans; D017705:Lignans; C071942:arctigenin
keywords: Antiviral activity; Arctigenin; Porcine circovirus type 2