title: Fat-water MRI of a diet-induced obesity mouse model at 15.2T.
abstract: Quantitative fat-water MRI (FWMRI) methods provide valuable information about the distribution, volume, and composition of adipose tissue (AT). Ultra high field FWMRI of animal models may have the potential to provide insights into the progression of obesity and its comorbidities. Here, we present quantitative FWMRI with all known confounder corrections on a 15.2T preclinical scanner for noninvasive in vivo monitoring of an established diet-induced obesity mouse model. Male C57BL/6J mice were placed on a low-fat (LFD) or a high-fat diet (HFD). Three-dimensional (3-D) multiple gradient echo MRI at 15.2T was performed at baseline, 4, 8, 12, and 16 weeks after diet onset. A 3-D fat-water separation algorithm and additional processing were used to generate proton-density fat fraction (PDFF), local magnetic field offset, and [Formula: see text] maps. We examined these parameters in perirenal AT ROIs from LFD and HFD mice. The data suggest that PDFF, local field offset, and [Formula: see text] have different time course behaviors between LFD and HFD mice over 16 weeks. This work suggests FWMRI at 15.2T may be a useful tool for longitudinal studies of adiposity due to the advantages of ultra high field although further investigation is needed to understand the observed time course behavior.
authors.affiliation: Vanderbilt University Institute of Imaging Science, Medical Center North, AA-1105, 1161 21st Avenue South, Nashville, Tennessee 37232-2310, United States; Vanderbilt University School of Medicine, Department of Radiology and Radiological Sciences, Medical Center North, 1161 21st Avenue South, Nashville, Tennessee 37232-2310, United States.
authors.forename: Henry H
authors.initials: HH
authors.lastname: Ong
authors.affiliation: Vanderbilt University School of Medicine , Department of Molecular Physiology and Biophysics, Light Hall, 2215 Garland Avenue, Nashville, Tennessee 37232-0615, United States.
authors.forename: Corey D
authors.initials: CD
authors.lastname: Webb
authors.affiliation: Vanderbilt University School of Medicine , Department of Molecular Physiology and Biophysics, Light Hall, 2215 Garland Avenue, Nashville, Tennessee 37232-0615, United States.
authors.forename: Marnie L
authors.initials: ML
authors.lastname: Gruen
authors.affiliation: Vanderbilt University School of Medicine , Department of Molecular Physiology and Biophysics, Light Hall, 2215 Garland Avenue, Nashville, Tennessee 37232-0615, United States.
authors.forename: Alyssa H
authors.initials: AH
authors.lastname: Hasty
authors.affiliation: Vanderbilt University Institute of Imaging Science, Medical Center North, AA-1105, 1161 21st Avenue South, Nashville, Tennessee 37232-2310, United States; Vanderbilt University School of Medicine, Department of Radiology and Radiological Sciences, Medical Center North, 1161 21st Avenue South, Nashville, Tennessee 37232-2310, United States; Vanderbilt University School of Medicine, Department of Molecular Physiology and Biophysics, Light Hall, 2215 Garland Avenue, Nashville, Tennessee 37232-0615, United States.
authors.forename: John C
authors.initials: JC
authors.lastname: Gore
authors.affiliation: Vanderbilt University Institute of Imaging Science, Medical Center North, AA-1105, 1161 21st Avenue South, Nashville, Tennessee 37232-2310, United States; Vanderbilt University School of Medicine, Department of Radiology and Radiological Sciences, Medical Center North, 1161 21st Avenue South, Nashville, Tennessee 37232-2310, United States.
authors.forename: E Brian
authors.initials: EB
authors.lastname: Welch
keywords: MRI; adipose tissue; fat quantification; inflammation; iron; mouse; obesity