title: Associations of the Novel Polymorphisms of Periostin and Platelet-Derived Growth Factor Receptor-Like Genes with Egg Production Traits in Local Chinese Dagu Hens.
abstract: The periostin (POSTN) and platelet-derived growth factor receptor-like (PDGFRL) genes are implicated in regulation of hen ovarian development. In the present study, these genes were explored as possible molecular markers associated with egg production, egg weight and body weight in Chinese Dagu hens. Samples were analyzed using the PCR-single strand conformation polymorphism (PCR-SSCP) method, followed by sequencing analysis, and three novel single nucleotide polymorphisms (SNPs) were identified within the candidate genes. Among them, an A/T transversion at base position 2727 in intron 2 of POSTN gene was found to be polymorphic and named SNP A2727T; and two transitions, G/A at position 6761 and A/G at base 6839 in exon 2 of PDGFRL gene were detected and named SNPs G6761A and A6839G, respectively. For the SNP A2727T, a total of 360 Dagu hens were classified as AA and AB genotypes, allele A was found present at a higher frequency. Moreover, the AA genotype was significantly correlated with higher hen-housed egg production (HHEP) at 43, 57, and 66 weeks (wks) of age and with a higher egg weight (EW) at 30 wks (P < 0.05). For the two linked SNPs (G6761A and A6839G) in the PDGFRL fragment, the hens were typed into TT, TC and CC genotypes, with the T allele shown to be dominant. The TT genotype was correlated with higher HHEP at 57 and 66 wks of age; genotype CC associated with the highest body weight and EW at 30 and 43 wks (P < 0.05), while it was correlated with the lowest HHEP at 57 and 66 wks of age (P < 0.05). Furthermore, five haplotypes were reconstructed based on these SNPs, with the AATT haplotype associated with the highest HHEP at 43 to 66 wks of age and higher EW at 30 wks (P < 0.05). Collectively, these SNPs identified in this study might be used as a potential molecular marker favorable to genetic improvement of egg productivity in chicken breeding.
authors.affiliation: a Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology , Jilin Agricultural University , Changchun , Jilin , China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Jing
authors.affiliation: a Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology , Jilin Agricultural University , Changchun , Jilin , China.
authors.forename: Xuesong
authors.initials: X
authors.lastname: Shan
authors.affiliation: a Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology , Jilin Agricultural University , Changchun , Jilin , China.
authors.forename: Fang
authors.initials: F
authors.lastname: Mu
authors.affiliation: a Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology , Jilin Agricultural University , Changchun , Jilin , China.
authors.forename: Ning
authors.initials: N
authors.lastname: Qin
authors.affiliation: a Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology , Jilin Agricultural University , Changchun , Jilin , China.
authors.forename: HongYan
authors.initials: H
authors.lastname: Zhu
authors.affiliation: a Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology , Jilin Agricultural University , Changchun , Jilin , China.
authors.forename: Dehui
authors.initials: D
authors.lastname: Liu
authors.affiliation: b Jilin Grain Group Agriculture and Livestock Co., Ltd. , Changchun , Jilin , China.
authors.forename: Shuguo
authors.initials: S
authors.lastname: Yuan
authors.affiliation: a Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology , Jilin Agricultural University , Changchun , Jilin , China.
authors.forename: Rifu
authors.initials: R
authors.lastname: Xu
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000235:genetics*; D001835:Body Weight / Q000235:genetics*; D001947:Breeding; D015815:Cell Adhesion Molecules / Q000235:genetics*; D002645:Chickens / Q000235:genetics*; D002681:China; D004531:Eggs; D005260:Female; D017479:Receptors, Platelet-Derived Growth Factor / Q000235:genetics*
chemical_list: D030161:Avian Proteins; D015815:Cell Adhesion Molecules; D017479:Receptors, Platelet-Derived Growth Factor
keywords: Chinese Dagu hens; PDGFRL; POSTN gene; egg production traits; gene polymorphisms