title: Expression of metallothioneins I and II related to oxidative stress in the liver of aluminium-treated rats.
abstract: Hepatotoxicity, induced by aluminium chloride (AlCl3), has been well studied but there are no reports about liver metallothionein (MT) genes induction. Therefore, it is of interest to establish the mechanism involving the relation between MT gene expression levels and the oxidative stress status in hepatic cells of aluminium-treated rats. Aluminium (Al) was administered to rats in their drinking water at a dose of 50 mg/kg body weight for three weeks. AlCl3provoked hepatotoxicity objectified by an increase in malondialdehyde (MDA), hydrogen peroxide (H2O2), advanced oxidation protein products (AOPP), protein carbonyls (PCO) and a decrease in reduced glutathione (GSH), non-protein thiols (NPSH) and vitamin C. CAT and Glutathione peroxidase (GPx) activities were decreased while Mn-SOD gene expression, total Metallothionein content and MT I and MT II genes induction were increased. There are changes in plasma of some trace elements, albumin levels, transaminases, LDH and ALP activities. All these changes were supported by histopathological observations.
authors.affiliation: a Animal Physiology Laboratory.
authors.forename: Imen
authors.initials: I
authors.lastname: Ghorbel
authors.affiliation: a Animal Physiology Laboratory.
authors.forename: Mariem
authors.initials: M
authors.lastname: Chaabane
authors.affiliation: a Animal Physiology Laboratory.
authors.forename: Awatef
authors.initials: A
authors.lastname: Elwej
authors.affiliation: b Anatomopathology Laboratory, CHU Habib Bourguiba , and.
authors.forename: Ons
authors.initials: O
authors.lastname: Boudawara
authors.affiliation: c Biochemistry Laboratory, CHU Hedi Chaker, University of Sfax , Sfax , Tunisia.
authors.forename: Sameh
authors.initials: S
authors.lastname: Abdelhedi
authors.affiliation: c Biochemistry Laboratory, CHU Hedi Chaker, University of Sfax , Sfax , Tunisia.
authors.forename: Kamel
authors.initials: K
authors.lastname: Jamoussi
authors.affiliation: b Anatomopathology Laboratory, CHU Habib Bourguiba , and.
authors.forename: Tahya
authors.initials: T
authors.lastname: Boudawara
authors.affiliation: a Animal Physiology Laboratory.
authors.forename: Najiba
authors.initials: N
authors.lastname: Zeghal
mesh_terms: D000535:Aluminum / Q000633:toxicity*; D000818:Animals; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005786:Gene Expression Regulation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008668:Metallothionein / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012333:RNA, Messenger; D008668:Metallothionein; D000535:Aluminum
keywords: Aluminium; hepatotoxicity; metallothionein genes induction; rats; redox status