title: Synthetic Peptide-Based Antibody Detection for Diagnosis of Chikungunya Infection with and without Neurological Complications.
abstract: Synthetic peptide-based diagnosis of Chikungunya can be an efficient and more accessible approach in immunodiagnostics. Here, we describe the identification of Chikungunya-specific 40 kD protein for development of synthetic peptide-based enzyme-linked immunosorbent assay for the detection of Chikungunya virus-specific antibodies in the patient's sample. The total sodium dodecyl sulfate-polyacrylamide gel electrophoresis protein profile of the patient's sample can be done to identify specific protein bands. The identified proteins can be subjected to liquid chromatography-tandem mass spectrometry (LC-MS/MS) for characterization. After characterization, immunogenic peptides can be designed using softwares and subsequently synthesized chemically. The peptides can be used to develop more specific, sensitive, and simpler diagnostic assay.
authors.affiliation: Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Nagpur, 440010, India. raj_ciims@rediffmail.com.
authors.forename: Rajpal S
authors.initials: RS
authors.lastname: Kashyap
authors.affiliation: Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Nagpur, 440010, India.
authors.forename: Shradha S
authors.initials: SS
authors.lastname: Bhullar
authors.affiliation: Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Nagpur, 440010, India.
authors.forename: Nitin H
authors.initials: NH
authors.lastname: Chandak
authors.affiliation: Biochemistry Research Laboratory, Central India Institute of Medical Sciences, Nagpur, 440010, India.
authors.forename: Girdhar M
authors.initials: GM
authors.lastname: Taori
mesh_terms: D000914:Antibodies, Viral / Q000378:metabolism*; D065632:Chikungunya Fever / Q000175:diagnosis* / Q000276:immunology; D002646:Chikungunya virus / Q000276:immunology*; D002853:Chromatography, Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology*; D012680:Sensitivity and Specificity; D012984:Software; D053719:Tandem Mass Spectrometry; D014764:Viral Proteins / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D010455:Peptides; D014764:Viral Proteins
keywords: Enzyme linked-immunosorbent assay; LC-MS/MS; One-dimensional electrophoresis; Synthetic peptide; Two-dimensional electrophoresis