title: Shear Wave Imaging of Passive Diastolic Myocardial Stiffness: Stunned Versus Infarcted Myocardium.
abstract: The aim of this study was to investigate the potential of shear wave imaging (SWI), a novel ultrasound-based technique, to noninvasively quantify passive diastolic myocardial stiffness in an ovine model of ischemic cardiomyopathy.
  
  
  
  Evaluation of diastolic left ventricular function is critical for evaluation of heart failure and ischemic cardiomyopathy. Myocardial stiffness is known to be an important property for the evaluation of the diastolic myocardial function, but this parameter cannot be measured noninvasively by existing techniques.
  
  
  
  SWI was performed in vivo in open-chest procedures in 10 sheep. Ligation of a diagonal of the left anterior descending coronary artery was performed for 15 min (stunned group, n = 5) and 2 h (infarcted group, n = 5). Each procedure was followed by a 40-min reperfusion period. Diastolic myocardial stiffness was measured at rest, during ischemia, and after reperfusion by using noninvasive shear wave imaging. Simultaneously, end-diastolic left ventricular pressure and segmental strain were measured with a pressure catheter and sonomicrometers during transient vena caval occlusions to obtain gold standard evaluation of myocardial stiffness using end-diastolic strain-stress relationship (EDSSR).
  
  
  
  In both groups, the end-systolic circumferential strain was drastically reduced during ischemia (from 14.2 ± 1.2% to 1.3 ± 1.6% in the infarcted group and from 13.5 ± 3.0% to 1.9 ± 1.8% in the stunned group; p <0.01). SWI diastolic stiffness increased after 2 h of ischemia from 1.7 ± 0.4 to 6.2 ± 2.2 kPa (p < 0.05) and even more after reperfusion (12.1 ± 4.2 kPa; p < 0.01). Diastolic myocardial stiffening was confirmed by the exponential constant coefficient of the EDSSR, which increased from 8.8 ± 2.3 to 25.7 ± 9.5 (p < 0.01). In contrast, SWI diastolic stiffness was unchanged in the stunned group (2.3 ± 0.4 kPa vs 1.8 ± 0.3 kPa, p = NS) which was confirmed also by the exponential constant of EDSSR (9.7 ± 3.1 vs 10.2 ± 2.3, p = NS).
  
  
  
  Noninvasive SWI evaluation of diastolic myocardial stiffness can differentiate between stiff, noncompliant infarcted wall and softer wall containing stunned myocardium.
authors.affiliation: Institut Langevin, Ecole Supérieure de Physique Chimie Industrielles de Paris, ParisTech, Paris, France; CNRS, Unité mixte de recherche 7587, Paris, France; Institut National de la Santé et de la Recherche Médicale, U979, Paris, France; Université Paris Diderot-Paris7, Paris, France. Electronic address: mathieu.pernot@inserm.fr.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Pernot
authors.affiliation: Institut Langevin, Ecole Supérieure de Physique Chimie Industrielles de Paris, ParisTech, Paris, France; CNRS, Unité mixte de recherche 7587, Paris, France; Institut National de la Santé et de la Recherche Médicale, U979, Paris, France; Université Paris Diderot-Paris7, Paris, France.
authors.forename: Wei-Ning
authors.initials: WN
authors.lastname: Lee
authors.affiliation: Hopital Européen Georges Pompidou, Paris, Unité mixte de recherche 970, PARCC, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Bel
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, Unité mixte de recherche-S 769, Châtenay-Malabry, France; Université Paris-Sud, Faculté de Pharmacie, Châtenay-Malabry, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Mateo
authors.affiliation: SuperSonic Imagine, Aix-en-Provence, France.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Couade
authors.affiliation: Institut Langevin, Ecole Supérieure de Physique Chimie Industrielles de Paris, ParisTech, Paris, France; CNRS, Unité mixte de recherche 7587, Paris, France; Institut National de la Santé et de la Recherche Médicale, U979, Paris, France; Université Paris Diderot-Paris7, Paris, France.
authors.forename: Mickaël
authors.initials: M
authors.lastname: Tanter
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, Unité mixte de recherche-S 769, Châtenay-Malabry, France; Université Paris-Sud, Faculté de Pharmacie, Châtenay-Malabry, France.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Crozatier
authors.affiliation: Hopital Européen Georges Pompidou, Paris, Unité mixte de recherche 970, PARCC, France; Université Paris Descartes, Faculté de Médecine, Paris, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Messas
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D009202:Cardiomyopathies / Q000000981:diagnostic imaging* / Q000503:physiopathology; D003971:Diastole; D004195:Disease Models, Animal; D055119:Elastic Modulus; D009203:Myocardial Infarction / Q000000981:diagnostic imaging* / Q000503:physiopathology; D017682:Myocardial Stunning / Q000000981:diagnostic imaging* / Q000503:physiopathology; D011237:Predictive Value of Tests; D034561:Sheep, Domestic; D013997:Time Factors; D014463:Ultrasonography / Q000379:methods*; D016277:Ventricular Function, Left; D017725:Ventricular Pressure
keywords: echocardiography; elasticity; imaging; ischemia; myocardial stiffness; myocardium