title: Intestinal immunomodulating activity and structural characterization of a new polysaccharide from stems of Dendrobium officinale.
abstract: A homogeneous polysaccharide fraction (DOP-W3-b) with a high intestinal immunomodulating activity was obtained from the stems of Dendrobium officinale through a bioactivity-guided sequential isolation procedure based on the screening of Peyer's patch-mediated immunomodulating activity. Oral administration experiments of mice showed that DOP-W3-b could effectively regulate intestinal mucosal immune activity by changing intestinal mucosal structures, promoting the secretions of cytokines from Peyer's patches (PPs) and mesenteric lymph nodes (MLNs), and increasing the production of secretory immunoglobulin A (sIgA) in the lamina propria. Structure analysis indicated that DOP-W3-b was composed of mannose and glucose in a molar ratio of 4.5 with a relatively low molecular weight of 1.543 × 10(4) Da, and its repeat unit contained a backbone consisting of β-(1→4)-d-Manp, β-(1→4)-d-Glcp and β-(1→3,6)-d-Manp residues, a branch consisting of β-(1→4)-d-Manp, β-(1→4)-d-Glcp and terminal β-d-Glcp, and O-acetyl groups attached to O-2 of β-(1→4)-d-Manp. These results suggested that DOP-W3-b was a new polysaccharide with an essential potential for modulating body's immune functions.
authors.affiliation: School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China. jianpingluo@hfut.edu.cn.
authors.forename: Song-Zi
authors.initials: SZ
authors.lastname: Xie
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Zhang
authors.forename: Xue-Qiang
authors.initials: XQ
authors.lastname: Zha
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Pan
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Luo
mesh_terms: D000284:Administration, Oral; D000818:Animals; D031667:Dendrobium / Q000737:chemistry*; D005260:Female; D005947:Glucose / Q000378:metabolism; D007071:Immunoglobulin A, Secretory / Q000378:metabolism; D056747:Immunomodulation; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism; D008198:Lymph Nodes / Q000187:drug effects / Q000378:metabolism; D008358:Mannose / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D023421:Models, Animal; D008970:Molecular Weight; D010581:Peyer's Patches / Q000187:drug effects / Q000378:metabolism; D018547:Plant Stems / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D007071:Immunoglobulin A, Secretory; D011134:Polysaccharides; D005947:Glucose; D008358:Mannose