title: [CHARACTERISTICS OF BIOLOGICAL AND MOLECULAR-GENETIC PROPERTIES OF LACTOBACILLUS FERMENTUM 90 TC-4 PROBIOTIC STRAIN].
abstract: OBJECTIVE
  Confirmation of taxonomic position of Lactobacillus fermentum 90 TC-4 strain using phenotypic (classic microbiological, MALDI TOF mass-spectrometry) and genetic (16S rRNA gene segment sequencing and full genome sequencing) methods.
  
  
  METHODS
  Object of the study--Lactobacillus fermentum 90 TC-4 strains from various collections. Mass-spectrometric analysis was carried out using Autoflex MALDI TOF mass-spectrometer (Bruker Daltonics, Germany), study of biochemical properties of the strain was carried out using API 50 CHL strips (Biomerueux, France), "DNA-sorb B" kitwas used for isolation ofgenome DNA (CRIE, Moscow). Sequencing of the accumulated fragments of 16S rRNA gene was carried out using GenomeLab GeXP sequencing (Beckman Coulter, USA), full genome sequencing was carried out in MiSeq platform (Illumina). Assembly of genome and bioinformation analysis was carried out using BLAST program (www.blast.ncbi.nlm.nih.gov/blast.cgi), "CLC Bio Assembly" and genome server RAST (rast.nmpdr.org).
  
  
  RESULTS
  L. fermentum 90 TC-4 strain was established to be contaminated by L. plantarum culture in a series of cases. As a result of identification of a pure culture of L. fermentum 90 TC-4 strain using a specter of high-technology methods, membership of the strain in L. fer- mentum species has been proven.
  
  
  CONCLUSIONS
  Taxonomic status of L. fermentum 90 TC-4 strain was confirmed.
authors.forename: A G
authors.initials: AG
authors.lastname: Tochilina
authors.forename: I V
authors.initials: IV
authors.lastname: Belova
authors.forename: I V
authors.initials: IV
authors.lastname: Solovieva
authors.forename: I S
authors.initials: IS
authors.lastname: Gorlova
authors.forename: T P
authors.initials: TP
authors.lastname: Ivanova
authors.forename: V A
authors.initials: VA
authors.lastname: Zhirnov
mesh_terms: D001483:Base Sequence; D016680:Genome, Bacterial; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D052197:Limosilactobacillus fermentum / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D019936:Probiotics; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D012336:RNA, Ribosomal, 16S