title: Modeling Murine Gastric Metaplasia Through Tamoxifen-Induced Acute Parietal Cell Loss.
abstract: Parietal cell loss represents the initial step in the sequential progression toward gastric adenocarcinoma. In the setting of chronic inflammation, the expansion of the mucosal response to parietal cell loss characterizes a crucial transition en route to gastric dysplasia. Here, we detail methods for using the selective estrogen receptor modulator tamoxifen as a novel tool to rapidly and reversibly induce parietal cell loss in mice in order to study the mechanisms that underlie these pre-neoplastic events.
authors.affiliation: Division of Gastroenterology, Departments of Medicine, Washington University School of Medicine, Box 8124, 660 So. Euclid Ave., St. Louis, MO, 63110, USA.
authors.forename: Jose B
authors.initials: JB
authors.lastname: Saenz
authors.affiliation: Division of Gastroenterology, Departments of Medicine, Washington University School of Medicine, Box 8124, 660 So. Euclid Ave., St. Louis, MO, 63110, USA.|Division of Gastroenterology, Departments of Developmental Biology, Washington University School of Medicine, St. Louis, MO, 63110, USA.
authors.forename: Joseph
authors.initials: J
authors.lastname: Burclaff
authors.affiliation: Division of Gastroenterology, Departments of Medicine, Washington University School of Medicine, Box 8124, 660 So. Euclid Ave., St. Louis, MO, 63110, USA. jmills@wustl.edu.|Division of Gastroenterology, Departments of Developmental Biology, Washington University School of Medicine, St. Louis, MO, 63110, USA. jmills@wustl.edu.|Division of Gastroenterology, Departments of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO, 63110, USA. jmills@wustl.edu.
authors.forename: Jason C
authors.initials: JC
authors.lastname: Mills
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D007267:Injections; D008679:Metaplasia; D051379:Mice; D010295:Parietal Cells, Gastric / Q000187:drug effects / Q000473:pathology*; D011230:Precancerous Conditions / Q000139:chemically induced* / Q000473:pathology; D013274:Stomach Neoplasms / Q000139:chemically induced* / Q000473:pathology; D013629:Tamoxifen / Q000008:administration & dosage / Q000633:toxicity*
chemical_list: D013629:Tamoxifen
keywords: Metaplasia; Oxyntic atrophy; Parietal cell loss; Spasmolytic polypeptide-expressing metaplasia (SPEM); Tamoxifen