title: Native plasmids restrict growth of Phaeobacter inhibens DSM 17395: Energetic costs of plasmids assessed by quantitative physiological analyses.
abstract: Plasmid carriage is associated with energetic costs, and thus only those plasmids providing fitness benefits are stably maintained in the host lineage. Marine bacteria of the Roseobacter clade harbor up to 11 extrachromosomal replicons, adding lifestyle-relevant and possibly habitat success-promoting functions to their genomic repertoire. Phaeobacter inhibens DSM 17395 is a nutritionally versatile representative, carrying three stable and functionally distinct plasmids (65, 78, and 262 kb). The present study investigates the physiological and energetic consequences of plasmid carriage in P. inhibens DSM 17395, employing mutants cured from all native plasmids in every possible combination (seven different). Cultivation in process-controlled bioreactors with casamino acids as organic substrate revealed a complex physiological response, suggesting existence of functional interconnections between the replicons. Deletion of the 262 kb plasmid boosted growth rate (>3-fold) and growth efficiency (yields for carbon, O2and CO2), which was not observed for the 65 or 78 kb plasmid. Carriage of the 262 kb plasmid was most costly for the wild type, i.e. contributing ∼50% to its energetic (dissimilatory) expenditures. Cost-benefit analysis of plasmid carriage reflects the high value of plasmids for niche specialization of P. inhibens DSM 17395 and most likely also for related Phaeobacter species.
authors.affiliation: General and Molecular Microbiology, Institute for Chemistry and Biology of the Marine Environment (ICBM), Carl von Ossietzky University Oldenburg, Oldenburg, Germany.
authors.forename: Kathleen
authors.initials: K
authors.lastname: Trautwein
authors.affiliation: Institute for Biochemistry, Biotechnology and Bioinformatics, Technische Universität Carolo-Wilhelmina Braunschweig, Braunschweig, Germany.
authors.forename: Sabine Eva
authors.initials: SE
authors.lastname: Will
authors.affiliation: General and Molecular Microbiology, Institute for Chemistry and Biology of the Marine Environment (ICBM), Carl von Ossietzky University Oldenburg, Oldenburg, Germany.
authors.forename: Reiner
authors.initials: R
authors.lastname: Hulsch
authors.affiliation: General and Molecular Microbiology, Institute for Chemistry and Biology of the Marine Environment (ICBM), Carl von Ossietzky University Oldenburg, Oldenburg, Germany.
authors.forename: Uwe
authors.initials: U
authors.lastname: Maschmann
authors.affiliation: General and Molecular Microbiology, Institute for Chemistry and Biology of the Marine Environment (ICBM), Carl von Ossietzky University Oldenburg, Oldenburg, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Wiegmann
authors.affiliation: Institute for Biochemistry, Biotechnology and Bioinformatics, Technische Universität Carolo-Wilhelmina Braunschweig, Braunschweig, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Hensler
authors.affiliation: Department Microbial Ecology and Diversity Projects, Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.
authors.forename: Victoria
authors.initials: V
authors.lastname: Michael
authors.affiliation: General and Molecular Microbiology, Institute for Chemistry and Biology of the Marine Environment (ICBM), Carl von Ossietzky University Oldenburg, Oldenburg, Germany.
authors.forename: Hanna
authors.initials: H
authors.lastname: Ruppersberg
authors.affiliation: General and Molecular Microbiology, Institute for Chemistry and Biology of the Marine Environment (ICBM), Carl von Ossietzky University Oldenburg, Oldenburg, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Wünsch
authors.affiliation: Mathematical Modelling, Institute for Chemistry and Biology of the Marine Environment (ICBM), Carl von Ossietzky University Oldenburg, Oldenburg, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Feenders
authors.affiliation: Institute for Biochemistry, Biotechnology and Bioinformatics, Technische Universität Carolo-Wilhelmina Braunschweig, Braunschweig, Germany.
authors.forename: Meina
authors.initials: M
authors.lastname: Neumann-Schaal
authors.affiliation: Institute for Biochemistry, Biotechnology and Bioinformatics, Technische Universität Carolo-Wilhelmina Braunschweig, Braunschweig, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Kaltenhäuser
authors.affiliation: Institute for Biochemistry, Biotechnology and Bioinformatics, Technische Universität Carolo-Wilhelmina Braunschweig, Braunschweig, Germany.
authors.forename: Marcus
authors.initials: M
authors.lastname: Ulbrich
authors.affiliation: Institute for Biochemistry, Biotechnology and Bioinformatics, Technische Universität Carolo-Wilhelmina Braunschweig, Braunschweig, Germany.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Schmidt-Hohagen
authors.affiliation: Mathematical Modelling, Institute for Chemistry and Biology of the Marine Environment (ICBM), Carl von Ossietzky University Oldenburg, Oldenburg, Germany.
authors.forename: Bernd
authors.initials: B
authors.lastname: Blasius
authors.affiliation: Department Microbial Ecology and Diversity Projects, Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.
authors.forename: Jörn
authors.initials: J
authors.lastname: Petersen
authors.affiliation: Institute for Biochemistry, Biotechnology and Bioinformatics, Technische Universität Carolo-Wilhelmina Braunschweig, Braunschweig, Germany.
authors.forename: Dietmar
authors.initials: D
authors.lastname: Schomburg
authors.affiliation: General and Molecular Microbiology, Institute for Chemistry and Biology of the Marine Environment (ICBM), Carl von Ossietzky University Oldenburg, Oldenburg, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Rabus
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D004734:Energy Metabolism; D010957:Plasmids; D012093:Replicon; D041902:Rhodobacteraceae / Q000235:genetics* / Q000254:growth & development; D041904:Roseobacter / Q000235:genetics
chemical_list: D000596:Amino Acids; C017721:casamino acids