title: Modulation of the E. coli rpoH Temperature Sensor with Triptycene-Based Small Molecules.
abstract: Regulation of the heat shock response (HSR) is essential in all living systems. In E. coli, the HSR is regulated by an alternative σ factor, σ(32) , which is encoded by the rpoH gene. The mRNA of rpoH adopts a complex secondary structure that is critical for the proper translation of the σ(32) protein. At low temperatures, the rpoH gene transcript forms a highly structured mRNA containing several three-way junctions, including a rare perfectly paired three-way junction (3WJ). This complex secondary structure serves as a primitive but highly effective strategy for the thermal control of gene expression. In this work, the first small-molecule modulators of the E. coli σ(32) mRNA temperature sensor are reported.
authors.affiliation: Department of Chemistry, University of Pennsylvania, 231 South 34th Street, Philadelphia, PA, 19104, USA.
authors.forename: Stephanie A
authors.initials: SA
authors.lastname: Barros
authors.affiliation: Department of Chemistry, University of Pennsylvania, 231 South 34th Street, Philadelphia, PA, 19104, USA.
authors.forename: Ina
authors.initials: I
authors.lastname: Yoon
authors.affiliation: Department of Chemistry, University of Pennsylvania, 231 South 34th Street, Philadelphia, PA, 19104, USA. dcheno@sas.upenn.edu.
authors.forename: David M
authors.identifier: 0000-0002-0819-4669
authors.initials: DM
authors.lastname: Chenoweth
mesh_terms: D000873:Anthracenes / Q000737:chemistry / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013696:Temperature
chemical_list: D000873:Anthracenes; D012329:RNA, Bacterial; D012333:RNA, Messenger; C520712:triptycene
keywords: RNA; heat shock; temperature sensors; triptycenes