title: In vitro selection of antibiotic-binding aptamers.
abstract: Despite its wide applicability the selection of small molecule-binding RNA aptamers with high affinity binding and specificity is still challenging. We will present here a protocol which allows the in vitro selection of antibiotic-binding aptamers which turned out to be important building blocks for the design process of synthetic riboswitches. The presented methods will be compared with alternative in vitro selection protocols. A detailed note section will point out useful tips and pitfalls.
authors.affiliation: Department of Biology, Technical University Darmstadt, Schnittspahnstr. 10, 64287 Darmstadt, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Groher
authors.affiliation: Department of Biology, Technical University Darmstadt, Schnittspahnstr. 10, 64287 Darmstadt, Germany. Electronic address: bsuess@bio.tu-darmstadt.de.
authors.forename: Beatrix
authors.initials: B
authors.lastname: Suess
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry*; D052157:Aptamers, Nucleotide / Q000737:chemistry / Q000235:genetics*; D058928:Riboswitch / Q000235:genetics; D052156:SELEX Aptamer Technique / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D052157:Aptamers, Nucleotide; D058928:Riboswitch
keywords: Biotechnology; Combinatorial chemistry; In vitro selection; RNA aptamer; SELEX