title: Apigenin-7-O-β-D-glycoside isolation from the highly copper-tolerant plant Elsholtzia splendens.
abstract: Elsholtzia splendens (Lamiaceae) is a copper-tolerant plant species growing on copper deposits in the south of China. Chromatographic separation of n-BuOH extracts from the flowering aerial biomass afforded apigenin-7-O-β-D-glycoside, using macroporous resin, Sephadex™ LH-20 gel, polyamide resin as well as preparative high-performance liquid chromatography (P-HPLC) columns. Chemical structure was elucidated using HPLC/ESI-MS (electrospray ionization-mass spectrometry), Fourier transform infrared (FTIR), and (1)D- and (2)D-nuclear magnetic resonance (NMR). Apigenin-7-O-β-D-glycoside could be the post-harvesting product from E. splendens biomass.
authors.affiliation: Key Laboratory of Environmental Remediation and Ecological Health, Ministry of Education, College of Environmental & Resource Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Hong-Yun
authors.initials: HY
authors.lastname: Peng
authors.affiliation: Key Laboratory of Environmental Remediation and Ecological Health, Ministry of Education, College of Environmental & Resource Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Xue-Hong
authors.initials: XH
authors.lastname: Zhang
authors.affiliation: Institution of Marine Biology, Ocean College, Zhejiang University, Hangzhou 310058, China.
authors.forename: Jin-Zhong
authors.initials: JZ
authors.lastname: Xu
mesh_terms: D047310:Apigenin / Q000737:chemistry / Q000302:isolation & purification*; D003300:Copper / Q000494:pharmacology*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification*; D019686:Lamiaceae / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D006027:Glycosides; D003300:Copper; D047310:Apigenin
keywords: Chromatographic separation; Copper-tolerant plant; Elsholtzia splendens; Flavonoid glycoside; Structure elucidation