title: Increased susceptibility to pentylenetetrazol following survival of cerebral malaria in mice.
abstract: Malaria is considered a neglected disease and public health problem, affecting >200 million people worldwide. In the present study we used the Plasmodium berghei ANKA (PbA) model of experimental cerebral malaria (CM) in C57BL/6 mice. After rescue from CM and parasite clearance, animals were submitted to a seizure susceptibility test (45 days after infection) using a low dose of pentylenetetrazol (PTZ, 30 mg/kg) and monitored with use of behavioral and electroencephalography (EEG) methods. Mice rescued from CM presented a reduced latency to myoclonic and tonic-clonic seizures and an increased duration of tonic-clonic seizures. In addition, quantitative analysis of EEG revealed a decrease in relative power at beta frequency band in PbA-infected animals after PTZ injection. Our results suggest that CM may lead to increased susceptibility to seizures in mice.
authors.affiliation: Graduate Program in Pharmacology and Department of Physiology and Pharmacology, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.
authors.forename: Ana C B
authors.initials: AC
authors.lastname: Grauncke
authors.affiliation: Graduate Program in Pharmacology and Department of Physiology and Pharmacology, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.
authors.forename: Thaíze L
authors.initials: TL
authors.lastname: Souza
authors.affiliation: Graduate Program in Pharmacology and Department of Physiology and Pharmacology, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.
authors.forename: Leandro R
authors.initials: LR
authors.lastname: Ribeiro
authors.affiliation: Graduate Program in Health Sciences: Infectious Diseases and Tropical Medicine and Department of Biochemistry and Immunology, Institute of Biological Science, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Fátima
authors.initials: F
authors.lastname: Brant
authors.affiliation: Graduate Program in Health Sciences: Infectious Diseases and Tropical Medicine and Department of Biochemistry and Immunology, Institute of Biological Science, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Fabiana S
authors.initials: FS
authors.lastname: Machado
authors.affiliation: Graduate Program in Pharmacology and Department of Physiology and Pharmacology, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.
authors.forename: Mauro S
authors.initials: MS
authors.lastname: Oliveira
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D003292:Convulsants / Q000009:adverse effects*; D004195:Disease Models, Animal; D004198:Disease Susceptibility / Q000139:chemically induced*; D004569:Electroencephalography; D004827:Epilepsy / Q000139:chemically induced*; D016779:Malaria, Cerebral / Q000188:drug therapy; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010433:Pentylenetetrazole / Q000009:adverse effects*; D010962:Plasmodium berghei / Q000472:pathogenicity; D018709:Statistics, Nonparametric; D013997:Time Factors
chemical_list: D003292:Convulsants; D010433:Pentylenetetrazole
keywords: Electroencephalography; Epilepsy; Pentylenetetrazol; Plasmodium berghei 
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