title: Curculigoside augments cell-mediated immune responses in metastatic tumor-bearing animals.
abstract: A positive modulation of immune system is necessary for preparing the body to fight against malignant tumor cells. In the present study, the stimulatory effect of Curculigoside on cell-mediated immune response against the metastasis of B16F10 melanoma cells was analyzed in C57BL/6 mice. Curculigoside is a phenolic glucoside present in the plant Curculigo orchioides Gaertn. (Family - Amaryllidaceae). Administration of Curculigoside enhanced the natural killer (NK) cell activity, antibody-dependent cell-mediated cytotoxicity and complement-mediated cytotoxicity in metastatic tumor-bearing animals, when compared to the untreated control animals. The compound was also found to be effective in reducing the levels of proinflammatory cytokines such as TNF-α, IL-1β, IL-6 and GM-CSF during metastasis. Besides these, levels of TH1 cytokines, such as IL-2 and IFN-γ, were significantly enhanced (p < 0.001) by Curculigoside administration and thereby reduces the metastatic lung colony formation along with an increased lifespan of the experimental animals. These studies provide an evidence for the stimulation of cell-mediated immune responses by Curculigoside against B16F10-induced metastatic tumor progression in experimental animals.
authors.affiliation: a Department of Immunology , Amala Cancer Research Centre, Affiliated to the University of Calicut , Thrissur , Kerala State , India.
authors.forename: Vishnu Priya
authors.initials: VP
authors.lastname: Murali
authors.affiliation: a Department of Immunology , Amala Cancer Research Centre, Affiliated to the University of Calicut , Thrissur , Kerala State , India.
authors.forename: Girija
authors.initials: G
authors.lastname: Kuttan
mesh_terms: D000818:Animals; D001565:Benzoates / Q000737:chemistry / Q000494:pharmacology*; D031408:Curculigo / Q000737:chemistry*; D016207:Cytokines / Q000276:immunology; D005960:Glucosides / Q000737:chemistry / Q000494:pharmacology*; D064593:Heterografts; D006801:Humans; D007111:Immunity, Cellular / Q000187:drug effects*; D020014:K562 Cells; D008297:Male; D051379:Mice; D009362:Neoplasm Metastasis; D009374:Neoplasms, Experimental / Q000276:immunology* / Q000473:pathology
chemical_list: D001565:Benzoates; D016207:Cytokines; D005960:Glucosides; C477170:curculigoside
keywords: Antibody-dependent cellular cytotoxicity; TH1 cytokines; antibody-dependent complement-mediated cytotoxicity; cell-mediated immunity; natural killer cells; proinflammatory cytokines