title: Antibodies reacting to carbonic anhydrase isozymes (I and II) and albumin in sera from dogs.
abstract: IgGs to carbonic anhydrase isozymes (CA-I and CA-II) and albumin were identified in dog serum. IgG titers were determined in the sera of asymptomatic dogs, and in dogs with atopic dermatitis, diarrhea and/or vomiting, diabetes and/or pancreatitis, kidney disease, hepatic disease, and thyroid gland disease, using ELISA. Low titres of IgG-reactive CA-I, CA-II, BSA, and CSA were found in the sera of healthy beagles. Compared with healthy beagles, there was a significant difference in the titers of antibodies against CA-I in asymptomatic dogs, dogs with diabetes and/or pancreatitis, or thyroid gland disease, or hepatic disease. Compared with healthy beagles, there was a significant difference in the antibody titer of anti-CA-II IgG in asymptomatic dogs and in those with hepatic disease. There was a significant difference in the antibody titer of anti-BSA IgG between healthy beagles and dogs with hepatic disease.
authors.affiliation: Laboratory of Physiology I, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa 252-5201, Japan. Electronic address: nishida@azabu-u.ac.jp.
authors.forename: Toshiho
authors.initials: T
authors.lastname: Nishita
authors.affiliation: Laboratory of Physiology I, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa 252-5201, Japan.
authors.forename: Rui
authors.initials: R
authors.lastname: Miyazaki
authors.affiliation: Laboratory of Physiology I, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa 252-5201, Japan.
authors.forename: Takae
authors.initials: T
authors.lastname: Miyazaki
authors.affiliation: Research Institute of Biosciences, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa 252-5201, Japan.
authors.forename: Hideharu
authors.initials: H
authors.lastname: Ochiai
authors.affiliation: Laboratory of Physiology II, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa 252-5201, Japan.
authors.forename: Kensuke
authors.initials: K
authors.lastname: Orito
mesh_terms: D000418:Albumins / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D015551:Autoimmunity; D024401:Carbonic Anhydrase I / Q000235:genetics* / Q000378:metabolism; D024402:Carbonic Anhydrase II / Q000235:genetics* / Q000378:metabolism; D004283:Dog Diseases / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D004285:Dogs / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005512:Food Hypersensitivity / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000662:veterinary; D007074:Immunoglobulin G / Q000097:blood*; D008297:Male
chemical_list: D000418:Albumins; D007074:Immunoglobulin G; D024401:Carbonic Anhydrase I; D024402:Carbonic Anhydrase II
keywords: Antibody; Bovine serum albumin; Carbonic anhydrase; Dog; Food allergy