title: Silencing of HMGA2 promotes apoptosis and inhibits migration and invasion of prostate cancer cells.
abstract: The high mobility group protein A2 (HMGA2) has been demonstrated as an architectural transcription factor that is associated with pathogenesis of many malignant cancers; however, its role in prostate cancer cells remains largely unknown. To explore whether HMGA2 participates in the development and progression of prostate cancer, small interfering RNA (siRNA) targeted on human HMGA2 was transfected to suppress the HMGA2 expression in prostate cancer PC3 and DU145 cells, and then the cellular biology changes after decreased the expression of HMGA2 was examined. Our results showed that knockdown of HMGA2 markedly inhibited cell proliferation; this reduced cell proliferation was due to the promotion of cell apoptosis as the Bcl-xl was decreased, whereas Bax was up-regulated. In addition, we found that HMGA2 knockdown resulted in reduction of cell migration and invasion, as well as repressed the expression of matrix metalloproteinases (MMPs) and affected the occurrence of epithelial-mesenchymal transition (EMT) in both cell types. We further found that decreased HMGA2 expression inhibited the transforming growth factor-beta (TGF-beta)/Smad signalling pathway in cancer cells. In conclusion, our data indicated that HMGA2 was associated with apoptosis, migration and invasion of prostate cancer, which might be a promising therapeutic target for prostate cancer.
authors.affiliation: Department of Urology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002 Jiangsu Province, China.
authors.forename: Zhan
authors.initials: Z
authors.lastname: Shi
authors.forename: Ding
authors.initials: D
authors.lastname: Wu
authors.forename: Run
authors.initials: R
authors.lastname: Tang
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.forename: Renfu
authors.initials: R
authors.lastname: Chen
authors.forename: Song
authors.initials: S
authors.lastname: Xue
authors.forename: Chengjing
authors.initials: C
authors.lastname: Zhang
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Sun
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D025743:HMGA2 Protein / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D008297:Male; D009361:Neoplasm Invasiveness / Q000235:genetics; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D034741:RNA, Small Interfering; D015398:Signal Transduction; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis / Q000235:genetics; D051020:bcl-X Protein / Q000096:biosynthesis / Q000235:genetics
chemical_list: C494826:BAX protein, human; C494811:BCL2L1 protein, human; D025743:HMGA2 Protein; D034741:RNA, Small Interfering; D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein