title: Fibronectin on circulating extracellular vesicles as a liquid biopsy to detect breast cancer.
abstract: Extracellular vesicles (EVs) secreted from cancer cells have potential for generating cancer biomarker signatures. Fibronectin (FN) was selected as a biomarker candidate, due to the presence in surface on EVs secreted from human breast cancer cell lines. A subsequent study used two types of enzyme-linked immunosorbent assays (ELISA) to determine the presence of these proteins in plasma samples from disease-free individuals (n=70), patients with BC (n=240), BC patients after surgical resection (n=40), patients with benign breast tumor (n=55), and patients with non-cancerous diseases (thyroiditis, gastritis, hepatitis B, and rheumatoid arthritis; n=80). FN levels were significantly elevated (p< .0001) at all stages of BC, and returned to normal after tumor removal. The diagnostic accuracy for FN detection in extracellular vesicles (ELISA method 1) (area under the curve, 0.81; 95% CI, 0.76 to 0.86; sensitivity of 65.1% and specificity of 83.2%) were also better than those for FN detection in the plasma (ELISA method 2) (area under the curve, 0.77; 95% CI, 0.72 to 0.83; sensitivity of 69.2% and specificity of 73.3%) in BC. The diagnostic accuracy of plasma FN was similar in both the early-stage BC and all BC patients, as well as in the two sets. This liquid biopsy to detect FN on circulating EVs could be a promising method to detect early breast cancer.
authors.affiliation: Department of Molecular Medicine, Cell and Matrix Research Institute, School of Medicine, Kyungpook National University, Daegu 700-422, Republic of Korea.
authors.forename: Pyong-Gon
authors.initials: PG
authors.lastname: Moon
authors.affiliation: Department of Molecular Medicine, Cell and Matrix Research Institute, School of Medicine, Kyungpook National University, Daegu 700-422, Republic of Korea.
authors.forename: Jeong-Eun
authors.initials: JE
authors.lastname: Lee
authors.affiliation: Department of Molecular Medicine, Cell and Matrix Research Institute, School of Medicine, Kyungpook National University, Daegu 700-422, Republic of Korea.
authors.forename: Young-Eun
authors.initials: YE
authors.lastname: Cho
authors.affiliation: Department of Oncology/Hematology, Kyungpook National University Hospital, Daegu 700-721, Republic of Korea.
authors.forename: Soo Jung
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Department of Oncology/Hematology, Kyungpook National University Hospital, Daegu 700-721, Republic of Korea.
authors.forename: Yee Soo
authors.initials: YS
authors.lastname: Chae
authors.affiliation: Department of Breast & Thyroid Surgery, Kyungpook National University Hospital, Daegu 700-721, Republic of Korea.
authors.forename: Jin Hyang
authors.initials: JH
authors.lastname: Jung
authors.affiliation: Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, KU-KIST School, Korea University, Seoul 02841, Republic of Korea.
authors.forename: In-San
authors.initials: IS
authors.lastname: Kim
authors.affiliation: Department of Breast & Thyroid Surgery, Kyungpook National University Hospital, Daegu 700-721, Republic of Korea.
authors.forename: Ho Yong
authors.initials: HY
authors.lastname: Park
authors.affiliation: Department of Molecular Medicine, Cell and Matrix Research Institute, School of Medicine, Kyungpook National University, Daegu 700-422, Republic of Korea.
authors.forename: Moon-Chang
authors.initials: MC
authors.lastname: Baek
mesh_terms: D000328:Adult; D000368:Aged; D019540:Area Under Curve; D001172:Arthritis, Rheumatoid / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000378:metabolism; D001706:Biopsy; D001943:Breast Neoplasms / Q000378:metabolism*; D016022:Case-Control Studies; D045744:Cell Line, Tumor; D018572:Disease-Free Survival; D004797:Enzyme-Linked Immunosorbent Assay; D000067128:Extracellular Vesicles / Q000378:metabolism; D005260:Female; D005353:Fibronectins / Q000378:metabolism*; D005756:Gastritis / Q000378:metabolism; D006509:Hepatitis B / Q000378:metabolism; D006801:Humans; D000073890:Liquid Biopsy; D008875:Middle Aged; D015203:Reproducibility of Results; D013966:Thyroiditis / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; C067625:FN1 protein, human; D005353:Fibronectins
keywords: ELISA; breast cancer; diagnosis; extracellular vesicle