title: Effect of dietary organic selenium on muscle proteolytic activity and water-holding capacity in pork.
abstract: This study evaluates the effect of dietary selenium (Se) supplementation source (organic, Se-enriched yeast; SY vs. inorganic, sodium selenite; SS), dose (0.2: L vs. 0.4: H mg/kg) and the combination of Se and vitamin E (VITE+SS) for 26days on drip loss, TBARS, colour changes, myofibrillar protein pattern and proteolysis in pork. The lowest water losses were observed in the SY-H group when compared to the others. SY-H and VITE+SS groups presented lower myofibrillar protein hydrolysis/oxidation. VITE+SS supplementation also resulted in higher PRO, TRP and PHE content at days 2 and 7, whereas the SY group showed increased GLY and CAR and tended to have higher TAU and ANS at day 2. The myofibrillar fragmentation index was not modified by the dietary treatment; however, at day 8, it tended to be higher in groups supplemented with SeY and VITE+SS. The results of the present study might indicate a possible relation between muscle proteolysis and water loss.
authors.affiliation: Incarlopsa, Ctra. N-400 km. 95400, 16400 Tarancón, Cuenca, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Calvo
authors.affiliation: Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Av Agustín Escardino 7, 46980 Paterna, Valencia, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Toldrá
authors.affiliation: Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Av Agustín Escardino 7, 46980 Paterna, Valencia, Spain.
authors.forename: M C
authors.initials: MC
authors.lastname: Aristoy
authors.affiliation: Dpto. Producción Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Avda. Puerta de Hierro s/n., 28040 Madrid, Spain.
authors.forename: C J
authors.initials: CJ
authors.lastname: López-Bote
authors.affiliation: Dpto. Producción Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Avda. Puerta de Hierro s/n., 28040 Madrid, Spain. Electronic address: anarey@vet.ucm.es.
authors.forename: A I
authors.initials: AI
authors.lastname: Rey
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D003116:Color; D004032:Diet / Q000662:veterinary*; D019587:Dietary Supplements; D005978:Glutathione / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D018482:Muscle, Skeletal / Q000737:chemistry*; D059748:Proteolysis; D000069466:Red Meat / Q000032:analysis*; D012643:Selenium / Q000008:administration & dosage*; D018038:Sodium Selenite / Q000008:administration & dosage; D013552:Swine; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014810:Vitamin E / Q000008:administration & dosage / Q000032:analysis; D014867:Water / Q000032:analysis*
chemical_list: D017392:Thiobarbituric Acid Reactive Substances; D014867:Water; D014810:Vitamin E; D005978:Glutathione; D012643:Selenium; D018038:Sodium Selenite
keywords: Drip loss; Free-amino acids; Myofibrillar proteins; Organic selenium; TBARS; Vitamin E