title: Chiral liquid chromatography-mass spectrometry (LC-MS/MS) method development for the detection of salbutamol in urine samples.
abstract: A sequential solid-phase extraction (SPE) method was developed and validated using liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) for the detection and quantification of salbutamol enantiomers in porcine urine. Porcine urine samples were hydrolysed with β-glucuronidase/arylsulfatase from Helix pomatia and then subjected to a double solid-phase extraction (SPE) first using the Abs-Elut Nexus SPE and then followed by the Bond Elut Phenylboronic Acid (PBA) SPE. The salbutamol enantiomers were separated using the Astec CHIROBIOTIC™ T HPLC column (3.0mm×100mm; 5μm) maintained at 15°C with a 15min isocratic run at a flow rate of 0.4mL/min. The mobile phase constituted of 5mM ammonium formate in methanol. Salbutamol and salbutamol-tert-butyl-d9 (internal standard, IS) was monitored and quantified with the multiple reaction monitoring (MRM) mode. The method showed good linearity for the range of 0.1-10ng/mL with limit of quantification at 0.3ng/mL. Analysis of the QC samples showed intra- and inter-assay precisions to be less than 5.04%, and recovery ranging from 83.82 to 102.33%.
authors.affiliation: Usains Biomics Laboratory Testing Services Sdn. Bhd., Universiti Sains Malaysia, Suite 016 Ground Floor, Eureka Complex, 11800 USM Minden, Penang, Malaysia. Electronic address: chansuehay@gmail.com.
authors.forename: Sue Hay
authors.initials: SH
authors.lastname: Chan
authors.affiliation: Institute for Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800 USM Minden, Penang, Malaysia. Electronic address: lxl13_inf029@student.usm.my.
authors.forename: Warren
authors.initials: W
authors.lastname: Lee
authors.affiliation: School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 USM Minden, Penang, Malaysia. Electronic address: amzaini@usm.my.
authors.forename: Mohd Zaini
authors.initials: MZ
authors.lastname: Asmawi
authors.affiliation: Usains Biomics Laboratory Testing Services Sdn. Bhd., Universiti Sains Malaysia, Suite 016 Ground Floor, Eureka Complex, 11800 USM Minden, Penang, Malaysia; Institute for Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800 USM Minden, Penang, Malaysia. Electronic address: tansc88@gmail.com.
authors.forename: Soo Choon
authors.initials: SC
authors.lastname: Tan
mesh_terms: D000420:Albuterol / Q000737:chemistry* / Q000652:urine*; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D057230:Limit of Detection; D016014:Linear Models; D015203:Reproducibility of Results; D013237:Stereoisomerism; D013552:Swine; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000420:Albuterol
keywords: Liquid chromatography–mass spectrometry (LC–MS/MS); Method validation; Porcine urine; Salbutamol enantiomer; Solid-phase-extraction (SPE)