title: Bioabsorbable zinc ion induced biphasic cellular responses in vascular smooth muscle cells.
abstract: Bioabsorbable metal zinc (Zn) is a promising new generation of implantable scaffold for cardiovascular and orthopedic applications. In cardiovascular stent applications, zinc ion (Zn(2+)) will be gradually released into the surrounding vascular tissues from such Zn-containing scaffolds after implantation. However, the interactions between vascular cells and Zn(2+) are still largely unknown. We explored the short-term effects of extracellular Zn(2+) on human smooth muscle cells (SMCs) up to 24 h, and an interesting biphasic effect of Zn(2+) was observed. Lower concentrations (<80 μM) of Zn(2+) had no adverse effects on cell viability but promoted cell adhesion, cell spreading, cell proliferation, cell migration, and enhanced the expression of F-actin and vinculin. Cells treated with such lower concentrations of Zn(2+) displayed an elongated shape compared to controls without any treatment. In contrast, cells treated with higher Zn(2+) concentrations (80-120 μM) had opposite cellular responses and behaviors. Gene expression profiles revealed that the most affected functional genes were related to angiogenesis, inflammation, cell adhesion, vessel tone, and platelet aggregation. Results indicated that Zn has interesting concentration-dependent biphasic effects on SMCs with low concentrations being beneficial to cellular functions.
authors.affiliation: Department of Chemical, Biological and Bioengineering, North Carolina A&T State University, Greensboro, NC 27411, USA.|National Science Foundation (NSF)-Engineering Research Center for Revolutionizing Metallic Biomaterials, Greensboro, NC 27411, USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of Biomedical Engineering, Pennsylvania State University, State College, PA 16801, USA.
authors.forename: Nan
authors.initials: N
authors.lastname: Zhao
authors.affiliation: Department of Chemical, Biological and Bioengineering, North Carolina A&T State University, Greensboro, NC 27411, USA.|National Science Foundation (NSF)-Engineering Research Center for Revolutionizing Metallic Biomaterials, Greensboro, NC 27411, USA.
authors.forename: Donghui
authors.initials: D
authors.lastname: Zhu
mesh_terms: D000199:Actins / Q000096:biosynthesis; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000378:metabolism*; D054457:Tissue Scaffolds; D015032:Zinc / Q000494:pharmacology*
chemical_list: D000199:Actins; D015032:Zinc