title: Effects of cysteamine supplementation on the intestinal expression of amino acid and peptide transporters and intestinal health in finishing pigs.
abstract: This study aimed to evaluate the effects of cysteamine supplementation on the expression of jejunal amino acid and peptide transporters and intestinal health in finishing pigs. Sixty barrows were allocated into two experimental diets consisting of a basal control diet supplemented with 0 or 142 mg/kg cysteamine. After 41 days, 10 pigs per treatment were slaughtered. The results showed that cysteamine supplementation increased the apparent digestibility of crude protein (CP) (P < 0.05) and the trypsin activity in jejunal digesta (P < 0.01). Cysteamine supplementation also increased the messenger RNA abundance of SLC7A7, SLC7A9 and SLC15A1, occludin, claudin-1 and zonula occludens protein-1 (P < 0.001) in the jejunum mucosa. Increased glutathione content (P < 0.01) and glutathione peroxidase activity (P < 0.05) and decreased malondialdehyde content (P < 0.01) were observed in pigs receiving cysteamine. Additionally, cysteamine supplementation increased the concentrations of secretory immunoglobulin A (IgA) (P < 0.05), IgM (P < 0.001) and IgG (P < 0.001) in the jejunal mucosa. It is concluded that cysteamine supplementation could influence protein digestion and absorption via increasing trypsin activity, enhancing the digestibility of CP, and promoting the expression of jejunal amino acid and peptide transporters. Moreover, cysteamine improved intestinal integrity, antioxidant capacity and immune function in the jejunum, which were beneficial for intestinal health.
authors.affiliation: College of Animal Science and Technology, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University, Nanjing, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhou
authors.affiliation: College of Animal Science and Technology, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University, Nanjing, China.
authors.forename: Yiqiu
authors.initials: Y
authors.lastname: Luo
authors.affiliation: College of Animal Science and Technology, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University, Nanjing, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University, Nanjing, China.
authors.forename: Jiaolong
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University, Nanjing, China.
authors.forename: Bolin
authors.initials: B
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University, Nanjing, China.
authors.forename: Shen
authors.initials: S
authors.lastname: Xing
authors.affiliation: College of Animal Science and Technology, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University, Nanjing, China.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: College of Animal Science and Technology, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University, Nanjing, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.affiliation: College of Animal Science and Technology, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing Agricultural University, Nanjing, China.
authors.forename: Guanghong
authors.initials: G
authors.lastname: Zhou
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena / Q000187:drug effects* / Q000502:physiology*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D003543:Cysteamine / Q000008:administration & dosage* / Q000494:pharmacology*; D004032:Diet / Q000662:veterinary*; D004044:Dietary Proteins / Q000378:metabolism; D019587:Dietary Supplements; D004063:Digestion / Q000187:drug effects; D007583:Jejunum / Q000201:enzymology / Q000276:immunology / Q000378:metabolism*; D008297:Male; D026901:Membrane Transport Proteins / Q000378:metabolism*; D013552:Swine / Q000378:metabolism* / Q000502:physiology*; D014357:Trypsin / Q000378:metabolism
chemical_list: D000596:Amino Acids; D000975:Antioxidants; D004044:Dietary Proteins; D026901:Membrane Transport Proteins; D003543:Cysteamine; C043912:peptide permease; D014357:Trypsin
keywords: amino acid and peptide transporters; cysteamine; intestinal health; pig