title: Characterization of Colorectal Cancer Development in Apc (min/+) Mice.
abstract: The Apc (min/+) mouse provides an excellent experimental model for studying genetic, environmental, and therapeutic aspects of intestinal neoplasia in humans. In this chapter, we will describe techniques for studying colon cancer development in Apc (min/+) mice on C57BL/6J (B6) background, focusing on the roles of environmental modifiers, including Dextran Sulfate Sodium (DSS), high fat diet, and bile acid supplementation in the context of experimental colorectal cancer. This chapter also includes protocols describing extraction and purification of DSS-contaminated RNA, as well as sampling, harvesting, and tissue processing. The common pathologic lesions encountered in these animals are described in detail.
authors.affiliation: Department of Pathology and Immunology, Washington University School of Medicine in St. Louis, 660 S. Euclid Ave, Box 8118, St. Louis, MO, 63110, USA. INalbantoglu@path.wustl.edu.
authors.forename: ILKe
authors.initials: I
authors.lastname: Nalbantoglu
authors.affiliation: Department of Medicine, Washington University School of Medicine in St. Louis, St. Louis, MO, USA.
authors.forename: Valerie
authors.initials: V
authors.lastname: Blanc
authors.affiliation: Department of Medicine, Washington University School of Medicine in St. Louis, St. Louis, MO, USA.
authors.forename: Nicholas O
authors.initials: NO
authors.lastname: Davidson
mesh_terms: D025601:Adenomatous Polyposis Coli Protein / Q000235:genetics*; D000818:Animals; D001647:Bile Acids and Salts / Q000633:toxicity; D015179:Colorectal Neoplasms / Q000139:chemically induced* / Q000235:genetics / Q000473:pathology*; D016264:Dextran Sulfate / Q000633:toxicity; D059305:Diet, High-Fat / Q000009:adverse effects; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D025601:Adenomatous Polyposis Coli Protein; D001647:Bile Acids and Salts; C000613192:adenomatous polyposis coli protein, mouse; D016264:Dextran Sulfate
keywords: Adenoma; Apc min/+; Bile acids; Colorectal cancer; Dextran sulfate sodium; High fat