title: RKIP expression of liver and kidney after arsenic exposure.
abstract: Arsenic is associated with cancers of kidney and liver. Raf kinase inhibitor protein (RKIP) has been identified as a member of a novel class of molecules that suppress the metastatic spread of tumors. In order to investigate the effect of arsenic to RKIP of liver and kidney, the expression of RKIP of liver and kidney with As (III) was explored in this study. Thirty male mice were chronically fed with 42.5 ppm, 85 ppm NaAsO2and water for 180 days. The kidney and liver accumulation levels of As (III) in mice were determined by electro-thermal atomic absorption spectrometry. The method of RT-PCR, Western blotting analysis and immunohistochemistry were used to determine gene expression and protein expression of RKIP. The results showed that arsenic level was significantly increased in kidney and liver of As (III)-exposed mice as compared with control group. The gene expression and protein expression of RKIP was significantly decreased in kidney and liver of As (III)-exposed mice in comparison with these of control mice. These data suggested that RKIP decrease of liver and kidney with As (III) may be dangerous index in formation of cancer. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 1079-1082, 2017.
authors.affiliation: Department of Medical Technology, Institute of Medical Technology, Fooyin University, Kaohsiung, Taiwan.
authors.forename: Der-An
authors.initials: DA
authors.lastname: Tsao
authors.affiliation: Department of Medical Technology, Institute of Medical Technology, Fooyin University, Kaohsiung, Taiwan.
authors.forename: Wei-Chang
authors.initials: WC
authors.lastname: Tseng
authors.affiliation: Department of Biomedical Engineering, I-Shou University, Kaohsiung, Taiwan.
authors.forename: Huoy-Rou
authors.initials: HR
authors.lastname: Chang
mesh_terms: D000818:Animals; D001151:Arsenic / Q000633:toxicity*; D020261:Arsenic Poisoning / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D015153:Blotting, Western; D005786:Gene Expression Regulation / Q000187:drug effects; D007150:Immunohistochemistry; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D049488:Phosphatidylethanolamine Binding Protein / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D049488:Phosphatidylethanolamine Binding Protein; C539308:Raf kinase inhibitory protein, mouse; D001151:Arsenic
keywords: Raf kinase inhibitor protein (RKIP); arsenic; kidney; liver