title: Quantitation of Plasma Membrane (G Protein-Coupled) Receptor Trafficking in Cultured Cells.
abstract: Measuring the functional behavior of G protein-coupled receptors (GPCRs) has been a major focus of academic and pharmaceutical research for many decades. These efforts have led to the development of many assays to measure the downstream effects of ligand binding on receptor activity. In this chapter, we describe an internalization/recycling assay that can be used to track changes in receptor number at the plasma membrane. Used in concert with other assays, this antibody-based technique can provide important information on GPCR activation by receptor-specific ligands.
authors.affiliation: Department of Psychological and Brain Sciences, Indiana University, Bloomington, IN, 47405, USA.
authors.forename: Jim
authors.initials: J
authors.lastname: Wager-Miller
authors.affiliation: Department of Psychological and Brain Sciences, Indiana University, Bloomington, IN, 47405, USA. kmackie@indiana.edu.
authors.forename: Ken
authors.initials: K
authors.lastname: Mackie
mesh_terms: D001681:Biological Assay / Q000379:methods*; D002460:Cell Line; D002478:Cells, Cultured; D015870:Gene Expression; D006801:Humans; D007700:Kinetics; D008024:Ligands; D011485:Protein Binding; D021381:Protein Transport; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000235:genetics / Q000378:metabolism*
chemical_list: D008024:Ligands; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled
keywords: Agonist; Antagonist; CB1 cannabinoid receptor (CB1); Epitope tag; G protein-coupled receptor (GPCR); G protein-coupled receptor kinase (GRK); Recycling