title: Preparation of Functional, Fluorescently Labeled mRNA Capped with Anthraniloyl-m(7)GpppG.
abstract: Fluorescent mRNA molecules offer a wide range of applications for studying capping/decapping reactions, translation, and other biophysical studies. Furthermore, fluorescent tags prove invaluable for tracking RNA molecules in cells. Here, we describe an efficient synthesis of a fluorescent cap analog, anthranioyl-GTP, its purification, and in vitro cap labeling of transcribed mRNA catalyzed by the recombinant vaccinia capping enzyme to produce anthranioyl-m(7)GpppG-capped RNA.
authors.affiliation: Department of Sciences, John Jay College of Criminal Justice, City University of New York, New York, NY, USA. adomashevskiy@jjay.cuny.edu.
authors.forename: Artem V
authors.initials: AV
authors.lastname: Domashevskiy
authors.affiliation: Department of Sciences, John Jay College of Criminal Justice, City University of New York, New York, NY, USA.
authors.forename: David J
authors.initials: DJ
authors.lastname: Rodriguez
authors.affiliation: Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Parkville, Victoria, Australia.|Department of Botany, University of Sri Jayewardenepura, Nugegoda, Sri Lanka.
authors.forename: Dilantha
authors.initials: D
authors.lastname: Gunawardana
authors.affiliation: Department of Chemistry, Hunter College, City University of New York, New York, NY, USA.
authors.forename: Dixie J
authors.initials: DJ
authors.lastname: Goss
mesh_terms: D006151:Guanosine / Q000031:analogs & derivatives / Q000737:chemistry; D015394:Molecular Structure; D014176:Protein Biosynthesis; D012314:RNA Cap Analogs / Q000138:chemical synthesis* / Q000737:chemistry; D012333:RNA, Messenger / Q000737:chemistry* / Q000235:genetics; D013050:Spectrometry, Fluorescence; D014158:Transcription, Genetic
chemical_list: D012314:RNA Cap Analogs; D012333:RNA, Messenger; D006151:Guanosine; C016578:7-methylguanosine
keywords: Ant-GTP; Anthranioyl-m7G; Capped RNA; Chromatography; Fluorescence; Fluorescently labeled RNA; RNA labeling