title: Effect of supplemented sericin on the development, cell number, cryosurvival and number of lipid droplets in cultured bovine embryos.
abstract: Sericin was investigated as an alternative to fetal bovine serum (FBS) for bovine embryo culture. In vitro matured oocytes were developed using 0.05%, 0.1% or 0.15% sericin. The developmental rate, cryosurvival rate and blastulation time of these embryos were compared with those of embryos developed using 5% FBS. The number of lipid droplets was compared among the blastocysts developed using 5% FBS, using 0.05% sericin and in vivo. The rate of cleavage and blastocyst formation was similar among all groups. Blastulation occurred significantly earlier in the embryos developed using 5% FBS than in those developed using sericin at any concentration (P < 0.05). At 72 h after thawing, the cryosurvival rate of the blastocysts developed using 5% FBS and 0.05% sericin were significantly higher compared with those developed using 0.1% and 0.15% sericin (P < 0.05). The blastocysts developed using 0.05% sericin and in vivo produced a significantly fewer number of medium and large lipid droplets than those developed using 5% FBS. These results suggest that the blastocysts developed using 0.05% sericin show characteristics similar to those of the blastocysts developed in vivo and that the use of sericin as an alternative to FBS is feasible.
authors.affiliation: National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan.
authors.forename: Misa
authors.initials: M
authors.lastname: Hosoe
authors.affiliation: National Livestock Breeding Center, Kato, Hokkaido, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Inaba
authors.affiliation: National Livestock Breeding Center, Nishigo, Fukushima, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Hashiyada
authors.affiliation: Department of Sustainable Agriculture, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Imai
authors.affiliation: National Institute of Livestock and Glassland Science, Tsukuba, Ibaraki, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Kajitani
authors.affiliation: National Institute of Livestock and Glassland Science, Tsukuba, Ibaraki, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Hasegawa
authors.affiliation: National Institute of Livestock and Glassland Science, Tsukuba, Ibaraki, Japan.
authors.forename: Mamoru
authors.initials: M
authors.lastname: Irie
authors.affiliation: National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan.
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Teramoto
authors.affiliation: Faculty of Agriculture, Iwate University, Morioka, Iwate, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Takahashi
authors.affiliation: Faculty of Agriculture, Niigata University, Niigata, Japan.
authors.forename: Sueo
authors.initials: S
authors.lastname: Niimura
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000378:metabolism; D002417:Cattle; D002452:Cell Count; D002470:Cell Survival / Q000187:drug effects*; D015925:Cryopreservation; D047108:Embryonic Development / Q000187:drug effects*; D066298:In Vitro Techniques; D066292:Lipid Droplets / Q000378:metabolism*; D009865:Oocytes / Q000254:growth & development* / Q000378:metabolism; D047030:Sericins / Q000494:pharmacology*; D044967:Serum
chemical_list: D047030:Sericins
keywords: cryosurvival; lipid droplets; sericin