title: Cryopreservation on a cryo-plate of Arundina graminifolia protocorms, dehydrated with silica gel and drying beads.
abstract: BACKGROUND
  There are various methods for the cryopreservation of plant material, with each biological specimen potentially requiring protocol optimization to maximize success.
  
  
  OBJECTIVE
  The aim of this study is to compare droplet-vitrification, encapsulation-dehydration, and the cryo-plate method for cryopreservation of protocorms of the orchid Arundina graminifolia, using silica gel and drying beads as the desiccation materials.
  
  
  METHODS
  The cryo-plate method included preculture of protocorms, developed from seeds, placed on aluminium cryo-plates and embedded in alginate gel. Cryo-plates were surface dried using sterile filter paper, placed in Petri dishes containing 50 g silica gel or 30 g drying beads in a laminar air-flow cabinet. Specimens on cryo-plates were dehydrated to 25 % moisture content, placed into 2 mL cryotubes and plunged directly into liquid nitrogen for 1 d.
  
  
  RESULTS
  For cryopreservation, the cryo-plate method, involving dehydration with 30 g drying beads gave the highest regrowth (77 %), followed by the encapsulation-dehydration method with 30 g drying beads (64 % regrowth) and the droplet-vitrification method, following exposure to PVS2 solution for 20 min (33 % regrowth).
  
  
  CONCLUSIONS
  Regrowth of cryopreserved protocorms using the cryo-plate method was rapid with the highest survival and regrowth.
authors.affiliation: Department of Plant Science, Faculty of Science, Mahidol University, Phayathai, Bangkok, Thailand.
authors.forename: L B
authors.initials: LB
authors.lastname: Cordova
authors.affiliation: Department of Plant Science, Faculty of Science, Mahidol University, Phayathai, Bangkok, Thailand. kanchitthammasiri@gmail.com.
authors.forename: K
authors.initials: K
authors.lastname: Thammasiri
mesh_terms: D015925:Cryopreservation / Q000295:instrumentation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D003890:Desiccation / Q000295:instrumentation; D029595:Orchidaceae / Q000502:physiology*; D012639:Seeds; D058428:Silica Gel / Q000494:pharmacology
chemical_list: D003451:Cryoprotective Agents; D058428:Silica Gel