title: Cadmium inhibits mouse sperm motility through inducing tyrosine phosphorylation in a specific subset of proteins.
abstract: Cadmium (Cd) has been reported to impair male fertility, primarily by disrupting sperm motility, but the underlying molecular mechanism remains unclear. Here we investigated the effects of Cd on sperm motility, tyrosine phosphorylation, AMP-activated protein kinase (AMPK) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity, and ATP levels in vitro. Our results demonstrated that Cd inhibited sperm motility, GAPDH activity, AMPK activity and ATP production, and induced tyrosine phosphorylation of 55-57KDa proteins. Importantly, all the parameters affected by Cd were restored to normal levels when incubated with 10μM Cd in the presence of 30μM ethylene diamine tetraacetic acid (EDTA). Interestingly, changes of tyrosine phosphorylation levels of 55-57KDa proteins are completely contrary to that of other parameters. These results suggest that Cd-induced tyrosine phosphorylation of 55-57KDa proteins might act as an engine to block intracellular energy metabolism and thus decrease sperm motility.
authors.affiliation: Shanghai Key Lab of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China.
authors.forename: Lirui
authors.initials: L
authors.lastname: Wang
authors.affiliation: Shanghai Key Lab of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Li
authors.affiliation: Shanghai Key Lab of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China.
authors.forename: Jieli
authors.initials: J
authors.lastname: Fu
authors.affiliation: Shanghai Key Lab of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China.
authors.forename: Linqing
authors.initials: L
authors.lastname: Zhen
authors.affiliation: Shanghai Key Lab of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China.
authors.forename: Na
authors.initials: N
authors.lastname: Zhao
authors.affiliation: Shanghai Key Lab of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China.
authors.forename: Qiangzhen
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Shanghai Key Lab of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China.
authors.forename: Sisi
authors.initials: S
authors.lastname: Li
authors.affiliation: Shanghai Key Lab of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China. Electronic address: lixinhong7172@sjtu.edu.cn.
authors.forename: Xinhong
authors.initials: X
authors.lastname: Li
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) / Q000378:metabolism; D008297:Male; D051379:Mice; D010766:Phosphorylation / Q000187:drug effects; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D014443:Tyrosine / Q000378:metabolism*
chemical_list: D002104:Cadmium; D014443:Tyrosine; D000255:Adenosine Triphosphate; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+); D055372:AMP-Activated Protein Kinases
keywords: AMPK; Cadmium; EDTA; GAPDH; Mouse sperm; Protein tyrosine phosphorylation