title: Chikungunya Virus Growth and Fluorescent Labeling: Detection of Chikungunya Virus by Immunofluorescence Assay.
abstract: Immunofluorescence assay (IFA) is a highly versatile and sensitive assay for detection and titration of chikungunya virus (CHIKV). The IFA technique requires virus-infected cells (viral antigen) and antibodies specific to the viral antigens for detection. Suitable antibodies for detection include monoclonal antibodies specific to CHIKV structural and nonstructural proteins, polyclonal antibodies, and convalescent serum samples. Here, the details of virus antigen preparation, detection by IFA method, and applications are described. The described IFA method is potentially useful in a wide range of studies including virus growth kinetics and virus infection mechanism studies. Additionally, the described IFA method can be modified for applications in arbovirus diagnosis, including CHIKV.
authors.affiliation: Department of Virology 1, National Institute of Infectious Diseases, Tokyo, Japan. sherry@nagasaki-u.ac.jp.|Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan. sherry@nagasaki-u.ac.jp.
authors.forename: Meng Ling
authors.initials: ML
authors.lastname: Moi
authors.affiliation: Department of Virology 1, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Takasaki
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000378:metabolism; D000914:Antibodies, Viral / Q000378:metabolism*; D000956:Antigens, Viral / Q000276:immunology*; D002460:Cell Line; D065632:Chikungunya Fever / Q000175:diagnosis / Q000276:immunology; D002646:Chikungunya virus / Q000254:growth & development* / Q000276:immunology; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D006801:Humans; D008856:Microscopy, Fluorescence; D014709:Vero Cells
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D000956:Antigens, Viral
keywords: Chikungunya virus; Flow cytometry; Fluorescence microscopy; Immunofluorescence assay