title: Analysis of Histone Deacetylase 7 (HDAC7) Alternative Splicing and Its Role in Embryonic Stem Cell Differentiation Toward Smooth Muscle Lineage.
abstract: Histone deacetylases (HDACs) have a central role in the regulation of gene expression, which undergoes alternative splicing during embryonic stem cell (ES) cell differentiation. Alternative splicing gives rise to vast diversity over gene information, arousing public concerns in the last decade. In this chapter, we describe a strategy to detect HDAC7 alternative splicing and analyze its function on ES cell differentiation.
authors.affiliation: Cardiovascular Division, King's College London, 125 Coldharbour Lane, London, SE5 9NU, UK.
authors.forename: Junyao
authors.initials: J
authors.lastname: Yang
authors.affiliation: Centre for Experimental Medicine, Queen's University Belfast, Belfast, BT9 7BL, UK.
authors.forename: Andriana
authors.initials: A
authors.lastname: Margariti
authors.affiliation: Cardiovascular Division, King's College London, 125 Coldharbour Lane, London, SE5 9NU, UK. lingfang.zeng@kcl.ac.uk.
authors.forename: Lingfang
authors.initials: L
authors.lastname: Zeng
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D002454:Cell Differentiation; D019070:Cell Lineage; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D005786:Gene Expression Regulation; D057809:HEK293 Cells; D006655:Histone Deacetylases / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D051379:Mice; D000066450:Mouse Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: C543274:Hdac7 protein, mouse; D006655:Histone Deacetylases
keywords: Alternative splicing; HDAC7; Luciferase assay; Plasmid construction