title: Modeling lactose hydrolysis for efficiency and selectivity: Toward the preservation of sialyloligosaccharides in bovine colostrum whey permeate.
abstract: Enzymatic hydrolysis of lactose has been shown to improve the efficiency and selectivity of membrane-based separations toward the recovery of bioactive oligosaccharides. Achieving maximum lactose hydrolysis requires intrinsic process optimization for each specific substrate, but the effects of those processing conditions on the target oligosaccharides are not well understood. Response surface methodology was used to investigate the effects of pH (3.25-8.25), temperature (35-55°C), reaction time (6 to 58 min), and amount of enzyme (0.05-0.25%) on the efficiency of lactose hydrolysis by β-galactosidase and on the preservation of biologically important sialyloligosaccharides (3'-siallylactose, 6'-siallylactose, and 6'-sialyl-N-acetyllactosamine) naturally present in bovine colostrum whey permeate. A central composite rotatable design was used. In general, β-galactosidase activity was favored at pH values ranging from 3.25 to 5.75, with other operational parameters having a less pronounced effect. A pH of 4.5 allowed for the use of a shorter reaction time (19 min), lower temperature (40°C), and reduced amount of enzyme (0.1%), but complete hydrolysis at a higher pH (5.75) required greater values for these operational parameters. The total amount of sialyloligosaccharides was not significantly altered by the reaction parameters evaluated, suggesting specificity of β-galactosidase from Aspergillus oryzae toward lactose as well as the stability of the oligosaccharides at pH, temperature, and reaction time evaluated.
authors.affiliation: Department of Food Science and Technology, University of California, Davis, One Shields Avenue, Davis 95616; Foods for Health Institute, University of California, Davis, One Shields Avenue, Davis 95616.
authors.forename: Juliana M L N
authors.initials: JMLN
authors.lastname: de Moura Bell
authors.affiliation: Department of Food Science and Technology, Universidade Federal Fluminense, Niteroi, Rio de Janeiro, 24230340, Brazil.
authors.forename: Leticia F M C
authors.initials: LFMC
authors.lastname: Aquino
authors.affiliation: Department of Food Science and Technology, University of California, Davis, One Shields Avenue, Davis 95616.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Food Science and Technology, University of California, Davis, One Shields Avenue, Davis 95616.
authors.forename: Joshua L
authors.initials: JL
authors.lastname: Cohen
authors.affiliation: Department of Food Science and Technology, University of California, Davis, One Shields Avenue, Davis 95616.
authors.forename: Hyeyoung
authors.initials: H
authors.lastname: Lee
authors.affiliation: Department of Food Science and Technology, Universidade Federal Fluminense, Niteroi, Rio de Janeiro, 24230340, Brazil.
authors.forename: Vitor L
authors.initials: VL
authors.lastname: de Melo Silva
authors.affiliation: Protimiza Consulting and Training in Experimental Design and Process Optimization, Campinas, SP, 13000 000, Brazil.
authors.forename: Maria I
authors.initials: MI
authors.lastname: Rodrigues
authors.affiliation: Department of Food Science and Technology, University of California, Davis, One Shields Avenue, Davis 95616; Foods for Health Institute, University of California, Davis, One Shields Avenue, Davis 95616. Electronic address: dbarile@ucdavis.edu.
authors.forename: Daniela
authors.initials: D
authors.lastname: Barile
mesh_terms: D000818:Animals; D001236:Aspergillus oryzae / Q000201:enzymology; D002417:Cattle; D003126:Colostrum / Q000737:chemistry*; D004355:Drug Stability; D005260:Female; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007785:Lactose / Q000378:metabolism*; D009844:Oligosaccharides / Q000032:analysis*; D011247:Pregnancy; D013696:Temperature; D000067796:Whey / Q000737:chemistry*; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D009844:Oligosaccharides; C016735:sialooligosaccharides; D001616:beta-Galactosidase; D007785:Lactose
keywords: colostrum; lactose hydrolysis; sialyloligosaccharides