title: Preventive effects of procyanidin A2 on glucose homeostasis, pancreatic and duodenal homebox 1, and glucose transporter 2 gene expression disturbance induced by bisphenol A in male mice.
abstract: Procyanidins (PCs) as oligomeric compounds with antidiabetic properties formed from catechin and epicatechin molecules. Bisphenol A(BPA) is a common chemical material use in food and beverage packaging. The aim of this study was to explore the protective effects of procyanidin A2 (PCA2) against glucose homeostasis disturbance and gene expression of pancreatic and duodenal homebox 1 (Pdx1) as well as glucose transporter 2 (Glut2) induced by BPA in male mice. First tested these five concentrations of PCA2 (3 - 300 μM) alone and in combination with BPA(100 μg/L), on insulin secretion from isolated islets at in vitro condition. Next, examined the influence of BPA and PCA2 on islet apoptosis using flowcytometry. At in vivo condition, the BPA (100 μg/kg) and PCA2 (10 μmol/kg) administered for 20 days then, blood glucose and insulin, Pdx1 and, Glut2 genes expression, and oxidative stress markers examined. The results indicated that PCA2 strongly prevents islet cells apoptosis induced by BPA and, co-administration of PCA2 and BPA modified hyperglycemia. BPA reduced Pdx1 and Glut2 mRNA expression and antioxidant level in pancreas tissue, whereas PCA2 prevented from these effects. The findings from these studies suggest that use of PCA2 rich plants have preventive effects on hyperglycemia, and type 2 diabetes.
authors.affiliation: Department of Physiology, Health Research Institute, Diabetes Research Center, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Ahangarpour
authors.affiliation: Department of Physiology, Cellular and Molecular Research Center, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. afshari.g@ajums.ac.ir.
authors.forename: G
authors.initials: G
authors.lastname: Afshari
authors.affiliation: Physiology Research Center, Research Center for Infectious Diseases of Digestive System, Department of Physiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
authors.forename: S A
authors.initials: SA
authors.lastname: Mard
authors.affiliation: Cellular and Molecular Research Center, Department of Immunology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran;
authors.forename: A
authors.initials: A
authors.lastname: Khodadadi
authors.affiliation: Cellular and Molecular Research Center, Department of Anatomical Sciences, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Hashemitabar
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001559:Benzhydryl Compounds / Q000494:pharmacology*; D001786:Blood Glucose / Q000032:analysis*; D002392:Catechin / Q000494:pharmacology* / Q000627:therapeutic use; D002478:Cells, Cultured; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D051273:Glucose Transporter Type 2 / Q000235:genetics*; D018398:Homeodomain Proteins / Q000235:genetics*; D006706:Homeostasis / Q000187:drug effects; D006943:Hyperglycemia / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism; D007328:Insulin / Q000097:blood; D007515:Islets of Langerhans / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D051379:Mice; D010636:Phenols / Q000494:pharmacology*; D044945:Proanthocyanidins / Q000494:pharmacology* / Q000627:therapeutic use; D012333:RNA, Messenger / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D001786:Blood Glucose; D002392:Catechin; D051273:Glucose Transporter Type 2; D018398:Homeodomain Proteins; D007328:Insulin; D008315:Malondialdehyde; D010636:Phenols; D044945:Proanthocyanidins; D012333:RNA, Messenger; D015534:Trans-Activators; C495682:Slc2a2 protein, mouse; C084010:pancreatic and duodenal homeobox 1 protein; C542735:procyanidin A2; C006780:bisphenol A; D000098826:Bisphenol A Compounds