title: Metagenomic sequencing reveals the relationship between microbiota composition and quality of Chinese Rice Wine.
abstract: Chinese Rice Wine (CRW) is a common alcoholic beverage in China. To investigate the influence of microbial composition on the quality of CRW, high throughput sequencing was performed for 110 wine samples on bacterial 16S rRNA gene and fungal Internal Transcribed Spacer II (ITS2). Bioinformatic analyses demonstrated that the quality of yeast starter and final wine correlated with microbial taxonomic composition, which was exemplified by our finding that wine spoilage resulted from a high proportion of genus Lactobacillus. Subsequently, based on Lactobacillus abundance of an early stage, a model was constructed to predict final wine quality. In addition, three batches of 20 representative wine samples selected from a pool of 110 samples were further analyzed in metagenomics. The results revealed that wine spoilage was due to rapid growth of Lactobacillus brevis at the early stage of fermentation. Gene functional analysis indicated the importance of some pathways such as synthesis of biotin, malolactic fermentation and production of short-chain fatty acid. These results led to a conclusion that metabolisms of microbes influence the wine quality. Thus, nurturing of beneficial microbes and inhibition of undesired ones are both important for the mechanized brewery.
authors.affiliation: Zhejiang-California International Nanosystem Institute, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.|College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors.forename: Xutao
authors.initials: X
authors.lastname: Hong
authors.affiliation: Realbio Genomics Institute, Shanghai, 200050, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Disease, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, 310003, China.|Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University, Hangzhou, 310003, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.affiliation: Realbio Genomics Institute, Shanghai, 200050, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Wu
authors.affiliation: Zhejiang-California International Nanosystem Institute, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.
authors.forename: Haiqin
authors.initials: H
authors.lastname: Tan
authors.affiliation: National Engineering Research Center for Chinese Rice Wine, Zhejiang Guyuelongshan Shaoxing Rice Wine Co.Ltd., Shaoxing, Zhejiang 312000, China.
authors.forename: Guangfa
authors.initials: G
authors.lastname: Xie
authors.affiliation: Realbio Genomics Institute, Shanghai, 200050, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Xu
authors.affiliation: National Engineering Research Center for Chinese Rice Wine, Zhejiang Guyuelongshan Shaoxing Rice Wine Co.Ltd., Shaoxing, Zhejiang 312000, China.
authors.forename: Huijun
authors.initials: H
authors.lastname: Zou
authors.affiliation: Zhejiang-California International Nanosystem Institute, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Yu
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Disease, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, 310003, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Disease, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, 310003, China.|Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University, Hangzhou, 310003, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Qin
mesh_terms: D005516:Food Microbiology; D007778:Lactobacillus / Q000235:genetics*; D054892:Metagenome; D064307:Microbiota / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014920:Wine / Q000382:microbiology*