title: Metabolomics Reveals Altered Lipid Metabolism in a Mouse Model of Endometriosis.
abstract: Endometriosis is a common chronic estrogen-dependent gynecological disease affecting 10% of women in their reproductive age. It is characterized by proliferation of functional endometrial glands and stroma outside the uterine cavity. In the present study, we used mass spectrometry-based lipidomics to investigate the alterations in serum lipid profiles of mice induced with endometriosis. We identified several dysregulated lipids such as phosphatidylcholines, sphingomyelins, phosphatidylethanolamines, and triglycerides and show that triglycerides may be due to a general inflammatory condition in the peritoneum. We also show that in addition to phosphatidylcholine alteration, there is also an effect in the ratio of phosphatidylcholine/phosphatidylethanolamine in serum of mice induced with the disease and that this change may be due to increased expression of the phosphatidylethanolamine N-methyltransferase gene. The study provides new insight into the etiology of endometriosis.
authors.affiliation: School of Medical Science and Technology, Indian Institute of Technology Kharagpur , Kharagpur, West Bengal 721302, India.
authors.forename: Mainak
authors.initials: M
authors.lastname: Dutta
authors.forename: Mallappa
authors.initials: M
authors.lastname: Anitha
authors.forename: Philip B
authors.initials: PB
authors.lastname: Smith
authors.forename: Christopher R
authors.initials: CR
authors.lastname: Chiaro
authors.affiliation: School of Biotechnology, Jawaharlal Nehru University , New Delhi 110067, India.
authors.forename: Meenu
authors.initials: M
authors.lastname: Maan
authors.affiliation: School of Medical Science and Technology, Indian Institute of Technology Kharagpur , Kharagpur, West Bengal 721302, India.
authors.forename: Koel
authors.initials: K
authors.lastname: Chaudhury
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Patterson
mesh_terms: D000818:Animals; D004715:Endometriosis / Q000209:etiology / Q000378:metabolism*; D005260:Female; D050356:Lipid Metabolism; D055432:Metabolomics; D051379:Mice; D010713:Phosphatidylcholines / Q000097:blood / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000097:blood / Q000378:metabolism; D013109:Sphingomyelins / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
chemical_list: D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D013109:Sphingomyelins; D014280:Triglycerides
keywords: endometriosis; metabolomics; phosphatidylcholine; phosphatidylethanolamine