title: Trace determination of safranin O dye using ultrasound assisted dispersive solid-phase micro extraction: Artificial neural network-genetic algorithm and response surface methodology.
abstract: In this study, ultrasound assisted dispersive solid-phase micro extraction combined with spectrophotometry (USA-DSPME-UV) method based on activated carbon modified with Fe2O3 nanoparticles (Fe2O3-NPs-AC) was developed for pre-concentration and determination of safranin O (SO). It is known that the efficiency of USA-DSPME-UV method may be affected by pH, amount of adsorbent, ultrasound time and eluent volume and the extent and magnitude of their contribution on response (in term of main and interaction part) was studied by using central composite design (CCD) and artificial neural network-genetic algorithms (ANN-GA). Accordingly by adjustment of experimental conditions suggested by ANN-GA at pH 6.5, 1.1mg of adsorbent, 10min ultrasound and 150μL of eluent volume led to achievement of best operation performance like low LOD (6.3ngmL(-1)) and LOQ (17.5ngmL(-1)) in the range of 25-3500ngmL(-1). In following stage, the SO content in real water and wastewater samples with recoveries between 93.27-99.41% with RSD lower than 3% was successfully determined.
authors.affiliation: Chemistry Department, Yasouj University, Yasouj 75918-74831, Iran.
authors.forename: Ebrahim Alipanahpour
authors.initials: EA
authors.lastname: Dil
authors.affiliation: Chemistry Department, Yasouj University, Yasouj 75918-74831, Iran. Electronic address: m_ghaedi@mail.yu.ac.ir.
authors.forename: Mehrorang
authors.initials: M
authors.lastname: Ghaedi
authors.affiliation: Chemistry Department, Yasouj University, Yasouj 75918-74831, Iran.
authors.forename: Arash
authors.initials: A
authors.lastname: Asfaram
authors.affiliation: Chemistry Department, Gachsaran Branch, Islamic Azad University, Gachsaran, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Mehrabi
authors.affiliation: Chemistry Department, Yasouj University, Yasouj 75918-74831, Iran.
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Bazrafshan
authors.affiliation: Chemistry Department, Gachsaran Branch, Islamic Azad University, Gachsaran, Iran.
authors.forename: Abdol Mohammad
authors.initials: AM
authors.lastname: Ghaedi
keywords: Artificial neural network; Central composite design; Genetic algorithms; Safranin O; Ultrasound assisted dispersive solid-phase micro extraction