title: The expression of prostaglandin-E2 and its receptor in the oviduct of Chinese brown frog (Rana dybowskii).
abstract: The Chinese brown frog (Rana dybowskii) has one special physiological phenomenon, which is that its oviduct expands prior to hibernation rather than in the breeding period. In this study, we investigated the immunolocalization and expression levels of prostaglandin-E2 (PGE2), cyclooxygenase (COX)-1 and COX-2, as well as one of its receptor subtypes 4 (EP4) in the oviduct of Rana dybowskii during the pre-hibernation and breeding period. PGE2, COX-1, COX-2 and EP4 have been observed in glandular and epithelial cells in the breeding period, whereas only in the epithelial cells during the pre-hibernation. Consistently, the protein levels of COX-2 and EP4 were higher in the pre-hibernation as compared to the breeding period, but the diversity of COX-1 was not obvious. In addition, oviductal PGE2 concentration was also significantly higher in the pre-hibernation. These results suggested that prostaglandin-E2 may play an important autocrine or paracrine role in oviductal cell proliferation and differentiation of Rana dybowskii during pre-hibernation.
authors.affiliation: College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, PR China.
authors.forename: Ruiqi
authors.initials: R
authors.lastname: Hu
authors.affiliation: College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, PR China.
authors.forename: Liqin
authors.initials: L
authors.lastname: Xi
authors.affiliation: College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Cao
authors.affiliation: College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Yang
authors.affiliation: College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, PR China.
authors.forename: Yuning
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Sheng
authors.affiliation: College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, PR China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Han
authors.affiliation: College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, PR China.
authors.forename: Zhengrong
authors.initials: Z
authors.lastname: Yuan
authors.affiliation: College of Basic Medicine, Changchun University of Traditional Chinese Medicine, Changchun 130117, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Guo
authors.affiliation: College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, PR China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Weng
authors.affiliation: College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, PR China. Electronic address: xumeiyu@bjfu.edu.cn.
authors.forename: Meiyu
authors.initials: M
authors.lastname: Xu
mesh_terms: D000818:Animals; D019898:Autocrine Communication / Q000235:genetics; D051545:Cyclooxygenase 1 / Q000235:genetics* / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000235:genetics* / Q000378:metabolism; D015232:Dinoprostone / Q000235:genetics* / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D006605:Hibernation / Q000235:genetics; D006801:Humans; D010057:Oviducts / Q000378:metabolism; D011898:Ranidae / Q000235:genetics / Q000378:metabolism; D058309:Receptors, Prostaglandin E, EP4 Subtype / Q000235:genetics* / Q000378:metabolism
chemical_list: D058309:Receptors, Prostaglandin E, EP4 Subtype; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D015232:Dinoprostone
keywords: COX-1; COX-2; EP4; Oviduct; PGE2; Rana dybowskii