title: Expression of Sam68 Associates with Neuronal Apoptosis and Reactive Astrocytes After Spinal Cord Injury.
abstract: Src-associated in mitosis (Sam68; 68 kDa) is a novel RNA-binding protein that belongs to the signal transduction and activation of RNA family involved in various biological processes. However, the expression and roles of Sam68 in the central nervous system remain unknown. In the present study, we performed a spinal cord injury (SCI) model in adult rats and found a significant increase of Sam68 protein levels in this model, which reached a peak at day 3 and then gradually returned to normal levels at day 14 after SCI. We use immunohistochemistry analysis revealing a widespread distribution of Sam68 in the spinal cord. In addition, double-immunofluorescence staining showed that Sam68 immunoreactivity was found predominantly in neurons and astrocytes. Moreover, colocalization of Sam68/active caspase-3 has been respectively detected in neuronal nuclei, and colocalization of Sam68/PCNA has been detected in glial fibrillary acidic protein. In vitro, we found that depletion of Sam68 by short interfering RNA inhibits neuronal apoptosis and astrocyte proliferation and decreases cyclin D1 protein levels. In conclusion, this is the first study to find the Sam68 expression in SCI. Our results suggest that Sam68 might be illustrated in the apoptosis of neurons and proliferation of astrocytes after SCI. This research will provide new drug targets for clinical treatment of SCI.
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Nantong University, Jiangsu Province Key Laboratory for Information and Molecular Drug Target, Nantong University, 20. Xisi Road, Nantong, 226001, Jiangsu, China.
authors.forename: Xinlei
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Orthopaedics, People's Hospital of Yixing City, Yixing, 214200, Jiangsu, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Nantong University, Jiangsu Province Key Laboratory for Information and Molecular Drug Target, Nantong University, 20. Xisi Road, Nantong, 226001, Jiangsu, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Qian
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Nantong University, Jiangsu Province Key Laboratory for Information and Molecular Drug Target, Nantong University, 20. Xisi Road, Nantong, 226001, Jiangsu, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Nantong University, Jiangsu Province Key Laboratory for Information and Molecular Drug Target, Nantong University, 20. Xisi Road, Nantong, 226001, Jiangsu, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Department of Orthopaedics, People's Hospital of Yixing City, Yixing, 214200, Jiangsu, China.
authors.forename: Zhenhuan
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Department of Rehabilitation, Nantong Second People's Hospital, Nantong, 226001, Jiangsu, China.
authors.forename: Xinjian
authors.initials: X
authors.lastname: Song
authors.affiliation: Department of Rehabilitation, Nantong Second People's Hospital, Nantong, 226001, Jiangsu, China.
authors.forename: Jianbing
authors.initials: J
authors.lastname: Ren
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Nantong University, Jiangsu Province Key Laboratory for Information and Molecular Drug Target, Nantong University, 20. Xisi Road, Nantong, 226001, Jiangsu, China. zhangfengdoctor@sina.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis; D001253:Astrocytes / Q000378:metabolism / Q000473:pathology; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D002453:Cell Cycle / Q000235:genetics; D049109:Cell Proliferation; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D006614:Hindlimb / Q000473:pathology / Q000503:physiopathology; D007150:Immunohistochemistry; D008297:Male; D009043:Motor Activity; D009474:Neurons / Q000378:metabolism* / Q000473:pathology*; D010641:Phenotype; D034741:RNA, Small Interfering / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function; D013119:Spinal Cord Injuries / Q000235:genetics* / Q000473:pathology* / Q000503:physiopathology
chemical_list: D015415:Biomarkers; D004268:DNA-Binding Proteins; C000615624:Khdrbs1 protein, rat; D034741:RNA, Small Interfering
keywords: Cell cycle; Neuronal apoptosis; Proliferation; Rat; Sam68; Spinal cord injury