title: Effect of Different Broad Waveband Lights on Membrane Lipids of a Cyanobacterium, Synechococcus sp., as Determined by UPLC-QToF-MS and Vibrational Spectroscopy.
abstract: Differential profile of membrane lipids and pigments of a Synechococcus sp. cyanobacterial strain cells exposed to blue, green, red and white light are determined by means of liquid chromatography and mass spectrometry or diode array detection. Raman and ATR-IR spectra of intact cells under the diverse light wavebands are also reported. Blue light cells exhibited an increased content of photosynthetic pigments as well as specific species of membrane glycerolipids as compared to cells exposed to other wavebands. The A630/A680 ratio indicated an increased content of phycobilisomes (PBS) in the blue light-exposed cells. Some differences in the protein conformation between the four light waveband-exposed cells were deduced from the variable absorbance at specific wavenumbers in the FT-Raman and ATR-FTIR spectra, in particular bands assigned to amide I and amide II. Bands from 1180 to 950 cm(-1) in the ATR-FTIR spectrum suggest degraded outer membrane polysaccharide in the blue light-exposed cells.
authors.affiliation: Centre for Biotechnology Development (CDB), Spanish Council for Scientific Research (CSIC), Francisco Vallés 8, Valladolid 47151, Spain. olimpio.montero@dicyl.csic.es.
authors.forename: Olimpio
authors.initials: O
authors.lastname: Montero
authors.affiliation: Centre for Biotechnology Development (CDB), Spanish Council for Scientific Research (CSIC), Francisco Vallés 8, Valladolid 47151, Spain. m.velasco@dicyl.csic.es.
authors.forename: Marta
authors.initials: M
authors.lastname: Velasco
authors.affiliation: Fisica de la Materia Condensada Department, University of Valladolid, Valladolid 47011, Spain. jausanz@gmail.com.
authors.forename: Aurelio
authors.initials: A
authors.lastname: Sanz-Arranz
authors.affiliation: Fisica de la Materia Condensada Department, University of Valladolid, Valladolid 47011, Spain. rull@fmc.uva.es.
authors.forename: Fernando
authors.initials: F
authors.lastname: Rull
keywords: IR; Raman; Synechococcus sp.; UPLC-MS; light quality; lipids; pigments; vibrational spectroscopy