title: Copper and lead removal from aqueous solutions by bacterial consortia acting as biosorbents.
abstract: A bacterial consortium was selected in the presence of Cu from sediment samples taken from Sepetiba Bay, Brazil, which is a site historically contaminated by metals. Bacteria were exposed to 0, 1, 6, 12.5, 25 and 50μg·mL(-1) Cu, Pb and Cu+Pb for 11days of bioassay. Results showed Alcanivorax dominance (81%) and cell counts of 10(8)cells·mL(-1). However, a reduction in dehydrogenase activity was observed from the fifth day of exposure for all Cu, Pb, and Cu+Pb concentrations tested. Esterase activity tended to increase, indicating higher energy demand to complete the bacterial lifecycle. Pb concentrations in the filtered culture medium (0.2μm) were below the detection limit, indicating biosorption, whereas concentrations of Cu were close to the tested concentrations, indicative of efflux. Results suggest the need for biomarkers, such as esterase and dehydrogenase enzymatic activity, in the assessment of resistance and tolerance of communities previously exposed to stressors.
authors.affiliation: Programa de Biologia Marinha e Ambientes Costeiros, Instituto de Biologia, Universidade Federal Fluminense, Niterói, RJ 24020-150, Brazil. Electronic address: carolccwaite@hotmail.com.
authors.forename: Carolina Coelho da Costa
authors.initials: CCDC
authors.lastname: Waite
authors.affiliation: Programa de Biologia Marinha e Ambientes Costeiros, Instituto de Biologia, Universidade Federal Fluminense, Niterói, RJ 24020-150, Brazil. Electronic address: guilhermeoas@yahoo.com.br.
authors.forename: Guilherme Oliveira Andrade
authors.initials: GOA
authors.lastname: da Silva
authors.affiliation: Programa Dinâmica dos Oceanos e da Terra, Instituto de Geociências, Universidade Federal Fluminense, Niterói, RJ 24210-346, Brazil. Electronic address: jbitencourt@gmail.com.
authors.forename: José Augusto Pires
authors.initials: JAP
authors.lastname: Bitencourt
authors.affiliation: Programa de Biologia Marinha e Ambientes Costeiros, Instituto de Biologia, Universidade Federal Fluminense, Niterói, RJ 24020-150, Brazil. Electronic address: esabadini@gmail.com.
authors.forename: Elisamara
authors.initials: E
authors.lastname: Sabadini-Santos
authors.affiliation: Programa de Biologia Marinha e Ambientes Costeiros, Instituto de Biologia, Universidade Federal Fluminense, Niterói, RJ 24020-150, Brazil. Electronic address: miriancrapez@id.uff.br.
authors.forename: Mirian Araújo Carlos
authors.initials: MAC
authors.lastname: Crapez
mesh_terms: D001419:Bacteria; D001673:Biodegradation, Environmental; D001938:Brazil; D003300:Copper; D007854:Lead; D059013:Microbial Consortia
chemical_list: D007854:Lead; D003300:Copper
keywords: Alcanivorax; Dehydrogenases; Esterases; Sepetiba Bay