title: In Vivo Myeloperoxidase Imaging and Flow Cytometry Analysis of Intestinal Myeloid Cells.
abstract: Myeloperoxidase (MPO) imaging is a non-invasive method to detect cells that produce the enzyme MPO that is most abundant in neutrophils, macrophages, and inflammatory monocytes. While lacking specificity for any of these three cell types, MPO imaging can provide guidance for further flow cytometry-based analysis of tissues where these cell types reside. Isolation of leukocytes from the intestinal tract is an error-prone procedure. Here, we describe a protocol for intestinal leukocyte isolation that works reliable in our hands and allows for flow cytometry-based analysis, in particular of neutrophils.
authors.affiliation: Department of Hematology, Oncology and Stem Cell Transplantation, Freiburg University Medical Center, Albert Ludwigs University Freiburg, Freiburg, Germany.|Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, Freiburg, Germany.|Faculty of Biology, University of Freiburg, Freiburg, Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Hülsdünker
authors.affiliation: Department of Hematology, Oncology and Stem Cell Transplantation, Freiburg University Medical Center, Albert Ludwigs University Freiburg, Freiburg, Germany. robert.zeiser@uniklinik-freiburg.de.|Klinik für Innere Medizin I, Schwerpunkt Hämatologie, Onkologie und Stammzelltransplantation, Universitätsklinikum Freiburg, Hugstetter Str. 55, 79106, Freiburg, Germany. robert.zeiser@uniklinik-freiburg.de.
authors.forename: Robert
authors.initials: R
authors.lastname: Zeiser
mesh_terms: D000818:Animals; D005434:Flow Cytometry / Q000379:methods*; D007422:Intestines / Q000166:cytology / Q000201:enzymology*; D007962:Leukocytes / Q000201:enzymology; D051379:Mice; D008807:Mice, Inbred BALB C; D022423:Myeloid Cells / Q000201:enzymology; D009195:Peroxidase / Q000378:metabolism*
chemical_list: D009195:Peroxidase
keywords: Graft-versus-host disease; In vivo bioluminescence imaging; Leukocyte isolation; Myeloperoxidase; Neutrophil granulocytes