title: Testing the Paracrine Properties of Human Mesenchymal Stem Cells Using Conditioned Medium.
abstract: Mesenchymal stem cells (MSC) produce and secrete a great variety of cytokines and chemokines that play beneficial paracrine actions when MSC are used for tissue repair. The conditioned medium (CM) derived from MSC can be used both in vitro and in vivo to test specific paracrine effects or to screen putative paracrine/autocrine mediators by proteomics.In this chapter, we describe a straightforward method to prepare MSC-derived CM. Furthermore, we summarize some in vitro assays useful for testing the cytoprotective, angiogenic, and regenerative activity of CM. These assays are very helpful when studying the role of MSC in cardiac repair and regeneration.
authors.affiliation: Department of Molecular Medicine, Unit of Cardiology, University of Pavia, Pavia, Italy.|Department of Cardiothoracic and Vascular Sciences - Coronary Care Unit and Laboratory of Clinical and Experimental Cardiology, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.|Laboratory of Experimental Cardiology for Cell and Molecular Therapy, Department of Cardiology, Fondazione IRCCS Policlinico San Matteo, Viale Golgi 19, Pavia, 27100, Italy.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Danieli
authors.affiliation: Department of Molecular Medicine, Unit of Cardiology, University of Pavia, Pavia, Italy.|Department of Cardiothoracic and Vascular Sciences - Coronary Care Unit and Laboratory of Clinical and Experimental Cardiology, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.|Laboratory of Experimental Cardiology for Cell and Molecular Therapy, Department of Cardiology, Fondazione IRCCS Policlinico San Matteo, Viale Golgi 19, Pavia, 27100, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Malpasso
authors.affiliation: Department of Molecular Medicine, Unit of Cardiology, University of Pavia, Pavia, Italy.|Department of Cardiothoracic and Vascular Sciences - Coronary Care Unit and Laboratory of Clinical and Experimental Cardiology, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.|Laboratory of Experimental Cardiology for Cell and Molecular Therapy, Department of Cardiology, Fondazione IRCCS Policlinico San Matteo, Viale Golgi 19, Pavia, 27100, Italy.
authors.forename: Maria Chiara
authors.initials: MC
authors.lastname: Ciuffreda
authors.affiliation: Department of Molecular Medicine, Unit of Cardiology, University of Pavia, Pavia, Italy. m.gnecchi@unipv.it.|Department of Cardiothoracic and Vascular Sciences - Coronary Care Unit and Laboratory of Clinical and Experimental Cardiology, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy. m.gnecchi@unipv.it.|Laboratory of Experimental Cardiology for Cell and Molecular Therapy, Department of Cardiology, Fondazione IRCCS Policlinico San Matteo, Viale Golgi 19, Pavia, 27100, Italy. m.gnecchi@unipv.it.|Department of Medicine, University of Cape Town, Cape Town, South Africa. m.gnecchi@unipv.it.
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Gnecchi
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D018925:Chemokines / Q000378:metabolism; D015925:Cryopreservation; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000166:cytology*; D019899:Paracrine Communication; D012038:Regeneration
chemical_list: D018925:Chemokines; D017077:Culture Media, Conditioned; D016207:Cytokines
keywords: Angiogenesis; Cardiac regeneration; Cardioprotection; Conditioned medium; Hypoxia/reoxygenation; Mesenchymal stem cells; Paracrine effect; Soluble factors