title: Determination of chloramphenicol in biological matrices by solid-phase membrane micro-tip extraction and capillary electrophoresis.
abstract: Solid-phase membrane micro-tip extraction (SPMMTE) and capillary electrophoresis (CE) methods were developed and validated for analysis of chloramphenicol in human plasma and urine samples. Iron composite nanoparticles were prepared using green technology. CE was carried out using a silica capillary (60 cm × 50 μm i.d.), phosphate buffer (50 mm, 8.0 pH)-acetonitrile (95:5, v/v) as the background electrolyte, 10 kV voltage, 280 nm detection, 20 s injection time and 27 ± 1°C temperature. Frusemide was used as an internal standard. The values of migration time, electrophoretic mobility, electrophoretic velocity and theoretical plates of chloramphenicol were 12.254 min, 4.44 × 10, 7.41 × 10 and 11,227. The limits of detection and quantitation of chloramphenicol were 0.1 and 1.0 μg/mL. Recovery of chloramphenicol in the standard solution was 95%. Solid-phase membrane micro-tip extraction and capillary electrophoresis methods may be used to analyze chloramphenicol in human plasma and urine samples of any patient.
authors.affiliation: Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh, 11451, Kingdom of Saudi Arabia.
authors.forename: Afzal
authors.initials: A
authors.lastname: Hussain
authors.affiliation: Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh, 11451, Kingdom of Saudi Arabia.
authors.forename: Mohammed F
authors.initials: MF
authors.lastname: Alajmi
authors.affiliation: Department of Chemistry, Jamia Millia Islamia (Central University), New Delhi, 110025, India.
authors.forename: Imran
authors.initials: I
authors.lastname: Ali
mesh_terms: D002701:Chloramphenicol / Q000032:analysis* / Q000097:blood / Q000652:urine; D019075:Electrophoresis, Capillary / Q000379:methods*; D005665:Furosemide / Q000097:blood / Q000652:urine; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D008567:Membranes, Artificial; D015203:Reproducibility of Results; D052617:Solid Phase Microextraction / Q000379:methods*
chemical_list: D008567:Membranes, Artificial; D002701:Chloramphenicol; D005665:Furosemide
keywords: capillary electrophoresis; chloramphenicol; composite iron nano particles; frusemide; solid-phase membrane micro-tip extraction