title: Antibiofilm activity of sandblasted and laser-modified titanium against microorganisms isolated from peri-implantitis lesions.
abstract: Infections due to biofilm-producing microorganisms are one of the main causes for the failure of dental implants. Increasing efforts have been made in order to develop new strategies to prevent biofilm formation. In this study, the biofilm development on a newly designed laser-modified titanium implant surface was evaluated and compared to that on conventional sandblasted titanium used in implant dentistry. The amount of biofilm produced by Staphylococcus aureus, Pseudomonas aeruginosa and Porphyromonas gingivalis isolated from peri-implantitis was assessed by a semi-quantitative spectrophotometric method and by confocal laser scanning microscopy. Results showed a lower biofilm production on laser-modified surface compared to the sandblasted one. In particular, a significantly lower total volume of the biomass was observed on laser-modified surface, while no significant changes in live/dead bacteria percentages were noticed between materials. Modifying the topography of the conventional implant surface with laser ablation could represent a promising approach for inhibiting biofilm formation.
authors.affiliation: a Laboratory of Clinical Chemistry and Microbiology , I.R.C.C.S. Galeazzi Orthopedic Institute , Milan , Italy.|b Laboratory of Technical Sciences for Laboratory Medicine, Department of Biomedical Science for Health , University of Milan , Milan , Italy.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Drago
authors.affiliation: a Laboratory of Clinical Chemistry and Microbiology , I.R.C.C.S. Galeazzi Orthopedic Institute , Milan , Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Bortolin
authors.affiliation: a Laboratory of Clinical Chemistry and Microbiology , I.R.C.C.S. Galeazzi Orthopedic Institute , Milan , Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: De Vecchi
authors.affiliation: a Laboratory of Clinical Chemistry and Microbiology , I.R.C.C.S. Galeazzi Orthopedic Institute , Milan , Italy.
authors.forename: Serse
authors.initials: S
authors.lastname: Agrappi
authors.affiliation: c Research Centre in Oral Implantology, Department of Biomedical, Surgical and Dental Sciences , University of Milan , Milan , Italy.|d Dental Clinic, I.R.C.C.S. Galeazzi Orthopedic Institute , Milan , Italy.
authors.forename: Roberto L
authors.initials: RL
authors.lastname: Weinstein
authors.affiliation: e Department of Public Health, Microbiology and Virology , University of Milan , Milan , Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Mattina
authors.affiliation: c Research Centre in Oral Implantology, Department of Biomedical, Surgical and Dental Sciences , University of Milan , Milan , Italy.|d Dental Clinic, I.R.C.C.S. Galeazzi Orthopedic Institute , Milan , Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Francetti
mesh_terms: D018441:Biofilms; D015921:Dental Implants / Q000382:microbiology*; D006801:Humans; D007834:Lasers; D018613:Microscopy, Confocal; D057873:Peri-Implantitis / Q000382:microbiology* / Q000517:prevention & control; D016966:Porphyromonas gingivalis / Q000502:physiology; D016459:Prosthesis-Related Infections / Q000382:microbiology / Q000517:prevention & control; D011550:Pseudomonas aeruginosa / Q000502:physiology; D013211:Staphylococcus aureus / Q000502:physiology; D013280:Stomatitis / Q000382:microbiology* / Q000517:prevention & control; D014025:Titanium
chemical_list: D015921:Dental Implants; D014025:Titanium
keywords: Biofilm; Confocal laser scanning microscopy; Dental implants; Dental materials; Titanium oxide