title: Application of Quantum Dot-Molecularly Imprinted Polymer Core-Shell Particles Sensitized with Graphene for Optosensing of N(ε)-Carboxymethyllysine in Dairy Products.
abstract: Hydrophobic CdSe/ZnS quantum dots (QDs) coated with a molecularly imprinted polymer (MIP) sensitized with graphene (Gra-QDs@MIP) were prepared through a one-pot reverse microemulsion polymerization at room temperature. Gra-QDs@MIP was used as a molecular recognition element to construct a N(ε)-carboxymethyllysine (CML) optosensor. Graphene was used as a polymerization platform to increase the stability and kinetic binding properties of the system. Reverse microemulsion polymerization can anchor silica spheres on the surface of the QDs. This provides functional groups on the surface of Gra-QDs@MIP, which can bind CML and improve the fluorescence stability. Selective and sensitive optosensing of CML is possible at concentrations down to 3.0 μg L(-1) using Gra-QDs@MIP. Gra-QDs@MIP can be applied to dairy samples, as a recognition and response element for determining CML concentrations. The optosensing method was validated by high-performance liquid chromatography-mass spectrometry. The optosensor is economically and easily prepared, and the method is simple, fast, accurate, and reproducible.
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University (BTBU) , 11 Fucheng Road, Beijing 100048, People's Republic of China.
authors.forename: Huilin
authors.initials: H
authors.lastname: Liu
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University (BTBU) , 11 Fucheng Road, Beijing 100048, People's Republic of China.
authors.forename: Xiaomo
authors.initials: X
authors.lastname: Chen
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University (BTBU) , 11 Fucheng Road, Beijing 100048, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Mu
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University (BTBU) , 11 Fucheng Road, Beijing 100048, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University (BTBU) , 11 Fucheng Road, Beijing 100048, People's Republic of China.
authors.forename: Baoguo
authors.initials: B
authors.lastname: Sun
mesh_terms: D003611:Dairy Products / Q000032:analysis*; D005504:Food Analysis / Q000295:instrumentation / Q000379:methods*; D006108:Graphite; D057927:Hydrophobic and Hydrophilic Interactions; D041943:Infant Formula / Q000032:analysis / Q000737:chemistry; D008239:Lysine / Q000031:analogs & derivatives* / Q000032:analysis; D054802:Molecular Imprinting / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry / Q000378:metabolism; D055095:Optics and Photonics / Q000295:instrumentation / Q000379:methods; D045663:Quantum Dots / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
chemical_list: C048496:N(6)-carboxymethyllysine; D006108:Graphite; D008239:Lysine
keywords: Nε-carboxymethyllysine; dairy product; graphene; molecularly imprinted polymer; quantum dot