title: Quantitative comparison of cancer and normal cell adhesion using organosilane monolayer templates: an experimental study on the anti-adhesion effect of green-tea catechins.
abstract: The main constituent of green tea, (-)-Epigallocatechin-3-O-gallate (EGCG), is known to have cancer-specific chemopreventive effects. In the present work, we investigated how EGCG suppresses cell adhesion by comparing the adhesion of human pancreatic cancer cells (AsPC-1 and BxPC-3) and their counterpart, normal human embryonic pancreas-derived cells (1C3D3), in catechin-containing media using organosilane monolayer templates (OMTs). The purpose of this work is (1) to evaluate the quantitativeness in the measurement of cell adhesion with the OMT and (2) to show how green-tea catechins suppress cell adhesion in a cancer-specific manner. For the first purpose, the adhesion of cancer and normal cells was compared using the OMT. The cell adhesion in different type of catechins such as EGCG, (-)-Epicatechin-3-O-gallate (ECG) and (-)-Epicatechin (EC) was also evaluated. The measurements revealed that the anti-adhesion effect of green-tea catechins is cancer-specific, and the order is EGCG≫ECG>EC. The results agree well with the data reported to date, showing the quantitativeness of the new method. For the second purpose, the contact area of cells on the OMT was measured by reflection interference contrast microscopy. The cell-OMT contact area of cancer cells decreases with increasing EGCG concentration, whereas that of normal cells remains constant. The results reveal a twofold action of EGCG on cancer cell adhesion-suppressing cell attachment to a candidate adhesion site and decreasing the contact area of the cells-and validates the use of OMT as a tool for screening cancer cell adhesion.
authors.affiliation: School of Fundamental Science and Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku, Tokyo, 169-8555, Japan.
authors.forename: Rumi
authors.initials: R
authors.lastname: Sakamoto
authors.affiliation: School of Fundamental Science and Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku, Tokyo, 169-8555, Japan.
authors.forename: Eisuke
authors.initials: E
authors.lastname: Kakinuma
authors.affiliation: School of Fundamental Science and Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku, Tokyo, 169-8555, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Masuda
authors.affiliation: School of Fundamental Science and Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku, Tokyo, 169-8555, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Takeuchi
authors.affiliation: Depertment of Chemistry, Saitama University, 225 Shimo-ohkubo, Sakura-ku, Saitama, 338-8570, Japan.
authors.forename: Kosaku
authors.initials: K
authors.lastname: Ito
authors.affiliation: Depertment of Chemistry, Saitama University, 225 Shimo-ohkubo, Sakura-ku, Saitama, 338-8570, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Iketaki
authors.affiliation: Depertment of Chemistry, Saitama University, 225 Shimo-ohkubo, Sakura-ku, Saitama, 338-8570, Japan.
authors.forename: Takahisa
authors.initials: T
authors.lastname: Matsuzaki
authors.affiliation: Depertment of Chemistry, Saitama University, 225 Shimo-ohkubo, Sakura-ku, Saitama, 338-8570, Japan.
authors.forename: Seiichiro
authors.initials: S
authors.lastname: Nakabayashi
authors.affiliation: Depertment of Chemistry, Saitama University, 225 Shimo-ohkubo, Sakura-ku, Saitama, 338-8570, Japan.
authors.forename: Hiroshi Y
authors.initials: HY
authors.lastname: Yoshikawa
authors.affiliation: Research Organization for Nano & Life Innovation, Waseda University, 513 Waseda-tsurumakicho, Shinjuku, Tokyo, 162-0041, Japan.|Frontier Research Institute for Interdisciplinary Sciences, Tohoku University, 6-3 Aramakiaza-aoba, Aoba, Sendai, Miyagi, 980-8578, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Yamamoto
authors.affiliation: Research Organization for Nano & Life Innovation, Waseda University, 513 Waseda-tsurumakicho, Shinjuku, Tokyo, 162-0041, Japan.|The Japanese Red Cross College of Nursing, 4-1-3 Hiroo, Shibuya, Tokyo, 150-0012, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Sato
authors.affiliation: School of Fundamental Science and Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku, Tokyo, 169-8555, Japan. tanii@waseda.jp.|Research Organization for Nano & Life Innovation, Waseda University, 513 Waseda-tsurumakicho, Shinjuku, Tokyo, 162-0041, Japan. tanii@waseda.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Tanii
mesh_terms: D002392:Catechin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects*; D045744:Cell Line, Tumor; D006801:Humans; D010179:Pancreas / Q000187:drug effects*; D010190:Pancreatic Neoplasms / Q000188:drug therapy* / Q000473:pathology; D013662:Tea / Q000737:chemistry
chemical_list: D013662:Tea; D002392:Catechin; C045651:epigallocatechin gallate
keywords: Anti-adhesion effect; Cell adhesion; Green-tea catechin; Organosilane monolayer template; Reflection interference contrast microscopy