title: Developmental validation of forensic DNA-STR kits: Expressmarker 16+10Y and expressmarker 16+18Y.
abstract: DNA-STR analysis is widely used in the forensic science field and obtaining results in shorter time is highly demanded. The developed forensic STR Kit, referred to as Expressmarker 16+10Y (EX16+10Y) and Expressmarker 16+18Y (EX16+18Y), could amplify the common autosomal and Y chromosome STR loci simultaneously. The kits are validated by a series of tests, including DNA mixtures, stutter ratios, PCR based studies, species specificities, inhibitors, sensitivity, sizing precision, reproducibility and parallel tests. The results demonstrated that EX16+10Y and EX16+18Y were useful tools for rapid criminal investigation.
authors.affiliation: Institute of Forensic Science, Shanghai Municipal Public Security Bureau/Key Laboratory of Forensic Evidence and Scene Technology, Ministry of Public Security/Shanghai Key Laboratory of Crime Scene Evidence, Shanghai 200083, China. Electronic address: hgzhou803@hotmail.com.
authors.forename: Huaigu
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Institute of Forensic Science, Shanghai Municipal Public Security Bureau/Key Laboratory of Forensic Evidence and Scene Technology, Ministry of Public Security/Shanghai Key Laboratory of Crime Scene Evidence, Shanghai 200083, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Bi
authors.affiliation: Institute of Forensic Science, Shanghai Municipal Public Security Bureau/Key Laboratory of Forensic Evidence and Scene Technology, Ministry of Public Security/Shanghai Key Laboratory of Crime Scene Evidence, Shanghai 200083, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Institute of Forensic Science, Shanghai Municipal Public Security Bureau/Key Laboratory of Forensic Evidence and Scene Technology, Ministry of Public Security/Shanghai Key Laboratory of Crime Scene Evidence, Shanghai 200083, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Forensic Science, Shanghai Municipal Public Security Bureau/Key Laboratory of Forensic Evidence and Scene Technology, Ministry of Public Security/Shanghai Key Laboratory of Crime Scene Evidence, Shanghai 200083, China.
authors.forename: Ronghua
authors.initials: R
authors.lastname: Chen
authors.affiliation: AGCU ScienTech Incorporation, Wuxi 214174, China.
authors.forename: Fayuan
authors.initials: F
authors.lastname: Li
authors.affiliation: AGCU ScienTech Incorporation, Wuxi 214174, China.
authors.forename: Xinglin
authors.initials: X
authors.lastname: Mei
authors.affiliation: AGCU ScienTech Incorporation, Wuxi 214174, China.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Guo
authors.affiliation: AGCU ScienTech Incorporation, Wuxi 214174, China.
authors.forename: Weiguo
authors.initials: W
authors.lastname: Zheng
mesh_terms: D000818:Animals; D041322:Chromosomes, Human, Y; D016172:DNA Fingerprinting / Q000295:instrumentation*; D006801:Humans; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D013045:Species Specificity
keywords: EX16+10Y; EX16+18Y; Forensic STR kit