title: Abietane diterpenes from the cones of Abies numidica de Lannoy ex Carrière (Pinaceae) and in vitro evaluation of their antimicrobial properties.
abstract: Eight known abietane diterpenes (1-8) were isolated for the first time from Abies numidica cones (Pinaceae). The structures of all compounds were established by means of 1D and 2D NMR, and UV spectral analyses. The hydromethanolic extract of A. numidica cones was tested for its antimicrobial activity against 17 gram-positive and gram-negative bacteria and against five yeasts by the use of liquid and solid medium and bioautography methods. The best antimicrobial activity was found against gram-positive bacteria (MIC ≤ 0.3 mg/mL) Bacillus subtilis, Enterococcus faecalis ATCC 1034, Staphylococcus aureus 8325.4, S. aureus CIP 53.154, Micrococcus luteus and Listeria innocua and against Candida yeasts. The determination of MIC's of isolated products showed a high activity of compounds 4 and 6 against S. aureus, L. innocua (MIC = 62.5 μg/mL) and E. faecalis (MIC = 125 μg/mL).
authors.affiliation: a Département de chimie, Laboratoire d'Obtention de Substances Thérapeutiques (L.O.S.T) , Université des frères Mentouri-Constantine , Constantine , Algeria.
authors.forename: Maya
authors.initials: M
authors.lastname: Belhadj Mostefa
authors.affiliation: b Groupe Isolement et Structure, Institut de Chimie Moléculaire de Reims (ICMR), CNRS UMR 7312 , Reims , France.
authors.forename: Amin
authors.initials: A
authors.lastname: Abedini
authors.affiliation: b Groupe Isolement et Structure, Institut de Chimie Moléculaire de Reims (ICMR), CNRS UMR 7312 , Reims , France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Voutquenne-Nazabadioko
authors.affiliation: c Laboratoire de Microbiologie , UFR de Pharmacie , Reims , France.
authors.forename: Sophie C
authors.initials: SC
authors.lastname: Gangloff
authors.affiliation: a Département de chimie, Laboratoire d'Obtention de Substances Thérapeutiques (L.O.S.T) , Université des frères Mentouri-Constantine , Constantine , Algeria.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Kabouche
authors.affiliation: a Département de chimie, Laboratoire d'Obtention de Substances Thérapeutiques (L.O.S.T) , Université des frères Mentouri-Constantine , Constantine , Algeria.
authors.forename: Zahia
authors.initials: Z
authors.lastname: Kabouche
mesh_terms: D028202:Abies / Q000737:chemistry*; D045784:Abietanes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000494:pharmacology*; D013293:Enterococcus faecalis / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology*; D013211:Staphylococcus aureus
chemical_list: D045784:Abietanes; D000890:Anti-Infective Agents; D010936:Plant Extracts
keywords: Abies numidica; Pinaceae; abietane diterpenes; antimicrobial activity; bioautography; liquid medium; solid medium