title: A Chloroplast-Localized Rubredoxin Family Protein Gene from Puccinellia tenuiflora (PutRUB) Increases NaCl and NaHCO₃ Tolerance by Decreasing H₂O₂ Accumulation.
abstract: Rubredoxin is one of the simplest iron-sulfur (Fe-S) proteins. It is found primarily in strict anaerobic bacteria and acts as a mediator of electron transfer participation in different biochemical reactions. The PutRUB gene encoding a chloroplast-localized rubredoxin family protein was screened from a yeast full-length cDNA library of Puccinellia tenuiflora under NaCl and NaHCO₃ stress. We found that PutRUB expression was induced by abiotic stresses such as NaCl, NaHCO₃, CuCl₂ and H₂O₂. These findings suggested that PutRUB might be involved in plant responses to adversity. In order to study the function of this gene, we analyzed the phenotypic and physiological characteristics of PutRUB transgenic plants treated with NaCl and NaHCO₃. The results showed that PutRUB overexpression inhibited H₂O₂ accumulation, and enhanced transgenic plant adaptability to NaCl and NaHCO₃ stresses. This indicated PutRUB might be involved in maintaining normal electron transfer to reduce reactive oxygen species (ROS) accumulation.
authors.affiliation: Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education, Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin Hexing Road, Harbin 150040, China. ly7966@163.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education, Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin Hexing Road, Harbin 150040, China. belle.pp.liu@gmail.com.
authors.forename: Panpan
authors.initials: P
authors.lastname: Liu
authors.affiliation: Asian Natural Environmental Science Center, University of Tokyo, Nishitokyo-shi, Tokyo 188-0002, Japan. takano@anesc.u-tokyo.ac.jp.
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Takano
authors.affiliation: Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education, Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin Hexing Road, Harbin 150040, China. shenkuiliu@nefu.edu.cn.
authors.forename: Shenkui
authors.initials: S
authors.lastname: Liu
mesh_terms: D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D015723:Gene Library; D061125:Genes, Chloroplast; D005810:Multigene Family; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D006109:Poaceae / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D012416:Rubredoxins / Q000235:genetics* / Q000378:metabolism*; D017693:Sodium Bicarbonate / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological
chemical_list: D010940:Plant Proteins; D012416:Rubredoxins; D012965:Sodium Chloride; D017693:Sodium Bicarbonate
keywords: Puccinellia tenuiflora; ROS; rubredoxin family protein