title: [Abnormal expression of genes that regulate retinoid metabolism and signaling in non-small-cell lung cancer].
abstract: Retinoids are signaling molecules that control a wide variety of cellular processes and possess antitumor activity. This work presents a comprehensive description of changes in the expression of 23 genes that regulate retinoid metabolism and signaling in non-small-cell lung cancer tumors compared to adjacent normal tissues obtained using RT-PCR. Even at early stages of malignant transformation, a significant decrease in ADH1B, ADH3, RDHL, and RALDH1 mRNA levels was observed in 82, 79, 73, and 64% of tumor specimens, respectively, and a considerable increase in AKR1B10 mRNA content was observed in 80% of tumors. Dramatic changes in the levels of these mRNAs can impair the synthesis of all-trans retinoic acid, a key natural regulatory retinoid. Apart from that, it was found that mRNA levels of nuclear retinoid receptor genes RXRγ, RARα, RXRα, and gene RDH11 were significantly decreased in 80, 67, 57, and 66% of tumor specimens, respectively. Thus, neoplastic transformation of lung tissue cells is accompanied with deregulated expression of key genes of retinoid metabolism and function.
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
authors.forename: E S
authors.initials: ES
authors.lastname: Kuznetsova
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
authors.forename: O L
authors.initials: OL
authors.lastname: Zinovieva
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
authors.forename: N Yu
authors.initials: NY
authors.lastname: Oparina
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
authors.forename: M M
authors.initials: MM
authors.lastname: Prokofjeva
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
authors.forename: P V
authors.initials: PV
authors.lastname: Spirin
authors.affiliation: Blokhin Cancer Research Center, The Ministry of Health of the Russian Federation, Moscow, 115478, Russia.
authors.forename: I A
authors.initials: IA
authors.lastname: Favorskaya
authors.affiliation: Blokhin Cancer Research Center, The Ministry of Health of the Russian Federation, Moscow, 115478, Russia.
authors.forename: I B
authors.initials: IB
authors.lastname: Zborovskaya
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
authors.forename: N A
authors.initials: NA
authors.lastname: Lisitsyn
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
authors.forename: V S
authors.initials: VS
authors.lastname: Prassolov
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.|tamashkova@yandex.ru.
authors.forename: T D
authors.initials: TD
authors.lastname: Mashkova
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000096:biosynthesis* / Q000235:genetics; D000426:Alcohol Dehydrogenase / Q000096:biosynthesis* / Q000235:genetics; D000444:Aldehyde Dehydrogenase / Q000096:biosynthesis* / Q000235:genetics; D000080924:Aldehyde Dehydrogenase 1 Family; D000449:Aldehyde Reductase / Q000096:biosynthesis* / Q000235:genetics; D000074408:Aldo-Keto Reductases; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D012333:RNA, Messenger / Q000096:biosynthesis; D018168:Receptors, Retinoic Acid / Q000096:biosynthesis / Q000235:genetics; D050697:Retinal Dehydrogenase; D000072482:Retinoic Acid Receptor alpha; D012176:Retinoids / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D014212:Tretinoin / Q000378:metabolism
chemical_list: C000607071:RARA protein, human; D012333:RNA, Messenger; D018168:Receptors, Retinoic Acid; D000072482:Retinoic Acid Receptor alpha; D012176:Retinoids; D014212:Tretinoin; D015096:3-Hydroxysteroid Dehydrogenases; C432414:DHRS9 protein, human; C120471:AKR1B10 protein, human; D000074408:Aldo-Keto Reductases; C489332:ADH1B protein, human; C420272:ADH1C protein, human; D000426:Alcohol Dehydrogenase; D000449:Aldehyde Reductase; D000080924:Aldehyde Dehydrogenase 1 Family; D000444:Aldehyde Dehydrogenase; C510223:ALDH1A1 protein, human; D050697:Retinal Dehydrogenase
keywords: biosynthesis of retinoic acid; gene expression; nonsmall-cell lung cancer; retinoid metabolism; retinoid receptor genes