title: Antioxidant and Hypolipidemic Activity of the Hydroethanolic Extract of Curatella americana L. Leaves.
abstract: High levels of reactive oxygen species in the body and hyperlipidemia are key factors for the development of cardiovascular diseases such as atherosclerosis. The present study investigated the antioxidant and hypolipidemic activity of hydroethanolic extract of Curatella americana L. leaves (ExC). The antioxidant activity of ExC was assessed by 2,2-diphenyl-1-picrylhydrazyl free radical (DPPH) scavenging capacity and protection against hemolysis induced by 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH), followed by quantification of malondialdehyde (MDA). Wistar rats with hyperlipidemia induced by high-fructose diet (60%) were treated for 60 days with water, simvastatin (30 mg·Kg(-1)), ciprofibrate (2 mg·Kg(-1)), and ExC (200 mg·Kg(-1)). ExC revealed IC50 of 6.0 ± 0.5 μg·mL(-1), an intermediary value among positive controls used in the assay of DPPH scavenging capacity. At all concentrations (50 to 125 μg·mL(-1)) and times (60 to 240 min) evaluated, ExC protected erythrocytes against AAPH-induced hemolysis, which was confirmed by lower MDA levels. In vivo tests showed a reduction of 34 and 45%, respectively, in serum concentration of cholesterol and triglycerides in hyperlipidemic rats treated with ExC, a similar effect compared to the reference drugs, simvastatin and ciprofibrate, respectively. Together, the results showed the antioxidant activity of ExC and its ability to improve the serum lipid profile in hyperlipidemic rats.
authors.affiliation: School of Environmental and Biological Science, Federal University of Grande Dourados, Rodovia Dourados Ithaum, Km 12, 79804-970 Dourados, MS, Brazil.
authors.forename: Rafael Henrique Oliveira
authors.initials: RH
authors.lastname: Lopes
authors.affiliation: School of Environmental and Biological Science, Federal University of Grande Dourados, Rodovia Dourados Ithaum, Km 12, 79804-970 Dourados, MS, Brazil.
authors.forename: Luis Fernando Benitez
authors.initials: LF
authors.lastname: Macorini
authors.affiliation: School of Environmental and Biological Science, Federal University of Grande Dourados, Rodovia Dourados Ithaum, Km 12, 79804-970 Dourados, MS, Brazil.
authors.forename: Katia Ávila
authors.initials: KÁ
authors.lastname: Antunes
authors.affiliation: School of Environmental and Biological Science, Federal University of Grande Dourados, Rodovia Dourados Ithaum, Km 12, 79804-970 Dourados, MS, Brazil.
authors.forename: Priscilla Pereira de Toledo
authors.initials: PP
authors.lastname: Espindola
authors.affiliation: School of Environmental and Biological Science, Federal University of Grande Dourados, Rodovia Dourados Ithaum, Km 12, 79804-970 Dourados, MS, Brazil.
authors.forename: Tamaeh Monteiro
authors.initials: TM
authors.lastname: Alfredo
authors.affiliation: School of Environmental and Biological Science, Federal University of Grande Dourados, Rodovia Dourados Ithaum, Km 12, 79804-970 Dourados, MS, Brazil.
authors.forename: Paola Dos Santos
authors.initials: Pdos S
authors.lastname: da Rocha
authors.affiliation: School of Environmental and Biological Science, Federal University of Grande Dourados, Rodovia Dourados Ithaum, Km 12, 79804-970 Dourados, MS, Brazil.
authors.forename: Zefa Valdivina
authors.initials: ZV
authors.lastname: Pereira
authors.affiliation: School of Environmental and Biological Science, Federal University of Grande Dourados, Rodovia Dourados Ithaum, Km 12, 79804-970 Dourados, MS, Brazil.
authors.forename: Edson Lucas
authors.initials: EL
authors.lastname: Dos Santos
authors.affiliation: School of Environmental and Biological Science, Federal University of Grande Dourados, Rodovia Dourados Ithaum, Km 12, 79804-970 Dourados, MS, Brazil.
authors.forename: Kely
authors.initials: K
authors.lastname: de Picoli Souza
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology; D000070601:Diet, Carbohydrate Loading; D045466:Dilleniaceae / Q000737:chemistry* / Q000378:metabolism; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects; D006949:Hyperlipidemias / Q000188:drug therapy / Q000209:etiology; D000960:Hypolipidemic Agents / Q000737:chemistry* / Q000627:therapeutic use; D015227:Lipid Peroxidation / Q000187:drug effects; D008055:Lipids / Q000097:blood; D008315:Malondialdehyde / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D019821:Simvastatin / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D000960:Hypolipidemic Agents; D008055:Lipids; D010936:Plant Extracts; D008315:Malondialdehyde; D019821:Simvastatin