title: Backfilling-Free Strategy for Biopatterning on Intrinsically Dual-Functionalized Poly[2-Aminoethyl Methacrylate-co-Oligo(Ethylene Glycol) Methacrylate] Films.
abstract: We demonstrated protein and cellular patterning with a soft lithography technique using poly[2-aminoethyl methacrylate-co-oligo(ethylene glycol) methacrylate] films on gold surfaces without employing a backfilling process. The backfilling process plays an important role in successfully generating biopatterns; however, it has potential disadvantages in several interesting research and technical applications. To overcome the issue, a copolymer system having highly reactive functional groups and bioinert properties was introduced through a surface-initiated controlled radical polymerization with 2-aminoethyl methacrylate hydrochloride (AMA) and oligo(ethylene glycol) methacrylate (OEGMA). The prepared poly(AMA-co-OEGMA) film was fully characterized, and among the films having different thicknesses, the 35 nm-thick biotinylated, poly(AMA-co-OEGMA) film exhibited an optimum performance, such as the lowest nonspecific adsorption and the highest specific binding capability toward proteins.
authors.affiliation: Department of Chemistry and Center for Cell-Encapsulation Research, KAIST, Daejeon, 34141, South Korea.
authors.forename: Bong Soo
authors.initials: BS
authors.lastname: Lee
authors.affiliation: Department of Chemistry and Center for Cell-Encapsulation Research, KAIST, Daejeon, 34141, South Korea.
authors.forename: Juno
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Chemistry and Green-Nano Materials Research Center, Kyungpook National University, Daegu, 41566, South Korea.
authors.forename: Gyeongyeop
authors.initials: G
authors.lastname: Han
authors.affiliation: Department of Chemistry and Green-Nano Materials Research Center, Kyungpook National University, Daegu, 41566, South Korea.
authors.forename: EunRae
authors.initials: E
authors.lastname: Ha
authors.affiliation: Department of Chemistry and Center for Cell-Encapsulation Research, KAIST, Daejeon, 34141, South Korea.
authors.forename: Insung S
authors.initials: IS
authors.lastname: Choi
authors.affiliation: Department of Chemistry and Green-Nano Materials Research Center, Kyungpook National University, Daegu, 41566, South Korea. jkl@knu.ac.kr.
authors.forename: Jungkyu K
authors.initials: JK
authors.lastname: Lee
mesh_terms: D000327:Adsorption; D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D019855:Ethylene Glycol / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D008689:Methacrylates / Q000737:chemistry*; D051379:Mice; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D041681:NIH 3T3 Cells; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D008689:Methacrylates; D011108:Polymers; D006046:Gold; C072703:2-aminoethylmethacrylate; D019855:Ethylene Glycol
keywords: non-biofouling synthetic polymers; protein and cellular pattern; surface organic chemistry; surface-functionalizable biomaterials; surface-initiated controlled radical polymerization