title: (Z)-5-(2,4-Dihydroxybenzylidene)thiazolidine-2,4-dione Prevents UVB-Induced Melanogenesis and Wrinkle Formation through Suppressing Oxidative Stress in HRM-2 Hairless Mice.
abstract: Background. Uncontrolled melanogenesis and wrinkle formation are an indication of photoaging. Our previous studies demonstrated that (Z)-5-(2,4-dihydroxybenzylidene)thiazolidine-2,4-dione (MHY498) inhibited tyrosinase activity and melanogenesis in vitro. Objective. To examine in vivo effects of MHY498 as an antiaging compound on UVB-induced melanogenesis and wrinkle formation, we topically applied MHY498 on dorsal skin of HRM-2 hairless mice. Methods. Using histological analysis, we evaluated effects of MHY498 on melanogenesis and wrinkle formation after UVB exposure. In addition, related molecular signaling pathways were examined using western blotting, fluorometric assay, and enzyme-linked immunosorbent assay. Results. MHY498 suppressed UVB-induced melanogenesis by inhibiting phosphorylation of CREB and translocation of MITF protein into the nucleus, which are key factors for tyrosinase expression. Consistently, tyrosinase protein levels were notably reduced in the dorsal skin of the hairless mice by MHY498 treatment. Furthermore, MHY498 inhibited UVB-induced wrinkle formation and collagen fiber destruction by increasing type 1 procollagen concentration and decreasing protein expression levels of MMPs, which play an essential role in collagen fiber degradation. As a mechanism, MHY498 notably ameliorated UVB-induced oxidative stress and NF-κB activation in the dermal skin of the hairless mice. Conclusion. Our study suggests that MHY498 can be used as a therapeutic or cosmetic agent for preventing uncontrolled melanogenesis and wrinkle formation.
authors.affiliation: College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Bonggi
authors.initials: B
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Kyoung Mi
authors.initials: KM
authors.lastname: Moon
authors.affiliation: College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Seong Jin
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: So Hee
authors.initials: SH
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea; Molecular Inflammation Research Center for Ageing Intervention (MRCA), Pusan National University, Republic of Korea.
authors.forename: Dae Hyun
authors.initials: DH
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Hye Jin
authors.initials: HJ
authors.lastname: An
authors.affiliation: College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Ji Won
authors.initials: JW
authors.lastname: Jeong
authors.affiliation: College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Ye Ra
authors.initials: YR
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Sujin
authors.initials: S
authors.lastname: Son
authors.affiliation: College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Min Jo
authors.initials: MJ
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Ki Wung
authors.initials: KW
authors.lastname: Chung
authors.affiliation: College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Eun Kyeong
authors.initials: EK
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Inje University, Busan 609-735, Republic of Korea.
authors.forename: Pusoon
authors.initials: P
authors.lastname: Chun
authors.affiliation: Department of Chemistry, Dong-A University, Busan 609-735, Republic of Korea.
authors.forename: Young Mi
authors.initials: YM
authors.lastname: Ha
authors.affiliation: College of Pharmacy, Sunchon National University, Sunchon, Republic of Korea.
authors.forename: Min-Sun
authors.initials: MS
authors.lastname: Kim
authors.affiliation: Bio-FD&C, Songdo Mirae-ro 30, Incheon, Republic of Korea.
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Mo
authors.affiliation: College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea; Molecular Inflammation Research Center for Ageing Intervention (MRCA), Pusan National University, Republic of Korea.
authors.forename: Hyung Ryong
authors.initials: HR
authors.lastname: Moon
authors.affiliation: College of Pharmacy, Pusan National University, Busan 609-735, Republic of Korea; Molecular Inflammation Research Center for Ageing Intervention (MRCA), Pusan National University, Republic of Korea.
authors.forename: Hae Young
authors.initials: HY
authors.lastname: Chung
mesh_terms: D000818:Animals; D008297:Male; D008543:Melanins / Q000378:metabolism*; D051379:Mice; D008812:Mice, Hairless; D018384:Oxidative Stress / Q000187:drug effects*; D015398:Signal Transduction; D012867:Skin / Q000187:drug effects*; D015595:Skin Aging / Q000187:drug effects*; D045162:Thiazolidinediones / Q000008:administration & dosage / Q000627:therapeutic use*; D014466:Ultraviolet Rays
chemical_list: C585780:(Z)-5-(2,4-dihydroxybenzylidene)thiazolidine-2,4-dione; D008543:Melanins; D045162:Thiazolidinediones