title: Acute hyperlipidemia initiates proinflammatory and proatherogenic changes in circulation and adipose tissue in obese women.
abstract: Obesity represents a high risk factor for the development of atherosclerosis and is associated with a low-grade inflammation and activation of immune cells.
  
  
  
  The aim of our study was to investigate the effect of a short-term lipid infusion on immune cells in blood and subcutaneous abdominal adipose tissue (SAAT) in obese women.
  
  
  
  Seven-hour intravenous lipid/control infusions were performed in two groups of women (n = 15, n = 10, respectively). Before and at the end of the infusion, SAAT and blood samples were obtained and relative content and phenotype of immune cells were analyzed using flow cytometry. Analysis of immune cell markers, inflammation and angiogenesis markers was performed in SAAT by RT-PCR and in plasma by immunoassays.
  
  
  
  Relative content of CD45+/14+ and CD45+/14+/16+ populations of monocytes was reduced in circulation by 21% (p = 0.004) and by 46% (p = 0.0002), respectively, in response to hyperlipidemia, which suggested the increased adhesion of these cells to endothelium. In line with this, the levels of sICAM and sVCAM in plasma were increased by 9.4% (p = 0.016), 11.8% (p = 0.008), respectively. In SAAT, the relative content of M2 monocyte/macrophages subpopulation CD45+/14+/206+/16+ decreased by 27% (p = 0.012) and subpopulations CD14+/CD206- and CD14/+TLR4+ cells increased (p = 0.026; p = 0.049, respectively). Intralipid infusion promoted an increase of mRNA levels in SAAT: RORC (marker of proinflammatory Th17 lymphocytes) by 43% (p = 0.048), MCP-1 (78%, p = 0.028) and VEGF (68.5%, p = 0.0001).
  
  
  
  Acute hyperlipidemia induces a proinflammatory and proatherogenic response associated with altered relative content of immune cells in blood and SAAT in obese women.
authors.affiliation: Department of Sport Medicine, Third Faculty of Medicine, Charles University in Prague, Prague, CZ-100 00, Czech Republic; Franco-Czech Laboratory for Clinical Research on Obesity, Third Faculty of Medicine, Prague, Czech Republic; Second Department of Internal Medicine, University Hospital Královské Vinohrady, Prague, CZ-100 00, Czech Republic; Franco-Czech Laboratory for Clinical Research on Obesity, Institut des Maladies Métaboliques et Cardiovasculaires, Université Toulouse III Paul Sabatier, UMR1048, Toulouse, France.
authors.forename: Eva
authors.initials: E
authors.lastname: Krauzová
authors.affiliation: Department of Sport Medicine, Third Faculty of Medicine, Charles University in Prague, Prague, CZ-100 00, Czech Republic; Franco-Czech Laboratory for Clinical Research on Obesity, Third Faculty of Medicine, Prague, Czech Republic; Franco-Czech Laboratory for Clinical Research on Obesity, Institut des Maladies Métaboliques et Cardiovasculaires, Université Toulouse III Paul Sabatier, UMR1048, Toulouse, France.
authors.forename: Jana
authors.initials: J
authors.lastname: Kračmerová
authors.affiliation: Department of Sport Medicine, Third Faculty of Medicine, Charles University in Prague, Prague, CZ-100 00, Czech Republic; Franco-Czech Laboratory for Clinical Research on Obesity, Third Faculty of Medicine, Prague, Czech Republic; Franco-Czech Laboratory for Clinical Research on Obesity, Institut des Maladies Métaboliques et Cardiovasculaires, Université Toulouse III Paul Sabatier, UMR1048, Toulouse, France.
authors.forename: Lenka
authors.initials: L
authors.lastname: Rossmeislová
authors.affiliation: Department of Sport Medicine, Third Faculty of Medicine, Charles University in Prague, Prague, CZ-100 00, Czech Republic; Franco-Czech Laboratory for Clinical Research on Obesity, Third Faculty of Medicine, Prague, Czech Republic; Franco-Czech Laboratory for Clinical Research on Obesity, Institut des Maladies Métaboliques et Cardiovasculaires, Université Toulouse III Paul Sabatier, UMR1048, Toulouse, France.
authors.forename: Lucia
authors.initials: L
authors.lastname: Mališová
authors.affiliation: Department of Sport Medicine, Third Faculty of Medicine, Charles University in Prague, Prague, CZ-100 00, Czech Republic; Franco-Czech Laboratory for Clinical Research on Obesity, Third Faculty of Medicine, Prague, Czech Republic; Franco-Czech Laboratory for Clinical Research on Obesity, Institut des Maladies Métaboliques et Cardiovasculaires, Université Toulouse III Paul Sabatier, UMR1048, Toulouse, France.
authors.forename: Michaela
authors.initials: M
authors.lastname: Tencerová
authors.affiliation: Department of Sport Medicine, Third Faculty of Medicine, Charles University in Prague, Prague, CZ-100 00, Czech Republic; Franco-Czech Laboratory for Clinical Research on Obesity, Third Faculty of Medicine, Prague, Czech Republic; Franco-Czech Laboratory for Clinical Research on Obesity, Institut des Maladies Métaboliques et Cardiovasculaires, Université Toulouse III Paul Sabatier, UMR1048, Toulouse, France.
authors.forename: Michal
authors.initials: M
authors.lastname: Koc
authors.affiliation: Department of Sport Medicine, Third Faculty of Medicine, Charles University in Prague, Prague, CZ-100 00, Czech Republic; Franco-Czech Laboratory for Clinical Research on Obesity, Third Faculty of Medicine, Prague, Czech Republic; Second Department of Internal Medicine, University Hospital Královské Vinohrady, Prague, CZ-100 00, Czech Republic; Franco-Czech Laboratory for Clinical Research on Obesity, Institut des Maladies Métaboliques et Cardiovasculaires, Université Toulouse III Paul Sabatier, UMR1048, Toulouse, France.
authors.forename: Vladimír
authors.initials: V
authors.lastname: Štich
authors.affiliation: Department of Sport Medicine, Third Faculty of Medicine, Charles University in Prague, Prague, CZ-100 00, Czech Republic; Franco-Czech Laboratory for Clinical Research on Obesity, Third Faculty of Medicine, Prague, Czech Republic; Franco-Czech Laboratory for Clinical Research on Obesity, Institut des Maladies Métaboliques et Cardiovasculaires, Université Toulouse III Paul Sabatier, UMR1048, Toulouse, France. Electronic address: Michaela.Siklova@lf3.cuni.cz.
authors.forename: Michaela
authors.initials: M
authors.lastname: Šiklová
mesh_terms: D000208:Acute Disease; D000273:Adipose Tissue / Q000378:metabolism / Q000473:pathology*; D000328:Adult; D050197:Atherosclerosis / Q000097:blood* / Q000150:complications; D015415:Biomarkers / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood* / Q000150:complications; D007249:Inflammation; D008214:Lymphocytes / Q000166:cytology; D008264:Macrophages / Q000166:cytology; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology; D009765:Obesity / Q000097:blood* / Q000150:complications; D010641:Phenotype; D012333:RNA, Messenger / Q000378:metabolism; D050507:Subcutaneous Fat, Abdominal / Q000378:metabolism / Q000473:pathology*
chemical_list: D015415:Biomarkers; D012333:RNA, Messenger
keywords: Adhesion molecules; Free fatty acids; Human adipose tissue; Hyperlipidemia; Monocytes; Proinflammatory cytokines; T-lymphocytes