title: Fast volumetric imaging with patterned illumination via digital micro-mirror device-based temporal focusing multiphoton microscopy.
abstract: Temporal focusing multiphoton microscopy (TFMPM) has the advantage of area excitation in an axial confinement of only a few microns; hence, it can offer fast three-dimensional (3D) multiphoton imaging. Herein, fast volumetric imaging via a developed digital micromirror device (DMD)-based TFMPM has been realized through the synchronization of an electron multiplying charge-coupled device (EMCCD) with a dynamic piezoelectric stage for axial scanning. The volumetric imaging rate can achieve 30 volumes per second according to the EMCCD frame rate of more than 400 frames per second, which allows for the 3D Brownian motion of one-micron fluorescent beads to be spatially observed. Furthermore, it is demonstrated that the dynamic HiLo structural multiphoton microscope can reject background noise by way of the fast volumetric imaging with high-speed DMD patterned illumination.
authors.affiliation: Department of Engineering Science, National Cheng Kung University, Tainan 701, Taiwan; Center for Micro/Nano Science and Technology, National Cheng Kung University, Tainan 701, Taiwan.
authors.forename: Chia-Yuan
authors.initials: CY
authors.lastname: Chang
authors.affiliation: Department of Photonics, National Cheng Kung University, Tainan 701, Taiwan.
authors.forename: Yvonne Yuling
authors.initials: YY
authors.lastname: Hu
authors.affiliation: Department of Engineering Science, National Cheng Kung University, Tainan 701, Taiwan; Center for Micro/Nano Science and Technology, National Cheng Kung University, Tainan 701, Taiwan.
authors.forename: Chun-Yu
authors.initials: CY
authors.lastname: Lin
authors.affiliation: Department of Engineering Science, National Cheng Kung University, Tainan 701, Taiwan.
authors.forename: Cheng-Han
authors.initials: CH
authors.lastname: Lin
authors.affiliation: Department of Engineering Science, National Cheng Kung University, Tainan 701, Taiwan; Center for Micro/Nano Science and Technology, National Cheng Kung University, Tainan 701, Taiwan.
authors.forename: Hsin-Yu
authors.initials: HY
authors.lastname: Chang
authors.affiliation: Institute of Basic Medical Sciences, National Cheng Kung University, Tainan 701, Taiwan.
authors.forename: Sheng-Feng
authors.initials: SF
authors.lastname: Tsai
authors.affiliation: Faculty of Health and Sport Sciences, University of Tsukuba, Tsukuba 305-8574, Japan.
authors.forename: Tzu-Wei
authors.initials: TW
authors.lastname: Lin
authors.affiliation: Department of Engineering Science, National Cheng Kung University, Tainan 701, Taiwan; Center for Micro/Nano Science and Technology, National Cheng Kung University, Tainan 701, Taiwan; Advanced Optoelectronic Technology Center, National Cheng Kung University, Tainan 701, Taiwan.
authors.forename: Shean-Jen
authors.initials: SJ
authors.lastname: Chen
keywords: (170.3880) Medical and biological imaging; (180.4315) Nonlinear microscopy; (180.6900) Three-dimensional microscopy; (230.4685) Optical microelectromechanical devices