title: Human adipocytes from the subcutaneous superficial layer have greater adipogenic potential and lower PPAR-γ DNA methylation levels than deep layer adipocytes.
abstract: Human subcutaneous fat tissue consists of two layers, superficial adipose tissue (SAT) and deep adipose tissue (DAT). Some recent reports suggest that a disproportionate accumulation of DAT is related to obesity-associated metabolic complications. However, the differences in adipocyte function between SAT and DAT are unclear. To clarify the differences in human adipocyte characteristics between SAT and DAT, human ceiling culture-derived proliferative adipocytes (ccdPAs) were primary cultured from SAT and DAT of three lean female patients. Differences in adipogenic differentiation potential and sensitivity to exogenous adipogenic factors were examined. Epigenetic modification of the CpG island DNA methylation levels of genes related to adipogenesis was measured. In histological analyses, the mean adipocyte size in SAT was significantly larger than that in DAT (8,741 ± 416 vs. 7,732 ± 213 μm(2), P < 0.05). Primary cultured adipocytes from SAT showed significantly greater adipogenesis than did those of DAT. Sensitivity to partial adipogenic stimulation was significantly different between ccdPAs of SAT and DAT. Peroxisome proliferator-activated receptor-γ (PPAR-γ) protein expression and leptin protein secretion from ccdPAs were significantly higher in SAT than DAT. DNA methylation levels of PPAR-γ were significantly lower in ccdPAs of SAT than DAT. Adipocyte size was larger in SAT than DAT in vivo. This is consistent with the findings of an in vitro study that, compared with ccdPAs in DAT, ccdPAs in SAT have higher adipogenic potential and lower DNA methylation levels of PPAR-γ.
authors.affiliation: Department of Plastic Surgery, Chiba University Chuo-ku, Chiba-city, Chiba, Japan;
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kosaka
authors.affiliation: Department of Plastic Surgery, Chiba University Chuo-ku, Chiba-city, Chiba, Japan; kubota-cbu@umin.ac.jp.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Kubota
authors.affiliation: Department of Plastic Surgery, Chiba University Chuo-ku, Chiba-city, Chiba, Japan;
authors.forename: Naoki
authors.initials: N
authors.lastname: Adachi
authors.affiliation: Department of Plastic Surgery, Chiba University Chuo-ku, Chiba-city, Chiba, Japan;
authors.forename: Shinsuke
authors.initials: S
authors.lastname: Akita
authors.affiliation: Department of Plastic Surgery, Chiba University Chuo-ku, Chiba-city, Chiba, Japan;
authors.forename: Yoshitaro
authors.initials: Y
authors.lastname: Sasahara
authors.affiliation: Department of Plastic Surgery, Chiba University Chuo-ku, Chiba-city, Chiba, Japan;
authors.forename: Tomoe
authors.initials: T
authors.lastname: Kira
authors.affiliation: Center for Advanced Medicine, Chiba University, Chiba-city, Chiba, Japan;
authors.forename: Masayuki
authors.initials: M
authors.lastname: Kuroda
authors.affiliation: Department of Plastic Surgery, Chiba University Chuo-ku, Chiba-city, Chiba, Japan;
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Mitsukawa
authors.affiliation: Department of Research and Development, Toho University, Sakura-city, Chiba, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Bujo
authors.affiliation: Department of Plastic Surgery, Chiba University Chuo-ku, Chiba-city, Chiba, Japan;
authors.forename: Kaneshige
authors.initials: K
authors.lastname: Satoh
mesh_terms: D017667:Adipocytes / Q000378:metabolism* / Q000502:physiology*; D050156:Adipogenesis / Q000502:physiology*; D000273:Adipose Tissue / Q000378:metabolism / Q000502:physiology; D000328:Adult; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D002478:Cells, Cultured; D018899:CpG Islands / Q000235:genetics; D019175:DNA Methylation / Q000502:physiology*; D005260:Female; D006801:Humans; D020738:Leptin / Q000378:metabolism; D008875:Middle Aged; D009765:Obesity / Q000378:metabolism / Q000503:physiopathology; D047495:PPAR gamma / Q000378:metabolism*; D050151:Subcutaneous Fat / Q000378:metabolism* / Q000502:physiology*
chemical_list: D020738:Leptin; D047495:PPAR gamma
keywords: adipocyte; adipogenesis; epigenetics; subcutaneous tissue