title: Neutralization Assay for Chikungunya Virus Infection: Plaque Reduction Neutralization Test.
abstract: Neutralization assay is a technique that detects and quantifies neutralizing antibody in serum samples by calculating the percentage of reduction of virus activity, as the concentration of virus used is usually constant. Neutralizing antibody titer is conventionally determined by calculating the percentage reduction in total virus infectivity by counting and comparing number of plaques (localized area of infection due to cytopathic effect) with a standard amount of virus. Conventional neutralizing test uses plaque-reduction neutralization test (PRNT) to determine neutralizing antibody titers against Chikungunya virus (CHIKV). Here we describe the plaque reduction neutralization assay (PRNT) using Vero cell lines to obtain neutralizing antibody titers.
authors.affiliation: Department of Virology 1, National Institute of Infectious Diseases, Toyama 1-23-1 Shinjuku-ku, Tokyo, 162-8640, Japan.|Graduate School for Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8577, Japan.
authors.forename: Nor Azila Muhammad
authors.initials: NA
authors.lastname: Azami
authors.affiliation: Department of Virology 1, National Institute of Infectious Diseases, Toyama 1-23-1 Shinjuku-ku, Tokyo, 162-8640, Japan. sherry@nagasaki-u.ac.jp.|Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan. sherry@nagasaki-u.ac.jp.
authors.forename: Meng Ling
authors.initials: ML
authors.lastname: Moi
authors.affiliation: Department of Virology 1, National Institute of Infectious Diseases, Toyama 1-23-1 Shinjuku-ku, Tokyo, 162-8640, Japan.
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Takasaki
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood*; D000914:Antibodies, Viral / Q000097:blood*; D002460:Cell Line; D065632:Chikungunya Fever / Q000097:blood* / Q000276:immunology; D002646:Chikungunya virus / Q000276:immunology*; D002522:Chlorocebus aethiops; D006801:Humans; D009500:Neutralization Tests / Q000379:methods*; D014709:Vero Cells; D010948:Viral Plaque Assay
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral
keywords: Antibody; Chikungunya; Neutralizing antibody; Neutralizing assay; Plaque reduction neutralization test; Vero cell lines