title: Engineering WT1-Encoding mRNA to Increase Translational Efficiency in Dendritic Cells.
abstract: Dendritic cells (DCs) are the orchestrators of the immune system and are frequently used in clinical trials in order to boost the immune system in cancer patients. Among several available techniques for DC modification, mRNA electroporation is an interesting technique due to the favorable characteristics of mRNA. Antigen expression level and duration can be increased by multiple optimizations of an antigen-encoding mRNA template. Here, we describe different molecular modifications to a WT1-encoding mRNA construct in order to increase antigen expression and the subsequent introduction of mRNA into DCs.
authors.affiliation: Laboratory of Molecular and Cellular Therapy, Department of Immunology-Physiology and the Dendritic Cell Bank, Vrije Universiteit Brussel, Laarbeeklaan 103/E235, 1090, Brussels, Belgium. Daphne.benteyn@vub.ac.be.
authors.forename: Daphné
authors.initials: D
authors.lastname: Benteyn
authors.affiliation: Laboratory of Molecular and Cellular Therapy, Department of Immunology-Physiology and the Dendritic Cell Bank, Vrije Universiteit Brussel, Laarbeeklaan 103/E235, 1090, Brussels, Belgium.
authors.forename: Carlo
authors.initials: C
authors.lastname: Heirman
authors.affiliation: Laboratory of Molecular and Cellular Therapy, Department of Immunology-Physiology and the Dendritic Cell Bank, Vrije Universiteit Brussel, Laarbeeklaan 103/E235, 1090, Brussels, Belgium.
authors.forename: Kris
authors.initials: K
authors.lastname: Thielemans
authors.affiliation: Laboratory of Molecular and Cellular Therapy, Department of Immunology-Physiology and the Dendritic Cell Bank, Vrije Universiteit Brussel, Laarbeeklaan 103/E235, 1090, Brussels, Belgium.
authors.forename: Aude
authors.initials: A
authors.lastname: Bonehill
mesh_terms: D003713:Dendritic Cells / Q000378:metabolism*; D018274:Electroporation; D005818:Genetic Engineering / Q000379:methods*; D006801:Humans; D014176:Protein Biosynthesis; D012315:RNA Caps / Q000378:metabolism; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D025721:WT1 Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D012315:RNA Caps; D012333:RNA, Messenger; D025721:WT1 Proteins; C583751:WT1 protein, human
keywords: Antigen expression; Cancer; Dendritic cells; Immunotherapy; Vaccination; Wilms’ Tumor 1; mRNA