title: In vitro assessment of a calcium-fluoroaluminosilicate glass-based desensitizer for the prevention of root surface demineralization.
abstract: The purpose of this study was to evaluate the ability of a calcium-fluoroaluminosilicate glass-based desensitizer (Nanoseal) to protect against root demineralization in vitro. Nanoseal was applied to human root dentin, which was immersed in acidic buffer for 72 h, or exposed to pH cycling by immersing in distilled water or mineralizing solution for 24 h intermediately during 48 h-acid attack. Demineralization was evaluated by μ-CT, and mineral loss (ML) and lesion depth (LD) were determined from mineral density profiles. ML and LD in all treatment groups were significantly smaller compared with control. The Nanoseal-treated group with pH cycling using mineralizing solution had the lowest ML and LD. Analysis using an EPMA demonstrated calcium and phosphorous were incorporated into the superficial layer of specimens in the Nanoseal-treated groups, suggesting Nanoseal modified the dentin surface, making it resistant to demineralization. Application of Nanoseal is an effective method for protecting root from demineralization.
authors.affiliation: Department of Biomaterials Science, Osaka University Graduate School of Dentistry.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Miyajima
authors.forename: Takuya
authors.initials: T
authors.lastname: Ishimoto
authors.forename: Sai
authors.initials: S
authors.lastname: Ma
authors.forename: Jihua
authors.initials: J
authors.lastname: Chen
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Nakano
authors.forename: Satoshi
authors.initials: S
authors.lastname: Imazato
mesh_terms: D017607:Aluminum Compounds; D002118:Calcium; D002327:Cariostatic Agents; D003804:Dentin; D005459:Fluorides; D006801:Humans; D017655:Silicon Compounds; D017001:Tooth Demineralization; D014092:Tooth Root
chemical_list: D017607:Aluminum Compounds; D002327:Cariostatic Agents; D017655:Silicon Compounds; C020328:fluoroaluminum silicate; D005459:Fluorides; D002118:Calcium