title: Application of oleosin-flanked keratinocyte growth factor-2 expressed from Arabidopsis thaliana promotes hair follicle growth in mice.
abstract: OBJECTIVE
  To determine an efficient expression strategy in Arabidopsis thaliana and to determine whether a dimeric oleosin fusion approach could achieve keratinocyte growth factor-2 (KGF2) expression and bioactivity.
  
  
  RESULTS
  Higher recombinant protein accumulation was observed in the two dimeric oleosin constructs than in a single-oleosin-fused protein (O::KGR2) or KGF2 control. Highest expression was in O-O::KGF2-transgenic seeds. MTT assay in FGFR2 III b-BaF3 cells revealed comparable levels of bioactivity due to O-O::KGF2 and O::KGR2, whereas O::KGF2-O had no effect on FGFR2 III b-BaF3 opithelial cell growth. The transgenic proteins had a pronounced stimulatory effect on hair follicle proliferation in C57BL/6 mice.
  
  
  CONCLUSIONS
  A dimeric oleosin approach can be used to express KGF2 with the non-symmetrical O-O::KGF2 construct showing the highest expression and bioactivity.
authors.affiliation: Engineering Research Center of Bioreactor and Pharmaceutical Development Ministry of Education, Jilin Agricultural University, Changchun, 130118, Jilin, China.|School of Pharmaceutical Science, Key Laboratory of Biotechnology and Pharmaceutical Engineering of Zhejiang Province, Wenzhou Medical College, Wenzhou, 325035, Zhejiang, China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: School of Pharmaceutical Science, Key Laboratory of Biotechnology and Pharmaceutical Engineering of Zhejiang Province, Wenzhou Medical College, Wenzhou, 325035, Zhejiang, China.
authors.forename: Shenghui
authors.initials: S
authors.lastname: Chu
authors.affiliation: School of Pharmaceutical Science, Key Laboratory of Biotechnology and Pharmaceutical Engineering of Zhejiang Province, Wenzhou Medical College, Wenzhou, 325035, Zhejiang, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ai
authors.affiliation: Engineering Research Center of Bioreactor and Pharmaceutical Development Ministry of Education, Jilin Agricultural University, Changchun, 130118, Jilin, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Li
authors.affiliation: School of Pharmaceutical Science, Key Laboratory of Biotechnology and Pharmaceutical Engineering of Zhejiang Province, Wenzhou Medical College, Wenzhou, 325035, Zhejiang, China.
authors.forename: Zhiyu
authors.initials: Z
authors.lastname: Chen
authors.affiliation: School of Pharmaceutical Science, Key Laboratory of Biotechnology and Pharmaceutical Engineering of Zhejiang Province, Wenzhou Medical College, Wenzhou, 325035, Zhejiang, China.
authors.forename: Sisi
authors.initials: S
authors.lastname: Huang
authors.affiliation: Engineering Research Center of Bioreactor and Pharmaceutical Development Ministry of Education, Jilin Agricultural University, Changchun, 130118, Jilin, China. Changjiang10@hotmail.com.|School of Pharmaceutical Science, Key Laboratory of Biotechnology and Pharmaceutical Engineering of Zhejiang Province, Wenzhou Medical College, Wenzhou, 325035, Zhejiang, China. Changjiang10@hotmail.com.
authors.forename: Chao
authors.initials: C
authors.lastname: Jiang
authors.affiliation: Engineering Research Center of Bioreactor and Pharmaceutical Development Ministry of Education, Jilin Agricultural University, Changchun, 130118, Jilin, China. proflxk@163.com.
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D051526:Fibroblast Growth Factor 10 / Q000378:metabolism* / Q000494:pharmacology*; D018859:Hair Follicle / Q000187:drug effects*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism
chemical_list: D051526:Fibroblast Growth Factor 10
keywords: Arabidopsis thaliana; Expression and Bioactivity; Hair follicle growth; Keratinocyte growth factor-2; Oleosin