title: Expression of dmrt1 and vtg genes during gonad formation, differentiation and early maturation in cultured Russian sturgeon Acipenser gueldenstaedtii.
abstract: Expression of the dmrt1 and vtg genes was described using the real-time PCR (rt-PCR) method from 25 to 1600 days post-hatch (dph) in cultured Russian sturgeon Acipenser gueldenstaedtii. The level of dmrt1 transcription in gonads in subsequent studied periods increased exponentially while vtg expression increased in gonads and livers of A. gueldenstaedtii examined, but in later stages of development. Both dmrt1 and vtg genes showed elevated expression in intersex individuals probably caused by dietary exposure to phyto-oestrogens.
authors.affiliation: Department of Ichthyobiology, Fisheries and Aquaculture Biotechnology, Faculty of Animal Science, Warsaw University of Life Sciences, 02-786, Warsaw, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Fajkowska
authors.affiliation: Department of Ichthyobiology, Fisheries and Aquaculture Biotechnology, Faculty of Animal Science, Warsaw University of Life Sciences, 02-786, Warsaw, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Rzepkowska
authors.affiliation: Department of Ichthyobiology, Fisheries and Aquaculture Biotechnology, Faculty of Animal Science, Warsaw University of Life Sciences, 02-786, Warsaw, Poland.
authors.forename: D
authors.initials: D
authors.lastname: Adamek
authors.affiliation: Department of Ichthyobiology, Fisheries and Aquaculture Biotechnology, Faculty of Animal Science, Warsaw University of Life Sciences, 02-786, Warsaw, Poland.
authors.forename: T
authors.initials: T
authors.lastname: Ostaszewska
authors.affiliation: Department of Sturgeon Fish Breeding, Inland Fisheries Institute in Olsztyn, Pieczarki 50, 11-610, Pozezdrze, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Szczepkowski
mesh_terms: D000818:Animals; D005260:Female; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D005399:Fishes / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D006066:Gonads / Q000254:growth & development / Q000378:metabolism*; D008297:Male; D019849:Sex Determination Processes; D012733:Sex Differentiation; D014157:Transcription Factors / Q000378:metabolism*; D014819:Vitellogenins / Q000378:metabolism*
chemical_list: C110547:DMRT1 protein; D029941:Fish Proteins; D014157:Transcription Factors; D014819:Vitellogenins
keywords: dmrt1; gonad differentiation; intersex; phyto-oestrogen; vitellogenin