title: Glycine confers neuroprotection through microRNA-301a/PTEN signaling.
abstract: Glycine is known to protect against neuronal death. However, the underlying mechanism remains to be elucidated. The microRNA-301a is involved in both biological and pathological processes. But it is not known whether microRNA-301a has a neuroprotective property. In this study, we aimed to determine whether glycine-induced neuroprotection requires microRNA-301a-dependent signaling.
  
  
  
  We provided the first evidence that glycine increased the expression of microRNA-301a in cultured rat cortical neurons and protected against cortical neuronal death through up-regulation of microRNA-301a after oxygen-glucose deprivation. MicroRNA-301a directly bound the predicted 3'UTR target sites of PTEN and reduced PTEN expression in cortical neurons. We revealed that PTEN down-regulation by microRNA-301a mediated glycine-induced neuroprotective effect following oxygen-glucose deprivation.
  
  
  
  Our results suggest that 1) microRNA-301a is neuroprotective in oxygen-glucose deprivation-induced neuronal injury; 2) glycine is an upstream regulator of microRNA-301a; 3) glycine confers neuroprotection through microRNA-301a/PTEN signal pathway.
authors.affiliation: Department of Physiology, School of Basic Medical Sciences, Medical Research Institute, Wuhan University School of Medicine, 185 Donghu Street, Wuhan, 430071, China.|Department of Neurology, the Central Hospital of Wuhan, Wuhan, 430060, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Physiology, School of Basic Medical Sciences, Medical Research Institute, Wuhan University School of Medicine, 185 Donghu Street, Wuhan, 430071, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhuang
authors.affiliation: Department of Physiology, School of Basic Medical Sciences, Medical Research Institute, Wuhan University School of Medicine, 185 Donghu Street, Wuhan, 430071, China.
authors.forename: Zhi-Feng
authors.initials: ZF
authors.lastname: Zhang
authors.affiliation: Department of Physiology, School of Basic Medical Sciences, Medical Research Institute, Wuhan University School of Medicine, 185 Donghu Street, Wuhan, 430071, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Neurology, the Central Hospital of Wuhan, Wuhan, 430060, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Jin
authors.affiliation: Department of Genetics, School of Basic Medical Sciences, Wuhan University School of Medicine, 185 Donghu Street, Wuhan, 430071, China.
authors.forename: Chunjiang
authors.initials: C
authors.lastname: He
authors.affiliation: Department of Physiology, School of Basic Medical Sciences, Medical Research Institute, Wuhan University School of Medicine, 185 Donghu Street, Wuhan, 430071, China.
authors.forename: Peng-Chao
authors.initials: PC
authors.lastname: Hu
authors.affiliation: Department of Physiology, School of Basic Medical Sciences, Medical Research Institute, Wuhan University School of Medicine, 185 Donghu Street, Wuhan, 430071, China.
authors.forename: Ze-Fen
authors.initials: ZF
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Zhongnan Hospital, Wuhan University School of Medicine, 169 Donghu Street, Wuhan, 430071, China.
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Li
authors.affiliation: Department of Neurology, the Central Hospital of Huanggang, Huanggang, 438000, China.
authors.forename: Guang-Ming
authors.initials: GM
authors.lastname: Xia
authors.affiliation: Department of Neurology, the Central Hospital of Huanggang, Huanggang, 438000, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Physiology, School of Basic Medical Sciences, Medical Research Institute, Wuhan University School of Medicine, 185 Donghu Street, Wuhan, 430071, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Physiology, School of Basic Medical Sciences, Medical Research Institute, Wuhan University School of Medicine, 185 Donghu Street, Wuhan, 430071, China. qwan@whu.edu.cn.|Department of Neurosurgery, Zhongnan Hospital, Wuhan University School of Medicine, 169 Donghu Street, Wuhan, 430071, China. qwan@whu.edu.cn.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wan
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D002540:Cerebral Cortex / Q000473:pathology; D005786:Gene Expression Regulation / Q000187:drug effects; D005947:Glucose / Q000172:deficiency; D005998:Glycine / Q000494:pharmacology*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects / Q000473:pathology; D000066829:Neuroprotection / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000494:pharmacology; D020411:Oligonucleotide Array Sequence Analysis; D010100:Oxygen; D051059:PTEN Phosphohydrolase / Q000235:genetics / Q000378:metabolism*; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects* / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; C000624542:MIRN301 microRNA, rat; D035683:MicroRNAs; D018696:Neuroprotective Agents; D051059:PTEN Phosphohydrolase; D005947:Glucose; D010100:Oxygen; D005998:Glycine
keywords: Glycine; Neuroprotection; PTEN; miR-301a