title: 1,2-Propanediol Dehydration in Roseburia inulinivorans: STRUCTURAL BASIS FOR SUBSTRATE AND ENANTIOMER SELECTIVITY.
abstract: Glycyl radical enzymes (GREs) represent a diverse superfamily of enzymes that utilize a radical mechanism to catalyze difficult, but often essential, chemical reactions. In this work we present the first biochemical and structural data for a GRE-type diol dehydratase from the organism Roseburia inulinivorans (RiDD). Despite high sequence (48% identity) and structural similarity to the GRE-type glycerol dehydratase from Clostridium butyricum, we demonstrate that the RiDD is in fact a diol dehydratase. In addition, the RiDD will utilize both (S)-1,2-propanediol and (R)-1,2-propanediol as a substrate, with an observed preference for the S enantiomer. Based on the new structural information we developed and successfully tested a hypothesis that explains the functional differences we observe.
authors.affiliation: From the Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602 and.
authors.forename: Joseph W
authors.initials: JW
authors.lastname: LaMattina
authors.affiliation: From the Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602 and.
authors.forename: Nicholas D
authors.initials: ND
authors.lastname: Keul
authors.affiliation: From the Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602 and.
authors.forename: Pierre
authors.initials: P
authors.lastname: Reitzer
authors.affiliation: From the Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602 and.
authors.forename: Suraj
authors.initials: S
authors.lastname: Kapoor
authors.affiliation: BRASKEM S.A., Rua Lemos Moonteiro, 120 Edifício Odebrecht São Paulo, Butantã 05501-050-São Paulo, SP Brasil.
authors.forename: Felipe
authors.initials: F
authors.lastname: Galzerani
authors.affiliation: BRASKEM S.A., Rua Lemos Moonteiro, 120 Edifício Odebrecht São Paulo, Butantã 05501-050-São Paulo, SP Brasil.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Koch
authors.affiliation: BRASKEM S.A., Rua Lemos Moonteiro, 120 Edifício Odebrecht São Paulo, Butantã 05501-050-São Paulo, SP Brasil.
authors.forename: Iuri E
authors.initials: IE
authors.lastname: Gouvea
authors.affiliation: From the Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602 and wlanzilo@bmb.uga.edu.
authors.forename: William N
authors.initials: WN
authors.lastname: Lanzilotta
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D000068016:Eubacteriales / Q000201:enzymology* / Q000235:genetics; D011408:Propanediol Dehydratase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D019946:Propylene Glycol / Q000737:chemistry* / Q000378:metabolism; D013379:Substrate Specificity / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; D019946:Propylene Glycol; D011408:Propanediol Dehydratase
keywords: chemical biology; diol dehydratase; enzyme structure; glycerol dehydratase; glycyl radical enzyme; intestinal metabolism; metabolic engineering; radical; radical dehydration