title: Diacylglycerol acyl transferase: A pathogenicity related gene in Colletotrichum gloeosporioides.
abstract: To gain more insight into the molecular mechanisms of Colletotrichum gloeosporioides pathogenesis, restriction enzyme-mediated integration (REMI) mutagenesis identified the mutants of C. gloeosporioides impaired in pathogenicity. Transformants screened for defects in pathogenicity using detached leaves and fruits. Of the 20 REMI transformants tested, two mutants (H4 and H7) showed reduced pathogenicity on leaves of apple, kiwi, mango, peach, and fruits of guava, apple, and capsicum. One tagged gene from the genome sequence of mutant H4 was recovered by inverse PCR. Sequence analysis of the tagged site in mutant H4 revealed insertion in diacylglycerol acyltransferase gene which encodes diacylglycerol acyltransferase enzyme, catalyzing the steps involved in the biosynthesis of triacylglycerol, an important component of biological membranes and source of energy. Therefore, tagging of diacylglycerol acyltransferase gene in mutant H4 resulted in reduced pathogenicity, indicating possible role of this gene in pathogenicity of C. gloeosporioides.
authors.affiliation: Faculty of Applied Sciences and Biotechnology, Shoolini University of Biotechnology and Management Sciences, Bajhol, Solan, Himachal Pradesh, India.
authors.forename: Meenakshi
authors.initials: M
authors.lastname: Sharma
authors.affiliation: Faculty of Applied Sciences and Biotechnology, Shoolini University of Biotechnology and Management Sciences, Bajhol, Solan, Himachal Pradesh, India.
authors.forename: Shiwani
authors.initials: S
authors.lastname: Guleria
authors.affiliation: Faculty of Applied Sciences and Biotechnology, Shoolini University of Biotechnology and Management Sciences, Bajhol, Solan, Himachal Pradesh, India.
authors.forename: Saurabh
authors.initials: S
authors.lastname: Kulshrestha
mesh_terms: D029042:Actinidia / Q000382:microbiology; D001483:Base Sequence; D002934:Cinnamates / Q000494:pharmacology; D020231:Colletotrichum / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D051048:Diacylglycerol O-Acyltransferase / Q000378:metabolism*; D016681:Genome, Fungal; D006921:Hygromycin B / Q000031:analogs & derivatives / Q000494:pharmacology; D027845:Malus / Q000382:microbiology; D016254:Mutagenesis, Insertional; D009154:Mutation; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000382:microbiology; D016133:Polymerase Chain Reaction; D014774:Virulence / Q000235:genetics
chemical_list: D002934:Cinnamates; D006921:Hygromycin B; C026273:hygromycin A; D051048:Diacylglycerol O-Acyltransferase; D004262:DNA Restriction Enzymes
keywords: Colletotrichum gloeosporioides; Diacylglycerol acyltransferase; Pathogenesis; REMI