title: Immune Monitoring Using mRNA-Transfected Dendritic Cells.
abstract: Dendritic cells are known to be the most potent antigen presenting cell in the immune system and are used as cellular adjuvants in therapeutic anticancer vaccines using various tumor-associated antigens or their derivatives. One way of loading antigen into the dendritic cells is by mRNA electroporation, ensuring presentation of antigen through major histocompatibility complex I and potentially activating T cells, enabling them to kill the tumor cells. Despite extensive research in the field, only one dendritic cell-based vaccine has been approved. There is therefore a great need to elucidate and understand the immunological impact of dendritic cell vaccination in order to improve clinical benefit. In this chapter, we describe a method for performing immune monitoring using peripheral blood mononuclear cells and autologous dendritic cells transfected with tumor-associated antigen-encoding mRNA.
authors.affiliation: Department of Haematology, Center for Cancer Immune Therapy, Copenhagen University Hospital Herlev, Herlev Ringvej 75, 2730, Herlev, Denmark. troels.holz.borch@regionh.dk.|Department of Oncology, Copenhagen University Hospital Herlev, Herlev, Denmark. troels.holz.borch@regionh.dk.
authors.forename: Troels Holz
authors.initials: TH
authors.lastname: Borch
authors.affiliation: Department of Haematology, Center for Cancer Immune Therapy, Copenhagen University Hospital Herlev, Herlev Ringvej 75, 2730, Herlev, Denmark.|Department of Oncology, Copenhagen University Hospital Herlev, Herlev, Denmark.
authors.forename: Inge Marie
authors.initials: IM
authors.lastname: Svane
authors.affiliation: Department of Haematology, Center for Cancer Immune Therapy, Copenhagen University Hospital Herlev, Herlev Ringvej 75, 2730, Herlev, Denmark.|Department of Oncology, Copenhagen University Hospital Herlev, Herlev, Denmark.
authors.forename: Özcan
authors.initials: Ö
authors.lastname: Met
mesh_terms: D017951:Antigen Presentation; D000951:Antigens, Neoplasm / Q000235:genetics / Q000276:immunology*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000166:cytology; D015166:Monitoring, Immunologic; D012333:RNA, Messenger / Q000235:genetics* / Q000276:immunology; D014162:Transfection; D014611:Vaccination
chemical_list: D000951:Antigens, Neoplasm; C573415:IFNG protein, human; D012333:RNA, Messenger; D007371:Interferon-gamma
keywords: Cancer immunotherapy; Dendritic cell; Electroporation; Immune monitoring; Vaccination; mRNA transfection