title: miR-134 inhibits non-small cell lung cancer growth by targeting the epidermal growth factor receptor.
abstract: The epidermal growth factor receptor (EGFR) is frequently activated in a wide range of solid tumours and represents an important therapeutic target. MicroRNAs (miRNAs) have recently been recognized as a rational and potential modality for anti-EGFR therapies. However, more EGFR-targeting miRNAs need to be explored. In this study, we identified a novel EGFR-targeting miRNA, miRNA-134 (miR-134), in non-small-cell lung cancer (NSCLC) cell lines. Luciferase assays confirmed that EGFR is a direct target of miR-134. In addition, the overexpression of miR-134 inhibited EGFR-related signaling and suppressed NSCLC cells proliferation by inducing cell cycle arrest and/or apoptosis, suggesting that miR-134 functions as a tumour suppressor in NSCLC. Further mechanistic investigation including RNAi and rescue experiments suggested that the down-regulation of EGFR by miR-134 partially contributes to the antiproliferative role of miR-134. Last, in vivo experiments demonstrated that miR-134 suppressed tumour growth of A549 xenograft in nude mice. Taken together, our findings suggest that miR-134 inhibits non-small cell lung cancer growth by targeting the EGFR.
authors.affiliation: Department of Radiation Oncology (Chest Section), Shandong Cancer Hospital and Institute, Shandong University, Jinan, China.|Department of Radiation Oncology (Chest Section), Shandong Cancer Hospital and Institute, Shandong Academy of Medical Sciences, Jinan, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Qin
authors.affiliation: Institute of Basic Medicine, Shandong Academy of Medical Sciences, School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, Jinan, China.
authors.forename: Furong
authors.initials: F
authors.lastname: Wei
authors.affiliation: Department of Pathology, Shandong Cancer Hospital and Institute, Shandong University, Jinan, China.
authors.forename: Jianbo
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Basic Research Center, Shandong Cancer Hospital and Institute, Shandong University, Jinan, China.
authors.forename: Xingwu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Radiation Oncology (Chest Section), Shandong Cancer Hospital and Institute, Shandong University, Jinan, China. baoshli1963@163.com.|Department of Radiation Oncology (Chest Section), Shandong Cancer Hospital and Institute, Shandong Academy of Medical Sciences, Jinan, China. baoshli1963@163.com.
authors.forename: Baosheng
authors.initials: B
authors.lastname: Li
mesh_terms: D000818:Animals; D001483:Base Sequence; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D015536:Down-Regulation; D066246:ErbB Receptors / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology*; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D023041:Xenograft Model Antitumor Assays
chemical_list: C569273:MIRN134 microRNA, human; D035683:MicroRNAs; C512478:EGFR protein, human; D066246:ErbB Receptors
keywords: EGFR; miR-134; non-small cell lung cancer; tumour suppressor