title: Ultrasound-guided photoacoustic imaging for the selective detection of EGFR-expressing breast cancer and lymph node metastases.
abstract: We assessed the use of ultrasound (US)-guided photoacoustic imaging (PAI) and anti-EGFR antibody-conjugated gold nanorods (anti-EGFR-GNs) to non-invasively detect EGFR-expressing primary tumor masses and regional lymph node (LN) metastases in breast tumor mice generated by injecting MCF-7 (EGFR-negative) or MDA-MB-231 (EGFR-positive) human breast cells using a preclinical Vevo 2100 LAZR Imaging system. Anti-EGFR-GNs provided a significant enhancement in the PA signal in MDA-MB-231 tumor and the axillary LN metastases relative to MCF-7 tumor and non-LN metastases. We demonstrated that US-guided PAI using anti-EGFR-GNs is highly sensitive for the selective visualization of EGFR-expressing breast primary tumors as well as LN micrometastases.
authors.affiliation: Department of Science and Radiology, College of Medicine, Seoul National University, 103 Daehak-ro, Jongno-gu, Seoul 03080, South Korea; Contributed equally.
authors.forename: Meihua
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Radiology, Seoul National University Hospital, 101 Daehak-ro, Jongno-gu, Seoul 03080, South Korea; Contributed equally.
authors.forename: Hoe Suk
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Department of Radiology, Seoul National University Hospital, 101 Daehak-ro, Jongno-gu, Seoul 03080, South Korea.
authors.forename: Tiefeng
authors.initials: T
authors.lastname: Jin
authors.affiliation: Seoul National University Hospital HealthCare System Gangnam Center, 152 Teheran-ro, Gangnam-gu, Seoul 06236, South Korea; an_nie@naver.com.
authors.forename: Ann
authors.initials: A
authors.lastname: Yi
authors.affiliation: Department of Science and Radiology, College of Medicine, Seoul National University, 103 Daehak-ro, Jongno-gu, Seoul 03080, South Korea; Department of Radiology, Seoul National University Hospital, 101 Daehak-ro, Jongno-gu, Seoul 03080, South Korea; moonwk@snu.ac.kr.
authors.forename: Woo Kyung
authors.initials: WK
authors.lastname: Moon
keywords: (170.3880) Medical and biological imaging; (170.4580) Optical diagnostics for medicine; (170.5120) Photoacoustic imaging; (170.7170) Ultrasound