title: Infrapatellar Fat Pad: An Alternative Source of Adipose-Derived Mesenchymal Stem Cells.
abstract: Introduction. The Infrapatellar fat pad (IPFP) represents an emerging alternative source of adipose-derived mesenchymal stem cells (ASCs). We compared the characteristics and differentiation capacity of ASCs isolated from IPFP and SC. Materials and Methods. ASCs were harvested from either IPFP or SC. IPFPs were collected from patients undergoing total knee arthroplasty (TKA), whereas subcutaneous tissues were collected from patients undergoing lipoaspiration. Immunophenotypes of surface antigens were evaluated. Their ability to form colony-forming units (CFUs) and their differentiation potential were determined. The ASCs karyotype was evaluated. Results. There was no difference in the number of CFUs and size of CFUs between IPFP and SC sources. ASCs isolated from both sources had a normal karyotype. The mesenchymal stem cells (MSCs) markers on flow cytometry was equivalent. IPFP-ASCs demonstrated significantly higher expression of SOX-9 and RUNX-2 over ASCs isolated from SC (6.19 ± 5.56-, 0.47 ± 0.62-fold; p value = 0.047, and 17.33 ± 10.80-, 1.56 ± 1.31-fold; p value = 0.030, resp.). Discussion and Conclusion. CFU assay of IPFP-ASCs and SC-ASCs harvested by lipoaspiration technique was equivalent. The expression of key chondrogenic and osteogenic genes was increased in cells isolated from IPFP. IPFP should be considered a high quality alternative source of ASCs.
authors.affiliation: Department of Orthopaedics, Faculty of Medicine, Ramathibodi Hospital, Bangkok 10400, Thailand.
authors.forename: P
authors.initials: P
authors.lastname: Tangchitphisut
authors.affiliation: Office of Research and Innovation, Ramathibodi Hospital, Bangkok 10400, Thailand.
authors.forename: N
authors.initials: N
authors.lastname: Srikaew
authors.affiliation: Department of Surgery, Faculty of Medicine, Ramathibodi Hospital, Bangkok 10400, Thailand.
authors.forename: S
authors.initials: S
authors.lastname: Numhom
authors.affiliation: Office of Research and Innovation, Ramathibodi Hospital, Bangkok 10400, Thailand.
authors.forename: A
authors.initials: A
authors.lastname: Tangprasittipap
authors.affiliation: Department of Orthopaedics, Faculty of Medicine, Ramathibodi Hospital, Bangkok 10400, Thailand.
authors.forename: P
authors.initials: P
authors.lastname: Woratanarat
authors.affiliation: Department of Orthopaedics, Faculty of Medicine, Ramathibodi Hospital, Bangkok 10400, Thailand.
authors.forename: S
authors.initials: S
authors.lastname: Wongsak
authors.affiliation: Department of Orthopaedics, Faculty of Medicine, Ramathibodi Hospital, Bangkok 10400, Thailand.
authors.forename: C
authors.initials: C
authors.lastname: Kijkunasathian
authors.affiliation: Department of Pediatrics, Faculty of Medicine, Ramathibodi Hospital, Bangkok 10400, Thailand.
authors.forename: S
authors.initials: S
authors.lastname: Hongeng
authors.affiliation: Department of Orthopaedics, Royal Infirmary Edinburgh Hospital, Edinburgh University, Edinburgh, UK.
authors.forename: I R
authors.identifier: 0000-0003-4637-6356
authors.initials: IR
authors.lastname: Murray
authors.affiliation: Department of Orthopaedics, Faculty of Medicine, Ramathibodi Hospital, Bangkok 10400, Thailand.
authors.forename: T
authors.identifier: 0000-0002-9366-8039
authors.initials: T
authors.lastname: Tawonsawatruk