title: Cellular localization of Sun4p and its interaction with proteins in the yeast birth scar.
abstract: Yeast harbor several proteins with predicted glucanase activity that are potentially involved in cell wall remodeling during different processes, including mitosis. Here, we showed that 2 of these putative glucanases, Sun4p and Dse2p, co-localize to the yeast birth scar, dependently on presence of the third glucanase, Egt2p. The absence of these glucanases results in inefficient mother-daughter cell separation. The Sun4p, Dse2p and Egt2p localize to the daughter side of the bud neck, possibly forming a complex, and are involved in the separation of the virgin daughter from the mother cell during mitosis. The formation of properly assembled birth scars that delimitate cell wall area restricted in the next budding is dependent on the presence of Aim44p and its transcriptional regulator, Swi5p. AIM44 or SWI5 deletion caused the "budding within the birth scar" phenotype, together with altered localization of the birth scar proteins Sun4p and Dse2p, indicating the impairment of birth scar protein complexes.
authors.affiliation: a Department of Genetics and Microbiology, Faculty of Science , Charles University in Prague , Prague , Czech Republic.
authors.forename: Evgeny
authors.initials: E
authors.lastname: Kuznetsov
authors.affiliation: b Institute of Microbiology of the Czech Academy of Sciences, v.v.i. , Prague , Czech Republic.
authors.forename: Libuše
authors.initials: L
authors.lastname: Váchová
authors.affiliation: a Department of Genetics and Microbiology, Faculty of Science , Charles University in Prague , Prague , Czech Republic.
authors.forename: Zdena
authors.initials: Z
authors.lastname: Palková
mesh_terms: D002473:Cell Wall / Q000378:metabolism; D055786:Gene Knockout Techniques; D005959:Glucosidases / Q000378:metabolism*; D010641:Phenotype; D011485:Protein Binding; D021381:Protein Transport; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D005959:Glucosidases; C000588812:Sun4 protein, S cerevisiae
keywords: SUN family of proteins; cell wall; glucanases; yeast birth scar