title: Optimization of Hyaluronidase Inhibition Activity from Prunus davidiana (Carriere) Franch Fruit Extract Fermented by its Isolated Bacillus subtilis Strain SPF4211.
abstract: Strain SPF4211, having hyaluronidase (HAase) inhibition activity, was isolated from P. davidiana (Carriere) Franch fruit (PrDF) sugar extract. The phenotypic and biochemical properties based on 16S rDNA sequencing and an API 50 CHB kit suggested that the organism was B. subtilis. To optimize the HAase inhibition activity of PrDF extract by fermentation of strain SPF4211, a central composite design (CCD) was introduced based on three variables: concentration of PrDF extract (X₁: 1-5%), amount of starter culture (X₂: 1-5%), and fermentation time (X₃: 0-7 days). The experimental data were fitted with quadratic regression equations, and the accuracy of the equations was analyzed by ANOVA. The statistical model predicted the highest HAase inhibition activity of 37.936% under the optimal conditions of X₁ = 1%, X₂ = 2.53%, and X₃ = 7 days. The optimized conditions were validated by observation of an actual HAase inhibition activity of 38.367% from extract of PrDF fermented by SPF4211. These results agree well with the predicted model value.
authors.affiliation: Department of Food Science and Nutrition, College of Human Ecology, Pusan National University, Busan 46241, Republic of Korea.
authors.forename: Won-Baek
authors.initials: WB
authors.lastname: Kim
authors.affiliation: Department of Food Science and Nutrition, College of Human Ecology, Pusan National University, Busan 46241, Republic of Korea.
authors.forename: So Hae
authors.initials: SH
authors.lastname: Park
authors.affiliation: Department of Food Science and Nutrition, College of Human Ecology, Pusan National University, Busan 46241, Republic of Korea.
authors.forename: Kyoung Yoon
authors.initials: KY
authors.lastname: Koo
authors.affiliation: Department of Food Science and Nutrition, College of Human Ecology, Pusan National University, Busan 46241, Republic of Korea.
authors.forename: Bo Ram
authors.initials: BR
authors.lastname: Kim
authors.affiliation: Department of Food Science and Nutrition, College of Human Ecology, Pusan National University, Busan 46241, Republic of Korea.
authors.forename: Minji
authors.initials: M
authors.lastname: Kim
authors.affiliation: Department of Food Science and Nutrition, College of Human Ecology, Pusan National University, Busan 46241, Republic of Korea.
authors.forename: Heeseob
authors.initials: H
authors.lastname: Lee
mesh_terms: D001412:Bacillus subtilis / Q000378:metabolism*; D005285:Fermentation; D005638:Fruit / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism / Q000382:microbiology; D006821:Hyaluronoglucosaminidase / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology*; D027861:Prunus / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism / Q000382:microbiology
chemical_list: D010936:Plant Extracts; D006821:Hyaluronoglucosaminidase
keywords: HAase inhibition activity; P. davidiana (Carriere) Franch fruit; optimization; response surface methodology