title: BM-MSCs and Bio-Oss complexes enhanced new bone formation during maxillary sinus floor augmentation by promoting differentiation of BM-MSCs.
abstract: Bone marrow-derived mesenchymal stem cells (BM-MSCs) have been recognized as a new strategy for maxillary sinus floor elevation. However, little is known concerning the effect of the biomechanical pressure (i.e., sinus pressure, masticatory pressure, and respiration) on the differentiation of BM-MSCs and the formation of new bone during maxillary sinus floor elevation. The differentiation of BM-MSCs into osteoblasts was examined in vitro under cyclic compressive pressure using the Flexcell® pressure system, and by immunohistochemical analysis, qRT-PCR, and Western blot. Micro-CT was used to detect bone formation and allow image reconstruction of the entire maxillary sinus floor elevation area. Differentiation of BM-MSCs into osteoblasts was significantly increased under cyclic compressive pressure. The formation of new bone was enhanced after implantation of the pressured complex of BM-MSCs and Bio-Oss during maxillary sinus floor elevation. The pressured complex of BM-MSCs and Bio-Oss promoted new bone formation and maturation in the rabbit maxillary sinus. Stem cell therapy combined with this tissue engineering technique could be effectively used in maxillary sinus elevation and bone regeneration.
authors.affiliation: Department of Implant Dentistry, The Affiliated Stomatology Hospital of Tongji University, 399 Yanchang Road, Shanghai, 200092, People's Republic of China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Department of Implant Dentistry, The Affiliated Stomatology Hospital of Tongji University, 399 Yanchang Road, Shanghai, 200092, People's Republic of China.
authors.forename: Bo-Han
authors.initials: BH
authors.lastname: Yu
authors.affiliation: Department of Prosthodontics, The Affiliated Stomatology Hospital of Tongji University, Shanghai, 200092, People's Republic of China.
authors.forename: Wei-Cai
authors.initials: WC
authors.lastname: Liu
authors.affiliation: Department of Implant Dentistry, The Affiliated Stomatology Hospital of Tongji University, 399 Yanchang Road, Shanghai, 200092, People's Republic of China. zuolin20131115@163.com.
authors.forename: Zuo-Lin
authors.initials: ZL
authors.lastname: Wang
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation / Q000235:genetics; D006801:Humans; D008443:Maxillary Sinus / Q000187:drug effects / Q000254:growth & development*; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells; D008903:Minerals / Q000008:administration & dosage; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects; D010012:Osteogenesis; D011817:Rabbits; D059546:Sinus Floor Augmentation / Q000379:methods
chemical_list: C077540:Bio-Oss; D008903:Minerals
keywords: Bio-Oss; Biomechanics; Bone marrow-derived mesenchymal stem cells; Maxillary sinus floor augmentation; Pressure