title: Different methods of dentin processing for application in bone tissue engineering: A systematic review.
abstract: Dentin has become an interesting potential biomaterial for tissue engineering of oral hard tissues. It can be used as a scaffold or as a source of growth factors in bone tissue engineering. Different forms of dentin have been studied for their potential use as bone substitutes. Here, we systematically review different methods of dentin preparation and the efficacy of processed dentin in bone tissue engineering. An electronic search was carried out in PubMed and Scopus databases for articles published from 2000 to 2016. Studies on dentin preparation for application in bone tissue engineering were selected. The initial search yielded a total of 1045 articles, of which 37 were finally selected. Review of studies showed that demineralization was the most commonly used dentin preparation process for use in tissue engineering. Dentin extract, dentin particles (tooth ash), freeze-dried dentin, and denatured dentin are others method of dentin preparation. Based on our literature review, we can conclude that preparation procedure and the size and shape of dentin particles play an important role in its osteoinductive and osteoconductive properties. Standardization of these methods is important to draw a conclusion in this regard. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2616-2627, 2016.
authors.affiliation: Department of Dental Biomaterials, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.|Department of Tissue Engineering, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Fahimeh Sadat
authors.initials: FS
authors.lastname: Tabatabaei
authors.affiliation: Department of Dental Biomaterials, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Saeed
authors.initials: S
authors.lastname: Tatari
authors.affiliation: Department of Dental Biomaterials, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Ramin
authors.initials: R
authors.lastname: Samadi
authors.affiliation: School of Clinical Dentistry, University of Sheffield, Claremont Crescent, Sheffield, S10 2TA, United Kingdom.
authors.forename: Keyvan
authors.initials: K
authors.lastname: Moharamzadeh
mesh_terms: D000818:Animals; D018786:Bone Substitutes / Q000737:chemistry* / Q000494:pharmacology*; D003804:Dentin / Q000737:chemistry*; D006801:Humans; D010012:Osteogenesis / Q000187:drug effects*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D018786:Bone Substitutes
keywords: biomaterial; bone substitute; dentin; regeneration; tissue engineering