title: Association of the -243A>G, +61450C>A Polymorphisms of the Glutamate Decarboxylase 2 (GAD2) Gene with Obesity and Insulin Level in North Indian Population.
abstract: BACKGROUND
  Obesity associated with type 2 diabetes, and hypertension increased mortality and morbidity. Glutamate decarboxylase 2 (GAD2) gene is associated with obesity and it regulate food intake and insulin level. We investigated the association of GAD-2gene -243A>G (rs2236418) and +61450C>A (rs992990) polymorphisms with obesity and related phenotypes.
  
  
  METHODS
  Insulin, glucose and lipid levels were estimated using standard protocols. All subjects were genotyped (PCR-RFLP) method.
  
  
  RESULTS
  The -243A>G polymorphism of the GAD-2 gene was significantly associated with higher risk of obesity (P<0.05).
  
  
  CONCLUSIONS
  GAD-2 gene polymorphisms influence obesity and related phenotype in complex manner, probably by regulating the food intake, insulin and body weight.
authors.affiliation: Dept. Physiology, King George's Medical University, Lucknow, India; Dept. of Pediatrics, King George's Medical University, Lucknow, India.
authors.forename: Jai
authors.initials: J
authors.lastname: Prakash
authors.affiliation: Dept. of Genetics, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, India.
authors.forename: Balraj
authors.initials: B
authors.lastname: Mittal
authors.affiliation: Dept. of Pediatrics, King George's Medical University, Lucknow, India.
authors.forename: Shally
authors.initials: S
authors.lastname: Awasthi
authors.affiliation: Dept. Physiology, King George's Medical University, Lucknow, India.
authors.forename: Neena
authors.initials: N
authors.lastname: Srivastava
keywords: Food intake; GAD-2; Insulin; Obesity; Polymorphism