title: A nonsense mutation of Stim1 identified in stroke-prone spontaneously hypertensive rats decreased the store-operated calcium entry in astrocytes.
abstract: We previously identified a nonsense mutation in the stromal interaction molecule-1 (Stim1) resulting in expression of a truncated STIM1 in the stroke-prone spontaneously hypertensive rat (SHRSP). In this study, we evaluated activity of the store-operated Ca(2+)-entry (SOCE) regulated by STIM1 to clarify putative functional abnormalities of the truncated STIM1. As a result, reduced SOCE activity resulting in suppression of cyclooxygenase-2 expression induced by SOCE was found in cultured astrocytes with the truncated STIM1 when compared with those with the wild-type. Our results indicated that the truncated STIM1 impaired Ca(2+) signaling regulated by SOCE and that the impaired SOCE activity might be responsible for pathological phenotypes in SHRSP.
authors.affiliation: Department of Functional Pathology, Shimane University Faculty of Medicine, 89-1 Enya-cho, Izumo, Shimane 693-8501, Japan. Electronic address: oharah@med.shimane-u.ac.jp.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Ohara
authors.affiliation: Department of Functional Pathology, Shimane University Faculty of Medicine, 89-1 Enya-cho, Izumo, Shimane 693-8501, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Nabika
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism* / Q000473:pathology; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D018389:Codon, Nonsense; D051546:Cyclooxygenase 2 / Q000378:metabolism; D005786:Gene Expression Regulation; D055786:Gene Knockout Techniques; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D020521:Stroke / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D000071737:Stromal Interaction Molecule 1 / Q000235:genetics* / Q000378:metabolism
chemical_list: D018389:Codon, Nonsense; C518857:Stim1 protein, rat; D000071737:Stromal Interaction Molecule 1; D051546:Cyclooxygenase 2; C489691:Ptgs2 protein, rat; D002118:Calcium
keywords: Store-operated Ca(2+)-entry; Stroke-prone spontaneously hypertensive rat; Stromal interaction molecule-1