title: Topical MMP beacon enabled fluorescence-guided resection of oral carcinoma.
abstract: Each year almost 300,000 individuals worldwide are diagnosed with oral cancer, more than 90% of these being oral carcinoma [N. Engl. J. Med.328, 1841993]. Surgical resection is the standard of care, but accurate delineation of the tumor boundaries is challenging, resulting in either under-resection with risk of local recurrence or over-resection with increased functional loss and negative impact on quality of life. This study evaluates, in two pre-clinical in vivo tumor models, the potential of fluorescence-guided resection using molecular beacons activated by metalloproteinases, which are frequently upregulated in human oral cancer. In both models there was rapid (<15 min) beacon activation upon local application, allowing clear fluoresecence imaging in vivo and confirmed by ex vivo fluorescence microscopy and HPLC, with minimal activation in normal oral tissues. Although the tissue penetration was limited using topical application, these findings support further development of this approach towards translation to first-in-human trials.
authors.affiliation: Princess Margaret Cancer Centre, University Health Network, 101 College Street, Toronto ON M5G 1L7, Canada; Department of Medical Biophysics, University of Toronto, 101 College Street, Toronto ON M5G 1L7, Canada.
authors.forename: Laura
authors.initials: L
authors.lastname: Burgess
authors.affiliation: Princess Margaret Cancer Centre, University Health Network, 101 College Street, Toronto ON M5G 1L7, Canada.
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Otolaryngology - Head & Neck Surgery, University of Toronto, 190 Elizabeth St, Rm 3S438, RFE Building Toronto, ON, M5G 2N2, Canada.
authors.forename: Nikolaus E
authors.initials: NE
authors.lastname: Wolter
authors.affiliation: Princess Margaret Cancer Centre, University Health Network, 101 College Street, Toronto ON M5G 1L7, Canada; Department of Medical Biophysics, University of Toronto, 101 College Street, Toronto ON M5G 1L7, Canada.
authors.forename: Brian
authors.initials: B
authors.lastname: Wilson
authors.affiliation: Princess Margaret Cancer Centre, University Health Network, 101 College Street, Toronto ON M5G 1L7, Canada; Department of Medical Biophysics, University of Toronto, 101 College Street, Toronto ON M5G 1L7, Canada.
authors.forename: Gang
authors.initials: G
authors.lastname: Zheng
keywords: (110.0110) Imaging systems; (170.0170) Medical optics and biotechnology