title: Retention of Ancestral Genetic Variation Across Life-Stages of an Endangered, Long-Lived Iteroparous Fish.
abstract: As with many endangered, long-lived iteroparous fishes, survival of razorback sucker depends on a management strategy that circumvents recruitment failure that results from predation by non-native fishes. In Lake Mohave, AZ-NV, management of razorback sucker centers on capture of larvae spawned in the lake, rearing them in off-channel habitats, and subsequent release ("repatriation") to the lake when adults are sufficiently large to resist predation. The effects of this strategy on genetic diversity, however, remained uncertain. After correction for differences in sample size among groups, metrics of mitochondrial DNA (mtDNA; number of haplotypes, N H , and haplotype diversity, H D ) and microsatellite (number of alleles, N A , and expected heterozygosity, H E ) diversity did not differ significantly between annual samples of repatriated adults and larval year-classes or among pooled samples of repatriated adults, larvae, and wild fish. These findings indicate that the current management program thus far maintained historical genetic variation of razorback sucker in the lake. Because effective population size, N e , is closely tied to the small census population size (N c = ~1500-3000) of razorback sucker in Lake Mohave, this population will remain at risk from genetic, as well as demographic risk of extinction unless N c is increased substantially.
authors.affiliation: From the Biology Department and Museum of Southwestern Biology, University of New Mexico, MSC03 2020, 1 University of New Mexico Way, Albuquerque, NM 87131-0001 (Carson, Pilger, and Turner); School of Life Sciences, PO Box 874501, Arizona State University, Tempe, AZ 85287-4501 (Adams, Dowling, Marsh, and Saltzgiver); and Marsh & Associates, LLC, Tempe, AZ 85282-6845 (Kesner and Marsh). Dowling and Saltzgiver is now at the Department of Biological Sciences, Wayne State University, Detroit, MI 48202-3917. evan.carson@gmail.com.
authors.forename: Evan W
authors.initials: EW
authors.lastname: Carson
authors.affiliation: From the Biology Department and Museum of Southwestern Biology, University of New Mexico, MSC03 2020, 1 University of New Mexico Way, Albuquerque, NM 87131-0001 (Carson, Pilger, and Turner); School of Life Sciences, PO Box 874501, Arizona State University, Tempe, AZ 85287-4501 (Adams, Dowling, Marsh, and Saltzgiver); and Marsh & Associates, LLC, Tempe, AZ 85282-6845 (Kesner and Marsh). Dowling and Saltzgiver is now at the Department of Biological Sciences, Wayne State University, Detroit, MI 48202-3917.
authors.forename: Thomas F
authors.initials: TF
authors.lastname: Turner
authors.affiliation: From the Biology Department and Museum of Southwestern Biology, University of New Mexico, MSC03 2020, 1 University of New Mexico Way, Albuquerque, NM 87131-0001 (Carson, Pilger, and Turner); School of Life Sciences, PO Box 874501, Arizona State University, Tempe, AZ 85287-4501 (Adams, Dowling, Marsh, and Saltzgiver); and Marsh & Associates, LLC, Tempe, AZ 85282-6845 (Kesner and Marsh). Dowling and Saltzgiver is now at the Department of Biological Sciences, Wayne State University, Detroit, MI 48202-3917.
authors.forename: Melody J
authors.initials: MJ
authors.lastname: Saltzgiver
authors.affiliation: From the Biology Department and Museum of Southwestern Biology, University of New Mexico, MSC03 2020, 1 University of New Mexico Way, Albuquerque, NM 87131-0001 (Carson, Pilger, and Turner); School of Life Sciences, PO Box 874501, Arizona State University, Tempe, AZ 85287-4501 (Adams, Dowling, Marsh, and Saltzgiver); and Marsh & Associates, LLC, Tempe, AZ 85282-6845 (Kesner and Marsh). Dowling and Saltzgiver is now at the Department of Biological Sciences, Wayne State University, Detroit, MI 48202-3917.
authors.forename: Deborah
authors.initials: D
authors.lastname: Adams
authors.affiliation: From the Biology Department and Museum of Southwestern Biology, University of New Mexico, MSC03 2020, 1 University of New Mexico Way, Albuquerque, NM 87131-0001 (Carson, Pilger, and Turner); School of Life Sciences, PO Box 874501, Arizona State University, Tempe, AZ 85287-4501 (Adams, Dowling, Marsh, and Saltzgiver); and Marsh & Associates, LLC, Tempe, AZ 85282-6845 (Kesner and Marsh). Dowling and Saltzgiver is now at the Department of Biological Sciences, Wayne State University, Detroit, MI 48202-3917.
authors.forename: Brian R
authors.initials: BR
authors.lastname: Kesner
authors.affiliation: From the Biology Department and Museum of Southwestern Biology, University of New Mexico, MSC03 2020, 1 University of New Mexico Way, Albuquerque, NM 87131-0001 (Carson, Pilger, and Turner); School of Life Sciences, PO Box 874501, Arizona State University, Tempe, AZ 85287-4501 (Adams, Dowling, Marsh, and Saltzgiver); and Marsh & Associates, LLC, Tempe, AZ 85282-6845 (Kesner and Marsh). Dowling and Saltzgiver is now at the Department of Biological Sciences, Wayne State University, Detroit, MI 48202-3917.
authors.forename: Paul C
authors.initials: PC
authors.lastname: Marsh
authors.affiliation: From the Biology Department and Museum of Southwestern Biology, University of New Mexico, MSC03 2020, 1 University of New Mexico Way, Albuquerque, NM 87131-0001 (Carson, Pilger, and Turner); School of Life Sciences, PO Box 874501, Arizona State University, Tempe, AZ 85287-4501 (Adams, Dowling, Marsh, and Saltzgiver); and Marsh & Associates, LLC, Tempe, AZ 85282-6845 (Kesner and Marsh). Dowling and Saltzgiver is now at the Department of Biological Sciences, Wayne State University, Detroit, MI 48202-3917.
authors.forename: Tyler J
authors.initials: TJ
authors.lastname: Pilger
authors.affiliation: From the Biology Department and Museum of Southwestern Biology, University of New Mexico, MSC03 2020, 1 University of New Mexico Way, Albuquerque, NM 87131-0001 (Carson, Pilger, and Turner); School of Life Sciences, PO Box 874501, Arizona State University, Tempe, AZ 85287-4501 (Adams, Dowling, Marsh, and Saltzgiver); and Marsh & Associates, LLC, Tempe, AZ 85282-6845 (Kesner and Marsh). Dowling and Saltzgiver is now at the Department of Biological Sciences, Wayne State University, Detroit, MI 48202-3917.
authors.forename: Thomas E
authors.initials: TE
authors.lastname: Dowling
mesh_terms: D000483:Alleles; D000818:Animals; D004272:DNA, Mitochondrial; D056727:Endangered Species; D005399:Fishes / Q000235:genetics*; D014644:Genetic Variation; D005828:Genetics, Population; D018895:Microsatellite Repeats
chemical_list: D004272:DNA, Mitochondrial
keywords: census population size; effective number of breeders; effective population size; genetic diversity; microsatellites; mtDNA