title: Engineering Paper-Based Sensors for Zika Virus.
abstract: The emergence of Zika virus (ZIKV) infections in Latin America and Southeast Asia has created an urgent need for new, simple, yet sensitive, diagnostic tests. We highlight recent work using paper-based sensors coupled with CRISPR/Cas9 to detect ZIKV RNA as a new approach to achieve rapid development and deployment of field-ready diagnostics for emerging infectious diseases.
authors.affiliation: Sandia National Laboratories, Biotechnology and Bioengineering Department, Livermore, CA 94551, USA. Electronic address: rmeaghe@sandia.gov.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Meagher
authors.affiliation: Sandia National Laboratories, Biotechnology and Bioengineering Department, Livermore, CA 94551, USA.
authors.forename: Oscar A
authors.initials: OA
authors.lastname: Negrete
authors.affiliation: California National Primate Research Center, University of California, Davis, CA 95616, USA.
authors.forename: Koen K
authors.initials: KK
authors.lastname: Van Rompay
mesh_terms: D064113:CRISPR-Cas Systems; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D010209:Paper; D012367:RNA, Viral / Q000235:genetics* / Q000302:isolation & purification; D000071244:Zika Virus / Q000235:genetics* / Q000302:isolation & purification*; D000071243:Zika Virus Infection / Q000175:diagnosis*
chemical_list: D012367:RNA, Viral
keywords: CRISPR/Cas9; emerging infectious diseases; molecular diagnostics; paper microfluidics; toehold sensors