title: miRNA expression profiling of Epstein-Barr virus-associated NKTL cell lines by Illumina deep sequencing.
abstract: The aim of this work was to establish the microRNA profile of SNK6 and SNT16, two Epstein-Barr virus (EBV)-infected cell lines derived from nasal NK/T-cell lymphoma (NKTL). The oncogenic EBV is strongly associated with the pathogenesis of nasal and extranodal NK/T-cell lymphoma and expresses 44 mature microRNAs and two noncoding EBV-encoded RNAs (EBERs). miRNAs are 19-25nt noncoding RNAs that affect host and viral gene expression post-transcriptionally. Deregulated miRNA patterns are frequently linked to a variety of human cancers including lymphomas. miRNA profiling of the two NK/T cell lines vs. primary cells revealed 10 and 4 up-regulated and 10 and 12 down-regulated miRNAs in SNK6 and SNT16 cells respectively. The results were validated by qRT-PCR for selected miRNAs. Target gene analyses confirmed cullin 5 (CUL5) and sphingosin-1-phosphate receptor 1 (S1PR1) as targets for the down-regulated hsa-miR-148a and viral ebv-miR-BART16 respectively. As recently demonstrated for the regulation of IL1-alpha by miR-142-3p, coexpression of the EBERs selectively exerted corepression of S1PR1 by BART16 but not of CUL5 by miR-148a, indicating selective corepression by the EBERs.
authors.affiliation: Institute of Virology Saarland University Medical School Homburg/Saar Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Alles
authors.affiliation: Institute of Virology Saarland University Medical School Homburg/Saar Germany.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Menegatti
authors.affiliation: Institute of Virology Saarland University Medical School Homburg/Saar Germany; Present address: Boehringer Ingelheim Birkendorfer Strasse 65D-88397 Biberach Germany.
authors.forename: Natalie
authors.initials: N
authors.lastname: Motsch
authors.affiliation: Institute of Virology Saarland University Medical School Homburg/Saar Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Hart
authors.affiliation: Biochemistry Center Regensburg (BZR) Laboratory for RNA Biology University of Regensburg Regensburg Germany.
authors.forename: Norbert
authors.initials: N
authors.lastname: Eichner
authors.affiliation: Max Planck Genome Centre Cologne Cologne Germany.
authors.forename: Richard
authors.initials: R
authors.lastname: Reinhardt
authors.affiliation: Biochemistry Center Regensburg (BZR) Laboratory for RNA Biology University of Regensburg Regensburg Germany.
authors.forename: Gunter
authors.initials: G
authors.lastname: Meister
authors.affiliation: Institute of Virology Saarland University Medical School Homburg/Saar Germany.
authors.forename: Friedrich A
authors.initials: FA
authors.lastname: Grässer
keywords: CUL5; Epstein‐Barr virus; S1PR1; ebv‐miR‐BART16; hsa‐miR‐148a; microRNA