title: High performance CCD camera system for digitalisation of 2D DIGE gels.
abstract: An essential step in 2D DIGE-based analysis of differential proteome profiles is the accurate and sensitive digitalisation of 2D DIGE gels. The performance progress of commercially available charge-coupled device (CCD) camera-based systems combined with light emitting diodes (LED) opens up a new possibility for this type of digitalisation. Here, we assessed the performance of a CCD camera system (Intas Advanced 2D Imager) as alternative to a traditionally employed, high-end laser scanner system (Typhoon 9400) for digitalisation of differential protein profiles from three different environmental bacteria. Overall, the performance of the CCD camera system was comparable to the laser scanner, as evident from very similar protein abundance changes (irrespective of spot position and volume), as well as from linear range and limit of detection.
authors.affiliation: General and Molecular Microbiology, Institute for Chemistry and Biology of the Marine Environment (ICBM), Carl von Ossietzky University Oldenburg, Oldenburg, Germany.|Department of Microbiology, Max Planck Institute for Marine Microbiology, Bremen, Germany.
authors.forename: Annemieke
authors.initials: A
authors.lastname: Strijkstra
authors.affiliation: General and Molecular Microbiology, Institute for Chemistry and Biology of the Marine Environment (ICBM), Carl von Ossietzky University Oldenburg, Oldenburg, Germany.|Department of Microbiology, Max Planck Institute for Marine Microbiology, Bremen, Germany.
authors.forename: Kathleen
authors.initials: K
authors.lastname: Trautwein
authors.affiliation: Intas Science Imaging Instruments GmbH, Göttingen, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Roesler
authors.affiliation: Mathematical Modelling, Institute for Chemistry and Biology of the Marine Environment (ICBM), Carl von Ossietzky University Oldenburg, Oldenburg, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Feenders
authors.affiliation: Intas Science Imaging Instruments GmbH, Göttingen, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Danzer
authors.affiliation: Intas Science Imaging Instruments GmbH, Göttingen, Germany.
authors.forename: Udo
authors.initials: U
authors.lastname: Riemenschneider
authors.affiliation: Mathematical Modelling, Institute for Chemistry and Biology of the Marine Environment (ICBM), Carl von Ossietzky University Oldenburg, Oldenburg, Germany.
authors.forename: Bernd
authors.initials: B
authors.lastname: Blasius
authors.affiliation: General and Molecular Microbiology, Institute for Chemistry and Biology of the Marine Environment (ICBM), Carl von Ossietzky University Oldenburg, Oldenburg, Germany.|Department of Microbiology, Max Planck Institute for Marine Microbiology, Bremen, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Rabus
mesh_terms: D000703:Analog-Digital Conversion; D001426:Bacterial Proteins / Q000302:isolation & purification*; D002232:Carbocyanines / Q000737:chemistry; D020564:Deltaproteobacteria / Q000737:chemistry; D054023:Lasers, Semiconductor; D057230:Limit of Detection; D055096:Optical Devices / Q000592:standards*; D041902:Rhodobacteraceae / Q000737:chemistry; D043362:Rhodocyclaceae / Q000737:chemistry; D057825:Two-Dimensional Difference Gel Electrophoresis / Q000295:instrumentation*
chemical_list: D001426:Bacterial Proteins; D002232:Carbocyanines
keywords: 2D DIGE; CCD camera; Intas Advanced 2D Imager; Laser scanner; Technology; Typhoon