title: Genome sequencing and annotation of a Campylobacter coli strain isolated from milk with multidrug resistance.
abstract: As the most prevalent bacterial cause of human gastroenteritis, food-borne Campylobacter infections pose a serious threat to public health. Whole Genome Sequencing (WGS) is a tool providing quick and inexpensive approaches for analysis of food-borne pathogen epidemics. Here we report the WGS and annotation of a Campylobacter coli strain, FNW20G12, which was isolated from milk in the United States in 1997 and carries multidrug resistance. The draft genome of FNW20G12 (DDBJ/ENA/GenBank accession number LWIH00000000) contains 1, 855,435 bp (GC content 31.4%) with 1902 annotated coding regions, 48 RNAs and resistance to aminoglycoside, beta-lactams, tetracycline, as well as fluoroquinolones. There are very few genome reports of C. coli from dairy products with multidrug resistance. Here the draft genome of FNW20G12, a C. coli strain isolated from raw milk, is presented to aid in the epidemiology study of C. coli antimicrobial resistance and role in foodborne outbreak.
authors.affiliation: Applied Technology Center, Pacific Regional Laboratory Northwest, US Food and Drug Administration, 22201 23rd Drive SE, Bothell, WA 98012, United States.
authors.forename: Kun C
authors.initials: KC
authors.lastname: Liu
authors.affiliation: Applied Technology Center, Pacific Regional Laboratory Northwest, US Food and Drug Administration, 22201 23rd Drive SE, Bothell, WA 98012, United States.
authors.forename: Karen C
authors.initials: KC
authors.lastname: Jinneman
authors.affiliation: Applied Technology Center, Pacific Regional Laboratory Northwest, US Food and Drug Administration, 22201 23rd Drive SE, Bothell, WA 98012, United States.
authors.forename: Jason
authors.initials: J
authors.lastname: Neal-McKinney
authors.affiliation: Applied Technology Center, Pacific Regional Laboratory Northwest, US Food and Drug Administration, 22201 23rd Drive SE, Bothell, WA 98012, United States.
authors.forename: Wen-Hsin
authors.initials: WH
authors.lastname: Wu
authors.affiliation: Applied Technology Center, Pacific Regional Laboratory Northwest, US Food and Drug Administration, 22201 23rd Drive SE, Bothell, WA 98012, United States.
authors.forename: Daniel H
authors.initials: DH
authors.lastname: Rice
keywords: C. coli (Campylobacter coli); RAST (Rapid Annotation using Subsystem Technology); WGS (Whole Genome Sequencing)