title: Cyclical DNA Methylation and Histone Changes Are Induced by LPS to Activate COX-2 in Human Intestinal Epithelial Cells.
abstract: Bacterial lipopolysaccharide (LPS) induces release of inflammatory mediators both in immune and epithelial cells. We investigated whether changes of epigenetic marks, including selected histone modification and DNA methylation, may drive or accompany the activation of COX-2 gene in HT-29 human intestinal epithelial cells upon exposure to LPS. Here we describe cyclical histone acetylation (H3), methylation (H3K4, H3K9, H3K27) and DNA methylation changes occurring at COX-2 gene promoter overtime after LPS stimulation. Histone K27 methylation changes are carried out by the H3 demethylase JMJD3 and are essential for COX-2 induction by LPS. The changes of the histone code are associated with cyclical methylation signatures at the promoter and gene body of COX-2 gene.
authors.affiliation: Dipartimento di Biologia, Università degli Studi di Napoli "Federico II", Monte Sant'Angelo via Cintia 21, 80126 Naples, Italy.
authors.forename: Tiziana
authors.initials: T
authors.lastname: Angrisano
authors.affiliation: Dipartimento di Medicina Molecolare e Biotecnologie Mediche, via S. Pansini, 5, 80131 Naples, Italy.|Istituto di Endocrinologia ed Oncologia Sperimentale C.N.R., via S. Pansini, 5, 80131 Naples, Italy.
authors.forename: Raffaela
authors.initials: R
authors.lastname: Pero
authors.affiliation: Dipartimento di Medicina Molecolare e Biotecnologie Mediche, via S. Pansini, 5, 80131 Naples, Italy.
authors.forename: Mariarita
authors.initials: M
authors.lastname: Brancaccio
authors.affiliation: Dipartimento di Medicina Molecolare e Biotecnologie Mediche, via S. Pansini, 5, 80131 Naples, Italy.|Istituto di Endocrinologia ed Oncologia Sperimentale C.N.R., via S. Pansini, 5, 80131 Naples, Italy.
authors.forename: Lorena
authors.initials: L
authors.lastname: Coretti
authors.affiliation: Dipartimento di Medicina Molecolare e Biotecnologie Mediche, via S. Pansini, 5, 80131 Naples, Italy.|Istituto di Endocrinologia ed Oncologia Sperimentale C.N.R., via S. Pansini, 5, 80131 Naples, Italy.
authors.forename: Ermanno
authors.initials: E
authors.lastname: Florio
authors.affiliation: Dipartimento di Medicina Molecolare e Biotecnologie Mediche, via S. Pansini, 5, 80131 Naples, Italy.|Istituto di Endocrinologia ed Oncologia Sperimentale C.N.R., via S. Pansini, 5, 80131 Naples, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Pezone
authors.affiliation: Dipartimento di Biologia, Università degli Studi di Napoli "Federico II", Monte Sant'Angelo via Cintia 21, 80126 Naples, Italy.
authors.forename: Viola
authors.initials: V
authors.lastname: Calabrò
authors.affiliation: Dipartimento di Biologia, Università degli Studi di Napoli "Federico II", Monte Sant'Angelo via Cintia 21, 80126 Naples, Italy.
authors.forename: Geppino
authors.initials: G
authors.lastname: Falco
authors.affiliation: Dipartimento di Medicina Molecolare e Biotecnologie Mediche, via S. Pansini, 5, 80131 Naples, Italy.|Istituto di Endocrinologia ed Oncologia Sperimentale C.N.R., via S. Pansini, 5, 80131 Naples, Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: Keller
authors.affiliation: Dipartimento di Farmacia, Università degli Studi di Napoli "Federico II", via D. Montesano 47, 80131 Naples, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Lembo
authors.affiliation: Dipartimento di Medicina Molecolare e Biotecnologie Mediche, via S. Pansini, 5, 80131 Naples, Italy.
authors.forename: Vittorio Enrico
authors.initials: VE
authors.lastname: Avvedimento
authors.affiliation: Dipartimento di Medicina Molecolare e Biotecnologie Mediche, via S. Pansini, 5, 80131 Naples, Italy.|Istituto di Endocrinologia ed Oncologia Sperimentale C.N.R., via S. Pansini, 5, 80131 Naples, Italy.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Chiariotti
mesh_terms: D018899:CpG Islands / Q000235:genetics; D051546:Cyclooxygenase 2 / Q000235:genetics* / Q000378:metabolism; D019175:DNA Methylation / Q000235:genetics*; D004789:Enzyme Activation / Q000187:drug effects; D044127:Epigenesis, Genetic / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects / Q000201:enzymology*; D020868:Gene Silencing / Q000187:drug effects; D019073:HT29 Cells; D006657:Histones / Q000378:metabolism*; D006801:Humans; D007422:Intestines / Q000166:cytology*; D056484:Jumonji Domain-Containing Histone Demethylases / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008239:Lysine / Q000378:metabolism; D011401:Promoter Regions, Genetic; D034741:RNA, Small Interfering / Q000378:metabolism
chemical_list: D006657:Histones; D008070:Lipopolysaccharides; D034741:RNA, Small Interfering; D056484:Jumonji Domain-Containing Histone Demethylases; C524220:KDM6B protein, human; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D008239:Lysine