title: [Influence of chronic alcohol treatment on the expression of the Bdnf, Bax, Bcl-xL, and CASP3 genes in the mouse brain: Role of the C1473G polymorphism in the gene encoding tryptophan hydroxylase 2].
abstract: Tryptophan hydroxylase 2 (Tph-2) is the key enzyme in serotonin biosynthesis. Serotonin is one of the main neurotransmitters involved in the regulation of various physiological functions and behavior patterns. The influence of chronic ethanol consumption on the expression of the Bdnf, Bax, Bcl-xL, and CASP3 genes was studied in the brain structures of B6-1473C (C/C) and B6-1473G (G/G) mice that had been obtained on the base of the C57BL/6 strain. The strains differed in the genotype for the C1473G single nucleotide polymorphism in the Tph-2 gene and in Tph-2 enzyme activity. It was found that chronic alcohol treatment led to a significant increase in the expression of the Bdnf gene in the midbrain of B6-1473G mice, but not in B6-1473С. Chronic alcohol treatment considerably decreased the expression of the ultimate brain apoptosis effector, caspase 3, in the frontal cortex, but increased it in the hippocampus of B6-1473G mice. At the same time, chronic ethanol administration reduced the level of the antiapoptotic Bcl-xL mRNA in the midbrain of B6-1473C mice. Thus, the C1473G polymorphism in the Tph-2 gene considerably influenced the changes in the expression patterns of genes involved in the regulation of neurogenesis and neural apoptosis induced by chronic ethanol treatment.
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia.
authors.forename: D V
authors.initials: DV
authors.lastname: Bazovkina
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia.
authors.forename: A S
authors.initials: AS
authors.lastname: Tsybko
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Filimonova
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia.
authors.forename: T V
authors.initials: TV
authors.lastname: Ilchibaeva
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia.|naumenko2002@bionet.nsc.ru.
authors.forename: V S
authors.initials: VS
authors.lastname: Naumenko
mesh_terms: D000437:Alcoholism / Q000235:genetics* / Q000473:pathology; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis* / Q000235:genetics; D053148:Caspase 3 / Q000096:biosynthesis* / Q000235:genetics; D000431:Ethanol / Q000008:administration & dosage; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D051379:Mice; D012701:Serotonin / Q000096:biosynthesis; D014365:Tryptophan Hydroxylase / Q000096:biosynthesis / Q000235:genetics*; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis* / Q000235:genetics; D051020:bcl-X Protein / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C494827:Bax protein, mouse; C494812:Bcl2l1 protein, mouse; D019208:Brain-Derived Neurotrophic Factor; D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein; D012701:Serotonin; D000431:Ethanol; C487802:Tph2 protein, mouse; D014365:Tryptophan Hydroxylase; D053148:Caspase 3
keywords: Bax; Bcl-xL; Bdnf; C1473G polymorphism; caspase 3; chronic alcohol treatment; gene expression; mice; tryptophan hydroxylase 2