title: [Creation of DNA vaccine vector based on codon-optimized gene of rabies virus glycoprotein (G protein) with consensus amino acid sequence].
abstract: An optimized design of the rabies virus glycoprotein (G protein) for use within DNA vaccines has been suggested. The design represents a territorially adapted antigen constructed taking into account glycoprotein amino acid sequences of the rabies viruses registered in the Russian Federation and the vaccine Vnukovo-32 strain. Based on the created consensus amino acid sequence, the nucleotide codon-optimized sequence of this modified glycoprotein was obtained and cloned into the pVAX1 plasmid (a vector of the last generation used in the creation of DNA vaccines). A twofold increase in this gene expression compared to the expression of the Vnukovo-32 strain viral glycoprotein gene in a similar vector was registered in the transfected cell culture. It has been demonstrated that the accumulation of modified G protein exceeds the number of the control protein synthesized using the plasmid with the Vnukovo-32 strain viral glycoprotein gene by 20 times. Thus, the obtained modified rabies virus glycoprotein can be considered to be a promising DNA vaccine antigen.
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.|estarodubova@yandex.ru.
authors.forename: E S
authors.initials: ES
authors.lastname: Starodubova
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
authors.forename: Y V
authors.initials: YV
authors.lastname: Kuzmenko
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Latanova
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
authors.forename: O V
authors.initials: OV
authors.lastname: Preobrazhenskaya
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
authors.forename: V L
authors.initials: VL
authors.lastname: Karpov
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D003062:Codon; D005822:Genetic Vectors; D006023:Glycoproteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D006367:HeLa Cells; D006801:Humans; D010446:Peptide Fragments / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D011818:Rabies / Q000235:genetics / Q000276:immunology* / Q000517:prevention & control* / Q000821:virology; D019444:Vaccines, DNA / Q000235:genetics* / Q000276:immunology / Q000627:therapeutic use / Q000821:virology; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology
chemical_list: D003062:Codon; D006023:Glycoproteins; D010446:Peptide Fragments; D019444:Vaccines, DNA; D014764:Viral Proteins; C583075:rabies virus glycoprotein peptide
keywords: DNA vaccine; Vnukovo-32; consensus amino acid sequence; glycoprotein; rabies virus