title: Isolation and structure determination of a new lantibiotic cinnamycin B from Actinomadura atramentaria based on genome mining.
abstract: New lantibiotic cinnamycin B was isolated from the extract of Actinomadura atramentaria NBRC 14695(T), based on genome mining and chemical investigation. The partial structure of cinnamycin B was established by 2D NMR experiments, which indicated that cinnamycin B had same methyl lanthionine bridging pattern with cinnamycin. The reduction with NaBH4-NiCl2 afforded the reduced cinnamycin B, and MS/MS experiment indicated the presence of hydroxy asparatic acid in the molecule. Cinnamycin B showed an antibacterial activity against Streptomyces antibioticus with dosage of 5 μg (0.5μL, 10 mg/mL solution) at spot-on-lawn testing method. The gene cluster of cinnamycin B on the genome of A. atramentaria was identified and discussed in comparison with that of cinnamycin.
authors.affiliation: College of Agriculture, Academic Institute, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan. kodani.shinya@shizuoka.ac.jp.|Graduate School of Integrated Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan. kodani.shinya@shizuoka.ac.jp.|Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan. kodani.shinya@shizuoka.ac.jp.
authors.forename: Shinya
authors.initials: S
authors.lastname: Kodani
authors.affiliation: Biological Resource Center, National Institute of Technology and Evaluation (NBRC), 2-5-8 Kazusakamatari, Kisarazu, Chiba, 292‒0818, Japan.
authors.forename: Hisayuki
authors.initials: H
authors.lastname: Komaki
authors.affiliation: Graduate School of Integrated Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan.
authors.forename: Sho
authors.initials: S
authors.lastname: Ishimura
authors.affiliation: Food Research Institute, NARO, 2-1-12 Kan-nondai, Tsukuba, Ibaraki, 305-8642, Japan.
authors.forename: Hikaru
authors.initials: H
authors.lastname: Hemmi
authors.affiliation: Food Research Institute, NARO, 2-1-12 Kan-nondai, Tsukuba, Ibaraki, 305-8642, Japan.
authors.forename: Mayumi
authors.initials: M
authors.lastname: Ohnishi-Kameyama
mesh_terms: D000192:Actinomycetales / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D001430:Bacteriocins / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D057225:Data Mining; D016680:Genome, Bacterial; D023281:Genomics; D010456:Peptides, Cyclic / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D010456:Peptides, Cyclic; C000624456:cinnamycin B; C000485:cinnamycin
keywords: Actinomadura atramentaria NBRC 14695; Genome mining; Lantibiotic; MS/MS analysis; NMR spectrum