title: Nonenzymatic all-solid-state coated wire electrode for acetylcholine determination in vitro.
abstract: A nonenzymatic all-solid-state coated wire acetylcholine electrode was investigated. Poly(3,4-ethylenedioxythiophene) doped with poly(styrenesulfonate) (PEDOT/PSS) as conducting polymer was coated on one end of a gold wire (0.5mm in diameter). The acetylcholine selective membrane containing heptakis(2,3,6-tri-Ο-methyl)-β-cyclodextrin as an ionophore covered the conducting polymer layer. The electrode could work stably in a pH range of 6.5-8.5 and a temperature range of 15-40°C. It covered an acetylcholine concentration range of 10(-5)-10(-1)M with a slope of 54.04±1.70mV/decade, while detection limit was 5.69±1.06µM. The selectivity, dynamic response, reproducibility and stability were evaluated. The electrode could work properly in the rat brain homogenate to detect different concentrations of acetylcholine.
authors.affiliation: State Key Laboratory of Industrial Control Technology, College of Control Science and Engineering, Zhejiang University, Hangzhou 310027, Zhejiang, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: He
authors.affiliation: State Key Laboratory of Industrial Control Technology, College of Control Science and Engineering, Zhejiang University, Hangzhou 310027, Zhejiang, China.
authors.forename: Zhan
authors.initials: Z
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Industrial Control Technology, College of Control Science and Engineering, Zhejiang University, Hangzhou 310027, Zhejiang, China.
authors.forename: You
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Industrial Control Technology, College of Control Science and Engineering, Zhejiang University, Hangzhou 310027, Zhejiang, China.
authors.forename: Ruifen
authors.initials: R
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Industrial Control Technology, College of Control Science and Engineering, Zhejiang University, Hangzhou 310027, Zhejiang, China. Electronic address: guangli@zju.edu.cn.
authors.forename: Guang
authors.initials: G
authors.lastname: Li
mesh_terms: D000109:Acetylcholine / Q000032:analysis*; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation*; D001923:Brain Chemistry; D004553:Electric Conductivity; D004867:Equipment Design; D007476:Ionophores / Q000737:chemistry*; D008839:Microelectrodes; D011137:Polystyrenes / Q000737:chemistry; D011199:Potentiometry / Q000295:instrumentation*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D013876:Thiophenes / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D007476:Ionophores; D011137:Polystyrenes; D013876:Thiophenes; D047392:beta-Cyclodextrins; C533756:poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate); C000609658:heptakis(2,3,6-tri-O-methyl)-beta-cyclodextrin; D000109:Acetylcholine
keywords: Acetylcholine electrode; All-solid-state; Coated wire; Conducting polymer; Rat brain homogenate