title: Primary Air-Liquid Interface Culture of Nasal Epithelium for Nasal Drug Delivery.
abstract: Nasal drug administration is a promising alternative to oral and parenteral administration for both local and systemic delivery of drugs. The benefits include its noninvasive nature, rapid absorption, and circumvention of first pass metabolism. Hence, the use of an in vitro model using human primary nasal epithelial cells could be key to understanding important functions and parameters of the respiratory epithelium. This model will enable investigators to address important and original research questions using a biologically relevant in vitro platform that mimics the in vivo nasal epithelial physiology. The purpose of this study was to establish, systematically characterize, and validate the use of a primary human nasal epithelium model cultured at the air-liquid interface for the study of inflammatory responses and drug transport and to simultaneously quantify drug effects on ciliary activity.
authors.affiliation: Faculty of Health Sciences, Southampton University , Southampton SO16 6YD, U.K.|NIHR Southampton Respiratory Biomedical Research Unit, University of Southampton and University Hospital Southampton NHS Foundation Trust , Southampton SO16 6YD, U.K.|Respiratory Technology, Woolcock Institite of Medical Research , Glebe, New South Wales 2037, Australia.|Discipline of Pharmacology, Sydney Medical School , Sydney, New South Wales 2006, Australia.
authors.forename: Hui Xin
authors.initials: HX
authors.lastname: Ong
authors.affiliation: NIHR Southampton Respiratory Biomedical Research Unit, University of Southampton and University Hospital Southampton NHS Foundation Trust , Southampton SO16 6YD, U.K.|Primary Ciliary Dyskinesia Centre, University Hospital Southampton NHS Foundation Trust , Southampton SO16 6YD, U.K.
authors.forename: Claire L
authors.initials: CL
authors.lastname: Jackson
authors.affiliation: NIHR Southampton Respiratory Biomedical Research Unit, University of Southampton and University Hospital Southampton NHS Foundation Trust , Southampton SO16 6YD, U.K.|Primary Ciliary Dyskinesia Centre, University Hospital Southampton NHS Foundation Trust , Southampton SO16 6YD, U.K.
authors.forename: Janice L
authors.initials: JL
authors.lastname: Cole
authors.affiliation: NIHR Southampton Respiratory Biomedical Research Unit, University of Southampton and University Hospital Southampton NHS Foundation Trust , Southampton SO16 6YD, U.K.|Primary Ciliary Dyskinesia Centre, University Hospital Southampton NHS Foundation Trust , Southampton SO16 6YD, U.K.
authors.forename: Peter M
authors.initials: PM
authors.lastname: Lackie
authors.affiliation: Respiratory Technology, Woolcock Institite of Medical Research , Glebe, New South Wales 2037, Australia.|Discipline of Pharmacology, Sydney Medical School , Sydney, New South Wales 2006, Australia.
authors.forename: Daniela
authors.initials: D
authors.lastname: Traini
authors.affiliation: Respiratory Technology, Woolcock Institite of Medical Research , Glebe, New South Wales 2037, Australia.|Discipline of Pharmacology, Sydney Medical School , Sydney, New South Wales 2006, Australia.
authors.forename: Paul M
authors.initials: PM
authors.lastname: Young
authors.affiliation: NIHR Southampton Respiratory Biomedical Research Unit, University of Southampton and University Hospital Southampton NHS Foundation Trust , Southampton SO16 6YD, U.K.|Primary Ciliary Dyskinesia Centre, University Hospital Southampton NHS Foundation Trust , Southampton SO16 6YD, U.K.
authors.forename: Jane
authors.initials: J
authors.lastname: Lucas
authors.affiliation: Faculty of Health Sciences, Southampton University , Southampton SO16 6YD, U.K.|NIHR Southampton Respiratory Biomedical Research Unit, University of Southampton and University Hospital Southampton NHS Foundation Trust , Southampton SO16 6YD, U.K.
authors.forename: Joy
authors.initials: J
authors.lastname: Conway
mesh_terms: D000281:Administration, Intranasal / Q000379:methods; D000328:Adult; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D016503:Drug Delivery Systems / Q000379:methods; D004847:Epithelial Cells / Q000502:physiology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009297:Nasal Mucosa / Q000502:physiology*; D004364:Pharmaceutical Preparations / Q000008:administration & dosage*; D020545:Respiratory Mucosa / Q000502:physiology*
chemical_list: D004364:Pharmaceutical Preparations
keywords: ciliary function; epithelial cells; nasal drug delivery; tobramycin; transport