title: Downregulation of miR-101 contributes to epithelial-mesenchymal transition in cisplatin resistance of NSCLC cells by targeting ROCK2.
abstract: Chemoresistance and epithelial-mesenchymal transition (EMT) in cancer are linked phenomena. EMT contributes to chemoresistance, however, little is known about whether chemotherapy can induce EMT in cancer cells. Here, we found that miR-101 expression was downregulated in cisplatin-resistant non-small cell lung cancer (NSCLC) cells. Restoration of miR-101 expression inhibited EMT and increased the sensitivity of cisplatin-resistant NSCLC cells to cisplatin in vitro by targeting ROCK2. Furthermore, ROCK2 protein level was inversely correlated with miR-101 level in NSCLC tissue samples. Kaplan-Meier analysis revealed that low miR-101 expression in NSCLC was correlated with poor survival time. In summary, our results provide novel mechanistic insights into the role of miR-101/ROCK2 signaling in the cisplatin resistance of NSCLC cells. Targeting of miR-101 is a potential therapeutic approach for NSCLC.
authors.affiliation: Department of Emergency, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Ye
authors.affiliation: Department of Cardiac Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
authors.forename: Shengli
authors.initials: S
authors.lastname: Yin
authors.affiliation: Department of Ultrasonography, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
authors.forename: Zhongzhen
authors.initials: Z
authors.lastname: Su
authors.affiliation: Department of Radiology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
authors.forename: Mingjun
authors.initials: M
authors.lastname: Bai
authors.affiliation: Department of Medical Research Center, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Anesthesiology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
authors.forename: Ziqing
authors.initials: Z
authors.lastname: Hei
authors.affiliation: Department of Cardiothoracic Surgery, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
authors.forename: Songwang
authors.initials: S
authors.lastname: Cai
mesh_terms: D000072283:A549 Cells; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy* / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology*; D015536:Down-Regulation; D019008:Drug Resistance, Neoplasm; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects*; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D014162:Transfection; D054460:rho-Associated Kinases / Q000235:genetics* / Q000378:metabolism*
chemical_list: C534072:MIRN101 microRNA, human; D035683:MicroRNAs; C515852:ROCK2 protein, human; D054460:rho-Associated Kinases; D002945:Cisplatin
keywords: ROCK2; chemoresistance; epithelial-mesenchymal transition; miR-101; non-small cell lung cancer