title: aPKC regulates apical localization of Lgl to restrict elongation of microridges in developing zebrafish epidermis.
abstract: Epithelial cells exhibit apical membrane protrusions, which confer specific functions to epithelial tissues. Microridges are short actin protrusions that are laterally long and form a maze-like pattern in the apical domain. They are widely found on vertebrate squamous epithelia including epidermis and have functions in mucous retention, membrane storage and abrasion resistance. It is largely unknown how the formation of these laterally long actin projections is regulated. Here, we show that antagonistic interactions between aPKC and Lgl-regulators of apical and basolateral domain identity, respectively,-control the length of microridges in the zebrafish periderm, the outermost layer of the epidermis. aPKC regulates the levels of Lgl and the active form of non-muscle myosinII at the apical cortex to prevent actin polymerization-dependent precocious fusion and elongation of microridges. Our data unravels the functional significance of exclusion of Lgl from the apical domain in epithelial cells.
authors.affiliation: Department of Biological Sciences, Tata Institute of Fundamental Research, Colaba, Mumbai 400005, India.
authors.forename: Renuka
authors.initials: R
authors.lastname: Raman
authors.affiliation: Department of Biological Sciences, Tata Institute of Fundamental Research, Colaba, Mumbai 400005, India.
authors.forename: Indraneel
authors.initials: I
authors.lastname: Damle
authors.affiliation: Department of Biological Sciences, Tata Institute of Fundamental Research, Colaba, Mumbai 400005, India.
authors.forename: Rahul
authors.initials: R
authors.lastname: Rote
authors.affiliation: Department of Biological Sciences, Tata Institute of Fundamental Research, Colaba, Mumbai 400005, India.|SASTRA University, Tirumalaisamudram, Thanjavur 613402, India.
authors.forename: Shamik
authors.initials: S
authors.lastname: Banerjee
authors.affiliation: Department of Biological Sciences, Tata Institute of Fundamental Research, Colaba, Mumbai 400005, India.
authors.forename: Chaitanya
authors.initials: C
authors.lastname: Dingare
authors.affiliation: Department of Biological Sciences, Tata Institute of Fundamental Research, Colaba, Mumbai 400005, India.
authors.forename: Mahendra
authors.initials: M
authors.lastname: Sonawane
mesh_terms: D008841:Actin Cytoskeleton / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D000199:Actins / Q000235:genetics / Q000378:metabolism; D000818:Animals; D030801:Animals, Genetically Modified; D016764:Cell Polarity; D004625:Embryo, Nonmammalian; D047108:Embryonic Development / Q000235:genetics; D000078404:Epidermal Cells; D004817:Epidermis / Q000254:growth & development / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism* / Q000648:ultrastructure; D018507:Gene Expression Regulation, Developmental; D006023:Glycoproteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D060172:Morpholinos / Q000235:genetics / Q000378:metabolism; D024462:Myosin Type II / Q000235:genetics / Q000378:metabolism; D011493:Protein Kinase C / Q000235:genetics / Q000378:metabolism*; D015027:Zebrafish / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029961:Zebrafish Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
chemical_list: D000199:Actins; C581700:CRISPLD2 protein, zebrafish; D006023:Glycoproteins; D060172:Morpholinos; D029961:Zebrafish Proteins; C503593:llgl2 protein, zebrafish; C115015:PKC-3 protein; D011493:Protein Kinase C; D024462:Myosin Type II