title: Alterations of the bone marrow stromal microenvironment in adult patients with acute myeloid and lymphoblastic leukemias before and after allogeneic hematopoietic stem cell transplantation.
abstract: Bone marrow (BM) derived adult multipotent mesenchymal stromal cells (MMSCs) and fibroblast colony-forming units (CFU-Fs) of 20 patients with acute myeloid leukemia (AML) and 15 patients with acute lymphoblastic leukemia (ALL) before and during 1 year after receiving allogeneic hematopoietic stem cell transplantation (allo-HSCT) were studied. The growth characteristics of MMSCs of all patients before allo-HSCT were not altered; however, relative expression level (REL) of some genes in MMSCs, but not in CFU-Fs, from AML and ALL patients significantly changed. After allo-HSCT, CFU-F concentration and MMSC production were significantly decreased for 1 year; REL of several genes in MMSCs and CFU-F-derived colonies were also significantly downregulated. Thus, chemotherapy that was used for induction of remission did not impair the function of stromal precursors, but gene expression levels were altered. Allo-HSCT conditioning regimens significantly damaged MMSCs and CFU-Fs, and the effect lasted for at least 1 year.
authors.affiliation: a Physiology of Hematopoiesis Lab , Federal Government Budget Institution National Research Center for Hematology, Ministry of Health , Moscow , Russian Federation.
authors.forename: Irina N
authors.initials: IN
authors.lastname: Shipounova
authors.affiliation: a Physiology of Hematopoiesis Lab , Federal Government Budget Institution National Research Center for Hematology, Ministry of Health , Moscow , Russian Federation.
authors.forename: Nataliya A
authors.initials: NA
authors.lastname: Petinati
authors.affiliation: a Physiology of Hematopoiesis Lab , Federal Government Budget Institution National Research Center for Hematology, Ministry of Health , Moscow , Russian Federation.
authors.forename: Alexey E
authors.initials: AE
authors.lastname: Bigildeev
authors.affiliation: a Physiology of Hematopoiesis Lab , Federal Government Budget Institution National Research Center for Hematology, Ministry of Health , Moscow , Russian Federation.
authors.forename: Nina J
authors.initials: NJ
authors.lastname: Drize
authors.affiliation: b Department of High-Dose Chemotherapy , Depressions of Hematopoiesis and Bone Marrow Transplantation, Federal Government Budget Institution National Research Center for Hematology, Ministry of Health , Moscow , Russian Federation.
authors.forename: Tamara V
authors.initials: TV
authors.lastname: Sorokina
authors.affiliation: b Department of High-Dose Chemotherapy , Depressions of Hematopoiesis and Bone Marrow Transplantation, Federal Government Budget Institution National Research Center for Hematology, Ministry of Health , Moscow , Russian Federation.
authors.forename: Larisa A
authors.initials: LA
authors.lastname: Kuzmina
authors.affiliation: b Department of High-Dose Chemotherapy , Depressions of Hematopoiesis and Bone Marrow Transplantation, Federal Government Budget Institution National Research Center for Hematology, Ministry of Health , Moscow , Russian Federation.
authors.forename: Elena N
authors.initials: EN
authors.lastname: Parovichnikova
authors.affiliation: c Federal Government Budget Institution National Research Center for Hematology, Ministry of Health , Moscow , Russian Federation.
authors.forename: Valeri G
authors.initials: VG
authors.lastname: Savchenko
mesh_terms: D000293:Adolescent; D000328:Adult; D015415:Biomarkers; D001853:Bone Marrow / Q000378:metabolism / Q000473:pathology*; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D005260:Female; D015870:Gene Expression; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics / Q000473:pathology* / Q000628:therapy; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000473:pathology*; D011184:Postoperative Period; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics / Q000473:pathology* / Q000628:therapy; D057234:Preoperative Period; D013997:Time Factors; D014184:Transplantation, Homologous; D059016:Tumor Microenvironment / Q000235:genetics; D055815:Young Adult
chemical_list: D015415:Biomarkers
keywords: Acute lymphoblastic leukemia; acute myeloid leukemia; allogeneic hematopoietic stem cell transplantation; fibroblast colony forming units; gene expression; multipotent mesenchymal stromal cells