title: Cep78 is a new centriolar protein involved in Plk4-induced centriole overduplication.
abstract: Centrioles are core components of centrosomes, the major microtubule-organizing centers of animal cells, and act as basal bodies for cilia formation. Control of centriole number is therefore crucial for genome stability and embryogenesis. Centriole duplication requires the serine/threonine protein kinase Plk4. Here, we identify Cep78 as a human centrosomal protein and a new interaction partner of Plk4. Cep78 is mainly a centriolar protein that localizes to the centriolar wall. Furthermore, we find that Plk4 binds to Cep78 through its N-terminal domain but that Cep78 is not an in vitro Plk4 substrate. Cep78 colocalizes with Plk4 at centrioles and is required for Plk4-induced centriole overduplication. Interestingly, upon depletion of Cep78, newly synthesized Plk4 is not localized to centrosomes. Our results suggest that the interaction between Cep78 and the N-terminal catalytic domain of Plk4 is a new and important element in the centrosome overduplication process.
authors.affiliation: Cell Cycle Control and Carcinogenesis (F045) German Cancer Research Center, DKFZ, Im Neuenheimer Feld 242, Heidelberg D-69120, Germany.
authors.forename: Kathrin
authors.initials: K
authors.lastname: Brunk
authors.affiliation: Cell Cycle Control and Carcinogenesis (F045) German Cancer Research Center, DKFZ, Im Neuenheimer Feld 242, Heidelberg D-69120, Germany.
authors.forename: Mei
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Cell Cycle Control and Carcinogenesis (F045) German Cancer Research Center, DKFZ, Im Neuenheimer Feld 242, Heidelberg D-69120, Germany.
authors.forename: Felix
authors.initials: F
authors.lastname: Bärenz
authors.affiliation: Cell Cycle Control and Carcinogenesis (F045) German Cancer Research Center, DKFZ, Im Neuenheimer Feld 242, Heidelberg D-69120, Germany.
authors.forename: Anne-Sophie
authors.initials: AS
authors.lastname: Kratz
authors.affiliation: European Molecular Biology Laboratory, Meyerhofstr. 1, Heidelberg D-69117, Germany.
authors.forename: Uta
authors.initials: U
authors.lastname: Haselmann-Weiss
authors.affiliation: European Molecular Biology Laboratory, Meyerhofstr. 1, Heidelberg D-69117, Germany.
authors.forename: Claude
authors.initials: C
authors.lastname: Antony
authors.affiliation: Cell Cycle Control and Carcinogenesis (F045) German Cancer Research Center, DKFZ, Im Neuenheimer Feld 242, Heidelberg D-69120, Germany ingrid.hoffmann@dkfz.de.
authors.forename: Ingrid
authors.identifier: 0000-0002-8894-8597
authors.initials: I
authors.lastname: Hoffmann
mesh_terms: D018797:Cell Cycle Proteins / Q000378:metabolism*; D002502:Centrioles / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D007399:Interphase; D011485:Protein Binding; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D021381:Protein Transport
chemical_list: C000618001:CEP78 protein, human; D018797:Cell Cycle Proteins; C490219:PLK4 protein, human; D017346:Protein Serine-Threonine Kinases
keywords: Centriole duplication; Centrosome; Cep78; Plk4