title: Effect of perfluorooctane sulfonate on viability, maturation and gap junctional intercellular communication of porcine oocytes in vitro.
abstract: Perfluorooctane sulfonate (PFOS) is a broadly used man-made surfactant whose long half-life has led to bioaccumulation. This perfluorinated compound is ubiquitous in human body fluids. PFOS concentrations as high as 26μM in plasma have been reported in occupationally exposed populations, and high levels of PFOS in human follicular fluid have been associated with subfertility. However, the effect of PFOS on the maturation of oocytes in mammals has not been reported to date. The aim of this study was to determine the effects of PFOS during oocyte maturation. Results indicate that PFOS inhibits oocyte viability (Lethal Concentration50=32μM) and maturation (inhibition of maturation50=22μM) at physiologically relevant concentrations. In order to evaluate the mechanisms of oocyte maturation inhibition by PFOS, gap junctional intercellular communication (GJIC) between oocytes and granulosa cells was assessed. GJIC between granulosa cells and the oocyte was significantly affected during the first 8h of maturation. However, the inhibitory effect of PFOS on GJIC was not due to an alteration on the expression of connexin genes Cx43, Cx45 and Cx60. These findings suggest that occupationally exposed populations could be at risk, and that PFOS might affect oocyte maturation by interfering the GJIC in the cumulus-oocyte complexes during the first hours of maturation.
authors.affiliation: Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco 186, 09340 CDMX, Mexico; Maestría en Biología de la Reproducción Animal, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco 186, 09340 CDMX, Mexico.
authors.forename: A
authors.initials: A
authors.lastname: Domínguez
authors.affiliation: Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco 186, 09340 CDMX, Mexico.
authors.forename: Z
authors.initials: Z
authors.lastname: Salazar
authors.affiliation: Departamento de Biología de la Reproducción, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco 186, 09340 CDMX, Mexico.
authors.forename: E
authors.initials: E
authors.lastname: Arenas
authors.affiliation: Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco 186, 09340 CDMX, Mexico.
authors.forename: M
authors.initials: M
authors.lastname: Betancourt
authors.affiliation: Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco 186, 09340 CDMX, Mexico.
authors.forename: Y
authors.initials: Y
authors.lastname: Ducolomb
authors.affiliation: Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco 186, 09340 CDMX, Mexico.
authors.forename: H
authors.initials: H
authors.lastname: González-Márquez
authors.affiliation: Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco 186, 09340 CDMX, Mexico.
authors.forename: E
authors.initials: E
authors.lastname: Casas
authors.affiliation: Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco 186, 09340 CDMX, Mexico; Maestría en Biología de la Reproducción Animal, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco 186, 09340 CDMX, Mexico.
authors.forename: M
authors.initials: M
authors.lastname: Teteltitla
authors.affiliation: Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco 186, 09340 CDMX, Mexico. Electronic address: mundo@xanum.uam.mx.
authors.forename: E
authors.initials: E
authors.lastname: Bonilla
mesh_terms: D017738:Alkanesulfonic Acids / Q000633:toxicity*; D000818:Animals; D002450:Cell Communication / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D017630:Connexins / Q000235:genetics; D005260:Female; D005466:Fluorocarbons / Q000633:toxicity*; D017629:Gap Junctions / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects; D006107:Granulosa Cells / Q000187:drug effects / Q000502:physiology; D009865:Oocytes / Q000187:drug effects* / Q000502:physiology; D013552:Swine
chemical_list: D017738:Alkanesulfonic Acids; D017630:Connexins; D005466:Fluorocarbons; C076994:perfluorooctane sulfonic acid
keywords: Connexin; Gap junction; Oocyte maturation; PFOS