title: [COMPARATIVE ANALYSIS OF TIGHT JUNCTIONS OF EPITHELIUM OF RATS JEJUNUM UNDER THE EFFECT OF LIPOPOLYSACCHARIDE AND CHOLERA TOXIN].
abstract: OBJECTIVE
  Comparative study of tight junctions and ultrastructure alterations of enterocytes of mucous membranes of jejunum of rats under the effect of lipopolysaccharides and cholera toxin.
  
  
  METHODS
  Lipopolysaccharides (Sigma-Aldrich, Germany) and cholera toxin (Sigma-Aldrich, Germany) were used. The study was carried out in Wistar line rats. Effect of lipopolysaccharides and cholera toxin on epitheliocytes was carried out by a method of withdrawal of segments of rat jejunum and their incubation with the specified substances. Comparative analysis of ultrathin sections of enterocytes of jejunum of rats and tight junctions between them was carried out in control and under the effect of lipopolysaccharides and cholera toxin.
  
  
  RESULTS
  Effect of lipopolysaccharides on ultrastructure of enterocytes of rat jejunum manifested in the change of cell form as a result of increase of intercellular space without destruction of tight junctions. Disappearance of desmosomes, increase of nuclei and more pronounced ER were noted in some epitheliocytes. Effect of cholerogen on epitheliocytes of mucous membrane of rat jejunum by a number of signs is similar to the effect of lipopolysaccharides, that manifested in an alteration of ultrastructure of cell, the form of those also transformed as a result of an increase of intercellular space, this process was not accompanied by destruction of tight junctions. Disappearance of folding of the lateral region of plasmatic membrane of cells and a reduction of a number of microvilli was observed under the effect of cholera toxin.
  
  
  CONCLUSIONS
  A similar character of effect of lipopolysaccharides and cholera toxins on ultrastructure of cells and region of tight junctions of enterocytes of rat jejunum was detected, both substances caused an increase of intercellular space without the destruction of tight junctions.
authors.forename: O N
authors.initials: ON
authors.lastname: Vishnevskaya
authors.forename: O V
authors.initials: OV
authors.lastname: Rybalchenko
authors.forename: I V
authors.initials: IV
authors.lastname: Larionov
authors.forename: O G
authors.initials: OG
authors.lastname: Orlova
authors.forename: A G
authors.initials: AG
authors.lastname: Markov
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000648:ultrastructure; D002772:Cholera Toxin / Q000494:pharmacology*; D003896:Desmosomes / Q000187:drug effects / Q000648:ultrastructure; D004847:Epithelial Cells / Q000166:cytology; D006801:Humans; D007583:Jejunum / Q000187:drug effects / Q000648:ultrastructure*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D051381:Rats; D019108:Tight Junctions / Q000187:drug effects / Q000648:ultrastructure*
chemical_list: D008070:Lipopolysaccharides; D002772:Cholera Toxin