title: Comparison of the properties of membranes produced with alginate and chitosan from mushroom and from shrimp.
abstract: Dense and porous chitosan-alginate membranes (1:1 in mass) useful as coverages of skin wounds treated through cell therapy were produced using chitosan of different chain sizes from fungal (white mushrooms) and animal (shrimp shells) sources. Porous materials were obtained by adding the surfactant Poloxamer 188 to the formulations. The influence of chitosan type on membranes physicochemical properties and toxicity to fibroblasts was evaluated. Porosity was noticed to be more pronounced in membranes obtained with fungal chitosan and increased with its molecular mass. These formulations showed the highest values of thickness, roughness, opacity, liquid uptake and water vapor permeability. The membranes were not toxic to fibroblasts, but the lowest cytotoxicity values (0.16-0.21%) were observed for membranes prepared with fungal chitosan in the presence of surfactant. In conclusion, it is possible to replace chitosan from animal sources by chitosan of fungal origin to produce membranes with negligible cytotoxicity while maintaining appropriate physicochemical properties.
authors.affiliation: Department of Engineering of Materials and of Bioprocesses, School of Chemical Engineering, University of Campinas, Av. Albert Einstein 500, CEP 13083-852 Campinas, SP, Brazil. Electronic address: andreakb@feq.unicamp.br.
authors.forename: Andréa C K
authors.initials: AC
authors.lastname: Bierhalz
authors.affiliation: Department of Engineering of Materials and of Bioprocesses, School of Chemical Engineering, University of Campinas, Av. Albert Einstein 500, CEP 13083-852 Campinas, SP, Brazil. Electronic address: ceciliabwestin@gmail.com.
authors.forename: Cecília Buzatto
authors.initials: CB
authors.lastname: Westin
authors.affiliation: Department of Engineering of Materials and of Bioprocesses, School of Chemical Engineering, University of Campinas, Av. Albert Einstein 500, CEP 13083-852 Campinas, SP, Brazil. Electronic address: ammoraes@feq.unicamp.br.
authors.forename: Ângela Maria
authors.initials: ÂM
authors.lastname: Moraes
mesh_terms: D000364:Agaricus / Q000737:chemistry*; D000464:Alginates / Q000737:chemistry / Q000302:isolation & purification; D000818:Animals; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry / Q000302:isolation & purification; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D020723:Glucuronic Acid / Q000737:chemistry / Q000302:isolation & purification; D006603:Hexuronic Acids / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D008422:Materials Testing; D008567:Membranes, Artificial; D033561:Penaeidae / Q000737:chemistry*
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D008567:Membranes, Artificial; D020723:Glucuronic Acid; D048271:Chitosan
keywords: Alginate; Cytotoxicity; Fungal chitosan; Membranes; Poloxamer 188; Wound dressings