title: Effects of inactivated porcine epidemic diarrhea virus on porcine monocyte-derived dendritic cells and intestinal dendritic cells.
abstract: Porcine epidemic diarrhea (PED) is a serious infection in neonatal piglets. As the causative agent of PED, porcine epidemic diarrhea virus (PEDV) results in acute diarrhea and dehydration with high mortality rates in swine. Dendritic cells (DCs) are highly effective antigen-presenting cells to uptake and present viral antigens to T cells, which then initiate a distinct immune response. In this study, our results show that the expression of Mo-DCs surface markers such as SWC3a(+)CD1a(+), SWC3a(+)CD80/86(+) and SWC3a(+)SLA-II-DR(+) is increased after incubation with UV-PEDV for 24h. Mo-DCs incubated with UV-PEDV produce higher levels of IL-12 and INF-γ compared to mock-infected Mo-DCs. Interactions between Mo-DCs and UV-PEDV significantly stimulate T-cell proliferation in vitro. Consistent with these results, there is an enhancement in the ability of porcine intestinal DCs to activate T-cell proliferation in vivo. We conclude that UV-PEDV may be a useful and safe vaccine to trigger adaptive immunity.
authors.affiliation: Nanjing Agricultural University, Wei gang 1, Jiangsu, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Gao
authors.affiliation: Nanjing Agricultural University, Wei gang 1, Jiangsu, PR China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Nanjing Agricultural University, Wei gang 1, Jiangsu, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Qin
authors.affiliation: Nanjing Agricultural University, Wei gang 1, Jiangsu, PR China.
authors.forename: Yinyan
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Nanjing Agricultural University, Wei gang 1, Jiangsu, PR China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Yu
authors.affiliation: Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, PR China. Electronic address: zxbyq@njau.edu.cn.
authors.forename: Qian
authors.initials: Q
authors.lastname: Yang
mesh_terms: D000818:Animals; D018352:Coronavirus Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D003713:Dendritic Cells / Q000276:immunology*; D003967:Diarrhea / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D007422:Intestines / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D009000:Monocytes / Q000276:immunology; D053485:Porcine epidemic diarrhea virus / Q000276:immunology*; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000821:virology; D013601:T-Lymphocytes / Q000276:immunology; D015164:Vaccines, Inactivated / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D015164:Vaccines, Inactivated; D014765:Viral Vaccines
keywords: Immunity; Inactivated porcine epidemic diarrhea virus; Intestinal DCs; Monocyte-derived dendritic cells (Mo-DCs); T cell proliferation