title: New metabolites of acteoside identified by ultra-performance liquid chromatography/quadrupole-time-of-flight MS(E) in rat plasma, urine, and feces.
abstract: Acteoside, which belongs to the family of phenylethanoid glycosides (PhGs), has extensive biological activities, including strong antioxidant, anti-inflammatory, hepatoprotect, and cell apoptosis regulation. Like other PhGs compounds, the fate of acteoside in the gut for both parent polyphenols and their degradation products, small phenolic acid and aromatic catabolites cannot be ignored. Therefore, in this work, expanded and systematical investigation for metabolism characteristic profiles of acteoside in vivo by ultra-performance liquid chromatography/ quadrupole-time-of-flight and a new MS(E) data collection technology had been studied. This was equivalent to non-slective MS/MS scans and helpful to explore new metabolites. After oral administration of 200mg/kg acteoside, He et al. (2011) a total of 44 metabolites was detected and identified, and 37 of them were reported for the first time. Among them, 35 were parent drug metabolites classified in 14 groups. Owen et al. (2003) Through the comprehensive metabolites study in plasma, urine and feces, acteoside systemical metabolites profiles and characteristics elaborated firstly. The relative content of metabolites research showed that acteoside could exist stably and the process for biotransformation of acteoside in blood keep extreme short time. Pan and Hori (1996) The significant new transformation of isomerization from acteoside to isoacteoside had been firstly found and confirmed. The results of this work provided new information for the clarification of the metabolism of acteoside and rendered a very valuable theoretical basis for the development of novel ideal dosage forms of acteoside in the future.
authors.affiliation: Jiangxi University of Traditional Chinese Medicine, No. 818 Yunwan Road, Nanchang 330002, PR China. Electronic address: sud94@aliyun.com.
authors.forename: Dan
authors.initials: D
authors.lastname: Su
authors.affiliation: Jiangxi University of Traditional Chinese Medicine, No. 818 Yunwan Road, Nanchang 330002, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: School of Pharmaceutical Science, Soochow University, Suzhou 215123, PR China.
authors.forename: Qiongming
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Jiangxi University of Traditional Chinese Medicine, No. 818 Yunwan Road, Nanchang 330002, PR China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Jiangxi University of Traditional Chinese Medicine, No. 818 Yunwan Road, Nanchang 330002, PR China; College of Chemistry and Chemical Engineering, Jiangxi Normal University, 99 Ziyang Road, Nanchang 330022, PR China.
authors.forename: Yonggui
authors.initials: Y
authors.lastname: Song
authors.affiliation: Jiangxi University of Traditional Chinese Medicine, No. 818 Yunwan Road, Nanchang 330002, PR China. Electronic address: fengyulin2003@hotmail.com.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Feng
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D005243:Feces / Q000737:chemistry; D005960:Glucosides / Q000032:analysis / Q000097:blood / Q000378:metabolism* / Q000652:urine; D007536:Isomerism; D008297:Male; D053858:Metabolic Networks and Pathways; D015394:Molecular Structure; D010636:Phenols / Q000032:analysis / Q000097:blood / Q000378:metabolism* / Q000652:urine; D051381:Rats; D017208:Rats, Wistar; D053719:Tandem Mass Spectrometry; D059808:Polyphenols
chemical_list: D005960:Glucosides; D010636:Phenols; C058956:acteoside; D059808:Polyphenols; C064683:isoacteoside
keywords: Acteoside; C-Heteroatom; Isomerization; Mass defect filter; Metabolites; Quadrupole-time of flight mass spectrometry