title: Analysis of CHIKV in Mosquitoes Infected via Artificial Blood Meal.
abstract: Having a mechanism to assess the transmission dynamics of a vector-borne virus is one critical component of understanding the life cycle of these viruses. Laboratory infection systems using artificial blood meals is one valuable approach for monitoring the progress of virus in its mosquito host and evaluating potential points for interruption of the cycle for control purposes. Here, we describe an artificial blood meal system with Chikungunya virus (CHIKV) and the processing of mosquito tissues and saliva to understand the movement and time course of virus infection in the invertebrate host.
authors.affiliation: Centers for Disease Control and Prevention, 3156 Rampart Road, Fort Collins, CO, 80521, USA.
authors.forename: Jeremy P
authors.initials: JP
authors.lastname: Ledermann
authors.affiliation: Centers for Disease Control and Prevention, 3156 Rampart Road, Fort Collins, CO, 80521, USA. apowers@cdc.gov.
authors.forename: Ann M
authors.initials: AM
authors.lastname: Powers
mesh_terms: D000330:Aedes / Q000821:virology*; D000821:Animal Feed / Q000821:virology*; D000818:Animals; D001802:Blood Substitutes; D002478:Cells, Cultured; D002646:Chikungunya virus / Q000472:pathogenicity*; D002522:Chlorocebus aethiops; D007303:Insect Vectors / Q000821:virology; D012463:Saliva / Q000821:virology; D014709:Vero Cells
chemical_list: D001802:Blood Substitutes
keywords: Artificial blood meal; Chikungunya virus; Mosquito infection; Mosquito processing; Saliva collection; Viral transmission dynamics