title: Selection of Recombinant Human Antibodies.
abstract: Since the development of therapeutic antibodies the demand of recombinant human antibodies is steadily increasing. Traditionally, therapeutic antibodies were generated by immunization of rat or mice, the generation of hybridoma clones, cloning of the antibody genes and subsequent humanization and engineering of the lead candidates. In the last few years, techniques were developed that use transgenic animals with a human antibody gene repertoire. Here, modern recombinant DNA technologies can be combined with well established immunization and hybridoma technologies to generate already affinity maturated human antibodies. An alternative are in vitro technologies which enabled the generation of fully human antibodies from antibody gene libraries that even exceed the human antibody repertoire. Specific antibodies can be isolated from these libraries in a very short time and therefore reduce the development time of an antibody drug at a very early stage.In this review, we describe different technologies that are currently used for the in vitro and in vivo generation of human antibodies.
authors.affiliation: Abteilung Biotechnologie, Institut für Biochemie, Biotechnologie und Bioinformatik, Technische Universität Braunschweig, Spielmannstr. 7, 38106, Braunschweig, Germany.|YUMAB GmbH, Rebenring 33, 38106, Braunschweig, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Tomszak
authors.affiliation: Abteilung Biotechnologie, Institut für Biochemie, Biotechnologie und Bioinformatik, Technische Universität Braunschweig, Spielmannstr. 7, 38106, Braunschweig, Germany.
authors.forename: Susanne
authors.initials: S
authors.lastname: Weber
authors.affiliation: Abteilung Biotechnologie, Institut für Biochemie, Biotechnologie und Bioinformatik, Technische Universität Braunschweig, Spielmannstr. 7, 38106, Braunschweig, Germany.
authors.forename: Jonas
authors.initials: J
authors.lastname: Zantow
authors.affiliation: Abteilung Biotechnologie, Institut für Biochemie, Biotechnologie und Bioinformatik, Technische Universität Braunschweig, Spielmannstr. 7, 38106, Braunschweig, Germany.|YUMAB GmbH, Rebenring 33, 38106, Braunschweig, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Schirrmann
authors.affiliation: Abteilung Biotechnologie, Institut für Biochemie, Biotechnologie und Bioinformatik, Technische Universität Braunschweig, Spielmannstr. 7, 38106, Braunschweig, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Hust
authors.affiliation: Abteilung Biotechnologie, Institut für Biochemie, Biotechnologie und Bioinformatik, Technische Universität Braunschweig, Spielmannstr. 7, 38106, Braunschweig, Germany. andre.frenzel@tu-bs.de.|YUMAB GmbH, Rebenring 33, 38106, Braunschweig, Germany. andre.frenzel@tu-bs.de.
authors.forename: André
authors.initials: A
authors.lastname: Frenzel
mesh_terms: D000818:Animals; D061067:Antibodies, Monoclonal, Humanized / Q000096:biosynthesis* / Q000302:isolation & purification*; D015723:Gene Library; D006801:Humans; D007114:Immunization; D051379:Mice; D019151:Peptide Library; D051381:Rats; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
chemical_list: D061067:Antibodies, Monoclonal, Humanized; D019151:Peptide Library; D011994:Recombinant Proteins
keywords: Human antibodies; Phage display; Recombinant antibodies; Ribosomal display; Transgenic animals; Yeast display