title: Differentiation of Rat Bone Marrow Mesenchymal Stem Cells Into Neuron-Like Cells In Vitro and Co-Cultured with Biological Scaffold as Transplantation Carrier.
abstract: BACKGROUND Autograft and allograft transplantation are used to prompt the regeneration of axons after nerve injury. However, the poor self-regeneration caused by the glial scar and growth inhibitory factors after neuronal necrosis limit the efficacy of these methods. The purpose of this study was to develop a new chitosan porous scaffold for cell seeding. MATERIAL AND METHODS The bone marrow mesenchymal stem cells (BMSCs) and tissue-engineered biomaterial scaffold compound were constructed and co-cultured in vitro with the differentiated BMSCs of Wistar rats and chitosan scaffold in a 3D environment. The purity of the third-generation BMSCs culture was identified using flow cytometry and assessment of induced neuronal differentiation. The scaffolds were prepared by the freeze-drying method. The internal structure of scaffolds and the change of cells' growth and morphology were observed under a scanning electron microscope. The proliferation of cells was detected with the MTT method. RESULTS On day 5 there was a significant difference in the absorbance value of the experimental group (0.549±0.0256) and the control group (0.487±0.0357) (P>0.05); but on day 7 there was no significant difference in the proliferation of the experimental group (0.751±0.011) and the control group and (0.78±0.017) (P>0.05). CONCLUSIONS Tissue engineering technology can provide a carrier for cells seeding and is expected to become an effective method for the regeneration and repair of nerve cells. Our study showed that chitosan porous scaffolds can be used for such purposes.
authors.affiliation: Department of Neurology, Tianjin Huanhu Hospital, Tianjing, China (mainland).
authors.forename: Wei
authors.initials: W
authors.lastname: Yue
authors.affiliation: Department of Neurosurgery, The Third Affiliate Hospital of Xi'an Jiaotong University, Shanxi Provincial People's Hospital, Xi'an, Shaanxi, China (mainland).
authors.forename: Feng
authors.initials: F
authors.lastname: Yan
authors.affiliation: Department of Neurosurgery, The Third Affiliate Hospital of Xi'an Jiaotong University, Shanxi Provincial People's Hospital, Xi'an, Shaanxi, China (mainland).
authors.forename: Yue-Lin
authors.initials: YL
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Tianjin Huanhu Hospital, Tianjing, China (mainland).
authors.forename: Shu-Ling
authors.initials: SL
authors.lastname: Liu
authors.affiliation: Department of Dermatovenereology, Tianjin Medical University General Hospital, Tianjing, China (mainland).
authors.forename: Shu-Ping
authors.initials: SP
authors.lastname: Hou
authors.affiliation: Department of Neurosurgery, The Third Affiliate Hospital of Xi'an Jiaotong University, Shanxi Provincial People's Hospital, Xi'an, Shaanxi, China (mainland).
authors.forename: Guo-Chao
authors.initials: GC
authors.lastname: Mao
authors.affiliation: School of Life Science and Technology, Xi'an Jiaotong University, Xi'an, Shaanxi, China (mainland).
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
authors.affiliation: Department of Neurology, Tianjin Huanhu Hospital, Tianjing, China (mainland).
authors.forename: Yong
authors.initials: Y
authors.lastname: Ji
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D048271:Chitosan; D018920:Coculture Techniques; D006412:Hematopoietic Stem Cells / Q000166:cytology; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009474:Neurons / Q000166:cytology*; D051381:Rats; D017208:Rats, Wistar; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D048271:Chitosan