title: Efficient Preparation of Streptochlorin from Marine Streptomyces sp. SYYLWHS-1-4 by Combination of Response Surface Methodology and High-Speed Counter-Current Chromatography.
abstract: Since first isolated from the lipophilic extract of Streptomyces sp. SF2583, streptochlorin, has attracted a lot of attention because of its various pharmacological properties, such as antibiotic, antiallergic, antitumor, and anti-inflammatory activities. For the efficient preparation of streptochlorin from a producing strain Streptomyces sp. SYYLWHS-1-4, we developed a combinative method by using response surface methodology (RSM) and high-speed counter-current chromatography (HSCCC). In the fermentation process, we used RSM to optimize the condition for the efficient accumulation of streptochlorin, and the optimal parameters were: yeast extract 1.889 g/L, soluble starch 8.636 g/L, K₂HPO₄ 0.359 g/L, CaCl₂ 2.5 g/L, MgSO₄ 0.625 g/L, marine salt 25 g/L, medium volume 50%, initial pH value 7.0, temperature 27.5 °C, which enhanced streptochlorin yield by 17.7-fold. During the purification process, the preparative HSCCC separation was performed using a petroleum ether-ethyl acetate-methanol-water (9:0.8:5:5, v/v/v/v) biphasic solvent system, where 300 mg of crude sample yielded 16.5 mg streptochlorin with over 95% purity as determined by UPLC. Consequently, the combination method provided a feasible strategy for highly effective preparation of streptochlorin, which ensured the supply of large amounts of streptochlorin for in vivo pharmacological assessments or other requirements.
authors.affiliation: LiDakSum Marine Biopharmaceutical Research Center, Ningbo University, Ningbo 315211, China. lilin0574@163.com.
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.affiliation: LiDakSum Marine Biopharmaceutical Research Center, Ningbo University, Ningbo 315211, China. heshan@nbu.edu.cn.
authors.forename: Shan
authors.initials: S
authors.lastname: He
authors.affiliation: LiDakSum Marine Biopharmaceutical Research Center, Ningbo University, Ningbo 315211, China. huahua20062008@126.com.
authors.forename: Lijian
authors.initials: L
authors.lastname: Ding
authors.affiliation: LiDakSum Marine Biopharmaceutical Research Center, Ningbo University, Ningbo 315211, China. 23yuanye@163.com.
authors.forename: Ye
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo 315211, China. zhupeng@nbu.edu.cn.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhu
authors.affiliation: Department of Biology, Northeastern University, Boston, MA 02115, USA. s.epstein@neu.edu.
authors.forename: Slava
authors.initials: S
authors.lastname: Epstein
authors.affiliation: Ningbo Boao Bioengineering Corporation, Ningbo 315201, China. fan@zjboao.cn.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Fan
authors.affiliation: Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo 315211, China. wuxiaokai@nbu.edu.cn.
authors.forename: Xiaokai
authors.initials: X
authors.lastname: Wu
authors.affiliation: Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo 315211, China. yanxiaojun@nbu.edu.cn.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Yan
mesh_terms: D001688:Biological Products / Q000737:chemistry / Q000302:isolation & purification; D002845:Chromatography / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D007211:Indoles / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D015394:Molecular Structure; D010080:Oxazoles / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D015203:Reproducibility of Results; D012997:Solvents; D013302:Streptomyces / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001688:Biological Products; D007211:Indoles; D010080:Oxazoles; D012997:Solvents; C526070:streptochlorin
keywords: Streptomyces sp. SYYLWHS-1-4; high-speed counter-current chromatography; preparation; response surface methodology; streptochlorin