title: Isolation of Pig Bone Marrow-Derived Mesenchymal Stem Cells.
abstract: Large animal models are an important preclinical tool for the evaluation of new interventions and their translation into clinical practice. The pig is a widely used animal model in multiple clinical fields, such as cardiology and orthopedics, and has been at the forefront of testing new therapeutics, including cell-based therapies. In the clinic, mesenchymal stem cells (MSCs) are used autologously, therefore isolated, and administrated into the same patient. For successful clinical translation of autologous approaches, the porcine model needs to test MSC in a similar manner. Since a limited number of MSCs can be isolated directly from the bone marrow, culturing techniques are needed to expand the population in vitro prior to therapeutic application. Here, we describe a protocol specifically tailored for the isolation and propagation of porcine-derived bone marrow MSCs.
authors.affiliation: Division Heart and Lungs, Department of Cardiology, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Dries A M
authors.initials: DA
authors.lastname: Feyen
authors.affiliation: Division Heart and Lungs, Department of Cardiology, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Frederieke
authors.initials: F
authors.lastname: van den Akker
authors.affiliation: Division Heart and Lungs, Department of Cardiology, University Medical Center Utrecht, Utrecht, The Netherlands.|Department of Cell Biology, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Willy
authors.initials: W
authors.lastname: Noort
authors.affiliation: Division Heart and Lungs, Department of Cardiology, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Steven A J
authors.initials: SA
authors.lastname: Chamuleau
authors.affiliation: Division Heart and Lungs, Department of Cardiology, University Medical Center Utrecht, Utrecht, The Netherlands.|Interuniversity Cardiology Institute of the Netherlands (ICIN), Utrecht, The Netherlands.
authors.forename: Pieter A
authors.initials: PA
authors.lastname: Doevendans
authors.affiliation: Division Heart and Lungs, Department of Cardiology, University Medical Center Utrecht, Utrecht, The Netherlands. j.sluijter@umcutrecht.nl.|Interuniversity Cardiology Institute of the Netherlands (ICIN), Utrecht, The Netherlands. j.sluijter@umcutrecht.nl.|Experimental Cardiology Laboratory, Department of Cardiology, University Medical Center Utrecht, Heidelberglaan 100, Room G02.523, Utrecht, CX, 3584, The Netherlands. j.sluijter@umcutrecht.nl.
authors.forename: Joost P G
authors.initials: JP
authors.lastname: Sluijter
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D034421:Sus scrofa; D013552:Swine; D014182:Transplantation, Autologous
keywords: Bone marrow; Cell culture; Expansion; Isolation; MSC; Porcine