title: Effects of bioirrigation of non-biting midges (Diptera: Chironomidae) on lake sediment respiration.
abstract: Bioirrigation or the transport of fluids into the sediment matrix due to the activities of organisms such as bloodworms (larvae of Diptera, Chironomidae), has substantial impacts on sediment respiration in lakes. However, previous quantifications of bioirrigation impacts of Chironomidae have been limited by technical challenges such as the difficulty to separate faunal and bacterial respiration. This paper describes a novel method based on the bioreactive tracer resazurin for measuring respiration in-situ in non-sealed systems with constant oxygen supply. Applying this new method in microcosm experiments revealed that bioirrigation enhanced sediment respiration by up to 2.5 times. The new method is yielding lower oxygen consumption than previously reported, as it is only sensitive to aerobic heterotrophous respiration and not to other processes causing oxygen decrease. Hence it decouples the quantification of respiration of animals and inorganic oxygen consumption from microbe respiration in sediment.
authors.affiliation: Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Müggelseedamm 310, 12587 Berlin, Germany.|Humboldt University of Berlin, Faculty of Mathematics and Natural Sciences, Geography Department, Rudower Chaussee 16, 12489 Berlin, Germany.
authors.forename: Viktor
authors.initials: V
authors.lastname: Baranov
authors.affiliation: Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Müggelseedamm 310, 12587 Berlin, Germany.|Humboldt University of Berlin, Faculty of Mathematics and Natural Sciences, Geography Department, Rudower Chaussee 16, 12489 Berlin, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Lewandowski
authors.affiliation: School of Geography, Earth and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
authors.forename: Paul
authors.initials: P
authors.lastname: Romeijn
authors.affiliation: Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Müggelseedamm 310, 12587 Berlin, Germany.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Singer
authors.affiliation: School of Geography, Earth and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
authors.forename: Stefan
authors.initials: S
authors.lastname: Krause
mesh_terms: D000332:Aerobiosis; D000818:Animals; D002683:Chironomidae / Q000254:growth & development* / Q000378:metabolism*; D019015:Geologic Sediments; D060106:Lakes; D010078:Oxazines / Q000378:metabolism; D010084:Oxidation-Reduction; D013194:Staining and Labeling / Q000379:methods*; D014966:Xanthenes / Q000378:metabolism
chemical_list: D010078:Oxazines; D014966:Xanthenes; C005843:resazurin