title: Erythrocyte senescence and membrane transporters in young and old rats.
abstract: Alterations at the level of plasma membrane are reported to play an important role in cellular senescence. The present study was undertaken to correlate cellular senescence, membrane transport processes and organismal aging. To achieve this objective activities of membrane linked Na+/K+ATPase (NKA), Na+/H+exchanger (NHE) and correlation with membrane hydrxyperoxide level, sialic acid content and membrane protein oxidation was studied in density-gradient fractionated young and old erythrocytes from 4 and 24 month old Wistar rats. The results reveal that cellular aging within the tissue is associated with significant decrease in activities of NKA and NHE of senescent erythrocytes in comparison to younger cell population of same age group. The result shows that impaired ion homeostasis due to altered membrane transporters including functional and compositional changes may be one of the reasons responsible behind rat erythrocyte aging.
authors.affiliation: a Department of Biochemistry , University of Allahabad , Allahabad , India.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Singh
authors.affiliation: a Department of Biochemistry , University of Allahabad , Allahabad , India.
authors.forename: Kanti Bhooshan
authors.initials: KB
authors.lastname: Pandey
authors.affiliation: a Department of Biochemistry , University of Allahabad , Allahabad , India.
authors.forename: Syed Ibrahim
authors.initials: SI
authors.lastname: Rizvi
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism / Q000473:pathology*; D016922:Cellular Senescence / Q000502:physiology*; D004912:Erythrocytes / Q000378:metabolism / Q000473:pathology*; D015151:Immunoblotting; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D050050:Protein Carbonylation; D051381:Rats; D017208:Rats, Wistar; D017923:Sodium-Hydrogen Exchangers / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
chemical_list: D008054:Lipid Peroxides; D008565:Membrane Proteins; D017923:Sodium-Hydrogen Exchangers; D000254:Sodium-Potassium-Exchanging ATPase
keywords: Erythrocyte; ion homeostasis; membrane transporters; senescence