title: Local administration of platelet-derived growth factor B (PDGFB) improves follicular development and ovarian angiogenesis in a rat model of Polycystic Ovary Syndrome.
abstract: Alterations in ovarian angiogenesis are common features in Polycystic Ovary Syndrome (PCOS) patients; the most studied of these alterations is the increase in vascular endothelial growth factor (VEGF) production by ovarian cells. Platelet-derived growth factor B (PDGFB) and D (PDGFD) are decreased in follicular fluid of PCOS patients and in the ovaries of a rat model of PCOS. In the present study, we aimed to analyze the effects of local administration of PDGFB on ovarian angiogenesis, follicular development and ovulation in a DHEA-induced PCOS rat model. Ovarian PDGFB administration to PCOS rats partially restored follicular development, decreased the percentage of cysts, increased the percentage of corpora lutea, and decreased the production of anti-Müllerian hormone. In addition, PDGFB administration improved ovarian angiogenesis by reversing the increase in periendothelial cell area and restoring VEGF levels. Our results shed light into the mechanisms that lead to altered ovarian function in PCOS and provide new data for potential therapeutic strategies.
authors.affiliation: Instituto de Biología y Medicina Experimental (IByME-CONICET), Vuelta de Obligado 2490, Buenos Aires, Argentina. Electronic address: dipietromari@gmail.com.
authors.forename: Mariana
authors.initials: M
authors.lastname: Di Pietro
authors.affiliation: Instituto de Biología y Medicina Experimental (IByME-CONICET), Vuelta de Obligado 2490, Buenos Aires, Argentina. Electronic address: leopoldinascotti@gmail.com.
authors.forename: Leopoldina
authors.initials: L
authors.lastname: Scotti
authors.affiliation: Instituto de Biología y Medicina Experimental (IByME-CONICET), Vuelta de Obligado 2490, Buenos Aires, Argentina. Electronic address: irustag@gmail.com.
authors.forename: Griselda
authors.initials: G
authors.lastname: Irusta
authors.affiliation: Instituto de Biología y Medicina Experimental (IByME-CONICET), Vuelta de Obligado 2490, Buenos Aires, Argentina; Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina. Electronic address: martatesone.uba@gmail.com.
authors.forename: Marta
authors.initials: M
authors.lastname: Tesone
authors.affiliation: Instituto de Biología y Medicina Experimental (IByME-CONICET), Vuelta de Obligado 2490, Buenos Aires, Argentina. Electronic address: fparborell@gmail.com.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Parborell
authors.affiliation: Instituto de Biología y Medicina Experimental (IByME-CONICET), Vuelta de Obligado 2490, Buenos Aires, Argentina. Electronic address: dnabramovich@gmail.com.
authors.forename: Dalhia
authors.initials: D
authors.lastname: Abramovich
mesh_terms: D000818:Animals; D054304:Anti-Mullerian Hormone; D005260:Female; D015571:Follicular Fluid / Q000187:drug effects* / Q000378:metabolism; D009389:Neovascularization, Pathologic / Q000188:drug therapy* / Q000378:metabolism; D006080:Ovarian Follicle / Q000187:drug effects* / Q000378:metabolism; D016471:Ovarian Hyperstimulation Syndrome / Q000188:drug therapy / Q000378:metabolism; D010060:Ovulation / Q000187:drug effects; D011085:Polycystic Ovary Syndrome / Q000188:drug therapy* / Q000378:metabolism; D020574:Proto-Oncogene Proteins c-sis / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D020574:Proto-Oncogene Proteins c-sis; D042461:Vascular Endothelial Growth Factor A; D054304:Anti-Mullerian Hormone
keywords: Angiogenesis; Ovary; Platelet derived growth factor; Polycystic ovary syndrome