title: Bond Strength of a Bisphenol-A-Free Fissure Sealant With and Without Adhesive Layer under Conditions of Saliva Contamination.
abstract: Dental sealants are important for prevention of carious lesions, if they have good shear strength. The aim of this study was to evaluate the shear bond strength (SBS) of two sealants to saliva-contaminated and non-contaminated enamel with and without an intermediate adhesive layer underneath the sealant. Ninety flat enamel surfaces from human third molars were randomly assigned to 6 groups (n=15): F (control): Fluroshield(tm) sealant; EWB (control): Embrace(tm) WetBond(tm); SB/F: Single Bond adhesive system + F; SB/EWB, s-SB/F and s-SB/EWB. In the s-SB/F and s-SB/EWB groups, the acid-etched enamel was contaminated with 0.01 mL of fresh human saliva for 20 s. Sealant cylinders were bonded to enamel surface with and without an intermediate adhesive system layer. The shear tests were performed using a universal testing machine (0.5 mm/min). Data were analyzed statistically by Kruskal-Wallis and Mann-Whitney tests (α=0.05). F presented higher mean SBS than EWB in all experimental conditions. The lowest SBS mean was obtained for EWB on contaminated enamel (p<0.05). In conclusion, an adhesive system layer should be used prior to sealant placement, in both dry and saliva-contaminated enamel. F had the best performance in all experimental conditions. EWB sealant showed very low results, but an adhesive layer underneath the sealant increased its SBS even after salivary contamination.
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, USP - Universidade de São Paulo, Ribeirão Preto, SP, Brazil, Universidade de São Paulo, Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto SP , Brazil.
authors.forename: Késsia Suênia Fidelis de
authors.initials: KS
authors.lastname: Mesquita-Guimarães
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, USP - Universidade de São Paulo, Ribeirão Preto, SP, Brazil, Universidade de São Paulo, Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto SP , Brazil.
authors.forename: Iliana Ferraz
authors.initials: IF
authors.lastname: Sabbatini
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, USP - Universidade de São Paulo, Ribeirão Preto, SP, Brazil, Universidade de São Paulo, Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto SP , Brazil.
authors.forename: Cintia Guimarães de
authors.initials: CG
authors.lastname: Almeida
authors.affiliation: Department of Dentistry, UFVJM - Federal University of Valleys of Jequitinhonha and Mucuri, Diamantina, MG, Brazil, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Department of Dentistry, Federal University of Valleys of Jequitinhonha and Mucuri, Diamantina MG , Brazil.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Galo
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, USP - Universidade de São Paulo, Ribeirão Preto, SP, Brazil, Universidade de São Paulo, Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto SP , Brazil.
authors.forename: Paulo
authors.initials: P
authors.lastname: Nelson-Filho
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, USP - Universidade de São Paulo, Ribeirão Preto, SP, Brazil, Universidade de São Paulo, Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto SP , Brazil.
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Borsatto
mesh_terms: D001559:Benzhydryl Compounds; D001840:Dental Bonding; D006801:Humans; D010636:Phenols; D010895:Pit and Fissure Sealants; D012463:Saliva / Q000737:chemistry*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D010636:Phenols; D010895:Pit and Fissure Sealants; C006780:bisphenol A; D000098826:Bisphenol A Compounds