title: Screening and identification of five peptides from pinto bean with inhibitory activities against α-amylase using phage display technique.
abstract: The objective of this study was to screen and identify α-amylase inhibitor peptides from Pinto bean. Five Pinto bean bioactive peptides were successfully identified: PPHMLP (P1), PLPWGAGF (P3), PPHMGGP (P6), PLPLHMLP (P7) and LSSLEMGSLGALFVCM (P9). Based on ELISA results, their promising optical density values were 1.27; 3.71, 1.67, 3.20 and 1.03, respectively, which indicated the binding interaction between the peptide and α-amylase occurred. The highest inhibitory activity (66.72%) of the chemically synthesized peptide was shown in SyP9 followed by SyP1 (48.86%), SyP3 (31.17%), SyP7 (27.88%) and SyP6 (23.96%). The IC50 values were 1.97, 8.96, 14.63, 18.45 and 20.56mgml(-1), respectively. Structure activity relationship study revealed that α-amylase was inhibited due to its residues of Ala230, Asp229, Asp326, Tyr54, Met195, Leu194 and His233 were bound. On the other hand, the residues of PBBP (i.e. histidine, proline and methionine) were found to have the highest potency in the binding interaction.
authors.affiliation: Analytical Biochemistry Research Centre, Universiti Sains Malaysia, 11800 Penang, Malaysia.
authors.forename: Ying-Yuan
authors.initials: YY
authors.lastname: Ngoh
authors.affiliation: Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800 Penang, Malaysia.
authors.forename: Theam Soon
authors.initials: TS
authors.lastname: Lim
authors.affiliation: Analytical Biochemistry Research Centre, Universiti Sains Malaysia, 11800 Penang, Malaysia. Electronic address: mattgan81@yahoo.com.
authors.forename: Chee-Yuen
authors.initials: CY
authors.lastname: Gan
mesh_terms: D000595:Amino Acid Sequence; D061505:Cell Surface Display Techniques; D003001:Cloning, Molecular; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D009842:Oligopeptides / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology; D010455:Peptides / Q000737:chemistry* / Q000235:genetics / Q000494:pharmacology*; D027805:Phaseolus / Q000737:chemistry* / Q000235:genetics; D037121:Plant Lectins / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D000516:alpha-Amylases / Q000037:antagonists & inhibitors*
chemical_list: D004791:Enzyme Inhibitors; D009842:Oligopeptides; D010455:Peptides; D037121:Plant Lectins; D010940:Plant Proteins; C061510:alpha-amylase inhibitor, Phaseolus vulgaris; D000516:alpha-Amylases
keywords: Bioactive peptides; Phage display; Pinto bean; α-amylase inhibitor