title: Metals of Deep Ocean Water Increase the Anti-Adipogenesis Effect of Monascus-Fermented Product via Modulating the Monascin and Ankaflavin Production.
abstract: Deep ocean water (DOW) obtained from a depth of more than 200 m includes abundant nutrients and minerals. DOW was proven to positively increase monascin (MS) and ankaflavin (AK) production and the anti-adipogenesis effect of Monascus-fermented red mold dioscorea (RMD). However, the influences that the major metals in DOW have on Monascus secondary metabolite biosynthesis and anti-adipogenesis remain unknown. Therefore, the major metals in DOW were used as the culture water to produce RMD. The secondary metabolites production and anti-adipogenesis effect of RMD cultured with various individual metal waters were investigated. In the results, the addition of water with Mg, Ca, Zn, and Fe increased MS and AK production and inhibited mycotoxin citrinin (CT). However, the positive influence may be contributed to the regulation of pigment biosynthesis. Furthermore, in the results of cell testing, higher lipogenesis inhibition was seen in the treatments of various ethanol extracts of RMD cultured with water containing Mg, K, Zn, and Fe than in those of RMD cultured with ultra-pure water. In conclusion, various individual metals resulted in different effects on MS and AK productions as well as the anti-adipogenesis effect of RMD, but the specific metals contained in DOW may cause synergistic or comprehensive effects that increase the significantly positive influence.
authors.affiliation: Department of Life Science, National Taitung University, 369, Section 2, University Rd., Taitung 95092, Taiwan. luck6950@gmail.com.
authors.forename: Tzu-Ying
authors.initials: TY
authors.lastname: Lung
authors.affiliation: Department of Life Science, National Taitung University, 369, Section 2, University Rd., Taitung 95092, Taiwan. bigli10118@livemail.tw.
authors.forename: Li-Ya
authors.initials: LY
authors.lastname: Liao
authors.affiliation: Department of Nutrition and Health Science, Fooyin University, Kaohsiung 83102, Taiwan. FT054@fy.edu.tw.
authors.forename: Jyh-Jye
authors.initials: JJ
authors.lastname: Wang
authors.affiliation: Department of Life Science, National Taitung University, 369, Section 2, University Rd., Taitung 95092, Taiwan. blwei@nttu.edu.tw.
authors.forename: Bai-Luh
authors.initials: BL
authors.lastname: Wei
authors.affiliation: Water Resource Division, Stone and Resource Industry R&amp;D Center, Hualian 973, Taiwan. koh@srdc.org.tw.
authors.forename: Ping-Yi
authors.initials: PY
authors.lastname: Huang
authors.affiliation: Department of Life Science, National Taitung University, 369, Section 2, University Rd., Taitung 95092, Taiwan. cllee@nttu.edu.tw.
authors.forename: Chun-Lin
authors.initials: CL
authors.lastname: Lee
mesh_terms: D050156:Adipogenesis / Q000187:drug effects*; D002953:Citrinin / Q000009:adverse effects; D027742:Dioscorea / Q000378:metabolism; D005285:Fermentation; D005415:Flavins / Q000494:pharmacology*; D006575:Heterocyclic Compounds, 3-Ring / Q000494:pharmacology*; D050155:Lipogenesis / Q000187:drug effects; D008670:Metals / Q000494:pharmacology*; D032741:Monascus / Q000378:metabolism*; D009183:Mycotoxins / Q000009:adverse effects; D009792:Oceans and Seas; D012623:Seawater
chemical_list: D005415:Flavins; D006575:Heterocyclic Compounds, 3-Ring; D008670:Metals; D009183:Mycotoxins; C499916:ankaflavin; D002953:Citrinin; C517880:monascin
keywords: Monascus; ankaflavin; deep ocean water; monascin; obesity