title: A tightly inducible riboswitch system in Synechocystis sp. PCC 6803.
abstract: Cyanobacteria are photosynthetic microorganisms that serve as experimental model organisms for the study of photosynthesis, environmental stress responses, and the production of biofuels. Genetic tools for bioengineering have been developed as a result of such studies. However, there is still room for improvement for the tight control of experimental protein expression in these microorganisms. Here, we describe an expression system controlled by a theophylline-responsive riboswitch that we have constructed in the cyanobacterium Synechocystis sp. PCC 6803. We demonstrate that, in response to different theophylline concentrations, this riboswitch can tightly control green fluorescence protein expression in Synechocystis. Thus, this system is useful as a tool for genetic engineering and the synthetic biology of cyanobacteria.
authors.affiliation: Department of Bioscience, Tokyo University of Agriculture.
authors.forename: Ryudo
authors.initials: R
authors.lastname: Ohbayashi
authors.forename: Hideto
authors.initials: H
authors.lastname: Akai
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Yoshikawa
authors.forename: Wolfgang R
authors.initials: WR
authors.lastname: Hess
authors.forename: Satoru
authors.initials: S
authors.lastname: Watanabe
mesh_terms: D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005818:Genetic Engineering; D049452:Green Fluorescent Proteins / Q000235:genetics; D010788:Photosynthesis; D058928:Riboswitch; D046939:Synechocystis / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D058615:Synthetic Biology; D013806:Theophylline / Q000494:pharmacology
chemical_list: D058928:Riboswitch; D049452:Green Fluorescent Proteins; D013806:Theophylline