title: Fatty acid and metabolomic profiling approaches differentiate heterotrophic and mixotrophic culture conditions in a microalgal food supplement 'Euglena'.
abstract: Microalgae have been recognized as a good food source of natural biologically active ingredients. Among them, the green microalga Euglena is a very promising food and nutritional supplements, providing high value-added poly-unsaturated fatty acids, paramylon and proteins. Different culture conditions could affect the chemical composition and food quality of microalgal cells. However, little information is available for distinguishing the different cellular changes especially the active ingredients including poly-saturated fatty acids and other metabolites under different culture conditions, such as light and dark.
  
  
  
  In this study, together with fatty acid profiling, we applied a gas chromatography-mass spectrometry (GC-MS)-based metabolomics to differentiate hetrotrophic and mixotrophic culture conditions.
  
  
  
  This study suggests metabolomics can shed light on understanding metabolomic changes under different culture conditions and provides a theoretical basis for industrial applications of microalgae, as food with better high-quality active ingredients.
authors.affiliation: Shenzhen Key Laboratory of Marine Bioresource & Eco-environmental Science, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, College of Life Science, Shenzhen University, Shenzhen, 518060, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Zeng
authors.affiliation: Shenzhen Key Laboratory of Marine Bioresource & Eco-environmental Science, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, College of Life Science, Shenzhen University, Shenzhen, 518060, People's Republic of China.|Nanshan District key lab for biopolymers and safety evaluation, Shenzhen University, Shenzhen, 518060, People's Republic of China.
authors.forename: Wenlong
authors.initials: W
authors.lastname: Hao
authors.affiliation: Shenzhen Key Laboratory of Marine Bioresource & Eco-environmental Science, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, College of Life Science, Shenzhen University, Shenzhen, 518060, People's Republic of China.
authors.forename: Yongdong
authors.initials: Y
authors.lastname: Zou
authors.affiliation: Laboratory of Synthetic Microbiology, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People's Republic of China.
authors.forename: Mengliang
authors.initials: M
authors.lastname: Shi
authors.affiliation: Shenzhen Key Laboratory of Marine Bioresource & Eco-environmental Science, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, College of Life Science, Shenzhen University, Shenzhen, 518060, People's Republic of China.
authors.forename: Yongguang
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Shenzhen Key Laboratory of Marine Bioresource & Eco-environmental Science, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, College of Life Science, Shenzhen University, Shenzhen, 518060, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Xiao
authors.affiliation: Shenzhen Key Laboratory of Marine Bioresource & Eco-environmental Science, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, College of Life Science, Shenzhen University, Shenzhen, 518060, People's Republic of China.
authors.forename: Anping
authors.initials: A
authors.lastname: Lei
authors.affiliation: Shenzhen Key Laboratory of Marine Bioresource & Eco-environmental Science, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, College of Life Science, Shenzhen University, Shenzhen, 518060, People's Republic of China.
authors.forename: Zhangli
authors.initials: Z
authors.lastname: Hu
authors.affiliation: Laboratory of Synthetic Microbiology, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People's Republic of China.
authors.forename: Weiwen
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Food Science and Engineering, College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen, Guangdong, China. snowyzlj@163.com.
authors.forename: Liqing
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Shenzhen Key Laboratory of Marine Bioresource & Eco-environmental Science, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, College of Life Science, Shenzhen University, Shenzhen, 518060, People's Republic of China.|Nanshan District key lab for biopolymers and safety evaluation, Shenzhen University, Shenzhen, 518060, People's Republic of China.
authors.forename: Jiangxin
authors.initials: J
authors.lastname: Wang
mesh_terms: D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods; D003470:Culture Media / Q000378:metabolism; D019587:Dietary Supplements / Q000382:microbiology*; D005055:Euglena / Q000145:classification / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism*; D064688:Metabolic Flux Analysis / Q000379:methods; D055442:Metabolome / Q000502:physiology*; D058086:Microalgae / Q000145:classification / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D003470:Culture Media; D005227:Fatty Acids
keywords: Euglena; Fatty acid; Heterotrophic; Lipids; Metabolomics; Mixotrophic