title: Soil-borne wheat mosaic virus infectious clone and manipulation for gene-carrying capacity.
abstract: A full-length infectious cDNA clone of soil-borne wheat mosaic virus (SBWMV; genus Furovirus; family Virgaviridae) was developed for agrobacterium delivery. The cloned virus can be agroinfiltrated to Nicotiana benthamiana for subsequent infection of wheat (Triticum aestivum, L.). The utility of the virus as a vector for gene silencing and expression was assessed through sequence insertions in multiple sites of RNA2. Virus-induced photobleaching was observed in N. benthamiana but not in wheat, despite the stability of the inserts. The SBWMV infectious clone can be used for further studies to investigate the biology of SBWMV through mutagenesis.
authors.affiliation: Center for Applied Plant Sciences, Ohio State University, Ohio Agriculture Research and Development Center (OARDC), Wooster, OH, USA.|Department of Plant Pathology, Ohio State University, OARDC, Wooster, OH, USA.
authors.forename: Sridhar
authors.initials: S
authors.lastname: Jarugula
authors.affiliation: Center for Applied Plant Sciences, Ohio State University, Ohio Agriculture Research and Development Center (OARDC), Wooster, OH, USA.|Centre for Tropical Crops and Biocommodities, Queensland University of Technology, Brisbane, QLD, 4000, Australia.
authors.forename: Steven R
authors.initials: SR
authors.lastname: Charlesworth
authors.affiliation: Center for Applied Plant Sciences, Ohio State University, Ohio Agriculture Research and Development Center (OARDC), Wooster, OH, USA.|Department of Plant Pathology, Ohio State University, OARDC, Wooster, OH, USA.
authors.forename: Feng
authors.initials: F
authors.lastname: Qu
authors.affiliation: Center for Applied Plant Sciences, Ohio State University, Ohio Agriculture Research and Development Center (OARDC), Wooster, OH, USA. lucy.stewart@ars.usda.gov.|Department of Plant Pathology, Ohio State University, OARDC, Wooster, OH, USA. lucy.stewart@ars.usda.gov.|USDA/ARS Corn, Soybean and Wheat Quality Research Unit, OARDC, 1680 Madison Ave, 023 Selby Hall, Wooster, OH, 44691, USA. lucy.stewart@ars.usda.gov.
authors.forename: Lucy R
authors.initials: LR
authors.lastname: Stewart
mesh_terms: D020868:Gene Silencing; D018014:Gene Transfer Techniques / Q000295:instrumentation; D005822:Genetic Vectors / Q000235:genetics* / Q000378:metabolism; D012328:RNA Viruses / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D012988:Soil Microbiology; D014026:Nicotiana / Q000235:genetics* / Q000821:virology; D014908:Triticum / Q000235:genetics* / Q000821:virology
keywords: Furovirus; Gene silencing; Infectious clone; SBWMV