title: Improved Butanol-Methanol (BUME) Method by Replacing Acetic Acid for Lipid Extraction of Biological Samples.
abstract: Extraction of lipids from biological samples is a critical step in lipidomics, especially for shotgun lipidomics where lipid extracts are directly infused into a mass spectrometer. The butanol-methanol (BUME) extraction method was originally developed to extract lipids from plasma samples with 1 % acetic acid. Considering some lipids are sensitive to acidic environments, we modified this protocol by replacing acetic acid with lithium chloride solution and extended the modified extraction to tissue samples. Although no significant reduction of plasmalogen levels in the acidic BUME extracts of rat heart samples was found, the modified method was established to extract various tissue samples, including rat liver, heart, and plasma. Essentially identical profiles of the majority of lipid classes were obtained from the extracts of the modified BUME and traditional Bligh-Dyer methods. However, it was found that neither the original, nor the modified BUME method was suitable for 4-hydroxyalkenal species measurement in biological samples.
authors.affiliation: Center for Metabolic Origins of Disease, Sanford Burnham Prebys Medical Discovery Institute, Orlando, FL, 32827, USA.
authors.forename: Mutya
authors.initials: M
authors.lastname: Cruz
authors.affiliation: Center for Metabolic Origins of Disease, Sanford Burnham Prebys Medical Discovery Institute, Orlando, FL, 32827, USA.
authors.forename: Miao
authors.initials: M
authors.lastname: Wang
authors.affiliation: Center for Metabolic Origins of Disease, Sanford Burnham Prebys Medical Discovery Institute, Orlando, FL, 32827, USA.
authors.forename: Jessica
authors.initials: J
authors.lastname: Frisch-Daiello
authors.affiliation: Center for Metabolic Origins of Disease, Sanford Burnham Prebys Medical Discovery Institute, Orlando, FL, 32827, USA. xhan@sbpdiscovery.org.
authors.forename: Xianlin
authors.initials: X
authors.lastname: Han
mesh_terms: D019342:Acetic Acid / Q000737:chemistry; D000818:Animals; D000440:Butanols / Q000737:chemistry; D005260:Female; D008055:Lipids / Q000032:analysis* / Q000302:isolation & purification; D018021:Lithium Chloride / Q000737:chemistry*; D008099:Liver / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry; D009206:Myocardium / Q000737:chemistry*; D010955:Plasmalogens / Q000302:isolation & purification; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000440:Butanols; D008055:Lipids; D010955:Plasmalogens; D018021:Lithium Chloride; D019342:Acetic Acid; D000432:Methanol
keywords: BUME; Lipid extraction; Lipidomics; Mass spectrometry; Plasmalogens; Shotgun lipidomics