title: In vivo bactericidal efficacy of farnesol on Ti6Al4V implants.
abstract: OBJECTIVE
  To evaluate the in vivo anti-staphylococcal bactericidal activity of farnesol on Ti6Al4V surfaces.
  
  
  METHODS
  An experimental model of infection in biomaterials was developed by inoculation of Staphylococcus aureus ATCC 29213 into the canal of both femurs of 15 Wistar rats. A Ti6Al4V pin impregnated with 30mM of farnesol was inserted into study femur, and a Ti6Al4V control was inserted into the control femur. To evaluate the bactericidal efficacy, a comparison was made between the median of the colony forming units recovered after inoculation in the study group and the control group for different times of euthanasia and inoculum size.
  
  
  RESULTS
  The median expressed as Log10 CFU counts obtained with farnesol titanium pin was 4.26, and in control group, it was 4.86, which was statistically significant (P=.001) on applying the Student t test for related samples. The median reduction obtained in farnesol pins relative to the control was 74%.
  
  
  CONCLUSIONS
  Treatment with farnesol 30mM on Ti6Al4V pins appears to decrease the rate of colonisation by Staphylococcus aureus.
authors.affiliation: Servicio de Cirugía Ortopédica y Traumatología, Complejo Hospitalario Universitario de Badajoz, Badajoz, España; Departamento de Terapéutica Medico Quirúrgica, Facultad de Medicina, Universidad de Extremadura, España. Electronic address: jaconstan@gmail.com.
authors.forename: J A
authors.initials: JA
authors.lastname: Constantino
authors.affiliation: Departamento de Ciencias Biomédicas, Área Microbiología, Facultad de Medicina, Universidad de Extremadura, España; Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN) (CIBER-BBN), España.
authors.forename: M
authors.initials: M
authors.lastname: Delgado-Rastrollo
authors.affiliation: Departamento de Física Aplicada, Facultad de Ciencias, Universidad de Extremadura, España; Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN) (CIBER-BBN), España.
authors.forename: M A
authors.initials: MA
authors.lastname: Pacha-Olivenza
authors.affiliation: Departamento de Ciencias Biomédicas, Área Microbiología, Facultad de Medicina, Universidad de Extremadura, España; Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN) (CIBER-BBN), España.
authors.forename: C
authors.initials: C
authors.lastname: Pérez-Giraldo
authors.affiliation: Servicio de Cirugía Ortopédica y Traumatología, Complejo Hospitalario Universitario de Badajoz, Badajoz, España; Departamento de Terapéutica Medico Quirúrgica, Facultad de Medicina, Universidad de Extremadura, España.
authors.forename: M
authors.initials: M
authors.lastname: Quiles
authors.affiliation: Departamento de Física Aplicada, Facultad de Ciencias, Universidad de Extremadura, España; Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN) (CIBER-BBN), España.
authors.forename: M L
authors.initials: ML
authors.lastname: González-Martín
authors.affiliation: Departamento de Física Aplicada, Facultad de Ciencias, Universidad de Extremadura, España; Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN) (CIBER-BBN), España.
authors.forename: A M
authors.initials: AM
authors.lastname: Gallardo-Moreno
mesh_terms: D000497:Alloys; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000627:therapeutic use; D001858:Bone Nails / Q000009:adverse effects* / Q000382:microbiology; D015169:Colony Count, Microbial; D005204:Farnesol / Q000008:administration & dosage* / Q000627:therapeutic use; D005269:Femur / Q000382:microbiology / Q000601:surgery; D008297:Male; D016459:Prosthesis-Related Infections / Q000175:diagnosis / Q000517:prevention & control*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000209:etiology / Q000517:prevention & control*; D013211:Staphylococcus aureus / Q000302:isolation & purification*; D014025:Titanium
chemical_list: D000497:Alloys; D000900:Anti-Bacterial Agents; C031462:titanium alloy (TiAl6V4); D005204:Farnesol; D014025:Titanium
keywords: Bactericida; Bactericidal; Farnesol; In vivo; Infección; Infection; Ti6Al4V