title: Intragenic Locus in Human PIWIL2 Gene Shares Promoter and Enhancer Functions.
abstract: Recently, more evidence supporting common nature of promoters and enhancers has been accumulated. In this work, we present data on chromatin modifications and non-polyadenylated transcription characteristic for enhancers as well as results of in vitro luciferase reporter assays suggesting that PIWIL2 alternative promoter in exon 7 also functions as an enhancer for gene PHYHIP located 60Kb upstream. This finding of an intragenic enhancer serving as a promoter for a shorter protein isoform implies broader impact on understanding enhancer-promoter networks in regulation of gene expression.
authors.affiliation: Department of Genomics and Postgenomic Technologies, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Yulia V
authors.initials: YV
authors.lastname: Skvortsova
authors.affiliation: Department of Genomics and Postgenomic Technologies, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Sofia A
authors.initials: SA
authors.lastname: Kondratieva
authors.affiliation: Department of Genomics and Postgenomic Technologies, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Marina V
authors.initials: MV
authors.lastname: Zinovyeva
authors.affiliation: Department of Genomics and Postgenomic Technologies, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Lev G
authors.initials: LG
authors.lastname: Nikolaev
authors.affiliation: Department of Genomics and Postgenomic Technologies, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Tatyana L
authors.initials: TL
authors.lastname: Azhikina
authors.affiliation: Department of Genomics and Postgenomic Technologies, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Ildar V
authors.identifier: http://orcid.org/0000-0003-1719-043X
authors.initials: IV
authors.lastname: Gainetdinov
mesh_terms: D060565:Argonaute Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D002843:Chromatin / Q000378:metabolism; D003198:Computer Simulation; D004742:Enhancer Elements, Genetic; D005091:Exons; D006801:Humans; D011061:Poly A / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D060565:Argonaute Proteins; D002843:Chromatin; C486868:PIWIL2 protein, human; D012333:RNA, Messenger; D011061:Poly A