title: Protein, enzyme and carbohydrate quantification using smartphone through colorimetric digitization technique.
abstract: In this paper the utilization of smartphone as a detection platform for colorimetric quantification of biological macromolecules has been demonstrated. Using V-channel of HSV color space, the quantification of BSA protein, catalase enzyme and carbohydrate (using D-glucose) have been successfully investigated. A custom designed android application has been developed for estimating the total concentration of biological macromolecules. The results have been compared with that of a standard spectrophotometer which is generally used for colorimetric quantification in laboratory settings by measuring its absorbance at a specific wavelength. The results obtained with the designed sensor is found to be similar when compared with the spectrophotometer data. The designed sensor is low cost, robust and we envision that it could promote diverse fields of bio-analytical investigations. Schematic illustration of the smartphone sensing mechanism for colorimetric analysis of biomolecular samples.
authors.affiliation: Applied Photonics and Nanophotonics Laboratory, Department of Physics, Tezpur University, Napaam, Assam, 784028, India.
authors.forename: Sibasish
authors.initials: S
authors.lastname: Dutta
authors.affiliation: Department of Computer science, Guwahati University, Gopinath Bordoloi Nagar, Guwahati, Assam, 781014, India.
authors.forename: Gunjan Prasad
authors.initials: GP
authors.lastname: Saikia
authors.affiliation: Applied Biochemistry Laboratory, Department of Molecular Biology and Biotechnology, Tezpur University, Napaam, Assam, 784028, India.
authors.forename: Dhruva Jyoti
authors.initials: DJ
authors.lastname: Sarma
authors.affiliation: Applied Microbiology and Biotechnology Laboratory, Department of Molecular Biology and Biotechnology, Tezpur University, Napaam, Assam, 784028, India.
authors.forename: Kuldeep
authors.initials: K
authors.lastname: Gupta
authors.affiliation: Applied Photonics and Nanophotonics Laboratory, Department of Physics, Tezpur University, Napaam, Assam, 784028, India.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Das
authors.affiliation: Applied Photonics and Nanophotonics Laboratory, Department of Physics, Tezpur University, Napaam, Assam, 784028, India.
authors.forename: Pabitra
authors.initials: P
authors.lastname: Nath
mesh_terms: D002241:Carbohydrates / Q000032:analysis*; D003116:Color; D003124:Colorimetry / Q000379:methods*; D004798:Enzymes / Q000032:analysis*; D005947:Glucose; D011506:Proteins / Q000032:analysis*; D000068997:Smartphone
chemical_list: D002241:Carbohydrates; D004798:Enzymes; D011506:Proteins; D005947:Glucose
keywords: Biomolecules; HSV color space; colorimetry; smartphone