title: Ameliorative Effect of Quercetin on Neurochemical and Behavioral Deficits in Rotenone Rat Model of Parkinson's Disease: Modulating Autophagy (Quercetin on Experimental Parkinson's Disease).
abstract: Autophagy is necessary for neuronal homeostasis and its dysfunction has been implicated in Parkinson's disease (PD) as it can exacerbate endoplasmic reticulum (ER) stress and ER stress-induced apoptosis. Quercetin is a flavonoid known for its neuroprotective and antioxidant effects. The present study investigated the protective, autophagy-modulating effects of quercetin in the rotenone rat model of PD. Rotenone was intraperitoneally injected at dose of 2 ml/kg/day for 4 weeks. Simultaneous intraperitoneal injection of quercetin was given at a dose of 50 mg/kg/day also for 4 weeks. Neurobehavioral changes were studied. Oxidative/antioxidant status, C/EBP homologous protein (CHOP), Beclin-1, and dopamine levels were assessed. DNA fragmentation and histopathological changes were evaluated. This research work revealed that quercetin significantly attenuated rotenone-induced behavioral impairment, augmented autophagy, ameliorated ER stress- induced apoptosis with attenuated oxidative stress. From the current study, quercetin can act as an autophagy enhancer in PD rat model and modulates the microenvironment that leads to neuronal death.
authors.affiliation: Biochemistry Department, Faculty of Medicine, Tanta University, Egypt.
authors.forename: Hemat E
authors.initials: HE
authors.lastname: El-Horany
authors.affiliation: Biochemistry Department, Faculty of Medicine, Tanta University, Egypt.
authors.forename: Rania N Abd
authors.initials: RN
authors.lastname: El-Latif
authors.affiliation: Clinical Pharmacology Department, Faculty of Medicine, Menoufia University, Egypt.
authors.forename: Maha M
authors.initials: MM
authors.lastname: ElBatsh
authors.affiliation: Physiology Department, Faculty of Medicine, Tanta University, Egypt. maroo_drmaroo@yahoo.com.
authors.forename: Marwa N
authors.initials: MN
authors.lastname: Emam
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001343:Autophagy / Q000187:drug effects*; D000071186:Beclin-1 / Q000235:genetics / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D002375:Catalepsy / Q000139:chemically induced / Q000188:drug therapy* / Q000235:genetics / Q000503:physiopathology; D060825:Cognitive Dysfunction / Q000139:chemically induced / Q000188:drug therapy* / Q000235:genetics / Q000503:physiopathology; D004298:Dopamine / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects; D005786:Gene Expression Regulation; D007274:Injections, Intraperitoneal; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress; D020734:Parkinsonian Disorders / Q000139:chemically induced / Q000188:drug therapy* / Q000235:genetics / Q000503:physiopathology; D011794:Quercetin / Q000494:pharmacology*; D051381:Rats; D012402:Rotenone; D015398:Signal Transduction; D013880:Thioredoxin-Disulfide Reductase / Q000235:genetics / Q000378:metabolism; D051742:Transcription Factor CHOP / Q000235:genetics / Q000378:metabolism
chemical_list: D000975:Antioxidants; D000071186:Beclin-1; C512829:Becn1 protein, rat; C497251:Ddit3 protein, rat; D018696:Neuroprotective Agents; D012402:Rotenone; D051742:Transcription Factor CHOP; D011794:Quercetin; D013880:Thioredoxin-Disulfide Reductase; D004298:Dopamine
keywords: Autophagy; ER Stress; Parkinson's Disease; Quercetin; Rotenone