title: Hyphomonas pacifica sp. nov., isolated from deep sea of the Pacific Ocean.
abstract: Three Gram-negative, aerobic, non-spore-forming, oval- to pear-shaped bacterial strains (T16B2(T), T24B3, and C76AD) were isolated from petroleum-degrading microbial communities through an enrichment of sediments and seawater samples from the Pacific Ocean. Phylogenetic analysis showed strains T16B2(T), T24B3, and C76AD to form a robust clade together with Hyphomonas atlanticus 22II1-22F38(T) and Hyphomonas beringensis 25B14_1(T) (16S rRNA identity ≥99.6 %). Genomic average nucleotide identity and DNA-DNA hybridization estimate values between strain T16B2(T) and nine type strains of the genus Hyphomonas are in the range of 82.9-88.2 and 18.3-33.6 %, respectively. The major cellular fatty acids in strains T16B2(T), T24B3, and C76AD are C16:0, C17:0, C18:1 ω7c-methyl, and summed feature 8 (C18:1 ω6c/ω7c). The DNA G+C content of strain T16B2(T) is 58.5 %. The predominant respiratory quinone of strain T16B2(T) is Q-11. Polar lipids comprise three unidentified glycolipids, one unidentified phospholipid, and two polar lipids. Combined phenotypic and genotypic data show strains T16B2(T), T24B3, and C76AD to represent a novel species of the genus Hyphomonas, for which the name Hyphomonas pacifica sp. nov. is proposed, with type strain T16B2(T) (=LMG 27911(T) = MCCC 1A04387(T)).
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources, Xiamen, 361005, China.|Key Laboratory of Marine Genetic Resources, The Third Institute of State Oceanic Administration, Xiamen, 361005, China.|Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen, 361005, China.|South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Xiamen, 361005, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources, Xiamen, 361005, China.|Key Laboratory of Marine Genetic Resources, The Third Institute of State Oceanic Administration, Xiamen, 361005, China.|Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen, 361005, China.|South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Xiamen, 361005, China.
authors.forename: Chongping
authors.initials: C
authors.lastname: Li
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources, Xiamen, 361005, China.|Key Laboratory of Marine Genetic Resources, The Third Institute of State Oceanic Administration, Xiamen, 361005, China.|Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen, 361005, China.|South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Xiamen, 361005, China.
authors.forename: Qiliang
authors.initials: Q
authors.lastname: Lai
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources, Xiamen, 361005, China.|Key Laboratory of Marine Genetic Resources, The Third Institute of State Oceanic Administration, Xiamen, 361005, China.|Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen, 361005, China.|South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Xiamen, 361005, China.
authors.forename: Guizhen
authors.initials: G
authors.lastname: Li
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources, Xiamen, 361005, China.|Key Laboratory of Marine Genetic Resources, The Third Institute of State Oceanic Administration, Xiamen, 361005, China.|Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen, 361005, China.|South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Xiamen, 361005, China.
authors.forename: Fengqin
authors.initials: F
authors.lastname: Sun
authors.affiliation: State Key Laboratory Breeding Base of Marine Genetic Resources, Xiamen, 361005, China. shaozz@163.com.|Key Laboratory of Marine Genetic Resources, The Third Institute of State Oceanic Administration, Xiamen, 361005, China. shaozz@163.com.|Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen, 361005, China. shaozz@163.com.|South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Xiamen, 361005, China. shaozz@163.com.
authors.forename: Zongze
authors.initials: Z
authors.lastname: Shao
mesh_terms: D005227:Fatty Acids / Q000378:metabolism; D016680:Genome, Bacterial; D019015:Geologic Sediments / Q000382:microbiology; D006017:Glycolipids / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010140:Pacific Ocean; D010743:Phospholipids / Q000378:metabolism; D010802:Phylogeny; D011809:Quinones / Q000378:metabolism; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA
chemical_list: D005227:Fatty Acids; D006017:Glycolipids; D010743:Phospholipids; D011809:Quinones
keywords: Deep-sea; Hyphomonas pacifica sp. nov.; Pacific Ocean; Taxonomy