title: Porcine myofibrillar proteins as potential precursors of bioactive peptides - an in silico study.
abstract: Selected porcine myofibrillar proteins have been assessed as potential precursors of bioactive peptides based on in silico analysis. The potential of protein sequences for releasing peptides was evaluated by determining the profile of their potential biological activity and the frequency of occurrence of fragments with a given activity using the BIOPEP database. Digestive enzymes: pepsin, trypsin and chymotrypsin have been used for the in silico proteolysis with the use of the "Enzyme(s) action" tool in BIOPEP. After simulated gastrointestinal digestion the tested sequences of pig myofibrillar proteins are a potential source of a total of 399 peptides with activities such as enzyme inhibition, antioxidative, hypotensive, stimulating or regulating various body functions and antiamnestic activities. Within the intact proteins and after simulated gastrointestinal digestion, dipeptidyl peptidase IV inhibitory peptide sequences were the most frequently observed. The results indicate that pork myofibrillar proteins are a promising source of peptides with biological activity.
authors.affiliation: Department of Meat Technology and Food Quality, University of Life Sciences in Lublin, ul. Skromna 8, Lublin 20-704, Poland. joanna.stadnik@up.lublin.pl.
authors.forename: Paulina
authors.initials: P
authors.lastname: Kęska
authors.forename: Joanna
authors.initials: J
authors.lastname: Stadnik
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002918:Chymotrypsin / Q000494:pharmacology*; D054873:Dipeptidyl-Peptidase IV Inhibitors / Q000494:pharmacology; D041981:Gastrointestinal Tract / Q000187:drug effects / Q000378:metabolism; D009210:Myofibrils / Q000737:chemistry*; D010434:Pepsin A / Q000494:pharmacology*; D013552:Swine; D014357:Trypsin / Q000494:pharmacology*
chemical_list: D054873:Dipeptidyl-Peptidase IV Inhibitors; D002918:Chymotrypsin; D014357:Trypsin; D010434:Pepsin A