title: Interactions of Pri-miRNA-34b/c and TP53 Polymorphisms on the Risk of Osteoporosis.
abstract: BACKGROUND
  Osteoporosis (OP) is a common, multifactorial disorder. Here, we investigated the association between polymorphisms in pri-miR-34b/c (rs4938723) and TP53 (Arg72Pro) and the prevalence of OP.
  
  
  METHODS
  A total of 681 individuals were assessed in a case-control study, including 310 patients with OP and 371 controls. Variants in pri-miR-34b/c and TP53 were identified using a polymerase chain reaction-restriction fragment length polymorphism method.
  
  
  RESULTS
  The presence of the CC and CT/CC pri-miR-34b/c genotypes were associated with a significantly reduced risk of OP compared with the TT genotype (CC vs. TT: odds ratio [OR] = 0.32, 95% confidence intervals [CI] = 0.17-0.59; p < 0.001; CT/CC vs. TT: OR = 0.69, 95% CI = 0.51-0.93; p = 0.016). The CC genotype was also associated with a significantly reduced OP risk compared with the TT/CT genotypes (OR = 0.35, 95% CI = 0.19-0.64; p < 0.001). Furthermore, compared with the carriers of the Arg72Pro GG genotype, carriers of the CC genotype had a 2.21-fold increased OP risk (95% CI = 1.45-3.37; p < 0.001) and CG/CC genotypes carriers had a 1.96-fold increased OP risk (95% CI = 1.39-2.76; p < 0.001).
  
  
  CONCLUSIONS
  The present findings indicate that pri-miR-34b/c rs4938723 and TP53 Arg72Pro polymorphisms may contribute to the risk of OP.
authors.affiliation: 1 Department of Orthopedic Surgery, West China Hospital of Sichuan University , Chengdu, China .|2 Department of Orthopedic, National Clinical Key Specialty, Yunnan Hospital, Kunming Medical University , Kunming, China .
authors.forename: Fu
authors.initials: F
authors.lastname: Jia
authors.affiliation: 3 Central Laboratory, Yunnan University of Chinese Traditional Medicine , Kunming, China .
authors.forename: Ruifen
authors.initials: R
authors.lastname: Sun
authors.affiliation: 1 Department of Orthopedic Surgery, West China Hospital of Sichuan University , Chengdu, China .
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.affiliation: 1 Department of Orthopedic Surgery, West China Hospital of Sichuan University , Chengdu, China .
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
authors.affiliation: 1 Department of Orthopedic Surgery, West China Hospital of Sichuan University , Chengdu, China .
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
authors.affiliation: 1 Department of Orthopedic Surgery, West China Hospital of Sichuan University , Chengdu, China .
authors.forename: Weili
authors.initials: W
authors.lastname: Fu
mesh_terms: D000328:Adult; D000368:Aged; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D002681:China; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D010024:Osteoporosis / Q000097:blood / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D016159:Tumor Suppressor Protein p53 / Q000097:blood / Q000235:genetics*
chemical_list: C522425:MIRN34 microRNA, human; D035683:MicroRNAs; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53