title: Assessing Gene Expression of the Endocannabinoid System.
abstract: Real-time quantitative reverse transcription polymerase chain reaction (real-time qRT-PCR), a major development of PCR technology, is a powerful and sensitive gene analysis technique that revolutionized the field of measuring gene expression. Here, we describe in detail RNA extraction, reverse transcription (RT), and relative quantification of genes belonging to the endocannabinoid system in mouse, rat, or human samples.
authors.affiliation: Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Teramo, Italy.
authors.forename: Mariangela
authors.initials: M
authors.lastname: Pucci
authors.affiliation: Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Teramo, Italy. cdaddario@unite.it.|European Center for Brain Research (CERC)/Santa Lucia Foundation, Rome, Italy. cdaddario@unite.it.
authors.forename: Claudio
authors.initials: C
authors.lastname: D'Addario
mesh_terms: D063388:Endocannabinoids / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D063388:Endocannabinoids
keywords: Endocannabinoid system genes; Fluorescent signals; RNA extraction; Real-time quantitative polymerase chain reaction; Reverse transcription