title: [HPLC Fingerprint of QingGuangAn and Determination of the Main Components].
abstract: OBJECTIVE
  To establish an HPLC fingerprint of ethanol extract of QingGuangAn, and to determine the contents of paeoniflorin and calycosin-7-glucosid.
  
  
  METHODS
  HPLC analysis was performed on an Agilent 1260 Infinity LC system and carried out at 35 degrees C on a column of GRACE Alltima C18 (250 mm x 4.6 mm, 5 μm). A binary gradient elution system was composed of acetonitrile (phase A) and water solution (phase B). Detection was performed at the wavelength of 254 nm, the mobile flow rate was 0.8 mL/min. A matrix including 20 variations (characteristic peaks area) and 10 samples was constructed for similarity evaluation.
  
  
  RESULTS
  The results showed that the collected samples had a good similarity. A specificity fingerprint was produced and 20 characteristic peaks were designated. The content of paeoniflorin and calycosin-7-glucosid was 0.368 and 0.049 mg/g, respectively.
  
  
  CONCLUSIONS
  It is a reliable, available and quick method for quality control of QingGuangAn,which provides some reference for the comparison of different extracting methods of QingGuangAn and the differences of pharmacodynamic.
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Bing-bing
authors.initials: BB
authors.lastname: Shen
authors.forename: Juan
authors.initials: J
authors.lastname: Luo
authors.forename: Yang
authors.initials: Y
authors.lastname: Chen
authors.forename: Yu-pei
authors.initials: YP
authors.lastname: Yang
authors.forename: Sheng-huang
authors.initials: SH
authors.lastname: Chen
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005960:Glucosides / Q000032:analysis*; D007529:Isoflavones / Q000032:analysis*; D039821:Monoterpenes / Q000032:analysis*; D011786:Quality Control; D006027:Glycosides
chemical_list: D004365:Drugs, Chinese Herbal; D005960:Glucosides; D007529:Isoflavones; D039821:Monoterpenes; C121707:7,3'-dihydroxy-4'-methoxyisoflavone; C015423:peoniflorin; D006027:Glycosides