title: Gel-Free/Label-Free Proteomic Analysis of Endoplasmic Reticulum Proteins in Soybean Root Tips under Flooding and Drought Stresses.
abstract: Soybean is a widely cultivated crop; however, it is sensitive to flooding and drought stresses. The adverse environmental cues cause the endoplasmic reticulum (ER) stress due to accumulation of unfolded or misfolded proteins. To investigate the mechanisms in response to flooding and drought stresses, ER proteomics was performed in soybean root tips. The enzyme activity of NADH cytochrome c reductase was two-fold higher in the ER than other fractions, indicating that the ER was isolated with high purity. Protein abundance of ribosomal proteins was decreased under both stresses compared to control condition; however, the percentage of increased ribosomes was two-fold higher in flooding compared to drought. The ER proteins related to protein glycosylation and signaling were in response to both stresses. Compared to control condition, calnexin was decreased under both stresses; however, protein disulfide isomerase-like proteins and heat shock proteins were markedly decreased under flooding and drought conditions, respectively. Furthermore, fewer glycoproteins and higher levels of cytosolic calcium were identified under both stresses compared to control condition. These results suggest that reduced accumulation of glycoproteins in response to both stresses might be due to dysfunction of protein folding through calnexin/calreticulin cycle. Additionally, the increased cytosolic calcium levels induced by flooding and drought stresses might disturb the ER environment for proper protein folding in soybean root tips.
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan.|National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba , Tsukuba 305-8572, Japan.|National Institute of Crop Science, National Agriculture and Food Research Organization , Tsukuba 305-8518, Japan.
authors.forename: Setsuko
authors.initials: S
authors.lastname: Komatsu
mesh_terms: D002118:Calcium / Q000378:metabolism; D055864:Droughts; D004721:Endoplasmic Reticulum / Q000737:chemistry*; D055868:Floods; D006023:Glycoproteins / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry; D017510:Protein Folding; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D013025:Glycine max / Q000737:chemistry*; D013312:Stress, Physiological
chemical_list: D006023:Glycoproteins; D020543:Proteome; D002118:Calcium
keywords: drought; endoplasmic reticulum; flooding; proteomics; root tip; soybean