title: Alteration of the cytokine signature by various TLR ligands in different T cell populations in MOG37-50 and MOG35-55-induced EAE in C57BL/6 mice.
abstract: Interleukin 17 (IL-17), produced by T cells, plays an important role in Multiple Sclerosis (MS) and its animal model, Experimental Autoimmune Encephalomyelitis (EAE). In contrast to IL-17-producing CD4+ T cells, the contribution of IL-17-producing CD8+ T cells (Tc17) in CNS autoimmunity has been investigated less intensively. Here we investigate the role of TC17 in EAE. We compare different T cell populations and their cytokine pattern in the MOG35-55- and MOG37-50-induced EAE. We detected a similar cytokine phenotype for both EAE models in the autoimmune process assessed at different stages. Regarding the migratory activity, an involvement of IL-17 and IFN-γ in disease onset was suggested. Furthermore, we show that PAMPs have the ability to drive autoimmune process. To modify the cytokine pattern of different T cell populations, a combination of distinct factors is required (the activation of MyD88 or Syk, the genetic background, the presence of APCs and CD4+ T cells).
authors.affiliation: Department of Neurology, Heinrich Heine University, Düsseldorf, Germany. Electronic address: Corinna.Steckner@gmail.com.
authors.forename: Corinna
authors.initials: C
authors.lastname: Steckner
authors.affiliation: Department of Neurology, Heinrich Heine University, Düsseldorf, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Weber
authors.affiliation: Department of Neurology, Heinrich Heine University, Düsseldorf, Germany.
authors.forename: Anne K
authors.initials: AK
authors.lastname: Mausberg
authors.affiliation: Department of Neurology, Heinrich Heine University, Düsseldorf, Germany.
authors.forename: Maximilian
authors.initials: M
authors.lastname: Heininger
authors.affiliation: Department of Neurology, Heinrich Heine University, Düsseldorf, Germany.
authors.forename: Felicitas
authors.initials: F
authors.lastname: Opdenhövel
authors.affiliation: Department of Neurology, Heinrich Heine University, Düsseldorf, Germany.
authors.forename: Bernd C
authors.initials: BC
authors.lastname: Kieseier
authors.affiliation: Department of Neurology, Heinrich Heine University, Düsseldorf, Germany.
authors.forename: Hans P
authors.initials: HP
authors.lastname: Hartung
authors.affiliation: Department of Neurology, Heinrich Heine University, Düsseldorf, Germany.
authors.forename: Harald H
authors.initials: HH
authors.lastname: Hofstetter
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D002478:Cells, Cultured; D002490:Central Nervous System / Q000276:immunology / Q000378:metabolism; D016207:Cytokines / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000378:metabolism; D058501:Enzyme-Linked Immunospot Assay; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression / Q000187:drug effects / Q000276:immunology; D006151:Guanosine / Q000031:analogs & derivatives / Q000276:immunology / Q000494:pharmacology; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D020381:Interleukin-17 / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D008024:Ligands; D008070:Lipopolysaccharides / Q000276:immunology / Q000494:pharmacology; D008810:Mice, Inbred C57BL; D063308:Myelin-Oligodendrocyte Glycoprotein / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016176:T-Lymphocyte Subsets / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D013997:Time Factors; D051193:Toll-Like Receptors / Q000819:agonists / Q000276:immunology* / Q000378:metabolism
chemical_list: D016207:Cytokines; D020381:Interleukin-17; D008024:Ligands; D008070:Lipopolysaccharides; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments; D051193:Toll-Like Receptors; C445081:myelin oligodendrocyte glycoprotein (35-55); D006151:Guanosine; D007371:Interferon-gamma; C068562:loxoribine
keywords: EAE; IL-17; MOG; T cell; TLR