title: Transcription of a novel P450 gene varies with some factors (pollutant exposure, temperature, time, and body region) in a marine oligochaete (Thalassodrilides sp.).
abstract: Cytochrome P450 (CYP) enzymes play important roles in the metabolism of exogenous compounds such as polycyclic aromatic hydrocarbons (PAHs). A novel, full-length CYP gene (CYP4V30) was identified in the oligochaete Thalassodrilides sp. CYP4V30 mRNA expression was studied in worms exposed to PAH-polluted (Σ16PAHs; 37441ng/g dry weight) or unpolluted (Σ16PAHs; 19ng/g dry weight) sediment. CYP4V30 expression was much higher in worms exposed to contaminated sediments than in those exposed to unpolluted sediments at some temperatures (20 and 25°C) and exposure durations (11-fold increase at 20°C, 10-day exposure), but not at 15°C or other exposure durations (P<0.05). CYP4V30 mRNA expression was higher in the middle of the body than in the posterior (P<0.05). The variation in transcriptional response with exposure time, temperature, and body region indicates that these factors should be considered when monitoring marine sediment pollution.
authors.affiliation: National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency, Maruishi 2-17-5, Hatsukaichi, Hiroshima 739-0452, Japan. Electronic address: nozakim@affrc.go.jp.
authors.forename: Mana
authors.initials: M
authors.lastname: Ito
authors.affiliation: National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency, Maruishi 2-17-5, Hatsukaichi, Hiroshima 739-0452, Japan. Electronic address: katsuit@affrc.go.jp.
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Ito
authors.affiliation: South Ehime Fisheries Research Center, Ehime University, 1289-1, Funakoshi, Ainan, Ehime 798-4292, Japan. Electronic address: kohta@agr.ehime-u.ac.jp.
authors.forename: Kohei
authors.initials: K
authors.lastname: Ohta
authors.affiliation: National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency, Maruishi 2-17-5, Hatsukaichi, Hiroshima 739-0452, Japan. Electronic address: hntks@affrc.go.jp.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Hano
authors.affiliation: National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency, Maruishi 2-17-5, Hatsukaichi, Hiroshima 739-0452, Japan. Electronic address: onduka@affrc.go.jp.
authors.forename: Toshimitsu
authors.initials: T
authors.lastname: Onduka
authors.affiliation: National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency, Maruishi 2-17-5, Hatsukaichi, Hiroshima 739-0452, Japan. Electronic address: kmochida@affrc.go.jp.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Mochida
mesh_terms: D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism; D004784:Environmental Monitoring; D019015:Geologic Sediments; D009835:Oligochaeta / Q000502:physiology*; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity; D013312:Stress, Physiological; D013696:Temperature; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; D003577:Cytochrome P-450 Enzyme System
keywords: CYP4; Oligochaeta; Polycyclic aromatic hydrocarbon; Temperature response; Xenobiotics; mRNA expression