title: Orbitide Composition of the Flax Core Collection (FCC).
abstract: The flax (Linum usitatissimum L.) core collection (FCC) was regenerated in Saskatoon, Saskatchewan and Morden, Manitoba in 2009. Seed orbitide content and composition from successfully propagated plants of 391 accessions were analyzed using high-throughput analyses employing high-performance liquid chromatography (HPLC) with reverse-phase monolithic HPLC columns and diode array detection (HPLC-DAD). Seed from plants regenerated in Morden had comparatively higher orbitide content than those grown in Saskatoon. Concentrations of orbitides encoded by contig AFSQ01016651.1 (1, 3, and 8) were higher than those encoded by AFSQ01025165.1 (6, 13, and 17) for most accessions in both locations. The cultivar 'Primus' from Poland and an unnamed accession (CN 101580 of unknown origin) exhibited the highest ratio of sum of [1,3,8] to a sum of [6,13,17]. Conversely, the lowest orbitide concentrations and ratio of [1,3,8] to [6,13,17] were observed in cultivars 'Hollandia' and 'Z 11637', both from The Netherlands. Orbitide expression did not correlate with flax morphological and other chemical traits.
authors.forename: Peta-Gaye Gillian
authors.initials: PG
authors.lastname: Burnett
authors.forename: Clara Marisa
authors.initials: CM
authors.lastname: Olivia
authors.forename: Denis Paskal
authors.initials: DP
authors.lastname: Okinyo-Owiti
authors.affiliation: Guangdong Saskatchewan Oilseed Joint Laboratory, Department of Food Science and Engineering, Jinan University , Guangzhou, Guangdong 510632, China.
authors.forename: Martin John Tarsisius
authors.initials: MJ
authors.lastname: Reaney
mesh_terms: D001901:Botany / Q000458:organization & administration; D002170:Canada; D002851:Chromatography, High Pressure Liquid; D016208:Databases, Factual; D019597:Flax / Q000737:chemistry* / Q000145:classification; D013058:Mass Spectrometry; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry / Q000145:classification
chemical_list: D010936:Plant Extracts
keywords: Linum usitatissimum L.; chromatography; cyclolinopeptides; germplasm characterization; orbitides; plant genetic resources