title: LC-MS/MS determination and interaction of the main components from the traditional Chinese drug pair Danshen-Sanqi based on rat intestinal absorption.
abstract: The Chinese drug pair Danshen (Salvia miltiorrhiza)-Sanqi (Panax ginseng) has been widely used for centuries treating various cardiovascular disorders, among which salvianlic acid B (SAB), ginsenoside Rg1(GRg1), ginsenoside Rb1(GRb1) and notoginsenoside R1(NGR1) were identified as the major components. The present study focused on the interaction between these components based on investigating their intestinal absorption using the Ussing chamber technique. The concentrations of SAB, GRg1, GRb1 and NGR1in the intestinal perfusate were determined by LC-MS/MS method, followed by Q (accumulative quantity) and Papp(apparent permeability). The results showed that all these four main components displayed very low permeabilities, which implied their poor absorption in the rat intestine. The intestinal absorption level of SAB displayed regioselectivity: duodenum < jejunum < ileum. However, there was no significant difference in the absorption of GRg1and GRb1in the different segments. The Q and Pappvalues of the four main components were obviously increased in jejunum when co-administrating Danshen extract with Sanqi extract. In conclusion, compatibility of Danshen and Sanqi could remarkably improve the intestinal absorption level of the main components in the pair. To some extent, this might explain the nature of the compatibility mechanisms of composite formulae in TCMs.
authors.affiliation: Guangdong Provincial Hospital of Chinese Medicine, The 2nd Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Huang
authors.affiliation: Guangdong Provincial Hospital of Chinese Medicine, The 2nd Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Guangdong Provincial Hospital of Chinese Medicine, The 2nd Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, China.
authors.forename: Junqi
authors.initials: J
authors.lastname: Bai
authors.affiliation: Guangdong Provincial Hospital of Chinese Medicine, The 2nd Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Xu
authors.affiliation: Guangdong Provincial Hospital of Chinese Medicine, The 2nd Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, China.
authors.forename: Dinghong
authors.initials: D
authors.lastname: Wu
authors.affiliation: Guangdong Provincial Hospital of Chinese Medicine, The 2nd Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Qiu
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D007408:Intestinal Absorption; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; C587496:sanqi
keywords: Danshen; Sanqi; Ussing chamber; intestinal absorption interaction