title: Theaflavin Synthesized in a Selective, Domino-Type, One-Pot Enzymatic Biotransformation Method with Camellia sinensis Cell Culture Inhibits Weight Gain and Fat Accumulation to High-Fat Diet-Induced Obese Mice.
abstract: The polyphenolic compound theaflavin, which is the main red pigment present in black tea, is reported to elicit various physiological effects. Because of the extremely low concentration of theaflavin present in black tea, its extraction from black tea leaves in quantities sufficient for use in medical studies has been difficult. We have developed a simple, inexpensive, selective, domino-type, one-pot enzymatic biotransformation method for the synthesis of theaflavin that is suitable for use in medical studies. Subsequent administration of this synthetic theaflavin to high-fat diet-induced obese mice inhibited both body weight gain and visceral fat accumulation, with no significant difference in the amount of faeces between the experimental and control mice.
authors.affiliation: School of Pharmaceutical Sciences, Ohu University.
authors.forename: Masumi
authors.initials: M
authors.lastname: Takemoto
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Takemoto
authors.forename: Ryoyasu
authors.initials: R
authors.lastname: Saijo
mesh_terms: D000818:Animals; D044946:Biflavonoids / Q000096:biosynthesis / Q000737:chemistry / Q000627:therapeutic use; D001711:Biotransformation; D028241:Camellia sinensis / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D002392:Catechin / Q000096:biosynthesis / Q000737:chemistry / Q000627:therapeutic use; D059305:Diet, High-Fat; D050152:Intra-Abdominal Fat / Q000187:drug effects; D008297:Male; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000188:drug therapy*; D010544:Peroxidases / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D015430:Weight Gain / Q000187:drug effects
chemical_list: D044946:Biflavonoids; D010936:Plant Extracts; C056068:theaflavin; D002392:Catechin; D010544:Peroxidases