title: The study of the relation of DNA repair pathway genes SNPs and the sensitivity to radiotherapy and chemotherapy of NSCLC.
abstract: To analyze the relation between SNPs in DNA repair pathway-related genes and sensitivity of tumor radio-chemotherapy, 26 SNPs in 20 DNA repair genes were genotyped on 176 patients of NSCLC undertaking radio-chemotherapy treatment. In squamous cell carcinoma (SCC), as the rs2228000, rs2228001 (XPC), rs2273953 (TP73), rs2279744 (MDM2), rs2299939 (PTEN) and rs8178085, rs12334811 (DNA-PKcs) affected the sensitivity to chemotherapy, so did the rs8178085, rs12334811 to radiotherapy. Moreover rs344781, rs2273953 and rs12334811 were related with the survival time of SCC. In general, the "good" genotype GG (rs12334811) showed greater efficacy of radio-chemotherapy and MSF (24 months) on SCC. In adenocarcinoma, as the rs2699887 (PIK3), rs12334811 (DNA-PKcs) influenced the sensitivity to chemotherapy, so did the rs2299939, rs2735343 (PTEN) to radiotherapy. And rs402710, rs80270, rs2279744 and rs2909430 impacted the survival time of the adenocarcinoma patients. Both GG (rs2279744) and AG (rs2909430) showed a shorter survival time (MFS = 6). Additionally, some SNPs such as rs2228000, rs2228001 and rs344781 were found to regulate the expression of DNA repair pathway genes through eQTLs dataset analysis. These results indicate that SNPs in DNA repair pathway genes might regulate the expression and affect the DNA damage repair, and thereby impact the efficacy of radio-chemotherapy and the survival time of NSCLC.
authors.affiliation: School of Life Science and Technology, Harbin Institute of Technology, Heilongjiang, China.|Department of Radiotherapy, Affiliated Tumour Hospital of Harbin Medical University.
authors.forename: Chunbo
authors.initials: C
authors.lastname: Wang
authors.affiliation: School of Life Science and Technology, Harbin Institute of Technology, Heilongjiang, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Nie
authors.affiliation: School of Life Science and Technology, Harbin Institute of Technology, Heilongjiang, China.
authors.forename: Yiqun
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Life Science and Technology, Harbin Institute of Technology, Heilongjiang, China.|Genome Analysis Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, China.
authors.forename: Guiyou
authors.initials: G
authors.lastname: Liu
authors.affiliation: Department of Radiotherapy, Beijing Miyun County Hospital.
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Life Science and Technology, Harbin Institute of Technology, Heilongjiang, China.
authors.forename: Shijie
authors.initials: S
authors.lastname: Xing
authors.affiliation: Department of Radiotherapy, Affiliated Tumour Hospital of Harbin Medical University.
authors.forename: Liping
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Radiotherapy, Affiliated Tumour Hospital of Harbin Medical University.
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.affiliation: School of Life Science and Technology, Harbin Institute of Technology, Heilongjiang, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Chen
authors.affiliation: School of Life Science and Technology, Harbin Institute of Technology, Heilongjiang, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy* / Q000235:genetics / Q000532:radiotherapy*; D004260:DNA Repair; D051747:DNA-Activated Protein Kinase / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D005260:Female; D053263:Gene Regulatory Networks; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000532:radiotherapy*; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000235:genetics; D051059:PTEN Phosphohydrolase / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D051736:Proto-Oncogene Proteins c-mdm2 / Q000235:genetics; D016019:Survival Analysis; D016896:Treatment Outcome; D000072160:Tumor Protein p73 / Q000235:genetics; D055815:Young Adult
chemical_list: D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C000606460:TP73 protein, human; D000072160:Tumor Protein p73; C086984:XPC protein, human; C497206:MDM2 protein, human; D051736:Proto-Oncogene Proteins c-mdm2; D051747:DNA-Activated Protein Kinase; C497263:PRKDC protein, human; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human