title: Haemonchus contortus excretory and secretory proteins (HcESPs) suppress functions of goat PBMCs in vitro.
abstract: Excretory and secretory products (ESPs) of nematode contain various proteins which are capable of inducing the instigation or depression of the host immune response and are involved in the pathogenesis of the worms. In the present study, Haemonchus contortus excretory and secretory products (HcESPs) were collected from the adult worms. Binding of HcESPs to goat peripheral blood mononuclear cells (PBMCs) was confirmed by immune-fluorescence assay. Effects of the HcESPs on cytokine production, cell proliferation, cell migration and nitric oxide (NO) production of PBMCs were checked by co-incubation of HcESPs with goat PBMCs. The results indicated that the production of IL-4 and IFN-γ were significantly decreased by HcESPs in dose dependent manner. On the contrary, the production of IL-10 and IL-17 were increased. Cell migration was significantly enhanced by HcESPs, whereas, HcESPs treatment significantly suppressed the cell proliferation and NO production. These results indicated that the HcESPs played important suppressive regulatory roles on PBMCs and provided highlights to the understanding of the host-parasite interactions.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, PR China.
authors.forename: Javaid Ali
authors.initials: JA
authors.lastname: Gadahi
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, PR China.
authors.forename: Bu
authors.initials: B
authors.lastname: Yongqian
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, PR China.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Ehsan
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, PR China.
authors.forename: Zhen Chao
authors.initials: ZC
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, PR China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, PR China.
authors.forename: Ruo Feng
authors.initials: RF
authors.lastname: Yan
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, PR China.
authors.forename: Xiao Kai
authors.initials: XK
authors.lastname: Song
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, PR China.
authors.forename: Li Xin
authors.initials: LX
authors.lastname: Xu
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, PR China.
authors.forename: Xiang Rui
authors.initials: XR
authors.lastname: Li
mesh_terms: D000818:Animals; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D006041:Goats; D006189:Haemonchus / Q000276:immunology* / Q000378:metabolism; D015801:Helminth Proteins / Q000276:immunology* / Q000378:metabolism; D006790:Host-Parasite Interactions / Q000276:immunology*; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D020381:Interleukin-17 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000378:metabolism; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism
chemical_list: D015801:Helminth Proteins; D020381:Interleukin-17; D016753:Interleukin-10; D015847:Interleukin-4; D009569:Nitric Oxide; D007371:Interferon-gamma
keywords: ESP; Haemonchus contortus; Immune response; Immunity; Immunology and Microbiology Section; PBMC; goat; immunomodulation