title: The SCAR/WAVE complex polarizes PAN receptors and promotes division asymmetry in maize.
abstract: Pre-mitotic establishment of polarity is a key event in the preparation of mother cells for asymmetric cell divisions that produce daughters of distinct fates, and ensures correct cellular patterning of tissues and eventual organ function. Previous work has shown that two receptor-like kinases, PANGLOSS2 (PAN2) and PAN1, and the small GTPase RHO GTPASE OF PLANTS (ROP) promote mother cell polarity and subsequent division asymmetry in developing maize stomata. PAN proteins become polarized prior to asymmetric cell division, however, the mechanism of this polarization is unknown. Here we show that the SCAR/WAVE regulatory complex, which activates the actin-nucleating ARP2/3 complex, is the first known marker of polarity in this asymmetric division model and is required for PAN polarization. These findings implicate actin, and specifically branched actin networks, in PAN polarization and asymmetric cell division.
authors.affiliation: Section of Cell and Developmental Biology, University of California San Diego, La Jolla, California 92093-0116, USA.
authors.forename: Michelle R
authors.identifier: http://orcid.org/0000-0002-6214-9359
authors.initials: MR
authors.lastname: Facette
authors.affiliation: Section of Cell and Developmental Biology, University of California San Diego, La Jolla, California 92093-0116, USA.
authors.forename: Yeri
authors.initials: Y
authors.lastname: Park
authors.affiliation: Section of Cell and Developmental Biology, University of California San Diego, La Jolla, California 92093-0116, USA.
authors.forename: Dena
authors.initials: D
authors.lastname: Sutimantanapi
authors.affiliation: Department of Molecular Biology, University of Wyoming, Laramie, Wyoming 82071, USA.
authors.forename: Anding
authors.initials: A
authors.lastname: Luo
authors.affiliation: Department of Plant Biology, Carnegie Institution of Science, 260 Panama Street, Stanford, California 94305, USA.
authors.forename: Heather N
authors.initials: HN
authors.lastname: Cartwright
authors.affiliation: Section of Cell and Developmental Biology, University of California San Diego, La Jolla, California 92093-0116, USA.
authors.forename: Bing
authors.initials: B
authors.lastname: Yang
authors.affiliation: Section of Cell and Developmental Biology, University of California San Diego, La Jolla, California 92093-0116, USA.
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Bennett
authors.affiliation: Department of Molecular Biology, University of Wyoming, Laramie, Wyoming 82071, USA.
authors.forename: Anne W
authors.initials: AW
authors.lastname: Sylvester
authors.affiliation: Section of Cell and Developmental Biology, University of California San Diego, La Jolla, California 92093-0116, USA.
authors.forename: Laurie G
authors.initials: LG
authors.lastname: Smith