title: Selection of oleaginous yeasts for fatty acid production.
abstract: Oleaginous yeast species are an alternative for the production of lipids or triacylglycerides (TAGs). These yeasts are usually non-pathogenic and able to store TAGs ranging from 20 % to 70 % of their cell mass depending on culture conditions. TAGs originating from oleaginous yeasts can be used as the so-called second generation biofuels, which are based on non-food competing "waste carbon sources".
  
  
  
  In this study the selection of potentially new interesting oleaginous yeast strains is described. Important selection criteria were: a broad maximum temperature and pH range for growth (robustness of the strain), a broad spectrum of carbon sources that can be metabolized (preferably including C-5 sugars), a high total fatty acid content in combination with a low glycogen content and genetic accessibility.
  
  
  
  Based on these selection criteria, among 24 screened species, Schwanniomyces occidentalis (Debaromyces occidentalis) CBS2864 was selected as a promising strain for the production of high amounts of lipids.
authors.affiliation: HAN BioCentre, University of Applied Sciences, P.O. Box 6960, , 6503 GL, Nijmegen, The Netherlands. dennis.lamers@han.nl.|Bioprocess Engineering, Wageningen University and Research Centre, P.O. Box 8129, , 6700 EV, Wageningen, The Netherlands. dennis.lamers@han.nl.
authors.forename: Dennis
authors.initials: D
authors.lastname: Lamers
authors.affiliation: HAN BioCentre, University of Applied Sciences, P.O. Box 6960, , 6503 GL, Nijmegen, The Netherlands.
authors.forename: Nick
authors.initials: N
authors.lastname: van Biezen
authors.affiliation: Bioprocess Engineering, Wageningen University and Research Centre, P.O. Box 8129, , 6700 EV, Wageningen, The Netherlands.
authors.forename: Dirk
authors.initials: D
authors.lastname: Martens
authors.affiliation: HAN BioCentre, University of Applied Sciences, P.O. Box 6960, , 6503 GL, Nijmegen, The Netherlands.
authors.forename: Linda
authors.initials: L
authors.lastname: Peters
authors.affiliation: HAN BioCentre, University of Applied Sciences, P.O. Box 6960, , 6503 GL, Nijmegen, The Netherlands.
authors.forename: Eric
authors.initials: E
authors.lastname: van de Zilver
authors.affiliation: HAN BioCentre, University of Applied Sciences, P.O. Box 6960, , 6503 GL, Nijmegen, The Netherlands.
authors.forename: Nicole
authors.initials: N
authors.lastname: Jacobs-van Dreumel
authors.affiliation: Bioprocess Engineering, Wageningen University and Research Centre, P.O. Box 8129, , 6700 EV, Wageningen, The Netherlands.|University of Nordland, Faculty of Biosciences and Aquaculture, N-8049, Bodø, Norway.
authors.forename: René H
authors.initials: RH
authors.lastname: Wijffels
authors.affiliation: HAN BioCentre, University of Applied Sciences, P.O. Box 6960, , 6503 GL, Nijmegen, The Netherlands.
authors.forename: Christien
authors.initials: C
authors.lastname: Lokman
mesh_terms: D050260:Carbohydrate Metabolism / Q000502:physiology*; D005227:Fatty Acids / Q000096:biosynthesis* / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D009821:Oils / Q000378:metabolism*; D013045:Species Specificity; D013696:Temperature; D015003:Yeasts / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D005227:Fatty Acids; D009821:Oils
keywords: Lipid production; Oleaginous yeast; Schwanniomyces occidentalis; TAG