title: [CHARACTERIZATION OF THE DIFFERENCE IN THE MORPHOLOGY OF DETERGENT RESISTANT MEMBRANES DOMAINS ISOLATED FROM DIFFERENT CELL TYPES BY ATOMIC FORCE MICROSCOPY].
abstract: Planar raft and caveolae are specific membrane clusters with high concentration of cholesterol and lipids with saturated fatty acid. These clasters are resistant to detergents and are denoted as detergent resistant membranes domains (DRMs). Their morphology and size have been studied by atomic force microscopy. The size of planar rafts isolated by Librol from monocytes of healthy volunteers was 150.6 ± 68.6 nm--diameters and 5.7 ± 2.9 nm--height, the size of caveolae was 87.3 ± 46.1 nm--diameters and 9.4 ± 5.4 nm--height. Significant difference have been found morphology and size of DRMs isolated from monocytes of healthy volunteers and patients suffering from myocardial infarction as well as between DRMs isolated from endothelial cells. The study of time-dependent changes in the morphology of isolated planar rafts and caveolae has shown that they quickly aggregate during keeping. Therefore, to asses the actual size and morphology of the DRMS, they should be investigated immediately after isolation.
authors.forename: S N
authors.initials: SN
authors.lastname: Pleskova
authors.forename: E N
authors.initials: EN
authors.lastname: Aybeke
authors.forename: E
authors.initials: E
authors.lastname: Bourillot
authors.forename: E
authors.initials: E
authors.lastname: Lesniewska
mesh_terms: D021941:Caveolae / Q000187:drug effects / Q000648:ultrastructure*; D003902:Detergents / Q000737:chemistry* / Q000633:toxicity; D042783:Endothelial Cells / Q000187:drug effects / Q000648:ultrastructure; D006801:Humans; D008055:Lipids / Q000737:chemistry; D021962:Membrane Microdomains / Q000187:drug effects / Q000648:ultrastructure*; D018625:Microscopy, Atomic Force; D009000:Monocytes / Q000187:drug effects / Q000648:ultrastructure*
chemical_list: D003902:Detergents; D008055:Lipids