title: PprM is necessary for up-regulation of katE1, encoding the major catalase of Deinococcus radiodurans, under unstressed culture conditions.
abstract: Deinococcus radiodurans is a poly-extremophilic organism, capable of tolerating a wide variety of different stresses, such as gamma/ultraviolet radiation, desiccation, and oxidative stress. PprM, a cold shock protein homolog, is involved in the radiation resistance of D. radiodurans, but its role in the oxidative stress response has not been investigated. In this study, we investigated the effect of pprM mutation on catalase gene expression. pprM disruption decreased the mRNA and protein levels of KatE1, which is the major catalase in D. radiodurans, under normal culture conditions. A pprM mutant strain (pprM MT) exhibited decreased catalase activity, and its resistance to hydrogen peroxide (H2O2) decreased accordingly compared with that of the wild-type strain. We confirmed that RecG helicase negatively regulates katE1 under normal culture conditions. Among katE1 transcriptional regulators, the positive regulator drRRA was not altered in pprM (-), while the negative regulators perR, dtxR, and recG were activated more than 2.5-fold in pprM MT. These findings suggest that PprM is necessary for KatE1 production under normal culture conditions by down-regulation of katE1 negative regulators.
authors.affiliation: Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup, 56212, Republic of Korea.|Department of Biological Sciences, College of Biological Sciences and Biotechnology, Chungnam National University, Daejeon, 34134, Republic of Korea.
authors.forename: Sun-Wook
authors.initials: SW
authors.lastname: Jeong
authors.affiliation: Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup, 56212, Republic of Korea.
authors.forename: Ho Seong
authors.initials: HS
authors.lastname: Seo
authors.affiliation: Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup, 56212, Republic of Korea.
authors.forename: Min-Kyu
authors.initials: MK
authors.lastname: Kim
authors.affiliation: Department of Biotechnology and Bioengineering, Chonnam National University, Gwangju, 61186, Republic of Korea.
authors.forename: Jong-Il
authors.initials: JI
authors.lastname: Choi
authors.affiliation: Department of Biological Sciences, College of Biological Sciences and Biotechnology, Chungnam National University, Daejeon, 34134, Republic of Korea. hmlim@cnu.ac.kr.
authors.forename: Heon-Man
authors.initials: HM
authors.lastname: Lim
authors.affiliation: Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup, 56212, Republic of Korea. saylim@kaeri.re.kr.
authors.forename: Sangyong
authors.initials: S
authors.lastname: Lim
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002374:Catalase / Q000235:genetics* / Q000378:metabolism; D057773:Cold Shock Proteins and Peptides / Q000235:genetics / Q000378:metabolism*; D034301:Deinococcus / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D015854:Up-Regulation
chemical_list: D001426:Bacterial Proteins; D057773:Cold Shock Proteins and Peptides; D002374:Catalase
keywords: PprM; ROS; catalase activity; cold-shock protein; katE1