title: Acute Toxicity and Gastroprotection Studies of a New Schiff Base Derived Manganese (II) Complex against HCl/Ethanol-Induced Gastric Ulcerations in Rats.
abstract: Manganese is a crucial element for health. In this study, the gastroprotective efficacy of Mn (II) complex (MDLA) against acidified ethanol (HCl/Ethanol)-induced gastric ulceration in rats was evaluated. The animals were distributed into 5 groups. Groups 1 and 2 received carboxymethylcellulose (CMC), group 3 was pretreated with omeprazole, and groups 4 and 5 were given 10 and 20 mg/kg of MDLA, respectively. After one hour, CMC and HCl/Ethanol were given to groups 2-5 whilst the animals in group 1 were ingested with CMC. After sacrifice, gastric lesions were evaluated by wall mucus, gross appearance, histology, antioxidant enzymes and immunohistochemistry. Group 2 displayed severe gastric damage with a significant reduction in wall mucus. Conversely, gastric lesions were reduced in groups 3-5 by 85.72%, 56.51% and 65.93%, respectively. The rats in groups 3-5 showed up-regulation of heat shock protein 70 (Hsp70) with down-regulation of Bcl-2-associated protein x (Bax). Pretreatment with omeprazole or MDLA led to an increase in the uptake of Periodic Acid Schiff (PAS) stain in the glandular part of the gastric tissue, raised levels of prostaglandin E2 (PGE2) and superoxide dismutase (SOD), and a reduction in malondialdehyde (MDA) concentrations. These results suggested the gastroprotective action of Mn (II) complex.
authors.affiliation: Department of Pharmacy, Faculty of Medicine, University of Malaya 50603 Kuala Lumpur, Malaysia.|Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Mohamed Yousif
authors.initials: MY
authors.lastname: Ibrahim
authors.affiliation: Department of Pharmacy, Faculty of Medicine, University of Malaya 50603 Kuala Lumpur, Malaysia.|Center for Natural Products and Drug Discovery (CENAR), University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Najihah Mohd
authors.initials: NM
authors.lastname: Hashim
authors.affiliation: Department of Microbiology, Faculty of medicine, University of Duhok, 78 Kurdistan, Iraq.
authors.forename: Summaya M
authors.initials: SM
authors.lastname: Dhiyaaldeen
authors.affiliation: Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Mazen M Jamil
authors.initials: MM
authors.lastname: Al-Obaidi
authors.affiliation: Department of Chemistry, Faculty of Science, University of Benghazi, 1308 Benghazi, Libya.
authors.forename: Rashd M
authors.initials: RM
authors.lastname: El-Ferjani
authors.affiliation: School of Pharmacy, Ahfad University for Women (AUW), 167 Omdurman, Sudan.
authors.forename: Hoyam
authors.initials: H
authors.lastname: Adam
authors.affiliation: Department of Chemical Engineering, Faculty of Engineering, Chungbuk National University, Cheongju, Chungbuk 28644, Republic of Korea.
authors.forename: Bassam
authors.initials: B
authors.lastname: Alkotaini
authors.affiliation: Department of Biomedical Science, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.|Institute of Research Management &Monitoring, Deputy Vice Chancellor (Research &Innovation), University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Rami Al
authors.initials: RA
authors.lastname: Batran
authors.affiliation: Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Hapipah Mohd
authors.initials: HM
authors.lastname: Ali
mesh_terms: D000818:Animals; D000897:Anti-Ulcer Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000633:toxicity*; D000431:Ethanol / Q000008:administration & dosage; D005260:Female; D005753:Gastric Mucosa / Q000187:drug effects; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D008345:Manganese / Q000008:administration & dosage* / Q000737:chemistry / Q000633:toxicity*; D009853:Omeprazole / Q000008:administration & dosage; D017207:Rats, Sprague-Dawley; D012545:Schiff Bases; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy*; D023382:Toxicity Tests, Acute
chemical_list: D000897:Anti-Ulcer Agents; D012545:Schiff Bases; D000431:Ethanol; D008345:Manganese; D009853:Omeprazole