title: T cell-derived interleukin-10 is an important regulator of the Th17 response during lethal alphavirus encephalomyelitis.
abstract: Neuroadapted Sindbis virus infection of mice causes T cell-mediated fatal encephalomyelitis. In the absence of IL-10, pathogenic Th17 cells are increased and disease is accelerated. Lymphoid and myeloid cell contributions to IL-10 production were determined using VertX IL-10 transcriptional eGFP reporter mice. Effector and regulatory CD4(+) and CD8(+) T cells in the brain, but not the cervical lymph nodes, were the primary producers of IL-10. Th17 and Th1/Th17 cells were increased in mice that lacked T cell IL-10 production, although less than in the absence of IL-10. Morbidity and mortality were not affected suggesting an IL-10 threshold for disease exacerbation.
authors.affiliation: Program in Cellular and Molecular Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA; W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, 21205, USA.
authors.forename: Kirsten A
authors.initials: KA
authors.lastname: Kulcsar
authors.affiliation: Program in Cellular and Molecular Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA; W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, 21205, USA. Electronic address: dgriffi6@jhu.edu.
authors.forename: Diane E
authors.initials: DE
authors.lastname: Griffin
mesh_terms: D000524:Alphavirus / Q000472:pathogenicity; D018354:Alphavirus Infections / Q000235:genetics / Q000276:immunology* / Q000473:pathology*; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D002490:Central Nervous System / Q000473:pathology / Q000821:virology; D004195:Disease Models, Animal; D004679:Encephalomyelitis / Q000235:genetics / Q000276:immunology* / Q000473:pathology* / Q000821:virology; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018709:Statistics, Nonparametric; D058504:Th17 Cells / Q000378:metabolism* / Q000473:pathology
chemical_list: D015703:Antigens, CD; C556062:IL10 protein, mouse; C485184:enhanced green fluorescent protein; D016753:Interleukin-10; D049452:Green Fluorescent Proteins
keywords: IL-10 source; Immunopathology; Mice; Sindbis virus; Th17