title: MicroRNA-182 aggravates cerebral ischemia injury by targeting inhibitory member of the ASPP family (iASPP).
abstract: Ischemic stroke is one of the leading causes of death and disability globally and has been regarded as a major public health problem. MicroRNA 182 (miR-182) plays important roles in cellular differentiation, cell growth, cell apoptosis and metastasis. However, the role of miR-182 in the cerebral ischemia injury has never been investigated. In the present study, we demonstrate a crucial role of miR-182 in down-regulating inhibitory member of the ASPP family (iASPP) expression and promoting cerebral ischemia injury. MiR-182 also promotes NO and 3-NT production, and Caspase3 expression, while reduces SOD and MnSOD activities. Furthermore, the amplified cerebral ischemia injury induced by miR-182 is aggravated by inhibition of iASPP. In conclusion, miR-182 plays an aggressive role in the cerebral ischemia injury, and this is associated with inhibited iASPP expression.
authors.affiliation: Department of Anesthesia, The First Affiliated Hospital of University of South China, China.
authors.forename: Han
authors.initials: H
authors.lastname: Yi
authors.affiliation: Department of Internal Medicine, The Second Xiangya Hospital of Central South University, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Anesthesia, The First Affiliated Hospital of University of South China, China.
authors.forename: Fengrui
authors.initials: F
authors.lastname: Yang
authors.affiliation: Department of Anesthesia, The First Affiliated Hospital of University of South China, China.
authors.forename: Wenzhi
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Anesthesia, The First Affiliated Hospital of University of South China, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: He
authors.affiliation: Department of Anesthesia, The First Affiliated Hospital of University of South China, China. Electronic address: huxiaoling0734@126.com.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Hu
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism* / Q000473:pathology; D053148:Caspase 3 / Q000096:biosynthesis; D002460:Cell Line; D005786:Gene Expression Regulation; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism; D012097:Repressor Proteins / Q000096:biosynthesis*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D035683:MicroRNAs; C540524:Mirn182 microRNA, mouse; D012097:Repressor Proteins; D009569:Nitric Oxide; D013482:Superoxide Dismutase; C505409:Casp3 protein, mouse; D053148:Caspase 3
keywords: Cerebral ischemia/reperfusion injury; MCAO reperfusion; Nitric oxide; Oxidative stress; iASPP; miR-182