title: Degradation of polynuclear aromatic hydrocarbons by two strains of Pseudomonas.
abstract: The goal of this investigation was to isolate competent polynuclear aromatic hydrocarbons degraders that can utilize polynuclear aromatic hydrocarbons of former industrial sites at McDoel Switchyard in Bloomington, Indiana. Using conventional enrichment method based on soil slurry, we isolated, screened and purified two bacterial species strains PB1 and PB2. Applying the ribotyping technique using the 16S rRNA gene analysis, the strains were assigned to the genus Pseudomonas (Pseudomonas plecoglossicida strain PB1 and Pseudomonas sp. PB2). Both isolates showed promising metabolic capacity on pyrene sprayed MS agar plates during the preliminary investigations. Using time course studies in the liquid cultures at calculated concentrations 123, 64, 97 and 94ppm for naphthalene, chrysene, fluroanthene and pyrene, P. plecoglossicida strain PB1 and Pseudomonas sp. PB2 showed partial utilization of the polynuclear aromatic hydrocarbons. Naphthalene was degraded between 26% and 40%, chrysene 14% and 16%, fluroanthene 5% and 7%; pyrene 8% and 13% by P. plecoglossicida strain PB1 and Pseudomonas sp. PB2 respectively. Based on their growth profile, we developed a model R(2)=1 to predict the degradation rate of slow polynuclear aromatic hydrocarbon-degraders where all the necessary parameters are constant. From this investigation, we confirm that the former industrial site soil microbial communities may be explored for the biorestoration of the industrial site.
authors.affiliation: Department of Biological Sciences, School of Natural and Applied Sciences, College of Science and Technology, Covenant University, Canaan Land, Ota, Ogun State, Nigeria; Department of Biotechnology and Food technology, University of Johannesburg, Doornfontein, Johannesburg, South Africa. Electronic address: nwinyiobinna@gmail.com.
authors.forename: Obinna C
authors.initials: OC
authors.lastname: Nwinyi
authors.affiliation: Department of Mechanical Engineering, College of Science and Technology, Covenant University, Canaan Land, Ota, Ogun State, Nigeria.
authors.forename: Oluseyi O
authors.initials: OO
authors.lastname: Ajayi
authors.affiliation: Department of Microbiology, University of Lagos, Lagos, Nigeria.
authors.forename: Olukayode O
authors.initials: OO
authors.lastname: Amund
mesh_terms: D001673:Biodegradation, Environmental; D002244:Carbon / Q000737:chemistry; D002911:Chrysenes / Q000378:metabolism; D006859:Hydrogen / Q000737:chemistry; D009281:Naphthalenes / Q000378:metabolism; D009584:Nitrogen / Q000737:chemistry; D010802:Phylogeny; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism*; D011549:Pseudomonas / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D011721:Pyrenes / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology
chemical_list: D002911:Chrysenes; D009281:Naphthalenes; D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes; D012336:RNA, Ribosomal, 16S; D012987:Soil; C031180:chrysene; C031721:naphthalene; D002244:Carbon; D006859:Hydrogen; C030984:pyrene; D009584:Nitrogen
keywords: 16S rRNA gene analysis; Competent PAH degraders; Former industrial sites; Soil microbial communities