title: Olfactory receptor for prostaglandin F2α mediates male fish courtship behavior.
abstract: Pheromones play vital roles for survival and reproduction in various organisms. In many fishes, prostaglandin F2α acts not only as a female reproductive hormone, facilitating ovulation and spawning, but also as a sex pheromone inducing male reproductive behaviors. Here, we unravel the molecular and neural circuit mechanisms underlying the pheromonal action of prostaglandin F2α in zebrafish. Prostaglandin F2α specifically activates two olfactory receptors with different sensitivities and expression in distinct populations of ciliated olfactory sensory neurons. Pheromone information is then transmitted to two ventromedial glomeruli in the olfactory bulb and further to four regions in higher olfactory centers. Mutant male zebrafish deficient in the high-affinity receptor exhibit loss of attractive response to prostaglandin F2α and impairment of courtship behaviors toward female fish. These findings demonstrate the functional significance and activation of selective neural circuitry for the sex pheromone prostaglandin F2α and its cognate olfactory receptor in fish reproductive behavior.
authors.affiliation: RIKEN Brain Science Institute, Saitama, Japan.|Department of Bioengineering, Nagaoka University of Technology, Niigata, Japan.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Yabuki
authors.affiliation: RIKEN Brain Science Institute, Saitama, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Koide
authors.affiliation: RIKEN Brain Science Institute, Saitama, Japan.
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Miyasaka
authors.affiliation: RIKEN Brain Science Institute, Saitama, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Wakisaka
authors.affiliation: RIKEN Brain Science Institute, Saitama, Japan.|ERATO Touhara Chemosensory Signal Project, JST, The University of Tokyo, Tokyo, Japan.
authors.forename: Miwa
authors.initials: M
authors.lastname: Masuda
authors.affiliation: Brain Science Institute, Saitama University, Saitama, Japan.
authors.forename: Masamichi
authors.initials: M
authors.lastname: Ohkura
authors.affiliation: Brain Science Institute, Saitama University, Saitama, Japan.
authors.forename: Junichi
authors.initials: J
authors.lastname: Nakai
authors.affiliation: Department of Pharmaceutical Biochemistry, Kumamoto University Graduate School of Pharmaceutical Sciences, Kumamoto, Japan.
authors.forename: Kyoshiro
authors.initials: K
authors.lastname: Tsuge
authors.affiliation: Department of Pharmaceutical Biochemistry, Kumamoto University Graduate School of Pharmaceutical Sciences, Kumamoto, Japan.
authors.forename: Soken
authors.initials: S
authors.lastname: Tsuchiya
authors.affiliation: Department of Pharmaceutical Biochemistry, Kumamoto University Graduate School of Pharmaceutical Sciences, Kumamoto, Japan.
authors.forename: Yukihiko
authors.initials: Y
authors.lastname: Sugimoto
authors.affiliation: RIKEN Brain Science Institute, Saitama, Japan.|Department of Bioengineering, Nagaoka University of Technology, Niigata, Japan.|ERATO Touhara Chemosensory Signal Project, JST, The University of Tokyo, Tokyo, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Yoshihara
mesh_terms: D000818:Animals; D003380:Courtship; D015237:Dinoprost / Q000378:metabolism; D009830:Olfactory Bulb / Q000187:drug effects / Q000378:metabolism*; D018034:Olfactory Receptor Neurons / Q000378:metabolism*; D010675:Pheromones / Q000378:metabolism; D018035:Receptors, Odorant / Q000378:metabolism*; D011982:Receptors, Prostaglandin / Q000378:metabolism*; D012098:Reproduction / Q000502:physiology; D012726:Sexual Behavior, Animal / Q000502:physiology; D012903:Smell / Q000502:physiology*; D015027:Zebrafish
chemical_list: D010675:Pheromones; D018035:Receptors, Odorant; D011982:Receptors, Prostaglandin; C034671:prostaglandin F2alpha receptor; D015237:Dinoprost