title: Distinct functional responses to stressors of bone marrow derived dendritic cells from diverse inbred chicken lines.
abstract: Differences in responses of chicken bone marrow derived dendritic cells (BMDC) to in vitro treatment with lipopolysaccharide (LPS), heat, and LPS + heat were identified. The Fayoumi is more disease resistant and heat tolerant than the Leghorn line. Nitric Oxide (NO) production, phagocytic ability, MHC II surface expression and mRNA expression were measured. NO was induced in BMDC from both lines in response to LPS and LPS + heat stimulation; Fayoumi produced more NO with LPS treatment. Fayoumi had higher phagocytic ability and MHC II surface expression. Gene expression for the heat-related genes BAG3, HSP25, HSPA2, and HSPH1 was strongly induced with heat and few differences existed between lines. Expression for the immune-related genes CCL4, CCL5, CD40, GM-CSF, IFN-γ, IL-10, IL-12β, IL-1β, IL-6, IL-8, and iNOS was highly induced in response to LPS and different between lines. This research contributes to the sparse knowledge of genetic differences in chicken BMDC biology and function.
authors.affiliation: Department of Animal Science, Iowa State University, Ames, IA, USA.
authors.forename: Angelica
authors.initials: A
authors.lastname: Van Goor
authors.affiliation: Department of Animal Science, Iowa State University, Ames, IA, USA; Department of Animal Biochemistry and Biotechnology, UTP University of Science and Technology, Bydgoszcz, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Slawinska
authors.affiliation: Department of Animal and Food Sciences, University of Delaware, Newark, DE, USA.
authors.forename: Carl J
authors.initials: CJ
authors.lastname: Schmidt
authors.affiliation: Department of Animal Science, Iowa State University, Ames, IA, USA. Electronic address: sjlamont@iastate.edu.
authors.forename: Susan J
authors.initials: SJ
authors.lastname: Lamont
mesh_terms: D000818:Animals; D017849:Animals, Inbred Strains; D030161:Avian Proteins / Q000378:metabolism; D001854:Bone Marrow Cells / Q000276:immunology*; D002478:Cells, Cultured; D002645:Chickens / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D004198:Disease Susceptibility; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D006358:Hot Temperature / Q000009:adverse effects; D007109:Immunity; D008070:Lipopolysaccharides / Q000276:immunology; D009569:Nitric Oxide / Q000378:metabolism; D010587:Phagocytosis; D013312:Stress, Physiological / Q000276:immunology*
chemical_list: D030161:Avian Proteins; D000949:Histocompatibility Antigens Class II; D008070:Lipopolysaccharides; D009569:Nitric Oxide
keywords: Dendritic cells; Fayoumi; Gene expression; Leghorn; MHC-II; Nitric oxide