title: Functional Expression of FSH Receptor in Endometriotic Lesions.
abstract: FSH receptor (FSHR), besides being expressed in gonads, is also expressed in some extragonadal tissues at low levels.
  
  
  
  We examined the functional expression of FSHR in different types of endometriotic lesions.
  
  
  
  Extensive studies were carried out to detect functional FSHR expression and FSH-stimulated estrogen production in ovarian endometriomas and recto-vaginal endometriotic nodules (RVEN). Normal endometrium, ovary, and myometrium tissues from nonpregnant cycling women served as controls.
  
  
  
  This laboratory-based study was carried out on tissue specimens from patients with endometriosis and healthy donors.
  
  
  
  Endometriotic lesions and normal secretory-phase endometrium showed FSHR expression at both mRNA and protein level. RVEN and ovarian endometrioma demonstrated up-regulated CYP19A1, dependent on the activation of CYP19A1 proximal promoter II. Estrogen receptor-β (ESR2) expression was significantly increased in RVEN vs normal endometrium. Recombinant human FSH stimulation of RVEN explants significantly increased estradiol production and CYP19A1 and ESR2 expression. FSHR was up-regulated in recombinant human FSH-stimulated endometrial and decidualized stromal cells with increased CYP19A1 expression.
  
  
  
  We described a novel functional FSHR expression, where FSH-stimulated CYP19A1 expression and estrogen production in RVEN are demonstrated. This locally FSH-induced estrogen production may contribute to the pathology, development, progression, and severity of RVEN.
authors.affiliation: Department of Biology and Pathology of Human Reproduction (D.P.-T., M.S., S.W.), Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748 Olsztyn, Poland; Department of Physiology (M.C., M.D., I.H., N.A.R.), Institute of Biomedicine, University of Turku, 20520 Turku, Finland; Department of Hormonal Action Mechanisms (M.C.), Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10748 Olsztyn, Poland; Department of Reproduction and Gynecological Endocrinology (J.S., I.S., S.W., N.A.R.), Medical University of Bialystok, 15-276 Bialystok, Poland; Department of Pathomorphology (P.B.), Medical University of Bialystok, 15269 Bialystok, Poland; Tomaszewski Medical Center of Obstetrics and Gynecology (J.To.), 15-224 Bialystok, Poland; Department of Obstetrics and Gynecology (J.Ta.), University of Helsinki and Helsinki University Hospital, 00029 Helsinki, Finland; and Institute of Reproductive and Developmental Biology (I.H.), Imperial College London, W12 0NN London, United Kingdom.
authors.forename: Donata
authors.initials: D
authors.lastname: Ponikwicka-Tyszko
authors.affiliation: Department of Biology and Pathology of Human Reproduction (D.P.-T., M.S., S.W.), Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748 Olsztyn, Poland; Department of Physiology (M.C., M.D., I.H., N.A.R.), Institute of Biomedicine, University of Turku, 20520 Turku, Finland; Department of Hormonal Action Mechanisms (M.C.), Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10748 Olsztyn, Poland; Department of Reproduction and Gynecological Endocrinology (J.S., I.S., S.W., N.A.R.), Medical University of Bialystok, 15-276 Bialystok, Poland; Department of Pathomorphology (P.B.), Medical University of Bialystok, 15269 Bialystok, Poland; Tomaszewski Medical Center of Obstetrics and Gynecology (J.To.), 15-224 Bialystok, Poland; Department of Obstetrics and Gynecology (J.Ta.), University of Helsinki and Helsinki University Hospital, 00029 Helsinki, Finland; and Institute of Reproductive and Developmental Biology (I.H.), Imperial College London, W12 0NN London, United Kingdom.
authors.forename: Marcin
authors.initials: M
authors.lastname: Chrusciel
authors.affiliation: Department of Biology and Pathology of Human Reproduction (D.P.-T., M.S., S.W.), Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748 Olsztyn, Poland; Department of Physiology (M.C., M.D., I.H., N.A.R.), Institute of Biomedicine, University of Turku, 20520 Turku, Finland; Department of Hormonal Action Mechanisms (M.C.), Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10748 Olsztyn, Poland; Department of Reproduction and Gynecological Endocrinology (J.S., I.S., S.W., N.A.R.), Medical University of Bialystok, 15-276 Bialystok, Poland; Department of Pathomorphology (P.B.), Medical University of Bialystok, 15269 Bialystok, Poland; Tomaszewski Medical Center of Obstetrics and Gynecology (J.To.), 15-224 Bialystok, Poland; Department of Obstetrics and Gynecology (J.Ta.), University of Helsinki and Helsinki University Hospital, 00029 Helsinki, Finland; and Institute of Reproductive and Developmental Biology (I.H.), Imperial College London, W12 0NN London, United Kingdom.
authors.forename: Joanna
authors.initials: J
authors.lastname: Stelmaszewska
authors.affiliation: Department of Biology and Pathology of Human Reproduction (D.P.-T., M.S., S.W.), Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748 Olsztyn, Poland; Department of Physiology (M.C., M.D., I.H., N.A.R.), Institute of Biomedicine, University of Turku, 20520 Turku, Finland; Department of Hormonal Action Mechanisms (M.C.), Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10748 Olsztyn, Poland; Department of Reproduction and Gynecological Endocrinology (J.S., I.S., S.W., N.A.R.), Medical University of Bialystok, 15-276 Bialystok, Poland; Department of Pathomorphology (P.B.), Medical University of Bialystok, 15269 Bialystok, Poland; Tomaszewski Medical Center of Obstetrics and Gynecology (J.To.), 15-224 Bialystok, Poland; Department of Obstetrics and Gynecology (J.Ta.), University of Helsinki and Helsinki University Hospital, 00029 Helsinki, Finland; and Institute of Reproductive and Developmental Biology (I.H.), Imperial College London, W12 0NN London, United Kingdom.
authors.forename: Piotr
authors.initials: P
authors.lastname: Bernaczyk
authors.affiliation: Department of Biology and Pathology of Human Reproduction (D.P.-T., M.S., S.W.), Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748 Olsztyn, Poland; Department of Physiology (M.C., M.D., I.H., N.A.R.), Institute of Biomedicine, University of Turku, 20520 Turku, Finland; Department of Hormonal Action Mechanisms (M.C.), Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10748 Olsztyn, Poland; Department of Reproduction and Gynecological Endocrinology (J.S., I.S., S.W., N.A.R.), Medical University of Bialystok, 15-276 Bialystok, Poland; Department of Pathomorphology (P.B.), Medical University of Bialystok, 15269 Bialystok, Poland; Tomaszewski Medical Center of Obstetrics and Gynecology (J.To.), 15-224 Bialystok, Poland; Department of Obstetrics and Gynecology (J.Ta.), University of Helsinki and Helsinki University Hospital, 00029 Helsinki, Finland; and Institute of Reproductive and Developmental Biology (I.H.), Imperial College London, W12 0NN London, United Kingdom.
authors.forename: Maria
authors.initials: M
authors.lastname: Sztachelska
authors.affiliation: Department of Biology and Pathology of Human Reproduction (D.P.-T., M.S., S.W.), Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748 Olsztyn, Poland; Department of Physiology (M.C., M.D., I.H., N.A.R.), Institute of Biomedicine, University of Turku, 20520 Turku, Finland; Department of Hormonal Action Mechanisms (M.C.), Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10748 Olsztyn, Poland; Department of Reproduction and Gynecological Endocrinology (J.S., I.S., S.W., N.A.R.), Medical University of Bialystok, 15-276 Bialystok, Poland; Department of Pathomorphology (P.B.), Medical University of Bialystok, 15269 Bialystok, Poland; Tomaszewski Medical Center of Obstetrics and Gynecology (J.To.), 15-224 Bialystok, Poland; Department of Obstetrics and Gynecology (J.Ta.), University of Helsinki and Helsinki University Hospital, 00029 Helsinki, Finland; and Institute of Reproductive and Developmental Biology (I.H.), Imperial College London, W12 0NN London, United Kingdom.
authors.forename: Iwona
authors.initials: I
authors.lastname: Sidorkiewicz
authors.affiliation: Department of Biology and Pathology of Human Reproduction (D.P.-T., M.S., S.W.), Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748 Olsztyn, Poland; Department of Physiology (M.C., M.D., I.H., N.A.R.), Institute of Biomedicine, University of Turku, 20520 Turku, Finland; Department of Hormonal Action Mechanisms (M.C.), Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10748 Olsztyn, Poland; Department of Reproduction and Gynecological Endocrinology (J.S., I.S., S.W., N.A.R.), Medical University of Bialystok, 15-276 Bialystok, Poland; Department of Pathomorphology (P.B.), Medical University of Bialystok, 15269 Bialystok, Poland; Tomaszewski Medical Center of Obstetrics and Gynecology (J.To.), 15-224 Bialystok, Poland; Department of Obstetrics and Gynecology (J.Ta.), University of Helsinki and Helsinki University Hospital, 00029 Helsinki, Finland; and Institute of Reproductive and Developmental Biology (I.H.), Imperial College London, W12 0NN London, United Kingdom.
authors.forename: Milena
authors.initials: M
authors.lastname: Doroszko
authors.affiliation: Department of Biology and Pathology of Human Reproduction (D.P.-T., M.S., S.W.), Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748 Olsztyn, Poland; Department of Physiology (M.C., M.D., I.H., N.A.R.), Institute of Biomedicine, University of Turku, 20520 Turku, Finland; Department of Hormonal Action Mechanisms (M.C.), Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10748 Olsztyn, Poland; Department of Reproduction and Gynecological Endocrinology (J.S., I.S., S.W., N.A.R.), Medical University of Bialystok, 15-276 Bialystok, Poland; Department of Pathomorphology (P.B.), Medical University of Bialystok, 15269 Bialystok, Poland; Tomaszewski Medical Center of Obstetrics and Gynecology (J.To.), 15-224 Bialystok, Poland; Department of Obstetrics and Gynecology (J.Ta.), University of Helsinki and Helsinki University Hospital, 00029 Helsinki, Finland; and Institute of Reproductive and Developmental Biology (I.H.), Imperial College London, W12 0NN London, United Kingdom.
authors.forename: Jakub
authors.initials: J
authors.lastname: Tomaszewski
authors.affiliation: Department of Biology and Pathology of Human Reproduction (D.P.-T., M.S., S.W.), Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748 Olsztyn, Poland; Department of Physiology (M.C., M.D., I.H., N.A.R.), Institute of Biomedicine, University of Turku, 20520 Turku, Finland; Department of Hormonal Action Mechanisms (M.C.), Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10748 Olsztyn, Poland; Department of Reproduction and Gynecological Endocrinology (J.S., I.S., S.W., N.A.R.), Medical University of Bialystok, 15-276 Bialystok, Poland; Department of Pathomorphology (P.B.), Medical University of Bialystok, 15269 Bialystok, Poland; Tomaszewski Medical Center of Obstetrics and Gynecology (J.To.), 15-224 Bialystok, Poland; Department of Obstetrics and Gynecology (J.Ta.), University of Helsinki and Helsinki University Hospital, 00029 Helsinki, Finland; and Institute of Reproductive and Developmental Biology (I.H.), Imperial College London, W12 0NN London, United Kingdom.
authors.forename: Juha S
authors.initials: JS
authors.lastname: Tapanainen
authors.affiliation: Department of Biology and Pathology of Human Reproduction (D.P.-T., M.S., S.W.), Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748 Olsztyn, Poland; Department of Physiology (M.C., M.D., I.H., N.A.R.), Institute of Biomedicine, University of Turku, 20520 Turku, Finland; Department of Hormonal Action Mechanisms (M.C.), Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10748 Olsztyn, Poland; Department of Reproduction and Gynecological Endocrinology (J.S., I.S., S.W., N.A.R.), Medical University of Bialystok, 15-276 Bialystok, Poland; Department of Pathomorphology (P.B.), Medical University of Bialystok, 15269 Bialystok, Poland; Tomaszewski Medical Center of Obstetrics and Gynecology (J.To.), 15-224 Bialystok, Poland; Department of Obstetrics and Gynecology (J.Ta.), University of Helsinki and Helsinki University Hospital, 00029 Helsinki, Finland; and Institute of Reproductive and Developmental Biology (I.H.), Imperial College London, W12 0NN London, United Kingdom.
authors.forename: Ilpo
authors.initials: I
authors.lastname: Huhtaniemi
authors.affiliation: Department of Biology and Pathology of Human Reproduction (D.P.-T., M.S., S.W.), Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748 Olsztyn, Poland; Department of Physiology (M.C., M.D., I.H., N.A.R.), Institute of Biomedicine, University of Turku, 20520 Turku, Finland; Department of Hormonal Action Mechanisms (M.C.), Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10748 Olsztyn, Poland; Department of Reproduction and Gynecological Endocrinology (J.S., I.S., S.W., N.A.R.), Medical University of Bialystok, 15-276 Bialystok, Poland; Department of Pathomorphology (P.B.), Medical University of Bialystok, 15269 Bialystok, Poland; Tomaszewski Medical Center of Obstetrics and Gynecology (J.To.), 15-224 Bialystok, Poland; Department of Obstetrics and Gynecology (J.Ta.), University of Helsinki and Helsinki University Hospital, 00029 Helsinki, Finland; and Institute of Reproductive and Developmental Biology (I.H.), Imperial College London, W12 0NN London, United Kingdom.
authors.forename: Slawomir
authors.initials: S
authors.lastname: Wolczynski
authors.affiliation: Department of Biology and Pathology of Human Reproduction (D.P.-T., M.S., S.W.), Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748 Olsztyn, Poland; Department of Physiology (M.C., M.D., I.H., N.A.R.), Institute of Biomedicine, University of Turku, 20520 Turku, Finland; Department of Hormonal Action Mechanisms (M.C.), Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10748 Olsztyn, Poland; Department of Reproduction and Gynecological Endocrinology (J.S., I.S., S.W., N.A.R.), Medical University of Bialystok, 15-276 Bialystok, Poland; Department of Pathomorphology (P.B.), Medical University of Bialystok, 15269 Bialystok, Poland; Tomaszewski Medical Center of Obstetrics and Gynecology (J.To.), 15-224 Bialystok, Poland; Department of Obstetrics and Gynecology (J.Ta.), University of Helsinki and Helsinki University Hospital, 00029 Helsinki, Finland; and Institute of Reproductive and Developmental Biology (I.H.), Imperial College London, W12 0NN London, United Kingdom.
authors.forename: Nafis A
authors.initials: NA
authors.lastname: Rahman
mesh_terms: D000328:Adult; D001141:Aromatase / Q000235:genetics* / Q000378:metabolism; D016022:Case-Control Studies; D004715:Endometriosis / Q000235:genetics* / Q000473:pathology; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D004958:Estradiol / Q000378:metabolism; D047629:Estrogen Receptor beta / Q000502:physiology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D010049:Ovarian Diseases / Q000235:genetics / Q000473:pathology; D011401:Promoter Regions, Genetic / Q000187:drug effects; D011962:Receptors, FSH / Q000235:genetics* / Q000378:metabolism; D012002:Rectal Diseases / Q000235:genetics* / Q000473:pathology; D014623:Vaginal Diseases / Q000235:genetics* / Q000473:pathology; D055815:Young Adult
chemical_list: D047629:Estrogen Receptor beta; D011962:Receptors, FSH; D004958:Estradiol; D005640:Follicle Stimulating Hormone; D001141:Aromatase; C561410:CYP19A1 protein, human