title: Maternal food restriction in rats of the F0generation increases retroperitoneal fat, the number and size of adipocytes and induces periventricular astrogliosis in female F1and male F2generations.
abstract: The present study investigated whether male offspring (F2generation) from female rats (F1generation) whose mothers (F0generation) were food restricted during gestation inherit a phenotypic transgenerational tendency towards being overweight and obese in the juvenile period, in the absence of food restriction in the F1/F2generations. Dams of the F0generation were 40% food restricted during pregnancy. Bodyweight, the number and size of larger and small hypodermal adipocytes (HAs), total retroperitoneal fat (RPF) weight and the expression of glial fibrillary acidic protein (GFAP) in periventricular hypothalamic astrocytes (PHAs), as determined by immunohistochemistry, were evaluated in both generations. In the female F1generation, there was low bodyweight gain only during the juvenile period (30-65 days of age), a decrease in the size of small adipocytes, an increase in the number of small adipocytes, an increase in RPF weight and an increase in GFAP expression in PHAs at 90-95 days of age. In males of the F2generation at 50 days of age, there was increased bodyweight and RPF weight, and a small number of adipocytes and GFAP expression in PHAs. These data indicate that the phenotypic transgenerational tendency towards being overweight and obese was observed in females (F1) from mothers (F0) that were prenatally food restricted was transmitted to their male offspring.
authors.affiliation: Environmental and Experimental Pathology, Paulista University, UNIP, Rua Dr Bacelar, 1212, São Paulo, SP, 04026-002, Brazil.
authors.forename: A O
authors.initials: AO
authors.lastname: Joaquim
authors.affiliation: Environmental and Experimental Pathology, Paulista University, UNIP, Rua Dr Bacelar, 1212, São Paulo, SP, 04026-002, Brazil.
authors.forename: C P
authors.initials: CP
authors.lastname: Coelho
authors.affiliation: Environmental and Experimental Pathology, Paulista University, UNIP, Rua Dr Bacelar, 1212, São Paulo, SP, 04026-002, Brazil.
authors.forename: P Dias
authors.initials: PD
authors.lastname: Motta
authors.affiliation: Department of Pathology, School of Veterinary Medicine, University of São Paulo, Av. Prof. Dr Orlando Marques de Paiva, 87, Sao Paulo, SP, 05508-270, Brazil.
authors.forename: L F
authors.initials: LF
authors.lastname: Felício
authors.affiliation: Environmental and Experimental Pathology, Paulista University, UNIP, Rua Dr Bacelar, 1212, São Paulo, SP, 04026-002, Brazil.
authors.forename: E F
authors.initials: EF
authors.lastname: Bondan
authors.affiliation: Mathematics, Computing and Cognition Center, Federal University of ABC, Av. dos Estados, 5001, Santo André, SP, 09210-971, Brazil.
authors.forename: E
authors.initials: E
authors.lastname: Teodorov
authors.affiliation: Environmental and Experimental Pathology, Paulista University, UNIP, Rua Dr Bacelar, 1212, São Paulo, SP, 04026-002, Brazil.
authors.forename: M F M
authors.initials: MFM
authors.lastname: Martins
authors.affiliation: Environmental and Experimental Pathology, Paulista University, UNIP, Rua Dr Bacelar, 1212, São Paulo, SP, 04026-002, Brazil.
authors.forename: T B
authors.initials: TB
authors.lastname: Kirsten
authors.affiliation: Environmental and Experimental Pathology, Paulista University, UNIP, Rua Dr Bacelar, 1212, São Paulo, SP, 04026-002, Brazil.
authors.forename: L V
authors.initials: LV
authors.lastname: Bonamin
authors.affiliation: Environmental and Experimental Pathology, Paulista University, UNIP, Rua Dr Bacelar, 1212, São Paulo, SP, 04026-002, Brazil.
authors.forename: M M
authors.initials: MM
authors.lastname: Bernardi
mesh_terms: D052438:Adipocytes, White / Q000473:pathology; D000818:Animals; D001253:Astrocytes / Q000473:pathology*; D002452:Cell Count; D048429:Cell Size; D003433:Crosses, Genetic; D005260:Female; D005508:Food Deprivation; D007031:Hypothalamus / Q000473:pathology; D050152:Intra-Abdominal Fat / Q000473:pathology*; D008297:Male; D044342:Malnutrition / Q000150:complications* / Q000235:genetics / Q000473:pathology; D011247:Pregnancy; D011248:Pregnancy Complications / Q000235:genetics / Q000473:pathology*; D011297:Prenatal Exposure Delayed Effects / Q000235:genetics / Q000473:pathology*; D039401:Prenatal Nutritional Physiological Phenomena; D051381:Rats; D017208:Rats, Wistar