title: Biocompatible polyhydroxyalkanoates/bacterial cellulose composites: Preparation, characterization, and in vitro evaluation.
abstract: Biocompatible composites play a critical role as scaffolds in tissue engineering. Novel biocomposites made from poly(3-hydroxybutyrate) (PHB), polyhydroxyalkanoate (PHA) and bacterial cellulose (BC) in different concentrations were prepared by solution casting and their thermal and mechanical behavior as well as biocompatibility was characterized. BC addition increased the thermal stability of the polymer matrix as evidenced by thermogravimetric analysis. The crystallinity of PHB and the crystallization temperature decreased with the addition of BC and PHA, thus increasing the processing window. BC in small concentration determined an increase in the mechanical properties due to a concerted action of PHA and filler. Good cells attachment and proliferation were observed for all the biocomposites. By the addition of PHA (more hydrophobic than the matrix) and various amounts of BC (highly hydrophilic), surface properties and cell attachment can be controlled. Cytocompatibility studies using L929 cell line revealed that this material is suitable for biomedical applications. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2576-2584, 2016.
authors.affiliation: Department of Polymer, National Institute for R&D in Chemistry and Petrochemistry ICECHIM, Bucharest, Romania.
authors.forename: Ioana
authors.initials: I
authors.lastname: Chiulan
authors.affiliation: Department of Polymer, National Institute for R&D in Chemistry and Petrochemistry ICECHIM, Bucharest, Romania.
authors.forename: Denis
authors.initials: D
authors.lastname: Mihaela Panaitescu
authors.affiliation: Department of Polymer, National Institute for R&D in Chemistry and Petrochemistry ICECHIM, Bucharest, Romania.
authors.forename: Adriana
authors.initials: A
authors.lastname: Nicoleta Frone
authors.affiliation: Department of Bioresources and Polymer Science, Faculty of Applied Chemistry and Materials Science, Polytechnic University of Bucharest, Bucharest, Romania.
authors.forename: Mircea
authors.initials: M
authors.lastname: Teodorescu
authors.affiliation: Department of Polymer, National Institute for R&D in Chemistry and Petrochemistry ICECHIM, Bucharest, Romania.
authors.forename: Cristian
authors.initials: C
authors.lastname: Andi Nicolae
authors.affiliation: Department of Pharmaceutical Biotechnologies, National Institute for Chemical Pharmaceutical R&D ICCF, Bucharest, Romania.
authors.forename: Angela
authors.initials: A
authors.lastname: Căşărică
authors.affiliation: Infection and Immunity Laboratory, Cantacuzino National Institute, Bucharest, Romania.
authors.forename: Vlad
authors.initials: V
authors.lastname: Tofan
authors.affiliation: Infection and Immunity Laboratory, Cantacuzino National Institute, Bucharest, Romania.
authors.forename: Aurora
authors.initials: A
authors.lastname: Sălăgeanu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D002482:Cellulose / Q000031:analogs & derivatives*; D055119:Elastic Modulus; D041822:Gluconacetobacter xylinus / Q000737:chemistry; D006885:Hydroxybutyrates / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D011091:Polyesters / Q000737:chemistry*; D054813:Polyhydroxyalkanoates / Q000737:chemistry*; D013696:Temperature; D023822:Tissue Engineering; D000094122:Polyhydroxybutyrates
chemical_list: D001672:Biocompatible Materials; D002482:Cellulose; D006885:Hydroxybutyrates; D011091:Polyesters; D054813:Polyhydroxyalkanoates; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates
keywords: bacterial cellulose; cell viability; poly(3-hydroxybutyrate); polyhydroxyoctanoate; thermal properties