title: LC of high to moderately polar basic drugs in urine with water and detergent, and direct injection.
abstract: BACKGROUND
  Micellar LC was first proposed as a 'green' mode using mobile phases of water and surfactant. However, in most procedures a small amount of organic solvent is required to decrease the retention to convenient values. Results & methodology: Mixed micellar mobile phases prepared with both cationic (sodium dodecyl sulphate) and nonionic surfactant (Brij-35) modulate the retention of high to moderately polar basic drugs to practical times, eliminating the need of organic solvent. While the mobile phase is continuously recycled through the system, the stationary phase performance is maintained after repetitive injection of the samples.
  
  
  CONCLUSIONS
  Through an extensive validation, the approach is shown to be appropriate to determine these drugs in urine samples without previous pretreatment.
authors.affiliation: Departament de Química Analítica, Universitat de València, c/Dr. Moliner 50, 46100 Burjassot, Spain.
authors.forename: María José
authors.initials: MJ
authors.lastname: Ruiz-Ángel
authors.affiliation: Departament de Química Física i Analítica, Universitat Jaume I, Cra. Borriol s/n, 12071 Castelló, Spain.
authors.forename: Samuel
authors.initials: S
authors.lastname: Carda-Broch
authors.affiliation: Departament de Química Analítica, Universitat de València, c/Dr. Moliner 50, 46100 Burjassot, Spain.
authors.forename: María Celia
authors.initials: MC
authors.lastname: García-Álvarez-Coque
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D017022:Flow Injection Analysis / Q000379:methods; D006801:Humans; D057230:Limit of Detection; D008823:Micelles; D004364:Pharmaceutical Preparations / Q000652:urine*; D011092:Polyethylene Glycols / Q000737:chemistry*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D008823:Micelles; D004364:Pharmaceutical Preparations; D013501:Surface-Active Agents; D014867:Water; D012967:Sodium Dodecyl Sulfate; D011092:Polyethylene Glycols; C515901:Brij 35
keywords: Brij-35; basic drugs; direct injection of urine; method validation; micellar LC; sodium dodecyl sulphate; β-adrenoceptor antagonists