title: Analysis of Expression and Functions of Histone Deacetylase 6 (HDAC6).
abstract: Histone deacetylase 6 (HDAC6) is a member of class IIb HDAC family. HDAC6 exists predominantly in the cytoplasm and deacetylates mainly non-histone proteins in the cytoplasm. Via its deacetylase and ubiquitin binding domains, HDAC6 regulates microtubules, cytoskeleton, intracellular trafficking, and cellular responses to stress. HDAC6 plays a central role in physiology and pathobiology in various organs and tissues. Herein we describe the methods for analysis of expression and function of HDAC6 in diverse physiological and pathological contexts.
authors.affiliation: Department of Pathogen Biology, College of Basic Medical Sciences, China Medical University, Shenyang, Liaoning, People's Republic of China.
authors.forename: Miao
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Medicine, Tulane University School of Medicine, New Orleans, LA, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhuang
authors.affiliation: College of Medical Sciences, Washington State University at Spokane, 412 E Spokane Falls Blvd., Spokane, WA, 99202, USA. bin.shan@wsu.edu.
authors.forename: Bin
authors.initials: B
authors.lastname: Shan
mesh_terms: D002460:Cell Line; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D000073864:Histone Deacetylase 6 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D006655:Histone Deacetylases / Q000378:metabolism; D006801:Humans; D047468:Immunoprecipitation; D011485:Protein Binding; D000072417:Protein Domains; D025801:Ubiquitin / Q000378:metabolism
chemical_list: D025801:Ubiquitin; C433811:HDAC6 protein, human; D000073864:Histone Deacetylase 6; D006655:Histone Deacetylases
keywords: Acetylation; Chaperone; Histone deacetylase 6; Stress; Ubiquitin