title: Development and Characterization of Cell-Specific Androgen Receptor Knockout Mice.
abstract: Conditional gene targeting has revolutionized molecular genetic analysis of nuclear receptor proteins, however development and analysis of such conditional knockouts is far from simple, with many caveats and pitfalls waiting to snare the novice or unprepared. In this chapter, we describe our experience of generating and analyzing mouse models with conditional ablation of the androgen receptor (AR) from tissues of the reproductive system and other organs. The guidance, suggestions, and protocols outlined in the chapter provide the key starting point for analyses of conditional-ARKO mice, completing them as described provides an excellent framework for further focussed project-specific analyses, and applies equally well to analysis of reproductive tissues from any mouse model generated through conditional gene targeting.
authors.affiliation: MRC Centre for Reproductive Health, University of Edinburgh, Edinburgh, EH16 4TJ, UK.
authors.forename: Laura
authors.initials: L
authors.lastname: O'Hara
authors.affiliation: MRC Centre for Reproductive Health, University of Edinburgh, Edinburgh, EH16 4TJ, UK. Lee.Smith@ed.ac.uk.
authors.forename: Lee B
authors.initials: LB
authors.lastname: Smith
mesh_terms: D000818:Animals; D018390:Gene Targeting / Q000379:methods*; D017930:Genes, Reporter; D005838:Genotype; D006728:Hormones / Q000378:metabolism; D007124:Immunoenzyme Techniques / Q000379:methods*; D019426:Integrases / Q000378:metabolism; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D008856:Microscopy, Fluorescence / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D011944:Receptors, Androgen / Q000235:genetics / Q000502:physiology*; D013737:Testis / Q000378:metabolism; D019076:Transgenes
chemical_list: C000607144:AR protein, mouse; D006728:Hormones; D011944:Receptors, Androgen; C045073:Cre recombinase; D019426:Integrases
keywords: ARKO; Androgen receptor; Conditional knockout; Cre/lox; Mouse; Transgenic