title: Adipose-Derived Stromal Cells from Lipomas: Isolation, Characterisation and Review of the Literature.
abstract: OBJECTIVE
  The aim of this study was to characterize adipose-derived stromal cells (ADSCs) from patients diagnosed with multiple symmetric lipomatosis (MSL) in order to obtain potentially new insights into the pathophysiology, pathogenesis and treatment of this disease.
  
  
  METHODS
  Cells from the stromal vascular fraction were analysed by the colony-forming efficiency assay and flow cytometry using standard markers. Moreover, the power of adipogenic plasticity was evaluated. Finally, a literature review was performed from 1982 to 2015 using the US National Institutes of Health's PubMed database.
  
  
  RESULTS
  Three European-descent patients diagnosed with either MSL type I or II could be identified for analysis. The resulting mean colony-forming efficiency assay was 14.3 ± 5%. Flow-cytometric analysis of the ADSCs revealed high levels of CD34 (70 ± 9%), CD45 (37 ± 13%) and CD73 (55.8 ± 14%), whereas low levels of CD31 (16.8 ± 14%) and CD105 (5.8 ± 0.7%) were detected. Furthermore, ADSCs showed a strong adipogenic potential, which is in line with the literature review. The stem cell pool in lipoma shows several alterations in biological activities, such as proliferation, apoptosis and stemness.
  
  
  CONCLUSIONS
  ADSCs from lipoma may be interesting in the application of regenerative medicine. We discuss possible molecular treatment options to regulate their activities at the source of the MSL.
authors.affiliation: Department of Plastic, Reconstructive, Aesthetic and Hand Surgery, University Hospital Basel, Basel, Switzerland.
authors.forename: Mathias
authors.initials: M
authors.lastname: Tremp
authors.forename: Nadia
authors.initials: N
authors.lastname: Menzi
authors.forename: Laurent
authors.initials: L
authors.lastname: Tchang
authors.forename: Pietro G
authors.initials: PG
authors.lastname: di Summa
authors.forename: Dirk J
authors.initials: DJ
authors.lastname: Schaefer
authors.forename: Daniel F
authors.initials: DF
authors.lastname: Kalbermatten
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D015703:Antigens, CD / Q000378:metabolism; D017209:Apoptosis; D015415:Biomarkers / Q000378:metabolism; D049109:Cell Proliferation; D002469:Cell Separation; D003114:Colony-Forming Units Assay; D005260:Female; D006801:Humans; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008067:Lipoma / Q000503:physiopathology*; D008069:Lipomatosis, Multiple Symmetrical / Q000503:physiopathology*; D008297:Male; D008875:Middle Aged; D044968:Regenerative Medicine; D013234:Stem Cells / Q000166:cytology; D017154:Stromal Cells / Q000166:cytology; D014481:United States
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; D017493:Leukocyte Common Antigens; C516769:PTPRC protein, human