title: Acinetobacter baumannii and A. pittii clinical isolates lack adherence and cytotoxicity to lung epithelial cells in vitro.
abstract: The molecular and genetic basis of Acinetobacter baumannii and Acinetobacter pittii virulence remains poorly understood, and there is still lack of knowledge in host cell response to these bacteria. In this study, we have used eleven clinical Acinetobacter strains (A. baumannii n = 5; A. pittii n = 6) to unravel bacterial adherence, invasion and cytotoxicity to human lung epithelial cells. Our results showed that adherence to epithelial cells by Acinetobacter strains is scarce and cellular invasion was not truly detected. In addition, all Acinetobacter strains failed to induce any cytotoxic effect on A549 cells.
authors.affiliation: Instituto de Investigación Valdecilla IDIVAL, Santander, Cantabria, Spain; Servicio de Microbiología, Hospital Universitario Marqués de Valdecilla, Santander, Cantabria, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Lázaro-Díez
authors.affiliation: Instituto de Investigación Valdecilla IDIVAL, Santander, Cantabria, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Navascués-Lejarza
authors.affiliation: Instituto de Investigación Valdecilla IDIVAL, Santander, Cantabria, Spain.
authors.forename: Sara
authors.initials: S
authors.lastname: Remuzgo-Martínez
authors.affiliation: Departamento de Biología Molecular, Universidad de Cantabria, Santander, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Navas
authors.affiliation: Departamento de Anatomía y Biología Celular, Universidad de Cantabria, Santander, Spain.
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Icardo
authors.affiliation: Grupo de Investigación en Acuicultura, Universidad de Las Palmas de Gran Canaria, Gran Canaria, Spain.
authors.forename: Felix
authors.initials: F
authors.lastname: Acosta
authors.affiliation: Instituto de Investigación Valdecilla IDIVAL, Santander, Cantabria, Spain; Servicio de Microbiología, Hospital Universitario Marqués de Valdecilla, Santander, Cantabria, Spain; Departamento de Biología Molecular, Universidad de Cantabria, Santander, Spain; Red Española de Investigación en Patología Infecciosa (REIPI), Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Martínez-Martínez
authors.affiliation: Instituto de Investigación Valdecilla IDIVAL, Santander, Cantabria, Spain; Servicio de Microbiología, Hospital Universitario Marqués de Valdecilla, Santander, Cantabria, Spain; Red Española de Investigación en Patología Infecciosa (REIPI), Instituto de Salud Carlos III, Madrid, Spain. Electronic address: jvivas@idival.org.
authors.forename: José
authors.initials: J
authors.lastname: Ramos-Vivas
mesh_terms: D000072283:A549 Cells; D000150:Acinetobacter / Q000302:isolation & purification / Q000502:physiology*; D000151:Acinetobacter Infections / Q000382:microbiology*; D001422:Bacterial Adhesion; D002470:Cell Survival; D004847:Epithelial Cells / Q000382:microbiology* / Q000502:physiology; D006801:Humans; D018410:Pneumonia, Bacterial / Q000382:microbiology*
keywords: Acinetobacter baumannii; Acinetobacter pittii; Adherence; Cytotoxicity; Epithelial cells