title: High-throughput proteomics and the fight against pathogens.
abstract: Pathogens pose a major threat to human and animal welfare. Understanding the interspecies host-pathogen protein-protein interactions could lead to the development of novel strategies to combat infectious diseases through the rapid development of new therapeutics. The first step in understanding the host-pathogen crosstalk is to identify interacting proteins in order to define crucial hot-spots in the host-pathogen interactome, such as the proposed pharmaceutical targets by means of high-throughput proteomic methodologies. In order to obtain holistic insight into the inter- and intra-species bimolecular interactions, apart from the proteomic approach, sophisticated in silico modeling is used to correlate the obtained large data sets with other omics data and clinical outcomes. Since the main focus in this area has been directed towards human medicine, it is time to extrapolate the existing expertise to a new emerging field: the 'systems veterinary medicine'. Therefore, this review addresses high-throughput mass spectrometry-based technology for monitoring protein-protein interactions in vitro and in vivo and discusses pathogen cultivation, model host cells and available bioinformatic tools employed in vaccine development.
authors.affiliation: ERA Chair VetMedZg Project, Internal Diseases Clinic, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10 000 Zagreb, Croatia. horvatic.ani@gmail.com.
authors.forename: Anita
authors.initials: A
authors.lastname: Horvatić
authors.affiliation: ERA Chair VetMedZg Project, Internal Diseases Clinic, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10 000 Zagreb, Croatia. horvatic.ani@gmail.com.
authors.forename: Josipa
authors.initials: J
authors.lastname: Kuleš
authors.affiliation: ERA Chair VetMedZg Project, Internal Diseases Clinic, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10 000 Zagreb, Croatia. horvatic.ani@gmail.com.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Guillemin
authors.affiliation: ERA Chair VetMedZg Project, Internal Diseases Clinic, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10 000 Zagreb, Croatia. horvatic.ani@gmail.com.
authors.forename: Asier
authors.initials: A
authors.lastname: Galan
authors.affiliation: ERA Chair VetMedZg Project, Internal Diseases Clinic, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10 000 Zagreb, Croatia. horvatic.ani@gmail.com.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Mrljak
authors.affiliation: ERA Chair VetMedZg Project, Internal Diseases Clinic, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10 000 Zagreb, Croatia. horvatic.ani@gmail.com and Laboratory of Biomedical Microbiology and Immunology, University of Veterinary Medicine and Pharmacy, Kosice, Slovakia and Institute of Neuroimmunology, Slovakia Academy of Sciences, Bratislava, Slovakia.
authors.forename: Mangesh
authors.initials: M
authors.lastname: Bhide
mesh_terms: D000818:Animals; D003141:Communicable Diseases / Q000209:etiology / Q000378:metabolism; D019295:Computational Biology / Q000379:methods; D057166:High-Throughput Screening Assays; D054884:Host-Pathogen Interactions / Q000235:genetics; D006801:Humans; D025941:Protein Interaction Mapping / Q000379:methods; D040901:Proteomics / Q000379:methods