title: Lipid droplets affect elimination of Porphyromonas gingivalis in HepG2 cells by altering the autophagy-lysosome system.
abstract: Recent studies have shown that infection with Porphyromonas gingivalis, a major periodontal pathogen, hastens the progression of non-alcoholic fatty liver disease (NAFLD). However, the intracellular fate of P. gingivalis in hepatocytes remains unknown. Here, using oleic-acid-induced HepG2 cells as an in vitro model for NAFLD, we found that lipid droplets increased the existence of P. gingivalis in the cells at an early phase of infection. Confocal microscopic analysis revealed that lipid droplets affected the formation of autolysosomes in infected cells. Thus, lipid droplets affect the elimination of P. gingivalis in HepG2 cells by altering the autophagy-lysosome system.
authors.affiliation: Division of Dental Pharmacology, Nagasaki University Graduate School of Biomedical Sciences, Sakamoto 1-7-1, Nagasaki, Nagasaki, 852-8588, Japan.
authors.forename: Yumi
authors.initials: Y
authors.lastname: Zaitsu
authors.affiliation: Division of Dental Pharmacology, Nagasaki University Graduate School of Biomedical Sciences, Sakamoto 1-7-1, Nagasaki, Nagasaki, 852-8588, Japan.
authors.forename: Mayumi
authors.initials: M
authors.lastname: Iwatake
authors.affiliation: Division of Microbiology and Oral Infection, Nagasaki University Graduate School of Biomedical Sciences, Sakamoto 1-7-1, Nagasaki, Nagasaki, 852-8588, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Sato
authors.affiliation: Division of Dental Pharmacology, Nagasaki University Graduate School of Biomedical Sciences, Sakamoto 1-7-1, Nagasaki, Nagasaki, 852-8588, Japan. Electronic address: tsuta@nagasaki-u.ac.jp.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Tsukuba
mesh_terms: D001343:Autophagy; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism / Q000382:microbiology* / Q000502:physiology*; D006801:Humans; D066292:Lipid Droplets / Q000378:metabolism*; D008247:Lysosomes / Q000378:metabolism*; D018613:Microscopy, Confocal; D016966:Porphyromonas gingivalis / Q000502:physiology*
keywords: Autophagy; HepG2 cells; Lysosome; Non-alcoholic fatty liver disease; Porphyromonas gingivalis