title: Developmental validation of STRmix™, expert software for the interpretation of forensic DNA profiles.
abstract: In 2015 the Scientific Working Group on DNA Analysis Methods published the SWGDAM Guidelines for the Validation of Probabilistic Genotyping Systems [1]. STRmix™ is probabilistic genotyping software that employs a continuous model of DNA profile interpretation. This paper describes the developmental validation activities of STRmix™ following the SWGDAM guidelines. It addresses the underlying scientific principles, and the performance of the models with respect to sensitivity, specificity and precision and results of interpretation of casework type samples. This work demonstrates that STRmix™ is suitable for its intended use for the interpretation of single source and mixed DNA profiles.
authors.affiliation: ESR, Private Bag 92021, Auckland 1142, New Zealand. Electronic address: jodashanne@gmail.com.
authors.forename: Jo-Anne
authors.initials: JA
authors.lastname: Bright
authors.affiliation: Forensic Science South Australia, 21 Divett Place, Adelaide, SA 5000, Australia; School of Biological Sciences, Flinders University, GPO Box 2100, Adelaide, SA 5001, Australia.
authors.forename: Duncan
authors.initials: D
authors.lastname: Taylor
authors.affiliation: ESR, Private Bag 92021, Auckland 1142, New Zealand.
authors.forename: Catherine
authors.initials: C
authors.lastname: McGovern
authors.affiliation: ESR, Private Bag 92021, Auckland 1142, New Zealand.
authors.forename: Stuart
authors.initials: S
authors.lastname: Cooper
authors.affiliation: ESR, Private Bag 92021, Auckland 1142, New Zealand.
authors.forename: Laura
authors.initials: L
authors.lastname: Russell
authors.affiliation: Forensic Science South Australia, 21 Divett Place, Adelaide, SA 5000, Australia.
authors.forename: Damien
authors.initials: D
authors.lastname: Abarno
authors.affiliation: ESR, Private Bag 92021, Auckland 1142, New Zealand.
authors.forename: John
authors.initials: J
authors.lastname: Buckleton
mesh_terms: D016172:DNA Fingerprinting; D005838:Genotype; D006801:Humans; D016013:Likelihood Functions; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D012984:Software
keywords: Continuous method; DNA mixtures; Probabilistic genotyping; STRmix; Validation