title: Complete sequence of a double-stranded RNA from the phytopathogenic fungus Erysiphe cichoracearum that might represent a novel endornavirus.
abstract: A double-stranded RNA (dsRNA) HBJZ1506 recovered from the phytopathogenic fungus Erysiphe cichoracearum infecting Calendula officinalis in Jingzhou, Hubei Province, China, was sequenced. HBJZ1506 comprises 11,908 nucleotides (nt) and contains a 11,859-nt-long open reading frame (ORF) coding for a polypeptide that is 61 % identical to that of a putative endornavirus named grapevine endophyte endornavirus (GeEV). The putative polyprotein has an RNA-dependent RNA polymerase (RdRp) domain and an RNA helicase domain, which show homology to and have an arrangement that is similar to that of their counterparts in approved or putative endornaviruses. In a phylogenetic tree constructed using amino acid sequences of the RdRp region of HBJZ1506 and selected endornaviruses, HBJZ1506 clustered with endornaviruses and formed a well-supported monophyletic branch with GeEV. These results suggest that HBJZ1506 might represent a novel endornavirus, for which the name Erysiphe cichoracearum endornavirus (EcEV) is proposed.
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Plant Protection College, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.
authors.forename: Zhenguo
authors.initials: Z
authors.lastname: Du
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Plant Protection College, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.
authors.forename: Wenzhong
authors.initials: W
authors.lastname: Lin
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Plant Protection College, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Qiu
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Plant Protection College, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Engineering Research Centre of Ecology and Agricultural Use of Wetland, Ministry of Education, Yangtze University, Jingzhou, 434025, Hubei, China.
authors.forename: Lingfang
authors.initials: L
authors.lastname: Guo
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Plant Protection College, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.
authors.forename: Kangcheng
authors.initials: K
authors.lastname: Wu
authors.affiliation: Engineering Research Centre of Ecology and Agricultural Use of Wetland, Ministry of Education, Yangtze University, Jingzhou, 434025, Hubei, China. yangtze2008@126.com.
authors.forename: Songbai
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Plant Protection College, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China. wuzujian@126.com.
authors.forename: Zujian
authors.initials: Z
authors.lastname: Wu
mesh_terms: D001203:Ascomycota / Q000821:virology*; D001483:Base Sequence; D002681:China; D016679:Genome, Viral; D023281:Genomics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D012328:RNA Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012330:RNA, Double-Stranded / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012330:RNA, Double-Stranded; D012367:RNA, Viral; D014764:Viral Proteins