title: Protocols for Developing Novel Chikungunya Virus DNA Vaccines.
abstract: To date, there have been several million infections by the Chikungunya virus (CHIKV), a mosquito-transmitted emerging pathogen that is considered to be taxonomically an Old World RNA virus. Although original CHIKV outbreaks were restricted to India, East Asian countries, Northern Italy, and France, a recent sharp rise had been identified in 41 countries or territories in the Caribbean, Central America, South America, and North America. A total of 1,012,347 suspected and 22,579 laboratory-confirmed CHIKV cases have been reported from these areas, which signals an increasing risk to the US mainland. Unlike past epidemics that were usually associated with Ae. aegypti transmission, the Caribbean outbreak was associated with Ae. albopictus transmission as the principal mosquito vector. In addition, the substantial increase in the number of deaths during this epidemic, as well as incidence of neurologic disease, suggests that CHIKV may have become more virulent. Currently, there are no licensed vaccines or therapeutics available for CHIKV or its associated disease pathologies. Therefore, development of new vaccines and therapies that could confer immunity and/or treat clinical symptoms of CHIKV is greatly desired. This chapter describes the use of entirely cutting edge technologies/methodologies developed by our group for the development and evaluation of novel DNA vaccines against CHIKV.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, 19104, USA.
authors.forename: Christopher
authors.initials: C
authors.lastname: Chung
authors.affiliation: Department of Molecular Medicine, University of South Florida Morsani College of Medicine, Tampa, FL, 33612, USA.
authors.forename: Kenneth E
authors.initials: KE
authors.lastname: Ugen
authors.affiliation: Inovio Pharmaceuticals, Plymouth Meeting, PA, 19462, USA.
authors.forename: Niranjan Y
authors.initials: NY
authors.lastname: Sardesai
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, 19104, USA.
authors.forename: David B
authors.initials: DB
authors.lastname: Weiner
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, 19104, USA. muthuman@mail.med.upenn.edu.
authors.forename: Kar
authors.initials: K
authors.lastname: Muthumani
mesh_terms: D000818:Animals; D002646:Chikungunya virus / Q000235:genetics* / Q000276:immunology; D004195:Disease Models, Animal; D057809:HEK293 Cells; D006801:Humans; D007114:Immunization; D051379:Mice; D009500:Neutralization Tests; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000235:genetics / Q000276:immunology*
chemical_list: D019444:Vaccines, DNA
keywords: Cellular immunity; Chikungunya virus; DNA vaccine; Envelope protein; Humoral responses; Neutralization assay