title: A double responsive smart upconversion fluorescence sensing material for glycoprotein.
abstract: A novel strategy was developed to prepare double responsive smart upconversion fluorescence material for highly specific enrichment and sensing of glycoprotein. The novel double responsive smart sensing material was synthesized by choosing Horse radish peroxidase (HRP) as modal protein, the grapheme oxide (GO) as support material, upconversion nanoparticles (UCNPs) as fluorescence signal reporter, N-isopropyl acrylamide (NIPAAM) and 4-vinylphenylboronic acid (VPBA) as functional monomers. The structure and component of smart sensing material was investigated by transmission electron microscopy (TEM), Scanning electron microscopy (SEM), X-ray photoelectron spectroscopic (XPS) and Fourier transform infrared (FTIR), respectively. These results illustrated the smart sensing material was prepared successfully. The recognition characterizations of smart sensing material were evaluated, and results showed that the fluorescence intensity of smart sensing material was reduced gradually, as the concentration of protein increased, and the smart sensing material showed selective recognition for HRP among other proteins. Furthermore, the recognition ability of the smart sensing material for glycoprotein was regulated by controlling the pH value and temperature. Therefore, this strategy opens up new way to construct smart material for detection of glycoprotein.
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Guo
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China. Electronic address: yhdql@tust.edu.cn.
authors.forename: Qiliang
authors.initials: Q
authors.lastname: Deng
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China.
authors.forename: Guozhen
authors.initials: G
authors.lastname: Fang
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China.
authors.forename: Yaguang
authors.initials: Y
authors.lastname: Yun
authors.affiliation: Institute of Food Science and Technology, Yunnan Agricultural University, Yunnan 650201, China.
authors.forename: Yongjin
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China. Electronic address: s.wang@tust.edu.cn.
authors.forename: Shuo
authors.initials: S
authors.lastname: Wang
mesh_terms: D000178:Acrylamides / Q000737:chemistry; D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D001897:Boronic Acids / Q000737:chemistry; D002417:Cattle; D005456:Fluorescent Dyes / Q000737:chemistry*; D006023:Glycoproteins / Q000032:analysis*; D006108:Graphite / Q000737:chemistry*; D008958:Models, Molecular; D054802:Molecular Imprinting; D053758:Nanoparticles / Q000737:chemistry*; D010047:Ovalbumin / Q000032:analysis; D010087:Oxides / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000032:analysis; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D000178:Acrylamides; D001897:Boronic Acids; D005456:Fluorescent Dyes; D006023:Glycoproteins; D010087:Oxides; D012710:Serum Albumin, Bovine; D006108:Graphite; D010047:Ovalbumin; C067295:N-isopropylacrylamide
keywords: Glycoprotein; Graphene oxide; Molecular imprinting; Smart material; Upconversion nanoparticles