title: Semi-automated microplate monitoring of protein polymerization and aggregation.
abstract: Static light scattering (SLS) is a commonly used technique for monitoring dynamics of high molecular weight protein complexes such as protein oligomers or aggregates. However, traditional methods are limited to testing a single condition and typically require large amounts of protein and specialized equipment. We show that a standard microplate reader can be used to characterize the molecular dynamics of different types of protein complexes, with the multiple advantages of microscale experimental volumes, semi-automated protocols and highly parallel processing.
authors.affiliation: Department of Microbiology and Molecular Genetics, University of Texas McGovern Medical School at Houston, Houston, TX, 77030, USA; University of Texas Graduate School of Biomedical Sciences, Houston, TX, 77030, USA.
authors.forename: Veronica M
authors.initials: VM
authors.lastname: Garcia
authors.affiliation: Department of Microbiology and Molecular Genetics, University of Texas McGovern Medical School at Houston, Houston, TX, 77030, USA; University of Texas Graduate School of Biomedical Sciences, Houston, TX, 77030, USA.
authors.forename: Veronica W
authors.initials: VW
authors.lastname: Rowlett
authors.affiliation: Department of Microbiology and Molecular Genetics, University of Texas McGovern Medical School at Houston, Houston, TX, 77030, USA. Electronic address: William.margolin@uth.tmc.edu.
authors.forename: William
authors.initials: W
authors.lastname: Margolin
authors.affiliation: Department of Microbiology and Molecular Genetics, University of Texas McGovern Medical School at Houston, Houston, TX, 77030, USA. Electronic address: kevin.a.morano@uth.tmc.edu.
authors.forename: Kevin A
authors.initials: KA
authors.lastname: Morano
mesh_terms: D057205:Automation, Laboratory; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation / Q000379:methods*; D056004:Molecular Dynamics Simulation; D058105:Polymerization; D066329:Protein Aggregates; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D066329:Protein Aggregates; D011506:Proteins
keywords: Chaperone; Cytoskeleton; Protein aggregation; Protein assembly; Protein dynamics