title: Selective adsorption of protein by a high-efficiency Cu(2+) -cooperated magnetic imprinted nanomaterial.
abstract: We report a core-shell magnetic molecularly imprinted polymer with high affinity through a facile sol-gel method for the selective adsorption of bovine hemoglobin from real bovine blood. Copper ions grafted on the surface of the matrix could immobilize template protein through chelation, which greatly enhances the orderliness of imprinted cavities and affinity of polymers. The obtained products exhibit a desired level of magnetic susceptibility, resulting in the highly efficient adsorption process. The results of adsorption experiments show that the saturation adsorption capacity of imprinted products could reach 116.3 mg/g within 30 min. Meanwhile, the specific binding experiment demonstrates the high selectivity of polymers for bovine hemoglobin. Furthermore, satisfactory reusability is demonstrated by ten adsorption-desorption cycles with no obvious deterioration in binding capacity. Electrophoretic analysis suggests the polymer could be used successfully in separation and enrichment of bovine hemoglobin from the bovine blood sample, which exhibits potential application in pretreatment of proteomics.
authors.affiliation: Institute of Analytical Science, School of Science, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Shi
authors.affiliation: Institute of Analytical Science, School of Science, Xi'an Jiaotong University, Xi'an, China.|School of Pharmacy, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Yuhai
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Institute of Analytical Science, School of Science, Xi'an Jiaotong University, Xi'an, China.|School of Pharmacy, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Hao
authors.affiliation: Institute of Analytical Science, School of Science, Xi'an Jiaotong University, Xi'an, China.|School of Pharmacy, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Gaiyan
authors.initials: G
authors.lastname: He
authors.affiliation: Institute of Analytical Science, School of Science, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Ruixia
authors.initials: R
authors.lastname: Gao
authors.affiliation: Department of Urology, The Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an, China.
authors.forename: Xiaoshuang
authors.initials: X
authors.lastname: Tang
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D002614:Chelating Agents / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry / Q000302:isolation & purification*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D054802:Molecular Imprinting; D011108:Polymers / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D002614:Chelating Agents; D006454:Hemoglobins; D058185:Magnetite Nanoparticles; D011108:Polymers; D003300:Copper
keywords: Metal coordination; Protein; Surface imprinting