title: A gene and protein expression study on four porcine genes related to intramuscular fat deposition.
abstract: Intramuscular fat (IMF) content has a prominent role in meat quality, affecting sensory attributes such as flavour and texture. In the present research, we studied in samples of porcine Semimembranosus muscle four genes related to lipid metabolism and whose gene expressions have been associated to IMF deposition: FASN, SCD, LIPE and LPL. We analysed both mRNA and protein expressions in two groups of Italian Large White pigs divergent for Semimembranosus IMF deposition, with the aim of comparing the levels of four genes and enzymes between the two groups and identifying possible coexpression links. The obtained results suggest a prominent role of LIPE enzyme in IMF hydrolysis, as the samples with low IMF deposition show a significantly higher amount of this lipase. Finally, a poorly known correlation was found between LIPE and FASN enzymes only in female individuals. These results provide new information for the understanding of IMF deposition.
authors.affiliation: Department of Agricultural and Food Sciences (DISTAL), University of Bologna, Viale Fanin 46, 40127 Bologna, Italy. Electronic address: martina.zappaterra2@unibo.it.
authors.forename: Martina
authors.initials: M
authors.lastname: Zappaterra
authors.affiliation: Center for Applied Biomedical Research (C.R.B.A.), S. Orsola-Malpighi University Hospital, University of Bologna, Via Massarenti 9, 40138 Bologna, Italy; Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Via Massarenti 9, 40138 Bologna, Italy. Electronic address: marzia.deserti@gmail.com.
authors.forename: Marzia
authors.initials: M
authors.lastname: Deserti
authors.affiliation: Center for Applied Biomedical Research and Endocrinology Unit, Department of Medical and Surgical Sciences, University of Bologna, Via Massarenti 9, 40138 Bologna, Italy. Electronic address: mazza.roberta@gmail.com.
authors.forename: Roberta
authors.initials: R
authors.lastname: Mazza
authors.affiliation: Department of Agricultural and Food Sciences (DISTAL), University of Bologna, Viale Fanin 46, 40127 Bologna, Italy. Electronic address: silvia_braglia@yahoo.it.
authors.forename: Silvia
authors.initials: S
authors.lastname: Braglia
authors.affiliation: Department of Agricultural and Food Sciences (DISTAL), University of Bologna, Viale Fanin 46, 40127 Bologna, Italy. Electronic address: paolo.zambonelli@unibo.it.
authors.forename: Paolo
authors.initials: P
authors.lastname: Zambonelli
authors.affiliation: Department of Agricultural and Food Sciences (DISTAL), University of Bologna, Viale Fanin 46, 40127 Bologna, Italy. Electronic address: roberta.davoli@unibo.it.
authors.forename: Roberta
authors.initials: R
authors.lastname: Davoli
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D064429:Fatty Acid Synthases / Q000235:genetics / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D008049:Lipase / Q000235:genetics / Q000378:metabolism; D050356:Lipid Metabolism; D008071:Lipoprotein Lipase / Q000235:genetics / Q000378:metabolism; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D013230:Stearoyl-CoA Desaturase / Q000235:genetics / Q000378:metabolism; D013552:Swine / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D013230:Stearoyl-CoA Desaturase; D064429:Fatty Acid Synthases; D008049:Lipase; D008071:Lipoprotein Lipase
keywords: Adipogenesis; FASN; Fat genes; Gene expression; LIPE; LPL; Lipid metabolism; Meat quality; Muscle fat; Pig; Protein; SCD