title: MicroRNA-223 Promotes Type I Interferon Production in Antiviral Innate Immunity by Targeting Forkhead Box Protein O3 (FOXO3).
abstract: Effective recognition of viral infection and subsequent triggering of antiviral innate immune responses are essential for the host antiviral defense, which is tightly regulated by multiple regulators, including microRNAs. Previous reports have shown that some microRNAs are induced during virus infection and participate in the regulation of the innate antiviral response. However, whether the type I IFN response is regulated by miR-223 is still unknown. Here, we reported that vesicular stomatitis virus (VSV) infection induced significant up-regulation of miR-223 in murine macrophages. We observed that miR-223 overexpression up-regulated type I IFN expression levels in VSV-infected macrophages. We also demonstrated that miR-223 directly targets FOXO3 to regulate the type I IFN production. Furthermore, type I IFN, which is triggered by VSV infection, is responsible for the up-regulation of miR-223, thus forming a positive regulatory loop for type I IFN production. Our results uncovered a novel mechanism of miR-223-mediated regulation of type I IFN production in the antiviral innate immunity for the first time.
authors.affiliation: From the Institute of Immunology, Zhejiang University School of Medicine, Hangzhou 310058, China.
authors.forename: Luoquan
authors.initials: L
authors.lastname: Chen
authors.affiliation: From the Institute of Immunology, Zhejiang University School of Medicine, Hangzhou 310058, China.
authors.forename: Yinjing
authors.initials: Y
authors.lastname: Song
authors.affiliation: From the Institute of Immunology, Zhejiang University School of Medicine, Hangzhou 310058, China.
authors.forename: Li
authors.initials: L
authors.lastname: He
authors.affiliation: From the Institute of Immunology, Zhejiang University School of Medicine, Hangzhou 310058, China.
authors.forename: Xiaopeng
authors.initials: X
authors.lastname: Wan
authors.affiliation: From the Institute of Immunology, Zhejiang University School of Medicine, Hangzhou 310058, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Lai
authors.affiliation: From the Institute of Immunology, Zhejiang University School of Medicine, Hangzhou 310058, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Dai
authors.affiliation: From the Institute of Immunology, Zhejiang University School of Medicine, Hangzhou 310058, China liuyang0620@zju.edu.cn.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: From the Institute of Immunology, Zhejiang University School of Medicine, Hangzhou 310058, China wqq@zju.edu.cn.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000818:Animals; D002460:Cell Line; D000071316:Forkhead Box Protein O3 / Q000276:immunology*; D057809:HEK293 Cells; D006801:Humans; D007113:Immunity, Innate; D007370:Interferon Type I / Q000276:immunology*; D008264:Macrophages / Q000276:immunology / Q000378:metabolism / Q000821:virology*; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000235:genetics / Q000276:immunology*; D054243:Vesicular Stomatitis / Q000235:genetics / Q000276:immunology*; D018116:Vesiculovirus / Q000276:immunology* / Q000502:physiology; D014779:Virus Replication
chemical_list: D000071316:Forkhead Box Protein O3; C474033:FoxO3 protein, mouse; D007370:Interferon Type I; C544877:MIRN223 microRNA, mouse; D035683:MicroRNAs
keywords: FOXO3; IRF7; antiviral agent; innate immunity; interferon; interferon regulatory factor (IRF); microRNA (miRNA); microRNA-223; type I interferon