title: Rapid screening for drugs of abuse in biological fluids by ultra high performance liquid chromatography/Orbitrap mass spectrometry.
abstract: We present a UPLC(®)-High Resolution Mass Spectrometric method to simultaneously screen for nineteen benzodiazepines, twelve opiates, cocaine and three metabolites, and three "Z-drug" hypnotic sedatives in both blood and urine specimens. Sample processing consists of a high-speed, high-temperature enzymatic hydrolysis for urine samples followed by a rapid supported liquid extraction (SLE). The combination of ultra-high resolution chromatography with high resolution mass spectrometry allows all 38 analytes to be uniquely detected with a ten minute analytical run. Limits of detection for all target analytes are 3ng/mL or better, with only 0.3mL of specimen used for analysis. The combination of low sample volume with fast processing and analysis makes this method a suitable replacement for immunoassay screening of the targeted drug classes, while providing far superior specificity and better limits of detection than can routinely be obtained by immunoassay.
authors.affiliation: Federal Bureau of Investigation Laboratory, Quantico, VA 22135, United States. Electronic address: Eshwar.Jagerdeo@ic.fbi.gov.
authors.forename: Eshwar
authors.initials: E
authors.lastname: Jagerdeo
authors.affiliation: Federal Bureau of Investigation Laboratory, Quantico, VA 22135, United States.
authors.forename: Jason E
authors.initials: JE
authors.lastname: Schaff
mesh_terms: D001569:Benzodiazepines / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D003042:Cocaine / Q000097:blood / Q000652:urine; D004867:Equipment Design; D006801:Humans; D006993:Hypnotics and Sedatives / Q000097:blood / Q000652:urine; D057230:Limit of Detection; D053610:Opiate Alkaloids / Q000097:blood / Q000652:urine; D021241:Spectrometry, Mass, Electrospray Ionization / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D015813:Substance Abuse Detection / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors
chemical_list: D006993:Hypnotics and Sedatives; D053610:Opiate Alkaloids; D001569:Benzodiazepines; D003042:Cocaine
keywords: Drugs of abuse; LC–MS; Orbitrap; Screening procedure; UPLC(®)