title: Cloning and characterization of giant panda (Ailuropoda melanoleuca) IL-18 binding protein.
abstract: The giant panda (Ailuropoda melanoleuca) is an endangered species. Interleukin-18 (IL-18) plays an important role in the innate and adaptive immune responses by inducing IFN-γ. IL-18 has been implicated in the pathogenesis of various diseases. IL-18 binding protein (IL-18BP) is an intrinsic inhibitor of IL-18 that possesses higher affinity to IL-18. In this study, we cloned and characterized IL-18BP in giant panda (AmIL-18BP) from the spleen. The amino acid sequence of giant panda IL-18BP ORF shared about 65% identities with other species. To evaluate the effects of AmIL-18BP on the immune responses, we expressed the recombinant AmIL-18BP in Escherichia coli BL21 (DE3).The fusing protein PET-AmIL-18BP was purified by nickel affinity column chromatography. The biological function of purified PET-AmIL-18BP was determined on mice splenocyte by qRT-PCR. The results showed that AmIL-18BP was functional and could significantly reduce IFN-γ production in murine splenocytes. These results will facilitate the study of protecting giant panda on etiology and immunology.
authors.affiliation: College of Life Sciences, Sichuan University, Key Laboratory for Bio-Resources and Eco-Environment of Ministry of Education, Sichuan Key Laboratory of Molecular Biology and Biotechnology, Chengdu 610064, PR China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Chengdu Zoo, Chengdu 610081, PR China.
authors.forename: Jiabo
authors.initials: J
authors.lastname: Deng
authors.affiliation: Chengdu Zoo, Chengdu 610081, PR China.
authors.forename: Lili
authors.initials: L
authors.lastname: Niu
authors.affiliation: Chengdu Zoo, Chengdu 610081, PR China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Chengdu Zoo, Chengdu 610081, PR China.
authors.forename: Jianqiu
authors.initials: J
authors.lastname: Yu
authors.affiliation: College of Life Sciences, Sichuan University, Key Laboratory for Bio-Resources and Eco-Environment of Ministry of Education, Sichuan Key Laboratory of Molecular Biology and Biotechnology, Chengdu 610064, PR China.
authors.forename: Huanhuan
authors.initials: H
authors.lastname: Shao
authors.affiliation: College of Life Sciences, Sichuan University, Key Laboratory for Bio-Resources and Eco-Environment of Ministry of Education, Sichuan Key Laboratory of Molecular Biology and Biotechnology, Chengdu 610064, PR China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Cao
authors.affiliation: College of Life Sciences, Sichuan University, Key Laboratory for Bio-Resources and Eco-Environment of Ministry of Education, Sichuan Key Laboratory of Molecular Biology and Biotechnology, Chengdu 610064, PR China.
authors.forename: Yizheng
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Sichuan University, Key Laboratory for Bio-Resources and Eco-Environment of Ministry of Education, Sichuan Key Laboratory of Molecular Biology and Biotechnology, Chengdu 610064, PR China. Electronic address: txmyyf@scu.edu.cn.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Tan
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism; D051379:Mice; D020539:Sequence Analysis, Protein / Q000662:veterinary; D013154:Spleen / Q000276:immunology; D001503:Ursidae / Q000235:genetics* / Q000276:immunology
chemical_list: D018076:DNA, Complementary; C000611649:IFNG protein, mouse; D036341:Intercellular Signaling Peptides and Proteins; C118370:interleukin-18 binding protein; D007371:Interferon-gamma
keywords: Bioactivity analysis; Giant panda; IL-18 binding protein; Interleukin-18