title: Molecular cloning and functional analysis of a 10-epi-junenol synthase from Inula hupehensis.
abstract: Junenol based-eudesmanolides have been detected in many compositae plant species and were reported to exhibit various pharmacological activities. So far, the gene encoding junenol synthase has never been isolated. Here we report the molecular cloning and functional analysis of a 10-epi-junenol synthase from Inula hupehensis (designated IhsTPS1). IhsTPS1 converts the substrate farnesyl diphosphate into multiple sesquiterpenes with the product 10-epi-junenol being predominant. The transcript levels of IhsTPS1 correlate well with the accumulation pattern of 10-epi-junenol in I. hupehensis organs, supporting its biochemical roles in vivo.
authors.affiliation: CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430071, China; Graduate University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Jun-Bo
authors.initials: JB
authors.lastname: Gou
authors.affiliation: College of Life Sciences, Hebei University, Baoding 071002, China.
authors.forename: Zhen-Qiu
authors.initials: ZQ
authors.lastname: Li
authors.affiliation: CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430071, China.
authors.forename: Chang-Fu
authors.initials: CF
authors.lastname: Li
authors.affiliation: CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430071, China.
authors.forename: Fang-Fang
authors.initials: FF
authors.lastname: Chen
authors.affiliation: CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430071, China.
authors.forename: Shi-You
authors.initials: SY
authors.lastname: Lv
authors.affiliation: CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430071, China. Electronic address: zhangys@wbgcas.cn.
authors.forename: Yan-Sheng
authors.initials: YS
authors.lastname: Zhang
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000235:genetics* / Q000378:metabolism; D053898:Biosynthetic Pathways / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D008401:Gas Chromatography-Mass Spectrometry; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D038143:Inula / Q000201:enzymology* / Q000235:genetics*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011106:Polyisoprenyl Phosphates / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012717:Sesquiterpenes / Q000737:chemistry / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D010940:Plant Proteins; D011106:Polyisoprenyl Phosphates; D012333:RNA, Messenger; D012717:Sesquiterpenes; C476916:eudesmanolide; C004808:farnesyl pyrophosphate; D019883:Alkyl and Aryl Transferases; C108877:terpene synthase
keywords: 10-Epi-junenol synthase; Eudesmanolide; Inula hupehensis