title: High intensity interval training in the heat enhances exercise-induced lipid peroxidation, but prevents protein oxidation in physically active men.
abstract: Aim. The purpose of this study was to determine the response of circulating markers of lipid and protein oxidation following an incremental test to exhaustion before and after 4 weeks of high-intensity interval training performed in the heat. Methods. To address this question, 16 physically active men (age = 23 ± 2 years; body mass = 73 ± 12 kg; height = 173 ± 6 cm; % body fat = 12.5 ± 6 %; body mass index = 24 ± 4 kg/m(2)) were allocated into 2 groups: control group (n = 8) performing high-intensity interval training at 22°C, 55% relative humidity and heat group (n = 8) training under 35°C, 55% relative humidity. Both groups performed high-intensity interval training 3 times per week for 4 consecutive weeks, accumulating a total of 12 training sessions. Before and after the completion of 4 weeks of high-intensity interval training, participants performed an incremental cycling test until exhaustion under temperate environment (22°C, 55% relative humidity) where blood samples were collected after the test for determination of exercise-induced changes in oxidative damage biomarkers (thiobarbituric acid reactive species and protein carbonyls). Results. When high-intensity interval training was performed under control conditions, there was an increase in protein carbonyls (p < 0.05) following the incremental test to exhaustion with no changes in thiobarbituric acid reactive species. Conversely, high-intensity interval training performed in high environmental temperature enhanced the incremental exercise-induced increases in thiobarbituric acid reactive species (p < 0.05) with no changes in protein carbonyls. Conclusion. In conclusion, 4 weeks of high-intensity interval training performed in the heat enhances exercise-induced lipid peroxidation, but prevents protein oxidation following a maximal incremental exercise in healthy active men.
authors.affiliation: College of Physical Education, Federal University of Vale do São Francisco , Petrolina/PE, Brazil.
authors.forename: Ana Angélica
authors.initials: AA
authors.lastname: Souza-Silva
authors.affiliation: College of Physical Education, Federal University of Vale do São Francisco , Petrolina/PE, Brazil.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Moreira
authors.affiliation: College of Physical Education, Federal University of Vale do São Francisco , Petrolina/PE, Brazil.
authors.forename: Denise
authors.initials: D
authors.lastname: de Melo-Marins
authors.affiliation: Department of Physiology, Federal University of Rio Grande do Sul , Porto Alegre/RS, Brazil.
authors.forename: Cinthia M
authors.initials: CM
authors.lastname: Schöler
authors.affiliation: Department of Physiology, Federal University of Rio Grande do Sul , Porto Alegre/RS, Brazil.
authors.forename: Paulo Ivo Homem
authors.initials: PI
authors.lastname: de Bittencourt
authors.affiliation: College of Physical Education, Federal University of Vale do São Francisco , Petrolina/PE, Brazil.
authors.forename: Orlando
authors.initials: O
authors.lastname: Laitano
keywords: TBARS; exercise; hyperthermia; oxidative stress; protein carbonyls