title: Development of soybeans with low P34 allergen protein concentration for reduced allergenicity of soy foods.
abstract: BACKGROUND
  In soybean, at least 16 seed proteins have been identified as causing allergenic reactions in sensitive individuals. As a soybean genebank accession low in the immunodominant protein P34 (Gly m Bd 30K) has recently been found, introgression of the low-P34 trait into adapted soybean germplasm has been attempted in order to improve the safety of food products containing soybean protein. Therefore, marker-assisted selection and proteomics were applied to identify and characterize low-P34 soybeans.
  
  
  RESULTS
  In low-P34 lines selected from a cross-population, concentrations of the P34 protein as identified with a polyclonal antibody were reduced by 50-70% as compared to P34-containing controls. Using 2D electrophoresis and immunoblotting, the reduction of P34 protein was verified in low-P34 lines. This result was confirmed by liquid chromatographic-tandem mass spectrometric analysis, which revealed either a reduction or complete absence of the authentic P34 protein as suggested from presence or absence of a unique peptide useful for discriminating between conventional and low-P34 lines.
  
  
  CONCLUSIONS
  Marker-assisted selection proved useful for identifying low-P34 soybean lines for the development of hypoallergenic soy foods. The status of the P34 protein in low-P34 lines needs further characterization. In addition, the food safety relevance of low-P34 soybeans should be tested in clinical studies. © 2016 Society of Chemical Industry.
authors.affiliation: Department of Crop Sciences, Division of Plant Breeding, University of Natural Resources and Life Sciences Vienna (BOKU), 3430, Tulln an der Donau, Austria.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Watanabe
authors.affiliation: National Agricultural Research and Innovation Centre, Food Science Research Institute (NARIC-FSRI), 1022, Budapest, Hungary.
authors.forename: Nóra
authors.initials: N
authors.lastname: Adányi
authors.affiliation: National Agricultural Research and Innovation Centre, Food Science Research Institute (NARIC-FSRI), 1022, Budapest, Hungary.
authors.forename: Krisztina
authors.initials: K
authors.lastname: Takács
authors.affiliation: National Agricultural Research and Innovation Centre, Food Science Research Institute (NARIC-FSRI), 1022, Budapest, Hungary.
authors.forename: Anita
authors.initials: A
authors.lastname: Maczó
authors.affiliation: National Agricultural Research and Innovation Centre, Food Science Research Institute (NARIC-FSRI), 1022, Budapest, Hungary.
authors.forename: András
authors.initials: A
authors.lastname: Nagy
authors.affiliation: National Agricultural Research and Innovation Centre, Food Science Research Institute (NARIC-FSRI), 1022, Budapest, Hungary.
authors.forename: Éva
authors.initials: É
authors.lastname: Gelencsér
authors.affiliation: Department of Crop Sciences, Division of Plant Breeding, University of Natural Resources and Life Sciences Vienna (BOKU), 3430, Tulln an der Donau, Austria.
authors.forename: Martin
authors.initials: M
authors.lastname: Pachner
authors.affiliation: Department IFA-Tulln, Center for Analytical Chemistry, University of Natural Resources and Life Sciences Vienna (BOKU), 3430, Tulln an der Donau, Austria.
authors.forename: Kathrin
authors.initials: K
authors.lastname: Lauter
authors.affiliation: Department IFA-Tulln, Center for Analytical Chemistry, University of Natural Resources and Life Sciences Vienna (BOKU), 3430, Tulln an der Donau, Austria.
authors.forename: Sabine
authors.initials: S
authors.lastname: Baumgartner
authors.affiliation: Department of Crop Sciences, Division of Plant Breeding, University of Natural Resources and Life Sciences Vienna (BOKU), 3430, Tulln an der Donau, Austria.
authors.forename: Johann
authors.initials: J
authors.lastname: Vollmann
mesh_terms: D052179:Antigens, Plant / Q000009:adverse effects* / Q000235:genetics / Q000378:metabolism; D003433:Crosses, Genetic; D015536:Down-Regulation; D004797:Enzyme-Linked Immunosorbent Assay; D005103:Expert Systems; D005512:Food Hypersensitivity / Q000178:diet therapy / Q000517:prevention & control; D059022:Food Safety; D055951:Functional Food / Q000009:adverse effects / Q000032:analysis; D018506:Gene Expression Regulation, Plant; D008040:Genetic Linkage; D005819:Genetic Markers; D006801:Humans; D018895:Microsatellite Repeats; D000069600:Plant Breeding; D040901:Proteomics / Q000379:methods; D012639:Seeds / Q000009:adverse effects / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D012641:Selection, Genetic; D045730:Soy Foods / Q000009:adverse effects / Q000032:analysis*; D030262:Soybean Proteins / Q000009:adverse effects* / Q000235:genetics / Q000378:metabolism; D013025:Glycine max / Q000009:adverse effects / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism
chemical_list: D052179:Antigens, Plant; D005819:Genetic Markers; D030262:Soybean Proteins; C075135:P34 protein, Glycine max
keywords: Gly m Bd 30 K protein; food allergy; marker-assisted selection; proteomics; soybean