title: A pyrazolo[1,5-a]pyridine-fused pyrimidine based novel fluorophore and its bioapplication to probing lipid droplets.
abstract: A new fluorescent core skeleton containing pyrazolo[1,5-a]pyridine-fused pyrimidine, called fluoremidine (FD), was discovered. FD analogues were prepared via a one-pot silver-catalyzed cascade cyclization. An N,N-dimethylamino group at the R(1)- and R(2)-positions plays important roles in controlling fluorescence brightness and emission wavelength. An N-acetyl group at the R(3) position contributes to red shifting of the emission wavelength. FD shows excellent solvatochromism with turn-on fluorescence in the lipophilic environment, which was utilized to design a fluorescent probe, FD13, for visualizing lipid droplets in living cells.
authors.affiliation: Department of Chemistry, Seoul National University, Seoul 08826, Korea. sbpark@snu.ac.kr.
authors.forename: Heejun
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Chemistry, Seoul National University, Seoul 08826, Korea. sbpark@snu.ac.kr.
authors.forename: Ala
authors.initials: A
authors.lastname: Jo
authors.affiliation: WCU Department of Biophysics and Chemical Biology, Seoul National University, Seoul 08826, Korea.
authors.forename: Jaeyoung
authors.initials: J
authors.lastname: Ha
authors.affiliation: Department of Chemistry, Seoul National University, Seoul 08826, Korea. sbpark@snu.ac.kr.
authors.forename: Youngjun
authors.initials: Y
authors.lastname: Lee
authors.affiliation: Department of Chemistry, Seoul National University, Seoul 08826, Korea. sbpark@snu.ac.kr.
authors.forename: Yoon Soo
authors.initials: YS
authors.lastname: Hwang
authors.affiliation: Department of Chemistry, Seoul National University, Seoul 08826, Korea. sbpark@snu.ac.kr and WCU Department of Biophysics and Chemical Biology, Seoul National University, Seoul 08826, Korea.
authors.forename: Seung Bum
authors.initials: SB
authors.lastname: Park