title: An LC-MRM method for measuring intestinal triglyceride assembly using an oral stable isotope-labeled fat challenge.
abstract: OBJECTIVE
  A traditional oral fatty acid challenge assesses absorption of triacylglycerol (TG) into the periphery through the intestines, but cannot distinguish the composition or source of fatty acid in the TG. Stable isotope-labeled tracers combined with LC-MRM can be used to identify and distinguish TG synthesized with dietary and stored fatty acids.
  
  
  RESULTS
  Concentrations of three abundant TGs (52:2, 54:3 and 54:4) were monitored for incorporation of one or two (2)H11-oleate molecules per TG. This method was subjected to routine assay validation and meets typical requirements for an assay to be used to support clinical studies.
  
  
  CONCLUSIONS
  Calculations for the fractional appearance rate of TG in plasma are presented along with the intracellular enterocyte precursor pool for 12 study participants.
authors.affiliation: Merck Research Laboratories, Merck & Co., Inc., Kenilworth, NJ, USA.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Li
authors.affiliation: Center for Human Nutrition, University of Texas Southwestern Medical Center, Dallas, TX, USA.
authors.forename: Elizabeth J
authors.initials: EJ
authors.lastname: Parks
authors.affiliation: Merck Research Laboratories, Merck & Co., Inc., Kenilworth, NJ, USA.
authors.forename: David G
authors.initials: DG
authors.lastname: McLaren
authors.affiliation: Center for Human Nutrition, University of Texas Southwestern Medical Center, Dallas, TX, USA.
authors.forename: Jennifer E
authors.initials: JE
authors.lastname: Lambert
authors.affiliation: Merck Research Laboratories, Merck & Co., Inc., Kenilworth, NJ, USA.
authors.forename: Helene L
authors.initials: HL
authors.lastname: Cardasis
authors.affiliation: Merck Research Laboratories, Merck & Co., Inc., Kenilworth, NJ, USA.
authors.forename: Derek L
authors.initials: DL
authors.lastname: Chappell
authors.affiliation: Merck Research Laboratories, Merck & Co., Inc., Kenilworth, NJ, USA.
authors.forename: Thomas
authors.initials: T
authors.lastname: McAvoy
authors.affiliation: Merck Research Laboratories, Merck & Co., Inc., Kenilworth, NJ, USA.
authors.forename: Gino
authors.initials: G
authors.lastname: Salituro
authors.affiliation: Merck Research Laboratories, Merck & Co., Inc., Kenilworth, NJ, USA.
authors.forename: Achilles
authors.initials: A
authors.lastname: Alon
authors.affiliation: Merck Research Laboratories, Merck & Co., Inc., Kenilworth, NJ, USA.
authors.forename: Justin
authors.initials: J
authors.lastname: Dennie
authors.affiliation: Merck Research Laboratories, Merck & Co., Inc., Kenilworth, NJ, USA.
authors.forename: Manu
authors.initials: M
authors.lastname: Chakravarthy
authors.affiliation: Merck Research Laboratories, Merck & Co., Inc., Kenilworth, NJ, USA.
authors.forename: Sudha S
authors.initials: SS
authors.lastname: Shankar
authors.affiliation: Merck Research Laboratories, Merck & Co., Inc., Kenilworth, NJ, USA.
authors.forename: Omar F
authors.initials: OF
authors.lastname: Laterza
authors.affiliation: Merck Research Laboratories, Merck & Co., Inc., Kenilworth, NJ, USA.
authors.forename: Michael E
authors.initials: ME
authors.lastname: Lassman
mesh_terms: D000293:Adolescent; D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003903:Deuterium / Q000032:analysis; D004032:Diet; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D007553:Isotope Labeling / Q000379:methods; D008297:Male; D019301:Oleic Acid / Q000032:analysis / Q000097:blood / Q000378:metabolism; D014280:Triglycerides / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D055815:Young Adult
chemical_list: D014280:Triglycerides; D019301:Oleic Acid; D003903:Deuterium
keywords: MS; fractional appearance rate; isotope-label; precursor pool; triglycerides