title: Transgelin-2 in B-Cells Controls T-Cell Activation by Stabilizing T Cell - B Cell Conjugates.
abstract: The immunological synapse (IS), a dynamic and organized junction between T-cells and antigen presenting cells (APCs), is critical for initiating adaptive immunity. The actin cytoskeleton plays a major role in T-cell reorganization during IS formation, and we previously reported that transgelin-2, an actin-binding protein expressed in T-cells, stabilizes cortical F-actin, promoting T-cell activation in response to antigen stimulation. Transgelin-2 is also highly expressed in B-cells, although no specific function has been reported. In this study, we found that deficiency in transgelin-2 (TAGLN2-/-) in B-cells had little effect on B-cell development and activation, as measured by the expression of CD69, MHC class II molecules, and CD80/86. Nevertheless, in B-cells, transgelin-2 accumulated in the IS during the interaction with T-cells. These results led us to hypothesize that transgelin-2 may also be involved in IS stability in B-cells, thereby influencing T-cell function. Notably, we found that transgelin-2 deficiency in B-cells reduced T-cell activation, as determined by the release of IL-2 and interferon-γ and the expression of CD69. Furthermore, the reduced T-cell activation was correlated with reduced B-cell-T-cell conjugate formation. Collectively, these results suggest that actin stability in B-cells during IS formation is critical for the initiation of adaptive T-cell immunity.
authors.affiliation: School of Life Sciences, Immune Synapse Research Center and Cell Dynamics Research Center, Gwangju Institute of Science and Technology, Gwangju, Korea.|School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, Korea.
authors.forename: Bo-Ra
authors.initials: BR
authors.lastname: Na
authors.affiliation: School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, Korea.|World Institute of Kimchi, Gwangju, Korea.
authors.forename: Min-Sung
authors.initials: MS
authors.lastname: Kwon
authors.affiliation: School of Life Sciences, Immune Synapse Research Center and Cell Dynamics Research Center, Gwangju Institute of Science and Technology, Gwangju, Korea.|School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, Korea.
authors.forename: Myoung-Won
authors.initials: MW
authors.lastname: Chae
authors.affiliation: School of Life Sciences, Immune Synapse Research Center and Cell Dynamics Research Center, Gwangju Institute of Science and Technology, Gwangju, Korea.|School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, Korea.
authors.forename: Hye-Ran
authors.initials: HR
authors.lastname: Kim
authors.affiliation: School of Life Sciences, Immune Synapse Research Center and Cell Dynamics Research Center, Gwangju Institute of Science and Technology, Gwangju, Korea.|School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, Korea.
authors.forename: Chang-Hyun
authors.initials: CH
authors.lastname: Kim
authors.affiliation: School of Life Sciences, Immune Synapse Research Center and Cell Dynamics Research Center, Gwangju Institute of Science and Technology, Gwangju, Korea.|School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, Korea.
authors.forename: Chang-Duk
authors.initials: CD
authors.lastname: Jun
authors.affiliation: School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, Korea.
authors.forename: Zee-Yong
authors.initials: ZY
authors.lastname: Park
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology; D002460:Cell Line; D005786:Gene Expression Regulation; D055786:Gene Knockout Techniques; D006801:Humans; D054992:Immunological Synapses / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008840:Microfilament Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D009124:Muscle Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology*
chemical_list: D008840:Microfilament Proteins; D009124:Muscle Proteins; C585385:Tagln2 protein, mouse