title: Primary dengue haemorrhagic fever in patients from northeast of Brazil is associated with high levels of interferon-β during acute phase.
abstract: Dengue is an acute febrile disease caused by the mosquito-borne dengue virus (DENV) that according to clinical manifestations can be classified as asymptomatic, mild or severe dengue. Severe dengue cases have been associated with an unbalanced immune response characterised by an over secretion of inflammatory cytokines. In the present study we measured type I interferon (IFN-I) transcript and circulating levels in primary and secondary DENV infected patients. We observed that dengue fever (DF) and dengue haemorrhagic fever (DHF) patients express IFN-I differently. While DF and DHF patients express interferon-α similarly (52,71 ± 7,40 and 49,05 ± 7,70, respectively), IFN- β were associated with primary DHF patients. On the other hand, secondary DHF patients were not able to secrete large amounts of IFN- β which in turn may have influenced the high-level of viraemia. Our results suggest that, in patients from our cohort, infection by DENV serotype 3 elicits an innate response characterised by higher levels of IFN- β in the DHF patients with primary infection, which could contribute to control infection evidenced by the low-level of viraemia in these patients. The present findings may contribute to shed light in the role of innate immune response in dengue pathogenesis.
authors.affiliation: Fundação Oswaldo Cruz, Fundação Oswaldo Cruz, Centro de Pesquisas Aggeu Magalhães, Departamento de Virologia, Recife PE , Brasil, Fundação Oswaldo Cruz, Centro de Pesquisas Aggeu Magalhães, Departamento de Virologia, Recife, PE, Brasil.
authors.forename: Renato Antônio Dos Santos
authors.initials: RA
authors.lastname: Oliveira
authors.affiliation: Fundação Oswaldo Cruz, Fundação Oswaldo Cruz, Centro de Pesquisas Aggeu Magalhães, Departamento de Virologia, Recife PE , Brasil, Fundação Oswaldo Cruz, Centro de Pesquisas Aggeu Magalhães, Departamento de Virologia, Recife, PE, Brasil.
authors.forename: Mayara Marques Carneiro da
authors.initials: MM
authors.lastname: Silva
authors.affiliation: Fundação Oswaldo Cruz, Fundação Oswaldo Cruz, Centro de Pesquisas René Rachou, Departamento de Imunologia, Laboratório de Imunologia Celular e Molecular, Belo Horizonte MG , Brasil, Fundação Oswaldo Cruz, Centro de Pesquisas René Rachou, Departamento de Imunologia, Laboratório de Imunologia Celular e Molecular, Belo Horizonte, MG, Brasil.
authors.forename: Carlos Eduardo
authors.initials: CE
authors.lastname: Calzavara-Silva
authors.affiliation: Fundação Oswaldo Cruz, Fundação Oswaldo Cruz, Centro de Pesquisas Aggeu Magalhães, Departamento de Virologia, Recife PE , Brasil, Fundação Oswaldo Cruz, Centro de Pesquisas Aggeu Magalhães, Departamento de Virologia, Recife, PE, Brasil.
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Silva
authors.affiliation: Fundação Oswaldo Cruz, Fundação Oswaldo Cruz, Centro de Pesquisas Aggeu Magalhães, Departamento de Virologia, Recife PE , Brasil, Fundação Oswaldo Cruz, Centro de Pesquisas Aggeu Magalhães, Departamento de Virologia, Recife, PE, Brasil.
authors.forename: Marli Tenório
authors.initials: MT
authors.lastname: Cordeiro
authors.affiliation: Universidade de Pernambuco, Universidade de Pernambuco, Instituto de Ciências Biológicas, Recife PE , Brasil, Universidade de Pernambuco, Instituto de Ciências Biológicas, Recife, PE, Brasil.
authors.forename: Patrícia Muniz Mendes Freire de
authors.initials: PM
authors.lastname: Moura
authors.affiliation: Universidade de Pernambuco, Universidade de Pernambuco, Faculdade de Ciências Médicas, Recife PE , Brasil, Universidade de Pernambuco, Faculdade de Ciências Médicas, Recife, PE, Brasil.
authors.forename: Paulo Neves
authors.initials: PN
authors.lastname: Baptista
authors.affiliation: Fundação Oswaldo Cruz, Fundação Oswaldo Cruz, Centro de Pesquisas Aggeu Magalhães, Departamento de Virologia, Recife PE , Brasil, Fundação Oswaldo Cruz, Centro de Pesquisas Aggeu Magalhães, Departamento de Virologia, Recife, PE, Brasil.|University of Pittsburgh, Center for Vaccine Research, Department of Infectious Diseases and Microbiology, Pittsburgh PA , US, University of Pittsburgh, Center for Vaccine Research, Department of Infectious Diseases and Microbiology, Pittsburgh, PA, US.
authors.forename: Ernesto Torres de Azevedo
authors.initials: ET
authors.lastname: Marques
authors.affiliation: Fundação Oswaldo Cruz, Fundação Oswaldo Cruz, Centro de Pesquisas Aggeu Magalhães, Departamento de Virologia, Recife PE , Brasil, Fundação Oswaldo Cruz, Centro de Pesquisas Aggeu Magalhães, Departamento de Virologia, Recife, PE, Brasil.
authors.forename: Laura Helena Vega Gonzales
authors.initials: LH
authors.lastname: Gil
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D001938:Brazil; D005260:Female; D006801:Humans; D016899:Interferon-beta / Q000097:blood*; D008297:Male; D019595:Severe Dengue / Q000097:blood* / Q000276:immunology; D055815:Young Adult
chemical_list: D016899:Interferon-beta