title: Spreading of genes encoding enterotoxins, haemolysins, adhesin and biofilm among methicillin resistant Staphylococcus aureus strains with staphylococcal cassette chromosome mec type IIIA isolated from burn patients.
abstract: The emergence of antibiotic-resistant Staphylococcus aureus in particular methicillin-resistant S. aureus (MRSA) is an important concern in burn medical centers either in Iran or worldwide. A total of 128 S. aureus isolates were collected from wound infection of burn patients during June 2013 to June 2014. Multiplex-polymerase chain reaction (MPCR) assay was performed for the characterization of the staphylococcal cassette chromosome mec (SCCmec). Genes encoding virulence factors and biofilm were targeted by PCR. Of 128 S. aureus isolates, 77 (60.1%) isolates were MRSA. Fifty four (70.1%) isolates were identified as SCCmec type IIIA. The most frequently detected toxin genes among MRSA isolates with SCCmec type IIIA were sea (64.1%) and hla (51.8%). The rate of coexistence of sea with hla and sea with hla and hlb was 37% and12.9%, respectively. The sec, eta, tst, pvl, hla and hlb genes were not detected in any of the MRSA isolates. The most prevalent genes encoding biofilm was eno, found in 61.1% of isolates, followed by fib and icaA found in 48.1% and 38.8% of the isolates, respectively. The rate of coexistence of fib + eno + icaA + icaD and fib + eno was 20.3% and 9.2%, respectively. The ebps gene was not detected in any of the isolates. In conclusion, our study indicated that the sea, hla, fib and icaA were most frequent genes encoding virulence factors among MRSA with SCCmec type IIIA isolated from burn wound infection. Moreover, the results of this study shows that the rate of coexistence of genes encoding different virulence factor were high.
authors.affiliation: Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mitra
authors.initials: M
authors.lastname: Motallebi
authors.affiliation: Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Fereshteh
authors.initials: F
authors.lastname: Jabalameli
authors.affiliation: Department of Social Medicine, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
authors.forename: Kheirollah
authors.initials: K
authors.lastname: Asadollahi
authors.affiliation: Department of Microbiology, School of Medicine, Lorestan University of Medical Sciences, Khoramabad, Iran.
authors.forename: Morovat
authors.initials: M
authors.lastname: Taherikalani
authors.affiliation: Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: emaneini@gmail.com.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Emaneini
mesh_terms: D018829:Adhesins, Bacterial / Q000032:analysis* / Q000235:genetics; D018441:Biofilms / Q000254:growth & development*; D002056:Burns / Q000150:complications*; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000235:genetics; D004768:Enterotoxins / Q000032:analysis* / Q000235:genetics; D005798:Genes, Bacterial; D005838:Genotype; D006460:Hemolysin Proteins / Q000032:analysis* / Q000235:genetics; D006801:Humans; D007492:Iran / Q000453:epidemiology; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification / Q000302:isolation & purification / Q000472:pathogenicity* / Q000502:physiology*; D017720:Molecular Epidemiology; D060885:Multiplex Polymerase Chain Reaction; D013207:Staphylococcal Skin Infections / Q000453:epidemiology / Q000382:microbiology*; D037521:Virulence Factors / Q000032:analysis / Q000235:genetics
chemical_list: D018829:Adhesins, Bacterial; D004269:DNA, Bacterial; D004768:Enterotoxins; D006460:Hemolysin Proteins; D037521:Virulence Factors
keywords: Burn; MRSA; MSCRAMMs; SCCmec type IIIA; Toxin genes