title: CpSAT-1, a transcribed satellite sequence from the codling moth, Cydia pomonella.
abstract: Satellite DNA (satDNA) is a non-coding component of eukaryotic genomes, located mainly in heterochromatic regions. Relevance of satDNA began to emerge with accumulating evidence of its potential yet hardly comprehensible role that it can play in the genome of many organisms. We isolated the first satDNA of the codling moth (Cydia pomonella, Tortricidae, Lepidoptera), a species with holokinetic chromosomes and a single large heterochromatic element, the W chromosome in females. The satDNA, called CpSAT-1, is located on all chromosomes of the complement, although in different amounts. Surprisingly, the satellite is almost missing in the heterochromatic W chromosome. Additionally, we isolated mRNA from all developmental stages (1st-5th instar larva, pupa, adult), both sexes (adult male and female) and several tissues (Malpighian tubules, gut, heart, testes, and ovaries) of the codling moth and showed the CpSAT-1 sequence was transcribed in all tested samples. Using CpSAT-1 specific primers we amplified, cloned and sequenced 40 monomers from cDNA and gDNA, respectively. The sequence analysis revealed a high mutation rate and the presence of potentially functional motifs, mainly in non-conserved regions of the monomers. Both the chromosomal distribution and the sequence analysis suggest that CPSAT-1 has no function in the C. pomonella genome.
authors.affiliation: Faculty of Science, University of South Bohemia, Branišovská 1760, 370 05, Ceske Budejovice, Czech Republic.|Institute of Entomology, Biology Centre CAS, Ceske Budejovice, Czech Republic.|Institute of Parasitology, Biology Centre CAS, Ceske Budejovice, Czech Republic.
authors.forename: Pavlína
authors.initials: P
authors.lastname: Věchtová
authors.affiliation: Faculty of Science, University of South Bohemia, Branišovská 1760, 370 05, Ceske Budejovice, Czech Republic.|Institute of Entomology, Biology Centre CAS, Ceske Budejovice, Czech Republic.
authors.forename: Martina
authors.initials: M
authors.lastname: Dalíková
authors.affiliation: Faculty of Science, University of South Bohemia, Branišovská 1760, 370 05, Ceske Budejovice, Czech Republic.|Institute of Entomology, Biology Centre CAS, Ceske Budejovice, Czech Republic.
authors.forename: Miroslava
authors.initials: M
authors.lastname: Sýkorová
authors.affiliation: Institute of Entomology, Biology Centre CAS, Ceske Budejovice, Czech Republic.
authors.forename: Martina
authors.initials: M
authors.lastname: Žurovcová
authors.affiliation: Faculty of Science, University of South Bohemia, Branišovská 1760, 370 05, Ceske Budejovice, Czech Republic.|Institute of Parasitology, Biology Centre CAS, Ceske Budejovice, Czech Republic.
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Füssy
authors.affiliation: Faculty of Science, University of South Bohemia, Branišovská 1760, 370 05, Ceske Budejovice, Czech Republic. vitkova@entu.cas.cz.|Institute of Entomology, Biology Centre CAS, Ceske Budejovice, Czech Republic. vitkova@entu.cas.cz.
authors.forename: Magda
authors.initials: M
authors.lastname: Zrzavá
mesh_terms: D000818:Animals; D059006:Chromosomes, Insect; D018076:DNA, Complementary / Q000235:genetics; D004276:DNA, Satellite; D017344:Genes, Insect; D014644:Genetic Variation; D017404:In Situ Hybridization, Fluorescence; D009036:Moths / Q000145:classification / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary; D004276:DNA, Satellite
keywords: Cydia pomonella; Holokinetic chromosomes; Lepidoptera; Satellite DNA; Sex chromosomes