title: Propagation of Chikungunya Virus Using Mosquito Cells.
abstract: Chikungunya virus (CHIKV) is a mosquito-borne alphavirus that transmits in between a mosquito host vector to a primate host and then back to the mosquito host vector to complete its life cycle. Hence, CHIKV must be able to replicate in both host cellular systems that are genetically and biochemically distinct. The ability to grow and propagate the virus in high titers in the laboratory is fundamentally crucial in order to understand virus replication in different host cellular systems and many other CHIKV research areas. Here, we describe a method on CHIKV propagation using C6/36, a mosquito cell line derived from Aedes albopictus in both serum-containing and serum-free media.
authors.affiliation: Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, 5 Science Drive 2, Singapore, 117597, Singapore.
authors.forename: Swee Kim
authors.initials: SK
authors.lastname: Ang
authors.affiliation: Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, 5 Science Drive 2, Singapore, 117597, Singapore.
authors.forename: Shirley
authors.initials: S
authors.lastname: Lam
authors.affiliation: Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, 5 Science Drive 2, Singapore, 117597, Singapore. justin_chu@nuhs.edu.sg.
authors.forename: Justin Jang Hann
authors.initials: JJ
authors.lastname: Chu
mesh_terms: D000330:Aedes; D018354:Alphavirus Infections / Q000821:virology*; D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D002646:Chikungunya virus / Q000502:physiology*; D003470:Culture Media; D016895:Culture Media, Serum-Free; D014779:Virus Replication
chemical_list: D003470:Culture Media; D016895:Culture Media, Serum-Free
keywords: C6/36; CHIKV; Mosquito cells; Serum-free; Virus propagation