title: Role of UCP2 polymorphisms on dietary intake of obese patients who underwent bariatric surgery.
abstract: Uncoupling protein 2 ( UCP2 ) plays an important role in body weight and energy metabolism and may be related to the control of food consumption. This study aimed to investigate the contribution of UCP2 gene variants on the dietary intake on a population after bariatric surgery. This study enrolled 150 obese patients (body mass index ≥ 35kg m(-2) ) who submitted to Roux-en-Y gastric bypass. Weight (kg), BMI (kg m(-2) ), energy (kcal d(-1) ) and macronutrients intake (g d(-1) ) of preoperative and 1-year postoperative period were collected from medical records. Ala55Val and -866G>A polymorphisms in the UCP2 gene were genotyped through allelic discrimination method in real-time polymerase chain reaction using the TaqMan pre-designed SNP Genotyping Assays kits. Hardy-Weinberg equilibrium, t-test and regression models were performed in statistical analysis (P<0.05).We found an allelic frequency of 0.44 for allele Val and 0.41 for allele A. In the postoperative period, patients with at least one rare allele for polymorphisms and with at least one rare allele for both polymorphisms together (haplotype) present a greater energy and carbohydrate intake, even after adjusting for gender, age and weight. Genetic variants in UCP2 gene were associated with the dietary consumption after Roux-En-Y gastric bypass.
authors.affiliation: Department of Internal Medicine, Faculty of Medicine of Ribeirao Preto, University of São Paulo, RibeirãoPreto, Brazil.
authors.forename: C F
authors.initials: CF
authors.lastname: Nicoletti
authors.affiliation: Department of Internal Medicine, Faculty of Medicine of Ribeirao Preto, University of São Paulo, RibeirãoPreto, Brazil.
authors.forename: B M
authors.initials: BM
authors.lastname: Kimura
authors.affiliation: Department of Internal Medicine, Faculty of Medicine of Ribeirao Preto, University of São Paulo, RibeirãoPreto, Brazil.
authors.forename: B A P
authors.initials: BA
authors.lastname: de Oliveira
authors.affiliation: Department of Internal Medicine, Faculty of Medicine of Ribeirao Preto, University of São Paulo, RibeirãoPreto, Brazil.
authors.forename: M A S
authors.initials: MA
authors.lastname: de Pinhel
authors.affiliation: Department of Surgery and Anatomy, Faculty of Medicine of Ribeirao Preto, University of São Paulo, RibeirãoPreto, Brazil.
authors.forename: W
authors.initials: W
authors.lastname: Salgado
authors.affiliation: Department of Internal Medicine, Faculty of Medicine of Ribeirao Preto, University of São Paulo, RibeirãoPreto, Brazil.
authors.forename: J S
authors.initials: JS
authors.lastname: Marchini
authors.affiliation: Department of Internal Medicine, Faculty of Medicine of Ribeirao Preto, University of São Paulo, RibeirãoPreto, Brazil. carla@fmrp.usp.br.
authors.forename: C B
authors.initials: CB
authors.lastname: Nonino
mesh_terms: D000328:Adult; D000483:Alleles; D001069:Appetite Regulation; D050110:Bariatric Surgery; D015992:Body Mass Index; D001938:Brazil; D003131:Combined Modality Therapy; D004038:Diet, Reducing; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D006801:Humans; D008137:Longitudinal Studies; D008297:Male; D009767:Obesity, Morbid / Q000178:diet therapy* / Q000378:metabolism / Q000601:surgery*; D010349:Patient Compliance; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012189:Retrospective Studies; D012528:Satiety Response; D000071246:Uncoupling Protein 2 / Q000235:genetics* / Q000378:metabolism; D015431:Weight Loss
chemical_list: C000604389:UCP2 protein, human; D000071246:Uncoupling Protein 2
keywords: Bariatric surgery; dietary intake; polymorphism; uncoupling protein 2