title: Accelerated acidification by inoculation with a microbial consortia in a complex open environment.
abstract: Bioaugmentation using microbial consortia is helpful in some anaerobic digestion (AD) systems, but accelerated acidification to produce methane has not been performed effectively with corn stalks and cow dung. In this study, the thermophilic microbial consortia MC1 was inoculated into a complex open environment (unsterilized and sterilized systems) to evaluate the feasibility of bioaugmentation to improve acidification efficiency. The results indicated that MC1 itself degraded lignocellulose efficiently, and accumulated more organic acids within 3days. Similar trends were also observed in the unsterilized system, where the hemicellulose degradation rate and organic acid concentrations increased significantly by two-fold and 20.1% (P<0.05), respectively, and clearly reduced the loss of product. Microbial composition did not change obviously after inoculating MC1, but the abundance of members of MC1, such as Bacillus and Clostridium, increased clearly on day 3. Finally, the acidogenic fluid improved methane yield significantly (P<0.05) via bioaugmentation.
authors.affiliation: Center of Biomass Engineering/College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
authors.forename: Jiadong
authors.initials: J
authors.lastname: Yu
authors.affiliation: Center of Biomass Engineering/College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Center of Biomass Engineering/College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.affiliation: Center of Biomass Engineering/College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
authors.forename: Yubin
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Center of Biomass Engineering/College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
authors.forename: Jingwei
authors.initials: J
authors.lastname: Wu
authors.affiliation: Center of Biomass Engineering/College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
authors.forename: Xufeng
authors.initials: X
authors.lastname: Yuan
authors.affiliation: Center of Biomass Engineering/College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
authors.forename: Wanbin
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Center of Biomass Engineering/College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China. Electronic address: acuizj@cau.edu.cn.
authors.forename: Zongjun
authors.initials: Z
authors.lastname: Cui
mesh_terms: D000818:Animals; D001673:Biodegradation, Environmental; D002417:Cattle; D005243:Feces / Q000737:chemistry / Q000382:microbiology; D008031:Lignin / Q000737:chemistry / Q000378:metabolism; D008697:Methane / Q000378:metabolism; D059013:Microbial Consortia; D014866:Waste Products; D003313:Zea mays / Q000378:metabolism
chemical_list: D014866:Waste Products; C036909:lignocellulose; D008031:Lignin; D008697:Methane
keywords: Acidification; Corn stalks; MC1; Methane production; Microbial consortia