title: Investigation of immunomodulatory and anti-inflammatory effects of eriodictyol through its cellular anti-oxidant activity.
abstract: Many studies have been performed to assess the potential utility of natural products as immunomodulatory agents to enhance host responses against infection or to ameliorate immune-based pathologies. To determine whether eriodictyol has immunomodulatory effects and clarify which types of immune effector cells are stimulated in vitro, we investigated the stimulatory effect of eriodictyol on spleen cells isolated from BALB/c mice. Eriodictyol significantly stimulated splenocyte proliferation. However, only B lymphocytes (not T lymphocytes) could be stimulated by eriodictyol in a dose-related manner. Studies assessing potential effect of eriodictyol on innate immunity reported that eriodictyol enhanced significantly the killing activity of natural killer (NK) cells, T lymphocytes, and macrophages. We also demonstrated that eriodictyol inhibited nitric oxide (NO) production and lysosomal enzyme activity in murine peritoneal macrophages cultured ex-vivo, suggesting a potential anti-inflammatory effect in situ. Eriodictyol revealed also a cellular anti-oxidant activity in splenocytes and macrophages. Furthermore, eriodictyol increased catalase activity in spleen cells. From this data, it can be concluded that eriodictyol exhibited an immunomodulatory effect that could be ascribed in part to a cytoprotective effect related to its anti-oxidant activity.
authors.affiliation: Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Avicenna Street, 5000, Monastir, Tunisia.|Unit of Bioactive and Natural Substances and Biotechnology UR12ES12, Faculty of Pharmacy, University of Monastir, Avicenna Street, 5000, Monastir, Tunisia.
authors.forename: Imen
authors.initials: I
authors.lastname: Mokdad-Bzeouich
authors.affiliation: Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Avicenna Street, 5000, Monastir, Tunisia.|Unit of Bioactive and Natural Substances and Biotechnology UR12ES12, Faculty of Pharmacy, University of Monastir, Avicenna Street, 5000, Monastir, Tunisia.
authors.forename: Nadia
authors.initials: N
authors.lastname: Mustapha
authors.affiliation: Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Avicenna Street, 5000, Monastir, Tunisia.|Unit of Bioactive and Natural Substances and Biotechnology UR12ES12, Faculty of Pharmacy, University of Monastir, Avicenna Street, 5000, Monastir, Tunisia.
authors.forename: Aicha
authors.initials: A
authors.lastname: Sassi
authors.affiliation: Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Avicenna Street, 5000, Monastir, Tunisia.|Unit of Bioactive and Natural Substances and Biotechnology UR12ES12, Faculty of Pharmacy, University of Monastir, Avicenna Street, 5000, Monastir, Tunisia.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Bedoui
authors.affiliation: Laboratory of Biomolecular Engineering, National School of Agronomy and Food Industries, National Polytechnics Institute of Lorraine ENSAIA-INPL, Vandoeuvre les, 54505, Nancy, France.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Ghoul
authors.affiliation: Unit of Bioactive and Natural Substances and Biotechnology UR12ES12, Faculty of Pharmacy, University of Monastir, Avicenna Street, 5000, Monastir, Tunisia.
authors.forename: Kamel
authors.initials: K
authors.lastname: Ghedira
authors.affiliation: Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Avicenna Street, 5000, Monastir, Tunisia. leila.chekir@laposte.net.|Unit of Bioactive and Natural Substances and Biotechnology UR12ES12, Faculty of Pharmacy, University of Monastir, Avicenna Street, 5000, Monastir, Tunisia. leila.chekir@laposte.net.
authors.forename: Leila
authors.initials: L
authors.lastname: Chekir-Ghedira
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical; D044950:Flavanones / Q000494:pharmacology*; D007155:Immunologic Factors / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology; D008264:Macrophages / Q000187:drug effects; D008297:Male; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000096:biosynthesis; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects / Q000276:immunology
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D044950:Flavanones; D007155:Immunologic Factors; D009569:Nitric Oxide; D002374:Catalase; C007619:eriodictyol
keywords: Cellular anti-oxidant activity; Eriodictyol; Immunomodulation