title: Genetic control and regulatory mechanisms of succinoglycan and curdlan biosynthesis in genus Agrobacterium.
abstract: Agrobacterium is a genus of gram-negative bacteria that can produce several typical exopolysaccharides with commercial uses in the food and pharmaceutical fields. In particular, succinoglycan and curdlan, due to their good quality in high yield, have been employed on an industrial scale comparatively early. Exopolysaccharide biosynthesis is a multiple-step process controlled by different functional genes, and various environmental factors cause changes in exopolysaccharide biosynthesis through regulatory mechanisms. In this mini-review, we focus on the genetic control and regulatory mechanisms of succinoglycan and curdlan produced by Agrobacterium. Some key functional genes and regulatory mechanisms for exopolysaccharide biosynthesis are described, possessing a high potential for application in metabolic engineering to modify exopolysaccharide production and physicochemical properties. This review may contribute to the understanding of exopolysaccharide biosynthesis and exopolysaccharide modification by metabolic engineering methods in Agrobacterium.
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin, 150090, People's Republic of China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin, 150090, People's Republic of China. ang.li.harbin@gmail.com.
authors.forename: Ang
authors.initials: A
authors.lastname: Li
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin, 150090, People's Republic of China.
authors.forename: Fang
authors.initials: F
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin, 150090, People's Republic of China. yangxj@hit.edu.cn.
authors.forename: Jixian
authors.initials: J
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin, 150090, People's Republic of China.
authors.forename: Yutong
authors.initials: Y
authors.lastname: Xie
mesh_terms: D060054:Agrobacterium / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D060847:Metabolic Engineering; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D047071:beta-Glucans / Q000378:metabolism*
chemical_list: D011135:Polysaccharides, Bacterial; D047071:beta-Glucans; C038459:curdlan; C056779:succinoglycan
keywords: Agrobacterium; Exopolysaccharide; Genetic control; Regulatory mechanisms