title: Three new 16-membered macrolide compounds from a genetically engineered strain S. avermitilis MHJ1011.
abstract: Three new 16-membered macrolide compounds, 13α-O-α-l-oleandrosyl milbemycin β3 (1), 13α-O-α-l-oleandrosyl-25-ethyl milbemycin β3 (2), 13α-O-α-l-oleandrosyl-25-isopropyl milbemycin β3 (3), were isolated from the genetically engineered strains Streptomyces avermitilis MHJ1011. Their structures were determined on the basis of spectroscopic analysis, including 1D and 2D NMR techniques as well as ESI-MS and comparison with data from the literature. Both compounds 1-3 displayed impressive acaricidal activity against larval mites with the IC50 values of 0.0327, 0.0276 and 0.0235mg/L, respectively, which are higher than those of 13α-hydroxy milbemycin β3 and 13α-hydroxy-25-ethyl milbemycin β3.
authors.affiliation: Provincial Joint Engineering Laboratory of Biopesticide Preparation, School of Forestry & Biotechnology, Zhejiang Agricultural and Forestry University, Lin'An 311300, PR China.
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Pan
authors.affiliation: Provincial Joint Engineering Laboratory of Biopesticide Preparation, School of Forestry & Biotechnology, Zhejiang Agricultural and Forestry University, Lin'An 311300, PR China.
authors.forename: Xu
authors.initials: X
authors.lastname: Wan
authors.affiliation: Provincial Joint Engineering Laboratory of Biopesticide Preparation, School of Forestry & Biotechnology, Zhejiang Agricultural and Forestry University, Lin'An 311300, PR China.
authors.forename: Shao-Yong
authors.initials: SY
authors.lastname: Zhang
authors.affiliation: Department of New Drug Screening, Zhejiang Hisun Pharmaceutical Co., Ltd, Taizhou 318000, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of New Drug Screening, Zhejiang Hisun Pharmaceutical Co., Ltd, Taizhou 318000, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Provincial Joint Engineering Laboratory of Biopesticide Preparation, School of Forestry & Biotechnology, Zhejiang Agricultural and Forestry University, Lin'An 311300, PR China. Electronic address: anlchen@126.com.
authors.forename: An-Liang
authors.initials: AL
authors.lastname: Chen
authors.affiliation: Provincial Joint Engineering Laboratory of Biopesticide Preparation, School of Forestry & Biotechnology, Zhejiang Agricultural and Forestry University, Lin'An 311300, PR China; Department of New Drug Screening, Zhejiang Hisun Pharmaceutical Co., Ltd, Taizhou 318000, PR China. Electronic address: jdwang@hisunpharm.com.
authors.forename: Ji-Dong
authors.initials: JD
authors.lastname: Wang
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D005818:Genetic Engineering; D018942:Macrolides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008925:Mites / Q000187:drug effects*; D008968:Molecular Conformation; D013302:Streptomyces / Q000737:chemistry*; D013329:Structure-Activity Relationship
chemical_list: D018942:Macrolides
keywords: 16-Membered macrolide compounds; Acaricidal activity; Genetically engineered strain S. avermitilis MHJ1011