title: Immunoprecipitation.
abstract: Selective immunoprecipitation of proteins is a useful tool for characterizing proteins and protein-protein interactions. Clear step-by-step protocols are provided for preparing lysates of cells and yeast under a variety of conditions, for binding the antibody to a solid matrix, and for performing the actual immunoprecipitation. An additional method is provided for increasing the specificity of the technique by reprecipitating the antigen with the same or a different antibody. © 2016 by John Wiley & Sons, Inc.
authors.affiliation: National Institute of Child Health and Human Development, Bethesda, Maryland.
authors.forename: Juan S
authors.initials: JS
authors.lastname: Bonifacino
authors.affiliation: National Institute of Child Health and Human Development, Bethesda, Maryland.
authors.forename: David C
authors.initials: DC
authors.lastname: Gershlick
authors.affiliation: National Institute of Child Health and Human Development, Bethesda, Maryland.
authors.forename: Esteban C
authors.initials: EC
authors.lastname: Dell'Angelica
mesh_terms: D000818:Animals; D000906:Antibodies / Q000378:metabolism; D002448:Cell Adhesion; D003902:Detergents; D005898:Glass; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods*; D060328:Magnetic Phenomena; D008863:Microspheres; D011489:Protein Denaturation; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D012996:Solutions
chemical_list: D000906:Antibodies; D003902:Detergents; D012996:Solutions
keywords: agarose bead; antibody; antigen; immunoprecipitation; immunoprecipitation-recapture; magnetic bead