title: Xyloside-primed Chondroitin Sulfate/Dermatan Sulfate from Breast Carcinoma Cells with a Defined Disaccharide Composition Has Cytotoxic Effects in Vitro.
abstract: We previously reported that the xyloside 2-(6-hydroxynaphthyl) β-d-xylopyranoside (XylNapOH), in contrast to 2-naphthyl β-d-xylopyranoside (XylNap), specifically reduces tumor growth both in vitro and in vivo Although there are indications that this could be mediated by the xyloside-primed glycosaminoglycans (GAGs) and that these differ in composition depending on xyloside and cell type, detailed knowledge regarding a structure-function relationship is lacking. In this study we isolated XylNapOH- and XylNap-primed GAGs from a breast carcinoma cell line, HCC70, and a breast fibroblast cell line, CCD-1095Sk, and demonstrated that both XylNapOH- and XylNap-primed chondroitin sulfate/dermatan sulfate GAGs derived from HCC70 cells had a cytotoxic effect on HCC70 cells and CCD-1095Sk cells. The cytotoxic effect appeared to be mediated by induction of apoptosis and was inhibited in a concentration-dependent manner by the XylNap-primed heparan sulfate GAGs. In contrast, neither the chondroitin sulfate/dermatan sulfate nor the heparan sulfate derived from CCD-1095Sk cells primed on XylNapOH or XylNap had any effect on the growth of HCC70 cells or CCD-105Sk cells. These observations were related to the disaccharide composition of the XylNapOH- and XylNap-primed GAGs, which differed between the two cell lines but was similar when the GAGs were derived from the same cell line. To our knowledge this is the first report on cytotoxic effects mediated by chondroitin sulfate/dermatan sulfate.
authors.affiliation: From the Department of Experimental Medical Science and andrea.persson@med.lu.se.
authors.forename: Andrea
authors.initials: A
authors.lastname: Persson
authors.affiliation: From the Department of Experimental Medical Science and.
authors.forename: Emil
authors.initials: E
authors.lastname: Tykesson
authors.affiliation: From the Department of Experimental Medical Science and.
authors.forename: Gunilla
authors.initials: G
authors.lastname: Westergren-Thorsson
authors.affiliation: From the Department of Experimental Medical Science and.
authors.forename: Anders
authors.initials: A
authors.lastname: Malmström
authors.affiliation: the Centre for Analysis and Synthesis, Centre for Chemistry and Chemical Engineering, Lund University, SE-221 84 Lund, Sweden.
authors.forename: Ulf
authors.initials: U
authors.lastname: Ellervik
authors.affiliation: From the Department of Experimental Medical Science and.
authors.forename: Katrin
authors.initials: K
authors.lastname: Mani
mesh_terms: D017209:Apoptosis; D002455:Cell Division; D045744:Cell Line, Tumor; D002809:Chondroitin Sulfates / Q000737:chemistry / Q000378:metabolism*; D003871:Dermatan Sulfate / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D004187:Disaccharides / Q000737:chemistry*; D005260:Female; D006027:Glycosides / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques
chemical_list: D004187:Disaccharides; D006027:Glycosides; C069524:dermatan sulfate chondroitin sulfate; C010807:xylosides; D003871:Dermatan Sulfate; D002809:Chondroitin Sulfates
keywords: cancer; chondroitin sulfate; dermatan sulfate; disaccharide fingerprint; glycosaminoglycan; heparan sulfate; high-performance liquid chromatography (HPLC); structure-function; xyloside