title: [Identification of Volatile Chemical Constituents from Hui Formula "Ha Hei Lili" by GC-MS].
abstract: OBJECTIVE
  The volatile components of the Hui formula "Ha Hei Lili" were extracted by steam distillation extraction (SD) and supercritical CO2 fluid extraction, and the structures were analyzed and identified by GC-MS.
  
  
  METHODS
  The GC-MS conditions were set as follows: Rxi-5Sil MS quartz capillary column (30 m x 0.25 mm, 0.25 μm), the initial temperature of 50 degrees C to keep 1 min, to 10 degrees C/min heating to 120 degrees C, maintained 3 min, then to 3 degrees C/min heating to 200 degrees C, maintained 3 min, and then to 5 degreesC/min heating to 290 degrees C, maintained until completion of analysis; helium as the carrier gas, column flow rate 1.0 ml/min, split ratio 25: 1, inlet temperature 250 degrees C, EI ionization source 70 eV, ion source temperature 230 degrees C, scan range of m/z 35 - 500.
  
  
  RESULTS
  Yield of volatile oil were 0.21% and 5.44% extracted by SD and SFE methods, respectively; and for SD method, 36 kinds of compounds were identified, accounted for 87.02% of total mass of volatile oil; for SFE method, 38 kinds of constituents were identified, accounted for 97.47% of total mass of volatile oil.
  
  
  CONCLUSIONS
  The type of constituents contained in the volatile oil extracted by SD and SFE methods are totally different; and GC-MS can be used to identify the structures and relative content of volatile components, the results of this study can provide an experimental basis for development and utilization of Hui formula "Ha Hei Lili".
authors.forename: Xue-qin
authors.initials: XQ
authors.lastname: Ma
authors.forename: Mei
authors.initials: M
authors.lastname: Wang
authors.forename: Xiao-xue
authors.initials: XX
authors.lastname: Xu
authors.forename: Zhuan-zhuan
authors.initials: ZZ
authors.lastname: Zhang
authors.forename: Wan-nian
authors.initials: WN
authors.lastname: Zhang
mesh_terms: D025924:Chromatography, Supercritical Fluid; D057168:Distillation; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D009822:Oils, Volatile / Q000737:chemistry* / Q000302:isolation & purification; D064209:Phytochemicals / Q000737:chemistry* / Q000302:isolation & purification; D013227:Steam; D013696:Temperature
chemical_list: D004365:Drugs, Chinese Herbal; D009822:Oils, Volatile; D064209:Phytochemicals; D013227:Steam