title: Development of a lateral-flow assay (LFA) for rapid detection of Soybean mosaic virus.
abstract: Soybean mosaic virus (SMV) is the most common virus in soybean and poses a serious threat to crop production and germplasm recession in many countries worldwide. In this study, a highly practical and rapid lateral-flow assay (LFA) was developed for the detection of SMV. The SMV coat protein (CP) was prokaryotically expressed and purified to immunize mice. After generation of hybridoma cell lines, four anti-SMV monoclonal antibodies were selected. The LFA-strip was then assembled using a double-antibody sandwich strategy. When the SMV-infected leaf sample was assayed using the assembled LFA-strip, the positive pink color appeared in the test line within 5-10min. The strip only gave positive results with SMV and not other viruses tested and could be used to detect 800 fold dilutions of infected leaf samples. The LFA could be used to detect SMV in infected leaf tissue as well as soybean seeds. To our knowledge, this is the first report of the development of a LFA for the detection of SMV. The practical, rapid and specific assay that was developed in this study can be widely applied to the diagnosis and surveillance of SMV in the laboratory and the field.
authors.affiliation: Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Wen-Na
authors.initials: WN
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Jin-Yan
authors.initials: JY
authors.lastname: Tian
authors.affiliation: Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Wen-Yang
authors.initials: WY
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Zheng-Qiang
authors.initials: ZQ
authors.lastname: Chen
authors.affiliation: Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Sun
authors.affiliation: Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Xue
authors.affiliation: Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
authors.forename: Xin-Qiu
authors.initials: XQ
authors.lastname: Tan
authors.affiliation: Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Wang
authors.affiliation: Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Feng-Quan
authors.initials: FQ
authors.lastname: Liu
authors.affiliation: Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: taoxiaorong@njau.edu.cn.
authors.forename: Xiao-Rong
authors.initials: XR
authors.lastname: Tao
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000302:isolation & purification; D000914:Antibodies, Viral / Q000276:immunology; D036022:Capsid Proteins / Q000276:immunology; D051379:Mice; D009029:Mosaic Viruses / Q000276:immunology / Q000302:isolation & purification*; D010935:Plant Diseases / Q000821:virology; D018515:Plant Leaves / Q000821:virology; D012639:Seeds / Q000821:virology; D012680:Sensitivity and Specificity; D013025:Glycine max / Q000821:virology*
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D036022:Capsid Proteins
keywords: Anti-SMV monoclonal antibody; Diagnosis; Lateral-flow assay (LFA); Soybean mosaic virus (SMV)