title: Development of a simple assay system for protein-stabilizing efficiency based on hemoglobin protection against denaturation and measurement of the cooperative effect of mixing protein stabilizers.
abstract: We have elucidated the cooperative stabilization of proteins by sugars, amino acids, and other protein-stabilizing agents using a new and simple assay system. Our system determines the protein-stabilizing ability of various compounds by measuring their ability to protect hemoglobin from denaturation. Hemoglobin denaturation was readily measured by quantitative changes in its ultraviolet-visible absorption spectrum. The efficiency of our assay was confirmed using various sugars such as trehalose and sucrose that are known to be good protein stabilizers. We have also found that mixtures of two different types of protein stabilizers resulted in a cooperative stabilizing effect on protein.
authors.affiliation: a Graduate School of Science , Osaka University , Toyonaka , Japan.
authors.forename: Siyu
authors.initials: S
authors.lastname: Chen
authors.affiliation: a Graduate School of Science , Osaka University , Toyonaka , Japan.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Manabe
authors.affiliation: a Graduate School of Science , Osaka University , Toyonaka , Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Minamoto
authors.affiliation: a Graduate School of Science , Osaka University , Toyonaka , Japan.
authors.forename: Naoka
authors.initials: N
authors.lastname: Saiki
authors.affiliation: a Graduate School of Science , Osaka University , Toyonaka , Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Fukase
mesh_terms: D000596:Amino Acids / Q000494:pharmacology; D002241:Carbohydrates / Q000494:pharmacology; D004347:Drug Interactions; D006454:Hemoglobins / Q000737:chemistry*; D011489:Protein Denaturation / Q000187:drug effects*; D055550:Protein Stability / Q000187:drug effects; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D000596:Amino Acids; D002241:Carbohydrates; D006454:Hemoglobins
keywords: hemoglobin; protein denaturation; protein stabilizer; trehalose