title: Proteomic analysis of short-term preload-induced eccentric cardiac hypertrophy.
abstract: Hemodynamic load leads to cardiac hypertrophy and heart failure. While afterload (pressure overload) induces concentric hypertrophy, elevation of preload (volume overload) yields eccentric hypertrophy and is associated with a better outcome. Here we analysed the proteomic pattern of mice subjected to short-term preload.
  
  
  
  Female FVB/N mice were subjected to aortocaval shunt-induced volume overload that leads to an eccentric hypertrophy (left ventricular weight/tibia length +31 %) with sustained systolic heart function at 1 week after operation. Two-dimensional gel electrophoresis (2-DE) followed by mass spectrometric analysis showed alteration in the expression of 25 protein spots representing 21 different proteins. 64 % of these protein spots were up-regulated and 36 % of the protein spots were consistently down-regulated. Interestingly, α-1-antitrypsin was down-regulated, indicating higher elastin degradation and possibly contributing to the early dilatation. In addition to contractile and mitochondrial proteins, polymerase I and transcript release factor protein (PTRF) was also up-regulated, possibly contributing to the preload-induced signal transduction.
  
  
  
  Our findings reveal the proteomic changes of early-stage eccentric myocardial remodeling after volume overload. Induced expression of some of the respiratory chain enzymes suggests a metabolic shift towards an oxidative phosphorylation that might contribute to the favorable remodeling seen in early VO. Down-regulation of α-1-antitrypsin might contribute to extracellular matrix remodeling and left ventricular dilatation. We also identified PTRF as a potential signaling regulator of volume overload-induced cardiac hypertrophy.
authors.affiliation: Department of Cardiology and Pneumology, University Medical Center, Goettingen, Germany.|DZHK (German Centre for Cardiovascular Research), Partner Site Goettingen, Germany.|Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
authors.forename: Belal A
authors.initials: BA
authors.lastname: Mohamed
authors.affiliation: Institute of Clinical Chemistry/UMG-Laboratories, University Medical Center, Goettingen, Germany.
authors.forename: Abdul R
authors.initials: AR
authors.lastname: Asif
authors.affiliation: Department of Cardiology and Pneumology, University Medical Center, Goettingen, Germany.
authors.forename: Moritz
authors.initials: M
authors.lastname: Schnelle
authors.affiliation: Institute of Clinical Chemistry/UMG-Laboratories, University Medical Center, Goettingen, Germany.|Department of Microbiology, Kohat University of Science and Technology, Kohat, Pakistan.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Qasim
authors.affiliation: Department of Cardiology and Pneumology, University Medical Center, Goettingen, Germany.|DZHK (German Centre for Cardiovascular Research), Partner Site Goettingen, Germany.
authors.forename: Sara
authors.initials: S
authors.lastname: Khadjeh
authors.affiliation: Department of Cardiology and Pneumology, University Medical Center, Goettingen, Germany.|DZHK (German Centre for Cardiovascular Research), Partner Site Goettingen, Germany.
authors.forename: Dawid
authors.initials: D
authors.lastname: Lbik
authors.affiliation: Department of Cardiology and Pneumology, University Medical Center, Goettingen, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Schott
authors.affiliation: Department of Cardiology and Pneumology, University Medical Center, Goettingen, Germany.|DZHK (German Centre for Cardiovascular Research), Partner Site Goettingen, Germany.
authors.forename: Gerd
authors.initials: G
authors.lastname: Hasenfuss
authors.affiliation: Department of Cardiology and Pneumology, University Medical Center, Goettingen, Germany. ktoischer@med.uni-goettingen.de.|DZHK (German Centre for Cardiovascular Research), Partner Site Goettingen, Germany. ktoischer@med.uni-goettingen.de.|Abteilung Kardiologie und Pneumologie, Universitätsmedizin Göttingen, Robert-Koch-Str. 40, 37075, Göttingen, Germany. ktoischer@med.uni-goettingen.de.
authors.forename: Karl
authors.initials: K
authors.lastname: Toischer
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002306:Cardiac Volume; D006332:Cardiomegaly / Q000378:metabolism* / Q000473:pathology; D002853:Chromatography, Liquid; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D008297:Male; D051379:Mice; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D060066:Protein Interaction Maps; D020543:Proteome / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D015398:Signal Transduction; D053719:Tandem Mass Spectrometry
chemical_list: D020543:Proteome
keywords: Aortocaval shunt; Eccentric hypertrophy; Heart failure; Preload