title: Platelet-derived factor V promotes angiogenesis in a mouse hind limb ischemia model.
abstract: Coagulation factor V (FV) is distributed in plasma and platelet pools, which are distinguished by physical and functional differences. FV has been extensively studied for its roles in coagulation. The roles of FV in other physiologic pathways remain understudied.
  
  
  
  Hind limb ischemia was produced in transgenic mice by femoral artery ligation, with different levels of FV gene expression restricted to the plasma or platelets.
  
  
  
  Hind limb blood flow perfusion in mice with higher platelet FV was significantly increased. The expression of major angiogenesis-related factors was correlated with the level of FV during ischemia. Furthermore, a platelet depletion and transfusion procedure showed that the transfusion of platelets with higher levels of FV into transgenic mice with undetectable platelet FV significantly rescued the ischemia-mediated impairments in blood flow perfusion. Immunohistochemistry analysis also indicated markedly increased capillary formation in the ischemic muscle of mice with higher platelet FV. Moreover, thrombin activity was significantly higher in the mice with higher platelet FV. Platelets expressing higher levels of FV stimulated increased endothelial cell migration. Hind limb blood flow perfusion was significantly blocked by thrombin inhibitor.
  
  
  
  These findings suggest that platelet-derived FV contributes to the control of angiogenesis and is likely associated with thrombin generation.
authors.affiliation: Drug Discovery Research Center, Southwest Medical University, Luzhou, Sichuan, People's Republic of China; Laboratory for Cardiovascular Pharmacology of the Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Drug Discovery Research Center, Southwest Medical University, Luzhou, Sichuan, People's Republic of China; Laboratory for Cardiovascular Pharmacology of the Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, People's Republic of China.
authors.forename: Lamei
authors.initials: L
authors.lastname: Xiao
authors.affiliation: Drug Discovery Research Center, Southwest Medical University, Luzhou, Sichuan, People's Republic of China; Laboratory for Cardiovascular Pharmacology of the Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, People's Republic of China.
authors.forename: Ni
authors.initials: N
authors.lastname: Chen
authors.affiliation: Drug Discovery Research Center, Southwest Medical University, Luzhou, Sichuan, People's Republic of China; Laboratory for Cardiovascular Pharmacology of the Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, People's Republic of China.
authors.forename: Yongjie
authors.initials: Y
authors.lastname: Li
authors.affiliation: Drug Discovery Research Center, Southwest Medical University, Luzhou, Sichuan, People's Republic of China; Laboratory for Cardiovascular Pharmacology of the Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Deng
authors.affiliation: Drug Discovery Research Center, Southwest Medical University, Luzhou, Sichuan, People's Republic of China; Laboratory for Cardiovascular Pharmacology of the Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, People's Republic of China.
authors.forename: Liqun
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Medicine, University of Missouri School of Medicine, Columbia, Mo.
authors.forename: Hongmin
authors.initials: H
authors.lastname: Sun
authors.affiliation: Drug Discovery Research Center, Southwest Medical University, Luzhou, Sichuan, People's Republic of China; Laboratory for Cardiovascular Pharmacology of the Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, People's Republic of China; Department of Medicine, University of Missouri School of Medicine, Columbia, Mo. Electronic address: wuji@missouri.edu.
authors.forename: Jianbo
authors.initials: J
authors.lastname: Wu
mesh_terms: D042501:Angiogenic Proteins / Q000235:genetics / Q000378:metabolism; D000818:Animals; D000991:Antithrombins / Q000494:pharmacology; D001783:Blood Flow Velocity; D001792:Blood Platelets / Q000378:metabolism*; D002465:Cell Movement; D002478:Cells, Cultured; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000378:metabolism; D005165:Factor V / Q000235:genetics / Q000378:metabolism*; D005838:Genotype; D006614:Hindlimb; D006629:Hirudins / Q000494:pharmacology; D007511:Ischemia / Q000097:blood / Q000235:genetics / Q000503:physiopathology / Q000628:therapy*; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018482:Muscle, Skeletal / Q000098:blood supply*; D018919:Neovascularization, Physiologic; D010641:Phenotype; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D017713:Platelet Transfusion; D012039:Regional Blood Flow; D013917:Thrombin / Q000378:metabolism; D013997:Time Factors
chemical_list: D042501:Angiogenic Proteins; D000991:Antithrombins; D006629:Hirudins; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D005165:Factor V; D013917:Thrombin