title: Effect of ukrain on ischemia/reperfusion-induced kidney injury in rats.
abstract: The aim of this study was to investigate the potential protective effect of ukrain on an experimental kidney injury model induced by ischemia and reperfusion (IR) in rats.
  
  
  
  A total of 24 male Sprague-Dawley rats were equally and randomly separated into three groups as follows: group-1: controls (C; only laparotomy); group 2: renal ischemia-reperfusion (IR; occlusion of the renal artery for 30 min and 2 h of reperfusion); and group 3: ukrain treatment and IR applied group (U + IR; occlusion of the renal artery for 30 min and 2 h of reperfusion; ukrain was intraperitoneally administered 1 h before the IR process).
  
  
  
  Serum total oxidant status (TOS) and total antioxidant status (TAS) levels were measured. The oxidative stress index was determined by calculating the TOS/TAS ratio. TAS serum levels significantly increased, and TOS serum levels also prominently decreased in U + IR group, when compared with the IR group (P < 0.001). Mean NGAL level was remarkably higher in IR group, when compared with the U + IR group (P < 0.001). Caspase-3 messenger RNA (mRNA) expression level increased in IR and decreased in U + IR group (P < 0.001). Bcl-xL serum and mRNA expression levels increased in the U + IR group (P < 0.001). In addition, serum iNOS and mRNA expression levels increased in IR group and decreased in U + IR group (P < 0.001).
  
  
  
  Data established from the present study suggest that ukrain may exhibit protective effect against IR-induced kidney injury and that antioxidant activity primarily modulates this effect.
authors.affiliation: Department of Urology, Faculty of Medicine, Dumlupınar University, Kutahya, Turkey. Electronic address: bekiraras1@gmail.com.
authors.forename: Bekir
authors.initials: B
authors.lastname: Aras
authors.affiliation: Department of Physiology, Faculty of Medicine, Dumlupınar University, Kutahya, Turkey.
authors.forename: Raziye
authors.initials: R
authors.lastname: Akçilar
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Dumlupınar University, Kutahya, Turkey.
authors.forename: F Emel
authors.initials: FE
authors.lastname: Koçak
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Dumlupınar University, Kutahya, Turkey.
authors.forename: Havva
authors.initials: H
authors.lastname: Koçak
authors.affiliation: Department of Pediatric Surgery, Faculty of Medicine, Dumlupınar University, Kutahya, Turkey.
authors.forename: Bircan
authors.initials: B
authors.lastname: Savran
authors.affiliation: Department of Pathology, Faculty of Medicine, Dumlupınar University, Kutahya, Turkey.
authors.forename: Hüseyin
authors.initials: H
authors.lastname: Metineren
authors.affiliation: Department of Pediatry, Faculty of Medicine, Dumlupınar University, Kutahya, Turkey.
authors.forename: Yasin Tuğrul
authors.initials: YT
authors.lastname: Karakuş
authors.affiliation: Department of Urology, Faculty of Medicine, Dumlupınar University, Kutahya, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Yücel
mesh_terms: D058186:Acute Kidney Injury / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001600:Berberine Alkaloids / Q000627:therapeutic use*; D015415:Biomarkers / Q000378:metabolism; D004334:Drug Administration Schedule; D007274:Injections, Intraperitoneal; D008297:Male; D018384:Oxidative Stress; D010617:Phenanthridines / Q000627:therapeutic use*; D020011:Protective Agents / Q000627:therapeutic use*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D016896:Treatment Outcome
chemical_list: D000975:Antioxidants; D001600:Berberine Alkaloids; D015415:Biomarkers; D010617:Phenanthridines; D020011:Protective Agents; C060921:ukrain
keywords: Bax; Ischemia–reperfusion; Kidney; NGAL; Ukrain; iNOS