title: Experimental evidence of the kinetic performance achievable with columns packed with new 1.9μm fully porous particles of narrow particle size distribution.
abstract: Fully porous particles of narrow particle size distribution (nPSD) are now commercially available. In this paper, the kinetic performance of columns packed with these particles (1.9μm, 80Å pore size) has been investigated under typical reversed phase conditions by using a mixture of benzene derivatives as probes. The columns exhibited remarkably high efficiency (in the order of 300,000 theoretical plates per meter) and the possibility to be used at relatively high flow rates without loss of performance. These results contrast with previous studies on the same columns. Indeed we have found column efficiency comparable to that reported in previous work but, on the other hand, we could not observe the same dramatic loss of performance when columns were operated at high flow rates. The results presented in this paper, based on a set of six columns with different geometries (2.1 and 3.0 internal diameter×50, 75 and 100mm length), are not consistent with the previously proposed hypothesis that the unusually low intraparticle diffusion, which would characterize these particles, is the origin of the high efficiency of the columns. In a companion paper [1], a detailed investigation of the different terms leading to band broadening will be performed to point out the major contribution to plate height on nPSD columns.
authors.affiliation: Dept. of Drug Chemistry and Technology, "Sapienza" Università di Roma, P.le A. Moro 5, 00185 Roma, Italy.
authors.forename: Omar H
authors.initials: OH
authors.lastname: Ismail
authors.affiliation: Dept. of Chemistry and Pharmaceutical Sciences, University of Ferrara, via L. Borsari 46, 44121 Ferrara, Italy.
authors.forename: Martina
authors.initials: M
authors.lastname: Catani
authors.affiliation: Dept. of Chemistry and Pharmaceutical Sciences, University of Ferrara, via L. Borsari 46, 44121 Ferrara, Italy.
authors.forename: Luisa
authors.initials: L
authors.lastname: Pasti
authors.affiliation: Dept. of Chemistry and Pharmaceutical Sciences, University of Ferrara, via L. Borsari 46, 44121 Ferrara, Italy. Electronic address: cvz@unife.it.
authors.forename: Alberto
authors.initials: A
authors.lastname: Cavazzini
authors.affiliation: Dept. of Drug Chemistry and Technology, "Sapienza" Università di Roma, P.le A. Moro 5, 00185 Roma, Italy.
authors.forename: Alessia
authors.initials: A
authors.lastname: Ciogli
authors.affiliation: Dept. of Drug Chemistry and Technology, "Sapienza" Università di Roma, P.le A. Moro 5, 00185 Roma, Italy.
authors.forename: Claudio
authors.initials: C
authors.lastname: Villani
authors.affiliation: Dept. of Drug Chemistry and Technology, "Sapienza" Università di Roma, P.le A. Moro 5, 00185 Roma, Italy.
authors.forename: Dorina
authors.initials: D
authors.lastname: Kotoni
authors.affiliation: Dept. of Drug Chemistry and Technology, "Sapienza" Università di Roma, P.le A. Moro 5, 00185 Roma, Italy. Electronic address: francesco.gasparrini@uniroma1.it.
authors.forename: Francesco
authors.initials: F
authors.lastname: Gasparrini
authors.affiliation: Applied Research and Development, Millipore Sigma, 595 North Harrison Road, Bellefonte, PA 16823, USA.
authors.forename: David S
authors.initials: DS
authors.lastname: Bell
mesh_terms: D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods*; D007700:Kinetics; D008962:Models, Theoretical; D010316:Particle Size; D016062:Porosity
keywords: Column efficiency; Narrow particle size distribution (nPSD); Sub-2μm fully porous particles; Ultra high performance liquid chromatography (UHPLC)