title: New CNT/poly(brilliant green) and CNT/poly(3,4-ethylenedioxythiophene) based electrochemical enzyme biosensors.
abstract: A combination of the electroactive polymer poly(brilliant green) (PBG) or conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) with carbon nanotubes to obtain CNT/PBG and CNT/PEDOT modified carbon film electrodes (CFE) has been investigated as a new biosensor platform, incorporating the enzymes glucose oxidase (GOx) as test enzyme, alcohol oxidase (AlcOx) or alcohol dehydrogenase (AlcDH). The sensing parameters were optimized for all biosensors based on CNT/PBG/CFE, CNT/PEDOT/CFE platforms. Under optimized conditions, both GOx biosensors exhibited very similar sensitivities, while in the case of AlcOx and AlcDH biosensors, AlcOx/CNT/PBG/CFE was found to give a higher sensitivity and lower detection limit. The influence of dissolved O2 on oxidase-biosensor performance was investigated and was shown to be different for each enzyme. Comparisons were made with similar reported biosensors, showing the advantages of the new biosensors, and excellent selectivity against potential interferents was successfully demonstrated. Finally, alcohol biosensors were successfully used for the determination of ethanol in alcoholic beverages.
authors.affiliation: Department of Chemistry, Faculty of Sciences and Technology, University of Coimbra, 3004-535 Coimbra, Portugal.
authors.forename: Madalina M
authors.initials: MM
authors.lastname: Barsan
authors.affiliation: Department of Chemistry, Faculty of Sciences and Technology, University of Coimbra, 3004-535 Coimbra, Portugal; Dipartimento di Chimica, Università degli Studi di Milano, Via Golgi 19, 20133 Milano, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Pifferi
authors.affiliation: Dipartimento di Chimica, Università degli Studi di Milano, Via Golgi 19, 20133 Milano, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Falciola
authors.affiliation: Department of Chemistry, Faculty of Sciences and Technology, University of Coimbra, 3004-535 Coimbra, Portugal. Electronic address: cbrett@ci.uc.pt.
authors.forename: Christopher M A
authors.initials: CM
authors.lastname: Brett
mesh_terms: D000426:Alcohol Dehydrogenase / Q000378:metabolism*; D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D015374:Biosensing Techniques; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry*; D013379:Substrate Specificity
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D004800:Enzymes, Immobilized; D011108:Polymers; D000644:Quaternary Ammonium Compounds; C121383:poly(3,4-ethylene dioxythiophene); D000429:Alcohol Oxidoreductases; D000426:Alcohol Dehydrogenase; C019643:alcohol oxidase; C006798:brilliant green
keywords: Alcohol dehydrogenase; Alcohol oxidase; Carbon nanotubes; Glucose oxidase; Poly(3,4-ethylenedioxythiophene); Poly(brilliant green)