title: Prenatal stress alters diazepam withdrawal syndrome and 5HT1A receptor expression in the raphe nuclei of adult rats.
abstract: Early-life events have long-term effects on brain structures and cause behavioral alterations that persist into adulthood. The present experiments were designed to investigate the effects of prenatal stress on diazepam-induced withdrawal syndrome and serotonin-1A (5HT1A) receptor expression in the raphe nuclei of adult offspring. The results of the present study reveal that maternal exposure to chronic footshock stress increased the anxiety-like behavior in the prenatally stressed (PS) animals withdrawn from chronic diazepam (2.5mg/kg/day i.p for 1week). Moreover, prenatal stress induced a down-regulation of 5HT1A mRNA in the raphe nuclei of adult offspring. To our knowledge, this study is the first to demonstrate that maternal exposure to chronic footshock stress enhances diazepam withdrawal symptoms and alters 5HT1A receptor gene expression in the raphe nuclei of adult offspring. Thus, more studies are needed to clarify the mechanisms underlying the decrease of 5HT1A receptors expression in the raphe nuclei of PS rats.
authors.affiliation: Laboratory of Pharmacology, Faculty of Medicine and Pharmacy of Casablanca, 19 Rue Tarik Bnou Ziad, Casablanca, Morocco. Electronic address: Lakehayli.sara@gmail.com.
authors.forename: S
authors.initials: S
authors.lastname: Lakehayli
authors.affiliation: Laboratory of Pharmacology, Faculty of Medicine and Pharmacy of Casablanca, 19 Rue Tarik Bnou Ziad, Casablanca, Morocco.
authors.forename: N
authors.initials: N
authors.lastname: Said
authors.affiliation: Genetics and Molecular Pathology Laboratory, Faculty of Medicine and Pharmacy of Casablanca, 19 Rue Tarik Bnou Ziad, Casablanca, Morocco.
authors.forename: M
authors.initials: M
authors.lastname: El Khachibi
authors.affiliation: Sultan My Slimane University, Fac Sciences & Tecniques Beni-Mellal, Life Sciences, Morocco.
authors.forename: M
authors.initials: M
authors.lastname: El Ouahli
authors.affiliation: Genetics and Molecular Pathology Laboratory, Faculty of Medicine and Pharmacy of Casablanca, 19 Rue Tarik Bnou Ziad, Casablanca, Morocco.
authors.forename: S
authors.initials: S
authors.lastname: Nadifi
authors.affiliation: Laboratory of Pharmacology, Faculty of Medicine and Pharmacy of Casablanca, 19 Rue Tarik Bnou Ziad, Casablanca, Morocco.
authors.forename: F
authors.initials: F
authors.lastname: Hakkou
authors.affiliation: Laboratory of Pharmacology, Faculty of Medicine and Pharmacy of Casablanca, 19 Rue Tarik Bnou Ziad, Casablanca, Morocco.
authors.forename: A
authors.initials: A
authors.lastname: Tazi
mesh_terms: D000818:Animals; D003975:Diazepam / Q000009:adverse effects* / Q000494:pharmacology; D004597:Electroshock; D005260:Female; D018757:GABA Modulators / Q000009:adverse effects* / Q000494:pharmacology; D008297:Male; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D011903:Raphe Nuclei / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D017208:Rats, Wistar; D044282:Receptor, Serotonin, 5-HT1A / Q000378:metabolism; D013315:Stress, Psychological / Q000503:physiopathology; D013375:Substance Withdrawal Syndrome / Q000378:metabolism*
chemical_list: D018757:GABA Modulators; D012333:RNA, Messenger; D044282:Receptor, Serotonin, 5-HT1A; D003975:Diazepam
keywords: 5HT1A receptors; diazepam withdrawal; prenatal stress; raphe nuclei