title: Assay of FAAH Activity.
abstract: Fatty acid amide hydrolase (FAAH) is an intracellular enzyme responsible for the hydrolysis of endogenous anandamide (AEA), a reaction that terminates the biological effects of this lipid mediator. The final products of this reaction are arachidonic acid and ethanolamine. In the method described herein, FAAH activity is measured through the use of a radioactive substrate by quantification of reaction products, that is, [(14)C]-ethanolamine from [(14)C-ethanolamine]-AEA.
authors.affiliation: Department of Experimental Medicine and Surgery, Tor Vergata University of Rome, Rome, Italy. Bari@med.uniroma2.it.
authors.forename: Monica
authors.initials: M
authors.lastname: Bari
authors.affiliation: Center of Integrated Research, Campus Bio-Medico University of Rome, Rome, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Feole
authors.affiliation: Center of Integrated Research, Campus Bio-Medico University of Rome, Rome, Italy.|European Center for Brain Research/IRCCS Santa Lucia Foundation, Rome, Italy.
authors.forename: Mauro
authors.initials: M
authors.lastname: Maccarrone
mesh_terms: D000581:Amidohydrolases / Q000378:metabolism*; D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D001095:Arachidonic Acids / Q000378:metabolism; D001921:Brain / Q000201:enzymology; D063388:Endocannabinoids / Q000378:metabolism; D004789:Enzyme Activation; D057075:Enzyme Assays; D019856:Ethanolamine / Q000378:metabolism; D050356:Lipid Metabolism; D051379:Mice; D053284:Polyunsaturated Alkamides / Q000378:metabolism; D000099252:Fatty Acid Amide Hydrolases
chemical_list: D000581:Amidohydrolases; D016718:Arachidonic Acid; D001095:Arachidonic Acids; D063388:Endocannabinoids; D019856:Ethanolamine; D053284:Polyunsaturated Alkamides; D000099252:Fatty Acid Amide Hydrolases; C078814:anandamide
keywords: AEA; Arachidonic acid; Bioactive lipids; Endocannabinoids; Enzyme; Ethanolamine; FAAH