title: Specific patterns of genetic diversity among aromatic rice varieties in Myanmar.
abstract: BACKGROUND
  After observing peculiar rice varieties in Myanmar, in terms of classification in varietal groups and of grain quality, we focused on Myanmar varieties and analyzed variations at 19 microsatellite loci as well as sequences of the aroma gene BADH2.
  
  
  RESULTS
  Microsatellites were able to retrieve the well-established classification into Indica (isozyme group 1), Japonica (group 6, comprising temperate and tropical forms) and specific groups from the Himalayan foothills including some Aus varieties (group 2) and some aromatic varieties (group 5). They revealed a new cluster of accessions close to, but distinct from, non-Myanmar varieties in group 5. With reference to earlier terminology, we propose to distinguish a group "5A" including group 5 varieties from the Indian subcontinent (South and West Asia) and a group "5B" including most group 5 varieties from Myanmar. In Myanmar varieties, aroma was distributed in group 1 (Indica) and in group 5B. New BADH2 variants were found. Some accessions carried a 43 bp deletion in the 3' UTR that was not completely associated with aroma. Other accessions, all of group 5B, displayed a particular BADH2 allele with a 3 bp insertion and 100% association with aroma.
  
  
  CONCLUSIONS
  With the new group and the new alleles found in Myanmar varieties, our study shows that the Himalayan foothills contain series of non-Indica and non-Japonica varietal types with novel variations for useful traits.
authors.affiliation: Plant Biotechnology Center, Myanma Agriculture Service, Yangon, Myanmar.|Rice Science Center and Rice Gene Discovery, Kasetsart University, Kamphaeng Saen, Nakhon Pathom, 73140, Thailand.
authors.forename: Khin Myo
authors.initials: KM
authors.lastname: Myint
authors.affiliation: Cirad, UMR AGAP, Avenue Agropolis, 34398, Montpellier, France. brigitte.courtois@cirad.fr.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Courtois
authors.affiliation: Cirad, UMR AGAP, Avenue Agropolis, 34398, Montpellier, France.
authors.forename: Ange-Marie
authors.initials: AM
authors.lastname: Risterucci
authors.affiliation: Cirad, UMR AGAP, Avenue Agropolis, 34398, Montpellier, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Frouin
authors.affiliation: Department of Agricultural research, Yezin, Nay Pyi Taw, Myanmar.
authors.forename: Khin
authors.initials: K
authors.lastname: Soe
authors.affiliation: Plant Biotechnology Center, Myanma Agriculture Service, Yangon, Myanmar.
authors.forename: Khin Maung
authors.initials: KM
authors.lastname: Thet
authors.affiliation: Rice Science Center and Rice Gene Discovery, Kasetsart University, Kamphaeng Saen, Nakhon Pathom, 73140, Thailand.
authors.forename: Apichart
authors.initials: A
authors.lastname: Vanavichit
authors.affiliation: Cirad, UMR AGAP, Avenue Agropolis, 34398, Montpellier, France.
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Glaszmann
keywords: Aroma; BADH2; Genetic diversity; Oryza sativa; SSR