title: Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation.
abstract: In this work we studied if circulating immune complexes (CIC) of calves with bronchopneumonia have the capacity to modulate function of peripheral blood leukocytes of healthy cattle. CIC of three month old calves (6 healthy and 6 diseased) were isolated by PEG precipitation. Peripheral blood mononuclear cells (MNCs) and granulocytes from healthy calves and cows were the CIC responder cells in in vitro tests. The most remarkable increase of adhesiveness to polystyrene and ROS synthesis (assessed by NBT test) was detected in cows' granulocytes stimulated with CIC of diseased calves. Results of MTT test showed that CIC of both healthy and diseased calves reduced granulocytes' viability. The strongest effect of inhibition of cows' granulocytes resulted from CIC of diseased calves. CIC only moderately reduced spontaneous viability of calves' MNCs. Again, the strongest effect of CIC isolated from diseased calves was observed. In contrast to the low impact of CIC on non-stimulated cells, their inhibitory effect on viability of mitogen stimulated MNCs was very strong. With CFSE assay we showed that both types of CIC stimulated spontaneous, but inhibited mitogen induced proliferation of calves' MNCs. Propidium iodide staining reviled that CIC increased apoptosis/necrosis of both non-stimulated and mitogen stimulated MNCs. CIC of both healthy and diseased calves modulated the function of peripheral blood MNCs and granulocytes, but a stronger effect of CIC of diseased calves was shown. The age of the donors (calves or cows) of the responder cells, and the activation state of these cells, were also of influence.
authors.affiliation: Institute for Medical Research, University of Belgrade, Dr Subotića 4 POB 39, 11129, Belgrade 102, Serbia.
authors.forename: Marijana
authors.initials: M
authors.lastname: Buač
authors.affiliation: Institute for Medical Research, University of Belgrade, Dr Subotića 4 POB 39, 11129, Belgrade 102, Serbia.
authors.forename: Slavko
authors.initials: S
authors.lastname: Mojsilović
authors.affiliation: Faculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobodjenja 18, 11000 Belgrade, Serbia.
authors.forename: Dušan
authors.initials: D
authors.lastname: Mišić
authors.affiliation: PKB Corporation, Industrijsko naselje BB, 11213 Padinska Skela, Belgrade, Serbia.
authors.forename: Dejan
authors.initials: D
authors.lastname: Vuković
authors.affiliation: Institute for Blood Transfusion of Serbia, Svetog Save 39, 11000 Belgrade, Serbia.
authors.forename: Olivera
authors.initials: O
authors.lastname: Savić
authors.affiliation: Faculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobodjenja 18, 11000 Belgrade, Serbia.
authors.forename: Olivera
authors.initials: O
authors.lastname: Valčić
authors.affiliation: Institute for Medical Research, University of Belgrade, Dr Subotića 4 POB 39, 11129, Belgrade 102, Serbia.
authors.forename: Dragana
authors.initials: D
authors.lastname: Marković
authors.affiliation: Faculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobodjenja 18, 11000 Belgrade, Serbia.
authors.forename: Dragan
authors.initials: D
authors.lastname: Gvozdić
authors.affiliation: Institute for Medical Research, University of Belgrade, Dr Subotića 4 POB 39, 11129, Belgrade 102, Serbia.
authors.forename: Vesna
authors.initials: V
authors.lastname: Ilić
authors.affiliation: Faculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobodjenja 18, 11000 Belgrade, Serbia. Electronic address: nataly@vet.bg.ac.rs.
authors.forename: Natalija
authors.initials: N
authors.lastname: Fratrić
mesh_terms: D000367:Age Factors; D000818:Animals; D000936:Antigen-Antibody Complex / Q000276:immunology*; D001996:Bronchopneumonia / Q000097:blood / Q000276:immunology / Q000662:veterinary*; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000276:immunology*; D005260:Female; D006098:Granulocytes / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology*
chemical_list: D000936:Antigen-Antibody Complex
keywords: Calf bronchopneumonia; Circulating immune complexes; Peripheral blood leukocytes