title: Human umbilical cord blood-mesenchymal stem cells transplantation renovates the ovarian surface epithelium in a rat model of premature ovarian failure: Possible direct and indirect effects.
abstract: This study aimed to isolate mesenchymal stem cells (MSC) from human umbilical cord blood (HCB) and to explore their influence on the ovarian epithelium after paclitaxel-induced ovarian failure. Ninety-five rats were divided into 6 groups: control, paclitaxel, paclitaxel and saline, HCB-MSC-treated for 2 weeks, HCB-MSC-treated for 4 weeks, and HCB-MSC-treated for 6 weeks. HCB cells were studied for CD34, CD44, and Oct ¾ using flow cytometry. Serum levels of FSH and E2 were measured using ELISA, RT-PCR analysis for human gene; beta-actin (ACTB), immunohistochemical analysis for CK 8/18, TGF-ß, PCNA and CASP-3 were performed. We found that ACTB gene was expressed in all rats' ovaries received HCB-MSC. After 4 weeks of transplantation, there was significant reduction in FSH, elevation in E2 levels, stabilization of the surface epithelium morphostasis, an increase in the antral follicle count and increase in integrated densities (ID) of CK 8/18, TGF-ß, and PCNA expressions and decrease in ID of CASP-3 expression. We concluded that HCB-MSC can restore the ovarian function after paclitaxel injection through a direct triggering effect on the ovarian epithelium and/or indirect enrichment of ovarian niche through regulating tissue expression of CK 8/18, TGF-ß and PCNA. These molecules are crucial in regulating folliculogenesis and suppressing CASP-3-induced apoptosis.
authors.affiliation: Department of Obstetrics and Gynecology, Taibah University, Almadinah Almunawarah, Saudi Arabia; Department of Obstetrics and Gynecology, Zagazig University, Zagazig, Egypt.
authors.forename: Amr K
authors.initials: AK
authors.lastname: Elfayomy
authors.affiliation: Department of Anatomy, Taibah University, Almadinah Almunawarah, Saudi Arabia; Department of Anatomy, Mansoura University, Mansoura, Egypt. Electronic address: almasry.shaima@gmail.com.
authors.forename: Shaima M
authors.initials: SM
authors.lastname: Almasry
authors.affiliation: Department of Clinical Biochemistry, Taibah University, Saudi Arabia; Department of Medical Biochemistry, Zagazig University, Egypt.
authors.forename: Shereen A
authors.initials: SA
authors.lastname: El-Tarhouny
authors.affiliation: Department of Anatomy, Taibah University, Almadinah Almunawarah, Saudi Arabia; Department of Anatomy, Tanta University, Tanta, Egypt.
authors.forename: Magda A
authors.initials: MA
authors.lastname: Eldomiaty
mesh_terms: D000071443:12E7 Antigen / Q000097:blood; D000199:Actins / Q000096:biosynthesis / Q000235:genetics; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D053148:Caspase 3 / Q000235:genetics; D036101:Cord Blood Stem Cell Transplantation; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D005786:Gene Expression Regulation; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D010053:Ovary / Q000187:drug effects / Q000254:growth & development* / Q000473:pathology; D017239:Paclitaxel / Q000633:toxicity; D016649:Primary Ovarian Insufficiency / Q000097:blood / Q000139:chemically induced / Q000473:pathology / Q000628:therapy*; D051381:Rats; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D000071443:12E7 Antigen; D000199:Actins; D016212:Transforming Growth Factor beta; D005640:Follicle Stimulating Hormone; D053148:Caspase 3; D017239:Paclitaxel