title: Mitochondrial DNA typing of laser-captured single sperm cells to differentiate individuals in a mixed semen stain.
abstract: The identification of individuals in a mixture of two semen samples usually involves an analysis of autosomal and Y chromosomal short tandem repeats (STR) which can exclude unrelated individuals but cannot achieve the purpose of individual identification. In sperm cells, there are multiple copies of mitochondrial DNAs (mtDNA) which exhibit genetic polymorphisms in different matrilineal-related individuals. Single-cell capture technology can be applied to obtain some single sperm cells in a mixed semen sample, then polymerase chain reaction can be employed to amplify the mtDNA hypervariable region I (HVR I) from each cell. By pooling the cells with the same HVR I sequence, we can obtain the sufficient nuclear DNA for STR typing.
authors.affiliation: China Medical University School of Forensic Medicine, Shenyang, P. R. China.|Department of Forensic Medicine, National Police University of China, Shenyang, P. R. China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: China Medical University School of Forensic Medicine, Shenyang, P. R. China.
authors.forename: Mei
authors.initials: M
authors.lastname: Ding
authors.affiliation: China Medical University School of Forensic Medicine, Shenyang, P. R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Pang
authors.affiliation: China Medical University School of Forensic Medicine, Shenyang, P. R. China.
authors.forename: Jiaxin
authors.initials: J
authors.lastname: Xing
authors.affiliation: China Medical University School of Forensic Medicine, Shenyang, P. R. China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Xuan
authors.affiliation: Shenyang Medical College, Shenyang, P. R. China.
authors.forename: Chunhong
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Forensic Medicine, National Police University of China, Shenyang, P. R. China.
authors.forename: Ziqing
authors.initials: Z
authors.lastname: Lin
authors.affiliation: Shenyang Medical College, Shenyang, P. R. China.
authors.forename: Song
authors.initials: S
authors.lastname: Han
authors.affiliation: Department of Forensic Medicine, National Police University of China, Shenyang, P. R. China.
authors.forename: Kewei
authors.initials: K
authors.lastname: Liang
authors.affiliation: China Medical University School of Forensic Medicine, Shenyang, P. R. China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Li
authors.affiliation: China Medical University School of Forensic Medicine, Shenyang, P. R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yao
authors.affiliation: China Medical University School of Forensic Medicine, Shenyang, P. R. China.
authors.forename: Baojie
authors.initials: B
authors.lastname: Wang
mesh_terms: D016172:DNA Fingerprinting / Q000379:methods*; D004272:DNA, Mitochondrial / Q000032:analysis* / Q000235:genetics; D006801:Humans; D008297:Male; D052898:Optical Tweezers; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012661:Semen / Q000166:cytology*; D059010:Single-Cell Analysis; D013094:Spermatozoa / Q000166:cytology*
chemical_list: D004272:DNA, Mitochondrial
keywords: Individual identification; Mixed semen stain; Single-cell capture; mtDNA