title: Staphylococcus aureus Regulatory RNAs as Potential Biomarkers for Bloodstream Infections.
abstract: Staphylococcus aureus is a commensal bacterium and pathogen. Identifying biomarkers for the transition from colonization to disease caused by this organism would be useful. Several S. aureus small RNAs (sRNAs) regulate virulence. We investigated presence and expression of 8 sRNAs in 83 S. aureus strains from 42 patients with sepsis or septic shock and 41 asymptomatic colonized carriers. Small pathogenicity island sRNAs sprB and sprC were clade specific. Six sRNAs had variable expression not correlated with clinical status. Expression of RNAIII was lower in strains from septic shock patients than in strains from colonized patients. When RNAIII was associated with expression of sprD, colonizing strains could be discriminated from strains in patients with bloodstream infections, including patients with sepsis and septic shock. Isolates associated with colonization might have sRNAs with target expression different from those of disease isolates. Monitoring expression of RNAIII and sprD could help determine severity of bloodstream infections.
authors.forename: Valérie
authors.initials: V
authors.lastname: Bordeau
authors.forename: Anne
authors.initials: A
authors.lastname: Cady
authors.forename: Matthieu
authors.initials: M
authors.lastname: Revest
authors.forename: Octavie
authors.initials: O
authors.lastname: Rostan
authors.forename: Mohamed
authors.initials: M
authors.lastname: Sassi
authors.forename: Pierre
authors.initials: P
authors.lastname: Tattevin
authors.forename: Pierre-Yves
authors.initials: PY
authors.lastname: Donnio
authors.forename: Brice
authors.initials: B
authors.lastname: Felden
mesh_terms: D058070:Asymptomatic Diseases; D016470:Bacteremia / Q000382:microbiology*; D001426:Bacterial Proteins / Q000235:genetics; D015415:Biomarkers; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D058885:Multilocus Sequence Typing; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012367:RNA, Viral; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D037521:Virulence Factors / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D015415:Biomarkers; D012329:RNA, Bacterial; D012367:RNA, Viral; C096035:RNAIII, Staphylococcus aureus; D037521:Virulence Factors
keywords: RNAIII; S. aureus protein A typing; Staphylococcus aureus; bacteria; biomarkers; bloodstream infections; immune evasion protein; multilocus sequence typing; nasal carrier; nasal colonization; second immunoglobulin-binding protein; sepsis; septic shock; small RNAs; small pathogenicity island RNAs; spa typing; spr RNAs; staphylococci; transfer–messenger RNA