title: Construction and characterization of a full-length infectious cDNA clone of foot-and-mouth disease virus strain O/JPN/2010 isolated in Japan in 2010.
abstract: A full-length infectious cDNA clone of the genome of a foot-and-mouth disease virus isolated from the 2010 epidemic in Japan was constructed and designated pSVL-f02. Transfection of Cos-7 or IBRS-2 cells with this clone allowed the recovery of infectious virus. The recovered virus had the same in vitro characterization as the parental virus with regard to antigenicity in neutralization and indirect immunofluorescence tests, plaque size and one-step growth. Pigs were experimentally infected with the parental virus or the recombinant virus recovered from pSVL-f02 transfected cells. There were no significant differences in clinical signs or antibody responses between the two groups, and virus isolation and viral RNA detection from clinical samples were similar. Virus recovered from transfected cells therefore retained the in vitro characteristics and the in vivo pathogenicity of their parental strain. This cDNA clone should be a valuable tool to analyze determinants of pathogenicity and mechanisms of virus replication, and to develop genetically engineered vaccines against foot-and-mouth disease virus.
authors.affiliation: Exotic Disease Research Division, National Institute of Animal Health, National Agriculture and Food Research Organization, 6-20-1, Josui-honcho, Kodaira, Tokyo 187-0022, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Nishi
authors.affiliation: Exotic Disease Research Division, National Institute of Animal Health, National Agriculture and Food Research Organization, 6-20-1, Josui-honcho, Kodaira, Tokyo 187-0022, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Onozato
authors.affiliation: Exotic Disease Research Division, National Institute of Animal Health, National Agriculture and Food Research Organization, 6-20-1, Josui-honcho, Kodaira, Tokyo 187-0022, Japan.
authors.forename: Seiichi
authors.initials: S
authors.lastname: Ohashi
authors.affiliation: Exotic Disease Research Division, National Institute of Animal Health, National Agriculture and Food Research Organization, 6-20-1, Josui-honcho, Kodaira, Tokyo 187-0022, Japan.
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Fukai
authors.affiliation: Exotic Disease Research Division, National Institute of Animal Health, National Agriculture and Food Research Organization, 6-20-1, Josui-honcho, Kodaira, Tokyo 187-0022, Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Yamada
authors.affiliation: Exotic Disease Research Division, National Institute of Animal Health, National Agriculture and Food Research Organization, 6-20-1, Josui-honcho, Kodaira, Tokyo 187-0022, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Morioka
authors.affiliation: Exotic Disease Research Division, National Institute of Animal Health, National Agriculture and Food Research Organization, 6-20-1, Josui-honcho, Kodaira, Tokyo 187-0022, Japan. Electronic address: kannot@affrc.go.jp.
authors.forename: Toru
authors.initials: T
authors.lastname: Kanno
mesh_terms: D000276:Adjuvants, Immunologic / Q000235:genetics; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000276:immunology*; D005536:Foot-and-Mouth Disease / Q000276:immunology / Q000821:virology*; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics / Q000276:immunology*; D007564:Japan; D013552:Swine; D013553:Swine Diseases / Q000276:immunology* / Q000821:virology; D015164:Vaccines, Inactivated / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D018076:DNA, Complementary; D015164:Vaccines, Inactivated; D014765:Viral Vaccines
keywords: Foot-and-mouth disease virus; Full-length cDNA; O/JPN/2010 strain; Pathogenicity