title: Fluoride-Induced Oxidative and Inflammatory Stress in Osteosarcoma Cells: Does It Affect Bone Development Pathway?
abstract: Oxidative stress is reported to negatively affect osteoblast cells. Present study reports oxidative and inflammatory signatures in fluoride-exposed human osteosarcoma (HOS) cells, and their possible association with the genes involved in osteoblastic differentiation and bone development pathways. HOS cells were challenged with sublethal concentration (8 mg/L) of sodium fluoride for 30 days and analyzed for transcriptomic expression. In total, 2632 transcripts associated with several biological processes were found to be differentially expressed. Specifically, genes involved in oxidative stress, inflammation, osteoblastic differentiation, and bone development pathways were found to be significantly altered. Variation in expression of key genes involved in the abovementioned pathways was validated through qPCR. Expression of serum amyloid A1 protein, a key regulator of stress and inflammatory pathways, was validated through western blot analysis. This study provides evidence that chronic oxidative and inflammatory stress may be associated with the fluoride-induced impediment in osteoblast differentiation and bone development.
authors.affiliation: Environmental Health Division, CSIR-National Environmental Engineering Research Institute (NEERI), Nagpur, India.
authors.forename: Deepa
authors.initials: D
authors.lastname: Gandhi
authors.affiliation: Environmental Health Division, CSIR-National Environmental Engineering Research Institute (NEERI), Nagpur, India.
authors.forename: Pravin K
authors.initials: PK
authors.lastname: Naoghare
authors.affiliation: Environmental Health Division, CSIR-National Environmental Engineering Research Institute (NEERI), Nagpur, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Bafana
authors.affiliation: Environmental Health Division, CSIR-National Environmental Engineering Research Institute (NEERI), Nagpur, India.
authors.forename: Krishnamurthi
authors.initials: K
authors.lastname: Kannan
authors.affiliation: Environmental Health Division, CSIR-National Environmental Engineering Research Institute (NEERI), Nagpur, India. ss_devi@neeri.res.in.
authors.forename: Saravanadevi
authors.initials: S
authors.lastname: Sivanesan
mesh_terms: D001846:Bone Development / Q000187:drug effects*; D045744:Cell Line, Tumor; D005459:Fluorides / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D007249:Inflammation / Q000378:metabolism / Q000473:pathology; D012516:Osteosarcoma / Q000378:metabolism* / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects*; D012969:Sodium Fluoride / Q000494:pharmacology*
chemical_list: D012969:Sodium Fluoride; D005459:Fluorides
keywords: Bone development; Gene microarray; Inflammatory stress; Osteosarcoma cells (HOS); Oxidative stress; Sodium fluoride