title: Gomafu lncRNA knockout mice exhibit mild hyperactivity with enhanced responsiveness to the psychostimulant methamphetamine.
abstract: The long noncoding RNA Gomafu/MIAT/Rncr2 is thought to function in retinal cell specification, stem cell differentiation and the control of alternative splicing. To further investigate physiological functions of Gomafu, we created mouse knockout (KO) model that completely lacks the Gomafu gene. The KO mice did not exhibit any developmental deficits. However, behavioral tests revealed that the KO mice are hyperactive. This hyperactive behavior was enhanced when the KO mice were treated with the psychostimulant methamphetamine, which was associated with an increase in dopamine release in the nucleus accumbens. RNA sequencing analyses identified a small number of genes affected by the deficiency of Gomafu, a subset of which are known to have important neurobiological functions. These observations suggest that Gomafu modifies mouse behavior thorough a mild modulation of gene expression and/or alternative splicing of target genes.
authors.affiliation: RNA Biology Laboratory, RIKEN, 2-1 Hirosawa, Wako 351-0198, Japan.
authors.forename: Joanna Y
authors.initials: JY
authors.lastname: Ip
authors.affiliation: RNA Biology Laboratory, RIKEN, 2-1 Hirosawa, Wako 351-0198, Japan.
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Sone
authors.affiliation: RNA Biology Laboratory, RIKEN, 2-1 Hirosawa, Wako 351-0198, Japan.
authors.forename: Chieko
authors.initials: C
authors.lastname: Nashiki
authors.affiliation: Banting and Best Department of Medical Research, Donnelly Centre, University of Toronto, Toronto, Ontario M5S 3E1, Canada.
authors.forename: Qun
authors.initials: Q
authors.lastname: Pan
authors.affiliation: Laboratory of Pharmaceutics, Department of Biomedical Pharmaceutics, Gifu Pharmaceutical University, 1-25-4 Daigakunishi, Gifu 501-1196, Japan.
authors.forename: Kiyoyuki
authors.initials: K
authors.lastname: Kitaichi
authors.affiliation: RNA Biology Laboratory, RIKEN, 2-1 Hirosawa, Wako 351-0198, Japan.
authors.forename: Kaori
authors.initials: K
authors.lastname: Yanaka
authors.affiliation: Laboratories of Animal Resource Development and Genetic Engineering, RIKEN Center for Life Science Technologies, 2-2-3 Minatojima Minami, Chuou-ku, Kobe 650-0047, Japan.
authors.forename: Takaya
authors.initials: T
authors.lastname: Abe
authors.affiliation: Section of Behavior Patterns, Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, Okazaki, Japan.|Division of Animal Resources and Development, Life Science Research Center, University of Toyama, 2630 Sugitani, Toyama, 930-0194, Japan.
authors.forename: Keizo
authors.initials: K
authors.lastname: Takao
authors.affiliation: Section of Behavior Patterns, Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, Okazaki, Japan.|Division of Systems Medical Science, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Miyakawa
authors.affiliation: Banting and Best Department of Medical Research, Donnelly Centre, University of Toronto, Toronto, Ontario M5S 3E1, Canada.|Department of Molecular Genetics, University of Toronto, Toronto, Ontario M5S 1A8, Canada.
authors.forename: Benjamin J
authors.initials: BJ
authors.lastname: Blencowe
authors.affiliation: RNA Biology Laboratory, RIKEN, 2-1 Hirosawa, Wako 351-0198, Japan.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Nakagawa
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D000067290:Behavior Rating Scale; D002478:Cells, Cultured; D004298:Dopamine / Q000378:metabolism; D020869:Gene Expression Profiling; D055786:Gene Knockout Techniques; D053263:Gene Regulatory Networks; D006948:Hyperkinesis / Q000235:genetics* / Q000378:metabolism / Q000523:psychology*; D008694:Methamphetamine / Q000008:administration & dosage*; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D009714:Nucleus Accumbens / Q000378:metabolism; D062085:RNA, Long Noncoding / Q000235:genetics*; D017423:Sequence Analysis, RNA; D013566:Sympathomimetics / Q000008:administration & dosage*
chemical_list: C517383:Miat long non-coding RNA; D062085:RNA, Long Noncoding; D013566:Sympathomimetics; D008694:Methamphetamine; D004298:Dopamine