title: Interleukin-6, intracellular adhesion molecule-1, and glycodelin A levels in serum and peritoneal fluid as biomarkers for endometriosis.
abstract: OBJECTIVE
  To compare levels of interleukin-6 (IL-6), intracellular adhesion molecule-1 (ICAM-1), and glycodelin A in serum and peritoneal fluid of patients with and without endometriosis, and to correlate levels with disease stage.
  
  
  METHODS
  An observational study was undertaken at Mansoura University Hospital, Egypt, between March 2014 and June 2015. Patients aged 21-48 years laparoscopically diagnosed with endometriosis and those without endometriosis who underwent laparoscopy for tubal ligation were included. Levels of IL-6, ICAM-1, and glycodelin A were measured in samples of serum and peritoneal fluid. Receiver operating characteristic curves were used to evaluate diagnostic accuracy.
  
  
  RESULTS
  Forty-eight women with endometriosis and 20 without the disorder were included. IL-6 and glycodelin A levels in serum and peritoneal fluid were higher in the endometriosis group than in the control group (P<0.001 for all); ICAM-1 levels did not differ. The sensitivity and specificity values were 93.8% and 80.0% for serum IL-6, 58.3% and 60.0% for serum ICAM-1, and 91.7% and 75.0% for serum glycodelin A. The corresponding values for peritoneal fluid markers were 85.4% and 89.0%, 60.4% and 50.0%, and 89.6% and 90.0%, respectively. IL-6 and glycodelin A levels in serum and peritoneal fluid increased with disease stage (P<0.001 for all).
  
  
  CONCLUSIONS
  IL-6 and glycodelin A, but not ICAM-1, are potential biomarkers for endometriosis and are positively correlated with the disease stage.
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, Mansoura University, Mansoura, Egypt. Electronic address: alaamosbah@hotmail.com.
authors.forename: Alaa
authors.initials: A
authors.lastname: Mosbah
authors.affiliation: Department of Medical Microbiology and Immunology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
authors.forename: Yasmin
authors.initials: Y
authors.lastname: Nabiel
authors.affiliation: Department of Public Health and Community Medicine, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
authors.forename: Eman
authors.initials: E
authors.lastname: Khashaba
mesh_terms: D000328:Adult; D001202:Ascitic Fluid / Q000378:metabolism; D015415:Biomarkers / Q000097:blood / Q000378:metabolism*; D004715:Endometriosis / Q000097:blood / Q000175:diagnosis* / Q000378:metabolism; D005260:Female; D000071816:Glycodelin / Q000097:blood / Q000378:metabolism; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood / Q000378:metabolism; D015850:Interleukin-6 / Q000097:blood / Q000378:metabolism; D008875:Middle Aged; D011237:Predictive Value of Tests; D055815:Young Adult
chemical_list: D015415:Biomarkers; D000071816:Glycodelin; D015850:Interleukin-6; D018799:Intercellular Adhesion Molecule-1
keywords: Endometriosis; Enzyme-linked immunosorbent assay; Glycodelin A; ICAM-1; Interleukin-6; Nonsurgical diagnostic methods