title: Generation of Recombinant Antibodies Against Toxins and Viruses by Phage Display for Diagnostics and Therapy.
abstract: Antibody phage display is an in vitro technology to generate recombinant antibodies. In particular for pathogens like viruses or toxins, antibody phage display is an alternative to hybridoma technology, since it circumvents the limitations of the immune system. Phage display allows the generation of human antibodies from naive antibody gene libraries when either immunized patients are not available or immunization is not ethically feasible. This technology also allows the construction of immune libraries to select in vivo affinity matured antibodies if immunized patients or animals are available.In this review, we describe the generation of human and human-like antibodies from naive antibody gene libraries and antibodies from immune antibody gene libraries. Furthermore, we give an overview about phage display derived recombinant antibodies against viruses and toxins for diagnostics and therapy.
authors.affiliation: Abteilung Biotechnologie, Institut für Biochemie, Biotechnologie und Bioinformatik, Technische Universität Braunschweig, Spielmannstr. 7, 38106, Braunschweig, Germany.
authors.forename: Tobias
authors.initials: T
authors.lastname: Unkauf
authors.affiliation: Abteilung Biotechnologie, Institut für Biochemie, Biotechnologie und Bioinformatik, Technische Universität Braunschweig, Spielmannstr. 7, 38106, Braunschweig, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Miethe
authors.affiliation: Abteilung Biotechnologie, Institut für Biochemie, Biotechnologie und Bioinformatik, Technische Universität Braunschweig, Spielmannstr. 7, 38106, Braunschweig, Germany.
authors.forename: Viola
authors.initials: V
authors.lastname: Fühner
authors.affiliation: Abteilung Biotechnologie, Institut für Biochemie, Biotechnologie und Bioinformatik, Technische Universität Braunschweig, Spielmannstr. 7, 38106, Braunschweig, Germany.|YUMAB GmbH, Rebenring 33, 38106, Braunschweig, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Schirrmann
authors.affiliation: Abteilung Biotechnologie, Institut für Biochemie, Biotechnologie und Bioinformatik, Technische Universität Braunschweig, Spielmannstr. 7, 38106, Braunschweig, Germany.|YUMAB GmbH, Rebenring 33, 38106, Braunschweig, Germany.
authors.forename: André
authors.initials: A
authors.lastname: Frenzel
authors.affiliation: Abteilung Biotechnologie, Institut für Biochemie, Biotechnologie und Bioinformatik, Technische Universität Braunschweig, Spielmannstr. 7, 38106, Braunschweig, Germany. m.hust@tu-bs.de.
authors.forename: Michael
authors.initials: M
authors.lastname: Hust
mesh_terms: D000818:Animals; D061067:Antibodies, Monoclonal, Humanized / Q000096:biosynthesis* / Q000302:isolation & purification*; D061505:Cell Surface Display Techniques / Q000379:methods*; D006801:Humans; D007114:Immunization; D009369:Neoplasms / Q000175:diagnosis / Q000628:therapy; D019151:Peptide Library; D015202:Protein Engineering / Q000379:methods; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D014118:Toxins, Biological / Q000276:immunology*; D014780:Viruses / Q000276:immunology*
chemical_list: D061067:Antibodies, Monoclonal, Humanized; D019151:Peptide Library; D011994:Recombinant Proteins; D014118:Toxins, Biological
keywords: Antibody; Antibody engineering; Pathogen; Phage display; Therapy; Toxins; Viruses; scFv