title: Identification and characterization of ovary development-related protein EJO1 (Eri s 2) from the ovary of Eriocheir sinensis as a new food allergen.
abstract: Crab is a major source of shellfish allergens. Most studies have focused on allergens in crab muscle (CM) rather than on allergens in crab ovary (CO). This study aimed to identify potential allergens in CO from Eriocheir sinensis.
  
  
  
  Dot blot and immunoblotting results revealed the differential reactivity of CM and CO extracts to positive sera from patients allergic to crabs. Four CO proteins showed good specific IgE-binding activities in 2-DE/immunoblotting analysis; mass spectrometry identified the proteins as hemocyanin, vitellogenin, ovary development-related protein EJO1and EJO2. The recently identified allergen EJO1 is named 'Eri s 2' in the World Health Organization and International Union of Immunological Societies (WHO/IUIS) allergen nomenclature database. Recombinant Eri s 2 exhibited good reactivity to positive sera, and pre-incubation with recombinant Eri s 2 abrogated the reactivity of positive sera from two patients to CO in a dose-dependent manner. Moreover, co-incubation of recombinant Eri s 2 with patient basophils dose-dependently promoted basophil activation, confirming the biological activity of Eri s 2.
  
  
  
  CO tissue is an important allergen source, and Eri s 2 is a new crab allergen. This study provides insights that will be useful for component-resolved diagnostics for crab allergy.
authors.affiliation: School of Medical Laboratory, Tianjin Medical University, Tianjin, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Zhu
authors.affiliation: School of Medical Laboratory, Tianjin Medical University, Tianjin, China.
authors.forename: Tiantian
authors.initials: T
authors.lastname: She
authors.affiliation: School of Medical Laboratory, Tianjin Medical University, Tianjin, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Medical Laboratory, Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital, Tianjin, China.
authors.forename: Shaoshen
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Medical Laboratory, Tianjin Chest Hospital, Tianjin, China.
authors.forename: Zixian
authors.initials: Z
authors.lastname: Xu
authors.affiliation: School of Medical Laboratory, Tianjin Medical University, Tianjin, China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Yan
authors.affiliation: School of Medical Laboratory, Tianjin Medical University, Tianjin, China. lhq@tmu.edu.cn.
authors.forename: Huiqiang
authors.initials: H
authors.lastname: Li
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000485:Allergens / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D000818:Animals; D001491:Basophils / Q000276:immunology; D015153:Blotting, Western; D003386:Brachyura / Q000737:chemistry / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007223:Infant; D008297:Male; D008875:Middle Aged; D010053:Ovary / Q000737:chemistry* / Q000276:immunology; D000067208:Shellfish Hypersensitivity / Q000276:immunology*
chemical_list: D000485:Allergens
keywords: Crab ovary; Eriocheir sinensis; Food allergen; Ovary development-related protein EJO1; Recombinant expression