title: Preparative Purification of Anti-Proliferative Diarylheptanoids from Betula platyphylla by High-Speed Counter-Current Chromatography.
abstract: A simple and rapid method using high-speed counter-current chromatography (HSCCC), along with bioassay-guided fractionation based on the anti-proliferative activity against renal and colon cancer cells, has been developed for the preparative separation of aceroside VIII (1) and platyphylloside (2) from Betula platyphylla. A solvent system composed of ethyl acetate/acetonitrile/water (1:0.1:1, v/v/v) was optimized for the separation. The upper phase was used as the stationary phase, and the lower phase was used as the mobile phase. Among these isolated diarylheptanoids, platyphylloside (2) showed anti-proliferative activity in the COLO205 and KM12 colon cells and renal cancer cell lines A498, U031, as well as in MG63 and MG 63.3 osteosarcoma cells. In addition, it showed dose dependent inhibitory effects in the NCI 60 cell line assay. These results suggest that the diarylheptanoids isolated from B. platyphylla with an efficient HSCCC method could be potential multi-targeted therapeutic agents for cancer.
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul 08826, Korea. cnamki@naver.com.|Molecular Targets Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, MD 21702-1202, USA. cnamki@naver.com.
authors.forename: Namki
authors.initials: N
authors.lastname: Cho
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul 08826, Korea. kimkami2@snu.ac.kr.
authors.forename: Hyun Woo
authors.initials: HW
authors.lastname: Kim
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul 08826, Korea. gtato311@snu.ac.kr.
authors.forename: Tae Bum
authors.initials: TB
authors.lastname: Kim
authors.affiliation: Molecular Targets Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, MD 21702-1202, USA. ransomt@mail.nih.gov.
authors.forename: Tanya T
authors.initials: TT
authors.lastname: Ransom
authors.affiliation: Molecular Targets Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, MD 21702-1202, USA. beutlerj@mail.nih.gov.
authors.forename: John A
authors.initials: JA
authors.lastname: Beutler
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul 08826, Korea. shsung@snu.ac.kr.
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Sung
mesh_terms: D029662:Betula / Q000737:chemistry*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D003110:Colonic Neoplasms / Q000188:drug therapy; D036381:Diarylheptanoids / Q000008:administration & dosage / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D007680:Kidney Neoplasms / Q000188:drug therapy; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry; D012997:Solvents / Q000737:chemistry
chemical_list: D036381:Diarylheptanoids; D010936:Plant Extracts; D012997:Solvents
keywords: HSCCC; anti-proliferation; diarylheptanoid