title: Selenium-Containing Phycocyanin from Se-Enriched Spirulina platensis Reduces Inflammation in Dextran Sulfate Sodium-Induced Colitis by Inhibiting NF-κB Activation.
abstract: Selenium (Se) plays an important role in fine-tuning immune responses. Inflammatory bowel disease (IBD) involves hyperresponsive immunity of the digestive tract, and a low Se level might aggravate IBD progression; however, the beneficial effects of natural Se-enriched diets on IBD remain unknown. Previously, we developed high-yield Se-enriched Spirulina platensis (Se-SP) as an excellent organic nutritional Se source. Here we prepared Se-containing phycocyanin (Se-PC) from Se-SP and observed that Se-PC administration effectively reduced the extent of colitis in mouse induced by dextran sulfate sodium. Supplementation with Se-PC resulted in significant protective effects, including mitigation of body weight loss, bloody diarrhea, and colonic inflammatory damage. The anti-inflammatory effects of Se-PC supplementation were found to involve modulation of cytokines, including IL-6, TNF-α, MCP-1, and IL-10. Mechanistically, Se-PC inhibited the activation of macrophages by suppressing the nuclear translocation of NF-κB, which is involved in the transcription of these pro-inflammatory cytokines. These results together suggest potential benefits of Se-PC as a functional Se supplement to reduce the symptoms of IBD.
authors.affiliation: College of Pharmacy, Jinan University , Guangzhou 510632, Guangdong Province, China.
authors.forename: Chenghui
authors.initials: C
authors.lastname: Zhu
authors.affiliation: Department of Biotechnology, School of Life Science and Technology, Jinan University , Guangzhou 510632, Guangdong Province, China.
authors.forename: Qinjie
authors.initials: Q
authors.lastname: Ling
authors.affiliation: Department of Biotechnology, School of Life Science and Technology, Jinan University , Guangzhou 510632, Guangdong Province, China.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Cai
authors.affiliation: Department of Biotechnology, School of Life Science and Technology, Jinan University , Guangzhou 510632, Guangdong Province, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Biotechnology, School of Life Science and Technology, Jinan University , Guangzhou 510632, Guangdong Province, China.
authors.forename: Yibo
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Biotechnology, School of Life Science and Technology, Jinan University , Guangzhou 510632, Guangdong Province, China.|Department of Cell and Molecular Biology, John A. Burns School of Medicine, University of Hawaii , Honolulu, Hawaii 96813, United States.
authors.forename: Peter R
authors.initials: PR
authors.lastname: Hoffmann
authors.affiliation: Department of Chemistry, School of Life Science and Technology, Jinan University , Guangzhou 510632, Guangdong Province, China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Zheng
authors.affiliation: Department of Biotechnology, School of Life Science and Technology, Jinan University , Guangzhou 510632, Guangdong Province, China.
authors.forename: Tianhong
authors.initials: T
authors.lastname: Zhou
authors.affiliation: Department of Biotechnology, School of Life Science and Technology, Jinan University , Guangzhou 510632, Guangdong Province, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Huang
mesh_terms: D000818:Animals; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology; D016264:Dextran Sulfate / Q000009:adverse effects; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics / Q000276:immunology; D015850:Interleukin-6 / Q000235:genetics / Q000276:immunology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000235:genetics / Q000276:immunology; D010798:Phycocyanin / Q000008:administration & dosage* / Q000032:analysis; D012643:Selenium / Q000032:analysis*; D053545:Spirulina / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology
chemical_list: D015850:Interleukin-6; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D010798:Phycocyanin; D016753:Interleukin-10; D016264:Dextran Sulfate; D012643:Selenium
keywords: IBD; NF-κB pathway; Spirulina platensis; phycocyanin; selenium