title: A Highly Photostable Hyperbranched Polyglycerol-Based NIR Fluorescence Nanoplatform for Mitochondria-Specific Cell Imaging.
abstract: Considering the critical role of mitochondria in the life and death of cells, non-invasive long-term tracking of mitochondria has attracted considerable interest. However, a high-performance mitochondria-specific labeling probe with high photostability is still lacking. Herein a highly photostable hyperbranched polyglycerol (hPG)-based near-infrared (NIR) quantum dots (QDs) nanoplatform is reported for mitochondria-specific cell imaging. Comprising NIR Zn-Cu-In-S/ZnS QDs as extremely photostable fluorescent labels and alkyl chain (C12 )/triphenylphosphonium (TPP)-functionalized hPG derivatives as protective shell, the tailored QDs@hPG-C12 /TPP nanoprobe with a hydrodynamic diameter of about 65 nm exhibits NIR fluorescence, excellent biocompatibility, good stability, and mitochondria-targeted ability. Cell uptake experiments demonstrate that QDs@hPG-C12 /TPP displays a significantly enhanced uptake in HeLa cells compared to nontargeted QDs@hPG-C12 . Further co-localization study indicates that the probe selectively targets mitochondria. Importantly, compared with commercial deep-red mitochondria dyes, QDs@hPG-C12 /TPP possesses superior photostability under continuous laser irradiation, indicating great potential for long-term mitochondria labeling and tracking. Moreover, drug-loaded QDs@hPG-C12 /TPP display an enhanced tumor cell killing efficacy compared to nontargeted drugs. This work could open the door to the construction of organelle-targeted multifunctional nanoplatforms for precise diagnosis and high-efficient tumor therapy.
authors.affiliation: School of Life Sciences, School of Materials Science and Engineering, Tianjin Engineering Center of Micro-Nano Biomaterials and Detection-Treatment Technology, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin University, Tianjin, 300072, P. R. China.
authors.forename: Chunhong
authors.initials: C
authors.lastname: Dong
authors.affiliation: Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Research Center for Coastal Environmental Engineering and Technology of Shandong Province, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, Shandong, 264003, P. R. China.
authors.forename: Zhongyun
authors.initials: Z
authors.lastname: Liu
authors.affiliation: School of Life Sciences, School of Materials Science and Engineering, Tianjin Engineering Center of Micro-Nano Biomaterials and Detection-Treatment Technology, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin University, Tianjin, 300072, P. R. China.
authors.forename: Junqing
authors.initials: J
authors.lastname: Liu
authors.affiliation: Institut of Chemie and Biochemie, Freie Universität Berlin, Takustr. 3, 14195, Berlin, Germany.
authors.forename: Changzhu
authors.initials: C
authors.lastname: Wu
authors.affiliation: Institut of Chemie and Biochemie, Freie Universität Berlin, Takustr. 3, 14195, Berlin, Germany.
authors.forename: Falko
authors.initials: F
authors.lastname: Neumann
authors.affiliation: School of Life Sciences, School of Materials Science and Engineering, Tianjin Engineering Center of Micro-Nano Biomaterials and Detection-Treatment Technology, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin University, Tianjin, 300072, P. R. China.
authors.forename: Hanjie
authors.initials: H
authors.lastname: Wang
authors.affiliation: Institut für Pharmazie, Freie Universität Berlin, Königin-Luise-Str. 2-4, 14195, Berlin, Germany.
authors.forename: Monika
authors.initials: M
authors.lastname: Schäfer-Korting
authors.affiliation: Institut für Ernährungswissenschaft, Universität Potsdam, Arthur-Scheunert-Allee 114-116, 14558, Nuthetal, Germany.
authors.forename: Burkhard
authors.initials: B
authors.lastname: Kleuser
authors.affiliation: School of Life Sciences, School of Materials Science and Engineering, Tianjin Engineering Center of Micro-Nano Biomaterials and Detection-Treatment Technology, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin University, Tianjin, 300072, P. R. China. jinchang@tju.edu.cn.
authors.forename: Jin
authors.initials: J
authors.lastname: Chang
authors.affiliation: Reference and Translation Center for Cardiac Stem Cell Therapy, Universität Rostock, Schillingallee 69, 18057, Rostock, Germany.
authors.forename: Wenzhong
authors.initials: W
authors.lastname: Li
authors.affiliation: Institut of Chemie and Biochemie, Freie Universität Berlin, Takustr. 3, 14195, Berlin, Germany. nan.ma@hzg.de.|Institute of Biomaterial Research, Helmholtz-Zentrum Geesthacht, Kantstraße 55, 14513, Teltow-Seehof, Germany. nan.ma@hzg.de.
authors.forename: Nan
authors.initials: N
authors.lastname: Ma
authors.affiliation: Institut of Chemie and Biochemie, Freie Universität Berlin, Takustr. 3, 14195, Berlin, Germany. haag@chemie.fu-berlin.de.
authors.forename: Rainer
authors.initials: R
authors.lastname: Haag
mesh_terms: D016503:Drug Delivery Systems / Q000379:methods*; D005990:Glycerol / Q000737:chemistry / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods; D008928:Mitochondria / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology; D045663:Quantum Dots / Q000737:chemistry*
chemical_list: D011108:Polymers; C043941:polyglycerol; D005990:Glycerol
keywords: NIR ZCIS/ZnS QDs; hyperbranched polyglycerol; mitochondria targeting; photostability; tumor theranostics