title: Purification, Characterization and Biological Activity of Polysaccharides from Dendrobium officinale.
abstract: Polysaccharide (DOPA) from the stem of D. officinale, as well as two fractions (DOPA-1 and DOPA-2) of it, were isolated and purified by DEAE cellulose-52 and Sephacryl S-300 chromatography, and their structural characteristics and bioactivities were investigated. The average molecular weights of DOPA-1 and DOPA-2 were 394 kDa and 362 kDa, respectively. They were mainly composed of d-mannose, d-glucose, and had a backbone consisting of 1,4-linked β-d-Manp and 1,4-linked β-d-Glcp with O-acetyl groups. Bioactivity studies indicated that both DOPA and its purified fractions (DOPA-1 and DOPA-2) could activate splenocytes and macrophages. The D. officinale polysaccharides had stimulatory effects on splenocytes, T-lymphocytes and B-lymphocytes, promoting the cell viability and NO production of RAW 264.7 macrophages. Furthermore, DOPA, DOPA-1 and DOPA-2 were found to protect RAW 264.7 macrophages against hydrogen peroxide (H₂O₂)-induced oxidative injury by promoting cell viability, suppressing apoptosis and ameliorating oxidative lesions. These results suggested that D. officinale polysaccharides possessed antioxidant activity and mild immunostimulatory activity.
authors.affiliation: College of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China. huangkaiweifeng@163.com.
authors.forename: Kaiwei
authors.initials: K
authors.lastname: Huang
authors.affiliation: College of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China. YYLi0711025@163.com.
authors.forename: Yunrong
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China. Taoshengchang2013@163.com.
authors.forename: Shengchang
authors.initials: S
authors.lastname: Tao
authors.affiliation: College of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China. weigang021@163.com.
authors.forename: Gang
authors.initials: G
authors.lastname: Wei
authors.affiliation: The First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou 510080, China. huangyuechun@163.com.
authors.forename: Yuechun
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Anatomy, Guangzhou University of Chinese Medicine, Guangzhou 510006, China. Cdf27212@21cn.com.
authors.forename: Dongfeng
authors.initials: D
authors.lastname: Chen
authors.affiliation: College of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China. W18719138511@hotmail.com.
authors.forename: Chengfeng
authors.initials: C
authors.lastname: Wu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002850:Chromatography, Gel; D031667:Dendrobium / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007155:Immunologic Factors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008745:Methylation; D051379:Mice; D008970:Molecular Weight; D009569:Nitric Oxide / Q000096:biosynthesis; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000975:Antioxidants; D007155:Immunologic Factors; D010936:Plant Extracts; D011134:Polysaccharides; D009569:Nitric Oxide; D006861:Hydrogen Peroxide
keywords: Dendrobium officinale; antioxidant activity; immunostimulatory activity; polysaccharide; structural characterization