title: Fertility and flow cytometry study of frozen-thawed sperm in cryopreservation medium supplemented with soybean lecithin.
abstract: Semen cryopreservation can provide genetic resources for a large number of females from a small number of superior males. Optimization of cryopreservation media to achieve the highest quality of post-thaw semen is crucial. Soybean lecithin has evaluated as a plant-based cryoprotectant for substitution of egg yolk in ram semen extender. Flow cytometric and fertility assessments were applied following cryopreservation procedure in two experimental groups (SL group: extender containing 1% w/v soybean lecithin and EY group: extender containing 20% v/v egg yolk). The higher percentage of live sperm and the lower percentage of dead sperm were obtained in SL (47.66 ± 1.38, 52.33 ± 1.69, respectively) extender compared to EY (41.16 ± 1.38, 58.83 ± 1.69). For motion characteristics, plasma membrane integrity, acrosome integrity and mitochondria activity, no significant difference was observed between SL and EY extenders. In artificial insemination experiment, there was no significant difference in pregnancy rate, lambing rate and twining rate between SL and EY extenders. It can be concluded that SL extender can be an efficient alternative extender to preserve ram sperm during cryopreservation procedure without adverse effects.
authors.affiliation: Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran. Electronic address: rezamasoudi@ut.ac.ir.
authors.forename: R
authors.initials: R
authors.lastname: Masoudi
authors.affiliation: Department of Poultry Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran. Electronic address: m.sharafi@modares.ac.ir.
authors.forename: M
authors.initials: M
authors.lastname: Sharafi
authors.affiliation: Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Zareh Shahneh
authors.affiliation: Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Towhidi
authors.affiliation: Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
authors.forename: H
authors.initials: H
authors.lastname: Kohram
authors.affiliation: Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
authors.forename: V
authors.initials: V
authors.lastname: Esmaeili
authors.affiliation: Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Shahverdi
authors.affiliation: Department of Research, Breeding and Production of Laboratory Animals, Rzai Vaccine and Serum Research Institute, Karaj, Iran.
authors.forename: N Dadashpour
authors.initials: ND
authors.lastname: Davachi
mesh_terms: D000818:Animals; D002462:Cell Membrane; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004530:Egg Yolk; D005260:Female; D005298:Fertility / Q000187:drug effects*; D005434:Flow Cytometry; D007315:Insemination, Artificial; D054709:Lecithins / Q000494:pharmacology*; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology; D011247:Pregnancy; D012661:Semen / Q000187:drug effects; D012662:Semen Preservation / Q000379:methods*; D012756:Sheep; D013025:Glycine max; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects
chemical_list: D003451:Cryoprotective Agents; D054709:Lecithins; D010936:Plant Extracts
keywords: Artificial insemination; Cryopreservation; Flow cytometry; Soybean lecithin