title: Complete genome sequence of a coxsackievirus B3 recombinant isolated from an aseptic meningitis outbreak in eastern China.
abstract: Coxsackievirus B3 (CV-B3) has frequently been associated with aseptic meningitis outbreaks in China. To identify sequence motifs related to aseptic meningitis and to construct an infectious clone, the genome sequence of 08TC170, a representative strain isolated from cerebrospinal fluid (CSF) samples from an outbreak in Shandong in 2008, was determined, and the coding regions for P1-P3 and VP1 were aligned. The first 21 and last 20 residues were "TTAAAACAGCCTGTGGGTTGT" and "ATTCTCCGCATTCGGTGCGG", respectively. The whole genome consisted of 7401 nucleotides, sharing 80.8 % identity with the prototype strain Nancy and low sequence similarity with members of clusters A-C. In contrast, 08TC170 showed high sequence similarity to members of cluster D. An especially high level of sequence identity (≥97.7 %) was found within a branch constituted by 08TC170 and four Chinese strains that clustered together in all of the P1-P3 phylogenic trees. In addition, 08TC170 also possessed a close relationship to the Hong Kong strain 26362/08 in VP1. Similarity plot analysis showed that 08TC170 was most similar to the Chinese CV-B3 strain SSM in P1 and the partial P2 coding region but to the CV-B5 or E-6 strain in 2C and following regions. A T277A mutation was found in 08TC170 and other strains isolated in 2008-2010, but not in strains isolated before 2008, which had high sequence similarity and formed the cluster A277. The results suggested that 08TC170 was the product of both intertypic recombination and point mutation, whose effects on viral neurovirulence will be investigated in a further study. The high homology between 08TC170 and other strains revealed their co-circulation in mainland China and Hong Kong and indicates that further surveillance is needed.
authors.affiliation: Academy of Preventive Medicine, Shandong University, Jinan, People's Republic of China.|Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, Shandong Center for Disease Control and Prevention, Jinan, People's Republic of China.
authors.forename: Wenqiang
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Academy of Preventive Medicine, Shandong University, Jinan, People's Republic of China.|Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, Shandong Center for Disease Control and Prevention, Jinan, People's Republic of China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Lin
authors.affiliation: Department of Molecular Genetics and Microbiology, School of Medicine, Stony Brook University, Stony Brook, NY, USA.
authors.forename: Ping
authors.initials: P
authors.lastname: Jiang
authors.affiliation: Academy of Preventive Medicine, Shandong University, Jinan, People's Republic of China.|Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, Shandong Center for Disease Control and Prevention, Jinan, People's Republic of China.
authors.forename: Zexin
authors.initials: Z
authors.lastname: Tao
authors.affiliation: Academy of Preventive Medicine, Shandong University, Jinan, People's Republic of China.|Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, Shandong Center for Disease Control and Prevention, Jinan, People's Republic of China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Academy of Preventive Medicine, Shandong University, Jinan, People's Republic of China.|Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, Shandong Center for Disease Control and Prevention, Jinan, People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Ji
authors.affiliation: Academy of Preventive Medicine, Shandong University, Jinan, People's Republic of China.|Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, Shandong Center for Disease Control and Prevention, Jinan, People's Republic of China.
authors.forename: Tongzhan
authors.initials: T
authors.lastname: Wang
authors.affiliation: Academy of Preventive Medicine, Shandong University, Jinan, People's Republic of China.|Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, Shandong Center for Disease Control and Prevention, Jinan, People's Republic of China.
authors.forename: Suting
authors.initials: S
authors.lastname: Wang
authors.affiliation: Academy of Preventive Medicine, Shandong University, Jinan, People's Republic of China.|Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, Shandong Center for Disease Control and Prevention, Jinan, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Lv
authors.affiliation: Academy of Preventive Medicine, Shandong University, Jinan, People's Republic of China.|Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, Shandong Center for Disease Control and Prevention, Jinan, People's Republic of China.
authors.forename: Aiqiang
authors.initials: A
authors.lastname: Xu
authors.affiliation: Academy of Preventive Medicine, Shandong University, Jinan, People's Republic of China. whyan732002@163.com.|Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, Shandong Center for Disease Control and Prevention, Jinan, People's Republic of China. whyan732002@163.com.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Wang
mesh_terms: D001483:Base Sequence; D002681:China / Q000453:epidemiology; D004196:Disease Outbreaks; D029822:Enterovirus B, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D016679:Genome, Viral; D023281:Genomics; D005838:Genotype; D006801:Humans; D008582:Meningitis, Aseptic / Q000453:epidemiology / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011995:Recombination, Genetic; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins