title: Repair of dentin defects from DSPP knockout mice by PILP mineralization.
abstract: Dentinogenesis imperfecta type II (DGI-II) lacks intrafibrillar mineral with severe compromise of dentin mechanical properties. ADsppknockout (Dspp-/-) mouse, with a phenotype similar to that of human DGI-II, was used to determine if poly-L-aspartic acid [poly(ASP)] in the "polymer-induced liquid-precursor" (PILP) system can restore its mechanical properties. Dentin from six-week oldDspp-/-and wild-type mice was treated with CaP solution containing poly(ASP) for up to 14 days. Elastic modulus and hardness before and after treatment were correlated with mineralization from Micro x-ray computed tomography (Micro-XCT). Transmission electron microscopy (TEM)/Selected area electron diffraction (SAED) were used to compare matrix mineralization and crystallography. Mechanical properties of theDspp-/-dentin were significantly less than wild-type dentin and recovered significantly (P< 0.05) after PILP-treatment, reaching values comparable to wild-type dentin. Micro-XCT showed mineral recovery similar to wild-type dentin after PILP-treatment. TEM/SAED showed repair of patchy mineralization and complete mineralization of defective dentin. This approach may lead to new strategies for hard tissue repair.
authors.affiliation: Department of Preventive and Restorative Dental Sciences, University of California, San Francisco, San Francisco, California 94143, USA.
authors.forename: H
authors.initials: H
authors.lastname: Nurrohman
authors.affiliation: Department of Preventive and Restorative Dental Sciences, University of California, San Francisco, San Francisco, California 94143, USA.
authors.forename: K
authors.initials: K
authors.lastname: Saeki
authors.affiliation: Department of Preventive and Restorative Dental Sciences, University of California, San Francisco, San Francisco, California 94143, USA.
authors.forename: K
authors.initials: K
authors.lastname: Carneiro
authors.affiliation: Department of Preventive and Restorative Dental Sciences, University of California, San Francisco, San Francisco, California 94143, USA.
authors.forename: Y C
authors.initials: YC
authors.lastname: Chien
authors.affiliation: Department of Preventive and Restorative Dental Sciences, University of California, San Francisco, San Francisco, California 94143, USA.
authors.forename: S
authors.initials: S
authors.lastname: Djomehri
authors.affiliation: Department of Preventive and Restorative Dental Sciences, University of California, San Francisco, San Francisco, California 94143, USA.
authors.forename: S P
authors.initials: SP
authors.lastname: Ho
authors.affiliation: Department of Biomedical Sciences and Center for Craniofacial Research and Diagnosis, Texas A&M University Baylor College of Dentistry, Dallas, Texas 75246, USA.
authors.forename: C
authors.initials: C
authors.lastname: Qin
authors.affiliation: Department of Preventive and Restorative Dental Sciences, University of California, San Francisco, San Francisco, California 94143, USA.
authors.forename: S J
authors.initials: SJ
authors.lastname: Marshall
authors.affiliation: Department of Materials Science & Engineering, University of Florida, Gainesville, Florida 32611, USA.
authors.forename: L B
authors.initials: LB
authors.lastname: Gower
authors.affiliation: Department of Preventive and Restorative Dental Sciences, University of California, San Francisco, San Francisco, California 94143, USA.
authors.forename: G W
authors.initials: GW
authors.lastname: Marshall
authors.affiliation: Department of Preventive and Restorative Dental Sciences, University of California, San Francisco, San Francisco, California 94143, USA.
authors.forename: S
authors.initials: S
authors.lastname: Habelitz