title: Pathological and histometric analysis of the gills of female Hyphessobrycon eques (Teleostei:Characidae) exposed to different concentrations of the insecticide Dimilin(®).
abstract: Female individuals of Hyphessobrycon eques were exposed to Diflubenzuron (Dimilin(®)) in order to determine whether exposure to sublethal levels of this insecticide causes changes in gill morphology. Fish were exposed to 0.01, 0.1 and 1.0mgL(-1) for 96h and 17 days and then submitted to pathological and histometric evaluation. Pathological lesions, such as hyperplasia, lamellar fusion, vascular congestion, secondary lamellar disarray, vasodilatation, hemorrhage and increased lamellar epithelium, were significantly more common in the gills of fish exposed to Dimilin(®) than the control. Histometric analysis documented significant changes in blood vessel diameter, primary lamellae width and secondary lamellae length, and the appearance of hemorrhage foci in all concentrations tested. Even at low Dimilin(®) concentrations, the histopathological alteration index was mild to moderate, thereby indicating that the function of this tissue was compromised. These findings indicate that indiscriminate use of Dimilin(®) can adversely affect the structural integrity of the gills of H. eques, which can cause numerous problems for fish farming systems.
authors.affiliation: Programa de Pós-Graduacão em Zoologia de Vertebrados da PUC Minas, Avenue Dom José Gaspar, 500, Coração Eucarístico, Belo Horizonte, Minas Gerais, Brasil. Electronic address: kalukasss@hotmail.com.
authors.forename: Lucas
authors.initials: L
authors.lastname: Marcon
authors.affiliation: Departamento de Veterinária, Universidade Federal de Viçosa, Avenue Peter Henry Rolfs S/N, Campus Universitário, Viçosa, Minas Gerais, Brasil.
authors.forename: Diego Senra
authors.initials: DS
authors.lastname: Lopes
authors.affiliation: Departamento de Engenharia Civil, Universidade Federal de Viçosa, Avenue Peter Henry Rolfs S/N, Campus Universitário, Viçosa, Minas Gerais, Brasil.
authors.forename: Ann Honor
authors.initials: AH
authors.lastname: Mounteer
authors.affiliation: Departamento de Veterinária, Universidade Federal de Viçosa, Avenue Peter Henry Rolfs S/N, Campus Universitário, Viçosa, Minas Gerais, Brasil.
authors.forename: Amara Manarino Andrade
authors.initials: AM
authors.lastname: Goulart
authors.affiliation: Departamento de Veterinária, Universidade Federal de Viçosa, Avenue Peter Henry Rolfs S/N, Campus Universitário, Viçosa, Minas Gerais, Brasil.
authors.forename: Mila Vasques
authors.initials: MV
authors.lastname: Leandro
authors.affiliation: Departamento de Veterinária, Universidade Federal de Viçosa, Avenue Peter Henry Rolfs S/N, Campus Universitário, Viçosa, Minas Gerais, Brasil. Electronic address: laerciob@hotmail.com.
authors.forename: Laércio
authors.initials: L
authors.lastname: Dos Anjos Benjamin
mesh_terms: D000818:Animals; D060126:Characidae; D004132:Diflubenzuron / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005880:Gills / Q000187:drug effects* / Q000473:pathology; D007306:Insecticides / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D007306:Insecticides; D014874:Water Pollutants, Chemical; D004132:Diflubenzuron
keywords: Biomarkers; Environment; Histology; Teleost fish; Toxicity