title: Start-up and microbial communities of a simultaneous nitrogen removal system for high salinity and high nitrogen organic wastewater via heterotrophic nitrification.
abstract: In this study, a simultaneous nitrogen removal system for high salinity and high nitrogen organic wastewater was developed in a pressurized biofilm reactor. The result showed that under the air supply rate of 200Lh(-1), salinity of 3.0±0.2%, organic load of 10kgCODm(-3)d(-1) and nitrogen loading of 0.185kgm(-3)d(-1), the reactor started up rapidly and performed stably after 30days operation. Meanwhile, a simultaneous COD and nitrogen removal was achieved in the single-stage reactor, with COD, NH4(+)-N and TN removal efficiency of 97%, 99% and 98%, respectively. Denaturing gradient gel electrophoresis profile demonstrated that simultaneous nitrogen removal could be achieved through heterotrophic nitrification-aerobic denitrification, and the pivotal microorganisms were Flavobacterium phragmitis and Paracoccus denitrificans. The microbial community of salt-tolerant halophilic microorganisms was developed successfully. This study can provide a more efficient and feasible solution to treat high salinity organic wastewater.
authors.affiliation: Faculty of Urban Construction and Environmental Engineering, Chongqing University, Chongqing 400045, PR China; Key Laboratory of the Three Gorges Reservoir's Eco-Environments, Ministry of Education, Chongqing University, Chongqing 400045, PR China.
authors.forename: Jiahao
authors.initials: J
authors.lastname: Chen
authors.affiliation: Faculty of Urban Construction and Environmental Engineering, Chongqing University, Chongqing 400045, PR China; School of Environmental and Municipal Engineering, Tianjin Chengjian University, Tianjin 300384, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Han
authors.affiliation: Faculty of Urban Construction and Environmental Engineering, Chongqing University, Chongqing 400045, PR China; Key Laboratory of the Three Gorges Reservoir's Eco-Environments, Ministry of Education, Chongqing University, Chongqing 400045, PR China.
authors.forename: Yingmu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Faculty of Urban Construction and Environmental Engineering, Chongqing University, Chongqing 400045, PR China; Key Laboratory of the Three Gorges Reservoir's Eco-Environments, Ministry of Education, Chongqing University, Chongqing 400045, PR China.
authors.forename: Benzhou
authors.initials: B
authors.lastname: Gong
authors.affiliation: Faculty of Urban Construction and Environmental Engineering, Chongqing University, Chongqing 400045, PR China; Key Laboratory of the Three Gorges Reservoir's Eco-Environments, Ministry of Education, Chongqing University, Chongqing 400045, PR China. Electronic address: dkmotes@163.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Faculty of Urban Construction and Environmental Engineering, Chongqing University, Chongqing 400045, PR China; Key Laboratory of the Three Gorges Reservoir's Eco-Environments, Ministry of Education, Chongqing University, Chongqing 400045, PR China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Qing
mesh_terms: D000641:Ammonia / Q000378:metabolism; D018441:Biofilms; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology; D058645:Denaturing Gradient Gel Electrophoresis; D058440:Denitrification; D005417:Flavobacterium / Q000502:physiology; D052836:Heterotrophic Processes; D059013:Microbial Consortia / Q000502:physiology*; D058465:Nitrification; D009584:Nitrogen / Q000302:isolation & purification / Q000378:metabolism; D010231:Paracoccus denitrificans / Q000502:physiology; D054712:Salinity; D014865:Waste Disposal, Fluid / Q000379:methods*; D062065:Wastewater / Q000737:chemistry*
chemical_list: D000641:Ammonia; D009584:Nitrogen; D062065:Wastewater
keywords: Aerobic denitrification; Bacterial community; Heterotrophic nitrification; High salinity wastewater; Pressurized biofilm reactor