title: Molasses melanoidin promotes copper uptake for radish sprouts: the potential for an accelerator of phytoextraction.
abstract: Phytoextraction has been proposed as an alternative remediation technology for heavy metal contamination, and it is well known that chelators may alter the toxicity of heavy metals and the bioavailability in plants. Our previous work demonstrated that an adsorbent-column chromatography can effectively separate melanoidin-like product (MLP) from sugarcane molasses. The aim of this study was to examine the chelating property of MLP and to evaluate the facilitatory influence on the phytoextraction efficiency of Japanese radish. The result showed that MLP binds to all the metal ions examined and the binding capacity of MLP toward Cu(2+) seems to be the highest among them. The metal detoxification by MLP followed the order of Pb(2+) > Zn(2+) > Ni(2+) > Cu(2+) > Fe(2+) > Cd(2+) > Co(2+). Furthermore, in the phytoextraction experiment using copper sulfate, the application of MLP accelerated the detoxification of copper and the bioavailability in radish sprouts. Thus, these results suggest that MLP possesses the potential for an accelerator of phytoextraction in the copper-contaminated media.
authors.affiliation: Division of Molecular Science, Faculty of Science and Technology, Gunma University, 1-5-1 Tenjin-cho, Kiryu, Gunma, 376-8515, Japan. hatano@gunma-u.ac.jp.
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Hatano
authors.affiliation: Division of Molecular Science, Faculty of Science and Technology, Gunma University, 1-5-1 Tenjin-cho, Kiryu, Gunma, 376-8515, Japan.
authors.forename: Kazuki
authors.initials: K
authors.lastname: Kanazawa
authors.affiliation: Division of Molecular Science, Faculty of Science and Technology, Gunma University, 1-5-1 Tenjin-cho, Kiryu, Gunma, 376-8515, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Tomura
authors.affiliation: Division of Molecular Science, Faculty of Science and Technology, Gunma University, 1-5-1 Tenjin-cho, Kiryu, Gunma, 376-8515, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yamatsu
authors.affiliation: Division of Molecular Science, Faculty of Science and Technology, Gunma University, 1-5-1 Tenjin-cho, Kiryu, Gunma, 376-8515, Japan.
authors.forename: Kin-Ichi
authors.initials: K
authors.lastname: Tsunoda
authors.affiliation: Division of Molecular Science, Faculty of Science and Technology, Gunma University, 1-5-1 Tenjin-cho, Kiryu, Gunma, 376-8515, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Kubota
mesh_terms: D001673:Biodegradation, Environmental; D001682:Biological Availability; D002614:Chelating Agents / Q000737:chemistry; D003300:Copper / Q000032:analysis / Q000378:metabolism*; D052918:Environmental Restoration and Remediation / Q000379:methods; D008965:Molasses; D010944:Plants / Q000378:metabolism; D011108:Polymers / Q000737:chemistry*; D031224:Raphanus / Q000378:metabolism*; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism*
chemical_list: D002614:Chelating Agents; D011108:Polymers; D012989:Soil Pollutants; C011908:melanoidin polymers; D003300:Copper
keywords: Heavy metal; Japanese radish; Melanoidin; Metal binding capacity; Metal detoxification; Phytoextraction; Sugarcane molasses