title: Proteomic analysis of minute amount of colonic biopsies by enteroscopy sampling.
abstract: Colorectal cancer (CRC) is one of the most common types of malignant tumor worldwide. Currently, although many researchers have been devoting themselves in CRC studies, the process of locating biomarkers for CRC early diagnosis and prognostic is still very slow. Using a centrifugal proteomic reactor-based proteomic analysis of minute amount of colonic biopsies by enteroscopy sampling, 2620 protein groups were quantified between cancer mucosa and adjacent normal colorectal mucosa. Of which, 403 protein groups were differentially expressed with statistic significance between cancer and normal tissues, including 195 up-regulated and 208 down-regulated proteins in cancer tissues. Three proteins (SOD3, PRELP and NGAL) were selected for further Western blot validation. And the resulting Western blot experimental results were consistent with the quantitative proteomic data. SOD3 and PRELP are down-regulated in CRC mucosa comparing to adjacent normal tissue, while NGAL is up-regulated in CRC mucosa. In conclusion, the centrifugal proteomic reactor-based label-free quantitative proteomic approach provides a highly sensitive and powerful tool for analyzing minute protein sample from tiny colorectal biopsies, which may facilitate CRC biomarkers discovery for diagnoses and prognoses.
authors.affiliation: Department of Analytical Chemistry and CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Liu
authors.affiliation: Fuyang People's Hospital, China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Analytical Chemistry and CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Meng
authors.affiliation: Digestive Endoscopic Center, Shanghai Jiaotong University Affiliated Sixth People's Hospital, China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Zheng
authors.affiliation: Department of Analytical Chemistry and CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, China.
authors.forename: Jianhong
authors.initials: J
authors.lastname: Wu
authors.affiliation: Shanghai Key Laboratory of Diabetes Mellitus, Department of Endocrinology and Metabolism, Shanghai Diabetes Institute, Shanghai Clinical Center for Diabetes, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.affiliation: Shanghai Key Laboratory of Diabetes Mellitus, Department of Endocrinology and Metabolism, Shanghai Diabetes Institute, Shanghai Clinical Center for Diabetes, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, China.
authors.forename: Weiping
authors.initials: W
authors.lastname: Jia
authors.affiliation: Department of Biochemistry, Microbiology and Immunology, and Department of Chemistry and Biomolecular Sciences, University of Ottawa, Canada.
authors.forename: Daniel
authors.initials: D
authors.lastname: Figeys
authors.affiliation: Digestive Endoscopic Center, Shanghai Jiaotong University Affiliated Sixth People's Hospital, China. Electronic address: emulan@163.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chang
authors.affiliation: Department of Analytical Chemistry and CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, China. Electronic address: zhouhu@simm.ac.cn.
authors.forename: Hu
authors.initials: H
authors.lastname: Zhou
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis / Q000378:metabolism; D003106:Colon / Q000378:metabolism / Q000473:pathology*; D015179:Colorectal Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D055088:Early Detection of Cancer; D004724:Endoscopy; D016326:Extracellular Matrix Proteins / Q000032:analysis / Q000378:metabolism; D006023:Glycoproteins / Q000032:analysis / Q000378:metabolism; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology*; D000071068:Lipocalin-2 / Q000032:analysis / Q000378:metabolism; D025941:Protein Interaction Mapping; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D012007:Rectum / Q000378:metabolism / Q000473:pathology*; D018401:Sample Size; D015398:Signal Transduction; D013482:Superoxide Dismutase / Q000032:analysis / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D016326:Extracellular Matrix Proteins; D006023:Glycoproteins; C489506:LCN2 protein, human; D000071068:Lipocalin-2; C096382:PRELP protein, human; D020543:Proteome; C441642:SOD3 protein, human; D013482:Superoxide Dismutase
keywords: Centrifugal proteomic reactor; Colorectal cancer; Endoscopy; Label-free proteomics; Minute amount