title: Acrylamide affects proliferation and differentiation of the neural progenitor cell line C17.2 and the neuroblastoma cell line SH-SY5Y.
abstract: Acrylamide is a well-known neurotoxic compound and people get exposed to the compound by food consumption and environmental pollutants. Since acrylamide crosses the placenta barrier, the fetus is also being exposed resulting in a risk for developmental neurotoxicity. In this study, the neural progenitor cell line C17.2 and the neuroblastoma cell line SH-SY5Y were used to study proliferation and differentiation as alerting indicators for developmental neurotoxicity. For both cell lines, acrylamide reduced the number of viable cells by reducing proliferation and inducing cell death in undifferentiated cells. Acrylamide concentrations starting at 10fM attenuated the differentiation process in SH-SY5Y cells by sustaining cell proliferation and neurite outgrowth was reduced at concentrations from 10pM. Acrylamide significantly reduced the number of neurons starting at 1μM and altered the ratio between the different phenotypes in differentiating C17.2 cell cultures. Ten micromolar of acrylamide also reduced the expression of the neuronal and astrocyte biomarkers. Although the neurotoxic concentrations in the femtomolar range seem to be specific for the SH-SY5Y cell line, the fact that micromolar concentrations of acrylamide seem to attenuate the differentiation process in both cell lines raises the interest to further investigations on the possible developmental neurotoxicity of acrylamide.
authors.affiliation: Department of Neurochemistry, Stockholm University, Stockholm 106 91, Sweden. Electronic address: kristina.attoff@neurochem.su.se.
authors.forename: K
authors.initials: K
authors.lastname: Attoff
authors.affiliation: Department of Neurochemistry, Stockholm University, Stockholm 106 91, Sweden. Electronic address: dike5562@student.su.se.
authors.forename: D
authors.initials: D
authors.lastname: Kertika
authors.affiliation: Department of Neurochemistry, Stockholm University, Stockholm 106 91, Sweden. Electronic address: jessica.lundqvist@neurochem.su.se.
authors.forename: J
authors.initials: J
authors.lastname: Lundqvist
authors.affiliation: Department of Biology, Lund University, Lund 223 62, Sweden. Electronic address: stina.oredsson@biol.lu.se.
authors.forename: S
authors.initials: S
authors.lastname: Oredsson
authors.affiliation: Department of Neurochemistry, Stockholm University, Stockholm 106 91, Sweden; Swedish Toxicology Science Center (Swetox), Södertälje, Sweden. Electronic address: anna.forsby@neurochem.su.se.
authors.forename: A
authors.initials: A
authors.lastname: Forsby
mesh_terms: D020106:Acrylamide / Q000633:toxicity*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D051379:Mice; D058953:Neural Stem Cells / Q000187:drug effects; D009447:Neuroblastoma
chemical_list: D020106:Acrylamide
keywords: Acrylamide; C17.2; Developmental neurotoxicity; Differentiation; Neural progenitor cells; SH-SY5Y