title: Fibronectin adsorption on surface-modified polyetherurethanes and their differentiated effect on specific blood elements related to inflammatory and clotting processes.
abstract: After the introduction of a medical device into the body, adhesive proteins such as fibronectin (Fn) will adsorb to the surface of the biomaterial. Monocytes (MCs) will interact with these adsorbed proteins, and adopt either a proinflammatory and/or prowound healing phenotype, thereby influencing many blood interaction events including thrombogenesis. In this work, Fn adsorption as well as subsequent MC response and thrombus formation were investigated on two surfaces-modified polyetherurethanes (PEUs) using different surface modifiers: an anionic/dihydroxyl oligomeric (ADO) additive, known to enable cell adhesion, and a fluorinated polypropylene oxide oligomer (PPO), known to reduce platelet adhesion. Results indicated that at 24 h of MC culture, PEU-ADO and PEU-PPO promoted an anti-inflammatory character relative to the base PEU. Longer clotting times, based on a free hemoglobin assay, were also found on the two surface-modified PEUs relative to the native one, suggesting their potential for the reduction of thrombus formation. In presence of a Fn monolayer, the surface-modified PEUs conserved a lower thrombogenic character than the base PEU, and was however significantly decreased when compared to prior protein adsorption. Furthermore, Fn coatings increased the MC production levels of tumor necrosis factor-α and interleukin-10 at 24 h, while not affecting the anti-inflammatory effect of the modifications relative to the base PEU. This finding was most prominent on PEU-PPO, suggesting that the interaction of the adsorbed Fn with blood cells was different for the two additives. Hence, the results highlighted differentiating effects of Fn adsorption on specific blood activating processes related to inflammatory and thrombotic responses.
authors.affiliation: Laboratory for Biomaterials and Bioengineering, CRC-I, Department of Min-Met-Mater Engineering, Laval University and Research Center of CHU de Québec, Québec city, Québec G1A 0V6, Canada; Laboratoire ERRMECe, Université de Cergy-Pontoise, 2 Avenue Adolphe Chauvin, 95302 Cergy-Pontoise Cedex, France; and Faculty of Dentistry, University of Toronto, Toronto, Ontario M5G 1G6, Canada.
authors.forename: Ludivine
authors.initials: L
authors.lastname: Hugoni
authors.affiliation: Laboratory for Biomaterials and Bioengineering, CRC-I, Department of Min-Met-Mater Engineering, Laval University and Research Center of CHU de Québec, Québec city, Québec G1A 0V6, Canada and Laboratoire ERRMECe, Université de Cergy-Pontoise, 2 Avenue Adolphe Chauvin, 95302 Cergy-Pontoise Cedex, France.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Montaño-Machado
authors.affiliation: Faculty of Dentistry, University of Toronto, Toronto, Ontario M5G 1G6, Canada.
authors.forename: Meilin
authors.initials: M
authors.lastname: Yang
authors.affiliation: Laboratoire ERRMECe, Université de Cergy-Pontoise, 2 Avenue Adolphe Chauvin, 95302 Cergy-Pontoise Cedex, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Pauthe
authors.affiliation: Laboratory for Biomaterials and Bioengineering, CRC-I, Department of Min-Met-Mater Engineering, Laval University and Research Center of CHU de Québec, Québec city, Québec G1A 0V6, Canada.
authors.forename: Diego
authors.initials: D
authors.lastname: Mantovani
authors.affiliation: Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario M5S 3G9, Canada and Faculty of Dentistry, University of Toronto, Toronto, Ontario M5G 1G6, Canada.
authors.forename: J Paul
authors.initials: JP
authors.lastname: Santerre
mesh_terms: D000327:Adsorption; D020099:Coated Materials, Biocompatible; D005353:Fibronectins / Q000378:metabolism*; D064368:Healthy Volunteers; D006801:Humans; D007249:Inflammation / Q000139:chemically induced*; D009000:Monocytes / Q000502:physiology; D011140:Polyurethanes / Q000378:metabolism*; D013499:Surface Properties; D013927:Thrombosis / Q000139:chemically induced*
chemical_list: D020099:Coated Materials, Biocompatible; D005353:Fibronectins; D011140:Polyurethanes; C115016:polyetherurethane