title: Staining Fission Yeast Filamentous Actin with Fluorescent Phalloidin Conjugates.
abstract: The Schizosaccharomyces pombe filamentous (F)-actin cytoskeleton drives cell growth, morphogenesis, endocytosis, and cytokinesis. The protocol described here reveals the distribution of F-actin in fixed cells through the use of fluorescently conjugated phalloidin. Simultaneous staining of cell wall landmarks (with calcofluor) and chromatin (with 4',6-diamidino-2-phenylindole, or DAPI) makes this rapid staining procedure highly effective for staging cell cycle progression, monitoring morphogenetic abnormalities, and assessing the impact of environmental and genetic changes on the integrity of the F-actin cytoskeleton.
authors.affiliation: CRUK Cell Division Group, Cancer Research UK Manchester Institute, University of Manchester, Manchester M20 4BX, United Kingdom.
authors.forename: Iain M
authors.initials: IM
authors.lastname: Hagan
mesh_terms: D000199:Actins / Q000032:analysis*; D005456:Fluorescent Dyes / Q000378:metabolism*; D008856:Microscopy, Fluorescence / Q000379:methods*; D010590:Phalloidine / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000166:cytology*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D000199:Actins; D005456:Fluorescent Dyes; D010590:Phalloidine