Implications of oxidative stress in the brain plasticity originated by fasting: a BOLD-fMRI study.
OBJECTIVE
The goal of this study was assessing the intermittent fasting effect on brain plasticity and oxidative stress (OS) using blood-oxygenation-level dependent (BOLD)-functional magnetic resonance image (fMRI) approach. Evidences of physiological and molecular phenomena involved in this process are discussed and compared to reported literature.


METHODS
Six fully healthy male non-smokers volunteered in this study. All volunteers were right handed, and have an equilibrated, consistent and healthy daily nutritional habit, and a healthy lifestyle. Participants were allowed consuming food during evening and night time while fasting with self-prohibiting food and liquids during 14 hours/day from sunrise to sunset. All participants underwent identical brain BOLD-fMRI protocol. The images were acquired in the Department of Radiology and Clinical Imaging of the University Hospital of Fez, Fez, Morocco. The anatomical brain and BOLD-fMRIs were acquired using a 1.5-Tesla scanner (Signa, General Electric, Milwaukee, United States). BOLD-fMRI image acquisition was done using single-shot gradient echo echo-planer imaging sequence. BOLD-fMRI paradigm consisted of the motor task where volunteers were asked to perform finger taping of the right hand. Two BOLD-fMRI scan sessions were performed, the first one between the 5th and 10th days preceding the start of fasting and the second between days 25th and 28th of the fasting month. All sessions were performed between 3:30 PM and 5:30 PM. Although individual maps were originated from different individual participants, they cover the same anatomic area in each case. Image processing and statistical analysis were conducted with Statistical Parameter Mapping version 8 (2008, Welcome Department of Cognitive Neurology, London UK).


CONCLUSIONS
The maximal BOLD signal changes were calculated for each subject in the motor area M1; Activation maps were calculated and overlaid on the anatomical images. Group analysis of the data was performed, and the average volume and the maximum intensity of BOLD signal in the activated area M1 was determined for all studied volunteers. The current study allowed measuring regional brain volumes and neural network activity before and during an extended period of fasting using BOLD-fMRI. This demonstrated and confirmed the impact of fasting on human brain structure and function. Further studies are required to elucidate mechanisms and enable direct inference of a diet-induced OS effect on the brain.
a Department of Biophysics and Clinical MRI Methods, Faculty of Medicine and Pharmacy , University of Fez , Morocco.|b Clinical Neuroscience Laboratory, Faculty of Medicine and Pharmacy , University of Fez , Morocco.|c Laboratory of Neuroendocrinology and Nutritional and Climatic Environment, Faculty of Sciences Dhar El Mahraz , University Sidi Mohammed Ben Abdellah , Fez-Atlas , Fez BP 1796 , Morocco.
Rachida
R
Belaïch
a Department of Biophysics and Clinical MRI Methods, Faculty of Medicine and Pharmacy , University of Fez , Morocco.|b Clinical Neuroscience Laboratory, Faculty of Medicine and Pharmacy , University of Fez , Morocco.
Saïd
http://orcid.org/0000-0001-8298-6464
S
Boujraf
b Clinical Neuroscience Laboratory, Faculty of Medicine and Pharmacy , University of Fez , Morocco.
Mohammed
M
Benzagmout
c Laboratory of Neuroendocrinology and Nutritional and Climatic Environment, Faculty of Sciences Dhar El Mahraz , University Sidi Mohammed Ben Abdellah , Fez-Atlas , Fez BP 1796 , Morocco.
Rabia
R
Magoul
b Clinical Neuroscience Laboratory, Faculty of Medicine and Pharmacy , University of Fez , Morocco.|d Department of Radiology and Clinical Imaging , University Hospital of Fez , Morocco.
Mustapha
M
Maaroufi
b Clinical Neuroscience Laboratory, Faculty of Medicine and Pharmacy , University of Fez , Morocco.|d Department of Radiology and Clinical Imaging , University Hospital of Fez , Morocco.
Siham
S
Tizniti
D000328:Adult; D001794:Blood Pressure; D001921:Brain / Q000000981:diagnostic imaging / Q000378:metabolism*; D004734:Energy Metabolism; D005215:Fasting / Q000009:adverse effects*; D059907:Functional Neuroimaging; D006693:Holidays; D006801:Humans; D007514:Islam; D008279:Magnetic Resonance Imaging; D008297:Male; D008959:Models, Neurological; D009018:Morocco; D009044:Motor Cortex / Q000000981:diagnostic imaging / Q000378:metabolism; D009473:Neuronal Plasticity; D009474:Neurons / Q000378:metabolism*; D018384:Oxidative Stress
BOLD-fMRI; Brain plasticity; Oxidative stress; Restricted diet