Lack of Association between IL-10 Gene Promoter Polymorphisms and Susceptible to Tuberculosis in Thai Patients.
BACKGROUND
Cytokines play a major role in defense against Mycobacterium tuberculosis infection. Polymorphisms in the genes encoding various cytokines have been associated with tuberculosis susceptibility. Polymorphisms of the regulatoy cytokine gene, the interleukin (IL)-10 is associated with the risk of tuberculosis (TB) in different populations. However IL-10 gene polymorphism and susceptibility to TB in Thai is still unknown.


OBJECTIVE
The purpose of this study was to evaluate whether the common IL-10 promoter gene polymorphisms are associated with TB in Thai population.


METHODS
Forty-eight patients with newly diagnosed pulmonary tuberculosis were studied. DNA samples were extracted from leukocytes and used to investigate -1087A/G -819C/T -252C/A (rs1800896, rs1800871, rs1800872) in IL-10 gene using restriction fragment length polymorphism (PCR-RFLP) methods.


RESULTS
In this study, the genotype and allele frequencies of IL-10-1087A/G, -819C/T -252C/A polymorphism did not significantly different between TB patients and healthy controls ((genotype: p = 0.38, p = 0.92,p = 1; allele: p = 0.57, p = 0.77, p = 0.89, respectively).


CONCLUSIONS
The lack of association between common IL-10 promoter polymorphisms and TB susceptibility in this study may provide clue for better understanding of IL-10-1087A/G -819C/T -252C/A polymorphism and TB susceptibility in Thai population, which might facilitate the rationale design of vaccines. However further studies in large scales population are required for confirmation.
Manaphol
M
Kulpraneet
Anirut
A
Limtrakul
Surangrat
S
Srisurapanon
Piyatida
P
Tangteerawatana
D000328:Adult; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000502:physiology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D013785:Thailand / Q000453:epidemiology; D014376:Tuberculosis / Q000453:epidemiology / Q000235:genetics*
C508609:IL10 protein, human; D016753:Interleukin-10