Rapid screening and quantitative determination of bioactive compounds from fruit extracts of Myristica species and their in vitro antiproliferative activity.
Efficient and sensitive LC-MS/MS methods have been developed for the rapid screening and determination of bioactive compounds in different fruit parts of four Myristica species, viz., Myristica beddomeii, Myristica fragrans, Myristica fatua and Myristica malabarica. Twenty-one compounds were identified and characterized on the basis of their accurate mass and MS/MS fragmentation pattern using HPLC-QTOF-MS/MS and NMR analysis. Quantitative determination of five major bioactive compounds was performed using multiple-reaction monitoring mode with continuous polarity switching by UHPLC-QqQLIT-MS/MS. Moreover, in vitro antiproliferative activity of these Myristica species was evaluated against five human cancer cell lines A549, DLD-1, DU145, FaDu and MCF-7 using SRB assay. Seventeen phytoconstituents were identified and reported for the first time from M. beddomeii and sixteen from M. fatua. Quantification result showed highest total content of five major bioactive compounds in mace of M. fragrans. Evaluation of in vitro antiproliferative activity revealed potent activity in all investigated species except M. fragrans.
Sophisticated Analytical Instrument Facility, CSIR-Central Drug Research Institute, Lucknow 226031, Uttar Pradesh, India; Academy of Scientific and Innovative Research (AcSIR), New Delhi 110025, India.
Renu
R
Pandey
Sophisticated Analytical Instrument Facility, CSIR-Central Drug Research Institute, Lucknow 226031, Uttar Pradesh, India; Academy of Scientific and Innovative Research (AcSIR), New Delhi 110025, India.
Rohit
R
Mahar
Biochemistry Division, CSIR-Central Drug Research Institute, Lucknow 226031, Uttar Pradesh, India.
Mohammad
M
Hasanain
Sophisticated Analytical Instrument Facility, CSIR-Central Drug Research Institute, Lucknow 226031, Uttar Pradesh, India; Academy of Scientific and Innovative Research (AcSIR), New Delhi 110025, India.
Sanjeev K
SK
Shukla
Biochemistry Division, CSIR-Central Drug Research Institute, Lucknow 226031, Uttar Pradesh, India.
Jayanta
J
Sarkar
Phytochemistry Division, Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Palode, Thiruvananthapuram, Kerala 695562, India.
K B
KB
Rameshkumar
Sophisticated Analytical Instrument Facility, CSIR-Central Drug Research Institute, Lucknow 226031, Uttar Pradesh, India; Academy of Scientific and Innovative Research (AcSIR), New Delhi 110025, India. Electronic address: brijesh_kumar@cdri.res.in.
Brijesh
B
Kumar
D000072283:A549 Cells; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D005638:Fruit / Q000737:chemistry*; D006801:Humans; D061986:MCF-7 Cells; D026323:Myristica / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry
D010936:Plant Extracts
In vitro antiproliferative activity; LC–MS/MS; Myristica species; NMR