Infectious Bronchitis Coronavirus Limits Interferon Production by Inducing a Host Shutoff That Requires Accessory Protein 5b.
During infection of their host cells, viruses often inhibit the production of host proteins, a process that is referred to as host shutoff. By doing this, viruses limit the production of antiviral proteins and increase production capacity for viral proteins. Coronaviruses from the genera Alphacoronavirus and Betacoronavirus, such as severe acute respiratory syndrome coronavirus (SARS-CoV), establish host shutoff via their nonstructural protein 1 (nsp1). The Gammacoronavirus and Deltacoronavirus genomes, however, do not encode nsp1, and it has been suggested that these viruses do not induce host shutoff. Here, we show that the Gammacoronavirus infectious bronchitis virus (IBV) does induce host shutoff, and we find that its accessory protein 5b is indispensable for this function. Importantly, we found that 5b-null viruses, unlike wild-type viruses, induce production of high concentrations of type I interferon protein in vitro, indicating that host shutoff by IBV plays an important role in antagonizing the host's innate immune response. Altogether, we demonstrate that 5b is a functional equivalent of nsp1, thereby answering the longstanding question of whether lack of nsp1 in gammacoronaviruses is compensated for by another viral protein. As such, our study is a significant step forward in the understanding of coronavirus biology and closes a gap in the understanding of some IBV virulence strategies.



Many viruses inhibit protein synthesis by their host cell to enhance virus replication and to antagonize antiviral defense mechanisms. This process is referred to as host shutoff. We studied gene expression and protein synthesis in chicken cells infected with the important poultry pathogen infectious bronchitis virus (IBV). We show that IBV inhibits synthesis of host proteins, including that of type I interferon, a key component of the antiviral response. The IBV-induced host shutoff, however, does not require degradation of host RNA. Furthermore, we demonstrate that accessory protein 5b of IBV plays a crucial role in the onset of host shutoff. Our findings suggest that inhibition of host protein synthesis is a common feature of coronaviruses and primarily serves to inhibit the antiviral response of the host.
Cell Biology and Immunology Group, Wageningen Institute of Animal Sciences, Wageningen University, Wageningen, The Netherlands.|MSD Animal Health, Bioprocess Technology and Support, Boxmeer, The Netherlands.
Joeri
J
Kint
Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
Martijn A
MA
Langereis
Avian Viral Diseases, The Pirbright Institute, Compton Laboratory, Pirbright, United Kingdom.
Helena J
HJ
Maier
Avian Viral Diseases, The Pirbright Institute, Compton Laboratory, Pirbright, United Kingdom.
Paul
P
Britton
Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
Frank J
FJ
van Kuppeveld
MSD Animal Health, Bioprocess Technology and Support, Boxmeer, The Netherlands.
Joseph
J
Koumans
Cell Biology and Immunology Group, Wageningen Institute of Animal Sciences, Wageningen University, Wageningen, The Netherlands.
Geert F
GF
Wiegertjes
Cell Biology and Immunology Group, Wageningen Institute of Animal Sciences, Wageningen University, Wageningen, The Netherlands maria.forlenza@wur.nl.
Maria
http://orcid.org/0000-0001-9026-7320
M
Forlenza
D000818:Animals; D002478:Cells, Cultured; D002645:Chickens; D055786:Gene Knockout Techniques; D054884:Host-Pathogen Interactions; D057131:Immune Evasion; D001351:Infectious bronchitis virus / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity*; D007370:Interferon Type I / Q000037:antagonists & inhibitors*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D007370:Interferon Type I; D014764:Viral Proteins