PCE dechlorination by non-Dehalococcoides in a microbial electrochemical system.
The bioremediation of tetrachloroethene (perchloroethene; PCE) contaminated sites generally requires a supply of some fermentable organic substrates as an electron donor. On the other hand, organic substrates can induce the massive growth of microorganisms around the injection wells, which can foul the contaminated subsurface environment. In this study, PCE dechlorination to ethene was performed in a microbial electrochemical system (MES) using the electrode (a cathode polarized at -500 mV vs. standard hydrogen electrode) as the electron donor. Denaturing gel gradient electrophoresis and pyrosequencing revealed a variety of non-Dehalococcoides bacteria dominant in MES, such as Acinetobacter sp. (25.7 % for AS1 in suspension of M3), Rhodopseudomonas sp. (10.5 % for AE1 and 10.1 % for AE2 in anodic biofilm of M3), Pseudomonas aeruginosa (22.4 % for BS1 in suspension of M4), and Enterobacter sp. (21.7 % for BE1 in anodic biofilm of M4) which are capable of electron transfer, hydrogen production and dechlorination. The Dehalococcoides group, however, was not detected in this system. Therefore, these results suggest that a range of bacterial species outside the Dehalococcoides can play an important role in the microbial electrochemical dechlorination process, which may lead to innovative bioremediation technology.
Department of Civil and Environmental Engineering, Pusan National University, Busan, 46241, Korea.
Jaecheul
J
Yu
Department of Civil and Environmental Engineering, Pusan National University, Busan, 46241, Korea.
Younghyun
Y
Park
Department of Civil and Environmental Engineering, Pusan National University, Busan, 46241, Korea.
Van Khanh
VK
Nguyen
Department of Civil and Environmental Engineering, Pusan National University, Busan, 46241, Korea. leeth55@pusan.ac.kr.
Taeho
T
Lee
D001419:Bacteria / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D041862:Chloroflexi / Q000302:isolation & purification; D055664:Electrochemical Techniques; D004579:Electron Transport; D004785:Environmental Pollutants / Q000737:chemistry / Q000378:metabolism*; D005030:Ethylenes / Q000378:metabolism; D013750:Tetrachloroethylene / Q000737:chemistry / Q000378:metabolism*
D004785:Environmental Pollutants; D005030:Ethylenes; C036216:ethylene; D013750:Tetrachloroethylene
Biocathode; Microbial community; Microbial electrochemical dechlorination; PCE; Pyrosequencing