Assessment of oxidative stress after out-of-hospital cardiac arrest.
BACKGROUND
Pathophysiology of cardiac arrest corresponds to a whole body ischemia-reperfusion. This phenomenon is usually associated with an oxidative stress in various settings, but few data are available on cardiac arrest in human. The aim of the present study was to evaluate different oxidative stress markers in out-of-hospital cardiac arrest (OHCA) patients treated with therapeutic hypothermia.


METHODS
We conducted a prospective study assessing oxidative stress markers (thiobarbituric acid reactive species, carbonyls, thiols, glutathione, and glutathione peroxidase) in OHCA patients treated with therapeutic hypothermia. Measurements were performed during the 4 days after admission and compared between good and poor outcome patients according to Cerebral Performance Category.


RESULTS
Thirty-four patients were included, 10 good and 24 poor outcomes at 6 months. Thiobarbituric acid reactive species were higher in the poor outcome group on admission and when therapeutic hypothermia was reached. The other markers were not different between groups. No markers seemed modified by the use of therapeutic hypothermia in each group.


CONCLUSIONS
After OHCA, good outcome patients exhibit lower oxidative stress markers than poor outcome patients. Thiobarbituric acid reactive species appears to be an early prognostic parameter. Oxidative stress markers seem not mitigated by therapeutic hypothermia.
Intensive Care Unit, Pasteur Hospital, Nice University Hospital, Nice, France. Electronic address: orban.jc@chu-nice.fr.
Jean-Christophe
JC
Orban
Pathology and Biology Institute, Grenoble University Hospital, Grenoble, France.
Catherine
C
Garrel
Intensive Care Unit, Pasteur Hospital, Nice University Hospital, Nice, France.
Didier
D
Déroche
Intensive Care Unit, Pasteur Hospital, Nice University Hospital, Nice, France.
Florian
F
Cattet
Biochemistry and Hormonology Laboratory, Pasteur Hospital, Nice University Hospital, Nice, France.
Patricia
P
Ferrari
Department of Medical Information, Cimiez Hospital, Nice University Hospital, France.
Frédéric
F
Berthier
Intensive Care Unit, Pasteur Hospital, Nice University Hospital, Nice, France.
Carole
C
Ichai
D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood*; D016887:Cardiopulmonary Resuscitation / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D058687:Out-of-Hospital Cardiac Arrest / Q000097:blood* / Q000628:therapy; D018384:Oxidative Stress; D011379:Prognosis; D011446:Prospective Studies; D055815:Young Adult
D015415:Biomarkers