Paracoccusacridae sp. nov., isolated from the insect Acrida cinerea living in deserted cropland.
A Gram-stain-negative, aerobic, rod-shaped, non-motile bacterial strain, designated SCU-M53T, was isolated from the insect Acrida cinerea. Phylogenetic analysis on the basis of 16S rRNA gene sequences showed that strain SCU-M53T belonged to the genus Paracoccus, having Paracoccus chinensisNBRC 104937T (97.04 % 16S rRNA gene sequence similarity) and Paracoccus niistensis KCTC 22789T (96.70 %) as the most closely related phylogenetic neighbours. Growth occurred at 10-40 °C (optimum 25-30 °C), pH 5.0-10.0 (optimum pH 6.5-7.5) and with 0-4 % (w/v) NaCl (optimum 0-1 % NaCl). The fatty acids of strain SCU-M53T were C18 : 1ω7c, C18 : 0, C16 : 0, C14 : 0 3-OH, C14 : 0 and C10 : 0 3-OH. The polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, an unknown aminolipid, two unknown phospholipids and two unknown lipids. The isoprenoid quinone was ubiquinone-10. The DNA G+C content was 60.6 mol%. Strain SCU-M53T exhibited 36.5 and 28.6 % DNA-DNA relatedness to P. chinensis NBRC 104937T and P. niistensis KCTC 22789T, respectively. According to these results, strain SCU-M53T represents a novel species of the genus Paracoccus, for which the name Paracoccus acridae sp. nov. is proposed. The type strain is SCU-M53T (=KCTC 42932T=CGMCC 1.15419T).
Key Laboratory of Leather Chemistry and Engineering (Sichuan University), Ministry of Education and College of Light Industry, Textile & Food Engineering, Sichuan University, Chengdu 610065, PR China.
Shuai
S
Zhang
Key Laboratory of Leather Chemistry and Engineering (Sichuan University), Ministry of Education and College of Light Industry, Textile & Food Engineering, Sichuan University, Chengdu 610065, PR China.
Longzhan
L
Gan
Key Laboratory of Leather Chemistry and Engineering (Sichuan University), Ministry of Education and College of Light Industry, Textile & Food Engineering, Sichuan University, Chengdu 610065, PR China.
Qiumian
Q
Qin
Key Laboratory of Leather Chemistry and Engineering (Sichuan University), Ministry of Education and College of Light Industry, Textile & Food Engineering, Sichuan University, Chengdu 610065, PR China.
Xiufeng
X
Long
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & PeKing Union Medical College, Beijing 100050, PR China.
Yuqin
Y
Zhang
Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu 610052, PR China.
Yiwen
Y
Chu
Key Laboratory of Leather Chemistry and Engineering (Sichuan University), Ministry of Education and College of Light Industry, Textile & Food Engineering, Sichuan University, Chengdu 610065, PR China.
Yongqiang
Y
Tian
D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D018556:Crops, Agricultural; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D009987:Orthoptera / Q000382:microbiology*; D010230:Paracoccus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2