Regeneration of rat corpora cavernosa tissue by transplantation of CD133+cells derived from human bone marrow and placement of biodegradable gel sponge sheet.
The objective is to develop an easier technique for regenerating corpora cavernosa tissue through transplantation of human bone marrow-derived CD133 + cells into a rat corpora cavernosa defect model. We excised 2 mm × 2 mm squares of the right corpora cavernosa of twenty-three 8-week-old male nude rats. Alginate gel sponge sheets supplemented with 1 × 10 4 CD133 + cells were then placed over the excised area of nine rats. Functional and histological evaluations were carried out 8 weeks later. The mean intracavernous pressure/mean arterial pressure ratio for the nine rats (0.34258 ± 0.0831) was significantly higher than that for eight rats with only the excision (0.0580 ± 0.0831, P = 0.0238) and similar to that for five rats for which the penis was exposed, and there was no excision (0.37228 ± 0.1051, P = 0.8266). Immunohistochemical analysis revealed that the nine fully treated rats had venous sinus-like structures and quantitative reverse transcription polymerase chain reaction analysis of extracts from their alginate gel sponge sheets revealed that the amounts of mRNA encoding the nerve growth factor (NGF), and vascular endothelial growth factor (VEGF) were significantly higher than those for rats treated with alginate gel sheets without cell supplementation (NGF: P = 0.0309; VEGF: P < 0.0001). These findings show that transplantation of CD133 + cells accelerates functional and histological recovery in the corpora cavernosa defect model.
Department of Urology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima, Japan.
Shogo
S
Inoue
Department of Urology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima, Japan.
Katsutoshi
K
Miyamoto
Department of Urology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima, Japan.|Department of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima, Japan.
Shunsuke
S
Shinmei
Department of Urology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima, Japan.
Koichi
K
Shoji
Department of Urology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima, Japan.
Jun
J
Teishima
Department of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima, Japan.
Kazuhiro
K
Sentani
Department of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima, Japan.
Wataru
W
Yasui
Department of Urology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima, Japan.
Akio
A
Matsubara
D000071916:AC133 Antigen / Q000378:metabolism; D020341:Absorbable Implants; D000199:Actins / Q000378:metabolism; D000464:Alginates / Q000627:therapeutic use*; D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism; D016026:Bone Marrow Transplantation / Q000379:methods*; D005782:Gels / Q000627:therapeutic use*; D020723:Glucuronic Acid / Q000627:therapeutic use; D006603:Hexuronic Acids / Q000627:therapeutic use; D006801:Humans; D008297:Male; D020932:Nerve Growth Factor / Q000235:genetics; D010410:Penile Erection; D010413:Penis / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D011923:Rats, Nude; D012038:Regeneration; D020133:Reverse Transcriptase Polymerase Chain Reaction; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics
D000071916:AC133 Antigen; D000199:Actins; D000464:Alginates; D005782:Gels; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D020932:Nerve Growth Factor; D042461:Vascular Endothelial Growth Factor A; C000606023:PROM1 protein, human; C498862:Acta2 protein, rat; C467498:vascular endothelial growth factor A, rat