Live cell imaging to understand monocyte, macrophage, and dendritic cell function in atherosclerosis.
Intravital imaging is an invaluable tool for understanding the function of cells in healthy and diseased tissues. It provides a window into dynamic processes that cannot be studied by other techniques. This review will cover the benefits and limitations of various techniques for labeling and imaging myeloid cells, with a special focus on imaging cells in atherosclerotic arteries. Although intravital imaging is a powerful tool for understanding cell function, it alone does not provide a complete picture of the cell. Other techniques, such as flow cytometry and transcriptomics, must be combined with intravital imaging to fully understand a cell's phenotype, lineage, and function.
Division of Inflammation Biology and Microscopy Core, La Jolla Institute of Allergy and Immunology, La Jolla, CA 92037.
Sara
S
McArdle
Division of Inflammation Biology and Microscopy Core, La Jolla Institute of Allergy and Immunology, La Jolla, CA 92037.
Zbigniew
Z
Mikulski
Division of Inflammation Biology and Microscopy Core, La Jolla Institute of Allergy and Immunology, La Jolla, CA 92037 klaus@lji.org.
Klaus
K
Ley
D000818:Animals; D050197:Atherosclerosis / Q000000981:diagnostic imaging*; D003713:Dendritic Cells / Q000473:pathology*; D006801:Humans; D000069416:Intravital Microscopy / Q000379:methods*; D008264:Macrophages / Q000473:pathology*; D009000:Monocytes / Q000473:pathology*