The comparative effects of aminoglycoside antibiotics and muscle relaxants on electrical field stimulation response in rat bladder smooth muscle.
It has been reported that several aminoglycoside antibiotics have a potential of prolonging the action of non-depolarizing muscle relaxants by drug interactions acting pre-synaptically to inhibit acetylcholine release, but antibiotics itself also have a strong effect on relaxing the smooth muscle. In this study, four antibiotics of aminoglycosides such as gentamicin, streptomycin, kanamycin and neomycin were compared with skeletal muscle relaxants baclofen, tubocurarine, pancuronium and succinylcholine, and a smooth muscle relaxant, papaverine. The muscle strips isolated from the rat bladder were stimulated with pulse trains of 40 V in amplitude and 10 s in duration, with pulse duration of 1 ms at the frequency of 1-8 Hz, at 1, 2, 4, 6, 8 Hz respectively. To test the effect of four antibiotics on bladder smooth muscle relaxation, each of them was treated cumulatively from 1 μM to 0.1 mM with an interval of 5 min. Among the four antibiotics, gentamicin and neomycin inhibited the EFS response. The skeletal muscle relaxants (baclofen, tubocurarine, pancuronium and succinylcholine) and inhibitory neurotransmitters (GABA and glycine) did not show any significant effect. However, papaverine, had a significant effect in the relaxation of the smooth muscle. It was suggested that the aminoglycoside antibiotics have inhibitory effect on the bladder smooth muscle.
College of Pharmacy, Chung-Ang University, Seoul, 156-756, Republic of Korea.
Chang Ho
CH
Min
Department of Medical Plant Science, Jung Won University, Chungbuk, Republic of Korea.
Young Sil
YS
Min
College of Pharmacy, Chung-Ang University, Seoul, 156-756, Republic of Korea. sjlee5029@cau.ac.kr.
Sang Joon
SJ
Lee
College of Pharmacy, Chung-Ang University, Seoul, 156-756, Republic of Korea. udsohn@cau.ac.kr.
Uy Dong
UD
Sohn
D000617:Aminoglycosides / Q000494:pharmacology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D066298:In Vitro Techniques; D008297:Male; D009125:Muscle Relaxants, Central / Q000494:pharmacology*; D009126:Muscle Relaxation / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects*; D017207:Rats, Sprague-Dawley; D001743:Urinary Bladder / Q000187:drug effects*
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D009125:Muscle Relaxants, Central
GABA; NANC; Non-adrenergic; Non-cholinergic transmitter; γ-Aminobutyric acid