Antibody to fibroblast growth factor 23-peptide reduces excreta phosphorus of laying hens.
Novel strategies to minimize the excretion of phosphorus in swine and poultry are critical in minimizing environmental degradation. We have developed a synthetic peptide vaccine to produce autoantibodies to fibroblast growth factor 23 (FGF-23), a bone-derived hormone that blocks kidney phosphate resorption and indirectly reduces intestinal phosphate absorption. Single Comb White Leghorn laying hens, fed a standard diet (inorganic phosphorus, Pi = 0.4%), were immunized over the course of 4 weeks with either a FGF-23 peptide vaccine or adjuvant control (without FGF-23 peptide). At peak antibody titer to the peptide (week 5), 24-h excreta were collected and hens were blood sampled (represents 0.4% Pi treatment). Hens were then fed a 0.8% Pi diet and blood was sampled at 24 and 72 h and 24-h excreta were collected at 12 to 36 and 60 to 84 h (represents 0.8% Pi treatment). Increasing Pi from 0.4 to 0.8% increased (P < 0.05) percent excreta phosphorus, total 24-h phosphorus excretion, and plasma levels of FGF-23 and phosphate in either control or FGF-23 peptide vaccinated hens as early as the first sampling period. FGF-23 peptide vaccinated hens fed 0.4% Pi had reduced (P < 0.05) percent excreta phosphorus, total 24 h phosphorus excretion, and plasma levels of FGF-23 and iPTH, and increased (P < 0.05) plasma levels of phosphate and 1,25(OH)2D3when compared to control vaccinated hens fed 0.4% Pi. In the first collection period post 0.8% Pi feeding, FGF-23 peptide vaccinated hens had reduced (P < 0.05) plasma levels of FGF-23 and iPTH, and increased (P < 0.05) plasma levels of phosphate and 1,25(OH)2D3, and tended to have reduced percent excreta phosphorus (P = 0.085) and total 24 h phosphorus excretion (P = 0.078) when compared to control vaccinated hens. Results during the second collection period post 0.8% Pi feeding were similar to that at the first collection period. These results are the first to show that the inhibition of FGF-23 action by a peptide vaccine (via neutralizing antibody) reduced phosphorus excretion. The approach presented provides new information on phosphorus metabolism in the laying hen.
Department of Animal Sciences, University of Wisconsin-Madison, Madison, WI 53706, USA.|Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Zhouzheng
Z
Ren
Department of Animal Science, University of Tabriz, Tabriz 5166616471, Iran.
Marziyeh
M
Ebrahimi
Department of Animal Sciences, University of Wisconsin-Madison, Madison, WI 53706, USA.
Daniel E
DE
Bütz
Department of Animal Sciences, University of Wisconsin-Madison, Madison, WI 53706, USA.
Jordan M
JM
Sand
Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Keying
K
Zhang
Department of Animal Sciences, University of Wisconsin-Madison, Madison, WI 53706, USA mcook@wisc.edu.
Mark E
ME
Cook
D000818:Animals; D001323:Autoantibodies / Q000378:metabolism*; D030161:Avian Proteins / Q000378:metabolism*; D002645:Chickens / Q000502:physiology*; D005260:Female; D000089703:Fibroblast Growth Factor-23; D005346:Fibroblast Growth Factors / Q000378:metabolism*; D006706:Homeostasis; D006728:Hormones / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism*; D014612:Vaccines / Q000008:administration & dosage
D001323:Autoantibodies; D030161:Avian Proteins; D006728:Hormones; D014612:Vaccines; D010758:Phosphorus; D005346:Fibroblast Growth Factors; D000089703:Fibroblast Growth Factor-23
FGF-23; antibody; excreta phosphorus; laying hen; peptide vaccine