Combination of Xylanase and Debranching Enzymes Specific to Wheat Arabinoxylan Improve the Growth Performance and Gut Health of Broilers.
Arabinoxylan (AX) is the major antinutritional factor of wheat. This study evaluated the synergistic effects of xylanase and debranching enzymes (arabinofuranosidase [ABF] and feruloyl esterase [FAE]) on AX. During in vitro tests, the addition of ABF or FAE accelerated the hydrolysis of water-soluble AX (WE-AX) and water-insoluble AX (WU-AX) and produced more xylan oligosaccharides (XOS) than xylanase alone. XOS obtained from WE-AX stimulated greater proliferation of Lactobacillus brevis and Bacillus subtilis than did fructo-oligosaccharides (FOS) and glucose. During in vivo trials, xylanase increased the average daily growth (ADG), decreased the feed-conversion ratio (FCR), and reduced the digesta viscosity of jejunum and intestinal lesions of broilers fed a wheat-based diet on day 36. ABF or FAE additions further improved these effects. Broilers fed a combination of xylanase, ABF, and FAE exhibited the best growth. In conclusion, the synergistic effects among xylanase, ABF, and FAE increased AX degradation, which improve the growth performance and gut health of broilers.
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University , 2 Yuanmingyuan West Road, Beijing, 100193, PR China.
Zhao
Z
Lei
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University , 2 Yuanmingyuan West Road, Beijing, 100193, PR China.
Yuxin
Y
Shao
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University , 2 Yuanmingyuan West Road, Beijing, 100193, PR China.
Xiaonan
X
Yin
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University , 2 Yuanmingyuan West Road, Beijing, 100193, PR China.
Dafei
D
Yin
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University , 2 Yuanmingyuan West Road, Beijing, 100193, PR China.
Yuming
Y
Guo
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University , 2 Yuanmingyuan West Road, Beijing, 100193, PR China.
Jianmin
J
Yuan
D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D001412:Bacillus subtilis / Q000254:growth & development / Q000378:metabolism; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D002645:Chickens / Q000254:growth & development / Q000378:metabolism*; D004063:Digestion; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism*; D005503:Food Additives / Q000378:metabolism; D000069196:Gastrointestinal Microbiome; D006026:Glycoside Hydrolases / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000201:enzymology / Q000382:microbiology; D052196:Levilactobacillus brevis / Q000254:growth & development / Q000378:metabolism; D014908:Triticum / Q000378:metabolism*; D014990:Xylans / Q000378:metabolism*
D005503:Food Additives; D014990:Xylans; C085118:arabinoxylan; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase; D006026:Glycoside Hydrolases; D043364:Endo-1,4-beta Xylanases
broilers; debranching enzymes; growth performance; gut health; synergistic effect; xylan oligosaccharides; xylanase