Structure and function of the Mind bomb E3 ligase in the context of Notch signal transduction.
The Notch signaling pathway has a critical role in cell fate determination and tissue homeostasis in a variety of different lineages. In the context of normal Notch signaling, the Notch receptor of the 'signal-receiving' cell is activated in trans by a Notch ligand from a neighboring 'signal-sending' cell. Genetic studies in several model organisms have established that ubiquitination of the Notch ligand, and its regulated endocytosis, is essential for transmission of this activation signal. In mammals, this ubiquitination step is dependent on the protein Mind bomb 1 (Mib1), a large multi-domain RING-type E3 ligase, and its direct interaction with the intracellular tails of Notch ligand molecules. Here, we discuss our current understanding of Mind bomb structure and mechanism in the context of Notch signaling and beyond.
Harvard Medical School, Department of Biological Chemistry and Molecular Pharmacology, Boston, MA 02115, United States.
Bingqian
B
Guo
Harvard Medical School, Department of Biological Chemistry and Molecular Pharmacology, Boston, MA 02115, United States; Broad Institute, Center for Development of Therapeutics, Cambridge, MA 02142, United States.
Brian J
BJ
McMillan
Harvard Medical School, Department of Biological Chemistry and Molecular Pharmacology, Boston, MA 02115, United States; Dana Farber Cancer Institute, Department of Cancer Biology, Boston, MA 02115, United States. Electronic address: stephen_blacklow@hms.harvard.edu.
Stephen C
SC
Blacklow
D000818:Animals; D006801:Humans; D000072417:Protein Domains; D051880:Receptors, Notch / Q000378:metabolism*; D015398:Signal Transduction; D025801:Ubiquitin / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000737:chemistry* / Q000378:metabolism*
D051880:Receptors, Notch; D025801:Ubiquitin; D044767:Ubiquitin-Protein Ligases