Cholinesterases as markers of the inflammatory process associated oxidative stress in cattle infected by Babesia bigemina.
The objective of this study was to assess the influence of an asymptomatic experimental infection by Babesia bigemina on cholinesterase's as markers of the inflammatory process and biomarkers of oxidative imbalance. For this purpose, eight naive animals were used, as follows: four as controls or uninfected; and four infected with an attenuated strain of B. bigemina. Blood samples were collected on days 0, 7 and 11 post-inoculation (PI). Parasitemia was determined by blood smear evaluation, showing that the infection by B. bigemina resulted in mean 0.725 and 0.025% on day 7 and 11 PI, respectively, as well as mild anemia. The activities of acetylcholinesterase, butyrylcholinesterase and catalase were lower, while levels of thiobarbituric acid reactive substances and superoxide dismutase activity were higher in infected animals, when compared with the control group. This attenuated strain of B. bigemina induced an oxidative stress condition, as well as it reduces the cholinesterasés activity in infected and asymptomatic cattle. Therefore, this decrease of cholinesterase in infection by B. bigemina purpose is to inhibit inflammation, for thereby increasing acetylcholine levels, potent anti-inflammatory molecules.
Graduate Program in Veterinary Medicine, Department of Small Animal, Veterinary Hospital, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil; Graduate Program in Biological Sciences: Toxicological Biochemistry, Laboratory of Toxicology Enzymology, Department of Chemistry, Centro de Ciências Naturais e Exatas; Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Rovaina L
RL
Doyle
Department of Animal Science, Universidade do Estado de Santa Catarina, Chapecó, SC, Brazil.
Aleksandro S
AS
Da Silva
Graduate Program in Veterinary Medicine, Department of Microbiology and Parasitology, Prédio 20, Universidade Federal de Santa Maria, Santa Maria , RS, Brazil.
Camila B
CB
Oliveira
Graduate Program in Veterinary Medicine, Department of Small Animal, Veterinary Hospital, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Raqueli T
RT
França
Graduate Program in Biological Sciences: Toxicological Biochemistry, Division of Biochemistry and Oxidative Stress, Laboratory of Cell Therapy, Centro de Ciências Rurais, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Fabiano B
FB
Carvalho
Graduate Program in Biological Sciences: Toxicological Biochemistry, Division of Biochemistry and Oxidative Stress, Laboratory of Cell Therapy, Centro de Ciências Rurais, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Fátima H
FH
Abdalla
Graduate Program in Biological Sciences: Toxicological Biochemistry, Laboratory of Toxicology Enzymology, Department of Chemistry, Centro de Ciências Naturais e Exatas; Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Pauline
P
Costa
Instituto de Pesquisas Veterinárias Desidério Finamor, Fundação Estadual de Pesquisa Agropecuária (FEPAGRO), Eldorado do Sul, RS , Brazil.
Guilherme M
GM
Klafke
Instituto de Pesquisas Veterinárias Desidério Finamor, Fundação Estadual de Pesquisa Agropecuária (FEPAGRO), Eldorado do Sul, RS , Brazil.
João R
JR
Martins
Graduate Program in Veterinary Medicine, Department of Microbiology and Parasitology, Prédio 20, Universidade Federal de Santa Maria, Santa Maria , RS, Brazil.
Alexandre A
AA
Tonin
Graduate Program in Veterinary Medicine, Department of Small Animal, Veterinary Hospital, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Verônica S P
VS
Castro
Centre for Biological Sciences, Department of Epidemiology and Microbiology, Health Sciences, Universidade Federal do Piauí, Campus Senador Helvídio Nunes de Barros,Picos, PI , 64600-000, Brazil.
Franklin G B
FG
Santos
Graduate Program in Veterinary Medicine, Department of Small Animal, Veterinary Hospital, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Sonia T A
ST
Lopes
Graduate Program in Veterinary Medicine, Department of Small Animal, Veterinary Hospital, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil; Graduate Program in Biological Sciences: Toxicological Biochemistry, Division of Biochemistry and Oxidative Stress, Laboratory of Cell Therapy, Centro de Ciências Rurais, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil. Electronic address: cmelazzo@yahoo.com.br.
Cinthia M
CM
Andrade
D000818:Animals; D001403:Babesia / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D001404:Babesiosis / Q000378:metabolism* / Q000469:parasitology; D015415:Biomarkers / Q000097:blood; D002374:Catalase / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000378:metabolism* / Q000469:parasitology; D002802:Cholinesterases / Q000097:blood*; D016054:DNA, Protozoan / Q000097:blood; D007249:Inflammation; D015227:Lipid Peroxidation; D018384:Oxidative Stress; D018512:Parasitemia; D016133:Polymerase Chain Reaction; D013482:Superoxide Dismutase / Q000097:blood; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D015415:Biomarkers; D016054:DNA, Protozoan; D017392:Thiobarbituric Acid Reactive Substances; D002374:Catalase; D013482:Superoxide Dismutase; D002802:Cholinesterases
Acetylcholinesterase; Babesiosis; Catalase; Lipid peroxidation; Superoxide dismutase