High density lipoprotein subfractions and paraoxonase 1 in children.
The Lipoprint system (Quantimetrix Corp., CA, USA), enables the determination of 10 high density lipoprotein (HDL) subfractions in contrast to the 5 HDL subfractions that can be determined by ultracentrifuge analysis. HDL subfractions, and their relationships to the arylesterase (PON1-A) and lactonase (PON1-L) activities of paraoxonase 1 (PON1), together with total-, very low density lipoprotein- and low density lipoprotein (LDL)-cholesterol and LDL subfractions were investigated in the serum of 27 mildly hypercholesterolemic children and 21 healthy controls. Our results suggest the antiatherogenity of large HDL (L-HDL) subfractions and the atherogenity of small HDL (S-HDL) subfractions in the study groups. However, the relationship between the intermediate HDL (I-HDL) subfractions with the LDL subfractions and other lipoproteins did not suggest that I-HDL subfractions are antiatherogenic. No significant association between PON1-A and the HDL subfractions was found. In contrast, PON1-L activity positively correlated with the antiatherogenic large HDL1 subfraction and negatively with intermediate HDL subfractions 4, 5 and 6. Our results contribute to the knowledge of the roles of total HDL and ten individual HDL subfractions in children and adolescents.
Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Comenius University, Bratislava, Slovakia.
Jana
J
Muchová
Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Comenius University, Bratislava, Slovakia.
Lucia
L
Andrezálová
2nd Department of Internal Medicine, Faculty of Medicine, Comenius University, Bratislava, Slovakia.
Stanislav
S
Oravec
Juvenalia Paediatric Centre, Dunajská Streda, Slovakia.
Zuzana
Z
Nagyová
Research Department, Cultech Ltd, Unit, Port Talbot SA12 7BZ, UK.
Iveta
I
Garaiova
Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Comenius University, Bratislava, Slovakia.
Zdeňka
Z
Ďuračková
D000293:Adolescent; D043303:Aryldialkylphosphatase / Q000097:blood*; D016022:Case-Control Studies; D002648:Child; D005260:Female; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood / Q000201:enzymology*; D008075:Lipoproteins, HDL / Q000097:blood*; D008297:Male; D010865:Pilot Projects
D008075:Lipoproteins, HDL; D043303:Aryldialkylphosphatase; C468406:PON1 protein, human