Simultaneous chromate and sulfate removal by Streptomyces sp. MC1. Changes in intracellular protein profile induced by Cr(VI).
The purpose of this study was to investigate the influence of increasing sulfate concentrations on chromium removal, to evaluate the effect of the presence of Cr(VI) on sulfate removal by Streptomyces sp. MC1 and to analyze the differential protein expression profile in the presence of this metal for the identification of proteins repressed or overexpressed. In the presence of Cr(VI) but in the absence of sulfate ions, bacterial growth was negligible, showing the Cr(VI) toxicity for this bacterium. However, the sulfate presence stimulated bacterium growth and Cr(VI) removal, regardless of its concentrations. Streptomyces sp. MC1 showed ability to remove chromium and sulfate simultaneously. Also, the sulfate presence favored the decrease of total chromium concentration from supernatants reaching a decrease of 50% at 48 h. In presence of chromium, seven proteins were down-expressed and showed homology to proteins involved in protein biosynthesis, energy production and free radicals detoxification while two proteins involved in oxidation-reduction processes identified as dihydrolipoamide dehydrogenase and S-adenosyl-l-methionine synthase were overexpressed.
Instituto de Quimica San Luis (INQUISAL) CONICET, San Luis, Argentina.|Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, San Luis, Argentina.
José Oscar
JO
Bonilla
Division of Basic Biomedical Sciences Sanford School of Medicine, University of South Dakota, Vermillion, SD, USA.
Eduardo Alberto
EA
Callegari
Instituto de Quimica San Luis (INQUISAL) CONICET, San Luis, Argentina.
Claudio Daniel
CD
Delfini
Planta Piloto de Procesos Industriales y Microbiológicos (PROIMI), CONICET, Tucumán, Argentina.|Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Tucumán, Argentina.
María Cristina
MC
Estevez
Instituto de Quimica San Luis (INQUISAL) CONICET, San Luis, Argentina.|Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, San Luis, Argentina.
Liliana Beatriz
LB
Villegas
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001673:Biodegradation, Environmental; D018533:Biomass; D002857:Chromium / Q000378:metabolism* / Q000633:toxicity*; D019295:Computational Biology; D003470:Culture Media / Q000737:chemistry; D008058:Dihydrolipoamide Dehydrogenase / Q000235:genetics / Q000378:metabolism; D007220:Industrial Waste; D010084:Oxidation-Reduction; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000737:chemistry; D013302:Streptomyces / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D013431:Sulfates / Q000378:metabolism* / Q000494:pharmacology
D001426:Bacterial Proteins; D003470:Culture Media; D007220:Industrial Waste; D012989:Soil Pollutants; D013431:Sulfates; D002857:Chromium; C074702:chromium hexavalent ion; D008058:Dihydrolipoamide Dehydrogenase
Actinobacteria; Chromium removal; Sulfate removal