Effect of biomimetic shear stress on intracellular uptake and cell-killing efficiency of doxorubicin in a free and liposomal formulation.
Shear stress could be considered in the context of cellular uptake and cell-killing efficiency. Thus, mimicking the dynamic characteristics of in vivo environment is important in targeted drug delivery. We investigated the intracellular uptake and cell-killing efficiency of doxorubicin (DOX) in a free and liposomal form (Doxil(®)) under biomimetic shear stress to mimic in vivo environment. In this dynamic environment, cells demonstrated significantly higher fluorescence intensity than that of the static environment, and fluorescence microscopy images indicated increased intracellular uptake of DOX in the presence of fluidic shear stress. In cells treated with free DOX and liposomal Doxil(®), cell-killing efficiency was affected by shear stress. Taken together, shear stress, affecting drug uptake and cell-killing efficiency, is important in intracellular drug targeting.
College of Pharmacy, Ajou University, Suwon 443-749, Republic of Korea.
Taehee
T
Kang
College of Pharmacy, Ajou University, Suwon 443-749, Republic of Korea.
Younhee
Y
Cho
College of Pharmacy, Ajou University, Suwon 443-749, Republic of Korea.
Chulhun
C
Park
College of Pharmacy, Ajou University, Suwon 443-749, Republic of Korea.
Soo-Dong
SD
Kim
College of Pharmacy, The Catholic University, Bucheon 420-743, Republic of Korea.
Euichaul
E
Oh
College of Pharmaceutical Science, Soochow University, Suzhou, 215123, China.
Jing-Hao
JH
Cui
College of Pharmaceutical Science, Soochow University, Suzhou, 215123, China.
Qing-Ri
QR
Cao
College of Pharmacy, Ajou University, Suwon 443-749, Republic of Korea; Institute of Pharmaceutical Science and Technology, Ajou University, Suwon 16499, Republic of Korea. Electronic address: bjl@ajou.ac.kr.
Beom-Jin
BJ
Lee
D000072283:A549 Cells; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D040761:Biomimetic Materials / Q000737:chemistry / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002626:Chemistry, Pharmaceutical; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D057809:HEK293 Cells; D019073:HT29 Cells; D006801:Humans; D007424:Intracellular Fluid / Q000187:drug effects* / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology; D033081:Shear Strength; D013314:Stress, Mechanical
C506643:liposomal doxorubicin; D011092:Polyethylene Glycols; D004317:Doxorubicin
Biomimetic dynamic conditions; Cell microenvironment; Cell-killing efficiency; Cellular drug delivery; Doxorubicin; Liposomal formulation; Shear stress