Inhibition of pentraxin 3 in glioma cells impairs proliferation and invasion in vitro and in vivo.
Pentraxin 3 (PTX3) is an inflammatory molecule that is involved in immune responses, inflammation, and cancer. Recent evidence suggests that PTX3 plays a critical role in tumor progression; however, its impact on the biological function of gliomas remains unknown. In the present study, immunohistochemical staining showed that patients with high-grade gliomas exhibited increased expression levels of PTX3 compared to those with low-grade gliomas (P < 0.001). Furthermore, knockdown of PTX3 in GBM8401 cells inhibits proliferation, increases p21 protein levels, and decreases cyclin D1 protein levels, resulting in cell cycle arrest at the G0/G1 phase. In addition, knockdown of PTX3 significantly decreases GBM8401 cell migration and invasion through the downregulation of matrix metalloproteinase-1 and -2 (MMP-1 and MMP-2) expression. In a GBM8401 xenograft animal model, PTX3 knockdown decreases tumor growth in vivo. In conclusion, PTX3 plays an important role in glioma cell proliferation and invasion, and may thus serve as a novel potential therapeutic target in the treatment of gliomas.
Department of Neurosurgery, Tungs' Taichung MetroHarbor Hospital, Taichung, Taiwan.
Jai-Nien
JN
Tung
Department of Neurosurgery, Tungs' Taichung MetroHarbor Hospital, Taichung, Taiwan.|Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.
Chung-Po
CP
Ko
Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.
Shun-Fa
SF
Yang
Institute of Biochemistry, Microbiology and Immunology, Chung Shan Medical University, Taichung, Taiwan.
Chun-Wen
CW
Cheng
Institute of Biochemistry, Microbiology and Immunology, Chung Shan Medical University, Taichung, Taiwan.
Pei-Ni
PN
Chen
Institute of Biochemistry, Microbiology and Immunology, Chung Shan Medical University, Taichung, Taiwan.
Chia-Yu
CY
Chang
Institute of Biochemistry, Microbiology and Immunology, Chung Shan Medical University, Taichung, Taiwan.
Chia-Liang
CL
Lin
Institute of Biochemistry, Microbiology and Immunology, Chung Shan Medical University, Taichung, Taiwan.
Te-Fang
TF
Yang
Institute of Biochemistry, Microbiology and Immunology, Chung Shan Medical University, Taichung, Taiwan. hyhsien@csmu.edu.tw.|Department of Biochemistry, School of Medicine, Chung Shan Medical University, Taichung, Taiwan. hyhsien@csmu.edu.tw.
Yi-Hsien
YH
Hsieh
Physical Therapy Room, Chung Shan Medical University Hospital, Taichung, Taiwan. kunchung@seed.net.tw.|School of Physical Therapy, College of Medical Technology, Chung Shan Medical University, Taichung, Taiwan. kunchung@seed.net.tw.
Kun-Chung
KC
Chen
D000328:Adult; D000368:Aged; D000818:Animals; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D002097:C-Reactive Protein / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle / Q000235:genetics; D059447:Cell Cycle Checkpoints / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology*; D004195:Disease Models, Animal; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000503:physiopathology; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D000683:Serum Amyloid P-Component / Q000235:genetics / Q000378:metabolism*; D000099153:Pentraxins
D002097:C-Reactive Protein; D020781:Matrix Metalloproteinase 1; D020780:Matrix Metalloproteinase 9; D034741:RNA, Small Interfering; D000683:Serum Amyloid P-Component; D000099153:Pentraxins
Gliomas; Invasion; Migration; Pentraxin 3; Proliferation