Efficacy assessment of local doxycycline treatment in periodontal patients using multivariate chemometric approach.
The aim of our study was application of chemometric algorithms for multivariate data analysis in efficacy assessment of the local periodontal treatment with doxycycline (DOX). Treatment efficacy was evaluated by monitoring inflammatory biomarkers in gingival crevicular fluid (GCF) samples and clinical indices before and after the local treatment as well as by determination of DOX concentration in GCF after the local treatment. The experimental values from these determinations were submitted to several chemometric algorithms: principal component analysis (PCA), partial least square discriminant analysis (PLS-DA) and orthogonal projection to latent structures-discriminant analysis (OPLS-DA). The data structure and the mutual relations of the selected variables were thoroughly investigated by PCA. The PLS-DA model identified variables responsible for discrimination of classes of data, before and after DOX treatment. The OPLS-DA model compared the efficacy of the two commonly used medications in periodontal treatment, chlorhexidine (CHX) and DOX, at the same time providing insight in their mechanism of action. The obtained results indicate that application of multivariate chemometric algorithms can be used as a valuable approach for assessment of treatment efficacy.
Institute of Applied Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy, University of Ss. Cyril and Methodius, Mother Theresa 47, 1000 Skopje, Former Yugoslav Republic of Macedonia. Electronic address: lbogdanovska@ff.ukim.edu.mk.
Liljana
L
Bogdanovska
Institute of Applied Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy, University of Ss. Cyril and Methodius, Mother Theresa 47, 1000 Skopje, Former Yugoslav Republic of Macedonia.
Ana
A
Poceva Panovska
Institute of Applied Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy, University of Ss. Cyril and Methodius, Mother Theresa 47, 1000 Skopje, Former Yugoslav Republic of Macedonia.
Natalija
N
Nakov
Institute of Applied Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy, University of Ss. Cyril and Methodius, Mother Theresa 47, 1000 Skopje, Former Yugoslav Republic of Macedonia.
Marija
M
Zafirova
Department of Oral Pathology and Periodontology, Faculty of Dentistry, University of Ss. Cyril and Methodius, Mother Theresa 47, 1000 Skopje, Former Yugoslav Republic of Macedonia.
Mirjana
M
Popovska
Institute of Applied Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy, University of Ss. Cyril and Methodius, Mother Theresa 47, 1000 Skopje, Former Yugoslav Republic of Macedonia.
Aneta
A
Dimitrovska
Institute of Applied Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy, University of Ss. Cyril and Methodius, Mother Theresa 47, 1000 Skopje, Former Yugoslav Republic of Macedonia.
Rumenka
R
Petkovska
D000465:Algorithms; D000469:Alkaline Phosphatase / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000493:pharmacokinetics / Q000494:pharmacology / Q000627:therapeutic use*; D001219:Aspartate Aminotransferases / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D016002:Discriminant Analysis; D004318:Doxycycline / Q000493:pharmacokinetics / Q000494:pharmacology / Q000627:therapeutic use*; D005883:Gingival Crevicular Fluid / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D016018:Least-Squares Analysis; D015999:Multivariate Analysis; D010510:Periodontal Diseases / Q000188:drug therapy / Q000378:metabolism; D025341:Principal Component Analysis; D016896:Treatment Outcome; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000900:Anti-Bacterial Agents; D015415:Biomarkers; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000469:Alkaline Phosphatase; D004318:Doxycycline
Chemometric algorithms; Data analysis; Doxycyline; Periodontal disease; Treatment efficacy