Mulberry ethanol extract attenuates hepatic steatosis and insulin resistance in high-fat diet-fed mice.
Nonalcoholic fatty liver disease is one of the most common complications of obesity. Mulberry is an important source of phytochemicals, such as anthocyanins, polyphenols and flavonoids, which are related to its antioxidant activity. In this study, we developed a hypothesis that mulberry exerted beneficial effects on metabolic disorders and evaluated the influence of the mulberry ethanol extract (MEE) on high-fat diet-induced hepatic steatosis and insulin resistance in mice. Thirty-six male C57BL/6J mice were assigned into 3 groups and fed either a low-fat diet or a high-fat diet with or without supplementation with MEE. Our results showed that administration of MEE reduced diet-induced body weight gain, improved high-fat diet-induced hepatic steatosis and adipose hypertrophy, alleviated insulin resistance, and improved glucose homeostasis. Analysis of hepatic gene expression indicated that MEE treatment changed the expression profile of genes involved in lipid and cholesterol metabolism. In conclusion, the present study demonstrated that MEE supplementation protected mice from high-fat diet-induced obesity, hepatic steatosis, and insulin resistance. Moreover, the protective effects of MEE were associated with the induction of fatty acid oxidation and decreased fatty acid and cholesterol biosynthesis.
College of Biosystems Engineering and Food Science, Zhejiang university, Hangzhou, PR China; Fuli Institute of Food Science, Zhejiang University, Hangzhou, PR China; Zhejiang Key Laboratory for Agro-food Processing, Zhejiang University, Hangzhou, PR China.
Haizhao
H
Song
College of Biosystems Engineering and Food Science, Zhejiang university, Hangzhou, PR China.
Jia
J
Lai
College of Biosystems Engineering and Food Science, Zhejiang university, Hangzhou, PR China.
Qiong
Q
Tang
College of Biosystems Engineering and Food Science, Zhejiang university, Hangzhou, PR China; Fuli Institute of Food Science, Zhejiang University, Hangzhou, PR China; Zhejiang Key Laboratory for Agro-food Processing, Zhejiang University, Hangzhou, PR China. Electronic address: xdzhengzju@yahoo.com.
Xiaodong
X
Zheng
D050154:Adiposity / Q000187:drug effects; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001219:Aspartate Aminotransferases / Q000097:blood; D001786:Blood Glucose / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D018752:Diet, Fat-Restricted; D059305:Diet, High-Fat / Q000009:adverse effects; D000431:Ethanol; D005234:Fatty Liver / Q000188:drug therapy*; D005638:Fruit / Q000737:chemistry*; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D050356:Lipid Metabolism / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D031625:Morus / Q000737:chemistry*; D009478:Neuropeptide Y / Q000097:blood; D064209:Phytochemicals / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D014280:Triglycerides / Q000097:blood; D015430:Weight Gain / Q000187:drug effects
D000975:Antioxidants; D001786:Blood Glucose; D007328:Insulin; D009478:Neuropeptide Y; D064209:Phytochemicals; D010936:Plant Extracts; D014280:Triglycerides; D000431:Ethanol; D002784:Cholesterol; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase
Glucose homeostasis; Hepatic steatosis; Insulin resistance; Mulberry