Data on the identity and myristoylation state of recombinant, purified hippocalcin.
In this data article we report on the purity and post translation modification of bacterially expressed and purified recombinant hippocalcin (HPCA): a member of the neuronal calcium sensor protein family, whose functions are regulated by calcium. MALDI-TOF in source decay (ISD) analysis was used to identify both the myristoylated or non-myristoylated forms of the protein. MALDI-TOF ISD data on the identity of the protein, amino acid sequence and myristoylation efficiency are provided. This data relates to the article "Single-Column Purification of the Tag-free, Recombinant Form of the Neuronal Calcium Sensor Protein, Hippocalcin Expressed in Eschericia coli" [1].
Graduate School of Biomedical Sciences, USA.
Anuradha
A
Krishnan
Graduate School of Biomedical Sciences, USA.
Jeffrey
J
Viviano
Graduate School of Biomedical Sciences, USA.
Yaroslav
Y
Morozov
Graduate School of Biomedical Sciences, USA; School of Osteopathic Medicine Rowan University, Stratford, NJ 08084, USA.
Venkat
V
Venkataraman
Calcium; Hippocalcin; ISD sequencing; MALDI-TOF; Myristoylation; NCS proteins