In Vivo Tumor Angiogenesis Imaging Using Peptide-Based Near-Infrared Fluorescent Probes.
Near-infrared fluorescence (NIRF) imaging is an emerging imaging technique for studying diseases at the molecular level. Optical imaging with a near-infrared emitting fluorophore for targeting tumor angiogenesis offers a noninvasive method for early tumor detection and efficient monitoring of tumor response to anti-angiogenesis therapy. CD13 receptor, a zinc-dependent membrane-bound ectopeptidase, plays important roles in regulating tumor angiogenesis and the growth of new blood vessels. In this chapter, we use CD13 receptor as an example to demonstrate how to construct CD13-specific NGR-containing peptides via bioorthogonal click chemistry for visualizing and quantifying the CD13 receptor expression in vivo by means of NIRF optical imaging.
Molecular Imaging Center, Department of Radiology, Keck School of Medicine, University of Southern California, 2250 Alcazar Street, CSC 103, Los Angeles, CA, 90033-9061, USA.
Rui
R
Huang
Molecular Imaging Center, Department of Radiology, Keck School of Medicine, University of Southern California, 2250 Alcazar Street, CSC 103, Los Angeles, CA, 90033-9061, USA.
Peter S
PS
Conti
Molecular Imaging Center, Department of Radiology, Keck School of Medicine, University of Southern California, 2250 Alcazar Street, CSC 103, Los Angeles, CA, 90033-9061, USA. chenkai@med.usc.edu.
Kai
K
Chen
D000818:Animals; D018826:CD13 Antigens / Q000378:metabolism*; D045744:Cell Line, Tumor; D057930:Click Chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D061986:MCF-7 Cells; D051379:Mice; D057054:Molecular Imaging; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000098:blood supply* / Q000000981:diagnostic imaging / Q000378:metabolism; D009389:Neovascularization, Pathologic / Q000000981:diagnostic imaging* / Q000378:metabolism; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry; D041622:Tomography, Optical / Q000379:methods*
D005456:Fluorescent Dyes; C526428:NGR peptide; D009842:Oligopeptides; D018826:CD13 Antigens
Cancer; Molecular imaging; Near-infrared fluorescence imaging; Peptide-based probes; Tumor angiogenesis