Response of selenium-dependent glutathione peroxidase in the freshwater bivalve Anodonta woodiana exposed to 2,4-dichlorophenol,2,4,6-trichlorophenol and pentachlorophenol.
2,4-dichlorophenol (2,4-DCP), 2,4,6-trichlorophenol (2,4,6-TCP), and pentachlorophenol (PCP) pose a health risk to aquatic organism and humans, and are recognized as persistent priority pollutants. Selenium dependent glutathione peroxidase (Se-GPx) belongs to the family of selenoprotein, which acts mainly as an antioxidant role in the cellular defense system. In the current study, a Se-GPx full length cDNA was cloned from Anodonta woodiana and named as AwSeGPx. It had a characteristic codon at 165TGA167 that corresponds to selenocysteine(Sec) amino acid as U44. The full length cDNA consists of 870 bp, an open reading frame (ORF) of 585 bp encoded a polypeptide of 195 amino in which conserved domain (68LGFPCNQF75) and a glutathione peroxide-1 GPx active site (32GKVILVENVASLUGTT47) were observed. Additionally, the eukaryotic selenocysteine insertion sequence (SECIS) was conserved in the 3'UTR. The AwSeGPx amino acid sequence exhibited a high similarity with that of other Se-GPx. Real-time PCR analysis revealed that AwSeGPx mRNA had a widely distribution, but the highest level was observed in hepatopancreas. AwSeGPx mRNA expression was significantly up-regulated in hepatopancreas, gill and hemocytes after 2,4-DCP, 2,4,6-TCP and PCP exposure. Under similar environment, clams A. woodiana showed a more sensitive to PCP than that of 2,4-DCP and 2,4,6-TCP. These results indicate that AwSeGPx plays a protective role in eliminating oxidative stress derived from 2,4-DCP, 2,4,6-TCP and PCP treatment.
Basal Medicine Institution of Nanyang Medical College, Nanyang 473041, Henan Province, China; State Key Laboratory of Environmental Chemistry and Eco-toxicology, Research Centre for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. Electronic address: xiaxichao8336@163.com.
Xichao
X
Xia
Basal Medicine Institution of Nanyang Medical College, Nanyang 473041, Henan Province, China.
Chunxiu
C
Hua
Basal Medicine Institution of Nanyang Medical College, Nanyang 473041, Henan Province, China.
Shipeng
S
Xue
Basal Medicine Institution of Nanyang Medical College, Nanyang 473041, Henan Province, China.
Bingqin
B
Shi
Basal Medicine Institution of Nanyang Medical College, Nanyang 473041, Henan Province, China.
Gaixia
G
Gui
Basal Medicine Institution of Nanyang Medical College, Nanyang 473041, Henan Province, China.
Dongxian
D
Zhang
Basal Medicine Institution of Nanyang Medical College, Nanyang 473041, Henan Province, China.
Xiying
X
Wang
State Key Laboratory of Environmental Chemistry and Eco-toxicology, Research Centre for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. Electronic address: lhguo@rcees.ac.cn.
Lianghong
L
Guo
D000595:Amino Acid Sequence; D000818:Animals; D049877:Anodonta / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002733:Chlorophenols / Q000633:toxicity; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010416:Pentachlorophenol / Q000633:toxicity; D010802:Phylogeny; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D016415:Sequence Alignment; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D002733:Chlorophenols; D018076:DNA, Complementary; D012333:RNA, Messenger; D014874:Water Pollutants, Chemical; D010416:Pentachlorophenol; D005979:Glutathione Peroxidase; C024564:2,4,6-trichlorophenol; C004762:2,4-dichlorophenol
2,4,6-Trichlorophenol; 2,4-Dichlorophenol; Anodonta woodiana; AwSeGPx; Pentachlorophenol