TLR4 Deficiency Impairs Oligodendrocyte Formation in the Injured Spinal Cord.
Acute oligodendrocyte (OL) death after traumatic spinal cord injury (SCI) is followed by robust neuron-glial antigen 2 (NG2)-positive OL progenitor proliferation and differentiation into new OLs. Inflammatory mediators are prevalent during both phases and can influence the fate of NG2 cells and OLs. Specifically, toll-like receptor (TLR) 4 signaling induces OL genesis in the naive spinal cord, and lack of TLR4 signaling impairs white matter sparing and functional recovery after SCI. Therefore, we hypothesized that TLR4 signaling may regulate oligodendrogenesis after SCI. C3H/HeJ (TLR4-deficient) and control (C3H/HeOuJ) mice received a moderate midthoracic spinal contusion. TLR4-deficient mice showed worse functional recovery and reduced OL numbers compared with controls at 24 h after injury through chronic time points. Acute OL loss was accompanied by reduced ferritin expression, which is regulated by TLR4 and needed for effective iron storage. TLR4-deficient injured spinal cords also displayed features consistent with reduced OL genesis, including reduced NG2 expression, fewer BrdU-positive OLs, altered BMP4 signaling and inhibitor of differentiation 4 (ID4) expression, and delayed myelin phagocytosis. Expression of several factors, including IGF-1, FGF2, IL-1β, and PDGF-A, was altered in TLR4-deficient injured spinal cords compared with wild types. Together, these data show that TLR4 signaling after SCI is important for OL lineage cell sparing and replacement, as well as in regulating cytokine and growth factor expression. These results highlight new roles for TLR4 in endogenous SCI repair and emphasize that altering the function of a single immune-related receptor can dramatically change the reparative responses of multiple cellular constituents in the injured CNS milieu.



Myelinating cells of the CNS [oligodendrocytes (OLs)] are killed for several weeks after traumatic spinal cord injury (SCI), but they are replaced by resident progenitor cells. How the concurrent inflammatory signaling affects this endogenous reparative response is unclear. Here, we provide evidence that immune receptor toll-like receptor 4 (TLR4) supports OL lineage cell sparing, long-term OL and OL progenitor replacement, and chronic functional recovery. We show that TLR4 signaling is essential for acute iron storage, regulating cytokine and growth factor expression, and efficient myelin debris clearance, all of which influence OL replacement. Importantly, the current study reveals that a single immune receptor is essential for repair responses after SCI, and the potential mechanisms of this beneficial effect likely change over time after injury.
Neuroscience Graduate Program, Center for Brain and Spinal Cord Repair, and.
Jamie S
0000-0002-3073-6516
JS
Church
Center for Brain and Spinal Cord Repair, and Department of Neuroscience, Ohio State University, Columbus, Ohio 43210.
Kristina A
0000-0002-1652-0882
KA
Kigerl
Center for Brain and Spinal Cord Repair, and Department of Neuroscience, Ohio State University, Columbus, Ohio 43210.
Jessica K
0000-0002-1210-3184
JK
Lerch
Center for Brain and Spinal Cord Repair, and Department of Neuroscience, Ohio State University, Columbus, Ohio 43210.
Phillip G
0000-0003-1329-7395
PG
Popovich
Center for Brain and Spinal Cord Repair, and Department of Neuroscience, Ohio State University, Columbus, Ohio 43210 Dana.McTigue@osumc.edu.
Dana M
0000-0001-7066-9701
DM
McTigue
D000818:Animals; D001369:Axons / Q000473:pathology; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation / Q000235:genetics; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005106:Exploratory Behavior / Q000502:physiology; D005346:Fibroblast Growth Factors / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics*; D008264:Macrophages / Q000502:physiology; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008822:Mice, Transgenic; D009416:Nerve Regeneration / Q000235:genetics* / Q000502:physiology; D009836:Oligodendroglia / Q000502:physiology*; D010587:Phagocytosis / Q000235:genetics; D020127:Recovery of Function / Q000235:genetics / Q000502:physiology; D013119:Spinal Cord Injuries / Q000473:pathology* / Q000503:physiopathology; D051197:Toll-Like Receptor 4 / Q000172:deficiency* / Q000235:genetics
C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; D005346:Fibroblast Growth Factors
growth factor; inflammation; macrophage; microglia; myelin; phagocytosis