Detection of the plasmid-mediated colistin-resistance gene mcr-1 in clinical isolates and stool specimens obtained from hospitalized patients using a newly developed real-time PCR assay.
Department of Medical Microbiology, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands r.h.t.nijhuis@lumc.nl.
R H T
RH
Nijhuis
Department of Bacteriology and Epidemiology, Central Veterinary Institute (CVI) of Wageningen UR, PO Box 65, 8200 AB Lelystad, The Netherlands.
K T
KT
Veldman
Department of Medical Microbiology, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands.
J
J
Schelfaut
Department of Bacteriology and Epidemiology, Central Veterinary Institute (CVI) of Wageningen UR, PO Box 65, 8200 AB Lelystad, The Netherlands.
A
A
Van Essen-Zandbergen
Department of Medical Microbiology, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands.
E
E
Wessels
Department of Medical Microbiology, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands.
E C J
EC
Claas
Department of Medical Microbiology, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands.
J
J
Gooskens
D003091:Colistin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000235:genetics*; D005243:Feces / Q000382:microbiology; D005798:Genes, Bacterial; D006760:Hospitalization; D006801:Humans; D058885:Multilocus Sequence Typing; D009426:Netherlands / Q000453:epidemiology; D010957:Plasmids / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D062606:Tertiary Care Centers
D029968:Escherichia coli Proteins; C000614037:MCR-1 protein, E coli; D003091:Colistin