LC-MS analysis of Hep-2 and Hek-293 cell lines treated with Brazilian red propolis reveals differences in protein expression.
OBJECTIVE
Red propolis, an exclusive variety of propolis found in the northeast of Brazil has shown to present antitumour activity, among several other biological properties. This article aimed to help to evaluate the underlying molecular mechanisms of the potential anticancer effects of red propolis on tumour, Hep-2, and non-tumour cells, Hek-293.


METHODS
Differentially expressed proteins in human cell lines were identified through label-free quantitative MS-based proteomic platform, and cells were stained with Giemsa to show morphological changes.


RESULTS
A total of 1336 and 773 proteins were identified for Hep-2 and Hek-293, respectively. Among the proteins here identified, 16 were regulated in the Hep-2 cell line and 04 proteins in the Hek-293 line. Over a total of 2000 proteins were identified under MS analysis, and approximately 1% presented differential expression patterns. The GO annotation using Protein Analysis THrough Evolutionary Relationships classification system revealed predominant molecular function of catalytic activity, and among the biological processes, the most prominent was associated to cell metabolism.


CONCLUSIONS
The proteomic profile here presented should help to elucidate further molecular mechanisms involved in inhibition of cancer cell proliferation by red propolis, which remain unclear to date.
Laboratory of Genomics, Proteomics and DNA Repair, Biotechnology Institute, University of Caxias do Sul, Caxias do Sul, RS, Brazil.
Caroline O
CO
da Silva Frozza
Laboratory of Genomics, Proteomics and DNA Repair, Biotechnology Institute, University of Caxias do Sul, Caxias do Sul, RS, Brazil.
Emyle
E
da Silva Brum
Bruker Daltonics, Billerica, MA, USA.
Anjali
A
Alving
Laboratory of Natural and Synthetic Products, Biotechnology Institute, University of Caxias do Sul, Caxias do Sul, RS, Brazil.
Sidnei
S
Moura
Laboratory of Genomics, Proteomics and DNA Repair, Biotechnology Institute, University of Caxias do Sul, Caxias do Sul, RS, Brazil.
João A P
JA
Henriques
Laboratory of Genomics, Proteomics and DNA Repair, Biotechnology Institute, University of Caxias do Sul, Caxias do Sul, RS, Brazil.
Mariana
M
Roesch-Ely
D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D056705:Apitherapy; D001938:Brazil; D045744:Cell Line, Tumor; D002853:Chromatography, Liquid; D057809:HEK293 Cells; D006801:Humans; D013058:Mass Spectrometry; D009369:Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D011429:Propolis / Q000494:pharmacology* / Q000627:therapeutic use; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics
D000970:Antineoplastic Agents; D020543:Proteome; D011429:Propolis
cancer; proteomics; red propolis