Targeted chitosan-based bionanocomposites for controlled oral mucosal delivery of chlorhexidine.
The purpose of this study was to develop sustained release systems based on chitosan (CS) and montmorillonite (MMT) for chlorhexidine (CLX). Nanocomposites were prepared by ion-exchange. CLX systems were characterized by X-ray powder diffraction (XRD), thermal analysis (TGA), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and X-ray fluorescence analysis (XRF). The mucoadhesion properties of CLX nanocomposites were evaluated by SEM. The release behavior of these systems was also studied by the dialysis technique. The antibacterial activity was investigated in vitro by the disk diffusion test. Results showed long-term sustained release of CLX from the hybrid carriers without initial burst release. The release profiles of CLX from the carriers suggested the diffusion through a swollen matrix and water filled pores as the controlled drug release mechanism. The CLX hybrid nanosystem containing the positively-charged chitosan exhibited good mucoadhesion properties maintaining the CLX antimicrobial properties.
Facultad de Ciencias Químicas, UNC-CONICET-UNITEFA, Ciudad Universitaria, X5000-HUA, Córdoba, Argentina.
Renée
R
Onnainty
Dipartimento di Scienza Applicata e Tecnologia, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Torino, Italy.
Barbara
B
Onida
Facultad de Ciencias Químicas, UNC-CONICET-UNITEFA, Ciudad Universitaria, X5000-HUA, Córdoba, Argentina.
Paulina
P
Páez
Facultad de Ciencias Químicas, UNC-CONICET-UNITEFA, Ciudad Universitaria, X5000-HUA, Córdoba, Argentina.
Marcela
M
Longhi
Dipartimento di Scienza Applicata e Tecnologia, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Torino, Italy.
Antonello
A
Barresi
Facultad de Ciencias Químicas, UNC-CONICET-UNITEFA, Ciudad Universitaria, X5000-HUA, Córdoba, Argentina. Electronic address: glagra@fcq.unc.edu.ar.
Gladys
G
Granero
D000284:Administration, Oral; D001546:Bentonite / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D002710:Chlorhexidine / Q000008:administration & dosage* / Q000737:chemistry*; D003692:Delayed-Action Preparations / Q000737:chemistry*; D004058:Diffusion; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods; D008855:Microscopy, Electron, Scanning / Q000379:methods; D009061:Mouth Mucosa / Q000378:metabolism*; D053761:Nanocomposites / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013818:Thermogravimetry; D014961:X-Ray Diffraction / Q000379:methods
D001672:Biocompatible Materials; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D001546:Bentonite; D048271:Chitosan; D002710:Chlorhexidine
Antibacterial activity; Chitosan; Chlorhexidine; Drug delivery systems; Mucoadhesion; Nanocomposites