CBX7 deficiency plays a positive role in dentin and alveolar bone development.
To clarify the role of CBX7 deficiency in dentin and alveolar bone development, the dental and mandibular phenotypes of homozygous CBX7-knockout (CBX7(-/-)) mice were compared with their wild-type (WT) counterparts at 3 weeks age. In contrast to WT littermates, dental volume and dentin sialoprotein-positive area were significantly increased, whereas the area ratio of predentin to dentin was decreased markedly in CBX7(-/-) mice. Mineral density, cortical thickness, alveolar bone volume, type I collagen and osterix-immunopositive area, osteoblast number and activity, protein expression and mRNA level of Runt-related transcription factor 2 (Runx2), alkaline phosphatase, osteocalcin, osteopontin and bone morphogenetic protein 2 (BMP2) were all remarkably increased, while osteoclast number and activity, and mRNA expression ratio of NF-κB ligand (RANKL) to osteoprotegerin (opg) were all decreased significantly in the alveolar bone of CBX7(-/-) mice compared with their WT counterparts. Moreover, proliferating cell nuclear antigen (PCNA)-positive cells were found more in Hertwig' s epithelial root sheath of CBX7(-/-) mice, and their protein level of cyclin E1, cyclin-dependent kinase 2 (CDK2) were correspondingly increased in contrast to WT mice. Taken together, these results of this study suggest that CBX7 deficiency plays a positive role in dentin and alveolar bone formation.
Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, NO.136, Hanzhong Road, Nanjing, 210029, People's Republic of China.
Zhixuan
Z
Zhou
Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, NO.136, Hanzhong Road, Nanjing, 210029, People's Republic of China.
Ying
Y
Yin
Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, NO.136, Hanzhong Road, Nanjing, 210029, People's Republic of China.
Fei
F
Jiang
Department of Stomatology and Center for Evidence-Based Medicine and Clinical Research, Taihe Hospital, Shiyan, Hubei, People's Republic of China.
Yuming
Y
Niu
Zhenjiang Stomatological Hospital, Zhenjiang, Jiangsu, People's Republic of China.
Shujian
S
Wan
Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, NO.136, Hanzhong Road, Nanjing, 210029, People's Republic of China. cn_njmu@yahoo.com.|Department of Dental Implant, Affiliated Hospital of Stomatology, Nanjing, 210029, People's Republic of China. cn_njmu@yahoo.com.
Ning
http://orcid.org/0000-0003-2904-0199
N
Chen
Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, NO.136, Hanzhong Road, Nanjing, 210029, People's Republic of China. mingshen85@yahoo.com.|Department of Dental Implant, Affiliated Hospital of Stomatology, Nanjing, 210029, People's Republic of China. mingshen85@yahoo.com.
Ming
M
Shen
D000818:Animals; D015415:Biomarkers; D001846:Bone Development / Q000235:genetics*; D002453:Cell Cycle / Q000235:genetics; D049109:Cell Proliferation; D003804:Dentin / Q000378:metabolism*; D008334:Mandible / Q000000981:diagnostic imaging / Q000254:growth & development / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D023421:Models, Animal; D009805:Odontogenesis / Q000235:genetics; D010006:Osteoblasts / Q000378:metabolism; D010010:Osteoclasts / Q000378:metabolism; D010012:Osteogenesis / Q000235:genetics*; D063150:Polycomb Repressive Complex 1 / Q000172:deficiency*; D014070:Tooth / Q000254:growth & development / Q000378:metabolism; D055114:X-Ray Microtomography
D015415:Biomarkers; C519505:Cbx7 protein, mouse; D063150:Polycomb Repressive Complex 1
CBX7; Development; Mandible; Mice; Tooth