Mangiferin inhibits macrophage classical activation via downregulating interferon regulatory factor 5 expression.
Mangiferin is a natural polyphenol and the predominant effective component of Mangifera indica Linn. leaves. For hundreds of years, Mangifera indica Linn. leaf has been used as an ingredient in numerous traditional Chinese medicine preparations for the treatment of bronchitis. However, the pharmacological mechanism of mangiferin in the treatment of bronchitis remains to be elucidated. Macrophage classical activation is important role in the process of bronchial airway inflammation, and interferon regulatory factor 5 (IRF5) has been identified as a key regulatory factor for macrophage classical activation. The present study used the THP‑1 human monocyte cell line to investigate whether mangiferin inhibits macrophage classical activation via suppressing IRF5 expression in vitro. THP‑1 cells were differentiated to macrophages by phorbol 12‑myristate 13‑acetate. Macrophages were polarized to M1 macrophages following stimulation with lipopolysaccharide (LPS)/interferon‑γ (IFN‑γ). Flow cytometric analysis was conducted to detect the M1 macrophages. Reverse transcription‑quantitative polymerase chain reaction was used to investigate cellular IRF5 gene expression. Levels of proinflammatory cytokines and IRF5 were assessed following cell culture and cellular homogenization using enzyme‑linked immunosorbent assay. IRF5 protein and nuclei co‑localization was performed in macrophages with laser scanning confocal microscope immunofluorescence analysis. The results of the present study demonstrated that mangiferin significantly inhibits LPS/IFN‑γ stimulation‑induced classical activation of macrophages in vitro and markedly decreases proinflammatory cytokine release. In addition, cellular IRF5 expression was markedly downregulated. These results suggest that the inhibitory effect of mangiferin on classical activation of macrophages may be exerted via downregulation of cellular IRF5 expression levels.
Guangxi Scientific Experimental Center of Traditional Chinese Medicine, Guangxi University of Chinese Medicine, Nanning, Guangxi 530001, P.R. China.
Zhiquan
Z
Wei
Laboratory of Basis and Application Research of Zhuang Medicine Formulas, Zhuang Medicine College, Guangxi University of Chinese Medicine, Nanning, Guangxi 530001, P.R. China.
Li
L
Yan
Guangxi Key Laboratory of Pharmacodynamics Studies of Traditional Chinese Medicine, Nanning, Guangxi 530001, P.R. China.
Yixin
Y
Chen
Department of Pharmacy, Ruikang Affiliated Hospital, Guangxi University of Chinese Medicine, Nanning, Guangxi 530012, P.R. China.
Chuanhong
C
Bao
Dana‑Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA.
Jing
J
Deng
Guangxi Key Laboratory of Pharmacodynamics Studies of Traditional Chinese Medicine, Nanning, Guangxi 530001, P.R. China.
Jiagang
J
Deng
D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D016130:Immunophenotyping; D018836:Inflammation Mediators / Q000378:metabolism; D050835:Interferon Regulatory Factors / Q000235:genetics*; D008262:Macrophage Activation / Q000187:drug effects* / Q000502:physiology*; D008264:Macrophages / Q000187:drug effects* / Q000502:physiology*; D010641:Phenotype; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D021381:Protein Transport; D044004:Xanthones / Q000737:chemistry / Q000494:pharmacology*
D016207:Cytokines; C431713:IRF5 protein, human; D018836:Inflammation Mediators; D050835:Interferon Regulatory Factors; D010936:Plant Extracts; D044004:Xanthones; C013592:mangiferin