Laterosporulin10: a novel defensin like Class IId bacteriocin from Brevibacillus sp. strain SKDU10 with inhibitory activity against microbial pathogens.
Bacteriocins are antimicrobial peptides (AMPs) produced by bacteria to acquire survival benefits during competitive inter- and intra-species interactions in complex ecosystems. In this study, an AMP-producing soil bacterial strain designated SKDU10 was isolated and identified as a member of the genus Brevibacillus. The AMP produced by strain SKDU10 identified as a class IId bacteriocin with 57.6 % homology to laterosporulin, a defensin-like class IId bacteriocin. However, substantial differences were observed in the antimicrobial activity spectrum of this bacteriocin named laterosporulin10 when compared to laterosporulin. Laterosporulin10 effectively inhibited the growth of Staphylococcus aureus and Mycobacterium tuberculosis (Mtb H37Rv) with LD50 values of 4.0 µM and 0.5 µM, respectively. Furthermore, laterosporulin10 inhibited the growth of Mtb H37Rv strain at about 20 times lower MIC value compared to S. aureus MTCC 1430 or M. smegmatis MC2 155 in vitro and ex vivo. Electron micrographs along with membrane permeabilization studies using FACS analysis revealed that laterosporulin10 is a membrane-permeabilizing peptide. Interestingly, laterosporulin10 was able to efficiently kill Mtb H37Rv strain residing inside the macrophages and did not show haemolysis up to 40 µM concentration.
CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh-160036, India.
Piyush
P
Baindara
CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh-160036, India.
Nisha
N
Singh
CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh-160036, India.
Manish
M
Ranjan
CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh-160036, India.
Nayudu
N
Nallabelli
CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh-160036, India.
Vasvi
V
Chaudhry
CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh-160036, India.
Geeta Lal
GL
Pathania
CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh-160036, India.
Nidhi
N
Sharma
CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh-160036, India.
Ashwani
A
Kumar
CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh-160036, India.
Prabhu B
PB
Patil
CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh-160036, India.
Suresh
S
Korpole
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology*; D001430:Bacteriocins / Q000378:metabolism / Q000494:pharmacology*; D001483:Base Sequence; D058675:Brevibacillus / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D023082:Defensins / Q000378:metabolism / Q000494:pharmacology*; D016680:Genome, Bacterial / Q000235:genetics; D008826:Microbial Sensitivity Tests; D020102:Mycobacterium smegmatis / Q000187:drug effects* / Q000254:growth & development; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000254:growth & development; D017422:Sequence Analysis, DNA; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D004269:DNA, Bacterial; D023082:Defensins; C000596376:laterosporulin