Establishment of local searching methods for orbitrap-based high throughput metabolomics analysis.
Our method aims to establish local endogenous metabolite databases economically without purchasing chemical standards, giving strong bases for following orbitrap based high throughput untargeted metabolomics analysis. A new approach here is introduced to construct metabolite databases on the base of biological sample analysis and mathematic extrapolation. Building local metabolite databases traditionally requires expensive chemical standards, which is barely affordable for most research labs. As a result, most labs working on metabolomics analysis have to refer public libraries, which is time consuming and limited for high throughput analysis. Using this strategy, a high throughput orbitrap based metabolomics platform can be established at almost no cost within a couple of months. It enables to facilitate the application of high throughput metabolomics analysis to identify disease-related biomarkers or investigate biological functions using orbitrap.
School of Life Sciences, Tsinghua University, Beijing 100084, China.
Haiping
H
Tang
Technology Center for Protein Sciences, Tsinghua University, Beijing 100084, China.
Xueying
X
Wang
Technology Center for Protein Sciences, Tsinghua University, Beijing 100084, China.
Lina
L
Xu
Thermo Fisher Scientific (China), Beijing 100007, China.
Xiaorong
X
Ran
Thermo Fisher Scientific (China), Beijing 100007, China.
Xiangjun
X
Li
School of Pharmaceutical Sciences, Tsinghua University, Beijing 100084, China.
Ligong
L
Chen
School of Pharmaceutical Sciences, Tsinghua University, Beijing 100084, China.
Xinbin
X
Zhao
School of Life Sciences, Tsinghua University, Beijing 100084, China. Electronic address: dht@mail.tsinghua.edu.cn.
Haiteng
H
Deng
School of Life Sciences, Tsinghua University, Beijing 100084, China; Technology Center for Protein Sciences, Tsinghua University, Beijing 100084, China. Electronic address: xiaohui2013@mail.tsinghua.edu.cn.
Xiaohui
X
Liu
ID-based metabolomics analysis; In-house endogenous databases; LC-MS/MS; Orbitrap