Disturbance of Antioxidant Enzymes and Purine Metabolism in the Ejaculate of Men Living in Disadvantaged Areas of Kyzylorda Region.
OBJECTIVE
Objective of the study was to evaluate the state of the main indicators of antioxidant status and enzymes of purine metabolism in the germ cells of men living in the zone of ecological catastrophe Aral Sea region.


METHODS
The criterion for inclusion is the stay of an adult in the Aral Sea area is not less than 5 years, employment in occupations with no more than 2 hazard class. Determination of the activity of adenosine deaminase (ADA) was conducted in semen by the method of Nemechek et al., 1993. Determination of the activity of catalase (CAT) was performed according by the method of Korolyuk et al., 1988.


RESULTS
Results of the study indicate a change in the activity of catalase and adenosine deaminase, due to increased levels of oxidative stress and the development of the pathological process.


CONCLUSIONS
According to the results of study, it was put the influence of negative factors of the Aral Sea region in men's sperm of reproductive age gives to disability free-radical processes, that proves changing of ferments of ant oxidative protection Catalase and adenosine deaminase (ADA). This disturbance in men's sperm of reproductive age leading to increased level of oxidative stress and impaired activity of antioxidant enzymes and purine metabolism, responsible for the abnormal transmembrane and intracellular processes, reflecting the degree of imbalance of enzymes.
Karaganda State Medical University, Department of Obstetrics and Gynecology, Molecular Biology and Medical Genetics, Gogol 40, Karaganda 1000008, Kazakhstan.
Valentihna N
VN
Kislitskaya
Karaganda State Medical University, Department of Obstetrics and Gynecology, Molecular Biology and Medical Genetics, Gogol 40, Karaganda 1000008, Kazakhstan.
Zhandos D
ZD
Kenzhin
Karaganda State Medical University, Department of Obstetrics and Gynecology, Molecular Biology and Medical Genetics, Gogol 40, Karaganda 1000008, Kazakhstan.
Berikbay Zh
BZh
Kultanov
Karaganda State Medical University, Department of Obstetrics and Gynecology, Molecular Biology and Medical Genetics, Gogol 40, Karaganda 1000008, Kazakhstan.
Raushan S
RS
Dosmagambetova
Karaganda State Medical University, Department of Obstetrics and Gynecology, Molecular Biology and Medical Genetics, Gogol 40, Karaganda 1000008, Kazakhstan.
Anar A
AA
Turmuhambetova
Environmental disaster; adenosine deaminase; antioxidant status; catalase; heavy metals; oxidative stress; pesticides