Production of a Novel Extracellular Polysaccharide by a Bacillus Strain Isolated from Soil.
A bacterium that was isolated from soil and identified as Bacillus circulans was found to produce a highly viscous extracellular polysaccharide when it was grown aerobically in a medium containing glucose as a sole source of carbon. The product was characterized by TLC and GC analyses as a novel heteropolysaccharide consisted of rhamnose, mannose, galactose, and mannuronic acid as sugar components. A maximal yield of polysaccharide reached about 2 g/liter by jar-fermentor culture at 30°C for 48 hr with a medium containing 1% glucose, 0.05% asparagine, 0.005% yeast extract, and small amounts of inorganic salts. Some culture conditions for the production of polysaccharide were investigated with flask culture; an optimal production was attained with a medium containing 0.1-1 % glucose and 0.01-0.05% asparagine, pH 7-8, at 30°C under aerobic conditions.
a Department of Food Science and Nutrition , Nara Women's University , Nara 630 , Japan.
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Kawai
a Department of Food Science and Nutrition , Nara Women's University , Nara 630 , Japan.
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Isobe
a Department of Food Science and Nutrition , Nara Women's University , Nara 630 , Japan.
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Horibe
a Department of Food Science and Nutrition , Nara Women's University , Nara 630 , Japan.
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Tokuda
a Department of Food Science and Nutrition , Nara Women's University , Nara 630 , Japan.
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Tokuno
a Department of Food Science and Nutrition , Nara Women's University , Nara 630 , Japan.
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Endo
a Department of Food Science and Nutrition , Nara Women's University , Nara 630 , Japan.|b Department of Biology , Kobe University of Commerce , Kobe 655 , Japan.
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Kawai