Improvement on light penetrability and microalgae biomass production by periodically pre-harvesting Chlorella vulgaris cells with culture medium recycling.
To improve light penetrability and biomass production in batch cultivation, a cultivation mode that periodically pre-harvesting partial microalgae cells from suspension with culture medium recycling was proposed. By daily pre-harvesting 30% microalgae cells from the suspension, the average light intensity in the photobioreactor (PBR) was enhanced by 27.05-122.06%, resulting in a 46.48% increase in total biomass production than that cultivated in batch cultivation without pre-harvesting under an incident light intensity of 160μmolm(-2)s(-1). Compared with the semi-continuous cultivation with 30% microalgae suspension daily replaced with equivalent volume of fresh medium, nutrients and water input was reduced by 60% in the proposed cultivation mode but with slightly decrease (12.82%) in biomass production. No additional nutrient was replenished when culture medium recycling. Furthermore, higher pre-harvesting ratios (40%, 60%) and lower pre-harvesting frequencies (every 2, 2.5days) were not advantageous for the pre-harvesting cultivation mode.
Key Laboratory of Low-grade Energy Utilization Technologies and Systems, Chongqing University, Ministry of Education, Chongqing 400044, China; Institute of Engineering Thermophysics, Chongqing University, Chongqing 400044, China.
Yun
Y
Huang
Key Laboratory of Low-grade Energy Utilization Technologies and Systems, Chongqing University, Ministry of Education, Chongqing 400044, China; Institute of Engineering Thermophysics, Chongqing University, Chongqing 400044, China.
Yahui
Y
Sun
Key Laboratory of Low-grade Energy Utilization Technologies and Systems, Chongqing University, Ministry of Education, Chongqing 400044, China; Institute of Engineering Thermophysics, Chongqing University, Chongqing 400044, China. Electronic address: lqzx@cqu.edu.cn.
Qiang
Q
Liao
Key Laboratory of Low-grade Energy Utilization Technologies and Systems, Chongqing University, Ministry of Education, Chongqing 400044, China; Institute of Engineering Thermophysics, Chongqing University, Chongqing 400044, China.
Qian
Q
Fu
Key Laboratory of Low-grade Energy Utilization Technologies and Systems, Chongqing University, Ministry of Education, Chongqing 400044, China; Institute of Engineering Thermophysics, Chongqing University, Chongqing 400044, China.
Ao
A
Xia
Key Laboratory of Low-grade Energy Utilization Technologies and Systems, Chongqing University, Ministry of Education, Chongqing 400044, China; Institute of Engineering Thermophysics, Chongqing University, Chongqing 400044, China.
Xun
X
Zhu
D061249:Batch Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D018533:Biomass; D048408:Chlorella vulgaris / Q000254:growth & development* / Q000378:metabolism; D003470:Culture Media; D008027:Light; D058086:Microalgae / Q000254:growth & development* / Q000378:metabolism; D058433:Photobioreactors; D059027:Recycling
D003470:Culture Media
Light attenuation; Light penetrability; Microalgae; Periodically pre-harvesting; Photobioreactor