Identification of a nonsense mutation in APAF1 that is likely causal for a decrease in reproductive efficiency in Holstein dairy cattle.
The HH1 haplotype on chromosome 5 is associated with a reduced conception rate and a deficit of homozygotes at the population level in Holstein cattle. The source HH1 haplotype was traced to the bull Pawnee Farm Arlinda Chief (Chief), who was born in 1962 and has sired more than 16,000 daughters. We identified a nonsense mutation in APAF1 (apoptotic protease activating factor 1;APAF1 p.Q579X) within HH1 using whole-genome resequencing of Chief and 3 of his sons. This mutation is predicted to truncate 670 AA (53.7%) of the encoded APAF1 protein that contains a WD40 domain critical to protein-protein interactions. Initial screening revealed no homozygous individuals for the mutation in 758 animals previously genotyped, whereas all 497 HH1 carriers possessed 1 copy of the mutant allele. Subsequent commercial genotyping of 246,773 Holsteins revealed 5,299 APAF1 heterozygotes and zero homozygotes for the mutation. The causative role of this mutation is also supported by functional data in mice that have demonstrated Apaf1 to be an essential molecule in the cytochrome-c-mediated apoptotic cascade and directly implicated in developmental and neurodegenerative disorders. In addition, most Apaf1 homozygous knockouts die by day 16.5 of development. We thus propose that the APAF1 p.Q579X nonsense mutation is the functional equivalent of the Apaf1 knockout. This mutation has caused an estimated 525,000 spontaneous abortions worldwide over the past 35 years, accounting for approximately $420 million in losses. With the mutation identified, selection against the deleterious allele in breeding schemes has aided in eliminating this defect from the population, reducing carrier frequency from 8% in past decades to 2% in 2015.
Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana 61801; Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana 61801.
Heather A
HA
Adams
Animal Improvement Programs Laboratory, Agricultural Research Service, USDA, Beltsville, MD 20705.
Tad S
TS
Sonstegard
Animal Improvement Programs Laboratory, Agricultural Research Service, USDA, Beltsville, MD 20705.
Paul M
PM
VanRaden
Animal Improvement Programs Laboratory, Agricultural Research Service, USDA, Beltsville, MD 20705.
Daniel J
DJ
Null
Animal Improvement Programs Laboratory, Agricultural Research Service, USDA, Beltsville, MD 20705.
Curt P
CP
Van Tassell
Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana 61801.
Denis M
DM
Larkin
Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana 61801; Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana 61801; Department of Evolution and Ecology and the UC Davis Genome Center, University of California, Davis, Davis 95616. Electronic address: lewin@ucdavis.edu.
Harris A
HA
Lewin
D000034:Abortion, Veterinary; D000818:Animals; D053477:Apoptotic Protease-Activating Factor 1 / Q000235:genetics*; D001947:Breeding; D002417:Cattle; D018389:Codon, Nonsense; D005838:Genotype; D008297:Male
D053477:Apoptotic Protease-Activating Factor 1; D018389:Codon, Nonsense
APAF1; dairy cattle; nonsense mutation; resequencing