2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside suppresses human colorectal cancer cell metastasis through inhibiting NF-κB activation.
2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside (THSG), a major component of Polygonum multiflorum Thunb (He-Shou-Wu), has been reported to exhibit antioxidant and anti-inflammatory effects. However, its anti-metastatic effect against colorectal cancer is still unclear. In this study, cell migration, invasion and adhesion abilities as well as metastasis-associated protein and NF-κB pathway signaling factor expression were analyzed after treating HT-29 cells with THSG. According to the results, the migration and invasiveness of HT-29 cells were reduced after treatment with 5 or 10 mM THSG (p<0.05). Additionally, the levels of matrix metalloproteinase-2 (MMP-2) and phosphorylated VE-cadherin in HT-29 cells were reduced and the transepithelial electrical resistance (TEER) of EA.hy926 endothelial cell monolayers was increased after incubation in THSG for 24 h (p<0.05). Cell adhesion ability and the E-selectin and intercellular adhesion molecule-1 (ICAM-1) protein levels were reduced when EA.hy926 endothelial cells were treated with THSG (p<0.05). In addition, the cytoplasmic phosphorylation of IκB, the nuclear p65 level and the DNA-binding activity of NF-κB were reduced after treating HT-29 or EA.hy926 cells with 5 or 10 mM THSG (p<0.05). These results suggest that THSG inhibits HT-29 cell metastasis by suppressing cell migration, invasion and adhesion. Furthermore, THSG inhibits metastasis-associated protein expression by suppressing NF-κB pathway activation.
Department of Pharmacy, Yuan's General Hospital, Kaohsiung 802, Taiwan, R.O.C.
Chien-Liang
CL
Lin
Department of Seafood Science, National Kaohsiung Marine University, Kaohsiung 811, Taiwan, R.O.C.
Shu-Ling
SL
Hsieh
Department of Radiology and Nuclear Medicine, Yuan's General Hospital, Kaohsiung 802, Taiwan, R.O.C.
Wan
W
Leung
Department of Dentistry, National Taiwan University Hospital, Taipei 100, Taiwan, R.O.C.
Jiiang-Huei
JH
Jeng
Department of Health-Business Administration, School of Nursing, Fooyin University, Kaohsiung 831, Taiwan, R.O.C.
Guan-Cheng
GC
Huang
Department of Seafood Science, National Kaohsiung Marine University, Kaohsiung 811, Taiwan, R.O.C.
Chining-Ting
CT
Lee
Department of Nutrition and Health Sciences, Chang Jung Christian University, Tainan 711, Taiwan, R.O.C.
Chih-Chung
CC
Wu
D002465:Cell Movement / Q000187:drug effects; D015179:Colorectal Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D005960:Glucosides / Q000494:pharmacology*; D019073:HT29 Cells; D006801:Humans; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000378:metabolism; D009362:Neoplasm Metastasis; D015398:Signal Transduction / Q000187:drug effects; D013267:Stilbenes / Q000494:pharmacology*
D005960:Glucosides; D016328:NF-kappa B; D013267:Stilbenes; C093026:2,3,5,4'-tetrahydroxystilbene 2-O-glucopyranoside