Evaluation of the abbot Architect(™) epstein-barr virus viral capsid antigen IgM, viral capsid antigen IgG and nuclear antigen IgG assays in a pediatric and adult population.
The detection of antibodies against Epstein-Barr viral capsid (VCA) and nuclear (EBNA) antigens is routinely performed with different commercially available immunoassays.



In this study, we evaluated the concordance and performance of the Architect(™) chemiluminescent microparticle immunoassays (CMIAs) using Captia(™) enzyme linked immunosorbent assays (ELISA) for VCA IgM, and standard immunofluorescence (IF) assays for VCA IgG and EBNA IgG as comparative techniques.



Sera were selected from a heterogeneous population including pediatric and adult patients.



Concordance between CMIAs and comparative assays was high with total agreement percentages of 84,1% (95% CI: 77.8-88.9) for VCA IgM, 90,6% (95% CI: 84.2-94.7) for EBNA IgG and 98,0% (95% CI: 93.9-99.6) for VCA IgG. Moreover, kappa statistic values showed good to excellent correlation with values of 0.68 (95% CI: 0.57-0.79) for VCA IgM, 0.73 (95% CI: 0.58-0.87) for EBNA IgG and 0.95 (95% CI: 0.89-1.00) for VCA IgG. A correlation was observed between positivity levels on CMIAs and semi-quantitative fluorescence intensity on IF for VCA IgG and EBNA IgG assays. With regard to an accepted gold standard IF assays, CMIA was 98,1% (95% CI: 93.3-99.8) sensitive and 97,4% (95% CI: 86.5-99.9) specific for the detection of VCA IgG. For the detection of EBNA IgG, it was 92,2% (95% CI: 85.1-96.6) sensitive and 84,6% (95% CI: 65.1-95.6) specific.



In summary, we demonstrated that the CMIA EBV antibody detection panel has high performance and high concordance with other commercially available immunoassays.
Centre Hospitalier Universitaire Sainte-Justine, 3175 Chemin de la Côte Sainte-Catherine, Montreal, Quebec H3T 1C4, Canada; University of Montreal, 2900 Boulevard Edouard-Monpetit, Montreal, Quebec H3T 1J4, Canada. Electronic address: Simon.Grandjean.Lapierre@Umontreal.ca.
Simon
S
Grandjean Lapierre
Centre Hospitalier Universitaire Sainte-Justine, 3175 Chemin de la Côte Sainte-Catherine, Montreal, Quebec H3T 1C4, Canada; University of Montreal, 2900 Boulevard Edouard-Monpetit, Montreal, Quebec H3T 1J4, Canada.
Emilie
E
Vallières
Centre Hospitalier Universitaire Sainte-Justine, 3175 Chemin de la Côte Sainte-Catherine, Montreal, Quebec H3T 1C4, Canada.
Leila
L
Rabaamad
Centre Hospitalier Universitaire Sainte-Justine, 3175 Chemin de la Côte Sainte-Catherine, Montreal, Quebec H3T 1C4, Canada.
Manon
M
Labrecque
Centre Hospitalier Universitaire Sainte-Justine, 3175 Chemin de la Côte Sainte-Catherine, Montreal, Quebec H3T 1C4, Canada; University of Montreal, 2900 Boulevard Edouard-Monpetit, Montreal, Quebec H3T 1J4, Canada.
Caroline
C
Chartrand
Centre Hospitalier Universitaire Sainte-Justine, 3175 Chemin de la Côte Sainte-Catherine, Montreal, Quebec H3T 1C4, Canada; University of Montreal, 2900 Boulevard Edouard-Monpetit, Montreal, Quebec H3T 1J4, Canada. Electronic address: renaudchristian@hotmail.com.
Christian
C
Renaud
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000914:Antibodies, Viral / Q000097:blood*; D036022:Capsid Proteins / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D020031:Epstein-Barr Virus Infections / Q000175:diagnosis / Q000276:immunology / Q000821:virology; D019309:Epstein-Barr Virus Nuclear Antigens / Q000276:immunology*; D005260:Female; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D007118:Immunoassay / Q000379:methods / Q000592:standards; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D007223:Infant; D007231:Infant, Newborn; D008163:Luminescent Measurements; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D055815:Young Adult
D000914:Antibodies, Viral; D036022:Capsid Proteins; D019309:Epstein-Barr Virus Nuclear Antigens; D007074:Immunoglobulin G; D007075:Immunoglobulin M
Chemiluminescent microparticle immunoassay; Enzyme linked immunosorbent assay; Epstein-Barr virus; Immunofluorescence; Infectious mononucleosis