Bioconjugation Methods for Coupling Targeting Ligands with Fluorescent Dyes.
Targeted molecular imaging probes are essential tools for visualization of specific molecular processes in cells and living systems. Among these, targeted fluorescent probes are widely used due to the high sensitivity and resolution of fluorescence imaging. The conventional strategy for developing targeted fluorescent probes is to couple targeting ligands with fluorescent dyes by covalent bond via bioconjugation. Here, we describe several commonly used bioconjugation methods, from traditional amide and thiol coupling, to metal-catalyzed coupling reaction and catalyst free cycloaddition.
Department of Radiology, University of Pittsburgh, Suite 452G, 100 Technology Drive, Pittsburgh, PA, 15219, USA. lingx@upmc.edu.
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D057930:Click Chemistry; D061565:Cycloaddition Reaction / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D008024:Ligands; D057054:Molecular Imaging
D005456:Fluorescent Dyes; D008024:Ligands
Amide coupling; Bioconjugation; Click chemistry; Coupling reaction; Fluorescent probe; Huisgen cycloaddition; Inverse electron demand Diels–Alder cycloaddition; Michael addition; Molecular imaging; Suzuki coupling; Targeted; Thiol coupling