Synchrotron X-ray microtransections: a non invasive approach for epileptic seizures arising from eloquent cortical areas.
Synchrotron-generated X-ray (SRX) microbeams deposit high radiation doses to submillimetric targets whilst minimizing irradiation of neighboring healthy tissue. We developed a new radiosurgical method which demonstrably transects cortical brain tissue without affecting adjacent regions. We made such image-guided SRX microtransections in the left somatosensory cortex in a rat model of generalized epilepsy using high radiation doses (820 Gy) in thin (200 μm) parallel slices of tissue. This procedure, targeting the brain volume from which seizures arose, altered the abnormal neuronal activities for at least 9 weeks, as evidenced by a decrease of seizure power and coherence between tissue slices in comparison to the contralateral cortex. The brain tissue located between transections stayed histologically normal, while the irradiated micro-slices remained devoid of myelin and neurons two months after irradiation. This pre-clinical proof of concept highlights the translational potential of non-invasive SRX transections for treating epilepsies that are not eligible for resective surgery.
Inserm, U1216, F-38000 Grenoble, France.|Univ. Grenoble Alpes, Grenoble Institut des Neurosciences, GIN, F-38000 Grenoble, France.|Synapcell S.A.S - Bâtiment Biopolis - 5 avenue du Grand Sablon, La Tronche, France.
B
B
Pouyatos
ESRF, Grenoble, France.
C
C
Nemoz
Univ. Grenoble Alpes, EA RSRM, F-38000 Grenoble, France.
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T
Chabrol
Univ. Grenoble Alpes, Grenoble Institut des Neurosciences, GIN, F-38000 Grenoble, France.
M
M
Potez
ESRF, Grenoble, France.
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E
Bräuer
CNRS; CE2F PRIM UMS3537; Marseille, France.|Aix Marseille Université; Centre d'Exploration Fonctionnelle et de Formation; France.
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L
Renaud
Inserm, U1216, F-38000 Grenoble, France.|Univ. Grenoble Alpes, Grenoble Institut des Neurosciences, GIN, F-38000 Grenoble, France.
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K
Pernet-Gallay
Univ. Grenoble Alpes, EA RSRM, F-38000 Grenoble, France.
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F
Estève
Inserm, U1216, F-38000 Grenoble, France.|Univ. Grenoble Alpes, Grenoble Institut des Neurosciences, GIN, F-38000 Grenoble, France.
O
O
David
Inserm, U1216, F-38000 Grenoble, France.|Univ. Grenoble Alpes, Grenoble Institut des Neurosciences, GIN, F-38000 Grenoble, France.|CHU Grenoble Alpes, F-38000 Grenoble, France.
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P
Kahane
University of Bern, Switzerland.
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JA
Laissue
Inserm, U1216, F-38000 Grenoble, France.|Univ. Grenoble Alpes, Grenoble Institut des Neurosciences, GIN, F-38000 Grenoble, France.
A
A
Depaulis
Univ. Grenoble Alpes, EA RSRM, F-38000 Grenoble, France.
R
R
Serduc
D000818:Animals; D004195:Disease Models, Animal; D006801:Humans; D016634:Radiosurgery / Q000295:instrumentation*; D051381:Rats; D012640:Seizures / Q000503:physiopathology / Q000532:radiotherapy*; D013003:Somatosensory Cortex / Q000503:physiopathology / Q000528:radiation effects*; D017356:Synchrotrons