[Effect of Progesterone-induced MicroRNA-152 on the Proliferation of Endometrial Epithelial Cells].
OBJECTIVE
To determine the expression of microRNA-152 induced by progesterone and its effect on the proliferation of endometrial epithelial cells (EECs).


METHODS
Cultured EECs, Ishikawa were divided into four groups: control group (C group), 10(-8) mol/L estrogen treated group (E group), 10(-6) mol/L progesterone group (P group) and estrogen plus progesterone treated group (E&P group). The expression of mature microRNA-152 (microRNA-152-3p) of was detected by qRT-PCR. The estrogen treated cells were transfected with mimic-microRNA-152-3p. The estrogen and progesterone treated cells were transfected with inhibitor-microRNA-152-3p. Cell proliferations were detected by CCK-8 assay. The target gene of microRNA-152-3p proteins was predicted using microRNA target databases and validated by Western blot.


RESULTS
qRT-PCR showed no difference between C and E groups (P > 0.05) in the expression of microRNA-152-3p. P group had higher expressions of microRNA-152-3p than C group (P < 0.05). E&P group had higher expressions of microRNA-152-3p than C group and P group. MicroRNA target protein prediction suggested that CDC14A is one of direct target proteins of microRNA-152-3p. The results of CCK-8 assay showed that mimic-microRNA-152-3p transfection blocked proliferations of estrogen treated cells and lowered expressions of CDC14A in these cells; while inhibitor-microRNA-152-3p promotes proliferations of estrogen and progesterone treated cells and increased expressions of CDC14A in these cells.


CONCLUSIONS
Progesterone may suppress proliferations of EECs through inducing expressions of microRNA-152-3p. CDC14A is probably one target protein of microRNA-152-3p for its action on EECs.
Zhao You-bo
Dong-zhi
DZ
Yuan
Jun-li
JL
Pan
Li
L
Nie
Tin-hu
TH
Zhang
Ya-ping
YP
He
Li-min
LM
Yue
D015153:Blotting, Western; D049109:Cell Proliferation; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology*; D004967:Estrogens / Q000494:pharmacology; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology*; D014162:Transfection
D004967:Estrogens; C550856:MIRN152 microRNA, human; D035683:MicroRNAs; D011374:Progesterone