Recyclable magnetic nanocluster crosslinked with poly(ethylene oxide)-block-poly(2-vinyl-4,4-dimethylazlactone) copolymer for adsorption with antibody.
Surface modification of magnetic nanoparticle (MNP) with poly(ethylene oxide)-block-poly(2-vinyl-4,4-dimethylazlactone) (PEO-b-PVDM) diblock copolymers and its application as recyclable magnetic nano-support for adsorption with antibody were reported herein. PEO-b-PVDM copolymers were first synthesized via a reversible addition-fragmentation chain-transfer (RAFT) polymerization using poly(ethylene oxide) chain-transfer agent as a macromolecular chain transfer agent to mediate the RAFT polymerization of VDM. They were then grafted on amino-functionalized MNP by coupling with some azlactone rings of the PVDM block to form magnetic nanoclusters with tunable cluster size. The nanocluster size could be tuned by adjusting the chain length of the PVDM block. The nanoclusters were successfully used as efficient and recyclable nano-supports for adsorption with anti-rabbit IgG antibody. They retained higher than 95% adsorption of the antibody during eight adsorption-separation-desorption cycles, indicating the potential feasibility in using this novel hybrid nanocluster as recyclable support in cell separation applications.
Department of Chemistry and Center of Excellence in Biomaterials, Faculty of Science, Naresuan University, Phitsanulok 65000, Thailand.
Yingrak
Y
Prai-In
Department of Chemistry and Center of Excellence in Biomaterials, Faculty of Science, Naresuan University, Phitsanulok 65000, Thailand.
Chatchai
C
Boonthip
Department of Chemistry and Center of Excellence in Biomaterials, Faculty of Science, Naresuan University, Phitsanulok 65000, Thailand.
Boonjira
B
Rutnakornpituk
Department of Chemistry and Center of Excellence in Biomaterials, Faculty of Science, Naresuan University, Phitsanulok 65000, Thailand.
Uthai
U
Wichai
Institut des Molécules et des Matériaux du Mans, UMR CNRS 6283, Equipe Méthodologie et Synthèse des Polymères, Université du Maine, Avenue Olivier Messiaen, 72085 Le Mans Cedex, France.
Véronique
V
Montembault
Institut des Molécules et des Matériaux du Mans, UMR CNRS 6283, Equipe Méthodologie et Synthèse des Polymères, Université du Maine, Avenue Olivier Messiaen, 72085 Le Mans Cedex, France.
Sagrario
S
Pascual
Institut des Molécules et des Matériaux du Mans, UMR CNRS 6283, Equipe Méthodologie et Synthèse des Polymères, Université du Maine, Avenue Olivier Messiaen, 72085 Le Mans Cedex, France. Electronic address: laurent.fontaine@univ-lemans.fr.
Laurent
L
Fontaine
Department of Chemistry and Center of Excellence in Biomaterials, Faculty of Science, Naresuan University, Phitsanulok 65000, Thailand. Electronic address: methar@nu.ac.th.
Metha
M
Rutnakornpituk
D000327:Adsorption; D000906:Antibodies / Q000737:chemistry*; D000941:Antigens; D002850:Chromatography, Gel; D003432:Cross-Linking Reagents / Q000737:chemistry*; D057446:Hydrodynamics; D006863:Hydrogen-Ion Concentration; D007783:Lactones / Q000138:chemical synthesis / Q000737:chemistry*; D008280:Magnetics; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry*; D058105:Polymerization; D011145:Polyvinyls / Q000138:chemical synthesis / Q000737:chemistry*; D066244:Proton Magnetic Resonance Spectroscopy; D059027:Recycling; D012996:Solutions; D017550:Spectroscopy, Fourier Transform Infrared; D055672:Static Electricity; D013818:Thermogravimetry
D000906:Antibodies; D000941:Antigens; D003432:Cross-Linking Reagents; D007783:Lactones; D011145:Polyvinyls; D012996:Solutions; C538770:poly(vinyldimethylazlactone); D011092:Polyethylene Glycols
Adsorption; Antibody; Azlactone; Magnetite; Nanocluster; RAFT