[Fabrication of bioactive tissue engineering scaffold for reconstructing calcified cartilage layer based on three-dimension printing technique].
To fabricate organic-inorganic composite tissue engineering scaffolds for reconstructing calcified cartilage layer based on three-dimensional (3D) printing technique.



The scaffolds were developed by 3D-printing technique with highly bioactive calcium-magnesium silicate ultrafine particles of 1%, 3% and 5% of mass fraction, in which the organic phases were composed of type I collagen and sodium hyaluronate. The 3D-printed scaffolds were then crosslinked and solidified by alginate and CaCl₂ aerosol. The pore size and distribution of inorganic phase were observed with scanning electron microscope (SEM); the mechanical properties were tested with universal material testing machine, and the porosity of scaffolds was also measured.



Pore size was approximately (212.3 ± 34.2) μm with a porosity of (48.3 ± 5.9)%, the compressive modulus of the scaffolds was (7.2 ± 1.2) MPa, which was irrelevant to the percentage changes of calcium-magnesium silicate, the compressive modulus was between that of cartilage and subchondral bone.



The porous scaffolds for calcified cartilage layer have been successfully fabricated, which would be used for multi-layered composite scaffolds in osteochondral injury.
Department of Orthopedic Surgery, Orthopedics Research Institute of Zhejiang University, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China.
Xinning
X
Yu
Department of Orthopedic Surgery, Orthopedics Research Institute of Zhejiang University, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China.
Jinghua
J
Fang
Department of Orthopedic Surgery, Orthopedics Research Institute of Zhejiang University, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China.
Jianyang
J
Luo
Zhejiang-California International Nanosystems Institute, Hangzhou 310058, China.
Xianyan
X
Yang
Zhejiang-California International Nanosystems Institute, Hangzhou 310058, China.
Dongshuang
D
He
Zhejiang-California International Nanosystems Institute, Hangzhou 310058, China. zhrgou@zju.edu.cn.
Zhongru
Z
Gou
Department of Orthopedic Surgery, Orthopedics Research Institute of Zhejiang University, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China. daixshz@163.com.
Xuesong
X
Dai
D062028:Bioprinting; D002356:Cartilage / Q000254:growth & development*; D008422:Materials Testing; D016062:Porosity; D066330:Printing, Three-Dimensional; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*