Complete genome sequence of Streptococcus agalactiae strain GBS85147 serotype of type Ia isolated from human oropharynx.
Streptococcus agalactiae, also referred to as Group B Streptococcus, is a frequent resident of the rectovaginal tract in humans, and a major cause of neonatal infection. The pathogen can also infect adults with underlying disease, particularly the elderly and immunocompromised ones. In addition, S. agalactiae is a known fish pathogen, which compromises food safety and represents a zoonotic hazard. This study provides valuable structural, functional and evolutionary genomic information of a human S. agalactiae serotype Ia (ST-103) GBS85147 strain isolated from the oropharynx of an adult patient from Rio de Janeiro, thereby representing the first human isolate in Brazil. We used the Ion Torrent PGM platform with the 200 bp fragment library sequencing kit. The sequencing generated 578,082,183 bp, distributed among 2,973,022 reads, resulting in an approximately 246-fold mean coverage depth and was assembled using the Mira Assembler v3.9.18. The S. agalactiae strain GBS85147 comprises of a circular chromosome with a final genome length of 1,996,151 bp containing 1,915 protein-coding genes, 18 rRNA, 63 tRNA, 2 pseudogenes and a G + C content of 35.48 %.
Laboratory of Cellular and Molecular Genetics (LGCM), Federal University of Minas Gerais, Belo Horizonte, Brazil.
Edgar Lacerda
EL
de Aguiar
Laboratory of Cellular and Molecular Genetics (LGCM), Federal University of Minas Gerais, Belo Horizonte, Brazil.
Diego César Batista
DC
Mariano
Laboratory of Cellular and Molecular Genetics (LGCM), Federal University of Minas Gerais, Belo Horizonte, Brazil.
Marcus Vinícius Canário
MV
Viana
Laboratory of Cellular and Molecular Genetics (LGCM), Federal University of Minas Gerais, Belo Horizonte, Brazil.
Leandro de Jesus
Lde J
Benevides
Laboratory of Cellular and Molecular Genetics (LGCM), Federal University of Minas Gerais, Belo Horizonte, Brazil.
Flávia
F
de Souza Rocha
Laboratory of Cellular and Molecular Genetics (LGCM), Federal University of Minas Gerais, Belo Horizonte, Brazil.
Letícia
L
de Castro Oliveira
National Reference Laboratory for Aquatic Animal Diseases (AQUACEN), Ministry of Fisheries and Aquaculture, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Felipe Luiz
FL
Pereira
National Reference Laboratory for Aquatic Animal Diseases (AQUACEN), Ministry of Fisheries and Aquaculture, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Fernanda Alves
FA
Dorella
National Reference Laboratory for Aquatic Animal Diseases (AQUACEN), Ministry of Fisheries and Aquaculture, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Carlos Augusto Gomes
CA
Leal
National Reference Laboratory for Aquatic Animal Diseases (AQUACEN), Ministry of Fisheries and Aquaculture, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Alex Fiorini
AF
de Carvalho
Laboratory of Molecular Biology and Physiology of Streptococci, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
Gabriela Silva
GS
Santos
Faculty of Medical Sciences - State University of Rio de Janeiro, Rio de Janeiro, Brazil.
Ana Luiza
AL
Mattos-Guaraldi
Laboratory of Molecular Biology and Physiology of Streptococci, State University of Rio de Janeiro, Rio de Janeiro, Brazil.
Prescilla Emy
PE
Nagao
Institute of Biological and Natural Sciences (ICBN)- Federal University of Triangulo Mineiro, Uberaba, Minas Gerais Brazil.
Siomar
S
de Castro Soares
Laboratory of Cellular and Molecular Genetics (LGCM), Federal University of Minas Gerais, Belo Horizonte, Brazil.
Syed Shah
SS
Hassan
Laboratory of Cellular and Molecular Genetics (LGCM), Federal University of Minas Gerais, Belo Horizonte, Brazil.
Anne Cybele
AC
Pinto
National Reference Laboratory for Aquatic Animal Diseases (AQUACEN), Ministry of Fisheries and Aquaculture, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Henrique César Pereira
HC
Figueiredo
Laboratory of Cellular and Molecular Genetics (LGCM), Federal University of Minas Gerais, Belo Horizonte, Brazil.
Vasco
V
Azevedo
Complete genome sequence; Human pathogenic bacteria; Ion torrent; Oropharynx; Streptococcus agalactiae