Second bioluminescence-activating component in the luminous fungus Mycena chlorophos.
Mycena chlorophos is an oxygen-dependent bioluminescent fungus. The mechanisms underlying its light emission are unknown. A component that increased the bioluminescence intensity of the immature living gills of M. chlorophos was isolated from mature M. chlorophos gills and chemically characterized. The bioluminescence-activating component was found to be trans-3,4-dihydroxycinnamic acid and its bioluminescence activation was highly structure-specific.13C- and18O-labelling studies using the immature living gills showed that trans-3,4-dihydroxycinnamic acid was synthesized from trans-4-hydroxycinnamic acid in the gills by hydroxylation with molecular oxygen as well as by the general metabolism, and trans-3,4-dihydroxycinnamic acid did not produce hispidin (detection-limit concentration: 10 pmol/1 g wet gill). Addition of 0.01 mM hispidin to the immature living gills generated no bioluminescence activation. These results suggested that the prompt bioluminescence activation resulting from addition of trans-3,4-dihydroxycinnamic acid could not be attributed to the generation of hispidin. Copyright © 2016 John Wiley & Sons, Ltd.
Graduate School of Bioresources, Mie University, 1577 Kurimamachiya, Tsu, Mie, Japan.
Katsunori
K
Teranishi
D001487:Basidiomycota / Q000737:chemistry*; D048690:Fruiting Bodies, Fungal / Q000737:chemistry*; D049449:Luminescence; D008163:Luminescent Measurements
Mycena chlorophos; bioluminescence; fungus; metabolism; trans-3,4-dihydroxycinnamic acid