Antibacterial activities of fluorescent nano assembled triphenylamine phosphonium ionic liquids.
Staphylococcus aureus, a Gram positive coccal bacterium is a major cause of nosocomial infection. We report the synthesis of new triphenylamine phosphonium ionic liquids which are able to self-assemble into multiwall nanoassemblies and to reveal a strong bactericidal activity (MIC=0.5mg/L) for Gram positive bacteria (including resistant strains) comparable to that of standard antibiotics. Time kill, metabolism and fluorescence confocal microscopy studies show a quasi-instantaneously penetration of the nanoassemblies inside the bacteria resulting of a rapid blocking (30min) of their proliferation. As confirmed by rezasurin reduction monitoring, these compounds strongly affect the bacterial metabolism and a Gram positive versus Gram negative selectivity is clearly observed. These fluorescent phosphonium ionic liquid might constitute a useful tool for both translocation studies and to tackle infectious diseases related to the field of implantology.
Aix-Marseille Université, CNRS, Centre Interdisciplinaire de Nanoscience de Marseille, UMR 7325, UMR 7325163, avenue de Luminy, 13288 Marseille, France.
Frédéric
F
Brunel
Aix-Marseille Université, IRBA, Transporteurs Membranaires, Chimiorésistance et Drug-Design (TMCD2), UMR-MD1, 27 boulevard Jean Moulin, 13385 Marseille cedex 05, France.
Christelle
C
Lautard
Aix-Marseille Université, CNRS, Centre Interdisciplinaire de Nanoscience de Marseille, UMR 7325, UMR 7325163, avenue de Luminy, 13288 Marseille, France.
Frédéric
F
Garzino
Aix-Marseille Université, CNRS, Centre Interdisciplinaire de Nanoscience de Marseille, UMR 7325, UMR 7325163, avenue de Luminy, 13288 Marseille, France.
Suzanne
S
Giorgio
Aix-Marseille Université, CNRS, Centre Interdisciplinaire de Nanoscience de Marseille, UMR 7325, UMR 7325163, avenue de Luminy, 13288 Marseille, France. Electronic address: jean-manuel.raimundo@univ-amu.fr.
Jean M
JM
Raimundo
Aix-Marseille Université, IRBA, Transporteurs Membranaires, Chimiorésistance et Drug-Design (TMCD2), UMR-MD1, 27 boulevard Jean Moulin, 13385 Marseille cedex 05, France. Electronic address: jean-michel.bolla@univ-amu.fr.
Jean M
JM
Bolla
Aix-Marseille Université, CNRS, Centre Interdisciplinaire de Nanoscience de Marseille, UMR 7325, UMR 7325163, avenue de Luminy, 13288 Marseille, France. Electronic address: michel.camplo@univ-amu.fr.
Michel
M
Camplo
D000588:Amines / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D001419:Bacteria / Q000166:cytology / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D052578:Ionic Liquids / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D009943:Organophosphorus Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship
D000588:Amines; D000900:Anti-Bacterial Agents; D005456:Fluorescent Dyes; D052578:Ionic Liquids; D009943:Organophosphorus Compounds
Antimicrobial; Fluorescent probes; Phosphonium ionic liquids; Staphylococcus aureus; Triphenylamine