Characteristic chromatographic fingerprint study of short-chain fatty acids in human milk, infant formula, pure milk and fermented milk by gas chromatography-mass spectrometry.
Human milk, infant formula, pure milk and fermented milk as food products or dietary supplements provide a range of nutrients required to both infants and adults. Recently, a growing body of evidence has revealed the beneficial roles of short-chain fatty acids (SCFAs), a subset of fatty acids produced from the fermentation of dietary fibers by gut microbiota. The objective of this study was to establish a chromatographic fingerprint technique to investigate SCFAs in human milk and dairy products by gas chromatography coupled with mass spectrometry. The multivariate method for principal component analysis assessed differences between milk types. Human milk, infant formula, pure milk and fermented milk were grouped independently, mainly because of differences in formic acid, acetic acid, propionic acid and hexanoic acid levels. This method will be important for the assessment of SCFAs in human milk and various dairy products.
a Tianjin State Key Laboratory of Modern Chinese Medicine , Tianjin University of Traditional Chinese Medicine , Tianjin , PR China ;|c Research and Development Center of TCM , Tianjin International Joint Academy of Biotechnology and Medicine , Tianjin , PR China.
Zhenzuo
Z
Jiang
b College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine , Tianjin , PR China ;
Yanan
Y
Liu
a Tianjin State Key Laboratory of Modern Chinese Medicine , Tianjin University of Traditional Chinese Medicine , Tianjin , PR China ;|c Research and Development Center of TCM , Tianjin International Joint Academy of Biotechnology and Medicine , Tianjin , PR China.
Yan
Y
Zhu
a Tianjin State Key Laboratory of Modern Chinese Medicine , Tianjin University of Traditional Chinese Medicine , Tianjin , PR China ;|c Research and Development Center of TCM , Tianjin International Joint Academy of Biotechnology and Medicine , Tianjin , PR China.
Jing
J
Yang
b College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine , Tianjin , PR China ;
Lili
L
Sun
a Tianjin State Key Laboratory of Modern Chinese Medicine , Tianjin University of Traditional Chinese Medicine , Tianjin , PR China ;|c Research and Development Center of TCM , Tianjin International Joint Academy of Biotechnology and Medicine , Tianjin , PR China.
Xin
X
Chai
a Tianjin State Key Laboratory of Modern Chinese Medicine , Tianjin University of Traditional Chinese Medicine , Tianjin , PR China ;|c Research and Development Center of TCM , Tianjin International Joint Academy of Biotechnology and Medicine , Tianjin , PR China.
Yuefei
Y
Wang
D000818:Animals; D002417:Cattle; D043302:Cultured Milk Products / Q000032:analysis*; D003611:Dairy Products; D005232:Fatty Acids, Volatile / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D041943:Infant Formula / Q000737:chemistry*; D008895:Milk, Human / Q000737:chemistry*
D005232:Fatty Acids, Volatile
Characteristic chromatographic fingerprint; gas chromatography–mass spectrometry; human milk and dairy products; principal component analysis; short-chain fatty acids