Intervention of pumpkin seed oil on metabolic disease revealed by metabonomics and transcript profile.
BACKGROUND
Understanding the metabolic and transcription basis of pumpkin seed oil (PSO) intervention on metabolic disease (MD) is essential to daily nutrition and health.


RESULTS
This study analyzed the liver metabolic variations of Wistar rats fed normal diet (CON), high-fat diet (HFD) and high-fat plus PSO diet (PSO) to establish the relationship between the liver metabolite composition/transcript profile and the effects of PSO on MD. By using proton nuclear magnetic resonance spectroscopy together with multivariate data analysis, it was found that, compared with CON rats, HFD rats showed clear dysfunctions of choline metabolism, glucose metabolism and nucleotide and amino acid metabolism. Using quantitative real-time polymerase chain reaction (qPCR), it was found that, compared with HFD rats, PSO rats showed alleviated endoplasmic reticulum stress accompanied by lowered unfolded protein response.


CONCLUSIONS
These findings provide useful information to understand the metabolic alterations triggered by MD and to evaluate the effects of PSO intervention. © 2016 Society of Chemical Industry.
School of Biology and Pharmaceutical Engineering, Collaborative Innovation Center for Processing of Agricultural Products (Hubei Province), Wuhan Polytechnic University, No. 68 South Xuefu Road, Changqing Garden, Wuhan, 430023, China.|Hubei Key Laboratory of Lipid Chemistry and Nutrition of Oil, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, 430062, China.
Xiu-Ju
XJ
Zhao
School of Biology and Pharmaceutical Engineering, Collaborative Innovation Center for Processing of Agricultural Products (Hubei Province), Wuhan Polytechnic University, No. 68 South Xuefu Road, Changqing Garden, Wuhan, 430023, China.
Yu-Lian
YL
Chen
Changyuan Cuisine Vocational and Technical College, Changyuan, 453400, China.
Bing
B
Fu
School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi'an, 710021, China.
Wen
W
Zhang
School of Biology and Pharmaceutical Engineering, Collaborative Innovation Center for Processing of Agricultural Products (Hubei Province), Wuhan Polytechnic University, No. 68 South Xuefu Road, Changqing Garden, Wuhan, 430023, China.
Zhiguo
Z
Liu
Xinxiang Institute for Drug Control, Food and Drug Administration of Xinxiang, No. 17 Jiankang Road, Xinxiang, 453000, China.
Hexian
H
Zhuo
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002794:Choline / Q000378:metabolism; D028464:Cucurbita / Q000737:chemistry*; D059305:Diet, High-Fat / Q000009:adverse effects*; D004041:Dietary Fats; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects*; D005947:Glucose / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008659:Metabolic Diseases / Q000188:drug therapy / Q000209:etiology / Q000378:metabolism*; D055442:Metabolome; D055432:Metabolomics; D009711:Nucleotides / Q000378:metabolism; D010938:Plant Oils / Q000494:pharmacology*; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D012639:Seeds / Q000737:chemistry; D014158:Transcription, Genetic; D056811:Unfolded Protein Response / Q000187:drug effects
D000596:Amino Acids; D004041:Dietary Fats; D009711:Nucleotides; D010938:Plant Oils; D005947:Glucose; D002794:Choline
metabolic disease (MD); metabonomics; pumpkin seed oil (PSO); transcript profile