Validity of leptin receptor-deficiency (db/db) type 2 diabetes mellitus mice as a model of secondary osteoporosis.
This study aimed to evaluate the validation of the leptin receptor-deficient mice model for secondary osteoporosis associated with type 2 diabetes mellitus (T2DM) at bone micro-architectural level. Thirty three 36-week old male mice were divided into four groups: normal control (db/m) (n = 7), leptin receptor-deficient T2DM (db/db) (n = 8), human C-reactive protein (CRP) transgenic normal control (crp/db/m) (n = 7), and human CRP transgenic T2DM (crp/db/db) (n = 11). Lumber vertebrae (L5) and bilateral lower limbs were scanned by micro-CT to analyze trabecular and cortical bone quality. Right femora were used for three-point bending to analyze the mechanical properties. Trabecular bone quality at L5 was better in db/db or crp/db/db group in terms of bone mineral density (BMD), bone volume fraction, connectivity density, trabecular number and separation (all p < 0.05). However the indices measured at proximal tibia showed comparable trabecular BMD and microarchitecture among the four groups. Femur length in crp/db/db group was significantly shorter than db/m group (p < 0.05) and cortices were thinner in db/db and crp/db/db groups (p > 0.05). Maximum loading and energy yield in mechanical test were similar among groups while the elastic modulus in db/db and crp/db/db significantly lower than db/m. The leptin-receptor mice is not a proper model for secondary osteoporosis associated with T2DM.
Musculoskeletal Research Laboratory, Department of Orthopaedics and Traumatology, the Chinese University of Hong Kong, Hong Kong SAR, China.
Le
L
Huang
Department of Medicine &Therapeutics, the Chinese University of Hong Kong, Hong Kong SAR, China.
Yong-Ke
YK
You
Bone Quality and Health Assessment Centre, Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Hong Kong SAR, China.
Tracy Y
TY
Zhu
Musculoskeletal Research Laboratory, Department of Orthopaedics and Traumatology, the Chinese University of Hong Kong, Hong Kong SAR, China.
Li-Zhen
LZ
Zheng
Department of Medicine &Therapeutics, the Chinese University of Hong Kong, Hong Kong SAR, China.
Xiao-Ru
XR
Huang
Department of Medicine &Therapeutics, the Chinese University of Hong Kong, Hong Kong SAR, China.
Hai-Yong
HY
Chen
Musculoskeletal Research Laboratory, Department of Orthopaedics and Traumatology, the Chinese University of Hong Kong, Hong Kong SAR, China.
Dong
D
Yao
Department of Medicine &Therapeutics, the Chinese University of Hong Kong, Hong Kong SAR, China.
Hui-Yao
HY
Lan
Musculoskeletal Research Laboratory, Department of Orthopaedics and Traumatology, the Chinese University of Hong Kong, Hong Kong SAR, China.|Bone Quality and Health Assessment Centre, Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Hong Kong SAR, China.
Ling
L
Qin
D000818:Animals; D001696:Biomechanical Phenomena; D001835:Body Weight; D015519:Bone Density; D001842:Bone and Bones / Q000000981:diagnostic imaging / Q000473:pathology / Q000503:physiopathology; D000071556:Cancellous Bone / Q000473:pathology; D003924:Diabetes Mellitus, Type 2 / Q000150:complications* / Q000473:pathology* / Q000503:physiopathology; D004195:Disease Models, Animal; D021621:Imaging, Three-Dimensional; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D010024:Osteoporosis / Q000150:complications* / Q000000981:diagnostic imaging / Q000473:pathology* / Q000503:physiopathology; D054411:Receptors, Leptin / Q000172:deficiency* / Q000378:metabolism; D015203:Reproducibility of Results; D055114:X-Ray Microtomography
D054411:Receptors, Leptin; C515447:leptin receptor, mouse