Embryonic cell culture in zebrafish.
Zebrafish embryonic cell cultures have many useful properties that make them complementary to intact embryos for a wide range of studies. Embryonic cell cultures allow for maintenance of transient cell populations, control of chemical and mechanical cues received by cells, and facile chemical screening. Zebrafish cells can be cultured in either heterogeneous or homogeneous cultures from a wide range of developmental time points. Here we describe two methods with particular applicability to chemical screening: a method for the culture of blastomeres for directed differentiation toward the myogenic lineage and a method for the culture of neural crest cells in heterogeneous cultures from early somitogenesis embryos.
Harvard Medical School and Children's Hospital, Boston, MA, United States.
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Ciarlo
Children's Hospital and Dana Farber Cancer Institute, Boston, MA, United States; Harvard University, Cambridge, MA, United States.
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D000818:Animals; D001757:Blastomeres / Q000166:cytology*; D018929:Cell Culture Techniques; D004625:Embryo, Nonmammalian / Q000166:cytology*; D047108:Embryonic Development; D009432:Neural Crest / Q000166:cytology; D015027:Zebrafish / Q000254:growth & development*
Blastomere; Cell culture; Chemical screening; Embryonic stem cells; Myogenic progenitor; Neural crest