Sorokiniol: a new enzymes inhibitory metabolite from fungal endophyte Bipolaris sorokiniana LK12.
BACKGROUND
Medicinal plants harboring endophytic fungi could carry significant potential for producing bioactive secondary metabolites. Endophytic fungi serve as alternate source of interesting compounds in their natural and modified synthetic forms to treat different diseases. In this regard, endophytic microflora associated with alkaloid-rich medicinal plants Rhazya stricta is least known.


RESULTS
We isolated one new bioactive compound sorokiniol (1) along with two known cyclic peptides BZR-cotoxin I (2) and BZR-cotoxin IV (3) from fungal endophyte Bipolaris sorokiniana LK12. The structures of the isolated new and known compounds were elucidated through spectroscopic data, including 1D and 2D NMR ((1)H, (13)C, HSQC, HMBC, and NOESY), mass, and UV. The known peptides (2-3) were characterized by ESI-MS, MS/MS, and by comparing the NMR data with the literature. The isolated metabolites were assayed for their role against enzyme inhibition. Compound 1 was significantly inhibitory towards acetyl cholinestrase while the other compounds (2-3) had moderate anti-lipid peroxidation and urease activities.


CONCLUSIONS
The present results suggest that the endophytic microorganism associated with indigenously important medicinal plants can offer a rich source of biologically active chemical constituents which could help in discovering enzyme inhibitory lead drugs.
UoN Chair of Oman's Medicinal Plants and Marine Natural Products, University of Nizwa, Nizwa, 616, Sultanate of Oman.
Liaqat
L
Ali
UoN Chair of Oman's Medicinal Plants and Marine Natural Products, University of Nizwa, Nizwa, 616, Sultanate of Oman. latifepm78@yahoo.co.uk.
Abdul Latif
AL
Khan
Department of Biological Sciences and Chemistry, College of Arts and Sciences, University of Nizwa, Birkat Al-Mouz, Nizwa, 616, Sultanate of Oman.
Javid
J
Hussain
UoN Chair of Oman's Medicinal Plants and Marine Natural Products, University of Nizwa, Nizwa, 616, Sultanate of Oman. aharrasi@unizwa.edu.om.
Ahmed
A
Al-Harrasi
School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.|Department of Agriculture, Abdul Wali Khan University, Mardan, Pakistan.
Muhammad
M
Waqas
School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
Sang-Mo
SM
Kang
UoN Chair of Oman's Medicinal Plants and Marine Natural Products, University of Nizwa, Nizwa, 616, Sultanate of Oman.
Ahmed
A
Al-Rawahi
School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea. ijlee@knu.ac.kr.
In-Jung
IJ
Lee
D029062:Apocynaceae / Q000382:microbiology*; D001203:Ascomycota / Q000737:chemistry / Q000254:growth & development*; D002800:Cholinesterase Inhibitors / Q000302:isolation & purification / Q000494:pharmacology; D060026:Endophytes / Q000737:chemistry / Q000254:growth & development; D004791:Enzyme Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D064210:Secondary Metabolism; D053719:Tandem Mass Spectrometry
D002800:Cholinesterase Inhibitors; D004791:Enzyme Inhibitors
Bipolaris sorokiniana LK12; Cyclic peptides; Endophytic fungi; Enzyme inhibition and antioxidant; Secondary metabolites; Structure elucidation