Analysis of the miRNA-mRNA networks in malignant transformation BEAS-2B cells induced by alpha-particles.
The aim of this study was to determine the toxicity induced by irradiation with alpha-particles on malignant transformation of immortalized human bronchial epithelial cells (BEAS-2B) using miRNA-mRNA networks. The expression of BEAS-2B cells was determined by measuring colony formation, mtDNA, mitochondrial membrane potential (MMP), and ROS levels. Changes in BEAS-2B cell gene expression were observed and quantified using microarrays that included an increase in 157 mRNA and 20 miRNA expression and a decrease in 77 mRNA and 48 miRNA. Bioinformatic software was used to analyze these different mRNA and miRNA, which indicated that miR-107 and miR-494 play an important role in alpha-particles-mediated cellular malignant transformation processes. The pathways related to systemic lupus erythematosus, cytokine-cytokine receptor interaction, MAPK signaling pathway, regulation of actin cytoskeleton, and cell adhesion molecules (CAMs) were stimulated, while those of ribosome, transforming growth factor (TGF)-beta signaling pathway, and metabolic pathways were inhibited. Data suggest that miRNA and mRNA play a crucial role in alpha-particles-mediated malignant transformation processes. It is worth noting that three target genes associated with lung cancer were identified and upregulated PEG 10 (paternally expressed gene 10), ARHGAP26, and IRS1.
a School of Public Health , Medical College of Soochow University , Suzhou , China.|b Jiangsu Key Laboratory of Preventive and Translational Medicine for Genetic Diseases , Suzhou , China.|c School of Radiation Medicine and Protection and Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection , Medical College of Soochow University , Suzhou , China.
Ji-Hua
JH
Nie
a School of Public Health , Medical College of Soochow University , Suzhou , China.|b Jiangsu Key Laboratory of Preventive and Translational Medicine for Genetic Diseases , Suzhou , China.
Zhi-Hai
ZH
Chen
e Institute of Radiation Medicine , Fudan University , Shanghai , China.
Chun-Lin
CL
Shao
a School of Public Health , Medical College of Soochow University , Suzhou , China.|b Jiangsu Key Laboratory of Preventive and Translational Medicine for Genetic Diseases , Suzhou , China.
Wei-Wei
WW
Pei
a School of Public Health , Medical College of Soochow University , Suzhou , China.
Jie
J
Zhang
c School of Radiation Medicine and Protection and Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection , Medical College of Soochow University , Suzhou , China.|d Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions and School for Radiological and Interdisciplinary Sciences (RAD-X) , Soochow University , Suzhou , China.
Shu-Yu
SY
Zhang
c School of Radiation Medicine and Protection and Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection , Medical College of Soochow University , Suzhou , China.|d Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions and School for Radiological and Interdisciplinary Sciences (RAD-X) , Soochow University , Suzhou , China.
Yang
Y
Jiao
a School of Public Health , Medical College of Soochow University , Suzhou , China.|b Jiangsu Key Laboratory of Preventive and Translational Medicine for Genetic Diseases , Suzhou , China.
Jian
J
Tong
D000512:Alpha Particles / Q000009:adverse effects*; D001980:Bronchi / Q000528:radiation effects; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000378:metabolism / Q000528:radiation effects*; D004847:Epithelial Cells / Q000528:radiation effects*; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects; D006801:Humans; D008175:Lung Neoplasms / Q000209:etiology; D035683:MicroRNAs / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D015398:Signal Transduction / Q000528:radiation effects*
D035683:MicroRNAs; D012333:RNA, Messenger