Repeatability and reproducibility of optic nerve head perfusion measurements using optical coherence tomography angiography.
Optical coherence tomography angiography (OCTA) has increasingly become a clinically useful technique in ophthalmic imaging. We evaluate the repeatability and reproducibility of blood perfusion in the optic nerve head (ONH) measured using optical microangiography (OMAG)-based OCTA. Ten eyes from 10 healthy volunteers are recruited and scanned three times with a 68-kHz Cirrus HD-OCT 5000-based OMAG prototype system (Carl Zeiss Meditec Inc., Dublin, California) centered at the ONH involving two separate visits within six weeks. Vascular images are generated with OMAG processing by detecting the differences in OCT signals between consecutive B-scans acquired at the same retina location. ONH perfusion is quantified as flux, vessel area density, and normalized flux within the ONH for the prelaminar, lamina cribrosa, and the full ONH. Coefficient of variation (CV) and intraclass correlation coefficient (ICC) are used to evaluate intravisit and intervisit repeatability, and interobserver reproducibility. ONH perfusion measurements show high repeatability [CV≤3.7% (intravisit) and ≤5.2% (intervisit)] and interobserver reproducibility (ICC≤0.966) in all three layers by three metrics. OCTA provides a noninvasive method to visualize and quantify ONH perfusion in human eyes with excellent repeatability and reproducibility, which may add additional insight into ONH perfusion in clinical practice.
University of Washington, Department of Bioengineering, 3720 15th Avenue NE, Seattle, Washington 98195, United StatesbUniversity of Washington, Department of Ophthalmology, 908 Jefferson Street, Seattle, Washington 98104, United States.
Chieh-Li
CL
Chen
University of Washington, Department of Ophthalmology, 908 Jefferson Street, Seattle, Washington 98104, United States.
Karine D
KD
Bojikian
University of Washington, Department of Bioengineering, 3720 15th Avenue NE, Seattle, Washington 98195, United States.
Chen
C
Xin
University of Washington, Department of Ophthalmology, 908 Jefferson Street, Seattle, Washington 98104, United States.
Joanne C
JC
Wen
University of Washington, Department of Ophthalmology, 908 Jefferson Street, Seattle, Washington 98104, United States.
Divakar
D
Gupta
University of Washington, Department of Bioengineering, 3720 15th Avenue NE, Seattle, Washington 98195, United States.
Qinqin
Q
Zhang
University of Washington, Department of Ophthalmology, 908 Jefferson Street, Seattle, Washington 98104, United States.
Raghu C
RC
Mudumbai
University of Washington, Department of Ophthalmology, 908 Jefferson Street, Seattle, Washington 98104, United States.
Murray A
MA
Johnstone
University of Washington, Department of Ophthalmology, 908 Jefferson Street, Seattle, Washington 98104, United States.
Philip P
PP
Chen
University of Washington, Department of Bioengineering, 3720 15th Avenue NE, Seattle, Washington 98195, United StatesbUniversity of Washington, Department of Ophthalmology, 908 Jefferson Street, Seattle, Washington 98104, United States.
Ruikang K
RK
Wang
D000792:Angiography / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D009898:Optic Disk / Q000098:blood supply* / Q000000981:diagnostic imaging*; D015203:Reproducibility of Results; D041623:Tomography, Optical Coherence / Q000592:standards