[Expert consensus on induction of human embryonic stem cells into tenocytes].
Embryonic stem cells have unlimited proliferative capacity, which may provide a source of tendon stem/progenitor cells for tissue engineering. Experts of International Science and Technology Collaborative Program of Ministry of Science and Technology have developed a protocol consensus on differentiation of human embryonic stem cells into the tendon cells. The consensus recommends a protocol of two-step generation of human embryonic stem cells into tendon cells: the human embryonic stem cells are first differentiated into mesenchymal stem cells on different material surfaces; then with the scaffold-free tissue engineering tendon formed by high-density planting, the mesenchymal stem cells are induced into tendon cells under static or dynamic mechanical stimulation in vivo and in vitro. Tissue engineering tendon established in vitro by the protocol can be used as a model in toxicological analysis and safety evaluation of tendon-relevant small molecule compounds, medical materials and drugs.
Center of Stem Cell and Tissue Engineering, Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Key Laboratory of Stem Cell of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou 310058, China.
Xiao
X
Chen
Central Laboratory, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
Xiaohui
X
Zou
Department of Oral and Maxillofacial Surgery, Peking University School of Stomatology, Beijing 100081, China.
Guangyan
G
Yu
Research Center of Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Beijing 100144, China.
Xin
X
Fu
Faculty of Dentistry Research Laboratories, National University of Singapore, Singapore 119083, Singapore.
Tong
T
Cao
Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove QLD 4059, Australia.
Yin
Y
Xiao
Center of Stem Cell and Tissue Engineering, Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Key Laboratory of Stem Cell of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou 310058, China. hwoy@zju.edu.cn.
Hongwei
H
Ouyang
D002454:Cell Differentiation; D032921:Consensus; D000066449:Human Embryonic Stem Cells / Q000166:cytology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology; D013710:Tendons / Q000166:cytology*; D023822:Tissue Engineering