A simple LC-ESI-MS/MS method for quantification of bacopaside I in rat plasma and its application to a pharmacokinetic study.
Bacopaside I is one of the main pseudojujubogenin glycosides isolated from Bacopa monniera. In the present study, a rapid and robust LC-ESI-MS/MS method was developed and validated to quantify bacopaside I in rat plasma. After plasma samples were deproteinized by methanol, the post-treatment samples were analyzed on a Zorbax Eclipse Plus C18 (2.1×50mm, 1.8μm) column using a mobile phase of acetonitrile and water (65:35, v/v). Detection was performed on a triple-quadrupole tandem mass spectrometer with selected reaction monitoring (SRM) mode via electrospray ionization source. This method covered a linearity range of 10-2000ng/mL with the lower limit of quantification of 10ng/mL. The intra- and inter-day precisions of analysis were less than 10.2%, and the accuracies were between -11.1% and 8.4% at the concentrations of 25, 150 and 1800ng/mL. The total run time was 6.0min. This method was successfully applied to the preclinical pharmacokinetic study of bacopaside I following intravenous or oral administration to rats.
Department of Orthopedics, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, China.
Ruo-Feng
RF
Yin
Department of Anatomy and Neurobiology, School of Basic Medical Science Central South University, Changsha 410013, China.
Kun
K
Xiong
Department of Clinical Epidemiology, the First Hospital of Jilin University, Changchun 130021, China.
Si-Min
SM
Wen
Department of Anesthesia, the Second Hospital of Jilin University, No. 218 Ziqiang Street, Changchun 130021, China. Electronic address: songhkasfha@sina.com.
Zhi-Min
ZM
Song
Department of Spine Surgery, the First Hospital of Jilin University, No. 71 Xinmin Street, Changchun 130021, China. Electronic address: feng_xu_fsf@sina.com.
Feng
F
Xu
D000818:Animals; D034681:Bacopa / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057230:Limit of Detection; D008297:Male; D018696:Neuroprotective Agents / Q000032:analysis / Q000097:blood*; D017208:Rats, Wistar; D012503:Saponins / Q000032:analysis / Q000097:blood*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014315:Triterpenes / Q000032:analysis / Q000097:blood*
D018696:Neuroprotective Agents; D012503:Saponins; D014315:Triterpenes; C442170:bacopaside I
Bacopaside I; LC-ESI–MS/MS; Pharmacokinetics; Rat plasma