Polymorphism of IL-10 gene promoter region: association with T cell proliferative responses after acellular pertussis vaccination in adults.
Individual variation in immune responses is always encountered after vaccination. This phenomenon is also seen after acellular pertussis vaccination. The aim of this present study was to investigate whether single nucleotide polymorphisms (SNPs) in the IL-10 gene promoter region (rs1800890, rs1800896, rs1800871), IL-12B (rs2546890), IL-12RB1 (rs372889), IL-17A (rs2275913), and IL-23R (rs11209026) affect the immune responses after acellular pertussis vaccination. The T cell proliferative response was evaluated in 38 Finnish young adults who received a second booster dose of a vaccine combination of diphtheria, tetanus, and acellular pertussis, 10 years after the previous booster. The response was evaluated with a proliferation assay in which vaccine antigens pertussis toxin (PT), filamentous hemagglutinin (FHA), and pertactin (PRN) were used for the stimulation, before and 1 month after the second vaccination. Specific proliferation of peripheral blood mononuclear cells against pertussis antigens was affected by IL-10 SNP in the promoter region at position -1082 (A>G, rs1800896). One month after the vaccination, subjects with the AA and AG genotypes had a significantly higher T cell proliferative response against PT and FHA compared to those with the GG genotype. Subjects with the GG genotype had the lowest responses. As a conclusion, our preliminary results indicate that IL-10 SNP -1082 might play an important role in T cell-mediated immune responses after acellular pertussis vaccination.
Department of Medical Microbiology and Immunology, University of Turku, Turku, Finland.|Department of Infectious Disease Surveillance and Control, National Institute for Health and Welfare, Turku, Finland.
Kirsi
K
Gröndahl-Yli-Hannuksela
Department of Biostatistics, University of Turku, Turku, Finland.
Tero
T
Vahlberg
Immunogenetics Laboratory, University of Turku, Turku, Finland.
Jorma
J
Ilonen
Department of Pediatrics and Adolescent Medicine, Turku University Hospital, Turku, Finland.
Jussi
J
Mertsola
Department of Medical Microbiology and Immunology, University of Turku, Turku, Finland. qiushui.he@utu.fi.|Department of Infectious Disease Surveillance and Control, National Institute for Health and Welfare, Turku, Finland. qiushui.he@utu.fi.|Department of Medical Microbiology, Capital Medical University, Beijing, China. qiushui.he@utu.fi.
Qiushui
Q
He
D000293:Adolescent; D001886:Bordetella pertussis / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D002648:Child; D015331:Cohort Studies; D005260:Female; D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology; D016753:Interleukin-10 / Q000235:genetics*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D011110:Polymorphism, Genetic / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D013601:T-Lymphocytes / Q000276:immunology*; D022282:Vaccines, Acellular / Q000008:administration & dosage / Q000276:immunology*; D014917:Whooping Cough / Q000235:genetics* / Q000276:immunology / Q000517:prevention & control; D055815:Young Adult
C508609:IL10 protein, human; D022282:Vaccines, Acellular; D016753:Interleukin-10
Cell-mediated immunity; IL-10; Pertussis; Single nucleotide polymorphism; Vaccine