Production of mycelial biomass by the Amazonian edible mushroom Pleurotus albidus.
Edible mushroom species are considered as an adequate source of food in a healthy diet due to high content of protein, fiber, vitamins, and a variety of minerals. The representatives of Pleurotus genus are characterized by distinct gastronomic, nutritional, and medicinal properties among the edible mushrooms commercialized worldwide. In the present study, the growth of mycelial biomass of Pleurotus albidus cultivated in submerged fermentation was evaluated. Saccharose, fructose, and maltose were the three main carbon sources for mycelial biomass formation with corresponding yields of 7.28gL(-1), 7.07gL(-1), and 6.99gL(-1). Inorganic nitrogen sources did not stimulate growth and the optimal yield was significantly higher with yeast extract (7.98gL(-1)). The factorial design used to evaluate the influence of saccharose and yeast extract concentration, agitation speed, and initial pH indicated that all variables significantly influenced the production of biomass, especially the concentration of saccharose. The greater amount of saccharose resulted in the production of significantly more biomass. The highest mycelial biomass production (9.81gL(-1)) was reached in the medium formulated with 30.0gL(-1) saccharose, 2.5gL(-1) yeast extract, pH 7.0, and a speed of agitation at 180rpm. Furthermore, P. albidus manifested different aspects of morphology and physiology under the growth conditions employed. Media composition affected mycelial biomass production indicating that the diversification of carbon sources promoted its improvement and can be used as food or supplement.
Culture Collection DPUA (Parasitology Department of Amazonas University) / Federal University of Amazonas, Manaus, Amazonas, Brazil.
Larissa de Souza
Lde S
Kirsch
Culture Collection DPUA (Parasitology Department of Amazonas University) / Federal University of Amazonas, Manaus, Amazonas, Brazil.
Ana Júlia Porto
AJ
de Macedo
Culture Collection DPUA (Parasitology Department of Amazonas University) / Federal University of Amazonas, Manaus, Amazonas, Brazil. Electronic address: mteixeira@ufam.edu.br.
Maria Francisca Simas
MF
Teixeira
D018533:Biomass; D050260:Carbohydrate Metabolism; D002244:Carbon / Q000378:metabolism; D005285:Fermentation; D025282:Mycelium; D009584:Nitrogen / Q000378:metabolism; D020076:Pleurotus / Q000502:physiology* / Q000648:ultrastructure
D002244:Carbon; D009584:Nitrogen
Factorial design; Mycelial biomass; Pleurotus albidus; Submerged fermentation