Finite-difference time-domain-based optical microscopy simulation of dispersive media facilitates the development of optical imaging techniques.
Combining finite-difference time-domain (FDTD) methods and modeling of optical microscopy modalities, we previously developed an open-source software package called Angora, which is essentially a “microscope in a computer.” However, the samples being simulated were limited to nondispersive media. Since media dispersions are common in biological samples (such as cells with staining and metallic biomarkers), we have further developed a module in Angora to simulate samples having complicated dispersion properties, thereby allowing the synthesis of microscope images of most biological samples. We first describe a method to integrate media dispersion into FDTD, and we validate the corresponding Angora dispersion module by applying Mie theory, as well as by experimentally imaging gold microspheres. Then, we demonstrate how Angora can facilitate the development of optical imaging techniques with a case study.
Northwestern University, Department of Biomedical Engineering, 2145 Sheridan Road, Evanston, Illinois 60208, United States.
Di
D
Zhang
Northwestern University, Department of Biomedical Engineering, 2145 Sheridan Road, Evanston, Illinois 60208, United States.
Ilker
I
Capoglu
Northwestern University, Applied Physics Program, 2145 Sheridan Road, Evanston, Illinois 60208, United States.
Yue
Y
Li
Northwestern University, Department of Biomedical Engineering, 2145 Sheridan Road, Evanston, Illinois 60208, United States.
Lusik
L
Cherkezyan
Northwestern University, Department of Biomedical Engineering, 2145 Sheridan Road, Evanston, Illinois 60208, United States.
John
J
Chandler
Northwestern University, Department of Chemical and Biological Engineering, 2145 Sheridan Road, Evanston, Illinois 60208, United States.
Graham
G
Spicer
Northwestern University, Department of Biomedical Engineering, 2145 Sheridan Road, Evanston, Illinois 60208, United States.
Hariharan
H
Subramanian
Northwestern University, Department of Electrical Engineering and Computer Science, 2145 Sheridan Road, Evanston, Illinois 60208, United States.
Allen
A
Taflove
Northwestern University, Department of Biomedical Engineering, 2145 Sheridan Road, Evanston, Illinois 60208, United States.
Vadim
V
Backman
D000818:Animals; D003198:Computer Simulation; D006046:Gold / Q000737:chemistry*; D008853:Microscopy; D008863:Microspheres; D061848:Optical Imaging / Q000379:methods*; D011817:Rabbits; D013997:Time Factors
D006046:Gold