Population and life-stage-specific effects of two herbicide formulations on the aquatic development of European common frogs (Rana temporaria).
Environmental contamination is suggested to contribute to amphibian population declines. However, the effects of a contaminant on a particular amphibian species can differ among populations. The authors investigated the toxic effects of 2 herbicide formulations on different populations and on representative developmental stages of the European common frog (Rana temporaria). Larvae from forest populations were more sensitive to a commonly used glyphosate-based herbicide compared with individuals from agrarian land. Median lethal concentrations correlated with measured glyphosate levels in the breeding ponds, which may be a sign of evolved tolerances. The reverse result was observed for a less commonly used cycloxydim-based herbicide. Effects of the glyphosate-based herbicide were stronger for earlier larval stages compared with later larval stages. Hence, applications in early spring (when early larvae are present in breeding ponds) pose greater risk concerning acute toxic effects on R. temporaria. With regard to late larval stages, short exposure (96 h) of prometamorphic larvae prolonged time to metamorphosis, but only at the highest test concentration that did not significantly induce mortality. This could be due to impairment of the thyroid axis. Notably, nearly all test concentrations of the 2 herbicides provoked growth retardation. Further research on how evolved or induced tolerances are acquired, actual contamination levels of amphibian habitats, and potential endocrine effects of glyphosate-based herbicides is necessary. Environ Toxicol Chem 2017;36:190-200. © 2016 SETAC.
Trier University, Department of Biogeography, Trier, Germany.
Norman
N
Wagner
Trier University, Department of Biogeography, Trier, Germany.
Michael
M
Veith
Trier University, Department of Biogeography, Trier, Germany.
Stefan
S
Lötters
Trier University, Department of Biogeography, Trier, Germany.
Bruno
B
Viertel
D000818:Animals; D003510:Cyclohexanes / Q000737:chemistry / Q000633:toxicity; D017753:Ecosystem; D004784:Environmental Monitoring / Q000379:methods*; D005858:Germany; D005998:Glycine / Q000031:analogs & derivatives / Q000737:chemistry / Q000633:toxicity; D006540:Herbicides / Q000737:chemistry / Q000633:toxicity*; D007814:Larva / Q000187:drug effects* / Q000254:growth & development; D008675:Metamorphosis, Biological / Q000187:drug effects*; D011714:Pyrans / Q000737:chemistry / Q000633:toxicity; D011896:Rana temporaria; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000633:toxicity*; D000097797:Glyphosate
D003510:Cyclohexanes; D005998:Glycine; D006540:Herbicides; D011714:Pyrans; D014874:Water Pollutants, Chemical; D000097797:Glyphosate; C401281:cycloxydim
Amphibian; Anura; Cycloxydim; Focus® Ultra; Glyphosate; Pesticide; Roundup® UltraMax