mRNA Profiling for Vaginal Fluid and Menstrual Blood Identification.
Recently mRNA profiling has been widely proposed as a universal tool for biological fluids identification. Here, we describe a test for vaginal fluid identification that combines detection of five markers: vaginal mRNAs and Lactobacilli in end-point PCR reaction. The test detects the following transcripts: HBD1 (Human beta-defensin 1), MUC4 (Mucin 4), MMP11 (Matrix metalloproteinase 11), housekeeping gene G6PDH (glucose 6-phosphate dehydrogenase), and the 16S-23S rRNA intergenic spacer regions of L. crispatus and L. gasseri/L. johnsonii. Simultaneous analysis of five vaginal markers and a housekeeping gene ensures high specificity and reliability in the detection of vaginal material, which could not be obtained using detection of a single marker.
Department of Forensic Medicine, Laboratory of Forensic Biology and Genetics, Medical University of Gdansk, ul.Dębowa 23, Gdańsk, 80-204, Poland. jjakubowska@gumed.edu.pl.
Joanna
J
Jakubowska
Department of Forensic Medicine, Laboratory of Forensic Biology and Genetics, Medical University of Gdansk, ul.Dębowa 23, Gdańsk, 80-204, Poland.
Agnieszka
A
Maciejewska
Department of Forensic Medicine, Laboratory of Forensic Biology and Genetics, Medical University of Gdansk, ul.Dębowa 23, Gdańsk, 80-204, Poland.
Ryszard
R
Pawłowski
D015415:Biomarkers / Q000378:metabolism*; D001769:Blood; D001826:Body Fluids / Q000737:chemistry*; D005260:Female; D053803:Forensic Genetics; D006801:Humans; D008598:Menstruation; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics*; D015203:Reproducibility of Results; D017423:Sequence Analysis, RNA / Q000379:methods*; D014621:Vagina
D015415:Biomarkers; D012333:RNA, Messenger
Forensic genetics; Lactobacillus sp.; Menstrual blood; Multiplex PCR; Vaginal fluid; mRNA profiling