Before In Vivo Imaging: Evaluation of Fluorescent Probes Using Fluorescence Microscopy, Multiplate Reader, and Cytotoxicity Assays.
Fluorescent probes are widely utilized for noninvasive fluorescence imaging. Continuing efforts have been made in developing novel fluorescent probes with improved fluorescence quantum yield, enhanced target-specificity, and lower cytotoxicity. Before such probes are administrated into a living system, it is essential to evaluate the subcellular uptake, targeting specificity, and cytotoxicity in vitro. In this chapter, we briefly outline common methods used to evaluate fluorescent probes using fluorescence microscopy, multiplate reader, and cytotoxicity assay.
Molecular Imaging Lab, Department of Radiology, University of Pittsburgh, Suite 452G, 100 Technology Drive, Pittsburgh, PA, 15219, USA. zhangs2@upmc.edu.
Shaojuan
S
Zhang
D005456:Fluorescent Dyes / Q000032:analysis* / Q000493:pharmacokinetics / Q000633:toxicity; D008856:Microscopy, Fluorescence; D057054:Molecular Imaging / Q000379:methods*; D011789:Quantum Theory; D018675:Toxicity Tests
D005456:Fluorescent Dyes
Cytotoxicity; Fluorescence microscopy; Multiplate reader; Targeting specificity