A versatile quantitation platform based on platinum nanoparticles incorporated volumetric bar-chart chip for highly sensitive assays.
Platinum nanoparticles incorporated volumetric bar-chart chip (PtNPs-V-Chip) is able to be used for point-of-care tests by providing quantitative and visualized readout without any assistance from instruments, data processing, or graphic plotting. To improve the sensitivity of PtNPs-V-Chip, hybridization chain reaction was employed in this quantitation platform for highly sensitive assays that can detect as low as 16 pM Ebola Virus DNA, 0.01ng/mL carcinoembryonic antigen (CEA), and the 10 HER2-expressing cancer cells. Based on this amplified strategy, a 100-fold decrease of detection limit was achieved for DNA by improving the number of platinum nanoparticle catalyst for the captured analyte. This quantitation platform can also distinguish single base mismatch of DNA hybridization and observe the concentration threshold of CEA. The new strategy lays the foundation for this quantitation platform to be applied in forensic analysis, biothreat detection, clinical diagnostics and drug screening.
Department of Biomedical Engineering, College of Engineering and Applied Sciences, Nanjing University, Nanjing, Jiangsu 210093, China; Department of Nanomedicine, Houston Methodist Research Institute, 6670 Bertner Ave, Houston, TX 77030, USA; Department of Cell and Developmental Biology, Weill Medical College of Cornell University, New York, NY 10065, USA.
Yuzhen
Y
Wang
Department of Nanomedicine, Houston Methodist Research Institute, 6670 Bertner Ave, Houston, TX 77030, USA; Department of Cell and Developmental Biology, Weill Medical College of Cornell University, New York, NY 10065, USA.
Guixian
G
Zhu
Department of Obstetrics & Gynecology, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 660032, China. Electronic address: wenjincookie@163.com.
Wenjin
W
Qi
Department of Nanomedicine, Houston Methodist Research Institute, 6670 Bertner Ave, Houston, TX 77030, USA; Department of Cell and Developmental Biology, Weill Medical College of Cornell University, New York, NY 10065, USA.
Ying
Y
Li
Department of Biomedical Engineering, College of Engineering and Applied Sciences, Nanjing University, Nanjing, Jiangsu 210093, China. Electronic address: ysong@nju.edu.cn.
Yujun
Y
Song
D001940:Breast / Q000473:pathology; D001943:Breast Neoplasms / Q000175:diagnosis / Q000473:pathology; D002272:Carcinoembryonic Antigen / Q000097:blood*; D045744:Cell Line, Tumor; D004279:DNA, Viral / Q000032:analysis* / Q000097:blood; D029043:Ebolavirus / Q000302:isolation & purification*; D004867:Equipment Design; D005260:Female; D019142:Hemorrhagic Fever, Ebola / Q000097:blood / Q000175:diagnosis / Q000821:virology; D006801:Humans; D056656:Lab-On-A-Chip Devices; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010984:Platinum / Q000737:chemistry*; D019095:Point-of-Care Systems; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000032:analysis*
D002272:Carcinoembryonic Antigen; D004279:DNA, Viral; D010984:Platinum; D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human
Catalase-like activity; Microfluidic; Platinum nanoparticle; Point-of-care test; Signal amplification