Analysis of the metatranscriptome of microbial communities of an alkaline hot sulfur spring revealed different gene encoding pathway enzymes associated with energy metabolism.
Alkaline sulfur hot springs notable for their specialized and complex ecosystem powered by geothermal energy are abundantly rich in different chemotrophic and phototrophic thermophilic microorganisms. Survival and adaptation of these organisms in the extreme environment is specifically related to energy metabolism. To gain a better understanding of survival mechanism of the organisms in these ecosystems, we determined the different gene encoding enzymes associated with anaerobic pathways of energy metabolism by applying the metatranscriptomics approach. The analysis of the microbial population of hot sulfur spring revealed the presence of both aerobic and anaerobic organisms indicating dual mode of lifestyle of the community members. Proteobacteria (28.1 %) was the most dominant community. A total of 988 reads were associated with energy metabolism, out of which 33.7 % of the reads were assigned to nitrogen, sulfur, and methane metabolism based on KEGG classification. The major lineages of hot spring communities were linked with the anaerobic pathways. Different gene encoding enzymes (hao, nir, nar, cysH, cysI, acs) showed the involvement of microbial members in nitrification, denitrification, dissimilatory sulfate reduction, and methane generation. This study enhances our understanding of important gene encoding enzymes involved in energy metabolism, required for the survival and adaptation of microbial communities in the hot spring.
Environmental Microbiology Unit, Division of Fish Health Management, Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, 751002, Odisha, India.
Swetaleena
S
Tripathy
Environmental Microbiology Unit, Division of Fish Health Management, Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, 751002, Odisha, India.
Soumesh Kumar
SK
Padhi
Environmental Microbiology Unit, Division of Fish Health Management, Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, 751002, Odisha, India.
Sriprakash
S
Mohanty
Environmental Microbiology Unit, Division of Fish Health Management, Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, 751002, Odisha, India.
Mrinal
M
Samanta
Environmental Microbiology Unit, Division of Fish Health Management, Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar, 751002, Odisha, India. maitink@yahoo.co.in.
Nikhil Kumar
NK
Maiti
D000468:Alkalies / Q000032:analysis; D001421:Bacteria, Anaerobic / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004734:Energy Metabolism; D045482:Hot Springs / Q000737:chemistry / Q000382:microbiology*; D008697:Methane / Q000378:metabolism; D064307:Microbiota; D009584:Nitrogen / Q000378:metabolism; D020560:Proteobacteria / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013455:Sulfur / Q000032:analysis / Q000378:metabolism*; D059467:Transcriptome
D000468:Alkalies; D001426:Bacterial Proteins; D013455:Sulfur; D009584:Nitrogen; D008697:Methane
Energy metabolism; Functional pathways; Hot spring; Microbial communities