Extraction, characterization and antioxidant activity of water-soluble polysaccharides from Tuber huidongense.
Single-factor experiment and Box-Behnken design were employed to optimize the ultrasonic-assisted extraction (UAE) of water-soluble polysaccharides from Chinese truffle Tuber huidongense (THWP). The optimal extraction conditions with an extraction yield of 7.17±0.22% crude THWP were determined as follows: ultrasonic power 99.65W, extraction time 40.39min, ratio of water to raw material 24.65mL/g, and extraction temperature 70.1°C. Two purified fractions, THWP-1 and THWP-2 with molecular weights of 128kDa and 729kDa, respectively, were obtained from crude THWP by Cellulose DEAE-52 and Sephadex G-100 chromatography. Monosaccharide component analysis by high performance liquid chromatography (HPLC) indicated that THWP-1 was composed of Glc, Man, and Gal with their corresponding mole percentages of 60.56%, 20.12% and 19.32%, respectively and that THWP-2 contained only Glc. Evaluation of the antioxidant activity in vitro suggested that THWP-1 and THWP-2 had effective scavenging activity of 2,2'-azinobis-(3-ethylbenzothiazolin-6-sulfonicacid) (ABST), hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. Accordingly, the polysaccharides from Chinese truffle might serve as a natural antioxidant in functional foods.
College of Food Science, Southwest University, 2 Tiansheng Road, Beibei, Chongqing 400715, PR China; College of Life Science, Guizhou Normal University, 160 Baoshanbei Road, Guiyang 550001, PR China.
Guangjing
G
Chen
College of Food Science, Southwest University, 2 Tiansheng Road, Beibei, Chongqing 400715, PR China.
Shiqi
S
Zhang
Department of medical technology, Chongqing three gorges medical college, 366 Tianxing Road, Wanzhou, Chongqing 404120, PR China.
Chunxia
C
Ran
College of Food Science, Southwest University, 2 Tiansheng Road, Beibei, Chongqing 400715, PR China.
Lisha
L
Wang
College of Food Science, Southwest University, 2 Tiansheng Road, Beibei, Chongqing 400715, PR China. Electronic address: kanjianquan@163.com.
Jianquan
J
Kan
D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification; D001203:Ascomycota / Q000737:chemistry*; D062610:Fungal Polysaccharides / Q000737:chemistry / Q000302:isolation & purification; D012995:Solubility; D014867:Water / Q000737:chemistry
D000975:Antioxidants; D062610:Fungal Polysaccharides; D014867:Water
Antioxidant activity; Characterization; Polysaccharide; Tuber huidongense; Ultrasonic-assisted extraction