Microarray-based long oligonucleotides probe designed for Brucella Spp. detection and identification of antibiotic susceptibility pattern.
Brucella spp. is a common zoonotic infection referred to as Brucellosis, and it is a serious public health problem around the world. There are currently six classical species (pathogenic species in both animals and humans) within the genus Brucella. The ability and practicality facilitated by a microarray experiment help us to recognize Brucella spp. and its antibiotic resistant gene. Rapid phenotypic determination of antibiotic resistance is not possible by disk diffusion methods. Thus, evaluating antibiotics pattern and Brucella detection appear necessary technique by molecular methods in brucellosis. So, the aim of this study was to design a microarray long oligonucleotides probe and primer for the complete diagnosis of Brucella spp. and obtaining genetic profiles for antibiotic resistance in bacteria at the same time. In this study, we designed 16 antibiotic-resistant gene solid-phase primers with similar melting temperatures of 60 °C and 16 long oligonucleotide probes. These primers and probes can identify tetracycline-, chloramphenicol-, and aminoglycoside-resistant genes, respectively. The design of microarray probes is a versatile process that be done in a wide range of selections. Since the long oligo microarray probes are the best choices for specific diagnosis and definite treatment, this group of probes was designed in the present survey.
M.Sc. of Biotechnology, Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Zahra
Z
Khazaei
Ph.D. of Bioinformatic, Assistance Professor, Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Ali
A
Najafi
M.Sc. of Microbiology, Department of Biology, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran.
Vahhab
V
Piranfar
Ph.D. of Medical Bacteriology, Associate Professor, Molecular Biology Research Centre, Baqiyatallah University of Medical Sciences Tehran. Iran.
Reza
R
Mirnejad
Brucella; Microarray; antibiotic susceptibility; detection