Characterization of alginate-brushite in-situ hydrogel composites.
In the present study alginate-brushite composite hydrogels were in-situ synthetized and characterized with respect to preparation parameters. Specifically, the influence of initial pH value and initial concentration of phosphate precursor on the in-situ fabrication of the composite hydrogel were taken into account. The composite hydrogels were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric (TGA, DTG) and differential thermal analysis (DTA). Finally, the cell viability tests were carried out (MTT) over the incubation time period of 3, 7, and 14days. The results revealed that the formation and the crystalline stability of brushite were highly dependent on the initial pH value. It was shown that as the pH reached to the value of 6, characteristics peaks of brushite appeared in the FTIR spectra. Besides, the XRD and thermal analysis results were in a good accordance with those of FTIR. In addition, the SEM images demonstrated that the plate like brushite was formed inside the alginate matrix. Also, a considerable impact of pH variation on the biocompatibility of samples was noticed so that the majority of samples especially those prepared in the acidic conditions were toxic.
Department of Informatics, Bioengineering, Robotics, and System Engineering, University of Genoa, Genoa, Italy.
Seyed Mohammad Hossein
SMH
Dabiri
Department of Civil, Chemical and Environmental Engineering, University of Genoa, Genoa, Italy.
Alberto
A
Lagazzo
Department of Civil, Chemical and Environmental Engineering, University of Genoa, Genoa, Italy.
Fabrizio
F
Barberis
National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.
Mehdi
M
Farokhi
Department of Civil, Chemical and Environmental Engineering, University of Genoa, Genoa, Italy.
Elisabetta
E
Finochio
Department of Informatics, Bioengineering, Robotics, and System Engineering, University of Genoa, Genoa, Italy.
Laura
L
Pastorino
D000464:Alginates / Q000737:chemistry / Q000494:pharmacology; D002130:Calcium Phosphates / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D020723:Glucuronic Acid / Q000737:chemistry / Q000494:pharmacology; D006603:Hexuronic Acids / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D020100:Hydrogels / Q000737:chemistry / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration
D000464:Alginates; D002130:Calcium Phosphates; D006603:Hexuronic Acids; D020100:Hydrogels; D020723:Glucuronic Acid; C494366:calcium phosphate, dibasic, dihydrate
Alginate; Biocompatibility; Brushite; Hydrogel; In-situ composite; Physical characterization