Maintenance of High Cytochrome P450 Expression in HepaRG Cell Spheroids in DMSO-Free Medium.
We studied the effects of DMSO and fibroblasts during HepaRG cell spheroid formation and conditions of their subsequent culturing on the levels of mRNA of the major cytochromes P450. A protocol of spheroid formation from differentiated HepaRG cells and their culturing in serum- and DMSO-free medium is developed.
BioClinicum Research and Production Center, Moscow, Russia. a.aleksandrova@bioclinicum.com.
A V
AV
Aleksandrova
BioClinicum Research and Production Center, Moscow, Russia.
O A
OA
Burmistrova
National Medical Research Radiology Center, Moscow, Russia.
K A
KA
Fomicheva
BioClinicum Research and Production Center, Moscow, Russia.|Moscow State University of Mechanical Engineering (MAMI), Moscow, Russia.
D A
DA
Sakharov
D002460:Cell Line; D003470:Culture Media / Q000737:chemistry; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D015870:Gene Expression; D015971:Gene Expression Regulation, Enzymologic; D022781:Hepatocytes / Q000201:enzymology*; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018874:Spheroids, Cellular / Q000201:enzymology*
D003470:Culture Media; D012333:RNA, Messenger; D003577:Cytochrome P-450 Enzyme System; D004121:Dimethyl Sulfoxide
HepaRG; cytochromes P450; differentiation