Effect and mechanism of miR-34a on proliferation, apoptosis and invasion of laryngeal carcinoma cells.
OBJECTIVE
To discuss the effect and mechanism of miR-34a on the proliferation, apoptosis and invasion of laryngeal carcinoma cells.


METHODS
The laryngeal squamous carcinoma Hep2 cells were transiently transfected with miR-34a mimics and miR-34a NC. The MTT, colony-forming assay, Hoechst staining and AnnexinV-PI double staining flow cytometry were employed to detect the effect of miR-34a on the viability and apoptosis of laryngeal squamous carcinoma Hep2 cells; Transwell assay to defect the effect of miR-34a on the migration and invasion of laryngeal squamous carcinoma Hep2 cells; western blot and RT-PCR assay to defect the effect of miR-34a mimics on the expression of survivin and Ki-67 mRNA in laryngeal squamous carcinoma Hep2 cells.


RESULTS
Compared with miR-34a NC group, the cell viability in miR-34 mimics group was significantly decreased (P < 0.01), the cell apoptosis rate was significantly increased (P < 0.01), the abilities of cell migration and invasion were significantly reduced (P < 0.01) and the expression of survivin and Ki-67 mRNA was significantly decreased (P < 0.01).


CONCLUSIONS
The increased expression of miR-34a can induce the apoptosis of Hep2 laryngeal carcinoma cells and inhibit the cell proliferation and invasion, which is related to the down-regulated expression of survivin and Ki-67.
Affiliated Hospital of Hebei University of Engineering, Handan 056002, Hebei, China.
Ju-Xiang
JX
Wang
Affiliated Hospital of Hebei University of Engineering, Handan 056002, Hebei, China. Electronic address: entzqj@qq.com.
Qing-Jun
QJ
Zhang
Affiliated Hospital of Hebei University of Engineering, Handan 056002, Hebei, China.
Shi-Geng
SG
Pei
Affiliated Hospital of Hebei University of Engineering, Handan 056002, Hebei, China.
Bao-Liang
BL
Yang
Apoptosis; Invasion; Laryngeal squamous carcinoma Hep2 cells; MiR-34a; Proliferation