Intron Derived Size Polymorphism in the Mitochondrial Genomes of Closely Related Chrysoporthe Species.
In this study, the complete mitochondrial (mt) genomes of Chrysoporthe austroafricana (190,834 bp), C. cubensis (89,084 bp) and C. deuterocubensis (124,412 bp) were determined. Additionally, the mitochondrial genome of another member of the Cryphonectriaceae, namely Cryphonectria parasitica (158,902 bp), was retrieved and annotated for comparative purposes. These genomes showed high levels of synteny, especially in regions including genes involved in oxidative phosphorylation and electron transfer, unique open reading frames (uORFs), ribosomal RNAs (rRNAs) and transfer RNAs (tRNAs), as well as intron positions. Comparative analyses revealed signatures of duplication events, intron number and length variation, and varying intronic ORFs which highlighted the genetic diversity of mt genomes among the Cryphonectriaceae. These mt genomes showed remarkable size polymorphism. The size polymorphism in the mt genomes of these closely related Chrysoporthe species was attributed to the varying number and length of introns, coding sequences and to a lesser extent, intergenic sequences. Compared to publicly available fungal mt genomes, the C. austroafricana mt genome is the second largest in the Ascomycetes thus far.
Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Private bag x20, Pretoria, 0028, South Africa.
Aquillah Mumo
AM
Kanzi
Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Private bag x20, Pretoria, 0028, South Africa.
Brenda Diana
BD
Wingfield
Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Private bag x20, Pretoria, 0028, South Africa.
Emma Theodora
ET
Steenkamp
Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Private bag x20, Pretoria, 0028, South Africa.
Sanushka
S
Naidoo
Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Private bag x20, Pretoria, 0028, South Africa.
Nicolaas Albertus
NA
van der Merwe
D001203:Ascomycota / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D021901:DNA, Intergenic; D004272:DNA, Mitochondrial / Q000235:genetics; D019143:Evolution, Molecular; D023061:Gene Order; D050259:Genes, Mitochondrial; D014644:Genetic Variation; D016681:Genome, Fungal; D054629:Genome, Mitochondrial; D007438:Introns / Q000235:genetics*; D058977:Molecular Sequence Annotation; D010802:Phylogeny; D011110:Polymorphism, Genetic
D021901:DNA, Intergenic; D004272:DNA, Mitochondrial