Application of Laser Correlation Spectroscopy for Measuring Virus Size.
Dynamic light scattering method or laser correlation spectroscopy was applied to evaluation of the size of viruses. We measured correlation functions of the light scattered by human immunodeficiency viruses (HIV) and hepatitis A viruses (HAV) and found that size of HIV-1 (subtype A and B) and HAV virions were 104 nm and 28 nm, respectively. Comparison of these findings with electron microscopy data for fixed samples of the same viruses showed good agreement of the results.
Physical Faculty, M. V. Lomonosov Moscow State University, Moscow, Russia. nvn@lt.phys.msu.ru.
V N
VN
Nikiforov
N. N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow, Russia.
S E
SE
Vinogradov
N. N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Moscow, Russia.
A V
AV
Ivanov
D. I. Ivanovsky Institute of Virology, N. F. Gamaleya Federal Center of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia.
E V
EV
Efremova
D. I. Ivanovsky Institute of Virology, N. F. Gamaleya Federal Center of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia.
L B
LB
Kalnina
M. P. Chumakov Institute of Poliomyelitis and Viral Encephalitides, Moscow, Russia.
A B
AB
Bychenko
D. I. Ivanovsky Institute of Virology, N. F. Gamaleya Federal Center of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia.
Yu Yu
YY
Tentsov
D. I. Ivanovsky Institute of Virology, N. F. Gamaleya Federal Center of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia.
A A
AA
Manykin
D002460:Cell Line; D000067493:Dynamic Light Scattering; D015497:HIV-1 / Q000648:ultrastructure*; D030041:Hepatitis A virus / Q000648:ultrastructure*; D006801:Humans; D007834:Lasers; D008854:Microscopy, Electron; D010316:Particle Size; D013057:Spectrum Analysis
HIV-1; electron microscopy; hepatitis A virus; laser correlation spectroscopy (dynamic light scattering); virus size