Evidence of multiple reassortment events of feline-to-human rotaviruses based on a rare human G3P[9] rotavirus isolated from a patient with acute gastroenteritis.
A rare human/feline-like rotavirus G3P[9] strain, CAU14-1-262, from a 2-year-old girl with severe gastroenteritis was isolated and sequenced. The 11 gene segments of the CAU14-1-262 strain possessed a novel genotype constellation, G3-P[9]-I3-R3-C3-M3-A3-N3-T1-E3-H6, which was identified for the first time. Phylogenetic analysis of this strain identified the following genome origins: VP7, VP4, VP6, VP1-VP3, NSP1, NSP2, and NSP4 genes possessed an AU-1-like genotype 3 constellation with high sequence identity to those of the feline and human/feline-like rotaviruses; NSP5 possessed a H6 lineage, with highest sequence identity to the human/feline-like E2541 strain; and the NSP3 gene possessed a Wa-like genotype 1 constellation with high sequence identity to those of the of human rotaviruses. These results provided evidence of multiple reassortment events in G3P[9] rotavirus CAU14-1-262 and possibility of feline-to-human interspecies transmission.
Department of Microbiology, Chung-Ang University College of Medicine, Seoul, South Korea.
Tinh Huu
TH
Nguyen
Department of Microbiology, Chung-Ang University College of Medicine, Seoul, South Korea.
Van Thai
VT
Than
Department of Microbiology, Chung-Ang University College of Medicine, Seoul, South Korea.
Hien Dang
HD
Thanh
Department of Microbiology, Chung-Ang University College of Medicine, Seoul, South Korea. Electronic address: kimwy@cau.ac.kr.
Wonyong
W
Kim
D000208:Acute Disease; D000818:Animals; D002415:Cats; D002675:Child, Preschool; D005243:Feces / Q000821:virology; D005260:Female; D005759:Gastroenteritis / Q000821:virology*; D016679:Genome, Viral; D005838:Genotype; D006801:Humans; D010802:Phylogeny; D012367:RNA, Viral; D016865:Reassortant Viruses / Q000235:genetics* / Q000302:isolation & purification* / Q000502:physiology; D012401:Rotavirus / Q000235:genetics* / Q000302:isolation & purification* / Q000502:physiology; D012400:Rotavirus Infections / Q000635:transmission / Q000821:virology*; D017422:Sequence Analysis, DNA
D012367:RNA, Viral
Feline; G3P[9]; Human; Interspecies; Transmission