Ultrasensitive detection of inhaled organic aerosol particles by accelerator mass spectrometry.
Accelerator mass spectrometry (AMS) was shown to be applicable for studying the penetration of organic aerosols, inhaled by laboratory mice at ultra-low concentration ca. 10(3) cm(-3). We synthesized polystyrene (PS) beads, composed of radiocarbon-labeled styrene, for testing them as model organic aerosols. As a source of radiocarbon we used methyl alcohol with radioactivity. Radiolabeled polystyrene beads were obtained by emulsifier-free emulsion polymerization of synthesized (14)C-styrene initiated by K2S2O8 in aqueous media. Aerosol particles were produced by pneumatic spraying of diluted (14)C-PS latex. Mice inhaled (14)C-PS aerosol consisting of the mix of 10(3) 225-nm particles per 1 cm(3) and 5·10(3) 25-nm particles per 1 cm(3) for 30 min every day during five days. Several millions of 225-nm particles deposited in the lungs and slowly excreted from them during two weeks of postexposure. Penetration of particles matter was also observed for liver, kidneys and brain, but not for a heart.
Novosibirsk State University, Laboratory of Radiocarbon Methods of Analyses, 2 Pirogova st., Novosibirsk, 630090, Russia; Boreskov Institute of Catalysis SB RAS, 5 Lavrentieva st., Novosibirsk, 630090, Russia. Electronic address: ekaterina@catalysis.ru.
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Parkhomchuk
Novosibirsk State University, Laboratory of Radiocarbon Methods of Analyses, 2 Pirogova st., Novosibirsk, 630090, Russia; Boreskov Institute of Catalysis SB RAS, 5 Lavrentieva st., Novosibirsk, 630090, Russia.
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Gulevich
Novosibirsk State University, Laboratory of Radiocarbon Methods of Analyses, 2 Pirogova st., Novosibirsk, 630090, Russia; N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry SB RAS, 9 Lavrentieva st., Novosibirsk, 630090, Russia.
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Taratayko
Voevodsky Institute of Chemical Kinetics and Combustion SB RAS, 3 Institutskaya st., Novosibirsk, 630090, Russia.
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Baklanov
Novosibirsk State University, Laboratory of Radiocarbon Methods of Analyses, 2 Pirogova st., Novosibirsk, 630090, Russia; JSC Tion, 20 Injenernaya str., 630090, Novosibirsk, Russia.
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Selivanova
JSC Tion, 20 Injenernaya str., 630090, Novosibirsk, Russia.
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Trubitsyna
Novosibirsk State University, Laboratory of Radiocarbon Methods of Analyses, 2 Pirogova st., Novosibirsk, 630090, Russia.
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Voronova
Novosibirsk State University, Laboratory of Radiocarbon Methods of Analyses, 2 Pirogova st., Novosibirsk, 630090, Russia; Boreskov Institute of Catalysis SB RAS, 5 Lavrentieva st., Novosibirsk, 630090, Russia.
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Kalinkin
Novosibirsk State University, Laboratory of Radiocarbon Methods of Analyses, 2 Pirogova st., Novosibirsk, 630090, Russia; Boreskov Institute of Catalysis SB RAS, 5 Lavrentieva st., Novosibirsk, 630090, Russia.
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Okunev
Novosibirsk State University, Laboratory of Radiocarbon Methods of Analyses, 2 Pirogova st., Novosibirsk, 630090, Russia; Budker Institute of Nuclear Physics SB RAS, 11 Lavrentieva st., Novosibirsk 630090, Russia.
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Rastigeev
Novosibirsk State University, Laboratory of Radiocarbon Methods of Analyses, 2 Pirogova st., Novosibirsk, 630090, Russia; N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry SB RAS, 9 Lavrentieva st., Novosibirsk, 630090, Russia.
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Reznikov
Novosibirsk State University, Laboratory of Radiocarbon Methods of Analyses, 2 Pirogova st., Novosibirsk, 630090, Russia; Boreskov Institute of Catalysis SB RAS, 5 Lavrentieva st., Novosibirsk, 630090, Russia.
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Semeykina
Novosibirsk State University, Laboratory of Radiocarbon Methods of Analyses, 2 Pirogova st., Novosibirsk, 630090, Russia; Boreskov Institute of Catalysis SB RAS, 5 Lavrentieva st., Novosibirsk, 630090, Russia.
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Sashkina
Novosibirsk State University, Laboratory of Radiocarbon Methods of Analyses, 2 Pirogova st., Novosibirsk, 630090, Russia; Budker Institute of Nuclear Physics SB RAS, 11 Lavrentieva st., Novosibirsk 630090, Russia.
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Parkhomchuk
D000280:Administration, Inhalation; D000336:Aerosols; D000818:Animals; D001921:Brain / Q000378:metabolism; D002250:Carbon Radioisotopes; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D008808:Mice, Inbred CBA; D010316:Particle Size; D011137:Polystyrenes / Q000008:administration & dosage / Q000032:analysis* / Q000493:pharmacokinetics
D000336:Aerosols; D002250:Carbon Radioisotopes; D011137:Polystyrenes
Accelerator mass-spectrometry; Low-dose inhalation; Mice; Organic aerosols; Polystyrene beads; Radiocarbon