UGT74B1 from Arabidopsis thaliana as a versatile biocatalyst for the synthesis of desulfoglycosinolates.
Thioglycosides, even if rare in Nature, have gained increased interest for their biological properties. Chemical syntheses of this class of compounds have been largely studied but little has been reported on their biosynthesis. Herein, combining experiments from the different fields of enzymology, bioorganic chemistry and molecular modeling, we wish to demonstrate the versatility of the glucosyltransferase UGT74B1 and its synthetic potency for the preparation of a variety of natural and unnatural desulfoglycosinolates.
Normandie Univ, COBRA, UMR 6014 et FR 3038; Univ Rouen; INSA Rouen; CNRS, IRCOF, 1 rue Tesnière, 76821 Mont Saint Aignan Cedex, France.
Sami
S
Marroun
Sabine
S
Montaut
Stéphanie
S
Marquès
Pierre
P
Lafite
Gaël
G
Coadou
Patrick
P
Rollin
Guillaume
G
Jousset
Marie
M
Schuler
Arnaud
A
Tatibouët
Hassan
H
Oulyadi
Richard
R
Daniellou
D017360:Arabidopsis / Q000737:chemistry* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000378:metabolism*; D055162:Biocatalysis; D005964:Glucosyltransferases / Q000737:chemistry / Q000378:metabolism*; D006027:Glycosides / Q000096:biosynthesis* / Q000737:chemistry; D015394:Molecular Structure
D029681:Arabidopsis Proteins; D006027:Glycosides; D005964:Glucosyltransferases; C499436:UDP-glucose thiohydroximate S-glucosyltransferase UGT74B1, Arabidopsis