Effect of BSA-coated Superparamagnetic Iron Oxide Nanoparticles on Granulosa Cells.
BACKGROUND
Since superparamagnetic iron oxide nanoparticles (SPION) possess unique features, they provide a huge platform for medical applications, especially for cancer diagnosis and therapy (e.g. imaging, and drug targeting). However, heterogeneous effects on mammalian cells with regard to reproductive tissue are described. An experimental study was carried out to study the effects of SPIONs on both the expression of steroid hormone receptor and viability of granulosa cells, which play a key role in ovarian health and fertility.


METHODS
Human granulosa cells were cultured in vitro and incubated with different concentrations of SPIONs. After 48 h, steroid receptor expression and cell viability were evaluated.


RESULTS
Treatment of granulosa cells with SPIONs did not affect estrogen receptor β1 or progesterone receptor-A expression and had no significant effect on cell viability.


CONCLUSIONS
Nanoparticles precoated with bovine serum albumin (BSA) do not alter granulosa cell phenotype, whereas literature suggests that other nanoparticles induce apoptosis and reduce steroid receptor expression. Our data indicate an overall better outcome using SPIONs coated with BSA.
Section for Experimental Oncology and Nanomedicine (SEON), Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital Erlangen, Erlangen, Germany.
Marina
M
Pöttler
Department of Obstetrics and Gynaecology, Ludwig Maximilian University Hospital, Munich, Germany.
Simone
S
Hofmann
Section for Experimental Oncology and Nanomedicine (SEON), Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital Erlangen, Erlangen, Germany Department of Phoniatrics and Pediatric Audiology, University Hospital Erlangen, Erlangen, Germany.
Stephan
S
Dürr
Section for Experimental Oncology and Nanomedicine (SEON), Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital Erlangen, Erlangen, Germany.
Harald
H
Unterweger
Department of Obstetrics and Gynaecology, Ludwig Maximilian University Hospital, Munich, Germany.
Irmi
I
Wiest
Section for Experimental Oncology and Nanomedicine (SEON), Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital Erlangen, Erlangen, Germany.
Jan
J
Zaloga
Section for Experimental Oncology and Nanomedicine (SEON), Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital Erlangen, Erlangen, Germany.
Christoph
C
Alexiou
Department of Obstetrics and Gynaecology, Ludwig Maximilian University Hospital, Munich, Germany.
Udo
U
Jeschke
Section for Experimental Oncology and Nanomedicine (SEON), Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital Erlangen, Erlangen, Germany christina.janko@uk-erlangen.de.
Christina
C
Janko
D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D005290:Ferric Compounds / Q000008:administration & dosage / Q000494:pharmacology*; D006107:Granulosa Cells / Q000737:chemistry / Q000187:drug effects*; D006801:Humans; D058185:Magnetite Nanoparticles / Q000008:administration & dosage*; D011960:Receptors, Estrogen / Q000032:analysis; D012710:Serum Albumin, Bovine / Q000008:administration & dosage*
D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; D011960:Receptors, Estrogen; C000499:ferric oxide; D012710:Serum Albumin, Bovine
Superparamagnetic iron oxide nanoparticles; cancer therapy and diagnosis; granulosa cells; protein corona; reproductive health