Fabrication and evaluation of nanoparticle-assembled BSA microparticles for enhanced liver delivery of glycyrrhetinic acid.
The aim of the present study was to prepare and evaluate microparticle formulation encapsulated with glycyrrhetinic acid (GA) based on bovine serum albumin (BSA). The drug-loaded nanoparticles were firstly formed by a simple desolvation method, and were further assembled into microparticles using zinc chloride and glutaraldehyde as crosslinkers. The obtained microparticles contained approximately 30% (w/w) drug and showed as spherical particles with a size of about 2 μm. Differential scanning calorimetry (DSC) and X-ray powder diffraction (XRPD) analysis indicated that GA lost its crystallinity during the nano/microencapsulation process. In vitro dissolution study demonstrated a typical sustained-release pattern for 24 h with a burst of 28.1% at the first 30 min, which fitted well by Higuchi model. After intravenous administration into mice, the microparticle formulation remained a higher drug level than the solution formulation in blood and liver for more than 18 h. These results suggested the potential benefit of using the prepared albumin microparticles as a promising vector for enhanced liver delivery of poorly water-soluble drug.
a School of Pharmacy, Ningxia Medical University , Yinchuan , Ningxia , China.|b Ningxia Engineering and Technology Research Center for Modernization of Hui Medicine , Yinchuan , Ningxia , China.|c Key Lab of Hui Ethnic Medicine Modernization, Ministry of Education , Yinchuan , Ningxia , China.
Wenping
W
Wang
a School of Pharmacy, Ningxia Medical University , Yinchuan , Ningxia , China.
Yaya
Y
Lei
a School of Pharmacy, Ningxia Medical University , Yinchuan , Ningxia , China.|b Ningxia Engineering and Technology Research Center for Modernization of Hui Medicine , Yinchuan , Ningxia , China.|c Key Lab of Hui Ethnic Medicine Modernization, Ministry of Education , Yinchuan , Ningxia , China.
Hong
H
Sui
d Department of Pharmaceutics , General Hospital of Ningxia Medical University , Yinchuan , Ningxia , China.
Wenping
W
Zhang
a School of Pharmacy, Ningxia Medical University , Yinchuan , Ningxia , China.
Rongyue
R
Zhu
a School of Pharmacy, Ningxia Medical University , Yinchuan , Ningxia , China.
Jun
J
Feng
d Department of Pharmaceutics , General Hospital of Ningxia Medical University , Yinchuan , Ningxia , China.
Hong
H
Wang
D000818:Animals; D002417:Cattle; D004337:Drug Carriers / Q000737:chemistry* / Q000493:pharmacokinetics; D065546:Drug Liberation; D006034:Glycyrrhetinic Acid / Q000737:chemistry*; D008099:Liver / Q000378:metabolism*; D051379:Mice; D008863:Microspheres; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000493:pharmacokinetics; D014018:Tissue Distribution
D004337:Drug Carriers; D012710:Serum Albumin, Bovine; D006034:Glycyrrhetinic Acid
Biodistribution; bovine serum albumin; glycyrrhetinic acid; liver targeting; microparticles; nanoparticles