Cardiac oxygen limitation during an acute thermal challenge in the European perch: effects of chronic environmental warming and experimental hyperoxia.
Oxygen supply to the heart has been hypothesized to limit cardiac performance and whole animal acute thermal tolerance (CTmax) in fish. We tested these hypotheses by continuously measuring venous oxygen tension (Pvo2) and cardiovascular variables in vivo during acute warming in European perch (Perca fluviatilis) from a reference area during summer (18°C) and a chronically heated area (Biotest enclosure) that receives warm effluent water from a nuclear power plant and is normally 5-10°C above ambient (24°C at the time of experiments). While CTmax was 2.2°C higher in Biotest compared with reference perch, the peaks in cardiac output and heart rate prior to CTmax occurred at statistically similar Pvo2 values (2.3-4.0 kPa), suggesting that cardiac failure occurred at a common critical Pvo2 threshold. Environmental hyperoxia (200% air saturation) increased Pvo2 across temperatures in reference fish, but heart rate still declined at a similar temperature. CTmax of reference fish increased slightly (by 0.9°C) in hyperoxia, but remained significantly lower than in Biotest fish despite an improved cardiac output due to an elevated stroke volume. Thus, while cardiac oxygen supply appears critical to elevate stroke volume at high temperatures, oxygen limitation may not explain the bradycardia and arrhythmia that occur prior to CTmax Acute thermal tolerance and its thermal plasticity can, therefore, only be partially attributed to cardiac failure from myocardial oxygen limitations, and likely involves limiting factors on multiple organizational levels.
Department of Biological and Environmental Sciences, University of Gothenburg, Gothenburg, Sweden; andreas.ekstrom@bioenv.gu.se.
Andreas
A
Ekström
Department of Biological and Environmental Sciences, University of Gothenburg, Gothenburg, Sweden;
Jeroen
0000-0002-3671-5038
J
Brijs
Australian Institute of Marine Science, Townsville, Queensland, Australia; and.
Timothy D
TD
Clark
Department of Animal Environment and Health, Swedish University of Agricultural Sciences, Skara, Sweden.
Albin
A
Gräns
Department of Biological and Environmental Sciences, University of Gothenburg, Gothenburg, Sweden;
Fredrik
F
Jutfelt
Department of Biological and Environmental Sciences, University of Gothenburg, Gothenburg, Sweden;
Erik
E
Sandblom
D000208:Acute Disease; D000222:Adaptation, Physiological / Q000502:physiology; D000818:Animals; D017753:Ecosystem; D057232:Global Warming; D006321:Heart / Q000502:physiology*; D006339:Heart Rate / Q000502:physiology; D018869:Heat-Shock Response / Q000502:physiology*; D009206:Myocardium / Q000378:metabolism; D010100:Oxygen / Q000097:blood*; D010471:Perches / Q000502:physiology*; D000071436:Thermotolerance / Q000502:physiology*
D010100:Oxygen
Pvo2 threshold; cardiac performance; myocardial oxygenation; teleost; thermal acclimation