The RING for gypsy moth control: Topical application of fragment of its nuclear polyhedrosis virus anti-apoptosis gene as insecticide.
Numerous studies suggest a cellular origin for the Lymantria dispar multicapsid nuclear polyhedrosis virus (LdMNPV) anti-apoptosis genes IAPs, thus opening a possibility to use the fragments of these genes for modulation of host metabolism. We report here the strong insecticidal and metabolic effect of single-stranded antisense DNA fragment from RING (really interesting new gene) domain of gypsy moth LdMNPV IAP-3 gene: specifically, on reduction of biomass (by 35%) and survival of L. dispar caterpillars. The treatment with this DNA fragment leads to a significantly higher mortality rates of female insects (1.7 fold) accompanied with the signs of apoptosis. Additionally, we show increased expression of host IAP-1, caspase-4 and gelsolin genes in eggs laid by survived females treated with RING DNA fragment accompanied with calcium and magnesium imbalance, indicating that the strong stress reactions and metabolic effects are not confined to treated insects but likely led to apoptosis in eggs too. The proposed new approach for insect pest management, which can be considered as advancement of "microbial pesticides", is based on the application of the specific virus DNA, exploiting the knowledge about virus-pest interactions and putting it to the benefit of mankind.
Vernadsky Crimean Federal University, Taurida Academy, Department of Biochemistry, Academician Vernadsky Ave., 4, 295007 Simferopol, Crimea, Ukraine. Electronic address: dna.insecticides@yahoo.com.
Volodymyr V
VV
Oberemok
Vernadsky Crimean Federal University, Medical Academy, Department of Biochemistry, Lenin Ave., 5/7, 295006 Simferopol, Crimea, Ukraine. Electronic address: botan_icus@mail.ru.
Kateryna V
KV
Laikova
Vernadsky Crimean Federal University, Taurida Academy, Department of Biochemistry, Academician Vernadsky Ave., 4, 295007 Simferopol, Crimea, Ukraine. Electronic address: zaicevfl@mail.ru.
Aleksei S
AS
Zaitsev
Lomonosov Moscow State University, Department of Virology, Moscow 119991, Russia. Electronic address: wowaniada@gmail.com.
Vladimir A
VA
Gushchin
University of Torino, Department of Oncology, via Santena 5 bis, Torino 10126, Italy. Electronic address: olexii.skorokhod@unito.it.
Oleksii A
OA
Skorokhod
D000818:Animals; D017209:Apoptosis / Q000235:genetics; D005260:Female; D005814:Genes, Viral / Q000235:genetics*; D007300:Insect Control / Q000379:methods; D007306:Insecticides; D007814:Larva; D008297:Male; D009036:Moths; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction
D007306:Insecticides
DNA insecticides; Gypsy moth Lymantria dispar; Lymantria dispar multicapsid nuclear polyhedrosis virus; Pest management; RING (really interesting new gene) domain; Viral IAP genes