Protective Effect of Antioxidant Extracts from Grey Oyster Mushroom, Pleurotus pulmonarius (Agaricomycetes), Against Human Low-Density Lipoprotein Oxidation and Aortic Endothelial Cell Damage.
This study evaluated the in vitro antioxidant capacities of extracts from Pleurotus pulmonarius via Folin-Ciocalteu, 1,1-diphenyl-2-picrylhydrazyl free radical scavenging, metal chelating, cupric ion reducing antioxidant capacity, and lipid peroxidation inhibition assays. Extract compositions were determined by phenol-sulfuric acid; Coomassie Plus (Bradford) protein; Spectroquant zinc, copper, and manganese test assays; and liquid chromatography-tandem mass spectrometry (LC/MS/MS) and gas chromatography-mass spectrometry (GC/MS). Methanol-dichloromethane extract, water fraction, hot water, aqueous extract and hexane fraction exhibited the most potent extracts in the antioxidant activities. LC/MS/MS and GC/MS showed that the extracts contained ergothioneine, ergosterol, flavonoid, and phenolic compounds. The selected potent extracts were evaluated for their inhibitory effect against oxidation of human low-density lipoproteins and protective effects against hydrogen peroxide-induced cytotoxic injury in human aortic endothelial cells. The crude aqueous extract was deemed most potent for the prevention of human low-density lipoprotein oxidation and endothelial membrane damage. Ergothioneine might be the compound responsible for the activities, as supported by previous reports. Thus, P. pulmonarius may be a valuable antioxidant ingredient in functional foods or nutraceuticals.
Faculty of Science, Institute of Biological Sciences, Mushroom Research Centre, University of Malaya, Kuala Lumpur, Malaysia.
Mohamad Hamdi Zainal
MH
Abidin
Faculty of Science, Institute of Biological Sciences, Mushroom Research Centre, University of Malaya, Kuala Lumpur, Malaysia.
Noorlidah
N
Abdullah
Faculty of Science, Institute of Biological Sciences, Mushroom Research Centre, University of Malaya, Kuala Lumpur, Malaysia.
Nurhayati Zainal
NZ
Abidin
D000975:Antioxidants / Q000302:isolation & purification / Q000378:metabolism*; D001713:Biphenyl Compounds / Q000378:metabolism; D002457:Cell Extracts / Q000737:chemistry* / Q000302:isolation & purification; D002470:Cell Survival / Q000187:drug effects; D042783:Endothelial Cells / Q000187:drug effects / Q000502:physiology*; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D015227:Lipid Peroxidation; D008077:Lipoproteins, LDL / Q000378:metabolism*; D010084:Oxidation-Reduction; D010851:Picrates / Q000378:metabolism; D020076:Pleurotus / Q000737:chemistry*
D000975:Antioxidants; D001713:Biphenyl Compounds; D002457:Cell Extracts; D016166:Free Radical Scavengers; D008077:Lipoproteins, LDL; D010851:Picrates; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl