Effect of Proline-Containing Oligopeptides PGP and RGP on Proliferative and Protein-Synthesizing Activity of Cultured Pulmonary Fibroblasts under Conditions of Oxidative Stress.
We studied the effect of glyprolines Pro-Gly-Pro (PGP) and Arg-Gly-Pro (RGP) on the primary culture of pulmonary fibroblasts from newborn albino rats under normal conditions and during oxidative stress. Under physiological conditions, the peptides had no effect on parameters of cell division. Hydrogen peroxide induced intensive oxidative stress accompanied by suppression of protein-synthesizing function. When hydrogen peroxide was added to the culture containing the test peptides, correction of the oxidative status was observed accompanied by activation of DNA-synthesizing activity and inhibition of lucigenin-dependent chemiluminescence.
Central Research Laboratory, Far-Eastern State Medical University, Khabarovsk, Russia.
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Tolstenok
Central Research Laboratory, Far-Eastern State Medical University, Khabarovsk, Russia. marfl@yandex.ru.
M Yu
MY
Fleishman
Central Research Laboratory, Far-Eastern State Medical University, Khabarovsk, Russia.
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EN
Sazonova
Central Research Laboratory, Far-Eastern State Medical University, Khabarovsk, Russia.|Research Institute of Maternal and Child Health Care, Khabarovsk Branch of Far-Eastern Research Center of Physiology and Pathology of Respiration, Khabarovsk, Russia.
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Lebed'ko
Biarritz Medical Esthetic Center, Khabarovsk, Russia.
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Maltseva
Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia.
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Myasoedov
Central Research Laboratory, Far-Eastern State Medical University, Khabarovsk, Russia.
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Timoshin
D000818:Animals; D002466:Cell Nucleolus / Q000648:ultrastructure; D049109:Cell Proliferation; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology*; D008168:Lung / Q000166:cytology; D009842:Oligopeptides / Q000494:pharmacology*; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011392:Proline / Q000031:analogs & derivatives* / Q000494:pharmacology; D014176:Protein Biosynthesis / Q000187:drug effects*; D017208:Rats, Wistar; D013481:Superoxides / Q000378:metabolism
D009842:Oligopeptides; C000608939:arginyl-glycyl-proline; C113164:prolyl-glycyl-proline; D013481:Superoxides; D011392:Proline
DNA synthesis; chemiluminescence; glyprolines; nuclear organizer; oxidative stress