Measurement of Intracellular Ionized Calcium in a Free-living Soil Nematode, Caenorhabditis elegans.
A calcium chelating fluorescence indicator, fura-2, was used to measure intracellular ionized calcium in Caenorhabditis elegans. The indicator loading process was harmless to the nematode, and completed within 2-3 h. Fura-2 was loaded mainly at its intestinal tract. The effects of DOPA on locomotion and the level of intracellular calcium were investigated and measured by using a microfluorometer. The addition of DOPA temporarily increased [Ca(2+)]i for several minutes.
a Plant Ecochemicals Project, ERATO, Research Development Corporation of Japan , 3-1-1, Megumino Kita, Eniwa-shi , Hokkaido 061-13 , Japan.
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Kawaii
a Plant Ecochemicals Project, ERATO, Research Development Corporation of Japan , 3-1-1, Megumino Kita, Eniwa-shi , Hokkaido 061-13 , Japan.
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Yoshizawa
a Plant Ecochemicals Project, ERATO, Research Development Corporation of Japan , 3-1-1, Megumino Kita, Eniwa-shi , Hokkaido 061-13 , Japan.
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Mizutani