Local pamidronate influences fracture healing in a rodent femur fracture model: an experimental study.
Bisphosphonates are a main component in the therapy of osteoporosis and other bone resorptive diseases. Previous studies have shown a positive effect of systemically applied bisphosphonates on fracture healing. Nevertheless high doses are related to side effects like osteonecrosis of the jaw, nephrotoxis and gastrointestinal symptoms. In this study we investigated the effect of locally applied pamidronate on fracture healing.



In a rodent model a simple femur fracture was set in female Wistar rats. We performed intramedullary fixation of the fracture and placed a collagen matrix around the fracture area. One group was treated with pamidronate, the other group with placebo via the matrix. To investigate the volume and quality of the callus we used micro-CT (μCT) and histology after 14 and 28 days.



Our results show a positive influence of local applied pamidronate on callus volume. After 14 days an insignificant increase of callus volume in the treated animals was seen. 28 days after trauma the increase of callus volume in the treatment group was significantly higher in comparison to the control group. Osteonecrosis was not seen.



Locally applied bisphosphonates increase the callus volume in fracture healing.
Department of Trauma Surgery, University Medical Center of Schleswig-Holstein, Campus Kiel, Kiel, Germany. leif.menzdorf@uksh.de.
Leif
L
Menzdorf
Department of Trauma Surgery, University Medical Center of Schleswig-Holstein, Campus Kiel, Kiel, Germany.
Matthias
M
Weuster
Department of Trauma Surgery, University Medical Center of Schleswig-Holstein, Campus Kiel, Kiel, Germany.
Tim
T
Klüter
Department of Trauma Surgery, University Medical Center of Schleswig-Holstein, Campus Kiel, Kiel, Germany.
Stefan
S
Brüggemann
Department of Trauma Surgery, University Medical Center of Schleswig-Holstein, Campus Kiel, Kiel, Germany.
Peter
P
Behrendt
Department of Trauma Surgery, University Medical Center of Schleswig-Holstein, Campus Kiel, Kiel, Germany.
Stefanie
S
Fitchen-Oestern
Department of Trauma Surgery, University Medical Center of Schleswig-Holstein, Campus Kiel, Kiel, Germany.
Deike
D
Varoga
Department of Trauma Surgery, University Medical Center of Schleswig-Holstein, Campus Kiel, Kiel, Germany.
Andreas
A
Seekamp
Department of Oral and Maxillofacial Surgery, University Medical Center of Schleswig-Holstein, Campus Kiel, Kiel, Germany.
Nicolai
N
Purcz
Section of Biomedical Imaging, Department of Radiology and Neuroradiology, University Medical Center of Schleswig-Holstein, Campus Kiel, Kiel, Germany.
Claus C
CC
Glueer
Department of Anatomy and Cell Biology, RWTH Aachen University, Aachen, Germany.
Thomas
T
Pufe
Department of Trauma Surgery, University Medical Center of Schleswig-Holstein, Campus Kiel, Kiel, Germany.
Sebastian
S
Lippross
D000818:Animals; D050071:Bone Density Conservation Agents / Q000008:administration & dosage* / Q000009:adverse effects / Q000627:therapeutic use*; D003094:Collagen / Q000737:chemistry; D004164:Diphosphonates / Q000008:administration & dosage* / Q000009:adverse effects / Q000627:therapeutic use*; D004195:Disease Models, Animal; D005260:Female; D005264:Femoral Fractures / Q000188:drug therapy* / Q000601:surgery; D005269:Femur / Q000187:drug effects / Q000502:physiology* / Q000601:surgery; D005594:Fracture Fixation, Intramedullary; D017102:Fracture Healing / Q000187:drug effects*; D006801:Humans; D000077268:Pamidronate; D051381:Rats; D017208:Rats, Wistar; D054457:Tissue Scaffolds / Q000737:chemistry; D055114:X-Ray Microtomography
D050071:Bone Density Conservation Agents; D004164:Diphosphonates; D003094:Collagen; D000077268:Pamidronate