Graphene oxide-based electrochemical label-free detection of glycoproteins down to aM level using a lectin biosensor.
A label-free ultrasensitive impedimetric biosensor with lectin immobilised on graphene oxide (GO) for the detection of glycoproteins from 1 aM is shown here. This is the first time a functional lectin biosensor with lectin directly immobilised on a graphene-based interface without any polymer modifier has been described. The study also shows that hydrophilic oxidative debris present on GO has a beneficial effect on the sensitivity of (8.46 ± 0.20)% per decade for the lectin biosensor compared to the sensitivity of (4.52 ± 0.23)% per decade for the lectin biosensor built up from GO with the oxidative debris washed out.
Department of Glycobiotechnology, Institute of Chemistry, Slovak Academy of Sciences, Dubravska cesta 9, 845 38 Bratislava, Slovakia. Jan.Tkac@savba.sk.
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Klukova
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Filip
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Belicky
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Vikartovska
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Tkac
D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D006023:Glycoproteins / Q000032:analysis*; D006108:Graphite; D037102:Lectins / Q000737:chemistry*; D010087:Oxides
D006023:Glycoproteins; D037102:Lectins; D010087:Oxides; D006108:Graphite