Expression of the Mir-133 and Bcl-2 could be affected by swimming training in the heart of ovariectomized rats.
OBJECTIVE
The beneficial and more potent role of exercise to prevent heart apoptosis in ovariectomized rats has been known. The aim of this study was to examine the effects of swimming training on cardiac expression of Bcl-2, and Mir-133 levels and glycogen changes in the myocyte.


METHODS
Forty animals were separated into four groups as control, sham, ovariectomy (OVX) and ovariectomized group with 8 weeks swimming training (OVX.E). Training effects were evaluated by measuring lipid profiles, Bcl-2 and Mir-133 expression levels in the cardiac tissue. Grafts were analyzed by reverse transcription-polymerase chain reaction for Bcl-2 mRNA and Mir-133 and by Western blot for Bcl-2 protein.


RESULTS
Ovariectomy down-regulated Bcl-2 and Mir-133 expression levels in the cardiac tissue, and swimming training up-regulated their expression significantly (P<0.05).


CONCLUSIONS
Our results showed that regular exercise as a physical replacement therapy could prevent and improve the effects of estrogen deficiency in the cardia.
Department of Physiology, Tabriz University of Medical Sciences, Tabriz, Iran.
Parisa
P
Habibi
Department of Histology & Embryology, Tabriz University of Medical Sciences, Tabriz, Iran.
Alireza
A
Alihemmati
Department of Physiology, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
Alireza
A
NourAzar
Department of Physiology, Tabriz University of Medical Sciences, Tabriz, Iran.
Hadi
H
Yousefi
Tabriz University of Medical Sciences, Tabriz, Iran.
Safieh
S
Mortazavi
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Nasser
N
Ahmadiasl
Bcl-2; Heart; Mir-133; Ovariectomy; Swimming training