Biogenic synthesis and spatial distribution of silver nanoparticles in the legume mungbean plant (Vigna radiata L.).
The present investigation aimed to study the in vivo synthesis of silver nanoparticles (AgNPs) in the legume Vigna radiata. The level of plant metabolites such as total phenolics, lipid, terpenoids, alkaloids and amino acid increased by 65%, 133%, 19%, 67% and 35%, respectively, in AgNO3(100 mg L-1) treated plants compared to control. Whereas protein and sugar contents in the treated plants were reduced by 38% and 27%, respectively. FTIR analysis of AgNO3(20-100 mg L-1) treated plants exhibited changes in the IR regions between 3297 and 3363 cm-1, 1635-1619 cm-1, 1249-1266 cm-1and that corresponded to alterations in OH groups of carbohydrates, OH and NH groups of amide I and II regions of protein, when compared with the control. Transmission electron micrographs showed the spatial distribution of AgNPs in the chloroplast, cytoplasmic spaces, vacuolar and nucleolar plant regions. Metal quantification in different tissues of plants exposed to 20-100 mg L-1AgNO3showed about a 22 fold accumulation of Ag in roots as compared to shoots. The phytotoxic parameters such as percent seed germination and shoot elongation remained almost unaltered at low AgNO3doses (20-50 mg L-1). However, at higher levels of exposure (100 mg L-1), the percent seed germination as well as root and shoot elongation exhibited concentration dependent decline. In conclusion, synthesis of AgNPs in V. radiata particularly at lower doses of AgNO3, could be used as a sustainable and environmentally safe technology for large scale production of metal nanoparticles.
Department of Environmental Science, Babasaheb Bhimrao Ambedkar University, Lucknow, 226025, India.
Rima
R
Kumari
Department of Environmental Microbiology, Babasaheb Bhimrao Ambedkar University, Lucknow, 226025, India.
Jay Shankar
JS
Singh
Department of Environmental Science, Babasaheb Bhimrao Ambedkar University, Lucknow, 226025, India. Electronic address: dpsingh_lko@yahoo.com.
Devendra Pratap
DP
Singh
D000470:Alkaloids / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis; D055442:Metabolome / Q000187:drug effects; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000737:chemistry* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D036103:Nanotechnology / Q000379:methods / Q000639:trends; D010636:Phenols / Q000378:metabolism; D064209:Phytochemicals / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D018520:Plant Shoots / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D036226:Seedlings / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D012834:Silver / Q000737:chemistry* / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared; D013729:Terpenes / Q000378:metabolism; D000070658:Vigna / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism
D000470:Alkaloids; D000596:Amino Acids; D008055:Lipids; D010636:Phenols; D064209:Phytochemicals; D013729:Terpenes; D012834:Silver
Phytotoxicity; Plant metabolites; Silver nanoparticles; Spatial metal distribution; Vigna radiata