Microclimatic variation in UV perception and related disparity in tropane and quinolizidine alkaloid composition of Atropa acuminata, Lupinus polyphyllus and Hyoscyamus niger.
The aim of current research was to evaluate the physiological adjustment in three medicinal herbs viz., Atropa acuminata, Lupinus polyphyllus and Hyoscyamus niger to the winter period characterised by intense UV flux in Kashmir valley across the North Western Himalaya. Quinolizidine (QA) and tropane alkaloid (TA) concentrations were analysed in these herbs thriving at two different altitudes via GC-MS and correlated by PCA analysis. This study investigated the hypothesis that UV reflectance and absorbance at low temperatures are directly related to disparity in alkaloid accumulation. Among QAs in L. polyphyllus, ammodendrine and lupanine accumulated at higher concentration and exhibited significant variation of 186.36% and 95.91% in ammodendrine and lupanine respectively in both sites. Tetrahydrohombifoline displayed non-significant variation of about 9.60% irrespective of sites. Among tropane alkaloid (TA), hyoscyamine was recorded as the most abundant constituent irrespective of the plant and site while apotropine accumulated in lesser quantity in A. acuminata than H. niger. However, apotropine demonstrated significant variation of 175% among both sites. The final concentration of quinolizidine (QA) and tropane alkaloid (TA) reflects the interplay between reflectance and absorbance of UV radiation response field. These findings suggest that spectral response of UV light contributes directly to alkaloid biosynthesis.
Centre of Research for Development, University of Kashmir, Srinagar 190006, India.
Sumira
S
Jan
Centre of Research for Development, University of Kashmir, Srinagar 190006, India.
Azra N
AN
Kamili
Centre of Research for Development, University of Kashmir, Srinagar 190006, India.
Javid A
JA
Parray
Plant Biotechnology Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu 180 001, India.
Yashbir S
YS
Bedi
Department of Botany and Microbiology, College of Sciences, King Saud University, Riyadh, Saudi Arabia; Department of Botany, S.P. College, Srinagar 190001, Jammu and Kashmir, India. Electronic address: parvaizbot@yahoo.com.
Parvaiz
P
Ahmad
D000470:Alkaloids / Q000032:analysis* / Q000096:biosynthesis; D032282:Atropa / Q000737:chemistry* / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D032304:Hyoscyamus / Q000737:chemistry* / Q000378:metabolism; D031297:Lupinus / Q000737:chemistry* / Q000378:metabolism; D010880:Piperidines / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D025341:Principal Component Analysis; D011725:Pyridines / Q000032:analysis; D054837:Quinolizidines / Q000737:chemistry; D013034:Sparteine / Q000031:analogs & derivatives / Q000032:analysis; D013696:Temperature; D014326:Tropanes / Q000737:chemistry; D014466:Ultraviolet Rays
D000470:Alkaloids; D010880:Piperidines; D010936:Plant Extracts; D011725:Pyridines; D054837:Quinolizidines; D014326:Tropanes; C008298:lupanine; D013034:Sparteine; C068578:ammodendrine
Environmental factors; GC–MS; Low temperature; Quinolizidine; Tropane alkaloids