Simple high-performance liquid chromatography method for formaldehyde determination in human tissue through derivatization with 2,4-dinitrophenylhydrazine.
A simple high-performance liquid chromatography method has been developed for the determination of formaldehyde in human tissue. FA Formaldehyde was derivatized with 2,4-dinitrophenylhydrazine. It was extracted from human tissue with ethyl acetate by liquid-liquid extraction and analyzed by high-performance liquid chromatography. The calibration curve was linear in the concentration range of 5.0-200 μg/mL. Intra- and interday precision values for formaldehyde in tissue were <6.9%, and accuracy (relative error) was better than 6.5%. The extraction recoveries of formaldehyde from human tissue were between 88 and 98%. The limits of detection and quantification of formaldehyde were 1.5 and 5.0 μg/mL, respectively. Also, this assay was applied to liver samples taken from a biopsy material.
Department of Analytical Chemistry, Faculty of Pharmacy, Ataturk University, Erzurum, Turkey.
Bilal
B
Yilmaz
Department of Toxicology, Faculty of Pharmacy, Ataturk University, Erzurum, Turkey.
Ali
A
Asci
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Ataturk University, Erzurum, Turkey.
Kaan
K
Kucukoglu
Erzurum High Vocational School of Health, Ataturk University, Erzurum, Turkey.
Mevlut
M
Albayrak
D002851:Chromatography, High Pressure Liquid; D005557:Formaldehyde / Q000032:analysis*; D006801:Humans; D008099:Liver / Q000737:chemistry*; D015394:Molecular Structure; D010659:Phenylhydrazines / Q000032:analysis*
D010659:Phenylhydrazines; D005557:Formaldehyde; C004787:2,4-dinitrophenylhydrazine
Derivatization; Formaldehyde; Liquid-liquid extraction; Liver tissue