[Effect of mouse nerve growth factor on cognitive impairment in whole brain irradiation rats].
OBJECTIVE
To investigate the positive effects of mouse nerve growth factor(mNGF) on cognitive impairment in whole brain irradiation rats.


METHODS
Fifty-five male Sprague-Dawley rats were randomly divided into normal control group, mNGF treated group, saline treated group.mNGF treated group and saline treated group were cranial irradiated at a single dose of 12 Gy by X-ray.30 days after radiation each group were treated with correspondent drugs.60 days after radiation, Morris water maze experiment, EB leakage of the brain, and expressions of neuN, vWF, ZO-1 in hippocampus by immunofluorescence, and expressions of neuN, vWF, ZO-1, VEGF and GFAP in hippocampus by Western blot were tested and analyzed.


RESULTS
The escape latencies: normal control group< mNGF treated group< saline treated group; the numbers of crossing hidden platform in these 3 groups were 3.00± 1.08, 1.50± 1.08, 0.38± 0.48 times (P<0.01). EB leakage of these 3 groups were 0.14±0.14, 0.66±0.20 and 1.36±0.27 μg/g (P<0.05). In immunofluorescence, expressions of neuN, vWF and ZO-1: normal control group> mNGF treated group> saline treated group.In Western blot, expressions of neuN, vWF and ZO-1: normal control group> mNGF treated group> saline treated group, yet the expressions of VEGF and GFAP: normal control group< mNGF treated group< saline treated group.


CONCLUSIONS
mNGF ameliorates cognitive impairment after whole brain irradiation.
Department of Neurology, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China.
G Y
GY
He
H W
HW
Huang
Z Z
ZZ
Deng
J J
JJ
Guo
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects; D003071:Cognition / Q000187:drug effects*; D060825:Cognitive Dysfunction / Q000188:drug therapy*; D006624:Hippocampus / Q000378:metabolism; D008297:Male; D051379:Mice; D020932:Nerve Growth Factor / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014965:X-Rays
D020932:Nerve Growth Factor