[Expressions and Clinical Significance of Autophagy-related Genes ATG2B, ATG4D, ATG9B in Breast Carcinoma].
OBJECTIVE
To investigate the expressions and clinical significance of autophagy-related gene 2B (ATG2B), autophagy-related gene 4D (ATG4D), autophagy-related gene 9B (ATG9B) in breast cancer cell lines and breast cancer.


METHODS
Cancer Browser screening was applied to study the differential expressions of ATG2B, ATG4D, ATG9B genes in breast cancer. Quantitative Real-time PCR was used to measure the expressions of these three genes in human breast cancer cell lines (MCF-7, MDA-MB-231, MDA-MB-435S and ZR-75-30) as well as 83 cases of breast cancer samples with paired normal breast tissues. The relationships between the expressions of these genes and clinicopathological characteristics were further analyzed.


RESULTS
Cancer Browser screening found abnormal expressions of ATG2B, ATG4D, ATG9B in breast cancer (P = 0.015, P = 0.028, P = 0.040). All four breast cancer cell lines expressed ATG2B, ATG4D, ATG9B, and the expression of ATG2B, ATG9B was significantly lower than positive control (P < 0.001), but ATG4D expression was higher than positive control (P < 0.001). The expressions of ATG2B, ATG4D, ATG9B in breast cancer were all lower than those in the adjacent normal tissues (P < 0.001, P = 0.031, P < 0.001). Furthermore, the expression of ATG2B was correlated with ER (r = 0.949, P = 0.015), and the expression of ATG4D was related to age (r = -0.449, P = 0.005), the expression of ATG9B was correlated to lymph node metastasis and cytokeratin 5/6 status (r = 0.339, P = 0.043; r = 0.533, P = 0.043).


CONCLUSIONS
ATG2B, ATG4D, ATG9B genes shows low expressions in breast cancer, which may become new molecular markers for the prognosis of breast cancer.
Xue-mei
XM
Zhang
Hong-jiang
HJ
Li
Da
D
Wang
Chuan
C
Li
Qu
Q
Chen
Chang-long
CL
Li
D001343:Autophagy; D000071183:Autophagy-Related Proteins; D001943:Breast Neoplasms / Q000235:genetics*; D045744:Cell Line, Tumor; D003546:Cysteine Endopeptidases / Q000235:genetics*; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis; D008565:Membrane Proteins / Q000235:genetics*; D011379:Prognosis; D033921:Vesicular Transport Proteins / Q000235:genetics*
C572313:ATG2B protein, human; C503283:ATG9B protein, human; D000071183:Autophagy-Related Proteins; D008565:Membrane Proteins; D033921:Vesicular Transport Proteins; C543490:ATG4D protein, human; D003546:Cysteine Endopeptidases