Elucidating the physiological and biochemical responses of different tobacco (Nicotiana tabacum) genotypes to lead toxicity.
In the present study, the effects of lead (Pb) uptake and toxicity were investigated in a hydroponic culture using 7 tobacco (Nicotiana tabacum L.) genotypes (Bina 1 [B1], Kutsaga Mammoth 10 [KM10], Nanjing 3 [N3], Kutsaga 35 [K35], Kutsaga E1 [KE1], Cocker 176 [C176], and Kutsaga RK6 [KRK6]) that differed in Pb tolerance. Lead was applied as a solution of Pb nitrate at concentrations of 0 μM, 10 μM, 250 μM, and 500 μM. After 4 wk of Pb treatment, tissue biomass and photosynthetic parameters were measured and elemental analysis was performed. The results showed decreases in growth and photosynthetic parameters with increases in Pb concentration compared with the control. The least reduction in the recorded physiological parameters was noted in K35, whereas the greatest reduction was observed in N3, which is an obvious indication of genotypic differences. Activities of peroxidase, catalase, and malondialdehyde increased significantly with increases in Pb concentration, with genotypes K35 and N3 showing the least and the greatest reduction, respectively. The results demonstrate the phototoxic nature of Pb on plants, and it can be concluded that in Pb-prone areas genotypes K35 and B1 can be used for cultivation because they can grow efficiently in the presence of high Pb concentrations while restricting Pb uptake in the aboveground parts, as seen by the higher Pb tolerance index. Environ Toxicol Chem 2017;36:175-181. © 2016 SETAC.
Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, People's Republic of China.
Antony
A
Maodzeka
Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, People's Republic of China.
Nazim
N
Hussain
Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, People's Republic of China.
Liquan
L
Wei
Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, People's Republic of China.
Gerald
G
Zvobgo
Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, People's Republic of China.
James Mutemachani
JM
Mapodzeke
Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, People's Republic of China.
Muhammad Faheem
MF
Adil
Department of Development Studies, Comsats Institute of Information Technology, Abbottabad, 22060, Pakistan.
Salma
S
Jabeen
Guizhou Provincial Tobacco Company, Qianxinan Branch, People's Republic of China.
Feng
F
Wang
Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, People's Republic of China.
Lixi
L
Jiang
Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, People's Republic of China.
Imran Haider
IH
Shamsi
D000975:Antioxidants / Q000378:metabolism*; D018533:Biomass; D002374:Catalase / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D005838:Genotype; D018527:Hydroponics; D007854:Lead / Q000378:metabolism / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D009566:Nitrates; D010788:Photosynthesis / Q000187:drug effects* / Q000235:genetics; D018515:Plant Leaves / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism / Q000502:physiology; D012989:Soil Pollutants / Q000378:metabolism / Q000633:toxicity*; D014026:Nicotiana / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism / Q000502:physiology
D000975:Antioxidants; D009566:Nitrates; D012989:Soil Pollutants; D002734:Chlorophyll; D007854:Lead; D008315:Malondialdehyde; D002374:Catalase
Genotypic response; Lead toxicity; Photosynthesis; Physiological mechanism; Tobacco