A versatile two-photon fluorescent probe for ratiometric imaging E. coliβ-galactosidase in live cells and in vivo.
We have described the design, synthesis, spectroscopy and biological applications of NI-βGal, a versatile fluorescent probe to detect E. coliβ-galactosidase in live cells and mice sensitively and directly, which holds great promise for its application in biomedical research such as gene therapy for cancer in the future.
State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics (DICP), Chinese Academy of Sciences (CAS), Dalian 116023, P. R. China. klhan@dicp.ac.cn.
Xue-Xiang
XX
Zhang
Hao
H
Wu
Peng
P
Li
Zong-Jin
ZJ
Qu
Ming-Qian
MQ
Tan
Ke-Li
KL
Han
D015374:Biosensing Techniques; D002470:Cell Survival; D004926:Escherichia coli / Q000201:enzymology*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D005696:Galactosidases / Q000032:analysis* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D057054:Molecular Imaging; D017785:Photons
D005456:Fluorescent Dyes; D005696:Galactosidases