Cholinergic enzymes and inflammatory markers in rats infected by Sporothrix schenckii.
The aim of this study was to evaluate the cholinesterase activity in serum, whole blood, and lymphocytes, as well as to verify its relation to immune response in rats experimentally infected by Sporothrix schenckii. For this study, 63 Wistar rats (Rattus norvegicus), male, adult were divided into three groups: the negative control group (GC: n = 21), the group infected subcutaneously (GSC: n = 21), and the group infected intraperitoneally (GIP: n = 21). The groups were divided into subgroups and the following variables were evaluated at 15, 30, and 40 days post-infection (PI): acetylcholinesterase (AChE) activity in lymphocytes and whole blood, butyrylcholinesterase (BChE) activity in serum, cytokines levels (IL-1, IL-6, TNFα, and INF-γ), immunoglobulins levels (IgA, IgG, IgM, and IgE), and protein profile by electrophoresis. Both infected groups showed increased levels of inflammatory parameters (P < 0.05) in tissue and inflammatory infiltrates. The activities of AChE in lymphocytes and BChE in serum increased (P < 0.05) significantly in animals from the GSC group on day 40 PI compared to the GC group. Regarding the GIP, there was a marked increase in the AChE activity in lymphocytes on days 30 and 40 PI, and in whole blood on days 15, 30, and 40 PI compared to GC. Furthermore, IL-10, an anti-inflammatory cytokine, was also present in high levels during chronic systemic S. schenckii infections in animals. Therefore, it is concluded that cholinesterase has an important modulatory role in the immune response during granulomatous infection by S. schenckii.
Department of Small Animals, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Veronica S P
VS
Castro
Department of Animal Science, Universidade do Estado de Santa Catarina, Chapecó, SC, Brazil.
Aleksandro S
AS
Da Silva
Department of Small Animals, Universidade de Passo Fundo, Passo Fundo, RS, Brazil.
Márcio M
MM
Costa
Department of Small Animals, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Francine C
FC
Paim
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
Sydney H
SH
Alves
Department of Small Animals, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Sonia T A
ST
Lopes
Department of Small Animals, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Cássia B
CB
Silva
Department of Small Animals, Universidade de Cruz Alta, Cruz Alta, RS, Brazil.
Patrícia
P
Wolkmer
Departament of Small Animals, Pontifical Catholic University, Curitiba, PR, Brazil.
Jorge Luiz C
JL
Castro
Department of Small Animals, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Bianca S
BS
Cecco
Department of Health Sciences, Universidade Luterana do Brazil, Santa Maria, RS, Brazil.
Marta M M F
MM
Duarte
Department of Biochemical and Molecular Biology, UFSM, Santa Maria, RS, Brazil.
Maria Rosa C
MR
Schetinger
Department of Small Animals, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Dominguita L
DL
Graça
Department of Small Animals, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil. Electronic address: cmelazzoandrade1@gmail.com.
Cinthia M
CM
Andrade
D000818:Animals; D000908:Antibodies, Fungal / Q000097:blood; D002802:Cholinesterases / Q000032:analysis*; D016207:Cytokines / Q000032:analysis; D004195:Disease Models, Animal; D007249:Inflammation / Q000473:pathology*; D008214:Lymphocytes / Q000201:enzymology; D008297:Male; D011506:Proteins / Q000032:analysis; D017208:Rats, Wistar; D044967:Serum / Q000201:enzymology; D013175:Sporothrix / Q000254:growth & development*; D013174:Sporotrichosis / Q000473:pathology*
D000908:Antibodies, Fungal; D016207:Cytokines; D011506:Proteins; D002802:Cholinesterases
Acetylcholinesterase; Cytokines; Inflammatory markers; Sporotrichosis