Transparent, resilient human amniotic membrane laminates for corneal transplantation.
This study evaluated a new technique to toughen and optically clarify human amniotic membrane (AM) tissue, which is naturally thin and clouded, and determined the suitability of the altered tissue for corneal transplantation. The technique created a tissue laminate by repeatedly depositing wet layers of AM and dehydrating them, followed by chemical cross-linking to tighten integration at the layer interfaces and within the layers, thereby improving the physical properties of the laminates by increasing light transmittance and mechanical strength. Interestingly, this improvement only occurred in laminates with at least 4 layers. Cross-linking also improved the resistance of the laminates to collagenase degradation, such as occurs in corneal melting. This study also confirmed that the AM tissue was biocompatible by inserting AM monolayers into the corneal stroma of rabbits, and by performing lamellar keratoplasty in rabbits with cross-linked AM laminates. The laminates were sufficiently thick and resilient to need only one set of sutures, whereas in previously described multi-layer AM transplantation technique, each layer required separate sutures. The current findings are a promising advance in the engineering of novel biomaterials and the alteration of existing tissues for medical use.
Department of Ophthalmology and Visual Science, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan(1).
Takehiro
T
Hariya
RIKEN Advanced Center for Computing and Communication, Preventive Medicine and Applied Genomics Unit, Yokohama, Kanagawa, Japan(2); RIKEN Center for Life Science Technologies, Division of Genomic Technologies, Nucleic Acid Diagnostic System Development Unit, Yokohama, Kanagawa, Japan(3). Electronic address: yuji.tanaka@riken.jp.
Yuji
Y
Tanaka
Department of Ophthalmology and Visual Science, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan(1). Electronic address: yokokura@oph.med.tohoku.ac.jp.
Shunji
S
Yokokura
Department of Ophthalmology and Visual Science, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan(1).
Toru
T
Nakazawa
D000650:Amnion / Q000737:chemistry* / Q000637:transplantation*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000627:therapeutic use; D001696:Biomechanical Phenomena; D003315:Cornea / Q000601:surgery; D003319:Corneal Stroma / Q000601:surgery*; D016039:Corneal Transplantation; D003432:Cross-Linking Reagents / Q000737:chemistry; D003890:Desiccation; D006801:Humans; D008297:Male; D011817:Rabbits
D001672:Biocompatible Materials; D003432:Cross-Linking Reagents
Biomimetic material; Cornea; Fibrous tissue; Soft tissue biomechanics; Suture; Transplantation