JC virus/human immunodeficiency virus 1 co-infection in the Brazilian Amazonian region.
JC virus (JCV) is a member of the Polyomaviridae family and is associated to a severe disease known as progressive multifocal leukoencephalopathy, PML, which is gradually increasing in incidence as an opportunistic infection among AIDS patients. The present study aimed to investigate the occurrence of JCV among HIV-1 carriers including their types and molecular subtypes and the possible association with disease. Urine samples from 66 HIV-1 infected subjects were investigated for the presence of the virus by amplifying VP1 (215bp) and IG (610bp) regions using the polymerase chain reaction. JCV was detected in 32% of the samples. The results confirmed the occurrence of type B (subtype Af2); in addition, another polyomavirus, BKV, was also detected in 1.5% of samples of the HIV-1 infected subjects. Apparently, there was no significant difference between mono- (HIV-1 only) and co-infected (HIV-1/JCV) subjects regarding their TCD4(+)/TCD8(+) lymphocyte counts or HIV-1 plasma viral load. Self admitted seizures, hearing and visual loses were not significantly different between the two groups.
Universidade Federal do Pará (UFPA), Instituto de Ciências Biológicas, Laboratório de Vírus, Belem, Para, Brazil. Electronic address: ivallinoto@ufpa.br.
Izaura Maria Vieira
IM
Cayres-Vallinoto
Universidade Federal do Pará (UFPA), Instituto de Ciências Biológicas, Laboratório de Vírus, Belem, Para, Brazil.
Antonio Carlos Rosário
AC
Vallinoto
Universidade Federal do Pará (UFPA), Instituto de Ciências Biológicas, Laboratório de Vírus, Belem, Para, Brazil.
Giselle Priscila Dos Anjos
GP
Pena
Universidade Federal do Pará (UFPA), Instituto de Ciências Biológicas, Laboratório de Vírus, Belem, Para, Brazil.
Vânia Nakauth
VN
Azevedo
Universidade Federal do Pará (UFPA), Instituto de Ciências Biológicas, Laboratório de Vírus, Belem, Para, Brazil.
Luiz Fernando Almeida
LF
Machado
Universidade Federal do Pará (UFPA), Instituto de Ciências Biológicas, Laboratório de Vírus, Belem, Para, Brazil.
Marluísa de Oliveira Guimarães
Mde O
Ishak
Universidade Federal do Pará (UFPA), Instituto de Ciências Biológicas, Laboratório de Vírus, Belem, Para, Brazil.
Ricardo
R
Ishak
D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis / Q000652:urine / Q000821:virology*; D000293:Adolescent; D000328:Adult; D001938:Brazil; D018791:CD4 Lymphocyte Count; D002648:Child; D060085:Coinfection / Q000821:virology; D003430:Cross-Sectional Studies; D004279:DNA, Viral / Q000652:urine; D005260:Female; D006801:Humans; D007577:JC Virus / Q000235:genetics* / Q000302:isolation & purification; D007968:Leukoencephalopathy, Progressive Multifocal / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D019562:Viral Load; D055815:Young Adult
D004279:DNA, Viral
Brazilian Amazon region; Co-infection; HIV-1; JCV