Algoriphagus roseus sp. nov., isolated from a hexachlorocyclohexane-contaminated dumpsite.
A Gram-staining negative, reddish-pink, non-motile, rod-shaped bacterial strain designated W29T, was isolated from a hexachlorocyclohexane-contaminated dumpsite located in the northern part of India at Ummari Village, Lucknow. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain W29T formed a lineage within the genus Algoriphagusand exhibited highest sequence similarity to Algoriphagus trabzonensis MS7T (98.8 %), followed by Algoriphagusalkaliphilus AC-74T (97.1 %). The 16S rRNA gene sequence similarity between strain W29T and other species of the genus Algoriphagusranged from 93.3-98.8 %. The DNA-DNA relatedness between strain W29T and A. trabzonensisMS7T was 47 % and with other related strains was found to be less than 45 %, confirming strain W29T represents a novel species. The DNA G+C content of strain W29T was 46.2 mol%. Strain W29T was oxidase- and catalase-positive. The major fatty acids (>10 %) of strain W29T were iso-C15 : 0, summed feature 9 (comprising 10-methyl C16 : 0 and/or iso-C17 : 1ω9c) and summed feature 3 (comprising C16 : 1ω6c and/or C16 : 1ω7c). The respiratory quinone was MK-7. The polar lipid profile consisted of phosphatidylethanolamine, diphosphatidylglycerol, two unidentified aminolipids, an unidentified aminophospholipid, an unidentified phospholipid and unidentified lipids. On the basis of the results obtained from DNA-DNA hybridization, and biochemical and physiological tests in this study, strain W29T represents a novel species of the genus Algoriphagus for which the name Algoriphagus roseus sp. nov. is proposed. The type strain is W29T (=KCTC 42940T=MCC 2876T=DSM 100160T).
Molecular Biology Laboratory, Department of Zoology, University of Delhi, 110007 Delhi, India.
Puneet
P
Kohli
Molecular Biology Laboratory, Department of Zoology, University of Delhi, 110007 Delhi, India.
Namita
N
Nayyar
Molecular Biology Laboratory, Department of Zoology, University of Delhi, 110007 Delhi, India.
Anukriti
A
Sharma
Molecular Biology Laboratory, Department of Zoology, University of Delhi, 110007 Delhi, India.
Amit Kumar
AK
Singh
Molecular Biology Laboratory, Department of Zoology, University of Delhi, 110007 Delhi, India.
Rup
R
Lal
D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D001556:Hexachlorocyclohexane / Q000032:analysis; D007194:India; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012989:Soil Pollutants / Q000032:analysis*; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D024482:Vitamin K 2; D001556:Hexachlorocyclohexane; C062629:menaquinone 7