Cissus quadrangularis prevented the ovariectomy induced oxidative stress in the femur of adult albino rats.
The increasing evidence suggesting the role of free radicals in bone resorption and bone loss prompted us to explore whether the consumption of antioxidant rich medicinal plant C. quadrangularis modifies antioxidant status in ovariectomized rats.


METHODS
Twenty four female adult rats, 90days old showing regular estrous cycles were used for the present study. The animals were divided into two groups. The Group-1 rats (n=6) were sham operated and Group-II rats were bilaterally ovariectomized (n=18) and treated with C. quadrangularis for sixty days (100mg/kg body weight and 250mg/kg body weight). After sixty days, the rats were killed, femora were dissected out, minced and homogenized in Tris-HCl buffer (pH 7.4) and the supernatant was collected and used for biochemical assays.


RESULTS
Ovariectomy registered a decrease (p<0.05) in the activities of SOD, GPx, GST, ALP, collagen content and increased (p<0.05) the activities of TRAP and lipid peroxidation. Simultaneous administration of C. quadrangularis maintained the enzyme activities in ovariectomized rats.


CONCLUSIONS
C. quadrangularis, a natural herb may be used to treat the estrogen deficiency/menopause onset and ovariectomy induced oxidative stress.
Department of Biochemistry, Karpagam University, Eachanari, Coimbatore, Tamilnadu 641021, India; Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani campus, Chennai, 600113, India. Electronic address: sridhar.m@kahedu.edu.in.
Sridhar
S
Muthusami
Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani campus, Chennai, 600113, India.
Vasudevan
V
Gopalakrishnan
Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani campus, Chennai, 600113, India; Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843, USA.
Jone A
JA
Stanley
Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani campus, Chennai, 600113, India.
Senthilkumar
S
Krishnamoorthy
Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani campus, Chennai, 600113, India.
Ramachandran
R
Ilangovan
Department of Biochemistry, Karpagam University, Eachanari, Coimbatore, Tamilnadu 641021, India.
Velliyur K
VK
Gopalakrishnan
Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani campus, Chennai, 600113, India; Faculty of Allied Health Sciences, Chettinad University, Kelambakkam, Chennai 603103, India. Electronic address: n_srini2000@yahoo.com.
Narasimhan
N
Srinivasan
D000375:Aging; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D032422:Cissus / Q000737:chemistry*; D003094:Collagen / Q000378:metabolism; D005260:Female; D005269:Femur / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology*; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D010052:Ovariectomy / Q000009:adverse effects*; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D000071681:Tartrate-Resistant Acid Phosphatase / Q000378:metabolism
D010936:Plant Extracts; D003094:Collagen; D006861:Hydrogen Peroxide; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D000469:Alkaline Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase
Bone loss; C. quadrangularis; Ovariectomy; Oxidative stress