Prevalence of beta lactamase producing species of pseudomonas and acinetobacter in pediatric burn patients.
Burn wound infection is a major cause of morbidity and mortality in burn victims. Pseudomonas and Acinetobacter species are among the most common organisms complicating burn wounds. Presence of extended spectrum ß-lactamase (ESBL) and metallo-ß-lactamase (MBL) genes plays an important role in spreading ß-lactam resistant strains of these organisms and is a serious condition in the treatment of the affected patients. As a result, we aimed to determine the prevalence of SHV, TEM, PER and VIM ß-lactamases in Pseudomonas and Acinetobacter species isolates from burn wound swabs of children with burn injury. In this descriptive observational study, 107 Pseudomonas and Acinetobacter isolates collected from burn patients were subjected to PCR assay. Using PCR method and DNA sequencing, the existence of SHV-, TEM-, PER- and VIM-type ß-lactamase encoding genes were determined. Out of the 107 Pseudomonas and Acinetobacter isolates, 66 (77.6%) were ESBL positive, 26.2% were positive for SHV gene, 37.4% were positive for TEM gene, 14% were positive for PER gene and 15.9% of them harbored VIM gene. More than half of the Pseudomonas and Acinetobacter strains in our pediatric burn unit harbor ß-lactamase encoding genes that make them resistant to a wide range of ß-lactam antibiotics. Consequently, it is suggested to choose an appropriate antibiotic regimen based on the antibiogram pattern of the strains.
Shahid Motahari Hospital, Iran University of Medical Sciences, Tehran, Iran.
B
B
Sobouti
Ali-Asghar Children's Hospital, Iran University of Medical Sciences, Tehran, Iran.
N
N
Khosravi
Iran University of Medical Sciences, Tehran, Iran.
A
A
Daneshvar
Shahid Beheshti University of Medical Sciences, Tehran, Iran.
S
S
Fallah
Iran University of Medical Sciences, Tehran, Iran.
M
M
Moradi
Ali-Asghar Children's Hospital, Iran University of Medical Sciences, Tehran, Iran.
Y
Y
Ghavami
Acinetobacter species; Pseudomonas species; burn wound; extended-spectrum ß-lactamases; metallo-ß-lactamase