FRET Imaging of Enzymatic Activities Using Smart Probes.
Tumor-related enzymes are extensively involved in the occurrence, development, invasion, and metastasis of tumors, indicating they hold the potential to serve as biomarkers for tumor diagnosis and treatment. Smart probes based on characteristic activities of these enzymes and fluorescence resonance energy transfer (FRET) have been widely developed for fluorescent imaging of enzymatic activities. Here, we describe the detailed chemical strategies for construction of smart probe and its application for FRET imaging of fibroblast activation protein alpha in vitro and in vivo.
School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, P. R. China.
Jinbo
J
Li
School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, P. R. China.
Yan
Y
Zhang
Molecular Imaging Program at Stanford (MIPS), Department of Radiology and Bio-X Program, Stanford University, Lucas Center, P095, Mail Code 5484, 1201 Welch Road, Stanford, CA, 94305, USA. zcheng@stanford.edu.
Zhen
Z
Cheng
D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis; D045744:Cell Line, Tumor; D004798:Enzymes / Q000032:analysis*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D051379:Mice; D009369:Neoplasms / Q000201:enzymology*; D051381:Rats
D014408:Biomarkers, Tumor; D004798:Enzymes; D005456:Fluorescent Dyes
Enzyme; Fluorescence resonance energy transfer; Fluorescent imaging; Peptide; Smart probe