Separation and nanoencapsulation of antitumor peptides from Chinese three-striped box turtle (Cuora trifasciata).
Chinese three-striped box turtle (Cuora trifasciata), as a freshwater turtle, is used as a tonic food. The purpose of this study was to isolate peptides with cancer growth inhibition activity from trypsin-digested hydrolysates of turtle proteins. The results demonstrated that two fractions T1 and T2 exhibited good inhibition on HepG-2 and MCF-7 cancer cells, with an inhibition of 70.65-89.1%, at 500 μg/mL. Subsequently, three peptides were identified from T1 and T2, including RGVKGPR (T1-1), KLGPKGPR (T1-2), and SSPGPPVH (T2-1). By database search, T2-1 was a completely new peptide; its inhibition activity on MCF-7 cancer cells was the best, up to 70.02% at 500 μg/mL. Then, T1 and T2-1 were nanoencapsulated by chitosan. After nanoencapsulation, the inhibition percentages were 50.23% for the nanoencapsulated T1 on HepG-2 and 46.82% for the encapsulated T2-1 on MCF-7. The release experiment indicated that the encapsulated peptides could be slowly released in simulated gastrointestinal juice.
a College of Food Science and Engineering, South China University of Technology , Guangzhou , China ;
Shengjie
S
He
a College of Food Science and Engineering, South China University of Technology , Guangzhou , China ;|b Infinitus (China) Company Ltd , Guangzhou , China.
Xinliang
X
Mao
b Infinitus (China) Company Ltd , Guangzhou , China.
Ting
T
Zhang
b Infinitus (China) Company Ltd , Guangzhou , China.
Xiaolei
X
Guo
b Infinitus (China) Company Ltd , Guangzhou , China.
Yazhong
Y
Ge
b Infinitus (China) Company Ltd , Guangzhou , China.
Chungwah
C
Ma
a College of Food Science and Engineering, South China University of Technology , Guangzhou , China ;
Xuewu
X
Zhang
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000493:pharmacokinetics / Q000494:pharmacology; D048271:Chitosan / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D061986:MCF-7 Cells; D053769:Nanocapsules / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000493:pharmacokinetics / Q000494:pharmacology; D014426:Turtles
D000970:Antineoplastic Agents; D053769:Nanocapsules; D010455:Peptides; D048271:Chitosan
Cuora trifasciata; antitumor; hydrolysis; nanoencapsulation; peptides