Analysis of Neural Stem Cells from Human Cortical Brain Structures In Vitro.
Comparative immunohistochemical analysis of the neocortex from human fetuses showed that neural stem and progenitor cells are present in the brain throughout the gestation period, at least from week 8 through 26. At the same time, neural stem cells from the first and second trimester fetuses differed by the distribution, morphology, growth, and quantity. Immunocytochemical analysis of neural stem cells derived from fetuses at different gestation terms and cultured under different conditions showed their differentiation capacity. Detailed analysis of neural stem cell populations derived from fetuses on gestation weeks 8-9, 18-20, and 26 expressing Lex/SSEA1 was performed.
N. K. Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia.|V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
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MA
Aleksandrova
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia. rimpol@mail.ru.
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RA
Poltavtseva
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
M V
MV
Marei
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
G T
GT
Sukhikh
D002454:Cell Differentiation; D002478:Cells, Cultured; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006801:Humans; D019579:Neocortex / Q000166:cytology*; D064231:Nestin / Q000378:metabolism; D058953:Neural Stem Cells / Q000502:physiology*; D018874:Spheroids, Cellular / Q000166:cytology
D005904:Glial Fibrillary Acidic Protein; C577484:NES protein, human; D064231:Nestin
differentiation; immunocytochemical and immunohistochemical analysis; neocortex; neural stem cells; regenerative medicine