Identifying diagnostic endocrine markers and changes in endometrial gene expressions during pyometra in cats.
Pyometra is a significant reproductive problem in cats. The aims of this study were to evaluate (i) the immunological profile of queens by studying plasma concentrations of metabolites of prostacyclin I2 (6-keto-PGF1α), leukotriene B4 (LTB4) and leukotriene C4 (LTC4); and (ii) the gene transcription profiles of Toll-like receptors (TLRs) 2 and 4 (TLR2/4), PGE2-synthase (PGES), PGF2α-synthase (PGFS) and prostaglandin-endoperoxide synthase 2 (PTGS2) in the feline endometrium throughout the estrous cycle, after medroxyprogesterone acetate (MPA) treatment and during pyometra. The concentration of plasma 6-keto-PGF1α in pyometra was increased in comparison to other groups studied (p<0.01). Endometrial mRNA coding for TLR2 was up-regulated in cats suffering from pyometra compared to other groups (p<0.001). Expression of mRNA for TLR4 was up-regulated in endometria originating from MPA-treated cats, pyometra and late diestrus cats, compared with tissues from cats during estrus and anestrus (p<0.05). As expected, endometrial mRNA for PTGS2 was up-regulated only in pyometra, compared with other groups (p<0.001). Similarly, endometrial mRNA for PGFS was up-regulated in pyometra, compared with endometria from anestrus, late diestrus and from MPA-treated cats (p<0.05), or from cats during estrus (p<0.01). Overall, these results indicate that plasma concentrations of LTB4 and LTC4 are not useful diagnostic markers since they were not increased in queens with pyometra, in contrast to 6-keto-PGF1α. In addition, treatment with MPA evoked neither endocrine nor molecular changes in endometria of cats.
Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10 Str., 10-748 Olsztyn, Poland. Electronic address: e.jursza@pan.olsztyn.pl.
Ewelina
E
Jursza-Piotrowska
Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10 Str., 10-748 Olsztyn, Poland. Electronic address: m.siemieniuch@pan.olsztyn.pl.
Marta J
MJ
Siemieniuch
D015121:6-Ketoprostaglandin F1 alpha / Q000097:blood; D000818:Animals; D002415:Cats; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism*; D030762:Estrous Cycle / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D015289:Leukotrienes / Q000097:blood; D017258:Medroxyprogesterone Acetate / Q000494:pharmacology; D055112:Pyometra / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism / Q000662:veterinary*; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
D015289:Leukotrienes; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D015121:6-Ketoprostaglandin F1 alpha; D017258:Medroxyprogesterone Acetate
Feline endometrium; Leukotrienes; Prostaglandins; Pyometra; Toll-like receptors