Potent Inhibitory Effects of Some Phenolic Acids on Lactoperoxidase.
Lactoperoxidase (LPO) plays a key role in immune response against pathogens. In this study, we examined the effects of some phenolic acids on LPO. For this purpose, bovine milk LPO was purified 380.85-fold with a specific activity of 26.66 EU/mg and overall yield of 73.33% by using Amberlite CG-50 H+resin and CNBr-activated Sepharose-4B-l-tyrosine-sulfanilamide affinity chromatography. After purification, the in vitro effects of phenolic acids (tannic acid, 3,4-dihydroxybenzoic acid, 3,5- dihydroxybenzoic acid, chlorogenic acid, sinapic acid, 4-hydroxybenzoic acid, vanillic acid, salicylic acid, and 3-hydroxybenzoic acid) were investigated on LPO. These phenolic acids showed potent inhibitory effect on LPO. Kivalues for these phenolic acids were found as 0.0129 nM, 0.132 μM, 0.225 μM, 0.286 μM, 0.333 μM, 2.33 μM, 10.82 μM, 0.076 mM, and 0.405 mM, respectively. Sinapic acid and 4-hydroxybenzoic acid exhibited noncompetitive inhibition; 3,4-dihydroxybenzoic acid showed uncompetitive inhibition, and other phenolic acids showed competitive inhibition.
Department of Chemistry, Faculty of Sciences, Istanbul Medeniyet University, Istanbul, 34730, Turkey.
Zeynep
Z
Koksal
Department of Chemistry, Faculty of Science and Arts, Ahi Evran University, Kırşehir, 40000, Turkey.
Zuhal
Z
Alim
Department of Chemistry, Faculty of Sciences, Atatürk University, Erzurum, 25240, Turkey.|Department of Food Science, Faculty of Engineering, Iğdır University, Iğdır, 76000, Turkey.
Sukru
S
Beydemir
Department of Chemistry, Faculty of Sciences, Atatürk University, Erzurum, 25240, Turkey.
Hasan
H
Ozdemir
D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D004791:Enzyme Inhibitors / Q000737:chemistry*; D062385:Hydroxybenzoates / Q000737:chemistry*; D007700:Kinetics; D007784:Lactoperoxidase / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000302:isolation & purification; D008024:Ligands; D008892:Milk / Q000737:chemistry; D008894:Milk Proteins / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000302:isolation & purification; D011485:Protein Binding
D004791:Enzyme Inhibitors; D062385:Hydroxybenzoates; D008024:Ligands; D008894:Milk Proteins; D007784:Lactoperoxidase
Bovine Milk; Inhibition; Lactoperoxidase; Phenolic Acids