Three-dimensional polymer coated 45S5-type bioactive glass scaffolds seeded with human mesenchymal stem cells show bone formation in vivo.
45S5-type bioactive glasses are a promising alternative to established substitutes for the treatment of bone defects. Because the three-dimensional (3D) structure of bone substitutes is crucial for bone ingrowth and formation, we evaluated the osteoinductive properties of different polymer coated 3D-45S5 bioactive glass (BG) scaffolds seeded with human mesenchymal stem cells (hMSC) in vivo. BG scaffolds coated with gelatin, cross-linked gelatin, and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) were seeded with hMSC prior to implantation into severe combined immunodeficiency mice. Newly formed bone was evaluated with histomorphometry and micro-computed tomography. Bone formation was detectable in all groups, whereas the gelatin-coated BG scaffolds showed the best results and should be considered in further studies.
HTRG - Heidelberg Trauma Research Group, Trauma and Reconstructive Surgery, Center of Orthopedics, Traumatology, and Spinal Cord Injury, Heidelberg University Hospital, Schlierbacher Landstraße 200a, 69118, Heidelberg, Germany.
Fabian
F
Westhauser
Clinic of Diagnostic and Interventional Radiology (DIR), Heidelberg University Hospital, Im Neuenheimer Feld 110, 69120, Heidelberg, Germany.
Christian
C
Weis
HTRG - Heidelberg Trauma Research Group, Trauma and Reconstructive Surgery, Center of Orthopedics, Traumatology, and Spinal Cord Injury, Heidelberg University Hospital, Schlierbacher Landstraße 200a, 69118, Heidelberg, Germany.
Matthäus
M
Prokscha
Clinic for Hand-, Plastic- and Reconstructive Surgery, BG-Unfallklinik Ludwigshafen, Ludwig-Guttmann-Straße 13, 67071, Ludwigshafen am Rhein, Germany.
Leonie A
LA
Bittrich
Institute of Biomaterials, University of Erlangen-Nuremberg, Cauerstr. 6, 91058, Erlangen, Germany.
Wei
W
Li
HTRG - Heidelberg Trauma Research Group, Trauma and Reconstructive Surgery, Center of Orthopedics, Traumatology, and Spinal Cord Injury, Heidelberg University Hospital, Schlierbacher Landstraße 200a, 69118, Heidelberg, Germany.
Kai
K
Xiao
Clinic for Hand-, Plastic- and Reconstructive Surgery, BG-Unfallklinik Ludwigshafen, Ludwig-Guttmann-Straße 13, 67071, Ludwigshafen am Rhein, Germany.
Ulrich
U
Kneser
Clinic of Diagnostic and Interventional Radiology (DIR), Heidelberg University Hospital, Im Neuenheimer Feld 110, 69120, Heidelberg, Germany.
Hans-Ulrich
HU
Kauczor
HTRG - Heidelberg Trauma Research Group, Trauma and Reconstructive Surgery, Center of Orthopedics, Traumatology, and Spinal Cord Injury, Heidelberg University Hospital, Schlierbacher Landstraße 200a, 69118, Heidelberg, Germany.
Gerhard
G
Schmidmaier
Institute of Biomaterials, University of Erlangen-Nuremberg, Cauerstr. 6, 91058, Erlangen, Germany.
Aldo R
AR
Boccaccini
HTRG - Heidelberg Trauma Research Group, Trauma and Reconstructive Surgery, Center of Orthopedics, Traumatology, and Spinal Cord Injury, Heidelberg University Hospital, Schlierbacher Landstraße 200a, 69118, Heidelberg, Germany. arash.moghaddam@med.uni-heidelberg.de.
Arash
A
Moghaddam
D000818:Animals; D001846:Bone Development; D001854:Bone Marrow Cells / Q000378:metabolism; D001861:Bone Regeneration; D018786:Bone Substitutes; D002516:Ceramics / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry; D005898:Glass / Q000737:chemistry*; D006801:Humans; D021621:Imaging, Three-Dimensional; D008297:Male; D008422:Materials Testing; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D016513:Mice, SCID; D010012:Osteogenesis; D011108:Polymers / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D055114:X-Ray Microtomography; D055815:Young Adult
D018786:Bone Substitutes; D011108:Polymers; C425330:bioactive glass 45S5; D005780:Gelatin; D017886:Durapatite