A Practical Guide to the HIrisPlex System: Simultaneous Prediction of Eye and Hair Color from DNA.
The HIrisPlex system, which consists of two parts, allows the simultaneous prediction of eye and hair color from DNA, e.g., samples extracted from crime scene evidence. The first part is a highly sensitive multiplex genotyping assay consisting of 24 DNA markers using SNaPshot™ chemistry, for analysis on all Capillary Electrophoresis machines. The second part consists of statistical models that respectively establish eye and hair color prediction probabilities from complete and incomplete genotype profiles using parameters generated from large genotype and phenotype databases. This combined prediction tool constitutes the online system freely available to users. Here we provide a practical guide on how to use the HIrisPlex system for forensic and other DNA applications.
Department of Biology, Indiana University-Purdue University Indianapolis (IUPUI), 723 W. Michigan St., SL 350, Indianapolis, IN, 46202, USA. walshsus@iupui.edu.
Susan
S
Walsh
Department of Forensic Molecular Biology, Erasmus MC University Medical Center Rotterdam, 3000 CB, Rotterdam, The Netherlands.
Manfred
M
Kayser
D004247:DNA / Q000235:genetics*; D005127:Eye Color / Q000235:genetics*; D053803:Forensic Genetics; D006200:Hair Color / Q000235:genetics*; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide; D011237:Predictive Value of Tests; D011336:Probability
D004247:DNA
Eye color; Forensic DNA Phenotyping; HIrisPlex; Hair color; Prediction; SNP