Cytocompatible, Photoreversible, and Self-Healing Hydrogels for Regulating Bone Marrow Stromal Cell Differentiation.
Photo-crosslinking and self-healing have received considerable attention for the design of intelligent materials. A novel photostimulated, self-healing, and cytocompatible hydrogel system is reported. A coumarin methacrylate crosslinker is synthesized to modify the polyacrylamide-based hydrogels. With the [2+2] cyclo-addition of coumarin moieties, the hydrogels exhibit excellent self-healing capacity when they are exposed to light with wavelengths at 280 and 365 nm, respectively. To enhance cell compatibility, a poly (amidoamine) crosslinker is also synthesized. Variations in light exposure times and irradiation wavelengths are found to alter the self-healing property of the hydrogels. The hydrogels are shown to induce a regular cellular pattern. The hydrogels are used to regulate bone marrow stromal cells differentiation. The relative mRNA expressions are recorded to monitor the osteogenic differentiation of the cells.
Department of Biosystem Engineering, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada.
Lianlian
L
Yu
Department of Mechanical Engineering, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada.
Kaige
K
Xu
Department of Mechanical Engineering, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada.
Liangpeng
L
Ge
Department of Mechanical Engineering, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada.
Wenbing
W
Wan
Department of Mechanical Engineering, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada.
Ali
A
Darabi
Department of Mechanical Engineering, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada. malcolm.xing@umanitoba.ca.|Manitoba Institute of Child Health, Winnipeg, MB, R3T 2N2, Canada. malcolm.xing@umanitoba.ca.|Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada. malcolm.xing@umanitoba.ca.
Malcolm
M
Xing
Department of Biosystem Engineering, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada. wen.zhong@umanitoba.ca.
Wen
W
Zhong
D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D003432:Cross-Linking Reagents / Q000737:chemistry; D005786:Gene Expression Regulation / Q000187:drug effects; D020100:Hydrogels / Q000494:pharmacology*; D007091:Image Processing, Computer-Assisted; D008027:Light; D008422:Materials Testing / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D008689:Methacrylates / Q000138:chemical synthesis / Q000737:chemistry; D051379:Mice; D008856:Microscopy, Fluorescence; D010012:Osteogenesis / Q000187:drug effects / Q000235:genetics; D011073:Polyamines / Q000138:chemical synthesis / Q000737:chemistry; D066244:Proton Magnetic Resonance Spectroscopy; D060888:Real-Time Polymerase Chain Reaction
D003432:Cross-Linking Reagents; D020100:Hydrogels; D008689:Methacrylates; C531249:Poly(amidoamine); D011073:Polyamines
crosslinker; cytocompatible; photoreversible; self-healing hydrogel