Generation of Synthetic Copolymer Libraries by Combinatorial Assembly on Nucleic Acid Templates.
Recent advances in nucleic acid-templated copolymerization have expanded the scope of sequence-controlled synthetic copolymers beyond the molecular architectures witnessed in nature. This has enabled the power of molecular evolution to be applied to synthetic copolymer libraries to evolve molecular function ranging from molecular recognition to catalysis. This Review seeks to summarize different approaches available to generate sequence-defined monodispersed synthetic copolymer libraries using nucleic acid-templated polymerization. Key concepts and principles governing nucleic acid-templated polymerization, as well as the fidelity of various copolymerization technologies, will be described. The Review will focus on methods that enable the combinatorial generation of copolymer libraries and their molecular evolution for desired function.
Department of Chemistry, University of Georgia , 140 Cedar Street, Athens, Georgia 30602, United States.
Dehui
D
Kong
Department of Chemistry, University of Georgia , 140 Cedar Street, Athens, Georgia 30602, United States.
Wayland
W
Yeung
Department of Chemistry, University of Georgia , 140 Cedar Street, Athens, Georgia 30602, United States.
Ryan
R
Hili
D052157:Aptamers, Nucleotide / Q000737:chemistry; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D019020:Directed Molecular Evolution; D009696:Nucleic Acids / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry; D013698:Templates, Genetic
D052157:Aptamers, Nucleotide; D009696:Nucleic Acids; D009841:Oligonucleotides; D011108:Polymers
aptamer; combinatorial assembly; modified nucleic acids; molecular evolution; sequence-controlled; synthetic copolymer libraries