Versatile metal-organic framework-functionalized magnetic graphene nanoporous composites: As deft matrix for high-effective extraction and purification of the N-linked glycans.
The highly selective enrichment of N-linked glycans from complex biological sample is still very important but challenging task due to the ultra-low abundance, complicated structures and strong ion suppress effect caused by distractors such as proteins, peptides and salts. Here, we firstly present a novel metal-organic frameworks (MOFs)-functionalized magnetic nanoporous carbon-graphene composites (C-magG@ZIF-8) synthesized through a smart process. The obtained materials enjoy the unique properties including strong magnetic responsiveness, a large sum of graphitized carbon pore, remarkable biocompatibility and large specific surface area. By virtue of these unique properties, the C-magG@ZIF-8 composites displayed excellent selectivity and sensitivity, good recyclability and incredible size exclusion ability (roughly 2000 times) in the N-linked glycans analysis. Furthermore, 48 N-linked glycans were clearly identified from the normal human serum treated with the C-magG@ZIF-8. There is reason to believe that our smart strategy offers new possibilities for preparing the MOFs-functionalized composites for large-scale characterization of glycoproteomics by mass spectrometry analysis.
Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China.
Jiaxi
J
Wang
Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China.
Yanan
Y
Wang
Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China. Electronic address: mxgao@fudan.edu.cn.
Mingxia
M
Gao
Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China.
Xiangmin
X
Zhang
Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China.
Pengyuan
P
Yang
D005591:Chemical Fractionation / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D059346:Magnets / Q000737:chemistry*; D000073396:Metal-Organic Frameworks / Q000737:chemistry*; D008958:Models, Molecular; D008968:Molecular Conformation; D053761:Nanocomposites / Q000737:chemistry*; D058608:Nanopores; D009584:Nitrogen / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification*
D000073396:Metal-Organic Frameworks; D011134:Polysaccharides; D006108:Graphite; D009584:Nitrogen
Carbon-graphene composites; Extraction and purification; Mass spectrometry; Metal-organic framework-functionalized; N-linked glycan