αB-Crystallin interacts and attenuates the tyrosine phosphatase activity of Shp2 in cardiomyocytes under mechanical stress.
The small heat shock protein αB-Crystallin (CryAB, HspB5) and SH2 domain-containing tyrosine phosphatase 2 (Shp2) are important molecules in heart response to pathophysiological stress. Here we show that CryAB interacts with and potentially regulates Shp2 catalytic activity in stretched cardiomyocytes. Such an interaction requires CryAB oligomer to attenuate Shp2 activation. Stretched cardiomyocytes show a robust CryAB/Shp2 association accompanied by a reduction in the Shp2 phosphatase activity. Accordingly, CryAB knock-down in cardiomyocytes enhances Shp2 activity induced by mechanical stress. These results revealed a new role for CryAB, as a modulator of Shp2 phosphatase activity during a functionally relevant stimulus in cardiomyocytes.
Brazilian Biosciences National Laboratory, Campinas, Brazil.|Institute of Biology, State University of Campinas, Campinas, São Paulo, Brazil.
Danieli C
DC
Gonçalves
Brazilian Biosciences National Laboratory, Campinas, Brazil.
Talita M
TM
Marin
Medicine Department, Federal University of Juiz de Fora, Governador Valadares, Minas Gerais, Brazil.
Michelle B M
MB
Pereira
Institute of Biology, State University of Campinas, Campinas, São Paulo, Brazil.
Aline M
AM
Santos
Brazilian Biosciences National Laboratory, Campinas, Brazil.
Adriana F
AF
Paes Leme
Brazilian Biosciences National Laboratory, Campinas, Brazil.|School of Medicine, State University of Campinas, Campinas, São Paulo, Brazil.
Kleber G
KG
Franchini
D000818:Animals; D002478:Cells, Cultured; D003459:Crystallins / Q000235:genetics / Q000378:metabolism*; D004789:Enzyme Activation / Q000502:physiology; D055785:Gene Knockdown Techniques; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism*; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11 / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013314:Stress, Mechanical; D013312:Stress, Physiological / Q000502:physiology*
D003459:Crystallins; D008869:Microtubule-Associated Proteins; C530883:cryaB protein, rat; D054592:Protein Tyrosine Phosphatase, Non-Receptor Type 11
cardiomyocytes; chaperone; tyrosine phosphatase