Protective effects of Danggui Buxue Tang on renal function, renal glomerular mesangium and heparanase expression in rats with streptozotocin-induced diabetes mellitus.
Danggui Buxue Tang (DBT) is a simple combination of RadixAstragaliand RadixAngelica sinensis(5:1), with a variety pharmacological activities. In the present study, a single intravenous injection of 30 mg/kg streptozotocin and subsequent six weeks of high glucose diet in Sprague Dawley rats were used to induce diabetic nephropathy. Rats with diabetes mellitus showed increased levels of fasting blood glucose (FBG), blood urea nitrogen (BUN), serum creatinine (Scr), serum and urine β2-microglobulins (β2-MG), and type IV collagen (all P<0.05). DBT treatment significantly decreased the levels of FBG, BUN, Scr, serum and urine β2-MG, and type IV collagen. Furthermore, DBT treatment significantly and dose-dependently restored the ultrastructural injury, and reduced the expression of heparanase, compared with the vehicle (P<0.05). Therefore, DBT may be a novel therapeutic approach for the prevention and treatment of diabetic nephrology.
Department of Integrated Traditional Chinese and Western Medicine, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, P.R. China.
Tai-Sheng
TS
Ye
Department of Integrated Traditional Chinese and Western Medicine, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, P.R. China.
Ying-Wen
YW
Zhang
Department of Integrated Traditional Chinese and Western Medicine, The Third Hospital of Wuhan, Wuhan, Hubei 430060, P.R. China.
Xian-Mei
XM
Zhang
Danggui Buxue Tang; diabetic nephropathy; glomerular ultrastructure; heparanase; renal function