Lack of glucuronidation products of trans-resveratrol in plasma and urine of cats.
Resveratrol has generated interest in cats due to reported health benefits. Cats have low activity of β-glucuronidase, and we hypothesized they could not form two common resveratrol metabolites, resveratrol-3-O-glucuronide and resveratrol-4'-O-glucuronide. Resveratrol, 3 mg/cat/day, was given orally to intact male (n = 5) and female cats (n = 5) for 4 weeks. A control group (8 intact males) was used for comparison. Plasma and urine were collected weekly and analysed using high-pressure liquid chromatography coupled with tandem mass spectrometry. Resveratrol and resveratrol-3-O-sulphate, but no glucuronide metabolites, were detected in plasma and urine. Median (range 10-90th percentile) plasma resveratrol for control and treatment groups was 0.46 ng/ml (0.02-1.74 ng/ml) and 0.96 ng/ml (0.65-3.21 ng/ml). Median (range) plasma resveratrol-3-O-sulphate for control and treatment groups was 6.32 ng/ml (2.55-10.29 ng/ml) and 11.45 ng/ml (1.47-53.29 ng/ml). Plasma resveratrol differed from control in week 4, while plasma resveratrol-3-O-sulphate was different in all weeks (p < 0.05). Median (range) urine resveratrol for control and treatment groups was 0.28 ng/ml (0.05-1.59 ng/ml) and 19.98 ng/ml (8.44-87.54 ng/ml). Median (range) urine resveratrol-3-O-sulphate for control and treatment groups was 26.71 ng/ml (10.50-75.58 ng/ml) and 108.69 ng/ml (11.83-231.05 ng/ml). All time points for urine resveratrol and resveratrol-3-O-sulphate were significantly different from control (p < 0.05), except for weeks 1, 3 and 4 for resveratrol. The results support our hypothesis that cats are unlikely able to glucuronidate resveratrol, most likely due to a reduction in the activity of β-glucuronidase.
Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, CA, USA.
K
K
Burnett
Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, CA, USA.
B
B
Puschner
Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, CA, USA.
J J
JJ
Ramsey
Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, CA, USA.
Y
Y
Lin
Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, CA, USA.
A
A
Wei
Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, CA, USA.
A J
AJ
Fascetti
D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002415:Cats / Q000097:blood* / Q000652:urine*; D004032:Diet / Q000662:veterinary; D005260:Female; D008297:Male; D000077185:Resveratrol; D013047:Specific Pathogen-Free Organisms; D013267:Stilbenes / Q000097:blood / Q000378:metabolism* / Q000652:urine
D013267:Stilbenes; D000077185:Resveratrol
calorie restriction mimetic; feline; metabolism; polyphenol; sirtuin activator