FO-SPR based dextrose sensor using Ag/ZnO nanorods/GOx for insulinoma detection.
In this piece of work, a fiber optic sensor has been fabricated and characterized using surface plasmon resonance for dextrose sensing. The concentration range used in this study is for diagnosing the cases of hypoglycaemia especially in suppression tests of insulinoma. Insulinoma is a medical case in which the person is recognized being hypoglycaemic with the blood dextrose level falling down to 2.2mM or less. Thus, the sensor has been characterized for the dextrose concentration range of 0 mM-10mM including the cases of normal blood dextrose range. Coatings of silver layer and zinc oxide nanorods have been carried out on the bare core fiber with a dual role of zinc oxide followed by immobilization of glucose oxidase. A three stage optimization procedure has been adopted for the best performance of the sensor. Absorbance spectra have been plotted and peak absorbance wavelengths have been extracted for each concentration chosen along with the sensitivities. The results have been made conclusive with control experiments. The probe has also been tested on sample having blood serum to check the reliability of the sensor. The sensor shows better selectivity and response time along with its real time applications, online monitoring, remote sensing and reusability.
Department of Physics, Indian Institute of Technology Delhi, New Delhi 110016, India.
Sruthi P
SP
Usha
Department of Physics, Indian Institute of Technology Delhi, New Delhi 110016, India.
Anand M
AM
Shrivastav
Department of Physics, Indian Institute of Technology Delhi, New Delhi 110016, India. Electronic address: bdgupta@physics.iitd.ernet.in.
Banshi D
BD
Gupta
D015374:Biosensing Techniques / Q000295:instrumentation*; D001786:Blood Glucose / Q000032:analysis*; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D005336:Fiber Optic Technology / Q000295:instrumentation*; D005949:Glucose Oxidase / Q000737:chemistry; D006801:Humans; D007340:Insulinoma / Q000097:blood* / Q000175:diagnosis; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D055100:Optical Fibers; D015203:Reproducibility of Results; D012834:Silver / Q000737:chemistry*; D020349:Surface Plasmon Resonance; D015034:Zinc Oxide / Q000737:chemistry*
D001786:Blood Glucose; D004800:Enzymes, Immobilized; D012834:Silver; D005949:Glucose Oxidase; D015034:Zinc Oxide
Dextrose; Glucose oxidase; Hypoglycaemia; Insulinoma; Optical fiber; Sensor; Surface plasmon resonance; Zinc oxide nanorods