Activity of Extracts from Submerged Cultured Mycelium of Winter Mushroom, Flammulina velutipes (Agaricomycetes), on the Immune System In Vitro.
Extracts from submerged cultured mycelium of two strains of Flammulina velutipes, a popular culinary mushroom, were obtained by ultrasound and tested in vitro to determine their activity in innate immunity (monocytes/ macrophages). In addition, polyclonal antibodies against the extracts were produced. Both extracts have similar glycoproteins that contain mannose and glucose but have different glycoproteins with galactoseamine units. Two novel immunogenic glycoproteins with molecular weights of 32 and 25 kDa have been revealed. It is thought that these proteins are produced only by submerged cultured mycelium. Both extracts show immune-enhancing activity based on the significant modification of various parameters such as cytokine production, phagocytosis, and reactive oxygen species production.
Division de Ciencias Naturales y Exactas, Departamento de Biologia, Universidad de Guanajuato, Guanajuato, Guanajuato, México.
Svetlana
S
Kashina
Division de Ciencias Naturales y Exactas, Departamento de Biologia, Universidad de Guanajuato, Guanajuato, Guanajuato, México.
Lerida Liss Flores
LL
Villavicencio
Division de Ciencias de la Salud, Departamento de Ciencias Médicas, Universidad de Guanajuato, Leon, Guanajuato, México.
Silvio
S
Zaina
Division de Ingenierias, Departamento de Fisica Médica, Universidad de Guanajuato, Leon, Guanajuato, México.
Marco Balleza
MB
Ordaz
Division de Ciencias de la Salud, Departamento de Ciencias Médicas, Universidad de Guanajuato, Leon, Guanajuato, México.
Gloria Barbosa
GB
Sabanero
Centro de Investigacion y de Estudios Avanzados, Departamento de Infectomica y Patogénesis Molecular, Unidad Zacatenco, México, México.
Victor Tsutsumi
VT
Fujiyoshi
Division de Ciencias Naturales y Exactas, Departamento de Biologia, Universidad de Guanajuato, Guanajuato, Guanajuato, México.
Myrna Sabanero
MS
Lopez
D000818:Animals; D000906:Antibodies / Q000378:metabolism*; D001688:Biological Products / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002176:Candida albicans / Q000276:immunology; D055393:Flammulina / Q000737:chemistry*; D005656:Fungal Proteins / Q000276:immunology / Q000302:isolation & purification; D006023:Glycoproteins / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D007107:Immune System / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects; D008297:Male; D009000:Monocytes / Q000187:drug effects; D025282:Mycelium / Q000737:chemistry; D010587:Phagocytosis / Q000187:drug effects; D011817:Rabbits; D017382:Reactive Oxygen Species / Q000378:metabolism
D000906:Antibodies; D001688:Biological Products; D005656:Fungal Proteins; D006023:Glycoproteins; D017382:Reactive Oxygen Species