MDC1-AS, an antisense long noncoding RNA, regulates cell proliferation of glioma.
BACKGROUND
Growing number of long noncoding RNAs (lncRNAs) are emerging as new modulators in cancer origination and progression. A lncRNA, mediator of DNA damage checkpoint protein 1antisense RNA (MDC1-AS), with unknown function, is the antisense transcript of tumor suppressor MDC1.


METHODS
In this study, we investigated the expression pattern and functional role of lncRNA MDC1-AS in glioma by using real time PCR and gain-/loss-of-function studies.


RESULTS
The results showed that the expression levels of lncRNA MDC1-AS and MDC1 were significantly downregulated in glioma tissues compared with normal brain tissues, and in glioma cell lines U87MG, U251 and HEB. Overexpression of MDC1-AS resulted in significant inhibition of cell proliferation and cell cycle in U87MG and U251. We also found that MDC1-AS expression was positively correlated with MDC1 expression. In addition, the inhibitory role of MDC1-AS was remarkably diminished when MDC1 was knockdown.


CONCLUSIONS
Together, the results suggest that MDC1-AS is a novel tumor suppressor through up-regulation of its antisense tumor-suppressing gene MDC1 in glioma and leads us to propose that MDC1-AS may serve as a potential biomarker and therapeutic target for glioma.
Department of Neurosurgery, Ji'nan Central Hospital Affiliated to Shandong University, Ji'nan 250013, PR China.
Hongsheng
H
Yue
Department of Neurosurgery, Ji'nan Central Hospital Affiliated to Shandong University, Ji'nan 250013, PR China.
Jie
J
Zhu
Department of Neurosurgery, Shanghe County People's Hospital, Ji'nan 251600, PR China.
Shugang
S
Xie
Nursing Department, Shandong Cancer Hospital and Institute, Ji'nan 250117, PR China.
Fangfang
F
Li
Nursing Department, Ji'nan Vocational College of Nursing, Ji'nan 250102, PR China. Electronic address: qunxu199@163.com.
Qun
Q
Xu
D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology*; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism
D062085:RNA, Long Noncoding; C000613812:long non-coding RNA MDC1-AS, human
Cell proliferation; Glioma; Long non-coding RNA MDC1-AS; MDC1