Direct Adsorption of Anti-CD34 Antibodies on the Nano-Porous Stent Surface to Enhance Endothelialization.
BACKGROUND
In-stent restenosis following the insertion of conventional drug-eluting stent has become an extremely serious problem due to coating techniques, with polymer matrices used to bind biological ingredients to the stent surface. However, several studies have indicated that new pro-healing technique could prevent stent thrombosis that can be caused by conventional drug-eluting stents.


METHODS
A novel method of attaching anti-CD34 antibodies directly on the porous surface of a 316L stainless steel bare metal stent was developed in this study, which achieved both high stability of attached anti-CD34 antibodies on the metal stent surface and high antibody activity for stem cell capture.


RESULTS
The in vitro and in vivo experimental results indicated that the new stent with directly coupled anti-CD34 antibodies can efficiently enhance stent endothelialization.


CONCLUSIONS
This study indicates that we have developed a unique method of attaching anti-CD34 antibodies directly on the porous surface of a 316L stainless steel bare metal stent, which provides a novel polymer-free approach for developing pro-healing stents.
Department of Cardiovascular Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052;
Guowei
G
Fu
State Key Laboratory of Natural and Biomimetic Drugs and Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University, Beijing 100191;
Zhanjiang
Z
Yu
Lepu Medical Technology (Beijing) Co., Ltd, Beijing 100022;
Yongqiang
Y
Chen
Department of Cardiology, The General Hospital of the People's Liberation Army, Beijing 100853, P. R. China.
Yundai
Y
Chen
Department of Cardiology, The General Hospital of the People's Liberation Army, Beijing 100853, P. R. China.
Feng
F
Tian
State Key Laboratory of Natural and Biomimetic Drugs and Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University, Beijing 100191;
Xiaoda
X
Yang
Adsorption; Endothelialization; Nano-porous surface; Self-assembly