Complexity and Specificity of the Neutrophil Transcriptomes in Juvenile Idiopathic Arthritis.
NIH projects such as ENCODE and Roadmap Epigenomics have revealed surprising complexity in the transcriptomes of mammalian cells. In this study, we explored transcriptional complexity in human neutrophils, cells generally regarded as nonspecific in their functions and responses. We studied distinct human disease phenotypes and found that, at the gene, gene isoform, and miRNA level, neutrophils exhibit considerable specificity in their transcriptomes. Thus, even cells whose responses are considered non-specific show tailoring of their transcriptional repertoire toward specific physiologic or pathologic contexts. We also found that miRNAs had a global impact on neutrophil transcriptome and are associated with innate immunity in juvenile idiopathic arthritis (JIA). These findings have important implications for our understanding of the link between genes, non-coding transcripts and disease phenotypes.
Center for Computational Research, New York State Center of Excellence in Bioinformatics &Life Sciences, State University of New York at Buffalo, Buffalo, NY 14260, USA.|Department of Ophthalmology, Department of Biostatistics, Department of Medicine, State University of New York at Buffalo, Buffalo, NY 14260, USA.|SUNY Eye Institute, Buffalo, NY 14260, USA.
Zihua
Z
Hu
Department of Pediatrics, Division of Allergy/Immunology/Rheumatology, University at Buffalo, Buffalo, NY 14203, USA.
Kaiyu
K
Jiang
Arthritis &Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA.
Mark Barton
MB
Frank
Department of Pediatrics, Division of Allergy/Immunology/Rheumatology, University at Buffalo, Buffalo, NY 14203, USA.
Yanmin
Y
Chen
Department of Pediatrics, Division of Allergy/Immunology/Rheumatology, University at Buffalo, Buffalo, NY 14203, USA.|Graduate Program in Genetics, Genomics, &Bioinformatics, University at Buffalo, Buffalo, NY 14203, USA.
James N
JN
Jarvis
D000293:Adolescent; D001171:Arthritis, Juvenile / Q000235:genetics*; D016022:Case-Control Studies; D005091:Exons; D053263:Gene Regulatory Networks; D006801:Humans; D035683:MicroRNAs / Q000235:genetics; D009504:Neutrophils / Q000378:metabolism*; D059467:Transcriptome
D035683:MicroRNAs