A novel α-glucosidase inhibitory constituent from Uncaria gambir.
Bioactivity-guided fractionation of an aqueous methanolic extract of manufactured gambir product from Uncaria gambir with in vitro α-glucosidase inhibitory activity was performed to isolate a novel prenyl resorcinol derivative (1) together with seven known compounds, including two flavone glycosides (2, 3), three catechin analogues (4-6), and two simple phenolics (7, 8). Structures of the isolated compounds were determined by analysis of physical and spectroscopic data (NMR, UV, [α]D, and MS). All isolates were evaluated for in vitro α-glucosidase inhibitory activity. Among the compounds, novel compound 1, possessing an unprecedented spirocyclopropane ring in the molecule, showed the most potent α-glucosidase inhibitory activity in this assay. On the other hand, compounds 4 and 7 showed less potent inhibitory effects in this same bioassay, with half-maximal inhibitory concentration values of 17.3 ± 1.0 μM and 27.0 ± 0.9 μM, respectively.
Department of Food Science and Biotechnology, Daegu University, 201 Daegudae-ro, Gyeongsan-si, Gyeongsangbuk-do, 38453, Republic of Korea. skyey7@daegu.ac.kr.
Tae Hoon
TH
Kim
D065089:Glycoside Hydrolase Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D006027:Glycosides / Q000737:chemistry; D015394:Molecular Structure; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000494:pharmacology*; D013141:Spiro Compounds / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D032070:Uncaria / Q000737:chemistry*; D000520:alpha-Glucosidases / Q000187:drug effects*
D065089:Glycoside Hydrolase Inhibitors; D006027:Glycosides; D010636:Phenols; D010936:Plant Extracts; D013141:Spiro Compounds; C000615793:gambicarin; D000520:alpha-Glucosidases
Anti-diabetes; Flavone glycosides; Prenyl resorcinol derivative; Uncaria gambir; α-Glucosidase