Monitoring of antifungal drugs in biological samples using ultrasonic-assisted supramolecular dispersive liquid-liquid microextraction based on solidification of a floating organic droplet.
A new method for the simultaneous determination of the three antifungal drugs using ultrasonic-assisted supramolecular dispersive liquid-liquid microextraction based on solidification of a floating organic droplet (UASMDLLME-SFO) was proposed. The supramolecular solvents produced from reversed micelles of 1-dodecanol (extraction solvent) in tetrahydrofuran (THF) were injected into the aqueous sample solution. Reverse micelle coacervates were produced in situ through self-assembly processes. The antifungal drugs were extracted from the aqueous sample into a supramolecular solvent. Sonication accelerated the mass transfer of the target analytes into the supramolecular solvent phase and enhanced the dispersion process. Some parameters affecting the extraction efficiency such as type and volume of the extraction solvent, pH, volume of the disperser solvent and ultrasound extraction time were investigated. Under optimum conditions, the limits of detections for ketoconazole, clotrimazole and miconazole ranged from 0.08 to 1.3μgL(-1) and the relative standard deviations (RSDs, n=5)<6% were obtained. The method was successfully applied for preconcentration of the three drugs in biological and water samples.
Department of Chemistry, Payame Noor University, P.O. BOX 19395-3697, Tehran, Iran. Electronic address: maryams77@gmail.com.
Maryam
M
Ezoddin
Department of Radiopharmacy and Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Khosrou
K
Abdi
D000935:Antifungal Agents / Q000032:analysis* / Q000097:blood* / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007851:Dodecanol / Q000737:chemistry; D060766:Drinking Water / Q000032:analysis*; D005663:Furans / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D059627:Liquid Phase Microextraction / Q000379:methods*; D008823:Micelles; D012997:Solvents / Q000737:chemistry; D013010:Sonication / Q000379:methods; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000097:blood* / Q000652:urine
D000935:Antifungal Agents; D060766:Drinking Water; D005663:Furans; D008823:Micelles; D012997:Solvents; D014874:Water Pollutants, Chemical; D007851:Dodecanol; C018674:tetrahydrofuran
Antifungal drugs; Dispersive liquid–liquid microextraction; Solidification; Sonication; Supramolecular