Molecular identification and biological characterization of a new potyvirus in lettuce.
A potyvirus causing necrosis and leaf distortion on lettuce was found in the Lazio region of Italy. Host range analysis showed its ability to infect only Chenopodium quinoa and C. amaranticolor in addition to some lettuce cultivars. The virus could be transmitted by aphids of the species Myzus persicae. The complete 9829-nt genome was characterized. BLAST analysis of sequence of the complete encoded polyprotein showed that the most closely related virus is asparagus virus 1, with 52 % amino acid sequence identity. These results suggest that this virus should be considered a member of a new species in the genus Potyvirus.
Istituto per la Protezione Sostenibile delle Piante, Sede di Torino, CNR, Strada delle Cacce 73, 10135, Turin, Italy.
Marina
M
Ciuffo
Nunhems Italy SRL, Bayer Crop Science Vegetable Seeds, Via Ghiarone 2, 40019, Sant'Agata Bolognese, BO, Italy.
Marco
M
Mammella
Istituto per la Protezione Sostenibile delle Piante, Sede di Torino, CNR, Strada delle Cacce 73, 10135, Turin, Italy.
Marta
M
Vallino
Istituto per la Protezione Sostenibile delle Piante, Sede di Torino, CNR, Strada delle Cacce 73, 10135, Turin, Italy.
Piero
P
Caciagli
Istituto per la Protezione Sostenibile delle Piante, Sede di Torino, CNR, Strada delle Cacce 73, 10135, Turin, Italy. massimo.turina@ipsp.cnr.it.
Massimo
M
Turina
D027462:Chenopodium / Q000821:virology; D016679:Genome, Viral; D018545:Lactuca / Q000821:virology*; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D017800:Potyvirus / Q000235:genetics* / Q000302:isolation & purification*