Rapid diagnosis of lymph node metastasis in breast cancer using a new fluorescent method with γ-glutamyl hydroxymethyl rhodamine green.
Sentinel lymph node biopsy is performed as a standard procedure in breast cancer surgery, and the development of quick and simple methods to detect metastatic lesions is in high demand. Here, we validated a new fluorescent method using γ-glutamyl hydroxymethyl rhodamine green to diagnose metastatic lymph nodes in breast cancer. One hundred and forty-nine lymph nodes from 38 breast cancer patients were evaluated in this study. Comparison of fluorescent and pathological images showed that this fluorescent method was successful for visualizing breast cancer cells in lymph nodes. This method had a sufficiently high sensitivity (97%), specificity (79%) and negative predictive value (99%) to render it useful for an intraoperative diagnosis of cancer. These preliminary findings suggest that this novel method is useful for distinguishing non-cancerous specimens from those in need of careful examination and could help save time and cost for surgeons and pathologists.
Department of Surgery, Breast and Thyroid Surgery, Kagoshima University, Graduate School of Medical and Dental Sciences, 8-35-1, Kagoshima-City, Kagoshima 890-8520, Japan.|Department of Digestive Surgery, Breast and Thyroid Surgery, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima 890-8520, Japan.
Yoshiaki
Y
Shinden
Department of Surgery, Breast and Thyroid Surgery, Kagoshima University, Graduate School of Medical and Dental Sciences, 8-35-1, Kagoshima-City, Kagoshima 890-8520, Japan.|Department of Surgery and Sciences, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
Hiroki
H
Ueo
Department of Pathology, Kyushu University Beppu Hospital, 4546 Tsurumihara, Beppu 874-0838, Japan.
Taro
T
Tobo
Department of Pathology, Oita University, Yufu 879-5593, Japan.
Ayako
A
Gamachi
Department of Pathology, Kyushu University Beppu Hospital, 4546 Tsurumihara, Beppu 874-0838, Japan.
Mitsuaki
M
Utou
Department of Surgery, Breast and Thyroid Surgery, Kagoshima University, Graduate School of Medical and Dental Sciences, 8-35-1, Kagoshima-City, Kagoshima 890-8520, Japan.
Hisateru
H
Komatsu
Department of Surgery, Breast and Thyroid Surgery, Kagoshima University, Graduate School of Medical and Dental Sciences, 8-35-1, Kagoshima-City, Kagoshima 890-8520, Japan.
Sho
S
Nambara
Department of Surgery, Breast and Thyroid Surgery, Kagoshima University, Graduate School of Medical and Dental Sciences, 8-35-1, Kagoshima-City, Kagoshima 890-8520, Japan.
Tomoko
T
Saito
Department of Surgery, Breast and Thyroid Surgery, Kagoshima University, Graduate School of Medical and Dental Sciences, 8-35-1, Kagoshima-City, Kagoshima 890-8520, Japan.
Masami
M
Ueda
Department of Surgery, Breast and Thyroid Surgery, Kagoshima University, Graduate School of Medical and Dental Sciences, 8-35-1, Kagoshima-City, Kagoshima 890-8520, Japan.
Hidenari
H
Hirata
Department of Surgery, Breast and Thyroid Surgery, Kagoshima University, Graduate School of Medical and Dental Sciences, 8-35-1, Kagoshima-City, Kagoshima 890-8520, Japan.
Shotaro
S
Sakimura
Department of Surgery, Breast and Thyroid Surgery, Kagoshima University, Graduate School of Medical and Dental Sciences, 8-35-1, Kagoshima-City, Kagoshima 890-8520, Japan.
Yuki
Y
Takano
Department of Surgery, Breast and Thyroid Surgery, Kagoshima University, Graduate School of Medical and Dental Sciences, 8-35-1, Kagoshima-City, Kagoshima 890-8520, Japan.
Ryutaro
R
Uchi
Department of Surgery, Breast and Thyroid Surgery, Kagoshima University, Graduate School of Medical and Dental Sciences, 8-35-1, Kagoshima-City, Kagoshima 890-8520, Japan.
Junji
J
Kurashige
Department of Surgery, Breast and Thyroid Surgery, Kagoshima University, Graduate School of Medical and Dental Sciences, 8-35-1, Kagoshima-City, Kagoshima 890-8520, Japan.
Sayuri
S
Akiyoshi
Department of Surgery, Breast and Thyroid Surgery, Kagoshima University, Graduate School of Medical and Dental Sciences, 8-35-1, Kagoshima-City, Kagoshima 890-8520, Japan.
Tomohiro
T
Iguchi
Department of Surgery, Breast and Thyroid Surgery, Kagoshima University, Graduate School of Medical and Dental Sciences, 8-35-1, Kagoshima-City, Kagoshima 890-8520, Japan.
Hidetoshi
H
Eguchi
Department of Surgery, Breast and Thyroid Surgery, Kagoshima University, Graduate School of Medical and Dental Sciences, 8-35-1, Kagoshima-City, Kagoshima 890-8520, Japan.
Keishi
K
Sugimachi
Ueo Breast Surgery Hospital, Oita 870-0854, Japan.
Yoko
Y
Kubota
Ueo Breast Surgery Hospital, Oita 870-0854, Japan.
Yuichiro
Y
Kai
Ueo Breast Surgery Hospital, Oita 870-0854, Japan.
Kenji
K
Shibuta
Department of Digestive Surgery, Breast and Thyroid Surgery, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima 890-8520, Japan.
Yuko
Y
Kijima
Department of Digestive Surgery, Breast and Thyroid Surgery, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima 890-8520, Japan.
Heiji
H
Yoshinaka
Department of Digestive Surgery, Breast and Thyroid Surgery, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima 890-8520, Japan.
Shoji
S
Natsugoe
Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita 565-0871, Japan.
Masaki
M
Mori
Department of Surgery and Sciences, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
Yoshihiko
Y
Maehara
Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.
Masayo
M
Sakabe
Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.
Mako
M
Kamiya
Signal Processing and Instrumentation Section, Division of Computational Bioscience, Center of Information Technology, National Institutes of Health, Bethesda, MD 20892-5624, USA.
John W
JW
Kakareka
Signal Processing and Instrumentation Section, Division of Computational Bioscience, Center of Information Technology, National Institutes of Health, Bethesda, MD 20892-5624, USA.
Thomas J
TJ
Pohida
Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-1088, USA.
Peter L
PL
Choyke
Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-1088, USA.
Hisataka
H
Kobayashi
Ueo Breast Surgery Hospital, Oita 870-0854, Japan.
Hiroaki
H
Ueo
Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.|CREST, AMED, Tokyo 100-0004, Japan.
Yasuteru
Y
Urano
Department of Surgery, Breast and Thyroid Surgery, Kagoshima University, Graduate School of Medical and Dental Sciences, 8-35-1, Kagoshima-City, Kagoshima 890-8520, Japan.
Koshi
K
Mimori
D000368:Aged; D001943:Breast Neoplasms / Q000473:pathology*; D018270:Carcinoma, Ductal, Breast / Q000473:pathology*; D018275:Carcinoma, Lobular / Q000473:pathology; D004151:Dipeptides / Q000737:chemistry*; D005260:Female; D006801:Humans; D008198:Lymph Nodes / Q000473:pathology; D008207:Lymphatic Metastasis / Q000000981:diagnostic imaging*; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D009362:Neoplasm Metastasis; D012235:Rhodamines / Q000737:chemistry*; D012680:Sensitivity and Specificity; D021701:Sentinel Lymph Node Biopsy
D004151:Dipeptides; D012235:Rhodamines; C583513:glutamyl-phenylalanine hydroxymethyl rhodamine green