Lactoferrin Enhanced Apoptosis and Protected Against Thioacetamide-Induced Liver Fibrosis in Rats.
BACKGROUND
Liver fibrosis is the common pathologic consequence of all chronic liver diseases.


OBJECTIVE
Lactoferrin (Lf) was investigated for its possible hepatoprotective effect against thioacetamide (TAA)-induced liver fibrosis rat model.


METHODS
Rats received TAA (200 mg/kg/biweekly, ip) for four successive weeks. Lf (200 mg/kg/day, p.o.) or vehicle (VHC) was administered for one month before and another month during TAA injection. Body weight and mortality rate were assessed during the month of TAA-intoxication. Thereafter, serum and liver tissues were analyzed for liver function, oxidative, fibrotic and apoptotic markers.


RESULTS
Lf conserved rats against TAA-induced body weight-loss and mortality. Preservation of serum albumin, alkaline phosphatase and total bilirubin levels was also observed. Lf also protected rats against TAA-induced decrease in reduced glutathione and increase in malondialdehyde liver contents. Normal liver contents of hydroxyproline, nuclear factor kappa B and alpha fetoprotein; as markers of fibrosis; were increased with TAA and conserved with Lf-TAA. Lf maintained the normal architecture of the liver and immunohistochemical findings revealed increase in apoptotic bodies compared to TAA that favored necrosis.


CONCLUSIONS
In conclusion, Lf improved liver function, reduced oxidative stress and liver fibrosis, and enhanced apoptosis in rats with liver fibrosis, suggesting it to have useful therapeutic potential in patients with liver fibrosis.
National Research Center, Medical Division, Pharmacology Department, Giza, Egypt.
Alyaa
A
Hessin
National Research Center, Medical Division, Pharmacology Department, Giza, Egypt.
Rehab
R
Hegazy
Cairo University, Faculty of Veterinary Medicine, Pathology Department, Giza, Egypt.
Azza
A
Hassan
National Research Center, Medical Division, Pharmacology Department, Giza, Egypt.
Nemat
N
Yassin
Cairo University, Faculty of Pharmacy, Pharmacology and Toxicology Department, Cairo, Egypt.
Sanaa
S
Kenawy
Alpha fetoprotein; Apoptosis; Lactoferrin; Liver fibrosis; NF-κB; Thioacetamide