Cellular dissection of zebrafish hematopoiesis.
Zebrafish as a model system have been instrumental in understanding early vertebrate development, especially of the hematopoietic system. The external development of zebrafish and their genetic amenability have allowed in-depth studies of multiple blood cell types and their respective genetic regulation. This chapter highlights some new data in zebrafish hematopoiesis regarding primitive and definitive hematopoiesis in the embryonic and adult fish, allowing the isolation of prospective progenitor subsets. It also highlights assays developed to examine the function of these progenitors in vivo and in vitro, allowing an evolutionary understanding of the hematopoietic system and how zebrafish can be better utilized as a model system for a multitude of hematopoietic disorders.
California State University, Chico, Chico, CA, United States.
D L
DL
Stachura
University of California, San Diego, San Diego, CA, United States.
D
D
Traver
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002469:Cell Separation; D004195:Disease Models, Animal; D004625:Embryo, Nonmammalian / Q000378:metabolism; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D015027:Zebrafish / Q000254:growth & development / Q000502:physiology*
Hematopoietic cell enrichment and transplantation; Zebrafish adult and developmental hematopoiesis; Zebrafish clonal hematopoietic cell assays; Zebrafish in vitro cell culture