Grueneisen relaxation photoacoustic microscopy in vivo.
Grueneisen relaxation photoacoustic microscopy (GR-PAM) can achieve optically defined axial resolution, but it has been limited to ex vivo demonstrations so far. Here, we present the first in vivo image of a mouse brain acquired with GR-PAM. To induce the GR effect, an intensity-modulated continuous-wave laser was employed to heat absorbing objects. In phantom experiments, an axial resolution of 12.5  μm was achieved, which is sixfold better than the value achieved by conventional optical-resolution PAM. This axial-resolution improvement was further demonstrated by imaging a mouse brain in vivo, where significantly narrower axial profiles of blood vessels were observed. The in vivo demonstration of GR-PAM shows the potential of this modality for label-free and high-resolution anatomical and functional imaging of biological tissues.
Jun
J
Ma
Junhui
J
Shi
Pengfei
P
Hai
Yong
Y
Zhou
Lihong V
LV
Wang
D000818:Animals; D001921:Brain / Q000098:blood supply / Q000000981:diagnostic imaging; D007834:Lasers; D051379:Mice; D008853:Microscopy; D061848:Optical Imaging / Q000379:methods*; D061088:Photoacoustic Techniques