Evaluation of a time efficient immunization strategy for anti-PAH antibody development.
The development of monoclonal antibodies (mAb) with affinity to small molecules can be a time-consuming process. To evaluate shortening the time for mAb production, we examined mouse antisera at different time points post-immunization to measure titer and to evaluate the affinity to the immunogen PBA (pyrene butyric acid). Fusions were also conducted temporally to evaluate antibody production success at various time periods. We produced anti-PBA antibodies 7 weeks post-immunization and selected for anti-PAH reactivity during the hybridoma screening process. Moreover, there were no obvious sensitivity differences relative to antibodies screened from a more traditional 18-week schedule. Our results demonstrate a more time efficient immunization strategy for anti-PAH antibody development that may be applied to other small molecules.
a Aquatic Health Sciences , Virginia Institute of Marine Science, College of William & Mary , Gloucester Point , Virginia , USA.
Xin
X
Li
a Aquatic Health Sciences , Virginia Institute of Marine Science, College of William & Mary , Gloucester Point , Virginia , USA.
Stephen L
SL
Kaattari
a Aquatic Health Sciences , Virginia Institute of Marine Science, College of William & Mary , Gloucester Point , Virginia , USA.
Mary Ann
MA
Vogelbein
a Aquatic Health Sciences , Virginia Institute of Marine Science, College of William & Mary , Gloucester Point , Virginia , USA.
Michael A
MA
Unger
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D006433:Hemocyanins / Q000008:administration & dosage / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011084:Polycyclic Aromatic Hydrocarbons / Q000276:immunology*; D011721:Pyrenes / Q000008:administration & dosage / Q000276:immunology; D013997:Time Factors
D000911:Antibodies, Monoclonal; D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin
PAH; affinity; anti-sera titer; fusion; immunization strategy; mAbs