Pyrostegia venusta (Ker Gawl.) Miers Crude Extract and Fractions: Prevention of Dental Biofilm Formation and Immunomodulatory Capacity.
BACKGROUND
Caries and periodontal diseases remain as important diseases in the Brazilian population. One important pathogen associated with this situation is Streptococcus mutans and other important factor is this pathogen's ability to adhere firmly to the tooth surface leading to dental biofilm formation and caries development.


OBJECTIVE
Determine the antibacterial and other biological activities of P. venusta related to its potential to be used in the treatment of caries and periodontal disease.


METHODS
The growth inhibition by P. venusta of Streptococcus mutans, S. mitis, S. oralis and Candida albicans was determined using the broth microdilution method. In addition, the effect of the samples in adherence and reducing production of acids by S. mutans, and germ-tube formation of C. albicans was analysed. The Nitric Oxide (NO) production and cytotoxicity of P. venusta to peripheral blood mononuclear cells (PBMC) and RAW 264.7 Cell Line Murine Macrophage from Blood were assessed.


RESULTS
The crude extract (CE) and ethyl-acetate (AF) and n-butanol (BF) fractions showed antibacterial activity. The ethyl-acetate (AF) fraction showed the highest inhibition percentage against the adherence of S. mutans and C. albicans cells without budding, beyond NO production inhibition. There was not any cytotoxicity in the murine macrophages RAW 264.7 cells.


CONCLUSIONS
Our results suggest that P. venusta presents potential to be used as a preliminary source of compounds that can provide helpful activity when used in prophylaxis or treatment of caries or periodontal disease.


CONCLUSIONS
Biological activities of Pyrostegia venusta and its potential for use in formulations for the prevention of oral diseases. Abbreviations used: NO: Nitric oxide, PBMC: Peripheral blood mononuclear cells, CE: Crude extract, AF: Ethyl-acetate fraction, BF: n-butanol fraction, HF: Hexane fraction, WF: Water fraction, MIC: Minimum inhibitory concentration, MBC: Minimum bactericidal concentration, ATCC: American Type Culture Collection, CFU: Colony-forming units, BHI: Brain heart infusion, RPMI: Roswell Park Memorial Institute, MOPS: 3-(N-morpholino)propanesulfonic acid, DMEM: Dulbecco's modified Eagle's médium, LPS: Lipopolysacharide, MTT: 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide, OD: Optical density, AC: Acteoside.
Department of Post-Graduation Program in Pharmaceutical Sciences, Institute of Health Sciences, Federal University of Pará, Augusto Corrêa Avenue, No. 01, University Campus of Guamá, Belém, Pará CEP 67150-110, Brazil.
Mayara Brito
MB
de Sousa
Department of Post-Graduation Program in Pharmaceutical Sciences, Institute of Health Sciences, Federal University of Pará, Augusto Corrêa Avenue, No. 01, University Campus of Guamá, Belém, Pará CEP 67150-110, Brazil; Laboratory of Research and Development in Pharmaceutical and Cosmetic, College of Pharmacy, Institute of Health Sciences, Federal University of Pará, Augusto Corrêa Avenue, No. 01, University Campus of Guamá, Belém, Pará CEP 67150-110, Brazil.
José Otávio Carrera Silva
JO
Júnior
Department of Post-Graduation Program in Pharmaceutical Sciences, Institute of Health Sciences, Federal University of Pará, Augusto Corrêa Avenue, No. 01, University Campus of Guamá, Belém, Pará CEP 67150-110, Brazil.
Wagner Luiz Ramos
WL
Barbosa
Department of Post-Graduation Program in Pharmaceutical Sciences, Institute of Health Sciences, Federal University of Pará, Augusto Corrêa Avenue, No. 01, University Campus of Guamá, Belém, Pará CEP 67150-110, Brazil.
Erika
E
da Silva Valério
College of Pharmacy, Institute of Health Sciences, Federal University of Pará, Augusto Corrêa Avenue, No. 01, University Campus of Guamá, Belém, Pará CEP 67150-110, Brazil.
Andriele
A
da Mata Lima
Laboratory of Bioactive Products, School of Pharmacy, Federal University of Rio de Janeiro, Campus Macaé, Polo Novo Cavaleiros - IMCT, Alcides da Conceição Street, 159 Novo Cavaleiros, CEP 27933-378, Brazil.
Marlon Heggdorne
MH
de Araújo
Laboratory of Bioactive Products, School of Pharmacy, Federal University of Rio de Janeiro, Campus Macaé, Polo Novo Cavaleiros - IMCT, Alcides da Conceição Street, 159 Novo Cavaleiros, CEP 27933-378, Brazil.
Michelle Frazão
MF
Muzitano
Department of Post-Graduation Program in Pharmaceutical Sciences, Health Sciences Center, State University of Maringá, Av. Colombo, 5790, Maringá, Paraná CEP 87020-900, Brazil.
Celso Vataru
CV
Nakamura
Department of Post-Graduation Program in Pharmaceutical Sciences, Health Sciences Center, State University of Maringá, Av. Colombo, 5790, Maringá, Paraná CEP 87020-900, Brazil.
João Carlos Palazzo
JC
de Mello
Department of Post-Graduation Program in Pharmaceutical Sciences, Institute of Health Sciences, Federal University of Pará, Augusto Corrêa Avenue, No. 01, University Campus of Guamá, Belém, Pará CEP 67150-110, Brazil; Pharmacy Course, Federal University of Rio de Janeiro, Campus Macaé, Av. Aluízio da Silva Gomes, 50 Granja dos Cavaleiros, CEP 27930-560, Macaé, Rio de Janeiro, CEP 87020-900, Brazil.
Francisco Martins
FM
Teixeira
Anti-bacterial agents; Candida spp.; Streptococcus spp; caries; periodontal disease