Development and In Vitro Evaluation of a Novel Drug Delivery System (Albumin Microspheres Containing Liposomes) Applied to Vancomycin.
A pharmaceutical vehicle based on the encapsulation of liposomes with unmodified albumin has been designed, formulated, and in vitro characterized. Microscopy was used to investigate particle morphology and dynamic light scattering to determine the size and zeta potential. Vancomycin was selected as a model drug for water-soluble and moderately albumin-bound products. The results indicated that regardless of the zeta potential of the liposomes these can be trapped within albumin microspheres. The zeta potential, drug entrapment efficacy, and drug delivery profile of the resulting microspheres were found to depend on the liposome composition and the conditions of flocculation. The protein concentration was observed to influence drug entrapment efficiency (from 13.17 ± 5.0% to 61.27 ± 4.54%), as did the zeta potential of the microspheres, which was also seen to depend on the initial charge of the liposomes. The relationship between the microsphere zeta potential or entrapment efficacy and the protein concentration used for flocculation was established. Regarding drug delivery, differences between microspheres prepared from cationic or anionic liposomes were observed. The combination of liposome versatility together with the drug-binding ability of albumin provides to a vehicle with multiple choices for theranostic delivery.
Pharmaceutical Sciences Department, University of Salamanca, Salamanca 37007, Spain; Institute of Biomedical Research of Salamanca (IBSAL), Salamanca 37007, Spain. Electronic address: mariajosedj@usal.es.
María José
MJ
de Jesús Valle
Physical Chemistry Department, University of Salamanca, Salamanca 37008, Spain.
David
D
López Díaz
Physical Chemistry Department, University of Salamanca, Salamanca 37008, Spain.
Mercedes
M
Velázquez Salicio
Pharmaceutical Sciences Department, University of Salamanca, Salamanca 37007, Spain; Institute of Biomedical Research of Salamanca (IBSAL), Salamanca 37007, Spain.
Amparo
A
Sánchez Navarro
D000418:Albumins / Q000737:chemistry*; D000465:Algorithms; D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D004337:Drug Carriers; D004339:Drug Compounding; D016503:Drug Delivery Systems; D008081:Liposomes; D008863:Microspheres; D010316:Particle Size; D000068936:Theranostic Nanomedicine; D014640:Vancomycin / Q000008:administration & dosage*
D000418:Albumins; D000900:Anti-Bacterial Agents; D004337:Drug Carriers; D008081:Liposomes; D014640:Vancomycin
albumin; coacervation; liposomes; microencapsulation; microspheres; protein binding