A restricted period for formation of outer subventricular zone defined by Cdh1 and Trnp1 levels.
The outer subventricular zone (OSVZ) is a germinal layer playing key roles in the development of the neocortex, with particular relevance in gyrencephalic species such as human and ferret, where it contains abundant basal radial glia cells (bRGCs) that promote cortical expansion. Here we identify a brief period in ferret embryonic development when apical RGCs generate a burst of bRGCs that become founders of the OSVZ. After this period, bRGCs in the OSVZ proliferate and self-renew exclusively locally, thereby forming a self-sustained lineage independent from the other germinal layers. The time window for the brief period of OSVZ bRGC production is delineated by the coincident downregulation of Cdh1 and Trnp1, and their upregulation reduces bRGC production and prevents OSVZ seeding. This mechanism in cortical development may have key relevance in brain evolution and disease.
Instituto de Neurociencias, Consejo Superior de Investigaciones Científicas and Universidad Miguel Hernández, Sant Joan d'Alacant 03550, Spain.
Maria Ángeles
MÁ
Martínez-Martínez
Instituto de Neurociencias, Consejo Superior de Investigaciones Científicas and Universidad Miguel Hernández, Sant Joan d'Alacant 03550, Spain.
Camino
C
De Juan Romero
Instituto de Neurociencias, Consejo Superior de Investigaciones Científicas and Universidad Miguel Hernández, Sant Joan d'Alacant 03550, Spain.
Virginia
V
Fernández
Instituto de Neurociencias, Consejo Superior de Investigaciones Científicas and Universidad Miguel Hernández, Sant Joan d'Alacant 03550, Spain.
Adrián
A
Cárdenas
Helmholtz Center Munich, German Research Center for Environmental Health, Institute for Stem Cell Research, 85764 Neuherberg, Germany.|Physiological Genomics, University of Munich, 80336 Munich, Germany.|SYNERGY, Excellence Cluster of Systems, Neurology, Biomedical Center, 82152 Martinsried-Planegg, Germany.
Magdalena
M
Götz
Instituto de Neurociencias, Consejo Superior de Investigaciones Científicas and Universidad Miguel Hernández, Sant Joan d'Alacant 03550, Spain.
Víctor
V
Borrell
D000818:Animals; D064200:Cdh1 Proteins / Q000235:genetics / Q000378:metabolism*; D019070:Cell Lineage / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D000066673:Cell Self Renewal; D063928:Ependymoglial Cells / Q000378:metabolism*; D005289:Ferrets; D020869:Gene Expression Profiling / Q000379:methods; D018507:Gene Expression Regulation, Developmental; D020547:Lateral Ventricles / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D019579:Neocortex / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D055495:Neurogenesis / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D059008:Time-Lapse Imaging / Q000379:methods
D064200:Cdh1 Proteins; D009687:Nuclear Proteins