A review of methods for nematode identification.
Nematodes are non-segmented roundworms found in soil, aquatic environment, plants, or animals. Either useful or pathogenic, they greatly influence environmental equilibrium, human and animal health, as well as plant production. Knowledge on their taxonomy and biology are key issues to answer the different challenges associated to these organisms. Nowadays, most of the nematode taxonomy remains unknown or unclear. Several approaches are available for parasite identification, from the traditional morphology-based techniques to the sophisticated high-throughput sequencing technologies. All these techniques have advantages or drawbacks depending on the sample origin and the number of nematodes to be processed. This review proposes an overview of all newly available methods available to identify known and/or unknown nematodes with a specific focus on emerging high-throughput molecular techniques.
ANSES, Laboratory for Food Safety, F-62200 Boulogne-sur-mer, France; Univ. Lille, CNRS, INSERM, CHU Lille, Institut Pasteur de Lille, U1019, UMR 8204, CIIL, Centre d'Infection et d'Immunité de Lille, F-59000 Lille, France.
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Seesao
ANSES, Laboratory for Food Safety, F-62200 Boulogne-sur-mer, France. Electronic address: melanie.gay@anses.fr.
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Gay
Gènes Diffusion, F-59500 Douai, France; PEGASE-Biosciences, Institut Pasteur de Lille, F-59000 Lille, France.
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Merlin
Univ. Lille, CNRS, INSERM, CHU Lille, Institut Pasteur de Lille, U1019, UMR 8204, CIIL, Centre d'Infection et d'Immunité de Lille, F-59000 Lille, France.
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Viscogliosi
Univ. Lille, CNRS, INSERM, CHU Lille, Institut Pasteur de Lille, U1019, UMR 8204, CIIL, Centre d'Infection et d'Immunité de Lille, F-59000 Lille, France.
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Aliouat-Denis
Gènes Diffusion, F-59500 Douai, France; PEGASE-Biosciences, Institut Pasteur de Lille, F-59000 Lille, France.
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Audebert
D000818:Animals; D016054:DNA, Protozoan / Q000032:analysis*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D009348:Nematoda / Q000033:anatomy & histology / Q000145:classification* / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010944:Plants / Q000469:parasitology; D017422:Sequence Analysis, DNA / Q000379:methods; D012987:Soil / Q000469:parasitology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D016054:DNA, Protozoan; D012987:Soil
Emerging methods; Nematode identification