The Development and Use of Internal Amplification Controls (IACs) with DNA Profiling Kits for Forensic DNA Analysis.
Biological samples recovered for forensic investigations are often degraded and/or have low amounts of DNA; in addition, in some instances the samples may be contaminated with chemicals that can act as PCR inhibitors. As a consequence this can make interpretation of the results challenging with the possibility of having partial profiles and false negative results. Because of the impact of DNA analysis on forensic investigations, it is important to monitor the process of DNA profiling, in particular the amplification reaction. In this chapter we describe a method for the in-house generation and use of internal amplification controls (IACs) with DNA profiling kits to monitor the success of the PCR proces. In the example we show the use of the SGM Plus® kit. These controls can also be used to aid the interpretation of the DNA profile.
School of Forensic and Investigative Sciences, University of Central Lancashire, Preston, Lancashire, PR1 2HE, UK. nathaliezahra30@gmail.com.
Nathalie
N
Zahra
School of Forensic and Investigative Sciences, University of Central Lancashire, Preston, Lancashire, PR1 2HE, UK.
William
W
Goodwin
D004247:DNA / Q000235:genetics*; D016172:DNA Fingerprinting / Q000379:methods*; D053803:Forensic Genetics / Q000379:methods*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011786:Quality Control
D004247:DNA
AmpFℓSTR® SGM Plus®; DNA profiling; IACs; Internal Amplification Controls; PCR; Quality Control