Comparison of collision-induced dissociation and electron-induced dissociation of phillyrin using FT-ICR MS.
Electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry using collision-induced dissociation (CID) and electron capture dissociation (ECD) at high mass resolution was first applied to investigate the characteristic fragment ions of phillyrin. The CID experimental results demonstrated the elemental composition of fragment ions unambiguously, so a reasonable fragmentation pathway of phillyrin was proposed. The ECD fragmentation mechanism was believed to be fundamentally different from the CID method. ECD could be used not only in the biological field but also as a powerful complement to the structural identification of small molecular compounds. The characteristic fragmentation pathways were helpful in analyzing and interpreting the stability and property of the parent ion.
Shandong Academy of Pharmaceutical Sciences, 250001, PR China.
Zhenguang
Z
Lin
Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, PR China. Electronic address: zwlin123@xmu.edu.cn.
Zhiwei
Z
Lin
Institute for Drug Control of Jinan City, Jinan 250001, Shandong, PR China.
Yingdi
Y
Mu
Shandong Academy of Pharmaceutical Sciences, 250001, PR China.
Dong
D
Yan
D004583:Electrons; D005583:Fourier Analysis; D005960:Glucosides / Q000737:chemistry*; D007477:Ions / Q000737:chemistry; D010545:Peroxides / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D005960:Glucosides; D007477:Ions; D010545:Peroxides; C075528:phillyrin
CID; ECD; FT-ICR MS; MS/MS; Phillyrin