Viral Inhibition Mechanism Mediated by Surface-Modified Silica Nanoparticles.
Vaccines and therapies are not available for several diseases caused by viruses, thus viral infections result in morbidity and mortality of millions of people every year. Nanoparticles are considered to be potentially effective in inhibiting viral infections. However, critical issues related to their use include their toxicity and their mechanisms of antiviral action, which are not yet completely elucidated. To tackle these problems, we synthesized silica nanoparticles with distinct surface properties and evaluated their biocompatibility and antiviral efficacy. We show that nanoparticles exhibited no significant toxicity to mammalian cells, while declines up to 50% in the viral transduction ability of two distinct recombinant viruses were observed. We designed experiments to address the mechanism of antiviral action of our nanoparticles and found that their hydrophobic/hydrophilic characters play a crucial role. Our results reveal that the use of functionalized silica particles is a promising approach for controlling viral infection and offer promising strategies for viral control.
Brazilian Synchrotron Light Laboratory, National Center for Research in Energy and Materials , 13083-970, Campinas, Brazil.|Lehrstuhl für Biomedizinische Physik, Physik-Department & Institut für Medizintechnik, Technische Universität München , 85748, Garching, Germany.
Juliana Martins
JM
de Souza E Silva
Brazilian Biosciences National Laboratory, National Center for Research in Energy and Materials , 13083-970, Campinas, Brazil.|Faculty of Pharmaceutical Sciences and Institute of Biology/Department of Biochemistry and Tissue Biology, University of Campinas , 13083-862, Campinas, Brazil.
Talita Diniz Melo
TD
Hanchuk
Brazilian Synchrotron Light Laboratory, National Center for Research in Energy and Materials , 13083-970, Campinas, Brazil.
Murilo Izidoro
MI
Santos
Brazilian Biosciences National Laboratory, National Center for Research in Energy and Materials , 13083-970, Campinas, Brazil.|Faculty of Pharmaceutical Sciences and Institute of Biology/Department of Biochemistry and Tissue Biology, University of Campinas , 13083-862, Campinas, Brazil.
Jörg
J
Kobarg
Brazilian Biosciences National Laboratory, National Center for Research in Energy and Materials , 13083-970, Campinas, Brazil.
Marcio Chaim
MC
Bajgelman
Brazilian Synchrotron Light Laboratory, National Center for Research in Energy and Materials , 13083-970, Campinas, Brazil.
Mateus Borba
MB
Cardoso
D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology; D001672:Biocompatible Materials / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D057927:Hydrophobic and Hydrophilic Interactions; D053758:Nanoparticles / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry / Q000494:pharmacology*; D013499:Surface Properties; D014780:Viruses / Q000187:drug effects*
D000998:Antiviral Agents; D001672:Biocompatible Materials; D012822:Silicon Dioxide
HIV; VSV-G; antiviral; silica nanoparticles; viral inhibition mechanism; virus