5,7,4'-Trihydroxy-6,8-diprenylisoflavone and lupalbigenin, active components of Derris scandens, induce cell death on breast cancer cell lines.
BACKGROUND
Natural products are a potential source for cancer chemotherapeutic development. This current study was performed to investigate the anti-tumor potential of 5,7,4'-trihydroxy-6,8-diprenylisoflavone (TD) and lupalbigenin (LB), plant flavonoids found in Derris scandens Benth (family: Leguminosae), in cancer and normal cell lines.


METHODS
The human breast cancer cell lines MCF-7, MDA-MB-231 and MDA-MB-468, the human colon cancer cell line SW-620, and the mouse fibroblast cell line L-929 were used to test their anti-cancer activity. Apoptotic cell levels were measured by staining with annexin-V and propidium iodide and Western blot analysis was performed to confirm the apoptotic mechanism.


RESULTS
The results revealed that TD and LB showed specific cytotoxicity against MDA-MB-231 and MCF-7 cells. To elucidate mode of cell death via cytotoxic activities, breast cancer cell lines were treated. TD and LB induced MDA-MB-231 and MCF-7 cells to apoptosis, with the highest number of apoptotic cells at 24 and 72h, respectively. Furthermore, TD and LB inhibited cell cycle progression via up-regulation of p21. Both compounds stimulated apoptosis through down-regulation of bcl-2, up-regulation of bax and releasing of cytochrome C proteins.


CONCLUSIONS
TD and LB have significant anti-cancer effects against human breast cancer cells via cell cycle arrest and the induction of apoptosis through mitochondria signaling pathways, and may be potential anti-cancer agents for the treatment of breast cancer.
Department of Biomedical Sciences, Faculty of Medicine, Prince of Songkla University, Hat Yai 90110, Thailand.
Aman
A
Tedasen
Department of Pharmacognosy and Pharmaceutical Botany, CU-Drug and Health Product Innovation Center, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Suchada
S
Sukrong
Department of Pharmacognosy and Pharmaceutical Botany, CU-Drug and Health Product Innovation Center, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Boonchoo
B
Sritularak
Faculty of Science and Industrial Technology, Prince of Songkla University, Surat Thani Campus, Surat Thani 84000, Thailand.
Theera
T
Srisawat
Department of Biomedical Sciences, Faculty of Medicine, Prince of Songkla University, Hat Yai 90110, Thailand; The Excellence Research Laboratory of Cancer Molecular Biology, Prince of Songkla University, Hat Yai 90110, Thailand. Electronic address: gpotchan@medicine.psu.ac.th.
Potchanapond
P
Graidist
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms / Q000473:pathology*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003110:Colonic Neoplasms / Q000473:pathology; D029866:Derris / Q000737:chemistry*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D020128:Inhibitory Concentration 50; D007529:Isoflavones / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice
C000613813:5,7,4'-trihydroxy-6,8-diprenylisoflavone; D007529:Isoflavones; C000599967:lupalbigenin
5,7,4′-Trihydroxy-6,8-diprenylisoflavone; Anti-cancer activity; Apoptosis; Breast cancer; Lupalbigenin