sesC as a genetic marker for easy identification of Staphylococcus epidermidis from other isolates.
Staphylococcus epidermidis is one of the major concerns with respect to hospital-acquired infections. Therefore, a rapid and easy method to identify at species level S. epidermidis isolates out of a broad range of bacteria is necessary. Based on earlier studies, the sesC gene encoding a S. epidermidis surface protein revealed to be a highly conserved gene in this species. By means of an easy and inexpensive PCR assay, the presence of sesC was checked in 438 clinical staphylococcal isolates. Results showed that sesC is specifically present in all S. epidermidis. In conclusion, the sesC gene can be exploited as a genetic marker in order to distinguish S. epidermidis from other isolates.
KU Leuven, University of Leuven, Department of Microbiology and Immunology, Clinical Bacteriology and Mycology, Herestraat 49, box 819, B-3000 Leuven, Belgium; KU Leuven, University of Leuven, Department of Cellular and Molecular Medicine, Switch Laboratory, Herestraat 49, box 802, B-3000 Leuven, Belgium.
Ladan
L
Khodaparast
KU Leuven, University of Leuven, Department of Microbiology and Immunology, Clinical Bacteriology and Mycology, Herestraat 49, box 819, B-3000 Leuven, Belgium; KU Leuven, University of Leuven, Department of Cellular and Molecular Medicine, Switch Laboratory, Herestraat 49, box 802, B-3000 Leuven, Belgium. Electronic address: laleh.khodaparast@switch.vib-kuleuven.be.
Laleh
L
Khodaparast
KU Leuven, University of Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Molecular Bacteriology, Herestraat 49, box 1037, B-3000 Leuven, Belgium.
Lieve
L
Van Mellaert
KU Leuven, University of Leuven, Department of Microbiology and Immunology, Clinical Bacteriology and Mycology, Herestraat 49, box 819, B-3000 Leuven, Belgium.
Mohammad
M
Shahrooei
KU Leuven, University of Leuven, University Hospitals Leuven, Department of Microbiology and Immunology, Herestraat 49, box 7001, B-3000 Leuven, Belgium; KU Leuven, University of Leuven, University Hospitals Leuven, Department of Microbiology and Immunology, Kapucijnenvoer 33 blok I, box 7001, B-3000 Leuven, Belgium.
Marc
M
Van Ranst
KU Leuven, University of Leuven, Department of Microbiology and Immunology, Clinical Bacteriology and Mycology, Herestraat 49, box 819, B-3000 Leuven, Belgium; KU Leuven, University of Leuven, University Hospitals Leuven, Department of Laboratory Medicine, Herestraat 49, box 7003, B-3000 Leuven, Belgium. Electronic address: johan.vaneldere@uzleuven.be.
Johan
J
Van Eldere
D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D003428:Cross Infection / Q000175:diagnosis* / Q000382:microbiology; D017931:DNA Primers / Q000737:chemistry; D015870:Gene Expression; D005819:Genetic Markers; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013212:Staphylococcus epidermidis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
D001426:Bacterial Proteins; D017931:DNA Primers; D005819:Genetic Markers; D008565:Membrane Proteins
Rapid and easy identification; Staphylococcus epidermidis; sesC