Cell sheet technology for the regeneration of gastrointestinal tissue using a novel gastric perforation rat model.
OBJECTIVE
Anastomotic leakage is a major postoperative complication. While cell sheet technology has been gaining popularity in the clinical field, the utility of cell sheets for containing contaminated enteral perforation remains unclear. We established a new gastric perforation rat model and evaluated the efficacy of the myoblast cell sheet to prevent leakage.


METHODS
To establish a suitable rat model, perforations of various sizes were made in the stomach. We then pasted the sheet onto the perforated stomach in our new model. After the operation, the rats were sacrificed and analyzed macroscopically and histologically. Serum levels of C-related protein and hyaluronic acid in the peritoneal cavity washing lavage were also evaluated.


RESULTS
An incision 5 mm in length was found to be the most suitable for evaluation of the cell sheet performance. In the rats where the cell sheet had been pasted onto the perforation site, we noted less adhesion in the abdominal cavity and a significant reduction in the hyaluronic acid levels in the peritoneal cavity washing lavage.


CONCLUSIONS
We evaluated the efficacy of cell sheets for preventing leakage of enteral contents in a gastric perforation rat model. Myoblast cell sheets were able to markedly reduce the degree of both intra-abdominal adhesion and inflammation.
Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki, 852-8501, Japan.
Shiro
S
Tanaka
Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki, 852-8501, Japan.
Kengo
K
Kanetaka
Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki, 852-8501, Japan.
Mio
M
Fujii
Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki, 852-8501, Japan.
Shinichiro
S
Ito
Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki, 852-8501, Japan.
Yusuke
Y
Sakai
Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki, 852-8501, Japan.
Shinichiro
S
Kobayashi
Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki, 852-8501, Japan.
Kosho
K
Yamanouchi
Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki, 852-8501, Japan.
Fumihiko
F
Fujita
Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki, 852-8501, Japan.
Tamotsu
T
Kuroki
Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki, 852-8501, Japan. sueguchi@nagasaki-u.ac.jp.
Susumu
S
Eguchi
D000818:Animals; D002478:Cells, Cultured; D003584:Cytological Techniques / Q000379:methods*; D004195:Disease Models, Animal; D041981:Gastrointestinal Tract / Q000166:cytology* / Q000502:physiology*; D007416:Intestinal Perforation / Q000473:pathology* / Q000503:physiopathology*; D008297:Male; D032446:Myoblasts / Q000502:physiology*; D017207:Rats, Sprague-Dawley; D012038:Regeneration / Q000502:physiology*; D044968:Regenerative Medicine / Q000379:methods*
Anastomotic leakage; Animal model; Cell sheet technology; Gastrointestinal surgery; Regenerative medicine