Quality assurance of histamine analysis in fresh and canned fish.
Histamine determination is relevant for fish safety, quality and trade. Recently a study by the European Union (EU) compared the Codex and the EU mandated methods for the analysis of histamine and observed that they underestimated and overestimated the results, respectively. To solve this problem, a simple and efficient procedure for the extraction and quantification of histamine by ion-pair HPLC method with post-column derivatization and fluorimetric detection is proposed. It was optimized and validated for the analysis of histamine in fish. The method attended the performance criteria established by Commission Decision 2002/657/CE. The method was also submitted to proficiency testing; uncertainty was calculated; and the stability of solutions and standards was investigated. There was no matrix effect. The LOD, LOQ, CCα and CCβ were fit for the purpose. The method was successfully used in the analyses of freshwater fish and fresh and canned tuna.
LBqA - Laboratório de Bioquímica de Alimentos, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Pres. Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil.
Warlley P
WP
Evangelista
LBqA - Laboratório de Bioquímica de Alimentos, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Pres. Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil.
Tarliane M
TM
Silva
LBqA - Laboratório de Bioquímica de Alimentos, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Pres. Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil.
Letícia R
LR
Guidi
LBqA - Laboratório de Bioquímica de Alimentos, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Pres. Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil.
Patrícia A S
PA
Tette
LBqA - Laboratório de Bioquímica de Alimentos, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Pres. Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil.
Ricardo M D
RM
Byrro
LBqA - Laboratório de Bioquímica de Alimentos, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Pres. Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil.
Paula
P
Santiago-Silva
LBqA - Laboratório de Bioquímica de Alimentos, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Pres. Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil; Laboratório de Controle de Qualidade de Produtos Farmaceuticos e Cosmeticos, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Pres. Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil.
Christian
C
Fernandes
LBqA - Laboratório de Bioquímica de Alimentos, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Pres. Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil. Electronic address: mbeatriz@ufmg.br.
Maria Beatriz A
MB
Gloria
D000818:Animals; D001679:Biogenic Amines / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005399:Fishes; D005470:Fluorometry; D057141:Food, Preserved / Q000032:analysis*; D006632:Histamine / Q000032:analysis*; D011786:Quality Control; D015203:Reproducibility of Results; D017747:Seafood / Q000032:analysis*; D014413:Tuna
D001679:Biogenic Amines; D006632:Histamine
Agmatine (PubChem CID: 2794990); Cadaverine (PubChem CID: 80282); Fish; Histamine (PubChem CID: 5818); Histamine analysis; Matrix effect; Phenylethylamine (PubChem CID: 9075); Proficiency test; Putrescine (PubChem CID: 9532); Serotonin (PubChem CID: 160436); Spermidine (PubChem CID: 9539); Spermine (PubChem CID: 9384); Tryptamine (PubChem CID: 67652); Tyramine (PubChem CID: 66449); Uncertainty