Homologues of CsLOB1 in citrus function as disease susceptibility genes in citrus canker.
The lateral organ boundary domain (LBD) genes encode a group of plant-specific proteins that function as transcription factors in the regulation of plant growth and development. Citrus sinensis lateral organ boundary 1 (CsLOB1) is a member of the LBD family and functions as a disease susceptibility gene in citrus bacterial canker (CBC). Thirty-four LBD members have been identified from the Citrus sinensis genome. We assessed the potential for additional members of LBD genes in citrus to function as surrogates for CsLOB1 in CBC, and compared host gene expression on induction of different LBD genes. Using custom-designed transcription activator-like (TAL) effectors, two members of the same clade as CsLOB1, named CsLOB2 and CsLOB3, were found to be capable of functioning similarly to CsLOB1 in CBC. RNA sequencing and quantitative reverse transcription-polymerase chain reaction analyses revealed a set of cell wall metabolic genes that are associated with CsLOB1, CsLOB2 and CsLOB3 expression and may represent downstream genes involved in CBC.
Department of Plant Pathology, University of Florida, Gainesville, FL, USA 32611.
Junli
J
Zhang
Department of Plant Pathology, University of Florida, Gainesville, FL, USA 32611.
Jose Carlos
JC
Huguet-Tapia
Department of Plant Pathology, University of Florida, Gainesville, FL, USA 32611.
Yang
Y
Hu
Department of Plant Pathology, University of Florida, Gainesville, FL, USA 32611.
Jeffrey
J
Jones
Citrus Research and Education Center/Department of Microbiology and Cell Science, University of Florida, Lake Alfred, FL, USA 33850.
Nian
N
Wang
Department of Plant Pathology, Kansas State University, Manhattan, KS, USA 66506.
Sanzhen
S
Liu
Department of Plant Pathology, University of Florida, Gainesville, FL, USA 32611.
Frank F
FF
White
D032084:Citrus sinensis / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology*; D004198:Disease Susceptibility; D018745:Genome, Plant / Q000235:genetics; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*
D010940:Plant Proteins
citrus bacterial canker; lateral organ boundary domain (LBD) genes; susceptibility (S) genes