Regulation of Glucose Metabolism - A Perspective From Cell Bioprocessing.
Cultured mammalian cells are the main workhorses for producing biologics. The performance of these cell culture processes, in terms of both productivity and product quality attributes, is significantly influenced by cellular metabolism. Glucose is the major carbon source for cellular biosynthesis and energy generation. We summarize here recent advances in our understanding of the regulation of glucose metabolism in cultured cells. The versatility of cells to sustain homeostatic states under widely varying environments is made possible by allosteric regulation of the metabolic network, interplay between the signaling pathways, and transcription factors. Understanding the regulation of metabolism holds the key to altering the metabolic regulatory circuit and implementing direct metabolic control over cell culture processes.
Culture Process Development, Pfizer, Inc., 1 Burtt Road, Andover, MA 01810, USA.
Bhanu Chandra
BC
Mulukutla
Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, MN 55455, USA.
Andrew
A
Yongky
Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, MN 55455, USA.
Tung
T
Le
Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA.
Douglas G
DG
Mashek
Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, MN 55455, USA. Electronic address: wshu@umn.edu.
Wei-Shou
WS
Hu
D000818:Animals; D002468:Cell Physiological Phenomena; D004734:Energy Metabolism / Q000502:physiology*; D025461:Feedback, Physiological / Q000502:physiology; D005786:Gene Expression Regulation / Q000502:physiology*; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D053858:Metabolic Networks and Pathways / Q000502:physiology*; D055442:Metabolome / Q000502:physiology*; D008954:Models, Biological
D005947:Glucose
allosteric regulation; biologics; bioprocess; cell culture; metabolism