Whole transcriptome data analysis of zebrafish mutants affecting muscle development.
Formation of the contractile myofibril of the skeletal muscle is a complex process which when perturbed leads to muscular dystrophy. Herein, we provide a mRNAseq dataset on three different zebrafish mutants affecting muscle organization during embryogenesis. These comprise the myosin folding chaperone unc45b (unc45b-/-), heat shock protein 90aa1.1 (hsp90aa1.1-/-) and the acetylcholine esterase (ache-/-) gene. The transcriptome analysis was performed in duplicate experiments at 72 h post-fertilization (hpf) for all three mutants, with two additional times of development (24 hpf and 48 hpf) for unc45b-/-. A total of 20 samples were analyzed by hierarchical clustering for differential gene expression. The data from this study support the observation made in Etard et al. (2015) [1] (http://dx.doi.org/10.1186/s13059-015-0825-8) that a failure to fold myosin activates a unique transcriptional program in the skeletal muscles that is different from that induced in stressed muscle cells.
Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Campus Nord, PO box, Karlsruhe, Germany.
Olivier
O
Armant
Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Campus Nord, PO box, Karlsruhe, Germany.
Victor
V
Gourain
Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Campus Nord, PO box, Karlsruhe, Germany.
Christelle
C
Etard
Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Campus Nord, PO box, Karlsruhe, Germany.
Uwe
U
Strähle
Development; Genetics; Muscle mutant; Zebrafish; mRNASeq