Melatonin Inhibits CXCL10 and MMP-1 Production in IL-1β-Stimulated Human Periodontal Ligament Cells.
Melatonin is a hormone that is mainly secreted by the pineal gland and exhibits a wide spectrum of activities, including antioxidant functions. Melatonin has been detected in gingival crevicular fluid. However, the role of melatonin in periodontal tissue is still uncertain. The aim of this study was to examine the effects of melatonin on inflammatory mediator expression in human periodontal ligament cells (HPDLC). Interleukin (IL)-1β induced CXC chemokine ligand (CXCL)10, matrix metalloproteinase (MMP)-1, and tissue inhibitors of metalloproteinase (TIMP)-1 production in HPDLC. Melatonin decreased CXCL10 and MMP-1 production and increased TIMP-1 production in IL-1β-stimulated HPDLC. Western blot analysis showed that melatonin inhibited p38 mitogen-activated protein kinase (MAPK) and c-jun N-terminal kinase (JNK) phosphorylation, and IkB-α degradation and phosphorylation in IL-1β-stimulated HPDLC. These results suggest that melatonin might inhibit Th1 cell migration by reducing CXCL10 production. Moreover, melatonin might inhibit soft tissue destruction by decreasing MMP-1 production in periodontal lesions.
Department of Conservative Dentistry, Institute of Biomedical Sciences, Tokushima University Graduate School, School, 3-18-15 Kuramoto-cho, Tokushima, Tokushima, 770-8504, Japan.
Ikuko
I
Hosokawa
Department of Conservative Dentistry, Institute of Biomedical Sciences, Tokushima University Graduate School, School, 3-18-15 Kuramoto-cho, Tokushima, Tokushima, 770-8504, Japan. hosokawa@tokushima-u.ac.jp.
Yoshitaka
Y
Hosokawa
Department of Conservative Dentistry, Institute of Biomedical Sciences, Tokushima University Graduate School, School, 3-18-15 Kuramoto-cho, Tokushima, Tokushima, 770-8504, Japan.
Satoru
S
Shindo
Department of Oral Health Care Promotion, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Tokushima, Japan.
Kazumi
K
Ozaki
Department of Conservative Dentistry, Institute of Biomedical Sciences, Tokushima University Graduate School, School, 3-18-15 Kuramoto-cho, Tokushima, Tokushima, 770-8504, Japan.
Takashi
T
Matsuo
D002478:Cells, Cultured; D054357:Chemokine CXCL10 / Q000096:biosynthesis*; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D053583:Interleukin-1beta / Q000494:pharmacology*; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis*; D008550:Melatonin / Q000494:pharmacology*; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects*; D010766:Phosphorylation / Q000187:drug effects; D011494:Protein Kinases / Q000378:metabolism
C114753:CXCL10 protein, human; D054357:Chemokine CXCL10; D018836:Inflammation Mediators; D053583:Interleukin-1beta; D011494:Protein Kinases; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1; D008550:Melatonin
CXCL10; MMP-1; melatonin; periodontal ligament cells