Analysis of Rapidly Mutating Y Chromosome Short Tandem Repeats (RM Y-STRs).
Rapidly mutating (RM) Y-STRs were recently identified and can help to differentiate between paternally related males in paternity and forensic casework. Normal Y-STRs are often used in casework due to their paternal inheritance, which can help to resolve kinship cases, and identify male components in male/female mixtures; however, more discriminating profiles are obtained if rapidly mutating Y-STRs are used. Previously two or three multiplex PCRs have been used to amplify 13 RM Y-STRs; here, an assay amplifying these 13 markers in a single multiplex PCR is described. Commercially available male control DNA samples have been genotyped during the validation of this assay, thus providing a tool for calibrating genotyping results. It is expected that the assay will provide a niche tool for genotyping casework samples.
School of Forensic and Investigative Sciences, University of Central Lancashire, Preston, UK. shadi@uclan.ac.uk.
Sibte
S
Hadi
D041322:Chromosomes, Human, Y; D004247:DNA / Q000235:genetics; D019075:Electrophoresis, Capillary; D005260:Female; D053803:Forensic Genetics / Q000379:methods*; D005838:Genotype; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods; D009154:Mutation
D004247:DNA
Multiplex PCR; Rapidly mutating Y-STRs; Y-STRs