Optimization of pancreatic lipase inhibitory and antioxidant activities of Ilex paraguariensis by using response surface methodology.
Response surface methodology (RSM) using a Box-Behnken design was used to optimize the extraction conditions for obtaining pancreatic lipase inhibitory and antioxidant principles from Ilex paraguariensis leaves. Three influencing factors: extraction time (min), the liquid-solid ratio, and ethanol concentration (%, v/v) were investigated in the ultrasonic extraction process. Optimization of the extraction conditions to obtain a product with minimum PL activity, maximum antioxidant activity, and maximum yield was performed using RSM by focusing on the three target influencing factors. The optimum conditions were established as the ethanol concentration (54.8 %), liquid-solid ratio (35.4), and extraction time (70.0 min). Under these conditions, the 2,2-diphenyl-1-picrylhydrazyl scavenging activity, PL activity, extraction yield were 59.3 ± 3.5, 35.3 ± 3.0, and 34.4 ± 0.4 %, respectively, similar to the theoretical predicted values of 59.7, 35.2, and 34.3 %, respectively.
College of Pharmacy, Korea University, Sejong, 339-700, Republic of Korea.
Kyung-Eon
KE
Oh
College of Pharmacy, Korea University, Sejong, 339-700, Republic of Korea.
Hyeji
H
Shin
College of Pharmacy, Korea University, Sejong, 339-700, Republic of Korea.
Young Ho
YH
Jeon
College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk, 361-763, Republic of Korea.
Yang Hee
YH
Jo
College of Pharmacy, Chungbuk National University, Cheongju, Chungbuk, 361-763, Republic of Korea.
Mi Kyeong
MK
Lee
Department of Chemistry, Jackson State University, Jackson, MS, 39217, USA.
Ken S
KS
Lee
College of Pharmacy, Keimyung University, Daegu, 704-701, Republic of Korea. bdpark@kmu.ac.kr.
Byoungduck
B
Park
College of Pharmacy, Korea University, Sejong, 339-700, Republic of Korea. kylee11@korea.ac.kr.
Ki Yong
KY
Lee
D000818:Animals; D000975:Antioxidants / Q000032:analysis* / Q000494:pharmacology; D002626:Chemistry, Pharmaceutical / Q000379:methods; D004791:Enzyme Inhibitors / Q000032:analysis* / Q000494:pharmacology; D030019:Ilex paraguariensis; D008049:Lipase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010179:Pancreas / Q000201:enzymology*; D010936:Plant Extracts / Q000032:analysis* / Q000494:pharmacology; D018515:Plant Leaves; D013552:Swine
D000975:Antioxidants; D004791:Enzyme Inhibitors; D010936:Plant Extracts; D008049:Lipase
Antioxidant; Box-Behnken design; Ilex paraguariensis; Optimization; Pancreatic lipase; Response surface methodology