Monitoring the impact of bioaugmentation with a PAH-degrading strain on different soil microbiomes using pyrosequencing.
The effect of bioaugmentation with Sphingobium sp. AM strain on different soils microbiomes, pristine soil (PS), chronically contaminated soil (IPK) and recently contaminated soil (Phe) and their implications in bioremediation efficiency was studied by focusing on the ecology that drives bacterial communities in response to inoculation. AM strain draft genome codifies genes for metabolism of aromatic and aliphatic hydrocarbons. In Phe, the inoculation improved the elimination of phenanthrene during the whole treatment, whereas in IPK no improvement of degradation of any PAH was observed. Through the pyrosequencing analysis, we observed that inoculation managed to increase the richness and diversity in both contaminated microbiomes, therefore, independently of PAH degradation improvement, we observed clues of inoculant establishment, suggesting it may use other resources to survive. On the other hand, the inoculation did not influence the bacterial community of PS. On both contaminated microbiomes, incubation conditions produced a sharp increase on Actinomycetales and Sphingomonadales orders, while inoculation caused a relative decline of Actinomycetales. Inoculation of most diverse microbiomes, PS and Phe, produced a coupled increase of Sphingomonadales, Burkholderiales and Rhizobiales orders, although it may exist a synergy between those genera; our results suggest that this would not be directly related to PAH degradation.
Centro de Investigación y Desarrollo en Fermentaciones Industriales, CINDEFI (UNLP, CCT-La Plata, CONICET), La Plata1900, Buenos Aires, Argentina.
Sabrina
S
Festa
Centro de Investigación y Desarrollo en Fermentaciones Industriales, CINDEFI (UNLP, CCT-La Plata, CONICET), La Plata1900, Buenos Aires, Argentina.
Marianela
M
Macchi
Instituto Nacional de Investigación y Desarrollo Pesquero (INIDEP), Mar del Plata 7600, Argentina.
Federico
F
Cortés
Centro de Investigación y Desarrollo en Fermentaciones Industriales, CINDEFI (UNLP, CCT-La Plata, CONICET), La Plata1900, Buenos Aires, Argentina Comisión de Investigaciones Científicas de la Provincia de Buenos Aires, La Plata 1900, Argentina.
Irma S
IS
Morelli
Centro de Investigación y Desarrollo en Fermentaciones Industriales, CINDEFI (UNLP, CCT-La Plata, CONICET), La Plata1900, Buenos Aires, Argentina bibicoppotelli@gmail.com.
Bibiana M
BM
Coppotelli
D000192:Actinomycetales / Q000254:growth & development / Q000378:metabolism*; D001483:Base Sequence; D001673:Biodegradation, Environmental; D042521:Burkholderiaceae / Q000254:growth & development / Q000378:metabolism*; D004787:Environmental Pollution / Q000032:analysis*; D016680:Genome, Bacterial / Q000235:genetics; D064307:Microbiota; D010616:Phenanthrenes / Q000378:metabolism; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012230:Rhizobiaceae / Q000254:growth & development / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D012987:Soil; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism*; D042301:Sphingomonadaceae / Q000254:growth & development / Q000378:metabolism*
D010616:Phenanthrenes; D011084:Polycyclic Aromatic Hydrocarbons; D012336:RNA, Ribosomal, 16S; D012987:Soil; D012989:Soil Pollutants; C031181:phenanthrene
Sphingobium sp. AM genome; bioaugmentation; community diversity; inoculant establishment; pyrosequencing; soil microbiome