Chemically synthesized glycoconjugates on proteins: effects of multivalency and glycoform in vivo.
The biodistributions and in vivo kinetics of chemically prepared glycoconjugates on proteins are reviewed. Chemical methods can be used to conjugate various mono- and oligosaccharides onto a protein surface. The kinetics and organ-specific accumulation profiles of these glycoconjugates, which are introduced through intravenous injections, have been analyzed using conventional dissection studies as well as non-invasive methods such as single photon emission computed tomography (SPECT), positron emission tomography (PET), and fluorescence imaging. The results suggest that glycan-dependent protein distribution kinetics may be useful for pharmacological and diagnostic applications.
Biofunctional Synthetic Chemistry Laboratory, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan. kotzenori@riken.jp and Biofunctional Chemistry Laboratory, A. Butlerov Institute of Chemistry, Kazan Federal University, 18 Kremlyovskaya street, Kazan 420008, Russia and JST PRESTO, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.
Katsunori
K
Tanaka
D000818:Animals; D006001:Glycoconjugates / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D007700:Kinetics; D009844:Oligosaccharides / Q000737:chemistry; D061848:Optical Imaging; D049268:Positron-Emission Tomography; D011506:Proteins / Q000737:chemistry*; D015899:Tomography, Emission-Computed, Single-Photon
D006001:Glycoconjugates; D009844:Oligosaccharides; D011506:Proteins