A Multi-Step miRNA-mRNA Regulatory Network Construction Approach Identifies Gene Signatures Associated with Endometrioid Endometrial Carcinoma.
We aimed to identify endometrioid endometrial carcinoma (EEC)-related gene signatures using a multi-step miRNA-mRNA regulatory network construction approach. Pathway analysis showed that 61 genes were enriched on many carcinoma-related pathways. Among the 14 highest scoring gene signatures, six genes had been previously shown to be endometrial carcinoma. By qRT-PCR and next generation sequencing, we found that a gene signature (CPEB1) was significantly down-regulated in EEC tissues, which may be caused by hsa-miR-183-5p up-regulation. In addition, our literature surveys suggested that CPEB1 may play an important role in EEC pathogenesis by regulating the EMT/p53 pathway. The miRNA-mRNA network is worthy of further investigation with respect to the regulatory mechanisms of miRNAs in EEC. CPEB1 appeared to be a tumor suppressor in EEC. Our results provided valuable guidance for the functional study at the cellular level, as well as the EEC mouse models.
Department of Pathology, Zhujiang Hospital, Southern Medical University, Guangzhou 510515, China. xionghanzhen@126.com.|Department of Pathology, the Third Affiliated Hospital, Guangzhou Medical University, Guangzhou 510150, China. xionghanzhen@126.com.
Hanzhen
H
Xiong
Department of Pathology, the Third Affiliated Hospital, Guangzhou Medical University, Guangzhou 510150, China. qiulianli12@sina.com.
Qiulian
Q
Li
Department of Pathology, the Third Affiliated Hospital, Guangzhou Medical University, Guangzhou 510150, China. ruichaochen1@sina.com.
Ruichao
R
Chen
Department of Pathology, the Third Affiliated Hospital, Guangzhou Medical University, Guangzhou 510150, China. shaoyanliu2009@sina.com.
Shaoyan
S
Liu
Department of Gynaecology, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou 510150, China. wotooqiwawa@163.com.
Qiongyan
Q
Lin
Department of Pathology, the Third Affiliated Hospital, Guangzhou Medical University, Guangzhou 510150, China. zhongtangxiong2008@163.com.
Zhongtang
Z
Xiong
Department of Pathology, the Third Affiliated Hospital, Guangzhou Medical University, Guangzhou 510150, China. jiangqp2003@126.com.
Qingping
Q
Jiang
Department of Pathology, Zhujiang Hospital, Southern Medical University, Guangzhou 510515, China. linlangguo1@sina.com.
Linlang
L
Guo
CPEB1; EMT; endometrial carcinoma; miRNA targets; p53; protein-protein interaction network