Calibration of the HemoCue point-of-care analyser for determining haemoglobin concentration in a lizard and a fish.
Haemoglobin concentration ([Hb]) is measured for a wide variety of animal studies. The use of point-of-care devices, such as the HemoCue, is becoming increasingly common because of their portability, relative ease of use and low cost. In this study, we aimed to determine whether the [Hb] of blue-tongued skink (Tiliqua nigrolutea) blood can be determined accurately using the HemoCue and whether the HemoCue overestimates the [Hb] of reptile blood in a similar manner to fish blood. Additionally, we aimed to test whether ploidy affected [Hb] determined by the HemoCue using blood from diploid and triploid Atlantic salmon (Salmo salar). The HemoCue Hb 201(+) systematically overestimated [Hb] in both blue-tongued skinks and Atlantic salmon, and there was no difference between calibration equations determined for diploid or triploid salmon. The overestimation was systematic in both species and, as such, [Hb] determined by the HemoCue can be corrected using appropriate calibration equations.
CSIRO Agriculture, Integrated and Sustainable Aquaculture Production, Castray Esplanade, Hobart, TAS 7000, Australia; Institute of Marine and Antarctic Science, University of Tasmania, Castray Esplanade, Hobart, TAS 7000, Australia.
Sarah J
SJ
Andrewartha
Biomedical Sciences, College of Public Health Medical and Veterinary Sciences, James Cook University, Townsville, QLD 4812, Australia.
Suzanne L
SL
Munns
School of Biological Sciences, University of Tasmania, Private Bag 55, Hobart, TAS 7001, Australia.
Ashley
A
Edwards
Diploid; HemoCue; fish; haemoglobin concentration; reptile; triploid