Stochastic sensors designed for assessment of biomarkers specific to obesity.
Two stochastic sensors based on the following oleamides: 1-adamantyloleamide and N,N-dimethyl-N-(2-oleylamidoethyl)amine physically immobilized on graphite paste were designed. The sensors were able to determine simultaneously from the whole blood of Wistar rats three biomarkers specific to obesity: leptin, interleukin-6 (IL-6) and plasminogen activator inhibitor 1 (PAI-1). The whole blood samples were obtained from Wistar rats treated with oleoylethanolamide (OEA), (Z)-N-[(1S)-2-hidroxy-1-(phenylmethyl) ethyl]-9octadecenamide (OLA), and with the aqueous solution of 1% Tween 80 used as solvent for oleamides formulations (control samples). The proposed sensors were very sensitive and reliable for the assay of obesity biomarkers in whole blood of rats.
National Institute for Chemical-Pharmaceutical Research and Development ICCF, Bucharest, Romania; Faculty of Applied Chemistry and Materials Science, Politehnica University of Bucharest, Bucharest, Romania.
Catalina
C
Cioates Negut
Laboratory of Electrochemistry and PATLAB Bucharest, National Institute of Research for Electrochemistry and Condensed Matter, Str. Splaiul Independentei Nr. 202, Bucharest, Romania. Electronic address: ralucavanstaden@gmail.com.
Raluca-Ioana
RI
Stefan-van Staden
Faculty of Applied Chemistry and Materials Science, Politehnica University of Bucharest, Bucharest, Romania.
Eleonora-Mihaela
EM
Ungureanu
Department of Clinical Laboratory and Food Safety, University of Medicine and Pharmacy "Carol Davila", Bucharest, Faculty of Pharmacy, Bucharest, Romania.
Denisa Ioana
DI
Udeanu
D000818:Animals; D015415:Biomarkers / Q000097:blood*; D007202:Indicators and Reagents; D015850:Interleukin-6 / Q000097:blood; D020738:Leptin / Q000097:blood; D009765:Obesity / Q000097:blood*; D017395:Plasminogen Activator Inhibitor 1 / Q000097:blood; D011136:Polysorbates; D051381:Rats; D017208:Rats, Wistar; D013269:Stochastic Processes
D015415:Biomarkers; D007202:Indicators and Reagents; D015850:Interleukin-6; D020738:Leptin; D017395:Plasminogen Activator Inhibitor 1; D011136:Polysorbates
IL-6; Leptin; Oleamides; PAI-1; Stochastic sensors