Difference analysis of the enzymatic hydrolysis performance of acid-catalyzed steam-exploded corn stover before and after washing with water.
The difference in the enzymatic hydrolysis yield of acid-catalyzed steam-exploded corn stover (ASC) before and after washing with water reached approximately 15 % under the same conditions. The reasons for the difference in the yield between ASC and washed ASC (wASC) were determined through the analysis of the composition of ASC prehydrolyzate and sugar concentration of enzymatic hydrolyzate. Salts produced by neutralization (CaSO4, Na2SO4, K2SO4, and (NH4)2SO4), sugars (polysaccharides, oligosaccharides, and monosaccharides), sugar-degradation products (weak acids and furans), and lignin-degradation products (ethyl acetate extracts and nine main lignin-degradation products) were back-added to wASC. Results showed that these products, except furans, exerted negative effect on enzymatic hydrolysis. According to the characteristics of acid-catalyzed steam explosion pretreatment, the five sugar-degradation products' mixture and salts [Na2SO4, (NH4)2SO4] showed minimal negative inhibition effect on enzymatic hydrolysis. By contrast, furans demonstrated a promotion effect. Moreover, soluble sugars, such as 13 g/L xylose (decreased by 6.38 %), 5 g/L cellobiose (5.36 %), 10 g/L glucose (3.67 %), as well as lignin-degradation products, and ethyl acetate extracts (4.87 %), exhibited evident inhibition effect on enzymatic hydrolysis. Therefore, removal of soluble sugars and lignin-degradation products could effectively promote the enzymatic hydrolysis performance.
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, 210037, China. zhujj@njfu.edu.cn.|College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, China. zhujj@njfu.edu.cn.|Jiangsu Key Lab of Biomass-based Green Fuel and Chemicals, Nanjing, 210037, China. zhujj@njfu.edu.cn.
Junjun
J
Zhu
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, 210037, China.|College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, China.
Linli
L
Shi
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, 210037, China.|College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, China.
Lingling
L
Zhang
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, 210037, China.|College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, China.|Jiangsu Key Lab of Biomass-based Green Fuel and Chemicals, Nanjing, 210037, China.
Yong
Y
Xu
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, 210037, China.|College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, China.
Qiang
Q
Yong
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, 210037, China.|College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, China.
Jia
J
Ouyang
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, 210037, China.|College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, China.
Shiyuan
S
Yu
D000143:Acids / Q000737:chemistry; D002384:Catalysis; D002480:Cellulase / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry*; D006868:Hydrolysis; D013227:Steam; D014867:Water / Q000737:chemistry*; D003313:Zea mays / Q000737:chemistry*; D001617:beta-Glucosidase / Q000737:chemistry*
D000143:Acids; D005656:Fungal Proteins; D013227:Steam; D014867:Water; D001617:beta-Glucosidase; D002480:Cellulase
Acid-catalyzed steam-exploded corn stover; Enzymatic hydrolysis; Inhibitor; Lignin-degradation products; Salt