Direct intraperitoneal resuscitation with lidocaine, methylene blue and pentoxiphylline combination does not decreases inflammation after intestinal ischemia-reperfusion injury in rats.
OBJECTIVE
To evaluate the effects of an intraperitoneal solution of methylene blue (MB), lidocaine and pentoxyphylline (PTX) on intestinal ischemic and reperfusion injury.


METHODS
Superior mesenteric artery was isolated and clamped in 36 adult male Sprague Dawley rats. After 60 minutes, clamp was removed and a group received intraperitoneally UNITO solution (PTX 25mg/kg + lidocaine 5mg/kg + MB 2mg/kg), while the other group was treated with warm 0.9% NaCl solution. Rats were euthanized 45 min after drug administration. Lung and bowel were collected for histological evaluation (using Park's score) and determination of myeloperoxidase (MPO) and malondialdehyde (MDA) levels.


RESULTS
Control samples showed lymphoplasmocytic infiltrate and crypt necrosis of villi. MPO and MDA measurements shown no differences between treated and control groups.


CONCLUSIONS
The combination of lidocaine, methylene blue and pentoxyphylline administered intraperitoneally at the studied dose, did not decreased histological lesion scores and biochemical markers levels in intestinal ischemia/reperfusion injury.
Assistant Professor, Department of Veterinary Sciences, University of Turin, Italy. Design of the study; technical procedures; acquisition, interpretation and analysis of data; manuscript preparation and writing., Department of Veterinary Sciences, University of Turin, Italy.
Marco
M
Gandini
DVM, Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Liege, Belgium. Technical procedures; acquisition, interpretation and analysis of data; manuscript preparation and writing., Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Liege, Belgium.
Simona
S
Cerri
MS, PhD, Department of Veterinary Sciences, University of Turin, Italy. Analysis and interpretation of data, critical revision., Department of Veterinary Sciences, University of Turin, Italy.
Paola
P
Pregel
DVM, PhD, Department of Veterinary Sciences, University of Turin, Italy. Manuscript writing, critical revision., Department of Veterinary Sciences, University of Turin, Italy.
Gessica
G
Giusto
MS, PhD, Department of Veterinary Sciences, University of Turin, Italy. Interpretation and analysis of data, manuscript preparation and writing., Department of Veterinary Sciences, University of Turin, Italy.
Cristina
C
Vercelli
Assistant Professor, Department of Veterinary Sciences, University of Turin, Italy. Histopathological examination, critical revision., Department of Veterinary Sciences, University of Turin, Italy.
Selina
S
Iussich
Assistant Professor, Department of Veterinary Sciences, University of Turin, Italy. Histopathological examination, critical revision., Department of Veterinary Sciences, University of Turin, Italy.
Massimiliano
M
Tursi
Full Professor, Department of Veterinary Sciences, University of Turin, Italy. Design of the study, interpretation and analysis of data, manuscript preparation and writing., Department of Veterinary Sciences, University of Turin, Italy.
Anna Maria
AM
Farca
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D004338:Drug Combinations; D004357:Drug Synergism; D007249:Inflammation / Q000188:drug therapy / Q000517:prevention & control; D007263:Infusions, Parenteral; D007422:Intestines / Q000098:blood supply* / Q000201:enzymology; D008012:Lidocaine / Q000008:administration & dosage / Q000627:therapeutic use*; D008168:Lung / Q000098:blood supply / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008751:Methylene Blue / Q000008:administration & dosage / Q000627:therapeutic use*; D023421:Models, Animal; D010431:Pentoxifylline / Q000008:administration & dosage / Q000627:therapeutic use*; D009195:Peroxidase / Q000378:metabolism; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy*; D018709:Statistics, Nonparametric
D000893:Anti-Inflammatory Agents; D004338:Drug Combinations; D008315:Malondialdehyde; D008012:Lidocaine; D009195:Peroxidase; D010431:Pentoxifylline; D008751:Methylene Blue