Comparative Experimental and Computational Study of Monoalkyl Chain Phosphatidylcholine-Containing Thermoresponsive Liposomes.
Liposomes containing lysophospholipids are intensively studied as drug delivery systems that are stable at normal body temperature but exhibit fast release of their drug load at slightly elevated temperatures. In this study, the stability and release properties of dipalmitoylglycerophosphocholine (DPPC)-based liposomes incorporating the commonly used lysophosphatidylocholine (lyso-PC), and a series of monoalkyl chain ether-linked phosphatidylcholine, i.e., the biologically relevant monoalkyl chain platelet activating factor (PAF) and its derivatives lyso-PAF and methyl-PAF, were investigated. To this end a series of PEGylated small unilamellar liposomes with DPPC:monoalkyl lipid compositions of 5% and 10% molar ratio were prepared and compared with regard to stability (37 °C) and release properties at elevated temperatures (38-43 °C). All systems were characterized with respect to size distribution, ζ-potential, and phase transition characteristics. The presence of ether-lipids endows liposomes with superior (∼10% increase) release properties at 5% incorporation compared to lyso-PC, while at 10% molar ratio the formulations do not differ significantly, the release being close to 90%. The findings are supported by atomistic molecular dynamics simulations that suggest a correlation between the enhanced permeability and increased penetration of water molecules within the bilayers with density fluctuations resulting from the increased area-per-lipid and the disorder of the lysolipids alkyl chains.
Institute of Nanoscience and Nanotechology, NCSR ''Demokritos" , 15310 Aghia Paraskevi, Attiki, Greece.
Kleopatra
K
Eleftheriou
Institute of Nanoscience and Nanotechology, NCSR ''Demokritos" , 15310 Aghia Paraskevi, Attiki, Greece.
Zili
Z
Sideratou
Institute of Nuclear & Radiological Sciences and Technology, Energy & Safety, NCSR ''Demokritos" , 15310 Aghia Paraskevi, Attiki, Greece.
Angelos
A
Thanassoulas
Institute of Nanoscience and Nanotechology, NCSR ''Demokritos" , 15310 Aghia Paraskevi, Attiki, Greece.
Athanasios
A
Papakyriakou
Institute of Nanoscience and Nanotechology, NCSR ''Demokritos" , 15310 Aghia Paraskevi, Attiki, Greece.
Dimitris
D
Tsiourvas
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry*; D002152:Calorimetry, Differential Scanning; D000067493:Dynamic Light Scattering; D005452:Fluoresceins / Q000737:chemistry / Q000378:metabolism; D008081:Liposomes / Q000737:chemistry* / Q000378:metabolism; D056004:Molecular Dynamics Simulation; D010539:Permeability; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010972:Platelet Activating Factor / Q000737:chemistry / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry; D013696:Temperature
C519184:1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-methoxy-poly(ethylene glycol 2000); D005452:Fluoresceins; D008081:Liposomes; D010714:Phosphatidylethanolamines; D010972:Platelet Activating Factor; D015060:1,2-Dipalmitoylphosphatidylcholine; C024098:6-carboxyfluorescein; D011092:Polyethylene Glycols