Ethanol Extract of Cissus quadrangularis Enhances Osteoblast Differentiation and Mineralization of Murine Pre-Osteoblastic MC3T3-E1 Cells.
Traditional medicinal literature and previous studies have reported the possible role of Cissus quadrangularis (CQ) as an anti-osteoporotic agent. This study examines the effectiveness of CQ in promoting osteoblast differentiation of the murine pre-osteoblast cell line, MC3T3-E1. Ethanolic extract of CQ (CQ-E) was found to affect growth kinetics of MC3T3-E1 cells in a dosage-dependent manner. High concentrations of CQ-E (more than 10 μg/ml) have particularly adverse effects, while lower concentrations of 0.1 and 1 µg/ml were non-toxic and did not affect cell viability. Notably, cell proliferation was significantly increased at the lower concentrations of CQ-E. CQ-E treatment also augmented osteoblast differentiation, as reflected by a substantial increase in expression of the early osteoblast marker ALP activity, and at later stage, by mineralization of extracellular matrix compared to the control group. These findings suggest dose-dependent effect of CQ-E with lower concentrations exhibiting anabolic and osteogenic properties. J. Cell. Physiol. 232: 540-547, 2017. © 2016 Wiley Periodicals, Inc.
School of Biological Sciences, University of the Punjab, Quaid-i-Azam Campus, Lahore, Pakistan.|Department of Biochemistry, University of Vermont College of Medicine, Burlington, Vemont.
Raazia
R
Tasadduq
Department of Biochemistry, University of Vermont College of Medicine, Burlington, Vemont.
Jonathan
J
Gordon
Department of Zoology, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia.
Khalid A
KA
Al-Ghanim
Department of Biochemistry, University of Vermont College of Medicine, Burlington, Vemont.
Jane B
JB
Lian
Department of Orthopedic Surgery and Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota.
Andre J
AJ
Van Wijnen
Department of Biochemistry, University of Vermont College of Medicine, Burlington, Vemont.
Janet L
JL
Stein
Department of Biochemistry, University of Vermont College of Medicine, Burlington, Vemont.
Gary S
GS
Stein
School of Biological Sciences, University of the Punjab, Quaid-i-Azam Campus, Lahore, Pakistan.|Department of Zoology, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia.
Abdul Rauf
AR
Shakoori
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002113:Calcification, Physiologic / Q000187:drug effects* / Q000235:genetics; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D032422:Cissus / Q000737:chemistry*; D000431:Ethanol / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D007700:Kinetics; D051379:Mice; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*
D010936:Plant Extracts; D000431:Ethanol; D000469:Alkaline Phosphatase