Effects of acute exercise on monocyte subpopulations in metabolic syndrome patients.
Acute exercise induces numerous changes in peripheral blood, e.g. counts of leukocytes. CD16posmonocytes, which play a role in the pathogenesis of arteriosclerosis and the metabolic syndrome (MetS), are among the blood cells with the highest fold increase through exercise. So far no studies have investigated the effect of exercise on the blood cell composition of patients with MetS.



Blood cell counts, a wide panel of laboratory tests, as well as lipid and protein content of monocytes and granulocytes were determined in healthy subjects, persons with metabolic risk and MetS patients before and after one minute of exercise at 400 W. Leukocyte counts increased significantly in all groups with CD14posCD16posmonocytes showing the highest fold-change. In MetS patients the fold increase was smaller. They had a higher resting level of CD14posCD16posmonocytes and a lower basal ratio of CD16neg/CD16posmonocytes. A similar ratio of these cells was induced in control and risk subjects after exercise. However, absolute counts of mobilized pro-inflammatory monocytes did not differ significantly. Furthermore, we detected a decrease in protein content of monocytes in controls, but not in MetS patients.



As strenuous exercise is able to mobilize the same amount of pro-inflammatory monocytes in MetS patients as in healthy persons, the elevated basal level of these cells in MetS patients is likely to be caused by enhanced maturation rather than chronic mobilization. The removal of these monocytes from the endothelium might be part of the beneficial effect of exercise on vascular disease. © 2016 International Clinical Cytometry Society.
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, 93042, Germany.
Ralph
R
Wonner
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, 93042, Germany.
Stefan
S
Wallner
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, 93042, Germany.
Evelyn
E
Orsó
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, 93042, Germany.
Gerd
G
Schmitz
D000328:Adult; D015444:Exercise / Q000502:physiology*; D005260:Female; D006801:Humans; D008297:Male; D024821:Metabolic Syndrome / Q000097:blood* / Q000276:immunology*; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology*
exercise; flow cytometry; metabolic syndrome; monocyte subpopulations; monocytes; protein