The effect of dietary supplementation with rumen-protected methionine alone or in combination with rumen-protected choline and betaine on sheep milk and antioxidant capacity.
This study investigated the effects of dietary inclusion of rumen-protected methionine alone or in combination with rumen-protected choline and betaine on: (i) milk yield, chemical composition and fatty acids (FA) profile and (ii) blood plasma glutathione transferase (GST) activity of periparturient ewes. Furthermore, the oxidative stress indicators for measuring total antioxidant and free radical scavenging activity [ferric reducing ability of plasma (FRAP) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays] were also determined in plasma and milk of ewes. Thus, 45 ewes were divided into three equal groups. Each animal of the control group fed daily with a basal diet. The same diet was offered also in each animal of the other two groups. However, the concentrate fed to M group was supplemented with 2.5 g/kg rumen-protected methionine, while the concentrate fed to MCB group with 5 g/kg of a commercial product which contained a combination of methionine, choline and betaine, all three in rumen-protected form. The results showed that the M diet, compared with the control, increased significantly the ewe's milk fat and the total solids content. Likewise, a tendency for higher milk fat and total solids content in ewes fed the MCB diet was also observed. Both M and MCB diets had not noticeable impact on ewes milk FA profile. Significantly higher FRAP values in the blood plasma of ewes fed the MCB and in the milk of ewes fed with the M diet compared with the control were found. Additionally, significantly higher GST activity in the blood plasma of ewes fed the M diet, compared with the control, was observed. Moreover, a significant increase (by 20%) and a tendency for increase (by 16.72%) in the growth rate of lambs nursing ewes fed with M and MCB diets, respectively, compared to controls, were found.
Department of Nutritional Physiology and Feeding, Agricultural University of Athens, Athens, Greece.
E
E
Tsiplakou
Department of Nutritional Physiology and Feeding, Agricultural University of Athens, Athens, Greece.
A
A
Mavrommatis
Department of Nutritional Physiology and Feeding, Agricultural University of Athens, Athens, Greece.
T
T
Kalogeropoulos
Laboratory of Enzyme Technology, Department of Biotechnology, School of Food, Biotechnology and Development, Agricultural University of Athens, Athens, Greece.
M
M
Chatzikonstantinou
Laboratory of Animal Breeding and Husbandry, Agricultural University of Athens, Athens, Greece.
P
P
Koutsouli
Department of Plant Breeding and Biometry, Agricultural University of Athens, Athens, Greece.
K
K
Sotirakoglou
Laboratory of Enzyme Technology, Department of Biotechnology, School of Food, Biotechnology and Development, Agricultural University of Athens, Athens, Greece.
N
N
Labrou
Department of Nutritional Physiology and Feeding, Agricultural University of Athens, Athens, Greece.
G
G
Zervas
D000821:Animal Feed / Q000032:analysis; D000818:Animals; D000975:Antioxidants / Q000737:chemistry; D001622:Betaine / Q000008:administration & dosage* / Q000494:pharmacology; D002794:Choline / Q000008:administration & dosage* / Q000494:pharmacology; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements; D005227:Fatty Acids / Q000737:chemistry; D005260:Female; D007774:Lactation / Q000187:drug effects; D008715:Methionine / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D008892:Milk / Q000737:chemistry*; D011247:Pregnancy; D012417:Rumen / Q000502:physiology*; D012756:Sheep / Q000097:blood / Q000502:physiology*
D000975:Antioxidants; D005227:Fatty Acids; D001622:Betaine; D008715:Methionine; D002794:Choline
betaine; choline; ewes; glutathione; methionine; milk