The Transcription Factor OsWRKY45 Negatively Modulates the Resistance of Rice to the Brown Planthopper Nilaparvata lugens.
WRKY transcription factors play a central role not only in plant growth and development but also in plant stress responses. However, the role of WRKY transcription factors in herbivore-induced plant defenses and their underlying mechanisms, especially in rice, remains largely unclear. Here, we cloned a rice WRKY gene OsWRKY45, whose expression was induced by mechanical wounding, by infestation of the brown planthopper (BPH, Nilaparvata lugens) and by treatment with jasmonic acid (JA) or salicylic acid (SA). The antisense expression of OsWRKY45 (as-wrky) enhanced BPH-induced levels of H₂O₂ and ethylene, reduced feeding and oviposition preference as well as the survival rate of BPH, and delayed the development of BPH nymphs. Consistently, lower population densities of BPH on as-wrky lines, compared to those on wild-type (WT) plants, were observed in field experiments. On the other hand, as-wrky lines in the field had lower susceptibility to sheath blight (caused by Rhizoctonia solani) but higher susceptibility to rice blast (caused by Magnaporthe oryzae) than did WT plants. These findings suggest that OsWRKY45 plays important but contrasting roles in regulating the resistance of rice to pathogens and herbivores, and attention should be paid if OsWRKY45 is used to develop disease or herbivore-resistant rice.
State Key Laboratory of Rice Biology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China. luckyhfjy@163.com.
Jiayi
J
Huangfu
State Key Laboratory of Rice Biology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China. jiancai.2007@163.com.
Jiancai
J
Li
State Key Laboratory of Rice Biology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China. li-ran@hotmail.com.
Ran
R
Li
State Key Laboratory of Rice Biology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China. hmily890404@126.com.
Meng
M
Ye
State Key Laboratory of Rice Biology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China. kpchen7493@163.com.
Peng
P
Kuai
State Key Laboratory of Rice Biology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China. aimarjia@gmail.com.
Tongfang
T
Zhang
State Key Laboratory of Rice Biology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China. yglou@zju.edu.cn.
Yonggen
Y
Lou
D000818:Animals; D003001:Cloning, Molecular; D003517:Cyclopentanes / Q000009:adverse effects; D060467:Disease Resistance; D018506:Gene Expression Regulation, Plant; D006430:Hemiptera / Q000502:physiology*; D012275:Oryza / Q000235:genetics / Q000469:parasitology*; D054883:Oxylipins / Q000009:adverse effects; D010940:Plant Proteins / Q000235:genetics; D030821:Plants, Genetically Modified; D020156:Salicylic Acid / Q000009:adverse effects; D014157:Transcription Factors / Q000235:genetics*
D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; D014157:Transcription Factors; C011006:jasmonic acid; D020156:Salicylic Acid
H2O2; Nilaparvata lugens; OsWRKY45; WRKY transcription factor; ethylene; herbivore-induced plant defense; rice