Biosynthesis and Hydrolysis of Poly(γ-glutamic acid) from Bacillus subtilis IF03335.
Poly(γ-glutamic acid) (PGA) production in Bacillus subtilis IF03335 was studied. When citric acid as a carbon source was added to a glutamic acid medium containing L-glutamic acid and ammonium sulfate, a large amount of pure PGA was produced. On the other hand, when glucose was added to the glutamic acid medium, a by-product was produced, which seemed to be a polysaccharide. Moreover, the mode of hydrolysis was investigated with PGA in aqueous solutions at 80, 100, and 120°C by monitoring the time-dependent changes in the molecular weights. Hydrolytic degradation of PGA was found to proceed through a random chain scission.
a Mitsubishi Paper Mills Limited, Tsukuba Research Laboratories , Wadai, Tsukuba-shi , Ibaraki 300-42 , Japan.
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Goto
a Mitsubishi Paper Mills Limited, Tsukuba Research Laboratories , Wadai, Tsukuba-shi , Ibaraki 300-42 , Japan.|b Research Institute for Polymers and Textiles , Higashi, Tsukuba-shi , Ibaraki 305 , Japan.
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Kunioka