Dietary pyridoxine potentiates thermal tolerance, heat shock protein and protect against cellular stress of Milkfish (Chanos chanos) under endosulfan-induced stress.
We herein report the protective role of pyridoxine in enhancing thermal tolerance of Milkfish Chanos chanos reared under endosulfan-induced stress. Four isocaloric and isonitrogenous diets were prepared with graded levels of pyridoxine (0, 50, 75 and 100 mg/kg). Two hundred and twenty five fishes were randomly distributed into four treatment groups in triplicate, reared under endosulfan-treated water, which were fed with pyridoxine supplemented diet, while the negative control group was reared without endosulfan-treatment and control fed. The concentration of endosulfan in treated water was maintained at a level of 1/40th of LC50 i.e. 0.52 μg/L. Dietary pyridoxine supplementation had significant (p < 0.01) effect on temperature tolerance viz. CTmax (Critical temperature maxima), LTmax (Lethal temperature maxima), CTmin (Critical temperature minima) and LTmin (Lethal temperature minima) of milkfish. The positive correlation was observed between CT max and LTmax (Y = -1.54 + 15.6x, R(2), 0.943) as well as CTmin and LTmin (Y = -1.44 + 1.021x, R(2), 0.941). At the end of the thermal tolerance study, antioxidative status and HSP 70 were significantly reduced in pyridoxine supplemented groups, whereas brain AChE was significantly (p < 0.01) elevated compared to positive and negative control. It is concluded that CTmax, LTmax, CTmin and LTmin, antioxidative status, neurotransmitter enzyme and HSP 70 strengthened the enhancement of thermal tolerance of Milkfish.
ICAR-National Institute of Abiotic Stress Management, Baramati, Pune 413115, India. Electronic address: neeraj_journal@live.in.
Neeraj
N
Kumar
ICAR-Central Institute of Brackishwater Aquaculture, Chennai 600028, India.
K
K
Ambasankar
ICAR-National Institute of Abiotic Stress Management, Baramati, Pune 413115, India.
K K
KK
Krishnani
ICAR-Central Institute of Brackishwater Aquaculture, Chennai 600028, India.
Prem
P
Kumar
ICAR-Directorate of Coldwater Fisheries Research, Uttarakhand 263136, India.
M S
MS
Akhtar
ICAR-Central Institute of Fisheries Education, Mumbai 400061, India.
Shashi
S
Bhushan
ICAR-National Institute of Abiotic Stress Management, Baramati, Pune 413115, India.
P S
PS
Minhas
D000821:Animal Feed / Q000032:analysis; D000818:Animals; D004032:Diet / Q000662:veterinary*; D019587:Dietary Supplements / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D004726:Endosulfan / Q000633:toxicity*; D005399:Fishes / Q000235:genetics / Q000502:physiology*; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D007306:Insecticides / Q000633:toxicity; D011736:Pyridoxine; D011897:Random Allocation; D000071436:Thermotolerance / Q000187:drug effects*; D014803:Vitamin B Complex; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D006360:Heat-Shock Proteins; D007306:Insecticides; D014874:Water Pollutants, Chemical; D014803:Vitamin B Complex; D011736:Pyridoxine; D004726:Endosulfan
Cellular stress; Chanos chanos; Endosulfan; Pyridoxine; Thermal tolerance