Determination of nucleosides and nucleotides in baby foods by hydrophilic interaction chromatography coupled to tandem mass spectrometry in the presence of hydrophilic ion-pairing reagents.
In this work we propose a rapid and efficient method for the joint determination of nucleosides and nucleotides in dairy and non-dairy baby foods based on hydrophilic interaction chromatography coupled to tandem mass spectrometry in the presence of diethylammonium (DEA) as a hydrophilic ion-pairing reagent (IP-HILIC-MS/MS). Sample treatment of the baby food included dilution with water and centrifugal ultrafiltration (CUF) with an additional washing step that notably improved the global performance of the process. Later dilution of the extract with acetonitrile allowed adequate separation in the HILIC system. With the proposed treatment, we obtained extraction recoveries higher than 80% and, additionally, no matrix effects were observed. The CUF-IP-HILIC-MS/MS method was validated according to the 2002/657/EC decision and was used for the quantification of nucleotides and nucleosides in sixteen samples of commercial baby foods.
Department of Analytical Chemistry, Nutrition and Food Science, University of Salamanca, 37008 Salamanca, Spain. Electronic address: mariamv87@usal.es.
María
M
Mateos-Vivas
Department of Analytical Chemistry, Nutrition and Food Science, University of Salamanca, 37008 Salamanca, Spain. Electronic address: erg@usal.es.
Encarnación
E
Rodríguez-Gonzalo
Department of Analytical Chemistry, Nutrition and Food Science, University of Salamanca, 37008 Salamanca, Spain. Electronic address: hamelin@usal.es.
Javier
J
Domínguez-Álvarez
Department of Analytical Chemistry, Nutrition and Food Science, University of Salamanca, 37008 Salamanca, Spain. Electronic address: dgg@usal.es.
Diego
D
García-Gómez
Department of Analytical Chemistry, Nutrition and Food Science, University of Salamanca, 37008 Salamanca, Spain. Electronic address: rcm@usal.es.
Rita
R
Carabias-Martínez
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007202:Indicators and Reagents / Q000032:analysis / Q000378:metabolism; D007225:Infant Food / Q000032:analysis*; D007231:Infant, Newborn; D009705:Nucleosides / Q000032:analysis* / Q000378:metabolism; D009711:Nucleotides / Q000032:analysis* / Q000378:metabolism; D053719:Tandem Mass Spectrometry / Q000379:methods*
D007202:Indicators and Reagents; D009705:Nucleosides; D009711:Nucleotides
Baby foods; Centrifugal ultrafiltration; Hydrophilic interaction chromatography-tandem mass spectrometry; Hydrophilic ion-pairing; Nucleosides; Nucleotides