Multiplex bioimaging of piRNA molecular pathway-regulated theragnostic effects in a single breast cancer cell using a piRNA molecular beacon.
Recently, PIWI-interacting small non-coding RNAs (piRNAs) have emerged as novel cancer biomarkers candidate because of their high expression level in various cancer types and role in the control of tumor suppressor genes. In this study, a novel breast cancer theragnostics probe based on a single system targeting the piRNA-36026 (piR-36026) molecular pathway was developed using a piR-36026 molecular beacon (MB). The piR-36026 MB successfully visualized endogenous piR-36026 biogenesis, which is highly expressed in MCF7 cells (a human breast cancer cell line), and simultaneously inhibited piR-36026-mediated cancer progression in vitro and in vivo. We discovered two tumor suppressor proteins, SERPINA1 and LRAT, that were directly regulated as endogenous piR-36026 target genes in MCF7 cells. Furthermore, multiplex bioimaging of a single MCF7 cell following treatment with piR-36026 MB clearly visualized the direct molecular interaction of piRNA-36026 with SERPINA1 or LRAT and subsequent molecular therapeutic responses including caspase-3 and PI in the nucleus.
Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung-si, Gangwon-do, 270-701, Republic of Korea.
Youn Jung
YJ
Lee
Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung-si, Gangwon-do, 270-701, Republic of Korea.
Sung Ung
SU
Moon
Department of Surgery, Catholic Kwandong University International St. Mary's Hospital, Incheon Metropolitan City, 404-834, Republic of Korea.
Min Geun
MG
Park
Department of Pathology, Catholic Kwandong University International St. Mary's Hospital, Incheon Metropolitan City, 404-834, Republic of Korea.
Woon Yong
WY
Jung
Department of Surgery, Catholic Kwandong University International St. Mary's Hospital, Incheon Metropolitan City, 404-834, Republic of Korea.
Yong Keun
YK
Park
Department of Surgery, Catholic Kwandong University International St. Mary's Hospital, Incheon Metropolitan City, 404-834, Republic of Korea.
Sung Kyu
SK
Song
Department of Surgery, Catholic Kwandong University International St. Mary's Hospital, Incheon Metropolitan City, 404-834, Republic of Korea.
Je Gyu
JG
Ryu
Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung-si, Gangwon-do, 270-701, Republic of Korea.
Yong Seung
YS
Lee
Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung-si, Gangwon-do, 270-701, Republic of Korea.
Hye Jung
HJ
Heo
Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung-si, Gangwon-do, 270-701, Republic of Korea.
Ha Na
HN
Gu
Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung-si, Gangwon-do, 270-701, Republic of Korea.
Su Jeong
SJ
Cho
Al-Jeraisy DNA Research Chair, Department of Zoology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia; Department of Nucleic Acids Research, Genetic Engineering and Biotechnology Research Institute, City for Scientific Research and Technological Applications, Alexandria, Egypt.
Bahy A
BA
Ali
Al-Jeraisy DNA Research Chair, Department of Zoology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia.
Abdulaziz A
AA
Al-Khedhairy
Department of Surgery, Catholic Kwandong University International St. Mary's Hospital, Incheon Metropolitan City, 404-834, Republic of Korea. Electronic address: iklee68@gmail.com.
Ilkyun
I
Lee
Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangneung-si, Gangwon-do, 270-701, Republic of Korea; Catholic Kwandong University International St. Mary's Hospital, Incheon Metropolitan City, 404-834, Republic of Korea. Electronic address: kimsoonhag@empas.com.
Soonhag
S
Kim
D000217:Acyltransferases / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D001940:Breast / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology; D001943:Breast Neoplasms / Q000000981:diagnostic imaging / Q000235:genetics* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D057809:HEK293 Cells; D006801:Humans; D061986:MCF-7 Cells; D008297:Male; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D015345:Oligonucleotide Probes / Q000032:analysis / Q000235:genetics; D061848:Optical Imaging; D034741:RNA, Small Interfering / Q000032:analysis / Q000235:genetics*; D000515:alpha 1-Antitrypsin / Q000235:genetics
D015345:Oligonucleotide Probes; D034741:RNA, Small Interfering; C043392:SERPINA1 protein, human; D000515:alpha 1-Antitrypsin; D000217:Acyltransferases; C056942:lecithin-retinol acyltransferase
Cancer theragnosis; Molecular beacon; Molecular pathway; Multiplex bioimaging; piRNA-36026