Identification of Lactobacillus brevis using a species-specific AFLP-derived marker.
A simple and specific method for the rapid detection and identification of Lactobacillus brevis was developed. A fAFLP (Fluorescent Amplified Fragment Length Polymorphisms) marker for L. brevis was used to design oligonucleotide primers for a species-specific PCR assay, targeting a 125bp fragment of the gene encoding the aldo/keto reductase of the diketogulonate-reductase family of L. brevis. This assay resulted in 100% inclusivity and exclusivity of assignment of strains to the species L. brevis. The analytical specificity of this assay was successfully tested to identify L. brevis isolates from sourdoughs.
National Research Council of Italy, Institute of Sciences of Food Production (CNR-ISPA), Bari, Italy. Electronic address: vincenzina.fusco@ispa.cnr.it.
Vincenzina
V
Fusco
National Research Council of Italy, Institute of Sciences of Food Production (CNR-ISPA), Bari, Italy.
Grazia Marina
GM
Quero
National Research Council of Italy, Institute of Sciences of Food Production (CNR-ISPA), Bari, Italy.
Daniele
D
Chieffi
Max-Rubner-Institut, Department of Microbiology and Biotechnology, Hermann-Weigmann-Straße 1, 24103 Kiel, Germany.
Charles M A P
CM
Franz
D000449:Aldehyde Reductase / Q000235:genetics; D000074408:Aldo-Keto Reductases; D054458:Amplified Fragment Length Polymorphism Analysis / Q000379:methods*; D001483:Base Sequence; D001939:Bread / Q000382:microbiology; D002611:Cheese / Q000382:microbiology; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics*; D005285:Fermentation; D052196:Levilactobacillus brevis / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D008460:Meat / Q000382:microbiology; D058889:Molecular Typing / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D017931:DNA Primers; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D000074408:Aldo-Keto Reductases; D000449:Aldehyde Reductase
Lactic acid bacteria; Lactobacillus brevis; Microbial food quality; Sourdough; Species identification