Method for Dissecting the Auditory Epithelium (Basilar Papilla) in Developing Chick Embryos.
Chickens are an invaluable model for exploring auditory physiology. Similar to humans, the chicken inner ear is morphologically and functionally close to maturity at the time of hatching. In contrast, chicks can regenerate hearing, an ability lost in all mammals, including humans. The extensive morphological, physiological, behavioral, and pharmacological data available, regarding normal development in the chicken auditory system, has driven the progress of the field. The basilar papilla is an attractive model system to study the developmental mechanisms of hearing. Here, we describe the dissection technique for isolating the basilar papilla in developing chick inner ear. We also provide detailed examples of physiological (patch clamping) experiments using this preparation.
School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton, BN2 4GJ, UK.
Snezana
S
Levic
Department of Physiology and Cell Biology, School of Medicine, University of Nevada Reno, 6795 South Edmond Street, Third Floor, Reno, NV, 89118, USA. enyamoah@gmail.com.
Ebenezer N
EN
Yamoah
D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D004210:Dissection / Q000379:methods*; D009925:Organ of Corti / Q000166:cytology* / Q000254:growth & development*; D018408:Patch-Clamp Techniques; D013048:Specimen Handling
Basilar papilla; Development; Hair cells; Patch clamping