Neuronal Nogo-A in New-born Retinal Ganglion Cells: Implication for the Formation of the Age-related Fiber Order in the Optic Tract.
Nogo-A is highly expressed in oligodendrocytes in the adult central nervous system (CNS). Recently it was found that Nogo-A is also expressed in some neuronal types during development. Here, we examined the expression pattern of Nogo-A in both the retina and optic tract (OT) of mouse embryos from E12 to E15. After perturbation of its function in the OT for 5 hr in the brain slice culture system using a Nogo-A specific antibody or antagonist of its receptor (NEP1-40), the optic nerve fibers and growth cones were traced with DiI. We showed that most Tuj-1 positive new-born neurons at E12 were Nogo-A positive. At E15, retinal neurons reduced the Nogo-A expression. It was worth noting that some projecting axons expressed Nogo-A along the retinofugal pathway. On the basis of their specific locations within the superficial half of the OT and the colocalization with GAP-43 (a marker for the newly born growth cones and axons), we concluded that those Nogo-A positive axons were the newly arrived retinal fibers. Blocking the function of Nogo-A with Nogo-A antibody or NEP1-40 resulted in the shift of DiI labeled axons and growth cones from the superficial half to the whole depth of the OT. These results indicate that Nogo-A in the newly born retinal ganglion cells (RGCs) and their axons are involved in sorting out the newly arrived axons to the subpial region of the OT. Anat Rec, 299:1027-1036, 2016. © 2016 Wiley Periodicals, Inc.
Department of Anatomy and Embryology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, 100191, People's Republic of China.
Dongqiang
D
Su
Department of Anatomy and Embryology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, 100191, People's Republic of China.
Huaicun
H
Liu
School of Biomedical Sciences, Faculty of Medicine, the Chinese University of Hong Kong, Hong Kong SAR, People's Republic of China.
Sun-On
SO
Chan
Department of Anatomy and Embryology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, 100191, People's Republic of China.
Jun
J
Wang
D000818:Animals; D001369:Axons / Q000378:metabolism; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism*; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D009412:Nerve Fibers / Q000378:metabolism*; D000070798:Nogo Proteins / Q000378:metabolism*; D009900:Optic Nerve / Q000166:cytology / Q000378:metabolism*; D065866:Optic Tract / Q000166:cytology / Q000378:metabolism*; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000378:metabolism*; D014795:Visual Pathways / Q000378:metabolism*
D000070798:Nogo Proteins
Nogo-A; RGCs; axon guidance; mouse embryo; optic tract