Cytoprotective Effect of Peptide Sedatin, an Agonist of μ/δ-Opioid Receptors, on Primary Culture of Pulmonary Fibroblasts of Albino Rats under Conditions of Oxidative Stress.
We studied the effects of a synthetic analogue of dermorphin peptide sedatin on DNA synthesis, nucleolar apparatus, and parameters of free radical oxidation in the primary culture of pulmonary fibroblasts under conditions of oxidative stress. Oxidative stress significantly enhanced production of superoxide anion radical in the culture, sufficiently inhibited DNA synthesis in fibroblasts, and reduced the size of cell nuclei and parameters of the nucleolar apparatus. Sedatin prevented accumulation of free radical oxidation products and changes in karyometry parameters induced by oxidative stress. The peptide completely eliminated changes in the parameters of fibroblast nucleolar apparatus and abolished the inhibitory effect of oxidative stress on the number of DNA-synthesizing cells. Pretreatment with non-selective opioid receptor antagonist naloxone hydrochloride partially abolished the effects of sedatin in the primary culture of pulmonary fibroblasts.
The Far-Eastern State Medical University, Khabarovsk, Russia. sazen@mail.ru.
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Sazonova
The Far-Eastern State Medical University, Khabarovsk, Russia.
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Samarina
The Far-Eastern State Medical University, Khabarovsk, Russia.|Khabarovsk Branch of Far-Eastern Research Center of Physiology and Pathology of Breath, Research Institute of Maternity and Childhood Protection, Russian Academy of Medical Sciences, Khabarovsk, Russia.
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Lebed'ko
Medical Esthetics Center Biarritz, Khabarovsk, Russia.
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Maltseva
The Far-Eastern State Medical University, Khabarovsk, Russia.
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Timoshin
D000818:Animals; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism*; D008168:Lung / Q000166:cytology*; D009842:Oligopeptides / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D017465:Receptors, Opioid, delta / Q000819:agonists* / Q000378:metabolism*; D017450:Receptors, Opioid, mu / Q000819:agonists* / Q000378:metabolism*
D009842:Oligopeptides; D017465:Receptors, Opioid, delta; D017450:Receptors, Opioid, mu; C493049:sedatin
DNA synthesis; fibroblasts; opioid peptides; oxidative stress