The therapeutic effect of CD133(+) cells derived from human umbilical cord blood on neonatal mouse hypoxic-ischemic encephalopathy model.
OBJECTIVE
Brain damage at birth can cause lifelong neurodevelopmental deficits. Recently, stem cell therapies have been used in several fields of medicine. We previously reported that CD133(+) cells, endothelial progenitor cells derived from human umbilical cord blood, induce nerve extension in an ex vivo hypoxic-ischemic encephalopathy model. Here, we used an in vivo model to examine the effect of CD133(+) cells in neonatal hypoxic-ischemic encephalopathy.


METHODS
Hypoxic-ischemic brain lesions were induced in neonatal severe combined immunodeficiency mice using the Rice-Vannucci method. CD133(+) cells were administered by intraperitoneal injection 24h after injury.


RESULTS
Immunohistochemical analysis revealed that intraperitoneally transplanted CD133(+) cells migrate towards the brain 48h after injection. Moreover, in CD133(+) cell-treated animals, motor function improved and the brain was protected from the hypoxic-ischemic insult compared with untreated animals.


CONCLUSIONS
Our results suggest that CD133(+) cells derived from human umbilical cord blood have therapeutic potential in neonatal hypoxic-ischemic encephalopathy.
Department of Obstetrics and Gynecology, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan. Electronic address: ykidani@hiroshima-u.ac.jp.
Yukie
Y
Kidani
Department of Neuropathology, Institute of Brain Science, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho, Hirosaki 036-8562, Japan.
Yasuo
Y
Miki
Department of Obstetrics and Gynecology, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan.
Nana
N
Nomimura
Department of Obstetrics and Gynecology, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan.
Shiori
S
Minakawa
Department of Obstetrics and Gynecology, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan.
Norifumi
N
Tanaka
Department of Obstetrics and Gynecology, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan.
Hiroshi
H
Miyoshi
Department of Neuropathology, Institute of Brain Science, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho, Hirosaki 036-8562, Japan.
Koichi
K
Wakabayashi
Department of Obstetrics and Gynecology, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan.
Yoshiki
Y
Kudo
D000071916:AC133 Antigen / Q000097:blood*; D000818:Animals; D002465:Cell Movement; D004195:Disease Models, Animal; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D020925:Hypoxia-Ischemia, Brain / Q000097:blood* / Q000503:physiopathology; D051379:Mice; D009043:Motor Activity
D000071916:AC133 Antigen; C000606023:PROM1 protein, human
Brain damage; CD133; Hypoxic-ischemic encephalopathy; Neonatal mouse