Spectrofluorimetric Determination of Beryllium by Mean Centering of Ratio Spectra.
Trace amounts of beryllium has been determined by spectrofluorimetric method that used morin as fluorimetric reagent. Beryllium gives a highly fluorescent complex with morin. The excitation wavelength of morin and Be-morin complex were 410 and 430. The fluorescence spectra of morin and Be-morin complex were overlaped in excitation wavelength of 430 nm. A method based on mean centering of ratio spectra has been performed to remove the interference caused by morin as it overlaps with the Be-morin spectra. The linear range of beryllium concentration is in 0.2-200 ppb range. The parameters of detection limit and RSD were 0.18 ppb and 4.6 % respectively. This method was used for determination of beryllium in copper-beryllium alloy as a real sample. In determination of Be(II), the interference by Cu(II) was very serious, which was eliminated by adding triethanolamine.
Department of analytical chemistry, faculty of sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
Mahmoud
M
Chamsaz
Department of analytical chemistry, faculty of sciences, Ferdowsi University of Mashhad, Mashhad, Iran. k.samghani@gmail.com.
Kobra
K
Samghani
Department of analytical chemistry, faculty of sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
Mohammad Hossein
MH
Arbab-Zavar
Department of analytical chemistry, faculty of sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
Tahereh
T
Heidari
Beryllium; Mean centering of ratio spectra; Morin; Spectrofluorimetry