Antidepressant-like activity of liposomal formulation containing nimodipine treatment in the tail suspension test, forced swim test and MAOB activity in mice.
Previous studies have shown that intracellular calcium ion dysfunction may be an etiological factor in affective illness. Nimodipine (NMD) is a Ca(2+) channel blocker that has been extensively investigated for therapy of central nervous system (CNS) disorders. In this work, we have evaluated the antidepressant-like activity of nimodipine encapsulated into liposomes (NMD-Lipo) in mice through tail suspension and forced swim assays, as well as MAOB activity. During the tail suspension test, the administration of NMD-Lipo at 0.1, 1 and 10mg/kg was able to promote a reduction in the immobility time of animals greater than the positive control (imipramine). In the forced swim test, the immobility time of mice treated with NMD-Lipo was reduced. This reduction was significantly greater than that found in the animals treated with imipramine and paroxetine. This may suggest that NMD-Lipo provides more antidepressant-like activity than in positive controls. The groups that received a combination of liposomal NMD and antidepressant drugs showed lower immobility time than the groups, which were treated only with imipramine or paroxetine. The mice treated with the combination of NMD-Lipo and reserpine presented an increase in the time of immobility compared with animals treated only with NMD-Lipo. There was a significant decrease in MAOB activity in animals treated with NMD-Lipo compared with untreated animals. The results of the tail suspension test, forced swim test and MAOB activity suggested that the antidepressant activity of NMD-Lipo may be related to an increase in the cerebral monoamine concentrations.
Laboratory of Experimental Neurochemistry Research, Federal University of Piaui, Teresina, PI, Brazil; Immunophatology Keizo-Asami Laboratory, Federal University of Pernambuco, Recife, PE, Brazil.
Lina Clara Gayoso E Almendra Ibiapina
LCGEAI
Moreno
Laboratory of Experimental Neurochemistry Research, Federal University of Piaui, Teresina, PI, Brazil.
Hercília Maria Lins
HML
Rolim
Laboratory of Experimental Neurochemistry Research, Federal University of Piaui, Teresina, PI, Brazil.
Rivelilson Mendes
RM
Freitas
Immunophatology Keizo-Asami Laboratory, Federal University of Pernambuco, Recife, PE, Brazil. Electronic address: linaclaramoreno@gmail.com.
Nereide Stela
NS
Santos-Magalhães
D000818:Animals; D000928:Antidepressive Agents / Q000008:administration & dosage* / Q000494:pharmacology; D018687:Antidepressive Agents, Second-Generation / Q000008:administration & dosage; D000929:Antidepressive Agents, Tricyclic / Q000008:administration & dosage; D001522:Behavior, Animal / Q000187:drug effects; D002121:Calcium Channel Blockers / Q000008:administration & dosage*; D003866:Depressive Disorder / Q000188:drug therapy* / Q000378:metabolism; D019417:Hindlimb Suspension; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D007099:Imipramine / Q000008:administration & dosage; D008081:Liposomes / Q000008:administration & dosage / Q000494:pharmacology; D008297:Male; D051379:Mice; D008995:Monoamine Oxidase / Q000378:metabolism; D009553:Nimodipine / Q000008:administration & dosage* / Q000494:pharmacology; D017374:Paroxetine / Q000008:administration & dosage
D000928:Antidepressive Agents; D018687:Antidepressive Agents, Second-Generation; D000929:Antidepressive Agents, Tricyclic; D002121:Calcium Channel Blockers; D008081:Liposomes; D017374:Paroxetine; D009553:Nimodipine; D008995:Monoamine Oxidase; D007099:Imipramine
Forced swim test; Liposomes; Mice; Monoamine oxidase; Nimodipine; Tail suspension test