Cationic liposomes containing antioxidants reduces pulmonary injury in experimental model of sepsis: Liposomes antioxidants reduces pulmonary damage.
The intracellular redox state of alveolar cells is a determining factor for tolerance to oxidative and pro-inflammatory stresses. This study investigated the effects of intratracheal co-administration of antioxidants encapsulated in liposomes on the lungs of rats subjected to sepsis. For this, male rats subjected to sepsis induced by lipopolysaccharide from Escherichia coli or placebo operation were treated (intratracheally) with antibiotic, 0.9% saline and antioxidants encapsulated or non-encapsulated in liposomes. Experimental model of sepsis by cecal ligation and puncture (CLP) was performed in order to expose the cecum. The cecum was then gently squeezed to extrude a small amount of feces from the perforation site. As an index of oxidative damage, superoxide anions, lipid peroxidation, protein carbonyls, catalase activity, nitrates/nitrites, cell viability and mortality rate were measured. Infected animals treated with antibiotic plus antioxidants encapsulated in liposomes showed reduced levels of superoxide anion (54% or 7.650±1.263 nmol/min/mg protein), lipid peroxidation (33% or 0.117±0.041 nmol/mg protein), protein carbonyl (57% or 0.039 ± 0.022 nmol/mg protein) and mortality rate (3.3%), p value <0.001. This treatment also reduced the level of nitrite/nitrate and increased cell viability (90.7%) of alveolar macrophages. Taken togheter, theses results support that cationic liposomes containing antioxidants should be explored as coadjuvants in the treatment of pulmonary oxidative damage.
Department of Microbiology and Cell Culture, Laboratory of Immunopathology Keizo Asami - LIKA, Av. Prof. Moraes Rego, 1235, Cidade Universitária, CEP: 50780-901, Recife, Pernambuco, Brazil. Electronic address: andremgalvao@hotmail.com.
Andre Martins
AM
Galvão
Department of Microbiology and Cell Culture, Laboratory of Immunopathology Keizo Asami - LIKA, Av. Prof. Moraes Rego, 1235, Cidade Universitária, CEP: 50780-901, Recife, Pernambuco, Brazil.
Júlia Siqueira
JS
Galvão
Graduate Program in Biology Apllied to Health Sciences, Laboratory of Immunopathology Keizo Asami - LIKA, Av. Prof. Moraes Rego, 1235, Cidade Universitária, CEP: 50780-901, Recife, Pernambuco, Brazil.
Marcela Araújo
MA
Pereira
Department of Morphology and Physiology, Federal Rural University of Pernambuco - UFRPE, Av. Rua Dom Manoel de Medeiros, s/n, Dois Irmãos, CEP: 52171-900, Recife, Pernambuco, Brazil.
Pabyton Gonçalves
PG
Cadena
Department of Nanobiotechnology, Laboratory of Immunopathology Keizo Asami - LIKA, Av. Prof. Moraes Rego, 1235, Cidade Universitária, CEP: 50780-901, Recife, Pernambuco, Brazil.
Nereide Stella Santos
NS
Magalhães
Division of Respiratory Therapy, Georgia State University, Atlanta, GA 30302, USA.
James B
JB
Fink
Department of Physiotherapy, Federal University of Pernambuco - UFPE, Av. Prof. Moraes Rego, 1235, Cidade Universitária, CEP: 50780-901, Recife, Pernambuco, Brazil.
Armele Dornelas
AD
de Andrade
Department of Microbiology and Cell Culture, Laboratory of Immunopathology Keizo Asami - LIKA, Av. Prof. Moraes Rego, 1235, Cidade Universitária, CEP: 50780-901, Recife, Pernambuco, Brazil.
Celia Maria Machado Barbosa de
CM
Castro
Department of Pharmacology and Physiology, Federal University of Pernambuco - UFPE, Av. Prof. Moraes Rego, 1235, Cidade Universitária, CEP: 50780-901, Recife, Pernambuco, Brazil.
Maria Bernadete
MB
de Sousa Maia
D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage; D000975:Antioxidants / Q000008:administration & dosage*; D002412:Cations / Q000737:chemistry; D002432:Cecum / Q000293:injuries; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D015227:Lipid Peroxidation / Q000187:drug effects; D008070:Lipopolysaccharides; D008081:Liposomes / Q000737:chemistry; D008168:Lung / Q000187:drug effects* / Q000378:metabolism; D055370:Lung Injury / Q000188:drug therapy* / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008297:Male; D020011:Protective Agents / Q000008:administration & dosage; D050050:Protein Carbonylation / Q000187:drug effects; D011897:Random Allocation; D017208:Rats, Wistar; D018805:Sepsis / Q000188:drug therapy* / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism
D000900:Anti-Bacterial Agents; D000975:Antioxidants; D002412:Cations; D008070:Lipopolysaccharides; D008081:Liposomes; D020011:Protective Agents; D013481:Superoxides
Antioxidant; Liposome; Oxidative stress; Pulmonary injury; Sepsis