Neural stem cells injured by oxidative stress can be rejuvenated by GV1001, a novel peptide, through scavenging free radicals and enhancing survival signals.
Oxidative stress is a well-known pathogenic mechanism of a diverse array of neurological diseases, and thus, numerous studies have attempted to identify antioxidants that prevent neuronal cell death. GV1001 is a 16-amino-acid peptide derived from human telomerase reverse transcriptase (hTERT). Considering that hTERT has a strong antioxidant effect, whether GV1001 also has an antioxidant effect is a question of interest. In the present study, we aimed to investigate the effects of GV1001 against oxidative stress in neural stem cells (NSCs). Primary culture NSCs were treated with different concentrations of GV1001 and/or hydrogen peroxide (H2O2) for various time durations. The H2O2 decreased the viability of the NSCs in a concentration-dependent manner, with 200μM H2O2 significantly decreasing both proliferation and migration. However, treatment with GV1001 rescued the viability, proliferation and migration of H2O2-injured NSCs. Consistently, free radical levels were increased in rat NSCs treated with H2O2, while co-treatment with GV1001 significantly reduced these levels, especially the intracellular levels. In addition, GV1001 restored the expression of survival-related proteins and reduced the expression of death-associated ones in NSCs treated with H2O2. In conclusion, GV1001 has antioxidant and neuroprotective effects in NSCs following treatment with H2O2, which appear to be mediated by scavenging free radicals, increasing survival signals and decreasing death signals.
Department of Neurology, Hanyang University College of Medicine, Seoul, South Korea.
Hyun-Hee
HH
Park
Department of Neurology, Bundang Jesaeng General Hospital, Gyeonggi, South Korea.
Hyun-Jung
HJ
Yu
Department of Neuroscience, KAEL-Gemvax Co., Ltd., Seoul, South Korea.
Sangjae
S
Kim
Department of Neuroscience, KAEL-Gemvax Co., Ltd., Seoul, South Korea.
Gabseok
G
Kim
Department of Translational Medicine, Hanyang University Graduate School of Biomedical Science & Engineering, Seoul, South Korea.
Na-Young
NY
Choi
Department of Translational Medicine, Hanyang University Graduate School of Biomedical Science & Engineering, Seoul, South Korea.
Eun-Hye
EH
Lee
Department of Neurology, Hanyang University College of Medicine, Seoul, South Korea.
Young Joo
YJ
Lee
Department of Chemistry and Research Institute of Natural Sciences, Hanyang University, Seoul, South Korea.
Moon-Young
MY
Yoon
Department of Neurology, Hanyang University College of Medicine, Seoul, South Korea. Electronic address: kylee@hanyang.ac.kr.
Kyu-Yong
KY
Lee
Department of Neurology, Hanyang University College of Medicine, Seoul, South Korea; Department of Translational Medicine, Hanyang University Graduate School of Biomedical Science & Engineering, Seoul, South Korea. Electronic address: ksh213@hanyang.ac.kr.
Seong-Ho
SH
Koh
D000818:Animals; D017304:Annexin A5 / Q000378:metabolism; D001921:Brain / Q000166:cytology; D001973:Bromodeoxyuridine / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004249:DNA Damage / Q000187:drug effects; D004622:Embryo, Mammalian; D006861:Hydrogen Peroxide / Q000633:toxicity; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D058953:Neural Stem Cells / Q000187:drug effects*; D016877:Oxidants; D018384:Oxidative Stress / Q000187:drug effects*; D010446:Peptide Fragments / Q000494:pharmacology*; D051381:Rats; D012844:Sincalide / Q000378:metabolism; D013234:Stem Cells / Q000187:drug effects; D019098:Telomerase / Q000494:pharmacology*
D017304:Annexin A5; D016877:Oxidants; D010446:Peptide Fragments; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; C514987:GV1001 peptide; D019098:Telomerase; D001973:Bromodeoxyuridine; D012844:Sincalide
Antioxidant; GV1001; H(2)O(2); Neural stem cells; Peptide; Vaccine