Investigation of the anti-glioma activity of Oviductus ranae protein hydrolysate.
Oviductus Ranae is the dry oviducts of Rana temporaria chensinensis, and it has been reported to have a range of biological activities. This study aimed to investigate the effects of Oviductus Ranae protein hydrolysate (ORPH) on human glioma C6 cell proliferation and apoptosis in vitro and in vivo. Following in vitro treatment, cell viability and colony formation assays showed that ORPH inhibited C6 cell proliferation. In addition, the results of western blotting also demonstrated that ORPH effectively regulated the expression of the apoptosis related proteins, cleaved caspase-3, Bax and Bcl-2, DNA staining and flow cytometry analysis demonstrated that ORPH significantly promoted apoptosis in this cell line, a finding that was confirmed in vivo using terminal deoxynucleotidyl transferase dUTP nick end labeling. Further investigation demonstrated that ORPH increased apoptosis by modulating the release of inflammatory cytokines and the phosphoinositide 3-kinase (PI3K)/AKT signaling pathway; this was demonstrated using a PI3K/AKT inhibitor (NVP-BEZ235). In summary, the present study suggested that ORPH promoted apoptosis and inhibited glioma cell proliferation by influencing the PI3K/AKT signaling pathway.
Changchun University of Chinese Medicine, China.
Xin
X
Sui
Changchun University of Chinese Medicine, China.
Xiao-Hua
XH
Li
Changchun University of Chinese Medicine, China.
Ming-Hua
MH
Duan
Changchun University of Chinese Medicine, China.
Ai-Ling
AL
Jia
Changchun University of Chinese Medicine, China.
Ye
Y
Wang
Changchun University of Chinese Medicine, China.
Da
D
Liu
Changchun University of Chinese Medicine, China. Electronic address: czdliyiping@163.com.
Yi-Ping
YP
Li
Changchun University of Chinese Medicine, China.
Zhi-Dong
ZD
Qiu
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001932:Brain Neoplasms / Q000201:enzymology / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D005910:Glioma / Q000201:enzymology / Q000473:pathology*; D006801:Humans; D007249:Inflammation / Q000473:pathology; D008419:Materia Medica / Q000494:pharmacology*; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D011492:Protein Hydrolysates / Q000494:pharmacology*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
D016207:Cytokines; D008419:Materia Medica; C539466:Oviductus Ranae; D011492:Protein Hydrolysates; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt
Glioma cells; Oviductus ranae; Oviductus ranae protein hydrolysate; PI3K/AKT