Mineral in skeletal elements of the terrestrial crustacean Porcellio scaber: SRμCT of function related distribution and changes during the moult cycle.
Terrestrial isopods moult first the posterior and then the anterior half of the body, allowing for storage and recycling of CaCO3. We used synchrotron-radiation microtomography to estimate mineral content within skeletal segments in sequential moulting stages of Porcellio scaber. The results suggest that all examined cuticular segments contribute to storage and recycling, however, to varying extents. The mineral within the hepatopancreas after moult suggests an uptake of mineral from the ingested exuviae. The total maximum loss of mineral was 46% for the anterior and 43% for the posterior cuticle. The time course of resorption of mineral and mineralisation of the new cuticle suggests storage and recycling of mineral in the posterior and anterior cuticle. The mineral in the anterior pereiopods decreases by 25% only. P. scaber has long legs and can run fast; therefore, a less mineralised and thus lightweight cuticle in pereiopods likely serves to lower energy consumption during escape behaviour. Differential demineralisation occurs in the head cuticle, in which the cornea of the complex eyes remains completely mineralised. The partes incisivae of the mandibles are mineralised before the old cuticle is demineralised and shed. Probably, this enables the animal to ingest the old exuviae after each half moult.
Central Facility for Electron Microscopy, University of Ulm, Albert-Einstein-Allee 11, 89069, Ulm, Germany. Electronic address: andreas.ziegler@uni-ulm.de.
Andreas
A
Ziegler
Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, Universitätsstrasse 5-7, 45117, Essen, Germany.
Frank
F
Neues
Institute of Physiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 14220, Prague 4, Czech Republic.
Jiří
J
Janáček
Helmholtz-Zentrum Geesthacht, Institute of Materials Research, Max-Planck-Str. 1, 21502, Geesthacht, Germany.
Felix
F
Beckmann
Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, Universitätsstrasse 5-7, 45117, Essen, Germany.
Matthias
M
Epple
D000818:Animals; D002113:Calcification, Physiologic; D002119:Calcium Carbonate / Q000737:chemistry; D003198:Computer Simulation; D003315:Cornea / Q000502:physiology; D006257:Head / Q000502:physiology; D043143:Hepatopancreas / Q000502:physiology*; D007091:Image Processing, Computer-Assisted; D019476:Insect Proteins / Q000502:physiology*; D033321:Isopoda / Q000502:physiology*; D008903:Minerals / Q000737:chemistry*; D018978:Molting; D009055:Mouth / Q000502:physiology*; D017356:Synchrotrons; D055114:X-Ray Microtomography
D019476:Insect Proteins; D008903:Minerals; C047663:cuticle proteins, insects; D002119:Calcium Carbonate
Crustacea; Isopoda; Mineral; Moult; Oniscidea; Synchrotron-radiation microtomography