The AP2-type transcription factors DORNRÖSCHEN and DORNRÖSCHEN-LIKE promote G1/S transition.
The paralogous genes DORNRÖSCHEN (DRN) and DORNRÖSCHEN-LIKE (DRNL) encode AP2-type transcription factors that are expressed and act cell-autonomously in the central stem-cell zone or lateral organ founder cells (LOFCs) in the peripheral zone of the Arabidopsis shoot meristem (SAM), but their molecular contribution is unknown. Here, we show using the Arabidopsis thaliana MERISTEM LAYER 1 promoter that DRN and DRNL share a common function in cell cycle progression and potentially provide local competence for G1-S transitions in the SAM. Analysis of double transgenic DRN::erGFP and DRNL::erCERULEAN promoter fusion lines suggests that the trajectory of this cellular competence starts with DRN activity in the central stem-cell zone and extends locally via DRNL activity into groups of founder cells at the IM or FM periphery. Our data support the scenario that after gene duplication, DRN and DRNL acquired different transcription domains within the shoot meristem, but retained protein function that affects cell cycle progression, either centrally in stem cells or peripherally in primordial founder cells, a finding that is of general relevance for meristem function.
Institute of Developmental Biology, Biocenter Cologne, University of Cologne, Zuelpicher Str. 47b, 50674, Cologne, Germany.
Ingo
I
Seeliger
Institute of Developmental Biology, Biocenter Cologne, University of Cologne, Zuelpicher Str. 47b, 50674, Cologne, Germany.
Anneke
A
Frerichs
Institute of Developmental Biology, Biocenter Cologne, University of Cologne, Zuelpicher Str. 47b, 50674, Cologne, Germany.
Dorothea
D
Glowa
Institute of Developmental Biology, Biocenter Cologne, University of Cologne, Zuelpicher Str. 47b, 50674, Cologne, Germany.|Institute of Zoology, Biocenter Cologne, University of Cologne, Zuelpicher Str. 47b, 50674, Cologne, Germany.
Laura
L
Velo
Institute of Developmental Biology, Biocenter Cologne, University of Cologne, Zuelpicher Str. 47b, 50674, Cologne, Germany.
Petra
P
Comelli
Institute of Developmental Biology, Biocenter Cologne, University of Cologne, Zuelpicher Str. 47b, 50674, Cologne, Germany.
John W
JW
Chandler
Institute of Developmental Biology, Biocenter Cologne, University of Cologne, Zuelpicher Str. 47b, 50674, Cologne, Germany. werr@uni-koeln.de.
Wolfgang
http://orcid.org/0000-0002-6627-1685
W
Werr
D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D049109:Cell Proliferation; D059585:G1 Phase Cell Cycle Checkpoints; D055786:Gene Knockout Techniques; D018398:Homeodomain Proteins / Q000235:genetics*; D018519:Meristem / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D018520:Plant Shoots / Q000166:cytology / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
D029681:Arabidopsis Proteins; D018398:Homeodomain Proteins; D014157:Transcription Factors; C103790:ATML1 protein, Arabidopsis; C450147:ESR1 protein, Arabidopsis; C517039:DRNL protein, Arabidopsis
Arabidopsis; Cell cycle; Lateral organ founder cell; Shoot apical meristem