MCM3: A Novel Proliferation Marker in Oral Squamous Cell Carcinoma.
The present study sought to evaluate and compare the immunoexpression of proteins minichromosome maintenance (MCM) 3 and Ki-67 in oral squamous cell carcinoma (OSCC) to assess the potential of these proteins as markers of cellular proliferation. Twenty-eight cases of OSCC, 9 of tumor-free resection margins (TM), and 4 of non-neoplastic oral mucosa (NNM) were subjected to immunohistochemistry to detect the expression of proteins MCM3 and Ki-67. All OSCCs demonstrated positivity for both proteins. In these tumors, greater MCM3 immunoreactivity was observed in comparison with Ki-67, whereas TMs and NNMs exhibited greater Ki-67 expression compared with MCM3. The immunoexpression of Ki-67 seemed to be influenced by the inflammatory process, particularly in TM and NNM. Our findings indicate that although both MCM3 and Ki-67 represent reliable markers of cellular proliferation in OSCC, as MCM3 expression does not appear to be influenced by external factors, this protein may emerge as a novel marker of cellular proliferation in these types of tumors.
Laboratory of Pathology and Molecular Biology, Oswaldo Cruz Foundation.
Ludmila de F
LF
Valverde
Laboratory of Pathology and Molecular Biology, Oswaldo Cruz Foundation.
Raíza D
RD
de Freitas
School of Medicine of the Federal University of Minas Gerais, Minas Gerais.
Thiago de A
TA
Pereira
AC Camargo Cancer Center, São Paulo, Brazil.
Marina F
MF
de Resende
Aristides Maltez Hospital.
Ivan M G
IMG
Agra
Laboratory of Pathology and Molecular Biology, Oswaldo Cruz Foundation.|Laboratory of Oral Surgical Pathology, School of Dentistry of the Federal University of Bahia.
Jean N
JN
Dos Santos
Laboratory of Pathology and Molecular Biology, Oswaldo Cruz Foundation.|Department of Pathology, School of Medicine of Federal University of Bahia, Bahia.
Mitermayer G
MG
Dos Reis
Laboratory of Pathology and Molecular Biology, Oswaldo Cruz Foundation.|Department of Pathology, School of Medicine of Federal University of Bahia, Bahia.
Caroline B S
CBS
Sales
Laboratory of Pathology and Molecular Biology, Oswaldo Cruz Foundation.|Laboratory of Oral Surgical Pathology, School of Dentistry of the Federal University of Bahia.
Clarissa A
CA
Gurgel Rocha
D014408:Biomarkers, Tumor / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D000072662:Margins of Excision; D064126:Minichromosome Maintenance Complex Component 3 / Q000378:metabolism*; D009062:Mouth Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D011379:Prognosis
D014408:Biomarkers, Tumor; D019394:Ki-67 Antigen; C066470:MCM3 protein, human; D064126:Minichromosome Maintenance Complex Component 3