Flow Cytometry-Based Quantification of HIV-Induced T Cell Chemotactic Response.
The human immunodeficiency virus (HIV) infects blood CD4 T cells through binding to CD4 and the chemokine co-receptor, CXCR4 or CCR5. This viral binding to CXCR4 or CCR5 also triggers the activation of a variety of signaling molecules such as LIMK/cofilin and WAVE2/Arp2/3 to promote actin dynamics, which are necessary for viral nuclear migration and the latent infection of blood resting CD4 T cells. In this chapter, we describe the methods for quantification of HIV-induced actin polymerization and cofilin phosphorylation in human T cells using flow cytometry.
National Center for Biodefense and Infectious Diseases, School of System Biology, George Mason University, 10900 University Blvd., Manassas, VA, 20110, USA. ywu8@gmu.edu.
Yuntao
Y
Wu
D051339:Actin Depolymerizing Factors / Q000378:metabolism; D000199:Actins / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000821:virology; D002460:Cell Line; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D005434:Flow Cytometry; D015658:HIV Infections / Q000276:immunology* / Q000378:metabolism / Q000821:virology*; D015497:HIV-1; D006801:Humans; D016130:Immunophenotyping; D008213:Lymphocyte Activation; D010766:Phosphorylation; D019718:Receptors, CXCR4 / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism / Q000821:virology
D051339:Actin Depolymerizing Factors; D000199:Actins; D019718:Receptors, CXCR4
CD4 T cell; CXCR4; Cofilin; F-actin; HIV-1; Phalloidin; gp120