Silencing FAT10 inhibits metastasis of osteosarcoma.
Metastasis is the main challenge of osteosarcoma treatment. Herein, we first reveal the oncogenic role of FAT10 in metastasis of osteosarcoma. FAT10 was upregulated in osteosarcoma, especially in metastatic osteosarcoma. High level of FAT10 was associated with poorer prognosis of osteosarcoma patients. Moreover, Transwell and Matrigel assays revealed that silencing FAT10 significantly inhibited the invasive and migratory abilities of osteosarcoma cells. Metastasis assay in vivo showed that silencing FAT10 decreased the number of mice with distant metastasis. We also found that FAT10 may act its oncogenic functions through regulating HOXB9. Collectively, the results suggested that FAT10 may be a novel therapeutic target for osteosarcoma patients.
Department of Spine Surgery, Department of Orthopedics, The Fourth Affiliated Hospital, China Medical University, Huanggu, Shenyang, Liaoning 110033, P.R. China.
Chengbin
C
Ma
Department of Joint and Oncological Surgery, Department of Orthopedics, The Fourth Affiliated Hospital, China Medical University, Huanggu, Shenyang, Liaoning 110033, P.R. China.
Zhiyu
Z
Zhang
Department of Joint Surgery, Department of Orthopedics, The Fourth Affiliated Hospital, China Medical University, Huanggu, Shenyang, Liaoning 110033, P.R. China.
Yan
Y
Cui
Department of Traumatology, Department of Orthopedics, The Fourth Affiliated Hospital, China Medical University, Huanggu, Shenyang, Liaoning 110033, P.R. China.
Hongmou
H
Yuan
Department of Spine Surgery, Department of Orthopedics, The Fourth Affiliated Hospital, China Medical University, Huanggu, Shenyang, Liaoning 110033, P.R. China.
Feng
F
Wang
D000328:Adult; D000818:Animals; D045744:Cell Line, Tumor; D005260:Female; D020868:Gene Silencing; D018398:Homeodomain Proteins / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D008297:Male; D051379:Mice; D009362:Neoplasm Metastasis; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology; D014452:Ubiquitins / Q000037:antagonists & inhibitors / Q000096:biosynthesis*
C491257:HOXB9 protein, human; D018398:Homeodomain Proteins; C109184:UBD protein, human; D014452:Ubiquitins