Abalone water-soluble matrix for self-healing biomineralization of tooth defects.
Enamel cannot heal by itself if damaged. Hydroxyapatite (HAP) is main component of human enamel. Formation of enamel-like materials for healing enamel defects remains a challenge. In this paper, we successfully isolated the abalone water-soluble matrix (AWSM) with 1.53wt% the abalone water-soluble protein (AWSPro) and 2.04wt% the abalone water-soluble polysaccharide (AWSPs) from abandoned abalone shell, and self-healing biomineralization of tooth defects was successfully achieved in vitro. Based on X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), hot field emission scanning electron microscopy (HFESEM) and energy dispersive spectrometer (EDS) analysis, the results showed that the AWSM can efficiently induce remineralization of HAP. The enamel-like HAP was successfully achieved onto etched enamel's surface due to the presence of the AWSM. Moreover, the remineralized effect of eroded enamel was growing with the increase of the AWSM. This study provides a solution to the resource waste and environmental pollution caused by abandoned abalone shell, and we provides a new method for self-healing remineralization of enamel defects by AWSM and develops a novel dental material for potential clinical dentistry application.
Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China.
Zhenliang
Z
Wen
Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China. Electronic address: ibptcjd@fzu.edu.cn.
Jingdi
J
Chen
The Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou 350002, China.
Hailiang
H
Wang
Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China.
Shengnan
S
Zhong
Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China.
Yimin
Y
Hu
Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China.
Zhili
Z
Wang
Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China; Institute of Biomedical Engineering, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300192, China. Electronic address: zhangqiq@126.com.
Qiqing
Q
Zhang
D000818:Animals; D003743:Dental Enamel / Q000737:chemistry / Q000648:ultrastructure; D006801:Humans; D008974:Mollusca / Q000737:chemistry*; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D014070:Tooth / Q000473:pathology*; D014089:Tooth Remineralization / Q000379:methods*; D014867:Water / Q000737:chemistry*; D014961:X-Ray Diffraction
D014867:Water
Abalone water-soluble matrix; Enamel; Hydroxyapatite; Remineralization; Self-healing