Evaluation of performances of VERSANT HCV RNA 1.0 assay (kPCR) and Roche COBAS AmpliPrep/COBAS TaqMan HCV test v2.0 at low level viremia.
We assess the concordance between low level HCV values obtained using the VERSANT HCV RNA 1.0 Assay (kPCR) and COBAS AmpliPrep/COBAS TaqMan HCV Quantitative Test v2.0. The correlation between the values obtained by the two RT-PCR assays for samples with quantifiable HCV RNA levels revealed that viral load measured by kPCR significantly correlated with that of the CAP/CTM (R=0.644, P<0.0001). The results show a good concordance (n=126/144, 87%); discordant results were mainly observed in the assessment of values below the lower limit of detection of the assays. These variations may have an impact on clinical decisions for patients on HCV triple therapy or interferon- free regimens. It is therefore recommended to monitor individual patients with the same test throughout treatment.
Department of Molecular Medicine, Sapienza University of Rome, Italy.
Laura
L
Mazzuti
Department of Public Health and Infectious Diseases, Sapienza University of Rome, Italy.
Maria Antonietta
MA
Lozzi
University Campus Bio-Medico of Rome, Italy.
Elisabetta
E
Riva
Department of Molecular Medicine, Sapienza University of Rome, Italy.
Paola
P
Maida
Department of Molecular Medicine, Sapienza University of Rome, Italy.
Francesca
F
Falasca
Department of Molecular Medicine, Sapienza University of Rome, Italy.
Guido
G
Antonelli
Department of Molecular Medicine, Sapienza University of Rome, Italy.
Ombretta
O
Turriziani
D016174:Hepacivirus / Q000302:isolation & purification*; D006526:Hepatitis C / Q000097:blood / Q000175:diagnosis*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000097:blood* / Q000302:isolation & purification*; D014766:Viremia
D012367:RNA, Viral
COBAS TaqMan HCV; HCV; Real-time PCR; VERSANT HCV RNA; Viral load