Fluorescence in situ hybridization analysis of the hTERC region in acute myeloid leukemia patients.
OBJECTIVE
The telomerase RNA component (hTERC) gene is located at 3q26. Increased hTERC gene expression has been frequently observed and amplification was shown using fluorescence in situ hybridization (FISH) in different cancers. The aim of this study was to determine whether hTERC gene amplification is detectable by FISH in acute myeloid leukemia (AML) cells.


METHODS
FISH and karyotype results at the time of diagnosis of 23 adult AML patients were retrospectively evaluated. Additionally, fixed cells were hybridized with an hTERC region-specific FISH probe to determine gene amplification.


RESULTS
Ten of the 23 patients had a normal karyotype and 6 had an abnormal karyotype. hTERC region amplification was not observed in any of the patients.


CONCLUSIONS
Although it was reported that hTERC gene amplification may partially contribute to increased telomerase expression and activity in leukemic cells, it is not possible to make such a conclusion based on the results of the this study, as hTERC amplification was not observed in the study group. This suggests that increased telomerase activity via gene amplification in the development of AML may not be as important a factor as it is in solid tumors.
Özge
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Özer
Tuğçe
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Bulakbaşı Balcı
Zerrin
Z
Yılmaz
Department of Medical Genetics, Faculty of Medicine, Başkent University, Kubilay Sok. No: 36 06570 Maltepe, Ankara, Turkey Phone: +90 312 232 44 00-302 E-mail: feridesahin@hotmail.com.
Feride İffet
Fİ
Şahin