Fibroblast growth factor receptor 1 and cytokeratin 20 expressions and their relation to prognostic variables in bladder cancer.
BACKGROUND
Tumor grade and stage are currently the most important prognostic variables in bladder cancer but establishing additional criteria is still needed for effective treatment.


OBJECTIVE
The aim of the study was to assess the expression of fibroblast growth factor receptor 1 (FGFR1) and cytokeratin 20 (CK20) in cancer bladder (CB) and to evaluate their association with the clinicopathological features of the disease.


METHODS
The study included 80 patients diagnosed as bladder cancer of different stages and grades and 80 patients with nonmalignant urothelial diseases of matched age and sex to the malignant group. The expressions of FGFR1 and CK20 in tissue samples were determined by RT-PCR and immunohistochemistry.


RESULTS
The expression levels of FGFR1 and CK20 were increased in the malignant group when compared to the control group (P<0.001 for each). Analysis of their expression showed that levels of FGFR1 and CK20 were significantly higher in invasive tumor stages (pT2-pT4) than in non-invasive stages (pTis, pTa, pT1) (P<0.001). Interestingly, the sensitivity and specificity of combined detection with CK20 and FGFR1 for the differentiation between invasive and non-invasive stages of bladder cancer reached 97.5% and 92.5%, respectively.


CONCLUSIONS
Our results determined overexpression of both FGFR1 and CK20 in CB specimens. The alterations in the expression of FGFR1 and CK20 were associated with disease stage and grade. Lastly, combined detection of FGFR1 and CK20 had a high predictive prognostic value in differentiating invasive from non-invasive carcinoma.
Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Egypt.
Rehab S
RS
Abdul-Maksoud
Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Egypt. Electronic address: sallyshalaby@hotmail.com.
Sally M
SM
Shalaby
Pathology Department, Faculty of Medicine, Zagazig University, Egypt.
Walid S H
WS
Elsayed
Urology Department, Faculty of Medicine, Zagazig University, Egypt.
Saad
S
Elkady
D000328:Adult; D000368:Aged; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053554:Keratin-20 / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D051496:Receptor, Fibroblast Growth Factor, Type 1 / Q000096:biosynthesis*; D015854:Up-Regulation; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology
D053554:Keratin-20; C496348:FGFR1 protein, human; D051496:Receptor, Fibroblast Growth Factor, Type 1
Bladder cancer; Cytokeratin 20; FGFR1; Immunohistochemical methods; RT-PCR