Application of a nanostructured platform and imprinted sol-gel film for determination of chlorogenic acid in food samples.
Chlorogenic acid (CGA) is a polyphenol derivative that widely exists in higher plants like fruits, vegetables, black teas, and some traditional Chinese medicines. In this work, we have proposed a sensitive and selective electrochemical sensor for detection of CGA. The sensor was based on a glassy carbon electrode (GCE) modified with a functional platform by grafting vinyltrimethoxysilane (VTMS) in multi-walled carbon nanotubes (MWCNTs) and covered by a molecularly imprinted siloxane (MIS) film prepared using the sol-gel process. The VTMS was grafted onto the surface of the MWCNTs via in situ free radical polymerization. The MIS was obtained from the acid-catalyzed hydrolysis/condensation of a solution consisting of tetraethoxysilane (TEOS), phenyltriethoxysilane (PTEOS), (3-aminopropyl)trimethoxysilane (APTMS), and CGA as a template molecule. The modification procedure was evaluated by differential pulse voltammetry (DPV) and scanning electron microscopy (SEM). Under optimized operational conditions, a linear response was obtained covering a concentration ranging from 0.08μmolL(-1) to 500μmolL(-1) with a detection limit (LOD) of 0.032μmolL(-1). The proposed sensor was applied to CGA determination in coffee, tomato, and apple samples with recoveries ranging from 99.3% to 108.6%, showing a promising potential application in food samples. Additionally, the imprinted sensor showed a significantly higher affinity for target CGA than the non-imprinted siloxane (NIS) sensor.
Instituto Nacional de Metrologia, Qualidade e Tecnologia, INMETRO, 25250-020 Duque de Caxias, RJ, Brazil.
Carla M
CM
Ribeiro
Instituto Nacional de Metrologia, Qualidade e Tecnologia, INMETRO, 25250-020 Duque de Caxias, RJ, Brazil.
Eliane M
EM
Miguel
Instituto Federal de Educação, Ciência e Tecnologia de Alagoas, IFAL, 57020-600 Maceió, AL, Brazil.
Jonadab Dos S
JDS
Silva
Instituto Federal de Educação, Ciência e Tecnologia de Alagoas, IFAL, 57020-600 Maceió, AL, Brazil.
Cristian B da
CBD
Silva
Universidade Federal de Alagoas, UFAL, 57072-900 Maceió, AL, Brazil.
Marília O F
MOF
Goulart
Instituto de Química, UNICAMP, C. Postal 6154, 13084-971 Campinas, SP, Brazil.
Lauro T
LT
Kubota
Instituto Nacional de Metrologia, Qualidade e Tecnologia, INMETRO, 25250-020 Duque de Caxias, RJ, Brazil.
Fabiano B
FB
Gonzaga
Instituto Federal de Educação, Ciência e Tecnologia de Alagoas, IFAL, 57020-600 Maceió, AL, Brazil.
Wilney J R
WJR
Santos
Instituto Federal de Educação, Ciência e Tecnologia de Alagoas, IFAL, 57020-600 Maceió, AL, Brazil. Electronic address: phaby.lima@gmail.com.
Phabyanno R
PR
Lima
D000975:Antioxidants / Q000032:analysis*; D002726:Chlorogenic Acid / Q000032:analysis*; D003069:Coffee / Q000737:chemistry; D055664:Electrochemical Techniques / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D057230:Limit of Detection; D018551:Solanum lycopersicum / Q000737:chemistry; D027845:Malus / Q000737:chemistry; D054802:Molecular Imprinting / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D044367:Phase Transition; D012833:Siloxanes / Q000737:chemistry*
D000975:Antioxidants; D003069:Coffee; D037742:Nanotubes, Carbon; D012833:Siloxanes; D002726:Chlorogenic Acid
Chlorogenic acid; MWCNTs; Molecular imprinting siloxane; Sol-gel