Protective antibodies against a sphingomyelinase D from Loxosceles intermedia spider venom elicited in mice with different genetic background.
In the present investigation we used a recombinant LiD1 toxin, named rLiD1his, from Loxosceles intermedia brown spider to elicit specific antibodies in mice carrying different Human Leukocyte Antigens class II (HLAII) {DRB1.0401 (DR4), DQB1.0601 (DQ6) and DQB1.0302 (DQ8)} as well as in BALB/C and C57BL/6 control mice. All mice strains produced high antibody titers against rLiD1his but DR4 mice antibodies (the lower responder mice) were not able to recognize L. intermedia crude venom. The anti-rLiD1his sera, except from DR4 mice, were able to neutralize dermonecrotic, hemorrhagic and edematogenic effects of rLiD1his in naïve rabbits. Overlapping peptides from the amino acid sequence of LiD1 toxin were prepared by SPOT method and differences in LiD1 epitope recognition were observed using different mice anti-rLiD1his sera. The region (160)DKVGHDFSGNDDISDVGK(177) was recognized by transgenic DQ8 and DQ6 mice sera. Other epitopes were recognized by at least two different animals' sera including (10)MGHMVNAIGQIDEFVNLG(27), (37)FDDNANPEYTYHGIP(51), (70)GLRSATTPGNSKYQEKLV(87) and (259)AAYKKKFRVATYDDN(273). Among these epitopes, the epitopes 37-51 and 160-177 have already been shown in previously studies as good candidates to be used alone or combined with other peptides to induce protective immune response against Loxosceles venoms. The results presented here highlight the importance of HLAII in antibody response and recognition of specific B-cell epitopes of rLiD1his spider toxin according to HLAII type and impact in the epitopic vaccine development against this spider.
Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas - ICB, UFMG, Brazil.
Camila Franco Batista
CF
Oliveira
Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas - ICB, UFMG, Brazil.
Andrea
A
Vilela
Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas - ICB, UFMG, Brazil.
Luis Augusto M
LA
Coura
Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas - ICB, UFMG, Brazil.
Fernandes Tenório Gomes
FT
Rodrigues
Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas - ICB, UFMG, Brazil.
Ronaldo Alves Pinto
RA
Nagem
Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas - ICB, UFMG, Brazil.
Carlos
C
Chávez-Olortegui
Departamento de Nutrição, Escola de Enfermagem - EE, UFMG, Brazil.
Tatiani U
TU
Maioli
Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas - ICB, UFMG, Brazil.
Liza F
LF
Felicori
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D018985:Epitopes, B-Lymphocyte / Q000276:immunology*; D000068617:Genetic Background; D007106:Immune Sera / Q000276:immunology; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009500:Neutralization Tests; D010727:Phosphoric Diester Hydrolases / Q000276:immunology*; D011817:Rabbits; D013111:Spider Venoms / Q000201:enzymology*
D000906:Antibodies; D018985:Epitopes, B-Lymphocyte; D007106:Immune Sera; D013111:Spider Venoms; C055161:loxosceles venom; D010727:Phosphoric Diester Hydrolases; C055160:sphingomyelin phosphodiesterase D
Anti-venom; B-cell epitopes; HLAII transgenic mice; Loxosceles; Synthetic peptides; Vaccination