The long and short of non-coding RNAs during post-natal growth and differentiation of skeletal muscles: Focus on lncRNA and miRNAs.
Post-natal growth of skeletal muscle is a dynamic process involving proliferation and fusion of myoblasts with elongating myofibres (hyperplasia of myonuclei) until 3 weeks post-natally in mice, with ongoing differentiation and further increases in myofibre size mostly by hypertrophy until about 12 weeks of age. The expression of mRNAs that control these events are well described, but little is known about the in vivo roles of non-coding RNAs (ncRNAs), including both microRNAs (miRNAs) and the lesser-studied long non-coding RNAs (lncRNAs). We analysed expression patterns for a broad range of lncRNAs (including Neat1, Malat1, Sra, Meg3, LncMyoD and linc-MD1), miRNAs and mRNAs in muscles of normal male C57Bl/6J mice at 2 days and 2, 4, 6 and 12 weeks after birth. These post-natal patterns were compared with expression of these RNAs during classic C2C12 myogenesis and differentiation in tissue culture. This overview of RNAs during post-natal skeletal muscle growth provides a novel focus on ncRNAs during this often overlooked growth period, with many potential applications to normal muscle growth in humans and livestock, and to childhood muscle disorders.
School of Anatomy, Physiology and Human Biology, The University of Western Australia, Western Australia (WA), Australia.
Lauren C
LC
Butchart
School of Anatomy, Physiology and Human Biology, The University of Western Australia, Western Australia (WA), Australia; Harry Perkins Institute of Medical Research, WA, Australia.
Archa
A
Fox
School of Anatomy, Physiology and Human Biology, The University of Western Australia, Western Australia (WA), Australia.
Tea
T
Shavlakadze
School of Anatomy, Physiology and Human Biology, The University of Western Australia, Western Australia (WA), Australia.
Miranda D
MD
Grounds
D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D024510:Muscle Development / Q000235:genetics; D018482:Muscle, Skeletal / Q000166:cytology / Q000254:growth & development*; D032446:Myoblasts / Q000166:cytology; D062085:RNA, Long Noncoding / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics
D035683:MicroRNAs; D062085:RNA, Long Noncoding; D012333:RNA, Messenger
Myogenesis; Non-coding RNAs; Post-natal growth; Skeletal muscle differentiation