Optimization Extraction Process of Polysaccharides from Suillus granulatus and Their Antioxidant and Immunological Activities In vitro.
BACKGROUND
Suillus granulatus is an edible and medicinal fungus in China. S. granulatus polysaccharide (SGP) was considered as the main bioactivity compounds in S. granulatus. Therefore, the extraction of SGP and their antioxidant activities were studied in this work.


METHODS
Fruiting bodies of S. granulatus were purchased from a local market (Fushun, China). Response surface methodology was adopted to optimize the extraction conditions of SGP. The antioxidant and immunological activities in vitro were also assayed.


RESULTS
The extraction of SGP was optimized by a Box-Behnken design. The optimal conditions for the extraction of polysaccharides were as follows: Pre-extraction time, 2 h; extraction temperature, 94°C; ratio of water to raw material, 25; and extraction frequency, 2. Under these conditions, the experimental yield of polysaccharides was 5.38% ±0.15%, which agreed with the predicted yield. The antioxidant assay in vitro showed that SGPs had relatively high scavenging ability for hydroxyl radicals and higher scavenging ability for 1,1-diphenyl-2-picrylhydrazyl radical. However, the scavenging ability of SGPs for superoxide anion radical and reducing power was relatively low. The polysaccharides also significantly increased splenocyte proliferation in vitro.


CONCLUSIONS
SGP possessed good antioxidant and immunological activities in vitro and explored as a novel natural antioxidant or functional food.


CONCLUSIONS
The predictive model of Suillus granulatus polysaccharide (SGP) extraction is adequate for the extraction processSGP possessed a good antioxidant activity in vitroLymphocyte proliferation in vitro was significantly increased by SGPPictorial abstract (in MS Powerpoint Format) is submitted as a separated file in the online submission system. Abbreviation used: SGP: Suillus granulatus polysaccharides, RSM: Response surface methodology, BBD: Box-Behnken design, Vc: Ascorbic acid, DPPH: 1,1-diphenyl-2-picrylhydrazyl, MTT: 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, ConA: Concanavalin A, LPS: lipopolysaccharide, RPMI-1640: Roswell Park Memorial Institute-1640.
Department of Biotechnology, College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University, Fushun, P.R. China.
Feng
F
Zhou
Department of Biotechnology, College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University, Fushun, P.R. China.
Song
S
Yan
Department of Biotechnology, College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University, Fushun, P.R. China.
Shuang
S
Chen
Department of Biotechnology, College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University, Fushun, P.R. China.
Liying
L
Gong
Department of Biotechnology, College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University, Fushun, P.R. China.
Tingting
T
Su
Department of Biotechnology, College of Chemistry, Chemical Engineering and Environmental Engineering, Liaoning Shihua University, Fushun, P.R. China.
Zhanyong
Z
Wang
Antioxidant; Suillus granulates; immunological activity; polysaccharides; response surface methodology