The Rab1 in host cells modulates Brucella intracellular survival and binds to Brucella DnaK protein.
The intracellular pathogen Brucella abortus (B. abortus) survives and replicates inside host cells within the Brucella-containing vacuole, in which membrane contains a small GTPase Rab1. Here, we reported that Rab1 mediates B. abortus intracellular growth. Furthermore, B. abortus DnaK was identified to interact with Rab1 using GST pull-down and mass spectrometry analysis. This interaction was confirmed by co-immunoprecipitation and immunofluorescence. Through DnaK-CyaA fusion protein translocation assay and immunofluorescence confocal microscopy, the B. abortus DnaK was proved to be a virB-dependent translocated substrate.
Central Laboratory, The Second Hospital of Jilin University, Changchun, 130041, China.
Ning
N
Liu
College of Veterinary Medicine, Jilin University, Changchun, 130062, China.
Changjiang
C
Sun
Key Laboratory for Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, 130062, China.
Guimei
G
Cui
Key Laboratory for Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, 130062, China.
Pan
P
Wei
Key Laboratory for Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, 130062, China.
Li
L
Yang
Key Laboratory for Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, 130062, China.
Wanchun
W
Sun
Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health, Shandong University, Qilu Hospital, Jinan City, 250012, Shandong, China.
Shuangxi
S
Wang
Key Laboratory for Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, 130062, China. musics@sina.com.
Lin
L
Wang
Key Laboratory for Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, 130062, China. Qishengpeng@yahoo.com.
Qisheng
Q
Peng
D001426:Bacterial Proteins / Q000378:metabolism / Q000502:physiology*; D002003:Brucella abortus / Q000254:growth & development / Q000472:pathogenicity / Q000502:physiology*; D002470:Cell Survival / Q000502:physiology*; D054884:Host-Pathogen Interactions / Q000502:physiology; D011485:Protein Binding / Q000502:physiology; D021381:Protein Transport / Q000502:physiology; D014617:Vacuoles / Q000382:microbiology; D020761:rab1 GTP-Binding Proteins / Q000502:physiology*
D001426:Bacterial Proteins; D020761:rab1 GTP-Binding Proteins
Brucella; DnaK; Rab1