Identification and typing of the emerging pathogen Candida auris by matrix-assisted laser desorption ionisation time of flight mass spectrometry.
Candida auris is an emerging antifungal resistant yeast species causing nosocomial and invasive infections, emphasising the need of improved diagnostics and epidemiological typing methods. We show that MALDI-TOF VITEK-MS followed by amplified length polymorphisms allows for accurate species identification and subsequent epidemiological characterisation of strains encountered during potential outbreaks.
R&D Microbiology, bioMérieux, La Balme les Grottes, France.
Victoria
V
Girard
R&D Microbiology, bioMérieux, La Balme les Grottes, France.
Sandrine
S
Mailler
R&D Microbiology, bioMérieux, La Balme les Grottes, France.
Marion
M
Chetry
R&D Biomathematics, bioMérieux, Grenoble, France.
Céline
C
Vidal
R&D Microbiology, bioMérieux, La Balme les Grottes, France.
Géraldine
G
Durand
R&D Microbiology, bioMérieux, La Balme les Grottes, France.
Alex
A
van Belkum
Laboratório Especial de Micologia, Escola Paulista de Medicina, UNIFESP, São Paulo, Brazil.
Arnaldo L
AL
Colombo
Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands.
Ferry
http://orcid.org/0000-0002-5622-1916
F
Hagen
Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands.|Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, The Netherlands.
Jacques F
http://orcid.org/0000-0003-3253-6080
JF
Meis
Department of Medical Mycology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India.
Anuradha
A
Chowdhary
D000935:Antifungal Agents / Q000494:pharmacology; D002175:Candida / Q000145:classification* / Q000187:drug effects / Q000302:isolation & purification* / Q000472:pathogenicity; D002177:Candidiasis / Q000175:diagnosis / Q000382:microbiology*; D004196:Disease Outbreaks; D006801:Humans; D008826:Microbial Sensitivity Tests; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D000935:Antifungal Agents
Candida auris; MS VITEK; amplified fragment length polymorphism; matrix-assisted laser desorption ionisation-time of flight mass spectrometry