Flavonoid Glycosides Inhibit Sortase A and Sortase A-Mediated Aggregation of Streptococcus mutans, an Oral Bacterium Responsible for Human Dental Caries.
Three flavonoids were isolated from dried flowers of Sophora japonica using repetitive column chromatography and high-performance liquid chromatography. The flavonoids were identified as rutin (1), quercetin-3'-O-methyl-3-O-α-L-rhamnopyranosyl(1 → 6)-β-D-glucopyranoside (2), and quercetin (3) on the basis of spectroscopic analysis and comparison of values reported in the literature. These compounds inhibited the action of sortase A (SrtA) from Streptococcus mutans, a primary etiologic agent of human dental caries. The onset and magnitude of inhibition of saliva-induced aggregation of S. mutans treated with compound 1 was comparable to that of untreated S. mutans with a deletion of the srtA gene.
Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea.
Woo-Young
WY
Yang
Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 08826, Republic of Korea.
Chang-Kwon
CK
Kim
Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea.
Chan-Hong
CH
Ahn
Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea.
Heegyu
H
Kim
Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 08826, Republic of Korea.
Jongheon
J
Shin
Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea.
Ki-Bong
KB
Oh
D019881:Aminoacyltransferases / Q000037:antagonists & inhibitors*; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors*; D003546:Cysteine Endopeptidases; D003731:Dental Caries / Q000382:microbiology*; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D035264:Flowers / Q000737:chemistry; D006027:Glycosides / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D012463:Saliva / Q000382:microbiology; D029909:Sophora / Q000737:chemistry; D013295:Streptococcus mutans / Q000187:drug effects*
D001426:Bacterial Proteins; D005419:Flavonoids; D006027:Glycosides; D010936:Plant Extracts; D019881:Aminoacyltransferases; C120542:sortase A; D003546:Cysteine Endopeptidases
Sophora japonica; Streptococcus mutans; cell aggregation; flavonoids; sortase A