Gallic acid binding to Spatholobus parviflorus lectin provides insight to its quaternary structure forming.
Therapeutic effects of gallic acid (GA) have already been extensively studied. However, its interaction with lectins has not gained much attention. It is of interest to validate the binding profile of GA with Spatholobus parviflorus seed lectin. A combination of Isothermal Titration Calorimetry (ITC), haemagglutination assay and molecular docking was applied on SPL-GA interaction. ITC results showed four binding sites, stoichiometry, n=4, irrespective of the ratio of SPL:GA taken for titration. Difference among the four binding sites of a single molecule of SPL with regard to GA binding kinetic parameters was consistently varying. Similarly, the glide scores obtained for GA in the four different binding clefts of SPL were also conformed to the ITC. The binding of GA on SPL without affecting its sugar binding property could be considered as a boon for glycobiological research. From the presented studies, it could be proposed that the SPL-GA interactions may facilitate drug delivery by specific targeting/attachment by profiling of cell-surface glycans, followed by controlled release of drugs.
Inter University Centre for Bioscience and Department of Biotechnology and Microbiology, Kannur University, Thalassery Campus, Kannur 670661, India.
Sukumaran
S
Surya
Inter University Centre for Bioscience and Department of Biotechnology and Microbiology, Kannur University, Thalassery Campus, Kannur 670661, India.
Krishnan
K
Geethanandan
Inter University Centre for Bioscience and Department of Biotechnology and Microbiology, Kannur University, Thalassery Campus, Kannur 670661, India.
Chittalakkottu
C
Sadasivan
Inter University Centre for Bioscience and Department of Biotechnology and Microbiology, Kannur University, Thalassery Campus, Kannur 670661, India. Electronic address: mharidasm@rediffmail.com.
Madhathilkovilakathu
M
Haridas
D001665:Binding Sites; D002151:Calorimetry; D002241:Carbohydrates / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D007887:Fabaceae / Q000737:chemistry*; D005707:Gallic Acid / Q000737:chemistry / Q000378:metabolism*; D006384:Hemagglutination; D006801:Humans; D037102:Lectins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D062105:Molecular Docking Simulation; D020836:Protein Structure, Quaternary; D013816:Thermodynamics
D002241:Carbohydrates; D037102:Lectins; D005707:Gallic Acid
Gallic acid; Haemagglutination; ITC; SPL