EF Hand Protein IBA2 Promotes Cell Proliferation in Breast Cancers via Transcriptional Control of Cyclin D1.
EF hand (EFh) domain-containing proteins have been implicated in malignant progression, but their precise functional contributions are uncertain. Here, we report evidence that the EFh protein IBA2 promotes the proliferation of breast cancer cells by facilitating their transit through the G1-S cell-cycle transition. Mechanistic investigations revealed that IBA2 acted at the transcriptional level to promote the expression of the critical cell-cycle regulator cyclin D1. Clinically, we found that levels of IBA2 were significantly upregulated in breast cancer specimens, where its expression correlated positively with histologic grade. Our results suggest a key role for IBA2 in mammary tumorigenesis. Cancer Res; 76(15); 4535-45. ©2016 AACR.
State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China. Department of Cell Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China. Peking University Stem Cell Research Center, China National Center for International Research, Peking University Health Science Center, Beijing, China. yingzhang@ioz.ac.cn lils@sari.ac.cn.
Ying
Y
Zhang
Department of Cell Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China. Peking University Stem Cell Research Center, China National Center for International Research, Peking University Health Science Center, Beijing, China.
Shuling
S
Wang
Department of Cell Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China. Peking University Stem Cell Research Center, China National Center for International Research, Peking University Health Science Center, Beijing, China. SARI Center for Stem Cell and Nanomedicine, Shanghai Advanced Research Institute, University of Chinese Academy of Sciences, Shanghai, China. yingzhang@ioz.ac.cn lils@sari.ac.cn.
Lingsong
L
Li
D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019938:Cyclin D1 / Q000378:metabolism*; D020832:EF Hand Motifs / Q000235:genetics*; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D014162:Transfection; D023041:Xenograft Model Antitumor Assays
D019938:Cyclin D1