Characterization and Optimization of Biosynthesis of Bioactive Secondary Metabolites Produced by Streptomyces sp. 8812.
The nutritional requirements and environmental conditions for a submerged culture of Streptomyces sp. 8812 were determined. Batch and fed-batch Streptomyces sp. 8812 fermentations were conducted to obtain high activity of secondary metabolites. In the study several factors were examined for their influence on the biosynthesis of the active metabolites-7-hydroxy-6-oxo-2,3,4,6-tetrahydroisoquinoline-3-carboxy acid (C10H9NO4) and N-acetyl-3,4-dihydroxy-L-phenylalanine (C11H13NO5): changes in medium composition, pH of production medium, various growth phases of seed culture, amino acid supplementation and addition of anion exchange resin to the submerged culture. Biological activities of secondary metabolites were examined with the use of DD-carboxypeptidase 64-575 and horseradish peroxidase. Streptomyces sp. 8812 mycelium was evaluated under fluorescent microscopy and respiratory activity of the strain was analyzed. Moreover, the enzymatic profiles of the strain with the use of Api ZYM test were analyzed and genetic analysis made. Phylogenetic analysis of Streptomyces sp. 8812 revealed that its closest relative is Streptomyces capoamus JCM 4734 (98%), whereas sequence analysis for 16S rRNA gene using NCBI BLAST algorithm showed 100% homology between these two strains. Biosynthetic processes, mycelium growth and enzyme inhibitory activities of these two strains were also compared.
Aleksandra
A
Rajnisz
Adam
A
Guśpiel
Magdalena
M
Postek
Joanna
J
Ziemska
Anna
A
Laskowska
Daniel
D
Rabczenko
Jolanta
J
Solecka
D001431:Bacteriological Techniques; D002244:Carbon / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D025282:Mycelium; D009584:Nitrogen / Q000378:metabolism; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013302:Streptomyces / Q000235:genetics / Q000378:metabolism*
D004791:Enzyme Inhibitors; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D002244:Carbon; D009584:Nitrogen