Toxicity of long chain fatty acids towards acetate conversion by Methanosaeta concilii and Methanosarcina mazei.
Long-chain fatty acids (LCFA) can inhibit methane production by methanogenic archaea. The effect of oleate and palmitate on pure cultures of Methanosaeta concilii and Methanosarcina mazei was assessed by comparing methane production rates from acetate before and after LCFA addition. For both methanogens, a sharp decrease in methane production (> 50%) was observed at 0.5 mmol L(-1) oleate, and no methane was formed at concentrations higher than 2 mmol L(-1) oleate. Palmitate was less inhibitory than oleate, and M. concilii was more tolerant to palmitate than M. mazei, with 2 mmol L(-1) palmitate causing 11% and 64% methanogenic inhibition respectively. This study indicates that M. concilii and M. mazei tolerate LCFA concentrations similar to those previously described for hydrogenotrophic methanogens. In particular, the robustness of M. concilii might contribute to the observed prevalence of Methanosaeta species in anaerobic bioreactors used to treat LCFA-rich wastewater.
Centre of Biological Engineering, University of Minho, Braga, Portugal.
Sérgio A
SA
Silva
Centre of Biological Engineering, University of Minho, Braga, Portugal.
Andreia F
AF
Salvador
Centre of Biological Engineering, University of Minho, Braga, Portugal.
Ana J
AJ
Cavaleiro
Centre of Biological Engineering, University of Minho, Braga, Portugal.
M Alcina
MA
Pereira
Centre of Biological Engineering, University of Minho, Braga, Portugal.|Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands.
Alfons J M
AJ
Stams
Centre of Biological Engineering, University of Minho, Braga, Portugal.
M Madalena
MM
Alves
Centre of Biological Engineering, University of Minho, Braga, Portugal.|Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands.
Diana Z
DZ
Sousa
D000085:Acetates / Q000378:metabolism*; D000693:Anaerobiosis; D019149:Bioreactors / Q000382:microbiology; D008697:Methane / Q000378:metabolism; D017020:Methanosarcina / Q000187:drug effects* / Q000378:metabolism*; D019640:Methanosarcinales / Q000187:drug effects* / Q000378:metabolism*; D019301:Oleic Acid / Q000633:toxicity*; D010168:Palmitates / Q000633:toxicity*; D062065:Wastewater / Q000382:microbiology
D000085:Acetates; D008697:Methane; D019301:Oleic Acid; D010168:Palmitates; D062065:Wastewater