Miscibility of dl-α-tocopherol β-glucoside in DPPC monolayer at air/water and air/solid interfaces.
The role of newly synthesized tocopherol glycosidic derivative in modifying molecular organization and phase transitions of phospholipid monolayer at the air/water interface has been investigated. Two-component Langmuir films of dl-α-tocopheryl β-D-glucopyranoside (BG) mixed with dipalmitoyl phosphatidylcholine (DPPC) in the whole range of mole fractions were formed at the water surface. An analysis of surface pressure versus mean molecular area (π-A) isotherms and Brewster angle microscope images showed that the presence of BG molecules changes the structure and packing of the DPPC monolayer in a BG concentration dependent manner. BG molecules incorporated into DPPC monolayer inhibit its liquid expanded to liquid condensed phase transition proportionally to the BG concentration. The monolayers were also transferred onto solid substrates and visualized using an atomic force microscope. The results obtained indicate almost complete miscibility of BG and DPPC in the monolayers at surface pressures present in the biological cell membrane (30-35·10(-3) N·m(-1)) for a BG mole fraction as high as 0.3. This makes the monolayer less packed and more disordered, leading to an increased permeability. The results support our previous molecular dynamics simulation data.
Department of Physics and Biophysics, Poznan University of Life Sciences, 60-637 Poznan, Poland.
G
G
Neunert
Faculty of Technical Physics, Poznan University of Technology, 60-965 Poznan, Poland.
J
J
Makowiecki
Faculty of Technical Physics, Poznan University of Technology, 60-965 Poznan, Poland.
E
E
Piosik
Faculty of Technical Physics, Poznan University of Technology, 60-965 Poznan, Poland.
R
R
Hertmanowski
Department of Physics and Biophysics, Poznan University of Life Sciences, 60-637 Poznan, Poland.
K
K
Polewski
Faculty of Technical Physics, Poznan University of Technology, 60-965 Poznan, Poland. Electronic address: tomasz.martynski@put.poznan.pl.
T
T
Martynski
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry*; D044367:Phase Transition; D024502:alpha-Tocopherol / Q000737:chemistry*
D005960:Glucosides; D015060:1,2-Dipalmitoylphosphatidylcholine; D024502:alpha-Tocopherol
Dipalmitoyl phosphatidylcholine (DPPC) monolayers; Langmuir and Langmuir-Blodgett films; Tocopherol glycosidic derivative monolayers; Vitamin E derivatives