Enhanced platelet adhesion in essential thrombocythemia after in vitro activation.
OBJECTIVE
Essential thrombocythemia (ET) is a chronic myeloproliferative disorder characterized by elevated platelet counts and increased risk of thrombosis. Ex vivo data suggest increased platelet reactivity in agreement with the increased thrombosis risk, while in vitro tests often detect decreased platelet activity. The present study aimed to investigate adhesion of ET-platelets in vitro, which is an aspect of platelet function that has been addressed in only a few studies on ET patients.


METHODS
The study included 30 ET patients and 14 healthy controls. Platelet adhesion was measured with a static platelet adhesion assay.


RESULTS
The main finding was that ET-platelets were more readily activated by adhesion-inducing stimuli in vitro than control platelets. This was particularly evident in elderly patients and when using multiple stimuli, such as surfaces of collagen or fibrinogen combined with addition of adenosine 5'-diphosphate or ristocetin. Such multiple stimuli resulted in adhesion above the control mean +2 standard deviations for approximately 50% of the patients.


CONCLUSIONS
The results are in accordance with the concept of increased platelet activity in ET, but opposite to most other in vitro studies. We suggest that the conditions in the adhesion assay might mimic the in vivo situation regarding the presence of chronic platelet activation.
Division of Drug Research/Pharmacology, Department of Medical and Health Sciences, Linköping University, SE-581 85 Linköping, Sweden Phone: +46 10 1038071 Fax: +46 13 149106 E-mail: andreas.eriksson@liu.se.
Andreas C
AC
Eriksson
Kourosh
K
Lotfi
Per A
PA
Whiss