Continuous enzymatic biodiesel production from coconut oil in two-stage packed-bed reactor incorporating an extracting column to remove glycerol formed as by-product.
The transesterification of coconut oil with ethanol catalyzed by Burkholderia cepacia lipase immobilized on polysiloxane-polyvinyl alcohol was performed in a continuous flow. The experimental design consisted of a two-stage packed-bed reactor incorporating a column with cationic resin (Lewatit GF 202) to remove the glycerol formed as by-product and the reactor performance was quantified for three different flow rates corresponding to space-times from 10 to 14 h. The influence of space-time on the ethyl ester (FAEE) concentrations, yields and productivities was determined. The reactor operation was demonstrated for space-time of 14 h attaining FAEE concentrations of 58.5 ± 0.87 wt%, FAEE yields of 97.3 ± 1.9 % and productivities of 41.6  ± 1.0 mgester g medium (-1)  h(-1). Biodiesel purified samples showed average kinematic viscosity values of 5.5 ± 0.3 mm(2) s(-1) that meet the criteria established by the American National Standard ASTM (D6751). The immobilized lipase was found to be stable regarding its morphological and catalytic characteristics, showing half-life time (t 1/2) around 1540 h. The continuous packed-bed reactor connected in series with simultaneous glycerol removal has a great potential to attain high level of transesterification yields, raising biodiesel productivity.
Engineering School of Lorena-University of São Paulo, Lorena, São Paulo, 12602-810, Brazil.
William
W
Costa E Silva
Engineering School of Lorena-University of São Paulo, Lorena, São Paulo, 12602-810, Brazil.
Larissa
L
Freitas
Engineering School of Lorena-University of São Paulo, Lorena, São Paulo, 12602-810, Brazil.
Pedro C
PC
Oliveira
Engineering School of Lorena-University of São Paulo, Lorena, São Paulo, 12602-810, Brazil. heizir@dequi.eel.usp.br.
Heizir F
HF
de Castro
D056804:Biofuels; D019149:Bioreactors; D016956:Burkholderia cepacia / Q000254:growth & development*; D000074263:Coconut Oil; D005990:Glycerol / Q000378:metabolism*; D010938:Plant Oils / Q000378:metabolism*
D056804:Biofuels; D010938:Plant Oils; D005990:Glycerol; D000074263:Coconut Oil
Biodiesel; Coconut oil; Glycerol removal; Lipase; Packed-bed reactor