CRISPR/Cas9 genome editing throws descriptive 3-D genome folding studies for a loop.
CRISPR/Cas9 genome editing studies have recently shed new light into the causal link between the linear DNA sequence and 3-D chromatin architecture. Here we describe current models for the folding of genomes into a nested hierarchy of higher-order structures and discuss new insights into the organizing principles governing genome folding at each length scale. WIREs Syst Biol Med 2016, 8:286-299. doi: 10.1002/wsbm.1338 For further resources related to this article, please visit the WIREs website.
Department of Bioengineering, University of Pennsylvania, Philadelphia, PA, USA.
Jonathan A
JA
Beagan
Department of Bioengineering, University of Pennsylvania, Philadelphia, PA, USA.|Epigenetics Program, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Jennifer E
JE
Phillips-Cremins
D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics / Q000378:metabolism; D000076246:CCCTC-Binding Factor; D064113:CRISPR-Cas Systems / Q000235:genetics*; D002843:Chromatin / Q000737:chemistry / Q000378:metabolism; D002875:Chromosomes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000072669:Gene Editing / Q000379:methods*; D016678:Genome; D006801:Humans; D009690:Nucleic Acid Conformation; D012097:Repressor Proteins / Q000378:metabolism
D000076246:CCCTC-Binding Factor; C000619697:CTCF protein, human; D002843:Chromatin; D012097:Repressor Proteins