Effects of a Hot-Water Extract of Allium hookeri Roots on Bone Formation in Human Osteoblast-Like MG-63 Cells In Vitro and in Rats In Vivo.
Allium hookeriis a wild herb found mainly in the Himalayas, growing at altitudes of 1400-4200 m.A. hookeriis widely consumed as a vegetable and herbal medicine in Asia, but its effects on bone health have not been reported previously. This study investigated the effects of a hot-water extract ofA. hookeriroots on bone formation. The hot-water extract significantly increased the proliferation ofin vitrohuman osteoblast-like MG-63 cells and the stimulatory effects on osteoblast differentiation were noticeably greater for the hot-water extract than for daidzein (a positive control), as reflected by alkaline phosphatase activity, collagen content, and mineral deposition. Expression of the bone-remodeling marker osteocalcin production and bone microstructural parameters were significantly improved in Sprague-Dawley ratsin vivoafter oral treatment with the hot-water extract compared with their control (saline-administered) counterparts. The chemical compounds of the hot-water extract were characterized by liquid chromatography-mass spectrometry, and alliin, sinapic acid, and ferulic acid, which exert beneficial effects on bone health, were identified. These findings indicate thatA. hookerican be used as a natural resource for increasing bone formation. This is the first report of the anabolic effects ofA. hookeriextracts on bone formationin vitroandin vivo.
College of Pharmacy, Chung-Ang University, Dongjak-gu, Seoul, South Korea.
Heajin
H
Park
College of Pharmacy, Chung-Ang University, Dongjak-gu, Seoul, South Korea.
Jaehoon
J
Jeong
College of Pharmacy, Chung-Ang University, Dongjak-gu, Seoul, South Korea.
Hanbit
H
Hyun
College of Pharmacy, Chung-Ang University, Dongjak-gu, Seoul, South Korea.
Jihye
J
Kim
College of Pharmacy, Chung-Ang University, Dongjak-gu, Seoul, South Korea.
Haesung
H
Kim
College of Pharmacy, Chung-Ang University, Dongjak-gu, Seoul, South Korea.
Hyun Il
HI
Oh
College of Pharmacy, Chung-Ang University, Dongjak-gu, Seoul, South Korea.
Jai Yeon
JY
Choi
College of Pharmacy, Chung-Ang University, Dongjak-gu, Seoul, South Korea.
Hye Seong
HS
Hwang
Korea Research Institute of Bioscience & Biotechnology (KRIBB), Yuseong-gu, Daejeon, South Korea.
Doo Byung
DB
Oh
School of Life Sciences, Gwangju Institute of Science and Technology, Oryong-dong, Buk-gu, Gwangju, South Korea.
Jae Il
JI
Kim
College of Pharmacy, Chung-Ang University, Dongjak-gu, Seoul, South Korea.
Ha Hyung
HH
Kim
D000490:Allium; D000818:Animals; D015519:Bone Density / Q000187:drug effects*; D001842:Bone and Bones / Q000000981:diagnostic imaging / Q000187:drug effects*; D002460:Cell Line; D004353:Drug Evaluation, Preclinical; D005260:Female; D006801:Humans; D010012:Osteogenesis / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D017207:Rats, Sprague-Dawley; D055114:X-Ray Microtomography
D010936:Plant Extracts