NMR as a tool to investigate the structure, dynamics and function of membrane proteins.
Membrane-protein NMR occupies a unique niche for determining structures, assessing dynamics, examining folding, and studying the binding of lipids, ligands and drugs to membrane proteins. However, NMR analyses of membrane proteins also face special challenges that are not encountered with soluble proteins, including sample preparation, size limitation, spectral crowding and sparse data accumulation. This Perspective provides a snapshot of current achievements, future opportunities and possible limitations in this rapidly developing field.
Center for Membrane and Cell Physiology, University of Virginia School of Medicine, Charlottesville, Virginia, USA.|Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, Virginia, USA.
Binyong
B
Liang
Center for Membrane and Cell Physiology, University of Virginia School of Medicine, Charlottesville, Virginia, USA.|Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, Virginia, USA.
Lukas K
LK
Tamm
D018829:Adhesins, Bacterial / Q000737:chemistry* / Q000502:physiology; D000818:Animals; D001105:Archaea / Q000737:chemistry; D001419:Bacteria / Q000737:chemistry; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000502:physiology; D025602:Halorhodopsins / Q000737:chemistry* / Q000502:physiology; D008024:Ligands; D008051:Lipid Bilayers / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry; D051379:Mice; D008823:Micelles; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D055553:Prescription Drugs / Q000737:chemistry; D011485:Protein Binding; D011487:Protein Conformation; D000072417:Protein Domains; D017510:Protein Folding; D017433:Protein Structure, Secondary; D018079:Receptors, GABA / Q000737:chemistry* / Q000502:physiology; D025603:Sensory Rhodopsins / Q000737:chemistry* / Q000502:physiology
D018829:Adhesins, Bacterial; D001425:Bacterial Outer Membrane Proteins; C463819:Bzrp protein, mouse; D025602:Halorhodopsins; D008024:Ligands; D008051:Lipid Bilayers; D008055:Lipids; D008823:Micelles; C121501:OprG protein, Pseudomonas aeruginosa; D055553:Prescription Drugs; D018079:Receptors, GABA; D025603:Sensory Rhodopsins; C064832:YadA protein, Yersinia; C061508:sensory rhodopsin II protein, archaeal