Effects of TGF-β1 on the Proliferation and Apoptosis of Human Cervical Cancer Hela Cells In Vitro.
To investigate the effects of TGF-β1 on the proliferation and apoptosis of cervical cancer Hela cells in vitro. Human cervical cancer Hela cells were cultured in vitro and divided into the experimental and control groups. In the experimental groups, Hela cells were stimulated with different concentrations of TGF-β1 (0.01, 0.1, 1, and 10 ng/mL), while Hela cells cultured in serum-free medium without TGF-β1 were used as controls. The CCK8 method was adopted to detect the effect of TGF-β1 on Hela cell proliferation, and flow cytometry was used to determine cell apoptosis 72 h after TGF-β1 treatment. Compared with the control group, the CCK-8 tests showed that different concentrations of TGF-β1 had no obvious effect on Hela cell proliferation 24 h after treatment (P > 0.05). However, upon 48 or 72 h of treatment, TGF-β1 significantly inhibited the proliferation of Hela cells in a time- and dose-dependent manner (P < 0.05). The flow cytometry results indicated that TGF-β1 influenced the apoptosis of human cervical cancer Hela cells in a dose-dependent manner after 72 h of treatment (P < 0.05). TGF-β1 significantly inhibited the growth and induced the apoptosis of human cervical Hela cells in vitro.
Department of Oncology, The Affiliated Hospital of Luzhou Medical College, Luzhou, 646000, People's Republic of China.
Ming-Zhu
MZ
Tao
Department of Pathology, The Affiliated Hospital of Luzhou Medical College, 25 Taiping Street, Jiangyang District, Luzhou, 646000, Sichuan, People's Republic of China.
Xia
X
Gao
Department of Pathology, The Affiliated Hospital of Luzhou Medical College, 25 Taiping Street, Jiangyang District, Luzhou, 646000, Sichuan, People's Republic of China.
Tie-Jun
TJ
Zhou
Department of Pathology, The Affiliated Hospital of Luzhou Medical College, 25 Taiping Street, Jiangyang District, Luzhou, 646000, Sichuan, People's Republic of China.
Qing-Xi
QX
Guo
Department of Pathology, The Affiliated Hospital of Luzhou Medical College, 25 Taiping Street, Jiangyang District, Luzhou, 646000, Sichuan, People's Republic of China.
Qiang
Q
Zhang
Department of Pathology, The Affiliated Hospital of Luzhou Medical College, 25 Taiping Street, Jiangyang District, Luzhou, 646000, Sichuan, People's Republic of China. ycwty2013@163.com.
Cheng-Wan
CW
Yang
D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D006367:HeLa Cells; D006801:Humans; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D016212:Transforming Growth Factor beta
Apoptosis; Cell culture; Cervical carcinoma; Proliferation; TGF-β1