[Effect of MT01/PEN complexes on the expression of osteoprotegerin and receptor activator of nuclear factor κB ligand in human osteoblast-like cell line MG63].
OBJECTIVE
This study aims to synthesize MTO1 (a kind of oligodeoxynucleotides) and N-isopropylacrylamide-modified polyethylenimines (PEN) complexes (MT01/PEN) as well as to investigate the effect of the complexes on the expression of osteoprotegerin (OPG) and the receptor activator of nuclear factor κB ligand (RANKL) in the human osteoblast-like cell line MG63.


METHODS
MG63 cells were transfected by MT01/PEN complexes formed with three different mass ratios (1:2, 1:4, 1:6) of MT01 to PEN. MT01 and MT01-s were used as positive control. Enzyme-linked immunosorbent assay and real-time polymerase chain reaction were performed to estimate the amount of OPG and RANKL released into the culture media and in MG63 at 24, 48, 72 h.


RESULTS
MG63 responded to the MT01/PEN complexes by significantly upregulating the OPG on the protein and mRNA levels (P < 0.05). The protein and mRNA levels of RANKL were lower in most of the groups with complexes, and the OPG/RANKL ratio were higher (P < 0.05). MG63 were affected by the MT01/PEN complexes with different mass ratios, particularly when the ratio was 1:6.


CONCLUSIONS
MT01 can enhance the promotion of ossification by establishing the delivery system with PEN.
Cui
C
Ye
Zheng
Z
Yi
Shen
S
Yuqin
Hou
H
Xu
Lou
L
Yixin
Sun
S
Xinhua
D000178:Acrylamides; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D009838:Oligodeoxyribonucleotides; D010006:Osteoblasts; D053244:Osteoprotegerin; D011094:Polyethyleneimine; D053245:RANK Ligand; D012333:RNA, Messenger; D060888:Real-Time Polymerase Chain Reaction
D000178:Acrylamides; D009838:Oligodeoxyribonucleotides; D053244:Osteoprotegerin; D053245:RANK Ligand; D012333:RNA, Messenger; D011094:Polyethyleneimine; C067295:N-isopropylacrylamide