The Study of Cellular and Molecular Physiological Characteristics of Sperm in Men Living in the Aral Sea Region.
BACKGROUND
Extreme environmental situation in the Aral crisis has caused a massive chemical pollution of the territory for decades with high doses of pesticides, herbicides. Discharge of industrial waste into the rivers that feed the Aral Sea has lead to the development of various pathological processes in the human body, as well as disruption of reproductive function in young men.


OBJECTIVE
To evaluate the performance of molecular cellular changes in the sperm of men under the influence of dust and salt aerosols in Aral Sea region.


METHODS
Clinical and laboratory studies were conducted in men 5 settlements (Aralsk-city, v. Aiteke-Bi, v. Zhalagash, v. Zhusaly, v. Shieli). We have studied male ejaculate obtained after 4-5 days of abstinence, and placed it in a warm tube with a glass stopper. On the investigation proceeded ejaculate within 20-30 minutes after its preparation, during which time he was subjected to liquefaction. Isolation and quantification of ASF, RNA, DNA, and determining the fraction of histones in sperm was performed by the method of Markusheva and Savina.


RESULTS
It was found that the value of ASF in the semen of men living in the zone of ecological disaster higher compared with the values of parameters in men living in the area of environmental crisis, and this trend is observed in all age groups. The study of circulating extracellular DNA and RNA in the sperm of men registered their decline with a corresponding increase of acid precursors that can be attributed to the degradation of nucleic acids under the influence of negative factors in the complex area of ecological trouble. Also, according to a study in men residing in the areas of environmental catastrophe at the age of 18-29 years, found an increased content of the H1 histone H2A lower total fraction, H3, H4 - and a sharp increase in histone H2B content - histones.


CONCLUSIONS
Men living in environmentally disadvantaged areas of Kyzylorda region under the influence of dust and salt aerosols and other toxicants leads to disruption of the reproductive function in men.
Karaganda State Medical University, Department of the Republic of Kazakhstan, Gogolya street 40, Karaganda, Kazakhstan.
Berikbay Z
BZ
Kultanov
Karaganda State Medical University, Department of the Republic of Kazakhstan, Gogolya street 40, Karaganda, Kazakhstan.
Raushan S
RS
Dosmagambetova
Karaganda State Medical University, Department of the Republic of Kazakhstan, Gogolya street 40, Karaganda, Kazakhstan.
Svetlana A
SA
Ivasenko
Karaganda State Medical University, Department of the Republic of Kazakhstan, Gogolya street 40, Karaganda, Kazakhstan.
Yelena S
YS
Tatina
Karaganda State Medical University, Department of the Republic of Kazakhstan, Gogolya street 40, Karaganda, Kazakhstan.
Assel A
AA
Kelmyalene
Karaganda State Medical University, Department of the Republic of Kazakhstan, Gogolya street 40, Karaganda, Kazakhstan.
Lyazzat H
LH
Assenova
ASF; Aral Sea; DNA; H1; H2A; H3; H4; RNA