Identification and tissue expression profile of genes from three chemoreceptor families in an urban pest, Periplaneta americana.
Periplaneta americana is a notorious urban pest prevalent in human habitats; very little is known about its chemosensory mechanism. Employing the advanced next-generation sequencing technique, in the present study, we conducted transcriptome sequencing and analysis of the antennae of the adult males and females as well as their mouthparts using an Illumina platform. This resulted in the discovery of a huge number of the members of all major known chemosensory receptor families in P. americana, including 96 odorant receptors (ORs), 53 ionotropic receptors (IRs), and 33 gustatory receptors (GRs). Tissue expression profiles showed most of them mainly expressed in antennae and phylogenetic analysis demonstrated the expansion in the clade distinguishing them from other functionally well-known Lepidoptera species. A high percentage of chemosensory receptor genes (ORs in particular) showing female antenna bias in mRNA expression was observed. Our results provide a basis for further investigations on how P. americana coordinates its chemosensory receptor genes in chemical communication with environments, and for development of novel pest management approaches.
Key Lab of Optimal Utilization of Natural Medicine Resources, Department of Pharmacology of Chinese Material Medica, Guizhou Medical University, Huaxi District, Guiyang, Guizhou 550025, People's Republic of China.
Yan
Y
Chen
State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Huaxi District, Guiyang 550025, People's Republic of China.
Ming
M
He
Key Laboratory of Tea Biology and Resource Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Science, Hangzhou, 310008, People's Republic of China.
Zhao-Qun
ZQ
Li
College of Life Sciences, Huaibei Normal University, Huaibei, 235000, People's Republic of China.
Ya-Nan
YN
Zhang
State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Huaxi District, Guiyang 550025, People's Republic of China.
Peng
P
He
D000818:Animals; D057506:Arthropod Antennae / Q000378:metabolism; D002628:Chemoreceptor Cells / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D058977:Molecular Sequence Annotation / Q000379:methods; D010526:Periplaneta / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism; D059467:Transcriptome / Q000235:genetics*
D019476:Insect Proteins; D011956:Receptors, Cell Surface