Magnetic Fe3O4 nanoparticle catalyzed chemiluminescence for detection of nitric oxide in living cells.
Direct and real-time measurement of nitric oxide (NO) in biological media is very difficult due to its transient nature. Fe3O4 nanoparticles (nanoFe3O4) because of their unique catalytic activities have attracted much attention as catalysts in a variety of organic and inorganic reactions. In this work, we have developed a magnetic Fe3O4 nanoparticle-based rapid-capture system for real-time detection of cellular NO. The basic principle is that the nanoFe3O4 can catalyze the decomposition of H2O2 in the system to generate superoxide anion (O2 (·-)) and the O2 (·-) can serve as an effective NO(·) trapping agent yielding peroxynitrite oxide anion, ONOO(-). Then the concentration of NO in cells can be facilely determined via peroxynitrite-induced luminol chemiluminescence. The linear range of the method is from 10(-4) to 10(-8) mol/L, and the detection of limit (3σ, n = 11) is as low as 3.16 × 10(-9) mol/L. By using this method, the NO concentration in 0.1 and 0.5 mg/L LPS-stimulated BV2 cells was measured as 4.9 and 11.3 μM, respectively. Surface measurements by synchrotron X-ray photoelectron spectroscopy (SRXPS) and scanning transmission X-ray microscopy (STXM) demonstrate the catalytic mechanism of the nanoFe3O4-based system is that the significantly excess Fe(II) exists on the surface of nanoFe3O4 and mediates the rapid heterogeneous electron transfer, thus presenting a new Fe2O3 phase on the surface.
School of Materials Science and Engineering, Qilu University of Technology, 3501 Daxue Rd., Jinan, Shandong, 250353, China.|Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, 19B Yu Quan Road, Shi JIng Shan District,, Beijing, 100049, China.
Huiliang
H
Wang
School of Materials Science and Engineering, Qilu University of Technology, 3501 Daxue Rd., Jinan, Shandong, 250353, China. lim@spu.edu.cn.
Mei
M
Li
Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, 19B Yu Quan Road, Shi JIng Shan District,, Beijing, 100049, China. wangbing@ihep.ac.cn.
Bing
B
Wang
Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, 19B Yu Quan Road, Shi JIng Shan District,, Beijing, 100049, China.
Meng
M
Wang
Beijing Synchrotron Radiation Facility, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, 100049, China.
Ibrahim
I
Kurash
Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, 4288 Shuangzhu Hwy, Jiading,, Shanghai, 20180, China.
Xiangzhi
X
Zhang
Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, 19B Yu Quan Road, Shi JIng Shan District,, Beijing, 100049, China.
Weiyue
W
Feng
D000818:Animals; D002384:Catalysis; D002460:Cell Line; D008163:Luminescent Measurements / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D051379:Mice; D017628:Microglia / Q000378:metabolism*; D057054:Molecular Imaging / Q000379:methods*; D009569:Nitric Oxide / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D058185:Magnetite Nanoparticles; D009569:Nitric Oxide
Fe3O4 nanoparticle; Intracellular nitric oxide detection; Rapid capture