[Calcitonin gene-related peptide inhibits the expression of Nod-like receptor protein 3 to Dromote osteoblast differentiation in mouse osteoblasts in vitro].
OBJECTIVE
This study aims to investigate the regulatory effects of calcitonin gene-related peptide (CGRP) on Nod-like receptor protein 3 (NLRP3) and interleukin-1β (IL-1β) to promote osteoblast differentiation.


METHODS
Different concentrations of CGRP (0, 10, 30, 100 ng · mL⁻¹) were added to mouse osteoblasts in vitro. The mRNA and protein expression levels of both NLRP3 and IL-1β were examined using Real-time polymerase chain reaction and Western blot, respectively. Moreover, the concentrations of IL-1β protein and intracellular reactive oxygen species (ROS) were detected using enzyme-linked immunosorbent assay and flow cytometry, respectively. The osteogenic differentiation of mouse osteoblasts was identified through alizarin red staining.


RESULTS
The protein and mRNA expression levels of both NLRP3 and IL-1β significantly decreased (P < 0.05) with increasing CGRP concentration. Moreover, the contents of intracellular ROS gradually decreased (P<0.05). The osteogenic differentiation of the osteoblasts was more enhanced in the group treated with 100 ng · mL⁻¹ CGRP than in the empty group (0 ng · mL⁻¹ CGRP).


CONCLUSIONS
CGRP promotes osteoblast differentiation by inhibiting the expression of inflammatory factors.
Cai
C
Jun
Lü
L
Jun
Li
L
Shiting
Gao
G
Qiangguo
Zhang
Z
Gang
D000818:Animals; D015153:Blotting, Western; D002116:Calcitonin; D015740:Calcitonin Gene-Related Peptide; D002454:Cell Differentiation; D004797:Enzyme-Linked Immunosorbent Assay; D053583:Interleukin-1beta; D051379:Mice; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; D000070576:NLR Proteins; D010006:Osteoblasts; D010012:Osteogenesis; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D060888:Real-Time Polymerase Chain Reaction
D053583:Interleukin-1beta; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; D000070576:NLR Proteins; C492716:Nlrp3 protein, mouse; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D002116:Calcitonin; D015740:Calcitonin Gene-Related Peptide