Chlorhexidine-calcium phosphate nanoparticles - Polymer mixer based wound healing cream and their applications.
In this work, we developed a wound healing cream composed of two different polymers, namely chitosan and gelatin with chlorhexidine along with calcium phosphate nanoparticles. The physicochemical properties of the prepared cream were investigated based on SEM, EDX, Raman, FTIR and the results indicated that the cream contained gelatin, chitosan, calcium phosphate nanoparticles and chlorhexidine. The maximum swelling ratio studies indicated that the ratio was around of 52±2.2 at pH7.4 and the value was increased in acidic and alkaline pH. The antimicrobial activity was tested against bacteria and the results indicated that, both chlorhexidine and the hybrid cream devoid of chlorhexidine exhibited antimicrobial activity but the chlorhexidine impregnated cream showed three fold higher antimicrobial activity than without chlorhexidine. In vivo wound healing promoting activities of hybrid cream containing 0.4mg/L chlorhexidine were evaluated on surgically induced dermal wounds in mice. The results indicated that the cream with incorporated chlorhexidine significantly enhanced healing compared with the control samples. For the field validations, the veterinary clinical animals were treated with the cream and showed enhanced healing capacity. In conclusion, a simple and efficient method for design of a novel wound healing cream has been developed for veterinary applications.
Translational Research Platform for Veterinary Biologicals, Centre for Animal Health Studies (CAHS), Tamil Nadu Veterinary and Animal Sciences University (TANUVAS), Chennai 600051, India. Electronic address: viswanathanphd@yahoo.com.
Kaliyaperumal
K
Viswanathan
Translational Research Platform for Veterinary Biologicals, Centre for Animal Health Studies (CAHS), Tamil Nadu Veterinary and Animal Sciences University (TANUVAS), Chennai 600051, India.
P
P
Monisha
Translational Research Platform for Veterinary Biologicals, Centre for Animal Health Studies (CAHS), Tamil Nadu Veterinary and Animal Sciences University (TANUVAS), Chennai 600051, India.
M
M
Srinivasan
Translational Research Platform for Veterinary Biologicals, Centre for Animal Health Studies (CAHS), Tamil Nadu Veterinary and Animal Sciences University (TANUVAS), Chennai 600051, India.
D
D
Swathi
Translational Research Platform for Veterinary Biologicals, Centre for Animal Health Studies (CAHS), Tamil Nadu Veterinary and Animal Sciences University (TANUVAS), Chennai 600051, India.
M
M
Raman
Translational Research Platform for Veterinary Biologicals, Centre for Animal Health Studies (CAHS), Tamil Nadu Veterinary and Animal Sciences University (TANUVAS), Chennai 600051, India. Electronic address: dhinakarrajg@tanuvas.org.in.
G
G
Dhinakar Raj
D000818:Animals; D002130:Calcium Phosphates / Q000737:chemistry / Q000494:pharmacology; D002710:Chlorhexidine / Q000737:chemistry / Q000494:pharmacology; D004195:Disease Models, Animal; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000737:chemistry*; D063465:Skin Cream / Q000737:chemistry / Q000494:pharmacology; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000188:drug therapy* / Q000473:pathology
D002130:Calcium Phosphates; C020243:calcium phosphate; D002710:Chlorhexidine
Calcium phosphate; Chitosan; Gelatin; Wound healing