13C Natural Abundance of Serum Retinol Is a Novel Biomarker for Evaluating Provitamin A Carotenoid-Biofortified Maize Consumption in Male Mongolian Gerbils.
Crops such as maize, sorghum, and millet are being biofortified with provitamin A carotenoids to ensure adequate vitamin A (VA) intakes. VA assessment can be challenging because serum retinol concentrations are homeostatically controlled and more sensitive techniques are resource-intensive.



We investigated changes in serum retinol relative differences of isotope amount ratios of (13)C/(12)C (δ(13)C) caused by natural (13)C fractionation in C3 compared with C4 plants as a biomarker to detect provitamin A efficacy from biofortified (orange) maize and high-carotene carrots.



The design was a 2 × 2 × 2 maize (orange compared with white) by carrot (orange compared with white) by a VA fortificant (VA+ compared with VA-) in weanling male Mongolian gerbils (n = 55), which included a 14-d VA depletion period and a 62-d treatment period (1 baseline and 8 treatment groups; n = 5-7/group). Liver VA and serum retinol were quantified, purified by HPLC, and analyzed by GC combustion isotope ratio mass spectrometry for (13)C.



Treatments affected liver VA concentrations (0.048 ± 0.039 to 0.79 ± 0.24 μmol/g; P < 0.0001) but not overall serum retinol concentrations (1.38 ± 0.22 μmol/L). Serum retinol and liver VA δ(13)C were significantly correlated (R(2) = 0.92; P < 0.0001). Serum retinol δ(13)C differentiated control groups that consumed white maize and white carrots (-27.1 ± 1.2 δ(13)C‰) from treated groups that consumed orange maize and white carrots (-21.6 ± 1.4 δ(13)C‰ P < 0.0001) and white maize and orange carrots (-30.6 ± 0.7 δ(13)C‰ P < 0.0001). A prediction model demonstrated the relative contribution of orange maize to total dietary VA for groups that consumed VA from mixed sources.



Provitamin A efficacy and quantitative estimation of the relative contribution to dietary VA were demonstrated with the use of serum retinol δ(13)C. This method could be used for maize efficacy or effectiveness studies and with other C4 crops biofortified with provitamin A carotenoids (e.g., millet, sorghum). Advantages include no extrinsic tracer dose, 1 blood sample, and higher sensitivity than serum retinol concentrations alone.
Department of Nutritional Sciences, Interdepartmental Graduate Program in Nutritional Sciences.
Bryan M
0000-0003-1781-9242
BM
Gannon
Department of Nutritional Sciences, Interdepartmental Graduate Program in Nutritional Sciences.
India
I
Pungarcher
Department of Nutritional Sciences, Interdepartmental Graduate Program in Nutritional Sciences.
Luciana
L
Mourao
Department of Nutritional Sciences, Interdepartmental Graduate Program in Nutritional Sciences.
Christopher R
CR
Davis
Department of Horticulture, Vegetable Crops Research Unit, and.
Philipp
P
Simon
Department of Agronomy, University of Wisconsin, Madison, WI; and International Maize and Wheat Improvement Center, Texcoco, Mexico.
Kevin V
KV
Pixley
Department of Nutritional Sciences, Interdepartmental Graduate Program in Nutritional Sciences, sherry@nutrisci.wisc.edu.
Sherry A
SA
Tanumihardjo
D000818:Animals; D015415:Biomarkers / Q000097:blood; D002244:Carbon / Q000378:metabolism*; D002247:Carbon Isotopes; D002338:Carotenoids / Q000737:chemistry / Q000378:metabolism*; D018552:Daucus carota; D005527:Food, Fortified; D005849:Gerbillinae; D006801:Humans; D008297:Male; D030821:Plants, Genetically Modified; D000072664:Provitamins / Q000737:chemistry / Q000378:metabolism*; D014801:Vitamin A / Q000097:blood* / Q000378:metabolism; D003313:Zea mays / Q000378:metabolism*
D015415:Biomarkers; D002247:Carbon Isotopes; D000072664:Provitamins; D014801:Vitamin A; D002338:Carotenoids; D002244:Carbon
GCCIRMS; biofortification; carrot; stable carbon isotope; vitamin A effectiveness; vitamin A efficacy; vitamin A+