Impact of high-fat diet on liver genes expression profiles in mice model of nonalcoholic fatty liver disease.
Evidences have shown that NAFLD influences expression of some drug metabolic enzyme genes. This study aims to investigate the role of HFD-induced NAFLD in regulating the transcription of genes, particularly the drug metabolizing genes variation. Transcriptome analysis demonstrated that HFD feeding caused the 150 genes expression to change, most genes associated with lipid metabolism, inflammatory, oxidative stress and oxidoreductase activity up-regulated, whereas most genes involved in nucleic acid metabolism repressed. The genes involved in drug metabolism had 16 down-regulated and 21 up-regulated in NAFLD. The over-4-fold change genes included the down-regulation of Cyp8b1, Cyp7a1, Sult3a1, Sult1e1, Cyp17a1, Cyp3a41a, Gstt3, Cyp51, Cyp2c54 and Cyp4f14, and the up-regulation of Asns, Past1, Cyp2c55, Gstm2, Cyp2e1 and Gstaα1. In conclusion, significant alterations in the expression of drug metabolizing enzymes may affect the clearance of therapeutic drugs, with the potential to result in adverse drug reactions or drug toxicity in nonalcoholic fatty liver disease.
Department of Basic Veterinary Science, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China; Faculty of Life Science and Technology, Mudanjiang Normal University, Mudanjiang 157012, PR China.
Chunhua
C
Wang
Department of Basic Veterinary Science, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China; State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Harbin 150001, PR China.
Qimeng
Q
Tao
Department of Basic Veterinary Science, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China.
Xinghe
X
Wang
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Harbin 150001, PR China.
Xiurong
X
Wang
Department of Basic Veterinary Science, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China. Electronic address: zhangxiuying@neau.edu.cn.
Xiuying
X
Zhang
D000818:Animals; D059305:Diet, High-Fat; D004195:Disease Models, Animal; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008810:Mice, Inbred C57BL; D065626:Non-alcoholic Fatty Liver Disease / Q000235:genetics* / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D059467:Transcriptome
D012333:RNA, Messenger
Cytochrome P450; Drug metabolizing enzymes; Liver gene expression; Nonalcoholic fatty liver disease