Proteomic characterization of hempseed (Cannabis sativa L.).
This paper presents an investigation on hempseed proteome. The experimental approach, based on combinatorial peptide ligand libraries (CPLLs), SDS-PAGE separation, nLC-ESI-MS/MS identification, and database search, permitted identifying in total 181 expressed proteins. This very large number of identifications was achieved by searching in two databases: Cannabis sativa L. (56 gene products identified) and Arabidopsis thaliana (125 gene products identified). By performing a protein-protein association network analysis using the STRING software, it was possible to build the first interactomic map of all detected proteins, characterized by 137 nodes and 410 interactions. Finally, a Gene Ontology analysis of the identified species permitted to classify their molecular functions: the great majority is involved in the seed metabolic processes (41%), responses to stimulus (8%), and biological process (7%).



Hempseed is an underexploited non-legume protein-rich seed. Although its protein is well known for its digestibility, essential amino acid composition, and useful techno-functional properties, a comprehensive proteome characterization is still lacking. The objective of this work was to fill this knowledge gap and provide information useful for a better exploitation of this seed in different food products.
Department of Pharmaceutical Sciences, University of Milan, via Mangiagalli 25, 20133 Milan, Italy.
Gilda
G
Aiello
Department of Chemistry, Materials and Chemical Engineering "Giulio Natta", Polytechnic of Milan, via Mancinelli 7, 20131 Milan, Italy.
Elisa
E
Fasoli
Department of Pharmaceutical Sciences, University of Milan, via Mangiagalli 25, 20133 Milan, Italy.
Giovanna
G
Boschin
Department of Pharmaceutical Sciences, University of Milan, via Mangiagalli 25, 20133 Milan, Italy.
Carmen
C
Lammi
Department of Pharmaceutical Sciences, University of Milan, via Mangiagalli 25, 20133 Milan, Italy.
Chiara
C
Zanoni
Department of Chemistry, Materials and Chemical Engineering "Giulio Natta", Polytechnic of Milan, via Mancinelli 7, 20131 Milan, Italy.
Attilio
A
Citterio
Department of Pharmaceutical Sciences, University of Milan, via Mangiagalli 25, 20133 Milan, Italy. Electronic address: anna.arnoldi@unimi.it.
Anna
A
Arnoldi
D002188:Cannabis / Q000737:chemistry*; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D019151:Peptide Library; D010940:Plant Proteins / Q000032:analysis; D060066:Protein Interaction Maps; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry
D019151:Peptide Library; D010940:Plant Proteins; D020543:Proteome
Cannabis sativa; Foodomics; Hempseed; Interactomic maps