MicroRNA-505 suppresses proliferation and invasion in hepatoma cells by directly targeting high-mobility group box 1.
OBJECTIVE
MicroRNA-505 (miR-505) expressions have been reported to be altered in the serum of HCC patients. However, the effect and underlying mechanism of miR-505 in hepatoma cells remains poorly understood. The present study intended to investigate the expression levels and the probable role and molecular basis of miR-505 in hepatoma cells.


METHODS
Real-time PCR was used to determine the miR-505 expressions in hepatoma cell lines QGY-7703, SMMC-7721 and MHCC97. Furthermore, an up-or down-regulation of miR-505 was performed in MHCC97 by transfected with miR-505 mimics or anti-miR-505, respectively. Cell proliferation, cell invasion, and epithelial-mesenchymal transition were determined. Moreover, the target gene of miR-505 was also investigated.


RESULTS
The expressions of miR-505 were down-regulated in three hepatoma cell lines. MHCC97 possessed the lowest miR-505 levels among the three hepatoma cell lines. Furthermore, the up-regulation of miR-505 suppressed, whereas the down-regulation of miR-505 promoted proliferation, invasion and epithelial-mesenchymal transition in MHCC97. Moreover, miR-505 could directly bind to the 3'-untranslated region of High-Mobility Group Box 1. Notably, High-Mobility Group Box 1 knockdown apparently promoted cell proliferation and invasion in MHCC97.


CONCLUSIONS
We investigated that MiR-505 regulates proliferation and invasion in MHCC97 cells via targeting High-Mobility Group Box 1.
Department of General Surgery, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121001, PR China.
Lin
L
Lu
Department of General Surgery, Jinzhou Central Hospital, Jinzhou, Liaoning 121001, PR China.
Chunyu
C
Qiu
Department of General Surgery, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121001, PR China. Electronic address: ldsh_medical@sina.com.
Dongsheng
D
Li
Department of General Surgery, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning 121001, PR China.
Guang
G
Bai
Department of Hepatobiliary Surgery, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning 110032, PR China.
Jian
J
Liang
Liaoning Medical University Graduate School, Jinzhou, Liaoning 121001, PR China.
Qing
Q
Yang
D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology*; D015536:Down-Regulation; D024243:HMGB1 Protein / Q000378:metabolism*; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D035683:MicroRNAs / Q000502:physiology*; D009361:Neoplasm Invasiveness
D024243:HMGB1 Protein; C585491:HMGB1 protein, human; C574053:MIRN505 microRNA, human; D035683:MicroRNAs
Hepatoma cells; High-Mobility Group Box 1; Invasion; MicroRNA-505; Proliferation