Differentiation of Human Mesenchymal Stem Cells Toward Quality Cartilage Using Fibrinogen-Based Nanofibers.
Mimicking the complex intricacies of the extra cellular matrix including 3D configurations and aligned fibrous structures were traditionally perused for producing cartilage tissue from stem cells. This study shows that human adipose derived mesenchymal stem cells (hADMSCs) establishes significant chondrogenic differentiation and may generate quality cartilage when cultured on 2D and randomly oriented fibrinogen/poly-lactic acid nanofibers compared to 3D sandwich-like environments. The adhering cells show well-developed focal adhesion complexes and actin cytoskeleton arrangements confirming the proper cellular interaction with either random or aligned nanofibers. However, quantitative reverse transcription-polymerase chain reaction analysis for Collagen 2 and Collagen 10 genes expression confirms favorable chondrogenic response of hADMSCs on random nanofibers and shows substantially higher efficacy of their differentiation in 2D configuration versus 3D constructs. These findings introduce a new direction for cartilage tissue engineering through providing a simple platform for the routine generation of transplantable stem cells derived articular cartilage replacement that might improve joint function.
BioElpida, Lyon, France.
Jeremy
J
Forget
Molecular Dynamics at Cell Biomaterial Interface, Institute for Bioengineering of Catalonia (IBEC), Barcelona, Spain.
Firas
F
Awaja
Molecular Dynamics at Cell Biomaterial Interface, Institute for Bioengineering of Catalonia (IBEC), Barcelona, Spain.
Dencho
D
Gugutkov
Molecular Dynamics at Cell Biomaterial Interface, Institute for Bioengineering of Catalonia (IBEC), Barcelona, Spain.
Juhan
J
Gustavsson
Center for Biomaterials and Tissue Engineering (CBIT), Universitat Politècnica de València, Valencia, Spain.|Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain.
Gloria
G
Gallego Ferrer
Molecular Dynamics at Cell Biomaterial Interface, Institute for Bioengineering of Catalonia (IBEC), Barcelona, Spain.|Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.|Institute of Biophysics Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Tatiana
T
Coelho-Sampaio
Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.|Institute of Biophysics Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Camila
C
Hochman-Mendez
Division of Biomedical Engineering, School of Engineering, University of Glasgow, Glasgow, G12 8LT, UK.
Manuel
M
Salmeron-Sánchez
Molecular Dynamics at Cell Biomaterial Interface, Institute for Bioengineering of Catalonia (IBEC), Barcelona, Spain. george.altankov@icrea.cat.|Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain. george.altankov@icrea.cat.|Institucio Catalana de Recerca i Estudis Avançats (ICREA), Barcelona, Spain. george.altankov@icrea.cat.
George
G
Altankov
D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology*; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects*; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D020219:Chondrogenesis / Q000187:drug effects; D003094:Collagen / Q000235:genetics / Q000378:metabolism; D005340:Fibrinogen / Q000494:pharmacology*; D006801:Humans; D021621:Imaging, Three-Dimensional; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D011091:Polyesters / Q000737:chemistry; D060888:Real-Time Polymerase Chain Reaction
D011091:Polyesters; C033616:poly(lactide); D005340:Fibrinogen; D003094:Collagen
FBG/PLA nanofibers; cartilage; chondrogenic response; collagen; mesenchymal stem cells