Silencing of carboxypeptidase E inhibits cell proliferation, tumorigenicity, and metastasis of osteosarcoma cells.
Carboxypeptidase E (CPE), a prohormone processing enzyme, has been implicated in the progression of multiple malignancies. However, the biological role and molecular mechanisms of CPE in osteosarcoma remain elusive. In this study, we assessed the effects of CPE on cell proliferation, tumorigenicity, migration, and invasion in osteosarcoma. Our results showed that silencing of CPE significantly inhibited cell proliferation, caused cell cycle arrest at G0/G1 phase, decreased the expression levels of cell cycle protein, cyclin D1, and inhibited tumorigenicity in vivo. Additionally, CPE downregulation repressed the migratory and invasive capacities of osteosarcoma cells in vitro. Furthermore, overexpression of CPE-ΔN (a splice variant of CPE) enhanced the cell growth, migration, and invasion of osteosarcoma cells. It is possible that both CPE forms are involved in the tumorigenesis and development of osteosarcoma, and therefore CPE may provide a promising biological target for osteosarcoma therapy.
Department of Geriatrics, The First Affiliated Hospital of China Medical University, Shenyang, People's Republic of China.
Shuli
S
Fan
Department of Sports Medicine and Joint Surgery, The First Affiliated Hospital of China Medical University, Shenyang, People's Republic of China.
Xu
X
Li
Department of Sports Medicine and Joint Surgery, The First Affiliated Hospital of China Medical University, Shenyang, People's Republic of China.
Leiming
L
Li
Department of Sports Medicine and Joint Surgery, The First Affiliated Hospital of China Medical University, Shenyang, People's Republic of China.
Liguo
L
Wang
Department of Sports Medicine and Joint Surgery, The First Affiliated Hospital of China Medical University, Shenyang, People's Republic of China.
Zhangzhen
Z
Du
Department of Sports Medicine and Joint Surgery, The First Affiliated Hospital of China Medical University, Shenyang, People's Republic of China.
Yan
Y
Yang
Department of Orthopaedic Surgery, Shengjing Hospital of China Medical University, Shenyang, People's Republic of China.
Jiansong
J
Zhao
Department of Orthopaedic Surgery, Shengjing Hospital of China Medical University, Shenyang, People's Republic of China.
Yan
Y
Li
carboxypeptidase E; invasion; migration; osteosarcoma; proliferation; tumorigenicity