Transcriptome activity of isochores during preimplantation process in human and mouse.
This work investigates the role of isochores during preimplantation process. Using RNA-seq data from human and mouse preimplantation stages, we created the spatio-temporal transcriptional profiles of the isochores during preimplantation. We found that from early to late stages, GC-rich isochores increase their expression while GC-poor ones decrease it. Network analysis revealed that modules with few coexpressed isochores are GC-poorer than medium-large ones, characterized by an opposite expression as preimplantation advances, decreasing and increasing respectively. Our results reveal a functional contribution of the isochores, supporting the presence of structural-functional interactions during maturation and early-embryonic development.
The Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, UK.
Carl
C
Barton
Department of Informatics, King's College London, UK.
Costas S
CS
Iliopoulos
Department of Informatics, King's College London, UK.
Solon P
SP
Pissis
Institute of Molecular Biology and Biotechnology (IMBB), Foundation for Research and Technology - Hellas (FORTH), Heraklion, Crete, Greece.
Stilianos
S
Arhondakis
D000818:Animals; D001755:Blastocyst / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D006801:Humans; D032085:Isochores / Q000378:metabolism*; D051379:Mice; D013045:Species Specificity; D059467:Transcriptome / Q000502:physiology*
D032085:Isochores
GC level; RNA-seq; base composition; expression; isochores; preimplantation; transcriptome