Palladium-Assisted Removal of a Solubilizing Tag from a Cys Side Chain To Facilitate Peptide and Protein Synthesis.
Reversible attachment of solubilizing tags to hydrophobic peptides to facilitate their purification and ligation is an essential yet challenging task in chemical protein synthesis. The efficient palladium-assisted removal of the solubilizing tag linked to the Cys side chain is reported. The strategy was applied for the efficient preparation of histone protein H4 from two fragments via one-pot operation of ligation, removal of the solubilizing tag, and desulfurization.
Schulich Faculty of Chemistry Technion, Israel Institute of Technology , 32000 Haifa, Israel.
Suman Kumar
SK
Maity
Schulich Faculty of Chemistry Technion, Israel Institute of Technology , 32000 Haifa, Israel.
Guy
G
Mann
Schulich Faculty of Chemistry Technion, Israel Institute of Technology , 32000 Haifa, Israel.
Muhammad
M
Jbara
Schulich Faculty of Chemistry Technion, Israel Institute of Technology , 32000 Haifa, Israel.
Shay
S
Laps
Schulich Faculty of Chemistry Technion, Israel Institute of Technology , 32000 Haifa, Israel.
Guy
G
Kamnesky
Schulich Faculty of Chemistry Technion, Israel Institute of Technology , 32000 Haifa, Israel.
Ashraf
A
Brik
D003545:Cysteine / Q000737:chemistry*; D006657:Histones / Q000138:chemical synthesis; D057927:Hydrophobic and Hydrophilic Interactions; D010165:Palladium / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis; D011487:Protein Conformation; D011506:Proteins / Q000138:chemical synthesis*; D012995:Solubility; D012997:Solvents
D006657:Histones; D010455:Peptides; D011506:Proteins; D012997:Solvents; D010165:Palladium; D003545:Cysteine