Hybridization-based aptamer labeling using complementary oligonucleotide platform for PET and optical imaging.
Aptamers are promising next-generation ligands used in molecular imaging and theragnosis. Aptamers are synthetic nucleic acids that can be held together with complementary sequences by base-pair hybridization. In this study, the complementary oligonucleotide (cODN) hybridization-based aptamer conjugation platform was developed to use aptamers as the molecular imaging agent. The cODN was pre-labeled with fluorescent dye or radioisotope and hybridized with a matched sequence containing aptamers in aqueous conditions. The cODN platform-hybridized aptamers exhibited good serum stability and specific binding affinity towards target cancer cells both in vitro and in vivo. These results suggest that the newly designed aptamer conjugation platform offers great potential for the versatile application of aptamers as molecular imaging agents.
Department of Nuclear Medicine, Severance Hospital, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea; Department of Anatomy, Yonsei University Wonju College of Medicine, 20 Ilsan-ro, Wonju 26426, Republic of Korea.
Jun Young
JY
Park
Molecular Imaging Research Center, Research Institute of Radiological and Medical Sciences, Korea Institute of Radiological and Medical Sciences, 75 Nowon-ro, Nowon-gu, Seoul 01812, Republic of Korea.
Tae Sup
TS
Lee
Molecular Imaging Research Center, Research Institute of Radiological and Medical Sciences, Korea Institute of Radiological and Medical Sciences, 75 Nowon-ro, Nowon-gu, Seoul 01812, Republic of Korea.
In Ho
IH
Song
Department of Nuclear Medicine, Severance Hospital, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea.
Ye Lim
YL
Cho
Department of Nuclear Medicine, Severance Hospital, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea.
Ju Ri
JR
Chae
Department of Nuclear Medicine, Severance Hospital, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea.
Mijin
M
Yun
R&D Strategic Planning, Bundang CHA Medical Center, 59 Yatap-ro, Bundang-gu, Seongnam-si, Gyeonggi-do 13496, Republic of Korea.
Hyungu
H
Kang
APGEN Inc, 220, Yeongsin-ro, Yeongdeungpo-gu, Seoul 07228, Republic of Korea.
Jung Hwan
JH
Lee
APGEN Inc, 220, Yeongsin-ro, Yeongdeungpo-gu, Seoul 07228, Republic of Korea.
Jong Hoon
JH
Lim
Department of Anatomy, Yonsei University Wonju College of Medicine, 20 Ilsan-ro, Wonju 26426, Republic of Korea. Electronic address: wch01@yonsei.ac.kr.
Won Gil
WG
Cho
Department of Nuclear Medicine, Severance Hospital, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea. Electronic address: mdkwj@yuhs.ac.
Won Jun
WJ
Kang
D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000493:pharmacokinetics; D001483:Base Sequence; D016466:CHO Cells; D045744:Cell Line, Tumor; D003412:Cricetulus; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000493:pharmacokinetics; D005462:Fluorine Radioisotopes / Q000737:chemistry* / Q000493:pharmacokinetics; D008819:Mice, Nude; D009369:Neoplasms / Q000000981:diagnostic imaging*; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry; D061848:Optical Imaging / Q000379:methods*; D049268:Positron-Emission Tomography / Q000379:methods*; D051381:Rats
D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D005462:Fluorine Radioisotopes; D009841:Oligonucleotides; C000615276:Fluorine-18
Aptamer; Hybridization; Molecular imaging; Oligonucleotide; PET; Radioisotope