Tetrahymena as a Unicellular Model Eukaryote: Genetic and Genomic Tools.
Tetrahymena thermophila is a ciliate model organism whose study has led to important discoveries and insights into both conserved and divergent biological processes. In this review, we describe the tools for the use of Tetrahymena as a model eukaryote, including an overview of its life cycle, orientation to its evolutionary roots, and methodological approaches to forward and reverse genetics. Recent genomic tools have expanded Tetrahymena's utility as a genetic model system. With the unique advantages that Tetrahymena provide, we argue that it will continue to be a model organism of choice.
Department of Cell and Developmental Biology, University of Colorado Anschutz Medical Campus, Aurora, Colorado, 80045 Marisa.Ruehle@ucdenver.edu.
Marisa D
MD
Ruehle
Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, California 93106.
Eduardo
E
Orias
Department of Cell and Developmental Biology, University of Colorado Anschutz Medical Campus, Aurora, Colorado, 80045.
Chad G
CG
Pearson
D017125:Genes, Protozoan; D005821:Genetic Techniques; D013768:Tetrahymena / Q000235:genetics* / Q000254:growth & development / Q000502:physiology
Tetrahymena thermophila; amitosis; ciliates; genetics; model organism; somatic polyploidy