Validation of an automated screening method for persistent organic contaminants in fats and oils by GC×GC-ToFMS.
An screening method, comprised of straightforward sample treatment based on silica clean-up, GC×GC-ToFMS detection and automated data processing with the non-proprietary free downloadable software MetAlignID, has been successfully validated with respect to false negatives for the sum PCB 28, 52, 101, 138, 153 and 180), for the sum of BDE 28, 47, 99, 100, 153, 154 and 183, for the four markers of PAHs and for a number of emerging brominated flame retardants. A screening detection limit (SDL) equal to or lower than the maximum regulatory level was always achieved. MetAlignID considerably decreased the time needed for data treatment from 20 to 5min/file. Automated identification of the signature mass spectral patterns was applied to identify chlorinated- and brominated-containing substances with more than two halogen atoms, and PAH derivates. Although the success rate was variable and needs to be further improved, the tool was considered to be of added value.
RIKILT - Wageningen UR, Akkermaalsbos 2, 6708 WB Wageningen, The Netherlands. Electronic address: patricia.lopezsanchez@wur.nl.
Patricia
P
López
RIKILT - Wageningen UR, Akkermaalsbos 2, 6708 WB Wageningen, The Netherlands.
Marc
M
Tienstra
RIKILT - Wageningen UR, Akkermaalsbos 2, 6708 WB Wageningen, The Netherlands.
Arjen
A
Lommen
RIKILT - Wageningen UR, Akkermaalsbos 2, 6708 WB Wageningen, The Netherlands.
Hans G J
HG
Mol
D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D005411:Flame Retardants / Q000032:analysis; D005506:Food Contamination / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D057230:Limit of Detection; D010938:Plant Oils / Q000032:analysis*; D011075:Polybrominated Biphenyls / Q000032:analysis; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis; D015203:Reproducibility of Results
D005411:Flame Retardants; D010938:Plant Oils; D011075:Polybrominated Biphenyls; D011084:Polycyclic Aromatic Hydrocarbons; C533760:tribromodiphenyl ether 28
Fat; GC×GC-ToFMS; Qualitative analysis; Screening; Validation