Composite particles formed by complexation of poly(methacrylic acid) - stabilized magnetic fluid with chitosan: Magnetic material for bioapplications.
A simple procedure for the synthesis of magnetic fluid (ferrofluid) stabilized by poly(methacrylic acid) has been developed. This ferrofluid was used to prepare a novel type of magnetically responsive chitosan-based composite material. Both ferrofluid and magnetic chitosan composite were characterized by a combination of microscopy (optical microscopy, TEM, SEM), scattering (static and dynamic light scattering, SANS) and spectroscopy (FTIR) techniques. Magnetic chitosan was found to be a perspective material for various bioapplications, especially as a magnetic carrier for immobilization of enzymes and cells. Lipase from Candida rugosa was covalently attached after cross-linking and activation of chitosan using glutaraldehyde. Baker's yeast cells (Saccharomyces cerevisiae) were incorporated into the chitosan composite during its preparation; both biocatalysts were active after reaction with appropriate substrates.
Department of Nanobiotechnology, Biology Centre, ISB, Czech Academy of Sciences, Na Sadkach 7, 37005 Ceske Budejovice, Czech Republic; Regional Centre of Advanced Technologies and Materials, Palacky University, Slechtitelu 27, 78371 Olomouc, Czech Republic; Global Change Research Institute, Czech Academy of Sciences, Na Sadkach 7, 37005 Ceske Budejovice, Czech Republic.
Ivo
I
Safarik
Department of Physical and Macromolecular Chemistry, Faculty of Science, Charles University, Hlavova 2030, 12840 Prague 2, Czech Republic.
Miroslav
M
Stepanek
Department of Physical and Macromolecular Chemistry, Faculty of Science, Charles University, Hlavova 2030, 12840 Prague 2, Czech Republic.
Mariusz
M
Uchman
Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovsky Sq. 2, 16206 Prague 6, Czech Republic.
Miroslav
M
Slouf
Global Change Research Institute, Czech Academy of Sciences, Na Sadkach 7, 37005 Ceske Budejovice, Czech Republic; Department of Applied Chemistry, Faculty of Agriculture, University of South Bohemia, Branisovska 1457, 37005 Ceske Budejovice, Czech Republic.
Eva
E
Baldikova
Global Change Research Institute, Czech Academy of Sciences, Na Sadkach 7, 37005 Ceske Budejovice, Czech Republic.
Leona
L
Nydlova
Regional Centre of Advanced Technologies and Materials, Palacky University, Slechtitelu 27, 78371 Olomouc, Czech Republic.
Kristyna
K
Pospiskova
Department of Nanobiotechnology, Biology Centre, ISB, Czech Academy of Sciences, Na Sadkach 7, 37005 Ceske Budejovice, Czech Republic; Global Change Research Institute, Czech Academy of Sciences, Na Sadkach 7, 37005 Ceske Budejovice, Czech Republic.
Mirka
M
Safarikova
D002175:Candida / Q000201:enzymology*; D018914:Cells, Immobilized / Q000166:cytology / Q000378:metabolism; D048271:Chitosan / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*; D008280:Magnetics; D011109:Polymethacrylic Acids / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000378:metabolism
D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D011109:Polymethacrylic Acids; C030613:polymethacrylic acid; D048271:Chitosan; D008049:Lipase
Chitosan; Immobilization; Lipase; Magnetic fluid; Poly(methacrylic acid)