Arachidonic acid pathway alterations in cerebrospinal fluid of dogs with naturally occurring spinal cord injury.
Canine intervertebral disc πherniation causes a naturally-occurring spinal cord injury (SCI) that bears critical similarities to human SCI with respect to both injury pathomechanisms and treatment. As such, it has tremendous potential to enhance our understanding of injury biology and the preclinical evaluation of novel therapies. Currently, there is limited understanding of the role of arachidonic acid metabolites in canine SCI.



The CSF concentrations of PLA2 and PGE2 were higher in SCI dogs compared to control dogs (p = 0.0370 and 0.0273, respectively), but CSF LCT4 concentration in SCI dogs was significantly lower than that in control dogs (p < 0.0001). Prostaglandin E2 concentration in the CSF was significantly and positively associated with increased severity of SCI at the time of sampling (p = 0.041) and recovery 42 days post-injury (p = 0.006), as measured by ordinal behavioral scores.



Arachidonic acid metabolism is altered in dogs with SCI, and these data suggest that these AA metabolites reflect injury severity and recovery, paralleling data from other model systems.
Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, 77843, USA.|Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, 77843, USA.
Rae L
RL
Russell
Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, 77843, USA.
Jonathan M
JM
Levine
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, 1720 Veterinary Medicine, Ames, IA, 50011, USA.
Nick D
ND
Jeffery
Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, 77843, USA.
Colin
C
Young
Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, 77843, USA.
Armando
A
Mondragon
Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, 77843, USA.
Bryan
B
Lee
Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, 77843, USA.
C Elizabeth
CE
Boudreau
Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, 77843, USA.
C Jane
CJ
Welsh
Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, TAMU 4467, College Station, TX, 77843, USA. GJLevine@cvm.tamu.edu.
Gwendolyn J
GJ
Levine
D000818:Animals; D016718:Arachidonic Acid / Q000134:cerebrospinal fluid* / Q000378:metabolism*; D015415:Biomarkers / Q000134:cerebrospinal fluid; D015232:Dinoprostone / Q000134:cerebrospinal fluid; D004283:Dog Diseases / Q000134:cerebrospinal fluid* / Q000188:drug therapy; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007405:Intervertebral Disc Displacement / Q000134:cerebrospinal fluid / Q000150:complications / Q000188:drug therapy / Q000662:veterinary*; D017997:Leukotriene C4 / Q000134:cerebrospinal fluid; D016014:Linear Models; D008159:Lumbar Vertebrae; D008297:Male; D054467:Phospholipases A2 / Q000134:cerebrospinal fluid; D012720:Severity of Illness Index; D013119:Spinal Cord Injuries / Q000134:cerebrospinal fluid / Q000188:drug therapy / Q000209:etiology / Q000662:veterinary*; D013904:Thoracic Vertebrae
D015415:Biomarkers; D016718:Arachidonic Acid; D017997:Leukotriene C4; D054467:Phospholipases A2; D015232:Dinoprostone
Canine; Neurotrauma; Phospholipase A2; Prostaglandin E2