Dual modification of biomolecules.
With the advent of novel bioorthogonal reactions and "click" chemistry, an increasing number of strategies for the single labelling of proteins and oligonucleotides have emerged. Whilst several methods exist for the site-selective introduction of a single chemical moiety, site-selective and bioorthogonal dual modification of biomolecules remains a challenge. The introduction of multiple modules enables a plethora of permutations and combinations and can generate a variety of bioconjuguates with many potential applications. From de novo approaches on oligomers to the post-translational functionalisation of proteins, this review will highlight the main strategies to dually modify biomolecules.
Department of Chemistry, University College London, 20 Gordon Street, London, WC1H OAJ, UK. antoine.maruani.12@ucl.ac.uk v.chudasama@ucl.ac.uk.
Antoine
A
Maruani
Daniel A
DA
Richards
Vijay
V
Chudasama
D057930:Click Chemistry; D008958:Models, Molecular; D009841:Oligonucleotides / Q000737:chemistry* / Q000378:metabolism; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism
D009841:Oligonucleotides; D011506:Proteins