Protective effect of α-mangostin against iodixanol-induced apoptotic damage in LLC-PK1 cells.
Radiographic contrast media facilitate the visibility of internal body structures, but its use to patients with lowered renal function needs to be careful because of severe side effect in kidney. The present study aims to evaluate potential protective effect and mechanism of Alpha mangostin (α-mangostin) against contrast-induced apoptotic damage in LLC-PK1 cells. As a result, α-mangostin in non-toxic concentrations improved the viability of the iodixanol-treated cells up to 90.42% against contrast-induced damage in LLC-PK1 cells. Iodixanol treatment increased the phosphorylation of p38, ERK and cleavage of caspase-3 in LLC-PK1 cells, which were significantly decreased by co-treatment with α-mangostin (2.5 and 5μM). The protective effect of α-mangostin on contrast-induced apoptotic damage was mediated by the inhibition of MAPKs and caspase activation.
School of Pharmacy, Sungkyunkwan University, Suwon 440-746, Republic of Korea; College of Korean Medicine, Gachon University, Seongnam 13120, Republic of Korea.
Dahae
D
Lee
College of Pharmacy, Dongguk University-Seoul, Gyeonggi 10326, Republic of Korea.
Young Ok
YO
Choi
School of Pharmacy, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
Ki Hyun
KH
Kim
College of Pharmacy, Dongguk University-Seoul, Gyeonggi 10326, Republic of Korea.
Young-Won
YW
Chin
Institute of Pharmaceutical Sciences, College of Pharmacy, CHA University, Sungnam 13844, Republic of Korea.
Hojin
H
Namgung
College of Korean Medicine, Gachon University, Seongnam 13120, Republic of Korea. Electronic address: norikoy@gachon.ac.kr.
Noriko
N
Yamabe
Institute of Pharmaceutical Sciences, College of Pharmacy, CHA University, Sungnam 13844, Republic of Korea. Electronic address: pharmj@cha.ac.kr.
Kiwon
K
Jung
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D018374:LLC-PK1 Cells; D015394:Molecular Structure; D013329:Structure-Activity Relationship; D013552:Swine; D014283:Triiodobenzoic Acids / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D044004:Xanthones / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*
D014283:Triiodobenzoic Acids; D044004:Xanthones; C044834:iodixanol; C021053:mangostin
Alpha mangostin; Caspase; Contrast; Iodixanol; MAPK