Increased RUNX1 expression in patients with immune thrombocytopenia.
Immune thrombocytopenia (ITP) is a heterogeneous autoimmune disease, characterized by dysregulation of cellular immunity. Th17 and associated IL-17 were involved in the pathogenesis of ITP. Runt-related transcription factor 1 (RUNX1), a member of the runt domain-containing family of transcription factors, is required for Th17 differentiation. Whether RUNX1 was involved in the pathogenesis of ITP remains poorly understood. In this study, 30 active ITP patients, 20 ITP in remission and 20 age and gender matched healthy controls were included. Peripheral blood mononuclear cells (PBMCs) were isolated to measure mRNA level of RUNX1 and retinoic acid receptor-related orphan receptor-γt (RORγt) by quantitative real-time PCR and Th17 cells by flow cytometry. Meanwhile, plasma was extracted for measurement of IL-17 level by ELISA. Our results showed a significantly higher expression of RUNX1, RORγt, Th17 cells and plasma level of IL-17 in active ITP patients than that in healthy controls. No differences of expression of RUNX1, RORγt and Th17 cells were observed between remission patients and controls. Furthermore, a significantly positive correlation of RUNX1 with RORγt was found in active ITP patients. In conclusion, RUNX1 was associated with the pathogenesis of ITP possibly through regulation of Th17 cell differentiation and therapeutically targeting it might be a novel approach in ITP treatment.
Department of Medical Oncology, Huai'an First People's Hospital, Nanjing Medical University, Huai'an 223300, China.
Xiaomin
X
Zhong
Blood Diseases Institute, Xuzhou Medical College, Xuzhou 221002, China.
Yulu
Y
Wu
Blood Diseases Institute, Xuzhou Medical College, Xuzhou 221002, China.
Yun
Y
Liu
Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China.
Feng
F
Zhu
Blood Diseases Institute, Xuzhou Medical College, Xuzhou 221002, China.
Xiaoqian
X
Li
Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China.
Depeng
D
Li
Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China.
Zhenyu
Z
Li
Blood Diseases Institute, Xuzhou Medical College, Xuzhou 221002, China; Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China.
Lingyu
L
Zeng
Blood Diseases Institute, Xuzhou Medical College, Xuzhou 221002, China; Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China.
Jianlin
J
Qiao
Department of Medical Oncology, Huai'an First People's Hospital, Nanjing Medical University, Huai'an 223300, China. Electronic address: cxf607001@163.com.
Xiaofei
X
Chen
The First Clinical Medical College, Nanjing Medical University, Nanjing 210029, China; Blood Diseases Institute, Xuzhou Medical College, Xuzhou 221002, China; Department of Hematology, The Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China. Electronic address: lihmd@163.com.
Kailin
K
Xu
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002454:Cell Differentiation; D002478:Cells, Cultured; D050676:Core Binding Factor Alpha 2 Subunit / Q000235:genetics / Q000378:metabolism*; D018450:Disease Progression; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D020381:Interleukin-17 / Q000097:blood; D008297:Male; D008875:Middle Aged; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3 / Q000235:genetics / Q000378:metabolism; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000235:genetics / Q000378:metabolism*; D058504:Th17 Cells / Q000276:immunology*; D055815:Young Adult
D050676:Core Binding Factor Alpha 2 Subunit; D020381:Interleukin-17; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3; C539900:RORC protein, human; C493728:RUNX1 protein, human
IL-17; Immune thrombocytopenia; Retinoic acid receptor-related orphan receptor-γt; Runt-related transcription factor 1