Labdane Diterpenoids from Salvia leriifolia: Absolute Configuration, Antimicrobial and Cytotoxic Activities.
Fractionation of an n-hexane extract of the aerial parts of Salvia leriifolia led to the isolation of two new (1, 2) and two known (3, 4) labdane diterpenoids, together with three other known compounds. The structures were established by a combination of 1D and 2D NMR, and HRESIMS. The structures of 1 and 3 were confirmed by single-crystal X-ray analysis. The absolute configuration of 1-4 was established by electronic circular dichroism spectroscopy. Compounds 1-4 were evaluated for their cytotoxic activities against MCF-7 human breast cancer cells. Labdanes 3 and 4 were additionally tested against MDA-MB231 human breast cancer and DU-145 human prostate cancer cell lines. Compound 4 showed IC50 values of 25, 50, and 50 µM against MCF-7, MDA-MB231, and DU-145 cells, respectively. Compounds 1-4 were tested for activity against gram-positive (Staphylococcus aureus) and gram-negative (Escherichia coli) bacteria. Compound 3 showed an MIC of 213 µM against methicillin-resistant S. aureus.
Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G. C., Evin, Tehran, Iran.
Mahdi Moridi
MM
Farimani
Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G. C., Evin, Tehran, Iran.
Akram
A
Taleghani
Khorasan Razavi Agricultural and Natural Resources Research Center, Sabzevar Branch, Sabzevar, Khorasan Razavi, Iran.
Abbas
A
Aliabadi
Department of Biology, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G. C., Evin, Tehran, Iran.
Atousa
A
Aliahmadi
Department of Biology, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G. C., Evin, Tehran, Iran.
Mohammad Ali
MA
Esmaeili
Department of Animal Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran.
Nazanin
N
Namazi Sarvestani
Department of Chemistry, Faculty of Chemistry, Shahid Beheshti University, G. C., Evin, Tehran, Iran.
Hamid Reza
HR
Khavasi
Division of Molecular Modeling, University of Basel, Basel, Switzerland.
Martin
M
Smieško
Division of Pharmaceutical Biology, University of Basel, Basel, Switzerland.
Matthias
M
Hamburger
Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G. C., Evin, Tehran, Iran.
Samad
S
Nejad Ebrahimi
D000890:Anti-Infective Agents / Q000302:isolation & purification* / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D045744:Cell Line, Tumor; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D061986:MCF-7 Cells; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D027544:Salvia / Q000737:chemistry*
D000890:Anti-Infective Agents; D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes