Ampelopsis japonica Makino Extract Inhibits the Inflammatory Reaction Induced by Pathogen-Associated Molecular Patterns in Epidermal Keratinocytes.
BACKGROUND
Keratinocytes are the major cells in epidermis, providing barrier components such as cornified cells through the sophisticated differentiation process. In addition, keratinocytes exerts their role as the defense cells via activation of innate immunity. It has been known that pathogen-associated molecular patterns (PAMPs) including double-strand RNA and nucleotides can provoke inflammatory reaction in keratinocytes.


OBJECTIVE
The aim of this study is to evaluate the effect of Ampelopsis japonica Makino extract (AE) on PAMPs-induced inflammatory reaction of keratinocytes.


METHODS
The effects of AE were determined using poly (I:C)-induced inflammation and imiquimod-induced psoriasiform dermatitis models.


RESULTS
In cultured keratinocytes, AE significantly inhibited poly(I:C)-induced expression of inflammatory cytokines, such as interleukin (IL)-1β, IL-6, IL-8 and tumor necrosis factor-α. AE significantly inhibited poly(I:C)-induced release of caspase-1 active form (p20), and down-regulated nuclear factor-κB signaling pathway. In imiquimod-induced psoriasiform dermatitis model, topical application of AE resulted in significant reduction of epidermal hyperplasia.


CONCLUSIONS
These results suggest that AE may be a potential candidate for the treatment of skin inflammation.
Department of Dermatology, Chungnam National University School of Medicine, Daejeon, Korea.
Mi-Ra
MR
Choi
Department of Dermatology, Chungnam National University School of Medicine, Daejeon, Korea.
Dae-Kyoung
DK
Choi
Department of Dermatology, Chungnam National University School of Medicine, Daejeon, Korea.
Ki-Duck
KD
Kim
Department of Dermatology, Chungnam National University School of Medicine, Daejeon, Korea.
Sue Jeong
SJ
Kim
Department of Obstetrics and Gynecology, Dongguk University College of Korean Medicine, Seoul, Korea.
Dong-Il
DI
Kim
Department of Dermatology, Chungnam National University School of Medicine, Daejeon, Korea.
Myung
M
Im
Department of Dermatology, Chungnam National University School of Medicine, Daejeon, Korea.
Young
Y
Lee
Department of Dermatology, Chungnam National University School of Medicine, Daejeon, Korea.
Young-Joon
YJ
Seo
Department of Dermatology, Chungnam National University School of Medicine, Daejeon, Korea.
Chang Deok
CD
Kim
Department of Dermatology, Chungnam National University School of Medicine, Daejeon, Korea.; Skin Med Company, Daejeon, Korea.
Jeung-Hoon
JH
Lee
Ampelopsis; Inflammation; Keratinocytes; Pathogen-associated molecular pattern molecules