In Situ Assays of Chemotropism During Yeast Mating.
Virtually all eukaryotic cells can grow in a polarized fashion in response to external signals. Cells can respond to gradients of chemoattractants or chemorepellents by directional growth, a process referred to as chemotropism. The budding yeast Saccharomyces cerevisiae undergoes chemotropic growth during mating, in which two haploid cells of opposite mating type grow towards one another. Mating pheromone gradients are essential for efficient mating in yeast and different yeast mutants are defective in chemotropism. Two methods of assessing the ability of yeast strains to respond to pheromone gradients are presented here.
Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL, USA. dstone@uic.edu.
David E
DE
Stone
Institute of Biology Valrose, CNRS/INSERM/Université Nice-Sophia Antipolis, Valrose Parc, 06108, Nice Cedex 2, France. arkowitz@unice.fr.
Robert A
RA
Arkowitz
D000072235:Mating Factor / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D010641:Phenotype; D010675:Pheromones / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000302:isolation & purification* / Q000502:physiology*
D010675:Pheromones; D000072235:Mating Factor
Bud scar; Chemotropism; Gradient; Mating pheromone; Zygote