PEBP4 silencing inhibits hypoxia-induced epithelial-to-mesenchymal transition in prostate cancer cells.
Hypoxia induced epithelial-to-mesenchymal transition (EMT) to facilitate the tumor biology. Phosphatidylethanolamine-binding protein 4 (PEBP4) is a member of the PEBP family and has been reported to be upregulated in various cancer types. The definite function of PEBP4 in regulating the EMT of prostate cancer, however, is still unclear. Here, we examined the functional role of PEBP4 and the underlying molecular mechanisms in hypoxia-induced EMT in prostate cancer cells. Our results showed that PEBP4 mRNA and protein expression was markedly increased in the human prostate cancer tissues and cell lines. Knockdown of PEBP4 significantly inhibited hypoxia-induced migration/invasion and EMT program. Furthermore, knockdown of PEBP4 prevented hypoxia-induced the expression of p-Akt and p-mTOR in prostate cancer cells. Taken together, this study reported here provided evidence that knockdown of PEBP4 inhibited hypoxia-induced EMT in prostate cancer cells. Our study uncovered a novel role for PEBP4 in prostate cancer progression, which might support the potential for PEBP4 targeting in prostate cancer therapy.
Department of Urology, Xi'an Tangdu Hospital, the Fourth Military Medical University, Xi'an, Shaanxi 710038, China; Department of Urology, Lanzhou General Hospital of Lanzhou Military Region, Lanzhou, Gansu 730050, China.
Weiping
W
Li
Department of Urology, Lanzhou General Hospital of Lanzhou Military Region, Lanzhou, Gansu 730050, China.
Yongchao
Y
Dong
Department of Urology, Lanzhou General Hospital of Lanzhou Military Region, Lanzhou, Gansu 730050, China.
Bin
B
Zhang
Department of Urology, Lanzhou General Hospital of Lanzhou Military Region, Lanzhou, Gansu 730050, China.
Yindong
Y
Kang
Department of Urology, Lanzhou General Hospital of Lanzhou Military Region, Lanzhou, Gansu 730050, China.
Xukai
X
Yang
Department of Urology, Xi'an Tangdu Hospital, the Fourth Military Medical University, Xi'an, Shaanxi 710038, China. Electronic address: tdwanghe@sina.com.
He
H
Wang
D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D058750:Epithelial-Mesenchymal Transition; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D006801:Humans; D008297:Male; D009361:Neoplasm Invasiveness; D049488:Phosphatidylethanolamine Binding Protein / Q000235:genetics / Q000378:metabolism*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism
D049488:Phosphatidylethanolamine Binding Protein; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D058570:TOR Serine-Threonine Kinases; C518018:PE-binding protein 4, human
Epithelial-to-mesenchymal transition (EMT); Hypoxia; Phosphatidylethanolamine-binding protein 4 (PEBP4); Prostate cancer