In Vitro Interactions between Non-Steroidal Anti-Inflammatory Drugs and Antifungal Agents against Planktonic and Biofilm Forms of Trichosporon asahii.
Increasing drug resistance has brought enormous challenges to the management of Trichosporon spp. infections. The in vitro antifungal activities of non-steroidal anti-inflammatory drugs (NSAIDs) against Candida spp. and Cryptococcus spp. were recently discovered. In the present study, the in vitro interactions between three NSAIDs (aspirin, ibuprofen and diclofenac sodium) and commonly used antifungal agents (fluconazole, itraconazole, voriconazole, caspofungin and amphotericin B) against planktonic and biofilm cells of T. asahii were evaluated using the checkerboard microdilution method. The spectrophotometric method and the XTT reduction assay were used to generate data on biofilm cells. The fractional inhibitory concentration index (FICI) and the ΔE model were compared to interpret drug interactions. Using the FICI, the highest percentages of synergistic effects against planktonic cells (86.67%) and biofilm cells (73.33%) were found for amphotericin B/ibuprofen, and caspofungin/ibuprofen showed appreciable percentages (73.33% for planktonic form and 60.00% for biofilm) as well. We did not observe antagonism. The ΔE model gave consistent results with FICI (86.67%). Our findings suggest that amphotericin B/ibuprofen and caspofungin/ibuprofen combinations have potential effects against T. asahii. Further in vivo and animal studies to investigate associated mechanisms need to be conducted.
Department of Dermatology, General Hospital of Beijing Military Command, Beijing, China.|The Clinical Medical College in the Beijing Military Region, Second Military Medical University of People's Liberation Army, Shanghai, China.
Suteng
S
Yang
Department of Dermatology, General Hospital of Beijing Military Command, Beijing, China.|The Clinical Medical College in the Beijing Military Region, Second Military Medical University of People's Liberation Army, Shanghai, China.
Yong
Y
Liao
Department of Dermatology, General Hospital of Beijing Military Command, Beijing, China.
Lin
L
Cong
Department of Dermatology, General Hospital of Beijing Military Command, Beijing, China.
Xuelian
X
Lu
Department of Dermatology, General Hospital of Beijing Military Command, Beijing, China.
Rongya
http://orcid.org/0000-0002-4490-4007
R
Yang
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000819:agonists / Q000494:pharmacology*; D000935:Antifungal Agents / Q000493:pharmacokinetics*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D004357:Drug Synergism; D006801:Humans; D010933:Plankton / Q000502:physiology; D014250:Trichosporon / Q000502:physiology*; D060586:Trichosporonosis / Q000188:drug therapy
D000894:Anti-Inflammatory Agents, Non-Steroidal; D000935:Antifungal Agents