Hepatoprotective effects of Poly-[hemoglobin-superoxide dismutase-catalase-carbonic anhydrase] on alcohol-damaged primary rat hepatocyte culture in vitro.
We have prepared a novel nanobiotherapeutic, Poly-[hemoglobin-superoxide dismutase-catalase-carbonic anhydrase], which not only transports both oxygen and carbon dioxide but also a therapeutic antioxidant. Our previous study in a severe sustained 90 min hemorrhagic shock rat model shows that it has a hepatoprotective effect. We investigate its hepatoprotective effect further in this present report using an alcohol-damaged primary hepatocyte culture model. Results show that it significantly reduced ethanol-induced AST release, lipid peroxidation, and ROS production in rat primary hepatocytes culture. It also significantly enhanced the viability of ethanol-treated hepatocytes. Thus, the result shows that Poly-[hemoglobin-superoxide dismutase-catalase-carbonic anhydrase] also has some hepatoprotective effects against alcohol-induced injury in in vitro rat primary hepatocytes cell culture. This collaborate our previous observation of its hepatoprotective effect in a severe sustained 90-min hemorrhagic shock rat model.
a Departments of Physiology, Medicine and Biomedical Engineering, Faculty of Medicine , Artificial Cells and Organs Research Centre, McGill University , Montreal , QC , Canada.
Wenhua
W
Jiang
a Departments of Physiology, Medicine and Biomedical Engineering, Faculty of Medicine , Artificial Cells and Organs Research Centre, McGill University , Montreal , QC , Canada.
Yuzhu
Y
Bian
a Departments of Physiology, Medicine and Biomedical Engineering, Faculty of Medicine , Artificial Cells and Organs Research Centre, McGill University , Montreal , QC , Canada.
Zhenghui
Z
Wang
a Departments of Physiology, Medicine and Biomedical Engineering, Faculty of Medicine , Artificial Cells and Organs Research Centre, McGill University , Montreal , QC , Canada.
Thomas Ming Swi
TM
Chang
D000818:Animals; D002256:Carbonic Anhydrases / Q000737:chemistry / Q000494:pharmacology; D002374:Catalase / Q000737:chemistry / Q000494:pharmacology; D002417:Cattle; D002478:Cells, Cultured; D000431:Ethanol / Q000633:toxicity*; D006454:Hemoglobins / Q000737:chemistry / Q000494:pharmacology; D022781:Hepatocytes / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D012771:Shock, Hemorrhagic / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D013482:Superoxide Dismutase / Q000737:chemistry / Q000494:pharmacology
D006454:Hemoglobins; D000431:Ethanol; D002374:Catalase; D013482:Superoxide Dismutase; D002256:Carbonic Anhydrases
Alcohol; PolyHb; aspartate aminotransferase; hepatoprotective; malondialdehyde; polyhemoglobin-superoxide dismutase-catalase-carbonic anhydrase; primary rat hepatocytes; reactive oxygen species