In vitro and in vivo targeting imaging of pancreatic cancer using a Fe3O4@SiO2 nanoprobe modified with anti-mesothelin antibody.
Pancreatic cancer is a highly malignant disease with a 5-year survival rate <5% mainly due to lack of early diagnosis and effective therapy. In an effort to improve the early diagnostic rate of pancreatic cancer, a nanoprobe Fe3O4@SiO2 modified with anti-mesothelin antibody (A-MFS) was prepared to target cells and tumor tissues highly expressing mesothelin in vitro (human pancreatic cancer cell line SW1990) and in vivo (subcutaneously transplanted tumors) studies. The A-MFS probe was successfully prepared and was spherical and uniform with a hydrodynamic diameter between 110 and 130 nm. Cell Counting Kit-8 testing indicated that A-MFS was nontoxic in vitro and in vivo studies. The in vitro study showed that the A-MFS probe specifically targeted SW1990 cells with high mesothelin expression. The in vivo study was conducted in Siemens 3.0 T magnetic resonance imaging. The average T2-weighted signal values of the xenografts were 966.533±31.56 before injecting A-MFS and 691.133±56.84 before injecting saline solution. After injection of 0.1 mL A-MFS via nude mouse caudal vein for 2.5 hours, the average T2-weighted signal of the xenograft decreased by 342.533±42.6. The signal value decreased by -61.233±33.9 and -58.7±19.4 after injection of the saline and Fe3O4@SiO2. The decrease of tumor signal by A-MFS was much more significant than that by saline and Fe3O4@SiO2 (P<0.05). The results demonstrated the high stability and nontoxicity of A-MFS, which effectively targeted pancreatic cancer in vitro and in vivo. A-MFS is a promising agent for diagnosis of pancreatic cancer.
Radiology Department of Changhai Hospital, Second Military Medical University, Shanghai, People's Republic of China.
Fang
F
Liu
Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science, Tongji University School of Medicine, Shanghai, People's Republic of China.
Wenjun
W
Le
Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science, Tongji University School of Medicine, Shanghai, People's Republic of China.
Tianxiao
T
Mei
Radiology Department of Changhai Hospital, Second Military Medical University, Shanghai, People's Republic of China.
Tiegong
T
Wang
Radiology Department of Changhai Hospital, Second Military Medical University, Shanghai, People's Republic of China.
Luguang
L
Chen
Radiology Department of Changhai Hospital, Second Military Medical University, Shanghai, People's Republic of China.
Yi
Y
Lei
Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science, Tongji University School of Medicine, Shanghai, People's Republic of China.
Shaobin
S
Cui
Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science, Tongji University School of Medicine, Shanghai, People's Republic of China.
Bingdi
B
Chen
Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science, Tongji University School of Medicine, Shanghai, People's Republic of China; Department of Pathology, Wake Forest University School of Medicine, Winston-Salem, NC, USA.
Zheng
Z
Cui
Radiology Department of Changhai Hospital, Second Military Medical University, Shanghai, People's Republic of China.
Chengwei
C
Shao
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003911:Dextrans / Q000737:chemistry*; D016503:Drug Delivery Systems; D005434:Flow Cytometry; D058851:GPI-Linked Proteins / Q000276:immunology*; D006801:Humans; D007501:Iron / Q000378:metabolism; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008297:Male; D000090204:Mesothelin; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010190:Pancreatic Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D012822:Silicon Dioxide / Q000737:chemistry*
D000906:Antibodies; D003911:Dextrans; D058851:GPI-Linked Proteins; D058185:Magnetite Nanoparticles; C000715528:Msln protein, mouse; D012822:Silicon Dioxide; D007501:Iron; C065507:ferumoxides; D000090204:Mesothelin
Fe3O4@SiO2; mesothelin; nanoprobe; pancreatic cancer; targeted imaging