Comprehensive comparison of bacterial communities in a membrane-free bioelectrochemical system for removing different mononitrophenols from wastewater.
Membrane-free bioelectrochemical systems (MFBESs) have been developed for the degradation of nitro-aromatic contaminants, but the microbial communities that are involved have not been comprehensively investigated. In this study, the microbial communities were evaluated and compared for treating different structures of nitrophenols (NPs), i.e., o-nitrophenol (ONP), m-nitrophenol (MNP) and p-nitrophenol (PNP), in the MFBES. The results demonstrated that NPs reduction in the MFBES decreased in efficiency in the following order: ONP>MNP>PNP. Illumina MiSeq sequencing results showed that richness and diversity of bacterial species in the anodic and cathodic communities decreased when fed different NPs. Though remarkable differences in community composition were found between anodic and cathodic biofilms in the MFBES, three core genera-Treponema, Desulfovibrio and Geobacter-were dominant in the anodic or cathodic biofilm, regardless of various NPs. Other functional genera in the anodic or cathodic biofilm were selectively enriched in the MFBES treating the three NPs with different structures.
Jiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, Jiangsu Province, China.
Xinbai
X
Jiang
Jiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, Jiangsu Province, China. Electronic address: shenjinyou@mail.njust.edu.cn.
Jinyou
J
Shen
Jiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, Jiangsu Province, China; Jiangsu Radiation Environment Protection Consultation Center, Nanjing 210019, Jiangsu Province, China.
Shuai
S
Lou
CAS Key Laboratory of Urban Pollutant Conversion, Collaborative Innovation Centre of Suzhou Nano Science and Technology, Department of Chemistry, University of Science and Technology of China, Hefei 230026, Anhui Province, China.
Yang
Y
Mu
Jiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, Jiangsu Province, China.
Ning
N
Wang
Jiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, Jiangsu Province, China.
Weiqing
W
Han
Jiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, Jiangsu Province, China.
Xiuyun
X
Sun
Jiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, Jiangsu Province, China.
Jiansheng
J
Li
Jiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, Jiangsu Province, China.
Lianjun
L
Wang
D001674:Bioelectric Energy Sources / Q000382:microbiology; D018441:Biofilms; D019149:Bioreactors; D003901:Desulfovibrio / Q000235:genetics; D055664:Electrochemical Techniques; D045302:Geobacter / Q000235:genetics; D059013:Microbial Consortia / Q000235:genetics; D058889:Molecular Typing; D009596:Nitrophenols / Q000737:chemistry / Q000302:isolation & purification*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017423:Sequence Analysis, RNA; D012722:Sewage / Q000382:microbiology; D014208:Treponema / Q000235:genetics; D062065:Wastewater / Q000032:analysis / Q000737:chemistry*; D018508:Water Purification
D009596:Nitrophenols; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D062065:Wastewater
Bioelectrochemical system; Microbial community; Nitrophenol; Reduction