Low cost and compact analytical microsystem for carbon dioxide determination in production processes of wine and beer.
The design, construction and evaluation of a low cost, cyclic olefin copolymer (COC)-based continuous flow microanalyzer, with optical detection, to monitor carbon dioxide in bottled wines and beers as well as in fermentation processes, is presented. The microsystem, constructed by computer numerically controlled (CNC) micromilling and using a multilayer approach, integrates microfluidics, gas-diffusion module and an optical flow-cell in a single polymeric substrate. Its size is slightly bigger than a credit card, exactly 45 × 60 × 4 mm in the microfluidic and diffusion module zone and 22.5 × 40 × 3 mm in the flow-cell zone. The gas-diffusion module is based on a hydrophobic polyvinylidene fluoride (PVDF) membrane, which allows the transfer of the carbon dioxide present in the sample to a bromothymol blue (BTB) pH-sensitive acceptor solution, where the color change is measured optically. The detection system consisted of a LED with an emission peak at 607 nm and a photodiode integrated in a printed circuit board (PCB). The obtained analytical features after the optimization of the microfluidic platform and hydrodynamic variables are a linear range from 255 to 10000 mg L(-1) of CO2 and a detection limit of 83 mg L(-1) with a sampling rate of 30 samples h(-1).
Group of Sensors and Biosensors, Department of Chemistry, Autonomous University of Barcelona, Edifici Cn, 08193 Barcelona, Spain.
Antonio
A
Calvo-López
Group of Sensors and Biosensors, Department of Chemistry, Autonomous University of Barcelona, Edifici Cn, 08193 Barcelona, Spain.
Oriol
O
Ymbern
Centro Universitario de la Defensa (CUD), Academia General Militar, Carretera de Huesca s/n, Zaragoza, 50090, Spain; Grupo de Tecnologías Fotónicas (GTF), Aragon Institute of Engineering Research (I3A), Universidad de Zaragoza, Mariano Esquillor ed. I+D+i, Zaragoza, 50018, Spain.
David
D
Izquierdo
Group of Sensors and Biosensors, Department of Chemistry, Autonomous University of Barcelona, Edifici Cn, 08193 Barcelona, Spain. Electronic address: Julian.Alonso@uab.es.
Julián
J
Alonso-Chamarro
D001515:Beer / Q000032:analysis*; D001979:Bromthymol Blue / Q000737:chemistry; D002245:Carbon Dioxide / Q000032:analysis*; D003516:Cycloparaffins / Q000737:chemistry; D004058:Diffusion; D005740:Gases / Q000737:chemistry; D057230:Limit of Detection; D044085:Microfluidics / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D008904:Miniaturization; D011108:Polymers / Q000737:chemistry; D011145:Polyvinyls / Q000737:chemistry; D014920:Wine / Q000032:analysis*; D005465:Fluorocarbon Polymers
D001979:Bromthymol Blue; D002245:Carbon Dioxide; D003516:Cycloparaffins; D005740:Gases; D011108:Polymers; D011145:Polyvinyls; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers
Carbon dioxide; Cyclic olefin co-polymer; Gas-diffusion; Lab on a chip; Miniaturization; Optical detection