miR-200a-3p regulates TLR1 expression in bacterial challenged miiuy croaker.
MicroRNAs (miRNAs) are highly conserved, small non-coding RNAs which post-transcriptionally regulate various biological processes by repressing mRNA translation or degradating mRNA. It has been demonstrated that miRNAs play crucial roles in regulating the immune system. In this study, we explored the potential roles of miR-200a-3p in regulating TLR signaling pathway in miiuy croaker. Bioinformatics analysis showed that miiuy croaker TLR1 (mmiTLR1) was a putative target of miR-200a-3p. Negative expression profiles in spleen of Vibrio anguillarum challenged miiuy croaker and in lipopolysaccharide (LPS) stimulated miiuy croaker leukocytes further validated the prediction. Luciferase reporter assays showed that the dual-luciferase reporter fused to the 3'UTR of wild type mmiTLR1 cotransfected with miR-200a-3p mimics exhibited a reduction in luciferase activity compared with the controls. All of the present data provide direct evidence that miR-200a-3p is involved in TLR1 expression modulation in miiuy croaker, which will offer a basis for better understanding of miRNA regulation in fish TLR signaling pathways.
Laboratory of Fish Biogenetics & Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316022, China.
Yanjin
Y
Wang
Laboratory of Fish Biogenetics & Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316022, China.
Guoliang
G
Xu
Laboratory of Fish Biogenetics & Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316022, China.
Jingjing
J
Han
Laboratory of Fish Biogenetics & Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan, 316022, China. Electronic address: tianjunxu@163.com.
Tianjun
T
Xu
D000818:Animals; D002460:Cell Line; D019295:Computational Biology; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D056747:Immunomodulation; D007962:Leukocytes / Q000276:immunology*; D035683:MicroRNAs / Q000235:genetics*; D010473:Perciformes / Q000276:immunology*; D013154:Spleen / Q000502:physiology*; D051194:Toll-Like Receptor 1 / Q000235:genetics / Q000378:metabolism*
D029941:Fish Proteins; C533896:MIRN200 microRNA, human; D035683:MicroRNAs; D051194:Toll-Like Receptor 1
Miiuy croaker; Regulation; Signaling pathway; TLR1; Toll-like receptor; miR-200a-3p