Ras-Related Nuclear Protein Ran3B Gene Is Involved in Hormone Responses in the Embryogenic Callus of Dimocarpus longan Lour.
Ras-related guanosine triphosphate (GTP)-binding nuclear protein (Ran) GTPases function as molecular switches and regulate diverse cellular events in eukaryotes. Our previous work suggested that DlRan3B is active during longan (Dimocarpus longan Lour.) somatic embryogenesis (SE) processes. Herein, subcellular localization of DlRan3B was found to be localized in the nucleus and expression profiling of DlRan3B was performed during longan SE and after exposure to plant hormones (indoleacetic acid (IAA), gibberellin A3 (GA₃), salicylic acid (SA), methyl jasmonte (MeJA), and abscisic acid (ABA)). We cloned and sequenced 1569 bp of 5'-flanking sequence of DlRan3B (GenBank: JQ279697). Bioinformatic analysis indicated that the promoter contained plant hormone-related regulatory elements. Deletion analysis and responses to hormones identified stimulative and repressive regulatory elements in the DlRan3B promoter. The key elements included those responding to auxin, gibberellin, SA, MeJA, and ABA. DlRan3B was located in the nucleus and accumulated in the late stage of longan SE. The expression of DlRan3B was significantly induced by IAA, GA₃, and ABA, but suppressed by SA and MeJA. Promoter transcription was induced by IAA and GA₃, but suppressed by SA. Thus, DlRan3B might participate in auxin, gibberellin, and ABA responses during longan late SE, and DlRan3B is involved in phytohormone responsiveness.
Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China. tianqilin2009@163.com.
Qilin
Q
Tian
Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China. buliang84@163.com.
Yuling
Y
Lin
Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China. qiandanqianlan2011@163.com.
Dongmin
D
Zhang
Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China. lairl0618@163.com.
Ruilian
R
Lai
Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China. laizx01@163.com.
Zhongxiong
Z
Lai
D001483:Base Sequence; D019295:Computational Biology / Q000379:methods; D047108:Embryonic Development / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D010937:Plant Growth Regulators / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D021381:Protein Transport; D012045:Regulatory Sequences, Nucleic Acid; D029631:Sapindaceae / Q000196:embryology / Q000235:genetics* / Q000378:metabolism*; D017384:Sequence Deletion
D010937:Plant Growth Regulators; D010940:Plant Proteins
DlRan3B; Ran GTPases; SE; gene expression; promoter deletion analysis