Visualizing Cancer Cell Chemotaxis and Invasion in 2D and 3D.
We describe three chemotaxis assays-Insall chambers, circular invasion assays, and 3D organotypic assays-that are particularly appropriate for measuring migration of cancer cells in response to gradients of soluble attractants. Each assay has defined advantages, and together they provide the best possible quantitative assessment of cancer chemotaxis.
CR-UK Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK.
Olivia
O
Susanto
Melanoma Clinic, Leeds Cancer Centre, St. James's University Hospital, Beckett Street, Leeds, LS9 7TF, UK.
Andrew J
AJ
Muinonen-Martin
CR-UK Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK.
Max
M
Nobis
CR-UK Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK. r.insall@beatson.gla.ac.uk.
Robert H
RH
Insall
D000818:Animals; D018929:Cell Culture Techniques; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D002465:Cell Movement; D002633:Chemotaxis; D006801:Humans; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology*; D051381:Rats; D014407:Tumor Cells, Cultured
Cell migration; Chemotaxis; Invasion; Melanoma; Metastasis; Organotypic