Effects of simulated microgravity on human brain nervous tissue.
During spaceflight, the negative effects of space microgravity on astronauts are becoming more and more prominent, and especially, of which on the nervous system is urgently to be solved. For this purpose tissue blocks and primary cells of nervous tissues obtained from glioma of patients were cultivated after culturing for about 7days, explanted tissues and cells were then randomly divided into two groups, one for static culture (control group, C), and the other for rotary processing for 1day, 3days, 5days, 7days and 14days (experiment group, E). Figures captured by inverted microscope revealed that, with short time rotating for 1day or 3days, morphology changes of tissue blocks were not obvious. When the rotary time was extended to 7days or 14days, it was found that cell somas is significantly larger and the ability of adhesion is declined in comparison with that in control group. Additionally, the arrangement of cells migrated from explanted tissues was disorganized, and the migration distance became shorter. In immunofluorescence analysis, β-tubulin filaments in control group appeared to organize into bundles. While in experiment group, β-tubulin was highly disorganized. In conclusion, simulated microgravity treatment for a week affected the morphology of nervous tissue, and caused highly disorganized distribution of cytoskeleton and the increase of cell apoptosis. These morphological changes might be one of the causes of apoptosis induced by simulated microgravity.
School of Life Science, Beijing Institute of Technology, Beijing 100081, China. Electronic address: wxhan1986@163.com.
Xianghan
X
Wang
Xuanwu Hospital, Capital Medical University, Beijing 100053, China. Electronic address: 13001285670@wo.cn.
Jianxin
J
Du
School of Life Science, Beijing Institute of Technology, Beijing 100081, China.
Demei
D
Wang
School of Life Science, Beijing Institute of Technology, Beijing 100081, China.
Fan
F
Zeng
Xuanwu Hospital, Capital Medical University, Beijing 100053, China.
Yukui
Y
Wei
School of Life Science, Beijing Institute of Technology, Beijing 100081, China.
Fuli
F
Wang
School of Life Science, Beijing Institute of Technology, Beijing 100081, China.
Chengcheng
C
Feng
School of Life Science, Beijing Institute of Technology, Beijing 100081, China.
Nuomin
N
Li
School of Life Science, Beijing Institute of Technology, Beijing 100081, China.
Rongji
R
Dai
School of Life Science, Beijing Institute of Technology, Beijing 100081, China.
Yulin
Y
Deng
School of Life Science, Beijing Institute of Technology, Beijing 100081, China. Electronic address: qzzbit2015@bit.edu.cn.
Zhenzhen
Z
Quan
School of Life Science, Beijing Institute of Technology, Beijing 100081, China. Electronic address: hqing@bit.edu.cn.
Hong
H
Qing
D017209:Apoptosis; D001921:Brain / Q000473:pathology*; D002465:Cell Movement; D003599:Cytoskeleton / Q000473:pathology; D006801:Humans; D009457:Neuroglia / Q000473:pathology; D009474:Neurons / Q000473:pathology; D014407:Tumor Cells, Cultured; D018474:Weightlessness Simulation / Q000009:adverse effects*
Apoptosis; Cytoskeleton; Microgravity; Morphology; Nervous tissue