Hybrid biomaterials based on calcium carbonate and polyaniline nanoparticles for application in photothermal therapy.
Inorganic materials contain remarkable properties for drug delivery, such as a large surface area and nanoporous structure. Among these materials, CaCO3 microparticles (CMPs) exhibit a high encapsulation efficiency and solubility in acidic media. The extracellular pH of tumor neoplastic tissue is significantly lower than the extracellular pH of normal tissue facilitating the release of drug-encapsulating CMPs in this area. Conducting polyaniline (PANI) absorbs light energy and transforms it into localized heat to produce cell death. This work aimed to generate hybrid CMPs loaded with PANI for photothermal therapy (PTT). The hybrid nanomaterial was synthesized with CaCO3 and carboxymethyl cellulose in a simple, reproducible manner. The CMP-PANI-Cys particles were developed for the first time and represent a novel type of hybrid biomaterial. Resultant nanoparticles were characterized utilizing scanning electron microscopy, dynamic light scattering, zeta potential, UV-vis, FTIR and Raman spectroscopy. In vitro HeLa cells in dark and irradiated conditions showed that CMP-PANI-Cys and PANI-Cys are nontoxic at the assayed concentrations. Hybrid biomaterials displayed high efficiency for potential PTT compared with PANI-Cys. In summary, hierarchical hybrid biomaterials composed of CMPs and PANI-Cys combined with near infrared irradiation represents a useful alternative in PTT.
Departamento de Ciencias Biológicas Animales, Fac. de Cs. Veterinarias y Pecuarias, Universidad de Chile, Av. Santa Rosa 11735, La Pintana, Santiago, 8820000, Chile; Centro Avanzado de Enfermedades Crónicas (ACCDiS), Universidad de Chile, Sergio Livingstone 1007, Independencia, Santiago, 8380000, Chile. Electronic address: aneira@uchile.cl.
Andrónico
A
Neira-Carrillo
Departamento de Ciencias Biológicas Animales, Fac. de Cs. Veterinarias y Pecuarias, Universidad de Chile, Av. Santa Rosa 11735, La Pintana, Santiago, 8820000, Chile; Departamento Biología Molecular, Fac. CEF-Q y N, Universidad Nacional de Río Cuarto, CONICET, Ruta 36 km 601, Río Cuarto Cordoba, 5800, Argentina; Departamento de Química Farmacológica y Toxicológica, Fac. Cs. Químicas y Farmacéuticas, Universidad de Chile. Sergio Livingstone 1007, Independencia, Santiago, 8380000, Chile.
Edith
E
Yslas
Departamento de Ciencias Biológicas Animales, Fac. de Cs. Veterinarias y Pecuarias, Universidad de Chile, Av. Santa Rosa 11735, La Pintana, Santiago, 8820000, Chile; Centro Avanzado de Enfermedades Crónicas (ACCDiS), Universidad de Chile, Sergio Livingstone 1007, Independencia, Santiago, 8380000, Chile.
Yazmin Amar
YA
Marini
Departamento de Ciencias Biológicas Animales, Fac. de Cs. Veterinarias y Pecuarias, Universidad de Chile, Av. Santa Rosa 11735, La Pintana, Santiago, 8820000, Chile; Centro Avanzado de Enfermedades Crónicas (ACCDiS), Universidad de Chile, Sergio Livingstone 1007, Independencia, Santiago, 8380000, Chile.
Patricio
P
Vásquez-Quitral
Departamento de Ciencias Biológicas Animales, Fac. de Cs. Veterinarias y Pecuarias, Universidad de Chile, Av. Santa Rosa 11735, La Pintana, Santiago, 8820000, Chile; Centro Avanzado de Enfermedades Crónicas (ACCDiS), Universidad de Chile, Sergio Livingstone 1007, Independencia, Santiago, 8380000, Chile.
Marianela
M
Sánchez
Centro Avanzado de Enfermedades Crónicas (ACCDiS), Universidad de Chile, Sergio Livingstone 1007, Independencia, Santiago, 8380000, Chile; Departamento de Química Farmacológica y Toxicológica, Fac. Cs. Químicas y Farmacéuticas, Universidad de Chile. Sergio Livingstone 1007, Independencia, Santiago, 8380000, Chile.
Ana
A
Riveros
Departamento de Ciencias Biológicas Animales, Fac. de Cs. Veterinarias y Pecuarias, Universidad de Chile, Av. Santa Rosa 11735, La Pintana, Santiago, 8820000, Chile; Centro Avanzado de Enfermedades Crónicas (ACCDiS), Universidad de Chile, Sergio Livingstone 1007, Independencia, Santiago, 8380000, Chile.
Diego
D
Yáñez
Departamento de Química CONICET y Tecnología Química, Universidad Nacional de Río Cuarto. Ruta 36km 601, Río Cuarto, Córdoba, 5800, Argentina.
Pablo
P
Cavallo
Centro Avanzado de Enfermedades Crónicas (ACCDiS), Universidad de Chile, Sergio Livingstone 1007, Independencia, Santiago, 8380000, Chile; Departamento de Química Farmacológica y Toxicológica, Fac. Cs. Químicas y Farmacéuticas, Universidad de Chile. Sergio Livingstone 1007, Independencia, Santiago, 8380000, Chile.
Marcelo J
MJ
Kogan
Departamento de Química CONICET y Tecnología Química, Universidad Nacional de Río Cuarto. Ruta 36km 601, Río Cuarto, Córdoba, 5800, Argentina. Electronic address: dacevedo@exa.unrc.edu.ar.
Diego
D
Acevedo
D000814:Aniline Compounds / Q000737:chemistry*; D001672:Biocompatible Materials / Q000494:pharmacology*; D002119:Calcium Carbonate / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D003545:Cysteine / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D006979:Hyperthermia, Induced / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D010789:Phototherapy / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D055672:Static Electricity
D000814:Aniline Compounds; D001672:Biocompatible Materials; C416807:polyaniline; D002119:Calcium Carbonate; D003545:Cysteine
Biological applications of polymers; Biomineralization; Drug delivery systems; Nanocomposites; Nanoparticles