Performance of a PCR assay for the rapid identification of the Klebsiella pneumoniae ST258 epidemic clone in Latin American clinical isolates.
The worldwide dissemination of Klebsiella pneumoniae carbapenemase (KPC)-producing Klebsiella pneumoniae ST258 demands a rapid PCR-based typing method to detect unique genes of the ST258 clone. This study evaluates a PCR developed by Adler et al. (2014) for the detection of ST258 in K. pneumoniae clinical isolates centered on the identification of the pilv-I and prp genes. We tested 143 clinical isolates from Argentina (n=109), Chile (n=1), Colombia (n=1), Costa Rica (n=2), Ecuador (n=5), El Salvador (n=2), Nicaragua (n=5), Panamá (n=2), Paraguay (n=2), Perú (n=3) and Trinidad and Tobago (n=11) recovered from 2006 to 2015. blaKPC, pilv-l and prp genes were detected by PCR and sequenced by standard procedures. ST258 and non-ST258 were defined by PFGE and/or MLST. Isolates were grouped according to PFGE patterns: 58 were compatible with ST258 (group 1) and 85 with non-ST258 (group 2). MLST study was done on an arbitrary selection of isolates. The pilv-l gene was present only in ST258 isolates, regardless of the presence of the blaKPC gene. Results for the prp gene were variable. Its presence did not define ST258. The pilv-I PCR had a sensitivity and specificity of 100%, respectively, for the detection of ST258 in the isolates under investigation. Given our findings, the pilv-I PCR could replace more time and resource consuming methods, allowing for more rapid detection of the circulating high risk K. pneumoniae clone ST258 in Latin American (LA) countries.
Servicio Antimicrobianos, Laboratorio Regional de Referencia en Resistencia a los Antimicrobianos (LRR), INEI-ANLIS "Dr. Carlos G. Malbrán", Buenos Aires, Argentina; Research Career, CONICET (Consejo Nacional de Investigaciones Científicas y Tecnológicas), Buenos Aires, Argentina. Electronic address: sgomez@anlis.gov.ar.
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Gomez
Servicio Antimicrobianos, Laboratorio Regional de Referencia en Resistencia a los Antimicrobianos (LRR), INEI-ANLIS "Dr. Carlos G. Malbrán", Buenos Aires, Argentina.
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Rapoport
Servicio Antimicrobianos, Laboratorio Regional de Referencia en Resistencia a los Antimicrobianos (LRR), INEI-ANLIS "Dr. Carlos G. Malbrán", Buenos Aires, Argentina.
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Piergrossi
Servicio Antimicrobianos, Laboratorio Regional de Referencia en Resistencia a los Antimicrobianos (LRR), INEI-ANLIS "Dr. Carlos G. Malbrán", Buenos Aires, Argentina; Research Career, CONICET (Consejo Nacional de Investigaciones Científicas y Tecnológicas), Buenos Aires, Argentina.
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Faccone
Servicio Antimicrobianos, Laboratorio Regional de Referencia en Resistencia a los Antimicrobianos (LRR), INEI-ANLIS "Dr. Carlos G. Malbrán", Buenos Aires, Argentina.
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Pasteran
Servicio Antimicrobianos, Laboratorio Regional de Referencia en Resistencia a los Antimicrobianos (LRR), INEI-ANLIS "Dr. Carlos G. Malbrán", Buenos Aires, Argentina; Research Career, CONICET (Consejo Nacional de Investigaciones Científicas y Tecnológicas), Buenos Aires, Argentina.
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De Belder
Red Latinoamericana de Vigilancia de la Resistencia a los Antimicrobianos (ReLAVRA).
ReLAVRA-Group
Servicio Antimicrobianos, Laboratorio Regional de Referencia en Resistencia a los Antimicrobianos (LRR), INEI-ANLIS "Dr. Carlos G. Malbrán", Buenos Aires, Argentina.
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Petroni
Servicio Antimicrobianos, Laboratorio Regional de Referencia en Resistencia a los Antimicrobianos (LRR), INEI-ANLIS "Dr. Carlos G. Malbrán", Buenos Aires, Argentina.
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Corso
D001426:Bacterial Proteins / Q000235:genetics; D006801:Humans; D007710:Klebsiella Infections / Q000453:epidemiology / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D013020:South America / Q000453:epidemiology; D001618:beta-Lactamases / Q000235:genetics
D001426:Bacterial Proteins; C077573:PilV protein, Bacteria; D001618:beta-Lactamases; C063912:carbapenemase
Klebsiella pneumoniae; ST258 detection; bla(KPC); pilv-l; prp