Transport mechanism of a glutamate transporter homologue GltPh.
Glutamate transporters are responsible for uptake of the neurotransmitter glutamate in mammalian central nervous systems. Their archaeal homologue GltPh, an aspartate transporter isolated from Pyrococcus horikoshii, has been the focus of extensive studies through crystallography, MD simulations and single-molecule FRET (smFRET). Here, we summarize the recent research progress on GltPh, in the hope of gaining some insights into the transport mechanism of this aspartate transporter.
College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China Astbury Centre for Structural Molecular Biology, School of Biomedical Sciences, University of Leeds, Leeds, LS2 9JT, U.K.
Yurui
Y
Ji
Astbury Centre for Structural Molecular Biology, School of Biomedical Sciences, University of Leeds, Leeds, LS2 9JT, U.K. Biomedicine Research Group, Faculty of Health and Social Sciences, Leeds Beckett University, Leeds, LS1 3JT, U.K.
Vincent L G
VL
Postis
College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China.
Yingying
Y
Wang
Astbury Centre for Structural Molecular Biology, School of Biomedical Sciences, University of Leeds, Leeds, LS2 9JT, U.K. College of Life Sciences, Nankai University, Tianjin 300071, China bartlam@nankai.edu.cn a.goldman@leeds.ac.uk.
Mark
M
Bartlam
Astbury Centre for Structural Molecular Biology, School of Biomedical Sciences, University of Leeds, Leeds, LS2 9JT, U.K. College of Life Sciences, Nankai University, Tianjin 300071, China Division of Biochemistry, Department of Biosciences, University of Helsinki, Helsinki, FIN-00014, Finland bartlam@nankai.edu.cn a.goldman@leeds.ac.uk.
Adrian
A
Goldman
D027322:Amino Acid Transport System X-AG / Q000378:metabolism*; D001224:Aspartic Acid / Q000378:metabolism; D001692:Biological Transport; D011487:Protein Conformation; D043902:Pyrococcus horikoshii / Q000378:metabolism*; D013379:Substrate Specificity
D027322:Amino Acid Transport System X-AG; D001224:Aspartic Acid
Cl− conductance; GltPh; Na+ coupling; aspartate transporter; excitatory amino acid transporters (EAATs); glutamate transporter