Use of MicroRNA biomarkers to distinguish enchondroma from low-grade chondrosarcoma.
Establishing a definitive diagnosis between benign enchondroma versus low-grade chondrosarcoma presents a potential challenge to both clinicians and pathologists. microRNAs (small non-coding RNAs) have proven to be effective biomarkers for the identification of tumors and tumor progression. We present analysis, both array and quantitative PCR, that shows consistently and substantially increased expression of two microRNAs, miRs-181a and -138, in low-grade chondrosarcomas compared with enchondromas. The data suggest these microRNAs would provide an analytical distinction between the chondrosarcoma and benign neoplasms that can be performed in formalin-fixed paraffin-embedded specimens. Together with recent publications, these data indicate that miRs-181a and -138 also play a role in tumor development and homeostasis and may provide new targets for the development of much needed therapeutic intervention.
a Bone and Joint Center , Henry Ford Hospital , Detroit , MI , USA.
Liang
L
Zhang
a Bone and Joint Center , Henry Ford Hospital , Detroit , MI , USA.
Maozhou
M
Yang
b Department of Pathology , Henry Ford Hospital , Detroit , MI , USA.
Theodore
T
Mayer
c Department of Orthopaedics and Rehabilitation , Oregon Health and Science University , Portland , OR , USA.
Brian
B
Johnstone
a Bone and Joint Center , Henry Ford Hospital , Detroit , MI , USA.
Clifford
C
Les
d Department of Orthopaedic Surgery , Henry Ford Hospital , Detroit , MI , USA.
Nicholas
N
Frisch
d Department of Orthopaedic Surgery , Henry Ford Hospital , Detroit , MI , USA.
Theodore
T
Parsons
e Department of Dermatology , Henry Ford Hospital , Detroit , MI , USA.
Qing-Sheng
QS
Mi
a Bone and Joint Center , Henry Ford Hospital , Detroit , MI , USA.
Gary
G
Gibson
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000235:genetics; D002812:Chondroma / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002813:Chondrosarcoma / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics; D008875:Middle Aged; D012334:RNA, Neoplasm / Q000096:biosynthesis / Q000235:genetics
D014408:Biomarkers, Tumor; C546110:MIRN138 microRNA, human; C540193:MIrn181 microRNA, human; D035683:MicroRNAs; D012334:RNA, Neoplasm
chondrosarcoma; enchondroma; miR-138; miR-181a; microRNA