Deparaffinization of formalin-fixed paraffin-embedded tissue blocks using hot water instead of xylene.
This study aimed to deparaffinize formalin-fixed paraffin-embedded (FFPE) tissues using hot water instead of xylene and measuring the quantity and quality of the extracted DNA from the respective tissues. To deparaffinize the tissue sections with hot water, small sections were exposed to 90 °C distilled sterile water. After 25 FFPE tissue samples were deparaffinized with the hot water method, DNA was then extracted. The mean of optical density and the ratio of absorbance of the DNA solution were 220.01 ± 36.1 ng/μl and 1.65 ± 0.1, respectively. Polymerase chain reaction (PCR) analysis of the toll-like receptor 4(TLR4) gene showed that the method can be used as a tool for different applications.
Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
Narges
N
Kalantari
Infectious Diseases and Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran. Electronic address: m_baiany@yahoo.com.
Masomeh
M
Bayani
Student Research Committee, Babol University of Medical Sciences, Babol, Iran.
Taraneh
T
Ghaffari
D004247:DNA / Q000032:analysis / Q000737:chemistry; D005557:Formaldehyde / Q000737:chemistry*; D006358:Hot Temperature; D006801:Humans; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction; D016707:Tissue Fixation; D051197:Toll-Like Receptor 4 / Q000235:genetics; D014867:Water / Q000737:chemistry*; D014992:Xylenes / Q000737:chemistry*
C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4; D014992:Xylenes; D014867:Water; D005557:Formaldehyde; D004247:DNA
DNA extraction; FFPE tissue blocks; PCR