Probing Chromosome Dynamics in Bacillus subtilis.
Research over the last two decades has revealed that bacterial genomes are, in fact, highly organized. The goal of future research is to understand the molecular mechanisms underlying bacterial chromosome architecture and dynamics during the cell cycle. Here we discuss techniques that can be used with live cells to analyze chromosome structure and segregation in the gram-positive model organism Bacillus subtilis.
Centre for Bacterial Cell Biology, Institute for Cell & Molecular Biosciences, Newcastle University, Richardson Road, Newcastle Upon Tyne, NE2 4AX, UK.
Alan
A
Koh
Centre for Bacterial Cell Biology, Institute for Cell & Molecular Biosciences, Newcastle University, Richardson Road, Newcastle Upon Tyne, NE2 4AX, UK. heath.murray@newcastle.ac.uk.
Heath
H
Murray
D001412:Bacillus subtilis / Q000235:genetics*; D002455:Cell Division; D020090:Chromosome Segregation; D002876:Chromosomes, Bacterial / Q000737:chemistry / Q000502:physiology*; D004261:DNA Replication
Chromosome; DNA replication; DNA segregation; Fluorescent protein; Microscopy; Prokaryote