Design of polyelectrolyte core-shells with DNA to control TMPyP binding.
The interaction of DNA with 5,10,15,20-tetrakis(4-N-methylpyridiniumyl)porphyrin (TMPyP) in polyelectrolyte core-shells obtained via layer by layer adsorption of poly(sodium 4-styrenesulfonate), PSS, and poly(allylamine hydrochloride), PAH, polyelectrolytes was followed by steady state, time resolved fluorescence and by Fluorescence Lifetime Imaging Microscopy (FLIM). Our results show that DNA adsorption onto polyelectrolyte core-shell changes the TMPyP interaction within PSS/PAH core-shells structure and increase significantly the TMPyP uptake. Specific DNA/TMPyP interactions are also altered by DNA adsorption favouring porphyrin intercalation onto GC pair rich regions. Circular dichroism (CD) spectra reveal that DNA undergoes important conformational changes upon adsorption onto the core-shell surface, which are reverted upon TMPyP encapsulation.
Centro de Química Estrutural, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais 1, 1049-001 Lisboa, Portugal; Unidade de Química Orgânica e Produtos Naturais, Departamento de Química, Universidade de Aveiro, Portugal. Electronic address: vanda.serra@tecnico.ulisboa.pt.
Vanda Vaz
VV
Serra
Centro de Química Estrutural, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais 1, 1049-001 Lisboa, Portugal.
Raquel
R
Teixeira
Centro de Química Estrutural, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais 1, 1049-001 Lisboa, Portugal.
Suzana M
SM
Andrade
Centro de Química Estrutural, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais 1, 1049-001 Lisboa, Portugal. Electronic address: sbcosta@tecnico.ulisboa.pt.
Sílvia M B
SM
Costa
D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D015195:Drug Design; D017319:Photosensitizing Agents / Q000737:chemistry / Q000378:metabolism*; D000071228:Polyelectrolytes / Q000737:chemistry*; D011166:Porphyrins / Q000737:chemistry / Q000378:metabolism*
D017319:Photosensitizing Agents; D000071228:Polyelectrolytes; D011166:Porphyrins; C021096:tetra(4-N-methylpyridyl)porphine; D004247:DNA
DNA; Fluorescence Lifetime Imaging (FLIM); Polyelectrolyte core-shells; Porphyrins