Identification and determination of the inulin content in the roots of the Northeast Brazilian species Pombalia calceolaria L.
A polysaccharide was extracted from the roots of Pombalia calceolaria, a plant used in folk medicine in Northeastern Brazil, by decoction followed by precipitation with methanol, yielding a concentration of 13.0% w/w, and purification with acetone. The molar mass peak was estimated to be 4.0×10(3)Da using gel permeation chromatography (GPC). Polarized light photomicrography of histological sections revealed the presence of inulin in the cortical parenchyma. The chemical composition of inulin was identified by 1D and 2D NMR and FT-IR spectroscopy and the findings were compared with the literature. This is the first time inulin has been identified on FT-IR and NMR for the species Pombalia calceolaria.
Graduation Program of Pharmaceutical Science, Federal University of Ceará, Rua Capitão Francisco Pedro, 1210 Rodolfo Teófilo, 60430-160 Fortaleza, CE, Brazil. Electronic address: ana.georgina11@alu.ufc.br.
Ana Georgina Oliveira
AG
Pontes
Graduation Program of Pharmaceutical Science, Federal University of Ceará, Rua Capitão Francisco Pedro, 1210 Rodolfo Teófilo, 60430-160 Fortaleza, CE, Brazil.
Karine Lima
KL
Silva
Departament of Pharmacy, Federal University of Ceará, Rua Capitão Francisco Pedro, 1210 Rodolfo Teófilo, 60430-160 Fortaleza, CE, Brazil. Electronic address: said@ufc.br.
Said Gonçalvez da Cruz
SG
Fonseca
Departament of Biology, Federal University of Ceará, Av. Mister Hull, s/n-Antônio Bezerra, 60455-760 Fortaleza, CE, Brazil. Electronic address: arlete@ufc.br.
Arlete Aparecida
AA
Soares
Departament of Chemistry, Federal University of Ceará, Av. Mister Hull, s/n-Antônio Bezerra, 60455-760 Fortaleza, CE, Brazil. Electronic address: judith@dqoi.ufc.br.
Judith Pessoa de Andrade
JP
Feitosa
Visiting Researcher Emeritus - FAPERJ/UENF, Chemistry Lab - CCT-UENF/PPGQO-DEQUIMECE-UFRJ, Av. Alberto Lamego, 2000, 28013-602, Campos dos Goytacazes, RJ, Brazil. Electronic address: braz@uenf.br.
Raimundo
R
Braz-Filho
Departament of Pharmacy, Federal University of Ceará, Rua Capitão Francisco Pedro, 1210 Rodolfo Teófilo, 60430-160 Fortaleza, CE, Brazil.
Nirla Rodrigues
NR
Romero
Graduation Program of Pharmaceutical Science, Federal University of Ceará, Rua Capitão Francisco Pedro, 1210 Rodolfo Teófilo, 60430-160 Fortaleza, CE, Brazil; Departament of Pharmacy, Federal University of Ceará, Rua Capitão Francisco Pedro, 1210 Rodolfo Teófilo, 60430-160 Fortaleza, CE, Brazil. Electronic address: mambandeira@yahoo.com.br.
Mary Anne Medeiros
MA
Bandeira
D007444:Inulin / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D018517:Plant Roots / Q000737:chemistry*; D029649:Violaceae / Q000737:chemistry*
D007444:Inulin
Inulin; Pombalia calceolaria; Roots; Structure identification