Protective Effects of Extracellular and Intracellular Polysaccharides on Hepatotoxicity by Hericium erinaceus SG-02.
The protective effects of extracellular and intracellular polysaccharides from Hericium erinaceus SG-02 on the CCl4-induced hepatic injury of mice were investigated in this work. By the analysis of GC, the extracellular polysaccharides (EPS) were composed of arabinose, mannose, galactose, and glucose with a ratio of 1:7:14:52, and the composition of intracellular polysaccharides (IPS) was rhamnose, xylose, mannose, galactose, and glucose with a ratio of 3:4:7:14:137. The model of hepatic injury of mice was induced by CCl4 and three tested levels (200, 400, and 800 mg/kg) of EPS and IPS were set as the experimental group. Results showed that the aspartate aminotransferase and glutamic pyruvic transaminase activities in serum were reduced by the supplement of EPS and IPS, while the blood lipid levels including cholesterol, triglyceride, and albumin were improved. In liver tissue, the lipid peroxidation and malondialdehyde were largely decreased, and the superoxide dismutase and catalase activities were significantly increased. The evidence demonstrated that the EPS and IPS of H. erinaceus SG-02 were protective for liver injury. The histopathological observations of mice liver slices indicated that EPS and IPS had obvious effects on liver protection.
College of Life Science, Shandong Agricultural University, Tai'an, 271018, Shandong, People's Republic of China.
Fangyuan
F
Cui
Shandong Agricultural Technology Extending Station, Jinan, 250100, Shandong, People's Republic of China.
Xia
X
Gao
College of Life Science, Shandong Agricultural University, Tai'an, 271018, Shandong, People's Republic of China.
Jianjun
J
Zhang
College of Life Science, Shandong Agricultural University, Tai'an, 271018, Shandong, People's Republic of China.
Min
M
Liu
College of Life Science, Shandong Agricultural University, Tai'an, 271018, Shandong, People's Republic of China.
Chen
C
Zhang
College of Life Science, Shandong Agricultural University, Tai'an, 271018, Shandong, People's Republic of China.
Nuo
N
Xu
College of Life Science, Shandong Agricultural University, Tai'an, 271018, Shandong, People's Republic of China.
Huajie
H
Zhao
College of Life Science, Shandong Agricultural University, Tai'an, 271018, Shandong, People's Republic of China.
Lin
L
Lin
College of Life Science, Shandong Agricultural University, Tai'an, 271018, Shandong, People's Republic of China.|Quality and Safety Monitoring Center of Animal Products, Jinan, 250002, Shandog, People's Republic of China.
Meng
M
Zhou
College of Life Science, Shandong Agricultural University, Tai'an, 271018, Shandong, People's Republic of China. jiale0525@163.com.
Le
L
Jia
D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D001487:Basidiomycota / Q000737:chemistry*; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy / Q000378:metabolism / Q000517:prevention & control*; D002784:Cholesterol / Q000097:blood; D006801:Humans; D015227:Lipid Peroxidation; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D011134:Polysaccharides / Q000008:administration & dosage* / Q000737:chemistry; D020011:Protective Agents / Q000008:administration & dosage*
D011134:Polysaccharides; D020011:Protective Agents; D008315:Malondialdehyde; D002784:Cholesterol; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase