Fabrication of nanocomposite mat through incorporating bioactive glass particles into gelatin/poly(ε-caprolactone) nanofibers by using Box-Behnken design.
The current research was conducted to propose a nanocomposite material, which could be suitable to be used as a scaffold for bone tissue engineering applications. For this purpose, nanocomposite fibers of gelatin, poly(ε-caprolactone) (PCL), and bioactive glass were successfully fabricated via electrospinning process. In this context, response surface methodology based on a three-level, four-variable Box-Behnken design was adopted as an optimization tool to choose the most appropriate parameter settings to obtain the desired fiber diameter. The investigation, based on a second order polynomial model, focused on the analysis of the effect of both solution and processing parameters on the fiber diameter and its standard deviation. In optimum conditions (bioactive glass content of 7.5% (w/v), applied voltage of 25kV, tip-to-collector distance of 12.5cm, and flow rate of 1mL/h), the fiber diameter was found to be 584±337nm which was in good agreement with the predicted value by the developed models (523±290nm). Analytical tools such as scanning electron microscopy, X-ray diffraction analysis, Fourier transform infrared spectroscopy, and differential thermal analyzer were used for further evaluation of the optimized nanocomposite mat. The overall results showed that nanocomposite scaffolds could be promising candidates for tissue engineering applications.
Department of Chemical Engineering, Istanbul Technical University, Istanbul, Turkey. Electronic address: gonens@itu.edu.tr.
Seza Özge
SÖ
Gönen
Department of Chemical Engineering, Istanbul Technical University, Istanbul, Turkey.
Melek
M
Erol Taygun
Department of Chemical Engineering, Istanbul Technical University, Istanbul, Turkey.
Ayşen
A
Aktürk
Department of Chemical Engineering, Istanbul Technical University, Istanbul, Turkey.
Sadriye
S
Küçükbayrak
D002516:Ceramics / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D011091:Polyesters / Q000737:chemistry*; D023822:Tissue Engineering
C022107:Bioglass; D011091:Polyesters; C016240:polycaprolactone; D005780:Gelatin
Electrospinning; Gelatin; Nanocomposite; Poly(ε-caprolactone); Response surface methodology