Determination of Arbutin in Rat Plasma Using Liquid Chromatography-Tandem Mass Spectrometry: Application to a Pharmacokinetic Study After Oral Administration of the Extract of Vaccinium vitis-idaea.
A rapid and sensitive bioassay based on liquid chromatography-tandem mass spectrometry (LC-MS-MS) has been developed and validated to measure arbutin in rat plasma. Sample preparation of plasma after the addition of indapamide as internal standard (IS) involved solid-phase extraction (SPE) on C18 cartridges. Reversed-phase chromatography using acetonitrile and 0.5% formic acid solution (pH 2.56) was used for separation in a run time of 4.0 min. The analytes were detected in the negative ion mode using selective reaction monitoring (SRM) of the transitions at m/z 271.2 → 107.8 for arbutin and 364.3 → 189.0 for indapamide. The method has the following performance characteristics: a reliable response range of 7.5-5250.0 ng/mL with correlation coefficients (r) of >0.995. The lower limit of quantitation (LLOQ) was 7.5 ng/mL. The intra- and inter-day precision and accuracy of the quality control (QC) samples at low, medium and high concentration levels showed ≤8.79% relative standard deviation (RSD) and -1.15 to 1.49% relative error (RE). The method was successfully applied to a pharmacokinetic study to measure arbutin in rats after extracts of Vaccinium vitis-idaea was orally administered.
The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.
Xiaorong
X
Wang
The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.
Chunhong
C
Zhang
The First People's Hospital of Taizhou in Zhejiang Province, Taizhou 318020, China.
Nina
N
Zheng
The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.
Jihong
J
Ma
The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.
Ye Fan
YF
Zhu