Expression Pattern of Matrix Metalloproteinase 20 (MMP20) in Human Tumors.
OBJECTIVE
Matrix metalloproteinase 20 (MMP20) is a member of the family of matrix metalloproteinases. Under normal conditions the expression of MMP20 is restricted to ameloblasts and odontoblasts. In order to identify a possible expression of MMP20 under pathological conditions, we investigated three major human tumor entities, i.e. colon, breast and lung tumors, on the mRNA and protein level.


METHODS
Real-time RT-PCR and immunocytochemical analyses of established human tumor cell lines were employed for our study; immunohistochemical analysis was performed on both primary tumors and normal control tissues.


RESULTS
MMP20 was identified on both the mRNA and the protein level in breast MCF-7, colon HT-29, and lung A549 cell lines. MMP20 was also detected in primary tumor tissue by immunohistochemistry.


CONCLUSIONS
MMP20 is a new potential candidate for tumor diagnosis or therapy.
Department of Prosthodontics, Preclinical Education and Material Science, University of Bonn, Bonn, Germany.
Dominik
D
Kraus
Department of Prosthodontics, Preclinical Education and Material Science, University of Bonn, Bonn, Germany.
Jan
J
Reckenbeil
Pathology of the University Hospital of Luebeck and the Leibuiz Research Center Borstel, Luebeck and Borstel, Germany.
Sven
S
Perner
Oral Cell Biology Group, Department of Periodontology, Operative and Preventive Dentistry, University of Bonn, Bonn, Germany.
Jochen
J
Winter
Neuro- and Tumor Cell Biology Group, Department of Nuclear Medicine, University Hospital of Bonn, Bonn, Germany r.probstmeier@uni-bonn.de.
Rainer
R
Probstmeier
D006801:Humans; D007150:Immunohistochemistry; D061986:MCF-7 Cells; D053519:Matrix Metalloproteinase 20 / Q000032:analysis* / Q000235:genetics / Q000502:physiology; D009369:Neoplasms / Q000201:enzymology*
C507111:MMP20 protein, human; D053519:Matrix Metalloproteinase 20
Ameloblast; MMP20; enamelysin; metalloproteinase; odontoblast