A sensitive acetylcholinesterase biosensor based on gold nanorods modified electrode for detection of organophosphate pesticide.
A sensitive amperometric acetylcholinesterase (AChE) biosensor, based on gold nanorods (AuNRs), was developed for the detection of organophosphate pesticide. Compared with Au@Ag heterogeneous NRs, AuNRs exhibited excellent electrocatalytic properties, which can electrocatalytically oxidize thiocholine, the hydrolysate of acetylthiocholine chloride (ATCl) by AChE at +0.55V (vs. SCE). The AChE/AuNRs/GCE biosensor was fabricated on basis of the inhibition of AChE activity by organophosphate pesticide. The biosensor could detect paraoxon in the linear range from 1nM to 5μM and dimethoate in the linear range from 5nM to 1μM, respectively. The detection limits of paraoxon and dimethoate were 0.7nM and 3.9nM, which were lower than the reported AChE biosensor. The proposed biosensor could restore to over 95% of its original current, which demonstrated the good reactivation. Moreover, the biosensor can be applicable to real water sample measurement. Thus, the biosensor exhibited low applied potential, high sensitivity and good stability, providing a promising tool for analysis of pesticides.
Institute for Biosensing, and College of Chemistry and Chemical Engineering, Qingdao University, Qingdao 266071, China; Laboratory for Biosensing, Qingdao Institute of Bioenergy & Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Road, Qingdao 266101, China.
Qiaolin
Q
Lang
Institute for Biosensing, and College of Chemistry and Chemical Engineering, Qingdao University, Qingdao 266071, China; Laboratory for Biosensing, Qingdao Institute of Bioenergy & Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Road, Qingdao 266101, China.
Lei
L
Han
Institute for Biosensing, and College of Chemistry and Chemical Engineering, Qingdao University, Qingdao 266071, China.
Chuantao
C
Hou
Institute for Biosensing, and College of Chemistry and Chemical Engineering, Qingdao University, Qingdao 266071, China.
Fei
F
Wang
Institute for Biosensing, and College of Chemistry and Chemical Engineering, Qingdao University, Qingdao 266071, China. Electronic address: liuah@qdu.edu.cn.
Aihua
A
Liu
D000110:Acetylcholinesterase / Q000378:metabolism*; D015374:Biosensing Techniques / Q000379:methods*; D002800:Cholinesterase Inhibitors / Q000032:analysis / Q000378:metabolism; D004117:Dimethoate / Q000032:analysis / Q000378:metabolism; D004800:Enzymes, Immobilized / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D006801:Humans; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D009943:Organophosphorus Compounds / Q000032:analysis* / Q000378:metabolism; D010261:Paraoxon / Q000032:analysis / Q000378:metabolism; D010575:Pesticides / Q000032:analysis* / Q000378:metabolism; D014867:Water / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000378:metabolism
D002800:Cholinesterase Inhibitors; D004800:Enzymes, Immobilized; D009943:Organophosphorus Compounds; D010575:Pesticides; D014874:Water Pollutants, Chemical; D014867:Water; D006046:Gold; D000110:Acetylcholinesterase; D010261:Paraoxon; D004117:Dimethoate
Acetylcholinesterase biosensor; Au@Ag heterogeneous NRs; Gold nanorods; Organophosphate pesticide