Metagenomic insights into ultraviolet disinfection effects on antibiotic resistome in biologically treated wastewater.
High-throughput sequencing-based metagenomic approaches were used to comprehensively investigate ultraviolet effects on the microbial community structure, and diversity and abundance of antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) in biologically treated wastewater. After ultraviolet radiation, some dominant genera, like Aeromonas and Halomonas, in the wastewater almost disappeared, while the relative abundance of some minor genera including Pseudomonas and Bacillus increased dozens of times. Metagenomic analysis showed that 159 ARGs within 14 types were detectable in the samples, and the radiation at 500 mJ/cm(2) obviously increased their total relative abundance from 31.68 ppm to 190.78 ppm, which was supported by quantitative real time PCR. As the dominant persistent ARGs, multidrug resistance genes carried by Pseudomonas and bacitracin resistance gene bacA carried by Bacillus mainly contributed to the ARGs abundance increase. Bacterial community shift and MGEs replication induced by the radiation might drive the resistome alteration. The findings may shed new light on the mechanism behind the ultraviolet radiation effects on antibiotic resistance in wastewater.
State Key Laboratory of Pollution Control and Resource Reuse, Environmental Health Research Center, School of the Environment, Nanjing University, Nanjing 210023, China.
Qing
Q
Hu
State Key Laboratory of Pollution Control and Resource Reuse, Environmental Health Research Center, School of the Environment, Nanjing University, Nanjing 210023, China. Electronic address: zhangxx@nju.edu.cn.
Xu-Xiang
XX
Zhang
State Key Laboratory of Pollution Control and Resource Reuse, Environmental Health Research Center, School of the Environment, Nanjing University, Nanjing 210023, China.
Shuyu
S
Jia
State Key Laboratory of Pollution Control and Resource Reuse, Environmental Health Research Center, School of the Environment, Nanjing University, Nanjing 210023, China.
Kailong
K
Huang
State Key Laboratory of Pollution Control and Resource Reuse, Environmental Health Research Center, School of the Environment, Nanjing University, Nanjing 210023, China.
Junying
J
Tang
State Key Laboratory of Pollution Control and Resource Reuse, Environmental Health Research Center, School of the Environment, Nanjing University, Nanjing 210023, China.
Peng
P
Shi
State Key Laboratory of Pollution Control and Resource Reuse, Environmental Health Research Center, School of the Environment, Nanjing University, Nanjing 210023, China. Electronic address: linye@nju.edu.cn.
Lin
L
Ye
State Key Laboratory of Pollution Control and Resource Reuse, Environmental Health Research Center, School of the Environment, Nanjing University, Nanjing 210023, China.
Hongqiang
H
Ren
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004203:Disinfection; D004352:Drug Resistance, Microbial / Q000235:genetics; D056186:Metagenomics; D014466:Ultraviolet Rays; D062065:Wastewater / Q000382:microbiology*
D000900:Anti-Bacterial Agents; D062065:Wastewater
Antibiotic resistance; High-throughput sequencing; Metagenomics; Microbial community; Mobile genetic elements; Ultraviolet disinfection