Preparation and sustainable release of modified konjac glucomannan/chitosan nanospheres.
Biodegradable and biocompatible polymer nanospheres are useful materials for controlled drug delivery. In the present study, novel composite nanospheres were prepared from carboxymethyl konjac glucomannan (CKGM) and 2-hydroxypropyl trimethyl ammonium chloride chitosan (HACC) as a vaccine delivery vehicle by electrostatic complexation in a neutral aqueous solution without the use of chemical crosslinkers. By altering the CKGM and HACC concentrations, the average CKGM/HACC particle size could be tuned from approximately 600nm to 1460nm and the zeta potential from 39mV to 50mV. Furthermore, using ovalbumin (OVA) as a model molecule for vaccines, various parameters were determined to affect the CKGM/HACC nanosphere encapsulation efficiency and in vitro controlled release properties. Under optimum conditions, the OVA encapsulation efficiency of CKGM/HACC nanospheres was 71.8%, while sustained and continuous in vitro OVA release over a period of more than 24h was observed. Therefore, CKGM/HACC nanospheres are novel drug delivery carriers with great potential for medical applications.
State Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan 430070, PR China.
Congjiao
C
Shi
State Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan 430070, PR China.
Pei
P
Zhu
State Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan 430070, PR China.
Na
N
Chen
State Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan 430070, PR China. Electronic address: xzye@mail.hzau.edu.cn.
Xiaozhou
X
Ye
State Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan 430070, PR China. Electronic address: yunwang123@126.com.
Yun
Y
Wang
State Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan 430070, PR China.
Shaobo
S
Xiao
D000818:Animals; D002412:Cations; D002645:Chickens; D048271:Chitosan / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D003692:Delayed-Action Preparations; D065546:Drug Liberation; D008351:Mannans / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D054118:Nanospheres / Q000737:chemistry* / Q000648:ultrastructure; D010047:Ovalbumin / Q000378:metabolism; D010316:Particle Size; D017550:Spectroscopy, Fourier Transform Infrared; D055672:Static Electricity
D002412:Cations; D003692:Delayed-Action Preparations; D008351:Mannans; C118071:N-trimethyl chitosan chloride; C538690:carboxymethyl konjac glucomannan; D010047:Ovalbumin; D048271:Chitosan
Chitosan; Konjac glucomannan; Nanospheres; Ovalbumin; Sustained release