Differentiation of functional endothelial cells from human induced pluripotent stem cells: A novel, highly efficient and cost effective method.
Endothelial cells derived from human induced pluripotent stem cells (hiPSC- EC) are of significant value for research on human vascular development, in vitro disease models and drug screening. Here we report an alternative, highly efficient and cost-effective simple three step method (mesoderm induction, endothelial cell differentiation and endothelial cell expansion) to differentiate hiPSC directly into endothelial cells. We demonstrate that efficiency of described method to derive CD31+ and VE-Cadherin+ double positive cells is higher than 80% in 12 days. Most notably we established that hiPSC-EC differentiation efficacy depends on optimization of both mesoderm differentiation and endothelial cell differentiation steps.
Department of Cardiology and Pneumology, University Medical Center of Göttingen, Georg August University, 37075 Göttingen, Germany.
Xiaopeng
X
Liu
Department of Cardiology and Pneumology, University Medical Center of Göttingen, Georg August University, 37075 Göttingen, Germany.
Jing
J
Qi
Department of Cardiology and Pneumology, University Medical Center of Göttingen, Georg August University, 37075 Göttingen, Germany; DZHK (German Centre for Cardiovascular Research) Partner Site Göttingen, 37075 Göttingen, Germany.
Xingbo
X
Xu
Department of Nephrology and Rheumatology, University Medical Center of Göttingen, Georg August University, 37075 Göttingen, Germany; DZHK (German Centre for Cardiovascular Research) Partner Site Göttingen, 37075 Göttingen, Germany.
Michael
M
Zeisberg
Department of Cardiology and Pneumology, University Medical Center of Göttingen, Georg August University, 37075 Göttingen, Germany; Department of Pharmacology and Toxicology, TU Dresden, 01307 Dresden, Germany.
Kaomei
K
Guan
Department of Cardiology and Pneumology, University Medical Center of Göttingen, Georg August University, 37075 Göttingen, Germany; DZHK (German Centre for Cardiovascular Research) Partner Site Göttingen, 37075 Göttingen, Germany. Electronic address: elisabeth.zeisberg@med.uni-goettingen.de.
Elisabeth M
EM
Zeisberg
D015703:Antigens, CD / Q000378:metabolism; D015820:Cadherins / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000235:genetics*; D019070:Cell Lineage / Q000235:genetics; D049109:Cell Proliferation; D042783:Endothelial Cells / Q000166:cytology*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D039904:Pluripotent Stem Cells; D000099152:Cadherin 5
D015703:Antigens, CD; D015820:Cadherins; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D000099152:Cadherin 5
EC; Highly efficient differentiation method; hiPSC