Structural and functional studies of a large winged Z-DNA-binding domain of Danio rerio protein kinase PKZ.
The Z-DNA-binding domain of PKZ from zebrafish (Danio rerio; drZαPKZ ) contains the largest β-wing among known Z-DNA-binding domains. To elucidate the functional implication of the β-wing, we solved the crystal structure of apo-drZαPKZ . Structural comparison with its Z-DNA-bound form revealed a large conformational change within the β-wing during Z-DNA binding. Biochemical studies of protein mutants revealed that two basic residues in the β-wing are responsible for Z-DNA recognition as well as fast B-Z transition. Therefore, the extra basic residues in the β-wing of drZαPKZ are necessary for the fast B-Z transition activity.
Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon, Korea.
Vinod Kumar
VK
Subramani
Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon, Korea.
Doyoun
D
Kim
Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon, Korea.
Kyunghee
K
Yun
Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon, Korea.
Kyeong Kyu
KK
Kim
D000818:Animals; D018360:Crystallography, X-Ray; D043542:DNA, Z-Form / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D011494:Protein Kinases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D017433:Protein Structure, Secondary; D013329:Structure-Activity Relationship; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D043542:DNA, Z-Form; D029961:Zebrafish Proteins; C583881:PKZ protein, zebrafish; D011494:Protein Kinases
B-Z transition; PKZ; Z-DNA; Zα; zebrafish