Efficient production of acetoin in Saccharomyces cerevisiae by disruption of 2,3-butanediol dehydrogenase and expression of NADH oxidase.
Acetoin is widely used in food and cosmetic industry as taste and fragrance enhancer. For acetoin production in this study, Saccharomyces cerevisiae JHY605 was used as a host strain, where the production of ethanol and glycerol was largely eliminated by deleting five alcohol dehydrogenase genes (ADH1, ADH2, ADH3, ADH4, and ADH5) and two glycerol 3-phosphate dehydrogenase genes (GPD1 and GPD2). To improve acetoin production, acetoin biosynthetic genes from Bacillus subtilis encoding α-acetolactate synthase (AlsS) and α-acetolactate decarboxylase (AlsD) were overexpressed, and BDH1 encoding butanediol dehydrogenase, which converts acetoin to 2,3-butanediol, was deleted. Furthermore, by NAD(+) regeneration through overexpression of water-forming NADH oxidase (NoxE) from Lactococcus lactis, the cofactor imbalance generated during the acetoin production from glucose was successfully relieved. As a result, in fed-batch fermentation, the engineered strain JHY617-SDN produced 100.1 g/L acetoin with a yield of 0.44 g/g glucose.
School of Chemical and Biological Engineering, Seoul National University, Institute of Chemical and Processes, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea.
Sang-Jeong
SJ
Bae
School of Chemical and Biological Engineering, Seoul National University, Institute of Chemical and Processes, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea.
Sujin
S
Kim
School of Chemical and Biological Engineering, Seoul National University, Institute of Chemical and Processes, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea.
Ji-Sook
JS
Hahn
D000093:Acetoin / Q000378:metabolism*; D000095:Acetolactate Synthase / Q000235:genetics / Q000378:metabolism; D000429:Alcohol Oxidoreductases / Q000235:genetics*; D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002262:Carboxy-Lyases / Q000235:genetics / Q000378:metabolism; D005285:Fermentation; D017353:Gene Deletion; D007218:Industrial Microbiology / Q000379:methods; D017045:Lactococcus / Q000201:enzymology / Q000235:genetics; D009097:Multienzyme Complexes / Q000235:genetics* / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D009097:Multienzyme Complexes; D000093:Acetoin; D000429:Alcohol Oxidoreductases; C019801:butanediol dehydrogenase; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases; D000095:Acetolactate Synthase; D002262:Carboxy-Lyases; C023746:acetolactate decarboxylase