Bronchodilatory, antitussive and anti-inflammatory effect of morin in the setting of experimentally induced allergic asthma.
OBJECTIVE
Using an experimental model of allergic asthma, we evaluated the anti-asthmatic potential of polyphenol flavonol derivate morin after either acute or long-term treatment of male OVA-sensitised guinea pigs.


METHODS
The following methods were used in experiments: the in-vitro tracheal smooth muscle contraction induced by histamine; the changes in specific airway resistance (sRaw) to histamine and the sensitivity of a chemically induced cough reflex both via an in-vivo method; the serum and BALF concentrations' analysis of the inflammatory cytokines interleukin IL-4, IL-5, IL-13; and lung tissue infiltration by eosinophils and mastocytes.


RESULTS
Our data show that acute morin (30 mg/kg) and chronic 21-day morin (30 mg/kg/day) administration had a comparable antitussive efficiency with opioid antitussive codeine. Acute morin bronchodilatory activity defined by in-vivo sRaw decline did not reach SABA salbutamol effect. However, bronchodilatory efficiency of morin after long-term administration was by 34% higher as effect of LABA salmeterol. The 21-day morin treatment of OVA-sensitised guinea pigs reduced the serum, BALF levels of IL-4 and IL-13, lung tissue eosinophil and mastocyte infiltration comparable with corticosteroid budesonide.


CONCLUSIONS
In summary, morin represents very rational target for additional studies as potential substance for control as well as prevention of asthma inflammation and symptoms.
Department of Pharmacology, BioMed, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Martin, Slovakia.
Sona
S
Franova
Department of Pharmacology, BioMed, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Martin, Slovakia.
Ivana
I
Kazimierova
Department of Pharmacology, BioMed, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Martin, Slovakia.
Lenka
L
Pappova
Department of Pharmacology, BioMed, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Martin, Slovakia.
Marta
M
Joskova
Department of Pathological Anatomy, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Martin, Slovakia.
Lukas
L
Plank
Department of Pharmacology, BioMed, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Martin, Slovakia.
Martina
M
Sutovska
D000403:Airway Resistance; D000818:Animals; D018927:Anti-Asthmatic Agents / Q000494:pharmacology / Q000627:therapeutic use; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000627:therapeutic use; D000996:Antitussive Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001249:Asthma / Q000188:drug therapy / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid; D001993:Bronchodilator Agents / Q000494:pharmacology* / Q000627:therapeutic use; D003371:Cough / Q000139:chemically induced / Q000188:drug therapy; D016207:Cytokines / Q000378:metabolism; D004804:Eosinophils; D005419:Flavonoids / Q000494:pharmacology* / Q000627:therapeutic use; D006168:Guinea Pigs; D006632:Histamine; D006967:Hypersensitivity; D007249:Inflammation / Q000188:drug therapy / Q000378:metabolism; D008168:Lung / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D010047:Ovalbumin; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D014132:Trachea; D047309:Flavones
D018927:Anti-Asthmatic Agents; D000893:Anti-Inflammatory Agents; D000996:Antitussive Agents; D001993:Bronchodilator Agents; D016207:Cytokines; D005419:Flavonoids; D006632:Histamine; D010047:Ovalbumin; D010936:Plant Extracts; C008548:morin; D047309:Flavones
airway reactivity; allergic asthma; cough; cytokines; polyphenol morin