Whole-Cell Patch-Clamp Recording of Mouse and Rat Inner Hair Cells in the Intact Organ of Corti.
Whole-cell patch clamping is a widely applied method to record currents across the entire membrane of a cell. This protocol describes application of this method to record currents from the sensory inner hair cells in the intact auditory sensory epithelium, the organ of Corti, isolated from rats or mice. This protocol particularly outlines the basic equipment required, provides instructions for the preparation of solutions and small equipment items, and methodology for recording voltage-activated and evoked synaptic currents from the inner hair cells.
Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (CONICET), Vuelta de Obligado 2490, C. A., Buenos Aires, 1428, Argentina.
Juan D
JD
Goutman
Department of Otorhinolaryngology, University Medical Center Groningen, Hanzeplein 1, Groningen, GZ, 9713, The Netherlands. s.pyott@umcg.nl.
Sonja J
SJ
Pyott
D000200:Action Potentials; D000818:Animals; D005071:Evoked Potentials; D006199:Hair Cells, Auditory, Inner / Q000166:cytology / Q000502:physiology*; D051379:Mice; D009925:Organ of Corti / Q000166:cytology / Q000502:physiology*; D018408:Patch-Clamp Techniques / Q000295:instrumentation* / Q000379:methods; D051381:Rats
Cochlea; Evoked synaptic current; Inner hair cell; Organ of Corti; Voltage clamp; Voltage-activated current; Whole-cell patch-clamp