Optical Coherence Tomography to Measure Sound-Induced Motions Within the Mouse Organ of Corti In Vivo.
The measurement of mechanical vibrations within the living cochlea is critical to understanding the first nonlinear steps in auditory processing, hair cell stimulation, and cochlear amplification. However, it has proven to be a challenging endeavor. This chapter describes how optical coherence tomography (OCT) can be used to measure vibrations within the tissues of the organ of Corti. These experimental measurements can be performed within the unopened cochlea of living mice routinely and reliably.
Department of Otolaryngology-Head and Neck Surgery, Stanford University, 300 Pasteur Dr., Edwards R113, Stanford, CA, 94305, USA.
Zina
Z
Jawadi
Department of Biomedical Engineering, Texas A&M University, 5045 Emerging Technology Building, College Station, TX, 77843, USA.
Brian E
BE
Applegate
Department of Otolaryngology-Head and Neck Surgery, Stanford University, 300 Pasteur Dr., Edwards R113, Stanford, CA, 94305, USA. joghalai@stanford.edu.
John S
JS
Oghalai
D000161:Acoustic Stimulation; D000818:Animals; D001489:Basilar Membrane / Q000502:physiology; D001696:Biomechanical Phenomena; D051379:Mice; D009925:Organ of Corti / Q000502:physiology*; D013016:Sound; D013680:Tectorial Membrane / Q000502:physiology; D041623:Tomography, Optical Coherence / Q000295:instrumentation* / Q000379:methods; D014732:Vibration
Basilar membrane; Biomechanics; Cochlear mechanics; In vivo imaging; Sound encoding; Tectorial membrane; Vibrometry