Fatty liver disease induced by perfluorooctane sulfonate: Novel insight from transcriptome analysis.
Perfluorooctane sulfonate (PFOS), a hepato-toxicant and potential non-genotoxic carcinogen, was widely used in industrial and commercial products. Recent studies have revealed the ubiquitous occurrence of PFOS in the environment and in humans worldwide. The widespread contamination of PFOS in human serum raised concerns about its long-term toxic effects and its potential risks to human health. Using fatty liver mutant foie gras (fgr(-/-))/transport protein particle complex 11 (trappc11(-/-)) and PFOS-exposed wild-type zebrafish embryos as the study model, together with RNA sequencing and comparative transcriptomic analysis, we identified 499 and 1414 differential expressed genes (DEGs) in PFOS-exposed wild-type and trappc11 mutant zebrafish, respectively. Also, the gene ontology analysis on common deregulated genes was found to be associated with different metabolic processes such as the carbohydrate metabolic process, glycerol ether metabolic process, mannose biosynthetic process, de novo' (Guanosine diphosphate) GDP-l-fucose biosynthetic process, GDP-mannose metabolic process and galactose metabolic process. Ingenuity Pathway Analysis further highlighted that these deregulated gene clusters are closely related to hepatitis, inflammation, fibrosis and cirrhosis of liver cells, suggesting that PFOS can cause liver pathogenesis and non-alcoholic fatty liver disease in zebrafish. The transcriptomic alterations revealed may serve as biomarkers for the hepatotoxic effect of PFOS.
Department of Biology, Hong Kong Baptist University, Hong Kong SAR, China; Faculty of Agriculture, Kyushu University, Fukuoka, Japan. Electronic address: kftse@agr.kyushu-u.ac.jp.
William Ka
WK
Fai Tse
School of Life Sciences, State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong SAR, China. Electronic address: marcowanger@gmail.com.
Jing Woei
JW
Li
School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong SAR, China; The State Key Laboratory in Marine Pollution, Hong Kong SAR, China. Electronic address: annatse@hku.hk.
Anna Chung
AC
Kwan Tse
School of Life Sciences, State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong SAR, China. Electronic address: tf.chan@cuhk.edu.hk.
Ting Fung
TF
Chan
Department of Biology, Hong Kong Baptist University, Hong Kong SAR, China. Electronic address: moonjeff14@gmail.com.
Jeff Cheuk
JC
Hin Ho
The State Key Laboratory in Marine Pollution, Hong Kong SAR, China; Department of Science and Environmental Studies, Institute of Education, Hong Kong SAR, China. Electronic address: rudolfwu@hkied.edu.hk.
Rudolf Shiu
RS
Sun Wu
Department of Biology, Hong Kong Baptist University, Hong Kong SAR, China; The State Key Laboratory in Marine Pollution, Hong Kong SAR, China. Electronic address: ckcwong@hkbu.edu.hk.
Chris Kong
CK
Chu Wong
Department of Biology and Chemistry, City University of Hong Kong, Hong Kong SAR, China. Electronic address: kengplai@cityu.edu.hk.
Keng Po
KP
Lai
D017738:Alkanesulfonic Acids / Q000633:toxicity*; D000818:Animals; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D005234:Fatty Liver / Q000139:chemically induced / Q000235:genetics / Q000473:pathology*; D005466:Fluorocarbons / Q000633:toxicity*; D020869:Gene Expression Profiling; D006505:Hepatitis / Q000209:etiology / Q000473:pathology*; D022781:Hepatocytes; D006801:Humans; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000235:genetics / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015027:Zebrafish / Q000378:metabolism
D017738:Alkanesulfonic Acids; D005466:Fluorocarbons; D012333:RNA, Messenger; C076994:perfluorooctane sulfonic acid
Environmental pollutant; Hepatotoxicity; Perfluorooctane sulfonate; Transcriptome; Zebrafish