Dosage effects of Waxy gene on the structures and properties of corn starch.
The objective of this study was to understand dosage effects of the Waxy gene on the structures of amylose and amylopectin and on the properties of corn starch. Reciprocal crossing of isogenic normal and waxy corn lines was conducted to develop hybrids with different dosages (0, 1, 2, 3) of Waxy gene in the endosperm. The amylose content of starch and proportions of branch chains of DP 17-30 and extra-long branch chains (DP>100) of amylopectin were positively correlated with the Waxy-gene dosage. Proportions of short (DP<17) and long branch-chains (DP 30-80), however, were negatively correlated with the Waxy-gene dosage. The gelatinization conclusion-temperature and temperature-range of the starch were negatively correlated with the Waxy-gene dosage, indicating that amylose facilitated dissociation of the surrounding crystalline regions. These results helped us understand the function of granule-bound starch synthase I in the biosynthesis of amylose and amylopectin and impacts of Waxy-gene dosages on the properties of corn starch.
Department of Food Science and Human Nutrition, Iowa State University, Ames, IA 50011, USA.
Hanyu
H
Yangcheng
USDA-ARS Plant Introduction Research Unit, and Department of Agronomy, Iowa State University, Ames, IA 50011, USA.
Michael
M
Blanco
USDA-ARS Plant Introduction Research Unit, and Department of Agronomy, Iowa State University, Ames, IA 50011, USA.
Candice
C
Gardner
Department of Food Science and Human Nutrition, Iowa State University, Ames, IA 50011, USA; Department of Food Science and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou, Henan, China.
Xuehong
X
Li
Department of Food Science and Human Nutrition, Iowa State University, Ames, IA 50011, USA. Electronic address: jjane@iastate.edu.
Jay-Lin
JL
Jane
D000687:Amylopectin / Q000737:chemistry; D000688:Amylose / Q000737:chemistry; D018628:Gene Dosage; D008055:Lipids / Q000032:analysis; D008970:Molecular Weight; D010940:Plant Proteins / Q000235:genetics*; D013213:Starch / Q000737:chemistry*; D013214:Starch Synthase / Q000235:genetics*; D013696:Temperature; D003313:Zea mays / Q000737:chemistry* / Q000235:genetics*
D008055:Lipids; D010940:Plant Proteins; C060756:waxy protein, plant; D013213:Starch; D000688:Amylose; D000687:Amylopectin; D013214:Starch Synthase
Branch-chain length distribution; Dosage effects; Starch properties; Waxy gene