Nanogels fabricated from bovine serum albumin and chitosan via self-assembly for delivery of anticancer drug.
In this study, bovine serum albumin (BSA) and chitosan (CS) were used to prepare BSA-CS nanogels by a simple green self-assembly technique. Then the nanogels were successfully used to entrap doxorubicin hydrochloride (DOX) with an entrapment ratio of 46.3%, aiming to realize the slow-release effect and lower the cytotoxicity of DOX. The IC50 values of DOX-loaded BSA-CS (DOX-BSA-CS) and free DOX obtained by MTT assay in SGC7901 cells were 0.22 and 0.05μg/mL, respectively. The cytotoxicity of DOX significantly decreased within 24h after encapsulation by the nanogels, indicating that the loaded drug could slowly release within 24h and the BSA-CS was a good slow release system. The cellular uptake experiments indicated DOX-BSA-CS diffused faster into the cancer cell than the bare drug. The flow cytometry and TUNEL assay proved DOX-BSA-CS could induce a larger apoptosis proportion of gastric cancer cells 7901 than the bare drug and it is promising to be used for curing gastric cancer.
College of Food Science and Technology, Huazhong Agricultural University, Hubei, Wuhan 430070, China; Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of Education, China.
Yuntao
Y
Wang
Science and Technology College of Hubei University for Nationalities, China.
Shasha
S
Xu
College of Food Science and Technology, Huazhong Agricultural University, Hubei, Wuhan 430070, China; Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of Education, China.
Wenfei
W
Xiong
College of Food Science and Technology, Huazhong Agricultural University, Hubei, Wuhan 430070, China; Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of Education, China.
Yaqiong
Y
Pei
College of Food Science and Technology, Huazhong Agricultural University, Hubei, Wuhan 430070, China; Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of Education, China.
Bin
B
Li
College of Food Science and Technology, Huazhong Agricultural University, Hubei, Wuhan 430070, China; Key Laboratory of Environment Correlative Dietology (Huazhong Agricultural University), Ministry of Education, China. Electronic address: yijiechen@mail.hzau.edu.cn.
Yijie
Y
Chen
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D004058:Diffusion; D004317:Doxorubicin / Q000494:pharmacology*; D004337:Drug Carriers / Q000737:chemistry*; D006801:Humans; D020287:In Situ Nick-End Labeling; D000080385:Nanogels; D011092:Polyethylene Glycols / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000473:pathology; D014407:Tumor Cells, Cultured
D000903:Antibiotics, Antineoplastic; D004337:Drug Carriers; D000080385:Nanogels; C413692:polyethylene glycol polyethyleneimine nanogel; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D004317:Doxorubicin; D011094:Polyethyleneimine; D048271:Chitosan
Anticancer; Bovine serum albumin; Chitosan; Doxorubicin hydrochloride; Nanogels; Self-assembly