Photocatalytic degradation of nitrophenol using biologically active Phyllanthus emblica seed extract.
The present study deals about the vicinity of phytochemicals present in the Phyllanthus emblica (P. emblica) seed extract. The bio-active compounds present in the methanolic seed extract have been identified using Gas Chromatography Mass Spectroscopy (GC-MS)·The antioxidant activity of P. emblica seed extract was evaluated using assistance of DPPH (2,2-Diphenyl-1-picrylhydrazyl) assay. The determination of total phenol and flavonoid substance were contemplated. Further blood clot lysis activity was also done to check the percentage of clot lysis in methanolic seed extract. The result proved that seed extract has potential application. The GCMS results of P. emblica suggest that Octyl-β-d-Glucopyranoside is present in major quantity. The work has been designed towards the degradation of 2-nitrophenol and 4-nitrophenol using P. emblica methanolic seed extract. The progress of nitrophenol degradation has been observed in UV-visible spectroscopy. At 5min duration, the 4-nitrophenol has been degraded up to 82.42%. This may be due to the presence of secondary metabolites such as alkaloids, carbohydrate and phenols in the P. emblica seed extract. The seed extract showed good scavenging activity which resulted in IC50 value of 85.92μg/mL. The total phenol and flavonoid content present in the extract were 48.242 and 12.72mg/mL. Also the seed extract showed good lysis when compared to the standard streptokinase.
Plant Biotechnology Division, School of Biosciences and Technology, VIT University, Vellore - 632 014, Tamilnadu, India.
Murugesan
M
Dinesh
Chemistry of Heterocycles & Natural Product Research Laboratory, Department of Chemistry, School of Advanced Sciences, VIT University, Vellore 632 014, Tamilnadu, India. Electronic address: mohanaroopan.s@gmail.com.
Selvaraj Mohana
SM
Roopan
Plant Biotechnology Division, School of Biosciences and Technology, VIT University, Vellore - 632 014, Tamilnadu, India.
Chinnadurai Immanuel
CI
Selvaraj
D002384:Catalysis; D016166:Free Radical Scavengers / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D005960:Glucosides / Q000032:analysis / Q000302:isolation & purification; D008027:Light; D009596:Nitrophenols / Q000737:chemistry*; D010782:Photolysis / Q000528:radiation effects; D029842:Phyllanthus emblica / Q000737:chemistry* / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet
D016166:Free Radical Scavengers; D005960:Glucosides; D009596:Nitrophenols; D010936:Plant Extracts; C018619:octyl-beta-D-glucoside; C045573:2-nitrophenol; C024836:4-nitrophenol
Antioxidant; Nitrophenol; Photocatalytic activity; Phyllanthus emblica seed; Phytochemical screening