Effects of platelet rich plasma on fascial healing in rats with fecal peritonitis
To evaluate the effects of platelet rich plasma (PRP) on the healing of fascia wherein peritonitis has been created.



Twenty eight Wistar Albino rats were divided into four groups. Only a primary fascial repair following laparotomy was performed on Group 1, a primary fascial repair performed and PRP treatment applied following laparotomy on Group 2, and a fecal peritonitis created following laparotomy and a primary fascial repair carried out on Group 3. A fecal peritonitis was created following laparotomy and primary fascial repair and PRP treatment on the fascia was carried out on Group 4.



TNF-α was found to be significantly lower in the control group (Group 1). It was detected at the highest level in the group in which fecal peritonitis was created and PRP applied (Group 4). TGF-β was determined as being significantly higher only in Group 4. Histopathologically, the differences between the groups in terms of cell infiltration and collagen deposition were not found to be significant.



When platelet rich plasma was given histologically and biochemicaly as wound healing parameters cellular infiltration, collagen accumulation, and tissue hydroxyiproline levels were not increased but neovascularization, fibroblast activation and TNF Alfa levels were increased and PRP accelerated wound healing.
Associate Professor, Department of General Surgery, Faculty of Medicine, Firat University, Elazig, Turkey. Design of the study, surgical procedures, statistical analysis., Firat University, Department of General Surgery, Faculty of Medicine, Firat University, Turkey.
Mustafa
M
Girgin
MD, Department of General Surgery, Faculty of Medicine, Firat University, Elazig, Turkey. Design of the study, surgical procedures, statistical analysis., Firat University, Department of General Surgery, Faculty of Medicine, Firat University, Turkey.
Kenan
K
Binnetoglu
MD, Department of General Surgery, Elazig Military Hospital, Elazig, Turkey. Interpretation of data, manuscript preparation and writing., , Department of General Surgery, Elazig Military Hospital, Elazig , Turkey.
Kazim
K
Duman
MD, Department of General Surgery, Elazig Training and Research Hospital, Elazig, Turkey. Statistical analysis, interpretation of data, manuscript preparation., Department of General Surgery, Elazig Training and Research Hospital, Elazig , Turkey.
Burhan Hakan
BH
Kanat
Full Professor, Department of General Surgery, Faculty of Medicine, Firat University, Elazig, Turkey. Design of the study, statistical analysis., Firat University, Department of General Surgery, Faculty of Medicine, Firat University, Elazig , Turkey.
Ziya
Z
Cetinkaya
Full Professor, Department of General Surgery, Faculty of Medicine, Firat University, Elazig, Turkey. Design of the study, statistical analysis., Firat University, Department of General Surgery, Faculty of Medicine, Firat University, Elazig , Turkey.
Refik
R
Ayten
Full Professor, Department of General Surgery, Faculty of Medicine, Firat University, Elazig, Turkey. Design of the study, statistical analysis., Firat University, Department of General Surgery, Faculty of Medicine, Firat University, Elazig , Turkey.
Yavuz Selim
YS
Ilhan
Full Professor, Department of Biochemistry, Faculty of Medicine, Firat University, Elazig, Turkey. Manuscript preparation, critical revision. , Firat University, Department of Biochemistry, Faculty of Medicine, Firat University, Elazig , Turkey.
Necip
N
Ilhan
Full Professor, Department of Zootechnics, Faculty of Veterinary, Firat University, Elazig, Turkey. Critical revision, final approval., Firat University, Department of Zootechnics, Faculty of Veterinary, Firat University, Elazig , Turkey.
Ibrahim
I
Seker
Full Professor, Department of Pathology, Faculty of Veterinary, Firat University, Elazig, Turkey. Critical revision, final approval., Firat University, Department of Pathology, Faculty of Veterinary, Firat University, Elazig , Turkey.
Necati
N
Timurkaan
D000818:Animals; D003094:Collagen / Q000187:drug effects / Q000378:metabolism; D010450:Endopeptidases; D005205:Fascia / Q000098:blood supply / Q000502:physiology*; D018093:Gelatinases / Q000378:metabolism; D006909:Hydroxyproline / Q000032:analysis / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D023421:Models, Animal; D018919:Neovascularization, Physiologic; D010538:Peritonitis / Q000150:complications* / Q000378:metabolism; D053657:Platelet-Rich Plasma; D011897:Random Allocation; D017208:Rats, Wistar; D012697:Serine Endopeptidases / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000032:analysis / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000378:metabolism; D014945:Wound Healing; D000099167:Fibroblast Activation Protein Alpha
D003094:Collagen; D010450:Endopeptidases; D018093:Gelatinases; D006909:Hydroxyproline; D008565:Membrane Proteins; D012697:Serine Endopeptidases; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D000099167:Fibroblast Activation Protein Alpha