Sphingobacterium populi sp. nov., isolated from bark of Populus × euramericana.
A Gram-stain-negative, aerobic, non-motile bacterial strain, 7Y-4T, was isolated from bark tissue of Populus × euramericana. The isolate was able to grow between 10 and 37 °C, with optimal growth occurring at 28-30 °C. Strain 7Y-4T was positive for oxidase and catalase activities, but did not reduce nitrite from nitrate. Positive reactions were observed for the activities of β-galactosidase, urease and β-glucosidase, but negative reactions for the activities of gelatinase and the production of indole, acetoin and H2S. Citrate was not utilized. The major fatty acids of strain 7Y-4T are iso-C15 : 0 (28.6 %), C16 : 1ω7c/C16 : 1ω6c (31.8 %) and iso-C17 : 0 3-OH (23.3 %).The major polar lipids of the novel isolate include phosphatidylethanolamine, three unknown phospholipids (PL1-3) and six unknown lipids (L1-6), and the predominant menaquinone is MK-7. The DNA G+C content is 41.7 mol%. Analysis of 16S rRNA gene sequences revealed that the novel isolate shared the greatest sequence similarity with Sphingobacterium hotanense XH4T (93.50 %). On the basis of phenotypic and genotypic characteristics, strain 7Y-4T represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium populi is proposed. The type strain is 7Y-4T (=CFCC 11742T=KCTC 42247T).
The Key Laboratory of State Forestry Administration on Forest Protection, Research Institute of Forest Ecology Environment and Protection, Chinese Academy of Forestry, Beijing 100091, PR China.
Yong
Y
Li
The Key Laboratory of Integrated Crop Pest Management of Shandong Province, College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao 266109, P. R. China.
Li-Min
LM
Song
The Key Laboratory of State Forestry Administration on Forest Protection, Research Institute of Forest Ecology Environment and Protection, Chinese Academy of Forestry, Beijing 100091, PR China.
Min-Wei
MW
Guo
The Key Laboratory of State Forestry Administration on Forest Protection, Research Institute of Forest Ecology Environment and Protection, Chinese Academy of Forestry, Beijing 100091, PR China.
Lai-Fa
LF
Wang
The Key Laboratory of Integrated Crop Pest Management of Shandong Province, College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao 266109, P. R. China.
Wen-Xing
WX
Liang
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D024301:Plant Bark / Q000382:microbiology; D032107:Populus / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D045249:Sphingobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7