Traditional Chinese Medicine Formula "Xiaofeng granules" suppressed gouty arthritis animal models and inhibited the proteoglycan degradation on chondrocytes induced by monosodium urate.
BACKGROUND
Xiaofeng Granules (XF) is a kind of granules prepared by the famous traditional Chinese medicine formula for its efficiency in treating gouty diseases.


OBJECTIVE
We investigated the relevance between XF that made from Modified simiaowan (MSW) as the anti-gouty arthritis drugs and protective mechanisms for cartilage matrix in order to provide the evidence for new drug application.


METHODS
In the present study, we evaluated the anti-gouty arthritis activity of XF in rats and rabbits models induced by MSU together with chondrocytes focusing on the link to proteoglycan degradation in vitro studies.


RESULTS
The results demonstrated that XF significantly reduced the swelling rate and attenuated the pathological changes in joints. The XF-containing serum were used medicated serum in cellular experiments. The in vitro data were in accordance with the in vivo results, showing that the constituents in XF-containing serum had obvious inhibitory effects on the activation of pro-inflammatory mediators in chondrocytes. Moreover, XF-containing serum substantially inhibited MSU-induced expression of glycosaminoglycans(GAG) and hydroxyproline(Hyp), and up regulated proteoglycan, which might be associated with the regulation of the balance of MMP-3/TIMP-1and ADAMTS-4/TIMP-3 inchondrocytes.


CONCLUSIONS
In conclusion, XF that made from MSW showed obvious effects on acute gouty arthritis, which also provided an effective protection on cartilage matrix degradation.
College of Pharmacy, Nanjing University of Traditional Chinese Medicine, Nanjing 210023 PR China.
Le
L
Shi
College of Pharmacy, Nanjing University of Traditional Chinese Medicine, Nanjing 210023 PR China.
Fangli
F
Zhao
College of Pharmacy, Nanjing University of Traditional Chinese Medicine, Nanjing 210023 PR China.
Fangfang
F
Zhu
College of Pharmacy, Nanjing University of Traditional Chinese Medicine, Nanjing 210023 PR China.
Yuqiong
Y
Liang
College of Pharmacy, Nanjing University of Traditional Chinese Medicine, Nanjing 210023 PR China.
Fan
F
Yang
College of Pharmacy, Nanjing University of Traditional Chinese Medicine, Nanjing 210023 PR China.
Guangji
G
Zhang
College of Pharmacy, Nanjing University of Traditional Chinese Medicine, Nanjing 210023 PR China. Electronic address: xuliglp@126.com.
Li
L
Xu
College of Pharmacy, Nanjing University of Traditional Chinese Medicine, Nanjing 210023 PR China. Electronic address: yinlian162@163.com.
Lian
L
Yin
D000071121:ADAMTS4 Protein / Q000378:metabolism; D000818:Animals; D015210:Arthritis, Gouty / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects* / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006025:Glycosaminoglycans / Q000378:metabolism; D006074:Gout Suppressants / Q000494:pharmacology*; D006909:Hydroxyproline / Q000378:metabolism; D018836:Inflammation Mediators / Q000378:metabolism; D007596:Joints / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D019278:Matrix Metalloproteinase 3 / Q000378:metabolism; D011509:Proteoglycans / Q000378:metabolism*; D059748:Proteolysis; D011817:Rabbits; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism; D019717:Tissue Inhibitor of Metalloproteinase-3 / Q000378:metabolism; D014527:Uric Acid
D016207:Cytokines; D004365:Drugs, Chinese Herbal; D006025:Glycosaminoglycans; D006074:Gout Suppressants; D018836:Inflammation Mediators; D011509:Proteoglycans; C576839:TIMP1 protein, rat; D019715:Tissue Inhibitor of Metalloproteinase-1; D019717:Tissue Inhibitor of Metalloproteinase-3; C000617451:xiaofeng granules; D014527:Uric Acid; C571365:ADAMTS4 protein, rat; D019278:Matrix Metalloproteinase 3; D000071121:ADAMTS4 Protein; D006909:Hydroxyproline
Chondrocytes; Gouty arthritis; Monosodium urate; Proteoglycan; Xiaofeng granules