A Fluorescent Polymer Probe with High Selectivity toward Vascular Endothelial Cells for and beyond Noninvasive Two-Photon Intravital Imaging of Brain Vasculature.
A chromophore-engineering strategy that relies on the introduction of a ground-state distortion in a quadrupolar chromophore was used to obtain a quasi-quadrupolar chromophore with red emission and large two-photon absorption (2PA) cross-section in polar solvents. This molecule was functionalized with water-solubilizing polymer chains. It constitutes not only a remarkable contrast agent for intravital two-photon microscopy of the functional cerebral vasculature in a minimally invasive configuration but presents intriguing endothelial staining ability that makes it a valuable probe for premortem histological staining.
Laboratoire de Chimie, ENS de Lyon, CNRS UMR 5182, Université Claude Bernard, Université de Lyon , F69342, Lyon, France.
B
B
Mettra
Grenoble Institut des Neurosciences, GIN, Inserm, U1216, Univ Grenoble Alpes , F-38000 Grenoble, France.
F
F
Appaix
Advanced Materials Engineering and Modelling Group, Faculty of Chemistry, Wroclaw University of Technology , Wyb. Wyspianskiego 27, 50-370 Wroclaw, Poland.
J
J
Olesiak-Banska
Laboratoire de Chimie, ENS de Lyon, CNRS UMR 5182, Université Claude Bernard, Université de Lyon , F69342, Lyon, France.
T
T
Le Bahers
Laboratoire de Chimie, ENS de Lyon, CNRS UMR 5182, Université Claude Bernard, Université de Lyon , F69342, Lyon, France.
A
A
Leung
Advanced Materials Engineering and Modelling Group, Faculty of Chemistry, Wroclaw University of Technology , Wyb. Wyspianskiego 27, 50-370 Wroclaw, Poland.
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K
Matczyszyn
Advanced Materials Engineering and Modelling Group, Faculty of Chemistry, Wroclaw University of Technology , Wyb. Wyspianskiego 27, 50-370 Wroclaw, Poland.
M
M
Samoc
CLINATEC, INSERM UA 01, Rue des Martyrs 17, 38054, Grenoble, France.
B
B
van der Sanden
Laboratoire de Chimie, ENS de Lyon, CNRS UMR 5182, Université Claude Bernard, Université de Lyon , F69342, Lyon, France.
C
C
Monnereau
Laboratoire de Chimie, ENS de Lyon, CNRS UMR 5182, Université Claude Bernard, Université de Lyon , F69342, Lyon, France.
C
C
Andraud
D001921:Brain / Q000098:blood supply*; D042783:Endothelial Cells; D005456:Fluorescent Dyes; D000069416:Intravital Microscopy; D017785:Photons; D011108:Polymers
D005456:Fluorescent Dyes; D011108:Polymers
TD-DFT; fluorescence; intravital microscopy; near infrared; star-shaped polymer; two-photon absorption