Single-Step Multiplex PCR Assay for Determining 92 Pneumococcal Serotypes.
For pneumococcal disease surveillance, simple and cost-effective methods capable of determining all serotypes are needed. Combining a single-tube multiplex PCR with fluorescently labeled primers followed by amplicon analysis using automated fluorescent capillary electrophoresis, each serotype of 92 reference isolates and 297 recently collected clinical isolates was successfully determined.
Microbiology Department, Hospital Universitario Donostia-Instituto de Investigación Sanitaria Biodonostia, San Sebastián, Spain Biomedical Research Center Network for Respiratory Diseases (CIBERES), Madrid, Spain josemaria.marimonortizdez@osakidetza.eus.
José M
JM
Marimón
Biomedical Research Center Network for Respiratory Diseases (CIBERES), Madrid, Spain.
María
M
Ercibengoa
Microbiology and Virology Unit, Padua University Hospital, Padua, Italy.
Erica
E
Santacatterina
Microbiology Department, Hospital Universitario Donostia-Instituto de Investigación Sanitaria Biodonostia, San Sebastián, Spain.
Marta
M
Alonso
Microbiology Department, Hospital Universitario Donostia-Instituto de Investigación Sanitaria Biodonostia, San Sebastián, Spain Biomedical Research Center Network for Respiratory Diseases (CIBERES), Madrid, Spain Faculty of Medicine, University of the Basque Country, UPV/EHU, San Sebastián, Spain.
Emilio
E
Pérez-Trallero
D006801:Humans; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D065288:Serogroup; D012703:Serotyping / Q000379:methods*; D013296:Streptococcus pneumoniae / Q000145:classification* / Q000235:genetics*