Pulmonary and pleural inflammation after intratracheal instillation of short single-walled and multi-walled carbon nanotubes.
Relationships between the physical properties of carbon nanotubes (CNTs) and their toxicities have been studied. However, little research has been conducted to investigate the pulmonary and pleural inflammation caused by short-fiber single-walled CNTs (SWCNTs) and multi-walled CNTs (MWCNTs). This study was performed to characterize differences in rat pulmonary and pleural inflammation caused by intratracheal instillation with doses of 0.15 or 1.5mg/kg of either short-sized SWCNTs or MWCNTs. Data from bronchoalveolar lavage fluid analysis, histopathological findings, and transcriptional profiling of rat lungs obtained over a 90-day period indicated that short SWCNTs caused persistent pulmonary inflammation. In addition, the short MWCNTs markedly impacted alveoli immediately after instillation, with the levels of pulmonary inflammation following MWCNT instillation being reduced in a time-dependent manner. MWCNT instillation induced greater levels of pleural inflammation than did short SWCNTs. SWCNTs and MWCNTs translocated in mediastinal lymph nodes were observed, suggesting that SWCNTs and MWCNTs underwent lymphatic drainage to the mediastinal lymph nodes after pleural penetration. Our results suggest that short SWCNTs and MWCNTs induced pulmonary and pleural inflammation and that they might be transported throughout the body after intratracheal instillation. The extent of changes in inflammation differed following SWCNT and MWCNT instillation in a time-dependent manner.
Research Institute of Science for Safety and Sustainability (RISS), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8569, Japan; Technology Research Association for Single Wall Carbon Nanotubes (TASC), Tsukuba, Ibaraki 305-8565, Japan,. Electronic address: ka-fujita@aist.go.jp.
Katsuhide
K
Fujita
Technology Research Association for Single Wall Carbon Nanotubes (TASC), Tsukuba, Ibaraki 305-8565, Japan.
Makiko
M
Fukuda
Technology Research Association for Single Wall Carbon Nanotubes (TASC), Tsukuba, Ibaraki 305-8565, Japan.
Shigehisa
S
Endoh
Technology Research Association for Single Wall Carbon Nanotubes (TASC), Tsukuba, Ibaraki 305-8565, Japan.
Junko
J
Maru
Technology Research Association for Single Wall Carbon Nanotubes (TASC), Tsukuba, Ibaraki 305-8565, Japan,; National Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8565, Japan.
Haruhisa
H
Kato
National Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8565, Japan.
Ayako
A
Nakamura
Research Institute of Science for Safety and Sustainability (RISS), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8569, Japan; Technology Research Association for Single Wall Carbon Nanotubes (TASC), Tsukuba, Ibaraki 305-8565, Japan.
Naohide
N
Shinohara
Technology Research Association for Single Wall Carbon Nanotubes (TASC), Tsukuba, Ibaraki 305-8565, Japan.
Kanako
K
Uchino
Research Institute of Science for Safety and Sustainability (RISS), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8569, Japan; Technology Research Association for Single Wall Carbon Nanotubes (TASC), Tsukuba, Ibaraki 305-8565, Japan.
Kazumasa
K
Honda
D000818:Animals; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D018836:Inflammation Mediators / Q000378:metabolism; D019570:Inhalation Exposure; D008168:Lung / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008208:Lymphatic System / Q000187:drug effects / Q000378:metabolism; D008297:Male; D037742:Nanotubes, Carbon / Q000633:toxicity*; D010994:Pleura / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D010998:Pleurisy / Q000139:chemically induced* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D011014:Pneumonia / Q000139:chemically induced* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D017208:Rats, Wistar; D013997:Time Factors
D016207:Cytokines; D018836:Inflammation Mediators; D037742:Nanotubes, Carbon
Intratracheal instillation; Multi-walled carbon nanotubes (MWCNTs); Pleural inflammation; Pulmonary inflammation; Single-walled carbon nanotubes (SWCNTs)