Experiment research of cisplatin implants inhibiting transplantation tumor growth and regulating the expression of KLK7 and E-cad of tumor-bearing mice with gastric cancer.
OBJECTIVE
To study the influence of cisplatin implants on transplantation tumor growth and the expression of tissue kallikrein-7 (KLK7) and E-cadherin (E-cad) in tumor-bearing mice with gastric cancer.


METHODS
BALB/c nude mice were collected as experimental animal and were randomly divided into model control group (Group A), tail intravenous injection of cisplatin group (Group B), intratumor injection of cisplatin group (Group C) and cisplatin implants treatment group (Group D). After the drugs intervening, the weight and volume of transplantation tumors were measured on Day 20, Day 30 and Day 40 and serum and KLK7 and E-cad contents in transplanted tumor tissue were examined.


RESULTS
On Day 20, Day 30 and Day 40 after treatment, the weight and volume of transplantation tumors of tumor-bearing mice in four groups were different (Group A > Group B > Group C > Group D). The contents of KLK-7 and E-cad in tumor tissue and serum of tumor-bearing mice in four groups were different (Group A > Group B > Group C > Group D in KLK-7) and (Group A < Group B < Group C < Group D in E-cad). The weight and volume, and KLK7 and E-cad contents of transplantation tumors in four groups were significant difference (P < 0.05).


CONCLUSIONS
Cisplatin implants can inhibit the growth of transplanted tumor tissue and down-regulated KLK7 expression and up-regulated E-cad expression of tumor-bearing mice with gastric cancer.
Department of Digestive System, Affiliated Hospital of Hainan Medical College, Haikou 570102, China.
Gui-Feng
GF
Zhuang
Department of Digestive System, Affiliated Hospital of Hainan Medical College, Haikou 570102, China.
Yan
Y
Tan
Department of Digestive System, Affiliated Hospital of Hainan Medical College, Haikou 570102, China. Electronic address: hhyangyuanzheng@163.com.
Yuan-Zheng
YZ
Yang
Department of Digestive System, Affiliated Hospital of Hainan Medical College, Haikou 570102, China.
Jie-Wei
JW
Zhang
Department of Digestive System, Affiliated Hospital of Hainan Medical College, Haikou 570102, China.
Jing
J
Tang
Cisplatin implants; E-cadherin; Gastric cancer; Tissue kallikrein-7