ACA-Pro: calibration protocol for quantitative diffuse reflectance spectroscopy. Validation on contact and noncontact probe- and CCD-based systems.
We have developed an adaptive calibration algorithm and protocol (ACA-Pro) that corrects from the instrumental response of various spatially resolved diffuse reflectance spectroscopy (DRSsr) systems to enable the quantification of absorption and scattering properties based on a Monte Carlo-based look-up-table approach. The protocol involves the use of a calibration reference base built with measurements of a range of different diffusive intralipid phantoms. Moreover, an advanced strategy was established to take into account the experimental variations with an additional measurement of a common solid material, allowing the use of a single calibration reference base for all experiments. The ACA-Pro is validated in contact and noncontact probe-based DRSsr systems. Furthermore, the first results of a setup replacing the probe with a CCD detector are shown to confirm the robustness of the approach.
CEA-LETI, Minatec Campus, 17 Rue des Martyrs, F38054 Grenoble, Cedex 9, France.
Veronica
V
Sorgato
CEA-LETI, Minatec Campus, 17 Rue des Martyrs, F38054 Grenoble, Cedex 9, France.
Michel
M
Berger
CEA-LETI, Minatec Campus, 17 Rue des Martyrs, F38054 Grenoble, Cedex 9, France.
Charlotte
C
Emain
UPMC University Paris 06, UMR8237, Laboratoire Jean Perrin, F-75005, Paris, FrancecCNRS, UMR8237, Laboratoire Jean Perrin, F-75005, Paris, France.
Christine
C
Vever-Bizet
CEA-LETI, Minatec Campus, 17 Rue des Martyrs, F38054 Grenoble, Cedex 9, France.
Jean-Marc
JM
Dinten
UPMC University Paris 06, UMR8237, Laboratoire Jean Perrin, F-75005, Paris, FrancecCNRS, UMR8237, Laboratoire Jean Perrin, F-75005, Paris, France.
Geneviève
G
Bourg-Heckly
CEA-LETI, Minatec Campus, 17 Rue des Martyrs, F38054 Grenoble, Cedex 9, France.
Anne
A
Planat-Chrétien
D000465:Algorithms; D002138:Calibration; D004058:Diffusion; D005456:Fluorescent Dyes; D009010:Monte Carlo Method; D019047:Phantoms, Imaging; D013057:Spectrum Analysis / Q000379:methods*
D005456:Fluorescent Dyes