Changes in the Arabidopsis thaliana Proteome Implicate cAMP in Biotic and Abiotic Stress Responses and Changes in Energy Metabolism.
The second messenger 3',5'-cyclic adenosine monophosphate (cAMP) is increasingly recognized as having many different roles in plant responses to environmental stimuli. To gain further insights into these roles, Arabidopsis thaliana cell suspension culture was treated with 100 nM of cell permeant 8-bromo-cAMP for 5 or 10 min. Here, applying mass spectrometry and comparative proteomics, 20 proteins were identified as differentially expressed and we noted a specific bias in proteins with a role in abiotic stress, particularly cold and salinity, biotic stress as well as proteins with a role in glycolysis. These findings suggest that cAMP is sufficient to elicit specific stress responses that may in turn induce complex changes to cellular energy homeostasis.
Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia. may.qurashi@kaust.edu.sa.|Cambridge Centre for Proteomics, Cambridge System Biology, Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK. may.qurashi@kaust.edu.sa.
May
M
Alqurashi
Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia. christoph.gehring@kaust.edu.sa.
Chris
C
Gehring
Cambridge Centre for Proteomics, Cambridge System Biology, Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK. cm833@cam.ac.uk.
Claudius
C
Marondedze
D017360:Arabidopsis / Q000378:metabolism*; D002952:Citric Acid Cycle; D000242:Cyclic AMP / Q000378:metabolism*; D004734:Energy Metabolism; D006019:Glycolysis; D010101:Oxygen Consumption; D020543:Proteome; D040901:Proteomics / Q000379:methods; D015398:Signal Transduction; D013312:Stress, Physiological
D020543:Proteome; D000242:Cyclic AMP
TCA cycle; abiotic stress; biotic stress; cAMP-dependent proteome; glycolysis