Qualitative and quantitative determination of R-phycoerythrin from Halymenia floresia (Clemente) C. Agardh by polyacrylamide gel using electrophoretic elution technique.
R-Phycoerythrin is one of the phycobiliproteins widely found in seaweeds. In this study, we have shown to extract and purify R-Phycoerythrin from the south east cost Indian red seaweed Halymenia floresia. R-Phycoerythrin was extracted in 50mM phosphate buffer (pH 7.0). The preparative native PAGE purification was employed alternative to the chromatography and therefore can be scaled up efficiently. Both the yield and the purity of R-Phycoerythrin are very effective. The purified R-Phycoerythrin showed a single band on the examination by native PAGE electrophrosis. SDS-PAGE analysis showed five bands at 16kDa, 21kDa, 30kDa, 39kDa and 47kDa which corresponds to the α, β and γ', γ and αβ subunits. This preparative method for R-Phycoerythrin purification can offer a reference for R-Phycoerythrin purification from other marine red macro algae.
Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai 600 025, Tamilnadu, India. Electronic address: sathuvansjc@gmail.com.
Sathuvan
S
Malairaj
Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai 600 025, Tamilnadu, India.
Sakthivel
S
Muthu
Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai 600 025, Tamilnadu, India.
Venkatesh Babu
VB
Gopal
Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai 600 025, Tamilnadu, India.
Palani
P
Perumal
Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai 600 025, Tamilnadu, India.
Rengasamy
R
Ramasamy
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D010799:Phycoerythrin / Q000032:analysis* / Q000737:chemistry*; D000461:Rhodophyta / Q000737:chemistry*
D010799:Phycoerythrin
Electrophoretic elution; Halymenia floresia; Preparative native PAGE; R-phycoerythrin