KRAS Mutations in Canine and Feline Pancreatic Acinar Cell Carcinoma.
Companion animals may serve as valuable models for studying human cancers. Although KRAS is the most commonly mutated gene in human ductal pancreatic cancers (57%), with mutations frequently occurring at codons 12, 13 and 61, human pancreatic acinar cell carcinomas (ACCs) lack activating KRAS mutations. In the present study, 32 pancreatic ACC samples obtained from 14 dogs and 18 cats, including seven metastases, were analyzed for six common activating KRAS mutations located in codons 12 (n = 5) and 13 (n = 1) using Sequenom MassARRAY. No KRAS mutations were found, suggesting that, similar to human pancreatic ACC, KRAS mutations do not play a critical role in feline or canine pancreatic ACC. Due to the similarity of the clinical disease in dogs and cats to that of man, this study confirms that companion animals offer potential as a suitable model for investigating this rare subtype of pancreatic carcinoma.
Transformative Pathology Platform, Ontario Institute for Cancer Research, Toronto, Ontario, Canada.
C
C
Crozier
Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
G A
GA
Wood
Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
R A
RA
Foster
Transformative Pathology Platform, Ontario Institute for Cancer Research, Toronto, Ontario, Canada.
S
S
Stasi
Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
J H W
JH
Liu
Transformative Pathology Platform, Ontario Institute for Cancer Research, Toronto, Ontario, Canada.
J M S
JM
Bartlett
Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada. Electronic address: bcoomber@uoguelph.ca.
B L
BL
Coomber
Transformative Pathology Platform, Ontario Institute for Cancer Research, Toronto, Ontario, Canada.
V S
VS
Sabine
D000818:Animals; D002371:Cat Diseases / Q000235:genetics*; D002415:Cats; D004252:DNA Mutational Analysis; D004283:Dog Diseases / Q000235:genetics*; D004285:Dogs; D060885:Multiplex Polymerase Chain Reaction; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D010190:Pancreatic Neoplasms / Q000662:veterinary*; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics*
D016283:Proto-Oncogene Proteins p21(ras)
KRAS mutation; companion animal; model; pancreatic acinar cell carcinoma