Maleic acid treatment of biologically detoxified corn stover liquor.
Elimination of microbial and enzyme inhibitors from pretreated lignocellulose is critical for effective cellulose conversion and yeast fermentation of liquid hot water (LHW) pretreated corn stover. In this study, xylan oligomers were hydrolyzed using either maleic acid or hemicellulases, and other soluble inhibitors were eliminated by biological detoxification. Corn stover at 20% (w/v) solids was LHW pretreated LHW (severity factor: 4.3). The 20% solids (w/v) pretreated corn stover derived liquor was recovered and biologically detoxified using the fungus Coniochaeta ligniaria NRRL30616. After maleic acid treatment, and using 5 filter paper units of cellulase/g glucan (8.3mg protein/g glucan), 73% higher cellulose conversion from corn stover was obtained for biodetoxified samples compared to undetoxified samples. This corresponded to 87% cellulose to glucose conversion. Ethanol production by yeast of pretreated corn stover solids hydrolysate was 1.4 times higher than undetoxified samples, with a reduction of 3h in the fermentation lag phase.
Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, IN 47907-2032, United States; Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907-2032, United States.
Daehwan
D
Kim
Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, IN 47907-2032, United States; Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907-2032, United States.
Eduardo A
EA
Ximenes
Bioenergy Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, USDA, 1815 N. University Street, Peoria, IL 61604, United States.
Nancy N
NN
Nichols
School of Life Science and Technology, Harbin Institute of Technology, Harbin 150001, China; State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150001, China.
Guangli
G
Cao
Bioenergy Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, USDA, 1815 N. University Street, Peoria, IL 61604, United States.
Sarah E
SE
Frazer
Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, IN 47907-2032, United States; Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907-2032, United States; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907-2032, United States. Electronic address: ladisch@purdue.edu.
Michael R
MR
Ladisch
D001203:Ascomycota / Q000378:metabolism; D019149:Bioreactors; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism; D000431:Ethanol / Q000138:chemical synthesis*; D005285:Fermentation; D006868:Hydrolysis; D008658:Inactivation, Metabolic; D008298:Maleates / Q000737:chemistry*; D003313:Zea mays / Q000737:chemistry / Q000378:metabolism
D008298:Maleates; D000431:Ethanol; D002482:Cellulose; C030272:maleic acid
Bioabatement; Enzymatic hydrolysis; Ethanol; Fermentation; Hemicellulase supplementation; Inhibitors; Liquid hot water pretreatment; Maleic acid