Structural characterization and antidiabetic activity of a glucopyranose-rich heteropolysaccharide from Catathelasma ventricosum.
Polysaccharides, which are the main bioactive constituents of edible mushrooms, have been shown to have a variety of useful biological activities. In this study, a polysaccharide fraction from the edible mushroom Catathelasma ventricosum was purified using anion exchange and size exclusion chromatographies. The structure of the resulting polysaccharide, named CVP-1S, was characterized on the basis of partial acid hydrolysis, periodic acid oxidation, methylation analysis, gas chromatography-mass spectrometry, Fourier-transform infrared spectroscopy, 1D/2D NMR spectroscopy, scanning electron microscopy, and atomic force spectroscopy. The results showed that CVP-1S is a heteropolysaccharide consisting of glucose (94.2%), galactose (3.51%) and fucose (1.3%) with a molecular weight of 1.5×10(4)Da. Its backbone is mainly linked by (1→6)-β-d-Glcp glycosidic bonds, and branches are attached to the backbone through 1,3-linked glycosidic bonds. CVP-1S was also found to have antioxidant, hypoglycemic, and hypolipidemic activities in the streptozoicin-induced diabetic mouse model. From these results, we conclude that CVP-1S should receive further attention as a potential agent for the treatment and prevention of diabetes.
Key Laboratory of Animal Disease-Resistance Nutrition, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China; College of Food Science, Sichuan Agricultural University, Yaan 625014, China.
Yuntao
Y
Liu
College of Food Science, Sichuan Agricultural University, Yaan 625014, China.
Di
D
Chen
College of Food Science, Sichuan Agricultural University, Yaan 625014, China.
Yuxian
Y
You
College of Food Science, Sichuan Agricultural University, Yaan 625014, China.
Siqi
S
Zeng
College of Food Science, Sichuan Agricultural University, Yaan 625014, China.
Yu
Y
Hu
College of Food Science, Sichuan Agricultural University, Yaan 625014, China.
Xiaoyu
X
Duan
College of Food Science, Sichuan Agricultural University, Yaan 625014, China.
Aiping
A
Liu
College of Food Science, Sichuan Agricultural University, Yaan 625014, China.
Hong
H
Chen
College of Food Science, Sichuan Agricultural University, Yaan 625014, China.
Xinjie
X
Hu
College of Food Science, Sichuan Agricultural University, Yaan 625014, China.
Shujuan
S
Chen
College of Food Science, Sichuan Agricultural University, Yaan 625014, China.
Cheng
C
Li
Key Laboratory of Animal Disease-Resistance Nutrition, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China. Electronic address: dwchen@sicau.edu.cn.
Daiwen
D
Chen
D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000188:drug therapy / Q000378:metabolism; D005947:Glucose / Q000737:chemistry*; D006003:Glycogen / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000737:chemistry* / Q000494:pharmacology* / Q000627:therapeutic use; D007328:Insulin / Q000097:blood; D008055:Lipids / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology* / Q000627:therapeutic use
D001786:Blood Glucose; D007004:Hypoglycemic Agents; D007328:Insulin; D008055:Lipids; D011134:Polysaccharides; D006003:Glycogen; D005947:Glucose
Antidiabetic acticity; Catathelasma ventricosum; Polysaccharide; Structure characterization