Neuroprotection of Grape Seed Extract and Pyridoxine against Triton-Induced Neurotoxicity.
Triton WR-1339 administration causes neurotoxicity. Natural products and herbal extracts can attenuate cerebral injury. In the present study, we investigated the neuroprotective role of grape seed extract and/or vitamin B6 against triton-induced neurotoxicity. Thirty-five adult male albino rats of the Sprague-Dawley strain, weighing 140-145 g, were divided into five groups: control, triton, grape seed extract + triton, grape seed extract + triton + vitamin B6, and vitamin B6 + triton. The hematological and biochemical analyses were carried out. Alteration in iNOS mRNA gene expression was determined using reverse-transcriptase PCR analysis. In addition, qualitative DNA fragmentation was examined using agarose gel electrophoresis. Triton-treatment caused significant disturbances in the hematological parameters, the neurological functions, and the antioxidant profile. Also, triton significantly increased the iNOS mRNA expression and DNA damage. Our results showed that grape seed extract and/or vitamin B6 could attenuate all the examined parameters. These natural substances could exhibit protective effects against triton-induced neurological damage because of their antioxidative and antiapoptotic capacities.
Faculty of Science, Zoology Department, Alexandria University, Alexandria 21311, Egypt.
Heba M
HM
Abdou
Faculty of Science, Biochemistry Department, Alexandria University, Alexandria 21311, Egypt.
Mayssaa M
0000-0003-0765-8967
MM
Wahby
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D019610:Cytoprotection; D053938:DNA Fragmentation; D004195:Disease Models, Animal; D004359:Drug Therapy, Combination; D004587:Electrophoresis, Agar Gel; D056604:Grape Seed Extract / Q000494:pharmacology*; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology*; D020258:Neurotoxicity Syndromes / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D011092:Polyethylene Glycols; D011736:Pyridoxine / Q000494:pharmacology*; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000975:Antioxidants; D056604:Grape Seed Extract; D018696:Neuroprotective Agents; D011092:Polyethylene Glycols; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; D011736:Pyridoxine; C016811:tyloxapol