Biogenic terbium oxide nanoparticles as the vanguard against osteosarcoma.
The synthesis of inner transition metal nanoparticles via an ecofriendly route is quite difficult. This study, for the first time, reports synthesis of terbium oxide nanoparticles using fungus, Fusarium oxysporum. The biocompatible terbium oxide nanoparticles (Tb2O3 NPs) were synthesized by incubating Tb4O7 with the biomass of fungus F. oxysporum. Multiple physical characterization techniques, such as UV-visible and photoluminescence spectroscopy, TEM, SAED, and zeta-potential were used to confirm the synthesis, purity, optical and surface characteristics, crystallinity, size, shape, distribution, and stability of the nanoemulsion of Tb2O3 NPs. The Tb2O3 NPs were found to inhibit the propagation of MG-63 and Saos-2 cell-lines (IC50 value of 0.102μg/mL) and remained non-toxic up to a concentration of 0.373μg/mL toward primary osteoblasts. Cell viability decreased in a concentration-dependent manner upon exposure to 10nm Tb2O3 NPs in the concentration range 0.023-0.373μg/mL. Cell toxicity was evaluated by observing changes in cell morphology, cell viability, oxidative stress parameters, and FACS analysis. Morphological examinations of cells revealed cell shrinkage, nuclear condensation, and formation of apoptotic bodies. The level of ROS within the cells-an indicator of oxidative stress was significantly increased. The induction of apoptosis at concentrations ≤IC50 was corroborated by 4',6-diamidino-2-phenylindole dihydrochloride (DAPI) staining (DNA damage and nuclear fragmentation). Flow-cytometric studies indicated that the response was dose dependent with a threshold effect.
Department of Biotechnology, Integral University, Lucknow, Uttar Pradesh 226026, India.
Sana
S
Iram
Department of Biotechnology, Integral University, Lucknow, Uttar Pradesh 226026, India.
Salman
S
Khan
Biochemical Sciences Division, National Chemical Laboratory, Pune 411008, India.
Abu Ayoobul
AA
Ansary
Molecular Endocrinology Lab, Department of Zoology, Lucknow University, Lucknow 226025, India.
Mohd
M
Arshad
Molecular Endocrinology Lab, Department of Zoology, Lucknow University, Lucknow 226025, India.
Sahabjada
S
Siddiqui
IBU, Aligarh Muslim University, Aligarh 202002, India.
Ejaz
E
Ahmad
IBU, Aligarh Muslim University, Aligarh 202002, India.
Rizwan H
RH
Khan
Department of Biotechnology, Integral University, Lucknow, Uttar Pradesh 226026, India. Electronic address: research.sajid@gmail.com.
Mohd Sajid
MS
Khan
D001859:Bone Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005670:Fusarium / Q000737:chemistry; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D036103:Nanotechnology; D012516:Osteosarcoma / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D010087:Oxides / Q000737:chemistry / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013725:Terbium / Q000737:chemistry / Q000494:pharmacology*
D010087:Oxides; D017382:Reactive Oxygen Species; D013725:Terbium
Biosynthesis; Cytotoxicity; Fusarium oxysporum; Osteosarcoma; Tb(2)O(3) nanoparticles