High-contrast grating resonators for label-free detection of disease biomarkers.
A label-free optical biosensor is described that employs a silicon-based high-contrast grating (HCG) resonator with a spectral linewidth of ~500 pm that is sensitive to ligand-induced changes in surface properties. The device is used to generate thermodynamic and kinetic data on surface-attached antibodies with their respective antigens. The device can detect serum cardiac troponin I, a biomarker of cardiac disease to 100 pg/ml within 4 mins, which is faster, and as sensitive as current enzyme-linked immuno-assays for cTnI.
Department of Electrical Engineering and Computer Science, University of California, Berkeley, CA 94720, USA.
Tianbo
T
Sun
Department of Bioengineering, University of California, Berkeley, CA 94720, USA.
Shu
S
Kan
Department of Bioengineering, University of California, Berkeley, CA 94720, USA.
Gerard
G
Marriott
Department of Electrical Engineering and Computer Science, University of California, Berkeley, CA 94720, USA.
Connie
C
Chang-Hasnain
D015415:Biomarkers / Q000378:metabolism*; D015374:Biosensing Techniques; D006331:Heart Diseases / Q000097:blood; D006801:Humans; D007700:Kinetics; D008024:Ligands; D012680:Sensitivity and Specificity; D013499:Surface Properties; D013816:Thermodynamics; D019210:Troponin I / Q000097:blood
D015415:Biomarkers; D008024:Ligands; D019210:Troponin I