Identification of a Novel Gibberellin (GA85) in Very Young Seedlings of Brassica campestris cv. Tobin.
A new gibberellin (GA) was identified from extracts of cotyledons of 7 day-old canola seedlings (Brassica campestris cv. Tobin). This GA is 12α-hydroxy-GA1 and has been assigned the trivial name of GA85. Isolation was monitored by the Tan-ginbozu dwarf rice micro-drop assay after each high-performance liquid chromatography (HPLC) step. Identification was based on Kovats retention index (KRI) and the mass spectrum of the methyl ester, trimethylsilyl ether (MeTMSi) derivative after analysis by gas chromatography-mass spectrometry (GC-MS) in comparison with an authentic sample of 12α-hydroxy-GA1. Based on quantitation by the dwarf rice micro-drop assay, GA85 is one of the major biologically active GAs in cotyledons of young canola seedlings.
a Plant Physiology Research Group, Department of Biological Sciences , The University of Calgary , Calgary , Alberta , Canada T2N 1N4.
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a Plant Physiology Research Group, Department of Biological Sciences , The University of Calgary , Calgary , Alberta , Canada T2N 1N4.|b Research School of Chemistry, The Australian National University , G.P.O. Box 4 , Canberra , A.C.T. 2601 , Australia.
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Bhaskar
a Plant Physiology Research Group, Department of Biological Sciences , The University of Calgary , Calgary , Alberta , Canada T2N 1N4.|b Research School of Chemistry, The Australian National University , G.P.O. Box 4 , Canberra , A.C.T. 2601 , Australia.
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Alexis Chu
a Plant Physiology Research Group, Department of Biological Sciences , The University of Calgary , Calgary , Alberta , Canada T2N 1N4.|b Research School of Chemistry, The Australian National University , G.P.O. Box 4 , Canberra , A.C.T. 2601 , Australia.
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Mander
a Plant Physiology Research Group, Department of Biological Sciences , The University of Calgary , Calgary , Alberta , Canada T2N 1N4.
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Pearce
a Plant Physiology Research Group, Department of Biological Sciences , The University of Calgary , Calgary , Alberta , Canada T2N 1N4.
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Pharis
a Plant Physiology Research Group, Department of Biological Sciences , The University of Calgary , Calgary , Alberta , Canada T2N 1N4.|c ESSO Ag Biologicals, 402-15 Innovation Blvd., Saskatoon , Saskatchewan , Canada S7N 2X8.
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