A label-free colorimetric aptasensor for simple, sensitive and selective detection of Pt (II) based on platinum (II)-oligonucleotide coordination induced gold nanoparticles aggregation.
Herein, a gold nanoparticles (AuNPs) based label-free colorimetric aptasensor for simple, sensitive and selective detection of Pt (II) was constructed for the first time. Four bases (G-G mismatch) mismatched streptavidin aptamer (MSAA) was used to protect AuNPs from salt-induced aggregation and recognize Pt (II) specifically. Only in the presence of Pt (II), coordination occurs between G-G bases and Pt (II), leading to the activation of streptavidin aptamer. Streptavidin coated magnetic beads (MBs) were used as separation agent to separate Pt (II)-coordinated MSAA. The residual less amount of MSAA could not efficiently protect AuNPs anymore and aggregation of AuNPs will produce a colorimetric product. With the addition of Pt (II), a pale purple-to-blue color variation could be observed by the naked eye. A detection limit of 150nM and a linear range from 0.6μM to 12.5μM for Pt (II) could be achieved without any amplification.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, PR China; University of Chinese Academy of Sciences, Beijing 100039, PR China.
Daoqing
D
Fan
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, PR China; University of Chinese Academy of Sciences, Beijing 100039, PR China.
Qingfeng
Q
Zhai
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, PR China; University of Chinese Academy of Sciences, Beijing 100039, PR China.
Weijun
W
Zhou
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, PR China; University of Chinese Academy of Sciences, Beijing 100039, PR China.
Xiaoqing
X
Zhu
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, PR China; University of Chinese Academy of Sciences, Beijing 100039, PR China.
Erkang
E
Wang
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, PR China; University of Chinese Academy of Sciences, Beijing 100039, PR China. Electronic address: dongsj@ciac.ac.cn.
Shaojun
S
Dong
D000818:Animals; D000970:Antineoplastic Agents / Q000032:analysis / Q000652:urine*; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D002413:Cations, Divalent / Q000032:analysis / Q000652:urine; D002945:Cisplatin / Q000032:analysis / Q000652:urine*; D003124:Colorimetry / Q000379:methods; D006046:Gold / Q000737:chemistry*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010984:Platinum / Q000032:analysis / Q000652:urine*; D051381:Rats
D000970:Antineoplastic Agents; D052157:Aptamers, Nucleotide; D002413:Cations, Divalent; D010984:Platinum; D006046:Gold; D002945:Cisplatin
Aptamer sensor; Colorimetric; Gold nanoparticles; Platinum ion; Streptavidin