Use of In Vitro Transcription System for Analysis of Corynebacterium glutamicum Promoters Recognized by Two Sigma Factors.
Promoter activities in Corynebacterium glutamicum strains with deletions of genes encoding sigma factors of RNA polymerase suggested that transcription from some promoters is controlled by two sigma factors. To prove that different sigma factors are involved in the recognition of selected Corynebacterium glutamicum promoters, in vitro transcription system was applied. It was found that a typical housekeeping promoter Pper interacts with the alternative sigma factor σ(B) in addition to the primary sigma factor σ(A). On the other way round, the σ(B)-dependent promoter of the pqo gene that is expressed mainly in the stationary growth phase was active also with σ(A). Some promoters of genes involved in stress responses (P1clgR, P2dnaK, and P2dnaJ2) were found to be recognized by two stress-responding sigma factors, σ(H) and σ(E). In vitro transcription system thus proved to be a useful direct technique for demonstrating the overlap of different sigma factors in recognition of individual promoters in C. glutamicum.
Institute of Microbiology, Czech Academy of Sciences, v.v.i., Vídeňská 1083, 142 20, Prague, Czech Republic.
Radoslav
R
Šilar
Institute of Microbiology, Czech Academy of Sciences, v.v.i., Vídeňská 1083, 142 20, Prague, Czech Republic.
Jiří
J
Holátko
Institute of Microbiology, Czech Academy of Sciences, v.v.i., Vídeňská 1083, 142 20, Prague, Czech Republic.
Lenka
L
Rucká
Institute of Microbiology, Czech Academy of Sciences, v.v.i., Vídeňská 1083, 142 20, Prague, Czech Republic.
Andrey
A
Rapoport
Institute of Microbiology, Czech Academy of Sciences, v.v.i., Vídeňská 1083, 142 20, Prague, Czech Republic.
Hana
H
Dostálová
Institute of Microbiology, Czech Academy of Sciences, v.v.i., Vídeňská 1083, 142 20, Prague, Czech Republic.
Pavla
P
Kadeřábková
Institute of Microbiology, Czech Academy of Sciences, v.v.i., Vídeňská 1083, 142 20, Prague, Czech Republic. nesvera@biomed.cas.cz.
Jan
J
Nešvera
Institute of Microbiology, Czech Academy of Sciences, v.v.i., Vídeňská 1083, 142 20, Prague, Czech Republic.
Miroslav
M
Pátek
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D048230:Corynebacterium glutamicum / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D011401:Promoter Regions, Genetic; D012808:Sigma Factor / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D012808:Sigma Factor