Sugar Beet Pulp as Leuconostoc mesenteroides T3 Support for Enhanced Dextransucrase Production on Molasses.
Sugar beet pulp (SBP) and molasses, as an agro industrial waste material, are produced in large amounts annually. Thus, a major challenge nowadays is to develop procedures that could increase the value of the generated waste. In this study, SBP as a support for cell immobilization and molasses as a source of nutrients were used for a dextransucrase (DS) production by Leuconostoc mesenteroides T3. The influence of SBP in native form (SBP-N) and after treatment with NaOH (SBP-NaOH) on DS production was investigated. The optimal medium composition for the maximum DS production was determined by varying the concentration of molasses, SBP, and sucrose. The maximum DS yield of 2.02 U/ml was obtained in the medium with 2.5 % of molasses, 2.5 % SBP-NaOH, and 4 % of sucrose concentration. Scanning electron microscopy (SEM) showed immobilization of Lc. mesenteroides T3 cells onto SBP-NaOH. According to the obtained results, the production of DS on molasses could be improved by using NaOH-treated SBP as a carrier for whole-cell immobilization. Our study reveals the basis for the development of process for DS production with additional reduction of expenses by using waste materials for obtaining the valuable biotechnological product.
Department of Biochemical Engineering and Biotechnology, Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, Belgrade, Serbia. mmiljkovic@tmf.bg.ac.rs.
Miona G
MG
Miljković
Department of Biochemical Engineering and Biotechnology, Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, Belgrade, Serbia.
Slađana Z
SZ
Davidović
Department of Biochemical Engineering and Biotechnology, Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, Belgrade, Serbia.
Milica B
MB
Carević
Department of Inorganic Chemical Technology, Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, Belgrade, Serbia.
Đorđe N
ĐN
Veljović
Department of Biochemical Engineering and Biotechnology, Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, Belgrade, Serbia.
Dragana D
DD
Mladenović
Department of Biochemical Engineering and Biotechnology, Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, Belgrade, Serbia.
Mirjana D
MD
Rajilić-Stojanović
Department of Biochemical Engineering and Biotechnology, Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, Belgrade, Serbia.
Suzana I
SI
Dimitrijević-Branković
D061249:Batch Cell Culture Techniques; D027461:Beta vulgaris / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D018914:Cells, Immobilized / Q000378:metabolism; D005285:Fermentation / Q000187:drug effects; D005964:Glucosyltransferases / Q000096:biosynthesis*; D000070007:Leuconostoc mesenteroides / Q000187:drug effects / Q000378:metabolism*; D008965:Molasses / Q000032:analysis*; D013395:Sucrose / Q000494:pharmacology
D013395:Sucrose; D005964:Glucosyltransferases; C020025:dextransucrase
Dextransucrase; Immobilization; Leuconostoc mesenteroides T3; Molasses; Sugar beep pulp