The Association of High Risk Human Papillomaviruses in Patients With Cervical Cancer: An Evidence Based Study on Patients With Squamous Cell Dysplasia or Carcinoma for Evaluation of 23 Human Papilloma Virus Genotypes.
BACKGROUND
Cervical cancer is one of the leading causes of cancer-related death in females. Human papilloma virus (HPV) is the major risk factor of cervical cancer.


OBJECTIVE
The aim of the current study was to explore the frequency and role of 23 different HPVs in patients with cervical cancer.


METHODS
Overall, 117 formalin-fix and paraffin-embedded (FFPE) tissues from cervical cancer patients with squamous cell carcinoma (SCC) or dysplasia were collected from Mirza-Kochakkhan-Jangali hospital, Tehran, Iran during year 2013, to investigate the presence of HPV- HPV- 67, 68, 6, 11, 13, 16, 17, 30, 69, 39, 40, 42, 64, 66 and 51 to 59 genotypes.


RESULTS
The Pap smear report illustrated the presence of malignancy in 71 cases, while 11 cases had no evidence of malignancy. Among the patients, 26 cases had sexually transmitted disease with relative frequency of 0.58. Infection with papilloma virus was observed in 83.6% of SCC patients and 45% of the dysplasia group. The most prevalent HPV genotypes were 18 with 31.62% and 16 with 27.35% of cases. Moreover the relative frequencies of HPV-33, -6, -58, -52, -35 and -51, genotypes were 15.38, 7.69, 5.98, 5.12 and 3.41%, respectively. Among the different genotypes of HPV, 31 had the lowest and 16 had the highest relative frequency.


CONCLUSIONS
Our findings demonstrate that HPV-16 and -18 have a higher prevalence in our population than 31 and 51. Further investigations are required to evaluate the role of these genotypes in a larger multicenter setting for establishing their values for early detection of patients, which is useful for screening and vaccination programs of cancerous and precancerous lesions of cervical cancer.
Microbiology Department, Kashan University of Medical Sciences, Kashan, IR Iran; Autoimmune Diseases Research Center, School of Medicine, Kashan University of Medical Sciences, Kashan, IR Iran.
Ahmad
A
Piroozmand
Central Lab, Isfahan University of Medical Sciences, Isfahan, IR Iran.
Seyed Mostafa
SM
Mostafavi Zadeh
Islamic Azad University, Tehran Medical Sciences Branch, Tehran, IR Iran.
Azita
A
Madani
Department of Biology, Payame Noor University of Isfahan, Isfahan, IR Iran.
Reza
R
Soleimani
Student Research Committee, Department of Medical Biotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IR Iran; Microbiology Department, Deputy of Food and Drug, Isfahan University of Medical Sciences, Isfahan, IR Iran.
Reza
R
Nedaeinia
Microbiology Department, Faculty of Medicine, Shahed University of Medical Sciences, Tehran, IR Iran.
Mohammad
M
Niakan
Molecular Medicine Group, Department of Modern Sciences and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IR Iran.
Amir
A
Avan
Neurosciences Research Center, Alzahra Hospital, Isfahan University of Medical Sciences, Isfahan, IR Iran.
Mostafa
M
Manian
Student Research Committee, Shahed University of Medical Sciences, Tehran, IR Iran.
Mohammad
M
Moradi
Department of Gynecology and Obstetrics, Mirza Khoochak Khan Hospital, Tehran University of Medical Sciences, Tehran, IR Iran.
Zahra
Z
Eftekhar
Cervical Cancer; HPV-16; HPV-18; Papilloma Virus; Polymerase Chain Reaction