Mold Pectinase Modified with Dialdehyde Derivatives of Dextran and Cellulose.
Chemical modification of mold pectinase with dextran- and cellulose-dialdehydes was examined to improve the enzyme characteristics. The modified pectinase with dextran-dialdehyde retained about 50% of the original activity, and more than 80% of the total amino groups were modified. HPLC gel filtration analysis showed an increase in molecular weight of the reaction product. Reaction with cellulose-dialdehyde provided an immobilized form of pectinase. The immobilized pectinase was resistant to both acidic and alkaline pHs, and also acquired heat stability at 60°C. The optimum pH of the modified enzyme shifted from pH 4.5 to 5.0-5.5, and this enzyme had higher activity at neutral pH regions than the native enzyme. A rather low recovery of immobilized enzyme (14.5%) should be improved by the combination with various methods hitherto established.
a National Food Research Institute , Tsukuba 305 , Japan.
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M
Kobayashi
a National Food Research Institute , Tsukuba 305 , Japan.
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Y
Chiba
a National Food Research Institute , Tsukuba 305 , Japan.
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K
Funane
a National Food Research Institute , Tsukuba 305 , Japan.
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S
Ohya
a National Food Research Institute , Tsukuba 305 , Japan.|b Faculty of Education , Hirosaki University , Hirosaki 036 , Japan.
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Y
Kato
beet pulp; cellulose-dialdehyde; chemical modification; dextran-dialdehyde; pectinase