Silver-Lactoferrin Nanocomplexes as a Potent Antimicrobial Agent.
The process of silver immobilization onto and/or into bovine lactoferrin (LTF), the physicochemical properties of bovine lactoferrin and obtained silver-lactoferrin complexes, as well as antibacterial activity of silver-lactoferrin complexes were investigated in this work. Kinetic study of the silver immobilization into lactoferrin was carried out using batch sorption techniques. Spectrometric (MALDI-TOF/TOF-MS, ICP-MS), spectroscopic (FTIR, SERS), electron microscopic (TEM) and electrophoretic (I-DE) techniques, as well as zeta potential measurements, were applied for characterization of LTF and binding nature of silver in Ag-LTF complexes. On the basis of the results of the kinetics study, it was established that the silver binding to LTF is a heterogeneous process involving two main stages: (i) internal diffusion and sorption onto external surface of lactoferrin globules; and (ii) internal diffusion and binding into lactoferrin globule structure. Spectroscopic techniques combined with TEM analysis confirmed the binding process. Molecular dynamics (MD) analysis was carried out in order to simulate the mechanism of the binding process, and locate potential binding sites, as well as complement the experimental findings. Quantum mechanics (QM) simulations were performed utilizing density functional theory (DFT) in order to support the reduction mechanism of silver ions to elemental silver. Antimicrobial activity of synthesized lactoferrin complexes against selected clinical bacteria was confirmed using flow cytometry and antibiograms.
Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Interdisciplinary Centre for Modern Technologies, Nicolaus Copernicus University , 87-100 Toruń, Poland.
Paweł
P
Pomastowski
Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Interdisciplinary Centre for Modern Technologies, Nicolaus Copernicus University , 87-100 Toruń, Poland.
Myroslav
M
Sprynskyy
Department of Chemical Engineering, Pukyong National University , 365 Sinseon-ro, Nam-gu, 608-739 Busan, Korea.
Petar
P
Žuvela
Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Interdisciplinary Centre for Modern Technologies, Nicolaus Copernicus University , 87-100 Toruń, Poland.
Katarzyna
K
Rafińska
Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Interdisciplinary Centre for Modern Technologies, Nicolaus Copernicus University , 87-100 Toruń, Poland.
Maciej
M
Milanowski
Department of Chemical Engineering, Pukyong National University , 365 Sinseon-ro, Nam-gu, 608-739 Busan, Korea.
J Jay
JJ
Liu
Department of Biomedical Engineering, Pukyong National University , 45 Yongso-ro, Nam-gu, 608-737 Busan, Korea.
Myunggi
M
Yi
Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Interdisciplinary Centre for Modern Technologies, Nicolaus Copernicus University , 87-100 Toruń, Poland.
Bogusław
B
Buszewski
D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry*; D001224:Aspartic Acid / Q000737:chemistry; D001419:Bacteria / Q000187:drug effects; D001665:Binding Sites; D002417:Cattle; D015195:Drug Design; D018698:Glutamic Acid / Q000737:chemistry; D007477:Ions; D007781:Lactoferrin / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D056004:Molecular Dynamics Simulation; D036103:Nanotechnology; D011485:Protein Binding; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D011789:Quantum Theory; D012834:Silver / Q000737:chemistry*; D012984:Software; D013499:Surface Properties
D000890:Anti-Infective Agents; D007477:Ions; D001224:Aspartic Acid; D018698:Glutamic Acid; D012834:Silver; D007781:Lactoferrin