Effects of 3 sequestering agents on milk aflatoxin M1 concentration and the performance and immune status of dairy cows fed diets artificially contaminated with aflatoxin B1.
This study examined whether adding 3 mycotoxin-sequestering agents to diets contaminated with aflatoxin B1 (AFB1) would reduce milk aflatoxin M1 (AFM1) concentration, and improve the performance and alter immune status of dairy cows. Fifteen lactating dairy cows were used in an experiment with an incomplete crossover design including four 28-d periods. Treatments included a control diet (C), a toxin diet (T; 1,725µg of AFB1/head per day; 75µg/kg), and diets containing the toxin and 20g/head per day of a proprietary mixture of Saccharomyces cerevisiae fermentation product containing a low (SEQ1) or high (SEQ2) dose of a chlorophyll-based additive, or a low dose of the chlorophyll-based additive and sodium bentonite clay (SEQ3). Sequestering agents were top-dressed on the total mixed ration (TMR) daily in each period, and AFB1 was dosed orally in gelatin capsules before the TMR was fed on d 21 to 25. Milk was sampled twice daily on d 20 to 28 and plasma was sampled on d 20 and 25. Sequestering agents did not affect milk AFM1 concentration during the toxin-dosing period. However, after AFB1 was withdrawn, the sequestering agents reduced the time required (24 vs. 48h) to reduce the milk AFM1 concentration below the Food and Drug Administration action level of 0.5µg/kg. Feeding T instead of C tended to reduce milk and fat-corrected milk yields, but feeding SEQ1 prevented these effects. Red blood cell count and hemoglobin concentration were reduced by feeding T instead of C, but not by feeding SEQ1, SEQ2, or SEQ3. The mean fluorescence intensity of antibody staining for 2 leukocyte adhesion molecules, L-selectin (CD62L) and β-integrin (CD18), tended to be greatest when SEQ1 and SEQ3 were fed. Plasma acid-soluble protein concentration was decreased by feeding SEQ1, SEQ2, and SEQ3 instead of T. Sequestering agents had no effect on milk AFM1 concentration, but they reduced the time required to reduce milk AFM1 concentration to a safe level after withdrawal of AFB1 from the diet. Only SEQ1 prevented the adverse effects of AFB1 on milk and fat-corrected milk yields.
Department of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville 32611.
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Ogunade
Department of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville 32611.
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Arriola
Department of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville 32611.
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Jiang
Department of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville 32611.
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JP
Driver
Department of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville 32611.
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Staples
Department of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville 32611. Electronic address: adesogan@ufl.edu.
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Adesogan
D016604:Aflatoxin B1 / Q000032:analysis*; D016607:Aflatoxin M1 / Q000032:analysis*; D000821:Animal Feed / Q000032:analysis* / Q000382:microbiology; D000818:Animals; D001546:Bentonite / Q000008:administration & dosage; D002214:Capsules; D002417:Cattle; D002734:Chlorophyll / Q000008:administration & dosage; D018592:Cross-Over Studies; D004032:Diet / Q000662:veterinary*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005285:Fermentation; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D039641:Integrin beta Chains / Q000097:blood; D019041:L-Selectin / Q000097:blood; D007774:Lactation; D008892:Milk / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology; D012441:Saccharomyces cerevisiae; D064449:Sequestering Agents / Q000008:administration & dosage*
D002214:Capsules; D039641:Integrin beta Chains; D064449:Sequestering Agents; D019041:L-Selectin; D001546:Bentonite; D002734:Chlorophyll; D016607:Aflatoxin M1; D016604:Aflatoxin B1
aflatoxin; milk; sequestering agent