High extracellular pressure promotes gastric cancer cell adhesion, invasion, migration and suppresses gastric cancer cell differentiation.
Slightly increased pressure stimulates tumor cell adhesion and proliferation. In the present study, we aimed to evaluate the effects of high pressure on gene expression and the biological behavior of gastric cancer cells. After incubation for 30 min at 37˚C under ambient and increased pressure, one portion of SGC7901 cells was used for cell proliferation and apoptosis assays, cell cycle analysis, adhesion invasion or migration assays. The other portion of cells was harvested for detection of matrix metalloproteinase-2 (MMP-2), inhibitor of DNA binding-1 (ID1), sonic Hedgehog (SHH) and E-cadherin expression by western blotting or RT-PCR. In addition, we investigated the effects of high pressure on SGC7901 cell ultrastructure by transmission electron microscopy. We found that the adhesion fold under increased pressure of 760 and 1,520 mmHg was 2.39±1.05 (P<0.05) and 2.47±0.85 (P<0.01) as compared with the control, respectively. The invasion fold was 3.42±2.06 (P<0.05) and 5.13±2.49 (P<0.01) as compared with the control, respectively. The migration was 1.65±0.20 (P<0.001) and 2.53±0.50 (P<0.001) as compared with the control, respectively. At increased pressure, MMP-2 and ID1 expression increased significantly, while the expression of SHH decreased significantly. However, we did not find significant change in proliferation, apoptosis, cell cycle or ultrastructure of the SGC7901 cells under high pressure. In conclusion, high pressure promoted the adhesion, invasion and migration of SGC7901 cells. Moreover, the present study suggests that the pressure-augmented invasion and migration may be related to the increase in MMP-2 expression. Moreover, high pressure may suppress SGC7901 cell differentiation, which may result from the change in SHH and ID1 expression.
Department of General Surgery, The Second Hospital of Harbin Medical University, Harbin 150001, P.R. China.
Changlei
C
Su
Department of Colorectal Surgery, The Third Affiliated Hospital of Harbin Medical University, Harbin 150040, P.R. China.
Bomiao
B
Zhang
College of Automation, Harbin Engineering University, Harbin 150001, P.R. China.
Wenzhi
W
Liu
Department of Surgical Oncology, The Fourth Hospital of Harbin Medical University, Harbin 150001, P.R. China.
Hongqun
H
Zheng
Department of Surgical Oncology, The Fourth Hospital of Harbin Medical University, Harbin 150001, P.R. China.
Lingyu
L
Sun
Department of Surgical Oncology, The Fourth Hospital of Harbin Medical University, Harbin 150001, P.R. China.
Jinxue
J
Tong
College of Automation, Harbin Engineering University, Harbin 150001, P.R. China.
Tian
T
Wang
Clinical Laboratory, The Fourth Hospital of Harbin Medical University, Harbin 150001, P.R. China.
Xiaofeng
X
Jiang
Clinical Laboratory, The Fourth Hospital of Harbin Medical University, Harbin 150001, P.R. China.
Hongyan
H
Liang
Clinical Laboratory, The Fourth Hospital of Harbin Medical University, Harbin 150001, P.R. China.
Li
L
Xue
Department of Surgical Oncology, The Fourth Hospital of Harbin Medical University, Harbin 150001, P.R. China.
Qifan
Q
Zhang
D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D015820:Cadherins / Q000235:genetics; D002448:Cell Adhesion / Q000235:genetics*; D002453:Cell Cycle / Q000235:genetics / Q000502:physiology; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics / Q000502:physiology*; D049109:Cell Proliferation / Q000235:genetics / Q000502:physiology; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics / Q000502:physiology; D053823:Hedgehog Proteins / Q000235:genetics; D006801:Humans; D051798:Inhibitor of Differentiation Protein 1 / Q000235:genetics; D020778:Matrix Metalloproteinase 2 / Q000235:genetics; D009361:Neoplasm Invasiveness / Q000235:genetics* / Q000473:pathology*; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology*
D015820:Cadherins; D053823:Hedgehog Proteins; C497453:ID1 protein, human; D051798:Inhibitor of Differentiation Protein 1; C509239:SHH protein, human; D020778:Matrix Metalloproteinase 2