[Expression of heat shock protein 27 in cigarette smoke extract-induced injury of human gingival fibroblasts].
OBJECTIVE
This research aimed to observe the expression of heat shock protein 27 (HSP27) in human gingival fibroblasts (HGFs) cell injury induced by different concentrations of cigarette smoke extract (CSE).


METHODS
The third to eighth generations of cultured HGFs were treated with serially diluted CSE of different concentrations (0, 2.5%, 5.0%, 12.5%, 25.0%, 50.0%). Wound-healing assay was performed to determine the migration of HGFs, and Western blot was used to determine the expression of HSP27.


RESULTS
The migration capability of HGFs weakened with the increase of CSE concentration. HSP27 expression was negative in normal HGFs but positive in CSE-intervened HGFs in a concentration-dependent manner.


CONCLUSIONS
HSP27 concentration increased in the CSE-induced injury of HGFs. This finding suggests that HSP27 plays an important role in CSE-induced epithelial injury.
Zhou
Z
Qin
Sun
S
Ruinan
Zhu
Z
Zhenkun
D002478:Cells, Cultured; D005347:Fibroblasts; D005881:Gingiva; D055551:HSP27 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D006801:Humans; D018832:Molecular Chaperones; D012906:Smoke; D014026:Nicotiana; D014945:Wound Healing
D055551:HSP27 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D012906:Smoke