A Hydroalcoholic Extract from Paullinia pinnata L. Roots Exerts Anthelmintic Activity against Free-Living and Parasitic Nematodes.
Paullinia pinnata is a medicinal plant traditionally used in West Africa against a wide range of diseases including soil-transmitted helminthiases. In this study, a hydroethanolic root extract was investigated for its phytochemical composition and in vitro activity against the free-living nematode Caenorhabditis elegans as well as the larval stages of the parasitic helminths Ancylostoma caninum, Haemonchus contortus, Toxocara cati, and Trichuris vulpis.LC-MS analysis of the ethanol-water (1 : 1) extract revealed epicatechin and different A-type linked oligomeric and polymeric procyanidins as the predominant compounds.Within an in vitro mortality assay, the extract showed a lethal activity against T. cati (LC50 of 112 µg/mL), T. vulpis (LC50 of 17 µg/mL), and C. elegans (LC50 2.5 of mg/mL), but not against A. caninum. Additionally, effects on egg hatching and larval migration of H. contortus were investigated, but no inhibitory activity was observed.Overall, these findings rationalize the traditional use of the root extract from P. pinnata as an anthelmintic remedy and provide insight into the phytochemical composition of the extract.
Institute for Pharmaceutical Biology and Phytochemistry, University of Münster, Münster, Germany.
Verena
V
Spiegler
Department of Molecular Physiology, Institute for Zoophysiology, Münster, Germany.
Eva
E
Liebau
Institute for Pharmaceutical Biology and Phytochemistry, University of Münster, Münster, Germany.
Carolin
C
Peppler
Institute for Parasitology, University of Veterinary Medicine Hannover, Hannover, Germany.
Katharina
K
Raue
Research Institute of Organic Agriculture (FiBL), Frick, Switzerland.
Steffen
S
Werne
Institute for Parasitology, University of Veterinary Medicine Hannover, Hannover, Germany.
Christina
C
Strube
Research Institute of Organic Agriculture (FiBL), Frick, Switzerland.
Felix
F
Heckendorn
Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Christian
C
Agyare
Food Chemistry & Molecular Sensory Science, Technical University of Munich, Freising, Germany.
Timo
T
Stark
Food Chemistry & Molecular Sensory Science, Technical University of Munich, Freising, Germany.
Thomas
T
Hofmann
Institute for Pharmaceutical Biology and Phytochemistry, University of Münster, Münster, Germany.
Andreas
A
Hensel
D000818:Animals; D000871:Anthelmintics / Q000302:isolation & purification* / Q000494:pharmacology; D017173:Caenorhabditis elegans / Q000187:drug effects*; D002415:Cats; D004285:Dogs; D009348:Nematoda / Q000187:drug effects*; D032126:Paullinia / Q000737:chemistry*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry
D000871:Anthelmintics; D010936:Plant Extracts