A quantitative headspace-solid-phase microextraction-gas chromatography-flame ionization detector method to analyze short chain free fatty acids in rat feces.
This study sought to develop and validate a quantitative method to analyze short chain free fatty acids (SCFAs) in rat feces by solid-phase microextraction and gas chromatography (SPME-GC) using the salt mixture ammonium sulfate and sodium dihydrogen phosphate as salting out agent. Conditioning and extraction time, linearity, limits of detection and quantification, repeatability, and recovery were evaluated. The proposed method allows quantification with improved sensitivity as compared with other methods exploiting SPME-GC. The method has been applied to analyze rat fecal samples, quantifying acetic, propionic, isobutyric, butyric, isopentanoic, pentanoic, and hexanoic acids.
Division of Chemistry, School of Science and Technology, University of Camerino, I-62032 Camerino, MC, Italy. Electronic address: dennis.fiorini@unicam.it.
Dennis
D
Fiorini
Division of Chemistry, School of Science and Technology, University of Camerino, I-62032 Camerino, MC, Italy.
Maria Chiara
MC
Boarelli
School of Pharmacy, University of Camerino, I-62032 Camerino, MC, Italy.
Rosita
R
Gabbianelli
Division of Chemistry, School of Science and Technology, University of Camerino, I-62032 Camerino, MC, Italy.
Roberto
R
Ballini
Department of Agricultural, Food, and Environmental Sciences, Università Politecnica delle Marche, I-60131 Ancona, Italy.
Deborah
D
Pacetti
D000818:Animals; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation* / Q000379:methods*; D002849:Chromatography, Gas; D005230:Fatty Acids, Nonesterified / Q000032:analysis*; D005243:Feces / Q000737:chemistry*; D005410:Flame Ionization; D057230:Limit of Detection; D051381:Rats; D015203:Reproducibility of Results; D052617:Solid Phase Microextraction
D005230:Fatty Acids, Nonesterified
Rat feces; Short chain free fatty acids; Solid-phase microextraction