Comparison of Enzyme Immunoassays for Detection of Antibodies to Hepatitis D Virus in Serum.
Serology remains critical for diagnosing hepatitis D virus (HDV) infection, which affects 15 to 20 million people worldwide, but the literature on characterizing commercial enzyme immunoassays (EIAs) dates back to 15 years ago. We evaluated 2 commercial EIAs currently available for detecting anti-HDV antibodies. The DiaSorin assay demonstrated 100% sensitivity and specificity. Using a modified cutoff value, the Cusabio assay demonstrated a sensitivity of 81.3% and specificity of 90.9%. Our data show that recently developed EIAs are reliable for anti-HDV antibody detection.
Department of Laboratory Medicine, University of Washington, Seattle, Washington, USA.
Siu-Kei
SK
Chow
Department of Laboratory Medicine, University of Washington, Seattle, Washington, USA.
Ederlyn E
EE
Atienza
Department of Laboratory Medicine, University of Washington, Seattle, Washington, USA.
Linda
L
Cook
Focus Diagnostics Reference Laboratory, San Juan Capistrano, California, USA.
Harry
H
Prince
Department of Pathology, University of Utah, Salt Lake City, Utah, USA.
Patricia
P
Slev
Focus Diagnostics Reference Laboratory, San Juan Capistrano, California, USA.
Mary
M
Lapé-Nixon
Department of Laboratory Medicine, University of Washington, Seattle, Washington, USA The Vaccine and Infectious Disease Institute, Fred Hutchinson Cancer Research Center, Seattle, Washington, USA kjerome@fhcrc.org.
Keith R
KR
Jerome
D006508:Hepatitis Antibodies / Q000097:blood*; D003699:Hepatitis D / Q000175:diagnosis*; D003698:Hepatitis Delta Virus / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D044967:Serum / Q000276:immunology
D006508:Hepatitis Antibodies