Nucleopolyhedroviruses (NPV) induce the expression of small heat shock protein 25.4 in Antheraea pernyi.
Nucleopolyhedroviruses (NPVs) is one group of Baculoviruses. The infection of NPV in silkworm is often lethal. To investigate the effective measures to stop the infection of NPV, we cloned cDNA encoding small heat shock protein 25.4 in Antheraea pernyi (Ap-HSP25.4). The translated amino acid sequence consisted of 223 residues with a calculated molecular mass of 25.4kDa and an isoelectronic point (pI) of 4.93. Quantitative real-time PCR was used to investigate the expression patterns and distribution profiles of Ap-sHSP25.4 before and after challenged with NPV. We found that the inhibitors of eicosanoid synthesis could suppress the transcription of Ap-sHSP25.4 in the fat body in a dose dependent manner. And arachidonic acid induced the expression of Ap-sHSP25.4. Thus, we concluded that sHSPs may be promising candidates to boost insect immunity in practice.
College of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, 621010 Sichuan, PR China; College of Life Sciences, Anhui Agricultural University, Hefei, 230036 Anhui, PR China.
Congfen
C
Zhang
College of Life Sciences, Anhui Agricultural University, Hefei, 230036 Anhui, PR China.
Baojian
B
Zhu
College of Life Sciences, Anhui Agricultural University, Hefei, 230036 Anhui, PR China.
Li Shang
LS
Dai
College of Life Sciences, Anhui Agricultural University, Hefei, 230036 Anhui, PR China. Electronic address: cyschx@163.com.
Chaoliang
C
Liu
College of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, 621010 Sichuan, PR China. Electronic address: lxg@swust.edu.cn.
Xuegang
X
Luo
D000818:Animals; D016718:Arachidonic Acid / Q000096:biosynthesis; D003001:Cloning, Molecular; D015777:Eicosanoids / Q000378:metabolism; D019143:Evolution, Molecular; D017344:Genes, Insect; D050888:Heat-Shock Proteins, Small / Q000096:biosynthesis* / Q000235:genetics; D007814:Larva; D009036:Moths / Q000235:genetics / Q000276:immunology / Q000821:virology*; D017924:Nucleopolyhedroviruses / Q000502:physiology*; D013312:Stress, Physiological
D015777:Eicosanoids; D050888:Heat-Shock Proteins, Small; D016718:Arachidonic Acid
Eicosanoids; Heat shock protein 25.4; Nucleopolyhedroviruses; Transcript