Isolation and Characterization of a Novel Tembusu Virus Circulating in Muscovy Ducks in South China.
Duck Tembusu virus (DTMUV) is an infectious pathogen that can cause epidemics in egg-laying ducks. Here, we isolated and characterized a DTMUV, designated GDLH01, thought to be responsible for the noticeable egg drop in Muscovy duck flocks in South China since 2011. The genome sequence of GDLH01 shared 97-99% homology with other avian-origin Tembusu viruses, and 99.5% homology with the mosquito-borne strain SDMS recently reported in China. Phylogenetic analysis based on the nucleotide sequence of the entire open reading frame confirmed that the isolate was of avian origin and closely related to a mosquito-borne strain. Our findings characterize a novel Tembusu virus circulating in Muscovy ducks in South China and emphasize the importance of reinforcing biosecurity measures and developing vaccines to prevent the spread of this viral pathogen.
College of Animal Science, South China Agricultural University, Guangzhou, China.|Guangdong Wen's Foodstuff Group Co. Ltd, Guangdong Enterprise Key Laboratory for Animal Health and Environmental Control, Yunfu, China.
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Z
Yan
College of Animal Science, South China Agricultural University, Guangzhou, China.
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H
Shen
Guangdong Wen's Foodstuff Group Co. Ltd, Guangdong Enterprise Key Laboratory for Animal Health and Environmental Control, Yunfu, China.
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Z
Wang
College of Animal Science, South China Agricultural University, Guangzhou, China.
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W
Lin
College of Animal Science, South China Agricultural University, Guangzhou, China.
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Q
Xie
College of Animal Science, South China Agricultural University, Guangzhou, China.
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Y
Bi
College of Animal Science, South China Agricultural University, Guangzhou, China.
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F
Chen
D000818:Animals; D002681:China / Q000453:epidemiology; D004372:Ducks / Q000821:virology*; D005260:Female; D005416:Flavivirus / Q000235:genetics / Q000302:isolation & purification*; D018177:Flavivirus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D016679:Genome, Viral / Q000235:genetics*; D016366:Open Reading Frames; D010063:Ovum; D010802:Phylogeny; D011201:Poultry Diseases / Q000453:epidemiology / Q000821:virology*; D017422:Sequence Analysis, DNA / Q000662:veterinary
Muscovy duck; Tembusu virus; isolation; phylogenetic analysis