Mimicking Form and Function of Native Small Diameter Vascular Conduits Using Mulberry and Non-mulberry Patterned Silk Films.
Autologous graft replacement as a strategy to treat diseased peripheral small diameter (≤6 mm) blood vessel is often challenged by prior vein harvesting. To address this issue, we fabricated native-tissue mimicking multilayered small diameter vascular graft (SDVG) using mulberry (Bombyx mori) and Indian endemic non-mulberry (Antheraea assama and Philosamia ricini) silk. Patterned silk films were fabricated on microgrooved PDMS mold, casted by soft lithography. The biodegradable patterned film templates with aligned cell sheets were rolled onto an inert mandrel to mimic vascular conduit. The hemocompatible and mechanically strong non-mulberry films with RGD motif supported ∼1.2 folds greater proliferation of vascular cells with aligned anchorage. Elicitation of minimal immune response on subcutaneous implantation of the films in mice was complemented by ∼45% lower TNF α secretion by in vitro macrophage culture post 7 days. Pattern-induced alignment favored the functional contractile phenotype of smooth muscle cells (SMCs), expressing the signature markers-calponin, α-smooth muscle actin (α-SMA), and smooth muscle myosin heavy chain (SM-MHC). Endothelial cells (ECs) exhibited a typical punctuated pattern of von Willebrand factor (vWF). Deposition of collagen and elastin by the SMCs substantiated the aptness of the graft with desired biomechanical attributes. Furthermore, the burst strength of the fabricated conduit was in the range of ∼915-1260 mmHg, a prerequisite to withstand physiological pressure. This novel fabrication approach may eliminate the need of maturation in a pulsatile bioreactor for obtaining functional cellular phenotype. This work is thereby an attestation to the immense prospects of exploring non-mulberry silk for bioengineering a multilayered vascular conduit similar to a native vessel in "form and function", befitting for in vivo transplantation.
Biomaterial and Tissue Engineering Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati , Guwahati-781039, Assam, India.
Prerak
P
Gupta
Biomaterial and Tissue Engineering Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati , Guwahati-781039, Assam, India.
Manishekhar
M
Kumar
Life Science Division, Institute of Advanced Study in Science and Technology (IASST) , Guwahati-781035, Assam, India.
Nandana
N
Bhardwaj
Biomaterial and Tissue Engineering Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati , Guwahati-781039, Assam, India.
Jadi Praveen
JP
Kumar
Biomaterial and Tissue Engineering Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati , Guwahati-781039, Assam, India.
C S
CS
Krishnamurthy
Department of Veterinary Surgery and Radiology, West Bengal University of Animal and Fishery Sciences , Kolkata-700037, West Bengal, India.
Samit Kumar
SK
Nandi
Biomaterial and Tissue Engineering Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati , Guwahati-781039, Assam, India.
Biman B
BB
Mandal
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology / Q000592:standards; D003094:Collagen / Q000378:metabolism; D020007:Implants, Experimental; D051379:Mice; D031625:Morus / Q000378:metabolism; D009036:Moths / Q000737:chemistry; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects*; D047011:Silk / Q000737:chemistry / Q000494:pharmacology*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry / Q000592:standards*
D001672:Biocompatible Materials; D047011:Silk; D003094:Collagen
biomaterials; non-mulberry silk; silk; tissue engineering; vascular graft