Systematic development and optimization of chemically defined medium supporting high cell density growth of Bacillus coagulans.
With determined components and experimental reducibility, the chemically defined medium (CDM) and the minimal chemically defined medium (MCDM) are used in many metabolism and regulation studies. This research aimed to develop the chemically defined medium supporting high cell density growth of Bacillus coagulans, which is a promising producer of lactic acid and other bio-chemicals. In this study, a systematic methodology combining the experimental technique with flux balance analysis (FBA) was proposed to design and simplify a CDM. The single omission technique and single addition technique were employed to determine the essential and stimulatory compounds, before the optimization of their concentrations by the statistical method. In addition, to improve the growth rationally, in silico omission and addition were performed by FBA based on the construction of a medium-size metabolic model of B. coagulans 36D1. Thus, CDMs were developed to obtain considerable biomass production of at least five B. coagulans strains, in which two model strains B. coagulans 36D1 and ATCC 7050 were involved.
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, P.O. box 329, 130 Meilong Road, Shanghai, 200237, China.
Yu
Y
Chen
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, P.O. box 329, 130 Meilong Road, Shanghai, 200237, China.
Fengqing
F
Dong
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, P.O. box 329, 130 Meilong Road, Shanghai, 200237, China. yhwang@ecust.edu.cn.
Yonghong
Y
Wang
D000070000:Bacillus coagulans / Q000254:growth & development*; D003198:Computer Simulation; D003470:Culture Media / Q000737:chemistry*; D064688:Metabolic Flux Analysis; D008954:Models, Biological
D003470:Culture Media
Bacillus coagulans; Chemically defined medium; Flux balance analysis; Lactic acid; Medium optimization