Antioxidant and hepatoprotective activities of intracellular polysaccharide from Pleurotus eryngii SI-04.
In present study, the intracellular polysaccharide (IPS) and its two fractions of IPS-1 and IPS-2 were obtained and purified by DEAE-52 cellulose chromatography from Pleurotus eryngii SI-04 mycelia, and their hepatoprotective effects were also investigated. The results showed that the IPS-2 had superior hepatoprotective effects by increasing the serum enzyme activities and bilirubin (BIL) levels, decreasing the serum albumin (ALB) and triglyceride (TG) levels, improving the hepatic antioxidant status, and ameliorating the hepatic structure damage. Furthermore, the monosaccharide composition and main bond types were also analyzed. These conclusions demonstrated that the both IPS and its fractions might be suitable for functional foods and natural drugs in preventing the acute liver damage.
College of Life Science, Shandong Agricultural University, Taian 271018, China.
Chen
C
Zhang
College of Life Science, Shandong Agricultural University, Taian 271018, China.
Shangshang
S
Li
College of Life Science, Shandong Agricultural University, Taian 271018, China.
Jianjun
J
Zhang
College of Forestry, Shandong Agricultural University, Taian 271018, China.
Chunlong
C
Hu
Shandong Academy of Agricultural Science, Ji'nan 250100, China.
Gen
G
Che
Quality and safety Monitoring Center of Animal Products, Ji'nan 250002, China.
Meng
M
Zhou
College of Life Science, Shandong Agricultural University, Taian 271018, China. Electronic address: jiale0525@163.com.
Le
L
Jia
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D002251:Carbon Tetrachloride; D002849:Chromatography, Gas; D022781:Hepatocytes / Q000187:drug effects / Q000473:pathology; D042541:Intracellular Space / Q000737:chemistry*; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D008297:Male; D051379:Mice; D009005:Monosaccharides / Q000032:analysis; D009929:Organ Size / Q000187:drug effects; D020076:Pleurotus / Q000737:chemistry*; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D044967:Serum / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared
D000975:Antioxidants; D009005:Monosaccharides; D011134:Polysaccharides; D020011:Protective Agents; D002251:Carbon Tetrachloride
Antioxidant and hepatoprotective activity; Intracellular polysaccharide; Pleurotus eryngii SI-04