Proteomics data in support of the quantification of the changes of bovine milk proteins during mammary gland involution.
Here we provide data from three proteomics techniques; two-dimensional electrophoresis (2-DE) followed by identification of selected spots using PSD MALDI-TOF MS/MS, one-dimensional gel electrophoresis followed by LC-MS/MS analysis of gel slices (GeLC) and dimethyl isotopic labelling of tryptic peptides followed by Orbitrap MS/MS (DML), to quantify the changes in the repertoire of bovine milk proteins that occurs after drying off. We analysed skim milk and whey sampled at day 0 and either day 3 or day 8 after drying off. These analyses identified 45 spots by MALDI-TOF, 51 proteins by GeLC and 161 proteins by DML, for which the detailed data work-up is presented as three Excel files. The data supplied in this article supports the accompanying publication "Changes in the repertoire of bovine milk proteins during mammary involution" (Boggs et al., 2015) [1]. Data are available via ProteomeXchange with identifiers ProteomeXchange: PXD003110 and ProteomeXchange: PXD003011.
Dairy Foods, AgResearch, Ruakura Research Centre, Private Bag 3123, Hamilton 3240, New Zealand.
Irina
I
Boggs
CSIRO, Agriculture Flagship, Chiswick, Armidale, NSW 2350, Australia.
Brad
B
Hine
Dairy Foods, AgResearch, Ruakura Research Centre, Private Bag 3123, Hamilton 3240, New Zealand.
Grant
G
Smolenksi
Wageningen University, Dairy Science and Technology, FQD Group, PO Box 17, 9, 6700AA Wageningen, The Netherlands.
Kasper
K
Hettinga
Wageningen University, Dairy Science and Technology, FQD Group, PO Box 17, 9, 6700AA Wageningen, The Netherlands.
Lina
L
Zhang
Dairy Foods, AgResearch, Ruakura Research Centre, Private Bag 3123, Hamilton 3240, New Zealand.
Thomas T
TT
Wheeler
Dairy; Drying off; Host defence; Proteomics