Chromatographic resolution of angiotensin II receptor antagonists (sartans).
First time a simple, sensitive and unified quantification method has been developed to analyze the complete class of angiotensin II receptor antagonists which are used in the treatment of hypertension either alone or in combination with some other drugs. The most important advantage of developed method was that the eight separate drugs can be determined on a single chromatographic system without modifications in detection wavelength and mobile phase. The drugs were separated on a Purospher Star 4.6mm×25cm, 5μm, C18 column maintained at 40°C with 1mLmin(-1) flow rate using ultra violet detection at 254nm. Good separation (Rs>2.0) was achieved in a short analysis allowing simultaneous determination of all eight sartans. The effect of variation in flow rate, detection wavelength and column oven temperature was also studied. The proposed method was statistically validated in terms of precision, accuracy, linearity, specificity and robustness. The newly developed method proved to be specific, robust and accurate for the quantification of eight sartans in commercial pharmaceutical formulations.
Department of Chemistry, Government College University, Lahore, Pakistan. Electronic address: saqlain_highnoon@yahoo.com.
Muhammad Saqlain
MS
Tahir
Department of Chemistry, Government College University, Lahore, Pakistan.
Ahmad
A
Adnan
Biotechnology and Food Research Centre, PCSIR, Lahore, Pakistan.
Quratulain
Q
Syed
D047228:Angiotensin II Type 1 Receptor Blockers / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D057230:Limit of Detection; D016014:Linear Models; D015203:Reproducibility of Results; D013607:Tablets
D047228:Angiotensin II Type 1 Receptor Blockers; D013607:Tablets
Drug analogues; HPLC; Sartans; Unified quantification; Validation