Flavisolibacter tropicus sp. nov., isolated from tropical soil.
A Gram-stain-negative, non-motile, deep yellow, rod-shaped bacterium, designated strain LCS9T, was isolated from a soil sample at the tropical zone within the Ecorium of the National Institute of Ecology in Seocheon, central-western Korea. 16S rRNA gene sequence analysis showed that strain LCS9T clustered with members of the genus Flavisolibacter of the family Chitinophagaceae, phylum Bacteroidetes. Sequence similarities between strain LCS9T and the type strains of the genus Flavisolibacter ranged from 94.6 to 94.9 %. Strain LCS9T grew at 10-37 °C (optimum, 25 °C) and at pH 6.0-10.0 (optimum, pH 7); was positive for catalase and oxidase; and negative for nitrate reduction and production of indole. Cells showed pigment absorbance peaks at 451 and 479 nm, and had 0.03 % survival following exposure to 3 kGy gamma radiation. Strain LCS9T had the following chemotaxonomic characteristics: the major quinone was menaquinone-7 (MK-7); the major fatty acids were iso-C15 : 0 and iso-C17 : 0 3-OH; polar lipids included phosphoatidylethanolamine, an unidentified aminophospholipid, unidentified aminolipidsand unidentified lipids. The DNA G+C content was 39.4 mol%. Based on polyphasic analysis, the type strain LCS9T (=KCTC 42070T=JCM 19972T) represents a novel species for which the name Flavisolibacter tropicus sp. nov. is proposed. Radiation resistance in the genus Flavisolibacter has not been reported to date, and so this is the first report of low-level radiation resistance of a member of the genus.
College of Agricultural and Life Sciences, Kyungpook National University, Daegu 41566, Republic of Korea.
Jae-Jin
JJ
Lee
Microorganism Resources Division, National Institute of Biological Resources, Incheon 404-170, Republic of Korea.
Myung-Suk
MS
Kang
Department of Nature Survey, National Institute of Ecology, Seocheon 33657, Republic of Korea.
Gyung Soon
GS
Kim
Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul 139-774, Republic of Korea.
Chang Soek
CS
Lee
Radiation Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup 580-185, Republic of Korea.
Sangyong
S
Lim
Honor's Class, Seoul Women's University, Seoul 139-774, Republic of Korea.
Jidam
J
Lee
Honor's Class, Seoul Women's University, Seoul 139-774, Republic of Korea.
Si Hyeon
SH
Roh
Honor's Class, Seoul Women's University, Seoul 139-774, Republic of Korea.
Hyerin
H
Kang
Honor's Class, Seoul Women's University, Seoul 139-774, Republic of Korea.
Jung Min
JM
Ha
Honor's Class, Seoul Women's University, Seoul 139-774, Republic of Korea.
Sojung
S
Bae
College of Agricultural and Life Sciences, Kyungpook National University, Daegu 41566, Republic of Korea.
Hee-Young
HY
Jung
Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul 139-774, Republic of Korea.
Myung Kyum
MK
Kim
D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D010858:Pigmentation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014329:Tropical Climate; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7