Profiling and analysis of multiple compounds in rhubarb decoction after processing by wine steaming using UHPLC-Q-TOF-MS coupled with multiple statistical strategies.
Rhubarb is one of the most popular traditional Chinese medicines and has been used for thousands of years in many Asian countries. Prepared rhubarb is obtained by steaming raw rhubarb with glutinous rice wine until it turned black in appearance both inside and outside. After processing, the therapeutic effects of prepared rhubarb change a lot. To find out the exact compound changes of the chemical profile in a decoction of rhubarb after processing and to clarify the material basis of the changed therapeutic effects, an ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry method coupled with automated data analysis software and statistical strategy was developed. As a result, 63 peaks in raw rhubarb and 54 peaks in prepared rhubarb were detected, and a total of 45 chemical compounds were identified. The analysis data were subjected to a principle component analysis and a t-test. Based on the results, 16 peaks were found to be the main contributors to the significant difference (p < 0.05) between raw and prepared rhubarb. Compared with raw rhubarb, the content of 15 components in prepared rhubarb was lower, while only rhein (1,8-dihydroxy-3-carboxy anthraquinone) showed a higher intensity.
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, PR China.|Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, PR China.
Tingting
T
Zhu
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, PR China.|Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, PR China.|Nanjing Haichang Chinese Medicine Group Corporation, Nanjing, PR China.
Xiao
X
Liu
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, PR China.|Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, PR China.|Nanjing Haichang Chinese Medicine Group Corporation, Nanjing, PR China.
Xiaoli
X
Wang
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, PR China.|School of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, PR China.
Gang
G
Cao
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, PR China.|Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, PR China.|Nanjing Haichang Chinese Medicine Group Corporation, Nanjing, PR China.
Kunming
K
Qin
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, PR China.|Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, PR China.
Ke
K
Pei
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, PR China.|Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, PR China.
Hui
H
Zhu
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, PR China.|Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, PR China.|Nanjing Haichang Chinese Medicine Group Corporation, Nanjing, PR China.
Hao
H
Cai
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, PR China.|Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, PR China.
Minjie
M
Niu
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, PR China.|Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing, PR China.|Nanjing Haichang Chinese Medicine Group Corporation, Nanjing, PR China.
Baochang
B
Cai
D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry; D008516:Medicine, Chinese Traditional; D025341:Principal Component Analysis; D012250:Rheum / Q000737:chemistry*; D013227:Steam / Q000032:analysis; D014920:Wine / Q000032:analysis*
D013227:Steam
Principal component analysis; Rhubarb; Traditional Chinese medicine; t-test