Identification of a novel subgroup 16SrII-U phytoplasma associated with papaya little leaf disease.
Papaya is an important fruit crop cultivated in tropical and subtropical regions. Papaya little leaf (PLL) disease was observed in China. The phytoplasma 16S rRNA gene was detected from symptomatic papaya trees via PCR using phytoplasma universal primers P1/P7 followed by R16F2n/R16R2. No amplification products were obtained from templates of asymptomatic papaya trees. These results indicated a direct association between phytoplasma infection and PLL disease. Comparative and phylogenetic analyses of 16S rRNA gene sequences indicated that the papaya-infecting phytoplasmas under study belonged to the peanut witches' broom phytoplasma group (16SrII). Genotyping through use of computer-simulated RFLP analysis of 16S rRNA genes and coefficients of RFLP pattern similarities (0.97) reveal that the PLL phytoplasma was placed in a new subgroup. In this article, we describe the molecular characterization of a new phytoplasma associated with PLL disease and propose that the PLL phytoplasma be considered as a novel subgroup, 16SrII-U.
Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences; Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture; Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Haikou 571101, PR China.
Yi
Y
Yang
Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences; Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture; Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Haikou 571101, PR China.
Lei
L
Jiang
Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences; Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture; Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Haikou 571101, PR China.
Haiyan
H
Che
Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences; Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture; Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Haikou 571101, PR China.
Xueren
X
Cao
Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences; Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture; Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Haikou 571101, PR China.
Daquan
D
Luo
D015373:Bacterial Typing Techniques; D001482:Base Composition; D029441:Carica / Q000382:microbiology*; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005838:Genotype; D010802:Phylogeny; D045582:Phytoplasma / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010935:Plant Diseases / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S