Gastrointestinal transport of calcium and glucose in lactating ewes.
During lactation, mineral and nutrient requirements increase dramatically, particularly those for Ca and glucose. In contrast to monogastric species, in ruminants, it is rather unclear to which extend this physiological change due to increased demand for milk production is accompanied by functional adaptations of the gastrointestinal tract (GIT). Therefore, we investigated potential modulations of Ca and glucose transport mechanisms in the GIT of lactating and dried-off sheep. Ussing-chamber technique was applied to determine the ruminal and jejunal Ca flux rates. In the jejunum, electrophysiological properties in response to glucose were recorded. Jejunal brush-border membrane vesicles (BBMV) served to characterize glucose uptake via sodium-linked glucose transporter 1 (SGLT1), and RNA and protein expression levels of Ca and glucose transporting systems were determined. Ruminal Ca flux rate data showed a trend for higher absorption in lactating sheep. In the jejunum, small Ca absorption could only be observed in lactating ewes. From the results, it may be assumed that lactating ewes compensate for the Ca loss by increasing bone mobilization rather than by increasing supply through absorption from the GIT Presence of SGLT1 in the jejunum of both groups was shown by RNA and protein identification, but glucose uptake into BBMV could only be detected in lactating sheep. This, however, could not be attributed to electrogenic glucose absorption in lactating sheep under Ussing-chamber conditions, providing evidence that changes in jejunal glucose uptake may include additional factors, that is, posttranslational modifications such as phosphorylation.
Department of Physiology, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany stefanie.klinger@tiho-hannover.de.
Stefanie
S
Klinger
Department of Physiology, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany.
Bernd
B
Schröder
Department of Physiology, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany.
Anja
A
Gemmer
Department of Physiology, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany.
Julia
J
Reimers
Department of Physiology, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany.
Gerhard
G
Breves
Department of Physiology, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany.
Jens
J
Herrmann
Department of Physiology, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany.
Mirja R
MR
Wilkens
D000818:Animals; D001692:Biological Transport / Q000502:physiology; D002118:Calcium / Q000378:metabolism*; D005260:Female; D005947:Glucose / Q000378:metabolism*; D007408:Intestinal Absorption / Q000502:physiology; D007583:Jejunum / Q000378:metabolism*; D007774:Lactation / Q000378:metabolism*; D008871:Microvilli / Q000378:metabolism; D012756:Sheep; D051269:Sodium-Glucose Transporter 1 / Q000235:genetics / Q000378:metabolism*
D051269:Sodium-Glucose Transporter 1; D005947:Glucose; D002118:Calcium
Ca absorption; calcium; gastrointestinal tract; glucose; lactation; sheep