Development and applications of molecularly imprinted polymers based on hydrophobic CdSe/ZnS quantum dots for optosensing of N(ε)-carboxymethyllysine in foods.
A novel molecularly imprinted polymer (MIP) based on hydrophobic CdSe/ZnS quantum dots (HBQDs) was synthesized via an approach that combines MIPs for the selectivity and sensitivity with semiconductor HBQDs as optosensing material (HBQDs@MIP). Here, using a one-pot water-in-oil reverse microemulsion polymerization, the HBQDs@MIP was used as a molecular recognition element to construct an N(ε)-carboxymethyllysine (CML) optosensor. The HBQDs were coated with silica to endow the nanoparticles with high dispersibility and broad compatibility with hydrophilic analytes. Under optima conditions, the relative fluorescence intensity of the HBQDs@MIP decreased linearly with increasing CML concentration in the range 5-400μgL(-1). The proposed method enables, detection procedures to be completed within 90min, with good recoveries, ranging from 85% to 106%. It can be applied to the rapid and cost-effective monitoring of CML in food samples.
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University (BTBU), 11 Fucheng Road, Beijing 100048, China.
Huilin
H
Liu
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University (BTBU), 11 Fucheng Road, Beijing 100048, China.
Dan
D
Wu
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University (BTBU), 11 Fucheng Road, Beijing 100048, China.
Kaiwen
K
Zhou
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University (BTBU), 11 Fucheng Road, Beijing 100048, China. Electronic address: wangjing@th.btbu.edu.cn.
Jing
J
Wang
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University (BTBU), 11 Fucheng Road, Beijing 100048, China.
Baoguo
B
Sun
D005506:Food Contamination / Q000032:analysis; D057927:Hydrophobic and Hydrophilic Interactions; D054802:Molecular Imprinting / Q000379:methods*; D011108:Polymers / Q000737:chemistry*; D045663:Quantum Dots / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods
D011108:Polymers
Hydrophobic quantum dots; Molecularly imprinted polymer; N(ε)-Carboxymethyllysine; Optosensing material; Reverse microemulsion