Molecular and Morpho-Agronomical Characterization of Root Architecture at Seedling and Reproductive Stages for Drought Tolerance in Wheat.
Water availability is a major limiting factor for wheat (Triticum aestivum L.) production in rain-fed agricultural systems worldwide. Root architecture is important for water and nutrition acquisition for all crops, including wheat. A set of 158 diverse wheat genotypes of Australian (72) and Indian (86) origin were studied for morpho-agronomical traits in field under irrigated and drought stress conditions during 2010-11 and 2011-12.Out of these 31 Indian wheat genotypes comprising 28 hexaploid (Triticum aestivum L.) and 3 tetraploid (T. durum) were characterized for root traits at reproductive stage in polyvinyl chloride (PVC) pipes. Roots of drought tolerant genotypes grew upto137cm (C306) as compared to sensitive one of 63cm with a mean value of 94.8cm. Root architecture traits of four drought tolerant (C306, HW2004, HD2888 and NI5439) and drought sensitive (HD2877, HD2012, HD2851 and MACS2496) genotypes were also observed at 6 and 9 days old seedling stage. The genotypes did not show any significant variation for root traits except for longer coleoptiles and shoot and higher absorptive surface area in drought tolerant genotypes. The visible evaluation of root images using WinRhizo Tron root scanner of drought tolerant genotype HW2004 indicated compact root system with longer depth while drought sensitive genotype HD2877 exhibited higher horizontal root spread and less depth at reproductive stage. Thirty SSR markers were used to study genetic variation which ranged from 0.12 to 0.77 with an average value of 0.57. The genotypes were categorized into three subgroups as highly tolerant, sensitive, moderately sensitive and tolerant as intermediate group based on UPGMA cluster, STRUCTURE and principal coordinate analyses. The genotypic clustering was positively correlated to grouping based on root and morpho-agronomical traits. The genetic variability identified in current study demonstrated these traits can be used to improve drought tolerance and association mapping.
National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, Pusa Campus, New Delhi, 110012, India.
Ram Sewak Singh
RS
Tomar
National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, Pusa Campus, New Delhi, 110012, India.
Sushma
S
Tiwari
Division of Genetics, Indian Agricultural Research Institute, Pusa Campus, New Delhi, 110012, India.
Vinod
Division of Genetics, Indian Agricultural Research Institute, Pusa Campus, New Delhi, 110012, India.
Bhojaraja K
BK
Naik
School of life Sciences, Devi Ahilya Vishwa Vidyalaya, Khandwa Road, Indore, 452017, India.
Suresh
S
Chand
Departement de Phytologie, University Laval, Quebec, QC, G1V0A6, Canada.
Rupesh
R
Deshmukh
Division of Genetics, Indian Agricultural Research Institute, Pusa Campus, New Delhi, 110012, India.
Niharika
N
Mallick
Directorate of Wheat Research, Karnal, Haryana, 132001, India.
Sanjay
S
Singh
National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, Pusa Campus, New Delhi, 110012, India.
Nagendra Kumar
NK
Singh
Division of Genetics, Indian Agricultural Research Institute, Pusa Campus, New Delhi, 110012, India.
S M S
SM
Tomar
D000064:Acclimatization / Q000502:physiology*; D001315:Australia; D055864:Droughts; D014644:Genetic Variation / Q000235:genetics; D005838:Genotype; D010641:Phenotype; D018517:Plant Roots / Q000502:physiology*; D011891:Rain; D012098:Reproduction; D036226:Seedlings / Q000502:physiology*; D013312:Stress, Physiological; D014908:Triticum / Q000502:physiology*; D014867:Water
D014867:Water