rs11895168 C allele and the increased risk of breast cancer in Isfahan population.
OBJECTIVE
Some of the single nucleotide polymorphisms in EebB4 gene have been studied to date in order for finding their relevance to the risk of breast cancer. However, no study has been conducted to investigate the importance of rs11895168, a microRNA-related SNP located in ErbB4 3'UTR, in breast tumors. In this paper, we investigated the frequency and association between rs11895168 and breast cancer.


METHODS
The rs11895168 was genotyped in 364 samples collected from 172 breast cancer patients and 192 healthy participants, using Tetra-primer ARMS PCR. The frequency of genotypes was analyzed to find the association between rs11895168 and breast cancer risk and also clinicopathological characteristics of patients.


RESULTS
our in silico studies suggested that different alleles at rs11895168 affect the binding strength of miR-1276, a potential tumor suppressor. Statistical analysis showed a significant association between rs11895168 C allele-harboring genotypes and increased breast cancer risk.


CONCLUSIONS
rs11895168 C allele is strongly and significantly associated with the increased risk of breast cancer and positivity of ER/PR tumor cells.
Biochemistry Department, Payam-e Noor University of Isfahan, Isfahan, Iran.
Zohreh
Z
Salimi
Division of Genetics, Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran.
Samira
S
Sadeghi
Division of Genetics, Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran.
Hossein
H
Tabatabaeian
Division of Cellular and Molecular Biology, Department of Biology, Faculty of Sciences, University of Isfahan, PO Box: 81746-73441, Isfahan, Iran; Department of Cellular Biotechnology at Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran. Electronic address: Kamranghaedi@sci.ui.ac.ir.
Kamran
K
Ghaedi
Biochemistry Department, Payam-e Noor University of Isfahan, Isfahan, Iran.
Mohammad
M
Fazilati
D020413:3' Untranslated Regions; D000328:Adult; D000483:Alleles; D001943:Breast Neoplasms / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D005838:Genotype; D006801:Humans; D007492:Iran; D035683:MicroRNAs / Q000378:metabolism; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D066247:Receptor, ErbB-4 / Q000235:genetics*; D012307:Risk Factors
D020413:3' Untranslated Regions; C000614942:MIRN-1276 microRNA, human; D035683:MicroRNAs; C587689:ERBB4 protein, human; D066247:Receptor, ErbB-4
Breast cancer; ErbB4; Tetra-primer ARMS-PCR; microRNA-related single nucleotide polymorphism; rs11895168