Curcumin and Ellagic acid synergistically induce ROS generation, DNA damage, p53 accumulation and apoptosis in HeLa cervical carcinoma cells.
Cervical cancer and precancerous lesions of the cervix continue to be a global health issue, and the medication for the treatment for chronic HPV infection so far has not been effective. Potential anticancer and anti HPV activities of two known phytochemicals, Curcumin and Ellagic acid were evaluated in HeLa cervical cancer cells. Curcumin is a natural compound found in the root of Curcuma longa plant and Ellagic acid a polyphenol found in fruits of strawberries, raspberries and walnuts. The combination of Curcumin and Ellagic acid at various concentrations showed better anticancer properties than either of the drug when used alone as evidenced by MTT assay. Besides this, Curcumin and Ellagic acid also restore p53, induce ROS formation and DNA damage. Mechanistic study further indicated that Curcumin and Ellagic acid show anti-HPV activity as evidenced by decrease in the HPV E6 oncoprotein on HeLa cells.
Department of Biotechnology, Utkal University, Bhubaneswar, Odisha, India.
Devbrat
D
Kumar
Department of Biotechnology, Utkal University, Bhubaneswar, Odisha, India.
Soumya
S
Basu
Department of Zoology, North Orissa University, Baripada, Mayurbhanj, Odisha, India.
Lucy
L
Parija
Department of Biotechnology, Utkal University, Bhubaneswar, Odisha, India.
Deeptimayee
D
Rout
Department of Biotechnology, Utkal University, Bhubaneswar, Odisha, India.
Sanjeet
S
Manna
Department of Biotechnology, Utkal University, Bhubaneswar, Odisha, India.
Jagneshwar
J
Dandapat
Department of Zoology, North Orissa University, Baripada, Mayurbhanj, Odisha, India. Electronic address: prdebata@gmail.com.
Priya Ranjan
PR
Debata
D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D003474:Curcumin / Q000494:pharmacology*; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004610:Ellagic Acid / Q000494:pharmacology*; D005260:Female; D005455:Fluorescent Antibody Technique; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D055550:Protein Stability / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000473:pathology*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism
D050759:Cyclin-Dependent Kinase Inhibitor p21; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D016159:Tumor Suppressor Protein p53; D014764:Viral Proteins; D051028:bcl-2-Associated X Protein; D004610:Ellagic Acid; D003474:Curcumin
Apoptosis; Cervical cancer; Curcumin; Ellagic acid; HeLa cells; p53