Genome-scale CRISPR pooled screens.
Genome editing technologies such as clustered regularly interspaced short palindromic repeats (CRISPR) systems have ushered in a new era of targeted DNA manipulation. The easy programmability of CRISPR using short oligonucleotides enables rapid synthesis of large-scale libraries for functional genetic screens. Here we present fundamental concepts and methods for pooled CRISPR screens and review biological results from recent genome-scale loss-of-function and gain-of-function screens. We also discuss new frontiers in pooled screens, including novel effector domains for functional screens and applications in the noncoding genome.
New York Genome Center, New York, NY 10013, USA; Center for Genomics and Systems Biology, Department of Biology, New York University, New York, NY 10012, USA. Electronic address: nsanjana@nygenome.org.
Neville E
NE
Sanjana
D064113:CRISPR-Cas Systems / Q000235:genetics*; D000072669:Gene Editing / Q000379:methods*; D018390:Gene Targeting / Q000379:methods*; D005818:Genetic Engineering; D015894:Genome, Human; D006801:Humans; D000094704:RNA, Guide, CRISPR-Cas Systems / Q000235:genetics*
D000094704:RNA, Guide, CRISPR-Cas Systems
CRISPR; Cancer; Cas9; Genetic screens; Genome engineering; Noncoding