The Effect of Prednisolone on miR 15a and miR16-1 Expression Levels and Apoptosis in Acute Lymphoblastic Leukemia Cell Line: CCRF-CEM.
BACKGROUND
Glucocorticoids are probably the most important drugs in the treatment of childhood acute lymphoblastic leukemia (ALL). Prednisolone exerts its effect by induce apoptosis in lymphoid lineage cells. Micro RNAs are 18-24 nucleotides RNA implicated in the control of essential biological functions, including apoptosis. In the following study, the effect of prednisolone on the expression of miR 15a & miR16-1 and apoptosis in the CCRF-CEM cell line is investigated.


METHODS
The cell line of CCRF-CEM was cultured in standard conditions. The changes in the miR 15a and miR 16-1 expression levels were determined by Real Time-PCR technique. Also, the apoptosis is evaluated by flow cytometry using Annexin V and PI staining.


RESULTS
This study revealed that, the prednisolone induced apoptosis in a time dependent manner. Prednisolone in concentration of 700 µM was significantly increased the expression of miR 16-1 and miR 15a after 24 h and 48 h treatment (p<0.05).


CONCLUSIONS
prednisolone-induced apoptosis might be mediated by up-regulation of these 2 miRNAs in CCRF-CEM cells.
Department of Basic Sciences, Maragheh University of Medical Sciences, Maragheh, Iran.
A
A
Azimi
Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
M F
MF
Hagh
Department of Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
B
B
Yousefi
Department of Hematology, Faculty of Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
M A
MA
Rahnama
Department of Basic Sciences, Maragheh University of Medical Sciences, Maragheh, Iran.
A
A
Khorrami
Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
M Z
MZ
Heydarabad
Medical Biotechnology Research Center, Guilan University of Medical Sciences, Rasht, Iran.
M
M
Najafpour
Department of Basic Sciences, Maragheh University of Medical Sciences, Maragheh, Iran.
J
J
Hallajzadeh
Department of Basic Sciences, Maragheh University of Medical Sciences, Maragheh, Iran.
A
A
Ghahremani
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000473:pathology*; D011239:Prednisolone / Q000494:pharmacology*; D015854:Up-Regulation
D000970:Antineoplastic Agents; C505361:MIRN15 microRNA, human; C505362:MIRN16 microRNA, human; D035683:MicroRNAs; D011239:Prednisolone