PCI-24781 can improve in vitro and in vivo developmental capacity of pig somatic cell nuclear transfer embryos.
OBJECTIVE
To examine the effect of PCI-24781 (abexinostat) on the blastocyst formation rate in pig somatic cell nuclear transferred (SCNT) embryos and acetylation levels of the histone H3 lysine 9 and histone H4 lysine 12.


RESULTS
Treatment with 0.5 nM PCI-24781 for 6 h significantly improved the development of cloned embryos, in comparison to the control group (25.3 vs. 10.5 %, P < 0.05). Furthermore, PCI-24781 treatment led to elevated acetylation of H3K9 and H4K12. TUNEL assay and Hoechst 33342 staining revealed that the percentage of apoptotic cells in blastocysts was significantly lower in PCI-24781-treated SCNT embryos than in untreated embryos. Also, PCI-24781-treated embryos were transferred into three surrogate sows, one of whom became pregnant and two fetuses developed.


CONCLUSIONS
PCI-24781 improves nuclear reprogramming and the developmental potential of pig SCNT embryos.
Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, 133000, Jilin, China.
Long
L
Jin
Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, 133000, Jilin, China.
Hai-Ying
HY
Zhu
Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, 133000, Jilin, China.
Qing
Q
Guo
Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, 133000, Jilin, China.
Xiao-Chen
XC
Li
Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, 133000, Jilin, China.
Yu-Chen
YC
Zhang
Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, 133000, Jilin, China.
Guang-Lei
GL
Zhang
Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, 133000, Jilin, China.
Xiao-Xu
XX
Xing
Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, 133000, Jilin, China.
Mei-Fu
MF
Xuan
Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, 133000, Jilin, China.
Qi-Rong
QR
Luo
Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, 133000, Jilin, China. yinxj33@msn.com.
Xi-Jun
XJ
Yin
Jilin Provincial Key Laboratory of Transgenic Animal and Embryo Engineering, Yanbian University, Yanji, 133000, Jilin, China. kangjindan@hotmail.com.
Jin-Dan
JD
Kang
D000818:Animals; D001572:Benzofurans / Q000494:pharmacology*; D004622:Embryo, Mammalian / Q000187:drug effects / Q000378:metabolism; D047108:Embryonic Development / Q000187:drug effects / Q000235:genetics; D005260:Female; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D006877:Hydroxamic Acids / Q000494:pharmacology*; D053652:Nuclear Transfer Techniques; D011247:Pregnancy; D013552:Swine
D001572:Benzofurans; D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; C512352:abexinostat
Abexinostat; Histone deacetylase inhibitors (HDACi); In vitro development; PCI-24781; Pig; Somatic cell nuclear transfer (SCNT)