Rapid Detection of the Apoptosis Related Genes (BCL-2, BAD, BAX) in 12 Hours 1,25 (OH)2D3 Treated HL-60 Cells Using Real-Time Quantitative RT-PCR.
HL-60 cell line offers an interesting model to study apoptosis. Our aim was to detect the expressions of three apoptotic genes (BCL-2, BAD and BAX) using Real-Time quantitative polymerase chain reaction (PCR). HL-60 cells were incubated with 1,25 (OH)2D3 (5 x 10-8 M). RNA portions were isolated and LightCycler analysis were performed on cDNA samples. 1,25 (OH)2D3 incubated cell portions were compared to nontreated portions. We observed down-regulated levels of three genes at the end of the 12 hours. Gene expression ratios were 0.98 for BAX, 0.41 for BAD, 0.81 for BCL-2. For the first time, this study represents the expression levels of these three apoptosis related genes together during early phase (12 hours) of 1,25 (OH)2D3 treatment of HL-60 cells. Data indicate that expression differences of these apoptosis genes take place so early in exposure to 1,25 (OH)2D3. This optimised strategy would be used in rapid understanding of similar apoptotic deregulations.
Hakan
H
Savlı
Saadet
S
Özdemir
Balint
B
Nagy