Antioxidant and antimutagenic potential of Psidium guajava leaf extracts.
Fruits, vegetables and medicinal herbs rich in phenolics antioxidants contribute toward reduced risk of age-related diseases and cancer. In this study, Psidium guajava leaf extract was fractionated in various organic solvents viz. petroleum ether, benzene, ethyl acetate, ethanl and methanol and tested for their antioxidant and antimutagenic properties. Methanolic fraction showed maximum antioxidant activity comparable to ascorbic acid and butylated hydroxyl toluene (BHT) as tested by DPPH free radical scavenging, phosphomolybdenum, FRAP (Fe3 + reducing power) and CUPRAC (cupric ions (Cu2+) reducing ability) assays. The fraction was analyzed for antimutagenic activities against sodium azide (NaN3), methylmethane sulfonate (MMS), 2-aminofluorene (2AF) and benzo(a)pyrene (BP) in Ames Salmonella tester strains. The methanol extracted fraction at 80 μg/ml concentration inhibited above 70% mutagenicity. Further, phytochemical analysis of methanol fraction that was found to be most active revealed the presence of nine major compounds by gas chromatography-mass spectrometry (GC-MS). This data suggests that guava contains high amount of phenolics responsible for broad-spectrum antimutagenic and antioxidant properties in vitro and could be potential candidates to be explored as modern phytomedicine.
a Department of Agricultural Microbiology , Aligarh Muslim University , Aligarh , UP , India and.|b James Graham Brown Cancer Center, University of Louisville , Louisville , KY , USA.
Maryam
M
Zahin
a Department of Agricultural Microbiology , Aligarh Muslim University , Aligarh , UP , India and.
Iqbal
I
Ahmad
a Department of Agricultural Microbiology , Aligarh Muslim University , Aligarh , UP , India and.|b James Graham Brown Cancer Center, University of Louisville , Louisville , KY , USA.
Farrukh
F
Aqil
D016587:Antimutagenic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D003300:Copper / Q000737:chemistry; D004269:DNA, Bacterial / Q000187:drug effects / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D005292:Ferricyanides / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D008982:Molybdenum / Q000737:chemistry; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity; D009154:Mutation / Q000187:drug effects*; D010084:Oxidation-Reduction; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology; D008517:Phytotherapy; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D010946:Plants, Medicinal; D031647:Psidium / Q000737:chemistry*; D012475:Salmonella / Q000187:drug effects / Q000235:genetics; D012997:Solvents / Q000737:chemistry
D016587:Antimutagenic Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D004269:DNA, Bacterial; D005292:Ferricyanides; D009153:Mutagens; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D012997:Solvents; D003300:Copper; D008982:Molybdenum; C004931:1,1-diphenyl-2-picrylhydrazyl; C029892:cupric chloride; C028033:potassium ferricyanide
Ames test; GC/MS analysis; Psidium guajava; antimutagenic activity; antioxidant activity; methanolic extract