Analysis of β2-agonists in cattle hair samples using a rapid UHPLC-ESI-MS/MS method.
A simple and efficient method was developed for simultaneous analysis of five illegal residual β2-agonists in cattle hair. β2-Agonists were quantified by ultra high performance liquid chromatography coupled with electrospray ionisation tandem mass spectrometry operating in positive multiple-reaction monitoring mode. The method was validated as quantitative confirmatory method according to the EU Decision 2002/657/EC: instrumental linearity, specificity, precision, recovery, decision limit (CCα) and detection capability (CCβ) were evaluated. The recovery were greater than 90% and the method appeared suitable for the control of these β2-agonists in cattle hair samples with LOQ values between 4.9 and 5.5 μg/kg. This method could represent a simple and cheap approach to confirm β2-agonists contamination of cattle for feeding in a not invasive way and before slaughter operations.
a Department of Biological, Chemical and Pharmaceutical Science and Technology (STEBICEF) , University of Palermo , Palermo , Italy.
Vita
V
Di Stefano
b CGA, UNINETLAB , University of Palermo , Palermo , Italy.
Rosa
R
Pitonzo
c Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri" , Palermo , Italy.
Vita
V
Giaccone
c Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri" , Palermo , Italy.
Angelina
A
Alongi
c Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri" , Palermo , Italy.
Andrea
A
Macaluso
d Dipartimento di Scienze dell'Ambiente , della Sicurezza, del Territorio, degli Alimenti e della Salute (SASTAS) , Messina , Italy.
Nicola
N
Cicero
a Department of Biological, Chemical and Pharmaceutical Science and Technology (STEBICEF) , University of Palermo , Palermo , Italy.
Gabriella
G
Cancemi
c Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri" , Palermo , Italy.
Vincenzo
V
Ferrantelli
D000318:Adrenergic beta-Agonists / Q000032:analysis*; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006197:Hair / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000318:Adrenergic beta-Agonists
Commission Decision 2002/657/EC; UHPLC–ESI–MS/MS; cattle hair; β2-agonists