High Quality Independent From a Donor: Human Amniotic Fluid Derived Stem Cells-A Practical Analysis Based on 165 Clinical Cases.
The aim of the study was to extend the potential use of human stem cells isolated from amniotic fluid in medical applications by confirming their high homogeneity and quality. Amniotic fluid samples were collected during amniocentesis from 165 women during pregnancy. The proliferation rate, clonogenicity, karyotype, aging process, pluripotent cell markers, expression of surface markers, and the potential to differentiate into adipose, bone and cartilage cells of hAFSCs were analyzed. Obtained results revealed that mesenchymal stem cells could be derived successfully from amniotic fluid, which exhibit properties comparable with MSCs of other origins. It is the first study, in which such a large group of patients was involved. Comprehensive statistical and biological analysis were conducted some of which clearly being innovative in relation to human amniotic fluid-derived stem cells. J. Cell. Biochem. 118: 116-126, 2017. © 2016 Wiley Periodicals, Inc.
Department of Tissue Engineering, Nicolaus Copernicus University, Bydgoszcz, 85-092, Poland.
Anna
A
Bajek
Department of Tissue Engineering, Nicolaus Copernicus University, Bydgoszcz, 85-092, Poland.
Joanna
J
Olkowska
Department of Obstetrics and Gynecology, Nicolaus Copernicus University, Bydgoszcz, 85-168, Poland.
Małgorzata
M
Walentowicz-Sadłecka
Department of Obstetrics and Gynecology, Nicolaus Copernicus University, Bydgoszcz, 85-168, Poland.
Paweł
P
Walentowicz
Department of Obstetrics and Gynecology, Nicolaus Copernicus University, Bydgoszcz, 85-168, Poland.
Paweł
P
Sadłecki
Department of Obstetrics and Gynecology, Nicolaus Copernicus University, Bydgoszcz, 85-168, Poland.
Marek
M
Grabiec
Department of Clinical Pathomorphology, Nicolaus Copernicus University, Bydgoszcz, 85-094, Poland.
Magdalena
M
Bodnar
Department of Clinical Pathomorphology, Nicolaus Copernicus University, Bydgoszcz, 85-094, Poland.
Andrzej
A
Marszałek
Department of Experimental Oncology, Nicolaus Copernicus University, Bydgoszcz, 85-094, Poland.
Robert
R
Dębski
Department of Biochemistry, Nicolaus Copernicus University, Toruń, 87-100, Poland.
Dorota
D
Porowińska
Department of Biochemistry, Nicolaus Copernicus University, Toruń, 87-100, Poland.
Joanna
J
Czarnecka
Department of Tissue Engineering, Nicolaus Copernicus University, Bydgoszcz, 85-092, Poland.
Łukasz
Ł
Kaźmierski
Department of Tissue Engineering, Nicolaus Copernicus University, Bydgoszcz, 85-092, Poland.|Department of Urology, Nicolaus Copernicus Hospital, Toruń, 87-100, Poland.
Tomasz
T
Drewa
D000293:Adolescent; D000328:Adult; D000653:Amniotic Fluid / Q000166:cytology / Q000378:metabolism; D000943:Antigens, Differentiation / Q000096:biosynthesis; D049109:Cell Proliferation / Q000502:physiology; D002469:Cell Separation / Q000379:methods* / Q000592:standards; D016922:Cellular Senescence / Q000502:physiology; D005260:Female; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D008875:Middle Aged; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism; D011247:Pregnancy
D000943:Antigens, Differentiation
AMNIOTIC FLUID; BIOLOGICAL EVALUATION; STEM CELLS