3-Chloro-1,2-propanediol biodegradation by Ca-alginate immobilized Pseudomonas putida DSM 437 cells applying different processes: mass transfer effects.
3-Chloro-1,2-propanediol (3-CPD) biodegradation by Ca-alginate immobilized Pseudomonas putida cells was performed in batch system, continuous stirred tank reactor (CSTR), and packed-bed reactor (PBR). Batch system exhibited higher biodegradation rates and 3-CPD uptakes compared to CSTR and PBR. The two continuous systems (CSTR and PBR) when compared at 200 mg/L 3-CPD in the inlet exhibited the same removal of 3-CPD at steady state. External mass-transfer limitations are found negligible at all systems examined, since the observable modulus for external mass transfer Ω ≪ 1 and the Biot number Bi > 1. Intra-particle diffusion resistance had a significant effect on 3-CPD biodegradation in all systems studied, but to a different extent. Thiele modulus was in the range of 2.5 in batch system, but it was increased at 11 when increasing cell loading in the beads, thus lowering significantly the respective effectiveness factor. Comparing the systems at the same cell loading in the beads PBR was less affected by internal diffusional limitations compared to CSTR and batch system, and, as a result, exhibited the highest overall effectiveness factor.
Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Str., Zografou Campus, 157 80, Zografou, Greece.
Aikaterini
A
Konti
Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Str., Zografou Campus, 157 80, Zografou, Greece.
Diomi
D
Mamma
Microbial Biotechnology Unit, Sector of Botany, Department of Biology, National and Kapodistrian University of Athens, Athens, Zografou, Greece.
Dimitios G
DG
Hatzinikolaou
Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Str., Zografou Campus, 157 80, Zografou, Greece. kekos@chemeng.ntua.gr.
Dimitris
D
Kekos
D000464:Alginates / Q000737:chemistry*; D018914:Cells, Immobilized / Q000378:metabolism; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D016958:Pseudomonas putida / Q000378:metabolism*; D000517:alpha-Chlorohydrin / Q000378:metabolism*
D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D000517:alpha-Chlorohydrin
3-Chloro-1,2-propanediol; Biodegradation; Continuous stirred tank reactor; Immobilized cells; Mass transfer; Packed-bed bioreactor