Application of arteether-loaded polyurethane nanomicelles to induce immune response in breast cancer model.
To concentrate a potent anticancer drug (Arteether) in tumor microenvironment, we encapsulated it in biodegradable and pH sensitive polyurethane (PU) nanomicelles (NMs). The nanocomplex was characterized by Fourier transform infrared (FTIR), dynamic light scattering (DLS). The loading capacity and release profile in pH of 5.4 and 7.4 were considered. The cytotoxicity effect was evaluated in vitro and in vivo settings. The level of IFN-γ and IL-4 cytokines of mice splenocytes were assessed by enzyme-linked immunosorbent assay (ELISA). The nanocomplex showed negative zeta charge of -26.2 mV, size of 42.30 nm and high loading capacity (92%). Release profile showed a faster rate of drug liberation at pH 5.4 as compared to that of pH 7.4. It indicated significant inhibitory effect on the growth of 4T1 cell line and increased IFN-γ level.
a Department of Immunology, Faculty of Medical Sciences , Tabriz University of Medical Sciences , Tabriz , Iran.
Mona
M
Jabbarzadegan
b Department of Immunology, Faculty of Medical Sciences , Tarbiat Modares University , Tehran , Iran.
Hajar
H
Rajayi
b Department of Immunology, Faculty of Medical Sciences , Tarbiat Modares University , Tehran , Iran.
Mirza Ali
MA
Mofazzal Jahromi
c Polyurethane Department, Faculty of Polymer Science , Iran Polymer and Petrochemical Institute , Tehran , Iran.
Hamid
H
Yeganeh
a Department of Immunology, Faculty of Medical Sciences , Tabriz University of Medical Sciences , Tabriz , Iran.|d Immunology Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.
Mehdi
M
Yousefi
b Department of Immunology, Faculty of Medical Sciences , Tarbiat Modares University , Tehran , Iran.
Zuhair
Z
Muhammad Hassan
a Department of Immunology, Faculty of Medical Sciences , Tabriz University of Medical Sciences , Tabriz , Iran.|d Immunology Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.
Jafar
J
Majidi
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D037621:Artemisinins / Q000737:chemistry* / Q000494:pharmacology* / Q000627:therapeutic use; D001943:Breast Neoplasms / Q000188:drug therapy / Q000276:immunology* / Q000473:pathology; D045744:Cell Line, Tumor; D016207:Cytokines / Q000378:metabolism; D004337:Drug Carriers / Q000737:chemistry*; D065546:Drug Liberation; D005260:Female; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D008325:Mammary Neoplasms, Experimental / Q000188:drug therapy / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008823:Micelles; D053758:Nanoparticles / Q000737:chemistry*; D011140:Polyurethanes / Q000737:chemistry*; D012995:Solubility; D047368:Tumor Burden / Q000187:drug effects / Q000276:immunology; D014867:Water / Q000737:chemistry
D000970:Antineoplastic Agents; D037621:Artemisinins; D016207:Cytokines; D004337:Drug Carriers; D008823:Micelles; D011140:Polyurethanes; D014867:Water; C055141:artemotil
4T1; Cancer therapy; immunostimulator; nanocarrier