Ceruloplasmin decreases respiratory burst reaction during pregnancy.
Testing of pregnant women reveals weakening of neutrophil-mediated effector functions, such as reactive oxygen species generation. This study provides data confirming the phenomenon, gained through application of the flow cytometry technique. Key factors influencing neutrophil functional activity in blood plasma of pregnant women have not been detected so far. At the same time, concentration of ceruloplasmin - a copper-containing glycoprotein - is known to increase in blood significantly during pregnancy. We observed the negative correlation between ceruloplasmin concentration in blood plasma of pregnant women and the intensity of respiratory burst of neutrophils. Fractionation of plasma using gel-filtration revealed that ceruloplasmin-containing fraction demonstrated suppression of the respiratory burst reaction. Partial elimination of ceruloplasmin from the blood of pregnant women, performed with the help of specific antibodies and followed by immunoprecipitation, leads to an increased respiratory burst reaction. On the contrary, addition of ceruloplasmin to blood samples of healthy donors noticeably decreases the respiratory burst reaction. The results presented prove that change in ceruloplasmin level in plasma is necessary and sufficient for modulating the ability of neutrophils to produce reactive oxygen species during pregnancy.
a Division of Molecular and Radiation Biophysics , SFBI "Petersburg Nuclear Physics Institute" (National Research Center "Kurchatov Institute") , Gatchina , Russia ;
Elena Y
EY
Varfolomeeva
a Division of Molecular and Radiation Biophysics , SFBI "Petersburg Nuclear Physics Institute" (National Research Center "Kurchatov Institute") , Gatchina , Russia ;
Elena V
EV
Semenova
b Department of Molecular Genetics , FSBSI "Institute of Experimental Medicine" , St. Petersburg , Russia ;|c Chair of Fundamental Problems of Medicine, Saint-Petersburg State University , St. Petersburg , Russia.
Alexey V
AV
Sokolov
a Division of Molecular and Radiation Biophysics , SFBI "Petersburg Nuclear Physics Institute" (National Research Center "Kurchatov Institute") , Gatchina , Russia ;
Kirill D
KD
Aplin
a Division of Molecular and Radiation Biophysics , SFBI "Petersburg Nuclear Physics Institute" (National Research Center "Kurchatov Institute") , Gatchina , Russia ;
Kseniya E
KE
Timofeeva
b Department of Molecular Genetics , FSBSI "Institute of Experimental Medicine" , St. Petersburg , Russia ;|c Chair of Fundamental Problems of Medicine, Saint-Petersburg State University , St. Petersburg , Russia.
Vadim B
VB
Vasilyev
a Division of Molecular and Radiation Biophysics , SFBI "Petersburg Nuclear Physics Institute" (National Research Center "Kurchatov Institute") , Gatchina , Russia ;
Michael V
MV
Filatov
D002570:Ceruloplasmin / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D009504:Neutrophils / Q000378:metabolism*; D011247:Pregnancy; D016897:Respiratory Burst / Q000502:physiology*
D002570:Ceruloplasmin
Ceruloplasmin; flow cytometry; neutrophils; pregnancy; respiratory burst reaction