Effects of BDNF-Transfected BMSCs on Neural Functional Recovery and Synaptophysin Expression in Rats with Cerebral Infarction.
The purpose of this study was to investigate the effects of brain-derived neurotrophic factor (BDNF)-transfected bone marrow mesenchymal stem cells (BMSCs) on neural functional recovery and synaptophysin expression in rats with cerebral infarction (CI). A total of 120 healthy Sprague Dawley rats were randomly divided into sham group, control group, and model group. Craniotomy was conducted and neurological function defect scoring was used to verify the model. BDNF containing recombinant plasmid was transfected into rat BMSCs, which was verified by flow cytometry and Western Blot. After injection of the transfected BMSCs, neural functional recovery of the CI rats and synaptophysin expression were measured. After the CI rat model was established, magnetic resonance (MR) imaging, 2, 3, 5- triphenyl tetrazolium chloride (TTC) staining, and the neurological function defect scoring determined the success of the model. CD34 (-), CD45 (-), CD29 (+), and CD90 (+) cells detected showed that the obtained BMSCs have high purity. BDNF protein was highly expressed in the BMSCs successfully transfected with the recombinant plasmid. Balance beam walking score, rotating bar walking score, and screen test score were significantly lower, while synaptophysin expression was higher in the BDNF model group than those in the non-BDNF model group and sham group with time extension. BDNF can increase synaptic plasticity and neurogenesis and have a promotional role in neural functional recovery and synaptophysin expression in rats with CI. BDNF-transfected BMSCs may therefore have better treatment efficacy for CI clinically.
Department of Neurosurgery, No. 105 Hospital of PLA, No. 424 Changjiang West Road, Hefei, 230031, Anhui, People's Republic of China. zymhf2966@163.com.
Yongming
Y
Zhang
Department of Neurosurgery, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, Guangdong, People's Republic of China.
Binghui
B
Qiu
Department of Neurosurgery, No. 105 Hospital of PLA, No. 424 Changjiang West Road, Hefei, 230031, Anhui, People's Republic of China.
Jinbiao
J
Wang
Department of Neurosurgery, No. 105 Hospital of PLA, No. 424 Changjiang West Road, Hefei, 230031, Anhui, People's Republic of China.
Yi
Y
Yao
Department of Neurosurgery, No. 105 Hospital of PLA, No. 424 Changjiang West Road, Hefei, 230031, Anhui, People's Republic of China.
Chunlin
C
Wang
Department of Neurosurgery, No. 105 Hospital of PLA, No. 424 Changjiang West Road, Hefei, 230031, Anhui, People's Republic of China.
Jiachuan
J
Liu
D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism*; D048430:Cell Shape; D002544:Cerebral Infarction / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology* / Q000628:therapy*; D004195:Disease Models, Animal; D008279:Magnetic Resonance Imaging; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009417:Nerve Tissue / Q000473:pathology / Q000503:physiopathology; D010957:Plasmids / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function; D016708:Synaptophysin / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
D019208:Brain-Derived Neurotrophic Factor; C489787:MAP2 protein, rat; D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger; D016708:Synaptophysin
Bone marrow mesenchymal stem cells; Brain-derived neurotrophic factor; Cerebral infarction; Flow cytometry; Immunohistochemistry; Neural functional recovery; Synaptophysin; Transfection