Cytomegalovirus (CMV) Enzyme-Linked Immunosorbent Spot Assay but Not CMV QuantiFERON Assay Is a Novel Biomarker To Determine Risk of Congenital CMV Infection in Pregnant Women.
Cytomegalovirus (CMV) enzyme-linked immunosorbent spot (ELISPOT) and CMV QuantiFERON assays were examined as potential biomarkers predictive of congenital CMV (cCMV) transmission. Fifty-seven pregnant women with primary CMV infection and 23 with nonprimary CMV infection were recruited in the study. Maternal age, CMV IgG avidity, viremia, and viruria were also included among the potential predictors. Spearman's statistical correlation analysis revealed a positive correlation between the CMV ELISPOT and CMV QuantiFERON assay results (P < 0.001), but only the CMV ELISPOT assay correlated with cCMV (P < 0.001). cCMV was positively correlated with maternal viremia and viruria (P < 0.05) and negatively correlated with CMV IgG avidity (P < 0.01). Maternal age and CMV QuantiFERON assay results were not statistically associated with cCMV. CMV-specific cell-mediated immunity detected by the CMV ELISPOT assay plays a critical role in cCMV.
Department of Molecular Medicine, University of Padua, Padua, Italy.
Gabriella
G
Forner
Department of Molecular Medicine, University of Padua, Padua, Italy.
Alda
A
Saldan
Department of Molecular Medicine, University of Padua, Padua, Italy.
Carlo
C
Mengoli
Padua Reference Center for Infections in Pregnancy, Padua General Hospital, Padua, Italy.
Nadia
N
Gussetti
Department of Molecular Medicine, University of Padua, Padua, Italy.
Giorgio
G
Palù
Department of Molecular Medicine, University of Padua, Padua, Italy davide.abate@unipd.it.
Davide
D
Abate
D000293:Adolescent; D000328:Adult; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D058501:Enzyme-Linked Immunospot Assay / Q000379:methods*; D005260:Female; D006801:Humans; D059425:Interferon-gamma Release Tests / Q000379:methods*; D008875:Middle Aged; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis*; D055815:Young Adult