Hydrogen inhibits cytotrophoblast cells apoptosis in hypertensive disorders complicating pregnancy.
Hypertensive disorders complicating pregnancy (HDCP) is one of the most serious medical disorders during pregnancy. Hydrogen is a therapeutic antioxidant and used to treat HDCP effectively. However, the molecular mechanism about the effect of hydrogen on HDCP still remains unclear. In this study, we found ROS content in HDCP group was significantly higher than that in the control and was reduced markedly in the presence of 100μmol/L hydrogen. IL6, Caspase3, Bax1, P-JAK2, P-Stat3 and P-p38 expression was much higher than the control, and was notably decreasedby the application of 100μmol/L hydrogen. Bcl2 expression in HDCP group was notably lower than the control and was increased by 100 μmol/L hydrogen. The apoptosis rate of cytotrophoblast cells was decreased, andratio of cytotrophoblast cells at G1 and G2 phase was increased and decreased by hydrogen, respectively. All those data indicated a potential molecular mechanism of hydrogen-mediated treatment in HDCP.
Tongji University School of Medicine Shanghai First Maternity and Infant Hospital Shanghai China.
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Guo
Tongji Hospital of Tongji University Tongji University School of Medicine, Department of Obstetrics and Gynecology Shanghai China.
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Guan
Tongji Hospital of Tongji University Tongji University School of Medicine, Department of Obstetrics and Gynecology Shanghai China.
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Li
Tongji Hospital of Tongji University Tongji University School of Medicine, Department of Obstetrics and Gynecology Shanghai China xiang1_yang@sina.com.
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Yang
D017209:Apoptosis / Q000187:drug effects*; D002452:Cell Count; D005260:Female; D016193:G1 Phase / Q000187:drug effects; D006801:Humans; D006859:Hydrogen / Q000494:pharmacology*; D046110:Hypertension, Pregnancy-Induced / Q000201:enzymology / Q000473:pathology*; D020935:MAP Kinase Signaling System / Q000187:drug effects; D011247:Pregnancy; D017382:Reactive Oxygen Species / Q000378:metabolism; D014327:Trophoblasts / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*
D017382:Reactive Oxygen Species; D006859:Hydrogen