Elevated levels of miR-210 correlate with anemia in β-thalassemia/HbE patients.
Ineffective erythropoiesis in β-thalassemia patients is caused by the premature death of red blood cell precursors due to excess α-globin chains. As a consequence, patients develop chronic anemia and hypoxia. Upregulation of miR-210, a hypoxia-induced miRNA, has been shown to regulate globin gene expression and erythroid differentiation in β-thalassemia/HbE erythroid progenitor cell culture. The present study examined whether the expression of miR-210 in circulation reflects the anemic condition in these patients. The level of miR-210 expression was directly examined from red blood cells and plasma of β-thalassemia/HbE patients. Transferrin receptor, a marker of erythropoiesis activity, was also analyzed. Increased expression of both red blood cells and plasma miR-210 as well as elevated levels of serum transferrin receptor in β-thalassemia/HbE patients were observed when compared to those of normal individuals (p < 0.05). In addition, red blood cell miR-210 level was inversely correlated with hemoglobin levels (r = -0.7054, p < 0.01) and hematocrit (r = -0.6017, p < 0.05). The higher expression of miR-210 in these patients may be the consequence of hypoxia occurring from the lower hemoglobin level. Thus, analysis of red blood cell miR-210 may be useful as a method for assessing hypoxia in β-thalassemia patients.
Thalassemia Research Center, Institute of Molecular Biosciences, Mahidol University, 25/25 Phuttamonthon 4 Rd, Salaya, Nakhon Pathom, 73170, Thailand.|Molecular Medicine Graduate Program, Faculty of Science, Mahidol University, Bangkok, Thailand.
Panjaree
P
Siwaponanan
Thalassemia Research Center, Institute of Molecular Biosciences, Mahidol University, 25/25 Phuttamonthon 4 Rd, Salaya, Nakhon Pathom, 73170, Thailand.
Suthat
S
Fucharoen
Thalassemia Research Center, Institute of Molecular Biosciences, Mahidol University, 25/25 Phuttamonthon 4 Rd, Salaya, Nakhon Pathom, 73170, Thailand.
Pornpan
P
Sirankapracha
Thalassemia Research Center, Institute of Molecular Biosciences, Mahidol University, 25/25 Phuttamonthon 4 Rd, Salaya, Nakhon Pathom, 73170, Thailand.
Pranee
P
Winichagoon
Division of Medical Technology, Department of Health Sciences, Faculty of Medical Sciences, Kyushu University, Fukuoka, Japan.|Department of Medical Technology and Sciences, International University of Health and Welfare, Ohkawa, Japan.
Tsukuru
T
Umemura
Thalassemia Research Center, Institute of Molecular Biosciences, Mahidol University, 25/25 Phuttamonthon 4 Rd, Salaya, Nakhon Pathom, 73170, Thailand. saovaros.sva@mahidol.ac.th.|Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand. saovaros.sva@mahidol.ac.th.
Saovaros
S
Svasti
D000740:Anemia / Q000097:blood / Q000175:diagnosis*; D004912:Erythrocytes / Q000378:metabolism; D004920:Erythropoiesis; D006446:Hemoglobin E / Q000032:analysis; D006801:Humans; D000860:Hypoxia / Q000175:diagnosis; D035683:MicroRNAs / Q000097:blood*; D011990:Receptors, Transferrin / Q000097:blood; D017086:beta-Thalassemia / Q000175:diagnosis
C543800:MIRN210 microRNA, human; D035683:MicroRNAs; D011990:Receptors, Transferrin; D006446:Hemoglobin E
Hemoglobin; Hypoxia; Thalassemia; miR-210