Propionibacterium namnetense sp. nov., isolated from a human bone infection.
A polyphasic taxonomic study was performed on two Gram-positive-staining, anaerobic, pleomorphic, rod-shaped strains isolated from human bone and tissue samples. Sequencing of the 16S rRNA genes revealed that the strains belong to a novel species within the genus Propionibacterium, most closely related to Propionibacterium acnes subsp. acnes and Propionibacterium acnes subsp. elongatum with similarity values of 98.4 % and 98.1 %, respectively. In addition, protein-coding genes for rpoB, recA and gyrB clearly separated the novel organism from all species and subspecies of the genus Propionibacterium. However, a DNA-DNA hybridization analysis between the novel organism and the type strain P. acnes ATCC 6919T revealed a value of only 61.1 %. Furthermore, whole genome analysis using the program OrthoANI gave a value of 88.5 %, which is significantly below the cut-off value of 95 % for species delineation. The major fatty acids were iso-C15 : 0, anteiso-C15 : 0 and iso-C17 : 0. The DNA G+C content of the type strain was 59.7 mol%. When taken collectively, phenotypic, molecular genetic, chemotaxonomic and phylogenetic information demonstrate that the organism represents a distinct, albeit close relative of P. acnes On the basis of the results presented, the organism represents a novel member of the genus Propionibacterium for which the name Propionibacterium namnetense sp. nov. is proposed. The type strain is NTS 31307302T (=DSM 29427T=CCUG 66358T).
Bacteriology and Hygiene department, Nantes University Hospital, 9 quai Moncousu, 44093 Nantes, France.|EA3826, Laboratory of clinical and experimental therapeutics of infections, Nantes Medicine School, 1 rue Gaston Veil, 44000 Nantes, France.
Guillaume Ghislain
GG
Aubin
Bacteriology and Hygiene department, Nantes University Hospital, 9 quai Moncousu, 44093 Nantes, France.
Pascale
P
Bémer
Institut de Recherche en Santé de l'Université de Nantes INSERM U892 - CNRS 6299 CRCNA Team 13: Nuclear Oncology Research Group: Engineering of Recognition, Nantes, France.
Stanimir
S
Kambarev
Department of Microbiology and Plant Biology, University of Oklahoma, 770 Van Vleet Oval, Norman, OK 73019, USA.
Nisha B
NB
Patel
Microbiology Laboratory, Saint-Nazaire Hospital, Saint-Nazaire, France.
Olivier
O
Lemenand
Bacteriology and Hygiene department, Nantes University Hospital, 9 quai Moncousu, 44093 Nantes, France.|EA3826, Laboratory of clinical and experimental therapeutics of infections, Nantes Medicine School, 1 rue Gaston Veil, 44000 Nantes, France.
Jocelyne
J
Caillon
Department of Microbiology and Plant Biology, University of Oklahoma, 770 Van Vleet Oval, Norman, OK 73019, USA.
Paul A
PA
Lawson
Bacteriology and Hygiene department, Nantes University Hospital, 9 quai Moncousu, 44093 Nantes, France.|Institut de Recherche en Santé de l'Université de Nantes INSERM U892 - CNRS 6299 CRCNA - Centre de Cancérologie et d'Immunologie Nantes Angers Université de Nantes Nantes France Team 2: Clinical and translational research in skin cancer, Nantes, France.
Stéphane
S
Corvec
D015373:Bacterial Typing Techniques; D001482:Base Composition; D001850:Bone Diseases, Infectious / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005602:France; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011424:Propionibacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D055815:Young Adult
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S