Lysophosphatidylcholine plays critical role in allergic airway disease manifestation.
Phospholipase A2 (sPLA2), pivotal for allergic and inflammatory response, hydrolyses phosphatidylcholine (PC) to lysophosphatidylcholine (LPC). In present study, the role of LPC in allergic airway disease manifestation was studied using mouse model. Balb/c mice were immunized using cockroach extract (CE) and LPC release was blocked by sPLA2 inhibitor. Airway hyperresponse (AHR), lung-histology, total and differential leukocyte count (TLC&DLC), Th2 type cytokines, sPLA2 activity and LPC levels in bronchoalveolar lavage fluid (BALF) were measured. Exogenous LPC was given to the mice with or without CE sensitization, to demonstrate its role in allergic airway disease manifestation. Anti-CD1d antibody was given to study the involvement of natural killer T (NKT) cells in LPC induced response. AHR, lung-inflammation, TLC, DLC, Th2 type cytokines, sPLA2 activity and LPC levels were increased on CE challenge. sPLA2 activity and LPC release was blocked by sPLA2-inhibitor, which decreased AHR, and inflammatory parameters. Exogenous LPC with or without CE sensitization increased above parameters. CE challenge or LPC exposure increased LY49C(+)TCRβ(+) NKT cells in BALF and spleen, which was reduced by anti-CD1d antibody, accompanied with reduction in AHR and allergic airway inflammation parameters. Conclusively, LPC induces allergic airway disease manifestation and it does so probably via CD1d-restricted LY49C(+)TCRβ(+) NKT cells.
Allergy and Immunology Section, CSIR-Institute of Genomics and Integrative Biology, Delhi.|Department of Biotechnology, University of Pune, Ganeshkhind, Pune 411 007, India.
Preeti
P
Bansal
Department of Pulmonary Medicine,V.P.Chest Institue, Delhi University, Delhi.
Shailendera Nath
SN
Gaur
Allergy and Immunology Section, CSIR-Institute of Genomics and Integrative Biology, Delhi.
Naveen
N
Arora
D000818:Animals; D055610:Antigens, CD1d / Q000276:immunology; D001249:Asthma / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D003058:Cockroaches / Q000276:immunology; D016207:Cytokines / Q000276:immunology; D005260:Female; D007249:Inflammation / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology; D007958:Leukocyte Count / Q000379:methods; D008168:Lung / Q000276:immunology / Q000378:metabolism*; D008244:Lysophosphatidylcholines / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D054467:Phospholipases A2 / Q000276:immunology; D012130:Respiratory Hypersensitivity / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology
D055610:Antigens, CD1d; D016207:Cytokines; D008244:Lysophosphatidylcholines; D054467:Phospholipases A2