Photodegradation of retinal bisretinoids in mouse models and implications for macular degeneration.
Adducts of retinaldehyde (bisretinoids) form nonenzymatically in photoreceptor cells and accumulate in retinal pigment epithelial (RPE) cells as lipofuscin; these fluorophores are implicated in the pathogenesis of inherited and age-related macular degeneration (AMD). Here we demonstrate that bisretinoid photodegradation is ongoing in the eye. High-performance liquid chromatography (HPLC) analysis of eyes of dark-reared and cyclic light-reared wild-type mice, together with comparisons of pigmented versus albino mice, revealed a relationship between intraocular light and reduced levels of the bisretinoids A2E and A2-glycero-phosphoethanolamine (A2-GPE). Analysis of the bisretinoids A2E, A2-GPE, A2-dihydropyridine-phosphatidylethanolamine (A2-DHP-PE), and all-trans-retinal dimer-phosphatidylethanolamine (all-trans-retinal dimer-PE) also decreases in albino Abca4(-/-) mice reared in cyclic light compared with darkness. In albino Abca4(-/-) mice receiving a diet supplemented with the antioxidant vitamin E, higher levels of RPE bisretinoid were evidenced by HPLC analysis and quantitation of fundus autofluorescence; this effect is consistent with photooxidative processes known to precede bisretinoid degradation. Amelioration of outer nuclear layer thinning indicated that vitamin E treatment protected photoreceptor cells. Conversely, in-cage exposure to short-wavelength light resulted in reduced fundus autofluorescence, decreased HPLC-quantified A2E, outer nuclear layer thinning, and increased methylglyoxal (MG)-adducted protein. MG was also released upon bisretinoid photodegradation in cells. We suggest that the lower levels of these diretinal adducts in cyclic light-reared and albino mice reflect photodegradative loss of bisretinoid. These mechanisms may underlie associations among AMD risk, oxidative mechanisms, and lifetime light exposure.
Department of Ophthalmology, Columbia University Medical Center, New York, NY 10032;
Keiko
K
Ueda
Department of Ophthalmology, Columbia University Medical Center, New York, NY 10032;
Jin
J
Zhao
Department of Ophthalmology, Columbia University Medical Center, New York, NY 10032;
Hye Jin
HJ
Kim
Department of Ophthalmology, Columbia University Medical Center, New York, NY 10032; Department of Pathology and Cell Biology, Columbia University Medical Center, New York, NY 10032 jrs88@cumc.columbia.edu.
Janet R
JR
Sparrow
D000818:Animals; D003624:Darkness; D008027:Light; D008268:Macular Degeneration / Q000209:etiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010084:Oxidation-Reduction; D012160:Retina / Q000378:metabolism / Q000528:radiation effects*; D012176:Retinoids / Q000378:metabolism*
D012176:Retinoids
bisretinoid; macular degeneration; retina; retinal pigment epithelium; visual cycle