Vibrio ishigakensis sp. nov., in Halioticoli clade isolated from seawater in Okinawa coral reef area, Japan.
Five novel strains showing non-motile, alginolytic, halophilic and fermentative features were isolated from seawater samples off Okinawa in coral reef areas. These strains were characterized by an advanced polyphasic taxonomy including genome based taxonomy using multilocus sequence analysis (MLSA) and in silico DNA-DNA similarity (in silico DDH). Phylogenetic analyses on the basis of 16S rRNA gene sequences revealed that the isolates could be assigned to the genus Vibrio, however they were not allocated into any distinct cluster with known Vibrionaceae species. MLSA based on eight protein-coding genes (gapA, gyrB, ftsZ, mreB, pyrH, recA, rpoA, and topA) showed the vibrios formed an outskirt branch of Halioticoli clade. The experimental DNA-DNA hybridization data revealed that the five strains were in the range of being defined as conspecific but separate from nine Halioticoli clade species. The G+C contents of the Vibrio ishigakensis strains were 47.3-49.1mol%. Both Amino Acid Identity and Average Nucleotide Identity of the strain C1(T) against Vibrio ezurae HDS1-1(T), Vibrio gallicus HT2-1(T), Vibrio halioticoli IAM 14596(T), Vibrio neonatus HDD3-1(T) and Vibrio superstes G3-29(T) showed less than 95% similarity. The genome-based taxonomic approach by means of in silico DDH values also supports the V. ishigakensis strains being distinct from the other known Halioticoli clade species. Sixteen traits (growth temperature range, DNase and lipase production, indole production, and assimilation of 10 carbon compounds) distinguished these strains from Halioticoli clade species. The names V. ishigakensis sp. nov. is proposed for the species of Halioticoli clade, with C1(T) as the type strain (JCM 19231(T)=LMG 28703(T)).
Laboratory of Microbiology, Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Japan.
Feng
F
Gao
Laboratory of Microbiology, Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Japan.
Nurhidayu
N
Al-Saari
Laboratory of Microbiology, Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Japan.
A K M
AK
Rohul Amin
Laboratory of Microbiology, Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Japan.
Kazumichi
K
Sato
Laboratory of Microbiology, Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Japan.
Sayaka
S
Mino
Laboratory of Metagenomics, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Japan; Department of Microbiology and Immunology, Keio University School of Medicine, Tokyo, Japan.
Wataru
W
Suda
Laboratory of Metagenomics, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Japan.
Kenshiro
K
Oshima
Laboratory of Metagenomics, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Japan; Graduate School of Advanced Science and Engineering, Waseda University, Tokyo, Japan.
Masahira
M
Hattori
Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Ibaraki, Japan.
Moriya
M
Ohkuma
Institute of Biology, SAGE-COPPE, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
Paulo Iiboshi
PI
Hargreaves
Institute of Biology, SAGE-COPPE, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
Pedro Milet
PM
Meirelles
Institute of Biology, SAGE-COPPE, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
Fabiano L
FL
Thompson
Institute of Biology, SAGE-COPPE, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
Cristiane
C
Thompson
CIAD, AC Mazatlan Unit for Aquaculture and Environmental Management, AP 711 Mazatlán, Sinaloa, Mexico.
Bruno
B
Gomez-Gil
Department of Food and Nutrition, Hakodate Junior College, Hakodate, Japan.
Toko
T
Sawabe
Laboratory of Microbiology, Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Japan. Electronic address: sawabe@fish.hokudai.ac.jp.
Tomoo
T
Sawabe
D000464:Alginates / Q000378:metabolism; D000818:Animals; D037421:Anthozoa / Q000382:microbiology*; D001482:Base Composition / Q000235:genetics; D001483:Base Sequence; D057894:Coral Reefs; D004269:DNA, Bacterial / Q000235:genetics; D020043:Genes, Essential / Q000235:genetics; D007564:Japan; D058885:Multilocus Sequence Typing; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014733:Vibrio / Q000145:classification / Q000235:genetics / Q000302:isolation & purification
D000464:Alginates; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
Coral reef; Genome taxonomy; Halioticoli clade; Seawater; Vibrio; Vibrionaceae