Multiclass method for pesticides quantification in honey by means of modified QuEChERS and UHPLC-MS/MS.
Bee products can be produced in an environment contaminated by pesticides that can be transported by honey bees to the hive and incorporated into the honey. Therefore, rapid and modern methods to determine pesticide residues in honey samples are essential to guarantee consumers' health. In this study, a simple multiresidue method for the quantification of 116 pesticides in honey is proposed. It involves the use of a modified QuEChERS procedure followed by UHPLC-MS/MS analysis. The method was validated according to the European Union SANCO/12571/2013 guidelines. Acceptable values were obtained for the following parameters: linearity, limit of detection (0.005mg/kg) and limit of quantification (0.010 and 0.025mg/kg), trueness (for the four tested levels the recovery assays values were between 70 and 120%), intermediate precision (RSD<20.0%) and measurement uncertainty tests (<50.0%). The validated method was applied for determination of 100 honey samples from five states of Brazil.
LBqA - Laboratório de Bioquímica de Alimentos, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, bloco 3, sala 2091, Pampulha, CEP 31210-901 Belo Horizonte, MG, Brazil.
Patrícia Amaral Souza
PA
Tette
LANAGRO-MG, Ministério da Agricultura Pecuária e Abastecimento, MAPA, Pedro Leopoldo, MG, Brazil.
Fabiano Aurélio
FA
da Silva Oliveira
LANAGRO-MG, Ministério da Agricultura Pecuária e Abastecimento, MAPA, Pedro Leopoldo, MG, Brazil.
Elba Nathália Corrêa
EN
Pereira
LANAGRO-MG, Ministério da Agricultura Pecuária e Abastecimento, MAPA, Pedro Leopoldo, MG, Brazil.
Gilsara
G
Silva
LBqA - Laboratório de Bioquímica de Alimentos, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, bloco 3, sala 2091, Pampulha, CEP 31210-901 Belo Horizonte, MG, Brazil.
Maria Beatriz
MB
de Abreu Glória
Laboratório de Controle de Qualidade de Medicamentos e Cosméticos, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, bloco 2, sala 4029, Pampulha, CEP 31210-901 Belo Horizonte, MG, Brazil. Electronic address: cfernandes@farmacia.ufmg.br.
Christian
C
Fernandes
D000818:Animals; D001516:Bees; D001938:Brazil; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005062:European Union; D005506:Food Contamination / Q000032:analysis*; D006722:Honey / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
D010573:Pesticide Residues
Honey; Pesticides; Proficiency test; QuEChERS; UHPLC–MS/MS