Prevalence of IDH1/2 Mutations in Different Subtypes of Glioma in the North-East Population of Morocco.
BACKGROUND
Genetic alterations in gliomas have increasing importance for classification purposes. Thus, we are especially interested in studying IDH mutations which may feature potential roles in diagnosis, prognosis and response to treatment. Our aim was to investigate IDH mutations in diffuse glioma patients diagnosed in university hospital centre of Fez in Morocco.


METHODS
IDH1 codon 132 and IDH2 codon 172 were direct-sequenced in 117 diffuse glioma samples diagnosed and treated in University Hospital Hassan II between 2010 and 2014.


RESULTS
The R132H IDH1 mutation was identified in 43/117 tumor samples and R172K IDH2 mutation was detected in only one anaplastic oligodendroglioma. IDH mutations were observed in 63.2% of astrocytomas, 73.3% of diffuse oligodendrogliomas and 12.90% of glioblastomas.


CONCLUSIONS
Our results confirmed other studies published earlier for other populations with some small discrepancies.
Groupe Hospitalier Pitie-Salpetriere, Laboratoire de Neuropathologie R Escourolle, Paris, France E-mail : nadia.senhaji@usmba.ac.ma.
Nadia
N
Senhaji
Sara
S
Louati
Laila
L
Chbani
Sanae El
SE
Bardai
Karima
K
Mikou
Mustafa
M
Maaroufi
Mohammed
M
Benzagmout
Mohammed Chaoui El
MC
Faiz
Yannick
Y
Marie
Karima
K
Mokhtari
Ahmed
A
Idbaih
Afaf
A
Amarti
Sanae
S
Bennis
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D001932:Brain Neoplasms / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology; D002675:Child, Preschool; D005260:Female; D005500:Follow-Up Studies; D005910:Glioma / Q000453:epidemiology / Q000235:genetics* / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D017404:In Situ Hybridization, Fluorescence; D007521:Isocitrate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009018:Morocco / Q000453:epidemiology; D009154:Mutation / Q000235:genetics*; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D015995:Prevalence; D011379:Prognosis; D012189:Retrospective Studies
D014408:Biomarkers, Tumor; C538784:IDH2 protein, human; D007521:Isocitrate Dehydrogenase; C543588:IDH1 protein, human