Fast, sensitive point of care electrochemical molecular system for point mutation and select agent detection.
Point of care molecular diagnostics benefits from a portable battery-operated device capable of performing a fast turnaround using reliable inexpensive cartridges. We describe a prototype device for performing a molecular diagnostics test for clinical and biodefense samples in 16 minutes using a prototype capable of an 8 minute PCR reaction, followed by hybridization and detection on an electrochemical microarray based on the i-STAT® system. We used human buccal swabs for hemochromatosis testing including in-device DNA extraction. Additional clinical and biodefense samples included influenza A and bacterial select agents Bacillus anthracis, Yersinia pestis and Francisella tularensis.
Assay Research, Abbott Point of Care, 185 Corkstown Road, Ottawa, Ontario K2H 8V4, Canada.
J A
JA
MacLeod
A C
AC
Nemeth
W C
WC
Dicke
D
D
Wang
S
S
Manalili Wheeler
J C
JC
Hannis
G B
GB
Collier
J J
JJ
Drader
D001408:Bacillus anthracis / Q000235:genetics; D004252:DNA Mutational Analysis / Q000295:instrumentation / Q000379:methods; D055664:Electrochemical Techniques / Q000295:instrumentation / Q000379:methods*; D005604:Francisella tularensis / Q000235:genetics; D005838:Genotype; D006432:Hemochromatosis / Q000175:diagnosis / Q000235:genetics; D006801:Humans; D009980:Influenza A virus / Q000235:genetics; D056656:Lab-On-A-Chip Devices; D025202:Molecular Diagnostic Techniques / Q000295:instrumentation* / Q000379:methods; D017354:Point Mutation; D019095:Point-of-Care Systems; D016133:Polymerase Chain Reaction; D013997:Time Factors; D015010:Yersinia pestis / Q000235:genetics