Human amniotic fluid derived mesenchymal stem cells cause an anti-cancer effect on breast cancer cell line in vitro.
Human amniotic fluid stem cells (hAFSCs) have the ability to self-renew, and multipotent differentiation into three germ layer cells. We obtained 5 ml amniotic fluid from ten 16-20 week pregnant women undergoing amniocentesis. hAFSCs were isolated from all samples, co-cultured with T47D breast cancer cell line and characterized using flow cytometry and RT-PCR. After 3, 4 and 5 days, T47D and HSFCs viability were evaluated with MTT assay. After 5 days of co-culture T47D cells viability were decreased. Our findings showed that hAFSCs can release soluble factors in cell culture, causing an efficient anticancer effect.
Lorestan University of Medical sciences Assalian Hospital, Center for Obstetrics and Gynecology khoramabad Iran m.ghafarzadeh95@gmail.com.
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Ghafarzadeh
Tehran University of Medical sciences Department of Medical Biotechnology, School of advance Science in Medicine Tehran Iran.
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Eatemadi
Lorestan University of Medical sciences Razi Drug applied research center khoramabad Iran.
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Fakhravar
D000653:Amniotic Fluid / Q000166:cytology*; D000970:Antineoplastic Agents / Q000378:metabolism*; D001943:Breast Neoplasms / Q000473:pathology*; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002469:Cell Separation; D048430:Cell Shape; D002470:Cell Survival; D018920:Coculture Techniques; D015925:Cryopreservation; D018076:DNA, Complementary / Q000096:biosynthesis; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D012313:RNA / Q000302:isolation & purification; D060888:Real-Time Polymerase Chain Reaction
D000970:Antineoplastic Agents; D018076:DNA, Complementary; D012313:RNA