Down-regulated and Commonly mutated ALPK1 in Lung and Colorectal Cancers.
The ALPK1 gene located in the 4q25 region encodes a newly explored protein kinase which could phosphorylate the amino acid of a domain full of α-helices. Recently, several studies have indicated that the expression of ALPK1 is related to inflammation and various diseases; therefore, the purpose of this investigation was to determine whether the expression of ALPK1 has an influence on tumorigenesis and to further scrutinize its gene polymorphism in order to better understand its clinical importance. In lung and colorectal cancer tissues, the ALPK1 RNA level of the normal part was higher than that of the tumor part using the RT-qPCR analysis. Moreover, differences in HRM melting curves could effectively separate the known mutation sites and be used to identify the two novel variants that might cause the bio-dysfunctions of ALPK1 found in silico predictions. Additionally, in both Lovo colorectal and A549 lung cancer cells with enhanced and depleted expression of ALPK1, the encoded ALPK1 could exert its activity on cell migration without interfering with cell viability. Taken together, these findings suggested that ALPK1 might play a vital role in cancer development and that the newly explored SNPs are found in a Taiwanese cohort.
The Ph.D. Program for Cancer Biology and Drug Discovery, China Medical University and Academia Sinica, Taichung, Taiwan.
Hsien-Feng
HF
Liao
Epigenome Research Center, China Medical University Hospital, Taichung, Taiwan.|Department of Laboratory Medicine, China Medical University Hospital, Taichung, Taiwan.
Hsien-Hsiung
HH
Lee
Epigenome Research Center, China Medical University Hospital, Taichung, Taiwan.|Department of Laboratory Medicine, China Medical University Hospital, Taichung, Taiwan.
Ya-Sian
YS
Chang
Epigenome Research Center, China Medical University Hospital, Taichung, Taiwan.
Chia-Li
CL
Lin
Epigenome Research Center, China Medical University Hospital, Taichung, Taiwan.
Ting-Yuan
TY
Liu
Epigenome Research Center, China Medical University Hospital, Taichung, Taiwan.
Yu-Chia
YC
Chen
Epigenome Research Center, China Medical University Hospital, Taichung, Taiwan.
Ju-Chen
JC
Yen
Epigenome Research Center, China Medical University Hospital, Taichung, Taiwan.
Ya-Ting
YT
Lee
Department of Laboratory Medicine, China Medical University Hospital, Taichung, Taiwan.
Chien-Yu
CY
Lin
The Ph.D. Program for Cancer Biology and Drug Discovery, China Medical University and Academia Sinica, Taichung, Taiwan.|Institute of Biological Chemistry, Academia Sinica, Taipei 11529, Taiwan.
Shih-Hsiung
SH
Wu
Environment-Omics-Disease Research Centre, China Medical University Hospital, Taichung, Taiwan.|Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan.
Ying-Chin
YC
Ko
The Ph.D. Program for Cancer Biology and Drug Discovery, China Medical University and Academia Sinica, Taichung, Taiwan.|Epigenome Research Center, China Medical University Hospital, Taichung, Taiwan.|Department of Laboratory Medicine, China Medical University Hospital, Taichung, Taiwan.|School of Medicine, China Medical University, Taichung, Taiwan.
Jan-Gowth
JG
Chang
D000072283:A549 Cells; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D002470:Cell Survival / Q000235:genetics; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D015536:Down-Regulation / Q000235:genetics; D055785:Gene Knockdown Techniques; D006801:Humans; D007249:Inflammation / Q000473:pathology; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D020641:Polymorphism, Single Nucleotide; D011494:Protein Kinases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D013624:Taiwan
D012333:RNA, Messenger; D011494:Protein Kinases; C000626799:ALPK1 protein, human