2.3THz radiation: Absence of genotoxicity/mutagenicity in Escherichia coli and Salmonella typhimurium.
The mutagenicity and genotoxicity in bacteria of 2.3THz radiation (THz) produced by a free-electron laser (NovoFEL) were evaluated; exposures were 5, 10, or 15min at average power 1.4W/cm(2). Two Ames mutagenicity test strains of Salmonella typhimurium, TA98 and TA102, were used. For the genotoxicity test, we measured SOS induction in Escherichia coli PQ37. No significant differences were found between exposed and control cells, indicating that THz radiation is neither mutagenic nor genotoxic under these conditions. Nevertheless, a small increase in total cell number of S. typhimurium after 15min exposure, and an increase in β-galactosidase and alkaline phosphatase activities in E.coli PQ37, were observed, indicating some effect of THz radiation on cell metabolism. We also examined the combined effect of 4-NQO (8μM; positive control) and THz exposure (5min) on genotoxicity in E.coli PQ37. Unexpectedly, THz radiation decreased 4-NQO genotoxicity.
Institute of Cytology and Genetics RAS, Prospekt Lavrentyeva 10, Novosibirsk, 630090, Russia. Electronic address: sergeeva-sv@bionet.nsc.ru.
Svetlana
S
Sergeeva
Institute of Cytology and Genetics RAS, Prospekt Lavrentyeva 10, Novosibirsk, 630090, Russia.
Elisaveta
E
Demidova
Institute of Cytology and Genetics RAS, Prospekt Lavrentyeva 10, Novosibirsk, 630090, Russia.
Olga
O
Sinitsyna
Institute of Cytology and Genetics RAS, Prospekt Lavrentyeva 10, Novosibirsk, 630090, Russia.
Tatiana
T
Goryachkovskaya
Institute of Cytology and Genetics RAS, Prospekt Lavrentyeva 10, Novosibirsk, 630090, Russia.
Alla
A
Bryanskaya
Budker Institute of Nucleic Physics SB RAS, Prospekt Lavrentyeva 11, Novosibirsk, 630090, Russia.
Artem
A
Semenov
Institute of Cytology and Genetics RAS, Prospekt Lavrentyeva 10, Novosibirsk, 630090, Russia.
Irina
I
Meshcheryakova
Oxford Institute for Radiation Oncology, University of Oxford, Department of Oncology, Roosevelt Drive, OX37DQ Oxford, UK.
Grigory
G
Dianov
Budker Institute of Nucleic Physics SB RAS, Prospekt Lavrentyeva 11, Novosibirsk, 630090, Russia.
Vasiliy
V
Popik
Institute of Cytology and Genetics RAS, Prospekt Lavrentyeva 10, Novosibirsk, 630090, Russia.
Sergey
S
Peltek
D004249:DNA Damage / Q000528:radiation effects; D004926:Escherichia coli / Q000378:metabolism / Q000528:radiation effects*; D009152:Mutagenicity Tests; D011485:Protein Binding / Q000528:radiation effects; D012486:Salmonella typhimurium / Q000378:metabolism / Q000528:radiation effects*; D001616:beta-Galactosidase / Q000378:metabolism
D001616:beta-Galactosidase
Ames test; Free electron laser; SOS chromotest; Terahertz radiation