Differential associations of Borrelia species with European badgers (Meles meles) and raccoon dogs (Nyctereutes procyonoides) in western Poland.
European badgers and raccoon dogs and their associated ticks and lice were assayed for the presence of Lyme borreliosis and relapsing fever-group spirochete DNA in western Poland. Analyses of blood, ear-biopsy and liver samples revealed that 25% of 28 raccoon dogs and 12% of 34 badgers were PCR positive for borreliae. Borrelia garinii was the dominant species in raccoon dogs (62.5%), followed by B. afzelii (25%) and B. valaisiana (12.5%). PCR-positive badgers were infected only with B. afzelii. A total of 351 attached ticks was recovered from 23 (82%) of the raccoon dogs and 13 (38%) of the badgers. Using a nested PCR targeting the ITS2 fragments of Ixodes DNA, four Ixodes species were identified: I. ricinus, I. canisuga, I. hexagonus, and one provisionally named I. cf. kaiseri. Ixodes canisuga and I. ricinus prevailed on both host species. The highest infection prevalence was detected in I. ricinus, followed by I. canisuga and I. cf. kaiseri. Borrelia garinii and B. afzelii accounted for 61.6% and 30.1% of the infections detected in all PCR-positive ticks, respectively. Four other Borrelia species (B. burgdorferi sensu stricto, B. valaisiana, B. lusitaniae and B. miyamotoi) were detected only in I. ricinus from raccoon dogs. Moreover, Borrelia DNA, mostly B. garinii, was detected in 57 (81.4%) of 70 Trichodectes melis lice derived from 12 badgers. The detection of B. afzelii in one-half of PCR-positive biopsies reconfirms previous associations of this species with mammalian hosts, whereas the high prevalence of B. garinii in feeding lice and I. ricinus ticks (including larvae) demonstrates that both carnivores serve as hosts for B. garinii. The lack of B. garinii DNA in the tissues of badgers versus its prevalence in raccoon-dog biopsies, however, incriminates only the latter carnivore as a potential reservoir host.
Department of Genetics, Faculty of Biology, Szczecin University, Szczecin, Poland. Electronic address: wodecka.us@gmail.com.
Beata
B
Wodecka
Department of Animal Morphology, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland.
Jerzy
J
Michalik
Department of Environmental Science, Policy and Management, University of California, Berkeley, CA 94720, USA.
Robert S
RS
Lane
Department of Invertebrate Zoology and Parasitology, Institute of Biology, Pedagogical University of Cracow, Kraków, Poland.
Magdalena
M
Nowak-Chmura
Department of Game Management and Forest Protection, Faculty of Forestry, Poznań University of Life Sciences, Poznań, Poland.
Anna
A
Wierzbicka
D000818:Animals; D001706:Biopsy; D001898:Borrelia / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics; D004197:Disease Reservoirs; D004423:Ear / Q000382:microbiology / Q000473:pathology; D018884:Ixodes / Q000382:microbiology; D007814:Larva / Q000382:microbiology; D008099:Liver / Q000382:microbiology; D008193:Lyme Disease / Q000097:blood / Q000453:epidemiology / Q000662:veterinary*; D045969:Mustelidae / Q000382:microbiology*; D008003:Phthiraptera / Q000382:microbiology; D011044:Poland / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D015995:Prevalence; D046031:Raccoon Dogs / Q000382:microbiology*
D004269:DNA, Bacterial
Borrelia; European badgers; Ixodes; Raccoon dogs; Reservoir hosts