High-Efficiency Capture of Individual and Cluster of Circulating Tumor Cells by a Microchip Embedded with Three-Dimensional Poly(dimethylsiloxane) Scaffold.
Effective isolation of circulating tumor cells (CTCs) has great significance for cancer research but is highly challenged. Here, we developed a microchip embedded with a three-dimensional (3D) PDMS scaffold by a quadratic-sacrificing template method for high-efficiency capture of CTCs. The microchip was gifted with a 3D interconnected macroporous structure, strong toughness, and excellent flexibility and transparency, enabling fast isolation and convenient observation of CTCs. Especially, 3D scaffold chip perfectly integrates the two main strategies currently used for enhancement of cell capture efficiency. Spatially distributed 3D scaffold compels cells undergoing chaotic or vortex migration in the channel, and the spatially distributed nanorough skeleton offers ample binding sites, which synergistically and significantly improve CTCs capture efficiency. Our results showed that 1-118 CTCs/mL were identified from 14 cancer patients' blood and 5 out of these cancer patients showed 1-14 CTC clusters/mL. This work demonstrates for the first time the development of microchip with transparent interconnected 3D scaffold for isolation of CTCs and CTC clusters, which may promote in-depth analysis of CTCs.
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University , Wuhan 430072, China.
Shi-Bo
SB
Cheng
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University , Wuhan 430072, China.
Min
M
Xie
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University , Wuhan 430072, China.
Jia-Quan
JQ
Xu
Renmin Hospital of Wuhan University , Wuhan 430060, China.
Jing
J
Wang
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University , Wuhan 430072, China.
Song-Wei
SW
Lv
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University , Wuhan 430072, China.
Shan
S
Guo
Renmin Hospital of Wuhan University , Wuhan 430060, China.
Ying
Y
Shu
Renmin Hospital of Wuhan University , Wuhan 430060, China.
Ming
M
Wang
Renmin Hospital of Wuhan University , Wuhan 430060, China.
Wei-Guo
WG
Dong
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University , Wuhan 430072, China.
Wei-Hua
WH
Huang
D055910:Antibodies, Immobilized / Q000737:chemistry / Q000276:immunology; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D000071858:Epithelial Cell Adhesion Molecule / Q000276:immunology / Q000378:metabolism; D006801:Humans; D061986:MCF-7 Cells; D046228:Microarray Analysis; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D008856:Microscopy, Fluorescence; D009369:Neoplasms / Q000097:blood / Q000473:pathology; D009360:Neoplastic Cells, Circulating / Q000378:metabolism* / Q000473:pathology; D016062:Porosity
D055910:Antibodies, Immobilized; D004129:Dimethylpolysiloxanes; D000071858:Epithelial Cell Adhesion Molecule; C013830:baysilon