Campylobacter geochelonis sp. nov. isolated from the western Hermann's tortoise (Testudo hermanni hermanni).
During a screening study to determine the presence of species of the genus Campylobacter in reptiles, three putative strains (RC7, RC11 and RC20T) were isolated from different individuals of the western Hermann's tortoise (Testudo hermanni hermanni). Initially, these isolates were characterized as representing Campylobacterfetus subsp. fetus by multiplex PCR and partial 16S rRNA gene sequence analysis. Further whole- genome characterization revealed considerable differences compared to other Campylobacter species. A polyphasic study was then undertaken to determine the exact taxonomic position of the isolates. The three strains were characterized by conventional phenotypic tests and whole genome sequencing. We generated robust phylogenies that showed a distinct clade containing only these strains using the 16S rRNA and atpA genes and a set of 40 universal proteins. Our phylogenetic analysis demonstrates their designation as representing a novel species and this was further confirmed using whole- genome average nucleotide identity within the genus Campylobacter (~80 %). Compared to most Campylobacter species, these strains hydrolysed hippurate, and grew well at 25 °C but not at 42 °C. Phenotypic and genetic analyses demonstrate that the three Campylobacter strains isolated from the western Hermann's tortoise represent a novel species within the genus Campylobacter, for which the name Campylobactergeochelonis sp. nov. is proposed, with RC20T (=DSM 102159T=LMG 29375T) as the type strain.
Department of Comparative Biomedicine and Food Science, University of Padua, Padua, Italy.
Alessandra
A
Piccirillo
Department of Comparative Biomedicine and Food Science, University of Padua, Padua, Italy.
Giulia
G
Niero
Sección Genética Evolutiva, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay.
Lucía
L
Calleros
Sección Genética Evolutiva, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay.
Ruben
R
Pérez
Unidad de Bioinformática, Institut Pasteur Montevideo, Uruguay.|Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay.
Hugo
H
Naya
Sección Genética Evolutiva, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay.|Unidad de Bioinformática, Institut Pasteur Montevideo, Uruguay.
Gregorio
G
Iraola
D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002167:Campylobacter / Q000145:classification* / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014426:Turtles / Q000382:microbiology*
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S