Fabrication of Electrochemical Model Influenza A Virus Biosensor Based on the Measurements of Neuroaminidase Enzyme Activity.
Neuroaminidase (NA) enzyme is a kind of glycoprotein that is found on the influenza A virus. During infection, NA is important for the release of influenza virions from the host cell surface together with viral aggregates. It may also be involved in targeting the virus to respiratory epithelial cells. In this study, a model electrochemical influenza A viral biosensor in which receptor-binding properties have been based on NA was developed for the first time. The biosensor's working principle is based on monitoring the interactions between fetuin A and NA enzyme. The assay was monitored step by step by using electrochemical impedance spectroscopy.
Mugla Sitki Kocman University , Faculty of Science, Chemistry Department, Kotekli, Mugla, Turkey 48000.
Ülkü
Ü
Anik
Mugla Sitki Kocman University , Faculty of Science, Chemistry Department, Kotekli, Mugla, Turkey 48000.
Yudum
Y
Tepeli
Institut Pasteur de Tunis, Université Tunis Elmanar , LR11 IPT03, PB74, 13 Place Pasteur, Le Belvédère, 1002 Tunis, Tunisia.|Faculté des Sciences de Bizerte, Université de Carthage , Tunisia.
Mohamed F
MF
Diouani
D015374:Biosensing Techniques; D058266:Dielectric Spectroscopy; D004566:Electrodes; D009980:Influenza A virus / Q000201:enzymology*; D008954:Models, Biological; D009439:Neuraminidase / Q000378:metabolism*; D019887:Peanut Agglutinin / Q000378:metabolism; D011485:Protein Binding; D060749:alpha-2-HS-Glycoprotein / Q000378:metabolism
D019887:Peanut Agglutinin; D060749:alpha-2-HS-Glycoprotein; D009439:Neuraminidase