Knockdown of eIF3a attenuates the pro-fibrogenic response of hepatic stellate cells induced by TGF-β1.
Activation of hepatic stellate cells (HSCs) plays an important role in the development of liver fibrosis. The eukaryotic translation initiation factor (eIF) 3a is the largest subunit of the eIF3 complex and has been involved in pulmonary fibrosis. However, the role of eIF3a in liver fibrosis remains largely unknown. Therefore, in this study, we investigated the role of eIF3a in transforming growth factor-β1 (TGF-β1)-induced HSC activation. Our results demonstrated that the expression of eIF3a was up-regulated in human liver fibrotic tissues and activated HSCs. In addition, knockdown of eIF3a suppressed TGF-β-induced HSC proliferation and the expression of α-smooth muscle actin (α-SMA) and collagen I. Furthermore, knockdown of eIF3a inhibited the expression of p-Smad3 induced by TGF-β1 in HSCs. These results suggest that eIF3a may function as a novel regulator to modulate HSC activation, potentially through inhibiting the TGF-β1/Smad3 signaling pathway.
The First Affiliated Hospital of Zhengzhou University Department of Infectious Diseases Zhengzhou China.
P
P
He
The First Affiliated Hospital of Zhengzhou University Department of Infectious Diseases Zhengzhou China.
Z-J
ZJ
Yu
The First Affiliated Hospital of Zhengzhou University Department of Infectious Diseases Zhengzhou China scyuinfec@163.com.
C-Y
CY
Sun
The First Affiliated Hospital of Zhengzhou University Department of Neurology Zhengzhou China.
S-J
SJ
Jiao
The First Affiliated Hospital of Zhengzhou University Department of Infectious Diseases Zhengzhou China.
H-Q
HQ
Jiang
D000199:Actins / Q000378:metabolism; D049109:Cell Proliferation; D024042:Collagen Type I / Q000378:metabolism; D039621:Eukaryotic Initiation Factor-3 / Q000378:metabolism*; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D055166:Hepatic Stellate Cells / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D008103:Liver Cirrhosis / Q000378:metabolism* / Q000473:pathology*; D010766:Phosphorylation; D015398:Signal Transduction; D051900:Smad3 Protein / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
C541116:ACTA2 protein, human; D000199:Actins; D024042:Collagen Type I; D039621:Eukaryotic Initiation Factor-3; D051900:Smad3 Protein; D053773:Transforming Growth Factor beta1