Pseudogracilibacillus marinus sp. nov., isolated from a biofilm formed in coastal seawater.
A Gram-staining-positive, aerobic, motile, rod-shaped (0.4-0.5×2.0-4.0 µm), endospore-forming bacterium, designated strain NIOT.bflm.S4T, was isolated from biofilm formed on high-density polyethylene test coupons in coastal seawater. The strain required seawater for growth. It grew with 1.0-8.0 % (w/v) NaCl, at 4-45 °C and at pH 6.5-9.0, with optimum growth with 4.0-5.0 % (w/v) NaCl, at 30 °C and at pH 7.0-8.0. Phylogenetic analyses based on 16S rRNA and partial dnaK gene sequences showed that strain NIOT.bflm.S4T formed a phylogenetic lineage with Pseudogracilibacillus auburnensis P-207T, the only known species of the genus Pseudogracilibacillusand shared sequence identities of 96.9 and 83 %, respectively, with this strain. The identities of 16S rRNA and partial dnaK gene sequences with members of other related genera such as Gracilibacillus, Paraliobacillus, Ornithinibacillus, Oceanobacillus, Virgibacillus and Lentibacillus were ≤95 and ≤78 %, respectively. The DNA G+C content of strain NIOT.bflm.S4T was 39.1 mol%. MK-7 was found as the sole isoprenoid quinone. The major polar lipids of strain NIOT.bflm.S4T were diphosphatidylglycerol, phosphatidylethanolamine and an unknown lipid. The diagnostic diamino acid of the cell-wall peptidoglycan was meso-diaminopimelic acid. Major cellular fatty acids were anteiso-C15 : 0 (27.9 %), anteiso-C17 : 0 (18.6 %), C12 : 0 (8.7 %) and iso-C15 : 0 (6.6 %). On the basis of phenotypic, phylogenetic and chemotaxonomic results, we propose that the isolate represents a novel species of the genus Pseudogracilibacillus, for which the name Pseudogracilibacillus marinus sp. nov. is proposed. The type strain is NIOT.bflm.S4T (=KACC 18456T=MTCC 12376T=TBRC 5831T).
Marine Biotechnology Division, OSTI Group, National Institute of Ocean Technology, Ministry of Earth Sciences (MoES), Government of India, Chennai 600100, Tamil Nadu, India.
Pankaj
P
Verma
Marine Biotechnology Division, OSTI Group, National Institute of Ocean Technology, Ministry of Earth Sciences (MoES), Government of India, Chennai 600100, Tamil Nadu, India.
Narasingaperumal
N
Saravanan
Department of Life Science & Bio-Integration Research Center for Nutra-Pharmaceutical Epigenetics, Chung-Ang University, Seoul 06974, Republic of Korea.
Baolei
B
Jia
Department of Life Science & Bio-Integration Research Center for Nutra-Pharmaceutical Epigenetics, Chung-Ang University, Seoul 06974, Republic of Korea.
Che Ok
CO
Jeon
Marine Biotechnology Division, OSTI Group, National Institute of Ocean Technology, Ministry of Earth Sciences (MoES), Government of India, Chennai 600100, Tamil Nadu, India.
Gopal
G
Dharani
CAS-Marine Biology, Annamalai University, Parangipettai 608502, Tamil Nadu, India.
S T
ST
Somasundaram
Marine Biotechnology Division, OSTI Group, National Institute of Ocean Technology, Ministry of Earth Sciences (MoES), Government of India, Chennai 600100, Tamil Nadu, India.
Ramalingam
R
Kirubagaran
D001406:Bacillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D018441:Biofilms; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D005798:Genes, Bacterial; D007194:India; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D003960:Diaminopimelic Acid