Using Fluorescence Imaging to Track Drug Delivery and Guide Treatment Planning In Vivo.
Imaging has become an indispensable tool in both clinical medicine and preclinical sciences. It enables doctors to locate sites of cancer/disease, track drug delivery, and guide operative planning, thus enhancing the treatment efficacy. Recently, we developed a multimodal theranostic lipid nanoparticles, named HPPS(NIR)-chol-siRNA with its built-in near-infrared (NIR) fluorescent probe core as a useful surrogate for tracking small interfering RNA (siRNA) delivery. By using the image co-registration of computed tomography (CT) and fluorescence molecular tomography (FMT), we achieved noninvasive assessment and treatment planning of siRNA delivery into the orthotopic tumor, thus enabling efficacious RNA interference (RNAi) therapy. In this chapter, we introduce this method to illustrate the use of CT-FMT co-registration for tracking drug delivery and guiding treatment planning in vivo.
Princess Margaret Cancer Center, University Health Network, Toronto, ON, Canada.|Department of Medical Biophysics, University of Toronto, Toronto, ON, Canada.|Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan, China.
Qiaoya
Q
Lin
Princess Margaret Cancer Center, University Health Network, Toronto, ON, Canada.|DLVR Therapeutics Inc., Toronto, ON, Canada.
Huang
H
Huang
Princess Margaret Cancer Center, University Health Network, Toronto, ON, Canada. juanchen@uhnresearch.ca.
Juan
J
Chen
Princess Margaret Cancer Center, University Health Network, Toronto, ON, Canada. gang.zheng@uhnres.utoronto.ca.|Department of Medical Biophysics, University of Toronto, Toronto, ON, Canada. gang.zheng@uhnres.utoronto.ca.
Gang
G
Zheng
D000818:Animals; D045744:Cell Line, Tumor; D016903:Drug Monitoring / Q000379:methods; D006801:Humans; D008297:Male; D051379:Mice; D064847:Multimodal Imaging; D053758:Nanoparticles; D011471:Prostatic Neoplasms / Q000000981:diagnostic imaging* / Q000188:drug therapy*; D034741:RNA, Small Interfering / Q000008:administration & dosage*; D041622:Tomography, Optical / Q000379:methods*; D014057:Tomography, X-Ray Computed; D023041:Xenograft Model Antitumor Assays
D034741:RNA, Small Interfering
Computed tomography; Fluorescence imaging; Fluorescence molecular tomography; HDL; Nanoparticles; siRNA delivery