Fusarium proliferatum strains change fumonisin biosynthesis and accumulation when exposed to host plant extracts.
Fumonisin concentrations in mycelia and media were studied in liquid Fusarium proliferatum cultures supplemented with host plant extracts. Furthermore, the kinetics of fumonisin accumulation in media and mycelia collected before and after extract addition was analysed as well as the changes in the expression of the FUM1 gene. Fumonisin content in culture media increased in almost all F. proliferatum strains shortly after plant extracts were added. The asparagus extract induced the highest FB level increase and the garlic extract was the second most effective inducer. Fumonisin level decreased constantly until 14th day of culturing, though for some strains also at day 8th an elevated FB level was observed. Pineapple extract induced the highest increase of fum1 transcript levels as well as fumonisin synthesis in many strains, and the peas extract inhibited fungal growth and fumonisin biosynthesis. Moreover, fumonisins were accumulated in mycelia of studied strains and in the respective media.
Department of Pathogen Genetics and Plant Resistance, Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, Poland.
Karolina
K
Górna
Department of Environmental Stress Biology, Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, Poland.
Izabela
I
Pawłowicz
Department of Chemistry, Poznań University of Life Sciences, Wojska Polskiego 75, 60-625 Poznań, Poland.
Agnieszka
A
Waśkiewicz
Department of Pathogen Genetics and Plant Resistance, Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, Poland. Electronic address: lste@igr.poznan.pl.
Łukasz
Ł
Stępień
D031230:Ananas / Q000737:chemistry; D000070357:Asparagaceae / Q000737:chemistry; D053898:Biosynthetic Pathways / Q000235:genetics; D003470:Culture Media / Q000737:chemistry; D037341:Fumonisins / Q000378:metabolism*; D005670:Fusarium / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism*; D005737:Garlic / Q000737:chemistry; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D025282:Mycelium / Q000737:chemistry; D018532:Pisum sativum / Q000737:chemistry; D010936:Plant Extracts / Q000378:metabolism*
D003470:Culture Media; D037341:Fumonisins; D010936:Plant Extracts
Fumonisins; Gene expression; Mycotoxin biosynthetic genes; Plant–pathogen interaction; Secondary metabolites