Chemical composition and anti-inflammatory activity of the leaves of Byrsonima verbascifolia.
An ethnopharmacological survey indicates that the genus Byrsonima has some medicinal species that are commonly found in the Brazilian Cerrado and has been used as an anti-inflammatory and for gastroduodenal disorders. The aim of this study was to evaluate the anti-inflammatory and antioxidant activity along with qualitative chemical characterization of the methanolic extract of the leaves of Byrsonima verbascifolia (BvME) obtained by exhaustive percolation. The data from the chemical analyses by liquid chromatography-mass spectrometry led to tentative identification of 42 compounds belonging to proanthocyanidins, galloyl quinic acid derivatives, flavonoids, and triterpene glycoside derivatives. BvME contain flavonoids and show an antioxidative activity. The methanolic extract administered intraperitoneally at doses of 50, 100, or 300 mg/kg showed a significant reduction in paw edema and modulated the neutrophil influx in a mouse model. Furthermore, the anti-edematogenic activity of the extract provided in smaller doses (12.5 and 25 mg/kg) was also demonstrated in a mouse paw edema model. The extract inhibited NO production by macrophages induced by lipopolysaccharide. We presume that the anti-inflammatory effects of BvME are due to a combination of compounds present in B. verbascifolia, including catechins (procyanidins), flavonoids, and triterpene glycosides and that these anti-inflammatory actions should be mediated, at least partly, through the inhibition of NO production. This study supports and validates the ethnopharmacological uses of B. verbascifolia as an anti-inflammatory.
Campus Centro-Oeste Dona Lindu, UFSJ, Sebastião Gonçalves Coelho, 400, Chanadour, Divinópolis, MG, 35501-296, Brazil.
Aline Aparecida
AA
Saldanha
Campus Centro-Oeste Dona Lindu, UFSJ, Sebastião Gonçalves Coelho, 400, Chanadour, Divinópolis, MG, 35501-296, Brazil.
Lucas Fernandes
LF
do Carmo
Campus Centro-Oeste Dona Lindu, UFSJ, Sebastião Gonçalves Coelho, 400, Chanadour, Divinópolis, MG, 35501-296, Brazil.
Sara Batista
SB
do Nascimento
Departamento de Farmacologia, UFMG, Belo Horizonte, MG, 31270-100, Brazil.
Natália Alves
NA
de Matos
Departamento de Farmacologia, UFMG, Belo Horizonte, MG, 31270-100, Brazil.
Clarice
C
de Carvalho Veloso
Campus Centro-Oeste Dona Lindu, UFSJ, Sebastião Gonçalves Coelho, 400, Chanadour, Divinópolis, MG, 35501-296, Brazil.
Ana Hortência Fonsêca
AH
Castro
BU Bioscience, Plant Research International, POB 16, 6700 AA, Wageningen, The Netherlands.|Centre for Biosystems Genomics, POB 98, 6708 PB, Wageningen, The Netherlands.|Netherlands Metabolomics Centre, Einsteinweg 55, 2333 CC, Leiden, The Netherlands.
Ric C H
RC
De Vos
Departamento de Farmacologia, UFMG, Belo Horizonte, MG, 31270-100, Brazil.
André
A
Klein
Campus Centro-Oeste Dona Lindu, UFSJ, Sebastião Gonçalves Coelho, 400, Chanadour, Divinópolis, MG, 35501-296, Brazil.
João Máximo
JM
de Siqueira
BU Bioscience, Plant Research International, POB 16, 6700 AA, Wageningen, The Netherlands.|Centro de Ciências Biológicas e da Saúde, UFMS, Campo Grande, MS, 79070-900, Brazil.
Carlos Alexandre
CA
Carollo
Centro de Ciências Biológicas e da Saúde, UFMS, Campo Grande, MS, 79070-900, Brazil.
Thalita Vieira
TV
do Nascimento
Centro de Ciências Biológicas e da Saúde, UFMS, Campo Grande, MS, 79070-900, Brazil.
Mônica Cristina
MC
Toffoli-Kadri
Campus Centro-Oeste Dona Lindu, UFSJ, Sebastião Gonçalves Coelho, 400, Chanadour, Divinópolis, MG, 35501-296, Brazil. adrianasouza@ufsj.edu.br.
Adriana Cristina
AC
Soares
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001938:Brazil; D002392:Catechin / Q000494:pharmacology / Q000627:therapeutic use; D004195:Disease Models, Animal; D004487:Edema; D005260:Female; D005419:Flavonoids / Q000494:pharmacology / Q000627:therapeutic use; D006027:Glycosides / Q000494:pharmacology / Q000627:therapeutic use; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D008070:Lipopolysaccharides; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008297:Male; D029581:Malpighiaceae / Q000737:chemistry*; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D009569:Nitric Oxide / Q000096:biosynthesis*; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D018515:Plant Leaves / Q000737:chemistry; D014315:Triterpenes / Q000494:pharmacology / Q000627:therapeutic use
D000893:Anti-Inflammatory Agents; D005419:Flavonoids; D006027:Glycosides; D008070:Lipopolysaccharides; D010936:Plant Extracts; D014315:Triterpenes; D009569:Nitric Oxide; D002392:Catechin
Anti-inflammation; Byrsonima verbascifolia leaves; Flavonoids; Mass spectrometry; Nitric oxide