Matrine suppresses airway inflammation by downregulating SOCS3 expression via inhibition of NF-κB signaling in airway epithelial cells and asthmatic mice.
Matrine has been demonstrated to attenuate allergic airway inflammation. Elevated suppressor of cytokine signaling 3 (SOCS3) was correlated with the severity of asthma. The aim of this study was to investigate the effect of matrine on SOCS3 expression in airway inflammation. In this study, we found that matrine significantly inhibited OVA-induced AHR, inflammatory cell infiltration, goblet cell differentiation, and mucous production in a dose-dependent manner in mice. Matrine also abrogated the level of interleukin (IL)-4 and IL-13, but enhanced interferon (IFN)-γ expression, both in BALF and in lung homogenates. Furthermore, matrine impeded TNF-α-induced the expression of IL-6 and adhesion molecules in airway epithelial cells (BEAS-2B and MLE-12). Additionally, we found that matrine inhibited SOCS3 expression, both in asthmatic mice and TNF-α-stimulated epithelial cells via suppression of the NF-κB signaling pathway by using pcDNA3.1-SOCS3 plasmid, SOCS3 siRNA, or nuclear factor kappa-B (NF-κB) inhibitor PDTC.



Matrine suppresses airway inflammation by downregulating SOCS3 expression via inhibition of NF-κB signaling in airway epithelial cells and asthmatic mice.
Department of Respiration, Xi'an Children's Hospital, Xi'an 710003, PR China.
Daqing
D
Sun
Department of Neonatology, Xi'an Children's Hospital, Xi'an 710003, PR China.
Jing
J
Wang
Outpatient Department, School of Aerospace Engineering, Air Force Engineering University, Xi'an 710038, PR China.
Niandi
N
Yang
Department of Quality Control, Xi'an Children's Hospital, Xi'an 710003, PR China. Electronic address: drhaixinma@163.com.
Haixin
H
Ma
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000378:metabolism*; D001991:Bronchitis / Q000378:metabolism / Q000517:prevention & control*; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D016328:NF-kappa B / Q000378:metabolism*; D010047:Ovalbumin / Q000633:toxicity; D011807:Quinolizines / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects*; D000071223:Suppressor of Cytokine Signaling 3 Protein / Q000235:genetics / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000633:toxicity; D000093842:Matrines
D000470:Alkaloids; D016328:NF-kappa B; D011807:Quinolizines; C106644:SOCS3 protein, human; D000071223:Suppressor of Cytokine Signaling 3 Protein; D014409:Tumor Necrosis Factor-alpha; D010047:Ovalbumin; D000093842:Matrines
Asthma; Matrine; NF-κB; SOCS3