Indirect coculture of stem cells with fetal chondrons using PCL electrospun nanofiber scaffolds.
In vitro coculture system provides a powerful tool for tissue engineering. In this study, we evaluated the gene expressions of human adipose-derived stem cells (ASCs) on polycaprolactone (PCL) scaffold in coculture model with fetal chondrons. Electrospun PCL scaffolds (900 nm fiber diameter) were created and human infrapatellar fat pad-adipose-derived stem cells (IPFP-ASCs) were seeded on these scaffolds. Scanning electron microscopy (SEM) showed attachment of human IPFP-ASCs to scaffold. IPFP-ASCs on scaffolds were cocultured with fetal chondrons in transwell. Gene expressions were investigated using real-time polymerase chain reaction (real-time PCR). In comparison with control group, the expression level of collagen type 2 and aggrecan were significantly decreased but Indian Hedgehog(IHH) significantly increased (P < 0.05).These findings may interpreted that IPFP-ASCs seeded on PCL scaffold, in cocultures with fetal chondrons are tending toward osteogenesis rather than chondrogenesis.
a Department of Anatomical Sciences, Faculty of Medicine , Tabriz University of Medical Sciences , Tabriz , Iran.
Parisa
P
Nikpou
a Department of Anatomical Sciences, Faculty of Medicine , Tabriz University of Medical Sciences , Tabriz , Iran.|b Umblical Cord Stem Cell Research Center , Tabriz University of Medical Sciences , Tabriz , Iran.
Jafar
J
Soleimani Rad
c Drug Applied Research Center , Tabriz University of Medical Sciences , Tabriz , Iran.
Daryoush
D
Mohammad Nejad
d Department of Biochemistry and Laboratory Medicine, Faculty of Advanced Medical Sciences , Tabriz University of Medical Sciences , Tabriz , Iran.
Nasser
N
Samadi
a Department of Anatomical Sciences, Faculty of Medicine , Tabriz University of Medical Sciences , Tabriz , Iran.
Leila
L
Roshangar
e Department of Orthopedics , Tabriz University of Medical Sciences , Tabriz , Iran.
Amir Mohammad
AM
Navali
a Department of Anatomical Sciences, Faculty of Medicine , Tabriz University of Medical Sciences , Tabriz , Iran.
Hajar
H
Shafaei
a Department of Anatomical Sciences, Faculty of Medicine , Tabriz University of Medical Sciences , Tabriz , Iran.
Hojjatollah
H
Nozad Charoudeh
f Department of Gynecology & Obstetrics, Faculty of Medicine , Tabriz University of Medical Sciences , Tabriz , Iran.
Neda
N
Danandeh Oskoei
f Department of Gynecology & Obstetrics, Faculty of Medicine , Tabriz University of Medical Sciences , Tabriz , Iran.
Sara
S
Soleimani Rad
D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D000328:Adult; D018920:Coculture Techniques; D024043:Collagen Type II / Q000096:biosynthesis; D005260:Female; D005333:Fetus / Q000166:cytology / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D057139:Nanofibers / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D054457:Tissue Scaffolds / Q000737:chemistry*
D024043:Collagen Type II; D011091:Polyesters; C016240:polycaprolactone
Coculture; fetal chondrons; gene expression; infrapatellar fat pad; poly(ɛ-caprolactone)