Molecular Characterization of Two Fatty Acyl-CoA Reductase Genes From Phenacoccus solenopsis (Hemiptera: Pseudococcidae).
Fatty acyl-CoA reductases (FARs) are key enzymes involved in fatty alcohol synthesis. Here, we cloned and characterized full-length cDNAs of two FAR genes from the cotton mealybug, Phenacoccus solenopsis. The results showed PsFAR I and PsFAR II cDNAs were 1,584 bp and 1,515 bp in length respectively. Both PsFAR I and PsFAR II were predicted to be located in the endoplasmic reticulum by Euk-mPLoc 2.0 approach. Both of them had a Rossmann folding region and a FAR_C region. Two conservative motifs were discovered in Rossmann folding region by sequence alignment including a NADPH combining motif, TGXXGG, and an active site motif, YXXXK. A phylogenetic tree made using MEGA 6.06 indicated that PsFAR I and PsFAR II were placed in two different branches. Gene expression analysis performed at different developmental stages showed that the expression of PsFar I is significantly higher than that of PsFar II in first and second instar nymphs and in male adults. Spirotetramat treatment at 125 mg/liter significantly increased the expression of PsFar I in third instar nymphs, but there was no effect in the expression of PsFar II Our results indicated these two FAR genes showed different expression patterns during insect development and after pesticide treatment, suggesting they play different roles in insect development and detoxification against pesticides.
School of Forestry and Biotechnology, Zhejiang A&F University, Linan, Zhejiang, China (190226808@qq.com; 490731301@qq.com; zhangdayu@zafu.edu.cn) Collaborative Innovation Center of Green Pesticide, Zhejiang Province, Zhejiang A&F University, Linan, Zhejiang, China (190226808@qq.com; 490731301@qq.com; 554669561@qq.com;hnahaq@163.com; zhangdayu@zafu.edu.cn).
Xiaolong
X
Li
School of Forestry and Biotechnology, Zhejiang A&F University, Linan, Zhejiang, China (190226808@qq.com; 490731301@qq.com; zhangdayu@zafu.edu.cn) Collaborative Innovation Center of Green Pesticide, Zhejiang Province, Zhejiang A&F University, Linan, Zhejiang, China (190226808@qq.com; 490731301@qq.com; 554669561@qq.com;hnahaq@163.com; zhangdayu@zafu.edu.cn).
Tianxiang
T
Zheng
Collaborative Innovation Center of Green Pesticide, Zhejiang Province, Zhejiang A&F University, Linan, Zhejiang, China (190226808@qq.com; 490731301@qq.com; 554669561@qq.com;hnahaq@163.com; zhangdayu@zafu.edu.cn) The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, College of Agricultural and Food Science, Zhejiang A&F University, Linan, Zhejiang, China (554669561@qq.com; hnahaq@163.com; zhangdayu@zafu.edu.cn).
Xiaowen
X
Zheng
Collaborative Innovation Center of Green Pesticide, Zhejiang Province, Zhejiang A&F University, Linan, Zhejiang, China (190226808@qq.com; 490731301@qq.com; 554669561@qq.com;hnahaq@163.com; zhangdayu@zafu.edu.cn) The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, College of Agricultural and Food Science, Zhejiang A&F University, Linan, Zhejiang, China (554669561@qq.com; hnahaq@163.com; zhangdayu@zafu.edu.cn).
Na
N
Han
Institute of Insect Sciences, Zhejiang University, Hangzhou, Zhejiang, China (xxchen@zju.edu.cn).
Xuexin
X
Chen
School of Forestry and Biotechnology, Zhejiang A&F University, Linan, Zhejiang, China (190226808@qq.com; 490731301@qq.com; zhangdayu@zafu.edu.cn) Collaborative Innovation Center of Green Pesticide, Zhejiang Province, Zhejiang A&F University, Linan, Zhejiang, China (190226808@qq.com; 490731301@qq.com; 554669561@qq.com;hnahaq@163.com; zhangdayu@zafu.edu.cn) The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, College of Agricultural and Food Science, Zhejiang A&F University, Linan, Zhejiang, China (554669561@qq.com; hnahaq@163.com; zhangdayu@zafu.edu.cn) zhangdayu@zafu.edu.cn.
Dayu
D
Zhang
D000445:Aldehyde Oxidoreductases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D006430:Hemiptera / Q000145:classification / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008297:Male; D009758:Nymph / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D010802:Phylogeny; D016415:Sequence Alignment
D019476:Insect Proteins; D000445:Aldehyde Oxidoreductases; C021351:hexadecanal dehydrogenase (acylating)
Phenacoccus solenopsis; fatty acyl-CoA reductase; gene expression; spirotetramat