[THE ROLE OF (p)ppGpp MOLECULES IN FORMATION OF "STRICT RESPONSE" IN BACTERIA AND BIOSYNTHESIS OF ANTIBIOTICS AND MORPHOLOGICAL DIFFERENTIATION IN ACTINOMYCETES].
Strict response is a pleiotropic physiological response of cells caused by lack of aminoacetylated tRNAs. Experimentally, this response occurs due to the lack of amino acids in the environment and the limitation of tRNA aminoacylation even in the presence of the corresponding amino acids in the cell. Many features of this response indicate its dependence on the accumulation of ppGpp molecules. There is a correlation between the growth rate of actinomycetes and biosynthesis of their secondary metabolites. Introduction of additional relA gene copies of ppGpp synthetase can affect the production of antibiotics in streptomycetes. The article presents the authors' own experimental data, dedicated to the influence of heterologous relA gene expression in Streptomyces nogalater cells.
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Klymyshin
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Stephanyshyn
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Fedorenko
D039903:Actinobacteria / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D000222:Adaptation, Physiological / Q000235:genetics; D000903:Antibiotics, Antineoplastic / Q000096:biosynthesis*; D015964:Gene Expression Regulation, Bacterial; D007700:Kinetics; D008025:Ligases / Q000235:genetics* / Q000378:metabolism; D009621:Nogalamycin / Q000096:biosynthesis; D012343:RNA, Transfer / Q000235:genetics / Q000378:metabolism*; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics
D000903:Antibiotics, Antineoplastic; D012343:RNA, Transfer; D008025:Ligases; C028812:guanosine 3',5'-polyphosphate synthetases; D009621:Nogalamycin