Pilot-scale UV/H2O2 advanced oxidation process for municipal reuse water: Assessing micropollutant degradation and estrogenic impacts on goldfish (Carassius auratus L.).
Low concentrations (ng/L-μg/L) of emerging micropollutant contaminants in municipal wastewater treatment plant effluents affect the possibility to reuse these waters. Many of those micropollutants elicit endocrine disrupting effects in aquatic organisms resulting in an alteration of the endocrine system. A potential candidate for tertiary municipal wastewater treatment of these micropollutants is ultraviolet (UV)/hydrogen peroxide (H2O2) as an advanced oxidation process (AOP) which was currently applied to treat the secondary effluent of the Gold Bar Wastewater Treatment Plant (GBWWTP) in Edmonton, AB, Canada. A new approach is presented to predict the fluence-based degradation rate constants (kf') of environmentally occurring micropollutants including carbamazepine [(0.87-1.39) × 10(-3) cm(2)/mJ] and 2,4-Dichlorophenoxyacetic acid (2,4-D) [(0.60-0.91) × 10(-3) cm(2)/mJ for 2,4-D] in a medium pressure (MP) UV/H2O2 system based on a previous bench-scale investigation. Rather than using removal rates, this approach can be used to estimate the performance of the MP UV/H2O2 process for degrading trace contaminants of concern found in municipal wastewater. In addition to the ability to track contaminant removal/degradation, evaluation of the MP UV/H2O2 process was also accomplished by identifying critical ecotoxicological endpoints (i.e., estrogenicity) of the treated wastewater. Using quantitative PCR, mRNA levels of estrogen-responsive (ER) genes ERα1, ERα2, ERβ1, ERβ2 and NPR as well as two aromatase encoding genes (CYP19a and CYP19b) in goldfish (Carassius auratus L.) were measured during exposure to the GBWWTP effluent before and after MP UV/H2O2 treatment (a fluence of 1000 mJ/cm(2) and 20 mg/L of H2O2) in spring, summer and fall. Elevated expression of estrogen-responsive genes in goldfish exposed to UV/H2O2 treated effluent (a 7-day exposure) suggested that the UV/H2O2 process may induce acute estrogenic disruption to goldfish principally because of the possible formation of various oxidation by-products. However, prolonged exposure of goldfish (60 days) in UV/H2O2 treated effluent showed a restoration trend of ER gene expressions, especially in the summer. Collectively, our findings provide valuable indications regarding the long-term in vivo assessment of the MP UV/H2O2 process for removing/degrading endocrine disrupting compounds detected in the municipal wastewater effluents.
Department of Civil and Environmental Engineering, University of Alberta, Edmonton, AB, T6G 1H9, Canada.
Zengquan
Z
Shu
Department of Civil and Environmental Engineering, University of Alberta, Edmonton, AB, T6G 1H9, Canada; Department of Biological Sciences, University of Alberta, Edmonton, AB, T6G 2W2, Canada.
Arvinder
A
Singh
Department of Civil and Environmental Engineering, University of Alberta, Edmonton, AB, T6G 1H9, Canada.
Nikolaus
N
Klamerth
Department of Civil and Environmental Engineering, University of Alberta, Edmonton, AB, T6G 1H9, Canada; Department of Civil and Geological Engineering, University of Saskatchewan, Saskatoon, SK, S7N 5A9, Canada.
Kerry
K
McPhedran
Department of Civil and Environmental Engineering, University of Alberta, Edmonton, AB, T6G 1H9, Canada.
James R
JR
Bolton
Department of Civil and Geological Engineering, University of Saskatchewan, Saskatoon, SK, S7N 5A9, Canada.
Miodrag
M
Belosevic
Department of Civil and Environmental Engineering, University of Alberta, Edmonton, AB, T6G 1H9, Canada. Electronic address: mgamalel-din@ualberta.ca.
Mohamed
M
Gamal El-Din
D000818:Animals; D006054:Goldfish / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D010084:Oxidation-Reduction; D014466:Ultraviolet Rays; D014867:Water / Q000378:metabolism; D014874:Water Pollutants, Chemical; D018508:Water Purification
D014874:Water Pollutants, Chemical; D014867:Water; D006861:Hydrogen Peroxide
Aromatase; Endocrine disruption; Estrogen receptors; Fluence-based degradation rate constant; Micropollutants; Pilot-scale UV/H(2)O(2) process