Ionic liquid pretreatment of biomass for sugars production: Driving factors with a plausible mechanism for higher enzymatic digestibility.
In this study, five ionic liquids (ILs) have been explored for biomass pretreatment for the production of fermentable sugar. We also investigated the driving factors responsible for improved enzymatic digestibility of various ILs treated biomass along with postulating the plausible mechanism thereof. Post pretreatment, mainly two factors impacted the enzymatic digestibility (i) structural deformation (cellulose I to II) along with xylan/lignin removal and (ii) properties of ILs; wherein, K-T parameters, viscosity and surface tension had a direct influence on pretreatment. A systematic investigation of these parameters and their impact on enzymatic digestibility is drawn. [C2mim][OAc] with β-value 1.32 resulted 97.7% of glucose yield using 10 FPU/g of biomass. A closer insight into the cellulose structural transformation has prompted a plausible mechanism explaining the better digestibility. The impact of these parameters on the digestibility can pave the way to customize the process to make biomass vulnerable to enzymatic attack.
DBT-IOC Centre for Advanced Bioenergy Research, Research & Development Centre, Indian Oil Corporation Limited, Sector-13, Faridabad 121007, India.
Tirath
T
Raj
DBT-IOC Centre for Advanced Bioenergy Research, Research & Development Centre, Indian Oil Corporation Limited, Sector-13, Faridabad 121007, India.
Ruchi
R
Gaur
DBT-IOC Centre for Advanced Bioenergy Research, Research & Development Centre, Indian Oil Corporation Limited, Sector-13, Faridabad 121007, India.
Pooja
P
Dixit
DBT-IOC Centre for Advanced Bioenergy Research, Research & Development Centre, Indian Oil Corporation Limited, Sector-13, Faridabad 121007, India.
Ravi P
RP
Gupta
Analytical Division, Research & Development Centre, Indian Oil Corporation Limited, Sector-13, Faridabad 121007, India.
V
V
Kagdiyal
DBT-IOC Centre for Advanced Bioenergy Research, Research & Development Centre, Indian Oil Corporation Limited, Sector-13, Faridabad 121007, India. Electronic address: kumarr3@indianoil.in.
Ravindra
R
Kumar
DBT-IOC Centre for Advanced Bioenergy Research, Research & Development Centre, Indian Oil Corporation Limited, Sector-13, Faridabad 121007, India.
Deepak K
DK
Tuli
D018533:Biomass; D002480:Cellulase / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000737:chemistry / Q000378:metabolism; D006868:Hydrolysis; D052578:Ionic Liquids / Q000737:chemistry*; D008031:Lignin / Q000737:chemistry; D000073893:Sugars / Q000737:chemistry* / Q000378:metabolism*; D013500:Surface Tension; D014783:Viscosity; D014990:Xylans / Q000737:chemistry
D052578:Ionic Liquids; D000073893:Sugars; D014990:Xylans; D008031:Lignin; D002480:Cellulase; D005947:Glucose
1-Butyl-3-methylimidazolium acetate (CID: 15953398); 1-Butyl-3-methylimidazolium chloride (CID: 2734161); 1-Ethyl-3-methylimidazolium acetate (CID: 11658353); 4-Nitro analine (CID: 7475); 5-Hydroxymethylfurfural (CID: 237332); Cellulose deformation; Enzymatic digestibility; Glucose (CID: 5793); Ionic liquid; Mechanism; N, N-Diethyl-4-nitroaniline (CID: 75185); Pretreatment; Reichardt's Dye (CID: 2754438); Sulphuric Acid (CID: 1118)