Activated Circulating Myeloid-Derived Suppressor Cells in Patients with Dilated Cardiomyopathy.
OBJECTIVE
Myeloid-derived suppressor cells (MDSCs) are increased in inflammatory and autoimmune disorders. This study aims to evaluate the significance of MDSCs in dilated cardiomyopathy (DCM) patients.


METHODS
In total, 42 newly hospitalized DCM patients and 39 healthy controls were enrolled in the study. The frequencies of circulating CD14+HLA-DR-/low MDSCs were determined by flow cytometry. Then, the functional properties of MDSCs in suppressing T cell proliferation and interferon-gamma (IFN-x03B3;) production were measured in a co-culture model. Then, mRNA expression levels of various important molecules in peripheral blood mononuclear cells were measured by real time polymerase chain reaction. Furthermore, correlation analyses between MDSC frequencies and cardiac function parameters were also performed.


RESULTS
The frequencies of circulating CD14+HLA-DR-/low MDSCs were significantly elevated in DCM patients compared with healthy controls. It showed that MDSCs from DCM patients more effectively suppressed T cell proliferation and IFN-x03B3; production compared with those from healthy controls, which was partially mediated by arginase-1 (Arg-1). In addition, the correlation analysis suggested that MDSC frequencies were negatively correlated with left ventricular ejection fraction (LVEF), while positively with N-terminal pro-brain natriuretic peptide (NT-proBNP) in patients with DCM.


CONCLUSIONS
Circulating activated MDSCs might play significant immunomodulatory roles in the pathogenesis of DCM.
Wen-Cai
WC
Zhang
Yan-Ge
YG
Wang
Wen-Hui
WH
Wei
Xin
X
Xiong
Kan-Ling
KL
Liu
Ding-Hang
DH
Wang
Xiao-Fan
XF
Hu
Yu-Dong
YD
Peng
Jing-Jing
JJ
Wu
Long-Xian
LX
Cheng
D002311:Cardiomyopathy, Dilated / Q000150:complications / Q000276:immunology / Q000473:pathology*; D005260:Female; D006684:HLA-DR Antigens / Q000032:analysis / Q000276:immunology; D006801:Humans; D007108:Immune Tolerance; D007249:Inflammation / Q000150:complications / Q000276:immunology / Q000473:pathology*; D018950:Lipopolysaccharide Receptors / Q000032:analysis / Q000276:immunology; D008297:Male; D008875:Middle Aged; D000072737:Myeloid-Derived Suppressor Cells / Q000276:immunology / Q000473:pathology*; D009206:Myocardium / Q000276:immunology / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology
D006684:HLA-DR Antigens; D018950:Lipopolysaccharide Receptors