Hyaluronic acid grafted PLGA copolymer nanoparticles enhance the targeted delivery of Bromelain in Ehrlich's Ascites Carcinoma.
Rapidly increasing malignant neoplastic disease demands immediate attention. Several dietary compounds have recently emerged as strong anti-cancerous agents. Among, Bromelain (BL), a protease from pineapple plant, was used to enhance its anti-cancerous efficacy using nanotechnology. In lieu of this, hyaluronic acid (HA) grafted PLGA copolymer, having tumor targeting ability, was developed. BL was encapsulated in copolymer to obtain BL-copolymer nanoparticles (NPs) that ranged between 140 to 281nm in size. NPs exhibited higher cellular uptake and cytotoxicity in cells with high CD44 expression as compared with non-targeted NPs. In vivo results on tumor bearing mice showed that NPs were efficient in suppressing the tumor growth. Hence, the formulation could be used as a self-targeting drug delivery cargo for the remission of cancer.
CSIR-Institute of Genomics and Integrative Biology, Delhi University Campus, Mall Road, Delhi 110007, India.
Priyanka
P
Bhatnagar
CSIR-Indian Institute of Toxicology Research, M.G. Marg, Post Box No. 80, Lucknow 226001, U.P., India.
Aditya Bhushan
AB
Pant
CSIR-Indian Institute of Toxicology Research, M.G. Marg, Post Box No. 80, Lucknow 226001, U.P., India.
Yogeshwer
Y
Shukla
National Institute of Immunology (NII), Aruna Asaf Ali Marg, New Delhi 110067, India.
Amulya
A
Panda
CSIR-Institute of Genomics and Integrative Biology, Delhi University Campus, Mall Road, Delhi 110007, India; Department of Biological Sciences and Bioengineering (BSBE) and Centre for Environmental Science and Engineering (CESE), Indian Institute of Technology, Kanpur 208016, India. Electronic address: kcgupta9@gmail.com.
Kailash Chand
KC
Gupta
D000818:Animals; D001963:Bromelains / Q000008:administration & dosage*; D002286:Carcinoma, Ehrlich Tumor / Q000473:pathology*; D045744:Cell Line, Tumor; D004337:Drug Carriers; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D019344:Lactic Acid / Q000737:chemistry*; D008297:Male; D051379:Mice; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D017550:Spectroscopy, Fourier Transform Infrared
D004337:Drug Carriers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D001963:Bromelains; D006820:Hyaluronic Acid
Apoptosis; Bromelain; Ehrlich’s ascites tumors; Hyaluronic acid; Nanoparticles