The Hydroalcoholic Extract of Leaves of Mandevilla moricandiana Induces NO-Mediated Vascular Relaxation.
Natural products extracted from plants represent a valuable source of new bioactive substances. Many studies describe the potential of plant products for the treatment of cardiovascular diseases. Species of theMandevillagenus have been studied for their biological activities, mainly as antioxidant, anti-inflammatory, and vasorelaxant. However, the phytochemical and pharmacological profiles ofMandevilla moricandianahave not been investigated yet. The aim of this study was to evaluate the vasodilator effect of the hydroalcoholic extract of the leaves ofM. moricandiana, as well as its chemical profile. Chemical analysis and quantification of major compounds were performed by HPLC analysis. Total flavonoid content was quantified based on rutin equivalents, and major compounds were identified based on HPLC-DAD-MS analysis.M. moricandianaleaf extract-induced vasodilation was investigated in rat aortic rings precontracted with phenylephrine. The total flavonoids were quantified as 3.25 ± 0.11 % w/w of the hydroalcoholic leaf extract, and HPLC-DAD-MS allowed for the identification of luteolin and quercetin glycosides. The maximal relaxant effect of the hydroalcoholic leaf extract was 86.07 ± 1.68 % at a concentration of 30 µg/mL (p < 0.05; n = 6). The concentration of hydroalcoholic extract of the leaves ofM. moricandiananecessary to reduce phenylephrine-induced contractions of the endothelium-intact aorta by 50 % was 0.82 ± 0.10 µg/mL.M. moricandianaleaf extract-induced vasodilation was abolished in aortas pretreated with NG-nitro-L-arginine methyl ester and 1H-[1,2,4]oxadiazolo-[4,3-α]quinoxalin-1-one. In addition, diphenhydramine partially inhibited the effect of the hydroalcoholic extract of the leaves ofM. moricandiana. Thus,M. moricandiana-induced relaxation depends on the endothelium and on the activation of the nitric oxide/cyclic GMP pathway, with the involvement of endothelial histamine H1receptors. Luteolin and quercetin glycosides seem to contribute to the extract activity.
Laboratório Integrado de Pesquisa, Universidade Federal do Rio de Janeiro - Campus Macaé Professor Aloísio Teixeira, Rio de Janeiro, Brazil.
Letícia Lima Dias Moreira
LL
Ferreira
Laboratório de Produtos Bioativos, IMCT, Universidade Federal do Rio de Janeiro - Campus Macaé Professor Aloísio Teixeira, Rio de Janeiro, Brazil.
Márcio Vinícius
MV
Gomes
Laboratório Integrado de Pesquisa, Universidade Federal do Rio de Janeiro - Campus Macaé Professor Aloísio Teixeira, Rio de Janeiro, Brazil.
Bruno Meirelles
BM
Paes
Laboratório Integrado de Pesquisa, Universidade Federal do Rio de Janeiro - Campus Macaé Professor Aloísio Teixeira, Rio de Janeiro, Brazil.
Paula Lima
PL
do Carmo
Núcleo de Pesquisa em Ecologia e Desenvolvimento Sócio-Ambiental de Macaé, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Tatiana Ungaretti Paleo
TU
Konno
Núcleo de Pesquisa em Ecologia e Desenvolvimento Sócio-Ambiental de Macaé, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Francisco de Assis
FA
Esteves
Departamento de Física e Química da Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil.
Norberto Peporine
NP
Lopes
Departamento de Física e Química da Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil.
José Carlos
JC
Tomaz
Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Ivana Correa Ramos
IC
Leal
Laboratório de Produtos Bioativos, IMCT, Universidade Federal do Rio de Janeiro - Campus Macaé Professor Aloísio Teixeira, Rio de Janeiro, Brazil.
Denise Oliveira
DO
Guimarães
Laboratório de Produtos Bioativos, IMCT, Universidade Federal do Rio de Janeiro - Campus Macaé Professor Aloísio Teixeira, Rio de Janeiro, Brazil.
Michelle Frazão
MF
Muzitano
Laboratório Integrado de Pesquisa, Universidade Federal do Rio de Janeiro - Campus Macaé Professor Aloísio Teixeira, Rio de Janeiro, Brazil.
Juliana Montani
JM
Raimundo
D000818:Animals; D001013:Aorta, Thoracic / Q000187:drug effects; D029062:Apocynaceae / Q000737:chemistry*; D006152:Cyclic GMP / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000098:blood supply / Q000187:drug effects; D019331:NG-Nitroarginine Methyl Ester / Q000009:adverse effects; D009569:Nitric Oxide / Q000378:metabolism; D010656:Phenylephrine / Q000009:adverse effects; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D014662:Vasoconstrictor Agents / Q000009:adverse effects; D014664:Vasodilation / Q000187:drug effects*; D014665:Vasodilator Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
D010936:Plant Extracts; D014662:Vasoconstrictor Agents; D014665:Vasodilator Agents; D010656:Phenylephrine; D009569:Nitric Oxide; D006152:Cyclic GMP; D019331:NG-Nitroarginine Methyl Ester