Aspirin-induced heat stress resistance in chicken myocardial cells can be suppressed by BAPTA-AM in vitro.
Our recent studies have displayed the protective functions of aspirin against heat stress (HS) in chicken myocardial cells, and it may be associated with heat shock proteins (HSPs). In this study, we further investigated the potential role of HSPs in the aspirin-induced heat stress resistance. Four of the most important HSPs including HspB1 (Hsp27), Hsp60, Hsp70, and Hsp90 were induced by aspirin pretreatment and were suppressed by BAPTA-AM. When HSPs were induced by aspirin, much slighter HS injury was detected. But more serious damages were observed when HSPs were suppressed by BAPTA-AM than those cells exposed to HS without BAPTA-AM, even the myocardial cells have been treated with aspirin in prior. Comparing to other HSPs, HspB1 presented the largest increase after aspirin treatments, 86-fold higher than the baseline (the level before HS). These findings suggested that multiple HSPs participated in aspirin's anti-heat stress function but HspB1 may contribute the most. Interestingly, during the experiments, we also found that apoptosis rate as well as the oxidative stress indicators (T-SOD and MDA) was not consistently responding to heat stress injury as expected. By selecting from a series of candidates, myocardial cell damage-related enzymes (CK-MB and LDH), cytopathological tests, and necrosis rate (measured by flow cytometry assays) are believed to be reliable indicators to evaluate heat stress injury in chicken's myocardial cells and they will be used in our further investigations.
College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, Nanjing, 210095, China.
Di
D
Wu
College of Animal Science and Technology, Jinling Institute of Technology, Nanjing, 210038, China.
Miao
M
Zhang
College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, Nanjing, 210095, China.
Yinjun
Y
Lu
College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, Nanjing, 210095, China.
Shu
S
Tang
Institute for Animal Hygiene, Animal Welfare and Farm Animal Behaviour, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany.
N
N
Kemper
Institute for Animal Hygiene, Animal Welfare and Farm Animal Behaviour, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany.
J
J
Hartung
College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, Nanjing, 210095, China. b_endong@njau.edu.cn.
Endong
E
Bao
D000818:Animals; D001241:Aspirin / Q000494:pharmacology*; D030161:Avian Proteins / Q000378:metabolism; D002478:Cells, Cultured; D002645:Chickens; D004353:Drug Evaluation, Preclinical; D004533:Egtazic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D006360:Heat-Shock Proteins / Q000378:metabolism; D018869:Heat-Shock Response / Q000187:drug effects*; D008315:Malondialdehyde / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
D030161:Avian Proteins; D006360:Heat-Shock Proteins; C070379:1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester; D008315:Malondialdehyde; D004533:Egtazic Acid; D013482:Superoxide Dismutase; D001241:Aspirin
Aspirin; BAPTA-AM; HSPs; Heat stress; Inhibitor; Myocardial cell