Effect of plant growth-promoting bacteria on the growth and fructan production of Agave americana L.
The effect of plant growth-promoting bacteria inoculation on plant growth and the sugar content in Agave americana was assessed. The bacterial strains ACO-34A, ACO-40, and ACO-140, isolated from the A. americana rhizosphere, were selected for this study to evaluate their phenotypic and genotypic characteristics. The three bacterial strains were evaluated via plant inoculation assays, and Azospirillum brasilense Cd served as a control strain. Phylogenetic analysis based on the 16S rRNA gene showed that strains ACO-34A, ACO-40 and ACO-140 were Rhizobium daejeonense, Acinetobacter calcoaceticus and Pseudomonas mosselii, respectively. All of the strains were able to synthesize indole-3-acetic acid (IAA), solubilize phosphate, and had nitrogenase activity. Inoculation using the plant growth-promoting bacteria strains had a significant effect (p<0.05) on plant growth and the sugar content of A. americana, showing that these native plant growth-promoting bacteria are a practical, simple, and efficient alternative to promote the growth of agave plants with proper biological characteristics for agroindustrial and biotechnological use and to increase the sugar content in this agave species.
Plant Biotechnology, DEPI Instituto Tecnológico de Tlajomulco, Carretera a San Miguel Cuyutlán, Tlajomulco de Zúñiga, Jalisco, Mexico.
Neyser
N
De La Torre-Ruiz
Laboratory of Biotechnology, Instituto Tecnológico de Tuxtla Gutiérrez, Tuxtla Gutiérrez, Mexico.
Víctor Manuel
VM
Ruiz-Valdiviezo
Laboratory of Biotechnology, Instituto Tecnológico de Tuxtla Gutiérrez, Tuxtla Gutiérrez, Mexico.
Clara Ivette
CI
Rincón-Molina
Plant Biotechnology, DEPI Instituto Tecnológico de Tlajomulco, Carretera a San Miguel Cuyutlán, Tlajomulco de Zúñiga, Jalisco, Mexico.
Martha
M
Rodríguez-Mendiola
Plant Biotechnology, DEPI Instituto Tecnológico de Tlajomulco, Carretera a San Miguel Cuyutlán, Tlajomulco de Zúñiga, Jalisco, Mexico.
Carlos
C
Arias-Castro
Laboratory of Biotechnology, Instituto Tecnológico de Tuxtla Gutiérrez, Tuxtla Gutiérrez, Mexico.
Federico Antonio
FA
Gutiérrez-Miceli
Laboratory of Biotechnology, Instituto Tecnológico de Tuxtla Gutiérrez, Tuxtla Gutiérrez, Mexico.
Héctor
H
Palomeque-Dominguez
Laboratory of Biotechnology, Instituto Tecnológico de Tuxtla Gutiérrez, Tuxtla Gutiérrez, Mexico. Electronic address: reriro61@hotmail.com.
Reiner
R
Rincón-Rosales
D027882:Agave / Q000382:microbiology* / Q000502:physiology*; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000378:metabolism*; D005630:Fructans / Q000096:biosynthesis*; D005838:Genotype; D010641:Phenotype; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000096:biosynthesis*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D005630:Fructans; D010937:Plant Growth Regulators; D012336:RNA, Ribosomal, 16S
16S rRNA; Agave; Bacteria; Inoculation; Inulin