Domain I of the 5' non-translated genomic region in coxsackievirus B3 RNA is not required for productive replication.
Domain I is a cloverleaf-like secondary structure at the 5' termini of all enterovirus genomes, comprising part of a cis-acting replication element essential for efficient enteroviral replication. 5' genomic terminal deletions up to as much as 55% of domain I can occur without lethality following coxsackie B virus infections. We report here that the entire CVB structural domain I can be deleted without lethality.
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198-6495, USA; Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, NE 68198-5830, USA.
L
L
Jaramillo
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198-6495, USA; Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
S
S
Smithee
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198-6495, USA.
S
S
Tracy
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198-6495, USA. Electronic address: nchapman@unmc.edu.
N M
NM
Chapman
D020121:5' Untranslated Regions; D001483:Base Sequence; D002460:Cell Line; D002478:Cells, Cultured; D029822:Enterovirus B, Human / Q000502:physiology*; D016679:Genome, Viral; D006801:Humans; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D017384:Sequence Deletion; D014779:Virus Replication
D020121:5' Untranslated Regions; D012367:RNA, Viral
5′ terminal deletion; Cloverleaf; Coxsackievirus; Domain I; Enterovirus; RNA structure