Outbreak Investigation of NADC30-Like PRRSV in South-East China.
Epidemiological outbreak investigations were conducted on NADC30-like porcine reproductive and respiratory syndrome virus (PRRSV) to investigate the prevalence of the disease in south-east China in 2015. Two more provinces were found to have NADC30-like PRRSV circulating besides previously reported six provinces. Phylogenetic analysis showed that these virus isolates were clustered in an independent branch and shared high nucleotide similarity to NADC30, a type 2 PRRSV that has been isolated in Unite States in 2008. One NADC30-like PRRSV strain from Henan province was successfully isolated on porcine alveolar macrophages and was tested on 6-week-old specific pathogen-free pigs for pathogenic study. The virus-inoculated pigs showed typical PRRSV clinical symptoms, but all pigs survived throughout the study with a period of 14 days. At necropsy, the lungs of infected pigs developed PRRSV-specific interstitial pneumonia, and virus antigen was detected in lung samples. Therefore, our results indicated NADC30-like PRRSV has widely spread in China and could cause clinical disease on pigs.
National Research Center for Veterinary Medicine, Luoyang, China.
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Li
National Research Center for Veterinary Medicine, Luoyang, China.
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Zhuang
National Research Center for Veterinary Medicine, Luoyang, China.
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Wang
National Research Center for Veterinary Medicine, Luoyang, China.
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Han
National Research Center for Veterinary Medicine, Luoyang, China.
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Sun
National Research Center for Veterinary Medicine, Luoyang, China.
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Xiao
National Research Center for Veterinary Medicine, Luoyang, China.
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Ji
National Research Center for Veterinary Medicine, Luoyang, China.
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Li
National Research Center for Veterinary Medicine, Luoyang, China.
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Tan
National Research Center for Veterinary Medicine, Luoyang, China.
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Li
National Research Center for Veterinary Medicine, Luoyang, China.|College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China.|OIE Porcine Reproductive and Respiratory Syndrome Reference Laboratory, Beijing, China.
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Tian
D000818:Animals; D000956:Antigens, Viral / Q000378:metabolism; D002681:China; D004196:Disease Outbreaks; D008168:Lung / Q000378:metabolism / Q000473:pathology; D017563:Lung Diseases, Interstitial / Q000473:pathology / Q000821:virology; D010802:Phylogeny; D019318:Porcine Reproductive and Respiratory Syndrome / Q000453:epidemiology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics / Q000276:immunology; D013552:Swine
D000956:Antigens, Viral
HNjz15; NADC30; epidemiology; pathogenesis; porcine reproductive and respiratory syndrome virus