Anti-inflammatory effect of taurocholate on TNBS-induced ulcerative colitis in mice.
Taurocholate is a natural conjugated bile acid. The aim of this study was to evaluate the anti-inflammatory effect of taurocholate in TNBS-induced ulcerative colitis in mice. The colitis were induced by rectal administration of TNBS. After 24h, the experimental animals were treated with sulfasalazine (SASP, 500mg/kg/day) and taurocholate (20, 40 and 60mg/kg) for 7 consecutive days. The anti-inflammatory effects of taurocholate for colitis were assessed by body weight, colonic weight and length, macroscopic scores, and histopathological examinations. In addition, the colonic tissue levels of myeloperoxidase (MPO) activity, interleukin (IL)-1β, interferon (IFN-γ) and tumour necrosis factor-α (TNF-α) were also determined to assess the effect of taurocholate. Compared with the model group, treatment with taurocholate (20, 40 and 60mg/kg) significantly inhibited the body weight loss, improved colonic weight and length, and decreased macroscopic and histopathological scores. Furthermore, the activity accumulation of MPO and the colonic tissue levels of IL-1β, IFN-γ and TNF-α were also decreased by administration of taurocholate. All the findings of this study suggested that taurocholate has the anti-inflammatory effect in ulcerative colitis in mice and indicated it as a good candidate to treat inflammatory bowel disease.
Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi⿿an 710069, China; Biomedicine Key Laboratory of Shaanxi Province, College of Life Science, Northwest University, Xi⿿an 710069, China.
Yang
Y
Yang
Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi⿿an 710069, China; Biomedicine Key Laboratory of Shaanxi Province, College of Life Science, Northwest University, Xi⿿an 710069, China. Electronic address: hejiao@nwu.edu.cn.
Jiao
J
He
Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi⿿an 710069, China.
Yuan
Y
Suo
Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi⿿an 710069, China.
Le
L
Lv
Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi⿿an 710069, China.
Jingjing
J
Wang
Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi⿿an 710069, China.
Chuanchuan
C
Huo
Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi⿿an 710069, China.
Zongwei
Z
Zheng
Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi⿿an 710069, China.
Ziye
Z
Wang
Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi⿿an 710069, China.
Jing
J
Li
Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi⿿an 710069, China.
Wenji
W
Sun
Institut Parisien de Chimie Moléculaire, CNRS UMR 8232, Université Pierre et Marie Curie-Paris 6, 4 place Jussieu, 75005 Paris, France.
Yongmin
Y
Zhang
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D001835:Body Weight / Q000187:drug effects; D003093:Colitis, Ulcerative / Q000139:chemically induced* / Q000188:drug therapy* / Q000473:pathology; D003106:Colon / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D007371:Interferon-gamma / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D008297:Male; D008807:Mice, Inbred BALB C; D009195:Peroxidase / Q000378:metabolism; D012460:Sulfasalazine / Q000494:pharmacology / Q000627:therapeutic use; D015996:Survival Rate; D013656:Taurocholic Acid / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D014302:Trinitrobenzenesulfonic Acid; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D012460:Sulfasalazine; D013656:Taurocholic Acid; D007371:Interferon-gamma; D014302:Trinitrobenzenesulfonic Acid; D009195:Peroxidase
Anti-inflammatory effect; Cytokines; TNBS; Taurocholate; Ulcerative colitis