Association of TNFα-308, IFNγ+874, and IL10-1082gene polymorphisms and the risk of non-small cell lung cancer in the population of the South Indian state of Telangana.
BACKGROUND
Cytokine-mediated inflammation is important in the pathogenesis of non-small cell lung cancer (NSCLC). Genetic polymorphisms in cytokine genes and their association with lung cancer in the Indian population have not been reported.


METHODS
For the first time, we analyzed genetic polymorphisms of TNFα-308, IFNγ+874, and IL10-1082genes in 246 NSCLC patients and 250 healthy controls in the South Indian population from Telangana using ARMS PCR.


RESULTS
IFNγ+874A/T and IL10-1082G/G gene polymorphisms were found to be significantly associated with NSCLC with 1.56- and 1.68-fold disease risk, respectively. There was no association between the risk of NSCLC and TNFα-308polymorphism. Gene polymorphisms stratified according to smoking revealed that IFNγ+874A/T polymorphisms in smokers increased the disease risk by 2.91 fold. IL10-1082G/G polymorphisms showed 2-fold increased risk among patients who were smokers when compared to the controls. However, there was no association between TNFα-308, IFNγ+874, and IL10-1082gene polymorphism and the stage of the NSCLC patients. The overall risk associated with the combination of these polymorphisms indicated that the TNFα-308G/A + IFNγ+874A/T + IL10-1082G/G genotype increased the risk by 1.5 fold.


CONCLUSIONS
The results of our study indicate an association between cytokine gene polymorphisms and the risk of NSCLC in an Indian population.
Institute of Genetics and Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad, 500016, Telangana, India. vidyullatha.p@gmail.com.|Department of Biotechnology and Bioinformatics, University of Hyderabad, Gachibowli, Hyderabad, 500046, Telangana, India. vidyullatha.p@gmail.com.
Vidyullatha
V
Peddireddy
Institute of Genetics and Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad, 500016, Telangana, India.
Siva Prasad
SP
Badabagni
Bhagwan Mahavir Medical and Research Centre, Hyderabad, 500004, Telangana, India.
Shehnaz
S
Sulthana
Institute of Genetics and Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad, 500016, Telangana, India.
Venkata Karunakar
VK
Kolla
Indo-American Cancer Hospital, Banjara Hills, Hyderabad, 500034, Telangana, India.
Sandhya Devi
SD
Gundimeda
Institute of Genetics and Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad, 500016, Telangana, India.
Hemaprasad
H
Mundluru
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology*; D016022:Case-Control Studies; D005260:Female; D005838:Genotype; D006801:Humans; D007194:India; D007371:Interferon-gamma / Q000235:genetics*; D016753:Interleukin-10 / Q000235:genetics*; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D012907:Smoking; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007371:Interferon-gamma
Gene polymorphism; Interferon-γ; Interleukin-10; Non-small cell lung cancer; Tumor necrosis factor-α