Elevated levels of FN1 and CCL2 in bronchoalveolar lavage fluid from sarcoidosis patients.
Sarcoidosis is a granulomatous systemic inflammatory disease in which more than 90 % of all patients develop pulmonary manifestations. Several gene associations have previously been described, but established and clinically useful biomarkers are still absent. This study aimed to find proteins in bronchoalveolar lavage (BAL) fluid that can be associated with the disease.



We developed and performed profiling of 94 selected proteins in BAL fluid and serum samples obtained from newly diagnosed and non-treated patients with sarcoidosis. Using multiplexed immunoassays, a total of 317 BAL and 217 serum samples were analyzed, including asthmatic patients and healthy individuals as controls.



Our analyses revealed increased levels of eight proteins in sarcoidosis patients compared to controls. Out of these, fibronectin (FN1) and C-C motif chemokine 2 (CCL2) revealed the strongest associations. In addition, cadherin 5 (CDH5) was found to correlate positively with lymphocyte cell numbers in BAL fluid.



Applying a high throughput proteomics screening technique, we found proteins of potential clinical relevance in the context of sarcoidosis.
Immunology and Allergy Unit, Department of Medicine Solna, Karolinska Institutet and University Hospital, Stockholm, Sweden. carl.hamsten@ki.se.
Carl
C
Hamsten
Respiratory Medicine Unit, Department of Medicine Solna and CMM, Karolinska Institutet and Karolinska University Hospital, Stockholm, Sweden.
Emil
E
Wiklundh
Therapeutic Immune Design Unit, Department of Clinical neuroscience, Center for Molecular Medicine, Karolinska Institutet, Stockholm, Sweden.
Hans
H
Grönlund
Affinity proteomics, SciLifeLab, School of Biotechnology, KTH - Royal Institute of Technology, Stockholm, Sweden.
Jochen M
JM
Schwenk
Affinity proteomics, SciLifeLab, School of Biotechnology, KTH - Royal Institute of Technology, Stockholm, Sweden.
Mathias
M
Uhlén
Respiratory Medicine Unit, Department of Medicine Solna and CMM, Karolinska Institutet and Karolinska University Hospital, Stockholm, Sweden.
Anders
A
Eklund
Affinity proteomics, SciLifeLab, School of Biotechnology, KTH - Royal Institute of Technology, Stockholm, Sweden.
Peter
P
Nilsson
Respiratory Medicine Unit, Department of Medicine Solna and CMM, Karolinska Institutet and Karolinska University Hospital, Stockholm, Sweden. johan.grunewald@ki.se.
Johan
J
Grunewald
Affinity proteomics, SciLifeLab, School of Biotechnology, KTH - Royal Institute of Technology, Stockholm, Sweden. anna.haggmark@scilifelab.se.
Anna
A
Häggmark-Månberg
D000293:Adolescent; D000328:Adult; D000368:Aged; D019540:Area Under Curve; D015415:Biomarkers / Q000032:analysis; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D016022:Case-Control Studies; D018932:Chemokine CCL2 / Q000032:analysis* / Q000097:blood; D005260:Female; D005353:Fibronectins / Q000032:analysis* / Q000097:blood; D057166:High-Throughput Screening Assays; D006801:Humans; D007118:Immunoassay; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D040901:Proteomics / Q000379:methods; D012372:ROC Curve; D017565:Sarcoidosis, Pulmonary / Q000097:blood / Q000175:diagnosis / Q000378:metabolism*; D015854:Up-Regulation; D055815:Young Adult
D015415:Biomarkers; C111835:CCL2 protein, human; D018932:Chemokine CCL2; C067625:FN1 protein, human; D005353:Fibronectins
Bronchoalveolar lavage; Löfgren’s syndrome; Protein profiling; Sarcoidosis