Injection of Syngeneic Murine Melanoma Cells to Determine Their Metastatic Potential in the Lungs.
Approximately 90% of human cancer deaths are linked to metastasis. Despite the prevalence and relative harm of metastasis, therapeutics for treatment or prevention are lacking. We report a method for the establishment of pulmonary metastases in mice, useful for the study of this phenomenon. Tail vein injection of B57BL/6J mice with B16-BL6 is among the most used models for melanoma metastases. Some of the circulating tumor cells establish themselves in the lungs of the mouse, creating "experimental" metastatic foci. With this model it is possible to measure the relative effects of therapeutic agents on the development of cancer metastasis. The difference in enumerated lung foci between treated and untreated mice indicates the efficacy of metastases neutralization. However, prior to the investigation of a therapeutic agent, it is necessary to determine an optimal number of injected B16-BL6 cells for the quantitative analysis of metastatic foci. Injection of too many cells may result in an overabundance of metastatic foci, impairing proper quantification and overwhelming the effects of anti-cancer therapies, while injection of too few cells will hinder the comparison between treated and controls.
Brain Tumor Center & Neuro-Oncology Unit, Department of Neurology, Beth Israel Deaconess Medical Center & Harvard Medical School.
Joshua J
JJ
Timmons
Division of Cancer Genetics, Department of Medicine, Beth Israel Deaconess Medical Center & Harvard Medical School.
Sean
S
Cohessy
Brain Tumor Center & Neuro-Oncology Unit, Department of Neurology, Beth Israel Deaconess Medical Center & Harvard Medical School; ewong@bidmc.harvard.edu.
Eric T
ET
Wong
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D008175:Lung Neoplasms / Q000097:blood / Q000473:pathology / Q000556:secondary*; D008546:Melanoma, Experimental / Q000097:blood / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation / Q000379:methods; D009360:Neoplastic Cells, Circulating / Q000473:pathology