Transcriptome profiling of the salt-stress response in Triticum aestivum cv. Kharchia Local.
Kharchia Local wheat variety is an Indian salt tolerant land race known for its tolerance to salinity. However, there is a lack of detailed information regarding molecular mechanism imparting tolerance to high salinity in this bread wheat. In the present study, differential root transcriptome analysis identifying salt stress responsive gene networks and functional annotation under salt stress in Kharchia Local was performed. A total of 453,882 reads were obtained after quality filtering, using Roche 454-GS FLX Titanium sequencing technology. From these reads 22,241 ESTs were generated out of which, 17,911 unigenes were obtained. A total of 14,898 unigenes were annotated against nr protein database. Seventy seven transcription factors families in 826 unigenes and 11,002 SSRs in 6,939 unigenes were identified. Kyoto Encyclopedia of Genes and Genomes database identified 310 metabolic pathways. The expression pattern of few selected genes was compared during the time course of salt stress treatment between salt-tolerant (Kharchia Local) and susceptible (HD2687). The transcriptome data is the first report, which offers an insight into the mechanisms and genes involved in salt tolerance. This information can be used to improve salt tolerance in elite wheat cultivars and to develop tolerant germplasm for other cereal crops.
Banasthali University, Banasthali, Rajasthan, India.|Biotechnology and Climate Change Laboratory, ICAR-NRC on Plant Biotechnology, New Delhi, India.
Etika
E
Goyal
Biotechnology and Climate Change Laboratory, ICAR-NRC on Plant Biotechnology, New Delhi, India.
Singh K
SK
Amit
Biotechnology and Climate Change Laboratory, ICAR-NRC on Plant Biotechnology, New Delhi, India.
Ravi S
RS
Singh
Biotechnology and Climate Change Laboratory, ICAR-NRC on Plant Biotechnology, New Delhi, India.
Ajay K
AK
Mahato
Banasthali University, Banasthali, Rajasthan, India.|Devi Ahilya University, Indore, India.
Suresh
S
Chand
Biotechnology and Climate Change Laboratory, ICAR-NRC on Plant Biotechnology, New Delhi, India.
Kumar
K
Kanika
D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant; D053263:Gene Regulatory Networks; D058977:Molecular Sequence Annotation; D018517:Plant Roots / Q000235:genetics; D055049:Salt Tolerance; D017423:Sequence Analysis, RNA; D014908:Triticum / Q000235:genetics*