Rapid detection of lineage IV peste des petits ruminants virus by real-time RT-PCR.
Peste des petits ruminants virus (PPRV) is the cause agent of peste des petitis ruminants (PPR). A novel lineage IV PPRV has reemerged in China in 2013 and 2014. Mass vaccination was implemented in most provinces in China. In order to detect lineage IV PPRV in clinical samples and to distinguish rapidly it from the other lineages PPRVs, a real-time RT-PCR assay was developed. This assay showed high sensitivity, specificity and efficiency in differentiating the lineage IV PPRV from others. The performance of this assay was evaluated by positive clinical samples of lineage IV viruses. This new real-time RT-PCR assay will facilitate epidemiological investigations and rapid differentiatial diagnosis in areas where lineage IV viruses are circulating.
National Research Center for Exotic Animal Diseases, China Animal Health and Epidemiology Center, Qingdao, Shandong 266032, PR China.
Lin
L
Li
National Research Center for Exotic Animal Diseases, China Animal Health and Epidemiology Center, Qingdao, Shandong 266032, PR China.
Xiaodong
X
Wu
National Research Center for Exotic Animal Diseases, China Animal Health and Epidemiology Center, Qingdao, Shandong 266032, PR China.
Fuxiao
F
Liu
National Research Center for Exotic Animal Diseases, China Animal Health and Epidemiology Center, Qingdao, Shandong 266032, PR China. Electronic address: wangzhiliang@cahec.cn.
Zhiliang
Z
Wang
National Research Center for Exotic Animal Diseases, China Animal Health and Epidemiology Center, Qingdao, Shandong 266032, PR China.
Chunju
C
Liu
National Research Center for Exotic Animal Diseases, China Animal Health and Epidemiology Center, Qingdao, Shandong 266032, PR China.
Qinghua
Q
Wang
National Research Center for Exotic Animal Diseases, China Animal Health and Epidemiology Center, Qingdao, Shandong 266032, PR China.
Jingyue
J
Bao
D000818:Animals; D017931:DNA Primers; D003937:Diagnosis, Differential; D004196:Disease Outbreaks / Q000662:veterinary; D029021:Peste-des-Petits-Ruminants / Q000175:diagnosis* / Q000821:virology; D018107:Peste-des-petits-ruminants virus / Q000235:genetics* / Q000302:isolation & purification; D012367:RNA, Viral; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D017931:DNA Primers; D012367:RNA, Viral
Differentiatial diagnosis; Lineage IV; Peste des petits ruminants virus; Real-time RT-PCR