DNA Extraction: Organic and Solid-Phase.
DNA extraction remains a critical step in DNA profiling of biological material recovered from scenes of crime. In the forensic community several methods have gained popularity, including Chelex(®), organic extraction, and solid-phase extraction. While some laboratories streamlined their processes and only use one method we have retained several methods and continue to use these for different sample types. In this chapter we present three methods that have been used for several years in our laboratory.
Forensic Evidence Department, Abu Dhabi Police, P.O. Box 253, Abu Dhabi, United Arab Emirates. tayyari_2001@hotmail.com.
Wafa
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Altayari
D002725:Chloroform / Q000737:chemistry*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D053803:Forensic Genetics; D010636:Phenols / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D052616:Solid Phase Extraction / Q000379:methods*
D010636:Phenols; D002725:Chloroform; D004247:DNA
DNA extraction; EZ1® DNA Investigator®; Phenol–chloroform; PrepFiler®; Solid-phase extraction