Kit receptor tyrosine kinase dysregulations in feline splenic mast cell tumours.
This study investigated Kit receptor dysregulations (cytoplasmic immunohistochemical expression and/or c-KIT mutations) in cats affected with splenic mast cell tumours. Twenty-two cats were included. Median survival time was 780 days (range: 1-1219). An exclusive splenic involvement was significantly (P = 0.042) associated with longer survival (807 versus 120 days). Eighteen tumours (85.7%) showed Kit cytoplasmic expression (Kit pattern 2, 3). Mutation analysis was successful in 20 cases. Fourteen missense mutations were detected in 13 out of 20 tumours (65%). Eleven (78.6%) were located in exon 8, and three (21.6%) in exon 9. No mutations were detected in exons 11 and 17. Seven mutations corresponded to the same internal tandem duplication in exon 8 (c.1245_1256dup). Although the association between Kit cytoplasmic expression and mutations was significant, immunohistochemistry cannot be considered a surrogate marker for mutation analysis. No correlation was observed between c-Kit mutations and tumour differentiation, mitotic activity or survival.
Department of Veterinary Medical Sciences, Alma Mater Studiorum - University of Bologna, Bologna, Italy.
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S
Sabattini
Department of Veterinary Medical Sciences, Alma Mater Studiorum - University of Bologna, Bologna, Italy.
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G
Barzon
Department of Comparative Biomedicine and Food Science, University of Padua, Padua, Italy.
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M
Giantin
Department of Comparative Biomedicine and Food Science, University of Padua, Padua, Italy.
R M
RM
Lopparelli
Department of Comparative Biomedicine and Food Science, University of Padua, Padua, Italy.
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M
Dacasto
Laboratoire IDEXX, Saint Denis, France.
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D
Prata
Department of Veterinary Medical Sciences, Alma Mater Studiorum - University of Bologna, Bologna, Italy.
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G
Bettini
D000818:Animals; D002371:Cat Diseases / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D002415:Cats; D005260:Female; D008297:Male; D008415:Mastocytosis / Q000201:enzymology / Q000235:genetics / Q000378:metabolism / Q000662:veterinary*; D009154:Mutation / Q000235:genetics; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics / Q000378:metabolism*; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D013160:Splenic Neoplasms / Q000201:enzymology / Q000235:genetics / Q000378:metabolism / Q000662:veterinary*
D019009:Proto-Oncogene Proteins c-kit; D020794:Receptor Protein-Tyrosine Kinases
CD117; Kit; feline; mast cell tumour; spleen; tyrosine kinase