Doxorubicin-loaded fucoidan capped gold nanoparticles for drug delivery and photoacoustic imaging.
Polymer nanoparticles are emerging as a useful tool for a wide variety of biomedical and therapeutic applications. The present study demonstrates the multifunctional doxorubicin-loaded fucoidan capped gold nanoparticles (DOX-Fu AuNPs) for drug delivery and photoacoustic imaging (PAI). Biocompatible AuNPs were synthesized using a naturally occurring fucoidan (Fu) as a capping and reducing agent. The Fu AuNPs synthesis was determined using UV-visible spectrum, and it was further characterized using high resolution transmission electron microscopy, energy dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction analysis. The release of DOX from DOX-Fu AuNPs was greater in acidic pH (4.5) than in neutral pH (7.4). The in vitro cytotoxic effect of fucoidan, Fu AuNPs, DOX, and DOX-Fu AuNPs inhibited the proliferation of human breast cancer cells with an inhibitory concentration of 35μg/mL, 30μg/mL, 15μg/mL, and 5μg/mL at 24h. DOX-Fu AuNPs induced both early and late apoptosis in a concentration-dependent manner compared with untreated control cells. The ability of DOX-Fu AuNPs as a contrast agent for in vitro breast cancer imaging with PAI has been evaluated. These results suggest that the multifunctional DOX-Fu AuNPs for drug delivery and PAI can soon provide considerable contribution to human health.
Marine-Integrated Bionics Research Center, Pukyong National University, Busan 608-737, Republic of Korea.
Panchanathan
P
Manivasagan
Marine-Integrated Bionics Research Center, Pukyong National University, Busan 608-737, Republic of Korea.
Subramaniyan
S
Bharathiraja
Department of Biomedical Engineering and Center for Marine-Integrated Biotechnology (BK21 Plus), Pukyong National University, Busan 608-737, Republic of Korea.
Nhat Quang
NQ
Bui
Marine-Integrated Bionics Research Center, Pukyong National University, Busan 608-737, Republic of Korea.
Bian
B
Jang
Marine-Integrated Bionics Research Center, Pukyong National University, Busan 608-737, Republic of Korea.
Yun-Ok
YO
Oh
Department of Mechanical Engineering, Myong-Ji University, San 38-2, Nam-Dong, Cheoin-Gu, Yongin-Si, Gyeonggi-Do 449-728, Republic of Korea.
In Gweon
IG
Lim
Marine-Integrated Bionics Research Center, Pukyong National University, Busan 608-737, Republic of Korea; Department of Biomedical Engineering and Center for Marine-Integrated Biotechnology (BK21 Plus), Pukyong National University, Busan 608-737, Republic of Korea. Electronic address: jungoh@pknu.ac.kr.
Junghwan
J
Oh
D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000494:pharmacology*; D016503:Drug Delivery Systems / Q000379:methods*; D065546:Drug Liberation; D005434:Flow Cytometry; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D019047:Phantoms, Imaging; D061088:Photoacoustic Techniques / Q000379:methods*; D011134:Polysaccharides / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared
D011134:Polysaccharides; D006046:Gold; D004317:Doxorubicin; C007789:fucoidan
Doxorubicin; Drug delivery; Fucoidan; Gold nanoparticles; Photoacoustic imaging