Effects of Deep Cooling and Re-Warming on Ionotropic Glutamatergic Receptors In Vitro.
We studied the effects of cooling to -10°C and re-warming to 37°C on slices of rat olfactory cortex. The amplitudes of action potential in the lateral olfactory tract and excitatory postsynaptic potential activated by AMPA recovered during slow cooling/re-warming (0.1°C/min), while during rapid cooling/re-warming (9°C/min), they surpassed the control values. NMDA receptor-dependent mechanism was blocked in both cooling/re-warming modes. Swelling of the brain slices was observed during re-warming, especially during rapid cooling/re-warming. Nerve fibers of the lateral olfactory tract and AMPA-related processes survived deep cooling/re-warming, while NMDA-related processes were irreversibly blocked.
Laboratory of Regulation for Functions of Brain Neurons, I. P. Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg, Russia. mok@inbox.ru.
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Mokrushin
D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000502:physiology; D003080:Cold Temperature; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology; D066298:In Vitro Techniques; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D058468:Receptors, Ionotropic Glutamate / Q000378:metabolism*; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism; D017694:Rewarming; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / Q000378:metabolism
D058468:Receptors, Ionotropic Glutamate; D016194:Receptors, N-Methyl-D-Aspartate; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
brain slices; cooling; re-warming