Supplemented base medium containing Amburana cearensis associated with FSH improves in vitro development of isolated goat preantral follicles.
The effects of Amburana cearensis ethanolic extract, with or without addition of a mix of supplements associated or not with FSH, on in vitro morphology and development of caprine secondary follicles were evaluated. In experiment 1, isolated follicles (250 μm in diameter) were cultured for 12 days in alpha-modified minimal essential medium (α-MEM) alone (control) or in medium composed of different concentrations of A. cearensis extract (Amb 0.1; 0.2, or 0.4 mg/mL). In experiment 2, culture media were α-MEM or Amb 0.2 mg/mL (both without supplements), or these same media supplemented with BSA, insulin, transferrin, selenium, glutamine, hypoxanthine, and ascorbic acid (referred as α-MEM(+) and Amb 0.2(+), respectively), or these last groups also supplemented with sequential FSH (100 ng/mL from Day 0 to Day 6; 500 ng/mL from Day 6 to Day 12), constituting groups α-MEM(+) + FSH and Amb 0.2(+) + FSH. At the end of culture in experiment 1, control medium (α-MEM) and Amb 0.2 mg/mL had higher percentages (P < 0.05) of morphologically normal follicles and percentage of fully grown oocytes, i.e., oocyte greater than 110 μm, compared to the other A. cearensis extract concentrations. In experiment 2, all supplemented media had higher percentages (P < 0.05) of normal follicles and antrum formation than nonsupplemented media. In addition, follicles cultured in Amb 0.2(+) + FSH showed an average increase in diameter higher (P < 0.05) than the other treatments. Oocytes cultured in both treatments supplemented with FSH showed greater glutathione and active mitochondria levels than nonsupplemented media but similar to the other treatments. In conclusion, A. cearensis extract (0.2 mg/mL) added by supplements and FSH improves follicular growth. Therefore, it can be an alternative culture medium for goat preantral follicle development.
Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of San Francisco Valley, Petrolina, PE, Brazil.
B B
BB
Gouveia
Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of San Francisco Valley, Petrolina, PE, Brazil.
T J S
TJ
Macedo
Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of San Francisco Valley, Petrolina, PE, Brazil.
J M S
JM
Santos
Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of San Francisco Valley, Petrolina, PE, Brazil.
R S
RS
Barberino
Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of San Francisco Valley, Petrolina, PE, Brazil.
V G
VG
Menezes
Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of San Francisco Valley, Petrolina, PE, Brazil.
M C
MC
Müller
Center for Studies and Research on Medicinal Plants, Federal University of San Francisco Valley, Petrolina, PE, Brazil.
J R G S
JR
Almeida
Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), Faculty of Veterinary, State University of Ceará, Fortaleza, CE, Brazil.
J R
JR
Figueiredo
Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of San Francisco Valley, Petrolina, PE, Brazil. Electronic address: helena.matos@univasf.edu.br.
M H T
MH
Matos
D000818:Animals; D007887:Fabaceae / Q000737:chemistry*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D005978:Glutathione; D006041:Goats; D020287:In Situ Nick-End Labeling; D059471:In Vitro Oocyte Maturation Techniques; D008928:Mitochondria; D009865:Oocytes / Q000187:drug effects* / Q000502:physiology; D006080:Ovarian Follicle / Q000187:drug effects* / Q000502:physiology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D046509:Tissue Culture Techniques
D010936:Plant Extracts; D005640:Follicle Stimulating Hormone; D005978:Glutathione
Antioxidant; Glutathione; Medicinal plant; Mitochondria; Oocyte