Extraction, purification and anti-radiation activity of persimmon tannin from Diospyros kaki L.f.
In this study, persimmon tannin was extracted from Diospyros kaki L.f. using ultrasound-assisted extraction and purified by D101 macroporous resin column chromatography and polysulfone ultrafiltration membrane. The tannin content of the final persimmon tannin extracts was attained to 39.56% calculated as catechin equivalents. Also, the radioprotective effects of persimmon tannin for HEK 293T cells proliferation and apoptosis after Gamma irradiation were investigated by CCK-8, Hoechst 33258 staining, flow cytometry assay and intracellular reactive oxygen species assay (ROS). Persimmon tannin was pre-incubated with HEK 293T cells for 12 h prior to Gamma irradiation. It was found that pretreatment with persimmon tannin increased cell viability and inhibited generation of Gamma-radiation induced ROS in HEK 293T cells exposed to 8 Gy Gamma-radiation. The percentage of apoptotic cells were only 6.7% when the radiation dose was 8 Gy and pretreated with 200 μg/ml of persimmon tannin. All these results indicated that persimmon tannin offered a potent radioprotective effect on cell vitality and cell apoptosis of Gamma-radiation exposure in HEK 293T cells. This study would serve as a pre-clinical evaluation of persimmon tannin for use in people with radiation protection.
School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, Guangxi 541014, China.
Zhide
Z
Zhou
School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, Guangxi 541014, China.
Yong
Y
Huang
School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, Guangxi 541014, China.
Jintao
J
Liang
Guangxi Key Laboratory of Metabolic Disease Research, Guilin 181st Hospital, Guilin, Guangxi 541014, China.
Minglin
M
Ou
Guangxi Key Laboratory of Metabolic Disease Research, Guilin 181st Hospital, Guilin, Guangxi 541014, China.
Jiejing
J
Chen
School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, Guangxi 541014, China. Electronic address: liguiyin01@163.com.
Guiyin
G
Li
D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D029788:Diospyros / Q000737:chemistry*; D005720:Gamma Rays; D006801:Humans; D011837:Radiation-Protective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D013634:Tannins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
D011837:Radiation-Protective Agents; D017382:Reactive Oxygen Species; D013634:Tannins
Anti-radiation; Persimmon tannin; Purification; Ultrasound-assisted extraction