Cell Deformation by Single-beam Acoustic Trapping: A Promising Tool for Measurements of Cell Mechanics.
We demonstrate a noncontact single-beam acoustic trapping method for the quantification of the mechanical properties of a single suspended cell with label-free. Experimentally results show that the single-beam acoustic trapping force results in morphological deformation of a trapped cell. While a cancer cell was trapped in an acoustic beam focus, the morphological changes of the immobilized cell were monitored using bright-field imaging. The cell deformability was then compared with that of a trapped polystyrene microbead as a function of the applied acoustic pressure for a better understanding of the relationship between the pressure and degree of cell deformation. Cell deformation was found to become more pronounced as higher pressure levels were applied. Furthermore, to determine if this acoustic trapping method can be exploited in quantifying the cell mechanics in a suspension and in a non-contact manner, the deformability levels of breast cancer cells with different degrees of invasiveness due to acoustic trapping were compared. It was found that highly-invasive breast cancer cells exhibited greater deformability than weakly-invasive breast cancer cells. These results clearly demonstrate that the single-beam acoustic trapping technique is a promising tool for non-contact quantitative assessments of the mechanical properties of single cells in suspensions with label-free.
Department of Information and Communication Engineering, Daegu Gyeongbuk Institute of Science &Technology, Daegu, Republic of Korea.
Jae Youn
JY
Hwang
Department of Information and Communication Engineering, Daegu Gyeongbuk Institute of Science &Technology, Daegu, Republic of Korea.
Jihun
J
Kim
Department of Information and Communication Engineering, Daegu Gyeongbuk Institute of Science &Technology, Daegu, Republic of Korea.
Jin Man
JM
Park
NIH Resource Center for Medical Ultrasonic Transducer Technology, Department of Biomedical Engineering, University of Southern California, Los Angeles, CA, USA.
Changyang
C
Lee
NIH Resource Center for Medical Ultrasonic Transducer Technology, Department of Biomedical Engineering, University of Southern California, Los Angeles, CA, USA.
Hayong
H
Jung
Department of Electronic Engineering, Kwangwoon University, Seoul, Republic of Korea.
Jungwoo
J
Lee
NIH Resource Center for Medical Ultrasonic Transducer Technology, Department of Biomedical Engineering, University of Southern California, Los Angeles, CA, USA.
K Kirk
KK
Shung
D001696:Biomechanical Phenomena; D045744:Cell Line, Tumor; D002470:Cell Survival; D006801:Humans; D061986:MCF-7 Cells; D008846:Micromanipulation / Q000295:instrumentation / Q000379:methods; D008863:Microspheres; D010316:Particle Size; D011137:Polystyrenes; D059010:Single-Cell Analysis / Q000295:instrumentation* / Q000379:methods
D011137:Polystyrenes