Global Analysis of the Impact of Deleting Trigger Factor on the Transcriptome Profile of Escherichia coli.
Trigger factor (TF) is a key component of prokaryotic chaperone network, which is involved various basic cellular processes such as nascent peptide folding, protein trafficking, ribosome assembly. To better understanding the physiological roles of TF, global transcriptome profiles of a variety of TF deletion mutant strains of Escherichia coli were determined. We found that deletion of the tig gene, encoding TF, led to a dramatic alteration of transcriptome profile, not only affecting the gene expression of members of the chaperone network, but also changing the levels of quite a few RNAs related to metabolism and other cellular processes. Further studies showed that this alteration was only partially recovered by knockin of TF domain-deletion mutants into the endogenous tig locus, indicating that structural integrity is crucial for the biological function of TF. Finally, by combining the transcriptome and phenotype results, a physiological mechanism underlying the impact of TF deletion on the transcriptome profile was proposed. J. Cell. Biochem. 118: 141-153, 2017. © 2016 Wiley Periodicals, Inc.
School of Life Science and Technology, Harbin Institute of Technology, 2 Yikuang Street, Harbin, 150080, China.|State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Dongjie
D
Fan
Department of Emergency, Beijing Bo'ai Hospital, 10 Jiaomen North Road, Fengtai District, Beijing 100068, China.|China Rehabilitation Research Center, Capital Medical University, Beijing 100068, China.
Lushan
L
Liu
National Research Institute for Family Planning (NRIFP), Beijing 100081, China.
Lingxiang
L
Zhu
China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, China.|Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Wuhan 430072, China.
Fang
F
Peng
School of Life Science and Technology, Harbin Institute of Technology, 2 Yikuang Street, Harbin, 150080, China.|Beijing CapitalBio MedLab, 88 D2, Branch Six Street, Economic and Technological Development Zone, Beijing 101111, China.
Qiming
Q
Zhou
School of Life Science and Technology, Harbin Institute of Technology, 2 Yikuang Street, Harbin, 150080, China.
Chuanpeng
C
Liu
D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D019696:Peptidylprolyl Isomerase / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome / Q000502:physiology*
D029968:Escherichia coli Proteins; C403324:trigger factor, E coli; D019696:Peptidylprolyl Isomerase
CHAPERONE; ESCHERICHIA COLI; TRANSCRIPTOME; TRIGGER FACTOR