Glyco-gold nanoparticle shapes enhance carbohydrate-protein interactions in mammalian cells.
Advances in shape-dependent nanoparticle (NP) research have prompted a close scrutiny of the behaviour of nanostructures in vitro and in vivo. Data pertaining to cellular uptake and site specific sequestration of different shapes of NPs will undoubtedly assist researchers to design better nano-probes for therapeutic and imaging purposes. Herein, we investigated the shape dependent uptake of glyco-gold nanoparticles (G-AuNPs) in different cancer cell lines. Specifically, we have compared the behaviour of spherical, rod and star AuNPs with mannose and galactose conjugations. In vitro experiments showed that the rod-AuNPs exhibited the highest uptake over that of the star and spherical counterparts. Further, an investigation of the mechanism of the uptake clearly demonstrated clathrin mediated endocytosis of the specific G-AuNPs. These results reveal the benefits of different G-AuNP shapes in carbohydrate-mediated interactions.
Indian Institute of Science Education and Research, Pashan, Pune 411008, India. rkikkeri@iiserpune.ac.in.
Sivakoti
S
Sangabathuni
Raghavendra
R
Vasudeva Murthy
Preeti Madhukar
PM
Chaudhary
Manalee
M
Surve
Anirban
A
Banerjee
Raghavendra
R
Kikkeri
D000818:Animals; D004705:Endocytosis; D005690:Galactose / Q000737:chemistry; D006046:Gold; D006367:HeLa Cells; D006801:Humans; D037102:Lectins / Q000737:chemistry; D008358:Mannose / Q000737:chemistry; D053768:Metal Nanoparticles; D051379:Mice; D041681:NIH 3T3 Cells; D011506:Proteins / Q000737:chemistry
D037102:Lectins; D011506:Proteins; D006046:Gold; D008358:Mannose; D005690:Galactose