Development of a Triplet-Triplet Absorption Ruler: DNA- and Chromatin-Mediated Drug Molecule Release from a Nanosurface.
Triplet-triplet (T-T) absorption spectroscopy has been used successfully as a molecular ruler to understand the actual release process of sanguinarine as a drug molecule from a gold nanoparticle surface in the presence of cell components, that is, DNA and chromatin. The obtained results have been verified by fluorescence and surface-enhanced Raman spectroscopy (SERS), and a plausible explanation has been put forward to describe the underestimation and overestimation of the percentage (%) of the release of drug molecules measured by fluorescence- and SERS-based techniques, respectively, over the highlighted T-T absorption spectroscopy. Because of the intrinsic nature of absorption, the reported T-T absorption spectroscopic assay overpowers fluorescence- and SERS-based assays, which are limited by the long-range interaction and nonlinear dependence of the concentration of analytes, respectively.
Sudeshna Das
SD
Chakraborty
Abhishek
A
Sau
Department of Functional Nanosystems and High Temperature Materials, National University of Science and Technology 'MISiS' , Leninsky, Prospect 4, 119049 Moscow, Russia.
Denis V
DV
Kuznetsov
Amrita
A
Banerjee
Munmun
M
Bardhan
Maireyee
M
Bhattacharya
Dipak
D
Dasgupta
Samita
S
Basu
Dulal
D
Senapati
D000818:Animals; D053119:Benzophenanthridines / Q000737:chemistry / Q000378:metabolism; D002417:Cattle; D002843:Chromatin / Q000737:chemistry* / Q000378:metabolism; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D065546:Drug Liberation; D006046:Gold / Q000737:chemistry; D007546:Isoquinolines / Q000737:chemistry / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D004364:Pharmaceutical Preparations / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013059:Spectrum Analysis, Raman
D053119:Benzophenanthridines; D002843:Chromatin; D007546:Isoquinolines; D004364:Pharmaceutical Preparations; D006046:Gold; D004247:DNA; C427203:calf thymus DNA; C005705:sanguinarine
NSET; SERS; Sgr; Triplet−triplet absorption or laser flash photolysis; chromatin; ctDNA; drug