Visualizing Bacillus subtilis During Vegetative Growth and Spore Formation.
Bacillus subtilis is the most commonly used Gram-positive bacterium to study cellular processes because of its genetic tractability. In addition, during nutrient limitation, B. subtilis undergoes the development process of spore formation, which is among the simplest examples of cellular differentiation. Many aspects of these processes have benefited from fluorescence microscopy. Here, we describe basic wide-field fluorescence microscopy techniques to visualize B. subtilis during vegetative growth, and the developmental process of sporulation.
Department of Microbiology & Immunobiology, Harvard Medical School, HIM Building 1018, 77 Ave Louis Pasteur, Boston, MA, 02115, USA. xindan_wang@hms.harvard.edu.
Xindan
X
Wang
Department of Microbiology & Immunobiology, Harvard Medical School, HIM Building 1018, 77 Ave Louis Pasteur, Boston, MA, 02115, USA.
Paula
P
Montero Llopis
D001412:Bacillus subtilis / Q000502:physiology*; D007091:Image Processing, Computer-Assisted; D008856:Microscopy, Fluorescence; D013171:Spores, Bacterial / Q000254:growth & development*
Bacillus subtilis; Fluorescence microscopy; Sporulation; Time-lapse microscopy; Vegetative growth