Surface Plasmon Resonance (SPR) Analysis of Binding Interactions of Inner-Ear Proteins.
Surface plasmon resonance is an optical technique that is utilized for detecting molecular interactions. Binding of a mobile molecule (analyte) to a molecule immobilized on a thin metal film (ligand) changes the refractive index of the film. The angle of extinction of light that is completely reflected after polarized light impinges upon the film, is altered, and monitored as a change in detector position for a dip in reflected intensity (the surface plasmon resonance phenomenon). Because the method strictly detects mass, there is no need to label the interacting components, thus eliminating possible changes of their molecular properties. We have utilized surface plasmon resonance to study interaction of proteins of inner-ear sensory epithelia.
Departments of Otolaryngology and Biochemistry-Molecular Biology, Wayne State University School of Medicine, 540 East Canfield Avenue, Detroit, MI, 48201, USA. ddresche@med.wayne.edu.
Dennis G
DG
Drescher
Department of Otolaryngology, Wayne State University School of Medicine, Detroit, MI, 48201, USA.
Selvakumar
S
Dakshnamurthy
Department of Otolaryngology, Wayne State University School of Medicine, Detroit, MI, 48201, USA.
Marian J
MJ
Drescher
Department of Otolaryngology, Wayne State University School of Medicine, Detroit, MI, 48201, USA.
Neeliyath A
NA
Ramakrishnan
D000818:Animals; D001921:Brain / Q000378:metabolism; D007758:Ear, Inner / Q000378:metabolism*; D051379:Mice; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000379:methods*; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D020349:Surface Plasmon Resonance / Q000379:methods*
D011506:Proteins
Biacore 3000; Fusion protein; Hair cell; Interaction kinetics; Molecular cloning; Protein–protein interaction; Sensor chip CM5; Surface plasmon resonance (SPR)