Comparative analysis of two bacteriophages of Xanthomonas arboricola pv. juglandis.
Walnut blight caused by Xanthomonas arboricola pv. juglandis (Xaj) is one of the most frequent infective diseases of walnut, resulting in serious economic losses. One potential solution to control this disease could be the application of bacteriophages. In this study, 24 phages were isolated from soil and walnut aerial tissues infected with Xaj. Two polyvalent bacteriophages, Xaj2 and Xaj24 were chosen for further characterization including their morphological, physiological and genomic analyses. Xaj2 was classified as Siphoviridae whereas Xaj24 belonged to the Podoviridae family. Both phages demonstrated lytic effect on Xaj in laboratory trials. Complete genomes of Xaj2 and Xaj24 were determined. Genomes of Xaj2 and Xaj24 consisted of 49.241 and 44.861 nucleotides encoding 80 and 53 genes, respectively. Comparative genome analyses have revealed that Xaj2 had a unique genome sequence, while Xaj24 was a phiKMV-like phage and it was most similar to the Prado phage which is virulent for Xylella fastidiosa and Xanthomonas spp. In this study, we present the first two complete Xaj phage sequences enabling an insight into the genomics of Xaj phages.
Department of Biotechnology, Nanophagetherapy Center, Enviroinvest Corporation, Pécs, Hungary.
Dóra
D
Dömötör
Department of Biotechnology, Nanophagetherapy Center, Enviroinvest Corporation, Pécs, Hungary; Faculty of Engineering, University of Pannonia, Veszprém, Hungary.
Tamara
T
Frank
Department of Biotechnology, University of Szeged, Szeged, Hungary; Institute of Biophysics, Biological Research Center, Szeged, Hungary.
Gábor
G
Rákhely
Institute of Biophysics, Biological Research Center, Szeged, Hungary.
Zsolt
Z
Doffkay
Institute of Medical Microbiology and Immunology, University of Pécs, Pécs, Hungary.
György
G
Schneider
Department of Biotechnology, Nanophagetherapy Center, Enviroinvest Corporation, Pécs, Hungary. Electronic address: kovacst@enviroinvest.hu.
Tamás
T
Kovács
D061046:Biological Control Agents; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral; D059014:High-Throughput Nucleotide Sequencing; D031324:Juglans / Q000382:microbiology; D008242:Lysogeny / Q000235:genetics; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology / Q000517:prevention & control; D017902:Podoviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D017906:Siphoviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D012988:Soil Microbiology; D014974:Xanthomonas / Q000254:growth & development / Q000472:pathogenicity / Q000821:virology*
D061046:Biological Control Agents; D004279:DNA, Viral
Bacteriophage; Complete genome sequence; Genomics; Next generation sequencing; Walnut blight; Xanthomonas arboricola pv. juglandis