Extraction of DNA from Human Skeletal Material.
In recent years the recovery and analysis of DNA from skeletal remains has been applied to several contexts ranging from disaster victim identification to the identification of the victims of conflict. Here are described procedures for processing the bone and tooth samples including mechanical and chemical cleaning, cutting and powdering in the presence of liquid nitrogen, complete demineralization of bone and tooth powder, DNA extraction, DNA purification using magnetic beads, and the precautions and strategies implemented to avoid and detect contamination. It has proven highly successful in the analysis of bones and teeth from Second World War victims' skeletal remains that have been excavated from mass graves in Slovenia and is also suitable for genetic identification of relatively fresh human remains.
Laboratory of Molecular Genetics, Institute of Forensic Medicine, Vrazov trg 2, 1104, Ljubljana, Slovenia. irena.zupanic@mf.uni-lj.si.
Irena Zupanič
IZ
Pajnič
D001842:Bone and Bones / Q000737:chemistry*; D016172:DNA Fingerprinting / Q000379:methods*; D018732:Forensic Anthropology / Q000379:methods*; D053803:Forensic Genetics / Q000379:methods*; D006801:Humans; D016133:Polymerase Chain Reaction; D047789:World War II
Bone; Contamination; DNA extraction; Identification; Second World War victims; Teeth