Nelumbo Nucifera leaf protects against UVB-induced wrinkle formation and loss of subcutaneous fat through suppression of MCP3, IL-6 and IL-8 expression.
Nelumbo nucifera has long been used in traditional medicine in East Asian countries such as China and Korea. In this study, we report the different property of several Nelumbo nucifera leaf (NNL) extracts on adipocyte differentiation. Adipogenesis was stimulated by administration of dichloromethyl (DCM) or n-hexan extract of NNL but attenuated by that of water extract. We also show that topical administration of DCM extract of NNL attenuated ultraviolet-B (UVB)-mediated wrinkle formation and reduction of subcutaneous (SC) fat in vivo. Interestingly, UVB-induced blood contents of triglyceride (TG) were attenuated significantly by topical administration of the DCM extract. In addition, we found that UVB-induced expression of cytokines (interleukin-6; IL-6, interleukin-8; IL-8, and monocyte chemotactic protein-3; MCP3), which were reported as regulators in SC fat metabolism, was attenuated in mouse skin fibroblast cells upon administration of the DCM extract. Collectively, our data suggest that topical administration of DCM extract of NNL, which plays a regulatory role in adipogenesis, could attenuate UVB-induced wrinkle formation and the metabolism of blood lipids by regulating the expression of cytokines such as IL-6, IL-8, and MCP3 in skin fibroblast cells. Our findings support further development of DCM extract of NNL as a potential therapeutic agent for prevention of photoaging-related disorders.
Department of Food Science and Biotechnology, Sungkyunkwan University, Seoul, Republic of Korea.
Ki Moon
KM
Park
Department of Food Science and Biotechnology, Sungkyunkwan University, Seoul, Republic of Korea.
Young Ji
YJ
Yoo
Department of Nano-Bioengineering, Incheon National University, 119 Academy-ro, Yeonsu-gu, Incheon 406-772, Republic of Korea.
Sujin
S
Ryu
Department of Nano-Bioengineering, Incheon National University, 119 Academy-ro, Yeonsu-gu, Incheon 406-772, Republic of Korea. Electronic address: seungho@inu.ac.kr.
Seung Ho
SH
Lee
D041721:3T3-L1 Cells; D050156:Adipogenesis / Q000187:drug effects; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D054410:Chemokine CCL7 / Q000235:genetics / Q000378:metabolism; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism; D008518:Medicine, East Asian Traditional; D051379:Mice; D008812:Mice, Hairless; D041681:NIH 3T3 Cells; D031653:Nelumbo / Q000737:chemistry* / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D020011:Protective Agents / Q000737:chemistry* / Q000494:pharmacology; D012867:Skin / Q000473:pathology; D015595:Skin Aging / Q000187:drug effects / Q000528:radiation effects*; D050151:Subcutaneous Fat / Q000502:physiology*; D014280:Triglycerides / Q000097:blood; D014466:Ultraviolet Rays
D054410:Chemokine CCL7; D016207:Cytokines; D005230:Fatty Acids, Nonesterified; D015850:Interleukin-6; D016209:Interleukin-8; D010936:Plant Extracts; D020011:Protective Agents; D014280:Triglycerides
Adipogenesis; Nelumbo nucifera leaf; Subcutaneous fat; UVB; Wrinkle formation