Synthesis and characterization of crosslinked gellan/PVA nanofibers for tissue engineering application.
Electrospun nanofibers based on gellan are considered as promising biomaterial for tissue engineering and wound healing applications. However, major hurdles in usage of these nanofibers are their poor stability and deprived structural consistency in aqueous medium which is a prerequisite for their application in the biomedical sector. In this investigation, three dimensional nanofibers, consisting of gellan and PVA have been fabricated and then stabilized under various crosslinking conditions in order to improve their physiochemical stability. The impacts of different crosslinking procedures on the gellan/PVA nanofibers were examined in terms of changes in morphological, mechanical, swelling and biological properties. Superior tensile strength and strain was recorded in case of crosslinked nanofibers as compared to non-crosslinked nanofibers. Contact angles and swelling properties of fabricated gellan/PVA nanofibers were found to vary with the crosslinking method. All crosslinking conditions were evaluated with regard to their response towards human dermal fibroblast (3T3L1) cells. Biocompatibility studies suggested that the fabricated crosslinked gellan/PVA nanofibers hold a great prospective in the biomedical engineering arena.
Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, Uttarakhand, India. Electronic address: priya.vashisth8@gmail.com.
Priya
P
Vashisth
Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, Uttarakhand, India.
Vikas
V
Pruthi
D001672:Biocompatible Materials / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003432:Cross-Linking Reagents / Q000737:chemistry*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D055595:Mechanical Phenomena; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D011135:Polysaccharides, Bacterial / Q000138:chemical synthesis* / Q000737:chemistry; D011142:Polyvinyl Alcohol / Q000138:chemical synthesis* / Q000737:chemistry; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D011135:Polysaccharides, Bacterial; C048288:gellan gum; D011142:Polyvinyl Alcohol
Biocompatibility; Crosslinking; Gellan; Nanofibers; PVA