Rapid assessment of salivary MMP-8 and periodontal disease using lateral flow immunoassay.
OBJECTIVE
The objective of this study was to determine the efficacy of a novel point-of-care immunoflow device (POCID) for detecting matrix metalloproteinase (MMP)-8 concentrations in oral fluids in comparison with a gold standard laboratory-based immunoassay.


METHODS
Oral rinse fluid and whole expectorated saliva samples were collected from 41 participants clinically classified as periodontally healthy or diseased. Samples were analyzed for MMP-8 by Luminex immunoassay and POCID. Photographed POCID results were assessed by optical scan and visually by two examiners. Data were analyzed by Pearson's correlation and receiver-operating characteristics.


RESULTS
MMP-8 was readily detected by the POCID, and concentrations correlated well with Luminex for both saliva and rinse fluids (r = 0.57-0.93). Thresholds that distinguished periodontitis from health were delineated from both the optical scans and visual reads of the POCID (sensitivity: 0.7-0.9, specificity: 0.5-0.7; P < 0.05).


CONCLUSIONS
Performance of this POCID for detecting MMP-8 in oral rinse fluid or saliva was excellent. These findings help demonstrate the utility of salivary biomarkers for distinguishing periodontal disease from health using a rapid point-of-care approach.
Department of Oral Health Practice, University of Kentucky, Lexington, KY, USA.
N
N
Johnson
Department of Oral Health Practice, University of Kentucky, Lexington, KY, USA.|Center for Oral Health Research, College of Dentistry, University of Kentucky, Lexington, KY, USA.
J L
JL
Ebersole
Department of Biostatistics, College of Public Health, University of Kentucky, Lexington, KY, USA.
R J
RJ
Kryscio
Center for Oral Health Research, College of Dentistry, University of Kentucky, Lexington, KY, USA.
R J
RJ
Danaher
Department of Oral Health Practice, University of Kentucky, Lexington, KY, USA.|Center for Oral Health Research, College of Dentistry, University of Kentucky, Lexington, KY, USA.
D
D
Dawson
Department of Oral Health Practice, University of Kentucky, Lexington, KY, USA.
M
M
Al-Sabbagh
Department of Oral Health Practice, University of Kentucky, Lexington, KY, USA. cmiller@uky.edu.|Center for Oral Health Research, College of Dentistry, University of Kentucky, Lexington, KY, USA. cmiller@uky.edu.
C S
CS
Miller
D000328:Adult; D015415:Biomarkers / Q000032:analysis; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods; D008297:Male; D020784:Matrix Metalloproteinase 8 / Q000032:analysis*; D010510:Periodontal Diseases / Q000201:enzymology*; D010518:Periodontitis / Q000201:enzymology; D019095:Point-of-Care Systems; D012463:Saliva / Q000201:enzymology*
D015415:Biomarkers; D020784:Matrix Metalloproteinase 8
MMP-8; biomarker; periodontitis; point-of-care; saliva