Sevoflurane preconditioning during myocardial ischemia-reperfusion reduces infarct size and preserves autonomic control of circulation in rats.
OBJECTIVE
To investigate the myocardial ischemia-reperfusion with sevoflurane anesthetic preconditioning (APC) would present beneficial effects on autonomic and cardiac function indexes after the acute phase of a myocardial ischemia-reperfusion.


METHODS
Twenty Wistar rats were allocated in three groups: control (CON, n=10), myocardial infarction with sevoflurane (SEV, n=5) and infarcted without sevoflurane (INF, n=5). Myocardial ischemia (60 min) and reperfusion were performed by temporary coronary occlusion. Twenty-one days later, the systolic and diastolic function were evaluated by echocardiography; spectral analysis of the systolic arterial pressure (SAPV) and heart rate variability (HRV) were assessed. After the recording period, the infarct size (IS) was evaluated.


RESULTS
The INF group presented greater cardiac dysfunction and increased sympathetic modulation of the SAPV, as well as decreased alpha index and worse vagal modulation of the HRV. The SEV group exhibited attenuation of the systolic and diastolic dysfunction and preserved vagal modulation (square root of the mean squared differences of successive R-R intervals and high frequency) of HRV, as well as a smaller IS.


CONCLUSIONS
Sevoflurane preconditioning better preserved the cardiac function and autonomic modulation of the heart in post-acute myocardial infarction period.
PhD, Faculdade de Medicina, Universidade de São Paulo (USP), Brazil. Conception and design of the study, analysis of data, manuscript writing., Universidade de São Paulo, Faculdade de Medicina, Universidade de São Paulo (USP), Brazil.
Rubens Campana
RC
Pasqualin
PhD, Hypertension Unit, Experimental Division, Instituto do Coração (InCor), Hospital das Clínicas, Faculdade de Medicina, USP, Sao Paulo-SP, Brazil. Conception of the study, analysis of data, manuscript writing., Universidade de São Paulo, Hospital das Clínicas, Faculdade de Medicina, USP, Sao Paulo SP , Brazil.
Cristiano Teixeira
CT
Mostarda
Graduate student, Hypertension Unit, Experimental Division, Instituto do Coração (InCor), Hospital das Clínicas, Faculdade de Medicina, USP, Sao Paulo-SP, Brazil. Helped conduct the study, analysis of data., Universidade de São Paulo, Hospital das Clínicas, Faculdade de Medicina, USP, Sao Paulo SP , Brazil.
Leandro Ezequiel de
LE
Souza
Postgraduate student in Anesthesiology, Laboratory of Anesthesiology (LIM-08), Faculdade de Medicina, USP, Sao Paulo-SP, Brazil. Helped conduct the study, analysis of data, manuscript writing., Universidade de São Paulo, Laboratory of Anesthesiology (LIM-08), Faculdade de Medicina, USP, Sao Paulo SP , Brazil.
Matheus Fachini
MF
Vane
PhD, Hypertension Unit, Experimental Division, Instituto do Coração (InCor), Hospital das Clínicas, Faculdade de Medicina, USP, Sao Paulo-SP, Brazil. Helped conduct the study, analysis of data., Universidade de São Paulo, Hospital das Clínicas, Faculdade de Medicina, USP, Sao Paulo SP , Brazil.
Raquel
R
Sirvente
PhD, Laboratory of Anesthesiology (LIM-08), Faculdade de Medicina, USP, Sao Paulo-SP, Brazil. Helped conduct the study, analysis of data, manuscript writing., Universidade de São Paulo, Laboratory of Anesthesiology (LIM-08), Faculdade de Medicina, USP, Sao Paulo SP , Brazil.
Denise Aya
DA
Otsuki
Associate Professor, Laboratory of Anesthesiology (LIM-08), Faculdade de Medicina, USP, Sao Paulo-SP, Brazil. Manuscript writing, critical revision., Universidade de São Paulo, Laboratory of Anesthesiology (LIM-08), Faculdade de Medicina, USP, Sao Paulo SP , Brazil.
Marcelo Luís Abramides
ML
Torres
Associate Professor, Hypertension Unit, Experimental Division, Instituto do Coração (InCor), Hospital das Clínicas, USP, Sao Paulo-SP, Brazil. Analysis of data, manuscript writing, critical revision., Universidade de São Paulo, Instituto do Coração (InCor), Hospital das Clínicas, USP, Sao Paulo SP , Brazil.
Maria Cláudia Costa
MC
Irigoyen
Full Professor, Laboratory of Anesthesiology (LIM-08), Faculdade de Medicina, USP, Sao Paulo-SP, Brazil. Analysis of data, manuscript writing, critical revision., Universidade de São Paulo, Laboratory of Anesthesiology (LIM-08), Faculdade de Medicina, USP, Sao Paulo SP , Brazil.
José Otávio Costa
JO
Auler
D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D000818:Animals; D001341:Autonomic Nervous System / Q000187:drug effects* / Q000502:physiology; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology; D004452:Echocardiography; D006339:Heart Rate / Q000187:drug effects / Q000502:physiology; D019157:Ischemic Preconditioning, Myocardial / Q000379:methods*; D008297:Male; D008738:Methyl Ethers / Q000494:pharmacology*; D023421:Models, Animal; D009203:Myocardial Infarction / Q000000981:diagnostic imaging / Q000473:pathology / Q000503:physiopathology* / Q000517:prevention & control; D017202:Myocardial Ischemia / Q000000981:diagnostic imaging / Q000209:etiology / Q000503:physiopathology*; D011674:Pulse; D011897:Random Allocation; D017208:Rats, Wistar; D000077149:Sevoflurane; D013997:Time Factors
D018685:Anesthetics, Inhalation; D008738:Methyl Ethers; D000077149:Sevoflurane