Identification and characteristics of the testes-specific gene, Ccdc38, in mice.
Distinguishing the testes-speciﬁc genes in different species may disclose key genes associated with testes-speciﬁc functions and provide sufficient information for the study and treatment of male infertility. A testes‑specific gene, coiled-coil domain containing 38 (Ccdc38), was identified by screening UniGene libraries. Systematic bioinformatics analysis demonstrated that the CCDC38 protein was conserved in various mammalian species. It was determined that CCDC38 was exclusively expressed in testes and its expression increased from 2‑8 weeks of age. Additional immunohistochemical analysis indicated that CCDC38 was mainly expressed in spermatogonia and spermatocytes. It is of note that, immunofluorescence and co-immunoprecipitation assays demonstrated that CCDC38 interacted with ubiquitinated histone H2A in mouse testes. Therefore, these results suggest that Ccdc38 is a testes-specific gene, which may be important for mouse spermatogenesis.
Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen Peking University‑Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong 518036, P.R. China.
Shou-Ren
SR
Lin
Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen Peking University‑Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong 518036, P.R. China.
Yu-Chi
YC
Li
Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen Peking University‑Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong 518036, P.R. China.
Man-Ling
ML
Luo
Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen Peking University‑Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong 518036, P.R. China.
Huan
H
Guo
Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen Peking University‑Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong 518036, P.R. China.
Tian-Tian
TT
Wang
Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen Peking University‑Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong 518036, P.R. China.
Jian-Bo
JB
Chen
Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen Peking University‑Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong 518036, P.R. China.
Qian
Q
Ma
Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen Peking University‑Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong 518036, P.R. China.
Yan-Li
YL
Gu
Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen Peking University‑Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong 518036, P.R. China.
Zhi-Mao
ZM
Jiang
Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen Peking University‑Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong 518036, P.R. China.
Yao-Ting
YT
Gui
D000818:Animals; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D006657:Histones / Q000378:metabolism; D007150:Immunohistochemistry; D007248:Infertility, Male / Q000235:genetics; D008297:Male; D051379:Mice; D009928:Organ Specificity / Q000235:genetics; D010802:Phylogeny; D011485:Protein Binding; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013090:Spermatocytes / Q000378:metabolism; D013091:Spermatogenesis / Q000235:genetics; D013093:Spermatogonia / Q000378:metabolism; D013737:Testis / Q000378:metabolism*; D014452:Ubiquitins / Q000378:metabolism
D006657:Histones; D012333:RNA, Messenger; D014452:Ubiquitins; C021645:chromatin conjugate protein A24