Effect of Factors Secreted by the Placenta on Phenotype of THP-1 Cells Cultured on a 3D Scaffold.
We studied the effects of secretory products of the placenta obtained from women with normal pregnancy and preeclampsia on the expression of surface markers by THP-1 cells cultured on a 3D Matrigel scaffold. Secretory products of third trimester placentas obtained from women with normal pregnancy reduced the relative number of THP-1 cells expressing CD54 and CD14 molecules and expression of CD14 and CD95 molecules by THP-1 cells in comparison with the effect of secretory products first trimester placentas. In parallel, the intensity of CD49d expression by THP-1 cells increased in the presence of secretory products of third trimester placentas in comparison with the first trimester. No differences in the expression of the studied molecules by THP-1 cells under the effect of placentas from women with physiological pregnancy and patients with preeclampsia were found.
D. O. Ott Reseach Institute of Obstetrics and Gynecology, St. Petersburg, Russia.
T Yu
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Lvova
D. O. Ott Reseach Institute of Obstetrics and Gynecology, St. Petersburg, Russia.
O I
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Stepanova
D. O. Ott Reseach Institute of Obstetrics and Gynecology, St. Petersburg, Russia.
L P
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Viazmina
D. O. Ott Reseach Institute of Obstetrics and Gynecology, St. Petersburg, Russia.
L S
LS
Okorokova
D. O. Ott Reseach Institute of Obstetrics and Gynecology, St. Petersburg, Russia.
K L
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Belyakova
D. O. Ott Reseach Institute of Obstetrics and Gynecology, St. Petersburg, Russia.
M E
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Belikova
D. O. Ott Reseach Institute of Obstetrics and Gynecology, St. Petersburg, Russia.
S A
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Selkov
D. O. Ott Reseach Institute of Obstetrics and Gynecology, St. Petersburg, Russia. falcojugger@yandex.ru.
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Sokolov
D015703:Antigens, CD / Q000378:metabolism; D018929:Cell Culture Techniques; D002460:Cell Line; D005260:Female; D006801:Humans; D008264:Macrophages / Q000378:metabolism*; D010641:Phenotype; D010920:Placenta / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D011263:Pregnancy Trimester, Third
D015703:Antigens, CD
macrophages; placenta; preeclampsia; surface receptors