Cryopreservation of white-tailed deer (Odocoileus virginianus) semen using soybean-, liposome-, and egg yolk-based extenders.
The objectives of the present study were to compare the use of soybean-based (Andromed), liposome-based (Optixcell), and egg yolk-based (Ovine Red, Triladyl, and Biladyl) extenders for cryopreservation of white-tailed deer semen. In experiment 1, ejaculates obtained from six bucks were aliquoted into the following extenders: Andromed, Ovine Red, Triladyl, and Biladyl (containing 4%, 6%, or 8% of glycerol). In experiment 2, ejaculates obtained from eight bucks were divided amongst Andromed, Ovine Red, and Optixcell extenders. Total and progressive sperm motility were assessed for each sample before and after cryopreservation using a computer-automated semen analyzer. In experiment 2, flow cytometry was used for post-thaw assessment of sperm viability (SYBR-14/PI), acrosome integrity (FITC-PNA/PI), and chromatin stability (acridine orange). In experiment 1, both Andromed and Ovine Red extenders exhibited higher post-thaw total motility than Biladyl containing 4% or 6% of glycerol (p<0.05). Andromed also produced higher progressive motility than all other extenders (p<0.01) before and after cryopreservation with no differences amongst the other extenders (p≥0.11). In experiment 2, there were no differences in total and progressive motility between Andromed, Ovine Red, or Optixcell extenders (p≥0.39). Additionally, there were no differences in sperm viability (p=0.18), acrosome integrity in viable sperm (p≥0.10), or DNA fragmentation index (p=0.15). These results demonstrated that soybean (Andromed) and liposome-based (Optixcell) extenders are equally as effective at cryopreserving white-tailed semen as egg yolk-based Ovine Red extender, but are superior to egg yolk-based Biladyl or Triladyl extenders.
Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana-Champaign, Urbana, IL 61802, USA.
Jamie L
JL
Stewart
Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana-Champaign, Urbana, IL 61802, USA. Electronic address: cshipley@illinois.edu.
Clifford F
CF
Shipley
Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana-Champaign, Urbana, IL 61802, USA.
Ashley Seder
AS
Katich
Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana-Champaign, Urbana, IL 61802, USA.
Eleonora
E
Po
Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana-Champaign, Urbana, IL 61802, USA.
Robyn E
RE
Ellerbrock
Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana-Champaign, Urbana, IL 61802, USA.
Fabio S
FS
Lima
Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana-Champaign, Urbana, IL 61802, USA. Electronic address: canisso@illinois.edu.
Igor F
IF
Canisso
D000818:Animals; D015925:Cryopreservation / Q000662:veterinary*; D003451:Cryoprotective Agents / Q000737:chemistry / Q000494:pharmacology; D003670:Deer / Q000502:physiology*; D004530:Egg Yolk / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D008297:Male; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013025:Glycine max / Q000737:chemistry*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects / Q000502:physiology
D003451:Cryoprotective Agents; D008081:Liposomes
Cervid andrology; Fluorescent probes; Semen biotechnology; Semen evaluation; Semen freezing