Synthesis and characterization of glucosyl stevioside using Leuconostoc dextransucrase.
Glucosyl stevioside was synthesized via transglucosylation by dextransucrase from Leuconostoc citreum KM20 (LcDexT), forming α-d-glucosyl stevioside. A production yield of 94% was reached after 5days of LcDexT reaction at 30°C. Glucosyl stevioside induced a 2-fold improved quality of taste and sweetness, compared to stevioside. After 15days of storage at 25°C, 98% of glucosyl stevioside in an aqueous solution was present in a soluble form, compared to only 11% for stevioside or rebaudioside A. Furthermore, glucosyl stevioside exhibited a similar or improved stability in commercially available soft drinks, when compared to stevioside and rebaudioside A. These results suggest that glucosyl stevioside could serve as a highly pure and stable sweetener in soft drinks.
Eco-Friendly Bio-material Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 56212, Republic of Korea. Electronic address: jinako@kribb.re.kr.
Jin-A
JA
Ko
Department of Food Science & Technology and BK21 Plus Program, and Bio-energy Research Center, Chonnam National University, Gwangju 61186, Republic of Korea. Electronic address: namsh1000@daum.net.
Seung-Hee
SH
Nam
Eco-Friendly Bio-material Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 56212, Republic of Korea. Electronic address: loveme@kribb.re.kr.
Ji-Young
JY
Park
Department of Food Science and Technology, Yeungnam University, Gyeongsan, Gyeongbuk 38541, Republic of Korea. Electronic address: yjwee@ynu.ac.kr.
YongJung
Y
Wee
Research Institute of Food Industrialization, Institute of Green Bio Science & Technology, Seoul National University, Pyeongchang 25354, Republic of Korea. Electronic address: kimdm@snu.ac.kr.
Doman
D
Kim
Eco-Friendly Bio-material Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 56212, Republic of Korea. Electronic address: wslee@kribb.re.kr.
Woo Song
WS
Lee
Eco-Friendly Bio-material Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 56212, Republic of Korea. Electronic address: u9897854@jnu.ac.kr.
Young Bae
YB
Ryu
Department of Food Science & Technology and BK21 Plus Program, and Bio-energy Research Center, Chonnam National University, Gwangju 61186, Republic of Korea. Electronic address: ybryu@kribb.re.kr.
Young-Min
YM
Kim
D002253:Carbonated Beverages; D045786:Diterpenes, Kaurane / Q000138:chemical synthesis*; D005503:Food Additives / Q000138:chemical synthesis; D005960:Glucosides / Q000138:chemical synthesis*; D005964:Glucosyltransferases / Q000138:chemical synthesis* / Q000302:isolation & purification; D006801:Humans; D007934:Leuconostoc / Q000201:enzymology*; D013549:Sweetening Agents / Q000138:chemical synthesis*; D055697:Taste Perception / Q000502:physiology
D045786:Diterpenes, Kaurane; D005503:Food Additives; D005960:Glucosides; D013549:Sweetening Agents; C012043:stevioside; D005964:Glucosyltransferases; C020025:dextransucrase
Dextransucrase; Leuconostoc citreum; Rebaudioside A; Stevioside