Transcriptional Profiling and miRNA-Target Network Analysis Identify Potential Biomarkers for Efficacy Evaluation of Fuzheng-Huayu Formula-Treated Hepatitis B Caused Liver Cirrhosis.
Fuzheng-Huayu (FZHY) formula has been found to have a satisfactory effect on hepatitis B-caused cirrhosis (HBC) treatment. However, the efficacy evaluation of FZHY is often challenging. In this study, a randomized, double-blind and placebo-controlled trial was used to evaluate the therapeutic efficacy of FZHY in HBC treatment. In the trial, 35 medical indexes were detected, and 14 indexes had a statistically-significant difference before compared to after the trial. Importantly, the Child-Pugh score also demonstrated FZHY having therapeutic efficacy. Furthermore, the microRNA (miRNA) profiles of 12 serum samples were detected in FZHY groups, and 112 differential-expressed (DE) miRNAs were determined. Using predicted miRNA targets, 13 kernel miRNAs were identified from the established miRNA-target network. Subsequently, quantitative Real-time Polymerase Chain Reaction (qRT-PCR) was used to validate the expression level of 13 identified miRNAs in the trials. The results showed that nine miRNAs have a statistically-significant difference before compared to after FZHY treatment. By means of a logistic regression model, a miRNA panel with hsa-miR-18a-5p, -326, -1182 and -193b-5p was established, and it can clearly improve the accuracy of the efficacy evaluation of FZHY. This study suggested that the particular miRNAs can act as potential biomarkers and obviously increase the diagnostic accuracy for drug evaluation in HBC treatment progression.
Research Center for Traditional Chinese Medicine Complexity System, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. cqlw1975@126.com.|Laboratory of Epigenetics, Institute of Biomedical Science, Fudan University, Shanghai 200032, China. cqlw1975@126.com.
Qilong
Q
Chen
Laboratory of Epigenetics, Institute of Biomedical Science, Fudan University, Shanghai 200032, China. qyp2015@126.com.
Feizhen
F
Wu
Department of Endocrinology and Metabolism, Shanghai First People's Hospital, Shanghai Jiaotong University, Shanghai 201201, China. cql0218@163.com.
Mei
M
Wang
Research Center for Traditional Chinese Medicine Complexity System, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. wenli228@163.com.
Shu
S
Dong
Research Center for Traditional Chinese Medicine Complexity System, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. cqlw0218@163.com.
Yamin
Y
Liu
Research Center for Traditional Chinese Medicine Complexity System, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. ava0048@163.com.
Yiyu
Y
Lu
Research Center for Traditional Chinese Medicine Complexity System, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. ynsong@163.com.
Yanan
Y
Song
Research Center for Traditional Chinese Medicine Complexity System, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. tazhou@163.com.
Qianmei
Q
Zhou
Institute of Liver Disease, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. liupingg1@126.com.
Ping
P
Liu
Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. yqluo09@sohu.com.
Yunquan
Y
Luo
Research Center for Traditional Chinese Medicine Complexity System, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. shibing07@163.com.
Shibing
S
Su
D015415:Biomarkers; D016000:Cluster Analysis; D019295:Computational Biology / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D006509:Hepatitis B / Q000150:complications*; D006801:Humans; D008103:Liver Cirrhosis / Q000188:drug therapy* / Q000209:etiology* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics*; D034622:RNA Interference; D012372:ROC Curve; D059467:Transcriptome; D016896:Treatment Outcome
D015415:Biomarkers; D004365:Drugs, Chinese Herbal; D035683:MicroRNAs; C532829:fuzheng huayu
Fuzheng-Huayu (FZHY) formula; hepatitis B-caused cirrhosis (HBC); miRNA-target network; microRNA