Anti-oxidative feedback and biomarkers in the intertidal seagrass Zostera japonica induced by exposure to copper, lead and cadmium.
To investigate the potential influences of anthropogenic pollutants, we evaluated the responses of the intertidal seagrass Zostera japonica to three heavy metals: copper (Cu), lead (Pb), and cadmium (Cd). Z. japonica was exposed to various concentrations of Cu, Pb, and Cd (0, 0.5, 5, 50μM) over seven days. The effects were then analyzed using the antioxidant enzyme catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), glutathione peroxidase (GPX), and lipid peroxidation measured using malondialdehyde (MDA) as proxy. Metal accumulation in the above-ground tissues and phenotypic changes were also investigated. Our results revealed that heavy metal concentration increased in seagrass exposed to high levels of metals. Z. japonica has great potential for metal accumulation and a suitable candidate for the decontamination of moderately Cu contaminated bodies of water and can also potentially enhanced efforts of environmental decontamination, either through phytoextraction abilities or by functioning as an indicator for monitoring programs that use SOD, CAT, GPX, POD and MDA as biomarkers.
State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, Beijing 100875, PR China.
Haiying
H
Lin
State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, Beijing 100875, PR China. Electronic address: suntao@bnu.edu.cn.
Tao
T
Sun
Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China. Electronic address: yizhou@qdio.ac.cn.
Yi
Y
Zhou
Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China.
Xiaomei
X
Zhang
D000975:Antioxidants; D015415:Biomarkers / Q000378:metabolism; D002104:Cadmium / Q000032:analysis / Q000633:toxicity; D002374:Catalase / Q000378:metabolism; D003300:Copper / Q000032:analysis / Q000633:toxicity; D004784:Environmental Monitoring / Q000379:methods*; D005979:Glutathione Peroxidase / Q000378:metabolism; D007854:Lead / Q000032:analysis / Q000633:toxicity; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D019216:Metals, Heavy / Q000032:analysis / Q000633:toxicity*; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000378:metabolism; D035962:Zosteraceae / Q000502:physiology*
D000975:Antioxidants; D015415:Biomarkers; D019216:Metals, Heavy; D002104:Cadmium; D007854:Lead; D008315:Malondialdehyde; D003300:Copper; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
Antioxidant response; Biomarker; Heavy metals; Seagrass; Zostera japonica