Emticicia paludis sp. nov., isolated from wetland freshwater.
A novel bacterium, designated HMF3850T, was isolated from freshwater collected from the Gyeong-an wetland, Republic of Korea. Cells were Gram-stain-negative, aerobic, straight rods, non-motile, and oxidase- and catalase-positive. Growth was observed at pH 6.0-8.0 and at 10-30 °C on R2A agar. Comparative analysis of 16S rRNA gene sequences revealed that strain HMF3850T is a member of the genus Emticicia, sharing highest sequence similarities with Emticicia aquatica HMF2925T (96.7 % 16S rRNA gene sequence similarity), Emticicia sediminis JBR12T (95.0 %), Emticicia ginsengisoliGsoil 085T (94.9 %) and Emticicia oligotrophica DSM 17448T (94.7 %). The major fatty acids were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0. The predominant respiratory quinone was MK-7. The major polar lipids were phosphatidylethanolamine, phosphatidylinositol, one unidentified aminophospholipid, two unidentified aminolipids and eight unidentified polar lipids. The DNA G+C content was 38.5 mol %. On the basis of the evidence presented in this study, strain HMF3850T represents a novel species of the genus Emticicia, for which the name Emticicia paludis sp. nov. is proposed. The type strain is HMF3850T (=KCTC 42851T=CECT 9087T).
Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, Gyeonggi 17035, Republic of Korea.
Heeyoung
H
Kang
Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, Gyeonggi 17035, Republic of Korea.
Haneul
H
Kim
Department of Biological Sciences, Inha University, Incheon 22212, Republic of Korea.
Yochan
Y
Joung
Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, Gyeonggi 17035, Republic of Korea.
Kiseong
K
Joh
D015373:Bacterial Typing Techniques; D001482:Base Composition; D041964:Cytophagaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005618:Fresh Water / Q000382:microbiology*; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry; D053833:Wetlands
D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7