The efficacy of Na-butyrate encapsulated in palm fat on performance of broilers infected with necrotic enteritis with gene expression analysis.
OBJECTIVE
To study the efficacy of Na-butyrate encapsulated in palm fat on performance of broiler chickens experimentally infected with necrotic enteritis (NE) with the determination of its protective effect against the changes in the gene expression profiles and deoxyribonucleic acid (DNA) fragmentation.


METHODS
A total of 800 one-day-old male Arbor Acres Plus broiler chickens were randomly allocated into four groups for 5 weeks. Na-butyrate was supplemented at dosages of 1 kg/ton for starter diet, 0.5 kg/ton for grower diet, and 0.25 kg/ton for finisher diet (presence or absence). Birds of groups 1 and 2 were inoculated by crop gavages with 4×10(8) CFU/ml/bird of Clostridium perfringens in phosphate buffered saline for 4 successive days, from 14 to 17 days of age to produce NE.


RESULTS
Addition of Na-butyrate, encapsulated in palm fat, to ration of experimentally infected broilers with NE resulted in increased final body weight, at 35 days of age, reduced total feed consumption, improved feed conversion ratio, reduced cumulative mortality, and increased production number. There were increased intestinal diameter, intestinal length, and significantly increased the weight of bursa of Fabricius(BF) with higher hemagglutination inhibition titers against Newcastle disease (ND) vaccination versus untreated infected and untreated negative control birds. The results showed increased expression levels of alpha-toxin and glyceraldehyde-3-phosphate dehydrogenase in the bursa tissues of broilers infected with C. perfringens. However, the expression levels of these genes in broilers treated with Na-butyrate were similar to the non-infected control group. Supplementation of broilers with Na-butyrate increased the expression level of insulin-like growth factor-1 (IGF-1) and decreased the DNA fragmentation induced by C. perfringens.


CONCLUSIONS
Na-butyrate significantly improved chicken broiler body weights, increased relative weights of BF, increased antibody titers against ND vaccination, numerically lowered mortality due to C. perfringens infection, increased the expression level of IGF-1, and decreased the DNA fragmentation induced by C. perfringens. Obtained results point out the effectiveness of Na-butyrate encapsulated in palm fat in improving the production performance variables, immune response, and intestinal morphology in experimentally induced NE as well as in non-infected chicken broilers.
Department of Cell Biology, National Research Centre, Dokki, Cairo, Egypt.
M G
MG
Eshak
Department of Animal Production, Faculty of Agriculture, Cairo University, Cairo, Egypt.
M A
MA
Elmenawey
Department of Animal Production, Faculty of Agriculture, Cairo University, Cairo, Egypt.
A
A
Atta
Department of Animal Production, Faculty of Agriculture, Cairo University, Cairo, Egypt.
H B
HB
Gharib
Department of Bacteriology, Animal Health Research Institute, Dokki, Cairo, Egypt.
B
B
Shalaby
Department of Poultry Diseases, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt.
M H H
MH
Awaad
Na-butyrate; chickens; deoxyribonucleic acid fragmentation; gene expression analysis; necrotic enteritis