Oxidative stress mediates dibutyl phthalateinduced anxiety-like behavior in Kunming mice.
Among all phthalate esters, dibutyl phthalate (DBP) is only second to di-(2-ethylhexyl) phthalate (DEHP) in terms of adverse health outcomes, and its potential cerebral neurotoxicity has raised concern in recent years. DBP exposure has been reported to be responsible for neurobehavioral effects and related neurological diseases. In this study, we found that neurobehavioral changes induced by DBP may be mediated by oxidative damage in the mouse brain, and that the co-administration of Mangiferin (MAG, 50mg/kg/day) may protect the brain against oxidative damage caused by DBP exposure. The results of ethological analysis (elevated plus maze test and open-field test), histopathological examination of the brain, and assessments of oxidative stress (OS) in the mouse brain showed that there is a link between oxidative stress and anxiety-like behavior produced by DBP at higher doses (25 or 125mg/kg/day). Biomarkers of oxidative stress encompass reactive oxygen species (ROS), glutathione (GSH), malondialdehyde (MDA) and DPC coefficients (DPC). MAG (50mg/kg/day),administered as an antioxidant,can attenuatetheanxiety-like behavior of the tested mice.
College of Basic Medical Science, Hubei University of Science and Technology, Xianning 437100, China.
Biao
B
Yan
Laboratory of Environmental Biomedicine, School of Life Sciences, Central China Normal University, Wuhan 430079, China.
Junhui
J
Guo
Laboratory of Environmental Biomedicine, School of Life Sciences, Central China Normal University, Wuhan 430079, China.
Xudong
X
Liu
Laboratory of Environmental Biomedicine, School of Life Sciences, Central China Normal University, Wuhan 430079, China.
Jinquan
J
Li
College of Basic Medical Science, Hubei University of Science and Technology, Xianning 437100, China; Laboratory of Environmental Biomedicine, School of Life Sciences, Central China Normal University, Wuhan 430079, China.
Xu
X
Yang
College of Basic Medical Science, Hubei University of Science and Technology, Xianning 437100, China. Electronic address: mping68@126.com.
Ping
P
Ma
College of Basic Medical Science, Hubei University of Science and Technology, Xianning 437100, China. Electronic address: wysj2007@126.com.
Yang
Y
Wu
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001007:Anxiety / Q000139:chemically induced* / Q000473:pathology; D001522:Behavior, Animal / Q000187:drug effects; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D003993:Dibutyl Phthalate / Q000633:toxicity*; D005978:Glutathione / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D044004:Xanthones / Q000494:pharmacology
D000975:Antioxidants; D017382:Reactive Oxygen Species; D044004:Xanthones; C013592:mangiferin; D003993:Dibutyl Phthalate; D008315:Malondialdehyde; D005978:Glutathione
Anxiety-like behavior; Dibutyl phthalate (DBP); Mangiferin (MAG); Mice; Oxidative stress (OS)