Binding properties of ruthenium(II) complexes [Ru(bpy)2(ppn)](2+) and [Ru(phen)2(ppn)](2+) with triplex RNA: As molecular "light switches" and stabilizers for poly(U)·poly(A)*poly(U) triplex.
Stable RNA triplexes play key roles in many biological processes, while triplexes are thermodynamically less stable than the corresponding duplexes due to the Hoogsteen base pairing. To understand the factors affecting the stabilization of RNA triplexes by octahedral ruthenium(II) complexes, the binding of [Ru(bpy)2(ppn)](2+) (1, bpy=2,2'-bipyridine, ppn=2,4-diaminopyrimido[5,6-b]dipyrido[2,3-f:2',3'-h]quinoxaline) and [Ru(phen)2(ppn)](2+) (2, phen=1,10-phenanthroline) to poly(U)·poly(A)*poly(U) (· denotes the Watson-Crick base pairing and * denotes the Hoogsteen base pairing) has been investigated. The main results obtained here suggest that complexes 1 and 2 can serve as molecular "light switches" and stabilizers for poly(U)·poly(A)*poly(U), while the effectiveness of complex 2 are more marked, suggesting that the hydrophobicity of ancillary ligands has a significant effect on the two Ru(II) complexes binding to poly(U)·poly(A)*poly(U). This study further advances our knowledge on the binding of RNA triplexes with metal complexes, particularly with octahedral ruthenium polypyridyl complexes.
College of Chemistry, Xiangtan University, Xiangtan 411105, PR China.
Jia
J
Li
College of Chemistry, Xiangtan University, Xiangtan 411105, PR China.
Yanmei
Y
Sun
College of Chemistry, Xiangtan University, Xiangtan 411105, PR China.
Zhiyuan
Z
Zhu
College of Chemistry, Xiangtan University, Xiangtan 411105, PR China.
Hong
H
Zhao
College of Chemistry, Xiangtan University, Xiangtan 411105, PR China; Key Lab of Environmentally Friendly Chemistry and Application in Ministry of Education, Xiangtan University, Xiangtan 411105, PR China. Electronic address: lfwyxh@yeah.net.
Lifeng
L
Tan
D011061:Poly A / Q000737:chemistry*; D011072:Poly U / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D020871:RNA Stability; D012428:Ruthenium / Q000737:chemistry*
D011061:Poly A; D011072:Poly U; D012313:RNA; D012428:Ruthenium
Molecular light switch; Ruthenium(II) complexes; Stabilizer; Triplex RNA