Therapeutic effect of Pseudomonas aeruginosa phage YH30 on mink hemorrhagic pneumonia.
Hemorrhagic pneumonia caused by Pseudomonas aeruginosa remains one of the most costly infectious diseases among farmed mink and commonly leads to large economic losses during mink production. The objective of this study was to investigate the potential of using phages as a therapy against hemorrhagic pneumonia in mink. A broad-host-range phage from the Podoviridae family, YH30, was isolated using the mink-originating P. aeruginosa (serotype G) D7 strain as a host. The genome of YH30 was 72,192bp (54.92% G+C), contained 86 open reading frames and lacked regions encoding known virulence factors, integration-related proteins or antibiotic resistance determinants. These characteristics make YH30 eligible for use in phage therapy. The results of a curative treatment experiment demonstrated that a single intranasal administration of YH30 was sufficient to cure hemorrhagic pneumonia in mink. The mean colony count of P. aeruginosa in the blood and lung of YH30-protected mink was less than 10(3) CFU/mL (g) within 24h of bacterial challenge and ultimately became undetectable, whereas that in unprotected mink reached more than 10(8) CFU/mL (g). Additionally, YH30 dramatically improved the pathological manifestations of lung injury in mink with hemorrhagic pneumonia. Our work demonstrates the potential of phages to treat P. aeruginosa-caused hemorrhagic pneumonia in mink.
College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
Jingmin
J
Gu
College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
Xinwei
X
Li
College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
Mei
M
Yang
College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
Chongtao
C
Du
College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
Ziyin
Z
Cui
College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
Pengjuan
P
Gong
College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
Feifei
F
Xia
College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
Jun
J
Song
College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
Lei
L
Zhang
College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
Juecheng
J
Li
College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
Chuang
C
Yu
College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
Changjiang
C
Sun
College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
Xin
X
Feng
College of Veterinary Medicine, Jilin University, Changchun 130062, PR China.
Liancheng
L
Lei
College of Veterinary Medicine, Jilin University, Changchun 130062, PR China; Jiangsu Co-innovation Center for the Prevention and Control of important Animal Infectious Disease and Zoonoses, Yangzhou 225009, PR China. Electronic address: hanwy@jlu.edu.cn.
Wenyu
W
Han
D000281:Administration, Intranasal; D000818:Animals; D058491:Bacterial Load; D001691:Biological Therapy / Q000592:standards / Q000662:veterinary*; D016679:Genome, Viral / Q000235:genetics; D046529:Microscopy, Electron, Transmission; D008907:Mink; D018410:Pneumonia, Bacterial / Q000628:therapy / Q000662:veterinary*; D011552:Pseudomonas Infections / Q000628:therapy / Q000662:veterinary*; D017105:Pseudomonas Phages / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology* / Q000648:ultrastructure; D011550:Pseudomonas aeruginosa / Q000502:physiology / Q000821:virology*; D016896:Treatment Outcome
Bacteriophage; Mink; Pneumonia; Pseudomonas aeruginosa