Defect in ND2, COX2, ATP6 and COX3 mitochondrial genes as a risk factor for canine mammary tumour.
The aim of this study was to identify mutations in ND2, COX2, ATP6 and COX3 mitochondrial genes in canine mammary tumour, determine their association with the process of neoplastic transformation, and phenotypic traits of dogs. In total, 93 biological samples, including blood, normal and neoplastic tissue samples from 31 dogs with diagnosed malignant canine mammary tumours were analysed. DNA sequencing of genes as well as bioinformatics and statistical analyses were performed. A total of 28 polymorphic loci and 11 mutations were identified. One of the mutations was blood heteroplasmy and two of the mutations caused an amino acid change in p.N117S and p.A184T. For the first time, mutations in mitochondrial genes were detected in dogs with mammary tumours. A statistically significant association between the presence of mutations and the size and age of dogs was demonstrated. Some of these changes may imply that these are the hotspot mutations of canine mammary tumour.
Department of Biological Bases of Animal Production, University of Life Sciences in Lublin, Lublin, Poland.
M
M
Surdyka
Department of Biological Bases of Animal Production, University of Life Sciences in Lublin, Lublin, Poland.
B
B
Slaska
D000818:Animals; D051546:Cyclooxygenase 2 / Q000235:genetics*; D004283:Dog Diseases / Q000235:genetics*; D004285:Dogs; D003576:Electron Transport Complex IV / Q000235:genetics*; D005260:Female; D050259:Genes, Mitochondrial / Q000235:genetics*; D020022:Genetic Predisposition to Disease / Q000235:genetics; D015674:Mammary Neoplasms, Animal / Q000235:genetics*; D025261:Mitochondrial Proton-Translocating ATPases / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D009245:NADH Dehydrogenase / Q000235:genetics*; D012307:Risk Factors
D051546:Cyclooxygenase 2; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV; D025261:Mitochondrial Proton-Translocating ATPases
dog; genes; heteroplasmy; mtDNA; mutations; tumour