Gradient domestication of Haematococcus pluvialis mutant with 15% CO2 to promote biomass growth and astaxanthin yield.
In order to increase biomass yield and reduce culture cost of Haematococcus pluvialis with flue gas from coal-fired power plants, a screened mutant by nuclear irradiation was gradually domesticated with 15% CO2 to promote biomass dry weight and astaxanthin yield. The biomass yield of mutant after 10 generations of 15% CO2 domestication increased to 1.3 times as that with air. With the optimization of nitrogen and phosphorus concentration, the biomass dry weight was further increased by 62%. The astaxanthin yield induced with 15% CO2 and high light of 135 μmol photons m(-2) s(-1) increased to 87.4mg/L, which was 6 times higher than that induced with high light in air.
State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China. Electronic address: juncheng@zju.edu.cn.
Jun
J
Cheng
State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
Ke
K
Li
State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
Zongbo
Z
Yang
State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
Hongxiang
H
Lu
State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
Junhu
J
Zhou
State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
Kefa
K
Cen
D000388:Air; D018533:Biomass; D001709:Biotechnology / Q000379:methods; D002245:Carbon Dioxide / Q000378:metabolism*; D000460:Chlorophyta / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D000071278:Domestication; D008027:Light; D009154:Mutation; D009584:Nitrogen / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism; D024341:Xanthophylls / Q000096:biosynthesis
D024341:Xanthophylls; D002245:Carbon Dioxide; D010758:Phosphorus; C005948:astaxanthine; D009584:Nitrogen
15% CO(2) domestication; Astaxanthin; Biomass growth; Haematococcus pluvialis mutant