Homoeologue expression insights into the basis of growth heterosis at the intersection of ploidy and hybridity in Cyprinidae.
Hybridization and polyploidization are considered important driving forces that form new epigenetic regulations. To study the changing patterns of expression accompanying hybridization and polyploidization, we used RNA-seq and qRT-PCR to investigate global expression and homoeologue expression in diploid and tetraploid hybrids of Carassius auratus red var. (♀) (R) and Cyprinus carpio (♂) (C). By comparing the relative expression levels between the hybrids and their parents, we defined the expression level dominance (ELD) and homoeologue expression bias (HEB) in liver tissue. The results showed that polyploidization contributed to the conversion of homoeologue ELD. In addition, hybridization had more effect on the change in HEB than polyploidization, while polyploidization had more effect on the change of global gene expression than hybridization. Meanwhile, similar expression patterns were found in growth-related genes. The results suggested that hybridization and polyploidization result in differential degrees of maternal HEB in three tissues (liver, muscle and ovary) tested. The results of this study will increase our understanding of the underlying regulation mechanism of rapid growth in diploid hybrids and allotetraploids. The differential degrees of global expression and homoeologue expression contribute to growth heterosis in newly formed hybrids, ensuring the on-going success of allotetraploid speciation.
Key Laboratory of Protein Chemistry and Fish Developmental Biology of Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China.
Li
L
Ren
Key Laboratory of Protein Chemistry and Fish Developmental Biology of Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China.
Wuhui
W
Li
Key Laboratory of Protein Chemistry and Fish Developmental Biology of Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China.
Min
M
Tao
Key Laboratory of Protein Chemistry and Fish Developmental Biology of Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China.
Qinbo
Q
Qin
State Key Laboratory for Conservation and Utilization of Bio-resource, Yunnan University, Kunming, 650091, Yunnan,China.
Jing
J
Luo
State Key Laboratory for Conservation and Utilization of Bio-resource, Yunnan University, Kunming, 650091, Yunnan,China.
Jing
J
Chai
Key Laboratory of Protein Chemistry and Fish Developmental Biology of Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China.
Chenchen
C
Tang
Key Laboratory of Protein Chemistry and Fish Developmental Biology of Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China.
Jun
J
Xiao
Key Laboratory of Protein Chemistry and Fish Developmental Biology of Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China.
Xiaojun
X
Tang
State Key Laboratory for Conservation and Utilization of Bio-resource, Yunnan University, Kunming, 650091, Yunnan,China.
Guoliang
G
Lin
Key Laboratory of Protein Chemistry and Fish Developmental Biology of Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China.
Chun
C
Zhang
Key Laboratory of Protein Chemistry and Fish Developmental Biology of Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China.
Wei
W
Duan
Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore.
Yunhan
Y
Hong
Key Laboratory of Protein Chemistry and Fish Developmental Biology of Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China.
Shaojun
S
Liu
D000818:Animals; D002347:Carps / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D005260:Female; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism; D049810:Genetic Speciation; D006054:Goldfish / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D006823:Hybrid Vigor; D006824:Hybridization, Genetic; D008099:Liver / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism; D009928:Organ Specificity; D010053:Ovary / Q000378:metabolism; D011123:Polyploidy; D017385:Sequence Homology; D059467:Transcriptome
D029941:Fish Proteins