Aluminum Trichloride Inhibits the Rat Osteoblasts Mineralization In Vitro.
Aluminum (Al) is an accumulative toxic metal. Excessive Al accumulation inhibits osteoblasts mineralization and induces osteoporosis. However, the inhibition mechanism of Al on the mineralization is not fully understood. Thus, in this study, the rat osteoblasts were cultured and exposed to 0 mmol L-1(control group, CG) and 0.52 mmol L-1aluminum trichloride (AlCl3, treatment group, TG) for 7, 14, and 21 days, respectively. We found that mineralized matrix nodules, the activity of bone alkaline phosphatase, the concentration of extracellular calcium, the mRNA expression of type-I collagen, the mRNA and protein expressions of osteopontin, osteocalcin, and bone sialoprotein were all decreased, while the concentration of extracellular phosphorus was increased in TG compared with CG with time prolonged. Taken together, these results indicated that AlCl3inhibited osteoblasts mineralization in vitro.
College of Veterinary Medicine, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, China.
Miao
M
Song
College of Veterinary Medicine, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, China.|College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, China.
Hui
H
Huo
College of Veterinary Medicine, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, China.
Zheng
Z
Cao
College of Veterinary Medicine, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, China.
Yanfei
Y
Han
College of Veterinary Medicine, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, China. gaoli@neau.edu.cn.
Li
L
Gao
D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000494:pharmacology*; D000818:Animals; D002113:Calcification, Physiologic / Q000187:drug effects*; D002478:Cells, Cultured; D002712:Chlorides / Q000494:pharmacology*; D005260:Female; D008297:Male; D010006:Osteoblasts / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D017607:Aluminum Compounds; D002712:Chlorides; D000077410:Aluminum Chloride
Aluminum trichloride; Mineralization; Osteoblasts; Rat