Body Fluid Identification Using mRNA Profiling.
RNA analysis is a valuable tool for the identification of the forensically relevant body fluids, saliva, blood, menstrual blood, cervicovaginal fluid, and semen. Multiple human mRNA and bacterial RNA markers have been identified for each of these body fluids. RNA and DNA can be coextracted from the same portion of a sample and RNA markers for different body fluids can be multiplexed in a single PCR, thereby maximizing the number of analyses that can be performed with limited sample material.
Cellmark, Blacklands Way, Abingdon, Oxfordshire, OX14 1DY, UK. aroeder@cellmark.co.uk.
Amy D
AD
Roeder
Institute of Legal Medicine, University of Zurich, Zürich, Switzerland.
Cordula
C
Haas
D001826:Body Fluids; D016172:DNA Fingerprinting / Q000379:methods; D053803:Forensic Genetics / Q000379:methods*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics*; D017423:Sequence Analysis, RNA / Q000379:methods*
D012333:RNA, Messenger
Body fluid identification; Forensic; mRNA profiling