High-extinction virtually imaged phased array-based Brillouin spectroscopy of turbid biological media.
Brillouin microscopy has recently emerged as a powerful technique to characterize the mechanical properties of biological tissue, cell, and biomaterials. However, the potential of Brillouin microscopy is currently limited to transparent samples, because Brillouin spectrometers do not have sufficient spectral extinction to reject the predominant non-Brillouin scattered light of turbid media. To overcome this issue, we combined a multi-pass Fabry-Perot interferometer with a two-stage virtually imaged phased array spectrometer. The Fabry-Perot etalon acts as an ultra-narrow band-pass filter for Brillouin light with high spectral extinction and low loss. We report background-free Brillouin spectra from Intralipid solutions and up to 100μm deep within chicken muscle tissue.
Fishell Department of Bioengineering, University of Maryland , College Park, College Park, Maryland 20742, USA.
Antonio
0000-0002-5159-9848
A
Fiore
Fishell Department of Bioengineering, University of Maryland , College Park, College Park, Maryland 20742, USA.
Jitao
J
Zhang
Harvard Medical School and Wellman Center for Photomedicine , Massachusetts General Hospital, 50 Blossom St., Boston, Massachusetts 02114, USA.
Peng
0000-0003-2394-0612
P
Shao
Harvard Medical School and Wellman Center for Photomedicine , Massachusetts General Hospital, 50 Blossom St., Boston, Massachusetts 02114, USA.
Seok Hyun
SH
Yun
Fishell Department of Bioengineering, University of Maryland , College Park, College Park, Maryland 20742, USA.
Giuliano
G
Scarcelli