High Spatial Resolution Imaging of Endogenous Hydrogen Peroxide in Living Cells by Solid-State Fluorescence.
Herein, we describe selective imaging of hydrogen peroxide using a precipitating dye conjugated to a boronic acid-based immolative linker. We achieved visualization of endogenous hydrogen peroxide in phagosomes by solid-state two-photon fluorescence imaging in living cells with exceptionally high spatial resolution.
Department of Organic Chemistry, NCCR Chemical Biology University of Geneva, 30 quai Ernest Ansermet, 1211, Geneva, Switzerland.
Eric
E
Lindberg
Department of Organic Chemistry, NCCR Chemical Biology University of Geneva, 30 quai Ernest Ansermet, 1211, Geneva, Switzerland. nicolas.winssinger@unige.ch.
Nicolas
N
Winssinger
D000818:Animals; D001897:Boronic Acids / Q000737:chemistry; D002460:Cell Line; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000032:analysis* / Q000138:chemical synthesis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis*; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton; D015394:Molecular Structure
D001897:Boronic Acids; D005456:Fluorescent Dyes; D006861:Hydrogen Peroxide
fluorescent probes; hydrogen peroxide; imaging; precipitating dye; quinazolinone