Behavioral profile assessment in offspring of Swiss mice treated during pregnancy and lactation with caffeine.
The association between caffeine consumption and various psychiatric manifestations has long been observed. The objective was to assess the behavioral profile in offspring of Swiss mice treated during pregnancy and lactation with caffeine. For this purpose, two groups (n = 6 each and BW ~ 35 g) of female mice were treated during pregnancy and lactation by: tap water and caffeine solution at a concentration of 0.3 mg/mL through oral route. The offspring obtained, by completing 70 days of life, was underwent a behavioral battery test. Statistical analysis was performed by student t test and the different significance adopted was p < 0.05. According to our results, it was not found any significant differences in tail suspension and forced swimming tests. In anxiety related responses however, the mice of caffeine group had greater number of fecal pellets (178 %, p = 0.001) in the open field test, higher number of attempts (51 %, p = 0.03) in light-dark box and decreased percentage of entries in open arms (41 %, p = 0.01) in elevated plus maze test. Moreover, in the marble burying test, there was a significant decrease in the number of buried marbles compared with controls (110 %, p = 0,002). In the meantime, in the von Frey test, it was observed an exacerbation of mechanical allodynia both in basal conditions and after the carrageenan administration (p < 0.001). Furthermore, caffeine treatment during pregnancy and lactation causes long-term behavioral changes in the mice offspring that manifest later in life.
Multicenter Graduate Program in Physiological Sciences, Brazilian Physiological Society, Institute of Biological and Health Sciences, Department of Physiological Sciences, Federal Rural University of Rio de Janeiro, BR 465, Km 7, PQ Room 30, Seropedica, 23897-000, Rio de Janeiro, Brazil.
Roberto
R
Laureano-Melo
Multicenter Graduate Program in Physiological Sciences, Brazilian Physiological Society, Institute of Biological and Health Sciences, Department of Physiological Sciences, Federal Rural University of Rio de Janeiro, BR 465, Km 7, PQ Room 30, Seropedica, 23897-000, Rio de Janeiro, Brazil.|Department of Physical Education, Institute of Education, Federal Rural University of Rio de Janeiro, BR 465, Km 7, 23897-000, Seropédica, Rio de Janeiro, Brazil.
Anderson Luiz Bezerra
AL
da Silveira
Graduate Program in Physiological Sciences, Institute of Biological and Health Sciences, Department of Physiological Sciences, Federal Rural University of Rio de Janeiro, BR 465, Km 7, PQ Room 01, Seropedica, 23897-000, Rio de Janeiro, Brazil.
Fernando
F
de Azevedo Cruz Seara
Multicenter Graduate Program in Physiological Sciences, Brazilian Physiological Society, Institute of Biological and Health Sciences, Department of Physiological Sciences, Federal Rural University of Rio de Janeiro, BR 465, Km 7, PQ Room 30, Seropedica, 23897-000, Rio de Janeiro, Brazil.
Rodrigo Rodrigues
RR
da Conceição
Multicenter Graduate Program in Physiological Sciences, Brazilian Physiological Society, Institute of Biological and Health Sciences, Department of Physiological Sciences, Federal Rural University of Rio de Janeiro, BR 465, Km 7, PQ Room 30, Seropedica, 23897-000, Rio de Janeiro, Brazil.
Cláudio
C
da Silva-Almeida
Multicenter Graduate Program in Physiological Sciences, Brazilian Physiological Society, Institute of Biological and Health Sciences, Department of Physiological Sciences, Federal Rural University of Rio de Janeiro, BR 465, Km 7, PQ Room 30, Seropedica, 23897-000, Rio de Janeiro, Brazil.|Graduate Program in Physiological Sciences, Institute of Biological and Health Sciences, Department of Physiological Sciences, Federal Rural University of Rio de Janeiro, BR 465, Km 7, PQ Room 01, Seropedica, 23897-000, Rio de Janeiro, Brazil.
Bruno Guimarães
BG
Marinho
Multicenter Graduate Program in Physiological Sciences, Brazilian Physiological Society, Institute of Biological and Health Sciences, Department of Physiological Sciences, Federal Rural University of Rio de Janeiro, BR 465, Km 7, PQ Room 30, Seropedica, 23897-000, Rio de Janeiro, Brazil.|Graduate Program in Physiological Sciences, Institute of Biological and Health Sciences, Department of Physiological Sciences, Federal Rural University of Rio de Janeiro, BR 465, Km 7, PQ Room 01, Seropedica, 23897-000, Rio de Janeiro, Brazil.
Fábio Fagundes
FF
da Rocha
Multicenter Graduate Program in Physiological Sciences, Brazilian Physiological Society, Institute of Biological and Health Sciences, Department of Physiological Sciences, Federal Rural University of Rio de Janeiro, BR 465, Km 7, PQ Room 30, Seropedica, 23897-000, Rio de Janeiro, Brazil.|Graduate Program in Physiological Sciences, Institute of Biological and Health Sciences, Department of Physiological Sciences, Federal Rural University of Rio de Janeiro, BR 465, Km 7, PQ Room 01, Seropedica, 23897-000, Rio de Janeiro, Brazil.
Luís Carlos
LC
Reis
Multicenter Graduate Program in Physiological Sciences, Brazilian Physiological Society, Institute of Biological and Health Sciences, Department of Physiological Sciences, Federal Rural University of Rio de Janeiro, BR 465, Km 7, PQ Room 30, Seropedica, 23897-000, Rio de Janeiro, Brazil. wscortes64@gmail.com.|Graduate Program in Physiological Sciences, Institute of Biological and Health Sciences, Department of Physiological Sciences, Federal Rural University of Rio de Janeiro, BR 465, Km 7, PQ Room 01, Seropedica, 23897-000, Rio de Janeiro, Brazil. wscortes64@gmail.com.
Wellington da Silva
Wda S
Côrtes
D000818:Animals; D001007:Anxiety / Q000139:chemically induced / Q000523:psychology; D002110:Caffeine / Q000494:pharmacology* / Q000633:toxicity; D000697:Central Nervous System Stimulants / Q000494:pharmacology* / Q000633:toxicity; D005260:Female; D006930:Hyperalgesia / Q000139:chemically induced / Q000523:psychology; D007774:Lactation / Q000187:drug effects* / Q000502:physiology; D008297:Male; D018782:Maze Learning / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D009043:Motor Activity / Q000187:drug effects* / Q000502:physiology; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000139:chemically induced / Q000523:psychology
D000697:Central Nervous System Stimulants; D002110:Caffeine
Allodynia; Anxiety; Behavior; Caffeine; Mice; Offspring