The B-cell response to foot-and-mouth-disease virus in cattle following vaccination and live-virus challenge.
Antibodies play a pivotal role against viral infection, and maintenance of protection is dependent on plasma and memory B-cells. Understanding antigen-specific B-cell responses in cattle is essential to inform future vaccine design. We have previously defined T-cell-dependent and -independent B-cell responses in cattle, as a prelude to investigating foot-and-mouth-disease-virus (FMDV)-specific B-cell responses. In this study, we have used an FMDV O-serotype vaccination (O1-Manisa or O SKR) and live-virus challenge (FMDV O SKR) to investigate the homologous and heterologous B-cell response in cattle following both vaccination and live-virus challenge. The FMDV O-serotype vaccines were able to induce a cross-reactive plasma-cell response, specific for both O1-Manisa and O SKR, post-vaccination. Post-FMDV O SKR live-virus challenge, the heterologous O1-Manisa vaccination provided cross-protection against O SKR challenge and cross-reactive O SKR-specific plasma cells were induced. However, vaccination and live-virus challenge were not able to induce a detectable FMDV O-serotype-specific memory B-cell response in any of the cattle. The aim of new FMDV vaccines should be to induce memory responses and increased duration of immunity in cattle.
The Pirbright Institute, Ash Road, Woking, UK.
Clare F J
CFJ
Grant
The Pirbright Institute, Ash Road, Woking, UK.
B Veronica
BV
Carr
CSIRO, 5 Portarlington Road, Geelong, VIC 3220, Australia.
Nagendrakumar B
NB
Singanallur
CSIRO, 5 Portarlington Road, Geelong, VIC 3220, Australia.
Jacqueline
J
Morris
The Pirbright Institute, Ash Road, Woking, UK.
Simon
S
Gubbins
Merial Animal Health Ltd, 254 Rue Marcel Mérieux, 69007 Lyon, France.
Pascal
P
Hudelet
Merial Animal Heath Ltd, Ash Road, Woking, UK.
Martin
M
Ilott
Merial Animal Health Ltd, 254 Rue Marcel Mérieux, 69007 Lyon, France.
Catherine
C
Charreyre
CSIRO, 5 Portarlington Road, Geelong, VIC 3220, Australia.
Wilna
W
Vosloo
The Pirbright Institute, Ash Road, Woking, UK.
Bryan
B
Charleston
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001402:B-Lymphocytes / Q000276:immunology*; D002417:Cattle; D056738:Cross Protection; D003429:Cross Reactions; D005536:Foot-and-Mouth Disease / Q000276:immunology*; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D007156:Immunologic Memory; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
D000914:Antibodies, Viral; D014765:Viral Vaccines