TSG-6 treatment promoted apoptosis in human fibroblasts of pathological scar.
Pathological scars are characterized by excessive fibrosis and extracellular matrix (ECM) deposition and can be functionally and cosmetically problematic; however, there are few satisfactory treatments for controlling pathological scars. The proliferation of fibroblast during wound healing play an important role in pathologic scar formation. Therefore, the promotion of fibroblast apoptosis may be a new point to inhibit scar formation. In this study, pathological scar fibroblasts were treated with TSG-6 in monolayer culture and subjected to examination for cell apoptosis and associated proteins involved in cell apoptosis. We found that TSG-6 significantly promoted cell apoptosis of pathological scar fibroblasts. Meanwhile, expression level of cell apoptosis related cytokine protein Bax increased and cell proliferation related signal transduction pathways are blocked since the expression level of Bcl-2 was decreased.
First Affiliated Hospital of Anhui Medical University Department of Plastic Surgery Hefei China.
H
H
Wang
First Affiliated Hospital of Anhui Medical University Department of Plastic Surgery Hefei China.
Z
Z
Chen
First Affiliated Hospital of Anhui Medical University Department of Plastic Surgery Hefei China xiaojingli6@yahoo.com.
X
X
Li
The Second Affiliated Hospital of Xi'an Medical College Intensive Care Unit Xi'an China.
Y
Y
Tang
First Affiliated Hospital of Anhui Medical University Department of Plastic Surgery Hefei China.
X
X
Li
First Affiliated Hospital of Anhui Medical University Department of Plastic Surgery Hefei China.
S
S
Zhang
College of Nursing, Anhui University of Chinese Medicine Aesthetic Medicine Research Office Hefei China.
L
L
Ma
Anhui Medical University Department of Anatomy Hefei China.
X
X
Huang
D017209:Apoptosis / Q000187:drug effects*; D015815:Cell Adhesion Molecules / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002921:Cicatrix / Q000473:pathology*; D016207:Cytokines / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D006801:Humans; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D055815:Young Adult
D015815:Cell Adhesion Molecules; D016207:Cytokines; D018809:Proliferating Cell Nuclear Antigen