GS/DBM/PLA porous composite biomaterial for the treatment of infective femoral condyle defect in rats.
A bone defect resulting from open bone trauma may easily become infected; however, the administration of efficacious systemic antibiotics cannot be performed at safe levels. Previous studies have investigated anti-infective biomaterials that incorporate into bone and facilitate the direct application of high-concentration local antibiotics. In the present study, the effect of a novel porous composite with gentamicin sulfate (GS) in treating infected femoral condyle defects was investigated using a rat model. A novel porous composite biomaterial was prepared based on a supercritical carbon dioxide fluid technique that combined GS, demineralized bone matrix (DBM) and polylactic acid (PLA). A rat femoral condyle fracture model of infection was established. The GS/DBM/PLA composite biomaterial was implanted and its physicochemical characteristics, biocompatibility and ability to facilitate repair of infected bone defect were assessed. The GS/DBM/PLA composite biomaterial maintained the antibiotic activity of GS, with good anti-compression strength, porosity and biocompatibility. The results of the animal experiments indicated that the GS/DBM/PLA composite biomaterial exerted marked anti-infective effects and facilitated bone defect repair, while simultaneously controlling infection. Porous GS/DBM/PLA is therefore a promising composite biomaterial for use in bone tissue engineering.
Department of Human Anatomy, Guangdong Provincial Key Laboratory of Medical Biomechanics, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China; China Institute for Radiation Protection, Taiyuan, Shanxi 030006, P.R. China.
Xiaoming
X
Liu
Department of Human Anatomy, Zunyi Medical College, Zhuhai, Guangdong 519041, P.R. China.
Lin
L
Yang
China Institute for Radiation Protection, Taiyuan, Shanxi 030006, P.R. China.
Jing
J
Li
China Institute for Radiation Protection, Taiyuan, Shanxi 030006, P.R. China.
Yuming
Y
Zhang
China Institute for Radiation Protection, Taiyuan, Shanxi 030006, P.R. China.
Weijun
W
Xu
Department of Medical Imaging, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China.
Yan
Y
Ren
Department of Traditional Chinese Medicine, Shanxi University, Taiyuan, Shanxi 030001, P.R. China.
Biwang
B
Liu
China Institute for Radiation Protection, Taiyuan, Shanxi 030006, P.R. China.
Biao
B
Yang
Department of Human Anatomy, Guangdong Provincial Key Laboratory of Medical Biomechanics, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China; China Institute for Radiation Protection, Taiyuan, Shanxi 030006, P.R. China.
Baoxing
B
Li
bone defect; bone tissue engineering; composite biomaterial; infection