Immunization with inactivated Middle East Respiratory Syndrome coronavirus vaccine leads to lung immunopathology on challenge with live virus.
To determine if a hypersensitive-type lung pathology might occur when mice were given an inactivated MERS-CoV vaccine and challenged with infectious virus as was seen with SARS-CoV vaccines, we prepared and vaccinated mice with an inactivated MERS-CoV vaccine. Neutralizing antibody was induced by vaccine with and without adjuvant and lung virus was reduced in vaccinated mice after challenge. Lung mononuclear infiltrates occurred in all groups after virus challenge but with increased infiltrates that contained eosinophils and increases in the eosinophil promoting IL-5 and IL-13 cytokines only in the vaccine groups. Inactivated MERS-CoV vaccine appears to carry a hypersensitive-type lung pathology risk from MERS-CoV infection that is similar to that found with inactivated SARS-CoV vaccines from SARS-CoV infection.
a Departments of Microbiology and Immunology , University of Texas Medical Branch , Galveston , TX , USA.
Anurodh Shankar
AS
Agrawal
a Departments of Microbiology and Immunology , University of Texas Medical Branch , Galveston , TX , USA.
Xinrong
X
Tao
a Departments of Microbiology and Immunology , University of Texas Medical Branch , Galveston , TX , USA.|e Department of Medical Laboratories Technology , College of Applied Medical Sciences, Jazan University , Jazan , Saudi Arabia.
Abdullah
A
Algaissi
a Departments of Microbiology and Immunology , University of Texas Medical Branch , Galveston , TX , USA.
Tania
T
Garron
a Departments of Microbiology and Immunology , University of Texas Medical Branch , Galveston , TX , USA.
Krishna
K
Narayanan
b Pathology, University of Texas Medical Branch , Galveston , TX , USA.
Bi-Hung
BH
Peng
c Internal Medicine, Division of Infectious Disease, University of Texas Medical Branch , Galveston , TX , USA.
Robert B
RB
Couch
a Departments of Microbiology and Immunology , University of Texas Medical Branch , Galveston , TX , USA.|d Center for Biodefense and Emerging Infectious Disease, University of Texas Medical Branch , Galveston , TX , USA.
Chien-Te K
CT
Tseng
D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000914:Antibodies, Viral / Q000097:blood; D018352:Coronavirus Infections / Q000473:pathology / Q000517:prevention & control*; D006967:Hypersensitivity / Q000473:pathology; D008168:Lung / Q000473:pathology*; D008822:Mice, Transgenic; D065207:Middle East Respiratory Syndrome Coronavirus / Q000276:immunology*; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000009:adverse effects / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D015164:Vaccines, Inactivated; D014765:Viral Vaccines
coronavirus; Eosinophils; immunopathology; Middle East Respiratory Syndrome; vaccination