The cat lipocalin Fel d 7 and its cross-reactivity with the dog lipocalin Can f 1.
We investigated the prevalence of sensitization to the cat lipocalin Fel d 7 among 140 cat-sensitized Swedish patients and elucidated its allergenic activity and cross-reactivity with the dog lipocalin Can f 1. Sixty-five of 140 patients had IgE to rFel d 7 whereof 60 also had IgE to rCan f 1. A moderate correlation between IgE levels to rFel d 7 and rCan f 1 was found. rFel d 7 activated basophils in vitro and inhibited IgE binding to rCan f 1 in 4 of 13 patients, whereas rCan f 1 inhibited IgE binding to rFel d 7 in 7 of 13 patients. Fel d 7 and Can f 1 showed high similarities in protein structure and epitopes in common were found using cross-reactive antisera. Fel d 7 is a common allergen in a Swedish cat-sensitized population that cross-reacts with Can f 1, and may contribute to symptoms in cat- but also in dog-allergic patients.
Department of Medicine Solna, Immunology and Allergy Unit, Karolinska Institutet, and Karolinska University Hospital, Stockholm, Sweden.
D
D
Apostolovic
Department of Medicine Solna, Immunology and Allergy Unit, Karolinska Institutet, and Karolinska University Hospital, Stockholm, Sweden.
S
S
Sánchez-Vidaurre
Department of Medicine Solna, Immunology and Allergy Unit, Karolinska Institutet, and Karolinska University Hospital, Stockholm, Sweden.
K
K
Waden
Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
M
M
Curin
Department of Medicine Solna, Immunology and Allergy Unit, Karolinska Institutet, and Karolinska University Hospital, Stockholm, Sweden.
J
J
Grundström
Department of Clinical Neuroscience, Therapeutic Immune Design Unit, Center for Molecular Medicine (CMM), Karolinska Institutet, Stockholm, Sweden.
G
G
Gafvelin
Center of Excellence for Molecular Food Sciences, Faculty of Chemistry, University of Belgrade, Belgrade, Serbia.
T
T
Cirkovic Velickovic
Department of Clinical Neuroscience, Therapeutic Immune Design Unit, Center for Molecular Medicine (CMM), Karolinska Institutet, Stockholm, Sweden.
H
H
Grönlund
Center for Child Health Research, Telethon Institute of Child Health Research, University of Western Australia, Perth, WA, Australia.
W R
WR
Thomas
Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
R
R
Valenta
Department of Medicine Solna, Immunology and Allergy Unit, Karolinska Institutet, and Karolinska University Hospital, Stockholm, Sweden.|Center for Inflammatory Diseases, Karolinska Institutet, Stockholm, Sweden.
C
C
Hamsten
Department of Medicine Solna, Immunology and Allergy Unit, Karolinska Institutet, and Karolinska University Hospital, Stockholm, Sweden. marianne.van.hage@ki.se.
M
M
van Hage
D000485:Allergens / Q000737:chemistry / Q000276:immunology*; D000818:Animals; D001491:Basophils / Q000276:immunology; D002415:Cats; D003429:Cross Reactions / Q000276:immunology*; D004285:Dogs; D000939:Epitopes / Q000276:immunology; D006967:Hypersensitivity / Q000097:blood / Q000276:immunology; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D054834:Lipocalins / Q000737:chemistry / Q000276:immunology*; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D011487:Protein Conformation; D013548:Sweden
D000485:Allergens; D000939:Epitopes; D054834:Lipocalins; D010455:Peptides; C091462:allergen Can f I; D007073:Immunoglobulin E
Can f 1; Fel d 7; cat allergy; cross-reactivity; dog allergy