The effect of R-(-)-deprenyl administration on antioxidant enzymes in rat testis.
The aim of the study was to investigate the effect of R-(-)-deprenyl administration on the activity and localization of superoxide dismutases (SODs) and catalase (CAT) in rat testis. After 30 days of intraperitoneal administration of either saline (control) or R-(-)-deprenyl dissolved in saline at concentrations of 0.0025mg/kg (low dose of deprenyl, LDD) or 0.25mg/kg (high dose of deprenyl, HDD), males were killed by thiopental, and their testes were collected. We found that deprenyl administration significantly increased the activity of antioxidant enzymes, and this effect varied by dosage. LDD caused significant elevation of all monitored enzymes, but HDD did not increase the activity of SOD2. Employing immunohistochemistry, we detected enzymes predominantly in Leydig cells (SOD1, SOD2, CAT), in late spermatids and residual bodies (SOD1, SOD2), and in primary spermatocytes (SOD2). Histopathological examination did not reveal testicular damage in experimental groups compared to control. Deprenyl proved to be a potent stimulator of antioxidant enzymes in rat testes; therefore, it could be used in the therapy of male infertility. On the other hand, it is crucial to choose a proper dose, since lower dose was more competent compared to a dosage that was one hundred times higher.
Department of Anatomy, Medical Faculty, Šafárik University, Šrobárova 2, 040 01 Košice, Slovakia.
Františka
F
Horváthová
Institute of Neurobiology, Slovak Academy of Sciences, Šoltésovej 4, 040 01 Košice, Slovakia.
Viera
V
Danielisová
Department of Histology and Embryology, Medical Faculty, Šafárik University, Šrobárova 2, 041 80 Košice, Slovakia.
Iveta
I
Domoráková
Institute of Biology and Ecology, Science Faculty, Šafárik University, Moyzesova 11, 040 01 Košice, Slovakia.
Peter
P
Solár
Department of Anatomy, Medical Faculty, Šafárik University, Šrobárova 2, 040 01 Košice, Slovakia.
Silvia
S
Rybárová
Department of Anatomy, Medical Faculty, Šafárik University, Šrobárova 2, 040 01 Košice, Slovakia.
Ingrid
I
Hodorová
Department of Anatomy, Medical Faculty, Šafárik University, Šrobárova 2, 040 01 Košice, Slovakia. Electronic address: jozef.mihalik@upjs.sk.
Jozef
J
Mihalik
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012642:Selegiline / Q000008:administration & dosage* / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism*; D013737:Testis / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*
D000975:Antioxidants; D012642:Selegiline; D002374:Catalase; D013482:Superoxide Dismutase
CAT; Immunohistochemistry; R-(-)-Deprenyl hydrochloride (Selegiline hydrochloride) (PubChem CID: 26758); SOD; Selegiline