Microfluidic integrated acoustic waving for manipulation of cells and molecules.
Acoustophoresis with its simple and low-cost fabrication, rapid and localized fluid actuation, compatibility with microfluidic components, and biocompatibility for cellular studies, has been extensively integrated into microfluidics to provide on-chip microdevices for a variety of applications in biology, bioengineering and chemistry. Among different applications, noninvasive manipulation of cells and biomolecules are significantly important, which are addressed by acoustic-based microfluidics. Here in this paper, we briefly explain the principles and different configurations of acoustic wave and acoustic streaming for the manipulation of cells and molecules and overview its applications for single cell isolation, cell focusing and sorting, cell washing and patterning, cell-cell fusion and communication, and tissue engineering. We further discuss the application of acoustic-based microfluidic systems for the mixing and transport of liquids, manipulation of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) molecules, followed by explanation on the present challenges of acoustic-based microfluidics for the handling of cells and molecules, and highlighting the future directions.
Department of Mechanical Engineering, Isfahan University of Technology, Isfahan, Iran.
Alireza
A
Barani
Department of Mechanical Engineering, Najafabad Branch, Islamic Azad University, Najafabad, Iran.
Hossein
H
Paktinat
BioMEMS and Bioinspired Microfluidic Laboratory, Department of Mechanical and Manufacturing Engineering, University of Calgary, Calgary, Canada; Center for BioEngineering Research and Education, University of Calgary, Calgary, Canada.
Mohsen
M
Janmaleki
Department of Mechanical Engineering, Isfahan University of Technology, Isfahan, Iran.
Aminollah
A
Mohammadi
Department of Mechanical Engineering, Isfahan University of Technology, Isfahan, Iran.
Peiman
P
Mosaddegh
Department of Mechanical Engineering, Isfahan University of Technology, Isfahan, Iran.
Alireza
A
Fadaei-Tehrani
BioMEMS and Bioinspired Microfluidic Laboratory, Department of Mechanical and Manufacturing Engineering, University of Calgary, Calgary, Canada; Center for BioEngineering Research and Education, University of Calgary, Calgary, Canada. Electronic address: amir.sanatinezhad@ucalgary.ca.
Amir
A
Sanati-Nezhad
D000162:Acoustics / Q000295:instrumentation*; D000818:Animals; D002450:Cell Communication; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D008846:Micromanipulation / Q000295:instrumentation / Q000379:methods; D013016:Sound; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods
Acoustic wave; Acoustofluidcs; Manipulation of single cells and molecules; Microfluidics