Survival and Metabolic Activity of Listeria monocytogenes on Ready-to-Eat Roast Beef Stored at 4 °C.
Three brands of commercial roast beef were purchased and artificially inoculated with a 5-strain Listeria monocytogenes cocktail at 2 inoculation levels (approximately 3 and 6 Log CFU/g). Although all 3 brands contained sodium diacetate and sodium lactate, inoculated Listeria cocktail survived for 16 d in all 3 brands; significant increases in L. monocytogenes numbers were seen on inoculated Brand B roast beef on days 12 and 16. Numbers of L. monocytogenes increased to 4.14 Log CFU/g for the 3 Log CFU/g inoculation level and increased to 7.99 Log CFU/g for the 6 Log CFU/g inoculation level by day 16, with the pH values being 5.4 and 5.8 respectively. To measure the cell viability in potential biofilms formed, an Alamar blue assay was conducted. Brand B meat homogenate had the highest metabolic activities (P < 0.05). By comparing its metabolic activities to Brands A and C and the inoculated autoclaved meat homogenates, results indicated that the microflora present in Brand B may be the reason for high metabolic activities. Based on the denaturing gradient gel electrophoresis and the Shannon-Wiener diversity index analysis, the "Brand" factor significantly impacted the diversity index (P = 0.012) and Brand B had the highest microflora diversity (Shannon index 1.636 ± 0.011). Based on this study, results showed that antimicrobials cannot completely inhibit the growth of L. monocytogenes in ready-to-eat roast beef. Native microflora (both diversity and abundance), together with product formula, pH, antimicrobial concentrations, and storage conditions may all impact the survival and growth of L. monocytogenes.
Dept. of Animal Sciences, Auburn Univ, Auburn, AL, 36849.
Johnathan W
JW
Broady
Dept. of Animal Sciences, Auburn Univ, Auburn, AL, 36849.
Dong
D
Han
Dept. of Animal Sciences, Auburn Univ, Auburn, AL, 36849.
Jing
J
Yuan
Dept. of Animal Sciences, Auburn Univ, Auburn, AL, 36849.
Chao
C
Liao
Dept. of Animal Sciences, Auburn Univ, Auburn, AL, 36849.
Christy L
CL
Bratcher
College of Sciences and Mathematics, Auburn Univ, Auburn, AL, 36849.
Mark R
MR
Lilies
College of Sciences and Mathematics, Auburn Univ, Auburn, AL, 36849.
Elizabeth H
EH
Schwartz
Dept. of Animal Sciences, Auburn Univ, Auburn, AL, 36849.
Luxin
L
Wang
D000085:Acetates; D000818:Animals; D000890:Anti-Infective Agents; D044822:Biodiversity; D018441:Biofilms; D002417:Cattle; D015169:Colony Count, Microbial; D005516:Food Microbiology; D005519:Food Preservation / Q000379:methods*; D005520:Food Preservatives; D061353:Food Storage / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008089:Listeria monocytogenes / Q000254:growth & development* / Q000378:metabolism*; D008461:Meat Products / Q000382:microbiology; D000069466:Red Meat / Q000382:microbiology*; D019354:Sodium Lactate; D013696:Temperature
D000085:Acetates; D000890:Anti-Infective Agents; D005520:Food Preservatives; C548838:sodium diacetate; D019354:Sodium Lactate
Listeria monocytogenes; metabolic activity; roast beef; survival