Melinjo (Gnetum gnemon) extract intake during lactation stimulates hepatic AMP-activated protein kinase in offspring of excessive fructose-fed pregnant rats.
Excessive maternal fructose intake during pregnancy and in early postnatal life has metabolic consequences for the offspring. We investigated the effects of melinjo (Gnetum gnemon) extract (MeE) intake during lactation on the expression and phosphorylation of adenosine monophosphate-activated protein kinase (AMPK) in the liver of offspring from excessive fructose-fed pregnant dams. Pregnant Wistar rats received a normal diet and 100g/L fructose solution during gestation ad libitum. At delivery, dams were divided into two groups: a control diet (FC) or a 0.1% MeE-containing diet (FM) fed during lactation. The dams that were not treated with fructose were fed a control diet (CC). At postnatal week 3, some pups were sacrificed, while the remaining continued to receive a normal diet and were sacrificed at week 17. Blood chemistry and phosphorylation levels of AMPK and acetyl-coenzyme A carboxylase (ACC) were evaluated. Plasma glucose levels in FC female offspring increased compared to that receiving CC at weeks 3 and 17; however, the levels in FM female offspring decreased at week 17. The insulin levels in FM female offspring decreased significantly compared to that in FC female offspring at week 3. Hepatic AMPK phosphorylation was upregulated in FM offspring at week 3 and in female, but not male, offspring at week 17. ACC phosphorylation in FM female offspring was upregulated at week 17. Our results suggest that maternal MeE intake during lactation may modulate the hepatic AMPK pathways in female offspring.
Department of Nutrition, Faculty of Health Sciences, Aomori University of Health and Welfare, Aomori 030-8505, Japan.
Saori
S
Kataoka
School of Nutrition and Dietetics, Faculty of Health and Social Work, Kanagawa University of Human Services, Kanagawa 238-8522, Japan.
Yuuka
Y
Mukai
Department of Nutrition, Faculty of Health Sciences, Aomori University of Health and Welfare, Aomori 030-8505, Japan.
Mihoko
M
Takebayashi
Department of Nutrition, Faculty of Health Sciences, Aomori University of Health and Welfare, Aomori 030-8505, Japan.
Megumi
M
Kudo
Graduate School of Environmental Science, Hokkaido University, Sapporo 060-0810, Japan.
Uson Rachael
UR
Acuram
Graduate School of Environmental Science, Hokkaido University, Sapporo 060-0810, Japan.
Masaaki
M
Kurasaki
Department of Nutrition, Faculty of Health Sciences, Aomori University of Health and Welfare, Aomori 030-8505, Japan. Electronic address: s_sato3@auhw.ac.jp.
Shin
S
Sato
D055372:AMP-Activated Protein Kinases / Q000378:metabolism*; D000105:Acetyl Coenzyme A / Q000378:metabolism; D000818:Animals; D005260:Female; D005632:Fructose / Q000494:pharmacology*; D035684:Gnetum; D007328:Insulin / Q000097:blood; D007774:Lactation / Q000187:drug effects* / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D010766:Phosphorylation / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012737:Sex Factors
D007328:Insulin; D010936:Plant Extracts; D005632:Fructose; D000105:Acetyl Coenzyme A; D055372:AMP-Activated Protein Kinases
AMP-activated protein kinase; Fructose; Liver; Melinjo (Gnetum gnemon); Offspring