Nucleosomes determine their own patch size in base excision repair.
Base excision repair (BER) processes non-helix distorting lesions (e.g., uracils and gaps) and is composed of two subpathways that differ in the number of nucleotides (nts) incorporated during the DNA synthesis step: short patch (SP) repair incorporates 1 nt and long patch (LP) repair incorporates 2-12 nts. This choice for either LP or SP repair has not been analyzed in the context of nucleosomes. Initial studies with uracil located in nucleosome core DNA showed a distinct DNA polymerase extension profile in cell-free extracts that specifically limits extension to 1 nt, suggesting a preference for SP BER. Therefore, we developed an assay to differentiate long and short repair patches in 'designed' nucleosomes containing a single-nucleotide gap at specific locations relative to the dyad center. Using cell-free extracts or purified enzymes, we found that DNA lesions in the nucleosome core are preferentially repaired by DNA polymerase β and there is a significant reduction in BER polymerase extension beyond 1 nt, creating a striking bias for incorporation of short patches into nucleosomal DNA. These results show that nucleosomes control the patch size used by BER.
School of Molecular Biosciences, Washington State University, Pullman, WA, USA.
Rithy
R
Meas
School of Molecular Biosciences, Washington State University, Pullman, WA, USA.
Michael J
MJ
Smerdon
D002474:Cell-Free System; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D019951:DNA Polymerase beta / Q000737:chemistry / Q000378:metabolism; D004260:DNA Repair; D006801:Humans; D009707:Nucleosomes / Q000235:genetics*; D014498:Uracil / Q000378:metabolism
D009707:Nucleosomes; D014498:Uracil; D004247:DNA; D019951:DNA Polymerase beta