Synthesis of molecular imprinted polymers for selective extraction of domperidone from human serum using high performance liquid chromatography with fluorescence detection.
In this study a novel method is described for selective quantization of domperidone in biological matrices applying molecular imprinted polymers (MIPs) as a sample clean up procedure using high performance liquid chromatography coupled with a fluorescence detector. MIPs were synthesized with chloroform as the porogen, ethylene glycol dimethacrylate as the crosslinker, methacrylic acid as the monomer, and domperidone as the template molecule. The new imprinted polymer was used as a molecular sorbent for separation of domperidone from serum. Molecular recognition properties, binding capacity and selectivity of MIPs were determined. The results demonstrated exceptional affinity for domperidone in biological fluids. The domperidone analytical method using MIPs was verified according to validation parameters, such as selectivity, linearity (5-80ng/mL, r(2)=0.9977), precision and accuracy (10-40ng/mL, intra-day=1.7-5.1%, inter-day=4.5-5.9%, and accuracy 89.07-98.9%).The limit of detection (LOD) and quantization (LOQ) of domperidone was 0.0279 and 0.092ng/mL, respectively. The simplicity and suitable validation parameters makes this a highly valuable selective bioequivalence method for domperidone analysis in human serum.
Department of Medicinal Chemistry; School of Pharmacy, Shiraz University of Medical Sciences (SUMS), Shiraz, 7146864685, Iran. Electronic address: simin.salehi@gmail.com.
Simin
S
Salehi
Department of Medicinal Chemistry; School of Pharmacy, Shiraz University of Medical Sciences (SUMS), Shiraz, 7146864685, Iran; Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences (SUMS), Shiraz, 7146864685, Iran. Electronic address: rasoulamini@sums.ac.ir.
Sara
S
Rasoul-Amini
Food and Drug Laboratory Research Center, Food and Drug Organization, MOH & ME, Tehran, 1113615911, Iran. Electronic address: nadib50@yahoo.com.
Noushin
N
Adib
Food and Drug Laboratory Research Center, Food and Drug Organization, MOH & ME, Tehran, 1113615911, Iran. Electronic address: shekarchim@yahoo.com.
Maryam
M
Shekarchi
D000327:Adsorption; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004294:Domperidone / Q000097:blood* / Q000302:isolation & purification; D018492:Dopamine Antagonists / Q000097:blood* / Q000302:isolation & purification; D005453:Fluorescence; D006801:Humans; D057230:Limit of Detection; D008689:Methacrylates / Q000737:chemistry*; D054802:Molecular Imprinting / Q000379:methods*; D052616:Solid Phase Extraction / Q000379:methods
D018492:Dopamine Antagonists; D008689:Methacrylates; C008384:methacrylic acid; D004294:Domperidone; C004919:ethylene dimethacrylate
Domperidone; HPLC; Human serum; Molecular imprinted polymer; Selective extraction