Thailandins A and B, New Polyene Macrolactone Compounds Isolated from Actinokineospora bangkokensis Strain 44EHW(T), Possessing Antifungal Activity against Anthracnose Fungi and Pathogenic Yeasts.
Two new polyene macrolactone antibiotics, thailandins A, 1, and B, 2, were isolated from the fermentation broth of rhizosphere soil-associated Actinokineospora bangkokensis strain 44EHW(T). The new compounds from this strain were purified using semipreparative HPLC and Sephadex LH-20 gel filtration while following an antifungal activity guided fractionation. Their structures were elucidated through spectroscopic techniques including UV, HR-ESI-MS, and NMR. These compounds demonstrated broad spectrum antifungal activity against fungi causing anthracnose disease (Colletotrichum gloeosporioides DoA d0762, Colletotrichum gloeosporiodes DoA c1060, and Colletotrichum capsici DoA c1511) as well as pathogenic yeasts (Candida albicans MT 2013/1, Candida parasilopsis DKMU 434, and Cryptococcus neoformans MT 2013/2) with minimum inhibitory concentrations ranging between 16 and 32 μg/mL. This is the first report of polyene antibiotics produced by Actinokineospora species as bioactive compounds against anthracnose fungi and pathogenic yeast strains.
Department of Biotechnology, Faculty of Science, Mahidol University , 272 Rama 6 Road, Bangkok 10400, Thailand.|Mahidol University and Osaka Collaborative Research Center on Bioscience and Biotechnology , Bangkok 10400, Thailand.
Bungonsiri
B
Intra
Institute for Pharmaceutical Biology and Biotechnology, Albert-Ludwigs University of Freiburg , Stefan-Meier-Strasse 19, 79104 Freiburg, Germany.
Anja
A
Greule
Institute for Pharmaceutical Biology and Biotechnology, Albert-Ludwigs University of Freiburg , Stefan-Meier-Strasse 19, 79104 Freiburg, Germany.
Andreas
A
Bechthold
Department of Biotechnology, Faculty of Science, Mahidol University , 272 Rama 6 Road, Bangkok 10400, Thailand.|Mahidol University and Osaka Collaborative Research Center on Bioscience and Biotechnology , Bangkok 10400, Thailand.
Jirayut
J
Euanorasetr
Department of Chemistry-Biology, Organic Chemistry II, University of Siegen , Adolf-Reichwein-Strasse 2, 57068 Siegen, Germany.
Thomas
T
Paululat
Department of Biotechnology, Faculty of Science, Mahidol University , 272 Rama 6 Road, Bangkok 10400, Thailand.|Mahidol University and Osaka Collaborative Research Center on Bioscience and Biotechnology , Bangkok 10400, Thailand.
Watanalai
W
Panbangred
D039903:Actinobacteria / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D000935:Antifungal Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects; D020231:Colletotrichum / Q000187:drug effects; D003455:Cryptococcus neoformans / Q000187:drug effects; D005285:Fermentation; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D010935:Plant Diseases / Q000382:microbiology*; D011090:Polyenes / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D013785:Thailand
D000935:Antifungal Agents; D011090:Polyenes
Actinokineospora; Actinokineospora bangkokensis; anthracnose; polyene; rhizospheric soil