A New Temperature Control Shifting Strategy for Enhanced Triterpene Production by Ganoderma lucidum G0119 Based on Submerged Liquid Fermentation.
Temperature control is a very important factor on triterpene productivity in submerged liquid fermentation. Temperature effects from 23 to 32 °C on triterpene production by Ganoderma lucidum G0119 were investigated in 6-L stirred fermentor. Logistic and Luedeking-Piret equations were used to estimate the mycelial growth and triterpene production kinetics by regression analysis. On that basis, a temperature-shifting fermentation control strategy was established. From 0 to 61 h, culturing was performed at 32 °C to get high specific mycelial growth rate. Between 62 and 127 h, the temperature was decreased stepwise from 31 to 30 °C to maintain high triterpene productivity. After 128 h, temperature was maintained at 29 °C to minimize triterpene production inhibition and sustain high productivity. Elevated triterpene level (0.269 g L-1), yield (0.0101 g g-1), and productivity (0.00207 g (L h)-1) were achieved representing 27.32, 13.94, and 37.11 % higher than submerged liquid fermentation at constant temperature of 29 °C, respectively, feasible for the industrial scale.
Key Laboratory of Edible Fungi Resources and Utilization (South) of Ministry of Agriculture, Shanghai, China.|National Engineering Research Center of Edible Fungi, Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Shanghai, China.|Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, 1000 Jinqi Road, Fengxian District, Shanghai, 201403, China.
Jie
J
Feng
Key Laboratory of Edible Fungi Resources and Utilization (South) of Ministry of Agriculture, Shanghai, China.|National Engineering Research Center of Edible Fungi, Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Shanghai, China.|Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, 1000 Jinqi Road, Fengxian District, Shanghai, 201403, China.
Na
N
Feng
Key Laboratory of Edible Fungi Resources and Utilization (South) of Ministry of Agriculture, Shanghai, China. syja16@saas.sh.cn.|National Engineering Research Center of Edible Fungi, Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Shanghai, China. syja16@saas.sh.cn.|Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, 1000 Jinqi Road, Fengxian District, Shanghai, 201403, China. syja16@saas.sh.cn.
Jing-Song
JS
Zhang
Key Laboratory of Edible Fungi Resources and Utilization (South) of Ministry of Agriculture, Shanghai, China.|National Engineering Research Center of Edible Fungi, Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Shanghai, China.|Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, 1000 Jinqi Road, Fengxian District, Shanghai, 201403, China.
Yan
Y
Yang
Key Laboratory of Edible Fungi Resources and Utilization (South) of Ministry of Agriculture, Shanghai, China.|National Engineering Research Center of Edible Fungi, Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Shanghai, China.|Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, 1000 Jinqi Road, Fengxian District, Shanghai, 201403, China.
Wei
W
Jia
Key Laboratory of Edible Fungi Resources and Utilization (South) of Ministry of Agriculture, Shanghai, China.|National Engineering Research Center of Edible Fungi, Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Shanghai, China.|Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, 1000 Jinqi Road, Fengxian District, Shanghai, 201403, China.
Chi-Chung
CC
Lin
D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D008962:Models, Theoretical; D025282:Mycelium / Q000254:growth & development; D020940:Reishi / Q000378:metabolism*; D015203:Reproducibility of Results; D013696:Temperature; D014315:Triterpenes / Q000378:metabolism*
D014315:Triterpenes; D005947:Glucose
Ganoderma lucidum; Kinetics model; Mycelium; Temperature-shifting strategy; Triterpenes