Curcumin nanoparticles loaded hydrogels protects against aflatoxin B1-induced genotoxicity in rat liver.
The current study aimed to evaluate the protective role of curcumin nanoparticles loaded hydrogels (Cur-NPs-Hgs) against AFB1-induced genotoxicity in rat liver. Animals were divided into 7 treatment groups and treated orally for 3 weeks as follow: the control group, the group treated with Hgs alone (0.5 ml/rat), the groups treated with low or high dose of Cur-NPs-Hgs (100 or 200 mg/kg b.w), the group treated with AFB1 (0.125 mg/kg b.w) and the groups treated with AFB1 plus the low or high dose of Cur-NPs-Hgs. Blood ant liver samples were collected for different biochemical, genetical, histological and histochemical analysis. The results revealed that the prepared Cur-NPs have nearly spherical shape with average size of 140 ± 20 nm and negative zeta potential value of 30.7 ± 2.57 mV. The in vivo results showed that treatment with AFB1 decreased the body weight accompanied biochemical, genotoxicity and histological disturbances. The combined treatment with AFB1 and Cur-Nps-Hgs at the two tested doses succeeded to induce a significant protection against AFB1. It could be concluded that Cur-NPs-Hgs is a promise candidate to protect against AFB1-induce liver damage in the high incidence area. Moreover, Hgs are excellent candidates as drug delivery system.
Food Toxicology and Contaminants Department, National Research Center, Dokki, Cairo, Egypt. Electronic address: mosaad_abdelwahhab@yahoo.com.
Mosaad A
MA
Abdel-Wahhab
Genetic and Cytology Department, National Research Center, Dokki, Cairo, Egypt.
Asmaa S
AS
Salman
Food Toxicology and Contaminants Department, National Research Center, Dokki, Cairo, Egypt.
Mohamed I M
MI
Ibrahim
Food Toxicology and Contaminants Department, National Research Center, Dokki, Cairo, Egypt.
Ahmed A
AA
El-Kady
Cell Biology Department, National Research Center, Dokki, Cairo, Egypt.
Sekena H
SH
Abdel-Aziem
Pathology Department, National Research Center, Dokki, Cairo, Egypt.
Nabila S
NS
Hassan
Preperation and Finishing of Cellulosic Materials Department, National Research Center, Dokki, Cairo, Egypt.
Ahmed I
AI
Waly
D016604:Aflatoxin B1 / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D003474:Curcumin / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D020100:Hydrogels; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008297:Male; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D053758:Nanoparticles; D051381:Rats; D017207:Rats, Sprague-Dawley
D020100:Hydrogels; D009153:Mutagens; D016604:Aflatoxin B1; D003474:Curcumin
Aflatoxin B(1); Gene expression; Genotoxicity; Hydrogels; Liver; curcumin nanoparticles