Asymmetrical proliferative pattern loss linked to cyclin D1 overexpression during malignant transformation of the lip epithelium.
BACKGROUND
There is inadequate knowledge on the involvement of oncogenic mechanisms linked to the cyclin (CCND1) gene in lip squamous cell carcinoma (LSCC).


OBJECTIVE
The aim of this study was to analyse the implication of cyclin D1 in the malignant transformation of lip lesions.


METHODS
We immunohistochemically studied 45 actinic cheilitis cases (15 mild dysplasia, 15 moderate dysplasia, 15 severe dysplasia/carcinoma in situ), 30 LSCC cases with adjacent non-tumour epithelium and 15 normal oral epithelium samples for detection of cyclin D1, β-catenin and Ki-67.


RESULTS
Cyclin D1 and Ki-67 expressions were significantly increased in the basal layer of premalignant epithelia and peripheral layers of tumour nests vs.


METHODS
Premalignant epithelia had lost their asymmetrical proliferative pattern.


CONCLUSIONS
Lip carcinogenesis was associated with loss of the asymmetrical proliferative pattern, a preventive mechanism against lip oncogenesis, and with cyclin D1 overexpression.
Stomatology Department, Area of Pathology, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.|Oral Medicine Department, School of Dentistry, Instituto de Biomedicina, University of Granada, Granada, Spain.
N G
NG
Garcia
Oral Medicine Department, School of Dentistry, Instituto de Biomedicina, University of Granada, Granada, Spain.
M A
MA
González-Moles
Unidad de Gestión Clínica de Anatomía Patológica, Instituto de Biomedicina de Granada Complejo Hospitalario san Cecilio, Granada, Spain.
I
I
Ruiz-Ávila
Oral Medicine Department, School of Dentistry, Instituto de Biomedicina, University of Granada, Granada, Spain.
M
M
Bravo
Oral Medicine Department, School of Dentistry, Instituto de Biomedicina, University of Granada, Granada, Spain.
P
P
Ramos-García
Pathology Department, Botucatu School of Medicine, State University of São Paulo, Botucatu, São Paulo, Brazil.
E M
EM
Minicucci
Pathology Department, Botucatu School of Medicine, State University of São Paulo, Botucatu, São Paulo, Brazil.
M A C
MA
Domingues
Stomatology Department, Area of Pathology, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.
D T
DT
Oliveira
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D049109:Cell Proliferation; D002648:Child; D019938:Cyclin D1 / Q000378:metabolism*; D005260:Female; D006801:Humans; D008046:Lip / Q000378:metabolism / Q000473:pathology*; D008048:Lip Neoplasms / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D055815:Young Adult
D019938:Cyclin D1