Embryonic develop-associated gene 1 is overexpressed and acts as a tumor promoter in thyroid carcinoma.
Embryonic develop-associated gene 1 (EDAG-1), a hematopoietic tissue-specific protein, is usually highly expressed in the placenta, fetal liver, bone marrow and leukemia cells, but the expression status in normal or solid tumor tissues is rarely reported. In this study, we found that EDAG-1 was up-regulated in thyroid carcinoma tissues and cells. Knockdown of EDAG-1 suppressed proliferation and enhanced cisplatin-induced apoptosis of thyroid carcinoma cells. We also demonstrated that knockdown of EDAG-1 inactivated the phosphatidylinositol-3 kinase (PI3K)/Akt signaling pathway in vitro and in vivo. Moreover, knockdown of EDAG-1 suppressed tumorigenesis of thyroid carcinoma in vivo. Taken together, these results suggest that EDAG-1 regulates the proliferation and apoptosis of thyroid carcinoma via the PI3K/Akt signaling pathway.
Department of General Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Shaanxi, PR China.
Jinkai
J
Xu
Department of General Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Shaanxi, PR China.
Zongyu
Z
Li
Department of General Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Shaanxi, PR China.
Qinghua
Q
Su
Department of General Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Shaanxi, PR China.
Jun
J
Zhao
Department of General Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Shaanxi, PR China. Electronic address: jcangma@163.com.
Jiancang
J
Ma
D000818:Animals; D063646:Carcinogenesis / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology / Q000627:therapeutic use; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D008297:Male; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D058539:Phosphatidylinositol 3-Kinase / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000473:pathology*; D015854:Up-Regulation / Q000235:genetics
C435745:HEMGN protein, human; D009687:Nuclear Proteins; D058539:Phosphatidylinositol 3-Kinase; D051057:Proto-Oncogene Proteins c-akt; D002945:Cisplatin
Apoptosis; Embryonic develop-associated gene 1 (EDAG-1); PI3K/Akt pathway; Proliferation; Thyroid carcinoma