Characterization of the interactions between Escherichia coli receptors, LPS and OmpC, and bacteriophage T4 long tail fibers.
Bacteriophages have strict host specificity and the step of adsorption is one of key factors for determining host specificity. Here, we systematically examined the interaction between the Escherichia coli receptors lipopolysaccharide (LPS) and outer membrane protein C (OmpC), and the long tail fibers of bacteriophage T4. Using a variety of LPS mutants, we demonstrated that T4 has no specificity for the sugar sequence of the outer core (one of three LPS regions) in the presence of OmpC but, in the absence of OmpC, can adsorb to a specific LPS which has only one or two glucose residues without a branch. These results strengthen the idea that T4 adsorbs to E. coli via two distinct modes, OmpC-dependent and OmpC-independent, suggested by previous reports (Prehm et al. 1976; Yu and Mizushima 1982). Isolation and characterization of the T4 mutants Nik (No infection to K-12 strain), Nib (No infection to B strain), and Arl (altered recognition of LPS) identified amino acids of the long tail fiber that play important roles in the interaction with OmpC or LPS, suggesting that the top surface of the distal tip head domain of T4 long tail fibers interacts with LPS and its lateral surface interacts with OmpC.
Department of Biological Sciences, Graduate School of Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka, Osaka, 560-0043, Japan.
Ayaka
A
Washizaki
Department of Biological Sciences, Graduate School of Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka, Osaka, 560-0043, Japan.
Tetsuro
T
Yonesaki
Department of Microbiology, School of Medicine, Dokkyo Medical University, 880 Kitakobayashi, Mibu-machi, Shimotsuga-gun, Tochigi, 321-0293, Japan.
Yuichi
Y
Otsuka
D019943:Amino Acid Substitution / Q000235:genetics; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D017122:Bacteriophage T4 / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism / Q000821:virology*; D058507:Host Specificity / Q000502:physiology*; D008070:Lipopolysaccharides / Q000378:metabolism*; D018272:Porins / Q000378:metabolism*; D011991:Receptors, Virus / Q000378:metabolism*; D017376:Viral Tail Proteins / Q000235:genetics / Q000378:metabolism*; D053585:Virus Attachment
D001425:Bacterial Outer Membrane Proteins; D008070:Lipopolysaccharides; C113724:OmpC protein; D018272:Porins; D011991:Receptors, Virus; D017376:Viral Tail Proteins

Escherichia coli
; 
LPS
; Adsorption; bacteriophage T4; ompc; tail fibers