Aqueous Extract of Paris polyphylla (AEPP) Inhibits Ovarian Cancer via Suppression of Peroxisome Proliferator-Activated Receptor-Gamma Coactivator (PGC)-1alpha.
Chemotherapy, a major approach was used in carcinoma treatment, always involves the development of drug resistance as well as side-effects that affect the quality of patients' lives. An association between epithelial-mesenchymal transition (EMT) and chemotherapy resistance was established recently. We demonstrate in this paper that the aqueous extract of Paris polyphylla (AEPP)-a traditional Chinese medicine-can be used in various cancer types for suppression of carcinogenesis. We evaluated the suppressions of EMT and mitochondrial activity by AEPP treatment in a high-glucose (HG) induced-human ovarian carcinoma cell line (OVCAR-3 cells). The mitochondrial morphology was investigated using MitoTracker Deep Red FM staining. Our results indicated that AEPP reduced the viability of OVCAR-3 cells considerably through induction of apoptosis. However, this inhibitory potential of AEPP was attenuated by HG induction in OVCAR-3 cells. The levels of estrogen-related receptor (ERR)-alpha activator and peroxisome proliferator-activated receptor-gamma coactivator (PGC)-1alpha were elevated by HG induction, but were suppressed by AEPP treatment. Down-regulations of cell survival and EMT were oberved in OVCAR-3 cells through suppression of PGC-1alpha by AEPP treatment. These results were confirmed through PGC-1alpha knockdown and overexpression in OVCAR-3 cells. Thus, AEPP can be beneficial for treating ovarian cancer and has potential for development of an integrative cancer therapy against ovarian cancer proliferation, metastasis, and migration.
Department of Obstetrics and Gynecology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11042, Taiwan. cwwang@ms4.hinet.net.|Department of Obstetrics and Gynecology, Center for Reproductive Medicine and Sciences, Taipei Medical University Hospital, Taipei 11042, Taiwan. cwwang@ms4.hinet.net.|Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei 11042, Taiwan. cwwang@ms4.hinet.net.
Chia-Woei
CW
Wang
Division of Hematology and Oncology, Department of Internal Medicine, Taipei Medicine University Hospital, Taipei 11031, Taiwan. cjtai@tmu.edu.tw.|Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan. cjtai@tmu.edu.tw.
Cheng-Jeng
CJ
Tai
Department of Obstetrics and Gynecology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11042, Taiwan. chenyen_318@hotmail.com.
Chen-Yen
CY
Choong
Taiwan Indigena Botanica Co., Ltd., Taipei 11031, Taiwan. doclin10@gmail.com.
Yu-Chun
YC
Lin
Division of Hematology and Oncology, Department of Internal Medicine, Taipei Medicine University Hospital, Taipei 11031, Taiwan. f96b47117@ntu.edu.tw.
Bao-Hong
BH
Lee
Taiwan Indigena Botanica Co., Ltd., Taipei 11031, Taiwan. jasmineycs@yahoo.com.tw.
Yeu-Ching
YC
Shi
Department of Obstetrics and Gynecology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11042, Taiwan. d94b42005@ntu.edu.tw.|Department of Chinese Medicine, Taipei University Hospital, Taipei 11042, Taiwan. d94b42005@ntu.edu.tw.|Traditional Herbal Medicine Research Center, Taipei Medical University Hospital, Taipei 11042, Taiwan. d94b42005@ntu.edu.tw.
Chen-Jei
CJ
Tai
D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008516:Medicine, Chinese Traditional; D000070556:Melanthiaceae / Q000737:chemistry*; D010051:Ovarian Neoplasms / Q000188:drug therapy* / Q000473:pathology; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha / Q000096:biosynthesis* / Q000235:genetics; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry
C486497:PPARGC1A protein, human; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D010936:Plant Extracts
Paris polyphylla; epithelial-mesenchymal transition (EMT); high glucose induction; ovarian cancer; peroxisome proliferator-activated receptor-gamma coactivator (PGC)-1alpha