In Vivo Cell Tracking Using Two-Photon Microscopy.
Recently we have explored and developed approaches imaging using confocal/two-photon microscopy, which enables simultaneous high-resolution assessment of specifically fluorescently marked cells in conjunction with structural components of the tissues visualized via harmonic generated signals. This approach uses commercially available confocal and two-photon laser microscope and automated user-interactive image analysis methods based on commercially available software packages allowing easy implementation in usual microscopy facilities.
Light Microscopy Core Facility, National Heart, Lung, and Blood Institute, National Institute of Health, Building 10, Room 6 N-309, 10 Center Drive, Bethesda, MD, 20892, USA. dmalide@nih.gov.
Daniela
D
Malide
D000818:Animals; D058948:Cell Tracking / Q000295:instrumentation* / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000295:instrumentation; D051379:Mice; D018613:Microscopy, Confocal / Q000295:instrumentation; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation* / Q000379:methods; D012984:Software; D014584:User-Computer Interface
Adipose tissue; Cell tracking; Fluorescent proteins; Lymph node; Multiphoton microscopy; Second harmonic generation; Third harmonic generation