Clostridium difficile Infections in Children: Impact of the Diagnostic Method on Infection Rates.
BACKGROUND Polymerase chain reaction (PCR) assays based on the detection of the toxin B gene are replacing enzyme-linked immunosorbent assay (ELISA)-based toxin production detection or cell cytotoxicity assay in most laboratories. OBJECTIVE To determine the proportion of pediatric patients diagnosed with Clostridium difficile infection by PCR who would have also been diagnosed by ELISA and to compare the clinical characteristics of PCR+/ELISA+ vs PCR+/ELISA- patients. METHODS Using the microbiology laboratory information system, stool samples positive for C. difficile by PCR between October 2010 and July 2014 were identified. Using frozen stool specimens, an ELISA for toxin A and B was performed. A retrospective medical chart review was conducted to obtain demographic and clinical data. Duplicate samples were excluded. RESULTS A total of 136 PCR-positive samples underwent ELISA testing: 54 (40%) were positive for toxin A or B. The mean (SD) age of the entire cohort was 8.5 (6.2) years. There was no difference in age, gender, clinical manifestation, previous medical problems, and management between patients positive or negative by ELISA. However, patients positive by ELISA were more likely to have had a recent exposure to antibiotics (67.9% vs 50%; crude odds ratio, 2.1 [95% CI, 1.03-4.28]). CONCLUSION In our pediatric population, 60% of patients with C. difficile diagnosed by PCR had no toxin detectable by ELISA. ELISA-negative patients were less likely to have received an antibiotic recently compared with ELISA-positive patients. These results highlight the need to standardize laboratory criteria for the diagnosis of C. difficile infections in children. Infect Control Hosp Epidemiol 2016;37:1087-1093.
1Infectious Disease Division,Departments of Pediatrics & Medical Microbiology,Montreal Children's Hospital of the McGill University Health Center,McGill University,Montreal,Canada.
Mohammad
M
AlGhounaim
2Infection Prevention & Control Service,Department of Medicine,Jewish General Hospital,McGill University,Montreal,Canada.
Yves
Y
Longtin
3Research Institute of the McGill University Health Center,Montreal,Canada.
Milagros
M
Gonzales
1Infectious Disease Division,Departments of Pediatrics & Medical Microbiology,Montreal Children's Hospital of the McGill University Health Center,McGill University,Montreal,Canada.
Joanna
J
Merckx
3Research Institute of the McGill University Health Center,Montreal,Canada.
Nicholas
N
Winters
1Infectious Disease Division,Departments of Pediatrics & Medical Microbiology,Montreal Children's Hospital of the McGill University Health Center,McGill University,Montreal,Canada.
Caroline
C
Quach
D000293:Adolescent; D001427:Bacterial Toxins / Q000302:isolation & purification*; D002648:Child; D002675:Child, Preschool; D016360:Clostridioides difficile / Q000302:isolation & purification; D003015:Clostridium Infections / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000382:microbiology; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D016133:Polymerase Chain Reaction; D011792:Quebec; D012189:Retrospective Studies; D012680:Sensitivity and Specificity
D001427:Bacterial Toxins