Characteristics of carboxylesterase genes and their expression-level between acaricide-susceptible and resistant Tetranychus cinnabarinus (Boisduval).
Carboxylesterases (CarEs) play important roles in metabolism and detoxification of dietary and environmental xenobiotics in insects and mites. On the basis of the Tetranychuscinnabarinus transcriptome dataset, 23 CarE genes (6 genes are full sequence and 17 genes are partial sequence) were identified. Synergist bioassay showed that CarEs were involved in acaricide detoxification and resistance in fenpropathrin- (FeR) and cyflumetofen-resistant (CyR) strains. In order to further reveal the relationship between CarE gene's expression and acaricide-resistance in T. cinnabarinus, we profiled their expression in susceptible (SS) and resistant strains (FeR, and CyR). There were 8 and 4 over-expressed carboxylesterase genes in FeR and CyR, respectively, from which the over-expressions were detected at mRNA level, but not DNA level. Pesticide induction experiment elucidated that 4 of 8 and 2 of 4 up-regulated genes were inducible with significance in FeR and CyR strains, respectively, but they could not be induced in SS strain, which indicated that these genes became more enhanced and effective to withstand the pesticides' stress in resistant T. cinnabarinus. Most expression-changed and all inducible genes possess the Abhydrolase_3 motif, which is a catalytic domain for hydrolyzing. As a whole, these findings in current study provide clues for further elucidating the function and regulation mechanism of these carboxylesterase genes in T. cinnabarinus' resistance formation.
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China.
Peng
P
Wei
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China.
Li
L
Shi
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China.
Guangmao
G
Shen
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China.
Zhifeng
Z
Xu
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China.
Jialu
J
Liu
College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, China. Electronic address: ipying@163.com.
Yu
Y
Pan
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China. Electronic address: helinok@vip.tom.com.
Lin
L
He
D056810:Acaricides / Q000494:pharmacology*; D000818:Animals; D043182:Carboxylesterase / Q000187:drug effects / Q000235:genetics*; D004351:Drug Resistance / Q000235:genetics; D005260:Female; D020440:Gene Duplication / Q000235:genetics; D015870:Gene Expression; D005796:Genes / Q000235:genetics; D008925:Mites / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D011422:Propionates / Q000494:pharmacology; D011722:Pyrethrins / Q000494:pharmacology; D060888:Real-Time Polymerase Chain Reaction
D056810:Acaricides; D011422:Propionates; D011722:Pyrethrins; C044267:fenpropathrin; D043182:Carboxylesterase; C572153:cyflumetofen
Carboxylesterase genes; Cyflumetofen; Fenpropathrin; Resistance; Tetranychus cinnabarinus