Ultrastructural observation of oocytes in six types of stony corals.
In this study, the ultrastructure of the oocytes of 6 types of scleractinian corals was observed by transmission electron microscopy (TEM). Moreover, histological and ultrastructural analyses were performed to improve our understanding of the organelles involved in coral oocyte formation. In all 6 stony coral species, the microvilli were tubular and directly grew from the surface of the oocyte membrane; yolk bodies, lipid granules, and cortical alveoli accounted for most of the volume inside the oocytes, suggesting that they are associated with energy storage and buoyancy. Clear differences were observed in the size of yolk bodies and lipid granules in the oocytes of the 6 stony coral species, which occupied approximately 55%-80% of the inner space of the oocytes. Galaxea fascicularis exhibited the largest lipid granule volume, but the oocytes contained only an average number of 12.45 lipid granules per unit area. Only Montipora incrassata oocytes contained symbiotic algae. The smallest size and proportion of lipid granules in M. incrassata oocytes may be attributed to the presence of symbiotic algae and large yolk bodies, which may help oocytes produce energy and function as a nutritional source. This study is crucial for improving the understanding of the basic biology of coral reproduction, and the ensuing datasets is critical for conservation-oriented studies seeking to cryopreserve corals during these times of dramatic global climate change.
Department of Biotechnology, Mingdao University, Peetow, Chang Hua, Taiwan; Department of Post Modern Agriculture, Mingdao University, Peetow, Chang Hua, Taiwan.
Sujune
S
Tsai
Institute of Marine Biology, National Dong Hwa University, Checheng, Pingtung, Taiwan.
Wei-Chieh
WC
Chang
Reef Biology Research Group, Department of Marine Science, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.
Suchana
S
Chavanich
Reef Biology Research Group, Department of Marine Science, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.
Voranop
V
Viyakarn
National museum of Marine Biology & Aquarium, Checheng, Pingtung, Taiwan; Institute of Marine Biology, National Dong Hwa University, Checheng, Pingtung, Taiwan. Electronic address: chiahsin@nmmba.gov.tw.
Chiahsin
C
Lin
D000818:Animals; D037421:Anthozoa / Q000254:growth & development / Q000648:ultrastructure*; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D005260:Female; D046529:Microscopy, Electron, Transmission; D009865:Oocytes / Q000254:growth & development / Q000648:ultrastructure*; D010053:Ovary / Q000254:growth & development / Q000648:ultrastructure*; D012098:Reproduction / Q000502:physiology
Hard coral; Oocyte; TEM; Ultrastructure; Yolk formation