In silico cloning and B/T cell epitope prediction of triosephosphate isomerase from Echinococcus granulosus.
Cystic echinococcosis is a worldwide zoonosis caused by Echinococcus granulosus. Because the methods of diagnosis and treatment for cystic echinococcosis were limited, it is still necessary to screen target proteins for the development of new anti-hydatidosis vaccine. In this study, the triosephosphate isomerase gene of E. granulosus was in silico cloned. The B cell and T cell epitopes were predicted by bioinformatics methods. The cDNA sequence of EgTIM was composition of 1094 base pairs, with an open reading frame of 753 base pairs. The deduced amino acid sequences were composed of 250 amino acids. Five cross-reactive epitopes, locating on 21aa-35aa, 43aa-57aa, 94aa-107aa, 115-129aa, and 164aa-183aa, could be expected to serve as candidate epitopes in the development of vaccine against E. granulosus. These results could provide bases for gene cloning, recombinant expression, and the designation of anti-hydatidosis vaccine.
Department of Pathogenic Biology, Chongqing Medical University, Chongqing, 400016, China.
Fen
F
Wang
Department of Pathogenic Biology, Chongqing Medical University, Chongqing, 400016, China. yebina@sohu.com.|Research Center for Molecule Medicine and Tumor, Chongqing Medical University, Chongqing, China. yebina@sohu.com.
Bin
http://orcid.org/0000-0003-4174-9133
B
Ye
D000595:Amino Acid Sequence; D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D003001:Cloning, Molecular; D019295:Computational Biology; D018076:DNA, Complementary / Q000235:genetics; D004443:Echinococcosis / Q000276:immunology* / Q000469:parasitology; D048209:Echinococcus granulosus / Q000201:enzymology* / Q000235:genetics / Q000276:immunology*; D018985:Epitopes, B-Lymphocyte / Q000235:genetics / Q000276:immunology; D018984:Epitopes, T-Lymphocyte / Q000235:genetics / Q000276:immunology; D006801:Humans; D008961:Models, Structural; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D011499:Protein Processing, Post-Translational; D021382:Protein Sorting Signals; D021381:Protein Transport; D016415:Sequence Alignment; D014305:Triose-Phosphate Isomerase / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
D000947:Antigens, Helminth; D018076:DNA, Complementary; D018985:Epitopes, B-Lymphocyte; D018984:Epitopes, T-Lymphocyte; D021382:Protein Sorting Signals; D014305:Triose-Phosphate Isomerase
Bioinformatics; Echinococcus granulosus; Epitopes; In silico cloning; Triosephosphate isomerase