Protective effect of Eruca sativa seed oil against oral nicotine induced testicular damage in rats.
Nicotine is a pharmacologically active component of the tobacco that adversely affects the male reproductive system and fertility. Nicotine administration in experimental animals was found to affect spermatogenesis, epididymal sperm count, motility and the fertilizing potential of sperms. The goal of this work is to assess the protective or ameliorative effect of Eruca Sativa seed oil against testicular damage induced by oral administration of nicotine in rats. Male adult Sprague-Dawley rats were used and divided into three groups; control, nicotine treated and nicotine and Eruca seed oil treated groups. After three weeks of treatment, the rats were weighed and sacrificed where testes were removed and weighed then calculating relative testis weights. The testes were processed for routine paraffin embedding and staining and the sections were examined for different morphometric and histopathological changes. The results show that nicotine administration had an effect on the body and testis weight and various morphometric parameters of the testis. It also induced varying degrees of structural damage to the seminiferous tubules, with shrinkage and absence of mature spermatids. Disorganized, vacuolization and loss of germinal cells were noticed in the basement membrane. The co-administration of Eruca Sativa seed oil led to improvement in the morphometric and histopathological changes of the seminiferous tubules. In conclusion, Eruca Sativa seed oil treatment in this study had a protective role by reversing, almost completely, all morphometric and histological changes in the testis induced by nicotine administration.
Department of Anatomy, Faculty of Medicine, King Abdulaziz University, PO Box 80205, Jeddah 21589, Saudi Arabia.
Gamal Said
GS
Abd El-Aziz
Department of Anatomy, Faculty of Medicine, King Abdulaziz University, PO Box 80205, Jeddah 21589, Saudi Arabia; Department of Anatomy, Faculty of Medicine, Suez Canal University, Egypt.
Magdy Omar
MO
El-Fark
Department of Anatomy, Faculty of Medicine, King Abdulaziz University, PO Box 80205, Jeddah 21589, Saudi Arabia. Electronic address: raidhamdy@hotmail.com.
Raid Mahmoud
RM
Hamdy
D000284:Administration, Oral; D000818:Animals; D019607:Brassicaceae / Q000737:chemistry*; D004822:Epididymis / Q000187:drug effects / Q000293:injuries / Q000473:pathology; D006801:Humans; D008297:Male; D009538:Nicotine / Q000633:toxicity; D010938:Plant Oils / Q000008:administration & dosage*; D051381:Rats; D012639:Seeds / Q000737:chemistry; D013081:Sperm Motility / Q000187:drug effects; D013091:Spermatogenesis / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects / Q000473:pathology; D013737:Testis / Q000187:drug effects* / Q000293:injuries / Q000473:pathology
D010938:Plant Oils; D009538:Nicotine
Histology; Morphometry; Nicotine; Rat; Testis