Biosynthesis of isoxazolin-5-one and 3-nitropropanoic acid containing glucosides in juvenile Chrysomelina.
Stable-isotope-labeled precursors were used to establish the biosynthetic pathway leading from β-alanine towards isoxazolin-5-one glucoside 1 and its 3-nitropropanoate (3-NPA) ester 2 in Chrysomelina larvae. Both structural elements originate from sequestered plant-derived β-alanine or from propanoyl-CoA that is derived from the degradation of some essential amino acids, e.g. valine. β-Alanine is converted into 3-NPA and isoxazolinone 5 by consecutive oxidations of the amino group of β-Ala. Substituting the diphospho group of α-UDP-glucose with 5 generates the isoxazolin-5-one glucoside 1, which serves in the circulating hemolymph of the larva as a platform for esterification with 3-nitropropanoyl-CoA. The pathway was validated with larvae of Phaedon cochleariae, Chrysomela populi as well as Gastrophysa viridula.
Max Planck Institute for Chemical Ecology, Bioorganic Chemistry, Hans-Knoell-Straße 8, D-07745, Jena, Germany. boland@ice.mpg.de.
Tobias
T
Becker
Kerstin
K
Ploss
Wilhelm
W
Boland
D000818:Animals; D001517:Coleoptera / Q000737:chemistry / Q000378:metabolism*; D005960:Glucosides / Q000737:chemistry / Q000378:metabolism*; D007555:Isoxazoles / Q000737:chemistry / Q000378:metabolism*; D008968:Molecular Conformation; D009574:Nitro Compounds / Q000737:chemistry / Q000378:metabolism*; D011422:Propionates / Q000737:chemistry / Q000378:metabolism*
D005960:Glucosides; D007555:Isoxazoles; D009574:Nitro Compounds; D011422:Propionates; C013076:isoxazolin-5-one; C015392:3-nitropropionic acid