Modification biological activity of S and R forms of Proteus mirabilis and Burkholderia cepacia lipopolysaccharides by carrageenans.
The modification of biological features of S and R forms of Proteus mirabilis and Burkholderia cepacia LPS by kappa/iota and kappa/beta carrageenans was shown in Limulus activation test, ELISA, human complement activation and apoptotic assay. The role of positively charged substituent Ara4N in lipid A was evaluated as a suspected major domain for interactions with sulphate groups of carrageenans.The experiments obtained by three serological methods indicated that not only lipid A part of LPS but also polysaccharide elements such as core and O-specific chain are involved in interaction with carrageenes. Carrageenans turned out to be non-cytotoxic for A549 cells and were able to inhibit the apoptotic effect caused by lipid A of P. mirabilis and B. cepacia.
Department of Microbiology, Jan Kochanowski University, Świętokrzyska 15, 25-406, Kielce, Poland. Electronic address: arabski@ujk.kielce.pl.
Michał
M
Arabski
Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, 159, Pr. 100-letiya, 690022 Vladivostok, Russia.
Anna
A
Barabanova
Department of Microbiology, Jan Kochanowski University, Świętokrzyska 15, 25-406, Kielce, Poland.
Katarzyna
K
Gałczyńska
Department of Radiobiology and Immunology, Jan Kochanowski University, Świętokrzyska 15, 25-406, Kielce, Poland.
Aneta
A
Węgierek-Ciuk
Department of Microbiology, Jan Kochanowski University, Świętokrzyska 15, 25-406, Kielce, Poland.
Kamila
K
Dzidowska
Department of Pathogen Biology and Immunology, University of Wrocław, Przybyszewskiego 63/77, 51-148 Wrocław, Poland.
Daria
D
Augustyniak
Department of Pathogen Biology and Immunology, University of Wrocław, Przybyszewskiego 63/77, 51-148 Wrocław, Poland.
Zuzanna
Z
Drulis-Kawa
Department of Radiobiology and Immunology, Jan Kochanowski University, Świętokrzyska 15, 25-406, Kielce, Poland; Institute of Chemistry and Nuclear Technology, Dorodna 16, 03-195 Warszawa, Poland.
Anna
A
Lankoff
Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, 159, Pr. 100-letiya, 690022 Vladivostok, Russia.
Irina
I
Yermak
Department of Chemical Sciences, University of Naples Federico II, Complesso Universitario Monte Sant' Angelo, Via Cinthia 4, 80126 Napoli, Italy.
Antonio
A
Molinaro
Department of Microbiology, Jan Kochanowski University, Świętokrzyska 15, 25-406, Kielce, Poland.
Wiesław
W
Kaca
D000072283:A549 Cells; D017209:Apoptosis / Q000187:drug effects; D016956:Burkholderia cepacia / Q000737:chemistry*; D002351:Carrageenan / Q000737:chemistry*; D003165:Complement System Proteins / Q000378:metabolism; D006801:Humans; D008050:Lipid A / Q000378:metabolism; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D011513:Proteus mirabilis / Q000737:chemistry*; D013237:Stereoisomerism
D008050:Lipid A; D008070:Lipopolysaccharides; D002351:Carrageenan; D003165:Complement System Proteins
Apoptosis; Burkholderia; Carrageenan; Carrageenan kappa (PubChem CID: 11966249) with iota (PubChem CID: 101231952) or beta (PubChem CID: 102199626); LAL; Lipopolysaccharide; Proteus