European official control of food: Determination of histamine in fish products by a HPLC-UV-DAD method.
The evaluation of histamine content in fish and fishery products, responsible for scombroid poisoning, is essential to guarantee the safety of food. EU regulation requires validated analytical methods to ensure the verification of compliance with food law in official control activity. To this aim a previous gradient RP-HPLC method with DAD detection was modified and validated, according to international guidelines. The reliability of results was tested by analysing fish reference materials within the participation in European proficiency tests. The method has been used for the analysis of real samples consisting of several fish-based products with considerable differences in matrix composition. This characteristic is of great relevance to be able of apply the method in the field of official control.
Veterinary Public Health and Food Safety Department, Food Chemical Safety and Human Health Unit, Istituto Superiore di Sanità, Rome, Italy.
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Altieri
Veterinary Public Health and Food Safety Department, Food Chemical Safety and Human Health Unit, Istituto Superiore di Sanità, Rome, Italy. Electronic address: antonella.semeraro@iss.it.
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Semeraro
Veterinary Public Health and Food Safety Department, Food Chemical Safety and Human Health Unit, Istituto Superiore di Sanità, Rome, Italy.
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Scalise
Veterinary Public Health and Food Safety Department, Food Chemical Safety and Human Health Unit, Istituto Superiore di Sanità, Rome, Italy.
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Calderari
Veterinary Public Health and Food Safety Department, Food Chemical Safety and Human Health Unit, Istituto Superiore di Sanità, Rome, Italy.
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Stacchini
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005396:Fish Products / Q000032:analysis*; D006632:Histamine / Q000032:analysis*; D015203:Reproducibility of Results
D006632:Histamine
EU regulation; Fish; HPLC; Histamine; Official control in food; Validation