A click chemistry-based microRNA maturation assay optimized for high-throughput screening.
Catalytic enzyme-linked click-chemistry assays (cat-ELCCA) are an emerging class of biochemical assay. Herein we report on expanding the toolkit of cat-ELCCA to include the kinetically superior inverse-electron demand Diels-Alder (IEDDA) reaction. The result is a technology with improved sensitivity and reproducibility, enabling automated high-throughput screening.
Program in Chemical Biology, University of Michigan, 210 Washtenaw Avenue, Ann Arbor, Michigan 48109, USA.
Daniel A
DA
Lorenz
Amanda L
AL
Garner
D055162:Biocatalysis; D057930:Click Chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D059014:High-Throughput Nucleotide Sequencing; D035683:MicroRNAs / Q000737:chemistry* / Q000378:metabolism
D035683:MicroRNAs