Renal denervation improves cardiac function in rats with chronic heart failure: Effects on expression of β-adrenoceptors.
Chronic activation of the sympathetic drive contributes to cardiac remodeling and dysfunction during chronic heart failure (HF). The present study was undertaken to assess whether renal denervation (RDN) would abrogate the sympathoexcitation in HF and ameliorate the adrenergic dysfunction and cardiac damage. Ligation of the left coronary artery was used to induce HF in Sprague-Dawley rats. Four weeks after surgery, RDN was performed, 1 wk before the final measurements. At the end of the protocol, cardiac function was assessed by measuring ventricular hemodynamics. Rats with HF had an average infarct area >30% of the left ventricle and left ventricular end-diastolic pressure (LVEDP) >20 mmHg. β1- and β2-adrenoceptor proteins in the left ventricle were reduced by 37 and 49%, respectively, in the rats with HF. RDN lowered elevated levels of urinary excretion of norepinephrine and brain natriuretic peptide levels in the hearts of rats with HF. RDN also decreased LVEDP to 10 mmHg and improved basal dP/dt to within the normal range in rats with HF. RDN blunted loss of β1-adrenoceptor (by 47%) and β2-adrenoceptor (by 100%) protein expression and improved isoproterenol (0.5 μg/kg)-induced increase in +dP/dt (by 71%) and -dP/dt (by 62%) in rats with HF. RDN also attenuated the increase in collagen 1 expression in the left ventricles of rats with HF. These findings demonstrate that RDN initiated in chronic HF condition improves cardiac function mediated by adrenergic agonist and blunts β-adrenoceptor expression loss, providing mechanistic insights for RDN-induced improvements in cardiac function in the HF condition.
Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, Nebraska.
Hong
H
Zheng
Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, Nebraska.
Xuefei
X
Liu
Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, Nebraska.
Neeru M
NM
Sharma
Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, Nebraska kpatel@unmc.edu.
Kaushik P
KP
Patel
D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D002908:Chronic Disease; D024042:Collagen Type I / Q000378:metabolism; D003331:Coronary Vessels / Q000601:surgery; D006321:Heart / Q000187:drug effects; D006333:Heart Failure / Q000209:etiology / Q000503:physiopathology*; D006352:Heart Ventricles / Q000378:metabolism / Q000503:physiopathology*; D007545:Isoproterenol / Q000494:pharmacology; D007672:Kidney Cortex / Q000378:metabolism; D008026:Ligation; D008297:Male; D009203:Myocardial Infarction / Q000150:complications; D020097:Natriuretic Peptide, Brain / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D018342:Receptors, Adrenergic, beta-1 / Q000378:metabolism; D018343:Receptors, Adrenergic, beta-2 / Q000378:metabolism; D012077:Renal Artery / Q000294:innervation*; D012964:Sodium / Q000652:urine; D013562:Sympathectomy
D000318:Adrenergic beta-Agonists; D024042:Collagen Type I; D018342:Receptors, Adrenergic, beta-1; D018343:Receptors, Adrenergic, beta-2; D020097:Natriuretic Peptide, Brain; D012964:Sodium; D007545:Isoproterenol; D009638:Norepinephrine
cardiac function; heart failure; renal nerve; sympathetic nerve activity