Carotenoids and amphibians: effects on life history and susceptibility to the infectious pathogen, Batrachochytrium dendrobatidis.
Carotenoids are considered beneficial nutrients because they provide increased immune capacity. Although carotenoid research has been conducted in many vertebrates, little research has been done in amphibians, a group that is experiencing global population declines from numerous causes, including disease. We raised two amphibian species through metamorphosis on three carotenoid diets to quantify the effects on life-history traits and post-metamorphic susceptibility to a fungal pathogen (Batrachochytrium dendrobatidis; Bd). Increased carotenoids had no effect on survival to metamorphosis in gray treefrogs (Hyla versicolor) but caused lower survival to metamorphosis in wood frogs [Lithobates sylvaticus (Rana sylvatica)]. Increased carotenoids caused both species to experience slower development and growth. When exposed to Bd after metamorphosis, wood frogs experienced high mortality, and the carotenoid diets had no mitigating effects. Gray treefrogs were less susceptible to Bd, which prevented an assessment of whether carotenoids could mitigate the effects of Bd. Moreover, carotenoids had no effect on pathogen load. As one of only a few studies examining the effects of carotenoids on amphibians and the first to examine potential interactions with Bd, our results suggest that carotenoids do not always serve amphibians in the many positive ways that have become the paradigm in other vertebrates.
Department of Biological Sciences, Southwestern Oklahoma State University, Weatherford, OK 73096, USA.
Rickey D
RD
Cothran
Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA.
Stephanie S
SS
Gervasi
Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA.
Cindy
C
Murray
Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA.
Beverly J
BJ
French
Environmental Sciences Graduate Program, Oregon State University, Corvallis, OR 97331, USA.
Paul W
PW
Bradley
Environmental Sciences Graduate Program, Oregon State University, Corvallis, OR 97331, USA.
Jenny
J
Urbina
Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA.
Andrew R
AR
Blaustein
Department of Biological Sciences, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
Rick A
RA
Relyea
Amphibian decline; disease ecology; nutritional ecology; parasite; pathogen