[Seminal plasma miR-122-3p and miR-141-5p stability and its diagnosis value for idiopathic asthenospermia].
OBJECTIVE
To explore the stability of seminal plasma miR-122-3p and miR-141-5p and their diagnostic value for idiopathic asthenospermia.


METHODS
Seminal plasma miR-122-3p and miR-141-5p with various incubation time, freeze-thaw cycles and incubation time were analyzed. Real-time fluorescence quantitative polymerase chain reaction (RT-PCR) was used to detect the content of miR-122-3p and miR-141-5p, with U6 snRNA as the reference. The idiopathic asthenospermia group was further divided into grade I and II based on the progressive motility percent, and the content of miR-122-3p and miR-141-5p in the two grades were compared.


RESULTS
No difference was detected after incubation at room temperature and 4 degrees Celsius. But seminal plasma miR-122-3p and miR-141-5p have decreased along with the increase in freeze-thaw cycles increases. RT-PCR assayed showed that the miR-122-3p content in the idiopathic asthenospermia group was significantly lower than the control group, while miR-141-5p was significantly higher. The difference in both miR-122-3p and miR-141-5p content was statistically significant (P < 0.05). A significant difference in miR-122-3p and miR-141-5p content between a and b groups was also detected (P < 0.05). The AUC-ROC of miR-122-3p was 0.88. At the cutoff value of 1.02, the sensitivity and specificity was 83% and 84%, respectively. At the cutoff of 2.95, the AUC-ROC of miR-141-5p was 0.88. The sensitivity and specificity was 84% and 70%, respectively.


CONCLUSIONS
MiR-122-3p and miR-141-5p in seminal plasma are stable and have certain value for the diagnosis of idiopathic asthenospermia.
Ningbo Hospital of Traditional Chinese Medicine Affiliated to Zhejiang University of Traditional Chinese Medicine, Ningbo, Zhejiang 315012, China. nbxiaowei@163.com.
Xiaoxia
X
Zhang
Renxiong
R
Wei
Jiangtao
J
Lou
Jun
J
Zhou
D000328:Adult; D053627:Asthenozoospermia / Q000175:diagnosis* / Q000235:genetics; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000032:analysis* / Q000737:chemistry; D008875:Middle Aged; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction; D012661:Semen / Q000737:chemistry*
C531907:MIRN122 microRNA, human; C533895:MIRN141 microRNA, human; D035683:MicroRNAs