Blu-ray Technology-Based Quantitative Assays for Cardiac Markers: From Disc Activation to Multiplex Detection.
Acute myocardial infarction (AMI) is the leading cause of mortality and morbidity globally. To reduce the number of mortalities, reliable and rapid point-of-care (POC) diagnosis of AMI is extremely critical. We herein present a Blu-ray technology-based assay platform for multiplex cardiac biomarker detection; not only off-the-shelf Blu-ray discs (BDs) were adapted as substrates to prepare standard immunoassays and DNA aptamer/antibody hybrid assays for the three key cardiac marker proteins (myoglobin, troponin I, and C-creative protein) but also an unmodified optical drive was directly employed to read the assay results digitally. In particular, we have shown that all three cardiac markers can be quantitated in their respective physiological ranges of interest, and the detection limits achieved are comparable with conventional enzyme-linked immunosorbent assay (ELISA) kits. The Blu-ray assay platform was further validated by measuring real-world samples and establishing a linear correlation with the simultaneously obtained ELISA data. Without the need to modify either the hardware (Blu-ray discs and optical drives) or the software driver, this assay-on-a-BD technique promises to be a low-cost user-friendly quantitative tool for on-site chemical analysis and POC medical diagnosis.
Department of Chemistry, Simon Fraser University , Burnaby, British Columbia V5A 1S6, Canada.
Samuel
S
Weng
Key Laboratory of Advanced Transducers and Intelligent Control Systems (Ministry of Education and Shanxi Province), College of Physics and Optoelectronics, Taiyuan University of Technology , Shanxi 030024, P. R. China.
Xiaochun
X
Li
eSenso Biotech Inc. , 308-2999 Underhill Avenue, Burnaby, British Columbia V5A 3C2, Canada.
Michelle
M
Niu
eSenso Biotech Inc. , 308-2999 Underhill Avenue, Burnaby, British Columbia V5A 3C2, Canada.
Bixia
B
Ge
Department of Chemistry, Simon Fraser University , Burnaby, British Columbia V5A 1S6, Canada.|Key Laboratory of Advanced Transducers and Intelligent Control Systems (Ministry of Education and Shanxi Province), College of Physics and Optoelectronics, Taiyuan University of Technology , Shanxi 030024, P. R. China.
Hua-Zhong
HZ
Yu
D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D052157:Aptamers, Nucleotide / Q000737:chemistry; D015415:Biomarkers / Q000032:analysis*; D002097:C-Reactive Protein / Q000032:analysis; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D057230:Limit of Detection; D009203:Myocardial Infarction / Q000175:diagnosis*; D009211:Myoglobin / Q000032:analysis; D019095:Point-of-Care Systems; D019210:Troponin I / Q000032:analysis
D000906:Antibodies; D052157:Aptamers, Nucleotide; D015415:Biomarkers; D009211:Myoglobin; D019210:Troponin I; D002097:C-Reactive Protein