Single-Nucleotide Polymorphisms and Markers of Oxidative Stress in Healthy Women.
OBJECTIVE
There is accumulating evidence that oxidative stress is an important contributor to carcinogenesis. We hypothesized that genetic variation in genes involved in maintaining antioxidant/oxidant balance would be associated with overall oxidative stress.


METHODS
We examined associations between single nucleotide polymorphisms (SNPs) in MnSOD, GSTP1, GSTM1, GPX1, GPX3, and CAT genes and thiobarbituric acid-reactive substances (TBARS), a blood biomarker of oxidative damage, in healthy white women randomly selected from Western New York (n = 1402). We used general linear models to calculate age-adjusted geometric means of TBARS across the variants. We also examined the associations within strata of menopausal status.


RESULTS
For MnSOD, being heterozygous was associated with lower geometric means of TBARS (less oxidative stress), 1.28 mg/dL, compared to homozygous T-allele or homozygous C-allele,1.35 mg/dL, and 1.31 mg/dL correspondingly (p for trend = 0.01). This difference remained among postmenopausal women, 1.40 mg/dL for TT, 1.32 mg/dL for TC, and 1.34mg/dL for CC (p for trend 0.015); it was attenuated among premenopausal women. SNPs in the other genes examined (GSTP1, GSTM1, GPX1, GPX3, and CAT) were not associated with TBARS.


CONCLUSIONS
Our findings suggest that genetic variation in MnSOD gene may be associated with oxidative status, particularly among postmenopausal women.
Department of Epidemiology and Environmental Health, University at Buffalo, Buffalo, New York, United States of America.
Albina N
AN
Minlikeeva
Department of Biotechnical and Clinical Laboratory Sciences, University at Buffalo, Buffalo, New York, United States of America.
Richard W
RW
Browne
Department of Epidemiology and Environmental Health, University at Buffalo, Buffalo, New York, United States of America.
Heather M
HM
Ochs-Balcom
The Ohio State University Comprehensive Cancer Center, Division of Cancer Prevention and Control, Columbus, Ohio, United States of America.|Victor Babes University of Medicine and Pharmacy, Timisoara, Romania.
Catalin
C
Marian
The Ohio State University Comprehensive Cancer Center, Division of Cancer Prevention and Control, Columbus, Ohio, United States of America.
Peter G
PG
Shields
Sophie Davis School of Biomedical Education, City College of New York, New York, New York, United States of America.
Maurizio
M
Trevisan
The Ohio State University Comprehensive Cancer Center, Division of Cancer Prevention and Control, Columbus, Ohio, United States of America.
Shiva
S
Krishnan
BioServe Biotechnologies Ltd., Beltsville, Maryland, United States of America.
Ramakrishna
R
Modali
BioServe Biotechnologies Ltd., Beltsville, Maryland, United States of America.
Michael
M
Seddon
BioServe Biotechnologies Ltd., Beltsville, Maryland, United States of America.
Teresa
T
Lehman
Department of Epidemiology and Environmental Health, University at Buffalo, Buffalo, New York, United States of America.
Jo L
JL
Freudenheim
D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D008875:Middle Aged; D018384:Oxidative Stress / Q000235:genetics*; D010088:Oxidoreductases / Q000097:blood* / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism*
D017392:Thiobarbituric Acid Reactive Substances; D010088:Oxidoreductases