Multiplexed detection of lung cancer biomarkers based on quantum dots and microbeads.
We have developed a multiplexed fluoroimmunoassay of three lung cancer biomarkers based on multicolor quantum dots (QDs) as detection elements and micro-magnetic beads as immune carriers. QDs have the ability to simplify multiplexed analysis. In our method, the fluorescent signals derived from three cross-talk-free QD conjugated probes with emission maxima at 525, 585 and 625nm could be analyzed to determine the concentrations of the target proteins. With this system, fragments of cytokeratin 19 (CYRFA 21-1), carcinoembryonic antigen (CEA), and neuron-specific enolase (NSE), were simultaneously detected in a single sample with a low detection limit down to the 1.0ng/mL level (364pg/mL for CYRFA 21-1, 38pg/mL for CEA, 370pg/mL for NSE in a single detection). Additional advantages of the presented method include ease of operation, low cost, and a very low sample volume (20µL).
State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, 865 Changning Road, Shanghai 200050, China; School of Life Science, East China Normal University, Shanghai 200241, China.
Simin
S
Wu
State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, 865 Changning Road, Shanghai 200050, China.
Lifen
L
Liu
State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, 865 Changning Road, Shanghai 200050, China.
Gong
G
Li
State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, 865 Changning Road, Shanghai 200050, China.
Fengxiang
F
Jing
State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, 865 Changning Road, Shanghai 200050, China.
Hongju
H
Mao
State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, 865 Changning Road, Shanghai 200050, China.
Qinghui
Q
Jin
Biomaterials and Tissue Engineering Research Center, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China.
Wanyin
W
Zhai
School of Life Science, East China Normal University, Shanghai 200241, China. Electronic address: hfzhang@bio.ecnu.edu.cn.
Hongfeng
H
Zhang
State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, 865 Changning Road, Shanghai 200050, China.
Jianlong
J
Zhao
State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, 865 Changning Road, Shanghai 200050, China. Electronic address: jiachp@mail.sim.ac.cn.
Chunping
C
Jia
D055910:Antibodies, Immobilized / Q000737:chemistry*; D014408:Biomarkers, Tumor / Q000032:analysis / Q000097:blood; D002272:Carcinoembryonic Antigen / Q000032:analysis / Q000097:blood*; D015200:Fluoroimmunoassay / Q000379:methods*; D006801:Humans; D053539:Keratin-19 / Q000032:analysis / Q000097:blood*; D057230:Limit of Detection; D008175:Lung Neoplasms / Q000097:blood* / Q000175:diagnosis; D010751:Phosphopyruvate Hydratase / Q000032:analysis / Q000097:blood*; D045663:Quantum Dots / Q000737:chemistry*
D055910:Antibodies, Immobilized; D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D053539:Keratin-19; D010751:Phosphopyruvate Hydratase
Biomarker; Immunoassay; Lung cancer; Multiplexed detection; Quantum dots