Ischemic-reperfusion of unilateral external iliac artery in rat: A new model for vasculitic femoral neuropathy.
Clinically, ischemic environment during gynecological surgery at lithotomy position is most common causative factor for the development of vasculitic femoral neuropathy (VFN). The present study was designed to induce the clinically relevant rat model of VFN by ischemic-reperfusion (I/R) injury of unilateral external iliac artery (uEIA). The VFN was induced by 3, 4 and 5h occlusion of uEIA followed by reperfusion. The I/R of uEIA induced VFN was evaluated by (i) behavioral parameters i.e., hind limb temperature; weight bearing capacity; (ii) kinematic analysis i.e., paw posture, splay angle, static sciatic index (SSI), and ankle-angle tests; (iii) evaluation of pain perception i.e., plantar and pin prick; (iv) serum biochemical estimation i.e., nitrate, lipid peroxidation, TNF-α and calcium level; (v) evaluation of motor and sensory nerve conduction velocity; and (vi) measurement of nerve fiber density. The 4 and 5h occlusion of uEIA has produced the potential changes in behavioral, functional, electrophysiological, biochemical and histopathological assessment. The 5h occlusion of uEIA has shown to produce the mortality. Whereas, 3h occlusion does not produce the significant changes in the development of VFN. The 4h ischemic occlusion of uEIA has shown potential rat model of VFN due to its close mimicking capacity of VFN in human. Therefore, it can be useful to explore the newer anti-neuralgic medicine and with their pharmacodynamic action in the field of various neurovascular disorders.
Department of Pharmacology, Neuropharmacology Division, Akal Toxicology Research Centre, A Unit of Akal College of Pharmacy & Technical Education, Mastuana Sahib, Sangrur 148001, Punjab, India. Electronic address: arunachalammu@gmail.com.
Arunachalam
A
Muthuraman
Department of Pharmacology, Neuropharmacology Division, Akal Toxicology Research Centre, A Unit of Akal College of Pharmacy & Technical Education, Mastuana Sahib, Sangrur 148001, Punjab, India; School of Health Sciences, University of KwaZulu-Natal (UKZN), Durban 4001 South Africa. Electronic address: muthusamyrameshniper@gmail.com.
Muthusamy
M
Ramesh
D000818:Animals; D001696:Biomechanical Phenomena; D004195:Disease Models, Animal; D020428:Femoral Neuropathy / Q000209:etiology / Q000503:physiopathology / Q000523:psychology; D007083:Iliac Artery / Q000293:injuries*; D008297:Male; D009431:Neural Conduction; D009437:Neuralgia / Q000209:etiology / Q000503:physiopathology / Q000523:psychology; D018384:Oxidative Stress; D058748:Pain Perception; D017288:Pain Threshold; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000150:complications / Q000503:physiopathology / Q000523:psychology
Ankle angle test; External iliac artery; Nerve conduction velocity; Splay angle; Vasculitic femoral neuropathy