Down-regulation of adenylate kinase 5 in temporal lobe epilepsy patients and rat model.
Adenylate kinase 5 (AK5) is one member of the AK family and plays a critical role in maintaining cellular homeostasis. Different from the other AKs, AK5 is almost exclusively expressed in the brain. However, its exact biological functions remain unclear. The aim of the present study is to explore the expression pattern of AK5 in patients with refractory epilepsy and in a chronic pilocarpine-induced epileptic rat model. Using Western blot, immunofluorescence and immunoprecipitation analysis, we found that AK5 protein was mainly expressed in neurons, demonstrated by colocalization with the dendritic marker, MAP2, which were similar to the corresponding controls. However, the expression of AK5 decreased remarkably in epileptic patients and experimental rats. Furthermore, immunoprecipitation analysis showed that the interaction of AK5 with copine VI (CPNE6, a brain specific protein) increased in epileptic patients and rat models. Our results are the first to indicate that the expression of AK5 in epileptic brain tissue may play important roles in epilepsy, especially refractory epilepsy.
Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Neurology, 1 Youyi Road, Chongqing 400016, China; Department of Neurology, Affiliated Haikou Hospital of Xiangya Medical College of Central South University, Haikou Municipal Hospital, Haikou, Hainan 570208, China.
Yujie
Y
Lai
Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Neurology, 1 Youyi Road, Chongqing 400016, China.
Xiaotong
X
Hu
Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Neurology, 1 Youyi Road, Chongqing 400016, China.
Guojun
G
Chen
Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Neurology, 1 Youyi Road, Chongqing 400016, China; Center of Epilepsy, Beijing Institute for Brain Disorders, 10 Xitoutiao, Youanmen, Fengtai District, Beijing 100069, China.
Xuefeng
X
Wang
Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Neurology, 1 Youyi Road, Chongqing 400016, China. Electronic address: zhubinglin0311@163.com.
Binglin
B
Zhu
D000263:Adenylate Kinase / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D000818:Animals; D000070642:Brain Injuries, Traumatic / Q000201:enzymology / Q000601:surgery; D002352:Carrier Proteins / Q000378:metabolism; D002648:Child; D004195:Disease Models, Animal; D015536:Down-Regulation; D000069279:Drug Resistant Epilepsy / Q000201:enzymology* / Q000601:surgery; D004833:Epilepsy, Temporal Lobe / Q000201:enzymology* / Q000601:surgery; D005260:Female; D006801:Humans; D008297:Male; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009474:Neurons / Q000201:enzymology; D010862:Pilocarpine; D017207:Rats, Sprague-Dawley; D055815:Young Adult
C000616618:CPNE6 protein, human; C000616617:CPNE6 protein, rat; D002352:Carrier Proteins; C489787:MAP2 protein, rat; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D010862:Pilocarpine; D000263:Adenylate Kinase; C118782:adenylate kinase 5
Adenylate kinase 5 (AK5); Copine VI (CPNE6); Patients; Rat model; Refractory epilepsy