Identification and characterization of phenolic compounds in hydromethanolic extracts of sorghum wholegrains by LC-ESI-MS(n).
Hydromethanolic extracts of brown, red, and white sorghum whole grains were analysed by LC-MS(n) in negative ESI mode within the range m/z 150-550amu. Besides the flavonoids already reported in sorghum, a number of flavonoids were also identified in the sorghum grain for the first time, including flavanones, flavonols and flavanonols, and flavan-3-ol derivatives. Various phenylpropane glycerides were also found in the sorghum grain, the majority of them are reported here for the first time, and a few of them were detected with abundant peaks in the extracts, indicating they are another important class of phenolic compounds in sorghum. In addition, phenolamides were also found in sorghum grain, which have not been reported before, and dicaffeoyl spermidine was detected in high abundance in the extracts of all three type sorghum grains. These results confirmed that sorghum is a rich source of various phenolic compounds.
School of Chemistry, Faculty of Science, Medicine and Health, University of Wollongong, Wollongong, NSW 2522, Australia.
Jinguo
J
Kang
School of Chemistry, Faculty of Science, Medicine and Health, University of Wollongong, Wollongong, NSW 2522, Australia. Electronic address: wprice@uow.edu.au.
William E
WE
Price
Sanitarium Health and Wellbeing, PO Box 40, Cooranbong, NSW 2265, Australia.
John
J
Ashton
School of Medicine, Faculty of Science, Medicine and Health, University of Wollongong, Wollongong, NSW 2522, Australia.
Linda C
LC
Tapsell
School of Public Health, Faculty of Health Sciences, Curtin University, Perth, WA 6845, Australia.
Stuart
S
Johnson
D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D005419:Flavonoids / Q000032:analysis*; D044948:Flavonols / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D005989:Glycerides / Q000032:analysis; D013058:Mass Spectrometry; D064209:Phytochemicals / Q000032:analysis; D010936:Plant Extracts; D059808:Polyphenols / Q000032:analysis*; D045868:Sorghum / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013095:Spermidine / Q000032:analysis
D005419:Flavonoids; D044948:Flavonols; D005989:Glycerides; D064209:Phytochemicals; D010936:Plant Extracts; D059808:Polyphenols; C404987:flavan-3-ol; D013095:Spermidine
HPLC-ESI-MS(n); Phenolic compounds; Sorghum