Plasma cysteine/cystine redox couple disruption in animal models of temporal lobe epilepsy.
Currently the field of epilepsy lacks peripheral blood-based biomarkers that could predict the onset or progression of chronic seizures following an epileptogenic injury. Thiol/disulfide ratios have been shown to provide a sensitive means of assessing the systemic redox potential in tissue and plasma. In this study, we utilized a rapid, simple and reliable method for simultaneous determination of several thiol-containing amino acids in plasma using HPLC with electrochemical detection in kainic acid (KA) and pilocarpine rat models of epilepsy. In contrast to GSH and GSSG levels, the levels of cysteine (Cys) were decreased by 42% and 62% and cystine (Cyss) were increased by 46% and 23% in the plasma of KA- and pilocarpine-injected rats, respectively after 48h. In chronically epileptic rats, plasma cysteine was decreased by 40.4% and 37.7%, and plasma GSSG increased by 33.8% and 35.0% following KA and pilocarpine, respectively. Treatment of rats with a catalytic antioxidant, 60min after KA or pilocarpine significant attenuated the decrease of plasma Cys/Cyss ratios at the 48h time point in both models. These observations suggest that the decreased cysteine and ratio of Cys/Cyss in plasma could potentially serve as redox biomarkers in temporal lobe epilepsy.
Department of Pharmaceutical Sciences, University of Colorado Denver, Aurora, CO, Unites States.
Li-Ping
LP
Liang
Department of Pharmaceutical Sciences, University of Colorado Denver, Aurora, CO, Unites States. Electronic address: manisha.patel@ucdenver.edu.
Manisha
M
Patel
D000818:Animals; D015415:Biomarkers; D003545:Cysteine / Q000097:blood / Q000378:metabolism*; D004195:Disease Models, Animal; D004833:Epilepsy, Temporal Lobe / Q000097:blood / Q000378:metabolism*; D005978:Glutathione / Q000097:blood / Q000378:metabolism; D019803:Glutathione Disulfide / Q000097:blood / Q000378:metabolism; D008297:Male; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D051381:Rats; D013438:Sulfhydryl Compounds / Q000097:blood / Q000378:metabolism
D015415:Biomarkers; D013438:Sulfhydryl Compounds; D005978:Glutathione; D003545:Cysteine; D019803:Glutathione Disulfide
Biomarker; Catalytic antioxidant; Cysteine; Epilepsy; Glutathione; HPLC