Nitric oxide and asymmetric dimethyl arginine (ADMA) levels in an experimental hydronephrotic kidney caused by unilateral partial ureteral obstruction.
OBJECTIVE
Our aim is to measure asymmetric dimethyl arginine and nitric oxide levels in rats with induced unilateral acute ureteral obstruction to research the effects on the kidney.


METHODS
The study included 21 adolescent (average age 6 weeks) Sprague-Dawley male rats weighing between 240-290g divided at random into 3 groups. Group-1: Control group (n=6): underwent no procedures. Group-2: Sham group (n=6): underwent the same procedures as the experimental group without ureter and psoas muscle dissection. Group-3: Group with induced partial unilateral ureteral obstruction (n=9). All rats were sacrificed after 12 weeks. Superoxide dismutase enzyme activity and nitrite and nitrate salt levels were measured in renal tissue. Plasma nitrite-nitrate and ADMA levels were examined.


RESULTS
In the experimental group histopathological changes observed included renal pelvis dilatation, flattened papillae, sclerotic glomerulus and fibrosis. In the experimental group tissue SOD and blood ADMA levels were higher than the control and sham groups (p<0.05) while tissue NO and plasma NO values were lower than in the sham and control groups (p<0.05).


CONCLUSIONS
Oxidative stress and disruption of NO synthesis play an important role in renal function and histopathological changes after obstructive renal disease. To prevent renal complications developing after obstructive nephropathy we believe that a new strategy may be research on reducing ADMA.
Department of Urology, Medical Faculty, Canakkale Onsekiz Mart University, Turkey.
Cabir
C
Alan
Department of Urology, Medical Faculty, Canakkale Onsekiz Mart University, Turkey.
Hasan Anil
HA
Kurt
Department of Urology, Medical Faculty, Canakkale Onsekiz Mart University, Turkey.
Naci
N
Topaloğlu
Department of Urology, Medical Faculty, Canakkale Onsekiz Mart University, Turkey.
Ahmet Reşit
AR
Ersay
Department of Urology, Medical Faculty, Canakkale Onsekiz Mart University, Turkey.
Dilek Ulker
DU
Cakir
Department of Urology, Medical Faculty, Canakkale Onsekiz Mart University, Turkey.
Gokhan
G
Başturk
D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives* / Q000097:blood; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D006869:Hydronephrosis / Q000209:etiology* / Q000378:metabolism / Q000473:pathology*; D007668:Kidney / Q000473:pathology; D008297:Male; D009566:Nitrates / Q000032:analysis; D009569:Nitric Oxide / Q000032:analysis* / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology; D016612:Paraffin Embedding; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D013482:Superoxide Dismutase / Q000032:analysis; D014517:Ureteral Obstruction / Q000150:complications* / Q000378:metabolism
D009566:Nitrates; D009569:Nitric Oxide; C018524:N,N-dimethylarginine; D001120:Arginine; D013482:Superoxide Dismutase