Reconsideration of iodine in wound irrigation: the effects on Pseudomonas aeruginosa biofilm formation.
OBJECTIVE
Chronic skin wounds are usually colonised with bacteria and subsequent infection may develop. Topical antiseptics are commonly used to control bacterial colonisation. The topical antiseptic, 1% polyvinylpyrrolidone-iodine (PVP-I), that is used on chronic open skin wounds remains controversial in the clinical setting because of its cytotoxicity. Here, we tested 1% PVP-I solution against saline to determine if it reduces bacterial count on the wound surface and within the tissue that may lead to wound reduction.


METHODS
Open wounds that were created on the backs of Sprague Dawley rats were inoculated with Pseudomonas aeruginosa at the wound surface. Wounds were kept covered except for wound irrigation, two days post-wounding, wounds were irrigated daily using a 10ml syringe and spray tip.


RESULTS
Our results indicate that 1% PVP-I irrigation resulted in a reduced bacterial count on the wound surface and within the tissue compared with saline irrigation. The 1% PVP-I irrigation promoted wound re-epithelialisation compared with saline irrigation, but it did not reach significance.


CONCLUSIONS
These results indicated that irrigation with 1% PVP-I was an effective way to reduce bacterial count on the wound surface, and allow the wound to progress to healing.
Department of Science of Nursing Practice, Tohoku University Graduate School of Medicine, 2-1 Seiryo-cho, Aoba-ku, Sendai, Japan.
E
E
Kanno
Department of Plastic and Reconstructive Surgery, Tohoku University Graduate School of Medicine, 2-1 Seiryo-cho, Aoba-ku, Sendai, Japan.
H
H
Tanno
Department of Plastic and Reconstructive Surgery, Tohoku University Graduate School of Medicine, 2-1 Seiryo-cho, Aoba-ku, Sendai, Japan.
A
A
Suzuki
Department of Plastic and Reconstructive Surgery, Tohoku University Graduate School of Medicine, 2-1 Seiryo-cho, Aoba-ku, Sendai, Japan.
R
R
Kamimatsuno
Department of Plastic and Reconstructive Surgery, Tohoku University Graduate School of Medicine, 2-1 Seiryo-cho, Aoba-ku, Sendai, Japan.
M
M
Tachi
D000818:Animals; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D058491:Bacterial Load / Q000187:drug effects*; D018441:Biofilms / Q000187:drug effects*; D008297:Male; D011206:Povidone-Iodine / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012965:Sodium Chloride / Q000494:pharmacology; D007507:Therapeutic Irrigation / Q000379:methods*; D014947:Wounds and Injuries / Q000473:pathology / Q000628:therapy*
D000891:Anti-Infective Agents, Local; D012965:Sodium Chloride; D011206:Povidone-Iodine
1% PVP-I; Pseudomonas aeruginosa; biofilm; skin; wound healing