LC-MS Guided Isolation of Bioactive Principles from Iris hookeriana and Bioevaluation of Isolates for Antimicrobial and Antioxidant Activities.
The genus Iris is diverse both in the abundance of secondary metabolities as well as the biological activities. The rhizomes of Iris hookeriana exhibit significant anthelminthic activity against gastrointestinal nematodes of sheep. Although Iris hookeriana has been a subject of the study of so many phytochemical studies, yet we report some constituents for the first time from this plant using a different isolation approach. This manuscript presents the isolation, antimicrobial and antioxidant evaluation of bioactive principles from Iris hookeriana. LC-MS guided isolation technique was applied for the separation of target constituents. The isolates were characterised by spectral techniques and subjected to antioxidant evaluation by DPPH assay. Four compounds; resveratrol, resveratroloside, junipeginin C and isorhamnetin-3-O-neohesperidoside were isolated for the first time along with 3 known compounds viz piceid, irigenin and iridin from I. hookeriana using this approach. The antioxidant activity screening of the isolates revealed that all the 4 constituents isolated for the first time, have strong antioxidant potential with IC50 of 14.0 µg/ml (resveratroloside), 19.7 µg/ml (junipeginen C), 12.8 µg/ml (resveratrol) and 19.8 µg/ml (isorhamnetin-3-O-neohesperodoside). So it can be safely concluded that LC-MS guided isolation of chemical compounds from Iris hookeriana has furnished 4 antioxidant constituents. Thus Iris hookeriana can act as as a good source of wonder molecule resveratrol and its 2 glycosides, resveratrolside and piceid which upon hydrolysis can be converted into the parent drug resveratrol.
Bioorganic Chemistry Division, Indian Institute of Integrative Medicine (CSIR), Srinagar, Jammu and Kashmir, India.
B A
BA
Dar
Bioorganic Chemistry Division, Indian Institute of Integrative Medicine (CSIR), Srinagar, Jammu and Kashmir, India.
S H
SH
Lone
Department of Chemistry, University of Kashmir, Srinagar, Jammu and Kashmir, India.
W A
WA
Shah
Bioorganic Chemistry Division, Indian Institute of Integrative Medicine (CSIR), Srinagar, Jammu and Kashmir, India.
K A
KA
Bhat
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002853:Chromatography, Liquid; D044948:Flavonols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D031323:Iris Plant / Q000737:chemistry*; D007529:Isoflavones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000737:chemistry; D000077185:Resveratrol; D021241:Spectrometry, Mass, Electrospray Ionization; D013267:Stilbenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
C000592752:3,5,4'-trihydroxystilbene-4'-O-glucoside; D000900:Anti-Bacterial Agents; D000975:Antioxidants; D044948:Flavonols; D005960:Glucosides; D007529:Isoflavones; D010936:Plant Extracts; D013267:Stilbenes; C469309:isorhamnetin-3-O-neohesperidoside; C000614660:junipeginin C; D000077185:Resveratrol