Comparison of SPE, IFE, and FLC in Monitoring Patients with Multiple Myeloma After Autologous Stem Cell Transplantation.
Conventionally, serum protein electrophoresis (SPE) and serum immunofixation electrophoresis (IFE) are used as primary methods to diagnose and monitor multiple myeloma (MM). Recently, serum-free light chain (FLC) assay has been incorporated into hematological screening programs for myeloma. The purpose of this study is to compare the performance of the three methods in monitoring MM patients after autologous stem cell transplantation (ASCT). SPE, serum IFE and serum FLC assay were performed on 38 MM patients who underwent ASCT. In total, four patients had unexpected protein bands (UPBs) and 13 patients had relapsed after ASCT. Our results indicate that IFE is more sensitive than SPE and FLC assay in detection of UPBs and relapse. The results of IFE may provide useful information in advance of patient relapse.
Department of Oncology, The First Affiliated Hospital of Soochow University, Suzhou, China.|PREMED Key Laboratory for Precision Medicine, Soochow University, Suzhou, China.
Wei
W
Li
Department of Hematology, The First Affiliated Hospital of Soochow University, Shizi Street 188, Suzhou, 215006, China.|Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou, China.|Collaborative Innovation Center of Hematology, Soochow University, Suzhou, China.|Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, China.
Jia-Zi
JZ
Zhou
Department of Hematology, The First Affiliated Hospital of Soochow University, Shizi Street 188, Suzhou, 215006, China.|Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou, China.|Collaborative Innovation Center of Hematology, Soochow University, Suzhou, China.|Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, China.
Hui-Rong
HR
Chang
Department of Hematology, The First Affiliated Hospital of Soochow University, Shizi Street 188, Suzhou, 215006, China.|Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou, China.|Collaborative Innovation Center of Hematology, Soochow University, Suzhou, China.|Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, China.
Li-Jun
LJ
Dai
Department of Hematology, The First Affiliated Hospital of Soochow University, Shizi Street 188, Suzhou, 215006, China.|Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou, China.|Collaborative Innovation Center of Hematology, Soochow University, Suzhou, China.|Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, China.
Zi-Ling
ZL
Zhu
Department of Hematology, The First Affiliated Hospital of Soochow University, Shizi Street 188, Suzhou, 215006, China.|Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou, China.|Collaborative Innovation Center of Hematology, Soochow University, Suzhou, China.|Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, China.
Yu-Feng
YF
Feng
Department of Hematology, The First Affiliated Hospital of Soochow University, Shizi Street 188, Suzhou, 215006, China. gongfeiran@suda.edu.cn.|Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou, China. gongfeiran@suda.edu.cn.|Collaborative Innovation Center of Hematology, Soochow University, Suzhou, China. gongfeiran@suda.edu.cn.|Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, China. gongfeiran@suda.edu.cn.
Fei-Ran
FR
Gong
Department of Hematology, The First Affiliated Hospital of Soochow University, Shizi Street 188, Suzhou, 215006, China. wudepei@suda.edu.cn.|Jiangsu Institute of Hematology, The First Affiliated Hospital of Soochow University, Suzhou, China. wudepei@suda.edu.cn.|Collaborative Innovation Center of Hematology, Soochow University, Suzhou, China. wudepei@suda.edu.cn.|Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou, China. wudepei@suda.edu.cn.
De-Pei
DP
Wu
D001797:Blood Protein Electrophoresis / Q000379:methods*; D001798:Blood Proteins / Q000032:analysis*; D006403:Hematologic Tests / Q000379:methods*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D009101:Multiple Myeloma / Q000097:blood* / Q000601:surgery / Q000628:therapy; D012680:Sensitivity and Specificity; D033581:Stem Cell Transplantation / Q000009:adverse effects*; D014182:Transplantation, Autologous / Q000009:adverse effects*
D001798:Blood Proteins
ASCT; Multiple myeloma; SPE; Serum FLC ratio; Serum IFE