Reduction of dioxin-like toxicity in effluents by additional wastewater treatment and related effects in fish.
Efficiency of advanced wastewater treatment technologies to reduce micropollutants which mediate dioxin-like toxicity was investigated. Technologies compared included ozonation, powdered activated carbon and granular activated carbon. In addition to chemical analyses in samples of effluents, surface waters, sediments, and fish, (1) dioxin-like potentials were measured in paired samples of effluents, surface waters, and sediments by use of an in vitro biotest (reporter gene assay) and (2) dioxin-like effects were investigated in exposed fish by use of in vivo activity of the mixed-function, monooxygenase enzyme, ethoxyresorufin O-deethylase (EROD) in liver. All advanced technologies studied, based on degradation or adsorption, significantly reduced dioxin-like potentials in samples and resulted in lesser EROD activity in livers of fish. Results of in vitro and in vivo biological responses were not clearly related to quantification of targeted analytes by use of instrumental analyses.
Animal Physiological Ecology, University of Tübingen, Auf der Morgenstelle 5, D-72076 Tübingen, Germany. Electronic address: dianamaier.mt@gmail.com.
Diana
D
Maier
Masaryk University, Faculty of Science, RECETOX, Kamenice 5, 62500 Brno, Czech Republic. Electronic address: benisek.martin@gmail.com.
Martin
M
Benisek
Masaryk University, Faculty of Science, RECETOX, Kamenice 5, 62500 Brno, Czech Republic. Electronic address: blaha@recetox.muni.cz.
Ludek
L
Blaha
Department of Science and Technological Innovation (DISIT), Università del Piemonte Orientale "Amedeo Avogadro" -Alessandria, Novara, Vercelli, Via Michel 11, 15121 Alessandria, Italy. Electronic address: francesco.dondero@uniupo.it.
Francesco
F
Dondero
Department of Biomedical Veterinary Sciences and Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan, Canada; School of Biological Sciences, University of Hong Kong, Hong Kong, SAR, China; State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, China. Electronic address: JGiesy@aol.com.
John P
JP
Giesy
Animal Physiological Ecology, University of Tübingen, Auf der Morgenstelle 5, D-72076 Tübingen, Germany. Electronic address: heinz-r.koehler@uni-tuebingen.de.
Heinz-R
HR
Köhler
DVGW Water Technology Center, Karlsruher Straße 84, D-76139 Karlsruhe, Germany. Electronic address: doreen.richter@tzw.de.
Doreen
D
Richter
DVGW Water Technology Center, Karlsruher Straße 84, D-76139 Karlsruhe, Germany. Electronic address: marco.scheurer@tzw.de.
Marco
M
Scheurer
Animal Physiological Ecology, University of Tübingen, Auf der Morgenstelle 5, D-72076 Tübingen, Germany; Steinbeis Transfer-Center for Ecotoxicology and Ecophysiology, Blumenstraße 13, D-72108 Rottenburg, Germany. Electronic address: stz.oekotox@gmx.de.
Rita
R
Triebskorn
D000327:Adsorption; D000818:Animals; D001681:Biological Assay; D002606:Charcoal; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism*; D004147:Dioxins / Q000737:chemistry / Q000633:toxicity*; D005393:Fish Diseases / Q000139:chemically induced*; D005399:Fishes; D015971:Gene Expression Regulation, Enzymologic; D008099:Liver / Q000201:enzymology; D062065:Wastewater / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000032:analysis
D002606:Charcoal; D019363:Cytochrome P-450 CYP1A1; D004147:Dioxins; D062065:Wastewater; D014874:Water Pollutants, Chemical
Activated carbon; EROD; Ozonation; Reporter gene assay; Sewage