Hybrid kappa\lambda antibody is a new serological marker to diagnose autoimmune pancreatitis and differentiate it from pancreatic cancer.
The only generally accepted serological marker currently used for the diagnosis of autoimmune pancreatitis (AIP) is IgG4. Our aim was mainly to determine whether hybrid κ\λ antibody can help to diagnose AIP and to differentiate it from pancreatic cancer. We established an enzyme-linked immunosorbent assay (ELISA) system to measure the levels of hybrid κ\λ antibodies in human sera. Sera were obtained from 338 patients, including 61 with AIP, 74 with pancreatic cancer, 50 with acute pancreatitis, 40 with ordinary chronic pancreatitis, 15 with miscellaneous pancreatic diseases, and 98 with normal pancreas. Our study showed levels of hybrid κ\λ antibodies in the AIP group were significantly higher than in the non-AIP group (P < 0.001). The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) for the diagnosis of AIP were 80.3%, 91%, 66.2% and 95.5% respectively. Furthermore, the combined measurement of serum hybrid κ\λ antibody and IgG4 tended to increase the sensitivity although the difference was not statistically significant (90.2% vs. 78.7%, P = 0.08), compared to measurement of IgG4 alone. Our findings suggest that hybrid κ\λ antibody could be a new serological marker to diagnose AIP and differentiate it from pancreatic cancer.
National Center for Clinical Laboratories, Beijing Hospital, Beijing, People's Republic of China.|Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People's Republic of China.|Department of Clinical Laboratory, Qianfo Mountain Hospital of Shandong University, Jinan, People's Republic of China.
Mingju
M
Hao
China-Japan Friendship Hospital, Beijing, People's Republic of China.
Wenli
W
Li
National Center for Clinical Laboratories, Beijing Hospital, Beijing, People's Republic of China.|Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People's Republic of China.
Lang
L
Yi
Department of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, People's Republic of China.
Songlin
S
Yu
National Center for Clinical Laboratories, Beijing Hospital, Beijing, People's Republic of China.|Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People's Republic of China.
Gaowei
G
Fan
National Center for Clinical Laboratories, Beijing Hospital, Beijing, People's Republic of China.|Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People's Republic of China.
Tian
T
Lu
National Center for Clinical Laboratories, Beijing Hospital, Beijing, People's Republic of China.|Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People's Republic of China.
Xin
X
Yang
National Center for Clinical Laboratories, Beijing Hospital, Beijing, People's Republic of China.|Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People's Republic of China.
Guojing
G
Wang
National Center for Clinical Laboratories, Beijing Hospital, Beijing, People's Republic of China.|Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People's Republic of China.
Dong
D
Zhang
National Center for Clinical Laboratories, Beijing Hospital, Beijing, People's Republic of China.|Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People's Republic of China.
Jiansheng
J
Ding
National Center for Clinical Laboratories, Beijing Hospital, Beijing, People's Republic of China.
Kuo
K
Zhang
National Center for Clinical Laboratories, Beijing Hospital, Beijing, People's Republic of China.
Rui
R
Zhang
National Center for Clinical Laboratories, Beijing Hospital, Beijing, People's Republic of China.
Guigao
G
Lin
National Center for Clinical Laboratories, Beijing Hospital, Beijing, People's Republic of China.
Yanxi
Y
Han
National Center for Clinical Laboratories, Beijing Hospital, Beijing, People's Republic of China.
Lunan
L
Wang
National Center for Clinical Laboratories, Beijing Hospital, Beijing, People's Republic of China.|Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, People's Republic of China.
Jinming
J
Li
D000208:Acute Disease; D001327:Autoimmune Diseases / Q000175:diagnosis*; D003937:Diagnosis, Differential; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D007145:Immunoglobulin kappa-Chains / Q000276:immunology*; D007146:Immunoglobulin lambda-Chains / Q000276:immunology*; D010190:Pancreatic Neoplasms / Q000175:diagnosis*; D010195:Pancreatitis / Q000175:diagnosis*; D012680:Sensitivity and Specificity
D007074:Immunoglobulin G; D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains