Biocompatibility evaluation of tissue-engineered decellularized scaffolds for biomedical application.
Biomaterials based on seeding of cells on decellularized scaffolds have gained increasing interest in the last few years and suggested to serve as an alternative approach to bioengineer artificial organs and tissues for transplantation. The reaction of the host toward the decellularized scaffold and transplanted cells depends on the biocompatibility of the construct. Before proceeding to the clinical application step of decellularized scaffolds, it is greatly important to apply a number of biocompatibility tests in vitro and in vivo. This review describes the different methodology involved in cytotoxicity, pathogenicity, immunogenicity and biodegradability testing for evaluating the biocompatibility of various decellularized matrices obtained from human or animals.
Stem Cell Institute, Kangwon National University, Chuncheon, Gangwon 200-701, Korea; Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 151-742, South Korea; Adult Stem Cell Research Center, College of Veterinary Medicine, Seoul National University, Seoul 08826, South Korea.
Kamal Hany
KH
Hussein
Stem Cell Institute, Kangwon National University, Chuncheon, Gangwon 200-701, Korea; Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 151-742, South Korea; Adult Stem Cell Research Center, College of Veterinary Medicine, Seoul National University, Seoul 08826, South Korea.
Kyung-Mee
KM
Park
Adult Stem Cell Research Center, College of Veterinary Medicine, Seoul National University, Seoul 08826, South Korea; Institue of Veterinary Medicine, College of Veterinary Medicine, Kangwon National University, Chuncheon, Gangwon 200-701, South Korea.
Kyung-Sun
KS
Kang
Stem Cell Institute, Kangwon National University, Chuncheon, Gangwon 200-701, Korea; Institue of Veterinary Medicine, College of Veterinary Medicine, Kangwon National University, Chuncheon, Gangwon 200-701, South Korea; Harvard Stem Cell Institute, Renal Division, Brigham and Women's Hospital, Harvard Medical School, MA 02115, USA. Electronic address: woohm@kangwon.ac.kr.
Heung-Myong
HM
Woo
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000627:therapeutic use*; D017690:Cell Transplantation / Q000379:methods*; D006801:Humans; D054457:Tissue Scaffolds / Q000737:chemistry*
D001672:Biocompatible Materials
Biocompatibility; Biodegradability; Cytotoxicity; Decellularization; Immunogenicity; Scaffolds