Comparison between superficial and solid-state cultures of Isaria fumosorosea: conidial yields, quality and sensitivity to oxidant conditions.
Conidia production and quality from mycoinsecticides in solid-state cultures (SSC) are frequently inferred from superficial culture (SC) results. Both parameters were evaluated for two Isaria fumosorosea strains (ARSEF 3302 and CNRCB1), in SC and SSC, using culture media with the same chemical composition. For both strains, conidia production was higher in SC than SSC in terms of conidia per gram of dry substrate. Germination in both strains did not show significant differences between SC and SSC (>90 %). Similarly, conidia viability in ARSEF 3302 strain did not show differences at early stages between SC and SSC, but was higher in SC compared to SSC in the late stage of culture; in contrast, conidia from CNRCB1 strain did not differ between both culture systems. Some infectivity parameters improved in conidia from SSC, compared to SC at the early stages, but these differences disappeared at the final stage, independently of the strain. Both strains showed decreased conidia production when 26 % O2 pulses were applied; nevertheless, conidiation in SSC was two orders of magnitude more sensitive to oxidant pulses. In SC with 26 % O2 pulses, conidia viability for both strains at early stages, was higher than in normal atmospheric conditions. Infectivity towards Galleria mellonella larvae was similar between conidia from normal atmosphere and oxidant conditions; notably, for the strain ARSEF 3302 infectivity decreased at the final stage. This study shows the intrinsic differences between SC and SSC, which should be considered when using SC as a model to design production processes in SSC.
Departamento de Biotecnología, Universidad Autónoma Metropolitana Iztapalapa, 09340, Iztapalapa, Mexico City, Mexico.
Facundo
F
Muñiz-Paredes
Instituto de Ciencias Agropecuarias, Universidad Autónoma del Estado de Hidalgo, 43600, Tulancingo, Hidalgo, Mexico.
Paul Misael
PM
Garza-López
Departamento de Biotecnología, Universidad Autónoma Metropolitana Iztapalapa, 09340, Iztapalapa, Mexico City, Mexico.
Gustavo
G
Viniegra-González
Departamento de Biotecnología, Universidad Autónoma Metropolitana Iztapalapa, 09340, Iztapalapa, Mexico City, Mexico. loera@xanum.uam.mx.
Octavio
O
Loera
D000818:Animals; D001274:Atmospheric Pressure; D003470:Culture Media; D006999:Hypocreales / Q000187:drug effects / Q000254:growth & development* / Q000472:pathogenicity / Q000502:physiology; D007814:Larva / Q000382:microbiology; D007915:Lepidoptera / Q000382:microbiology; D050296:Microbial Viability; D009172:Mycology / Q000379:methods; D016877:Oxidants / Q000494:pharmacology*; D010100:Oxygen / Q000494:pharmacology; D013172:Spores, Fungal / Q000254:growth & development / Q000502:physiology
D003470:Culture Media; D016877:Oxidants; D010100:Oxygen
Conidia production; Conidia quality; Oxidant sensitivity; Solid-state culture; Superficial culture