TRUE Gene Silencing: Screening of a Heptamer-type Small Guide RNA Library for Potential Cancer Therapeutic Agents.
TRUE gene silencing (termed after tRNase Z(L)-utilizing efficacious gene silencing) is one of the RNA-directed gene silencing technologies, which utilizes an artificial small guide RNA (sgRNA) to guide tRNA 3' processing endoribonuclease, tRNase Z(L), to recognize a target RNA. sgRNAs can be taken up by cells without any transfection reagents and can downregulate their target RNA levels and/or induce apoptosis in human cancer cells. We have screened an sgRNA library containing 156 heptamer-type sgRNAs for the effect on viability of human myeloma and leukemia cells, and found that 20 of them can efficiently induce apoptosis in at least one of the cancer cell lines. Here we present a protocol for screening of a heptamer-type sgRNA library for potential therapeutic drugs against blood cancers. The protocol includes how to construct the sgRNA library, how to assess the effect of each sgRNA on cell viability, and how to further evaluate the effective sgRNAs by flow cytometry. Around 2,000 hits would be expected to be obtained by screening the full-scale sgRNA library composed of 16,384 heptamers.
Research Institute for Healthy Living, Niigata University of Pharmacy and Applied Life Sciences.
Arisa
A
Haino
Research Institute for Healthy Living, Niigata University of Pharmacy and Applied Life Sciences.
Tatsuya
T
Ishikawa
Research Institute for Healthy Living, Niigata University of Pharmacy and Applied Life Sciences.
Mineaki
M
Seki
Research Institute for Healthy Living, Niigata University of Pharmacy and Applied Life Sciences; mnashimoto@nupals.ac.jp.
Masayuki
M
Nashimoto
D017209:Apoptosis; D015723:Gene Library; D020868:Gene Silencing; D006801:Humans; D009369:Neoplasms; D014162:Transfection; D000094704:RNA, Guide, CRISPR-Cas Systems
D000094704:RNA, Guide, CRISPR-Cas Systems