Antibiofilm activity of carboxymethyl chitosan on the biofilms of non-Candida albicans Candida species.
Although most cases of candidiasis have been attributed to Candida albicans, non-C. albicans Candida species have been isolated in increasing numbers in patients. In this study, we determined the inhibition of carboxymethyl chitosan (CM-chitosan) on single and mixed species biofilm of non-albicans Candida species, including Candida tropicalis, Candida parapsilosis, Candida krusei and Candida glabrata. Biofilm by all tested species in microtiter plates were inhibited nearly 70%. CM-chitosan inhibited mixed species biofilm in microtiter plates and also on medical materials surfaces. To investigate the mechanism, the effect of CM-chitosan on cell viability and biofilm growth was employed. CM-chitosan inhibited Candida planktonic growth as well as adhesion. Further biofilm formation was inhibited with CM-chitosan added at 90min, 12h or 24h after biofilm initiation. CM-chitosan was not only able to inhibit the metabolic activity of Candida cells, but was also active upon the establishment and the development of biofilms.
Department of Otorhinolaryngology and Head and Neck Surgery, Medical University of Vienna, Vienna, Austria. Electronic address: yulong.tan@meduniwien.ac.at.
Yulong
Y
Tan
Department of Otorhinolaryngology and Head and Neck Surgery, Medical University of Vienna, Vienna, Austria. Electronic address: matthias.leonhard@meduniwien.ac.at.
Matthias
M
Leonhard
Department of Cranio-Maxillofacial and Oral Surgery, Medical University of Vienna, Vienna, Austria. Electronic address: doris.moser@meduniwien.ac.at.
Doris
D
Moser
Department of Otorhinolaryngology and Head and Neck Surgery, Medical University of Vienna, Vienna, Austria. Electronic address: berit.schneider-stickler@meduniwien.ac.at.
Berit
B
Schneider-Stickler
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development*; D002175:Candida / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D002450:Cell Communication / Q000187:drug effects; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D012828:Silicones / Q000737:chemistry
D000900:Anti-Bacterial Agents; D012828:Silicones; C514968:carboxymethyl-chitosan; D048271:Chitosan
Biofilm; Carboxymethyl chitosan; Non-Candida albicans Candida species