Solvent-assisted stir bar sorptive extraction by using swollen polydimethylsiloxane for enhanced recovery of polar solutes in aqueous samples: Application to aroma compounds in beer and pesticides in wine.
A novel solvent-assisted stir bar sorptive extraction (SA-SBSE) technique was developed for enhanced recovery of polar solutes in aqueous samples. A conventional PDMS stir bar was swollen in several solvents with log Kow ranging from 1.0 to 3.5 while stirring for 30min prior to extraction. After extraction, thermal desorption - gas chromatography - (tandem) mass spectrometry (TD-GC-(MS/)MS) or liquid desorption - large volume injection (LD-LVI)-GC-MS were performed. An initial study involved investigation of potential solvents for SA-SBSE by weighing of the residual solvent in the swollen PDMS stir bar before and after extraction. Compared to conventional SBSE, SA-SBSE using diethyl ether, methyl isobutyl ketone, dichloromethane, diisopropyl ether and toluene provided higher recoveries from water samples for test solutes with log Kow<2.5. For SA-SBSE using dichloromethane, recoveries were improved by factors of 1.4-4.1, while maintaining or even improving the recoveries for test solutes with log Kow>2.5. The performance of the SA-SBSE method using dichloromethane, diisopropyl ether, and cyclohexane is illustrated with analyses of aroma compounds in beer and of pesticides in wine.
GERSTEL K.K. 1-3-1 Nakane, Meguro-ku, Tokyo 152-0031, Japan. Electronic address: nobuo_ochiai@gerstel.co.jp.
Nobuo
N
Ochiai
GERSTEL K.K. 1-3-1 Nakane, Meguro-ku, Tokyo 152-0031, Japan.
Kikuo
K
Sasamoto
Research Institute for Chromatography, President Kennedypark 26, 8500 Kortrijk, Belgium.
Frank
F
David
Research Institute for Chromatography, President Kennedypark 26, 8500 Kortrijk, Belgium.
Pat
P
Sandra
D000327:Adsorption; D001515:Beer / Q000032:analysis; D004129:Dimethylpolysiloxanes / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D008752:Methylene Chloride / Q000737:chemistry; D010575:Pesticides / Q000032:analysis*; D012997:Solvents / Q000737:chemistry*; D053719:Tandem Mass Spectrometry; D014867:Water / Q000737:chemistry*; D014920:Wine / Q000032:analysis*
D004129:Dimethylpolysiloxanes; D010575:Pesticides; D012997:Solvents; D014867:Water; D008752:Methylene Chloride; C013830:baysilon
Aroma compounds in beer; Pesticides in wine; Polar solutes; Solvent swollen PDMS; Solvent-assisted stir bar sorptive extraction (SA-SBSE)