Detection of herbaceous-plant pararetrovirus in lichen herbarium samples.
Cauliflower mosaic virus (CaMV) - a plant pararetrovirus that naturally causes diseases in Brassicaceae and Solanaceae plant hosts worldwide - has been detected by PCR for the first time in herbarium samples of Usnea sp. lichens. The virus's presence in these lichens did not result in any micro- or macromorphological changes, and the herbarium records were classified as representative for the distinct species. Sequence analyses classified all the detected viruses into one lineage of CaMV isolates. We have shown here that herbarium samples could be a good source for virus study, especially where a longer time span is involved.
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Petrzik
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Koloniuk
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Sarkisová
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Číhal
D017796:Caulimovirus / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000235:genetics; D008011:Lichens / Q000821:virology*; D016133:Polymerase Chain Reaction
D004279:DNA, Viral
cauliflower mosaic virus; dsDNA; pararetrovirus; persistent virus.