How to move an amphipathic molecule across a lipid bilayer: different mechanisms for different ABC transporters?
Import of β-oxidation substrates into peroxisomes is mediated by ATP binding cassette (ABC) transporters belonging to subfamily D. In order to enter the β-oxidation pathway, fatty acids are activated by conversion to fatty acyl-CoA esters, a reaction which is catalysed by acyl-CoA synthetases (ACSs). Here, we present evidence for an unusual transport mechanism, in which fatty acyl-CoA substrates are accepted by ABC subclass D protein (ABCD) transporters, cleaved by the transporters during transit across the lipid bilayer to release CoA, and ultimately re-esterified in the peroxisome lumen by ACSs which interact with the transporter. We propose that this solves the biophysical problem of moving an amphipathic molecule across the peroxisomal membrane, since the intrinsic thioesterase activity of the transporter permits separate membrane translocation pathways for the hydrophobic fatty acid moiety and the polar CoA moiety. The cleavage/re-esterification mechanism also has the potential to control entry of disparate substrates into the β-oxidation pathway when coupled with distinct peroxisomal ACSs. A different solution to the movement of amphipathic molecules across a lipid bilayer is deployed by the bacterial lipid-linked oligosaccharide (LLO) flippase, PglK, in which the hydrophilic head group and the hydrophobic polyprenyl tail of the substrate are proposed to have distinct translocation pathways but are not chemically separated during transport. We discuss a speculative alternating access model for ABCD proteins based on the mammalian ABC transporter associated with antigen processing (TAP) and compare it to the novel mechanism suggested by the recent PglK crystal structures and biochemical data.
Biological Chemistry and Crop Protection Department, Rothamsted Research, Harpenden, AL5 2JQ, U.K. freddie.theodoulou@rothamsted.ac.uk.
Frederica L
FL
Theodoulou
Centre for Plant Sciences, School of Molecular and Cellular Biology, University of Leeds, Leeds LS2 9JT, U.K.
David J
DJ
Carrier
Biological Chemistry and Crop Protection Department, Rothamsted Research, Harpenden, AL5 2JQ, U.K.
Theresia A
TA
Schaedler
Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, U.K.
Stephen A
SA
Baldwin
Centre for Plant Sciences, School of Molecular and Cellular Biology, University of Leeds, Leeds LS2 9JT, U.K.
Alison
A
Baker
D018528:ATP-Binding Cassette Transporters / Q000378:metabolism*; D000214:Acyl Coenzyme A / Q000378:metabolism*; D000818:Animals; D001419:Bacteria / Q000378:metabolism; D001692:Biological Transport; D003066:Coenzyme A Ligases / Q000378:metabolism*; D056890:Eukaryota / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D057927:Hydrophobic and Hydrophilic Interactions; D008051:Lipid Bilayers / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000378:metabolism; D020675:Peroxisomes / Q000378:metabolism*
D018528:ATP-Binding Cassette Transporters; D000214:Acyl Coenzyme A; D008051:Lipid Bilayers; D008070:Lipopolysaccharides; C023023:lipid-linked oligosaccharides; D003066:Coenzyme A Ligases
ABC transporter; acyl-CoA; asymmetry; mechanism; peroxisome; thioesterase; β-oxidation