Cloning of the cnr operon into a strain of Bacillaceae bacterium for the development of a suitable biosorbent.
In this study, a potential microbial biosorbent was engineered to improve its capacity to remediate heavy metal contaminated water resources. A Bacillaceae bacterium isolated from a mining area was transformed with a plasmid carrying the (pECD312)-based cnr operon that encodes nickel and cobalt resistance. The bioadsorption ability of the transformed strain was evaluated for removal of nickel from metallurgical water relative to the wildtype strain. Results showed that transformation improved the adsorption capacity of the bacterium by 37 % at nickel concentrations equivalent to 150 mg/L. Furthermore it was possible to apply prediction modelling to study the bioadsorption behaviour of the transformed strain. As such, this work may be extended to the design of a nickel bioremediation plant utilising the newly developed Bacillaceae bacterium as a biosorbent.
School of Chemical and Minerals Engineering, Faculty of Engineering, North-West University, Potchefstroom, South Africa. kaelpfr@yahoo.fr.
Elvis
E
Fosso-Kankeu
Minerals Processing and Technology Research Center, Department of Extraction Metallurgy, School of Mining, Metallurgy and Chemical Engineering, Faculty of Engineering and the Built Environment, University of Johannesburg, PO Box 17011, Doornfontein, 2028, South Africa.
Antoine F
AF
Mulaba-Bafubiandi
Department of Biochemistry, Faculty of Science, University of Johannesburg, PO Box 524, Auckland Park, 2006, South Africa.
Lizelle A
LA
Piater
Department of Biochemistry, Faculty of Science, University of Johannesburg, PO Box 524, Auckland Park, 2006, South Africa.
Matsobane G
MG
Tlou
D000327:Adsorption; D001406:Bacillaceae / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D001673:Biodegradation, Environmental; D018533:Biomass; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D019216:Metals, Heavy / Q000378:metabolism; D008906:Mining; D009876:Operon; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors; D014764:Viral Proteins; D014874:Water Pollutants, Chemical / Q000378:metabolism; D018508:Water Purification / Q000379:methods
C089636:Cnr protein, Bacteriophage P4; D019216:Metals, Heavy; D014157:Transcription Factors; D014764:Viral Proteins; D014874:Water Pollutants, Chemical
Adsorption behaviour; Bacillaceae bacterium; Bioremediation; Mining water; Nickel; Transformation