Pseudo-Ligandless Click Chemistry for Oligonucleotide Conjugation.
Particularly for its use in bioconjugations, the copper-catalyzed (or copper-promoted) azide-alkyne cycloaddition (CuAAC) reaction or 'click chemistry', has become an essential component of the modern chemical biologist's toolbox. Click chemistry has been applied to DNA, and more recently, RNA conjugations, and the protocols presented here can be used for either. The reaction can be carried out in aqueous buffer, and uses acetonitrile as a minor co-solvent that serves as a ligand to stabilize the copper. The method also includes details on the analysis of the reaction product. © 2016 by John Wiley & Sons, Inc.
Department of Chemistry and Center for Nucleic Acids Science & Technology, Carnegie Mellon University, Pittsburgh, Pennsylvania.|These authors contributed equally to this work.
Stephanie
S
Mack
Department of Chemistry and Center for Nucleic Acids Science & Technology, Carnegie Mellon University, Pittsburgh, Pennsylvania.|These authors contributed equally to this work.
Munira F
MF
Fouz
Department of Chemistry and Center for Nucleic Acids Science & Technology, Carnegie Mellon University, Pittsburgh, Pennsylvania.|These authors contributed equally to this work.
Sourav K
SK
Dey
Department of Chemistry and Center for Nucleic Acids Science & Technology, Carnegie Mellon University, Pittsburgh, Pennsylvania.
Subha R
SR
Das
D000480:Alkynes / Q000737:chemistry*; D001386:Azides / Q000737:chemistry*; D057930:Click Chemistry / Q000379:methods*; D004247:DNA / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry*; D012313:RNA / Q000737:chemistry
D000480:Alkynes; D001386:Azides; D009841:Oligonucleotides; D012313:RNA; D004247:DNA
DNA; RNA; alkyne; azide; bioconjugation; labeling; nucleic acids