Rationale behind the optimum efficiency of columns packed with new 1.9μm fully porous particles of narrow particle size distribution.
Columns packed with new commercially available 1.9 fully porous particles of narrow particle size distribution (nPSD) are characterized by extremely high efficiency. Under typical reversed phase conditions, these columns are able to generate very high number of theoretical plates (in the order of 300,000plates/m and more). In this paper, we investigate the origin of the high performance of these nPSD columns by performing a series of measurements that include, in addition to the traditional determination of the van Deemter curve, peak parking, pore blocking and inverse size exclusion experiments. Two nPSD columns (both 100×3.0mm) have been considered in this study: the first one, packed with particles of 80Å pore size, is commercially available. The second one is a prototype column packed with 1.9 fully porous particles of 120Å pore size. The main conclusion of our study is that these nPSD columns are characterized by extremely low eddy dispersion, while longitudinal diffusion and mass transfer kinetics are substantially equivalent to those of other fully porous particles of similar chemistry.
Dept. of Chemistry and Pharmaceutical Sciences, University of Ferrara, via L. Borsari 46, 44121 Ferrara, Italy.
Martina
M
Catani
Dept. of Drug Chemistry and Technology, "Sapienza" Università di Roma, P.le A. Moro 5, 00185 Roma, Italy.
Omar H
OH
Ismail
Dept. of Chemistry and Pharmaceutical Sciences, University of Ferrara, via L. Borsari 46, 44121 Ferrara, Italy. Electronic address: cvz@unife.it.
Alberto
A
Cavazzini
Dept. of Drug Chemistry and Technology, "Sapienza" Università di Roma, P.le A. Moro 5, 00185 Roma, Italy.
Alessia
A
Ciogli
Dept. of Drug Chemistry and Technology, "Sapienza" Università di Roma, P.le A. Moro 5, 00185 Roma, Italy.
Claudio
C
Villani
Dept. of Chemistry and Pharmaceutical Sciences, University of Ferrara, via L. Borsari 46, 44121 Ferrara, Italy.
Luisa
L
Pasti
Dept. of Chemistry and Pharmaceutical Sciences, University of Ferrara, via L. Borsari 46, 44121 Ferrara, Italy.
Caterina
C
Bergantin
KU Leuven, Department of Pharmaceutical Sciences, Pharmaceutical Analysis, Herestraat 49, Leuven 3000, Belgium.
Deirdre
D
Cabooter
Vrije Universiteit Brussel (VUB), Department of Chemical Engineering, 1050 Brussels, Belgium.
Gert
G
Desmet
Dept. of Drug Chemistry and Technology, "Sapienza" Università di Roma, P.le A. Moro 5, 00185 Roma, Italy.
Francesco
F
Gasparrini
Applied Research and Development, Millipore Sigma, 595 North Harrison Road, Bellefonte, PA 16823, USA.
David S
DS
Bell
D056148:Chromatography, Reverse-Phase / Q000295:instrumentation*; D008962:Models, Theoretical; D010316:Particle Size; D016062:Porosity
Column efficiency; Eddy dispersion; Mass transfer; Sub-2μm fully porous particles of narrow particle size distribution (nPSD)