Antimicrobial application of nanofibrous mats self-assembled with chitosan and epigallocatechin gallate.
Cellulose electrospun nanofibrous mats coated with bilayers of chitosan (CS) and epigallocatechin gallate (EGCG) or with bilayers of CS-rectorite (REC) composite (CS-REC) and EGCG were fabricated via layer-by-layer (LBL) self-assembly technique. LBL-structured cellulose nanofibers still maintained three-dimension fiber structure according to the observation from scanning electron microscopy images. The average diameter of nanofibers were enlarged with the addition of REC. X-ray photoelectron spectroscopy results confirmed the deposition of CS and CS-REC onto the corresponding mats. The tensile strength and rate of elongation at break of LBL-structured nanofibers had no difference from those of uncoated nanofibers. The encapsulation efficiency and loading capacity of nanofibers were enhanced in the presence of REC. In addition the in-vitro cumulative release profiles of EGCG indicate that the addition of REC delayed the release of EGCG. Antimicrobial assay demonstrated the inhibitory effects of CS and EGCG on the growth of Staphylococcus aureus. Meanwhile the CS-REC composites improved the antimicrobial effects of CS and EGCG by adsorption of bacteria to the surface of REC then enhancement of exposure of bacteria to EGCG and the matrix of CS.
College of Food Science and Technology and MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, China.
Jing
J
Tian
Hubei International Scientific and Technological Cooperation Base of Sustainable Resource and Energy, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China.
Hu
H
Tu
Hubei International Scientific and Technological Cooperation Base of Sustainable Resource and Energy, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China.
Xiaowen
X
Shi
State Key Laboratory of Pulp and Paper Engineering, School of Light Industry and Food, South China University of Technology, Guangzhou 510640, China.
Xiaoying
X
Wang
Hubei International Scientific and Technological Cooperation Base of Sustainable Resource and Energy, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China. Electronic address: hbdeng@whu.edu.cn.
Hongbing
H
Deng
College of Food Science and Technology and MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: libinfood@mail.hzau.edu.cn.
Bin
B
Li
Hubei International Scientific and Technological Cooperation Base of Sustainable Resource and Energy, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China.
Yumin
Y
Du
D000538:Aluminum Silicates / Q000737:chemistry; D000890:Anti-Infective Agents / Q000494:pharmacology*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002482:Cellulose / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D008903:Minerals / Q000737:chemistry; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D056951:Photoelectron Spectroscopy; D013211:Staphylococcus aureus / Q000187:drug effects; D055672:Static Electricity; D013718:Tensile Strength
D000538:Aluminum Silicates; D000890:Anti-Infective Agents; D008903:Minerals; C546376:rectorite; D002392:Catechin; D002482:Cellulose; D048271:Chitosan; C045651:epigallocatechin gallate
Antimicrobial activity; Cellulose nanofibrous mats; Chitosan; Epigallocatechin gallate; Layer-by-layer; Rectorite