Life Span and Motility Effects of Ethanolic Extracts from Sophora moorcroftiana Seeds on Caenorhabditis elegans.
BACKGROUND
Sophora moorcroftiana is an endemic shrub species with a great value in folk medicine in Tibet, China. In this study, relatively little is known about whether S. moorcroftiana is beneficial in animals' nervous system and life span or not.


METHODS
To address this question, under survival normal temperature (25°C), S. moorcroftiana seeds were extracted with 95% ethanol, and Caenorhabditis elegans were exposed to three different extract concentrations (100 mg/L, 200 mg/L, and 400 mg/mL) from S. moorcroftiana seeds.


RESULTS
The 95% ethanolic extracts from S. moorcroftiana seeds could increase life span and slow aging-related increase in C. elegans and could not obviously influence the motility of C. elegans.


CONCLUSIONS
Given these results by our experiment for life span and motility with 95% ethanolic extracts from S. moorcroftiana seeds in C. elegans, the question whether S. moorcroftiana acts as an anti-aging substance in vivo arises.


CONCLUSIONS
The 95% ethanolic extracts from S. moorcroftiana seeds have no effect on the life span in C. elegans when extract concentrations from S. moorcroftiana seeds <400 mg/LThe 400 mg/L 95% ethanolic extracts from S. moorcroftiana seeds could increase life span in C. elegansThe 95% ethanolic extracts from S. moorcroftiana seeds could not obviously influence the motility in C. elegans. Abbreviation used: S. moorcroftiana: Sophora moorcroftiana; C. elegan: Caenorhabditis elegan; E. coli OP50: Escherichia coli OP50; DMSO: Dimethyl sulfoxide.
Department of Immunology, School of Basic Medical Sciences, Lanzhou 730000, China.
Xin
X
Li
Department of Immunology, School of Basic Medical Sciences, Lanzhou 730000, China.
Junxian
J
Han
Department of Immunology, School of Basic Medical Sciences, Lanzhou 730000, China.
Rongyan
R
Zhu
Department of Immunology, School of Basic Medical Sciences, Lanzhou 730000, China.
Rongrong
R
Cui
Department of Immunology, School of Basic Medical Sciences, Lanzhou 730000, China; Key Laboratory of Evidence Based Medicine and Knowledge Translation of Gansu Province, Lanzhou University, Lanzhou 730000, China.
Xingming
X
Ma
Department of Microbiology, Medical College, Northwest University for Nationalities, Lanzhou 730000, China.
Kaizhong
K
Dong
Anti-aging; Caenorhabditis elegans; Sophora moorcroftiana; motility