A Hexa-Herbal TCM Decoction Used to Treat Skin Inflammation: An LC-MS-Based Phytochemical Analysis.
In order to understand the chemical relationship between a traditional hexa-herbal Chinese medicine formula and botanical drugs it is derived from, an analytical platform comprising liquid chromatography coupled with triple quadrupole mass spectrometry and data mining was developed to separate and identify key chemical components. The hexa-herbal formula comprises the rootstock of Scutellaria baicalensis, Rheum tanguticum, Sophora flavescens, the root bark of Dictamnus dasycarpus, the bark of Phellodendron chinense, and the fruit of Kochia scoparia. Seventy-three compounds including alkaloids, anthraquinone derivatives, coumarins, coumarins derivatives, flavonoids, flavone glycosides, naphthalene derivatives, phenylbutanone glucopyranoside, phenolic acids, pterocarpans, stilbenes, stilbenes derivatives, and tannins were putatively identified based on mass measurement and characteristic fragment ions. Among the botanical drugs of the hexa-herbal Chinese medicine formula, the rootstock of R. tanguticum and S. flavescens, bark of P. chinense, and rootstock of S. baicalensis contributed to the majority of the extracted metabolites of the formula decoction. The developed method appeared to be a versatile tool for monitoring chemical constituents in extracts of a traditional Chinese medicine formula in a relatively comprehensive and systematic manner, and helped to understand the importance of the individual botanical drugs within a formulation.
Research Cluster Biodiversity and Medicines/Centre for Pharmacognosy and Phytotherapy, UCL School of Pharmacy, London, U. K.
Jennifer B
JB
Chang
Department of Pharmaceutics, UCL School of Pharmacy, London, U. K.
Majella E
ME
Lane
Department of Pharmaceutical & Biological Chemistry, UCL School of Pharmacy, London, U. K.
Min
M
Yang
Research Cluster Biodiversity and Medicines/Centre for Pharmacognosy and Phytotherapy, UCL School of Pharmacy, London, U. K.
Michael
M
Heinrich
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000302:isolation & purification* / Q000627:therapeutic use; D002853:Chromatography, Liquid; D003872:Dermatitis / Q000188:drug therapy*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000627:therapeutic use; D015394:Molecular Structure; D064209:Phytochemicals / Q000032:analysis / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D053719:Tandem Mass Spectrometry; D000069459:Teas, Herbal
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004365:Drugs, Chinese Herbal; D064209:Phytochemicals; D000069459:Teas, Herbal