Effects of transferrin conjugated multi-walled carbon nanotubes in lung cancer delivery.
The aim of this study was to develop multi-walled carbon nanotubes (MWCNT) which were covalently conjugated with transferrin by carbodiimide chemistry and loaded with docetaxel as a model drug for effective treatment of lung cancer in comparison with the commercial docetaxel injection (Docel™). d-Alpha-tocopheryl polyethylene glycol 1000 succinate (TPGS) was used as amphiphilic surfactant to improve the aqueous dispersity and biocompatibility of MWCNT. Human lung cancer cells (A549 cells) were employed as an in-vitro model to access cellular uptake, cytotoxicity, cellular apoptosis, cell cycle analysis, and reactive oxygen species (ROS) of the docetaxel/coumarin-6 loaded MWCNT. The cellular uptake results of transferrin conjugated MWCNT showed higher efficiency in comparison with free C6. The IC50 values demonstrated that the transferrin conjugated MWCNT could be 136-fold more efficient than Docel™ after 24h treatment with the A549 cells. Flow cytometry analysis confirmed that cancerous cells appeared significantly (P<0.05) in the sub-G1 phase for transferrin conjugated MWCNT in comparison with Docel™. Results of transferrin conjugated MWCNT have showed better efficacy with safety than Docel™.
Department of Pharmacology, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India.
Rahul Pratap
RP
Singh
Genotoxicology and Cancer Biology Lab, Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi 221005, India.
Gunjan
G
Sharma
Department of Pharmacology, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India.
Sonali
Department of Pharmaceutics, Indian Institute of Technology (BHU), Varanasi 221005, India.
Sanjay
S
Singh
Department of Pathology, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India.
Shashikant C U
SCU
Patne
Department of Pharmacology, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India.
Bajarangprasad L
BL
Pandey
Genotoxicology and Cancer Biology Lab, Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi 221005, India. Electronic address: kochbiplob@gmail.com.
Biplob
B
Koch
Department of Pharmaceutics, Indian Institute of Technology (BHU), Varanasi 221005, India; Department of Pharmacology, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India. Electronic address: muthubits@rediffmail.com.
Madaswamy S
MS
Muthu
D000072283:A549 Cells; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001992:Bronchoalveolar Lavage Fluid; D002453:Cell Cycle / Q000187:drug effects; D003374:Coumarins / Q000737:chemistry; D000077143:Docetaxel; D016503:Drug Delivery Systems / Q000379:methods*; D065546:Drug Liberation; D004705:Endocytosis / Q000187:drug effects; D005434:Flow Cytometry; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D008168:Lung / Q000187:drug effects / Q000473:pathology; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000473:pathology; D018625:Microscopy, Atomic Force; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D013194:Staining and Labeling; D055672:Static Electricity; D043823:Taxoids / Q000494:pharmacology / Q000627:therapeutic use; D013844:Thiazoles / Q000737:chemistry; D014168:Transferrin / Q000378:metabolism*
D003374:Coumarins; D037742:Nanotubes, Carbon; D017382:Reactive Oxygen Species; D043823:Taxoids; D013844:Thiazoles; D014168:Transferrin; C517282:coumarin 6; D000077143:Docetaxel
Docetaxel; Endocytosis; Lung cancer; Nanomedicine; Safety; Transferrin conjugation