Halorussus salinus sp. nov., isolated from a marine solar saltern.
A halophilic archaeal strain YJ-37-HTwas isolated from Yangjiang marine solar saltern, China. Cells were pleomorphic rods, stained Gram negative and formed red-pigmented colonies on agar plate. Strain YJ-37-HTwas able to grow at 20-50 °C (optimum 37 °C), at 0.9-4.8 M NaCl (optimum 2.6 M NaCl), at 0-1.0 M MgCl2(optimum 0.3 MgCl2) and at pH 6.5-9.0 (optimum pH 7.0). The cells lysed in distilled water, and the minimal NaCl concentration to prevent cell lysis was found to be 5 % (w/v). The 16S rRNA gene and rpoB' gene of strain YJ-37-HTwere phylogenetically related to the corresponding genes of Halorussus members (93.2-95.8 % and 90.1-93.9 % similarities, respectively). The major polar lipids of the strain were phosphatidic acid (PA), phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), phosphatidylglycerol sulfate (PGS) and five glycolipids, sulfated galactosyl mannosyl glucosyl diether (S-TGD-1), galactosyl mannosyl glucosyl diether (TGD-1), sulfated mannosyl glucosyl diether (S-DGD-1), mannosyl glucosyl diether (DGD-1) and diglycosyl diether (DGD-2). The DNA G+C content of strain YJ-37-HTwas 64.9 mol%. The phenotypic, chemotaxonomic and phylogenetic properties suggested that strain YJ-37-HT(=CGMCC 1.12571T= JCM 30032T) represents a new species of Halorussus, for which the name Halorussus salinus sp. nov. is proposed.
School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People's Republic of China.
Jia-Qi
JQ
Xu
School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People's Republic of China.
Wen-Mei
WM
Xu
School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People's Republic of China.
Yang
Y
Li
School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People's Republic of China.
Yao
Y
Zhou
School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People's Republic of China.
Zhen-Zhen
ZZ
Lü
School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People's Republic of China.
Jing
J
Hou
School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People's Republic of China.
Lin
L
Zhu
School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People's Republic of China. cuihenglin@ujs.edu.cn.
Heng-Lin
HL
Cui
D001482:Base Composition; D002681:China; D019641:DNA, Archaeal / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D006017:Glycolipids / Q000032:analysis; D006216:Halobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012965:Sodium Chloride / Q000032:analysis
D019641:DNA, Archaeal; D006017:Glycolipids; C407124:PGP-Me; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; C407123:S-TGD-1; D012965:Sodium Chloride; D012321:DNA-Directed RNA Polymerases
Halophilic archaeon; Halorussus salinus sp. nov.; Marine solar saltern