Pharmacokinetic comparative study of gastrodin after oral administration of Gastrodia elata Bl. extract and its compatibility with the different indigents of Ligusticum chuanxiong Hort. to rats.
BACKGROUND
Da Chuan Xiong Decoction Compound preparation (DCXDCP) is a classic TCM formula of an aqueous extract made from Chuanxiong Rhizoma (Ligusticum chuanxiong Hort., umbelliferae) and Tianma Rhizoma (Gastrodia elata Bl., Orchidaceae). Gastrodin (GAS), a bioactive component of tianma, its pharmacokinetic (PK) behavior significantly changed after oral administration of DCXDCP compared with the extract of tianma. However, little is known about how the ingredients of chuanxiong influenced on the PK of GAS.


OBJECTIVE
To study the possible PK behavior differences of GAS after individually oral administration of tianma extract and tianma extract mixed with different active ingredients of chuanxiong to rats, as well as explore whether there were some herb-herb interactions.


METHODS
Different DCXDCP suspensions were prepared by mixing tianma extract with different active ingredients of chuanxiong. The rats were randomly assigned to six groups and were orally treated with different DCXDCP. At different predetermined time points after administration, the concentrations of GAS in the rat plasma were determined using HPLC, and the main PK parameters were investigated.


RESULTS
The results showed that tetramethylpyrazine had no significant effects on the PK parameters of GAS (p>0.05), whereas ferulic acid (FA), total phenolic acids and total alkaloids significantly increased AUC0-∞ (p<0.05). In general the observed changes in the PK parameters of GAS in DCXDCP could be closely related to the total phenolic acids and total alkaloids.


CONCLUSIONS
It could be shown that total phenolic acids and total alkaloids present in Ligusticum chuanxiong in addition to other components not tested yet play an important role in affecting the PK of gastrodin in DCXDCP.
Key Lab of Modern Preparation of Traditional Chinese Medicine, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
Peng-Yi
PY
Hu
Key Lab of Modern Preparation of Traditional Chinese Medicine, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
Peng-Fei
PF
Yue
Key Lab of Modern Preparation of Traditional Chinese Medicine, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China. Electronic address: zhengqin912006@163.com.
Qin
Q
Zheng
Key Lab of Modern Preparation of Traditional Chinese Medicine, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
Ming
M
Yang
Key Lab of Modern Preparation of Traditional Chinese Medicine, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
Guo-Song
GS
Zhang
Key Lab of Modern Preparation of Traditional Chinese Medicine, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
Bin
B
Wu
Key Lab of Modern Preparation of Traditional Chinese Medicine, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
Dan
D
Liu
D000284:Administration, Oral; D000470:Alkaloids / Q000008:administration & dosage / Q000493:pharmacokinetics; D000818:Animals; D019540:Area Under Curve; D001592:Benzyl Alcohols / Q000008:administration & dosage* / Q000097:blood / Q000302:isolation & purification / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000008:administration & dosage / Q000493:pharmacokinetics; D004347:Drug Interactions; D005260:Female; D031668:Gastrodia / Q000737:chemistry*; D005960:Glucosides / Q000008:administration & dosage* / Q000097:blood / Q000302:isolation & purification / Q000493:pharmacokinetics*; D006207:Half-Life; D062385:Hydroxybenzoates / Q000008:administration & dosage / Q000493:pharmacokinetics; D030004:Ligusticum / Q000737:chemistry*; D008657:Metabolic Clearance Rate; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage* / Q000097:blood / Q000302:isolation & purification / Q000493:pharmacokinetics*; D010946:Plants, Medicinal; D011719:Pyrazines / Q000008:administration & dosage / Q000493:pharmacokinetics; D017208:Rats, Wistar
D000470:Alkaloids; D001592:Benzyl Alcohols; D003373:Coumaric Acids; D005960:Glucosides; D062385:Hydroxybenzoates; D010936:Plant Extracts; D011719:Pyrazines; C045345:gastrodin; C004999:ferulic acid; C017616:phenolic acid; C017953:tetramethylpyrazine
Chuanxiong; Drug-drug interaction; Ferulic acid; Gastrodin; Pharmacokinetics; Tetramethylpyrazine; Tianma; Total alkaloids; Total phenolic acid