Rapid and efficient degradation of bisphenol A by chloroperoxidase from Caldariomyces fumago: product analysis and ecotoxicity evaluation of the degraded solution.
OBJECTIVE
To degrade enzymatically bisphenol A (BPA) that causes serious environmental concerns and is difficult to be degraded by chemical or physical methods.


RESULTS
BPA (150 mg l(-1)) was completely degraded by chloroperoxidase (CPO)/H2O2 within 7 min at room temperature, atmospheric pressure with the enzyme at 6 μg CPO ml(-1). The degradation products were identified by HPLC-MS, which suggested involvement of multiple steps. Enzymatic treatment followed by existing bioremediation technologies (activated sludge) enhanced removal of COD from 9 to 54 %. Using an ecotoxicity evaluation with Chlorella pyrenoidosa, the degradation products had a lower toxicity than BPA.


CONCLUSIONS
BPA can be degraded rapidly and efficiently under mild conditions with chloroperoxidase at 6 μg ml(-1). The degradation products had a lower toxicity than BPA.
School of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an, 710062, People's Republic of China.
Xiaobo
X
Dong
School of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an, 710062, People's Republic of China.
Haiyun
H
Li
School of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an, 710062, People's Republic of China. jyc@snnu.edu.cn.|Key Laboratory of Macromolecular Science of Shaanxi Province, Shaanxi Normal University, Xi'an, 710062, People's Republic of China. jyc@snnu.edu.cn.|, No. 620 West Chang'an Road, Chang'an District, Xi'an, 710119, People's Republic of China. jyc@snnu.edu.cn.
Yucheng
Y
Jiang
School of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an, 710062, People's Republic of China.|Key Laboratory of Macromolecular Science of Shaanxi Province, Shaanxi Normal University, Xi'an, 710062, People's Republic of China.
Mancheng
M
Hu
School of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an, 710062, People's Republic of China.|Key Laboratory of Macromolecular Science of Shaanxi Province, Shaanxi Normal University, Xi'an, 710062, People's Republic of China.
Shuni
S
Li
School of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an, 710062, People's Republic of China.|Key Laboratory of Macromolecular Science of Shaanxi Province, Shaanxi Normal University, Xi'an, 710062, People's Republic of China.
Quanguo
Q
Zhai
D001559:Benzhydryl Compounds / Q000378:metabolism*; D002708:Chlorella / Q000378:metabolism; D002711:Chloride Peroxidase / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D010636:Phenols / Q000378:metabolism*; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D002711:Chloride Peroxidase; D006861:Hydrogen Peroxide; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds
Bisphenol A; Chloroperoxidase; Ecotoxicity evaluation; Enzymatic degradation