In vitro mesenchymal stem cell response to a CO2 laser modified polymeric material.
With an ageing world population it is becoming significantly apparent that there is a need to produce implants and platforms to manipulate stem cell growth on a pharmaceutical scale. This is needed to meet the socio-economic demands of many countries worldwide. This paper details one of the first ever studies in to the manipulation of stem cell growth on CO2 laser surface treated nylon 6,6 highlighting its potential as an inexpensive platform to manipulate stem cell growth on a pharmaceutical scale. Through CO2 laser surface treatment discrete changes to the surfaces were made. That is, the surface roughness of the nylon 6,6 was increased by up to 4.3μm, the contact angle was modulated by up to 5° and the surface oxygen content increased by up to 1atom %. Following mesenchymal stem cell growth on the laser treated samples, it was identified that CO2 laser surface treatment gave rise to an enhanced response with an increase in viable cell count of up to 60,000cells/ml when compared to the as-received sample. The effect of surface parameters modified by the CO2 laser surface treatment on the mesenchymal stem cell response is also discussed along with potential trends that could be identified to govern the mesenchymal stem cell response.
Laser Engineering and Manufacturing Research Centre, Faculty of Science and Engineering, University of Chester, Chester CH1 4BJ, UK. Electronic address: d.waugh@chester.ac.uk.
D G
DG
Waugh
School of Life Sciences, Brayford Pool, University of Lincoln, Lincoln LN6 7TS, UK.
I
I
Hussain
Laser Engineering and Manufacturing Research Centre, Faculty of Science and Engineering, University of Chester, Chester CH1 4BJ, UK.
J
J
Lawrence
Laser Engineering and Manufacturing Research Centre, Faculty of Science and Engineering, University of Chester, Chester CH1 4BJ, UK.
G C
GC
Smith
School of Life Sciences, Brayford Pool, University of Lincoln, Lincoln LN6 7TS, UK.
D
D
Cosgrove
Laser Engineering and Manufacturing Research Centre, Faculty of Science and Engineering, University of Chester, Chester CH1 4BJ, UK.
C
C
Toccaceli
D002209:Caprolactam / Q000031:analogs & derivatives* / Q000737:chemistry; D002478:Cells, Cultured; D006801:Humans; D054020:Lasers, Gas; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry*; D013499:Surface Properties
D011108:Polymers; C009916:nylon 6; D002209:Caprolactam
Adhesion; CO(2) laser; Contact angle; Stem cells; Surface engineering; Wettability