Crocetin ester improves myocardial ischemia via Rho/ROCK/NF-κB pathway.
Crocetin ester (CE) is the active ingredient of Crocus sativus L. stigmas and Gardenia jasminoides Ellis fruit. The main purpose of the present study was to investigate the protective effect of CE on isoproterenol (ISO)-induced acute myocardial ischemia (AMI) through Rho/ROCK/NF-κB pathway and explore its underlying mechanism. Administration of CE (25 and 50mg/kg) could significantly reduce the serum contents of pro-inflammatory cytokines including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β) and interleukin-6 (IL-6). In addition, pretreatment with CE attenuated the contents of creatine kinase (CK), malondialdehyde (MDA) and the activities of lactate dehydrogenase (LDH), superoxide dismutase (SOD) in serum. Treatment with CE also improved the histopathological alteration and decreased the ST elevation. Furthermore, CE could ameliorate the cardiac expressions of Cu, Zn-superoxide dismutase (SOD1), MDA5, Rho, ROCK, p-IκB and p-NF-κBp65 in ISO-induced rats. It was assumed that CE might be a new therapeutic candidate for the treatment of AMI possibly through the inhibition of Rho/ROCK/NF-κB pathway.
Center for Translational Systems Biology and Neuroscience, School of Basic Biomedical Science, Nanjing University of Chinese Medicine, Nanjing 210023, China.
Zhiheng
Z
Huang
Medical College of Soochow University, Suzhou 215000, China.
Chen
C
Nan
College of Pharmacy, Ningxia Medical University, Ningxia 750004, China.
Hanqing
H
Wang
Center for Translational Systems Biology and Neuroscience, School of Basic Biomedical Science, Nanjing University of Chinese Medicine, Nanjing 210023, China.
Qiang
Q
Su
Center for Translational Systems Biology and Neuroscience, School of Basic Biomedical Science, Nanjing University of Chinese Medicine, Nanjing 210023, China.
Wenda
W
Xue
Center for Translational Systems Biology and Neuroscience, School of Basic Biomedical Science, Nanjing University of Chinese Medicine, Nanjing 210023, China.
Yanyan
Y
Chen
Center for Translational Systems Biology and Neuroscience, School of Basic Biomedical Science, Nanjing University of Chinese Medicine, Nanjing 210023, China.
Xin
X
Shan
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China.
Jinao
J
Duan
Center for Translational Systems Biology and Neuroscience, School of Basic Biomedical Science, Nanjing University of Chinese Medicine, Nanjing 210023, China.
Gang
G
Chen
Center for Translational Systems Biology and Neuroscience, School of Basic Biomedical Science, Nanjing University of Chinese Medicine, Nanjing 210023, China; Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. Electronic address: taoweiweinanjing@126.com.
Weiwei
W
Tao
D000818:Animals; D002338:Carotenoids / Q000627:therapeutic use*; D003402:Creatine Kinase / Q000378:metabolism; D027622:Crocus / Q000276:immunology*; D016207:Cytokines / Q000378:metabolism; D006321:Heart / Q000187:drug effects*; D018836:Inflammation Mediators / Q000378:metabolism; D017202:Myocardial Ischemia / Q000139:chemically induced / Q000188:drug therapy*; D009206:Myocardium / Q000473:pathology*; D016328:NF-kappa B / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects; D000072105:Superoxide Dismutase-1 / Q000378:metabolism; D014801:Vitamin A / Q000031:analogs & derivatives; D054460:rho-Associated Kinases / Q000378:metabolism
D016207:Cytokines; D018836:Inflammation Mediators; D016328:NF-kappa B; C487773:trans-sodium crocetinate; D014801:Vitamin A; D002338:Carotenoids; C000606292:Sod1 protein, rat; D000072105:Superoxide Dismutase-1; D054460:rho-Associated Kinases; D003402:Creatine Kinase
Crocetin ester; Myocardial ischemia; NF-κB; Rho