Temporal alterations in the bovine buttermilk glycome from parturition to milk maturation.
The bovine milk fat globule membrane (MFGM) has many associated biological activities, many of which are linked with specific carbohydrate structures of MFGM glycoconjugates. Bovine buttermilk is a commercially viable source of MFGM and is an under-valued by-product of butter making. However, the changes in buttermilk glycosylation over the course of lactation have not been extensively investigated. In this study, buttermilk was generated from three individual multiparous cows at 13 time points over the first three months of lactation. Buttermilk glycosylation was profiled using lectin microarrays and lectin blotting. Suggested differences in glycosylation, including N-glycosylation, sialylation and fucosylation, were observed between early and late time points and between individual animals. Overall, these data suggest temporal changes in the glycosylation of buttermilk proteins which may have an important impact on commercial isolation of glycosylated ingredients.
Teagasc Food Research Centre, Moorepark, Fermoy, Co. Cork, Ireland; Glycoscience Group, National Centre for Biomedical Engineering Science, National University of Ireland Galway, Galway, Ireland.
Sarah A
SA
Ross
Glycoscience Group, National Centre for Biomedical Engineering Science, National University of Ireland Galway, Galway, Ireland.
Jared Q
JQ
Gerlach
Glycoscience Group, National Centre for Biomedical Engineering Science, National University of Ireland Galway, Galway, Ireland.
Satbir K
SK
Gill
Teagasc Food Research Centre, Moorepark, Fermoy, Co. Cork, Ireland.
Jonathan A
JA
Lane
Glycoscience Group, National Centre for Biomedical Engineering Science, National University of Ireland Galway, Galway, Ireland; Carbohydrate Signalling Group, Microbiology, School of Natural Sciences, National University of Ireland Galway, Galway, Ireland.
Michelle
M
Kilcoyne
Teagasc Food Research Centre, Moorepark, Fermoy, Co. Cork, Ireland.
Rita M
RM
Hickey
Glycoscience Group, National Centre for Biomedical Engineering Science, National University of Ireland Galway, Galway, Ireland. Electronic address: lokesh.joshi@nuigalway.ie.
Lokesh
L
Joshi
D000818:Animals; D000070696:Buttermilk / Q000032:analysis*; D002417:Cattle; D043302:Cultured Milk Products / Q000032:analysis; D005260:Female; D006017:Glycolipids / Q000032:analysis* / Q000378:metabolism*; D006023:Glycoproteins / Q000032:analysis* / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D007774:Lactation / Q000378:metabolism; D037102:Lectins / Q000032:analysis / Q000378:metabolism; D066292:Lipid Droplets; D008892:Milk / Q000737:chemistry; D036801:Parturition / Q000378:metabolism*; D011485:Protein Binding / Q000502:physiology; D013997:Time Factors
D006017:Glycolipids; D006023:Glycoproteins; D037102:Lectins; C110367:milk fat globule
Buttermilk; Glycoconjugates; Glycosylation; Lectin microarray; Milk fat globule membrane