Neutral endopeptidase and natriuretic peptide receptors participate in the regulation of C-type natriuretic peptide expression in renal interstitial fibrosis.
The initiation and progression of renal interstitial fibrosis (RIF) is a complicated process in which many factors may play an activate role. Among these factors, C-type natriuretic peptide (CNP) is an endothelium-derived hormone and acts in a local, paracrine fashion to regulate vascular smooth muscle tone and proliferation. In this study, we established a rat model of unilateral ureteral obstruction (UUO). CNP expression tends to be higher immediately after ligation and declined at later time points, occurring predominantly in tubular epithelial cells. A high-level CNP may contribute to the elevated expression of natriuretic peptide receptor (NPR)-B in the early phase of UUO. However, the sustained expression of NPR-C and neutral endopeptidase (NEP) observed throughout the study period (that is up to 3 months) helps to, at least partly, explain the subsequent decline of CNP. Thus, NEP and NPRs participate in the regulation of CNP expression in RIF.
a Department of Pediatrics , the First Affiliated Hospital of Anhui Medical University , Hefei , People's Republic of China.
Peng
P
Hu
a Department of Pediatrics , the First Affiliated Hospital of Anhui Medical University , Hefei , People's Republic of China.
Xun
X
Xia
b Department of Urology , Anhui Provincial Hospital, Anhui Medical University , Hefei , People's Republic of China.
Qiang
Q
Xuan
a Department of Pediatrics , the First Affiliated Hospital of Anhui Medical University , Hefei , People's Republic of China.
Bao Yu
BY
Huang
a Department of Pediatrics , the First Affiliated Hospital of Anhui Medical University , Hefei , People's Republic of China.
Si Yan
SY
Liu
a Department of Pediatrics , the First Affiliated Hospital of Anhui Medical University , Hefei , People's Republic of China.
Dong Dong
DD
Zhang
a Department of Pediatrics , the First Affiliated Hospital of Anhui Medical University , Hefei , People's Republic of China.
Guang Mei
GM
Jiang
a Department of Pediatrics , the First Affiliated Hospital of Anhui Medical University , Hefei , People's Republic of China.
Yao
Y
Xu
c Department of Pediatrics , First Affiliated Hospital of Guangxi Medical University , Nanning , People's Republic of China.
Yuan Han
YH
Qin
D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D005355:Fibrosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D018507:Gene Expression Regulation, Developmental; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D007674:Kidney Diseases / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008297:Male; D020098:Natriuretic Peptide, C-Type / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D017461:Receptors, Atrial Natriuretic Factor / Q000235:genetics / Q000378:metabolism*; D000099242:Atrial Natriuretic Factor Receptor B
D010450:Endopeptidases; D020098:Natriuretic Peptide, C-Type; D017461:Receptors, Atrial Natriuretic Factor; D000099242:Atrial Natriuretic Factor Receptor B
C-type natriuretic peptide; natriuretic peptide receptor; neutral endopeptidase; renal interstitial fibrosis; unilateral ureteral obstruction