Direct imaging of elemental distributions in tissue sections by laser ablation mass spectrometry.
We present a strategy for imaging of elements in biological tissues using laser ablation (LA) mass spectrometry (MS), which was compared to laser ablation inductively coupled plasma (LA-ICP) MS. Both methods were adopted for quantitative imaging of elements in mouse kidney, as well as traumatic brain injury model tissue sections. MS imaging (MSI) employing LA provides quantitative data by comparing signal abundances of sodium from tissues to those obtained by imaging quantitation calibration standards of the target element applied to adjacent control tissue sections. LA-ICP MSI provided quantitative data for several essential elements in both brain and kidney tissue sections using a dried-droplet approach. Both methods were used to image a rat model of traumatic brain injury, revealing accumulations of sodium and calcium in the impact area and its peripheral regions. LA MSI is shown to be a viable option for quantitative imaging of specific elements in biological tissue sections.
Biomolecular Imaging and Proteomics, Dept. of Pharmaceutical Biosciences, Uppsala University, Box 591 BMC, SE-75124 Uppsala, Sweden.
Mohammadreza
M
Shariatgorji
Biomolecular Imaging and Proteomics, Dept. of Pharmaceutical Biosciences, Uppsala University, Box 591 BMC, SE-75124 Uppsala, Sweden.
Anna
A
Nilsson
TU Wien, Institute of Chemical Technologies and Analytics, Vienna, Austria.
Maximilian
M
Bonta
Biomolecular Imaging and Proteomics, Dept. of Pharmaceutical Biosciences, Uppsala University, Box 591 BMC, SE-75124 Uppsala, Sweden; Department of Chemistry, Institute of Biomedical Sciences and State Key Lab of Molecular Engineering of Polymers, Fudan University, Shanghai, China.
Jinrui
J
Gan
Department of Neuroscience, Neurosurgery, Uppsala University Hospital, Uppsala University, Uppsala, Sweden.
Niklas
N
Marklund
Department of Neuroscience, Neurosurgery, Uppsala University Hospital, Uppsala University, Uppsala, Sweden.
Fredrik
F
Clausen
Biomolecular Imaging and Proteomics, Dept. of Pharmaceutical Biosciences, Uppsala University, Box 591 BMC, SE-75124 Uppsala, Sweden.
Patrik
P
Källback
Biomolecular Imaging and Proteomics, Dept. of Pharmaceutical Biosciences, Uppsala University, Box 591 BMC, SE-75124 Uppsala, Sweden.
Henrik
H
Loden
TU Wien, Institute of Chemical Technologies and Analytics, Vienna, Austria.
Andreas
A
Limbeck
Biomolecular Imaging and Proteomics, Dept. of Pharmaceutical Biosciences, Uppsala University, Box 591 BMC, SE-75124 Uppsala, Sweden. Electronic address: per.andren@farmbio.uu.se.
Per E
PE
Andrén
D000818:Animals; D000070642:Brain Injuries, Traumatic / Q000000981:diagnostic imaging* / Q000378:metabolism; D002118:Calcium / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D007668:Kidney / Q000000981:diagnostic imaging; D053685:Laser Therapy / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D051381:Rats; D012964:Sodium / Q000302:isolation & purification / Q000378:metabolism
D012964:Sodium; D002118:Calcium
Brain; Elemental imaging; Inductively coupled plasma; Kidney; Laser ablation; Mass spectrometry