Derivation of hybrid ES cell lines from two different strains of mice.
Parental origin-dependent expression of the imprinted genes is essential for mammalian development. Zfp57 maintains genomic imprinting in mouse embryos and ES cells. To examine the allelic expression patterns of the imprinted genes in ES cells, we obtained multiple hybrid ES clones that were directly derived from the blastocysts generated from the cross between mice on two different genetic backgrounds. The blastocyst-derived ES clones displayed largely intact DNA methylation imprint at the tested imprinted regions. These hybrid ES clones will be useful for future studies to examine the allelic expression of the imprinted genes in ES cells and their differentiated progeny.
Black Family Stem Cell Institute, Department of Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, One Gustave L. Levy Place, New York, NY 10029, USA.
Ho-Tak
HT
Lau
Lizhi
L
Liu
Chelsea
C
Ray
Fong T
FT
Bell
Xiajun
X
Li
D000818:Animals; D001755:Blastocyst / Q000166:cytology; D002460:Cell Line; D019175:DNA Methylation; D058732:Embryoid Bodies / Q000166:cytology; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D005838:Genotype; D006579:Heterozygote; D051379:Mice; D008811:Mice, Inbred DBA; D008856:Microscopy, Fluorescence; D060888:Real-Time Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D014157:Transcription Factors