Lead selenide nanoparticles-induced oxidative damage of kidney in rats.
OBJECTIVE
To investigate the effect of lead selenide nanoparticles (nano PbSe) on kidney in rats.


METHODS
Specific pathogen free SD rats were randomly divided into 4 groups (8 rats/group), and injected with of 0mg/kg (control group), 10mg/kg (low dose group), 20mg/kg (middle dose group), or 30mg/kg (high dose group) nano PbSe respectively. Seven weeks after injection, the serum was taken from rats for the detection of blood urea nitrogen (BUN), creatinine (Cr) and uric acid (UA). Superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA) and total antioxidant capacity (T-AOC) levels were detected using renal tissue homogenate. Pathological examination was performed on kidney sections.


RESULTS
The levels of BUN and Cr in three exposure groups were significantly increased compared with those of control group. Levels of UA in middle dose and high dose group were higher than those in the control group. Levels of SOD, GSH-Px and T-AOC in three exposure groups were markedly decreased compared with those in the control group. Levels of MDA in three exposure groups were higher than those in the control group. Pathological changes at different levels of kidneys were observed, and the damage was more serious with the increase of concentration.


CONCLUSIONS
Nano PbSe can lead to oxidative damage to the kidney, with the toxicity positively correlates to the dosage.
School of Public Health, North China University of Science and Technology, Tangshan, Hebei 063000, China.
Luping
L
Mao
School of Public Health, North China University of Science and Technology, Tangshan, Hebei 063000, China.
Qingzeng
Q
Qian
School of Public Health, North China University of Science and Technology, Tangshan, Hebei 063000, China.
Qingzhao
Q
Li
School of Public Health, North China University of Science and Technology, Tangshan, Hebei 063000, China.
Sihui
S
Wei
School of Public Health, North China University of Science and Technology, Tangshan, Hebei 063000, China.
Yanhua
Y
Cao
School of Public Health, North China University of Science and Technology, Tangshan, Hebei 063000, China.
Yulan
Y
Hao
School of Public Health, North China University of Science and Technology, Tangshan, Hebei 063000, China.
Nan
N
Liu
School of Public Health, North China University of Science and Technology, Tangshan, Hebei 063000, China.
Qian
Q
Wang
School of Public Health, North China University of Science and Technology, Tangshan, Hebei 063000, China.
Yuping
Y
Bai
School of Public Health, North China University of Science and Technology, Tangshan, Hebei 063000, China. Electronic address: zhengying0918@aliyun.com.
Guoying
G
Zheng
D000818:Animals; D001806:Blood Urea Nitrogen; D003404:Creatinine / Q000097:blood; D005979:Glutathione Peroxidase / Q000378:metabolism; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D007854:Lead / Q000633:toxicity*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D053768:Metal Nanoparticles / Q000633:toxicity* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D018384:Oxidative Stress / Q000187:drug effects; D017207:Rats, Sprague-Dawley; D018036:Selenium Compounds / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism
D018036:Selenium Compounds; C088065:lead selenide; D007854:Lead; D008315:Malondialdehyde; D003404:Creatinine; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
Creatinine; Oxidative damage; PbSe nanoparticles; Urea nitrogen