Salivary mRNA markers having the potential to detect oral squamous cell carcinoma segregated from oral leukoplakia with dysplasia.
In the current study the presence of extracellular IL-1B, IL-8, OAZ and SAT mRNAs in the saliva was evaluated as a tool in the early detection of oral squamous cell carcinoma.



34 patients with primary oral squamous cell carcinoma stage T1N0M0/T2N0M0, 20 patients with oral leukoplakia and dysplasia (15 patients with mild dysplasia and 5 with severe dysplasia/in situ carcinoma) and 31 matched healthy-control subjects were included in the study. The presence of IL-1B, IL-8, OAZ and SAT mRNA was evaluated in extracellular RNA isolated from saliva samples using sequence-specific primers and real-time RT-PCR. ROC curve analysis was used to estimate the ability of the biomarkers to detect oral squamous cell carcinoma patients.



The data reveal that the combination of these four biomarkers provides a good predictive probability of up to 80% (AUC=0.799, p=0.002) for patients with oral squamous cell carcinoma but not patients suffering from oral leukoplakia with dysplasia. Moreover, the combination of only the two biomarkers (SAT and IL-8) also raises a high predictive ability of 75.5% (AUC=0.755, p=0.007) approximately equal to the four biomarkers suggesting the use of the two biomarkers only in the prediction model for oral squamous cell carcinoma patients limiting the economic and health cost in half.



SAT and IL-8 mRNAs are present in the saliva in high quality and quantity, with a good discriminatory ability for oral squamous cell carcinoma patients only but not for patients with oral leukoplakia and dysplasia an oral potentially malignant disorder.
Department of Oral Medicineand Oral Pathology, School of Dentistry, Aristotle University, 54124 Thessaloniki, Greece. Electronic address: eumixailidou@yahoo.com.
Evangelia
E
Michailidou
Laboratory of Biological Chemistry, School of Medicine, Aristotle University, 54124 Thessaloniki, Greece. Electronic address: tzimagio@med.auth.gr.
Georgios
G
Tzimagiorgis
Laboratory of Biological Chemistry, School of Medicine, Aristotle University, 54124 Thessaloniki, Greece. Electronic address: fchatzop@auth.gr.
Fani
F
Chatzopoulou
Department of Oral and Maxillofacial Surgery, School of Dentistry, Aristotle University, 54124 Thessaloniki, Greece. Electronic address: vaxtseva@otenet.gr.
Konstantinos
K
Vahtsevanos
Department of Oral and Maxillofacial Surgery, School of Dentistry, Aristotle University, 54124 Thessaloniki, Greece. Electronic address: antoniad@dent.auth.gr.
Konstantinos
K
Antoniadis
Laboratory of Biological Chemistry, School of Medicine, Aristotle University, 54124 Thessaloniki, Greece. Electronic address: kouidou@auth.gr.
Sofia
S
Kouidou
Department of Oral Medicineand Oral Pathology, School of Dentistry, Aristotle University, 54124 Thessaloniki, Greece. Electronic address: anmark@dent.auth.gr.
Anastasios
A
Markopoulos
Department of Oral Medicineand Oral Pathology, School of Dentistry, Aristotle University, 54124 Thessaloniki, Greece. Electronic address: med@auth.gr.
Dimitrios
D
Antoniades
D014408:Biomarkers, Tumor / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D005260:Female; D006801:Humans; D007972:Leukoplakia, Oral / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism*; D012463:Saliva / Q000378:metabolism*
D014408:Biomarkers, Tumor; D012333:RNA, Messenger
Dysplasia; IL-1B; IL-8; In situ carcinoma (in situ Ca); OAZ; Oral leukoplakia; Oral squamous cell carcinoma (OSCC); SAT; Saliva; mRNA