Does MW Radiation Affect Gene Expression, Apoptotic Level, and Cell Cycle Progression of Human SH-SY5Y Neuroblastoma Cells?
Neuroblastoma (NB) is a cancer that occurs in sympathetic nervous system arising from neuroblasts and nerve tissue of the adrenal gland, neck, chest, or spinal cord. It is an embryonal malignancy and affects infants and children. In this study, we investigated the effects of microwave (MW) radiation on apoptotic activity, cell viability, and cell cycle progression in human SH-SY5Y NB cells which can give information about MW radiation effects on neural cells covering the period from the embryonic stages to infants. SH-SY5Y NB cells were exposed to 2.1 GHz W-CDMA modulated MW radiation for 24 h at a specific absorption rate of 0.491 W/kg. Control samples were in the same conditions with MW-exposed samples but they were not exposed to MW radiation. The apoptotic activity of cells was measured by Annexin-V-FITC and propidium iodide staining. Moreover, mRNA levels of proliferative and cell cycle proteins were determined by real-time RT-PCR. The change in cell cycle progression was observed by using CycleTest-Plus DNA reagent. No significant change was observed in apoptotic activity of MW-exposed cells compared to control cells. The mRNA levels of c-myc and cyclin D1 were significantly reduced in MW group (p < 0.05). The percentage of MW-exposed cells in G1 phase was significantly higher than the percentage of control cells in G1 phase. MW radiation caused cell cycle arrest in G1 phase. These results showed that 2.1 GHz W-CDMA modulated MW radiation did not cause apoptotic cell death but changed cell cycle progression.
Department of Adult Hematology, Faculty of Medicine, Gazi University, Besevler, Ankara, Turkey. kayhanhandan@gmail.com.
Handan
H
Kayhan
Department of Biophysics, Faculty of Medicine, Gazi University, Besevler, Ankara, Turkey.
Meric Arda
MA
Esmekaya
Department of Medical Biology and Genetics, Faculty of Medicine, Gazi University, Besevler, Ankara, Turkey.
Atiye Seda Yar
AS
Saglam
Department of Biophysics, Faculty of Medicine, Gazi University, Besevler, Ankara, Turkey.
Mehmed Zahid
MZ
Tuysuz
Department of Biophysics, Faculty of Medicine, Gazi University, Besevler, Ankara, Turkey.
Ayşe Gulnihal
AG
Canseven
Department of Adult Hematology, Faculty of Medicine, Gazi University, Besevler, Ankara, Turkey.
Abdullah Munci
AM
Yagci
Department of Biophysics, Faculty of Medicine, Gazi University, Besevler, Ankara, Turkey.
Nesrin
N
Seyhan
D017209:Apoptosis / Q000528:radiation effects*; D002453:Cell Cycle / Q000528:radiation effects*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000528:radiation effects; D015972:Gene Expression Regulation, Neoplastic / Q000528:radiation effects*; D006801:Humans; D008872:Microwaves; D009447:Neuroblastoma / Q000473:pathology*
Apoptosis; Cell cycle; Microwave radiation; Neuroblastoma; SH-SY5Y