Murine fundus fluorescein angiography: An alternative approach using a handheld camera.
In today's modern pharmacologic approach to treating sight-threatening retinal vascular disorders, there is an increasing demand for a compact, mobile, lightweight and cost-effective fluorescein fundus camera to document the effects of antiangiogenic drugs on laser-induced choroidal neovascularization (CNV) in mice and other experimental animals. We have adapted the use of the Kowa Genesis Df Camera to perform Fundus Fluorescein Angiography (FFA) in mice. The 1 kg, 28 cm high camera has built-in barrier and exciter filters to allow digital FFA recording to a Compact Flash memory card. Furthermore, this handheld unit has a steady Indirect Lens Holder that firmly attaches to the main unit, that securely holds a 90 diopter lens in position, in order to facilitate appropriate focus and stability, for photographing the delicate central murine fundus. This easily portable fundus fluorescein camera can effectively record exceptional central retinal vascular detail in murine laser-induced CNV, while readily allowing the investigator to adjust the camera's position according to the variable head and eye movements that can randomly occur while the mouse is optimally anesthetized. This movable image recording device, with efficiencies of space, time, cost, energy and personnel, has enabled us to accurately document the alterations in the central choroidal and retinal vasculature following induction of CNV, implemented by argon-green laser photocoagulation and disruption of Bruch's Membrane, in the experimental murine model of exudative macular degeneration.
Institute of Drug Research, School of Pharmacy, Faculty of Medicine, Hadassah Ein Kerem Medical Campus, Hebrew University of Jerusalem, Jerusalem, Israel.
Moshe
M
Ehrenberg
Ruth and Bruce Rappaport Faculty of Medicine, Technion Rambam Bat-Galim Medical Campus, Technion-Israel Institute of Technology, Haifa, Israel.
Scott
S
Ehrenberg
Institute of Drug Research, School of Pharmacy, Faculty of Medicine, Hadassah Ein Kerem Medical Campus, Hebrew University of Jerusalem, Jerusalem, Israel.
Ouri
O
Schwob
Institute of Drug Research, School of Pharmacy, Faculty of Medicine, Hadassah Ein Kerem Medical Campus, Hebrew University of Jerusalem, Jerusalem, Israel. Electronic address: OfraB@ekmd.huji.ac.il.
Ofra
O
Benny
D020533:Angiogenesis Inhibitors / Q000627:therapeutic use; D000818:Animals; D002829:Choroid / Q000098:blood supply*; D020256:Choroidal Neovascularization / Q000000981:diagnostic imaging* / Q000188:drug therapy; D004195:Disease Models, Animal; D005451:Fluorescein Angiography / Q000295:instrumentation* / Q000379:methods; D007091:Image Processing, Computer-Assisted / Q000379:methods; D051379:Mice; D010781:Photography / Q000379:methods*
D020533:Angiogenesis Inhibitors
Angiography; Experimental animal model; Imaging; Macula; Retina