Inhibition of Mitochondrial Voltage-Dependent Anion Channels Increases Radiosensitivity of K562 Leukemic Cells.
We studied the effect of inhibition of mitochondrial voltage-dependent anion channels with DIDS on radiosensitivity and mitochondrial status of K562 leukemic cells. The number of apoptotic and necrotic cells, mitochondrial transmembrane potential, and mitochondrial mass were evaluated after irradiation of cells in doses of 4 and 12 Gy in the presence and absence of the inhibitor. Inhibition of mitochondrial voltage-dependent anion channels increased radiosensitivity of K562 cells by 50-70% and decreased both mitochondrial transmembrane potential and mitochondrial mass. Inhibitors of voltage-dependent anion channels are promising agents capable of improving the effectiveness of cancer radiotherapy.
Laboratory of Molecular and Cell Biology, S. P. Kapitsa Research Technology Institute, Ulyanovsk State University, Ulyanovsk, Russia. saenkoyv@yandex.ru.
Y V
YV
Saenko
Laboratory of Molecular and Cell Biology, S. P. Kapitsa Research Technology Institute, Ulyanovsk State University, Ulyanovsk, Russia.
A V
AV
Mastilenko
Laboratory of Molecular and Cell Biology, S. P. Kapitsa Research Technology Institute, Ulyanovsk State University, Ulyanovsk, Russia.
E S
ES
Glushchenko
Laboratory of Molecular and Cell Biology, S. P. Kapitsa Research Technology Institute, Ulyanovsk State University, Ulyanovsk, Russia.
A V
AV
Antonova
Laboratory of Molecular and Cell Biology, S. P. Kapitsa Research Technology Institute, Ulyanovsk State University, Ulyanovsk, Russia.
V P
VP
Svekolkin
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D020014:K562 Cells; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D033681:Mitochondrial Membrane Transport Proteins / Q000037:antagonists & inhibitors*; D011836:Radiation Tolerance / Q000187:drug effects*; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D050994:Voltage-Dependent Anion Channels / Q000037:antagonists & inhibitors*
D033681:Mitochondrial Membrane Transport Proteins; D011838:Radiation-Sensitizing Agents; D050994:Voltage-Dependent Anion Channels; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
X-irradiation; mitochondrial mass; mitochondrial transmembrane potential; radiosensitivity; voltage-dependent anion channels