The Effect of Vibration Treatments Combined with Teriparatide or Strontium Ranelate on Bone Healing and Muscle in Ovariectomized Rats.
The aim of the present study was to study the effect of combined therapy of teriparatide (PTH) or strontium ranelate (SR) with whole-body vibration (WBV) on bone healing and muscle properties in an osteopenic rat model. Seventy-two rats (3 months old) were bilaterally ovariectomized (Ovx), and 12 rats were left intact (Non-Ovx). After 8 weeks, bilateral transverse osteotomy was performed at the tibia metaphysis in all rats. Thereafter, Ovx rats were divided into six groups (n = 12): (1) Ovx-no treatment, (2) Ovx + vibration (Vib), (3) SR, (4) SR + Vib, (5) PTH, and (6) PTH + Vib. PTH (40 μg/kg BW sc. 5×/week) and SR (613 mg/kg BW in food daily) were applied on the day of ovariectomy, vibration treatments 5 days later (vertical, 70 Hz, 0.5 mm, 2×/day for 15 min) for up to 6 weeks. In the WBV + SR group, the callus density, trabecular number, and Alp and Oc gene expression were decreased compared to SR alone. In the WBV + PTH group, the cortical and callus widths, biomechanical properties, Opg gene expression, and Opg/Rankl ratio were increased; the cortical and callus densities were decreased compared to PTH alone. A case of non-bridging was found in both vibrated groups. Vibration alone did not change the bone parameters; PTH possessed a stronger effect than SR therapy. In muscles, combined therapies improved the fiber size of Ovx rats. WBV could be applied alone or in combination with anti-osteoporosis drug therapy to improve muscle tissue. However, in patients with fractures, anti-osteoporosis treatments and the application of vibration could have an adverse effect on bone healing.
Department of Trauma Surgery and Reconstructive Surgery, University Medicine of Goettingen, Robert-Koch Str. 40, 37075, Göttingen, Germany. komrakova@yahoo.com.
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Komrakova
Department of Trauma Surgery and Reconstructive Surgery, University Medicine of Goettingen, Robert-Koch Str. 40, 37075, Göttingen, Germany.
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Hoffmann
Department of Trauma Surgery and Reconstructive Surgery, University Medicine of Goettingen, Robert-Koch Str. 40, 37075, Göttingen, Germany.
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Nuehnen
Department of Trauma Surgery and Reconstructive Surgery, University Medicine of Goettingen, Robert-Koch Str. 40, 37075, Göttingen, Germany.
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Stueber
Department of Medical Microbiology, Subdivision of General Hygiene and Environmental Health, University of Goettingen, Humboldallee 34a, 37073, Göttingen, Germany.
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Wassmann
Department of Animal Sciences, University of Goettingen, Albrecht-Thaer-Weg 3, 37075, Göttingen, Germany.
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Wicke
Department of Trauma Surgery and Reconstructive Surgery, University Medicine of Goettingen, Robert-Koch Str. 40, 37075, Göttingen, Germany.
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Tezval
Department of Trauma Surgery and Reconstructive Surgery, University Medicine of Goettingen, Robert-Koch Str. 40, 37075, Göttingen, Germany.
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Stuermer
Department of Trauma Surgery and Reconstructive Surgery, University Medicine of Goettingen, Robert-Koch Str. 40, 37075, Göttingen, Germany.
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Sehmisch
D000818:Animals; D001835:Body Weight; D015519:Bone Density / Q000187:drug effects; D050071:Bone Density Conservation Agents / Q000627:therapeutic use*; D001842:Bone and Bones / Q000378:metabolism / Q000473:pathology; D005260:Female; D017102:Fracture Healing / Q000187:drug effects; D050723:Fractures, Bone / Q000188:drug therapy; D010024:Osteoporosis / Q000188:drug therapy*; D010027:Osteotomy; D010052:Ovariectomy; D051381:Rats; D017207:Rats, Sprague-Dawley; D019379:Teriparatide / Q000008:administration & dosage*; D013876:Thiophenes / Q000008:administration & dosage*; D014732:Vibration / Q000627:therapeutic use*
D050071:Bone Density Conservation Agents; D013876:Thiophenes; C081587:strontium ranelate; D019379:Teriparatide
Bone healing; Combined therapy; Muscle structure; Strontium ranelate; Teriparatide; Whole-body vibration