Diffuse Optical Spectroscopy for the Quantitative Assessment of Acute Ionizing Radiation Induced Skin Toxicity Using a Mouse Model.
Acute skin toxicities from ionizing radiation (IR) are a common side effect from therapeutic courses of external beam radiation therapy (RT) and negatively impact patient quality of life and long term survival. Advances in the understanding of the biological pathways associated with normal tissue toxicities have allowed for the development of interventional drugs, however, current response studies are limited by a lack of quantitative metrics for assessing the severity of skin reactions. Here we present a diffuse optical spectroscopic (DOS) approach that provides quantitative optical biomarkers of skin response to radiation. We describe the instrumentation design of the DOS system as well as the inversion algorithm for extracting the optical parameters. Finally, to demonstrate clinical utility, we present representative data from a pre-clinical mouse model of radiation induced erythema and compare the results with a commonly employed visual scoring. The described DOS method offers an objective, high through-put evaluation of skin toxicity via functional response that is translatable to the clinical setting.
Department of Radiation Oncology, University of Toronto; Department of Physics, Ryerson University; lee.chin@sunnybrook.ca.
Lee
L
Chin
Department of Medical Biophysics, University of Toronto.
Elina
E
Korpela
Department of Radiation Oncology, University of Toronto.
Anthony
A
Kim
Department of Physics, Ryerson University.
Darren
D
Yohan
Ontario Cancer Institute / Campbell Family Institute for Cancer Research.
Carolyn
C
Niu
Department of Medical Biophysics, University of Toronto.
Brian C
BC
Wilson
Department of Radiation Oncology, University of Toronto; Department of Medical Biophysics, University of Toronto.
Stanley K
SK
Liu
D000818:Animals; D004195:Disease Models, Animal; D004890:Erythema / Q000209:etiology / Q000473:pathology; D051379:Mice; D011788:Quality of Life; D011839:Radiation, Ionizing; D011855:Radiodermatitis / Q000209:etiology / Q000473:pathology*; D012867:Skin / Q000528:radiation effects*; D013057:Spectrum Analysis / Q000379:methods*