Inhibition of long non-coding RNA TUG1 on gastric cancer cell transference and invasion through regulating and controlling the expression of miR-144/c-Met axis.
OBJECTIVE
To discuss the expression of long noncoding RNA TUG1 (lncRNA-TUG1) in gastric carcinoma (GC) and its effects on the transferring and invading capacity of gastric carcinoma cells.


METHODS
Forty cases of carcinoma tissue and para-carcinoma tissue were selected from GC patients who underwent surgical removal in Zhejiang Provincial Hospital of Chinese Traditional Medicine and Wenzhou Central Hospital from January, 2013 to December, 2014; the expressing level of lncRNA-TUG1 in GC and para-C tissues was detected by applying the qRT-PCR technique. The correlation between lncRNA-TUG1 expression and patients' clinical data was classified and analyzed. SGC-7901 cells were transfected using lncRNA-TUG1 specific siRNA. Changes of the transferring and invading capacity of siRNA-transfected SGC-7901 cells were scratch-tested and transwell-detected. qRT-PCR was applied to detect the expression level of microRNA-144 after lncRNA-TUG1 was silenced. Changes of c-Met mRNA and protein expressions was detected by qRT-PCR and western-blot test.


RESULTS
The expression level of lncRNA-TUG1 in GC tissue was significant higher than that in para-C tissue (P < 0.05) and the high expression level of lncRNA-TUG1 in GC tissue was significantly correlated with tumor lymph nodes metastasis and advance TNM phasing (P < 0.05). The transferring and invading capacity of SGC-7901 cells was highly inhibited after being transfected by lncRNA-TUG1 specific siRNA (P < 0.05). The results of qRT-PCR and western-blot proved that the expression of microRNA-144 was significantly boosted and the expression level of c-Met mRNA and protein was inhibited after lncRNA-TUG1 was silenced (P < 0.05).


CONCLUSIONS
lncRNA-TUG1 shows an up-regulated expression in GC tissue and that bears a correlation with clinicopathological features of malignant tumor. lncRNA-TUG1 can promote the transferring and invading capacity of GC by inhibiting the pathway of microRNA-144/c-Met.
Department of Gastroenterology, The Third Affiliated Hospital of Wenzhou Medical University (Ruian People's Hospital), Ruian 325200, Zhejiang, China. Electronic address: jitingting929@163.com.
Ting-Ting
TT
Ji
Department of Gastroenterology, Zhejiang Provincial Hospital of Chinese Traditional Medicine, Hangzhou 310006, Zhejiang, China.
Xuan
X
Huang
Department of Gastroenterology, Wenzhou Central Hospital, Wenzhou 325000, Zhejiang, China.
Jie
J
Jin
Department of Pathology, The Third Affiliated Hospital of Wenzhou Medical University (Ruian People's Hospital), Ruian 325200, Zhejiang, China.
Sheng-Hua
SH
Pan
Department of Gastroenterology, The Third Affiliated Hospital of Wenzhou Medical University (Ruian People's Hospital), Ruian 325200, Zhejiang, China.
Xiao-Ju
XJ
Zhuge
Gastric carcinoma; Invasion; Transference; c-Met; lncRNA-TUG1; microRNA-144