Increased fatty acid synthesis inhibits nitrogen starvation-induced autophagy in lipid droplet-deficient yeast.
Macroautophagy is a degradative pathway whereby cells encapsulate and degrade cytoplasmic material within endogenously-built membranes. Previous studies have suggested that autophagosome membranes originate from lipid droplets. However, it was recently shown that rapamycin could induce autophagy in cells lacking these organelles. Here we show that lipid droplet-deprived cells are unable to perform autophagy in response to nitrogen-starvation because of an accelerated lipid synthesis that is not observed with rapamycin. Using cerulenin, a potent inhibitor of fatty acid synthase, and exogenous addition of palmitic acid we could restore nitrogen-starvation induced autophagy in the absence of lipid droplets.
Laboratoire Signalisation & Transports Ioniques Membranaires (STIM) - CNRS ERL 7368, Université de Poitiers, Bat. B36, TSA 51106, 1 rue Georges Bonnet, 86073 Poitiers Cedex 9, France. Electronic address: matthieu.regnacq@univ-poitiers.fr.
Matthieu
M
Régnacq
Laboratoire Signalisation & Transports Ioniques Membranaires (STIM) - CNRS ERL 7368, Université de Poitiers, Bat. B36, TSA 51106, 1 rue Georges Bonnet, 86073 Poitiers Cedex 9, France.
Pierre
P
Voisin
Laboratoire Signalisation & Transports Ioniques Membranaires (STIM) - CNRS ERL 7368, Université de Poitiers, Bat. B36, TSA 51106, 1 rue Georges Bonnet, 86073 Poitiers Cedex 9, France.
Yves Y
YY
Sere
Laboratoire Signalisation & Transports Ioniques Membranaires (STIM) - CNRS ERL 7368, Université de Poitiers, Bat. B36, TSA 51106, 1 rue Georges Bonnet, 86073 Poitiers Cedex 9, France.
Bin
B
Wan
Laboratoire Signalisation & Transports Ioniques Membranaires (STIM) - CNRS ERL 7368, Université de Poitiers, Bat. B36, TSA 51106, 1 rue Georges Bonnet, 86073 Poitiers Cedex 9, France.
Venty M S
VMS
Soeroso
Laboratoire Signalisation & Transports Ioniques Membranaires (STIM) - CNRS ERL 7368, Université de Poitiers, Bat. B36, TSA 51106, 1 rue Georges Bonnet, 86073 Poitiers Cedex 9, France.
Marianne
M
Bernard
CNRS, IBGC, UMR 5095, 1 rue Camille Saint-Saëns, 33000 Bordeaux, France; Université de Bordeaux, IBGC, UMR5095, 1 rue Camille Saint-Saëns, 33000 Bordeaux, France.
Nadine
N
Camougrand
BIOalternatives, 1 bis rue des Plantes, 86160 Gençay, France.
François-Xavier
FX
Bernard
BIOalternatives, 1 bis rue des Plantes, 86160 Gençay, France.
Christine
C
Barrault
Laboratoire Signalisation & Transports Ioniques Membranaires (STIM) - CNRS ERL 7368, Université de Poitiers, Bat. B36, TSA 51106, 1 rue Georges Bonnet, 86073 Poitiers Cedex 9, France.
Thierry
T
Bergès
D001343:Autophagy; D005227:Fatty Acids / Q000096:biosynthesis*; D050356:Lipid Metabolism; D009584:Nitrogen / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D005227:Fatty Acids; D009584:Nitrogen
Autophagy; Fatty acid; Fatty acid synthase; Lipid droplets; Rapamycin; Saccharomyces cerevisiae