Taurine protects against As2O3-induced autophagy in livers of rat offsprings through PPARγ pathway.
Chronic exposures to arsenic had been associated with metabolism diseases. Peroxisome proliferator-activated receptor gamma (PPARγ) was found in the liver, regulated metabolism. Here, we found that the expression of PPARγ was decreased, the generation of reactive oxygen species (ROS) and autophagy were increased after treatment with As2O3 in offsprings' livers. Taurine (Tau), a sulfur-containing β-amino acid could reverse As2O3-inhibited PPARγ. Tau also inhibit the generation of ROS and autophagy. We also found that As2O3 caused autophagic cell death and ROS accelerated in HepG2 cells. Before incubation with As2O3, the cells were pretreated with PPARγ activator Rosiglitazone (RGS), we found that autophagy and ROS was inhibited in HepG2 cells, suggesting that inhibition of PPARγ contributed to As2O3-induced autophagy and the generation of ROS. After pretreatment with Tau, the level of PPARγ was improved and the autophagy and ROS was inhibited in As2O3-treated cells, suggesting that Tau could protect hepatocytes against As2O3 through modulating PPARγ pathway.
Department of Occupational and Environmental Health, Dalian Medical University, 9WLvshun South Road, Dalian 116044, PR China.
Jie
J
Bai
Department of Occupational and Environmental Health, Dalian Medical University, 9WLvshun South Road, Dalian 116044, PR China.
Xiaofeng
X
Yao
Liaoning Anti-Degenerative Diseases Natural Products Engineering Research Center, Dalian Medical University, 9WLvshun South Road, Dalian 116044, PR China.
Liping
L
Jiang
Department of Occupational and Environmental Health, Dalian Medical University, 9WLvshun South Road, Dalian 116044, PR China.
Qiaoting
Q
Zhang
Department of Obstetrics and Gynecology, NO. 210 Hospital of PLA, Dalian 116021, PR China.
Huai
H
Guan
Department of Occupational and Environmental Health, Dalian Medical University, 9WLvshun South Road, Dalian 116044, PR China.
Shuang
S
Liu
Department of Occupational and Environmental Health, Dalian Medical University, 9WLvshun South Road, Dalian 116044, PR China.
Wei
W
Wu
Department of Occupational and Environmental Health, Dalian Medical University, 9WLvshun South Road, Dalian 116044, PR China.
Tianming
T
Qiu
Department of Occupational and Environmental Health, Dalian Medical University, 9WLvshun South Road, Dalian 116044, PR China.
Ni
N
Gao
Department of Occupational and Environmental Health, Dalian Medical University, 9WLvshun South Road, Dalian 116044, PR China.
Lei
L
Yang
Liaoning Anti-Degenerative Diseases Natural Products Engineering Research Center, Dalian Medical University, 9WLvshun South Road, Dalian 116044, PR China.
Guang
G
Yang
Department of Occupational and Environmental Health, Dalian Medical University, 9WLvshun South Road, Dalian 116044, PR China.
Xiance
X
Sun
D000818:Animals; D000077237:Arsenic Trioxide; D001152:Arsenicals; D001343:Autophagy / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D015536:Down-Regulation / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D010087:Oxides / Q000633:toxicity*; D047495:PPAR gamma / Q000378:metabolism*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D000077154:Rosiglitazone; D015398:Signal Transduction / Q000187:drug effects; D013654:Taurine / Q000008:administration & dosage* / Q000494:pharmacology; D045162:Thiazolidinediones / Q000008:administration & dosage / Q000494:pharmacology
D001152:Arsenicals; D010087:Oxides; D047495:PPAR gamma; D017382:Reactive Oxygen Species; D045162:Thiazolidinediones; D000077154:Rosiglitazone; D013654:Taurine; D000077237:Arsenic Trioxide