LobeFinder: A Convex Hull-Based Method for Quantitative Boundary Analyses of Lobed Plant Cells.
Dicot leaves are composed of a heterogeneous mosaic of jigsaw puzzle piece-shaped pavement cells that vary greatly in size and the complexity of their shape. Given the importance of the epidermis and this particular cell type for leaf expansion, there is a strong need to understand how pavement cells morph from a simple polyhedral shape into highly lobed and interdigitated cells. At present, it is still unclear how and when the patterns of lobing are initiated in pavement cells, and one major technological bottleneck to addressing the problem is the lack of a robust and objective methodology to identify and track lobing events during the transition from simple cell geometry to lobed cells. We developed a convex hull-based algorithm termed LobeFinder to identify lobes, quantify geometric properties, and create a useful graphical output of cell coordinates for further analysis. The algorithm was validated against manually curated images of pavement cells of widely varying sizes and shapes. The ability to objectively count and detect new lobe initiation events provides an improved quantitative framework to analyze mutant phenotypes, detect symmetry-breaking events in time-lapse image data, and quantify the time-dependent correlation between cell shape change and intracellular factors that may play a role in the morphogenesis process.
Department of Agricultural and Biological Engineering (T.-C.W., J.P., D.M.U.), Department of Botany and Plant Pathology (S.A.B., D.B.S.), Department of Agronomy (D.B.S.), Department of Biological Sciences (D.B.S.), and Weldon School of Biomedical Engineering (D.M.U.), Purdue University, West Lafayette, Indiana 47907.
Tzu-Ching
TC
Wu
Department of Agricultural and Biological Engineering (T.-C.W., J.P., D.M.U.), Department of Botany and Plant Pathology (S.A.B., D.B.S.), Department of Agronomy (D.B.S.), Department of Biological Sciences (D.B.S.), and Weldon School of Biomedical Engineering (D.M.U.), Purdue University, West Lafayette, Indiana 47907.
Samuel A
SA
Belteton
Department of Agricultural and Biological Engineering (T.-C.W., J.P., D.M.U.), Department of Botany and Plant Pathology (S.A.B., D.B.S.), Department of Agronomy (D.B.S.), Department of Biological Sciences (D.B.S.), and Weldon School of Biomedical Engineering (D.M.U.), Purdue University, West Lafayette, Indiana 47907.
Jessica
0000-0001-5462-524X
J
Pack
Department of Agricultural and Biological Engineering (T.-C.W., J.P., D.M.U.), Department of Botany and Plant Pathology (S.A.B., D.B.S.), Department of Agronomy (D.B.S.), Department of Biological Sciences (D.B.S.), and Weldon School of Biomedical Engineering (D.M.U.), Purdue University, West Lafayette, Indiana 47907 dszyman@purdue.edu dumulis@purdue.edu.
Daniel B
DB
Szymanski
Department of Agricultural and Biological Engineering (T.-C.W., J.P., D.M.U.), Department of Botany and Plant Pathology (S.A.B., D.B.S.), Department of Agronomy (D.B.S.), Department of Biological Sciences (D.B.S.), and Weldon School of Biomedical Engineering (D.M.U.), Purdue University, West Lafayette, Indiana 47907 dszyman@purdue.edu dumulis@purdue.edu.
David M
DM
Umulis
D000465:Algorithms; D048430:Cell Shape; D018548:Cotyledon / Q000235:genetics / Q000648:ultrastructure; D009154:Mutation; D010641:Phenotype; D059828:Plant Cells / Q000648:ultrastructure*; D063245:Plant Development / Q000235:genetics; D018515:Plant Leaves / Q000235:genetics / Q000648:ultrastructure; D010944:Plants / Q000235:genetics / Q000648:ultrastructure*