Evaluation of the anti-inflammatory effects of volatile oils from processed products of Angelica sinensis radix by GC-MS-based metabolomics.
BACKGROUND
Roots of Angelica sinensis (Oliv.) Diels (AS) a commonly used herbal, always act as an anti-inflammatory drug in Chinese traditional therapy. In clinical use, AS is always processed before being used for the reason that processing can increase its therapeutic effect. Recent studies have shown that volatile oil of AS (VOAS), an important component in AS, has evident anti-inflammatory activities.


OBJECTIVE
In this study, our aim is to evaluate the anti-inflammatory effects of volatile oils from processed products of AS.


METHODS
In this paper, volatile oils from stir-fried AS (C-VOAS), parched AS with alcohol (J-VOAS), parched AS with soil (T-VOAS), and parched AS with sesame oil (Y-VOAS) were applied to intervene the carrageenan-induced acute inflammation model rats. GC-MS based metabolomics was utilized to determine different metabolites in the inflammatory exudate and plasma samples.


RESULTS
The results showed that VOASs could significantly inhibit the release of PGE2, HIS, 5-HT and TNF-α, among which C-VOAS and J-VOAS expressed better effect. Otherwise, 14 potential biomarkers were identified respectively in inflammatory exudate and plasma, which changed highly significantly (P<0.01) in C-VOAS and J-VOAS groups.


CONCLUSIONS
We inferred that the anti-inflammatory effect of C-VOAS and J-VOAS were superior to other VOASs.
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, Gansu 730070, People's Republic of China.
Li-Jia
LJ
Zhong
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, Gansu 730070, People's Republic of China.
Yong-Li
YL
Hua
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, Gansu 730070, People's Republic of China.
Peng
P
Ji
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, Gansu 730070, People's Republic of China.
Wan-Ling
WL
Yao
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, Gansu 730070, People's Republic of China.
Wen-Quan
WQ
Zhang
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, Gansu 730070, People's Republic of China.
Jian
J
Li
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, Gansu 730070, People's Republic of China. Electronic address: weiym@gsau.edu.cn.
Yan-Ming
YM
Wei
D029971:Angelica sinensis / Q000737:chemistry*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D015415:Biomarkers / Q000378:metabolism; D002351:Carrageenan; D015232:Dinoprostone / Q000378:metabolism; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D008401:Gas Chromatography-Mass Spectrometry; D006632:Histamine / Q000378:metabolism; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D018836:Inflammation Mediators / Q000378:metabolism; D008297:Male; D055432:Metabolomics / Q000379:methods*; D015999:Multivariate Analysis; D009822:Oils, Volatile / Q000302:isolation & purification / Q000494:pharmacology*; D008517:Phytotherapy; D010938:Plant Oils / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal; D025341:Principal Component Analysis; D017208:Rats, Wistar; D012701:Serotonin / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D015415:Biomarkers; D004365:Drugs, Chinese Herbal; D018836:Inflammation Mediators; D009822:Oils, Volatile; D010938:Plant Oils; D014409:Tumor Necrosis Factor-alpha; D012701:Serotonin; D006632:Histamine; D002351:Carrageenan; C044613:angelicae sinensis extract; D015232:Dinoprostone
5-hydroxytryptamine (PubChem CID: 5202); Aldosterone (PubChem CID: 5839); Angelica sinensis (Oliv.) Diels; Arachidonic acid (PubChem CID: 444899); Cholesterol (PubChem CID: 5997); D-Glucose (PubChem CID: 5793); Different processed products; Glycine (PubChem CID: 750); Inflammation; L-Histidine (PubChem CID: 6274); Lactic acid (PubChem CID: 612); Metabolomics; Prostaglandin E2 (PubChem CID: 5280360); Traditional Chinese medicine; Tryptophan (PubChem CID: 635); Volatile oils