The Ionic Products from Mineral Trioxide Aggregate-induced Odontogenic Differentiation of Dental Pulp Cells via Activation of the Wnt/β-catenin Signaling Pathway.
BACKGROUND
Mineral trioxide aggregate (MTA) has been successfully used in clinical applications in endodontics. However, little is known about the involvement of Wnt/β-catenin signaling in human dental pulp cells (hDPC) differentiation with the interaction of MTA in hard tissue regeneration, especially in odontogenesis. Therefore, the aim of this study was to explore odontogenic/osteogenic gene expression and the protein secretion of hDPCs cultured with consecutive concentrations of MTA extracts and carefully examine the particular molecular mechanism that occurs during this process.


METHODS
MTA extracts were prepared by immersing MTA powders into Dulbecco modified Eagle medium at a concentration of 200 mg/mL. hDPCs were cultured with various concentrations of MTA extracts, and the resulting changes in the cells, such as proliferation and odontogenic differentiation, were measured.


RESULTS
The results indicate that hDPC proliferation increases remarkably in a time-dependent manner in most treatment groups, except the highest concentration group (200 mg/mL). The Wnt/β-catenin signaling pathway-related genes and proteins are significantly raised when hDPCs are cultured in a wide concentration range of MTA extracts compared with a control, except for the highest concentration group (100 mg/mL), on days 3 and 7 (P < .05).


CONCLUSIONS
These variations indicate that Wnt/β-catenin signaling is involved in MTA extract-induced odontogenic differentiation of hDPCs.
3D Printing Medical Research Center, China Medical University Hospital, China Medical University, Taichung, Taiwan; Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan.
Yi-Wen
YW
Chen
3D Printing Medical Research Center, China Medical University Hospital, China Medical University, Taichung, Taiwan.
Chia-Che
CC
Ho
School of Dentistry, Chung Shan Medical University, Taichung, Taiwan; Department of Stomatology, Chung Shan Medical University Hospital, Taichung, Taiwan.
Tsui-Hsien
TH
Huang
3D Printing Medical Research Center, China Medical University Hospital, China Medical University, Taichung, Taiwan.
Tuan-Ti
TT
Hsu
3D Printing Medical Research Center, China Medical University Hospital, China Medical University, Taichung, Taiwan; School of Dentistry, China Medical University, Taichung, Taiwan. Electronic address: eviltacasi@gmail.com.
Ming-You
MY
Shie
D017607:Aluminum Compounds / Q000494:pharmacology*; D017610:Calcium Compounds / Q000494:pharmacology*; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D003782:Dental Pulp / Q000166:cytology*; D004338:Drug Combinations; D006801:Humans; D066298:In Vitro Techniques; D009805:Odontogenesis / Q000187:drug effects*; D010087:Oxides / Q000494:pharmacology*; D017640:Silicates / Q000494:pharmacology*; D060449:Wnt Signaling Pathway / Q000502:physiology*
D017607:Aluminum Compounds; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D017640:Silicates; C086631:mineral trioxide aggregate
Dental pulp cell; Wnt/β-catenin; ion concentration; mineral trioxide aggregate; odontogenic