Molecular characterization of a novel putative ampelovirus tentatively named grapevine leafroll-associated virus 13.
A novel putative ampelovirus was detected in grapevines that showed typical leafroll symptoms and was tentatively named grapevine leafroll-associated virus (GLRaV)-13 following the series of numbering of other GLRaVs. The complete genome of GLRaV-13 comprised 17,608 nt and contained eleven putative open reading frames, showing genetic features similar to those of viruses belonging to subgroup I of genus Ampelovirus. Phylogenetic trees based on the RNA-dependent RNA polymerase, heat shock protein 70 homolog, and coat protein showed that GLRaV-13 had the closest, but still distant, relationship to GLRaV-1 in the subgroup I cluster.
Grape and Persimmon Research Station, NARO Institute of Fruit Tree Science (NIFTS), National Agriculture and Food Research Organization (NARO), Akitsu, Hiroshima, 739-2494, Japan. itotaka@affrc.go.jp.
Takao
T
Ito
NIFTS, NARO, Tsukuba, Ibaraki, 305-8605, Japan.
Ryoji
R
Nakaune
D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D010942:Plant Viruses / Q000145:classification* / Q000235:genetics*; D027843:Vitis / Q000821:virology*