Chitosan based nanoparticles as protein carriers for efficient oral antigen delivery.
This study aimed to investigate the efficacy of nanoparticles based on chitosan as a vehicle for oral antigen delivery in fish vaccination. Carboxymethyl chitosan/chitosan nanoparticles (CMCS/CS-NPs) loaded extracellular products (ECPs) of Vibrio anguillarum were successfully developed by ionic gelation method. The prepared ECPs-loaded CMCS/CS-NPs were characterized for various parameters including morphology, particle size (312±7.18nm), zeta potential (+17.4±0.38mV), loading efficiency (57.8±2.54%) and stability under the simulated gastrointestinal (GI) tract conditions in turbot. The in vitro profile showed that the cumulative release of ECPs from nanoparticles was higher in pH 7.4 (58%) than in pH 2.0 (37%) and pH 4.5 (29%) after 48h. Fluorescein isothiocyanate-labeled bovine serum albumin (FITC-BSA) was used as model protein antigen and encapsulated in CMCS/CS-NPs for investigating the biodistribution of antigen after oral delivery to turbot in 24h. Oral immunization of ECPs-loaded CMCS/CS-NPs group in turbot showed elevated specific antibody and higher concentrations of lysozyme activity and complement activity in fish serum than ECPs solution. CMCS/CS-NPs loaded with ECPs could enhance both adaptive and innate immune responses than the group treated with ECPs solution and suggested to be a potential antigen delivery system.
College of Marine Life Science, Ocean University of China, Qingdao 266003, PR China.
Ping
P
Gao
College of Marine Life Science, Ocean University of China, Qingdao 266003, PR China.
Guixue
G
Xia
College of Marine Life Science, Ocean University of China, Qingdao 266003, PR China.
Zixian
Z
Bao
College of Marine Life Science, Ocean University of China, Qingdao 266003, PR China.
Chao
C
Feng
College of Marine Life Science, Ocean University of China, Qingdao 266003, PR China.
Xiaojie
X
Cheng
College of Marine Life Science, Ocean University of China, Qingdao 266003, PR China.
Ming
M
Kong
College of Marine Life Science, Ocean University of China, Qingdao 266003, PR China. Electronic address: yaliu@ouc.edu.cn.
Ya
Y
Liu
College of Marine Life Science, Ocean University of China, Qingdao 266003, PR China. Electronic address: xgchen@ouc.edu.cn.
Xiguang
X
Chen
D000284:Administration, Oral; D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry / Q000494:pharmacology; D001428:Bacterial Vaccines / Q000737:chemistry / Q000494:pharmacology; D002417:Cattle; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry / Q000494:pharmacology; D005393:Fish Diseases / Q000276:immunology / Q000517:prevention & control*; D005399:Fishes / Q000276:immunology*; D053758:Nanoparticles / Q000737:chemistry*; D012710:Serum Albumin, Bovine; D014733:Vibrio / Q000737:chemistry* / Q000276:immunology; D014735:Vibrio Infections / Q000276:immunology / Q000517:prevention & control*
D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D004337:Drug Carriers; D012710:Serum Albumin, Bovine; D048271:Chitosan
Adaptive and innate immune responses; CMCS/CS-NPs; ECPs; Oral immunization