A novel colorimetric and turn-on fluorescent chemosensor for iron(III) ion detection and its application to cellular imaging.
A novel rhodamine-based dual probe Rh-2 for trivalent ferric ions (Fe(3+)) was successfully designed and synthesized, which exhibited a highly sensitive and selective recognition towards Fe(3+) with an enhanced fluorescence emission in methanol-water media (v/v=7/3, pH=7.2). The probe Rh-2 could be applied to the determination of Fe(3+) with a linear range covering from 3.0×10(-7) to 1.4×10(-5)M and a detection limit of 1.24×10(-8)M. Meanwhile, the binding ratio of Rh-2 and Fe(3+) was found to be 1:1. Most importantly, the fluorescence and color signal changes of the Rh-2 solution were specific to Fe(3+) over other commonly coexistent metal ions. Moreover, the probe Rh-2 has been used to image Fe(3+) in living cells with satisfying results.
College of Chemistry and Chemical Engineering, University of South China, 28 Changsheng West Road Hengyang, Hunan 421001, PR China.
Aoheng
A
Luo
College of Chemistry and Chemical Engineering, University of South China, 28 Changsheng West Road Hengyang, Hunan 421001, PR China. Electronic address: hqwang2015@126.com.
Hongqing
H
Wang
College of Chemistry and Chemical Engineering, University of South China, 28 Changsheng West Road Hengyang, Hunan 421001, PR China.
Yuyuan
Y
Wang
College of Chemistry and Chemical Engineering, University of South China, 28 Changsheng West Road Hengyang, Hunan 421001, PR China.
Qiao
Q
Huang
College of Chemistry and Chemical Engineering, University of South China, 28 Changsheng West Road Hengyang, Hunan 421001, PR China.
Qin
Q
Zhang
D000818:Animals; D002412:Cations / Q000032:analysis; D002460:Cell Line; D005290:Ferric Compounds / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry*; D007501:Iron / Q000032:analysis*; D057230:Limit of Detection; D008856:Microscopy, Fluorescence / Q000379:methods; D061848:Optical Imaging / Q000379:methods*; D051381:Rats; D012235:Rhodamines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods
D002412:Cations; D005290:Ferric Compounds; D005456:Fluorescent Dyes; D012235:Rhodamines; D007501:Iron
Cell imaging; Ferric ion; Fluorescence enhancement; Fluorescent probe; Rhodamine