Joint effects of crude oil and heavy metals on the gill filament EROD activity of marbled rockfish Sebastiscus marmoratus.
The aim of this study was to characterize dose- and time-dependent responses of gill 7-ethoxyresorufin O-deethylase (EROD) activity from Juvenile marbled rockfish (Sebastiscus marmoratus) exposed to the water-accommodated fraction (WAF) of crude oil and heavy metal Cd(Ⅱ) or Pb(Ⅱ) alone or in mixture. Compared to the control group, gill filament EROD activity in S. marmoratus was significantly induced after exposure to the WAF from 80 to 320μg/L for 5 days in dose response experiment and after exposure to 40μg/L WAF for 6-10 days in time course experiment, respectively. In the other hand, gill filament EROD activity were not significantly affected compared to the control group or related WAF groups no matter in the dose response experiment or in the time course experiment of Cd(Ⅱ), Pb(Ⅱ) or its mixture with WAF. The results suggest the use of gill filament EROD activity as a biomarker of exposure to waterborne AhR agonists in marine ecosystems while simultaneously being exposed to environmental concentrations of Cd(Ⅱ) or Pb(Ⅱ).
Third Institute of Oceanography, State Oceanic Administration, Xiamen, Fujian Province, PR China.
Ronghui
R
Zheng
Xiaocuo Office of Quanzhou Entry-Exit Inspection & Quarantine Bureau of P.R.China, PR China.
Huanbin
H
Chen
Third Institute of Oceanography, State Oceanic Administration, Xiamen, Fujian Province, PR China.
Jun
J
Bo
Third Institute of Oceanography, State Oceanic Administration, Xiamen, Fujian Province, PR China.
Qing
Q
Xie
Third Institute of Oceanography, State Oceanic Administration, Xiamen, Fujian Province, PR China.
Fukun
F
Hong
Third Institute of Oceanography, State Oceanic Administration, Xiamen, Fujian Province, PR China. Electronic address: zhangyusheng@tio.org.cn.
Yusheng
Y
Zhang
D000818:Animals; D001492:Bass; D015415:Biomarkers / Q000378:metabolism; D002104:Cadmium / Q000633:toxicity*; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism*; D029941:Fish Proteins / Q000378:metabolism; D005880:Gills / Q000187:drug effects* / Q000201:enzymology; D007854:Lead / Q000633:toxicity*; D010578:Petroleum / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D015415:Biomarkers; D029941:Fish Proteins; D010578:Petroleum; D014874:Water Pollutants, Chemical; D002104:Cadmium; D007854:Lead; D019363:Cytochrome P-450 CYP1A1
7-ethoxyresorufin O-deethylase; Cadmium; Crude oil; Gill; Lead; Water-accommodated fraction