Probiotic Lactobacillus gasseri SBT2055 improves glucose tolerance and reduces body weight gain in rats by stimulating energy expenditure.
Probiotic Lactobacillus gasseri SBT2055 (LG2055) reduces postprandial TAG absorption and exerts anti-obesity effects in rats and humans; however, the underlying mechanisms are not fully understood. In the present study, we addressed the mechanistic insights of the anti-obesity activity of LG2055 by feeding Sprague-Dawley rats diets containing skimmed milk fermented or not by LG2055 for 4 weeks and by analysing energy expenditure, glucose tolerance, the levels of SCFA in the caecum and serum inflammatory markers. Rats fed the LG2055-containing diet demonstrated significantly higher carbohydrate oxidation in the dark cycle (active phase for rats) compared with the control group, which resulted in a significant increase in energy expenditure. LG2055 significantly reduced cumulative blood glucose levels (AUC) compared with the control diet after 3 weeks and increased the molar ratio of butyrate:total SCFA in the caecum after 4 weeks. Furthermore, the LG2055-supplemented diet significantly reduced the levels of serum amyloid P component - an indicator of the inflammatory process. In conclusion, our results demonstrate that, in addition to the inhibition of dietary TAG absorption reported previously, the intake of probiotic LG2055 enhanced energy expenditure via carbohydrate oxidation, improved glucose tolerance and attenuated inflammation, suggesting multiple additive and/or synergistic actions underlying the anti-obesity effects exerted by LG2055.
1Laboratory of Nutrition Chemistry,Department of Bioscience and Biotechnology, Faculty of Agriculture,Graduate School,Kyushu University,6-10-1 Hakozaki,Higashi-ku,Fukuoka 812-8581,Japan.
Bungo
B
Shirouchi
2Laboratory of Nutrition Biochemistry,Department of Applied Biochemistry and Food Science,Saga University,1 Honjo,Saga 840-8502,Japan.
Koji
K
Nagao
1Laboratory of Nutrition Chemistry,Department of Bioscience and Biotechnology, Faculty of Agriculture,Graduate School,Kyushu University,6-10-1 Hakozaki,Higashi-ku,Fukuoka 812-8581,Japan.
Minami
M
Umegatani
1Laboratory of Nutrition Chemistry,Department of Bioscience and Biotechnology, Faculty of Agriculture,Graduate School,Kyushu University,6-10-1 Hakozaki,Higashi-ku,Fukuoka 812-8581,Japan.
Aya
A
Shiraishi
1Laboratory of Nutrition Chemistry,Department of Bioscience and Biotechnology, Faculty of Agriculture,Graduate School,Kyushu University,6-10-1 Hakozaki,Higashi-ku,Fukuoka 812-8581,Japan.
Yukiko
Y
Morita
2Laboratory of Nutrition Biochemistry,Department of Applied Biochemistry and Food Science,Saga University,1 Honjo,Saga 840-8502,Japan.
Shunichi
S
Kai
3Faculty of Health and Nutrition Science,Nishikyushu University,4490-9 Ozaki,Kanzaki,Saga 842-8585,Japan.
Teruyoshi
T
Yanagita
4Milk Science Research Institute,Megmilk Snow Brand Co. Ltd,1-1-2 Minamidai,Kawagoe,Saitama 350-1165,Japan.
Akihiro
A
Ogawa
4Milk Science Research Institute,Megmilk Snow Brand Co. Ltd,1-1-2 Minamidai,Kawagoe,Saitama 350-1165,Japan.
Yukio
Y
Kadooka
1Laboratory of Nutrition Chemistry,Department of Bioscience and Biotechnology, Faculty of Agriculture,Graduate School,Kyushu University,6-10-1 Hakozaki,Higashi-ku,Fukuoka 812-8581,Japan.
Masao
M
Sato
D000818:Animals; D019440:Anti-Obesity Agents / Q000627:therapeutic use*; D019540:Area Under Curve; D001786:Blood Glucose / Q000378:metabolism*; D002087:Butyrates / Q000378:metabolism; D050260:Carbohydrate Metabolism; D002432:Cecum / Q000378:metabolism; D043302:Cultured Milk Products / Q000382:microbiology; D004032:Diet; D004734:Energy Metabolism; D005232:Fatty Acids, Volatile / Q000378:metabolism; D007249:Inflammation / Q000097:blood / Q000517:prevention & control; D000069998:Lactobacillus gasseri; D050356:Lipid Metabolism; D008297:Male; D009765:Obesity / Q000517:prevention & control*; D019936:Probiotics / Q000627:therapeutic use*; D017207:Rats, Sprague-Dawley; D000683:Serum Amyloid P-Component / Q000378:metabolism; D014280:Triglycerides / Q000097:blood; D015430:Weight Gain
D019440:Anti-Obesity Agents; D001786:Blood Glucose; D002087:Butyrates; D005232:Fatty Acids, Volatile; D000683:Serum Amyloid P-Component; D014280:Triglycerides
CRP C-reactive protein; Energy expenditure; GIP glucose-dependent insulinotropic polypeptide; GLP-1 glucagon-like peptide-1; GPR41 G protein-coupled receptors 41; Glucose tolerance; Lactobacillus gasseri SBT2055; Obesity; SAP serum amyloid P component; SM skimmed milk