Programmable DNA Nanosystem for Molecular Interrogation.
We describe a self-assembling DNA-based nanosystem for interrogating molecular interactions. The nanosystem contains a rigid supporting dumbbell-shaped frame, a cylindrical central core, and a mobile ring that is coaxial with the core. Motion of the ring is influenced by several control elements whose force-generating capability is based on the transition of single-stranded DNA to double-stranded DNA. These forces can be directed to act in opposition to adhesive forces between the ring and the frame thereby providing a mechanism for molecular detection and interrogation at the ring-frame interface. As proof of principle we use this system to evaluate base stacking adhesion and demonstrate detection of a soluble nucleic acid viral genome mimic.
Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA 50011, USA.|Bioinformatics and Computational Biology Program, Iowa State University, Ames, IA 50011, USA.|College of Science, George Mason University, Fairfax, USA.|Center for Bio/Molecular Science and Engineering, Code 6900, U.S. Naval Research Laboratory, Washington, USA.
Divita
D
Mathur
Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA 50011, USA.|Bioinformatics and Computational Biology Program, Iowa State University, Ames, IA 50011, USA.
Eric R
ER
Henderson
D004247:DNA / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures
D004247:DNA