Aberrant miR-181b-5p and miR-486-5p expression in serum and tissue of non-small cell lung cancer.
BACKGROUND
Lung cancer is the leading cause of cancer deaths in China. Non-small cell lung cancer (NSCLC) is the major type of lung cancer.


OBJECTIVE
The aim of our study was to characterize the expression profiles of miRNAs in serum and tissue of NSCLC at the same time, and to find more accurate relationship of miRNAs between serum and tissue. Furthermore, we intended to find more biomarkers of miRNAs in NSCLC samples.


METHODS
In this study, the miRNAs were sequenced in 18 paired serum and 18 paired tissue samples. The expression levels of miRNAs and targets were quantified by qRT-PCR. The function analysis was performed by using bioinformatics methods.


RESULTS
In these paired samples miR-181b-5p was up-regulated in squamous cell carcinoma (SCC), miR-486-5p was down-regulated in adenocarcinoma (AC), and miR-21-5p was up-regulated in both SCC and AC. However, miR-181b-5p and miR-486-5p were rarely reported in lung cancer related studies. The expression levels of these two miRNAs and their targets, RASSF1 and PIK3R1 were quantified in additional samples by qRT-PCR. The results showed that the targets were negatively regulated by the two miRNAs. In addition, we noted that RASSF1 and PIK3R1 were directly involved in non-small cell lung cancer pathway.


CONCLUSIONS
Our study suggested that miR-181b-5p and miR-486-5p could be new potential biomarkers for early diagnosis of NSCLC.
Research Center for Learning Science, Southeast University, Nanjing 210096, China; State Key Lab of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
Fei
F
Tian
Research Center for Learning Science, Southeast University, Nanjing 210096, China.
Yanting
Y
Shen
Research Center for Learning Science, Southeast University, Nanjing 210096, China.
Zhenzhu
Z
Chen
Research Center for Learning Science, Southeast University, Nanjing 210096, China.
Rui
R
Li
State Key Lab of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
Jiafeng
J
Lu
State Key Lab of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China. Electronic address: geqinyu@seu.edu.cn.
Qinyu
Q
Ge
D000230:Adenocarcinoma / Q000235:genetics; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics; D015536:Down-Regulation; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D012334:RNA, Neoplasm / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D015854:Up-Regulation
D014408:Biomarkers, Tumor; C560945:MIRN486 microRNA, human; C540193:MIrn181 microRNA, human; D035683:MicroRNAs; D012334:RNA, Neoplasm
Biomarker; MiR-181-5p; MiR-486-5p; MiRNA; NSCLC; Serum