Analysis of miRNAs and their target genes associated with lipid metabolism in duck liver.
Fat character is an important index in duck culture that linked to local flavor, feed cost and fat intake for costumers. Since the regulation networks in duck lipid metabolism had not been reported very clearly, we aimed to explore the potential miRNA-mRNA pairs and their regulatory roles in duck lipid metabolism. Here, Cherry-Valley ducks were selected and treated with/without 5% oil added in feed for 2 weeks, and then fat content determination was performed on. The data showed that the fat contents and the fatty acid ratios of C17:1 and C18:2 were up-regulated in livers of oil-added ducks, while the C12:0 ratio was down-regulated. Then 21 differential miRNAs, including 10 novel miRNAs, were obtain from the livers by sequencing, and 73 target genes involved in lipid metabolic processes of these miRNAs were found, which constituted 316 miRNA-mRNA pairs. Two miRNA-mRNA pairs including one novel miRNA and one known miRNA, N-miR-16020-FASN and gga-miR-144-ELOVL6, were selected to validate the miRNA-mRNA negative relation. And the results showed that N-mir-16020 and gga-miR-144 could respectively bind the 3'-UTRs of FASN and ELOVL6 to control their expressions. This study provides new sights and useful information for future research on regulation network in duck lipid metabolism.
Department of Animal Science, Wenzhou Vocational College of Science &Technology, Wenzhou 325006, P.R. China.|Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy Agricultural Sciences, Hangzhou 310021, P.R. China.
Jun
J
He
Department of Human Nutrition, Kansas State University, Manhattan, Kansas, 66506, USA.
Weiqun
W
Wang
Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy Agricultural Sciences, Hangzhou 310021, P.R. China.
Lizhi
L
Lu
Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy Agricultural Sciences, Hangzhou 310021, P.R. China.
Yong
Y
Tian
College of Animal Science, Zhejiang University, Hangzhou 310021, P.R. China.
Dong
D
Niu
Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy Agricultural Sciences, Hangzhou 310021, P.R. China.|Zhejiang Zhuowang Agriculture Sci-Tech Limited Co., Huzhou 313014, P.R. China.
Jindong
J
Ren
Department of Animal Science, Wenzhou Vocational College of Science &Technology, Wenzhou 325006, P.R. China.|Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy Agricultural Sciences, Hangzhou 310021, P.R. China.
Liyan
L
Dong
Department of Animal Science, Wenzhou Vocational College of Science &Technology, Wenzhou 325006, P.R. China.
Siwei
S
Sun
Department of Animal Science, Wenzhou Vocational College of Science &Technology, Wenzhou 325006, P.R. China.
Yan
Y
Zhao
Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy Agricultural Sciences, Hangzhou 310021, P.R. China.
Li
L
Chen
Zhejiang Zhuowang Agriculture Sci-Tech Limited Co., Huzhou 313014, P.R. China.
Jianliang
J
Shen
Department of Animal Science, Wenzhou Vocational College of Science &Technology, Wenzhou 325006, P.R. China.
Xiuhong
X
Li
D000818:Animals; D004372:Ducks; D020869:Gene Expression Profiling; D050356:Lipid Metabolism / Q000235:genetics*; D008099:Liver / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics
D035683:MicroRNAs; D012333:RNA, Messenger