Identification of a Fluorescent Protein from Rhacostoma Atlantica.
We have cloned a novel fluorescent protein from the jellyfish Rhacostoma atlantica. The closest known related fluorescent protein is the Phialidium yellow fluorescent protein, with only a 55% amino acid sequence identity. A somewhat unusual alanine-tyrosine-glycine amino acid sequence forms the presumed chromophore of the novel protein. The protein has an absorption peak at 466 nm and a fluorescence emission peak at 498 nm. The fluorescence quantum yield was measured to be 0.77 and the extinction coefficient is 58 200 M(-1) cm(-1) . Several mutations were identified that shift the absorption peak to about 494 nm and the emission peak to between 512 and 514 nm.
Brighter Ideas, Inc., Metuchen, NJ.
Michael R
MR
Tota
Brighter Ideas, Inc., Metuchen, NJ.
Jeanna M
JM
Allen
Institute of Fluorescence and Department of Chemistry & Biochemistry, University of Maryland Baltimore County, Baltimore, MD.
Jan O
JO
Karolin
Institute of Fluorescence and Department of Chemistry & Biochemistry, University of Maryland Baltimore County, Baltimore, MD.
Chris D
CD
Geddes
Department of Biochemistry and Molecular Biology, Rutgers University, New Brunswick, NJ. crebb@rci.rutgers.edu.
William W
WW
Ward
D000818:Animals; D003001:Cloning, Molecular; D008164:Luminescent Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D037401:Scyphozoa / Q000737:chemistry* / Q000235:genetics*
D008164:Luminescent Proteins