A novel integration system of magnetically immobilized cells and a pair of graphite plate-stainless iron mesh electrodes for the bioremediation of coking wastewater.
Magnetically immobilized cells of Comamonas sp. JB coupling with electrode reaction was developed to enhance the treatment efficiency of coking wastewater containing phenol, carbazole (CA), dibenzofuran (DBF), and dibenzothiophene (DBT). The pair of graphite plate-stainless iron mesh electrodes was chosen as the most suitable electrodes. Magnetically immobilized cells coupling with graphite plate-stainless iron mesh electrodes (coupling system) exhibited high degradation activity for all the compounds, which were significantly higher than the sum by single magnetically immobilized cells and electrode reaction at the optimal voltage. Recycling experiments demonstrated that the degradation activity of coupling system increased gradually during eight recycles, indicating that there was a coupling effect between the biodegradation and electrode reaction. Phenol hydroxylase and qPCR assays confirmed that appropriate electrical stimulation could improve phenol hydroxylase activity and promote cells growth. Toxicity assessment suggested the treatment of the coking wastewater by coupling system led to less toxicity than untreated wastewater.
School of Life Science, Liaoning Normal University, Dalian 116081, China; Liaoning Key Lab of Marine Fishery Molecular Biology, Liaoning Ocean and Fisheries Science Research Institute, Dalian 116023, China.
Bei
B
Jiang
School of Life Science, Liaoning Normal University, Dalian 116081, China.
Liang
L
Tan
School of Life Science, Liaoning Normal University, Dalian 116081, China.
Shuxiang
S
Ning
School of Life Science, Liaoning Normal University, Dalian 116081, China. Electronic address: shishengnan013@163.com.
Shengnan
S
Shi
D001572:Benzofurans / Q000737:chemistry / Q000378:metabolism; D001673:Biodegradation, Environmental; D002227:Carbazoles / Q000737:chemistry / Q000378:metabolism; D018914:Cells, Immobilized / Q000737:chemistry; D003077:Coke; D020585:Comamonas / Q000737:chemistry* / Q000378:metabolism; D004566:Electrodes; D006108:Graphite; D007501:Iron; D008280:Magnetics; D006899:Mixed Function Oxygenases / Q000737:chemistry / Q000378:metabolism; D019800:Phenol / Q000737:chemistry / Q000378:metabolism; D013876:Thiophenes / Q000737:chemistry / Q000378:metabolism; D014865:Waste Disposal, Fluid / Q000295:instrumentation / Q000379:methods*; D062065:Wastewater / Q000737:chemistry*
D001572:Benzofurans; D002227:Carbazoles; D003077:Coke; D006108:Graphite; D007501:Iron; D006899:Mixed Function Oxygenases; D019800:Phenol; D013876:Thiophenes; D062065:Wastewater; C041514:carbazole; C023614:dibenzofuran; C022336:phenol 2-monooxygenase; C016366:dibenzothiophene
Bioremediation; Coking wastewater; Electrode reaction; Magnetically immobilized cells; Nanoparticles