Peptide Regulation of Skin Fibroblast Functions during Their Aging In Vitro.
The effect peptides KE, KED, AED and AEDG on proliferation (Ki-67), regeneration and aging (CD98hc), apoptosis (caspase-3), and extracellular matrix remodeling (MMP-9) in skin fibroblasts during their aging in culture were studied by immunofluorescent confocal microscopy. All studied peptides inhibited MMP-9 synthesis that increases during aging of skin fibroblasts and enhanced the expression of Ki-67 and CD98hc that are less intensively synthesized during cell aging. Peptides AED and AEDG suppressed caspase-dependent apoptosis that increases during aging of cell cultures.
St. Petersburg Institute of Bioregulation and Gerontology, St. Petersburg, Russia. miayy@yandex.ru.|I. P. Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg, Russia. miayy@yandex.ru.|Peter the Great St. Petersburg Polytechnical University, St. Petersburg, Russia. miayy@yandex.ru.
N S
NS
Lin'kova
D. O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology, St. Petersburg, Russia.
A O
AO
Drobintseva
St. Petersburg Institute of Bioregulation and Gerontology, St. Petersburg, Russia.
O A
OA
Orlova
Peter the Great St. Petersburg Polytechnical University, St. Petersburg, Russia.
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EP
Kuznetsova
St. Petersburg Institute of Bioregulation and Gerontology, St. Petersburg, Russia.|D. O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology, St. Petersburg, Russia.
V O
VO
Polyakova
St. Petersburg Institute of Bioregulation and Gerontology, St. Petersburg, Russia.|D. O. Ott Research Institute of Obstetrics, Gynecology, and Reproductology, St. Petersburg, Russia.
I M
IM
Kvetnoy
St. Petersburg Institute of Bioregulation and Gerontology, St. Petersburg, Russia.|I. P. Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg, Russia.|I. I. Mechnikov North-Western State Medical University, St. Petersburg, Russia.
V Kh
VKh
Khavinson
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016922:Cellular Senescence; D004151:Dipeptides / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009842:Oligopeptides / Q000494:pharmacology; D017208:Rats, Wistar
D004151:Dipeptides; D009842:Oligopeptides; C572340:alanyl-glutamyl-aspartic acid; C533570:lysyl-glutamyl-aspartic acid; C505410:Casp3 protein, rat; D053148:Caspase 3; D020780:Matrix Metalloproteinase 9; C572337:Mmp9 protein, rat; C421253:alanyl-glutamyl-aspartyl-glycine
aging; cell cultures; short peptides; signal molecules; skin