Multiple-injection high-throughput gas chromatography analysis.
Multiple-injection techniques have been shown to be a simple way to perform high-throughput analysis where the entire experiment resides in a single chromatogram, simplifying the data analysis and interpretation. In this study, multiple-injection techniques are applied to gas chromatography with flame ionization detection and mass detection to significantly increase sample throughput. The unique issues of implementing a traditional "Fast" injection mode of multiple-injection techniques with gas chromatography and mass spectrometry are discussed. Stacked injections are also discussed as means to increase the throughput of longer methods where mass detection is unable to distinguish between analytes of the same mass and longer retentions are required to resolve components of interest. Multiple-injection techniques are shown to increase instrument throughput by up to 70% and to simplify data analysis, allowing hits in multiple parallel experiments to be identified easily.
Process and Analytical Chemistry, Merck Research Laboratories, Rahway, NJ, USA.
Wes
W
Schafer
Process and Analytical Chemistry, Merck Research Laboratories, Rahway, NJ, USA.
Heather
H
Wang
Process and Analytical Chemistry, Merck Research Laboratories, Rahway, NJ, USA.
Christopher J
CJ
Welch
Gas chromatography; High-throughput analysis