Cell Attachment and Viability Study of PCL Nano-fiber Modified by Cold Atmospheric Plasma.
The field of tissue engineering is an emerging discipline which applies the basic principles of life sciences and engineering to repair and restore living tissues and organs. The purpose of this study was to investigate the effect of cold and non-thermal plasma surface modification of poly (ϵ-caprolactone) (PCL) scaffolds on fibroblast cell behavior. Nano-fiber PCL was fabricated through electrospinning technique, and some fibers were then treated by cold and non-thermal plasma. The cell-biomaterial interactions were studied by culturing the fibroblast cells on nano-fiber PCL. Scaffold biocompatibility test was assessed using an inverted microscope. The growth and proliferation of fibroblast cells on nano-fiber PCL were analyzed by MTT viability assay. Cellular attachment on the nano-fiber and their morphology were evaluated using scanning electron microscope. The result of cell culture showed that nano-fiber could support the cellular growth and proliferation by developing three-dimensional topography. The present study demonstrated that the nano-fiber surface modification with cold plasma sharply enhanced the fibroblast cell attachment. Thus, cold plasma surface modification greatly raised the bioactivity of scaffolds.
Department of Pilot Biotechnology, Pasteur Institute of Iran, Tehran, Iran.
Seyed Mohammad
SM
Atyabi
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Fereshteh
F
Sharifi
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran. shi_irani@yahoo.com.
Shiva
S
Irani
Department of Biomaterial, Iran Polymer and Petrochemical Institute, Tehran, Iran.
Mojgan
M
Zandi
Department of Biomaterial, Iran Polymer and Petrochemical Institute, Tehran, Iran.
Houri
H
Mivehchi
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Zahra
Z
Nagheh
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D051379:Mice; D057139:Nanofibers / Q000737:chemistry*; D058626:Plasma Gases / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry* / Q000494:pharmacology*; D017155:Wettability
D001672:Biocompatible Materials; D058626:Plasma Gases; D011091:Polyesters; C016240:polycaprolactone
Cold plasma atmospheric; Nano-fiber; Surface modification; Tissue engineering