Transcriptomic Signature of the CD24hiCD38hiTransitional B Cells Associated With an Immunoregulatory Phenotype in Renal Transplant Recipients.
The role of B cells after transplant regarding allograft rejection or tolerance has become a topic of major interest. Recently, in renal transplant recipients, a B cell signature characterized by the overexpression of CD19+CD38hiCD24hitransitional B cells has been observed in operationally tolerant patients and in belatacept-treated patients with significantly lower incidence of donor-specific antibodies. The phenotypic and functional characterization of these transitional B cells is far from exhaustive. We present the first transcriptomic and phenotypic analysis associated with this cell phenotype. Three populations were studied and compared: (i) transitional CD24hiCD38hi, (ii) CD24+CD38-, and (iii) CD24intCD38intB cells. Transcriptome bioinformatic analysis revealed a particular signature for the CD24hiCD38hipopulation. Phenotypic analysis showed that CD24hiCD38hitransitional B cells also expressed CD9, CD10, CD1b and inducible T cell costimulator ligand (ICOS-L) markers. In addition, we found enrichment of IL-10+cells among CD24hiCD38hicells expressing ICOS-L and CD1b, the latter showing regulatory properties. Renal transplant recipients treated with belatacept exhibited significant expression of CD1b. Our results show that transitional CD24hiCD38hiB cells exhibit a distinct and specific profile, and this could be helpful for understanding of immune-regulatory mechanisms and immune monitoring in the field of organ transplant and autoimmune disease.
Université Paris-Est Créteil, Faculté de Médecine, Créteil, France.|Institut Mondor de Recherche Biomédicale (IMRB), INSERM U955, Equipe 21, Créteil, France.
J
J
Bigot
Université Paris-Est Créteil, Faculté de Médecine, Créteil, France.|Institut Mondor de Recherche Biomédicale (IMRB), INSERM U955, Equipe 21, Créteil, France.|AP-HP, Groupe Hospitalier Henri-Mondor Albert-Chenevier, CIC-BT-504, Créteil, France.
C
C
Pilon
Université Paris-Est Créteil, Faculté de Médecine, Créteil, France.|Institut Mondor de Recherche Biomédicale (IMRB), INSERM U955, Equipe 21, Créteil, France.|AP-HP, Groupe Hospitalier Henri-Mondor Albert-Chenevier, Service de Néphrologie, Créteil, France.
M
M
Matignon
Université Paris-Est Créteil, Faculté de Médecine, Créteil, France.|Institut Mondor de Recherche Biomédicale (IMRB), INSERM U955, Equipe 21, Créteil, France.
C
C
Grondin
Université Paris-Est Créteil, Faculté de Médecine, Créteil, France.|Institut Mondor de Recherche Biomédicale (IMRB), INSERM U955, Equipe 21, Créteil, France.|AP-HP, Groupe Hospitalier Henri-Mondor Albert-Chenevier, Service de Néphrologie, Créteil, France.
C
C
Leibler
Université Paris-Est Créteil, Faculté de Médecine, Créteil, France.|Institut Mondor de Recherche Biomédicale (IMRB), INSERM U955, Equipe 5, Créteil, France.|AP-HP, Groupe Hospitalier Henri-Mondor Albert-Chenevier, Service Hospitalier, Créteil, France.
A
A
Aissat
Université Paris-Est Créteil, Faculté de Médecine, Créteil, France.|Institut Mondor de Recherche Biomédicale (IMRB), INSERM U955, Equipe 2, Créteil, France.|Etablissement Français du Sang, Ile-de-France Mondor, Créteil, France.
F
F
Pirenne
Université Paris-Est Créteil, Faculté de Médecine, Créteil, France.|Institut Mondor de Recherche Biomédicale (IMRB), INSERM U955, Equipe 21, Créteil, France.|AP-HP, Groupe Hospitalier Henri-Mondor Albert-Chenevier, CIC-BT-504, Créteil, France.
J L
JL
Cohen
Université Paris-Est Créteil, Faculté de Médecine, Créteil, France.|Institut Mondor de Recherche Biomédicale (IMRB), INSERM U955, Equipe 21, Créteil, France.|AP-HP, Groupe Hospitalier Henri-Mondor Albert-Chenevier, CIC-BT-504, Créteil, France.|AP-HP, Groupe Hospitalier Henri-Mondor Albert-Chenevier, Service de Néphrologie, Créteil, France.
P
P
Grimbert
D016175:B-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D015415:Biomarkers / Q000378:metabolism*; D020869:Gene Expression Profiling; D006801:Humans; D007676:Kidney Failure, Chronic / Q000235:genetics* / Q000276:immunology / Q000601:surgery; D016030:Kidney Transplantation; D010641:Phenotype; D011379:Prognosis; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D059467:Transcriptome; D066027:Transplant Recipients
D015415:Biomarkers
B cell biology; biomarker; fusion proteins and monoclonal antibodies: costimulation molecule specific; immunobiology; immunosuppressant; kidney transplantation/nephrology; translational research/science