Comparative proteomic analysis reveals intracellular targets for bacillomycin L to induce Rhizoctonia solani Kühn hyphal cell death.
Bacillomycin L, a natural iturinic lipopeptide produced by Bacillus amyloliquefaciens, is characterized by strong antifungal activity against a variety of agronomically important filamentous fungi including Rhizoctonia solani Kühn. To further understand its antifungal actions, proteomes were comparatively studied within R. solani hyphal cells treated with or without bacillomycin L. The results show that 39 proteins were alternatively expressed within cells in response to this lipopeptide, which are involved in stress response, carbohydrate, amino acid and nucleotide metabolism, cellular component organization, calcium homeostasis, protein degradation, RNA processing, gene transcription, and others, suggesting that, in addition to inducing cell membrane permeabilization, iturin exhibits antibiotic activities by targeting intracellular molecules. Based on these results, a model of action of bacillomycin L against R. solani hyphal cells was proposed. Our study provides new insight into the antibiotic mechanisms of iturins.
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Bao
B
Zhang
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; Beijing Laboratory for Food Quality and Safety, China Agricultural University, Beijing 100083, China.
Yuxuan
Y
Qin
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Yuzhu
Y
Han
Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Chunjuan
C
Dong
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; Beijing Laboratory for Food Quality and Safety, China Agricultural University, Beijing 100083, China. Electronic address: lipinglan420@126.com.
Pinglan
P
Li
Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China. Electronic address: shangqingmao@caas.cn.
Qingmao
Q
Shang
D000069977:Bacillus amyloliquefaciens / Q000737:chemistry; D002462:Cell Membrane / Q000187:drug effects; D002463:Cell Membrane Permeability / Q000187:drug effects; D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D005659:Fungicides, Industrial / Q000302:isolation & purification / Q000494:pharmacology*; D015870:Gene Expression; D020869:Gene Expression Profiling; D063990:Gene Ontology; D025301:Hyphae / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000187:drug effects / Q000235:genetics; D050296:Microbial Viability / Q000187:drug effects; D058977:Molecular Sequence Annotation; D010456:Peptides, Cyclic / Q000302:isolation & purification / Q000494:pharmacology; D020543:Proteome / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D012232:Rhizoctonia / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D005656:Fungal Proteins; D005659:Fungicides, Industrial; D010456:Peptides, Cyclic; D020543:Proteome; C015015:bacillomycin L
Antibiotics; Bacillomycin L; Iturin; Proteome; Rhizoctonia solani Kühn