In vivo simultaneous multispectral fluorescence imaging with spectral multiplexed volume holographic imaging system.
A simultaneous multispectral fluorescence imaging system incorporating multiplexed volume holographic grating (VHG) is developed to acquire multispectral images of an object in one shot. With the multiplexed VHG, the imaging system can provide the distribution and spectral characteristics of multiple fluorophores in the scene. The implementation and performance of the simultaneous multispectral imaging system are presented. Further, the system’s capability in simultaneously obtaining multispectral fluorescence measurements is demonstrated with in vivo experiments on a mouse. The demonstrated imaging system has the potential to obtain multispectral images fluorescence simultaneously.
Tsinghua University, Department of Biomedical Engineering, Haidian District, Beijing 100084, China.
Yanlu
Y
Lv
Tsinghua University, Department of Biomedical Engineering, Haidian District, Beijing 100084, China.
Jiulou
J
Zhang
Tsinghua University, Department of Biomedical Engineering, Haidian District, Beijing 100084, China.
Dong
D
Zhang
Tsinghua University, Department of Biomedical Engineering, Haidian District, Beijing 100084, China.
Wenjuan
W
Cai
National University of Singapore, Department of Biomedical Engineering, Singapore 117576, Singapore.
Nanguang
N
Chen
Tsinghua University, Department of Biomedical Engineering, Haidian District, Beijing 100084, ChinacTsinghua University, Center for Biomedical Imaging Research, Haidian District, Beijing 100084, China.
Jianwen
J
Luo
D000818:Animals; D005456:Fluorescent Dyes / Q000737:chemistry; D006696:Holography; D051379:Mice; D061848:Optical Imaging / Q000295:instrumentation* / Q000379:methods*
D005456:Fluorescent Dyes