The complete genome of a baculovirus isolated from an insect of medical interest: Lonomia obliqua (Lepidoptera: Saturniidae).
Lonomia obliqua (Lepidoptera: Saturniidae) is a species of medical importance due to the severity of reactions caused by accidental contact with the caterpillar bristles. Several natural pathogens have been identified in L. obliqua, and among them the baculovirus Lonomia obliqua multiple nucleopolyhedrovirus (LoobMNPV). The complete genome of LoobMNPV was sequenced and shown to have 120,022 bp long with 134 putative open reading frames (ORFs). Phylogenetic analysis of the LoobMNPV genome showed that it belongs to Alphabaculovirus group I (lepidopteran-infective NPV). A total of 12 unique ORFs were identified with no homologs in other sequenced baculovirus genomes. One of these, the predicted protein encoded by loob035, showed significant identity to an eukaryotic transcription terminator factor (TTF2) from the Lepidoptera Danaus plexippus, suggesting an independent acquisition through horizontal gene transfer. Homologs of cathepsin and chitinase genes, which are involved in host integument liquefaction and viral spread, were not found in this genome. As L. obliqua presents a gregarious behavior during the larvae stage the impact of this deletion might be neglectable.
Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF, Brazil.
C W
CW
Aragão-Silva
Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF, Brazil.
M S
MS
Andrade
Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF, Brazil.
D M P
DM
Ardisson-Araújo
Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF, Brazil.
J E A
JE
Fernandes
Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF, Brazil.
F S
FS
Morgado
Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF, Brazil.
S N
SN
Báo
Departamento de Entomologia, Instituto Butantan, Av. Vital Brasil, 1500, São Paulo, Brazil.
R H P
RH
Moraes
Laboratório de Biologia Molecular e Virologia, Centro de Ciências Biológicas e da Saúde (CCBS), Universidade Presbiteriana Mackenzie, São Paulo, SP, Brazil.
J L C
JL
Wolff
Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF, Brazil.
F L
FL
Melo
Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF, Brazil.
B M
BM
Ribeiro
D000818:Animals; D001483:Base Sequence; D004279:DNA, Viral / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D022761:Gene Transfer, Horizontal; D016679:Genome, Viral; D019476:Insect Proteins / Q000145:classification / Q000235:genetics; D007814:Larva / Q000378:metabolism / Q000821:virology; D009036:Moths / Q000254:growth & development / Q000378:metabolism / Q000821:virology*; D017924:Nucleopolyhedroviruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D015534:Trans-Activators / Q000145:classification / Q000235:genetics; D014157:Transcription Factors / Q000145:classification / Q000235:genetics
D004279:DNA, Viral; D019476:Insect Proteins; D015534:Trans-Activators; D014157:Transcription Factors