Antihypoxic and Neuroprotective Effects of Glial Cell-Derived Neurotrophic Factor (GDNF) in Cultures of Dissociated Hippocampal Cells under Conditions of Experimental Hypoxia.
We analyzed the effect of glial cell derived neurotrophic factor (GDNF) on changes in functional bioelectric and calcium activity in dissociated hippocampal cell cultures under conditions of modeled acute normobaric hypoxia in vitro. GDNF (1 ng/ml) partially neutralized the negative effects of hypoxia on cell survival and parameters of functional network activity. GDNF exhibited a pronounced anti-hypoxic effect.
N. I. Lobachevsky Nizhny Novgorod State University, Nizhny Novgorod, Russia. mvedunova@yandex.ru.|Nizhny Novgorod State Medical Academy, Ministry of Health of the Russian Federation, Nizhny Novgorod, Russia. mvedunova@yandex.ru.
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Vedunova
N. I. Lobachevsky Nizhny Novgorod State University, Nizhny Novgorod, Russia.|Nizhny Novgorod State Medical Academy, Ministry of Health of the Russian Federation, Nizhny Novgorod, Russia.
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Shishkina
N. I. Lobachevsky Nizhny Novgorod State University, Nizhny Novgorod, Russia.|Nizhny Novgorod State Medical Academy, Ministry of Health of the Russian Federation, Nizhny Novgorod, Russia.
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Mishchenko
N. I. Lobachevsky Nizhny Novgorod State University, Nizhny Novgorod, Russia.|Nizhny Novgorod State Medical Academy, Ministry of Health of the Russian Federation, Nizhny Novgorod, Russia.
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Mitroshina
N. I. Lobachevsky Nizhny Novgorod State University, Nizhny Novgorod, Russia.
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Astrakhanova
N. I. Lobachevsky Nizhny Novgorod State University, Nizhny Novgorod, Russia.
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Pimashkin
N. I. Lobachevsky Nizhny Novgorod State University, Nizhny Novgorod, Russia.|Nizhny Novgorod State Medical Academy, Ministry of Health of the Russian Federation, Nizhny Novgorod, Russia.
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Mukhina
D000818:Animals; D020013:Calcium Signaling; D015687:Cell Hypoxia; D002470:Cell Survival; D002478:Cells, Cultured; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000494:pharmacology / Q000502:physiology*; D006624:Hippocampus / Q000166:cytology; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000502:physiology*; D018696:Neuroprotective Agents / Q000494:pharmacology
D051100:Glial Cell Line-Derived Neurotrophic Factor; D018696:Neuroprotective Agents
dissociated hippocampal cell cultures; glial cell derived neurotrophic factor (GDNF); hypoxia; neuroprotection