Validation of the analytical method for the simultaneous determination of selected polybrominated diphenyl ethers, polychlorinated biphenyls and organochlorine pesticides in human blood serum by gas chromatography with microelectron capture detector.
BACKGROUND
Polybrominated diphenyl ethers (PBDEs) as other persistent organic pollutants like polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCPs) pose a significant hazard to human health, mainly due to interference with the endocrine system and carcinogenetic effects. Humans are exposed to these substances mainly through a food of animal origin. These pollutants are globally detected in human matrices which requires to dispose reliable and simple analytical method that would enable further studies to assess the exposure of specific human populations to these compounds.


OBJECTIVE
The purpose of this study was to modify and validate of the analytical procedure for the simultaneous determination of selected PBDEs, PCBs and OCPs in human blood serum samples.


METHODS
The analytical measurement was performed by GC-µECD following preparation of serum samples (denaturation, multiple extraction, lipid removal). Identity of the compounds was confirmed by GC-MS.


RESULTS
The method was characterised by the appropriate linearity, good repeatability (CV below 20%). The recoveries ranged from 52.9 to 125.0% depending on compound and level of fortification. The limit of quantification was set at 0.03 ng mL(-1) of serum.


CONCLUSIONS
The modified analytical method proved to be suitable for the simultaneous determination of selected PBDEs, PCBs and OCPs in human blood serum by GC-µECD with good precision.
National Institute of Public Health - National Institute of Hygiene, Department of Toxicology and Risk Assessment, Warsaw, Poland.
Małgorzata
M
Matuszak
National Institute of Public Health - National Institute of Hygiene, Department of Toxicology and Risk Assessment, Warsaw, Poland.
Maria
M
Minorczyk
National Institute of Public Health - National Institute of Hygiene, Department of Toxicology and Risk Assessment, Warsaw, Poland.
Katarzyna
K
Góralczyk
National Institute of Public Health - National Institute of Hygiene, Department of Toxicology and Risk Assessment, Warsaw, Poland.
Agnieszka
A
Hernik
National Institute of Public Health - National Institute of Hygiene, Department of Toxicology and Risk Assessment, Warsaw, Poland.
Paweł
P
Struciński
National Institute of Public Health - National Institute of Hygiene, Department of Toxicology and Risk Assessment, Warsaw, Poland.
Monika
M
Liszewska
National Institute of Public Health - National Institute of Hygiene, Department of Toxicology and Risk Assessment, Warsaw, Poland.
Katarzyna
K
Czaja
National Institute of Public Health - National Institute of Hygiene, Department of Toxicology and Risk Assessment, Warsaw, Poland.
Wojciech
W
Korcz
National Institute of Public Health - National Institute of Hygiene, Department of Toxicology and Risk Assessment, Warsaw, Poland.
Monika
M
Łyczewska
National Institute of Public Health - National Institute of Hygiene, Department of Toxicology and Risk Assessment, Warsaw, Poland.
Jan K
JK
Ludwicki
D052244:Endocrine Disruptors / Q000097:blood*; D004785:Environmental Pollutants / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry; D055768:Halogenated Diphenyl Ethers / Q000097:blood*; D006801:Humans; D010575:Pesticides / Q000097:blood*; D011075:Polybrominated Biphenyls / Q000097:blood*
D052244:Endocrine Disruptors; D004785:Environmental Pollutants; D055768:Halogenated Diphenyl Ethers; D010575:Pesticides; D011075:Polybrominated Biphenyls