Transcriptome analysis of human brain tissue identifies reduced expression of complement complex C1Q Genes in Rett syndrome.
MECP2, the gene mutated in the majority of Rett syndrome cases, is a transcriptional regulator that can activate or repress transcription. Although the transcription regulatory function of MECP2 has been known for over a decade, it remains unclear how transcriptional dysregulation leads to the neurodevelopmental disorder. Notably, little convergence was previously observed between the genes abnormally expressed in the brain of Rett syndrome mouse models and those identified in human studies.



Here we carried out a comprehensive transcriptome analysis of human brain tissue from Rett syndrome brain using both RNA-seq and microarrays.



We identified over two hundred differentially expressed genes, and identified the complement C1Q complex genes (C1QA, C1QB and C1QC) as a point of convergence between gene expression changes in human and mouse Rett syndrome brain.



The results of our study support a role for alterations in the expression level of C1Q complex genes in RTT pathogenesis.
School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, 2052, Australia.
Peijie
P
Lin
School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, 2052, Australia.
Laura
L
Nicholls
Faculty of Medicine, University of New South Wales, Sydney, NSW, 2052, Australia.
Hassan
H
Assareh
School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, 2052, Australia.
Zhiming
Z
Fang
School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, 2052, Australia.
Timothy G
TG
Amos
School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, 2052, Australia.
Richard J
RJ
Edwards
School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, 2052, Australia.
Amelia A
AA
Assareh
School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, 2052, Australia. i.voineagu@unsw.edu.au.
Irina
I
Voineagu
D000328:Adult; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D015922:Complement C1q / Q000235:genetics*; D019295:Computational Biology / Q000379:methods; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D063990:Gene Ontology; D023061:Gene Order; D006801:Humans; D051783:Methyl-CpG-Binding Protein 2 / Q000235:genetics; D051379:Mice; D008875:Middle Aged; D009154:Mutation; D010641:Phenotype; D015518:Rett Syndrome / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D015398:Signal Transduction; D059467:Transcriptome
D051783:Methyl-CpG-Binding Protein 2; D015922:Complement C1q
MECP2; Neurogenetics; Rett Syndrome; Transcriptome profiling