Characteristic and Localization of the Monitor Peptide Receptor.
The monitor peptide receptor in the small intestine was investigated. The receptor is on the intestinal mucosal cells [Biochem. J., in press]. The specific binding of the (125)I labeled monitor peptide to dispersed rat small intestinal cells was inhibited by treatment with p-toluensulfonyl-L-lysine chloromethyl ketone (TLCK), an affinity labeling reagent for trypsin. Soybean trypsin inhibitor (SBTI) did not inhibit the binding. Analysis with reduced SDS electrophoresis-autoradiography indicated that an affinity-cross-linked complex of the (125)I labeled monitor peptide and the receptor was abolished by the TLCK treatment but was not affected by the presence of SBTI. Histochemical studies found a predominatnt binding of (125)I labeled monitor peptide on cholecystokinin (CCK)-immunoreactive cells in tissue sections of the rat upper intestine. The result suggests that the monitor peptide receptor is on the surface of CCK-producing cells.
a Laboratory of Nutritonal Chemistry, Department of Food Science and Technology, Faculty of Agriculture , Kyoto University , Kyoto 606 , Japan.
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Yamanishi
a Laboratory of Nutritonal Chemistry, Department of Food Science and Technology, Faculty of Agriculture , Kyoto University , Kyoto 606 , Japan.
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Kotera
a Laboratory of Nutritonal Chemistry, Department of Food Science and Technology, Faculty of Agriculture , Kyoto University , Kyoto 606 , Japan.
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Fushiki
a Laboratory of Nutritonal Chemistry, Department of Food Science and Technology, Faculty of Agriculture , Kyoto University , Kyoto 606 , Japan.
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Soneda
a Laboratory of Nutritonal Chemistry, Department of Food Science and Technology, Faculty of Agriculture , Kyoto University , Kyoto 606 , Japan.|b Department of Anatomy , Niigata University School of Medicine , Niigata 951 , Japan.
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Iwanaga
a Laboratory of Nutritonal Chemistry, Department of Food Science and Technology, Faculty of Agriculture , Kyoto University , Kyoto 606 , Japan.
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Sugimoto