Liquid Chromatography Chip with Low-Dispersion and Low-Pressure-Drop Turn Structure Utilizing a Distribution-Controlled Pillar Array.
To realize efficient, fast separations on pillar array columns with turns, a novel turn with low-dispersion and low-pressure-drop properties was developed. This "pillar-distribution-controlled" (PDC) turn was designed as a constant-radius turn filled with octagonal pillars that were arranged to control the linear velocity of the mobile phase in the radial direction. After the pillar positions were adjusted by computational fluid dynamics analysis, 27 mm long pillar array columns with two turns were fabricated on a 20 × 20 mm(2) silicon glass plate. The PDC turns suppressed the sample dispersion to a similar extent as the previously developed tapered turn, and the pressure drop of the newly designed turn was reduced to ∼1/6 that of the tapered turn. Moreover, the C18-modified pillar array column with the PDC turns showed good bioanalytical applicability; five fluorescently labeled amino acids were separated in only 24 s at a linear velocity of 7.5 mm/s. The developed turn structure offers the advantages of longer pillar array columns with more turns for the fast analysis of complex samples.
Graduate School of Pharmaceutical Sciences, The University of Tokyo , Tokyo, Japan.
Muneki
M
Isokawa
Major in Nano-Science and Nano-Engineering, Waseda University , Tokyo, Japan.
Katsuya
K
Takatsuki
Graduate School of Pharmaceutical Sciences, The University of Tokyo , Tokyo, Japan.
Yanting
Y
Song
Major in Nano-Science and Nano-Engineering, Waseda University , Tokyo, Japan.
Kailing
K
Shih
Major in Nano-Science and Nano-Engineering, Waseda University , Tokyo, Japan.
Kanki
K
Nakanishi
Major in Nano-Science and Nano-Engineering, Waseda University , Tokyo, Japan.
Zhimin
Z
Xie
Major in Nano-Science and Nano-Engineering, Waseda University , Tokyo, Japan.
Dong Hyun
DH
Yoon
Major in Nano-Science and Nano-Engineering, Waseda University , Tokyo, Japan.
Tetsushi
T
Sekiguchi
Graduate School of Pharmaceutical Sciences, The University of Tokyo , Tokyo, Japan.
Takashi
T
Funatsu
Major in Nano-Science and Nano-Engineering, Waseda University , Tokyo, Japan.
Shuichi
S
Shoji
Graduate School of Pharmaceutical Sciences, The University of Tokyo , Tokyo, Japan.
Makoto
M
Tsunoda