Surface biofunctionalization of β-TCP blocks using aptamer 74 for bone tissue engineering.
Successful bone regeneration following oral and maxillofacial surgeries depends on efficient functionalization strategies that allow the recruitment of osteogenic progenitor cells at the tissue/implant interface. We have previously identified aptamer 74, which exhibited a binding affinity for osteogenically induced jaw periosteal cells (JPCs). In the present study, this aptamer was used for the surface biofunctionalization of β-tricalcium phosphate (β-TCP) blocks. Atomic force microscopy (AFM) measurements showed increased binding activity of aptamer 74 towards osteogenically induced JPCs compared to untreated controls. The immobilization efficiency of aptamer 74 was analyzed using the QuantiFluor ssDNA assay for 2D surfaces and by amino acid analysis for 3D β-TCP constructs. Following the successful immobilization of aptamer 74 in 2D culture wells and on 3D constructs, in vitro assays showed no significant differences in cell proliferation compared to unmodified surfaces. Interestingly, JPC mineralization was significantly higher on the 2D surfaces and higher cell adhesion was detected on the 3D constructs with immobilized aptamer. Herein, we report an established, biocompatible β-TCP matrix with surface immobilization of aptamer 74, which enhances properties such as cell adhesion on 3D constructs and mineralization on 2D surfaces. Further studies need to be performed to improve the immobilization efficiency and to develop a suitable approach for JPC mineralization growing within 3D β-TCP constructs.
Department of Oral and Maxillofacial Surgery, University Hospital Tübingen, Germany.
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N
Ardjomandi
Department of Oral and Maxillofacial Surgery, University Hospital Tübingen, Germany.
J
J
Huth
JPK Instruments AG, Berlin, Germany.
D R
DR
Stamov
Department of Oral and Maxillofacial Surgery, University Hospital Tübingen, Germany.
A
A
Henrich
Dental Practice Zahngesundheit Waiblingen, Waiblingen, Germany.
C
C
Klein
Department of Thoracic, Cardiac and Vascular Surgery, University Hospital, Tübingen, Germany.
H-P
HP
Wendel
Department of Oral and Maxillofacial Surgery, University Hospital Tübingen, Germany.
S
S
Reinert
Department of Oral and Maxillofacial Surgery, University Hospital Tübingen, Germany. Electronic address: dorothea.alexander@med.uni-tuebingen.de.
D
D
Alexander
D052157:Aptamers, Nucleotide / Q000494:pharmacology*; D001842:Bone and Bones / Q000187:drug effects / Q000502:physiology*; D002113:Calcification, Physiologic / Q000187:drug effects; D002130:Calcium Phosphates / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D018625:Microscopy, Atomic Force; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*
D052157:Aptamers, Nucleotide; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid
AFM-based force spectroscopy; Aptamer; Tissue engineering; surface biofunctionalization; β-tricalcium phosphate (β-TCP)