Differences in leukocyte differentiation molecule abundances on domestic sheep (Ovis aries) and bighorn sheep (Ovis canadensis) neutrophils identified by flow cytometry.
Although both domestic sheep (DS) and bighorn sheep (BHS) are affected by similar respiratory bacterial pathogens, experimental and field data indicate BHS are more susceptible to pneumonia. Cross-reactive monoclonal antibodies (mAbs) for use in flow cytometry (FC) are valuable reagents for interspecies comparative immune system analyses. This study describes cross-reactive mAbs that recognize leukocyte differentiation molecules (LDMs) and major histocompatibility complex antigens on DS and BHS leukocytes. Characterization of multichannel eosinophil autofluorescence in this study permitted cell-type specific gating of granulocytes for evaluating LDMs, specifically on neutrophils, by single-label FC. Evaluation of relative abundances of LDMs by flow cytometry revealed greater CD11a, CD11b, CD18 (β2 integrins) and CD 172a (SIRPα) on DS neutrophils and greater CD14 (lipopolysaccharide receptor) on BHS neutrophils. Greater CD25 (IL-2) was identified on BHS lymphocytes following Concavalin A stimulation. While DS and BHS have similar total peripheral blood leukocyte counts, BHS have proportionately more neutrophils.
Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USA; Animal Disease Research Unit, United States Department of Agriculture, Pullman, WA 99164-6630, USA. Electronic address: Maggie.Highland@ARS.USDA.GOV.
Margaret A
MA
Highland
Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USA; Animal Disease Research Unit, United States Department of Agriculture, Pullman, WA 99164-6630, USA. Electronic address: David.Schneider1@ARS.USDA.GOV.
David A
DA
Schneider
Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USA; Animal Disease Research Unit, United States Department of Agriculture, Pullman, WA 99164-6630, USA. Electronic address: Stephen.White@ARS.USDA.GOV.
Stephen N
SN
White
Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USA. Electronic address: smadsen@vetmed.wsu.edu.
Sally A
SA
Madsen-Bouterse
Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USA; Animal Disease Research Unit, United States Department of Agriculture, Pullman, WA 99164-6630, USA. Electronic address: Don.Knowles@ARS.USDA.GOV.
Donald P
DP
Knowles
Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-7040, USA. Electronic address: davisw@vetmed.wsu.edu.
William C
WC
Davis
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000032:analysis* / Q000276:immunology; D005434:Flow Cytometry; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology; D007958:Leukocyte Count; D007962:Leukocytes / Q000276:immunology* / Q000502:physiology; D018950:Lipopolysaccharide Receptors / Q000276:immunology; D008213:Lymphocyte Activation; D009504:Neutrophils / Q000276:immunology*; D012756:Sheep; D034581:Sheep, Bighorn / Q000276:immunology*; D034561:Sheep, Domestic / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D053645:Interleukin-2 Receptor alpha Subunit; D018950:Lipopolysaccharide Receptors
Autofluorescence; Bighorn sheep; Domestic sheep; Eosinophil; Flow cytometry; Leukocyte; Leukocyte differentiation molecule; Major histocompatibility complex; Monoclonal antibody; Neutrophil