Genetically encoded optical activation of DNA recombination in human cells.
We developed two tightly regulated, light-activated Cre recombinase enzymes through site-specific incorporation of two genetically-encoded photocaged amino acids in human cells. Excellent optical off to on switching of DNA recombination was achieved. Furthermore, we demonstrated precise spatial control of Cre recombinase through patterned illumination.
Department of Chemistry, University of Pittsburgh, 219 Parkman Ave, Pittsburgh, Pennsylvania 15260, USA. deiters@pitt.edu.
J
J
Luo
Medical Research Council Laboratory of Molecular Biology, Francis Crick Ave, Cambridge CB20QH, UK and Department of Chemistry and The National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Beer-Sheva, 84105, Israel.
E
E
Arbely
Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, USA.
J
J
Zhang
Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, USA.
C
C
Chou
Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, USA.
R
R
Uprety
Medical Research Council Laboratory of Molecular Biology, Francis Crick Ave, Cambridge CB20QH, UK.
J W
JW
Chin
Department of Chemistry, University of Pittsburgh, 219 Parkman Ave, Pittsburgh, Pennsylvania 15260, USA. deiters@pitt.edu.
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A
Deiters
D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics / Q000378:metabolism; D020134:Catalytic Domain; D057809:HEK293 Cells; D006801:Humans; D019426:Integrases / Q000737:chemistry / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D017020:Methanosarcina / Q000201:enzymology; D008958:Models, Molecular; D055620:Optical Phenomena; D012343:RNA, Transfer / Q000378:metabolism; D011995:Recombination, Genetic; D014443:Tyrosine / Q000378:metabolism
D014443:Tyrosine; D012343:RNA, Transfer; C045073:Cre recombinase; D019426:Integrases; D000604:Amino Acyl-tRNA Synthetases; D008239:Lysine