Identification and characterization of Dichelobacter nodosus serogroup H from ovine footrot in India.
A total of 56 foot swabs were collected from inter digital spaces of sheep with footrot lesions were screened for 16 rRNA of Dichelobacter nodosus by PCR. Out of the 56 samples, 38(67.85%) were found to be positive. All the positive samples were subjected to multiplex PCR targeting fimA gene for identification of serogroups of D. nodosus. Serogroup H was found along with serogroup B in 12 (55.26%) samples and with serogroup I in 8 (22.2%) samples. The serogroup H was identified for the first time from the Indian subcontinent. The phylogenetic analysis of the present sequence with the available serogroup H sequences of GenBank revealed to be in close association with the serotype H1.
Department of Veterinary Microbiology, College of Veterinary Science, Tirupati, AP, India. Electronic address: nagaram_vinod@yahoo.com.
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Vinod Kumar
College of Veterinary Science, Tirupati, 517 502, AP, India. Electronic address: dsreenivasulu10@gmail.com.
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Sreenivasulu
Department of Veterinary Microbiology, College of Veterinary Science, Tirupati, AP, India. Electronic address: akmicro7@gmail.com.
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Karthik
D000595:Amino Acid Sequence; D000693:Anaerobiosis; D000818:Animals; D004269:DNA, Bacterial / Q000235:genetics; D020376:Dichelobacter nodosus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D036781:Fimbriae Proteins / Q000235:genetics*; D005535:Foot Rot / Q000382:microbiology* / Q000473:pathology; D015870:Gene Expression; D007194:India; D060885:Multiplex Polymerase Chain Reaction / Q000662:veterinary; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D065288:Serogroup; D012703:Serotyping / Q000662:veterinary; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology* / Q000473:pathology
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; C063055:fimbrillin; D036781:Fimbriae Proteins
Andhra Pradesh; Dichelobacter nodosus; Footrot; India; Serogroup H; fimA gene