Comparative Transcriptome Analysis of Two Ipomoea aquatica Forsk. Cultivars Targeted To Explore Possible Mechanism of Genotype-Dependent Accumulation of Cadmium.
A low-shoot-Cd (QLQ) and a high-shoot-Cd cultivar (T308) of water spinach (Ipomoea aquatica Forsk.) were used to investigate molecular mechanism of the genotype difference in cadmium (Cd) accumulation. RNA-Seq under 9 and 72 h cadmium exposures (5 mg L(-1)) were undertaken to explore Cd induced genotype differences in molecular processes. In total, 253 747 540 clean reads were assembled into 57 524 unigenes. Among them, 6136 and 10 064 unigenes were differentially expressed in QLQ and T308, respectively. Cell wall biosynthesis genes, such as GAUT and laccase, and three Cd efflux genes (Nramp5, MATE9, and YSL7) had higher expression levels in QLQ, while the genes in sulfur and glutathione metabolism pathway, e.g., sulfate transporter and cysteine synthase, showed higher expression levels in T308. These findings would be useful for further understanding of the mechanisms related to genotype-dependent Cd accumulation and developing the molecular assisted screening and breeding of low-shoot-Cd cultivars for water spinach.
State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-Sen University , Xingang Xi Road 135, Guangzhou 510275, China.
Ying-Ying
YY
Huang
State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-Sen University , Xingang Xi Road 135, Guangzhou 510275, China.
Chuang
C
Shen
State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-Sen University , Xingang Xi Road 135, Guangzhou 510275, China.
Jing-Xin
JX
Chen
State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-Sen University , Xingang Xi Road 135, Guangzhou 510275, China.
Chun-Tao
CT
He
State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-Sen University , Xingang Xi Road 135, Guangzhou 510275, China.
Qian
Q
Zhou
State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-Sen University , Xingang Xi Road 135, Guangzhou 510275, China.
Xiao
X
Tan
State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-Sen University , Xingang Xi Road 135, Guangzhou 510275, China.
Jian-Gang
JG
Yuan
State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-Sen University , Xingang Xi Road 135, Guangzhou 510275, China.
Zhong-Yi
ZY
Yang
D002104:Cadmium / Q000032:analysis / Q000378:metabolism*; D005506:Food Contamination / Q000032:analysis; D020869:Gene Expression Profiling; D005838:Genotype; D027741:Ipomoea / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism; D059467:Transcriptome
D010940:Plant Proteins; D012989:Soil Pollutants; D002104:Cadmium
DEGs; low-shoot-Cd accumulation; molecular mechanisms; transcriptome; water spinach (Ipomoea aquatica Forsk.)