Attachment and proliferation of dental pulp stem cells on dentine treated with different regenerative endodontic protocols.
OBJECTIVE
To investigate the attachment and proliferation of dental pulp stem cells (DPSC) on dentine treated with various endodontic regeneration protocols.


METHODS
Standardized dentine samples were irrigated with sodium hypochlorite (1.5% NaOCl) and ethylenediaminetetraacetic acid (17% EDTA) and randomized into four treatment groups and two control groups. The treatment groups were treated with a clinically used concentration of triple antibiotic paste (TAP), double antibiotic paste (DAP), calcium hydroxide (Ca(OH)2) or diluted TAP in a methylcellulose system (DTAP) for 1 week. Each sample in the treatment groups was then irrigated with EDTA. The two control groups were treated with EDTA or received no treatment. Dental pulp stem cells were seeded on each dentine specimen (10 000 cells). Lactate dehydrogenase activity assays were then performed to evaluate the attached DPSC after 1 day of incubation. Water-soluble tetrazolium assays were used to determine DPSC proliferation after three additional days of incubation. Friedman's test followed by least significant difference were used for statistical analyses (α = 0.05).


RESULTS
Triple antibiotic paste and DTAP regeneration protocols, as well as EDTA-treated dentine, caused significant increases in DPSC attachment to dentine. Triple antibiotic paste, DAP and Ca(OH)2regeneration protocols caused significant reductions in DPSC proliferation on dentine. However, the DTAP regeneration protocol did not have any significant negative effects on DPSC proliferation.


CONCLUSIONS
The clinically used endodontic regeneration protocols that include the use of TAP, DAP or Ca(OH)2medicament negatively affected DPSC proliferation on dentine. However, the use of DTAP medicament during regenerative endodontic treatment may not adversely affect the proliferation of DPSC.
Department of Biomedical and Applied Sciences, Indiana University School of Dentistry, Indianapolis, IN, USA.|Department of Restorative Dental Science, King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia.
M A
MA
Alghilan
Department of Biomedical and Applied Sciences, Indiana University School of Dentistry, Indianapolis, IN, USA.
L J
LJ
Windsor
Department of Biomedical and Applied Sciences, Indiana University School of Dentistry, Indianapolis, IN, USA.
J
J
Palasuk
Department of Biomedical and Applied Sciences, Indiana University School of Dentistry, Indianapolis, IN, USA.
G H
GH
Yassen
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002126:Calcium Hydroxide / Q000494:pharmacology; D049109:Cell Proliferation; D002939:Ciprofloxacin / Q000494:pharmacology; D003782:Dental Pulp / Q000166:cytology*; D003804:Dentin / Q000187:drug effects*; D004492:Edetic Acid / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008422:Materials Testing; D008747:Methylcellulose / Q000494:pharmacology; D008795:Metronidazole / Q000494:pharmacology; D008911:Minocycline / Q000494:pharmacology; D008963:Molar; D011897:Random Allocation; D012038:Regeneration; D012388:Root Canal Irrigants / Q000494:pharmacology; D012973:Sodium Hypochlorite / Q000494:pharmacology; D013499:Surface Properties
D000900:Anti-Bacterial Agents; D012388:Root Canal Irrigants; D008795:Metronidazole; D002939:Ciprofloxacin; D008747:Methylcellulose; D004492:Edetic Acid; D012973:Sodium Hypochlorite; D007770:L-Lactate Dehydrogenase; D008911:Minocycline; D002126:Calcium Hydroxide
calcium hydroxide; dental pulp stem cells; endodontic regeneration; ethylenediaminetetraacetic acid; triple antibiotic paste