Determination of rifampicin in human plasma by high-performance liquid chromatography coupled with ultraviolet detection after automatized solid-liquid extraction.
A precise and accurate high-performance liquid chromatography (HPLC) quantification method of rifampicin in human plasma was developed and validated using ultraviolet detection after an automatized solid-phase extraction. The method was validated with respect to selectivity, extraction recovery, linearity, intra- and inter-day precision, accuracy, lower limit of quantification and stability. Chromatographic separation was performed on a Chromolith RP8column using a mixture of 0.05 m acetate buffer pH 5.7-acetonitrile (35:65, v/v) as mobile phase. The compounds were detected at a wavelength of 335 nm with a lower limit of quantification of 0.05 mg/L in human plasma. Retention times for rifampicin and 6,7-dimethyl-2,3-di(2-pyridyl) quinoxaline used as internal standard were respectively 3.77 and 4.81 min. This robust and exact method was successfully applied in routine for therapeutic drug monitoring in patients treated with rifampicin.
AP-HP, Pharmacy Department, Saint-Louis Hospital, Paris, F-75010, France.|AP-HP, Pharmacology Department, Saint-Louis Hospital, Paris, F-75010, France.
B
B
Louveau
AP-HP, Pharmacy Department, Saint-Louis Hospital, Paris, F-75010, France.|AP-HP, Pharmacology Department, Saint-Louis Hospital, Paris, F-75010, France.
C
C
Fernandez
AP-HP, Pharmacology Department, Pitie-Salpetriere Hospital, Paris, F-75013, France.
N
N
Zahr
AP-HP, Pharmacy Department, Saint-Louis Hospital, Paris, F-75010, France.|AP-HP, Pharmacology Department, Saint-Louis Hospital, Paris, F-75010, France.
H
H
Sauvageon-Martre
AP-HP, Pharmacology Department, Saint-Louis Hospital, Paris, F-75010, France.
P
P
Maslanka
AP-HP, Pharmacy Department, Saint-Louis Hospital, Paris, F-75010, France.
P
P
Faure
AP-HP, Pharmacology Department, Saint-Louis Hospital, Paris, F-75010, France.|Université Paris Diderot, Sorbonne Paris Cité, Laboratoire de Pathologie, UMR-S 1165 and INSERM U976, F-75010, Paris, France.
S
S
Mourah
AP-HP, Pharmacology Department, Saint-Louis Hospital, Paris, F-75010, France.
L
L
Goldwirt
D000900:Anti-Bacterial Agents / Q000097:blood* / Q000302:isolation & purification / Q000493:pharmacokinetics; D001331:Automation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D012015:Reference Standards; D015203:Reproducibility of Results; D012293:Rifampin / Q000097:blood* / Q000302:isolation & purification / Q000493:pharmacokinetics; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D000900:Anti-Bacterial Agents; D012293:Rifampin
HPLC-UV; automatized solid-liquid extraction; cost-effective; rifampicin; therapeutic drug monitoring; time-effective