Western Analysis of Histone Modifications (Aspergillus nidulans).
Western blotting allows for the specific detection of proteins and/or modifications of proteins by an antibody of interest. This protocol utilizes a crude nuclei extraction protocol forAspergillus nidulansto enrich for histones and other nuclear proteins prior to gel electrophoresis. Post translational modifications of histones may then be easily detected. After electrophoresis, the selected antibodies are used to detect and quantify levels of the modifications of interest.
Department of Genetics, University of Wisconsin - Madison, Madison, USA.
Alexandra
A
Soukup
Department of Bacteriology and Department of Medical Microbiology and Immunology, University of Wisconsin - Madison, Madison, USA.
Nancy P
NP
Keller