Molecular characterization and phylogenetic analysis of Fasciola gigantica from western Java, Indonesia.
Fasciola gigantica and aspermic (hybrid) Fasciola flukes are thought to be distributed in Southeast Asian countries. The objectives of this study were to investigate the distribution of these flukes from unidentified ruminants in western Java, Indonesia, and to determine their distribution history into the area. Sixty Fasciola flukes from western Java were identified as F. gigantica based on the nucleotide sequences of the nuclear phosphoenolpyruvate carboxykinase (pepck) and DNA polymerase delta (pold) genes. The flukes were then analyzed phylogenetically based on the nucleotide sequence of the mitochondrial NADH dehydrogenase subunit 1 (nad1) gene, together with Fasciola flukes from other Asian countries. All but one F. gigantica fluke were classified in F. gigantica haplogroup C, which mainly contains nad1 haplotypes detected in flukes from Thailand, Vietnam, and China. A population genetic analysis suggested that haplogroup C spread from Thailand to the neighboring countries including Indonesia together with domestic ruminants, such as the swamp buffalo, Bubalus bubalis. The swamp buffalo is one of the important definitive hosts of Fasciola flukes in Indonesia, and is considered to have been domesticated in the north of Thailand. The remaining one fluke displayed a novel nad1 haplotype that has never been detected in the reference countries. Therefore, the origin of the fluke could not be established. No hybrid Fasciola flukes were detected in this study, in contrast to neighboring Asian countries.
Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Japan; Department of Pathogenic Veterinary Science, United Graduate School of Veterinary Science, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Kei
K
Hayashi
Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Japan. Electronic address: madoka@iwate-u.ac.jp.
Madoka
M
Ichikawa-Seki
Balai Veteriner Subang, Jl. Terusan Garuda 33/11 Blok Werasari Dangdeur, Subang 41212, Indonesia.
Puttik
P
Allamanda
Balai Veteriner Subang, Jl. Terusan Garuda 33/11 Blok Werasari Dangdeur, Subang 41212, Indonesia.
Putut Eko
PE
Wibowo
Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Japan; Department of Pathogenic Veterinary Science, United Graduate School of Veterinary Science, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Uday Kumar
UK
Mohanta
Balai Veteriner Subang, Jl. Terusan Garuda 33/11 Blok Werasari Dangdeur, Subang 41212, Indonesia.
Sodirun
Balai Veteriner Subang, Jl. Terusan Garuda 33/11 Blok Werasari Dangdeur, Subang 41212, Indonesia; National Research Center for Protozoan Disease, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan.
Azirwan
A
Guswanto
National Research Center for Protozoan Disease, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan.
Yoshifumi
Y
Nishikawa
D000818:Animals; D001483:Base Sequence; D002020:Buffaloes / Q000469:parasitology*; D002681:China; D004258:DNA Polymerase III / Q000235:genetics; D018509:DNA, Helminth / Q000235:genetics; D005209:Fasciola / Q000145:classification* / Q000235:genetics*; D007214:Indonesia; D009245:NADH Dehydrogenase / Q000235:genetics; D019764:Phosphoenolpyruvate Carboxykinase (ATP) / Q000235:genetics; D010802:Phylogeny; D012418:Ruminants / Q000469:parasitology*; D017422:Sequence Analysis, DNA; D013785:Thailand; D014744:Vietnam
D018509:DNA, Helminth; D009245:NADH Dehydrogenase; D004258:DNA Polymerase III; D019764:Phosphoenolpyruvate Carboxykinase (ATP)
Fasciola gigantica; Indonesia; nad1; pepck; pold