c-Yes enhances tumor migration and invasion via PI3K/AKT pathway in epithelial ovarian cancer.
Overexpression of c-Yes has been noted to correlation with several human cancers. However, the effects of c-Yes on epithelial ovarian cancer (EOC) development remain unclear. The aim of this study is going to prove the effects of c-Yes and related mechanisms in proliferation, metastasis and invasion of EOC. Immunohistochemical analysis was performed in 119 human EOC samples, and the data was correlated with clinic pathologic features. Furthermore, western blot analysis is performed for c-Yes in EOC samples and cell lines to evaluate their protein levels and molecular interaction. Kaplan-Meier survival analysis shows that the strong expression of c-Yes exhibited a significant correlation with poor prognosis in human EOC (P<0.01(⁎)). Meanwhile, we found that knockdown of c-Yes by shRNA inhibited the ability of migration and invasion in EOC cells via the PI3K/AKT pathway. In a word, these results suggested that c-Yes plays an important role in migration and invasion of EOC.
Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Jiangsu Provice Key Laboratory for information and molecular Drug Target, Nantong University, Nantong, 226001, Jiangsu Province, China.
Yunfeng
Y
Jin
Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Jiangsu Provice Key Laboratory for information and molecular Drug Target, Nantong University, Nantong, 226001, Jiangsu Province, China.
Menghui
M
Huang
Institute of Nautical Medicine, Jiangsu Province Key Laboratory for Information and Molecular Drug Target, Nantong University, Nantong 226001, Jiangsu Province, China.
Yingying
Y
Wang
Department of Clinical Laboratory, Qilu Children's Hospital of Shandong University, Ji'nan 250022, Shandong, China.
Changying
C
Yi
Department of Gynecologic Oncology, Nantong University Cancer Hospital, Nantong University, Nantong 226001, Jiangsu Province, China.
Yan
Y
Deng
Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Jiangsu Provice Key Laboratory for information and molecular Drug Target, Nantong University, Nantong, 226001, Jiangsu Province, China.
Yannan
Y
Chen
Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Jiangsu Provice Key Laboratory for information and molecular Drug Target, Nantong University, Nantong, 226001, Jiangsu Province, China.
Lifei
L
Jiang
Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Jiangsu Provice Key Laboratory for information and molecular Drug Target, Nantong University, Nantong, 226001, Jiangsu Province, China.
Juan
J
Wang
Institute of Nautical Medicine, Jiangsu Province Key Laboratory for Information and Molecular Drug Target, Nantong University, Nantong 226001, Jiangsu Province, China.
Qin
Q
Shen
Department of Gynecologic Oncology, Nantong University Cancer Hospital, Nantong University, Nantong 226001, Jiangsu Province, China. Electronic address: rong64@163.com.
Rong
R
Liu
Department of Obstetrics and Gynecology, Affiliated Hospital of Nantong University, Jiangsu Provice Key Laboratory for information and molecular Drug Target, Nantong University, Nantong, 226001, Jiangsu Province, China. Electronic address: xqhzxl@163.com.
QinghuaXi
D000077216:Carcinoma, Ovarian Epithelial; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D055785:Gene Knockdown Techniques; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D019394:Ki-67 Antigen / Q000378:metabolism; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009375:Neoplasms, Glandular and Epithelial / Q000201:enzymology* / Q000473:pathology*; D010051:Ovarian Neoplasms / Q000201:enzymology* / Q000473:pathology*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D051575:Proto-Oncogene Proteins c-yes / Q000378:metabolism*; D015398:Signal Transduction
D019394:Ki-67 Antigen; D019869:Phosphatidylinositol 3-Kinases; D051575:Proto-Oncogene Proteins c-yes; D051057:Proto-Oncogene Proteins c-akt
Epithelial ovarian cancer (EOC); Invasion; Migration; c-Yes