Detection of bovine papillomavirus type 14 DNA sequences in urinary bladder tumors in cattle.
Bovine papillomavirus type 14 (BPV-14) is a novel Deltapapillomavirus (δPV) which is most closely related to BPV-1, -2, and -13, well-known members of the δPV genus. So far BPV-14 has been detected in cutaneous neoplastic lesions in cattle and in feline sarcoids. As BPV-14 may share biological and pathological properties with BPV-1, -2 and -13, it has been hypothesized that, like other δPVs, BPV-14 could be associated with bovine bladder neoplasia. In this study, 50 tumors of the urinary bladder of cattle were diagnosed. DNA was extracted from all tumor samples as well as from 25 normal bladder samples and submitted to BPV-14 L1 PCR and subsequent amplicon sequencing analysis. BPV-14 L1 DNA sequences of specific 195bp amplicons were obtained from 17 of 50 (34%) tumor DNA isolates; no BPV-14 DNA was detected from 25 normal samples. Amplicons revealed a 99% homology with the corresponding BPV-14 L1 DNA region (GenBank accession number KP276343.1). Co-infections by two or three δPV types were also seen. This study reveals the presence of BPV-14 DNA alone or in combination with other δPV DNA in bovine bladder tumors alone and suggests that BPV-14 could also be involved in bladder neoplasia as its E5 oncoprotein has the potential to induce cell proliferation. Furthermore, this is the first study to show the presence of BPV-14 in Europe, suggesting that BPV-14, like other δPVs, has a worldwide distribution.
Dipartimento di Medicina Veterinaria e delle Produzioni Animali, Università di Napoli Federico II, Settore di Malattie Infettive, via Delpino, 1-80137, Napoli, Italy. Electronic address: sante.roperto@unina.it.
Sante
S
Roperto
College of Science, Massey University, Palmerston North, 4442, New Zealand. Electronic address: j.munday@massey.ac.nz.
John S
JS
Munday
Istituto Zooprofilattico Sperimentale del Mezzogiorno, via della Salute, 2-80055, Portici (NA), Italy. Electronic address: federicacorrado@inwind.it.
Federica
F
Corrado
Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, via Bologna, 148-10154, Torino, Italy. Electronic address: mariella.goria@izsto.it.
Maria
M
Goria
Dipartimento di Biologia, Università di Napoli Federico II, Complesso Universitario di Monte Sant'Angelo, Cupa Nuova Cintia, 21-80126, Napoli, Italy. Electronic address: roperto@unina.it.
Franco
F
Roperto
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics; D052687:Deltapapillomavirus / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D005060:Europe; D016133:Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D001743:Urinary Bladder / Q000821:virology; D001749:Urinary Bladder Neoplasms / Q000662:veterinary* / Q000821:virology
D004279:DNA, Viral
Bladder tumors; Bovine Deltapapillomavirus; Bovine papillomavirus type 14 (BPV-14); Papillomavirus co-infection