Precise and rapid isotopomic analysis by (1)H-(13)C 2D NMR: Application to triacylglycerol matrices.
An optimized HSQC sequence was tested and applied to triacylglycerol matrices to determine their isotopic and metabolomic profiles. Spectral aliasing and non-uniform sampling approaches were used to decrease the experimental time and to improve the resolution, respectively. An excellent long-term repeatability of signal integrals was achieved enabling to perform isotopic measurements. Thirty-two commercial vegetable oils were analyzed by this methodology. The results show that this method can be used to classify oil samples according to their geographical and botanical origins.
EBSI team, Interdisciplinary Chemistry: Synthesis, Analysis, Modelling (CEISAM), University of Nantes-CNRS UMR 6230, 2 rue de la Houssinière, BP 92208, F-44322 Nantes cedex 3, France; Research Unit: Technologies et Valorisation Agroalimentaire (TVA), Laboratory of Metrology and Isotopic Fractionation, Faculty of Science, Saint-Joseph University, P.O. Box 11-514 Riad el Solh, Beirut 1107 2050, Lebanon.
Noelle
N
Merchak
EBSI team, Interdisciplinary Chemistry: Synthesis, Analysis, Modelling (CEISAM), University of Nantes-CNRS UMR 6230, 2 rue de la Houssinière, BP 92208, F-44322 Nantes cedex 3, France.
Virginie
V
Silvestre
EBSI team, Interdisciplinary Chemistry: Synthesis, Analysis, Modelling (CEISAM), University of Nantes-CNRS UMR 6230, 2 rue de la Houssinière, BP 92208, F-44322 Nantes cedex 3, France.
Laetitia
L
Rouger
EBSI team, Interdisciplinary Chemistry: Synthesis, Analysis, Modelling (CEISAM), University of Nantes-CNRS UMR 6230, 2 rue de la Houssinière, BP 92208, F-44322 Nantes cedex 3, France; Institut Universitaire de France, 1 rue Descartes, 75005 Paris, France.
Patrick
P
Giraudeau
Research Unit: Technologies et Valorisation Agroalimentaire (TVA), Laboratory of Metrology and Isotopic Fractionation, Faculty of Science, Saint-Joseph University, P.O. Box 11-514 Riad el Solh, Beirut 1107 2050, Lebanon.
Toufic
T
Rizk
Research Unit: Technologies et Valorisation Agroalimentaire (TVA), Laboratory of Metrology and Isotopic Fractionation, Faculty of Science, Saint-Joseph University, P.O. Box 11-514 Riad el Solh, Beirut 1107 2050, Lebanon. Electronic address: joseph.bejjani@usj.edu.lb.
Joseph
J
Bejjani
EBSI team, Interdisciplinary Chemistry: Synthesis, Analysis, Modelling (CEISAM), University of Nantes-CNRS UMR 6230, 2 rue de la Houssinière, BP 92208, F-44322 Nantes cedex 3, France.
Serge
S
Akoka
D066241:Carbon-13 Magnetic Resonance Spectroscopy / Q000191:economics / Q000379:methods*; D010938:Plant Oils / Q000032:analysis* / Q000145:classification; D013997:Time Factors; D014280:Triglycerides / Q000737:chemistry*; D014675:Vegetables / Q000737:chemistry*
D010938:Plant Oils; D014280:Triglycerides
Fast 2D NMR; HSQC; Isotopic profile; Metabolomic profile; Triacylglycerol