Blueprints for green biotech: development and application of standards for plant synthetic biology.
Synthetic biology aims to apply engineering principles to the design and modification of biological systems and to the construction of biological parts and devices. The ability to programme cells by providing new instructions written in DNA is a foundational technology of the field. Large-scale de novo DNA synthesis has accelerated synthetic biology by offering custom-made molecules at ever decreasing costs. However, for large fragments and for experiments in which libraries of DNA sequences are assembled in different combinations, assembly in the laboratory is still desirable. Biological assembly standards allow DNA parts, even those from multiple laboratories and experiments, to be assembled together using the same reagents and protocols. The adoption of such standards for plant synthetic biology has been cohesive for the plant science community, facilitating the application of genome editing technologies to plant systems and streamlining progress in large-scale, multi-laboratory bioengineering projects.
The Sainsbury Laboratory, Norwich Science Park, Norwich, Norfolk, NR4 7UH U.K. nicola.patron@tsl.ac.uk.
Nicola J
NJ
Patron
D001709:Biotechnology / Q000379:methods*; D018744:DNA, Plant / Q000138:chemical synthesis / Q000235:genetics*; D005818:Genetic Engineering; D018745:Genome, Plant; D010944:Plants / Q000235:genetics* / Q000378:metabolism; D058615:Synthetic Biology / Q000592:standards*
D018744:DNA, Plant
DNA assembly; biological standards; genome engineering; molecular cloning; plant biotechnology; synthetic biology