Preparation and characterization of cationic and amphoteric mannans from Candida albicans.
Cationic and amphoteric mannans from Candida albicans were prepared by chemical modification with (3-chloro-2-hydroxypropyl)trimethylammonium chloride (CHPTAC) and sodium chloroacetate under aqueous alkaline conditions. The optimal reaction conditions for mannan cationization were found to be 6h, 60°C, and NaOH/CHPTAC ratio of 1.0. Adjusting the molar ratio of cationization agent to anhydromannose unit, cationic and amphoteric mannans with degree of substitution ranging from 0.07 to 0.57 were obtained. Their structure was confirmed by elemental analysis as well as FTIR and NMR spectroscopies. Moderate decrease of molecular weight of both cationic and amphoteric mannans was recorded by size exclusion chromatography. With increasing level of modification, reduction of the antibody-binding capacity was observed by enzyme-linked immunosorbent assay.
Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovak Republic. Electronic address: alzbeta.cizova@savba.sk.
Alžbeta
A
Čížová
Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovak Republic. Electronic address: zuzana.nescakova@gmail.com.
Zuzana
Z
Neščáková
Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovak Republic. Electronic address: anna.malovikova@savba.sk.
Anna
A
Malovíková
Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovak Republic. Electronic address: slavomir.bystricky@savba.sk.
Slavomír
S
Bystrický
D000085:Acetates / Q000737:chemistry; D002176:Candida albicans / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008351:Mannans / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D020005:Propanols / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000737:chemistry
D000085:Acetates; D008351:Mannans; D020005:Propanols; D000644:Quaternary Ammonium Compounds; C001420:3-hydroxyphenyltrimethylammonium; C006972:chloroacetic acid; C496948:(3-chloro-2-hydroxypropyl)trimethylammonium chloride
(3-Chloro-2-hydroxypropyl)trimethylammonium chloride (PubChem CID: 18732); Amphoteric; Candida albicans; Carboxymethyl; Cationic; Mannan; Quaternization; Sodium chloroacetate (PubChem CID: 23665759)