Strawberry consumption alleviates doxorubicin-induced toxicity by suppressing oxidative stress.
Doxorubicin (Dox), one of the most used chemotherapeutic agents, is known to generate oxidative stress and block DNA synthesis, which result in severe dose-limiting toxicity. A strategy to protect against Dox toxic effects could be to use dietary antioxidants of which fruits and vegetable are a rich source. In this context, strawberry consumption is associated with the maintenance of good health and the prevention of several diseases, thanks to the antioxidant capacities of its bioactive compounds. The aim of the present study was to evaluate the protective effects of strawberry consumption against oxidative stress induced by Dox in rats. Animals were fed with strawberry enriched diet (15% of the total calories) for two months and Dox (10 mg/kg; i.p.) was injected at the end of the experimental period. Strawberry consumption significantly inhibited ROS production and oxidative damage biomarkers accumulation in plasma and liver tissue and alleviated histopathological changes in rat livers treated with Dox. The reduction of antioxidant enzyme activities was significantly mitigated after strawberry consumption. In addition, strawberry enriched diet ameliorated liver mitochondrial antioxidant levels and functionality. In conclusion, strawberry intake protects against Dox-induced toxicity, at plasma, liver and mitochondrial levels thanks to its high contents of bioactive compounds.
Dipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche (DISCO)-Sez. Biochimica, Facoltà di Medicina, Università Politecnica delle Marche, Ancona, Italy.
Francesca
F
Giampieri
Dipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche (DISCO)-Sez. Biochimica, Facoltà di Medicina, Università Politecnica delle Marche, Ancona, Italy; Escuela de Medicina Veterinaria y Zootecnia, Facultad de Ciencias de la Salud. Universidad de Las Américas, Campus Queri, Quito, Ecuador.
Jose M
JM
Alvarez-Suarez
Dipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche (DISCO)-Sez. Biochimica, Facoltà di Medicina, Università Politecnica delle Marche, Ancona, Italy.
Massimiliano
M
Gasparrini
Dipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche (DISCO)-Sez. Biochimica, Facoltà di Medicina, Università Politecnica delle Marche, Ancona, Italy; Area de Nutrición y Salud, Universidad Internacional Iberoamericana (UNINI), Campeche, Mexico.
Tamara Y
TY
Forbes-Hernandez
Dipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche (DISCO)-Sez. Biochimica, Facoltà di Medicina, Università Politecnica delle Marche, Ancona, Italy.
Sadia
S
Afrin
Dipartimento Scienze Biomediche e Sanita' Pubblica, Facoltà di Medicina, Università Politecnica delle Marche, Ancona, Italy.
Stefano
S
Bompadre
Dipartimento Scienze Biomediche e Sanita' Pubblica, Facoltà di Medicina, Università Politecnica delle Marche, Ancona, Italy.
Corrado
C
Rubini
Dipartimento Scienze Biomediche e Sanita' Pubblica, Facoltà di Medicina, Università Politecnica delle Marche, Ancona, Italy.
Antonio
A
Zizzi
Dipartimento Scienze e Ingegneria della Materia, dell'Ambiente ed Urbanistica, Università Politecnica delle Marche, Ancona, Italy.
Paola
P
Astolfi
Grupo de Investigación en Polifenoles (GIP-USAL), Faculty of Pharmacy, Salamanca University, Campus Miguel de Unamuno, E37007, Salamanca, Spain.
Celestino
C
Santos-Buelga
Grupo de Investigación en Polifenoles (GIP-USAL), Faculty of Pharmacy, Salamanca University, Campus Miguel de Unamuno, E37007, Salamanca, Spain.
Ana M
AM
González-Paramás
Departamento de Fisiologia, Instituto de Nutrición y Tecnología de los Alimentos ''José Mataix", Centro de Investigaciones Biomedicas, Universidad de Granada, Granada, Spain.
Josè L
JL
Quiles
Dipartimento di Scienze Agrarie, Alimentari e Ambientali, Università Politecnica delle Marche, Ancona, Italy.
Bruno
B
Mezzetti
Dipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche (DISCO)-Sez. Biochimica, Facoltà di Medicina, Università Politecnica delle Marche, Ancona, Italy; Centre for Nutrition & Health, Universidad Europea del Atlantico (UEA), Santander, Spain. Electronic address: m.a.battino@univpm.it.
Maurizio
M
Battino
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000633:toxicity*; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D001835:Body Weight; D004317:Doxorubicin / Q000633:toxicity*; D031985:Fragaria; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D009929:Organ Size; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar
D000903:Antibiotics, Antineoplastic; D000975:Antioxidants; D015415:Biomarkers; D004317:Doxorubicin
Bioactive compounds; Doxorubicin; Mitochondrial functionality; Oxidative stress; Strawberry