Effect ofHelicobacter pylorionNFKB1,p38αandTNF-αmRNA expression levels in human gastric mucosa.
Helicobacter pyloriinfects ~50% of the world population, causing chronic gastritis and other forms of cellular damage. The present study assessed the influence ofH. pylorion the mRNA expression levels of nuclear factor-κB1 (NFKB1),p38αand tumor necrosis factor-α (TNF-α) in human gastric mucosa in a southern Brazilian population. Human gastric tissue was collected by upper endoscopy andH. pyloridiagnosis was performed using a rapid urease test and histological analysis. Total RNA was extracted and purified for subsequent cDNA synthesis and analysis by quantitative polymerase chain reaction (qPCR). The gastric tissue samples were divided into four groups as follows: Normal, inactive chronic gastritis, active chronic gastritis and intestinal metaplasia. TheSDHAgene was classified as the most stable when compared withACTB,GAPDH,B2MandHPRT1genes, and was therefore selected as the reference gene for qPCR data normalization. TNF-α mRNA expression was significantly higher in samples that were positive forH. pyloriand with active chronic gastritis. However, no difference was detected in the mRNA expression levels ofNFKB1andp38αbetween the groups. The present study concluded that the presence ofH. pyloriis associated withTNF-αupregulation in human gastric mucosa, but had no effect onNFKB1andp38αmRNA expression levels.
Molecular Biology Laboratory, Univates, Lajeado, RS 95900-000, Brazil.
Henrique Sulzbach
HS
Sulzbach DE Oliveira
Molecular Biology Laboratory, Univates, Lajeado, RS 95900-000, Brazil; Medical School, Univates, Lajeado, RS 95900-000, Brazil.
Vanderlei
V
Biolchi
Molecular Biology Laboratory, Univates, Lajeado, RS 95900-000, Brazil.
Helouise Richardt
HR
Richardt Medeiros
Molecular Biology Laboratory, Univates, Lajeado, RS 95900-000, Brazil.
Daiane Bizerra Gandor
DB
Bizerra Gandor Jantsch
Molecular Biology Laboratory, Univates, Lajeado, RS 95900-000, Brazil; Medical School, Univates, Lajeado, RS 95900-000, Brazil.
Luciana Knabben
LK
Knabben DE Oliveira Becker Delving
Endoscopy Service, Hospital Bruno Born, Medical School, Univates, Lajeado, RS 95900-000, Brazil.
Roberto
R
Reckziegel
Biotechnology Program, Univates, Lajeado, RS 95900-000, Brazil.
Márcia Inês
MI
Goettert
Laboratory of Molecular Biology, Endocrinology and Tumors, Department of Physiology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, Porto Alegre, RS 90050-170, Brazil.
Ilma Simoni
IS
Brum
Molecular Biology Laboratory, Univates, Lajeado, RS 95900-000, Brazil; Medical School, Univates, Lajeado, RS 95900-000, Brazil.
Adriane
A
Pozzobon
Helicobacter pylori; gastritis; gene expression; inflammation; quantitative polymerase chain reaction