Light-sheet imaging of mammalian development.
Tackling modern cell and developmental biology questions requires fast 3D imaging with sub-cellular resolution over extended periods of time. Fluorescence microscopy has emerged as a powerful tool to image biological samples with high spatial and temporal resolution with molecular specificity. In particular, the highly efficient illumination and detection scheme of light-sheet fluorescence microscopy is starting to revolutionize the way we can monitor cellular and developmental processes in vivo. Here we summarize the state-of-the art of light-sheet imaging with a focus on mammalian development - from instrumentation, mounting and sample handling to data processing.
Cell Biology and Biophysics Unit, EMBL Heidelberg, Germany.
Gustavo
G
de Medeiros
Cell Biology and Biophysics Unit, EMBL Heidelberg, Germany.
Bálint
B
Balázs
Cell Biology and Biophysics Unit, EMBL Heidelberg, Germany. Electronic address: lars.hufnagel@embl.de.
Lars
L
Hufnagel
D000375:Aging / Q000502:physiology; D000818:Animals; D047108:Embryonic Development; D021621:Imaging, Three-Dimensional; D008322:Mammals / Q000196:embryology*; D008856:Microscopy, Fluorescence / Q000379:methods*
Light-sheet; Live-imaging; Microscopy; Mouse embryo