Biological evaluation of N-2-hydroxypropyl trimethyl ammonium chloride chitosan as a carrier for the delivery of live Newcastle disease vaccine.
Mucosal immune system plays a very important role in antiviral immune response. We prepared Newcastle disease viruses (NDV) encapsulated in N-2-hydroxypropyl trimethyl ammonium chloride chitosan (N-2-HACC) nanoparticles (NDV/La Sota-N-2-HACC-NPs) by an ionic cross linking method, and assessed the potential of N-2-HACC-NPs as a mucosal immune delivery carrier. The properties of the nanoparticles were determined by transmission electron microscopy, Zeta potential and particle size analysis, encapsulation efficiency and loading capacity. NDV/La Sota-N-2-HACC-NPs have regular spherical morphologies and high stability; with 303.88±49.8nm mean diameter, 45.77±0.75mV Zeta potential, 94.26±0.42% encapsulation efficiency and 54.06±0.21% loading capacity. In vitro release assay indicated that the release of NDV from NDV/La Sota-N-2-HACC-NPs is slow. The NDV/La Sota-N-2-HACC-NPs have good biological characteristics, very low toxicity and high level of safety. Additionally, specific pathogen-free chickens immunized with NDV/La Sota-N-2-HACC-NPs showed much stronger cellular, humoral and mucosal immune responses than commercial attenuated live Newcastle disease vaccine, and NDV/La Sota-N-2-HACC-NPs reached the sustainable release effect. Our study here provides a foundation for the further development of mucosal vaccines and drugs, and the N-2-HACC-NPs should be a potential drug delivery carrier with immense potential in medical applications.
School of Biological Science and Technology, University of Jinan, Jinan 250022, People's Republic of China; Key Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin 150080, People's Republic of China. Electronic address: zybin395@126.com.
Kai
K
Zhao
Key Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin 150080, People's Republic of China; Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, People's Republic of China.
Yanwei
Y
Sun
Key Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin 150080, People's Republic of China.
Gang
G
Chen
Key Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin 150080, People's Republic of China.
Guangyu
G
Rong
Key Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin 150080, People's Republic of China.
Hong
H
Kang
Key Laboratory of Chemical Engineering Process & Technology for High-efficiency Conversion, College of Chemistry and Material Sciences, Heilongjiang University, Harbin 150080, People's Republic of China.
Zheng
Z
Jin
School of Biological Science and Technology, University of Jinan, Jinan 250022, People's Republic of China; Key Laboratory of Microbiology, School of Life Science, Heilongjiang University, Harbin 150080, People's Republic of China. Electronic address: 617427207@qq.com.
Xiaohua
X
Wang
D000818:Animals; D002645:Chickens; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry / Q000633:toxicity; D004337:Drug Carriers / Q000737:chemistry* / Q000633:toxicity; D007114:Immunization; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007371:Interferon-gamma / Q000378:metabolism; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology; D053758:Nanoparticles / Q000737:chemistry; D009522:Newcastle disease virus / Q000276:immunology* / Q000502:physiology; D010316:Particle Size; D011355:Prodrugs / Q000737:chemistry / Q000378:metabolism; D012449:Safety; D019562:Viral Load / Q000276:immunology; D014765:Viral Vaccines / Q000737:chemistry* / Q000276:immunology
D004337:Drug Carriers; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D011355:Prodrugs; D014765:Viral Vaccines; C495830:chitosan-N-2-hydroxypropyl trimethyl ammonium chloride; D007371:Interferon-gamma; D048271:Chitosan
Chitosan nanoparticles; Immune efficacy; Live Newcastle disease vaccine; Mucosal immune delivery system; N-2-hydroxypropyl trimethyl ammonium chloride; Newcastle disease