[Effects of different pH conditions on ffh gene expression in Streptococcus mutans].
OBJECTIVE
This research aimed to detect the expression levels of ffh gene in Streptococcus mutans (S. mutans) UA159 under different pH conditions, analyze the effect of pH on the expression of ffh gene in S. mutans, and identify the factors regulating the ffh gene expression.


METHODS
Samples of S. mutans were collected at different growth stages (4 h, 18 h) and pH values (pH 4.0-7.0). Fluorescence quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure the relative mRNA expression and trend of the target gene ffh in S. mutans at different growth stages and pH values.


RESULTS
qRT-PCR results showed that the ffh gene expression decreased along with pH at 4 h, but the expression increased with decreasing pH at 18 h. Under the same pH conditions, the ffh gene expression was significantly different between 4 h and 18 h (P < 0.05).


CONCLUSIONS
Growth stage and pH value influenced the ffh gene expression in S. mutans.
Chen
C
Zewen
Li
L
Jing
Li
L
Kaide
Qiu
Q
Chuanbin
Qiao
Q
Yueyin
Xue
X
Jing
Li
L
Yuqing
D001426:Bacterial Proteins; D015870:Gene Expression; D015964:Gene Expression Regulation, Bacterial; D006863:Hydrogen-Ion Concentration; D013295:Streptococcus mutans
D001426:Bacterial Proteins