Identification mouse patatin-like phospholipase domain containing protein 1 as a skin-specific and membrane-associated protein.
Patatin-like phospholipase domain containing protein 1 (PNPLA1) mutations have been identified to be associated with autosomal recessive congenital ichthyosis (ARCI) in recent years. However, its molecular characters have not been achieved until now. In the current study, the full length coding cDNA sequence of mouse PNPLA1 (mPNPLA1) was identified firstly. There were several putative transmembrane domains (TMDs) in mPNPLA1 by bioinformation analysis. mPNPLA1 was further found to be expressed exclusively in the membrane fraction in mammalian cells. However, it did not colocalized with the endoplasmic reticulum (ER) or lipid droplets (LDs). Moreover, the mRNA levels of mPNPLA1 was detected to be highly expressed in the skin, while very weak or even less in other mouse tissues by quantitative PCR. In addition, based on experiments with inhibitors and inducer of protein degradation pathways, mPNPLA1 was demonstrated to be degraded by macroautophagy, but not by the proteasome. These results indicated PNPLA1 was a skin-specific and membrane-associated protein for the first time, suggesting that it may mainly play a role in the skin.
Key Laboratory of Molecular Biology, College of Bio-information, Chongqing University of Posts and Telecommunications, Chongqing 400065, PR China. Electronic address: changpa@cqupt.edu.cn.
Ping-An
PA
Chang
Key Laboratory of Molecular Biology, College of Bio-information, Chongqing University of Posts and Telecommunications, Chongqing 400065, PR China.
Li-Ping
LP
Han
Key Laboratory of Molecular Biology, College of Bio-information, Chongqing University of Posts and Telecommunications, Chongqing 400065, PR China.
Lan-Xi
LX
Sun
Key Laboratory of Molecular Biology, College of Bio-information, Chongqing University of Posts and Telecommunications, Chongqing 400065, PR China.
Fei-Fei
FF
Huang
D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D004721:Endoplasmic Reticulum / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D008049:Lipase / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D066292:Lipid Droplets / Q000378:metabolism; D008565:Membrane Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D051379:Mice; D009154:Mutation; D010740:Phospholipases / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D000072417:Protein Domains; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012867:Skin / Q000378:metabolism*; D000217:Acyltransferases
D008049:Lipase; D008565:Membrane Proteins; D010740:Phospholipases; C000613695:PNPLA1 protein, mouse; D000217:Acyltransferases
Macroautophagy; Membrane protein; Mouse; PNPLA1; Skin