Evaluation of the integrated hydrothermal carbonization-algal cultivation process for enhanced nitrogen utilization in Arthrospira platensis production.
Sustainable microalgal cultivation at commercial scale requires nitrogen recycling. This study applied hydrothermal carbonization to recover N of hot-water extracted Arthrospira platensis biomass residue into aqueous phase (AP) under different operation conditions and evaluated the N utilization, biomass yield and quality of A. platensis cultures using AP as the sole N source. With the increase of temperature at 190-210°C or reaction time of 2-3h, the N recovery rate decreased under nitrogen-repletion (+N) cultivation, while contrarily increased under nitrogen-limitation (-N) cultivation. Under +N biomass accumulation in the cultures with AP under 190°C was enhanced by 41-67% compared with that in NaNO3, and the highest protein content of 51.5%DW achieved under 200°C-2h was also 22% higher. Carbohydrate content of 71.4%DW under -N cultivation achieved under 210°C-3h was 14% higher than that in NaNO3. HTC-algal cultivation strategy under -N mode could save 60% of conventional N.
Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, PR China.
Changhong
C
Yao
Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, PR China.
Peichun
P
Wu
Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, PR China; College of Environment and Chemical Engineering, Dalian University, Dalian 116622, PR China.
Yanfei
Y
Pan
Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, PR China.
Hongbin
H
Lu
Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, PR China.
Lei
L
Chi
Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, PR China; School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, PR China.
Yingying
Y
Meng
Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, PR China.
Xupeng
X
Cao
Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, PR China. Electronic address: xuesong@dicp.ac.cn.
Song
S
Xue
School of Energy and Power Engineering, Energy and Environment International Centre, Beihang University, Beijing 100191, PR China.
Xiaoyi
X
Yang
D001431:Bacteriological Techniques; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D050260:Carbohydrate Metabolism; D002244:Carbon / Q000737:chemistry / Q000378:metabolism; D058086:Microalgae / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism*; D059027:Recycling; D053545:Spirulina / Q000254:growth & development* / Q000378:metabolism*; D013696:Temperature
D002244:Carbon; D009584:Nitrogen
Arthrospira platensis; Biomass quality; Hydrothermal carbonization; Microalgal cultivation; Nitrogen recycling