Preclinical assessment of scleral lens as a reservoir-based ocular therapeutic system.
OBJECTIVE
Bacterial keratitis is a sight threatening infection of the cornea which remains one of the most important potential complications of contact lens use. If the corneal ulcer is small, peripheral with no impending perforation present, intensive monotherapy with fluoroquinolones could be used. Therefore, a study was conducted with the objective to provide pharmacological data of the intra-ocular diffusion after administration of Ofloxacin using a scleral lens reservoir, as well as an evaluation of surface tolerability in rabbits.


METHODS
Samples of corneas, aqueous humor and vitreous were collected to measure the drug levels of Ofloxacin using High Performance Liquid Chromatography. The corneas were examined by electron microscopy scanning and the eyeballs by light polarizing microscopy in order to evaluate surface tolerability.


RESULTS
Ofloxacin levels found in the aqueous humor and cornea were higher than those previously reported. The mean Ofloxacin corneal levels exceeded the MIC (Minimum Inhibitory Concentration) for which 90% of isolates are indicated for all bacteria implicated in keratitis.


CONCLUSIONS
To our knowledge, this is the first preclinical study assessing local tolerance and intra-ocular diffusion of Ofloxacin after administration using a scleral lens reservoir.
Caen University Hospital, Department of Ophthalmology, Caen, F-14033, France. Electronic address: renaud.laballe@gmail.com.
Renaud
R
Laballe
Caen University Hospital, Department of Pharmacy, Caen, F-14033, France. Electronic address: jonathan.vigne.fr@gmail.com.
Jonathan
J
Vigne
Caen University Hospital, Department of Ophthalmology, Caen, F-14033, France. Electronic address: denion-e@chu-caen.fr.
Eric
E
Denion
Rennes University Hospital, Department of Clinical and Biological Pharmacology and Pharmacovigilance, Pharmacoepidemiology and Drug Information Centre, Rennes, F-35000, France. Electronic address: florian.lemaitre@chu-rennes.fr.
Florian
F
Lemaitre
CMAbio, IBFA, SFR 4206 ICORE, University of Caen Normandy, Caen, F-14000, France. Electronic address: didier.goux@unicaen.fr.
Didier
D
Goux
Tours University Hospital, Department of Ophthalmology, Université François Rabelais, Hôpital Bretonneau, Tours, F-37000, France. Electronic address: pisella@med.univ-tours.fr.
Pierre-Jean
PJ
Pisella
D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry; D003261:Contact Lenses; D004353:Drug Evaluation, Preclinical; D004343:Drug Implants / Q000008:administration & dosage* / Q000737:chemistry*; D066298:In Vitro Techniques; D015242:Ofloxacin / Q000008:administration & dosage* / Q000737:chemistry*; D011817:Rabbits; D012590:Sclera / Q000737:chemistry* / Q000187:drug effects; D014018:Tissue Distribution
D000900:Anti-Bacterial Agents; D004343:Drug Implants; D015242:Ofloxacin
Corneal epithelium; Drug delivery; Keratitis; Ofloxacin; Scleral lens