Ex Vivo Treatment with a Polyphenol-Enriched Cocoa Extract Ameliorates Myocardial Infarct and Postischemic Mitochondrial Injury in Normotensive and Hypertensive Rats.
Our objective was to determine the effects of a polyphenol-enriched cocoa extract (PCE) on myocardial postischemic alterations in normotensive (Wistar rats, W) and spontaneously hypertensive rats (SHR). Isolated hearts were submitted to 110 min of perfusion or 20 min stabilization, 30 min global ischemia, and 60 min reperfusion (R). Other hearts were treated with PCE at the onset of R. Infarct size, the reduced glutathione (GSH), and the expression of phospho-Akt, P-GSK-3β, and P-eNOS were assessed. In isolated mitochondria, the Ca(2+)-mediated response of mitochondrial permeability transition pore (mPTP), membrane potential (Δψm), and superoxide production were determined. PCE decreased infarct size, partly preserved GSH, increased the P-Akt, P-GSK-3β, and P-eNOS contents, improved mPTP response to Ca(2+), decreased the superoxide production, and restored Δψm. These data show that PCE decreases the cardiac postischemic damage in W rats and SHR and suggest that Akt/GSK-3β/eNOS dependent pathways are involved.
Centro de Investigaciones Cardiovasculares CCT-CONICET, Universidad Nacional de La Plata , 60 y 120, 1900 La Plata, Argentina.
Luisa F
LF
González Arbeláez
Centro de Investigaciones Cardiovasculares CCT-CONICET, Universidad Nacional de La Plata , 60 y 120, 1900 La Plata, Argentina.
Alejandro
A
Ciocci Pardo
Centro de Investigaciones Cardiovasculares CCT-CONICET, Universidad Nacional de La Plata , 60 y 120, 1900 La Plata, Argentina.
Juliana C
JC
Fantinelli
Centro de Investigaciones Cardiovasculares CCT-CONICET, Universidad Nacional de La Plata , 60 y 120, 1900 La Plata, Argentina.
Claudia
C
Caldiz
Departament de Farmacologia, Facultat de Farmàcia, Universitat de València , 46010 València, Spain.
José Luis
JL
Ríos
Facultad de Ciencias Médicas, Universidad Nacional de La Plata, CIC , 1900 La Plata, Provincia de Buenos Aires, Argentina.
Guillermo R
GR
Schinella
Centro de Investigaciones Cardiovasculares CCT-CONICET, Universidad Nacional de La Plata , 60 y 120, 1900 La Plata, Argentina.
Susana M
SM
Mosca
D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D002316:Cardiotonic Agents / Q000008:administration & dosage*; D003041:Coca / Q000737:chemistry*; D005978:Glutathione / Q000378:metabolism; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism; D006321:Heart / Q000187:drug effects / Q000503:physiopathology; D006801:Humans; D006973:Hypertension / Q000188:drug therapy* / Q000209:etiology / Q000503:physiopathology; D066298:In Vitro Techniques; D007511:Ischemia / Q000150:complications*; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D033681:Mitochondrial Membrane Transport Proteins / Q000187:drug effects / Q000378:metabolism; D000083162:Mitochondrial Permeability Transition Pore; D009203:Myocardial Infarction / Q000150:complications*; D009206:Myocardium / Q000378:metabolism; D010936:Plant Extracts / Q000008:administration & dosage*; D059808:Polyphenols / Q000008:administration & dosage*; D051381:Rats; D011918:Rats, Inbred SHR; D017208:Rats, Wistar; D013481:Superoxides / Q000378:metabolism
D002316:Cardiotonic Agents; D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D010936:Plant Extracts; D059808:Polyphenols; D013481:Superoxides; D038362:Glycogen Synthase Kinase 3; D005978:Glutathione
SHR; Wistar; infarct size; mitochondria; polyphenols