Association of XRCC1 Trp194 allele with risk of breast cancer, and Ki67 protein status in breast tumor tissues.
OBJECTIVE
To evaluate the role of this polymorphism as a risk factor for breast cancer in Kurdish patients and to investigate the possible association between Arg194Trp x-ray repair cross-complementing group 1 (XRCC1) gene polymorphisms with clinical and histopathological outcomes of patients with breast cancer.


METHODS
A total of 100 breast cancer patients and 200 cancer-free controls in Kurdish population of Kurdistan state admitted to Tohid Hospital, Sanandaj, Kurdistan, Iran between January 2012 and May 2015 were enrolled in this cross-sectional study. Tissue expression of estrogen receptor (ER), progesteron receptor (PR), human epidermal growth factor receptor 2 (Her2/neu), and Ki67 were evaluated by immunohistochemistry (IHC). The Arg194Trp genotypes were determined by polymerase chain reaction- restriction fragment length polymorphism method.


RESULTS
Our data showed that the risk for breast cancer increased significantly among the Trp variant of XRCC1. Statistically significant association was found between codon 194 polymorphisms and tissue expression of Ki67.


CONCLUSIONS
The Trp allele of codon 194 XRCC1 is a potential risk factor for breast cancer in Kurdish ethnicity. Furthermore, effect of this polymorphism on clinical and histological features of breast cancer was significant.
Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran. E-mail. rsarbast@yahoo.com.
Chiya
C
Jalali
Bayazid
B
Ghaderi
Sabrieh
S
Amini
Mohammad
M
Abdi
Daem
D
Roshani
D000328:Adult; D000483:Alleles; D001943:Breast Neoplasms / Q000208:ethnology / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D003430:Cross-Sectional Studies; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D007492:Iran; D019394:Ki-67 Antigen; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D014364:Tryptophan / Q000235:genetics*; D000076105:X-ray Repair Cross Complementing Protein 1
D004268:DNA-Binding Proteins; D019394:Ki-67 Antigen; D000076105:X-ray Repair Cross Complementing Protein 1; C000619371:XRCC1 protein, human; D014364:Tryptophan