Proteomic and peptidomic differences and similarities between four muscle types from New Zealand raised Angus steers.
Four muscles from New Zealand-raised Angus steers were evaluated (musculus semitendinosus, m. longissimus thoracis et lumborum, m. psoas major and m. infraspinatus) to test their differences and common features in protein and peptide abundances. The ultimate goal of such a comparison is to match muscle types to products with targeted properties. Protein profiling based on two-dimensional electrophoresis showed that the overall profiles were similar, but, between muscle types, significant (p<0.05) intensity differences were observed in twenty four protein spots. Profiling of endogenous peptides allowed characterisation of 346 peptides. Quantitative analysis showed a clear distinction between the muscle types. Forty-four peptides were identified that showed a statistically significant (p<0.05) and substantial (>2-fold change) difference between at least two muscle types. These analyses demonstrate substantial similarities between these four muscle types, but also clear distinctions in their profiles; specifically a 25% difference between at least two muscles at the peptidomic level, and a 14% difference at the proteomic level.
Proteins & Biomaterials, AgResearch Lincoln Research Centre, Christchurch, New Zealand; Biomolecular Interaction Centre, University of Canterbury, Christchurch, New Zealand. Electronic address: stefan.clerens@agresearch.co.nz.
Stefan
S
Clerens
Proteins & Biomaterials, AgResearch Lincoln Research Centre, Christchurch, New Zealand.
Ancy
A
Thomas
Proteins & Biomaterials, AgResearch Lincoln Research Centre, Christchurch, New Zealand.
Jessica
J
Gathercole
Proteins & Biomaterials, AgResearch Lincoln Research Centre, Christchurch, New Zealand.
Jeffrey E
JE
Plowman
Proteins & Biomaterials, AgResearch Lincoln Research Centre, Christchurch, New Zealand.
Tzer-Yang
TY
Yu
Proteins & Biomaterials, AgResearch Lincoln Research Centre, Christchurch, New Zealand.
Anita J
AJ
Grosvenor
Proteins & Biomaterials, AgResearch Lincoln Research Centre, Christchurch, New Zealand.
Stephen R
SR
Haines
Food Assurance & Meat Quality, AgResearch Ruakura Research Centre, Hamilton, New Zealand.
Peter
P
Dobbie
Food Assurance & Meat Quality, AgResearch Ruakura Research Centre, Hamilton, New Zealand.
Kevin
K
Taukiri
Food Assurance & Meat Quality, AgResearch Ruakura Research Centre, Hamilton, New Zealand.
Katja
K
Rosenvold
Proteins & Biomaterials, AgResearch Lincoln Research Centre, Christchurch, New Zealand; Riddet Institute at Massey University, Palmerston North, New Zealand; Biomolecular Interaction Centre, University of Canterbury, Christchurch, New Zealand.
Jolon M
JM
Dyer
Proteins & Biomaterials, AgResearch Lincoln Research Centre, Christchurch, New Zealand.
Santanu
S
Deb-Choudhury
D000818:Animals; D001823:Body Composition; D002417:Cattle; D002853:Chromatography, Liquid; D008297:Male; D009124:Muscle Proteins / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry*; D009520:New Zealand; D057786:Peptidomimetics; D020543:Proteome / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
D009124:Muscle Proteins; D057786:Peptidomimetics; D020543:Proteome
Beef; Muscle; Peptidomics; Proteomics