The influence of manganese treatment on the distribution of metal elements in rats and the protection by sodium para-amino salicylic acid.
Manganese (Mn) overexposure induced neurological damages, which could be potentially protected by sodium para-aminosalicylic acid (PAS-Na). In this study, we systematically detected the changes of divalent metal elements in most of the organs and analyzed the distribution of the metals in Mn-exposed rats and the protection by PAS-Na. Sprague Dawley (SD) rats received intraperitoneal injections of 15mg/kg MnCl2·4H2O (5d/week for 3 weeks), followed by subcutaneous (back) injections of PAS-Na (100 and 200mg/kg, everyday for 5 weeks). The concentrations of Mn and other metal elements [Iron (Fe), Copper (Cu), Zinc (Zn), Magnesium (Mg), Calcium (Ca)] in major organs (liver, spleen, kidney, thighbone and iliac bone, cerebral cortex, hippocampus and testes) and blood by Inductively Coupled Plasma-Atomic Emission Spectrometry (ICP-AES). The results showed that Mn overexposure significantly increased Mn in most organs, Fe and Zn in liver, Fe and Mg in blood; however decreased Fe, Cu, Zn, Mg and Ca in cortex, Cu and Zn in kidney, Cu and Mg in iliac bone, and Zn in blood. In contrast, PAS-Na treatment restored most changes particularly in cortex. In conclusion, excessive Mn exposure disturbed the balance of other metal elements but PAS-Na post-treatments could restore these alterations.
Department of Toxicology, School of Public Health, Guangxi Medical University, Nanning, Guangxi, PR China.
Zong-Xiang
ZX
Yuan
Department of Toxicology, School of Public Health, Guangxi Medical University, Nanning, Guangxi, PR China; Center for Disease Control and Prevention, Nanning, PR China.
Hai-Bin
HB
Chen
Department of Toxicology, School of Public Health, Guangxi Medical University, Nanning, Guangxi, PR China.
Shao-Jun
SJ
Li
Department of Toxicology, School of Public Health, Guangxi Medical University, Nanning, Guangxi, PR China.
Xiao-Wei
XW
Huang
Department of Toxicology, School of Public Health, Guangxi Medical University, Nanning, Guangxi, PR China.
Yu-Huan
YH
Mo
Department of Toxicology, School of Public Health, Guangxi Medical University, Nanning, Guangxi, PR China.
Yi-Ni
YN
Luo
Department of Toxicology, School of Public Health, Guangxi Medical University, Nanning, Guangxi, PR China.
Sheng-Nan
SN
He
Department of Human Anatomy, School of Basic Medicine, Guangxi Medical University, Nanning, Guangxi, PR China.
Xiang-Fa
XF
Deng
Department of Toxicology, School of Public Health, Guangxi Medical University, Nanning, Guangxi, PR China; The Key Laboratory of High-Incidence Diseases Prevention and Control, Guangxi Medical University, Nanning, PR China. Electronic address: golden_lu@hotmail.com.
Guo-Dong
GD
Lu
Department of Toxicology, School of Public Health, Guangxi Medical University, Nanning, Guangxi, PR China. Electronic address: ymjianggxmu@163.com.
Yue-Ming
YM
Jiang
D010131:Aminosalicylic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D007274:Injections, Intraperitoneal; D007279:Injections, Subcutaneous; D008297:Male; D008345:Manganese / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism* / Q000494:pharmacology*; D008670:Metals / Q000097:blood / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D008670:Metals; D008345:Manganese; D010131:Aminosalicylic Acid
Manganese; Manganism; Metal elements; Sodium para-amino salicylic acid