Wild Edible Mushrooms from Turkey as Possible Anticancer Agents on HepG2 Cells Together with Their Antioxidant and Antimicrobial Properties.
This study was designed to reveal cell growth inhibitory potential of six different edible mushrooms: Ramaria flava, Agrocybe molesta, Volvopluteus gloiocephalus, Lactarius deliciosus, Bovista plumbea, and Tricholoma terreum on HepG2 cells together with their antioxidant and antibacterial power. Methanolic extracts of V gloiocephalus and aqueous extracts of R. flava had the most potential cytotoxic effects over HepG2 cells. The best results for 2,2-diphenyl-1-picrylhydrazyl radical scavenging activities were obtained from both aqueous and methanolic extracts of R. flava. Methanolic extracts of T. terreum (IC50 = 1.62 mg/mL) and aqueous extracts of B. plumbea (IC50 = 0.49 mg/mL) showed maximum metal chelating activity. The highest reducing capacities were observed among the methanolic extracts of R. flava (EC50 = 1.65 mg/mL) and aqueous extracts of B. plumbea (EC50 = 1.71 mg/ mL). High-performance liquid chromatography analysis revealed the presence of many phenolic compounds in macrofungi; gallic acid and p-coumaric acid were the two main phenolics identified in all extracts. Antibacterial studies indicated that all six tested mushrooms showed antibacterial activity on at least three microorganisms. These results indicate that different extracts of the investigated mushrooms have considerable cytotoxic, antioxidant, and antibacterial properties and may be utilized as a promising source of therapeutics.
Department of Biology, Karamanoglu Mehmetbey University, Karaman, Turkey.
Gokhan
G
Sadi
Department of Biology, Karamanoglu Mehmetbey University, Karaman, Turkey.
Abdullah
A
Kaya
Department of Biology, Karamanoglu Mehmetbey University, Karaman, Turkey.
Hicret Asli
HA
Yalcin
Department of Biology, Karamanoglu Mehmetbey University, Karaman, Turkey.
Bugrahan
B
Emsen
Department of Biology, Karamanoglu Mehmetbey University, Karaman, Turkey.
Aytac
A
Kocabas
Biochemistry Graduate Programme, Middle East Technical University, Ankara, Turkey.
Deniz Irtem
DI
Kartal
Biochemistry Graduate Programme, Middle East Technical University, Ankara, Turkey.
Ahmet
A
Altay
D000363:Agaricales / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D001487:Basidiomycota / Q000737:chemistry*; D001688:Biological Products / Q000302:isolation & purification / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000302:isolation & purification / Q000494:pharmacology; D003373:Coumaric Acids / Q000302:isolation & purification / Q000494:pharmacology; D003603:Cytotoxins / Q000302:isolation & purification / Q000494:pharmacology; D056945:Hep G2 Cells; D006801:Humans; D010851:Picrates / Q000302:isolation & purification / Q000494:pharmacology; D011422:Propionates; D014421:Turkey
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D000975:Antioxidants; D001688:Biological Products; D001713:Biphenyl Compounds; D003373:Coumaric Acids; D003603:Cytotoxins; D010851:Picrates; D011422:Propionates; C004931:1,1-diphenyl-2-picrylhydrazyl; C495469:p-coumaric acid