[ENHANCEMENT OF AGROBACTERIAL TRANSFORMATION OF PLANTS USING PROTEIN KINASE INHIBITORS TRIFLUOPERAZINE AND GENISTEIN].
The effect of different concentrations of protein tyrosine kinase inhibitor, genistein and serine/threonine protein kinase inhibitor, trifluoperazine, on the frequency of Agrobacterium-mediated transformation of leaf explants of N. tabacum was investigated. The influence of different concentrations of trifluoperazine in the range from 10 to 300 μM was investigated. It was found that 10 μM trifluoperazine provoked the increase of the frequency of agrobacterial transformation of tobacco leaf disks on 25%. In parallel, the influence of different concentrations of genistein in the range from 10 to 100 μM was investigated. It was found 100 μM genistein provoked the increase of the frequency of agrobacterial transformation of tobacco leaf disks on 12%.
A I
AI
Yemets
V V
VV
Fedorchuk
Ya B
YB
Blume
D016960:Agrobacterium tumefaciens / Q000187:drug effects / Q000235:genetics*; D004305:Dose-Response Relationship, Drug; D019833:Genistein / Q000494:pharmacology*; D018515:Plant Leaves / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D047428:Protein Kinase Inhibitors / Q000494:pharmacology*; D014026:Nicotiana / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D014170:Transformation, Genetic / Q000187:drug effects*; D014268:Trifluoperazine / Q000494:pharmacology*
D047428:Protein Kinase Inhibitors; D014268:Trifluoperazine; D019833:Genistein