Effect of hypoxia on expression of placental trophoblast cells SATB1 and β-catenin and its correlation with the pathogenesis of preeclampsia.
OBJECTIVE
To study the effect of hypoxia on the expression of placental trophoblast cells SATB1 and β-catenin and its correlation with the pathogenesis of preeclampsia.


METHODS
Trophoblastic cell lines HRT8/SVneo were cultured, SATB1 and β-catenin expression and cell biological behavior were determined after hypoxia reoxygenation treatment; cell biological behavior and the expression of related genes were determined after the transfection of SATB1 and β-catenin siRNA; preeclampsia placenta and normal placenta tissues were collected and the expression of SATB1 and β-catenin were determined.


RESULTS
OD value, cell migration rate, mRNA contents of SATB1 and β-catenin of H/R group were significantly lower than those of Nor group, cell apoptosis rate was higher than that of Nor group and the number of invasive cells was less than that of Nor group; OD value and bcl-2 mRNA content of SATB1-siRNA group were lower than those of NC group; cell apoptosis rate as well as Bax, Caspase-3, Caspase-6 and Caspase-9 mRNA contents were higher than those of NC group; cell migration rate as well as CTSB, CTSD, MMP2 and MMP9 mRNA contents of β-catenin-siRNA group were lower than those of NC group; the number of invasive cells was less than that of NC group; the expression levels of SATB1 and β-catenin in preeclampsia placenta tissue were significantly lower than those in normal placenta tissue.


CONCLUSIONS
Hypoxia can inhibit the expression of SATB1 and β-catenin in the pathogenesis of preeclampsia, which can affect the proliferation, apoptosis, migration and invasion of cells.
Department of Clinical Laboratory, Linyi People's Hospital, LinYi 276003, China.
Qiang
Q
Wu
Department of Obstetrics, General Hospital of Laiwu Iron and Steel Company, Laiwu 271126, China.
Gang
G
Wu
Department of Proctology, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China. Electronic address: bjbjljx2011@163.com.
Jing-Xiang
JX
Li
Beta-catenin; Placental trophoblast; Preeclampsia; SATB1