Platelet Transfusion Outcome and Flow Cytometric Monocyte Phagocytic Assay (FMPA).
BACKGROUND
This study was designed to evaluate platelet transfusion outcome via flow cytometric monocyte phagocytic assay (FMPA).


METHODS
Fifteen patients with a history of multiple platelet transfusions and fifteen controls were enrolled in this study. CMFDA-labeled platelets were incubated with patients' sera and were finally incubated with monocytes in a tube and analyzed by flow cytometry. Monocytes that phagocytosed platelets were detected as a CMFDA-positive platelet population via monocyte gate. The FMPA results were compared with CCI results for the patients.


RESULTS
The FMPA result correlated with 1-hour (r = -0.885, P = 0.001) and 24-hour (r = -0.884, P = 0.001) CCI. There is a significant difference in means of FMPA results between the patients with immune platelet refractoriness (68.46 ± 10.4%), non-refractory group (37.73 ± 15.21%) and the control group (18.27 ± 2.86%).


CONCLUSIONS
Our data showed that FMPA has good results in evaluation of platelet transfusion outcome and may be useful as an indicator of platelet transfusion response.
High Institute for Research and Education in Transfusion Medicine.
Mohammad
M
Sayyadi
Blood Transfusion Research Center /High Institute for Research and Education in Transfusion Medicine, Immunohematology Department, Tehran, Iran.
Mojgan
M
Shaiegan
Blood Transfusion Research Center /High Institute for Research and Education in Transfusion Medicine, Immunohematology Department, Tehran, Iran.
Mahin
M
Nikougoftar Zarif
Hematology/Oncology department- Hematology, Oncology and Stem Cell Transplantation Research Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Mohammad
M
Vaezi
Hematology, Oncology and Stem Cell Transplantation Research Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Saeed
S
Mohammadi
D000328:Adult; D000368:Aged; D001792:Blood Platelets / Q000166:cytology*; D016022:Case-Control Studies; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006801:Humans; D007492:Iran; D015470:Leukemia, Myeloid, Acute / Q000150:complications*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology*; D017713:Platelet Transfusion; D013921:Thrombocytopenia / Q000628:therapy*; D016896:Treatment Outcome
D005452:Fluoresceins; D005456:Fluorescent Dyes; C069306:5-chloromethylfluorescein