Foci of cyclin A2 interact with actin and RhoA in mitosis.
Cyclin A2 is a key player in the regulation of the cell cycle. Its degradation in mid-mitosis depends primarily on the ubiquitin-proteasome system (UPS), while autophagy also contributes. However, a fraction of cyclin A2 persists beyond metaphase. In this work, we focus on cyclin A2-rich foci detected in mitosis by high resolution imaging and analyse their movements. We demonstrate that cyclin A2 interacts with actin and RhoA during mitosis, and that cyclin A2 depletion induces a dramatic decrease in active RhoA in mitosis. Our data suggest cyclin A2 participation in RhoA activation in late mitosis.
Institut de Génétique Moléculaire de Montpellier, CNRS, Université de Montpellier, 1919 route de Mende, 34293 Montpellier, France.
Abdelhalim
A
Loukil
Institut de Génétique Moléculaire de Montpellier, CNRS, Université de Montpellier, 1919 route de Mende, 34293 Montpellier, France.
Fanny
F
Izard
Institut de Génétique Moléculaire de Montpellier, CNRS, Université de Montpellier, 1919 route de Mende, 34293 Montpellier, France.|Dynamique des Interactions Membranaires Normales et Pathologiques, CNRS, Université de Montpellier, Pl. E. Bataillon, 34095 Montpellier Cedex 5, France.
Mariya
M
Georgieva
Dynamique des Interactions Membranaires Normales et Pathologiques, CNRS, Université de Montpellier, Pl. E. Bataillon, 34095 Montpellier Cedex 5, France.
Shaereh
S
Mashayekhan
Institut de Génétique Moléculaire de Montpellier, CNRS, Université de Montpellier, 1919 route de Mende, 34293 Montpellier, France.
Jean-Marie
JM
Blanchard
Dynamique des Interactions Membranaires Normales et Pathologiques, CNRS, Université de Montpellier, Pl. E. Bataillon, 34095 Montpellier Cedex 5, France.|Laboratoire Charles Coulomb, CNRS, Université de Montpellier, Pl. E. Bataillon, 34095 Montpellier Cedex 5, France.
Andrea
A
Parmeggiani
Institut de Génétique Moléculaire de Montpellier, CNRS, Université de Montpellier, 1919 route de Mende, 34293 Montpellier, France.
Marion
M
Peter
D000199:Actins / Q000378:metabolism*; D001343:Autophagy; D045744:Cell Line, Tumor; D056751:Cyclin A2 / Q000235:genetics / Q000378:metabolism*; D004789:Enzyme Activation; D006801:Humans; D061986:MCF-7 Cells; D008938:Mitosis; D020742:rhoA GTP-Binding Protein / Q000378:metabolism*
D000199:Actins; C503721:CCNA2 protein, human; D056751:Cyclin A2; C066737:RHOA protein, human; D020742:rhoA GTP-Binding Protein