P(VDF-TrFE)/BaTiO3 Nanoparticle Composite Films Mediate Piezoelectric Stimulation and Promote Differentiation of SH-SY5Y Neuroblastoma Cells.
Poly(vinylidene fluoride-trifluoroethylene, P(VDF-TrFE)) and P(VDF-TrFE)/barium titanate nanoparticle (BTNP) films are prepared and tested as substrates for neuronal stimulation through direct piezoelectric effect. Films are characterized in terms of surface, mechanical, and piezoelectric features before in vitro testing on SH-SY5Y cells. In particular, BTNPs significantly improve piezoelectric properties of the films (4.5-fold increased d31 ). Both kinds of films support good SH-SY5Y viability and differentiation. Ultrasound (US) stimulation is proven to elicit Ca(2+) transients and to enhance differentiation in cells grown on the piezoelectric substrates. For the first time in the literature, this study demonstrates the suitability of polymer/ceramic composite films and US for neuronal stimulation through direct piezoelectric effect.
Istituto Italiano di Tecnologia, Center for Micro-BioRobotics @SSSA, Viale Rinaldo Piaggio 34, 56025, Pontedera (Pisa), Italy.
Giada Graziana
GG
Genchi
Istituto Italiano di Tecnologia, Smart Materials, Nanophysics Department, Via Morego 30, 16163, Genova, Italy.
Luca
L
Ceseracciu
Istituto Italiano di Tecnologia, Center for Micro-BioRobotics @SSSA, Viale Rinaldo Piaggio 34, 56025, Pontedera (Pisa), Italy.|Scuola Superiore Sant'Anna, The BioRobotics Institute, Viale Rinaldo Piaggio 34, 56025, Pontedera (Pisa), Italy.
Attilio
A
Marino
CNR-IPCF, Largo Pontecorvo 3, 56127, Pisa, Italy.
Massimiliano
M
Labardi
Istituto Italiano di Tecnologia, Nanochemistry Department, Via Morego 30, 16163, Genova, Italy.
Sergio
S
Marras
Istituto Italiano di Tecnologia, Center for Micro-BioRobotics @SSSA, Viale Rinaldo Piaggio 34, 56025, Pontedera (Pisa), Italy.
Francesca
F
Pignatelli
University Hospital of Pisa, ENT Audiology and Phoniatry Unit, Via Paradisa 3, 56124, Pisa, Italy.
Luca
L
Bruschini
Istituto Italiano di Tecnologia, Center for Micro-BioRobotics @SSSA, Viale Rinaldo Piaggio 34, 56025, Pontedera (Pisa), Italy.
Virgilio
V
Mattoli
Istituto Italiano di Tecnologia, Center for Micro-BioRobotics @SSSA, Viale Rinaldo Piaggio 34, 56025, Pontedera (Pisa), Italy.|Politecnico di Torino, Department of Mechanical and Aerospace Engineering, Corso Duca degli Abruzzi 24, 10129, Torino, Italy.
Gianni
G
Ciofani
D017609:Barium Compounds / Q000737:chemistry*; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002470:Cell Survival; D004558:Electric Stimulation / Q000379:methods; D006801:Humans; D011145:Polyvinyls / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*
D017609:Barium Compounds; D011145:Polyvinyls; C024547:barium titanate(IV); C073666:vinylidene fluoride-trifluoroethylene copolymer; D014025:Titanium
BaTiO3 nanoparticles; P(VDF-TrFE); neurons; piezoelectric stimulation