Oleuropein ameliorates arsenic induced oxidative stress in mice.
The objective of this study is to investigate the potential preventive effect of oleuropein in an experimental arsenic toxicity in mice. For this purpose, mice were exposed to 5mg/kg/day sodium arsenite (NaAsO2) in drinking water and treated with 30mg/kg/day oleuropein for 15 days. At the end of the experiment, animals were sacrificed and selected organs were processed for biochemical and histopahtological investigations. Blood, liver, kidney and brain malondialdehyde (MDA) and nitric oxide (NO) levels were determined by colorimetric methods. Protein carbonyl content is measured by a commercial kit. Liver morphology and immunoreactivity for inducible NOS (iNOS) and endothelial NOS (eNOS) was evaluated microscopically. Level of NO was determined to decrease in blood and tissues whereas MDA increased in arsenic given mice. Tissue protein carbonyl content also increased in this group. Immunoreactivity for iNOS and eNOS was noted to increase with arsenic treatment. Oleuropein treatment had significant effects in normalizing the MDA and NO levels as well as protein carbonyl content. Immunohistochemical staining also showed reduction of the expression of iNOS and eNOS in liver. The results indicate that oleuropein ameliorates oxidative tissue damage by scavenging free radicals.
Department of Biochemistry, Faculty of Veterinary Medicine, Kafkas University, 36100 Kars, Turkey. Electronic address: metinogun@hotmail.com.
Metin
M
Ogun
Department of Biochemistry, Faculty of Medicine, Kafkas University, 36100 Kars, Turkey.
Ayla
A
Ozcan
Department of Pathology, Faculty of Veterinary Medicine, Balıkesir University, 36100 Balıkesir, Turkey.
Musa
M
Karaman
Department of Biochemistry, Faculty of Veterinary Medicine, Kafkas University, 36100 Kars, Turkey.
Oguz
O
Merhan
Department of Pathology, Faculty of Veterinary Medicine, Kafkas University, 36100 Kars, Turkey.
Hasan
H
Ozen
Department of Biochemistry, Faculty of Veterinary Medicine, Kafkas University, 36100 Kars, Turkey.
Abdulsamed
A
Kukurt
Department of Biochemistry, Faculty of Medicine, Kafkas University, 36100 Kars, Turkey.
Mahmut
M
Karapehlivan
D000818:Animals; D018053:Arsenites / Q000008:administration & dosage / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000008:administration & dosage / Q000494:pharmacology*; D057888:Iridoid Glucosides; D039823:Iridoids / Q000008:administration & dosage / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000032:analysis / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide / Q000032:analysis / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D017670:Sodium Compounds / Q000008:administration & dosage / Q000494:pharmacology*
D018053:Arsenites; D016166:Free Radical Scavengers; D057888:Iridoid Glucosides; D039823:Iridoids; D017670:Sodium Compounds; C002769:oleuropein; D009569:Nitric Oxide; C017947:sodium arsenite; D008315:Malondialdehyde; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III
Arsenic toxicity; MDA; NO; Oleuropein; Oxidative damage; Protein carbonyl content