Overproduction of cellulase by Trichoderma reesei RUT C30 through batch-feeding of synthesized low-cost sugar mixture.
Cellulase is a prerequisite for the bioconversion of lignocellulosic biomass, but its high cost presents the biggest challenge. In this article, low-cost mixture was produced from glucose through the transglycosylation reaction catalyzed by β-glucosidase for cellulase overproduction by Trichodema reesei RUT C30. As a result, cellulase titer of 90.3FPU/mL, which was more than 10 folds of that achieved with lactose as inducer, was achieved at 144h. Meanwhile, cellulase productivity was drastically increased to 627.1FPU/L/h, at least 3-5 folds higher than previously reported by the fungal species. The crude enzyme was further tested by hydrolyzing NaOH-pretreated corn stover with 15% solid loading, and 96.6g/L glucose was released with 92.6% sugar yield at 96h and 44.8g/L ethanol was obtained.
School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116023, China. Electronic address: yh_li@mail.dlut.edu.cn.
Yonghao
Y
Li
State Key Laboratory of Microbial Metabolism & School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: cg.liu@sjtu.edu.cn.
Chenguang
C
Liu
School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116023, China; State Key Laboratory of Microbial Metabolism & School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: fwbai@sjtu.edu.cn.
Fengwu
F
Bai
State Key Laboratory of Microbial Metabolism & School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: xqzhao@sjtu.edu.cn.
Xinqing
X
Zhao
D061249:Batch Cell Culture Techniques; D018533:Biomass; D002480:Cellulase / Q000096:biosynthesis* / Q000378:metabolism; D003365:Costs and Cost Analysis; D000431:Ethanol / Q000378:metabolism; D005936:Glucans / Q000737:chemistry / Q000191:economics / Q000378:metabolism*; D005947:Glucose / Q000191:economics / Q000378:metabolism; D006031:Glycosylation; D006868:Hydrolysis; D007218:Industrial Microbiology / Q000191:economics / Q000379:methods*; D007785:Lactose / Q000378:metabolism; D018520:Plant Shoots / Q000737:chemistry / Q000378:metabolism; D012972:Sodium Hydroxide / Q000737:chemistry; D014242:Trichoderma / Q000201:enzymology* / Q000378:metabolism; D003313:Zea mays / Q000737:chemistry / Q000378:metabolism; D001617:beta-Glucosidase / Q000378:metabolism
D005936:Glucans; D000431:Ethanol; D012972:Sodium Hydroxide; D001617:beta-Glucosidase; D002480:Cellulase; D005947:Glucose; D007785:Lactose; C003328:sophorose
Cellulase production; Enzymatic hydrolysis of pretreated corn stover; Fed-batch fermentation; Trichodema reesei