Efficient cutaneous wound healing using bixin-loaded PCL nanofibers in diabetic mice.
The present work demonstrated an efficient cutaneous wound healing using Bixin-loaded polycaprolactone (PCL) nanofibers as a controlled delivery system. The influence of Bixin (Bix) content on PCL nanofiber, Bix-PCL1(2.5% w/w bix) and Bix-PCL2 (12.5% w/w bix) formation was investigated using electrical conductivity, attenuated total reflectance infrared spectroscopy, X-ray diffraction, thermal analysis, and scanning electronic microscopy. The results showed that a greater bixin concentration resulted in higher polymeric solution electrical conductivity. Moreover, higher polymeric solution electrical conductivity provides lower nanofibers in terms of average diameter than pure PCL nanofibers. In vitro release was largely governed by a diffusion-controlled mechanism. The initial Bixin release domain showed a burst release over the first 10 hours where approximately 30% and 40% of Bixin was released from Bix-PCL1 and Bix-PCL2 nanofibers, respectively. The second kinetic domain was comprised of a continuous and slow Bixin release that led to almost 100% of the Bixin being released within 14 days. The results on excisional wound model in induced diabetic mice indicated that the low concentration of Bixin released from loaded Bix-PCL nanofibers maintain the biological activity of Bixin and is efficient in accelerating the wound healing as well as in reducing the scar tissue area compared with pure PCL nanofibers. Therefore, soft material Bixin-loaded PCL nanofibers are a promising candidate for use in wound dressing. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1938-1949, 2017.
Chemistry Department, Institute of Exact Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Ana Delia
AD
Pinzón-García
Department of Physiology and Biophysics, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Puebla
P
Cassini-Vieira
Chemistry Department, Institute of Exact Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Cyntia Cabral
CC
Ribeiro
Pharmacy Department, Universidade Federal de São João del Rey, São João del Rey, Minas Gerais, Brazil.
Carlos Eduardo
CE
de Matos Jensen
Department of Physiology and Biophysics, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Luciola Silva
LS
Barcelos
Department of Restorative Dentistry, Faculty of Dentristry, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Maria Esperanza
ME
Cortes
Chemistry Department, Institute of Exact Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Ruben Dario
RD
Sinisterra
D041721:3T3-L1 Cells; D000818:Animals; D002338:Carotenoids / Q000737:chemistry / Q000494:pharmacology; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D057139:Nanofibers / Q000737:chemistry / Q000627:therapeutic use; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology; D012867:Skin / Q000293:injuries* / Q000378:metabolism / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects*
D011091:Polyesters; C016240:polycaprolactone; D002338:Carotenoids; C004588:bixin
bixin; diabetes mellitus; polycaprolactone (PCL); polymeric nanofiber; wound healing