A single use electrochemical sensor based on biomimetic nanoceria for the detection of wine antioxidants.
We report the development and characterization of a disposable single use electrochemical sensor based on the oxidase-like activity of nanoceria particles for the detection of phenolic antioxidants. The use of nanoceria in the sensor design enables oxidation of phenolic compounds, particularly those with ortho-dihydroxybenzene functionality, to their corresponding quinones at the surface of a screen printed carbon electrode. Detection is carried out by electrochemical reduction of the resulting quinone at a low applied potential of -0.1V vs the Ag/AgCl electrode. The sensor was optimized and characterized with respect to particle loading, applied potential, response time, detection limit, linear concentration range and sensitivity. The method enabled rapid detection of common phenolic antioxidants including caffeic acid, gallic acid and quercetin in the µM concentration range, and demonstrated good functionality for the analysis of antioxidant content in several wine samples. The intrinsic oxidase-like activity of nanoceria shows promise as a robust tool for sensitive and cost effective analysis of antioxidants using electrochemical detection.
International Centre of Biodynamics, 1B Intrarea Portocalelor, 060101 Bucharest, Romania.
Veronica
V
Andrei
Clarkson University, Department of Chemistry and Biomolecular Science, Potsdam, NY 13699-5810, United States.
Erica
E
Sharpe
International Centre of Biodynamics, 1B Intrarea Portocalelor, 060101 Bucharest, Romania. Electronic address: avasilescu@biodyn.ro.
Alina
A
Vasilescu
Clarkson University, Department of Chemistry and Biomolecular Science, Potsdam, NY 13699-5810, United States. Electronic address: eandrees@clarkson.edu.
Silvana
S
Andreescu
D000975:Antioxidants / Q000032:analysis*; D040761:Biomimetic Materials / Q000737:chemistry*; D032701:Biomimetics / Q000379:methods; D002109:Caffeic Acids / Q000032:analysis; D002563:Cerium / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D005707:Gallic Acid / Q000032:analysis; D057230:Limit of Detection; D010084:Oxidation-Reduction; D010636:Phenols / Q000032:analysis*; D011794:Quercetin / Q000032:analysis; D014920:Wine / Q000032:analysis*
D000975:Antioxidants; D002109:Caffeic Acids; D010636:Phenols; D002563:Cerium; C030583:ceric oxide; D005707:Gallic Acid; D011794:Quercetin; C040048:caffeic acid
Amperometry; Disposable sensors; Enzyme mimetic; Nanoceria; Oxidizible phenolics; Wine antioxidants