Ethanol Induced Brain Lipid Changes in Mice Assessed by Mass Spectrometry.
Alcohol abuse is a chronic disease characterized by the consumption of alcohol at a level that interferes with physical and mental health and causes serious and persistent changes in the brain. Lipid metabolism is of particular interest due to its high concentration in the brain. Lipids are the main component of cell membranes, are involved in cell signaling, signal transduction, and energy storage. In this study, we analyzed lipid composition of chronically ethanol exposed mouse brains. Juvenile (JUV) and adult (ADU) mice were placed on a daily limited-access ethanol intake model for 52 days. After euthanasia, brains were harvested, and total lipids were extracted from brain homogenates. Samples were analyzed using high resolution mass spectrometry and processed by multivariate and univariate statistical analysis. Significant lipid changes were observed in different classes including sphingolipids, fatty acids, lysophosphatidylcholines, and other glycerophospholipids.
Structural Biology Unit, NIDA IRP, NIH , Baltimore, Maryland 21224, United States.
Aurelie
A
Roux
Structural Biology Unit, NIDA IRP, NIH , Baltimore, Maryland 21224, United States.
Shelley N
SN
Jackson
Structural Biology Unit, NIDA IRP, NIH , Baltimore, Maryland 21224, United States.|University of Pittsburgh , Pittsburgh, Pennsylvania 15260, United States.
Ludovic
L
Muller
Ionwerks Inc. , Houston, Texas 77002, United States.
Damon
D
Barbacci
Behavioral Neuropharmacology and Neuroimaging Lab, Department of Psychology, University of Buffalo , Buffalo, New York 14260, United States.
Joseph
J
O'Rourke
Behavioral Neuropharmacology and Neuroimaging Lab, Department of Psychology, University of Buffalo , Buffalo, New York 14260, United States.
Panayotis K
PK
Thanos
Structural Biology Unit, NIDA IRP, NIH , Baltimore, Maryland 21224, United States.
Nora D
ND
Volkow
Departments of Otolaryngology, Neurobiology, Communication Sciences & Disorders, and Bioengineering, University of Pittsburgh , Pittsburgh, Pennsylvania 15260, United States.
Carey
C
Balaban
Ionwerks Inc. , Houston, Texas 77002, United States.
J Albert
JA
Schultz
Structural Biology Unit, NIDA IRP, NIH , Baltimore, Maryland 21224, United States.
Amina S
AS
Woods
D000367:Age Factors; D000818:Animals; D001921:Brain / Q000187:drug effects*; D002492:Central Nervous System Depressants / Q000494:pharmacology*; D000431:Ethanol / Q000494:pharmacology*; D050356:Lipid Metabolism / Q000187:drug effects*; D008055:Lipids / Q000032:analysis*; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D008810:Mice, Inbred C57BL
D002492:Central Nervous System Depressants; D008055:Lipids; D000431:Ethanol
alcohol; electrospray ionization; lipids; mass spectrometry; solid-phase extraction