Efficient Virus-Induced Gene Silencing in Solanum rostratum.
Solanum rostratum is a "super weed" that grows fast, is widespread, and produces the toxin solanine, which is harmful to both humans and other animals. To our knowledge, no study has focused on its molecular biology owing to the lack of available transgenic methods and sequence information for S. rostratum. Virus-induced gene silencing (VIGS) is a powerful tool for the study of gene function in plants; therefore, in the present study, we aimed to establish tobacco rattle virus (TRV)-derived VIGS in S. rostratum. The genes for phytoene desaturase (PDS) and Chlorophyll H subunit (ChlH) of magnesium protoporphyrin chelatase were cloned from S. rostratum and used as reporters of gene silencing. It was shown that high-efficiency VIGS can be achieved in the leaves, flowers, and fruit of S. rostratum. Moreover, based on our comparison of three different types of infection methods, true leaf infection was found to be more efficient than cotyledon and sprout infiltration in long-term VIGS in multiple plant organs. In conclusion, the VIGS technology and tomato genomic sequences can be used in the future to study gene function in S. rostratum.
Laboratory of Food Biotechnology, College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Qinghua Donglu Road, Haidian District, Beijing, 100083, China.
Lan-Huan
LH
Meng
Laboratory of Food Biotechnology, College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Qinghua Donglu Road, Haidian District, Beijing, 100083, China.
Rui-Heng
RH
Wang
Laboratory of Food Biotechnology, College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Qinghua Donglu Road, Haidian District, Beijing, 100083, China.
Ben-Zhong
BZ
Zhu
Laboratory of Food Biotechnology, College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Qinghua Donglu Road, Haidian District, Beijing, 100083, China.
Hong-Liang
HL
Zhu
Laboratory of Food Biotechnology, College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Qinghua Donglu Road, Haidian District, Beijing, 100083, China.
Yun-Bo
YB
Luo
Laboratory of Food Biotechnology, College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Qinghua Donglu Road, Haidian District, Beijing, 100083, China.
Da-Qi
DQ
Fu
D035264:Flowers / Q000235:genetics / Q000378:metabolism; D005638:Fruit / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D020868:Gene Silencing / Q000502:physiology; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D010942:Plant Viruses / Q000235:genetics / Q000502:physiology*; D032322:Solanum / Q000235:genetics*
D010940:Plant Proteins; D010088:Oxidoreductases; C022398:phytoene dehydrogenase