Technical Aspects and Benefits of Experimental Mouse Lung Transplantation.
In recent years, the number of lung transplantations performed as the last option for many respiratory diseases has grown considerably, both in adults and children. However, the causes for the relatively short survival of lungs compared to other organ transplants still need to be studied. Techniques have improved since the 1950s when experimental lung transplantation began, and the different animal species used now include rodents. The advantage of using these small species is that the surgical model has been expanded and standardized, and different respiratory problems can be studied. In this review we examine the different technical strategies used in experimental transplantation in rats and mice, focusing on surgical techniques and anesthesia and monitoring methods, and highlighting the major contributions of mouse lung transplantation to the field.
Instituto de Investigación Sanitaria del Hospital Universitario La Paz (IdiPAZ), Madrid, España.
Daniel
D
Ruiz-Pérez
Instituto de Investigación Sanitaria del Hospital Universitario La Paz (IdiPAZ), Madrid, España.
Carlota
C
Largo
Servicio de Neumología, Hospital Universitario La Paz, IdiPAZ, Madrid, España; Facultad de Medicina, Universidad Autónoma de Madrid, Madrid, España; CIBER de Enfermedades Respiratorias (CIBERES), España. Electronic address: fgr01m@gmail.com.
Francisco
F
García-Río
D000700:Analgesics / Q000627:therapeutic use; D000768:Anesthesia, General / Q000379:methods; D000818:Animals; D006084:Graft Rejection / Q000517:prevention & control; D016040:Lung Transplantation / Q000379:methods*; D051379:Mice / Q000601:surgery*; D008866:Microsurgery / Q000379:methods; D023421:Models, Animal; D016343:Monitoring, Intraoperative / Q000379:methods; D010149:Postoperative Pain / Q000188:drug therapy; D011182:Postoperative Care / Q000379:methods; D055031:Primary Graft Dysfunction / Q000209:etiology; D051381:Rats / Q000601:surgery*; D015427:Reperfusion Injury / Q000209:etiology; D012121:Respiration, Artificial; D013045:Species Specificity; D014019:Tissue Donors; D020858:Tissue and Organ Harvesting / Q000379:methods
D000700:Analgesics
Experimental; Lung; Modelo murino; Mouse model; Pulmón; Transplantation; Trasplante