Profiling Specific Inner Ear Cell Types Using Cell Sorting Techniques.
Studies of specific tissue cell types are becoming increasingly important in advancing our understanding of cell biology and gene and protein expression. Prospective isolation of specific cell types is a powerful technique as it facilitates such investigations, allowing for analysis and characterization of individual cell populations. Such an approach to studying inner ear tissues presents a unique challenge because of the paucity of cells of interest and limited cell markers. In this chapter, we describe methods for selectively labeling and isolating different inner ear cell types from the neonatal mouse cochlea using fluorescence-activated cell sorting.
Department of Otolaryngology, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, MA, 02114, USA.
Taha A
TA
Jan
Department of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, 94305, USA.
Lina
L
Jansson
Department of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, 94305, USA.
Patrick J
PJ
Atkinson
Department of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, 94305, USA.
Tian
T
Wang
Department of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA, 94305, USA. aglcheng@stanford.edu.
Alan G
AG
Cheng
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002469:Cell Separation / Q000379:methods*; D003051:Cochlea / Q000166:cytology* / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods*; D051379:Mice; D008822:Mice, Transgenic; D059010:Single-Cell Analysis
D015415:Biomarkers
Fluorescence-activated cell sorting; Inner ear; Profiling; Single cell analysis