Pyrodictium delaneyi sp. nov., a hyperthermophilic autotrophic archaeon that reduces Fe(III) oxide and nitrate.
A hyperthermophilic, autotrophic iron and nitrate reducer, strain Su06T, was isolated from an active deep-sea hydrothermal vent chimney on the Endeavour Segment in the north-eastern Pacific Ocean. It was obligately anaerobic, hydrogenotrophic and reduced Fe(III) oxide to magnetite and NO3- to N2. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain was more than 97 % similar to other species of the genera Pyrodictium and Hyperthermus. Therefore, overall genome relatedness index analyses were performed to establish whether strain Su06T represents a novel species. For each analysis, strain Su06T was most similar to Pyrodictium occultum PL-19T. Relative to this strain, the average nucleotide identity score for strain Su06T was 72 %, the genome-to-genome direct comparison score was 13-19 % and the species identification score at the protein level was 89 %. For each analysis, strain Su06T was below the species delineation cutoff. Based on its whole genome sequence and its unique phenotypic characteristics, strain Su06T is suggested to represent a novel species of the genus Pyrodictium, for which the name Pyrodictium delaneyi is proposed. The type strain is Su06T (=DSM 28599T=ATCC BAA-2559T).
Department of Microbiology, University of Massachusetts, Amherst, MA 01003, USA.
T Jennifer
TJ
Lin
Department of Microbiology, University of Massachusetts, Amherst, MA 01003, USA.
Gabriel
G
El Sebae
Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup 580-185, Republic of Korea.
Jong-Hyun
JH
Jung
Graduate School of Biotechnology, Institute of Life Science and Resources, Kyung Hee University, Yongin 446-701, Republic of Korea.
Dong-Hyun
DH
Jung
Graduate School of Biotechnology, Institute of Life Science and Resources, Kyung Hee University, Yongin 446-701, Republic of Korea.
Cheon-Seok
CS
Park
Department of Microbiology, University of Massachusetts, Amherst, MA 01003, USA.
James F
JF
Holden
D001482:Base Composition; D019641:DNA, Archaeal / Q000235:genetics; D005290:Ferric Compounds / Q000378:metabolism*; D052203:Ferrosoferric Oxide / Q000378:metabolism; D060086:Hydrothermal Vents / Q000382:microbiology*; D007501:Iron / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism; D010140:Pacific Ocean; D010802:Phylogeny; D041523:Pyrodictiaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology; D017422:Sequence Analysis, DNA
D019641:DNA, Archaeal; D005290:Ferric Compounds; D009566:Nitrates; D012336:RNA, Ribosomal, 16S; C000499:ferric oxide; D007501:Iron; D052203:Ferrosoferric Oxide