Ligninolytic fungus Polyporus sp. S133 mediated metabolic degradation of fluorene.
This study aimed to investigate the impact of nonionic surfactants on the efficacy of fluorine degradation by Polyporus sp. S133 in a liquid culture. Fluorene was observed to be degraded in its entirety by Polyporus sp. S133 subsequent to a 23-day incubation period. The fastest cell growth rate was observed in the initial 7 days in the culture that was supplemented with Tween 80. The degradation process was primarily modulated by the activity of two ligninolytic enzymes, laccase and MnP. The highest laccase activity was stimulated by the addition of Tween 80 (2443U/L) followed by mixed surfactant (1766U/L) and Brij 35 (1655U/L). UV-vis spectroscopy, TLC analysis and mass spectrum analysis of samples subsequent to the degradation process in the culture medium confirmed the biotransformation of fluorene. Two metabolites, 9-fluorenol (λmax 270, tR 8.0min and m/z 254) and protocatechuic acid (λmax 260, tR 11.3min and m/z 370), were identified in the treated medium.
Centre for Environmental Sustainability and Water Security (IPASA), Research Institute for Sustainable Environment (RISE), Universiti Teknologi Malaysia, Skudai, Johor, Malaysia; Department of Environmental Engineering, Faculty of Civil Engineering, Universiti Teknologi Malaysia, Johor, Malaysia.
Zainab Mat
ZM
Lazim
Centre for Environmental Sustainability and Water Security (IPASA), Research Institute for Sustainable Environment (RISE), Universiti Teknologi Malaysia, Skudai, Johor, Malaysia; Department of Environmental Engineering, Faculty of Civil Engineering, Universiti Teknologi Malaysia, Johor, Malaysia. Electronic address: hadibarata@utm.my.
Tony
T
Hadibarata
D001673:Biodegradation, Environmental; D018533:Biomass; D001711:Biotransformation; D004785:Environmental Pollutants / Q000378:metabolism; D005449:Fluorenes / Q000737:chemistry / Q000378:metabolism*; D053858:Metabolic Networks and Pathways; D055442:Metabolome; D055432:Metabolomics / Q000379:methods; D055492:Polyporus / Q000201:enzymology / Q000378:metabolism*; D012995:Solubility
D004785:Environmental Pollutants; D005449:Fluorenes; C041509:fluorene
Ligninolytic enzymes; Metabolites; Non-ionic surfactants; Polyporus sp. S133; Solubilization