Proteomic analysis of stress-related proteins and metabolic pathways in Picea asperata somatic embryos during partial desiccation.
Partial desiccation treatment (PDT) stimulates germination and enhances the conversion of conifer somatic embryos. To better understand the mechanisms underlying the responses of somatic embryos to PDT, we used proteomic and physiological analyses to investigate these responses during PDT in Picea asperata. Comparative proteomic analysis revealed that, during PDT, stress-related proteins were mainly involved in osmosis, endogenous hormones, antioxidative proteins, molecular chaperones and defence-related proteins. Compared with those in cotyledonary embryos before PDT, these stress-related proteins remained at high levels on days 7 (D7) and 14 (D14) of PDT. The proteins that differentially accumulated in the somatic embryos on D7 were mapped to stress and/or stimuli. They may also be involved in the glyoxylate cycle and the chitin metabolic process. The most significant difference in the differentially accumulated proteins occurred in the metabolic pathways of photosynthesis on D14. Furthermore, in accordance with the changes in stress-related proteins, analyses of changes in water content, abscisic acid, indoleacetic acid and H2O2levels in the embryos indicated that PDT is involved in water-deficit tolerance and affects endogenous hormones. Our results provide insight into the mechanisms responsible for the transition from morphologically mature to physiologically mature somatic embryos during the PDT process in P. asperata.
State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.
Danlong
D
Jing
State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.
Jianwei
J
Zhang
State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.
Yan
Y
Xia
Department of Biology, Centre for Forest Biology, University of Victoria, Victoria, BC, Canada.
Lisheng
L
Kong
State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.
Fangqun
F
OuYang
State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.
Shougong
S
Zhang
State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.
Hanguo
H
Zhang
State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China.
Junhui
J
Wang
D000040:Abscisic Acid / Q000378:metabolism; D002686:Chitin / Q000378:metabolism; D018548:Cotyledon; D003890:Desiccation; D063990:Gene Ontology; D018525:Germination / Q000187:drug effects; D006360:Heat-Shock Proteins / Q000494:pharmacology* / Q000502:physiology; D006861:Hydrogen Peroxide / Q000378:metabolism; D007210:Indoleacetic Acids / Q000378:metabolism; D053858:Metabolic Networks and Pathways / Q000187:drug effects*; D009995:Osmosis; D010788:Photosynthesis / Q000187:drug effects; D028222:Picea / Q000033:anatomy & histology / Q000196:embryology* / Q000235:genetics / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D058439:Plant Somatic Embryogenesis Techniques / Q000379:methods; D040901:Proteomics; D012639:Seeds / Q000033:anatomy & histology / Q000187:drug effects* / Q000235:genetics / Q000502:physiology*; D014867:Water / Q000737:chemistry
D006360:Heat-Shock Proteins; D007210:Indoleacetic Acids; D010940:Plant Proteins; D014867:Water; D002686:Chitin; C030737:indoleacetic acid; D000040:Abscisic Acid; D006861:Hydrogen Peroxide

Picea asperata
; partial desiccation treatment; proteomics; somatic embryo; stress-related protein