Reassortment compatibility between PB1, PB2, and HA genes of the two influenza B virus lineages in mammalian cells.
In addition to influenza A subtypes, two distinct lineages of influenza B virus also cause seasonal epidemics to humans. Recently, Dudas et al. have done evolutionary analyses of reassortment patterns of the virus and suggested genetic lineage relationship between PB1, PB2, and HA genes. Using genetic plasmids and reassortant viruses, we here demonstrate that a homologous lineage PB1-PB2 pair exhibits better compatibility than a heterologous one and that the lineage relationship between PB1 and HA is more important for viral replication than that between PB2 and HA. However, co-adaptation of PB1-PB2-HA genes appears to be affected by complete gene constellation.
Department of Microbiology, the Institute of Viral Diseases, College of Medicine, Korea University, Seoul 02841, Republic of Korea.
Jin Il
JI
Kim
Department of Microbiology, the Institute of Viral Diseases, College of Medicine, Korea University, Seoul 02841, Republic of Korea.
Ilseob
I
Lee
Department of Microbiology, the Institute of Viral Diseases, College of Medicine, Korea University, Seoul 02841, Republic of Korea.
Sehee
S
Park
Department of Microbiology, the Institute of Viral Diseases, College of Medicine, Korea University, Seoul 02841, Republic of Korea.
Joon-Yong
JY
Bae
Department of Microbiology, the Institute of Viral Diseases, College of Medicine, Korea University, Seoul 02841, Republic of Korea.
Kirim
K
Yoo
Department of Microbiology and Immunology, Rega Institute, KU Leuven - University of Leuven, Leuven 3000, Belgium.
Philippe
P
Lemey
Department of Microbiology, the Institute of Viral Diseases, College of Medicine, Korea University, Seoul 02841, Republic of Korea.
Mee Sook
MS
Park
Department of Microbiology, the Institute of Viral Diseases, College of Medicine, Korea University, Seoul 02841, Republic of Korea.
Jin-Won
JW
Song
Department of Microbiology, the Institute of Viral Diseases, College of Medicine, Korea University, Seoul 02841, Republic of Korea.
Sun-Ho
SH
Kee
Department of Microbiology, the Institute of Viral Diseases, College of Medicine, Korea University, Seoul 02841, Republic of Korea.
Ki-Joon
KJ
Song
Department of Microbiology, the Institute of Viral Diseases, College of Medicine, Korea University, Seoul 02841, Republic of Korea.
Man-Seong
MS
Park
D000818:Animals; D005814:Genes, Viral; D009981:Influenza B virus / Q000235:genetics* / Q000502:physiology; D010802:Phylogeny; D016865:Reassortant Viruses / Q000235:genetics* / Q000502:physiology; D014779:Virus Replication