Prevalence of sorbitol non-fermenting Shiga toxin-producing Escherichia coli in Black Bengal goats on smallholdings.
A cross-sectional survey was carried out in Bangladesh with the sampling of 514 Black Bengal goats on smallholdings to determine the presence of sorbitol non-fermenting (SNF) Shiga toxin-producing E. coli (STEC). Swab samples collected from the recto-anal junction were plated onto cefixime and potassium tellurite added sorbitol MacConkey (CT-SMAC) agar, a selective medium for STEC O157 serogroup, where this serogroup and other SNF STEC produce colourless colonies. The SNF E. coli (SNF EC) isolates obtained from the survey were investigated by PCR for the presence of Shiga toxin-producing genes, stx1 and stx2, and two other virulence genes, eae and hlyA that code for adherence factor (intimin protein) and pore-forming cytolysin, respectively. The SNF EC isolates were also assessed for the presence of the rfbO157 gene to verify their identity to O157 serogroup. The results revealed that the proportions of goats carrying SNF EC isolates and stx1 and stx2 genes were 6·2% (32/514) [95% confidence interval (CI) 4·4-8·7)], 1·2% (95% CI 0·5-2·6) and 1·2% (95% CI 0·5-2·6), respectively. All the SNF STEC tested negative for rfbO157, hlyA and eae genes. The risk for transmission of STEC from Black Bengal goats to humans is low.
Department of Microbiology and Veterinary Public Health,Chittagong Veterinary and Animal Sciences University,Bangladesh.
M
M
Das Gupta
Department of Genetics and Animal Breeding,Chittagong Veterinary and Animal Sciences University,Bangladesh.
A
A
Das
Department of Microbiology and Veterinary Public Health,Chittagong Veterinary and Animal Sciences University,Bangladesh.
M Z
MZ
Islam
Department of Microbiology and Veterinary Public Health,Chittagong Veterinary and Animal Sciences University,Bangladesh.
P K
PK
Biswas
D000818:Animals; D001459:Bangladesh / Q000453:epidemiology; D003430:Cross-Sectional Studies; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D029968:Escherichia coli Proteins / Q000235:genetics; D005260:Female; D015511:Goat Diseases / Q000453:epidemiology* / Q000382:microbiology*; D006041:Goats; D008297:Male; D015995:Prevalence; D054323:Shiga-Toxigenic Escherichia coli / Q000145:classification / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D013012:Sorbitol / Q000378:metabolism; D014774:Virulence / Q000235:genetics
D029968:Escherichia coli Proteins; D013012:Sorbitol
Black Bengal goats; E coli; shiga toxin