Preparation of Prunella vulgaris polysaccharide-zinc complex and its antiproliferative activity in HepG2 cells.
Prunella vulgaris polysaccharides have been reported to have antioxidant, antitumor and immunomodulatory activities. In this study, P. vulgaris polysaccharide (P1)-zinc complex (P1-Zn) was first prepared by a facile method and its antiproliferative effect on HepG2 human hepatocellular carcinoma cells was also investigated. Results showed that P1-Zn could effectively inhibit the proliferation (98.4% inhibition rate at 500μg/mL) of HepG2 cells through induction of apoptosis, evidenced by morphological changes, chromatin condensation and G0/G1 phase cell cycle arrest. The intracellular mechanism of P1-Zn induced apoptosis was found to be the involvement of the activation of caspase-3 and -9, reactive oxygen species (ROS) overproduction and mitochondrial dysfunction. Our findings suggest that P1-Zn may be a potent candidate for human hepatocellular carcinoma treatment and prevention in functional foods and pharmacological fields.
School of Food Science and Technology, South China University of Technology, 381 Wushan Road, Guangzhou 510640, China.
Chao
C
Li
School of Food Science and Technology, South China University of Technology, 381 Wushan Road, Guangzhou 510640, China.
Qiang
Q
Huang
Department of Food Science, Rutgers University, 65 Dudley Road, New Brunswick, NJ 08901, USA.
Jie
J
Xiao
School of Food Science and Technology, South China University of Technology, 381 Wushan Road, Guangzhou 510640, China. Electronic address: lfxfu@scut.edu.cn.
Xiong
X
Fu
School of Food Science and Technology, South China University of Technology, 381 Wushan Road, Guangzhou 510640, China. Electronic address: feyoulijun@scut.edu.cn.
Lijun
L
You
School of Food Science and Technology, South China University of Technology, 381 Wushan Road, Guangzhou 510640, China; Department of Food Science, Stocking Hall, Cornell University, Ithaca, NY 14853, USA.
Rui Hai
RH
Liu
D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D004553:Electric Conductivity; D056945:Hep G2 Cells; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D011134:Polysaccharides / Q000494:pharmacology*; D031347:Prunella / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D015032:Zinc / Q000494:pharmacology*
D011134:Polysaccharides; D017382:Reactive Oxygen Species; D053148:Caspase 3; D053453:Caspase 9; D015032:Zinc
Apoptosis; Prunella vulgaris polysaccharide; Zinc acetate