Apple phenolics as inhibitors of the carbonylation pathway during in vitro metal-catalyzed oxidation of myofibrillar proteins.
The effect of apple phenolics on the oxidative damage caused to myofibrillar proteins by an in vitro metal-catalyzed oxidation system was investigated. Three pure phenolic compounds (chlorogenic acid, (-)-epicatechin and phloridzin) and an apple peel extract were added to myofibrillar proteins in three concentrations (50, 100 and 200μM), and a blank treatment was included as a control. All suspensions were subjected to Fe(3+)/H2O2 oxidation at 37°C during 10days, and protein oxidation was evaluated as carbonylation (α-amino adipic and γ-glutamic semialdehydes) and Schiff base cross-links. Significant inhibition by apple phenolics was found as compared to the control treatment, with (-)-epicatechin being the most efficient antioxidant and phloridzin showing the weakest antioxidant effect. The higher concentrations of apple extract showed effective antioxidant activity against protein oxidation in myofibrillar proteins, emphasizing the potential of apple by-products as natural inhibitors of protein oxidation in meat products.
Technology and Food Science Unit, Institute for Agricultural and Fisheries Research, Brusselsesteenweg 370, 9090 Melle, Belgium. Electronic address: tine.rysman@ilvo.vlaanderen.be.
Tine
T
Rysman
IPROCAR Research Institute, University of Extremadura, Avda. Universidad, s/n, 10003 Cáceres, Spain. Electronic address: mutrera@unex.es.
Mariana
M
Utrera
IPROCAR Research Institute, University of Extremadura, Avda. Universidad, s/n, 10003 Cáceres, Spain. Electronic address: demorcuen@unex.es.
David
D
Morcuende
Technology and Food Science Unit, Institute for Agricultural and Fisheries Research, Brusselsesteenweg 370, 9090 Melle, Belgium. Electronic address: geert.vanroyen@ilvo.vlaanderen.be.
Geert
G
Van Royen
Technology and Food Science Unit, Institute for Agricultural and Fisheries Research, Brusselsesteenweg 370, 9090 Melle, Belgium. Electronic address: stephanie.vanweyenberg@ilvo.vlaanderen.be.
Stephanie
S
Van Weyenberg
Laboratory for Animal Nutrition and Animal Product Quality, Faculty of Bioscience Engineering, Ghent University, Proefhoevestraat 10, 9090 Melle, Belgium. Electronic address: stefaan.desmet@ugent.be.
Stefaan
S
De Smet
IPROCAR Research Institute, University of Extremadura, Avda. Universidad, s/n, 10003 Cáceres, Spain. Electronic address: mariovet@unex.es.
Mario
M
Estévez
D000975:Antioxidants / Q000494:pharmacology; D002384:Catalysis; D027845:Malus / Q000737:chemistry*; D008670:Metals / Q000494:pharmacology; D009210:Myofibrils / Q000737:chemistry*; D010084:Oxidation-Reduction; D010636:Phenols / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology; D050050:Protein Carbonylation / Q000187:drug effects*
D000975:Antioxidants; D008670:Metals; D010636:Phenols; D010936:Plant Extracts
(−)-Epicatechin; (−)-Epicatechin (PubChem CID: 72276); Chlorogenic acid; Chlorogenic acid (PubChem CID: 1794427); Phloridzin; Phloridzin (PubChem CID: 6072); Schiff base structures; α-Aminoadipic semialdehyde; γ-Glutamic semialdehyde; γ-Glutamic semialdehyde (PubChem CID: 193305)