Blocking of TRPV-1 in the parodontium relieves orthodontic pain by inhibiting the expression of TRPV-1 in the trigeminal ganglion during experimental tooth movement in rats.
Orthodontic pain has confused the orthodontics for a long time, and recent research demonstrated that transient receptor potential vanilloid type 1 (TRPV1) had crucial functions in transduction of painful stimuli. The present research investigated the analgesia effects of the blocking TRPV1 on orthodontic pain during experimental tooth movement. Under challenge with experimental tooth movement, the expression of TRPV1 in the parodontium was increased in a time-dependent and force-dependent manner. And treatment with selective TRPV1 antagonist AMG-9810 in the parodontium reduced the expression of TRPV1 in the trigeminal ganglion (TG) and decreased the secretion of IL-1β in the gingival crevicular fluid. Furthermore, AMG-9810 could relieve orthodontic pain arising from experimental tooth movement in rats. We suggest that TRPV1 both in the parodontium and trigeminal ganglion are involved in orthodontic pain, and TRPV1 in the parodontium influence on orthodontic pain through reducing the expression of TRPV1 in trigeminal ganglion. Our finding may help to develop strategies for relieving orthodontic pain after orthodontics.
Department of Orthodontics, The Affiliated Stomatological Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, China; Institute of Neurobiology, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, China.
Yunan
Y
Gao
Institute of Neurobiology, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, China.
Yingfei
Y
Liu
Institute of Neurobiology, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, China.
Kun
K
Zhu
Institute of Neurobiology, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, China.
Zhichao
Z
Zhang
Department of Orthodontics, The Affiliated Stomatological Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, China.
Hu
H
Qiao
Department of Orthodontics, The Affiliated Stomatological Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, China.
Zhen
Z
Lu
Department of Orthodontics, The Affiliated Stomatological Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, China; Institute of Neurobiology, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, China.
Tianyu
T
Zhong
Institute of Neurobiology, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi 710061, China. Electronic address: liuy5599@mail.xjtu.edu.cn.
Yong
Y
Liu
Department of Orthodontics, The Affiliated Stomatological Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, China. Electronic address: zhouhong@mail.xjtu.edu.cn.
Hong
H
Zhou
D000178:Acrylamides / Q000008:administration & dosage; D000818:Animals; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000008:administration & dosage; D005883:Gingival Crevicular Fluid / Q000378:metabolism; D006120:Grooming / Q000187:drug effects; D053583:Interleukin-1beta / Q000378:metabolism; D008297:Male; D010146:Pain / Q000378:metabolism*; D010147:Pain Measurement; D010519:Periodontium / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D050916:TRPV Cation Channels / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D014070:Tooth / Q000503:physiopathology*; D012668:Trigeminal Ganglion / Q000378:metabolism*
C500530:3-(4-t-butylphenyl)-N-(2,3-dihydrobenzo(b)(1,4)dioxin-6-yl)acrylamide; D000178:Acrylamides; D019086:Bridged Bicyclo Compounds, Heterocyclic; D053583:Interleukin-1beta; D050916:TRPV Cation Channels; C493922:Trpv1 protein, rat
Experimental tooth movement; Orthodontic pain; Parodontium; Transient receptor potential vanilloid type 1