Scanning electron microscopy combined with image processing technique: Analysis of microstructure, texture and tenderness in Semitendinous and Gluteus Medius bovine muscles.
In this study the effect of freeze drying on the microstructure, texture, and tenderness of Semitendinous and Gluteus Medius bovine muscles were analyzed applying Scanning Electron Microscopy combined with image analysis. Samples were analyzed by Scanning Electron Microscopy at different magnifications (250, 500, and 1,000×). Texture parameters were analyzed by Texture analyzer and by image analysis. Tenderness by Warner-Bratzler shear force. Significant differences (p < 0.05) were obtained for image and instrumental texture features. A linear trend with a linear correlation was applied for instrumental and image features. Image texture features calculated from Gray Level Co-occurrence Matrix (homogeneity, contrast, entropy, correlation and energy) at 1,000× in both muscles had high correlations with instrumental features (chewiness, hardness, cohesiveness, and springiness). Tenderness showed a positive correlation in both muscles with image features (energy and homogeneity). Combing Scanning Electron Microscopy with image analysis can be a useful tool to analyze quality parameters in meat.Summary SCANNING 38:727-734, 2016. © 2016 Wiley Periodicals, Inc.
CINSO-UNIDEF (Strategic I & D for Defense)-MINDEF-CITEDEF-CONICET, Villa Martelli, Buenos Aires, Argentina.
Facundo
F
Pieniazek
CINSO-UNIDEF (Strategic I & D for Defense)-MINDEF-CITEDEF-CONICET, Villa Martelli, Buenos Aires, Argentina.|The National Council for Scientific and Technical Research (CONICET), Rivadavia, Buenos Aires, Argentina.
Valeria
V
Messina
D000818:Animals; D002417:Cattle; D005612:Freeze Drying; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008460:Meat; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D018482:Muscle, Skeletal / Q000648:ultrastructure*
image analysis; muscle; scanning electron microscopy; surface analysis