Simultaneous extraction and determination of albendazole and triclabendazole by a novel syringe to syringe dispersive liquid phase microextraction-solidified floating organic drop combined with high performance liquid chromatography.
A syringe to syringe dispersive liquid phase microextraction-solidified floating organic drop was introduced and used for the simultaneous extraction of trace amounts of albendazole and triclabendazole from different matrices. The extracted analytes were determined by high performance liquid chromatography along with fluorescence detection. The analytical parameters affecting the microextraction efficiency including the nature and volume of the extraction solvent, sample volume, sample pH, ionic strength and the cycles of extraction were optimized. The calibration curves were linear in the range of 0.1-30.0 μg L(-1) and 0.2-30.0 μg L(-1) with determination coefficients of 0.9999 and 0.9998 for albendazole and triclabendazole respectively. The detection limits defined as three folds of the signal to noise ratio were found to be 0.02 μg L(-1) for albendazole and 0.06 μg L(-1) for triclabendazole. The inter-day and intra-day precision (RSD%) for both analytes at three concentration levels (0.5, 2.0 and 10.0 μg L(-1)) were in the range of 6.3-10.1% and 5.0-7.5% respectively. The developed method was successfully applied to determine albendazole and triclabendazole in water, cow milk, honey, and urine samples.
Department of Chemistry, Faculty of Science, Yazd University, Yazd 89195-741, Iran.
Mohammad
M
Asadi
Department of Chemistry, Faculty of Science, Yazd University, Yazd 89195-741, Iran. Electronic address: sdadfarnia@yazd.ac.ir.
Shayessteh
S
Dadfarnia
Department of Chemistry, Faculty of Science, Yazd University, Yazd 89195-741, Iran.
Ali Mohammad
AM
Haji Shabani
D015766:Albendazole / Q000032:analysis* / Q000302:isolation & purification*; D001562:Benzimidazoles / Q000032:analysis* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D059627:Liquid Phase Microextraction / Q000295:instrumentation*; D009994:Osmolar Concentration; D012997:Solvents / Q000737:chemistry; D013594:Syringes; D013997:Time Factors; D000077682:Triclabendazole
D001562:Benzimidazoles; D012997:Solvents; D000077682:Triclabendazole; D015766:Albendazole
Albendazole; High performance liquid chromatography; Syringe to syringe dispersive liquid phase microextraction-solidified floating organic drop; Triclabendazole