A protein-repellent and antibacterial nanocomposite for Class-V restorations to inhibit periodontitis-related pathogens.
The objectives of this study were to develop a bioactive dental composite and investigate the effects of 2-methacryloyloxyethyl phosphorylcholine (MPC) and dimethylaminohexadecyl methacrylate (DMAHDM) in Class V composite on mechanical properties, water sorption, protein adsorption, and inhibition of four species of periodontitis-related biofilms for the first time. The resin consisted of ethoxylated bisphenol A dimethacrylate (EBPADMA) and pyromellitic glycerol dimethacrylate (PMGDM). DMAHDM, MPC and nanoparticles of amorphous calcium phosphate (NACP) were incorporated into the resin. Four species (Porphyromonas gingivalis, Prevotella intermedia, Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum) were tested for biofilm colony-forming units (CFU), live/dead, metabolic activity, and polysaccharide production. The results showed that adding DMAHDM and MPC to the composite did not compromise the mechanical properties (p>0.1), with acceptable water sorption values. Composite with 3% MPC reduced protein adsorption to 1/9 that of a commercial composite (p<0.05). For all four species, the composite with 3% DMAHDM+3% MPC had much greater reduction in biofilms than using DMAHDM or MPC alone (p<0.05). Biofilm CFU was reduced by about 4 orders of magnitude via 3% DMAHDM+3% MPC, compared to control. The inhibition efficacy for the four species was: P. gingivalis>P intermedia=A. actinomycetemcomitans>F. nucleatum. In conclusion, a novel bioactive composite with 3% DMAHDM and 3% MPC achieved the greatest reduction in biofilm growth, metabolic activity and polysaccharide of four periodontal pathogens. The new composite is promising for Class V restorations especially with subgingival margins to inhibit periodontal pathogens, combat periodontitis and protect the periodontium.
VIP Integrated Department, School and Hospital of Stomatology, Jilin University, Changchun 130011, China; Department of Endodontics, Periodontics and Prosthodontics, University of Maryland School of Dentistry, Baltimore, MD 21201, USA. Electronic address: wanglin1982@jlu.edu.cn.
Lin
L
Wang
Department of Endodontics, Periodontics and Prosthodontics, University of Maryland School of Dentistry, Baltimore, MD 21201, USA; Department of Orthodontics, School of Stomatology, Capital Medical University, Beijing, China.
Xianju
X
Xie
Department of Biomaterials Science, Osaka University Graduate School of Dentistry, Osaka, Japan.
Satoshi
S
Imazato
Department of Endodontics, Periodontics and Prosthodontics, University of Maryland School of Dentistry, Baltimore, MD 21201, USA.
Michael D
MD
Weir
Department of Endodontics, Periodontics and Prosthodontics, University of Maryland School of Dentistry, Baltimore, MD 21201, USA.
Mark A
MA
Reynolds
Department of Endodontics, Periodontics and Prosthodontics, University of Maryland School of Dentistry, Baltimore, MD 21201, USA; Center for Stem Cell Biology & Regenerative Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA; Department of Mechanical Engineering, University of Maryland Baltimore County, Baltimore County, MD 21250, USA. Electronic address: hxu@umaryland.edu.
Hockin H K
HHK
Xu
D000327:Adsorption; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000493:pharmacokinetics; D001419:Bacteria; D018407:Bacterial Physiological Phenomena; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D002417:Cattle; D053761:Nanocomposites / Q000737:chemistry*; D010518:Periodontitis / Q000188:drug therapy / Q000382:microbiology*; D012710:Serum Albumin, Bovine / Q000737:chemistry*
D000900:Anti-Bacterial Agents; D012710:Serum Albumin, Bovine
Antibacterial; Calcium phosphate nanoparticle; Class V restoration; Dental composite; Periodontal pathogens; Protein repellent