Characterization and preparation of oligomeric procyanidins from Litchi chinensis pericarp.
The main purpose of this study is to characterize and prepare A-type oligomeric procyanidins from litchi pericarp (Litchi chinensis Baila). The variety of oligomeric procyanidins was characterized by LC-ESI-MS analysis. There were (+)-catechin, (-)-epicatechin, twelve dimers and six trimers of procyanidins were found in litchi pericarp extracts, and A-type procyanidins were much more abundant than B-type procyanidins. The main flavan-3-ol monomer and oligomeric procyanidins in litchi pericarp were (-)-epicatechin, A-type dimers (A1 and A2) and trimer (epicatechin-(4β-8, 2β-O-7)-epicatechin- (4β-8)-epicatechin). Procyanidin A1 (epicatechin-(4β-8, 2β-O-7)-catechin) was identified by NMR in litchi pericarp for the first time. (-)-Epicatechin and oligomeric procyanidins were prepared by the combination of AB-8 column chromatography and Toyopearl HW-40S column chromatography. The results showed that each fraction predominantly owned a single compound and gave a high yield with (-)-epicatechin, A-type dimers (A1 and A2) and trimer, suggesting a useful method to obtain pure (-)-epicatechin and A-type oligomeric procyanidins.
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China; Key Laboratory of Environment Correlative Dietology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
Yong
Y
Sui
College of Food and Biology Science and Technology, Wuhan Institute of Design and Science, Wuhan 430205, People's Republic of China.
Yan
Y
Zheng
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
Xiaopeng
X
Li
College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, People's Republic of China.
Shuyi
S
Li
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
Bijun
B
Xie
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, People's Republic of China. Electronic address: sunzhida@sina.com.
Zhida
Z
Sun
D044946:Biflavonoids / Q000737:chemistry* / Q000302:isolation & purification; D002392:Catechin / Q000737:chemistry* / Q000302:isolation & purification; D005638:Fruit / Q000737:chemistry*; D032125:Litchi / Q000737:chemistry*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry; D044945:Proanthocyanidins / Q000737:chemistry* / Q000302:isolation & purification
D044946:Biflavonoids; D010936:Plant Extracts; D044945:Proanthocyanidins; C520684:procyanidin A1; C479578:procyanidin B; D002392:Catechin
Characterization; Litchi pericarp; Oligomeric procyanidins; Preparation