Optimization of nonribosomal peptides production by a psychrotrophic fungus: Trichoderma velutinum ACR-P1.
Trichoderma is an anamorphic filamentous fungal genus with immense potential for production of small valuable secondary metabolites with indispensable biological activities. Microbial dynamics of a psychrotrophic strain Trichoderma velutinum ACR-P1, isolated from unexplored niches of the Shiwalik region, bestowed with rich biodiversity of microflora, was investigated for production of nonribosomal peptides (NRPs) by metabolite profiling by intact-cell mass spectrometry (ICMS) employing matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometer. Being the first report on NRPs production by T. velutinum, studies on optimization of growth conditions by Response Surface Methodology (RSM) for production of NRPs by ACR-P1 was carried out strategically. Multifold enhancement in the yield of NRPs belonging to subfamily SF4 with medium chain of amino acid residues having m/z 1437.9, 1453.9, and 1452.0 at pH 5.9 at 20 °C and of subfamily SF1 with long-chain amino acid residues having m/z 1770.2, 1784.2, 1800.1, 1802.1, and 1815.1 was achieved at pH 7.0 at 25 °C. Complexities of natural mixtures were thus considerably reduced under respective optimized culture conditions accelerating the production of novel microbial natural products by saving time and resources.
Fermentation Technology Division, CSIR-Indian Institute of Integrative Medicine, Jammu, 180001, India.|Academy of Scientific and Innovative Research, New Delhi, 110001, India.
Richa
R
Sharma
Medicinal Chemistry Division, CSIR-Indian Institute of Integrative Medicine, Jammu, 180001, India.|Faculty of Sciences, Department of Biotechnology, Shri Mata Vaishno Devi University, Katra, 182320, India.
Varun P
VP
Singh
Medicinal Chemistry Division, CSIR-Indian Institute of Integrative Medicine, Jammu, 180001, India. dsingh@iiim.ac.in.|Academy of Scientific and Innovative Research, New Delhi, 110001, India. dsingh@iiim.ac.in.
Deepika
D
Singh
Fermentation Technology Division, CSIR-Indian Institute of Integrative Medicine, Jammu, 180001, India.
Farnaz
F
Yusuf
Faculty of Sciences, Department of Biotechnology, Shri Mata Vaishno Devi University, Katra, 182320, India.
Anil
A
Kumar
Medicinal Chemistry Division, CSIR-Indian Institute of Integrative Medicine, Jammu, 180001, India.|Academy of Scientific and Innovative Research, New Delhi, 110001, India.
Ram A
RA
Vishwakarma
Fermentation Technology Division, CSIR-Indian Institute of Integrative Medicine, Jammu, 180001, India. achaubey@iiim.ac.in.|Academy of Scientific and Innovative Research, New Delhi, 110001, India. achaubey@iiim.ac.in.
Asha
A
Chaubey
D001688:Biological Products / Q000378:metabolism*; D004783:Environmental Microbiology; D006863:Hydrogen-Ion Concentration; D046908:Peptide Biosynthesis, Nucleic Acid-Independent; D010455:Peptides / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013696:Temperature; D014242:Trichoderma / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
D001688:Biological Products; D010455:Peptides
ICMS; MALDI-TOF MS; Nonribosomal peptides (NRPs); Optimization; RSM; Shiwalik region; Trichoderma velutinum