Comparison of multilocus sequence typing and multilocus typing microarray of Chlamydia trachomatis strains from Argentina and Chile.
This study compared conventional ompA genotyping of Chlamydia trachomatis with multilocus sequence typing (MLST) and multilocus typing (MLT) DNA microarray.

DNA extracts of 104 C. trachomatis positive specimens were analyzed by ompA sequencing and MLST and of these 76 by MLT array. Obtained MLST sequence types (STs) were compared to sequences in the database http://mlstdb.uu.se. The resolution obtained for MLST (35 STs) was 2.1 higher than for ompA sequencing (17 variants) and 1.3 higher than MLT array (27 MLT groups). Among the 104 samples the predominant genotype E could be divided into 5 ompA variants and 23 STs of which 16 had not been reported in previous studies. The most common STs, ST3 and ST56, were identified as founders and are common in several countries on a global scale. The MLST and the MLT array provided similar strain discrimination capacity and showed considerably higher resolution than conventional ompA sequencing.
Section of Clinical Bacteriology, Department of Medical Sciences, Uppsala University, S-751 85 Uppsala, Sweden. Electronic address: jenny.isaksson@medsci.uu.se.
Jenny
J
Isaksson
Universidad de Buenos Aires, Cátedra de Microbiología Clínica, Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina, Junin 956, CP1113 Ciudad Autónoma de Buenos Aires, Argentina. Electronic address: luligv@hotmail.com.
Lucía
L
Gallo Vaulet
Section of Clinical Bacteriology, Department of Medical Sciences, Uppsala University, S-751 85 Uppsala, Sweden. Electronic address: justus007@hotmail.com.
Linus
L
Christerson
Institute of Molecular Pathogenesis, Friedrich-Loeffler-Institut (Federal Research Institute for Animal Health), D-07743 Jena, Germany. Electronic address: anke.ruettger@fli.bund.de.
Anke
A
Ruettger
Institute of Molecular Pathogenesis, Friedrich-Loeffler-Institut (Federal Research Institute for Animal Health), D-07743 Jena, Germany. Electronic address: konrad.sachse@gmx.net.
Konrad
K
Sachse
Universidad de Buenos Aires, Cátedra de Microbiología Clínica, Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina, Junin 956, CP1113 Ciudad Autónoma de Buenos Aires, Argentina. Electronic address: carolinaentrocassi@yahoo.com.
Carolina
C
Entrocassi
Facultad de Medicina, Universidad de San Sebastián, Chile, Lientur 1457, Concepción, Chile. Electronic address: ercastroh@gmail.com.
Érica
É
Castro
Universidad de Buenos Aires, Cátedra de Microbiología Clínica, Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina, Junin 956, CP1113 Ciudad Autónoma de Buenos Aires, Argentina. Electronic address: mrfchlam@ffyb.uba.ar.
Marcelo
M
Rodríguez Fermepin
Section of Clinical Bacteriology, Department of Medical Sciences, Uppsala University, S-751 85 Uppsala, Sweden. Electronic address: bjorn.herrmann@medsci.uu.se.
Björn
B
Herrmann
D001118:Argentina; D002677:Chile; D002692:Chlamydia trachomatis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005838:Genotype; D058889:Molecular Typing / Q000379:methods*
Chlamydia trachomatis; DNA array; Genotyping; MLST; ompA