Isolation and genetic characterization of swinepox virus from pigs in India.
Swinepox virus (SWPV), a member of the genus Suipoxvirus causes generalized pock-like lesions on the body of domestic and wild pigs. Although outbreak has been reported in India since 1987, virus isolation and genetic characterization remained elusive. In September 2013, an outbreak of acute skin infection occurred in piglets in a commercial piggery unit at Rohtak district in Haryana, India. The presence of SWPV in scab samples collected from piglets succumbed to infection was confirmed by virus isolation, PCR amplification of SWPV-specific gene segments and nucleotide sequencing. Phylogenetic analysis of host-range genes of the SWPV revealed that the Indian isolate is genetically closely related to reference isolate SWPV/pig/U.S.A/1999/Nebraska. To the best of our knowledge this is the first report on isolation and genetic characterization of SWPV from pigs in India.
ICAR-National Research Centre on Equines, National Centre for Veterinary Type Culture Collection, Hisar, Haryana-125001, India. Electronic address: sbarua06@gmail.com.
Thachamvally
T
Riyesh
ICAR-National Research Centre on Equines, National Centre for Veterinary Type Culture Collection, Hisar, Haryana-125001, India.
Sanjay
S
Barua
ICAR-National Research Centre on Equines, National Centre for Veterinary Type Culture Collection, Hisar, Haryana-125001, India.
Naveen
N
Kumar
Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana-125004, India.
Naresh
N
Jindal
ICAR-National Research Centre on Equines, National Centre for Veterinary Type Culture Collection, Hisar, Haryana-125001, India.
Bidhan Chandra
BC
Bera
Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana-125004, India.
Gulshan
G
Narang
Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana-125004, India.
Nand Kishore
NK
Mahajan
Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana-125004, India.
Devan
D
Arora
ICAR-National Research Centre on Equines, National Centre for Veterinary Type Culture Collection, Hisar, Haryana-125001, India.
Taruna
T
Anand
ICAR-National Research Centre on Equines, National Centre for Veterinary Type Culture Collection, Hisar, Haryana-125001, India.
Rajesh Kumar
RK
Vaid
ICAR-National Research Centre on Equines, National Centre for Veterinary Type Culture Collection, Hisar, Haryana-125001, India.
Mansi
M
Yadav
ICAR-National Research Centre on Equines, National Centre for Veterinary Type Culture Collection, Hisar, Haryana-125001, India.
Surender Singh
SS
Chandel
C.C.S. National Institute of Animal Health, Baghpat, Uttar Pradesh-250609, India.
Praveen
P
Malik
ICAR-National Research Centre on Equines, National Centre for Veterinary Type Culture Collection, Hisar, Haryana-125001, India.
Bhupendra Nath
BN
Tripathi
Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh-243122, India.
Raj Kumar
RK
Singh
D000818:Animals; D017089:Ankyrin Repeat / Q000235:genetics; D001483:Base Sequence; D004196:Disease Outbreaks; D058507:Host Specificity; D007194:India / Q000453:epidemiology; D010802:Phylogeny; D011213:Poxviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D018148:Suipoxvirus / Q000235:genetics* / Q000302:isolation & purification*; D013552:Swine / Q000821:virology; D013553:Swine Diseases / Q000821:virology*
Ankyrin- repeat protein; Extracellular enveloped protein; Host-range genes; Kelch-like protein; Swinepox virus