Estimation of Biocompatibility of Nano-Sized Ceramic Particles with Osteoblasts, Osteosarcomas and Hepatocytes by Static and Time-Lapse Observation.
We assessed the biocompatibility of nano-sized ceramic particles with several cells types. Though these particles have less than 100 nm in diameter, they act as submicron-sized particles in saline by aggregation that was estimated using laser diffraction particle size analysis (LDS). they act as submicro-sized particles in saline by aggregation based on laser diffraction particle size analysis (LDS). Several types of cells (osteoblasts, osteosarcoma and hepatocyte cells) were exposed to these particles and their cytocompatibility was estimated. Not only the cytotoxic assay but also their static and dynamic morphology under nanoparticles exposure were investigated. The intercellular uptake of particles was determined using a confocal fluorescence microscope. The particles used in this study did not inhibit cellular activity or growth even when their concentrations were high. Only copper oxide particles caused acute cytotoxicity depending on the particle size. The cytotoxicity assay, dynamic behavior of the nanoparticle-exposed cells and their examination under a confocal fluorescence microscope suggests that the irritative reaction was induced by contact between the cells and particles, whereas eluted copper ions are not dominant factor. These results indicate that nano-sized particles used in this study have excellent biocompatibility except copper oxide ones.
Shigeaki
S
Abe
Eri
E
Seitoku
Nobuki
N
Iwadera
Yusuke
Y
Hamba
Shuichi
S
Yamagata
Tsukasa
T
Akasaka
Teruo
T
Kusaka
Satoshi
S
Inoue
Yasutaka
Y
Yawaka
Junichiro
J
Iida
Hidehiko
H
Sano
Tetsu
T
Yonezawa
Yasuhiro
Y
Yoshida
D000818:Animals; D001672:Biocompatible Materials / Q000633:toxicity; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D002516:Ceramics / Q000633:toxicity*; D022781:Hepatocytes / Q000187:drug effects* / Q000473:pathology; D006801:Humans; D051379:Mice; D018715:Microscopy, Video / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry / Q000633:toxicity* / Q000648:ultrastructure; D010006:Osteoblasts / Q000187:drug effects* / Q000473:pathology; D012516:Osteosarcoma / Q000473:pathology*; D010316:Particle Size; D059008:Time-Lapse Imaging / Q000379:methods
D001672:Biocompatible Materials