Purification and characterisation of a novel angiotensin-I converting enzyme (ACE)-inhibitory peptide derived from the enzymatic hydrolysate of Enteromorpha clathrata protein.
Hydrolysates containing angiotensin-I converting enzyme (ACE)-inhibitory peptide were prepared from Enteromorpha clathrata protein using alcalase. The hydrolysates were fractionated into two molecular-weight ranges (below and above 10kDa) by ultrafiltration. The below-10kDa fraction showed higher ACE-inhibitory activity and was subsequently purified by Sephadex G-15 gel filtration chromatography. The structure of active peptide was identified as Pro-Ala-Phe-Gly by HPLC-Q-TOF-MS and its IC50 value was 35.9μM. The yield of this peptide from E. clathrata protein was 0.82%. Lineweaver-Burk plots demonstrated that the inhibitory kinetic mechanism of this peptide was non-competitive. Stability study revealed that the purified peptide showed resistance against gastrointestinal proteases. Thus, E. clathrata protein hydrolysate treated with alcalase is a beneficial ingredient of nutraceuticals and pharmaceuticals against hypertension and related diseases.
Jiangsu Marine Resources Development Research Institute, Lianyungang, Jiangsu 222005, China; School of Marine Science and Technology, Huaihai Institute of Technology, 59 Cangwu Road, Xinpu 222005, China; Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, Huaihai Institute of Technology, Lianyungang 222005, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Huaihai Institute of Technology, Lianyungang 222005, China. Electronic address: pskgx@yahoo.com.cn.
Saikun
S
Pan
Jiangsu Marine Resources Development Research Institute, Lianyungang, Jiangsu 222005, China; School of Marine Science and Technology, Huaihai Institute of Technology, 59 Cangwu Road, Xinpu 222005, China; Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, Huaihai Institute of Technology, Lianyungang 222005, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Huaihai Institute of Technology, Lianyungang 222005, China.
Shujun
S
Wang
School of Marine Science and Technology, Huaihai Institute of Technology, 59 Cangwu Road, Xinpu 222005, China.
Lingling
L
Jing
Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, 1 Qianhu Houcun, Zhongshanmen Wai, Nanjing 210014, China.
Dongrui
D
Yao
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification*; D002850:Chromatography, Gel; D020128:Inhibitory Concentration 50; D008970:Molecular Weight; D009842:Oligopeptides / Q000737:chemistry / Q000302:isolation & purification; D010447:Peptide Hydrolases / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification*; D011492:Protein Hydrolysates / Q000737:chemistry / Q000302:isolation & purification*; D044445:Ulva / Q000737:chemistry*
D000806:Angiotensin-Converting Enzyme Inhibitors; D009842:Oligopeptides; D010940:Plant Proteins; D011492:Protein Hydrolysates; D010447:Peptide Hydrolases
ACE-inhibitory peptide; Enteromorpha clathrata; Protein hydrolysate; Purification and characterisation