Deciphering the adaptation strategies of Desulfovibrio piezophilus to hydrostatic pressure through metabolic and transcriptional analyses.
Desulfovibrio piezophilus strain C1TLV30(T) is a mesophilic piezophilic sulfate-reducer isolated from Wood Falls at 1700 m depth in the Mediterranean Sea. In this study, we analysed the effect of the hydrostatic pressure on this deep-sea living bacterium at the physiologic and transcriptomic levels. Our results showed that lactate oxidation and energy metabolism were affected by the hydrostatic pressure. Especially, acetyl-CoA oxidation pathway and energy conservation through hydrogen and formate recycling would be more important when the hydrostatic pressure is above (26 MPa) than below (0.1 MPa) the optimal one (10 MPa). This work underlines also the role of the amino acid glutamate as a piezolyte for the Desulfovibrio genus. The transcriptomic analysis revealed 146 differentially expressed genes emphasizing energy production and conversion, amino acid transport and metabolism and cell motility and signal transduction mechanisms as hydrostatic pressure responding processes. This dataset allowed us to identify a sequence motif upstream of a subset of differentially expressed genes as putative pressure-dependent regulatory element.
Aix-Marseille Université, Université du Sud Toulon-Var, IRD, CNRS/INSU, MIO, UM110, Marseille, Cedex 09, 13288, France.|Aix-Marseille Université, CNRS, LCB-UMR7283, Marseille, France.
Amira
A
Amrani
Inserm, U1090; TGML/TAGC, Marseille, F-13009, France.|Aix-Marseille Université, UMR_S 1090; TGML/TAGC, Marseille, F-13007, France.
Jacques
J
van Helden
Inserm, U1090; TGML/TAGC, Marseille, F-13009, France.|Aix-Marseille Université, UMR_S 1090; TGML/TAGC, Marseille, F-13007, France.
Aurélie
A
Bergon
Service de Microscopie Electronique, IBDML, Marseille, Cedex 09, 13288, France.
Aicha
A
Aouane
Aix-Marseille Université, Université du Sud Toulon-Var, IRD, CNRS/INSU, MIO, UM110, Marseille, Cedex 09, 13288, France.
Wajdi
W
Ben Hania
Aix-Marseille Université, Université du Sud Toulon-Var, IRD, CNRS/INSU, MIO, UM110, Marseille, Cedex 09, 13288, France.
Christian
C
Tamburini
Inserm, U1090; TGML/TAGC, Marseille, F-13009, France.|Aix-Marseille Université, UMR_S 1090; TGML/TAGC, Marseille, F-13007, France.
Béatrice
B
Loriod
Inserm, U1090; TGML/TAGC, Marseille, F-13009, France.|Aix-Marseille Université, UMR_S 1090; TGML/TAGC, Marseille, F-13007, France.
Jean
J
Imbert
Aix-Marseille Université, Université du Sud Toulon-Var, IRD, CNRS/INSU, MIO, UM110, Marseille, Cedex 09, 13288, France.
Bernard
B
Ollivier
Aix-Marseille Université, Université du Sud Toulon-Var, IRD, CNRS/INSU, MIO, UM110, Marseille, Cedex 09, 13288, France.
Nathalie
N
Pradel
Aix-Marseille Université, CNRS, LCB-UMR7283, Marseille, France.
Alain
A
Dolla
D000105:Acetyl Coenzyme A / Q000378:metabolism; D000222:Adaptation, Physiological; D003901:Desulfovibrio / Q000502:physiology*; D004734:Energy Metabolism; D005561:Formates / Q000378:metabolism; D020869:Gene Expression Profiling; D018698:Glutamic Acid / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism; D006874:Hydrostatic Pressure; D007773:Lactates / Q000378:metabolism; D008522:Mediterranean Sea; D055432:Metabolomics; D010084:Oxidation-Reduction; D013312:Stress, Physiological
D005561:Formates; D007773:Lactates; D018698:Glutamic Acid; D000105:Acetyl Coenzyme A; D006859:Hydrogen