Evaluation of Presto(plus) assay and LightMix kit Trichomonas vaginalis assay for detection of Trichomonas vaginalis in dry vaginal swabs.
This is an evaluation study of the Presto(plus) Assay for T. vaginalis by comparing to the TIB MOLBIOL LightMix Kit Trichomonas vaginalis Assay using 615 dry collected vaginal and rectal swabs. Discordant samples were analyzed by the Qiagen® Microbial DNA qPCR for TV Assay. Both assays showed comparable performances (McNemar p>0.05).
VU University Medical Centre, Department of Medical Microbiology & Infection Control, Laboratory of Immunogenetics, Amsterdam, The Netherlands; Institute for Public Health Genomics (IPHG), Department of Genetics and Cell Biology, Research School GROW (School for Oncology & Developmental Biology), Faculty of Health, Medicine & Life Sciences, University of Maastricht, Maastricht, The Netherlands.
Dewi J
DJ
de Waaij
VU University Medical Centre, Department of Medical Microbiology & Infection Control, Laboratory of Immunogenetics, Amsterdam, The Netherlands. Electronic address: s.ouburg@vumc.nl.
Sander
S
Ouburg
VU University Medical Centre, Department of Medical Microbiology & Infection Control, Laboratory of Immunogenetics, Amsterdam, The Netherlands; Institute for Public Health Genomics (IPHG), Department of Genetics and Cell Biology, Research School GROW (School for Oncology & Developmental Biology), Faculty of Health, Medicine & Life Sciences, University of Maastricht, Maastricht, The Netherlands.
Jan Henk
JH
Dubbink
Anova Health Institute, Johannesburg and Tzaneen, South Africa; Department of Medical Microbiology, University of Maastricht, Maastricht, The Netherlands; Department of Microbiology, University of Pretoria, Pretoria, South Africa.
Remco P H
RPH
Peters
VU University Medical Centre, Department of Medical Microbiology & Infection Control, Laboratory of Immunogenetics, Amsterdam, The Netherlands; Institute for Public Health Genomics (IPHG), Department of Genetics and Cell Biology, Research School GROW (School for Oncology & Developmental Biology), Faculty of Health, Medicine & Life Sciences, University of Maastricht, Maastricht, The Netherlands.
Servaas A
SA
Morré
D005260:Female; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012007:Rectum / Q000469:parasitology; D012680:Sensitivity and Specificity; D014247:Trichomonas Vaginitis / Q000175:diagnosis* / Q000469:parasitology; D014246:Trichomonas vaginalis / Q000302:isolation & purification*; D014621:Vagina / Q000469:parasitology*; D014626:Vaginal Smears
D011933:Reagent Kits, Diagnostic
Presto(plus); Real-time PCR; TMB LightMix kit Trichomonas vaginalis; Trichomonas vaginalis