Simultaneous quantification of vitamin E, γ-oryzanols and xanthophylls from rice bran essences extracted by supercritical CO2.
Since the nutrition value of rice is diminished during rice processing, technology that can preserve and sustain functional compounds is necessary. In this study, supercritical carbon dioxide (SC-CO2) extraction was optimized for operational conditions (time, temperature, pressure and modifier) to extract vitamin E, γ-oryzanols and xanthophylls from rice bran. The simultaneous quantification of the compounds was developed using high-performance liquid chromatography with diode array and fluorescence detectors. Central composite design and respond surface methodology were applied to achieve optimum extraction conditions. The optimized conditions were 60min, 43°C, 5420psi with 10% ethanol as a modifier. Pigmented rice bran extracts contained greater amounts of functional phytochemicals than non-pigmented rice bran extracts (0.68, 1410, and non-detectable μg/g compared with 16.65, 2480, and 0.10μg/g of vitamin E, γ-oryzanols and xanthophylls in pigmented and non-pigmented ones, respectively). SC-CO2 extraction with modifier would be promising for preparation of phytochemical essences for therapeutic purpose.
Rice and Cereal Chemistry Research Laboratory, Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Electronic address: phumon.s@cmu.ac.th.
Phumon
P
Sookwong
Faculty of Science, Energy and Environment, King Mongkut's University of Technology North Bangkok, Rayong Campus, Rayong 21120, Thailand. Electronic address: panawansut@gmail.com.
Panawan
P
Suttiarporn
Rice and Cereal Chemistry Research Laboratory, Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Electronic address: pittayaporn.boonta@gmail.com.
Pittayaporn
P
Boontakham
Rice and Cereal Chemistry Research Laboratory, Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Electronic address: pattawat_apsc@hotmail.com.
Pattawat
P
Seekhow
Division of Marine Product Technology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, Thailand. Electronic address: suteewang@gmail.com.
Sutee
S
Wangtueai
Rice and Cereal Chemistry Research Laboratory, Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; Center of Excellence for Innovation in Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. Electronic address: sugunya.m@cmu.ac.th.
Sugunya
S
Mahatheeranont
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D025924:Chromatography, Supercritical Fluid; D000431:Ethanol; D009822:Oils, Volatile; D012275:Oryza / Q000737:chemistry*; D010666:Phenylpropionates / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D013696:Temperature; D014810:Vitamin E / Q000032:analysis*; D024341:Xanthophylls / Q000032:analysis*
D009822:Oils, Volatile; D010666:Phenylpropionates; D010936:Plant Extracts; D024341:Xanthophylls; D014810:Vitamin E; D000431:Ethanol; C013172:gamma-oryzanol
Lutein (PubChem CID: 5281243); Rice bran oil; Supercritical carbon dioxide extraction; Vitamin E; Vitamin E (PubChem CID: 5281243); Xanthophylls; γ-Oryzanols; γ-Oryzanols (PubChem CID: 5282164)