Reaction Mechanism of a New Glycosyltrehalose-producing Enzyme Isolated from the Hyperthermophilic Archaeum, Sulfolobus solfataricus KM1.
An amylolytic activity, which converts soluble starch to α,α-trehalose (trehalose), was found in the cell homogenate of the hyperthermophilic, acidophilic archaeum Sulfolobus solfataricus KM1. Two enzymes, a glycosyltransferase and an amylase, which are essential for this activity, were purified to homogeneity. A glycosyltransferase catalyzed the conversion of maltooligosaccharides to glycosyltrehaloses. Based on a detailed analysis of the reaction products, kinetic parameters, and an experiment using (3)H-labeled substrates, it was verified that glycosyltransferase transferred an oligomer segment of maltooligosaccharide to the Cl-OH position of glucose, located at the reducing end of the maltooligosaccharide, to produce a glycosyltrehalose having an α-1,1 linkage. The reaction appears to be intramolecular. Nine strains of the Sulfolobaceae family were found to have glycosyltransferases.
a Applied Bioresearch Center , Kirin Brewery Co., Ltd. , 3 Mivaharacho, Takasakishi, Gunma 370-12 , Japan.
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Kato
a Applied Bioresearch Center , Kirin Brewery Co., Ltd. , 3 Mivaharacho, Takasakishi, Gunma 370-12 , Japan.
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Miura
a Applied Bioresearch Center , Kirin Brewery Co., Ltd. , 3 Mivaharacho, Takasakishi, Gunma 370-12 , Japan.
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Kettoku
a Applied Bioresearch Center , Kirin Brewery Co., Ltd. , 3 Mivaharacho, Takasakishi, Gunma 370-12 , Japan.|b Pharmaceutical Research Laboratories , Kirin Brewery Co., Ltd ., 3 Mivaharacho, Takasakishi, Gunma 370-12 , Japan.
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Shindo
a Applied Bioresearch Center , Kirin Brewery Co., Ltd. , 3 Mivaharacho, Takasakishi, Gunma 370-12 , Japan.|c Central Laboratories for Key Technology , Kirin Brewery Co., Ltd. , 1-13-5 Fukuura, Kanazawaku, Yokohamashi, Kanagawa 236 , Japan.
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Iwamatsu
a Applied Bioresearch Center , Kirin Brewery Co., Ltd. , 3 Mivaharacho, Takasakishi, Gunma 370-12 , Japan.
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Kobayashi
Acidianus brierleyi; Sulfolobus solfataricus; glycosyltransferase; glycosyltrehalose; trehalose