A Walkthrough of Nonlinear Capacitance Measurement of Outer Hair Cells.
Nonlinear capacitance (NLC) measures are often used as surrogate measures of outer hair cell (OHC) electromotility (eM), since the two are commonly thought to share many biophysical features. The measurement of NLC is simpler than direct measurements of eM and, therefore, many investigators have adopted it. A standard patch-clamp hardware configuration is sufficient for recording NLC, given the proper software interface. Thus, the approach is cost effective. We use the software jClamp since it is tailored to capacitance measurement. Here we detail steps that we use to measure NLC. The walk through includes isolation of guinea pig OHCs, building voltage commands, recording, and analysis.
Department of Surgery, Yale University School of Medicine, 208062, New Haven, CT, 06510, USA.
Lei
L
Song
Department of Surgery, Yale University School of Medicine, 208062, New Haven, CT, 06510, USA. joseph.santos-sacchi@yale.edu.|Department of Neurobiology, Yale University School of Medicine, New Haven, CT, 06510, USA. joseph.santos-sacchi@yale.edu.|Department of Physiology, Yale University School of Medicine, New Haven, CT, 06510, USA. joseph.santos-sacchi@yale.edu.
Joseph
J
Santos-Sacchi
D000818:Animals; D025802:Electric Capacitance; D006168:Guinea Pigs; D018072:Hair Cells, Auditory, Outer / Q000502:physiology*; D018408:Patch-Clamp Techniques; D012984:Software
Cochlea; Electromotility; Inner ear; Nonlinear capacitance; Outer hair cells; Prestin