Nonstructural protein 11 (nsp11) of porcine reproductive and respiratory syndrome virus (PRRSV) promotes PRRSV infection in MARC-145 cells.
BACKGROUND
Porcine reproductive and respiratory syndrome virus (PRRSV) induces one of most important devastating disease of swine worldwide, and the current methods poorly control it. Previous studies have indicated that the nonstructural protein 11 (nsp11) of PRRSV may be an important protein for the immune escape of PRRSV.


RESULTS
Here, we firstly explored the effect of over-expression of nsp11 on PRRSV infection and found that over-expression of nsp11 enhanced the PRRSV titers while the small interfering RNA (siRNAs) specifically targeting nsp11 could reduce the PRRSV titers in MARC-145 cells.


CONCLUSIONS
In conclusion, PRRSV nsp11 promotes PRRSV infection in MARC-145 cells and siRNAs targeting nsp11 may be a potential therapeutic strategy to control PRRSV in future.
College of Life Sciences, Henan Normal University, Xinxiang, 453007, China. shixibao@aliyun.com.|Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, 450002, China. shixibao@aliyun.com.
Xibao
X
Shi
College of Life Sciences, Henan Normal University, Xinxiang, 453007, China.|College of Veterinary Medicine and Animal Science, Henan Agricultural University, Zhengzhou, Henan, 450002, China.
Xiaozhuan
X
Zhang
Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, 450002, China.
Yongzhe
Y
Chang
Office of Science & Technology, Chongqing Police College, Chongqing, 401331, China.
Bo
B
Jiang
Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, 450002, China.
Ruiguang
R
Deng
Department of Bioengineering, Zhengzhou University, Zhengzhou, Henan, 450000, 450002, China.
Aiping
A
Wang
Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, 450002, China. zhanggaiping2003@163.com.|College of Veterinary Medicine and Animal Science, Henan Agricultural University, Zhengzhou, Henan, 450002, China. zhanggaiping2003@163.com.
Gaiping
G
Zhang
D000818:Animals; D002460:Cell Line; D004722:Endoribonucleases / Q000378:metabolism; D015870:Gene Expression; D018390:Gene Targeting; D057809:HEK293 Cells; D006801:Humans; D019318:Porcine Reproductive and Respiratory Syndrome / Q000628:therapy / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics / Q000502:physiology*; D034741:RNA, Small Interfering; D013552:Swine; D014162:Transfection; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000502:physiology*; D014779:Virus Replication
D034741:RNA, Small Interfering; D017361:Viral Nonstructural Proteins; D004722:Endoribonucleases; C000657925:nidoviral uridylate-specific endoribonuclease
PRRSV; Small interfering RNA; nsp11