Jeotgalicoccus schoeneichii sp. nov. isolated from exhaust air of a pig barn.
A Gram-staining-positive, non-motile, non-spore-forming, coccus (strain 140805-STR-02T) was isolated from exhaust air of a pig barn on Columbia Blood Agar Base (Oxoid) supplemented with 5 % defibrinated horse blood, Streptococcus selective supplement and 0.5 mg erythromycin l-1. The strains shared high 16S rRNA gene sequence similarity to Jeotgalicoccus pinnipedialis (98.6 %) but only a maximum of 94 % sequence similarity to all other species of the genus Jeotgalicoccus. DNA-DNA hybridisation values between strain 140805-STR-02T and J. pinnipedialis CIP 107946T were 60.3 % (reciprocal, 51.2 %). The quinone system of 140805-STR-02T contained predominantly menaquinone MK-7 and minor amounts of MK-6. The polar lipid profile of strain 140805-STR-02T contained the major compounds diphosphatidylglycerol and phosphatidylglycerol and four unidentified lipids present in minor to moderate amounts. In the polyamine pattern spermidine and spermine were predominant. The fatty acid profile comprising iso-C15 : 0 and anteiso-C15 : 0 as major fatty acids, and was in congruence with those reported for other species of the genus Jeotgalicoccus and thus supported the affiliation of strain 140805-STR-02T to this genus. The results of physiological and biochemical tests allowed a clear phenotypic differentiation of strain 140805-STR-02T from the most closely related species. Strain 140805-STR-02T represents a novel species, for which the names Jeotgalicoccus schoeneichii sp. nov. is proposed, with the type strain 140805-STR-02T (=LMG 29445T=CCM 8667T).
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
Stefanie P
SP
Glaeser
Bundesanstalt für Arbeitschutz und Arbeitsmedizin, D-10317 Berlin, Germany.
Tanita
T
Kleinhagauer
Institut für Mikrobiologie, Veterinärmedizinische Universität, A-1210 Wien, Austria.
Udo
U
Jäckel
Institut für Mikrobiologie, Veterinärmedizinische Universität, A-1210 Wien, Austria.
Kerstin
K
Klug
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
Peter
P
Kämpfer
D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005858:Germany; D006799:Housing, Animal; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011073:Polyamines / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D040141:Staphylococcaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D034421:Sus scrofa; D013552:Swine; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
D004269:DNA, Bacterial; D005227:Fatty Acids; D011073:Polyamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C042455:menaquinone 6