Cyclovirus Vietnam DNA in immunodeficient patients.
Cyclovirus Vietnam (CyCV-VN) is a CyCV detected in 2013 from cerebrospinal fluid (CSF) samples of patients with neurological disorders. Information on prevalence, pathogenesis and disease association of CyCV-VN is still very patchy.



In this study, we have used a PCR assay targeting the Rep gene to investigate the prevalence of CyCV-VN infection in blood and CSF samples of 346 Italian subjects.



Overall, 7% of blood samples were positive for CyCV-VN while the virus was not detected in any of the CSF samples. The prevalence of CyCV-VN was relatively high in HIV positive patients (21%), modest in patients with HBV or HCV infection (6%), and low in transplant recipient patients (2%). Positive patients showed low levels of CyCV-VN viremia. The virus was not detected in serum samples from healthy individuals. Longitudinal analysis of serum samples obtained from selected patients showed a stable or transient presence of circulating CyCV-VN.



The present study is the first to demonstrate CyCV-VN DNA circulation in Italy and to cast light on some biological aspects of this novel virus of men.
Virology Unit, Pisa University Hospital, Pisa, Italy; Department of Biomedical Sciences, Microbiology and Virology Unit, University of Cagliari, Italy; Retrovirus Center and Virology Section, Department of Translational Research, University of Pisa, Italy.
Lisa
L
Macera
Division of Transfusion Medicine and Transplant Biology, Pisa University Hospital, Pisa, Italy.
Daniele
D
Focosi
Virology Unit, Pisa University Hospital, Pisa, Italy.
Maria Linda
ML
Vatteroni
Department of Biomedical Sciences, Microbiology and Virology Unit, University of Cagliari, Italy.
Aldo
A
Manzin
Pasteur Institute-Cenci Bolognetti Foundation, Department of Molecular Medicine, Laboratory of Virology, Sapienza University of Rome, Italy.
Guido
G
Antonelli
Virology Unit, Pisa University Hospital, Pisa, Italy; Retrovirus Center and Virology Section, Department of Translational Research, University of Pisa, Italy.
Mauro
M
Pistello
Virology Unit, Pisa University Hospital, Pisa, Italy. Electronic address: fabrizio.maggi63@gmail.com.
Fabrizio
F
Maggi
D000328:Adult; D000368:Aged; D018136:Circoviridae / Q000235:genetics / Q000302:isolation & purification; D018173:Circoviridae Infections / Q000097:blood / Q000150:complications / Q000821:virology*; D015331:Cohort Studies; D004279:DNA, Viral / Q000097:blood*; D005260:Female; D015658:HIV Infections / Q000150:complications*; D006509:Hepatitis B / Q000150:complications; D006526:Hepatitis C / Q000150:complications; D006801:Humans; D016867:Immunocompromised Host; D007558:Italy; D008297:Male; D008875:Middle Aged
D004279:DNA, Viral
CyCV-VN; Cyclovirus; HIV; Italy; Transplantation