Novel MEKC method for determination of sodium 2-mercaptoethanesulfonate in human plasma with in-capillary derivatization and UV detection.
Sensitive electrophoretic method for determination of total sodium 2-mercaptoethanesulfonate (mesna) in human plasma, based on the stacking with high salt concentration in MEKC and in-capillary derivatization with 2-chloro-1-methyllepidinium tetrafluoroborate followed by UV detection was developed. In the method 0.03molL(-1)pH 7 phosphate buffer with the addition of 0.01molL(-1) SDS, and 10% ACN was used as a BGE. The limit of quantification (LOQ) of the method was 0.5μmolL(-1). Linearity in detector response was observed over the range of 0.5-10μmolL(-1) with the correlation coefficient 0.9971. The intra- and inter-day accuracy (three concentration levels, 5 days, n=3) of the method ranged from 97.2 to 110.0% and from 94.0 to 101.2%, respectively. The novel MEKC method with UV detection proved to be suitable for determination of total mesna in human plasma.
University of Łódź, Faculty of Chemistry, Department of Environmental Chemistry, Poland.
Paulina
P
Furmaniak
University of Łódź, Faculty of Chemistry, Department of Environmental Chemistry, Poland. Electronic address: pakuba@uni.lodz.pl.
Paweł
P
Kubalczyk
University of Łódź, Faculty of Chemistry, Department of Environmental Chemistry, Poland.
Justyna
J
Stachniuk
University of Łódź, Faculty of Chemistry, Department of Environmental Chemistry, Poland. Electronic address: glowar@uni.lodz.pl.
Rafał
R
Głowacki
D020374:Chromatography, Micellar Electrokinetic Capillary / Q000191:economics / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D015080:Mesna / Q000032:analysis* / Q000097:blood*; D020011:Protective Agents / Q000032:analysis* / Q000493:pharmacokinetics; D011806:Quinolinium Compounds / Q000737:chemistry; D012492:Salts / Q000737:chemistry
C000614151:2-chloro-1-methyllepidinium; D020011:Protective Agents; D011806:Quinolinium Compounds; D012492:Salts; D015080:Mesna
2-Chloro-1-methyllepidinium tetrafluoroborate; In-capillary derivatization; Mesna; Plasma; Stacking with high salt concentration in MEKC