Ultrasound-assisted extraction combined with reverse phase-dispersive liquid-liquid micro extraction as a new approach for preconcentration and spectrophotometric determination of total phenol in marine sediments of Chabahar Bay.
In this study, Reverse phase dispersive liquid-liquid micro extraction (RP-DLLME) technique have been successfully developed to preconcentrate trace amount of phenol from sediment samples as a prior step to its derivatization with 4-aminoantipyrine and enhanced determination by UV-Vis spectrophotometry after primary ultrasonic extraction. In this procedure, 50μL 0.7M NaOH solution was chosen as extraction solvent and other factors including pH, extraction time, concentration of 4-aminoantipyrine, type and volume of dispersive solvents were optimized. Under selected conditions, the limit of detection, the linearity range, relative standard deviation and enrichment factor of method were obtained 15μg·kg(-1), 50-1800μg·kg(-1), 4.8% (n=10) and 33, respectively. Finally, using the high sensitivity, low organic solvent consumption and waste generation method, total phenol content in marine sediments from several locations in Chabahar Bay (southeast Iran) was estimated at 55.8-73.2μg·kg(-1).
Department of Marine Chemistry, Faculty of Marine Sciences, Chabahar Maritime University, Chabahar, Iran.
Morteza
M
Ziyaadini
Department of Marine Chemistry, Faculty of Marine Sciences, Chabahar Maritime University, Chabahar, Iran.
Mir Mahdi
MM
Zahedi
Department of Marine Chemistry, Faculty of Marine Sciences, Chabahar Maritime University, Chabahar, Iran.
Saeid
S
Khosravinia
D061006:Bays; D004784:Environmental Monitoring; D019015:Geologic Sediments; D057230:Limit of Detection; D059627:Liquid Phase Microextraction; D019800:Phenol; D010636:Phenols; D012997:Solvents / Q000737:chemistry; D014465:Ultrasonics
D010636:Phenols; D012997:Solvents; D019800:Phenol
4-Aminoantipyrine; Marine sediments; Reverse phase dispersive liquid–liquid micro extraction; Spectrophotometry; Total phenol