Novel electrospun gelatin/oxycellulose nanofibers as a suitable platform for lung disease modeling.
Novel hydrolytically stable gelatin nanofibers modified with sodium or calcium salt of oxycellulose were prepared by electrospinning method. The unique inhibitory effect of these nanofibers against Escherichia coli bacteria was examined by luminometric method. Biocompatibility of these gelatin/oxycellulose nanofibers with eukaryotic cells was tested using human lung adenocarcinoma cell line NCI-H441. Cells firmly adhered to nanofiber surface, as determined by scanning electron microscopy, and no signs of cell dying were detected by fluorescent live/dead assay. We propose that the newly developed gelatin/oxycellulose nanofibers could be used as promising scaffold for lung disease modeling and anti-cancer drug testing.
Institute of Materials Chemistry, Brno University of Technology, Czech Republic. Electronic address: xcsvachova@fch.vutbr.cz.
Veronika
V
Švachová
CEITEC - Central European Institute of Technology, Brno University of Technology, Czech Republic; SCITEG, a.s., Brno, Czech Republic.
Lucy
L
Vojtová
Department of Physical Electronics, Masaryk University, Czech Republic.
David
D
Pavliňák
Institute of Experimental Biology, Masaryk University, Czech Republic.
Libor
L
Vojtek
Department of Histology and Embryology, Masaryk University, Czech Republic; International Clinical Research, St. Anne's University Hospital, Brno, Czech Republic.
Veronika
V
Sedláková
Institute of Experimental Biology, Masaryk University, Czech Republic.
Pavel
P
Hyršl
Department of Physical Electronics, Masaryk University, Czech Republic.
Milan
M
Alberti
Department of Histology and Embryology, Masaryk University, Czech Republic; International Clinical Research, St. Anne's University Hospital, Brno, Czech Republic.
Josef
J
Jaroš
Department of Histology and Embryology, Masaryk University, Czech Republic; International Clinical Research, St. Anne's University Hospital, Brno, Czech Republic.
Aleš
A
Hampl
Institute of Materials Chemistry, Brno University of Technology, Czech Republic; CEITEC - Central European Institute of Technology, Brno University of Technology, Czech Republic; SCITEG, a.s., Brno, Czech Republic.
Josef
J
Jančář
D000230:Adenocarcinoma / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002483:Cellulose, Oxidized / Q000737:chemistry / Q000494:pharmacology; D004926:Escherichia coli / Q000254:growth & development; D005780:Gelatin / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D057139:Nanofibers / Q000737:chemistry*
D002483:Cellulose, Oxidized; D005780:Gelatin
Antibacterial; Electrospinning; Gelatin; Nanofibers; Oxycellulose; Regenerative medicine