High-intensity interval training (swimming) significantly improves the adverse metabolism and comorbidities in diet-induced obese mice.
Controlling obesity and other comorbidities in the population is a challenge in modern society. High-intensity interval training (HIIT) combines short periods of high-intensity exercise with long recovery periods or a low-intensity exercise. The aim was to assess the impact of HIIT in the context of diet-induced obesity in the animal model.



C57BL/6 mice were fed one of the two diets: standard chow (lean group [LE]) or a high-fat diet (obese group [OB]). After twelve weeks, the animals were divided into non-trained groups (LE-NT and OB-NT) and trained groups (LE-T and OB-T), and began an exercise protocol. For biochemical analysis of inflammatory and lipid profile, we used a colorimetric enzymatic method and an automatic spectrophotometer. One-way ANOVA was used for statistical analysis of the experimental groups with Holm-Sidak post-hoc Test. Two-way ANOVA analyzed the interactions between diet and HIIT protocol.



HIIT leads to significant reductions in body mass, blood glucose, glucose tolerance and hepatic lipid profile in T-groups compared to NT-groups. HIIT was able to reduce plasma levels of inflammatory cytokines. Additionally, HIIT improves the insulin immunodensity in the islets, reduces the adiposity and the hepatic steatosis in the T-groups. HIIT improves beta-oxidation and peroxisome proliferator-activated receptor (PPAR)-alpha and reduces lipogenesis and PPAR-gamma levels in the liver. In skeletal muscle, HIIT improves PPAR-alpha and glucose transporter-4 and reduces PPAR-gamma levels.



HIIT leads to attenuate the adverse effects caused by a chronic ingestion of a high-fat diet.
Laboratory of Morphometry, Metabolism and Cardiovascular Disease, Biomedical Center, Institute of Biology, State University of Rio de Janeiro, RJ, Brazil - mandarim@uerj.br.
Victor F
VF
Motta
Marcia B
MB
Aguila
Carlos A
CA
Mandarim-DE-Lacerda
D050154:Adiposity; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D015897:Comorbidity; D000072696:High-Intensity Interval Training; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D008099:Liver; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese / Q000378:metabolism*; D009765:Obesity / Q000209:etiology* / Q000378:metabolism; D044343:Overnutrition; D013550:Swimming
D001786:Blood Glucose; D007328:Insulin