Novel plasmid conferring kanamycin and tetracycline resistance in the turkey-derived Campylobacter jejuni strain 11601MD.
In Campylobacter spp., resistance to the antimicrobials kanamycin and tetracycline is frequently associated with plasmid-borne genes. However, relatively few plasmids of Campylobacter jejuni have been fully characterized to date. A novel plasmid (p11601MD; 44,095nt) harboring tet(O) was identified in C. jejuni strain 11601MD, which was isolated from the jejunum of a turkey produced conventionally in North Carolina. Analysis of the p11601MD sequence revealed the presence of a high-GC content cassette with four genes that included tet(O) and a putative aminoglycoside transferase gene (aphA-3) highly similar to kanamycin resistance determinants. Several genes putatively involved in conjugative transfer were also identified on the plasmid. These findings will contribute to a better understanding of the distribution of potentially self-mobilizing plasmids harboring antibiotic resistance determinants in Campylobacter spp. from turkeys and other sources.
Department of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, Raleigh, NC 27695, USA.
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MD
Crespo
AgResearch Limited Grasslands Research Centre, Palmerston North, New Zealand; Riddet Institute, Massey University, Palmerston North 4442, New Zealand.
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E
Altermann
Department of Biological Sciences, North Carolina State University, Raleigh, NC 27695, USA.
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J
Olson
USDA-ARS, Western Regional Research Center, 800 Buchanan Street, Albany, CA 94710, USA.
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WG
Miller
Department of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, Raleigh, NC 27695, USA.
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K
Chandrashekhar
Department of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, Raleigh, NC 27695, USA. Electronic address: skathar@ncsu.edu.
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S
Kathariou
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D001482:Base Composition; D001483:Base Sequence; D016123:Campylobacter jejuni / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D002352:Carrier Proteins / Q000235:genetics*; D003227:Conjugation, Genetic / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D007583:Jejunum / Q000382:microbiology; D007612:Kanamycin / Q000494:pharmacology*; D019868:Kanamycin Kinase / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D009657:North Carolina; D010957:Plasmids / Q000235:genetics*; D011201:Poultry Diseases / Q000382:microbiology; D017422:Sequence Analysis, DNA; D013752:Tetracycline / Q000494:pharmacology*; D013753:Tetracycline Resistance / Q000235:genetics*; D014422:Turkeys / Q000382:microbiology
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D002352:Carrier Proteins; D004269:DNA, Bacterial; C083139:Tet O resistance protein, Bacteria; D007612:Kanamycin; D019868:Kanamycin Kinase; D013752:Tetracycline
Antibiotic resistance; C. jejuni; Campylobacter; Kanamycin resistance; Plasmid; Tetracycline resistance; aphA-3; tet(O)