Optical waveguide lightmode spectroscopy technique-based immunosensor development for aflatoxin B1 determination in spice paprika samples.
Optical waveguide lightmode spectroscopy (OWLS) technique has been applied to label-free detection of aflatoxin B1 in a competitive immunoassay format, with the aim to compare the analytical goodness of the developed OWLS immunosenor with HPLC and enzyme-linked immunosorbent assay (ELISA) methods for the detection of aflatoxin in spice paprika matrix. We have also assessed applicability of the QuEChERS method prior to ELISA measurements, and the results were compared to those obtained by traditional solvent extraction followed by immunoaffinity clean-up. The AFB1 content of sixty commercial spice paprika samples from different countries were measured with the developed and optimized OWLS immunosensor. Comparing the results from the indirect immunosensor to that obtained by HPLC or ELISA provided excellent correlation (with regression coefficients above 0.94) indicating that the competitive OWLS immunosensor has a potential for quick determination of aflatoxin B1 in paprika samples.
Food Science Research Institute, National Agricultural Research and Innovation Centre, Herman Ottó út 15, H-1022 Budapest, Hungary. Electronic address: k.baranyi@cfri.hu.
Krisztina
K
Majer-Baranyi
Food Science Research Institute, National Agricultural Research and Innovation Centre, Herman Ottó út 15, H-1022 Budapest, Hungary. Electronic address: zs.zalan@cfri.hu.
Zsolt
Z
Zalán
Agro-Environmental Research Institute, National Agricultural Research and Innovation Centre, Herman Ottó út 15, H-1022 Budapest, Hungary. Electronic address: m.mortl@cfri.hu.
Mária
M
Mörtl
Agro-Environmental Research Institute, National Agricultural Research and Innovation Centre, Herman Ottó út 15, H-1022 Budapest, Hungary. Electronic address: j.juracsek@cfri.hu.
Judit
J
Juracsek
MicroVacuum Ltd., Kerékgyártó u. 10, H-1147 Budapest, Hungary. Electronic address: istvan.szendro@microvacuum.com.
István
I
Szendrő
Agro-Environmental Research Institute, National Agricultural Research and Innovation Centre, Herman Ottó út 15, H-1022 Budapest, Hungary. Electronic address: a.szekacs@cfri.hu.
András
A
Székács
Food Science Research Institute, National Agricultural Research and Innovation Centre, Herman Ottó út 15, H-1022 Budapest, Hungary. Electronic address: n.adanyi@cfri.hu.
Nóra
N
Adányi
D016604:Aflatoxin B1 / Q000032:analysis / Q000737:chemistry*; D002212:Capsicum / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D007118:Immunoassay / Q000379:methods*; D013057:Spectrum Analysis / Q000379:methods*; D017365:Spices / Q000032:analysis*
D016604:Aflatoxin B1
Aflatoxin B1; ELISA; Immunosensor; OWLS; Spice paprika