Magnetite/graphene oxide nano-composite for enhancement of hydrogen production from gelatinaceous wastewater.
The effect of substrate to inoculum (So/Xo) ratio and supplementation of magnetite/graphene oxide (MGO) nano-composite material on hydrogen production from gelatinaceous wastewater via dark fermentation process was investigated. Results demonstrated that optimum So/Xo ratio of 1.0gCOD/gVSS achieved maximal hydrogen yield (HY) of 79.2±11.9mL H2/gCOD removed. Supplementation of anaerobes with 100mg/L MGO promoted HY up to 112.4±10.5mL H2/gCOD removed. Moreover, the degradation efficiency of carbohydrates, proteins and lipids was improved to 80.8±7.6, 34.4±2.3 and 31.4±2.2%, respectively. Acetate (HAc) and butyrate (HBu) concentrations increased from 102±6.8 to 125.3±6.3 and from 31.1±1.5 to 48.8±3.5mg/gVSS, respectively. However, propionate (HPr) concentration dropped from 35.9±2.7 to 15±1.3mg/gVSS. Hydrogenase enzyme activity increased 9-folds and the anaerobes elongated from ca. 1.8-2.9 to ca. 2.5-5.1μm with MGO addition. Moreover, Proteobacteria, Firmicutes, Clostridia and Bacilli were detected with the batches supplemented with MGO.
Department of Chemistry, Faculty of Science, Alexandria University, P.O. Box 426, Alexandria 21321, Egypt. Electronic address: ama_mostafa@ymail.com.
Alsayed
A
Mostafa
Department of Chemistry, Faculty of Science, Alexandria University, P.O. Box 426, Alexandria 21321, Egypt.
Ali
A
El-Dissouky
Department of Chemistry, Faculty of Science, Alexandria University, P.O. Box 426, Alexandria 21321, Egypt.
Amal
A
Fawzy
Environmental Engineering Department, Egypt-Japan University of Science and Technology (E-JUST), P.O. Box 179, New Borg El Arab City, 21934 Alexandria, Egypt.
Ahmed
A
Farghaly
Irstea, UR OPAALE, 17 av. de Cucillé, CS 64427, F-35044 Rennes, France.
Pascal
P
Peu
Irstea, UR OPAALE, 17 av. de Cucillé, CS 64427, F-35044 Rennes, France.
Patrick
P
Dabert
Irstea, UR OPAALE, 17 av. de Cucillé, CS 64427, F-35044 Rennes, France.
Sophie
S
Le Roux
Environmental Engineering Department, Egypt-Japan University of Science and Technology (E-JUST), P.O. Box 179, New Borg El Arab City, 21934 Alexandria, Egypt.
Ahmed
A
Tawfik
D000085:Acetates / Q000378:metabolism; D001421:Bacteria, Anaerobic / Q000235:genetics / Q000378:metabolism; D056804:Biofuels; D057919:Biological Oxygen Demand Analysis; D002087:Butyrates / Q000378:metabolism; D050260:Carbohydrate Metabolism; D005285:Fermentation; D052203:Ferrosoferric Oxide / Q000737:chemistry* / Q000378:metabolism; D006108:Graphite / Q000737:chemistry* / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism*; D050356:Lipid Metabolism; D059013:Microbial Consortia / Q000235:genetics / Q000502:physiology; D053761:Nanocomposites / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D014865:Waste Disposal, Fluid / Q000295:instrumentation / Q000379:methods; D062065:Wastewater / Q000737:chemistry*
D000085:Acetates; D056804:Biofuels; D002087:Butyrates; D052203:Ferrosoferric Oxide; D006108:Graphite; D006859:Hydrogen; D010087:Oxides; D011506:Proteins; D062065:Wastewater
Hydrogenase enzyme; Metabolite products; Microbial community analysis; Protein rich wastewater