FoxO1 antagonist suppresses autophagy and lipid droplet growth in adipocytes.
Obesity and related metabolic disorders constitute one of the most pressing heath concerns worldwide. Increased adiposity is linked to autophagy upregulation in adipose tissues. However, it is unknown how autophagy is upregulated and contributes to aberrant adiposity. Here we show a FoxO1-autophagy-FSP27 axis that regulates adipogenesis and lipid droplet (LD) growth in adipocytes. Adipocyte differentiation was associated with upregulation of autophagy and fat specific protein 27 (FSP27), a key regulator of adipocyte maturation and expansion by promoting LD formation and growth. However, FoxO1 specific inhibitor AS1842856 potently suppressed autophagy, FSP27 expression, and adipocyte differentiation. In terminally differentiated adipocytes, AS1842856 significantly reduced FSP27 level and LD size, which was recapitulated by autophagy inhibitors (bafilomycin-A1 and leupeptin, BL). Similarly, AS1842856 and BL dampened autophagy activity and FSP27 expression in explant cultures of white adipose tissue. To our knowledge, this is the first study addressing FoxO1 in the regulation of adipose autophagy, shedding light on the mechanism of increased autophagy and adiposity in obese individuals. Given that adipogenesis and adipocyte expansion contribute to aberrant adiposity, targeting the FoxO1-autophagy-FSP27 axis may lead to new anti-obesity options.
a Department of Human Nutrition, Foods, and Exercise , Fralin Life Science Institute, College of Agriculture and Life Science, Virginia Tech , Blacksburg , VA , USA.
Longhua
L
Liu
a Department of Human Nutrition, Foods, and Exercise , Fralin Life Science Institute, College of Agriculture and Life Science, Virginia Tech , Blacksburg , VA , USA.
Louise D
LD
Zheng
a Department of Human Nutrition, Foods, and Exercise , Fralin Life Science Institute, College of Agriculture and Life Science, Virginia Tech , Blacksburg , VA , USA.
Peng
P
Zou
a Department of Human Nutrition, Foods, and Exercise , Fralin Life Science Institute, College of Agriculture and Life Science, Virginia Tech , Blacksburg , VA , USA.
Joseph
J
Brooke
a Department of Human Nutrition, Foods, and Exercise , Fralin Life Science Institute, College of Agriculture and Life Science, Virginia Tech , Blacksburg , VA , USA.
Cayleen
C
Smith
b Department of Biochemistry , Yong Loo Lin School of Medicine, National University of Singapore , Singapore.
Yun Chau
YC
Long
a Department of Human Nutrition, Foods, and Exercise , Fralin Life Science Institute, College of Agriculture and Life Science, Virginia Tech , Blacksburg , VA , USA.|c Department of Health Promotion, Social & Behavioral Health , College of Public Health, University of Nebraska Medical Center , Omaha , NE , USA.
Fabio A
FA
Almeida
a Department of Human Nutrition, Foods, and Exercise , Fralin Life Science Institute, College of Agriculture and Life Science, Virginia Tech , Blacksburg , VA , USA.
Dongmin
D
Liu
a Department of Human Nutrition, Foods, and Exercise , Fralin Life Science Institute, College of Agriculture and Life Science, Virginia Tech , Blacksburg , VA , USA.
Zhiyong
Z
Cheng
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D050156:Adipogenesis / Q000187:drug effects; D052436:Adipose Tissue, White / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D001343:Autophagy / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D015536:Down-Regulation / Q000187:drug effects; D000071161:Forkhead Box Protein O1 / Q000037:antagonists & inhibitors* / Q000378:metabolism; D066292:Lipid Droplets / Q000187:drug effects / Q000378:metabolism*; D018942:Macrolides / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011506:Proteins / Q000378:metabolism; D015363:Quinolones / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects
C553926:5-amino-7-(cyclohexylamino)-1-ethyl-6-fluoro-4-oxo-1,4-dihydroquinoline-3-carboxylic acid; D000071161:Forkhead Box Protein O1; C467200:Foxo1 protein, mouse; D018942:Macrolides; D011506:Proteins; D015363:Quinolones; C525805:fat-specific protein 27, mouse; C040929:bafilomycin A1
FSP27; adipocyte expansion; adipogenesis; autophagy; FoxO1; lipid droplet