Pyrazinamide Is Active against Mycobacterium tuberculosis Cultures at Neutral pH and Low Temperature.
For the past decades, an acidic pH has been used to render Mycobacterium tuberculosis susceptible to pyrazinamide for in vitro testing. Here, we show that at the standard breakpoint concentration and reduced culture temperatures, pyrazinamide (PZA) is active against tuberculosis (TB) at neutral pH. This finding should help unravel the mechanism of action of PZA and allow drug susceptibility testing (DST) methods to be optimized.
Royal Tropical Institute (KIT), KIT Biomedical Research, Amsterdam, The Netherlands adenhertog@gmail.com r.anthony@planet.nl.
Alice L
AL
den Hertog
Royal Tropical Institute (KIT), KIT Biomedical Research, Amsterdam, The Netherlands.
Sandra
S
Menting
Becton Dickinson Diagnostic Systems, Sparks, Maryland, USA.
Richard
R
Pfeltz
Becton Dickinson Diagnostic Systems, Sparks, Maryland, USA.
Matthew
M
Warns
Becton Dickinson Diagnostic Systems, Sparks, Maryland, USA.
Salman H
SH
Siddiqi
Royal Tropical Institute (KIT), KIT Biomedical Research, Amsterdam, The Netherlands adenhertog@gmail.com r.anthony@planet.nl.
Richard M
RM
Anthony
D000995:Antitubercular Agents / Q000494:pharmacology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008826:Microbial Sensitivity Tests / Q000379:methods; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D011718:Pyrazinamide / Q000494:pharmacology*; D013696:Temperature; D014376:Tuberculosis / Q000188:drug therapy*
D000995:Antitubercular Agents; D011718:Pyrazinamide