Aqueous Two Phase System Assisted Self-Assembled PLGA Microparticles.
Here, we produce poly(lactide-co-glycolide) (PLGA) based microparticles with varying morphologies, and temperature responsive properties utilizing a Pluronic F127/dextran aqueous two-phase system (ATPS) assisted self-assembly. The PLGA polymer, when emulsified in Pluronic F127/dextran ATPS, forms unique microparticle structures due to ATPS guided-self assembly. Depending on the PLGA concentration, the particles either formed a core-shell or a composite microparticle structure. The microparticles facilitate the simultaneous incorporation of both hydrophobic and hydrophilic molecules, due to their amphiphilic macromolecule composition. Further, due to the lower critical solution temperature (LCST) properties of Pluronic F127, the particles exhibit temperature responsiveness. The ATPS based microparticle formation demonstrated in this study, serves as a novel platform for PLGA/polymer based tunable micro/nano particle and polymersome development. The unique properties may be useful in applications such as theranostics, synthesis of complex structure particles, bioreaction/mineralization at the two-phase interface, and bioseparations.
University of Michigan-Dearborn, Department of Mechanical Engineering, 4901 Evergreen Road, Dearborn, MI 48128, USA.
Nitish
N
Yeredla
Macromolecular Science and Engineering Program, University of Michigan Ann Arbor, Ann Arbor, MI 48109, USA.|University of Freiburg, Department of Microsystems Engineering Chemistry and Physics of Interfaces, Georges-Koehler-Allee 103, Freiburg, Germany, 79110.
Taisuke
T
Kojima
University of Michigan-Dearborn, Department of Mechanical Engineering, 4901 Evergreen Road, Dearborn, MI 48128, USA.
Yi
Y
Yang
Macromolecular Science and Engineering Program, University of Michigan Ann Arbor, Ann Arbor, MI 48109, USA.|Biomedical Engineering Department, University of Michigan Ann Arbor, Ann Arbor, MI 48109, USA.
Shuichi
S
Takayama
University of Michigan-Dearborn, Department of Mechanical Engineering, 4901 Evergreen Road, Dearborn, MI 48128, USA.
Mathumai
M
Kanapathipillai