Roles of Wnt pathway genes wls, wnt9a, wnt5b, frzb and gpc4 in regulating convergent-extension during zebrafish palate morphogenesis.
The Wnt signaling pathway is crucial for tissue morphogenesis, participating in cellular behavior changes, notably during the process of convergent-extension. Interactions between Wnt-secreting and receiving cells during convergent-extension remain elusive. We investigated the role and genetic interactions of Wnt ligands and their trafficking factors Wls, Gpc4 and Frzb in the context of palate morphogenesis in zebrafish. We describe that the chaperon Wls and its ligands Wnt9a and Wnt5b are expressed in the ectoderm, whereas juxtaposed chondrocytes express Frzb and Gpc4. Using wls, gpc4, frzb, wnt9a and wnt5b mutants, we genetically dissected the Wnt signals operating between secreting ectoderm and receiving chondrocytes. Our analysis delineates that non-canonical Wnt signaling is required for cell intercalation, and that wnt5b and wnt9a are required for palate extension in the anteroposterior and transverse axes, respectively.
Center for Regenerative Medicine, Massachusetts General Hospital, Harvard Medical School, Harvard Stem Cell Institute, Boston, MA 02114, USA.
Lucie
L
Rochard
Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA 94720, USA.
Stefanie D
SD
Monica
Center for Regenerative Medicine, Massachusetts General Hospital, Harvard Medical School, Harvard Stem Cell Institute, Boston, MA 02114, USA.
Irving T C
IT
Ling
Center for Regenerative Medicine, Massachusetts General Hospital, Harvard Medical School, Harvard Stem Cell Institute, Boston, MA 02114, USA.
Yawei
Y
Kong
Department of Embryology, Carnegie Institution for Science, and Department of Biology, Johns Hopkins University, 3520 San Martin Drive, Baltimore, MD 21218, USA.
Sara
S
Roberson
Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA 94720, USA.
Richard
R
Harland
Department of Embryology, Carnegie Institution for Science, and Department of Biology, Johns Hopkins University, 3520 San Martin Drive, Baltimore, MD 21218, USA.
Marnie
M
Halpern
Center for Regenerative Medicine, Massachusetts General Hospital, Harvard Medical School, Harvard Stem Cell Institute, Boston, MA 02114, USA cliao@partners.org.
Eric C
0000-0003-3989-5115
EC
Liao
D000818:Animals; D049109:Cell Proliferation; D048430:Cell Shape; D019902:Chondrocytes / Q000378:metabolism; D004843:Epistasis, Genetic; D009024:Morphogenesis / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D010159:Palate / Q000196:embryology* / Q000378:metabolism*; D010641:Phenotype; D060449:Wnt Signaling Pathway / Q000235:genetics*; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics*; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism
D029961:Zebrafish Proteins
Convergent-extension; Morphogenesis; Palate; Wnt; Zebrabow