A pharmacokinetic study of Isatin in Beagles' bodies.
Isatin are marine active drugs that exert anti-cancer effects, have a cancer-prevention function, and possess many pharmacological activities. The study aimed to examine the pharmacokinetics of a single intravenous injection and oral medication of Isatin given to Beagles. Nine male and nine female Beagles were injected with 30 mg/kg of 2,3-indole quinones. The animals were divided into 3 groups (n=6 per group) and lavaged with a dose of 15, 30 and 60 mg/kg, respectively. Blood samples were collected prior to the medicine delivery (0 h) and 0.083, 0.25, 0.5, 1, 2, 4, 6, 8 and 24 h post-medicine delivery. The blood plasma samples were analyzed using the liquid chromatography-mass spectrometry (MS)/MS method following pretreatment for the protein precipitation. Pharmacokinetics software was applied to calculate relevant pharmacokinetic parameters through the atrioventricular model. The drug concentration in plasma decreased rapidly following the intravenous injection of Isatin. After 8 h, the prototype drugs could not be tested in the plasma and only trace amounts of drugs were tested in one dog, which was considered to be an endogenous drug. Indole quinone was absorbed following lavage into Beagles and peaked in <1 h, and the drug concentration in the plasma decreased rapidly. After 8 h, the prototype drugs could not be tested in the plasma. The elimination of the two drugs in the body had no evident gender differences. In conclusion, Isatin is rapidly absorbed in bodies of Beagles. Within the dose range of 15-60 mg/kg, no linear relationship was observed for the increase in Cmax and AUC0-tvalues with the increased dose.
Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China.
Ailing
A
Ren
Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China.
Benhua
B
Su
The Second People's Hospital of Jinan City, Jinan, Shandong 250001, P.R. China.
Siyong
S
Ye
The Second People's Hospital of Jinan City, Jinan, Shandong 250001, P.R. China.
Xia
X
Wei
Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China.
Zhaoguo
Z
Fang
Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China.
Qian
Q
Wang
Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China.
Jian
J
Zhang
The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China.
Wen
W
Xu
The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China.
Wang
W
Yue
The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China.
Lei
L
Yin
The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China.
Zhantao
Z
Liu
The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China.
Xiaoling
X
Li
Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China.
B O
BO
Ding
2,3-indole quinine; Beagles; intravenous injection; oral medication; pharmacokinetics