Relationship between HHV8 infection markers and insulin sensitivity in ketosis-prone diabetes.
OBJECTIVE
Peripheral tissue resistance to insulin action is a characteristic of type 2 diabetes mellitus (T2DM). It has also been reported that some chronic viral infections can contribute to insulin resistance. Human herpesvirus (HHV)-8 infection has been detected in T2DM patients in previous studies. Our study investigated whether the presence of the virus is associated with insulin resistance in patients with ketosis-prone type 2 diabetes (KPD), as reported with other viruses.


METHODS
A total of 11 insulin-free KPD patients positive (+) and seven patients who were negative (-) for HHV-8 infection were recruited; the latter had KPD that was well controlled (HbA1c=6.2±0.7%). A two-step euglycaemic-hyperinsulinaemic clamp test coupled with deuterated [6,6-2H2]glucose was used to assess insulin sensitivity, non-esterified fatty acid (NEFA) suppression and endogenous glucose production.


RESULTS
In KPD patients, whether HHV-8+ or HHV-8-, there were no differences in NEFA release, endogenous glucose production or insulin sensitivity (M value).


CONCLUSIONS
Asymptomatic HHV-8 infection does not appear to be associated with decreased insulin sensitivity in diabetic patients. These results should now be confirmed in a larger sample population.
Department of Endocrinology and Diabetes, Lariboisière Hospital, University Paris-Diderot Paris-7, 2, rue Ambroise-Paré, 75010 Paris, France; Inserm UMRS 1138, Cordeliers Research Centre, 15, rue de l'École-de-Medicine, 75006 Paris, France. Electronic address: nguewa.jeanlouis@yahoo.fr.
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JL
Nguewa
Centre for biotechnology, Yaoundé University, Yaoundé, Cameroon.
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E
Lontchi-Yimagou
Department of microbiology, Saint-Louis Hospital, University Paris-Diderot Paris-7, 1, avenue Claude-Vellefaux, 75010 Paris, France.
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F
Agbelika
Department of Endocrinology and Diabetes, Lariboisière Hospital, University Paris-Diderot Paris-7, 2, rue Ambroise-Paré, 75010 Paris, France.
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M
AitDjoudi
Department of biochemistry, Saint-Louis Hospital, University Paris-Diderot Paris-7, 1, avenue Claude-Vellefaux, 75010 Paris, France.
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P
Boudou
Department of internal medecine and Endocrinology, Douala General Hospital, PO Box 4856, Douala, Cameroon; Faculty of Medical Sciences, Buea University, Buea, Cameroon.
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S
Choukem
Centre for biotechnology, Yaoundé University, Yaoundé, Cameroon; National Centre for Obesity, Diabetes, Endocrinology and metabolic diseases, Yaoundé Central Hospital, PO Box 87, Yaoundé, Cameroon.
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E
Sobngwi
Department of Endocrinology and Diabetes, Lariboisière Hospital, University Paris-Diderot Paris-7, 2, rue Ambroise-Paré, 75010 Paris, France; Inserm UMRS 1138, Cordeliers Research Centre, 15, rue de l'École-de-Medicine, 75006 Paris, France; Clinical Investigation Centre, Inserm-CIC 9504, Saint-Louis Hospital, 75010 Paris, France.
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JF
Gautier
D000328:Adult; D003924:Diabetes Mellitus, Type 2 / Q000453:epidemiology / Q000821:virology; D016883:Diabetic Ketoacidosis / Q000453:epidemiology / Q000821:virology; D005260:Female; D015309:Glucose Clamp Technique; D006566:Herpesviridae Infections / Q000150:complications / Q000453:epidemiology; D019288:Herpesvirus 8, Human; D006801:Humans; D007333:Insulin Resistance; D008297:Male; D008875:Middle Aged
Human herpesvirus 8; Insulin resistance; Ketosis-prone type 2 diabetes; Type 2 diabetes