Alteration of transbilayer phospholipid compositions is involved in cell adhesion, cell spreading, and focal adhesion formation.
We previously showed that P4-ATPases, ATP10A/ATP8B1, and ATP11A/ATP11C have flippase activities toward phosphatidylcholine (PC), and aminophospholipids [phosphatidylserine (PS) and phosphatidylethanolamine], respectively. Here, we investigate the effect of PC-specific flippases versus aminophospholipid-specific flippases in cell spreading on the extracellular matrix. Expression of PC-flippases, but not PS-flippases, delayed cell adhesion, cell spreading and inhibited formation of focal adhesions. In addition, overexpression of a PS-binding probe that sequesters PS in the cytoplasmic leaflet delayed cell spreading and inhibited formation of focal adhesions. These results suggest that elevation of PC at the cytoplasmic leaflet of the plasma membrane by expression of PC-flippases may reduce the local concentration of PS or phosphoinositides, required for efficient cell adhesion, focal adhesion formation, and cell spreading.
Graduate School of Pharmaceutical Sciences, Kyoto University, Japan.
Rie
R
Miyano
Graduate School of Pharmaceutical Sciences, Kyoto University, Japan.
Takashi
T
Matsumoto
Graduate School of Pharmaceutical Sciences, Kyoto University, Japan.
Hiroyuki
H
Takatsu
Graduate School of Pharmaceutical Sciences, Kyoto University, Japan.
Kazuhisa
K
Nakayama
Graduate School of Pharmaceutical Sciences, Kyoto University, Japan.
Hye-Won
HW
Shin
D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D002448:Cell Adhesion / Q000502:physiology; D022001:Focal Adhesions / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D010743:Phospholipids / Q000235:genetics / Q000378:metabolism*
D018528:ATP-Binding Cassette Transporters; D026901:Membrane Transport Proteins; D010743:Phospholipids; C000588974:ATP11C protein, human; D000251:Adenosine Triphosphatases; C111067:ATP8B1 protein, human; C426648:ATP10A protein, human; C548142:ATP11A protein, human
ATPase; cell spreading; flippase; focal adhesion; lipid bilayer; phospholipid