Differentiating of cross-reactions in patients with latex allergy with the use of ISAC test.
BACKGROUND
Differentiating between cross-reactivity and double sensitization is still a challenging issue in allergology.


OBJECTIVE
To differentiate cross-reactions accompanying latex allergy with the use of the ISAC test.


METHODS
Thirty-nine patients reporting immediate allergic reactions to latex were enrolled into the study (group A). The control group was comprised of 41 patients with allergic diseases not associated with latex (group B) and 20 healthy individuals (group C). Their history was recorded and skin prick tests were performed with latex, airborne and food allergens. Specific IgE against food allergens, latex (k82) and recombined latex allergens were determined. ImmunoCAP ISAC test was performed with 103 molecules.


RESULTS
Sensitization to latex was found by means of skin tests in 16 cases and sIgE against latex was revealed in 12 cases (including 10 positive in both SPT and sIgE). In the ISAC test antibodies against recombined latex allergens were found in 8 patients with rHev b 6 as the most common. All the patients positive for rHev b 1, 5, 6, 8 had allergy or asymptomatic sensitization to food allergens cross-reacting with latex. Some reactions could not have been differentiated due to the lack of allergens in the ISAC test. Others, not related to latex-fruits syndrome were explained by cross-reactivity with other profilins or PR-10 proteins.


CONCLUSIONS
ImmunoCAP ISAC test could be useful in differentiating between cross-reactions and double sensitizations. However, in the case of latex its advantages are limited due to a small panel of allergens.
Department of Allergology, Medical University of Gdansk, Gdansk, Poland.
Marta
M
Chełmińska
Department of Allergology, Medical University of Gdansk, Gdansk, Poland.
Krzysztof
K
Specjalski
MEDI-LITE, Bielsko-Biala, Poland.
Anna
A
Różyło
Faculty of Electronics, Telecommunications and Informatics, Gdansk University of Technology, Gdansk, Poland.
Agata
A
Kołakowska
Department of Allergology, Medical University of Gdansk, Gdansk, Poland.
Ewa
E
Jassem
ISAC test; cross-reactivity; latex allergy