Live Imaging of Border Cell Migration in Drosophila.
Border cells are a cluster of cells that migrate from the anterior tip of the Drosophila egg chamber to the border of the oocyte in stage 9. They serve as a useful model to study collective cell migration in a native tissue environment. Here we describe a protocol for preparing ex vivo egg chamber cultures from transgenic flies expressing fluorescent proteins in the border cells, and using confocal microscopy to take a multi-positional time-lapse movie. We include an image analysis method for tracking border cell cluster dynamics as well as tracking individual cell movements.
Molecular, Cellular and Developmental Biology Department, University of California, Santa Barbara, CA, 93106, USA.
Wei
W
Dai
Molecular, Cellular and Developmental Biology Department, University of California, Santa Barbara, CA, 93106, USA. dmontell@lifesci.ucsb.edu.
Denise J
DJ
Montell
D000818:Animals; D002465:Cell Movement; D058948:Cell Tracking / Q000379:methods*; D004330:Drosophila / Q000166:cytology* / Q000502:physiology*; D015870:Gene Expression; D017930:Genes, Reporter; D018613:Microscopy, Confocal; D009865:Oocytes / Q000502:physiology*; D009866:Oogenesis; D010063:Ovum / Q000502:physiology*; D059008:Time-Lapse Imaging
3D tracking; Border cell; Confocal; Drosophila; Egg chamber; Live imaging