Multiresponse optimization of an extraction procedure of carnosol and rosmarinic and carnosic acids from rosemary.
A green solvent-based optimization for rosmarinic acid (RA), carnosol (COH), and carnosic acid (CA) extraction, the three main antioxidants from rosemary, was performed. The conventional solid-liquid extraction was optimized using a central composite design (CCD) followed by the desirability approach. In the CCD analysis the quantitative effects of extraction time (4.8-55.2min), liquid-to-solid ratio (4.6-21.4mLg(-1)), and ethanol content (44.8-95.2% v/v) were determined for the extracted amount of antioxidants, their concentrations in the extract, and the extraction yield. Samples were analyzed by HPLC and the antioxidants were identified by comparison with pure standard retention times and UV spectra. The desirability function that simultaneously maximizes the antioxidants extraction and their concentrations in the final product was validated. The extraction using a hydroalcoholic solution 70% v/v, at low liquid-to-solid ratio (5mLg(-1)), and after 55-min yielded an antioxidant recovery rate of 89.8%, and a final product 4.75 times richer in the main antioxidants than the raw material.
Instituto de Química, UFG, 74690-900 Goiânia, GO, Brazil.
Gerlon de A R
Gde A
Oliveira
Instituto de Química, UFG, 74690-900 Goiânia, GO, Brazil. Electronic address: elcana@ufg.br.
Anselmo E
AE
de Oliveira
Faculdade de Farmácia, UFG, 74605-220 Goiânia, GO, Brazil.
Edemílson C
EC
da Conceição
Instituto de Química, UFG, 74690-900 Goiânia, GO, Brazil.
Maria I G
MI
Leles
D045784:Abietanes / Q000032:analysis*; D000975:Antioxidants / Q000032:analysis*; D002934:Cinnamates / Q000032:analysis*; D053630:Depsides / Q000032:analysis*; D010936:Plant Extracts / Q000032:analysis*; D011208:Powders / Q000737:chemistry; D027542:Rosmarinus / Q000737:chemistry*; D000097123:Rosmarinic Acid
D045784:Abietanes; D000975:Antioxidants; D002934:Cinnamates; D053630:Depsides; D010936:Plant Extracts; D011208:Powders; D000097123:Rosmarinic Acid; C068623:carnosol; C018381:salvin
Antioxidants; Conventional extraction; Desirability function; RSM; Rosemary