Response of heterogeneous cancer cells on targeted nanoparticles.
Heterogenous cancer cells possess cancer multidrug resistance (MDR) due to their relative quiescence and ABC-transporter expression. Heterogenous cancer cells can be detected by an Rh123 exclusion assay for identifying Rh123lowpopulation. In the present study, we fabricated targeted nanoparticles entrapped with Rh123 (Rh123 NPs) to investigate the effect of these targeted nanoparticles on an Rh123lowpopulation. The Rh123lowpopulation stained by Rh123 NPs exhibited similar heterogeneity to that stained by Rh123. In addition, the ABC-transporters did not contribute to the uptake of Rh123 or Rh123 NPs. Interestingly, ABC-transporters in the Rh123lowpopulation stained by Rh123 were possibly responsible for Rh123 efflux, while Rh123 NPs were not susceptible to ABC-transporters in the Rh123lowpopulation. It is plausible that the synergistic effect of NPs caused a targeted and endocytic effect which promoted the cellular uptake of Rh123 NPs, and the targeted effect played a more important role.
Guangdong Provincial Key laboratory of cancer immunotherapy Research, Guangzhou Key Laboratory of Tumor Immunology Research, Cancer Research Institute, School of Basic Medical Sciences, Southern Medical University. Electronic address: ningmengquan@gmail.com.
Ying
Y
Wang
Department of Ultrasonography, Nanfang Hospital, Southern Medical University.
Yingjia
Y
Li
Guangdong Provincial Key laboratory of cancer immunotherapy Research, Guangzhou Key Laboratory of Tumor Immunology Research, Cancer Research Institute, School of Basic Medical Sciences, Southern Medical University; Department of Ultrasonography, Nanfang Hospital, Southern Medical University.
Dongxiao
D
Wang
Department of Ultrasonography, Nanfang Hospital, Southern Medical University.
Shaofu
S
Hong
Guangdong Provincial Key laboratory of cancer immunotherapy Research, Guangzhou Key Laboratory of Tumor Immunology Research, Cancer Research Institute, School of Basic Medical Sciences, Southern Medical University.
Jianguo
J
Wang
School of Pharmaceutical Science, Southern Medical University.
Baoping
B
Xie
Guangdong Provincial Key laboratory of cancer immunotherapy Research, Guangzhou Key Laboratory of Tumor Immunology Research, Cancer Research Institute, School of Basic Medical Sciences, Southern Medical University.
Qianbing
Q
Zhang
Department of Neurobiology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.
Xingmei
X
Zhang
Guangdong Provincial Key laboratory of cancer immunotherapy Research, Guangzhou Key Laboratory of Tumor Immunology Research, Cancer Research Institute, School of Basic Medical Sciences, Southern Medical University.
Hongfen
H
Shen
Guangdong Provincial Key laboratory of cancer immunotherapy Research, Guangzhou Key Laboratory of Tumor Immunology Research, Cancer Research Institute, School of Basic Medical Sciences, Southern Medical University.
Qian
Q
Xiao
D018528:ATP-Binding Cassette Transporters; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D006801:Humans; D053758:Nanoparticles; D009369:Neoplasms; D020112:Rhodamine 123 / Q000493:pharmacokinetics*
D018528:ATP-Binding Cassette Transporters; D020112:Rhodamine 123
Heterogenous cancer cells; MDR; Rh123; Targeted nanoparticles