Exploration of the regulatory effect of miR-21 on breast cancer cell line proliferation and invasion as well as the downstream target genes.
OBJECTIVE
To study the regulatory effects of miR-21 on breast cancer cell line proliferation and invasion as well as the downstream target genes.


METHODS
Breast cancer cell lines MCF-7 were cultured and transfected with miR-21 mimics and the corresponding negative control mimics (NC mimics), and then MTS kits were used to detect cell viability. Transwell experiment was used to detect cell invasion ability, and fluorescence quantitative PCR was used to detect the expression of proliferation and invasion-related genes in cells.


RESULTS
24 h after transfection of miR-21 mimics and NC mimics, cell OD value and the number of invasive cells of miR-21 group were significantly higher than those of NC group, and mRNA contents of PDCD-4, FasL, PTEN, RhoB, Maspin, TIMP3 and RECK in cells were significantly lower than those of NC group.


CONCLUSIONS
miR-21 can promote the proliferation and invasion of breast cancer cell lines, and its downstream target genes include PDCD-4, FasL, PTEN, RhoB, Maspin, TIMP3 and RECK.
Central Lab, Guizhou Provincial People's Hospital, Guiyang City, Guizhou Province, 550000, China.
Ying
Y
Kuang
Central Lab, Guizhou Provincial People's Hospital, Guiyang City, Guizhou Province, 550000, China. Electronic address: nienyj@hotmail.com.
Ying-Jie
YJ
Nie
Breast cancer; Invasion; MicroRNA; Proliferation