miR-663a inhibits hepatocellular carcinoma cell proliferation and invasion by targeting HMGA2.
Hepatocellular carcinoma (HCC) is a highly aggressive solid malignancy throughout the world. Dysregulation of miRNAs play essential roles in HCC progression via aberrant regulation of cell proliferation, apoptosis, as well as metastasis. miR-663a is a poorly investigated miRNA. Whether miR-663a regulates HCC development remains unknown. The aim of the study was to explore the role of miR-663a in HCC development. To determine the expression level of miR-663a in HCC, we analyzed the data from GSE21362 and TCGA. The results showed that miR-663a was significantly down-regulated in HCC tissue compared with adjacent non-tumor tissue. Gain of function and loss of function assays revealed that miR-663a distinctly inhibited cell proliferation, migration and invasion. Mechanistic investigations demonstrated that miR-663a modulated cell functions through targeting and suppressing high mobility group A2 (HMGA2). In addition, overexpression of HMGA2 remarkably attenuated the tumor repressive effect of miR-663a. Taken together, miR-663a inhibits HCC cell proliferation and motility by targeting HMGA2.
Department of Medical Oncology, Huizhou Municipal Central Hospital of Guangdong Province, 41 North Eling Road, Huizhou, Guangdong Province 516000, PR China. Electronic address: flyhuizhouzhongxin@163.com.
Weizhen
W
Huang
Department of Medical Oncology, Huizhou Municipal Central Hospital of Guangdong Province, 41 North Eling Road, Huizhou, Guangdong Province 516000, PR China. Electronic address: fly_lijun@aliyun.com.
Jun
J
Li
Department of Medical Oncology, Huizhou Municipal Central Hospital of Guangdong Province, 41 North Eling Road, Huizhou, Guangdong Province 516000, PR China. Electronic address: gxh1979@sina.com.
Xiaohong
X
Guo
Department of Biochemistry, University of Missouri, 117 Schweitzer Hall, Columbia, MO 65211, USA. Electronic address: yingchuzhao@163.com.
Yingchu
Y
Zhao
Department of Medical Oncology, Huizhou Municipal Central Hospital of Guangdong Province, 41 North Eling Road, Huizhou, Guangdong Province 516000, PR China. Electronic address: yuanxia71@hotmail.com.
Xia
X
Yuan
D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D025743:HMGA2 Protein / Q000378:metabolism*; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D010641:Phenotype; D015854:Up-Regulation / Q000235:genetics
D025743:HMGA2 Protein; C552243:MIRN663 microRNA, human; D035683:MicroRNAs
HMGA2; Hepatocellular carcinoma; miR-663a