Whole-Genome Comparative Analysis of Two Carbapenem-Resistant ST-258 Klebsiella pneumoniae Strains Isolated during a North-Eastern Ohio Outbreak: Differences within the High Heterogeneity Zones.
Klebsiella pneumoniae has become one of the most dangerous causative agents of hospital infections due to the acquisition of resistance to carbapenems, one of the last resort families of antibiotics. Resistance is usually mediated by carbapenemases coded for by different classes of genes. A prolonged outbreak of carbapenem-resistant K. pneumoniae infections has been recently described in northeastern Ohio. Most strains isolated from patients during this outbreak belong to MLST sequence type 258 (ST258). To understand more about this outbreak two isolates (strains 140 and 677), one of them responsible for a fatal infection, were selected for genome comparison analyses. Whole genome map and sequence comparisons demonstrated that both strains are highly related showing 99% average nucleotide identity. However, the genomes differ at the so-called high heterogeneity zone (HHZ) and other minor regions. This study identifies the potential value of the HHZ as a potential marker for K. pneumoniae clinical and epidemiological studies.
Center for Applied Biotechnology Studies, Department of Biological Science, California State University Fullerton, Fullerton, CA.
María Soledad
MS
Ramirez
Bioscience Division Los Alamos National Laboratory, Los Alamos, NM.
Gang
G
Xie
IMPaM (UBA-CONICET), University of Buenos Aires, Buenos Aires, Argentina.
German M
GM
Traglia
Bioscience Division Los Alamos National Laboratory, Los Alamos, NM.
Shannon L
SL
Johnson
Bioscience Division Los Alamos National Laboratory, Los Alamos, NM.
Karen W
KW
Davenport
Division of Infectious Diseases, University of North Carolina, Chapel Hill, NC.
David
D
van Duin
Center for Applied Biotechnology Studies, Department of Biological Science, California State University Fullerton, Fullerton, CA Department of Biochemistry, University of Oxford, United Kingdom.
Azam
A
Ramazani
Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, OH.
Federico
F
Perez
Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, OH.
Michael R
MR
Jacobs
Department of Biochemistry, University of Oxford, United Kingdom.
David J
DJ
Sherratt
Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, OH Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, OH.
Robert A
RA
Bonomo
IMPaM (UBA-CONICET), University of Buenos Aires, Buenos Aires, Argentina.
Patrick S G
PS
Chain
Center for Applied Biotechnology Studies, Department of Biological Science, California State University Fullerton, Fullerton, CA mtolmasky@fullerton.edu.
Marcelo E
ME
Tolmasky
D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D015780:Carbapenems / Q000627:therapeutic use; D003428:Cross Infection; D004196:Disease Outbreaks; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D018740:Genetic Heterogeneity; D016680:Genome, Bacterial; D006801:Humans; D007710:Klebsiella Infections / Q000188:drug therapy / Q000235:genetics* / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000235:genetics* / Q000472:pathogenicity
D000900:Anti-Bacterial Agents; D015780:Carbapenems
KPC; ST-258; carbapenem; optical map; whole genome mapping