The between-day reproducibility of fasting, satiety-related analytes, in 8 to 11year-old boys.
The aim of the present study was to establish the between-day reproducibility of fasting plasma GLP-17-36, glucagon, leptin, insulin and glucose, in lean and overweight/obese 8-11year-old boys. A within-group study design was utilised wherein the boys attended two study days, separated by 1week, where a fasting fingertip capillary blood sample was obtained. Deming regression, mean difference, Bland-Altman limits of agreement (LOA) and typical imprecision as a percentage coefficient of variation (CV %), were utilised to assess reproducibility between-days. On a group level, Deming regression detected no evidence of systematic or proportional bias between-days for all of the satiety-related analytes however, only glucose and plasma GLP-17-36 displayed low typical and random imprecision. When analysed according to body composition, good reproducibility was maintained for glucose in the overweight/obese boys and for plasma GLP-17-36, in those with lean body mass. The present findings demonstrate that the measurement of glucose and plasma GLP-17-36 by fingertip capillary sampling on a group level, is reproducible between-days, in 8-11year-old boys. Comparison of blood glucose obtained by fingertip capillary sampling can be made between lean and overweight/obese 8-11year-old boys. Presently, the comparison of fasting plasma GLP-17-36 according to body weight is inappropriate due to high imprecision observed in lean boys between-days. The use of fingertip capillary sampling in the measurement of satiety-related analytes has the potential to provide a better understanding of mechanisms that affect appetite and feeding behaviour in children.
Faculty of Health and Life Sciences, Department of Sport, Exercise and Rehabilitation, Northumbria University, Northumberland Building, Newcastle upon Tyne, NE1 8ST, UK. Electronic address: s.allsop@northumbria.ac.uk.
Susan
S
Allsop
Faculty of Health and Life Sciences, Department of Sport, Exercise and Rehabilitation, Northumbria University, Northumberland Building, Newcastle upon Tyne, NE1 8ST, UK.
Penny L S
PL
Rumbold
Faculty of Health and Life Sciences, Department of Sport, Exercise and Rehabilitation, Northumbria University, Northumberland Building, Newcastle upon Tyne, NE1 8ST, UK.
Benjamin P
BP
Green
D015415:Biomarkers / Q000097:blood; D001774:Blood Chemical Analysis; D001786:Blood Glucose / Q000378:metabolism; D001823:Body Composition; D002648:Child; D005215:Fasting / Q000097:blood*; D005385:Fingers; D005934:Glucagon / Q000097:blood; D052216:Glucagon-Like Peptide 1 / Q000097:blood*; D006801:Humans; D007328:Insulin / Q000097:blood; D020738:Leptin / Q000097:blood; D008297:Male; D050177:Overweight / Q000097:blood; D010446:Peptide Fragments / Q000097:blood*; D012044:Regression Analysis; D015203:Reproducibility of Results; D012527:Satiation / Q000502:physiology*
D015415:Biomarkers; D001786:Blood Glucose; D007328:Insulin; D020738:Leptin; D010446:Peptide Fragments; C061970:glucagon-like peptide 1 (7-36); D052216:Glucagon-Like Peptide 1; D005934:Glucagon
GLP-1(7-36); Glucagon; Glucose; Insulin; Leptin; Satiety