Programmed cell death 4 in bacterially-challenged Apostichopus japonicus: Molecular cloning, expression analysis and functional characterization.
Programmed cell death 4 (PDCD4) plays a crucial role in modulating cellular signals, mainly via TOLL cascades during the immune response. In the present study, a novel PDCD4 homologue gene (denoted as AjPDCD4) was cloned from the sea cucumber Apostichopus japonicus using RACE. The full-length AjPDCD4 cDNA comprised a 366bp 5'-UTR, a 418bp 3'-UTR, and a 1353bp open reading frame encoding a 450 amino acid residue protein with two typical MA3 domains. Phylogenetic analysis revealed that AjPDCD4 belonged to the invertebrate PDCD4 family. Spatial expression analysis indicated that AjPDCD4 mRNA transcripts are expressed at a high level in the tentacles and at a low level in muscle compared with coelomocytes. Vibrio splendidus challenge and LPS exposure could both significantly down-regulate AjPDCD4 mRNA expression. More importantly, we found that ultraviolet (UV)-induced ROS production and DNA damage were greatly repressed in AjPDCD4-knockdown coelomocytes. Meanwhile, the expression levels of the NF-kappa B homologue, p105, were synchronously up-regulated in the same conditions. All of these results indicated that AjPDCD4 is involved in modulating DNA damage and ROS production in sea cucumber, perhaps by affecting the TLR pathway.
School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, PR China.
Zhimeng
Z
Lv
School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, PR China. Electronic address: lichenghua@nbu.edu.cn.
Chenghua
C
Li
School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, PR China.
Yina
Y
Shao
School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, PR China.
Weiwei
W
Zhang
School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, PR China.
Zhenhui
Z
Wang
School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, PR China.
Haihong
H
Wang
D000595:Amino Acid Sequence; D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D003001:Cloning, Molecular; D004249:DNA Damage / Q000276:immunology; D055785:Gene Knockdown Techniques; D010802:Phylogeny; D016601:RNA-Binding Proteins / Q000235:genetics / Q000276:immunology*; D017382:Reactive Oxygen Species / Q000276:immunology; D060888:Real-Time Polymerase Chain Reaction; D017385:Sequence Homology; D047528:Stichopus / Q000235:genetics / Q000276:immunology*; D059467:Transcriptome
D051017:Apoptosis Regulatory Proteins; D016601:RNA-Binding Proteins; D017382:Reactive Oxygen Species
Apostichopus japonicus; DNA damage; Programmed cell death 4; ROS production; p105