Circumscribed Capsular Infarct Modeling Using a Photothrombotic Technique.
Recent increase in the prevalence rate of white matter stroke demands specific research in the field. However, the lack of a pertinent animal model for white matter stroke has hampered research investigations. Here, we describe a novel method for creating a circumscribed capsular infarct that minimizes damage to neighboring gray matter structures. We used pre-surgery neural tracing with adeno-associated virus-green fluorescent protein (AAV-GFP) to identify somatotopic organization of the forelimb area within the internal capsule. The adjustment of light intensity based on different optical properties of gray and white matter contributes to selective destruction of white matter with relative preservation of gray matter. Accurate positioning of optical-neural interface enables destruction of entire forelimb area in the internal capsule, which leads to a marked and persistent motor deficit. Thus, this technique produces highly replicable capsular infarct lesions with a persistent motor deficit. The model will be helpful not only to study white matter stroke (WMS) at the behavioral, circuit, and cellular levels, but also to assess its usefulness for development of new therapeutic and rehabilitative interventions.
Department of Biomedical Science and Engineering, Gwangju Institute of Science and Technology.
Hanlim
H
Song
Department of Biomedical Science and Engineering, Gwangju Institute of Science and Technology.
Ji-Young
JY
Park
Department of Biomedical Science and Engineering, Gwangju Institute of Science and Technology.
Hyung-Sun
HS
Kim
Department of Pathology, Chonnam National University Medical School.
Min-Cheol
MC
Lee
Department of Pathology, Chonnam National University Medical School.
Young
Y
Kim
Department of Biomedical Science and Engineering, Gwangju Institute of Science and Technology; Departement of Neurosurgery, Presbyterian Medical Center; hyoungihl@gist.ac.kr.
Hyoung-Ihl
HI
Kim
D000818:Animals; D000229:Dependovirus; D004195:Disease Models, Animal; D005552:Forelimb; D020772:Internal Capsule; D020521:Stroke