l-(+)-Lactic acid production by Lactobacillus rhamnosus B103 from dairy industry waste.
Lactic acid, which can be obtained through fermentation, is an interesting compound because it can be utilized in different fields, such as in the food, pharmaceutical and chemical industries as a bio-based molecule for bio-refinery. In addition, lactic acid has recently gained more interest due to the possibility of manufacturing poly(lactic acid), a green polymer that can replace petroleum-derived plastics and be applied in medicine for the regeneration of tissues and in sutures, repairs and implants. One of the great advantages of fermentation is the possibility of using agribusiness wastes to obtain optically pure lactic acid. The conventional batch process of fermentation has some disadvantages such as inhibition by the substrate or the final product. To avoid these problems, this study was focused on improving the production of lactic acid through different feeding strategies using whey, a residue of agribusiness. The downstream process is a significant bottleneck because cost-effective methods of producing high-purity lactic acid are lacking. Thus, the investigation of different methods for the purification of lactic acid was one of the aims of this work. The pH-stat strategy showed the maximum production of lactic acid of 143.7g/L. Following purification of the lactic acid sample, recovery of reducing sugars and protein and color removal were 0.28%, 100% and 100%, respectively.
UNESP - Univ. Estadual Paulista, Biological Sciences Institute, Department of Biochemistry and Microbiology, Rio Claro, SP, Brazil.
Marcela Piassi
MP
Bernardo
UNESP - Univ. Estadual Paulista, Biological Sciences Institute, Department of Biochemistry and Microbiology, Rio Claro, SP, Brazil.
Luciana Fontes
LF
Coelho
UNESP - Univ. Estadual Paulista, Biological Sciences Institute, Department of Biochemistry and Microbiology, Rio Claro, SP, Brazil.
Daiane Cristina
DC
Sass
UNESP - Univ. Estadual Paulista, Biological Sciences Institute, Department of Biochemistry and Microbiology, Rio Claro, SP, Brazil; Laboratório Associado do IPBEN-Instituto de Pesquisa em Bioenergy da Unesp, Araraquara, Brazil. Electronic address: jconti@rc.unesp.br.
Jonas
J
Contiero
D000327:Adsorption; D061249:Batch Cell Culture Techniques; D001673:Biodegradation, Environmental; D001711:Biotransformation; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D019344:Lactic Acid / Q000096:biosynthesis*; D052201:Lacticaseibacillus rhamnosus / Q000378:metabolism*; D013696:Temperature
D007220:Industrial Waste; D019344:Lactic Acid
Feed strategies; Lactic acid production; Lactobacillus and lactic acid purification