[Three-dimensional parallel collagen scaffold promotes tendon extracellular matrix formation].
To investigate the effects of three-dimensional parallel collagen scaffold on the cell shape, arrangement and extracellular matrix formation of tendon stem cells.



Parallel collagen scaffold was fabricated by unidirectional freezing technique, while random collagen scaffold was fabricated by freeze-drying technique. The effects of two scaffolds on cell shape and extracellular matrix formation were investigated in vitro by seeding tendon stem/progenitor cells and in vivo by ectopic implantation.



Parallel and random collagen scaffolds were produced successfully. Parallel collagen scaffold was more akin to tendon than random collagen scaffold. Tendon stem/progenitor cells were spindle-shaped and unified orientated in parallel collagen scaffold, while cells on random collagen scaffold had disorder orientation. Two weeks after ectopic implantation, cells had nearly the same orientation with the collagen substance. In parallel collagen scaffold, cells had parallel arrangement, and more spindly cells were observed. By contrast, cells in random collagen scaffold were disorder.



Parallel collagen scaffold can induce cells to be in spindly and parallel arrangement, and promote parallel extracellular matrix formation; while random collagen scaffold can induce cells in random arrangement. The results indicate that parallel collagen scaffold is an ideal structure to promote tendon repairing.
Department of Orthopedic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Orthopedics Research Institute of Zhejiang University, Hangzhou 310009, China;Center of Stem Cell and Tissue Engineering, Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Key Laboratory of Stem Cell of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou 310058, China.
Zefeng
Z
Zheng
Department of Orthopedic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Orthopedics Research Institute of Zhejiang University, Hangzhou 310009, China;Center of Stem Cell and Tissue Engineering, Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Key Laboratory of Stem Cell of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou 310058, China.
Weiliang
W
Shen
Department of Orthopedic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Orthopedics Research Institute of Zhejiang University, Hangzhou 310009, China;Center of Stem Cell and Tissue Engineering, Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Key Laboratory of Stem Cell of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou 310058, China.
Huihui
H
Le
Department of Orthopedic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Orthopedics Research Institute of Zhejiang University, Hangzhou 310009, China.
Xuesong
X
Dai
Center of Stem Cell and Tissue Engineering, Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Key Laboratory of Stem Cell of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou 310058, China. hwoy@zju.edu.cn.
Hongwei
H
Ouyang
Department of Orthopedic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Orthopedics Research Institute of Zhejiang University, Hangzhou 310009, China. chenweishan@zju.edu.cn.
Weishan
W
Chen
D003094:Collagen / Q000737:chemistry*; D005109:Extracellular Matrix / Q000502:physiology*; D005612:Freeze Drying; D005615:Freezing; D006801:Humans; D013234:Stem Cells / Q000166:cytology*; D013710:Tendons / Q000166:cytology / Q000254:growth & development*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
D003094:Collagen