Engineering Biomolecular Switches for Dynamic Metabolic Control.
Living organisms have been exploited as production hosts for a large variety of compounds. To improve the efficiency of bioproduction, metabolic pathways in an organism are usually manipulated by various genetic modifications. However, bottlenecks during the conversion of substrate to a desired product may result from cellular regulations at different levels. Dynamic regulation of metabolic pathways according to the need of cultivation process is therefore essential for developing effective bioprocesses, but represents a major challenge in metabolic engineering and synthetic biology. To this end, switchable biomolecules which can sense the intracellular concentrations of metabolites with different response types and dynamic ranges are of great interest. This chapter summarizes recent progress in the development of biomolecular switches and their applications for improvement of bioproduction via dynamic control of metabolic fluxes. Further studies of bioswitches and their applications in industrial strain development are also discussed.
Institute of Bioprocess and Biosystems Engineering, Hamburg University of Technology, Denickestrasse 15, 21073, Hamburg, Germany.
Cheng-Wei
CW
Ma
Institute of Bioprocess and Biosystems Engineering, Hamburg University of Technology, Denickestrasse 15, 21073, Hamburg, Germany.
Li-Bang
LB
Zhou
Institute of Bioprocess and Biosystems Engineering, Hamburg University of Technology, Denickestrasse 15, 21073, Hamburg, Germany. aze@tu-harburg.de.
An-Ping
AP
Zeng
D060847:Metabolic Engineering / Q000379:methods*; D008660:Metabolism / Q000235:genetics*
Biomolecular engineering; Bioswitches; Cellular regulation; Dynamic metabolic control; Strain development