Tyrosine-phosphorylation of the scaffold protein ADAP and its role in T cell signaling.
The Adhesion and Degranulation promoting Adaptor Protein (ADAP) is phosphorylated upon T cell activation and acts as a scaffold for the formation of a signaling complex that integrates molecular interactions between T cell or chemokine receptors, the actin cytoskeleton, and integrin-mediated cellular adhesion and migration.



This article reviews current knowledge of the functions of the adapter protein ADAP in T cell signaling with a focus on the role of individual phosphotyrosine (pY) motifs for SH2 domain mediated interactions. The data presented was obtained from literature searches (PubMed) as well as the authors own research on the topic. Expert commentary: ADAP can be regarded as a paradigmatic example of how tyrosine phosphorylation sites serve as dynamic interaction hubs. Molecular crowding at unstructured and redundant sites (pY595, pY651) is contrasted by more specific interactions enabled by the three-dimensional environment of a particular phosphotyrosine motif (pY571).
a Freie Universität Berlin, Institut für Chemie und Biochemie, Protein Biochemistry Group , Berlin , Germany.|b Mass Spectrometry Group, Leibniz-Institut für Molekulare Pharmakologie , Berlin , Germany.
Benno
B
Kuropka
c Institute of Molecular and Clinical Immunology , Otto-von-Guericke-University , Magdeburg , Germany.|d Department of Immune Control , Helmholtz Center for Infection Research (HZI) , Braunschweig , Germany.
Burkhart
B
Schraven
c Institute of Molecular and Clinical Immunology , Otto-von-Guericke-University , Magdeburg , Germany.
Stefanie
S
Kliche
b Mass Spectrometry Group, Leibniz-Institut für Molekulare Pharmakologie , Berlin , Germany.
Eberhard
E
Krause
a Freie Universität Berlin, Institut für Chemie und Biochemie, Protein Biochemistry Group , Berlin , Germany.
Christian
C
Freund
D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D006801:Humans; D010766:Phosphorylation; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000378:metabolism*; D014443:Tyrosine / Q000737:chemistry / Q000378:metabolism
D048868:Adaptor Proteins, Signal Transducing; C106957:FYB1 protein, human; D014443:Tyrosine
Affinity purification–mass spectrometry; T cell signaling; pulldown experiment; quantitative proteomics; scaffold protein; tyrosine phosphorylation