N-terminal domain of PB1-F2 protein of influenza A virus can fold into amyloid-like oligomers and damage cholesterol and cardiolipid containing membranes.
PB1-F2 protein is a factor of virulence of influenza A viruses which increases the mortality and morbidity associated with infection. Most seasonal H1N1 Influenza A viruses express nowadays a truncated version of PB1-F2. Here we show that truncation of PB1-F2 modified supramolecular organization of the protein in a membrane-mimicking environment. In addition, full-length PB1-F2(1-90) and C-terminal PB1-F2 domain (53-90), efficiently permeabilized various anionic liposomes while N-terminal domain PB1-F2(1-52) only lysed cholesterol and cardiolipin containing lipid bilayers. These findings suggest that the truncation of PB1-F2 may impact the pathogenicity of a given virus strain.
Virologie et Immunologie Moléculaires, UR892, INRA, Paris Saclay University, 78350 Jouy en Josas, France; Laboratoire de Physique Statistique, École Normale Supérieure, 24 rue Lhomond, 75005 Paris, France.
Dalila
D
Ajjaji
Virologie et Immunologie Moléculaires, UR892, INRA, Paris Saclay University, 78350 Jouy en Josas, France.
Charles-Adrien
CA
Richard
Institut de Chimie Moléculaire et des Matériaux d'Orsay, Université Paris-Sud, CNRS, UMR 8182, 91400 Orsay, France.
Sandra
S
Mazerat
Virologie et Immunologie Moléculaires, UR892, INRA, Paris Saclay University, 78350 Jouy en Josas, France. Electronic address: christophe.chevalier@jouy.inra.fr.
Christophe
C
Chevalier
Virologie et Immunologie Moléculaires, UR892, INRA, Paris Saclay University, 78350 Jouy en Josas, France; Nanyang Technological University-Hebrew University of Jerusalem-Ben Gurion University, NEW CREATE, Singapore 138602, Singapore. Electronic address: jasmina.vidic@jouy.inra.fr.
Jasmina
J
Vidic
D000682:Amyloid / Q000737:chemistry*; D001704:Biopolymers / Q000737:chemistry*; D002308:Cardiolipins / Q000032:analysis*; D002462:Cell Membrane / Q000737:chemistry*; D002784:Cholesterol / Q000737:chemistry*; D009980:Influenza A virus / Q000737:chemistry*; D017510:Protein Folding; D014764:Viral Proteins / Q000737:chemistry*
D000682:Amyloid; D001704:Biopolymers; D002308:Cardiolipins; D014764:Viral Proteins; D002784:Cholesterol
Amyloid-like protein structures; Cardiolipin; Cholesterol; Influenza A viruses; PB1-F2-Membrane interaction