Photoprotective Effect of Carpomitra costata Extract against Ultraviolet B-Induced Oxidative Damage in Human Keratinocytes.
Natural marine products show various biological properties such as antiphotoaging, antioxidant, anticancer, and anti-inflammation. This study evaluated the protective effects of the brown alga Carpomitra costata (Stackhouse) Batters (Sporochnaceae) against ultraviolet B (UVB)-provoked damage in human HaCaT keratinocytes. C. costata extract (CCE) effectively reduced superoxide anion, hydroxyl radical, and UVB-stimulated intracellular reactive oxygen species (ROS) levels. CCE also restored the expression and activity of UVB-suppressed antioxidant enzymes. Furthermore, CCE decreased UVB-triggered oxidative damage to cellular components including DNA, protein, and lipid and defended the cells against mitochondrial membrane depolarization-medicated apoptosis. The results of this study indicate that CCE can safeguard human keratinocytes against UVB-induced cellular damage via a potent antioxidant mechanism. CCE may find utility as part of a therapeutic arsenal against the damaging effects of UVB radiation on the skin.
School of Medicine, Jeju National University, Jeju, Republic of Korea.
J
J
Zheng
School of Medicine, Jeju National University, Jeju, Republic of Korea.
S R K Madduma
SR
Hewage
School of Medicine, Jeju National University, Jeju, Republic of Korea.
Mei Jing
MJ
Piao
School of Medicine, Jeju National University, Jeju, Republic of Korea.
Kyoung Ah
KA
Kang
School of Medicine, Jeju National University, Jeju, Republic of Korea.
X
X
Han
School of Medicine, Jeju National University, Jeju, Republic of Korea.
H K
HK
Kang
School of Medicine, Jeju National University, Jeju, Republic of Korea.
E S
ES
Yoo
School of Medicine, Jeju National University, Jeju, Republic of Korea.
Y S
YS
Koh
Department of Chemistry, College of Natural Sciences, Jeju National University, Jeju, Republic of Korea.
N H
NH
Lee
Jeju Biodiversity Research Institute, Jeju Technopark, Jeju, Republic of Korea.
C S
CS
Ko
Jeju Biodiversity Research Institute, Jeju Technopark, Jeju, Republic of Korea.
J C
JC
Lee
Jeju Biodiversity Research Institute, Jeju Technopark, Jeju, Republic of Korea.
Mi Hee
MH
Ko
School of Medicine, Jeju National University, Jeju, Republic of Korea.
Jin Won
JW
Hyuna
D000975:Antioxidants / Q000378:metabolism*; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000528:radiation effects*; D000459:Phaeophyceae / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D015595:Skin Aging / Q000187:drug effects*; D014466:Ultraviolet Rays
D000975:Antioxidants; D010936:Plant Extracts