Optimization of Lipase production from a novel strain Thalassospira permensis M35-15 using Response Surface Methodology.
Lipases can catalyze the hydrolysis of glycerol, esters and long chain fatty acids. A lipase producing isolate M35-15 was screened and identified as Thalassospira permensis using 16S rRNA gene sequence analysis. To our knowledge this is the first report on Thalassospira permensis producing lipases. In this paper the optimization of medium composition for the increase in bacterial lipase was achieved using statistical methods. Firstly the key ingredients were selected by Plackett-Burman experimental design, then the levels of the ingredients were optimized using central composite design of Response Surface Methodology. The predicted optimal lipase activity was 11.49 U under the conditions that medium composition were 5.15 g/l glucose, 11.74 g/l peptone, 6.74 g/l yeast powder and 22.90 g/l olive oil emulsifier.
a School of Marine Science and Technology, Harbin Institute of Technology , Weihai , People's Republic of China.
Wang
W
Kai
a School of Marine Science and Technology, Harbin Institute of Technology , Weihai , People's Republic of China.
Yan
Y
Peisheng
D003470:Culture Media; D004269:DNA, Bacterial / Q000235:genetics; D005990:Glycerol / Q000737:chemistry; D006868:Hydrolysis; D008049:Lipase / Q000096:biosynthesis*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012244:Rhodospirillaceae / Q000145:classification / Q000201:enzymology*; D017422:Sequence Analysis, DNA
D003470:Culture Media; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D008049:Lipase; D005990:Glycerol
Plackett-burman design; Thalassospira sp; central composite design; lipase; optimization