Design of cocktail peptide vaccine against Cytomegalovirus infection.
OBJECTIVE
Human Cytomegalovirus (HCMV) remains a major morbidity and mortality cause in immuno suppressed patients. Therefore, significant effort has been made towards the development of a vaccine. In this study, the expression of the pp65 and gB fusion peptides and Fc domain of mouse IgG2a as a novel delivery system for selective uptake of antigens by antigen-presenting cells (APCs) in Pichia pastoris yeast system were studied.


METHODS
In this study, four immune dominant sequences in pp65 protein and 3 immuno dominant sequences in gB protein were selected according to literature review. Peptide linker -GGGGS- was used for construction of fusion peptide. This fusion peptide was cloned in the pPICZαA expression vector and transfected into P. pastoris host cells.


RESULTS
Dot blot and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) techniques showed that a high level of pp65-gB-Fc fusion peptide was expressed.


CONCLUSIONS
This CMV pp65-gB-Fc fusion peptide could be a promising candidate for the development of a novel peptide vaccine.
Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran.
Samira
S
Tabaei
Department of Medical Biochemistry, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Baratali
B
Mashkani
Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran.
Arezoo
A
Esmaili
Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran.
Reza
R
Karimi
Antimicrobial Resistance Research Center, Avicenna Research Institute, Mashhad University of Medical Science, Mashhad, Iran.
Saeid Amel
SA
Jamehdar
Human cytomegalovirus; Peptide vaccine; Pichia pastoris; pp65