Photothermal combined gene therapy achieved by polyethyleneimine-grafted oxidized mesoporous carbon nanospheres.
Combining controllable photothermal therapy and efficacious gene therapy in a single platform holds great promise in cancer therapy due to the enhanced combined therapeutic effects. Herein, polyethyleneimine-grafted oxidized mesoporous carbon nanospheres (OP) were developed for combined photothermal combined gene therapy in vitro and in vivo. The synthesized OP was characterized to have three dimensional spherical structure with uniformed diameter, ordered mesopores with graphitic domains, high water dispersion with zeta potential of +22 mV, and good biocompatibility. Consequently, OP was exploited as the photothermal convertor with strong NIR absorption and the gene vector via electrostatic interaction, which therefore cannot only deliver the therapeutic gene (pING4) to tumors for gene therapy, but also can eliminate the tumors by photothermal ablation. Moreover, the improved gene therapy accompanied by the NIR photothermally enhanced gene release was also well achieved based on OP. The excellent combined therapeutic effects demonstrated in vitro and in vivo suggested the OP's potential for cancer therapy.
Department of Pharmaceutics, School of Pharmacy, Key Laboratory of Smart Drug Delivery, Ministry of Education, Fudan University, Shanghai 201203, China; Department of Pharmaceutics, School of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China.
Ying
Y
Meng
Department of Pharmaceutics, School of Pharmacy, Key Laboratory of Smart Drug Delivery, Ministry of Education, Fudan University, Shanghai 201203, China.
Shanshan
S
Wang
Department of Pharmaceutics, School of Pharmacy, Key Laboratory of Smart Drug Delivery, Ministry of Education, Fudan University, Shanghai 201203, China.
Chengyi
C
Li
Department of Pharmaceutics, School of Pharmacy, Key Laboratory of Smart Drug Delivery, Ministry of Education, Fudan University, Shanghai 201203, China.
Min
M
Qian
Department of Pharmaceutics, School of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China.
Xueying
X
Yan
Department of Pharmaceutics, School of Pharmacy, Key Laboratory of Smart Drug Delivery, Ministry of Education, Fudan University, Shanghai 201203, China.
Shuangchao
S
Yao
Department of Pharmaceutics, School of Pharmacy, Key Laboratory of Smart Drug Delivery, Ministry of Education, Fudan University, Shanghai 201203, China.
Xiyue
X
Peng
Center of Analysis and Measurement, Fudan University, Shanghai 200433, China. Electronic address: wangyifd@fudan.edu.cn.
Yi
Y
Wang
Department of Pharmaceutics, School of Pharmacy, Key Laboratory of Smart Drug Delivery, Ministry of Education, Fudan University, Shanghai 201203, China. Electronic address: rqhuang@fudan.edu.cn.
Rongqin
R
Huang
D000818:Animals; D001940:Breast / Q000378:metabolism / Q000473:pathology; D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D002244:Carbon / Q000737:chemistry / Q000627:therapeutic use*; D018797:Cell Cycle Proteins / Q000235:genetics; D004247:DNA / Q000008:administration & dosage / Q000235:genetics / Q000627:therapeutic use*; D005260:Female; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D006979:Hyperthermia, Induced / Q000379:methods; D061986:MCF-7 Cells; D008819:Mice, Nude; D054118:Nanospheres / Q000737:chemistry / Q000627:therapeutic use* / Q000648:ultrastructure; D010084:Oxidation-Reduction; D010789:Phototherapy / Q000379:methods; D011094:Polyethyleneimine / Q000737:chemistry / Q000627:therapeutic use*; D025521:Tumor Suppressor Proteins / Q000235:genetics
D018797:Cell Cycle Proteins; D018398:Homeodomain Proteins; C483230:ING4 protein, human; D025521:Tumor Suppressor Proteins; D002244:Carbon; D011094:Polyethyleneimine; D004247:DNA
Combined therapy; Gene therapy; Mesoporous carbon nanospheres; Photothermal therapy