Novel surface antigen based impedimetric immunosensor for detection of Salmonella typhimurium in water and juice samples.
A specific surface antigen, OmpD has been reported first time as a surface biomarker in the development of selective and sensitive immunosensor for detecting Salmonella typhimurium species. The OmpD surface antigen extraction was done from Salmonella typhimurium serovars, under the optimized growth conditions for its expression. Anti-OmpD antibodies were generated and used as detector probe in immunoassay format on graphene-graphene oxide (G-GO) modified screen printed carbon electrodes. The water samples were spiked with standard Salmonella typhimurium cells, and detection was done by measuring the change in impedimetric response of developed immunosensor to know the concentration of serovar Salmonella typhimurium. The developed immunosensor was able to specifically detect S. typhimurium in spiked water and juice samples with a sensitivity upto 10(1)CFUmL(-1), with high selectivity and very low cross-reactivity with other strains. This is the first report on the detection of Salmonella typhimurum species using a specific biomarker, OmpD. The developed technique could be very useful for the detection of nontyphoidal Salmonellosis and is also important from an epidemiological point of view.
CSIR-Institute of Microbial Technology, Sector 39-A, Chandigarh 160036, India.
Ruchi
R
Mutreja
CSIR-Institute of Microbial Technology, Sector 39-A, Chandigarh 160036, India.
Monu
M
Jariyal
CSIR-Institute of Microbial Technology, Sector 39-A, Chandigarh 160036, India.
Preeti
P
Pathania
CSIR-Institute of Microbial Technology, Sector 39-A, Chandigarh 160036, India.
Arunima
A
Sharma
CSIR-Institute of Microbial Technology, Sector 39-A, Chandigarh 160036, India.
D K
DK
Sahoo
CSIR-Institute of Microbial Technology, Sector 39-A, Chandigarh 160036, India. Electronic address: raman@imtech.res.in.
C Raman
CR
Suri
D055910:Antibodies, Immobilized / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation; D058266:Dielectric Spectroscopy / Q000295:instrumentation*; D004867:Equipment Design; D000067030:Fruit and Vegetable Juices / Q000382:microbiology*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation; D057230:Limit of Detection; D010087:Oxides / Q000737:chemistry; D018272:Porins / Q000032:analysis*; D012480:Salmonella Infections / Q000382:microbiology; D012486:Salmonella typhimurium / Q000302:isolation & purification*; D014871:Water Microbiology
D055910:Antibodies, Immobilized; C470933:OmpD protein, Salmonella typhimurium; D010087:Oxides; D018272:Porins; D006108:Graphite
Graphene-graphene oxide Nanocomposite; Immunosensor; Impedimetric; OmpD; Salmonella typhimurium