Cell-specific restoration of stimulus preference after monocular deprivation in the visual cortex.
Monocular deprivation evokes a prominent shift of neuronal responses in the visual cortex toward the open eye, accompanied by functional and structural synaptic rearrangements. This shift is reversible, but it is unknown whether the recovery happens at the level of individual neurons or whether it reflects a population effect. We used ratiometric Ca(2+) imaging to follow the activity of the same excitatory layer 2/3 neurons in the mouse visual cortex over months during repeated episodes of ocular dominance (OD) plasticity. We observed robust shifts toward the open eye in most neurons. Nevertheless, these cells faithfully returned to their pre-deprivation OD during binocular recovery. Moreover, the initial network correlation structure was largely recovered, suggesting that functional connectivity may be regained despite prominent experience-dependent plasticity.
Max Planck Institute of Neurobiology, Am Klopferspitz 18, D-82152 Martinsried, Germany. trose@neuro.mpg.de tobias.bonhoeffer@neuro.mpg.de.
Tobias
T
Rose
Max Planck Institute of Neurobiology, Am Klopferspitz 18, D-82152 Martinsried, Germany.
Juliane
J
Jaepel
Max Planck Institute of Neurobiology, Am Klopferspitz 18, D-82152 Martinsried, Germany.
Mark
M
Hübener
Max Planck Institute of Neurobiology, Am Klopferspitz 18, D-82152 Martinsried, Germany. trose@neuro.mpg.de tobias.bonhoeffer@neuro.mpg.de.
Tobias
T
Bonhoeffer
D000818:Animals; D002118:Calcium / Q000032:analysis; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000235:genetics; D023882:Dominance, Ocular / Q000502:physiology*; D051379:Mice; D059906:Neuroimaging / Q000379:methods; D009474:Neurons / Q000502:physiology; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics; D012683:Sensory Deprivation / Q000502:physiology; D059010:Single-Cell Analysis / Q000379:methods; D014793:Visual Cortex / Q000166:cytology / Q000502:physiology*
D011506:Proteins; C570393:ruby-eye 2 protein, mouse; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D002118:Calcium