Carbon allocation, osmotic adjustment, antioxidant capacity and growth in cotton under long-term soil drought during flowering and boll-forming period.
Responses of plant to drought largely depend on the intensity, duration and developmental stage at which water stress occurs. The purpose of this study was to analyze the dynamic of cotton physiology response to different levels sustained soil water deficit during reproductive growth stage at leaf basis. Three levels of steady-state water regimes [soil relative water content (SRWC) maintained at (75 ± 5)%, (60 ± 5)% and (45 ± 5)%] were imposed when the white flowers had opened on the first fruiting position of the 6-7th fruiting branches (FB6-7), which was the first day post anthesis (i.e. 1 DPA) and lasted to 50 DPA. Results showed decreasing SRWC slowed cotton growth on the base of biomass and leaf area. However, carbon metabolites levels were globally increased under drought despite of notably inhibited photosynthesis throughout the treatment period. Clear diurnal pattern of sucrose and starch concentrations was obtained and sucrose levels were evaluated while starch concentration was reduced with decreasing soil water content during a 24-h cycle. Osmotic adjustment (OA) was observed at most of the sampling dates throughout the drought period. K(+) was the main contributor to osmotic adjustment (OA) at 10 and 24 DPA then turned out to be amino acid at 38 and 50 DPA. The stressed cotton gradually failed to scavenge reactive oxygen species (ROS) with increasing days post anthesis, primarily due to the permanent decrease in SOD activity. Elevated carbohydrates levels suggest cotton growth was more inhibited by other factors than carbon assimilation. OA and antioxidant could be important protective mechanisms against soil water deficit in this species, and transition of these mechanisms was observed with drought intensity and duration increased.
Key Laboratory of Crop Growth Regulation, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, PR China.
Rui
R
Wang
Key Laboratory of Crop Growth Regulation, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, PR China.
Min
M
Gao
Key Laboratory of Crop Growth Regulation, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, PR China.
Shu
S
Ji
Key Laboratory of Crop Growth Regulation, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, PR China.
Shanshan
S
Wang
Key Laboratory of Crop Growth Regulation, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, PR China.
Yali
Y
Meng
Key Laboratory of Crop Growth Regulation, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, PR China. Electronic address: giscott@njau.edu.cn.
Zhiguo
Z
Zhou
D000975:Antioxidants / Q000378:metabolism*; D018533:Biomass; D002241:Carbohydrates / Q000032:analysis; D002244:Carbon / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D002940:Circadian Rhythm; D055864:Droughts; D035264:Flowers / Q000502:physiology*; D003368:Gossypium / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism*; D008315:Malondialdehyde / Q000378:metabolism; D009995:Osmosis; D009195:Peroxidase / Q000378:metabolism; D010788:Photosynthesis; D018515:Plant Leaves / Q000502:physiology; D012987:Soil / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000378:metabolism; D013696:Temperature; D013997:Time Factors; D014867:Water / Q000737:chemistry; D014887:Weather
D000975:Antioxidants; D002241:Carbohydrates; D012987:Soil; D014867:Water; D008315:Malondialdehyde; D002244:Carbon; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase
Antioxidant; Carbon allocation; Cotton; Drought; Osmotic adjustment