Serum microRNA panels as potential biomarkers for early detection of hepatocellular carcinoma on top of HCV infection.
The identification of new high-sensitivity and high-specificity markers for hepatocellular carcinoma (HCC) is essential. We aimed at identifying serum microRNAs (miRNAs) as potential biomarkers for early detection of HCC on top hepatitis C virus (HCV) infection. We investigated serum expression of 13 miRNAs in 384 patients with HCV-related chronic liver disease (192 with HCC, 96 with liver cirrhosis (LC), and 96 with chronic hepatitis C (CHC)) in addition to 96 healthy participants enrolled as a control group. The miRNA expression was performed using real-time quantitative PCR-based SYBR Green custom miRNA arrays. The area under the receiver operating characteristic curve (AUC) was used to evaluate the diagnostic performance of miRNA panels for early detection of HCC. Using miRNA panel of miR-122, miR-885-5p, and miR-29b with alpha fetoprotein (AFP) provided high diagnostic accuracy (AUC = 1) for early detection of HCC in normal population while using miRNA panel of miR-122, miR-885-5p, miR-221, and miR-22 with AFP provided high diagnostic accuracy (AUC = 0.982) for early detection of HCC in LC patients. However, using miRNA panel of miR-22 and miR-199a-3p with AFP provided high diagnostic accuracy (AUC = 0.988) for early detection of HCC in CHC patients. We identified serum miRNA panels that could have a considerable clinical use in early detection of HCC in both normal population and high-risk patients.
Molecular Virology and Immunology Unit, Cancer biology Department, National Cancer Institute, Cairo University, Cairo, Egypt. ncizekri@yahoo.com.
Abdel-Rahman Nabawy
AN
Zekri
Molecular Virology and Immunology Unit, Cancer biology Department, National Cancer Institute, Cairo University, Cairo, Egypt. amira.salah@nci.cu.edu.eg.
Amira Salah El-Din
AS
Youssef
Epidemiology and Biostatistics Unit, Epidemiology and Biostatistics Department, National Cancer Institute, Cairo, Egypt.
Eman Desouky
ED
El-Desouky
Molecular Virology and Immunology Unit, Cancer biology Department, National Cancer Institute, Cairo University, Cairo, Egypt.
Ola Sayed
OS
Ahmed
Molecular Virology and Immunology Unit, Cancer biology Department, National Cancer Institute, Cairo University, Cairo, Egypt.
Mai M
MM
Lotfy
Molecular Virology and Immunology Unit, Cancer biology Department, National Cancer Institute, Cairo University, Cairo, Egypt.
Auhood Abdel-Monem
AA
Nassar
Pathology Department, National Cancer Institute, Cairo University, Cairo, Egypt.
Abeer A
AA
Bahnassey
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics*; D006528:Carcinoma, Hepatocellular / Q000150:complications / Q000175:diagnosis / Q000235:genetics*; D016022:Case-Control Studies; D042241:Early Diagnosis; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D016174:Hepacivirus / Q000502:physiology; D019698:Hepatitis C, Chronic / Q000150:complications / Q000235:genetics* / Q000821:virology; D006801:Humans; D008113:Liver Neoplasms / Q000150:complications / Q000175:diagnosis / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D012372:ROC Curve; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult; D000509:alpha-Fetoproteins / Q000235:genetics
D014408:Biomarkers, Tumor; D035683:MicroRNAs; D000509:alpha-Fetoproteins
Early detection; HCC; HCV; Panel; Serum; miRNAs