Imaging Invasion: Micro-CT imaging of adamantinomatous craniopharyngioma highlights cell type specific spatial relationships of tissue invasion.
Tissue invasion and infiltration by brain tumours poses a clinical challenge, with destruction of structures leading to morbidity. We assessed whether micro-CT could be used to map tumour invasion in adamantinomatous craniopharyngioma (ACP), and whether it could delineate ACPs and their intrinsic components from surrounding tissue.Three anonymised archival frozen ACP samples were fixed, iodinated and imaged using a micro-CT scanner prior to the use of standard histological processing and immunohistochemical techniques.We demonstrate that micro-CT imaging can non-destructively give detailed 3D structural information of tumours in volumes with isotropic voxel sizes of 4-6 microns, which can be correlated with traditional histology and immunohistochemistry.Such information complements classical histology by facilitating virtual slicing of the tissue in any plane and providing unique detail of the three dimensional relationships of tissue compartments.
Institute of Child Health, University College London, London, UK.|Great Ormond Street Hospital, London, UK.
John R
JR
Apps
Institute of Child Health, University College London, London, UK. Ciaran.Hutchinson@gosh.nhs.uk.|Great Ormond Street Hospital, London, UK. Ciaran.Hutchinson@gosh.nhs.uk.
J Ciaran
JC
Hutchinson
Great Ormond Street Hospital, London, UK.
Owen J
OJ
Arthurs
Great Ormond Street Hospital, London, UK.
Alex
A
Virasami
Department of Histopathology, Royal Victoria Infirmary, Newcastle, England.
Abhijit
A
Joshi
μVIS X-ray Imaging Centre, Faculty of Engineering and the Environment, University of Southampton, Southampton, UK.
Berit
B
Zeller-Plumhoff
Institute of Child Health, University College London, London, UK.
Dale
D
Moulding
Institute of Child Health, University College London, London, UK.|Great Ormond Street Hospital, London, UK.
Thomas S
TS
Jacques
Institute of Child Health, University College London, London, UK.|Great Ormond Street Hospital, London, UK.
Neil J
NJ
Sebire
Institute of Child Health, University College London, London, UK.
Juan Pedro
JP
Martinez-Barbera
D003397:Craniopharyngioma / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000473:pathology; D015925:Cryopreservation; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006801:Humans; D021621:Imaging, Three-Dimensional; D007150:Immunohistochemistry; D009361:Neoplasm Invasiveness; D016612:Paraffin Embedding; D010911:Pituitary Neoplasms / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000473:pathology; D055114:X-Ray Microtomography; D051176:beta Catenin / Q000378:metabolism
C495270:CTNNB1 protein, human; D005904:Glial Fibrillary Acidic Protein; D051176:beta Catenin