In Vivo Metal Ion Imaging Using Fluorescent Sensors.
In vivo imaging in living animals provides the ability to monitor alterations of signaling molecules, ions, and other biological components during various life stages and in disease. The data gained from in vivo imaging can be used for biological discovery or to determine elements of disease progression and can inform the development and translation of therapeutics. Herein, we present theories behind small-molecule, fluorescent, metal ion sensors as well as the methods for their successful application to in vivo metal ion imaging, including ex vivo validation.
Department of Radiology, Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, USA. gvandebittner@mgh.harvard.edu.
Genevieve C
GC
Van de Bittner
Laboratory of Medicinal & Pharmaceutical Chemistry, Gifu Pharmaceutical University, Gifu, Japan.
Tasuku
T
Hirayama
D000818:Animals; D015374:Biosensing Techniques; D003300:Copper / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000008:administration & dosage* / Q000737:chemistry; D051379:Mice; D057054:Molecular Imaging; D051598:Whole Body Imaging / Q000379:methods*
D005456:Fluorescent Dyes; D003300:Copper
Copper; Fluorescence; IVIS spectrum; In vivo imaging; Ion; Metal; Near-infrared; Wilson’s disease