Caveolin-1 Mediates Low-Intensity Ultrasound-Induced Apoptosis via Downregulation of Signal Transducer and Activator of Transcription 3 Phosphorylation in Laryngeal Carcinoma Cells.
Low-intensity ultrasound therapy has been found to be a potential tool in the management of malignant tumors in recent years. However, the molecular mechanism underlying low-intensity ultrasound-induced apoptosis is still not clear. In this study, we investigated the effects of low-intensity ultrasound-induced apoptosis in HEp-2 cells. We found that low-intensity ultrasound significantly induced apoptosis, and the expression level of caveolin-1 (Cav-1) was dramatically increased after ultrasound treatment of HEp-2 cells. After inhibiting the expression level of Cav-1 using siRNA transfection, we found that the cellular apoptosis induced by low-intensity ultrasound was significantly suppressed. In addition, inhibition of Cav-1 expression promoted phosphorylation of signal transducer and activator of transcription 3 (STAT3), suggesting that the STAT3 signaling pathway was involved in low-intensity ultrasound-induced apoptosis via Cav-1 regulation. Our results indicate that Cav-1/STAT3 signaling pathway may mediate low-intensity ultrasound-induced apoptosis, and this technology could potentially be used clinically for the treatment of cancers.
Medical Ultrasonic Engineering Department, Institute of Biomedical Engineering Chinese Academy of Medical Sciences, Tianjin, China.
Qingsheng
Q
Ye
Department of Otolaryngology Head and Neck Surgery, China-Japan Union Hospital, Jilin University, Changchun, China.
Cuida
C
Meng
Key Laboratory of Radiobiology (Ministry of Health), School of Public Health, Jilin University, Changchun, China.
Yannan
Y
Shen
Medical Ultrasonic Engineering Department, Institute of Biomedical Engineering Chinese Academy of Medical Sciences, Tianjin, China.
Jianjun
J
Ji
Medical Ultrasonic Engineering Department, Institute of Biomedical Engineering Chinese Academy of Medical Sciences, Tianjin, China.
Xiaochun
X
Wang
Medical Ultrasonic Engineering Department, Institute of Biomedical Engineering Chinese Academy of Medical Sciences, Tianjin, China.
Sheng
S
Zhou
Key Laboratory of Radiobiology (Ministry of Health), School of Public Health, Jilin University, Changchun, China. Electronic address: jiall@jlu.edu.cn.
Lili
L
Jia
Medical Ultrasonic Engineering Department, Institute of Biomedical Engineering Chinese Academy of Medical Sciences, Tianjin, China. Electronic address: yeyeqingsheng@163.com.
Yanqun
Y
Wang
D017209:Apoptosis / Q000235:genetics / Q000502:physiology*; D015153:Blotting, Western; D051242:Caveolin 1 / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation / Q000235:genetics / Q000502:physiology*; D005434:Flow Cytometry; D006801:Humans; D007822:Laryngeal Neoplasms / Q000628:therapy*; D010766:Phosphorylation; D050796:STAT3 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D014464:Ultrasonic Therapy / Q000009:adverse effects* / Q000379:methods
D051242:Caveolin 1; D050796:STAT3 Transcription Factor
Apoptosis; Caveolin-1; Low-intensity ultrasound; STAT3