OsLBD3-7 Overexpression Induced Adaxially Rolled Leaves in Rice.
Appropriate leaf rolling enhances erect-leaf habits and photosynthetic efficiency, which consequently improves grain yield. Here, we reported the novel lateral organ boundaries domain (LBD) gene OsLBD3-7, which is involved in the regulation of leaf rolling. OsLBD3-7 works as a transcription activator and its protein is located on the plasma membrane and in the nucleus. Overexpression of OsLBD3-7 leads to narrow and adaxially rolled leaves. Microscopy of flag leaf cross-sections indicated that overexpression of OsLBD3-7 led to a decrease in both bulliform cell size and number. Transcriptional analysis showed that key genes that had been reported to be negative regulators of bulliform cell development were up-regulated in transgenic plants. These results indicated that OsLBD3-7 might acts as an upstream regulatory gene of bulliform cell development to regulate leaf rolling, which will give more insights on the leaf rolling regulation mechanism.
Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Cong
C
Li
Shanghai Key Laboratory of Protected Horticultural Technology, Forestry and Fruit Tree Research Institute, Shanghai Academy of Agricultural Sciences (SAAS), Shanghai, 201403, China.
Xiaohua
X
Zou
Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Chunyu
C
Zhang
Jinan Licheng No.2 High School, Jinan, Shandong province, 250104, China.
Qinghao
Q
Shao
Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Jun
J
Liu
Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Bin
B
Liu
Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Hongyu
H
Li
Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Tao
http://orcid.org/0000-0002-5641-8161
T
Zhao
D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D010788:Photosynthesis / Q000235:genetics / Q000502:physiology; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism
D010940:Plant Proteins