Cell structure imaging with bright and homogeneous nanometric light source.
Label-free optical nano-imaging of dendritic structures and intracellular granules in biological cells is demonstrated using a bright and homogeneous nanometric light source. The optical nanometric light source is excited using a focused electron beam. A zinc oxide (ZnO) luminescent thin film was fabricated by atomic layer deposition (ALD) to produce the nanoscale light source. The ZnO film formed by ALD emitted the bright, homogeneous light, unlike that deposited by another method. The dendritic structures of label-free macrophage receptor with collagenous structure-expressing CHO cells were clearly visualized below the diffraction limit. The inner fiber structure was observed with 120 nm spatial resolution. Because the bright homogeneous emission from the ZnO film suppresses the background noise, the signal-to-noise ratio (SNR) for the imaging results was greater than 10. The ALD method helps achieve an electron beam excitation assisted microscope with high spatial resolution and high SNR.
Graduate School of Science and Technology, Shizuoka University, 3-5-1 Johoku, Naka, Hamamatsu, 432-8561, Japan.
Masahiro
M
Fukuta
Research Institute of Electronics, Shizuoka University, 3-5-1 Johoku, Naka, Hamamatsu, 432-8561, Japan.|CREST, Japan Science and Technology Agency, 4-1-8 Honmachi, Kawaguchi, Saitama, 332-0012, Japan.
Atsushi
A
Ono
Research Institute of Electronics, Shizuoka University, 3-5-1 Johoku, Naka, Hamamatsu, 432-8561, Japan.
Yasunori
Y
Nawa
Research Institute of Electronics, Shizuoka University, 3-5-1 Johoku, Naka, Hamamatsu, 432-8561, Japan.|CREST, Japan Science and Technology Agency, 4-1-8 Honmachi, Kawaguchi, Saitama, 332-0012, Japan.
Wataru
W
Inami
CREST, Japan Science and Technology Agency, 4-1-8 Honmachi, Kawaguchi, Saitama, 332-0012, Japan.
Lin
L
Shen
Research Institute of Electronics, Shizuoka University, 3-5-1 Johoku, Naka, Hamamatsu, 432-8561, Japan.|CREST, Japan Science and Technology Agency, 4-1-8 Honmachi, Kawaguchi, Saitama, 332-0012, Japan.
Yoshimasa
Y
Kawata
CREST, Japan Science and Technology Agency, 4-1-8 Honmachi, Kawaguchi, Saitama, 332-0012, Japan.|Photon Medical Research Center, Hamamatsu University School of Medicine, 1-20-1 Hondayama, Higashi, Hamamatsu, 431-3192, Japan.
Susumu
S
Terekawa
D000818:Animals; D056444:Axonemal Dyneins / Q000648:ultrastructure; D016466:CHO Cells; D003412:Cricetulus; D004867:Equipment Design; D006046:Gold; D053768:Metal Nanoparticles; D008853:Microscopy / Q000295:instrumentation; D061848:Optical Imaging / Q000295:instrumentation; D010775:Photic Stimulation / Q000295:instrumentation; D011971:Receptors, Immunologic / Q000378:metabolism; D015034:Zinc Oxide
D011971:Receptors, Immunologic; D006046:Gold; D056444:Axonemal Dyneins; C430745:DNAH9 protein, human; D015034:Zinc Oxide
bio-imaging; cathodoluminescence; super-resolution microscope