Daunomycin-loaded superparamagnetic iron oxide nanoparticles: Preparation, magnetic targeting, cell cytotoxicity, and protein delivery research.
The clinical use of daunomycin is restricted by dose-dependent toxicity and low specificity against cancer cells. In the present study, modified superparamagnetic iron oxide nanoparticles were employed to load daunomycin and the drug-loaded nanospheres exhibited satisfactory size and smart pH-responsive release. The cellular uptake efficiency, targeted cell accumulation, and cell cytotoxicity experimental results proved that the superparamagnetic iron oxide nanoparticle-loading process brings high drug targeting without decreasing the cytotoxicity of daunomycin. Moreover, a new concern for the evaluation of nanophase drug delivery's effects was considered, with monitoring the interactions between human serum albumin and the drug-loaded nanospheres. Results from the multispectroscopic techniques and molecular modeling calculation elucidate that the drug delivery has detectable deleterious effects on the frame conformation of protein, which may affect its physiological function.
School of Chemistry and Chemical Engineering, Guangdong Pharmaceutical University, Guangzhou, PR China.
Min-Chao
MC
Liu
School of Chemistry and Chemical Engineering, Guangdong Pharmaceutical University, Guangzhou, PR China.
Shu-Fang
SF
Jin
School of Basic, Guangdong Pharmaceutical University, Guangzhou, PR China.
Min
M
Zheng
School of Chemistry and Chemical Engineering, Guangdong Pharmaceutical University, Guangzhou, PR China.
Yan
Y
Wang
School of Chemistry and Chemical Engineering, Guangdong Pharmaceutical University, Guangzhou, PR China.
Peng-Liang
PL
Zhao
School of Chemistry and Chemical Engineering, Guangdong Pharmaceutical University, Guangzhou, PR China.
Ding-Tong
DT
Tang
School of Chemistry and Chemical Engineering, Guangdong Pharmaceutical University, Guangzhou, PR China.
Jiong
J
Chen
School of Chemistry and Chemical Engineering, Guangdong Pharmaceutical University, Guangzhou, PR China.
Jia-Qi
JQ
Lin
School of Chemistry and Chemical Engineering, Guangdong Pharmaceutical University, Guangzhou, PR China.
Xia-Hong
XH
Wang
School of Chemistry and Chemical Engineering, Guangdong Pharmaceutical University, Guangzhou, PR China zhaoping666@163.com.
Ping
P
Zhao
D002470:Cell Survival; D003630:Daunorubicin / Q000494:pharmacology*; D004317:Doxorubicin / Q000737:chemistry; D016503:Drug Delivery Systems; D065546:Drug Liberation; D005290:Ferric Compounds / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D062105:Molecular Docking Simulation; D054118:Nanospheres / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry
D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; D012709:Serum Albumin; C000499:ferric oxide; D004317:Doxorubicin; D003630:Daunorubicin
Daunomycin; carrier protein; conformation; interaction; superparamagnetic iron oxide nanoparticle