The prevalence of aminoglycoside-modifying enzyme and virulence genes among enterococci with high-level aminoglycoside resistance in Inner Mongolia, China.
This study highlights the prevalence of aminoglycoside-modifying enzyme genes and virulence determinants among clinical enterococci with high-level aminoglycoside resistance in Inner Mongolia, China. Screening for high-level aminoglycoside resistance against 117 enterococcal clinical isolates was performed using the agar-screening method. Out of the 117 enterococcal isolates, 46 were selected for further detection and determination of the distribution of aminoglycoside-modifying enzyme-encoding genes and virulence determinants using polymerase chain reaction -based methods. Enterococcus faecium and Enterococcus faecalis were identified as the species of greatest clinical importance. The aac(6')-Ie-aph(2″)-Ia and ant(6')-Ia genes were found to be the most common aminoglycoside-modifying enzyme genes among high-level gentamicin resistance and high-level streptomycin resistance isolates, respectively. Moreover, gelE was the most common virulence gene among high-level aminoglycoside resistance isolates. Compared to Enterococcus faecium, Enterococcus faecalis harbored multiple virulence determinants. The results further indicated no correlation between aminoglycoside-modifying enzyme gene profiles and the distribution of virulence genes among the enterococcal isolates with high-level gentamicin resistance or high-level streptomycin resistance evaluated in our study.
The First Affiliated Hospital, Baotou Medical College, Baotou, China.
Haiying
H
Niu
The Second Affiliated Hospital, Baotou Medical College, Baotou, China.
Hui
H
Yu
The First Affiliated Hospital, Baotou Medical College, Baotou, China.
Tangping
T
Hu
The First Affiliated Hospital, Baotou Medical College, Baotou, China.
Gailin
G
Tian
The First Affiliated Hospital, Baotou Medical College, Baotou, China.
Lixia
L
Zhang
The First Affiliated Hospital, Baotou Medical College, Baotou, China.
Xiang
X
Guo
Department of Pathophysiology, Baotou Medical College, Baotou, China. Electronic address: btyxyhh@163.com.
Hai
H
Hu
The First Affiliated Hospital, Baotou Medical College, Baotou, China. Electronic address: wang.zhanli@hotmail.com.
Zhanli
Z
Wang
D000617:Aminoglycosides / Q000378:metabolism* / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002681:China / Q000453:epidemiology; D024881:Drug Resistance, Bacterial; D016983:Enterococcus / Q000187:drug effects* / Q000302:isolation & purification / Q000502:physiology*; D005260:Female; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D005798:Genes, Bacterial; D016908:Gram-Positive Bacterial Infections / Q000453:epidemiology / Q000382:microbiology; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D015995:Prevalence; D014774:Virulence / Q000235:genetics
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents
Aminoglycoside-modifying enzyme gene; Enterococcus; High-level aminoglycoside resistance; Virulence determinant