Analysis of the genetic diversity of Candida isolates obtained from diabetic patients and kidney transplant recipients.
Yeasts of the genus Candida have high genetic variability and are the most common opportunistic pathogenic fungi in humans. In this study, we evaluated the genetic diversity among 120 isolates of Candida spp. obtained from diabetic patients, kidney transplant recipients and patients without any immune deficiencies from Paraná state, Brazil. The analysis was performed using the ITS1-5.8S-ITS2 region and a partial sequence of 28S rDNA. In the phylogenetic analysis, we observed a consistent separation of the species C. albicans, C. dubliniensis, C. glabrata, C. tropicalis, C. parapsilosis, C. metapsilosis and C. orthopsilosis, however with low intraspecific variability. In the analysis of the C. albicans species, two clades were formed. Clade A included the largest number of isolates (91.2%) and the majority of isolates from GenBank (71.4%). The phylogenetic analysis showed low intraspecific genetic diversity, and the genetic polymorphisms between C. albicans isolates were similar to genetic divergence found in other studies performed with isolates from Brazil. This low genetic diversity of isolates can be explained by the geographic proximity of the patients evaluated. It was observed that yeast colonisation was highest in renal transplant recipients and diabetic patients and that C. albicans was the species most frequently isolated.
Universidade Paranaense, Universidade Paranaense, Departamento de Microbiologia, Francisco Beltrão PR , Brasil, Universidade Paranaense, Departamento de Microbiologia, Francisco Beltrão, PR, Brasil.|Universidade Federal do Paraná, Universidade Federal do Paraná, Departamento de Patologia Básica, Curitiba PR , Brasil, Universidade Federal do Paraná, Departamento de Patologia Básica, Curitiba, PR, Brasil.
Volmir Pitt
VP
Benedetti
Universidade Federal do Paraná, Universidade Federal do Paraná, Departamento de Patologia Básica, Curitiba PR , Brasil, Universidade Federal do Paraná, Departamento de Patologia Básica, Curitiba, PR, Brasil.|Universidade Federal do Paraná, Universidade Federal do Paraná, Departamento de Genética, Curitiba PR , Brasil, Universidade Federal do Paraná, Departamento de Genética, Curitiba, PR, Brasil.
Daiani Cristina
DC
Savi
Universidade Federal do Paraná, Universidade Federal do Paraná, Departamento de Genética, Curitiba PR , Brasil, Universidade Federal do Paraná, Departamento de Genética, Curitiba, PR, Brasil.
Rodrigo
R
Aluizio
Universidade Federal do Paraná, Universidade Federal do Paraná, Departamento de Genética, Curitiba PR , Brasil, Universidade Federal do Paraná, Departamento de Genética, Curitiba, PR, Brasil.
Douglas
D
Adamoski
Universidade Federal do Paraná, Universidade Federal do Paraná, Departamento de Genética, Curitiba PR , Brasil, Universidade Federal do Paraná, Departamento de Genética, Curitiba, PR, Brasil.
Vanessa
V
Kava-Cordeiro
Universidade Federal do Paraná, Universidade Federal do Paraná, Departamento de Genética, Curitiba PR , Brasil, Universidade Federal do Paraná, Departamento de Genética, Curitiba, PR, Brasil.
Lygia V
LV
Galli-Terasawa
Universidade Federal do Paraná, Universidade Federal do Paraná, Departamento de Patologia Básica, Curitiba PR , Brasil, Universidade Federal do Paraná, Departamento de Patologia Básica, Curitiba, PR, Brasil.|Universidade Federal do Paraná, Universidade Federal do Paraná, Departamento de Genética, Curitiba PR , Brasil, Universidade Federal do Paraná, Departamento de Genética, Curitiba, PR, Brasil.
Chirlei
C
Glienke
D001938:Brazil / Q000453:epidemiology; D002175:Candida / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D058365:Candidiasis, Invasive / Q000145:classification / Q000453:epidemiology / Q000235:genetics* / Q000382:microbiology; D016022:Case-Control Studies; D004271:DNA, Fungal / Q000032:analysis; D004275:DNA, Ribosomal / Q000235:genetics; D048909:Diabetes Complications; D003920:Diabetes Mellitus / Q000382:microbiology*; D005260:Female; D014644:Genetic Variation; D006801:Humans; D016030:Kidney Transplantation; D008297:Male; D008826:Microbial Sensitivity Tests
D004271:DNA, Fungal; D004275:DNA, Ribosomal