Population structure and antimicrobial profile of Staphylococcus aureus strains associated with bovine mastitis in China.
Staphylococcus aureus is a significant bacterial pathogen associated with bovine mastitis. The aim of the present study was to investigate and characterize of S. aureus strains isolated from the milk of cows suffering from mastitis in the mid-east of China. Among the 200 milk samples analyzed, 58 were positive for S. aureus, of these isolates, 11 isolates were methicillin-resistant Staphylococcus aureus (MRSA). All of the 58 S. aureus strains were classified in agr group I, while seven different sequence type (ST) patterns were identified and among them the most common was ST630 followed by ST188. All of the S. aureus isolates belonging to ST630 were resistant to more than four antimicrobials, and 22.2% of isolates belonging to ST188 were resistant to eight antimicrobials. Interestingly, while strong biofilm producers demonstrated higher resistance to multiple antimicrobials, they exhibited lower intracellular survival rates. The results of this study illustrated the distribution, antimicrobial susceptibility profiles, genotype, and the ability of biofilm production and mammary epithelial cells invasion of these S. aureus isolates. This study can provide the basis for the development of a disease prevention program in dairy farms to reduce the potential risk in both animal and human health.
Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality Ministry of Agriculture, Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
Lili
L
Zhang
Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality Ministry of Agriculture, Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
Yuchen
Y
Li
Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality Ministry of Agriculture, Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
Hongduo
H
Bao
Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality Ministry of Agriculture, Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
Ruicheng
R
Wei
Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality Ministry of Agriculture, Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
Yan
Y
Zhou
Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality Ministry of Agriculture, Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
Hui
H
Zhang
Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality Ministry of Agriculture, Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China. Electronic address: Wangran2001@126.com.
Ran
R
Wang
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D018441:Biofilms / Q000254:growth & development; D002417:Cattle; D002681:China / Q000453:epidemiology; D005838:Genotype; D008414:Mastitis, Bovine / Q000453:epidemiology / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D008892:Milk / Q000382:microbiology*; D058885:Multilocus Sequence Typing; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000145:classification* / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*
D000900:Anti-Bacterial Agents
Biofilm production; Bovine mastitis; Invasion assay; MLST typing; Staphylococcus aureus