Is it the egg or the endometrium? Elevated progesterone on day of trigger is not associated with embryo ploidy nor decreased success rates in subsequent embryo transfer cycles.
OBJECTIVE
The purpose of our study was to determine if progesterone (P4) values on day of trigger affect certain cycle outcome parameters, ploidy status of embryos, as well as pregnancy outcomes in the subsequent first frozen embryo transfer cycle.


METHODS
Two hundred thirty-eight patients undergoing pre-gestational screening and freeze all protocol at our fertility center from 2013 to 2014 were included. Excluded patients were those whom had cancelled cycles prior to egg retrieval as well as cycles utilizing donor eggs. Once patients were identified as eligible for this study, frozen serum from the day of trigger was identified and analyzed using the Siemens Immulite 2000. Number of eggs retrieved, number of available embryos for biopsy, and number of euploid/aneuploid embryos were analyzed. The first frozen embryo transfer cycle was linked to the initial egg retrieval and outcomes including pregnancy rates, and live birth/ongoing pregnancy rates were calculated and analyzed. A discriminatory P4 value of 1.5 ng/ml was set. Group A had P4 values of less than 1.5 ng/ml and group B had P4 values greater than or equal to 1.5 ng/ml. T tests and chi-squared tests were used for statistical analysis.


RESULTS
Group A had an average trigger P4 value of 0.87 +/- 0.3 and group B had an average trigger P4 of 2.1 +/- 0.8. Table 1 shows the baseline characteristics of both group A and group B. The only significant difference between the two groups was total gonadotropin dosage (IU) with a p value of 0.02 and estradiol (pg/ml) at trigger, also with a p value of 0.02 (Table 1). Number of eggs retrieved, number of embryos biopsied, number euploid/aneuploid, and non-diagnosis embryos were all non-significant. Chi-square analysis was used to compare pregnancy rates between the two groups after the first frozen embryo transfer cycle. Group A had a pregnancy rate of 72 % and Group B had a pregnancy rate of 66.7 %, which was not significant. Ongoing pregnancy/live birth rates were 65.6 % in group A and 66.67 % in group B, also not significant (Table 2).


CONCLUSIONS
P4 values on day of trigger do not affect number of eggs retrieved and number of chromosomally normal embryos available for transfer in a subsequent embryo transfer cycle. Elevated P4 values (≥1.5 ng/ml) also do not affect pregnancy rates or live birth/ongoing pregnancy rates in the first subsequent frozen embryo transfer cycle.
New York University School of Medicine, 660 First Avenue, New York, NY, 10016, USA. Jason.kofinas@nyumc.org.
Jason D
JD
Kofinas
New York University School of Medicine, 660 First Avenue, New York, NY, 10016, USA.
Holly
H
Mehr
New York University School of Medicine, 660 First Avenue, New York, NY, 10016, USA.
Nandita
N
Ganguly
New York University School of Medicine, 660 First Avenue, New York, NY, 10016, USA.
Yelena
Y
Biley
New York University School of Medicine, 660 First Avenue, New York, NY, 10016, USA.
Svetlana
S
Bochkovsky
New York University School of Medicine, 660 First Avenue, New York, NY, 10016, USA.
David
D
McCulloh
New York University School of Medicine, 660 First Avenue, New York, NY, 10016, USA.
Jamie
J
Grifo
D000328:Adult; D001723:Birth Rate; D004624:Embryo Transfer; D004717:Endometrium / Q000378:metabolism / Q000473:pathology; D005260:Female; D005298:Fertility; D005307:Fertilization in Vitro / Q000379:methods; D006801:Humans; D010062:Ovulation Induction / Q000379:methods*; D010063:Ovum / Q000254:growth & development* / Q000473:pathology; D011003:Ploidies; D011247:Pregnancy; D018873:Pregnancy Rate; D011374:Progesterone / Q000097:blood*
D011374:Progesterone
Array comparative genomic hybridization; Frozen embryo transfer cycle; HCG trigger; Ploidy; Progesterone; Single embryo transfer