Identification and quantification of phenolic compounds in berry skin, pulp, and seeds in 13 grapevine varieties grown in Serbia.
Phenolic profile of 13 grapevine varieties was determined, with respect to three different parts of berries: skin, seed and pulp. Identification and quantification of the phenols was done using ultra-high performance liquid chromatography (UHPLC) coupled with a diode array detector and a triple-quadrupole mass spectrometer. The most abundant phenols in grape seeds were found to be flavan-3-ols, most of which are gallocatechin gallate and catechin. The skins were characterized mostly by flavonols, i.e. quercetin and myricetin. Characterization of anthocyanins in the berry skin by UHPLC coupled with Linear Trap Quadrupole and OrbiTrap mass analyzer revealed a total of twenty derivatives of malvidin, delphinidin, petunidin, cyanidin, and peonidin. To the extent of our knowledge this is the first work that shows the presence of malvidin 3,5-O-dihexoside in the berry skin of 'Merlot', 'Cabernet Franc', 'Shiraz' 'Sangiovese', 'Pinot Noir' and 'Prokupac', untypical for Vitis vinifera Linneo species.
Innovation Center, Faculty of Chemistry Ltd, University of Belgrade, 11158 Belgrade, Serbia.
Milica M
MM
Pantelić
Innovation Center, Faculty of Chemistry Ltd, University of Belgrade, 11158 Belgrade, Serbia.
Dragana Č
DČ
Dabić Zagorac
Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11080 Belgrade, Serbia.
Sonja M
SM
Davidović
Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11080 Belgrade, Serbia.
Slavica R
SR
Todić
Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11080 Belgrade, Serbia.
Zoran S
ZS
Bešlić
Faculty of Chemistry, University of Belgrade, P.O. Box 51, 11158 Belgrade, Serbia.
Uroš M
UM
Gašić
Faculty of Chemistry, University of Belgrade, P.O. Box 51, 11158 Belgrade, Serbia.
Živoslav Lj
ŽLj
Tešić
Faculty of Chemistry, University of Belgrade, P.O. Box 51, 11158 Belgrade, Serbia. Electronic address: mnatic@gmail.com.
Maja M
MM
Natić
D000872:Anthocyanins / Q000032:analysis; D002392:Catechin / Q000031:analogs & derivatives / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis; D044948:Flavonols / Q000032:analysis; D005504:Food Analysis / Q000379:methods*; D016166:Free Radical Scavengers; D005638:Fruit / Q000737:chemistry*; D013058:Mass Spectrometry; D010636:Phenols / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry; D059808:Polyphenols / Q000032:analysis; D012639:Seeds / Q000737:chemistry; D055771:Serbia; D027843:Vitis / Q000737:chemistry*
D000872:Anthocyanins; D005419:Flavonoids; D044948:Flavonols; D016166:Free Radical Scavengers; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; C417940:gallocatechin gallate; C065861:malvidin; C040015:myricetin; C017154:cyanidin; D002392:Catechin; C473205:peonidin; C017185:delphinidin; C473206:petunidin
Anthocyanins; Antioxidant activity; Grape; LC/MS; PCA; Phenolic profile