Purification of PCR Products to Improve STR Profiles.
Forensic laboratories routinely perform STR analyses using commercially available STR kits. Very low levels of DNA are extracted from many forensic samples. In these samples, the amount of DNA that can be placed in a PCR is below the optimal DNA range for the commercial kits, leading to weak profiles and allelic dropout. STR profiles generated from poor quality samples can be improved by purification of the PCR product prior to capillary electrophoresis.
Cellmark, Blacklands Way, Abingdon, Oxfordshire, OX14 1DY, UK. aroeder@cellmark.co.uk.
Amy D
AD
Roeder
D016172:DNA Fingerprinting / Q000379:methods* / Q000592:standards; D019075:Electrophoresis, Capillary; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*
Capillary electrophoresis; DNA; Forensic science; Post-PCR purification; STR profile