miR-486-5p attenuates tumor growth and lymphangiogenesis by targeting neuropilin-2 in colorectal carcinoma.
Increasing evidence suggests that microRNAs are associated with many important biologic processes in carcinogenesis. Despite ample research revealing the dysregualtion of miR-486-5p in various cancers, little is known about the roles of miR-486-5p in colorectal carcinoma (CRC). In this study, we investigated the biological functions and molecular mechanisms of miR-486-5p in CRC growth and invasion, discussing the potential of using miR-486-5p as a biomarker for colorectal cancers. Our data revealed that miR-486-5p was significantly downregulated in CRC tissues compared with the paracancer tissue by quantitative real-time polymerase chain reaction and that miR-486-5p was downregulated to a greater extent in advanced stage cancer (stage III and IV) as compared to early stage cancer (stage I and II). Luciferase reporter assay verified that neuropilin-2 was a direct functional target of miR-486-5p in the CRC cells, and upregulation of miR-486-5p in CRC cells negatively correlated with the expression of neuropilin-2. Furthermore, overexpression of miR-486-5p inhibited the tumor growth and lymphangiogenesis in nude mice, which was reversed by overexpression of neuropilin-2. Taken together, our study suggested miR-486-5p might be a suppressor of CRC.
Department of Gastroenterology, the Affiliated Hospital of Binzhou Medical University, Binzhou, Shandong Province, People's Republic of China.
Chengxia
C
Liu
Department of Gastroenterology, the Affiliated Hospital of Binzhou Medical University, Binzhou, Shandong Province, People's Republic of China.
Ming
M
Li
Department of Gastroenterology, the Affiliated Hospital of Binzhou Medical University, Binzhou, Shandong Province, People's Republic of China.
Yingbin
Y
Hu
Department of Gastroenterology, the Affiliated Hospital of Binzhou Medical University, Binzhou, Shandong Province, People's Republic of China.
Ning
N
Shi
Department of Gastroenterology, the Affiliated Hospital of Binzhou Medical University, Binzhou, Shandong Province, People's Republic of China.
Haisheng
H
Yu
Department of Gastroenterology, the Affiliated Hospital of Binzhou Medical University, Binzhou, Shandong Province, People's Republic of China.
Haiyan
H
Liu
Department of Gastroenterology, the Affiliated Hospital of Binzhou Medical University, Binzhou, Shandong Province, People's Republic of China.
Haifeng
H
Lian
colorectal carcinoma; lymphangiogenesis; miR-486-5p; neuropilin-2; tumor growth