NucleoSpin(®) XS Columns for DNA Concentration and Clean-Up.
The phenol-chloroform (organic) extraction method continues to be a preferred method for extraction of DNA from forensic evidence samples that may contain low quantities of DNA and polymerase chain reaction (PCR) inhibitors. The aqueous extracts from the organic extraction of DNA require subsequent concentration and cleanup, which has traditionally been performed with microdialysis filter units, including the Centricon(®) and Microcon(®) centrifugal filter devices. Here, we describe the use of the NucleoSpin(®) XS silica columns as an alternative for the concentration and purification of the aqueous extracts from the organic extraction and for the removal of PCR inhibitors from existing DNA extracts.
California Department of Justice, Jan Bashinski DNA Laboratory, Richmond, CA, 94804, USA. bill.hudlow@doj.ca.gov.|California Criminalistics Institute, 11181 Sun Center Drive, Rancho Cordova, CA, 95670, USA. bill.hudlow@doj.ca.gov.
William R
WR
Hudlow
D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D053803:Forensic Genetics / Q000379:methods; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D012822:Silicon Dioxide
D012822:Silicon Dioxide; D004247:DNA
DNA; DNA Extraction; Forensic; PCR Inhibitor