A pressure-based bioassay for the rapid, portable and quantitative detection of C-reactive protein.
A portable method for the rapid detection of the disease biomarker C-reactive protein (CRP) with a hand-held pressuremeter was developed. The method allows an ultrasensitive quantitation of CRP within the entire clinical range. The pressure-based method could facilitate CRP measurements in point-of-care testing (POCT) scenarios, such as clinical offices, emergency departments, and community service centers.
Cancer Center, Daping Hospital and Research Institution of Surgery, Third Military Medical University, Chongqing 400042, P. R. China. dongwang64@hotmail.com and The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Chenggong Hospital, Xiamen 361005, P. R. China. huilin@xmu.edu.cn.
Tianhai
T
Ji
The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Chenggong Hospital, Xiamen 361005, P. R. China. huilin@xmu.edu.cn.
Dan
D
Liu
The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Chenggong Hospital, Xiamen 361005, P. R. China. huilin@xmu.edu.cn.
Fang
F
Liu
The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Chenggong Hospital, Xiamen 361005, P. R. China. huilin@xmu.edu.cn.
Jiuxing
J
Li
The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Chenggong Hospital, Xiamen 361005, P. R. China. huilin@xmu.edu.cn.
Qingyu
Q
Ruan
The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Chenggong Hospital, Xiamen 361005, P. R. China. huilin@xmu.edu.cn.
Yanling
Y
Song
The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Chenggong Hospital, Xiamen 361005, P. R. China. huilin@xmu.edu.cn.
Tian
T
Tian
The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Chenggong Hospital, Xiamen 361005, P. R. China. huilin@xmu.edu.cn.
Zhi
Z
Zhu
The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Chenggong Hospital, Xiamen 361005, P. R. China. huilin@xmu.edu.cn.
Leiji
L
Zhou
The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Chenggong Hospital, Xiamen 361005, P. R. China. huilin@xmu.edu.cn.
Hui
H
Lin
The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, State Key Laboratory of Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Chenggong Hospital, Xiamen 361005, P. R. China. huilin@xmu.edu.cn.
Chaoyong
C
Yang
Cancer Center, Daping Hospital and Research Institution of Surgery, Third Military Medical University, Chongqing 400042, P. R. China. dongwang64@hotmail.com.
Dong
D
Wang
D001681:Biological Assay / Q000295:instrumentation*; D002097:C-Reactive Protein / Q000032:analysis*; D057230:Limit of Detection; D000067716:Point-of-Care Testing; D011312:Pressure; D013997:Time Factors
D002097:C-Reactive Protein