ent-Kaurenoic acid-rich extract from Mikania glomerata: In vitro activity against bacteria responsible for dental caries.
Many studies have reported that medicinal plant extracts can inhibit oral pathogen growth or adhesion to surfaces and therefore reduce dental caries formation. The addition of these extracts to oral products like mouthwashes and dentifrices is considered an important strategy in caries control. In this sense, we have developed a Mikania glomerata extract with high ent-kaurenoic acid content (KAMg). So, this work describes the preparation of such extract and the development of a validated HPLC-DAD method to determine its ent-kaurenoic acid (KA) content. Herein it is also described the KAMg in vitro antibacterial evaluation against several cariogenic bacteria in comparison with KA and the investigation of further aspects of the KAMg activity. Toxicological aspects of the developed extract were evaluated by assessing its cytotoxicity and genotoxicity. KA and a KA-rich extract like KAMg showed to inhibit the growth of microorganisms responsible for dental caries at relatively low MIC (Minimum inhibitory concentration) values, albeit not as low as the MIC value obtained for chlorhexidine digluconate (CHD), the golden anticariogenic standard approved by the American Dental Association Council on Dental Therapeutics. However, KAMg was more effective to inhibit the formation of a Streptococcus mutans biofilm with four times lower MICB50 (minimum inhibitory concentration that reduces 50% of the biofilm) value as compared with CHD. Taking into account all these data and considering the absence of genotoxic and cytotoxic activity under the tested conditions, it is suggested that KAMg is a natural product to be considered as active ingredient in oral care products.
University of Franca, Av. Dr. Armando Salles Oliveira, 201-Parque Universitário, Franca 14404-600, SP, Brazil.
Monique Rodrigues
MR
Moreira
University of Franca, Av. Dr. Armando Salles Oliveira, 201-Parque Universitário, Franca 14404-600, SP, Brazil.
Ariana Borges
AB
Souza
University of Franca, Av. Dr. Armando Salles Oliveira, 201-Parque Universitário, Franca 14404-600, SP, Brazil.
Sandra
S
Soares
University of Franca, Av. Dr. Armando Salles Oliveira, 201-Parque Universitário, Franca 14404-600, SP, Brazil.
Thamires Chiquini
TC
Bianchi
University of Franca, Av. Dr. Armando Salles Oliveira, 201-Parque Universitário, Franca 14404-600, SP, Brazil.
Daniele
D
de Souza Eugênio
University of Franca, Av. Dr. Armando Salles Oliveira, 201-Parque Universitário, Franca 14404-600, SP, Brazil.
Danieli Cristina
DC
Lemes
University of Franca, Av. Dr. Armando Salles Oliveira, 201-Parque Universitário, Franca 14404-600, SP, Brazil.
Carlos Henrique Gomes
CH
Martins
University of Franca, Av. Dr. Armando Salles Oliveira, 201-Parque Universitário, Franca 14404-600, SP, Brazil.
Thaís
T
da Silva Moraes
University of Franca, Av. Dr. Armando Salles Oliveira, 201-Parque Universitário, Franca 14404-600, SP, Brazil.
Denise Crispim
DC
Tavares
University of Franca, Av. Dr. Armando Salles Oliveira, 201-Parque Universitário, Franca 14404-600, SP, Brazil.
Natália Helen
NH
Ferreira
University of Franca, Av. Dr. Armando Salles Oliveira, 201-Parque Universitário, Franca 14404-600, SP, Brazil. Electronic address: Sergio.ambrosio@unifran.edu.br.
Sergio Ricardo
SR
Ambrósio
University of Franca, Av. Dr. Armando Salles Oliveira, 201-Parque Universitário, Franca 14404-600, SP, Brazil. Electronic address: rodrigo.veneziani@unifran.edu.br.
Rodrigo Cassio Sola
RC
Veneziani
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D003731:Dental Caries / Q000382:microbiology; D004224:Diterpenes / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008826:Microbial Sensitivity Tests; D035742:Mikania / Q000737:chemistry*; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology; D013295:Streptococcus mutans / Q000187:drug effects*; D018675:Toxicity Tests
D000900:Anti-Bacterial Agents; D004224:Diterpenes; D010936:Plant Extracts; C051436:kaurenoic acid
Biofilm inhibition; Dental caries; Mikania glomerata; Streptococcus mutans