Effects of Nutrients in Substrates of Different Grains on Aflatoxin B1 Production by Aspergillus flavus.
The current study was to better understand the potential factors affecting aflatoxin B1 (AFB1) accumulation varies between different grains. The nutrient composition and contents of defatted substrates were determined; additionally, according to the nutrient content of the substrates, the effects of starch, soluble sugars, amino acids, and trace elements on AFB1 production and mycelial growth in Czapek-Dox medium were examined. These results verified that removal of lipids from ground substrates significantly reduced the substrate's potential for AFB1 production by Aspergillus flavus. Maltose, glucose, sucrose, arginine, glutamic acid, aspartic acid, and zinc significantly induced AFB1 production up to 1.7- to 26.6-fold. And stachyose more significantly promoted A. flavus growth than the other nutrients. Thus, this study demonstrated that, combined with the nutrients content of grains, in addition to lipids, sucrose, stachyose, glutamic acid, and zinc might play key roles in various grains that are differentially infected by A. flavus. Particularly, two new nutrients (arginine and stachyose) of the grains we found significantly stimulate AFB1 production and A. flavus growth, respectively. The results provide new concepts for antifungal methods to protect food and animal feed from AFB1 contamination.
Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
Jie
J
Liu
Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
Lvhui
L
Sun
Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
Niya
N
Zhang
Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
Jiacai
J
Zhang
Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
Jiao
J
Guo
Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
Chong
C
Li
Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
Shahid Ali
SA
Rajput
Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
Desheng
D
Qi
D016604:Aflatoxin B1 / Q000096:biosynthesis*; D000596:Amino Acids / Q000032:analysis / Q000494:pharmacology; D001231:Aspergillus flavus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D002241:Carbohydrates / Q000032:analysis / Q000494:pharmacology; D003314:Corn Oil / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry*; D012995:Solubility; D013213:Starch / Q000032:analysis; D014131:Trace Elements / Q000032:analysis / Q000494:pharmacology
D000596:Amino Acids; D002241:Carbohydrates; D014131:Trace Elements; D003314:Corn Oil; D013213:Starch; D016604:Aflatoxin B1