Proteomics Unveils Fibroblast-Cardiomyocyte Lactate Shuttle and Hexokinase Paradox in Mouse Muscles.
Quantitative mapping, given in biochemically interpretable units such as mol per mg of total protein, of tissue-specific proteomes is prerequisite for the analysis of any process in cells. We applied label- and standard-free proteomics to characterize three types of striated muscles: white, red, and cardiac muscle. The analysis presented here uncovers several unexpected and novel features of striated muscles. In addition to differences in protein expression levels, the three muscle types substantially differ in their patterns of basic metabolic pathways and isoforms of regulatory proteins. Importantly, some of the conclusions drawn on the basis of our results, such as the potential existence of a "fibroblast-cardiomyocyte lactate shuttle" and the "hexokinase paradox" point to the necessity of reinterpretation of some basic aspects of striated muscle metabolism. The data presented here constitute a powerful database and a resource for future studies of muscle physiology and for the design of pharmaceutics for the treatment of muscular disorders.
Department of Animal Molecular Physiology, Wroclaw University , Wroclaw 50-205, Poland.
Dariusz
D
Rakus
Department of Animal Molecular Physiology, Wroclaw University , Wroclaw 50-205, Poland.
Agnieszka
A
Gizak
Biochemical Proteomics Group, Department of Proteomics and Signal Transduction, Max-Planck-Institute of Biochemistry , Martinsried 82152, Germany.
Jacek R
JR
Wiśniewski
D000818:Animals; D001692:Biological Transport; D005347:Fibroblasts / Q000378:metabolism*; D006593:Hexokinase; D019344:Lactic Acid / Q000378:metabolism*; D053858:Metabolic Networks and Pathways; D051379:Mice; D054792:Muscle, Striated / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*
D019344:Lactic Acid; D006593:Hexokinase
glycolysis; heart muscle; hexokinase; proteomic ruler; striated muscle; total protein approach