Taurine protects cisplatin induced cardiotoxicity by modulating inflammatory and endoplasmic reticulum stress responses.
Oxidative stress, ER stress, inflammation, and apoptosis results in the pathogenesis of cisplatin-induced cardiotoxicity. The present study was designed to investigate the signaling mechanisms involved in the ameliorating effect of taurine, a conditionally essential amino acid, against cisplatin-mediated cardiac ER stress dependent apoptotic death and inflammation. Mice were simultaneously treated with taurine (150 mg kg-1body wt, i.p.) and cisplatin (10 mg kg-1body wt, i.p.) for a week. Cisplatin exposure significantly altered serum creatine kinase and troponin T levels. In addition, histological studies revealed disintegration in the normal radiation pattern of cardiac muscle fibers. However, taurine administration could abate such adverse effects of cisplatin. Taurine administration significantly mitigated the reactive oxygen species production, alleviated the overexpression of nuclear factor-κB (NF-κB), and inhibited the elevation of proinflammatoy cytokines, adhesion molecules, and chemokines. Cisplatin exposure resulted in the unfolded protein response (UPR)-regulated CCAAT/enhancer binding protein (CHOP) up-regulation, induction of GRP78: a marker of ER stress and eIF2α signaling. Increase in calpain-1 expression level, activation of caspase-12 and caspase-3, cleavage of the PARP protein as well as the inhibition of antiapoptotic protein Bcl-2 were reflected on cisplatin-triggered apoptosis. Taurine could, however, combat against such cisplatin induced cardiac-abnormalities. The above mentioned findings suggest that taurine plays a beneficial role in providing protection against cisplatin-induced cardiac damage by modulating inflammatory responses and ER stress. © 2016 BioFactors, 42(6):647-664, 2016.
Division of Molecular Medicine, Bose Institute, P-1/12, CIT Scheme VII M, Kolkata, India.
Sayantani
S
Chowdhury
Division of Molecular Medicine, Bose Institute, P-1/12, CIT Scheme VII M, Kolkata, India.
Krishnendu
K
Sinha
Division of Molecular Medicine, Bose Institute, P-1/12, CIT Scheme VII M, Kolkata, India.
Sharmistha
S
Banerjee
Division of Molecular Medicine, Bose Institute, P-1/12, CIT Scheme VII M, Kolkata, India.
Parames C
PC
Sil
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000627:therapeutic use; D000970:Antineoplastic Agents / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects; D066126:Cardiotoxicity / Q000517:prevention & control*; D002374:Catalase / Q000378:metabolism; D002945:Cisplatin / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D000091342:Endoplasmic Reticulum Chaperone BiP; D059865:Endoplasmic Reticulum Stress; D006331:Heart Diseases / Q000139:chemically induced / Q000276:immunology / Q000517:prevention & control*; D015227:Lipid Peroxidation; D008297:Male; D051379:Mice; D009206:Myocardium / Q000201:enzymology / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013654:Taurine / Q000494:pharmacology* / Q000627:therapeutic use; D056811:Unfolded Protein Response
D000893:Anti-Inflammatory Agents; D000970:Antineoplastic Agents; D000091342:Endoplasmic Reticulum Chaperone BiP; C000717248:Hspa5 protein, mouse; D017382:Reactive Oxygen Species; D013654:Taurine; D002374:Catalase; D013482:Superoxide Dismutase; D002945:Cisplatin
cisplatin; heart; inflammation; oxidative and ER stress; taurine