Interactions at the Peptide/Silicon Surfaces: Evidence of Peptide Multilayer Assembly.
Selective deposition of peptides from liquid solutions to n- and p-doped silicon has been demonstrated. The selectivity is governed by peptide/silicon adhesion differences. A noninvasive, fast characterization of the obtained peptide layers is required to promote their application for interfacing silicon-based devices with biological material. In this study we show that spectroscopic ellipsometry-a method increasingly used for the investigation of biointerfaces-can provide essential information about the amount of adsorbed peptide material and the degree of coverage on silicon surfaces. We observed the formation of peptide multilayers for a strongly binding adhesion peptide on p-doped silicon. Application of the patterned layer ellipsometric evaluation method combined with Sellmeier dispersion led to physically consistent results, which describe well the optical properties of peptide layers in the visible spectral range. This evaluation allowed the estimation of surface coverage, which is an important indicator of adsorption quality. The ellipsometric findings were well supported by atomic force microscopy results.
ELI-ALPS, ELI-Hu Nkft , Dugonics ter 13, Szeged 6720, Hungary.
Zsuzsanna
Z
Pápa
Laboratoire Charles Coulomb (L2C), UMR 5221, CNRS-Université de Montpellier , Montpellier, France.|Nanobiology Institute, Yale University , West Haven, Connecticut 06516, United States.
Sathish Kumar
SK
Ramakrishnan
Laboratoire Charles Coulomb (L2C), UMR 5221, CNRS-Université de Montpellier , Montpellier, France.
Marta
M
Martin
Laboratoire Charles Coulomb (L2C), UMR 5221, CNRS-Université de Montpellier , Montpellier, France.
Thierry
T
Cloitre
Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences , Szeged, Hungary.
László
L
Zimányi
Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences , Szeged, Hungary.|Faculty of Chemical Sciences, Universidad Autónoma de San Luis Potosí , San Luis Potosí, Mexico.
Jessica
J
Márquez
ELI-ALPS, ELI-Hu Nkft , Dugonics ter 13, Szeged 6720, Hungary.
Judit
J
Budai
Zsolt
Z
Tóth
Laboratoire Charles Coulomb (L2C), UMR 5221, CNRS-Université de Montpellier , Montpellier, France.
Csilla
C
Gergely
D010455:Peptides / Q000737:chemistry*; D012825:Silicon / Q000737:chemistry*; D013499:Surface Properties
D010455:Peptides; D012825:Silicon