Prognostic value of decreased expression of RBM4 in human gastric cancer.
RNA-binding motif 4 (RBM4) is a multifunctional protein that participates in regulating alternative splicing and mRNA translation. Its reduced expression has been associated with poor overall survival in lung cancer, breast cancer and ovarian cancer. We assessed RBM4 protein expression levels with immunohistochemistry in tissue microarrays containing malignant gastric cancer tissues and benign tissues from 813 patients. We also examined the expression levels of RBM4 mRNA in twenty-five paired gastric cancer samples and adjacent noncancerous tissues. Both RBM4 protein and mRNA expression levels were significantly lower in gastric cancer tissues compared with the adjacent noncancerous tissues. There was a significant association between reduced RBM4 protein expression and differentiation (P < 0.001), lymph node metastasis (P = 0.026), TNM state (P = 0.014) and distant metastasis (P = 0.036). Patients with reduced RBM4 expression (P < 0.001, CI = 0.315-0.710) and TNM stage III and IV (P < 0.001, CI = 4.757-11.166) had a poor overall survival. These findings suggest that RBM4 is a new biomarker in gastric cancer, as the reduced expression of this protein is correlated with poor differentiation, lymph node status and distant metastasis. Further, lower RBM4 expression is an independent prognostic marker for gastric cancer.
Department of Pathology, Nanjing Medical University, Nanjing 210029, China.|Department of Oncology, Huai'an Hospital Affiliated of Xuzhou Medical College and Huai'an Second People's Hospital, Huai'an 223002, China.
Hongmei
H
Yong
Department of Pathology, Affiliated Hospital of Nantong University, Nantong 226019, China.
Huijun
H
Zhu
Department of Pathology, Affiliated Hospital of Nantong University, Nantong 226019, China.
Shu
S
Zhang
School of Public Health, Nantong University, Nantong 226000, China.
Wei
W
Zhao
Department of Pathology, Affiliated Hospital of Nantong University, Nantong 226019, China.
Wei
W
Wang
Department of Pathology, Nanjing Medical University, Nanjing 210029, China.|Department of Oncology, Second Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China.
Chen
C
Chen
Department of Pathology, Nanjing Medical University, Nanjing 210029, China.
Guipeng
G
Ding
Department of Oncology, Huai'an Hospital Affiliated of Xuzhou Medical College and Huai'an Second People's Hospital, Huai'an 223002, China.
Lun
L
Zhu
Department of Oncology, Huai'an Hospital Affiliated of Xuzhou Medical College and Huai'an Second People's Hospital, Huai'an 223002, China.
Ziyuan
Z
Zhu
Department of Oncology, Huai'an Hospital Affiliated of Xuzhou Medical College and Huai'an Second People's Hospital, Huai'an 223002, China.
Huaidong
H
Liu
Department of Oncology, Huai'an Hospital Affiliated of Xuzhou Medical College and Huai'an Second People's Hospital, Huai'an 223002, China.
Yongjie
Y
Zhang
Department of Epidemiology, School of Public Health, Nanjing Medical University, Nanjing 210029, China.
Jinbo
J
Wen
Department of General Surgery, Drum Tower Hospital, Medical School of Nanjing University, Nanjing 210008, China.
Xing
X
Kang
Key Laboratory of Antibody Technique of Ministry of Health, Nanjing Medical University, Nanjing 210029, China.|Huadong Medical Institute of Biotechniques, Nanjing 210029, China.
Jin
J
Zhu
Department of Pathology, Nanjing Medical University, Nanjing 210029, China.|Key Laboratory of Antibody Technique of Ministry of Health, Nanjing Medical University, Nanjing 210029, China.|Jiangsu Collaborative Innovation Center For Cancer Personalized Medicine, Nanjing Medical University, Nanjing 210029, China.
Zhenqing
Z
Feng
The Comprehensive Cancer Center, Drum Tower Hospital, Medical School of Nanjing University &Clinical Cancer Institute of Nanjing University, Nanjing 210008 China.
Baorui
B
Liu
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D011379:Prognosis; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000401:mortality; D016019:Survival Analysis; D046888:Tissue Array Analysis
D014408:Biomarkers, Tumor; C473692:RBM4 protein, human; D016601:RNA-Binding Proteins