Evaluation of four commonly used normalizer genes for the study of decidual gene expression.
Reverse transcription quantitative PCR (RT-qPCR) gene expression results must be normalized using stably expressed genes to correct for technical variation. We evaluated the expression of four widely used normalizers (RNA18S, GAPDH, TBP, and YWHAZ) across 59 decidual tissue samples collected by vacuum suction from preeclamptic and normotensive pregnancies. RNA18S and GAPDH were not suitable as normalizers, while YWHAZ and TBP were stably expressed across the study groups.
Department of Obstetrics and Department of Gynecology, Oslo University Hospital, Ullevål, Norway; Faculty of Medicine, University of Oslo, Norway; Institute for Experimental Medical Research, Oslo University Hospital and University of Oslo, Oslo, Norway.
Ana Rita Sequeira de
AR
Sousa
Department of Obstetrics and Department of Gynecology, Oslo University Hospital, Ullevål, Norway; Faculty of Medicine, University of Oslo, Norway.
Anne Cathrine
AC
Staff
Department of Obstetrics and Department of Gynecology, Oslo University Hospital, Ullevål, Norway; Faculty of Medicine, University of Oslo, Norway; Institute for Experimental Medical Research, Oslo University Hospital and University of Oslo, Oslo, Norway.
Guro Mørk
GM
Johnsen
Department of Obstetrics and Department of Gynecology, Oslo University Hospital, Ullevål, Norway; Faculty of Medicine, University of Oslo, Norway.
Mina Susanne
MS
Weedon-Fekjær
Department of Obstetrics and Department of Gynecology, Oslo University Hospital, Ullevål, Norway; Faculty of Medicine, University of Oslo, Norway; Institute for Experimental Medical Research, Oslo University Hospital and University of Oslo, Oslo, Norway. Electronic address: g.l.storvold@medisin.uio.no.
Gro Leite
GL
Størvold
D048948:14-3-3 Proteins / Q000235:genetics / Q000378:metabolism; D003656:Decidua / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000379:methods; D020043:Genes, Essential / Q000235:genetics*; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D012337:RNA, Ribosomal, 18S / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D035181:TATA-Box Binding Protein / Q000235:genetics / Q000378:metabolism
D048948:14-3-3 Proteins; D012333:RNA, Messenger; D012337:RNA, Ribosomal, 18S; D035181:TATA-Box Binding Protein; C509817:TBP protein, human; C572235:YWHAZ protein, human
Decidua basalis; Gene expression; Housekeeping gene; Normalizer gene; Preeclampsia; Quantitative PCR