In vivo assessment of coronary flow and cardiac function after bolus adenosine injection in adenosine receptor knockout mice.
Bolus injections of adenosine and the A2A adenosine receptor (AR) selective agonist (regadenoson) are used clinically as a substitute for a stress test in people who cannot exercise. Using isolated tissue preparations, our lab has shown that coronary flow and cardiac effects of adenosine are mostly regulated by the AR subtypes A1, A2A, and A2B In this study, we used ultrasound imaging to measure the in vivo effects of adenosine on coronary blood flow (left coronary artery) and cardiac function in anesthetized wild-type, A1 knockout (KO), A2AKO, A2BKO, A3KO, A1, and A3 double KO (A1/3 DKO) and A2A and A2B double KO (A2A/2B DKO) mice in real time. Echocardiographic and Doppler studies were performed using a Visualsonic Vevo 2100 ultrasound system. Coronary blood flow (CBF) baseline data were obtained when animals were anesthetized with 1% isoflourane. Diameter (D) and velocity time integral (VTI) were measured on the left coronary arteries (CBF = ((π/4) × D(2) × VTI × HR)/1000). CBF changes were the highest within 2 min of injection (about 10 mg/kg). Heart rate, cardiac output, and stroke volume were measured by tracing the left ventricle long axis. Our data support a role for the A2 AR in CBF and further support our conclusions of previous studies from isolated tissues. Adenosine-mediated decreases in cardiac output and stroke volume may be A2B and/or A3 AR-mediated; however, the A1 and A2 ARs also play roles in overall cardiac function. These data further provide a powerful translational tool in studying the cardiovascular effects of adenosine in disease states.
Department of Physiology & Pharmacology, West Virginia University, Morgantown, West Virginia bteng@hsc.wvu.edu.
Bunyen
B
Teng
Department of Medicine, University of North Carolina, Chapel Hill, North Carolina.
Stephen L
SL
Tilley
Universite Libre de Bruxelles, Brussels, Belgium.
Catherine
C
Ledent
Department of Physiology & Pharmacology, West Virginia University, Morgantown, West Virginia.
S Jamal
SJ
Mustafa
D000241:Adenosine / Q000494:pharmacology*; D058908:Adenosine A2 Receptor Agonists / Q000494:pharmacology*; D000818:Animals; D001783:Blood Flow Velocity / Q000187:drug effects*; D002302:Cardiac Output / Q000187:drug effects; D003331:Coronary Vessels / Q000187:drug effects*; D051379:Mice; D018345:Mice, Knockout; D018047:Receptors, Purinergic P1 / Q000235:genetics* / Q000378:metabolism; D012039:Regional Blood Flow / Q000187:drug effects*; D013318:Stroke Volume / Q000187:drug effects
D058908:Adenosine A2 Receptor Agonists; D018047:Receptors, Purinergic P1; D000241:Adenosine
Adenosine receptor knockout mice; adenosine receptors; cardiac output; coronary flow; ejection fraction; heart rate; stroke volume; ultrasound