The Presence of Endometrial Cells in Peritoneal Fluid of Women With and Without Endometriosis.
To reinforce Sampson's theory of retrograde menstruation in the pathogenesis of endometriosis, proof should be provided that during menstruation endometrial cells are present in peritoneal fluid (PF). We hypothesize that the prevalence of PF samples containing endometrial cells is higher in patients with endometriosis than in controls without endometriosis during menstruation. We selected from our biobank PF samples of 17 reproductive-age women with (n = 9) or without (n = 8) endometriosis who had received a diagnostic laparoscopy for investigation of pain/infertility. Peritoneal fluid had been collected during laparoscopy in the menstrual phase of the cycle, centrifuged, and the resulting pellet was stored at -80°C. About 5-μm sections of frozen PF pellets were stained using the Dako Envision Flex system with primary antibodies against epithelial cell adhesion molecule (Ep-CAM; endometrial epithelial cells), CD10 (endometrial stromal cells), prekeratin (epithelial/mesothelial cells), vimentin (endometrial/mesothelial/immune cells), calretinin (mesothelial cells), and CD68 (macrophages). The PF cells positive for Ep-CAM were detected in 5 of 9 patients with endometriosis and 6 of 8 controls ( P = .62). CD10 stained positively in 6 of the 9 patients with endometriosis and 3 of the 8 controls ( P = .35). Calretinin and prekeratin staining showed the presence of mesothelial cells in all pellets. Vimentin stained approximately 100% of the PF cells. CD68+ macrophages represented >50% of cells in all pellets. The prevalence of PF samples containing endometrial epithelial and stromal cells was not higher in patients with endometriosis than in controls without endometriosis during menstruation. Our findings question the relevance of endometrial cells in PF for the pathogenesis of endometriosis and support the importance of other mechanisms such as immune dysfunction and/or endometrial stem cells.
1 Department of Development and Regeneration, Organ systems, KU Leuven, Leuven, Belgium.|2 Department of Obstetrics and Gynaecology, Leuven University Fertility Centre, University Hospital Leuven, Leuven, Belgium.
Dorien F
DF
O
3 Department of Imaging and Pathology, KU Leuven and University Hospitals Leuven, Leuven, Belgium.
Tania
T
Roskams
3 Department of Imaging and Pathology, KU Leuven and University Hospitals Leuven, Leuven, Belgium.
Kathleen
K
Van den Eynde
1 Department of Development and Regeneration, Organ systems, KU Leuven, Leuven, Belgium.|2 Department of Obstetrics and Gynaecology, Leuven University Fertility Centre, University Hospital Leuven, Leuven, Belgium.
Arne
A
Vanhie
1 Department of Development and Regeneration, Organ systems, KU Leuven, Leuven, Belgium.|2 Department of Obstetrics and Gynaecology, Leuven University Fertility Centre, University Hospital Leuven, Leuven, Belgium.
Daniëlle P
DP
Peterse
2 Department of Obstetrics and Gynaecology, Leuven University Fertility Centre, University Hospital Leuven, Leuven, Belgium.
Christel
C
Meuleman
2 Department of Obstetrics and Gynaecology, Leuven University Fertility Centre, University Hospital Leuven, Leuven, Belgium.
Carla
C
Tomassetti
2 Department of Obstetrics and Gynaecology, Leuven University Fertility Centre, University Hospital Leuven, Leuven, Belgium.
Karen
K
Peeraer
1 Department of Development and Regeneration, Organ systems, KU Leuven, Leuven, Belgium.
Thomas M
TM
D'Hooghe
1 Department of Development and Regeneration, Organ systems, KU Leuven, Leuven, Belgium.|2 Department of Obstetrics and Gynaecology, Leuven University Fertility Centre, University Hospital Leuven, Leuven, Belgium.
Amelie
A
Fassbender
D000328:Adult; D001202:Ascitic Fluid / Q000378:metabolism / Q000473:pathology*; D015415:Biomarkers / Q000378:metabolism; D004715:Endometriosis / Q000378:metabolism / Q000473:pathology*; D004717:Endometrium / Q000378:metabolism / Q000473:pathology*; D000071858:Epithelial Cell Adhesion Molecule / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000378:metabolism / Q000473:pathology*; D008264:Macrophages / Q000378:metabolism / Q000473:pathology; D008598:Menstruation / Q000378:metabolism; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology; D014746:Vimentin / Q000378:metabolism
D015415:Biomarkers; C499379:EPCAM protein, human; D000071858:Epithelial Cell Adhesion Molecule; D014746:Vimentin
endometrial cells; endometriosis; immunocytochemistry; menstruation; peritoneal fluid