Characterization, specificity and sensibility of produced anti-Rhamdia quelen vitellogenin in Brazilian fish species.
Endocrine-disrupting chemicals (EDCs) are widespread used and can interfere on hormone regulation with adverse consequences for both biota and human. Vitellogenin (vtg) is a yolk precursor protein synthesized by the liver in response to estrogen. In order to characterize the vtg of tropical fish Rhamdia quelen and establish a molecular biomarker, adult male individuals were exposed to 17-β-estradiol (E2) for vtg induction and anti-R. quelen vtg polyclonal antibodies production. Vitellogenic female fish were used as positive control group. E2-induced vtg was characterized as a glycolipophosphoprotein of high molecular mass with peptide mass fingerprint very similar in E2-exposed male and vitellogenic female fish. A polyclonal serum containing anti-R. quelen vtg antibodies was produced and showed high specificity and sensibility to detect the vtg of three fish species: R. quelen, Piaractus mesopotamicus and Prochilodus lineatus. Wildlife and laboratory studies reported that EDCs released into the environment may alter the levels of plasma vtg in male fish, making this protein a valuable biomarker of xenoestrogens exposure. Then, we propose the use of anti-R. quelen vtg as a tool for biomonitoring studies and water quality assessment in Brazil and South American countries where the three fish species occur.
Laboratório de Toxicologia Celular, Departamento de Biologia Celular, Universidade Federal do Paraná, Rua Francisco H. dos Santos, PO Box 19031, Curitiba, PR, Brazil. danidmc87@gmail.com.
Daniele Dietrich
http://orcid.org/0000-0002-0206-0934
DD
Moura Costa
Laboratório de Toxicologia Celular, Departamento de Biologia Celular, Universidade Federal do Paraná, Rua Francisco H. dos Santos, PO Box 19031, Curitiba, PR, Brazil.
Dandie Antunes
DA
Bozza
Departamento de Patologia Básica, Universidade Federal do Paraná, PO Box 19031, Curitiba, PR, Brazil.
Luiz Eduardo
LE
Rizzo
Estação de Piscicultura Panamá, Paulo Lopes, SC, Brazil.
Juan
J
Garcia
Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Ponta Grossa, PR, Brazil.
Michele Dietrich Moura
MD
Costa
Laboratório de Toxicologia Celular, Departamento de Biologia Celular, Universidade Federal do Paraná, Rua Francisco H. dos Santos, PO Box 19031, Curitiba, PR, Brazil.
Ciro Alberto
CA
de Oliveira Ribeiro
D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000378:metabolism; D001938:Brazil; D002397:Catfishes / Q000097:blood* / Q000378:metabolism; D060125:Characiformes / Q000097:blood* / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology; D005260:Female; D029941:Fish Proteins / Q000097:blood* / Q000276:immunology / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D009865:Oocytes / Q000378:metabolism; D013737:Testis / Q000378:metabolism; D014819:Vitellogenins / Q000097:blood* / Q000276:immunology / Q000378:metabolism
D000906:Antibodies; D004967:Estrogens; D029941:Fish Proteins; D014819:Vitellogenins; D004958:Estradiol
Freshwater fish; Molecular biomarkers; Tropical fish; Vitellogenin; Xenoestrogen