Expect the Unexpected Enrichment of "Hidden Proteome" of Seeds and Tubers by Depletion of Storage Proteins.
Dynamic resolution of seed and tuber protein samples is highly limited due to the presence of high-abundance storage proteins (SPs). These proteins inevitably obscure the low-abundance proteins (LAPs) impeding their identification and characterization. To facilitate the detection of LAPs, several methods have been developed during the past decade, enriching the proteome with extreme proteins. Most of these methods, if not all, are based on the specific removal of SPs which ultimately magnify the proteome coverage. In this mini-review, we summarize the available methods that have been developed over the years for the enrichment of LAPs either from seeds or tubers. Incorporation of these methods during the protein extraction step will be helpful in understanding the seed/tuber biology in greater detail.
Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University Miryang, South Korea.
Ravi
R
Gupta
Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University Miryang, South Korea.
Cheol W
CW
Min
Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research Cologne, Germany.
Yiming
Y
Wang
Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University Miryang, South Korea.
Yong C
YC
Kim
Research Laboratory for Biotechnology and BiochemistryKathmandu, Nepal; Global Research Arch for Developing Education, Academy Pvt. Ltd.Birgunj, Nepal.
Ganesh K
GK
Agrawal
Global Research Arch for Developing Education, Academy Pvt. Ltd.Birgunj, Nepal; Faculty of Health and Sport Sciences and Tsukuba International Academy for Sport Studies, University of TsukubaIbaraki, Japan.
Randeep
R
Rakwal
Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University Miryang, South Korea.
Sun T
ST
Kim
depletion methods; hidden proteome; high-abundance proteins; low-abundance proteins; plant; proteome coverage; storage proteins