Cell-Free Technologies for Proteomics and Protein Engineering.
Cell-free translation systems facilitate rapid production of specific proteins and are particularly suited as high-throughput methods for whole-genome protein synthesis. Moreover, these systems do not rely on living cells, thereby allowing the synthesis of unstable or cytotoxic proteins in vitro. In this review, we describe the principles and potential applications of cell-free protein translation systems and the future prospects of proteomics approaches using next-generation sequencing and cell-free expression technologies.
Hiroyuki
H
Ohashi
Division of Molecular Biology, Tokyo University of Science Research Institute for Biomedical Science, 2669 Yamazaki, Noda, Chiba 278-0022, Japan. nekoneko@rs.tus.ac.jp.
Etsuko
E
Miyamoto-Sato
D002474:Cell-Free System / Q000378:metabolism*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D015202:Protein Engineering / Q000379:methods; D011506:Proteins / Q000378:metabolism*; D040901:Proteomics / Q000379:methods
D011506:Proteins