Histopathologic Characterization and Shedding Dynamics of Guineafowl (Numida meleagris) Intravenously Infected with a H6N2 Low Pathogenicity Avian Influenza Virus.
Guineafowl of different ages were inoculated intravenously with a H6N2 wild waterfowl-origin low pathogenicity avian influenza virus (LPAIV). No clinical disease was observed. The infected birds had atrophy of the spleen, thymus, and cloacal bursa when compared with the noninfected control groups. The central and peripheral lymphoid tissues presented either lymphoproliferative or degenerative lesions that increased in intensity from 14 to 21 days postinoculation (DPI). Lymphoid depletion was present in the bursa, thymic lobes, and spleen T-dependent zone. In contrast, lymphoid proliferation was observed in liver, pancreas, and spleen B-dependent zone. Bronchus associated lymphoid tissue hyperplasia was observed in the lungs of the birds at 14 and 21 DPI. The virus was detected by virus isolation and reverse transcription PCR from both oropharyngeal and cloacal swabs with higher isolation rates from the latter. Most birds from the LPAIV inoculated groups shed virus up to 7 DPI. The virus was infrequently isolated from lung, kidney, liver, bursa, or spleen of infected birds until 14 DPI and from two samples (kidney and spleen, 1-yr-old birds) at 21 DPI. These data indicate that the wild bird-origin LPAIV used in this study caused pantropic infection in guineafowl when inoculated intravenously.
A National Diagnostic and Research Veterinary Medicine Institute, 15 P. Slaveikov Boulevard, Sofia, 1606, Bulgaria.|B Southeast Poultry Research Laboratory, Agricultural Research Service, United States Department of Agriculture (USDA), 934 College Station Road, Athens, GA 30605.
Kiril M
KM
Dimitrov
C Department of Microbiology, Infection and Parasite Diseases, Faculty of Veterinary Medicine, Trakia University, Students Campus, 6000 Stara Zagora, Bulgaria.
Ivan S
IS
Zarkov
D Department of General and Clinical Pathology, Faculty of Veterinary Medicine, Trakia University, Students Campus, 6000 Stara Zagora, Bulgaria.
Ivan
I
Dinev
A National Diagnostic and Research Veterinary Medicine Institute, 15 P. Slaveikov Boulevard, Sofia, 1606, Bulgaria.
Gabriela V
GV
Goujgoulova
B Southeast Poultry Research Laboratory, Agricultural Research Service, United States Department of Agriculture (USDA), 934 College Station Road, Athens, GA 30605.
Patti J
PJ
Miller
B Southeast Poultry Research Laboratory, Agricultural Research Service, United States Department of Agriculture (USDA), 934 College Station Road, Athens, GA 30605.
David L
DL
Suarez
D000818:Animals; D000835:Animals, Wild / Q000821:virology; D046094:Galliformes / Q000821:virology*; D009980:Influenza A virus / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology*; D005585:Influenza in Birds / Q000473:pathology / Q000821:virology*; D008099:Liver / Q000473:pathology / Q000821:virology; D013154:Spleen / Q000473:pathology / Q000821:virology; D014774:Virulence; D017201:Virus Shedding
H6N2; LPAI; avian influenza; guineafowl; histopathology; rRT-PCR; shedding