Barrier function of the skin in a repetitive irritation model and influence of 2 different treatments.
OBJECTIVE
The aim of this study was to develop and characterize an experimental model of cumulative irritant contact dermatitis using the repetitive application over 2 weeks of 2 different irritative stimuli, sodium lauryl sulphate (SLS) in low concentration and toluene. Further, it was intended to look at the effect of 2 different treatments which could influence the regeneration of the skin barrier, as already investigated in preliminary short-term experiments.


METHODS
Occlusive short-time daily application of the irritants (SLS 30 min; toluene 10 min) was performed. After removal of the occlusive patch, the irritated areas were treated with 2 different preparations, Lotio Alba Aquosa and vaseline (white petroleum jelly). Bioengineering techniques were used to precisely measure the skin condition.


RESULTS
A mild irritation was obtained under these experimental conditions. The bioengineering measurements allowed us to differentiate both irritants through their different influences on trans-epidermal water loss and skin hydration. Treatment with vaseline did not significantly influence the course of the irritation, but Lotio Alba Aquosa clearly potentiated it, whereby different effects were noticed for both irritative stimuli.


CONCLUSIONS
The use of bioengineering techniques in this model of mild cumulative irritation allowed us to differentiate the action of both irritants, showing that they act through different mechanisms. The results of the treatments pointed out the need to treat an irritation with the right product for the right time period.
Biopharmacy Department, Spirig Ltd., Pharmaceuticals, EgerkingenDepartment of Dermatology, University Hospital, Zürich, Switzerland.
B
B
Gabard
Biopharmacy Department, Spirig Ltd., Pharmaceuticals, EgerkingenDepartment of Dermatology, University Hospital, Zürich, Switzerland.
P
P
Eisner
Biopharmacy Department, Spirig Ltd., Pharmaceuticals, EgerkingenDepartment of Dermatology, University Hospital, Zürich, Switzerland.
P
P
Treffel
bioengineering measurements; irritant contact dermatitis; sodium lauryl sulphate; toluene