A Modified Carbon Monoxide Breath Test for Measuring Erythrocyte Lifespan in Small Animals.
This study was to develop a CO breath test for RBC lifespan estimation of small animals. The ribavirin induced hemolysis rabbit models were placed individually in a closed rebreath cage and air samples were collected for measurement of CO concentration. RBC lifespan was calculated from accumulated CO, blood volume, and hemoglobin concentration data. RBC lifespan was determined in the same animals with the standard biotin-labeling method. RBC lifespan data obtained by the CO breath test method for control (CON, 49.0 ± 5.9 d) rabbits, rabbits given 10 mg/kg·d(-1) of ribavirin (RIB10, 31.0 ± 4.0 d), and rabbits given 20 mg/kg·d(-1) of ribavirin (RIB20, 25.0 ± 2.9 d) were statistically similar (all p > 0.05) to and linearly correlated (r = 0.96, p < 0.01) with the RBC lifespan data obtained for the same rabbits by the standard biotin-labeling method (CON, 51.0 ± 2.7 d; RIB10, 33.0 ± 1.3 d; and RIB20, 27.0 ± 0.8 d). The CO breath test method takes less than 3 h to complete, whereas the standard method requires at least several weeks. In conclusion, the CO breath test method provides a simple and rapid means of estimating RBC lifespan and is feasible for use with small animal models.
Institute of Breath Test Research, Shenzhen University, Shenzhen 518060, China.
Yong-Jian
YJ
Ma
Institute of Breath Test Research, Shenzhen University, Shenzhen 518060, China; Department of Gastroenterology, Nanshan Hospital, Guangdong Medical College, Shenzhen 518052, China.
Hou-De
HD
Zhang
Institute of Seekya Breath Test Technology, Shenzhen 518053, China.
Yong-Qiang
YQ
Ji
Institute of Seekya Breath Test Technology, Shenzhen 518053, China.
Guo-Liang
GL
Zhu
Institute of Seekya Breath Test Technology, Shenzhen 518053, China.
Jia-Liang
JL
Huang
Institute of Seekya Breath Test Technology, Shenzhen 518053, China.
Li-Tao
LT
Du
Shenzhen Testing Center of Medical Devices, Shenzhen 518000, China.
Ping
P
Cao
Shenzhen Testing Center of Medical Devices, Shenzhen 518000, China.
De-Yue
DY
Zang
Central Laboratory, Nanshan Hospital, Guangdong Medical College, Shenzhen 518052, China.
Ji-Hui
JH
Du
Central Laboratory, Nanshan Hospital, Guangdong Medical College, Shenzhen 518052, China.
Rong
R
Li
Central Laboratory, Nanshan Hospital, Guangdong Medical College, Shenzhen 518052, China.
Lei
L
Wang
D000818:Animals; D001710:Biotin / Q000378:metabolism; D001944:Breath Tests / Q000379:methods*; D002248:Carbon Monoxide / Q000032:analysis*; D016922:Cellular Senescence / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D008297:Male; D011817:Rabbits; D012254:Ribavirin / Q000494:pharmacology; D013194:Staining and Labeling
D012254:Ribavirin; D001710:Biotin; D002248:Carbon Monoxide