Discrepancies between Antigen and Polymerase Chain Reaction Tests for the Detection of Rotavirus and Norovirus.
We compared the results of an antigen test (ELISA) with those of polymerase chain reaction (PCR) for the detection of rotavirus and norovirus in stool specimens. Rotavirus and norovirus antigen-positive stool specimens were collected, and rotavirus and norovirus PCRs were performed on these specimens. Of the 325 rotavirus antigen-positive specimens, 200 were positive for both assays and 125 were PCR negative. Of 286 norovirus antigen-positive specimens, 51 were PCR negative. Comparison of the lower limit of detection showed that rotavirus PCR was 16 times more sensitive and norovirus PCR was over 4,000 times more sensitive than the ELISA. Discrepant results between ELISA and PCR were common, and the possibility of false-positive and false-negative results should be considered with rotavirus and norovirus assays.
Department of Laboratory Medicine, Hallym University College of Medicine, Hwaseong, Korea hskim0901@empas.com.
Hyun Soo
HS
Kim
Department of Laboratory Medicine, Hallym University College of Medicine, Hwaseong, Korea.
Jae-Seok
JS
Kim
D000956:Antigens, Viral / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D029322:Norovirus / Q000276:immunology* / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012401:Rotavirus / Q000276:immunology* / Q000302:isolation & purification*
D000956:Antigens, Viral
ELISA; PCR; comparison; norovirus; rotavirus