[Effect of Qidan Granule on PMC Derived Peptide Content and Structure of Hippocampal CA1 Region in Microwave Radiated Rats].
OBJECTIVE
To explore the protection of high intensity microwave radiation on hypothalamo-pituitary-adrenal axis (HPAA) activity and hippocampal CA1 structure in rats and the protectiveeffect of Qindan Granule (QG) on radiation injured rats.


METHODS
Totally 48 Wistar rats were randomlydivided into 8 groups, i.e., the normal control group, post-radiation day 1, 7, and 10 groups, 7 and 10days prevention groups, day 7 and 10 treatment groups, 6 in each group. Rats in prevention groups wererespectively administered with QG liquid (1 mL/100 g, 4. 75 g crude drugs) for 7 days and 10 days bygastrogavage and then microwave radiation. Then preventive effect for radiation injury was statisticallycalculated with the normal control group and the post-radiation day 1 group. Rats in treatment groupswere firstly irradiated, and then administered with QG liquid (1 mL/100 g, 4.75 g crude drugs). Finally preventive effect for radiation injury was statistically calculated with the normal control group, post-radiation day 7 and 10 groups. Contents of corticotrophin releasing hormone (CRH), beta endorphin (beta-EP), adrenocorticotropic hormone (ACTH), and heat shock protein 70 (HSP70) were detected. Morphological changes and structure of hippocampal CA1 region were observed under light microscope.


RESULTS
Compared with the normal control group, contents of CRH and beta-EP significantly decreased in each radiation group. Serum contents of ACTH and beta-EP significantly increased in post-radiation day 1 and 7 groups (P < 0.05). Compared with radiation groups, beta-EP content in serum and pituitary significantly increased, and serum ACTH content significantly decreased in prevention groups (P < 0.05). Pituitary contents of CRH and beta-EP significantly increased in prevention groups. Serum contents of ACTH, beta-EP, and HSP70 were significantly lower in day 7 treatment group than post-radiation day 7 group (P < 0.05). Morphological results showed that pyramidal neurons in the hippocampal CA1 region arranged in disorder, with swollen cells, shrunken and condensed nucleus, dark dyeing cytoplasm, unclear structure. Vessels in partial regions were dilated with static blood; tissues were swollen and sparse. In prevention and treatment groups pathological damage of hippocampal CA1 region was obviously attenuated; neurons were arranged more regularly; swollen, pycnotic, or deleted neuron number were decreased; vascular dilatation and congestion was lessened.


CONCLUSIONS
QG could affect HPAA function and activity of high intensity microwave radiated rats, showing certain preventive and therapeutic effects of microwave radiated rats by adjusting synthesis and release of partial bioactive peptides and hormones in HPAA, improving pathological injury in hippocampal CA1 region.
Lan-fang
LF
Guan
Ya-wei
YW
Li
Jun-jie
JJ
Xu
Xiu-hong
XH
Zhong
Hong
H
Zhang
Wen-he
WH
Zhu
Shi-jie
SJ
Lv
D000324:Adrenocorticotropic Hormone / Q000097:blood; D000818:Animals; D056547:CA1 Region, Hippocampal / Q000187:drug effects* / Q000473:pathology* / Q000528:radiation effects; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D018840:HSP70 Heat-Shock Proteins / Q000097:blood; D007030:Hypothalamo-Hypophyseal System / Q000187:drug effects* / Q000528:radiation effects; D008872:Microwaves / Q000009:adverse effects*; D010913:Pituitary-Adrenal System / Q000187:drug effects* / Q000528:radiation effects; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D001615:beta-Endorphin / Q000097:blood / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D018840:HSP70 Heat-Shock Proteins; C526717:qindan; D001615:beta-Endorphin; D000324:Adrenocorticotropic Hormone; D003346:Corticotropin-Releasing Hormone