In Situ Gene Expression Responsible for Sulfide Oxidation and CO2 Fixation of an Uncultured Large Sausage-Shaped Aquificae Bacterium in a Sulfidic Hot Spring.
We investigated the in situ gene expression profile of sulfur-turf microbial mats dominated by an uncultured large sausage-shaped Aquificae bacterium, a key metabolic player in sulfur-turfs in sulfidic hot springs. A reverse transcription-PCR analysis revealed that the genes responsible for sulfide, sulfite, and thiosulfate oxidation and carbon fixation via the reductive TCA cycle were continuously expressed in sulfur-turf mats taken at different sampling points, seasons, and years. These results suggest that the uncultured large sausage-shaped bacterium has the ability to grow chemolithoautotrophically and plays key roles as a primary producer in the sulfidic hot spring ecosystem in situ.
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST).
Satoshi
S
Tamazawa
Kyosuke
K
Yamamoto
Kazuto
K
Takasaki
Yasuo
Y
Mitani
Satoshi
S
Hanada
Yoichi
Y
Kamagata
Hideyuki
H
Tamaki
D001419:Bacteria / Q000145:classification* / Q000166:cytology / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism*; D052837:Chemoautotrophic Growth; D002952:Citric Acid Cycle; D020869:Gene Expression Profiling; D045482:Hot Springs / Q000382:microbiology*; D010084:Oxidation-Reduction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013440:Sulfides / Q000378:metabolism*; D013447:Sulfites / Q000378:metabolism; D013885:Thiosulfates / Q000378:metabolism
D013440:Sulfides; D013447:Sulfites; D013885:Thiosulfates; D002245:Carbon Dioxide