A Gold Nanoparticle Platform for the Delivery of Functional TGF-β1 siRNA Into Cancer Cells.
Nanoparticles, especially gold nanoparticles (AuNPs), have been shown to be an efficient carrier to deliver small RNAs into cancer cells. In this study, we used cysteamine-functionalized AuNPs to effectively deliver TGF-β1 siRNA into hepatoma HepG2 cells in vitro and in vivo. We found that, compared with AuNPs-mediated NC siRNA (AuNP-siNC), AuNPs-delivered TGF-β1 siRNA (AuNP-siTGFβ1) efficiently decreased the level of TGF-β1, increased cell apoptosis, and significantly inhibited the proliferation of recipient tumour cells. Systemic administration of the AuNP-siTGFβ1 complexes into human HepG2 xenografted mice likewise reduced TGF-β1 expression and downstream TGF-β1 signalling. Functionally, AuNP-siTGFβ1 strongly inhibited tumour growth and improved the survival rate of tumour-bearing mice compared with the control groups. In conclusion, our results demonstrate that the siRNA delivery system with AuNP described here appears to be a highly effective method to deliver RNAi therapeutics into tumour cells for oncotherapy.
Jindao
J
Wu
Bin
B
Liu
Heming
H
Wu
Younong
Y
Wu
Wei
W
Zhang
Shouwei
S
Zhao
Long
L
Zhang
Xiongxiong
X
Pan
Wen
W
Gao
Xuehao
X
Wang
Yi
Y
Yuan
Yaqin
Y
Zhang
D017209:Apoptosis / Q000235:genetics; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000235:genetics; D020868:Gene Silencing; D006046:Gold / Q000737:chemistry; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D058990:Molecular Targeted Therapy / Q000379:methods; D053769:Nanocapsules / Q000737:chemistry / Q000648:ultrastructure; D009374:Neoplasms, Experimental / Q000235:genetics* / Q000628:therapy*; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000235:genetics*; D014162:Transfection / Q000379:methods; D053773:Transforming Growth Factor beta1 / Q000235:genetics*; D016896:Treatment Outcome
D053769:Nanocapsules; D034741:RNA, Small Interfering; D053773:Transforming Growth Factor beta1; D006046:Gold