Mixed Zwitterion-Based Self-Assembled Monolayer Interface for Impedimetric Glycomic Analyses of Human IgG Samples in an Array Format.
An impedimetric lectin biosensor for the detection of changes in the glycan structure of antibodies isolated from human serum is here correlated with the progression of rheumatoid arthritis (RA). The biosensor was built up from a mixed self-assembled monolayer (SAM) on gold consisting of two different thiolated zwitterionic derivatives, carboxybetaine and sulfobetaine, to resist nonspecific interactions. The carboxyl-terminated one was applied also for the covalent immobilization of lectin Ricinus communis agglutinin I (RCA-I). The process of building a bioreceptive layer was optimized and characterized using a diverse range of techniques. Impedimetric assays were integrated on a chip consisting of eight gold working electrodes, which is an important step toward the achievement of a moderate level of multiplexing for the analysis of human serum samples. At the end, the results obtained by the impedimetric analysis of immunoglobulins G (IgGs) isolated from serum samples were compared with those of two other standard bioanalytical methods employing lectins, that is, lectin microarrays (MAs) and enzyme-linked lectin binding assays (ELLBAs). The impedimetric results agreed very well with the DAS28 index (RA disease activity score 28), suggesting that impedimetric assays could be used for the development of a new diagnostic procedure sensitive to glycosylation changes in human IgGs and thus RA progression.
Institute of Chemistry, Slovak Academy of Sciences , Dubravska cesta 9, Bratislava 845 38, Slovak Republic.
Tomas
T
Bertok
Institute of Chemistry, Slovak Academy of Sciences , Dubravska cesta 9, Bratislava 845 38, Slovak Republic.
Erika
E
Dosekova
Institute of Chemistry, Slovak Academy of Sciences , Dubravska cesta 9, Bratislava 845 38, Slovak Republic.
Stefan
S
Belicky
Institute of Chemistry, Slovak Academy of Sciences , Dubravska cesta 9, Bratislava 845 38, Slovak Republic.
Alena
A
Holazova
Institute of Chemistry, Slovak Academy of Sciences , Dubravska cesta 9, Bratislava 845 38, Slovak Republic.
Lenka
L
Lorencova
Institute of Chemistry, Slovak Academy of Sciences , Dubravska cesta 9, Bratislava 845 38, Slovak Republic.
Danica
D
Mislovicova
Institute of Chemistry, Slovak Academy of Sciences , Dubravska cesta 9, Bratislava 845 38, Slovak Republic.
Darina
D
Paprckova
Institute of Chemistry, Slovak Academy of Sciences , Dubravska cesta 9, Bratislava 845 38, Slovak Republic.
Alica
A
Vikartovska
BVT Technologies, Inc. , Hudcova 78c, Brno 612 00, Czech Republic.
Robert
R
Plicka
BVT Technologies, Inc. , Hudcova 78c, Brno 612 00, Czech Republic.
Jan
J
Krejci
Center for Advanced Materials, Qatar University , P.O. Box 2713 Doha, Qatar.
Marketa
M
Ilcikova
Center for Advanced Materials, Qatar University , P.O. Box 2713 Doha, Qatar.
Peter
P
Kasak
Institute of Chemistry, Slovak Academy of Sciences , Dubravska cesta 9, Bratislava 845 38, Slovak Republic.
Jan
J
Tkac
D001172:Arthritis, Rheumatoid / Q000097:blood*; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D006031:Glycosylation; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D007074:Immunoglobulin G / Q000032:analysis* / Q000097:blood; D037121:Plant Lectins / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis* / Q000097:blood; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods
D007074:Immunoglobulin G; D037121:Plant Lectins; D011134:Polysaccharides; C017707:Ricinus communis agglutinin-1