Veterinary Hospital Dissemination of CTX-M-15 Extended-Spectrum Beta-Lactamase-Producing Escherichia coli ST410 in the United Kingdom.
We characterized extended-spectrum beta-lactamases (ESBLs) and plasmid-mediated quinolone resistance (PMQR) in 32 Escherichia coli extended spectrum cephalosporin (ESC)-resistant clinical isolates from UK companion animals from several clinics. In addition, to investigate the possible dissemination of ESBL clinical isolates within a veterinary hospital, two ESBL-producing E. coli isolates from a dog with septic peritonitis and a cluster of environmental ESC-resistant E. coli isolates obtained from the same clinic and during the same time period, as these two particular ESBL-positive clinical isolates, were also included in the study. Molecular characterization identified blaCTX-Mto be the most prevalent gene in ESC-resistant isolates, where 66% and 27% of clinical isolates carried blaCTX-M-15and blaCTX-M-14,respectively. The only PMQR gene detected was aac(6')-Ib-cr, being found in 34% of the ESC E. coli isolates and was associated with the carriage of blaCTX-M-15. The clinical and environmental isolates investigated for hospital dissemination had a common ESBL/AmpC phenotype, carried blaCTX-M-15, and co-harbored blaOXA-1,blaTEM-1,blaCMY-2,and aac(6')-Ib-cr. Multilocus sequence typing identified them all as ST410, while pulse-field gel electrophoresis demonstrated 100% homology of clinical and environmental isolates, suggesting hospital environmental dissemination of CTX-M-15-producing E. coli ST410.
1 School of Veterinary Science, University of Liverpool , Liverpool, United Kingdom .|2 Department of Infection Biology, Institute of Infection and Global Health, University of Liverpool , Liverpool, United Kingdom .|3 Faculty of Veterinary Medicine, University of Agronomical Sciences and Veterinary Medicine , Iasi, Romania .
Dorina
D
Timofte
1 School of Veterinary Science, University of Liverpool , Liverpool, United Kingdom .
Iuliana Elena
IE
Maciuca
4 Department of Epidemiology and Population Health, Institute of Infection and Global Health, University of Liverpool , Liverpool, United Kingdom .
Nicola J
NJ
Williams
1 School of Veterinary Science, University of Liverpool , Liverpool, United Kingdom .
Andrew
A
Wattret
1 School of Veterinary Science, University of Liverpool , Liverpool, United Kingdom .|2 Department of Infection Biology, Institute of Infection and Global Health, University of Liverpool , Liverpool, United Kingdom .
Vanessa
V
Schmidt
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002371:Cat Diseases / Q000453:epidemiology* / Q000382:microbiology / Q000635:transmission; D002415:Cats; D018550:Cephalosporin Resistance / Q000235:genetics*; D002511:Cephalosporins / Q000494:pharmacology; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology / Q000635:transmission / Q000662:veterinary*; D004283:Dog Diseases / Q000453:epidemiology* / Q000382:microbiology / Q000635:transmission; D004285:Dogs; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology / Q000635:transmission / Q000662:veterinary*; D015870:Gene Expression; D005838:Genotype; D006762:Hospitals, Animal; D008826:Microbial Sensitivity Tests; D058885:Multilocus Sequence Typing; D057805:Pets / Q000382:microbiology; D010641:Phenotype; D006113:United Kingdom / Q000453:epidemiology; D001618:beta-Lactamases / Q000235:genetics*
D000900:Anti-Bacterial Agents; D002511:Cephalosporins; C473109:beta-lactamase CTX-M-15; D001618:beta-Lactamases
E. coli; ESBL; infection control; surveillance; veterinary