SiRNA-mediated silencing of Snail-1 induces apoptosis and alters micro RNA expression in human urinary bladder cancer cell line.
Snail-1 known as one of the important transcription factor is a mediator of survival and cell migration, and expression is raised in numerous cancer types. Snail-1 gene may show a role in recurrence of several cancers including bladder cancer by down-regulating E-cadherin, inducing an epithelial to mesenchymal transition (EMT) and its related microRNAs (miRNAs). The aim of this study was to investigate the effect of a specific Snail-1 siRNA on apoptosis and alter EMT related miRNAs of EJ-138 (bladder cancer) cells. The cells were transfected with siRNAs using transfection reagent. The cytotoxic effects of Snail-1 siRNA, on bladder cancer cells were determined using MTT assay. Relative Snail-1 mRNA levels were measured by QRT- PCR, respectively. Apoptosis was measured by TUNEL test based on labeling of DNA strand breaks. We also evaluated miR-29b, miR-21, and miR-203 expression by QRT-PCR to determine alteration in miRNAs expression involved in EMT. Snail-1 siRNA significantly reduced mRNA expression levels in 48 h after transfection at the concentration of 60 pmol in bladder cancer cells. We also showed that the silencing of Snail-1 led to the induction of apoptosis. miR-21 and miR-29b depression have been shown in Snail-1 suppressed group in EJ-138 cells in vitro. These results propose that Snail-1 might play an important role in the progression of bladder cancer, and be a potential therapeutic target for trigger apoptosis and suppression of EMT-related miRNAs in bladder cancer.
a Immunology Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.|b International Branch (Aras), Tabriz University of Medical Sciences , Tabriz , Iran.
Seyed Mohammad Hossein
SMH
Musavi Shenas
a Immunology Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.|c Student Research Committee, Tabriz University of Medical Sciences , Tabriz , Iran.
Behzad
B
Mansoori
a Immunology Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.|c Student Research Committee, Tabriz University of Medical Sciences , Tabriz , Iran.
Ali
A
Mohammadi
a Immunology Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.
Shima
S
Salehi
a Immunology Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.
Behzad
B
Kaffash
a Immunology Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.
Behnaz
B
Talebi
d Department of Immunology , Tabriz University of Medical Sciences , Tabriz , Iran.
Zohreh
Z
Babaloo
a Immunology Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.
Dariush
D
Shanehbandi
a Immunology Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.
Behzad
B
Baradaran
D017209:Apoptosis / Q000235:genetics*; D015820:Cadherins / Q000235:genetics; D045744:Cell Line, Tumor; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D020868:Gene Silencing; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D034741:RNA, Small Interfering / Q000235:genetics*; D000071250:Snail Family Transcription Factors / Q000172:deficiency / Q000235:genetics*; D001749:Urinary Bladder Neoplasms / Q000473:pathology*
D015820:Cadherins; C524940:MIRN21 microRNA, human; C532045:MIRN29a microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering; C000604407:SNAI1 protein, human; D000071250:Snail Family Transcription Factors
Apoptosis; EMT; bladder cancer; micro RNA; small interference RNA; snail-1