Effects of Guchang Capsule on Dextran Sulphate Sodium-Induced Experimental Ulcerative Colitis in Mice.
Guchang capsule (GC) is a Chinese materia medica standardized product extracted from 15 Chinese traditional medical herbs and it has been clinically used in the treatment of intestinal disease. In this study, in order to extend the research of GC in intestinal disease, we were aiming to evaluate potential effects of GC on dextran sulphate sodium- (DSS-) induced murine experimental colitis and to elucidate the underlying mechanisms. GC treatment attenuated DSS-induced body weight loss and reduced the mortality. Moreover, GC treatment prevented DSS-induced colonic pathological damage; meanwhile it inhibited proinflammatory cytokines production in colon tissues. In vitro, GC significantly reduced LPS-induced proinflammatory cytokines production via inhibiting the activation of NF-κB in macrophage cells, and the expressions of several long noncoding RNAs (lncRNAs) which were reported in regulating NF-κB signaling pathway were obviously affected by adding GC into culture medium. In conclusion, our data suggested that administration of GC exhibits therapeutic effects on DSS-induced colitis partially through regulating the expression of NF-κB related lncRNAs in infiltrating immune cells.
Department of Traditional Chinese Medicine, Tianjin Medical University General Hospital, Tianjin 300052, China.
Baoshan
B
Liu
Tianjin Zhongxin Pharmaceutical Group Corporation LTD., Tianjin 300457, China.
Tong
T
Liu
Department of Traditional Chinese Medicine, Tianjin Medical University General Hospital, Tianjin 300052, China.
Xiaohong
X
Wang
Tianjin Zhongxin Pharmaceutical Group Corporation LTD., Tianjin 300457, China.
Xin
X
Zheng
Department of Traditional Chinese Medicine, Tianjin Medical University General Hospital, Tianjin 300052, China.
Hong
H
Wang
Department of Traditional Chinese Medicine, Tianjin Medical University General Hospital, Tianjin 300052, China.
Lin
L
Ma