Chicken cecal microRNAs in the response to Campylobacter jejuni inoculation by Solexa sequencing.
Campylobacter jejuni (C. jejuni) is one of major foodborne pathogen that cause human diarrhea by consuming C. jejuni contaminated chicken products. MicroRNAs play an integral role in many different biological processes including bacteria and virus inoculation in chickens. In this study, we identified chicken miRNAs responding to C. jejuni inoculation through Solexa sequencing in the cecum. As a result, four miRNAs were significantly differentially expressed between inoculated and non-inoculated groups. There were 1,114 putative target genes regulated by those differentially expressed miRNAs predicted by miRanda, TargetScan, and miRTarget softwares. Functional analysis of those target genes showed that 113 gene ontology biological process terms and 14 pathways were significantly enriched. Hedgehog signaling pathway may contribute to chicken C. jejuni inoculation. MiR-155 played vital role in the C. jejuni inoculation. The result herein will lay the foundation for the further study of regulatory mechanism of chicken miRNAs in the response to C. jejuni inoculation.
College of Animal Science, Shandong Agricultural University, Taian 271018, China.
Xiaoyi
X
Liu
College of Life Science, Shandong Agricultural University, Taian 271018, China.
Liying
L
Liu
College of Animal Science, Shandong Agricultural University, Taian 271018, China.
Maozhi
M
Zhang
College of Animal Science, Shandong Agricultural University, Taian 271018, China.
Huicui
H
Wang
College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Ning
N
Yang
College of Animal Science, Shandong Agricultural University, Taian 271018, China xyli@sdau.edu.cn.|Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention; Tai'an 271018, China.
Xianyao
X
Li
D000818:Animals; D002169:Campylobacter Infections / Q000378:metabolism / Q000382:microbiology / Q000662:veterinary*; D016123:Campylobacter jejuni; D002432:Cecum / Q000378:metabolism / Q000382:microbiology*; D002645:Chickens / Q000382:microbiology; D015870:Gene Expression; D035683:MicroRNAs / Q000302:isolation & purification / Q000378:metabolism*; D011201:Poultry Diseases / Q000378:metabolism / Q000382:microbiology*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary
D035683:MicroRNAs
Campylobacter jejuni; chicken; expression; miRNA