Comparative toxicity profiles of Plumbago zeylanica L. root petroleum ether, acetone and hydroalcoholic extracts in Wistar rats.
BACKGROUND
The root of Plumbago zeylanica Linn. is used in traditional medicine for the treatment of chronic inflammatory diseases and various disorders. The toxicity of this plant has not yet been extensively evaluated.


OBJECTIVE
To evaluate and compare the toxicity of P. zeylanica root petroleum ether (PZPE), acetone (PZAC), and the hydroalcoholic (PZHY) extracts.


METHODS
The acute and sub-acute toxicities of extracts were evaluated according to OECD guidelines 425 and 407, respectively in female rats.


RESULTS
PZPE was more toxic than PZAC and PZHA, based on LD50 values of 93.45, 928.4, and 928.4 mg/kg, respectively. This potency difference directly correlates with the plumbagin content of extracts. With regard to sub-acute toxicity, a significant increase in organ weights (liver, adrenal glands, and/or heart) was observed in PZPE and PZAC treated groups. All extracts produced a significant increase in serum aspartate aminotransferase and urea, and PZAC produced a significant increase in serum creatinine as compared to control. A decrease in hematocrit was observed in the highest dose PZPE group, and a decrease in leukocytes was observed in all PZAC groups. Hepatic and renal changes were observed in all extract treated groups.


CONCLUSIONS
The findings of our study, thus demonstrate that liver and kidney are the primary organs being adversely affected following sub-acute administration of P. zeylanica root extract in rats.
Regional Medical Research Centre (ICMR), Belgaum, India.
Dushyant
D
Kumar
Department of Pharmacology, USM-KLE, Belgaum, Karnataka, India.
Paragouda A
PA
Patil
Regional Medical Research Centre (ICMR), Belgaum, India.
Subarna
S
Roy
Regional Medical Research Centre (ICMR), Belgaum, India.
Sanjiv D
SD
Kholkute
Regional Medical Research Centre (ICMR), Belgaum, India.
Harsha V
HV
Hegde
IPR Division-05, Evalueserve SEZ (Gurgaon) Pvt. Ltd., Gurgaon, New Delhi, India.
Vinod
V
Nair
Acute toxicity; Plumbago zeylanica; nephrotoxicity; plumbagin; sub-acute toxicity