Calcium Signaling of Lysophosphatidylethanolamine through LPA1in Human SH-SY5Y Neuroblastoma Cells.
Lysophosphatidylethanolamine (LPE), a lyso-type metabolite of phosphatidylethanolamine, has been reported to be an intercellular signaling molecule. LPE mobilizes intracellular Ca2+through G-protein-coupled receptor (GPCR) in some cells types. However, GPCRs for lysophosphatidic acid (LPA) were not implicated in the LPE-mediated activities in LPA GPCR overexpression systems or in SK-OV3 ovarian cancer cells. In the present study, in human SH-SY5Y neuroblastoma cells, experiments with LPA1antagonists showed LPE induced intracellular Ca2+increases in an LPA1GPCR-dependent manner. Furthermore, LPE increased intracellular Ca2+through pertussis-sensitive G proteins, edelfosine-sensitive-phospholipase C, 2-APB-sensitive IP3receptors, Ca2+release from intracellular Ca2+stores, and subsequent Ca2+influx across plasma membranes, and LPA acted on LPA1and LPA2receptors to induce Ca2+response in a 2-APB-sensitive and insensitive manner. These findings suggest novel involvements for LPE and LPA in calcium signaling in human SH-SY5Y neuroblastoma cells.
Molecular Inflammation Research Center for Aging Intervention (MRCA) and College of Pharmacy, Pusan National University, Busan 46241, Republic of Korea.
Jung-Min
JM
Lee
Molecular Inflammation Research Center for Aging Intervention (MRCA) and College of Pharmacy, Pusan National University, Busan 46241, Republic of Korea.
Soo-Jin
SJ
Park
Molecular Inflammation Research Center for Aging Intervention (MRCA) and College of Pharmacy, Pusan National University, Busan 46241, Republic of Korea.
Dong-Soon
DS
Im
GPCR; LPA₁; Lysophosphatidic acid; Lysophosphatidylethanolamine; Neuroblastoma; Receptor