The Characterization of AT1Expression in the Dorsal Root Ganglia After Chronic Constriction Injury.
To clarify the role of Angiotensin II in the regulation of sensory signaling, we characterized the AT1expression in neuronal subpopulation of lower lumbar dorsal root ganglia under normal conditions and its alteration in neuropathic pain model. The characterization of AT1expression was done under control and after the chronic constriction injury induced by four loose ligatures of the sciatic nerve representing the model of posttraumatic painful peripheral neuropathy. Major Angiotensin II receptor type was expressed in approximately 43 % of small-sized and 62 % of large-sized neurons in control. The AT1overexpression after sciatic nerve ligation lasting 7 days was detected predominantly in small-sized AT1immunoreactive neurons (about 38 % increase). Chronic constriction injury caused a statistically marked increase in number of the small-sized peptidergic (CGRP immunoreactive) neuronal subpopulation expressing AT1(about 64 %). The subpopulations of AT1-immunoreactive and nonpeptide-containing primary sensory neurons revealed by IB4 binding, tyrosine hydroxylase- and parvalbumin-immunoreactive neurons were not markedly changed. Our results indicate that: (1) the AT1overexpression after the chronic constriction injury is an important factor in Angiotensin II-potentiated pain perception; (2) Angiotensin II is involved in pathological mechanisms of neuropathic pain and this effect can be mediated perhaps in combination with other neuropeptides synthesized in the primary sensory neurons.
Department of Neurodegeneration, Plasticity and Repair, Institute of Neurobiology, Slovak Academy of Sciences, Soltesovej 4-6, 040 01, Kosice, Slovak Republic.
Zuzana
Z
Oroszova
Department of Neurodegeneration, Plasticity and Repair, Institute of Neurobiology, Slovak Academy of Sciences, Soltesovej 4-6, 040 01, Kosice, Slovak Republic.
Ludmila
L
Hricova
Department of Neurodegeneration, Plasticity and Repair, Institute of Neurobiology, Slovak Academy of Sciences, Soltesovej 4-6, 040 01, Kosice, Slovak Republic.
Andrea
A
Stropkovska
Department of Neurodegeneration, Plasticity and Repair, Institute of Neurobiology, Slovak Academy of Sciences, Soltesovej 4-6, 040 01, Kosice, Slovak Republic.
Nadezda
N
Lukacova
Department of Neurodegeneration, Plasticity and Repair, Institute of Neurobiology, Slovak Academy of Sciences, Soltesovej 4-6, 040 01, Kosice, Slovak Republic. pavel@saske.sk.
Jaroslav
J
Pavel
D000804:Angiotensin II / Q000378:metabolism; D000818:Animals; D000918:Antibody Specificity / Q000276:immunology; D015153:Blotting, Western; D002478:Cells, Cultured; D003250:Constriction; D005455:Fluorescent Antibody Technique; D005727:Ganglia, Spinal / Q000378:metabolism* / Q000473:pathology*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D008159:Lumbar Vertebrae / Q000473:pathology; D008297:Male; D009457:Neuroglia / Q000378:metabolism; D017208:Rats, Wistar; D044140:Receptor, Angiotensin, Type 1 / Q000276:immunology / Q000378:metabolism*; D012584:Sciatic Nerve / Q000293:injuries* / Q000378:metabolism* / Q000473:pathology; D011984:Sensory Receptor Cells / Q000473:pathology
D005904:Glial Fibrillary Acidic Protein; D044140:Receptor, Angiotensin, Type 1; D000804:Angiotensin II
AT1; Angiotensin II; Calcitonin gene-related peptide; Chronic constriction injury; Dorsal root ganglia; Neuropathic pain