Bromfenac ophthalmic solution 0.09 %: human aqueous humor concentration detected by high-performance liquid chromatography.
The purpose of this study was to evaluate the aqueous humor concentrations of bromfenac ophthalmic solution 0.09 % in patients undergoing phacoemulsification. Patients requiring cataract extraction received one drop (50 µL) of bromfenac 0.09 % solution in the eye to be operated, before bedtime the day before surgery or the morning of the surgery. The last administration was recorded. At the time of paracentesis, an aqueous humor sample was collected with a 30-gauge needle attached to a TB syringe and was later analyzed by high-performance liquid chromatography for drug concentration. 188 treated volunteers and 48 control, untreated, subjects were included in the study. The mean aqueous concentration of bromfenac in the treated group was 37.60 ± 68.86 and 0 nM (nmol/L) in the control group (p < 0.0001). Correlation coefficient in bromfenac group between time elapsed from instillation and drug concentration was -0.16 (p not significant). Bromfenac showed properties of good penetration and stable concentration in aqueous humor up to about 12 h after instillation.
Department of Neuroscience, Rehabilitation, Ophthalmology, Genetics, Maternal and Child Health, University of Genoa, Via San Luca d'Albaro 37/1, 16146, Genoa, Italy. macria70@gmail.com.
Angelo
A
Macrì
Department of Neuroscience, Rehabilitation, Ophthalmology, Genetics, Maternal and Child Health, University of Genoa, Via San Luca d'Albaro 37/1, 16146, Genoa, Italy.
Aldo
A
Vagge
Center of Excellence for Biomedical Research (CEBR), University of Genoa, Viale Benedetto XV 5, 16132, Genoa, Italy.
Annalisa
A
Salis
Department of Internal Medicine, Pharmacology and Toxicology Unit, University of Genoa, Genoa, Italy.
Carmen
C
Fucile
Department of Internal Medicine, Pharmacology and Toxicology Unit, University of Genoa, Genoa, Italy.
Valeria
V
Marini
Department of Internal Medicine, Pharmacology and Toxicology Unit, University of Genoa, Genoa, Italy.
Antonietta
A
Martelli
Bausch + Lomb Iom SpA, Viale Martesana 12, 20090, Vimodrone, MI, Italy.
Sebastiano
S
Giuffrida
Department of Neuroscience, Rehabilitation, Ophthalmology, Genetics, Maternal and Child Health, University of Genoa, Via San Luca d'Albaro 37/1, 16146, Genoa, Italy.
Michele
M
Iester
Center of Excellence for Biomedical Research (CEBR), University of Genoa, Viale Benedetto XV 5, 16132, Genoa, Italy.|Department of Experimental Medicine, Section of Biochemistry, University of Genoa, Viale Benedetto XV 1, 16132, Genoa, Italy.
Gianluca
G
Damonte
Department of Internal Medicine, Pharmacology and Toxicology Unit, University of Genoa, Genoa, Italy.
Francesca
F
Mattioli
D000368:Aged; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000032:analysis / Q000493:pharmacokinetics; D001082:Aqueous Humor / Q000737:chemistry*; D001577:Benzophenones / Q000008:administration & dosage / Q000032:analysis* / Q000493:pharmacokinetics; D001969:Bromobenzenes / Q000008:administration & dosage / Q000032:analysis* / Q000493:pharmacokinetics; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D009883:Ophthalmic Solutions / Q000008:administration & dosage / Q000493:pharmacokinetics; D018918:Phacoemulsification; D011300:Preoperative Care; D011446:Prospective Studies; D013997:Time Factors
D000894:Anti-Inflammatory Agents, Non-Steroidal; D001577:Benzophenones; D001969:Bromobenzenes; D009883:Ophthalmic Solutions; C053083:bromfenac
Bromfenac; Humor aqueous concentration; Ocular instillation