Therapeutic Effect of Intrastromal Voriconazole, Topical Voriconazole, and Topical Natamycin on Fusarium Keratitis in Rabbit.
Purpose. Evaluating the therapeutic effect of topical and intrastromal voriconazole and topical natamycin on Fusarium keratitis. Methods. 24 rabbits were selected. The stroma of their corneas was inoculated with suspension of Fusarium solani species complex. Seven days after injection they were divided into 4 groups randomly: the first group was treated with topical voriconazole (TV) 1% for one week, the second one with one-time intrastromal injection of voriconazole (ISV) 50 microgram/0.1 mL, and the third group with topical gel of natamycin (TN) for one week, and the last one did not receive any antifungal treatment. Finally the eyes were enucleated and sclerocorneal buttons were sent for histological and microbiological examinations. Results. After treatment the ISV group and TN group showed significantly lower clinical score and colony forming units than the control group (P = 0.040 and P = 0.026, resp.), but there was statistically no significant difference between control and TV groups (P = 0.249) or between ISV and TN groups (P = 0.665). In pathological evaluation, fewer chronic inflammations were reported in 2 of the 3 buttons from TV group and 3 of the 3 buttons from ISV and TN groups in comparison with the control group. Conclusion. Intrastromal injection of voriconazole seems to be effective in treatment of Fusarium keratitis as much as topical natamycin and these are more effective than topical voriconazole.
Poostchi Eye Research Center, Department of Ophthalmology, Shiraz University of Medical Sciences, Shiraz 7134845794, Iran.
Mahmood
M
Nejabat
Department of Ophthalmology, Shiraz University of Medical Sciences, Shiraz 7134845794, Iran.
Nafiseh
N
Yaqubi
Yasuj University of Medical Sciences, Yasuj 7591741417, Iran.
Amir
A
Khosravi
Basic Sciences in Infectious Diseases Research Center and Department of Medical Mycology and Parasitology, School of Medicine, Shiraz University of Medical Sciences, Shiraz 7134845794, Iran.
Kamiar
K
Zomorodian
Department of Pathology, Shiraz University of Medical Sciences, Shiraz 7134845794, Iran.
Mohammad Javad
MJ
Ashraf
Poostchi Eye Research Center, Department of Ophthalmology, Shiraz University of Medical Sciences, Shiraz 7134845794, Iran.
Ramin
R
Salouti