ELP-OPH/BSA/TiO2 nanofibers/c-MWCNTs based biosensor for sensitive and selective determination of p-nitrophenyl substituted organophosphate pesticides in aqueous system.
A novel biosensor for rapid, sensitive and selective monitoring of p-nitrophenyl substituted organophosphate pesticides (OPs) in aqueous system was developed using a functional nanocomposite which consists of elastin-like-polypeptide-organophosphate hydrolase (ELP-OPH), bovine serum albumin (BSA), titanium dioxide nanofibers (TiO2NFs) and carboxylic acid functionalized multi-walled carbon nanotubes (c-MWCNTs). ELP-OPH was simply purified from genetically engineered Escherichia coli based on the unique phase transition of ELP and thus served as biocatalyst for OPs, while BSA was used to stabilize OPH activity in the nanocomposite. TiO2NFs was employed to enrich organophosphates in the nanocomposite due to its strong affinity with phosphoric group in OPs, while c-MWCNTs was used to enhance the electron transfer in the amperometric detection as well as for covalent immobilization of ELP-OPH. ELP-OPH/BSA/TiO2NFs/c-MWCNTs nanocomposite were systematically characterized using field emission scanning electron microscopy (SEM), Raman spectra, Fourier Transform infrared spectroscopy (FTIR) and X-ray Diffraction (XRD). Under the optimized operating conditions, the ELP-OPH/BSA/TiO2NFs/c-MWCNTs based biosensor for OPs shows a wide linear range, a fast response (less than 5s) and limits of detection (S/N=3) as low as 12nM and 10nM for methyl parathion and parathion, respectively. Such excellent sensing performance can be attributed to the synergistic effects of the individual components in the nanocomposite. Its further application for selectively monitoring OPs compounds spiked in lake water samples was also demonstrated with good accuracy. These features indicate that the developed nanocomposite offers an excellent biosensing platform for rapid, sensitive and selective detection of organophosphates compounds.
Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400030, China; Department of Biomedical Engineering, University of Connecticut, Storrs, CT 06269, USA.
Jing
J
Bao
Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400030, China. Electronic address: houcj@cqu.edu.cn.
Changjun
C
Hou
Department of Biomedical Engineering, University of Connecticut, Storrs, CT 06269, USA.
Qiuchen
Q
Dong
Department of Biomedical Engineering, University of Connecticut, Storrs, CT 06269, USA.
Xiaoyu
X
Ma
Department of Biomedical Engineering, University of Connecticut, Storrs, CT 06269, USA.
Jun
J
Chen
Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400030, China.
Danqun
D
Huo
Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400030, China.
Mei
M
Yang
Department of Microbiology, Faculty of Pharmacy, Mansoura University, Egypt.
Khaled Hussein Abd El
KHAE
Galil
Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE 19716, USA.
Wilfred
W
Chen
Department of Biomedical Engineering, University of Connecticut, Storrs, CT 06269, USA; Department of Chemical and Biomolecular Engineering, University of Connecticut, Storrs, CT 06269, USA. Electronic address: ylei@engr.uconn.edu.
Yu
Y
Lei
D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D004549:Elastin / Q000737:chemistry / Q000235:genetics; D055664:Electrochemical Techniques / Q000379:methods; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005818:Genetic Engineering; D060106:Lakes / Q000032:analysis; D008743:Methyl Parathion / Q000032:analysis; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D010755:Organophosphates / Q000032:analysis*; D010278:Parathion / Q000032:analysis; D010455:Peptides / Q000737:chemistry / Q000235:genetics; D010575:Pesticides / Q000032:analysis*; D010744:Phosphoric Monoester Hydrolases / Q000737:chemistry / Q000235:genetics; D012710:Serum Albumin, Bovine / Q000737:chemistry; D014025:Titanium / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis*
D037742:Nanotubes, Carbon; D010755:Organophosphates; D010455:Peptides; D010575:Pesticides; D014874:Water Pollutants, Chemical; C009495:titanium dioxide; D012710:Serum Albumin, Bovine; D008743:Methyl Parathion; D010278:Parathion; D004549:Elastin; D014025:Titanium; C045290:phosphorylphosphatase; D010744:Phosphoric Monoester Hydrolases
ELP-OPH; Methyl parathion; Multi-walled carbon nanotubes; Parathion; Titanium dioxide nanofibers