Quantitative Assessment of the Effects of Oxidants on Antigen-Antibody Binding In Vitro.
Objective. We quantitatively assessed the influence of oxidants on antigen-antibody-binding activity. Methods. We used several immunological detection methods, including precipitation reactions, agglutination reactions, and enzyme immunoassays, to determine antibody activity. The oxidation-reduction potential was measured in order to determine total serum antioxidant capacity. Results. Certain concentrations of oxidants resulted in significant inhibition of antibody activity but had little influence on total serum antioxidant capacity. Conclusions. Oxidants had a significant influence on interactions between antigen and antibody, but minimal effect on the peptide of the antibody molecule.
College of Medical Laboratory, Dalian Medical University, Dalian 116044, China.
Shuang
S
Han
Second Affiliated Hospital of Dalian Medical University, Dalian 116023, China.
Guanyu
G
Wang
College of Medical Laboratory, Dalian Medical University, Dalian 116044, China.
Naijin
N
Xu
College of Medical Laboratory, Dalian Medical University, Dalian 116044, China.
Hui
H
Liu
D000328:Adult; D000371:Agglutination; D000914:Antibodies, Viral / Q000276:immunology; D000936:Antigen-Antibody Complex / Q000737:chemistry / Q000378:metabolism*; D000975:Antioxidants / Q000032:analysis; D055664:Electrochemical Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006514:Hepatitis B Surface Antigens / Q000276:immunology; D006801:Humans; D047468:Immunoprecipitation; D008297:Male; D016877:Oxidants / Q000737:chemistry / Q000378:metabolism*
D000914:Antibodies, Viral; D000936:Antigen-Antibody Complex; D000975:Antioxidants; D006514:Hepatitis B Surface Antigens; D016877:Oxidants