Correlating blood levels of 8-hydroxydeoxyguanosine to hOGG1 genotypes and the incidence of ischemic cardiomyopathy.
We measured the serum levels of 8-hydroxydeoxyguanosine (8-OHdG) and investigated whether these levels correlate with incidence of ischemic cardiomyopathy (ICM), and whether these levels correlate with underlying oxidative stress in patients with ICM. Polymerase chain reaction-restriction fragment length polymorphism analysis was performed to assess the prevalence of the Ser/Cys polymorphism in the human 8-oxoguanine glycosylase (hOGG1) gene. We analyzed the samples from 246 ICM cases (the ICM group) and another 246 age- and sex-matched volunteers with normal coronary artery function (the control group). Levels of 8-OHdG in participants' blood samples were 6.7 ± 1.7 and 3.0 ± 0.8 in the ICM and control groups, respectively (p < 0.05). Although there were no differences in allele frequency (p = 0.140), significant differences were present in the genotype distributions (p = 0.002). The Cys/Cys genotype correlated strongly with the risk of developing ICM (odds ratio, 2.2; 95% confidence interval, 1.4-3.3). Treating the Ser/Ser and Ser/Cys genotypes as members of the same group increased the predicted ICM risk for patients carrying the Cys/Cys genotype (odds ratio, 1.9; 95% confidence interval, 1.2-2.9). The serum level of 8-OHdG in the ICM group was higher than that in the control group (p < 0.05) and significantly increased in those carrying the Cys/Cys genotype (8.7 ± 1.7 for the Cys/Cys group, and 4.5 ± 0.8 for the Ser/Ser+Ser/Cys group; p < 0.05). Patients carrying the Cys/Cys genotype had a significantly increased risk of developing ICM. Serum levels of 8-OHdG were significantly increased in patients with ICM.
Department of Cardiology, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
Yu
Y
Jin
Department of Cardiology, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. Electronic address: chunguangqiudc@163.com.
Chun-Guang
CG
Qiu
Department of Cardiology, Tangdu Hospital of the 4th Military Medical University, Xi'an 700038, China.
Qiang-Sun
QS
Zheng
Department of Cardiology, Wuhan General Hospital of Guangzhou Military Command, Wuhan 430070, China.
Shi-Fang
SF
Ding
Department of Cardiology, Wuhan General Hospital of Guangzhou Military Command, Wuhan 430070, China.
Qi-Jun
QJ
Jiang
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000368:Aged; D002097:C-Reactive Protein / Q000378:metabolism; D009202:Cardiomyopathies / Q000097:blood* / Q000201:enzymology / Q000235:genetics*; D016022:Case-Control Studies; D045647:DNA Glycosylases / Q000097:blood / Q000235:genetics*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000097:blood; D004587:Electrophoresis, Agar Gel; D005260:Female; D005340:Fibrinogen / Q000378:metabolism; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease; D006801:Humans; D015994:Incidence; D008055:Lipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D017202:Myocardial Ischemia / Q000097:blood* / Q000201:enzymology / Q000235:genetics*; D012307:Risk Factors
D008055:Lipids; D000080242:8-Hydroxy-2'-Deoxyguanosine; D005340:Fibrinogen; D002097:C-Reactive Protein; D045647:DNA Glycosylases; C479058:oxoguanine glycosylase 1, human; D003849:Deoxyguanosine
8-hydroxy guanine glycosylase; 8-hydroxy-deoxyguanosine; Ischemic cardiomyopathy; Oxidative stress; Polymorphism