The Effect of a High-Protein Diet and Exercise on Cardiac AQP7 and GLUT4 Gene Expression.
High-protein (HP) diets are commonly consumed by athletes despite their potential health hazard, which is postulated to enforce a negative effect on bone and renal health. However, its effects on heart have not been known yet. Aquaporin-7 (AQP7) is an aquaglyceroporin that facilitates glycerol and water transport. Glycerol is an important cardiac energy production substrate, especially during exercise, in conjunction with fatty acids and glucose. Glucose transporter 4 (GLUT4) is an insulin-sensitive glucose transporter in heart. We aimed to investigate the effect of HPD on AQP7 and GLUT4 levels in the rat heart subjected to exercise. Male Sprague-Dawley rats were divided into control (n = 12), exercise (E) training (n = 10), HPD (n = 12), and HPD-E training (n = 9) groups. The HPD groups were fed a 45 % protein-containing diet 5 weeks. The HPD-E and E groups were performed the treadmill exercise during the 5-week study period. Real-time polymerase chain reaction and immunohistochemistry techniques were used to determine the gene expression and localization of AQP7 and GLUT4 in heart tissue. Results of relative gene expression were calculated by the 'Pfaffl' mathematical method using the REST program. Differences in AQP7 and GLUT4 gene expression were expressed as fold change compared to the control group. Heart weight/tibia ratio and ventricular wall thickness were evaluated as markers of cardiac hypertrophy. Further, serum glucose, glycerol, and insulin levels were also measured. AQP7 gene expression was found to be increased in the E (3.47-fold, p < 0.001), HPD (5.59-fold, p < 0.001), and HPD-E (3.87-fold, p < 0.001) groups compared to the control group. AQP7 protein expression was also increased in the HPD and HPD-E groups (p < 0.001). Additionally, cardiac mRNA expression levels of GLUT4 showed a significant increase in the E (2.16-fold, p < 0.003), HPD (7.14-fold, p < 0.001), and HPD-E (3.43-fold, p < 0.001) groups compared to the control group. GLUT4 protein expression was significantly increased in the E, HPD, and HPD-E groups compared to the control group (p = 0.024, p < 0.001, and p < 0.001, respectively). Furthermore, Serum glucose levels were significantly different between groups (p < 0.005). This difference was observed between the HPD groups and normal-protein diet groups (C and E). Serum insulin levels were higher for HPD groups compared with the normal-protein diet groups (p < 0.001), whereas no differences were observed between the exercise and sedentary groups (p = 0.111). Serum glycerol levels were significantly increased in the HPD groups compared with control and E groups (p < 0.05 and p < 0.05, respectively). Consumption of HPD supplementation caused the increased effects on AQP7 and GLUT4 expression in rat heart.
Department of Biophysics, Faculty of Medicine, Trakya University, Edirne, Turkey. orkide_69@hotmail.com.
Orkide
http://orcid.org/0000-0002-3488-3740
O
Palabiyik
Department of Physiology, Faculty of Medicine, Trakya University, Edirne, Turkey.
Aziz
A
Karaca
Department of Pathology, Faculty of Medicine, Trakya University, Edirne, Turkey.
Ebru
E
Taştekin
Department of Biophysics, Faculty of Medicine, Trakya University, Edirne, Turkey.
Bilge Eren
BE
Yamasan
Department of Public Health, Faculty of Medicine, Trakya University, Edirne, Turkey.
Burcu
B
Tokuç
Department of Biophysics, Faculty of Medicine, Trakya University, Edirne, Turkey.
Tammam
T
Sipahi
Department of Physiology, Faculty of Medicine, Trakya University, Edirne, Turkey.
Selma Arzu
SA
Vardar
D000818:Animals; D020346:Aquaporins / Q000235:genetics* / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D004032:Diet / Q000009:adverse effects*; D005080:Exercise Test; D005786:Gene Expression Regulation; D051275:Glucose Transporter Type 4 / Q000235:genetics* / Q000378:metabolism; D005990:Glycerol / Q000097:blood; D007328:Insulin / Q000097:blood; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation
C491926:Aqp7 protein, rat; D020346:Aquaporins; D001786:Blood Glucose; D051275:Glucose Transporter Type 4; D007328:Insulin; C495694:Slc2a4 protein, rat; D005990:Glycerol
Aquaporin-7; Exercise; Glucose transporter 4; Glycerol; Heart; High-protein diet