Effects of Linderae radix extracts on a rat model of alcoholic liver injury.
Traditional treatments have a poor effect on alcoholic liver diseases. Linderae radix (LR), the dried root ofLindera aggregata(Sims) Kosterm., has been frequently used in traditional Chinese medicine for treating various diseases, and has been shown to exhibit a protective effect on liver injury. In the present study, LR extracts were made using various solvents, and then administrated to rats to establish a model of ethanol-induced liver injury. The study aimed to investigate the therapeutic effects and potential mechanism of LR extracts on acute alcoholic liver injury. The serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglycercide (TG), cholesterol (TC), methane dicarboxylic aldehyde (MDA) and superoxide dismutase (SOD) were determined using an automatic biochemistry analyzer. In addition, pathological examination was performed by hematoxylin-eosin staining. The levels of MDA and SOD, and the expression levels of nuclear factor (NF)-κB, tumor necrosis factor (TNF)-α and interleukin (IL)-1β in liver tissue were investigated immunohistochemically. The expression of cytochrome P450 2E1 (CYP2E1) mRNA was quantified by reverse transcription-quantitative polymerase chain reaction. The results indicated that LR extracts improved the histopathological status and decreased the serum levels of ALT, AST, TG, TC and MDA. Furthermore, the levels of MDA and inflammatory mediators (NF-κB, TNF-α and IL-1β) were decreased in liver tissues, and the overexpression of CYP2E1 mRNA induced by ethanol treatment. LR extracts exhibited a protective effect on alcoholic liver injury and the mechanism may be associated with the anti-inflammatory and anti-oxidative action.
Emergency Department, People's Hospital of Tiantai County, Tiantai, Zhejiang 317200, P.R. China.
Jun-Wei
JW
Wang
Emergency Department, People's Hospital of Tiantai County, Tiantai, Zhejiang 317200, P.R. China.
Xin-Yi
XY
Chen
Emergency Department, People's Hospital of Tiantai County, Tiantai, Zhejiang 317200, P.R. China.
Pei-Yang
PY
Hu
Emergency Department, People's Hospital of Tiantai County, Tiantai, Zhejiang 317200, P.R. China.
Ming-Ming
MM
Tan
Emergency Department, People's Hospital of Tiantai County, Tiantai, Zhejiang 317200, P.R. China.
Xiao-Gang
XG
Tang
Safety Evaluation Center, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang 310007, P.R. China.
Min-Cong
MC
Huang
Institute of Material Medica, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, P.R. China.
Zhao-Huan
ZH
Lou
Linderae radix; acute alcoholic liver injury; anti-inflammatory; anti-oxidant