Endothelial Progenitor Cells Combined with Cytosine Deaminase-Endostatin for Suppression of Liver Carcinoma.
Transplantation of gene transfected endothelial progenitor cells (EPCs) provides a novel method for treatment of human tumors. To study treatment of hepatocellular carcinoma using cytosine deaminase (CD)- and endostatin (ES)-transfected endothelial progenitor cells (EPCs), mouse bone marrow-derived EPCs were cultured and transfected with Lenti6.3-CD-EGFP and Lenti6.3-ES-Monomer-DsRed labeled with superparamagnetic iron oxide (SPIO) nanoparticles. DiD (lipophilic fluorescent dye)-labeled EPCs were injected into normal mice and mice with liver carcinoma. The EPCs loaded with CD-ES were infused into the mice through caudal veins and tumor volumes were measured. The tumor volumes in the EPC + SPIO + CD/5-Fc + ES group were found to be smaller as a result and grew more slowly than those from the EPC + SPIO + LV (lentivirus, empty vector control) group. Survival times were also measured after infusion of the cells into the mice. The median survival time was found to be longer in the EPC + SPIO + CD/5-Fc + ES group than in the others. In conclusion, the EPCs transfected with CD-ES suppressed the liver carcinoma cells in vitro, migrated primarily to the carcinoma, inhibited tumor growth, and also extended the median survival time for the mice with liver carcinoma.
Rong
R
Chen
Hui
H
Yu
Yan-Li
YL
An
Hua-Jun
HJ
Chen
ZhenYu
Z
Jia
Gao-Jun
GJ
Teng
D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D006528:Carcinoma, Hepatocellular / Q000473:pathology / Q000628:therapy*; D002465:Cell Movement; D043525:Cytosine Deaminase / Q000627:therapeutic use*; D043169:Endostatins / Q000627:therapeutic use*; D066026:Endothelial Progenitor Cells / Q000502:physiology / Q000637:transplantation*; D008114:Liver Neoplasms, Experimental / Q000473:pathology / Q000628:therapy*; D058185:Magnetite Nanoparticles; D051379:Mice; D008810:Mice, Inbred C57BL
D000970:Antineoplastic Agents; D043169:Endostatins; D058185:Magnetite Nanoparticles; D043525:Cytosine Deaminase