Deferoxamine improves antioxidative protection in the brain of neonatal rats: The role of anoxia and body temperature.
After hypoxic-ischemic insult iron deposited in the brain catalyzes formation of reactive oxygen species. Newborn rats, showing reduced physiological body temperature and their hyperthermic counterparts injected with deferoxamine (DF), a chelator of iron, are protected both against iron-mediated neurotoxicity and against depletion of low-molecular antioxidants after perinatal asphyxia. Therefore, we decided to study the effects of DF on activity of antioxidant enzymes (superoxide dismutase-SOD, glutathione peroxidase-GPx and catalase-CAT) in the brain of rats exposed neonatally to a critical anoxia at body temperatures elevated to 39°C. Perinatal anoxia under hyperthermic conditions intensified oxidative stress and depleted the pool of antioxidant enzymes. Both the depletion of antioxidants and lipid peroxidation were prevented by post-anoxic DF injection. The present paper evidenced that deferoxamine may act by recovering of SOD, GPx and CAT activity to reduce anoxia-induced oxidative stress.
N. Copernicus University, Department of Animal Physiology, Faculty of Biology and Environmental Protection, Lwowska 1 St., 87-100 Toruń, Poland. Electronic address: kletkiewicz@doktorant.umk.pl.
Hanna
H
Kletkiewicz
N. Copernicus University, Department of Animal Physiology, Faculty of Biology and Environmental Protection, Lwowska 1 St., 87-100 Toruń, Poland. Electronic address: noann@umk.pl.
Anna
A
Nowakowska
N. Copernicus University, Department of Animal Physiology, Faculty of Biology and Environmental Protection, Lwowska 1 St., 87-100 Toruń, Poland. Electronic address: siejka@umk.pl.
Agnieszka
A
Siejka
N. Copernicus University, The Chair of Medical Biology, Collegium Medicum, Karłowicza 24 St., 85-092 Bydgoszcz, Poland. Electronic address: celestyna_mila@cm.umk.pl.
Celestyna
C
Mila-Kierzenkowska
N. Copernicus University, The Chair of Medical Biology, Collegium Medicum, Karłowicza 24 St., 85-092 Bydgoszcz, Poland. Electronic address: al1103@cm.umk.pl.
Alina
A
Woźniak
N. Copernicus University, Department of Animal Physiology, Faculty of Biology and Environmental Protection, Lwowska 1 St., 87-100 Toruń, Poland. Electronic address: caputa@umk.pl.
Michał
M
Caputa
N. Copernicus University, Department of Animal Physiology, Faculty of Biology and Environmental Protection, Lwowska 1 St., 87-100 Toruń, Poland. Electronic address: rogal@umk.pl.
Justyna
J
Rogalska
D000818:Animals; D000831:Animals, Newborn; D000975:Antioxidants / Q000378:metabolism*; D001831:Body Temperature; D001921:Brain / Q000201:enzymology*; D002374:Catalase / Q000378:metabolism; D003676:Deferoxamine / Q000008:administration & dosage*; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D000860:Hypoxia / Q000201:enzymology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017262:Siderophores / Q000008:administration & dosage*; D000072105:Superoxide Dismutase-1 / Q000378:metabolism
D000975:Antioxidants; D017262:Siderophores; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; C000606292:Sod1 protein, rat; D000072105:Superoxide Dismutase-1; D003676:Deferoxamine
Antioxidant enzymes; Deferoxamine; Hyperthermia; Neonatal anoxia