Early Steps of Dauricine Biosynthesis in Cultured Roots of Menispermum dauricum.
Cultured roots of Menispermum dauricum, a rich source of the bisbenzylisoquinoline alkaloid dauricine (1), were fed with l-[U-(14)C] tyrosine, L-[3-(13)C] tyrosine, and [2-(13)C] tvramine independently, and the incorporation of possible early precursors into 1 was studied. The results demonstrated that 1 was composed of four molecules of tyrosine, and that tyramine was specifically incorporated into the isoquinoline portions of 1. The unusual chlorine-containing alkaloid acutumine (3), into which (14)C-labeled tyrosine was also incorporated, was identified as one of the main constituents in the alkaloid fraction from the roots.
a Arid Land Research Center, Tottori University , 1390 Hamasaka, Tottori, Tottori 680 , Japan.
Y
Y
Sugimoto
a Arid Land Research Center, Tottori University , 1390 Hamasaka, Tottori, Tottori 680 , Japan.
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Uchida
a Arid Land Research Center, Tottori University , 1390 Hamasaka, Tottori, Tottori 680 , Japan.
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Inanaga
a Arid Land Research Center, Tottori University , 1390 Hamasaka, Tottori, Tottori 680 , Japan.|b Department of Agriculture , Tottori University , 4-101 Koyama-cho, Minami, Tottori, Tottori 680 , Japan.
Y
Y
Kimura
a Arid Land Research Center, Tottori University , 1390 Hamasaka, Tottori, Tottori 680 , Japan.|c Nitto Technical Information Center Co., Ltd. , 1-1-2 Shimohozumi, Iharaki, Osaka 567 , Japan.
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Hashimoto
a Arid Land Research Center, Tottori University , 1390 Hamasaka, Tottori, Tottori 680 , Japan.|d Nara Institute of Science and Technology , 8916-5 Takayama-cho, Ikoma, Nara 630-01 , Japan.
A
A
Isogai
Menispermum dauricum; biosynthesis; bisbenzylisoquinoline; dauricine; root culture