Omega-3 Fatty Acids: Possible Neuroprotective Mechanisms in the Model of Global Ischemia in Rats.
Background. Omega-3 (ω3) administration was shown to protect against hypoxic-ischemic injury. The objectives were to study the neuroprotective effects of ω3, in a model of global ischemia. Methods. Male Wistar rats were subjected to carotid occlusion (30 min), followed by reperfusion. The groups were SO, untreated ischemic and ischemic treated rats with ω3 (5 and 10 mg/kg, 7 days). The SO and untreated ischemic animals were orally treated with 1% cremophor and, 1 h after the last administration, they were behaviorally tested and euthanized for neurochemical (DA, DOPAC, and NE determinations), histological (Fluoro jade staining), and immunohistochemical (TNF-alpha, COX-2 and iNOS) evaluations. The data were analyzed by ANOVA and Newman-Keuls as the post hoc test. Results. Ischemia increased the locomotor activity and rearing behavior that were partly reversed by ω3. Ischemia decreased striatal DA and DOPAC contents and increased NE contents, effects reversed by ω3. This drug protected hippocampal neuron degeneration, as observed by Fluoro-Jade staining, and the increased immunostainings for TNF-alpha, COX-2, and iNOS were partly or totally blocked by ω3. Conclusion. This study showed a neuroprotective effect of ω3, in great part due to its anti-inflammatory properties, stimulating translational studies focusing on its use in clinic for stroke managing.
Faculty of Medicine, Estácio of Juazeiro do Norte (FMJ), Rua Tenente Raimundo Rocha 515, 63040-360 Juazeiro do Norte, CE, Brazil.
Maria Elizabeth Pereira
0000-0003-4494-6116
ME
Nobre
Faculty of Medicine, Estácio of Juazeiro do Norte (FMJ), Rua Tenente Raimundo Rocha 515, 63040-360 Juazeiro do Norte, CE, Brazil.
Alyne Oliveira
AO
Correia
Faculty of Medicine, Estácio of Juazeiro do Norte (FMJ), Rua Tenente Raimundo Rocha 515, 63040-360 Juazeiro do Norte, CE, Brazil.
Francisco Nilson Maciel
FN
Mendonça
Faculty of Medicine, Estácio of Juazeiro do Norte (FMJ), Rua Tenente Raimundo Rocha 515, 63040-360 Juazeiro do Norte, CE, Brazil.
Luiz Ricardo Araújo
LR
Uchoa
Faculty of Medicine, Estácio of Juazeiro do Norte (FMJ), Rua Tenente Raimundo Rocha 515, 63040-360 Juazeiro do Norte, CE, Brazil.
Jessica Tamara Nunes
JT
Vasconcelos
Faculty of Medicine, Estácio of Juazeiro do Norte (FMJ), Rua Tenente Raimundo Rocha 515, 63040-360 Juazeiro do Norte, CE, Brazil.
Carlos Ney Alencar
CN
de Araújo
Federal University of Ceará (UFC), Rua Coronel Nunes de Melo 1127, 60430-270 Fortaleza, CE, Brazil.
Gerly Anne de Castro
0000-0002-8214-4379
GA
Brito
Federal University of Ceará (UFC), Rua Coronel Nunes de Melo 1127, 60430-270 Fortaleza, CE, Brazil.
Rafaelly Maria Pinheiro
RM
Siqueira
Federal University of Ceará (UFC), Rua Coronel Nunes de Melo 1127, 60430-270 Fortaleza, CE, Brazil.
Gilberto Dos Santos
0000-0001-6717-3772
Gdos S
Cerqueira
Federal University of Ceará (UFC), Rua Coronel Nunes de Melo 1127, 60430-270 Fortaleza, CE, Brazil.
Kelly Rose Tavares
KR
Neves
Federal University of São Paulo (UNIFESP), Rua Pedro de Toledo 669, 04039-032 São Paulo, SP, Brazil.
Ricardo Mário
RM
Arida
Faculty of Medicine, Estácio of Juazeiro do Norte (FMJ), Rua Tenente Raimundo Rocha 515, 63040-360 Juazeiro do Norte, CE, Brazil; Federal University of Ceará (UFC), Rua Coronel Nunes de Melo 1127, 60430-270 Fortaleza, CE, Brazil.
Glauce Socorro de Barros
0000-0002-9996-9537
GS
Viana