Sanguisorba officinalis L. Extracts Exert Antiobesity Effects in 3T3-L1 Adipocytes and C57BL/6J Mice Fed High-Fat Diets.
The purpose of this study was to investigate the antiobesity effect of Sanguisorba officinalis L. (SOL) in 3T3-L1 adipocytes and obese C57BL/6J mice. SOL was extracted with water and 30%, 50%, 70%, and 100% ethanol (EtOH). 3T3-L1 adipocytes were treated with SOL extracts (100 μg/mL) during the differentiation period. Triglyceride (TG) accumulation was determined by Oil Red O staining, and the expression of adipocyte-specific proteins was measured by Western blot analysis. C57BL/6J mice were fed a high-fat diet to induce obesity and were orally administered SOL 50% ethanol extract (50, 100, and 200 mg/kg) for 8 weeks. Among the SOL extracts, the 50% EtOH extract considerably inhibited TG accumulation through the downregulation of PPARγ, C/EBPα, and SREBP-1c in 3T3-L1 adipocytes. In addition, the 50% ethanol extract reduced body weight and adipose tissue weight and improved serum lipid profiles through downregulation of PPARγ, C/EBPα, FABP4, and ACC and upregulation of adiponectin and CPT-1 in obese C57BL/6J mice fed a high-fat diet. These results suggested that the SOL 50% EtOH extract may have an antiobesity effect through the regulation of transcription factors related to adipogenesis, lipogenesis, and lipolysis.
1 Department of Food Science and Nutrition, Hallym University , Gangwon, Korea.
Da-Woon
DW
Jung
2 Department of Food Science and Biotechnology, Kangwon National University , Gangwon, Korea.
Ok-Hwan
OH
Lee
1 Department of Food Science and Nutrition, Hallym University , Gangwon, Korea.
Il-Jun
IJ
Kang
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism; D050156:Adipogenesis; D052242:Adiponectin / Q000097:blood; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology* / Q000627:therapeutic use; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000097:blood; D059305:Diet, High-Fat; D050356:Lipid Metabolism / Q000187:drug effects*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D009765:Obesity / Q000097:blood* / Q000209:etiology; D047495:PPAR gamma / Q000097:blood; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D031993:Sanguisorba; D051780:Sterol Regulatory Element Binding Protein 1 / Q000097:blood; D014157:Transcription Factors / Q000097:blood*; D014280:Triglycerides / Q000097:blood; D015431:Weight Loss / Q000187:drug effects
D052242:Adiponectin; D019440:Anti-Obesity Agents; D022763:CCAAT-Enhancer-Binding Protein-alpha; D047495:PPAR gamma; D010936:Plant Extracts; D051780:Sterol Regulatory Element Binding Protein 1; D014157:Transcription Factors; D014280:Triglycerides
3T3-L1 adipocytes; Sanguisorba officinalis L.; adipogenesis; antiobesity; lipolysis