Isolation and characterization of a distinct duck-origin goose parvovirus causing an outbreak of duckling short beak and dwarfism syndrome in China.
Many mule duck and Cherry Valley duck flocks in different duck-producing regions of China have shown signs of an apparently new disease designated "short beak and dwarfism syndrome" (SBDS) since 2015. The disease is characterized by dyspraxia, weight loss, a protruding tongue, and high morbidity and low mortality rates. In order to characterize the etiological agent, a virus designated SBDSV M15 was isolated from allantoic fluid of dead embryos following serial passage in duck embryos. This virus causes a cytopathic effect in duck embryo fibroblast (DEF) cells. Using monoclonal antibody diagnostic assays, the SBDSV M15 isolate was positive for the antigen of goose parvovirus but not Muscovy duck parvovirus. A 348-bp (2604-2951) VP1gene fragment was amplified, and its sequence indicated that the virus was most closely related to a Hungarian GPV strain that was also isolated from mule ducks with SBDS disease. A similar disease was reproduced by inoculating birds with SBDSV M15. Together, these data indicate that SBDSV M15 is a GPV-related parvovirus causing SBDS disease and that it is divergent from classical GPV isolates.
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences, No. 247 Wusi Road, Fuzhou, 350003, China.|College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
Shilong
S
Chen
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences, No. 247 Wusi Road, Fuzhou, 350003, China.
Shao
S
Wang
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences, No. 247 Wusi Road, Fuzhou, 350003, China.
Xiaoxia
X
Cheng
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences, No. 247 Wusi Road, Fuzhou, 350003, China.
Shifeng
S
Xiao
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences, No. 247 Wusi Road, Fuzhou, 350003, China.
Xiaoli
X
Zhu
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences, No. 247 Wusi Road, Fuzhou, 350003, China.
Fengqiang
F
Lin
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences, No. 247 Wusi Road, Fuzhou, 350003, China.
Nanyang
N
Wu
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences, No. 247 Wusi Road, Fuzhou, 350003, China.
Jinxiang
J
Wang
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences, No. 247 Wusi Road, Fuzhou, 350003, China.
Meiqing
M
Huang
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences, No. 247 Wusi Road, Fuzhou, 350003, China.
Min
M
Zheng
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences, No. 247 Wusi Road, Fuzhou, 350003, China. chensy58@163.com.
Shaoying
S
Chen
Institute of Biotechnology, Fujian Academy of Agricultural Sciences, Fuzhou, 350003, China. yufusong58@163.com.
Fusong
F
Yu
D000818:Animals; D001502:Beak / Q000473:pathology*; D002681:China / Q000453:epidemiology; D004372:Ducks; D004392:Dwarfism / Q000662:veterinary* / Q000821:virology; D018445:Infectious Disease Transmission, Vertical; D007841:Latex Fixation Tests; D033401:Microscopy, Acoustic; D017992:Parvovirus / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity / Q000648:ultrastructure; D010802:Phylogeny; D011201:Poultry Diseases / Q000453:epidemiology / Q000473:pathology / Q000821:virology*; D012698:Serologic Tests / Q000662:veterinary