Molecular characterization and antibiotic resistance of group G streptococci in Israel: comparison of invasive, non-invasive and carriage isolates.
Beta-hemolytic group G streptococci (GGS) are increasingly recognized as a source of substantial morbidity, causing mild to severe sporadic infections as well as outbreaks. The purpose of this study was to determine the genetic diversity and antibiotic resistance of GGS in Israel in order to aid in prevention and control. A total of 325 GGS isolates were collected in Israel between 2007 and 2011 from three determined settings: (1) carriage (n = 60), an observational longitudinal carriage study in the IF, (2) non-invasive (n = 166), clinical sporadic and epidemic non-invasive cases in the IDF, and (3) invasive (n = 99) cases of bacteremia collected during this period in Israel from a similar age group, at the national Streptococcal Reference Center. All isolates were characterized genetically and by their antibiotic-resistance profile. emm typing revealed 35 distinct types and subtypes among 228 S. dysgalactiae subsp. equisimilis (SDSE) isolates, with high genetic diversity. An additional 97 GGS were identified as Streptococcus anginosus (SAG). The proportion of SDSE was higher in the invasive (100 %) and non-invasive (63.8 %) isolates compared to the carriage ones (38.3 %). Clindamycin, erythromycin, azithromycin and tetracycline resistance was detected in 6.6 %, 8.6 %, 9.7 % and 37.6 % of isolates, respectively. Overall, the most resistant isolates were in the invasive group and the fewest were in the SAG group. Considerable genetic diversity and common antibiotic resistance were revealed among GGS strains which differed according to the epidemiologic settings. Further clinical, epidemiological and basic research of GGS as a pathogen is warranted.
Israel Defense Forces Medical Corps, Tel HaShomer, Israel. halperintami@gmail.com.
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Halperin
Hebrew University-Hadassah Braun School of Public Health and Community Medicine, Jerusalem, Israel.
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Levine
Government Central Laboratories, Ministry of Health, Jerusalem, Israel.
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Korenman
Israel Defense Forces Medical Corps, Tel HaShomer, Israel.
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Burstein
Israel Defense Forces Medical Corps, Tel HaShomer, Israel.
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Amber
Israel Defense Forces Medical Corps, Tel HaShomer, Israel.
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Sela
Government Central Laboratories, Ministry of Health, Jerusalem, Israel.
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Valinsky
D000293:Adolescent; D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D016470:Bacteremia / Q000382:microbiology*; D002353:Carrier State / Q000382:microbiology*; D024881:Drug Resistance, Bacterial; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007557:Israel; D008137:Longitudinal Studies; D008297:Male; D008875:Middle Aged; D013290:Streptococcal Infections / Q000382:microbiology*; D013291:Streptococcus / Q000145:classification* / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D055815:Young Adult
D000900:Anti-Bacterial Agents