Incorporation and visualization of azido-functionalized N-oleoyl serinol in Jurkat cells, mouse brain astrocytes, 3T3 fibroblasts and human brain microvascular endothelial cells.
The synthesis and biological evaluation of azido-N-oleoyl serinol is reported. It mimicks biofunctional lipid ceramides and has shown to be capable of click reactions for cell membrane imaging in Jurkat and human brain microvascular endothelial cells.
Institute for Organic Chemistry, Julius-Maximilians University Würzburg, Am Hubland C1, Würzburg, Germany.
T
T
Walter
Institute of Virology and Immunobiology, Versbacher Str. 7, Wuerzburg Germany.
L
L
Collenburg
Institute of Nutritional Science, University of Potsdam, Arthur-Scheunert Allee 114-116, Nuthetal, Potsdam, Germany.
L
L
Japtok
Institute of Nutritional Science, University of Potsdam, Arthur-Scheunert Allee 114-116, Nuthetal, Potsdam, Germany.
B
B
Kleuser
Institute of Virology and Immunobiology, Versbacher Str. 7, Wuerzburg Germany.
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S
Schneider-Schaulies
Institute of Virology and Immunobiology, Versbacher Str. 7, Wuerzburg Germany.
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N
Müller
Institute of hygiene and microbiology, Josef-Schneider-Straße 2 / E1, Würzburg.
J
J
Becam
Institute of hygiene and microbiology, Josef-Schneider-Straße 2 / E1, Würzburg.
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A
Schubert-Unkmeir
Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, Augusta University, 1120 15 Street, Augusta, GA 30912 U.S.A.
J N
JN
Kong
Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, Augusta University, 1120 15 Street, Augusta, GA 30912 U.S.A.
E
E
Bieberich
Institute for Organic Chemistry, Julius-Maximilians University Würzburg, Am Hubland C1, Würzburg, Germany.
J
J
Seibel
D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D001386:Azides / Q000737:chemistry*; D001921:Brain / Q000098:blood supply / Q000166:cytology; D042783:Endothelial Cells / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells; D051379:Mice; D055806:Microvessels / Q000166:cytology*; D057054:Molecular Imaging / Q000379:methods*; D011412:Propanolamines / Q000737:chemistry* / Q000378:metabolism*; D011409:Propylene Glycols / Q000737:chemistry* / Q000378:metabolism*
D001386:Azides; D011412:Propanolamines; D011409:Propylene Glycols; C072422:serinol