[Effect of polydatin on miR-214 expression and liver function in ApoE-/- mice].
OBJECTIVE
To study the effect of polydatin on the expression level of miR-214 and liver function in atherosclerotic mice.


METHODS
Forty male ApoE(-/-) mice were randomly allocated into 4 groups (n=10), namely the model group, low- and high-dose polydatin groups, and simvastin group, with 10 male C57BL/6J mice serving as the normal control group. Mouse models of atherosclerosis were established by feeding the ApoE(-/-) mice with a high-fat diet. After 12 weeks of treatment, blood levels of glucose, lipids, AST, and ALT and the contents of T-SOD and MDA in the liver tissue were detected. The pathologies of the liver were examined with HE staining, and miR-214 expression in the liver was detected using quantitative real-time PCR.


RESULTS
Compared with the normal control mice, the mice in the model group showed significantly increased blood glucose, serum TC, TG, LDL-C, ALT, and AST levels, and MDA contents in the liver (P<0.01), with significantly decreased serum HDL-C level and SOD and miR-214 levels in liver (P<0.01). Polydatin treatment significantly ameliorated such changes in blood glucose, serum ALT, AST, TC, TG, LDL-C, and HDL-C levels, and MDA, SOD, and miR-214 contents in liver tissue (P<0.05).


CONCLUSIONS
s Polydatin can reduce blood glucose and lipid levels and protect the liver function in atherosclerotic mice possibly by up-regulating the expression of miR-214 and T-SOD and down-regulating MDA in the liver.
School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China. E-mail: wendyzhou515@126.com.
Feng-Hua
FH
Zhou
Zi-Yun
ZY
Wen
Ze-Huai
ZH
He
Mei
M
Li
Qiong-Li
QL
Yin
Cheng-Gang
CG
Shi
Cai-Lian
CL
Cheng
D000818:Animals; D001057:Apolipoproteins E / Q000235:genetics; D050197:Atherosclerosis / Q000188:drug therapy*; D001786:Blood Glucose / Q000032:analysis; D059305:Diet, High-Fat; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D005960:Glucosides / Q000494:pharmacology*; D008055:Lipids / Q000097:blood; D008099:Liver / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D035683:MicroRNAs / Q000378:metabolism*; D013267:Stilbenes / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
D001057:Apolipoproteins E; D001786:Blood Glucose; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D008055:Lipids; D035683:MicroRNAs; C534781:Mirn214 microRNA, mouse; D013267:Stilbenes; D008315:Malondialdehyde; D013482:Superoxide Dismutase; C058229:polydatin