Antispasmodic Effects and Action Mechanism of Essential Oil of Chrysactinia mexicana A. Gray on Rabbit Ileum.
The Chrysactinia mexicana A. Gray (C. mexicana) plant is used in folk medicine to treat fever and rheumatism; it is used as a diuretic, antispasmodic; and it is used for its aphrodisiac properties. This study investigates the effects of the essential oil of C. mexicana (EOCM) on the contractility of rabbit ileum and the mechanisms of action involved. Muscle contractility studies in vitro in an organ bath to evaluate the response to EOCM were performed in the rabbit ileum. EOCM (1-100 µg·mL(-1)) reduced the amplitude and area under the curve of spontaneous contractions of the ileum. The contractions induced by carbachol 1 µM, potassium chloride (KCl) 60 mM or Bay K8644 1 µM were reduced by EOCM (30 µg·mL(-1)). Apamin 1 µM and charybdotoxin 0.01 µM decreased the inhibition induced by EOCM. The d-cAMP 1 µM decreased the inhibition induced by EOCM. l-NNA 10 µM, Rp-8-Br-PET-cGMPS 1 µM, d,l-propargylglycine 2 mM, or aminooxyacetic acid hemihydrochloride 2 mM did not modify the EOCM effect. In conclusion, EOCM induces an antispasmodic effect and could be used in the treatment of intestinal spasms or diarrhea processes. This effect would be mediated by Ca(2+), Ca(2+)-activated K⁺ channels and cAMP.
Departamento de Farmacolog&#237;a y Fisiolog&#237;a (Fisiolog&#237;a), Facultad de Veterinaria, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain. menzd_che@yahoo.com.mx.|Direcci&#243;n General de Servicios Educativos Iztapalapa, Av. Rojo G&#243;mez No. 1149, Col. Barrio San Pedro, Del Iztapalapa 09300 M&#233;xico, DF, Mexico. menzd_che@yahoo.com.mx.
Daniel
D
Zavala-Mendoza
Departamento de Farmacolog&#237;a y Fisiolog&#237;a (Fisiolog&#237;a), Facultad de Veterinaria, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain. lgralo@unizar.es.|Instituto de Investigaci&#243;n Sanitaria de Arag&#243;n (IIS-Arag&#243;n), 50013 Zaragoza, Spain. lgralo@unizar.es.|Instituto Agroalimentario de Arag&#243;n (IA2), 50013 Zaragoza, Spain. lgralo@unizar.es.
Laura
L
Grasa
Departamento de Sistemas Biol&#243;gicos, Universidad Aut&#243;noma Metropolitana-Xochimilco, Calzada del Hueso 1100, Colonia Villa Quietud, Coyoac&#225;n, 09340 Mexico, DF, Mexico. migzavalasan@gmail.com.
Miguel Ángel
MÁ
Zavala-Sánchez
Departamento de Sistemas Biol&#243;gicos, Universidad Aut&#243;noma Metropolitana-Xochimilco, Calzada del Hueso 1100, Colonia Villa Quietud, Coyoac&#225;n, 09340 Mexico, DF, Mexico. msperez@correo.xoc.uam.mx.
Salud
S
Pérez-Gutiérrez
Departamento de Farmacolog&#237;a y Fisiolog&#237;a (Fisiolog&#237;a), Facultad de Veterinaria, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain. divinamurillo@hotmail.com.|Instituto de Investigaci&#243;n Sanitaria de Arag&#243;n (IIS-Arag&#243;n), 50013 Zaragoza, Spain. divinamurillo@hotmail.com.
María Divina
MD
Murillo
D000818:Animals; D001030:Apamin / Q000008:administration & dosage; D019659:Asteraceae / Q000737:chemistry; D002118:Calcium / Q000378:metabolism; D006801:Humans; D007082:Ileum / Q000187:drug effects* / Q000473:pathology; D009119:Muscle Contraction / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects; D009822:Oils, Volatile / Q000008:administration & dosage* / Q000737:chemistry; D010276:Parasympatholytics / Q000008:administration & dosage* / Q000737:chemistry; D010938:Plant Oils / Q000008:administration & dosage* / Q000737:chemistry; D011189:Potassium Chloride / Q000737:chemistry; D011817:Rabbits
D009822:Oils, Volatile; D010276:Parasympatholytics; D010938:Plant Oils; D001030:Apamin; D011189:Potassium Chloride; D002118:Calcium
Chrysactinia mexicana; antispasmodic effect; essential oil; ileum; intestinal motility; rabbit