Molecular cloning and analysis of a receptor-like promoter of Gbvdr3 gene in sea island cotton.
Verticillium wilt caused by soil borne fungus Verticillium dahliae could significantly reduce cotton yield. The Ve1 homologous gene Gbvdr3 is resistant to Verticillium wilt. In order to understand of the function of the promoter Gbvdr3 in Gossypium barbadense, the promoter region of the receptor-like gene Gbvdr3 was obtained by genome walking, and the cis-element in the promoter was identified using the PLACE software in this study. The sequence analysis showed that the promoter contained elements related to stress resistance and light regulation. The cloned promoter was fused to the GUS reporter gene and transformed into Arabidopsis. GUS expression was specifically detected in roots, flowers, and seeds, suggesting that the expression of Gbvdr3 is tissue-specific. Separation and characterization analysis of the promoter of Gbvdr3 provides a platform for further research and application of this gene. Thorough understanding of the function of the Gbvdr3 promoter is important for better understanding of Gbvdr3 function. These results indicated that the promoter of Gbvdr3 was a tissue-specific promoter.
Nanjing Xiaozhuang University, Nanjing, China.|College of Life Sciences, Anhui Agricultural University, Hefei, China.
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BJ
Zhang
College of Life Sciences, Anhui Agricultural University, Hefei, China.
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HP
Zhang
Nanjing Xiaozhuang University, Nanjing, China.
Q-Z
QZ
Chen
Nanjing Xiaozhuang University, Nanjing, China.
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N
Tang
Nanjing Xiaozhuang University, Nanjing, China.
L-K
LK
Wang
College of Life Sciences, Anhui Agricultural University, Hefei, China.
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RF
Wang
Institute of Agro-biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
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BL
Zhang
D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development; D003001:Cloning, Molecular; D060467:Disease Resistance / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D003368:Gossypium / Q000235:genetics* / Q000254:growth & development / Q000821:virology; D010935:Plant Diseases / Q000235:genetics* / Q000821:virology; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D018517:Plant Roots; D030821:Plants, Genetically Modified / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D012988:Soil Microbiology; D013784:Textiles; D020172:Verticillium / Q000235:genetics / Q000472:pathogenicity
D010940:Plant Proteins