pDGO100, a type 1 IncC plasmid from 1981 carrying ARI-A and a Tn1696-like transposon in a novel integrating element.
Most A/C plasmids sequenced to date were recovered in the last two decades. To gain insight into the evolution of this group, the IncC plasmid pDGO100, found in a multiply antibiotic-resistant Escherichia coli strain isolated in 1981, was sequenced. pDGO100 belongs to the type 1 lineage and carries an ARI-A antibiotic resistance island but not an ARI-B island. The A/C2 backbone of pDGO100 has a deletion in the rhs1 gene previously found in pRMH760 and differs by only six single base pair substitutions from pRMH760, recovered at the same hospital 16years later. This confirms that the separation of type 1 and type 2 IncC plasmids is long standing. The ARI-A islands are also closely related, but pRMH760 contains Tn4352B in tniA of Tn402, while in pDGO100, Tn4352 has inserted into merA of pDUmer. pDGO100 also carries an additional 46kb insertion that includes a Tn1696-like transposon with the dfrB3 gene cassette. This insertion was identified as a novel integrating element, with an int gene at one end, and also includes the fec iron uptake operon that has been acquired from the E. coli chromosome. Related integrating elements carrying the same int gene were found in A/C2, IncHI1, and IncHI2 plasmids, and in the chromosomes of Enterobacter cloacae, Klebsiella oxytoca, and Cronobacter sakazakii isolates. In the Enterobacteriaceae chromosomes, these integrating elements appear to target a gene encoding a radical SAM superfamily protein. In the A/C2, IncHI1, and IncHI2 plasmids, genes encoding a phosphoadenosine phosphosulfate reductase were interrupted. The extremities of the integrating element are highly conserved, whilst the internal gene content varies. The detection of integrative elements in plasmids demonstrates an increased range of locations into which this type of mobile element can integrate and insertion in plasmids is likely to assist their spread.
School of Molecular Bioscience, The University of Sydney, Sydney, New South Wales, Australia. Electronic address: christopher.harmer@sydney.edu.au.
Christopher J
CJ
Harmer
Centre for Infectious Diseases and Microbiology, The Westmead Institute for Medical Research, Westmead Hospital, The University of Sydney, Sydney, New South Wales, Australia.
Sally R
SR
Partridge
School of Molecular Bioscience, The University of Sydney, Sydney, New South Wales, Australia.
Ruth M
RM
Hall
D000449:Aldehyde Reductase / Q000235:genetics; D019943:Amino Acid Substitution; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001483:Base Sequence; D044083:Cronobacter sakazakii / Q000235:genetics / Q000302:isolation & purification; D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D016972:Enterobacter cloacae / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics* / Q000302:isolation & purification*; D017353:Gene Deletion; D006801:Humans; D041121:Klebsiella oxytoca / Q000235:genetics / Q000302:isolation & purification; D009097:Multienzyme Complexes / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011956:Receptors, Cell Surface / Q000235:genetics; D017422:Sequence Analysis, DNA; D013430:Sulfate Adenylyltransferase / Q000235:genetics
D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D009097:Multienzyme Complexes; D011956:Receptors, Cell Surface; D000449:Aldehyde Reductase; C022322:PAPS synthetase; D013430:Sulfate Adenylyltransferase
A/C(2); Antibiotic resistance; Evolution in situ; IncC; Integrating element