Effective Separation and Simultaneous Determination of Corynoxeine and Its Metabolites in Rats by High-Performance Liquid Chromatography with Tandem Mass Spectrometry and Application to Pharmacokinetics and In Vivo Distribution in Main Organs.
An effective separation and simultaneous determination of corynoxeine and its metabolites using high-performance liquid chromatography with tandem mass spectrometry was developed and validated. The method was applied to pharmacokinetics and in vivo distribution investigations in rats after oral (0.105 mmol kg(-1)) and intravenous (0.0105 mmol kg(-1)) doses of corynoxeine. Its brain uptake index was of 3.08 × 10(-11) mol g(-1) at 3 h and 3.75 × 10(-11) mol g(-1) at 74 min after oral and intravenous doses, respectively.
School of Pharmaceutical Sciences and Yunnan Provincial Key Laboratory of Pharmacology for Natural Products, Kunming Medical University.
Wei
W
Wang
Shaozhong
S
Luo
Yaping
Y
Chen
Bo
B
Li
Masao
M
Hattori
D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D026121:Indole Alkaloids / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000493:pharmacokinetics*; D008297:Male; D051381:Rats; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013997:Time Factors; D014018:Tissue Distribution
D026121:Indole Alkaloids; C054799:corynoxeine