Light-Responsive and pH-Responsive DNA Microcapsules for Controlled Release of Loads.
A method to assemble light-responsive or pH-responsive microcapsules loaded with different loads (tetramethylrhodamine-modified dextran, TMR-D; microperoxidase-11, MP-11; CdSe/ZnS quantum dots; or doxorubicin-modified dextran, DOX-D) is described. The method is based on the layer-by-layer deposition of sequence-specific nucleic acids on poly(allylamine hydrochloride)-functionalized CaCO3 core microparticles, loaded with the different loads, that after the dissolution of the core particles with EDTA yields the stimuli-responsive microcapsules that include the respective loads. The light-responsive microcapsules are composed of photocleavable o-nitrobenzyl-phosphate-modified DNA shells, and the pH-responsive microcapsules are made of a cytosine-rich layer cross-linked by nucleic acid bridges. Irradiating the o-nitrobenzyl phosphate-functionalized microcapsules, λ = 365 nm, or subjecting the pH-responsive microcapsules to pH = 5.0, results in the cleavage of the microcapsule shells and the release of the loads. Preliminary studies address the cytotoxicity of the DOX-D-loaded microcapsules toward MDA-MB-231 breast cancer cells and normal MCF-10A breast epithelial cells. Selective cytotoxicity of the DOX-D-loaded microcapsules toward cancer cells is demonstrated.
Institute of Chemistry, Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem , Jerusalem 91904, Israel.
Fujian
F
Huang
Institute of Chemistry, Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem , Jerusalem 91904, Israel.
Wei-Ching
WC
Liao
Institute of Life Sciences, The Hebrew University of Jerusalem , Jerusalem 91904, Israel.
Yang Sung
YS
Sohn
Institute of Life Sciences, The Hebrew University of Jerusalem , Jerusalem 91904, Israel.
Rachel
R
Nechushtai
Institute of Chemistry, Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem , Jerusalem 91904, Israel.
Chun-Hua
CH
Lu
Institute of Chemistry, Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem , Jerusalem 91904, Israel.
Itamar
I
Willner
D001692:Biological Transport; D002119:Calcium Carbonate / Q000737:chemistry; D002214:Capsules; D045744:Cell Line, Tumor; D004247:DNA / Q000737:chemistry*; D003692:Delayed-Action Preparations; D004317:Doxorubicin / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism; D065546:Drug Liberation; D004492:Edetic Acid / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008027:Light
D002214:Capsules; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D004317:Doxorubicin; D004247:DNA; D004492:Edetic Acid; D002119:Calcium Carbonate