Synthesis of β-1,4-Linked Galactan Side-Chains of Rhamnogalacturonan I.
The synthesis of linear- and (1→6)-branched β-(1→4)-d-galactans, side-chains of the pectic polysaccharide rhamnogalacturonan I is described. The strategy relies on iterative couplings of n-pentenyl disaccharides followed by a late stage glycosylation of a common hexasaccharide core. Reaction with a covalent linker and immobilization on N-hydroxysuccinimide (NHS)-modified glass surfaces allows the generation of carbohydrate microarrays. The glycan arrays enable the study of protein-carbohydrate interactions in a high-throughput fashion, demonstrated herein with binding studies of mAbs and a CBM.
Center for Nanomedicine and Theranostics, Department of Chemistry, Technical University of Denmark, Kemitorvet, Building 207, 2800 Kgs., Lyngby, Denmark.
Mathias C F
MC
Andersen
Department of Plant and Environmental Sciences, Thorvaldsensvej 40, Frederiksberg C, 1871, Denmark.
Stjepan K
SK
Kračun
Department of Plant and Environmental Sciences, Thorvaldsensvej 40, Frederiksberg C, 1871, Denmark.
Maja G
MG
Rydahl
Department of Plant and Environmental Sciences, Thorvaldsensvej 40, Frederiksberg C, 1871, Denmark.|School of Agriculture, Food and Rural Development, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK.
William G T
WG
Willats
Center for Nanomedicine and Theranostics, Department of Chemistry, Technical University of Denmark, Kemitorvet, Building 207, 2800 Kgs., Lyngby, Denmark. mhc@kemi.dtu.dk.
Mads H
http://orcid.org/0000-0001-9649-1729
MH
Clausen
D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology; D005685:Galactans / Q000737:chemistry* / Q000378:metabolism; D010368:Pectins / Q000138:chemical synthesis* / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D013388:Succinimides / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D005685:Galactans; D011134:Polysaccharides; D013388:Succinimides; C042491:rhamnogalacturonan I; D010368:Pectins; C001426:N-hydroxysuccinimide
carbohydrates; glycosylation; oligosaccharides; plant cell walls; rhamnogalacturonan