Agrimol B suppresses adipogenesis through modulation of SIRT1-PPAR gamma signal pathway.
Studies of human genetics have implicated the role of SIRT1 in regulating obesity, insulin resistance, and longevity. These researches motivated the identification of novel SIRT1 activators. The current study aimed to investigate the potential efficacy of agrimol B, a polyphenol derived from Agrimonia pilosa Ledeb., on mediating SIRT1 activity and fat metabolism. Results showed that agrimol B significantly induced cytoplasm-to-nucleus shuttle of SIRT1. Furthermore, we confirmed that agrimol B dramatically inhibited 3T3-L1 adipocyte differentiation by reducing PPARγ, C/EBPα, FAS, UCP-1, and apoE expression. Consequently, adipogenesis was blocked by treatment of agrimol B at the early stage of differentiation in a dose-dependent manner, the IC50 value was determined as 3.35 ± 0.32 μM. Taken together, our data suggest a therapeutic potential of agrimol B on alleviating obesity, through modulation of SIRT1-PPARγ signal pathway.
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
Shifeng
S
Wang
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
Qiao
Q
Zhang
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
Yuxin
Y
Zhang
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
Cheng
C
Shen
Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China.
Zhen
Z
Wang
HD Biosciences Co., Ltd, Shanghai 201201, China.
Qinghua
Q
Wu
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
Yanling
Y
Zhang
Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China. Electronic address: lishiyou@big.ac.cn.
Shiyou
S
Li
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. Electronic address: yjqiao@bucm.edu.cn.
Yanjiang
Y
Qiao
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D002074:Butanones / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D051379:Mice; D047495:PPAR gamma / Q000378:metabolism*; D010636:Phenols / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects*; D056564:Sirtuin 1 / Q000378:metabolism*
D002074:Butanones; D047495:PPAR gamma; D010636:Phenols; C062407:agrimol B; C470545:Sirt1 protein, mouse; D056564:Sirtuin 1
Agrimol B; Agrimonia pilosa Ledeb.; Lipid droplet; Obesity; PPARγ; SIRT1