The Effect of Hemiscorpius lepturus (Scorpionida: Hemiscorpiidae) Venom on Leukocytes and the Leukocyte Subgroups in Peripheral Blood of Rat.
BACKGROUND
The aim of this study was to investigate the effect of Hemiscorpius lepturus venom on leukocytes and the leukocyte subgroups in peripheral blood of rat.


METHODS
In this experimental study, sixty N-Mari rats were divided into three groups of 20 rats. Then the rats in each group were divided into four subgroups based on the blood sampling time that was 2, 6, 24 and 48 hours after the venom injection, respectively. The control group did not receive anything, however, the first and the second experimental groups received 0.1 and 0.01mg/kg of venom, subcutaneously. In accordance with a designated four sampling times, the blood sampling was carried out in three groups. After RBC lysis, the leukocytes and leukocyte sub-populations were determined and counted using appropriate hematological standard methods.


RESULTS
The leukocyte and the neutrophil count at two (P<0.05), six (P<0.01) and 24 (P<0.05) hours after the venom injection showed a significant decline compared with the control group, this decrease was significant at the dose of 0.1 mg/kg until 48 hours after the venom injection (P<0.05). The lymphocyte count showed a significant decline throughout the all hours of the experiment, compared with the control group (P<0.05).


CONCLUSIONS
Leukocytes are probably affected by the cytotoxicity effect of the H. lepturus venom in a dose-dependent manner. This could be a wakeup call for the medical staff to perform quick and accurate treatment in the least time possible.
Health Research Institute, Research Center of Thalassemia and Hemoglobinopathy, Department of Immunology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Mehri
M
Ghafourian
Department of Pharmacology, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Neda
N
Ganjalikhanhakemi
Department of Pharmacology and Toxicology, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Ali Asghar
AA
Hemmati
Department of Environmental Health, School of Health and Social Determinants of Health (SDH), Research Center, Kashan University of Medical Sciences, Kashan, Iran.
Rouhullah
R
Dehghani
Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Wesam
W
Kooti
Hemiscorpius lepturus; Leucocyte; Rat; Venom