Effect of feeding lambs with a tanniferous shrub (rockrose) and a vegetable oil blend on fatty acid composition of meat lipids.
The effects of feeding Cistus ladanifer (Cistus) and a blend of soybean and linseed oil (1 : 2 vol/vol) on fatty acid (FA) composition of lamb meat lipids and messenger RNA (mRNA) expression of desaturase enzymes was assessed. In total, 54 male lambs were randomly assigned to 18 pens and to nine diets, resulting from the combination of three inclusion levels of Cistus (50 v. 100 v. 200 g/kg of dry matter (DM)) and three inclusion levels of oil (0 v. 40 v. 80 g/kg of DM). The forage-to-concentrate ratio of the diets was 1 : 1. Longissimus muscle lipids were extracted, fractionated into neutral (NL) and polar lipid (PL) and FA methyl esters obtained and analyzed by GLC. The expression of genes encoding Δ5, Δ6 and Δ9 desaturases (fatty acid desaturase 1 (FADS1), fatty acid desaturase 2 (FADS2) and stearoyl CoA desaturase (SCD)) was determined. Intramuscular fat, NL and PL contents were not affected by oil or Cistus. Oil supplementation reduced (P<0.05) 16:0, c9-16:1, 17:0, c9-17:1 and c9-18:1 FA and increased (P<0.05) 18:2n-6, 18:3n-3 and the majority of biohydrogenation intermediates in NL. Cistus alone had few effects on FA of NL but interacted with oil (P<0.05) by increasing t10-18:1,t10,t12-18:2,t10,c12-18:2 and t7,c9-18:2. The t10-/t11-18:1 ratio increased with both Cistus and oil levels. The c9, t11-18:2 did not increase (P<0.05) with both oil and Cistus dietary inclusion. Oil reduced c9-16:1, 17:0, c9-17:1,c9-18:1, 20:4n-6, 22:4n-6 and 20:3n-9 proportions in PL, and increased 18:2n-6, 18:3n-3, 20:3n-3 and of most of the biohydrogenation intermediates. The Cistus had only minor effects on FA composition of PL. Cistus resulted in a reduction (P<0.05) of 20:5n-3 and 22:6n-3 in the meat PL. The expression level of SCD mRNA increased (P=0.015) with Cistus level, although a linear relationship with condensed tannins intake (P=0.11) could not be established. FADS1 mRNA expressed levels increased linearly (P=0.019) with condensed tannins intake. In summary, the inclusion of Cistus and oil in 1 : 1 forage-to-concentrate ratio diets resulted in a large increase in t10-18:1 and no increase in c9,t11-18:2 or n-3 long chain poor in polyunsaturated fatty acids in lamb meat.
1Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA),Avenida da Universidade Técnica,1300-477 Lisbon,Portugal.
A
A
Francisco
1Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA),Avenida da Universidade Técnica,1300-477 Lisbon,Portugal.
S P
SP
Alves
3Unidade Estratégica de Investigação e Serviços em Produção e Saúde Animal,Instituto Nacional de Investigação Agrária e Veterinária (INIAV),2005-048 Vale de Santarém,Portugal.
P V
PV
Portugal
1Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA),Avenida da Universidade Técnica,1300-477 Lisbon,Portugal.
V M R
VM
Pires
3Unidade Estratégica de Investigação e Serviços em Produção e Saúde Animal,Instituto Nacional de Investigação Agrária e Veterinária (INIAV),2005-048 Vale de Santarém,Portugal.
M T
MT
Dentinho
1Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA),Avenida da Universidade Técnica,1300-477 Lisbon,Portugal.
C M
CM
Alfaia
1Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA),Avenida da Universidade Técnica,1300-477 Lisbon,Portugal.
E
E
Jerónimo
1Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA),Avenida da Universidade Técnica,1300-477 Lisbon,Portugal.
J A M
JA
Prates
1Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA),Avenida da Universidade Técnica,1300-477 Lisbon,Portugal.
J
J
Santos-Silva
1Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA),Avenida da Universidade Técnica,1300-477 Lisbon,Portugal.
R J B
RJ
Bessa
D000821:Animal Feed / Q000032:analysis; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D029758:Cistus / Q000737:chemistry*; D004032:Diet; D019587:Dietary Supplements; D005227:Fatty Acids / Q000737:chemistry* / Q000378:metabolism; D005231:Fatty Acids, Unsaturated; D008043:Linseed Oil / Q000378:metabolism; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry*; D008297:Male; D008460:Meat / Q000032:analysis*; D010938:Plant Oils / Q000494:pharmacology*; D012756:Sheep / Q000502:physiology
D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D008055:Lipids; D010938:Plant Oils; D008043:Linseed Oil

               Trans-10 shift; biohydrogenation; fatty acid desaturases; lipid supplementation; tannins