Common Bean Leaves as a Source of Dietary Iron: Functional Test in an Iron-Deficient Rat Model.
Recent findings made by our group indicate that the iron content in Phaseolus vulgaris leaves is at least four times greater than in grains therefore, we evaluated the effect of supplementation with bean leaf (iron content of 275 mg/kg on a dry basis) in iron-deficient rats. Anemia was induced by feeding rats with an iron-deficient diet (IDD) for 11 days and iron-recovery diets were subsequently tested for 14 days using a normal diet, a 10 % bean leaf-supplemented IDD (BLSD) or a ferrous sulfate-supplemented IDD. Decreased levels of leukocytes (64 %), erythrocytes (30 %), lymphocytes (62 %), granulocytes (72 %), hematocrit (34 %), hemoglobin (35 %), and ferritin (34 %) were observed in the iron-deficient rats compared to the control rats. BLSD supplementation showed the highest recovery values relative to those recorded for control rats: leukocytes (40 %), erythrocytes (24 %), lymphocytes (33 %), granulocytes (88 %), hematocrit (17 %), and hemoglobin (18 %), suggesting that common bean leaves could be a good source of bioavailable iron with possible immunomodulatory effects.
Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Av de las Ciencias S/N., Juriquilla, Querétaro, C.P. 76230, México.
Mauricio
M
Martínez-Zavala
Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Unidad de Biotecnología, Campo Experimental Bajío, Celaya, Gto. 38110, México.
María Alejandra
MA
Mora-Avilés
Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Av de las Ciencias S/N., Juriquilla, Querétaro, C.P. 76230, México.
Miriam Aracely
MA
Anaya-Loyola
Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Unidad de Biotecnología, Campo Experimental Bajío, Celaya, Gto. 38110, México.
Horacio
H
Guzmán-Maldonado
Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Av de las Ciencias S/N., Juriquilla, Querétaro, C.P. 76230, México.
Araceli
A
Aguilera-Barreyro
Departamento de Biotecnología y Bioquímica, Centro de Investigación y Estudios Avanzados del IPN, Irapuato, Gto. 36821, México.
Alejandro
A
Blanco-Labra
Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Av de las Ciencias S/N., Juriquilla, Querétaro, C.P. 76230, México. tggasca@uaq.edu.mx.
Teresa
T
García-Gasca
D018798:Anemia, Iron-Deficiency / Q000097:blood / Q000517:prevention & control; D000818:Animals; D015415:Biomarkers / Q000097:blood; D019587:Dietary Supplements; D004195:Disease Models, Animal; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D005293:Ferritins / Q000097:blood / Q000172:deficiency; D005296:Ferrous Compounds / Q000008:administration & dosage; D006098:Granulocytes / Q000378:metabolism; D006400:Hematocrit; D006454:Hemoglobins / Q000172:deficiency / Q000378:metabolism; D019266:Iron, Dietary / Q000008:administration & dosage / Q000032:analysis*; D007962:Leukocytes / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism; D027805:Phaseolus / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar
D015415:Biomarkers; D005296:Ferrous Compounds; D006454:Hemoglobins; D019266:Iron, Dietary; C020748:ferrous sulfate; D005293:Ferritins
Common bean leaves; Complete blood count; Hemoglobin; Iron; Phaseolus vulgaris