Abca1 deficiency protects the heart against myocardial infarction-induced injury.
We explored the role of ATP-binding cassette transporter A1 (Abca1), in post-myocardial infarction (MI) cardiac injury.



In Abca1(-/-) mice, wild type (WT) mice, and WT mice transplanted with Abca1(-/-) or WT bone marrow, an MI was induced in vivo. Furthermore, an ex vivo MI was induced in isolated Abca1(-/-) and WT hearts.



Twenty-four hours and two weeks after in vivo MI induction, MI size was reduced in Abca1(-/-) (-58%, p = 0.007; -59%, p = 0.03) compared to WT. Ex vivo MI induction showed no effect of Abca1(-/-) on infarct size. Interestingly, two weeks after MI, Abca1(-/-) mice showed higher circulating levels of B-cells (+3.0 fold, p = 0.02) and T-cells (+4.2 fold, p = 0.002) compared to WT. Bone marrow-specific Abca1(-/-) tended to reduce infarct size (-43%, p = 0.12), suggesting a detrimental role for hematopoietic Abca1 after MI.



Although Abca1 has a protective role in atherosclerosis, it exerts detrimental effects on cardiac function after MI.
Department of Medicine, Division of Endocrinology, Leiden University Medical Center, Leiden, The Netherlands; Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, The Netherlands.
Mieke C
MC
Louwe
Division of Biopharmaceutics, Cluster BioTherapeutics, Leiden Academic Centre for Drug Research, Gorlaeus Laboratories, Leiden University, Leiden, The Netherlands.
Bart
B
Lammers
Department of Internal Medicine, Endocrinology, Diabetology and Nutrition, University of Geneva, Geneva, Switzerland.
Miguel A
MA
Frias
Division of Biopharmaceutics, Cluster BioTherapeutics, Leiden Academic Centre for Drug Research, Gorlaeus Laboratories, Leiden University, Leiden, The Netherlands.
Amanda C
AC
Foks
Division of Biopharmaceutics, Cluster BioTherapeutics, Leiden Academic Centre for Drug Research, Gorlaeus Laboratories, Leiden University, Leiden, The Netherlands.
Lidewij R
LR
de Leeuw
Division of Biopharmaceutics, Cluster BioTherapeutics, Leiden Academic Centre for Drug Research, Gorlaeus Laboratories, Leiden University, Leiden, The Netherlands.
Reeni B
RB
Hildebrand
Division of Biopharmaceutics, Cluster BioTherapeutics, Leiden Academic Centre for Drug Research, Gorlaeus Laboratories, Leiden University, Leiden, The Netherlands.
Johan
J
Kuiper
Department of Medicine, Division of Endocrinology, Leiden University Medical Center, Leiden, The Netherlands.
Johannes W A
JWA
Smit
Division of Biopharmaceutics, Cluster BioTherapeutics, Leiden Academic Centre for Drug Research, Gorlaeus Laboratories, Leiden University, Leiden, The Netherlands.
Theo J C
TJC
Van Berkel
Department of Internal Medicine, Endocrinology, Diabetology and Nutrition, University of Geneva, Geneva, Switzerland.
Richard W
RW
James
Division of Biopharmaceutics, Cluster BioTherapeutics, Leiden Academic Centre for Drug Research, Gorlaeus Laboratories, Leiden University, Leiden, The Netherlands.
Janine J
JJ
Geerling
Department of Medicine, Division of Endocrinology, Leiden University Medical Center, Leiden, The Netherlands; Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, The Netherlands.
Patrick C N
PCN
Rensen
Division of Biopharmaceutics, Cluster BioTherapeutics, Leiden Academic Centre for Drug Research, Gorlaeus Laboratories, Leiden University, Leiden, The Netherlands. Electronic address: m.eck@lacdr.leidenuniv.nl.
Miranda
M
Van Eck
D064286:ATP Binding Cassette Transporter 1 / Q000172:deficiency*; D000818:Animals; D050197:Atherosclerosis / Q000378:metabolism; D001402:B-Lymphocytes / Q000166:cytology; D016026:Bone Marrow Transplantation; D005260:Female; D006321:Heart / Q000503:physiopathology*; D007962:Leukocytes / Q000166:cytology; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009203:Myocardial Infarction / Q000235:genetics* / Q000378:metabolism / Q000517:prevention & control*
D064286:ATP Binding Cassette Transporter 1; C577833:Abca1 protein, mouse
Abca1 deficiency; Immune cells; Mice; Myocardial infarction