Placental abnormalities in equine pregnancies generated by SCNT from one donor horse.
Placental changes associated with SCNT have been described in several species, but little information is available in this area in the horse. We evaluated the ultrasonographic, gross, and histopathological characteristics of placentas from three successful and five unsuccessful equine SCNT pregnancies, established using cells from a single donor horse. Starting at approximately 6-month gestation, the pregnancies were monitored periodically using transrectal (TR) and transabdominal (TA) ultrasonography (US) to examine the placentas, fetal fluids, and fetuses. Of the five mares that aborted, one mare did so suddenly without any abnormal signs detected by US and four had enlarged umbilical vessels visible on TA-US before abortion. Placental edema (TR-US) and intravascular thrombi in the umbilical cords were seen (TA-US) in two of these four mares; one mare aborted shortly after acute placental separation was identified on TA-US. In three mares that delivered live foals, TA-US showed engorged allantoic vessels and enlarged umbilical vessels. Two of these mares had placental thickening visible on TR-US, interpreted as a sign of placentitis, that subsided after aggressive medical treatment. Seven of the eight placentas were submitted for gross and histopathological examinations after delivery. All placentas had some degree of edema, abnormally engorged allantoic vessels, and enlarged umbilical vessels. Placentitis, large allantoic vesicles, cystic pouches in the fetal part of the cord, and hemorrhages and thrombi in the umbilical vessels were detected only in placentas from mares that aborted. Equine pregnancies resulting from SCNT may be associated with placental pathologies that can be detected using ultrasonography. However, interpreting their severity is difficult. Although placental abnormalities have been observed in SCNT pregnancies in other species, to the best of our knowledge, placentitis has not been previously reported and may be an important complication of equine SCNT pregnancies, leading to pregnancy loss.
Department of Large Animal Sciences, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA. Electronic address: PozorM@ufl.edu.
Malgorzata A
MA
Pozor
Department of Infectious Diseases and Pathology, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA.
Barbara
B
Sheppard
Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, Texas, USA.
Katrin
K
Hinrichs
Department of Large Animal Sciences, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA.
Audrey A
AA
Kelleman
Department of Large Animal Sciences, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA.
Margo L
ML
Macpherson
Department of Large Animal Sciences, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA.
Erin
E
Runcan
Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, Texas, USA.
Young-Ho
YH
Choi
Department of Large Animal Sciences, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA.
Mouhamadou
M
Diaw
Equine Reproduction Center, Peterson & Smith Equine Hospital, Ocala, Florida, USA.
Philip M
PM
Mathews
D000034:Abortion, Veterinary / Q000235:genetics / Q000473:pathology; D000818:Animals; D019976:Cloning, Organism / Q000379:methods / Q000662:veterinary; D005260:Female; D006734:Horse Diseases / Q000235:genetics* / Q000473:pathology; D006736:Horses / Q000235:genetics; D053652:Nuclear Transfer Techniques / Q000009:adverse effects / Q000662:veterinary*; D010920:Placenta / Q000002:abnormalities* / Q000473:pathology; D010922:Placenta Diseases / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D011247:Pregnancy; D016216:Ultrasonography, Prenatal / Q000379:methods / Q000662:veterinary; D014470:Umbilical Cord / Q000473:pathology
Equine clone; Placenta; Placentitis; SCNT; Ultrasonographic