Brain-Derived Neurotrophic Factor Inhibits Intercellular Adhesion Molecule-1 Expression in Interleukin-1β-Treated Endothelial Cells.
Brain-derived neurotrophic factor (BDNF) enhances periodontal tissue regeneration. Tissue regeneration is characterized by inflammation, which directs the quality of tissue repair. The objective of this study is to propose the relevance of BDNF to inflammation. In this study, we investigated the effect of BDNF on intercellular adhesion molecule (ICAM)-1, which is an inflammatory marker, expressed in interleukin (IL)-1β-treated human microvascular endothelial cells (HMVECs). In addition, we studied the effect of BDNF on the adhesion of neutrophil-like differentiated HL-60 cells to HMVECs in a cell adhesion assay. We demonstrated that BDNF attenuates the IL-1β-induced ICAM-1 mRNA and protein expression. Treatment of HMVECs with IL-1β led to an increase in the number of adherent neutrophil-like HL-60 cells. BDNF significantly decreased the number of neutrophil-like HL-60 cells attached to HMVECs. In conclusion, BDNF may reduce excess inflammation through reduced neutrophil recruitment by regulating ICAM-1 expression.
Division of Applied Life Sciences, Department of Periodontal Medicine, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan. takepon@hiroshima-u.ac.jp.|Division of Frontier Medical Science, Department of Periodontal Medicine, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima, 734-8553, Japan. takepon@hiroshima-u.ac.jp.
Katsuhiro
K
Takeda
Division of Applied Life Sciences, Department of Periodontal Medicine, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.
Yusuke
Y
Obinata
Division of Applied Life Sciences, Department of Periodontal Medicine, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.
Akihiro
A
Konishi
Division of Applied Life Sciences, Department of Periodontal Medicine, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.
Mikihito
M
Kajiya
Division of Applied Life Sciences, Department of Periodontal Medicine, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.
Shinji
S
Matsuda
Division of Applied Life Sciences, Department of Periodontal Medicine, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.
Noriyoshi
N
Mizuno
Division of Applied Life Sciences, Department of Periodontal Medicine, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.
Shinya
S
Sasaki
Division of Applied Life Sciences, Department of Periodontal Medicine, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.
Tsuyoshi
T
Fujita
Division of Applied Life Sciences, Department of Periodontal Medicine, Institute of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan.
Hidemi
H
Kurihara
D015153:Blotting, Western; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects*; D018922:HL-60 Cells; D006801:Humans; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000378:metabolism*; D053583:Interleukin-1beta / Q000494:pharmacology*; D055806:Microvessels / Q000166:cytology; D009504:Neutrophils / Q000166:cytology / Q000276:immunology; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
D019208:Brain-Derived Neurotrophic Factor; D053583:Interleukin-1beta; D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1
Brain-derived neurotrophic factor; Interleukin-1β; Periodontal therapy; Vascular endothelial cells