Effect of Simulated Sanitizer Carryover on Recovery of Salmonella from Broiler Carcass Rinsates.
Numerous antimicrobial chemicals are currently utilized as processing aids with the aim of reducing pathogenic bacteria on processed poultry carcasses. Carryover of active sanitizer to a carcass rinse solution intended for recovery of viable pathogenic bacteria by regulatory agencies may cause false-negative results. This study was conducted to document the potential carryover effect of five sanitizing chemicals commonly used as poultry processing aids for broilers in a postchill dip. The effect of postdip drip time on the volume of sanitizer solution carryover was first determined by regression of data obtained from 10 carcasses. The five sanitizer solutions were diluted with buffered peptone water at 0-, 1-, and 5-min drip time equivalent volumes as determined by the regression analysis. These solutions were then spiked to 10(5) CFU/ml with a mixture of five nalidixic acid-resistant Salmonella enterica serovars, stored at 4°C for 24 h, and finally enumerated by plate count on brilliant green sulfa agar containing nalidixic acid. At the 0- and 1-min drip time equivalents, no Salmonella recovery was observed in three of the five sanitizers studied. At the 5-min drip time equivalent, one of these sanitizers still exhibited significant (P ≤ 0.05) bactericidal activity. These findings potentially indicate that the currently utilized protocol for the recovery of Salmonella bacteria from postchill sanitizer interventions may lead to false-negative results due to sanitizer carryover into the carcass rinsate.
U.S. National Poultry Research Center, Agricultural Research Service, U.S. Department of Agriculture, Athens, Georgia 30605, USA. gary.gamble@ars.usda.gov.
Gary R
GR
Gamble
U.S. National Poultry Research Center, Agricultural Research Service, U.S. Department of Agriculture, Athens, Georgia 30605, USA.
Mark E
ME
Berrang
U.S. National Poultry Research Center, Agricultural Research Service, U.S. Department of Agriculture, Athens, Georgia 30605, USA.
R Jeff
RJ
Buhr
U.S. National Poultry Research Center, Agricultural Research Service, U.S. Department of Agriculture, Athens, Georgia 30605, USA.
Arthur
A
Hinton
U.S. National Poultry Research Center, Agricultural Research Service, U.S. Department of Agriculture, Athens, Georgia 30605, USA.
Dianna V
DV
Bourassa
Office of Public Health Science, Food Safety and Inspection Service, U.S. Department of Agriculture, Building D, Suite 320, Fort Collins, Colorado 80526, USA.
John J
JJ
Johnston
U.S. National Poultry Research Center, Agricultural Research Service, U.S. Department of Agriculture, Athens, Georgia 30605, USA.
Kimberly D
KD
Ingram
U.S. National Poultry Research Center, Agricultural Research Service, U.S. Department of Agriculture, Athens, Georgia 30605, USA.
Eric S
ES
Adams
U.S. National Poultry Research Center, Agricultural Research Service, U.S. Department of Agriculture, Athens, Georgia 30605, USA.
Peggy W
PW
Feldner
D000818:Animals; D001419:Bacteria; D002645:Chickens / Q000382:microbiology*; D015169:Colony Count, Microbial; D005511:Food Handling; D005516:Food Microbiology; D012475:Salmonella / Q000187:drug effects