Protective effect of hydro-alcoholic extract of Ruta graveolens Linn. leaves on indomethacin and pylorus ligation-induced gastric ulcer in rats.
BACKGROUND
The search for an ideal and new antiulcer drug has been extended to herbals for novel molecules that decrease the incidence of relapse and afford better protection.


OBJECTIVE
The present study was designed to investigate the protective effect of hydro-alcoholic extract of Ruta graveolens (RGE) Linn. leaves on indomethacin (IND) and pylorus ligation-induced gastric ulcer in Wistar rats.


METHODS
The rats of all the six groups were deprived of food for 24 h. Then, the first group received 1 ml/kg/day p.o. of 1% carboxymethylcellulose calcium (CMC), second group 1 ml/kg/day p.o. of 1% CMC and third group 20 mg/kg/day p.o. of IND. Fourth and fifth groups received RGE 200 and 400 mg/kg/day p.o., respectively; while the sixth group 10 mg/kg/day p.o. omeprazole. After 30 min, last three groups received 20 mg/kg/day p.o. of IND also. All these treatments after food deprivation were repeated each day for 5 consecutive days. Pylorus ligation was performed on 6th day in last five groups. After 4 h, stomach by sacrifice of the rats was examined for ulcer index (UI) and gastric mucus. Gastric juice was assessed for acidity, pH and pepsin; while gastric tissues were assessed for thiobarbituric acid reactive substance (TBARS) and glutathione (GSH).


RESULTS
Fifth group showed significant decrease in UI (10.33 ± 0.67), TBARS (0.33 ± 0.03 mmol/mg), free acidity (48.78 ± 5.12 meq/l/100 g), total acidity (99.33 ± 9.31 meq/l/100 g), and pepsin activity (8.47 ± 0.41 μg/ml) levels while it showed significant increase in mucus (412.4 ± 21.6 μg/g), GSH (57.9 ± 4.8 mmol/mg) and pH (3.32 ± 0.27) compared to third group. Percent protection in RGE 400 mg was found to be 63.32 compared to indomethacin.


CONCLUSIONS
RGE possesses antiulcerogenic activity as it exhibits protective effect on gastric ulcer in rats.
Department of Pharmacy, Faculty of Pharmacy, Bioactive Research Laboratory, Integral University, Lucknow, Uttar Pradesh, India.
Mohd
M
Tarique
Department of Pharmacy, Faculty of Pharmacy, Bioactive Research Laboratory, Integral University, Lucknow, Uttar Pradesh, India.
Hefazat H
HH
Siddiqui
Department of Pharmacy, Faculty of Pharmacy, Bioactive Research Laboratory, Integral University, Lucknow, Uttar Pradesh, India. Electronic address: mohdkhushtar@gmail.com.
Mohd
M
Khushtar
Department of Pharmacy, Faculty of Pharmacy, Bioactive Research Laboratory, Integral University, Lucknow, Uttar Pradesh, India.
Md Azizur
MA
Rahman
Glutathione; Protective activity; Ruta graveolens; Thiobarbituric acid reactive substance; Ulcer index