Development and Evaluation of Species-Specific PCR for Detection of Nine Acinetobacter Species.
Molecular methods have the potential to improve the speed and accuracy of Acinetobacter species identification in clinical settings. The goal of this study is to develop species-specific PCR assays based on differences in the RNA polymerase beta-subunit gene (rpoB) to detect nine commonly isolated Acinetobacter species including Acinetobacter baumannii, Acinetobacter calcoaceticus, Acinetobacter pittii, Acinetobacter nosocomialis, Acinetobacter lwoffii, Acinetobacter ursingii, Acinetobacter bereziniae, Acinetobacter haemolyticus, and Acinetobacter schindleri. The sensitivity and specificity of these nine assays were measured using genomic DNA templates from 55 reference strains and from 474 Acinetobacter clinical isolates. The sensitivity of A. baumannii-specific PCR assay was 98.9%, and the sensitivity of species-specific PCR assays for all other species was 100%. The specificities of A. lwoffii- and A. schindleri-specific PCR were 97.8 and 98.9%, respectively. The specificity of species-specific PCR for all other tested Acinetobacter species was 100%. The lower limit of detection for the nine species-specific PCR assays developed in this study was 20 or 200 pg of genomic DNA from type strains of each species. The Acinetobacter species-specific PCR assay would be useful to determine the correct species among suggested candidate Acinetobacter species when conventional methods including MALDI-TOF MS identify Acinetobacter only to the genus level. The species-specific assay can be used to screen large numbers of clinical and environmental samples obtained for epidemiologic study of Acinetobacter for the presence of target species.
Research Center for Resistant Cells, Chosun University Medical School, Gwang-Ju, South Korea.
Xue Min
XM
Li
Research Center for Resistant Cells, Chosun University Medical School, Gwang-Ju, South Korea.
Ji Ae
JA
Choi
Department of Laboratory Medicine, Chosun University Medical School, Gwang-Ju, South Korea.
In Sun
IS
Choi
Korean Collection for Oral Microbiology and Department of Oral Biochemistry, College of Dentistry, Chosun University, Gwang-Ju, South Korea jkkook@chosun.ac.kr.
Joong Ki
JK
Kook
Korean Collection for Type Cultures, Biological Resources Center, Korea Research Institute of Bioscience and Biotechnology, Daejon, South Korea.
Young-Hyo
YH
Chang
Department of Laboratory Medicine, Chosun University Medical School, Gwang-Ju, South Korea.
Geon
G
Park
Research Center for Resistant Cells, Chosun University Medical School, Gwang-Ju, South Korea Department of Laboratory Medicine, Chosun University Medical School, Gwang-Ju, South Korea sjbjang@chosun.ac.kr.
Sook Jin
SJ
Jang
Department of Laboratory Medicine, Chosun University Medical School, Gwang-Ju, South Korea.
Seong Ho
SH
Kang
Department of Laboratory Medicine, Chosun University Medical School, Gwang-Ju, South Korea.
Dae Soo
DS
Moon
D000150:Acinetobacter / Q000235:genetics* / Q000302:isolation & purification*; D057230:Limit of Detection; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013045:Species Specificity
Acinetobacter; DNA Primers; Limit of Detection; Polymerase Chain Reaction; Sensitivity and Specificity; Species; rpoB gene