CRISPR Outsourcing: Commissioning IHF for Site-Specific Integration of Foreign DNA at the CRISPR Array.
In this issue of Molecular Cell, Nuñez et al. (2016) report that site-specific integration of foreign DNA into CRISPR loci by the Cas1-Cas2 integrase complex is promoted by a host factor, IHF (integration host factor), that binds and bends CRISPR leader DNA.
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA.
Yunzhou
Y
Wei
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA; Department of Genetics, University of Georgia, Athens, GA 30602, USA; Department of Microbiology, University of Georgia, Athens, GA 30602, USA. Electronic address: mterns@uga.edu.
Michael P
MP
Terns
D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D064130:CRISPR-Associated Proteins / Q000235:genetics*; D064112:Clustered Regularly Interspaced Short Palindromic Repeats; D004247:DNA; D004268:DNA-Binding Proteins / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D019426:Integrases / Q000235:genetics; D020416:Outsourced Services
D001426:Bacterial Proteins; D064130:CRISPR-Associated Proteins; D004268:DNA-Binding Proteins; D004247:DNA; D019426:Integrases