Biopolymers Regulate Silver Nanoparticle under Microwave Irradiation for Effective Antibacterial and Antibiofilm Activities.
In the current study, facile synthesis of carboxymethyl cellulose (CMC) and sodium alginate capped silver nanoparticles (AgNPs) was examined using microwave radiation and aniline as a reducing agent. The biopolymer matrix embedded nanoparticles were synthesized under various experimental conditions using different concentrations of biopolymer (0.5, 1, 1.5, 2%), volumes of reducing agent (50, 100, 150 μL), and duration of heat treatment (30 s to 240 s). The synthesized nanoparticles were analyzed by scanning electron microscopy, UV-Vis spectroscopy, X-ray diffraction, and Fourier transform infrared spectroscopy for identification of AgNPs synthesis, crystal nature, shape, size, and type of capping action. In addition, the significant antibacterial efficacy and antibiofilm activity of biopolymer capped AgNPs were demonstrated against different bacterial strains, Staphylococcus aureus MTCC 740 and Escherichia coli MTCC 9492. These results confirmed the potential for production of biopolymer capped AgNPs grown under microwave irradiation, which can be used for industrial and biomedical applications.
Department of Biotechnology, School of Bioengineering, SRM University, Kancheepuram, Tamil Nadu, India.
Palaniyandi
P
Velusamy
Department of Chemical Engineering, Ming Chi University of Technology, Taishan, Taipei, Taiwan.
Chia-Hung
CH
Su
Department of Biotechnology, School of Bioengineering, SRM University, Kancheepuram, Tamil Nadu, India.
Govindarajan
G
Venkat Kumar
Department of Biotechnology, School of Bioengineering, SRM University, Kancheepuram, Tamil Nadu, India.
Shritama
S
Adhikary
Department of Inorganic Chemistry, University of Madras, Guindy Campus, Chennai, Tamil Nadu, India.
Kannaiyan
K
Pandian
Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, Kangar, Perlis, Malaysia.|School of Bioprocess Engineering, Universiti Malaysia Perlis, Arau, Perlis, Malaysia.
Subash C B
SC
Gopinath
Department of Oral Biology and Biomedical Sciences, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia.
Yeng
Y
Chen
Department of Biological Engineering, College of Engineering, Inha University, Incheon, Republic of Korea.
Periasamy
P
Anbu
D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000633:toxicity; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D001704:Biopolymers / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects / Q000502:physiology; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000528:radiation effects / Q000633:toxicity; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D008872:Microwaves; D010316:Particle Size; D012834:Silver / Q000737:chemistry*; D013053:Spectrophotometry; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects / Q000502:physiology; D014961:X-Ray Diffraction
D000900:Anti-Bacterial Agents; D001704:Biopolymers; D012834:Silver