BK and JC virus infections in healthy patients compared to kidney transplant recipients in Tunisia.
The human polyomaviruses BKPyV and JCPyV are members of Polyomaviridae family and after primary infections they persist as latent infection especially in the kidneys. BKVPy reactivation is mainly related to a renal nephropathy and JCV reactivation can induce the progressive multifocal leukoencephalopathy. The aim of this study was to investigate and to compare the presence of BKPyV and JCPyV in urine and plasma samples from immunocompromised and immunocompetent groups. The viral detection and quantification was done by a real time PCR from 100 healthy individuals and from 72 kidney transplanted patients (KTx) enrolled in a prospective study. Polyomavirus DNA urinary shedding was identified in 19% of healthy person, BKPyV in 6%; JCPyV more frequent in 13%. No individuals in this group developed polyomavirus viremia. BKPyV and JCPyV viruria was seen in 36% and 28% of KTx respectively, and 11% had a concomitant BKPyV and JCPyV viruria. Only BKPy viremia was detected in 5.5% of the KTx. In healthy persons, JCPyV shedding was associated with older individuals. However, in KTx, BKPyV was associated with younger age and male gender. No significant association was found between the patient's origin and BKPyV or JCPyV infection. In conclusion and consisting with previous reports, BKPyV and JCPyV prevalence and urinary loads were significantly higher in immunosuppressed compared to non-immunosuppressed individuals. In Addition and by contrast to KTx, JCPyV was more frequent than BKPyV in healthy individuals. Furthermore, the shedding of both polyomaviruses was differently associated with the age and the sex according to each population.
Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives, LR-99ES27, Faculté de Pharmacie, Avenue Avicenne, 5000, Monastir, Tunisia. Electronic address: han_boukoum@yahoo.fr.
Hanen
H
Boukoum
Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives, LR-99ES27, Faculté de Pharmacie, Avenue Avicenne, 5000, Monastir, Tunisia.
Imen
I
Nahdi
Service de Néphrologie, Hôpital Sahloul, 4054, Sousse, Tunisia.
Wissal
W
Sahtout
Service de Néphrologie, Hôpital Fattouma Borguiba, Avenue du 1er Juin 5000, Monastir, Tunisia.
Habib
H
Skiri
Laboratoire de Virologie, CHU de Montpellier, Université de Montpellier I, hôpital Saint-Éloi, 80, avenue A. Fliche, 34295, Montpellier Cedex 5, France.
Michel
M
Segondy
Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives, LR-99ES27, Faculté de Pharmacie, Avenue Avicenne, 5000, Monastir, Tunisia.
Mahjoub
M
Aouni
D000293:Adolescent; D000328:Adult; D001739:BK Virus / Q000302:isolation & purification*; D002353:Carrier State / Q000453:epidemiology / Q000821:virology; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D016867:Immunocompromised Host; D007577:JC Virus / Q000302:isolation & purification*; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D010949:Plasma / Q000821:virology; D027601:Polyomavirus Infections / Q000453:epidemiology* / Q000821:virology*; D015995:Prevalence; D011446:Prospective Studies; D060888:Real-Time Polymerase Chain Reaction; D066027:Transplant Recipients; D014416:Tunisia / Q000453:epidemiology; D014556:Urine / Q000821:virology; D019562:Viral Load; D017201:Virus Shedding; D055815:Young Adult
D004279:DNA, Viral
BK virus; JC virus; Transplantation; Viral excretion