Cutinostatin B as a New Cutinase Inhibitor Produced by Actinomycete.
Cutinostatin B was purified from a culture filtrate of a strain No. 3112-4 of an actinomycete which had been isolated from a soil sample collected in Setagaya-ku, Tokyo, Japan. The structure of this compound was determined mainly by 2D NMR experiments to be N(α)-[N(α)-(2,3-dihydroxybenzoyl)-ornithyl]-N(δ)-hydroxyornithine-δ-lactum. Cutinostatin B inhibited the cutinase activity of Cladosporium fulvum when using β-naphthyl caproate as the substrate with an IC50 value of 28.9 μm (11.0 μg/ml), and inhibited 95% when using crude apple cutin as the substrate with a concentration of 263 μM (100 μg/ml).
a Department of Agricultural Chemistry, Faculty of Agriculture , Tamagawa University , Machidashi, Tokyo 194 , Japan.
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Higashi
a Department of Agricultural Chemistry, Faculty of Agriculture , Tamagawa University , Machidashi, Tokyo 194 , Japan.
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Hirose
a Department of Agricultural Chemistry, Faculty of Agriculture , Tamagawa University , Machidashi, Tokyo 194 , Japan.
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Hori
a Department of Agricultural Chemistry, Faculty of Agriculture , Tamagawa University , Machidashi, Tokyo 194 , Japan.
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Ishiyama
cuticular layer; cutin; cutinase; enzyme inhibitor; phytopathogenic fungi