A Microdosimetric Study of Electropulsation on Multiple Realistically Shaped Cells: Effect of Neighbours.
Over the past decades, the effects of ultrashort-pulsed electric fields have been used to investigate their action in many medical applications (e.g. cancer, gene electrotransfer, drug delivery, electrofusion). Promising aspects of these pulses has led to several in vitro and in vivo experiments to clarify their action. Since the basic mechanisms of these pulses have not yet been fully clarified, scientific interest has focused on the development of numerical models at different levels of complexity: atomic (molecular dynamic simulations), microscopic (microdosimetry) and macroscopic (dosimetry). The aim of this work is to demonstrate that, in order to predict results at the cellular level, an accurate microdosimetry model is needed using a realistic cell shape, and with their position and packaging (cell density) characterised inside the medium.
Center for Life Nano Science at Sapienza, Istituto Italiano di Tecnologia, Rome, Italy.|Department of Information Engineering, Electronics and Telecommunication (DIET), Italian Inter-University Centre of Electromagnetic Fields and Bio-Systems (ICEmB), University of Rome "La Sapienza", 00184, Rome, Italy.
Agnese
A
Denzi
Department of Information Engineering, Electronics and Telecommunication (DIET), Italian Inter-University Centre of Electromagnetic Fields and Bio-Systems (ICEmB), University of Rome "La Sapienza", 00184, Rome, Italy.
Francesca
F
Camera
Division of Health Protection Technologies, ENEA-Italian National Agency for New Technologies, Energy and Sustainable Economic Development, 00123, Rome, Italy.|Vectorology and Anticancer Therapies, UMR 8203, CNRS, Gustave Roussy, Univ. Paris-Sud, Université Paris-Saclay, 94805, Villejuif, France.
Caterina
C
Merla
Division of Health Protection Technologies, ENEA-Italian National Agency for New Technologies, Energy and Sustainable Economic Development, 00123, Rome, Italy.
Barbara
B
Benassi
Division of Health Protection Technologies, ENEA-Italian National Agency for New Technologies, Energy and Sustainable Economic Development, 00123, Rome, Italy.
Claudia
C
Consales
Department of Information Engineering, Electronics and Telecommunication (DIET), Italian Inter-University Centre of Electromagnetic Fields and Bio-Systems (ICEmB), University of Rome "La Sapienza", 00184, Rome, Italy.
Alessandra
A
Paffi
Department of Information Engineering, Electronics and Telecommunication (DIET), Italian Inter-University Centre of Electromagnetic Fields and Bio-Systems (ICEmB), University of Rome "La Sapienza", 00184, Rome, Italy.
Francesca
F
Apollonio
Department of Information Engineering, Electronics and Telecommunication (DIET), Italian Inter-University Centre of Electromagnetic Fields and Bio-Systems (ICEmB), University of Rome "La Sapienza", 00184, Rome, Italy. liberti@diet.uniroma1.it.
Micaela
M
Liberti
D045744:Cell Line, Tumor; D002462:Cell Membrane; D048430:Cell Shape; D018274:Electroporation / Q000379:methods*; D006801:Humans; D008954:Models, Biological; D008962:Models, Theoretical
Electroporation; Mesodosimetry; Microdosimetry; Nanosecond and microsecond pulsed electric fields; Shielding effect