The antiviral effects of acteoside and the underlying IFN-γ-inducing action.
There are many herbal teas that are found in nature that may be effective at treating the symptoms and also shortening the duration of viral infections. When combating viral infections, T lymphocytes are an indispensable part of human acquired immunity. However, studies on the use of natural products in stimulating lymphocyte-mediated interferon-gamma (IFN-γ) production are very limited. In this study, we found that acteoside, a natural phenylpropanoid glycoside from Kuding Tea, enhanced IFN-γ production in mouse lymphocytes in a dose-dependent manner, particularly in the CD4+ and CD8+ subsets of T lymphocytes. To this end, we suggest that the antiviral activity of acteoside was highly correlated to its inducing ability of IFN-γ production. Mechanistically, the activation of T-bet enhanced the promoter of IFN-γ and subsequently resulted in an increased IFN-γ production in T cells. Collectively, we have found a natural product with the capacity to selectively enhance mouse T cell IFN-γ production. Given the role of IFN-γ in the immune system, further studies to clarify the role of acteoside in inducing IFN-γ and prevention of viral infection are needed.
Department of Pharmacy, Shenzhen Key Laboratory of Novel Natural Health Care Products, Innovation Platform for Natural Small Molecule Drugs, Engineering Laboratory of Shenzhen Natural Small Molecule Innovative Drugs, School of Medicine, College of Life Science, Shenzhen University, Shenzhen 518060, P. R. China. jzhanghappy@szu.edu.cn hezhendan@126.com and School of Chinese Medicine, Hong Kong Baptist University, Hong Kong SAR, P. R. China. zhanghj@hkbu.edu.hk.
Xun
X
Song
Department of Pharmacy, Shenzhen Key Laboratory of Novel Natural Health Care Products, Innovation Platform for Natural Small Molecule Drugs, Engineering Laboratory of Shenzhen Natural Small Molecule Innovative Drugs, School of Medicine, College of Life Science, Shenzhen University, Shenzhen 518060, P. R. China. jzhanghappy@szu.edu.cn hezhendan@126.com.
Jiang
J
He
Department of Pharmacy, Shenzhen Key Laboratory of Novel Natural Health Care Products, Innovation Platform for Natural Small Molecule Drugs, Engineering Laboratory of Shenzhen Natural Small Molecule Innovative Drugs, School of Medicine, College of Life Science, Shenzhen University, Shenzhen 518060, P. R. China. jzhanghappy@szu.edu.cn hezhendan@126.com.
Hong
H
Xu
Department of Pharmacy, Shenzhen Key Laboratory of Novel Natural Health Care Products, Innovation Platform for Natural Small Molecule Drugs, Engineering Laboratory of Shenzhen Natural Small Molecule Innovative Drugs, School of Medicine, College of Life Science, Shenzhen University, Shenzhen 518060, P. R. China. jzhanghappy@szu.edu.cn hezhendan@126.com.
Xiao-Peng
XP
Hu
Department of Pharmacy, Shenzhen Key Laboratory of Novel Natural Health Care Products, Innovation Platform for Natural Small Molecule Drugs, Engineering Laboratory of Shenzhen Natural Small Molecule Innovative Drugs, School of Medicine, College of Life Science, Shenzhen University, Shenzhen 518060, P. R. China. jzhanghappy@szu.edu.cn hezhendan@126.com.
Xu-Li
XL
Wu
Department of Pharmacy, Shenzhen Key Laboratory of Novel Natural Health Care Products, Innovation Platform for Natural Small Molecule Drugs, Engineering Laboratory of Shenzhen Natural Small Molecule Innovative Drugs, School of Medicine, College of Life Science, Shenzhen University, Shenzhen 518060, P. R. China. jzhanghappy@szu.edu.cn hezhendan@126.com.
Hai-Qiang
HQ
Wu
Department of Pharmacy, Shenzhen Key Laboratory of Novel Natural Health Care Products, Innovation Platform for Natural Small Molecule Drugs, Engineering Laboratory of Shenzhen Natural Small Molecule Innovative Drugs, School of Medicine, College of Life Science, Shenzhen University, Shenzhen 518060, P. R. China. jzhanghappy@szu.edu.cn hezhendan@126.com.
Li-Zhong
LZ
Liu
Department of Pharmacy, Shenzhen Key Laboratory of Novel Natural Health Care Products, Innovation Platform for Natural Small Molecule Drugs, Engineering Laboratory of Shenzhen Natural Small Molecule Innovative Drugs, School of Medicine, College of Life Science, Shenzhen University, Shenzhen 518060, P. R. China. jzhanghappy@szu.edu.cn hezhendan@126.com.
Cheng-Hui
CH
Liao
The First Affiliated Hospital of Kunming Medical University, Kunming 650032, P. R. China.
Yong
Y
Zeng
The First Affiliated Hospital of Kunming Medical University, Kunming 650032, P. R. China.
Yan
Y
Li
Department of Pharmacy, Shenzhen Key Laboratory of Novel Natural Health Care Products, Innovation Platform for Natural Small Molecule Drugs, Engineering Laboratory of Shenzhen Natural Small Molecule Innovative Drugs, School of Medicine, College of Life Science, Shenzhen University, Shenzhen 518060, P. R. China. jzhanghappy@szu.edu.cn hezhendan@126.com.
Yue
Y
Hao
Department of Pharmacy, Shenzhen Key Laboratory of Novel Natural Health Care Products, Innovation Platform for Natural Small Molecule Drugs, Engineering Laboratory of Shenzhen Natural Small Molecule Innovative Drugs, School of Medicine, College of Life Science, Shenzhen University, Shenzhen 518060, P. R. China. jzhanghappy@szu.edu.cn hezhendan@126.com.
Chen-Shu
CS
Xu
Department of Pharmacy, Shenzhen Key Laboratory of Novel Natural Health Care Products, Innovation Platform for Natural Small Molecule Drugs, Engineering Laboratory of Shenzhen Natural Small Molecule Innovative Drugs, School of Medicine, College of Life Science, Shenzhen University, Shenzhen 518060, P. R. China. jzhanghappy@szu.edu.cn hezhendan@126.com.
Long
L
Fan
Department of Pharmacy, Shenzhen Key Laboratory of Novel Natural Health Care Products, Innovation Platform for Natural Small Molecule Drugs, Engineering Laboratory of Shenzhen Natural Small Molecule Innovative Drugs, School of Medicine, College of Life Science, Shenzhen University, Shenzhen 518060, P. R. China. jzhanghappy@szu.edu.cn hezhendan@126.com.
Jian
J
Zhang
School of Chinese Medicine, Hong Kong Baptist University, Hong Kong SAR, P. R. China. zhanghj@hkbu.edu.hk.
Hong-Jie
HJ
Zhang
Department of Pharmacy, Shenzhen Key Laboratory of Novel Natural Health Care Products, Innovation Platform for Natural Small Molecule Drugs, Engineering Laboratory of Shenzhen Natural Small Molecule Innovative Drugs, School of Medicine, College of Life Science, Shenzhen University, Shenzhen 518060, P. R. China. jzhanghappy@szu.edu.cn hezhendan@126.com.
Zhen-Dan
ZD
He
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001688:Biological Products / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004306:Dose-Response Relationship, Immunologic; D005960:Glucosides / Q000494:pharmacology*; D006027:Glycosides / Q000494:pharmacology; D053118:Influenza A Virus, H1N1 Subtype / Q000187:drug effects; D007371:Interferon-gamma / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010636:Phenols / Q000494:pharmacology*; D011401:Promoter Regions, Genetic; D000067996:RAW 264.7 Cells; D020825:T-Box Domain Proteins / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism; D000069459:Teas, Herbal / Q000032:analysis; D000098815:T-bet Transcription Factor; D059808:Polyphenols
D000998:Antiviral Agents; D001688:Biological Products; D005960:Glucosides; D006027:Glycosides; D007371:Interferon-gamma; D010636:Phenols; D020825:T-Box Domain Proteins; D000069459:Teas, Herbal; D000098815:T-bet Transcription Factor; C058956:acteoside; D059808:Polyphenols