The pre-mir-27a variant rs895819 may contribute to type 2 diabetes mellitus susceptibility in an Iranian cohort.
OBJECTIVE
The study was aimed at investigating the association between hsa-mir-27a polymorphism rs895819 (T/C) and type 2 diabetes mellitus (T2DM) susceptibility in a large Iranian cohort.


METHODS
In this case-control study, the investigated population consisted of T2DM patients (n = 204) and sex- and age-matched controls (n = 209). We used the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) for genotyping.


RESULTS
We observed significant differences between T2DM patients and controls for weight (p = 0.002), BMI (p < 0.001), systolic blood pressure (p < 0.001), diastolic blood pressure (p < 0.001), fasting plasma glucose (p < 0.001), triglyceride (p = 0.004) and LDL cholesterol (p = 0.051). Moreover, we found that genotype distributions were significantly different between groups (p < 0.05) and that the rs895819-C allele is more frequent in controls (p = 0.030, OR = 0.72, 95 % CI 0.53-0.97).


CONCLUSIONS
Our study shows that rs895819 in hsa-mir-27a is associated with T2DM susceptibility and that the C allele conveyed a protective role against T2DM. Larger multicentric and specific functional studies will be necessary to obtain a deeper comprehension of the role of rs895819 and hsa-mir-27a and how they are involved in the development of diabetes.
Medical Genetics Department, School of Medicine, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
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H
Ghaedi
Medical Biotechnology Department, Pasteur Institute of Iran, Tehran, Iran.
M
M
Tabasinezhad
Clinical Biochemistry Department, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
B
B
Alipoor
Medical Genetics Department, School of Medicine, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
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F
Shokri
Medical Genetics Department, School of Medicine, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
A
A
Movafagh
Medical Genetics Department, School of Medicine, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
R
R
Mirfakhraie
Medical Genetics Department, School of Medicine, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. davood_omrani@yahoo.co.uk.
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MD
Omrani
Gene Expression - Microarrays Laboratory, Bambino Gesù Children's Hospital-IRCCS, Viale di San Paolo 15, 00146, Rome, Italy. andrea.masotti@opbg.net.
A
A
Masotti
D020029:Base Pairing; D001483:Base Sequence; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D015331:Cohort Studies; D019295:Computational Biology; D003924:Diabetes Mellitus, Type 2 / Q000209:etiology* / Q000473:pathology; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D007492:Iran; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012307:Risk Factors
D015415:Biomarkers; C531388:MIRN27 microRNA, human; D035683:MicroRNAs
MicroRNA; Single nucleotide polymorphism; Type 2 diabetes mellitus; hsa-mir-27a