Comparison of small biopsy specimens and surgical specimens for the detection of EGFR mutations and EML4-ALK in non-small-cell lung cancer.
Epidermal growth factor receptor (EGFR) mutations and anaplastic lymphoma kinase (ALK) fusion genes represent novel oncogenes that are associated with non-small-cell lung cancers (NSCLC). The feasibility of detecting EGFR mutations and ALK fusion genes in small biopsy specimens or surgical specimens was determined. Of the 721 NSCLC patients, a total of 305 cases were positive for EGFR mutations (42.3%). The rate of EGFR mutations in women was significantly higher than that in men. Histologically, the EGFR mutation rate in adenocarcinomas was significantly higher than that in squamous cell carcinomas. No difference in the EGFR mutation rate was observed between surgical specimens (42.1%) and small biopsy specimens (42.4%), which indicated that the EGFR mutation ratios in surgical specimens and small biopsy specimens were not different. In 385 NSCLC patients, 26 cases were positive for EML4-ALK (6.8%). However, 11.7% of the surgical specimens were EML4-ALK-positive, whereas the positive proportion in the small biopsy specimens was only 4.7%, which indicated that EML4-ALK-positive rate in the surgical specimens was significantly higher than that in the small biopsy specimens. Detection of EGFR gene mutations was feasible in small biopsy specimens, and screening for EML4-ALK expression in small biopsy specimens can be used to guide clinical treatments.
Department of Pathology, Xiangya Hospital, Central South University, Changsha, Hunan, 410078, China.|Department of Pathology, School of Basic Medicine, Central South University, Changsha, Hunan, 410078, China.
DeSheng
D
Xiao
Department of Pathology, Xiangya Hospital, Central South University, Changsha, Hunan, 410078, China.|Department of Pathology, School of Basic Medicine, Central South University, Changsha, Hunan, 410078, China.
Can
C
Lu
Department of Pathology, Xiangya Hospital, Central South University, Changsha, Hunan, 410078, China.|Department of Pathology, School of Basic Medicine, Central South University, Changsha, Hunan, 410078, China.
Wei
W
Zhu
Department of Pathology, Xiangya Hospital, Central South University, Changsha, Hunan, 410078, China.|Department of Pathology, School of Basic Medicine, Central South University, Changsha, Hunan, 410078, China.
QiuYan
Q
He
Department of Pathology, Xiangya Hospital, Central South University, Changsha, Hunan, 410078, China.|Department of Pathology, School of Basic Medicine, Central South University, Changsha, Hunan, 410078, China.
Yong
Y
Li
Department of Pathology, Xiangya Hospital, Central South University, Changsha, Hunan, 410078, China.|Department of Pathology, School of Basic Medicine, Central South University, Changsha, Hunan, 410078, China.
ChunYan
C
Fu
Department of Pathology, Xiangya Hospital, Central South University, Changsha, Hunan, 410078, China.|Department of Pathology, School of Basic Medicine, Central South University, Changsha, Hunan, 410078, China.
JianHua
J
Zhou
Center for Medicine Research, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.
Shuang
S
Liu
Cancer Research Institute, School of Basic Medicine, Central South University, Changsha, Hunan, 410078, China.|Key Laboratory of Carcinogenesis and Cancer Invasion (Central South University), Ministry of Education, Hunan, 410078, China.|Key Laboratory of Carcinogenesis (Central South University), Ministry of Health, Hunan, 410078, China.|Center for Medicine Research, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China.
YongGuang
Y
Tao
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D066246:ErbB Receptors / Q000235:genetics*; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009154:Mutation / Q000235:genetics*; D015514:Oncogene Proteins, Fusion / Q000235:genetics*; D055815:Young Adult
C522504:EML4-ALK fusion protein, human; D015514:Oncogene Proteins, Fusion; C512478:EGFR protein, human; D066246:ErbB Receptors
EGFR; EML4-ALK; non-small-cell lung carcinoma; small biopsy specimens; surgical specimens