New Aspercryptins, Lipopeptide Natural Products, Revealed by HDAC Inhibition in Aspergillus nidulans.
Unlocking the biochemical stores of fungi is key for developing future pharmaceuticals. Through reduced expression of a critical histone deacetylase in Aspergillus nidulans, increases of up to 100-fold were observed in the levels of 15 new aspercryptins, recently described lipopeptides with two noncanonical amino acids derived from octanoic and dodecanoic acids. In addition to two NMR-verified structures, MS/MS networking helped uncover an additional 13 aspercryptins. The aspercryptins break the conventional structural orientation of lipopeptides and appear "backward" when compared to known compounds of this class. We have also confirmed the 14-gene aspercryptin biosynthetic gene cluster, which encodes two fatty acid synthases and several enzymes to convert saturated octanoic and dodecanoic acid to α-amino acids.
Department of Molecular Biosciences, ‡Department of Chemistry and §Feinberg School of Medicine, Northwestern University , Evanston, Illinois 60208, United States.|Department of Genetics, ⊥Department of Bacteriology, and #Department of Medical Microbiology and Immunology University of Wisconsin , Madison, Wisconsin 53706, United States.
Matthew T
MT
Henke
Department of Molecular Biosciences, ‡Department of Chemistry and §Feinberg School of Medicine, Northwestern University , Evanston, Illinois 60208, United States.|Department of Genetics, ⊥Department of Bacteriology, and #Department of Medical Microbiology and Immunology University of Wisconsin , Madison, Wisconsin 53706, United States.
Alexandra A
AA
Soukup
Department of Molecular Biosciences, ‡Department of Chemistry and §Feinberg School of Medicine, Northwestern University , Evanston, Illinois 60208, United States.|Department of Genetics, ⊥Department of Bacteriology, and #Department of Medical Microbiology and Immunology University of Wisconsin , Madison, Wisconsin 53706, United States.
Anthony W
AW
Goering
Department of Molecular Biosciences, ‡Department of Chemistry and §Feinberg School of Medicine, Northwestern University , Evanston, Illinois 60208, United States.|Department of Genetics, ⊥Department of Bacteriology, and #Department of Medical Microbiology and Immunology University of Wisconsin , Madison, Wisconsin 53706, United States.
Ryan A
RA
McClure
Department of Molecular Biosciences, ‡Department of Chemistry and §Feinberg School of Medicine, Northwestern University , Evanston, Illinois 60208, United States.|Department of Genetics, ⊥Department of Bacteriology, and #Department of Medical Microbiology and Immunology University of Wisconsin , Madison, Wisconsin 53706, United States.
Regan J
RJ
Thomson
Department of Molecular Biosciences, ‡Department of Chemistry and §Feinberg School of Medicine, Northwestern University , Evanston, Illinois 60208, United States.|Department of Genetics, ⊥Department of Bacteriology, and #Department of Medical Microbiology and Immunology University of Wisconsin , Madison, Wisconsin 53706, United States.
Nancy P
NP
Keller
Department of Molecular Biosciences, ‡Department of Chemistry and §Feinberg School of Medicine, Northwestern University , Evanston, Illinois 60208, United States.|Department of Genetics, ⊥Department of Bacteriology, and #Department of Medical Microbiology and Immunology University of Wisconsin , Madison, Wisconsin 53706, United States.
Neil L
NL
Kelleher
D001233:Aspergillus nidulans / Q000201:enzymology / Q000378:metabolism*; D002853:Chromatography, Liquid; D006655:Histone Deacetylases / Q000378:metabolism*; D055432:Metabolomics; D005810:Multigene Family; D009842:Oligopeptides / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D053719:Tandem Mass Spectrometry
D009842:Oligopeptides; C000611661:aspercryptin; D006655:Histone Deacetylases