MDG-1, an Ophiopogon polysaccharide, alleviates hyperlipidemia in mice based on metabolic profile of bile acids.
Hyperlipidemia is a chronic metabolic disorder with systemic complications that is prevalent worldwide. MDG-1, a water-soluble β-d-fructan polysaccharide from Ophiopogon japonicas has potent hypolipidemic and weight-control effects. The present study aimed to investigate the effects of MDG-1 on lipid metabolic disorders in diet-induced obese mice based on the metabolic profile of bile acids. C57BL/6 mice were treated with a low-fat diet, high-fat diet or high fat mixed with 1‰ (w/w) MDG-1 diet for 12 weeks. The results showed that MDG-1 inhibited body weight gain and lowered serum and liver total cholesterol contents in obese mice. In addition, MDG-1 could adsorb bile acids in the gut lumen and reduce their reabsorption, thus promoting cholesterol catabolism. Furthermore, MDG-1 inhibited the expression of the farnesoid X receptor, but activated the liver X receptor. Our findings shed new light on the mechanism of MDG-1 in the control of lipids.
Engineering Research Center of Modern Preparation Technology of TCM, Ministry of Education, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, PR China.
Linlin
L
Shi
Institute of Chinese Material Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, PR China.
Jie
J
Wang
Engineering Research Center of Modern Preparation Technology of TCM, Ministry of Education, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, PR China. Electronic address: amoness@163.com.
Yuan
Y
Wang
Engineering Research Center of Modern Preparation Technology of TCM, Ministry of Education, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, PR China. Electronic address: fyi_shutcm@163.com.
Yi
Y
Feng
D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism*; D001835:Body Weight / Q000187:drug effects; D059305:Diet, High-Fat / Q000009:adverse effects; D005243:Feces / Q000737:chemistry; D005786:Gene Expression Regulation / Q000187:drug effects; D006949:Hyperlipidemias / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism*; D050356:Lipid Metabolism / Q000187:drug effects; D008055:Lipids / Q000097:blood; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D055432:Metabolomics; D051379:Mice; D008810:Mice, Inbred C57BL; D031441:Ophiopogon / Q000737:chemistry*; D011134:Polysaccharides / Q000494:pharmacology* / Q000627:therapeutic use
D001647:Bile Acids and Salts; D008055:Lipids; D011134:Polysaccharides; C517472:polysaccharide MDG-1
Bile acids; Dyslipidemia; Nuclear receptors; Polysaccharide; UPLC–MS