Selective capture and rapid identification of Panax notoginseng metabolites in rat faeces by the integration of magnetic molecularly imprinted polymers and high-performance liquid chromatography coupled with orbitrap mass spectrometry.
In the present work, an advanced pretreatment method magnetic molecular imprinted polymers-dispersive solid phase extraction (MMIPs-DSPE) combined with the high sensitivity LTQ-Orbitrap mass spectrometry was applied to the complicated metabolites analysis of Traditional Chinese Medicines (TCMs) in complex matrices. The ginsenoside Rb1 magnetic molecular imprinted polymers (Rb1-MMIPs) were successfully synthesized for specific recognition and selective enrichment of Panax notoginseng saponin metabolites in rat faeces. The polymers were prepared by using Fe3O4@SiO2 as the supporting material, APTES as the functional monomer and TEOS as the cross-linker. The Rb1-MMIPs showed quick separation (10.8 emu/g), large adsorption capacity (636μmol/g), high selectivity and fast binding kinetics (25min). Dispersion solid-phase extraction using Rb1-MMIPs (Rb1-MMIPs-DSPE) integrated with LTQ-Orbitrap MS was applied to fish out and identify saponin metabolites from rat faeces, and totally 58 related compounds were detected within 20min, including 26 PPD-group and 32 PPT-group notoginsenoside metabolites. Parallel tests showed that Rb1-MMIPs-DSPE obtained the lowest matrix effects of 0.98-14.84% and captured the largest number of structural analogues compared with traditional pretreatment methods organic solvent extraction (OSE) and solid phase extraction (SPE).
School of Pharmacy, Nanjing Medical University, Nanjing 210029, PR China.
Qizhi
Q
Cai
School of Pharmacy, Nanjing Medical University, Nanjing 210029, PR China.
Zaiyue
Z
Yang
School of Pharmacy, Nanjing Medical University, Nanjing 210029, PR China; Jiangsu Province Institute of Materia Media, Nanjing University of Technology, Nanjing 210029, PR China.
Ning
N
Chen
School of Pharmacy, Nanjing Medical University, Nanjing 210029, PR China. Electronic address: xueminzhou001_001@hotmail.com.
Xuemin
X
Zhou
School of Pharmacy, Nanjing Medical University, Nanjing 210029, PR China. Electronic address: hongjunli1102@163.com.
Junli
J
Hong
D000327:Adsorption; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005243:Feces / Q000737:chemistry*; D052203:Ferrosoferric Oxide / Q000737:chemistry; D058185:Magnetite Nanoparticles / Q000737:chemistry; D008297:Male; D008516:Medicine, Chinese Traditional; D054802:Molecular Imprinting; D053723:Panax notoginseng / Q000737:chemistry* / Q000378:metabolism; D011108:Polymers / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D012503:Saponins / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism; D012822:Silicon Dioxide / Q000737:chemistry; D052616:Solid Phase Extraction; D012997:Solvents / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
D058185:Magnetite Nanoparticles; D011108:Polymers; D012503:Saponins; D012997:Solvents; D012822:Silicon Dioxide; D052203:Ferrosoferric Oxide
Magnetic molecularly imprinted polymers; Metabolites study; Orbitrap mass spectrometry; Panax notoginseng; Rat faeces