CRISPR/Cas9: A New Tool for RNA Imaging in Live Cells.
Cas9 can be implemented as an RNA-targeting system to track mRNA in living cells. Nuclear export is enabled by efficient targeting of GFP-fused Cas9 to an endogenous mRNA. The approach provides a new and versatile platform for RNA-targeting with applications in RNA imaging and beyond.
Institute of Biochemistry, University of Münster, Wilhelm-Klemm-Strasse 2, 48149, Münster, Germany.
Kristina
K
Rau
Institute of Biochemistry and, Cells-in-Motion Cluster of Excellence (EXC1003-CiM), University of Münster, Wilhelm-Klemm-Strasse 2, 48149, Münster, Germany. a.rentmeister@uni-muenster.de.
Andrea
A
Rentmeister
D064130:CRISPR-Associated Proteins / Q000737:chemistry / Q000378:metabolism*; D064113:CRISPR-Cas Systems; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000378:metabolism; D057054:Molecular Imaging / Q000379:methods*; D012313:RNA / Q000032:analysis* / Q000378:metabolism; D034443:RNA Transport
D064130:CRISPR-Associated Proteins; D049452:Green Fluorescent Proteins; D012313:RNA
CRISPR; Cas9; RNA; RNA imaging; RNA tracking; fluorescent probes