Anti- and Pro-apoptotic Bcl2 Proteins Distribution and Metabolic Profile in Human Coronary Aorta Endothelial Cells Before and After HypPDT.
Understanding apoptosis regulatory mechanisms in endothelial cells (ECs) has great importance for the development of novel therapy strategies for cancer and cardiovascular pathologies. An oxidative stress with the generation of reactive oxygen species (ROS) is a common mechanism causing ECs' dysfunction and apoptosis. The generation of ROS can be triggered by various stimuli including photodynamic therapy (PDT). In most PDT treatments, photosensitizer (PS) is administered systemically, and thus, possibility of high exposure to PS in the ECs remains high. PS accumulation in ECs may be clinically relevant even without PDT, if PS molecules affect the pro-apoptotic cascade without illumination. In the present work, we focused on Hypericin (Hyp) and HypPDT effects on the cell viability, oxidative stress, and the distribution of Bcl2 family members in human coronary artery endothelial (HCAEC) cells. Our findings show that the presence of Hyp itself has an effect on cell viability, oxidative stress, and the distribution of Bcl2 family members, without affecting the mitochondria function. In contrast, HypPDT resulted in mitochondria dysfunction, further increase of oxidative stress and effect on the distribution of Bcl2 family members, and in primarily necrotic type of death in HCAEC cells.
Department of Biophysics, Faculty of Natural Sciences, PJ Safarik University, Jesenna 5, Kosice, Slovakia.
Mária
M
Maslaňáková
Department of Biophysics, Faculty of Natural Sciences, PJ Safarik University, Jesenna 5, Kosice, Slovakia.
Lucia
L
Balogová
Department of Biophysics, Faculty of Natural Sciences, PJ Safarik University, Jesenna 5, Kosice, Slovakia.|Center of Interdisciplinary Biosciences, Faculty of Natural Sciences, PJ Safarik University, Kosice, Slovakia.
Pavol
P
Miškovský
Department of Respiratory Medicine, Faculty of Medicine, P.J. Safarik University, Kosice, Slovakia.
Ružena
R
Tkáčová
Department of Biophysics, Faculty of Natural Sciences, PJ Safarik University, Jesenna 5, Kosice, Slovakia. katarina.stroffekova@upjs.sk.
Katarína
K
Štroffeková
D000873:Anthracenes; D001011:Aorta / Q000166:cytology; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D055442:Metabolome / Q000187:drug effects*; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010569:Perylene / Q000031:analogs & derivatives / Q000494:pharmacology; D017319:Photosensitizing Agents / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*
D000873:Anthracenes; D051017:Apoptosis Regulatory Proteins; D017319:Photosensitizing Agents; D019253:Proto-Oncogene Proteins c-bcl-2; D017382:Reactive Oxygen Species; D010569:Perylene; C004965:hypericin
Apoptosis; Bcl2 family of proteins; Hypericin; Oxidative stress; Photodynamic therapy