Evaluation of anti-cancer activity of PLGA nanoparticles containing crocetin.
Poor solubility of crocetin has limited its use as a potential chemotherapeutic agent. In this study, a crocetin nanocarrier was formulated based on poly(lactic-co-glycolic acid) (PLGA) polymer. The effect of preparation conditions on the size and entrap efficiency of PLGA nanoparticles (NPs) via single emulsion/solvent evaporation method was evaluated. Cytotoxicity of crocetin and crocetin-encapsulated PLGA NPs was assayed in MCF-7 cell line. In PLGA formulations, the optimal condition was found to be 5% polyvinyl alcohol (PVA), crocetin:polymer ratio 1:20 and dichloromethane as organic solvent. Obtained results demonstrated that the encapsulation of crocetin in PLGA significantly increased the cytotoxicity of this compound.
a Department of Biology, School of Science , Payame Noor University , Mashhad , Iran.
Zohreh
Z
Hafezi Ghahestani
a Department of Biology, School of Science , Payame Noor University , Mashhad , Iran.
Fatemeh
F
Alebooye Langroodi
b Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences , Tabriz , Iran.|c Department of Biotechnology , Higher Education Institute of Rab-Rashid , Tabriz , Iran.
Ahad
A
Mokhtarzadeh
d Pharmaceutical Research Center, School of Pharmacy , Mashhad University of Medical Sciences , Mashhad , Iran.
Mohammad
M
Ramezani
e Nanotechnology Research Center, School of Pharmacy , Mashhad University of Medical Sciences , Mashhad , Iran.
Maryam
M
Hashemi
D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002338:Carotenoids / Q000737:chemistry* / Q000494:pharmacology*; D004337:Drug Carriers / Q000737:chemistry*; D065546:Drug Liberation; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D061986:MCF-7 Cells; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D014801:Vitamin A / Q000031:analogs & derivatives
D000970:Antineoplastic Agents; D004337:Drug Carriers; C487773:trans-sodium crocetinate; D014801:Vitamin A; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D002338:Carotenoids
Cancer; PLGA; crocetin; encapsulation; saffron