Environmental sampling protocol devised and implemented to resolve a nosocomial outbreak due to carbapenem-resistant Klebsiella oxytoca.
We describe a method for investigating wet foci in the hospital environment of a nosocomial outbreak due to carbapenem-resistant Klebsiella oxytoca. By searching in areas that are usually inaccessible, by immersing sampling swabs in thioglycolate broth, and by concentrating the sampled water, reservoirs of infection that would have gone unnoticed can be detected.
Laboratory of Microbiology, Hospital de La Merced, Osuna, Seville, Spain. Electronic address: mariac.dominguez.sspa@juntadeandalucia.es.
María Carmen
MC
Domínguez Jiménez
Internal Medicine Service, Hospital del Tomillar, Seville, Spain.
Salvador
S
Vergara-López
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001431:Bacteriological Techniques / Q000379:methods*; D015780:Carbapenems / Q000494:pharmacology; D003428:Cross Infection / Q000453:epidemiology* / Q000382:microbiology; D004196:Disease Outbreaks; D004783:Environmental Microbiology; D006801:Humans; D007710:Klebsiella Infections / Q000453:epidemiology* / Q000382:microbiology; D041121:Klebsiella oxytoca / Q000187:drug effects / Q000302:isolation & purification*; D013048:Specimen Handling / Q000379:methods*; D018440:beta-Lactam Resistance
D000900:Anti-Bacterial Agents; D015780:Carbapenems
Outbreak; Pipes