Expression Profiling of DNA Methylation and Transcriptional Repression Associated Genes in Lens Epithelium Cells of Age-Related Cataract.
In our previous research, the formation and development of age-related cataract (ARC) is associated with DNA hypermethylation of some genes in lens epithelial cells (LECs). This study aimed to investigate the expression profile of DNA methylation- and transcriptional repression-associated genes in LECs of ARC. The expression levels of the genes were first evaluated by microarray analysis. The results were further confirmed by Quantitative Real-Time PCR (qRT-PCR) and Western blot assay. The mRNA and protein levels of 5 genes increased in LECs of ARCs compared with the controls. These data provided a global perspective on expression of DNA methylation- and transcriptional repression-associated genes. The study supports the notion that the epigenetic modification of macromolecules in LECs might contribute to ARC pathogenesis.
Eye Institute, Affiliated Hospital of Nantong University, 20 Xisi Road, Jiangsu, Nantong, China.
Yong
Y
Wang
Eye Institute, Affiliated Hospital of Nantong University, 20 Xisi Road, Jiangsu, Nantong, China.
Guowei
G
Zhang
Eye Institute, Affiliated Hospital of Nantong University, 20 Xisi Road, Jiangsu, Nantong, China.
Lihua
L
Kang
Eye Institute, Affiliated Hospital of Nantong University, 20 Xisi Road, Jiangsu, Nantong, China. guanhuaijineye@163.com.
Huaijin
H
Guan
D000368:Aged; D000375:Aging / Q000235:genetics*; D016022:Case-Control Studies; D002386:Cataract / Q000235:genetics* / Q000473:pathology*; D019175:DNA Methylation / Q000235:genetics*; D004847:Epithelial Cells / Q000378:metabolism* / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007908:Lens, Crystalline / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D014158:Transcription, Genetic
D012333:RNA, Messenger
Age-related cataract; DNA methylation; Epigenetics; Lens epithelial cells; mRNA array