[Effects of PI3K/Akt Pathway in Wound Healing Process of Mice Skin].
OBJECTIVE
To investigate the expressions and time-dependent changes of phosphatidylinositol-3-kinase (PI3K), phospho-PI3K (p-PI3K), protein kinase B (PKB/Akt) and phospho-Akt (p-Akt) during wound healing process of mice skin.


METHODS
The changes of PI3K, p-PI3K, Akt and p-Akt expression in skin wound were detected by immunohistochemistry, Western blotting and real-time PCR.


RESULTS
Immunohistochemistry showed the expression of PI3K and p-Akt were observed in mononuclear and fibroblast after skin wound, and reached peak in reconstruction. The positive bands of PI3K, p-PI3K, Akt and p-Akt were observed in all time points of the wound healing process by Western blotting. The expression peak of p-PI3K and p-Akt showed in inflammation and proliferation; the expression peak of PI3K and Akt in reconstruction. Real-time PCR showed the expression peak of PI3K mRNA in inflammation and reconstruction and the peak of Akt mRNA in reconstruction.


CONCLUSIONS
During the wound healing process, the expressions of PI3K, Akt, p-PI3K and p-Akt show different changes with significant correlation to wound time. The expression of PI3K/Akt may be a valuable marker for wound time estimation.
Yan-ling
YL
Gao
Chang-sheng
CS
Liu
Rui
R
Zhao
Lin-lin
LL
Wang
Shan-shan
SS
Li
Min
M
Liu
Miao
M
Zhang
Shu-kun
SK
Jiang
Zhi-ling
ZL
Tian
Meng
M
Wang
Da-wei
DW
Guan
D000818:Animals; D015153:Blotting, Western; D058534:Class I Phosphatidylinositol 3-Kinases; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D058539:Phosphatidylinositol 3-Kinase; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics* / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction; D012867:Skin / Q000201:enzymology / Q000293:injuries*; D014945:Wound Healing
D058534:Class I Phosphatidylinositol 3-Kinases; D058539:Phosphatidylinositol 3-Kinase; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; C421683:Pik3cd protein, mouse