Gene expression and enzyme activity of lipoprotein lipase correlate with intramuscular fat content in Guangxi san-huang and Arbor Acres chickens.
Lipoprotein lipase (LPL) is a key enzyme in lipid metabolism. This study investigated LPL gene expression, LPL enzyme activity, and the correlation of each with intramuscular fat (IMF) in Chinese Guangxi san-huang (GXSH) and Arbor Acres (AA) chickens. The results showed that age and breed had significant effects on LPL expression and enzyme activity. Correlation analyses showed significant positive correlations between LPL expression levels and IMF contents in the breast and thigh tissues of both GXSH (r = 0.712, P = 0.001; r = 0.792, P < 0.001, respectively) and AA (r = 0.644, P < 0.001; r = 0.545, P < 0.001, respectively) chickens. The results also indicated a significant positive correlation between LPL enzyme activity and IMF contents in the breast and thigh tissues of both GXSH (r = 0.615, P = 0.001; r = 0.685, P < 0.001, respectively) and AA (r = 0.600, P = 0.001; r = 0.528, P = 0.003, respectively) chickens. The results indicated that the LPL gene was significantly correlated with IMF in these two breeds. The results presented here could contribute to knowledge of LPL mRNA developmental expression patterns and enzyme activity, and it could facilitate further research on the molecular mechanisms underlying IMF deposition in chickens.
College of Animal Science and Technology, Guangxi University, Nanning, China.
Y N
YN
Huang
College of Animal Science and Technology, Guangxi University, Nanning, China.
J
J
Wang
College of Animal Science and Technology, Guangxi University, Nanning, China.
B J
BJ
Chen
College of Animal Science and Technology, Guangxi University, Nanning, China.
Q Y
QY
Jiang
College of Animal Science and Technology, Guangxi University, Nanning, China.
Y F
YF
Guo
College of Animal Science and Technology, Guangxi University, Nanning, China.
G Q
GQ
Lan
College of Animal Science and Technology, Guangxi University, Nanning, China.
H S
HS
Jiang
D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D002645:Chickens / Q000235:genetics*; D008071:Lipoprotein Lipase / Q000235:genetics* / Q000378:metabolism; D008460:Meat / Q000592:standards*; D018482:Muscle, Skeletal / Q000378:metabolism
D008071:Lipoprotein Lipase