Olive Leaf Extract from Sicilian Cultivar Reduced Lipid Accumulation by Inducing Thermogenic Pathway during Adipogenesis.
Olive leaves contain a wide variety of phenolic compounds belonging to phenolic acids, phenolic alcohols, flavonoids, and secoiridoids, and include also many other pharmacological active compounds. They could play an important role in human diet and health because of their ability to lower blood pressure, increase coronary arteries blood flow and decrease the risk of cardiovascular diseases. The aim of this study was to investigate the effect of olive leaf extract (OLE) from Sicilian cultivar on adipogenic differentiation of human adipose derived mesenchymal stem cells and its impact on lipid metabolism. We showed that OLE treatment during adipogenic differentiation reduces inflammation, lipid accumulation and induces thermogenesis by activation of uncoupling protein uncoupling protein 1, sirtuin 1, peroxisome proliferator-activated receptor alpha, and coactivator 1 alpha. Furthermore, OLE significantly decreases the expression of molecules involved in adipogenesis and upregulates the expression of mediators involved in thermogenesis and lipid metabolism. Taken together, our results suggest that OLE may promote the brown remodeling of white adipose tissue inducing thermogenesis and improving metabolic homeostasis.
Department of Agricultural, Food and Environment, University of Catania Catania, Italy.
Rosa
R
Palmeri
Department of Agricultural, Food and Environment, University of Catania Catania, Italy.
Julieta I
JI
Monteleone
Department of Agricultural, Food and Environment, University of Catania Catania, Italy.
Giovanni
G
Spagna
Department of Agricultural, Food and Environment, University of Catania Catania, Italy.
Cristina
C
Restuccia
Biochemistry Section, Department of Drug Sciences, University of Catania Catania, Italy.
Marco
M
Raffaele
Biochemistry Section, Department of Drug Sciences, University of Catania Catania, Italy.
Luca
L
Vanella
Department of Biomedical and Biotechnological Sciences, University of CataniaCatania, Italy; Euro-Mediterranean Institute of Science and TechnologyPalermo, Italy.
Giovanni
G
Li Volti
Biochemistry Section, Department of Drug Sciences, University of CataniaCatania, Italy; Euro-Mediterranean Institute of Science and TechnologyPalermo, Italy.
Ignazio
I
Barbagallo
adipocyte; heme oxygenase; lipid metabolism; olive leaf extract; stem cells differentiation; thermogenesis