Glycerol Production from Methanol by a Respiration-deficient Mutant Strain of a Methylotrophic Yeast, Candida boidinii No. 2201.
A mutant strain of a methylotrophic yeast, Candida boidinii UV-16, which was obtained and characterized as a respiration-deficient mutant, showed an improved productivity of glycerol from methanol. Cultural conditions for glycerol production was studied. Feedings of methanol and a nitrogen source during cultivation promoted the glycerol production by strain UV-16. Biotin and thiamin exclusion from the culture medium repressed the growth but doubled the glycerol production. The presence of slight amounts of the vitamins from seed culture was necessary and stimulative for the glycerol production. Addition of antifoam stimulated the ascent of glycerol productivity. Under the best culture conditions, strain UV-16 produced 2.18g/liters of glycerol in a methanol medium.
a Department of Agricultural Chemistry , Kyoto University , Kyoto 606-01, Japan.
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Yamada
a Department of Agricultural Chemistry , Kyoto University , Kyoto 606-01, Japan.
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Yoshieda
a Department of Agricultural Chemistry , Kyoto University , Kyoto 606-01, Japan.
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Hori
a Department of Agricultural Chemistry , Kyoto University , Kyoto 606-01, Japan.
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Tani