Punicalagin alleviates hepatotoxicity in rats challenged with cyclophosphamide.
This study investigated the possible hepatoprotection of punicalagin in rats received cyclophosphamide (20mg/kg/day, i.p., for 7 days). Punicalagin given at two doses, 15 and 30mg/kg/day, p.o., for 7 days, starting the same day of cyclophosphamide administration. Punicalagin significantly and dose-dependently reduced the elevations of serum alanine aminotransferase, and liver nuclear factor-κB p65, tumor necrosis factor-α, interleukin-1β, malondialdehyde, nitric oxide, Bax/Bcl-2 ratio, inducible nitric oxide synthase, caspases 3 and 9 activities, and prevented the decrease of hepatic total antioxidant capacity. Punicalagin also attenuated the histopathological liver tissue damage, and decreased cyclooxygenase-2 expression in liver of rats received cyclophosphamide in a dose-dependent manner. It was concluded that punicalagin protected rat liver against cyclophosphamide toxicity by inhibiting oxidative/nitrosative stress, inflammation, and apoptosis.
Department of Biomedical Sciences, Pharmacology Division, College of Medicine, King Faisal University, Al-Ahsa, Saudi Arabia. Electronic address: amrfouad65@yahoo.com.
Amr A
AA
Fouad
Department of Internal Medicine, College of Medicine, King Faisal University, Al-Ahsa, Saudi Arabia.
Hatem O
HO
Qutub
Department of Biomedical Sciences, Histopathology Division, College of Medicine, King Faisal University, Al-Ahsa, Saudi Arabia.
Walid N
WN
Al-Melhim
D000284:Administration, Oral; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D015415:Biomarkers / Q000097:blood; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D003520:Cyclophosphamide / Q000008:administration & dosage / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D047348:Hydrolyzable Tannins / Q000008:administration & dosage / Q000627:therapeutic use*; D008111:Liver Function Tests; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D020011:Protective Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D017207:Rats, Sprague-Dawley
D015415:Biomarkers; D047348:Hydrolyzable Tannins; D020011:Protective Agents; C115642:punicalagin; D003520:Cyclophosphamide
Cyclophosphamide; Liver; Punicalagin; Rats