Cytoarchitecture, Proliferative Activity and Neuroblast Migration in the Subventricular Zone and Lateral Ventricle Extension of the Adult Guinea Pig Brain.
In the mouse brain, neuroblasts generated in the subventricular zone (SVZ) migrate to the olfactory bulb (OB) through the rostral migratory stream (RMS). Although the RMS is not present in the human brain, a migratory pathway that is organized around a ventricular cavity that reaches the OB has been reported. A similar cavity, the lateral ventricle extension (LVE), is found in the adult guinea pig brain. Therefore, we analyzed cytoarchitecture, proliferative activity and precursor cell migration in the SVZ and LVE of 1-, 6- and 12-month-old guinea pigs. In young animals, we used confocal spectral and transmission electron microscopy to identify neuroblasts, astrocytes, and progenitor cells in the SVZ and LVE. Analysis of peroxidase diffusion demonstrated that the LVE was a continuous cavity lined by ependymal cells and surrounded by neuroblasts. Precursor cells were mostly located in the SVZ and migrated from the SVZ to the OB through the LVE. Finally, analysis of 6- and 12-month-old guinea pigs revealed that the LVE was preserved in older animals; however, the number of neurogenic cells was significantly reduced. Consequently, we propose that the guinea pig brain may be used as a new neurogenic model with increased similarity to humans, given that the LVE connects the LV with the OB, as has been described in humans, and that the LVE works a migratory pathway. Stem Cells 2016;34:2574-2586.
Departamento De Biología Celular, Laboratorio De Neurobiología Y Células Madres, Centro De Microscopía Avanzada CMA-BIOBIO, Facultad De Ciencias Biológicas, Universidad De Concepción, Concepción, Chile.
Nery
N
Jara
Departamento De Biología Celular, Génetica Y Fisiología, Laboratorio De Fisiología Animal, Facultad De Ciencias, Centro De Investigaciones Biomédicas En Red De Bioingeniería, Biomateriales Y Nanomedicina (CIBER-BBN), Universidad De Málaga, Málaga, España. mcifuentes@uma.es.
Manuel
M
Cifuentes
Departamento De Biología Celular, Laboratorio De Neurobiología Y Células Madres, Centro De Microscopía Avanzada CMA-BIOBIO, Facultad De Ciencias Biológicas, Universidad De Concepción, Concepción, Chile.
Fernando
F
Martínez
Departamento De Biología Celular, Laboratorio De Neurobiología Y Células Madres, Centro De Microscopía Avanzada CMA-BIOBIO, Facultad De Ciencias Biológicas, Universidad De Concepción, Concepción, Chile.
Katterine
K
Salazar
Departamento De Biología Celular, Laboratorio De Neurobiología Y Células Madres, Centro De Microscopía Avanzada CMA-BIOBIO, Facultad De Ciencias Biológicas, Universidad De Concepción, Concepción, Chile. frnualart@udec.cl.
Francisco
F
Nualart
D000375:Aging / Q000502:physiology*; D000818:Animals; D002465:Cell Movement; D049109:Cell Proliferation; D048430:Cell Shape; D006168:Guinea Pigs; D020547:Lateral Ventricles / Q000166:cytology* / Q000648:ultrastructure; D008297:Male; D009474:Neurons / Q000166:cytology*
Adult neurogenesis; Lateral ventricle extension; Neural stem cells; Subventricular zone