Enhancement of Matrix Metalloproteinase-2 (MMP-2) as a Potential Chondrogenic Marker during Chondrogenic Differentiation of Human Adipose-Derived Stem Cells.
Human adipose-derived stem cells (hASCs) have a capacity to undergo adipogenic, chondrogenic, and osteogenic differentiation. Recently, hASCs were applied to various fields including cell therapy for tissue regeneration. However, it is hard to predict the direction of differentiation of hASCs in real-time. Matrix metalloproteinases (MMPs) are one family of proteolytic enzymes that plays a pivotal role in regulating the biology of stem cells. MMPs secreted by hASCs are expected to show different expression patterns depending on the differentiation state of hASCs because biological functions exhibit different patterns during the differentiation of stem cells. Here, we investigated proteolytic enzyme activity, especially MMP-2 activity, in hASCs during their differentiation. The activities of proteolytic enzymes and MMP-2 were higher during chondrogenic differentiation than during adipogenic and osteogenic differentiation. During chondrogenic differentiation, mRNA expression of MMP-2 and the level of the active form of MMP-2 were increased, which also correlated with Col II. It is concluded that proteolytic enzyme activity and the level of the active form of MMP-2 were increased during chondrogenic differentiation, which was accelerated in the presence of Col II protein. According to our findings, MMP-2 could be a candidate maker for real-time detection of chondrogenic differentiation of hASCs.
Department of Biomedical Science, CHA University, Seongnam-si, Gyeonggi-do 443-742, Korea. ruddov@gmail.com.
Yoshie
Y
Arai
Department of Biomedical Science, CHA University, Seongnam-si, Gyeonggi-do 443-742, Korea. nightsky9836@naver.com.
Sunghyun
S
Park
Department of Biomedical Science, CHA University, Seongnam-si, Gyeonggi-do 443-742, Korea. bgchoi725@gmail.com.
Bogyu
B
Choi
Department of Biomedical Science, CHA University, Seongnam-si, Gyeonggi-do 443-742, Korea. glintk@naver.com.
Kyoung-Won
KW
Ko
Department of Orthopedic Surgery, Bundang Medical Center, CHA University, Seongnam-si, Gyeonggi-do 443-742, Korea. wcosdoc@gmail.com.
Won Chul
WC
Choi
Department of Orthopedic Surgery, Bundang Medical Center, CHA University, Seongnam-si, Gyeonggi-do 443-742, Korea. drjmlee@naver.com.
Joong-Myung
JM
Lee
Department of Optics and Mechatronics Engineering, BK21+ Nano-Integrated Cogno-Mechatronics Engineering, College of Nanoscience &amp; Nanotechnology, Pusan National University, Busan 619-961, Korea. nanohan@pusan.ac.kr.
Dong-Wook
DW
Han
Department of Biomedical Engineering, Collage of Medicine, Kyung Hee University, Seoul 151-742, Korea. sigmoidus@khu.ac.kr.
Hun-Kuk
HK
Park
Department of Neurosurgery, Bundang Medical Center, CHA University, Seongnam-si, Gyeonggi-do 443-742, Korea. hanib@cha.ac.kr.
Inbo
I
Han
Department of Food Science and Biotechnology, College of Life Science, CHA University, Gyeonggi-do 443-742, Korea. mushroom14@gmail.com.
Jong Hun
JH
Lee
Department of Biomedical Science, CHA University, Seongnam-si, Gyeonggi-do 443-742, Korea. soohong@cha.ac.kr.
Soo-Hong
SH
Lee
D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*
D015415:Biomarkers; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2
chondrogenic differentiation; extracellular matrix; human adipose-derived stem cells; matrix metalloproteinase-2