Fractionation and Antitumor Activity of Polysaccharides from Grifola frondosa Mycelium.
We developed a method for the fractionation and purification of antitumor polysaccharides, considered to be a type of immuno-potentiator or BRM (biological response modifier), from the mycelium of liquid cultured Grifola frondosa. The active polysaccharide fractions that showed higher antitumor activity were considered to be heteroglycans or their protein complexes as follows, in water-soluble fractions: FI0-a-α: fucoga-lactomannan-protein complex; FI0-a-β: mannogalactofucan; FA-1: galactoglucomannofucan-protein complex; FA-2-b-α: glucogalactomannan-protein complex; in water-insoluble fractions: FIII-1-a: mannofucoglucoxylan; FIII-1-b: mannoglucofucoxylan-protein complex; FIII-2-a: mannofucoglucoxylan-protein complex; FIII-2-b: glucomannofucoxylan-protein complex.
a The United Graduate School of Agricultural Sciences, Gifu University (Shizuoka University) , 1-1 Yanagido, Gifu 501-11 , Japan.
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Zhuang
a The United Graduate School of Agricultural Sciences, Gifu University (Shizuoka University) , 1-1 Yanagido, Gifu 501-11 , Japan.|b Faculty of Agriculture, Shizuoka University , Ohya, Shizuoka 422 , Japan.
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Mizuno
a The United Graduate School of Agricultural Sciences, Gifu University (Shizuoka University) , 1-1 Yanagido, Gifu 501-11 , Japan.|c School of Medicine, Mie University , Tsu, Mie 514 , Japan.
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Ito
a The United Graduate School of Agricultural Sciences, Gifu University (Shizuoka University) , 1-1 Yanagido, Gifu 501-11 , Japan.|c School of Medicine, Mie University , Tsu, Mie 514 , Japan.
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Shimura
a The United Graduate School of Agricultural Sciences, Gifu University (Shizuoka University) , 1-1 Yanagido, Gifu 501-11 , Japan.|d Iwade Mushroom Institute , Tsu, Mie 514 , Japan.
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Sumiya
a The United Graduate School of Agricultural Sciences, Gifu University (Shizuoka University) , 1-1 Yanagido, Gifu 501-11 , Japan.|d Iwade Mushroom Institute , Tsu, Mie 514 , Japan.
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Kawade