Salivary Metabolite Fingerprint of Type 1 Diabetes in Young Children.
Metabolomics is an important tool for the evaluation of the human condition, in both health or disease. This study analyzed the salivary components of type I diabetic children (DM1) under six years of age, to assess oral health related to diabetes control, as well as metabolite profiling using NMR. Partial least squared discriminant analysis (PLS-DA) was used to compare healthy (HG) and uncontrolled DM1 subjects that demonstrated a separation between the groups with classificatory performance of ACC = 0.80, R(2) = 0.92, Q(2) = 0.02 and for DM1 children with glycemia >200 mg/dL of ACC = 0.74, R(2) = 0.91, Q(2) = 0.06. The metabolites that mostly contributed to the distinction between the groups in the loading factor were acetate, n-acetyl-sugar, lactate, and sugar. The univariate analysis showed a decreased salivary concentration of succinic acid and increased levels of lactate, acetate, and sucrose in uncontrolled and DM1 children with glycemia >200 mg/dL. The present study demonstrates that the salivary profile of DM1 differs from that of HG children. It appears that diabetes status control has an important effect on the salivary composition.
Department of Pediatric Dentistry and Orthodontics, School of Dentistry, Federal University of Rio de Janeiro , 21941-971 Rio de Janeiro, RJ, Brazil.
Livia Roberta Piedade
LR
de Oliveira
Department of Pediatric Dentistry and Orthodontics, School of Dentistry, Federal University of Rio de Janeiro , 21941-971 Rio de Janeiro, RJ, Brazil.
Carla
C
Martins
Department of Pediatric Dentistry and Orthodontics, School of Dentistry, Federal University of Rio de Janeiro , 21941-971 Rio de Janeiro, RJ, Brazil.
Tatiana Kelly Silva
TK
Fidalgo
Department of Pediatric Dentistry and Orthodontics, School of Dentistry, Federal University of Rio de Janeiro , 21941-971 Rio de Janeiro, RJ, Brazil.
Liana Bastos
LB
Freitas-Fernandes
Department of Pediatric Dentistry and Orthodontics, School of Dentistry, Federal University of Rio de Janeiro , 21941-971 Rio de Janeiro, RJ, Brazil.
Rafaela
R
de Oliveira Torres
Department of Pediatric Dentistry and Orthodontics, School of Dentistry, Federal University of Rio de Janeiro , 21941-971 Rio de Janeiro, RJ, Brazil.
Aline Laignier
AL
Soares
National Center for Nuclear Magnetic Resonance - Jiri Jonas, Medical Biochemistry Institute, Federal University of Rio de Janeiro , 21.941-590 Rio de Janeiro, RJ, Brazil.
Fabio C L
FC
Almeida
National Center for Nuclear Magnetic Resonance - Jiri Jonas, Medical Biochemistry Institute, Federal University of Rio de Janeiro , 21.941-590 Rio de Janeiro, RJ, Brazil.
Ana Paula
AP
Valente
Department of Pediatric Dentistry and Orthodontics, School of Dentistry, Federal University of Rio de Janeiro , 21941-971 Rio de Janeiro, RJ, Brazil.
Ivete Pomarico Ribeiro
IP
de Souza
D000085:Acetates / Q000378:metabolism; D016022:Case-Control Studies; D002675:Child, Preschool; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism*; D016002:Discriminant Analysis; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D055432:Metabolomics; D012463:Saliva / Q000737:chemistry / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism
D000085:Acetates; D019344:Lactic Acid; D013395:Sucrose
NMR; children; metabolomics; saliva; type 1 diabetes mellitus