[Roles of CYP2E1 in liver damage induced by 1,2-dichloroethane].
OBJECTIVE
To explore the expression of CYP2E1 in the liver of mice and its effects on liver injury induced by 1,2-dichloroethane (1, 2-DCE).


METHODS
(1) Thirty-two female mice were randomly divided into four groups, which were control group, low dose group, medium dose group and high dose group. Mice were exposed to 1,2-DCE through respiratory for 4 h per day for 10 days. At the end of exposure, the mice were sacrificed, their blood and liver were collected rapidly. Pathological analysis was examined. Activity of ALT and AST in serum and activity of CYP2E1 in liver were mice were randomly divided into six groups, ie simple control group, corn oil control group, inhibitor control group, simple poisonous group, low and high dose diallyl sulfide (DAS) intervention groups. Mice were treated orally with DAS in corn oil 4 hours before 1, 2-DCE exposure. The examination indicators were as aforementioned.


RESULTS
(1) Compared to control group, activity of ALT in serum of mice in the high dose group and expression of CYP2E1 in the liver of mice in medium and high dose group increased significantly. In addition, the histological observation suggested obvious liver damage in medium and high dose group. (2) CYP2E1 protein expression and activity in liver and ALT in serum decreased significantly in DAS-intervention groups compared to simple poisonous group. Along with the changes of CYP2E1 and ALT, pathological changes of liver damage was better.


CONCLUSIONS
Our results suggested that expression of CYP2E1 and oxidative damage in the liver could be induced by 1,2-DCE exposure, and CYP2E1 inhibitors can reduce the hepatic tissue damage caused by DCE.
Guangqian
G
Yang
Lei
L
Shi
Jun
J
Zhang
Xiaoqiong
X
Tan
Gaoyang
G
Wang
Xiuqiang
X
Lv
Yaping
Y
Jin
D000410:Alanine Transaminase / Q000378:metabolism; D000498:Allyl Compounds / Q000494:pharmacology*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000201:enzymology*; D019392:Cytochrome P-450 CYP2E1 / Q000378:metabolism*; D005025:Ethylene Dichlorides / Q000633:toxicity*; D005260:Female; D019570:Inhalation Exposure; D051379:Mice; D013440:Sulfides / Q000494:pharmacology*
D000498:Allyl Compounds; D005025:Ethylene Dichlorides; D013440:Sulfides; C024565:ethylene dichloride; C038491:allyl sulfide; D019392:Cytochrome P-450 CYP2E1; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase