Significant correlation of angiotensin converting enzyme and glycoprotein IIIa genes polymorphisms with unexplained recurrent pregnancy loss in north of Iran.
BACKGROUND
Spontaneous abortion is considered as the most complex problem during pregnancy. Thrombophilia is resumed as a cause of recurrent pregnancy loss (RPL). Glycoprotein IIIa (GPIIIa) gene is involved in thrombosis and abortion. Angiotensin converting enzyme (ACE) converts angiotensin I to angiotensin II and is involved in thrombosis. The most common polymorphism in this gene is the insertion/deletion (I/D).


OBJECTIVE
In this study, we analyzed the association between ACE I/D and GPIIIa c.98C >T polymorphisms in women with unexplained RPL from the north of Iran.


METHODS
Sample population consisted of 100 women with unexplained RPL and 100 controls. The ACE I/D and GPIIIa c.98C>T polymorphisms were genotyped by TETRA-ARMS PCR. The association between genotypes frequency and RPL were analyzed using χ(2) and exact fisher tests. Associated risk with double genotype combinations was also investigated by binary logistic regression.


RESULTS
There was significant association between ACE DD genotype and RPL (OR=2.04; 95% CI=0.94-4.44; p=0.036). ACE D Allele was also significantly associated with the RPL (OR=1.59; 95% CI=1.05-2.41; p=0.013). No significant association was observed between GPIIIa c.98C>T polymorphism and RPL.


CONCLUSIONS
ACE I/D polymorphism may probably be a prognostic factor in female family members of women with the history of recurrent abortion.
Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran.
Shokoufeh
S
Fazelnia
Medical Cellular and Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
Touraj
T
Farazmandfar
Immunogenetic Research center, Molecular and cell Biology Research Centre, Mazandaran University of Medical Sciences, Sari, Iran.
Seyed Mohammad Bagher
SM
Hashemi-Soteh
Angiotensin converting enzyme; Platelet glycoprotein IIIa; Recurrent abortion