Gold Nanoparticles Decorated with Sialic Acid Terminated Bi-antennary N-Glycans for the Detection of Influenza Virus at Nanomolar Concentrations.
Gold nanoparticles decorated with full-length sialic acid terminated complex bi-antennary N-glycans, synthesized with glycans isolated from egg yolk, were used as a sensor for the detection of both recombinant hemagglutinin (HA) and whole influenza A virus particles of the H1N1 subtype. Nanoparticle aggregation was induced by interaction between the sialic acid termini of the glycans attached to gold and the multivalent sialic acid binding sites of HA. Both dynamic light scattering (DLS) and UV/Vis spectroscopy demonstrated the efficiency of the sensor, which could detect viral HA at nanomolar concentrations and revealed a linear relationship between the extent of nanoparticle aggregation and the concentration of HA. UV/Vis studies also showed that these nanoparticles can selectively detect an influenza A virus strain that preferentially binds sialic acid terminated glycans with α(2→6) linkages over a strain that prefers glycans with terminal α(2→3)-linked sialic acids.
Department of Chemistry University of Canterbury Private Bag 4800 Christchurch 8140 New Zealand; The MacDiarmid Institute for Advanced Materials and Nanotechnology Wellington 6140 New Zealand.
Vivek
V
Poonthiyil
Department of Microbiology and Immunology University of Otago PO Box 56 Dunedin 9054 New Zealand.
Prashanth T
PT
Nagesh
Department of Microbiology and Immunology University of Otago PO Box 56 Dunedin 9054 New Zealand.
Matloob
M
Husain
Department of Chemistry University of Canterbury Private Bag 4800 Christchurch 8140 New Zealand; The MacDiarmid Institute for Advanced Materials and Nanotechnology Wellington 6140 New Zealand.
Vladimir B
VB
Golovko
Department of Chemistry University of Canterbury Private Bag 4800 Christchurch 8140 New Zealand; Biomolecular Interaction Centre University of Canterbury Private Bag 4800 Christchurch 8140 New Zealand.
Antony J
AJ
Fairbanks
carbohydrates; gold nanoparticles; hemagglutinin; influenza; sensors; sialylglycan