Establishment of cell surface engineering and its development.
Cell surface display of proteins/peptides has been established based on mechanisms of localizing proteins to the cell surface. In contrast to conventional intracellular and extracellular (secretion) expression systems, this method, generally called an arming technology, is particularly effective when using yeasts as a host, because the control of protein folding that is often required for the preparation of proteins can be natural. This technology can be employed for basic and applied research purposes. In this review, I describe various strategies for the construction of engineered yeasts and provide an outline of the diverse applications of this technology to industrial processes such as the production of biofuels and chemicals, as well as bioremediation and health-related processes. Furthermore, this technology is suitable for novel protein engineering and directed evolution through high-throughput screening, because proteins/peptides displayed on the cell surface can be directly analyzed using intact cells without concentration and purification. Functional proteins/peptides with improved or novel functions can be created using this beneficial, powerful, and promising technique.
a Division of Applied Life Sciences, Graduate School of Agriculture , Kyoto University , Sakyo-ku , Japan.
Mitsuyoshi
M
Ueda
D001673:Biodegradation, Environmental; D056804:Biofuels; D060846:Cell Engineering / Q000379:methods* / Q000639:trends; D015966:Gene Expression Regulation, Fungal; D057166:High-Throughput Screening Assays; D000072235:Mating Factor / Q000235:genetics / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008666:Metalloendopeptidases / Q000235:genetics / Q000378:metabolism; D019151:Peptide Library; D017510:Protein Folding; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism
D056804:Biofuels; D008565:Membrane Proteins; D019151:Peptide Library; D000072235:Mating Factor; D020782:Matrix Metalloproteinases; D008666:Metalloendopeptidases; C062316:neurolysin
arming technology; cell surface engineering; engineered yeast; protein engineering; whole-cell biocatalyst