Unique Vibrational Features as a Direct Probe of Specific Antigen-Antibody Recognition at the Surface of a Solid-Supported Hybrid Lipid Bilayer.
Here, we demonstrate how sum frequency generation (SFG), a vibrational spectroscopy based on a nonlinear three-photon mixing process, may provide a direct and unique fingerprint of bio-recognition; This latter can be detected with an intrinsically discriminating unspecific adsorption, thanks to the high sensitivity of the second-order nonlinear optical (NLO) response to preferential molecular orientation and symmetry properties. As a proof of concept, we have detected the biological event at the solid/liquid interface of a model bio-active antigen platform, based on a solid-supported hybrid lipid bilayer (ss-HLB) of a 2,4-dinitrophenyl (DNP) lipid, towards a monoclonal mouse anti-DNP complementary antibody.
Research Centre in Physics of Matter and Radiation (PMR), Namur Research Institute for LIfe Sciences (NARILIS), University of Namur (UNamur), 61 rue de Bruxelles, 5000, Namur, Belgium.
Dan
D
Lis
Research Centre in Physics of Matter and Radiation (PMR), Namur Research Institute for LIfe Sciences (NARILIS), University of Namur (UNamur), 61 rue de Bruxelles, 5000, Namur, Belgium. francesca.cecchet@unamur.be.
Francesca
http://orcid.org/0000-0001-7740-3383
F
Cecchet
D000818:Animals; D000936:Antigen-Antibody Complex / Q000737:chemistry*; D008051:Lipid Bilayers / Q000138:chemical synthesis / Q000737:chemistry*; D051379:Mice; D015335:Molecular Probes / Q000737:chemistry*; D013057:Spectrum Analysis; D013499:Surface Properties; D014732:Vibration
D000936:Antigen-Antibody Complex; D008051:Lipid Bilayers; D015335:Molecular Probes
antigen-antibody recognition; bio-detection; lipid bilayers; sum frequency generation; vibrational spectroscopy