Insecticidal and Enzyme Inhibitory Activities of Sparassol and Its Analogues against Drosophila suzukii.
Drosophila suzukii is an economically important pest in America and Europe as well as in Asia. Sparassol and methyl orsellinate are naturally produced by the cultivating mushrooms Sparassis cripta and Sparassis latifolia. Fumigant and contact toxicities of synthetic sparassol and its analogues, methyl orsellinate and methyl 2,4-dimethoxy-6-methylbenzoate (DMB), were investigated. Negligible fumigant activity was observed from the tested compounds. However, DMB showed the strongest contact toxicity, followed by sparassol and methyl orsellinate. The possible modes of action of the compounds were assessed for their acetylcholinesterase (AChE)- and glutathione S-transferase (GST)-inhibiting activities. AChE activity was weakly inhibited by methyl orsellinate and DMB, but GST was inhibited by sparassol, methyl orsellinate, and DMB. Thus, DMB could be a promising alternative to common insecticides as it can be easily synthesized from sparassol, which is the natural product of Sparassis species. Sparassis species could be an industrial resource of DMB.
Junheon
J
Kim
Miyeon
M
Jang
Southern Forest Resources Research Center, National Institute of Forest Science , Jinju 52817, Republic of Korea.
Kyoung-Tae
KT
Lee
Department of Agricultural Biotechnology, Seoul National University , Seoul 08826, Republic of Korea.
Kyungjae Andrew
KA
Yoon
Chung Gyoo
CG
Park
D000110:Acetylcholinesterase / Q000378:metabolism; D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D004330:Drosophila / Q000187:drug effects* / Q000201:enzymology; D029721:Drosophila Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D005982:Glutathione Transferase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D007300:Insect Control; D007306:Insecticides / Q000737:chemistry / Q000494:pharmacology*; D012118:Resorcinols / Q000737:chemistry / Q000494:pharmacology*; D012621:Seasons
D029721:Drosophila Proteins; D004791:Enzyme Inhibitors; D007306:Insecticides; D012118:Resorcinols; C472106:methyl orsellinate; D005982:Glutathione Transferase; D000110:Acetylcholinesterase
Asian vinegar fly; methyl 2,4-dimethoxy-6-methylbenzoate; methyl orsellinate; sparassol; spotted wing drosophila