Expression pattern and pharmacological characterisation of two novel alternative splice variants of the glutamate-gated chloride channel in the small brown planthopper Laodelphax striatellus.
BACKGROUND
Glutamate-gated chloride channels (GluCl) mediate fast inhibitory neurotransmission in invertebrate nervous systems. Although only one GluCl gene was presented in insects, it showed diverse alternative splicing that was speculated could affect channel function and pharmacology.


RESULTS
In this study, we isolated GluCl cDNAs from adults of the small brown planthopper (SBPH) Laodelphax striatellus and showed that six L. striatellus GluCl variants (LsGluCl-AS, LsGluCl-BS, LsGluCl-CS, LsGluCl-AL, LsGluCl-BL, LsGluCl-CL) were present in the SBPH. The expression patterns of six variants differed among developmental stages (egg, first- to fifth-instar nymphs, male and female adults) and among the body parts (head, thorax, abdomen, leg) of the female adult SBPH. All the transcripts were abundant in the head of the adult. When expressed in African clawed frog, Xenopus laevis, oocytes, the two functional variants (LsGluCl-AS, LsGluCl-AL) had similar EC50and IC50values for L-glutamate and channel blockers picrotoxinin and fipronil.


CONCLUSIONS
This study represents a comprehensive molecular, expression and pharmacological characterisation of GluCl in the SBPH. These findings should be useful in providing more opportunities to discover novel insect control chemicals. © 2016 Society of Chemical Industry.
College of Plant Protection, Nanjing Agricultural University, Nanjing, China.|State and Local Joint Engineering Research Centre of Green Pesticide Invention and Application, Jiangsu, China.
Shun-Fan
SF
Wu
College of Plant Protection, Nanjing Agricultural University, Nanjing, China.|State and Local Joint Engineering Research Centre of Green Pesticide Invention and Application, Jiangsu, China.
Xi-Chao
XC
Mu
College of Plant Protection, Nanjing Agricultural University, Nanjing, China.|State and Local Joint Engineering Research Centre of Green Pesticide Invention and Application, Jiangsu, China.
Yao-Xue
YX
Dong
College of Plant Protection, Nanjing Agricultural University, Nanjing, China.|State and Local Joint Engineering Research Centre of Green Pesticide Invention and Application, Jiangsu, China.
Li-Xiang
LX
Wang
College of Plant Protection, Nanjing Agricultural University, Nanjing, China.|State and Local Joint Engineering Research Centre of Green Pesticide Invention and Application, Jiangsu, China.
Qi
Q
Wei
College of Plant Protection, Nanjing Agricultural University, Nanjing, China.|State and Local Joint Engineering Research Centre of Green Pesticide Invention and Application, Jiangsu, China.
Cong-Fen
CF
Gao
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018118:Chloride Channels / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D005260:Female; D058787:GABA-A Receptor Antagonists / Q000494:pharmacology*; D018698:Glutamic Acid / Q000378:metabolism; D006430:Hemiptera / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D007306:Insecticides / Q000494:pharmacology*; D007928:Lethal Dose 50; D008297:Male; D009758:Nymph / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D010852:Picrotoxin / Q000031:analogs & derivatives / Q000494:pharmacology; D011720:Pyrazoles / Q000494:pharmacology; D016415:Sequence Alignment; D054830:Sesterterpenes
D018118:Chloride Channels; D018076:DNA, Complementary; D058787:GABA-A Receptor Antagonists; D018698:Glutamic Acid; D019476:Insect Proteins; D007306:Insecticides; D010852:Picrotoxin; D011720:Pyrazoles; D054830:Sesterterpenes; C109777:glutamate-gated chloride channels; C022961:picrotoxinin; C082360:fipronil
alternative splicing; fipronil; glutamate-gated chloride channel; ivermectin; small brown planthopper