Acute alcohol exposure during mouse gastrulation alters lipid metabolism in placental and heart development: Folate prevention.
BACKGROUND
Embryonic acute exposure to ethanol (EtOH), lithium, and homocysteine (HCy) induces cardiac defects at the time of exposure; folic acid (FA) supplementation protects normal cardiogenesis (Han et al., , ; Serrano et al., ). Our hypothesis is that EtOH exposure and FA protection relate to lipid and FA metabolism during mouse cardiogenesis and placentation.


METHODS
On the morning of conception, pregnant C57BL/6J mice were placed on either of two FA-containing diets: a 3.3 mg health maintenance diet or a high FA diet of 10.5 mg/kg. Mice were injected a binge level of EtOH, HCy, or saline on embryonic day (E) 6.75, targeting gastrulation. On E15.5, cardiac and umbilical blood flow were examined by ultrasound. Embryonic cardiac tissues were processed for gene expression of lipid and FA metabolism; the placenta and heart tissues for neutral lipid droplets, or for medium chain acyl-dehydrogenase (MCAD) protein.


RESULTS
EtOH exposure altered lipid-related gene expression on E7.5 in comparison to control or FA-supplemented groups and remained altered on E15.5 similarly to changes with HCy, signifying FA deficiency. In comparison to control tissues, the lipid-related acyl CoA dehydrogenase medium length chain gene and its protein MCAD were altered with EtOH exposure, as were neutral lipid droplet localization in the heart and placenta.


CONCLUSIONS
EtOH altered gene expression associated with lipid and folate metabolism, as well as neutral lipids, in the E15.5 abnormally functioning heart and placenta. In comparison to controls, the high FA diet protected the embryo and placenta from these effects allowing normal development. Birth Defects Research (Part A) 106:749-760, 2016. © 2016 The Authors Birth Defects Research Part A: Clinical and Molecular Teratology Published by Wiley Periodicals, Inc.
Department of Pediatrics, USF Morsani College of Medicine, Tampa and St. Petersburg, Florida. klinask@health.usf.edu.
Kersti K
KK
Linask
Department of Pediatrics, USF Morsani College of Medicine, Tampa and St. Petersburg, Florida.
Mingda
M
Han
D000818:Animals; D000431:Ethanol / Q000633:toxicity*; D005260:Female; D005492:Folic Acid / Q000494:pharmacology*; D054262:Gastrulation / Q000187:drug effects*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D006321:Heart / Q000196:embryology*; D006330:Heart Defects, Congenital / Q000139:chemically induced / Q000196:embryology / Q000517:prevention & control; D050356:Lipid Metabolism / Q000187:drug effects*; D051379:Mice; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy
D000431:Ethanol; D005492:Folic Acid
MCAD; alcohol; folic acid; heart development; homocysteine; lipid metabolism; mouse embryo; placenta