Structural characteristics are crucial to the benefits of guar gum in experimental osteoarthritis.
Protein-free guar gum (DGG) was oxidized (DGGOX) or sulfated (DGGSU) by insertion of new groups in C-6 (manose) and C-6 (galactose), for DGGOX and DGGSU, respectively. Rats were subjected to anterior cruciate ligament transection (ACLT) of the knee, joint pain recorded using the articular incapacitation test, and the analgesic effect of intraarticular 100μg DGG, DGGOX or DGGSU solutions at days 4-7 was evaluated. Other groups received DGG or saline weekly, from days 7 to 70 and joint damage assessed using histology and biochemistry as the chondroitin sulfate (CS) content of cartilage. The molar mass of CS samples was obtained by comparing their relative electrophoretic mobility to standard CS. DGG but not DGGOX or DGGSU significantly inhibited joint pain. DGG significantly reversed the increase in CS, its reduced electrophoretic mobility, and histological changes following ACLT, as compared to vehicle. Structural integrity accounts for DGG benefits in experimental osteoarthritis.
Superior Institute of Biomedical Sciences, State University of Ceará, Fortaleza 60714-903, Brazil.
Rondinelle R
RR
Castro
Department of Internal Medicine, Federal University of Ceará, Fortaleza 60430-170, Brazil.
Christine Maria M
CM
Silva
Department of Internal Medicine, Federal University of Ceará, Fortaleza 60430-170, Brazil.
Rodolfo M
RM
Nunes
Department of Organic and Inorganic Chemistry, Federal University of Ceará, Fortaleza 60451-970, Brazil.
Pablyana L R
PL
Cunha
Department of Organic and Inorganic Chemistry, Federal University of Ceará, Fortaleza 60451-970, Brazil.
Regina Celia M
RC
de Paula
Department of Organic and Inorganic Chemistry, Federal University of Ceará, Fortaleza 60451-970, Brazil.
Judith P A
JP
Feitosa
Department of Morphology, Faculty of Medicine, Federal University of Ceará, Fortaleza 60430-170, Brazil.
Virgínia C C
VC
Girão
Department of Pathology, Faculty of Medicine, Federal University of Ceará, Fortaleza 60441-750, Brazil.
Margarida M L
MM
Pompeu
Department of Surgery, Federal University of Ceará, Fortaleza 60430-170, Brazil.
José Alberto D
JA
Leite
Department of Internal Medicine, Federal University of Ceará, Fortaleza 60430-170, Brazil. Electronic address: arocha@ufc.br.
Francisco A C
FA
Rocha
D000818:Animals; D018771:Arthralgia / Q000150:complications / Q000188:drug therapy; D002358:Cartilage, Articular / Q000187:drug effects / Q000473:pathology; D004195:Disease Models, Animal; D005685:Galactans / Q000737:chemistry* / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D008351:Mannans / Q000737:chemistry* / Q000494:pharmacology* / Q000627:therapeutic use; D010003:Osteoarthritis / Q000150:complications / Q000188:drug therapy* / Q000473:pathology; D010084:Oxidation-Reduction; D053149:Plant Gums / Q000737:chemistry* / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D013329:Structure-Activity Relationship; D013431:Sulfates / Q000737:chemistry; D014783:Viscosity
D005685:Galactans; D008351:Mannans; D053149:Plant Gums; D013431:Sulfates; C007894:guar gum
Chonsurid (PUBCHEM CID: 24766); Galactomannan (PUBCHEM CID: 439336); Guar gum; Osteoarthritis; Oxidation; Pain; Sulfation