Determination of Aromatic Amines Released from Azo Dyes in Paper Packaging by Liquid Chromatography-Tandem Mass Spectrometry.
An LC-tandem MS (LC-MS/MS) method for the determination of 21 kinds of carcinogenic aromatic amines released from azo dyes in food wrappers was used in this research. Sodium dithionite was added to a citric acid buffer medium to reduce and decompose possible azo dyes. The extract was analyzed after liquid-liquid extraction (LLE) and dispersive SPE (d-SPE). The conditions for chromatographic separation, mass spectrum, LLE, and d-SPE were optimized. Under optimal conditions, the LOD was in the range of 0.13-0.35 mg/kg and LOQ in the range of 0.38-1.05 mg/kg, with the addition of standard recoveries of most aromatic amines being ≥80% and RSDs ≤10%. The recoveries for 2,4-diaminotoluene and 2,4-diaminoanisole were significantly lower, being ≤40%. The method was successfully used to analyze 30 practical samples, and the results showed that it is user-friendly, with high sensitivity, rapid control, and low matrix interference.
China National Tobacco Quality Supervision and Test Center, Zhengzhou 450000, People's Republic of China.
Fei
F
Yang
China National Tobacco Quality Supervision and Test Center, Zhengzhou 450000, People's Republic of China.
Zhaoyang
Z
Bian
China National Tobacco Quality Supervision and Test Center, Zhengzhou 450000, People's Republic of China.
Zhonghao
Z
Li
China National Tobacco Quality Supervision and Test Center, Zhengzhou 450000, People's Republic of China.
Ziyan
Z
Fan
China National Tobacco Quality Supervision and Test Center, Zhengzhou 450000, People's Republic of China.
Ying
Y
Wang
China National Tobacco Quality Supervision and Test Center, Zhengzhou 450000, People's Republic of China.
ShanShan
S
Liu
China National Tobacco Quality Supervision and Test Center, Zhengzhou 450000, People's Republic of China.
Huimin
H
Deng
China National Tobacco Quality Supervision and Test Center, Zhengzhou 450000, People's Republic of China.
Gangling
G
Tang
D000588:Amines / Q000032:analysis*; D001391:Azo Compounds / Q000737:chemistry*; D002853:Chromatography, Liquid; D004396:Coloring Agents / Q000737:chemistry*; D018857:Food Packaging; D010209:Paper; D053719:Tandem Mass Spectrometry
D000588:Amines; D001391:Azo Compounds; D004396:Coloring Agents