Effects of Celangulin IV and V From Celastrus angulatus Maxim on Na+/K+-ATPase Activities of the Oriental Armyworm (Lepidoptera: Noctuidae).
Na(+)/K(+)-ATPase (sodium pump) is an important target for the development of botanical pesticide as it is responsible for transforming chemical energy in ATP to osmotic work and maintaining electrochemical Na(+ )and K(+ )gradients across the cell membrane of most animal cells. Celangulin IV (C-IV) and V (C-V), which are isolated from the root bark of Celastrus angulatus, are the major active ingredients of this insecticidal plant. The activities of C-IV and C-V on Na(+)/K(+)-ATPase were investigated by ultramicro measuring method to evaluate the effects of C-IV and C-V on Na(+)/K(+)-ATPase activities of the brain from the fifth Mythimna separata larvae and to discuss the insecticidal mechanism of C-IV and C-V. Results indicate that inhibitory activities of Na(+)/K(+)-ATPase by C-IV and C-V possess an obvious concentration-dependent in vitro. Compared with C-IV, the inhibition of C-V on Na(+)/K(+)-ATPase was not striking. In vivo, at a concentration of 25 mg/liter, the inhibition ratio of C-IV on Na(+)/K(+)-ATPase activity from the brain in narcosis and recovery period was more remarkable than that of C-V. Furthermore, the insects were fed with different mixture ratios of C-IV and C-V. The inhibition extent of Na(+)/K(+)-ATPase activity was corresponded with the dose of C-IV. However, C-V had no notable effects. This finding may mean that the mechanism of action of C-IV and C-V on Na(+)/K(+)-ATPase were different. Na(+)/K -ATPase may be an action target of C-IV and C-V.
College of Plant Protection, Institute of Pesticide Science, Northwest A&F University, Yangling, Shaanxi 712100, China (chendannwsuaf@126.com; fengmx2010@126.com; jyf_1991@126.com; wuwenjun@nwsuaf.edu.cn; huzhaonong@nwsuaf.edu.cn) Key Laboratory of Botanical Pesticide R & D in Shaanxi Province, Yangling, Shaanxi 712100, China.
Dan
D
Cheng
College of Plant Protection, Institute of Pesticide Science, Northwest A&F University, Yangling, Shaanxi 712100, China (chendannwsuaf@126.com; fengmx2010@126.com; jyf_1991@126.com; wuwenjun@nwsuaf.edu.cn; huzhaonong@nwsuaf.edu.cn) Key Laboratory of Botanical Pesticide R & D in Shaanxi Province, Yangling, Shaanxi 712100, China.
Mingxing
M
Feng
College of Plant Protection, Institute of Pesticide Science, Northwest A&F University, Yangling, Shaanxi 712100, China (chendannwsuaf@126.com; fengmx2010@126.com; jyf_1991@126.com; wuwenjun@nwsuaf.edu.cn; huzhaonong@nwsuaf.edu.cn) Key Laboratory of Botanical Pesticide R & D in Shaanxi Province, Yangling, Shaanxi 712100, China.
Yufei
Y
Ji
College of Plant Protection, Institute of Pesticide Science, Northwest A&F University, Yangling, Shaanxi 712100, China (chendannwsuaf@126.com; fengmx2010@126.com; jyf_1991@126.com; wuwenjun@nwsuaf.edu.cn; huzhaonong@nwsuaf.edu.cn) Key Laboratory of Botanical Pesticide R & D in Shaanxi Province, Yangling, Shaanxi 712100, China.
Wenjun
W
Wu
College of Plant Protection, Institute of Pesticide Science, Northwest A&F University, Yangling, Shaanxi 712100, China (chendannwsuaf@126.com; fengmx2010@126.com; jyf_1991@126.com; wuwenjun@nwsuaf.edu.cn; huzhaonong@nwsuaf.edu.cn) Key Laboratory of Botanical Pesticide R & D in Shaanxi Province, Yangling, Shaanxi 712100, China huzhaonong@nwsuaf.edu.cn.
Zhaonong
Z
Hu
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D031247:Celastrus / Q000737:chemistry*; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006241:Haptens / Q000494:pharmacology*; D019476:Insect Proteins / Q000037:antagonists & inhibitors*; D007306:Insecticides / Q000494:pharmacology; D007814:Larva / Q000187:drug effects / Q000201:enzymology; D009036:Moths / Q000187:drug effects* / Q000201:enzymology; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*
D004791:Enzyme Inhibitors; D006241:Haptens; D019476:Insect Proteins; D007306:Insecticides; C514792:celangulin V; D000254:Sodium-Potassium-Exchanging ATPase
Celangulin; Mythimna separata; Na+/K+-ATPase; target