Evaluation and study of antimicrobial activity of nanoliposomal meropenem against Pseudomonas aeruginosa isolates.
BACKGROUND
The aim of this study was to evaluate the antimicrobial efficacy of liposomal meropenem formulation against clinical and laboratory isolates of Pseudomonas aeruginosa. P. aeruginosa isolates were used to determine the MIC and MBC of free and liposomal meropenem. As well as the effect of free and liposomes meropenem compare the minimum biofilm eradication concentration and inhibition of P. aeruginosa motility was assessed.


RESULTS
In this study, the MICs and MBC of liposomal meropenem was more effective against all clinical and laboratory strains were significantly lower than those of free meropenem (6.25 μg/ml vs 100 μg/ml). Furthermore, liposomal meropenem (≥1.5 μg/ml) completely eradicated biofilm in all P. aeruginosa isolates. While the free form of drug could eradicate biofilm formation in most of isolates in ≥6.25 μg/ml concentration. In addition, lower concentrations of liposomal meropenem could inhibit the bacterial motility.


CONCLUSIONS
The results clearly indicate that the liposomal formulation of drug is more effective than meropenem alone against antibiotic resistant P. aeruginosa isolates.
a Department of Medical Biotechnology, Faculty of Advanced Medical Science , Tabriz University of Medical Science , Tabriz , Iran.
Momeni-Javid
MJ
Zahra
b Drug Applied Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.
Hamishekar
H
Hamed
a Department of Medical Biotechnology, Faculty of Advanced Medical Science , Tabriz University of Medical Science , Tabriz , Iran.|b Drug Applied Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.
Rahmati-Yamchi
RY
Mohammad
a Department of Medical Biotechnology, Faculty of Advanced Medical Science , Tabriz University of Medical Science , Tabriz , Iran.
Zarghami
Z
Nosratollah
b Drug Applied Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.
Abolfazl
A
Akbarzadeh
b Drug Applied Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.|c Liver and Gastrointestinal Disease Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.
Milani
M
Morteza
D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development; D004339:Drug Compounding; D024881:Drug Resistance, Bacterial / Q000187:drug effects; D008081:Liposomes / Q000737:chemistry*; D000077731:Meropenem; D008826:Microbial Sensitivity Tests; D009068:Movement; D049329:Nanostructures / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000502:physiology; D013845:Thienamycins / Q000737:chemistry* / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D008081:Liposomes; D013845:Thienamycins; D000077731:Meropenem
Antibiotic resistance; P. aeruginosa; liposomal meropenem