Synthesis and Photophysical Properties of Fluorescence Molecular Probe for Turn-ON-Type Detection of Cytosine Bulge DNA.
A fluorescent molecule DANP that binds to cytosine bulge DNA and emits characteristic fluorescence was conjugated to pyrene to give a new fluorescence probe PyDANP. Temperature- and solvent-dependent absorption changes showed that DANP and pyrene chromophores stacked at room temperature in an aqueous buffer solution and quenched fluorescence. Upon binding of DANP moiety in PyDANP to cytosine bulge DNA, the fluorescence from DANP bound to C-bulge increased by ∼12-fold, showing that PyDANP is a turn-ON probe for the detection of C-bulge DNA.
Department of Regulatory Bioorganic Chemistry, The Institute of Scientific and Industrial Research, Osaka University , Mihogaoka, 8-1, Ibaraki, Osaka 567-0047, Japan.
Rajiv
R
Kumar Verma
National Defense Medical College , Namiki, 3-2, Tokorozawa, Saitama 359-8513, Japan.
Fumie
F
Takei
Department of Regulatory Bioorganic Chemistry, The Institute of Scientific and Industrial Research, Osaka University , Mihogaoka, 8-1, Ibaraki, Osaka 567-0047, Japan.
Kazuhiko
K
Nakatani
D001483:Base Sequence; D003596:Cytosine / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D055029:Inverted Repeat Sequences; D015335:Molecular Probes / Q000138:chemical synthesis*; D009287:Naphthyridines / Q000138:chemical synthesis*; D009690:Nucleic Acid Conformation; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling
D005456:Fluorescent Dyes; D015335:Molecular Probes; D009287:Naphthyridines; D003596:Cytosine; D004247:DNA