Microfluidic Devices for the Measurement of Cellular Secretion.
The release of chemical information from cells and tissues holds the key to understanding cellular behavior and dysfunction. The development of methodologies that can measure cellular secretion in a time-dependent fashion is therefore essential. Often these measurements are made difficult by the high-salt conditions of the cellular environment, the presence of numerous other secreted factors, and the small mass samples that are produced when frequent sampling is used to resolve secretory dynamics. In this review, the methods that we have developed for measuring hormone release from islets of Langerhans are dissected to illustrate the practical difficulties of studying cellular secretions. Other methods from the literature are presented that provide alternative approaches to particularly challenging areas of monitoring cellular secretion. The examples presented in this review serve as case studies and should be adaptable to other cell types and systems for unique applications.
Department of Chemistry and Biochemistry, Florida State University, Tallahassee, Florida 32306; email: roper@chem.fsu.edu.
Adrian M
AM
Schrell
Department of Chemistry and Biochemistry, Florida State University, Tallahassee, Florida 32306; email: roper@chem.fsu.edu.
Nikita
N
Mukhitov
Department of Chemistry and Biochemistry, Florida State University, Tallahassee, Florida 32306; email: roper@chem.fsu.edu.
Lian
L
Yi
Department of Chemistry and Biochemistry, Florida State University, Tallahassee, Florida 32306; email: roper@chem.fsu.edu.
Xue
X
Wang
Department of Chemistry and Biochemistry, Florida State University, Tallahassee, Florida 32306; email: roper@chem.fsu.edu.
Michael G
MG
Roper
D000818:Animals; D002477:Cells / Q000378:metabolism*; D006728:Hormones / Q000378:metabolism; D006801:Humans; D007515:Islets of Langerhans / Q000378:metabolism; D046210:Microfluidic Analytical Techniques
D006728:Hormones
integrated; lab-on-a-chip; micro total analysis system; perfusion; release