An Al-inducible expansin gene, OsEXPA10 is involved in root cell elongation of rice.
Expansins are cell wall loosening proteins, which are encoded by multigene families. However, the physiological role of most expansin genes is still poorly understood. Here, we functionally characterized an Al-inducible expansin gene, OsEXPA10, which is regulated by a C2H2-type zinc-finger transcription factor, ART1 in rice. A detailed expression analysis showed that OsEXPA10 was expressed in both the roots and shoots at a similar level, but only the expression in the roots was rapidly upregulated in response to Al. Furthermore, spatial expression analysis showed that the Al-induced expression was only found in the root tips (0-3 mm), but not in the mature root zones. The expression was neither induced by other metals including Cd and La nor by low pH. Immunostaining showed that OsEXPA10 was localized at all cells of the root tips. Knockout of OsEXPA10 resulted in a significant decrease in the cell elongation of the roots in the absence of Al. In the presence of Al, knockout of OsEXPA10 did not alter the Al sensitivity evaluated by relative root elongation, but the root cell wall of knockout lines accumulated less Al compared to those of the wild-type rice. Collectively, our results indicate that OsEXPA10 expressed in the root tips is required for the root cell elongation, but that the contribution of this gene to high Al tolerance in rice is small.
State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China.|University of Chinese Academy of Sciences, Beijing, 100049, China.|Institute of Plant Science and Resources, Okayama University, Chuo 2-20-1, Kurashiki, 710-0046, Japan.
Jing
J
Che
Institute of Plant Science and Resources, Okayama University, Chuo 2-20-1, Kurashiki, 710-0046, Japan.
Naoki
N
Yamaji
State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China. rfshen@issas.ac.cn.
Ren Fang
RF
Shen
State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China. maj@rib.okayama-u.ac.jp.|Institute of Plant Science and Resources, Okayama University, Chuo 2-20-1, Kurashiki, 710-0046, Japan. maj@rib.okayama-u.ac.jp.
Jian Feng
JF
Ma
D000535:Aluminum / Q000633:toxicity; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D018519:Meristem / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D012275:Oryza / Q000187:drug effects / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000502:physiology
D010940:Plant Proteins; D000535:Aluminum
aluminum toxicity; cell elongation; expansin; expression pattern; rice; root tip, Oryza sativa