The role of photobiomodulation on gene expression of cell adhesion molecules in diabetic wounded fibroblasts in vitro.
Cell adhesion molecules (CAMs) are cell surface glycoproteins that facilitate cell-cell contacts and adhesion with the extracellular matrix (ECM). Cellular adhesion is affected by various disease conditions, such as diabetes mellitus (DM) and inflammation. Photobiomodulation (PBM) stimulates biological processes and expression of these cellular molecules. The aim of this experimental work was to demonstrate the role of PBM at 830nm on CAMs in diabetic wounded fibroblast cells. Isolated human skin fibroblast cells were used. Normal (N-) and diabetic wounded (DW-) cells were irradiated with a continuous wave diode laser at 830nm with an energy density of 5J/cm(2). Real time reverse transcriptase polymerase chain reaction (RT-PCR) was used to determine the relative gene expression of 39 CAMs 48h post-irradiation. Normalized expression levels from irradiated cells were calculated relative to non-irradiated control cells according to the 2^(-ΔΔCt) method. Thirty-one genes were significantly regulated in N-cells (28 were genes up-regulated and three genes down-regulated), and 22 genes in DW-cells (five genes were up-regulated and 17 genes down-regulated). PBM induced a stimulatory effect on various CAMs namely cadherins, integrins, selectins and immunoglobulins, and hence may be used as a complementary therapy in advancing treatment of non-healing diabetic ulcers. The regulation of CAMs as well as evaluating the role of PBM on the molecular effects of these genes may expand knowledge and prompt further research into the cellular mechanisms in diabetic wound healing that may lead to valuable clinical outcomes.
Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, P.0. Box 17011, Doornfontein 2028, South Africa. Electronic address: matabs63@yahoo.com.
Sandra M
SM
Ayuk
Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, P.0. Box 17011, Doornfontein 2028, South Africa. Electronic address: habrahamse@uj.ac.za.
Heidi
H
Abrahamse
Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, P.0. Box 17011, Doornfontein 2028, South Africa. Electronic address: nhoureld@uj.ac.za.
Nicolette N
NN
Houreld
D000328:Adult; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000378:metabolism; D002478:Cells, Cultured; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism / Q000473:pathology; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects; D015870:Gene Expression / Q000528:radiation effects*; D006801:Humans; D054023:Lasers, Semiconductor; D060888:Real-Time Polymerase Chain Reaction
D015815:Cell Adhesion Molecules
Cellular adhesion molecules; Diabetes; Extracellular matrix; Laser; Wound healing