Quantification of polyhydroxyalkanoates in mixed and pure cultures biomass by Fourier transform infrared spectroscopy: comparison of different approaches.
Fourier transform infrared (FTIR) spectroscopy was used in this study for the rapid quantification of polyhydroxyalkanoates (PHA) in mixed and pure culture bacterial biomass. Three different statistical analysis methods (regression, partial least squares (PLS) and nonlinear) were applied to the FTIR data and the results were plotted against the PHA values measured with the reference gas chromatography technique. All methods predicted PHA content in mixed culture biomass with comparable efficiency, indicated by similar residuals values. The PHA in these cultures ranged from low to medium concentration (0-44 wt% of dried biomass content). However, for the analysis of the combined mixed and pure culture biomass with PHA concentration ranging from low to high (0-93% of dried biomass content), the PLS method was most efficient. This paper reports, for the first time, the use of a single calibration model constructed with a combination of mixed and pure cultures covering a wide PHA range, for predicting PHA content in biomass.


CONCLUSIONS
Currently no one universal method exists for processing FTIR data for polyhydroxyalkanoates (PHA) quantification. This study compares three different methods of analysing FTIR data for quantification of PHAs in biomass. A new data-processing approach was proposed and the results were compared against existing literature methods. Most publications report PHA quantification of medium range in pure culture. However, in our study we encompassed both mixed and pure culture biomass containing a broader range of PHA in the calibration curve. The resulting prediction model is useful for rapid quantification of a wider range of PHA content in biomass.
Scion, Rotorua, New Zealand.
I
I
Isak
Scion, Rotorua, New Zealand.
M
M
Patel
Scion, Rotorua, New Zealand.
M
M
Riddell
Scion, Rotorua, New Zealand.
M
M
West
Scion, Rotorua, New Zealand.
T
T
Bowers
Scion, Rotorua, New Zealand.
S
S
Wijeyekoon
Scion, Rotorua, New Zealand.
J
J
Lloyd
D000217:Acyltransferases / Q000235:genetics; D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D054813:Polyhydroxyalkanoates / Q000032:analysis* / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
D054813:Polyhydroxyalkanoates; D000217:Acyltransferases; C077225:poly(3-hydroxyalkanoic acid) synthase
Fourier transform infrared spectroscopy; biopolymers; biotransformation; fermentation; polyhydroxyalkanoates