Anti-Human Endoglin (hCD105) Immunotoxin-Containing Recombinant Single Chain Ribosome-Inactivating Protein Musarmin 1.
Endoglin (CD105) is an accessory component of the TGF-β receptor complex, which is expressed in a number of tissues and over-expressed in the endothelial cells of tumor neovasculature. Targeting endoglin with immunotoxins containing type 2 ribosome-inactivating proteins has proved an effective tool to reduce blood supply to B16 mice tumor xenografts. We prepared anti-endoglin immunotoxin (IT)-containing recombinant musarmin 1 (single chain ribosome-inactivating proteins) linked to the mouse anti-human CD105 44G4 mouse monoclonal antibody via N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP). The immunotoxin specifically killed L929 fibroblast mouse cells transfected with the short form of human endoglin with IC50 values in the range of 5 × 10(-10) to 10(-9) M.
Department of Biochemistry and Molecular Biology, University of Valladolid, 47005 Valladolid, Spain. barmagma@jcyl.es.
Begoña
B
Barriuso
Department of Biochemistry and Molecular Biology, University of Valladolid, 47005 Valladolid, Spain. mantolin@umh.es.
Pilar
P
Antolín
Department of Biochemistry and Molecular Biology, University of Valladolid, 47005 Valladolid, Spain. arias@bioforge.uva.es.|Bioforge, University of Valladolid, Spain and Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 47002 Valladolid, Spain. arias@bioforge.uva.es.
F Javier
FJ
Arias
Bioforge, University of Valladolid, Spain and Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 47002 Valladolid, Spain. agirotti@bioforge.uva.es.
Alessandra
A
Girotti
Department of Nutrition and Bromatology, University of Valladolid, 47005 Valladolid, Spain. pilarj@bio.uva.es.
Pilar
P
Jiménez
Department of Pharmacy &amp; Pharmaceutical Technology and University Institute of Industrial Pharmacy (IUFI), Complutense University of Madrid, 28040 Madrid, Spain. mcordoba@farm.ucm.es.
Manuel
M
Cordoba-Diaz
Department of Pharmacy &amp; Pharmaceutical Technology and University Institute of Industrial Pharmacy (IUFI), Complutense University of Madrid, 28040 Madrid, Spain. damianco@farm.ucm.es.
Damián
D
Cordoba-Diaz
Department of Nutrition and Bromatology, University of Valladolid, 47005 Valladolid, Spain. girbes@bio.uva.es.
Tomás
T
Girbés
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D000071063:Endoglin / Q000276:immunology*; D006801:Humans; D000922:Immunotoxins / Q000494:pharmacology*; D051379:Mice; D009699:N-Glycosyl Hydrolases / Q000494:pharmacology*
D000071063:Endoglin; D000922:Immunotoxins; D009699:N-Glycosyl Hydrolases; C494106:musarmin 1, Muscari armeniacum
44G4; CD105; anti-endoglin monoclonal antibody; anti-tumor therapy; cancer therapy; endoglin; recombinant musarmin