Tobacco Etch Virus protease: A shortcut across biotechnologies.
About thirty years ago, studies on the RNA genome of Tobacco Etch Virus revealed the presence of an efficient and specific protease, called Tobacco Etch Virus protease (TEVp), that was part of the Nuclear Inclusion a (NIa) enzyme. TEVp is an efficient and specific protease of 27kDa that has become a valuable biotechnological tool. Nowadays TEVp is a unique endopeptidase largely exploited in biotechnology from industrial applications to in vitro and in vivo cellular studies. A number of TEVp mutants with different rate of cleavage, stability and specificity have been reported. Similarly, a panel of different target cleavage sites, derived from the canonical ENLYFQ-G/S site, has been established. In this review we describe these aspects of TEVp and some of its multiple applications. A particular focus is on the use and molecular biology of TEVp in living cells and organisms.
International Centre for Genetic Engineering and Biotechnology, ICGEB, Trieste, Italy.
Francesca
F
Cesaratto
International Centre for Genetic Engineering and Biotechnology, ICGEB, Trieste, Italy. Electronic address: burrone@icgeb.org.
Oscar R
OR
Burrone
CIBIO, University of Trento, Trento, Italy. Electronic address: gianluca.petris@unitn.it.
Gianluca
G
Petris
D001709:Biotechnology / Q000379:methods*; D010450:Endopeptidases; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015202:Protein Engineering / Q000379:methods*
D010450:Endopeptidases; C099488:TEV protease
In vivo applications; Protein engineering; Self-cleavage; Split-TEV; Subcellular targeting; TEV protease