Down-Regulation of miR-19a as a Biomarker for Early Detection of Silicosis.
The accumulated data indicate that there is significant genetic heterogeneity underlying the etiology of silicosis. Recent reports have revealed that microRNAs (miRNAs) play an important role in regulating pulmonary fibrosis. This study, therefore, aimed to identify some miRNAs as biomarkers for silicosis, and to explore the early diagnostic value of biomarkers for silicosis. Total RNAs were collected from the peripheral blood leukocytes of 23 silicosis patients and 23 healthy controls, the different miRNAs were screened using microarrays. The potential biomarker miRNAs were identified by quantitative real-time polymerase chain reaction (qPCR) and receiver operating characteristic (ROC) curves. Eighteen differential miRNAs in leukocytes were up-regulated and twenty differential miRNAs were down-regulated in the silicosis group, compared with the control group. The expression levels of miR-181a and miR-19a were 0.8854 ± 0.1037 and 0.2929 ± 0.0342 by the relative quantitation method 2(-△△CT) of qPCR, respectively. The sensitivity and specificity for miR-181a at a cut-off value of 1.8917 were 70% and 75%, respectively, whereas, those for miR-19a at a cut-off value of 3.6828 were 95% and 95%, respectively. Thus, miR-19a in peripheral blood leukocyte could be used as an effective biomarker for silicosis. Anat Rec, 299:1300-1307, 2016. © 2016 Wiley Periodicals, Inc.
Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazard, No. 1 Huizhihuan Road, Dongli District, Tianjin, 300309, China.|Institute of Medical Equipment, Academy of Military Medical Sciences of Chinese People's Libration Army, 106 Wan Dong Road, Hedong District, Tianjin, 300163, China.|Department of Hygiene, Logistics College of Chinese People's Armed Police Forces, 1 Huizhihuan Road, Dongli District, Tianjin, 300309, China.
Zhen
Z
Yang
Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazard, No. 1 Huizhihuan Road, Dongli District, Tianjin, 300309, China.
Qian
Q
Li
School of Public Health, Xinxiang Medical University, 601 Jinsui Road, Hongqi District, Henan, 453003, China.
Sanqiao
S
Yao
Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazard, No. 1 Huizhihuan Road, Dongli District, Tianjin, 300309, China.
Ge
G
Zhang
Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazard, No. 1 Huizhihuan Road, Dongli District, Tianjin, 300309, China.
Rong
R
Xue
Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazard, No. 1 Huizhihuan Road, Dongli District, Tianjin, 300309, China.|Department of Hygiene, Logistics College of Chinese People's Armed Police Forces, 1 Huizhihuan Road, Dongli District, Tianjin, 300309, China.
Guoliang
G
Li
Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazard, No. 1 Huizhihuan Road, Dongli District, Tianjin, 300309, China.|Department of Hygiene, Logistics College of Chinese People's Armed Police Forces, 1 Huizhihuan Road, Dongli District, Tianjin, 300309, China.
Yizheng
Y
Wang
Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazard, No. 1 Huizhihuan Road, Dongli District, Tianjin, 300309, China.
Shixin
S
Wang
Institute of Medical Equipment, Academy of Military Medical Sciences of Chinese People's Libration Army, 106 Wan Dong Road, Hedong District, Tianjin, 300163, China.
Ruichang
R
Wu
Tianjin Key Laboratory for Prevention and Control of Occupational and Environmental Hazard, No. 1 Huizhihuan Road, Dongli District, Tianjin, 300309, China.|Department of Hygiene, Logistics College of Chinese People's Armed Police Forces, 1 Huizhihuan Road, Dongli District, Tianjin, 300309, China.
Hongsheng
H
Gao
D015415:Biomarkers / Q000378:metabolism; D015536:Down-Regulation; D042241:Early Diagnosis; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D012680:Sensitivity and Specificity; D012829:Silicosis / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism
D015415:Biomarkers; C549214:MIRN19A microRNA, human; D035683:MicroRNAs
biomarker; miR-19a; silicosis