Association of single nucleotide polymorphisms in pro-inflammatory cytokine and toll-like receptor genes with pediatric hematogenous osteomyelitis.
Hematogenous osteomyelitis (HO) is a bone infection wherein bacteria penetrate to the bone through the blood stream. Several single nucleotide polymorphisms (SNPs) have been associated with susceptibility to infectious diseases. In this study, we investigated the contribution of SNPs in interleukin (IL)-1B1 (rs16944), IL1A (rs1800587), IL1B (rs1143634), toll-like receptor (TLR)-2 (rs3804099), TLR4 (rs4986790), TLR4 (rs4986791), IL1R (rs2234650), tumor necrosis factor (TNF)-α (rs1800629), TNF (rs361525), and IL1RN (rs315952) towards the development of HO in Saudi patients and compared to healthy controls. Fifty-two patients diagnosed with HO and 103 healthy individuals were genotyped. The frequencies of genotypes GG (rs16944) and AA (rs16944) were lower and higher in patients [odds ratio (OR) = 0.34, Pc = 0.05] and controls (OR = 1.33, Pc = 0.05), respectively, suggesting that SNPs at this locus could alter HO susceptibility. In addition, the patients and controls exhibited lower and higher frequencies of the alleles G (rs16944) (OR = 0.43, Pc = 0.007) and A (rs16944) (OR = 2.32, Pc = 0.007), respectively. The expression of alleles C (rs3804099) and T (rs3804099) were higher in patients (OR = 2.05, Pc = 0.04) and controls (OR = 0.49, Pc = 0.04), respectively. In conclusion, SNPs at rs16944 and rs3804099 were found to be associated with HO in the Saudi population.
King Fahad Medical City, Riyadh, Saudi Arabia.
A E
AE
Osman
King Fahad Medical City, Riyadh, Saudi Arabia.
M
M
Mubasher
King Fahad Medical City, Riyadh, Saudi Arabia.
N E
NE
ElSheikh
King Fahad Medical City, Riyadh, Saudi Arabia.
H
H
AlHarthi
King Fahad Medical City, Riyadh, Saudi Arabia.
M S
MS
AlZahrani
King Fahad Medical City, Riyadh, Saudi Arabia.
N
N
Ahmed
Mafraq hospital and Sheikh Khalifa Medical city, Abu Dhabi, United Arab Emirates.
G
G
ElGhazali
Musculoskeletal Research Unit, First Floor Learning and Research Building, Southmead Hospital, University of Bristol, Bristol, United Kingdom.
B A
BA
Bradley
King Fahad Medical City, Riyadh, Saudi Arabia.
A-S A
AS
Fadil
D000483:Alleles; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein / Q000235:genetics; D053582:Interleukin-1alpha / Q000235:genetics; D053583:Interleukin-1beta / Q000235:genetics*; D008297:Male; D010019:Osteomyelitis / Q000235:genetics* / Q000473:pathology; D053573:Receptors, Interleukin-1 Type I / Q000235:genetics; D012529:Saudi Arabia; D051195:Toll-Like Receptor 2 / Q000235:genetics*; D051197:Toll-Like Receptor 4 / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
C508584:IL1A protein, human; C508590:IL1R1 protein, human; C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D053582:Interleukin-1alpha; D053583:Interleukin-1beta; D053573:Receptors, Interleukin-1 Type I; C495345:TLR4 protein, human; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha