Broad-band excitation in indirectly detected (14)N overtone spectroscopy with composite pulses.
We show here that composite pulses allow broad-band excitation of nitrogen-14 overtone frequencies in proton detected D-HMQC experiments (referred to (1)H-{NOTDQ14} D-HMQC). Experimental verifications have been performed on glycine, L-histidine and N-acetyl-valine (NAV) samples. Composite pulses enable symmetric excitations of (14)N sites with large shift differences. Therefore, this approach is promising for recording high resolution (1)H-{NOTDQ14} D-HMQC spectra of most amino-acids, pharmaceutical samples and peptides.
Shanghai Key Laboratory of Magnetic Resonance, School of Physics and Materials Science, East China Normal University, Shanghai 200062, China. Electronic address: shen.ming@outlook.com.
Ming
M
Shen
Shanghai Key Laboratory of Magnetic Resonance, School of Physics and Materials Science, East China Normal University, Shanghai 200062, China.
Qun
Q
Chen
Shanghai Key Laboratory of Magnetic Resonance, School of Physics and Materials Science, East China Normal University, Shanghai 200062, China.
Jean-Paul
JP
Amoureux
Shanghai Key Laboratory of Magnetic Resonance, School of Physics and Materials Science, East China Normal University, Shanghai 200062, China. Electronic address: bwhu@phy.ecnu.edu.cn.
Bingwen
B
Hu
D005998:Glycine; D006639:Histidine; D009682:Magnetic Resonance Spectroscopy; D009584:Nitrogen; D011522:Protons
D011522:Protons; D006639:Histidine; D009584:Nitrogen; D005998:Glycine
(14)N overtone spectroscopy; Proton-detected D-HMQC; Solid-state NMR