[Production effect comparison of SEPP and GPx between HepG2 and Hela cells with different selenocompounds].
OBJECTIVE
To compare the effect of several selenocompounds on the productions of SEPP and GPx in HepG2 and Hela cells.


METHODS
The cultured HepG2 and Hela cells were divided into the control, Na2SeO3, SeMet and MeSeCys groups. After adding the selected selenocompounds (with the respective concentration 0.01 and 0.1 μmol/L), the experimental groups were then incubated for 48 h and 72 h. Finally, the cell culture supernatants and homogenates were collected for the SEPP and GPx concentrations detection by a double-antibody sandwich enyme-linked immuno-sorbent-assay (ELISA).


RESULTS
The SEPP and GPx concentrations in Hela cells treated with 0.1 μmol/L SeMet and MeSeCys were significantly higher than that in the control group (P < 0.05). The SEPP and GPx concentrations in HepG2 cell treated with 0.1 μmol/L selenocompounds were significantly higher than that in Hela cells (P < 0.05).


CONCLUSIONS
HepG2 cells are more beneficial to the production of selenoproteins than Hela cells.
Qin
Q
Wang
Lina
L
Gao
Feng
F
Han
Jiaxi
J
Lu
Yiqun
Y
Liu
Licui
L
Sun
Zhenwu
Z
Huang
D005978:Glutathione / Q000378:metabolism*; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D018036:Selenium Compounds / Q000737:chemistry*; D017279:Selenocysteine / Q000031:analogs & derivatives; D012645:Selenomethionine; D051140:Selenoproteins / Q000378:metabolism*
D018036:Selenium Compounds; D051140:Selenoproteins; D017279:Selenocysteine; D012645:Selenomethionine; D005978:Glutathione; C002979:selenomethylselenocysteine