In Vivo Magnetic Resonance and Fluorescence Dual-Modality Imaging of Tumor Angiogenesis in Rats Using GEBP11 Peptide Targeted Magnetic Nanoparticles.
Angiogenesis is an essential process for tumor progression. Tumor vasculature-targeting peptides have shown great potential for use in cancer imaging and therapy. Our previous studies have shown that GEBP11, a novel vasculature-specific binding peptide that exhibits high affinity and specificity to tumor angiogenesis, is a promising candidate for the diagnosis and targeted radiotherapy of gastric cancer. In the present study, we developed a novel magnetic resonance and fluorescence (MR/Fluo) dual-modality imaging probe by covalently coupling 2,3-dimercaptosuccinnic acid-coated paramagnetic nanoparticles (DMSA-MNPs) and Cy5.5 to the GEBP11 peptide. The probe Cy5.5-GEBP11-DMSA-MNPs (CGD-MNPs), with a hydrodynamic diameter of 82.8 ± 6.5 nm, exhibited good imaging properties, high stability and little cytotoxicity. In vivo MR/Fluo imaging revealed that CGD-MNPs were successfully applied to visualize tumor angiogenesis in SGC-7901 xenograft mouse models. Prussian blue and CD31 immunohistochemical staining confirmed that CGD-MNPs co-localized with tumor blood vessels. In conclusion, CGD-MNPs are promising candidates for use as MR and fluorescence imaging probes for visualizing gastric cancer angiogenesis in vivo.
Tao
T
Su
Yabin
Y
Wang
Jiinda
J
Wang
Dong
D
Han
Sai
S
Ma
Jianbo
J
Cao
Xiujuan
X
Li
Ran
R
Zhang
Hongyu
H
Qiao
Jimin
J
Liang
Gang
G
Liu
Bo
B
Yang
Shuhui
S
Liang
Yongzhan
Y
Nie
Kaichun
K
Wu
Jiayi
J
Li
Feng
F
Cao
D000818:Animals; D002232:Carbocyanines / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects; D003952:Diagnostic Imaging; D004705:Endocytosis / Q000187:drug effects; D005453:Fluorescence; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D008819:Mice, Nude; D009369:Neoplasms / Q000098:blood supply* / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000175:diagnosis*; D010455:Peptides / Q000494:pharmacology*; D051381:Rats; D004113:Succimer / Q000378:metabolism; D014018:Tissue Distribution / Q000187:drug effects
C098793:CY5.5 cyanine dye; D002232:Carbocyanines; C000608690:GEBP11 peptide; D058185:Magnetite Nanoparticles; D010455:Peptides; D004113:Succimer