Delaminated Layered Double Hydroxide Nanosheets as an Efficient Vector for DNA Delivery.
The performance of delaminated Mg-Al-lactate and Mg-Al-acetate layered double hydroxides (LDHs) nanosheets (Mg-Al-lactate-NS, Mg-Al-acetate-NS) as efficient vectors for DNA adsorption and delivery to 293T cells was investigated. Mg-Al-lactate and Mg-Al-acetate LDHs were delaminated into single layers in water and were characterized using XRD, SEM, TEM, and Zeta potential measurements. The salmon sperm DNA adsorption capacity of Mg-Al-lactate-NS and Mg-Al-acetate-NS were evaluated by varying the adsorbent dosage and contacting time, which suggested that Mg-Al-lactate-NS had much higher adsorption capacity (649.6 μg mg-1) than that of Mg-Al-acetate-NS (340.0 μg mg(-1)). XRD analysis indicated that after DNA adsorption the Mg-Al-lactate-NS-DNA bio-inorganic nanohybrid still stayed in an exfoliated form. Due to the difficulty in separating the Mg-Al-lactate-NS-DNA from solution, electrophoresis analysis was also applied to detect the free DNA in solution after adsorption. Cytotoxicity studies using 293T cells verified that Mg-Al-lactate-NS was less toxic than Mg-Al-acetate-NS as a smaller dose of this LDH was needed to deliver the same amount of salmon DNA to 293T cells. Cellular uptake and confocal imaging studies demonstrated that Mg-Al-lactate-NS was successful in transfection of ssDNA-FITC into 293T cells. However, the FITC-coupled single strand DNA was failed to be internalized into these cells. The excellent DNA adsorption and delivery capacities indicate that delaminated Mg-Al-lactate LDHs nanosheets are a better DNA vector than bulk phase LDH.
Junya
J
Wang
Wenlong
W
Bao
Ahmad
A
Umar
Qiang
Q
Wang
Dermot
D
O'Hare
Yinglang
Y
Wan
D000327:Adsorption; D000535:Aluminum / Q000737:chemistry; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000008:administration & dosage*; D004587:Electrophoresis, Agar Gel; D004705:Endocytosis / Q000187:drug effects; D005434:Flow Cytometry; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000378:metabolism*; D006801:Humans; D006878:Hydroxides / Q000737:chemistry* / Q000633:toxicity; D007700:Kinetics; D019344:Lactic Acid / Q000737:chemistry; D008274:Magnesium / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D013997:Time Factors; D014961:X-Ray Diffraction
D006878:Hydroxides; D019344:Lactic Acid; D004247:DNA; D000535:Aluminum; D008274:Magnesium