Imaging collective cell migration and hair cell regeneration in the sensory lateral line.
The accessibility of the lateral line system and its amenability to long-term in vivo imaging transformed the developing lateral line into a powerful model system to study fundamental morphogenetic events, such as guided migration, proliferation, cell shape changes, organ formation, organ deposition, cell specification and differentiation. In addition, the lateral line is not only amenable to live imaging during migration stages but also during postembryonic events such as sensory organ tissue homeostasis and regeneration. The robust regenerative capabilities of the mature, mechanosensory lateral line hair cells, which are homologous to inner ear hair cells and the ease with which they can be imaged, have brought zebrafish into the spotlight as a model to develop tools to treat human deafness. In this chapter, we describe protocols for long-term in vivo confocal imaging of the developing and regenerating lateral line.
Stowers Institute for Medical Research, Kansas City, MO, United States; University of Utah, Salt Lake City, UT, United States.
M
M
Venero Galanternik
Stowers Institute for Medical Research, Kansas City, MO, United States.
J
J
Navajas Acedo
Stowers Institute for Medical Research, Kansas City, MO, United States; University of Utah, Salt Lake City, UT, United States.
A
A
Romero-Carvajal
Stowers Institute for Medical Research, Kansas City, MO, United States; University of Utah, Salt Lake City, UT, United States.
T
T
Piotrowski
D000818:Animals; D030801:Animals, Genetically Modified; D002454:Cell Differentiation / Q000235:genetics*; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D048430:Cell Shape; D006198:Hair Cells, Auditory / Q000648:ultrastructure*; D006801:Humans; D053403:Lateral Line System / Q000254:growth & development / Q000648:ultrastructure*; D018613:Microscopy, Confocal / Q000379:methods*; D009024:Morphogenesis / Q000235:genetics*; D012038:Regeneration; D015027:Zebrafish / Q000235:genetics
Collective migration; Confocal imaging; Hair cells; Primordium; Regeneration; Sensory system; Spatial analysis; Time lapse; Transgenic lines; Zebrafish embryo