Developmental and stress regulation on expression of a novel miRNA, Fan-miR73, and its target ABI5 in strawberry.
Abscisic acid (ABA) is a critical plant hormone for fruit ripening and adaptive stress responses in strawberry. Previous high-throughput sequencing results indicated that ABA-insensitive (ABI)5, an important transcription factor in the ABA signaling pathway, was a target for a novel microRNA (miRNA), Fan-miR73. In the present study, exogenous ABA treatment was found to accelerate fruit ripening through differentially regulating the transcripts of ABA metabolism and signal transduction related genes, including NCED1, PYR1, ABI1, and SnRK2.2. Expression of Fan-miR73 was down-regulated in response to exogenous ABA treatment in a dosage-dependent manner, which resulted in an accumulation of ABI5 transcripts in the ripening-accelerated fruits. In addition, both UV-B radiation and salinity stress reduced the transcript levels of Fan-miR73, whereas promoted ABI5 expression. Furthermore, high negative correlations between the transcriptional abundance of Fan-miR73 and ABI5 were observed during ripening and in response to stress stimuli. These results enriched the possible regulatory role of miRNA involved in the post-transcriptional modification of ABI5 during strawberry ripening, as well as responses to environmental stresses.
Zhejiang University, College of Biosystems Engineering and Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Hangzhou, 310058, People's Republic of China.
Dongdong
D
Li
Zhejiang University, College of Biosystems Engineering and Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Hangzhou, 310058, People's Republic of China.
Wangshu
W
Mou
Zhejiang University, College of Biosystems Engineering and Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Hangzhou, 310058, People's Republic of China.
Zisheng
Z
Luo
Zhejiang University, College of Biosystems Engineering and Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Hangzhou, 310058, People's Republic of China.
Li
L
Li
Zhejiang University, College of Biosystems Engineering and Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Hangzhou, 310058, People's Republic of China.
Jarukitt
J
Limwachiranon
Zhejiang University, College of Biosystems Engineering and Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Hangzhou, 310058, People's Republic of China.
Linchun
L
Mao
Zhejiang University, College of Biosystems Engineering and Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Hangzhou, 310058, People's Republic of China.
Tiejin
T
Ying
D000040:Abscisic Acid / Q000378:metabolism*; D031985:Fragaria / Q000235:genetics* / Q000254:growth & development; D005638:Fruit / Q000235:genetics; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D035683:MicroRNAs / Q000235:genetics*; D020929:Mitogen-Activated Protein Kinase Kinases / Q000235:genetics*; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics; D015398:Signal Transduction; D014157:Transcription Factors / Q000378:metabolism; D014466:Ultraviolet Rays
D035683:MicroRNAs; D010937:Plant Growth Regulators; D010940:Plant Proteins; D018749:RNA, Plant; D014157:Transcription Factors; D000040:Abscisic Acid; D020929:Mitogen-Activated Protein Kinase Kinases