Validation of Reference Genes in mRNA Expression Analysis Applied to the Study of Asthma.
The quantitative Polymerase Chain Reaction is the most used technique for the study of gene expression. To correct putative experimental errors of this technique is necessary normalizing the expression results of the gene of interest with the obtained for reference genes. Here, we describe an example of the process to select reference genes. In this particular case, we select reference genes for expression studies in the peripheral blood mononuclear cells of asthmatic patients.
Department of Clinical Biochemistry, University Hospital of Salamanca, Salamanca, Spain.|Salamanca Institute for Biomedical Research (IBSAL), Salamanca, Spain.
Ignacio San
IS
Segundo-Val
Salamanca Institute for Biomedical Research (IBSAL), Salamanca, Spain. catsof@usal.es.|Department of Microbiology and Genetics, University of Salamanca, Salamanca, 37007, Spain. catsof@usal.es.
Catalina S
CS
Sanz-Lozano
D000465:Algorithms; D001249:Asthma / Q000097:blood / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods / Q000592:standards; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis* / Q000097:blood; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods / Q000592:standards*; D012015:Reference Standards
D012333:RNA, Messenger
Constitutive gene; Expression analysis; Normalization; Reference gene; Validation reference gene; gene; qPCR