Crosstalk Between Human Monocytic U937 Cells and Gingival Fibroblasts in Coculturally Enhanced Matrix Metalloproteinase-2 Expression.
Matrix metalloproteinases (MMPs) play a key role in inflammatory periodontal disease. Synergistically enhanced MMP-2 expression in a coculture of human gingival fibroblasts (HGFs) and human monocytic U937 cells was observed. Crosstalk between these two cells via the extracellular matrix metalloproteinase inducer (EMMPRIN) was demonstrated.



Enzyme levels of MMP-2 in HGFs and direct coculture with U937 were examined by zymography. MMP-2 and EMMPRIN expressions of HGFs and U937 were determined in coculture and conditioned cultures (using supernatants from HGF- or U937-conditioned medium). The crosstalk was evaluated by EMMPRIN extrasupplement and EMMPRIN inhibition, through pretreatment of U937 with cyclosporine-A.



Direct coculturing of HGFs and U937 enhanced MMP-2 enzyme level and mRNA expression. Coculturing also increased membranous EMMPRIN expression of U937, but not from HGFs. In conditioned cultures, mRNA expression of MMP-2 increased in HGFs which received U937-conditioned medium. Increased MMP-2 was not observed in U937 with HGF-conditioned medium, although mRNA expression of EMMPRIN increased. Enhanced MMP-2 was observed after administration of exogenous EMMPRIN in HGFs; however, reduced MMP-2 enzyme level was noted if EMMPRIN of cocultured U937 was inhibited.



In the coculture of HGFs and U937, upregulated EMMPRIN expression in U937, which may be triggered by HGFs, can enhance MMP-2 expression in HGFs. Crosstalk between HGFs and U937 involving MMP-2 from HGFs was proposed; EMMPRIN from U937 may play a particular role.
Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan, Republic of China.|Department of Periodontology, National Defense Medical Center and Tri-Service General Hospital, Taipei, Taiwan, Republic of China.
Po-Jan
PJ
Kuo
Department of Periodontology, National Defense Medical Center and Tri-Service General Hospital, Taipei, Taiwan, Republic of China.
Hsiao-Lun
HL
Lin
Department of Periodontology, National Defense Medical Center and Tri-Service General Hospital, Taipei, Taiwan, Republic of China.
Chi-Yu
CY
Lin
Department of Periodontology, National Defense Medical Center and Tri-Service General Hospital, Taipei, Taiwan, Republic of China.|Institute of Cancer Biology and Drug Discovery, Taipei Medical University, Taipei, Taiwan, Republic of China.
Yu-Tang
YT
Chin
Department of Periodontology, National Defense Medical Center and Tri-Service General Hospital, Taipei, Taiwan, Republic of China.
Hsiao-Pei
HP
Tu
Department of Periodontology, Cardinal Tien Hospital, New Taipei City, Taiwan, Republic of China.
Tat-Ming
TM
Lai
Department of Periodontology, National Defense Medical Center and Tri-Service General Hospital, Taipei, Taiwan, Republic of China.
Hsien-Chung
HC
Chiu
Department of Periodontology, National Defense Medical Center and Tri-Service General Hospital, Taipei, Taiwan, Republic of China.
Earl
E
Fu
D051926:Basigin / Q000502:physiology*; D005347:Fibroblasts; D005881:Gingiva / Q000378:metabolism*; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020298:U937 Cells
D051926:Basigin; D020778:Matrix Metalloproteinase 2
Antigens, CD147; U937 cells; coculture techniques; fibroblasts; gingiva; matrix metalloproteinase 2