Analysis of dysregulated long non-coding RNA expressions in glioblastoma cells.
Long non coding RNAs (lncRNAs) are associated with various biological roles such as embryogenesis, stem cell biology, cellular development and present specific tissue expression profiles. Aberrant expression of lncRNAs are thought to play a critical role in the progression and development of various cancer types, including gliomas. Glioblastomas (GBM) are common and malignant primary brain tumours. Brain cancer stem cells (BCSC) are isolated from both low and high-grade tumours in adults and children, by cell fraction which express neuronal stem cell surface marker CD133. The purpose of this study was to investigate the expression profiles of lncRNAs in brain tumour cells and determine its potential biological function. For this purpose, U118MG-U87MG; GBM stem cell series were used. Human parental brain cancer cells were included as the control group; the expressions of disease related human lncRNA profiles were studied by LightCycler 480 real-time PCR. Expression profiles of 83 lncRNA genes were analyzed for a significant dysregulation, compared to the control cells. Among lncRNAs, 51 lncRNA genes down-regulated, while 8 lncRNA genes were up-regulated. PCAT-1 (-2.36), MEG3 (-5.34), HOTAIR (-2.48) lncRNAs showed low expression in glioblastoma compared to the human (parental) brain cancer stem cells, indicating their role as tumour suppressor genes on gliomas. As a result, significant changes for anti-cancer gene expressions were detected with disease-related human lncRNA array plates. Identification of novel target genes may lead to promising developments in human brain cancer treatment.
Vocational School of Health Services, Near East University, North Cyprus. Electronic address: tbalcii@gmail.com.
Tugce
T
Balci
Faculty of Medicine, Department of Medical Biology, Ege University, Bornova, Izmir, Turkey.
Sunde
S
Yilmaz Susluer
Faculty of Medicine, Department of Medical Biology, Ege University, Bornova, Izmir, Turkey.
Cagla
C
Kayabasi
Faculty of Medicine, Department of Medical Biology, Ege University, Bornova, Izmir, Turkey.
Besra
B
Ozmen Yelken
Faculty of Medicine, Department of Medical Biology, Ege University, Bornova, Izmir, Turkey.
Cigir
C
Biray Avci
Faculty of Medicine, Department of Medical Biology, Ege University, Bornova, Izmir, Turkey.
Cumhur
C
Gunduz
D000071916:AC133 Antigen / Q000235:genetics / Q000378:metabolism; D000328:Adult; D001921:Brain / Q000378:metabolism / Q000473:pathology; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002648:Child; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D053263:Gene Regulatory Networks; D005909:Glioblastoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000071916:AC133 Antigen; C000606023:PROM1 protein, human; D062085:RNA, Long Noncoding; D012333:RNA, Messenger
Glioblastoma; Human parental brain cancer stem cell; Long non coding RNAs