Enhanced and selective permeability of gold nanoparticles functionalized with cell penetrating peptide derived from maurocalcine animal toxin.
Functionalization of gold nanoparticles (GNPs) is suitable for many applications such as biomedical imaging, clinical diagnosis, and targeted delivery by conjugating cell-penetrating peptides (CPPs). Here, we investigated intracellular uptake of GNP conjugated to MCaUF1-9(Ala) , a CPP derived from maurocalcine (MCa) animal toxin, and compared it with TAT functionalized GNP. Peptide conjugated GNP was characterized using UV-Visible spectroscopy, dynamic light scattering, zeta potential, and transmission electron microscopy. Uptake of MCaUF1-9(Ala) and TAT functionalized GNPs was evaluated in three cell lines, HeLa, MDA-MB-231, and A431, using dark field imaging and atomic absorption spectroscopy. According to peptide sequences and type of cells different cell penetrating activity was observed. Peptide functionalized GNP had little effect on cell viability and respect to net charge difference between peptide, showed interesting selectivity against three cell types. Peptide conjugated to GNPs displayed higher uptake than bare GNPs in the all cell lines except HeLa cell with lowest internalization. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2693-2700, 2016.
Department of Nanobiotechnology, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran.
Sedigheh
S
Khamehchian
Department of Nanobiotechnology, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran.
Maryam
M
Nikkhah
Department of Biotechnology, Razi Vaccine and Serum Research Institute, Karaj, Iran.
Rasool
R
Madani
Department of Nanobiotechnology, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran. saman_h@modares.ac.ir.
Saman
S
Hosseinkhani
D000595:Amino Acid Sequence; D045744:Cell Line, Tumor; D002470:Cell Survival; D057846:Cell-Penetrating Peptides / Q000032:analysis / Q000737:chemistry* / Q000493:pharmacokinetics; D016503:Drug Delivery Systems; D006046:Gold / Q000032:analysis / Q000737:chemistry* / Q000493:pharmacokinetics; D006801:Humans; D053768:Metal Nanoparticles / Q000032:analysis / Q000737:chemistry* / Q000648:ultrastructure; D010539:Permeability; D012604:Scorpion Venoms / Q000032:analysis / Q000737:chemistry* / Q000493:pharmacokinetics
D057846:Cell-Penetrating Peptides; D012604:Scorpion Venoms; C406406:maurocalcine; D006046:Gold
cell penetration peptide; cytotoxicity; gold nanoparticle; nanomaterial