A rapidly photo-activatable light-up fluorescent nucleoside and its application in DNA base variation sensing.
A new DNA building block (d(Tet)U) bearing a tetrazole and allyloxy group at N-phenyl ring linked through an aminopropynyl linker to the 5-position of 2'-deoxyuridine was synthesized. The modified DNA can be lit up via a photoinduced intramolecular tetrazole-alkene cycloaddition reaction, but quenched when the fully-matched double strand is formed. This conspicuous difference in fluorescence could open a door for DNA single nucleotide polymorphism (SNP) typing.
College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei 430072, P. R. China. xzhou@whu.edu.cn dyh@whu.edu.cn.
Zhiyong
Z
He
College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei 430072, P. R. China. xzhou@whu.edu.cn dyh@whu.edu.cn.
Yuqi
Y
Chen
College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei 430072, P. R. China. xzhou@whu.edu.cn dyh@whu.edu.cn.
Yafen
Y
Wang
College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei 430072, P. R. China. xzhou@whu.edu.cn dyh@whu.edu.cn.
Jiaqi
J
Wang
College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei 430072, P. R. China. xzhou@whu.edu.cn dyh@whu.edu.cn.
Jing
J
Mo
College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei 430072, P. R. China. xzhou@whu.edu.cn dyh@whu.edu.cn.
Boshi
B
Fu
College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei 430072, P. R. China. xzhou@whu.edu.cn dyh@whu.edu.cn.
Zijing
Z
Wang
College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei 430072, P. R. China. xzhou@whu.edu.cn dyh@whu.edu.cn.
Yuhao
Y
Du
College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei 430072, P. R. China. xzhou@whu.edu.cn dyh@whu.edu.cn.
Xiang
X
Zhou
D000475:Alkenes / Q000737:chemistry; D061565:Cycloaddition Reaction; D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D005456:Fluorescent Dyes / Q000737:chemistry*; D008027:Light; D009705:Nucleosides / Q000737:chemistry*; D020641:Polymorphism, Single Nucleotide; D013777:Tetrazoles / Q000737:chemistry
D000475:Alkenes; D005456:Fluorescent Dyes; D009705:Nucleosides; D013777:Tetrazoles; C045574:1H-tetrazole; D004247:DNA