Possible Involvement of MYB44-Mediated Stomatal Regulation in Systemic Resistance Induced by Penicillium simplicissimum GP17-2 in Arabidopsis.
The plant growth-promoting fungus (PGPF), Penicillium simplicissimum GP17-2 (GP17-2), induces systemic resistance against Pseudomonas syringae pv. tomato DC3000 (Pst) in Arabidopsis thaliana. The molecular mechanisms underlying induced systemic resistance (ISR) by GP17-2 were investigated in the present study. Microscopic observations revealed that stomatal reopening by Pst was restricted by elicitation with the culture filtrate (CF) from GP17-2. A gene expression analysis of MYB44, which enhances abscisic acid signaling and consequently closes stomata, revealed that the gene was activated by CF. CF-elicited myb44 mutant plants failed to restrict stomatal reopening and showed lower resistance to Pst than wild-type plants. These results indicate that stomatal resistance by GP17-2 is mediated by the gene activation of MYB44. We herein revealed that the MYB44-mediated prevention of penetration through the stomata is one of the components responsible for GP17-2-elicited ISR.
The Graduate School of Agricultural Science, Gifu University.
Ayaka
A
Hieno
Hushna Ara
HA
Naznin
Mitsuro
M
Hyakumachi
Mieko
M
Higuchi-Takeuchi
Minami
M
Matsui
Yoshiharu Y
YY
Yamamoto
D017360:Arabidopsis / Q000235:genetics / Q000276:immunology* / Q000382:microbiology* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D010407:Penicillium / Q000254:growth & development*; D054046:Plant Stomata / Q000502:physiology*; D044224:Pseudomonas syringae / Q000276:immunology; D014157:Transcription Factors / Q000378:metabolism*
D029681:Arabidopsis Proteins; C529221:At5g67300 protein, Arabidopsis; D014157:Transcription Factors