Production of N-Acetylglucosamine Deacetylase by Vibrio cholerae Non-O1.
Vibrio cholerae non-O1 (1148 A) produced β-N-acetylglucosamini-dase and N-acetylglucosamine (GlcNAc) deacetylase intracellularly when grown in chitin or GlcNAc containing medium. It also secreted chitinase only in the chitin-containing medium. The partially purified GlcNAc deacetylase deacetylated GlcNAc but not chitin oligosaccharides, the dimer to hexamer of GlcNAc. We also detected the reaction product by capillary electrophoresis.
a Government Industrial Research Institute, Osaka, Midorigaoka , Ikeda, Osaka 563 , Japan.
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Yamano
a Government Industrial Research Institute, Osaka, Midorigaoka , Ikeda, Osaka 563 , Japan.
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Fujishima
a Government Industrial Research Institute, Osaka, Midorigaoka , Ikeda, Osaka 563 , Japan.
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Miwatani
a Government Industrial Research Institute, Osaka, Midorigaoka , Ikeda, Osaka 563 , Japan.
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Yaku
a Government Industrial Research Institute, Osaka, Midorigaoka , Ikeda, Osaka 563 , Japan.
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Tanaka
a Government Industrial Research Institute, Osaka, Midorigaoka , Ikeda, Osaka 563 , Japan.|b Research Institute of Microbial Diseases, Osaka University , Yamadaoka, Suita, Osaka 565 , Japan.
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Arita