Dynamic Surfaces for the Study of Mesenchymal Stem Cell Growth through Adhesion Regulation.
Out of their niche environment, adult stem cells, such as mesenchymal stem cells (MSCs), spontaneously differentiate. This makes both studying these important regenerative cells and growing large numbers of stem cells for clinical use challenging. Traditional cell culture techniques have fallen short of meeting this challenge, but materials science offers hope. In this study, we have used emerging rules of managing adhesion/cytoskeletal balance to prolong MSC cultures by fabricating controllable nanoscale cell interfaces using immobilized peptides that may be enzymatically activated to change their function. The surfaces can be altered (activated) at will to tip adhesion/cytoskeletal balance and initiate differentiation, hence better informing biological mechanisms of stem cell growth. Tools that are able to investigate the stem cell phenotype are important. While large phenotypical differences, such as the difference between an adipocyte and an osteoblast, are now better understood, the far more subtle differences between fibroblasts and MSCs are much harder to dissect. The development of technologies able to dynamically navigate small differences in adhesion are critical in the race to provide regenerative strategies using stem cells.
Centre for Cell Engineering, Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, Joseph Black Building, University of Glasgow , Glasgow G12 8QQ, Scotland, U.K.
Jemma N
JN
Roberts
Department of Pure & Applied Chemistry, WestCHEM , Thomas Graham Building, 295 Cathedral Street, Glasgow G1 1XL, Scotland, U.K.
Jugal Kishore
JK
Sahoo
Centre for Cell Engineering, Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, Joseph Black Building, University of Glasgow , Glasgow G12 8QQ, Scotland, U.K.
Laura E
LE
McNamara
Glasgow Polyomics Facility, Translational Cancer Research Centre, University of Glasgow Garscube Campus, Switchback Road, Glasgow G61 1QH, Scotland, U.K.
Karl V
KV
Burgess
Centre for Cell Engineering, Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, Joseph Black Building, University of Glasgow , Glasgow G12 8QQ, Scotland, U.K.
Jingli
J
Yang
Centre for Cell Engineering, Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, Joseph Black Building, University of Glasgow , Glasgow G12 8QQ, Scotland, U.K.
Enateri V
EV
Alakpa
Centre for Cell Engineering, Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, Joseph Black Building, University of Glasgow , Glasgow G12 8QQ, Scotland, U.K.
Hilary J
HJ
Anderson
Centre for Cell Engineering, Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, Joseph Black Building, University of Glasgow , Glasgow G12 8QQ, Scotland, U.K.
Jake
J
Hay
Centre for Cell Engineering, Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, Joseph Black Building, University of Glasgow , Glasgow G12 8QQ, Scotland, U.K.
Lesley-Anne
LA
Turner
Centre for Cell Engineering, Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, Joseph Black Building, University of Glasgow , Glasgow G12 8QQ, Scotland, U.K.
Stephen J
SJ
Yarwood
School of Pharmacy, University of Nottingham , Boots Science Building, University Park, Nottingham NG7 2RD, U.K.|National Physical Laboratory , Teddington, Middlesex TW11 0LW, U.K.
Mischa
M
Zelzer
Bone & Joint Research Group, Centre for Human Development, Stem Cells and Regeneration, Institute of Developmental Sciences, University of Southampton , Southampton SO16 6YD, U.K.
Richard O C
RO
Oreffo
Department of Pure & Applied Chemistry, WestCHEM , Thomas Graham Building, 295 Cathedral Street, Glasgow G1 1XL, Scotland, U.K.|Advanced Science Research Center (ASRC), City University of New York , New York, New York 10031, United States.|Department of Chemistry and Biochemistry, City University of New York-Hunter College , 695 Park Avenue, New York, New York 10065, United States.
Rein V
RV
Ulijn
Centre for Cell Engineering, Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, Joseph Black Building, University of Glasgow , Glasgow G12 8QQ, Scotland, U.K.
Matthew J
MJ
Dalby
D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D059630:Mesenchymal Stem Cells; D036103:Nanotechnology; D010006:Osteoblasts
dynamic cell/material interface; mesenchymal stem cell; metabolomics; stem cell growth