Standards for Cell Line Authentication and Beyond.
Different genomic technologies have been applied to cell line authentication, but only one method (short tandem repeat [STR] profiling) has been the subject of a comprehensive and definitive standard (ASN-0002). Here we discuss the power of this document and why standards such as this are so critical for establishing the consensus technical criteria and practices that can enable progress in the fields of research that use cell lines. We also examine other methods that could be used for authentication and discuss how a combination of methods could be used in a holistic fashion to assess various critical aspects of the quality of cell lines.
Biosystems and Biomaterials Division, The National Institute of Standards and Technology, Gaithersburg, Maryland, United States of America.
Jamie L
0000-0001-5616-4029
JL
Almeida
Biosystems and Biomaterials Division, The National Institute of Standards and Technology, Gaithersburg, Maryland, United States of America.
Kenneth D
KD
Cole
Biosystems and Biomaterials Division, The National Institute of Standards and Technology, Gaithersburg, Maryland, United States of America.
Anne L
AL
Plant
D000818:Animals; D002460:Cell Line; D058893:DNA Barcoding, Taxonomic / Q000379:methods / Q000592:standards; D020869:Gene Expression Profiling / Q000379:methods* / Q000592:standards; D060005:Genotyping Techniques / Q000379:methods* / Q000592:standards; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D012015:Reference Standards; D015203:Reproducibility of Results