Quantitative detection of viable helminth ova from raw wastewater, human feces, and environmental soil samples using novel PMA-qPCR methods.
In this study, we have evaluated the efficacy of propidium monoazide quantitative polymerase chain reaction (PMA-qPCR) to differentiate between viable and non-viable Ancylostoma caninum ova. The newly developed method was validated using raw wastewater seeded with known numbers of A. caninum ova. Results of this study confirmed that PMA-qPCR has resulted in average of 88 % reduction (P < 0.05) in gene copy numbers for 50 % viable +50 % non-viable when compared with 100 % viable ova. A reduction of 100 % in gene copies was observed for 100 % non-viable ova when compared with 100 % viable ova. Similar reductions (79-80 %) in gene copies were observed for A. caninum ova-seeded raw wastewater samples (n = 18) collected from wastewater treatment plants (WWTPs) A and B. The newly developed PMA-qPCR method was applied to determine the viable ova of different helminths (A. caninum, A. duodenale, Necator americanus and Ascaris lumbricoides) in raw wastewater, human fecal and soil samples. None of the unseeded wastewater samples were positive for the above-mentioned helminths. N. americanus and A. lumbricoides ova were found in unseeded human fecal and soil samples. For the unseeded human fecal samples (1 g), an average gene copy concentration obtained from qPCR and PMA-qPCR was found to be similar (6.8 × 10(5) ± 6.4 × 10(5) and 6.3 × 10(5) ± 4.7 × 10(5)) indicating the presence of viable N. americanus ova. Among the 24 unseeded soil samples tested, only one was positive for A. lumbricoides. The mean gene copy concentration in the positively identified soil sample was 1.0 × 10(5) ± 1.5 × 10(4) (determined by qPCR) compared to 4.9 × 10(4) ± 3.7 × 10(3) (determined by PMA-qPCR). The newly developed PMA-qPCR methods were able to detect viable helminth ova from wastewater and soil samples and could be adapted for health risk assessment.
CSIRO Land and Water, Ecosciences Precinct, 41 Boggo Road, GPO Box 2583, Brisbane, QLD, 4102, Australia. pradep033@yahoo.com.|School of Public Health, The University of Queensland, Herston, QLD, 4006, Australia. pradep033@yahoo.com.
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Gyawali
CSIRO Land and Water, Ecosciences Precinct, 41 Boggo Road, GPO Box 2583, Brisbane, QLD, 4102, Australia.
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Ahmed
CSIRO Land and Water, Ecosciences Precinct, 41 Boggo Road, GPO Box 2583, Brisbane, QLD, 4102, Australia.|School of Public Health, The University of Queensland, Herston, QLD, 4006, Australia.
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JP
Sidhu
Research School of Population Health, College of Medicine, Biology and Environment, The Australian National University, Linnaeus Way, ACT, 2601, Australia.
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SV
Nery
Research School of Population Health, College of Medicine, Biology and Environment, The Australian National University, Linnaeus Way, ACT, 2601, Australia.
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Clements
Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC, 3052, Australia.
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Traub
Clinical Tropical Medicine Laboratory, QIMR Berghofer Medical Research Institute, Herston, QLD, 4006, Australia.|School of Medicine, The University of Queensland, Herston, QLD, 4006, Australia.
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JS
McCarthy
Clinical Tropical Medicine Laboratory, QIMR Berghofer Medical Research Institute, Herston, QLD, 4006, Australia.
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Llewellyn
School of Public Health, The University of Queensland, Herston, QLD, 4006, Australia.
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Jagals
CSIRO Land and Water, Ecosciences Precinct, 41 Boggo Road, GPO Box 2583, Brisbane, QLD, 4102, Australia.|School of Public Health, The University of Queensland, Herston, QLD, 4006, Australia.
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Toze
D000818:Animals; D001386:Azides; D004784:Environmental Monitoring / Q000379:methods*; D005243:Feces / Q000469:parasitology*; D006376:Helminths / Q000502:physiology*; D006801:Humans; D010063:Ovum; D011419:Propidium / Q000031:analogs & derivatives; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012987:Soil / Q000469:parasitology*; D062065:Wastewater / Q000469:parasitology*
D001386:Azides; D011419:Propidium; D012987:Soil; D062065:Wastewater; C533957:propidium monoazide
Hookworm ova; PMA-qPCR; Public health; Viability; Wastewater