Antimicrobial activity of poly(vinyl alcohol)-poly(acrylic acid) electrospun nanofibers.
Electrospun nanofibers were prepared from blends of poly(acrylic acid) (PAA) and poly(vinyl alcohol) (PVA). The fibers were stabilized by heat curing at 140°C via anhydride and ketone formation and crosslinking esterification. The antimicrobial effect was assessed using strains of Escherichia coli and Staphylococcus aureus by tracking their capacity to form colonies and their metabolic impairment upon contact with PAA/PVA membranes. Membranes containing >35wt.% PAA displayed significant antibacterial activity, which was particularly high for the gram-positive S. aureus. All membranes were negatively charged, with surface ζ-potential in the (-34.5)-(-45.6)mV range, but the electrostatic interaction with the negatively charged cells was not the reason for the antimicrobial effect. Neither pH reduction nor the passing of non-crosslinked polymers to the solution affected microbial growth. The antibacterial activity was attributed to the chelation of the divalent cations stabilizing the outer cell membrane. The effect on gram-positive bacteria was attributed to the destabilization of the peptidoglycan layer. The sequestration of divalent cations was demonstrated with experiments in which calcium and a chelating agent were added to the cultures in contact with membranes. The damage to bacterial cells was tracked by measuring their surface charge and the evolution of intracellular calcium during the early stages after contact with PAA/PVA membranes.
Department of Chemical Engineering, University of Alcalá, E-28871 Alcalá de Henares, Madrid, Spain.
Javier
J
Santiago-Morales
Department of Chemical Engineering, University of Alcalá, E-28871 Alcalá de Henares, Madrid, Spain.
Georgiana
G
Amariei
Department of Chemical Engineering, University of Alcalá, E-28871 Alcalá de Henares, Madrid, Spain; Advanced Study Institute of Madrid, IMDEA-Agua, Parque Científico Tecnológico, E-28805 Alcalá de Henares, Madrid, Spain.
Pedro
P
Letón
Department of Chemical Engineering, University of Alcalá, E-28871 Alcalá de Henares, Madrid, Spain; Advanced Study Institute of Madrid, IMDEA-Agua, Parque Científico Tecnológico, E-28805 Alcalá de Henares, Madrid, Spain. Electronic address: roberto.rosal@uah.es.
Roberto
R
Rosal
D000180:Acrylic Resins / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects*; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry*; D011108:Polymers / Q000008:administration & dosage / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects*
D000180:Acrylic Resins; D000900:Anti-Bacterial Agents; D011108:Polymers; C006903:carbopol 940; D011142:Polyvinyl Alcohol
Antimicrobial membranes; Electrospinning; Poly(acrylic acid); Poly(vinyl alcohol)