[Inclusion Bodies are Formed in SFTSV-infected Human Macrophages].
The severe fever with thrombocytopenia syndrome virus (SFTSV) is a new member in the genus Phlebovirus of the family Bunyaviridae identified in China. The SFTSV is also the causative pathogen of an emerging infectious disease: severe fever with thrombocytopenia syndrome. Using immunofluorescent staining and confocal microscopy, the intracellular distribution of nucleocapsid protein (NP) in SFTSV-infected THP-1 cells was investigated with serial doses of SFTSV at different times after infection. Transmission electron microscopy was used to observe the ultrafine intracellular structure of SFTSV-infected THP-1 cells at different times after infection. SFTSV NP could form intracellular inclusion bodies in infected THP-1 cells. The association between NP-formed inclusion bodies and virus production was analyzed: the size of the inclusion body formed 3 days after infection was correlated with the viral load in supernatants collected 7 days after infection. These findings suggest that the inclusion bodies formed in SFTSV-infected THP-1 cells could be where the SFTSV uses host-cell proteins and intracellular organelles to produce new viral particles.
Cong
C
Jin
Jingdong
J
Song
Ying
Y
Han
Chuan
C
Li
Peihong
P
Qiu
Mifang
M
Liang
D002460:Cell Line; D002681:China; D006801:Humans; D007181:Inclusion Bodies, Viral / Q000648:ultrastructure / Q000821:virology*; D008264:Macrophages / Q000648:ultrastructure / Q000821:virology*; D010217:Phlebotomus Fever / Q000821:virology*; D016856:Phlebovirus / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D013921:Thrombocytopenia / Q000821:virology*