Piceatannol and Other Wine Stilbenes: A Pool of Inhibitors against α-Synuclein Aggregation and Cytotoxicity.
The aggregation of α-synuclein is one on the key pathogenic events in Parkinson's disease. In the present study, we investigated the inhibitory capacities of stilbenes against α-synuclein aggregation and toxicity. Thioflavin T fluorescence, transmission electronic microscopy, and SDS-PAGE analysis were performed to investigate the inhibitory effects of three stilbenes against α-synuclein aggregation: piceatannol, ampelopsin A, and isohopeaphenol. Lipid vesicle permeabilization assays were performed to screen stilbenes for protection against membrane damage induced by aggregated α-synuclein. The viability of PC12 cells was examined using an MTT assay to assess the preventive effects of stilbenes against α-synuclein-induced toxicity. Piceatannol inhibited the formation of α synuclein fibrils and was able to destabilize preformed filaments. It seems to induce the formation of small soluble complexes protecting membranes against α-synuclein-induced damage. Finally, piceatannol protected cells against α-synuclein-induced toxicity. The oligomers tested (ampelopsin A and hopeaphenol) were less active.
Universit&#233; de Bordeaux, ISVV, EA 4577 Oenologie, Facult&#233; de Pharmacie, MIB (GESVAB), Villenave d'Ornon 33882, France. temsamani.hamza.ht@gmail.com.|INRA, ISVV, USC 1366 Oenologie, Villenave d'Ornon 33882, France. temsamani.hamza.ht@gmail.com.
Hamza
H
Temsamani
Universit&#233; de Bordeaux, ISVV, EA 4577 Oenologie, Facult&#233; de Pharmacie, MIB (GESVAB), Villenave d'Ornon 33882, France. Stephanie.Krisa@u-bordeaux.fr.|INRA, ISVV, USC 1366 Oenologie, Villenave d'Ornon 33882, France. Stephanie.Krisa@u-bordeaux.fr.
Stéphanie
S
Krisa
CBMN-UMR 5248 CNRS, Universit&#233; de Bordeaux, IPB, All&#233;e Geoffroy St. Hilaire, Pessac 33600, France. m.decossas@cbmn.u-bordeaux.fr.
Marion
M
Decossas-Mendoza
CBMN-UMR 5248 CNRS, Universit&#233; de Bordeaux, IPB, All&#233;e Geoffroy St. Hilaire, Pessac 33600, France. o.lambert@cbmn.u-bordeaux.fr.
Olivier
O
Lambert
Universit&#233; de Bordeaux, ISVV, EA 4577 Oenologie, Facult&#233; de Pharmacie, MIB (GESVAB), Villenave d'Ornon 33882, France. jean-michel.merillon@u-bordeaux.fr.|INRA, ISVV, USC 1366 Oenologie, Villenave d'Ornon 33882, France. jean-michel.merillon@u-bordeaux.fr.
Jean-Michel
JM
Mérillon
Universit&#233; de Bordeaux, ISVV, EA 4577 Oenologie, Facult&#233; de Pharmacie, MIB (GESVAB), Villenave d'Ornon 33882, France. tristan.richard@u-bordeaux.fr.|INRA, ISVV, USC 1366 Oenologie, Villenave d'Ornon 33882, France. tristan.richard@u-bordeaux.fr.
Tristan
T
Richard
D000818:Animals; D052160:Benzothiazoles; D002470:Cell Survival / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D005419:Flavonoids / Q000494:pharmacology; D046529:Microscopy, Electron, Transmission; D016716:PC12 Cells; D010300:Parkinson Disease / Q000188:drug therapy; D010636:Phenols; D051381:Rats; D013267:Stilbenes / Q000494:pharmacology*; D013844:Thiazoles; D014920:Wine / Q000032:analysis*; D051844:alpha-Synuclein / Q000037:antagonists & inhibitors / Q000633:toxicity*
D052160:Benzothiazoles; D005419:Flavonoids; D010636:Phenols; D013267:Stilbenes; D013844:Thiazoles; D051844:alpha-Synuclein; C518233:hopeaphenol; C009462:thioflavin T; C106407:ampelopsin; C041525:3,3',4,5'-tetrahydroxystilbene
Parkinson’s disease; piceatannol; stilbene; α-synuclein