A replication-time-controlling sequence element in Schizosaccharomyces pombe.
Eukaryotic replication origins are highly variable in their activity and replication timing. The nature and role of cis-acting regulatory sequences that control chromosomal replication timing is not well defined. In the fission yeast, Schizosaccharomyces pombe, a 200-bp late-replication-enforcing element (LRE), has been shown to enforce late replication of ARS elements in plasmids. Here, we show that a short (133-bp) fragment of the LRE (shLRE) is required for causing late replication of adjoining origins in its native as well as in an ectopic early-replicating chromosomal location. Active from both sides of an early-replicating origin, the shLRE is a bona fide cis-acting regulatory element that imposes late replication timing in the chromosome.
Department of Biotechnology, Veer Bahadur Singh Purvanchal University, Jaunpur, UP, 222003, India.
Vishnu P
VP
Tripathi
Department of Biotechnology, Veer Bahadur Singh Purvanchal University, Jaunpur, UP, 222003, India. dddubey2003@gmail.com.
Dharani D
DD
Dubey
D001483:Base Sequence; D042522:DNA Replication Timing; D004271:DNA, Fungal / Q000235:genetics*; D015180:Electrophoresis, Gel, Two-Dimensional; D018741:Replication Origin; D012568:Schizosaccharomyces / Q000235:genetics*
D004271:DNA, Fungal
2D gel analysis; ARS elements; Fission yeast; Replication origins; Replication timing