Delayed progression of rabies transmitted by a vampire bat.
Here, we compared the growth kinetics, cell-to-cell spread, and virus internalization kinetics in N2a cells of RABV variants isolated from vampire bats (V-3), domestic dogs (V-2) and marmosets (V-M) as well as the clinical symptoms and mortality caused by these variants. The replication rate of V-3 was significantly higher than those of V-2 and V-M. However, the uptake and spread of these RABV variants into N2a cells were inversely proportional. Nevertheless, V-3 had longer incubation and evolution periods. Our results provide evidence that the clinical manifestations of infection with bat RABV variant occur at a later time when compared to what was observed with canine and marmoset rabies virus variants.
Laboratório de Diagnóstico da Raiva, Instituto Pasteur of São Paulo, São Paulo, SP, Brazil. ianasuly@gmail.com.
Iana Suly Santos
IS
Katz
Laboratório de Diagnóstico da Raiva, Instituto Pasteur of São Paulo, São Paulo, SP, Brazil.
Natalia Langenfeld
NL
Fuoco
Laboratório de Diagnóstico da Raiva, Instituto Pasteur of São Paulo, São Paulo, SP, Brazil.
Luciana Botelho
LB
Chaves
Laboratório de Diagnóstico da Raiva, Instituto Pasteur of São Paulo, São Paulo, SP, Brazil.
Adriana Candido
AC
Rodrigues
Laboratório de Imunogenética, Instituto Butantan of São Paulo, São Paulo, SP, Brazil.
Orlando Garcia
OG
Ribeiro
Laboratório de Diagnóstico da Raiva, Instituto Pasteur of São Paulo, São Paulo, SP, Brazil.
Karin Corrêa
KC
Scheffer
Laboratório de Diagnóstico da Raiva, Instituto Pasteur of São Paulo, São Paulo, SP, Brazil.
Karen Miyuki
KM
Asano
D000818:Animals; D000956:Antigens, Viral; D002144:Callithrix / Q000821:virology; D045744:Cell Line, Tumor; D002685:Chiroptera / Q000821:virology*; D004285:Dogs / Q000821:virology; D051379:Mice; D011818:Rabies / Q000473:pathology / Q000662:veterinary* / Q000821:virology; D011820:Rabies virus / Q000145:classification / Q000502:physiology*
D000956:Antigens, Viral
Bat virus; Dog virus; Marmoset virus; Rabies virus; Replication