Flavonoids from Anoectochilus annamensis and their Anti-inflammatory Activity.
One new flavonol diglycoside, 4',5-dihydroxy-3,3',7-trimethoxyflavone 4'-O-α-L-rhamnopyranosyl-(1 --> 6)-β-D-glucopyranoside (1), and two known compounds (2-3) were isolated from the methanolic extract of Anoectochilus annamensis Aver. aerial parts. The effects were evaluated of all isolated compounds (1-3) on LPS-induced production of the inflammatory mediator nitric oxide (NO) by RAW264.7 cells. 4',5-Dihydroxy-3,3',7-trimethoxyflavone (2) was the most active while the addition of a rutinoside at C4' (compound 1) decreased the inhibitory activity. This is the first report on the chemical composition and biological activity of A. annamensis.
Tran Minh
TM
Hoi
Tran Van
TV
Thai
Chu Thi Thu
CT
Ha
Ha Thi Van
HT
Anh
Phan Xuan Binh
PX
Minh
Nguyen Tien
NT
Dat
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification*; D051379:Mice; D029595:Orchidaceae / Q000737:chemistry*; D000067996:RAW 264.7 Cells
D000893:Anti-Inflammatory Agents; D005419:Flavonoids