A New Route of Fucoidan Immobilization on Low Density Polyethylene and Its Blood Compatibility and Anticoagulation Activity.
Beside biomaterials' bulk properties, their surface properties are equally important to control interfacial biocompatibility. However, due to the inadequate interaction with tissue, they may cause foreign body reaction. Moreover, surface induced thrombosis can occur when biomaterials are used for blood containing applications. Surface modification of the biomaterials can bring enhanced surface properties in biomedical applications. Sulfated polysaccharide coatings can be used to avoid surface induced thrombosis which may cause vascular occlusion (blocking the blood flow by blood clot), which results in serious health problems. Naturally occurring heparin is one of the sulfated polysaccharides most commonly used as an anticoagulant, but its long term usage causes hemorrhage. Marine sourced sulfated polysaccharide fucoidan is an alternative anticoagulant without the hemorrhage drawback. Heparin and fucoidan immobilization onto a low density polyethylene surface after functionalization by plasma has been studied. Surface energy was demonstrated by water contact angle test and chemical characterizations were carried out by Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. Surface morphology was monitored by scanning electron microscope and atomic force microscope. Finally, their anticoagulation activity was examined for prothrombin time (PT), activated partial thromboplastin time (aPTT), and thrombin time (TT).
Centre of Polymer Systems, Tomas Bata University in Zl&#237;n, Tr. Tomase Bati 5678, 760 01 Zl&#237;n, Czech Republic. kadirozaltin@hotmail.com.
Kadir
K
Ozaltin
Centre of Polymer Systems, Tomas Bata University in Zl&#237;n, Tr. Tomase Bati 5678, 760 01 Zl&#237;n, Czech Republic. lehocky@post.cz.
Marián
M
Lehocký
Centre of Polymer Systems, Tomas Bata University in Zl&#237;n, Tr. Tomase Bati 5678, 760 01 Zl&#237;n, Czech Republic. humpolicek@ft.utb.cz.
Petr
P
Humpolíček
Department of Hematology, Vsetin Hospital, Nemocnicni 955, 755 01 Vsetin, Czech Republic. pelkova@fhs.utb.cz.|Faculty of Humanities, Tomas Bata University in Zl&#237;n, Mostni 5139, 760 01 Zl&#237;n, Czech Republic. pelkova@fhs.utb.cz.
Jana
J
Pelková
Centre of Polymer Systems, Tomas Bata University in Zl&#237;n, Tr. Tomase Bati 5678, 760 01 Zl&#237;n, Czech Republic. saha@rektorat.utb.cz.
Petr
P
Sáha
D000925:Anticoagulants / Q000009:adverse effects / Q000737:chemistry* / Q000494:pharmacology; D001769:Blood / Q000187:drug effects; D006493:Heparin / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D020959:Polyethylene / Q000737:chemistry*; D011134:Polysaccharides / Q000009:adverse effects / Q000737:chemistry* / Q000494:pharmacology
D000925:Anticoagulants; D011134:Polysaccharides; D020959:Polyethylene; D006493:Heparin; C007789:fucoidan
anticoagulant; biomaterials; fucoidan; heparin; plasma treatment; thrombosis