Protective effects of polysaccharides from Psidium guajava leaves against oxidative stresses.
The aim of this study was to analyze antioxidant properties of a polysaccharide isolated from Psidium guajava leaves (PS-PGL) in vitro including its radical scavenging activities and protective effects against damage to cells as well as in vivo in zebrafish. The water extract of P. guajava leaves (WE-PGL) and PS-PGL showed strong radical scavenging effects in terms of 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl, and alkyl radical. Compared to WE-PGL, PS-PGL enhanced all scavenging activities and in particular strongly scavenged the hydroxyl radical (50% inhibitory concentration [IC50], 0.02mg/mL). In addition, PS-PGL exerted a protective effect against hydrogen peroxide-induced oxidative stress and against toxicity to Vero cells. Furthermore, in vivo experiments using zebrafish embryos indicated that treatment with hydrogen peroxide decreased the survival rate and heart-beating rate of zebrafish embryos, whereas these problems were reduced by PS-PGL treatment. Moreover, PS-PGL inhibited hydrogen peroxide-induced reactive oxygen species (ROS) production, lipid peroxidation, and cell death. Taken together, these results suggest that PS-PGL may be useful as a beneficial antioxidant material in the food and cosmetic industries.
Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
Seo-Young
SY
Kim
Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea.
Eun-A
EA
Kim
Jeju Aloe Agriculture Association Cooperation, Sehwa-ri, Pyosun-myeon, Seogwipo, Jeju-do 690-1, Republic of Korea.
Young-Sun
YS
Kim
Jeju Aloe Agriculture Association Cooperation, Sehwa-ri, Pyosun-myeon, Seogwipo, Jeju-do 690-1, Republic of Korea.
Seok-Kyu
SK
Yu
Department of Polymer Science and Engineering, Sunchon National University, 255 Jungang-ro, Sunchon, Jeonnam 540-742, Republic of Korea.
Changyong
C
Choi
Industry-Academic Cooperation Foundation, Jeju National University, Jeju 690-756, Republic of Korea.
Jung-Suk
JS
Lee
Department of Food Science and Biotechnology, Kunsan National University, Kunsan 573-701, Republic of Korea.
Yong-Tae
YT
Kim
Department of Polymer Science and Engineering, Sunchon National University, 255 Jungang-ro, Sunchon, Jeonnam 540-742, Republic of Korea. Electronic address: jwnah@sunchon.ac.kr.
Jae-Woon
JW
Nah
Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea. Electronic address: youjinj@jejunu.ac.kr.
You-Jin
YJ
Jeon
D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002522:Chlorocebus aethiops; D018384:Oxidative Stress / Q000187:drug effects*; D010088:Oxidoreductases / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D031647:Psidium / Q000737:chemistry*; D017382:Reactive Oxygen Species / Q000378:metabolism; D014709:Vero Cells
D000975:Antioxidants; D011134:Polysaccharides; D017382:Reactive Oxygen Species; D010088:Oxidoreductases
Antioxidant; Polysaccharides; Psidium guajava leaves