Transcriptional signatures of influenza A/H1N1-specific IgG memory-like B cell response in older individuals.
Studies suggest that the recall-based humoral immune responses to influenza A/H1N1 originates from activated memory B cells. The aim of this study was to identify baseline, early and late blood transcriptional signatures (in peripheral blood mononuclear cells/PBMCs) associated with memory B cell response following influenza vaccination.



We used pre- and post-vaccination mRNA-Seq transcriptional profiling on samples from 159 subjects (50-74years old) following receipt of seasonal trivalent influenza vaccine containing the A/California/7/2009/H1N1-like virus, and penalized regression modeling to identify associations with influenza A/H1N1-specific memory B cell ELISPOT response after vaccination.



Genesets and genes (p-value range 7.92E(-08) to 0.00018, q-value range 0.00019-0.039) demonstrating significant associations (of gene expression levels) with memory B cell response suggest the importance of metabolic (cholesterol and lipid metabolism-related), cell migration/adhesion, MAP kinase, NF-kB cell signaling (chemokine/cytokine signaling) and transcriptional regulation gene signatures in the development of memory B cell response after influenza vaccination.



Through an unbiased transcriptome-wide profiling approach, our study identified signatures of memory B cell response following influenza vaccination, highlighting the underappreciated role of metabolic changes (among the other immune function-related events) in the regulation of influenza vaccine-induced immune memory.
Mayo Clinic Vaccine Research Group, Mayo Clinic, Rochester, MN 55905, USA.
Iana H
IH
Haralambieva
Mayo Clinic Vaccine Research Group, Mayo Clinic, Rochester, MN 55905, USA.
Inna G
IG
Ovsyannikova
Mayo Clinic Vaccine Research Group, Mayo Clinic, Rochester, MN 55905, USA.
Richard B
RB
Kennedy
Division of Biomedical Statistics and Informatics, Department of Health Science Research, Mayo Clinic, Rochester, MN 55905, USA.
Michael T
MT
Zimmermann
Division of Biomedical Statistics and Informatics, Department of Health Science Research, Mayo Clinic, Rochester, MN 55905, USA.
Diane E
DE
Grill
Division of Biomedical Statistics and Informatics, Department of Health Science Research, Mayo Clinic, Rochester, MN 55905, USA.
Ann L
AL
Oberg
Mayo Clinic Vaccine Research Group, Mayo Clinic, Rochester, MN 55905, USA. Electronic address: poland.gregory@mayo.edu.
Gregory A
GA
Poland
D000914:Antibodies, Viral / Q000097:blood; D001402:B-Lymphocytes / Q000276:immunology*; D058501:Enzyme-Linked Immunospot Assay; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D056724:Immunity, Humoral; D007156:Immunologic Memory; D053118:Influenza A Virus, H1N1 Subtype; D007252:Influenza Vaccines / Q000276:immunology* / Q000627:therapeutic use; D007251:Influenza, Human / Q000517:prevention & control; D008297:Male; D008875:Middle Aged; D059467:Transcriptome; D055815:Young Adult
D000914:Antibodies, Viral; D007252:Influenza Vaccines
Aging; B-lymphocytes; Enzyme-linked immunospot assay; Gene expression; Immunity, Humoral; Influenza A virus, H1N1 subtype; Influenza vaccines; Influenza, Human; RNA; Sequence analysis; Vaccination