Label-Free Detection of Glycan-Protein Interactions for Array Development by Surface-Enhanced Raman Spectroscopy (SERS).
A glyco-array platform has been developed, in which glycans are attached to plasmonic nanoparticles through strain-promoted azide-alkyne cycloaddition. Glycan-protein binding events can then be detected in a label-free manner employing surface-enhanced Raman spectroscopy (SERS). As proof of concept, we have analyzed the binding of Gal1, Gal3, and influenza hemagglutinins (HAs) to various glycans and demonstrated that binding partners can be identified with high confidence. The attraction of SERS for optical sensing is that it can provide unique spectral signatures for glycan-protein complexes, confirm identity through statistical validation, and minimizes false positive results common to indirect methods. Furthermore, SERS is very sensitive and has multiplexing capabilities thereby allowing the simultaneous detection of multiple analytes.
Complex Carbohydrate Research Center and Department of Chemistry The University of Georgia, 315 Riverbend Road, Athens, GA 30602 (USA).
Xiuru
X
Li
Department of Chemistry, The University of Georgia Athens, GA 30602 (USA).
Sharon J H
SJH
Martin
Complex Carbohydrate Research Center and Department of Chemistry The University of Georgia, 315 Riverbend Road, Athens, GA 30602 (USA).|Department of Chemistry, The University of Georgia Athens, GA 30602 (USA).
Zoeisha S
ZS
Chinoy
Complex Carbohydrate Research Center and Department of Chemistry The University of Georgia, 315 Riverbend Road, Athens, GA 30602 (USA).
Lin
L
Liu
Department of Chemistry, The University of Georgia Athens, GA 30602 (USA).
Brandon
B
Rittgers
Department of Chemistry, The University of Georgia Athens, GA 30602 (USA).|Department of Chemistry, The University of Alabama at Birmingham Birmingham, AL 35294 (USA).
Richard A
RA
Dluhy
Complex Carbohydrate Research Center and Department of Chemistry The University of Georgia, 315 Riverbend Road, Athens, GA 30602 (USA).|Department of Chemistry, The University of Georgia Athens, GA 30602 (USA).|Department of Chemical Biology and Drug Discovery Utrecht Institute for Pharmaceutical Sciences and Bijvoet Center for Biomolecular Research, Utrecht University Universiteitsweg 99, 3584 CG Utrecht (The Netherlands).
Geert-Jan
GJ
Boons
D053758:Nanoparticles / Q000737:chemistry*; D011134:Polysaccharides / Q000032:analysis* / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000379:methods*
D011134:Polysaccharides
Raman spectroscopy; galectin; hemagglutinin; oligosaccharides; proteins