In vitro fermentation of sulfated polysaccharides from E. prolifera and L. japonica by human fecal microbiota.
In vitro fermentation of the sulfated polysaccharides from seaweeds Enteromorpha prolifera and Laminaria japonica and their prebiotic effects on human fecal microbiota were investigated in this study. The sulfated polysaccharides were fermented in vitro for 48h by human fecal cultures. When 0.8g MWCOL (polysaccharides MWCO<30kD) from L. japonica was fermented, the pH in fecal cultures decreased from 6.5 to 5.1 and the levels of short chain fatty acids, such as acetic, butyric and lactic acids all significantly increased. After 48h fermentation, 0.8g MWCOL showed good effect on modulating the gut microflora balance, because the beneficial strains (Lactobacillus and Bifidobacterium) were both significantly higher than those in control group (p<0.05). As far as we know, this is the first report that consumption of sulfated polysaccharides from E. prolifera and L. japonica is beneficial to the ecosystem of the intestinal tract by increasing the populations of probiotics and short chain fatty acids. Furthermore, our reports indicated that molecular weight of sulfated polysaccharide from marine algae is related to its prebiotic effects.
School of Food Science and Engineering, Ocean University of China, Shandong 266003, China. Electronic address: kongqing@ouc.edu.cn.
Qing
Q
Kong
School of Food Science and Engineering, Ocean University of China, Shandong 266003, China.
Shiyuan
S
Dong
School of Food Science and Engineering, Ocean University of China, Shandong 266003, China.
Jian
J
Gao
School of Food Science and Engineering, Ocean University of China, Shandong 266003, China.
Chaoyu
C
Jiang
D001644:Bifidobacterium / Q000378:metabolism*; D005243:Feces / Q000382:microbiology*; D006801:Humans; D007778:Lactobacillus / Q000378:metabolism*; D007795:Laminaria / Q000737:chemistry*; D064307:Microbiota; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*
D011134:Polysaccharides
Enteromorpha prolifera; In vitro fermentation; Laminaria japonica