Arm Selection Preference of MicroRNA-193a Varies in Breast Cancer.
MicroRNAs (miRNAs) are short noncoding RNAs derived from the 3' and 5' ends of the same precursor. However, the biological function and mechanism of miRNA arm expression preference remain unclear in breast cancer. We found significant decreases in the expression levels of miR-193a-5p but no significant differences in those of miR-193a-3p in breast cancer. MiR-193a-3p suppressed breast cancer cell growth and migration and invasion abilities, whereas miR-193a-5p suppressed cell growth but did not influence cell motility. Furthermore, NLN and CCND1, PLAU, and SEPN1 were directly targeted by miR-193a-5p and miR-193a-3p, respectively, in breast cancer cells. The endogenous levels of miR-193a-5p and miR-193a-3p were significantly increased by transfecting breast cancer cells with the 3'UTR of their direct targets. Comprehensive analysis of The Cancer Genome Atlas database revealed significant differences in the arm expression preferences of several miRNAs between breast cancer and adjacent normal tissues. Our results collectively indicate that the arm expression preference phenomenon may be attributable to the target gene amount during breast cancer progression. The miRNA arm expression preference may be a means of modulating miRNA function, further complicating the mRNA regulatory network. Our findings provide a new insight into miRNA regulation and an application for breast cancer therapy.
Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.|Department of Chemical Biology, National Pingtung University of Education, Pingtung, Taiwan.
Kuo-Wang
KW
Tsai
Department of Radiation Oncology, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.
Chung-Man
CM
Leung
Department of Family Medicine, Zuoying Branch of Kaohsiung Armed Forces General Hospital, Kaohsiung, Taiwan.
Yi-Hao
YH
Lo
Molecular Medicine Research Center, Chang Gung University, Taoyuan, Taiwan.|Bioinformatics Center, Chang Gung University, Taoyuan, Taiwan.
Ting-Wen
TW
Chen
Genomics &Proteomics Core Laboratory, Department of medical research, Kaohsiung, Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan.
Wen-Ching
WC
Chan
Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.
Shou-Yu
SY
Yu
Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.
Ya-Ting
YT
Tu
Center For Geriatrics and Gerontology, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.
Hing-Chung
HC
Lam
Genomics &Proteomics Core Laboratory, Department of medical research, Kaohsiung, Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan.
Sung-Chou
SC
Li
Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.|Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan.
Luo-Ping
LP
Ger
Department of Radiation Oncology, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.|Department of Radiation Oncology, Tri-Service General Hospital, Taipei, Twiwan.
Wen-Shan
WS
Liu
Center For Geriatrics and Gerontology, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.|Department of Surgery, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan.
Hong-Tai
HT
Chang
D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D019938:Cyclin D1 / Q000235:genetics; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D008565:Membrane Proteins / Q000235:genetics; D035683:MicroRNAs / Q000096:biosynthesis / Q000145:classification / Q000235:genetics*; D009124:Muscle Proteins / Q000235:genetics; D009361:Neoplasm Invasiveness / Q000235:genetics* / Q000473:pathology; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D051140:Selenoproteins / Q000235:genetics
C530104:CCND1 protein, human; C546818:MIRN193 microRNA, human; D008565:Membrane Proteins; D035683:MicroRNAs; D009124:Muscle Proteins; C000625496:PLAU protein, human; D034741:RNA, Small Interfering; C435489:SELENON protein, human; D051140:Selenoproteins; D019938:Cyclin D1