[Association of genetic polymorphisms in HjV-BMPs-SMADs pathway of hepcidin regulation with the incidence of anemia in Chinese adolescent aged 12 -18 years old].
OBJECTIVE
To investigate the associations of the genetic polymorphisms in HjV-BMPs-SMADs pathway of hepcidin regulation with the incidence of anemia in Chinese adolescent.


METHODS
657 boarding school students aged 12 -18 were recruited by using cluster random sampling method from Chengdu Sichuan Province, Baicheng Jilin Province, Zhaoqing Guangdong Province, Puding Guizhou Province, Wenshang Shandong Province and Songyang Zhejiang Province. Cyanomethemoglobin determination test was used to measured the concentration of Hb, and then the subjects were classified by "Method for anemia screen" into corresponding groups. Magnetic heads for DNA extraction was selected, and 29 variants were genotyped by conducted by Sequenom MassArray. The Hb levels and anemia in different groups were compared with the genotype of each locus by chi-square test and t test aimed to study the associations between them.


RESULTS
The risk of homozygote of T on rs173 107 to anemia was 5.80 times higher than homozygote of G (P < 0.05, OR = 5.80, 95% CI 1.34 - 25.04), and the concentrations of H b were (143.7 ± 14.6) g/L and (145.6 ± 12.0) g/L, respectively. The risk of heterozygote on rs855791 to anemia was 4.00 times higher than homozygote of A (P < 0.05, OR = 4.00, 95% CI 1.35 - 11.82), and the concentrations of Hb were (144.2 ± 14.8) g/L and 145.8 ± 12.0 g/L, respectively. The risk of G allele carriers was 3.68 times higher than homozygote of A (P < 0.05, OR = 3.68, 95% CI 1.27 - 10.66), and the concentrations of Hb were (144.3 ± 14.4) g/L and ( 145.8 ± 12.0) g/L, respectively. The levels of Hb among groups of the two loci were shown no different (P > 0.05), but the lowering trends were observed in the homozygote of T on rs173107 and G allele carriers on rs855791 groups.


CONCLUSIONS
Variants rs173107 and rs855791 in HjV-BMPs-SMADs pathway are associate with anemia in Chinese adolescent.
Wei
W
Piao
Li
L
Wang
Ting
T
Zhang
Zhen
Z
Wang
Shangguan Shaofang
Jing
J
Sun
Junsheng
J
Huo
D000293:Adolescent; D000483:Alleles; D000740:Anemia / Q000235:genetics*; D044466:Asian People / Q000235:genetics; D019485:Bone Morphogenetic Proteins / Q000235:genetics*; D002648:Child; D002681:China; D005838:Genotype; D000071020:Hemochromatosis Protein; D006454:Hemoglobins / Q000032:analysis; D064451:Hepcidins / Q000378:metabolism*; D006579:Heterozygote; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006720:Homozygote; D006801:Humans; D015994:Incidence; D008565:Membrane Proteins / Q000235:genetics*; D011110:Polymorphism, Genetic; D051785:Smad Proteins / Q000235:genetics*
D019485:Bone Morphogenetic Proteins; C103673:HFE protein, human; D000071020:Hemochromatosis Protein; D006454:Hemoglobins; D064451:Hepcidins; D015395:Histocompatibility Antigens Class I; D008565:Membrane Proteins; D051785:Smad Proteins