Recent advances in whole cell biocatalysis techniques bridging from investigative to industrial scale.
Recent advances in biocatalysis have strongly boosted its recognition as a valuable addition to traditional chemical synthesis routes. As for any catalytic process, catalyst's costs and stabilities are of highest relevance for the economic application in chemical manufacturing. Employing biocatalysts as whole cells circumvents the need of cell lysis and enzyme purification and hence strongly cuts on cost. At the same time, residual cell wall components can shield the entrapped enzyme from potentially harmful surroundings and aid to enable applications far from natural enzymatic environments. Further advantages are the close proximity of reactants and catalysts as well as the inherent presence of expensive cofactors. Here, we review and comment on benefits and recent advances in whole cell biocatalysis.
IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, 52428 Jülich, Germany. Electronic address: J.Wachtmeister@fz-juelich.de.
Jochen
J
Wachtmeister
IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, 52428 Jülich, Germany. Electronic address: Do.Rother@fz-juelich.de.
Dörte
D
Rother
D000818:Animals; D055162:Biocatalysis; D035843:Biomedical Research / Q000379:methods* / Q000639:trends; D001709:Biotechnology / Q000379:methods / Q000639:trends; D004345:Drug Industry / Q000379:methods* / Q000639:trends*; D006801:Humans; D060847:Metabolic Engineering / Q000379:methods / Q000639:trends*