Determination of domoic acid in shellfish extracted by molecularly imprinted polymers.
A selective sample cleanup method using molecularly imprinted polymers was developed for the separation of domoic acid (a shellfish toxin) from shellfish samples. The molecularly imprinted polymers for domoic acid was prepared by emulsion polymerization using 1,3,5-pentanetricarboxylic acid as the template molecule, 4-vinyl pyridine as the functional monomer, ethylene glycol dimethacrylate as the crosslinker, and Span80/Tween-80 (1:1 v/v) as the composite emulsifiers. The molecularly imprinted polymer showed high affinity to domoic acid with a dissociation constant of 13.5 μg/mL and apparent maximum adsorption capacity of 1249 μg/g. They were used as a selective sorbent for the detection of domoic acid from seafood samples coupled with high-performance liquid chromatography. The detection limit of 0.17 μg/g was lower than the maximum level permitted by several authorities. The mean recoveries of domoic acid from clam samples were 93.0-98.7%. It was demonstrated that the proposed method could be applied to the determination of domoic acid from shellfish samples.
College of Food and Biological Engineering, Jimei University, Xiamen, China.|Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen, Fujian Province, China.
Zhengzhong
Z
Lin
College of Food and Biological Engineering, Jimei University, Xiamen, China.
Dan
D
Wang
College of Food and Biological Engineering, Jimei University, Xiamen, China.|Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen, Fujian Province, China.
Aihong
A
Peng
College of Food and Biological Engineering, Jimei University, Xiamen, China.|Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen, China.
Zhiyong
Z
Huang
College of Food and Biological Engineering, Jimei University, Xiamen, China.
Yuhui
Y
Lin
D000818:Animals; D049872:Bivalvia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D007608:Kainic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D057230:Limit of Detection; D008387:Marine Toxins / Q000032:analysis / Q000302:isolation & purification*; D054802:Molecular Imprinting; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D012758:Shellfish / Q000032:analysis*; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*
D008387:Marine Toxins; D011108:Polymers; C012301:domoic acid; D007608:Kainic Acid
Domoic acid; High-performance liquid chromatography; Molecularly imprinted polymers; Shellfish