Mechanism of Calcium Lactate Facilitating Phytic Acid Degradation in Soybean during Germination.
Calcium lactate facilitates the growth and phytic acid degradation of soybean sprouts, but the mechanism is unclear. In this study, calcium lactate (Ca) and calcium lactate with lanthanum chloride (Ca+La) were used to treat soybean sprouts to reveal the relevant mechanism. Results showed that the phytic acid content decreased and the availability of phosphorus increased under Ca treatment. This must be due to the enhancement of enzyme activity related to phytic acid degradation. In addition, the energy metabolism was accelerated by Ca treatment. The energy status and energy metabolism-associated enzyme activity also increased. However, the transmembrane transport of calcium was inhibited by La(3+) and concentrated in intercellular space or between the cell wall and cell membrane; thus, Ca+La treatment showed reverse results compared with those of Ca treatment. Interestingly, gene expression did not vary in accordance with their enzyme activity. These results demonstrated that calcium lactate increased the rate of phytic acid degradation by enhancing growth, phosphorus metabolism, and energy metabolism.
College of Food Science and Technology, Nanjing Agricultural University , Nanjing, Jiangsu 210095, People's Republic of China.
Qianru
Q
Hui
College of Food Science and Technology, Nanjing Agricultural University , Nanjing, Jiangsu 210095, People's Republic of China.
Runqiang
R
Yang
College of Food Science and Technology, Nanjing Agricultural University , Nanjing, Jiangsu 210095, People's Republic of China.
Chang
C
Shen
College of Food Science and Technology, Nanjing Agricultural University , Nanjing, Jiangsu 210095, People's Republic of China.
Yulin
Y
Zhou
College of Food Science and Technology, Nanjing Agricultural University , Nanjing, Jiangsu 210095, People's Republic of China.
Zhenxin
Z
Gu
D017610:Calcium Compounds / Q000032:analysis / Q000378:metabolism*; D018525:Germination; D007773:Lactates / Q000032:analysis / Q000378:metabolism*; D010833:Phytic Acid / Q000032:analysis / Q000378:metabolism*; D012639:Seeds / Q000737:chemistry / Q000254:growth & development* / Q000378:metabolism; D013025:Glycine max / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*
D017610:Calcium Compounds; D007773:Lactates; C110051:calcium lactate; D010833:Phytic Acid
calcium lactate; energy metabolism; phosphorus metabolism; phytic acid; soybean sprout