Algae as an electron donor promoting sulfate reduction for the bioremediation of acid rock drainage.
This study assessed bioremediation of acid rock drainage in simulated permeable reactive barriers (PRB) using algae, Chlorella sorokiniana, as the sole electron donor for sulfate-reducing bacteria. Lipid extracted algae (LEA), the residues of biodiesel production, were compared with whole cell algae (WCA) as an electron donor to promote sulfate-reducing activity. Inoculated columns containing anaerobic granular sludge were fed a synthetic medium containing H2SO4 and Cu(2+). Sulfate, sulfide, Cu(2+) and pH were monitored throughout the experiment of 123d. Cu recovered in the column packing at the end of the experiment was evaluated using sequential extraction. Both WCA and LEA promoted 80% of sulfate removal (12.7mg SO4(2-) d(-1)) enabling near complete Cu removal (>99.5%) and alkalinity generation raising the effluent pH to 6.5. No noteworthy sulfate reduction, alkalinity formation and Cu(2+) removal were observed in the endogenous control. In algae amended-columns, Cu(2+) was precipitated with biogenic H2S produced by sulfate reduction. Formation of CuS was evidenced by sequential extraction and X-ray diffraction. LEA and WCA provided similar levels of electron donor based on the COD balance. The results demonstrate an innovative passive remediation system using residual algae biomass from the biodiesel industry.
Department of Chemical and Environmental Engineering, The University of Arizona, P.O. Box 210011, Tucson, AZ, USA.
Pedro
P
Ayala-Parra
Department of Chemical and Environmental Engineering, The University of Arizona, P.O. Box 210011, Tucson, AZ, USA.
Reyes
R
Sierra-Alvarez
Department of Chemical and Environmental Engineering, The University of Arizona, P.O. Box 210011, Tucson, AZ, USA. Electronic address: jimfield@email.arizona.edu.
Jim A
JA
Field
D001673:Biodegradation, Environmental; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors; D002708:Chlorella / Q000737:chemistry* / Q000378:metabolism; D003300:Copper / Q000032:analysis*; D004583:Electrons; D006863:Hydrogen-Ion Concentration; D008962:Models, Theoretical; D010084:Oxidation-Reduction; D013431:Sulfates / Q000737:chemistry* / Q000378:metabolism; D013440:Sulfides / Q000737:chemistry; D016999:Sulfur-Reducing Bacteria / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000032:analysis*; D018508:Water Purification / Q000379:methods*
D013431:Sulfates; D013440:Sulfides; D014874:Water Pollutants, Chemical; D003300:Copper
Acid mine drainage; Algae waste; Biodiesel; Heavy metal; Permeable reactive barrier