AcMNPV-BmK IT improves the progeny virus production via baculovirus GP64 envelope fusion protein.
OBJECTIVE
To analyze the mechanisms underlying the impact of recombinant Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV)-mediated BmK IT expression on the function of baculovirus GP64 envelope fusion protein and progeny virus production.


RESULTS
Viral propagation assay indicated that overexpression GP64 could promote replication of AcMNPV. AcMNPV-mediated expression of BmK IT also promoted replication of AcMNPV. Immunofluorescence analysis showed BmK IT, which was regulated by very early promoter IE1 in AcMNPV, could make the GP64 protein move to the cytomembrane soon after transfection. BmK IT, which is regulated by P10 protein promoter (P10) and polyhedrosis promoter (PH), could promote the expression of GP64.


CONCLUSIONS
BmK IT, regulated by very early promoter IE1, P10 protein promoter (P10) and PH, accelerated the expression of GP64 protein, promoted its early cytomembrane localization and then triggered virus budding and progeny virus production.
Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, 030006, People's Republic of China. yjfu@sxu.edu.cn.
Yuejun
http://orcid.org/0000-0002-9575-7019
Y
Fu
Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, 030006, People's Republic of China.
Yanyan
Y
Miao
University of Miami Miller School of Medicine, Miami, FL, 33136, USA.
Shuhua
S
Zheng
Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, 030006, People's Republic of China.
Jun
J
Du
Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, 030006, People's Republic of China.
Aihua
A
Liang
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000502:physiology*; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012604:Scorpion Venoms / Q000235:genetics / Q000378:metabolism*; D061987:Sf9 Cells; D018411:Spodoptera / Q000821:virology*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000378:metabolism; D014760:Viral Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D010948:Viral Plaque Assay; D014779:Virus Replication
C509305:Bmk IT toxin, Buthus martensii Karsch; D011994:Recombinant Proteins; D012604:Scorpion Venoms; D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins
Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV); Baculovirus; Buthus martensii Karsch insect toxin (BmK IT); GP64 protein; Insecticidal protein; Promoter; Regulation mechanism