Mycobacterium tuberculosis Contaminant Risk on Bone Marrow Aspiration Material from Iliac Bone Patients with Active Tuberculous Spondylitis.
There was a concern on Mycobacterium tuberculosis spreading to the bone marrow, when it was applied on tuberculous spine infection. This research aimed to study the probability of using autologous bone marrow as a source of mesenchymal stem cell for patients with tuberculous spondylitis. As many as nine patients with tuberculous spondylitis were used as samples. During the procedure, the vertebral lesion material and iliac bone marrow aspirates were obtained for acid fast staining, bacteria culture, and PCR (polymerase chain reaction) tests for Mycobacterium tuberculosis at the Clinical Microbiology Laboratory of Faculty of Medicine Universitas Indonesia. This research showed that there was a relationship between diagnostic confirmation of tuberculous spondylitis based on the PCR test and bacterial culture on the solid vertebral lesion material with the PCR test and bacterial culture from the bone marrow aspirates. If the diagnostic confirmation concluded positive results, then there was a higher probability that there would be a positive result for the bone marrow aspirates, so that it was not recommended to use autologous bone marrow as a source of mesenchymal stem cell for patients with tuberculous spondylitis unless the PCR and culture examination of the bone marrow showed a negative result.
Department of Orthopaedic and Traumatology, Faculty of Medicine, University of Indonesia, Cipto Mangunkusumo Hospital, Jakarta 10320, Indonesia.
Ahmad Jabir
AJ
Rahyussalim
Stem Cell Integrated Medical Service Unit, Cipto Mangunkusumo Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta 0320, Indonesia.
Tri
T
Kurniawati
Department of Clinical Microbiology, Faculty of Medicine, University of Indonesia, Jakarta 10320, Indonesia.
Andriansjah
A
Rukmana
D000328:Adult; D001707:Biopsy, Needle / Q000379:methods; D001853:Bone Marrow / Q000382:microbiology* / Q000473:pathology*; D005260:Female; D006801:Humans; D007085:Ilium / Q000382:microbiology / Q000473:pathology*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000382:microbiology / Q000473:pathology; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D013048:Specimen Handling / Q000379:methods; D014399:Tuberculosis, Spinal / Q000382:microbiology* / Q000473:pathology*