Hydroethanolic extract of Baccharis trimera promotes gastroprotection and healing of acute and chronic gastric ulcers induced by ethanol and acetic acid.
Ethanol is a psychoactive substance highly consumed around the world whose health problems include gastric lesions. Baccharis trimera is used in folk medicine for the treatment of gastrointestinal disorders. However, few studies have evaluated its biological and toxic effects. To validate the popular use of B. trimera and elucidate its possible antiulcerogenic and cytotoxic mechanisms, a hydroethanolic extract of B. trimera (HEBT) was evaluated in models of gastric lesions. Rats and mice were used to evaluate the protective and antiulcerogenic effects of HEBT on gastric lesions induced by ethanol, acetic acid, and chronic ethanol consumption. The effects of HEBT were also evaluated in a pylorus ligature model and on gastrointestinal motility. The LD50 of HEBT in mice was additionally estimated. HEBT was analyzed by nuclear magnetic resonance, and a high-performance liquid chromatography fingerprint analysis was performed. Oral HEBT administration significantly reduced the lesion area and the oxidative stress induced by acute and chronic ethanol consumption. However, HEBT did not protect against gastric wall mucus depletion and did not alter gastric secretory volume, pH, or total acidity in the pylorus ligature model. Histologically, HEBT accelerated the healing of chronic gastric ulcers in rats, reflected by contractions of the ulcer base. Flavonoids and caffeoylquinic acids were detected in HEBT, which likely contributed to the therapeutic efficacy of HEBT, preventing or reversing ethanol- and acetic acid-induced ulcers, respectively. HEBT antiulcerogenic activity may be partially attributable to the inhibition of free radical generation and subsequent prevention of lipid peroxidation. Our results indicate that HEBT has both gastroprotective and curative activity in animal models, with no toxicity.
Biological Science Sector, Department of Pharmacology, Federal University of Paraná (UFPR), Centro Politécnico, Cx. P. 19031, Curitiba, Paraná, 81531-980, Brazil.
Francislaine Aparecida
FA
Dos Reis Lívero
Biological Science Sector, Department of Pharmacology, Federal University of Paraná (UFPR), Centro Politécnico, Cx. P. 19031, Curitiba, Paraná, 81531-980, Brazil.
Luisa Mota
LM
da Silva
Biological Science Sector, Department of Pharmacology, Federal University of Paraná (UFPR), Centro Politécnico, Cx. P. 19031, Curitiba, Paraná, 81531-980, Brazil.
Daniele Maria
DM
Ferreira
Biological Science Sector, Department of Pharmacology, Federal University of Paraná (UFPR), Centro Politécnico, Cx. P. 19031, Curitiba, Paraná, 81531-980, Brazil.
Larissa Favaretto
LF
Galuppo
Biological Science Sector, Department of Pharmacology, Federal University of Paraná (UFPR), Centro Politécnico, Cx. P. 19031, Curitiba, Paraná, 81531-980, Brazil.
Debora Gasparin
DG
Borato
Laboratory of Toxicology and Pharmacology, Paranaense University, Umuarama, PR, Brazil.
Thiago Bruno Lima
TB
Prando
Laboratory of Toxicology and Pharmacology, Paranaense University, Umuarama, PR, Brazil.
Emerson Luiz Botelho
EL
Lourenço
Department of Chemistry, Federal University of Paraná, Curitiba, PR, Brazil.
Regiane Lauriano Batista
RL
Strapasson
Department of Chemistry, Federal University of Paraná, Curitiba, PR, Brazil.
Maria Élida Alves
MÉ
Stefanello
Biological Science Sector, Department of Pharmacology, Federal University of Paraná (UFPR), Centro Politécnico, Cx. P. 19031, Curitiba, Paraná, 81531-980, Brazil.
Maria Fernanda de Paula
MF
Werner
Biological Science Sector, Department of Pharmacology, Federal University of Paraná (UFPR), Centro Politécnico, Cx. P. 19031, Curitiba, Paraná, 81531-980, Brazil. aleacco@ufpr.br.
Alexandra
A
Acco
D019342:Acetic Acid; D000818:Animals; D000897:Anti-Ulcer Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D000975:Antioxidants / Q000494:pharmacology; D031175:Baccharis / Q000737:chemistry; D019610:Cytoprotection; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000737:chemistry*; D005260:Female; D005746:Gastric Emptying / Q000187:drug effects; D005753:Gastric Mucosa / Q000378:metabolism; D005769:Gastrointestinal Motility / Q000187:drug effects; D007928:Lethal Dose 50; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D010946:Plants, Medicinal; D017208:Rats, Wistar; D012997:Solvents / Q000737:chemistry*; D013270:Stomach / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology; D013276:Stomach Ulcer / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*
D000897:Anti-Ulcer Agents; D000975:Antioxidants; D010936:Plant Extracts; D012997:Solvents; D000431:Ethanol; D019342:Acetic Acid
Antiulcerogenic; Baccharis trimera; Ethanol; Gastric ulcer; Oxidative stress