pH-responsive drug release from functionalized electrospun poly(caprolactone) scaffolds under simulated in vivo environment.
The difference in the tumor environment from the normal healthy tissue can be therapeutically exploited to develop new strategies for controlled and site-specific drug delivery. In the present study, a continuous flow system is designed to represent the in vivo environment of a tumor tissue and drug release is studied at different pH that represents normal tissue pH, tumor tissue pH, and stomach pH. The results obtained from these experiments were translated to a human embryonic kidney cell culture system and the effect of drug released from these functionalized PCL scaffolds on cell viability was studied. A significant decrease in cell viability was observed with the doxorubicin hydrochloride concentration that would be released at acidic pH, either present as a result of tumor extracellular environment or could be achieved via fabrication of a composite scaffold with a polyvinyl alcohol hydrogel containing acid. In the end, a study using zebrafish as an animal model is also undertaken in order to study the drug release from the scaffolds in vivo.
a Biomedical Engineering and Biotechnology Program , University of Massachusetts Dartmouth , Dartmouth , MA , USA.
Manisha
M
Jassal
a Biomedical Engineering and Biotechnology Program , University of Massachusetts Dartmouth , Dartmouth , MA , USA.
Vijay P
VP
Boominathan
b Department of Bioengineering , University of Massachusetts Dartmouth , Dartmouth , MA , USA.
Tracie
T
Ferreira
c Department of Civil and Environmental Engineering , University of Massachusetts Dartmouth , Dartmouth , MA , USA.
Sukalyan
S
Sengupta
d Department of Mechanical Engineering , University of Massachusetts Dartmouth , Dartmouth , MA , USA.
Sankha
S
Bhowmick
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology; D002470:Cell Survival; D004317:Doxorubicin / Q000737:chemistry / Q000494:pharmacology; D004337:Drug Carriers; D065546:Drug Liberation; D057809:HEK293 Cells; D006801:Humans; D020100:Hydrogels; D006863:Hydrogen-Ion Concentration; D057139:Nanofibers / Q000737:chemistry; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry; D013499:Surface Properties; D054457:Tissue Scaffolds / Q000737:chemistry*; D015027:Zebrafish
D000970:Antineoplastic Agents; D004337:Drug Carriers; D020100:Hydrogels; D011091:Polyesters; C016240:polycaprolactone; D004317:Doxorubicin; D011142:Polyvinyl Alcohol
Poly(caprolactone); composite scaffolds; continuous flow; doxorubicin hydrochloride; functionalization; in vivo study; pH-controlled delivery