Facile synthesis of magnetic molecularly imprinted polymer: Perphenazine template and its application in urine and plasma analysis.
Synthesis of magnetic iron oxide nanoparticles and its surface modification with methacrylic acid (MAA) was performed simultaneously by adding Fe(2+)/Fe(3+) to an alkaline MAA solution under nitrogen atmosphere. MAA coated magnetite (Fe3O4@MAA) has abundant reactive double bonds on the surface that can initiate polymerization. Magnetic molecularly imprinted polymers (MMIPs) were synthesized through distillation-precipitation polymerization of MAA as monomer, perphenazine (PPZ) as template, and ethylene glycol di-methacrylate (EGDMA) as cross linker on Fe3O4@MAA, with concise control of experimental conditions in about 90min. The produced super paramagnetic MMIPs can be separated from the solution in the presence of external magnetic field in less than 1min. Characterizations of the synthesized particles were performed by electron microscopes, thermo-gravimetric analysis (TGA), vibrating sample magnetometer (VSM), Fourier transform infrared (FT-IR) spectroscopy, and BET. The data showed that Fe3O4@MAA was well encapsulated in the polymer shell. The MMIPs showed high porosity. Moreover, MMIPs were used for rapid pre-concentration and separation of PPZ in human plasma and urine without any dilution and pretreatments using high performance liquid chromatography equipped with a photo diode array detector (HPLC-PDA). The calibration curve in urine and plasma has shown the same slope as the external calibration curve. Linear range of 20-5000ngmL(-1), and a detection limit of 5.3ngmL(-1) was obtained. The results showed 97.92% recovery along with the relative standard deviation of 6.07% (n=6) for 1μgmL(-1) PPZ. Pre-concentration factor was 13. The MMIPs adsorbed PPZ in 1min and then desorbed it by MeOH:HOAc in 2min.
Nanotechnology Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Mehdi
M
Safdarian
Nanotechnology Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Medicinal Chemistry Department, Faculty of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Electronic address: zramezani@ajums.ac.ir.
Zahra
Z
Ramezani
Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Ata A
AA
Ghadiri
D000327:Adsorption; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000592:standards; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008689:Methacrylates / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D054802:Molecular Imprinting; D010546:Perphenazine / Q000032:analysis* / Q000097:blood / Q000652:urine; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry
D058185:Magnetite Nanoparticles; D008689:Methacrylates; D011108:Polymers; C008384:methacrylic acid; D010546:Perphenazine
Magnetic molecular imprinted polymer; Nanoparticles; Perphenazine; Phenothiazines; Polymerization