A Case of Melanoacanthoma: Immunohistochemical Staining Using VECTOR® NovaRED™ to Distinguish Melanocytes from the Cutaneous Pigment.
Melanoacanthoma is a rare benign mixed tumor of both keratinocytes and melanocytes. Although some authors said that it is a rare variant of seborrheic keratosis, it has clinical and histological features distinct from seborrheic keratosis. It has large dendritic melanin-laden melanocytes throughout all levels of epidermis showing a disruption of melanin transfer from the melanocytes to neighboring keratinocytes. However, it is difficult to distinguish melanocytes clearly from cutaneous pigment in immunohistochemical stain with usually used brown chromogen. We used chromogen with brick-red indicator product (VECTOR® NovaRED™) in S-100 and melan-A immunohistochemical staining to distinguish melanocytes from melanin laden keratinocytes. We suggest that the immunohistochemical staining using this novel chromogen may be useful in the diagnosis of melanoacanthoma.
Department of Dermatology, College of Medicine, Korea University, Seoul, Korea.
Jae Eun
JE
Choi
Department of Dermatology, College of Medicine, Korea University, Seoul, Korea.
Eui Jong
EJ
Bae
Department of Pathology, College of Medicine, Korea University, Seoul, Korea.
Ae Ree
AR
Kim
Department of Dermatology, College of Medicine, Korea University, Seoul, Korea.
Sang Wook
SW
Son
Department of Dermatology, College of Medicine, Korea University, Seoul, Korea.
Hae Jun
HJ
Song
Department of Dermatology, College of Medicine, Korea University, Seoul, Korea.
Chil Hwan
CH
Oh
Immunohistochemistry; Melanoacanthoma; Seborrheic keratosis; VECTOR® NovaRED™