Influence of Physiological Stress on Nutrient Stoichiometry in Larval Amphibians.
Exposure to environmental stressors alters animal phenotypes as well as nutrient metabolism, assimilation, and excretion. While stress-induced shifts in nutrient processes are known to alter organismal carbon (C) and nitrogen (N) stoichiometry, there has been little exploration of how environmental factors influence phosphorous (P). A better understanding of how P cycling varies with animal physiological state may provide insight into across-scale processes, because P is essential to animal function and ecological processes such as production and decomposition. We tested the effects of predator stress and exogenous glucocorticoids on C∶N∶P stoichiometry of larval amphibians. Glucocorticoids altered nutrient stoichiometry, apparently by modulating ossification and renal function. This reduced whole-body P and significantly increased N∶P. Additionally, elevated glucocorticoids caused a long-term reduction in P excretion. This reduction may reflect an initial unmeasured loss of P that glucocorticoids induce over acute timescales. In contrast, exposure to predator cues had no effect on larval C∶N∶P stoichiometry, which highlights that different stressors have varied effects on the endocrine stress response. Predation, in particular, is ubiquitous in the environment; thus, larvae responding to predators have conserved mechanisms that likely prevent or minimize physiological disruption. These results demonstrate the differing physiological roles of N and P, distinct nutrient demands associated with amphibian metamorphosis, and the contrasting effects that different environmental factors have on the physiological stress response. Our results also suggest that anthropogenic changes to the environment that induce chronic stress in amphibians could affect the biogeochemistry of nutrient-poor environments where they may act as keystone species.
Lucas J
LJ
Kirschman
Savhannah
S
Haslett
Kelley A
KA
Fritz
Matt R
MR
Whiles
Robin W
RW
Warne
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology; D005502:Food; D006854:Hydrocortisone / Q000494:pharmacology; D007814:Larva / Q000502:physiology; D010710:Phosphates / Q000378:metabolism; D011235:Predatory Behavior; D011898:Ranidae / Q000502:physiology*; D013312:Stress, Physiological
D010710:Phosphates; D006854:Hydrocortisone
carbon; development; metamorphosis; nitrogen; phosphorus