A fast and low-cost genotyping method for hepatitis B virus based on pattern recognition in point-of-care settings.
A fast and low-cost method for HBV genotyping especially for genotypes A, B, C and D was developed and tested. A classifier was used to detect and analyze a one-step immunoassay lateral flow strip functionalized with genotype-specific monoclonal antibodies (mAbs) on multiple capture lines in the form of pattern recognition for point-of-care (POC) diagnostics. The fluorescent signals from the capture lines and the background of the strip were collected via multiple optical channels in parallel. A digital HBV genotyping model, whose inputs are the fluorescent signals and outputs are a group of genotype-specific digital binary codes (0/1), was developed based on the HBV genotyping strategy. Meanwhile, a companion decoding table was established to cover all possible pairing cases between the states of a group of genotype-specific digital binary codes and the HBV genotyping results. A logical analyzing module was constructed to process the detected signals in parallel without program control, and its outputs were used to drive a set of LED indicators, which determine the HBV genotype. Comparing to the nucleic acid analysis to HBV viruses, much faster HBV genotyping with significantly lower cost can be obtained with the developed method.
College of Information Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
Xianbo
X
Qiu
National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Xiamen University, Xiamen 361005, China.|Xiamen Innodx Biotech Company, Ltd., Xiamen 361026, China.
Liuwei
L
Song
College of Information Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
Shuo
S
Yang
College of Information Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
Meng
M
Guo
National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Xiamen University, Xiamen 361005, China.
Quan
Q
Yuan
National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Xiamen University, Xiamen 361005, China.
Shengxiang
S
Ge
National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Xiamen University, Xiamen 361005, China.
Xiaoping
X
Min
National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Xiamen University, Xiamen 361005, China.
Ningshao
N
Xia
D015331:Cohort Studies; D005838:Genotype; D006515:Hepatitis B virus / Q000235:genetics*; D019694:Hepatitis B, Chronic / Q000097:blood / Q000175:diagnosis / Q000821:virology; D006801:Humans; D019095:Point-of-Care Systems