Plasma miR-187* is a potential biomarker for oral carcinoma.
OBJECTIVE
Oral squamous cell carcinoma (OSCC) is prevalent worldwide, and survival in OSCC has not improved significantly in the past decades. MicroRNAs (miRNAs) have an important regulatory role in oral carcinogenesis. This study investigated the functional and clinical implications of miR-187* in OSCC pathogenesis.


METHODS
Expression of miR-187* in OSCC tissues and patient plasma was assayed using quantitative RT-PCR. The diagnostic power was specified using receiver operator curve analysis. The phenotypic influence of miR-187* in OSCC cells was delineated using exogenous expression.


RESULTS
miR-187* was upregulated in OSCC tissue relative to control mucosa. Overexpression of miR-187* enhanced the oncogenic phenotype of OSCC cells, including cell migration and anchorage-independent colony formation. Plasma miR-187* levels could be used to distinguish patients from controls with a separating power of 0.73. Patients showing a reduction in plasma miR-187* after tumor resection had a better prognosis.


CONCLUSIONS
miR-187* plays oncogenic roles in oral carcinogenesis. Plasma miR-187* could be validated as a marker of OSCC for diagnostic uses.


CONCLUSIONS
This research implied that plasma miR-187* was a diagnostic marker for patients with OSCC, and plasma miR-187* level could be a prognostic factor for OSCC patients who received ablation surgery.
Department of Oral and Maxillofacial Surgery, MacKay Memorial Hospital, Taipei, Taiwan.|MacKay Medical College, Taipei, Taiwan.|Department of Medical Research, MacKay Memorial Hospital, Taipei, Taiwan.|Institute of Oral Biology, School of Dentistry, National Yang-Ming University, No. 155, Li-Nong St., Sec.2, Taipei, Taiwan, 112.
Chung-Ji
CJ
Liu
Department of Oral and Maxillofacial Surgery, MacKay Memorial Hospital, Taipei, Taiwan.
Jiun-Sheng
JS
Lin
Department of Medical Research, MacKay Memorial Hospital, Taipei, Taiwan.
Hui-Wen
HW
Cheng
Institute of Oral Biology, School of Dentistry, National Yang-Ming University, No. 155, Li-Nong St., Sec.2, Taipei, Taiwan, 112.
Ya-Hui
YH
Hsu
Department of Oral and Maxillofacial Surgery, MacKay Memorial Hospital, Taipei, Taiwan.
Chieh-Yuan
CY
Cheng
Department of Stomatology, Taipei Veterans General Hospital, Taipei, Taiwan. sclin@ym.edu.tw.|Institute of Oral Biology, School of Dentistry, National Yang-Ming University, No. 155, Li-Nong St., Sec.2, Taipei, Taiwan, 112. sclin@ym.edu.tw.
Shu-Chun
SC
Lin
D015415:Biomarkers / Q000097:blood; D002294:Carcinoma, Squamous Cell / Q000097:blood* / Q000473:pathology*; D016022:Case-Control Studies; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000097:blood* / Q000473:pathology*; D009367:Neoplasm Staging; D010641:Phenotype; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D015854:Up-Regulation
D015415:Biomarkers; C571843:MIRN187 microRNA, human; D035683:MicroRNAs
Carcinoma; MicroRNA; Mouth; Plasma; miR-187*