Simultaneous Detection and Prevalence of Allergens in Anisakis Species Isolated from Marine Fishes.
This study was conducted to develop a multiplex reverse transcription (RT) PCR for the detection of Anisakis allergens and to investigate the relationship between allergen profiles and anisakid larvae isolated from Scomber japonicus, Trichiurus lepturus, and Conger myriaster in Korea. The species of Anisakis was determined using Anisakis pegreffii-specific PCR and restriction fragment length polymorphism analysis. The prevalence and profiles of five Ani s allergens were examined by multiplex RTPCR. A. pegreffii and Anisakis typica accounted for 97.1 and 2.9%, respectively, of the 140 larvae examined. In A. pegreffii, allergen prevalence was 41.2% for Ani s 1, 72.1% for Ani s 2, 69.9% for Ani s 3, 86.7% for Ani s 4, and 93.4% for Ani s 5. Most A. pegreffii larvae had multiple allergen profiles, and 80.7% of A. pegreffii carried both Ani s 4 and Ani s 5, which are heat-resistant allergens. Fifty-two to 65% of A. pegreffii isolated from S. japonicus and C. myriaster carried all five Ani s allergens.
Department of Food and Nutrition, School of Food Science and Technology, Chung-Ang University, 4726 Seodongdae-ro, Ansung-si, Kyounggi-d, 456-756, Republic of Korea.
Woo Joo
WJ
Lee
Department of Food and Nutrition, School of Food Science and Technology, Chung-Ang University, 4726 Seodongdae-ro, Ansung-si, Kyounggi-d, 456-756, Republic of Korea.
Dong Joo
DJ
Seo
Department of Food and Nutrition, School of Food Science and Technology, Chung-Ang University, 4726 Seodongdae-ro, Ansung-si, Kyounggi-d, 456-756, Republic of Korea.
Hyejin
H
Oh
Department of Food and Nutrition, School of Food Science and Technology, Chung-Ang University, 4726 Seodongdae-ro, Ansung-si, Kyounggi-d, 456-756, Republic of Korea.
Su Been
SB
Jeon
Department of Food and Nutrition, School of Food Science and Technology, Chung-Ang University, 4726 Seodongdae-ro, Ansung-si, Kyounggi-d, 456-756, Republic of Korea.
Day
D
Jung
Department of Food and Nutrition, School of Food Science and Technology, Chung-Ang University, 4726 Seodongdae-ro, Ansung-si, Kyounggi-d, 456-756, Republic of Korea. cchoi@cau.ac.kr.
Changsun
C
Choi
D000485:Allergens; D000818:Animals; D017163:Anisakis; D005393:Fish Diseases; D005399:Fishes / Q000469:parasitology*; D007814:Larva; D015995:Prevalence; D056910:Republic of Korea
D000485:Allergens