Sulfur mustard causes oxidants/antioxidants imbalance through the overexpression of free radical producing-related genes in human mustard lungs.
The aim of this study is to analyze oxidative stress (OS) and changes in expression of reactive oxygen species (ROS) producing-related genes in mustard lungs. Human lung biopsies provided from controls (n=5) and sulfur mustard (SM)-exposed patients (n=6). Changes in expression of dual oxidases (DUOXs), aldehyde oxidase 1 (AOX1), thyroid peroxidase (TPO), myeloperoxidase (MPO) and eosinophil Peroxidase (EPO) were measured using RT(2) Profiler(™) PCR Array. OS was evaluated by determining bronchoalveolar lavage fluids (BALF) levels of total antioxidant capacity (TAC) and malondialdehyde (MDA). Higher TAC value was observed in BALF of controls compared with patients (0.138±0.02683μmol/l vs 0.0942±0.01793μmol/l), whereas a significant increase in MDA concentration was found in patients (0.486±0.04615 nmol/l vs 0.6467±0.05922 nmol/l). All ROS producing-related genes were overexpressed in the order AOX1>MPO>DUOX2>DUOX1>TPO>EPO. Upregulation of these genes may be a reason for overproduction of ROS, oxidants/antioxidants imbalance, OS and respiratory failures in mustard lungs.
Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. Electronic address: tahmasb@hotmail.com.
Eisa
E
Tahmasbpour Marzony
Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Amir
A
Nejad-Moghadam
Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Mostafa
M
Ghanei
Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. Electronic address: yunespanahi@yahoo.com.
Yunes
Y
Panahi
D042931:Aldehyde Oxidase / Q000235:genetics; D000975:Antioxidants / Q000378:metabolism*; D000068337:Armed Conflicts; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D016022:Case-Control Studies; D002619:Chemical Warfare Agents / Q000633:toxicity*; D000074623:Dual Oxidases; D047209:Eosinophil Peroxidase / Q000235:genetics; D053263:Gene Regulatory Networks / Q000187:drug effects*; D006801:Humans; D007453:Iodide Peroxidase / Q000235:genetics; D008168:Lung / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009151:Mustard Gas / Q000633:toxicity*; D019255:NADPH Oxidases / Q000235:genetics; D018384:Oxidative Stress / Q000187:drug effects / Q000235:genetics; D009195:Peroxidase / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D012129:Respiratory Function Tests
D000975:Antioxidants; D002619:Chemical Warfare Agents; D017382:Reactive Oxygen Species; D000074623:Dual Oxidases; D047209:Eosinophil Peroxidase; D009195:Peroxidase; D007453:Iodide Peroxidase; C526436:AOX1 protein, human; D042931:Aldehyde Oxidase; D019255:NADPH Oxidases; C431513:DUOX1 protein, human; C431515:DUOX2 protein, human; D009151:Mustard Gas
Lung tissue; Oxidants/antioxidants imbalance; Oxidative stress; ROS producing-related genes; Sulfur mustard