Association between Placental Lesions, Cytokines and Angiogenic Factors in Pregnant Women with Preeclampsia.
Preeclampsia (PE) is considered the leading cause of maternal and perinatal morbidity and mortality. The placenta seems to play an essential role in this disease, probably due to factors involved in its formation and development. The present study aimed to investigate the association between placental lesions, cytokines and angiogenic factors in pregnant women with preeclampsia (PE). We evaluated 20 normotensive pregnant women, 40 with early-onset PE and 80 with late-onset PE. Placental samples were analyzed for histopathology, immunohistochemistry and determination of granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-10 (IL-10), transforming growth factor-beta 1 (TGF-β1), tumor necrosis factor-alpha (TNF-α), placental growth factor (PlGF), vascular endothelial growth factor (VEGF), fms-like tyrosine-kinase-1 (Flt-1) and endoglin (Eng) levels. Higher percentages of increased syncytial knots and increased perivillous fibrin deposits, and greater levels of TNF-α, TGF-β1and Flt-1 were detected in placentas from early-onset PE. Levels of IL-10, VEGF and PlGF were decreased in PE versus normotensive placentas. Both the TNF-α/IL-10 and sFlt-1/PlGF ratios were higher in placental homogenate of early-onset PE than late-onset PE and control groups. The more severe lesions and the imbalance between TNF-α/IL-10 and PlGF/sFlt-1 in placentas from early-onset PE allows differentiation of early and late-onset PE and suggests higher placental impairment in early-onset PE.
Department of Gynaecology and Obstetrics, Botucatu Medical School, São Paulo State University, 18618-970, Botucatu, São Paulo, Brazil.
Ingrid C
IC
Weel
Department of Pathology and Laboratory Medicine, Weill Cornell Medical College - New York Presbyterian Hospital, New York, United States of America.
Rebecca N
RN
Baergen
Department of Gynaecology and Obstetrics, Botucatu Medical School, São Paulo State University, 18618-970, Botucatu, São Paulo, Brazil.
Mariana
M
Romão-Veiga
Department of Gynaecology and Obstetrics, Botucatu Medical School, São Paulo State University, 18618-970, Botucatu, São Paulo, Brazil.
Vera T
VT
Borges
Department of Microbiology and Immunology, Institute of Biosciences, São Paulo State University, 18618-970, Botucatu, São Paulo, Brazil.
Vanessa R
VR
Ribeiro
Department of Obstetrics and Gynecology, Weill Cornell Medical College, New York, United States of America.
Steven S
SS
Witkin
Department of Gynaecology and Obstetrics, Botucatu Medical School, São Paulo State University, 18618-970, Botucatu, São Paulo, Brazil.
Camila
C
Bannwart-Castro
Department of Gynaecology and Obstetrics, Botucatu Medical School, São Paulo State University, 18618-970, Botucatu, São Paulo, Brazil.
Jose C
JC
Peraçoli
Department of Gynaecology and Obstetrics, Botucatu Medical School, São Paulo State University, 18618-970, Botucatu, São Paulo, Brazil.
Leandro
L
De Oliveira
Department of Microbiology and Immunology, Institute of Biosciences, São Paulo State University, 18618-970, Botucatu, São Paulo, Brazil.
Maria T
MT
Peraçoli
D000328:Adult; D017668:Age of Onset; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism / Q000473:pathology; D000072483:Placenta Growth Factor / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000378:metabolism* / Q000473:pathology; D011247:Pregnancy; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000378:metabolism*
C508609:IL10 protein, human; C000608036:PGF protein, human; D014409:Tumor Necrosis Factor-alpha; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D016753:Interleukin-10; D000072483:Placenta Growth Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; C501162:FLT1 protein, human; D040281:Vascular Endothelial Growth Factor Receptor-1