Placental development during early pregnancy: Effects of embryo origin on expression of chemokine ligand twelve (CXCL12).
The aim was to localize chemokine ligand twelve (CXCL12) in sheep placental tissues during early gestation and after assisted reproductive technologies (ART). Uteri were collected from naturally (NAT) mated ewes and ewes receiving embryo transfer (ET), in vitro fertilization (IVF) or in vitro activation (IVA). CXCL12 was immunolocalized to endometrial stroma, glands, and trophoblast. Greater CXCL12 immunoreactivity was present in trophoblast on day 22 and 24 and in NAT ewes compared to IVF and IVA. Increased CXCL12 expression suggests CXCL12 promotes implantation and placentation. Decreased CXCL12 in IVF and IVA embryos, may compromise pregnancy establishment when utilizing ART methods.
Department of Animal and Range Sciences, New Mexico State University, 2980 South Espina Street, Las Cruces, NM, 88003, USA.
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Quinn
Center for Nutrition and Pregnancy and Department of Animal Sciences, North Dakota State University, Fargo, ND, 58108-6050, USA.
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Reynolds
Center for Nutrition and Pregnancy and Department of Animal Sciences, North Dakota State University, Fargo, ND, 58108-6050, USA.
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Grazul-Bilska
Center for Nutrition and Pregnancy and Department of Animal Sciences, North Dakota State University, Fargo, ND, 58108-6050, USA; Advanced Imaging and Microscopy Core Laboratory, Dept. 7630, North Dakota State University, Fargo, ND, 58108-6050, USA.
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Borowicz
Department of Animal and Range Sciences, New Mexico State University, 2980 South Espina Street, Las Cruces, NM, 88003, USA. Electronic address: ryashley@nmsu.edu.
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Ashley
D000818:Animals; D054377:Chemokine CXCL12 / Q000378:metabolism*; D010064:Embryo Implantation / Q000502:physiology; D004624:Embryo Transfer / Q000662:veterinary*; D005260:Female; D010920:Placenta / Q000378:metabolism*; D010929:Placentation / Q000502:physiology*; D011247:Pregnancy; D027724:Reproductive Techniques, Assisted / Q000662:veterinary*; D012756:Sheep
D054377:Chemokine CXCL12
Assisted reproductive technologies; CXCL12; Implantation; Placentation