Anthracene-based fluorescent nanoprobes for singlet oxygen detection in biological media.
We have developed a novel singlet oxygen nanoprobe based on 9,10-anthracenedipropionic acid covalently bound to mesoporous silica nanoparticles. The nanoparticle protects the probe from interactions with proteins, which detract from its ability to detect singlet oxygen. In vitro studies show that the nanoprobe is internalized by cells and is distributed throughout the cytoplasm, thus being capable of detecting intracellularly-generated singlet oxygen.
Grup d'Enginyeria Molecular, Institut Químic de Sarrià, Universitat Ramon Llull, Via Augusta 390, E-08017 Barcelona, Spain.
Roger
R
Bresolí-Obach
Grup d'Enginyeria Molecular, Institut Químic de Sarrià, Universitat Ramon Llull, Via Augusta 390, E-08017 Barcelona, Spain.
Jaume
J
Nos
Departament de Bioquímica i Biologia Molecular, Facultat de Biologia, Universitat de Barcelona, Avinguda Diagonal 645, E-08028 Barcelona, Spain.
Margarita
M
Mora
Departament de Bioquímica i Biologia Molecular, Facultat de Biologia, Universitat de Barcelona, Avinguda Diagonal 645, E-08028 Barcelona, Spain.
Maria Lluïsa
ML
Sagristà
Grup d'Enginyeria Molecular, Institut Químic de Sarrià, Universitat Ramon Llull, Via Augusta 390, E-08017 Barcelona, Spain.
Rubén
R
Ruiz-González
Grup d'Enginyeria Molecular, Institut Químic de Sarrià, Universitat Ramon Llull, Via Augusta 390, E-08017 Barcelona, Spain. Electronic address: santi.nonell@iqs.url.edu.
Santi
S
Nonell
D000873:Anthracenes / Q000737:chemistry*; D003593:Cytoplasm / Q000737:chemistry / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D026082:Singlet Oxygen / Q000737:chemistry / Q000302:isolation & purification*
D000873:Anthracenes; D005456:Fluorescent Dyes; D026082:Singlet Oxygen; D012822:Silicon Dioxide; C034020:anthracene
Anthracene dipropionic acid; Confocal microscopy; Fluorescent probe; HeLa cells; Silica nanoparticles; Singlet oxygen