Involvement of IL-10 and TGF-β in HLA-E-mediated neuroblastoma migration and invasion.
Human leukocyte antigen (HLA)-E is highly expressed in a variety of tumors and, in addition to immune escape, may promote tumor growth via other mechanisms. However, the role of HLA-E in neuroblastoma (NB) migration and invasion is unknown. In the present study, HLA-E expression in human NB tumors was measured by immunohistochemistry. The effect of HLA-E on NB cell migration and invasion was studied in vitro and in vivo, as well as the effect of HLA-E on natural killer (NK)-cell cytotoxicity. HLA-E was expressed in 70.2% of the NB tumor tissues examined. HLA-E expression by NB cells inhibited NK-cell cytotoxicity and induced the release of interleukin (IL)-10 and transforming growth factor (TGF)-β1. HLA-E and the released cytokines enhanced the ability of NB cells migration and invasion. NK cell infusion did not inhibit the growth of NB cells with high HLA-E expression but instead increased the number of metastatic cells in the bone marrow. Taken together, the results indicate that IL-10 and TGF-β are involved in HLA-E-mediated NB migration and invasion. Thus, HLA-E may be a new treatment target in NB.
Department of Pediatric Oncology, Sun Yat-sen University Cancer Center, Guangzhou, 510060, China.|State Key Laboratory of Oncology in South China, Guangzhou, 510060, China.|Collaborative Innovation Center of Cancer Medicine, Guangzhou, 510060, China.
Zijun
Z
Zhen
Department of Pediatric Oncology, Sun Yat-sen University Cancer Center, Guangzhou, 510060, China.|State Key Laboratory of Oncology in South China, Guangzhou, 510060, China.|Collaborative Innovation Center of Cancer Medicine, Guangzhou, 510060, China.
Xiaofang
X
Guo
Department of Pediatric Oncology, Sun Yat-sen University Cancer Center, Guangzhou, 510060, China.|State Key Laboratory of Oncology in South China, Guangzhou, 510060, China.|Collaborative Innovation Center of Cancer Medicine, Guangzhou, 510060, China.
Ru
R
Liao
Department of Pediatric Oncology, Sun Yat-sen University Cancer Center, Guangzhou, 510060, China.|Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China.
Kaibin
K
Yang
Department of Pediatric Oncology, Sun Yat-sen University Cancer Center, Guangzhou, 510060, China.|Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China.
Litong
L
Ye
Department of Pediatric Oncology, Sun Yat-sen University Cancer Center, Guangzhou, 510060, China.|Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China.
Zhiyao
Z
You
D048708:Cell Growth Processes / Q000502:physiology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology; D018572:Disease-Free Survival; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis* / Q000276:immunology; D006801:Humans; D016753:Interleukin-10 / Q000276:immunology / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009447:Neuroblastoma / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D016212:Transforming Growth Factor beta / Q000276:immunology / Q000378:metabolism*; D000097625:HLA-E Antigens
D015395:Histocompatibility Antigens Class I; D016753:Interleukin-10; D016212:Transforming Growth Factor beta; D000097625:HLA-E Antigens; C508609:IL10 protein, human
HLA-E; IL-10; invasion; migration; neuroblastoma