The evaluation of β-(1 → 3)-nonaglucoside as an anti-Candida albicans immune response inducer.
Synthetically prepared bovine serum albumin (BSA) conjugate of linear β-(1 → 3)-nonaglucoside ligand (G9) has been applied as a biological response immunomodulator in vivo and ex vivo. Active immunization of Balb/c mice revealed effective induction of specific humoral responses in comparison with Candida β-D-glucan and Candida whole cells. Induced post-vaccination serum exhibited a growth-inhibition effect on the multi-azole-resistant clinical strain Candida albicans CCY 29-3-164 in experimental mucocutaneous infection ex vivo. Evaluation of immune cell proliferation and the cytotoxic potential of the G9-ligand has revealed its bioavailability and an immunostimulative effect in vaccination-sensitized Balb/c mice splenocytes and RAW 264.7 macrophages.
Department of Immunochemistry of Glycoconjugates, Center for Glycomics, Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia.
Ema
E
Paulovičová
Department of Immunochemistry of Glycoconjugates, Center for Glycomics, Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia.
Lucia
L
Paulovičová
Department of Immunochemistry of Glycoconjugates, Center for Glycomics, Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia.
Ružena
R
Pilišiová
Department of Cellular Pharmacology, Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Dubravska cesta 9, 84236, Bratislava, Slovakia.
Viera
V
Jančinová
Laboratory of Glycoconjugate Chemistry, N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Dmitry V
DV
Yashunsky
Laboratory of Glycoconjugate Chemistry, N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Alexander A
AA
Karelin
Laboratory of Glycoconjugate Chemistry, N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Yury E
YE
Tsvetkov
Laboratory of Glycoconjugate Chemistry, N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Nikolay E
NE
Nifantiev
D000818:Animals; D000908:Antibodies, Fungal / Q000097:blood; D000946:Antigens, Fungal / Q000276:immunology; D002176:Candida albicans / Q000276:immunology*; D002177:Candidiasis / Q000097:blood / Q000382:microbiology / Q000517:prevention & control*; D002452:Cell Count; D049109:Cell Proliferation; D005260:Female; D062610:Fungal Polysaccharides / Q000276:immunology*; D005960:Glucosides / Q000276:immunology; D025301:Hyphae / Q000276:immunology; D056724:Immunity, Humoral; D051379:Mice; D008807:Mice, Inbred BALB C; D000067996:RAW 264.7 Cells; D014611:Vaccination
D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D062610:Fungal Polysaccharides; D005960:Glucosides