Proteomic and Metabolomic Analyses of Leaf from Clematis terniflora DC. Exposed to High-Level Ultraviolet-B Irradiation with Dark Treatment.
Clematis terniflora DC. has potential pharmaceutical value; on the contrary, high-level UV-B irradiation with dark treatment led to the accumulation of secondary metabolites. Metabolomic and proteomic analyses of leaf of C. terniflora were performed to investigate the systematic response mechanisms to high-level UV-B irradiation with dark treatment. Metabolites related to carbohydrates, fatty acids, and amino acids and/or proteins related to stress, cell wall, and amino acid metabolism were gradually increased in response to high-level UV-B irradiation with dark treatment. On the basis of cluster analysis and mapping of proteins related to amino acid metabolism, the abundances of S-adenosylmethionine synthetase and cysteine synthase as well as 1,1-diphenyl-2-picrylhydrazyl scavenging activity were gradually increased in response to high-level UV-B irradiation with dark treatment. Furthermore, the abundance of dihydrolipoyl dehydrogenase/glutamate dehydrogenase and the content of γ-aminobutyric acid were also increased following high-level UV-B irradiation with dark treatment. Taken together, these results suggest that high-level UV-B irradiation with dark treatment induces the activation of reactive oxygen species scavenging system and γ-aminobutyric acid shunt pathway in leaf of C. terniflora.
College of Biomedical Engineering & Instrument Science, Zhejiang University , Zheda Road 38, Hangzhou 310027, China.|National Institute of Crop Science, National Agriculture and Food Research Organization, Kannondai 2-1-18, Tsukuba 305-8518, Japan.
Bingxian
B
Yang
National Institute of Crop Science, National Agriculture and Food Research Organization, Kannondai 2-1-18, Tsukuba 305-8518, Japan.
Xin
X
Wang
College of Biomedical Engineering & Instrument Science, Zhejiang University , Zheda Road 38, Hangzhou 310027, China.
Cuixia
C
Gao
College of Biomedical Engineering & Instrument Science, Zhejiang University , Zheda Road 38, Hangzhou 310027, China.
Meng
M
Chen
College of Biomedical Engineering & Instrument Science, Zhejiang University , Zheda Road 38, Hangzhou 310027, China.
Qijie
Q
Guan
College of Biomedical Engineering & Instrument Science, Zhejiang University , Zheda Road 38, Hangzhou 310027, China.
Jingkui
J
Tian
National Institute of Crop Science, National Agriculture and Food Research Organization, Kannondai 2-1-18, Tsukuba 305-8518, Japan.
Setsuko
S
Komatsu
D039324:Clematis / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects*; D016000:Cluster Analysis; D016166:Free Radical Scavengers / Q000378:metabolism; D055442:Metabolome / Q000528:radiation effects; D055432:Metabolomics / Q000379:methods*; D017440:Photoperiod; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects*; D040901:Proteomics / Q000379:methods*; D017382:Reactive Oxygen Species / Q000378:metabolism; D014466:Ultraviolet Rays; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; D005680:gamma-Aminobutyric Acid
Clematis terniflora DC.; dark treatment; leaf; metabolomics; proteomics; ultraviolet-B irradiation