Focusing super resolution on the cytoskeleton.
Super resolution imaging is becoming an increasingly important tool in the arsenal of methods available to cell biologists. In recognition of its potential, the Nobel Prize for chemistry was awarded to three investigators involved in the development of super resolution imaging methods in 2014. The availability of commercial instruments for super resolution imaging has further spurred the development of new methods and reagents designed to take advantage of super resolution techniques. Super resolution offers the advantages traditionally associated with light microscopy, including the use of gentle fixation and specimen preparation methods, the ability to visualize multiple elements within a single specimen, and the potential to visualize dynamic changes in living specimens over time. However, imaging of living cells over time is difficult and super resolution imaging is computationally demanding. In this review, we discuss the advantages/disadvantages of different super resolution systems for imaging fixed live specimens, with particular regard to cytoskeleton structures.
School of Molecular Biosciences, Washington State University, Pullman, WA, USA.
Eric A
EA
Shelden
School of Molecular Biosciences, Washington State University, Pullman, WA, USA.
Zachary T
ZT
Colburn
School of Molecular Biosciences, Washington State University, Pullman, WA, USA.
Jonathan C R
JC
Jones
cytoskeleton; live cell imaging; microscopy