Three-Dimensional Control of DNA Hybridization by Orthogonal Two-Color Two-Photon Uncaging.
We successfully introduced two-photon-sensitive photolabile groups ([7-(diethylamino)coumarin-4-yl]methyl and p-dialkylaminonitrobiphenyl) into DNA strands and demonstrated their suitability for three-dimensional photorelease. To visualize the uncaging, we used a fluorescence readout based on double-strand displacement in a hydrogel and in neurons. Orthogonal two-photon uncaging of the two cages is possible, thus enabling complex scenarios of three-dimensional control of hybridization with light.
Institute of Organic Chemistry and Chemical Biology, Goethe-University Frankfurt, Max-von-Laue-Strasse 9, 60438, Frankfurt am Main, Germany.
Manuela A H
MA
Fichte
Institute of Organic Chemistry and Chemical Biology, Goethe-University Frankfurt, Max-von-Laue-Strasse 9, 60438, Frankfurt am Main, Germany.
Xenia M M
XM
Weyel
Imaging Facility, Max Planck Institute for Brain Research, Max-von-Laue-Strasse 4, 60438, Frankfurt am Main, Germany.
Stephan
S
Junek
Institute of Organic Chemistry and Chemical Biology, Goethe-University Frankfurt, Max-von-Laue-Strasse 9, 60438, Frankfurt am Main, Germany.
Florian
F
Schäfer
Centre National de la Recherche Scientifique, Université de Strasbourg, Unité Mixte de Recherche 7199, Laboratoire de Conception et Application de Molécules Bioactives, Équipe de Chimie et Neurobiologie Moléculaire, 67400, Illkirch, France.
Cyril
C
Herbivo
Centre National de la Recherche Scientifique, Université de Strasbourg, Unité Mixte de Recherche 7199, Laboratoire de Conception et Application de Molécules Bioactives, Équipe de Chimie et Neurobiologie Moléculaire, 67400, Illkirch, France.
Maurice
M
Goeldner
Centre National de la Recherche Scientifique, Université de Strasbourg, Unité Mixte de Recherche 7199, Laboratoire de Conception et Application de Molécules Bioactives, Équipe de Chimie et Neurobiologie Moléculaire, 67400, Illkirch, France.
Alexandre
A
Specht
Institute of Physical and Theoretical Chemistry, Goethe University Frankfurt, Max-von-Laue-Strasse 7, 60438, Frankfurt am Main, Germany.
Josef
J
Wachtveitl
Institute of Organic Chemistry and Chemical Biology, Goethe-University Frankfurt, Max-von-Laue-Strasse 9, 60438, Frankfurt am Main, Germany. heckel@uni-frankfurt.de.
Alexander
0000-0003-3541-4548
A
Heckel
D003116:Color; D004247:DNA / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D017785:Photons
D004247:DNA
DNA; hydrogels; photochemistry; two-photon uncaging; wavelength selectivity