Development of novel electrically conductive scaffold based on hyperbranched polyester and polythiophene for tissue engineering applications.
A novel electrically conductive scaffold containing hyperbranched aliphatic polyester (HAP), polythiophene (PTh), and poly(ε-caprolactone) (PCL) for regenerative medicine application was succesfully fabricated via electrospinning technique. For this purpose, the HAP (G4; fourth generation) was synthesized via melt polycondensation reaction from tris(methylol)propane and 2,2-bis(methylol)propionic acid (bis-MPA). Afterward, the synthesized HAP was functionalized with 2-thiopheneacetic acid in the presence of N,N-dicyclohexyl carbodiimide, and N-hydroxysuccinimide as coupling agent and catalyst, respectively, to afford a thiophene-functionalized G4 macromonomer. This macromonomer was subsequently used in chemical oxidation copolymerization with thiophene monomer to produce a star-shaped PTh with G4 core (G4-PTh). The solution of the G4-PTh, and PCL was electrospun to produce uniform, conductive, and biocompatible nanofibers. The conductivity, hydrophilicity, and mechanical properties of these nanofibers were investigated. The biocompatibility of the electrospun nanofibers were evaluated by assessing the adhesion and proliferation of mouse osteoblast MC3T3-E1 cell line and in vitro degradability to demonstrate their potential uses as a tissue engineering scaffold. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2673-2684, 2016.
Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, P.O. Box: 51656-65811, Tabriz, Islamic Republic of Iran.
Mehdi
M
Jaymand
Department of Chemistry, Payame Noor University, P.O. BOX: 19395-3697, Tehran, Islamic Republic of Iran.
Raana
R
Sarvari
Department of Chemistry, Payame Noor University, P.O. BOX: 19395-3697, Tehran, Islamic Republic of Iran.
Parisa
P
Abbaszadeh
Department of Chemistry, Payame Noor University, P.O. BOX: 19395-3697, Tehran, Islamic Republic of Iran. b_massoumi@pnu.ac.ir.
Bakhshali
B
Massoumi
Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, P.O. Box: 51656-65811, Tabriz, Islamic Republic of Iran.
Morteza
M
Eskandani
Stem Cell and Tissue Engineering Research Laboratory, Sahand University of Technology, P.O. Box: 51335-1996, Tabriz, Islamic Republic of Iran.|Chemical Engineering Department, Faculty of Engineering, Azarbaijan Shahid Madani University, P.O. Box: 53751-71379, Tabriz, Islamic Republic of Iran.
Younes
Y
Beygi-Khosrowshahi
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002460:Cell Line; D002470:Cell Survival; D004553:Electric Conductivity; D008422:Materials Testing; D051379:Mice; D057139:Nanofibers / Q000737:chemistry / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology*; D011091:Polyesters / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D013876:Thiophenes / Q000737:chemistry*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
D001672:Biocompatible Materials; D011091:Polyesters; D011108:Polymers; D013876:Thiophenes; C066730:polythiophene
biocompatibility; conducting scaffold; electrospinning; polythiophene; tissue engineering