[Relationship between PMI and ATR-FTIR Spectral Changes in Swine Costal Cartilages and Ribs].
OBJECTIVE
To analyze postmortem chemical changes in Landrace costal cartilages and ribs using attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, and to provide a novel technique for estimation of postmortem interval (PMI).


METHODS
The swines were sacrificed by hemorrhage and their costal cartilages and ribs were kept in 20 degrees C. The chemical analysis of the costal cartilages and ribs were performed using ATR-FTIR every 72 h. The correlation between the certain spectral parameters and PMI was also analyzed. The time-dependent changes of costal cartilages were more significant than ribs.


RESULTS
There were no obvious changes for the main absorbance bands position, and some absorbance band ratios showed time-dependent changes and significant correlations with the PMI.


CONCLUSIONS
ATR-FTIR has the ability to analyze postmortem chemical changes of the swine costal cartilages and ribs, and it can be a new method to estimate PMI based on spectroscopy.
Yao
Y
Yao
Qi
Q
Wang
Xiao-li
XL
Jing
Bing
B
Li
Yin-ming
YM
Zhang
Zhi-jun
ZJ
Wang
Cheng-zhi
CZ
Li
Han-cheng
HC
Lin
Ji
J
Zhang
Ping
P
Huang
Zhen-yuan
ZY
Wang
D000818:Animals; D001344:Autopsy; D066186:Costal Cartilage; D049429:Forensic Pathology / Q000379:methods; D006470:Hemorrhage; D023421:Models, Animal; D011180:Postmortem Changes; D051381:Rats; D017207:Rats, Sprague-Dawley; D012044:Regression Analysis; D012272:Ribs; D017550:Spectroscopy, Fourier Transform Infrared; D013552:Swine; D013997:Time Factors