Strongyloides stercoralis diagnostic polypeptides for human strongyloidiasis and their proteomic analysis.
Human strongyloidiasis is a deleterious gastrointestinal disease mainly caused by Strongyloides stercoralis infection. Strongyloides stercoralis is a soil-transmitted helminthiasis that is distributed around the globe. Although definitive diagnosis is carried out through the detection of parasite objects in human stool samples, the development of reliable immunological assays is an important alternative approach for supportive diagnosis. We characterized the two sensitive and specific bands of S. stercoralis filariform larvae that reacted with human strongyloidiasis sera based on immunoblot analysis. Serum samples obtained from strongyloidiasis patients showed a sensitivity of 90 and 80 % at the approximate molecular mass of 26 and 29-kDa polypeptide bands, respectively. The reactive specificity of the 26-kDa band was 76.5 % while for the 29-kDa band was 92.2 %. Proteomic analysis identified the 26-kDa band protein was 14-3-3 protein zeta, while the 29-kDa band protein was ADP/ATP translocase 4. The results provided a basic framework for further studies regarding the potential of the S. stercoralis recombinant antigen to become a leading to diagnostic tool.
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.|Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Rutchanee
R
Rodpai
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.|Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Pewpan M
PM
Intapan
Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.|Faculty of Medicine, Mahasarakham University, Maha Sarakham, Thailand.
Tongjit
T
Thanchomnang
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.|Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.|Faculty of Medicine, Mahasarakham University, Maha Sarakham, Thailand.
Oranuch
O
Sanpool
Department of Medical Technology, School of Allied Health Sciences and Public Health, Walailak University, Nakhon Si Thammarat, Thailand.
Penchom
P
Janwan
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.|Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Porntip
P
Laummaunwai
Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Chaisiri
C
Wongkham
Khon Kaen University Research Instrument Center, Research Affairs, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Tonkla
T
Insawang
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand. wanch_ma@kku.ac.th.|Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand. wanch_ma@kku.ac.th.
Wanchai
W
Maleewong
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D006801:Humans; D010455:Peptides / Q000276:immunology*; D040901:Proteomics; D011994:Recombinant Proteins; D012680:Sensitivity and Specificity; D017171:Strongyloides stercoralis / Q000276:immunology* / Q000302:isolation & purification; D013322:Strongyloidiasis / Q000175:diagnosis* / Q000469:parasitology
D000947:Antigens, Helminth; D010455:Peptides; D011994:Recombinant Proteins
Diagnosis; Immunoblot analysis; Proteomic analysis; Strongyloides stercoralis; Strongyloidiasis