Efficacy of UV-C photolysis of bisphenol A on transcriptome alterations of genes in zebrafish embryos.
The purpose of this study was to investigate the efficacy of UV-C direct photolysis of bisphenol A (BPA) as a remediation method of BPA contamination. We used zebrafish embryos as a model organism to test the toxicity and residual biological activity by measuring cytochrome P4501A1 (CYP1A), aromatase B (Aro B) and heat shock proteins (HSP-70) transcript levels. The mRNA levels of CYP1A gene increased about two fold while exposure of zebrafish embryos at 72 hpf resulted in significant induction (P = 0.048) of Aro B at 100 µg/L of BPA. Exposure of zebrafish embryos at 72 hpf to increasing concentrations of BPA resulted in significant induction (P = 0.0031) of HSP-70 transcript level. UV treatment of BPA resulted in a significant reduction in toxicity by reducing mortality of zebrafish embryos. The results suggest that UV-C direct photolysis may be an effective method for remediation of BPA contamination. Further studies will be necessary for better understanding of the identity and relative activity of the UV degradation by-products.
a Institute of Environmental Science & Engineering (IESE), School of Civil & Environmental Engineering (SCEE), National University of Sciences & Technology (NUST) , Islamabad , Pakistan.
Asma
A
Saeed
a Institute of Environmental Science & Engineering (IESE), School of Civil & Environmental Engineering (SCEE), National University of Sciences & Technology (NUST) , Islamabad , Pakistan.
Imran
I
Hashmi
b Department of Biological Science , University of Calgary , Calgary , Alberta , Canada.
Ava
A
Zare
c Department of Civil Engineering , University of Calgary , Calgary , Alberta , Canada.
Mitra
M
Mehrabani-Zeinabad
c Department of Civil Engineering , University of Calgary , Calgary , Alberta , Canada.
Gopal
G
Achari
b Department of Biological Science , University of Calgary , Calgary , Alberta , Canada.
Hamid R
HR
Habibi
D000818:Animals; D001141:Aromatase / Q000235:genetics / Q000378:metabolism; D001559:Benzhydryl Compounds / Q000737:chemistry* / Q000633:toxicity; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000187:drug effects; D005819:Genetic Markers; D010636:Phenols / Q000737:chemistry* / Q000633:toxicity; D010782:Photolysis; D059467:Transcriptome / Q000187:drug effects*; D014466:Ultraviolet Rays; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics*; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism; D000098826:Bisphenol A Compounds
D001141:Aromatase; D001559:Benzhydryl Compounds; D019363:Cytochrome P-450 CYP1A1; D005819:Genetic Markers; D010636:Phenols; D029961:Zebrafish Proteins; C440728:Cyp19a1b protein, zebrafish; C006780:bisphenol A; D000098826:Bisphenol A Compounds
Bisphenol A; QPCR; UV photolysis; biomarker genes; endocrine disruption; zebrafish embryos