Isolation and characterization of a bacteriophage phiEap-2 infecting multidrug resistant Enterobacter aerogenes.
Enterobacter aerogenes (Enterobacteriaceae) is an important opportunistic pathogen that causes hospital-acquired pneumonia, bacteremia, and urinary tract infections. Recently, multidrug-resistant E. aerogenes have been a public health problem. To develop an effective antimicrobial agent, bacteriophage phiEap-2 was isolated from sewage and its genome was sequenced because of its ability to lyse the multidrug-resistant clinical E. aerogenes strain 3-SP. Morphological observations suggested that the phage belongs to the Siphoviridae family. Comparative genome analysis revealed that phage phiEap-2 is related to the Salmonella phage FSL SP-031 (KC139518). All of the structural gene products (except capsid protein) encoded by phiEap-2 had orthologous gene products in FSL SP-031 and Serratia phage Eta (KC460990). Here, we report the complete genome sequence of phiEap-2 and major findings from the genomic analysis. Knowledge of this phage might be helpful for developing therapeutic strategies against E. aerogenes.
College of Food Science, South China Agricultural University, Guangzhou, 510642, China.|Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 100071, China.
Erna
E
Li
Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 100071, China.
Xiao
X
Wei
College of Food Science, Henan Institute of Science and Technology, Xinxiang, 453003, China.|Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 100071, China.
Yanyan
Y
Ma
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, China.
Zhe
Z
Yin
Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 100071, China.
Huan
H
Li
Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 100071, China.
Weishi
W
Lin
Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 100071, China.
Xuesong
X
Wang
Key Laboratory of Risk Assessment and Control for Environment and Food Safety, Tianjin Institute of Health and Environmental Medicine, Tianjin, 300050, China.
Chao
C
Li
Key Laboratory of Risk Assessment and Control for Environment and Food Safety, Tianjin Institute of Health and Environmental Medicine, Tianjin, 300050, China.
Zhiqiang
Z
Shen
College of Food Science, Henan Institute of Science and Technology, Xinxiang, 453003, China.
Ruixiang
R
Zhao
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, China.
Huiying
H
Yang
College of Food Science, South China Agricultural University, Guangzhou, 510642, China.
Aimin
A
Jiang
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, China.
Wenhui
W
Yang
Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 100071, China.
Jing
J
Yuan
Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing, 100071, China.
Xiangna
X
Zhao
D000900:Anti-Bacterial Agents / Q000737:chemistry; D001435:Bacteriophages / Q000235:genetics* / Q000302:isolation & purification*; D002473:Cell Wall / Q000378:metabolism; D016000:Cluster Analysis; D003428:Cross Infection / Q000382:microbiology / Q000821:virology; D004247:DNA / Q000378:metabolism; D004279:DNA, Viral / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial; D021902:Enterobacter aerogenes / Q000821:virology*; D016679:Genome, Viral; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008854:Microscopy, Electron; D010641:Phenotype; D010802:Phylogeny; D012722:Sewage / Q000821:virology; D017906:Siphoviridae / Q000235:genetics; D013696:Temperature
D000900:Anti-Bacterial Agents; D004279:DNA, Viral; D012722:Sewage; D004247:DNA