Structure and expression analysis of genes encoding ADP-glucose pyrophosphorylase large subunit in wheat and its relatives.
ADP-glucose pyrophosphorylase (AGP), which consists of two large subunits (AGP-L) and two small subunits (AGP-S), controls the rate-limiting step in the starch biosynthetic pathway. In this study, a full-length open reading frame (ORF) of AGP-L gene (named as Agp2) in wheat and a series of Agp2 gene sequences in wheat relatives were isolated. The coding region of Agp2 contained 15 exons and 14 introns including a full-length ORF of 1566 nucleotides, and the deduced protein contained 522 amino acids (57.8 kDa). Generally, the phylogenetic tree of Agp2 indicated that sequences from A- and D-genome donor species were most similar to each other and sequences from B-genome donor species contained more variation. Starch accumulation and Agp2 expression in wheat grains reached their peak at 21 and 15 days post anthesis (DPA), respectively.
a Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Xiao-Wei
XW
Zhang
a Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Si-Yu
SY
Li
a Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Ling-Ling
LL
Zhang
a Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Qiang
Q
Yang
a Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Qian-Tao
QT
Jiang
a Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Jian
J
Ma
a Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Peng-Fei
PF
Qi
a Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Wei
W
Li
a Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Guo-Yue
GY
Chen
a Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Xiu-Jin
XJ
Lan
a Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Mei
M
Deng
b Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, AB T1J 4B1, Canada.
Zhen-Xiang
ZX
Lu
c CSIRO Agriculture Flagship, 306 Carmody Road, St Lucia, Brisbane, QLD 4067, Australia.
Chunji
C
Liu
a Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Yu-Ming
YM
Wei
d Key Laboratory of Southwestern Crop Germplasm Utilization, Ministry of Agriculture, Sichuan Agricultural University, Ya'an, Sichuan 625014, China.
You-Liang
YL
Zheng
D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D051599:Glucose-1-Phosphate Adenylyltransferase / Q000096:biosynthesis / Q000235:genetics*; D016366:Open Reading Frames; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D012639:Seeds / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013213:Starch / Q000096:biosynthesis; D014908:Triticum / Q000201:enzymology* / Q000235:genetics*
D018076:DNA, Complementary; D018744:DNA, Plant; D010940:Plant Proteins; D013213:Starch; D051599:Glucose-1-Phosphate Adenylyltransferase
AGP-L; accumulation de l’amidon; blé; expression; gene structure; starch accumulation; structure génique; wheat