Physiological characterization and true-to-typeness evaluation of in vitro and ex vitro seedlings of Pinus elliottii: A contribution to breeding programs.
Pinus elliottii var. elliottii is a pine species with enormous economic value particularly for timber and resin industries, and is subject of high pressure for genetic improvement and cloning elite genotypes. We have recently developed a robust micropropagation protocol for this species. Plantlets performance needs to be evaluated to validate this protocol for further mass propagation. Micropropagated plantlets and seed-derived plants with similar age and shoot length were compared regarding photosynthesis, carbohydrates and pigments content, water status, DNA content and cell cycle dynamics. Micropropagated plantlets had an overall physiological performance similar to seed-derived plants. In particular, except for the transpiration rate (E), CO2 assimilation rate (A) and total soluble sugars (TSS) content, no major differences between plantlets and seedlings in terms of relative water content (RWC), chlorophyll a fluorescence and pigments content were found. Genetic fidelity analyses support that the micropropagation protocol neither induce DNA content changes nor alterations in cell cycle dynamics.
KLÓN - Innovative Technologies from Cloning, S.A., Cantanhede, Portugal.
Sandra
S
Nunes
Department of Biology & GreenUP, CITAB UP, Faculty of Sciences, University of Porto, Rua do Campo Alegre, 4169-007, Porto, Portugal. Electronic address: csantos@fc.up.pt.
Conceição
C
Santos
Department of Biology and Environment, Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes e Alto Douro, 5000-801, Vila Real, Portugal.
José
J
Moutinho-Pereira
Centre for Environmental and Marine Studies (CESAM), University of Aveiro, 3810-193, Aveiro, Portugal.
Carlos
C
Correia
Centre for Environmental and Marine Studies (CESAM), University of Aveiro, 3810-193, Aveiro, Portugal; Department of Chemistry and CICECO, University of Aveiro, 3810-193, Aveiro, Portugal.
Helena
H
Oliveira
Department of Biology & GreenUP, CITAB UP, Faculty of Sciences, University of Porto, Rua do Campo Alegre, 4169-007, Porto, Portugal; Centre for Environmental and Marine Studies (CESAM), University of Aveiro, 3810-193, Aveiro, Portugal.
José Miguel
JM
Ferreira de Oliveira
KLÓN - Innovative Technologies from Cloning, S.A., Cantanhede, Portugal.
Vanessa Tolentino
VT
Pereira
KLÓN - Innovative Technologies from Cloning, S.A., Cantanhede, Portugal.
Tânia
T
Almeida
KLÓN - Innovative Technologies from Cloning, S.A., Cantanhede, Portugal.
Liliana
L
Marum
Centre for Functional Ecology (CEF), Department of Life Science, University of Coimbra, Calçada Martim de Freitas, 3000-456, Coimbra, Portugal; Department of Chemistry and QOPNA, University of Aveiro, 3810-193, Aveiro, Portugal.
Maria Celeste
MC
Dias
D001947:Breeding / Q000379:methods*; D002241:Carbohydrates / Q000032:analysis; D002453:Cell Cycle; D002734:Chlorophyll / Q000378:metabolism; D000077194:Chlorophyll A; D005453:Fluorescence; D005740:Gases / Q000378:metabolism; D028223:Pinus / Q000502:physiology*; D011003:Ploidies; D036226:Seedlings / Q000502:physiology*; D014867:Water / Q000378:metabolism
D002241:Carbohydrates; D005740:Gases; D014867:Water; D002734:Chlorophyll; D000077194:Chlorophyll A
Genetic stability; Micropropagation; Photosynthesis; Slash pine