Postnatal development of the electrophysiological properties of somatostatin interneurons in the anterior cingulate cortex of mice.
Somatostatin (SST)-positive interneurons in the anterior cingulate cortex (ACC) play important roles in neuronal diseases, memory and cognitive functions. However, their development in the ACC remains unclear. Using postnatal day 3 (P3) to P45 GIN mice, we found that most of the intrinsic membrane properties of SST interneurons in the ACC were developmentally mature after the second postnatal week and that the development of these neurons differed from that of parvalbumin (PV) interneurons in the prefrontal cortex. In addition, electrical coupling between SST interneurons appeared primarily between P12-14. The coupling probability plateaued at approximately P21-30, with a non-age-dependent development of coupling strength. The development of excitatory chemical afferents to SST interneurons occurred earlier than the development of inhibitory chemical afferents. Furthermore, eye closure attenuated the development of electrical coupling probability at P21-30 but had no effect on coupling strength. Eye closure also delayed the development of inhibitory chemical afferent frequency but had no effect on the excitatory chemical afferent amplitude, frequency or rise time. Our data suggest that SST interneurons in the ACC exhibit inherent developmental characteristics distinct from other interneuron subtypes, such as PV interneurons, and that some of these characteristics are subject to environmental regulation.
Department of Neurology, Brain Center, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310058 China.
Geng
G
Pan
Department of Neurobiology, Institute of Neuroscience, Key Laboratory of Medical Neurobiology of the Ministry of Health, Zhejiang Province Key Laboratory of Neurobiology, Collaborative Innovation Center for Brain Science, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310058 China.
Jian-Ming
JM
Yang
Department of Neurology, Brain Center, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310058 China.
Xing-Yue
XY
Hu
Department of Neurology, Brain Center, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310058 China.|Department of Neurobiology, Institute of Neuroscience, Key Laboratory of Medical Neurobiology of the Ministry of Health, Zhejiang Province Key Laboratory of Neurobiology, Collaborative Innovation Center for Brain Science, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310058 China.|Joint Institute for Genetics and Genome Medicine between Zhejiang University and University of Toronto, Zhejiang University, Hangzhou, Zhejiang, 310058 China.
Xiao-Ming
XM
Li
D000200:Action Potentials / Q000502:physiology; D000818:Animals; D055724:Electrophysiological Phenomena / Q000502:physiology*; D005123:Eye / Q000294:innervation; D005260:Female; D049452:Green Fluorescent Proteins / Q000235:genetics; D006179:Gyrus Cinguli / Q000196:embryology*; D007395:Interneurons / Q000166:cytology*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D013004:Somatostatin / Q000378:metabolism*
D049452:Green Fluorescent Proteins; D013004:Somatostatin