Comparative proteomic study of Arabidopsis mutants mpk4 and mpk6.
Arabidopsis MPK4 and MPK6 are implicated in different signalling pathways responding to diverse external stimuli. This was recently correlated with transcriptomic profiles of Arabidopsis mpk4 and mpk6 mutants, and thus it should be reflected also on the level of constitutive proteomes. Therefore, we performed a shot gun comparative proteomic analysis of Arabidopsis mpk4 and mpk6 mutant roots. We have used bioinformatic tools and propose several new proteins as putative MPK4 and MPK6 phosphorylation targets. Among these proteins in the mpk6 mutant were important modulators of development such as CDC48A and phospholipase D alpha 1. In the case of the mpk4 mutant transcriptional reprogramming might be mediated by phosphorylation and change in the abundance of mRNA decapping complex VCS. Further comparison of mpk4 and mpk6 root differential proteomes showed differences in the composition and regulation of defense related proteins. The mpk4 mutant showed altered abundances of antioxidant proteins. The examination of catalase activity in response to oxidative stress revealed that this enzyme might be preferentially regulated by MPK4. Finally, we proposed developmentally important proteins as either directly or indirectly regulated by MPK4 and MPK6. These proteins contribute to known phenotypic defects in the mpk4 and mpk6 mutants.
Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, 783 71 Olomouc, Czech Republic.
Tomáš
T
Takáč
Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, 783 71 Olomouc, Czech Republic.
Pavol
P
Vadovič
Institute for Genomics, Biocomputing &Biotechnology, Mississippi Agricultural and Forestry Experiment Station, Mississippi State University, MS 39759, USA.
Tibor
T
Pechan
Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, 783 71 Olomouc, Czech Republic.
Ivan
I
Luptovčiak
Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, 783 71 Olomouc, Czech Republic.
Olga
O
Šamajová
Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, 783 71 Olomouc, Czech Republic.
Jozef
J
Šamaj
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D001665:Binding Sites; D002374:Catalase / Q000378:metabolism; D055786:Gene Knockout Techniques; D063990:Gene Ontology; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics*; D020125:Mutation, Missense; D009195:Peroxidase / Q000378:metabolism; D010766:Phosphorylation; D018517:Plant Roots / Q000201:enzymology / Q000235:genetics; D011485:Protein Binding; D011499:Protein Processing, Post-Translational; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D000075370:Receptors for Activated C Kinase / Q000378:metabolism; D013312:Stress, Physiological
D029681:Arabidopsis Proteins; D020543:Proteome; C515163:RACK1 protein, Arabidopsis; C457768:REM1 protein, Arabidopsis; D000075370:Receptors for Activated C Kinase; D002374:Catalase; D009195:Peroxidase; C436292:AtMPK4 protein, Arabidopsis; C436293:MPK6 protein, Arabidopsis; D020928:Mitogen-Activated Protein Kinases