Dual-band optical coherence tomography using a single supercontinuum laser source.
We developed a simultaneous visible-light (Vis) and near-infrared (NIR) dual-band optical coherence tomography (OCT) system using a single supercontinuum laser source. The goal was to benchmark our newly developed Vis-OCT against the well-developed NIR-OCT. The Vis-OCT subsystem operated at 91 nm full-width-at-half-maximum (FWHM) bandwidth centered at 566 nm; the NIR-OCT subsystem operated at 93 nm FWHM bandwidth centered at 841 nm. The axial resolutions were 1.8 and 4.4  μm in air for the Vis- and NIR-OCT subsystems, respectively. We compared the respective performances, including anatomical imaging, angiography, absolute retinal blood flow measurements, and spectroscopic analysis for retinal blood oxygen saturation (sO2), between the two subsystems in rodents in vivo. While demonstrating minor discrepancies related to operation wavelengths, both subsystems showed comparable performances in the first three tests. However, we were only able to retrieve sO2 using the Vis-OCT subsystem.
Northwestern University, Department of Biomedical Engineering, 2145 Sheridan Road, Evanston, Illinois 60208, United States.
Siyu
S
Chen
Northwestern University, Department of Biomedical Engineering, 2145 Sheridan Road, Evanston, Illinois 60208, United States.
Xiao
X
Shu
Northwestern University, Department of Biomedical Engineering, 2145 Sheridan Road, Evanston, Illinois 60208, United StatesbBoston University, Department of Medicine, 650 Albany Street, Boston, Massachusetts 02118, United States.
Ji
J
Yi
Northwestern University, Department of Ophthalmology, 300 East Superior Street, Chicago, Illinois 60611, United States.
Amani
A
Fawzi
Northwestern University, Department of Biomedical Engineering, 2145 Sheridan Road, Evanston, Illinois 60208, United StatescNorthwestern University, Department of Ophthalmology, 300 East Superior Street, Chicago, Illinois 60611, United States.
Hao F
HF
Zhang
D000818:Animals; D007259:Infrared Rays; D007834:Lasers; D008027:Light; D051379:Mice; D012039:Regional Blood Flow; D012160:Retina / Q000000981:diagnostic imaging; D013057:Spectrum Analysis; D041623:Tomography, Optical Coherence / Q000295:instrumentation*