Phytochemical and antioxidant activity studies on Ononis angustissima L. aerial parts: isolation of two new flavonoids.
Ononis angustissima aerial parts extract and exudate were subjected to phytochemical and biological studies. Two new natural flavonoids, (3S)-7-hydroxy-4'-methoxy-isoflavanone 3'-β-d-glucopyranoside (1) and kaempferol 3-O-β-d-glucopyranoside-7-O-(2'''-acetyl)-β-d-galactopyranoside (4), and sixteen known compounds were isolated through a bio-oriented approach. Their structural characterisation was achieved using spectroscopic analyses including 2D NMR. The phytochemical profile of the extracts was also performed, and the antioxidant activity of all compounds was tested by three different assays. To get a trend in the results and to compare the antioxidant capacity among the different methods used, the obtained data were transformed to a relative antioxidant capacity index.
a Laboratoire de Valorisation des Ressources Naturelle, Molécules Bioactives et Analyses Physicochimiques et Biologiques (VARENBIOMOL) , Université Constantine 1 , Constantine , Algeria.
Abderrahmane
A
Mezrag
b Dipartimento di Farmacia , Università di Salerno , Fisciano , Italy.
Nicola
N
Malafronte
c Dipartimento di Scienze , Università degli Studi della Basilicata , Potenza , Italy.
Mohamed
M
Bouheroum
b Dipartimento di Farmacia , Università di Salerno , Fisciano , Italy.
Carmen
C
Travaglino
c Dipartimento di Scienze , Università degli Studi della Basilicata , Potenza , Italy.
Daniela
D
Russo
c Dipartimento di Scienze , Università degli Studi della Basilicata , Potenza , Italy.
Luigi
L
Milella
d Dipartimento di Medicina Veterinaria e Produzione Animale, Sezione di Tossicologia Alimentare e Veterinaria , Università di Napoli Federico II , Napoli , Italy.
Lorella
L
Severino
b Dipartimento di Farmacia , Università di Salerno , Fisciano , Italy.
Nunziatina
N
De Tommasi
e Dipartimento di Farmacia , Università di Pisa , Pisa , Italy.
Alessandra
A
Braca
b Dipartimento di Farmacia , Università di Salerno , Fisciano , Italy.
Fabrizio
F
Dal Piaz
D000975:Antioxidants / Q000494:pharmacology*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification*; D000068757:Ononis / Q000737:chemistry*; D064209:Phytochemicals / Q000032:analysis; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*
D000975:Antioxidants; D005419:Flavonoids; D064209:Phytochemicals; D010936:Plant Extracts
Fabaceae; Ononis angustissima; antioxidant activity; isoflavonoids; mass spectrometry; phenolic derivatives