Impact of metritis on the generation of reactive oxygen species by circulating phagocytes and plasma lipopolysaccharide concentration in peripartum dairy cows.
This study examined the relationship between postpartum metritis, reactive oxygen species (ROS) generation, and plasma lipopolysaccharide (LPS) concentration in peripartum dairy cows. Blood was collected twice weekly from 2 weeks prepartum through 6 weeks postpartum. Whole blood chemiluminescence (WBCL) was measured using the luminol-enhanced zymosan-stimulated chemiluminescence assay. Cows were examined for uterine health disorders and classified into two groups, healthy (n = 11) and metritis (n = 5). Metritis had a significant effect on WBCL, with cows with metritis having a higher WBCL. Plasma LPS concentrations in cows with metritis were significantly higher than in healthy cows. To examine the effect of LPS on WBCL, blood was sampled in healthy peripartum cows (1 to 2 weeks prepartum, n = 8; 0 to 3 weeks postpartum, n = 11; and 4 to 8 weeks postpartum, n = 8) and incubated with LPS. At 1 endotoxin units/mL of LPS, similar to the plasma LPS concentration in cows with metritis, the WBCL increased in cows at 0 to 3 weeks postpartum. Results indicate that the increase in ROS generation and plasma LPS concentration are associated with metritis, and LPS may be responsible for enhanced ROS generation in early postpartum dairy cows.
Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.
Fumie
F
Magata
Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.
Ikumi
I
Morino
Nippon Beet Sugar Manufacturing Co., Ltd., Obihiro, Japan.
Makoto
M
Teramura
Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.
Ei
E
Tsunoda
Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.
Chiho
C
Kawashima
Department of Applied Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.
Shingo
S
Haneda
Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.
Akio
A
Miyamoto
Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.
Katsuya
K
Kida
Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.
Takashi
T
Shimizu
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood*; D004716:Endometritis / Q000097:blood* / Q000662:veterinary*; D005260:Female; D008070:Lipopolysaccharides / Q000097:blood*; D058725:Peripartum Period / Q000097:blood* / Q000378:metabolism*; D010586:Phagocytes; D011644:Puerperal Disorders / Q000097:blood* / Q000662:veterinary*; D017382:Reactive Oxygen Species / Q000097:blood*
D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species
dairy cow; lipopolysaccharide; metritis; reactive oxygen species