The Novel Gene VpPR4-1 from Vitis pseudoreticulata Increases Powdery Mildew Resistance in Transgenic Vitis vinifera L.
Pathogenesis-related proteins (PRs) can lead to increased resistance of the whole plant to pathogen attack. Here, we isolate and characterize a PR-4 protein (VpPR4-1) from a wild Chinese grape Vitis pseudoreticulata which shows greatly elevated transcription following powdery mildew infection. Its expression profiles under a number of abiotic stresses were also investigated. Powdery mildew, salicylic acid, and jasmonic acid methyl ester significantly increased the VpPR4-1 induction while NaCl and heat treatments just slightly induced VpPR4-1 expression. Abscisic acid and cold treatment slightly affected the expression level of VpPR4-1. The VpPR4-1 gene was overexpressed in 30 regenerated V. vinifera cv. Red Globe via Agrobacterium tumefaciens-mediated transformation and verified by the Western blot. The 26 transgenic grapevines exhibited higher expression levels of PR-4 protein content than wild-type vines and six of them were inoculated with powdery mildew which showed that the growth of powdery mildew was repressed. The powdery mildew-resistance of Red Globe transformed with VpPR4-1 was enhanced inoculated with powdery mildew. Moreover, other powdery mildew resistant genes were associated with feedback regulation since VpPR4-1 is in abundance. This study demonstrates that PR-4 protein in grapes plays a vital role in defense against powdery mildew invasion.
College of Horticulture, Northwest A&F UniversityYangling, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of AgricultureYangling, China; State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F UniversityYangling, China.
Lingmin
L
Dai
College of Horticulture, Northwest A&F UniversityYangling, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of AgricultureYangling, China; State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F UniversityYangling, China.
Dan
D
Wang
College of Horticulture, Northwest A&F UniversityYangling, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of AgricultureYangling, China; State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F UniversityYangling, China.
Xiaoqing
X
Xie
College of Horticulture, Northwest A&F UniversityYangling, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of AgricultureYangling, China; State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F UniversityYangling, China.
Chaohong
C
Zhang
College of Horticulture, Northwest A&F UniversityYangling, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of AgricultureYangling, China; State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F UniversityYangling, China.
Xiping
X
Wang
College of Horticulture, Northwest A&F UniversityYangling, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of AgricultureYangling, China; State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F UniversityYangling, China.
Yan
Y
Xu
College of Horticulture, Northwest A&F UniversityYangling, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of AgricultureYangling, China; State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F UniversityYangling, China.
Yuejin
Y
Wang
College of Horticulture, Northwest A&F UniversityYangling, China; Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of AgricultureYangling, China; State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F UniversityYangling, China.
Jianxia
J
Zhang
grapevine; pathogenesis-related protein-4 (PR-4); powdery mildew; qRT-PCR; transformation