Regulation of retinal angiogenesis by phospholipase C-β3 signaling pathway.
Angiogenesis has an essential role in many pathophysiologies. Here, we show that phospholipase C-β3 (PLC-β3) isoform regulates endothelial cell function and retinal angiogenesis. Silencing of PLC-β3 in human umbilical vein endothelial cells (HUVECs) significantly delayed proliferation, migration and capillary-like tube formation. In addition, mice lacking PLC-β3 showed impaired retinal angiogenesis with delayed endothelial proliferation, reduced endothelial cell activation, abnormal vessel formation and hemorrhage. Finally, tumor formation was significantly reduced in mice lacking PLC-β3 and showed irregular size and shape of blood vessels. These results suggest that regulation of endothelial function by PLC-β3 may contribute to angiogenesis.
Department of Pharmacology, Gene and Cell Therapy Center for Vessel-Associated Disease, Medical Research Institute, Pusan National University School of Medicine, Yangsan, Republic of Korea.
Jung Min
JM
Ha
Department of Anesthesia and Pain Medicine, Pusan National University Hospital, Yangsan, Republic of Korea.
Seung Hoon
SH
Baek
Department of Pharmacology, Gene and Cell Therapy Center for Vessel-Associated Disease, Medical Research Institute, Pusan National University School of Medicine, Yangsan, Republic of Korea.
Young Hwan
YH
Kim
Department of Pharmacology, Gene and Cell Therapy Center for Vessel-Associated Disease, Medical Research Institute, Pusan National University School of Medicine, Yangsan, Republic of Korea.
Seo Yeon
SY
Jin
Department of Pharmacology, Gene and Cell Therapy Center for Vessel-Associated Disease, Medical Research Institute, Pusan National University School of Medicine, Yangsan, Republic of Korea.
Hye Sun
HS
Lee
Department of Physics, Dong-A University, Busan, Republic of Korea.
Sun Ja
SJ
Kim
Department of Anatomy, Pusan National University of Korean Medicine, Yangsan, Republic of Korea.
Hwa Kyoung
HK
Shin
Department of Obstetrics and Gynecology, Pusan National University Hospital, Yangsan, Republic of Korea.
Dong Hyung
DH
Lee
Department of Internal Medicine, Pusan National University Hospital, Busan, Republic of Korea.
Sang Heon
SH
Song
Department of Pharmacology, Gene and Cell Therapy Center for Vessel-Associated Disease, Medical Research Institute, Pusan National University School of Medicine, Yangsan, Republic of Korea.
Chi Dae
CD
Kim
Department of Pharmacology, Gene and Cell Therapy Center for Vessel-Associated Disease, Medical Research Institute, Pusan National University School of Medicine, Yangsan, Republic of Korea.
Sun Sik
SS
Bae
D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D020868:Gene Silencing; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D051379:Mice; D018919:Neovascularization, Physiologic; D054799:Phospholipase C beta / Q000235:genetics / Q000378:metabolism*; D012171:Retinal Vessels / Q000502:physiology*; D015398:Signal Transduction
C518039:PLCB3 protein, human; D054799:Phospholipase C beta