Absence of Bovine leukemia virus (BLV) infection in buffaloes from Amazon and southeast region in Brazil.
Enzootic bovine leucosis is an infectious disease caused by Bovine leukemia virus (BLV) and is well described in bovines. The majority of infected animals are asymptomatic, one to five percent develop lymphoma and from 30 to 50% present a persistent lymphocytosis. The virus occurs naturally in cattle and experimentally in buffaloes, capybaras and rabbits. The occurrence of lymphoma in buffaloes has been attributed to BLV infection by some authors in India and Venezuela, but not confirmed by other studies and little information on natural BLV infection in buffaloes is available. The aim of this study was to evaluate the occurrence of BLV in a sub-sample of buffalo from Amazon and southeast regions in Brazil. Three hundred and fifteen serum samples were negative using commercial AGID and ELISA (ELISA-gp51) which detect anti-BLV glycoprotein gp51 antibodies. The same samples were also evaluated for antibodies to whole virus through a commercial ELISA (ELISA-BLV) in which 77 (24.44%) were found seropositive and two (0.63%) inconclusive. On the other hand, all animals were negative by PCR to BLV targeted to the env and tax genes. These results suggest that ELISA-BLV produces false positive results in buffalo serum (p<0.001). In addition, one buffalo lymphoma sample was negative in both PCR assays used in this study. BLV was not detected in buffaloes from the Amazon basin and the southeast region of Brazil. Serological tests, like ELISA-BLV, usually used for cattle may produce false-positive results for BLV in buffaloes and direct detection tests such as PCR should be chosen in these surveys. The occurrence of lymphoma in buffalo was not associated with BLV infection in the one case analyzed in this work and the etiology and pathogenesis of this disease should be clarified.
Laboratório de Retroviroses, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil; Setor de Medicina Veterinária Preventiva, Escola de Veterinária e Zootecnia, Universidade Federal de Goiás, Goiânia, Goiás, Brazil. Electronic address: cairo@ufg.br.
Cairo H S
CH
De Oliveira
Laboratório de Retroviroses, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. Electronic address: claudiafideles91@hotmail.com.
Cláudia F
CF
Resende
Hospital Veterinário, Instituto de Medicina Veterinária, Universidade Federal do Pará, Castanhal, Pará, Brazil. Electronic address: cmagno@ufpa.br.
Carlos M C
CM
Oliveira
Hospital Veterinário, Instituto de Medicina Veterinária, Universidade Federal do Pará, Castanhal, Pará, Brazil. Electronic address: diomedes@ufpa.br.
José D
JD
Barbosa
Ministério da Agricultura Pecuária e Abastecimento-LANAGRO, Pedro Leopoldo, Minas Gerais, Brazil. Electronic address: antonio.a.fonsecajr@gmail.com.
Antônio A
AA
Fonseca
Laboratório de Retroviroses, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil; Hospital Veterinário, Instituto de Medicina Veterinária, Universidade Federal do Pará, Castanhal, Pará, Brazil. Electronic address: romulocleite@ufmg.br.
Rômulo C
RC
Leite
Laboratório de Retroviroses, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. Electronic address: jenner@ufmg.br.
Jenner K P
JK
Reis
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001938:Brazil; D002020:Buffaloes; D002417:Cattle; D004279:DNA, Viral / Q000097:blood; D016583:Enzootic Bovine Leukosis / Q000097:blood / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005188:False Negative Reactions; D015752:Genes, env; D016355:Genes, pX; D005779:Immunodiffusion / Q000379:methods; D001909:Leukemia Virus, Bovine / Q000276:immunology* / Q000302:isolation & purification*; D008223:Lymphoma / Q000209:etiology / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary
D000914:Antibodies, Viral; D004279:DNA, Viral
AGID; BLV; Buffalo; ELISA; Leucosis; PCR