Recent advances in exploiting ionic liquids for biomolecules: Solubility, stability and applications.
The technological utility of biomolecules (e.g. proteins, enzymes and DNA) can be significantly enhanced by combining them with ionic liquids (ILs) - potentially attractive "green" and "designer" solvents - rather than using in conventional organic solvents or water. In recent years, ILs have been used as solvents, cosolvents, and reagents for biocatalysis, biotransformation, protein preservation and stabilization, DNA solubilization and stabilization, and other biomolecule-based applications. Using ILs can dramatically enhance the structural and chemical stability of proteins, DNA, and enzymes. This article reviews the recent technological developments of ILs in protein-, enzyme-, and DNA-based applications. We discuss the different routes to increase biomolecule stability and activity in ILs, and the design of biomolecule-friendly ILs that can dissolve biomolecules with minimum alteration to their structure. This information will be helpful to design IL-based processes in biotechnology and the biological sciences that can serve as novel and selective processes for enzymatic reactions, protein and DNA stability, and other biomolecule-based applications.
Centre of Research in Ionic Liquids (CORIL), Chemical Engineering Department, Universiti Teknologi PETRONAS, Bandar Seri Iskandar, Malaysia.
Magaret
M
Sivapragasam
Centre of Research in Ionic Liquids (CORIL), Chemical Engineering Department, Universiti Teknologi PETRONAS, Bandar Seri Iskandar, Malaysia.
Muhammad
M
Moniruzzaman
Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka, Japan. m-goto@mail.cstm.kyushu-u.ac.jp.|Center for Future Chemistry, Kyushu University, Fukuoka, Japan. m-goto@mail.cstm.kyushu-u.ac.jp.
Masahiro
M
Goto
D055162:Biocatalysis; D001711:Biotransformation; D004247:DNA / Q000737:chemistry*; D055772:Green Chemistry Technology; D052578:Ionic Liquids / Q000737:chemistry*; D055550:Protein Stability; D011506:Proteins / Q000737:chemistry*; D012995:Solubility
D052578:Ionic Liquids; D011506:Proteins; D004247:DNA
DNA solubility; Enzyme activity; Ionic liquid; Microorganism; Protein stability