Enhancing cellulase production by overexpression of xylanase regulator protein gene, xlnR, in Talaromyces cellulolyticus cellulase hyperproducing mutant strain.
We obtained strains with the xylanase regulator gene, xlnR, overexpressed (HXlnR) and disrupted (DXlnR) derived from Talaromyces cellulolyticus strain C-1, which is a cellulase hyperproducing mutant. Filter paper degrading enzyme activity and cellobiohydrolase I gene expression was the highest in HXlnR, followed by C-1 and DXlnR. These results indicate that the enhancement of cellulase productivity was succeeded by xlnR overexpression.
a Sugar & Bio Technology Department , Tsukishima Kikai Co Ltd , Chuo-ku , Tokyo , Japan.
Naoyuki
N
Okuda
b Research Institute for Sustainable Chemistry , National Institute of Advanced Industrial Science and Technology (AIST) , Higashi-Hiroshima , Hiroshima , Japan.
Tatsuya
T
Fujii
b Research Institute for Sustainable Chemistry , National Institute of Advanced Industrial Science and Technology (AIST) , Higashi-Hiroshima , Hiroshima , Japan.
Hiroyuki
H
Inoue
c Biomedical Research Institute , National Institute of Advanced Industrial Science and Technology (AIST) , Ikeda , Osaka , Japan.
Kazuhiko
K
Ishikawa
b Research Institute for Sustainable Chemistry , National Institute of Advanced Industrial Science and Technology (AIST) , Higashi-Hiroshima , Hiroshima , Japan.
Tamotsu
T
Hoshino
D002480:Cellulase / Q000096:biosynthesis*; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics*; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D059765:Homologous Recombination; D009154:Mutation; D032901:Talaromyces / Q000235:genetics* / Q000378:metabolism*
D005656:Fungal Proteins; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases
Talaromyces cellulolyticus; biomass; cellulase; xlnR