Simultaneous estimation of vitamin K1 and heparin with low limit of detection using cascaded channels fiber optic surface plasmon resonance.
We report an approach for the simultaneous estimation of vitamin K1 (VK1) and heparin via cascaded channel multianalyte sensing probe employing fiber optic surface plasmon resonance technique. Cladding from two well separated portions of the fiber is removed and are respectively coated with thin films of silver (channel-1) and copper (channel-2). The nanohybrid of multiwalled carbon nanotube in chitosan is fabricated over silver layer for the sensing of VK1 whereas core shell nanostructure of polybrene@ZnO is coated over copper layer for the sensing of heparin. Spectral interrogation method is used for the characterization of the sensor. Analyte selectivity of both the channels is performed by carrying out experiments using independent solutions of VK1 and heparin. Experiments performed on the solution of the mixture of VK1 and heparin show red shifts in both the channels on changing the concentration of both the analytes in the mixture. The operating range of both VK1 and heparin is from 0 to 10(-3)g/l. The limit of detection of the sensor is 2.66×10(-4)µg/l and 2.88×10(-4)µg/l for VK1 and heparin respectively which are lower than the reported ones. The additional advantages of the present sensor are low cost, possibility of online monitoring and remote sensing.
Physics Department Indian Institute of Technology Delhi, New Delhi 110016, India.
Rana
R
Tabassum
Physics Department Indian Institute of Technology Delhi, New Delhi 110016, India. Electronic address: bdgupta@physics.iitd.ernet.in.
Banshi D
BD
Gupta
D001774:Blood Chemical Analysis / Q000295:instrumentation; D001780:Blood Coagulation Tests / Q000295:instrumentation*; D045424:Complex Mixtures / Q000097:blood; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation*; D017022:Flow Injection Analysis / Q000295:instrumentation; D006493:Heparin / Q000097:blood*; D006801:Humans; D008832:Microchemistry / Q000295:instrumentation; D037742:Nanotubes, Carbon / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D010837:Vitamin K 1 / Q000097:blood*; D015034:Zinc Oxide / Q000737:chemistry
D045424:Complex Mixtures; D037742:Nanotubes, Carbon; D010837:Vitamin K 1; D006493:Heparin; D015034:Zinc Oxide
Core shell; Heparin; Multiwalled carbon nanotube; Optical fiber; Polybrene; Sensor; Surface plasmon resonance; Vitamin K(1); ZnO