Microarray analysis of microRNA deregulation and angiogenesis-related proteins in endometriosis.
We examined the aberrant microRNA (miRNA) expression profile responsible for the changes in angiogenesis observed in endometriotic lesions. This study revealed characteristic miRNA expression profiles associated with endometriosis in endometrial tissue and endometriotic lesions from the same patient, and their correlation with the most important angiogenic and fibrinolytic factors. miRNA expression was quantified using a microRNA array and reverse-transcription microRNA polymerase chain reaction. Levels of vascular endothelial growth factor A (VEGFA), epidermal growth factor receptor 2 (EGFR2), phosphatase and tensin homolog (PTEN), and C-X-C chemokine receptor type 4 (CXCR4) were quantified using enzyme-linked immunosorbent assay. The endometrial tissue showed significantly lower levels of miR-200b, miR-15a-5p, miR-19b-1-5p, miR-146a-5p, and miR-200c, and higher levels of miR-16-5p, miR-106b-5p, and miR-145-5p. VEGFA was significantly upregulated, whereas EGFR2, PTEN, and CXCR4 were markedly downregulated, in the endometriotic tissues compared to that in the normal endometrial tissues. In conclusion, differences in the miRNA levels could modulate the expression of VEGFA, EGFR2, PTEN, and CXCR4, and may play an important role in the pathogenesis of endometriosis. The higher angiogenic and proteolytic activities observed in the eutopic endometrium might facilitate the implantation of endometrial cells at ectopic sites.
Department of Obstetrics and Gynecology, The Second Hospital of Jilin University, Changchun, Jilin Province, China.
R Q
RQ
Yang
Department of Obstetrics and Gynecology, The Second Hospital of Jilin University, Changchun, Jilin Province, China.
H
H
Teng
Department of Obstetrics and Gynecology, The Second Hospital of Jilin University, Changchun, Jilin Province, China.
X H
XH
Xu
Department of Anesthesia, The Second Hospital of Jilin University, Changchun, Jilin Province, China.
S Y
SY
Liu
Department of Obstetrics and Gynecology, The Second Hospital of Jilin University, Changchun, Jilin Province, China.
Y H
YH
Wang
Department of Obstetrics and Gynecology, The Second Hospital of Jilin University, Changchun, Jilin Province, China.
F J
FJ
Guo
Department of Pathology, The First Hospital of Jilin University, Changchun, Jilin Province, China.
X J
XJ
Liu
D000328:Adult; D004715:Endometriosis / Q000235:genetics* / Q000473:pathology; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis*; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000235:genetics / Q000473:pathology; D051059:PTEN Phosphohydrolase / Q000096:biosynthesis* / Q000235:genetics; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis* / Q000235:genetics; D019718:Receptors, CXCR4 / Q000096:biosynthesis* / Q000235:genetics; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis* / Q000235:genetics
D035683:MicroRNAs; D051059:PTEN Phosphohydrolase; D018719:Erb-b2 Receptor Tyrosine Kinases; D019718:Receptors, CXCR4; D042461:Vascular Endothelial Growth Factor A; C527276:CXCR4 protein, human; C467484:VEGFA protein, human; C508053:ERBB2 protein, human; C494929:PTEN protein, human