Contribution of NADH oxidase to oxidative stress tolerance and virulence of Streptococcus suis serotype 2.
Streptococcus suis is a major swine and zoonotic pathogen that causes severe infections. Previously, we identified 2 Spx regulators in S. suis, and demonstrated that SpxA1 affects oxidative stress tolerance and virulence. However, the mechanism behind SpxA1 function remains unclear. In this study, we targeted 4 genes that were expressed at significantly reduced levels in the spxA1 mutant, to determine their specific roles in adaptation to oxidative stress and virulence potential. The Δnox strain exhibited impaired growth under oxidative stress conditions, suggesting that NADH oxidase is involved in oxidative stress tolerance. Using murine and pig infection models, we demonstrate for the first time that NADH oxidase is required for virulence in S. suis 2. Furthermore, the enzymatic activity of NADH oxidase has a key role in oxidative stress tolerance and a secondary role in virulence. Collectively, our findings reveal that NADH oxidase plays an important part in SpxA1 function and provide a new insight into the pathogenesis of S. suis 2.
a State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University , Wuhan , China.|b Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University , Wuhan , China.|c The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University , Wuhan , China.
Chengkun
C
Zheng
a State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University , Wuhan , China.|b Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University , Wuhan , China.|c The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University , Wuhan , China.
Sujing
S
Ren
a State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University , Wuhan , China.|b Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University , Wuhan , China.|c The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University , Wuhan , China.
Jiali
J
Xu
a State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University , Wuhan , China.|b Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University , Wuhan , China.|c The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University , Wuhan , China.
Xigong
X
Zhao
a State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University , Wuhan , China.|b Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University , Wuhan , China.|c The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University , Wuhan , China.
Guolin
G
Shi
a State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University , Wuhan , China.
Jianping
J
Wu
d College of Food Science and Technology, Huazhong Agricultural University , Wuhan , China.
Jinquan
J
Li
a State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University , Wuhan , China.|b Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University , Wuhan , China.|c The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University , Wuhan , China.
Huanchun
H
Chen
a State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University , Wuhan , China.|b Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University , Wuhan , China.|c The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University , Wuhan , China.
Weicheng
W
Bei
D000818:Animals; D001769:Blood / Q000382:microbiology; D004195:Disease Models, Animal; D015964:Gene Expression Regulation, Bacterial; D051379:Mice; D009097:Multienzyme Complexes / Q000378:metabolism*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D018384:Oxidative Stress; D065288:Serogroup; D013290:Streptococcal Infections / Q000382:microbiology / Q000662:veterinary; D017011:Streptococcus suis / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology; D014774:Virulence; D037521:Virulence Factors / Q000378:metabolism
D009097:Multienzyme Complexes; D037521:Virulence Factors; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases
NADH oxidase; Streptococcus suis; oxidative stress; virulence