The P2X4 purinergic receptor impacts liver regeneration after partial hepatectomy in mice through the regulation of biliary homeostasis.
Many regulatory pathways are involved in liver regeneration after partial hepatectomy (PH), to initiate growth, protect liver cells, and sustain remnant liver functions. Extracellular adenosine triphosphate rises in blood and bile after PH and contributes to liver regeneration, although purinergic receptors and mechanisms remain to be precisely explored. In this work we analyzed during regeneration after PH the involvement of P2X4 purinergic receptors, highly expressed in the liver. P2X4 receptor expression in the liver, liver histology, hepatocyte proliferation, plasma bile acid concentration, bile flow and composition, and lysosome distribution in hepatocytes were studied in wild-type and P2X4 knockout (KO) mice, before and after PH. P2X4 receptors were expressed in hepatocytes and Kupffer cells; in hepatocytes, P2X4 was concentrated in subcanalicular areas closely costained with lysosomal markers. After PH, delayed regeneration, hepatocyte necrosis, and cholestasis were observed in P2X4-KO mice. In P2X4-KO mice, post-PH biliary adaptation was impaired with a smaller increase in bile flow and HCO3 (-) biliary output, as well as altered biliary composition with reduced adenosine triphosphate and lysosomal enzyme release. In line with these data, lysosome distribution and biogenesis were altered in P2X4-KO compared with wild-type mice.



During liver regeneration after PH, P2X4 contributes to the complex control of biliary homeostasis through mechanisms involving pericanalicular lysosomes, with a resulting impact on hepatocyte protection and proliferation. (Hepatology 2016;64:941-953).
INSERM U1174, Université Paris Sud, Orsay, France.|Université Paris Sud, Orsay, France.|UPMC, Université Paris 06, Paris, France.
Aurore
A
Besnard
INSERM U1174, Université Paris Sud, Orsay, France.|Université Paris Sud, Orsay, France.
Julien
J
Gautherot
INSERM U1174, Université Paris Sud, Orsay, France.|Université Paris Sud, Orsay, France.
Boris
B
Julien
INSERM U1174, Université Paris Sud, Orsay, France.|Université Paris Sud, Orsay, France.
Ali
A
Tebbi
INSERM U1174, Université Paris Sud, Orsay, France.|Université Paris Sud, Orsay, France.
Isabelle
I
Garcin
INSERM U1174, Université Paris Sud, Orsay, France.|Université Paris Sud, Orsay, France.
Isabelle
I
Doignon
INSERM U1174, Université Paris Sud, Orsay, France.|Université Paris Sud, Orsay, France.
Noémie
N
Péan
INSERM U1174, Université Paris Sud, Orsay, France.|Université Paris Sud, Orsay, France.|Hépatologie pédiatrique, Hôpital du Kremlin Bicêtre, Le Kremlin Bicêtre, France.
Emmanuel
E
Gonzales
INSERM U1174, Université Paris Sud, Orsay, France.|Université Paris Sud, Orsay, France.
Doris
D
Cassio
INSERM U1174, Université Paris Sud, Orsay, France.|Université Paris Sud, Orsay, France.
Brigitte
B
Grosse
INSERM U1174, Université Paris Sud, Orsay, France.|Université Paris Sud, Orsay, France.
Bingkaï
B
Liu
INSERM U1174, Université Paris Sud, Orsay, France.|Université Paris Sud, Orsay, France.
Hanaa
H
Safya
INSERM U1174, Université Paris Sud, Orsay, France.|Université Paris Sud, Orsay, France.
Florent
F
Cauchois
UPMC, Université Paris 06, Paris, France.|ERL INSERM U 1057, Faculté de Médecine Pierre et Marie Curie, Paris, France.
Lydie
L
Humbert
UPMC, Université Paris 06, Paris, France.|ERL INSERM U 1057, Faculté de Médecine Pierre et Marie Curie, Paris, France.
Dominique
D
Rainteau
INSERM U1174, Université Paris Sud, Orsay, France.|Université Paris Sud, Orsay, France.
Thierry
T
Tordjmann
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001647:Bile Acids and Salts / Q000097:blood; D001659:Biliary Tract / Q000502:physiology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D006498:Hepatectomy; D022781:Hepatocytes / Q000502:physiology; D006706:Homeostasis; D008099:Liver / Q000378:metabolism* / Q000648:ultrastructure; D008115:Liver Regeneration; D008247:Lysosomes / Q000502:physiology*; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D058848:Receptors, Purinergic P2X4 / Q000378:metabolism*
D001647:Bile Acids and Salts; D058848:Receptors, Purinergic P2X4; D000255:Adenosine Triphosphate