Simple and Biocompatible Ion Beam Micropatterning of a Cell-Repellent Polymer on Cell-Adhesive Surfaces to Manipulate Cell Adhesion.
In this paper, the simple and biocompatible micropatterning of cell-repellent poly(N-isopropylacrylamide) (PNIPAAm) on a cell-adhesive substrate by ion beam micropatterning to control cell adhesion is described. Cell-repellent PNIPAAm films spin-coated on cell-adhesive tissue culture polystyrene (TCPS) substrates were selectively irradiated by energetic proton ions at various fluences through a pattern mask, and subsequently developed to create the micropatterns of PNIPAAm. Well-defined negative-type PNIPAAm micropatterns were successfully created on the TCPS substrates at fluences higher than 5 x 10¹⁴ ions/cm², and their chemical properties were dependent on the fluence. Moreover, based on the results of the protein adsorption and in-vitro cell culture tests, 200 µm well-defined micropatterns of mammalian cells were clearly formed on the PNIPAAm-micropatterned TCPS substrates though the preferential adsorption and growth of cells on the TCPS regions due to the strong cell-repellency of PNIPAAm.
In-Tae
IT
Hwang
Chan-Hee
CH
Jung
Chang-Hee
CH
Jung
Jae-Hak
JH
Choi
Kwanwoo
K
Shin
Young-Do
YD
Yoo
D000180:Acrylic Resins / Q000494:pharmacology*; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D007477:Ions; D051379:Mice; D008856:Microscopy, Fluorescence; D055616:Microtechnology / Q000379:methods*; D011108:Polymers / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D014867:Water / Q000737:chemistry
D000180:Acrylic Resins; D001672:Biocompatible Materials; D007477:Ions; D011108:Polymers; D014867:Water; C052970:poly-N-isopropylacrylamide