Differentiation of Induced Pluripotent Stem Cells to Lentoid Bodies Expressing a Lens Cell-Specific Fluorescent Reporter.
Curative approaches for eye cataracts and other eye abnormalities, such as myopia and hyperopia currently suffer from a lack of appropriate models. Here, we present a new approach for in vitro growth of lentoid bodies from induced pluripotent stem (iPS) cells as a tool for ophthalmological research. We generated a transgenic mouse line with lens-specific expression of a fluorescent reporter driven by the alphaA crystallin promoter. Fetal fibroblasts were isolated from transgenic fetuses, reprogrammed to iPS cells, and differentiated to lentoid bodies exploiting the specific fluorescence of the lens cell-specific reporter. The employment of cell type-specific reporters for establishing and optimizing differentiation in vitro seems to be an efficient and generally applicable approach for developing differentiation protocols for desired cell populations.
Friedrich-Loeffler-Institut, Institut für Nutztiergenetik, Neustadt, Germany.|ICAR-National Research Center on Equines, Hisar, India.
Taruna
T
Anand
Friedrich-Loeffler-Institut, Institut für Nutztiergenetik, Neustadt, Germany.|ICAR-National Research Center on Equines, Hisar, India.
Thirumala R
TR
Talluri
Friedrich-Loeffler-Institut, Institut für Nutztiergenetik, Neustadt, Germany.|ICAR-Central Institute for Research on Buffalos, Hisar, India.
Dharmendra
D
Kumar
Medical School Hannover, Hannover, Germany.
Wiebke
W
Garrels
Friedrich-Loeffler-Institut, Institut für Nutztiergenetik, Neustadt, Germany.
Ayan
A
Mukherjee
German Primate Center, Göttingen, Germany.
Katharina
K
Debowski
German Primate Center, Göttingen, Germany.
Rüdiger
R
Behr
Friedrich-Loeffler-Institut, Institut für Nutztiergenetik, Neustadt, Germany.
Wilfried A
http://orcid.org/0000-0002-0850-8103
WA
Kues
D000818:Animals; D002454:Cell Differentiation; D065150:Cellular Reprogramming; D003459:Crystallins / Q000378:metabolism; D004251:DNA Transposable Elements / Q000235:genetics; D005260:Female; D005333:Fetus / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D005453:Fluorescence; D017930:Genes, Reporter; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D007908:Lens, Crystalline / Q000166:cytology*; D051379:Mice; D009928:Organ Specificity
D003459:Crystallins; D004251:DNA Transposable Elements