Actin depolymerization-induced changes in proteome of Arabidopsis roots.
Actin cytoskeleton is a vital cellular structure primarily known for controlling cell integrity, division and expansion. Here we present a proteomic dissection of Arabidopsis roots treated by actin depolymerizing agent latrunculin B. Pharmacological disintegration of the actin cytoskeleton by latrunculin B caused downregulation of several proteins involved in the actin organization and dynamics. Moreover, this approach helped to identify new protein candidates involved in gene transcription, due to the altered abundance of proteins involved in mRNA nuclear export. Finally, latrunculin B negatively affected the abundance of abscisic acid (ABA) responsive proteins.



This article substantially contributes to the current knowledge about the importance of actin organization and dynamics in proteome remodelling. We employed gel based and gel free proteomic analyses and identified several new protein candidates and protein networks linking actin dynamics to the gene transcription and to the ABA response in Arabidopsis.
Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, 783 71 Olomouc, Czech Republic.
Tomáš
T
Takáč
Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, 783 71 Olomouc, Czech Republic.
Slávka
S
Bekešová
Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, 783 71 Olomouc, Czech Republic. Electronic address: jozef.samaj@upol.cz.
Jozef
J
Šamaj
D000040:Abscisic Acid / Q000502:physiology; D008841:Actin Cytoskeleton / Q000187:drug effects; D000199:Actins / Q000737:chemistry* / Q000378:metabolism; D017360:Arabidopsis / Q000737:chemistry*; D029681:Arabidopsis Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry*; D058105:Polymerization / Q000187:drug effects; D020543:Proteome / Q000032:analysis / Q000187:drug effects*; D040901:Proteomics / Q000379:methods; D053778:Thiazolidines / Q000494:pharmacology
D000199:Actins; D029681:Arabidopsis Proteins; D019086:Bridged Bicyclo Compounds, Heterocyclic; D020543:Proteome; D053778:Thiazolidines; D000040:Abscisic Acid; C037068:latrunculin B
ABA responsive proteins; Actin cytoskeleton; Arabidopsis root; Latrunculin B; Proteomics; mRNA export