Differential Expression of TGF-β Isoforms in Human Kerationocytes by Narrow Band UVB.
BACKGROUND
Transforming growth factor-β (TGF-β), a multifunctional growth factor, has three isoforms: TGF-β1, TGF-β2, and TGF-β3. Different isoforms of TGF-β are associated with different proliferation and differentiation states of the epidermis. Narrow band ultraviolet B (NBUVB) emits a concentrated UVB source of 311 nm. NBUVB 1,000 mJ/cm(2) induces apoptosis in approximately 50% of keratinocytes.


OBJECTIVE
The purpose of this study was to evaluate whether irradiation with NBUVB would alter the expression and production of TGF-β1, 2, and 3.


METHODS
We measured TGF-β1, 2, and 3 mRNA and TGF-β1 and 2 protein levels at 800, 1,000, and 1,200 mJ/cm(2) for 24 hours and 48 hours.


RESULTS
TGF-β1 mRNA levels were increased at both 24 hr and 48 hr, TGF-β2 mRNA levels were decreased at both 24 hr and 48 hr, and TGF-β3 mRNA levels were increased at 24 hr and similar to control at 48 hr. TGF-β1 protein levels were increased at 48 hr but decreased at 24 hr. TGF-β2 protein levels were decreased at both 24 hr and 48 hr.


CONCLUSIONS
The results suggest a possible role for TGF-β1 after NBUVB irradiation and opposing roles for TGF-β1 and TGF-β2 isoforms in NBUVB irradiation.
Department of Dermatology, School of Medicine, Kyung Hee University, Seoul, Korea.
Moon Chul
MC
Jung
Department of Dermatology, School of Medicine, Kyung Hee University, Seoul, Korea.
Min Kyung
MK
Shin
Department of Dermatology, School of Medicine, Kyung Hee University, Seoul, Korea.
Kyung Kook
KK
Hong
Department of Dermatology, School of Medicine, Kyung Hee University, Seoul, Korea.
Ki Heon
KH
Jeong
Department of Dermatology, School of Medicine, Kyung Hee University, Seoul, Korea.
Nack In
NI
Kim
Keratinocytes; NBUVB; Transforming growth factor β (TGF-β)