A Non-radioassay of Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase by Anion-Exchange Chromatography.
A non-radioisotopic assay method for ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is described. 3-Phosphoglycerate produced by the RuBisCO reaction was measured using an anion-exchange chromatography. The reaction course of spinach RuBisCO measured by this method was completely the same as that measured with (14)CO2.
a Plant Molecular Physiology Laboratory, Research Institute of Innovative Technology for the Earth (RITE).
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Uemura
a Plant Molecular Physiology Laboratory, Research Institute of Innovative Technology for the Earth (RITE).
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Shimoide
a Plant Molecular Physiology Laboratory, Research Institute of Innovative Technology for the Earth (RITE).|b Graduate School of Biological Sciences, Nara Institute of Science and Technology (NAIST).
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Yokota
anion-exchange chromatography; assay; phosphoglycerate; ribulose 1,5-bisphosphate; ribulose 1,5-bisphosphate carboxylase/oxygenase