Investigation of 1377C/T polymorphism of the Toll-like receptor 3 among patients with chronic hepatitis B.
The immunopathogenesis of chronic hepatitis B (CHB) has not been clarified yet. Toll-like receptors (TLR) are a receptor family that initiates immunity with exogenous-endogenous ligands and plays a role in the pathogenesis of infections. In this study, we aimed to investigate the frequency of TLR 3 1377C/T (rs3775290) polymorphism and its role in patients with CHB. We included 50 healthy individuals as control group and 73 active and 43 inactive hepatitis B patients. All DNA samples were isolated from blood samples. For the detection of TLR 3 1377C/T single-nucleotide polymorphism, restriction fragment length polymorphism was used. A statistically significant difference was determined in Hepatitis B virus (HBV) DNA levels of CHB patients with the CC, CT, and TT genotypes (p = 0.013). The highest levels of HBV DNA were detected in individuals with TT genotypes. Additionally, the frequency of CC genotype was higher in the active CHB patients compared with that of the inactive CHB patients (p = 0.044). No statistically significant difference in TLR 3 1377C/T polymorphism was detected between healthy controls and the hepatitis B patients (p = 0.342). In conclusion, HBV DNA level was higher in the individuals with TT genotype, and CC genotype was more frequent in the active CHB patients. These results suggest a possible association between CHB and TLR 3 gene (1377C/T) polymorphism.
a Kecioren Training and Research Hospital, Clinic of Infectious Diseases and Clinical Microbiology, Ankara, Turkey.
Emine Firat
EF
Goktas
b Ankara Training and Research Hospital, Department of Infectious Diseases and Clinical Microbiology, Ankara, Turkey.
Cemal
C
Bulut
c Yıldırım Beyazit University, Faculty of Medicine, Department of Medical Pharmacology, Ankara, Turkey.
Mustafa Tugrul
MT
Goktas
d Selcuk University, Faculty of Medicine, Department of Medical Pharmacology, Konya, Turkey.
Erdem Kamil
EK
Ozer
e Hacettepe University, Faculty of Medicine, Department of Medical Pharmacology, Ankara, Turkey.
Ragip Ozgur
RO
Karaca
b Ankara Training and Research Hospital, Department of Infectious Diseases and Clinical Microbiology, Ankara, Turkey.
Sami
S
Kinikli
b Ankara Training and Research Hospital, Department of Infectious Diseases and Clinical Microbiology, Ankara, Turkey.
Ali Pekcan
AP
Demiroz
f BAU International University Batumi, Faculty of Medicine, Department of Medical Pharmacology, Batumi, Georgia.
Atilla
A
Bozkurt
D000328:Adult; D000368:Aged; D005260:Female; D006515:Hepatitis B virus / Q000276:immunology; D019694:Hepatitis B, Chronic / Q000821:virology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D051196:Toll-Like Receptor 3 / Q000235:genetics*
C495344:TLR3 protein, human; D051196:Toll-Like Receptor 3
Toll-like receptors; chronic hepatitis B; hépatite B chronique; polymorphisme mononucléotidique; récepteurs de type Toll; single-nucleotide polymorphism