Antioxidant and renoprotective effects of sphingosylphosphorylcholine on contrast-induced nephropathy in rats.
Contrast induced nephropathy (CIN) is a major cause of morbidity, and increased costs as well as an increased risk of death. This study was evaluated effects of exogenous sphingosylphosphorylcholine (SPC) administration on CIN in rats. Eight animals were included in each of the following eight groups: control, control phosphate-buffered solution (PBS), control SPC 2, control SPC 10, CIN, CIN PBS, CIN SPC 2 and CIN SPC 10. The induced nephropathy was created by injected with 4 g iodine/kg body weight. SPC was administered 3 d at a daily two different doses of 2 μm/mL and 10 μm/mL intraperitoneally. The severity of renal injury score was determined by the histological and immunohistochemical changes in the kidney. Malondialdehyde (MDA), nitric oxide (NO) and superoxide dismutase (SOD) were determined to evaluate the oxidative status in the renal tissue. Treatment with 2 and 10 μM SPC inhibited the increase in renal MDA, NO levels significantly and also attenuated the depletion of SOD in the renal injuryCIN. These data were supported by histopathological findings. The inducible nitric oxide synthase positive cells and apoptotic cells in the renal tissue were observed to be reduced with the 2 and 10 μM SPC treatment. These findings suggested that 2 and 10 μM doses can attenuate renal damage in contrast nephropathy by prevention of oxidative stress and apoptosis. The low and high dose SPC may be a promising new therapeutic agent for CIN.
a Department of Cardiology , Istanbul Medeniyet University, Goztepe Training and Research Hospital , Istanbul , Turkey ;
Feyza
F
Aksu
b Department of Pediatric Surgery , Istanbul Medeniyet University, Goztepe Training and Research Hospital , Istanbul , Turkey ;
Burhan
B
Aksu
c Department of Anesthesiology and Reanimation , Edirne State Hospital , Edirne , Turkey ;
Nermin
N
Unlu
d Department of Histology and Embryology, Faculty of Medicine , Trakya University , Edirne , Turkey ;
Turan
T
Karaca
e Department of Pediatric Surgery, Faculty of Medicine , Trakya University , Edirne , Turkey ;
Suleyman
S
Ayvaz
f Department of Biochemistry , Istanbul Medeniyet University, Goztepe Training and Research Hospital , Istanbul , Turkey ;
Hayriye
H
Erman
g Department of Biochemistry, Cerrahpasa Medical Faculty , Istanbul University , Istanbul , Turkey ;
Hafize
H
Uzun
a Department of Cardiology , Istanbul Medeniyet University, Goztepe Training and Research Hospital , Istanbul , Turkey ;
Nursen
N
Keles
h Department of Physiology , Marmara University, Pendik Education and Research Hospital , Istanbul , Turkey ;
Sule
S
Bulur
i Department of Radiodiagnostics, Faculty of Medicine , Trakya University , Edirne , Turkey.
Ercument
E
Unlu
D058186:Acute Kidney Injury / Q000139:chemically induced* / Q000188:drug therapy*; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D003287:Contrast Media / Q000009:adverse effects*; D003404:Creatinine / Q000097:blood; D006801:Humans; D007274:Injections, Intraperitoneal; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008315:Malondialdehyde / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010767:Phosphorylcholine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D013110:Sphingosine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000627:therapeutic use; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D003287:Contrast Media; C005356:sphingosine phosphorylcholine; D010767:Phosphorylcholine; D009569:Nitric Oxide; D008315:Malondialdehyde; D003404:Creatinine; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; D013482:Superoxide Dismutase; D013110:Sphingosine
Contrast-induced nephropathy; nitric oxide; oxidative stress; renal injury; sphingosylphosphorylcholine