OsWRKY51, a rice transcription factor, functions as a positive regulator in defense response against Xanthomonas oryzae pv. oryzae.
OsWRKY51 functions as a positive transcriptional regulator in defense signaling against Xanthomonas oryzae pv. oryzae by direct DNA binding to the promoter of defense related gene, OsPR10a. OsWRKY51 in rice (Oryza sativa L.) is induced by exogenous salicylic acid (SA) and inoculation with Xanthomonas oryzae pv. oryzae (Xoo). To examine the role of OsWRKY51 in the defense response of rice, we generated OsWRKY51 overexpressing and underexpressing transgenic rice plants. OsWRKY51-overexpressing transgenic rice lines were more resistant to Xoo and showed greater expression of defense-related genes than wild-type (WT) plants, while OsWRKY51-underexpressing lines were more susceptible to Xoo and showed less expression of defense-associated genes than WT plants. Transgenic lines overexpressing OsWRKY51 showed growth retardation compared to WT plants. In contrast, transgenic lines underexpressing OsWRKY51 by RNA interference showed similar plant height with WT plants. Transient expression of OsWRKY51-green fluorescent protein fusion protein in rice protoplasts revealed that OsWRKY51 was localized in the nucleus. OsWRKY51 bound to the W-box and WLE1 elements of the OsPR10a promoter. Based on these results, we suggest that OsWRKY51 is a positive transcriptional regulator of defense signaling and has direct DNA binding ability to the promoter of OsPR10a, although it is reported to be a negative regulator in GA signaling.
National Institute of Agricultural Science, Rural Development Administration, Jeonju, 560-500, Korea.
Seon-Hee
SH
Hwang
National Institute of Agricultural Science, Rural Development Administration, Jeonju, 560-500, Korea.|Convergence Research Center for Functional Plant Products, Advanced Institutes of Convergence Technology (AICT), Suwon, 443-270, Korea.
Soon Il
SI
Kwon
National Institute of Agricultural Science, Rural Development Administration, Jeonju, 560-500, Korea.
Ji-Young
JY
Jang
National Institute of Agricultural Science, Rural Development Administration, Jeonju, 560-500, Korea.
Il Lan
IL
Fang
National Institute of Agricultural Science, Rural Development Administration, Jeonju, 560-500, Korea.
Heyoung
H
Lee
National Institute of Agricultural Science, Rural Development Administration, Jeonju, 560-500, Korea.
Changhyun
C
Choi
National Institute of Agricultural Science, Rural Development Administration, Jeonju, 560-500, Korea.
Sangryeol
S
Park
National Institute of Agricultural Science, Rural Development Administration, Jeonju, 560-500, Korea.
Ilpyung
I
Ahn
National Institute of Agricultural Science, Rural Development Administration, Jeonju, 560-500, Korea.
Shin-Chul
SC
Bae
National Institute of Agricultural Science, Rural Development Administration, Jeonju, 560-500, Korea. djhwang@rda.go.kr.
Duk-Ju
DJ
Hwang
D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D055785:Gene Knockdown Techniques; D008954:Models, Biological; D012275:Oryza / Q000187:drug effects / Q000254:growth & development / Q000276:immunology* / Q000382:microbiology*; D010641:Phenotype; D010935:Plant Diseases / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D011485:Protein Binding / Q000187:drug effects; D034622:RNA Interference; D020156:Salicylic Acid / Q000494:pharmacology; D013347:Subcellular Fractions / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014974:Xanthomonas / Q000187:drug effects / Q000502:physiology*
D010940:Plant Proteins; D014157:Transcription Factors; D020156:Salicylic Acid
DNA binding; Defense; OsWRKY51; Xanthomonas oryzae pv. oryzae