Fullerenol Nanoparticles with Structural Activity Induce Variable Intracellular Actin Filament Morphologies.
Fullerenol nanoparticles are promising for various biological applications; many studies have shown that they induce variable and diverse biological effects including side effects. Separation and purification of two fractions of fullerenols has demonstrated that they have varied chemical structures on the surfaces of their carbon cages. Actin is an important structural protein that is able to transform functional structures under varied physiological conditions. We assessed the abilities of the two fractions of fullerenols to attach to actin and induce variable morphological features in actin filament structures. Specifically the fullerenol fraction with a surface electric charge of -1.913 ± 0.008q (x10(-6) C) has percentages of C-OH and C=O on the carbon cage of 16.14 ± 0.60 and 17.55 ± 0.69. These features allow it to form intermolecular hydrogen bonds with actin at a stoichiometric ratio of four fullerenols per actin subunit. Molecular simulations revealed these specific binding sites and binding modes in atomic details in the interaction between the active fullerenol and actin filament. Conversely, these interactions were not possible for the other fraction of fullerenol with that percentages of C-OH and C=O on the carbon cage were 15.59 ± 0.01 and 1.94 ± 0.11. Neither sample induced appreciable cytotoxicity or acute cell death. After entering cells, active fullerenol binding to actin induces variable morphological features and may transform ATP-actin to ADP-actin. These changes facilitate the binding of ADF/cofilin, allowing cofilin to sever actin filaments to form cofilin/actin/fullerenol rods. Our findings suggest that fullerenol with structural activity binding disturbs actin filament structure, which may inhibit locomotion of cell or induce chronic side effects in to cells.
Junjiang
J
Jin
Ying
Y
Dong
Ying
Y
Wang
Lin
L
Xia
Weihong
W
Gu
Xue
X
Bai
Yanan
Y
Chang
Mingyi
M
Zhang
Kui
K
Chen
Juan
J
Li
Lina
L
Zhao
Gengmei
G
Xing
D008841:Actin Cytoskeleton / Q000737:chemistry* / Q000378:metabolism; D051339:Actin Depolymerizing Factors / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D037741:Fullerenes / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D051379:Mice; D056004:Molecular Dynamics Simulation; D053758:Nanoparticles / Q000737:chemistry* / Q000378:metabolism
D051339:Actin Depolymerizing Factors; D037741:Fullerenes; C108127:fullerenol