Influence of Oleic Acid on Rumen Fermentation and Fatty Acid Formation In Vitro.
A series of batch cultures were conducted to investigate the effects of oleic acid (OA) on in vitro ruminal dry matter degradability (IVDMD), gas production, methane (CH4) and hydrogen (H2) production, and proportion of fatty acids. Rumen fluid was collected from fistulated goats, diluted with incubation buffer, and then incubated with 500 mg Leymus chinensis meal supplemented with different amounts of OA (0, 20, 40, and 60 mg for the CON, OA20, OA40 and OA60 groups, respectively). Incubation was carried out anaerobically at 39°C for 48 h, and the samples were taken at 12, 24 and 48 h and subjected to laboratory analysis. Supplementation of OA decreased IVDMD, the cumulative gas production, theoretical maximum of gas production and CH4 production, but increased H2 production. However, no effect was observed on any parameters of rumen fermentation (pH, ammonia, production of acetate, propionate and butyrate and total volatile fatty acid production). The concentrations of some beneficial fatty acids, such as cis monounsaturated fatty acids and conjugated linoleic acid (CLA) were higher (P < 0.05) from OA groups than those from the control group at 12 h incubation. In summary, these results suggest that the OA supplementation in diet can reduce methane production and increase the amount of some beneficial fatty acids in vitro.
Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, P.R. China.|Institute of bast fiber crops, Chinese Academy of Agricultrial Sciences, Changsha, Hunan 410205, P.R. China.
Duanqin
D
Wu
Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, P.R. China.
Liwei
L
Xu
Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, P.R. China.
Shaoxun
S
Tang
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada.
Leluo
L
Guan
Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, P.R. China.
Zhixiong
Z
He
UWA Institute of Agriculture M082, University of Western Australia, 35 Stirling Highway, Crawley WA 6009, Australia.
Yongjuan
Y
Guan
Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, P.R. China.
Zhiliang
Z
Tan
Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, P.R. China.
Xuefeng
X
Han
Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, P.R. China.
Chuanshe
C
Zhou
Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, P.R. China.
Jinhe
J
Kang
Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan 410125, P.R. China.
Min
M
Wang
D000085:Acetates / Q000378:metabolism; D000641:Ammonia / Q000378:metabolism; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D019587:Dietary Supplements / Q000032:analysis; D005227:Fatty Acids / Q000378:metabolism*; D005232:Fatty Acids, Volatile / Q000378:metabolism; D005285:Fermentation; D006041:Goats / Q000502:physiology*; D006859:Hydrogen / Q000378:metabolism; D008697:Methane / Q000378:metabolism; D019301:Oleic Acid / Q000378:metabolism*; D011422:Propionates / Q000378:metabolism; D012417:Rumen / Q000502:physiology*
D000085:Acetates; D005227:Fatty Acids; D005232:Fatty Acids, Volatile; D011422:Propionates; D019301:Oleic Acid; D000641:Ammonia; D006859:Hydrogen; D008697:Methane