Cytokines profile and its correlation with endothelial damage and oxidative stress in patients with type 1 diabetes mellitus and nephropathy.
The aim of the present study was to investigate the association between the presence of albuminuria and cytokines profile with biomarkers of endothelial damage and oxidative stress in patients with type 1 diabetes mellitus (DM1). The sample was composed by 35 healthy individuals, 63 DM1 patients with normoalbuminuria (<30 mg of albumin/g of creatinine) and 62 DM1 patients with micro- and macroalbuminuria (≥30 mg of albumin/g of creatinine). Plasma and urinary cytokines (TNF-α, IL-6 and IL-10) and thrombomodulin levels were determined by ELISA. Oxidative status was evaluated using the TBARS and MTT assays. Diabetic patients were characterized by elevated levels of urinary cytokines TNF-α, IL-6 and IL-10. Those with macroalbuminuria presented significantly higher TNF-α and IL-10 urinary levels when compared to other groups. Urinary and plasmatic levels of TNF-α were positively correlated with plasma levels of cystatin C, creatinine, urea and albuminuria, while they were negatively correlated with estimated glomerular filtration rate. Urinary IL-10 levels proved positive correlation with fasting glucose, HbA1c, thrombomodulin and TBARS, while IL-6 plasma levels were positively correlated with HbA1c and albuminuria. Only urinary TNF-α levels were associated with the presence and severity of macroalbuminuria, after logistic regression analysis. This finding suggests that measurement of urinary TNF-α level may be helpful to evaluate progression to nephropathy in DM1 patients.
Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal de Minas Gerais (UFMG), Av Antonio Carlos, 6627, Pampulha, CEP: 31270-901, Belo Horizonte, MG, Brazil.
Rodrigo M C
RM
Pestana
Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal de Minas Gerais (UFMG), Av Antonio Carlos, 6627, Pampulha, CEP: 31270-901, Belo Horizonte, MG, Brazil.
Caroline P
CP
Domingueti
Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal de Minas Gerais (UFMG), Av Antonio Carlos, 6627, Pampulha, CEP: 31270-901, Belo Horizonte, MG, Brazil.
Rita C F
RC
Duarte
Departamento de Clínica Médica, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
Rodrigo B
RB
Fóscolo
Instituto de Ensino e Pesquisa da Santa Casa de Belo Horizonte, Belo Horizonte, MG, Brazil.
Janice S
JS
Reis
Faculdade de Enfermagem, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
Ana Maria S
AM
Rodrigues
Faculdade de Enfermagem, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
Laís B
LB
Martins
Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal de Minas Gerais (UFMG), Av Antonio Carlos, 6627, Pampulha, CEP: 31270-901, Belo Horizonte, MG, Brazil.
Lirlândia P
LP
Sousa
Colégio Técnico, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
Daniela P
DP
Lage
Colégio Técnico, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
Cláudia N
CN
Ferreira
Faculdade de Enfermagem, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
Adaliene V M
AV
Ferreira
Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal de Minas Gerais (UFMG), Av Antonio Carlos, 6627, Pampulha, CEP: 31270-901, Belo Horizonte, MG, Brazil.
Ana P
AP
Fernandes
Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal de Minas Gerais (UFMG), Av Antonio Carlos, 6627, Pampulha, CEP: 31270-901, Belo Horizonte, MG, Brazil. karinabgb@gmail.com.
Karina B
KB
Gomes
D000328:Adult; D000419:Albuminuria; D015415:Biomarkers / Q000097:blood / Q000652:urine*; D055316:Cystatin C / Q000378:metabolism; D003922:Diabetes Mellitus, Type 1 / Q000175:diagnosis / Q000276:immunology*; D018450:Disease Progression; D004727:Endothelium / Q000378:metabolism*; D005260:Female; D005919:Glomerular Filtration Rate; D006801:Humans; D016753:Interleukin-10 / Q000097:blood / Q000652:urine; D015850:Interleukin-6 / Q000097:blood / Q000652:urine; D007668:Kidney / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000652:urine*; D055815:Young Adult
D015415:Biomarkers; D055316:Cystatin C; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10
Albuminuria; Cytokines; Endothelial damage; Oxidative stress; Type 1 diabetes mellitus