[Effect of Electroacupuncture Combined with Polysaccharide of Gastrodia elata Blume on Ex- pression of Brain Derived Neurotrophic Factor and Vascular Endothelial Growth Factor in the Paraventricular Nucleus of Hypothalamus in Cerebral Ischemia Rat].
OBJECTIVE
To observe the effect of electroacupuncture(EA) of "Baihui" (GV 20) and "Zusanli" (ST 36) in combination with administration of polysaccharide of Gastrodia elata Blume (PGB) on the expression of brain derived neurotrophic factor (BDNF) and vascular endothelial growth factor(VEGF) in the paraventricular nucleus of hypothalamus(PVN) of cerebral ischemia (C) rats, so as to explore its mechanism underlying improvement of Cl.


METHODS
Forty Sprague-Dawley rats were randomized into five groups: normal control, model, EA, PGB, and EA+ PGB (n = 8/group). The cerebral ischemia model was established by occlusion of the middle cerebral artery (MCAO). EA (2 Hz, 2 V) was applied to "Baihui" (GV 20) and "Zusanli" (ST 36) for 30 min, once daily for 14 days. The rats of the PGB and PGB+ EA groups were treated by intragastric gavage of PGB at a dose of 100 mg/kg, once daily for 14 successive days. The expression of BDNF and VEGF in the PVN of hypothalamus was detected by immunohistochemistry.


RESULTS
After MCAO, the BDNF and VEGF immunoreactions (IR) positive neuron numbers and expression level in the PVN of the hypothalamus were significantly increased in the model group (P<0.05) and were further up-regulated after administration of EA and PBG ( P<0. 05). The effects of EA+ PGB were evidently superior to those of simple EA and simple PBG in up-regulating BDNF and VEGF IR-positive neuron numbers and expression levels in the PVN ( P<0.05).


CONCLUSIONS
EA combined with PGB can up-regulate the expression of BDNF and VEGF in the PVN of hypothalamus in cerebral ischemia rats, which might contribute to its effect in improving cerebral ischemia.
Min
M
Liu
Hua-chun
HC
Miao
Huai-bin
HB
Li
Jian
J
Zhao
Ke-ren
KR
Xiong
D000818:Animals; D002545:Brain Ischemia / Q000235:genetics / Q000378:metabolism / Q000628:therapy*; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics* / Q000378:metabolism; D003131:Combined Modality Therapy; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D015671:Electroacupuncture; D031668:Gastrodia / Q000737:chemistry*; D006801:Humans; D008297:Male; D010286:Paraventricular Hypothalamic Nucleus / Q000187:drug effects / Q000378:metabolism*; D011134:Polysaccharides / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics* / Q000378:metabolism
D019208:Brain-Derived Neurotrophic Factor; D004365:Drugs, Chinese Herbal; D011134:Polysaccharides; D042461:Vascular Endothelial Growth Factor A