Sequence-specific detection of cytosine methylation in DNA via the FRET mechanism between upconversion nanoparticles and gold nanorods.
A general detection method for DNA methylation is developed based on the FRET mechanism between upconversion nanoparticles and gold nanorods, which can recognize the cytosine methylation in a known DNA strand at a concentration as low as 7 pM.
State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, P. R. China. zguo@nju.edu.cn.
Mingmin
M
Wu
State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, P. R. China. boxwxy@nju.edu.cn.
Xiaoyong
X
Wang
State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, P. R. China. zguo@nju.edu.cn.
Kun
K
Wang
State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, P. R. China. zguo@nju.edu.cn.
Zijian
Z
Guo
D001483:Base Sequence; D003596:Cytosine / Q000378:metabolism*; D004247:DNA / Q000737:chemistry / Q000235:genetics*; D019175:DNA Methylation; D031541:Fluorescence Resonance Energy Transfer; D006046:Gold / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D043942:Nanotubes / Q000737:chemistry*; D009690:Nucleic Acid Conformation
D006046:Gold; D003596:Cytosine; D004247:DNA