Development of quantitative mass spectrometric immunoassay for serum amyloid A.
OBJECTIVE
Proteins can exist as multiple proteoforms in vivo that can have important roles in physiological and pathological states.


METHODS
We present the development and characterization of mass spectrometric immunoassay (MSIA) for quantitative determination of serum amyloid A (SAA) proteoforms.


RESULTS
Intra- and inter-day precision revealed CVs <10%. Against existing SAA ELISA, the developed MSIA showed good correlation according to the Altman-Bland plot. Individual concentrations of the SAA proteoforms across a cohort of 170 samples revealed 7 diverse SAA polymorphic types and 12 different proteoforms.


CONCLUSIONS
The new SAA MSIA enables parallel analysis of SAA polymorphisms and quantification of all expressed SAA proteoforms, in a high-throughput and time-efficient manner.
a The Biodesign Institute, Arizona State University , Tempe , AZ , USA.
Olgica
O
Trenchevska
b Department of Medicine , University of Southern California , Los Angeles , CA , USA.
Hussein N
HN
Yassine
a The Biodesign Institute, Arizona State University , Tempe , AZ , USA.
Chad R
CR
Borges
a The Biodesign Institute, Arizona State University , Tempe , AZ , USA.
Randall W
RW
Nelson
a The Biodesign Institute, Arizona State University , Tempe , AZ , USA.
Dobrin
D
Nedelkov
Baseline concentration; inflammation biomarker; mass spectrometry; polymorphism; posttranslational modifications; proteoforms