Genetic assessment of meiobenthic community composition and spatial distribution in coastal sediments along northern Gulf of Mexico.
Meiobenthic (meiofauna and micro-eukaryotes) organisms are important contributors to ecosystem functioning in aquatic environments through their roles in nutrient transport, sediment stability, and food web interactions. Despite their ecological importance, information pertaining to variation of these communities at various spatial and temporal scales is not widely known. Many studies in the Gulf of Mexico (GOM) have focused either on deep sea or continental shelf areas, while little attention has been paid to bays and coastal regions. Herein, we take a holistic approach by using high-throughput sequencing approaches to examine spatial variation in meiobenthic communities within Alabama bays and the coastal northern GOM region. Sediment samples were collected along three transects (Mississippi Sound: MS, FOCAL: FT, and Orange Beach: OB) from September 2010 to April 2012 and community composition was determined by metabarcoding the V9 hypervariable region of the nuclear18S rRNA gene. Results showed that Stramenopiles (diatoms), annelids, arthropods (copepods), and nematodes were the dominate groups within samples, while there was presence of other phyla throughout the dataset. Location played a larger role than time sampled in community composition. However, samples were collected over a short temporal scale. Samples clustered in reference to transect, with the most eastern transect (OB) having a distinct community composition in comparison to the other two transects (MS and FT). Communities also differed in reference to region (Bay versus Shelf). Bulk density and percent inorganic carbon were the only measured environmental factors that were correlated with community composition.
Department of Biological Sciences, Auburn University, 101 Rouse Life Science Building, Auburn, AL, 36849, USA. Electronic address: pamela.m.brannock@gmail.com.
Pamela M
PM
Brannock
Department of Marine Sciences, University of South Alabama, 307 University Blvd, Mobile, AL, 36688, USA; Dauphin Island Sea Lab, 101B Bienville Blvd, Dauphin Island, AL, 36528, USA.
Lei
L
Wang
Department of Marine Sciences, University of South Alabama, 307 University Blvd, Mobile, AL, 36688, USA; Dauphin Island Sea Lab, 101B Bienville Blvd, Dauphin Island, AL, 36528, USA.
Alice C
AC
Ortmann
Department of Biological Sciences, Auburn University, 101 Rouse Life Science Building, Auburn, AL, 36849, USA.
Damien S
DS
Waits
Department of Biological Sciences, Auburn University, 101 Rouse Life Science Building, Auburn, AL, 36849, USA. Electronic address: ken@auburn.edu.
Kenneth M
KM
Halanych
D000818:Animals; D059001:Aquatic Organisms / Q000145:classification / Q000235:genetics*; D017753:Ecosystem; D004784:Environmental Monitoring; D019015:Geologic Sediments / Q000737:chemistry; D060851:Gulf of America; D007448:Invertebrates / Q000145:classification / Q000235:genetics*
18S rRNA; High-throughput sequencing; Illumina; Meiofauna; Metabarcoding; Micro-eukaryotes; Protists