Experimental Models in Syrian Golden Hamster Replicate Human Acute Pancreatitis.
The hamster has been shown to share a variety of metabolic similarities with humans. To replicate human acute pancreatitis with hamsters, we comparatively studied the efficacy of common methods, such as the peritoneal injections of caerulein, L-arginine, the retrograde infusion of sodium taurocholate, and another novel model with concomitant administration of ethanol and fatty acid. The severity of pancreatitis was evaluated by serum amylase activity, pathological scores, myeloperoxidase activity, and the expression of inflammation factors in pancreas. The results support that the severity of pathological injury is consistent with the pancreatitis induced in mice and rat using the same methods. Specifically, caerulein induced mild edematous pancreatitis accompanied by minimal lung injury, while L-arginine induced extremely severe pancreatic injury including necrosis and neutrophil infiltration. Infusion of Na-taurocholate into the pancreatic duct induced necrotizing pancreatitis in the head of pancreas and lighter inflammation in the distal region. The severity of acute pancreatitis induced by combination of ethanol and fatty acids was between the extent of caerulein and L-arginine induction, with obvious inflammatory cells infiltration. In view of the advantages in lipid metabolism features, hamster models are ideally suited for the studies of pancreatitis associated with altered metabolism in humans.
Institute of Cardiovascular Science, Key laboratory of Molecular Cardiovascular Science of Ministry of Education, Health Science Center, Peking University, Beijing, 100191, China.
Yunan
Y
Wang
Institute of Cardiovascular Science, Key laboratory of Molecular Cardiovascular Science of Ministry of Education, Health Science Center, Peking University, Beijing, 100191, China.
Abudurexiti
A
Kayoumu
Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing, 210093, China.
Guotao
G
Lu
Institute of Cardiovascular Science, Key laboratory of Molecular Cardiovascular Science of Ministry of Education, Health Science Center, Peking University, Beijing, 100191, China.
Pengfei
P
Xu
Institute of Cardiovascular Science, Key laboratory of Molecular Cardiovascular Science of Ministry of Education, Health Science Center, Peking University, Beijing, 100191, China.
Xu
X
Qiu
Institute of Cardiovascular Science, Key laboratory of Molecular Cardiovascular Science of Ministry of Education, Health Science Center, Peking University, Beijing, 100191, China.
Liye
L
Chen
Institute of Cardiovascular Science, Key laboratory of Molecular Cardiovascular Science of Ministry of Education, Health Science Center, Peking University, Beijing, 100191, China.
Rong
R
Qi
University of Cincinnati College of Medicine, Cincinnati, OH, USA.
Shouxiong
S
Huang
Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing, 210093, China.
Weiqin
W
Li
Institute of Cardiovascular Science, Key laboratory of Molecular Cardiovascular Science of Ministry of Education, Health Science Center, Peking University, Beijing, 100191, China.
Yuhui
Y
Wang
Institute of Cardiovascular Science, Key laboratory of Molecular Cardiovascular Science of Ministry of Education, Health Science Center, Peking University, Beijing, 100191, China.
George
G
Liu
D000681:Amylases / Q000097:blood; D000818:Animals; D001120:Arginine / Q000009:adverse effects*; D002108:Ceruletide / Q000009:adverse effects*; D004195:Disease Models, Animal; D000431:Ethanol / Q000009:adverse effects*; D005227:Fatty Acids / Q000009:adverse effects*; D006801:Humans; D007267:Injections; D008647:Mesocricetus; D010195:Pancreatitis / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology*; D009195:Peroxidase / Q000378:metabolism; D012720:Severity of Illness Index; D013656:Taurocholic Acid / Q000009:adverse effects*
D005227:Fatty Acids; D000431:Ethanol; D013656:Taurocholic Acid; D002108:Ceruletide; D001120:Arginine; D009195:Peroxidase; D000681:Amylases