Enzyme coated beta-cyclodextrin for effective adsorption and glucose-responsive closed-loop insulin delivery.
Inconsistent dosage of insulin (INS) for type 2 diabetes patients lead to severe adverse effects like limb amputation, blindness and fatal hypo or hyper glycaemia. Hence, a drug delivery system (DDS) capable of consistent INS release by sensing changes in blood glucose level is essential. Herein, we report a glucose responsive DDS comprised of oleic acid-grafted-aminated beta cyclodextrin (OA-g-ACD) copolymer, coated with a dispersion of glucose oxidase (GOx) and catalase (CAT). The prepared DDS was characterised using FTIR, Optical Microscopy, H(1) NMR, DLS and SEM. Hydrophobicity and drug loading capacity was ascertained using contact angle measurements and confocal laser scanning microscopy (CLSM) respectively. Extent of swelling was observed to be a function of glucose concentration. INS release profile showed a cumulative release of 78.0 % after 240min. Flow cytometry studies revealed greater population of INS on HeLa cells indicating application of DDS as potential candidate for the intravenous administration of INS.
Department of Chemistry, School of Physical and Mathematical Sciences, University of Kerala, Kariavattom, Trivandrum 695 581, India. Electronic address: tsani@rediffmail.com.
T S
TS
Anirudhan
Department of Chemistry, School of Physical and Mathematical Sciences, University of Kerala, Kariavattom, Trivandrum 695 581, India.
Anoop S
AS
Nair
Department of Chemistry, School of Physical and Mathematical Sciences, University of Kerala, Kariavattom, Trivandrum 695 581, India.
Syam S
SS
Nair
D016503:Drug Delivery Systems; D005947:Glucose / Q000737:chemistry / Q000494:pharmacology; D005949:Glucose Oxidase / Q000737:chemistry / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D007328:Insulin / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D047392:beta-Cyclodextrins / Q000737:chemistry / Q000494:pharmacology
D007328:Insulin; D047392:beta-Cyclodextrins; D005949:Glucose Oxidase; D005947:Glucose; C031215:betadex
Closed loop-insulin delivery; Glucose oxidase-catalase; Hydrophobic beta cyclodextrin