The use of collagen-based matrices in the treatment of full-thickness wounds.
Chronic and complex full-thickness wounds have become increasingly prevalent. Besides autologous skin transplantation, innovative wound dressing products have gained interest, as the functional and esthetic outcome is still limited. In this respect, the effect of a novel modifiable collagen-gelatin fleece on the healing of deep dermal wounds was examined and compared with untreated controls and Matriderm(®). A total of 48 full-thickness skin defects were generated on six minipigs and treated with the novel collagen-gelatin fleece of different thicknesses in single or multiple application (n=36) or treated with Matriderm(®) in a single application (n=6), or the wounds were left untreated (n=6). Wound healing was analyzed planimetrically by wound closure per time and histologically with regard to epidermal thickness and cell density. Compared to untreated wounds, wound closure per time and histological skin quality with regard to the mean epidermal thickness and epidermal cell amount were enhanced in both treatment groups. Overall, the best results for the novel collagen-gelatin fleece were achieved for multiple applications with a thickness of 150g/m(2). The novel biomaterial shows accelerated and improved dermal wound repair in a minipig model. As the manufacturing process of the scaffold allows the integration of bioactive substances such as antibiotics and growth factors, we intend to design a composite biomaterial using this scaffold as a carrier matrix.
Department of Plastic, Reconstructive, Hand and Burn Surgery, BG-Trauma Center, Eberhard Karls University Tübingen, Tübingen, Germany.
Wiebke
W
Petersen
Department of Plastic, Hand, Reconstructive and Aesthetic Surgery, Helios Clinic Wuppertal GmbH, University Witten/Herdecke, Herdecke, Germany.
Afshin
A
Rahmanian-Schwarz
Department of Medical Oncology and Hematology, Medical Center II, University Hospital of Tuebingen, Eberhard Karls University Tübingen, Tübingen, Germany. Electronic address: jan-ole-werner@gmx.de.
Jan-Ole
JO
Werner
Department of Plastic, Reconstructive, Hand and Burn Surgery, BG-Trauma Center, Eberhard Karls University Tübingen, Tübingen, Germany.
Jennifer
J
Schiefer
Department of Plastic, Reconstructive, Hand and Burn Surgery, BG-Trauma Center, Eberhard Karls University Tübingen, Tübingen, Germany.
Jens
J
Rothenberger
Department of Plastic, Hand, Reconstructive and Aesthetic Surgery, Helios Clinic Wuppertal GmbH, University Witten/Herdecke, Herdecke, Germany.
Gunnar
G
Hübner
Department of Plastic, Reconstructive, Hand and Burn Surgery, BG-Trauma Center, Eberhard Karls University Tübingen, Tübingen, Germany.
Hans-Eberhard
HE
Schaller
Department of Plastic, Reconstructive, Hand and Burn Surgery, BG-Trauma Center, Eberhard Karls University Tübingen, Tübingen, Germany.
Manuel
M
Held
D000818:Animals; D003094:Collagen / Q000627:therapeutic use*; D020405:Dermis / Q000473:pathology; D004549:Elastin / Q000627:therapeutic use; D004817:Epidermis / Q000473:pathology; D005780:Gelatin / Q000627:therapeutic use*; D011897:Random Allocation; D013552:Swine; D013556:Swine, Miniature; D054457:Tissue Scaffolds; D014945:Wound Healing; D014947:Wounds and Injuries / Q000473:pathology / Q000628:therapy*
C532298:matriderm; D005780:Gelatin; D003094:Collagen; D004549:Elastin