Pollen DNA barcoding: current applications and future prospects.
Identification of the species origin of pollen has many applications, including assessment of plant-pollinator networks, reconstruction of ancient plant communities, product authentication, allergen monitoring, and forensics. Such applications, however, have previously been limited by microscopy-based identification of pollen, which is slow, has low taxonomic resolution, and has few expert practitioners. One alternative is pollen DNA barcoding, which could overcome these issues. Recent studies demonstrate that both chloroplast and nuclear barcoding markers can be amplified from pollen. These recent validations of pollen metabarcoding indicate that now is the time for researchers in various fields to consider applying these methods to their research programs. In this paper, we review the nascent field of pollen DNA barcoding and discuss potential new applications of this technology, highlighting existing limitations and future research developments that will improve its utility in a wide range of applications.
a Emory University, School of Environmental Sciences, Atlanta, GA, USA.
Karen L
KL
Bell
b National Botanic Garden of Wales, Llanarthne, United Kingdom.
Natasha
N
de Vere
c Department of Animal Ecology and Tropical Biology, University of Würzburg, Würzburg, Germany.
Alexander
A
Keller
d Department of Entomology, The Ohio State University, Columbus, OH, USA.
Rodney T
RT
Richardson
e Biotechnology Platform, Agricultural Research Council, Pretoria, South Africa.|f School of Life Science, University of KwaZulu-Natal, Pietermaritzburg, South Africa.
Annemarie
A
Gous
g Columbus State University, Columbus, GA, USA.
Kevin S
KS
Burgess
a Emory University, School of Environmental Sciences, Atlanta, GA, USA.
Berry J
BJ
Brosi
D000485:Allergens / Q000235:genetics / Q000276:immunology; D044822:Biodiversity; D019295:Computational Biology / Q000379:methods; D058893:DNA Barcoding, Taxonomic; D018744:DNA, Plant; D030541:Databases, Genetic; D063427:Food Quality; D005819:Genetic Markers; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D056186:Metagenomics / Q000379:methods; D010944:Plants / Q000145:classification* / Q000235:genetics*; D011058:Pollen / Q000235:genetics*
D000485:Allergens; D018744:DNA, Plant; D005819:Genetic Markers
DNA metabarcoding; high-throughput sequencing; metagenomics; métacodage à barres; métagénomique; next-generation sequencing; palynologie; palynology; pollen; séquençage de nouvelle génération; séquençage à haut débit