Methods for the Study of Gonadal Development.
Current knowledge on gonadal development and sex determination is the product of many decades of research involving a variety of scientific methods from different biological disciplines such as histology, genetics, biochemistry, and molecular biology. The earliest embryological investigations, followed by the invention of microscopy and staining methods, were based on histological examinations. The most robust development of histological staining techniques occurred in the second half of the nineteenth century and resulted in structural descriptions of gonadogenesis. These first studies on gonadal development were conducted on domesticated animals; however, currently the mouse is the most extensively studied species. The next key point in the study of gonadogenesis was the advancement of methods allowing for the in vitro culture of fetal gonads. For instance, this led to the description of the origin of cell lines forming the gonads. Protein detection using antibodies and immunolabeling methods and the use of reporter genes were also invaluable for developmental studies, enabling the visualization of the formation of gonadal structure. Recently, genetic and molecular biology techniques, especially gene expression analysis, have revolutionized studies on gonadogenesis and have provided insight into the molecular mechanisms that govern this process. The successive invention of new methods is reflected in the progress of research on gonadal development.
Department of Comparative Anatomy, Institute of Zoology, Jagiellonian University, Gronostajowa 9, 30-387, Kraków, Poland. rafal.piprek@uj.edu.pl.
Rafal P
RP
Piprek
D000818:Animals; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D018507:Gene Expression Regulation, Developmental; D006651:Histocytochemistry / Q000379:methods*; D008297:Male; D051379:Mice; D008967:Molecular Biology / Q000379:methods*; D010053:Ovary / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D013737:Testis / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*
Gene expression analysis; Gonad; Histology; Immunolabeling; In situ hybridization; In vitro culture; PCR; Reporter genes; Transgenic animals