A novel cell autolysis system for cost-competitive downstream processing.
The industrial production of low value-added biological products poses significant challenges due to cost pressures. In recent years, it has been argued that synthetic biology approaches will lead to breakthroughs that eliminate price bottlenecks for the production of a wide range of biological products including bioplastics and biofuels. One significant bottleneck lies in the necessity to break the tough cell walls of microbes in order to release intracellular products. We here report the implementation of the first synthetic biology standard part based on the lambda phage SRRz genes and a synthetic ribosome binding site (RBS) that works in Escherichia coli and Halomonas campaniensis, which enables the producer strains to induce lysis after the addition of small amounts (1-5 %) of solvents or to spontaneously lyse during the stresses of downstream processing, and thus has the potential to eliminate the mechanical cell disruption step as both an efficiency bottleneck and a significant capex barrier when implementing downstream bioprocesses.
Peking-Tsinghua Center for Life Sciences, School of Life Science, Tsinghua University, Beijing, 100084, China.
Ivan
I
Hajnal
Peking-Tsinghua Center for Life Sciences, School of Life Science, Tsinghua University, Beijing, 100084, China.
Xiangbin
X
Chen
Peking-Tsinghua Center for Life Sciences, School of Life Science, Tsinghua University, Beijing, 100084, China. chengq@mail.tsinghua.edu.cn.|Center for Synthetic and Systems Biology, Tsinghua University, Beijing, 100084, China. chengq@mail.tsinghua.edu.cn.|MOE Key Lab of Industrial Biocatalysis, Tsinghua University, Beijing, 100084, China. chengq@mail.tsinghua.edu.cn.
Guo-Qiang
GQ
Chen
D001329:Autolysis; D001433:Bacteriolysis; D010582:Bacteriophage lambda / Q000235:genetics; D001688:Biological Products / Q000302:isolation & purification*; D001709:Biotechnology / Q000191:economics / Q000379:methods*; D003365:Costs and Cost Analysis; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005814:Genes, Viral; D020632:Halomonas / Q000235:genetics / Q000378:metabolism*
D001688:Biological Products
Autolysis; Bioplastics; Halomonas; PHA; PHB; Synthetic biology