CD1c-Expression by Monocytes - Implications for the Use of Commercial CD1c+ Dendritic Cell Isolation Kits.
Conventional dendritic cells (cDCs) comprise a heterogeneous population of cells that are important regulators of immunity and homeostasis. CD1c+ cDCs are present in human blood and tissues, and found to efficiently activate naïve CD4+ T cells. While CD1c is thought to specifically identify this subset of human cDCs, we show here that also classical and intermediate monocytes express CD1c. Accordingly, the commercial CD1c (BDCA-1)+ Dendritic Cell Isolation Kit isolates two distinct cell populations from blood: CD1c+CD14- cDCs and CD1c+CD14+ monocytes. CD1c+ cDCs and CD1c+ monocytes exhibited strikingly different properties, including their differential regulation of surface marker expression, their levels of cytokine production, and their ability to stimulate naïve CD4+ T cells. These results demonstrate that a commercial CD1c (BDCA-1)+ Dendritic Cell Isolation Kit isolates two functionally different cell populations, which has important implications for the interpretation of previously generated data using this kit to characterize CD1c+ cDCs.
Department of Pathology, Oslo University Hospital, Oslo, Norway.|Centre for Immune Regulation, University of Oslo, Oslo, Norway.
Martine
M
Schrøder
Department of Pathology, Oslo University Hospital, Oslo, Norway.
Guro Reinholt
GR
Melum
Department of Pathology, Oslo University Hospital, Oslo, Norway.|Centre for Immune Regulation, University of Oslo, Oslo, Norway.
Ole J B
OJ
Landsverk
Department of Pathology, Oslo University Hospital, Oslo, Norway.|Centre for Immune Regulation, University of Oslo, Oslo, Norway.
Anna
A
Bujko
Department of Gastrointestinal Surgery, Oslo University Hospital, Oslo, Norway.
Sheraz
S
Yaqub
Department of Otolaryngology, Lovisenberg Diakonale Hospital, Oslo, Norway.
Einar
E
Gran
Department of Cardiothoracic Surgery, Oslo University Hospital, Oslo, Norway.|Tumor Immunology Group, Department of Pathology, Oslo University Hospital, Oslo, Norway.
Henrik
H
Aamodt
Department of Pathology, Oslo University Hospital, Oslo, Norway.|Centre for Immune Regulation, University of Oslo, Oslo, Norway.
Espen S
ES
Bækkevold
Department of Pathology, Oslo University Hospital, Oslo, Norway.|Centre for Immune Regulation, University of Oslo, Oslo, Norway.
Frode L
FL
Jahnsen
Department of Pathology, Oslo University Hospital, Oslo, Norway.|Centre for Immune Regulation, University of Oslo, Oslo, Norway.
Lisa
http://orcid.org/0000-0002-0769-619X
L
Richter
D018949:Antigens, CD1 / Q000235:genetics / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002450:Cell Communication; D002469:Cell Separation / Q000379:methods*; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D005434:Flow Cytometry / Q000592:standards*; D015870:Gene Expression; D006023:Glycoproteins / Q000235:genetics / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation; D009000:Monocytes / Q000166:cytology / Q000276:immunology*; D061251:Primary Cell Culture; D011933:Reagent Kits, Diagnostic
D018949:Antigens, CD1; C437944:CD1C protein, human; D006023:Glycoproteins; D011933:Reagent Kits, Diagnostic