Monitoring tetracycline through a solid-state nanopore sensor.
Antibiotics as emerging environmental contaminants, are widely used in both human and veterinary medicines. A solid-state nanopore sensing method is reported in this article to detect Tetracycline, which is based on Tet-off and Tet-on systems. rtTA (reverse tetracycline-controlled trans-activator) and TRE (Tetracycline Responsive Element) could bind each other under the action of Tetracycline to form one complex. When the complex passes through nanopores with 8 ~ 9 nanometers in diameter, we could detect the concentrations of Tet from 2 ng/mL to 2000 ng/mL. According to the Logistic model, we could define three growth zones of Tetracycline for rtTA and TRE. The slow growth zone is 0-39.5 ng/mL. The rapid growth zone is 39.5-529.7 ng/mL. The saturated zone is > 529.7 ng/mL. Compared to the previous methods, the nanopore sensor could detect and quantify these different kinds of molecule at the single-molecule level.
School of Physical Electronic, University of Electronic Science and Technology of China, Chengdu, P.R. China.|Chongqing Key Lab of Multi-scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, P.R. China.
Yuechuan
Y
Zhang
Research Center for Environment and Health, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, P.R. China.
Yanling
Y
Chen
School of Physical Electronic, University of Electronic Science and Technology of China, Chengdu, P.R. China.
Yongqi
Y
Fu
Chongqing Key Lab of Multi-scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, P.R. China.|Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, School of Physics, Nankai University, Tianjin, China.
Cuifeng
C
Ying
Chongqing Key Lab of Multi-scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, P.R. China.
Yanxiao
Y
Feng
Chongqing Key Lab of Multi-scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, P.R. China.
Qimeng
Q
Huang
Research Center for Environment and Health, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, P.R. China.
Chao
C
Wang
Research Center for Environment and Health, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, P.R. China.
De-Sheng
DS
Pei
Chongqing Key Lab of Multi-scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, P.R. China.
Deqiang
D
Wang
D000900:Anti-Bacterial Agents / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods*; D004785:Environmental Pollutants / Q000032:analysis; D058608:Nanopores; D013752:Tetracycline / Q000032:analysis*
D000900:Anti-Bacterial Agents; D004785:Environmental Pollutants; D013752:Tetracycline