A HClO-specific near-infrared fluorescent probe for determination of Myeloperoxidase activity and imaging mitochondrial HClO in living cells.
Hyperchlorous acid (HClO), produced from MPO, is recognized as a host defense that kills pathogens; a signaling molecule that initiates cell apoptosis; and a harmful agent when overproduced. Thus, measuring of endogenous HClO and MPO will always find its great importance in revealing biological roles under complex biological conditions. In this study, a turn-on near infrared (NIR) fluorescent probe Cy7-NphS has been designed and developed for highly selective and sensitive sensing of HClO and Myeloperoxidase (MPO) with fast response time. The newly developed probe has been successfully applied in real-time monitoring of HClO and MPO activity in PBS solutions and living HL-60 cells. When applied in MPO activity determination, the probe showed very high sensitivity with a detection limit of as low as 3.69×10(-3)U/mL. Furthermore, the living cell imaging study suggested that this probe could detect HClO in mitochondria.
Department of Chemistry, Zhoukou Normal University, Zhoukou 466001, PR China.
Fengshou
F
Tian
State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics (DICP), Chinese Academy of Sciences (CAS), 457 Zhongshan Road, Dalian 116023, PR China; Graduate School of the Chinese Academy of Sciences, Beijing 100039, PR China.
Yan
Y
Jia
Department of Chemistry, Zhoukou Normal University, Zhoukou 466001, PR China; State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics (DICP), Chinese Academy of Sciences (CAS), 457 Zhongshan Road, Dalian 116023, PR China; Graduate School of the Chinese Academy of Sciences, Beijing 100039, PR China.
Yanan
Y
Zhang
The First Affiliated Hospital of Dalian Medical University, 222 Zhongshan Road, 116011, PR China.
Wei
W
Song
State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics (DICP), Chinese Academy of Sciences (CAS), 457 Zhongshan Road, Dalian 116023, PR China; Graduate School of the Chinese Academy of Sciences, Beijing 100039, PR China.
Guangjiu
G
Zhao
State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics (DICP), Chinese Academy of Sciences (CAS), 457 Zhongshan Road, Dalian 116023, PR China; Graduate School of the Chinese Academy of Sciences, Beijing 100039, PR China.
Zongjin
Z
Qu
Department of Chemistry, Zhoukou Normal University, Zhoukou 466001, PR China; State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics (DICP), Chinese Academy of Sciences (CAS), 457 Zhongshan Road, Dalian 116023, PR China; Graduate School of the Chinese Academy of Sciences, Beijing 100039, PR China.
Chunyan
C
Li
Department of Chemistry, Zhoukou Normal University, Zhoukou 466001, PR China.
Yahong
Y
Chen
State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics (DICP), Chinese Academy of Sciences (CAS), 457 Zhongshan Road, Dalian 116023, PR China; Graduate School of the Chinese Academy of Sciences, Beijing 100039, PR China. Electronic address: pengl@dicp.ac.cn.
Peng
P
Li
D002232:Carbocyanines / Q000737:chemistry*; D004789:Enzyme Activation; D005456:Fluorescent Dyes / Q000737:chemistry*; D018922:HL-60 Cells; D006801:Humans; D006997:Hypochlorous Acid / Q000378:metabolism*; D007259:Infrared Rays; D008856:Microscopy, Fluorescence / Q000379:methods*; D008928:Mitochondria / Q000201:enzymology* / Q000648:ultrastructure; D057054:Molecular Imaging / Q000379:methods; D009195:Peroxidase / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D002232:Carbocyanines; D005456:Fluorescent Dyes; C501744:indotricarbocyanine; D006997:Hypochlorous Acid; D009195:Peroxidase
Bio-imaging; ClO(−)/MPO detection; Mitochondria targeting; Near-infrared; Real-time monitoring