Age Impaired endothelium-dependent vasodilation is improved by resveratrol in rat mesenteric arteries.
OBJECTIVE
To determine whether resveratrol improves the adverse effects age on vascular function in mesenteric arteries (MAs), and diminishes the hyperactivity in adrenal gland with age.


METHODS
Male F344 x Brown Norway rats were assigned to 6-month control (YC), 6-month resveratrol (YR), 24-month control (OC) and 24-month resveratrol (OR). Resveratrol (15 mg/kg) was provided to resveratrol groups in drinking water for 14 days.


RESULTS
Concentration response curves to phenylephrine (PE, 10(-9)-10(-5)M), acetylcholine (Ach, 10(-9)-10(-5)M) and resveratrol (10(-8)-10(-4)M) were evaluated in pressurized isolated MAs. The Ach concentration-response curve was right shifted with maximal response diminished in OC compared with YC rats. These effects were reversed by resveratrol treatment. The resveratrol-mediated relaxant responses were unchanged with age or resveratrol suggesting an endothelium-independent mechanism. Resveratrol tended to increase endothelial nitric oxide synthase; caused no effect on copper-zinc superoxide dismutase; and normalized the age-related elevatation in DβH and NPY levels in adrenal medulla, two indicators of sympathetic activity.


CONCLUSIONS
These data indicate that resveratrol reverses age-related dysfunction in endothelium-dependent vasodilation in MAs and partially reverses hyperactivity of adrenomedullary function with age. This treatment may have a therapeuticpotential in the treatment of cardiovascular diseases or hypertension in the elderly.
Department of Pharmacology, Kocaeli University, Kocaeli, 41380 Turkey.
Semil S
SS
Gocmez
Department of Pharmacology and Therapeutics, College of Medicine, University of Florida, Gainesville, FL 32610 United States.
Philip J
PJ
Scarpace
Department of Kinesiology, West Chester University, West Chester, PA 19383 United States.
Melissa A
MA
Whidden
Department of Pharmacology, University of Vermont, Burlington, VT 05405 United States.
Benedek
B
Erdos
Geriatric Research, Education, and Clinical Center, North Florida/ South Georgia Veterans Health System, Gainesville, FL 32608United States; Department of Pharmacology and Therapeutics, College of Medicine, University of Florida, Gainesville, FL 32610United States.
Nataliya
N
Kirichenko
Geriatric Research, Education, and Clinical Center, North Florida/ South Georgia Veterans Health System, Gainesville, FL 32608United States; Department of Pharmacology and Therapeutics, College of Medicine, University of Florida, Gainesville, FL 32610United States.
Yasemin
Y
Sakarya
Department of Pharmacology, Kocaeli University, Kocaeli, 41380 Turkey.
Tijen
T
Utkan
Geriatric Research, Education, and Clinical Center, North Florida/ South Georgia Veterans Health System, Gainesville, FL 32608United States; Department of Pharmacology and Therapeutics, College of Medicine, University of Florida, Gainesville, FL 32610United States.
Nihal
N
Tumer
Aging; adrenal; catecholamines; mesenteric artery; resveratrol