MiR-142-5p and miR-486-5p as biomarkers for early detection of chronic antibody-mediated rejection in kidney transplantation.
De novo donor-specific HLA antibody (DSA) would not necessarily contribute to chronic antibody-mediated rejection (CAMR) in kidney transplantation. Here, we investigated whether PBMC miRNAs could be predictable biomarkers for CAMR. Microarray profiling of 435 mature miRNAs in pooled samples was conducted. Individual analysis revealed that miR-142-5p was significantly (p < 0.01) underexpressed in patients with DSA. After DSA production, miR-486-5p and its target PTEN/foxO3 mRNA were significantly overexpressed (p < 0.01) and underexpressed (p < 0.01), respectively, in patients with biopsy-proven CAMR, compared with non-CAMR. Our studies suggest that miRNA expression patterns may serve as noninvasive diagnostic biomarkers to evaluate immune response and kidney allograft status.
a Department of Kidney Disease and Transplant Immunology , Aichi Medical University , Nagakute , Japan.
Kenta
K
Iwasaki
b Department of Transplant Surgery , Nagoya Daini Red Cross Hospital , Nagoya , Japan.
Takayuki
T
Yamamoto
c Department of Surgery II , Nagoya University School of Medicine , Nagoya , Japan.
Yukiko
Y
Inanaga
b Department of Transplant Surgery , Nagoya Daini Red Cross Hospital , Nagoya , Japan.
Takahisa
T
Hiramitsu
c Department of Surgery II , Nagoya University School of Medicine , Nagoya , Japan.
Yuko
Y
Miwa
d Department of Center for Clinical Research , Aichi Medical University , Nagakute , Japan.
Kenta
K
Murotani
b Department of Transplant Surgery , Nagoya Daini Red Cross Hospital , Nagoya , Japan.
Shuji
S
Narumi
b Department of Transplant Surgery , Nagoya Daini Red Cross Hospital , Nagoya , Japan.
Yoshihiko
Y
Watarai
e Department of Transplant Surgery , Masuko Memorial Hospital , Nagoya , Japan.
Akio
A
Katayama
a Department of Kidney Disease and Transplant Immunology , Aichi Medical University , Nagakute , Japan.|f Department of Transplant Surgery , Aichi Medical University , Nagakute , Japan.
Kazuharu
K
Uchida
f Department of Transplant Surgery , Aichi Medical University , Nagakute , Japan.
Takaaki
T
Kobayashi
D000906:Antibodies / Q000009:adverse effects; D015415:Biomarkers / Q000097:blood; D002908:Chronic Disease; D042241:Early Diagnosis; D006084:Graft Rejection / Q000235:genetics* / Q000276:immunology; D006801:Humans; D016030:Kidney Transplantation; D035683:MicroRNAs / Q000097:blood*
D000906:Antibodies; D015415:Biomarkers; C555159:MIRN142 microRNA, human; C560945:MIRN486 microRNA, human; D035683:MicroRNAs
Chronic antibody-mediated rejection; diagnosis; kidney transplantation; microRNA