Pilot-scale bubble column photobioreactor culture of a marine dinoflagellate microalga illuminated with light emission diodes.
Production of biomass of the shear-sensitive marine algal dinoflagellate Karlodinium veneficum was successfully scaled-up to 80L using a bubble column photobioreactor. The scale factor exceeded 28,500. Light-emission diodes were used as the light source. The diel irradiance profile mimicked the outdoor profile of natural sunlight. The final cell concentration in the absence of nutrient limitation in the scaled-up photobioreactor was nearly 12×10(5)cellsmL(-1), or the same as in laboratory culture systems. The pH-controlled culture (pH=8.5) was always carbon-sufficient. The culture was mixed pneumatically by using a superficial air velocity of 1.9×10(-3)ms(-1) and the temperature was controlled at 21±1°C.
Chemical Engineering Area, University of Almería, 04120 Almería, Spain.
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López-Rosales
Chemical Engineering Area, University of Almería, 04120 Almería, Spain. Electronic address: fgarcia@ual.es.
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García-Camacho
Chemical Engineering Area, University of Almería, 04120 Almería, Spain.
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Sánchez-Mirón
Chemical Engineering Area, University of Almería, 04120 Almería, Spain.
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Martín Beato
School of Engineering, Massey University, Private Bag 11 222, Palmerston North, New Zealand.
Yusuf
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Chisti
Chemical Engineering Area, University of Almería, 04120 Almería, Spain.
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Molina Grima
D018533:Biomass; D004141:Dinoflagellida / Q000254:growth & development*; D008027:Light; D058433:Photobioreactors
Dinoflagellates; Irradiance model; Karlodinium veneficum; LED; Microalgae; Photobioreactor