Isolation and characterization of Neisseria musculi sp. nov., from the wild house mouse.
Members of the genus Neisseria have been isolated from or detected in a wide range of animals, from non-human primates and felids to a rodent, the guinea pig. By means of selective culture, biochemical testing, Gram staining and PCR screening for the Neisseria-specific internal transcribed spacer region of the rRNA operon, we isolated four strains of the genus Neisseria from the oral cavity of the wild house mouse, Mus musculus subsp. domesticus. The isolates are highly related and form a separate clade in the genus, as judged by tree analyses using either multi-locus sequence typing of ribosomal genes or core genes. One isolate, provisionally named Neisseria musculi sp. nov. (type strain AP2031T=DSM 101846T=CCUG 68283T=LMG 29261T), was studied further. Strain AP2031T/N. musculi grew well in vitro. It was naturally competent, taking up DNA in a DNA uptake sequence and pilT-dependent manner, and was amenable to genetic manipulation. These and other genomic attributes of N. musculi sp. nov. make it an ideal candidate for use in developing a mouse model for studying Neisseria-host interactions.
Department of Immunobiology and BIO5 Institute, University of Arizona, Tucson, AZ 85719, USA.
Nathan J
NJ
Weyand
Department of Immunobiology and BIO5 Institute, University of Arizona, Tucson, AZ 85719, USA.
Mancheong
M
Ma
Department of Ecology and Evolutionary Biology, University of Arizona, Tucson, AZ 85721, USA.
Megan
M
Phifer-Rixey
Department of Immunobiology and BIO5 Institute, University of Arizona, Tucson, AZ 85719, USA.
Nyiawung A
NA
Taku
Department of Immunobiology and BIO5 Institute, University of Arizona, Tucson, AZ 85719, USA.
María A
MA
Rendón
Department of Immunobiology and BIO5 Institute, University of Arizona, Tucson, AZ 85719, USA.
Alyson M
AM
Hockenberry
Department of Immunobiology and BIO5 Institute, University of Arizona, Tucson, AZ 85719, USA.
Won J
WJ
Kim
School of Animal and Comparative Biomedical Sciences, University of Arizona, Tucson, AZ 85721, USA.
Al B
AB
Agellon
Department of Biology, Brooklyn College of the City University of New York, Brooklyn, NY 11210, USA.
Nicolas
N
Biais
Department of Ecology and Evolutionary Biology, University of Arizona, Tucson, AZ 85721, USA.
Taichi A
TA
Suzuki
Department of Zoology, University of Oxford, Oxford OX1 3PS, UK.
Lily
L
Goodyer-Sait
Department of Zoology, University of Oxford, Oxford OX1 3PS, UK.
Odile B
OB
Harrison
Department of Zoology, University of Oxford, Oxford OX1 3PS, UK.
Holly B
HB
Bratcher
Department of Ecology and Evolutionary Biology, University of Arizona, Tucson, AZ 85721, USA.
Michael W
MW
Nachman
Department of Zoology, University of Oxford, Oxford OX1 3PS, UK.
Martin C J
MCJ
Maiden
Department of Immunobiology and BIO5 Institute, University of Arizona, Tucson, AZ 85719, USA.
Magdalene
M
So
D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D051379:Mice / Q000382:microbiology*; D009055:Mouth / Q000382:microbiology; D058885:Multilocus Sequence Typing; D009343:Neisseria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009656:North America; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S