Extraction and Analysis of Peptidoglycan Cell Wall Precursors.
Extraction and analysis by LC-MS of peptidoglycan precursors represent a valuable method to study antibiotic mode of action and resistance in bacteria. Here, we describe how to apply this method for: (1) testing the action of different classes of antibiotics inhibiting cell wall biosynthesis in Bacillus megaterium; (2) studying the mechanism of self-resistance in mycelial actinomycetes producing glycopeptide antibiotics.
Department of Biotechnology and Life Sciences, University of Insubria, Via Dunant, Varese, 3-21100, Italy.|"The Protein Factory" Research Center, Politecnico of Milano, ICRM CNR Milano University of Insubria, Via Dunant, Varese, 3-21100, Italy.
Elisa
E
Binda
Fondazione Istituto Insubrico Ricerca per la Vita (F.I.I.R.V.), Via R. Lepetit 32, Gerenzano, 21100, Italy.
Lùcia
L
Carrano
Department of Biotechnology and Life Sciences, University of Insubria, Via Dunant, Varese, 3-21100, Italy.|"The Protein Factory" Research Center, Politecnico of Milano, ICRM CNR Milano University of Insubria, Via Dunant, Varese, 3-21100, Italy.
Giorgia Letizia
GL
Marcone
Department of Biotechnology and Life Sciences, University of Insubria, Via Dunant, Varese, 3-21100, Italy. flavia.marinelli@uninsubria.it.|"The Protein Factory" Research Center, Politecnico of Milano, ICRM CNR Milano University of Insubria, Via Dunant, Varese, 3-21100, Italy. flavia.marinelli@uninsubria.it.
Flavia
F
Marinelli
D000192:Actinomycetales / Q000254:growth & development / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001410:Bacillus megaterium / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism*; D002853:Chromatography, Liquid; D024881:Drug Resistance, Bacterial; D006020:Glycopeptides / Q000032:analysis* / Q000302:isolation & purification* / Q000494:pharmacology; D010457:Peptidoglycan / Q000096:biosynthesis*; D053719:Tandem Mass Spectrometry
D000900:Anti-Bacterial Agents; D006020:Glycopeptides; D010457:Peptidoglycan
Actinoplanes teichomyceticus; Antibiotics; Bacillus megaterium; Bacitracin; Glycopeptides; LC-MS; Lantibiotics; Nonomuraea sp. ATCC 39727; Peptidoglycan precursors; Ramoplanin