Enterovirus but not Parvovirus B19 is associated with idiopathic dilated cardiomyopathy and endomyocardial CD3, CD68, or HLA-DR expression.
We assessed Enterovirus (EV) &Parvovirus B19 (PVB19) genomes and CD3, CD68&HLA-DR detection in dilated cardiomyopathies (DCM). EV&PVB19 genomes and CD3, CD68&HLA-DR were detected by PCR and immunohistochemistry assays in 115 endomyocardial biopsies obtained in 13 idiopathic DCM (iDCM) and 10 explained DCM (eDCM) patients. Results were compared with those of 47 atrial surgical samples (47 surgery controls) and 22 autoptic cardiac samples (11 healthy heart controls) (2008-2014, Reims, France). EV was detected in 23.1% of iDCM patients but not in eDCM and controls (P = 0.003) (viral load 803 copies/μg). PVB19 was detected in 76.9%, 80.0%, 63.6% and 78.2% of iDCM, eDCM, healthy heart and surgery controls (P = 0.99) with a mean viral load of 413, 346, 1,428, and 71 copies/μg. CD3, CD68 or HLA-DR were detected in 100 and 50% of EV and PVB19 "mono-infected" iDCM patients. EV was exclusively detected in iDCM cases in association with CD3, CD68, or HLA-DR indicating that EV could be an etiological cause in a subset of iDCM cases. By contrast the equal frequent detection of PVB19 in iDCM cases and controls without association with CD3, CD68, or HLA-DR suggested that PVB19 could be a bystander in many DCM cases. J. Med. Virol. 89:55-63, 2017. © 2016 Wiley Periodicals, Inc.
Laboratoirede Virologie médicale et moléculaire, Centre hospitalier universitaire de Reims, France. yohan.nguyen@wanadoo.fr.|EA-4684 Cardiovir, Faculté de médecine, Université de Reims Champagne-Ardenne, Reims, France. yohan.nguyen@wanadoo.fr.
Yohan
Y
N'Guyen
EA-4684 Cardiovir, Faculté de médecine, Université de Reims Champagne-Ardenne, Reims, France.|Service de Cardiologie, Centre hospitalier universitaire de Reims, France.
François
F
Lesaffre
Service de Cardiologie, Centre hospitalier universitaire de Reims, France.
Damien
D
Metz
Service de Cardiologie, Centre hospitalier universitaire de Reims, France.
Sophie
S
Tassan
Service de Chirurgie Thoracique, Centre hospitalier universitaire de Reims, France.
Yves
Y
Saade
Laboratoire d'Anatomie Pathologique, Faculté de médecine, Université de Reims Champagne Ardennes, Reims, France.|Laboratoire d'Anatomie Pathologique, Centre hospitalier universitaire de Reims, France.
Camille
C
Boulagnon
Laboratoire d'Anatomie Pathologique, Faculté de médecine, Université de Reims Champagne Ardennes, Reims, France.|Laboratoire d'Anatomie Pathologique, Centre hospitalier universitaire de Reims, France.
Paul
P
Fornes
Laboratoirede Virologie médicale et moléculaire, Centre hospitalier universitaire de Reims, France.|EA-4684 Cardiovir, Faculté de médecine, Université de Reims Champagne-Ardenne, Reims, France.
Fanny
F
Renois
Laboratoirede Virologie médicale et moléculaire, Centre hospitalier universitaire de Reims, France.|EA-4684 Cardiovir, Faculté de médecine, Université de Reims Champagne-Ardenne, Reims, France.
Laurent
L
Andreoletti
D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000096:biosynthesis*; D015214:Antigens, Differentiation, Myelomonocytic / Q000096:biosynthesis*; D017252:CD3 Complex / Q000096:biosynthesis*; D002311:Cardiomyopathy, Dilated / Q000473:pathology* / Q000821:virology*; D004699:Endocardium / Q000473:pathology; D004770:Enterovirus / Q000302:isolation & purification*; D005260:Female; D005602:France; D006684:HLA-DR Antigens / Q000096:biosynthesis*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009206:Myocardium / Q000473:pathology; D016732:Parvovirus B19, Human / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D011446:Prospective Studies; D000097382:CD68 Molecule
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D017252:CD3 Complex; D006684:HLA-DR Antigens; C067980:CD68 antigen, human; D000097382:CD68 Molecule
dilated cardiomyopathy; enterovirus; inflammatory markers; parvovirus B19