Protective Effect of Polydeoxyribonucleotide Against Renal Ischemia-Reperfusion Injury in Mice.
BACKGROUND
Polydeoxyribonucleotide (PDRN) is an A2A receptor agonist that induces vascular endothelial growth factor (VEGF) production during the pathological condition of low tissue perfusion. Ischemia-reperfusion injury (IRI) is a major problem after renal transplantation. In the present study, we investigated whether PDRN exhibits reno-protective effects against ischemia-reperfusion-induced acute kidney injury in mice.


METHODS
Renal ischemia-reperfusion injury was induced in male C57BL/6 mice by bilateral renal pedicle occlusion for 30 minutes, followed by reperfusion for 48 hours. PDRN (8 mg/kg body weight intraperitoneally) was administered 30 minutes before IRI.


RESULTS
Treatment with PDRN significantly decreased neutrophil gelatinase-associated lipocalin levels in the urine, blood urea nitrogen level, and serum creatinine levels as well as kidney tubular injury. Western blotting showed that PDRN significantly increased the levels of vascular endothelial growth factor and B-cell lymphoma protein and attenuated p38 mitogen-activated protein kinase, c-Jun N-terminal kinase, inducible nitric oxide synthase, and Bcl-2-associated X protein levels 48 hours after IRI.


CONCLUSIONS
Our findings suggest that PDRN is a potential therapeutic agent for acute ischemia-induced renal damage.
Department of Anesthesia and Pain Medicine, Ulsan University College of Medicine, Gangneung Asan Hospital, Gangneung, South Korea.
E K
EK
Jeong
Department of Surgery, Ulsan University College of Medicine, Gangneung Asan Hospital, Gangneung, South Korea. Electronic address: jhj@gnah.co.kr.
H J
HJ
Jang
Department of Anesthesia and Pain Medicine, Ulsan University College of Medicine, Gangneung Asan Hospital, Gangneung, South Korea.
S S
SS
Kim
Department of Surgery, Ulsan University College of Medicine, Gangneung Asan Hospital, Gangneung, South Korea.
S Y
SY
Lee
Department of Surgery, Ulsan University College of Medicine, Gangneung Asan Hospital, Gangneung, South Korea.
M Y
MY
Oh
Department of Surgery, Ulsan University College of Medicine, Gangneung Asan Hospital, Gangneung, South Korea.
H J
HJ
Kim
Department of Pathology, Ulsan University College of Medicine, Gangneung Asan Hospital, Gangneung, South Korea.
D W
DW
Eom
Natural Medicine Center, Korea Institute of Science and Technology (KIST), Gangneung, South Korea.
J Y
JY
Ham
Asan Medical Center, Seoul, South Korea.
D J
DJ
Han
D058186:Acute Kidney Injury / Q000175:diagnosis / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D016030:Kidney Transplantation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011089:Polydeoxyribonucleotides / Q000627:therapeutic use*; D020011:Protective Agents / Q000627:therapeutic use*; D011897:Random Allocation; D015427:Reperfusion Injury / Q000175:diagnosis / Q000378:metabolism / Q000517:prevention & control*; D016896:Treatment Outcome
D015415:Biomarkers; D011089:Polydeoxyribonucleotides; D020011:Protective Agents