Iron deficiency cause changes in photochemistry, thylakoid organization, and accumulation of photosystem II proteins in Chlamydomonas reinhardtii.
A trace element, iron (Fe) plays a pivotal role in photosynthesis process which in turn mediates the plant growth and productivity. Here, we have focused majorly on the photochemistry of photosystem (PS) II, abundance of proteins, and organization of supercomplexes of thylakoids from Fe-depleted cells in Chlamydomonas reinhardtii. Confocal pictures show that the cell's size has been reduced and formed rosette-shaped palmelloids; however, there is no cell death. Further, the PSII photochemistry was reduced remarkably. Further, the photosynthetic efficiency analyzer data revealed that both donor and acceptor side of PSII were equally damaged. Additionally, the room-temperature emission spectra showed the fluorescence emission maxima increased due to impaired energy transfer from PSII to PSI. Furthermore, the protein data reveal that most of the proteins of reaction center and light-harvesting antenna were reduced in Fe-depleted cells. Additionally, the supercomplexes of PSI and PSII were destabilized from thylakoids under Fe-deficient condition showing that Fe is an important element in photosynthesis mechanism.
Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, 500046, India.
Elsin Raju
ER
Devadasu
Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, 500046, India.
Sai Kiran
SK
Madireddi
Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, 500046, India.
Srilatha
S
Nama
Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, 500046, India. srgsl@uohyd.ernet.in.
Rajagopal
R
Subramanyam
D016825:Chlamydomonas reinhardtii / Q000378:metabolism / Q000502:physiology*; D002734:Chlorophyll / Q000378:metabolism / Q000502:physiology; D000077194:Chlorophyll A; D005453:Fluorescence; D007501:Iron / Q000502:physiology; D000090463:Iron Deficiencies; D018613:Microscopy, Confocal; D055668:Photochemical Processes; D045332:Photosystem II Protein Complex / Q000378:metabolism / Q000502:physiology*; D020524:Thylakoids / Q000378:metabolism / Q000502:physiology*
D045332:Photosystem II Protein Complex; D002734:Chlorophyll; D007501:Iron; D000077194:Chlorophyll A
Blue native gel electrophoresis; Fe deficiency; Light-harvesting complexes; Photochemistry; Photosystem II; Thylakoid organization