The Physiological and Molecular Characterization of a Small Colony Variant of Escherichia coli and Its Phenotypic Rescue.
Small colony variants (SCVs) can be defined as a naturally occurring sub-population of bacteria characterized by their reduced colony size and distinct biochemical properties. SCVs of Staphylococcus aureus have been studied extensively over the past two decades due to their role in recurrent human infections. However, little work has been done on SCVs of Escherichia coli, and this work has focused on the physiology and morphology that define these colonies of E. coli, such as small size and slow growth. E. coli strain JW0623, has a null lipA mutation in the lipoic acid synthase gene (lipA), and is a lipoic acid auxotroph. When the mutant was grown in LB medium to log phase, it showed remarkable resistance to acid (pH 3), hydrogen peroxide, heat and osmotic stress compared to its parent BW25113. Using RT-PCR and real time RT-PCR, the expression of certain genes was compared in the two strains in an attempt to create a molecular profile of Escherichia coli SCVs. These include genes involved in glycolysis, TCA cycle, electron transport, iron acquisition, biofilm formation and cyclopropane fatty acid synthesis. It was also demonstrated that the addition of 5 μg/ml of lipoic acid to LB medium allows for the phenotypic rescue of the mutant; reversing its slow growth, its resistance characteristics, and elevated gene expression. These results indicate that the mutation in lipA leads to an E. coli SCV that resembles an electron transport defective SCV of S. aureus These strains are typically auxotrophs, and are phenotypically rescued by adding the missing metabolite to rich medium. There are global shifts in gene expression which are reversible and depend on whether the auxotrophic molecule is absent or present. Looking at the E. coli SCV from an evolutionary point of view, it becomes evident that its path to survival is to express genes that confer stress resistance.
Department of Biological Sciences, St. John's University, Queens, New York, United States of America.
Victor
http://orcid.org/0000-0001-8228-0955
V
Santos
Department of Biological Sciences, St. John's University, Queens, New York, United States of America.
Irvin
I
Hirshfield
D000222:Adaptation, Physiological / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development; D002952:Citric Acid Cycle / Q000235:genetics; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology; D003521:Cyclopropanes / Q000378:metabolism; D004579:Electron Transport / Q000235:genetics; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D005227:Fatty Acids / Q000096:biosynthesis; D015964:Gene Expression Regulation, Bacterial; D006019:Glycolysis / Q000235:genetics; D006358:Hot Temperature; D006861:Hydrogen Peroxide / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000378:metabolism; D009154:Mutation; D009997:Osmotic Pressure; D010641:Phenotype; D013312:Stress, Physiological; D008063:Thioctic Acid / Q000172:deficiency* / Q000494:pharmacology
D001426:Bacterial Proteins; D003470:Culture Media; D003521:Cyclopropanes; D005227:Fatty Acids; C079999:LipA protein, Bacteria; D008063:Thioctic Acid; C030797:cyclopropane; D006861:Hydrogen Peroxide; D007501:Iron