Myxobolus ictiobus n. sp. and Myxobolus minutus n. sp. (Cnidaria: Myxobolidae) from the gills of the smallmouth buffalo Ictiobus bubalus Rafinesque (Cypriniformes: Catostomidae).
The smallmouth buffalo Ictiobus bubalus Rafinesque (Catostomidae) is native to North American waterways and occasionally grown in pond aquaculture. Species of Myxobolus Bütschli, 1882 have been reported from the gills, integument, and intestinal tract of buffalo fish, although there is ambiguity in some host records. In the summer of 2013, thirteen adult smallmouth buffalo were seined from a 0.1-acre (0.04-hectare) experimental research pond at the Thad Cochran National Warmwater Aquaculture Center in Stoneville, Mississippi, USA, and examined for the presence of parasitic infection. Two previously unknown species of Myxobolus were observed parasitising the gills. Plasmodia of the two species differed from each other in both size and shape. Morphologically the two species were distinct from one another and from other Myxobolus spp. previously reported from buffalo fish. Myxospores of Myxobolus ictiobus n. sp. were spherical and measured 12.7-14.5 (13.9 ± 0.4) µm in length and 10.7-13.6 (12.5 ± 0.7) µm in width with a thickness of 10.3-14.8 (12.6 ± 2.3) µm. Polar capsules measured 5.6-7.4 (6.6 ± 0.4) µm in length and 3.7-4.9 (4.5 ± 0.8) µm in width and each contained a coiled polar filament with 5-6 turns. Myxospores of Myxobolus minutus n. sp. were circular in shape and measured 7.4-9.6 (8.6 ± 0.7) µm in length and 7.5-9.9 (8.8 ± 0.7) µm in width with a thickness of 6.5-7.3 (6.7 ± 0.3) µm. Polar capsules measured 3.6-4.9 (4.3 ± 0.3) µm in length and 2.8-3.8 (3.3 ± 0.3) µm and each contained a coiled polar filament with 5-6 turns. Supplemental 18S rRNA gene sequencing identified unique sequences for each isolate. Phylogenetic analysis of 18S rRNA sequences demonstrated a strong clustering of both isolates with other species of Myxobolus from cypriniform fish.
Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS, 39762, USA.
Thomas G
TG
Rosser
Department of Pathobiology and Population Medicine, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS, 39762, USA. matt.griffin@msstate.edu.|Thad Cochran National Warmwater Aquaculture Center, Aquatic Research and Diagnostic Laboratory, Delta Research and Extension Center, Mississippi State University, Stoneville, MS, 38776, USA. matt.griffin@msstate.edu.
Matt J
MJ
Griffin
Warmwater Aquaculture Research Unit, Agricultural Research Service, United States Department of Agriculture, Stoneville, MS, 38776, USA.
Sylvie M A
SM
Quiniou
Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS, 39762, USA.
Neely R
NR
Alberson
Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS, 39762, USA.
Ethan T
ET
Woodyard
Thad Cochran National Warmwater Aquaculture Center, Aquatic Research and Diagnostic Laboratory, Delta Research and Extension Center, Mississippi State University, Stoneville, MS, 38776, USA.|Mississippi Agricultural and Forestry Experiment Station, Mississippi State University, Mississippi State, MS, 39762, USA.
Charles C
CC
Mischke
Thad Cochran National Warmwater Aquaculture Center, Aquatic Research and Diagnostic Laboratory, Delta Research and Extension Center, Mississippi State University, Stoneville, MS, 38776, USA.|Mississippi Agricultural and Forestry Experiment Station, Mississippi State University, Mississippi State, MS, 39762, USA.
Terrence E
TE
Greenway
Thad Cochran National Warmwater Aquaculture Center, Aquatic Research and Diagnostic Laboratory, Delta Research and Extension Center, Mississippi State University, Stoneville, MS, 38776, USA.|Mississippi Agricultural and Forestry Experiment Station, Mississippi State University, Mississippi State, MS, 39762, USA.
David J
DJ
Wise
Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS, 39762, USA.
Linda M
LM
Pote
D000818:Animals; D003531:Cypriniformes / Q000469:parasitology*; D005880:Gills / Q000469:parasitology; D008922:Mississippi; D055766:Myxobolus / Q000145:classification* / Q000166:cytology / Q000235:genetics; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D013045:Species Specificity
D012337:RNA, Ribosomal, 18S