Gene expression analysis at multiple time-points identifies key genes for nerve regeneration.
The purpose of this study was to provide a comprehensive understanding of gene expression during Wallerian degeneration and axon regeneration after peripheral nerve injury.



A microarray was used to detect gene expression in the distal nerve 0, 3, 7, and 14 days after sciatic nerve crush. Bioinformatic analysis was used to predict function of the differentially expressed mRNAs. Microarray results and the key pathways were validated by quantitative real-time polymerase chain reaction (qRT-PCR).



Differentially expressed mRNAs at different time-points (3, 7, and 14 days) after injury were identified and compared with a control group (0 day). Nine general trends of changes in gene expression were identified. Key signal pathways and 9 biological processes closely associated with nerve regeneration were identified and verified.



Differentially expressed genes and biological processes and pathways associated with axonal regeneration may elucidate the molecular-biological mechanisms underlying peripheral nerve regeneration. Muscle Nerve 55: 373-383, 2017.
Department of Orthopaedics, Tianjin Medical University General Hospital, No. 154 Anshan Road, Heping District, Tianjin, 300052, PR China.
Bin
B
Pan
Department of Orthopaedics, Tianjin Medical University General Hospital, No. 154 Anshan Road, Heping District, Tianjin, 300052, PR China.
Yi
Y
Liu
Department of Orthopaedics, Tianjin Medical University General Hospital, No. 154 Anshan Road, Heping District, Tianjin, 300052, PR China.
Jia-Yin
JY
Yan
Department of Orthopaedics, Tianjin Medical University General Hospital, No. 154 Anshan Road, Heping District, Tianjin, 300052, PR China.
Yao
Y
Wang
Department of Orthopaedics, Tianjin Medical University General Hospital, No. 154 Anshan Road, Heping District, Tianjin, 300052, PR China.
Xue
X
Yao
Department of Orthopaedics, Tianjin Medical University General Hospital, No. 154 Anshan Road, Heping District, Tianjin, 300052, PR China.
Heng-Xing
HX
Zhou
Department of Orthopaedics, Tianjin Medical University General Hospital, No. 154 Anshan Road, Heping District, Tianjin, 300052, PR China.
Lu
L
Lu
School of Medicine, Nankai University, Nankai District, Tianjin, PR China.
Xiao-Hong
XH
Kong
Department of Orthopaedics, Tianjin Medical University General Hospital, No. 154 Anshan Road, Heping District, Tianjin, 300052, PR China.
Shi-Qing
SQ
Feng
D000818:Animals; D019295:Computational Biology; D004195:Disease Models, Animal; D015870:Gene Expression / Q000502:physiology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D046228:Microarray Analysis; D009416:Nerve Regeneration / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D020426:Sciatic Neuropathy / Q000503:physiopathology*; D015398:Signal Transduction / Q000235:genetics*; D013997:Time Factors
D012333:RNA, Messenger
Schwann cell; Wallerian degeneration; mRNA; microarray; peripheral nerve injury; regeneration