Synthesis and characterization of cryogel structures for isolation of EPSs from Botryococcus braunii.
In this study, the objective was to separate exopolysaccharides (EPSs) released in the broth subsequent to outdoor cultivation of Botryococcus braunii. For this, poly(2-hydroxyethyl methacrylate) (PHEMA) cryogels were synthesized. After that, the surface was modified by coupling Concanavalin A. Box-Behnken statistical design was used to evaluate the effect of freezing temperature, Con A concentration and flow rate on Con A binding capacity. Optimum synthesis conditions were elicited as -14.48°C freezing temperature, 1.00mg/ml Con A concentration and 0.30ml/min flow rate yielding 3.18mg Con A/g cryogel, whereas -16°C, 1.00mg/ml and 0.30ml/min yielded the highest (3.38mg) binding capacity in experimental cryogel preparation. The EPS adsorption capacity of the optimum cryogel column was found as 3.26mg EPS/g cryogel corresponding to adsorption yield of 80%. Besides; swelling test, elemental analysis, Micro-CT, SEM and FTIR analysis were carried out for characterization of the synthesized cryogels.
Department of Materials Science and Engineering, Ege University, 35100, Izmir, Turkey.
Irem Cemre
IC
Turu
Department of Biochemistry, Ege University, 35100, Izmir, Turkey.
Ceren
C
Turkcan-Kayhan
Novel Fluidic Technologies Group, Department of Bioengineering, Ege University, 35100, Izmir, Turkey.
Aslihan
A
Kazan
Novel Fluidic Technologies Group, Department of Bioengineering, Ege University, 35100, Izmir, Turkey.
Ece
E
Yildiz-Ozturk
Department of Biochemistry, Ege University, 35100, Izmir, Turkey.
Sinan
S
Akgol
Novel Fluidic Technologies Group, Department of Bioengineering, Ege University, 35100, Izmir, Turkey. Electronic address: ozlem.yesil.celiktas@ege.edu.tr.
Ozlem
O
Yesil-Celiktas
D060326:Chemistry Techniques, Synthetic; D000460:Chlorophyta / Q000737:chemistry*; D003208:Concanavalin A / Q000737:chemistry; D059985:Cryogels / Q000138:chemical synthesis* / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D008689:Methacrylates / Q000737:chemistry; D058105:Polymerization; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*; D013499:Surface Properties; D013696:Temperature
D059985:Cryogels; D008689:Methacrylates; D011134:Polysaccharides; D003208:Concanavalin A; C005044:hydroxyethyl methacrylate
B. braunii; Cryogel; Exopolysaccharides; PHEMA; Synthesis