Chemically Engineered Nanoparticle-Protein Interface for Real-Time Cellular Oxidative Stress Monitoring.
A co-engineered nanoparticle/protein peroxide detector is created. This system features a gold nanoparticle functionalized with a galactose headgroup (AuNP-Gal) that reacts covalently with a boronate-modified green fluorescent protein (PB-GFP). Boronate acid-saccharide complexation between PB-GFP and AuNP-Gal affords a highly stable assembly. This complex is disrupted by peroxide, allowing quantitative and selective monitoring of hydrogen peroxide production in real time.
Department of Chemistry, University of Massachusetts Amherst, 710 North Pleasant Street, Amherst, MA, 01003, USA.
Ying
Y
Jiang
Department of Biomedical Engineering, Tufts University, 4 Colby Street, Medford, MA, 02115, USA.
Ming
M
Wang
Department of Chemistry, University of Massachusetts Amherst, 710 North Pleasant Street, Amherst, MA, 01003, USA.
Joseph
J
Hardie
Department of Chemistry, University of Massachusetts Amherst, 710 North Pleasant Street, Amherst, MA, 01003, USA.
Gulen Yesilbag
GY
Tonga
Department of Chemistry, University of Massachusetts Amherst, 710 North Pleasant Street, Amherst, MA, 01003, USA.
Moumita
M
Ray
Department of Biomedical Engineering, Tufts University, 4 Colby Street, Medford, MA, 02115, USA.
Qiaobing
Q
Xu
Department of Chemistry, University of Massachusetts Amherst, 710 North Pleasant Street, Amherst, MA, 01003, USA.
Vincent M
VM
Rotello
D015374:Biosensing Techniques / Q000379:methods*; D005690:Galactose / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D018384:Oxidative Stress / Q000502:physiology*
D049452:Green Fluorescent Proteins; D006046:Gold; D006861:Hydrogen Peroxide; D005690:Galactose
cellular oxidative stress; chemically modified protein; gold nanoparticles; green fluorescent proteins; hydrogen peroxide