Retinal Electrophysiological Effects of Intravitreal Bone Marrow Derived Mesenchymal Stem Cells in Streptozotocin Induced Diabetic Rats.
Diabetic retinopathy is the most common cause of legal blindness in developed countries at middle age adults. In this study diabetes was induced by streptozotocin (STZ) in male Wistar albino rats. After 3 months of diabetes, rights eye were injected intravitreally with green fluorescein protein (GFP) labelled bone marrow derived stem cells (BMSC) and left eyes with balanced salt solution (Sham). Animals were grouped as Baseline (n = 51), Diabetic (n = 45), Diabetic+BMSC (n = 45 eyes), Diabetic+Sham (n = 45 eyes), Healthy+BMSC (n = 6 eyes), Healthy+Sham (n = 6 eyes). Immunohistology analysis showed an increased retinal gliosis in the Diabetic group, compared to Baseline group, which was assessed with GFAP and vimentin expression. In the immunofluorescence analysis BMSC were observed to integrate mostly into the inner retina and expressing GFP. Diabetic group had prominently lower oscillatory potential wave amplitudes than the Baseline group. Three weeks after intravitreal injection Diabetic+BMSC group had significantly better amplitudes than the Diabetic+Sham group. Taken together intravitreal BMSC were thought to improve visual function.
Marmara University School of Medicine, Department of Ophthalmology, Istanbul, Turkey.
Eren
http://orcid.org/0000-0002-8681-9214
E
Çerman
Genetic Engineering and Biotechnology Institution, The Scientific and Technological Research Council of Turkey, Kocaeli, Turkey.
Tolga
T
Akkoç
Marmara University School of Medicine, Department of Ophthalmology, Istanbul, Turkey.
Muhsin
M
Eraslan
Marmara University School of Medicine, Department of Ophthalmology, Istanbul, Turkey.
Özlem
Ö
Şahin
Haydarpaşa Numune Education and Research Hospital, Department of Pathology, Istanbul, Turkey.
Selvinaz
S
Özkara
Haydarpaşa Numune Education and Research Hospital, Department of Pathology, Istanbul, Turkey.
Fugen
F
Vardar Aker
Kocaeli University Center for Stem Cell and Gene Therapies, Kocaeli, Turkey.
Cansu
C
Subaşı
Kocaeli University Center for Stem Cell and Gene Therapies, Kocaeli, Turkey.
Erdal
E
Karaöz
Marmara University School of Medicine, Department of Pediatric Allergy and Immunology, Istanbul, Turkey.
Tunç
T
Akkoç
D000818:Animals; D002478:Cells, Cultured; D003921:Diabetes Mellitus, Experimental / Q000150:complications*; D003930:Diabetic Retinopathy / Q000473:pathology / Q000503:physiopathology* / Q000628:therapy*; D058449:Intravitreal Injections; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D017208:Rats, Wistar; D012160:Retina / Q000166:cytology / Q000473:pathology / Q000503:physiopathology*; D013311:Streptozocin; D014785:Vision, Ocular
D013311:Streptozocin