Reproductive effects on freshwater fish exposed to 17α-trenbolone and 17α-estradiol.
17α-Trenbolone and 17α-estradiol are principal metabolites in cattle excreta following the administration of Synovex®ONE, which contains trenbolone acetate and estradiol benzoate. As part of the environmental assessment of the use of Synovex®ONE, data were generated to characterize the effects of 17α-trenbolone and 17α-estradiol on the reproduction of freshwater fish. These substances are known endocrine disruptors, so the purpose of testing was not to clarify these properties but to identify concentrations representing population-relevant effects for use in risk characterization. The short-term reproduction assay was conducted with 17α-trenbolone using the fathead minnow (Pimephales promelas) and the medaka (Oryzias latipes) and with 17α-estradiol using the fathead minnow. Adverse effects on the population-relevant endpoints of survival and fecundity were used to establish the no-observed-effect concentration (NOEC) and the lowest-observed-effect concentration (LOEC) for each study. For 17α-trenbolone, adverse effects on fecundity of the fathead minnow occurred at 120 ng/L; this was the LOEC, and the NOEC was 35 ng/L. 17β-Trenbolone did not adversely affect survival and fecundity of medaka at the concentrations tested, resulting in a NOEC of 110 ng/L and a LOEC of >110 ng/L. 17α-Estradiol did not adversely impact survival and fecundity of the fathead minnow at the concentrations tested, resulting in a NOEC and LOEC of 250 ng/L and >250 ng/L, respectively. Environ Toxicol Chem 2017;36:636-644. © 2016 SETAC.
Zoetis, Kalamazoo, Michigan, USA.
Joseph A
JA
Robinson
Exponent, Cary, North Carolina, USA.
Jane P
JP
Staveley
Pfizer Global Research and Development, Groton, Connecticut, USA.
Lisa
L
Constantine
D000818:Animals; D001681:Biological Assay; D002417:Cattle; D003530:Cyprinidae / Q000378:metabolism* / Q000502:physiology; D004784:Environmental Monitoring / Q000379:methods*; D004958:Estradiol / Q000031:analogs & derivatives / Q000378:metabolism / Q000633:toxicity*; D005260:Female; D005618:Fresh Water / Q000737:chemistry; D008297:Male; D009990:Oryzias / Q000378:metabolism* / Q000502:physiology; D012098:Reproduction / Q000187:drug effects*; D014204:Trenbolone Acetate / Q000378:metabolism / Q000633:toxicity*; D014819:Vitellogenins / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*
D014819:Vitellogenins; D014874:Water Pollutants, Chemical; C074283:estradiol 3-benzoate; D004958:Estradiol; D014204:Trenbolone Acetate
Endocrine disruptor; Estradiol; Synovex ONE; Trenbolone; Veterinary medicine