Therapeutic Efficacy of Topically Applied Antioxidant Medicinal Plant Extracts in a Mouse Model of Experimental Dry Eye.
Purpose. To investigate the therapeutic effects of topical administration of antioxidant medicinal plant extracts in a mouse model of experimental dry eye (EDE). Methods. Eye drops containing balanced salt solution (BSS) or 0.001%, 0.01%, and 0.1% extracts were applied for the treatment of EDE. Tear volume, tear film break-up time (BUT), and corneal fluorescein staining scores were measured 10 days after desiccating stress. In addition, we evaluated the levels of interleukin- (IL-) 1β, tumor necrosis factor- (TNF-) α, IL-6, interferon- (IFN-) γ, and IFN-γ associated chemokines, percentage of CD4+C-X-C chemokine receptor type 3 positive (CXCR3+) T cells, goblet cell density, number of 4-hydroxy-2-nonenal (4-HNE) positive cells, and extracellular reactive oxygen species (ROS) production. Results. Compared to the EDE and BSS control groups, the mice treated with topical application of the 0.1% extract showed significant improvements in all clinical parameters, IL-1β, IL-6, TNF-α, and IFN-γ levels, percentage of CD4+CXCR3+ T cells, goblet cell density, number of 4-HNE-positive cells, and extracellular ROS production (P < 0.05). Conclusions. Topical application of 0.1% medicinal plant extracts improved clinical signs, decreased inflammation, and ameliorated oxidative stress marker and ROS production on the ocular surface of the EDE model mice.
Department of Ophthalmology and Center for Creative Biomedical Scientists, Chonnam National University Medical School and Hospital, Gwangju 61469, Republic of Korea.
Won
0000-0002-3720-431X
W
Choi
Department of Dermatology, Chonnam National University Medical School and Hospital, Gwangju 61469, Republic of Korea.
Jee Bum
JB
Lee
Department of Ophthalmology and Center for Creative Biomedical Scientists, Chonnam National University Medical School and Hospital, Gwangju 61469, Republic of Korea.
Lian
0000-0001-7530-8921
L
Cui
Department of Ophthalmology and Center for Creative Biomedical Scientists, Chonnam National University Medical School and Hospital, Gwangju 61469, Republic of Korea.
Ying
Y
Li
Eye Institute of Xiamen University, Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Xiamen, Fujian 361100, China.
Zhengri
0000-0002-1408-6007
Z
Li
Department of Ophthalmology and Center for Creative Biomedical Scientists, Chonnam National University Medical School and Hospital, Gwangju 61469, Republic of Korea.
Ji Suk
JS
Choi
Department of Ophthalmology and Center for Creative Biomedical Scientists, Chonnam National University Medical School and Hospital, Gwangju 61469, Republic of Korea.
Hyo Seok
HS
Lee
Department of Ophthalmology and Center for Creative Biomedical Scientists, Chonnam National University Medical School and Hospital, Gwangju 61469, Republic of Korea.
Kyung Chul
0000-0002-2788-1851
KC
Yoon
D060433:Administration, Ophthalmic; D000447:Aldehydes / Q000378:metabolism; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage*; D000975:Antioxidants / Q000008:administration & dosage*; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005123:Eye / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D005260:Female; D020397:Goblet Cells / Q000187:drug effects / Q000378:metabolism; D018836:Inflammation Mediators / Q000378:metabolism; D008810:Mice, Inbred C57BL; D009883:Ophthalmic Solutions; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage*; D010946:Plants, Medicinal; D017382:Reactive Oxygen Species / Q000378:metabolism; D013666:Tears / Q000187:drug effects* / Q000378:metabolism; D013997:Time Factors; D014985:Xerophthalmia / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology
D000447:Aldehydes; D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D016207:Cytokines; D018836:Inflammation Mediators; D009883:Ophthalmic Solutions; D010936:Plant Extracts; D017382:Reactive Oxygen Species; C027576:4-hydroxy-2-nonenal