A Novel Long-term, Multi-Channel and Non-invasive Electrophysiology Platform for Zebrafish.
Zebrafish are a popular vertebrate model for human neurological disorders and drug discovery. Although fecundity, breeding convenience, genetic homology and optical transparency have been key advantages, laborious and invasive procedures are required for electrophysiological studies. Using an electrode-integrated microfluidic system, here we demonstrate a novel multichannel electrophysiology unit to record multiple zebrafish. This platform allows spontaneous alignment of zebrafish and maintains, over days, close contact between head and multiple surface electrodes, enabling non-invasive long-term electroencephalographic recording. First, we demonstrate that electrographic seizure events, induced by pentylenetetrazole, can be reliably distinguished from eye or tail movement artifacts, and quantifiably identified with our unique algorithm. Second, we show long-term monitoring during epileptogenic progression in a scn1lab mutant recapitulating human Dravet syndrome. Third, we provide an example of cross-over pharmacology antiepileptic drug testing. Such promising features of this integrated microfluidic platform will greatly facilitate high-throughput drug screening and electrophysiological characterization of epileptic zebrafish.
Department of Bioengineering Engineering, University of California, Berkeley, CA 94720, USA.|Berkeley Sensor and Actuator Center, University of California, Berkeley, CA 94720, USA.
SoonGweon
S
Hong
Department of Bioengineering Engineering, University of California, Berkeley, CA 94720, USA.
Philip
P
Lee
Epilepsy Research Laboratory, Department of Neurological Surgery, University of California at San Francisco, USA, CA 94143, USA.|Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California at San Francisco, USA, CA 94143, USA.
Scott C
SC
Baraban
Department of Bioengineering Engineering, University of California, Berkeley, CA 94720, USA.|Berkeley Sensor and Actuator Center, University of California, Berkeley, CA 94720, USA.|Department of Electrical Engineering and Computer Sciences, University of California, Berkeley, CA 94720, USA.|Biophysics Graduate Program, University of California, Berkeley, CA 94720, USA.
Luke P
LP
Lee
D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology / Q000627:therapeutic use; D055724:Electrophysiological Phenomena; D004827:Epilepsy / Q000188:drug therapy / Q000503:physiopathology; D008839:Microelectrodes; D044085:Microfluidics; D015027:Zebrafish / Q000502:physiology*
D000927:Anticonvulsants