Selective Laser Ablation of Methicillin-Resistant Staphylococcus Aureus with IgG Functionalized Multi-Walled Carbon Nanotubes.
Severe infections caused by Methicillin-resistant Staphylococcus aureus (MRSA) and other bacteria are responsible for millions of deaths each year. One of the main objectives of future antibiotic strategies is to develop new anti-infective agents, which would be highly effective and drug-resistant (antimicrobial resistance being currently exhibited by MRSA), using specific antibodies conjugated to thermally active nanomaterials such as NIR-responsive photothermal contrast agents. Multi-walled carbon nanotubes (MWCNTs) covalently functionalized with immunoglobulin G (IgG, an antagonist of Staphylococcal protein A-SpA, which is a MRSA membrane associated protein) were selectively delivered (at various concentrations and incubation times) into MRSA bacteria. Following treatment, cultures were irradiated using an 808 nm 2 w laser diode. The post irradiation death rate ranged from 39.6% (for 1 mg/L) to 79.2% (for 50 mg/L) at 60 seconds (p < 0.001), while at 30 minutes, the death rate increased from 45.2% (1 mg/L) to 85.72% (50 mg/L), p < 0.001. Irradiated MRSAs treated with MWCNTs alone (control) for 60 seconds and 30 minutes, at concentrations ranging from 1 mg/L to 50 mg/L, resulted in significantly lower death rates (7.1-34.1% for 60 seconds, 11.7-48.8% for 30 minutes). Using IgG molecules bound to MWCNTs, followed by laser irradiation, we obtained a very efficacious nanoshell-mediated laser therapy of individual MRSA agents providing highly localized killing effects for IgG-MWCNTs targeted bacteria.
Lucian
L
Mocan
Ioana
I
Ilie
Flaviu A
FA
Tabaran
Cornel
C
Iancu
Ofelia
O
Mosteanu
Teodora
T
Pop
Claudiu
C
Zdrehus
Dana
D
Bartos
Teodora
T
Mocan
Cristian
C
Matea
D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004203:Disinfection / Q000379:methods*; D004307:Dose-Response Relationship, Radiation; D007074:Immunoglobulin G / Q000737:chemistry / Q000378:metabolism*; D053685:Laser Therapy / Q000379:methods*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects*; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D011829:Radiation Dosage; D011838:Radiation-Sensitizing Agents / Q000627:therapeutic use
D007074:Immunoglobulin G; D037742:Nanotubes, Carbon; D011838:Radiation-Sensitizing Agents