Characterization of the complete mitochondrial genome sequence of Homalogaster paloniae (Gastrodiscidae, Trematoda) and comparative analyses with selected digeneans.
Gastrodiscidae species are neglected but significant paramphistomes in small ruminants, which can lead to considerable economic losses to the breeding industry of livestock. However, knowledge about molecular ecology, population genetics, and phylogenetic analysis is still limited. In the present study, we firstly sequenced and analyzed the full mitochondrial (mt) genome of Homalogaster paloniae (14,490 bp). The gene contents and organization of the H. paloniae mt genome is the same as that of other digeneans, such as Fasciola hepatica and Paramphistomum cervi. It is interesting that unlike other paramphistomes, H. paloniae is flat in shape which is similar with Fasciola, such as F. hepatica. Phylogenetic analysis of H. paloniae and other 17 selected digeneans using concatenated amino acid sequences of the 12 protein-coding genes showed that Gastrodiscidae is closely related to Paramphistomidae and Gastrothylacidae. The availability of the mt genome sequence of H. paloniae should provide an important foundation for further molecular study of Gastrodiscidae and other digeneans.
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Xin
X
Yang
Hubei Provincial Center for Diseases Control and Prevention, Wuhan, 430079, Hubei, China.
Lixia
L
Wang
Hubei Entry-Exit Inspection and Quarantine Bureau, Wuhan, 430022, Hubei, China.
Hanli
H
Feng
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Mingwei
M
Qi
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Zongze
Z
Zhang
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Chong
C
Gao
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Chunqun
C
Wang
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Min
M
Hu
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, China. fangrui19810705@163.com.
Rui
R
Fang
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Chengye
C
Li
D000818:Animals; D049751:Genome, Helminth / Q000235:genetics*; D054629:Genome, Mitochondrial / Q000235:genetics*; D058977:Molecular Sequence Annotation; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D014200:Trematoda / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014201:Trematode Infections / Q000469:parasitology*
Homalogaster paloniae; Mitochondrial genome; Phylogenetic analysis