Histopathological and apoptotic changes on marine mussels Mytilus galloprovincialis (Lamark, 1819) following exposure to environmental pollutants.
Marine bivalve mussels, especially Mytilus species are an earlywarning system used for determining of damage caused by the various aquatic pollutions. In the present study, Mytilus galloprovincialis L. (black mussel) have been utilised as a biomonitoring organism to reveal environmental pollution in the Aliaga, Foca and Urla where located along the Izmir Coast of Turkey. Mussels were collected at these areas and gill and hepatopancreas (digestive gland) tissues were excised. mRNA expressions of initiator (caspase-2 and -8) and executioner (caspase -3/7-1, -3/7-2, -3/7-3 and -3/7-4) caspases of mussels tissues in areas exposed to pollution agent have been observed. TUNEL immunoreactivity in paralel to histopathological changes in both Aliaga and Foca areas were compared with Urla. This study is the first report to reveal the pollution with apoptotic expression on mussels in the coast of Turkey.
Ege University, Faculty of Medicine, Department of Histology and Embriology 35100 Bornova-, Izmir, Turkey.
Altuğ
A
Yavaşoğlu
Ege University, Faculty of Science, Department of Biology 35100 Bornova-, Izmir, Turkey.
Dilara
D
Özkan
Ege University, Faculty of Science, Department of Biology 35100 Bornova-, Izmir, Turkey.
Adem
A
Güner
Celal Bayar University, Faculty of Science and Art, Department of Biology Muradiye-, Manisa, Turkey.
Selma
S
Katalay
Ege University, Faculty of Medicine, Department of Histology and Embriology 35100 Bornova-, Izmir, Turkey.
Fatih
F
Oltulu
Ege University, Faculty of Science, Department of Biology 35100 Bornova-, Izmir, Turkey; Ege University, Center for Drug Research & Development and Pharmacokinetic Applications 35100 Bornova-, Izmir, Turkey. Electronic address: ulku.karabay@ege.edu.tr.
N Ülkü Karabay
NÜK
Yavaşoğlu
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001835:Body Weight; D020169:Caspases / Q000235:genetics; D004784:Environmental Monitoring / Q000379:methods*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005880:Gills / Q000473:pathology; D043143:Hepatopancreas / Q000473:pathology; D049878:Mytilus / Q000235:genetics / Q000502:physiology*; D014421:Turkey; D014876:Water Pollution / Q000009:adverse effects* / Q000032:analysis
D020169:Caspases
Mytilus galloprovincialis L.; RT-PCR; TUNEL assay; apoptosis; caspase; histopathology