Wheat alkylresorcinols reduce micellar solubility of cholesterol in vitro and increase cholesterol excretion in mice.
Epidemiological studies have shown that the consumption of whole grains can reduce risk for metabolic disorders. We recently showed that chronic supplementation with wheat alkylresorcinols (ARs) prevents glucose intolerance and insulin resistance with hepatic lipid accumulation induced in mice by a high-fat high-sucrose diet (HFHSD). This study examines the effects of ARs on the micellar solubility of cholesterol in vitro, as well as the effects of transient AR supplementation on faecal lipid excretion and plasma lipid levels in mice. We found that ARs formed bile micelles with taurocholate independently of phospholipids, and dose-dependently decreased the micellar solubility of cholesterol in a biliary micelle model. Transient AR supplementation with HFHSD increased faecal cholesterol and triglyceride contents and decreased plasma cholesterol concentrations. These suggest that one underlying mechanism through which ARs suppress diet-induced obesity is by interfering with the micellar cholesterol solubilisation in the digestive tract, which subsequently decreases cholesterol absorption.
a Biological Clock Research Group , Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST) , Tsukuba , Japan.
Kazumasa
K
Horikawa
a Biological Clock Research Group , Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST) , Tsukuba , Japan.|b Faculty of Science and Technology, Department of Applied Biological Science , Tokyo University of Science , Noda, Chiba , Japan.
Chiaki
C
Hashimoto
c Quality Assurance Division, Research Center for Basic Science, Research and Development , Nisshin Seifun Group Inc. , Fujimino, Saitama , Japan.
Yosuke
Y
Kikuchi
c Quality Assurance Division, Research Center for Basic Science, Research and Development , Nisshin Seifun Group Inc. , Fujimino, Saitama , Japan.
Miki
M
Makita
c Quality Assurance Division, Research Center for Basic Science, Research and Development , Nisshin Seifun Group Inc. , Fujimino, Saitama , Japan.
Shin-Ichi
SI
Fukudome
d Yeast Function Development Unit , Oriental Yeast Co., Ltd. , Itabashi , Japan.
Kimiko
K
Okita
b Faculty of Science and Technology, Department of Applied Biological Science , Tokyo University of Science , Noda, Chiba , Japan.
Naoyuki
N
Wada
a Biological Clock Research Group , Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST) , Tsukuba , Japan.|e Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences , The University of Tokyo , Kashiwa, Chiba , Japan.|f Department of Applied Biological Science, Graduate School of Science and Technology , Tokyo University of Science , Noda, Chiba , Japan.
Katsutaka
K
Oishi
D000818:Animals; D002784:Cholesterol / Q000737:chemistry* / Q000378:metabolism; D019587:Dietary Supplements; D051379:Mice; D008823:Micelles; D012118:Resorcinols / Q000494:pharmacology*; D012995:Solubility; D014280:Triglycerides / Q000378:metabolism; D014908:Triticum / Q000737:chemistry*
D008823:Micelles; D012118:Resorcinols; D014280:Triglycerides; D002784:Cholesterol
ANOVA: analyses of variance; ARs: alkylresorcinols; HFHSD-AR: high-fat high-sucrose diet with 0.4% ARs; HFHSD: high-fat high-sucrose diet; PBS: phosphate-buffered saline; PC: phosphatidylcholine; Wheat alkylresorcinols; bile acids; cholesterol; hepatic lipid; micelle; obesity