[Characterization of the Structure of the Prokaryotic Complex of Antarctic Permafrost by Molecular Genetic Techniques].
A prokaryotic mesophilic organotrophic community responsible for 10% of the total microbial number determined by epifluorescence microscopy was reactivated in the samples ofAntarctic permafrost retrieved from the environment favoring long-term preservation of microbial communities (7500 years). No culturable forms were obtained without resuscitation procedures (CFU = 0). Proteobacteria, Actinobacteria, and Firmicutes were the dominant microbial groups in the complex. Initiation of the reactivated microbial complex by addition of chitin (0.1% wt/vol) resulted in an increased share of metabolically active biomass (up to 50%) due to the functional domination of chitinolytics caused by the target resource. Thus, sequential application of resuscitation procedures and initiation of a specific physiological group (in this case, chitinolytics) to a permafrost-preserved microbial community made it possible to reveal a prokaryotic complex capable of reversion of metabolic activity (FISH data), to determine its phylogenetic structure by metagenomic anal-ysis, and to isolate a pure culture of the dominant microorganism with high chitinolytic activity.
N A
NA
Manucharova
E V
EV
Trosheva
E M
EM
Kol'tsova
E V
EV
Demkina
E V
EV
Karaevskaya
E M
EM
Rivkina
A V
AV
Mardanov
G I
GI
El'-Registan
D000864:Antarctic Regions; D001419:Bacteria / Q000145:classification / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D065946:Permafrost / Q000382:microbiology*; D012988:Soil Microbiology