Association of eNOS and ACE gene polymorphisms and plasma nitric oxide with risk of non-small cell lung cancer in South India.
BACKGROUND
The role of ACE and eNOS gene polymorphisms and their association with various cancers were reported. However, their role in the lung cancer is unclear.


OBJECTIVE
In this study, we analyzed eNOS and ACE gene polymorphisms and the risk of non-small cell lung cancer (NSCLC) in South Indian population.


RESULTS
For the eNOS gene, the homozygous "AA" genotypic frequency was significantly associated with NSCLC with an overall risk of 3.6-fold (P = 0.006, odds ratio = 3.58, 95% confidence interval = 1.66, 7.723). The heterozygous "I/D" genotypic frequency of ACE gene was significantly higher in NSCLC patients when compared to the controls with a 2.29-fold risk for NSCLC. Multiple regression analyses indicated that gender, smoking status, and polymorphisms in eNOS and ACE genes as the strongest predicting factors for an increased susceptibility to NSCLC.


CONCLUSIONS
We report for the first time that polymorphisms in the eNOS "A/A" (homozygous mutant) and ACE "I/D" genotypes might contribute to the increased risk of NSCLC in the South Indian population.
Department of Environmental Toxicology, Institute of Genetics and Hospital for Genetic Diseases, Osmania University, Hyderabad, India.|Present address: Department of Biotechnology, University of Hyderabad, Hyderabad, Andhra Pradesh, 500046, India.
Vidyullatha
V
Peddireddy
Department of Environmental Toxicology, Institute of Genetics and Hospital for Genetic Diseases, Osmania University, Hyderabad, India.
Siva Prasad
SP
Badabagni
Department of Biotechnology, School of Life Sciences, University of Hyderabad, Indo-American Cancer Hospital, Hyderabad, India.
Sandhya Devi
SD
Gundimeda
Department of Environmental Toxicology, Institute of Genetics and Hospital for Genetic Diseases, Osmania University, Hyderabad, India.
Hema Prasad
HP
Mundluru
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D002289:Carcinoma, Non-Small-Cell Lung / Q000453:epidemiology / Q000235:genetics* / Q000378:metabolism; D004247:DNA / Q000191:economics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007194:India / Q000453:epidemiology; D008175:Lung Neoplasms / Q000453:epidemiology / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D052250:Nitric Oxide Synthase Type III / Q000235:genetics* / Q000378:metabolism; D007703:Peptidyl-Dipeptidase A / Q000235:genetics* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012307:Risk Factors
D014408:Biomarkers, Tumor; D004247:DNA; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III; C488034:ACE protein, human; D007703:Peptidyl-Dipeptidase A
angiotensin converting enzyme; gene polymorphism; nitric oxide; nitric oxide synthase; non-small cell lung cancer