Two clinical isolates ofMycoplasma hyosynoviaeshowed differing pattern of lameness and pathogen detection in experimentally challenged pigs.
Mycoplasma (M.) hyosynoviaeis known to colonize and cause disease in growing-finishing pigs. In this study, two clinical isolates ofM. hyosynoviaewere compared by inoculating cesarean-derived colostrum-deprived and specific-pathogen-free growing pigs. After intranasal or intravenous inoculation, the proportion and distribution pattern of clinical cases was compared in addition to the severity of lameness. Tonsils were found to be the primary site of colonization, while bacteremia was rarely detected prior to the observation of clinical signs. Regardless of the clinical isolate, route of inoculation, or volume of inocula, histopathological alterations and tissue invasion were detected in multiple joints, indicating an apparent lack of specific joint tropism. Acute disease was primarily observed 7 to 10 days post-inoculation. The variability in the severity of synovial microscopic lesions and pathogen detection in joint cavities suggests that the duration of joint infection may influence the diagnostic accuracy. In summary, these findings demonstrate that diagnosis ofM. hyosynoviae-associated arthritis can be influenced by the clinical isolate, and provides a study platform to investigate the colonization and virulence potential of field isolates. This approach can be particularly relevant to auxiliate in surveillance and testing of therapeutic and/or vaccine candidates.
Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.|Food Science and Technology, University of Nebraska Lincoln, Lincoln, NE 68588, USA.
João Carlos
JC
Gomes-Neto
Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.|Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI 53703, USA.
Matthew
M
Raymond
Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.
Leslie
L
Bower
Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.
Alejandro
A
Ramirez
Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.
Darin M
DM
Madson
Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.|Merck Animal Health, De Soto, KS 66018, USA.
Erin L
EL
Strait
Zoetis Inc., Global Biologics Research, Kalamazoo, MI 49007, USA.
Everett L
EL
Rosey
Zoetis Inc., Global Biologics Research, Kalamazoo, MI 49007, USA.
Vicki J
VJ
Rapp-Gabrielson
D000208:Acute Disease; D000818:Animals; D001170:Arthritis, Infectious / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D003126:Colostrum; D007794:Lameness, Animal / Q000453:epidemiology* / Q000382:microbiology; D009175:Mycoplasma Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D045791:Mycoplasma hyosynoviae / Q000235:genetics / Q000502:physiology*; D013047:Specific Pathogen-Free Organisms; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000382:microbiology
Mycoplasma hyosynoviae; arthritis; lameness; swine