Surface functionalization and dynamics of polymeric cell culture substrates.
The promise of growing tissues to replace or improve the function of failing ones, a practice often referred to as regenerative medicine, has been driven in recent years by the development of stem cells and cell lines. Stem cells are typically cultured outside the body to increase cell number or differentiate the cells into mature cell types. In order to maximize the regenerative potential of these cells, there is a need to understand cell-material interactions that direct cell behavior and cell-material dynamics. Most synthetic surfaces used for growth and differentiation of cells in the lab are impractical and cost prohibitive in clinical labs. This review focuses on the modification of low cost polymer substrates that are already widely used for cell culture so that they may be used to control and understand cell-material interactions. In addition, we discuss the ability of cells to exert dynamic control over the microenvironment leading to a more complex, less controlled surface.
Department of Biomedical Engineering, University of Wisconsin-Madison, 53706, USA.
John D
JD
Krutty
Department of Materials Science and Engineering, University of Wisconsin-Madison, 53706, USA.
Samantha K
SK
Schmitt
Department of Materials Science and Engineering, University of Wisconsin-Madison, 53706, USA; Department of Chemistry, University of Wisconsin-Madison, 53706, USA.
Padma
P
Gopalan
Department of Biomedical Engineering, University of Wisconsin-Madison, 53706, USA; Department of Materials Science and Engineering, University of Wisconsin-Madison, 53706, USA; Department of Orthopedics and Rehabilitation, University of Wisconsin-Madison, 53706, USA. Electronic address: wlmurphy@wisc.edu.
William L
WL
Murphy
D000818:Animals; D002450:Cell Communication; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D006801:Humans; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D044968:Regenerative Medicine / Q000379:methods*; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
D011108:Polymers