Geniposide alleviates depression-like behavior via enhancing BDNF expression in hippocampus of streptozotocin-evoked mice.
Clinical and preclinical data suggest that diabetes is often psychological complications such as depression. Geniposide (GP), a major compound in Gardenia jasminoides Ellis with both medicinal and nutritional values, has been previously confirmed to exert anti-diabetic and anti-depressive activities. The present study attempted to observe anti-depressive mechanisms of GP in streptozotocin (STZ) evoked diabetic mice by involving brain-derived neurotrophic factor (BDNF), for the first time. Mice were given GP daily (50, and 100 mg/kg, ig) or reference drugs FHMH [fluoxetine hydrochloride (FH, 10 mg/kg, ig) combined with metformin hydrochloride (MH, 100 mg/kg, ig)] for 3 weeks. The forced swimming test (FST) was performed to observe depression-like behavior, and serum and brain tissues were used for neurochemical and fluorescent quantitative reverse transcription PCR analyses. STZ induced excessively increased blood sugar and immobility time in FST, in a manner attenuated by GP and FHMH administration. GP administration further elevated BDNF levels, and up-regulated the mRNA expression of BDNF and tropomyosin-related kinase B (TrkB) in hippocampus of diabetic mice. In addition, STZ induced the excessive level of serum corticosterone (CORT), while GP did not influence on it in diabetic mice. Taken together, these findings indicate that GP can alleviate depression-like behavior in STZ-evoked diabetic mice, and suggest its mechanisms may partially be ascribed to up-regulating BDNF expression in brain.
Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment & Chinese Medicine Development of Henan Province, Henan University of Traditional Chinese Medicine, Zhengzhou, 450046, China. mjw98_2010@163.com.|College of Pharmacy, Henan University of Traditional Chinese Medicine, East Jinshui Road & Boxue Road, Zhengzhou, 450046, China. mjw98_2010@163.com.
Junming
J
Wang
College of Pharmacy, Henan University of Traditional Chinese Medicine, East Jinshui Road & Boxue Road, Zhengzhou, 450046, China.
Peili
P
Duan
Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment & Chinese Medicine Development of Henan Province, Henan University of Traditional Chinese Medicine, Zhengzhou, 450046, China.|College of Pharmacy, Henan University of Traditional Chinese Medicine, East Jinshui Road & Boxue Road, Zhengzhou, 450046, China.
Ying
Y
Cui
College of Pharmacy, Henan University of Traditional Chinese Medicine, East Jinshui Road & Boxue Road, Zhengzhou, 450046, China.
Qing
Q
Li
College of Pharmacy, Henan University of Traditional Chinese Medicine, East Jinshui Road & Boxue Road, Zhengzhou, 450046, China.
Yanran
Y
Shi
D000818:Animals; D000928:Antidepressive Agents / Q000494:pharmacology / Q000627:therapeutic use; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis* / Q000235:genetics; D003863:Depression / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D039823:Iridoids / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D051379:Mice; D011897:Random Allocation
D000928:Antidepressive Agents; D019208:Brain-Derived Neurotrophic Factor; D039823:Iridoids; C007835:geniposide
Antidepressant; Brain-derived neurotrophic factor; Diabetic complications; Forced swimming test; Geniposide