Protocols for Exosome Isolation and RNA Profiling.
Exosomes are small extracellular vesicles of multivesicular bodies derived from the cell endosome. Molecules have a characteristic composition both in the membrane and in the carried load. These particles are located in the culture medium of cells in vitro and in vivo body fluids, being synthesized by almost all kind of cells. The most characteristic molecule transported within is the RNA with a large clinical potential. In this chapter, the methodology for isolation of exosomes and subsequent purification of the RNA is reviewed.
Department of Clinical Biochemistry, University Hospital of Salamanca, Paseo de San Vicente 58, Salamanca, 37007, Spain. f.marg@hotmail.es.|Salamanca Institute for Biomedical Research (IBSAL), Salamanca, Spain. f.marg@hotmail.es.
Fernando
F
Marqués-García
Department of Clinical Biochemistry, University Hospital of Salamanca, Paseo de San Vicente 58, Salamanca, 37007, Spain.|Salamanca Institute for Biomedical Research (IBSAL), Salamanca, Spain.|Department of Medicine, University of Salamanca, Salamanca, Spain.
María
M
Isidoro-García
D000818:Animals; D001826:Body Fluids / Q000378:metabolism; D002478:Cells, Cultured; D002850:Chromatography, Gel / Q000379:methods; D055354:Exosomes / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D010316:Particle Size
Biomarkers; Exosome; Extracellular vesicles; Isolation; Precipitation; RNA profiling; Ultracentrifugation