Functional Analysis of a Type-I Ribosome Inactivating Protein Balsamin from Momordica balsamina with Anti-Microbial and DNase Activity.
Ribosome inactivating proteins (RIPs) have received considerable attention in biomedical research because of their unique activities towards tumor and virus-infected cells. We extracted balsamin, a type-I RIP, from Momordica balsamina. In the present study, a detailed investigation on DNase activity, antioxidant capacity and antibacterial activity was conducted using purified balsamin. DNase-like activity of balsamin towards plasmid DNA was pH, incubation time and temperature dependent. Moreover, the presence of Mg(2+) (10-50 mM) influenced the DNA cleavage activity. Balsamin also demonstrated reducing power and a capacity to scavenge free radicals in a dose dependent manner. Furthermore, the protein exhibited antibacterial activity against Staphylococcus aureus, Salmonella enterica, Staphylococcus epidermidis and Escherichia coli, which suggests potential utility of balsamin as a nutraceutical.
Bioprocessing Laboratory, Centre for Chemistry and Biotechnology, School of Life and Environment Sciences, Deakin University, 75 Pigdons Road, Waurn Ponds, Geelong, 3220, Victoria, Australia.|Metabolic Research Unit, School of Medicine, Deakin University, Waurn Ponds, 75 Pigdons Road, Locked Bag 20000, Geelong, VIC, 3220, Australia.
Parminder Kaur
PK
Ajji
Metabolic Research Unit, School of Medicine, Deakin University, Waurn Ponds, 75 Pigdons Road, Locked Bag 20000, Geelong, VIC, 3220, Australia.
Ken
K
Walder
Bioprocessing Laboratory, Centre for Chemistry and Biotechnology, School of Life and Environment Sciences, Deakin University, 75 Pigdons Road, Waurn Ponds, Geelong, 3220, Victoria, Australia. munish.puri@deakin.edu.au.
Munish
M
Puri
D000900:Anti-Bacterial Agents / Q000032:analysis / Q000494:pharmacology*; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology; D003851:Deoxyribonucleases / Q000037:antagonists & inhibitors*; D004926:Escherichia coli / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000378:metabolism; D029775:Momordica / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology; D010940:Plant Proteins / Q000032:analysis / Q000494:pharmacology*; D054788:Ribosome Inactivating Proteins / Q000032:analysis / Q000494:pharmacology*; D019779:Salmonella enterica / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D013212:Staphylococcus epidermidis / Q000187:drug effects
D000900:Anti-Bacterial Agents; D000975:Antioxidants; D010936:Plant Extracts; D010940:Plant Proteins; C576086:balsamin; D003851:Deoxyribonucleases; D054788:Ribosome Inactivating Proteins; D008274:Magnesium
Antibacterial; Antioxidant; Bioactive; Nutraceutical; Purification; Therapeutic