Inhibitory effects of Schisandra chinensis extract on acne-related inflammation and UVB-induced photoageing.
BACKGROUND
Schisandra chinensis (Turcz.) Baill. (Schisandraceae) fruit extract (SFE) has been reported to induce non-specific tissue protection against inflammation in vivo. However, the effects of SFE on Propionibacterium acnes-stimulated acne and UVB-irradiated photoageing have yet to be investigated.


OBJECTIVE
To systematically investigate the effects of SFE against P. acnes and photoageing in vitro.


METHODS
Qualitative and quantitative analyses of SFE were performed by HPLC. SFE concentrations from 2.5 to 50 μg/mL were tested. Specifically, ELISA was used to examine the levels of pro-inflammatory cytokines in THP-1 cells as well as of collagen I and matrix metalloproteinases-1 in HDF cells. The anti-bacterial effect of SFE was determined using the microdilution broth method. Glutathione and malondialdehyde levels were examined using the colorimetric and TBA methods, respectively. The degree of ageing was determined by cytochemical staining.


RESULTS
SFE significantly inhibited P. acnes growth (MIC 0.5 mg/mL) and 50 μg/mL of SFE suppressed the production of interleukin-1β, interleukin-8 and tumour necrosis factor α, by 59.67%, 62.69% and 68.30%, respectively, in P. acnes-stimulated THP-1 cells. Additionally, 10 μg/mL of SFE suppressed photoageing in UVB-exposed fibroblasts by decreasing metalloproteinase levels by 88.4%, inducing collagen by 58.4% and activating the anti-oxidant defence system, by limiting lipid peroxidation by 51.1% and increasing glutathione production by 34.1% (2.5 μg/mL SFE).


CONCLUSIONS
These results indicated that SFE could significantly ameliorate the inflammatory state in P. acnes-stimulated THP-1 and UVB-irradiated HDF cells, suggesting its potential as a novel agent for acne therapy and photoageing prevention.
a State Key Laboratory of Bioreactor Engineering , East China University of Science and Technology , Shanghai , People's Republic of China.|b Shanghai Collaborative Innovation Center for Biomanufacturing Technology , Shanghai , People's Republic of China.
Miaomiao
M
Guo
a State Key Laboratory of Bioreactor Engineering , East China University of Science and Technology , Shanghai , People's Republic of China.|b Shanghai Collaborative Innovation Center for Biomanufacturing Technology , Shanghai , People's Republic of China.
Yue
Y
Lu
a State Key Laboratory of Bioreactor Engineering , East China University of Science and Technology , Shanghai , People's Republic of China.|b Shanghai Collaborative Innovation Center for Biomanufacturing Technology , Shanghai , People's Republic of China.
Junpeng
J
Yang
c Technology Center, Shanghai Inoherb Cosmetics Co. Ltd. , Shanghai , People's Republic of China.
Xin
X
Zhao
a State Key Laboratory of Bioreactor Engineering , East China University of Science and Technology , Shanghai , People's Republic of China.|b Shanghai Collaborative Innovation Center for Biomanufacturing Technology , Shanghai , People's Republic of China.
Yanhua
Y
Lu
D000152:Acne Vulgaris / Q000188:drug therapy / Q000473:pathology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007249:Inflammation / Q000188:drug therapy / Q000473:pathology; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D011425:Propionibacterium acnes / Q000187:drug effects* / Q000502:physiology; D032141:Schisandra; D015595:Skin Aging / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects*; D014466:Ultraviolet Rays / Q000009:adverse effects*
D010936:Plant Extracts
Anti-inflammatory; UV; anti-photoageing; lignans