[Expression, purification and identification of recombinant Norovirus GII 4 capsid].
OBJECTIVE
The Norovirus GII 4 capsid protein was expressed by baculovirus expression system.


METHODS
The Norovirus GII 4 capsid gene was insert into the HT A plasmid. After sequencing, the recombinant plasmid was transformed into the MAX Efficiency DH10BacT competent cell to express the recombinant capsid protein of Norovirus GII 4. The recombinant capsid protein was purified by Ni-NTA His bind resin affinity chromatography purification column and was identified by Norovirus rapid test kit and SDS-PAGE.


RESULTS
The recombinant protein was successfully expressed with the molecular weight about 60 kD, and it has good immunogenicity.


CONCLUSIONS
The Norovirus GII 4 capsid express plasmid is constructed successfully and the recombinant capsid protein of GII 4 is got in this study.
Jiahui
J
Wang
Chunhui
C
Han
Nan
N
Li
Jin
J
Xu
Fengqin
F
Li
Tao
T
Jiang
D036022:Capsid Proteins / Q000096:biosynthesis*; D029322:Norovirus; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis*
D036022:Capsid Proteins; D011994:Recombinant Proteins