Ribosome Inactivation Leads to Attenuation of Intestinal Polymeric Ig Receptor Expression via Differential Regulation of Human Antigen R.
The polymeric IgR (pIgR) is a central component in the transport of IgA across enterocytes and thereby plays a crucial role in the defense against enteropathogens and in the regulation of circulating IgA levels. The present study was performed to address the novel regulation of pIgR expression in intestinal epithelia undergoing ribosome inactivation. Insults to mucosa that led to ribosome inactivation attenuated pIgR expression in enterocytes. However, IFN regulatory factor-1 (IRF-1) as a central transcription factor of pIgR induction was superinduced by ribosome inactivation in the presence of IFN-γ as a result of mRNA stabilization by the RNA-binding protein HuR. Another important transcription factor for pIgR expression, NF-κB, was marginally involved in suppression of pIgR by ribosome inactivation. In contrast to a positive contribution of HuR in early induction of IRF-1 expression, extended exposure to ribosome inactivation caused nuclear entrapment of HuR, resulting in destabilization of late-phase-induced pIgR mRNA. These HuR-linked differential regulations of pIgR and of IRF-1 led to a reduced mucosal secretion of IgA and, paradoxically, an induction of IRF-1-activated target genes, including colitis-associated IL-7. Therefore, these events can account for ribosome inactivation-related mucosal disorders and provide new insight into interventions for HuR-linked pathogenesis in diverse mucosa-associated diseases, including inflammatory bowel disease and IgA nephritis.
Laboratory of Mucosal Exposome and Biomodulation, Department of Biomedical Sciences, Medical Research Institute, Pusan National University School of Medicine, Yangsan 50612, South Korea; and.
Kee Hun
KH
Do
Laboratory of Mucosal Exposome and Biomodulation, Department of Biomedical Sciences, Medical Research Institute, Pusan National University School of Medicine, Yangsan 50612, South Korea; and.
Seong-Hwan
SH
Park
Laboratory of Mucosal Exposome and Biomodulation, Department of Biomedical Sciences, Medical Research Institute, Pusan National University School of Medicine, Yangsan 50612, South Korea; and Research Institute for Basic Sciences, Immunoregulatory Therapeutics Group in Brain, Busan 21 Project, Yangsan 50612, South Korea.
Juil
J
Kim
Laboratory of Mucosal Exposome and Biomodulation, Department of Biomedical Sciences, Medical Research Institute, Pusan National University School of Medicine, Yangsan 50612, South Korea; and Research Institute for Basic Sciences, Immunoregulatory Therapeutics Group in Brain, Busan 21 Project, Yangsan 50612, South Korea.
Mira
M
Yu
Laboratory of Mucosal Exposome and Biomodulation, Department of Biomedical Sciences, Medical Research Institute, Pusan National University School of Medicine, Yangsan 50612, South Korea; and Research Institute for Basic Sciences, Immunoregulatory Therapeutics Group in Brain, Busan 21 Project, Yangsan 50612, South Korea moon@pnu.edu.
Yuseok
Y
Moon
D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D004195:Disease Models, Animal; D000067780:ELAV-Like Protein 1 / Q000378:metabolism*; D020895:Enterocytes / Q000378:metabolism; D054308:Enteropathogenic Escherichia coli; D004927:Escherichia coli Infections / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D006801:Humans; D018928:Immunity, Mucosal / Q000502:physiology*; D007070:Immunoglobulin A / Q000276:immunology / Q000378:metabolism; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D007413:Intestinal Mucosa / Q000378:metabolism*; D051379:Mice; D018613:Microscopy, Confocal; D016133:Polymerase Chain Reaction; D019056:Receptors, Polymeric Immunoglobulin / Q000096:biosynthesis*; D012270:Ribosomes / Q000378:metabolism*
D000067780:ELAV-Like Protein 1; D007070:Immunoglobulin A; D019056:Receptors, Polymeric Immunoglobulin