17 β-estradiol mineralization in human waste products and soil in the presence and the absence of antimicrobials.
Natural steroidal estrogens, such as 17 β-estradiol (E2), as well as antimicrobials such as doxycycline and norfloxacin, are excreted by humans and hence detected in sewage sludge and biosolid. The disposal of human waste products on agricultural land results in estrogens and antibiotics being detected as mixtures in soils. The objective of this study was to examine microbial respiration and E2 mineralization in sewage sludge, biosolid, and soil in the presence and the absence of doxycycline and norfloxacin. The antimicrobials were applied to the media either alone or in combination at total rates of 4 and 40 mg kg-1, with the 4 mg kg-1rate being an environmentally relevant concentration. The calculated time that half of the applied E2 was mineralized ranged from 294 to 418 days in sewage sludge, from 721 to 869 days in soil, and from 2,258 to 14,146 days in biosolid. E2 mineralization followed first-order and the presence of antimicrobials had no significant effect on mineralization half-lives, except for some antimicrobial applications to the human waste products. At 189 day, total E2 mineralization was significantly greater in sewage sludge (38 ±0.7%) > soil (23 ±0.7%) > biosolid (3 ±0.7%), while total respiration was significantly greater in biosolid (1,258 mg CO2) > sewage sludge (253 mg CO2) ≥ soil (131 mg CO2). Strong sorption of E2 to the organic fraction in biosolid may have resulted in reduced E2 mineralization despite the high microbial activity in this media. Total E2 mineralization at 189 day was not significantly influenced by the presence of doxycycline and/or norfloxacin in the media. Antimicrobial additions also did not significantly influence total respiration in media, except that total CO2respiration at 189 day was significantly greater for biosolid with 40 mg kg-1doxycycline added, relative to biosolid without antimicrobials. We conclude that it is unlikely for doxycycline and norfloxacin, or their mixtures, to have a significant effect on E2 mineralization in human waste products and soil. However, the potential for E2 to be persistent in biosolids, with and without the presence of antimicrobials, is posing a challenge for biosolid disposal to agricultural lands.
a Department of Soil Science , University of Manitoba , Winnipeg , Manitoba , Canada.
Inoka
I
Amarakoon
a Department of Soil Science , University of Manitoba , Winnipeg , Manitoba , Canada.
Annemieke
A
Farenhorst
a Department of Soil Science , University of Manitoba , Winnipeg , Manitoba , Canada.
Karin
K
Rose
b Department of Biology , Institut Universitaire de Technologie de Perpignan , Perpignan, Pyrénées Orientales , France.
Anne
A
Claeys
c Department of Pharmacy , Universidade Federal dos Vales do Jequitinhonha e Mucuri , Diamantina , Minas Gerais , Brazil.
Bruna
B
Ascef
D000383:Agriculture; D000890:Anti-Infective Agents / Q000032:analysis / Q000494:pharmacology*; D004958:Estradiol / Q000032:analysis / Q000378:metabolism*; D005243:Feces / Q000737:chemistry; D006801:Humans; D008350:Manitoba; D064307:Microbiota / Q000187:drug effects*; D012722:Sewage / Q000032:analysis / Q000382:microbiology*; D012987:Soil / Q000737:chemistry*; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism*; D014556:Urine / Q000737:chemistry; D014866:Waste Products / Q000032:analysis*
D000890:Anti-Infective Agents; D012722:Sewage; D012987:Soil; D012989:Soil Pollutants; D014866:Waste Products; D004958:Estradiol
17 β-estradiol; antimicrobial; biosolid; doxycycline; mineralization; norfloxacin; respiration; sewage sludge; soil