Insulin and 20-hydroxyecdysone action in Bombyx mori: Glycogen content and expression pattern of insulin and ecdysone receptors in fat body.
Insulin and ecdysone signaling play a critical role on the growth and development of insects including Bombyx mori. Our previous study showed that Bombyx larvae reached critical weight for metamorphosis between day 3.5 and 4 of the fifth larval instar. The present study showed that the effect of insulin on the accumulation of glycogen in fat body of Bombyx larvae depends on the critical growth period. When larvae are in active growth period (before reaching critical weight), insulin caused increased accumulation of glycogen, while its treatment in larvae at terminal growth period (after critical period) resulted in an increased mobilization of glycogen. During terminal growth period, insulin and 20-hydroxyecdysone (20E) showed an antagonistic effect on the accumulation of fat body glycogen in fed, food deprived and decapitated larvae as well as in isolated abdomens. Insulin treatment decreased the glycogen content, whereas, 20E increased it. Food deprivation and decapitation caused an increase in the transcript levels of insulin receptor (InR) and this increase in InR expression might be attributed to a decrease in synthesis/secretion of insulin-like peptides, as insulin treatment in these larvae showed a down-regulation in InR expression. However, insulin showed an up-regulation in InR in isolated abdomens and it suggests that in food deprived and decapitated larvae, the exogenous insulin may interact with some head and/or thoracic factors in modulating the expression of InR. Moreover, in fed larvae, insulin-mediated increase in InR expression indicates that its regulation by insulin-like peptides also depends on the nutritional status of the larvae. The treatment of 20E in fed larvae showed an antagonistic effect on the transcript levels since a down-regulation in InR expression was observed. 20E treatment also led to a decreased expression of InR in food deprived and decapitated larvae as well as in isolated abdomens. Insulin and 20E also modulated the expression level of ecdysone receptors (EcRB1 and USP1). 20E treatment showed an up-regulation in expression of ecdysone receptors, but only in fed larvae, whereas insulin treatment showed a down-regulation in the expression of EcRB1 and USP1 in all the experimental larvae studied. Further, the data indicates that an up-regulation of ecdysone receptors is associated with an increase in fat body glycogen content, whereas an up-regulation of insulin receptor expression causes glycogen mobilization. The study, therefore, suggests that the insulin and ecdysone signaling are linked to each other and that both insulin and ecdysone are involved in regulating the carbohydrate reserves in B. mori.
Department of Zoology, North-Eastern Hill University, Shillong 793022, Meghalaya, India. Electronic address: bkeshan@hotmail.com.
Bela
B
Keshan
Department of Zoology, North-Eastern Hill University, Shillong 793022, Meghalaya, India.
Bembem
B
Thounaojam
Department of Zoology, North-Eastern Hill University, Shillong 793022, Meghalaya, India.
Sanathoibi D
SD
Kh
D000818:Animals; D012831:Bombyx / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D004440:Ecdysone / Q000378:metabolism; D004441:Ecdysterone / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D005216:Fat Body / Q000378:metabolism*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006003:Glycogen / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D007814:Larva; D008675:Metamorphosis, Biological / Q000187:drug effects / Q000235:genetics; D011972:Receptor, Insulin / Q000235:genetics / Q000378:metabolism; D011987:Receptors, Steroid / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics
D007328:Insulin; D011987:Receptors, Steroid; C055469:ecdysone receptor; D004440:Ecdysone; D004441:Ecdysterone; D006003:Glycogen; D011972:Receptor, Insulin
Critical weight; Decapitation; Fat body; Food deprivation; Isolated abdomen; Terminal growth period