Bioprocess development for kefiran production by Lactobacillus kefiranofaciens in semi industrial scale bioreactor.
Lactobacillus kefiranofaciens is non-pathogenic gram positive bacteria isolated from kefir grains and able to produce extracellular exopolysaccharides named kefiran. This polysaccharide contains approximately equal amounts of glucose and galactose. Kefiran has wide applications in pharmaceutical industries. Therefore, an approach has been extensively studied to increase kefiran production for pharmaceutical application in industrial scale. The present work aims to maximize kefiran production through the optimization of medium composition and production in semi industrial scale bioreactor. The composition of the optimal medium for kefiran production contained sucrose, yeast extract and K2HPO4 at 20.0, 6.0, 0.25 g L(-1), respectively. The optimized medium significantly increased both cell growth and kefiran production by about 170.56% and 58.02%, respectively, in comparison with the unoptimized medium. Furthermore, the kinetics of cell growth and kefiran production in batch culture of L. kefiranofaciens was investigated under un-controlled pH conditions in 16-L scale bioreactor. The maximal cell mass in bioreactor culture reached 2.76 g L(-1) concomitant with kefiran production of 1.91 g L(-1).
Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), 81130 UTM, Skudai, Malaysia.
Daniel Joe
DJ
Dailin
Bioproducts Research Chair, Zoology Department, Faculty of Science, King Saud University, 11451 Riyadh, Saudi Arabia; Natural and Microbial Products Department, National Research Centre, Dokki, Cairo, Egypt.
Elsayed Ahmed
EA
Elsayed
Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), 81130 UTM, Skudai, Malaysia.
Nor Zalina
NZ
Othman
Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), 81130 UTM, Skudai, Malaysia.
Roslinda
R
Malek
Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), 81130 UTM, Skudai, Malaysia.
Hiew Siaw
HS
Phin
Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), 81130 UTM, Skudai, Malaysia.
Ramlan
R
Aziz
Bioproducts Research Chair, Zoology Department, Faculty of Science, King Saud University, 11451 Riyadh, Saudi Arabia.
Mohamad
M
Wadaan
Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), 81130 UTM, Skudai, Malaysia; City of Scientific Research and Technology Application, New Burg Al Arab, Alexandria, Egypt.
Hesham Ali
HA
El Enshasy
Kefiran; Lactobacillus kefiranofaciens; Medium optimization; Polysaccharide production; Stirred tank bioreactor; Submerged culture