IL-33 enhances retinoic acid signaling on CD4+ T cells.
Several molecules have been described as CD4+ T cells differentiation modulators and among them retinoic acid (RA) and more recently, IL-33, have been studied. Due to the similarities in T helper cell skewing properties between RA and IL-33, we asked whether IL-33 intersects, directly or indirectly, the RA signaling pathway. Total CD4+ T cells from DR5-luciferase mice were activated in the presence of RA with or without IL-33, and RA signaling was visualized using ex vivo imaging. Our results demonstrate that IL-33 itself is able to trigger RA signaling on CD4+ T cells, which is highly increased when IL-33 is added in conjunction with RA. This study presents IL-33 as a potential player that may synergize with RA in controlling T cell differentiation, and suggests that IL-33 may be an attractive target in controlling T cell differentiation in vivo.
Centro de Investigación Biomédica (CIB), Facultad de Medicina, Universidad de los Andes, Santiago, Chile.
Tania
T
Gajardo
ICBM, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
Francisco
F
Pérez
Centro de Investigación Biomédica (CIB), Facultad de Medicina, Universidad de los Andes, Santiago, Chile.
Claudia
C
Terraza
Centro de Investigación Biomédica (CIB), Facultad de Medicina, Universidad de los Andes, Santiago, Chile.
Mauricio
M
Campos-Mora
Geisel Medical School, Dartmouth College, NH, United States.
Randolph J
RJ
Noelle
Centro de Investigación Biomédica (CIB), Facultad de Medicina, Universidad de los Andes, Santiago, Chile. Electronic address: kpino@uandes.cl.
Karina
K
Pino-Lagos
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D000067596:Interleukin-33 / Q000378:metabolism*; D051379:Mice; D015398:Signal Transduction / Q000502:physiology*; D014212:Tretinoin / Q000378:metabolism*
C506535:Il33 protein, mouse; D000067596:Interleukin-33; D014212:Tretinoin
CD4+ T cells differentiation; IL-33; Retinoic acid