Life cycle inference and phylogeny of Ortholinea labracis n. sp. (Myxosporea: Ortholineidae), a parasite of the European seabass Dicentrarchus labrax (Teleostei: Moronidae), in a Portuguese fish farm.
Ortholinea labracis n. sp. is described and its life cycle is inferred from a Southern Portuguese fish farm, with basis on microscopic and molecular procedures. This myxosporean parasite infects the urinary bladder of the European seabass Dicentrarchus labrax and the intestinal epithelium of a marine oligochaete of the genus Tectidrilus. Myxospores subspherical in valvular view and ellipsoidal in sutural view measuring 7.6 ± 0.3 (6.8-8.7) μm in length, 7.2 ± 0.2 (6.7-7.7) μm in width and 6.5 ± 0.4 (5.8-7.7) μm in thickness. Two polar capsules, 3.0 ± 0.2 (2.6-3.4) μm long and 2.4 ± 0.1 (2.0-2.9) μm wide, located at the same level, but with divergent orientation and opening to opposite sides of the suture line. Sequencing of the SSU rRNA gene revealed a similarity of 100% between the analysed myxospores and triactinomyxon actinospores. The phylogenetic setting of O. labracis n. sp. shows subgrouping in correlation with tissue tropism, but identifies this parasite as another exception to the main division of Myxosporea into the main freshwater and marine lineages.
Laboratory of Animal Pathology, Department of Biology, Faculty of Sciences, University of Porto, Porto, Portugal.|Interdisciplinary Centre of Marine and Environmental Research (CIIMAR/CIMAR), University of Porto, Porto, Portugal.
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Rangel
Interdisciplinary Centre of Marine and Environmental Research (CIIMAR/CIMAR), University of Porto, Porto, Portugal.|Laboratory of Cell Biology, Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Porto, Portugal.
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Rocha
Interdisciplinary Centre of Marine and Environmental Research (CIIMAR/CIMAR), University of Porto, Porto, Portugal.|Department of Sciences, University Institute of Health Sciences, CESPU, Gandra, Portugal.
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Casal
Interdisciplinary Centre of Marine and Environmental Research (CIIMAR/CIMAR), University of Porto, Porto, Portugal.
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Castro
Interdisciplinary Centre of Marine and Environmental Research (CIIMAR/CIMAR), University of Porto, Porto, Portugal.
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Severino
Interdisciplinary Centre of Marine and Environmental Research (CIIMAR/CIMAR), University of Porto, Porto, Portugal.|Laboratory of Cell Biology, Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Porto, Portugal.|Zoology Department, College of Sciences, King Saud University, Riyadh, Saudi Arabia.
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Azevedo
Laboratory of Animal Pathology, Department of Biology, Faculty of Sciences, University of Porto, Porto, Portugal.|Interdisciplinary Centre of Marine and Environmental Research (CIIMAR/CIMAR), University of Porto, Porto, Portugal.
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Cavaleiro
Laboratory of Animal Pathology, Department of Biology, Faculty of Sciences, University of Porto, Porto, Portugal.|Interdisciplinary Centre of Marine and Environmental Research (CIIMAR/CIMAR), University of Porto, Porto, Portugal.
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Santos
D000818:Animals; D001492:Bass; D004275:DNA, Ribosomal / Q000235:genetics; D005393:Fish Diseases / Q000453:epidemiology* / Q000469:parasitology; D007413:Intestinal Mucosa / Q000469:parasitology; D046529:Microscopy, Electron, Transmission / Q000662:veterinary; D055764:Myxozoa / Q000145:classification / Q000502:physiology*; D009835:Oligochaeta / Q000469:parasitology*; D010273:Parasitic Diseases, Animal / Q000453:epidemiology* / Q000469:parasitology; D011174:Portugal / Q000453:epidemiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000662:veterinary; D001743:Urinary Bladder / Q000469:parasitology
D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
SSU rRNA gene; Tectidrilus sp; inferred life cycle; morphology; phylogeny; triactinomyxon