Gold Nanoparticles-Based Barcode Analysis for Detection of Norepinephrine.
Nanotechnology-based bio-barcode amplification analysis offers an innovative approach for detecting neurotransmitters. We evaluated the efficacy of this method for detecting norepinephrine in normal and oxidative-stress damaged dopaminergic cells. Our approach use a combination of DNA barcodes and bead-based immunoassays for detecting neurotransmitters with surface-enhanced Raman spectroscopy (SERS), and provides polymerase chain reaction (PCR)-like sensitivity. This method relies on magnetic Dynabeads containing antibodies and nanoparticles that are loaded both with DNA barcords and with antibodies that can sandwich the target protein captured by the Dynabead-bound antibodies. The aggregate sandwich structures are magnetically separated from the solution and treated to remove the conjugated barcode DNA. The DNA barcodes are then identified by SERS and PCR analysis. The concentration of norepinephrine in dopaminergic cells can be readily detected using the bio-barcode assay, which is a rapid, high-throughput screening tool for detecting neurotransmitters.
Jeung Hee
JH
An
Kwon-Jai
KJ
Lee
Jeong-Woo
JW
Choi
D017304:Annexin A5 / Q000378:metabolism; D017209:Apoptosis; D045744:Cell Line, Tumor; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D015335:Molecular Probes / Q000138:chemical synthesis / Q000737:chemistry; D009638:Norepinephrine / Q000032:analysis*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D011419:Propidium / Q000378:metabolism; D015203:Reproducibility of Results; D013059:Spectrum Analysis, Raman
D017304:Annexin A5; D015335:Molecular Probes; D011419:Propidium; D006046:Gold; D016650:Fluorescein-5-isothiocyanate; D009638:Norepinephrine