Expression of an alfalfa (Medicago sativa L.) peroxidase gene in transgenic Arabidopsis thaliana enhances resistance to NaCl and H2O2.
Peroxidases (PODs) are enzymes that play important roles in catalyzing the reduction of H2O2 and the oxidation of various substrates. They function in many different and important biological processes, such as defense mechanisms, immune responses, and pathogeny. The POD genes have been cloned and identified in many plants, but their function in alfalfa (Medicago sativa L.) is not known, to date. Based on the POD gene sequence (GenBank accession No. L36157.1), we cloned the POD gene in alfalfa, which was named MsPOD. MsPOD expression increased with increasing H2O2. The gene was expressed in all of the tissues, including the roots, stems, leaves, and flowers, particularly in stems and leaves under light/dark conditions. A subcellular analysis showed that MsPOD was localized outside the cells. Transgenic Arabidopsis with MsPOD exhibited increased resistance to H2O2 and NaCl. Moreover, POD activity in the transgenic plants was significantly higher than that in wild-type Arabidopsis. These results show that MsPOD plays an important role in resistance to H2O2 and NaCl.
Turfgrass Research Institute, College of Forestry, Beijing Forestry University, Beijing, China.
K
K
Teng
The College of Horticulture and Garden, Yangtze University, Jingzhou, China.
G Z
GZ
Xiao
Turfgrass Research Institute, College of Forestry, Beijing Forestry University, Beijing, China.
W E
WE
Guo
Turfgrass Research Institute, College of Forestry, Beijing Forestry University, Beijing, China.
J B
JB
Yuan
Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot, China.
J
J
Li
Turfgrass Research Institute, College of Forestry, Beijing Forestry University, Beijing, China.
Y H
YH
Chao
Turfgrass Research Institute, College of Forestry, Beijing Forestry University, Beijing, China.
L B
LB
Han
D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D035264:Flowers / Q000201:enzymology / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000633:toxicity; D000455:Medicago sativa / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D018384:Oxidative Stress / Q000235:genetics; D009195:Peroxidase / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D055049:Salt Tolerance / Q000235:genetics; D012965:Sodium Chloride / Q000633:toxicity; D013312:Stress, Physiological / Q000235:genetics
D012965:Sodium Chloride; D006861:Hydrogen Peroxide; D009195:Peroxidase