Continous application of bioorganic fertilizer induced resilient culturable bacteria community associated with banana Fusarium wilt suppression.
Fusarium wilt of banana always drives farmers to find new land for banana cultivation due to the comeback of the disease after a few cropping years. A novel idea for solving this problem is the continuous application of bioorganic fertilizer (BIO), which should be practiced from the beginning of banana planting. In this study, BIO was applied in newly reclaimed fields to pre-control banana Fusarium wilt and the culturable rhizobacteria community were evaluated using Biolog Ecoplates and culture-dependent denaturing gradient gel electrophoresis (CD-DGGE). The results showed that BIO application significantly reduced disease incidences and increased crop yields, respectivly. And the stabilized general bacterial metabolic potential, especially for the utilization of carbohydrates, carboxylic acids and phenolic compounds, was induced by BIO application. DGGE profiles demonstrated that resilient community structure of culturable rhizobacteria with higher richness and diversity were observed in BIO treated soils. Morever, enriched culturable bacteria affiliated with Firmicutes, Gammaproteobacteria and Actinobacteria were also detected. In total, continuous application of BIO effectively suppressed Fusarium wilt disease by stabilizing culturable bacterial metabolic potential and community structure. This study revealed a new method to control Fusarium wilt of banana for long term banana cultivation.
Jiangsu Key Lab for Organic Solid Waste Utilization, National Engineering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University, Nanjing, China.
Lin
L
Fu
Hainan key Laboratory for Sustainable Utilization of Tropical Bio-resources, College of Agriculture, Hainan University, Haikou, China.
Yunze
Y
Ruan
Jiangsu Key Lab for Organic Solid Waste Utilization, National Engineering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University, Nanjing, China.
Chengyuan
C
Tao
Jiangsu Key Lab for Organic Solid Waste Utilization, National Engineering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University, Nanjing, China.
Rong
R
Li
Jiangsu Key Lab for Organic Solid Waste Utilization, National Engineering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University, Nanjing, China.
Qirong
Q
Shen
D000383:Agriculture; D000818:Animals; D001419:Bacteria / Q000145:classification / Q000187:drug effects / Q000254:growth & development*; D002244:Carbon / Q000494:pharmacology; D058645:Denaturing Gradient Gel Electrophoresis; D005308:Fertilizers; D005670:Fusarium / Q000502:physiology*; D014644:Genetic Variation; D007700:Kinetics; D008372:Manure; D028521:Musa / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology*; D025341:Principal Component Analysis; D058441:Rhizosphere; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D034421:Sus scrofa
D005308:Fertilizers; D008372:Manure; D012987:Soil; D002244:Carbon