Identification of structurally closely related monosaccharide and disaccharide isomers by PMP labeling in conjunction with IM-MS/MS.
It remains particularly difficult for gaining unambiguous information on anomer, linkage, and position isomers of oligosaccharides using conventional mass spectrometry (MS) methods. In our laboratory, an ion mobility (IM) shift strategy was employed to improve confidence in the identification of structurally closely related disaccharide and monosaccharide isomers using IMMS. Higher separation between structural isomers was achieved using 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization in comparison with phenylhydrazine (PHN) derivatization. Furthermore, the combination of pre-IM fragmentation of PMP derivatives provided sufficient resolution to separate the isomers not resolved in the IMMS. To chart the structural variation observed in IMMS, the collision cross sections (CCSs) for the corresponding ions were measured. We analyzed nine disaccharide and three monosaccharide isomers that differ in composition, linkages, or configuration. Our data show that coexisting carbohydrate isomers can be identified by the PMP labeling technique in conjunction with ion-mobility separation and tandem mass spectrometry. The practical application of this rapid and effective method that requires only small amounts of sample is demonstrated by the successful analysis of water-soluble ginseng extract. This demonstrated the potential of this method to measure a variety of heterogeneous sample mixtures, which may have an important impact on the field of glycomics.
Changchun University of Chinese Medicine, Changchun 130117, China.|Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022, China.
Hongmei
H
Yang
High Temperature Reactor Holdings Co., Ltd., China Nuclear Engineering Group Co., Beijing 100037, China.
Lei
L
Shi
Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022, China.
Xiaoyu
X
Zhuang
Changchun University of Chinese Medicine, Changchun 130117, China.
Rui
R
Su
Department of Entomology and Comprehensive Cancer Center, University of California, Davis, CA 95616, United States.
Debin
D
Wan
Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022, China.
Fengrui
F
Song
High Temperature Reactor Holdings Co., Ltd., China Nuclear Engineering Group Co., Beijing 100037, China.
Jinying
J
Li
Changchun University of Chinese Medicine, Changchun 130117, China.
Shuying
S
Liu
D000983:Antipyrine / Q000031:analogs & derivatives* / Q000737:chemistry; D004187:Disaccharides / Q000032:analysis*; D000077553:Edaravone; D016166:Free Radical Scavengers / Q000737:chemistry; D009005:Monosaccharides / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D004187:Disaccharides; D016166:Free Radical Scavengers; D009005:Monosaccharides; D000077553:Edaravone; D000983:Antipyrine