The Effect of MCP-1/CCR2 on the Proliferation and Senescence of Epidermal Constituent Cells in Solar Lentigo.
Solar lentigo (SL) is a representative photoaging skin disorder. Alteration of the main epidermal constituent cells-keratinocytes and melanocytes-in relation to the photoaged dermal environment or chemokine/cytokine network is suggested as its pathogenesis. Among these, we focused on monocyte chemoattractant protein-1 (MCP-1), as it is known to be associated with tissue aging. For the first time, we report that the MCP-1 receptor, CCR2, is expressed in normal human melanocytes. In SL tissue, there was an increase of CCR2+Melan A+ melanocytes with positivity to Rb protein compared to peri-lesional normal skin. MCP-1 induced the proliferation of normal human melanocytes without a significant change in the melanin content. MCP-1 treatment in normal human keratinocytes showed an increase in senescence-associated β-galactosidase staining and p53 and p21 protein expressions. In summary, MCP-1 may participate in the development of SL by affecting epidermal constituent cells, for example, by inducing melanocyte proliferation and keratinocyte senescence.
Department of Dermatology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Korea. uucm79@hanmail.net.
Woo Jin
WJ
Lee
Department of Dermatology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Korea. sooyounyda@gmail.com.
Soo Youn
SY
Jo
Department of Dermatology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Korea. algp_@naver.com.
Mi Hye
MH
Lee
Department of Dermatology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Korea. chwon98@chol.com.
Chong Hyun
CH
Won
Department of Dermatology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Korea. miumiu@amc.seoul.kr.
Mi Woo
MW
Lee
Department of Dermatology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Korea. jhchoy@amc.seoul.kr.
Jee Ho
JH
Choi
Department of Dermatology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Korea. csesnumd@gmail.com.
Sung Eun
SE
Chang
D000368:Aged; D015415:Biomarkers; D001706:Biopsy; D049109:Cell Proliferation / Q000528:radiation effects; D002470:Cell Survival / Q000235:genetics / Q000528:radiation effects; D016922:Cellular Senescence / Q000235:genetics / Q000528:radiation effects; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D000078404:Epidermal Cells; D004817:Epidermis / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D007911:Lentigo / Q000188:drug therapy / Q000209:etiology* / Q000378:metabolism* / Q000473:pathology; D008544:Melanocytes / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects; D008875:Middle Aged; D013472:Sunlight / Q000009:adverse effects*; D001616:beta-Galactosidase / Q000378:metabolism
D015415:Biomarkers; D018932:Chemokine CCL2; D001616:beta-Galactosidase
epidermal constituent cells; monocyte chemoattractant protein-1; solar lentigo