Effects of fasting on serial measurements of hyperpolarized [1-(13) C]pyruvate metabolism in tumors.
Imaging of the metabolism of hyperpolarized [1-(13) C]pyruvate has shown considerable promise in preclinical studies in oncology, particularly for the assessment of early treatment response. The repeatability of measurements of (13) C label exchange between pyruvate and lactate was determined in a murine lymphoma model in fasted and non-fasted animals. The fasted state showed lower intra-individual variability, although the [1-(13) C]lactate/[1-(13) C]pyruvate signal ratio was significantly greater in fasted than in non-fasted mice, which may be explained by the higher tumor lactate concentrations in fasted animals. These results indicate that the fasted state may be preferable for the measurement of (13) C label exchange between pyruvate and lactate, as it reduces the variability and therefore should make it easier to detect the effects of therapy. © 2016 The Authors. NMR in Biomedicine published by John Wiley & Sons Ltd.
Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Cambridge, UK.|Department of Biochemistry, University of Cambridge, Cambridge, UK.
Eva M
EM
Serrao
Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Cambridge, UK.|Department of Biochemistry, University of Cambridge, Cambridge, UK.
Tiago B
0000-0002-4474-0639
TB
Rodrigues
Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Cambridge, UK.|Department of Radiology, University of Cambridge, Cambridge Biomedical Campus, Cambridge, UK.
Ferdia A
FA
Gallagher
Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Cambridge, UK.|Department of Biochemistry, University of Cambridge, Cambridge, UK.|A. I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, Kuopio, Finland.
Mikko I
0000-0002-2004-660X
MI
Kettunen
Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Cambridge, UK.|Department of Biochemistry, University of Cambridge, Cambridge, UK.
Brett W C
BW
Kennedy
Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Cambridge, UK.
Sarah L
SL
Vowler
Core Biochemical Assay Laboratory, Cambridge University Hospitals NHS Foundation Trust, Cambridge, UK.
Keith A
KA
Burling
Cancer Research UK Cambridge Institute, University of Cambridge, Li Ka Shing Centre, Cambridge, UK.|Department of Biochemistry, University of Cambridge, Cambridge, UK.
Kevin M
KM
Brindle
D000465:Algorithms; D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism*; D066241:Carbon-13 Magnetic Resonance Spectroscopy / Q000379:methods*; D005215:Fasting / Q000378:metabolism*; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D009374:Neoplasms, Experimental / Q000378:metabolism* / Q000473:pathology; D019289:Pyruvic Acid / Q000378:metabolism*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012815:Signal Processing, Computer-Assisted
D014408:Biomarkers, Tumor; D019289:Pyruvic Acid
cancer; fasting; hyperpolarization; lymphoma; pyruvate; repeatability