A highly versatile and easily configurable system for plant electrophysiology.
In this study we present a highly versatile and easily configurable system for measuring plant electrophysiological parameters and ionic flow rates, connected to a computer-controlled highly accurate positioning device. The modular software used allows easy customizable configurations for the measurement of electrophysiological parameters. Both the operational tests and the experiments already performed have been fully successful and rendered a low noise and highly stable signal. Assembly, programming and configuration examples are discussed. The system is a powerful technique that not only gives precise measuring of plant electrophysiological status, but also allows easy development of ad hoc configurations that are not constrained to plant studies. •We developed a highly modular system for electrophysiology measurements that can be used either in organs or cells and performs either steady or dynamic intra- and extracellular measurements that takes advantage of the easiness of visual object-oriented programming.•High precision accuracy in data acquisition under electrical noisy environments that allows it to run even in a laboratory close to electrical equipment that produce electrical noise.•The system makes an improvement of the currently used systems for monitoring and controlling high precision measurements and micromanipulation systems providing an open and customizable environment for multiple experimental needs.
Lab. Fisiología Vegetal, Facultad Biociencias, Universidad Autónoma de Barcelona, E-08193 Bellaterra, Spain.
Benet
B
Gunsé
Lab. Fisiología Vegetal, Facultad Biociencias, Universidad Autónoma de Barcelona, E-08193 Bellaterra, Spain.
Charlotte
C
Poschenrieder
Helmholtz Zentrum München, Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH), Ingolstädter Landstr. 1, 85764 Neuherberg, Germany.
Simone
S
Rankl
Helmholtz Zentrum München, Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH), Ingolstädter Landstr. 1, 85764 Neuherberg, Germany.
Peter
P
Schröeder
Department of Plant, Soil and Environmental Science, Polo Scientifico, Viale delle Idee 30, 50019 SestoFiorentino, Firenze, Italy.
Ana
A
Rodrigo-Moreno
Lab. Fisiología Vegetal, Facultad Biociencias, Universidad Autónoma de Barcelona, E-08193 Bellaterra, Spain.
Juan
J
Barceló
Electrophysiology; Improved system for plant electrophysiology; Ion flux measurement; Membrane potential; Plant root