High-flexibility combinatorial peptide synthesis with laser-based transfer of monomers in solid matrix material.
Laser writing is used to structure surfaces in many different ways in materials and life sciences. However, combinatorial patterning applications are still limited. Here we present a method for cost-efficient combinatorial synthesis of very-high-density peptide arrays with natural and synthetic monomers. A laser automatically transfers nanometre-thin solid material spots from different donor slides to an acceptor. Each donor bears a thin polymer film, embedding one type of monomer. Coupling occurs in a separate heating step, where the matrix becomes viscous and building blocks diffuse and couple to the acceptor surface. Furthermore, we can consecutively deposit two material layers of activation reagents and amino acids. Subsequent heat-induced mixing facilitates an in situ activation and coupling of the monomers. This allows us to incorporate building blocks with click chemistry compatibility or a large variety of commercially available non-activated, for example, posttranslationally modified building blocks into the array's peptides with >17,000 spots per cm(2).
Karlsruhe Institute of Technology, Institute of Microstructure Technology (IMT), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
Felix F
FF
Loeffler
Karlsruhe Institute of Technology, Institute of Microstructure Technology (IMT), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
Tobias C
TC
Foertsch
Karlsruhe Institute of Technology, Institute of Microstructure Technology (IMT), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
Roman
R
Popov
Karlsruhe Institute of Technology, Institute of Microstructure Technology (IMT), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
Daniela S
DS
Mattes
Karlsruhe Institute of Technology, Institute for Inorganic Chemistry, Engesserstrasse 15, 76131 Karlsruhe, Germany.|Karlsruhe Institute of Technology, Institute of Nanotechnology (INT), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
Martin
M
Schlageter
Karlsruhe Institute of Technology, Institute of Microstructure Technology (IMT), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
Martyna
M
Sedlmayr
Karlsruhe Institute of Technology, Institute of Microstructure Technology (IMT), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
Barbara
B
Ridder
Florian-Xuan
FX
Dang
Karlsruhe Institute of Technology, Institute of Microstructure Technology (IMT), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
Clemens
C
von Bojničić-Kninski
Karlsruhe Institute of Technology, Institute of Microstructure Technology (IMT), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
Laura K
LK
Weber
Karlsruhe Institute of Technology, Institute of Microstructure Technology (IMT), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
Andrea
A
Fischer
Karlsruhe Institute of Technology, Institute of Microstructure Technology (IMT), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
Juliane
J
Greifenstein
Karlsruhe Institute of Technology, Institute of Microstructure Technology (IMT), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
Valentina
V
Bykovskaya
Technical University of Varna, 1 Studentska, 9010 Varna, Bulgaria.
Ivan
I
Buliev
German Cancer Research Center, INF 580, 69120 Heidelberg, Germany.
F Ralf
FR
Bischoff
Karlsruhe Institute of Technology, Institute of Microstructure Technology (IMT), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
Lothar
L
Hahn
Michael A R
MA
Meier
Karlsruhe Institute of Technology, Institute of Toxicology and Genetics (ITG), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
Stefan
S
Bräse
Karlsruhe Institute of Technology, Institute for Inorganic Chemistry, Engesserstrasse 15, 76131 Karlsruhe, Germany.|Karlsruhe Institute of Technology, Institute of Nanotechnology (INT), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
Annie K
0000-0003-3944-7427
AK
Powell
Teodor Silviu
0000-0003-4614-2461
TS
Balaban
Karlsruhe Institute of Technology, Institute of Microstructure Technology (IMT), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
Frank
F
Breitling
Karlsruhe Institute of Technology, Institute of Microstructure Technology (IMT), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
Alexander
A
Nesterov-Mueller
D002234:Carbodiimides / Q000737:chemistry; D020650:Combinatorial Chemistry Techniques; D005449:Fluorenes / Q000737:chemistry; D006388:Hemagglutinins / Q000737:chemistry; D062425:Hydroxybenzoate Ethers / Q000737:chemistry; D007834:Lasers; D008689:Methacrylates / Q000737:chemistry; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D060327:Solid-Phase Synthesis Techniques / Q000379:methods*
D002234:Carbodiimides; D005449:Fluorenes; D006388:Hemagglutinins; D062425:Hydroxybenzoate Ethers; D008689:Methacrylates; D009842:Oligopeptides; C524499:polyethylene glycol methacrylate; D011092:Polyethylene Glycols; C081611:1,3-diisopropylcarbodiimide; C085706:FLAG peptide