Expression of nestin, CD133 and ABCG2 in relation to the clinical outcome in pediatric sarcomas.
BACKGROUND
Nestin, CD133 and ABCG2 are recently discussed as putative markers, co-expression of which might determine a cancer stem cell (CSC) phenotype in sarcomas.


OBJECTIVE
Our study is focused on immunohistochemical analysis of nestin, CD133 and ABCG2 expression in rhabdomyosarcoma, Ewing sarcoma and osteosarcoma. Furthermore, we also analyzed the possible correlation of nestin, CD133 and ABCG2 expression levels with the patient outcome to identify potential prognostic values of these three putative CSC markers in the same cohorts.


METHODS
Using immunohistochemistry, expression of nestin, CD133 and ABCG2 was analyzed in 24 rhabdomyosarcoma, 22 Ewing sarcoma and 10 osteosarcoma tissue samples and expression levels of these markers were correlated with clinical outcome.


RESULTS
High nestin levels indicate poor prognosis in patients with Ewing sarcoma (P = 0.001), and high CD133 expression is associated with shorter survival in rhabdomyosarcoma patients (P = 0.002). In contrast, no significant relationship was found between ABCG2 expression and the clinical outcome.


CONCLUSIONS
Our analysis represents the first complex study of these three putative CSCs markers together in three different types of pediatric sarcomas and showed their possible prognostic values in these tumors.
Department of Pathological Anatomy, Medical Faculty, Masaryk University and St. Anne's University Hospital, Brno, Czech Republic.|International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic.
Iva
I
Zambo
Department of Pathological Anatomy, Medical Faculty, Masaryk University and St. Anne's University Hospital, Brno, Czech Republic.
Marketa
M
Hermanova
Department of Pediatric Oncology, Medical Faculty, Masaryk University and University Hospital Brno, Brno, Czech Republic.
Danica
D
Zapletalova
Department of Pediatric Oncology, Medical Faculty, Masaryk University and University Hospital Brno, Brno, Czech Republic.|Laboratory of Tumor Biology, Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic.|International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic.
Jan
J
Skoda
Department of Pediatric Oncology, Medical Faculty, Masaryk University and University Hospital Brno, Brno, Czech Republic.
Peter
P
Mudry
Department of Pediatric Oncology, Medical Faculty, Masaryk University and University Hospital Brno, Brno, Czech Republic.|International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic.
Michal
M
Kyr
Department of Pediatric Oncology, Medical Faculty, Masaryk University and University Hospital Brno, Brno, Czech Republic.
Karel
K
Zitterbart
Department of Pediatric Oncology, Medical Faculty, Masaryk University and University Hospital Brno, Brno, Czech Republic.
Jaroslav
J
Sterba
Department of Pediatric Oncology, Medical Faculty, Masaryk University and University Hospital Brno, Brno, Czech Republic.|Laboratory of Tumor Biology, Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic.|International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic.
Renata
R
Veselska
D000071916:AC133 Antigen / Q000235:genetics / Q000378:metabolism*; D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2 / Q000235:genetics / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D014408:Biomarkers, Tumor; D002648:Child; D002675:Child, Preschool; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D007231:Infant, Newborn; D053208:Kaplan-Meier Estimate; D008297:Male; D060787:Neoplasm Grading; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D014411:Neoplastic Stem Cells / Q000378:metabolism; D064231:Nestin / Q000235:genetics / Q000378:metabolism*; D011379:Prognosis; D012509:Sarcoma / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism* / Q000401:mortality*; D055815:Young Adult
D000071916:AC133 Antigen; D000070997:ATP Binding Cassette Transporter, Subfamily G, Member 2; D014408:Biomarkers, Tumor; D064231:Nestin
Ewing sarcoma; Rhabdomyosarcoma; cancer stem cell markers; osteosarcoma; prognosis