Effect of exopolysaccharide from Ganoderma applanatum on the electrical properties of mouse fibroblast cells line L929 culture using an electric cell-substrate impedance sensing (ECIS) - Preliminary study.
IIntroduction and objective. In recent years there has been intensified research on medicinal preparations of fungal origin. Some fungal polysaccharides may directly affect the inhibition of cancer cells proliferation which, stopping the cell cycle, leads to apoptosis. One of these substances (component of extract of Ganoderma spp) is extensively tested for its anti-cancer properties on the tumor cell lines. Electric cell-substrate impedance sensing (ECIS) is an in vitro impedance measuring system using alternating current (AC) to determinate the behaviour of the cells in physiological conditions.


OBJECTIVE
The aim of the study was to examine the electric properties (resistance, capacitance and impedance) of mouse fibroblasts cell line L929 after treatment by different concentration of crude exopolysaccharides from Ganoderma applanatum (GpEPS) in real time by ECIS technique.


METHODS
For the study, the L929 cell line culture was treated by different concentrations of GpEPS: C1=228.5 µg/mL; C2=22.85 µg/mL; C3=2.285 µg/mL; C4=0.2285 µg/mL; and C5=0.02285 µg/mL. Default optimal frequencies were used: Resistance (R) 4000Hz, Impedance (Z) 16000Hz, Capacitance (C) 64000Hz.


RESULTS
The study demonstrated that GpEPS had no significant effect on the resistance, capacitance and impedance cells cultures, which implies that there is no significant effect on the physiological processes of L929 fibroblasts. This indicates the possibility of using GpEPS preparation in anti-cancer therapy.


CONCLUSIONS
In the future, following further studies (comprising in preventive and therapeutic actions), GpEPS can be safely used in anti-cancer therapy which does not cause side-effects or damage to healthy cells.
Physiology Department, Medical University of Lublin, Poland.
Monika
M
Prendecka
Physiology Department, Medical University of Lublin, Poland.
Radosław
R
Mlak
Department Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland.
Magdalena
M
Jaszek
Department Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland.
Monika
M
Osińska-Jaroszuk
Physiology Department, Medical University of Lublin, Poland.
Monika
M
Jakubiak-Hulicz
ibidi GmbH Am Klopferspitz 19 D-82152 Martinsried (München) Germany.
Christian
C
Leibold
ibidi GmbH Am Klopferspitz 19 D-82152 Martinsried (München) Germany.
Armin
A
Bieser
Institute of Electronics and Information Technology Technical University of Lublin, Poland.
Waldemar
W
Wójcik
Physiology Department, Medical University of Lublin, Poland.
Teresa
T
Małecka-Massalska
D000818:Animals; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D017097:Electric Impedance; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D062610:Fungal Polysaccharides / Q000737:chemistry*; D032902:Ganoderma / Q000737:chemistry*; D051379:Mice
D062610:Fungal Polysaccharides