Promoter Assay Using Luciferase Reporter Gene in the A549 Cell Line.
The development of reporters systems has simplified the study of promoter activity in different areas of knowledge, and represents an easy and fast approach to study genetic variations. In this chapter, we show a transfection protocol of A549 lung epithelial cells with a reporter vector, using the Luciferase-Renilla dual system for studying the variations caused by several polymorphisms in the promoter region of a gene.
Department of Clinical Biochemistry, University Hospital of Salamanca, Paseo de San Vicente 58, Salamanca, 37007, Spain. elemarcos@hotmail.com.|Salamanca Institute for Biomedical Research (IBSAL), Salamanca, Spain. elemarcos@hotmail.com.
Elena
E
Marcos-Vadillo
IBSAL, Salamanca Institute for Biomedical Research, Salamanca, Spain.|Department of Biomedical and Diagnostic Sciences, University of Salamanca, Spain.
Asunción
A
García-Sánchez
D000072283:A549 Cells; D003001:Cloning, Molecular; D017930:Genes, Reporter; D006801:Humans; D049410:Luciferases, Renilla / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D014162:Transfection / Q000379:methods*
D049410:Luciferases, Renilla
Luciferase; Promoter activity; Renilla; Reporter; Transfection