Cyclic nucleotide-gated channel 18 functions as an essential Ca2+channel for pollen germination and pollen tube growth in Arabidopsis.
We recently revealed that cyclic nucleotide-gated channel 18 (CNGC18) functioned as the main Ca2+channel in pollen tube tips for pollen tube guidance to ovules by regulating external Ca2+influx in Arabidopsis. In this study, we found that the reduction of external Ca2+concentration ([Ca2+]ext) from 10 mM to 5 mM, and further to 2 mM, led to the decreases of pollen germination percentages, but led to the increases of the percentages of ruptured pollen grains and tubes, and branched pollen tubes in vitro in cngc18-17 compared with wild type. The second point mutant allele cngc18-22 showed similar phenotypes, including reduced pollen germination percentages, increased percentages of ruptured pollen tubes, but did not show obvious different percentages of ruptured pollen grains and branched pollen tubes compared with wild type. These data demonstrate that CNGC18 plays essential roles in pollen germination and tube growth as a Ca2+channel in Arabidopsis.
a National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences , Chinese Academy of Sciences , Shanghai , China.|b University of Chinese Academy of Sciences , Shanghai , China.
Li-Li
LL
Gu
a National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences , Chinese Academy of Sciences , Shanghai , China.|b University of Chinese Academy of Sciences , Shanghai , China.|c School of Life Sciences , Shaanxi Normal University , Xi'an , China.
Qi-Fei
QF
Gao
a National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences , Chinese Academy of Sciences , Shanghai , China.
Yong-Fei
YF
Wang
D017360:Arabidopsis / Q000378:metabolism* / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D054815:Cyclic Nucleotide-Gated Cation Channels / Q000235:genetics / Q000378:metabolism*; D018525:Germination / Q000235:genetics / Q000502:physiology*; D011058:Pollen / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D053205:Pollen Tube / Q000235:genetics / Q000378:metabolism / Q000502:physiology
D029681:Arabidopsis Proteins; C000589714:CNGC18 protein, Arabidopsis; D054815:Cyclic Nucleotide-Gated Cation Channels
Arabidopsis; CNGC18; pollen germination; pollen tube growth