Zein as biodegradable material for effective delivery of alkaline phosphatase and substrates in biokits and biosensors.
A biodegradable material, zein, is proposed as a reagent delivery platform for biokits and biosensors based on alkaline phosphatase (ALP) activity/inhibition in the presence of phosphatase substrates. The immobilization and release of both the substrate and/or the active ALP, in a biodegradable and low-cost material such as zein, a prolamin from maize, and in combination with glycerol as plasticizer have been investigated. Three zein-based devices are proposed for several applications: (1) inorganic phosphorus estimation in water of different sources (river, lake, coastal water and tap water) with a detection limit of 0.2mg/L - compared to at least 1mg/L required by legislation, (2) estimation of ALP in saliva and (3) chlorpyrifos control in commercial preparations. The single-use kits developed are low cost, easy and fast to manufacture and are stable for at least 20 days at -20°C, so the zein film can preserve and deliver both the enzyme and substrates.
MINTOTA group, Departament de Química Analítica, Facultat de Química, Universitat of València, Dr. Moliner 50, 46100 Burjassot, Valencia, Spain.
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Jornet-Martínez
MINTOTA group, Departament de Química Analítica, Facultat de Química, Universitat of València, Dr. Moliner 50, 46100 Burjassot, Valencia, Spain. Electronic address: pilar.campins@uv.es.
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Campíns-Falcó
Institute of Biotechnology, Department of Chemical Engineering and Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, United Kingdom. Electronic address: lisa.hall@biotech.cam.ac.uk.
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Hall
D020341:Absorbable Implants; D065966:Absorption, Physicochemical; D000469:Alkaline Phosphatase / Q000008:administration & dosage / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation; D003124:Colorimetry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D010758:Phosphorus / Q000032:analysis*; D010968:Plasticizers / Q000737:chemistry; D011933:Reagent Kits, Diagnostic; D013050:Spectrometry, Fluorescence / Q000295:instrumentation; D015028:Zein / Q000737:chemistry*
D001672:Biocompatible Materials; D010968:Plasticizers; D011933:Reagent Kits, Diagnostic; D010758:Phosphorus; D015028:Zein; D000469:Alkaline Phosphatase
Alkaline phosphatase; Biomaterial; Biosensors; Pesticide; Saliva; Zein