Molecular cloning of IgZ heavy chain isotype in Catla catla and comparative expression profile of IgZ and IgM following pathogenic infection.
Immunoglobulins serve as a crucial arm of the adaptive immune system against detrimental pathogenic threats in teleosts. However, whether the novel Ig isotype IgZ is present in the Indian major carp, Catla catla, has not yet been elucidated. The present study reports the presence of IgZ ortholog in C. catla (CcIgZ) and further demonstrates its comparative tissue specific expression with IgM (CcIgM) in response to bacterial and parasitic stimulation. The putative 139 amino acid sequence of IgZ heavy chain cDNA of C. catla showed homology with IgZ constant domains of other teleosts. Phylogenetic analysis of the predicted IgZ transcript sequence clustered with previously identified IgZ heavy chain sequences of Cyprinidae family members. The inductive expression profiles of IgZ and IgM genes were evaluated in immunologically relevant tissues at 24, 48 and 72 hr post infection with Aeromonas hydrophila, Streptococcus uberis and Argulus sp. Both CcIgZ and CcIgM were expressed most strongly in the kidneys of healthy fish. Basal expression of CcIgM transcript was higher than that of CcIgZ in all the examined tissues. Stimulation with bacteria triggered significant increase of IgZ in the intestine (P < 0.001) and spleen (P < 0.01), whereas IgM was relatively up-regulated in blood (P < 0.001) after stimulation with each of the three pathogens assessed. The study is the first to report identification of IgZ in C. catla. Further, it provides insights into the differential expression profiles of IgZ and IgM isotypes against various pathogenic infection in C. catla, which may facilitate better prophylaxis again such infections.
Laboratory of Environmental Microbiology and Ecology, Department of Life Science, National Institute of Technology, Rourkela 769 008.
Bhakti
B
Patel
Laboratory of Environmental Microbiology and Ecology, Department of Life Science, National Institute of Technology, Rourkela 769 008.
Rajanya
R
Banerjee
Fish Health Management Division, Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar 751002, Odisha, India.
Madhubanti
M
Basu
Fish Health Management Division, Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar 751002, Odisha, India.
Saswati
S
Lenka
Fish Health Management Division, Central Institute of Freshwater Aquaculture, Kausalyaganga, Bhubaneswar 751002, Odisha, India.
Mrinal
M
Samanta
Laboratory of Environmental Microbiology and Ecology, Department of Life Science, National Institute of Technology, Rourkela 769 008.
Surajit
S
Das
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D005393:Fish Diseases / Q000235:genetics* / Q000276:immunology / Q000382:microbiology / Q000469:parasitology; D005399:Fishes / Q000235:genetics* / Q000276:immunology; D015870:Gene Expression; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006790:Host-Parasite Interactions / Q000235:genetics / Q000276:immunology; D054884:Host-Pathogen Interactions / Q000235:genetics / Q000276:immunology; D007143:Immunoglobulin Heavy Chains / Q000737:chemistry / Q000235:genetics*; D007132:Immunoglobulin Isotypes / Q000737:chemistry / Q000235:genetics*; D007075:Immunoglobulin M / Q000737:chemistry / Q000235:genetics*; D010802:Phylogeny; D059467:Transcriptome
D007143:Immunoglobulin Heavy Chains; D007132:Immunoglobulin Isotypes; D007075:Immunoglobulin M
Catla catla; IgZ; pathogen; tissue expression