Efficient production of propionic acid through high density culture with recycling cells of Propionibacterium acidipropionici.
The aim of this study was to explore propionic acid production via high density culture of Propionibacterium acidipropionici and recycling of cells. Results showed that final cells of P. acidipropionici from high density culture still had high metabolic activity for reuse. Using our process, 75.9gl(-1) propionic acid was produced, which was 1.84-fold of that in fed-batch fermentation with low cell density (41.2gl(-1)); the corresponding productivity was 100.0% higher than that in fed-batch fermentation with low cell density (0.16gl(-1)h(-1)). This bioprocess may have potential for the industrial production of propionic acid.
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China; Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, People's Republic of China.
Zhen
Z
Liu
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
Yongsheng
Y
Ge
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
Jing
J
Xu
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
Chao
C
Gao
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China. Electronic address: macq@sdu.edu.cn.
Cuiqing
C
Ma
State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.
Ping
P
Xu
D018929:Cell Culture Techniques / Q000379:methods*; D018914:Cells, Immobilized / Q000378:metabolism; D011422:Propionates / Q000138:chemical synthesis*; D011424:Propionibacterium / Q000378:metabolism
D011422:Propionates; C029658:propionic acid
Cell density; Productivity; Propionibacterium acidipropionici; Propionic acid