Molecular-level evaluation of selected periodontal pathogens from subgingival regions in canines and humans with periodontal disease.
Dogs commonly serve as a model for various human conditions, including periodontal diseases. The aim of this study was to identify the anaerobic bacteria that colonize the subgingival areas in dogs and humans by using rapid real-time polymerase chain reaction (RT-PCR)-based tests and to compare the results obtained in each species. Bacterial microflora evaluations, both quantitative and qualitative, were performed by applying ready-made tests on twelve dogs and twelve humans. Five samples were collected from each subject's deepest gingival pockets and joined to form a collective sample. The results of the study revealed interspecies similarities in the prevalences ofPorphyromonas(P.)gingivalis, Treponema denticola, Tannerella forsythia, andFusobacterium nucleatum. Red complex bacteria comprised the largest portion of the studied bacterial complexes in all study groups, withP. gingivalisbeing the most commonly isolated bacterium. The results show similarities in the prevalence of bacterial microflora in dogs and humans. Microbiological analysis of gingival pockets by using rapid real-time PCR-based tests in clinical practice, both veterinary and human, can facilitate the choice of appropriate pharmacological treatment and can provide a basis for subsequent verification of the treatment's effectiveness.
Department and Clinic of Animal Surgery, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-612 Lublin, Poland.
Magdalena
M
Gołyńska
Department and Clinic of Animal Surgery, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-612 Lublin, Poland.
Izabela
I
Polkowska
Dental Center 'Lardent', 20-135 Lublin, Poland.
Małgorzata
M
Bartoszcze-Tomaszewska
Department and Clinic of Animal Surgery, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-612 Lublin, Poland.
Aleksandra
A
Sobczyńska-Rak
Children's Orthopaedic Clinic and Rehabilitation Department, Medical University of Lublin, 20-093 Lublin, Poland.
Łukasz
Ł
Matuszewski
D000328:Adult; D000818:Animals; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics; D004283:Dog Diseases / Q000453:epidemiology* / Q000382:microbiology; D004285:Dogs; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010510:Periodontal Diseases / Q000453:epidemiology* / Q000382:microbiology / Q000662:veterinary*; D011044:Poland / Q000453:epidemiology; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary
D004269:DNA, Bacterial
anaerobic bacteria; dogs; humans; periodontal diseases; real-time polymerase chain reaction