Effects of Homoserine Dehydrogenase Deficiency on Production of Cytidine by Mutants of Bacillus subtilis.
A homoserine-requiring mutant, Bacillus subtilis strain No. 615, was isolated from a cytidine-producing mutant strain No. 515. The homoserine dehydrogenase activity of strain No. 615 was reduced to less than 1/50 of that of No. 515. Strain No. 615 accumulated 23.5 mg/ml cytidine in a medium containing 16% glucose, and strain No. 515 accumulated 18.8 mg/ml cytidine under the same conditions. The effects of glucose concentration on cytidine production were examined, and strain No. 615 accumulated 30.2 mg/ml cytidine in a medium containing 20% glucose.
a Fermentation Center, Technology Development Laboratories , Takeda Chemical Industries, Ltd. , Yodogawa-ku, Osaka , 532 , Japan.
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Asahi
a Fermentation Center, Technology Development Laboratories , Takeda Chemical Industries, Ltd. , Yodogawa-ku, Osaka , 532 , Japan.
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Tsunemi
a Fermentation Center, Technology Development Laboratories , Takeda Chemical Industries, Ltd. , Yodogawa-ku, Osaka , 532 , Japan.
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Doi
Bacillus subtilis; cytidine; homoserine dehydrogenase; pyrimidine nucleotide(s)