Specific Adsorption of Clostridium stercorarium Xylanase to Amorphous Cellulose and Its Desorption by Cellobiose.
Clostidium stercorarium xylanase A (XynA) composed of a family 11 catalytic domain of glycosyl hydrolases and family VI CBDs bound to amorphous cellulose, i.e., acid-swollen cellulose (ASC), but not highly crystalline cellulose, and it was released from the cellulose protein complex by wash with a cellobiose solution. The Ka and [PC]max values of ASC were 0.25 liter/μmol and 26μmol/g.
a Department of Bioscience, Faculty of Bioresources Mie University , Tsu 514 , Japan.
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Goro
a Department of Bioscience, Faculty of Bioresources Mie University , Tsu 514 , Japan.|b Center for Molecular Biology and Genetics , Mie University , Tsu 514 , Japan.
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Shuichi
a Department of Bioscience, Faculty of Bioresources Mie University , Tsu 514 , Japan.
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Asako
a Department of Bioscience, Faculty of Bioresources Mie University , Tsu 514 , Japan.
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Md Mainul
a Department of Bioscience, Faculty of Bioresources Mie University , Tsu 514 , Japan.
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Tetsuya
a Department of Bioscience, Faculty of Bioresources Mie University , Tsu 514 , Japan.
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Kazuo
a Department of Bioscience, Faculty of Bioresources Mie University , Tsu 514 , Japan.
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Kunio
Clostridium stercorarium; cellulose-binding domain; xylanase