Instrument Quality Control.
Well-defined quality control (QC) processes are used to determine whether a certain procedure or action conforms to a widely accepted standard and/or set of guidelines, and are important components of any laboratory quality assurance program (Popa-Burke et al., J Biomol Screen 14: 1017-1030, 2009). In this chapter, we describe QC procedures useful for monitoring the accuracy and precision of laboratory instrumentation, most notably automated liquid dispensers. Two techniques, gravimetric QC and photometric QC, are highlighted in this chapter. When used together, these simple techniques provide a robust process for evaluating liquid handler accuracy and precision, and critically underpin high-quality research programs.
Warp Drive Bio, 400 Technology Square, 2nd Floor, Cambridge, MA, 02139, USA. cjayakody@warpdrivebio.com.
Chatura
C
Jayakody
Center for Integrative Chemical Biology and Drug Discovery, UNC Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, NC, 27599-7363, USA.
Emily A
EA
Hull-Ryde
D000465:Algorithms; D004396:Coloring Agents / Q000032:analysis; D057166:High-Throughput Screening Assays / Q000295:instrumentation* / Q000379:methods / Q000592:standards; D010783:Photometry / Q000295:instrumentation* / Q000379:methods / Q000592:standards; D011786:Quality Control; D012984:Software; D013645:Tartrazine / Q000032:analysis; D014894:Weights and Measures / Q000295:instrumentation* / Q000592:standards
D004396:Coloring Agents; D013645:Tartrazine
Assay performance; Gravimetric quality control; High-throughput screening QC; Liquid-handling variability; Photometric quality control; Quality control