Changes in the Fatty Acid Profile and Phospholipid Molecular Species Composition of Human Erythrocyte Membranes after Hybrid Palm and Extra Virgin Olive Oil Supplementation.
This work aims to evaluate and compare, for the first time, the effects of extra virgin olive oil (EVOO) and hybrid palm oil (HPO) supplementation on the fatty acid profile and phospholipid (PL) molecular species composition of human erythrocyte membranes. Results supported the effectiveness of both HPO and EVOO supplementation (3 months, 25 mL/day) in decreasing the lipophilic index of erythrocytes with no significant differences between HPO and EVOO groups at month 3. On the other hand, the novel and rapid ultraperformance liquid chromatography-tandem mass spectrometry method used for PL analysis reveals an increase in the levels of phosphatidylcholine and phosphatidylethanolamine species esterified with polyunsaturated fatty acids. This work demonstrates the ability of both EVOO and HPO to increase the degree of unsaturation of erythrocyte membrane lipids with an improvement in membrane fluidity that could be associated with a lower risk of developing cardiovascular diseases.
Department of Agricultural, Food, and Environmental Sciences, Polytechnic University of Marche , Via Brecce Bianche, 60131 Ancona, Italy.
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D
Pacetti
Department of Agricultural, Food, and Environmental Sciences, Polytechnic University of Marche , Via Brecce Bianche, 60131 Ancona, Italy.
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R
Gagliardi
Department of Agricultural, Food, and Environmental Sciences, Polytechnic University of Marche , Via Brecce Bianche, 60131 Ancona, Italy.
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M
Balzano
Department of Agricultural, Food, and Environmental Sciences, Polytechnic University of Marche , Via Brecce Bianche, 60131 Ancona, Italy.
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Frega
Department of Nutrition and Biochemistry, Faculty of Sciences, Pontificia Universidad Javeriana , KR 7 #40-62, Bogota D.C., Columbia.
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Ojeda
Department of Nutrition and Biochemistry, Faculty of Sciences, Pontificia Universidad Javeriana , KR 7 #40-62, Bogota D.C., Columbia.
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M
Borrero
Department of Internal Medicine, School of Medicine, Pontificia Universidad Javeriana , KR 7 #40-62, Bogota D.C., Columbia.
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A
Ruiz
Department of Agri-food, Environmental and Animal Sciences, University of Udine , Via Sondrio 2/a, 33100 Udine, Italy.
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Lucci
D019587:Dietary Supplements / Q000032:analysis; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism*; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008563:Membrane Lipids / Q000737:chemistry / Q000378:metabolism; D000069463:Olive Oil / Q000378:metabolism*; D000073878:Palm Oil; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism*; D010938:Plant Oils / Q000378:metabolism*
D005227:Fatty Acids; D008563:Membrane Lipids; D000069463:Olive Oil; D010743:Phospholipids; D010938:Plant Oils; D000073878:Palm Oil
dietary supplementation; erythrocytes; extra virgin olive oil; functional oils; hybrid palm oil; phospholipids