Characterization of a polyhydroxyalkanoate obtained from pineapple peel waste using Ralsthonia eutropha.
Agro-industrial waste can be the production source of biopolymers such as polyhydroxyalkanoates. The aim of this study was to produce and characterize Polyhydroxyalkanoates produced from pineapple peel waste fermentation processes. The methodology includes different pineapple peel waste fermentation conditions. The produced biopolymer was characterized using FTIR, GC-MS and NMR. The best fermentation condition for biopolymer production was obtained using pH 9, Carbon/Nitrogen 11, carbon/phosphorus 6 and fermentation time of 60h. FTIR analyzes showed PHB group characteristics, such as OH, CH and CO. In addition, GC-MS showed two monomers with 4 and 8 carbons, referred to PHB and PHBHV. H(1) NMR analysis showed 0.88-0.97 and 5.27ppm signals, corresponding to CH3 and CH, respectively. In conclusion, polyhydroxyalkanoate production from pineapple peels waste is an alternative for the treatment of waste generated in Colombia's fruit industry.
BIOALI Research Group, Department of Food, Faculty of Pharmaceutical Sciences and Food, Universidad de Antioquia, Medellin, Calle 67 No. 53-108, Colombia. Electronic address: oscar.vega@udea.edu.co.
Oscar
O
Vega-Castro
BIOALI Research Group, Department of Food, Faculty of Pharmaceutical Sciences and Food, Universidad de Antioquia, Medellin, Calle 67 No. 53-108, Colombia. Electronic address: jose.contrerasc@udea.edu.co.
Jose
J
Contreras-Calderon
BIOALI Research Group, Department of Food, Faculty of Pharmaceutical Sciences and Food, Universidad de Antioquia, Medellin, Calle 67 No. 53-108, Colombia. Electronic address: leonflorian1989@gmail.com.
Emilson
E
León
Grupo Investigación Catalizadores y Absorbentes, Universidad de Antioquia, Medellín, Calle 67 No. 53-108, Colombia. Electronic address: almirsegura@gmail.com.
Almir
A
Segura
Universidad Nacional de Colombia - Sede Medellín, Medellín, Calle 59ª No.63-20, Colombia. Electronic address: marioari@unal.edu.co.
Mario
M
Arias
Universidad Nacional de Colombia - Sede Bogotá, Bogotá, Carrera 45 No.26-85, Colombia. Electronic address: ldperezp@unal.edu.co.
León
L
Pérez
Universidad de São Paulo (USP), Av. Duque de Caxias Norte, 225, 13635-900, Pirassununga, SP, Brazil, Brazil. Electronic address: pjsobral@usp.br.
Paulo J A
PJA
Sobral
D031230:Ananas / Q000737:chemistry*; D020118:Cupriavidus necator / Q000378:metabolism*; D005285:Fermentation; D008401:Gas Chromatography-Mass Spectrometry; D007220:Industrial Waste; D019906:Nuclear Magnetic Resonance, Biomolecular; D054813:Polyhydroxyalkanoates / Q000032:analysis / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
D007220:Industrial Waste; D054813:Polyhydroxyalkanoates
Biopolymers; FTIR; Fermentation; PHB; Pineapple