Polysaccharides from Arctium lappa L.: Chemical structure and biological activity.
The plant Arctium lappa L. is popularly used to relieve symptoms of inflammatory disorders. A crude polysaccharide fraction (SAA) resulting of aqueous extraction of A. lappa leaves showed a dose dependent anti-edematogenic activity on carrageenan-induced paw edema, which persisted for up to 48h. Sequential fractionation by ultrafiltration at 50kDa and 30kDa cut-off membranes yielded three fractions, namely RF50, RF30, and EF30. All these maintained the anti-edematogenic effect, but RF30 showed a more potent action, inhibiting 57% of the paw edema at a dose of 4.9mg/kg. The polysaccharide RF30 contained galacturonic acid, galactose, arabinose, rhamnose, glucose, and mannose in a 7:4:2:1:2:1 ratio and had a Mw of 91,000g/mol. Methylation analysis and NMR spectroscopy indicated that RF30 is mainly constituted by a type I rhamnogalacturonan branched by side chains of types I and II arabinogalactans, and arabinan.
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CEP 81531-980, CP 19046, Curitiba, PR, Brazil.
Juliane
J
Carlotto
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CEP 81531-980, CP 19046, Curitiba, PR, Brazil; Instituto de Pesquisa Pelé Pequeno Príncipe, Faculdades Pequeno Príncipe, CEP 80250-060, Curitiba, PR, Brazil.
Lauro M
LM
de Souza
Departamento de Farmacologia, Universidade Federal do Paraná, CEP 81531-980, Curitiba, PR, Brazil.
Cristiane H
CH
Baggio
Departamento de Farmacologia, Universidade Federal do Paraná, CEP 81531-980, Curitiba, PR, Brazil.
Maria Fernanda de P
MF
Werner
Departamento de Farmacologia, Universidade Federal do Paraná, CEP 81531-980, Curitiba, PR, Brazil.
Daniele
D
Maria-Ferreira
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CEP 81531-980, CP 19046, Curitiba, PR, Brazil.
Guilherme L
GL
Sassaki
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CEP 81531-980, CP 19046, Curitiba, PR, Brazil.
Marcello
M
Iacomini
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CEP 81531-980, CP 19046, Curitiba, PR, Brazil. Electronic address: trcipriani@ufpr.br.
Thales R
TR
Cipriani
D000818:Animals; D031170:Arctium / Q000737:chemistry*; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D005591:Chemical Fractionation; D002850:Chromatography, Gel; D004487:Edema / Q000188:drug therapy / Q000473:pathology; D005260:Female; D051379:Mice; D009005:Monosaccharides / Q000032:analysis; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology* / Q000627:therapeutic use; D066244:Proton Magnetic Resonance Spectroscopy
D009005:Monosaccharides; D011134:Polysaccharides
Anti-edematogenic activity; Arabinogalactan; Arctium lappa L.; Rhamnogalacturonan