Interactions of DNA and Proteins: Electrophoretic Mobility Shift Assay in Asthma.
Electrophoretic mobility shift assays (EMSA) are used to characterize interactions between nucleic acids and proteins in native conditions. This is based on the fact that the electrophoretic mobility of a nucleic acid becomes slower when it forms complexes with proteins. There are many different variants and applications of this methodology. In this chapter we describe a detailed EMSA protocol applied to the study of asthma.
Department of Clinical Genetics, University Hospital of Navarra, Pamplona, Navarra, Spain. virginia.garcia.solaesa@navarra.es.|Salamanca Institute for Biomedical Research (IBSAL), University of Salamanca, Salamanca, Spain. virginia.garcia.solaesa@navarra.es.
Virginia
V
García-Solaesa
Salamanca Institute for Biomedical Research (IBSAL), University of Salamanca, Salamanca, Spain.|Department of Microbiology and Cenetics, University of Salamanca, Salamanca, Spain.
Catalina S
CS
Sanz-Lozano
D001249:Asthma / Q000235:genetics* / Q000378:metabolism; D001665:Binding Sites; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay / Q000379:methods*; D006801:Humans; D011485:Protein Binding
D004268:DNA-Binding Proteins; D004247:DNA
Binding; EMSA; Electrophoretic mobility shift assay; Gel retardation; Protein–DNA complex