Resonance Rayleigh scattering method for highly sensitive detection of chitosan using aniline blue as probe.
This paper describes a highly sensitive and accurate approach using aniline blue (AB) (water soluble) as a probe to determine chitosan (CTS) through Resonance Rayleigh scattering (RRS). Under optimum experimental conditions, the intensities of RRS were linearly proportional to the concentration of CTS in the range from 0.01 to 3.5μg/mL, and the limit of detection (LOD) was 6.94ng/mL. Therefore, a new and highly sensitive method based on RRS for the determination of CTS has been developed. Furthermore, the effect of molecular weight of CTS and the effect of the degree of deacetylation of CTS on the accurate quantification of CTS was studied. The experimental data was analyzed by linear regression analysis, which indicated that the molecular weight and the degree of deacetylation of CTS had no statistical significance and this method could be used to determine CTS accurately. Meanwhile, this assay was applied for CTS determination in health products with satisfactory results.
School of Public Health, Guangdong Pharmaceutical University, Guangzhou, Guangdong Province 510310, China. Electronic address: zhangweiai0629@126.com.
Weiai
W
Zhang
School of Public Health, Guangdong Pharmaceutical University, Guangzhou, Guangdong Province 510310, China. Electronic address: mcjane2013@163.com.
Caijuan
C
Ma
School of Public Health, Guangdong Pharmaceutical University, Guangzhou, Guangdong Province 510310, China; Key Research Center of Liver Regulation for Hyperlipidemia SATCM/Class III Laboratory of Metabolism SATCM, Guangdong TCM Key Laboratory for Metabolic Diseases, Guangdong Pharmaceutical University, Guangzhou 510006, China. Electronic address: suzhq@scnu.edu.cn.
Zhengquan
Z
Su
School of Public Health, Guangdong Pharmaceutical University, Guangzhou, Guangdong Province 510310, China. Electronic address: angell_bai@163.com.
Yan
Y
Bai
D000107:Acetylation; D000814:Aniline Compounds / Q000737:chemistry*; D002021:Buffers; D048271:Chitosan / Q000032:analysis*; D004573:Electrolytes / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D008027:Light; D057230:Limit of Detection; D012542:Scattering, Radiation; D013696:Temperature
D000814:Aniline Compounds; D002021:Buffers; D004573:Electrolytes; D005456:Fluorescent Dyes; C017006:aniline blue; D048271:Chitosan
Aniline blue; Chitosan; Resonance Rayleigh scattering