Cerebral and extracerebral vulnerability to hypoxic insults after diffuse traumatic brain injury in rats.
The post-traumatic brain vulnerability suggests that after traumatic brain injury (TBI), the brain may be more susceptible to posttraumatic hypoxic insults. This concept could be extended to 'peripheral' organs, as non-neurologic organ failure is common after TBI. This study aims to characterize and quantify cerebral and extracerebral tissue hypoxia with pimonidazole resulting from a standardized hypoxia-hypotension (HH) phase occurring after a diffuse experimental TBI in rats. Rats were allocated to Sham (n=5), TBI (n=7), HH (n=7) and TBI+HH (n=7) groups. Then, pimonidazole was injected and brain, liver, heart and kidneys were analysed. In the cerebral cortex, post-treatment hypoxia was higher in TBI+HH group than Sham group (p=0.003), HH group (p=0.003) and TBI group (p=0.002). Large trends in thalamus, hippocampus and striatum for the TBI+HH group compared to the other groups were observed. For the heart and liver, the 4 groups were comparable. For the kidneys, post-treatment hypoxia was higher in the TBI group compared to the Sham and HH groups, but not more than TBI+HH group. This study reveals that a posttraumatic hypoxic insult occurring after a severe TBI has major hypoxic consequences on brain structures. However, TBI by itself appears to induce renal hypoxia that is not enhanced by posttraumatic hypoxic insult.
Equipe d'accueil' Modélisation de l'aggression tissulaire et nociceptive', University Toulouse 3 Paul Sabatier, Toulouse, France; Departement of Anesthesiology and Critical Care, University Hospital of Toulouse, Toulouse, France. Electronic address: mrozek.s@chu-toulouse.fr.
Ségolène
S
Mrozek
Equipe d'accueil' Modélisation de l'aggression tissulaire et nociceptive', University Toulouse 3 Paul Sabatier, Toulouse, France; Departement of Anesthesiology and Critical Care, University Hospital of Toulouse, Toulouse, France. Electronic address: aymericluzi@yahoo.com.
Aymeric
A
Luzi
Equipe d'accueil' Modélisation de l'aggression tissulaire et nociceptive', University Toulouse 3 Paul Sabatier, Toulouse, France; Departement of Anesthesiology and Critical Care, University Hospital of Toulouse, Toulouse, France. Electronic address: gonzalez.l@chu-toulouse.fr.
Leslie
L
Gonzalez
Equipe d'accueil' Modélisation de l'aggression tissulaire et nociceptive', University Toulouse 3 Paul Sabatier, Toulouse, France; Departement of Anesthesiology and Critical Care, University Hospital of Toulouse, Toulouse, France. Electronic address: lionel.kerhuel@gmail.com.
Lionel
L
Kerhuel
Equipe d'accueil' Modélisation de l'aggression tissulaire et nociceptive', University Toulouse 3 Paul Sabatier, Toulouse, France; Departement of Anesthesiology and Critical Care, University Hospital of Toulouse, Toulouse, France. Electronic address: fourcade.o@chu-toulouse.fr.
Olivier
O
Fourcade
Equipe d'accueil' Modélisation de l'aggression tissulaire et nociceptive', University Toulouse 3 Paul Sabatier, Toulouse, France; Departement of Anesthesiology and Critical Care, University Hospital of Toulouse, Toulouse, France. Electronic address: geeraerts.t@chu-toulouse.fr.
Thomas
T
Geeraerts
D000818:Animals; D062186:Arterial Pressure; D001921:Brain / Q000378:metabolism*; D000070642:Brain Injuries, Traumatic / Q000150:complications*; D004195:Disease Models, Animal; D007022:Hypotension / Q000209:etiology / Q000378:metabolism; D000860:Hypoxia / Q000150:complications / Q000378:metabolism*; D002534:Hypoxia, Brain / Q000209:etiology / Q000378:metabolism; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D009593:Nitroimidazoles / Q000008:administration & dosage; D051381:Rats; D017207:Rats, Sprague-Dawley
D009593:Nitroimidazoles; C033815:pimonidazole
Brain-organ crosstalk; Hypoxia-hypotension; Posttraumatic vulnerability; Tissue hypoxia