Flow cytometric enumeration of bacterial in the coral surface mucus layer.
The direct counts of bacteria inhabiting coral mucus were performed by flow cytometry testing four fluorescent dyes (SYBR®Green I, HCS, TOPRO®3, SYTO®62) with three different scleractinian species. Results obtained with SYTO62 were the most reliable based on the comparison with standardized epifluorescence counts and the resolution of cytograms.
UMR MARBEC (IRD, CNRS, UM, IFREMER) Marine Biodiversity, Exploitation and Conservation, Montpellier, France.
Yvan
Y
Bettarel
Institute of Biotechnology of Hanoi, VAST, Viet Nam.
Mai Chi
MC
Thanh
UMR MARBEC (IRD, CNRS, UM, IFREMER) Marine Biodiversity, Exploitation and Conservation, Montpellier, France.
Got
G
Patrice
Centre de Recherche Océanologique d'Abidjan, Cote d'Ivoire.
Adingra
A
Antoinette
Centre de Recherche Océanologique d'Abidjan, Cote d'Ivoire.
Kouadio-Ngbesso
KN
Nadège
Institute of Biotechnology of Hanoi, VAST, Viet Nam.
Van Ngoc
VN
Bui
UMR MARBEC (IRD, CNRS, UM, IFREMER) Marine Biodiversity, Exploitation and Conservation, Montpellier, France.
Bouvier
B
Thierry
D000818:Animals; D037421:Anthozoa / Q000382:microbiology*; D001419:Bacteria / Q000302:isolation & purification*; D015169:Colony Count, Microbial; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D050296:Microbial Viability
D005456:Fluorescent Dyes
Bacteria; Corals; Epibionts; Fluorescent dyes; Microscopy; Mucus