Endogenous nitric oxide mediates He-Ne laser-induced adaptive responses in salt stressed-tall fescue leaves.
The aim of this study was to investigate the role of endogenous nitric oxide in protective effects of He-Ne laser on salt stressed-tall fescue leaves. Salt stress resulted in significant increases of membrane injury, reactive oxygen species (ROS) production, polyamine accumulation, and activities of SOD, POD, and APX, while pronounced decreases of antioxidant contents, CAT activity and intracellular Ca(2+) concentration in seedlings leaves. He-Ne laser illumination caused a distinct alleviation of cellular injury that was reflected by the lower MDA amounts, polyamine accumulation and ROS levels at the stress period. In contrast, the laser treatment displayed a higher Ca(2+) concentration, antioxidant amounts, NO release, antioxidant enzyme, and NOS activities. These responses could be blocked due to the inhibition of NO biosynthesis by PTIO (NO scavenger) or LNNA (NOS inhibitor). The presented results demonstrated that endogenous NO might be involved in the progress of He-Ne laser-induced plant antioxidant system activation and ROS degradation in order to enhance adaptive responses of tall fescue to prolonged saline conditions.
a Higher Education Key Laboratory of Plant Molecular and Environmental Stress Response , Shanxi Normal University , Linfen , PR China.|b Analysis and Testing Center , Shanxi Normal University , Linfen , PR China.
Yongfeng
Y
Li
a Higher Education Key Laboratory of Plant Molecular and Environmental Stress Response , Shanxi Normal University , Linfen , PR China.|c College of Life Science , Shanxi Normal University , Linfen , PR China.
Limei
L
Gao
a Higher Education Key Laboratory of Plant Molecular and Environmental Stress Response , Shanxi Normal University , Linfen , PR China.|c College of Life Science , Shanxi Normal University , Linfen , PR China.
Rong
R
Han
D000222:Adaptation, Physiological / Q000187:drug effects / Q000528:radiation effects*; D000975:Antioxidants / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D031751:Festuca / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology / Q000528:radiation effects; D006861:Hydrogen Peroxide / Q000378:metabolism; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D054020:Lasers, Gas; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology / Q000528:radiation effects; D011073:Polyamines / Q000378:metabolism; D012492:Salts / Q000494:pharmacology*; D036226:Seedlings / Q000187:drug effects / Q000378:metabolism / Q000502:physiology / Q000528:radiation effects; D013312:Stress, Physiological / Q000187:drug effects* / Q000528:radiation effects; D013481:Superoxides / Q000378:metabolism
D000975:Antioxidants; D011073:Polyamines; D012492:Salts; D013481:Superoxides; D009569:Nitric Oxide; D006861:Hydrogen Peroxide; D019001:Nitric Oxide Synthase; D002118:Calcium
He–Ne laser; ROS level; nitric oxide; plant antioxidant system; polyamine