[Effect of Taohong Qinlian Decoction on HMGB1 in Septic Rat Cardiac Muscle].
OBJECTIVE
To observe the levels of high mobility group box-1 protein (HMGB1), tumor necrosis factor-alpha (TNF-α), IL-6, troponin I (Tn I) release in septic rats, and to explore themechanism of Taohong Qinlian Decoction (TQD) in the treatment of septic myocardial injury.


METHODS
A total of 48 healthy male Wistar rats of clean grade were randomly divided into the sham-operation group (Sham), the sepsis model group (CLP), and the TQD treatment group (ZY), 16 in each group. Concen-trations of TNF-α, IL-6, Tn I, and HMGB1 expression were detected in each group at 24 and 48 h after operation. Pathological changes of cardiac muscle were observed under light microscope.


RESULTS
Concentrations of TNF-α, IL-6, Tn I and HMGB1 at 24 and 48 h after operation were significantly higher in the CLP group than in the Sham group (P < 0.01). Concentrations of TNF-α, IL-6, Tn I, and HMGB1 at 24 and 48 h after operation were significantly lower in the ZY group than in the CLP group (P < 0.05). Myocardial injury occurred in the CLP and the ZY group under light microscope. And this injury was more severe in the CLP group than in the ZY group.


CONCLUSIONS
TQL could reduce the level of sepsis-related inflammatory cytokines and protect myocardium in septic rats.
Zhong-min
ZM
Gu
Shao-bin
SB
Lin
Cai-jun
CJ
Liu
Xin-xin
XX
Qi
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D024243:HMGB1 Protein / Q000378:metabolism*; D006321:Heart / Q000187:drug effects; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000473:pathology*; D019210:Troponin I / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D024243:HMGB1 Protein; C104200:Hbp1 protein, rat; D015850:Interleukin-6; D019210:Troponin I; D014409:Tumor Necrosis Factor-alpha