Detection of Methylated DNA on a Power-Free Microfluidic Chip with Laminar Flow-Assisted Dendritic Amplification.
We report on a detection method for methylated DNA on a microfluidic chip, which needs no external power for fluid pumping. The methylated DNA was sandwiched by immobilized probe DNA and an anti-methylcytosine antibody. The fluorescence signal was amplified by our original amplification technology. The detection method was first optimized using a 22-mer DNA sequence, then further validated using a 60-mer DNA sequence adapted from the SEPT9 gene. We were able to detect the methylated 60-mer DNA at 0.4 nM within 18 min.
Bioengineering Laboratory, RIKEN.
Kazuki
K
Hasegawa
Mutsuyoshi
M
Matsumoto
Kazuo
K
Hosokawa
Mizuo
M
Maeda
D044503:5-Methylcytosine / Q000378:metabolism; D001483:Base Sequence; D015374:Biosensing Techniques / Q000295:instrumentation*; D002138:Calibration; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000235:genetics*; D019175:DNA Methylation; D015342:DNA Probes / Q000737:chemistry; D006801:Humans; D056656:Lab-On-A-Chip Devices; D057230:Limit of Detection; D058112:Septins / Q000235:genetics
D015342:DNA Probes; D044503:5-Methylcytosine; D004247:DNA; C474315:SEPTIN9 protein, human; D058112:Septins