A large stokes-shifted fluorescent dye synthesized as a new probe for the determination of protein.
A novel fluorescent dye, 1-(2-hydroxyethyl)-4-((E)-2-(3-benzothiazol-2yl-9-ethyl-carbazole-3yl)vinyl) pyridinium bromide, was synthesized for determination of protein and its structure was characterized by (1)H NMR. Photophysics of the new probe in different solvents has been delineated in this paper, the new fluorescent molecular dye exhibited a large stokes-shifted and fluorescence quantum yields in organic solvent. The photostability and thermostability of the new dye were also studied and the results suggested the stable was excellent. The interactions of the dye with bovine serum albumin (BSA) , Human serumal bumin (HSA) and calf thymus deoxyribonucleic acid (ctDNA) were studied by fluorescence and absorption spectroscopy. The binding constant for BSA, HSA and DNA were calculated to be 8.91 × 10(7), 1.86 × 10(6) and 2.9 × 10(4), respectively. The experimental results indicated a potential value of the new dye for biomarker.
School of Envirnmental Science and Engineering, Tianjin University, 92 Weijin Road, Nankai District, Tianjin, 300072, China.
Dayong
D
Lin
School of Envirnmental Science and Engineering, Tianjin University, 92 Weijin Road, Nankai District, Tianjin, 300072, China. xueningfei@126.com.|School of Science, Tianjin Chengjian University, No.26 Jinjing Road, Xiqing District, Tianjin, 300384, China. xueningfei@126.com.
Xuening
X
Fei
School of Science, Tianjin Chengjian University, No.26 Jinjing Road, Xiqing District, Tianjin, 300384, China.
Ran
R
Li
School of Science, Tianjin Chengjian University, No.26 Jinjing Road, Xiqing District, Tianjin, 300384, China. jvgugugu@126.com.|Institute of Chemistry, Chinese Academy of Sciences (ICCAS), Zhongguancun North First Street 2, Beijing, 100190, China. jvgugugu@126.com.
Yingchun
Y
Gu
Institute of Chemistry, Chinese Academy of Sciences (ICCAS), Zhongguancun North First Street 2, Beijing, 100190, China. tangyl@iccas.ac.cn.
Yalin
Y
Tang
School of Science, Tianjin Chengjian University, No.26 Jinjing Road, Xiqing District, Tianjin, 300384, China.
Jianguo
J
Zhou
School of Science, Tianjin Chengjian University, No.26 Jinjing Road, Xiqing District, Tianjin, 300384, China.
Baolian
B
Zhang
D000818:Animals; D052160:Benzothiazoles / Q000138:chemical synthesis* / Q000737:chemistry*; D002417:Cattle; D060326:Chemistry Techniques, Synthetic; D004247:DNA / Q000032:analysis / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry; D006801:Humans; D011726:Pyridinium Compounds / Q000138:chemical synthesis* / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000032:analysis* / Q000737:chemistry*; D000075462:Serum Albumin, Human / Q000032:analysis* / Q000737:chemistry*; D013696:Temperature
C000626306:1-(2-hydroxyethyl)-4-((E)-2-(3-benzothiazol-2yl-9-ethyl-carbazole-3yl)vinyl)pyridinium bromide; D052160:Benzothiazoles; D005456:Fluorescent Dyes; D011726:Pyridinium Compounds; D012710:Serum Albumin, Bovine; D004247:DNA; D000075462:Serum Albumin, Human
Fluorescent probe; Protein; Specific; Spectra properties