Progress in the development of lipopolyplexes as efficient non-viral gene delivery systems.
Efficient gene therapy is mainly dependent on the gene transfer capability of gene delivery vectors. Non-viral vectors have become the research interest of many researchers because these vectors are safer than viral vectors. Acquiring the advantages of both polyplexes and lipoplexes, the lipopolyplex (LPP) is a ternary nanocomplex composed of cationic liposome, polycation, and nucleic acid. Considering the polycationic component, ternary complexes (LPPs) are divided into cationic polymer-based LPPs and cationic peptide-based LPPs. Considering the capability of rational design, LPP is an interesting field of research to design a more potent nucleic acid carrier. With the promising transfection activity and safety observed in the LPPs, many researchers have formulated various types of lipids and polycations to achieve an efficient and safe carrier for gene therapy. Here we provide a review on the designed LPPs for efficient delivery of different nucleic acids such as plasmid DNA, siRNA, shRNA, and DNA vaccines.
Department of Medical Biotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: RezaeiM911@mums.ac.ir.
Mehdi
M
Rezaee
Targeted Drug Delivery Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: OskueeKR@mums.ac.ir.
Reza Kazemi
RK
Oskuee
Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: Nasirleeh1@mums.ac.ir.
Hooriyeh
H
Nassirli
Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: MalaekehB@mums.ac.ir.
Bizhan
B
Malaekeh-Nikouei
D000818:Animals; D002626:Chemistry, Pharmaceutical; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D006801:Humans; D008055:Lipids / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D009696:Nucleic Acids / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D014162:Transfection
D008055:Lipids; D008081:Liposomes; D009696:Nucleic Acids; D010455:Peptides; D011108:Polymers
CHEMS, cholesteryl hemisuccinate (PubChem CID: 65082);; Chol, cholesterol (PubChem CID: 5997);; DDAB, dimethyldioctadecylammonium bromide (PubChem CID: 77293);; DMPE, dimyristoylphosphatidylethanolamine (PubChem CID: 114944);; DMPG, 1,2-ditetradecanoyl-sn-glycero-3-phospho-1′-rac-glycerol sodium salt (PubChem CID: 71311889);; DODAC, dioleyldimethylammonium chloride (PubChem CID: 6436349);; DOPC, 1,2-dioleoyl-sn-glycero-3-phosphocholine (PubChem CID: 10350317);; DOPE, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (PubChem CID: 44251425);; DOTAP, 1,2-dioleoyl-3-trimethylammonium-propane (PubChem CID: 107898);; DOTMA, N-[1-(2,3-dioleyloxy) propyl]-N,N,N-trimethlylammonium chloride (PubChem CID: 6438350);; DPPE, 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (PubChem CID: 46907860);; DPTAP, 1,2-dipalmitoyl-3-trimethylammonium-propane (PubChem CID: 107898);; DTDTMA, ditetradecyl trimethyl ammonium (PubChem CID: 14251);; Gene delivery; Gene therapy; HZN, pyridylhydrazone (PubChem CID: 6436327);; IL-2 and 12, interleukin-2 and 12 (PubChem CID: 130138);; Lipopolyplexes; Non-viral vectors; PEG, poly(ethylene glycol) (PubChem CID: 174);; Rhod-DOPE, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) ammonium salt (PubChem CID: 44251425)