Review of Methods to Study Gene Expression Regulation Applied to Asthma.
Gene expression regulation is the cellular process that controls, increasing or decreasing, the expression of gene products (RNA or protein). A complex set of interactions between genes, RNA molecules, protein, and other components determined when and where specific genes are activated and the amount of protein or RNA produced. Here, we focus on several methods to study gene regulation applied to asthma and allergic research such as: Western Blot to identify and quantify proteins, electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation (ChIP) to study protein interactions with nucleic acids, and RNA interference (RNAi) by which gene expression could be silenced.
Department of Biomedical and Diagnostic Sciences, University of Salamanca, Salamanca, Spain. chonela@usal.es.|Salamanca Institute for Biomedical Research (IBSAL), UniversityHospital of Salamanca, Salamanca, Spain. chonela@usal.es.
Asunción
A
García-Sánchez
Salamanca Institute for Biomedical Research (IBSAL), Salamanca, Spain.|Department of Clinical Biochemistry, University Hospital of Salamanca, Salamanca, Spain.
Fernando
F
Marqués-García
D001249:Asthma / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D047369:Chromatin Immunoprecipitation; D024202:Electrophoretic Mobility Shift Assay; D015870:Gene Expression; D005786:Gene Expression Regulation; D006801:Humans; D011506:Proteins / Q000235:genetics* / Q000378:metabolism*
D011506:Proteins
Allergy; Asthma; Chromatin immunoprecipitation (ChIP); DNA–protein; Electrophoretic mobility shift assay (EMSA); Gene regulation; Western blot; siRNA