Effect of growth temperature on glucosinolate profiles in Arabidopsis thaliana accessions.
Glucosinolates are plant secondary metabolites with important roles in plant defence against pathogens and pests and are also known for their health benefits. Understanding how environmental factors affect the level and composition of glucosinolates is therefore of importance in the perspective of climate change. In this study we analysed glucosinolates in Arabidopsis thaliana accessions when grown at constant standard (21 °C), moderate (15 °C) and low (9 °C) temperatures during three generations. In most of the tested accessions moderate and pronounced chilling temperatures led to higher levels of glucosinolates, especially aliphatic glucosinolates. Which temperature yielded the highest glucosinolate levels was accession-dependent. Transcriptional profiling revealed also accession-specific gene responses, but only a limited correlation between changes in glucosinolate-related gene expression and glucosinolate levels. Different growth temperatures in one generation did not consistently affect glucosinolate composition in subsequent generations, hence a clear transgenerational effect of temperature on glucosinolates was not observed.
Department of Biology, Norwegian University of Science and Technology, Høgskoleringen 5, NO-7491, Trondheim, Norway.
Ralph
R
Kissen
Department of Biology, Norwegian University of Science and Technology, Høgskoleringen 5, NO-7491, Trondheim, Norway.
Franziska
F
Eberl
Department of Biology, Norwegian University of Science and Technology, Høgskoleringen 5, NO-7491, Trondheim, Norway.
Per
P
Winge
NIBIO, Norwegian Institute of Bioeconomy Research, Box 115, NO-1431, Ås, Norway.
Eivind
E
Uleberg
NIBIO, Norwegian Institute of Bioeconomy Research, Box 115, NO-1431, Ås, Norway.
Inger
I
Martinussen
Department of Biology, Norwegian University of Science and Technology, Høgskoleringen 5, NO-7491, Trondheim, Norway. Electronic address: atle.m.bones@ntnu.no.
Atle M
AM
Bones
D017360:Arabidopsis / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000378:metabolism; D057231:Climate Change; D058639:Cold-Shock Response; D018506:Gene Expression Regulation, Plant; D005961:Glucosinolates / Q000032:analysis / Q000378:metabolism* / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D013696:Temperature
D029681:Arabidopsis Proteins; D005961:Glucosinolates
Abiotic stress; Arabidopsis thaliana; Brassicaceae; Chilling; Epigenetics; Glucosinolates; Parental effect; Secondary metabolites; Temperature; Transgenerational effect