Ultrasound-Assisted Extraction of Stilbenes from Grape Canes.
An analytical ultrasound-assisted extraction (UAE) method has been optimized and validated for the rapid extraction of stilbenes from grape canes. The influence of sample pre-treatment (oven or freeze-drying) and several extraction variables (solvent, sample-solvent ratio and extraction time between others) on the extraction process were analyzed. The new method allowed the main stilbenes in grape canes to be extracted in just 10 min, with an extraction temperature of 75 °C and 60% ethanol in water as the extraction solvent. Validation of the extraction method was based on analytical properties. The resulting RSDs (n = 5) for interday/intraday precision were less than 10%. Furthermore, the method was successfully applied in the analysis of 20 different grape cane samples. The result showed that grape cane byproducts are potentially sources of bioactive compounds of interest for pharmaceutical and food industries.
IFAPA Rancho de la Merced, Carretera de Trebujena, Km. 2.2, Apdo. 589, Jerez de la Frontera 11471, Spain. zulema.pineiro@juntadeandalucia.es.
Zulema
Z
Piñeiro
IFAPA Rancho de la Merced, Carretera de Trebujena, Km. 2.2, Apdo. 589, Jerez de la Frontera 11471, Spain. almudena.marrucurti@mail.uca.es.
Almudena
A
Marrufo-Curtido
IFAPA Rancho de la Merced, Carretera de Trebujena, Km. 2.2, Apdo. 589, Jerez de la Frontera 11471, Spain. mariajose.serrano@uca.es.
Maria Jose
MJ
Serrano
Departamento de Qu&#237;mica Anal&#237;tica, IVAGRO, Universidad de Cadiz, Apdo. 40, Puerto Real 11510, Spain. miguel.palma@uca.es.
Miguel
M
Palma
D002851:Chromatography, High Pressure Liquid; D019220:High-Energy Shock Waves; D010936:Plant Extracts / Q000737:chemistry*; D018547:Plant Stems / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013267:Stilbenes / Q000737:chemistry / Q000302:isolation & purification*; D014465:Ultrasonics; D027843:Vitis / Q000737:chemistry*
D010936:Plant Extracts; D012997:Solvents; D013267:Stilbenes
HPLC; extraction; grape canes; stilbenes; trans-resveratrol; ultrasound