5,6-Epoxidation of All-trans-retinoic Acid with Soybean Lipoxygenase-2 and -3.
Cooxidation activity of highly purified soybean lipoxygenase-2 and -3 was investigated with all-trans-retinoic acid as a co-substrate. Both the isoenzymes rapidly degraded retinoic acid in the presence of linoleic acid, but lipoxygenase-2 had more cooxidation activity than lipoxygenase-3. During cooxidation, 5, 6-epoxyretinoic acid was formed as a major product. HPLC on a chiral phase (Ceramospher Chiral RU-1) found a racemic mixture of the epoxide, indicating its formation by oxidative attack by peroxyl radicals.
a Department of Biological Chemistry , Faculty of Agriculture, Yamaguchi University , Yamaguchi 753 , Japan.
K
K
Matsui
a Department of Biological Chemistry , Faculty of Agriculture, Yamaguchi University , Yamaguchi 753 , Japan.
T
T
Kajiwara
a Department of Biological Chemistry , Faculty of Agriculture, Yamaguchi University , Yamaguchi 753 , Japan.
A
A
Hatanaka
a Department of Biological Chemistry , Faculty of Agriculture, Yamaguchi University , Yamaguchi 753 , Japan.|b Lehrstuhl für Lebensmittelchemie, Universität Würzburg , Am Hubland , 97074 Würzburg , Germany.
D
D
Waldmann
a Department of Biological Chemistry , Faculty of Agriculture, Yamaguchi University , Yamaguchi 753 , Japan.|b Lehrstuhl für Lebensmittelchemie, Universität Würzburg , Am Hubland , 97074 Würzburg , Germany.
P
P
Schreier