Effects of silver nanoparticles on the freshwater snail Physa acuta: The role of test media and snails' life cycle stage.
Silver nanoparticles (AgNPs) are widely used worldwide, most likely leading to their release into the environment and a subsequent increase of environmental concentrations. Studies of their deleterious effects on organisms is crucial to understand their environmental impacts. The freshwater snail Physa acuta was chosen to evaluate the potential deleterious effects of AgNPs and their counterpart AgNO3, through water-only exposures. The toxicity of AgNPs is greatly influenced by medium composition. Thus, 2 media were tested: artificial pond water (APW) and modified APW (adapted by removing calcium chloride). Acute tests (96 h) were performed with juvenile and adult snails in both media to assess lethality, and egg mass chronic tests were conducted with APW medium only to assess embryo viability and mortality, carried out until 90% hatching success was reached in the control. Acute toxicity increased with decreasing shell length for both silver forms (ion and nanoparticle); that is, juveniles were more sensitive than adults. Different test media led to dissimilar median lethal concentrations, with chloride playing an important role in toxicity, most likely by complexation with silver ions, which would reduce the bioavailability, uptake, and toxicity of silver. Chronic tests showed that hatching success was more sensitive to silver in the ionic form than in the particulate form. Different forms of silver, exposure media, and life cycle stages led to different patterns of toxicity, highlighting an impairment in the snails' life cycle. Environ Toxicol Chem 2017;36:243-253. © 2016 SETAC.
Department of Biology and Center for Environmental and Marine Studies (CESAM), University of Aveiro, Aveiro, Portugal.
Sandra
S
F Gonçalves
Department of Biology and Center for Environmental and Marine Studies (CESAM), University of Aveiro, Aveiro, Portugal.
Maria
M
D Pavlaki
Department of Biology and Center for Environmental and Marine Studies (CESAM), University of Aveiro, Aveiro, Portugal.
Rafael
R
Lopes
Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
Julia
J
Hammes
Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
Julián Alberto
JA
Gallego-Urrea
Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
Martin
M
Hassellöv
Department of Materials, Oxford University Begbroke Science Park, Begbroke, United Kingdom.
Kerstin
K
Jurkschat
Department of Materials, Oxford University Begbroke Science Park, Begbroke, United Kingdom.
Alison
A
Crossley
Department of Biology and Center for Environmental and Marine Studies (CESAM), University of Aveiro, Aveiro, Portugal.
Susana
S
Loureiro
D000818:Animals; D005618:Fresh Water / Q000737:chemistry*; D008018:Life Cycle Stages / Q000187:drug effects*; D053768:Metal Nanoparticles / Q000633:toxicity*; D012834:Silver / Q000633:toxicity*; D012908:Snails / Q000187:drug effects* / Q000254:growth & development; D023382:Toxicity Tests, Acute; D036401:Toxicity Tests, Chronic; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D014874:Water Pollutants, Chemical; D012834:Silver
Chloride; Life cycle; Physa acuta; Silver ions; Silver nanoparticles; Test media