Toxic effects of imidacloprid on adult loach (Misgurnus anguillicaudatus).
The present investigation was aimed to assess the effects of imidacloprid on the survival, genetic materials, hepatic transaminase activity and histopathology of loach (Misgurnus anguillicaudatus). The values of LC50 (24, 48, 72 and 96h) of imidacloprid were 167.7, 158.6, 147.9 and 145.8mg/L, respectively, and the safety concentration was 42.55mg/L. The erythrocyte micronuclei assays and the comet assay results showed that imidacloprid had genetic toxic effect on the loach erythrocytes. To assess the physiological and biochemical damage caused by imidacloprid, the activities of hepatic glutamic-pyruvic transaminase (GPT) and glutamic-oxalacetic transaminase (GOT) were measured and their values declined in treatment groups. Histological examination of testis revealed that imidacloprid treatment resulted in disorganized lobules and cysts structures. In the present work, we also investigated the joint toxicity of pesticides commonly used in paddy fields (imidacloprid and lambda-cyhalothrin) on M. anguillicaudatus, and confirmed that a synergistic effect existing in the binary mixtures. The results of our study provide relevant and comparable toxicity information that are useful for safety application of pesticides.
College of Life Science, Henan Normal University, Xinxiang, Henan 453007, People's Republic of China.
Xiaohua
X
Xia
College of Life Science, Henan Normal University, Xinxiang, Henan 453007, People's Republic of China.
Xiaopei
X
Xia
College of Life Science, Henan Normal University, Xinxiang, Henan 453007, People's Republic of China.
Weiran
W
Huo
College of Life Science, Henan Normal University, Xinxiang, Henan 453007, People's Republic of China.
Hui
H
Dong
College of Life Science, Henan Normal University, Xinxiang, Henan 453007, People's Republic of China.
Linxia
L
Zhang
College of Life Science, Henan Normal University, Xinxiang, Henan 453007, People's Republic of China. Electronic address: changzhongjie@tom.com.
Zhongjie
Z
Chang
D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D020552:Comet Assay; D003531:Cypriniformes / Q000235:genetics / Q000378:metabolism; D004357:Drug Synergism; D007093:Imidazoles / Q000633:toxicity*; D007306:Insecticides / Q000633:toxicity*; D007928:Lethal Dose 50; D008099:Liver / Q000033:anatomy & histology / Q000187:drug effects / Q000201:enzymology; D008297:Male; D015162:Micronucleus Tests; D000073943:Neonicotinoids; D009570:Nitriles / Q000633:toxicity*; D009574:Nitro Compounds / Q000633:toxicity*; D011722:Pyrethrins / Q000633:toxicity*; D013737:Testis / Q000187:drug effects / Q000473:pathology; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D007093:Imidazoles; D007306:Insecticides; D000073943:Neonicotinoids; D009570:Nitriles; D009574:Nitro Compounds; D011722:Pyrethrins; D014874:Water Pollutants, Chemical; C082359:imidacloprid; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; C037304:cyhalothrin
Comet assay; Imidacloprid; Joint toxicity; Misgurnus anguillicaudatus; Toxicity testing