Nuclear factor erythroid 2-related factor 2 antibody attenuates thermal hyperalgesia in the dorsal root ganglion: Neurochemical changes and behavioral studies after sciatic nerve-pinch injury.
Oxidative stress is generated in several peripheral nerve injury models.Nuclear factor erythroid 2-related factor 2 (Nrf2) is activated to have a role in antioxidant effect. After nerve injury, the severely painful behavior is also performed. However, little has been explored regarding the function of Nrf2 in this painful process. Therefore, in this study, we compared the effects of Nrf2 antibody administration following sciatic nerve-pinch injury on painful behavior induced in young mice and neurochemical changes in dorsal root ganglion neurons. After pinch nerve injury, we found that the magnitude of the thermal allodynia was significantly decreased after application of Nrf2 antibody (5ul, 1mg/ml) in such injured animals and phosphorylated ERK(p-ERK) as well as the apoptotic protein (i.e., Bcl-6) in DRG neurons were also down-regulated in the anti-Nrf2-treated injured groups compared to the saline-treated groups. Taken collectively, these data suggested that the Nrf2 antibody reduced thermal hyperalgesia via ERK pathway and the down regulation of Bcl-6 protein from the apoptosis pathway might be protecting against the protein deletions caused by anti-Nrf2 effect and suggested the new therapeutic strategy with Nrf2 inhibitor following nerve injury.
Institute of Medicine, Medical Research Center, Jishou University, Hunan, 416000, China.
Qiong
Q
Xiang
Institute of Medicine, Medical Research Center, Jishou University, Hunan, 416000, China.
Chao
C
Yu
Institute of Medicine, Medical Research Center, Jishou University, Hunan, 416000, China.
Yao-Feng
YF
Zhu
Institute of Medicine, Medical Research Center, Jishou University, Hunan, 416000, China.
Chun-Yan
CY
Li
Institute of Medicine, Medical Research Center, Jishou University, Hunan, 416000, China.
Rong-Bo
RB
Tian
Institute of Medicine, Medical Research Center, Jishou University, Hunan, 416000, China. Electronic address: lxh_surgeon@yeah.net.
Xian-Hui
XH
Li
D000818:Animals; D001522:Behavior, Animal / Q000502:physiology*; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D004195:Disease Models, Animal; D005727:Ganglia, Spinal / Q000378:metabolism*; D006930:Hyperalgesia / Q000378:metabolism*; D008297:Male; D051379:Mice; D051267:NF-E2-Related Factor 2 / Q000378:metabolism*; D010146:Pain / Q000378:metabolism*; D010147:Pain Measurement; D012584:Sciatic Nerve / Q000293:injuries*; D020426:Sciatic Neuropathy / Q000473:pathology*
D015415:Biomarkers; D051267:NF-E2-Related Factor 2; C495636:Nfe2l2 protein, mouse
DRG; Nerve injury; Nrf2; Oxidative stress; Thermal hyperalgesia