Potential Involvement of P2 Receptors in the Pathological Processes of Hyperthyroidism: A Pilot Study.
Symptoms of hyperthyroidism manifest mainly as changes in the nervous and metabolic systems. Whether P2X receptors (ionotropic ATP purinergic receptors, including P2X3 receptor and P2X7 receptor) are involved in the alterations of these disorders still remains unclear. Thus, this study aimed to assess the association of hyperthyroidism with the expression of P2X3 and P2X7 receptors and the concentrations of ATP in blood leukocytes and catecholamine. Twelve healthy subjects and twelve patients diagnosed with hyperthyroidism were recruited. Serum free triiodothyronine (FT3), free thyroxine (FT4) and thyroid stimulating hormone (TSH) levels had been detected by chemiluminescence method. Meanwhile, the catecholamine levels (including adrenaline, noradrenaline, and dopamine) in plasma, ATP level and P2X receptors (including P2X3 receptor and P2X7 receptor) in peripheral blood had been detected by high performance liquid chromatography, bioluminescence method, and reverse transcription polymerase chain reaction, respectively. Levels of epinephrine and norepinephrine were significantly higher in the hyperthyroidism group compared with the control group. The concentration of ATP in the hyperthyroidism group was significantly higher than its in the control group. The expression of P2X3 mRNA and P2X7 mRNA in hyperthyroidism group were significantly increased compared with those in control group. In a conclusion, there is a relationship between the elevated expression of P2X3 receptor and P2X7 receptor in peripheral blood leukocytes and high serum epinephrine and norepinephrine levels in hyperthyroidism patients.
School of Life Sciences, Nanchang University, Nanchang, Jiangxi, People's Republic of China Jiangxi Province Blood Center, Nanchang, Jiangxi, People's Republic of China.
Wu
W
Hong
Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi, People's Republic of China Department of Clinical Translational Research, Singapore General Hospital, Singapore.
Guodong
G
Li
First Affiliated Hospital, Medical School of Nanchang University, Nanchang, Jiangxi, People's Republic of China.
Yijun
Y
Nie
Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi, People's Republic of China.
Lifang
L
Zou
Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi, People's Republic of China.
Xi
X
Zhang
Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi, People's Republic of China.
Shuangmei
S
Liu
Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi, People's Republic of China.
Guilin
G
Li
Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi, People's Republic of China.
Hong
H
Xu
Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi, People's Republic of China.
Chun-Ping
CP
Zhang
Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi, People's Republic of China liangsd@hotmail.com.
Shangdong
S
Liang
D000255:Adenosine Triphosphate / Q000097:blood; D000328:Adult; D002395:Catecholamines / Q000097:blood; D003710:Demography; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D006980:Hyperthyroidism / Q000378:metabolism* / Q000473:pathology*; D007962:Leukocytes / Q000378:metabolism; D008297:Male; D010865:Pilot Projects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D058477:Receptors, Purinergic P2X3 / Q000235:genetics / Q000378:metabolism*; D058486:Receptors, Purinergic P2X7 / Q000235:genetics / Q000378:metabolism*
D002395:Catecholamines; D012333:RNA, Messenger; D058477:Receptors, Purinergic P2X3; D058486:Receptors, Purinergic P2X7; D000255:Adenosine Triphosphate
ATP; P2X3 receptor; P2X7 receptor; catecholamines hormones; hyperthyroidism