Collaborative Study of an Indirect Enzymatic Method for the Simultaneous Analysis of 3-MCPD, 2-MCPD, and Glycidyl Esters in Edible Oils.
A collaborative study was conducted to evaluate an indirect enzymatic method for the analysis of fatty acid esters of 3-monochloro-1,2-propanediol (3-MCPD), 2-monochloro-1,3-propanediol (2-MCPD), and glycidol (Gly) in edible oils and fats. The method is characterized by the use of Candida rugosa lipase, which hydrolyzes the esters at room temperature in 30 min. Hydrolysis and bromination steps convert esters of 3-MCPD, 2-MCPD, and glycidol to free 3-MCPD, 2-MCPD, and 3-monobromo-1,2-propanediol, respectively, which are then derivatized with phenylboronic acid, and analyzed by gas chromatography-mass spectrometry. In a collaborative study involving 13 laboratories, liquid palm, solid palm, rapeseed, and rice bran oils spiked with 0.5-4.4 mg/kg of esters of 3-MCPD, 2-MCPD, and Gly were analyzed in duplicate. The repeatability (RSDr) were < 5% for five liquid oil samples and 8% for a solid oil sample. The reproducibility (RSDR) ranged from 5% to 18% for all oil samples. These RSDR values were considered satisfactory because the Horwitz ratios were ≤ 1.3% for all three analytes in all oil samples. This method is applicable to the quantification of 3-MCPD, 2-MCPD, and Gly esters in edible oils.
Central Research & Development Institute, House Foods Group Inc.
Kazuo
K
Koyama
Kinuko
K
Miyazaki
Kousuke
K
Abe
Yoshitsugu
Y
Egawa
Hirotsugu
H
Kido
Tadashi
T
Kitta
Takashi
T
Miyashita
Toru
T
Nezu
Hidenori
H
Nohara
Takashi
T
Sano
Yukinari
Y
Takahashi
Hideji
H
Taniguchi
Hiroshi
H
Yada
Kumiko
K
Yamazaki
Yomi
Y
Watanabe
D002175:Candida / Q000201:enzymology; D004852:Epoxy Compounds / Q000032:analysis* / Q000378:metabolism; D005990:Glycerol / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D008049:Lipase / Q000378:metabolism*; D010938:Plant Oils / Q000737:chemistry*; D010945:Plants, Edible / Q000737:chemistry*; D000517:alpha-Chlorohydrin / Q000032:analysis* / Q000378:metabolism; D011409:Propylene Glycols
D004852:Epoxy Compounds; D005990:Glycerol; D008049:Lipase; D010938:Plant Oils; D000517:alpha-Chlorohydrin; C020344:glycidyl ethers; C585004:monochloropropanediol; D011409:Propylene Glycols