Protocol for Lipid-Mediated Transient Transfection in A549 Epithelial Lung Cell Line.
Trials of transfection in eukaryotic cells are essential tools for the study of gene and protein function. They have been used in a wide range of research fields. In this chapter, a method of transient transfection of the A549 cell line, human lung cells of alveolar epithelium, with an expression plasmid is described. In addition, the fundamental characteristics of this experimental procedure are addressed.
Department of Clinical Biochemistry, University Hospital of Salmanca, Paseo de San Vicente 58, Salmanca, 37007, Spain. elemarcos@hotmail.com.|Salamanca Institute for Biomedical Research (IBSAL), Salamanca, Spain. elemarcos@hotmail.com.
Elena
E
Marcos-Vadillo
Salamanca Institute for Biomedical Research (IBSAL), Salamanca, Spain.|Department of Biomedical and Diagnostic Sciences, University of Salamanca, Salamanca, Spain.
Asunción
A
García-Sánchez
D000072283:A549 Cells; D049109:Cell Proliferation; D002470:Cell Survival; D004847:Epithelial Cells / Q000166:cytology; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D008168:Lung / Q000166:cytology*; D010957:Plasmids / Q000235:genetics; D014162:Transfection / Q000379:methods*
D008055:Lipids; C086724:Lipofectamine
A549; Expression vector; Lipofectamine; Lipofection; Transfection