In vitro OECD test methods applied to screen the estrogenic effect of chemicals, used in Korea.
In this study, 27 chemicals found in household products, which became an issue in Korea were screened for the agonistoc and antagonistic effects against human estrogen receptor using official Organization for Economic Cooperation and Development (OECD) in vitro assays, STTA assay using ERα-HeLa-9903 cell line and BG1Luc ER TA assay. In the case of human ER agonist screening by two assays, all tested chemicals did not show agonist effect against ER. In ER antagonist test by BG1Luc ER TA assay, five surfactants α-dodecyl-ω-hydroxypoly(oxyethylene), alcohols C16-18 ethoxylated, nonylphenol, ethoxylated, 3,6,9,12,15,18,21-heptaoxatritriacontan-1-ol, and α-dodecyl-ω-hydroxypoly(oxy-1,2-ethanediyl)) were found to exhibit weak antagonistic activities. The agonist/antagonist effects against human estrogen receptor of various chemicals, used in Korea by OECD test guideline are reported in this study. These results indicated that two OECD in vitro assays will can be applied in Korea by screening of agonistic/antagonistic effects against human ER of various chemicals.
Food Safety Risk Assessment Division, National Institute of Food and Drug Safety Evaluation, Chungcheongbuk-do 363-700, Republic of Korea.
Hee-Seok
HS
Lee
Food Safety Risk Assessment Division, National Institute of Food and Drug Safety Evaluation, Chungcheongbuk-do 363-700, Republic of Korea.
Eun-Jung
EJ
Park
Food Safety Risk Assessment Division, National Institute of Food and Drug Safety Evaluation, Chungcheongbuk-do 363-700, Republic of Korea.
Songyi
S
Han
Food Safety Risk Assessment Division, National Institute of Food and Drug Safety Evaluation, Chungcheongbuk-do 363-700, Republic of Korea.
Gyeong-Yong
GY
Oh
Food Safety Risk Assessment Division, National Institute of Food and Drug Safety Evaluation, Chungcheongbuk-do 363-700, Republic of Korea.
Min-Hee
MH
Kim
Food Safety Risk Assessment Division, National Institute of Food and Drug Safety Evaluation, Chungcheongbuk-do 363-700, Republic of Korea.
Hui-Seung
HS
Kang
Food Safety Risk Assessment Division, National Institute of Food and Drug Safety Evaluation, Chungcheongbuk-do 363-700, Republic of Korea.
Jin-Hyang
JH
Suh
Food Safety Risk Assessment Division, National Institute of Food and Drug Safety Evaluation, Chungcheongbuk-do 363-700, Republic of Korea.
Jae-Ho
JH
Oh
Food Safety Risk Assessment Division, National Institute of Food and Drug Safety Evaluation, Chungcheongbuk-do 363-700, Republic of Korea.
Kwang-Soo
KS
Lee
Food Safety Risk Assessment Division, National Institute of Food and Drug Safety Evaluation, Chungcheongbuk-do 363-700, Republic of Korea.
Myung-Sil
MS
Hwang
Food Safety Risk Assessment Division, National Institute of Food and Drug Safety Evaluation, Chungcheongbuk-do 363-700, Republic of Korea.
Guiim
G
Moon
Food Safety Risk Assessment Division, National Institute of Food and Drug Safety Evaluation, Chungcheongbuk-do 363-700, Republic of Korea.
Jin-Hwan
JH
Hong
Food Safety Risk Assessment Division, National Institute of Food and Drug Safety Evaluation, Chungcheongbuk-do 363-700, Republic of Korea. Electronic address: hslee0515@gmail.com.
In-Gyun
IG
Hwang
D001681:Biological Assay / Q000379:methods*; D004965:Estrogen Antagonists / Q000494:pharmacology*; D004967:Estrogens / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D066298:In Vitro Techniques; D066150:Organisation for Economic Co-Operation and Development; D011960:Receptors, Estrogen / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D056910:Republic of Korea; D013501:Surface-Active Agents / Q000494:pharmacology*
D004965:Estrogen Antagonists; D004967:Estrogens; D011960:Receptors, Estrogen; D013501:Surface-Active Agents
Agonist; Antagonist; Household product; Human estrogen receptor; OECD test guideline