Visualization of perfusion changes with laser speckle contrast imaging using the method of motion history image.
Laser speckle contrast imaging (LSCI) is a real-time imaging modality reflecting microvascular perfusion. We report on the application of the motion history image (MHI) method on LSCI data obtained from the two hemispheres of a mouse. Through the generation of a single image, MHI stresses the microvascular perfusion changes. Our experimental results performed during a pinprick-triggered spreading depolarization demonstrate the effectiveness of MHI: MHI allows the visualization of perfusion changes without loss of resolution and definition. Moreover, MHI provides close results to the ones given by the generalized differences (GD) algorithm. However, MHI has the advantage of giving information on the temporal evolution of the perfusion variations, which GD does not.
Biomedical Optics Laboratory, Department of Applied Physics, Indian School of Mines, Dhanbad, 826004, Jharkhand, India. Electronic address: mohamedzaheer1@gmail.com.
Mohammad Zaheer
MZ
Ansari
University of Angers, LARIS, Laboratoire Angevin de Recherche en Ingénierie des Systèmes, 62 Avenue Notre-Dame du Lac, 49000 Angers, France.
Anne
A
Humeau-Heurtier
Department of Experimental Neurology, Charité University Medicine Berlin, Charitéplatz 1, 10117 Berlin, Germany.
Nikolas
N
Offenhauser
Department of Experimental Neurology, Charité University Medicine Berlin, Charitéplatz 1, 10117 Berlin, Germany; Department of Neurology, Charité University Medicine Berlin, Charitéplatz 1, 10117 Berlin, Germany; Center for Stroke Research, Charité University Medicine Berlin, Charitéplatz 1, 10117 Berlin, Germany.
Jens P
JP
Dreier
Biomedical Optics Laboratory, Department of Applied Physics, Indian School of Mines, Dhanbad, 826004, Jharkhand, India.
Anil Kumar
AK
Nirala
D000465:Algorithms; D000818:Animals; D001783:Blood Flow Velocity; D002560:Cerebrovascular Circulation; D054022:Cerebrum / Q000098:blood supply*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D017078:Laser-Doppler Flowmetry / Q000379:methods*; D051379:Mice; D008833:Microcirculation; D055420:Perfusion Imaging / Q000379:methods*; D011237:Predictive Value of Tests; D012039:Regional Blood Flow; D015203:Reproducibility of Results; D013997:Time Factors
Generalized differences; Laser speckle contrast imaging; Motion history image; Spreading depolarization