Rab27b is Involved in Lysosomal Exocytosis and Proteolipid Protein Trafficking in Oligodendrocytes.
Myelination by oligodendrocytes in the central nervous system requires coordinated exocytosis and endocytosis of the major myelin protein, proteolipid protein (PLP). Here, we demonstrated that a small GTPase, Rab27b, is involved in PLP trafficking in oligodendrocytes. We showed that PLP co-localized with Rab27b in late endosomes/lysosomes in oligodendrocytes. Short hairpin-mediated knockdown of Rab27b not only reduced lysosomal exocytosis but also greatly diminished the surface expression of PLP in oligodendrocytes. In addition, knockdown of Rab27b reduced the myelin-like membranes induced by co-culture of oligodendrocytes and neurons. Our data suggest that Rab27b is involved in myelin biogenesis by regulating PLP transport from late endosomes/lysosomes to the cell membrane in oligodendrocytes.
Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, China.
Yun-Tian
YT
Shen
Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, China.
Yun
Y
Gu
Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, China.
Wen-Feng
WF
Su
Xinglin College, Nantong University, Nantong, 226007, China.
Jing-Fei
JF
Zhong
Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, China.
Zi-Han
ZH
Jin
Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, China.
Xiao-Song
XS
Gu
Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, China. chengang6626@ntu.edu.cn.
Gang
G
Chen
D000818:Animals; D000831:Animals, Newborn; D061207:Calcium Ionophores / Q000494:pharmacology; D002402:Cathepsin D / Q000378:metabolism; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D018920:Coculture Techniques; D004622:Embryo, Mammalian; D005089:Exocytosis / Q000187:drug effects / Q000502:physiology*; D015759:Ionomycin / Q000494:pharmacology; D008247:Lysosomes / Q000187:drug effects / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D009836:Oligodendroglia / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D021381:Protein Transport / Q000187:drug effects; D011510:Proteolipids / Q000378:metabolism*; D034622:RNA Interference / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D020691:rab GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*
D061207:Calcium Ionophores; D011510:Proteolipids; D015759:Ionomycin; D002402:Cathepsin D; C512956:Rab27b protein, rat; D020691:rab GTP-Binding Proteins
Lysosome; Myelination; Neuron-glial interaction; Oligodendrocyte; Rab27b