Molecular cloning and expression analysis of Fem1b from oriental river prawn Macrobrachium nipponense.
Feminization-1 homolog b (Fem1b) is one of the genes essential for male development and play central roles in sex determination of Caenorhabditis elegans. In this study, we cloned and characterized the full-length Fem1b cDNA from the freshwater prawn Macrobrachium nipponense (MnFem1b) in different tissues and at different developmental stages. Real-time quantitative reverse polymerase chain reaction (RT-qPCR) showed that the MnFem1b gene was expressed in all investigated tissues, with the highest expression level found in the testes. The results revealed that the MnFem1b gene might play roles in aspects of development of the male prawn phenotype. The RT-qPCR also revealed that MnFem1b mRNA expression was significantly increased at 10 days after metamorphosis. The expression levels in all investigated tissues showed a certain degree of sexually dimorphism, the expression levels in males were significantly higher than those in females (P < 0.05). Notably, the highest expression of MnFem1b was found in the testes. The expression of MnFem1b in different tissues indicates that it plays multiple biological functions in M. nipponense.
Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, China.|Fisheries Research Center, Animal Research Corporation of the Ministry of Animal Resources, Khartoum, Sudan.
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Rahman
Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, China.|Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, China.
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Fu
Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, China.
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Qiao
Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, China.
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Jin
Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, China.
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Bai
Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, China.
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Zhang
Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, China.
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Jiang
Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, China.
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Liang
Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, China.
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Sun
Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, China.
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Gong
Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, China.
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Jiang
Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, China.
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Xiong
Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, China.
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Wu
D000595:Amino Acid Sequence / Q000235:genetics; D013734:Androgen-Insensitivity Syndrome / Q000235:genetics*; D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics*; D017173:Caenorhabditis elegans / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D018507:Gene Expression Regulation, Developmental; D008297:Male; D008675:Metamorphosis, Biological / Q000235:genetics*; D010053:Ovary / Q000254:growth & development / Q000378:metabolism; D033541:Palaemonidae / Q000235:genetics* / Q000254:growth & development; D010802:Phylogeny; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D019849:Sex Determination Processes
D060829:Arthropod Proteins; D018076:DNA, Complementary; D012333:RNA, Messenger