Light-sheet microscopy imaging of a whole cleared rat brain with Thy1-GFP transgene.
Whole-brain imaging with light-sheet fluorescence microscopy and optically cleared tissue is a new, rapidly developing research field. Whereas successful attempts to clear and image mouse brain have been reported, a similar result for rats has proven difficult to achieve. Herein, we report on creating novel transgenic rat harboring fluorescent reporter GFP under control of neuronal gene promoter. We then present data on clearing the rat brain, showing that FluoClearBABB was found superior over passive CLARITY and CUBIC methods. Finally, we demonstrate efficient imaging of the rat brain using light-sheet fluorescence microscopy.
Nencki Institute, Pasteura 3, 02-093 Warsaw, Poland.
Marzena
M
Stefaniuk
Institut de Ciencies Fotoniques (ICFO), Barcelona Institute of Science and Technology, 08860, Castelldefels (Barcelona), Spain.
Emilio J
EJ
Gualda
Nencki Institute, Pasteura 3, 02-093 Warsaw, Poland.
Monika
M
Pawlowska
Nencki Institute, Pasteura 3, 02-093 Warsaw, Poland.
Diana
D
Legutko
Nencki Institute, Pasteura 3, 02-093 Warsaw, Poland.
Paweł
P
Matryba
Nencki Institute, Pasteura 3, 02-093 Warsaw, Poland.
Paulina
P
Koza
Nencki Institute, Pasteura 3, 02-093 Warsaw, Poland.
Witold
W
Konopka
Nencki Institute, Pasteura 3, 02-093 Warsaw, Poland.
Dorota
D
Owczarek
Nencki Institute, Pasteura 3, 02-093 Warsaw, Poland.
Marcin
M
Wawrzyniak
Institut de Ciencies Fotoniques (ICFO), Barcelona Institute of Science and Technology, 08860, Castelldefels (Barcelona), Spain.
Pablo
P
Loza-Alvarez
Nencki Institute, Pasteura 3, 02-093 Warsaw, Poland.|Institut de Ciencies Fotoniques (ICFO), Barcelona Institute of Science and Technology, 08860, Castelldefels (Barcelona), Spain.
Leszek
L
Kaczmarek
D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging*; D001931:Brain Mapping / Q000379:methods*; D049452:Green Fluorescent Proteins / Q000235:genetics; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D009474:Neurons / Q000166:cytology; D011401:Promoter Regions, Genetic / Q000235:genetics; D051381:Rats; D055647:Rats, Transgenic; D017208:Rats, Wistar; D018800:Thy-1 Antigens / Q000235:genetics / Q000378:metabolism
D018800:Thy-1 Antigens; D049452:Green Fluorescent Proteins