A Shotgun Proteomic Approach Reveals That Fe Deficiency Causes Marked Changes in the Protein Profiles of Plasma Membrane and Detergent-Resistant Microdomain Preparations from Beta vulgaris Roots.
In the present study we have used label-free shotgun proteomic analysis to examine the effects of Fe deficiency on the protein profiles of highly pure sugar beet root plasma membrane (PM) preparations and detergent-resistant membranes (DRMs), the latter as an approach to study microdomains. Altogether, 545 proteins were detected, with 52 and 68 of them changing significantly with Fe deficiency in PM and DRM, respectively. Functional categorization of these proteins showed that signaling and general and vesicle-related transport accounted for approximately 50% of the differences in both PM and DRM, indicating that from a qualitative point of view changes induced by Fe deficiency are similar in both preparations. Results indicate that Fe deficiency has an impact in phosphorylation processes at the PM level and highlight the involvement of signaling proteins, especially those from the 14-3-3 family. Lipid profiling revealed Fe-deficiency-induced decreases in phosphatidic acid derivatives, which may impair vesicle formation, in agreement with the decreases measured in proteins related to intracellular trafficking and secretion. The modifications induced by Fe deficiency in the relative enrichment of proteins in DRMs revealed the existence of a group of cytoplasmic proteins that appears to be more attached to the PM in conditions of Fe deficiency.
Elain
E
Gutierrez-Carbonell
Daisuke
D
Takahashi
University of Hamburg , Biocenter Klein Flottbek, Ohnhorststrasse 18, 22609 Hamburg, Germany.
Sabine
S
Lüthje
Departamento de Biología Celular, Fisiología e Inmunología, Universidad de Córdoba , Campus de Rabanales, Edificio Severo Ochoa, Córdoba 14014, Spain.
José Antonio
JA
González-Reyes
Laboratoire de Biogenèse Membranaire, UMR 5200 CNRS-Université Bordeaux Segalen, Bâtiment A3, INRA Bordeaux Aquitaine , 71 Rue Edouard Borlaux, CS 20032, F-33140 Villenave d'Ornon, France.
Sébastien
S
Mongrand
Fundación ARAID , calle María de Luna 11, 50018 Zaragoza, Spain.
Bruno
B
Contreras-Moreira
Anunciación
A
Abadía
Matsuo
M
Uemura
Javier
J
Abadía
USDA-ARS Chindren's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine , 1100 Bates Street, Houston, Texas 77030, United States.
Ana Flor
AF
López-Millán
D027461:Beta vulgaris / Q000737:chemistry*; D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism; D000090463:Iron Deficiencies; D008055:Lipids / Q000032:analysis; D021962:Membrane Microdomains / Q000737:chemistry* / Q000378:metabolism; D010712:Phosphatidic Acids; D010766:Phosphorylation; D010940:Plant Proteins / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry; D040901:Proteomics / Q000379:methods*
D008055:Lipids; D010712:Phosphatidic Acids; D010940:Plant Proteins
Detergent-resistant microdomain; Iron deficiency; Lipidomics; Plasma membrane; Proteomics; Sugar beet