Evaluation of the Antibacterial Effects and Mechanism of Action of Protocatechualdehyde against Ralstonia solanacearum.
Protocatechualdehyde (PCA) is an important plant-derived natural product that has been associated with a wide variety of biological activities and has been widely used in medicine as an antioxidant, anti-aging and an anti-inflammatory agent. However, fewer reports concerning its antibacterial effects on plant-pathogenic bacteria exist. Therefore, in this study, protocatechualdehyde was evaluated for its antibacterial activity against plant pathogens along with the mechanism of its antibacterial action. PCA at 40 μg/mL was highly active against R. solanacearum and significantly inhibited its growth. The minimum bactericidal concentration and minimum inhibitory concentration values for PCA were 40 μg/mL and 20 μg/mL, respectively. Further investigation of the mechanism of action of PCA via transmission electron microscopy and biological assays indicated that the destruction of the cell structure, the shapes and the inhibition of biofilm formation were important. In addition, the application of PCA effectively reduced the incidence of bacterial wilt on tobacco under greenhouse conditions, and the control efficiency was as high as 92.01% at nine days after inoculation. Taken together, these findings suggest that PCA exhibits strong antibacterial activity against R. solanacearum and has the potential to be applied as an effective antibacterial agent for controlling bacterial wilt caused by R. solanacearum.
Laboratory of Natural Products Pesticides, College of Plant Protection, Southwest University, Chongqing 400715, China. lsl203lst@163.com.
Shili
S
Li
Laboratory of Natural Products Pesticides, College of Plant Protection, Southwest University, Chongqing 400715, China. trcmei@126.com.
Yanmei
Y
Yu
Laboratory of Natural Products Pesticides, College of Plant Protection, Southwest University, Chongqing 400715, China. chenhuanni521@126.com.
Juanni
J
Chen
Laboratory of Natural Products Pesticides, College of Plant Protection, Southwest University, Chongqing 400715, China. guobing425@163.com.
Bing
B
Guo
Laboratory of Natural Products Pesticides, College of Plant Protection, Southwest University, Chongqing 400715, China. ylwzling@163.com.
Liang
L
Yang
Laboratory of Natural Products Pesticides, College of Plant Protection, Southwest University, Chongqing 400715, China. dingw@swu.edu.cn.
Wei
W
Ding
D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D001547:Benzaldehydes / Q000737:chemistry* / Q000494:pharmacology; D002396:Catechols / Q000737:chemistry* / Q000494:pharmacology; D018551:Solanum lycopersicum / Q000187:drug effects / Q000382:microbiology; D008826:Microbial Sensitivity Tests; D010935:Plant Diseases / Q000382:microbiology*; D043368:Ralstonia solanacearum / Q000187:drug effects* / Q000472:pathogenicity; D014026:Nicotiana / Q000187:drug effects / Q000382:microbiology
D000900:Anti-Bacterial Agents; D001547:Benzaldehydes; D002396:Catechols; C005581:protocatechualdehyde
Ralstonia solanacearum; antibacterial activity; bacterial wilt; biofilm formation; protocatechualdehyde