Genomics of high molecular weight plasmids isolated from an on-farm biopurification system.
The use of biopurification systems (BPS) constitutes an efficient strategy to eliminate pesticides from polluted wastewaters from farm activities. BPS environments contain a high microbial density and diversity facilitating the exchange of information among bacteria, mediated by mobile genetic elements (MGEs), which play a key role in bacterial adaptation and evolution in such environments. Here we sequenced and characterized high-molecular-weight plasmids from a bacterial collection of an on-farm BPS. The high-throughput-sequencing of the plasmid pool yielded a total of several Mb sequence information. Assembly of the sequence data resulted in six complete replicons. Using in silico analyses we identified plasmid replication genes whose encoding proteins represent 13 different Pfam families, as well as proteins involved in plasmid conjugation, indicating a large diversity of plasmid replicons and suggesting the occurrence of horizontal gene transfer (HGT) events within the habitat analyzed. In addition, genes conferring resistance to 10 classes of antimicrobial compounds and those encoding enzymes potentially involved in pesticide and aromatic hydrocarbon degradation were found. Global analysis of the plasmid pool suggest that the analyzed BPS represents a key environment for further studies addressing the dissemination of MGEs carrying catabolic genes and pathway assembly regarding degradation capabilities.
Instituto de Biotecnología y Biología Molecular (IBBM), CONICET- Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calles 47 y 115 (1900) La Plata, Argentina.
María C
MC
Martini
Center for Biotechnology (CeBiTec), Bielefeld University, Institute for Genome Research and Systems Biology, D-33615 Bielefeld, Germany.
Daniel
D
Wibberg
Instituto de Biotecnología y Biología Molecular (IBBM), CONICET- Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calles 47 y 115 (1900) La Plata, Argentina.
Mauricio
M
Lozano
Instituto de Biotecnología y Biología Molecular (IBBM), CONICET- Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calles 47 y 115 (1900) La Plata, Argentina.
Gonzalo
G
Torres Tejerizo
Instituto de Biotecnología y Biología Molecular (IBBM), CONICET- Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calles 47 y 115 (1900) La Plata, Argentina.
Francisco J
FJ
Albicoro
Center for Biotechnology (CeBiTec), Bielefeld University, Institute for Genome Research and Systems Biology, D-33615 Bielefeld, Germany.
Sebastian
S
Jaenicke
Microbial Ecology, GELIFES, University of Groningen, The Netherland.
Jan Dirk
JD
van Elsas
Servicio Antimicrobianos, Departamento de Bacteriología, Instituto Nacional de Enfermedades Infecciosas-ANLIS Dr. Carlos G. Malbrán, Buenos Aires, Argentina.
Alejandro
A
Petroni
Instituto de Biomedicina y Biotecnología de Cantabria (IBBTEC), Universidad de Cantabria-Consejo Superior de Investigaciones Científicas (CSIC), 39011 Santander, Spain.
M Pilar
MP
Garcillán-Barcia
Instituto de Biomedicina y Biotecnología de Cantabria (IBBTEC), Universidad de Cantabria-Consejo Superior de Investigaciones Científicas (CSIC), 39011 Santander, Spain.
Fernando
F
de la Cruz
Center for Biotechnology (CeBiTec), Bielefeld University, Institute for Genome Research and Systems Biology, D-33615 Bielefeld, Germany.
Andreas
A
Schlüter
Center for Biotechnology (CeBiTec), Bielefeld University, Institute for Genome Research and Systems Biology, D-33615 Bielefeld, Germany.
Alfred
A
Pühler
Instituto de Biotecnología y Biología Molecular (IBBM), CONICET- Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calles 47 y 115 (1900) La Plata, Argentina.
Mariano
M
Pistorio
Instituto de Biotecnología y Biología Molecular (IBBM), CONICET- Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calles 47 y 115 (1900) La Plata, Argentina.
Antonio
A
Lagares
Instituto de Biotecnología y Biología Molecular (IBBM), CONICET- Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calles 47 y 115 (1900) La Plata, Argentina.
María F
MF
Del Papa
D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics*; D008970:Molecular Weight; D010957:Plasmids / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D012093:Replicon