Techniques for Investigating Molecular Toxicology of Nanomaterials.
Nanotechnology has been a rapidly developing field in the past few decades, resulting in the more and more exposure of nanomaterials to human. The increased applications of nanomaterials for industrial, commercial and life purposes, such as fillers, catalysts, semiconductors, paints, cosmetic additives and drug carriers, have caused both obvious and potential impacts on human health and environment. Nanotoxicology is used to study the safety of nanomaterials and has grown at the historic moment. Molecular toxicology is a new subdiscipline to study the interactions and impacts of materials at the molecular level. To better understand the relationship between the molecular toxicology and nanomaterials, this review summarizes the typical techniques and methods in molecular toxicology which are applied when investigating the toxicology of nanomaterials and include six categories: namely; genetic mutation detection, gene expression analysis, DNA damage detection, chromosomal aberration analysis, proteomics, and metabolomics. Each category involves several experimental techniques and methods.
Yanli
Y
Wang
Chenchen
C
Li
Chenjie
C
Yao
Lin
L
Ding
Zhendong
Z
Lei
Minghong
M
Wu
D005821:Genetic Techniques; D049329:Nanostructures / Q000633:toxicity*; D040901:Proteomics; D014116:Toxicology / Q000379:methods*