Serum prolidase activity and oxidative-antioxidative status in patients with developmental dysplasia of the hip and its relationship with radiographic severity.
BACKGROUND
We aimed to investigate serum prolidase activity and to investigate its association with oxidative-antioxidative status in patients with developmental dysplasia of the hip (DDH).


METHODS
Oxidative status parameters, including lipid hydroperoxide (LOOH), total oxidant status (TOS), and the oxidative stress index (OSI), and antioxidative status parameters, free sulfhydryl groups (Total -SH), and total antioxidative capacity (TAC), as well as serum prolidase activity were assessed in patients with DDH (n = 93), and in healthy controls (n = 82). The severity of dysplasia was evaluated according to the Tonnis grading system.


RESULTS
Serum prolidase activity and the oxidant parameters (LOOH, TOS, and OSI) were significantly higher and the antioxidant parameters (Total -SH and TAC) were significantly lower in patients with DDH compared to the controls (P < 0.005 for all). Serum prolidase activity was positively correlated with the Tonnis grade of DDH and LOOH, TOS, and OSI levels (P < 0.001 for all), but inversely correlated with total -SH and TAC levels (P < 0.001 for all).


CONCLUSIONS
Increased levels of serum prolidase activity, LOOH, TOS, and OSI, and decreased levels of total -SH and TAC, may be associated with DDH, and these parameters may be useful adjunctive tools to assess the severity of DDH.
a Department of Orthopaedics and Traumatology , Harran University School of Medicine , 63300 Sanliurfa , Turkey.
Mehmet Akif
MA
Altay
a Department of Orthopaedics and Traumatology , Harran University School of Medicine , 63300 Sanliurfa , Turkey.
Cemil
C
Ertürk
a Department of Orthopaedics and Traumatology , Harran University School of Medicine , 63300 Sanliurfa , Turkey.
Ali
A
Levent
a Department of Orthopaedics and Traumatology , Harran University School of Medicine , 63300 Sanliurfa , Turkey.
Baki Volkan
BV
Çetin
b Department of Clinical Biochemistry , Harran University School of Medicine , 63300 Sanliurfa , Turkey.
Nurten
N
Aksoy
D000975:Antioxidants / Q000378:metabolism*; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D004150:Dipeptidases / Q000097:blood*; D005260:Female; D006618:Hip Dislocation, Congenital / Q000097:blood* / Q000000981:diagnostic imaging* / Q000378:metabolism; D006801:Humans; D007223:Infant; D008054:Lipid Peroxides / Q000097:blood; D008297:Male; D016877:Oxidants / Q000097:blood; D018384:Oxidative Stress
D000975:Antioxidants; D008054:Lipid Peroxides; D016877:Oxidants; D004150:Dipeptidases; C022449:proline dipeptidase
Developmental dysplasia of the hip; Prolidase enzyme activity; Total antioxidant capacity; Total free sulfhydryl groups; Total oxidant status