Cavin4b/Murcb Is Required for Skeletal Muscle Development and Function in Zebrafish.
Skeletal muscles provide metazoans with the ability to feed, reproduce and avoid predators. In humans, a heterogeneous group of genetic diseases, termed muscular dystrophies (MD), lead to skeletal muscle dysfunction. Mutations in the gene encoding Caveolin-3, a principal component of the membrane micro-domains known as caveolae, cause defects in muscle maintenance and function; however it remains unclear how caveolae dysfunction underlies MD pathology. The Cavin family of caveolar proteins can form membrane remodeling oligomers and thus may also impact skeletal muscle function. Changes in the distribution and function of Cavin4/Murc, which is predominantly expressed in striated muscles, have been reported to alter caveolae structure through interaction with Caveolin-3. Here, we report the generation and phenotypic analysis of murcb mutant zebrafish, which display impaired swimming capacity, skeletal muscle fibrosis and T-tubule abnormalities during development. To understand the mechanistic importance of Murc loss of function, we assessed Caveolin-1 and 3 localization and found it to be abnormal. We further identified an in vivo function for Murc in Erk signaling. These data link Murc with developmental defects in T-tubule formation and progressive muscle dysfunction, thereby providing a new candidate for the etiology of muscular dystrophy.
Department of Biochemistry and Biophysics, University of California, San Francisco (UCSF), San Francisco, California, United States of America.|Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany.
Michael P
MP
Housley
Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany.
Brian
B
Njaine
Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany.
Filomena
F
Ricciardi
Department of Biochemistry and Biophysics, University of California, San Francisco (UCSF), San Francisco, California, United States of America.|Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany.
Oliver A
OA
Stone
Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany.
Soraya
S
Hölper
Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany.
Marcus
M
Krüger
Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany.
Sawa
S
Kostin
Department of Biochemistry and Biophysics, University of California, San Francisco (UCSF), San Francisco, California, United States of America.|Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany.
Didier Y R
DY
Stainier
D000818:Animals; D030801:Animals, Genetically Modified; D021941:Caveolae / Q000378:metabolism; D051242:Caveolin 1 / Q000378:metabolism; D051244:Caveolin 3 / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D007473:Ion Channels / Q000235:genetics; D024510:Muscle Development / Q000235:genetics*; D009124:Muscle Proteins / Q000235:genetics* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000196:embryology* / Q000473:pathology*; D009136:Muscular Dystrophies / Q000235:genetics; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics / Q000378:metabolism; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism
D051242:Caveolin 1; D051244:Caveolin 3; D007473:Ion Channels; D009124:Muscle Proteins; D029961:Zebrafish Proteins; C000616314:cavin4b protein, zebrafish; D048049:Extracellular Signal-Regulated MAP Kinases