Modelling of different enzyme productions by solid-state fermentation on several agro-industrial residues.
A simple kinetic model, with only three fitting parameters, for several enzyme productions in Petri dishes by solid-state fermentation is proposed in this paper, which may be a valuable tool for simulation of this type of processes. Basically, the model is able to predict temporal fungal enzyme production by solid-state fermentation on complex substrates, maximum enzyme activity expected and time at which these maxima are reached. In this work, several fermentations in solid state were performed in Petri dishes, using four filamentous fungi grown on different agro-industrial residues, measuring xylanase, exo-polygalacturonase, cellulose and laccase activities over time. Regression coefficients after fitting experimental data to the proposed model turned out to be quite high in all cases. In fact, these results are very interesting considering, on the one hand, the simplicity of the model and, on the other hand, that enzyme activities correspond to different enzymes, produced by different fungi on different substrates.
Laboratory of Microbiology, Faculty of Marine and Environmental Sciences, University of Cádiz, Pol. Río San Pedro s/n, Puerto Real, Spain. anabelen.diaz@uca.es.|Department of Chemical Engineering and Food Technology, Faculty of Sciences, International Agro-Food Campus of Excellence (CeiA3), University of Cádiz, Pol. Río San Pedro s/n, Puerto Real, Spain. anabelen.diaz@uca.es.
Ana Belen
AB
Diaz
Department of Chemical Engineering and Food Technology, Faculty of Sciences, International Agro-Food Campus of Excellence (CeiA3), University of Cádiz, Pol. Río San Pedro s/n, Puerto Real, Spain.
Ana
A
Blandino
Department of Chemical Engineering, School of Chemical Engineering & Analytical Science, University of Manchester, C77, The Mill, Oxford Road, Manchester, M13 9PL, UK.
Colin
C
Webb
Department of Chemical Engineering and Food Technology, Faculty of Sciences, International Agro-Food Campus of Excellence (CeiA3), University of Cádiz, Pol. Río San Pedro s/n, Puerto Real, Spain.
Ildefonso
I
Caro
D003470:Culture Media / Q000737:chemistry*; D004798:Enzymes / Q000032:analysis*; D005285:Fermentation; D005658:Fungi / Q000201:enzymology* / Q000378:metabolism*; D008962:Models, Theoretical; D013997:Time Factors
D003470:Culture Media; D004798:Enzymes
Agro-industrial residues; Enzymes; Kinetic; Solid-state fermentation