In Vitro Antibacterial Activity of Curcumin-Polymyxin B Combinations against Multidrug-Resistant Bacteria Associated with Traumatic Wound Infections.
Bacterial infections resulting from nonsurgical traumatic wounds can be life threatening, especially those caused by multidrug-resistant (MDR) bacteria with limited therapeutic options. The antimicrobial activity of polymyxin B (1) and curcumin (2) alone and in combination was determined versus MDR bacterial isolates associated with traumatic wound infections. Cytotoxicity assays for 1 and 2 were undertaken in keratinocyte cell lines. Minimum inhibitory concentrations of 1 were significantly reduced in the presence of 2 (3- to 10-fold reduction), with synergy observed. Time-kill assays showed the combinations produced bactericidal activity. Cytotoxicity assays indicate the toxicity of 2 was reduced in the presence of 1.
School of Veterinary Medicine, Faculty of Health and Medical Sciences, University of Surrey , Guildford GU2 7AL, U.K.
Jonathan W
JW
Betts
Amir S
AS
Sharili
School of Veterinary Medicine, Faculty of Health and Medical Sciences, University of Surrey , Guildford GU2 7AL, U.K.
Roberto M
RM
La Ragione
David W
DW
Wareham
D040981:Acinetobacter baumannii / Q000187:drug effects; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003474:Curcumin / Q000494:pharmacology*; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects*; D004357:Drug Synergism; D006801:Humans; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D011112:Polymyxin B / Q000494:pharmacology*; D012867:Skin / Q000473:pathology; D014946:Wound Infection / Q000188:drug therapy*
D000900:Anti-Bacterial Agents; D003474:Curcumin; D011112:Polymyxin B