Alleviation of spinal cord injury by Ginkgolide B via the inhibition of STAT1 expression.
Ginkgolide B has been known to inhibit cell apoptosis by modulating multiple cytokines and plays an important role in neuroprotection. Signal transducer and activator of transcription 1 (STAT1) has been studied in a spinal cord injury (SCI) model. However, the role of Ginkgolide B in SCI treatment remains unclear. This study investigated the potential mechanism of Ginkgolide B using an SCI rat model. SD rats were used to generate an SCI model followed by Ginkgolide B injection (4 mg/kg) for 14 days. Spinal cord tissue samples were examined using hematoxylin and eosin (H&E) staining. The expression of STAT1 was determined by western blot. Using a dyskinesia scale, intervention with Ginkgolide B significantly decreased the severity of SCI. H&E staining revealed less nuclear condensation and cell necrosis in SCI rats after treatment with Ginkgolide B. STAT1 expression was significantly increased in SCI model rats, but was lower after Ginkgolide B treatment. Therefore, Ginkgolide B can effectively inhibit STAT1 expression and alleviate SCI.
Department of Orthopedics Surgery, Jinan Military General Hospital, Jinan, Shandong, China.
J L
JL
Zheng
Department of Orthopedics Surgery, Jinan Military General Hospital, Jinan, Shandong, China.
B S
BS
Li
Department of Orthopedics Surgery, Jinan Military General Hospital, Jinan, Shandong, China.
X C
XC
Cao
Department of Orthopedics Surgery, Jinan Military General Hospital, Jinan, Shandong, China.
W K
WK
Zhuo
Department of Orthopedics Surgery, Jinan Military General Hospital, Jinan, Shandong, China.
G
G
Zhang
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D004195:Disease Models, Animal; D046934:Ginkgolides / Q000494:pharmacology*; D007783:Lactones / Q000494:pharmacology*; D018696:Neuroprotective Agents / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000187:drug effects; D050794:STAT1 Transcription Factor / Q000037:antagonists & inhibitors* / Q000096:biosynthesis*; D013119:Spinal Cord Injuries / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology
D046934:Ginkgolides; D007783:Lactones; D018696:Neuroprotective Agents; D050794:STAT1 Transcription Factor; C494081:Stat1 protein, rat; C045856:ginkgolide B