The role of nuclear PKMζ in memory maintenance.
Recently, protein kinase M ζ (PKMζ) has emerged as an important player for maintaining memory. It has been reported that PKMζ regulates the trafficking of GluA2 in postsynaptic membranes to maintain memory. However, there has been no study on PKMζ outside the synaptic region regarding memory maintenance. Here, we found that PKMζ is transported to the nucleus in a neural activity-dependent manner. Moreover, we found that PKMζ phosphorylates CREB-binding protein (CBP) at serine residues and that PKMζ inhibition reduces the acetylation of histone H2B and H3. Finally, we showed that the amnesic effect of PKMζ inhibition can be rescued by enhancing histone acetylation level. These results suggest the possibility that nuclear PKMζ has a crucial role in memory maintenance.
School of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul 08826, South Korea.
Hyoung-Gon
HG
Ko
School of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul 08826, South Korea.
Ji-Il
JI
Kim
School of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul 08826, South Korea.
Su-Eon
SE
Sim
School of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul 08826, South Korea.
TaeHyun
T
Kim
School of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul 08826, South Korea.
Juyoun
J
Yoo
School of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul 08826, South Korea.
Sun-Lim
SL
Choi
School of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul 08826, South Korea.
Sung Hee
SH
Baek
Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul, South Korea.
Won-Jin
WJ
Yu
Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul, South Korea.
Jong-Bok
JB
Yoon
Departments of Physiology and Pharmacology, Anesthesiology, and Neurology, The Robert F. Furchgott Center for Neural and Behavioral Science, State University of New York Downstate Medical Center, Brooklyn, NY 11203, USA.
Todd Charlton
TC
Sacktor
School of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul 08826, South Korea. Electronic address: kaang@snu.ac.kr.
Bong-Kiun
BK
Kaang
D000647:Amnesia / Q000378:metabolism* / Q000503:physiopathology; D000679:Amygdala / Q000378:metabolism* / Q000503:physiopathology; D000818:Animals; D001522:Behavior, Animal / Q000502:physiology; D050882:CREB-Binding Protein / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism*; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005786:Gene Expression Regulation / Q000502:physiology*; D057809:HEK293 Cells; D006624:Hippocampus / Q000378:metabolism; D006801:Humans; D008297:Male; D008568:Memory / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000099221:Protein Kinase C zeta
D050882:CREB-Binding Protein; D011493:Protein Kinase C; D000099221:Protein Kinase C zeta; C494364:Crebbp protein, mouse
CREB-binding protein; Histone acetylation; Memory maintenance; PKMζ; Translocation into nucleus