Biotinylated vanadium and chromium sulfide nanoparticles as probes for colocalization of membrane proteins.
We report the microemulsion synthesis of vanadium and chromium sulfide nanoparticles (NPs) and their biological application as nanoprobes for colocalization of membrane proteins. Spherical V2 S3 and Cr2 S3 NPs were prepared in reverse microemulsion droplets, as nanoreactors, obtained by the surfactant sodium bis(2-ethylhexyl) sulfosuccinate (AOT) in nonpolar organic phase (heptane). Electron microscopic data indicated that the size distribution of the nanoparticles was uniform with an average diameter between 3 ÷ 5 nm. The prepared hydrophobic nanocrystals were transferred in aqueous phase by surface cap exchange of AOT with biotin-dihydrolipoic ligands. This substitution allows the nanoparticles solubility in aqueous solutions and confer their bioactivity. In addition, we report the conjugation procedure between α-Lipoic acid (LA) and biotin (abbreviated as biotin-LA). The biotin-LA structure was characterized by 1D and 2D NMR spectroscopy. The biotinylated vanadium and chromium sulfide nanoparticles were tested as probes for colocalization of glutamate receptors on sodium-dodecyl-sulfate-digested replica prepared from rat hippocampus. The method suggests their high labeling efficiency for study of membrane biological macromolecules. Microsc. Res. Tech. 79:799-805, 2016. © 2016 Wiley Periodicals, Inc.
Graduate School of Science and Engineering, Saitama University, Shimo-Ohkubo 255, Sakura Ku, Saitama, 338-8570, Japan.|Department of Engineering Geoecology, Laboratory of Engineering NanoBiotechnology, University of Mining and Geology "St. Ivan Rilski", Bulgaria.
Alexandre
A
Loukanov
Materials Research Laboratory, University of Nova Gorica, SI-5000, Slovenia.
Saim
S
Emin
D001710:Biotin / Q000737:chemistry*; D019921:Biotinylation; D002857:Chromium / Q000737:chemistry*; D008565:Membrane Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D008954:Models, Biological; D015335:Molecular Probes / Q000138:chemical synthesis* / Q000737:chemistry* / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry*; D013386:Succinates; D014639:Vanadium / Q000737:chemistry*
C512554:1,4-bis(2-ethylhexyl) sodium sulfosuccinate; D008565:Membrane Proteins; D015335:Molecular Probes; D013386:Succinates; D014639:Vanadium; D002857:Chromium; D001710:Biotin
biotin-dihydrolipoic conjugate; membrane proteins; vanadium and chromium sulfide nanoparticles