The Expression of VHL (Von Hippel-Lindau) After Traumatic Spinal Cord Injury and Its Role in Neuronal Apoptosis.
The VHL (Von Hippel-Lindau) gene is a tumor suppressor gene, which is best known as an E3 ubiquitin ligase that negatively regulates the hypoxia inducible factor. The inactivation of VHL gene could result in the abnormal synthesis of VHL protein, which is in contact with the development and occurrence of renal clear cell carcinoma. However, the expression and possible function of VHL in central nervous system (CNS) is still unclear. To examine the function of VHL in CNS injury and repair, we used an acute spinal cord injury (SCI) model in adult rats. Western blot analysis showed an important upregulation of VHL protein, reaching a peak at day 3 and then declined during the following days. Double immunofluorescence staining showed that VHL was co-expressed with neurons, but not with astrocytes and microglia. Moreover, we detected that active caspase-3 had co-localized with VHL in neurons after SCI. Additionally in vitro, VHL depletion, by short interfering RNA, significantly reduced neuronal apoptosis. In conclusion, these data suggested that the change of VHL protein expression was related to neuronal apoptosis after SCI.
Medical College, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.|Department of Orthopedics, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, 226001, People's Republic of China.
Jie
J
Hao
Department of Orthopedics, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, 226001, People's Republic of China.
Xiaoqing
X
Chen
School of Nursing, Nantong University, Nantong, People's Republic of China.
Ting
T
Fu
Medical College, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.|Department of Orthopedics, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, 226001, People's Republic of China.
Jie
J
Liu
Medical College, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.|Department of Orthopedics, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, 226001, People's Republic of China.
Mingchen
M
Yu
Medical College, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.|Department of Orthopedics, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, 226001, People's Republic of China.
Wei
W
Han
The Second Affiliated Hospital, Nantong University, Nantong, 226001, People's Republic of China.
Shuang
S
He
Medical College, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.|Department of Orthopedics, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, 226001, People's Republic of China.
Rong
R
Qian
Department of Orthopedics, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, 226001, People's Republic of China. renjianbing2016@163.com.
Feng
F
Zhang
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D053148:Caspase 3 / Q000378:metabolism; D008025:Ligases / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D015854:Up-Regulation; D051794:Von Hippel-Lindau Tumor Suppressor Protein / Q000378:metabolism*
D051794:Von Hippel-Lindau Tumor Suppressor Protein; C505410:Casp3 protein, rat; D053148:Caspase 3; D008025:Ligases
Apoptosis; Neuron; Spinal cord injury; Von Hippel-Lindau