Long non-coding RNA HULC as a novel serum biomarker for diagnosis and prognosis prediction of gastric cancer.
Long non-coding RNAs (lncRNAs) have recently emerged as vital players in tumor biology with potential value in cancer diagnosis, prognosis, and therapeutics. The lncRNA HULC (highly up-regulated in liver cancer) is increased in many malignancies, yet its serum expression profile and clinical value in gastric cancer (GC) patients remain unclear. Quantitative real-time polymerase chain reaction (RT-qPCR) for large-scale analysis of the serum expression of HULC in GC patients reliably detected circulating HULC and revealed that it is upregulated in GC patients. A high serum HULC level correlated with tumor size, lymph node metastasis, distant metastasis, tumor-node-metastasis stage, and H. pylori infection. The area under the ROC curve for HULC was up to 0.888, which was higher than that for CEA (0.694) and CA72-4 (0.514). Follow-up detection and Kaplan-Meier curve analysis revealed HULC is a good predictor of GC prognosis. Our present study indicates that circulating HULC may represent a novel serum tumor marker for early diagnosis and monitoring progression and prognosis of GC.
Medical School of Medicine, Nantong University, Nantong 226000, Jiangsu Province, China.
Chunjing
C
Jin
Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, Nantong 226000, Jiangsu Province, China.
Wei
W
Shi
Laboratory Medicine Center, Affiliated Hospital of Nantong University, Nantong 226000, Jiangsu Province, China.
Feng
F
Wang
Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, Nantong 226000, Jiangsu Province, China.
Xianjuan
X
Shen
Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, Nantong 226000, Jiangsu Province, China.
Jing
J
Qi
Laboratory Medicine Center, Affiliated Hospital of Nantong University, Nantong 226000, Jiangsu Province, China.
Hui
H
Cong
Medical School of Medicine, Nantong University, Nantong 226000, Jiangsu Province, China.
Jie
J
Yuan
Medical School of Medicine, Nantong University, Nantong 226000, Jiangsu Province, China.
Linying
L
Shi
Medical School of Medicine, Nantong University, Nantong 226000, Jiangsu Province, China.
Bingying
B
Zhu
Medical School of Medicine, Nantong University, Nantong 226000, Jiangsu Province, China.
Xi
X
Luo
Medical School of Medicine, Nantong University, Nantong 226000, Jiangsu Province, China.
Yan
Y
Zhang
Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, Nantong 226000, Jiangsu Province, China.|Laboratory Medicine Center, Affiliated Hospital of Nantong University, Nantong 226000, Jiangsu Province, China.
Shaoqing
S
Ju
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics; D016022:Case-Control Studies; D002478:Cells, Cultured; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D062085:RNA, Long Noncoding / Q000097:blood* / Q000235:genetics; D013274:Stomach Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology
D014408:Biomarkers, Tumor; C574685:HULC long non-coding RNA, human; D062085:RNA, Long Noncoding
HULC; biomarker; gastric cancer; long non-coding RNA