Predictive recognition of native proteins by cucurbit[7]uril in a complex mixture.
The recognition of human growth hormone (hGH) by the synthetic host molecule cucurbit[7]uril (Q7) was predicted on the basis of its N-terminal phenylalanine. An aqueous-compatible resin with covalently immobilized Q7 groups was prepared and shown to recognize native insulin and hGH in simple and complex mixtures.
Department of Chemistry, Trinity University, San Antonio, TX 78212, USA. aurbach@trinity.edu.
Wei
W
Li
Department of Chemistry, Trinity University, San Antonio, TX 78212, USA. aurbach@trinity.edu.
Andrew T
AT
Bockus
Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA.
Brittany
B
Vinciguerra
Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA.
Lyle
L
Isaacs
Department of Chemistry, Trinity University, San Antonio, TX 78212, USA. aurbach@trinity.edu.
Adam R
AR
Urbach
D001952:Bridged-Ring Compounds / Q000378:metabolism*; D019382:Human Growth Hormone / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007093:Imidazoles / Q000378:metabolism*; D008958:Models, Molecular; D011485:Protein Binding; D011487:Protein Conformation; D047028:Macrocyclic Compounds; D000099246:Cucurbit(n)urils
D001952:Bridged-Ring Compounds; D019382:Human Growth Hormone; D007093:Imidazoles; C456276:cucurbit(7)uril; D047028:Macrocyclic Compounds; D000099246:Cucurbit(n)urils