Inflated Sporopollenin Exine Capsules Obtained from Thin-Walled Pollen.
Sporopollenin is a physically robust and chemically resilient biopolymer that comprises the outermost layer of pollen walls and is the first line of defense against harsh environmental conditions. The unique physicochemical properties of sporopollenin increasingly motivate the extraction of sporopollenin exine capsules (SECs) from pollen walls as a renewable source of organic microcapsules for encapsulation applications. Despite the wide range of different pollen species with varying sizes and wall thicknesses, faithful extraction of pollen-mimetic SECs has been limited to thick-walled pollen capsules with rigid mechanical properties. There is an unmet need to develop methods for producing SECs from thin-walled pollen capsules which constitute a large fraction of all pollen species and have attractive materials properties such as greater aerosol dispersion. Herein, we report the first successful extraction of inflated SEC microcapsules from a thin-walled pollen species (Zea mays), thereby overcoming traditional challenges with mechanical stability and loss of microstructure. Morphological and compositional characterization of the SECs obtained by the newly developed extraction protocol confirms successful protein removal along with preservation of nanoscale architectural features. Looking forward, there is excellent potential to apply similar strategies across a wide range of unexplored thin-walled pollen species.
School of Materials Science and Engineering Nanyang Technological University, 50 Nanyang Avenue 639798, Singapore.|Centre for Biomimetic Sensor Science, Nanyang Technological University, 50 Nanyang Drive 637553, Singapore.
Jae Hyeon
JH
Park
School of Materials Science and Engineering Nanyang Technological University, 50 Nanyang Avenue 639798, Singapore.|Centre for Biomimetic Sensor Science, Nanyang Technological University, 50 Nanyang Drive 637553, Singapore.
Jeongeun
J
Seo
School of Materials Science and Engineering Nanyang Technological University, 50 Nanyang Avenue 639798, Singapore.|Centre for Biomimetic Sensor Science, Nanyang Technological University, 50 Nanyang Drive 637553, Singapore.
Joshua A
JA
Jackman
School of Materials Science and Engineering Nanyang Technological University, 50 Nanyang Avenue 639798, Singapore.|Centre for Biomimetic Sensor Science, Nanyang Technological University, 50 Nanyang Drive 637553, Singapore.|School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive 637459, Singapore.
Nam-Joon
NJ
Cho
D001704:Biopolymers / Q000737:chemistry*; D002214:Capsules; D002338:Carotenoids / Q000737:chemistry*; D002473:Cell Wall / Q000737:chemistry; D006868:Hydrolysis; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D011058:Pollen / Q000737:chemistry*; D003313:Zea mays / Q000737:chemistry*
D001704:Biopolymers; D002214:Capsules; C009800:sporopollenin; D002338:Carotenoids