[Arctiin ameliorates advanced oxidation protein product-induced epithelial-to- mesenchymal transition in HK-2 cells by inhibiting endoplasmic reticulum stress].
OBJECTIVE
To investigate the effect of arctiin on advanced oxidation protein product (AOPP)-induced epithelial-to-mesenchymal transition (EMT) in tubular cells and explore the mechanisms underlying this effect.


METHODS
Human proximal tubular cells (HK-2 cells) were treated with bovine serum albumin (BSA) or AOPPs in the presence or absence of arctiin. The expressions of E-cadherin, vimentin, and GRP78 at the protein and mRNA levels in the cells were examined using Western blotting and quantitative real-time PCR. The level of reactive oxygen species (ROS) was measured by flow cytometry with DCFH-DA as the fluorescent probe.


RESULTS
Compared with BSA-treated cells, the cells treated with AOPPs showed decreased expression of epithelial cell marker E-cadherin and overexpression of mesenchymal marker vimentin and endoplasmic reticulum stress marker GRP78 with an increased ROS level. These changes induced by AOPPs were partly inhibited by arctiin.


CONCLUSIONS
Arctiin can ameliorate AOPP-induced EMT in tubular cells by inhibiting endoplasmic reticulum stress, and oxidative stress response may participate in this process.
Department of Nephrology, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China. E-mail: gzh_zj@qq.com.
Jun
J
Zhang
Li-Li
LL
Huang
Xiu-Jie
XJ
Liang
Yue
Y
Wang
Na
N
Duan
Xiao-Hong
XH
Xiang
Shuang-Shuang
SS
Shu
Ting-Ting
TT
Guo
Lei
L
Yang
Xun
X
Tang
D062107:Advanced Oxidation Protein Products / Q000009:adverse effects*; D015703:Antigens, CD; D015820:Cadherins / Q000378:metabolism; D002460:Cell Line; D000091342:Endoplasmic Reticulum Chaperone BiP; D059865:Endoplasmic Reticulum Stress; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects; D058750:Epithelial-Mesenchymal Transition; D005663:Furans / Q000494:pharmacology*; D005960:Glucosides / Q000494:pharmacology*; D006360:Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D007684:Kidney Tubules / Q000166:cytology* / Q000187:drug effects; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism; D014746:Vimentin / Q000378:metabolism
D062107:Advanced Oxidation Protein Products; D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; D000091342:Endoplasmic Reticulum Chaperone BiP; D005663:Furans; D005960:Glucosides; C000717247:HSPA5 protein, human; D006360:Heat-Shock Proteins; D017382:Reactive Oxygen Species; D014746:Vimentin; C077992:arctiin