In situ cultured preantral follicles is a useful model to evaluate the effect of anticancer drugs on caprine folliculogenesis.
Despite the increase in the incidence of cancer, the number of women who survive cancer treatment is growing. However, one of the principal results of chemotherapy is premature ovarian failure (POF). The aim of this study was to use the in situ culture preantral follicles as an in vitro model to evaluate the toxicity of two anticancer drugs, doxorubicin (DXR) and paclitaxel (PTX), on the integrity and development of ovarian follicles. Fragments of the ovarian cortex of goats were cultured in vitro for 1 or 7 days in α-MEM(+) supplemented with different concentrations of DXR (0.003, 0.03, or 0.3 µg/mL) and PTX (0.001, 0.01, or 0.1 µg/mL). Analyses were performed before and after culture to evaluate tissue integrity by classical histology, apoptosis by TUNEL assay, DNA laddering kit and the detection of activated caspase 3, and DNA damage by the immune detection of phosphorylated histone H2A.x (H2AXph139). Both DXR and PTX reduced the number of morphologically normal primordial and developing follicles. Positive staining for TUNEL and active caspase 3 was detected in all the samples (P < 0.05). Therefore, we propose the in situ culture of caprine preantral follicles as a useful experimental model for assessing the toxic effects of the chemotherapeutic agents on ovarian folliculogenesis. Microsc. Res. Tech. 79:773-781, 2016. © 2016 Wiley Periodicals, Inc.
Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceará, Avenue Parajana 1700, Fortaleza, CE, 60740-000, Brazil.
Denise Damasceno
DD
Guerreiro
Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceará, Avenue Parajana 1700, Fortaleza, CE, 60740-000, Brazil.
Laritza Ferreira de
http://orcid.org/0000-0001-6391-7113
LF
Lima
Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceará, Avenue Parajana 1700, Fortaleza, CE, 60740-000, Brazil.
Giovanna Quintino
GQ
Rodrigues
Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceará, Avenue Parajana 1700, Fortaleza, CE, 60740-000, Brazil.
Adeline de Andrade
Ade A
Carvalho
Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceará, Avenue Parajana 1700, Fortaleza, CE, 60740-000, Brazil.
Simone Vieira
SV
Castro
Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceará, Avenue Parajana 1700, Fortaleza, CE, 60740-000, Brazil.
Cláudio Cabral
CC
Campello
Department Pharmacology, Federal University of Ceará, Fortaleza, CE, 60430-270, Brazil.
Cláudia do Ó
Cdo Ó
Pessoa
Department of Animal Science, Federal University of Ceará, Fortaleza, CE, 60430-270, Brazil.
Carla Renata Figueiredo
CR
Gadelha
Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceará, Avenue Parajana 1700, Fortaleza, CE, 60740-000, Brazil.
José Ricardo de
JR
Figueiredo
Department of Animal Science, McGill University, Ste-Anne-de-Bellevue, Quebec, Canada.
Vilceu
V
Bordignon
Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceará, Avenue Parajana 1700, Fortaleza, CE, 60740-000, Brazil.
Ana Paula Ribeiro
AP
Rodrigues
D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000737:chemistry; D053938:DNA Fragmentation / Q000187:drug effects; D004317:Doxorubicin / Q000633:toxicity; D005260:Female; D006041:Goats; D006657:Histones / Q000737:chemistry; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008954:Models, Biological; D009866:Oogenesis / Q000187:drug effects*; D006080:Ovarian Follicle / Q000187:drug effects*; D017239:Paclitaxel / Q000633:toxicity; D018675:Toxicity Tests
D000970:Antineoplastic Agents; D006657:Histones; D004317:Doxorubicin; D053148:Caspase 3; D017239:Paclitaxel
apoptosis; chemotherapy; doxorubicin; paclitaxel; preantral follicle