Development of a new device for artificial insemination in cynomolgus macaques.
In cynomolgus macaques, an important animal species for biomedical research, efficient reproduction has been hampered partly due to the difficulties of artificial insemination (AI) using straw tubes developed for humans or farm animals, because cynomolgus macaques have a complex cervical canal structure. In this study, taking into consideration the unique structure of the macaque cervical canal, we developed a novel device for AI, comprised of a syringe and an outer cylinder. At 24 and 48 h after using this device to inject semen into one female, viable sperm were observed in the oviduct where the sperm meets the oocytes. We then attempted AI using this new device on 10 females that were at pre-ovulation, and pregnancy was successful in three animals (30% pregnancy rate). These results show that the newly developed device can be used for AI in cynomolgus macaques.
Drug Safety Research Center (DSR), Shin Nippon Biomedical Laboratories (SNBL), Ltd., Kagoshima 891-1394, Japan.
Shuzo
S
Koyama
Koji
K
Fukuda
Sho
S
Watanabe
Sakae
S
Kohara
Hideaki
H
Tsuchiya
Koichiro
K
Fukuzaki
Masashi
M
Nagano
Yasuhiro
Y
Uno
Yoshihiko
Y
Hosoi
D000818:Animals; D015925:Cryopreservation / Q000379:methods; D004867:Equipment Design; D005260:Female; D007315:Insemination, Artificial / Q000295:instrumentation* / Q000379:methods* / Q000662:veterinary; D008252:Macaca fascicularis; D008297:Male; D009865:Oocytes / Q000166:cytology*; D010060:Ovulation; D011247:Pregnancy; D018873:Pregnancy Rate; D012661:Semen; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology*