Association Between the G20210A Polymorphism of Prothrombin Gene and Myocardial Infarction in Tunisian Population.
The prothrombin is the precursor of the serine protease thrombin, a key enzyme in homeostasis. Prothrombin G20210A polymorphism (rs1799963) was described as a moderate risk factor for venous thrombosis because this mutation is associated with prothrombin elevated levels which may lead to an imbalance between the procoagulant, anticoagulant, and fibrinolytic system. 20210A carriers have an increased risk of thrombosis. In this study, we proposed to determine the prevalence of 20210A prothrombin variant among Tunisian population, and to evaluate the potential relevance of this variant with myocardial infarction. This study included 1290 unrelated Tunisians (1007 male and 283 female) divided in two groups: Four hundred and eighty-seven MI patients (mean age: 52.64 ± 8.98 years) and 803 apparently healthy controls (mean age: 51 ± 8.99). The prothrombin G20210A polymorphism was carried out by polymerase chain reaction/restriction fragment length polymorphism (PCR-RFLP) analysis. The distribution of genotypes was in accordance with Hardy-Weinberg equilibrium (p > 0.05). A significant difference in genotype distribution and allele frequency was observed between patients and controls. Male patients with MI had a frequency of 97 % for GG genotype and 3 % for GA+AA genotypes. The control group had a frequency of 99 % for the GG genotype and 1 % for the GA+AA genotypes which is significantly lower than the frequency found in patients (p = 0.01). The same genotype frequencies were found in women (p = 0.032). The MI patient group showed a significantly higher frequency of 20210A allele compared to controls 0.02 versus 0.01 [OR = 3.60 (95 % CI = 1.29-10.53), p = 0.005] in men and 0.015 versus 0.068 [OR = 4.68 (95 % CI = 1.60-14.26), p = 0.001] in women. Our work showed a significant but not independent association between the G20210A polymorphism of the prothrombin gene and MI in the Tunisian population.
Faculté de Medicine de Tunis, Université de Tunis El Manar, 1007, Tunis, Tunisia.|Hôpital la Rabta, Service de Biochimie, Université de Tunis El Manar, LR99ES11, 1007, Tunis, Tunisia.
Amani
A
Kallel
Faculté de Medicine de Tunis, Université de Tunis El Manar, 1007, Tunis, Tunisia.|Hôpital la Rabta, Service de Biochimie, Université de Tunis El Manar, LR99ES11, 1007, Tunis, Tunisia.
Mohamed Hedi
MH
Sbaï
Faculté de Medicine de Tunis, Université de Tunis El Manar, 1007, Tunis, Tunisia.|Hôpital la Rabta, Service de Biochimie, Université de Tunis El Manar, LR99ES11, 1007, Tunis, Tunisia.
Yousra
Y
Sédiri
Faculté de Medicine de Tunis, Université de Tunis El Manar, 1007, Tunis, Tunisia.|Hôpital la Rabta, Service de Biochimie, Université de Tunis El Manar, LR99ES11, 1007, Tunis, Tunisia.
Moncef
M
Feki
Hôpital la Rabta, Service des Explorations Fonctionnelles et de Reanimations en Cardiologie, Université de Tunis El Manar, Tunis, Tunisia.
Mohamed Sami
MS
Mourali
Hôpital la Rabta, Service des Explorations Fonctionnelles et de Reanimations en Cardiologie, Université de Tunis El Manar, Tunis, Tunisia.
Rachid
R
Mechmeche
Faculté de Medicine de Tunis, Université de Tunis El Manar, 1007, Tunis, Tunisia. jemaa_riadh@yahoo.fr.|Hôpital la Rabta, Service de Biochimie, Université de Tunis El Manar, LR99ES11, 1007, Tunis, Tunisia. jemaa_riadh@yahoo.fr.
Riadh
R
Jemaa
Faculté de Medicine de Tunis, Université de Tunis El Manar, 1007, Tunis, Tunisia.|Hôpital la Rabta, Service de Biochimie, Université de Tunis El Manar, LR99ES11, 1007, Tunis, Tunisia.
Naziha
N
Kaabachi
D000328:Adult; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011516:Prothrombin / Q000235:genetics*; D014416:Tunisia; D044465:White People / Q000235:genetics*
D011516:Prothrombin
Myocardial infarction; Polymorphism; Prothrombin