In vitro DNA damage by Casiopeina II-gly in human blood cells.
A variety of metal ions have biological functions, and many researchers have not actively investigated copper compounds, based on the assumption that endogenous metals might be less toxic. In the present study, we used a dual fluorochrome method and a single cell gel electrophoresis (SCGE) assay at pH > 13 to evaluate the cell viability and DNA damage induced by a copper-based antineoplastic drug, Casiopeina II-gly®, at concentrations of 1.04, 2.08, 4.17, 8.35 or 16 μg/mL in human peripheral-blood leukocytes in vitro. We observed that Casiopeina II-gly® reduced cell viability at high concentrations (8.35 and 16 μg/mL) and induced DNA damage characterized by single-strand breaks and alkali labile sites at several concentrations from 2.08 to 16 μg/mL. This chemical clearly affected DNA migration in a concentration- and time-dependent manner and induced genotoxic effects in few minutes (>20 min), at which point the genotoxicity was followed by cytotoxicity.
a Unidad de Investigación en Genética y Toxicología Ambiental (UIGTA), Laboratorio L5 PA, Unidad Multidisciplinaria de Investigación Experimental (UMIE-Z), Facultad de Estudios Superiores-Zaragoza Campus II, UNAM. CP 15000 , Ciudad de México , México.
Juan José
JJ
Rodríguez-Mercado
a Unidad de Investigación en Genética y Toxicología Ambiental (UIGTA), Laboratorio L5 PA, Unidad Multidisciplinaria de Investigación Experimental (UMIE-Z), Facultad de Estudios Superiores-Zaragoza Campus II, UNAM. CP 15000 , Ciudad de México , México.
Diana
D
Florín-Ramírez
a Unidad de Investigación en Genética y Toxicología Ambiental (UIGTA), Laboratorio L5 PA, Unidad Multidisciplinaria de Investigación Experimental (UMIE-Z), Facultad de Estudios Superiores-Zaragoza Campus II, UNAM. CP 15000 , Ciudad de México , México.
Lucila
L
Álvarez-Barrera
a Unidad de Investigación en Genética y Toxicología Ambiental (UIGTA), Laboratorio L5 PA, Unidad Multidisciplinaria de Investigación Experimental (UMIE-Z), Facultad de Estudios Superiores-Zaragoza Campus II, UNAM. CP 15000 , Ciudad de México , México.
Mario Agustín
MA
Altamirano-Lozano
D002470:Cell Survival / Q000187:drug effects; D020552:Comet Assay; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D007962:Leukocytes / Q000187:drug effects* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009153:Mutagens / Q000633:toxicity*; D009942:Organometallic Compounds / Q000633:toxicity*; D018570:Risk Assessment; D013997:Time Factors; D055815:Young Adult
D009153:Mutagens; D009942:Organometallic Compounds; C505569:casiopeina II-glycine
Casiopeina II-gly; Copper-based antineoplastic drug; DNA damage; SCGE in human leucocytes; cytotoxicity; genotoxicity