Capillary electrophoresis fingerprinting and spectrophotometric determination of antioxidant potential for classification of Mentha products.
In this work aqueous infusions from ten Mentha herbal samples (four different Mentha species and six hybrids of Mentha x piperita) and 20 different peppermint teas were screened by capillary electrophoresis with UV detection. The fingerprint separation was accomplished in a 25 mM borate background electrolyte with 10% methanol at pH 9.3. The total polyphenolic content in the extracts was determined spectrophotometrically at 765 nm by a Folin-Ciocalteu phenol assay. Total antioxidant activity was determined by scavenging of 2,2-diphenyl-1-picrylhydrazyl radical at 515 nm. The peak areas of 12 dominant peaks from CE analysis, present in all samples, and the value of total polyphenolic content and total antioxidant activity obtained by spectrophotometry was combined into a single data matrix and principal component analysis was applied. The obtained principal component analysis model resulted in distinct clusters of Mentha and peppermint tea samples distinguishing the samples according to their potential protective antioxidant effect. Principal component analysis, using a non-targeted approach with no need for compound identification, was found as a new promising tool for the screening of herbal tea products.
Department of Chemistry, Masaryk University, Brno, Czech Republic.
Vendula
V
Roblová
Department of Chemistry, Masaryk University, Brno, Czech Republic.
Miroslava
M
Bittová
Department of Chemistry, Masaryk University, Brno, Czech Republic.
Petr
P
Kubáň
Department of Food Technology, Faculty of Technology, Tomas Bata University, Zlín, Czech Republic.
Vlastimil
V
Kubáň
D000975:Antioxidants / Q000032:analysis*; D019075:Electrophoresis, Capillary; D027541:Mentha / Q000737:chemistry*; D010946:Plants, Medicinal; D013053:Spectrophotometry
D000975:Antioxidants
Capillary electrophoresis; Fingerprinting; Infusions; Mentha x piperita, Peppermint tea