The use of platelet-rich fibrin combined with periodontal ligament and jaw bone mesenchymal stem cell sheets for periodontal tissue engineering.
Periodontal regeneration involves the restoration of at least three unique tissues: cementum, periodontal ligament tissue (PDL) and alveolar bone tissue. Here, we first isolated human PDL stem cells (PDLSCs) and jaw bone mesenchymal stem cells (JBMSCs). These cells were then induced to form cell sheets using an ascorbic acid-rich approach, and the cell sheet properties, including morphology, thickness and gene expression profile, were compared. Platelet-rich fibrin (PRF) derived from human venous blood was then fabricated into bioabsorbable fibrin scaffolds containing various growth factors. Finally, the in vivo potential of a cell-material construct based on PDLSC sheets, PRF scaffolds and JBMSC sheets to form periodontal tissue was assessed in a nude mouse model. In this model, PDLSC sheet/PRF/JBMSC sheet composites were placed in a simulated periodontal space comprising human treated dentin matrix (TDM) and hydroxyapatite (HA)/tricalcium phosphate (TCP) frameworks. Eight weeks after implantation, the PDLSC sheets tended to develop into PDL-like tissues, while the JBMSC sheets tended to produce predominantly bone-like tissues. In addition, the PDLSC sheet/PRF/JBMSC sheet composites generated periodontal tissue-like structures containing PDL- and bone-like tissues. Further improvements in this cell transplantation design may have the potential to provide an effective approach for future periodontal tissue regeneration.
State Key Laboratory of Military Stomatology &National Clinical Research Center for Oral Diseases &Shaanxi Key Laboratory of Oral Diseases, Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi, China.
Zhong-Shan
ZS
Wang
State Key Laboratory of Military Stomatology &National Clinical Research Center for Oral Diseases &Shaanxi Key Laboratory of Oral Diseases, Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi, China.
Zhi-Hong
ZH
Feng
State Key Laboratory of Military Stomatology &National Clinical Research Center for Oral Diseases &Shaanxi Key Laboratory of Oral Diseases, Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi, China.
Guo-Feng
GF
Wu
State Key Laboratory of Military Stomatology &National Clinical Research Center for Oral Diseases &Shaanxi Key Laboratory of Oral Diseases, Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi, China.
Shi-Zhu
SZ
Bai
State Key Laboratory of Military Stomatology &National Clinical Research Center for Oral Diseases &Shaanxi Key Laboratory of Oral Diseases, Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi, China.
Yan
Y
Dong
State Key Laboratory of Military Stomatology &National Clinical Research Center for Oral Diseases &Shaanxi Engineering Research Center for Dental Materials and Advanced Manufacture, Department of Periodontology, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi, China.
Fa-Ming
FM
Chen
State Key Laboratory of Military Stomatology &National Clinical Research Center for Oral Diseases &Shaanxi Key Laboratory of Oral Diseases, Department of Prosthodontics, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi, China.
Yi-Min
YM
Zhao
D000293:Adolescent; D000328:Adult; D000818:Animals; D002130:Calcium Phosphates; D002454:Cell Differentiation; D002478:Cells, Cultured; D003804:Dentin; D016557:Guided Tissue Regeneration, Periodontal; D006801:Humans; D008297:Male; D008334:Mandible / Q000502:physiology / Q000601:surgery*; D008437:Maxilla / Q000502:physiology / Q000601:surgery*; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology; D051379:Mice; D008819:Mice, Nude; D010012:Osteogenesis; D010513:Periodontal Ligament / Q000502:physiology / Q000601:surgery*; D010518:Periodontitis / Q000601:surgery / Q000628:therapy*; D000073183:Platelet-Rich Fibrin / Q000378:metabolism*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry; D055815:Young Adult
D002130:Calcium Phosphates; C018392:tricalcium phosphate