Remote Preconditioning on Rat Hepatic Ischemia-Reperfusion Injury Downregulated Bax and Cleaved Caspase-3 Expression.
OBJECTIVE
Hepatic ischemia-reperfusion injury (IRI) is considered a major cause of hepatic damage in liver surgery. The aim of this study was to investigate the effect of the remote ischemic perconditioning method on hepatic IRI in a rat model.


METHODS
Seventeen rats underwent hepatic IRI for 30 minutes followed by reperfusion, and were divided into 3 groups: group I, only hepatic IRI (n = 5); group II, hepatic IRI with remote perconditioning (n = 7); and group III, hepatic IRI with remote postconditioning (n = 5).


RESULTS
For Bax/β-actin, mean values of the 3 groups (±standard deviation) were 1.29 ± 0.26 (group I), 0.89 ± 0.15 (group II), and 1.02 ± 0.23 (group III). The level of Bax/β-actin in group II was significantly lower than in group I (P < .01). The cleaved Caspase-3/β-actin ratio for groups I, II, and III was 0.93 ± 0.22, 0.46 ± 0.16, and 0.63 ± 0.22, respectively. The level of cleaved Caspase-3/β-actin in groups II and III were significantly lower than in group I (P < .01 and P < .05, respectively). The Bcl-2/β-actin ratio for groups I, II, and III was 1.01 ± 0.09, 1.19 ± 0.39, and 1.20 ± 0.12, respectively. However, there were no significant difference between groups II and III and group I.


CONCLUSIONS
The remote perconditioning on rat hepatic IRI downregulated the Bax and cleaved Caspase-3 expression.
Department of Surgery, School of Medicine, Kyung Hee University Medical Center.
M-S
MS
Park
Department of Surgery, School of Medicine, Kyung Hee University Medical Center.
S H
SH
Joo
Department of Surgery, School of Medicine, Kyung Hee University Medical Center.
B S
BS
Kim
Medical Science Research Institute, Kyung Hee University Medical Center.
J W
JW
Lee
Medical Science Research Institute, Kyung Hee University Medical Center.
Y I
YI
Kim
Department of Surgery, School of Medicine, Kyung Hee University Medical Center.
M K
MK
Hong
Department of Surgery, School of Medicine, Kyung Hee University Medical Center. Electronic address: whipple@khu.ac.kr.
H J
HJ
Ahn
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism*; D015536:Down-Regulation; D019194:Ischemic Preconditioning / Q000379:methods*; D008099:Liver / Q000098:blood supply* / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000517:prevention & control*; D051028:bcl-2-Associated X Protein / Q000378:metabolism*
C494828:Bax protein, rat; D015415:Biomarkers; D051028:bcl-2-Associated X Protein; C505410:Casp3 protein, rat; D053148:Caspase 3