Heat shock protein 90 is required for sexual and asexual development, virulence, and heat shock response in Fusarium graminearum.
Eukaryotic cells repress global translation and selectively upregulate stress response proteins by altering multiple steps in gene expression. In this study, genome-wide transcriptome analysis of cellular adaptation to thermal stress was performed on the plant pathogenic fungus Fusarium graminearum. The results revealed that profound alterations in gene expression were required for heat shock responses in F. graminearum. Among these proteins, heat shock protein 90 (FgHsp90) was revealed to play a central role in heat shock stress responses in this fungus. FgHsp90 was highly expressed and exclusively localised to nuclei in response to heat stress. Moreover, our comprehensive functional characterisation of FgHsp90 provides clear genetic evidence supporting its crucial roles in the vegetative growth, reproduction, and virulence of F. graminearum. In particular, FgHsp90 performs multiple functions as a transcriptional regulator of conidiation. Our findings provide new insight into the mechanisms underlying adaptation to heat shock and the roles of Hsp90 in fungal development.
Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Republic of Korea.
Duc-Cuong
DC
Bui
Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Republic of Korea.
Yoonji
Y
Lee
Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Republic of Korea.
Jae Yun
JY
Lim
Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Republic of Korea.
Minmin
M
Fu
Division of Applied Bioscience and Biotechnology, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Republic of Korea.
Jin-Cheol
JC
Kim
Eco-friendly New Materials Research Group, Research Centre for Biobased Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.
Gyung Ja
GJ
Choi
Centre for Food and Bioconvergence, Seoul National University, Seoul 08826, Republic of Korea.
Hokyoung
H
Son
Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Republic of Korea.
Yin-Won
YW
Lee
D005670:Fusarium / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity*; D015870:Gene Expression; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal / Q000235:genetics*; D005800:Genes, Fungal / Q000235:genetics; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D018869:Heat-Shock Response / Q000235:genetics*; D013172:Spores, Fungal / Q000254:growth & development*; D014157:Transcription Factors / Q000235:genetics; D015533:Transcriptional Activation / Q000235:genetics
D018841:HSP90 Heat-Shock Proteins; D014157:Transcription Factors