Isolation of Bdellovibrio sp. from soil samples in Mexico and their potential applications in control of pathogens.
In this study, two strains of Bdellovibrio were isolated from soil samples using the culture-dependent technique and two members of the family Enterobacteriaceae (Klebsiella sp. and Salmonella sp.) as prey. The Bdellovibrio strains were bacteriolytic, plaque-forming, and highly motile gram-negative bacteria. We identified and confirmed the Bdellovibrio strains using microscopy, PCR amplification, and sequencing of the 16S rRNA gene. They were observed to be different strains based on hit locus and prey range analyses. Here, the first report on Bdellovibrio strains isolated from soil in Mexico corroborates earlier report indicating that populations of Bdellovibrio found in soil are heterogeneous thereby the need to identify the various strains.
Instituto Politécnico Nacional, Centro de Biotecnología Genómica, Reynosa, Tamaulipas, 88710, México.|Department of Biological Sciences, College of Science, Engineering and Technology, Faculty of Basic and Applied Science, Osun State University, Osogbo, Osun State, Nigeria.
Omotayo Opemipo
0000-0002-6815-6162
OO
Oyedara
Instituto Politécnico Nacional, Centro de Biotecnología Genómica, Reynosa, Tamaulipas, 88710, México.
Erick de Jesus
EJ
De Luna-Santillana
Instituto Politécnico Nacional, Centro de Biotecnología Genómica, Reynosa, Tamaulipas, 88710, México.
Omar
O
Olguin-Rodriguez
Instituto Politécnico Nacional, Centro de Biotecnología Genómica, Reynosa, Tamaulipas, 88710, México.
Xianwu
X
Guo
Facultad de Ciencias Físico-Matemáticas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Nuevo León, 66455, Mexico.
Marco Antonio
MA
Mendoza-Villa
Facultad de Ciencias Físico-Matemáticas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Nuevo León, 66455, Mexico.
Jorge Luis
JL
Menchaca-Arredondo
Instituto Politécnico Nacional, Centro de Biotecnología Genómica, Reynosa, Tamaulipas, 88710, México.
Temidayo Oluyomi
TO
Elufisan
Instituto Politécnico Nacional, Centro de Biotecnología Genómica, Reynosa, Tamaulipas, 88710, México.
Javier Alfonso
JA
Garza-Hernandez
Instituto Politécnico Nacional, Centro de Biotecnología Genómica, Reynosa, Tamaulipas, 88710, México.
Israel
I
Garcia Leon
Instituto Politécnico Nacional, Centro de Biotecnología Genómica, Reynosa, Tamaulipas, 88710, México.
Mario Alberto
MA
Rodriguez-Perez
D001433:Bacteriolysis / Q000502:physiology*; D001483:Base Sequence; D001501:Bdellovibrio / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D061046:Biological Control Agents / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D007709:Klebsiella / Q000254:growth & development*; D008800:Mexico; D018625:Microscopy, Atomic Force; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012475:Salmonella / Q000254:growth & development*; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D061046:Biological Control Agents; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S

Bdellovibrio
; Atomic force microscopy; hit locus; host-range