The method of purifying bioengineered spider silk determines the silk sphere properties.
Bioengineered spider silks are a biomaterial with great potential for applications in biomedicine. They are biocompatible,biodegradable and can self-assemble into films, hydrogels, scaffolds, fibers, capsules and spheres. A novel, tag-free, bioengineered spider silk named MS2(9x) was constructed. It is a 9-mer of the consensus motif derived from MaSp2-the spidroin of Nephila clavipes dragline silk. Thermal and acidic extraction methods were used to purify MS2(9x). Both purification protocols gave a similar quantity and quality of soluble silk; however, they differed in the secondary structure and zeta potential value. Spheres made of these purified variants differed with regard to critical features such as particle size, morphology, zeta potential and drug loading. Independent of the purification method, neither variant of the MS2(9x) spheres was cytotoxic, which confirmed that both methods can be used for biomedical applications. However, this study highlights the impact that the applied purification method has on the further biomaterial properties.
Chair of Medical Biotechnology, Poznan University of Medical Sciences, 61-688 Poznan, Poland.|NanoBioMedical Centre, Adam Mickiewicz University, 61-614 Poznan, Poland.
Katarzyna
K
Jastrzebska
BioContract Sp. z o.o., 61-051 Poznan, Poland.
Edyta
E
Felcyn
Department of Macromolecular Physics, Adam Mickiewicz University, 61-614 Poznan, Poland.|Joint Laboratory for SAXS Studies, Adam Mickiewicz University, 61-614 Poznan, Poland.
Maciej
M
Kozak
Faculty of Technical Physics, Poznan University of Technology, 60-965 Poznan, Poland.
Miroslaw
M
Szybowicz
Faculty of Technical Physics, Poznan University of Technology, 60-965 Poznan, Poland.
Tomasz
T
Buchwald
Department of Macromolecular Physics, Adam Mickiewicz University, 61-614 Poznan, Poland.
Zuzanna
Z
Pietralik
Faculty of Chemical Technology, Institute of Chemical Technology and Engineering, Poznan University of Technology, 60-965 Poznan, Poland.
Teofil
T
Jesionowski
Chair of Medical Biotechnology, Poznan University of Medical Sciences, 61-688 Poznan, Poland.|BioContract Sp. z o.o., 61-051 Poznan, Poland.|Department of Diagnostics and Cancer Immunology, Greater Poland Cancer Centre, 61-688 Poznan, Poland.
Andrzej
A
Mackiewicz
Chair of Medical Biotechnology, Poznan University of Medical Sciences, 61-688 Poznan, Poland.|Department of Diagnostics and Cancer Immunology, Greater Poland Cancer Centre, 61-688 Poznan, Poland.
Hanna
H
Dams-Kozlowska
D000595:Amino Acid Sequence; D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry*; D001698:Biomedical Engineering; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry*; D059625:Liquid-Liquid Extraction / Q000379:methods; D008855:Microscopy, Electron, Scanning; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000138:chemical synthesis / Q000737:chemistry; D047011:Silk / Q000138:chemical synthesis / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013112:Spiders / Q000378:metabolism*
D001672:Biocompatible Materials; D004337:Drug Carriers; D011994:Recombinant Proteins; D047011:Silk