Gadolinium Nanoparticles Conjugated with Therapeutic Bifunctional Chelate as a Potential T1 Theranostic Magnetic Resonance Imaging Agent.
This work is directed toward the synthesis of two types of gadolinium oxide nanoparticles (Gd-oxide NPs), abbreviated as Gd@SiO2-DO3A and Gd@SiO2-DO2A-BTA, with diameters of 50-60 nm. The synthesis involves sequential coating of Gd-oxide NPs with tetraethyl orthosilicate (TEOS) and (3-aminopropyl) triethoxysilane (APTES), followed by functionalization of the aminopropylsilane group with 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) or 1,4,7,10-tetraazacyclododecane-1,4,7-trisacetic acid conjugates of benzothiazoles (DO3A-BTA). Gd@SiO2-DO3A and Gd@SiO2-DO2A-BTA exhibit high water solubility and colloidal stability. The r1 relaxivities of both Gd@SiO2-DO3A and Gd@SiO2-DO2A-BTA are higher than those of the corresponding low-molecular-weight magnetic resonance imaging contrast agents (MRI CAs), and their r2/r1 ratios are close to 1, indicating that both can be used as potential T1 MRI CAs. Biodistribution studies demonstrated that Gd@SiO2-DO2A-BTA was excreted via both hepatobiliary and renal pathways. Gd@SiO2-DO2A-BTA exhibits a strong intracellular uptake property in a series of tumor cell lines, and has significant anticancer characteristics against cell lines such as SK-HEP-1, MDA-MB-231, HeLa, and Hep-3B.
Min-Kyoung
MK
Kang
Gang Ho
GH
Lee
Ki-Hye
KH
Jung
Jae-Chang
JC
Jung
Hee-Kyung
HK
Kim
Yeon-Hee
YH
Kim
Jongmin
J
Lee
Hun-Kyu
HK
Ryeom
Tae-Jeong
TJ
Kim
Yongmin
Y
Chang
D000818:Animals; D052160:Benzothiazoles / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002614:Chelating Agents / Q000737:chemistry*; D003102:Colloids; D003228:Conjunctiva / Q000166:cytology; D004195:Disease Models, Animal; D000067493:Dynamic Light Scattering; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005682:Gadolinium / Q000737:chemistry*; D006801:Humans; D008099:Liver / Q000473:pathology; D008279:Magnetic Resonance Imaging; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D012996:Solutions; D017550:Spectroscopy, Fourier Transform Infrared; D055672:Static Electricity; D000068936:Theranostic Nanomedicine; D014018:Tissue Distribution / Q000187:drug effects; D014867:Water / Q000737:chemistry
D052160:Benzothiazoles; D002614:Chelating Agents; D003102:Colloids; D012996:Solutions; D014867:Water; D005682:Gadolinium; C005465:benzothiazole