Assessing the potential of photosensitizing flavoproteins as tags for correlative microscopy.
Photosensitizing flavoproteins have great potential as tags for correlative light and electron microscopy (CLEM). We examine the photostability of miniSOG mutants and their ability to photo-oxidize diaminobenzidine, both key aspects for CLEM. Our experiments reveal a complex relation between these parameters and the production of different reactive oxygen species.
Madrid Institute for Advanced Studies in Nanoscience (IMDEA Nanoscience) and Nanobiotechnology Unit Associated to the National Center for Biotechnology (CSIC), 28049 Madrid, Spain. cristina.flors@imdea.org.
Alberto
A
Rodríguez-Pulido
Madrid Institute for Advanced Studies in Nanoscience (IMDEA Nanoscience) and Nanobiotechnology Unit Associated to the National Center for Biotechnology (CSIC), 28049 Madrid, Spain. cristina.flors@imdea.org and CIC biomaGUNE, Paseo de Miramón 182, E-20009 Donostia-San Sebastian, Spain.
Aitziber L
AL
Cortajarena
Institut Químic de Sarrià, Universitat Ramon Llull, 08017 Barcelona, Spain.
Joaquim
J
Torra
Institut Químic de Sarrià, Universitat Ramon Llull, 08017 Barcelona, Spain.
Rubén
R
Ruiz-González
Institut Químic de Sarrià, Universitat Ramon Llull, 08017 Barcelona, Spain.
Santi
S
Nonell
Madrid Institute for Advanced Studies in Nanoscience (IMDEA Nanoscience) and Nanobiotechnology Unit Associated to the National Center for Biotechnology (CSIC), 28049 Madrid, Spain. cristina.flors@imdea.org.
Cristina
C
Flors
D005420:Flavoproteins / Q000378:metabolism*; D008027:Light; D008854:Microscopy, Electron; D017319:Photosensitizing Agents / Q000378:metabolism*; D026082:Singlet Oxygen / Q000378:metabolism
D005420:Flavoproteins; D017319:Photosensitizing Agents; D026082:Singlet Oxygen