Enzymatic synthesis using immobilized Enterococcus faecalis Esawy dextransucrase and some applied studies.
Dextrans enzymatic synthesis by immobilized Enterococcus faecalis Esawy dextransucrase was studied. Different parameters, such as: enzyme protein concentration (EPC), substrate concentration (SC), temperature and reaction time were evaluated. EPC played a fundamental role in controlling dextran molecular size with 0.1% dextran in reaction mixture. Dextran 38,397 and 125,471Da were yielded at EPC 4.78 and 5.78mg, respectively. Proper dextrans (73,378 and 117,521Da) demanded in pharmaceutical applications were achieved at 6% and 12% sucrose concentrations and at 4.78 and 5.78mg EPC, respectively. Optimum temperature for conversion of glucose to dextran was 30°C (73% and 80% at 5.78 and 4.78mg EPC, respectively). Varieties of maltooligosaccharides (MOS) were yielded by synergistic cooperation between sucrose and maltose. Six MOS and three dextrans samples in vitro have prebiotic effect on Lactobacillus casei with degree of variation. Two samples of MOS with different degree of polymerization (DP) and three samples of dextran with different molecular weight (MW) reported different fibrinolytic activity.
Department of Chemistry of Microbial and Natural Products, National Research Centre (NRC), Dokki, Cairo, Egypt.
Mona A
MA
Esawy
Department of Chemistry of Microbial and Natural Products, National Research Centre (NRC), Dokki, Cairo, Egypt.
Amira A
AA
Gamal
Department of Chemistry of Microbial and Natural Products, National Research Centre (NRC), Dokki, Cairo, Egypt.
Mohamed M I
MM
Helal
Department of Chemistry of Microbial and Natural Products, National Research Centre (NRC), Dokki, Cairo, Egypt; Centre of Excellence, Laboratories of Advanced Materials and Nanotechnology, Polymer Dept., National Research Centre (NRC), Dokki, Cairo, Egypt.
Mohamed E
ME
Hassan
Department of microbiology, Faculty of Science, Ain Shams University, Cairo, Egypt.
Naziha M
NM
Hassanein
Department of Chemistry of Microbial and Natural Products, National Research Centre (NRC), Dokki, Cairo, Egypt. Electronic address: amal_mhashem@yahoo.com.
Amal M
AM
Hashem
D018914:Cells, Immobilized / Q000737:chemistry / Q000378:metabolism; D013293:Enterococcus faecalis / Q000737:chemistry / Q000378:metabolism*; D005964:Glucosyltransferases / Q000096:biosynthesis* / Q000737:chemistry; D007780:Lacticaseibacillus casei / Q000737:chemistry / Q000378:metabolism
D005964:Glucosyltransferases; C020025:dextransucrase
Dextran; Enterococcus faecalis; Enzymatic synthesis; Fibrinolytic; Immobilized dextransucrase; Maltooligosaccharides; Prebiotic