Nitric oxide: a novel inducer for enhancement of microbial lipase production.
The purpose of this study was to elucidate whether exogenous nitric oxide (NO) has a potential beneficial effect on lipase production capacity of some microorganisms. Sodium nitroprusside (SNP) was used as an exogenous NO donor in production medium. In comparison with the control (0 nM SNP), SNP concentrations from 10 to 100 nM induced lipase production in mesophilic bacterium Bacillus subtilis and cold-adapted yeast Yarrowia lipolytica. Especially, the maximum lipase activities for Y. lipolytica (81.2 U/L) and B. subtilis (74.5 U/L) were attained at 30 and 50 nM SNP concentrations, respectively. When compared to the control, the optimal SNP concentrations resulted in about 5.14 and 2.27-fold increases in lipase activities of B. subtilis and Y. lipolytica, respectively. Besides, it was found that the optimal SNP concentrations provided shorter incubation periods for lipase production. Conversely, no significant positive effect of exogenous NO on lipase production was determined for thermophilic bacterium Geobacillus stearothermophilus. This study showed for the first time that exogenous NO could be used as an inducer in the production of microbial lipases.
Department of Molecular Biology and Genetics, Science Faculty, Ataturk University, Erzurum, Turkey. mesuttaskin61@hotmail.com.
Mesut
M
Taskin
Department of Molecular Biology and Genetics, Science Faculty, Ataturk University, Erzurum, Turkey.
Yagmur
Y
Unver
Department of Molecular Biology and Genetics, Science Faculty, Ataturk University, Erzurum, Turkey.
Melike
M
Yildiz
Department of Molecular Biology and Genetics, Science Faculty, Erzurum Technical University, Erzurum, Turkey.
Serkan
S
Ortucu
Department of Molecular Biology and Genetics, Science Faculty, Ataturk University, Erzurum, Turkey.
Hakan
H
Askin
D001412:Bacillus subtilis / Q000201:enzymology*; D001426:Bacterial Proteins / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction / Q000187:drug effects; D008049:Lipase / Q000096:biosynthesis*; D009569:Nitric Oxide / Q000378:metabolism*; D009599:Nitroprusside / Q000494:pharmacology*; D025062:Yarrowia / Q000201:enzymology*
D001426:Bacterial Proteins; D009599:Nitroprusside; D009569:Nitric Oxide; D008049:Lipase
Inducer; Lipase; Microbial production; Nitric oxide