miR-204 suppresses non-small-cell lung carcinoma (NSCLC) invasion and migration by targeting JAK2.
Aberrant expression of microRNA is associated with the development and progression of cancers. MicroRNA-204 (miR-204) down-regulation has been previously demonstrated in non-small-cell lung carcinoma (NSCLC); however, the underlying mechanism by which miR-204 suppresses tumorigenesis in NSCLC remains elusive. In this study, miR-204 expression was found to be down-regulated, and that of Janus kinase 2 (JAK2) was found to be up-regulated in four NSCLC cell lines (A549, H1299, H1650, and H358) compared to the normal lung cell line. The overexpression of miR-204 suppressed the invasive and migratory capacities of H1299 cells. A luciferase assay confirmed that the binding of miR-124 to the -untranslated region of JAK2 inhibited the expression of JAK2 proteins in H1299 cells. JAK-2 overexpression effectively reversed miR-204-repressed NSCLC metastasis. Taken together, our findings revealed that miR-204 functions as a tumor suppressor in NSCLC by targeting JAK2, and that miR-204 may therefore serve as a biomarker for the diagnosis and treatment of NSCLC.
Department of Oncology, the Yantaishan Hospital, Yantai, Shandong Province, China.
P
P
Wang
Department of Oncology, The Qingdao School of Medicine, Yantai, Shandong Province, China.
H Y
HY
Lv
Department of Oncology, the Yantaishan Hospital, Yantai, Shandong Province, China.
D M
DM
Zhou
Department of Oncology, the Yantaishan Hospital, Yantai, Shandong Province, China.
E N
EN
Zhang
D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053614:Janus Kinase 2 / Q000096:biosynthesis* / Q000235:genetics; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics
C549404:MIRN204 microRNA, human; D035683:MicroRNAs; C507924:JAK2 protein, human; D053614:Janus Kinase 2