Temporal and spatial distribution of mast cells and steroidogenic enzymes in the human fetal adrenal.
Mast cells are present in the human adult adrenal with a potential role in the regulation of aldosterone secretion in both normal cortex and adrenocortical adenomas. We have investigated the human developing adrenal gland for the presence of mast cells in parallel with steroidogenic enzymes profile and serotonin signaling pathway. RT-QPCR and immunohistochemical studies were performed on adrenals at 16-41 weeks of gestation (WG). Tryptase-immunopositive mast cells were found from 18 WG in the adrenal subcapsular layer, close to 3βHSD- and CYP11B2-immunoreactive cells, firstly detected at 18 and 24 WG, respectively. Tryptophan hydroxylase and serotonin receptor type 4 expression increased at 30 WG before the CYP11B2 expression surge. In addition, HDL and LDL cholesterol receptors were expressed in the subcapsular zone from 24 WG. Altogether, our findings suggest the implication of mast cells and serotonin in the establishment of the mineralocorticoid synthesizing pathway during fetal adrenal development.
INSERM U982, Laboratory of Differentiation & Neuronal and Neuroendocrine Communication, Institute for Research and Innovation in Biomedicine, University of Rouen, Mont-Saint-Aignan, France. Electronic address: alexandre.naccache@chu-rouen.fr.
Alexandre
A
Naccache
INSERM U982, Laboratory of Differentiation & Neuronal and Neuroendocrine Communication, Institute for Research and Innovation in Biomedicine, University of Rouen, Mont-Saint-Aignan, France. Electronic address: estelle.louiset@univ-rouen.fr.
Estelle
E
Louiset
INSERM U982, Laboratory of Differentiation & Neuronal and Neuroendocrine Communication, Institute for Research and Innovation in Biomedicine, University of Rouen, Mont-Saint-Aignan, France. Electronic address: celine.duparc@univ-rouen.fr.
Céline
C
Duparc
Pathology Laboratory, University Hospital of Rouen, Rouen, France. Electronic address: annie.laquerriere@chu-rouen.fr.
Annie
A
Laquerrière
Pathology Laboratory, University Hospital of Rouen, Rouen, France. Electronic address: sophie.patrier@chu-rouen.fr.
Sophie
S
Patrier
INSERM U982, Laboratory of Differentiation & Neuronal and Neuroendocrine Communication, Institute for Research and Innovation in Biomedicine, University of Rouen, Mont-Saint-Aignan, France. Electronic address: sylvie.renouf@univ-rouen.fr.
Sylvie
S
Renouf
Endocrine Section, Department of Medicine, G.V. (Sonny) Montgomery VA Medical Center and University of Mississippi Medical Center, Jackson, MS, USA. Electronic address: Cgomez-sanchez@umc.edu.
Celso E
CE
Gomez-Sanchez
Department of Biochemistry, School of Medicine, Keio University, Tokyo, 160-8582, Japan. Electronic address: mukaik@sc.itc.keio.ac.jp.
Kuniaki
K
Mukai
INSERM U982, Laboratory of Differentiation & Neuronal and Neuroendocrine Communication, Institute for Research and Innovation in Biomedicine, University of Rouen, Mont-Saint-Aignan, France. Electronic address: herve.lefebvre@chu-rouen.fr.
Hervé
H
Lefebvre
INSERM U982, Laboratory of Differentiation & Neuronal and Neuroendocrine Communication, Institute for Research and Innovation in Biomedicine, University of Rouen, Mont-Saint-Aignan, France. Electronic address: mireille.castanet@chu-rouen.fr.
Mireille
M
Castanet
D015096:3-Hydroxysteroid Dehydrogenases / Q000235:genetics / Q000378:metabolism; D000311:Adrenal Glands / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D000450:Aldosterone / Q000378:metabolism*; D019405:Cytochrome P-450 CYP11B2 / Q000235:genetics / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D008075:Lipoproteins, HDL / Q000235:genetics / Q000378:metabolism; D008407:Mast Cells / Q000166:cytology* / Q000378:metabolism; D011247:Pregnancy; D011973:Receptors, LDL / Q000235:genetics / Q000378:metabolism; D018110:Receptors, Lipoprotein / Q000235:genetics / Q000378:metabolism; D011985:Receptors, Serotonin / Q000235:genetics / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism*; D015398:Signal Transduction; D053802:Tryptases / Q000235:genetics / Q000378:metabolism; D014365:Tryptophan Hydroxylase / Q000235:genetics / Q000378:metabolism
D008075:Lipoproteins, HDL; D011973:Receptors, LDL; D018110:Receptors, Lipoprotein; D011985:Receptors, Serotonin; C031616:high density lipoprotein receptors; D012701:Serotonin; D000450:Aldosterone; D015096:3-Hydroxysteroid Dehydrogenases; D019405:Cytochrome P-450 CYP11B2; D014365:Tryptophan Hydroxylase; D053802:Tryptases
Aldosterone; Human fetal adrenal; Mast cells; Microenvironment; Serotonin; Steroidogenesis