Design of ultrasensitive bisphenol A-aptamer based on platinum nanoparticles loading to polyethyleneimine-functionalized carbon nanotubes.
Here, a highly sensitive electrochemical aptasensor based on a novel signal amplification strategy for the determination of bisphenol A (BPA) was developed. Construction of the aptasensor began with the deposition of highly dispersed platinum nanoparticles (PtNPs)/acid-oxidized carbon nanotubes (CNTs-COOH) functionalized with polyethyleneimine (PEI) at the surface of glassy carbon (PtNPs/PEI/CNTs-COOH/GC) electrode. After immobilizing the amine-capped capture probe (ssDNA1) through the covalent amide bonds formed by the carboxyl groups on the nanotubes and the amino groups on the oligonucleotides, we employed a designed complementary BPA-aptamer (ssDNA2) as a detection probe to hybridize with the ssDNA1. By adding BPA as a target, the aptamer specifically bound to BPA and its end folded into a BPA-binding junction. Because of steric/conformational restrictions caused by aptamer-BPA complex formation at the surface of modified electrode, the interfacial electron transfer of [Fe(CN)6](3-/4-) as a probe was blocked. Sensitive quantitative detection of BPA was carried out by monitoring the decrease of differential pulse voltammetric responses of [Fe(CN)6](3-/4-) peak current with increasing BPA concentrations. The newly developed aptasensor embraced a number of attractive features such as ease of fabrication, low detection limit, excellent selectivity, good stability and a wide linear range with respect to BPA.
Department of Chemistry, Khorramabad Branch, Islamic Azad University, Khorramabad, Iran.
Zeinab
Z
Derikvandi
Department of Chemistry, Khorramabad Branch, Islamic Azad University, Khorramabad, Iran.
Amir Reza
AR
Abbasi
Department of Chemistry, Ilam University, Ilam, Iran.
Mahmoud
M
Roushani
Department of Chemistry, Khorramabad Branch, Islamic Azad University, Khorramabad, Iran.
Zohreh
Z
Derikvand
Department of Chemistry, Khorramabad Branch, Islamic Azad University, Khorramabad, Iran. Electronic address: azadbakht.a@gmail.com.
Azadeh
A
Azadbakht
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001559:Benzhydryl Compounds / Q000032:analysis*; D055664:Electrochemical Techniques / Q000379:methods; D053768:Metal Nanoparticles / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010636:Phenols / Q000032:analysis*; D010984:Platinum / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*; D000098826:Bisphenol A Compounds
D052157:Aptamers, Nucleotide; D001559:Benzhydryl Compounds; D037742:Nanotubes, Carbon; D010636:Phenols; D010984:Platinum; D011094:Polyethyleneimine; C006780:bisphenol A; D000098826:Bisphenol A Compounds
Aptasensor; Bisphenol A; Platinum nanoparticles; Polyethyleneimine