[Effects of 1,3-diphenyl-1,3-propanedione on neurotransmitter contents of brain in mice administered with cocaine].
OBJECTIVE
To investigate the possible reversal effects of 1,3-diphenyl-1,3-propanedione (DPPD) for cocaine-induced content changes of neurotransmitters of brain in mice.


METHODS
In this study, 36 healthy ICR male mice were randomly divided into control group, cocaine group, three DPPD pretreatment groups (200, 400, and 800 mg/kg) and DPPD alone group (800 mg/kg). The mice in control group were administered intragastrically with 1% Tween 80 for 3 d, and the mice in cocaine group were administered intragastrically with 1% Tween 80 for 2 d before cocaine was injected subcutaneously on the 3rd day. The mice in the three DPPD pretreatment groups were administered intragastrically (DPPD 200, 400, and 800 mg/kg) for 3 d before cocaine was injected subcutaneously 30 min after the administration on the 3rd day. The mice in DPPD alone group were administered intragastrically with DPPD at dose of 800 mg/kg for 3 d. The mice were sacrificed 20 minutes after cocaine injection. The contents of dopamine (DA) and 5-hydroxytryptamin (5-HT) in the mice brain were determined by high performance liquid chromatography (HPLC)-fluorescence detector, the contents of glutamic acid (Glu) and γ-aminobutyric acid (GABA) in the mice brain were determined by HPLC-ultraviolet detector, and the neurotransmitter levels were compared between the groups.


RESULTS
The results showed that as compared with the control group, DA and GABA contents in cocaine group increased significantly (P<0.01 and P<0.05), while Glu content decreased (P<0.05). As compared with cocaine group, the DA levels in the three DPPD pretreatment groups (200, 400, and 800 mg/kg) all decreased significantly (P<0.01). In DPPD 200 mg/kg pre-administration group, GABA content decreased (P<0.05), and the contents of the four kinds of neurotransmitters had no statistical differences with those of the control group.


CONCLUSIONS
DPPD may have potential reversal effects of the content changes of neurotransmitters in mice brain induced by cocaine at a lower dose.
Department of Occupational and Environmental Health Sciences, Peking University School of Public Health, Beijing 100191, China.
J
J
Chen
Department of Occupational and Environmental Health Sciences, Peking University School of Public Health, Beijing 100191, China.
Y C
YC
Wang
Department of Occupational and Environmental Health Sciences, Peking University School of Public Health, Beijing 100191, China.
R
R
Cui
Department of Toxicology, Peking University School of Public Health, Beijing 100191, China.
X X
XX
Liu
Department of Toxicology, Peking University School of Public Health, Beijing 100191, China.
B X
BX
Zhang
D000818:Animals; D001921:Brain / Q000187:drug effects*; D001923:Brain Chemistry; D003042:Cocaine / Q000494:pharmacology*; D004298:Dopamine / Q000737:chemistry*; D018698:Glutamic Acid / Q000737:chemistry; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D018377:Neurotransmitter Agents / Q000737:chemistry; D010655:Phenylenediamines / Q000494:pharmacology*; D012701:Serotonin / Q000737:chemistry*; D005680:gamma-Aminobutyric Acid / Q000737:chemistry
D018377:Neurotransmitter Agents; D010655:Phenylenediamines; D012701:Serotonin; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; C004845:N,N'-diphenyl-4-phenylenediamine; D003042:Cocaine; D004298:Dopamine