Convergence chromatographic determination of camphor in the essential oil of Tanacetum parthenium L.
Feverfew (Tanacetum parthenium L., Asteraceae) is a perennial medicinal plant which has been used to alleviate the symptoms of migraine, headache and rheumatoid arthritis and possesses numerous pharmacological activities. An ultra-high-performance supercritical fluid chromatographic method (UHPSFC) was developed and validated in accordance with the International Conference on Harmonization guidelines in order to determine the camphor content of the volatile oil, which was accurate, precise, robust and selective. The method was validated for specificity, accuracy (100.2%), repeatability and intermediate precision, linearity (r2> 0.999), limit of detection (2.055 μg/mL), limit of quantification (6.228 μg/mL) and robustness. The common range of accuracy and linearity was between 0.125 and 1.000 mg/mL. Steam distillation was carried out in order to study the essential oil yield of three different T. parthenium L. samples originating from Hungarian medicinal herb collections. The camphor content of the essential oils from the aerial parts of feverfew samples from different origin was compared. Although the composition of the essential oil is well reported, a validated quantitative UHPSFC method for the determination of the constituents is presented herein for the first time.
Department of Pharmacognosy, Semmelweis University, H-1085 Budapest, Üllői str. 26., Hungary.
Krisztina
K
Végh
Richter Gedeon Plc., Compound Profiling Laboratory, H-1475 Budapest, Gyömrői str. 19-21., Hungary.
Eszter
E
Riethmüller
Department of Pharmacognosy, Semmelweis University, H-1085 Budapest, Üllői str. 26., Hungary.
Anita
A
Tóth
Department of Pharmacognosy, Semmelweis University, H-1085 Budapest, Üllői str. 26., Hungary.
Ágnes
Á
Alberti
Department of Pharmacognosy, Semmelweis University, H-1085 Budapest, Üllői str. 26., Hungary.
Szabolcs
S
Béni
Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, H-1111 Budapest, Szt. Gellért sq. 4., Hungary.
József
J
Balla
Department of Pharmacognosy, Semmelweis University, H-1085 Budapest, Üllői str. 26., Hungary.
Ágnes
Á
Kéry
D002164:Camphor / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D057230:Limit of Detection; D009822:Oils, Volatile / Q000737:chemistry*; D015203:Reproducibility of Results; D020903:Tanacetum parthenium / Q000737:chemistry*
D009822:Oils, Volatile; D002164:Camphor
Tanacetum parthenium L; camphor; convergence chromatography; method validation