Biotransformation of ginsenoside Rb1 to ginsenoside C-K by endophytic fungus Arthrinium sp. GE 17-18 isolated from Panax ginseng.
This research aimed to isolate β-glycosidase-producing endophytic fungus in Panax ginseng to achieve biotransformation of ginsenoside Rb1 to ginsenoside C-K. Of these 15 β-glucosidase-producing endophytic fungus isolated from ginseng roots, a β-glucosidase-producing endophytic fungi GE 17-18 could hydrolyse major ginsenosides Rb1 to minor ginsenoside C-K with metabolic pathways: ginsenoside Rb1→ginsenoside Rd→ginsenoside F2→ginsenoside C-K. Phylogenetic analysis of ITS gene sequences indicated that the strain GE 17-18 belongs to the genus Arthrinium and is most closely related to Arthrinium sp. HQ832803.1.


CONCLUSIONS
This is the first study to provide information of cultivable β-glycosidase-producing Endophytic fungus in Panax ginseng. The strain GE 17-18 has potential to be applied on the preparation for minor ginsenoside C-K in pharmaceutical industry.
College of Chemistry and Life Science, Anshan Normal University, Anshan, China.
Y
Y
Fu
Key Laboratory of Natural resources of Changbai Mountain and Functional Molecules, Ministry of Education, Yanbian University, Yanji, China.
Z-H
ZH
Yin
National Ginseng Products Quality Supervision Inspection Center, Yanji, China.
L-P
LP
Wu
Key Laboratory of Natural resources of Changbai Mountain and Functional Molecules, Ministry of Education, Yanbian University, Yanji, China.
C-R
CR
Yin
D001711:Biotransformation / Q000502:physiology*; D036145:Ginsenosides / Q000378:metabolism*; D006868:Hydrolysis; D005894:Panax / Q000382:microbiology*; D010802:Phylogeny; D013098:Xylariales / Q000302:isolation & purification / Q000378:metabolism*; D001617:beta-Glucosidase / Q000378:metabolism*
D036145:Ginsenosides; C407883:ginsenoside F2; C442759:ginsenoside Rb1; D001617:beta-Glucosidase; C049863:ginsenoside Rd
Panax ginseng; biotransformation; endophytic fungus; ginsenoside C-K; ginsenoside Rb1