Nuclear magnetic resonance-based serum metabolic profiling of dairy cows with footrot.
Footrot is a debilitating and contagious disease in dairy cows, caused by the Gram-negative anaerobe Dichelobacter nodosus.1H-NMR (nuclear magnetic resonance)-based metabolomics has been previously used to understand the pathology and etiology of several diseases. The objective of this study was to characterize serum from dairy cows with footrot (n=10) using1H-NMR-based metabolomics and chemometric analyses.1H-NMR spectroscopy with multivariate pattern recognition (principal component analysis and orthogonal partial least-squares discriminant analysis) was performed to identify biomarkers in cows with footrot (F) and healthy controls (C).1H-NMR analysis facilitated the identification of 21 metabolites. Among these metabolites, 4 metabolites were higher and 17 metabolites were lower in the F group than in the C group. The serum levels of 5 metabolites were significantly different (P<0.05) between the two groups. The results revealed that cows with footrot have altered carbohydrate, amino acid, lipid and energy metabolic pathways. Metabolomic approaches are a clinically useful diagnostic tool for understanding the biochemical alterations and mechanisms of several diseases.
College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Harbin 150030, P.R. China.
Jiasan
J
Zheng
Lingwei
L
Sun
Shi
S
Shu
Kuiling
K
Zhu
Chuang
C
Xu
Junsong
J
Wang
Hongbin
H
Wang
D000818:Animals; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood* / Q000378:metabolism; D020376:Dichelobacter nodosus; D005260:Female; D005535:Foot Rot / Q000097:blood* / Q000378:metabolism; D016905:Gram-Negative Bacterial Infections / Q000097:blood / Q000378:metabolism / Q000662:veterinary*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D055432:Metabolomics / Q000379:methods
D015415:Biomarkers