N-Heterocyclic Carbene-Gold(I) Complexes Conjugated to a Leukemia-Specific DNA Aptamer for Targeted Drug Delivery.
This report describes the synthesis and characterization of novel N-heterocyclic carbene (NHC)-gold(I) complexes and their bioconjugation to the CCRF-CEM-leukemia-specific aptamer sgc8c. Successful bioconjugation was confirmed by the use of fluorescent tags on both the NHC-Au(I) complex and the aptamer. Cell-viability assays indicated that the NHC-Au(I) -aptamer conjugate was more cytotoxic than the NHC-gold complex alone. A combination of flow cytometry, confocal microscopy, and cell-viability assays provided clear evidence that the NHC-Au(I) -aptamer conjugate was selective for targeted CCRF-CEM leukemia cells.
University of Florida, Department of Chemistry, P.O. Box 117200, Gainesville, FL, USA.
Weijia
W
Niu
University of Florida, Department of Chemistry, P.O. Box 117200, Gainesville, FL, USA.
Xigao
X
Chen
University of Florida, Department of Chemistry, P.O. Box 117200, Gainesville, FL, USA.
Weihong
W
Tan
University of Florida, Department of Chemistry, P.O. Box 117200, Gainesville, FL, USA. veige@chem.ufl.edu.
Adam S
0000-0002-7020-9251
AS
Veige
D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000737:chemistry; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D056831:Coordination Complexes / Q000737:chemistry*; D016503:Drug Delivery Systems; D005434:Flow Cytometry; D006046:Gold / Q000737:chemistry; D006571:Heterocyclic Compounds / Q000737:chemistry*; D006801:Humans; D007938:Leukemia / Q000188:drug therapy* / Q000473:pathology; D008697:Methane / Q000031:analogs & derivatives* / Q000737:chemistry; D018613:Microscopy, Confocal; D015394:Molecular Structure
D000970:Antineoplastic Agents; D052157:Aptamers, Nucleotide; D056831:Coordination Complexes; D006571:Heterocyclic Compounds; C030011:carbene; D006046:Gold; D008697:Methane
DNA aptamers; N-heterocyclic carbenes; bioconjugation; cytotoxicity; gold