Effect of salinity on the fatty acid and triacylglycerol composition of five haptophyte algae from the genera Coccolithophora, Isochrysis and Prymnesium determined by LC-MS/APCI.
Non-aqueous reversed-phase high-performance liquid chromatography (NARP-HPLC) with atmospheric pressure chemical ionization (APCI) was used for separation of triacylglycerols from five strains of haptophyte algae (genera Coccolithophora, Isochrysis, and Prymnesium). This study describes the separation and identification of C18 polyunsaturated triacylglycerols containing stearidonic and octadecapentaenoic fatty acids, including their regioisomers. Salinity affects the proportion of saturated and unsaturated fatty acids. The biosynthesis of C18 polyunsaturated triacylglycerols was found to be very stereospecific and to depend on the salinity of cultivation media, asymmetric regioisomers predominating at low salinity (sn-OpOpSt and/or PoStSt) and symmetric ones at high salinity (sn-OpStOp and or StPoSt).
Charles University in Prague, Faculty of Science, Department of Ecology, Viničná 7, 128 44 Prague, Czech Republic.
Linda
L
Nedbalová
Charles University in Prague, Faculty of Science, Department of Ecology, Viničná 7, 128 44 Prague, Czech Republic.
Antonín
A
Střížek
Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20 Prague, Czech Republic.
Karel
K
Sigler
Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20 Prague, Czech Republic. Electronic address: rezanka@biomed.cas.cz.
Tomáš
T
Řezanka
D002851:Chromatography, High Pressure Liquid; D005227:Fatty Acids / Q000032:analysis*; D015525:Fatty Acids, Omega-3 / Q000737:chemistry; D005231:Fatty Acids, Unsaturated / Q000737:chemistry; D005858:Germany; D058087:Haptophyta / Q000737:chemistry* / Q000235:genetics; D015394:Molecular Structure; D054712:Salinity; D013237:Stereoisomerism; D014280:Triglycerides / Q000032:analysis*
D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D005231:Fatty Acids, Unsaturated; D014280:Triglycerides; C062895:stearidonic acid
Coccolithophyceae; Haptophyte algae (genera Coccolithophora, Isochrysis and Prymnesium); Liquid chromatography-mass spectrometry; Octadecapentaenoic acid; Salinity; Stearidonic acid