Downregulation of microRNA-206 promotes invasion and angiogenesis of triple negative breast cancer.
Triple negative breast tumors don't respond to Tamoxifen and Herceptin, two of the most effective medications for treating breast cancer. Additionally, triple negative breast cancer (TNBC) intrinsically resists or will eventually acquire resistance to chemotherapy. The purpose of this study is to understand better the molecular basis of TNBC as well as develop new therapeutic strategies against it. Here, we analyzed miRNA-206 expression levels in breast cancer cell lines and tissues. In addition, we investigated whether miR-206 mimics inhibited TNBC tumor invasion and angiogenesis. The results showed that miR-206 was downregulated in TNBC compared to non-TNBC cell lines and tissues. Additionally, the decreased levels of miR-206 were inversely consistent with expression levels of VEGF. Furthermore, the forced expression of miR-206 in the mimic-transfected TNBC cells downregulated VEGF, MAPK3, and SOX9 expression levels. The miR-206 mimics inhibited TNBC breast cell invasion and angiogenesis. These findings demonstrate for the first time the involvement of miRNA-206 in TNBC invasion and angiogenesis and suggest that miR-206 may be an efficient agent for therapy of TNBC.
Department of Radiology, Emory University, Atlanta, GA 30322, USA; Winship Cancer Institute, Emory University, Atlanta, GA 30322, USA. Electronic address: zliang@emory.edu.
Zhongxing
Z
Liang
Department of Radiology, Emory University, Atlanta, GA 30322, USA; Department of Thyroid Surgery, China-Japan Union Hospital of Jilin University, Changchun, China.
Xuehai
X
Bian
Department of Radiology, Emory University, Atlanta, GA 30322, USA; Winship Cancer Institute, Emory University, Atlanta, GA 30322, USA.
Hyunsuk
H
Shim
D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D048052:Mitogen-Activated Protein Kinase 3 / Q000235:genetics; D009361:Neoplasm Invasiveness; D009389:Neovascularization, Pathologic; D055758:SOX9 Transcription Factor / Q000235:genetics; D064726:Triple Negative Breast Neoplasms / Q000235:genetics / Q000473:pathology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
C546087:MIRN206 microRNA, human; D035683:MicroRNAs; D055758:SOX9 Transcription Factor; C527932:SOX9 protein, human; D042461:Vascular Endothelial Growth Factor A; D048052:Mitogen-Activated Protein Kinase 3
Angiogenesis; Invasion; MicroRNA; Triple negative breast cancer; VEGF