Berberis aristata Ameliorates Adjuvant-Induced Arthritis by Inhibition of NF-κB and Activating Nuclear Factor-E2-related Factor 2/hem Oxygenase (HO)-1 Signaling Pathway.
The present study was carried out to investigate the anti-arthritic activity of Berberis aristata hydroalcoholic extract (BAHE) in formaldehyde-induced arthritis and adjuvant-induced arthritis (AIA) model. Arthritis was induced by administration of either formaldehyde (2% v/v) or CFA into the subplantar surface of the hind paw of the animal. In formaldehyde-induced arthritis and AIA, treatment of BAHE at doses 50, 100 and 200 mg/kg orally significantly decreased joint inflammation as evidenced by decrease in joint diameter and reduced inflammatory cell infiltration in histopathological examination. BAHE treatment demonstrated dose-dependent improvement in the redox status of synovium (decrease in GSH, MDA, and NO levels and increase in SOD and CAT activities). The beneficial effect of BAHE was substantiated with decreased expression of inflammatory markers such as IL-1β, IL-6, TNF-R1, and VEGF by immunohistochemistry analysis in AIA model. BAHE increased HO-1/Nrf-2 and suppressed NF-κB mRNA and protein expression in adjuvant immunized joint. Additionally, BAHE abrogated degrading enzymes, as there was decreased protein expression of MMP-3 and -9 in AIA. In conclusion, we demonstrated the anti-arthritic activity of Berberis aristata hydroalcoholic extract via the mechanism of inhibition of NF-κB and activation of Nrf-2/HO-1.
a Department of Pharmacology , All India Institute of Medical Sciences (AIIMS) , New Delhi , India.
Rohit
R
Kumar
a Department of Pharmacology , All India Institute of Medical Sciences (AIIMS) , New Delhi , India.|b IPRD-05 (Chemical Safety and Regulatory Affairs) , Evalueserve SEZ (Gurgaon) Pvt. Ltd ., Gurgaon , India.
Vinod
V
Nair
a Department of Pharmacology , All India Institute of Medical Sciences (AIIMS) , New Delhi , India.
Yogendra Kumar
YK
Gupta
a Department of Pharmacology , All India Institute of Medical Sciences (AIIMS) , New Delhi , India.
Surender
S
Singh
c Research Officer, Natural Remedies Pvt. Ltd ., Bangalore , India.
S
S
Arunraja
D000284:Administration, Oral; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001169:Arthritis, Experimental / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D028142:Berberis / Q000737:chemistry*; D002374:Catalase / Q000235:genetics / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D005557:Formaldehyde; D005620:Freund's Adjuvant; D005786:Gene Expression Regulation; D005978:Glutathione / Q000819:agonists / Q000276:immunology; D006170:Gum Arabic; D006419:Heme Oxygenase (Decyclizing) / Q000235:genetics / Q000276:immunology*; D008297:Male; D008315:Malondialdehyde / Q000037:antagonists & inhibitors / Q000276:immunology; D051267:NF-E2-Related Factor 2 / Q000235:genetics / Q000276:immunology*; D016328:NF-kappa B / Q000235:genetics / Q000276:immunology*; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000276:immunology; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction; D013482:Superoxide Dismutase / Q000235:genetics / Q000276:immunology; D013583:Synovial Membrane / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D013643:Tarsus, Animal / Q000187:drug effects / Q000276:immunology / Q000473:pathology
D000894:Anti-Inflammatory Agents, Non-Steroidal; D051267:NF-E2-Related Factor 2; D016328:NF-kappa B; C495637:Nfe2l2 protein, rat; D010936:Plant Extracts; D005557:Formaldehyde; D009569:Nitric Oxide; D008315:Malondialdehyde; D006170:Gum Arabic; D005620:Freund's Adjuvant; D002374:Catalase; D006419:Heme Oxygenase (Decyclizing); C491523:Hmox1 protein, rat; D013482:Superoxide Dismutase; D005978:Glutathione
Adjuvant-induced arthritis; Berberis aristata; angiogenesis; pro-inflammatory cytokines