Immunohistochemical Expression of VEGF and Podoplanin in Uterine Cervical Squamous Intraepithelial Lesions.
VEGF and podoplanin (PDPN) have been identified as angiogenesis and/or lymphangiogenesis regulators and might be essential to restrict tumor growth, progression, and metastasis. In the present study, we evaluate the association between the expression of these markers and CIN grade. Immunohistochemistry was performed in 234 uterine cervical samples using conventional histologic sections or TMA with the monoclonal antibodies to VEGF (C-1 clone) and podoplanin (D2-40 clone). Positive-staining rates of VEGF in 191 CIN specimens were significantly associated with histological grade (P < 0.001). Negative and/or focal immunostaining for PDPN were more frequent in CIN 3 (P = 0.016). We found that patients with CIN 3 more frequently had strong and more diffuse staining for VEGF and diminished staining for PDPN (P = 0.018). Strong and more diffuse VEGF immunoexpressions in CIN 2 and CIN 3 were detected when compared to CIN 1. Negative and/or focal PDPN immunoexpression appear to be more frequent in CIN 3. Moderate to strong VEGF expression may be a tendency among patients with high-grade lesions and diminished PDPN expression.
Gynecological Disease Prevention Nucleus (NUPREV), Department of Gynecology, Federal University of São Paulo, UNIFESP/EPM, 380 Borges Lagoa Street, 04038-000 São Paulo, SP, Brazil.
Patrícia Napoli
PN
Belfort-Mattos
Department of Pathology, Federal University of São Paulo, UNIFESP/EPM, 740 Botucatu Street, 04023-062 São Paulo, SP, Brazil.
Gustavo Rubino de Azevedo
GR
Focchi
Gynecological Disease Prevention Nucleus (NUPREV), Department of Gynecology, Federal University of São Paulo, UNIFESP/EPM, 380 Borges Lagoa Street, 04038-000 São Paulo, SP, Brazil.
Julisa Chamorro Lascasas
JC
Ribalta
Gynecological Disease Prevention Nucleus (NUPREV), Department of Gynecology, Federal University of São Paulo, UNIFESP/EPM, 380 Borges Lagoa Street, 04038-000 São Paulo, SP, Brazil.
Tatiana
T
Megale De Lima
Gynecological Disease Prevention Nucleus (NUPREV), Department of Gynecology, Federal University of São Paulo, UNIFESP/EPM, 380 Borges Lagoa Street, 04038-000 São Paulo, SP, Brazil.
Carmen Regina
CR
Nogueira Carvalho
Gynecological Disease Prevention Nucleus (NUPREV), Department of Gynecology, Federal University of São Paulo, UNIFESP/EPM, 380 Borges Lagoa Street, 04038-000 São Paulo, SP, Brazil.
Fernanda
F
Kesselring Tso
Gynecological Disease Prevention Nucleus (NUPREV), Department of Gynecology, Federal University of São Paulo, UNIFESP/EPM, 380 Borges Lagoa Street, 04038-000 São Paulo, SP, Brazil.
Neila Maria
NM
De Góis Speck
D000328:Adult; D014408:Biomarkers, Tumor / Q000378:metabolism*; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007124:Immunoenzyme Techniques; D008562:Membrane Glycoproteins / Q000378:metabolism*; D009367:Neoplasm Staging; D011379:Prognosis; D012189:Retrospective Studies; D065310:Squamous Intraepithelial Lesions of the Cervix / Q000378:metabolism / Q000473:pathology*; D002583:Uterine Cervical Neoplasms / Q000378:metabolism / Q000473:pathology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*; D002578:Uterine Cervical Dysplasia / Q000378:metabolism / Q000473:pathology*; D000098853:Podoplanin
D014408:Biomarkers, Tumor; D008562:Membrane Glycoproteins; D042461:Vascular Endothelial Growth Factor A; C117468:PDPN protein, human; D000098853:Podoplanin; C467484:VEGFA protein, human