Immunostimulation by cytosine-phosphate-guanine oligodeoxynucleotides in combination with IL-2 can improve the success rate of karyotype analysis in chronic lymphocytic leukaemia.
OBJECTIVE
To assess whether immunostimulatory cytosine-phosphate-guanine oligodeoxynucleotides (CpG-ODN) combined with interleukin-2 (IL-2) improves the number of mitotic metaphases and the detection rate of chromosomal abnormalities in chronic lymphocytic leukaemia (CLL).


METHODS
Bone marrow specimens were collected from 36 patients with CLL. CLL cells were cultured with CpG-ODN type DSP30 plus IL-2 for 72 h, following which R-banding analysis was conducted. Conventional culture without the immunostimulant served as the control group. The incidence of genetic abnormalities was measured by fluorescence in situ hybridisation (FISH) using a panel of five specific probes: D13S25 (13q14.3), RB1 (13q14), P53 (17p13), ATM (11q22.3) and CSP12 (trisomy 12, +12).


RESULTS
In the control group, chromosome analysis achieved a success rate of only 22.2, and 11.1% of abnormal karyotypes were detected. After immunostimulation with DSP30 plus IL-2, chromosome analysis achieved a success rate of up to 91.6, and 41.6% of abnormal karyotypes were detected. FISH analysis detected 77.7% of abnormalities. FISH combined with CpG-ODN DSP30 plus IL-2 improved the detection rate of chromosomal abnormalities in CLL to 83.3%.


CONCLUSIONS
CpG-ODN DSP30 combined with IL-2 is effective in improving the detection rate of chromosomal abnormalities in CLL cells. This combination with FISH analysis is conducive to increasing the detection rate of genetic abnormalities in CLL.
a Fujian Provincial Key Laboratory of Hematology, Fujian Institute of Hematology , Fujian Medical University Union Hospital , Fujian , China.
Xiaolan
X
Lin
a Fujian Provincial Key Laboratory of Hematology, Fujian Institute of Hematology , Fujian Medical University Union Hospital , Fujian , China.
Jiadi
J
Chen
a Fujian Provincial Key Laboratory of Hematology, Fujian Institute of Hematology , Fujian Medical University Union Hospital , Fujian , China.
Huifang
H
Huang
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007376:Interleukin-2 / Q000276:immunology*; D007621:Karyotyping / Q000379:methods*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009841:Oligonucleotides
C539572:CpG-ODN DSP30; C508594:IL2 protein, human; D007376:Interleukin-2; D009841:Oligonucleotides
Chronic lymphocytic leukaemia; CpG oligodeoxynucleotide; cytogenetics; immunostimulation; interleukin-2; karyotype