A re-evaluation of anti-NA-14 antibodies in patients with primary Sjögren's syndrome: Significant role of interferon-γ in the production of autoantibodies against NA-14.
Novel autoantibodies against nuclear antigen of 14 kDa (NA-14)/Sjögren's syndrome nuclear antigen-1 (SSNA-1) are predominantly recognized in sera of patients with primary Sjögren's syndrome (pSS). However, the detailed characteristics of the anti-NA-14 antibody remain unknown. Here, we sought to clarify the characteristics of anti-SSNA-1/NA-14 antibodies and the mechanisms of autoantibody production using sera from patients with connective tissue diseases (including pSS), autoimmune sera reacting with standard autoantigens (SS-A/Ro and/or SS-B/La, ds DNA, Scl-70 and Jo-1), and normal healthy controls (NHCs). Anti-NA-14 antibodies were predominantly recognized in sera from patients with pSS and in autoimmune sera reacting with thSS-A/Ro and/or -SS-B/Lo. Indirect immunofluorescence analysis showed that NA-14 was strongly expressed in mitotic-phase cells. Patients with pSS having anti-NA-14 antibodies exhibited significant elevation of serum IP-10 and BAFF compared to that in patients with pSS without anti-NA-14 antibodies and NHCs. Thus, our data demonstrated that anti-NA-14 antibodies could be classified as novel autoantibodies reacting with mitosis-related autoantigens predominantly recognized in pSS. Moreover, interferon-γ played an important role in the production of anti-NA-14 autoantibodies as patients with pSS having anti-NA-14 antibodies exhibited increased serum levels of IP-10 and BAFF.
a Department of Internal Medicine and Rheumatology , Faculty of Medicine, Juntendo University , Tokyo , Japan .
Kaori
K
Uomori
a Department of Internal Medicine and Rheumatology , Faculty of Medicine, Juntendo University , Tokyo , Japan .
Kazuhisa
K
Nozawa
b Juntendo University Urayasu Hospital, Juntendo University Graduate School of Medicine , Urayasu , Japan , and.
Keigo
K
Ikeda
a Department of Internal Medicine and Rheumatology , Faculty of Medicine, Juntendo University , Tokyo , Japan .
Kentaro
K
Doe
a Department of Internal Medicine and Rheumatology , Faculty of Medicine, Juntendo University , Tokyo , Japan .
Yusuke
Y
Yamada
a Department of Internal Medicine and Rheumatology , Faculty of Medicine, Juntendo University , Tokyo , Japan .
Ayako
A
Yamaguchi
b Juntendo University Urayasu Hospital, Juntendo University Graduate School of Medicine , Urayasu , Japan , and.
Maki
M
Fujishiro
b Juntendo University Urayasu Hospital, Juntendo University Graduate School of Medicine , Urayasu , Japan , and.
Mikiko
M
Kawasaki
b Juntendo University Urayasu Hospital, Juntendo University Graduate School of Medicine , Urayasu , Japan , and.
Shinji
S
Morimoto
b Juntendo University Urayasu Hospital, Juntendo University Graduate School of Medicine , Urayasu , Japan , and.
Kenji
K
Takamori
b Juntendo University Urayasu Hospital, Juntendo University Graduate School of Medicine , Urayasu , Japan , and.
Iwao
I
Sekigawa
c Department of Oral Biology , University of Florida , Gainesville , FL , USA.
Edward K L
EK
Chan
a Department of Internal Medicine and Rheumatology , Faculty of Medicine, Juntendo University , Tokyo , Japan .
Yoshinari
Y
Takasaki
D000328:Adult; D000368:Aged; D000917:Antibody Formation / Q000276:immunology*; D001323:Autoantibodies / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology*; D015415:Biomarkers; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D009687:Nuclear Proteins / Q000276:immunology*; D012859:Sjogren's Syndrome / Q000097:blood / Q000175:diagnosis / Q000276:immunology* / Q000378:metabolism*
D001323:Autoantibodies; D001324:Autoantigens; D015415:Biomarkers; C110204:NA14 nuclear autoantigen; D009687:Nuclear Proteins; D007371:Interferon-gamma
Nuclear autoantigen 14 kDa (NA-14); Sjögren’s syndrome; autoantibody; autoantigen; interferon-γ