Dopaminergic Modulation of Excitatory Transmission in the Anterior Cingulate Cortex of Adult Mice.
Dopamine (DA) possesses potent neuromodulatory properties in the central nervous system. In the anterior cingulate cortex, α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors (AMPAR) are key ion channels in mediating nerve injury induced long-term potentiation (LTP) and chronic pain phenotype. In the present study, we reported the effects of DA on glutamate mediated excitatory post-synaptic currents (EPSCs) in pyramidal neurons of layer II/III of the ACC in adult mice. Bath application of DA (50 μM) caused a significant, rapid and reversible inhibition of evoked EPSCs (eEPSC). This inhibitory effect is dose-related and was absent in lower concentration of DA (5 μM). Furthermore, selective postsynaptic application of GDP-β-S (1.6 mM) in the internal solution completely abolished the inhibitory effects of DA (50 μM). We also investigated modulation of spontaneous EPSCs (sEPSCs) and TTX sensitive, miniature EPSCs (mEPSCs) by DA. Our results indicated mixed effects of potentiation and inhibition of frequency and amplitude for sEPSCs and mEPSCs. Furthermore, high doses of SCH23390 (100 μM) and sulpiride (100 μM) revealed that, inhibition of eEPSCs is mediated by postsynaptic D2-receptors (D2R). Our finding posits a pre- and postsynaptic mode of pyramidal neuron EPSC modulation in mice ACC by DA.
Department of Physiology, Faculty of Medicine, University of Toronto Centre for the Study of Pain, Toronto, ON, Canada.
Soroush
S
Darvish-Ghane
Department of Physiology, Faculty of Medicine, University of Toronto Centre for the Study of Pain, Toronto, ON, Canada.
Manabu
M
Yamanaka
Department of Physiology, Faculty of Medicine, University of Toronto Centre for the Study of Pain, Toronto, ON, Canada Center for Neuron and Disease, Frontier Institutes of Science and Technology, Xi'an Jiaotong University, Xi'an, Shanxi, China min.zhuo@utoronto.ca.
Min
M
Zhuo
D000375:Aging / Q000502:physiology; D000818:Animals; D001552:Benzazepines / Q000494:pharmacology; D004298:Dopamine / Q000494:pharmacology*; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects; D006179:Gyrus Cinguli / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008810:Mice, Inbred C57BL; D008954:Models, Biological; D009433:Neural Inhibition / Q000187:drug effects; D017966:Pyramidal Cells / Q000187:drug effects / Q000378:metabolism; D017448:Receptors, Dopamine D2 / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism; D018092:Kainic Acid Receptors / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013469:Sulpiride / Q000494:pharmacology; D009435:Synaptic Transmission / Q000187:drug effects*; D013779:Tetrodotoxin / Q000633:toxicity; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / Q000378:metabolism
D001552:Benzazepines; D004298:Dopamine; D017448:Receptors, Dopamine D2; D043562:Receptors, G-Protein-Coupled; D018092:Kainic Acid Receptors; D013469:Sulpiride; D013779:Tetrodotoxin; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; C534628:SCH 23390
AMPA/KA receptors; Anterior cingulate cortex; D2 receptors; dopamine; evoked excitatory postsynaptic currents; miniature excitatory postsynaptic currents; spontaneous excitatory postsynaptic currents