Cell Culture Techniques: Corticosteroid Treatment in A549 Human Lung Epithelial Cell.
Experimentation with cell cultures is widespread in different areas of basic science as well as in the development of biotechnology applied to medicine. Cellular models are applied to the study of respiratory diseases. In this chapter, we present a protocol of basic cell culture using A549 Human lung adenocarcinoma epithelial cells as model. Corticosteroid therapy is used to treat respiratory diseases such asthma. Thus, we also describe a protocol of lung epithelial cell culture treated with dexamethasone to illustrate an example of monitoring the effects of a drug in a lung cell culture assay.
Department of Clinical Biochemistry, University Hospital of Salamanca, Paseo de San Vicente 58, Salamanca, 37007, Spain. elemarcos@hotmail.com.|Salamanca Institute for Biomedical Research (IBSAL), Salamanca, Spain. elemarcos@hotmail.com.
Elena
E
Marcos-Vadillo
Salamanca Institute for Biomedical Research (IBSAL), University Hospital of Salamanca, Salamanca, Spain.|Department of Biomedical and Diagnostic Sciences, University of Salamanca, Salamanca, Spain.
Asunción
A
García-Sánchez
D000072283:A549 Cells; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival; D003907:Dexamethasone / Q000494:pharmacology*; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects; D005938:Glucocorticoids / Q000494:pharmacology*; D006801:Humans; D008168:Lung / Q000166:cytology* / Q000187:drug effects
D005938:Glucocorticoids; D003907:Dexamethasone
Cell culture; Cell freezing; Cell thawing; Corticosteroids; Mycoplasma; Subculture