Dapsone improves functional deficit and diminishes brain damage evaluated by 3-Tesla magnetic resonance image after transient cerebral ischemia and reperfusion in rats.
Stroke is a frequent cause of death and the first of disability in the world population. We have shown that dapsone acts as an antioxidant, antiinflammatory and antiapoptotic agent after brain Ischemia reperfusion (I/R) in rats; however, its therapeutic efficacy, measured by imaging has not been characterized. In this context, the aim of this study was to evaluate the neuroprotective effect of dapsone by magnetic resonance imaging (MRI) and to correlate imaging markers with motor function and oxidative stress after transient cerebral ischemia and reperfusion (I/R). We used male rats throughout the experiment. Functional deficit after I/R was assessed by using Longa scale. The area of brain tissue damage was measured by histology. The nuclear factor erythroid 2-related factor 2 (Nrf-2) and the amount of reactive oxygen species (ROS) were measured as biomarkers of oxidative stress. Finally, difussion tensor MRI was employed to measure the fractional anisotropy (FA), as a MRI marker of the pathophysiologic brain status. Results showed a better functional recovery and less damaged tissue in animals treated with dapsone vs control group. The values of FA were higher in animals receiving treatment, indicating a better preservation of brain structure. At early stages of the damage, dapsone was able to reduce both oxidative markers (Nrf-2 and ROS). Our findings provide new evidence for the efficacy of dapsone when administered during the acute phase after I/R and that quantitative sequences of MRI are useful for characterizing its potential therapeutic benefits after stroke.
Departamento de Neuroquímica, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez S.S.A., Mexico.
Araceli
A
Diaz-Ruiz
Escuela de Medicina, Universidad Panamericana, México D.F., Mexico.
Ernesto
E
Roldan-Valadez
Laboratorio de Neuropatología Experimental, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez S.S.A., Mexico.
Alma
A
Ortiz-Plata
Consejo Nacional de Ciencia y Tecnología, Unidad de Investigación Médica en Centro Médico Nacional Siglo XXI Enfermedades Neurológicas, Hospital de Especialidades, Instituto Mexicano del Seguro Social, Mexico.
Rodrigo
R
Mondragón-Lozano
Facultad de Medicina Veterinaria de la Universidad Nacional Autónoma de México, Mexico.
Yessica
Y
Heras-Romero
Laboratorio de Neuropatología Experimental, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez S.S.A., Mexico.
Marisela
M
Mendez-Armenta
Departamento de Neuroquímica, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez S.S.A., Mexico.
Laura
L
Osorio-Rico
Laboratorio de Neuropatología Experimental, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez S.S.A., Mexico.
Concepción
C
Nava-Ruiz
Departamento de Neuroquímica, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez S.S.A., Mexico; Departamento de Sistemas Biológicos de la Universidad Autónoma Metropolitana, Unidad Xochimilco, Mexico. Electronic address: crios@correo.xoc.uam.mx.
Camilo
C
Ríos
D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D002545:Brain Ischemia / Q000150:complications / Q000000981:diagnostic imaging* / Q000188:drug therapy* / Q000473:pathology; D003622:Dapsone / Q000008:administration & dosage*; D008279:Magnetic Resonance Imaging; D008297:Male; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D018696:Neuroprotective Agents / Q000008:administration & dosage*; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D020127:Recovery of Function / Q000187:drug effects; D015427:Reperfusion Injury / Q000150:complications / Q000000981:diagnostic imaging* / Q000188:drug therapy* / Q000473:pathology; D020521:Stroke / Q000150:complications*
D051267:NF-E2-Related Factor 2; D018696:Neuroprotective Agents; C495636:Nfe2l2 protein, mouse; D017382:Reactive Oxygen Species; D003622:Dapsone
Dapsone; Magnetic resonance imaging; Neuroprotection; Transient cerebral ischemia and reperfusion