Atomic force microscopy combined with optical microscopy for cells investigation.
This review reports on the combined use of the atomic force microscopy (AFM) and several type of optical/fluorescence/laser scanning microscopy for investigating cells. It is shown that the hybrid systems of AFM with optical-derived microscopies enable to study in detail cell surface properties (such as topography), their mechanical properties (e.g., Young's modulus) mechanotransduction phenomena and allow to gain insight into biological-related pathways and mechanisms in the complex nanoworld of cells. Microsc. Res. Tech. 80:109-123, 2017. © 2016 Wiley Periodicals, Inc.
Dipartimento di Matematica e Fisica "Ennio De Giorgi", Università del Salento Via Monteroni, 73100, Lecce, Italy.|Euromediterranean Center for Nanomaterial Modelling and Technology (ECMT) of Consiglio Nazionale delle Ricerche, Istituto Nanoscienze, Via Arnesano 16, Lecce, Italy.
Mariafrancesca
M
Cascione
Dipartimento di Matematica e Fisica "Ennio De Giorgi", Università del Salento Via Monteroni, 73100, Lecce, Italy.
Valeria
V
de Matteis
Dipartimento di Matematica e Fisica "Ennio De Giorgi", Università del Salento Via Monteroni, 73100, Lecce, Italy.|Euromediterranean Center for Nanomaterial Modelling and Technology (ECMT) of Consiglio Nazionale delle Ricerche, Istituto Nanoscienze, Via Arnesano 16, Lecce, Italy.
Rosaria
R
Rinaldi
CNR Nantotec-Istituto di Nanotecnologia, Polo di Nanotecnologia c/o Campus Ecoteckne, Via Monteroni, 73100, Lecce, Italy.
Stefano
http://orcid.org/0000-0001-5912-7565
S
Leporatti
D000818:Animals; D045744:Cell Line, Tumor; D002468:Cell Physiological Phenomena; D006801:Humans; D007091:Image Processing, Computer-Assisted; D040542:Mechanotransduction, Cellular; D008853:Microscopy; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D013499:Surface Properties
atomic force microscopy; cells; confocal laser scanning microscopy; fluorescence microscopy; total internal reflection fluorescence microscopy