T7 polymerase-driven transcription is downregulated in metacyclic promastigotes and amastigotes of Leishmania mexicana.
In our previous work we established a T7 polymerase-driven Tetracycline-inducible protein expression system in Leishmania mexicana (Biagi, 1953). We used this system to analyse gene expression profiles during development of L. mexicana in procyclic and metacyclic promastigotes and amastigotes. The transcription of the gene of interest and the T7 polymerase genes was significantly reduced upon cell differentiation. This regulation is not locus-specific. It depends on untranslated regions flanking open reading frames of the genes analysed. In this paper, we report that the previously established conventional inducible protein expression system may not be suitable for studies on differentiation of species of Leishmania Ross, 1903 and protein expression systems might have certain limitations.
Life Science Research Centre, Faculty of Science, University of Ostrava, Ostrava, Czech Republic;
Aygul
A
Ishemgulova
Life Science Research Centre, Faculty of Science, University of Ostrava, Ostrava, Czech Republic;
Natalya
N
Kraeva
Biology Centre, Institute of Parasitology, Czech Academy of Sciences, Ceske Budejovice, Czech Republic;
Drahomira
D
Faktorova
Life Science Research Centre, Faculty of Science, University of Ostrava, Ostrava, Czech Republic;
Lucie
L
Podesvova
Biology Centre, Institute of Parasitology, Czech Academy of Sciences, Ceske Budejovice, Czech Republic.|Faculty of Sciences, University of South Bohemia, Ceske Budejovice, Czech Republic.|Canadian Institute for Advanced Research, Toronto, Canada.
Julius
J
Lukes
Life Science Research Centre, Faculty of Science, University of Ostrava, Ostrava, Czech Republic.|Biology Centre, Institute of Parasitology, Czech Academy of Sciences, Ceske Budejovice, Czech Republic.|Department of Pathology, Albert Einstein College of Medicine, Bronx, NY, USA.|Institute of Environmental Technologies, Faculty of Science, University of Ostrava, Ostrava, Czech Republic.
Vyacheslav
V
Yurchenko
D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D005786:Gene Expression Regulation; D007894:Leishmania mexicana / Q000201:enzymology / Q000235:genetics*; D008018:Life Cycle Stages / Q000235:genetics
D004259:DNA-Directed DNA Polymerase
Leishmania mexicana; Tet-inducible system; gene expression; untranslated regions