Tetradecyl 2,3-dihydroxybenzoate promotes functional recovery after spinal cord injury in adult rats.
Tetradecyl 2,3-dihydroxybenzoate (ABG001) is a small molecule separated from gentian extract that has a similar effect to nerve growth factor. It is not clear whether it can promote functional recovery in animals suffering from a central nervous system injury. In order to explore the role of ABG001 in restoration of tissue structure and motor function of rats with spinal cord injury (SCI), ABG001 (0.4 mg/kg) was administered intraperitoneally. Subsequently, behavioral assessments and morphological studies were performed to detect recovery of hind limb motor function and neuroregeneration. The results showed that compared with DMSO group, the rats in the ABG treatment group had better performance in BBB score and grip strength test (P < 0.05), the area of necrosis was smaller (P < 0.05), GFAP expression was significantly reduced (P < 0.01), and Map-2 expression was significantly increased (P < 0.01). Additionally, after ABG treatment, the number of fluorogold positive cells transported reversely to red nucleus increased (P < 0.05). The results suggest that ABG001 can promote recovery of hind limb motor function in rats with SCI, which may be related to its functions of inhibiting glial cell proliferation and promoting neuroregeneration.
Department of Clinical Medicine, School of Medicine, Zhejiang University City College, Hangzhou, Zhejiang, China.
Y M
YM
Ding
Department of Clinical Medicine, School of Medicine, Zhejiang University City College, Hangzhou, Zhejiang, China.
J Y
JY
Zhu
Department of Clinical Medicine, School of Medicine, Zhejiang University City College, Hangzhou, Zhejiang, China.
S J
SJ
Zhang
Department of Clinical Medicine, School of Medicine, Zhejiang University City College, Hangzhou, Zhejiang, China.
S S
SS
Zhang
Department of Clinical Medicine, School of Medicine, Zhejiang University City College, Hangzhou, Zhejiang, China.
C
C
Wang
Department of Basic Medical Science, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
L L
LL
Wang
Department of Basic Medical Science, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
X
X
Zhang
D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D062385:Hydroxybenzoates / Q000494:pharmacology*; D009043:Motor Activity / Q000187:drug effects; D020932:Nerve Growth Factor / Q000378:metabolism; D009416:Nerve Regeneration / Q000502:physiology; D009457:Neuroglia / Q000378:metabolism / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000187:drug effects; D013119:Spinal Cord Injuries / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology
D005904:Glial Fibrillary Acidic Protein; D062385:Hydroxybenzoates; C571109:tetradecyl-2,3-dihydroxybenzoate; D020932:Nerve Growth Factor