Expression of Cornified Envelope Proteins in Skin and Its Relationship with Atopic Dermatitis Phenotype.
Changes in the expression of cornified envelope (CE) proteins are thought to affect the development and course of atopic dermatitis (AD). The aim of this study was to examine the expression level of CE proteins in order to identify new molecular markers of the AD phenotype. Expression levels of CE proteins were evaluated in the skin of patients with AD (38 biopsies) and healthy subjects (26 biopsies). Levels of FLG, FLG2 and SPRR3 mRNAs and proteins were reduced in AD skin. Levels of LELP-1 and SPRR1A transcripts and proteins were significantly increased in AD skin. SPRR3v2 mRNA level in non-lesional AD skin correlated with severity of AD, and SPRR3 protein level in non-lesional AD skin correlated inversely with pruritus. FLG protein level in AD skin correlated inversely with severity of AD. These results point to SPRR3 as an important factor in AD and itch.
Department of Dermatology, Venereology and Allergology, Medical University of Gdańsk, 80-211 Gdańsk, Poland. mtrzeciak@gumed.edu.pl.
Magdalena
M
Trzeciak
Monika
M
Sakowicz-Burkiewicz
Martyna
M
Wesserling
Daria
D
Dobaczewska
Jolanta
J
Gleń
Roman
R
Nowicki
Tadeusz
T
Pawelczyk
D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D001706:Biopsy; D016022:Case-Control Studies; D055233:Cornified Envelope Proline-Rich Proteins / Q000235:genetics* / Q000378:metabolism; D003876:Dermatitis, Atopic / Q000235:genetics* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D000091344:Filaggrin Proteins; D015870:Gene Expression; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007381:Intermediate Filament Proteins / Q000235:genetics; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D060888:Real-Time Polymerase Chain Reaction; D009418:S100 Proteins / Q000235:genetics; D012720:Severity of Illness Index
D015415:Biomarkers; D055233:Cornified Envelope Proline-Rich Proteins; C000717249:FLG protein, human; C000717250:FLG2 protein, human; D000091344:Filaggrin Proteins; D007381:Intermediate Filament Proteins; C520829:LELP1 protein, human; D009418:S100 Proteins; C524531:SPRR1A protein, human; C112254:SPRR3 protein, human; C524533:SPRR1B protein, human; D007073:Immunoglobulin E