Construction of an Immobilized Thermophilic Esterase on Epoxy Support for Poly(ε-caprolactone) Synthesis.
Developing an efficient immobilized enzyme is of great significance for improving the operational stability of enzymes in poly(ε-caprolactone) synthesis. In this paper, a thermophilic esterase AFEST from the archaeon Archaeoglobus fulgidus was successfully immobilized on the epoxy support Sepabeads EC-EP via covalent attachment, and the immobilized enzyme was then employed as a biocatalyst for poly(ε-caprolactone) synthesis. The enzyme loading and recovered activity of immobilized enzyme was measured to be 72 mg/g and 10.4 U/mg using p-nitrophenyl caprylate as the substrate at 80 °C, respectively. Through the optimization of reaction conditions (enzyme concentration, temperature, reaction time and medium), poly(ε-caprolactone) was obtained with 100% monomer conversion and low number-average molecular weight (Mn < 1300 g/mol). Further, the immobilized enzyme exhibited excellent reusability, with monomer conversion values exceeding 75% during 15 batch reactions. Finally, poly(ε-caprolactone) was enzymatically synthesized with an isolated yield of 75% and Mn value of 3005 g/mol in a gram-scale reaction.
Department of Colorectal Surgery, the Second Hospital of Jilin University, Changchun 130041, China. hren@jlu.edu.cn.
Hui
H
Ren
Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China. xingzhen14@mails.jlu.edu.cn.
Zhen
Z
Xing
Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China. yjb13804460320@sina.com.
Jiebing
J
Yang
Department of Polymer Science, College of Chemistry, Jilin University, Changchun 130012, China. jiangw0909215@163.com.
Wei
W
Jiang
Department of Polymer Science, College of Chemistry, Jilin University, Changchun 130012, China. gzhang@jlu.edu.cn.
Gang
G
Zhang
Department of Polymer Science, College of Chemistry, Jilin University, Changchun 130012, China. chemjtang@jlu.edu.cn.
Jun
J
Tang
Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China. quanshun@jlu.edu.cn.
Quanshun
Q
Li
D004798:Enzymes / Q000138:chemical synthesis / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D004853:Epoxy Resins / Q000737:chemistry; D004950:Esterases / Q000737:chemistry*; D007700:Kinetics; D011091:Polyesters / Q000138:chemical synthesis / Q000737:chemistry*; D013696:Temperature
D004798:Enzymes; D004800:Enzymes, Immobilized; D004853:Epoxy Resins; D011091:Polyesters; C016240:polycaprolactone; D004950:Esterases
epoxy support; immobilization; poly(ε-caprolactone); ring-opening polymerization; thermophilic esterase