Green coconut mesocarp pretreated by an alkaline process as raw material for bioethanol production.
Cocos nucifera L., coconut, is a palm of high importance in the food industry, but a considerable part of the biomass is inedible. In this study, the pretreatment and saccharification parameters NaOH solution, pretreatment duration and enzyme load were evaluated for the production of hydrolysates from green coconut mesocarp using 18% (w/v) total solids (TS). Hydrolysates were not detoxified in order to preserve sugars solubilized during the pretreatment. Reduction of enzyme load from 15 to 7.5 filter paper cellulase unit (FPU)/g of biomass has little effect on the final ethanol titer. With optimized pretreatment and saccharification, hydrolysates with more than 7% (w/v) sugars were produced in 48h. Fermentation of the hydrolysate using industrial Saccharomyces cerevisiae strains produced 3.73% (v/v) ethanol. Our results showed a simple pretreatment condition with a high-solid load of biomass followed by saccharification and fermentation of undetoxified coconut mesocarp hydrolysates to produce ethanol with high titer.
Núcleo de Biotecnologia, Centro de Ciências da Saúde, Universidade Federal do Espírito Santo, 29040-090 Vitória, Espírito Santo, Brazil.
Jimmy
J
Soares
Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, KU Leuven, Belgium; Department of Molecular Microbiology, VIB, Kasteelpark Arenberg 31, B-3001 Leuven-Heverlee, Flanders, Belgium.
Mekonnen M
MM
Demeke
Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, KU Leuven, Belgium; Department of Molecular Microbiology, VIB, Kasteelpark Arenberg 31, B-3001 Leuven-Heverlee, Flanders, Belgium.
Maria R
MR
Foulquié-Moreno
Laboratory of Enzyme, Fermentation and Brewing Technology, KAHO Sint-Lieven University College, KU Leuven Association, Gebroeders De Smetstraat 1, 9000 Ghent, Flanders, Belgium.
Miet
M
Van de Velde
Laboratory of Enzyme, Fermentation and Brewing Technology, KAHO Sint-Lieven University College, KU Leuven Association, Gebroeders De Smetstraat 1, 9000 Ghent, Flanders, Belgium.
Alex
A
Verplaetse
Núcleo de Biotecnologia, Centro de Ciências da Saúde, Universidade Federal do Espírito Santo, 29040-090 Vitória, Espírito Santo, Brazil.
Antonio Alberto Ribeiro
AA
Fernandes
Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, KU Leuven, Belgium; Department of Molecular Microbiology, VIB, Kasteelpark Arenberg 31, B-3001 Leuven-Heverlee, Flanders, Belgium.
Johan M
JM
Thevelein
Núcleo de Biotecnologia, Centro de Ciências da Saúde, Universidade Federal do Espírito Santo, 29040-090 Vitória, Espírito Santo, Brazil. Electronic address: patricia.fernandes@pq.cnpq.br.
Patricia Machado Bueno
PM
Fernandes
D056804:Biofuels; D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D050260:Carbohydrate Metabolism / Q000187:drug effects; D003059:Cocos / Q000033:anatomy & histology* / Q000187:drug effects*; D004798:Enzymes / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects; D006868:Hydrolysis; D011208:Powders; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism; D012972:Sodium Hydroxide / Q000494:pharmacology*; D013997:Time Factors
D056804:Biofuels; D004798:Enzymes; D011208:Powders; D000431:Ethanol; D012972:Sodium Hydroxide
Alkaline pretreatment; Green coconut; High-solid load; Mesocarp; Non-detoxified hydrolysate; Second-generation bioethanol