Parameters of oxidative stress in saliva from patients with aggressive and chronic periodontitis.
OBJECTIVE
Free radicals play an important role in the onset and progression of many diseases. The aim of this study was to investigate the contribution of oxidative stress in the pathology of aggressive (AgP) and chronic (CP) periodontitis and its relation with the clinical periodontal status.


METHODS
Eighty subjects were divided into two groups: 20 patients with AgP and 20 patients with CP with their 20 corresponding matched controls, based on clinical attachment loss (CAL), probing pocket depth (PPD), and bleeding on probing (BOP). Saliva reactive oxygen species (ROS), lipid peroxidation, and non-enzymatic antioxidant defences were measured by luminol-dependent chemiluminescence assay, as thiobarbituric acid-reactive substances (TBARs) and total radical-trapping antioxidant potential (TRAP), respectively. Pearson's correlation and multivariate analysis were used to determine the relationship between ROS and TBARs and the clinical parameters.


RESULTS
ROS and TBARs were increased in AgP while TRAP was decreased, comparing with CP. In AgP, a strong and positive correlation was observed between ROS and TBARs and they were closely associated with CAL and PPD.


CONCLUSIONS
In AgP, but not in CP, oxidative stress is a high contributor to periodontal pathology and it is closely associated with the clinical periodontal status.
a Department of Pharmacology, Faculty of Dentistry , University of Buenos Aires , Argentina.|b INBIOMED, National Scientific and Technical Research Council , Argentina.
Andrea B
AB
Acquier
a Department of Pharmacology, Faculty of Dentistry , University of Buenos Aires , Argentina.
Alejandra K
AK
De Couto Pita
a Department of Pharmacology, Faculty of Dentistry , University of Buenos Aires , Argentina.
Lucila
L
Busch
c Biophysics Department, Faculty of Dentistry , University of Buenos Aires , Argentina.
Gabriel A
GA
Sánchez
D000293:Adolescent; D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D016022:Case-Control Studies; D055113:Chronic Periodontitis / Q000145:classification* / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012463:Saliva / Q000737:chemistry* / Q000378:metabolism; D055815:Young Adult
D000975:Antioxidants; D017382:Reactive Oxygen Species
Aggressive periodontitis; Chronic periodontitis; Oxidative stress; ROS; TBARs; TRAP