A novel strategy for highly efficient isolation and analysis of circulating tumor-specific cell-free DNA from lung cancer patients using a reusable conducting polymer nanostructure.
We have developed a reusable nanostructured polypyrrole nanochip and demonstrated its use in the electric field-mediated recovery of circulating cell-free DNA (cfDNA) from the plasma of lung cancer patients. Although cfDNA has been recognized and widely studied as a versatile and promising biomarker for the diagnosis and prognosis of cancers, the lack of efficient strategies to directly isolate cfDNA from the plasma has become a great hindrance to its potential clinical use. As a proof-of-concept study, we demonstrated a technique for the rapid and efficient isolation of cfDNA with high yield and purity. In particular, the synergistic effects of the electro-activity and the nanostructured features of the polypyrrole polymer enabled repeated retrieval of cfDNA using a single platform. Moreover, polypyrrole nanochip facilitated the amplification of tumor-specific DNA fragments from the plasma samples of patients with lung cancer characterized by mutations in exons 21 of the epidermal growth factor receptor gene (EGFR). Overall, the proposed polypyrrole nanochip has enormous potential for industrial and clinical applications with significantly enhanced efficiency in the recovery of tumor-associated circulating cfDNA. This may ultimately contribute to more robust and reliable evaluation of gene mutations in peripheral blood.
New Experimental Therapeutic Branch, National Cancer Center, 323 Ilsan-ro, Ilsandong-gu, Goyang-si, Gyeonggi-do 10408, South Korea; Department of Medical Science, Yonsei University College of Medicine, 50 Yonsei-Ro, Seodaemun-Gu, Seoul 03722, South Korea.
HyungJae
H
Lee
New Experimental Therapeutic Branch, National Cancer Center, 323 Ilsan-ro, Ilsandong-gu, Goyang-si, Gyeonggi-do 10408, South Korea; Global Research Laboratory, Department of Biochemistry and Molecular Biology, Korea University College of Medicine, 73 Inchon-ro, Seongbuk-gu, Seoul 02841, South Korea.
SeungHyun
S
Jeon
Department of Medical Science, Yonsei University College of Medicine, 50 Yonsei-Ro, Seodaemun-Gu, Seoul 03722, South Korea.
Jin-Suck
JS
Seo
Cancer Genomics Branch, National Cancer Center, 323 Ilsan-ro, Ilsandong-gu, Goyang-si, Gyeonggi-do 10408, South Korea.
Sung-Ho
SH
Goh
Lung Cancer Branch, National Cancer Center, 323 Ilsan-ro, Ilsandong-gu, Goyang-si, Gyeonggi-do 10408, South Korea.
Ji-Youn
JY
Han
New Experimental Therapeutic Branch, National Cancer Center, 323 Ilsan-ro, Ilsandong-gu, Goyang-si, Gyeonggi-do 10408, South Korea. Electronic address: yncho@ncc.re.kr.
Youngnam
Y
Cho
D002460:Cell Line; D045744:Cell Line, Tumor; D000074141:Circulating Tumor DNA / Q000097:blood / Q000235:genetics* / Q000302:isolation & purification*; D004867:Equipment Design; D066246:ErbB Receptors / Q000235:genetics; D005091:Exons; D018773:Genes, erbB-1; D006801:Humans; D056656:Lab-On-A-Chip Devices; D008175:Lung Neoplasms / Q000097:blood* / Q000235:genetics*; D049329:Nanostructures / Q000737:chemistry*; D017354:Point Mutation; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*
D000074141:Circulating Tumor DNA; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D066246:ErbB Receptors
Cancer; Circulating cell-free DNA; Conducting polymer; Liquid biopsy; Lung cancer; Mutation detection