Evaluation and characterization of plasmids carrying CTX-M genes in a non-clonal population of multidrug-resistant Enterobacteriaceae isolated from poultry in Brazil.
The increasing presence of ESBL-producing bacteria in food-producing animals might impact on public health. In this study, ESBL-producing enterobacteria were investigated in the microbiota of chickens produced in Brazil. We detected blaCTX-M-2, blaCTX-M-8 and blaCTX-M-15 in 13 Escherichia coli isolates, within 9 different PFGE-types. Escherichia fergusonii and Klebsiella pneumoniae were found carrying blaCTX-M-2. Plasmid Inc groups found included repF, FIB, FIC, I1, Y, B/O, A/C, K and HI1. F plasmids were present in 87.5% of the isolates, however, no resistance gene was harbored in this replicon. The pMLST for IncI1 showed ST113 and the novel ST130, ST131 and ST132 harboring blaCTX-M-8. IncK plasmids carried blaCTX-M-2 in one E. coli isolate. Non-typeable plasmids carrying blaCTX-M-2 or blaCTX-M-15 had up to 260kb. blaCTX-M-2 was also associated with class I integron and ISCR1 and blaCTX-M-8 with IS10. Overall, similar resistance elements were disseminated among a diverse population of ESBL-producing enterobacteria.
School of Pharmaceutical Sciences of Ribeirão Preto - University of São Paulo (USP), Ribeirão Preto, SP, 14040-903, Brazil.
Joseane Cristina
JC
Ferreira
School of Agricultural and Veterinary Sciences - São Paulo State University (UNESP), Jaboticabal, SP, 14884-900, Brazil.
Rafael Antonio Casarin
RA
Penha Filho
School of Pharmaceutical Sciences of Ribeirão Preto - University of São Paulo (USP), Ribeirão Preto, SP, 14040-903, Brazil.
Leonardo Neves
LN
Andrade
School of Agricultural and Veterinary Sciences - São Paulo State University (UNESP), Jaboticabal, SP, 14884-900, Brazil.
Angelo
A
Berchieri Junior
School of Pharmaceutical Sciences of Ribeirão Preto - University of São Paulo (USP), Ribeirão Preto, SP, 14040-903, Brazil. Electronic address: aldarini@fcfrp.usp.br.
Ana Lúcia Costa
AL
Darini
D000818:Animals; D001938:Brazil / Q000453:epidemiology; D002645:Chickens; D016521:Electrophoresis, Gel, Pulsed-Field; D004756:Enterobacteriaceae Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D004926:Escherichia coli / Q000145:classification / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D007711:Klebsiella pneumoniae / Q000145:classification / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D058885:Multilocus Sequence Typing; D010957:Plasmids / Q000032:analysis* / Q000145:classification; D011201:Poultry Diseases / Q000453:epidemiology / Q000382:microbiology*; D001618:beta-Lactamases / Q000235:genetics*
D001618:beta-Lactamases
E. coli; ESBL; Plasmids; antimicrobial resistance; pMLST