Analysis of the sexual development-promoting region of Schizophyllum commune TRP1 gene.
This study aims to elucidate the mechanism of sexual development of basidiomycetous mushrooms from mating to fruit body formation. Sequencing analysis showed the TRP1 gene of basidiomycete Schizophyllum commune encoded an enzyme with three catalytic regions of GAT (glutamine amidotransferase), IGPS (indole-3-glycerol phosphate synthase), and PRAI (5-phosphoribosyl anthranilate isomerase); among these three regions, the trp1 mutant (Trp(-)) had a missense mutation (L→F) of a 338th amino acid residue of the TRP1 protein within the IGPS region. To investigate the function of IGPS region related to sexual development, dikaryons with high, usual, and no expression of the IGPS region of TRP1 gene were made. The dikaryotic mycelia with high expression of the IGPS formed mature fruit bodies earlier than those with usual and no expression of the IGPS. These results showed that the IGPS region in TRP1 gene promoted sexual development of S. commune.
a Faculty of Agriculture, Division of Bioscience and Biotechnology, Department of Agricultural and Life Sciences , Shinshu University , Nagano , Japan.
Kikuo
K
Sen
a Faculty of Agriculture, Division of Bioscience and Biotechnology, Department of Agricultural and Life Sciences , Shinshu University , Nagano , Japan.
Hideki
H
Kinoshita
a Faculty of Agriculture, Division of Bioscience and Biotechnology, Department of Agricultural and Life Sciences , Shinshu University , Nagano , Japan.
Kazuyuki
K
Tazuke
a Faculty of Agriculture, Division of Bioscience and Biotechnology, Department of Agricultural and Life Sciences , Shinshu University , Nagano , Japan.
Yoshinori
Y
Maki
a Faculty of Agriculture, Division of Bioscience and Biotechnology, Department of Agricultural and Life Sciences , Shinshu University , Nagano , Japan.
Yumi
Y
Yoshiura
b Faculty of Agriculture, Department of Biological Chemistry , Yamaguchi University , Yamaguchi , Japan.
Toshiharu
T
Yakushi
a Faculty of Agriculture, Division of Bioscience and Biotechnology, Department of Agricultural and Life Sciences , Shinshu University , Nagano , Japan.
Hiroshiro
H
Shibai
a Faculty of Agriculture, Division of Bioscience and Biotechnology, Department of Agricultural and Life Sciences , Shinshu University , Nagano , Japan.
Shin-Ichi
S
Kurosawa
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D001483:Base Sequence; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D007210:Indoleacetic Acids / Q000494:pharmacology; D007211:Indoles / Q000494:pharmacology; D009154:Mutation; D017433:Protein Structure, Secondary; D012567:Schizophyllum / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D016415:Sequence Alignment; D014158:Transcription, Genetic; D014364:Tryptophan / Q000494:pharmacology
D005656:Fungal Proteins; D007210:Indoleacetic Acids; D007211:Indoles; C030737:indoleacetic acid; C030374:indole; D014364:Tryptophan
Schizophyllum commune; TRP1; fruit body formation; indole-3-glycerol phosphate synthase; sexual development