Extracellular space diffusion analysis in the infant and adult rat striatum using magnetic resonance imaging.
The extracellular space (ECS) in the brain provides an extrasynaptic transfer channel among neurons, axons and glial cells. It is particularly important in the early stage after birth, when angiogenesis is not yet complete and the ECS may provide the main pathway for metabolite transport. However, the characteristics of extracellular transport remain unclear. In this study, a novel magnetic resonance imaging (MRI) method was used to perform real-time visualization and quantification of diffusion in the brain ECS of infant (postnatal day 10 (P10)) and adult rats. Using a modified diffusion equation and the linear relationship between the signal intensity and the gadolinium-diethylenetriaminepentaacetic acid (Gd-DTPA) concentration, diffusion parameters were obtained; these parameters include the effective diffusion coefficient (D*), clearance rate (k'), tortuosity (λ) and the volume fraction of distribution (Vd%). There were significant differences in the diffusion parameters between P10 and adult rats. This finding provides a reference for future treatment of brain diseases using drugs administered via interstitial pathways.
Imaging Center, Beijing Children's Hospital, Capital Medical University, Beijing 100045, China.
Shuangfeng
S
Yang
Imaging Center, Beijing Children's Hospital, Capital Medical University, Beijing 100045, China.
Yan
Y
Wang
Department of Radiology, Beijing Jishuitan Hospital, Beijing 100035, China.
Kai
K
Li
Imaging Center, Beijing Children's Hospital, Capital Medical University, Beijing 100045, China.
Xiaolu
X
Tang
Department of Laboratory Animal Science, Peking University Health Science Center, Beijing 100191, China.
Kuo
K
Zhang
Department of Radiology, Peking University Third Hospital, Beijing 100191, China; Beijing Key Lab of Magnetic Resonance Imaging Device and Technique, Beijing 100191, China.
Chunyan
C
Shi
Department of Radiology, Peking University Third Hospital, Beijing 100191, China; Beijing Key Lab of Magnetic Resonance Imaging Device and Technique, Beijing 100191, China. Electronic address: hanhongbin@bjmu.edu.cn.
Hongbin
H
Han
Imaging Center, Beijing Children's Hospital, Capital Medical University, Beijing 100045, China. Electronic address: ppengyun@yahoo.com.
Yun
Y
Peng
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D015816:Cell Adhesion Molecules, Neuronal / Q000378:metabolism; D003342:Corpus Striatum / Q000166:cytology* / Q000254:growth & development* / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005110:Extracellular Space / Q000000981:diagnostic imaging* / Q000378:metabolism; D005215:Fasting; D019786:Gadolinium DTPA / Q000493:pharmacokinetics; D008279:Magnetic Resonance Imaging; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D000091042:Reelin Protein; D012697:Serine Endopeptidases / Q000378:metabolism; D019063:Tenascin / Q000378:metabolism; D013997:Time Factors; D014868:Water Deprivation
D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; D009419:Nerve Tissue Proteins; D000091042:Reelin Protein; D019063:Tenascin; D012697:Serine Endopeptidases; D019786:Gadolinium DTPA
Development; Diffusion; Extracellular space; Gd-DTPA; Magnetic resonance imaging