Surface plasmon resonance biosensor for detection of pregnancy associated plasma protein A2 in clinical samples.
Pregnancy associated plasma protein A2 (PAPP-A2) is a metalloproteinase that plays multiple roles in fetal development and post-natal growth. Here we present a novel surface plasmon resonance (SPR) biosensor for the rapid and quantitative detection of PAPP-A2 in blood samples. This biosensor uses a single surface referencing approach and a sandwich assay with functionalized gold nanoparticles for signal enhancement. We demonstrate that this SPR biosensor enables the detection of PAPP-A2 in 30 % blood plasma at levels as low as 3.6 ng/mL. We also characterize the performance of the biosensor and evaluate its cross-reactivity to a PAPP-A analogue. Finally, we utilize this SPR biosensor for the detection of PAPP-A2 in blood serum from two groups of subjects: pregnant women and healthy non-pregnant women and men. Graphical Abstract Temporal sensor response corresponding to respective steps of the assay for detection of PAPP-A2 in buffer.
Department of Optical Sensors, Institute of Photonics and Electronics of the CAS, Chaberska 57, 18251, Prague 8, Czech Republic.
Markéta
M
Bocková
Department of Optical Sensors, Institute of Photonics and Electronics of the CAS, Chaberska 57, 18251, Prague 8, Czech Republic.
Xue
X
Chadtová Song
Department of Optical Sensors, Institute of Photonics and Electronics of the CAS, Chaberska 57, 18251, Prague 8, Czech Republic.
Erika
E
Gedeonová
Institute of Medical Biochemistry and Laboratory Diagnostics of First Faculty of Medicine, Charles University in Prague and the General University Hospital in Prague, U Nemocnice 2, 12808, Praha 2, Czech Republic.
Kateřina
K
Levová
Institute of Medical Biochemistry and Laboratory Diagnostics of First Faculty of Medicine, Charles University in Prague and the General University Hospital in Prague, U Nemocnice 2, 12808, Praha 2, Czech Republic.
Marta
M
Kalousová
Institute of Medical Biochemistry and Laboratory Diagnostics of First Faculty of Medicine, Charles University in Prague and the General University Hospital in Prague, U Nemocnice 2, 12808, Praha 2, Czech Republic.
Tomáš
T
Zima
Department of Optical Sensors, Institute of Photonics and Electronics of the CAS, Chaberska 57, 18251, Prague 8, Czech Republic. homola@ufe.cz.
Jiří
J
Homola
D002021:Buffers; D005260:Female; D006046:Gold / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry; D011247:Pregnancy; D011266:Pregnancy-Associated Plasma Protein-A / Q000032:analysis*; D020349:Surface Plasmon Resonance / Q000379:methods*
D002021:Buffers; D006046:Gold; C430889:PAPPA2 protein, human; D011266:Pregnancy-Associated Plasma Protein-A
Blood sample; Nanoparticles; Pregnancy associated plasma protein A2; Surface plasmon resonance