MIL-53(Fe) MOF-mediated catalytic chemiluminescence for sensitive detection of glucose.
Various analytical applications of metal-organic frameworks (MOFs) have been rapidly developed in the past few years. However, the employment of MOFs as catalysts in chemiluminescence (CL) analysis is rare. Here, for the first time, we found that MIL-53(Fe) MOFs could significantly enhance the CL of luminol in the presence of H2O2in an alkaline medium. The CL intensity in the luminol-H2O2-MIL-53(Fe) system was about 20 times higher than that in the luminol-H2O2system. Moreover, the XRD pattern of MIL-53(Fe) after CL reaction was almost the same as that of the original MIL-53(Fe), confirming the catalytic role of MIL-53(Fe) in the luminol-H2O2-MIL-53(Fe) system. The possible mechanism behind the enhancing phenomenon was discussed based on the results from the CL spectra, FL probe experiments, and active oxygen species measurements. By coupling with the glucose oxidase-based catalytic oxidation reaction, a sensitive and selective CL method was developed for the detection of glucose. There is a linear relationship between the logarithm of CL intensity and the logarithm of glucose concentration in the range from 0.1 to 10 μM, and a detection limit of 0.05 μM (S/N = 3) is obtained. The proposed method has been applied to the determination of glucose in human serum samples with satisfactory results. Graphical abstract MIL-53(Fe) MOFs are found to greatly enhance the chemiluminescence emission of the luminol-H2O2system, and this finding resulted in a new chemiluminescence method for biosensing of glucose when coupled with the glucose oxidase.
The Key Laboratory of Luminescence and Real-Time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Beibei, Chongqing, 400715, China.
Xueling
X
Yi
The Key Laboratory of Luminescence and Real-Time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Beibei, Chongqing, 400715, China.
Wenfei
W
Dong
The Key Laboratory of Luminescence and Real-Time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Beibei, Chongqing, 400715, China.
Xiaodan
X
Zhang
College of Resources and Environment, Yuxi Normal University, Yuxi, Yunnan, 653100, China. xjx11228@163.com.
Jianxin
J
Xie
The Key Laboratory of Luminescence and Real-Time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Beibei, Chongqing, 400715, China. yuminghuang2000@yahoo.com.
Yuming
Y
Huang
D001681:Biological Assay; D001786:Blood Glucose / Q000032:analysis*; D002384:Catalysis; D005949:Glucose Oxidase / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry*; D057230:Limit of Detection; D049449:Luminescence; D008163:Luminescent Measurements; D008165:Luminol / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry*; D010084:Oxidation-Reduction
D001786:Blood Glucose; D009942:Organometallic Compounds; D008165:Luminol; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase
Chemiluminescence; Glucose; MIL-53(Fe); Metal–organic frameworks