Mitotic stopwatch for the blast fungus Magnaporthe oryzae during invasion of rice cells.
To study nuclear dynamics of Magnaporthe oryzae, we developed a novel mitotic reporter strain with GFP-NLS (localized in nuclei during interphase but in the cytoplasm during mitosis) and H1-tdTomato (localized in nuclei throughout the cell cycle). Time-lapse confocal microscopy of the reporter strain during host cell invasion provided several new insights into nuclear division and migration in M. oryzae: (i) mitosis lasts about 5min; (ii) mitosis is semi-closed; (iii) septal pores are closed during mitosis; and (iv) a nucleus exhibits extreme constriction (approximately from 2μm to 0.5μm), elongation (over 5μm), and long migration (over 16μm). Our observations raise new questions about mechanisms controlling the mitotic dynamics, and the answers to these questions may result in new means to prevent fungal proliferation without negatively affecting the host cell cycle.
Department of Plant Biology, University of Georgia, Athens 30602, USA.
Kiersun
K
Jones
Department of Plant Biology, University of Georgia, Athens 30602, USA.
Cory B
CB
Jenkinson
Department of Plant Biology, University of Georgia, Athens 30602, USA.
Maíra
M
Borges Araújo
Department of Plant Biology, University of Georgia, Athens 30602, USA.
Jie
J
Zhu
Department of Plant Biology, University of Georgia, Athens 30602, USA.
Rebecca Y
RY
Kim
Department of Plant Biology, University of Georgia, Athens 30602, USA.
Dong Won
DW
Kim
Department of Plant Biology, University of Georgia, Athens 30602, USA. Electronic address: ckhang@uga.edu.
Chang Hyun
CH
Khang
D002467:Cell Nucleus / Q000235:genetics; D003593:Cytoplasm / Q000235:genetics / Q000382:microbiology; D020082:Magnaporthe / Q000235:genetics* / Q000472:pathogenicity; D008938:Mitosis / Q000235:genetics*; D012275:Oryza / Q000382:microbiology*; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology
Cell cycle; Open mitosis; Time-lapse confocal microscopy