Application of Fluorescence Polarization in HTS Assays.
Steady-state measurements of fluorescence polarization have been widely adopted in the field of high-throughput screening for the study of biomolecular interactions. This chapter reviews the basic theory of fluorescence polarization, the underlying principle for using fluorescence polarization to study interactions between small-molecule fluorophores and macromolecular targets, and representative applications of fluorescence polarization in high-throughput screening.
Immunology, Inflammation and Infectious Diseases Discovery and Translational Area Roche Pharma Research & Early Development, Roche Innovation Center Shanghai, Roche R&D Center (China) Ltd, 720 Cai Lun Road, Bldg 5, Pudong, Shanghai, 201203, China. xinyi.huang@roche.com.
Xinyi
X
Huang
Immunology, Inflammation and Infectious Diseases Discovery and Translational Area Roche Pharma Research & Early Development, Roche Innovation Center Shanghai, Roche R&D Center (China) Ltd, 720 Cai Lun Road, Bldg 5, Pudong, Shanghai, 201203, China.
Ann
A
Aulabaugh
D000818:Animals; D016880:Anisotropy; D001667:Binding, Competitive; D057075:Enzyme Assays / Q000379:methods*; D005454:Fluorescence Polarization / Q000379:methods*; D005456:Fluorescent Dyes / Q000032:analysis* / Q000378:metabolism; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans
D005456:Fluorescent Dyes
Anisotropy; Competition binding; FP; High-throughput screening; Polarization