Dietary glycerol for quail: association between productive performance and COX III mRNA expression.
This study was carry out to evaluate mRNA expression of mitochondrial cytochrome c oxidase III in the Pectoralis superficialis muscle of 28-day-old quails fed diets containing 0, 8, and 12% glycerol. Total RNA was extracted (N = 10) and cDNA was amplified using specifics primers for qRT-PCR. Feed efficiency and feed intake were evaluated. COX III mRNA expression in breast muscle was higher in the group fed with 12% glycerol (0.863 AU); no differences were observed in the expression of this gene between the muscle of animals fed diets without glycerol (0.357 AU) and 8% glycerol (0.415 AU). Quails that showed greater COX III mRNA expression also showed the lowest feed efficiency. These results show that there is a difference in COX III mRNA expression in breast muscle of 28-day-old quail fed diets different concentrations of glycerol.
Departamento de Medicina Veterinária, Centro Universitário de Maringá, Maringá, PR, Brasil.
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SC
Silva
Programa de Pós-Graduação em Zootecnia, Universidade Estadual de Maringá, Maringá, PR, Brasil.
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E
Gasparino
Programa de Pós-Graduação em Zootecnia, Universidade Estadual de Maringá, Maringá, PR, Brasil.
E
E
Batista
Programa de Pós-Graduação em Zootecnia, Universidade Estadual Paulista, Jaboticabal, SP, Brasil.
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Tanamati
Departamento de Zootecnia, Universidade Federal de Sergipe, São Cristóvão, SE, Brasil.
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Vesco
Programa de Pós-Graduação em Zootecnia, Universidade Estadual de Maringá, Maringá, PR, Brasil.
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B
Lala
Programa de Pós-Graduação em Zootecnia, Universidade Estadual Paulista, Jaboticabal, SP, Brasil.
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DP
de Oliveira
D000821:Animal Feed; D000818:Animals; D004032:Diet; D003576:Electron Transport Complex IV / Q000096:biosynthesis* / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D005990:Glycerol / Q000008:administration & dosage*; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D011784:Quail / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
D012333:RNA, Messenger; D003576:Electron Transport Complex IV; D005990:Glycerol