First Identification of Chlamydia psittaci in the Acute Illness and Death of Endemic and Endangered Psittacine Birds in Mexico.
A mortality episode of endemic and endangered psittacine birds from the genera Ara and Amazona occurred during January 2015. The birds were housed in a management unit for wildlife conservation that receives wild-caught birds from illegal trade. In total, 11 (57%) adult birds of different origins that shared these accommodations died. Only four of them were sent for diagnosis. The main lesions found at necropsy were consistent with those described previously for avian chlamydiosis; the presence of Chlamydiaceae was confirmed through immunofluorescence and amplification with further sequencing of the 16S ribosomal RNA gene by using hepatic tissue. Due to the lack of specific diagnostic tools on primary psittacine diseases, the pathogenic effects of systemic, respiratory, or enteric infections with high mortality rates remain unknown in Mexico. In this study, specific molecular identification of avian chlamydiosis was performed using a nested PCR on liver tissues, as well as choanal and cloacal swab samples, confirming the presence of Chlamydia psittaci in all of them. In addition, it was possible to obtain the ompA gene sequence from processed clinical samples, thereby allowing us to determine that the A genotype was affecting these birds. Although this genotype is the most commonly found worldwide in psittacine birds, this case report describes the first avian chlamydiosis outbreak affecting critically endangered and endemic psittacines subjected to reintegration programs in Mexico. Consequently, this study demonstrates the necessity of more exhaustive biosecurity strategies because other pathogens may be present and should be assessed, especially in highly threatened birds, before releasing them into their habitats.
A Department of Avian Medicine and Poultry Husbandry, Faculty of Veterinary Medicine and Animal Production, National Autonomous University of Mexico, Coyoacan 04510, D.F., Mexico.
E
E
Ornelas-Eusebio
A Department of Avian Medicine and Poultry Husbandry, Faculty of Veterinary Medicine and Animal Production, National Autonomous University of Mexico, Coyoacan 04510, D.F., Mexico.
F D
FD
Sánchez-Godoy
A Department of Avian Medicine and Poultry Husbandry, Faculty of Veterinary Medicine and Animal Production, National Autonomous University of Mexico, Coyoacan 04510, D.F., Mexico.
F
F
Chávez-Maya
B Department of Ecology of Pathogenic Agents, Dr. Manuel Gea González General Hospital, Tlalpan 14080, D.F., Mexico.
J A
JA
De la Garza-García
B Department of Ecology of Pathogenic Agents, Dr. Manuel Gea González General Hospital, Tlalpan 14080, D.F., Mexico.
R
R
Hernández-Castro
A Department of Avian Medicine and Poultry Husbandry, Faculty of Veterinary Medicine and Animal Production, National Autonomous University of Mexico, Coyoacan 04510, D.F., Mexico.
G
G
García-Espinosa
D000208:Acute Disease; D000818:Animals; D000836:Animals, Zoo; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001715:Bird Diseases / Q000175:diagnosis / Q000453:epidemiology* / Q000382:microbiology; D002691:Chlamydophila psittaci / Q000235:genetics / Q000302:isolation & purification*; D004196:Disease Outbreaks / Q000662:veterinary*; D056727:Endangered Species; D008800:Mexico / Q000453:epidemiology; D010311:Parrots; D009956:Psittacosis / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D017422:Sequence Analysis, DNA / Q000662:veterinary
D001425:Bacterial Outer Membrane Proteins; C078167:OMPA outer membrane proteins
Chlamydia pisttaci; Mexico; acute disease; endemic psittacine; genotype A