Activation of macrophages by the ophiopogon polysaccharide liposome from the root tuber of Ophiopogon japonicus.
This study evaluated the immunomodulatory effects of ophiopogon polysaccharide liposome (OPL) on macrophages in vitro. The phagocytic activity, the secretion of nitric oxide (NO) and inducible nitric oxide synthase (iNOS), the level of cytokines, chemokines and the expression of CD14 and MHC-II costimulatory molecules were measured. Results showed that OPL could significantly improve the phagocytic activity and the level of IL-1β, TNF-α, MCP-1 and MIP-1β, promote the secretion of NO and iNOS, and enhance the expression of CD14 and MHC-II costimulatory molecules in the peritoneal macrophages of mice compared with ophiopogon polysaccharide (OP). Altogether, these results suggested that OPL could activate macrophages, and the efficacy was significantly superior to OP. Therefore, OPL would be exploited in a potent immunomodulators. Moreover, it also provided the theoretical basis for further studying the mechanism of OPL on improving the immune response.
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
Wenjing
W
Sun
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
Wenjie
W
Hu
Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu, 210014, PR China.
Kai
K
Meng
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
Lumiao
L
Yang
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
Weimin
W
Zhang
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
Xiaoping
X
Song
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
Xiaohao
X
Qu
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
Yueyang
Y
Zhang
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
Lin
L
Ma
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China. Electronic address: ypfan@nwsuaf.edu.cn.
Yunpeng
Y
Fan
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008081:Liposomes / Q000494:pharmacology*; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D008813:Mice, Inbred ICR; D009569:Nitric Oxide / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D031441:Ophiopogon / Q000737:chemistry*; D010587:Phagocytosis / Q000187:drug effects; D018517:Plant Roots / Q000737:chemistry*; D035281:Plant Tubers / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*
D016207:Cytokines; D000949:Histocompatibility Antigens Class II; D018950:Lipopolysaccharide Receptors; D008081:Liposomes; D011134:Polysaccharides; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II
Ophiopogon polysaccharide liposome; Peritoneal macrophages; Phagocytosis