Ultraviolet-Visible (UV-Vis) and Fluorescence Spectroscopic Investigation of the Interactions of Ionic Liquids and Catalase.
The inhibitory effects of nine ionic liquids (ILs) on the catalase activity were investigated using fluorescence, absorption ultraviolet-visible spectroscopy. The interactions of ILs and catalase on the molecular level were studied. The experimental results indicated that ILs could inhibit the catalase activity and their inhibitory abilities depended on their chemical structures. Fluorescence experiments showed that hydrogen bonding played an important role in the interaction process. The inhibitory abilities of ILs on catalase activity could be simply described by their hydrophobicity and hydrogen bonding abilities. Unexpected less inhibitory effects of trifluoromethanesulfonate (TfO-) might be ascribed to its larger size, which makes it difficult to go through the substrate channel of catalase to the active site.
College of Physics and Chemistry, Henan Polytechnic University, China.
Xing
X
Dong
College of Physics and Chemistry, Henan Polytechnic University, China yunchangfan2009@163.com.
Yunchang
Y
Fan
College of Physics and Chemistry, Henan Polytechnic University, China.
Peng
P
Yang
College of Physics and Chemistry, Henan Polytechnic University, China.
Jichuan
J
Kong
College of Physics and Chemistry, Henan Polytechnic University, China.
Dandan
D
Li
College of Physics and Chemistry, Henan Polytechnic University, China.
Juan
J
Miao
College of Physics and Chemistry, Henan Polytechnic University, China.
Shaofeng
S
Hua
College of Physics and Chemistry, Henan Polytechnic University, China.
Chaobing
C
Hu
D000818:Animals; D002374:Catalase / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D002417:Cattle; D006860:Hydrogen Bonding; D057927:Hydrophobic and Hydrophilic Interactions; D052578:Ionic Liquids / Q000737:chemistry / Q000494:pharmacology; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D052578:Ionic Liquids; D002374:Catalase
ILs; Ionic liquids; catalase; fluorescence spectroscopy; hydrogen bonding; hydrophobicity