Transforming growth factor-β1 induces fibrosis in rat meningeal mesothelial cells via the p38 signaling pathway.
Leptomeningeal fibrosis is important in the pathogenesis of communicating hydrocephalus following subarachnoid hemorrhage; however, the underlying mechanisms of leptomeningeal fibrosis remain largely unclear. In the present study, primary meningeal mesothelial cells (MMCs) were used as a cell model to investigate the effect of transforming growth factor‑β1 (TGF‑β1) on leptomeningeal fibrosis. Firstly, primary MMCs were isolated from rat brains and characterized by immunofluorescene, staining positive for keratin and vimentin, but negative for factor VIII. Upon TGF‑β1 treatment, MMCs were induced to express connective tissue growth factor (CTGF), an indicator of fibrosis, in a dose‑dependent manner. Furthermore, p38 mitogen‑activated protein kinase (MAPK) signaling was significantly activated by TGF‑β1. However, in the presence of a p38 MAPK inhibitor, TGF‑β1‑induced CTGF expression was markedly suppressed. Together, these data suggest that TGF‑β1 could induce fibrosis of MMCs via the p38 MAPK signaling pathway, providing a novel potential target for intervention in TGF‑β1‑induced leptomeningeal fibrosis.
Department of Neurology, First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450003, P.R. China.
Xue-Jing
XJ
Yue
Department of Neurology, First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453100, P.R. China.
Yan
Y
Guo
College of Life Science and Technology, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.
Hai-Jie
HJ
Yang
College of Life Science and Technology, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China.
Zhi-Wei
ZW
Feng
Department of Neurology, First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453100, P.R. China.
Tong
T
Li
Department of Neurology, First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450003, P.R. China.
Yu-Ming
YM
Xu
D000818:Animals; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology*; D005355:Fibrosis; D008578:Meninges / Q000378:metabolism* / Q000473:pathology*; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects; D053773:Transforming Growth Factor beta1 / Q000378:metabolism* / Q000494:pharmacology; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
D053773:Transforming Growth Factor beta1; D048051:p38 Mitogen-Activated Protein Kinases