Interactions between Bacillus cereus CGMCC 1.895 and Clostridium beijerinckii NCIMB 8052 in coculture for butanol production under nonanaerobic conditions.
Low oxygen tolerance and substrate restriction continues to hamper the process of biobutanol industrialization. In this work, butanol fermentation with cocultures of Bacillus cereus China General Microbiological Culture Collection Center (CGMCC) 1.895 and Clostridium beijerinckii NCIMB 8052 under nonanaerobic conditions was investigated, and the interactions between these two strains were examined. The addition of B. cereus CGMCC 1.895 resulted in higher oxygen tolerance and a wider range of substrate utilization, compared with the pure culture of C. beijerinckii NCIMB 8052. Butanol concentration reached 10.49 g/L with an optimized inoculation size of 90% under nonanaerobic conditions, and this concentration was close to that of pure C. beijerinckii NCIMB 8052 culture under anaerobic conditions. Dynamic relative abundance analysis demonstrated that the ratio of C. beijerinckii NCIMB 8052 accounted for nearly 99% of the cocultured cells. Furthermore, the substrate utilization range was expanded, allowing the use of corn mash for butanol production. The final concentration of butanol and total solvents was 6.78 and 10.52 g/L, respectively. Coculture also was performed successfully in a 5-L fermenter and 8.75 g/L butanol was obtained. Dynamic dissolved oxygen analysis demonstrated that B. cereus consumed the dissolved oxygen in the broth and resulted in the anaerobic condition for C. beijerinckii.
Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, People's Republic of China.
Shuai
S
Mai
Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, People's Republic of China.
Genyu
G
Wang
Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, People's Republic of China.
Pengfei
P
Wu
Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, People's Republic of China.|Key Laboratory of Carbohydrate Chemistry and Biotechnology, School of Biotechnology, Jiangnan University, Wuxi, People's Republic of China.
Chunkai
C
Gu
Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, People's Republic of China.
Hongjuan
H
Liu
Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, People's Republic of China.
Jianan
J
Zhang
Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, People's Republic of China.
Gehua
G
Wang
D001409:Bacillus cereus / Q000378:metabolism* / Q000502:physiology; D056804:Biofuels; D019149:Bioreactors / Q000382:microbiology*; D000440:Butanols / Q000032:analysis / Q000378:metabolism*; D046970:Clostridium beijerinckii / Q000378:metabolism* / Q000502:physiology; D018920:Coculture Techniques / Q000379:methods*; D005285:Fermentation; D007218:Industrial Microbiology; D010100:Oxygen / Q000378:metabolism
D056804:Biofuels; D000440:Butanols; D010100:Oxygen
biofuels; butanol; coculture; fermentation; interactions; microbial metabolism