Evaluation of diacylphospholipids as boundary lipids for bacteriorhodopsin from structural and functional aspects.
Reconstituted membranes with diverse diacylphospholipids were prepared by using bacteriorhodopsin (bR) in which the intrinsic lipid content was decreased to 24% of the original while the trimeric structure and photocycle of bR were retained. Four phospholipids with a different headgroup, phosphatidic acid (PA), phosphatidylcholine (PC), phosphatidylglycerol (PG), and phosphatidylserine (PS), were adopted for reconstitution. By varying the lipid-protein ratios, the interactions of these phospholipids with bR, as a boundary lipid, were evaluated by solid state (2)H/(31)P NMR, circular dichroism (CD), and laser-flash photolysis. The (31)P NMR results revealed that the headgroup of acidic phosphatidylglycerol (PG) interacts more strongly with bR than that of phosphatidylcholine (PC). CD analysis indicated that the trimetric structure of bR was retained in all the phospholipid-bR preparations at low and medium lipid contents. Acidic lipids PA, PG and PS restored the photocycle activity of bR to an extent comparable to (or slightly lower than) that of the purple membrane while PC caused a marked reduction of the bR photocycle efficiency. Among PGs with different fatty acyl groups, those with mono- and di-unsaturated lipids tended to preserve the photocycle efficiency, whereas the fully saturated lipid did not. These results show that acidic unsaturated phospholipids, particularly dioleoylphosphatidylglycerol (DOPG), have higher affinity for bR and efficiently restore its trimetric structure. The present study suggests that bR reconstituted in DOPG bilayers may possibly be used as a model system for spectroscopic investigations of the lipid-bR interactions with the membrane-integral α-helices, and potentially for a similar type of membrane proteins.
JST ERATO, Lipid Active Structure Project, Osaka University, Osaka, Japan; Department of Chemistry, Graduate School of Science, Osaka University, Osaka, Japan; Project Research Centre for Fundamental Sciences, Osaka University, Osaka, Japan.
Satoshi
S
Kawatake
JST ERATO, Lipid Active Structure Project, Osaka University, Osaka, Japan; Department of Chemistry, Graduate School of Science, Osaka University, Osaka, Japan; Project Research Centre for Fundamental Sciences, Osaka University, Osaka, Japan.
Yuichi
Y
Umegawa
JST ERATO, Lipid Active Structure Project, Osaka University, Osaka, Japan; Department of Chemistry, Graduate School of Science, Osaka University, Osaka, Japan; Project Research Centre for Fundamental Sciences, Osaka University, Osaka, Japan.
Shigeru
S
Matsuoka
JST ERATO, Lipid Active Structure Project, Osaka University, Osaka, Japan; Department of Chemistry, Graduate School of Science, Osaka University, Osaka, Japan. Electronic address: murata@chem.sci.osaka-u.ac.jp.
Michio
M
Murata
Division of Molecular Sciences, Graduate School of Science and Technology, Gunma University, Kiryu, Japan.
Masashi
M
Sonoyama
D001436:Bacteriorhodopsins / Q000737:chemistry*; D002942:Circular Dichroism; D019617:Halobacterium salinarum / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D010715:Phosphatidylglycerols / Q000737:chemistry*; D010782:Photolysis
D008051:Lipid Bilayers; D010715:Phosphatidylglycerols; D001436:Bacteriorhodopsins; C051388:1,2-dioleoyl-sn-glycero-3-phosphoglycerol
Bacteriorhodopsin; Boundary lipid; Lipid-protein interaction; Phosphatidylglycerol; Solid state NMR; Unsaturated acyl groups