Hyaluronan and hyaluronan synthases expression and localization in embryonic mouse molars.
Hyaluronan (HA) and hyaluronan synthases (HASs) have been shown to play critical roles in embryogenesis and organ development. However, there have not been any studies examining HA and HAS expression and localization during tooth development. The present study was designed to investigate the expression of HA and three isoforms of HASs (HAS1, 2, 3) in embryonic mouse molars. The first mandibular embryonic mouse molars were examined by immunohistochemistry at E11.5, E13.5, E14.5, E16.5, and E18.5. PCR and western blot analyses were performed on RNA and proteins samples from E13.5 to E18.5 tooth germs. At the initial stage (E11.5), HA and HASs were expressed in the dental epithelium but not the underlying dental mesenchyme. HA immunostaining gradually increased in the enamel organ from the bud stage (E13.5) to the late bell stage (E18.5), and HA and HASs were highly expressed in the stellate reticulum and stratum intermedium. HA immunostaining was also enhanced in the dental mesenchyme and its derived tissues, but it was not expressed in the ameloblast and odontoblast regions. The three HAS isoforms had distinct expression patterns, and they were expressed in the dental mesenchyme and odontoblast at various levels. Furthermore, HAS1 and HAS2 expression decreased, while HAS3 expression increased from E13.5 to E18.5. These results suggested that HA synthesized by different HASs is involved in embryonic mouse molar morphogenesis and cytodifferentiation.
Department of Endodontics, School & Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, 200072, People's Republic of China.
Guofeng
G
Yang
Department of Endodontics, School & Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, 200072, People's Republic of China. jiangbeizhan@tongji.edu.cn.
Beizhan
B
Jiang
Department of Stomatology, Huashan Hospital, Fudan University, Shanghai, 200040, People's Republic of China.
Wenping
W
Cai
Department of Stomatology, Huashan Hospital, Fudan University, Shanghai, 200040, People's Republic of China.
Shangfeng
S
Liu
Department of Endodontics, School & Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, 200072, People's Republic of China. slzhao@fudan.edu.cn.|Department of Stomatology, Huashan Hospital, Fudan University, Shanghai, 200040, People's Republic of China. slzhao@fudan.edu.cn.
Shouliang
S
Zhao
D000818:Animals; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D014453:Glucuronosyltransferase / Q000235:genetics* / Q000378:metabolism*; D000076002:Hyaluronan Synthases; D006820:Hyaluronic Acid / Q000378:metabolism*; D007150:Immunohistochemistry; D051379:Mice; D008963:Molar / Q000196:embryology* / Q000378:metabolism*; D009805:Odontogenesis / Q000235:genetics; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014083:Tooth Germ / Q000196:embryology / Q000378:metabolism
D012333:RNA, Messenger; D006820:Hyaluronic Acid; D014453:Glucuronosyltransferase; D000076002:Hyaluronan Synthases
Hyaluronan; Hyaluronan synthase; Molar; Mouse; Tooth development; Tooth germ