Effect of Porphyrin Sensitizer MgTPPS4 on Cytoskeletal System of HeLa Cell Line-Microscopic Study.
Metalloporphyrins are an important group of sensitizers with a porphyrin skeleton. Their photophysical properties are significantly affected by the nature of the central ion. In this work, we focus on the mechanical properties of a cervix carcinoma cell line which underwent photodynamic treatment (PDT) with MgTPPS4 photosensitzer. Atomic force microscopy alongside confocal microscopy was used to quantify and qualify the structural characteristics before and after PDT. Cells before PDT showed a fine actin network and higher elasticity with the median of Young modulus 12.2 kPa. After PDT, the median of Young modulus was 13.4 kPa and a large redistribution in the actin network was observed.
Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 3, 77515, Olomouc, Czech Republic. jakub.malohlava@upol.cz.|Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 5, 77515, Olomouc, Czech Republic. jakub.malohlava@upol.cz.
Jakub
J
Malohlava
Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 3, 77515, Olomouc, Czech Republic.|Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 5, 77515, Olomouc, Czech Republic.
Katerina
K
Tomankova
Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 3, 77515, Olomouc, Czech Republic.|Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 5, 77515, Olomouc, Czech Republic.
Lukas
L
Malina
Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 3, 77515, Olomouc, Czech Republic.|Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 5, 77515, Olomouc, Czech Republic.
Jana
J
Jiravova
Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 3, 77515, Olomouc, Czech Republic.|Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 5, 77515, Olomouc, Czech Republic.
Adela
A
Hanakova
Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 3, 77515, Olomouc, Czech Republic.|Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 5, 77515, Olomouc, Czech Republic.
Klara
K
Pizova
Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 3, 77515, Olomouc, Czech Republic.
Jana
J
Zapletalova
Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 3, 77515, Olomouc, Czech Republic.|Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 5, 77515, Olomouc, Czech Republic.
Hana
H
Kolarova
D003599:Cytoskeleton / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects; D055119:Elastic Modulus; D006367:HeLa Cells; D006801:Humans; D008027:Light; D008665:Metalloporphyrins / Q000737:chemistry / Q000494:pharmacology*; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D017319:Photosensitizing Agents / Q000737:chemistry / Q000494:pharmacology*
D008665:Metalloporphyrins; D017319:Photosensitizing Agents; C568228:magnesium 5,10,15,20-tetrakis(4-sulphophenyl)-porphine dodecahydrate
Atomic force microscopy; Cytoskeleton; F-actin; Mechanical properties; Photodynamic treatment