Fabrication, Characterization, and Evaluation of Bionanocomposites Based on Natural Polymers and Antibiotics for Wound Healing Applications.
The aim of our research activity was to obtain a biocompatible nanostructured composite based on naturally derived biopolymers (chitin and sodium alginate) loaded with commercial antibiotics (either Cefuroxime or Cefepime) with dual functions, namely promoting wound healing and assuring the local delivery of the loaded antibiotic. Compositional, structural, and morphological evaluations were performed by using the thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and fourier transform infrared spectroscopy (FTIR) analytical techniques. In order to quantitatively and qualitatively evaluate the biocompatibility of the obtained composites, we performed the tetrazolium-salt (MTT) and agar diffusion in vitro assays on the L929 cell line. The evaluation of antimicrobial potential was evaluated by the viable cell count assay on strains belonging to two clinically relevant bacterial species (i.e., Escherichia coli and Staphylococcus aureus).
Department of Inorganic Chemistry, Physical Chemistry and Electrochemistry, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1-7 Polizu Street, 011061 Bucharest, Romania. radulescu_marius@yahoo.com.
Marius
M
Rădulescu
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1-7 Polizu Street, 011061 Bucharest, Romania. alina_m_h@yahoo.com.|Microbiology Immunology Department, Faculty of Biology, University of Bucharest, 1-3 Portocalelor Lane, Sector 5, 77206 Bucharest, Romania. alina_m_h@yahoo.com.|Research Institute of the University of Bucharest, Life, Environmental and Earth Sciences, Spl. Independentei 91-95, 0500088 Bucharest, Romania. alina_m_h@yahoo.com.
Alina Maria
AM
Holban
Research Center for Microscopic Morphology and Immunology, University of Medicine and Pharmacy of Craiova, PetruRares Street, No. 2, 200349 Craiova, Romania. editor@rjme.ro.
Laurențiu
L
Mogoantă
Research Center for Clinical and Experimental Medicine, University of Medicine and Pharmacy of Craiova 2 PetruRare&#351; Street, 200349 Craiova, Romania. adibalseanu@yahoo.com.
Tudor-Adrian
TA
Bălşeanu
Department of Pharmacognosy &amp; Phytotherapy, Faculty of Pharmacy, University of Medicine and Pharmacy of Craiova, PetruRares Street, No. 2, 200349 Craiova, Romania. mogosanu2006@yahoo.com.
George Dan
GD
Mogoșanu
Department of Life and Environmental Physics, "HoriaHulubei" National Institute of Physics and Nuclear Engineering, Magurele, 077125 Bucharest, Romania. dsavu@nipne.ro.
Diana
D
Savu
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1-7 Polizu Street, 011061 Bucharest, Romania. roxpopescu@yahoo.co.uk.|Department of Life and Environmental Physics, "HoriaHulubei" National Institute of Physics and Nuclear Engineering, Magurele, 077125 Bucharest, Romania. roxpopescu@yahoo.co.uk.
Roxana Cristina
RC
Popescu
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1-7 Polizu Street, 011061 Bucharest, Romania. oana.fufa@gmail.com.|Lasers Department, National Institute for Laser, Plasma and Radiation Physics, Magurele, 077125 Bucharest, Romania. oana.fufa@gmail.com.
Oana
O
Fufă
Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, 1-7 Polizu Street, 011061 Bucharest, Romania. grumezescu@yahoo.com.
Alexandru Mihai
AM
Grumezescu
Laboratory of Microbiology, Biotechnology and Hygiene, Department of Food Science and Technology, Faculty of Agricultural Development, Democritus University of Thrace, 68200 Orestiada, Greece. empezirt@yahoo.gr.
Eugenia
E
Bezirtzoglou
Microbiology Immunology Department, Faculty of Biology, University of Bucharest, 1-3 Portocalelor Lane, Sector 5, 77206 Bucharest, Romania. veronica.Lazar2009@gmail.com.
Veronica
V
Lazar
Microbiology Immunology Department, Faculty of Biology, University of Bucharest, 1-3 Portocalelor Lane, Sector 5, 77206 Bucharest, Romania. carmen_balotescu@yahoo.com.|Research Institute of the University of Bucharest, Life, Environmental and Earth Sciences, Spl. Independentei 91-95, 0500088 Bucharest, Romania. carmen_balotescu@yahoo.com.
Mariana Carmen
MC
Chifiriuc
D000464:Alginates / Q000138:chemical synthesis / Q000737:chemistry* / Q000627:therapeutic use; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000627:therapeutic use; D002686:Chitin / Q000138:chemical synthesis / Q000737:chemistry* / Q000627:therapeutic use; D004926:Escherichia coli / Q000187:drug effects / Q000472:pathogenicity; D020723:Glucuronic Acid / Q000138:chemical synthesis / Q000737:chemistry / Q000627:therapeutic use; D006603:Hexuronic Acids / Q000138:chemical synthesis / Q000737:chemistry / Q000627:therapeutic use; D006801:Humans; D053761:Nanocomposites / Q000737:chemistry / Q000627:therapeutic use*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry / Q000627:therapeutic use; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects / Q000472:pathogenicity; D014945:Wound Healing / Q000187:drug effects*
D000464:Alginates; D000900:Anti-Bacterial Agents; D006603:Hexuronic Acids; D011108:Polymers; D002686:Chitin; D020723:Glucuronic Acid
L929 cell line; anti-biofilm; cefepime; cefuroxime; chitin; drug delivery; rats; sodium alginate