MicroRNA-132 targets PEA-15 and suppresses the progression of astrocytoma in vitro.
Gliomas are highly malignant tumors, the most common of which are astrocytomas. A growing number of studies suggest that dysregulation of miRNAs is a frequent event contributing to the pathogenesis of gliomas. In this study, we found that over-expression of miR-132 inhibited cell proliferation and migration and triggered apoptosis, while knockdown of miR-132 showed opposite effects. PEA-15 was identified as a direct target of miR-132. Reintroduction of PEA-15 without 3'UTR region reversed the inhibitory effects of miR-132 on cell proliferation, migration, and apoptosis. MiR-132 was inversely correlated with the PEA-15 expression. CREB (cAMP response element binding protein) and KLF (Krüppel-like factor 8) were conformed as transcription factors of miR-132, which bidirectionally regulate the expression of miR-132. Our study suggests that miR-132 is an important tumor suppressor of astrocytoma progression by targeting PEA-15, while CREB and KLF can modulate the expression of miR-132, thus providing new insight into the molecular mechanisms underlying astrocytoma progression in vitro.
Department of Neurobiology, Southern Medical University, Guangzhou, 510515, Guangdong, People's Republic of China.|Guangdong Province Key Laboratory of Neuroplasticity of Guangdong Higher Education Institutes, Guangzhou, 510515, Guangdong, People's Republic of China.
Fei
F
Geng
Department of Neurobiology, Southern Medical University, Guangzhou, 510515, Guangdong, People's Republic of China.|Guangdong Province Key Laboratory of Neuroplasticity of Guangdong Higher Education Institutes, Guangzhou, 510515, Guangdong, People's Republic of China.
Jian-Lin
JL
Wu
Department of Pathology, Southern Medical University, Guangzhou, 510515, Guangdong, People's Republic of China.
Gui-Feng
GF
Lu
Department of Neurobiology, Southern Medical University, Guangzhou, 510515, Guangdong, People's Republic of China.|Guangdong Province Key Laboratory of Neuroplasticity of Guangdong Higher Education Institutes, Guangzhou, 510515, Guangdong, People's Republic of China.
Zhi-Ping
ZP
Liang
Department of Neurobiology, Southern Medical University, Guangzhou, 510515, Guangdong, People's Republic of China.|Guangdong Province Key Laboratory of Neuroplasticity of Guangdong Higher Education Institutes, Guangzhou, 510515, Guangdong, People's Republic of China.
Zhuo-Li
ZL
Duan
Department of Neurobiology, Southern Medical University, Guangzhou, 510515, Guangdong, People's Republic of China. guxi124@126.com.|Guangdong Province Key Laboratory of Neuroplasticity of Guangdong Higher Education Institutes, Guangzhou, 510515, Guangdong, People's Republic of China. guxi124@126.com.
Xi
X
Gu
D000704:Analysis of Variance; D017209:Apoptosis / Q000235:genetics / Q000502:physiology; D051017:Apoptosis Regulatory Proteins; D001254:Astrocytoma / Q000378:metabolism* / Q000473:pathology; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D050882:CREB-Binding Protein / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics / Q000502:physiology; D049109:Cell Proliferation / Q000235:genetics / Q000502:physiology; D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics / Q000502:physiology*; D005910:Glioma; D057809:HEK293 Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D051741:Kruppel-Like Transcription Factors; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D012333:RNA, Messenger; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D012844:Sincalide / Q000378:metabolism; D013997:Time Factors; D014162:Transfection
D051017:Apoptosis Regulatory Proteins; D047908:Intracellular Signaling Peptides and Proteins; C507402:KLF8 protein, human; D051741:Kruppel-Like Transcription Factors; C547077:MIRN132 microRNA, human; D035683:MicroRNAs; C092039:PEA15 protein, human; D010750:Phosphoproteins; D012333:RNA, Messenger; D012097:Repressor Proteins; D050882:CREB-Binding Protein; D012844:Sincalide
Astrocytoma; CREB; Glioma; KLF; MiR-132; PEA-15