Analyses of Genetic Variations of Glutathione S-Transferase Mu1 and Theta1 Genes in Bangladeshi Tannery Workers and Healthy Controls.
Glutathione S-transferases (GSTs) belong to a group of multigene detoxification enzymes, which defend cells against oxidative stress. Tannery workers are at risk of oxidative damage that is usually detoxified by GSTs. This study investigated the genotypic frequencies of GST Mu1 (GSTM1) and GST Theta1 (GSTT1) in Bangladeshi tannery workers and healthy controls followed by their status of oxidative stress and total GST activity. Of the 188 individuals, 50.0% had both GSTM1 and GSTT1 (+/+), 12.2% had GSTM1 (+/-), 31.4% had GSTT1 (-/+) alleles, and 6.4% had null genotypes (-/-) with respect to both GSTM1 and GSTT1 alleles. Among 109 healthy controls, 54.1% were double positive, 9.2% had GSTM1 allele, 32.1% had GSTT1 allele, and 4.6% had null genotypes. Out of 79 tannery workers, 44.3% were +/+, 16.8% were +/-, 30.5% were -/+, and 8.4% were -/-. Though the polymorphic genotypes or allelic variants of GSTM1 and GSTT1 were distributed among the study subjects with different frequencies, the differences between the study groups were not statistically significant. GST activity did not vary significantly between the two groups and also among different genotypes while level of lipid peroxidation was significantly higher in tannery workers compared to controls irrespective of their GST genotypes.
Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka 1000, Bangladesh.
Jobaida
J
Akther
Laboratory of Applied Biochemistry, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Akio
A
Ebihara
Laboratory of Applied Biochemistry, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Tsutomu
T
Nakagawa
Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka 1000, Bangladesh.
Laila N
LN
Islam
Laboratory of Applied Biochemistry, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Fumiaki
F
Suzuki
Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka 1000, Bangladesh.
Md Ismail
MI
Hosen
Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka 1000, Bangladesh.
Mahmud
M
Hossain
Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka 1000, Bangladesh.
A H M Nurun
AH
Nabi
D000328:Adult; D000483:Alleles; D000886:Anthropometry; D001459:Bangladesh; D016022:Case-Control Studies; D004587:Electrophoresis, Agar Gel; D005787:Gene Frequency / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D011110:Polymorphism, Genetic; D013633:Tanning; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D005982:Glutathione Transferase; D017392:Thiobarbituric Acid Reactive Substances; C413545:glutathione S-transferase T1