A Novel Approach to Prove Bacterial Leakage of Implant-Abutment Connections In Vitro.
Bacterial leakage from the implant-abutment-interface (IAI) is suspected of contributing to the development of peri-implantitis. The aim of the study was to develop a straightforward test setup to evaluate the bacterial leakage of the IAI of 2-piece implant systems under laboratory conditions. A test suspension of Enterococcus faecium was injected into 7 implants (PerioType Rapid Implants) prior to abutment fixation. The IAI was covered by kanamycin aesculn azide agar (KAAA), which serves as an optical indicator for E. faecium . The specimens were cyclically loaded with a force of 120 N for up to 1 000 000 cycles in a universal testing machine in accordance with the ISO 14801:2007 standard. The color change of the KAAA was recorded. Three of the 7 implants showed bacterial leakage before the cyclic loading test started. The bacterial tightness of the IAIs of the 4 remaining implants lasted for 35 680 ± 22 467 cycles on average. The experimental setup at hand provides the means for a straightforward evaluation of the bacterial tightness of the IAI of 2-piece dental implants.
1   Dental Materials and Biomaterial Research, Department of Prosthodontics, Charité-University Medicine Berlin, Berlin, Germany.
Andreas
A
Wachtel
1   Dental Materials and Biomaterial Research, Department of Prosthodontics, Charité-University Medicine Berlin, Berlin, Germany.
Tycho
T
Zimmermann
1   Dental Materials and Biomaterial Research, Department of Prosthodontics, Charité-University Medicine Berlin, Berlin, Germany.
Tobias
T
Spintig
2   Department of Prosthodontics, Charité-University Medicine Berlin, Berlin, Germany.
Florian
F
Beuer
1   Dental Materials and Biomaterial Research, Department of Prosthodontics, Charité-University Medicine Berlin, Berlin, Germany.
Wolf-Dieter
WD
Müller
1   Dental Materials and Biomaterial Research, Department of Prosthodontics, Charité-University Medicine Berlin, Berlin, Germany.
Andreas Dominik
AD
Schwitalla
D001419:Bacteria / Q000302:isolation & purification*; D000044:Dental Abutments; D015921:Dental Implants; D013293:Enterococcus faecalis / Q000302:isolation & purification; D006801:Humans; D066298:In Vitro Techniques
D015921:Dental Implants
Enterococcus faecium; bacterial leakage; chewing simulation; dental implant-abutment interface; dental implants; peri-implantitis