An iron-oxygen intermediate formed during the catalytic cycle of cysteine dioxygenase.
Cysteine dioxygenase is a key enzyme in the breakdown of cysteine, but its mechanism remains controversial. A combination of spectroscopic and computational studies provides the first evidence of a short-lived intermediate in the catalytic cycle. The intermediate decays within 20 ms and has absorption maxima at 500 and 640 nm.
Department of Chemistry & MacDiarmid Institute for Advanced Materials and Nanotechnology University of Otago, PO Box 56, Dunedin 9054, New Zealand. guy.jameson@otago.ac.nz.
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Tchesnokov
Manchester Institute of Biotechnology and School of Chemical Engineering and Analytical Science, The University of Manchester, 131 Princess Street, Manchester M1 7DN, UK. sam.devisser@manchester.ac.uk.
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Faponle
Department of Chemistry & MacDiarmid Institute for Advanced Materials and Nanotechnology University of Otago, PO Box 56, Dunedin 9054, New Zealand. guy.jameson@otago.ac.nz.
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Davies
Manchester Institute of Biotechnology and School of Chemical Engineering and Analytical Science, The University of Manchester, 131 Princess Street, Manchester M1 7DN, UK. sam.devisser@manchester.ac.uk.
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Quesne
Centre for Free Radical Research, University of Otago, 2 Riccarton Ave, PO Box 4345, Christchurch, New Zealand.
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Turner
Department of Chemistry & MacDiarmid Institute for Advanced Materials and Nanotechnology University of Otago, PO Box 56, Dunedin 9054, New Zealand. guy.jameson@otago.ac.nz.
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Fellner
Department of Chemistry & MacDiarmid Institute for Advanced Materials and Nanotechnology University of Otago, PO Box 56, Dunedin 9054, New Zealand. guy.jameson@otago.ac.nz.
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Souness
Department of Biochemistry, University of Otago, PO Box 56, Dunedin 9054, New Zealand.
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Wilbanks
Manchester Institute of Biotechnology and School of Chemical Engineering and Analytical Science, The University of Manchester, 131 Princess Street, Manchester M1 7DN, UK. sam.devisser@manchester.ac.uk.
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de Visser
Department of Chemistry & MacDiarmid Institute for Advanced Materials and Nanotechnology University of Otago, PO Box 56, Dunedin 9054, New Zealand. guy.jameson@otago.ac.nz.
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Jameson
D055162:Biocatalysis; D050558:Cysteine Dioxygenase / Q000378:metabolism*; D007501:Iron / Q000737:chemistry / Q000378:metabolism*; D008968:Molecular Conformation; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism*
D007501:Iron; D050558:Cysteine Dioxygenase; D010100:Oxygen