Immuno-physiological alterations from AFB1 in rats counteracted by treatments with Lactobacillus paracasei BEJ01 and montmorillonite clay mixture.
High contamination by aflatoxin B1 (AFB1) has been detected in Beja province (Tunisia) in many dairy products and animal feed, which has resulted in many tons of cereals and cereals being removed from the market, causing economic loss. While removal represents a means of reducing risk, exposures still occur. Studies have increasingly focused on means of AFB1 biodegradation/elimination using lactic acid bacteria and clay mineral. In the study here, Lactobacillus paracasei BEJ01 (LP) and montmorilonite clay (MT) were used to reduce the physio-/immunotoxicologic disorders that could develop in rats that underwent AFB1 exposures for a total of 7 consecutive days. The results indicated that rats treated with AFB1 (80 μg/kg BW) alone had significant decreases in lymphocytes in their blood (including B-lymphocytes, CD3(+), CD4(+), and CD8(+) T-lymphocyte subtypes, and NK cells), immunoglobulins (IgA and IgG) and pro-inflammatory cytokines; these rats also had altered oxidative stress status. In contrast, in rats treated with LP + MT (2 × 10(9) cfu/ml [∼ 2 mg/kg] + 0.5 mg MT/kg BW) for a total of 7 days before, concurrent with or after AFB1 treatment, there was a significant blockade/mitigation of each AFB1-impacted parameter. Moreover, treatment with the mixture at any point in relation to AFB1 treatment expectedly caused enhanced TNFα and IL-1β expression relative to control values; all other parameters were comparable to values noted in control rats. Alone, the mixture had no impact on host parameters. From the results here it may be concluded the the LP + MT mixture was effective in protecting these hosts against AFB1-induced immunologic/physiologic disorders and that LP + MT could prevent and/or mitigate AFB1 toxicities in vivo.
a Unit of Immunology, Environmental Microbiology and Cancerology, Faculty of Sciences , University of Carthage , Tunis , Tunisia ;|b Higher Institute of Biotechnology of Monastir, University of Monastir , Monastir , Tunisia ;
Jalila
J
Ben Salah-Abbès
a Unit of Immunology, Environmental Microbiology and Cancerology, Faculty of Sciences , University of Carthage , Tunis , Tunisia ;
Rania
R
Jebali
c National Institute of Genetic Engineering and Biotechnology (NIGEB) , Tehran , Iran.
Hakimeh
H
Sharafi
c National Institute of Genetic Engineering and Biotechnology (NIGEB) , Tehran , Iran.
Kambiz
K
Akbari Noghabi
a Unit of Immunology, Environmental Microbiology and Cancerology, Faculty of Sciences , University of Carthage , Tunis , Tunisia ;
Ridha
R
Oueslati
a Unit of Immunology, Environmental Microbiology and Cancerology, Faculty of Sciences , University of Carthage , Tunis , Tunisia ;
Samir
S
Abbès
D016604:Aflatoxin B1 / Q000378:metabolism* / Q000633:toxicity; D000538:Aluminum Silicates / Q000008:administration & dosage; D000818:Animals; D000917:Antibody Formation; D001546:Bentonite / Q000008:administration & dosage; D001673:Biodegradation, Environmental; D000077215:Clay; D005506:Food Contamination / Q000517:prevention & control*; D005517:Foodborne Diseases / Q000276:immunology / Q000517:prevention & control*; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism; D000069980:Lacticaseibacillus paracasei / Q000276:immunology*; D008231:Lymphopenia; D008297:Male; D018384:Oxidative Stress; D051381:Rats; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014416:Tunisia
D000538:Aluminum Silicates; D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D001546:Bentonite; D016604:Aflatoxin B1; D000077215:Clay
Aflatoxin B1; Lactobacillus; montmorillonite clay; prevention; toxicity