Allosteric modulation of the binding affinity between PQBP1 and the spliceosomal protein U5-15kD.
Polyglutamine tract-binding protein 1 (PQBP1) is an intrinsically disordered protein composed of a small folded WW domain and a long disordered region. PQBP1 binds to spliceosomal proteins WBP11 and U5-15kD through its N-terminal WW domain and C-terminal region, respectively. Here, we reveal that the binding between PQBP1 and WBP11 reduces the binding affinity between PQBP1 and U5-15kD. Our results suggest that the interaction between PQBP1 and WBP11 negatively modulates the U5-15kD binding of PQBP1 by an allosteric mechanism.
Faculty of Pharmaceutical Sciences, University of Toyama, Japan.|Graduate School of Innovative Life Science, University of Toyama, Japan.
Mineyuki
M
Mizuguchi
Faculty of Pharmaceutical Sciences, University of Toyama, Japan.
Takayuki
T
Obita
Faculty of Pharmaceutical Sciences, University of Toyama, Japan.
Asagi
A
Kajiyama
Faculty of Pharmaceutical Sciences, University of Toyama, Japan.
Yuki
Y
Kozakai
Faculty of Pharmaceutical Sciences, University of Toyama, Japan.
Tsuyoshi
T
Nakai
Faculty of Pharmaceutical Sciences, University of Toyama, Japan.
Yuko
Y
Nabeshima
Department of Neuropathology, Medical Research Institute, Tokyo Medical and Dental University, Bunkyo-ku, Japan.|Center for Brain Integration Research, Tokyo Medical and Dental University, Bunkyo-ku, Japan.
Hitoshi
H
Okazawa
D000494:Allosteric Regulation / Q000502:physiology; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006801:Humans; D009687:Nuclear Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding / Q000502:physiology; D000072417:Protein Domains; D000072260:RNA Splicing Factors; D017415:Ribonucleoprotein, U5 Small Nuclear / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C119246:PQBP1 protein, human; D000072260:RNA Splicing Factors; D017415:Ribonucleoprotein, U5 Small Nuclear; C506012:TXNL4A protein, human; C434066:WBP11 protein, human
allosteric mechanism; interaction; intrinsically disordered protein