High detection rate of dog circovirus in diarrheal dogs.
BACKGROUND
Diarrhea is one of the most common clinical symptoms reported in companion animal clinics. Dog circovirus (DogCV) is a new mammalian circovirus that is considered to be a cause of alimentary syndromes such as diarrhea, vomiting and hemorrhagic enteritis. DogCV has previously only been identified in the United States, Italy, Germany (GeneBank accession number: KF887949) and China (GeneBank accession number: KT946839). Therefore, the aims of this study were to determine the prevalence of DogCV in Taiwan and to explore the correlation between diarrhea and DogCV infection. Clinical specimens were collected between 2012 and 2014 from 207 dogs suffering from diarrhea and 160 healthy dogs.


RESULTS
In this study, we developed a sensitive and specific SYBR Green-based real-time PCR assays to detected DogCV in naturally infected animals. Of the analyzed fecal samples from diarrheal dogs and health dogs, 58 (28.0 %) and 19 (11.9 %), respectively, were DogCV positive. The difference in DogCV prevalence was highly significant (P = 0.0002755) in diarrheal dogs.


CONCLUSIONS
This is the first study to reveal that DogCV is currently circulating in domestic dogs in Taiwan and to demonstrate its high detection rate in dogs with diarrhea.
Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Neipu, Pingtung, Taiwan.
Han-Siang
HS
Hsu
Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Neipu, Pingtung, Taiwan.
Ting-Han
TH
Lin
Graduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung-Hsing University, South Dist, Taichung, Taiwan.
Hung-Yi
HY
Wu
Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Neipu, Pingtung, Taiwan.|Veterinary Hospital, College of Veterinary Medicine, National Pingtung University of Science and Technology, Neipu, Pingtung, Taiwan.
Lee-Shuan
LS
Lin
Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Neipu, Pingtung, Taiwan.|Veterinary Hospital, College of Veterinary Medicine, National Pingtung University of Science and Technology, Neipu, Pingtung, Taiwan.
Cheng-Shu
CS
Chung
Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Neipu, Pingtung, Taiwan. mtchiou@mail.npust.edu.tw.|Animal Disease Diagnostic Center, College of Veterinary Medicine, National Pingtung University of Science and Technology, Neipu, Pingtung, Taiwan. mtchiou@mail.npust.edu.tw.
Ming-Tang
MT
Chiou
Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Neipu, Pingtung, Taiwan. cnlin6@mail.npust.edu.tw.|Animal Disease Diagnostic Center, College of Veterinary Medicine, National Pingtung University of Science and Technology, Neipu, Pingtung, Taiwan. cnlin6@mail.npust.edu.tw.
Chao-Nan
http://orcid.org/0000-0003-1911-7535
CN
Lin
D000818:Animals; D018173:Circoviridae Infections / Q000150:complications / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000235:genetics; D003967:Diarrhea / Q000209:etiology / Q000662:veterinary*; D004283:Dog Diseases / Q000453:epidemiology* / Q000821:virology*; D004285:Dogs; D005243:Feces / Q000821:virology; D057805:Pets; D015995:Prevalence; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D013624:Taiwan / Q000453:epidemiology
Diarrhea; Dog circovirus; DogCV; Real-time PCR