Transforming Growth Factor Beta-Induced Factor 2-Linked X (TGIF2LX) Regulates Two Morphogenesis Genes, Nir1 and Nir2 in Human Colorectal.
A member of homeodomain protein namely TGIF2LX has been implicated as a tumor suppressor gene in human malignancy as well as in spermatogenesis. However, to our knowledge, dynamic functional evidence of the TGIF2LX has not yet been provided. The aim of the present study was to investigate the human TGIF2LX target gene(s) using a cDNA-AFLP as a differential display method. A pEGFP-TGIF2LX construct containing the wild-type TGIF2LX cDNA was stably transfected into SW48 cells. UV microscopic analysis and Real-time RT-PCR were used to confirm TGIF2LX expression. The mRNA expressions of TGIF2LX in transfected SW48 cells, the cells containing empty vector (pEGFP-N), and untransfected cells were compared. Also, a Real-time PCR technique was applied to validate cDNA-AFLP results. The results revealed a significant down-regulation and up-regulationby TGIF2LX of Nir1 and Nir2 genes, respectively. The genes are engaged in the cell morphogenesis process. Our findings may provide new insight into the complex molecular pathways underlying colorectal cancer development.
Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Gholam Reza
GR
Mobini
Department of Pharmacology and Toxicology, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Mohammad Hossein
MH
Ghahremani
Cancer Models Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran.
Saeid
S
Amanpour
Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. AND Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Ahmad Reza
AR
Dehpour
Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. AND Colorectal Research Center, Rasoul-e-Akram Hospital, Iran University of Medical Sciences, Tehran, Iran.
Abolfazl
A
Akbari
Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Seyed Mojtaba
SM
Hoseiniharouni
Cancer Models Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran.
Samad
S
Muhammadnejad
Department of Biochemistry and Genetics, School of Medicine, Arak University of Medical Sciences, Arak, Iran.
Maryam
M
Sheikhzade
Farabi Eye Research Center, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Hoda
H
Abedkhojasteh
Farabi Eye Research Center, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Masoumeh
M
Mohebi
Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Manzar
M
Bolhassani
Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran. AND Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Mansour
M
Heidari
D054458:Amplified Fragment Length Polymorphism Analysis; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics*; D015536:Down-Regulation; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D009024:Morphogenesis; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000235:genetics*
D018398:Homeodomain Proteins; D016212:Transforming Growth Factor beta
Colorectal cancer; TGIF2LX; Target genes; Transcription factor; Tumor suppressor; cDNA-AFLP