Expression of AtMed15 of Arabidopsis in yeast causes flocculation and increases ethanol production in yeast culture.
Mediator, a multiprotein complex involved in transcription of class II genes, was first discovered in yeast and then characterized in many metazoans revealing a striking structural conservation of the complex. However, sequences of Mediator subunits are not well conserved raising a question on the functional conservation of these individual subunits. In this study, expression of Med15 of Arabidopsis (AtMed15) in gal11∆ yeast could not complement the function of ScGal11 in galactose metabolism and resistance against cycloheximide. Surprisingly, AtMed15 changed the morphology of the yeast cells. The cells adhered strongly on the surface of the agar media, and showed robust flocculation in the liquid media without affecting the growth. The AtMed15-induced adhesion and flocculation were observed in different carbon sources. Calcium-assisted cell wall-bound mannan-binding proteins were found to be involved in this flocculation, which was unaffected by wide fluctuation of pH or temperatures revealing its constitutive robust nature. Expression of few flocculation related Flo genes was up-regulated in these cells. Interestingly, there was significant increase in ethanol production by the yeast expressing AtMed15. Robust and constitutive flocculation and increased ethanol production by yeast cells harbouring AtMed15 indicate an opportunity of its important usage in biotechnology industries.
National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India.
Pradeep
P
Dahiya
National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India.
Divya S
DS
Bhat
National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India.
Jitendra K
JK
Thakur
D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002448:Cell Adhesion; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005427:Flocculation; D015870:Gene Expression; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*
D029681:Arabidopsis Proteins; D011994:Recombinant Proteins; D000431:Ethanol