Near Infrared Dye Conjugated Nanogels for Combined Photodynamic and Photothermal Therapies.
There is a need for new and smart formulations that will help overcome the limitations of organic dyes used in photodynamic (PDT) and photothermal (PTT) therapy and significantly accelerate their clinical translation. Therefore the aim of this work was to create a responsive nanogel scaffold as a smart vehicle for dye administration. We developed a methodology that enables the conjugation of organic dyes to thermoresponsive nanogels and yields biocompatible, nanometer-sized products with low polydispersity. The potential of the dye-nanogel conjugate as a photothermal and photodynamic agent has been demonstrated by an in vitro evaluation with a model human carcinoma cell line. Additionally, confocal cell images showed their cellular uptake profile and their potential for bioimaging and intracellular drug delivery. These conjugates are a promising scaffold as a theranostic agents and will enable further applications in combination with controlled drug release.
Institut für Chemie und Biochemie, Freie Universität Berlin, Takustrasse 3, 14195, Berlin, Germany.
Mazdak
M
Asadian-Birjand
Institut für Chemie und Biochemie, Freie Universität Berlin, Takustrasse 3, 14195, Berlin, Germany.
Julian
J
Bergueiro
Institut für Chemie und Biochemie, Freie Universität Berlin, Takustrasse 3, 14195, Berlin, Germany.
Stefanie
S
Wedepohl
Institut für Chemie und Biochemie, Freie Universität Berlin, Takustrasse 3, 14195, Berlin, Germany. marcelo.calderon@fu-berlin.de.
Marcelo
M
Calderón
D045744:Cell Line, Tumor; D004396:Coloring Agents / Q000737:chemistry / Q000494:pharmacology; D016503:Drug Delivery Systems / Q000379:methods*; D005260:Female; D005782:Gels; D006801:Humans; D006979:Hyperthermia, Induced / Q000379:methods*; D007259:Infrared Rays; D053758:Nanoparticles / Q000737:chemistry / Q000627:therapeutic use; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D010778:Photochemotherapy / Q000379:methods*
D004396:Coloring Agents; D005782:Gels
cell ablation; nanogel; near infrared light; photodynamic therapy; photothermal therapy