Detection of Variation in Long-Term Micropropagated Mature Pistachio via DNA-Based Molecular Markers.
Determination of genetic stability of in vitro-grown plantlets is needed for safe and large-scale production of mature trees. In this study, genetic variation of long-term micropropagated mature pistachio developed through direct shoot bud regeneration using apical buds (protocol A) and in vitro-derived leaves (protocol B) was assessed via DNA-based molecular markers. Randomly amplified polymorphic DNA (RAPD), inter-simple sequence repeat (ISSR), and amplified fragment length polymorphism (AFLP) were employed, and the obtained PIC values from RAPD (0.226), ISSR (0.220), and AFLP (0.241) showed that micropropagation of pistachio for different periods of time resulted in "reasonable polymorphism" among donor plant and its 18 clones. Mantel's test showed a consistence polymorphism level between marker systems based on similarity matrices. In conclusion, this is the first study on occurrence of genetic variability in long-term micropropagated mature pistachio plantlets. The obtained results clearly indicated that different marker approaches used in this study are reliable for assessing tissue culture-induced variations in long-term cultured pistachio plantlets.
Faculty of Basic Sciences, Department of Molecular Biology and Genetics, Gebze Technical University, 41400, Kocaeli, Turkey.
Hülya
H
Akdemir
Vocational School of Health Services, Department of Medical Services and Techniques, Bingöl University, 12000, Bingöl, Turkey.
Veysel
V
Suzerer
Faculty of Science and Art, Department of Biology, Batman University, 72100, Batman, Turkey.
Engin
E
Tilkat
Faculty of Science, Department of Biology, Dicle University, 21280, Diyarbakır, Turkey.
Ahmet
A
Onay
Faculty of Basic Sciences, Department of Molecular Biology and Genetics, Gebze Technical University, 41400, Kocaeli, Turkey. yelda75@yahoo.com.|Institute of Biotechnology, Gebze Technical University, 41400, Kocaeli, Turkey. yelda75@yahoo.com.
Yelda Ozden
YO
Çiftçi
D054458:Amplified Fragment Length Polymorphism Analysis; D016000:Cluster Analysis; D018744:DNA, Plant / Q000235:genetics*; D005819:Genetic Markers; D014644:Genetic Variation; D018895:Microsatellite Repeats / Q000235:genetics; D010802:Phylogeny; D027927:Pistacia / Q000235:genetics* / Q000254:growth & development*; D025341:Principal Component Analysis; D019105:Random Amplified Polymorphic DNA Technique; D013997:Time Factors
D018744:DNA, Plant; D005819:Genetic Markers
AFLP; ISSR; Pistacia vera; RAPD; Tissue culture-induced variation