Survey on association between Mycoplasma hominis endocervical infection and spontaneous abortion using Polymerase Chain Reaction.
BACKGROUND
Mycoplasma infections are suggested as etiology of adverse pregnancy outcomes.


OBJECTIVE
The aim of this study was to evaluate the association of Mycoplasma hominis (M. hominis) infection and spontaneous abortion among pregnant women.


METHODS
In this case-control study that was conducted from August 2012 to January 2013, totally, 109 women were included with spontaneous abortion with gestational ages of 10-20 weeks (Cases), and 109 women with normal pregnancy with gestational ages between 20-37 weeks (Controls) in Sanandaj, Iran. Using specific primers and extracted DNA from endocervical swabs, a PCR test was conducted for detection of M. hominis infection in women. For comparison of qualitative and quantitative variables, independent Fisher tests were used and p<0.05 was considered significant.


RESULTS
The total frequency of M. hominis infection was 6 (2.75%) in women. The frequency of M. hominis infection was 2 (1.83%) in the case group (spontaneous abortion) and 4 (3.66%) in the control group, respectively. In both case and control groups, no association was seen between M.hominis infection and spontaneous abortion (OR=0. 49, CI 95%: 0.08-2.73, p=0. 683).


CONCLUSIONS
M. hominis was positive in the genital tract of some pregnant women, but it was not associated with spontaneous abortion. However, to prevent adverse pregnancy outcomes in women, foetus and neonate, routine screening and treatment for the genital Mycoplasma is recommended.
Social Determinants of Health Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.; Department of Genecology, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Fariba
F
Farhadifar
Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.; Microbiology Department, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Mazaher
M
Khodabandehloo
Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.; Microbiology Department, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Rashid
R
Ramazanzadeh
Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.; Microbiology Department, Kurdistan University of Medical Sciences, Sanandaj, Iran.; Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Samaneh
S
Rouhi
Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.; Microbiology Department, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Amjad
A
Ahmadi
Social Determinants of Health Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Ebrahim
E
Ghaderi
Social Determinants of Health Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Daem
D
Roshani
Department of Genecology, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Nasrin
N
Soofizadeh
Department of Genecology, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Masoomeh
M
Rezzaii
Miscarriage; Mycoplasma hominis; Spontaneous Abortion; Women.