Lack of Platelet-Activating Factor Receptor Attenuates Experimental Food Allergy but Not Its Metabolic Alterations regarding Adipokine Levels.
Platelet-activating factor (PAF) is known to be an important mediator of anaphylaxis. However, there is a lack of information in the literature about the role of PAF in food allergy. The aim of this work was to elucidate the participation of PAF during food allergy development and the consequent adipose tissue inflammation along with its alterations. Our data demonstrated that, both before oral challenge and after 7 days receiving ovalbumin (OVA) diet, OVA-sensitized mice lacking the PAF receptor (PAFR) showed a decreased level of anti-OVA IgE associated with attenuated allergic markers in comparison to wild type (WT) mice. Moreover, there was less body weight and adipose tissue loss in PAFR-deficient mice. However, some features of inflamed adipose tissue presented by sensitized PAFR-deficient and WT mice after oral challenge were similar, such as a higher rate of rolling leukocytes in this tissue and lower circulating levels of adipokines (resistin and adiponectin) in comparison to nonsensitized mice. Therefore, PAF signaling through PAFR is important for the allergic response to OVA but not for the adipokine alterations caused by this inflammatory process. Our work clarifies some effects of PAF during food allergy along with its role on the metabolic consequences of this inflammatory process.
Department of Biochemistry and Immunology, Biological Sciences Institute, Federal University of Minas Gerais (UFMG), 31270-901 Belo Horizonte, MG, Brazil.
Nathália Vieira
NV
Batista
Departamento de Morfologia, ICB, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, Pampulha, 31270-901 Belo Horizonte, MG, Brazil.
Roberta Cristelli
RC
Fonseca
Departamento de Morfologia, ICB, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, Pampulha, 31270-901 Belo Horizonte, MG, Brazil.
Denise
D
Perez
Departamento de Morfologia, ICB, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, Pampulha, 31270-901 Belo Horizonte, MG, Brazil.
Rafaela Vaz Sousa
RV
Pereira
Department of Biochemistry and Immunology, Biological Sciences Institute, Federal University of Minas Gerais (UFMG), 31270-901 Belo Horizonte, MG, Brazil.
Juliana
J
de Lima Alves
Departamento de Morfologia, ICB, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, Pampulha, 31270-901 Belo Horizonte, MG, Brazil.
Vanessa
V
Pinho
Department of Biochemistry and Immunology, Biological Sciences Institute, Federal University of Minas Gerais (UFMG), 31270-901 Belo Horizonte, MG, Brazil.
Ana Maria Caetano
AM
Faria
Departamento de Morfologia, ICB, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, Pampulha, 31270-901 Belo Horizonte, MG, Brazil.
Denise Carmona
DC
Cara
D054392:Adipokines / Q000097:blood*; D000273:Adipose Tissue / Q000276:immunology / Q000378:metabolism; D000821:Animal Feed; D000818:Animals; D015415:Biomarkers / Q000097:blood; D001835:Body Weight / Q000276:immunology; D004032:Diet; D005512:Food Hypersensitivity / Q000097:blood / Q000276:immunology* / Q000378:metabolism*; D007073:Immunoglobulin E / Q000276:immunology; D007249:Inflammation / Q000097:blood / Q000276:immunology / Q000378:metabolism; D007962:Leukocytes / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D023421:Models, Animal; D010047:Ovalbumin / Q000276:immunology; D010980:Platelet Membrane Glycoproteins / Q000276:immunology* / Q000378:metabolism*; D043562:Receptors, G-Protein-Coupled / Q000276:immunology* / Q000378:metabolism*
D054392:Adipokines; D015415:Biomarkers; D010980:Platelet Membrane Glycoproteins; D043562:Receptors, G-Protein-Coupled; C039351:platelet activating factor receptor; D007073:Immunoglobulin E; D010047:Ovalbumin