Rosmarinic Acid Attenuates Airway Inflammation and Hyperresponsiveness in a Murine Model of Asthma.
Rosmarinic acid (RA) has numerous pharmacologic effects, including anti-oxidant, anti-inflammatory, and analgesic effects. This study aimed to evaluate the preventive activity of RA in a murine model of asthma and to investigate its possible molecular mechanisms. Female BALB/c mice sensitized and challenged with ovalbumin (Ova) were pretreated with RA (5, 10 or 20 mg/kg) at 1 h before Ova challenge. The results demonstrated that RA markedly inhibited increases in inflammatory cells and Th2 cytokines in the bronchoalveolar lavage fluid (BALF), significantly reduced the total IgE and Ova-specific IgE concentrations, and greatly ameliorated airway hyperresponsiveness (AHR) compared with the control Ova-induced mice. Histological analyses showed that RA substantially decreased the number of inflammatory cells and mucus hypersecretion in the airway. In addition, our results suggested that the protective effects of RA might be mediated by the suppression of ERK, JNK and p38 phosphorylation and activation of nuclear factor-κB (NF-κB). Furthermore, RA pretreatment resulted in a noticeable reduction in AMCase, CCL11, CCR3, Ym2 and E-selectin mRNA expression in lung tissues. These findings suggest that RA may effectively delay the progression of airway inflammation.
College of Animal Science and Technology, Guangxi University, Nanning 530005, China. liangzhengmin815@163.com.
Zhengmin
Z
Liang
College of Animal Science and Technology, Guangxi University, Nanning 530005, China. yangfengxu1992@gmail.com.
Yangfeng
Y
Xu
College of Animal Science and Technology, Guangxi University, Nanning 530005, China. xuemeiwen1992@163.com.
Xuemei
X
Wen
College of Animal Science and Technology, Guangxi University, Nanning 530005, China. 15296494675@163.com.
Haiying
H
Nie
College of Animal Science and Technology, Guangxi University, Nanning 530005, China. tingjunhu@126.com.
Tingjun
T
Hu
College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, Jilin, China. xiaofengyang@hotmail.com.
Xiaofeng
X
Yang
College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, Jilin, China. 421591993@163.com.
Xiao
X
Chu
College of Animal Science and Technology, Guangxi University, Nanning 530005, China. yangjian0104@163.com.
Jian
J
Yang
College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, Jilin, China. Dengxm@jlu.edu.cn.
Xuming
X
Deng
College of Animal Science and Technology, Guangxi University, Nanning 530005, China. ahong18@vip.sina.com.
Jiakang
J
He
D000700:Analgesics / Q000008:administration & dosage / Q000737:chemistry; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000737:chemistry; D001249:Asthma / Q000188:drug therapy* / Q000473:pathology; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002934:Cinnamates / Q000008:administration & dosage* / Q000737:chemistry; D053630:Depsides / Q000008:administration & dosage* / Q000737:chemistry; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007249:Inflammation / Q000188:drug therapy* / Q000473:pathology; D008168:Lung / Q000187:drug effects / Q000473:pathology; D051379:Mice; D000097123:Rosmarinic Acid
D000700:Analgesics; D000975:Antioxidants; D002934:Cinnamates; D053630:Depsides; D000097123:Rosmarinic Acid
MAPK; NF-κB; RA; asthma; mRNA expression; preventive