PCR-based detection of Mycobacterium avium subsp. paratuberculosis infection in cattle in South Korea using fecal samples.
Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of bovine paratuberculosis (PTB). The first step in the control of PTB is the identification and isolation of sub-clinical fecal shedders from the herd. In the current study, real-time and nested PCR targeting MAP-specific genetic elements (IS900 and ISMAP02) DNA isolated from fecal samples were used to detect MAP infection in cattle. Of the 1,562 fecal samples obtained from 37 herds, regardless of diarrhea, 35 samples tested positive in both IS900-targeted real-time and ISMAP02-targeted nested PCR. At the herd level, 12 of the 37 herds were found to be positive for MAP. Detection rates of the PCR tests were similar to those reported for ELISA-based methods. These results suggest that PCR can be used to detect sub-clinical fecal shedders of MAP.
Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.
Hong-Tae
HT
Park
Min-Kyoung
MK
Shin
Hyun-Eui
HE
Park
Yong-Il
YI
Cho
Han Sang
HS
Yoo
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D016927:Mycobacterium avium subsp. paratuberculosis; D010283:Paratuberculosis / Q000175:diagnosis* / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D056910:Republic of Korea