Qualitative and Quantitative Determination and Resistance Patterns of Salmonella from Poultry Carcasses.
Poultry products are important in the transmission of zoonotic pathogens, mainly Salmonella. This genus causes millions of foodborne diseases worldwide every year. Cross-contamination by food sources in human cases of salmonellosis and the increase in resistant strains have become important issues. A qualitative and quantitative Salmonella detection method was utilized in a poultry slaughterhouse in São Paulo State, Brazil. We collected 33 samples from different batches of carcasses. Each sample was analyzed at three process points: postbleeding, postdefeathering, and postchilling. A fourth point, retail simulation, was added to simulate retail market storage at 5°C for 72 h. The qualitative methods revealed 100% (33 samples) contamination at postbleeding, 39% (13 samples) contamination at postdefeathering, 58% (19 samples) contamination at postchilling, and 30% (10 samples) contamination at the retail simulation. The quantitative results, determined by the most-probable-number (MPN) technique, ranged from <0.03 to >2,400 MPN/g. We identified 23 Salmonella serovars; the most prevalent were Mbandaka, Senftenberg, and Enteritidis. Resistance to nalidixic acid was significantly more common (P < 0.05) than resistance to other antimicrobial agents. Five multidrug-resistant strains were identified. This study contributes important epidemiological data and demonstrates the need to improve sanitary conditions in slaughterhouses.
Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Science, Botucatu, São Paulo 18.618-970, Brazil. rsyvet@yahoo.com.br.
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Yamatogi
Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Science, Botucatu, São Paulo 18.618-970, Brazil.
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Oliveira
Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Science, Botucatu, São Paulo 18.618-970, Brazil.
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Possebon
Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Science, Botucatu, São Paulo 18.618-970, Brazil.
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Pantoja
Ministry of Agriculture, Livestock, and Food Supply, Botucatu, Sãoo Paulo 18618-970, Brazil.
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Joaquim
Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Science, Botucatu, São Paulo 18.618-970, Brazil.
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Pinto
Department of Microbiology and Immunology, Bioscience Institute, Universidade Estadual Paulista, Botucatu, São Paulo 18.618-970, Brazil.
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Araújo
D000818:Animals; D000900:Anti-Bacterial Agents; D001938:Brazil; D002645:Chickens; D005506:Food Contamination; D006801:Humans; D011200:Poultry; D015995:Prevalence; D012475:Salmonella / Q000145:classification
D000900:Anti-Bacterial Agents