Preparative Separation and Purification of the Total Flavonoids in Scorzonera austriaca with Macroporous Resins.
The use of macroporous resins for the separation and purification of total flavonoids to obtain high-purity total flavonoids from Scorzonera austriaca was studied. The optimal conditions for separation and purification of total flavonoids in S. austriaca with macroporous resins were as follows: D4020 resin columns were loaded with crude flavonoid extract solution, and after reaching adsorptive saturation, the columns were eluted successively with 5 bed volumes (BV) of water, 5 BV of 5% (v/v) aqueous ethanol and 5 BV of 30% (v/v) aqueous ethanol at an elute flow rate of 2 BV·h(-1). Total flavonoids were obtained from the 30% aqueous ethanol eluate by vacuum distillation recovery. The content of flavonoid compounds in the total flavonoids was 93.5%, which represents an improvement by about 150%. In addition, five flavonoid compounds in the product were identified as 2″-O-β-d-xylopyranosyl isoorientin, 6-C-α-l-arabipyranosyl orientin, orientin, isoorientin and vitexin by LC-ESI-MS analysis and internal standard methods. The results in this study could represent a method for the large-scale production of total flavonoids from S. austriaca.
Department of Pharmacognosy, School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China. 13514486691@163.com.
Yang
Y
Xie
Department of Pharmacy, the Second Part of the First Hospital, Jilin University, Changchun 130021, China. guoqiushi126@126.com.
Qiu-Shi
QS
Guo
Department of Pharmacognosy, School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China. wgs@jlu.edu.cn.
Guang-Shu
GS
Wang
D047310:Apigenin / Q000737:chemistry* / Q000302:isolation & purification; D002853:Chromatography, Liquid; D000431:Ethanol / Q000737:chemistry; D005419:Flavonoids / Q000737:chemistry* / Q000302:isolation & purification; D005960:Glucosides / Q000737:chemistry* / Q000302:isolation & purification; D047311:Luteolin / Q000737:chemistry* / Q000302:isolation & purification; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000737:chemistry*; D012116:Resins, Plant / Q000737:chemistry; D031186:Scorzonera / Q000737:chemistry
D005419:Flavonoids; D005960:Glucosides; D010936:Plant Extracts; D012116:Resins, Plant; D000431:Ethanol; D047310:Apigenin; C032731:vitexin; C057912:homoorientin; C065886:orientin; D047311:Luteolin
Scorzonera austriaca; flavonoids; macroporous resins