Electron Transport System in Pseudomonas hydrogenothermophila Strain TH-1, a Thermophilic Hydrogen-oxidizing Bacterium.
The electron transport system in Pseudomonas hydrogenothermophila strain TH-1, a thermophilic, aerobic hydrogen-oxidizing bacterium, was studied using a membrane fraction, an ubiquinone-depleted membrane fraction (Q(-) MF), and an ubiqunone-reconstituted membrane fraction (Q(+) MF). From spectrophotometric and TLC analyses, cytochromes b and c, ubiquinone-8 (Q8), and cytochrome o were detected as respiratory components in the membrane fraction of P. hydrogenothermophila. Spectrophotometric analyses also showed that reduction of all the components in the membrane fraction depends on a membrane-bound hydrogenase reaction. In Q(-) MF, reduction of cytochromes b and c depending on membrane-bound hydrogenase reaction could not be observed. However, the reduction was restored in Q(+) MF. From these results, a possible electron transport system in P. hydrogenothermophila strain TH-1 is proposed.
a Department of Agricultural Chemistry , The University of Tokyo , Bunkyo-ku, Tokyo 113 , Japan.
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Ishii
a Department of Agricultural Chemistry , The University of Tokyo , Bunkyo-ku, Tokyo 113 , Japan.
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Ono
a Department of Agricultural Chemistry , The University of Tokyo , Bunkyo-ku, Tokyo 113 , Japan.|b Riken , Japan Collection of Microorganisms , Wako-shi, Saitama 351-01 , Japan.
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Suzuki
a Department of Agricultural Chemistry , The University of Tokyo , Bunkyo-ku, Tokyo 113 , Japan.
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Igarashi
a Department of Agricultural Chemistry , The University of Tokyo , Bunkyo-ku, Tokyo 113 , Japan.
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Kodama