First genetic linkage map for the mud crab (Scylla paramamosain) constructed using microsatellite and AFLP markers.
The mud crab (Scylla paramamosain) is of economic importance for the fisheries and aquaculture industry in China. In this study, we constructed the first genetic linkage map for this species using microsatellite and amplified fragment length polymorphism (AFLP) markers. The map consisted of 65 linkage groups, including 34 triplets and 9 doublets. A total of 212 molecular markers were mapped, including 60 microsatellites and 152 AFLP markers. The linkage groups ranged from 7 to 102.5 cM and covered 2746.4 cM in length. The mean length was 42.3 cM per linkage group, and the mean spacing was 18.68 cM. The genome size was estimated to be 5539.62 cM, with 50% coverage by the present map. Moreover, we reported 5 transcriptome-derived polymorphic microsatellite markers and characterized their polymorphism in a first-generation family. This study will facilitate studies on high-density maps and molecular marker-assisted selection in S. paramamosain and related crustacean species.
East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China.|Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture, Shanghai, China.
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Ma
East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China.|Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture, Shanghai, China.
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Li
East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China.|Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture, Shanghai, China.
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Feng
East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China.|Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture, Shanghai, China.
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Ma
East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China.|Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture, Shanghai, China.
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Wang
East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China.|Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture, Shanghai, China.
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Chen
East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China.|Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation, Ministry of Agriculture, Shanghai, China.
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D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D003386:Brachyura / Q000235:genetics*; D002681:China; D008040:Genetic Linkage; D018895:Microsatellite Repeats / Q000235:genetics*; D059467:Transcriptome / Q000235:genetics*