Overexpression of Peroxiredoxin 6 Protects Neoplastic Cells against Apoptosis in Canine Haemangiosarcoma.
Canine haemangiosarcoma (HSA), like human angiosarcoma, is an uncommon malignant vascular endothelial cell tumour associated with a poor prognosis. The peroxiredoxin (PRDX) family of peroxidases, which comprises six members in mammals (PRDX1-6), might contribute to cancer cell survival in the face of oxidative stress as these proteins exhibit frequent upregulation in cancer cells. In this study, we investigated the expression levels of PRDX6 in spontaneously arising primary canine HSAs by immunohistochemical analysis, identifying marked expression of this protein. Both PRDX6 mRNA and protein were overexpressed in HSA cell lines compared with normal canine endothelial cells, although some variation was observed between the different HSA cell lines. Small interfering RNA-induced downregulation of PRDX6 promoted apoptosis in the HSA cell lines. The observation that PRDX6 suppression increased the cytotoxicity of these cells suggests that PRDX6 might play an important cytoprotective role.
Laboratory of Veterinary Pathology, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, Japan; Department of Pathology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt.
Sh
Sh
Anwar
Laboratory of Veterinary Pathology, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, Japan.
T
T
Yanai
Laboratory of Veterinary Pathology, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, Japan; Comparative Cancer Centre, Gifu University, 1-1 Yanagido, Gifu, Japan. Electronic address: shiroki@gifu-u.ac.jp.
H
H
Sakai
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D014408:Biomarkers, Tumor / Q000032:analysis*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D005260:Female; D055785:Gene Knockdown Techniques; D006394:Hemangiosarcoma / Q000662:veterinary*; D007150:Immunohistochemistry; D008297:Male; D054465:Peroxiredoxin VI / Q000032:analysis / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D014408:Biomarkers, Tumor; D054465:Peroxiredoxin VI
apoptosis; dog; haemangiosarcoma; peroxiredoxin 6