Biomimetic self-templating optical structures fabricated by genetically engineered M13 bacteriophage.
Here, we describe a highly sensitive and selective surface plasmon resonance sensor system by utilizing self-assembly of genetically engineered M13 bacteriophage. About 2700 copies of genetically expressed peptide copies give superior selectivity and sensitivity to M13 phage-based SPR sensor. Furthermore, the sensitivity of the M13 phage-based SPR sensor was enhanced due to the aligning of receptor matrix in specific direction. Incorporation of specific binding peptide (His Pro Gln: HPQ) gives M13 bacteriophage high selectivity for the streptavidin. Our M13 phage-based SPR sensor takes advantage of simplicity of self-assembly compared with relatively complex photolithography techniques or chemical conjugations. Additionally, designed structure which is composed of functionalized M13 bacteriophage can simultaneously improve the sensitivity and selectivity of SPR sensor evidently. By taking advantages of the genetic engineering and self-assembly, we propose the simple method for fabricating novel M13 phage-based SPR sensor system which has a high sensitivity and high selectivity.
Department of Nano Fusion Technology, Pusan National University, Busan 609-735, South Korea; BK21 PLUS Nanoconvergence Technology Division, Pusan National University (PNU), Busan 46241, Republic of Korea.
Won-Geun
WG
Kim
Department of Cogno-Mechatronics Engineering, Pusan National University, Busan 609-735, South Korea.
Hyerin
H
Song
Department of Nano Fusion Technology, Pusan National University, Busan 609-735, South Korea; BK21 PLUS Nanoconvergence Technology Division, Pusan National University (PNU), Busan 46241, Republic of Korea.
Chuntae
C
Kim
BK21 PLUS Nanoconvergence Technology Division, Pusan National University (PNU), Busan 46241, Republic of Korea.
Jong-Sik
JS
Moon
Department of Cogno-Mechatronics Engineering, Pusan National University, Busan 609-735, South Korea. Electronic address: k.kim@pusan.ac.kr.
Kyujung
K
Kim
Department of Bioengineering, University of California, Berkeley, Biological Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley Nanoscience and Nanoengineering Institute, Berkeley, CA 94720, USA. Electronic address: leesw@berkeley.edu.
Seung-Wuk
SW
Lee
Department of Nano Fusion Technology, Pusan National University, Busan 609-735, South Korea; BK21 PLUS Nanoconvergence Technology Division, Pusan National University (PNU), Busan 46241, Republic of Korea; Department of Bioengineering, University of California, Berkeley, Biological Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley Nanoscience and Nanoengineering Institute, Berkeley, CA 94720, USA; Department of Nanoenergy Engineering, Pusan National University, Busan 609-735, South Korea. Electronic address: ojw@pusan.ac.kr.
Jin-Woo
JW
Oh
D017104:Bacteriophage M13 / Q000235:genetics* / Q000378:metabolism; D032701:Biomimetics / Q000379:methods; D005818:Genetic Engineering / Q000379:methods; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D019151:Peptide Library; D010455:Peptides / Q000235:genetics* / Q000378:metabolism*; D019809:Streptavidin / Q000378:metabolism*; D020349:Surface Plasmon Resonance / Q000379:methods
D019151:Peptide Library; D010455:Peptides; D019809:Streptavidin
Biomimicry; M13 bacteriophage; Self-assembly; Sensor; Surface plasmon resonance