Identification of miRNomes associated with adult neurogenesis after stroke using Argonaute 2-based RNA sequencing.
Neurogenesis is associated with functional recovery after stroke. However, the underlying molecular mechanisms have not been fully investigated. Using an Ago2-based RNA immunoprecipitation to immunoprecipated Ago2-RNA complexes followed by RNA sequencing (Ago2 RIP-seq) approach, we profiled the miRNomes in neural progenitor cells (NPCs) harvested from the subventricular zone (SVZ) of the lateral ventricles of young adult rats. We identified more than 7 and 15 million reads in normal and ischemic NPC libraries, respectively. We found that stroke substantially changed Ago2-associated miRNA profiles in NPCs compared to those in non-ischemic NPCs. We also discovered a new complex repertoire of isomiRs and multiple miRNA-miRNA* pairs and numerous novel miRNAs in the non-ischemic and ischemic NPCs. Among them, pc-3p-17172 significantly regulated NPC proliferation and neuronal differentiation. Collectively, the present study reveals profiles of Ago2-associated miRNomes in non-ischemic and ischemic NPCs, which provide a molecular basis to further investigate the role of miRNAs in mediating adult neurogenesis under physiological and ischemic conditions.
a Department of Neurology , Henry Ford Health System , Detroit , MI , USA.
Xian Shuang
XS
Liu
a Department of Neurology , Henry Ford Health System , Detroit , MI , USA.
Bao Yan
BY
Fan
a Department of Neurology , Henry Ford Health System , Detroit , MI , USA.|b Sichuan Key Laboratory of Medical Imaging and Department of Immunology , North Sichuan Medical University , Nanchong , Sichuan , China.
Wan Long
WL
Pan
a Department of Neurology , Henry Ford Health System , Detroit , MI , USA.
Chao
C
Li
c Department of Public Health Sciences , Henry Ford Health System , Detroit , MI , USA.|d Center for Bioinformatics , Henry Ford Health System , Detroit , MI , USA.
Albert M
AM
Levin
a Department of Neurology , Henry Ford Health System , Detroit , MI , USA.
Xinli
X
Wang
a Department of Neurology , Henry Ford Health System , Detroit , MI , USA.
Rui Lan
RL
Zhang
a Department of Neurology , Henry Ford Health System , Detroit , MI , USA.
Thomas M
TM
Zervos
e Department of Radiology , Wayne State University , Detroit , MI , USA.
Jiani
J
Hu
f Sichuan Key Laboratory of Medical Imaging and Department of Radiology , Affiliated Hospital of North Sichuan Medical University , Nanchong , Sichuan , China.
Xiao Ming
XM
Zhang
a Department of Neurology , Henry Ford Health System , Detroit , MI , USA.|g Department of Physics , Oakland University , Rochester , MI , USA.
Michael
M
Chopp
a Department of Neurology , Henry Ford Health System , Detroit , MI , USA.
Zheng Gang
ZG
Zhang
D000328:Adult; D000704:Analysis of Variance; D000818:Animals; D060565:Argonaute Proteins / Q000235:genetics / Q000378:metabolism*; D049109:Cell Proliferation; D006801:Humans; D020547:Lateral Ventricles / Q000737:chemistry; D008297:Male; D035683:MicroRNAs / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D058953:Neural Stem Cells / Q000378:metabolism* / Q000473:pathology; D055495:Neurogenesis / Q000502:physiology*; D061251:Primary Cell Culture; D051381:Rats; D017208:Rats, Wistar; D017423:Sequence Analysis, RNA; D020521:Stroke / Q000378:metabolism* / Q000473:pathology; D059467:Transcriptome
C582332:Ago2 protein, rat; D060565:Argonaute Proteins; D035683:MicroRNAs
Ago2; RNA sequencing; microRNA; neural progenitor cells; neurogenesis; stroke