In vitro antimicrobial activity of honokiol against Staphylococcus aureus in biofilm mode.
Staphylococcus aureus (S. aureus) can attach to food, host tissues and the surfaces of medical implants and form a biofilm, which makes it difficult to eliminate. The purpose of this study was to evaluate the effect of honokiol on biofilm-grown S. aureus. In this report, honokiol showed effective antibacterial activity against S. aureus in biofilms. S. aureus isolates are capable of producing distinct types of biofilms mediated by polysaccharide intercellular adhesion (PIA) or extracellular DNA (eDNA). The biofilms' susceptibility to honokiol was evaluated using confocal laser scanning microscopy (CLSM) analysis. The transcript levels of the biofilm-related genes, the expression of PIA, and the amount of eDNA of biofilm-grown S. aureus exposed to honokiol were also investigated. The results of this study show that honokiol can detach existing biofilms, kill bacteria in biofilms, and simultaneously inhibit the transcript levels of sarA, cidA and icaA, eDNA release, and the expression of PIA.
a Department of Food Quality and Safety, College of Food Science and Engineering, Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis , Jilin University , Changchun 130062 , China.
Wen-Li
WL
Li
a Department of Food Quality and Safety, College of Food Science and Engineering, Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis , Jilin University , Changchun 130062 , China.
Xing-Chen
XC
Zhao
a Department of Food Quality and Safety, College of Food Science and Engineering, Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis , Jilin University , Changchun 130062 , China.
Zi-Wen
ZW
Zhao
a Department of Food Quality and Safety, College of Food Science and Engineering, Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis , Jilin University , Changchun 130062 , China.
Yan-Jun
YJ
Huang
a Department of Food Quality and Safety, College of Food Science and Engineering, Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis , Jilin University , Changchun 130062 , China.
Xuan-Zhi
XZ
Zhu
b Jilin Enrty-exit Inspection and Quarantine Bureau , Changchun 130062 , China.
Ri-Zeng
RZ
Meng
a Department of Food Quality and Safety, College of Food Science and Engineering, Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis , Jilin University , Changchun 130062 , China.
Ce
C
Shi
a Department of Food Quality and Safety, College of Food Science and Engineering, Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis , Jilin University , Changchun 130062 , China.
Lu
L
Yu
a Department of Food Quality and Safety, College of Food Science and Engineering, Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis , Jilin University , Changchun 130062 , China.
Na
N
Guo
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D001713:Biphenyl Compounds / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*; D004247:DNA / Q000032:analysis; D017705:Lignans / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*; D015394:Molecular Structure; D013211:Staphylococcus aureus / Q000187:drug effects*; D000498:Allyl Compounds; D010636:Phenols
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D001713:Biphenyl Compounds; D004247:DNA; D017705:Lignans; C005499:honokiol; D000498:Allyl Compounds; D010636:Phenols
PIA; Staphylococcus aureus; biofilm; eDNA; honokiol