Fibroblasts profiling in scarring trachoma identifies IL-6 as a functional component of a fibroblast-macrophage pro-fibrotic and pro-inflammatory feedback loop.
Trachoma is a conjunctiva scarring disease, which is the leading infectious cause of blindness worldwide. Yet, the molecular mechanisms underlying progressive fibrosis in trachoma are unknown. To investigate the contribution of local resident fibroblasts to disease progression, we isolated conjunctival fibroblasts from patients with scarring trachoma and matching control individuals, and compared their gene expression profiles and functional properties in vitro. We show that scarring trachoma fibroblasts substantially differ from control counterparts, displaying pro-fibrotic and pro-inflammatory features matched by an altered gene expression profile. This pro-inflammatory signature was exemplified by increased IL-6 expression and secretion, and a stronger response to macrophage-mediated stimulation of contraction. We further demonstrate that scarring trachoma fibroblasts can promote Akt phosphorylation in macrophages in an IL-6 -dependent manner. Overall this work has uncovered a distinctive molecular fingerprint for scarring trachoma fibroblasts, and identified IL-6- as a potential contributor to the chronic conjunctival fibrosis, mediating reciprocal pro-fibrotic/pro-inflammatory interactions between macrophages and fibroblasts.
Department of Cell Biology, UCL Institute of Ophthalmology, 11-43 Bath Street, London, EC1V 9EL, UK.
Jenny Z
JZ
Kechagia
Department of Cell Biology, UCL Institute of Ophthalmology, 11-43 Bath Street, London, EC1V 9EL, UK.|Moorfields Eye Hospital, and UCL Partners AHSC, 11-43 Bath Street, London, EC1V 9EL, UK.
Daniel G
DG
Ezra
Moorfields Eye Hospital, and UCL Partners AHSC, 11-43 Bath Street, London, EC1V 9EL, UK.|International Centre for Eye Health, London School of Hygiene &Tropical Medicine, Keppel Street, London, WC1E 7HT, UK.
Matthew J
MJ
Burton
Department of Cell Biology, UCL Institute of Ophthalmology, 11-43 Bath Street, London, EC1V 9EL, UK.
Maryse
M
Bailly
D002921:Cicatrix / Q000378:metabolism* / Q000473:pathology; D003228:Conjunctiva / Q000378:metabolism / Q000473:pathology; D005260:Female; D005347:Fibroblasts / Q000378:metabolism* / Q000473:pathology; D005355:Fibrosis; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism* / Q000473:pathology; D008297:Male; D015398:Signal Transduction; D014141:Trachoma / Q000378:metabolism* / Q000473:pathology; D020298:U937 Cells
C508600:IL6 protein, human; D015850:Interleukin-6