Increased miR-381a-3p Contributes to Osteoarthritis by Targeting IkBα.
Osteoarthritis (OA) affects over 100 million individuals around the world. In this study, we mainly explore the functional role of miR-381a-3p in the regulation of OA. The expression of miR-381a-3p was studied in human articular cartilage and synovium of OA. Furthermore, the level of miR-381a-3p was analyzed in the OA rat model. MiR-381a-3p was overexpressed or inhibited with specific miR mimics or inhibitors. RT-PCR was applied to determine the levels of inflammatory factors such as TNFα, COX-2, iNOS, IL-6, and IL8. Bioinformatic predictions were applied to determine the possible target genes. Dual luciferase reporter assay was used to explore whether IκBα was the target gene of miR-381a-3p. The level of miR-381a-3p was significantly increased in the human articular cartilage and synovium of OA compared with normal control. Meanwhile, miR-381a-3p was enhanced in the OA rat model. Overexpression of miR-381a-3p significantly enhanced the levels of TNFα, COX-2, iNOS, IL-6 and IL8. IκBα was predicted as the target gene of miR-381a-3p. Dual luciferase reporter assay showed that miR-381a-3p could significantly decrease the luciferase activity of pmirGL-IκBα-3'UTR. In summary, enhanced miR-381a-3p expression contributed to the injury of OA mainly by inhibiting the expression of IκBα.
Department of graduate, Xuzhou Medical University, Xuzhou, Jiangsu, China.
Silong
S
Xia
Department of graduate, Xuzhou Medical University, Xuzhou, Jiangsu, China.
Kang
K
Yan
Department of Orthopedic, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, China wangyehua151121@163.com.
Yehua
Y
Wang
D000818:Animals; D057809:HEK293 Cells; D006801:Humans; D007249:Inflammation / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D000072000:NF-KappaB Inhibitor alpha / Q000235:genetics* / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D010003:Osteoarthritis / Q000235:genetics*; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
C588249:MIRN381 microRNA, human; C000608180:MIRN381 microRNA, rat; D035683:MicroRNAs; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D000072000:NF-KappaB Inhibitor alpha