Depletion of autophagy receptor p62/SQSTM1 enhances the efficiency of gene delivery in mammalian cells.
Novel methods that increase the efficiency of gene delivery to cells will have many useful applications. Here, we report a simple approach involving depletion of p62/SQSTM1 to enhance the efficiency of gene delivery. The efficiency of reporter gene delivery was remarkably higher in p62-knockout murine embryonic fibroblast (MEF) cells compared with normal MEF cells. This higher efficiency was partially attenuated by ectopic expression of p62. Furthermore, siRNA-mediated knockdown of p62 clearly increased the efficiency of transfection of murine embryonic stem (mES) cells and human HeLa cells. These data indicate that p62 acts as a key regulator of gene delivery.
Graduate School of Frontier Biosciences, Osaka University, Suita, Japan.
Megumi
M
Tsuchiya
Graduate School of Frontier Biosciences, Osaka University, Suita, Japan.|Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, Kobe, Japan.
Hidesato
H
Ogawa
Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, Kobe, Japan.
Takako
T
Koujin
Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, Kobe, Japan.
Shouhei
S
Kobayashi
Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, Kobe, Japan.
Chie
C
Mori
Graduate School of Frontier Biosciences, Osaka University, Suita, Japan.|Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, Kobe, Japan.
Yasushi
Y
Hiraoka
Graduate School of Frontier Biosciences, Osaka University, Suita, Japan.|Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, Kobe, Japan.
Tokuko
T
Haraguchi
D000818:Animals; D005347:Fibroblasts / Q000378:metabolism; D055786:Gene Knockout Techniques; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D000066450:Mouse Embryonic Stem Cells / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D000071456:Sequestosome-1 Protein / Q000037:antagonists & inhibitors / Q000235:genetics*; D014162:Transfection / Q000379:methods*
D034741:RNA, Small Interfering; C099718:SQSTM1 protein, human; D000071456:Sequestosome-1 Protein
DNA beads; LC3; autophagy