Comprehensive pyrosequencing analysis of the bacterial microbiota of the skin of patients with seborrheic dermatitis.
Seborrheic dermatitis (SD) is a chronic inflammatory dermatologic condition in which erythema and itching develop on areas of the body with sebaceous glands, such as the scalp, face and chest. The inflammation is evoked directly by oleic acid, which is hydrolyzed from sebum by lipases secreted by skin microorganisms. Although the skin fungal genus, Malassezia, is thought to be the causative agent of SD, analysis of the bacterial microbiota of skin samples of patients with SD is necessary to clarify any association with Malassezia because the skin microbiota comprises diverse bacterial and fungal genera. In the present study, bacterial microbiotas were analyzed at non-lesional and lesional sites of 24 patients with SD by pyrosequencing and qPCR. Principal coordinate analysis revealed clear separation between the microbiota of non-lesional and lesional sites. Acinetobacter, Corynebacterium, Staphylococcus, Streptococcus and Propionibacterium were abundant at both sites. Propionibacterium was abundant at non-lesional sites, whereas Acinetobacter, Staphylococcus and Streptococcus predominated at lesional sites; however, the extent of Propionibacterium colonization did not differ significantly between lesional and non-lesional sites according to qPCR. Given that these abundant bacteria hydrolyze sebum, they may also contribute to SD development. To the best of our knowledge, this is the first comprehensive analysis of the bacterial microbiotas of the skin of SD patients.
Department of Microbiology, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo, 204-8588.
Akiomi
A
Tanaka
Department of Microbiology, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo, 204-8588.
Otomi
O
Cho
Department of Microbiology, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo, 204-8588.
Chie
C
Saito
Department of Dermatology, Tokyo Medical University, 6-7-1 Nishi-Shinjuku, Tokyo, 160-0023, Japan.
Mami
M
Saito
Department of Dermatology, Tokyo Medical University, 6-7-1 Nishi-Shinjuku, Tokyo, 160-0023, Japan.
Ryoji
R
Tsuboi
Department of Microbiology, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo, 204-8588.
Takashi
T
Sugita
D000328:Adult; D000368:Aged; D044822:Biodiversity; D012628:Dermatitis, Seborrheic / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D054892:Metagenome; D056186:Metagenomics / Q000379:methods; D064307:Microbiota; D008875:Middle Aged; D017422:Sequence Analysis, DNA; D012867:Skin / Q000382:microbiology*; D055815:Young Adult
comprehensive analysis; seborrheic dermatitis; skin microbiota