Complete genome sequence of a novel sub-subgenotype 2.1g isolate of classical swine fever virus from China.
Current subgenotype 2.1 isolates of classical swine fever virus (CSFV) play a dominant role in CSF outbreaks in China, and a novel sub-subgenotype 2.1g of CSFV was recently identified, but the complete genome sequence of this new sub-subgenotype has not been reported. In this study, complete genome of 2.1g isolate GD19/2011 collected from Guangdong province of China in 2011 was sequenced. It was found to be 12,298 nucleotides (nt) in length, including a 375-nt 5'UTR, a 11,697-nt opening reading frame (ORF), and a 227-nt 3'UTR. GD19/2011 shared 91.0-93.7 % and 95.6-97.5 % nt and amino acid sequence identity, respectively, with other subgenotype 2.1 isolates. The topology of a phylogenetic tree constructed based on complete genome sequences of GD19/2011 and other CSFV isolates was identical to that obtained with full-length E2 gene sequences, but it was significantly different from those obtained with the 5'UTR and core sequences. Serial passages of GD9/2011 in PK-15 cells generated a highly cell-adapted virus stock with an infectious titer of 10(7.8) TCID50/ml at the 12(th) passage in which two amino acid substitutions, S476R and N2494S, were observed in comparison with the complete polyprotein sequence of the original isolate from kidney tissue, GD19/2011. This is the first report of the complete genome sequence of a 2.1g isolate, and the GD19/2011 isolate will be useful for further analysis of the evolution and virulence of CSFV isolates.
Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, Changchun, China.|Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA.
Wenjie
W
Gong
Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, Changchun, China.|College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.
Li
L
Zhang
College of Life Science, Foshan University, Foshan, China.
Zongji
Z
Lu
Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, Changchun, China.
Junjie
J
Jia
Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, Changchun, China.
Meng
M
Wang
Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, Changchun, China.
Zhicheng
Z
Peng
Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, Changchun, China.
Huancheng
H
Guo
Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA. jshi@ksu.edu.
Jishu
J
Shi
Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, Changchun, China. changchun_tu@hotmail.com.|Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China. changchun_tu@hotmail.com.
Changchun
C
Tu
D000818:Animals; D002681:China / Q000453:epidemiology; D006691:Classical Swine Fever / Q000453:epidemiology / Q000821:virology*; D006692:Classical Swine Fever Virus / Q000235:genetics*; D016679:Genome, Viral; D005838:Genotype; D010802:Phylogeny; D013552:Swine