Are Mucosa CD4+/CD8+ T-Cells Expressions Correlated with the Endoscopic Appearance of Chronic Gastritis Related with Helicobacter pylori Infection?
Local inflammatory processes in the gastric mucosa are followed by extensive immune cell infiltration, resulting in chronic active gastritis characterized by a marked infiltration of T(h)1 cytokine-producing CD4+ and CD8+T-cells Objective. To investigate the correlation between CD4+/CD8+ T-cells in gastric mucosa with endoscopic appearance in chronic gastritis with or without H.pylori infection. Prospective, cross sectional study is performed in a chronic dyspepsia population in July-November 2009 at Dr. Sardjito General Hospital Yogyakarta, Indonesia. The update Sydney system was used to analyze the gastroscopy appearance. Biopsy specimens were stained with HE-stain and IHC-stain. Data were analyzed by t-test, Mann-Whitney and Spearman correlation test. Number of 88 consecutive subjects are enrolled the study (50% male; 50% female), age 46±15 years; 25% H.pylori positive. The expression of CD4+ and CD8+ were higher in H.pylori negative subjects, but only the CD4+ was significant (P=0.011). A significant correlation was found between CD4+ and CD8+ in both subjects (r(Hp+)=0.62 and r(Hp-)=0.68; P<0.05). The expression of CD4+ and CD8+ in H.pylori positive showed a significant correlation with gastric lesions (r(CD4+)=-0.60; r(CD8+)=-0.42 ; P<0.05), only erosion showed a significant difference in both subjects. A positive correlation was found between CD4+ and CD8+ infiltration in both subjects with or without H.pylori infection, and a negative correlation was only found between gastric lesion with CD4+ and CD8+ infiltration in H.pylori subject.
Department of Internal Medicine, Dr. Sardjito General Hospital, Gadjah Mada University, Yogyakarta, Indonesia.
Neneng
N
Ratnasari
Department of Internal Medicine, Dr. Sardjito General Hospital, Gadjah Mada University, Yogyakarta, Indonesia.
Putut
P
Bayupurnama
Department of Internal Medicine, Dr. Sardjito General Hospital, Gadjah Mada University, Yogyakarta, Indonesia.
Sutanto
S
Maduseno
Department of Internal Medicine, Dr. Sardjito General Hospital, Gadjah Mada University, Yogyakarta, Indonesia.
Fahmi
F
Indrarti
Department of Internal Medicine, Dr. Sardjito General Hospital, Gadjah Mada University, Yogyakarta, Indonesia.
Catharina
C
Triwikatmani
Department of Pathology, School of Medicine, Gadjah Mada University, Yogyakarta, Indonesia.
Achmad
A
Harijadi
Department of Internal Medicine, Dr. Sardjito General Hospital, Gadjah Mada University, Yogyakarta, Indonesia.
Siti
S
Nurdjanah
D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002908:Chronic Disease; D003430:Cross-Sectional Studies; D005260:Female; D005753:Gastric Mucosa / Q000276:immunology / Q000473:pathology; D005756:Gastritis / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D005773:Gastroscopy; D016481:Helicobacter Infections / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies
CD4+/CD8+ T-cells; Chronic gastritis; Gastric lesion; H. pylori