Protective effects of naringin against gp120-induced injury mediated by P2X7 receptors in BV2 microglial cells.
This study was aimed at exploring the effects of P2X7 receptors on gp120-induced injury and naringin's protective effects against gp120-induced injury in BV2 microglia. BV2 microglia injury model was established by gp120 treatment and MTS assay was used to verify whether naringin has a cell-protective effect against gp120-induced injury. Changes in P2X7 receptor expression were assayed using RT-PCR, qPCR, and western blot. Results showed that the ODs of the Ctrl, gp120, gp120+naringin, and gp120+BBG groups were 0.91 ± 0.10, 0.71 ± 0.09, 0.83 ± 0.10, and 0.83 ± 0.10, respectively. Compared to the control group, the gp120 group showed a significantly decreased cell survival rate. Cell survival rates of the gp120+naringin group increased significantly compared to those of the gp120 group, while no difference was observed when compared to the gp120+BBG group. The relative P2X7 mRNA expression levels in the Ctrl, gp120, gp120+naringin, and gp120+BBG groups were 0.73 ± 0.06, 1.05 ± 0.06, 0.78 ± 0.05, and 0.81 ± 0.04, respectively. The corresponding P2X7 protein expression levels were 0.46 ± 0.04, 0.79 ± 0.04, 0.38 ± 0.07, and 0.42 ± 0.06. P2X7 mRNA and protein expression in the gp120 group increased significantly compared to those in the control group, and declined in the gp120+naringin group compared to those in the gp120 group. Therefore, P2X7 receptors might be involved in gp120-induced injury in BV2 microglia, and naringin might play a protective role by inhibiting the up-regulated expression of P2X7 receptors.
Department of Physiology, Basic Medical College, Nanchang University, Nanchang, China.
Q
Q
Chen
The First Clinical Medical College, Nanchang University, Nanchang, China.
J
J
Hu
Department of Physiology, Basic Medical College, Nanchang University, Nanchang, China.
S S
SS
Qin
Department of Physiology, Basic Medical College, Nanchang University, Nanchang, China.
C L
CL
Liu
The Second Clinical Medical College, Nanchang University, Nanchang, China.
H
H
Wu
The First Clinical Medical College, Nanchang University, Nanchang, China.
J R
JR
Wang
The First Clinical Medical College, Nanchang University, Nanchang, China.
X M
XM
Lu
The First Clinical Medical College, Nanchang University, Nanchang, China.
J
J
Wang
The First Clinical Medical College, Nanchang University, Nanchang, China.
G Q
GQ
Chen
The First Clinical Medical College, Nanchang University, Nanchang, China.
Y
Y
Liu
The First Clinical Medical College, Nanchang University, Nanchang, China.
B Y
BY
Liu
Department of Physiology, Basic Medical College, Nanchang University, Nanchang, China.
C S
CS
Xu
Department of Physiology, Basic Medical College, Nanchang University, Nanchang, China.
S D
SD
Liang
D015526:AIDS Dementia Complex / Q000188:drug therapy / Q000378:metabolism / Q000821:virology; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D044950:Flavanones / Q000494:pharmacology*; D015699:HIV Envelope Protein gp120 / Q000633:toxicity*; D051379:Mice; D017628:Microglia / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D058486:Receptors, Purinergic P2X7 / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D015854:Up-Regulation
D044950:Flavanones; D015699:HIV Envelope Protein gp120; D012333:RNA, Messenger; D058486:Receptors, Purinergic P2X7; C514793:gp120 protein, Human immunodeficiency virus 1; C005274:naringin