The Effect of Ozonated Olive Oil on Neovascularizatıon in an Experimental Skin Flap Model.
OBJECTIVE
Angiogenesis is an important factor for flap viability. It has been reported that ozonated oil contributed to improved neovascularization in an acute cutaneous wound healing model. This study was undertaken to evaluate the effect of ozonated olive oil on vascular endothelial growth factor (VEGF)-mediated neovascularization of skin flaps in rats.


METHODS
A skin flap model was established in 21 rats and evaluated within 3 groups. No treatment was given to the rats in group 1. Olive oil and ozonated olive oil were topically applied (twice daily) to the flap surface for 7 days in groups 2 and 3, respectively. Immunohistochemical staining was performed to analyze the expressions of VEGF and CD34.


RESULTS
The mean numbers of VEGF- and CD34-positive staining microvascular structures were 8.86 (SD, 1.35) and 10.29 (SD, 1.80) in group 1, 15.00 (SD, 1.41) and 15.57 (SD, 1.72) in group 2, and 25.14 (SD, 2.41) and 25.00 (SD, 2.16) in group 3. The VEGF and CD34 expressions in group 3 were significantly higher than those in group 2 (P < .001). Their expressions in group 2 were significantly higher than those in group 1 (P < .001).


CONCLUSIONS
Both ozonated olive oil and olive oil improved neovascularization when they were topically applied on skin flaps. The effect of ozone was more prominent.
Cüneyt Kırkıl, MD, is an Associate Professor, and Mehmet Volkan Yiğit, MD, is a Resident, Medical Faculty, Department of General Surgery, Firat University, Elazığ, Turkey. İbrahim Hanifi Özercan, MD, is a Professor, Department of Pathology, Medical Faculty, Firat University, Elazığ, Turkey. Erhan Aygen, MD, is a Professor, Department of General Surgery, Medical Faculty, Firat University, Elazığ, Turkey. Barış Gültürk, MD, is a Specialist, Department of General Surgery, Harput State Hospital, Elazığ, Turkey. Gökhan Artaş, MD, is a Specialist, Department of Pathology, Medical Faculty, Firat University, Elazığ, Turkey.
Cüneyt
C
Krkl
Mehmet Volkan
MV
Yiğit
İbrahim Hanifi
İH
Özercan
Erhan
E
Aygen
Barş
B
Gültürk
Gökhan
G
Artaş
D000287:Administration, Topical; D000704:Analysis of Variance; D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis; D016001:Confidence Intervals; D004195:Disease Models, Animal; D008297:Male; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D000069463:Olive Oil / Q000008:administration & dosage*; D010126:Ozone; D008517:Phytotherapy / Q000379:methods; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D016038:Skin Transplantation; D013524:Surgical Flaps; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D014947:Wounds and Injuries / Q000188:drug therapy / Q000601:surgery*
D018952:Antigens, CD34; D015415:Biomarkers; D000069463:Olive Oil; D042461:Vascular Endothelial Growth Factor A; D010126:Ozone