Oxygenated monoterpenes-rich volatile oils as potential antifungal agents for dermatophytes.
Essential oils (EOs) extracted from Lavandula luisieri and Cymbopogon citratus were tested for their antifungal activity against ten clinical isolates of dermatophytes isolated from cases of tinea pedis. Inhibition of conidial germination and antifungal drug/EO combination assay were tested on two ATCC reference strains of Trichophyton rubrum and Trichophyton mentagrophytes. EOs were characterised by high amount of oxygenated monoterpenes in their composition. Strong antifungal activity was observed for the majority of clinical strains, and fungicidal activity was demonstrated. Positive interaction between L. luisieri EO combined with terbinafine was observed against terbinafine-resistant strain (Tr ATCC MYA-4438). Significative reduction of the germination was observed above 100 μg mL-1. Both oils were safe to macrophage mammalian cells at tested concentration. This study describes the antifungal activity of L. luisieri and C. citratus EOs against dermatophytes, which could be useful in designing new formulations for topical treatments.
a CEB - Centre of Biological Engineering , University of Minho, Campus Gualtar , Braga , Portugal.
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b CESPU - Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde , Gandra , Portugal.
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c CEF - Centro de Estudos Farmacêuticos/Faculty of Pharmacy , University of Coimbra , Coimbra , Portugal.
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c CEF - Centro de Estudos Farmacêuticos/Faculty of Pharmacy , University of Coimbra , Coimbra , Portugal.|d CNC - Center for Neuroscience and Cell Biology , University of Coimbra , Coimbra , Portugal.
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a CEB - Centre of Biological Engineering , University of Minho, Campus Gualtar , Braga , Portugal.
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b CESPU - Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde , Gandra , Portugal.|c CEF - Centro de Estudos Farmacêuticos/Faculty of Pharmacy , University of Coimbra , Coimbra , Portugal.|e CIBIO - Research Centre in Biodiversity and Genetic Resources, InBIO , Universidade do Porto , Campus Agrário de Vairão, Vila do Conde , Portugal.
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D000935:Antifungal Agents / Q000494:pharmacology*; D003883:Arthrodermataceae / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D039821:Monoterpenes / Q000494:pharmacology*; D009822:Oils, Volatile / Q000494:pharmacology*; D010100:Oxygen; D014249:Trichophyton / Q000187:drug effects
D000935:Antifungal Agents; D039821:Monoterpenes; D009822:Oils, Volatile; D010100:Oxygen
Antifungals; dermatophytes; pathogenic fungi; skin infections