Hydraulic retention time affects stable acetate production from tofu processing wastewater in extreme-thermophilic (70°C) mixed culture fermentation.
Acetate is an important industrial chemical and its production from wastes via mixed culture fermentation (MCF) is economic. In this work, the effect of hydraulic retention time (HRT) on acetate production from tofu processing wastewater (TPW) in extreme-thermophilic (70°C) MCF was first investigated. It was found that long HRT (>3days) could lead to less acetate production while stable acetate production was achieved at short HRT (3days) with the yield of 0.57g-COD/g-CODTPW. The microbial community analysis showed that hydrogenotrophic methanogens (mainly Methanothermobacter) occupied up to 90% of archaea at both HRTs of 3 and 5days. However, Coprothermobacter, the main acetate-degraders, decreased from 35.74% to 10.58% of bacteria when HRT decreased from 5 to 3days, supporting the aggravation of syntrophic acetate oxidation in long HRT. This work demonstrated that HRT was a crucial factor to maintain stable acetate production from TPW in extreme-thermophilic MCF.
CAS Key Laboratory of Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China.
Yun
Y
Chen
Hebei Key Laboratory of Applied Chemistry, School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, Hebei 066004, People's Republic of China.
Fang
F
Zhang
CAS Key Laboratory of Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China.
Ting
T
Wang
School of Environmental Engineering and Science, Yangzhou University, 196 West Huayang Road, Yangzhou, Jiangsu 225127, People's Republic of China.
Nan
N
Shen
CAS Key Laboratory of Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China.
Zhong-Wei
ZW
Yu
CAS Key Laboratory of Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China. Electronic address: rzeng@ustc.edu.cn.
Raymond J
RJ
Zeng
D000085:Acetates / Q000378:metabolism*; D001419:Bacteria / Q000378:metabolism; D061249:Batch Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology*; D005285:Fermentation; D005525:Food-Processing Industry; D008697:Methane / Q000096:biosynthesis; D017013:Methanobacteriaceae / Q000378:metabolism; D059013:Microbial Consortia; D045730:Soy Foods; D014865:Waste Disposal, Fluid / Q000379:methods*; D062065:Wastewater / Q000737:chemistry*
D000085:Acetates; D008697:Methane; D062065:Wastewater
Extreme-thermophilic mixed culture fermentation; Hydraulic retention time; Stable acetate production; Syntrophic acetate oxidation; Tofu processing wastewater