The cellular lipids of Romboutsia.
We have examined the lipids of three isolates, Romboutsia lituseburensis, Romboutsia ilealis, and Romboutsia sp. strain FRIFI, of the newly described genus Romboutsia by two-dimensional thin-layer chromatography (2D-TLC) and by liquid chromatography/mass spectrometry (LC/MS). We have found three phospholipids, phosphatidylglycerol (PG), cardiolipin and phosphatidic acid in all three species. A fourth phospholipid, lysyl-PG, was found in R. lituseburensis and strain FRIFI. Polyprenyl-phosphates were identified in the lipid extracts of all three species. Three glycolipids, mono-, di- and tri-hexosyldiacylglycerol, were common to all three species. An additional glycolipid, tetrahexosyl-diacylglycerol was identified in strain FRIFI. Acylated trihexosyldiacylglycerol and acyl-tetrahexosydiacylglycerol were also found in R. ilealis and strain FRIFI. Remarkably, no alk-1-enyl ether lipids (plasmalogens) were present in Romboutsia as distinct from bacteria of the related genus Clostridium in which these ether lipids are common. We have compared the lipidome of Romboutsia with that recently described for Clostridium difficile, which has plasmalogens, no lysyl-PG, and no tetrahexosyl-diacylglycerol. According to 16S rRNA gene sequencing, Romboutsia spp. and C. difficile are closely related (>95% sequence identity).
Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.
Ziqiang
Z
Guan
Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104-6076, USA.
Lingli
L
Chen
Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands; Winclove Probiotics B.V., Amsterdam, The Netherlands.
Jacoline
J
Gerritsen
Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands.
Hauke
H
Smidt
Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104-6076, USA. Electronic address: goldfinh@mail.med.upenn.edu.
Howard
H
Goldfine
D002308:Cardiolipins / Q000302:isolation & purification / Q000378:metabolism; D002853:Chromatography, Liquid; D003013:Clostridium / Q000235:genetics / Q000378:metabolism*; D005227:Fatty Acids / Q000302:isolation & purification / Q000378:metabolism; D008055:Lipids / Q000235:genetics / Q000302:isolation & purification*; D013058:Mass Spectrometry; D010712:Phosphatidic Acids / Q000302:isolation & purification / Q000378:metabolism; D010715:Phosphatidylglycerols / Q000302:isolation & purification / Q000378:metabolism*; D010743:Phospholipids / Q000302:isolation & purification / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D002308:Cardiolipins; D005227:Fatty Acids; D008055:Lipids; D010712:Phosphatidic Acids; D010715:Phosphatidylglycerols; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S
Glycolipid; Mass spectrometry; Phospholipid; Romboutsia; Thin-layer chromatography