BAFF Expression is Modulated by Female Hormones in Human Immune Cells.
Among several autoimmune diseases, one of the main risk factors is the female gender, and much consideration has been given to the involvement of female hormones in their etiology. B-cell activating factor (BAFF) is a key factor in survival and maturation of B cells and is overexpressed in several autoimmune patients although the mechanism behind this feature is unclear. In murine models, BAFF expression could be upregulated by exogenous estrogen treatment in splenocytes; however, no evidence of this relationship was available in humans. Here, leukocytes from healthy male and female individuals were collected and cultivated in the presence or absence of estrogen or progesterone. BAFF gene expression was accessed by quantitative PCR and compared between treated and untreated group of cells. In the presence of estrogen, BAFF expression was upregulated by more than 5 times in both genders. When exposed to progesterone, the female-originated cells showed increased expression, while the cells of male origin a significant downregulation of BAFF. Our results suggest that female hormones can modulate the expression of BAFF, a key cytokine in autoimmune pathways, in human immune cells. These data might contribute to the understanding of the etiology as well as the gender bias featured by several autoimmune disorders.
Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, Florianopolis, Brazil.
Manuela N
MN
Drehmer
Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, Florianopolis, Brazil.
Dalila G
DG
Suterio
Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, Florianopolis, Brazil.
Yara C N
YC
Muniz
Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, Florianopolis, Brazil.
Iliada R
IR
de Souza
Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, Florianopolis, Brazil. sara.emelie@gmail.com.
Sara E
http://orcid.org/0000-0002-1680-7349
SE
Löfgren
D000328:Adult; D000818:Animals; D053264:B-Cell Activating Factor / Q000235:genetics*; D002478:Cells, Cultured; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007962:Leukocytes / Q000166:cytology* / Q000187:drug effects; D008297:Male; D051379:Mice; D011374:Progesterone / Q000494:pharmacology*; D015854:Up-Regulation; D055815:Young Adult
D053264:B-Cell Activating Factor; D004967:Estrogens; C505719:TNFSF13B protein, human; D011374:Progesterone
Autoimmune disease; BAFF; Expertise in immunology; Female hormones; Gene expression; Immunogenetics