Ectopic expression of Arabidopsis thaliana Na+(K+)/H+ antiporter gene, AtNHX5, enhances soybean salt tolerance.
Drought and salt stresses are the two major factors influencing the yield and quality of crops worldwide. Na(+)(K(+))/H(+) antiporters (NHXs) are ubiquitous membrane proteins that play important roles in maintaining the cellular pH and Na(+)(K(+)) homeostasis. The model plant Arabidopsis potentially encodes six NHX genes, namely AtNHX1 to 6. In the present study, AtNHX5, a comparatively less well-studied NHX, was cloned and transferred into a soybean variety, Dongnong-50, via Agrobacterium-mediated cotyledonary node transformation to assess its role in improving salt tolerance of the transgenic plants. The transgenic soybean plants were tolerant to the presence of 300 mM NaCl whereas the non-transgenic plants were not. Furthermore, after NaCl treatment, the transgenic plants had a higher content of free proline but lower content of malondialdehyde compared to the non-transgenic plants. Our results revealed that that AtNHX5 possibly functioned by efficiently transporting Na(+) and K(+) ions from the roots to the leaves. Overall, the results obtained in this study suggest that soybean salt tolerance could be improved through the over expression of Arabidopsis AtNHX5.
Key Laboratory of Soybean Biology of Chinese Ministry of Education, College of Agronomy, Northeast Agricultural University, Harbin, Heilongjiang, China.
X X
XX
Wu
Key Laboratory of Soybean Biology of Chinese Ministry of Education, College of Agronomy, Northeast Agricultural University, Harbin, Heilongjiang, China.
J
J
Li
Key Laboratory of Soybean Biology of Chinese Ministry of Education, College of Agronomy, Northeast Agricultural University, Harbin, Heilongjiang, China.
X D
XD
Wu
Institute of Horticultural Sciences, Heilongjiang Academy of Agricultural Science, Harbin, Heilongjiang, China.
Q
Q
Liu
Key Laboratory of Soybean Biology of Chinese Ministry of Education, College of Agronomy, Northeast Agricultural University, Harbin, Heilongjiang, China.
Z K
ZK
Wang
Key Laboratory of Soybean Biology of Chinese Ministry of Education, College of Agronomy, Northeast Agricultural University, Harbin, Heilongjiang, China.
S S
SS
Liu
Key Laboratory of Soybean Biology of Chinese Ministry of Education, College of Agronomy, Northeast Agricultural University, Harbin, Heilongjiang, China.
S N
SN
Li
Key Laboratory of Soybean Biology of Chinese Ministry of Education, College of Agronomy, Northeast Agricultural University, Harbin, Heilongjiang, China.
Y L
YL
Ma
Berries Research Institute, Heilongjiang Academy of Agricultural Sciences, Suiling, Heilongjiang, China.
J
J
Sun
Key Laboratory of Soybean Biology of Chinese Ministry of Education, College of Agronomy, Northeast Agricultural University, Harbin, Heilongjiang, China.
L
L
Zhao
Key Laboratory of Soybean Biology of Chinese Ministry of Education, College of Agronomy, Northeast Agricultural University, Harbin, Heilongjiang, China.
H Y
HY
Li
Key Laboratory of Soybean Biology of Chinese Ministry of Education, College of Agronomy, Northeast Agricultural University, Harbin, Heilongjiang, China.
D M
DM
Li
Key Laboratory of Soybean Biology of Chinese Ministry of Education, College of Agronomy, Northeast Agricultural University, Harbin, Heilongjiang, China.
W B
WB
Li
College of Resources and Environment, Northeast Agricultural University, Harbin, Heilongjiang, China.
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AY
Su
D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D055864:Droughts; D000066630:Ectopic Gene Expression; D018506:Gene Expression Regulation, Plant; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D055049:Salt Tolerance / Q000235:genetics*; D013025:Glycine max / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
D029681:Arabidopsis Proteins; C000609621:NHX5 protein, Arabidopsis