Quantification of Lung Metastases from In Vivo Mouse Models.
Cancer research has made significant progress in terms of understanding and targeting primary tumors; however, the challenge remains for the successful treatment of metastatic cancers. This highlights the importance to use in vivo models to study the metastatic process, as well as for preclinical testing of compounds that could inhibit metastasis. As a result, proper quantification of metastases from in vivo models is of the utmost significance. Here, we provide a detailed protocol for collecting and handling lung tissues from mice, and guidance for subsequent analysis of metastases, as well as interpretation of data.
Biotech Research & Innovation Centre (BRIC), University of Copenhagen, Ole Maaløes Vej 5, 2200, Copenhagen, Denmark.|Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill, 27599, Chapel Hill, NC, USA.
Joan
J
Chang
Biotech Research & Innovation Centre (BRIC), University of Copenhagen, Ole Maaløes Vej 5, 2200, Copenhagen, Denmark. Janine.erler@bric.ku.dk.
Janine T
JT
Erler
D000818:Animals; D004195:Disease Models, Animal; D007091:Image Processing, Computer-Assisted; D008168:Lung / Q000473:pathology; D008175:Lung Neoplasms / Q000556:secondary*; D051379:Mice
ImageJ; In vivo model; Metastasis; Quantification; Tissue collection