Effect of the transdermal low-level laser therapy on endothelial function.
The effect of low-level laser therapy (LLLT) on the cardiovascular system is not fully established. Since the endothelium is an important endocrine element, establishing the mechanisms of LLLT action is an important issue.The aim of the study was to evaluate the effect of transdermal LLLT on endothelial function.In this study, healthy volunteers (n = 40, age = 20-40 years) were enrolled. N = 30 (14 female, 16 male, mean age 30 ± 5 years) constituted the laser-irradiated group (LG). The remaining 10 subjects (6 women, 4 men, mean age 28 ± 5 years) constituted the control group (CG). Participants were subjected to LLLT once a day for three consecutive days. Blood for biochemical assessments was drawn before the first irradiation and 24 h after the last session. In the LG, transdermal illumination of radial artery was conducted (a semiconductor laser λ = 808 nm, irradiation 50 mW, energy density 1.6 W/cm(2) and a dose 20 J/day, a total dose of 60 J). Biochemical parameters (reflecting angiogenesis: vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF), angiostatin; antioxidative status: glutathione (GSH) and the nitric oxide metabolic pathway: symmetric dimethylarginine (SDMA), asymmetric dimethylarginine (ADMA) and L-arginine) were assessed. In the LG, a significant increase in GSH levels and considerable decrease in angiostatin concentration following the LLLT were observed. No significant differences in levels of the VEGF, FGF, SDMA, ADMA were observed.LLLT modifies vascular endothelial function by increasing its antioxidant and angiogenic potential. We found no significant differences in levels of the nitric oxide pathway metabolites within 24 h following the LLLT irradiation.
Department of Internal and Occupational Diseases, Hypertension and Clinical Oncology, Wroclaw Medical University, Borowska 213 Street, 50-552, Wroclaw, Poland.|Research and Development Department, Wrovasc - Integrated Cardiovascular Centre Provincial Specialist Hospital in Wroclaw, Kamienskiego 73a Street, 51-124, Wroclaw, Poland.
Alicja
A
Szymczyszyn
Department of Internal and Occupational Diseases, Hypertension and Clinical Oncology, Wroclaw Medical University, Borowska 213 Street, 50-552, Wroclaw, Poland.|Research and Development Department, Wrovasc - Integrated Cardiovascular Centre Provincial Specialist Hospital in Wroclaw, Kamienskiego 73a Street, 51-124, Wroclaw, Poland.
Adrian
A
Doroszko
Department of Internal and Occupational Diseases, Hypertension and Clinical Oncology, Wroclaw Medical University, Borowska 213 Street, 50-552, Wroclaw, Poland.|Research and Development Department, Wrovasc - Integrated Cardiovascular Centre Provincial Specialist Hospital in Wroclaw, Kamienskiego 73a Street, 51-124, Wroclaw, Poland.
Ewa
E
Szahidewicz-Krupska
Research and Development Department, Wrovasc - Integrated Cardiovascular Centre Provincial Specialist Hospital in Wroclaw, Kamienskiego 73a Street, 51-124, Wroclaw, Poland.
Piotr
P
Rola
Research and Development Department, Wrovasc - Integrated Cardiovascular Centre Provincial Specialist Hospital in Wroclaw, Kamienskiego 73a Street, 51-124, Wroclaw, Poland.
Radosław
R
Gutherc
Research and Development Department, Wrovasc - Integrated Cardiovascular Centre Provincial Specialist Hospital in Wroclaw, Kamienskiego 73a Street, 51-124, Wroclaw, Poland.
Jakub
J
Jasiczek
Department of Internal and Occupational Diseases, Hypertension and Clinical Oncology, Wroclaw Medical University, Borowska 213 Street, 50-552, Wroclaw, Poland.
Grzegorz
G
Mazur
Department of Internal and Occupational Diseases, Hypertension and Clinical Oncology, Wroclaw Medical University, Borowska 213 Street, 50-552, Wroclaw, Poland. aderkacz@wp.pl.|Research and Development Department, Wrovasc - Integrated Cardiovascular Centre Provincial Specialist Hospital in Wroclaw, Kamienskiego 73a Street, 51-124, Wroclaw, Poland. aderkacz@wp.pl.
Arkadiusz
A
Derkacz
D000328:Adult; D043168:Angiostatins / Q000528:radiation effects; D001120:Arginine / Q000031:analogs & derivatives / Q000528:radiation effects; D005260:Female; D005346:Fibroblast Growth Factors / Q000528:radiation effects; D005978:Glutathione / Q000528:radiation effects; D006801:Humans; D054023:Lasers, Semiconductor / Q000627:therapeutic use*; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D009569:Nitric Oxide / Q000528:radiation effects; D042461:Vascular Endothelial Growth Factor A / Q000528:radiation effects*
D042461:Vascular Endothelial Growth Factor A; D009569:Nitric Oxide; C024917:symmetric dimethylarginine; D005346:Fibroblast Growth Factors; C018524:N,N-dimethylarginine; D043168:Angiostatins; D001120:Arginine; D005978:Glutathione
Low-level laser therapy (LLLT); Nitric oxide metabolism; Oxidative stress; Vascular endothelium