Oligonucleotide-based label-free detection with optical microresonators: strategies and challenges.
This review targets diversified oligonucleotide-based biodetection techniques, focusing on the use of microresonators of whispering gallery mode (WGM) type as optical biosensors mostly integrated with lab-on-a-chip systems. On-chip and microfluidics combined devices along with optical microresonators provide rapid, robust, reproducible and multiplexed biodetection abilities in considerably small volumes. We present a detailed overview of the studies conducted so far, including biodetection of various oligonucleotide biomarkers as well as deoxyribonucleic acids (DNAs), ribonucleic acids (RNAs) and proteins. We particularly advert to chemical surface modifications for specific and selective biosensing.
Institute of Materials Science and Nanotechnology, Bilkent University, 06800 Ankara, Turkey. bayindir@nano.org.tr and UNAM-National Nanotechnology Research Center, Bilkent University, 06800 Ankara, Turkey.
Pelin
P
Toren
Institute of Materials Science and Nanotechnology, Bilkent University, 06800 Ankara, Turkey. bayindir@nano.org.tr and UNAM-National Nanotechnology Research Center, Bilkent University, 06800 Ankara, Turkey.
Erol
E
Ozgur
Institute of Materials Science and Nanotechnology, Bilkent University, 06800 Ankara, Turkey. bayindir@nano.org.tr and UNAM-National Nanotechnology Research Center, Bilkent University, 06800 Ankara, Turkey and Department of Physics, Bilkent University, 06800 Ankara, Turkey.
Mehmet
M
Bayindir
D052158:Aptamers, Peptide; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D004247:DNA / Q000032:analysis*; D019175:DNA Methylation; D004867:Equipment Design; D014644:Genetic Variation; D006801:Humans; D056656:Lab-On-A-Chip Devices; D009841:Oligonucleotides; D055095:Optics and Photonics / Q000295:instrumentation; D020135:Peptide Nucleic Acids / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D012313:RNA / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis
D052158:Aptamers, Peptide; D009841:Oligonucleotides; D020135:Peptide Nucleic Acids; D011506:Proteins; D012333:RNA, Messenger; D012313:RNA; D004247:DNA