A microRNA-152 that targets the phosphatase and tensin homolog to inhibit low oxygen induced-apoptosis in human brain microvascular endothelial cells.
Brain damage caused by perinatal asphyxia is dangerous for neonatal infants, but the mechanism by which it occurs remains elusive. In this study, microRNA-152 (miR-152) expression was induced by low oxygen levels in rat models of hypoxia brain damage, as well as in human brain microvascular endothelial cells (HBMECs) cultured in vitro. Analysis of the sequence of miR-152 revealed that the phosphatase and tensin homolog gene (PTEN) is probably the target of miR-152 both in humans and rats. When HBMECs were transfected with miR-152 mimics, PTEN expression was inhibited at both the mRNA and protein levels. Moreover, transfection with the miR-152 mimic also inhibited apoptosis induced by hypoxia. Furthermore, expression of the pro-apoptotic gene Bax was downregulated while the anti-apoptotic gene Bcl2 was upregulated after miR-152 mimic transfection. Taken together, these results indicate that miR-152 induced by hypoxia suppresses cell apoptosis and acts as a protective factor during hypoxia by repressing PTEN.
Department of Pediatrics, General Hospital of Military Command Jinan, Jinan, Shandong, China.
Y H
YH
Cao
Department of Pediatric Surgery, The Second Affiliated Hospital of Shandong University, Jinan, Shandong, China.
D G
DG
Li
Department of Pediatrics, General Hospital of Military Command Jinan, Jinan, Shandong, China.
B
B
Xu
Department of Pediatrics, General Hospital of Military Command Jinan, Jinan, Shandong, China.
M Q
MQ
Wang
Department of Pediatrics, General Hospital of Military Command Jinan, Jinan, Shandong, China.
N
N
Zhen
Department of Pediatrics, General Hospital of Military Command Jinan, Jinan, Shandong, China.
L X
LX
Man
Department of Pediatrics, General Hospital of Military Command Jinan, Jinan, Shandong, China.
Y Y
YY
Zhang
Department of Pediatrics, General Hospital of Military Command Jinan, Jinan, Shandong, China.
M
M
Chi
D000818:Animals; D017209:Apoptosis / Q000235:genetics; D001921:Brain / Q000098:blood supply; D015687:Cell Hypoxia / Q000502:physiology; D042783:Endothelial Cells / Q000201:enzymology* / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D002534:Hypoxia, Brain / Q000378:metabolism* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis*; D055806:Microvessels / Q000201:enzymology / Q000378:metabolism; D023421:Models, Animal; D010100:Oxygen / Q000378:metabolism*; D051059:PTEN Phosphohydrolase / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
C550856:MIRN152 microRNA, human; C578788:MIRN152 microRNA, rat; D035683:MicroRNAs; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human; C487216:Pten protein, rat; D010100:Oxygen