CXCR4-using HIV variants in a cohort of Black men who have sex with men: HIV Prevention Trials Network 061.
To evaluate factors associated with HIV tropism among Black men who have sex with men (MSM) in the United States enrolled in a clinical study (HIV Prevention Trials Network 061).



HIV tropism was analyzed using a phenotypic assay (Trofile assay, Monogram Biosciences). Samples were analyzed from 43 men who were HIV infected at enrollment and reported either exclusive insertive intercourse or exclusive receptive intercourse; samples were also analyzed from 20 men who were HIV uninfected at enrollment and seroconverted during the study. Clonal analysis of individual viral variants was performed for seroconverters who had dual/mixed (DM) viruses.



DM viruses were detected in samples from 11 (26%) of the 43 HIV-infected men analyzed at the enrollment visit; HIV tropism did not differ between those reporting exclusive insertive vs receptive intercourse. DM viruses were also detected in five (25%) of the 20 seroconverters. DM viruses were associated with lower CD4 cell counts. Seroconverters with DM viruses had dual-tropic viruses only or mixed populations of CCR5- and dual-tropic viruses.



DM viruses were frequently detected among Black MSM in this study, including seroconverters. Further studies are needed to understand factors driving transmission and selection of CXCR4- and dual-tropic viruses among Black MSM.
a Department of Pathology , Johns Hopkins University School of Medicine , Baltimore , MD , USA.
Iris
I
Chen
b Monogram Biosciences , South San Francisco , CA , USA.
Wei
W
Huang
c Vaccine and Infectious Disease Division , Fred Hutchinson Cancer Research Center , Seattle , WA , USA.
Matthew B
MB
Connor
b Monogram Biosciences , South San Francisco , CA , USA.
Arne
A
Frantzell
a Department of Pathology , Johns Hopkins University School of Medicine , Baltimore , MD , USA.
Vanessa
V
Cummings
c Vaccine and Infectious Disease Division , Fred Hutchinson Cancer Research Center , Seattle , WA , USA.
Geetha G
GG
Beauchamp
d Science Facilitation Department , FHI 360 , Durham , NC , USA.
Sam
S
Griffith
e Mervyn M. Dymally School of Nursing , Charles R. Drew University of Medicine and Science , Los Angeles , CA , USA.
Sheldon D
SD
Fields
f Bridge HIV , San Francisco Department of Public Health , San Francisco , CA , USA.
Hyman M
HM
Scott
g Department of Family Medicine , University of California Los Angeles , Los Angeles , CA , USA.
Steven
S
Shoptaw
h Department of Global Health , Emory University Rollins School of Public Health , Atlanta , GA , USA.
Carlos
C
Del Rio
i Department of Epidemiology and Biostatistics , The George Washington University , Washington , DC , USA.
Manya
M
Magnus
j Department of Medicine, Harlem Hospital , Columbia University Mailman School of Public Health , New York , NY , USA.
Sharon
S
Mannheimer
k Laboratory of Infectious Disease Prevention , Lindsley F. Kimball Research Institute, New York Blood Center , New York , NY , USA.
Hong-Van
HV
Tieu
l School of Social Welfare , University at Albany, State University of New York , Albany , NY , USA.
Darrell P
DP
Wheeler
m The Fenway Institute , Fenway Health , Boston , MA , USA.|n Infectious Disease Division , Beth Israel Deaconess Medical Center , Boston , MA , USA.|o Department of Medicine , Harvard Medical School , Boston , MA , USA.
Kenneth H
KH
Mayer
k Laboratory of Infectious Disease Prevention , Lindsley F. Kimball Research Institute, New York Blood Center , New York , NY , USA.
Beryl A
BA
Koblin
a Department of Pathology , Johns Hopkins University School of Medicine , Baltimore , MD , USA.
Susan H
SH
Eshleman
D000293:Adolescent; D000328:Adult; D001741:Black or African American; D000595:Amino Acid Sequence; D018791:CD4 Lymphocyte Count; D015699:HIV Envelope Protein gp120 / Q000737:chemistry / Q000378:metabolism; D015658:HIV Infections / Q000378:metabolism* / Q000635:transmission / Q000821:virology*; D015497:HIV-1 / Q000502:physiology*; D018451:Homosexuality, Male; D006801:Humans; D008297:Male; D008875:Middle Aged; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000378:metabolism*; D015728:Receptors, HIV / Q000378:metabolism*; D012307:Risk Factors; D019562:Viral Load; D056189:Viral Tropism; D055815:Young Adult
D015699:HIV Envelope Protein gp120; C059762:HIV envelope protein gp120 (305-321); D010446:Peptide Fragments; D019718:Receptors, CXCR4; D015728:Receptors, HIV
Black; CXCR4; Dual-tropic; Dual/mixed; HIV; Men who have sex with men; Tropism