[Proteomic Analysis of Rat Brain Stem with DAI by MALDI-TOF-MS].
OBJECTIVE
To establish a diagnostic model for diffuse axonal injury (DAI) by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). To screen the proteins or peptides associated with DAI for providing the biomarkers with theoretic foundation.


METHODS
Fifteen male Sprague-Dawley rats were randomly divided into DAI group (n = 10) and control group (n = 5). The protein or peptide expression profiles of rat brain stem were detected by MALDI-TOF-MS. ClinProTools 2.2 software was used to find specific peaks, and a diagnostic model was established by the genetic algorithm.


RESULTS
There were significant differences in 61 peaks of DAI group (P < 0.05), 9 peaks were down-regulated and 52 up-regulated. The diagnostic model was established based on 5 different peaks. The specificity and sensitivity of cross validation was 96.14% and 95.98%; while the specificity and sensitivity of blind validation showed was 73.33% and 70.00%, respectively.


CONCLUSIONS
A specific and sensitive diagnostic model of DAI can be established by MALDI-TOF-MS to provide a potential value for determining DAI in forensic practice.
Guan-heng
GH
Ren
Ning-guo
NG
Liu
Yi-jiu
YJ
Chen
Yan
Y
Shi
Dong-hua
DH
Zou
Ping
P
Huang
Zheng-dong
ZD
Li
Ho Yu
HY
Sha
Kai-fei
KF
Deng
D000818:Animals; D015415:Biomarkers; D001933:Brain Stem / Q000378:metabolism*; D020833:Diffuse Axonal Injury / Q000097:blood / Q000175:diagnosis*; D015536:Down-Regulation; D008297:Male; D010455:Peptides / Q000097:blood*; D040901:Proteomics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity; D012984:Software; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D015854:Up-Regulation
D015415:Biomarkers; D010455:Peptides