Application of imaging flow cytometry for characterization of acute inflammation in non-classical animal model systems.
Phagocytes display marked heterogeneity in their capacity to induce and control acute inflammation. This has a significant impact on the effectiveness of antimicrobial immune responses at different tissue sites as well as their predisposition for inflammation-associated pathology. Imaging flow cytometry provides novel opportunities for characterization of these phagocyte populations through high spatial resolution, statistical robustness, and a broad range of quantitative morphometric cell analysis tools. This study highlights an integrative approach that brings together new tools in imaging flow cytometry with conventional methodologies for characterization of phagocyte responses during acute inflammation. We focus on a comparative avian in vivo challenge model to showcase the added depth gained through these novel quantitative multiparametric approaches even in the absence of antibody-based cellular markers. Our characterization of acute inflammation in this model shows significant conservation of phagocytic capacity among avian phagocytes compared to other animal models. However, it also highlights evolutionary divergence with regards to phagocyte inflammation control mechanisms based on the internalization of apoptotic cells.
Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2P5, Canada.
Juan A
JA
More Bayona
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada.
Anbu K
AK
Karuppannan
Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2P5, Canada.
Michael J
MJ
Trites
Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2P5, Canada; Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada. Electronic address: dan.barreda@ualberta.ca.
Daniel R
DR
Barreda
D000208:Acute Disease; D000818:Animals; D000880:Anthraquinones / Q000737:chemistry; D017209:Apoptosis / Q000187:drug effects; D001854:Bone Marrow Cells / Q000187:drug effects / Q000276:immunology / Q000648:ultrastructure; D002645:Chickens; D004396:Coloring Agents / Q000737:chemistry; D005434:Flow Cytometry / Q000379:methods*; D019044:Image Cytometry / Q000379:methods*; D007274:Injections, Intraperitoneal; D007958:Leukocyte Count; D007962:Leukocytes / Q000187:drug effects / Q000276:immunology* / Q000648:ultrastructure; D010537:Peritoneum / Q000187:drug effects / Q000276:immunology / Q000648:ultrastructure; D010538:Peritonitis / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology; D012394:Rosaniline Dyes / Q000737:chemistry; D013194:Staining and Labeling / Q000379:methods*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015054:Zymosan / Q000008:administration & dosage
C520537:1,5-bis((2-(methylamino)ethyl)amino)-4,8-dihydroxyanthracene-9,10-dione; D000880:Anthraquinones; D004396:Coloring Agents; D012394:Rosaniline Dyes; D015054:Zymosan; C035906:Fast Green; D013755:Tetradecanoylphorbol Acetate
Acute inflammation; Chicken leukocytes; Comparative immunology; Evolution; Imaging flow cytometry; Phagocytosis