Functionalized Antimicrobial Composite Thin Films Printing for Stainless Steel Implant Coatings.
In this work we try to address the large interest existing nowadays in the better understanding of the interaction between microbial biofilms and metallic implants. Our aimed was to identify a new preventive strategy to control drug release, biofilm formation and contamination of medical devices with microbes. The transfer and printing of novel bioactive glass-polymer-antibiotic composites by Matrix-Assisted Pulsed Laser Evaporation into uniform thin films onto 316 L stainless steel substrates of the type used in implants are reported. The targets were prepared by freezing in liquid nitrogen mixtures containing polymer and antibiotic reinforced with bioglass powder. The cryogenic targets were submitted to multipulse evaporation by irradiation with an UV KrF* (λ = 248 nm, τFWHM ≤ 25 ns) excimer laser source. The prepared structures were analyzed by infrared spectroscopy, scanning electron microscopy, energy dispersive X-ray spectroscopy and profilometry, before and after immersion in physiological fluids. The bioactivity and the release of the antibiotic have been evaluated. We showed that the incorporated antibiotic underwent a gradually dissolution in physiological fluids thus supporting a high local treatment efficiency. Electrochemical measurements including linear sweep voltammetry and impedance spectroscopy studies were carried out to investigate the corrosion resistance of the coatings in physiological environments. The in vitro biocompatibility assay using the MG63 mammalian cell line revealed that the obtained nanostructured composite films are non-cytotoxic. The antimicrobial effect of the coatings was tested against Staphylococcus aureus and Escherichia coli strains, usually present in implant-associated infections. An anti-biofilm activity was evidenced, stronger against E. coli than the S. aureus strain. The results proved that the applied method allows for the fabrication of implantable biomaterials which shield metal ion release and possess increased biocompatibility and resistance to microbial colonization and biofilm growth.
Faculty of Electrical Engineering and Computer Science, 1 Politehnicii Str., Transilvania University of Brasov, Brasov 500024, Romania. lauraf@unitbv.ro.
Laura
L
Floroian
National Institute for Laser, Plasma and Radiation Physics, P.O. Box MG-36, Magurele, Ilfov RO-77125, Romania. carmen.ristoscu@inflpr.ro.
Carmen
C
Ristoscu
National Institute for Laser, Plasma and Radiation Physics, P.O. Box MG-36, Magurele, Ilfov RO-77125, Romania. natalia.serban@inflpr.ro.
Natalia
N
Mihailescu
National Institute for Laser, Plasma and Radiation Physics, P.O. Box MG-36, Magurele, Ilfov RO-77125, Romania. negut.irina@inflpr.ro.|Faculty of Physics, University of Bucharest, Magurele, Ilfov 077125, Romania. negut.irina@inflpr.ro.
Irina
I
Negut
Faculty of Medicine, 56 N. Balcescu Str., Transilvania University of Brasov, Brasov 500019, Romania. mihaela.badea@unitbv.ro.
Mihaela
M
Badea
Faculty of Electrical Engineering and Computer Science, 1 Politehnicii Str., Transilvania University of Brasov, Brasov 500024, Romania. udoru@unitbv.ro.
Doru
D
Ursutiu
Faculty of Biology, Research Institute of the University of Bucharest-ICUB, University of Bucharest, Spl. Independentei 91-95, Bucharest 050095, Romania. carmen_balotescu@yahoo.com.
Mariana Carmen
MC
Chifiriuc
National Institute for Laser, Plasma and Radiation Physics, P.O. Box MG-36, Magurele, Ilfov RO-77125, Romania. iuliana.iordache@inflpr.ro.
Iuliana
I
Urzica
Faculty of Biology, Research Institute of the University of Bucharest-ICUB, University of Bucharest, Spl. Independentei 91-95, Bucharest 050095, Romania. mdyia1981@gmail.com.
Hussien Mohammed
HM
Dyia
"Stefan S. Nicolau" Institute of Virology, 285 Mihai Bravu Avenue, Bucharest 30304, Romania. cbleotu@yahoo.com.
Coralia
C
Bleotu
National Institute for Laser, Plasma and Radiation Physics, P.O. Box MG-36, Magurele, Ilfov RO-77125, Romania. ion.mihailescu@inflpr.ro.
Ion N
IN
Mihailescu
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000627:therapeutic use; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000627:therapeutic use; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D002516:Ceramics / Q000737:chemistry / Q000627:therapeutic use; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000627:therapeutic use; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D008855:Microscopy, Electron, Scanning; D011327:Printing; D019736:Prostheses and Implants / Q000382:microbiology*; D013193:Stainless Steel / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; C022107:Bioglass; D020099:Coated Materials, Biocompatible; D013193:Stainless Steel
MAPLE thin films; antibiotic release; antimicrobial effect; functional coatings