Water Collective Dynamics in Whole Photosynthetic Green Algae as Affected by Protein Single Mutation.
In the context of the importance of water molecules for protein function/dynamics relationship, the role of water collective dynamics in Chlamydomonas green algae carrying both native and mutated photosynthetic proteins has been investigated by neutron Brillouin scattering spectroscopy. Results show that single point genetic mutation may notably affect collective density fluctuations in hydrating water providing important insight on the transmission of information possibly correlated to biological functionality. In particular, we highlight that the damping factor of the excitations is larger in the native compared to the mutant algae as a signature of a different plasticity and structure of the hydrogen bond network.
CNR Istituto Officina dei Materiali c/o Institut Laue Langevin , 38042 Grenoble, France.|Institut Lumière Matière, Université de Lyon 1 , 69100 Lyon, France.
Daniela
D
Russo
CNR Istituto di Crystallografia 00015 Monterotondo Scalo, 70126 Roma, Italy.
Giuseppina
G
Rea
CNR Istituto di Crystallografia 00015 Monterotondo Scalo, 70126 Roma, Italy.
Maya D
MD
Lambreva
ILL Deuteration Laboratory, Partnership for Structural Biology, 38042 Grenoble, France.|Life Sciences Group, Institut Laue-Langevin , 38000 Grenoble, France.
Michael
M
Haertlein
ILL Deuteration Laboratory, Partnership for Structural Biology, 38042 Grenoble, France.|Life Sciences Group, Institut Laue-Langevin , 38000 Grenoble, France.
Martine
M
Moulin
CNR Istituto Officina dei Materiali c/o Institut Laue Langevin , 38042 Grenoble, France.
Alessio
A
De Francesco
CNR Istituto di Crystallografia 00015 Monterotondo Scalo, 70126 Roma, Italy.
Gaetano
G
Campi
D000460:Chlorophyta / Q000235:genetics* / Q000502:physiology; D006860:Hydrogen Bonding; D008958:Models, Molecular; D009154:Mutation; D033363:Neutron Diffraction; D010788:Photosynthesis / Q000235:genetics*; D013057:Spectrum Analysis; D014867:Water / Q000737:chemistry
D014867:Water