Distinct recirculation potential of CD69+CD103-and CD103+thymic memory CD8+T cells.
Tissue-resident memory T (TRM) cells occupy peripheral and lymphoid tissues where they confer protection against local infection. While epithelial CD8+TRMcells coexpress CD69 and CD103, CD103-memory cells have been described in various organs and are often presumed non-recirculating based on their expression of CD69. We found that both CD69+CD103+and CD69+CD103-memory cells populated the thymus upon transfer of CD8+effector T cells into uninfected recipients. Transcriptionally and phenotypically, CD103+thymic cells resembled non-lymphoid TRMcells, whereas CD69+CD103-cells displayed a profile that was more closely related to recirculating cells. Although CD69 was required for optimal CD103+TRMformation, its expression alone did not identify permanently resident cells, as CD69+CD103-cells disappeared from the thymus following antibody-mediated depletion of recirculating cells. Our findings highlight a distinct migration potential of phenotypically divergent thymic CD8+memory T cells and emphasise the inadequacy of CD69 as a marker of tissue residency.
Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Parkville, VIC, Australia.
Simone L
SL
Park
Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Parkville, VIC, Australia.
Laura K
LK
Mackay
Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, The University of Melbourne, Parkville, VIC, Australia.
Thomas
T
Gebhardt
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology*; D002454:Cell Differentiation / Q000276:immunology; D002465:Cell Movement; D005260:Female; D006801:Humans; D007156:Immunologic Memory; D008213:Lymphocyte Activation; D051379:Mice; D010641:Phenotype; D013950:Thymus Gland / Q000166:cytology*; D014158:Transcription, Genetic
D015703:Antigens, CD