Goose Parvovirus and Circovirus Coinfections in Ornamental Ducks.
Clinical observations and diagnostic procedures carried out to elucidate the cause of high mortality in 2-8-wk-old ornamental ducks (mandarin, wood, falcated, and silver teal ducks) are described. At necropsy, ducklings showed general pallor of skeletal and heart muscles, subcutaneous gelatinous transudates, pericarditis, ascites, and severe edema and hyperemia of lungs. Histopathologic examination revealed that the most important changes were located in the crop, bursa of Fabricius, and lungs with presence of amorphic basic intracytoplasmic inclusions. No bacteria or fungi could be detected from affected organs and ascitic fluid. Viral diagnosis included molecular detection for the presence of goose parvovirus (GPV), circovirus, avian influenza, herpesviruses, paramyxovirus, reovirus, and polyomavirus. Both GPV and circovirus could be detected by real-time PCR and nested broad-spectrum PCR, respectively. Phylogenetically, full-length nucleotide sequence of GPV showed a close similarity ranging from 95.6% to 97.9% with European and Asian pathogenic GPV. On the other hand, the detected circovirus showed nucleotide identity of 90% to 98% with goose circoviruses (GoCVs). This is the first report of GoCVs and GPV in ornamental ducks. The concurrence of GPV and GoCV infections is thought to contribute to the high mortality.
A Center for Infectious Diseases, Institute of Virology, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 29, 04103 Leipzig, Germany.|B Avian and Rabbit Diseases Department, Faculty of Veterinary Medicine, Sadat City University, 22857 Minoufiya, Egypt.
Awad A
AA
Shehata
C Pathology Laboratory NOIVBD, Wintelresedijk 51, 5507 PP Veldhoven, the Netherlands.
Dorrestein M
DM
Gerry
A Center for Infectious Diseases, Institute of Virology, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 29, 04103 Leipzig, Germany.
Kristin
K
Heenemann
A Center for Infectious Diseases, Institute of Virology, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 29, 04103 Leipzig, Germany.
Mohammed Y
MY
Halami
D AviExpert S.P.W.C.P, ul. Gajowa 1, 20-827 Lublin, Poland.
Stanisław
S
Tokarzewski
D AviExpert S.P.W.C.P, ul. Gajowa 1, 20-827 Lublin, Poland.
Peter
P
Wencel
A Center for Infectious Diseases, Institute of Virology, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 29, 04103 Leipzig, Germany.
Thomas W
TW
Vahlenkamp
D000818:Animals; D001715:Bird Diseases / Q000175:diagnosis* / Q000635:transmission / Q000821:virology; D018173:Circoviridae Infections / Q000175:diagnosis / Q000635:transmission / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000235:genetics / Q000302:isolation & purification*; D060085:Coinfection / Q000175:diagnosis / Q000635:transmission / Q000662:veterinary* / Q000821:virology; D000229:Dependovirus / Q000235:genetics / Q000302:isolation & purification*; D004372:Ducks; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000635:transmission / Q000662:veterinary* / Q000821:virology; D057805:Pets; D011044:Poland; D017422:Sequence Analysis, DNA / Q000662:veterinary
coinfection; goose circovirus; goose parvovirus; interspecies transmission; ornamental ducks; phylogenetic tree; sequencing