Variation in copy number of the 28S rDNA of Aspergillus fumigatus measured by droplet digital PCR and analog quantitative real-time PCR.
Droplet digital PCR (ddPCR) after DNA digestion yielded a 28S rDNA copy number of 61 to 86 copies/genome when testing 10 unrelated Aspergillus fumigatus isolates, higher than with quantitative PCR. Unfortunately, ddPCR after DNA digestion did not improve the sensitivity of our PCR assay when testing serum patients with invasive aspergillosis.
Parasitology-Mycology Laboratory, Lariboisière Saint-Louis Fernand Widal Hospitals, Assistance Publique-Hôpitaux de Paris (AP-HP), France; Université Paris-Diderot, Sorbonne Paris Cité, France; Institut Pasteur, Molecular Mycology Unit, CNRS URA3012, National Reference Center of Invasive Mycoses and Antifungals, France.
Alexandre
A
Alanio
Institut Pasteur, Molecular Mycology Unit, CNRS URA3012, National Reference Center of Invasive Mycoses and Antifungals, France.
Aude
A
Sturny-Leclère
Institut Pasteur, Human Genetics and Cognitive Functions Unit, Paris, France.
Marion
M
Benabou
Parasitology-Mycology Laboratory, Lariboisière Saint-Louis Fernand Widal Hospitals, Assistance Publique-Hôpitaux de Paris (AP-HP), France.
Nicolas
N
Guigue
Parasitology-Mycology Laboratory, Lariboisière Saint-Louis Fernand Widal Hospitals, Assistance Publique-Hôpitaux de Paris (AP-HP), France; Université Paris-Diderot, Sorbonne Paris Cité, France; Institut Pasteur, Molecular Mycology Unit, CNRS URA3012, National Reference Center of Invasive Mycoses and Antifungals, France. Electronic address: stephane.bretagne@aphp.fr.
Stéphane
S
Bretagne
D001228:Aspergillosis / Q000175:diagnosis / Q000382:microbiology; D001232:Aspergillus fumigatus / Q000235:genetics*; D056915:DNA Copy Number Variations; D004275:DNA, Ribosomal / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods; D012680:Sensitivity and Specificity
D004275:DNA, Ribosomal; D012339:RNA, Ribosomal, 28S
Aspergillus fumigatus; Copy number variation; Droplet digital PCR; Real-time quantitative PCR; rDNA