Anthelmintic Effect of Bacillus thuringiensis Strains against the Gill Fish Trematode Centrocestus formosanus.
Parasitic agents, such as helminths, are the most important biotic factors affecting aquaculture, and the fluke Centrocestus formosanus is considered to be highly pathogenic in various fish species. There have been efforts to control this parasite with chemical helminthicides, but these efforts have had unsuccessful results. We evaluated the anthelmintic effect of 37 strains of Bacillus thuringiensis against C. formosanus metacercariae in vitro using two concentrations of total protein, and only six strains produced high mortality. The virulence (CL50) on matacercariae of three strains was obtained: the GP308, GP526, and ME1 strains exhibited a LC50 of 146.2 μg/mL, 289.2 μg/mL, and 1721.9 μg/mL, respectively. Additionally, these six B. thuringiensis strains were evaluated against the cercariae of C. formosanus; the LC50 obtained from the GP526 strain with solubilized protein was 83.8 μg/mL, and it could be considered as an alternative control of the metacercariae and cercariae of this parasite in the productivity systems of ornamental fishes.
Facultad de Ciencias Agropecuarias, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001, Colonia Chamilpa, 62209 Cuernavaca, MOR, Mexico.
Luis Javier
LJ
Mendoza-Estrada
Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001, Colonia Chamilpa, 62209 Cuernavaca, MOR, Mexico.
Víctor Manuel
VM
Hernández-Velázquez
Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Campus Morelos, Avenida Universidad 2001, 62210 Cuernavaca, MOR, Mexico.
Iván
I
Arenas-Sosa
Facultad de Ciencias Agropecuarias, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001, Colonia Chamilpa, 62209 Cuernavaca, MOR, Mexico.
Fernando Iván
FI
Flores-Pérez
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, AP 70228, 04510 México, DF, Mexico.
Jorge
J
Morales-Montor
Centro de Investigaciones Biológicas, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001, Colonia Chamilpa, 62209 Cuernavaca, MOR, Mexico.
Guadalupe
G
Peña-Chora
D000818:Animals; D000871:Anthelmintics / Q000627:therapeutic use; D001413:Bacillus thuringiensis / Q000502:physiology*; D001426:Bacterial Proteins / Q000737:chemistry; D001681:Biological Assay; D061046:Biological Control Agents; D005393:Fish Diseases / Q000469:parasitology* / Q000628:therapy*; D005399:Fishes / Q000469:parasitology*; D012908:Snails / Q000382:microbiology; D013171:Spores, Bacterial / Q000502:physiology; D016896:Treatment Outcome; D014200:Trematoda / Q000382:microbiology*; D014201:Trematode Infections / Q000628:therapy*; D014774:Virulence
D000871:Anthelmintics; D001426:Bacterial Proteins; D061046:Biological Control Agents