Beneficial roles of melanoma cell adhesion molecule in spinal cord transection recovery in adult zebrafish.
Melanoma cell adhesion molecule (MCAM) is a multifunctional protein involved in miscellaneous processes, including development and tumor angiogenesis. Here, spinal cord transection in adult zebrafish was used to investigate the effects of MCAM on spinal cord injury (SCI) and subsequent recovery. Expression of MCAM mRNA increased and co-localized with motoneurons in the spinal cord after SCI. With MCAM morpholino treatment, inhibition of MCAM retarded both axon regrowth and locomotor recovery in the spinal cord injured zebrafish. Furthermore, MCAM mRNA expression was also observed in fli1a:EGFP transgenic zebrafish, which specifically show labeled blood vessels. Inhibition of MCAM down-regulated the expression of angiogenesis-related factors, such as VEGFR-2, p-p38 and p-AKT, and the inflammatory factors TNF-α, IL-1β and IL-8. Taken together, these data suggest that MCAM may have a beneficial role in the recovery from SCI, via the promotion of neurogenesis and angiogenesis. In the context of adult zebrafish spinal cord injury, we proved that Melanoma cell adhesion molecule (MCAM) is beneficial to the recovery, possibly via mechanisms of angiogenensis and inflammation. MCAM promotes angiogenesis by adjusting VEGFR-2, p-p38 and p-AKT. MCAM affects inflammatory factors such as TNF-α, IL-1β and IL-8. Our results extend the beneficial role of MCAM in the regeneration of central nervous system.
Jiangnan University Medical School, Wuxi, China.|Center for Neuroscience, Shantou University Medical College, Shantou, China.
Chun-Jie
CJ
Liu
Affiliated Hospital of Jining Medical University, Jining, China.
Lin
L
Xie
Jiangnan University Medical School, Wuxi, China.
Chun
C
Cui
Jiangnan University Medical School, Wuxi, China.
Min
M
Chu
Jiangnan University Medical School, Wuxi, China.|Center for Neuroscience, Shantou University Medical College, Shantou, China.
Hou-De
HD
Zhao
Jiangnan University Medical School, Wuxi, China.
Li
L
Yao
Jiangnan University Medical School, Wuxi, China.
Yu-Hong
YH
Li
Center for Neuroscience, Shantou University Medical College, Shantou, China.
Melitta
M
Schachner
Jiangnan University Medical School, Wuxi, China. shenyanqin@jiangnan.edu.cn.|Center for Neuroscience, Shantou University Medical College, Shantou, China. shenyanqin@jiangnan.edu.cn.
Yan-Qin
YQ
Shen
D043924:Angiogenesis Modulating Agents / Q000494:pharmacology; D000818:Animals; D030801:Animals, Genetically Modified; D001369:Axons / Q000187:drug effects / Q000473:pathology; D001808:Blood Vessels / Q000378:metabolism; D051929:CD146 Antigen / Q000235:genetics* / Q000378:metabolism; D002452:Cell Count; D018836:Inflammation Mediators / Q000502:physiology; D008124:Locomotion; D009025:Morpholines / Q000494:pharmacology; D009046:Motor Neurons / Q000187:drug effects; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020127:Recovery of Function; D013119:Spinal Cord Injuries / Q000235:genetics* / Q000473:pathology*; D013550:Swimming; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism*
D043924:Angiogenesis Modulating Agents; D051929:CD146 Antigen; D018836:Inflammation Mediators; D009025:Morpholines; D012333:RNA, Messenger; D029961:Zebrafish Proteins; C000620397:mcamb protein, zebrafish

MCAM
; blood vessels; inflammation; regeneration; spinal cord injury; zebrafish