Determination of natural phenols in olive fruits by chitosan assisted matrix solid-phase dispersion microextraction and ultrahigh performance liquid chromatography with quadrupole time-of-flight tandem mass spectrometry.
A simple, efficient and low-cost method based on matrix solid-phase dispersion (MSPD) microextraction and ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UHPLC-Q-TOF/MS) was developed for the determination of seven main natural phenols (gallic acid, hydroxytyrosol, methyl gallate, luteolin 7-O-β-d-glucoside, rutin, ellagic acid and oleuropein) and other eleven compounds in olive fruits. The experimental conditions for the MSPD extraction, including the type of adsorbent, the amount of dispersing sorbent, the grinding time, and the type of elution solvent were investigated and optimized. The optimized parameters were determined to be that middle-molecular-weight chitosan was used as adsorbent, the amount of middle-molecular-weight chitosan was selected to be 25mg, the grinding time was chosen to be 60s, and methanol: water (6:4, v:v) was used as elution solvent. Compared with reported methods, the proposed method was more simple, rapid, and efficient. Moreover, this method required less extraction time and less amount of sample and solvent. The method showed good linearity (r(2)≥0.9909) for the seven analytes, with the limits of detection in the range of 69.6-358.4ng/g. And recoveries were above 80.06%. The methodology was successfully applied to the extraction and determination of seven phenolic compounds in olive fruits（Canarii fructus）.
College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.
Li-Qing
LQ
Peng
Institute of Matetia Medica, Zhejiang Acdemy of Medical Sciences, Hangzhou 310013, China.
Qin
Q
Li
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China. Electronic address: tcmcyx@126.com.
Yan-Xu
YX
Chang
Department of Surgery, University of Michigan Medical Center, Ann Arbor, MI 48109, United States.
Mingrui
M
An
Department of Surgery, University of Michigan Medical Center, Ann Arbor, MI 48109, United States.
Rui
R
Yang
Department of Surgery, University of Michigan Medical Center, Ann Arbor, MI 48109, United States.
Zhijing
Z
Tan
Periodontics and Oral Medicine, School of Dentistry, University of Michigan, Ann Arbor, MI 48109, United States.
Jie
J
Hao
College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China. Electronic address: caojun91@163.com.
Jun
J
Cao
College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.
Jing-Jing
JJ
Xu
College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.
Shuai-Shuai
SS
Hu
D029403:Burseraceae / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005638:Fruit / Q000737:chemistry; D000432:Methanol; D010636:Phenols / Q000032:analysis*; D052617:Solid Phase Microextraction; D012997:Solvents; D053719:Tandem Mass Spectrometry
D010636:Phenols; D012997:Solvents; D048271:Chitosan; D000432:Methanol
Matrix solid-phase dispersion; Natural phenols; Olive fruits; Quadrupole time-of-flight tandem mass spectrometry; Ultrahigh-performance liquid chromatography