Serine 231 and 257 of Agamous-like 15 are phosphorylated in floral receptacles.
The large dynamic range of gene expression changes accompanying floral organ abscission can be explained by a molecular positive feedback loop that regulates the process. In short, a mitogen-activated protein kinase (MAPK) cascade, positioned genetically downstream from the abscission receptor HAESA (HAE), phosphorylates the transcription factor, AGAMOUS-like 15 (AGL15), allowing HAE to be expressed. However, it is unknown which residues of AGL15 are phosphorylated and precisely how phosphorylation alters AGL15 function. Here we report that serine 231 and 257 of AGL15 are phosphorylated in floral receptacles. Effects of phosphorylation on AGL15 are discussed.
a Division of Biological Sciences and Interdisciplinary Plant Group, University of Missouri , Columbia , MO , USA.
Osric Rahul
OR
Patharkar
a Division of Biological Sciences and Interdisciplinary Plant Group, University of Missouri , Columbia , MO , USA.
Terra A
TA
Macken
a Division of Biological Sciences and Interdisciplinary Plant Group, University of Missouri , Columbia , MO , USA.
John C
JC
Walker
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D035264:Flowers / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D026161:MADS Domain Proteins / Q000378:metabolism*; D010766:Phosphorylation; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D012694:Serine / Q000378:metabolism
C522473:AGL15 protein, Arabidopsis; D029681:Arabidopsis Proteins; D026161:MADS Domain Proteins; D012694:Serine
Abscission; MAPK cascade; phosphorylation; signal transduction; transcription factor