Complexity and Dynamics of the Winemaking Bacterial Communities in Berries, Musts, and Wines from Apulian Grape Cultivars through Time and Space.
Currently, there is very little information available regarding the microbiome associated with the wine production chain. Here, we used an amplicon sequencing approach based on high-throughput sequencing (HTS) to obtain a comprehensive assessment of the bacterial community associated with the production of three Apulian red wines, from grape to final product. The relationships among grape variety, the microbial community, and fermentation was investigated. Moreover, the winery microbiota was evaluated compared to the autochthonous species in vineyards that persist until the end of the winemaking process. The analysis highlighted the remarkable dynamics within the microbial communities during fermentation. A common microbial core shared among the examined wine varieties was observed, and the unique taxonomic signature of each wine appellation was revealed. New species belonging to the genus Halomonas were also reported. This study demonstrates the potential of this metagenomic approach, supported by optimized protocols, for identifying the biodiversity of the wine supply chain. The developed experimental pipeline offers new prospects for other research fields in which a comprehensive view of microbial community complexity and dynamics is desirable.
Institute of Biomembranes and Bioenergetics, CNR, Bari, Italy.
Marinella
M
Marzano
Institute of Biomembranes and Bioenergetics, CNR, Bari, Italy.
Bruno
B
Fosso
Institute of Biomembranes and Bioenergetics, CNR, Bari, Italy.
Caterina
C
Manzari
Institute of Sciences of Food Production, CNR, Operative Unit of Lecce, Lecce (Le), Italy.
Francesco
F
Grieco
Department of Biosciences, Biotechnology and Biopharmaceutics, University of Bari "A. Moro" Bari, Italy.
Marianna
M
Intranuovo
Institute of Sciences of Food Production, CNR, Bari, Italy.
Giuseppe
G
Cozzi
Institute of Sciences of Food Production, CNR, Bari, Italy.
Giuseppina
G
Mulè
GBS BAO Advanced Analytics Services and MBLab, IBM, Bari, Italy.
Gaetano
G
Scioscia
Algorithms, Bioinformatics, Complexity and Formal Methods Research Group, Polytechnic University of Catalonia, Barcelona, Spain.
Gabriel
G
Valiente
Institute for Biomedical Technologies, CNR, Bari, Italy.
Apollonia
A
Tullo
Institute for Biomedical Technologies, CNR, Bari, Italy.
Elisabetta
E
Sbisà
Institute of Biomembranes and Bioenergetics, CNR, Bari, Italy.|Department of Biosciences, Biotechnology and Biopharmaceutics, University of Bari "A. Moro" Bari, Italy.
Graziano
G
Pesole
Institute of Biomembranes and Bioenergetics, CNR, Bari, Italy.
Monica
M
Santamaria
D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D005285:Fermentation; D005638:Fruit / Q000382:microbiology; D005658:Fungi / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D057166:High-Throughput Screening Assays; D056186:Metagenomics; D064307:Microbiota; D027843:Vitis / Q000382:microbiology*; D014920:Wine / Q000382:microbiology*