Hepato-protective effects and chemical constituents of a bioactive fraction of the traditional compound medicine-Gurigumu-7.
BACKGROUND
Gurigumu-7 is an important traditional Mongolian medicine frequently used for liver diseases. However, the pharmacological effects and the bioactive constituents are not well understood.


METHODS
This research was to use CCl4-induced liver damage in mice to investigate the hepatoprotective effects of Gurigumu-7 and the methanol eluted fraction from a DIAION column of an extract of Gurigumu-7 (MF). The chemical constituents of MF were analyzed by UPLC-MS.


RESULTS
Pretreated orally with MF (66, 132 and 264 mg/kg) once a day for 4 days dose-dependently suppressed CCl4-induced mice liver histopathological changes and serum aminotransferase activities (alanine transaminase: 1144.0 ± 787.2 v.s. 2461.8 ± 1072.7 U/L, p < 0.05; aspartate transaminase: 1173 ± 785.3 v.s. 2506.6 ± 1140.7 U/L, p < 0.01). MF treated group demonstrated increased levels of SOD (108.19 ± 30.32 v.s. 75.75 ± 5.37 U/mg protein, p < 0.01) but decreased levels of malonyldialdehyde (7.68 ± 1.95 v.s. 44.32 ± 16.68 nmol/mg protein, p < 0.01) compared to the CCl4 control group. More than 30 chemical constituents were quantified, and MF was found to be rich in ellagic acid (297.97 mg/g), luteolin and its glucosides (35.10 mg/g), apigenin and its glucosides (>30 mg/g), ursolic acid (14.91 mg/g), bidenoside C (8.75 mg/g), and proanthocyanidins (15.64 mg/g in proanthocyanidin A2 equivalent).


CONCLUSIONS
The methanol eluted fraction (MF) from a DIAION column of an extract of the Mongolian medicine-Gurigumu-7 was found to be more hepatoprotective than Gurigumu-7. The results suggested that MF is a promising bioactive fraction for the development of new hepatoprotective medicine with better formulation and quality control properties.
Collage of Life Science, Inner Mongolia University, Huhhot, Inner Mongolia, China.
Haiyan
H
Xu
Collage of Life Science, Inner Mongolia University, Huhhot, Inner Mongolia, China.
Qiong
Q
Ma
Collage of Life Science, Inner Mongolia University, Huhhot, Inner Mongolia, China.
Jiannan
J
Ma
Collage of Life Science, Inner Mongolia University, Huhhot, Inner Mongolia, China.
Zhigang
Z
Wu
Collage of Life Science, Inner Mongolia University, Huhhot, Inner Mongolia, China.
Yali
Y
Wang
Collage of Life Science, Inner Mongolia University, Huhhot, Inner Mongolia, China. cmma@imu.edu.cn.
Chaomei
C
Ma
D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D002251:Carbon Tetrachloride; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008986:Mongolia; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D010946:Plants, Medicinal; D020011:Protective Agents / Q000737:chemistry / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D000637:Transaminases / Q000097:blood
D000975:Antioxidants; C000613392:Gurigumu-7; D010936:Plant Extracts; D020011:Protective Agents; D008315:Malondialdehyde; D002251:Carbon Tetrachloride; D013482:Superoxide Dismutase; D000637:Transaminases
Bioactive constituents; Bioactive fraction; Gurigumu-7; Hepatoprotective effect