Protective effect of Cordyceps militaris extract against bisphenol A induced reproductive damage.
This study aimed to investigate the protective effects of Cordyceps militaris (C. militaris) against reproductive damage induced by bisphenol A (BPA). Rats were administrated 200 mg/kg BPA for 4 weeks and treated with C. militaris (200, 400, and 800 mg/kg body weight/day). By the end of the fourth week, the level of oxidative damage, sperm parameters, hormone levels, and histopathological changes were examined. In the group that only received BPA, there was a signiﬁcant decrease in body weight compared with the normal control (NC) group. C. militaris signiﬁcantly alleviated the BPA-induced reproductive damage by increasing testicular superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), and glutathione (GSH); as well as by reducing serum malondialdehyde (MDA). C. militaris not only obviously enhanced the levels of serum LH and T, but it also improved the sperm count and motility compared to the BPA-treated group. These results suggest that C. militaris could be used as a potential natural substance for preventing BPA induced reproductive damage. Abbreviations BPA: bisphenol A; SOD: superoxide dismutase; GSH: glutathione; GSH-PX: glutathione peroxidase; MDA: malondialdehyde; ROS: reactive oxygen species; T: testosterone; LH: luteinizing hormone; FSH: follicle-stimulating hormone; UPLC: ultra performance liquid chromatography; RIA: radioimmunoassay; q
quantitative real time PCR; NC: normal control group; BPA: 200 mg/kg BPA administered group; H: 800 mg/kg C. militaris extract administered group; LB, MB, and HB: 200 mg/kg BPA + 200 mg/kg, 400 mg/kg, and 800 mg/kg C. militaris administered group, respectively; VeB: 200 mg/kg BPA + 300 mg/kg Vitamin E administered group; Star: steroidogenic acute regulatory protein; 3β-HSD: 3beta-hydroxyl-delta-5-steroid dehydrogenase; CYP11A1: cytochrome P 450 family 11 subfamily A member 1; CYP17A1: cytochrome P 450 family 17 subfamily A member 1.
a Chinese-German Joint Laboratory for Natural Product Research, Qinling-Bashan Mountains Bioresources Comprehensive Development C.I.C., College of Biological Science and Engineering , Shaanxi University of Technology , Hanzhong , Shaanxi , China.|b College of Veterinary Medicine , Northwest A&F University , Yangling , Shaanxi , China.
Jian
J
Wang
a Chinese-German Joint Laboratory for Natural Product Research, Qinling-Bashan Mountains Bioresources Comprehensive Development C.I.C., College of Biological Science and Engineering , Shaanxi University of Technology , Hanzhong , Shaanxi , China.
Chen
C
Chen
b College of Veterinary Medicine , Northwest A&F University , Yangling , Shaanxi , China.
Zhihui
Z
Jiang
b College of Veterinary Medicine , Northwest A&F University , Yangling , Shaanxi , China.
Meng
M
Wang
a Chinese-German Joint Laboratory for Natural Product Research, Qinling-Bashan Mountains Bioresources Comprehensive Development C.I.C., College of Biological Science and Engineering , Shaanxi University of Technology , Hanzhong , Shaanxi , China.
Hai
H
Jiang
a Chinese-German Joint Laboratory for Natural Product Research, Qinling-Bashan Mountains Bioresources Comprehensive Development C.I.C., College of Biological Science and Engineering , Shaanxi University of Technology , Hanzhong , Shaanxi , China.|b College of Veterinary Medicine , Northwest A&F University , Yangling , Shaanxi , China.
Xiaoying
X
Zhang
D000818:Animals; D001559:Benzhydryl Compounds / Q000037:antagonists & inhibitors* / Q000633:toxicity; D032661:Cordyceps / Q000737:chemistry*; D003839:Deoxyadenosines / Q000302:isolation & purification; D006728:Hormones / Q000097:blood; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress; D010636:Phenols / Q000037:antagonists & inhibitors* / Q000633:toxicity; D011134:Polysaccharides / Q000302:isolation & purification; D020011:Protective Agents / Q000302:isolation & purification* / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012098:Reproduction / Q000187:drug effects; D013091:Spermatogenesis / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects; D013737:Testis / Q000187:drug effects*; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D003839:Deoxyadenosines; D006728:Hormones; D010636:Phenols; D011134:Polysaccharides; D020011:Protective Agents; C058120:cordycepin; C006780:bisphenol A; D000098826:Bisphenol A Compounds
Bisphenol A; Cordyceps militaris; reproductive damage; testis