Effects of continuous wave and fractionated diode laser on human fibroblast cancer and dermal normal cells by zinc phthalocyanine in photodynamic therapy: A comparative study.
In this experimental study, cancer and normal cells behavior during an in vitro photodynamic therapy (PDT) under exposure of continuous wave (CW) and fractionated mode of laser with different irradiation power and time intervals was compared and investigated. At the first, human fibroblast cancer cell line (SW 872) and human dermal normal (HFFF2) cell line were incubated with different concentrations of zinc phthalocyanine (ZnPc), as a PDT drug. The cells, then, were irradiated with a 675nm diode laser and the cell viability was evaluated using MTT assay. Under optimized conditions, the viability of the cancer cells was eventually reduced to 3.23% and 13.17%, and that of normal cells was decreased to 20.83% and 36.23% using CW and fractionated diode lasers, respectively. In general, the ratio of ZnPc LD50 values for the normal cells to the cancer cells with CW laser was much higher than that of the fractionated laser. Subsequently, cancer cells in comparison with normal ones were found to be more sensitive toward the photodynamic damage induced by ZnPc. In addition, treatment with CW laser was found to be more effective against the cancer cells with a lower toxicity to the normal cells compared with the fractionated diode laser.
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Faculty of Physics, Iran University of Science and Technology, Tehran, Iran.
Farzaneh
F
Navaeipour
Faculty of Physics, Iran University of Science and Technology, Tehran, Iran; Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran.
Hadi
H
Afsharan
Research Institute for Applied Physics and Astronomy, University of Tabriz, Tabriz, Iran.
Habib
H
Tajalli
Faculty of Physics, Iran University of Science and Technology, Tehran, Iran.
Mahmood
M
Mollabashi
Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
Farideh
F
Ranjbari
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Azadeh
A
Montaseri
Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran; Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: rashidi@tbzmed.ac.ir.
Mohammad-Reza
MR
Rashidi
D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D007211:Indoles / Q000737:chemistry / Q000627:therapeutic use / Q000633:toxicity*; D054833:Isoindoles; D054023:Lasers, Semiconductor; D009369:Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D009942:Organometallic Compounds / Q000737:chemistry / Q000627:therapeutic use / Q000633:toxicity*; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000737:chemistry / Q000627:therapeutic use / Q000633:toxicity*; D017967:Zinc Compounds
D007211:Indoles; D054833:Isoindoles; D009942:Organometallic Compounds; D017319:Photosensitizing Agents; D017967:Zinc Compounds; C052159:Zn(II)-phthalocyanine
Continuous wave laser; Fractionated laser; Human dermal normal cells; Human fibroblast cancer cells; Photodynamic therapy; Zinc phthalocyanine