Applications of the solvation parameter model in reversed-phase liquid chromatography.
The solvation parameter model is widely used to provide insight into the retention mechanism in reversed-phase liquid chromatography, for column characterization, and in the development of surrogate chromatographic models for biopartitioning processes. The properties of the separation system are described by five system constants representing all possible intermolecular interactions for neutral molecules. The general model can be extended to include ions and enantiomers by adding new descriptors to encode the specific properties of these compounds. System maps provide a comprehensive overview of the separation system as a function of mobile phase composition and/or temperature for method development. The solvation parameter model has been applied to gradient elution separations but here theory and practice suggest a cautious approach since the interpretation of system and compound properties derived from its use are approximate. A growing application of the solvation parameter model in reversed-phase liquid chromatography is the screening of surrogate chromatographic systems for estimating biopartitioning properties. Throughout the discussion of the above topics success as well as known and likely deficiencies of the solvation parameter model are described with an emphasis on the role of the heterogeneous properties of the interphase region on the interpretation and understanding of the general retention mechanism in reversed-phase liquid chromatography for porous chemically bonded sorbents.
Department of Chemistry, Wayne State University, Detroit, MI 48202, USA. Electronic address: cfp@chem.wayne.edu.
Colin F
CF
Poole
Department of Chemistry, Wayne State University, Detroit, MI 48202, USA.
Nicole
N
Lenca
D056148:Chromatography, Reverse-Phase / Q000295:instrumentation; D008567:Membranes, Artificial; D008956:Models, Chemical; D000442:Octanols / Q000737:chemistry; D011312:Pressure; D012997:Solvents / Q000737:chemistry*; D055672:Static Electricity; D013696:Temperature; D014867:Water / Q000737:chemistry
D008567:Membranes, Artificial; D000442:Octanols; D012997:Solvents; D014867:Water
Chromatographic models for biopartitioning; Gradient elution; Interphase model; Retention; Reversed-phase liquid chromatography; Reviews; Solvation parameter model; System maps