[Basic Evaluation of Microsemi LC-767CRP as POCT Model for Simultaneous Measurement of CBC and CRP].
Microsemi LC-767CRP (LC-767, Horiba, Ltd.) is capable of simultaneous measuring of complete blood count (CBC) including 3-part differentials (3-part Diff.) of white blood cells (WBC) and C-reactive protein (CRP) in 4 minutes. Data obtained using LC-767 were intra-assay-reproducible (n = 10, CV = 0.6-4.0% for CBC, 0.6-2.5% for 3-part Diff. and 2.8-7.7% for CRP). They also showed the good linearity, no definite carry-over and the excellent correlations with routine instruments in our institution. Concerning CRP, the minimal detectable concentration revealed < 0.1 mg/dL, and prozone was observed in the sample containing > 30 mg/dL of CRP. LC-767 showed better correlation with a routine instrument in monocyte percentage than LC-667, probably due to modification of the hemolysis solution ratio and diluent temperature. In conclusion, LC-767 provided accurate CBC and CRP results, and showed improvement in CRP linearity and monocyte percentage compared with LC-667. LC-767, which is equipped with a bar-code reader with easy accessibility to electronic medical record, is suitable as the next-generation point of care testing model in the era of information and network-oriented medicine.
Tohru
T
Inaba
Motohide
M
Ikeda
Kensuke
K
Saitoh
Soichi
S
Yuasa
Naoko
N
Mishima
Katsuaki
K
Ogura
Narihiro
N
Oku
Mai
M
Kodama
Yumiko
Y
Fujitomo
Masaki
M
Nakanishi
Naohisa
N
Fujita
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000097:blood; D002097:C-Reactive Protein / Q000032:analysis*; D005260:Female; D006461:Hemolysis; D006801:Humans; D007958:Leukocyte Count / Q000295:instrumentation* / Q000379:methods; D007962:Leukocytes / Q000145:classification; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D012996:Solutions; D013696:Temperature; D055815:Young Adult
D015415:Biomarkers; D012996:Solutions; D002097:C-Reactive Protein