Differential integrative omic analysis for mechanism insights and biomarker discovery of abnormal Savda syndrome and its unique Munziq prescription.
Research has shown that many cancers have acommon pathophysiological origin and often present with similar symptoms. In terms of Traditional Uighur Medicine (TUM) Hilit (body fluid) theory, abnormal Savda syndrome (ASS) formed by abnormal Hilit is the common phenotype of complex diseases and in particular tumours. Abnormal Savda Munziq (ASMq), one representative of TUM, has been effective in the treatment of cancer since ancient times. Despite the physiopathology of ASS, the relationship between causative factors and the molecular mechanism of ASMq are not fully understood. The current study expanded upon earlier work by integrating traditional diagnostic approaches with others utilizing systems biology technology for the analysis of proteomic (iTRAQ) and metabolomic ((1)H-NMR) profiles of Uighur Medicine target organ lesion (liver) tumours. The candidate proteins were analyzed by enrichment analysis of the biological process and biomarker filters. Subsequently, 3Omics web-based tools were used to determine the relationships between proteins and appropriate metabolites. ELISA assay and IHC methods were used to verify the proteomic result; the protein von Willebrand factor (vWF) may be the "therapeutic window" of ASMq and biomarkers of ASS. This study is likely to be of great significance for the standardization and modernization of TUM.
Shenzhen Hospital of Southern Medical University, Shenzhen 518110, PR China.|Xinjiang Key Laboratory of Molecular Biology and Endemic Diseases, Xinjiang Medical University, Urumqi 830011,PR China.
Xia
X
Guo
Department of Biochemistry and Molecular Biology, Xinjiang Medical University, Urumqi 830011, PR China.
Iskandar
I
Bakri
Department of Biochemistry and Molecular Biology, Xinjiang Medical University, Urumqi 830011, PR China.
Abulizi
A
Abudula
Faculty of Traditional Uyghur Medicine, Xinjiang Medical University, Urumqi 830011, PR China.
Kalbinur
K
Arken
Faculty of Traditional Uyghur Medicine, Xinjiang Medical University, Urumqi 830011, PR China.
Mahmut
M
Mijit
Central Laboratory, Xinjiang Medical University, Urumqi 830011, PR China.
Batur
B
Mamtimin
Central Laboratory, Xinjiang Medical University, Urumqi 830011, PR China.
Halmurat
H
Upur
D000818:Animals; D014408:Biomarkers, Tumor / Q000097:blood*; D001826:Body Fluids / Q000378:metabolism; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy*; D008297:Male; D056785:Medicine, Mongolian Traditional; D055432:Metabolomics; D028321:Plant Preparations / Q000627:therapeutic use*; D020543:Proteome; D040901:Proteomics; D051381:Rats; D012015:Reference Standards; D013577:Syndrome; D014841:von Willebrand Factor / Q000378:metabolism*
D014408:Biomarkers, Tumor; D028321:Plant Preparations; D020543:Proteome; D014841:von Willebrand Factor