INSECTICIDE RESISTANCE IN THE GROUND SPIDER, Pardosa sumatrana (THORELL, 1890; ARANEAE: LYCOSIDAE).
Elevated levels of insecticides detoxifying enzymes, such as esterases, glutathione S-transferases (GSTs), and cytochrome P-450 monooxygenases, act in the resistance mechanisms in insects. In the present study, levels of these enzymes in the insecticide-resistant ground spider Pardosa sumatrana (Thorell, 1890) were compared with a susceptible population (control) of the same species. Standard protocols were used for biochemical estimation of enzymes. The results showed significantly higher levels of nonspecific esterases and monooxygenases in resistant spiders compared to controls. The activity of GSTs was lower in the resistant spiders. Elevated levels of nonspecific esterases and monooxygenases suggest their role in metabolic resistance in P. sumatrana. The reduced levels of total protein contents revealed its possible consumption to meet energy demands.
Department of Zoology, GC University, Lahore, Pakistan.
Hafiz Muhammad
HM
Tahir
Department of Zoology, University of Sargodha, Sargodha, Pakistan.
Farva
F
Khizar
Department of Zoology, University of Sargodha, Sargodha, Pakistan.
Sajida
S
Naseem
Department of Zoology, University of Sargodha, Sargodha, Pakistan.
Rabia
R
Yaqoob
Department of Zoology, GC University, Faisalabad, Pakistan.
Khizar
K
Samiullah
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D004950:Esterases / Q000235:genetics / Q000378:metabolism; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D007093:Imidazoles / Q000633:toxicity*; D008658:Inactivation, Metabolic; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007305:Insecticide Resistance; D007306:Insecticides / Q000633:toxicity*; D000073943:Neonicotinoids; D009574:Nitro Compounds / Q000633:toxicity*; D010154:Pakistan; D011722:Pyrethrins / Q000633:toxicity*; D013112:Spiders / Q000187:drug effects*
D007093:Imidazoles; D019476:Insect Proteins; D007306:Insecticides; D000073943:Neonicotinoids; D009574:Nitro Compounds; D011722:Pyrethrins; C082359:imidacloprid; C099952:bifenthrin; D003577:Cytochrome P-450 Enzyme System; D005982:Glutathione Transferase; D004950:Esterases
metabolic enzymes; pest management; resistance; spiders