The Validation Study of Neurofilament Heavy Chain and 8-hydroxy-2'-deoxyguanosine as Plasma Biomarkers of Clinical/Paraclinical Activity in First and Relapsing-Remitting Demyelination Acute Attacks.
Although current evidence mainly suggests immunopathogenesis of demyelination and neurodegeneration in multiple sclerosis (MS), there are results which document the importance of other factors, such as oxidative stress and its mediated injuries. The oxidative stress intensity in axonal damage during acute demyelination is little known. We performed this study as a cross-sectional biomarker validation study in order to evaluate the parameters of axonal damage (phosphorylated neurofilaments heavy chain (pNF-H)) and oxidative stress (8-hydroxy-2'-deoxyguanosine (8-OHdG)) in plasma of patients with initial and relapsing-remitting demyelination attacks, defined as clinically isolated syndrome (CIS) and relapsing-remitting multiple sclerosis (RRMS); and the correlations between these parameters and biological (index of blood brain barrier (BBB) permeability), clinical (index of disease progression), and radiological (T1-Gd-enhancing lesion volume) activities of disease. Both parameters were increased in CIS and RRMS compared to control subjects (p < 0.05). The positive correlations were observed between 8-OHdG values and index of BBB permeability, clinical severity of disease, and demyelinated brain lesion volume, in CIS group (r > 0.50; p < 0.05). Similar correlations were obtained between pNF-H values and the above parameters, as well as the index of disease progression, in RRMS group (r > 0.30; p < 0.05). There was a significant correlation between values of 8-OHdG and pNF-H only in CIS group, r = 0.52, p < 0.05. While the plasma values of 8-OHdG reflect the degree of acute demyelination in CIS, pNF-H values reflect that in RRMS. The obtained results must be reevaluated in similar prospective studies related to their prognostic values.
Clinic of Neurology, Clinical Center Nis, Bul. Dr Zorana Djindjica 48, 18000, Nis, Serbia. srljub@gmail.com.|Faculty of Medicine, University of Nis, Bul. Dr Zorana Djindjica 81, 18000, Nis, Serbia. srljub@gmail.com.
S
S
Ljubisavljevic
Faculty of Medicine, University of Nis, Bul. Dr Zorana Djindjica 81, 18000, Nis, Serbia.
I
I
Stojanovic
Faculty of Medicine, University of Nis, Bul. Dr Zorana Djindjica 81, 18000, Nis, Serbia.
J
J
Basic
Faculty of Medicine, University of Nis, Bul. Dr Zorana Djindjica 81, 18000, Nis, Serbia.
D A
DA
Pavlovic
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000097:blood; D001812:Blood-Brain Barrier / Q000378:metabolism; D001921:Brain / Q000000981:diagnostic imaging; D002199:Capillary Permeability; D003430:Cross-Sectional Studies; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000097:blood; D004185:Disability Evaluation; D018450:Disease Progression; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008279:Magnetic Resonance Imaging; D008297:Male; D008875:Middle Aged; D020529:Multiple Sclerosis, Relapsing-Remitting / Q000097:blood* / Q000000981:diagnostic imaging; D016900:Neurofilament Proteins / Q000097:blood*; D012720:Severity of Illness Index; D055815:Young Adult
D015415:Biomarkers; D016900:Neurofilament Proteins; C051774:neurofilament protein H; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine
8-hydroxy-2′-deoxyguanosine; Demyelination; Neurofilament heavy chain