Dual rapid lateral flow immunoassay fingerstick wholeblood testing for syphilis and HIV infections is acceptable and accurate, Port-au-Prince, Haiti.
Dual rapid tests for HIV and syphilis infections allow for detection of HIV infection and syphilis at the point-of-care. Those tests have been evaluated in laboratory settings and show excellent performance but have not been evaluated in the field. We evaluated the field performance of the SD BIOLINE HIV/Syphilis Duo test in Port-au-Prince, Haiti using whole blood fingerprick specimens.



GHESKIO (Haitian Study Group for Kaposi's Sarcoma and Opportunistic Infections) clinic attendees 18 years of age or older were invited to participate. Venipuncture blood specimens were used for reference testing with standard commercially available tests for HIV and syphilis in Haiti. The sensitivity and specificity of the Duo test compared to the reference standard were calculated. The exact binomial method was used to determine 95 % confidence intervals (CI).



Of 298 study participants, 237 (79.5 %) were female, of which 49 (20.7 %) were pregnant. For the HIV test component, the sensitivity and specificity were 99.2 % (95 % CI: 95.8 %, 100 %) and 97.0 % (95 % CI: 93.2 %, 99.0 %), respectively; and for the syphilis component were 96.5 % (95 % CI: 91.2 %, 99.0 %) and 90.8 % (95 % CI: 85.7 %, 94.6 %), respectively. In pregnant women, the sensitivity and specificity of the HIV test component were 93.3 % (95 % CI: 68.0 %, 99.8 %) and 94.1 % (95 % CI: 80.3 %, 99.3 %), respectively; and for the syphilis component were 100 % (95 % CI:81.5 %, 100 %) and 96.8 % (95 % CI:83.3 %, 99.9 %), respectively.



The Standard Diagnostics BIOLINE HIV/Syphilis Duo dual test performed well in a field setting in Haiti and should be considered for wider use.
Division of Global Public Health, Department of Medicine, University of California San Diego, La Jolla, CA, USA. cbristow@ucsd.edu.|Division of Global Public Health, University of California San Diego, 9500 Gilman Drive 0507, La Jolla, CA, 92093-0507, USA. cbristow@ucsd.edu.
Claire C
CC
Bristow
Les Centres GHESKIO, Port-au-Prince, Haiti.
Linda
L
Severe
Les Centres GHESKIO, Port-au-Prince, Haiti.|Center for Global Health, Division of Infectious Diseases, Department of Medicine, Weill Cornell Medical College, New York, NY, USA.
Jean William
JW
Pape
Department of Epidemiology, University of California Los Angeles, Los Angeles, CA, USA.
Marjan
M
Javanbakht
Department of Epidemiology, University of California Los Angeles, Los Angeles, CA, USA.|Department of Psychiatry and Biobehavioral Sciences, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, USA.
Sung-Jae
SJ
Lee
Department of Psychiatry and Biobehavioral Sciences, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, USA.
Warren Scott
WS
Comulada
Department of Epidemiology, University of California Los Angeles, Los Angeles, CA, USA. JDKlausner@mednet.ucla.edu.|Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, USA. JDKlausner@mednet.ucla.edu.|UCLA David Geffen School of Medicine, Los Angeles, CA, USA. JDKlausner@mednet.ucla.edu.
Jeffrey D
JD
Klausner
D000328:Adult; D000554:Ambulatory Care Facilities; D005260:Female; D015658:HIV Infections / Q000175:diagnosis*; D006205:Haiti; D006801:Humans; D007118:Immunoassay; D008297:Male; D008403:Mass Screening; D010342:Patient Acceptance of Health Care; D000067716:Point-of-Care Testing; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D013587:Syphilis / Q000175:diagnosis*; D013588:Syphilis Serodiagnosis / Q000379:methods
Diagnosis; Point-of-care, HIV, Syphilis, Haiti, Dual test, Rapid test, Dual elimination, Haiti