Highly Selective and Sensitive One- and Two-Photon Ratiometric Fluorescent Probe for Intracellular Hydrogen Polysulfide Sensing.
Hydrogen polysulfide (H2Sn) has attracted increasing attention due to the fact that it is actually the key signaling molecule rather than hydrogen sulfide (H2S). Therefore, developing a sensitive and accurate assay to investigate the biosynthetic pathways of H2Sn is of physiological and pathological significance. In this work, based on the commonly used two-photon fluorophore, 1,8-naphthalimide, a new probe, NRT-HP, has been designed and synthesized that displayed both one- and two-photon ratiometric fluorescence changes toward H2Sn via H2Sn-mediated benzodithiolone formation. NRT-HP exhibits excellent pH stability, high selectivity and low detection limit (0.1 μM) in aqueous media. Furthermore, two-photon fluorescence microscopy experiments have demonstrated that NRT-HP could be used for the H2Sn detection in live cells as well as tissue slices.
Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Special Function Materials and Structure Design, Ministry of Education, College of Chemistry and Chemical Engineering, Lanzhou University , Lanzhou, 730000, China.
Qingxin
Q
Han
Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Special Function Materials and Structure Design, Ministry of Education, College of Chemistry and Chemical Engineering, Lanzhou University , Lanzhou, 730000, China.
Zuolin
Z
Mou
Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Special Function Materials and Structure Design, Ministry of Education, College of Chemistry and Chemical Engineering, Lanzhou University , Lanzhou, 730000, China.
Haihong
H
Wang
Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Special Function Materials and Structure Design, Ministry of Education, College of Chemistry and Chemical Engineering, Lanzhou University , Lanzhou, 730000, China.
Xiaoliang
X
Tang
Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Special Function Materials and Structure Design, Ministry of Education, College of Chemistry and Chemical Engineering, Lanzhou University , Lanzhou, 730000, China.
Zhe
Z
Dong
Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Special Function Materials and Structure Design, Ministry of Education, College of Chemistry and Chemical Engineering, Lanzhou University , Lanzhou, 730000, China.
Li
L
Wang
Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Special Function Materials and Structure Design, Ministry of Education, College of Chemistry and Chemical Engineering, Lanzhou University , Lanzhou, 730000, China.
Xue
X
Dong
Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Special Function Materials and Structure Design, Ministry of Education, College of Chemistry and Chemical Engineering, Lanzhou University , Lanzhou, 730000, China.
Weisheng
W
Liu
D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D057230:Limit of Detection; D008856:Microscopy, Fluorescence / Q000379:methods; D053644:Naphthalimides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D017785:Photons; D012680:Sensitivity and Specificity; D013440:Sulfides / Q000032:analysis*
D005456:Fluorescent Dyes; D053644:Naphthalimides; D013440:Sulfides; C032915:polysulfide