Isolation and characterization of eight novel microsatellite markers in Acanthopagrus schlegelii.
Acanthopagrus schlegelii is a warm temperate demersal fish, which inhabits the sediment substrate or rocky reefs in shallow seas. As this fish is a nutritionally endowed species with good palatability, it is a highly valuable commercial species for aquaculture and has a long historical standing in Western Pacific countries. Because the population of this fish is currently declining in China, studies and measures aimed at addressing this decline are needed. In this study, eight microsatellite markers were screened from 30 wild A. schlegelii fishes through the FIASCO method, whereby sequences containing repeats were obtained from amplified fragment length polymorphisms. The allelic number ranged from 3 to 5, with a mean number of 3.625. The average observed heterozygosity was 0.6290, ranging from 0.3214 to 0.8966, while the expected heterozygosity was 0.5435, ranging from 0.3452 to 0.6721. The value for polymorphism information content ranged from 0.313 to 0.666. These results show this population has moderate genetic variation and low genetic diversity. These novel polymorphic loci will be useful for future genetic studies of A. schlegelii.
Fisheries College, Jimei University, Xiamen, China.|Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, China.
X Q
XQ
Mao
Fisheries College, Jimei University, Xiamen, China.|Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, China.
Z B
ZB
Li
Fisheries College, Jimei University, Xiamen, China.|Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, China.
Y
Y
Yuan
Fisheries College, Jimei University, Xiamen, China.|Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, China.
Y F
YF
Ning
Fisheries College, Jimei University, Xiamen, China.|Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, China.
J B
JB
Shangguan
Fisheries College, Jimei University, Xiamen, China.|Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, China.
Y S
YS
Huang
Fisheries College, Jimei University, Xiamen, China.|Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, China.
M
M
Yang
Fisheries College, Jimei University, Xiamen, China.|Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, China.
B B
BB
Li
D000818:Animals; D004247:DNA; D018895:Microsatellite Repeats / Q000235:genetics; D010473:Perciformes / Q000235:genetics*; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
D004247:DNA