Comprehensive and Quantitative Proteomic Analysis of Metamorphosis-Related Proteins in the Veined Rapa Whelk, Rapana venosa.
Larval metamorphosis of the veined rapa whelk (Rapana venosa) is a pelagic to benthic transition that involves considerable structural and physiological changes. Because metamorphosis plays a pivotal role in R. venosa commercial breeding and natural populations, the endogenous proteins that drive this transition attract considerable interest. This study is the first to perform a comprehensive and quantitative proteomic analysis related to metamorphosis in a marine gastropod. We analyzed the proteomes of competent R. venosa larvae and post-larvae, resulting in the identification of 5312 proteins, including 470 that were downregulated and 668 that were upregulated after metamorphosis. The differentially expressed proteins reflected multiple processes involved in metamorphosis, including cytoskeleton and cell adhesion, ingestion and digestion, stress response and immunity, as well as specific tissue development. Our data improve understanding of the physiological traits controlling R. venosa metamorphosis and provide a solid basis for further study.
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. songhao13@mails.ucas.ac.cn.|University of Chinese Academy of Sciences, Beijing 100049, China. songhao13@mails.ucas.ac.cn.
Hao
H
Song
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. haiyanwang@qdio.ac.cn.
Hai-Yan
HY
Wang
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. tzhang@qdio.ac.cn.
Tao
T
Zhang
D000818:Animals; D019295:Computational Biology / Q000379:methods; D030562:Databases, Protein; D049851:Gastropoda / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D063990:Gene Ontology; D008675:Metamorphosis, Biological / Q000235:genetics; D058977:Molecular Sequence Annotation; D020543:Proteome; D040901:Proteomics / Q000379:methods; D059467:Transcriptome
D020543:Proteome
Rapana venosa; digital gene expression; gastropod; larva; transcriptome