Ethylene-Induced Vinblastine Accumulation Is Related to Activated Expression of Downstream TIA Pathway Genes in Catharanthus roseus.
We selected different concentrations of ethephon, to stress C. roseus. We used qRT-PCR and HPLC followed by PCA to obtain comprehensive profiling of the vinblastine biosynthesis in response to ethephon. Based on our findings, the results showed that the high concentration of ethephon had a positive effect at both transcriptional and metabolite level. Meanwhile, there was a remarkable decrease of hydrogen peroxide content and a promoted peroxidase activity in leaves. The loading plot combination with correlation analysis suggested that CrPrx1 could be regarded as a positive regulator and interacts with ethylene response factor (ERF) to play a key role in vinblastine content and peroxidase (POD) activity. This study provides the foundation for a better understanding of the regulation and accumulation of vinblastine in response to ethephon.
Key Laboratory of Forest Plant Ecology, Northeast Forestry University, Harbin 150040, China.
Xi
X
Wang
Key Laboratory of Forest Plant Ecology, Northeast Forestry University, Harbin 150040, China.
Ya-Jie
YJ
Pan
College of Life Science, Northeast Forestry University, Harbin 150040, China.
Bo-Wen
BW
Chang
College of Life Science, Northeast Forestry University, Harbin 150040, China.
Yan-Bo
YB
Hu
Key Laboratory of Forest Plant Ecology, Northeast Forestry University, Harbin 150040, China.
Xiao-Rui
XR
Guo
Key Laboratory of Forest Plant Ecology, Northeast Forestry University, Harbin 150040, China.
Zhong-Hua
ZH
Tang
D030008:Catharanthus / Q000235:genetics* / Q000378:metabolism; D005030:Ethylenes / Q000032:analysis / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D017343:Genes, Plant / Q000235:genetics*; D009943:Organophosphorus Compounds / Q000494:pharmacology; D010084:Oxidation-Reduction; D014747:Vinblastine / Q000032:analysis / Q000378:metabolism*
D005030:Ethylenes; D009943:Organophosphorus Compounds; D014747:Vinblastine; C036216:ethylene; C005073:ethephon