Generating a host range-expanded recombinant baculovirus.
As baculoviruses usually have a narrow insecticidal spectrum, knowing the mechanisms by which they control the host-range is prerequisite for improvement of their applications as pesticides. In this study, from supernatant of culture cells transfected with DNAs of an Autographa californica multiple nucleopolyhedrovirus (AcMNPV) mutant lacking the antiapoptotic gene p35 (vAc(∆P35)) and a cosmid representing a fragment of Spodoptera exigua nucleopolyhedrovirus (SeMNPV), a viral strain was plaque-purified and named vAcRev. vAcRev had a broader host range than either vAc(∆P35) or SeMNPV parental virus, being able to infect not only the permissive hosts of its parental viruses but also a nonpermissive host (Spodoptera litura). Genome sequencing indicated that vAcRev comprises a mixture of two viruses with different circular dsDNA genomes. One virus contains a genome similar to vAc(∆P35), while in the other viral genome, a 24.4 kbp-fragment containing 10 essential genesis replaced with a 4 kbp-fragment containing three SeMNPV genes including a truncated Se-iap3 gene. RNA interference and ectopic expression assays found that Se-iap3 is responsible for the host range expansion of vAcRev, suggesting that Se-iap3 inhibits the progression of apoptosis initiated by viral infection and promotes viral propagation in hosts both permissive and non-permissive for AcMNPV and SeMNPV.
State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, China.|Liuzhou People's Hospital, Liuzhou 545006, China.
Chunfeng
C
Wu
State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, China.
Zihao
Z
Deng
State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, China.
Zhao
Z
Long
State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, China.
Yi
Y
Cai
State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, China.
Zhongfu
Z
Ying
State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, China.
Hanqi
H
Yin
State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, China.
Meijin
M
Yuan
Division of Biology, Kansas State University, Manhattan, KS66506, USA.
Rollie J
RJ
Clem
State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, China.
Kai
K
Yang
State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, China.
Yi
Y
Pang
D000818:Animals; D003360:Cosmids / Q000235:genetics / Q000378:metabolism; D004279:DNA, Viral / Q000737:chemistry / Q000378:metabolism; D016679:Genome, Viral / Q000235:genetics; D058507:Host Specificity / Q000502:physiology*; D051034:Inhibitor of Apoptosis Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D007915:Lepidoptera / Q000821:virology; D017924:Nucleopolyhedroviruses / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D034622:RNA Interference; D017422:Sequence Analysis, DNA; D061987:Sf9 Cells / Q000166:cytology / Q000821:virology; D018411:Spodoptera / Q000821:virology*; D014764:Viral Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D014779:Virus Replication
D004279:DNA, Viral; D051034:Inhibitor of Apoptosis Proteins; D014764:Viral Proteins; C492234:p35 protein, Baculovirus