Highly efficient detection of paclobutrazol in environmental water and soil samples by time-resolved fluoroimmunoassay.
A fast and ultrasensitive indirect competitive time-resolved fluoroimmunoassay (TRFIA) was developed for the analysis of paclobutrazol in environmental water and soil samples. Paclobutrazol hapten was synthesized and conjugated to bovine serum albumin (BSA) for producing polyclonal antibodies. Under optimal conditions, the 50% inhibitory concentration (IC50 value) and limit of detection (LOD, IC20 value) were 1.09μgL(-1) and 0.067μgL(-1), respectively. The LOD of TRFIA was improved 30-fold compared to the already reported ELISA. There was almost no cross-reactivity of the antibody with the other structural analogues of triazole compounds, indicating that the antibody had high specificity. The average recoveries from spiked samples were in the range from 80.2% to 104.7% with a relative standard deviation of 1.0-9.5%. The TRFIA results for the real samples were in good agreement with that obtained by high-performance liquid chromatography analyses. The results indicate that the established TRFIA has potential application for screening paclobutrazol in environmental samples.
School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address: lzj1984@ujs.edu.cn.
Zhenjiang
Z
Liu
School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China; The Affiliated First People's Hospital of Jiangsu University, Zhenjiang 212002, China.
Xi
X
Wei
School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China.
Kewei
K
Ren
School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China.
Gangbing
G
Zhu
School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China.
Zhen
Z
Zhang
School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China.
Jiagao
J
Wang
School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China.
Daolin
D
Du
D004784:Environmental Monitoring / Q000379:methods*; D015200:Fluoroimmunoassay / Q000379:methods*; D006241:Haptens / Q000737:chemistry; D006540:Herbicides / Q000032:analysis*; D014230:Triazoles / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis*
D006241:Haptens; D006540:Herbicides; D014230:Triazoles; D014874:Water Pollutants, Chemical; C053370:paclobutrazol
Environmental samples; Enzyme-linked immunosorbent assay; Hapten; Paclobutrazol; Polyclonal antibody; Time-resolved fluoroimmunoassay