Enzymatic Synthesis of 6-O-α-D-Galactopyranosyl-1-deoxynojirimycin Using α-Galactosidase from Green Coffee Beans.
A transgalactosylation reaction from p-nitrophenyl-α-D-galactopyranoside to 1-deoxynojirimycin was done using α-galactosidase [EC 3.2.1.22] from green coffee beans. The enzyme formed 6-O-α-D-galactopyranosyl-1-deoxynojirimycin as the major product.
a IlDong Pharmaceutical Co., Research Laboratories.
N S
NS
Paek
a IlDong Pharmaceutical Co., Research Laboratories.
D J
DJ
Kang
a IlDong Pharmaceutical Co., Research Laboratories.
H S
HS
Lee
b Korea Research Institute of Bioscience and Biotechnology.
J J
JJ
Lee
c Graduate School of Biotechnology, Korea University.
Y J
YJ
Choi
a IlDong Pharmaceutical Co., Research Laboratories.
T H
TH
Kim
a IlDong Pharmaceutical Co., Research Laboratories.
K W
KW
Kim
1-deoxynojirimycin; 6-O-α-D-galactopyranosyl-1-deoxynojirimycin; α-galactosidase