Staphylococcus aureus detection in blood samples by silica nanoparticle-oligonucleotides conjugates.
A fast, specific and sensitive homogeneous assay for Staphylococcus aureus detection was developed by measuring the activity of secreted nuclease from the bacteria via a modified DNA oligonucleotide. As biosensor format, an effective system, Nanokeepers as previously reported, were used for triggered release of confined fluorophores, and hence specific detection of S. aureus on nuclease activity was obtained. The interference from blood components for fluorescent quantification was eliminated by a pre-purification by aptamer-functionalized silica magnetic nanoparticles. The reported assay system was exclusively formed by nucleic acid oligos and magnetic or mesoporous silica nanoparticles, that can be used on blood samples in a stepwise manner. The assay was successfully used as a sensing platform for the specific detection of S. aureus cells as low as 682 CFU in whole blood.
Istanbul Kemerburgaz University, School of Medicine, Istanbul 34217, Turkey. Electronic address: cengizozalp@gmail.com.
Baris A
BA
Borsa
Yeditepe University, School of Medicine, Department of Biophysics, Istanbul Turkey. Electronic address: Bilgeguv@gmail.com.
Bilge G
BG
Tuna
SOMAprobes S.L., Science and Technology Park of Gipuzkoa, San Sebastian 20009, Spain. Electronic address: frank.hernandez@somaprobes.com.
Frank J
FJ
Hernandez
SOMAprobes S.L., Science and Technology Park of Gipuzkoa, San Sebastian 20009, Spain. Electronic address: luiza.hernandez@somaprobes.com.
Luiza I
LI
Hernandez
Biochemical Processing and Biomaterial Research Laboratory, Gazi University, 06500 Ankara, Turkey; Department of Chemistry, Faculty of Sciences, Gazi University, 06500 Ankara, Turkey. Electronic address: g_bayramoglu@gmail.com.
Gulay
G
Bayramoglu
Biochemical Processing and Biomaterial Research Laboratory, Gazi University, 06500 Ankara, Turkey. Electronic address: yakuparica@tnn.net.
M Yakup
MY
Arica
Istanbul Kemerburgaz University, School of Medicine, Istanbul 34217, Turkey.
V Cengiz
VC
Ozalp
D016470:Bacteremia / Q000097:blood* / Q000175:diagnosis*; D015373:Bacterial Typing Techniques / Q000295:instrumentation*; D001774:Blood Chemical Analysis / Q000295:instrumentation; D015342:DNA Probes / Q000235:genetics*; D004269:DNA, Bacterial / Q000097:blood* / Q000235:genetics / Q000302:isolation & purification; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry; D058726:Nanoconjugates / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry; D013211:Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification*
D015342:DNA Probes; D004269:DNA, Bacterial; D058185:Magnetite Nanoparticles; D058726:Nanoconjugates; D012822:Silicon Dioxide
Aptamers; Magnetic separation; Micrococcal nuclease; Nanoparticles; Staphylococcus aureus