Impact of air pollution on oxidative DNA damage and lipid peroxidation in mothers and their newborns.
Ambient air particulate matter (PM) represents a class of heterogeneous substances that form one component of air pollution. Oxidative stress has been implicated as an important action mechanism for PM on the human organism. Oxidative damage induced by reactive oxygen species (ROS) may affect any cellular macromolecule. The aim of our study was to investigate the impact of air pollution on oxidative DNA damage [8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG)] and lipid peroxidation [15-F2t-isoprostane (15-F2t-IsoP)] in the urine and blood from mothers and newborns from two localities with different levels of air pollution: Ceske Budejovice (CB), a locality with a clean air, and Karvina, a locality with high air pollution. The samples from normal deliveries (38-41 week+) of nonsmoking mothers and their newborns were collected in the summer and winter seasons. Higher PM2.5 concentrations were found in Karvina than in CB in the summer 2013 (mean±SD: 20.41±6.28 vs. 9.45±3.62μg/m(3), P<0.001), and in the winter 2014 (mean±SD: 53.67±19.76 vs. 27.96±12.34μg/m(3), P<0.001). We observed significant differences in 15-F2t-IsoP levels between the summer and winter seasons in Karvina for newborns (mean±SD: 64.24±26.75 vs. 104.26±38.18pg/ml plasma, respectively) (P<0.001). Levels of 8-oxodG differed only in the winter season between localities, they were significantly higher (P<0.001) in newborns from Karvina in comparison with CB (mean±SD: 5.70±2.94 vs. 4.23±1.51 nmol/mmol creatinine, respectively). The results of multivariate regression analysis in newborns from Karvina showed PM2.5 concentrations to be a significant predictor for 8-oxodG excretion, PM2.5 and B[a]P (benzo[a]pyrene) concentrations to be a significant predictor for 15-F2t-IsoP levels. The results of multivariate regression analysis in mothers showed PM2.5 concentrations to be a significant predictor of 8-oxodG levels.
Department of Genetic Ecotoxicology, Institute of Experimental Medicine, AS CR, Prague, Czech Republic.
Antonin
A
Ambroz
Department of Genetic Ecotoxicology, Institute of Experimental Medicine, AS CR, Prague, Czech Republic.
Veronika
V
Vlkova
Department of Genetic Ecotoxicology, Institute of Experimental Medicine, AS CR, Prague, Czech Republic.
Pavel
P
Rossner
Department of Genetic Ecotoxicology, Institute of Experimental Medicine, AS CR, Prague, Czech Republic.
Andrea
A
Rossnerova
Department of Genetic Ecotoxicology, Institute of Experimental Medicine, AS CR, Prague, Czech Republic.
Vlasta
V
Svecova
Department of Genetic Ecotoxicology, Institute of Experimental Medicine, AS CR, Prague, Czech Republic.
Alena
A
Milcova
Department of Food Analysis and Nutrition, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Prague, Czech Republic.
Jana
J
Pulkrabova
Department of Food Analysis and Nutrition, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Prague, Czech Republic.
Jana
J
Hajslova
Faculty of Health and Social Studies, University of South Bohemia, Ceske Budejovice, Czech Republic.
Milos
M
Veleminsky
Department of Genetic Ecotoxicology, Institute of Experimental Medicine, AS CR, Prague, Czech Republic.
Ivo
I
Solansky
Department of Genetic Ecotoxicology, Institute of Experimental Medicine, AS CR, Prague, Czech Republic. Electronic address: sram@biomed.cas.cz.
Radim J
RJ
Sram
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D000393:Air Pollutants / Q000032:analysis; D000397:Air Pollution / Q000032:analysis*; D003367:Cotinine / Q000652:urine; D018153:Czech Republic; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000652:urine; D015237:Dinoprost / Q000031:analogs & derivatives; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D028421:Isoprostanes / Q000097:blood*; D015227:Lipid Peroxidation; D008297:Male; D018811:Maternal Exposure; D009035:Mothers; D052638:Particulate Matter / Q000032:analysis; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis; D011247:Pregnancy / Q000097:blood / Q000652:urine; D055815:Young Adult
D000393:Air Pollutants; D028421:Isoprostanes; D052638:Particulate Matter; D011084:Polycyclic Aromatic Hydrocarbons; C075750:8-epi-prostaglandin F2alpha; D000080242:8-Hydroxy-2'-Deoxyguanosine; D015237:Dinoprost; D003849:Deoxyguanosine; D003367:Cotinine
Air pollution; Benzo[a]pyrene; Lipid peroxidation; Newborns; Oxidative DNA damage; Particulate matter