Platycodin D induced apoptosis and autophagy in PC-12 cells through mitochondrial dysfunction pathway.
In this article, the in vitro cytotoxicity of platycodin D was evaluated in human PC-12, SGC-7901, BEL-7402, HeLa and A549 cancer cell lines. PC-12 cells were sensitive to platycodin D treatment, with an IC50 value of 13.5±1.2μM. Morphological and comet assays showed that platycodin D effectively induced apoptosis in PC-12 cells. Platycodin D increased the levels of reactive oxygen species (ROS) and induced a decrease in mitochondrial membrane potential. Platycodin D induced cell cycle arrest at the G0/G1 phase in the PC-12 cell line. Platycodin D can induce autophagy. In addition, platycodin D can down-regulate the expression of Bcl-2 and Bcl-x, and up-regulate the levels of Bid protein in the PC-12 cells. The results demonstrated that platycodin D induced PC-12 cell apoptosis through a ROS-mediated mitochondrial dysfunction pathway.
School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China.
Chuan-Chuan
CC
Zeng
School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China.
Cheng
C
Zhang
Instrumentation Analysis and Research Center, Sun Yat-Sen University, Guangzhou 510275, PR China.
Jun-Hua
JH
Yao
School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China.
Shang-Hai
SH
Lai
School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China.
Bing-Jie
BJ
Han
School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China.
Wei
W
Li
School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China.
Bing
B
Tang
School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China.
Dan
D
Wan
School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China. Electronic address: lyjche@163.com.
Yun-Jun
YJ
Liu
D000072283:A549 Cells; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001343:Autophagy / Q000187:drug effects*; D029422:Campanulaceae / Q000737:chemistry; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D002460:Cell Line; D006367:HeLa Cells; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D009369:Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D016716:PC12 Cells; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D012503:Saponins / Q000737:chemistry / Q000494:pharmacology*; D014315:Triterpenes / Q000737:chemistry / Q000494:pharmacology*
D000972:Antineoplastic Agents, Phytogenic; D017382:Reactive Oxygen Species; D012503:Saponins; D014315:Triterpenes; C108953:platycodin D
Apoptosis; Autophagy; Cytotoxicity in vitro; Platycodin D; ROS