The novel chicken interleukin 26 protein is overexpressed in T cells and induces proinflammatory cytokines.
In the present study, we describe the cloning and functional characterization of chicken interleukin 26 (ChIL-26). ChIL-26, a member of the IL-10 cytokine family, induces the production of proinflammatory cytokines by T cells. The ChIL-26 cDNA encodes an 82-amino-acid protein whose amino acid sequence has 22.63, 46.31 and 43.15% homology with human IL-26, pig IL-26 and canary IL-26, respectively. ChIL-26 signals through a heterodimeric receptor complex composed of the IL-20R1 and IL-10R2 chains, which are expressed primarily in the CU91 T cell line as well as CD4(+) and CD8(+) T cells. Recombinant ChIL-26 protein induced Th1 cytokines (IL-16 and IFN-γ), Th2 cytokines (IL-4, IL-6 and IL-10), Th17 cytokines (IL-17A, IL-17D, and IL-17F), and chemokine transcripts (mainly CCL3, CCL4, CCL5, CCL20 and CXCL13) in the CU91 T cell line and in CD4(+) and CD8(+) T cells, however IL-18 was not expressed in the CU91 T cell line. Taken together, the data demonstrates that T cells express the functional ChIL-26 receptor complex and that ChIL-26 modulates T cell proliferation and proinflammatory gene expression. To the best of our knowledge, this is the first report of cloned ChIL-26. We evaluated its functional roles, particularly in the pathogenic costimulation of T cells, which may be significantly associated with the induction of cytokines.
Department of Animal Science and Technology, Chung-Ang University, Anseong, 17546, Republic of Korea.
Anh Duc
AD
Truong
Department of Animal Science and Technology, Chung-Ang University, Anseong, 17546, Republic of Korea.
Boyeong
B
Park
Department of Animal Science and Technology, Chung-Ang University, Anseong, 17546, Republic of Korea.
Jihye
J
Ban
Department of Animal Science and Technology, Chung-Ang University, Anseong, 17546, Republic of Korea. yhong@cau.ac.kr.
Yeong Ho
YH
Hong
D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D002171:Canaries / Q000235:genetics / Q000276:immunology; D002645:Chickens / Q000276:immunology / Q000378:metabolism*; D003001:Cloning, Molecular; D016207:Cytokines / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000235:genetics / Q000502:physiology; D006801:Humans; D007378:Interleukins / Q000235:genetics / Q000502:physiology*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D017385:Sequence Homology; D013552:Swine / Q000235:genetics / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*
D016207:Cytokines; C406767:IL26 protein, human; D007378:Interleukins