Apoptosis in response to heat stress is positively associated with heat-shock protein 90 expression in chicken myocardial cellsin vitro.
To determine heat-shock protein (Hsp)90 expression is connected with cellular apoptotic response to heat stress and its mechanism, chicken (Gallus gallus) primary myocardial cells were treated with the Hsp90 promoter, aspirin, and its inhibitor, geldanamycin (GA), before heat stress. Cellular viability, heat-stressed apoptosis and reactive oxygen species level under different treatments were measured, and the expression of key proteins of the signaling pathway related to Hsp90 and their colocalization with Hsp90 were detected. The results showed that aspirin treatment increased the expression of protein kinase B (Akt), the signal transducer and activator of transcription (STAT)-3 and p-IKKα/β and the colocalization of Akt and STAT-3 with Hsp90 during heat stress, which was accompanied by improved viability and low apoptosis. GA significantly inhibited Akt expression and p-IKKα/β level, but not STAT-3 quantity, while the colocalization of Akt and STAT-3 with Hsp90 was weakened, followed by lower cell viability and higher apoptosis. Aspirin after GA treatment partially improved the stress response and apoptosis rate of tested cells caused by the recovery of Akt expression and colocalization, rather than the level of STAT-3 (including its co-localization with Hsp90) and p-IKKα/β. Therefore, Hsp90 expression has a positive effect on cellular capacity to resist heat-stressed injury and apoptosis. Moreover, inhibition of Hsp90 before stress partially attenuated its positive effects.
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
Xiao-Hui
XH
Zhang
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
Hong
H
Wu
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
Shu
S
Tang
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
Qiao-Ning
QN
Li
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
Jiao
J
Xu
College of Animal Science and Technology, Jinling Institute of Technology, Nanjing 210038, China.
Miao
M
Zhang
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
Ya-Nan
YN
Su
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
Bin
B
Yin
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
Qi-Ling
QL
Zhao
Institute for Animal Hygiene, Animal Welfare and Farm Animal Behaviour, University of Veterinary Medicine Hannover, Foundation, Hannover 30173, Germany.
Nicole
N
Kemper
Institute for Animal Hygiene, Animal Welfare and Farm Animal Behaviour, University of Veterinary Medicine Hannover, Foundation, Hannover 30173, Germany.
Joerg
J
Hartung
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
En-Dong
ED
Bao
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001241:Aspirin / Q000494:pharmacology; D016227:Benzoquinones / Q000494:pharmacology; D015153:Blotting, Western / Q000662:veterinary; D002642:Chick Embryo / Q000166:cytology; D005434:Flow Cytometry / Q000662:veterinary; D018841:HSP90 Heat-Shock Proteins / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism* / Q000502:physiology; D018869:Heat-Shock Response / Q000502:physiology*; D066298:In Vitro Techniques; D047029:Lactams, Macrocyclic / Q000494:pharmacology; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism
D016227:Benzoquinones; D018841:HSP90 Heat-Shock Proteins; D047029:Lactams, Macrocyclic; D017382:Reactive Oxygen Species; D001241:Aspirin; C001277:geldanamycin
Hsp90 heat-shock proteins; anti-apoptosis; chicken primary myocardial cells; heat stress disorders