Measuring the effects of fractionated radiation therapy in a 3D prostate cancer model system using SERS nanosensors.
Multicellular tumour spheroids (MTS) are three-dimensional cell cultures that possess their own microenvironments and provide a more meaningful model of tumour biology than monolayer cultures. As a result, MTS are becoming increasingly used as tumor models when measuring the efficiency of therapies. Monitoring the viability of live MTS is complicated by their 3D nature and conventional approaches such as fluorescence often require fixation and sectioning. In this paper we detail the use of Surface Enhanced Raman Spectroscopy (SERS) to measure the viability of MTS grown from prostate cancer (PC3) cells. Our results show that we can monitor loss of viability by measuring pH and redox potential in MTS and furthermore we demonstrate that SERS can be used to measure the effects of fractionation of a dose of radiotherapy in a way that has potential to inform treatment planning.
School of Chemistry, University of Edinburgh, Edinburgh, EH9 3FJ, UK. colin.campbell@ed.ac.uk.
Victoria L
VL
Camus
Grant D
GD
Stewart
Edinburgh Radiation Research Collaborative, Oncology Physics, Western General Hospital, Edinburgh EH4 2U, UK.
William H
WH
Nailon
Edinburgh Radiation Research Collaborative, Edinburgh Cancer Centre, Western General Hospital, Edinburgh EH4 2U, UK.
Duncan B
DB
McLaren
School of Chemistry, University of Edinburgh, Edinburgh, EH9 3FJ, UK. colin.campbell@ed.ac.uk.
Colin J
CJ
Campbell
D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D019583:Dose Fractionation, Radiation; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000532:radiotherapy*; D013059:Spectrum Analysis, Raman; D018874:Spheroids, Cellular / Q000528:radiation effects*