Chicken rRNA Gene Cluster Structure.
Ribosomal RNA (rRNA) genes, whose activity results in nucleolus formation, constitute an extremely important part of genome. Despite the extensive exploration into avian genomes, no complete description of avian rRNA gene primary structure has been offered so far. We publish a complete chicken rRNA gene cluster sequence here, including 5'ETS (1836 bp), 18S rRNA gene (1823 bp), ITS1 (2530 bp), 5.8S rRNA gene (157 bp), ITS2 (733 bp), 28S rRNA gene (4441 bp) and 3'ETS (343 bp). The rRNA gene cluster sequence of 11863 bp was assembled from raw reads and deposited to GenBank under KT445934 accession number. The assembly was validated through in situ fluorescent hybridization analysis on chicken metaphase chromosomes using computed and synthesized specific probes, as well as through the reference assembly against de novo assembled rRNA gene cluster sequence using sequenced fragments of BAC-clone containing chicken NOR (nucleolus organizer region). The results have confirmed the chicken rRNA gene cluster validity.
Saint Petersburg State University, Saint Petersburg, 199034, Russia.
Alexander G
AG
Dyomin
Saint Petersburg State University, Saint Petersburg, 199034, Russia.
Elena I
EI
Koshel
Almazov Federal Medical Research Centre, Saint Petersburg, 197341, Russia.|ITMO University, Saint Petersburg, 197101, Russia.
Artem M
AM
Kiselev
Saint Petersburg State University, Saint Petersburg, 199034, Russia.
Alsu F
AF
Saifitdinova
Saint Petersburg State University, Saint Petersburg, 199034, Russia.
Svetlana A
SA
Galkina
Osaka University, Suita, Osaka, 565-0871, Japan.
Tatsuo
T
Fukagawa
Almazov Federal Medical Research Centre, Saint Petersburg, 197341, Russia.|ITMO University, Saint Petersburg, 197101, Russia.
Anna A
AA
Kostareva
Saint Petersburg State University, Saint Petersburg, 199034, Russia.
Elena R
http://orcid.org/0000-0003-4967-9053
ER
Gaginskaya
D000818:Animals; D002645:Chickens / Q000235:genetics*; D005810:Multigene Family; D012337:RNA, Ribosomal, 18S / Q000235:genetics*; D012339:RNA, Ribosomal, 28S / Q000235:genetics*; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics*
D012337:RNA, Ribosomal, 18S; D012339:RNA, Ribosomal, 28S; D012340:RNA, Ribosomal, 5.8S