Bioprospecting the Curculigoside-Cinnamic Acid-Rich Fraction from Molineria latifolia Rhizome as a Potential Antioxidant Therapeutic Agent.
Increasing evidence from both experimental and clinical studies depicts the involvement of oxidative stress in the pathogenesis of various diseases. Specifically, disruption of homeostatic redox balance in accumulated body fat mass leads to obesity-associated metabolic syndrome. Strategies for the restoration of redox balance, potentially by exploring potent plant bioactives, have thus become the focus of therapeutic intervention. The present study aimed to bioprospect the potential use of the curculigoside-cinnamic acid-rich fraction from Molineria latifolia rhizome as an antioxidant therapeutic agent. The ethyl acetate fraction (EAF) isolated from M. latifolia rhizome methanolic extract (RME) contained the highest amount of phenolic compounds, particularly curculigoside and cinnamic acid. EAF demonstrated glycation inhibitory activities in both glucose- and fructose-mediated glycation models. In addition, in vitro chemical-based and cellular-based antioxidant assays showed that EAF exhibited high antioxidant activities and a protective effect against oxidative damage in 3T3-L1 preadipocytes. Although the efficacies of individual phenolics differed depending on the structure and concentration, a correlational study revealed strong correlations between total phenolic contents and antioxidant capacities. The results concluded that enriched phenolic contents in EAF (curculigoside-cinnamic acid-rich fraction) contributed to the overall better reactivity. Our data suggest that this bioactive-rich fraction warrants therapeutic potential against oxidative stress-related disorders.
Nutri-Cosmeceuticals, Nutrigenomics &amp; Nanodelivery Programme, Laboratory of Molecular Biomedicine, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Malaysia. ooiderjiun@gmail.com.
Der Jiun
DJ
Ooi
Nutri-Cosmeceuticals, Nutrigenomics &amp; Nanodelivery Programme, Laboratory of Molecular Biomedicine, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Malaysia. chankim@upm.edu.my.
Kim Wei
KW
Chan
Nutri-Cosmeceuticals, Nutrigenomics &amp; Nanodelivery Programme, Laboratory of Molecular Biomedicine, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Malaysia. sarega5166@gmail.com.
Nadarajan
N
Sarega
Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Malaysia. noorjahan@upm.edu.my.
Noorjahan Banu
NB
Alitheen
Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Malaysia. hairuszah@upm.edu.my.
Hairuszah
H
Ithnin
Nutri-Cosmeceuticals, Nutrigenomics &amp; Nanodelivery Programme, Laboratory of Molecular Biomedicine, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Malaysia. maznahis@upm.edu.my.|Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Malaysia. maznahis@upm.edu.my.
Maznah
M
Ismail
D041721:3T3-L1 Cells; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000737:chemistry*; D001565:Benzoates / Q000008:administration & dosage / Q000737:chemistry; D002934:Cinnamates / Q000008:administration & dosage / Q000737:chemistry; D005419:Flavonoids / Q000737:chemistry; D005960:Glucosides / Q000008:administration & dosage / Q000737:chemistry; D000070476:Hypoxidaceae / Q000737:chemistry*; D051379:Mice; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000737:chemistry; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry*; D027343:Rhizome / Q000737:chemistry
D000975:Antioxidants; D001565:Benzoates; D002934:Cinnamates; D005419:Flavonoids; D005960:Glucosides; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; C029010:cinnamic acid; C477170:curculigoside
3T3-L1 preadipocytes; Molineria latifolia rhizome; antioxidant activity; curculigoside-cinnamic acid-rich fraction; ethyl acetate fraction; oxidative stress