Isoform-specific modulation of the chemical sensitivity of conserved TRPA1 channel in the major honeybee ectoparasitic mite, Tropilaelaps mercedesae.
We identified and characterized the TRPA1 channel of Tropilaelaps mercedesae (TmTRPA1), one of two major species of honeybee ectoparasitic mite. Three TmTRPA1 isoforms with unique N-terminal sequences were activated by heat, and the isoform highly expressed in the mite's front legs, TmTRPA1b, was also activated by 27 plant-derived compounds including electrophiles. This suggests that the heat- and electrophile-dependent gating mechanisms as nocisensitive TRPA1 channel are well conserved between arthropod species. Intriguingly, one TmTRPA1 isoform, TmTRPA1a, was activated by only six compounds compared with two other isoforms, demonstrating that the N-terminal sequences are critical determinants for the chemical sensitivity. This is the first example of isoform-specific modulation of chemical sensitivity of TRPA1 channel in one species. α-terpineol showed repellent activity towards T. mercedesae in a laboratory assay and repressed T. mercedesae entry for reproduction into the brood cells with fifth instar larvae in hives. Thus, α-terpineol could be used as the potential compound to control two major honeybee ectoparasitic mites, T. mercedesae and Varroa destructor, in the apiculture industry.
Department of Biological Sciences, Xi'an Jiaotong-Liverpool University, 111 Ren'ai Road, Suzhou Dushu Lake Higher Education Town, Jiangsu Province 215123, People's Republic of China.
Xiaofeng
X
Dong
Division of Cell Signaling, Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Okazaki 444-8787, Japan.
Makiko
M
Kashio
Department of Biological Sciences, Xi'an Jiaotong-Liverpool University, 111 Ren'ai Road, Suzhou Dushu Lake Higher Education Town, Jiangsu Province 215123, People's Republic of China.
Guangda
G
Peng
Department of Biological Sciences, Xi'an Jiaotong-Liverpool University, 111 Ren'ai Road, Suzhou Dushu Lake Higher Education Town, Jiangsu Province 215123, People's Republic of China.
Xinyue
X
Wang
Division of Cell Signaling, Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Okazaki 444-8787, Japan Department of Physiological Sciences, SOKENDAI (The Graduate University for Advanced Studies), Okazaki 444-8585, Japan.
Makoto
M
Tominaga
Department of Biological Sciences, Xi'an Jiaotong-Liverpool University, 111 Ren'ai Road, Suzhou Dushu Lake Higher Education Town, Jiangsu Province 215123, People's Republic of China tatsuhiko.kadowaki@xjtlu.edu.cn.
Tatsuhiko
0000-0002-4622-1218
T
Kadowaki
D017398:Alternative Splicing; D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics / Q000378:metabolism; D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D008925:Mites / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D039821:Monoterpenes / Q000494:pharmacology; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000074025:TRPA1 Cation Channel / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D014018:Tissue Distribution
D060829:Arthropod Proteins; D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes; D039821:Monoterpenes; D020033:Protein Isoforms; D000074025:TRPA1 Cation Channel; C016775:alpha-terpineol
TRPA1 channel; honeybee decline; honeybee ectoparasitic mite; plant oil