Regulated Oxygenation of Rewarming Machine Perfusion for Porcine Donation After Cardiac Death Liver Transplantation.
BACKGROUND
Machine perfusion (MP) is particularly expected to preserve and resuscitate an organ obtained from extended criteria donors or donation after cardiac death to expand the donated organ pool for organ transplantation. This method requires to be investigated an optimal preservation condition. The aim of this study is investigation of the optimal oxygenation conditions under rewarming MP (RMP).


METHODS
Porcine livers were perfused with an RMP system developed by our research group. All livers were procured under warm ischemia time of 60 minutes, and preserved in static cold storage for 2 hours, and perfused for 2 hours using the RMP. For group 1, the livers were supplied with oxygen constantly through perfusate. For group 2, the livers were supplied with oxygen increasingly with controlling flow rates and oxygen concentration. Effluent enzymes were obtained during perfusion preservation.


RESULTS
The average levels of alanine aminotransferase were lower in group 2 than in group 1 during RMP, and also decreasing the hepatic artery pressures after 60 minutes.


CONCLUSIONS
Regulated oxygenation of RMP has possibility to improve the graft preservation for liver transplantation.
Mechanical Engineering, Tokyo Metropolitan University, Tokyo, Japan.
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N
Morito
Mechanical Engineering, Tokyo Metropolitan University, Tokyo, Japan. Electronic address: obara@tmu.ac.jp.
H
H
Obara
Mechanical Engineering, Tokyo Metropolitan University, Tokyo, Japan; Department of Surgery, Asahikawa Medical University, Asahikawa, Japan.
N
N
Matsuno
Advanced Medical Science, National Center for Child Health and Development, Tokyo, Japan.
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S
Enosawa
Department of Surgery, Asahikawa Medical University, Asahikawa, Japan.
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K
Watanabe
Department of Surgery, Asahikawa Medical University, Asahikawa, Japan.
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M
Furukori
Department of Surgery, Asahikawa Medical University, Asahikawa, Japan.
H
H
Furukawa
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D015925:Cryopreservation / Q000379:methods*; D003643:Death; D005260:Female; D008099:Liver / Q000098:blood supply* / Q000378:metabolism; D016031:Liver Transplantation; D009926:Organ Preservation / Q000295:instrumentation / Q000379:methods*; D010477:Perfusion / Q000295:instrumentation / Q000379:methods*; D017694:Rewarming / Q000295:instrumentation / Q000379:methods*; D034421:Sus scrofa; D014019:Tissue Donors; D052096:Warm Ischemia
D015415:Biomarkers