The Influence of Bone Marrow-Secreted IL-10 in a Mouse Model of Cerulein-Induced Pancreatic Fibrosis.
This study aimed to understand the role of IL-10 secreted from bone marrow (BM) in a mouse model of pancreatic fibrosis. The severity of cerulein-induced inflammation, fibrosis, and the frequency of BM-derived myofibroblasts were evaluated in the pancreas of mice receiving either a wild-type (WT) BM or an IL-10 knockout (KO) BM transplantation. The area of collagen deposition increased significantly in the 3 weeks after cerulein cessation in mice with an IL-10 KO BM transplant (13.7 ± 0.6% and 18.4 ± 1.1%, p < 0.05), but no further increase was seen in WT BM recipients over this time. The percentage of BM-derived myofibroblasts also increased in the pancreas of the IL-10 KO BM recipients after cessation of cerulein (6.7 ± 1.1% and 11.9 ± 1.3%, p < 0.05), while this figure fell in WT BM recipients after cerulein withdrawal. Furthermore, macrophages were more numerous in the IL-10 KO BM recipients than the WT BM recipients after cerulein cessation (23.2 ± 2.3 versus 15.3 ± 1.7 per HPF, p < 0.05). In conclusion, the degree of fibrosis, inflammatory cell infiltration, and the number of BM-derived myofibroblasts were significantly different between IL-10 KO BM and WT BM transplanted mice, highlighting a likely role of IL-10 in pancreatitis.
Department of Gastroenterology and Hepatology, Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Taoyuan 333, Taiwan.
Wey-Ran
WR
Lin
Department of Neurology, Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Taoyuan 333, Taiwan.
Siew-Na
SN
Lim
Department of Nephrology, Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Taoyuan 333, Taiwan.
Tzung-Hai
TH
Yen
Centre for Tumour Biology, Barts and The London School of Medicine and Dentistry, London EC1M 6BQ, UK.
Malcolm R
MR
Alison
D000818:Animals; D016026:Bone Marrow Transplantation; D002569:Cerulenin / Q000633:toxicity; D003094:Collagen / Q000378:metabolism; D005355:Fibrosis / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology; D016753:Interleukin-10 / Q000235:genetics*; D051379:Mice; D018345:Mice, Knockout; D058628:Myofibroblasts / Q000187:drug effects / Q000473:pathology; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology
C556062:IL10 protein, mouse; D016753:Interleukin-10; D002569:Cerulenin; D003094:Collagen