The Degradation Interface of Magnesium Based Alloys in Direct Contact with Human Primary Osteoblast Cells.
Magnesium alloys have been identified as a new generation material of orthopaedic implants. In vitro setups mimicking physiological conditions are promising for material / degradation analysis prior to in vivo studies however the direct influence of cell on the degradation mechanism has never been investigated. For the first time, the direct, active, influence of human primary osteoblasts on magnesium-based materials (pure magnesium, Mg-2Ag and Mg-10Gd alloys) is studied for up to 14 days. Several parameters such as composition of the degradation interface (directly beneath the cells) are analysed with a scanning electron microscope equipped with energy dispersive X-ray and focused ion beam. Furthermore, influence of the materials on cell metabolism is examined via different parameters like active mineralisation process. The results are highlighting the influences of the selected alloying element on the initial cells metabolic activity.
Division of Metallic Biomaterials, Institute of Material Research, Helmholtz-Zentrum Geesthacht, Geesthacht, Germany.
Nezha
http://orcid.org/0000-0003-0368-0606
N
Ahmad Agha
Division of Metallic Biomaterials, Institute of Material Research, Helmholtz-Zentrum Geesthacht, Geesthacht, Germany.
Regine
R
Willumeit-Römer
Division of Materials Physics, Institute of Material Research, Helmholtz-Zentrum Geesthacht, Geesthacht, Germany.
Daniel
D
Laipple
Division of Metallic Biomaterials, Institute of Material Research, Helmholtz-Zentrum Geesthacht, Geesthacht, Germany.
Bérengère
B
Luthringer
Division of Metallic Biomaterials, Institute of Material Research, Helmholtz-Zentrum Geesthacht, Geesthacht, Germany.
Frank
F
Feyerabend
D000497:Alloys / Q000737:chemistry* / Q000494:pharmacology; D002113:Calcification, Physiologic / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D008274:Magnesium / Q000737:chemistry* / Q000494:pharmacology; D008422:Materials Testing; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D013052:Spectrometry, X-Ray Emission
D000497:Alloys; D008274:Magnesium