Salvianolic Acid B Restored Impaired Barrier Function via Downregulation of MLCK by microRNA-1 in Rat Colitis Model.
Salvianolic acid B (Sal B) is isolated from the traditional Chinese medical herb Salvia miltiorrhiza and is reported to have a wide range of therapeutic benefits. The aim of this study was to investigate the effects of Sal B on epithelial barrier dysfunction in rat colitis and to uncover related mechanisms. Rat colitis model was established by intracolonic administration of 2, 4, 6-trinitrobenzene sulfonic acid (TNBS). The intestinal barrier function was evaluated by measuring the serum recovery of fluorescein isothiocyanate-4 kD dextran in vivo and transepithelial electrical resistance in vitro respectively. The protein expression related to intestinal barrier function was studied using western blotting. The effects of Sal B on inflammatory responses, oxidative damage and colitis recurrence were also studied in this study. The intestinal barrier dysfunction in colitis was reversed by Sal B, accompanied with the decrease of tight junction proteins, and the effect could be blocked by microRNA-1(miR-1) inhibition. The inflammatory responses, oxidative damage and colitis recurrence were also decreased by Sal B. The colitis symptoms and recurrences were ameliorated by Sal B, and restoration of impaired barrier function via downregulation of MLCK by miR-1 maybe involved in this effect. This study provides some novel insights into both of the pathological mechanisms and treatment alternatives of inflammatory bowel disease.
Central Laboratory, The First Affiliated Hospital, Dalian Medical University Dalian, China.
Yongjian
Y
Xiong
Laboratory Animal Center, Dalian Medical University Dalian, China.
Jingyu
J
Wang
Institute for Brain Disorder, College of Basic Medical Sciences, Dalian Medical University Dalian, China.
Hongwei
H
Chu
Laboratory Animal Center, Dalian Medical University Dalian, China.
Dapeng
D
Chen
Central Laboratory, The First Affiliated Hospital, Dalian Medical University Dalian, China.
Huishu
H
Guo
barrier function; inflammatory bowel disease; microRNA-1; myosin light chain kinase; salvianolic acid B