Helicobacter pylori is undetectable in intraductal papillary mucinous neoplasm.
BACKGROUND
About half of the world population is infected with Helicobacter pylori (H. pylori), a bacterium associated with gastric cancer and considered to be a risk factor for pancreatic ductal adenocarcinoma. Whether the bacterium is associated with intraductal papillary mucinous neoplasm, believed to be a precursor of pancreatic ductal adenocarcinoma, is unknown. The aim of this study was to investigate the presence of H. pylori DNA in tissue sections of intraductal papillary mucinous neoplasm.


METHODS
The presence of H. pylori DNA was tested in a retrospective controlled study of formalin-fixed, paraffin-embedded pancreatic tissues from 24 patients who underwent surgery for intraductal papillary mucinous neoplasm. Histologically normal tissues surrounding neoplasms were used as control. H. pylori DNA was evaluated after deparaffinization, DNA extraction, and purification, and results were evaluated statistically.


RESULTS
Samples were collected from 13 males and 11 females with mean age 59 years (range 44-77), and consisted of 19 cases of main-duct and three cases of branched-duct intraductal papillary mucinous neoplasm. Two patients were diagnosed with pancreatic cancer and main-duct intraductal papillary mucinous neoplasm. H. pylori DNA was not detected either in intraductal papillary mucinous neoplasm tissue, or in surrounding normal tissue.


CONCLUSIONS
Although H. pylori has been implicated in pancreatic ductal adenocarcinoma, it may not play a key role in the development of intraductal papillary mucinous neoplasm.
Gastroenterology Department, Bezmialem Vakıf University, Istanbul, Turkey.
Birol
B
Baysal
Gastroenterology Department, Bezmialem Vakıf University, Istanbul, Turkey. Electronic address: dralince@gmail.com.
Ali Tüzün
AT
İnce
Microbiology Division, Bezmialem Vakıf University, Istanbul, Turkey.
Bilge
B
Gültepe
Pathology Division, Bezmialem Vakıf University, Istanbul, Turkey.
Zuhal
Z
Gücin
Surgery Department, Bezmialem Vakıf University, Istanbul, Turkey.
Fatma Ümit
FÜ
Malya
Gastroenterology Department, Bezmialem Vakıf University, Istanbul, Turkey.
Mukaddes
M
Tozlu
Gastroenterology Department, Bezmialem Vakıf University, Istanbul, Turkey.
Hakan
H
Şentürk
Pathology Division, Marmara University, Istanbul, Turkey.
Pelin
P
Bağcı
Pathology Division, Marmara University, Istanbul, Turkey.
Çiğdem Ataizi
ÇA
Çelikel
Pathology Division, Haydarpaşa Numune Education and Research Hospital, Istanbul, Turkey.
Fügen
F
Aker
Pathology Division, Haydarpaşa Numune Education and Research Hospital, Istanbul, Turkey.
Selvinaz
S
Özkara
Pathology Division, İstanbul Education and Research Hospital, Istanbul, Turkey.
Esra
E
Paşaoğlu
Pathology Division, İstanbul Education and Research Hospital, Istanbul, Turkey.
Nevra
N
Dursun
Pathology Division, Şişli Etfal Education and Research Hospital, Istanbul, Turkey.
Banu Yılmaz
BY
Özgüven
Pathology Division, Şişli Etfal Education and Research Hospital, Istanbul, Turkey.
Deniz
D
Tunçel
D002288:Adenocarcinoma, Mucinous / Q000382:microbiology*; D000231:Adenocarcinoma, Papillary / Q000382:microbiology*; D000328:Adult; D000368:Aged; D021441:Carcinoma, Pancreatic Ductal / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D016480:Helicobacter pylori; D006801:Humans; D008297:Male; D008875:Middle Aged; D010183:Pancreatic Ducts / Q000382:microbiology; D010190:Pancreatic Neoplasms / Q000382:microbiology*; D016612:Paraffin Embedding; D012189:Retrospective Studies; D012307:Risk Factors; D016707:Tissue Fixation
D004269:DNA, Bacterial
Hpylori; Intraductal papillary mucinous neoplasm; Pancreatic ductal adenocarcinoma