In vivo Monitoring of Serotonin by Nanomaterial Functionalized Acupuncture Needle.
Acupuncture treatment is amazing but controversial. Up to now, the mechanism of treating diseases by acupuncture and moxibustion is still unclear, especially the occurrence of the molecular events in local acupoints. Herein, we report an extremely stable microsensor by modifying carbon nanotube (CNT) to the tip surface of acupuncture needle and applying this CNT-modified acupuncture needle for real time monitoring of serotonin (5-HT) in vivo. To stabilize CNT modification on the needle tip surface, poly(3,4-ethylenedioxythiophene)(PEDOT) was employed as glue water to stick CNT on the needle. The detection limit of the CNT-modified needle was found to be approximately 50 nM and 78 nM in the PBS and the cell medium, respectively. In addition, the needle showed good selectivity to some inflammatory mediators and some electroactive molecules. For the first time, the CNT-modified needle could be directly probed into rat body for real time monitoring of 5-HT in vivo, showing a great potential for better understanding the mechanism of acupuncture treatment.
School of Laboratory Medicine, Hubei University of Chinese, 1 Huangjia Lake West Road, Wuhan 430065, China.
Yu-Tao
YT
Li
School of Laboratory Medicine, Hubei University of Chinese, 1 Huangjia Lake West Road, Wuhan 430065, China.
Li-Na
LN
Tang
School of Laboratory Medicine, Hubei University of Chinese, 1 Huangjia Lake West Road, Wuhan 430065, China.
Yong
Y
Ning
Hubei Provincial Collaborative Innovation Center of Preventive Treatment by Acupuncture and Moxibustion, 1 Huangjia Lake West Road, Wuhan 430065, China.
Qing
Q
Shu
Hubei Provincial Collaborative Innovation Center of Preventive Treatment by Acupuncture and Moxibustion, 1 Huangjia Lake West Road, Wuhan 430065, China.
Feng-Xia
FX
Liang
Hubei Provincial Collaborative Innovation Center of Preventive Treatment by Acupuncture and Moxibustion, 1 Huangjia Lake West Road, Wuhan 430065, China.
Hua
H
Wang
School of Laboratory Medicine, Hubei University of Chinese, 1 Huangjia Lake West Road, Wuhan 430065, China.|Hubei Provincial Collaborative Innovation Center of Preventive Treatment by Acupuncture and Moxibustion, 1 Huangjia Lake West Road, Wuhan 430065, China.
Guo-Jun
GJ
Zhang
D015670:Acupuncture Therapy / Q000295:instrumentation*; D000818:Animals; D009071:Moxibustion; D037742:Nanotubes, Carbon / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000032:analysis*
D037742:Nanotubes, Carbon; D012701:Serotonin