Anti-MRSA Activities of Enterocins DD28 and DD93 and Evidences on Their Role in the Inhibition of Biofilm Formation.
Methicillin-resistant Staphylococcus aureus (MRSA) has become a worrisome superbug. This work aimed at studying the effects of two class IIb bacteriocins, enterocins DD28 and DD93 as anti-MRSA agents. Thus, these bacteriocins were purified, from the cultures supernatants of Enterococcus faecalis 28 and 93, using a simplified purification procedure consisting in a cation exchange chromatography and a reversed-phase high-performance liquid chromatography. The anti-Staphylococcal activity was shown in vitro by the assessment of the minimal inhibitory concentration (MIC), followed by a checkerboard and time-kill kinetics experiments. The data unveiled a clear synergistic effect of enterocins DD28 and DD93 in combination with erythromycin or kanamycin against the clinical MRSA-S1 strain. Besides, these combinations impeded as well the MRSA-S1 clinical strain to setup biofilms on stainless steel and glace devices.
Université de Lille 1 Sciences et Technologies - Institut Charles Viollette Lille, France.
Ahmed K
AK
Al Atya
Université de Lille 1 Sciences et Technologies - Institut Charles Viollette Lille, France.
Yanath
Y
Belguesmia
Université de Lille 1 Sciences et Technologies - Institut Charles Viollette Lille, France.
Gabrielle
G
Chataigne
Université de Lille 1 Sciences et Technologies - Institut Charles Viollette Lille, France.
Rozenn
R
Ravallec
Hôpital Victor Provo de Roubaix Roubaix, France.
Anne
A
Vachée
Institut d'Electronique, de Microélectronique et de Nanotechnologie, UMR CNRS 8520, Université Lille 1 Lille, France.
Sabine
S
Szunerits
Institut d'Electronique, de Microélectronique et de Nanotechnologie, UMR CNRS 8520, Université Lille 1 Lille, France.
Rabah
R
Boukherroub
Université de Lille 1 Sciences et Technologies - Institut Charles Viollette Lille, France.
Djamel
D
Drider
MRSA; antibiotics; bacteriocins; enterocins; synergism