Mycobacterium tuberculosis High-Throughput Screening.
High-throughput screening is a valuable way to identify hit compounds that combined with a robust medicinal chemistry program could lead to the identification of new antibiotics. Here, we discuss our method for screening large compound libraries with virulent Mycobacterium tuberculosis, possibly one of the more difficult bacteria to use because of its slow growth and assignment to Biosafety Level-3 by the CDC and NIH. The principles illuminated here, however, are relevant to the execution of most bacteria high-throughput screens.
High Throughput Screening Center, Southern Research Institute, Birmingham, AL, USA. lwhite@southernresearch.org.
E Lucile
EL
White
High Throughput Screening Center, Southern Research Institute, Birmingham, AL, USA.
Nichole A
NA
Tower
High Throughput Screening Center, Southern Research Institute, Birmingham, AL, USA.
Lynn
L
Rasmussen
D000818:Animals; D000995:Antitubercular Agents / Q000494:pharmacology*; D018929:Cell Culture Techniques / Q000379:methods; D002522:Chlorocebus aethiops; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000254:growth & development; D054852:Small Molecule Libraries / Q000494:pharmacology; D014376:Tuberculosis / Q000188:drug therapy* / Q000382:microbiology; D014709:Vero Cells
D000995:Antitubercular Agents; D054852:Small Molecule Libraries
Antibacterial; Antibiotics; Antimicrobial; Antitubercular; H37Rv; HTS; High-throughput screening; Mtb; Mycobacterium tuberculosis; TB; Tuberculosis