Full spectrum and selected spectrum based multivariate calibration methods for simultaneous determination of betamethasone dipropionate, clotrimazole and benzyl alcohol: Development, validation and application on commercial dosage form.
Five different chemometric methods were developed for the simultaneous determination of betamethasone dipropionate (BMD), clotrimazole (CT) and benzyl alcohol (BA) in their combined dosage form (Lotriderm® cream). The applied methods included three full spectrum based chemometric techniques; namely principal component regression (PCR), Partial Least Squares (PLS) and Artificial Neural Networks (ANN), while the other two methods were PLS and ANN preceded by genetic algorithm procedure (GA-PLS and GA-ANN) as a wavelength selection procedure. A multilevel multifactor experimental design was adopted for proper construction of the models. A validation set composed of 12 mixtures containing different ratios of the three analytes was used to evaluate the predictive power of the suggested models. All the proposed methods except ANN, were successfully applied for the analysis of their pharmaceutical formulation (Lotriderm® cream). Results demonstrated the efficiency of the four methods as quantitative tool for analysis of the three analytes without prior separation procedures and without any interference from the co-formulated excipient. Additionally, the work highlighted the effect of GA on increasing the predictive power of PLS and ANN models.
Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia; Department of Analytical Chemistry, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, 11562 Cairo, Egypt. Electronic address: hdarwish75@yahoo.com.
Hany W
HW
Darwish
Department of Analytical Chemistry, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, 11562 Cairo, Egypt. Electronic address: eman.elzanfaly@gmail.com.
Eman S
ES
Elzanfaly
Department of Analytical Chemistry, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, 11562 Cairo, Egypt. Electronic address: ahmedss_pharm@yahoo.com.
Ahmed S
AS
Saad
Department of Analytical Chemistry, Faculty of Pharmacy, Cairo University, Kasr El-Aini Street, 11562 Cairo, Egypt. Electronic address: ommoaz82@yahoo.com.
Abdelaziz El-Bayoumi
Ael-B
Abdelaleem
D000465:Algorithms; D000779:Anesthetics, Local / Q000032:analysis*; D000891:Anti-Infective Agents, Local / Q000032:analysis*; D000893:Anti-Inflammatory Agents / Q000032:analysis*; D019905:Benzyl Alcohol / Q000032:analysis*; D001623:Betamethasone / Q000031:analogs & derivatives* / Q000032:analysis; D002138:Calibration; D003022:Clotrimazole / Q000032:analysis*; D004338:Drug Combinations; D016018:Least-Squares Analysis; D016571:Neural Networks, Computer; D025341:Principal Component Analysis; D063465:Skin Cream / Q000032:analysis; D013053:Spectrophotometry / Q000379:methods
D000779:Anesthetics, Local; D000891:Anti-Infective Agents, Local; D000893:Anti-Inflammatory Agents; D004338:Drug Combinations; C011175:betamethasone-17,21-dipropionate; D001623:Betamethasone; D003022:Clotrimazole; D019905:Benzyl Alcohol
ANN; Benzyl alcohol; Betamethasone dipropionate; Clotrimazole; GA; PCR; PLS