Qualitative and quantitative analysis of ochratoxin A contamination in green coffee beans using Fourier transform near infrared spectroscopy.
BACKGROUND
Ochratoxin A (OTA) contamination is highly prevalent in a variety of agricultural products including the commercially important coffee bean. As such, rapid and accurate detection methods are considered necessary for the identification of OTA in green coffee beans. The goal of this research was to apply Fourier transform near infrared spectroscopy to detect and classify OTA contamination in green coffee beans in both a quantitative and qualitative manner.


RESULTS
PLSR models were generated using pretreated spectroscopic data to predict the OTA concentration. The best model displayed a correlation coefficient (r) of 0.814, a standard error of prediction (SEP and bias of 1.965 µg kg-1and 0.358 µg kg-1, respectively. Additionally, a PLS-DA model was also generated, displaying a classification accuracy of 96.83% for a non-OTA contaminated model and 80.95% for an OTA contaminated model, with an overall classification accuracy of 88.89%.


CONCLUSIONS
The results demonstrate that the developed model could be used for detecting OTA contamination in green coffee beans in either a quantitative or qualitative manner. © 2016 Society of Chemical Industry.
Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand.
Panchita
P
Taradolsirithitikul
Curriculum of Agricultural Engineering, Department of Mechanical Engineering, Faculty of Engineering, King Mongkut's Institute of Technology Ladkrabang, Bangkok, 10520, Thailand.
Panmanas
P
Sirisomboon
Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand.
Cheewanun
C
Dachoupakan Sirisomboon
D040503:Coffea; D003069:Coffee / Q000737:chemistry*; D005506:Food Contamination / Q000032:analysis*; D006801:Humans; D015233:Models, Statistical; D009793:Ochratoxins / Q000032:analysis*; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D019265:Spectroscopy, Near-Infrared
D003069:Coffee; D009793:Ochratoxins; C025589:ochratoxin A
Fourier transform near infrared spectroscopy; green coffee beans; ochratoxin A; partial least square discriminant analysis (PLS-DA); partial least square regression (PLSR)