Development and characterization of microsatellite markers for Ulmus chenmoui (Ulmaceae), an endangered tree endemic to eastern China.
Ulmus chenmoui (Ulmaceae) is an endangered tree found on Langya Mountain, eastern China. To better understand the population genetics of U. chenmoui and conserve the species, we developed microsatellite markers. Using a suppression-polymerase chain reaction technique, 74 compound microsatellite primer pairs were designed. Twelve microsatellite markers were polymorphic in 39 individuals, and the number of alleles per locus ranged from 3 to 9. The observed and expected heterozygosities ranged from 0.051 to 0.769 and from 0.533 to 0.768, respectively. Significant linkage disequilibrium was detected for three pairs of loci (P < 0.01), which may be due to a recent population bottleneck and the small population size. Nine of the 12 loci deviated from the Hardy-Weinberg equilibrium (P < 0.01), which could be explained by significant inbreeding rather than the presence of null alleles. These markers will provide a solid basis for future efforts in population genetic studies of U. chenmoui, which in turn will contribute to species conservation.
School of Life Science, Nanjing University, Nanjing, Jiangsu, China.|State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing, Jiangsu, China.
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Geng
School of Life Science, Nanjing University, Nanjing, Jiangsu, China.|State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing, Jiangsu, China.
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He
School of Life Science, Nanjing University, Nanjing, Jiangsu, China.|State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing, Jiangsu, China.
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Yang
School of Life Science, Nanjing University, Nanjing, Jiangsu, China.|State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing, Jiangsu, China.
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Shi
School of Life Science, Nanjing University, Nanjing, Jiangsu, China.|State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing, Jiangsu, China.
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Wang
School of Life Science, Nanjing University, Nanjing, Jiangsu, China.|State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing, Jiangsu, China.
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Xu
School of Life Science, Nanjing University, Nanjing, Jiangsu, China.|State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing, Jiangsu, China.
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Jeelani
School of Life Science, Nanjing University, Nanjing, Jiangsu, China.|State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing, Jiangsu, China.
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Wang
Department of Earth and Environment, International Center for Tropical Botany, Florida International University, Miami, FL, USA.|College of Forestry, Guangxi University, Nanning, China.
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Liu
D000483:Alleles; D002681:China; D003247:Conservation of Natural Resources; D056426:Genetic Loci; D005828:Genetics, Population; D015810:Linkage Disequilibrium; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D014197:Trees / Q000235:genetics*; D034403:Ulmus / Q000235:genetics*