Overexpression of soybean R2R3-MYB transcription factor, GmMYB12B2, and tolerance to UV radiation and salt stress in transgenic Arabidopsis.
MYB, v-myb avian myeloblastosis viral oncogene homolog, proteins play central roles in plant stress response. Previously, we identified a novel R2R3-MYB transcription factor, GmMYB12B2, which affected the expression levels of some key enzyme genes involved in flavonoid biosynthesis in transgenic Arabidopsis. In the present study, we analyzed the expression levels of GmMYB12B2 under salt, low temperature, drought, abscisic acid (ABA), and ultraviolet (UV) radiation treatments in soybean using semi-quantitative reverse transcription polymerase chain reaction. The expression of GmMYB12B2 was drastically induced by UV irradiation and salt treatment, but no response was detected under low temperature, drought, and ABA stresses. A detailed characterization of the GmMYB12B2 overexpression lines revealed that GmMYB12B2 might be involved in response of plants to UV radiation and salt stresses. Transgenic Arabidopsis lines constitutively expressing GmMYB12B2 showed an increased tolerance to salt and UV radiation treatment compared with wild-type plants. The expression levels of certain salt stress-responsive genes, such as DREB2A and RD17, were found to be elevated in the transgenic plants. These results indicate that GmMYB12B2 acts as a regulator in the plant stress response.
College of Plant Science, Jilin University, Changchun, China.|College of Life Science, Engineering Research Center of the Chinese Ministry of Education for Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, Jilin, China.
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Li
College of Plant Science, Jilin University, Changchun, China.
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Wang
College of Plant Science, Jilin University, Changchun, China.
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Yan
College of Plant Science, Jilin University, Changchun, China.
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Li
College of Life Science and Agroforestry, Qiqihaer University, Qiqihaer, China.
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Zhao
Jilin Province Institute of Product Quality Supervision and Inspection, Changchun, China.
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Zhao
College of Life Science and Agroforestry, Qiqihaer University, Qiqihaer, China.
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Zhai
College of Plant Science, Jilin University, Changchun, China.
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Wang
D000040:Abscisic Acid / Q000235:genetics; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics; D029681:Arabidopsis Proteins / Q000096:biosynthesis / Q000235:genetics*; D003080:Cold Temperature; D055864:Droughts; D005419:Flavonoids / Q000096:biosynthesis / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000528:radiation effects; D030821:Plants, Genetically Modified / Q000235:genetics / Q000528:radiation effects; D012965:Sodium Chloride / Q000633:toxicity; D013025:Glycine max / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D013312:Stress, Physiological / Q000235:genetics*; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D014466:Ultraviolet Rays
D029681:Arabidopsis Proteins; C482738:BOTRYTIS SUSCEPTIBLE1 protein, Arabidopsis; C114579:DREB2A protein, Arabidopsis; D005419:Flavonoids; D014157:Transcription Factors; D012965:Sodium Chloride; D000040:Abscisic Acid