Brief Report: A High Rate of β7+ Gut-Homing Lymphocytes in HIV-Infected Immunological Nonresponders is Associated With Poor CD4 T-Cell Recovery During Suppressive HAART.
Correlation between GALT homing markers on lymphocytes and the low blood CD4 T-cell reconstitution in immunological nonresponders (INRs) has been studied.



Thirty-one INRs, 19 immunological responders (IRs), and 12 noninfected controls were enrolled in this study. INRs were defined by an undetectable plasma viral load RNA less than 40 copies per milliliter and CD4 T-cell count <500 cells per cubic milliliter in at least 3 years.



A complete peripheral and mucosal lymphocyte immunophenotyping was performed on these patients with a focus on the CCR9, CCR6, and α4β7 gut-homing markers.



A highly significant upregulation of α4β7 on INRs peripheral lymphocytes compared with that of IRs has been observed. This upregulation impacts different lymphocyte subsets namely CD4, CD8, and B lymphocytes. The frequency of β7 Th17 and Treg cells are increased compared with IRs and healthy controls. The frequency of β7 CD8 T cells in the blood is negatively correlated with integrated proviral DNA in rectal lymphoid cells in contrast to β7 CD4 T cells associated with HIV integration.



Alteration of lymphocyte homing abilities would have deleterious effects on GALT reconstitution and could participate to HIV reservoir constitution. These results emphasize the great interest to consider α4β7-targeted therapy in INR patients to block homing of lymphocytes and/or to directly impair gp120-α4β7 interactions.
*Groupe Immunité des Muqueuses et Agents Pathogènes, Université de Saint-Etienne, Université de Lyon; †Service de Maladies Infectieuses et Tropicales; ‡Laboratoire d'Immunologie; §Service d'Hépato-gastroentérologie; and ‖Laboratoire de Virologie, Centre Hospitalo-Universitaire Nord, Saint-Etienne, France.
Alexandre
A
Girard
Delphine
D
Vergnon-Miszczycha
Anne-Emmanuelle
AE
Depincé-Berger
Xavier
X
Roblin
Frederic
F
Lutch
Claude
C
Lambert
Nicolas
N
Rochereau
Thomas
T
Bourlet
Christian
C
Genin
Stéphane
S
Paul
D023241:Antiretroviral Therapy, Highly Active; D001005:Anus Neoplasms / Q000276:immunology / Q000473:pathology / Q000821:virology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018450:Disease Progression; D055088:Early Detection of Cancer; D005434:Flow Cytometry; D015658:HIV Infections / Q000188:drug therapy / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D016130:Immunophenotyping; D007413:Intestinal Mucosa / Q000276:immunology / Q000473:pathology / Q000821:virology*; D012367:RNA, Viral / Q000276:immunology*; D016201:Receptors, Lymphocyte Homing / Q000187:drug effects / Q000276:immunology*; D019562:Viral Load
D012367:RNA, Viral; D016201:Receptors, Lymphocyte Homing