Correlates of Elevated Interleukin-6 and 8-Hydroxy-2'-Deoxyguanosine Levels in Tracheal Aspirates from Very Low Birth Weight Infants Who Develop Bronchopulmonary Dysplasia.
Bronchopulmonary dysplasia (BPD) remains the most common complication of very low birth weight (VLBW) preterm infants, and inflammatory regulation plays a role in the development of the BPD. Interleukin-6 (IL-6) has an important role in airway inflammation and therefore can be used as a marker of airway injury. The study aimed to compare the changes between IL-6 and oxidative stress marker with 8-hydroxy-2'-deoxyguanosine (8-OHdG) from serum and tracheal aspiration (TA) in VLBW preterm infants following development of BPD.



This birth cohort study enrolled 80 VLBW preterm infants, including 26 who developed BPD. All infants completed the study and survived at 36 weeks postmenstrual age. IL-6 and 8-OHdG concentrations from serum and TA on Day 1 and Day 28 after birth were measured using immunoassay.



IL-6 and 8-OHdG in serum and TA were higher in the BPD group than in the non-BPD group on the 1stday after birth (p < 0.05). The IL-6 and 8-OHdG levels in TA fluid were persistently increased on the 28thday of life in the BPD group (p < 0.05). The TA IL-6 was positively correlated with 8-OHdG levels on the 1stday (r = 0.64, p < 0.05) and 28thday of life (r = 0.76, p < 0.05). Based on receiver operating characteristic curves as a predictor of BPD development, TA IL-6 (cutoff, 456.8 pg/mg) had 81.5% sensitivity and 77.8% specificity, whereas TA 8-OHdG (cutoff, 4.4 ng/mg) had a sensitivity of 81.5% and a specificity of 64.4%.



Persistent inflammation with oxidative DNA damage in the respiratory tract may be a crucial mechanism in BPD.
Department of Pediatrics, Changhua Christian Children's Hospital, Changhua City, Taiwan; School of Medicine, Kaohsiung Medical University, Kaohsiung City, Taiwan.
Chien-Chou
CC
Hsiao
Vascular and Genomic Research Center, Changhua Christian Hospital, Changhua City, Taiwan.
Jui-Chih
JC
Chang
Department of Pediatrics, Changhua Christian Children's Hospital, Changhua City, Taiwan.
Lon-Yen
LY
Tsao
Department of Pediatrics, Changhua Christian Children's Hospital, Changhua City, Taiwan.
Rei-Cheng
RC
Yang
Department of Pediatrics, Changhua Christian Children's Hospital, Changhua City, Taiwan; School of Medicine, Chung Shan Medical University, Taichung City, Taiwan.
Hsiao-Neng
HN
Chen
Department of Pediatrics, Changhua Christian Children's Hospital, Changhua City, Taiwan.
Cheng-Han
CH
Lee
Department of Pediatrics, Children's Hospital, China Medical University, Taichung City, Taiwan.
Ching-Yuang
CY
Lin
Department of Pediatrics, Changhua Christian Children's Hospital, Changhua City, Taiwan; School of Medicine, Kaohsiung Medical University, Kaohsiung City, Taiwan; School of Medicine, Chung Shan Medical University, Taichung City, Taiwan. Electronic address: 107239@cch.org.tw.
Yi-Giien
YG
Tsai
D000080242:8-Hydroxy-2'-Deoxyguanosine; D015415:Biomarkers / Q000378:metabolism; D001997:Bronchopulmonary Dysplasia / Q000378:metabolism*; D015331:Cohort Studies; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007234:Infant, Premature; D019102:Infant, Very Low Birth Weight; D015850:Interleukin-6 / Q000378:metabolism*; D008297:Male; D014132:Trachea / Q000378:metabolism*
D015415:Biomarkers; C508600:IL6 protein, human; D015850:Interleukin-6; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine
8-hydroxydeoxyguanosine; bronchopulmonary dysplasia; premature infant