Developing a dermatological photodiagnosis system by optical image analyses and in vivo study.
Dermatological photodynamic therapy (DPDT) involves using systematic photosensitizers in combination with light irradiation treatment to eliminate cancer cells. Therefore, a noninvasive fluorescence photodiagnosis system is critical in DPDT for diagnosing target tissues and demarcating the margin of normal tissues. This study proposes a 375-nm ring LED light module in fluorescence imaging for DPDT applications. Image reproducibility (I.R.) and image interference (I.I.) analysis were performed. The results showed that the I.R. value of this fluorescence diagnostic system was higher than 99.0%, and the I.I. from external light sources was lower than 3.0%. In addition, the result of an in vivo study showed that the Chlorin e6 red fluorescence and the scope of distribution of B16-F10 melanoma cells in a mouse ear's vein could be measured clearly using our device; however, the comparison studio with 395-nm LED lights could not focus or capture the red fluorescence effectively.
School of Medicine, College of Medicine, China Medical University, Taichung 40402, Taiwan; Departments of Dermatology, China Medical University Hospital, Taichung 40447, Taiwan; Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan. Electronic address: miriamchangch@gmail.com.
Chung-Hsing
CH
Chang
Instrument Technology Research Center, National Applied Research Laboratories, Hsinchu 30076, Taiwan.
Yu-Hsuan
YH
Lin
Instrument Technology Research Center, National Applied Research Laboratories, Hsinchu 30076, Taiwan.
Cheng-Ru
CR
Li
Instrument Technology Research Center, National Applied Research Laboratories, Hsinchu 30076, Taiwan.
Chun-Ming
CM
Chang
Instrument Technology Research Center, National Applied Research Laboratories, Hsinchu 30076, Taiwan.
Chih-Wei
CW
Hung
Instrument Technology Research Center, National Applied Research Laboratories, Hsinchu 30076, Taiwan. Electronic address: roman@narlabs.org.tw.
Han-Chao
HC
Chang
D000818:Animals; D045744:Cell Line, Tumor; D002735:Chlorophyllides; D046169:Dermoscopy / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005456:Fluorescent Dyes; D008029:Lighting / Q000295:instrumentation / Q000379:methods; D008545:Melanoma / Q000000981:diagnostic imaging* / Q000473:pathology*; D051379:Mice; D008819:Mice, Nude; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D010781:Photography / Q000295:instrumentation; D011166:Porphyrins; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012878:Skin Neoplasms / Q000000981:diagnostic imaging* / Q000473:pathology*
D002735:Chlorophyllides; D005456:Fluorescent Dyes; D011166:Porphyrins; C062985:phytochlorin
375-nm; Chlorin (e6); Image interference; Image reproducibility; Photodiagnosis system; Photodynamic therapy