A New Electrolytic Synthesis Method for Few-Layered MoS2 Nanosheets and Their Robust Biointerfacing with Reduced Antibodies.
We report an efficient method for the synthesis of few-layered MoS2 nanosheets and demonstrate their application in the label-free detection of the prostate-specific antigen (PSA) cancer marker. As a novel strategy, the electro-dissolution of molybdenum metal sheets in the presence of Na(+) and S(2-) ions led to the formation of Na(+) intercalated MoS2. Further exfoliation by ultrasonication yielded the desired formation of few-layered MoS2 nanosheets. After comprehensive characterization, the synthesized MoS2 nanosheets were channeled in a field-effect transistor (FET) microdevice. Chemically reduced anti-PSA antibodies were immobilized on the MoS2 channel above the FET microdevice to construct a specific PSA immunosensor. The antibodies were deliberately reduced to expose the hinge-region disulfide bonds. This approach offered a robust and site-directed immunosensing device through biointerfacing of the sulfhydryl groups (-SH) in the reduced antibody with the surface S atoms of MoS2. This device was validated as an effective immunosensor with a low detection limit (10(-5) ng/mL) over a wide linear detection range (10(-5) to 75 ng/mL).
CSIR-Central Scientific Instrument Organisation (CSIR-CSIO) , Chandigarh 160030, India.|Academy of Scientific and Innovative Research (AcSIR-CSIO) , Chandigarh 160030, India.
Manil
M
Kukkar
CSIR-Central Scientific Instrument Organisation (CSIR-CSIO) , Chandigarh 160030, India.|Academy of Scientific and Innovative Research (AcSIR-CSIO) , Chandigarh 160030, India.
Satish K
SK
Tuteja
CSIR-Central Scientific Instrument Organisation (CSIR-CSIO) , Chandigarh 160030, India.
Amit L
AL
Sharma
Icon Analytical Equipment (P) Ltd., Mumbai 400018, India.
Vinod
V
Kumar
CSIR-Central Scientific Instrument Organisation (CSIR-CSIO) , Chandigarh 160030, India.
Ashok K
AK
Paul
Department of Civil and Environmental Engineering, Hanyang University , 222 Wangsimni-Ro, Seoul 04763, Korea.
Ki-Hyun
KH
Kim
Institute of Nano Science & Technology , Mohali 160062, India.
Priyanka
P
Sabherwal
CSIR-Central Scientific Instrument Organisation (CSIR-CSIO) , Chandigarh 160030, India.|Academy of Scientific and Innovative Research (AcSIR-CSIO) , Chandigarh 160030, India.
Akash
A
Deep
D000906:Antibodies / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000032:analysis / Q000378:metabolism; D003950:Diagnostic Techniques, Urological / Q000295:instrumentation*; D006801:Humans; D057230:Limit of Detection; D008297:Male; D008670:Metals / Q000737:chemistry; D008982:Molybdenum / Q000737:chemistry; D049329:Nanostructures; D017430:Prostate-Specific Antigen / Q000032:analysis; D015203:Reproducibility of Results
D000906:Antibodies; D014408:Biomarkers, Tumor; D008670:Metals; D008982:Molybdenum; D017430:Prostate-Specific Antigen
FET; MoS2; few-layered nanosheets; immunosensor; prostate-specific antigen; reduced antibody