Extraction and Characterization of Tamarind (Tamarind indica L.) Seed Polysaccharides (TSP) from Three Difference Sources.
Tamarind seed polysaccharide (TSP), a natural polysaccharide extracted from tamarind seeds is used in the pharmaceutical, textile and food industries as a mucoadhesive polymer. This work aimed to extract TSP from tamarind seeds from three sources with two methods and characterized its physical and chemical properties. Kernel powder of tamarind seeds was slurried into a clear solution, set aside overnight and then centrifuged at 6000 rpm for 20 min to separate all foreign matter. The supernatant was separated and poured into excess 95% ethanol with continuous stirring. The precipitate obtained was collected and dried in the oven and then the dried TSP polymer was stored in a desiccator. The dried TSP was analyzed by ¹H-NMR, FT-IR and XRD. The results showed TSP from tamarind seeds taken from paddy farmland (A), a waste from the export tamarind juice industry (B) and the export tamarind powder industry(C) gave yields of 31.55%, 26.95% and 17.30%, respectively, using method 1 and 11.15%, 53.65% and 54.65%, with method 2, respectively, but method 2 gave purer TSP than method 1. The FT-IR spectra displayed peaks at 3351.95 cm(-1), 2920.76 cm(-1), 1018.85 cm(-1) and 555.16 cm(-1). The ¹H-NMR showed polysaccharide peaks between δ 3.50-4.20 ppm and XRD diagrams indicated their amorphous nature. Future works will focus on the quantitative analysis, biological activity and possible use of TSP as a drug delivery system.
Thailand Institute of Scientific and Technological Research (TISTR), 35 Mu 3 Tambon Khlong Ha, Amphoe Khlong Luang, Pathum Thani 12120, Thailand. khanitha@tistr.or.th.
Khanittha
K
Chawananorasest
Thailand Institute of Scientific and Technological Research (TISTR), 35 Mu 3 Tambon Khlong Ha, Amphoe Khlong Luang, Pathum Thani 12120, Thailand. new_ninsho@hotmail.com.
Patsuda
P
Saengtongdee
Thailand Institute of Scientific and Technological Research (TISTR), 35 Mu 3 Tambon Khlong Ha, Amphoe Khlong Luang, Pathum Thani 12120, Thailand. praphakorn@tistr.or.th.
Praphakorn
P
Kaemchantuek
D016503:Drug Delivery Systems; D011108:Polymers / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D066244:Proton Magnetic Resonance Spectroscopy; D012639:Seeds / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D029861:Tamarindus / Q000737:chemistry*
D011108:Polymers; D011134:Polysaccharides
Tamarind indica L.; natural polysaccharides; tamarind seed polysaccharide (TSP)