Determination of 13 Organic Toxicants in Human Blood by Liquid-Liquid Extraction Coupling High-Performance Liquid Chromatography Tandem Mass Spectrometry.
Pesticides and antidepressants are frequently misused in drug-facilitated crime because of their toxicological effect and easy-availability. Therefore, it is essential for the development of a simple and reliable method for the determination of these organic toxicants in biological fluids. Here, we report on an applicable method by the combination of optimized liquid-liquid extraction (LLE) procedure and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) to identify and quantify dimethoate, omethoate, dichlorvos, carbofuran, fenpropathrin, diazepam, estazolam, alprazolam, triazolamm, chlorpromazine, phenergan, barbitone and phenobarbital in human blood. The method demonstrated a linear calibration curve in range of 20 - 500 μg/L (r > 0.994). The accuracy evaluated by recovery spiked at three different concentrations (50, 100 and 200 μg/L) was in the range of 58.8 - 83.1% with a relative standard deviations (RSD) of 3.7 - 7.4%. The limits of quantification ranged over 6.7 - 33.3 μg/L. This method was proved to be simple and reliable, and was thus successfully applied to forensic toxicology.
School of Public Security & Technology, Gansu Institute of Political Science and Law.
Aiying
A
Song
D000928:Antidepressive Agents / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification*; D001774:Blood Chemical Analysis / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D016014:Linear Models; D059625:Liquid-Liquid Extraction / Q000379:methods*; D010575:Pesticides / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification*; D012997:Solvents / Q000737:chemistry; D053719:Tandem Mass Spectrometry
D000928:Antidepressive Agents; D010575:Pesticides; D012997:Solvents