Rhus javanica Gall Extract Inhibits the Differentiation of Bone Marrow-Derived Osteoclasts and Ovariectomy-Induced Bone Loss.
Inhibition of osteoclast differentiation and bone resorption is a therapeutic strategy for the management of postmenopausal bone loss. This study investigated the effects of Rhus javanica (R. javanica) extracts on bone marrow cultures to develop agents from natural sources that may prevent osteoclastogenesis. Extracts of R. javanica (eGr) cocoons spun by Rhus javanica (Bell.) Baker inhibited the osteoclast differentiation and bone resorption. The effects of aqueous extract (aeGr) or 100% ethanolic extract (eeGr) on ovariectomy- (OVX-) induced bone loss were investigated by various biochemical assays. Furthermore, microcomputed tomography (µCT) was performed to study bone remodeling. Oral administration of eGr (30 mg or 100 mg/kg/day for 6 weeks) augmented the inhibition of femoral bone mineral density (BMD), bone mineral content (BMC), and other factors involved in bone remodeling when compared to OVX controls. Additionally, eGr slightly decreased bone turnover markers that were increased by OVX. Therefore, it may be suggested that the protective effects of eGr could have originated from the suppression of OVX-induced increase in bone turnover. Collectively, the findings of this study indicate that eGr has potential to activate bone remodeling by inhibiting osteoclast differentiation and bone loss.
Biomedical Research Institute, Kyungpook National University Hospital, Daegu 41940, Republic of Korea; Skeletal Diseases Genome Research Center, Kyungpook National University Hospital, Daegu 41940, Republic of Korea.
Tae-Ho
TH
Kim
Department of Oral Pathology and Regenerative Medicine, School of Dentistry, IHBR, Kyungpook National University, Daegu 41940, Republic of Korea.
Eui Kyun
EK
Park
Department of Ophthalmology, Graduate School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.
Man-Il
MI
Huh
Department of Ophthalmology, Graduate School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.
Hong Kyun
HK
Kim
Department of Orthopedic Surgery, Graduate School of Medicine, Kyungpook National University, Daegu 41944, Republic of Korea.
Shin-Yoon
SY
Kim
School of Food Science & Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea.
Sang-Han
SH
Lee