MiR-932 Regulates Pyrethroid Resistance in Culex pipiens pallens (Diptera: Culicidae).
MicroRNAs (miRNAs) play notable role in regulation of gene expression at the posttranscription level, and have been involved in many biological processes, including insecticide resistance. In this study, we investigated the role of miR-932 in the molecular mechanisms of pyrethroid resistance in Culex pipiens pallens (L.). Overexpression of miR-932 in the DS-strain made the mosquitoes more resistant to deltamethrin, while inhibiting the expression of miR-932 in the DR-strain made the mosquitoes more sensitive to deltamethrin. Further, we also identified CpCPR5 as a target gene of miR-932. Sustained overexpression of miR-932 resulted in repression of CpCPR5, and that knockdown of miR-932 increased CpCPR5 expression. In addition, knockdown of CpCPR5 decreased the sensitivity of mosquitoes to deltamethrin in the DS-strain. In conclusion, our study finds a molecular link between miR-932 and CpCPR5 and provides a novel insight into the mechanism of insecticide resistance.
Department of Pathogen Biology, Nanjing Medical University, 140 Hanzhong Rd., Nanjing, Jiangsu 210029, China (fuandniu@126.com; tmm0201@126.com; guoqin2782@163.com; malei@njmu.edu.cn; zhoudan@njmu.edu.cn; shenbo@njmu.edu.cn; sunyan@njmu.edu.cn; clzhu@njmu.edu.cn).
Bingqian
B
Liu
Department of Pathogen Biology, Nanjing Medical University, 140 Hanzhong Rd., Nanjing, Jiangsu 210029, China (fuandniu@126.com; tmm0201@126.com; guoqin2782@163.com; malei@njmu.edu.cn; zhoudan@njmu.edu.cn; shenbo@njmu.edu.cn; sunyan@njmu.edu.cn; clzhu@njmu.edu.cn).
Mengmeng
M
Tian
Department of Pathogen Biology, Nanjing Medical University, 140 Hanzhong Rd., Nanjing, Jiangsu 210029, China (fuandniu@126.com; tmm0201@126.com; guoqin2782@163.com; malei@njmu.edu.cn; zhoudan@njmu.edu.cn; shenbo@njmu.edu.cn; sunyan@njmu.edu.cn; clzhu@njmu.edu.cn).
Qin
Q
Guo
Department of Pathogen Biology, Nanjing Medical University, 140 Hanzhong Rd., Nanjing, Jiangsu 210029, China (fuandniu@126.com; tmm0201@126.com; guoqin2782@163.com; malei@njmu.edu.cn; zhoudan@njmu.edu.cn; shenbo@njmu.edu.cn; sunyan@njmu.edu.cn; clzhu@njmu.edu.cn).
Lei
L
Ma
Department of Pathogen Biology, Nanjing Medical University, 140 Hanzhong Rd., Nanjing, Jiangsu 210029, China (fuandniu@126.com; tmm0201@126.com; guoqin2782@163.com; malei@njmu.edu.cn; zhoudan@njmu.edu.cn; shenbo@njmu.edu.cn; sunyan@njmu.edu.cn; clzhu@njmu.edu.cn).
Dan
D
Zhou
Department of Pathogen Biology, Nanjing Medical University, 140 Hanzhong Rd., Nanjing, Jiangsu 210029, China (fuandniu@126.com; tmm0201@126.com; guoqin2782@163.com; malei@njmu.edu.cn; zhoudan@njmu.edu.cn; shenbo@njmu.edu.cn; sunyan@njmu.edu.cn; clzhu@njmu.edu.cn).
Bo
B
Shen
Department of Pathogen Biology, Nanjing Medical University, 140 Hanzhong Rd., Nanjing, Jiangsu 210029, China (fuandniu@126.com; tmm0201@126.com; guoqin2782@163.com; malei@njmu.edu.cn; zhoudan@njmu.edu.cn; shenbo@njmu.edu.cn; sunyan@njmu.edu.cn; clzhu@njmu.edu.cn).
Yan
Y
Sun
Department of Pathogen Biology, Nanjing Medical University, 140 Hanzhong Rd., Nanjing, Jiangsu 210029, China (fuandniu@126.com; tmm0201@126.com; guoqin2782@163.com; malei@njmu.edu.cn; zhoudan@njmu.edu.cn; shenbo@njmu.edu.cn; sunyan@njmu.edu.cn; clzhu@njmu.edu.cn).
Changliang
C
Zhu

          CpCPR5
        ; 
          Culex pipiens pallens
        ; microRNA; pyrethroid