Constitutive expression of cytochrome P450 in foetal and adult porcine livers-Effects of body weight.
The liver hosts a great number of enzymatically driven processes, including detoxification. The super-family of enzymes named cytochrome P450 (CYP) is the major participant in that process. The expression of CYPs is affected by several factors including life-stage (foetal vs. adult). In the present study we investigated the impact of birth-weight (high or low birth weight) and life-stage on constitutive expression of porcine hepatic CYP1A1, CYP1A2, CYP2A19, CYP2B22, CYP2C33, CYP2D25, CYP2E1 and CYP3A29, as well as the transcription factors controlling their expression; aryl hydrocarbon receptor, constitutive androstane receptor, pregnane X receptor, C/EBP and hepatocyte nuclear factors 1 and 4. Both RT-PCR and western blotting showed a marked increase in the expression of the adult pigs compared with prenatal pigs. Moreover, CYP2E1 mRNA expression was 7.5 fold higher in foetuses with low birth weight compared with foetuses with high birth weight. Gender did not affect the mRNA expression within the different life-stages. These results indicate a similarity to what is observed in humans and porcine foetuses may therefore be a model for humans when studying expression of CYPs.
Department of Food Science, Aarhus University, Denmark. Electronic address: Martink.rasmussen@food.au.dk.
Martin Krøyer
MK
Rasmussen
Department of Animal Science, Aarhus University, Denmark.
Peter Kappel
PK
Theil
Department of Food Science, Aarhus University, Denmark.
Niels
N
Oksbjerg
D000818:Animals; D001724:Birth Weight; D015153:Blotting, Western / Q000662:veterinary; D003433:Crosses, Genetic; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism*; D005260:Female; D047109:Fetal Development; D005333:Fetus / Q000201:enzymology* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D008099:Liver / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012727:Sex Characteristics; D034421:Sus scrofa; D015430:Weight Gain
D012333:RNA, Messenger; D003577:Cytochrome P-450 Enzyme System; D019392:Cytochrome P-450 CYP2E1
Age; Birth-Weight; CYP; Life-stage; Microsomes; Ontogeny; qPCR