A novel chemiluminescent immunoassay for detection of Toxoplasma gondii IgG in human sera.
This study describes Toxoplasma gondii IgG chemiluminescent immunoassay (CLIA) based on the use of a novel immunochemical reagents in the form of the conjugates of original acridinium ester (AE) labels attached to antibodies and SAG2-GRA1-ROP1L chimeric antigen and shows that this test is useful for diagnostic purposes.
Gdańsk University of Technology, Faculty of Chemistry, Department of Molecular Biotechnology and Microbiology, 11/12 G. Narutowicza Str., 80-233, Gdańsk, Poland. Electronic address: holec@pg.gda.pl.
Lucyna
L
Holec-Gąsior
Gdańsk University of Technology, Faculty of Chemistry, Department of Molecular Biotechnology and Microbiology, 11/12 G. Narutowicza Str., 80-233, Gdańsk, Poland.
Bartłomiej
B
Ferra
University of Gdańsk, Faculty of Chemistry, Department of Physical Chemistry, 63 Wita Stwosza Str., 80-308, Gdańsk, Poland.
Justyna
J
Czechowska
University of Gdańsk, Faculty of Chemistry, Department of Physical Chemistry, 63 Wita Stwosza Str., 80-308, Gdańsk, Poland.
Illia E
IE
Serdiuk
University of Gdańsk, Faculty of Chemistry, Department of Physical Chemistry, 63 Wita Stwosza Str., 80-308, Gdańsk, Poland.
Karol
K
Krzymiński
Gdańsk University of Technology, Faculty of Chemistry, Department of Molecular Biotechnology and Microbiology, 11/12 G. Narutowicza Str., 80-233, Gdańsk, Poland.
Józef
J
Kur
D000913:Antibodies, Protozoan / Q000097:blood*; D003955:Diagnostic Tests, Routine / Q000379:methods*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000097:blood*; D008163:Luminescent Measurements; D014122:Toxoplasma / Q000276:immunology*; D014123:Toxoplasmosis / Q000175:diagnosis*
D000913:Antibodies, Protozoan; D007074:Immunoglobulin G
Acridinium esters; CLIA; Chemiluminescence; Serological detection