Reproducibility of parameters of postocclusive reactive hyperemia measured by diffuse optical tomography.
The application of near-infrared spectroscopy (NIRS) to assess microvascular function has shown promising results. An important limitation when using a single source-detector pair, however, is the lack of depth sensitivity. Diffuse optical tomography (DOT) overcomes this limitation using an array of sources and detectors that allow the reconstruction of volumetric hemodynamic changes. This study compares the key parameters of postocclusive reactive hyperemia measured in the forearm using standard NIRS and DOT. We show that while the mean parameter values are similar for the two techniques, DOT achieves much better reproducibility, as measured by the intraclass correlation coefficient (ICC). We show that DOT achieves high reproducibility for muscle oxygen consumption (ICC: 0.99), time to maximal HbO2 (ICC: 0.94), maximal HbO2 (ICC: 0.99), and time to maximal HbT (ICC: 0.99). Absolute reproducibility as measured by the standard error of measurement is consistently smaller and close to zero (ideal value) across all parameters measured by DOT compared to NIRS. We conclude that DOT provides a more robust characterization of the reactive hyperemic response and show how the availability of volumetric hemodynamic changes allows the identification of areas of temporal consistency, which could help characterize more precisely the microvasculature.
University of Sheffield, Department of Automatic Control and Systems Engineering, Mappin Street, Sheffield S1 3JD, United Kingdom.
Ernesto E
EE
Vidal-Rosas
University of Sheffield, Department of Automatic Control and Systems Engineering, Mappin Street, Sheffield S1 3JD, United Kingdom.
Stephen A
SA
Billings
University of Sheffield, Department of Cardiovascular Science, Western Bank, Sheffield S10 2TF, United Kingdom.
Timothy
T
Chico
University of Sheffield, Department of Automatic Control and Systems Engineering, Mappin Street, Sheffield S1 3JD, United Kingdom.
Daniel
D
Coca
D006801:Humans; D006940:Hyperemia / Q000000981:diagnostic imaging*; D055806:Microvessels / Q000000981:diagnostic imaging*; D010101:Oxygen Consumption; D010108:Oxyhemoglobins / Q000378:metabolism; D015203:Reproducibility of Results; D019265:Spectroscopy, Near-Infrared; D041622:Tomography, Optical
D010108:Oxyhemoglobins