Copper sulfate improves pullulan production by bioconversion using whole cells of Aureobasidium pullulans as the catalyst.
The effects of mineral salts on pullulan production by bioconversion using whole cells of Aureobasidium pullulans CCTCC M 2012259 as the catalyst were investigated. Copper sulfate (CuSO4) improved pullulan production by 36.2% and 42.3% when added at the optimum concentration of 0.2mg/L to the bioconversion broth or seed medium, respectively, as compared with controls without CuSO4 addition. Pullulan production was further enhanced when CuSO4 was added to both seed medium and bioconversion broth simultaneously. In order to probe the mechanism of CuSO4 improvement, cell viability, membrane integrity, intracellular adenosine triphosphate (ATP) levels and the activities of key enzymes involved in pullulan biosynthesis were determined. As a result, CuSO4 increased the activities of key biosynthetic enzymes, maintained intracellular ATP at a higher level, and accelerated the rate of pullulan secretion, all of which contributed to improved pullulan production by bioconversion.
School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, PR China.
Dahui
D
Wang
School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, PR China.
Xiaomin
X
Ju
School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, PR China.
Gaochuan
G
Zhang
School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, PR China.
Donghua
D
Wang
School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, PR China. Electronic address: weigy@suda.edu.cn.
Gongyuan
G
Wei
D001203:Ascomycota / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D055162:Biocatalysis / Q000187:drug effects*; D001711:Biotransformation / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D019327:Copper Sulfate / Q000494:pharmacology*; D003470:Culture Media / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D005285:Fermentation / Q000187:drug effects; D005936:Glucans / Q000096:biosynthesis*; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D008903:Minerals / Q000494:pharmacology
D003470:Culture Media; D005936:Glucans; D008903:Minerals; C009109:pullulan; D019327:Copper Sulfate
Adenosine triphosphate (PubChem CID: 5957); Aureobasidium pullulans; Bioconversion; Copper sulfate; Copper sulfate (PubChem CID: 24462); Glucose (PubChem CID: 5793); Pullulan; Pullulan (PubChem CID: 3085039); Whole-cell biocatalyst