Insights on the properties of levofloxacin-adsorbed Sr- and Mg-doped calcium phosphate powders.
Several types of biodegradable materials have been investigated for the treatment of osteomyelitis. Calcium phosphate (CaP) ceramics are among the most performing materials due to their resemblance to human hard tissues in terms of mineralogical composition, and proven ability to adsorb and deliver a number of drugs. This research work was intended to study the suitability of modified CaP powders loaded with a fluoroquinolone as drug delivery systems for osteomyelitis treatment. Levofloxacin (LEV) was chosen due to the well-recognized anti-staphylococcal activity and adequate penetration into osteoarticular tissues. Substituted CaP powders (5 mol% Sr(2+) or 5 mol% Mg(2+)) were synthesised through aqueous precipitation. The obtained powders were characterised by X-ray diffraction, SEM and FTIR analysis. The X-ray diffraction patterns confirmed the presence of HA and β-tricalcium phosphates (β-TCP) phases in doped compositions, especially in the case of Mg-doped system. The fixation of LEV at the surface of the particles occurred only by physisorption. Both the in vitro microbiological susceptibility, against Staphylococcus spp, and biocompatibility of LEV-loaded CaP powders have not been compromised.
Department of Materials and Ceramics Engineering, CICECO, University of Aveiro, 3810-193, Aveiro, Portugal. acmarques@ua.pt.
Catarina F
CF
Marques
Research Institute for Medicines (iMed. ULisboa), Faculty of Pharmacy, University of Lisbon, Av. Prof. Gama Pinto, 1649-003, Lisbon, Portugal.
Ana C
AC
Matos
Research Institute for Medicines (iMed. ULisboa), Faculty of Pharmacy, University of Lisbon, Av. Prof. Gama Pinto, 1649-003, Lisbon, Portugal.
Isabel A C
IA
Ribeiro
Research Institute for Medicines (iMed. ULisboa), Faculty of Pharmacy, University of Lisbon, Av. Prof. Gama Pinto, 1649-003, Lisbon, Portugal.
Lídia M
LM
Gonçalves
Research Institute for Medicines (iMed. ULisboa), Faculty of Pharmacy, University of Lisbon, Av. Prof. Gama Pinto, 1649-003, Lisbon, Portugal.
Ana
A
Bettencourt
Department of Materials and Ceramics Engineering, CICECO, University of Aveiro, 3810-193, Aveiro, Portugal.
José M F
JM
Ferreira
D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry; D001704:Biopolymers / Q000737:chemistry; D002130:Calcium Phosphates / Q000737:chemistry*; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli; D006801:Humans; D064704:Levofloxacin / Q000737:chemistry*; D008274:Magnesium / Q000737:chemistry*; D051379:Mice; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D010019:Osteomyelitis / Q000188:drug therapy / Q000517:prevention & control; D011208:Powders / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D013210:Staphylococcus / Q000378:metabolism; D013211:Staphylococcus aureus; D013212:Staphylococcus epidermidis; D013324:Strontium / Q000737:chemistry*; D014961:X-Ray Diffraction
D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D001704:Biopolymers; D002130:Calcium Phosphates; D011208:Powders; D064704:Levofloxacin; C020243:calcium phosphate; D008274:Magnesium; D013324:Strontium