GLUT3 is present in Clone 9 liver cells and translocates to the plasma membrane in response to insulin.
Clone 9 cells have been reported to express only the GLUT1 facilitative glucose transporter; however, previous studies have not examined Clone 9 cells for GLUT3 content. The current study sought to profile the presence of glucose transporters in Clone 9 cells, H4IIE hepatoma cells, and L6 myoblasts and myotubes. While the other cell types contained the expected complement of transporters, Clone 9 cells had GLUT3 which was previously not reported. Interestingly, both GLUT3 mRNA and protein were detected in Clone 9 cells, but only mRNA for GLUT1 was detected. Glucose transport in Clone 9 cells was insulin-sensitive in a concentration-dependent manner, concomitant with the presence of GLUT3 in the plasma membrane after insulin treatment. Although basal glucose uptake was unaffected, insulin-stimulated glucose uptake was abolished with siRNA-mediated GLUT3 knockdown. These results contradict previous reports that Clone 9 cells exclusively express GLUT1 and suggest GLUT3 is a key insulin-sensitive glucose transporter required for insulin-stimulated glucose uptake by Clone 9 cells.
Department of Human Nutritional Sciences, University of Manitoba, 209 Human Ecology Building, Winnipeg, Manitoba R3T 292, Canada; Canadian Centre for Agri-Food Research in Health and Medicine, St. Boniface Hospital Research Centre, 351 Taché Avenue, Winnipeg, Manitoba R2H 2A6, Canada; Department of Kinesiology and Applied Health, University of Winnipeg, 515 Portage Avenue, Winnipeg, Manitoba R3B 2E9, Canada. Electronic address: d.stringer@uwinnipeg.ca.
Danielle M
DM
Defries
Department of Human Nutritional Sciences, University of Manitoba, 209 Human Ecology Building, Winnipeg, Manitoba R3T 292, Canada; Department of Physiology and Pathophysiology, University of Manitoba, 432 Basic Medical Sciences Building, 745 Bannatyne Avenue, Winnipeg, Manitoba R3E 0J9, Canada; Canadian Centre for Agri-Food Research in Health and Medicine, St. Boniface Hospital Research Centre, 351 Taché Avenue, Winnipeg, Manitoba R2H 2A6, Canada. Electronic address: carla.taylor@umanitoba.ca.
Carla G
CG
Taylor
Department of Human Nutritional Sciences, University of Manitoba, 209 Human Ecology Building, Winnipeg, Manitoba R3T 292, Canada; Department of Physiology and Pathophysiology, University of Manitoba, 432 Basic Medical Sciences Building, 745 Bannatyne Avenue, Winnipeg, Manitoba R3E 0J9, Canada; Canadian Centre for Agri-Food Research in Health and Medicine, St. Boniface Hospital Research Centre, 351 Taché Avenue, Winnipeg, Manitoba R2H 2A6, Canada. Electronic address: pzahradka@sbrc.ca.
Peter
P
Zahradka
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002999:Clone Cells; D051274:Glucose Transporter Type 3 / Q000378:metabolism*; D007328:Insulin / Q000008:administration & dosage*; D008099:Liver / Q000378:metabolism*; D051379:Mice; D021381:Protein Transport
D051274:Glucose Transporter Type 3; D007328:Insulin; C495689:Slc2a3 protein, mouse
Clone 9 liver cells; GLUT1; GLUT3; Glucose transport; Insulin