Combined Transcriptomic and Proteomic Approach to Identify Toxicity Pathways in Early Life Stages of Japanese Medaka (Oryzias latipes) Exposed to 1,2,5,6-Tetrabromocyclooctane (TBCO).
Currently, the novel brominated flame retardant 1,2,5,6-tetrabromocyclooctane (TBCO) is considered a potential replacement for hexabromocyclododecane (HBCD). Therefore, use of TBCO could increase in the near future. To assess potential toxicological risks to aquatic organisms, embryos of Japanese medaka (Oryzias latipes) were exposed to 10, 100, or 1000 μg/L TBCO from 2 h postfertilization until 1 day post-hatch. TBCO accumulated in embryos in the order of 0.43-1.3 × 10(4)-fold, and the rate constant of accumulation was 1.7-1.8 per day. The number of days to hatch and the hatching success of embryos exposed to the medium and the greatest concentrations of TBCO were impaired. Responses of the transcriptome (RNA-seq) and proteome were characterized in embryos exposed to 100 μg/L TBCO because this was the least concentration of TBCO that caused an effect on hatching. Consistent with effects on hatching, proteins whose abundances were reduced by exposure to TBCO were enriched in embryo development and hatching pathways. Also, on the basis of the responses of transcriptome and proteome, it was predicted that TBCO might impair vision and contraction of cardiac muscle, respectively, and these effects were confirmed by targeted bioassays. This study provided a comprehensive understanding of effects of TBCO on medaka at early life stages and illustrated the power of "omics" to explain and predict phenotypic responses to chemicals.
Toxicology Centre, University of Saskatchewan , Saskatoon, Saskatchewan S7N 5B3, Canada.
Jianxian
J
Sun
School of Environment and Sustainability, University of Saskatchewan , Saskatoon, Saskatchewan S7N 5C8, Canada.
Song
S
Tang
Toxicology Centre, University of Saskatchewan , Saskatoon, Saskatchewan S7N 5B3, Canada.
Hui
H
Peng
Toxicology Centre, University of Saskatchewan , Saskatoon, Saskatchewan S7N 5B3, Canada.
David M V
DM
Saunders
Toxicology Centre, University of Saskatchewan , Saskatoon, Saskatchewan S7N 5B3, Canada.
Jon A
JA
Doering
Toxicology Centre, University of Saskatchewan , Saskatoon, Saskatchewan S7N 5B3, Canada.|School of Environment and Sustainability, University of Saskatchewan , Saskatoon, Saskatchewan S7N 5C8, Canada.
Markus
M
Hecker
Toxicology Centre, University of Saskatchewan , Saskatoon, Saskatchewan S7N 5B3, Canada.|School of Environment and Sustainability, University of Saskatchewan , Saskatoon, Saskatchewan S7N 5C8, Canada.
Paul D
PD
Jones
Toxicology Centre, University of Saskatchewan , Saskatoon, Saskatchewan S7N 5B3, Canada.|Department of Veterinary Biomedical Sciences, University of Saskatchewan , Saskatoon, Saskatchewan S7N 5B4, Canada.|Zoology Department, Center for Integrative Toxicology, Michigan State University , East Lansing, Michigan 48824, United States.|School of Biological Sciences, University of Hong Kong , Hong Kong Special Administrative Region 999077, People's Republic of China.|State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University , Nanjing 210093, People's Republic of China.
John P
JP
Giesy
Toxicology Centre, University of Saskatchewan , Saskatoon, Saskatchewan S7N 5B3, Canada.
Steve
S
Wiseman
D000818:Animals; D009990:Oryzias / Q000378:metabolism*; D040901:Proteomics; D014874:Water Pollutants, Chemical / Q000633:toxicity
D014874:Water Pollutants, Chemical