Isolation, identification, and optimal cultivation of a marine bacterium antagonistic to Magnaporthe grisea.
In this paper, a plate confrontation method was used to isolate bacteria antagonistic to the rice blast fungus Magnaporthe grisea from samples collected from China's Dalian Bay. The antagonist strain LM-031 was obtained. We studied this strain's morphological, physiological and biochemical characteristics and analyzed its 16S rDNA sequence. We compared the effects of different culture conditions (type of media, carbon and nitrogen source, incubation temperature and time, and initial pH value) on the inhibitory effect against M. grisea. Strain LM-031 was preliminarily identified as Bacillus pumilus and was found to strongly inhibit M. grisea, especially when grown on BPY medium at an initial pH 7 for 72 h at 30°C. The optimum carbon and nitrogen sources for growth were lactose and peptone, respectively. The most suitable carbon and nitrogen sources for production of active substances were glucose and NH4Cl, respectively. Our results show that development and utilization of B. pumilus LM-031 has great potential for biological control of M. grisea.
College of Environment and Resources, Dalian Nationalities University, Dalian, Liaoning, China.
W J
WJ
Zhang
College of Environment and Resources, Dalian Nationalities University, Dalian, Liaoning, China.
P
P
Guo
College of Environment and Resources, Dalian Nationalities University, Dalian, Liaoning, China.|College of Life Sciences, Liaoning Normal University, Dalian, Liaoning, China.
M
M
Liu
College of Environment and Resources, Dalian Nationalities University, Dalian, Liaoning, China.
B L
BL
Yang
College of Life Sciences, Liaoning Normal University, Dalian, Liaoning, China.
J H
JH
Wang
College of Environment and Resources, Dalian Nationalities University, Dalian, Liaoning, China.
J
J
Jiang
D000069979:Bacillus pumilus / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism / Q000502:physiology*; D002681:China; D004275:DNA, Ribosomal / Q000235:genetics; D020082:Magnaporthe / Q000502:physiology*; D012275:Oryza; D010935:Plant Diseases / Q000382:microbiology* / Q000628:therapy*; D012623:Seawater / Q000382:microbiology
D004275:DNA, Ribosomal