Design and Implementation of High-Throughput Screening Assays.
HTS remains at the core of the drug discovery process, and so it is critical to design and implement HTS assays in a comprehensive fashion involving scientists from the disciplines of biology, chemistry, engineering, and informatics. This requires careful consideration of many options and variables, starting with the choice of screening strategy and ending with the discovery of lead compounds. At every step in this process, there are decisions to be made that can greatly impact the outcome of the HTS effort, to the point of making it a success or a failure. Although specific guidelines should be established to ensure that the screening assay reaches an acceptable level of quality, many choices require pragmatism and the ability to compromise opposing forces.
Alternative Drug Discovery, GSK Pharmaceuticals, Gunnels Wood Road, Stevenage, Herts, SG1 2NY, UK. David.J.Powell@gsk.com.
David J
DJ
Powell
GSK Pharmaceuticals R&D, Philadelphia, PA, USA.
Robert P
RP
Hertzberg
Alternative Drug Discovery, GSK Pharmaceuticals, 1250 South Collegeville Road, Upper Providence, PA, 19426, USA.
Ricardo
R
Macarrόn
D000818:Animals; D001671:Biochemistry / Q000379:methods; D003584:Cytological Techniques / Q000379:methods; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D057075:Enzyme Assays / Q000379:methods; D057166:High-Throughput Screening Assays / Q000379:methods*; D006801:Humans; D059629:Signal-To-Noise Ratio; D054928:Validation Studies as Topic
Bioassay; Drug screening; Human; Methodology; Phenotypic