[Diallyl disulfide inhibits invasion and metastasis of MCF-7 breast cancer cells in vitro by down-regulating p38 activity].
OBJECTIVE
To investigate the effect of diallyl disulfide (DADS) on invasion and metastasis of human breast cancer MCF-7 cells and explore the possible mechanism.


METHODS
MCF-7 cells treated with 100, 200, and 400 µmol/L of DADS for 24 h were examined for cell invasion and migration capacities using Transwell assay and wound healing assay, respectively. The protein expression of E-cadherin, vimentin, MMP-9 and p-p38 in the cells were detected with Western blotting. The effect of transforming growth factor-β1 (TGF-β1) as the agonist of p38 activity was tested in antagonizing the effects of DADS.


RESULTS
DADS inhibited the invasion and migration of MCF-7 cells in a dose-dependent manner, down-regulated the protein expression of Vimentin and MMP-9 and up-regulated E-cadherin expression in the cells. Treatment with TGF-β1 to up-regulate p38 activity obviously antagonized the inhibitory effect of DADS on the invasion and metastasis of MCF-7 cells.


CONCLUSIONS
DADS can inhibit the invasion and metastasis of MCF-7 cells in vitro by down-regulating p38 activity.
Institute of Pharmacy and Pharmacology, University of South China, Hengyang 421001, China. E-mail: 1205994004@qq.com.
Xiao-Xiao
XX
Chen
Xiao-Wang
XW
Liu
Zhi-Gang
ZG
Zhou
Xue-Yan
XY
Chen
Li-Ding
LD
Li
Ting
T
Xiong
Lu
L
Peng
Jian
J
Tu
D000498:Allyl Compounds / Q000494:pharmacology*; D015703:Antigens, CD; D001943:Breast Neoplasms / Q000473:pathology*; D015820:Cadherins / Q000378:metabolism; D004220:Disulfides / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D061986:MCF-7 Cells / Q000187:drug effects; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D048290:Mitogen-Activated Protein Kinase 11 / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology; D014746:Vimentin / Q000378:metabolism
D000498:Allyl Compounds; D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; D004220:Disulfides; D053773:Transforming Growth Factor beta1; D014746:Vimentin; C028009:diallyl disulfide; D048290:Mitogen-Activated Protein Kinase 11; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9