Serum Biochemistry of Lumpy Skin Disease Virus-Infected Cattle.
Lumpy skin disease is an economically important poxvirus disease of cattle. Vaccination is the main method of control but sporadic outbreaks have been reported in Turkey. This study was carried out to determine the changes in serum biochemical values of cattle naturally infected with lumpy skin disease virus (LSDV). For this study, blood samples in EDTA, serum samples, and nodular skin lesions were obtained from clinically infected animals (n = 15) whereas blood samples in EDTA and serum samples were collected from healthy animals (n = 15). A quantitative real-time PCR method was used to detect Capripoxvirus (CaPV) DNA in clinical samples. A real-time PCR high-resolution melt assay was performed to genotype CaPVs. Serum cardiac, hepatic, and renal damage markers and lipid metabolism products were measured by autoanalyzer. LSDV nucleic acid was detected in all samples which were obtained from clinically infected cattle. The results of serum biochemical analysis showed that aspartate aminotransferase, alkaline phosphatase, total protein, and creatinine concentrations were markedly increased in serum from infected animals. However, there were no significant differences in the other biochemical parameters evaluated. The results of the current study suggest that liver and kidney failures occur during LSDV infection. These findings may help in developing effective treatment strategies in LSDV infection.
Department of Molecular Microbiology, Veterinary Control Institute, Meram, 42080 Konya, Turkey.
Murat
0000-0002-9604-3341
M
Şevik
Department of Virology, Faculty of Veterinary Medicine, Selçuk University, 42000 Konya, Turkey.
Oğuzhan
O
Avci
Department of Molecular Microbiology, Veterinary Control Institute, Meram, 42080 Konya, Turkey.
Müge
M
Doğan
Agriculture and Rural Development Support Institution, 03000 Afyonkarahisar, Turkey.
Ömer Barış
ÖB
İnce
D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D018151:Capripoxvirus / Q000235:genetics / Q000472:pathogenicity; D002417:Cattle; D003404:Creatinine / Q000097:blood; D004279:DNA, Viral; D017093:Liver Failure / Q000097:blood / Q000378:metabolism / Q000821:virology; D008166:Lumpy Skin Disease / Q000097:blood* / Q000378:metabolism / Q000821:virology*; D008167:Lumpy skin disease virus / Q000235:genetics / Q000472:pathogenicity*; D011506:Proteins / Q000378:metabolism; D051437:Renal Insufficiency / Q000097:blood / Q000378:metabolism / Q000821:virology
D004279:DNA, Viral; D011506:Proteins; D003404:Creatinine; D001219:Aspartate Aminotransferases; D000469:Alkaline Phosphatase