The distribution of α-kleisin during meiosis in the holocentromeric plant Luzula elegans.
Holocentric chromosomes occur in a number of independent eukaryotic lineages, and they form holokinetic kinetochores along the entire poleward chromatid surfaces. Due to this alternative chromosome structure, Luzula elegans sister chromatids segregate already in anaphase I followed by the segregation of the homologues in anaphase II. However, not yet known is the localization and dynamics of cohesin and the structure of the synaptonemal complex (SC) during meiosis. We show here that the α-kleisin subunit of cohesin localizes at the centromeres of both mitotic and meiotic metaphase chromosomes and that it, thus, may contribute to assemble the centromere in L. elegans. This localization and the formation of a tripartite SC structure indicate that the prophase I behaviour of L. elegans is similar as in monocentric species.
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, 06466, Stadt Seeland, Germany.
Wei
W
Ma
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, 06466, Stadt Seeland, Germany.
Veit
V
Schubert
Institute of Bioinformatics and Systems Biology/Munich Information Center for Protein Sequences, Helmholtz Center Munich, German Research Center for Environmental Health, 85764, Neuherberg, Germany.|Division of Cell Biology, Department of Clinical and Experimental Medicine, Bioinformatics Infrastructure for Life Sciences, Linköping University, 558185, Linköping, Sweden.
Mihaela Maria
MM
Martis
Biocenter, Microscopy Unit, Martin Luther University Halle-Wittenberg, Weinbergweg 22, 06120, Halle, Germany.
Gerd
G
Hause
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, 06466, Stadt Seeland, Germany.
Zhaojun
Z
Liu
State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China.
Yi
Y
Shen
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, 06466, Stadt Seeland, Germany.
Udo
U
Conrad
State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China.
Wenqing
W
Shi
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, 06466, Stadt Seeland, Germany.
Uwe
U
Scholz
Leibniz Institute on Aging-Fritz-Lipmann-Institut e.V. (FLI), Beutenbergstraße 11, 07745, Jena, Germany.
Stefan
S
Taudien
State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China.
Zhukuan
Z
Cheng
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, 06466, Stadt Seeland, Germany. houben@ipk-gatersleben.de.
Andreas
A
Houben
D001324:Autoantigens / Q000235:genetics; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002503:Centromere / Q000378:metabolism*; D000076247:Centromere Protein A; D002842:Chromatids / Q000235:genetics; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism*; D020090:Chromosome Segregation / Q000235:genetics; D002875:Chromosomes / Q000235:genetics; D018386:Kinetochores / Q000378:metabolism; D019684:Magnoliopsida / Q000235:genetics* / Q000378:metabolism; D008540:Meiosis / Q000235:genetics*; D049469:Meiotic Prophase I / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D013573:Synaptonemal Complex / Q000648:ultrastructure*; D000097722:Cohesins
D001324:Autoantigens; D018797:Cell Cycle Proteins; D000076247:Centromere Protein A; D002868:Chromosomal Proteins, Non-Histone; D010940:Plant Proteins; D000097722:Cohesins
CENH3; Holocentric chromosome; Luzula elegans; Synaptonemal complex; α-kleisin