Anti-bacterial and anti-biofilm activity of probiotic bacteria against oral pathogens.
In this study, three lactic acid bacteria (LAB), isolated from barley, traditional dried meat and fermented olive were characterized and tested for their anti-bacterial and anti-biofilm activities against oral bacteria. Our results revealed that the tested LAB were γ-hemolytic and were susceptible to four antibiotics. All the strains were resistant to low pH, bile salt, pepsin and pancreatin. Furthermore, FB2 displayed a high aut-oaggregative phenotype (99.54%) while FF2 exhibited the best co-aggregation rate. Concerning the microbial adhesion to solvent, FB2 was the most hydrophobic strain (data obtained with chloroform and n-hexadecane). In addition Pediococcus pentosaceus FB2 and Lactobacillus brevis FF2 displayed a significant inhibitory effect against Streptococcus salivarius B468 (MIC = 10%). Moreover the selected strains were able to inhibit biofilm formation of Bacillus cereus ATCC14579 (MBIC50 = 28.16%) and S. salivarius B468 (MBIC50 = 42.28%). The selected LAB could be considered as candidate probiotics for further application in functional food and mainly in the prevention of oral diseases.
Laboratory of Analysis, Treatment and Valorization of Environmental Polluants and Products, Faculty of Pharmacy, Monastir University, Tunisia.
Fadia
F
Ben Taheur
College of Applied Medical Sciences, Medical Laboratory Department, Yanbu el Bahr, Taibah University, Al Madinah Al Monawarah, Saudi Arabia.
Bochra
B
Kouidhi
Laboratory of Analysis, Treatment and Valorization of Environmental Polluants and Products, Faculty of Pharmacy, Monastir University, Tunisia.
Kais
K
Fdhila
Laboratory of Analysis, Treatment and Valorization of Environmental Polluants and Products, Faculty of Pharmacy, Monastir University, Tunisia.
Hamouda
H
Elabed
Laboratory of Analysis, Treatment and Valorization of Environmental Polluants and Products, Faculty of Pharmacy, Monastir University, Tunisia.
Rihab
R
Ben Slama
Laboratory of Analysis, Treatment and Valorization of Environmental Polluants and Products, Faculty of Pharmacy, Monastir University, Tunisia.
Kacem
K
Mahdouani
Laboratory of Analysis, Treatment and Valorization of Environmental Polluants and Products, Faculty of Pharmacy, Monastir University, Tunisia.
Amina
A
Bakhrouf
College of Sciences, Yanbu el Bahr, Taibah University, Al Madinah Al Monawarah, Saudi Arabia. Electronic address: chaieb_mo@yahoo.fr.
Kamel
K
Chaieb
D000898:Antibiosis; D001409:Bacillus cereus / Q000254:growth & development* / Q000502:physiology; D018441:Biofilms / Q000254:growth & development*; D005516:Food Microbiology; D001467:Hordeum / Q000382:microbiology; D056546:Lactobacillales / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology*; D008826:Microbial Sensitivity Tests; D009055:Mouth / Q000382:microbiology*; D019936:Probiotics; D000070001:Streptococcus salivarius / Q000254:growth & development* / Q000502:physiology
Anti-bacterial; Anti-biofilm; Lactic acid bacteria; Oral strains; Probiotic