Single ingestion of soy β-conglycinin induces increased postprandial circulating FGF21 levels exerting beneficial health effects.
Soy protein β-conglycinin has serum lipid-lowering and anti-obesity effects. We showed that single ingestion of β-conglycinin after fasting alters gene expression in mouse liver. A sharp increase in fibroblast growth factor 21 (FGF21) gene expression, which is depressed by normal feeding, resulted in increased postprandial circulating FGF21 levels along with a significant decrease in adipose tissue weights. Most increases in gene expressions, including FGF21, were targets for the activating transcription factor 4 (ATF4), but not for peroxisome proliferator-activated receptor α. Overexpression of a dominant-negative form of ATF4 significantly reduced β-conglycinin-induced increases in hepatic FGF21 gene expression. In FGF21-deficient mice, β-conglycinin effects were partially abolished. Methionine supplementation to the diet or primary hepatocyte culture medium demonstrated its importance for activating liver or hepatocyte ATF4-FGF21 signaling. Thus, dietary β-conglycinin intake can impact hepatic and systemic metabolism by increasing the postprandial circulating FGF21 levels.
Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.|Institute of Gerontology, The University of Tokyo, Tokyo 113-8656, Japan.
Tsutomu
T
Hashidume
Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.
Asuka
A
Kato
Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.
Tomohiro
T
Tanaka
Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.
Shoko
S
Miyoshi
Medical Innovation Center, Kyoto University Graduate School of Medicine, Sakyo, Kyoto 606-8507, Japan.
Nobuyuki
N
Itoh
Department of Food Science and Nutrition, Nara Women's University, Kita-Uoya-Nishi-Machi, Nara, 630-8506, Japan.
Rieko
R
Nakata
Department of Food Science and Nutrition, Nara Women's University, Kita-Uoya-Nishi-Machi, Nara, 630-8506, Japan.
Hiroyasu
H
Inoue
RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa, Japan; Faculty of Agriculture, Yamagata University, Tsuruoka-shi, Yamagata 997-8555, Japan.
Akira
A
Oikawa
Institute for Food Science, Hirosaki University, Aomori 038-0012, Japan.
Yuji
Y
Nakai
Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.
Makoto
M
Shimizu
Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.
Jun
J
Inoue
Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan.
Ryuichiro
R
Sato
D051701:Activating Transcription Factor 4 / Q000378:metabolism; D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D052179:Antigens, Plant / Q000494:pharmacology*; D002478:Cells, Cultured; D004032:Diet; D004044:Dietary Proteins / Q000097:blood*; D004734:Energy Metabolism; D005346:Fibroblast Growth Factors / Q000096:biosynthesis* / Q000097:blood* / Q000235:genetics; D005916:Globulins / Q000494:pharmacology*; D022781:Hepatocytes / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D047493:PPAR alpha / Q000235:genetics / Q000378:metabolism; D019518:Postprandial Period / Q000502:physiology; D055314:Seed Storage Proteins / Q000494:pharmacology*; D030262:Soybean Proteins / Q000494:pharmacology*
D052179:Antigens, Plant; C081452:Atf4 protein, mouse; D004044:Dietary Proteins; D005916:Globulins; D047493:PPAR alpha; D055314:Seed Storage Proteins; D030262:Soybean Proteins; C013894:beta-conglycinin protein, Glycine max; C414620:fibroblast growth factor 21; D051701:Activating Transcription Factor 4; D005346:Fibroblast Growth Factors