Frutalin reduces acute and neuropathic nociceptive behaviours in rodent models of orofacial pain.
Orofacial pain is a highly prevalent clinical condition, yet difficult to control effectively with available drugs. Much attention is currently focused on the anti-inflammatory and antinociceptive properties of lectins. The purpose of this study was to evaluate the antinociceptive effect of frutalin (FTL) using rodent models of inflammatory and neuropathic orofacial pain. Acute pain was induced by formalin, glutamate or capsaicin (orofacial model) and hypertonic saline (corneal model). In one experiment, animals were pretreated with l-NAME and naloxone to investigate the mechanism of antinociception. The involvement of the lectin domain in the antinociceptive effect of FTL was verified by allowing the lectin to bind to its specific ligand. In another experiment, animals pretreated with FTL or saline were submitted to the temporomandibular joint formalin test. In yet another, animals were submitted to infraorbital nerve transection to induce chronic pain, followed by induction of thermal hypersensitivity using acetone. Motor activity was evaluated with the rotarod test. A molecular docking was performed using the TRPV1 channel. Pretreatment with FTL significantly reduced nociceptive behaviour associated with acute and neuropathic pain, especially at 0.5 mg/kg. Antinociception was effectively inhibited by l-NAME and d-galactose. In line with in vivo experiments, docking studies indicated that FTL may interact with TRPV1. Our results confirm the potential pharmacological relevance of FTL as an inhibitor of orofacial nociception in acute and chronic pain mediated by TRPA1, TRPV1 and TRPM8 receptor.
Experimental Biology Centre (NUBEX), University of Fortaleza (UNIFOR), Fortaleza, Ceará, Brazil.
Marina B M V
MB
Damasceno
Experimental Biology Centre (NUBEX), University of Fortaleza (UNIFOR), Fortaleza, Ceará, Brazil.
José de Maria A
Jde M
de Melo Júnior
Experimental Biology Centre (NUBEX), University of Fortaleza (UNIFOR), Fortaleza, Ceará, Brazil.
Sacha Aubrey A R
SA
Santos
Department of Physical Therapy, University of Toronto (UofT), Toronto, Ontario, Canada.
Luana T M
LT
Melo
Experimental Biology Centre (NUBEX), University of Fortaleza (UNIFOR), Fortaleza, Ceará, Brazil.
Laura Hévila I
LH
Leite
Department of Biochemistry and Molecular Biology, Federal University of Ceará (UFC), Fortaleza, Ceará, Brazil.
Antonio E
AE
Vieira-Neto
Experimental Biology Centre (NUBEX), University of Fortaleza (UNIFOR), Fortaleza, Ceará, Brazil.
Renato de A
Rde A
Moreira
Experimental Biology Centre (NUBEX), University of Fortaleza (UNIFOR), Fortaleza, Ceará, Brazil.
Ana Cristina de O
AC
Monteiro-Moreira
Experimental Biology Centre (NUBEX), University of Fortaleza (UNIFOR), Fortaleza, Ceará, Brazil. Electronic address: adrirolim@unifor.br.
Adriana R
AR
Campos
D059787:Acute Pain / Q000188:drug therapy / Q000378:metabolism; D000700:Analgesics / Q000302:isolation & purification / Q000627:therapeutic use*; D000818:Animals; D031622:Artocarpus / Q000737:chemistry; D004195:Disease Models, Animal; D005157:Facial Pain / Q000188:drug therapy* / Q000378:metabolism; D037161:Galectins / Q000302:isolation & purification / Q000627:therapeutic use*; D051379:Mice; D062105:Molecular Docking Simulation; D009437:Neuralgia; D017208:Rats, Wistar; D050053:TRPM Cation Channels / Q000378:metabolism; D050916:TRPV Cation Channels / Q000378:metabolism; D050051:Transient Receptor Potential Channels / Q000378:metabolism
D000700:Analgesics; D037161:Galectins; D050053:TRPM Cation Channels; D050916:TRPV Cation Channels; D050051:Transient Receptor Potential Channels; C570811:frutalin
Artocarpus incisa L; Frutalin; Orofacial nociception