Surfactant-activated lipase hybrid nanoflowers with enhanced enzymatic performance.
Increasing numbers of materials have been extensively used as platforms for enzyme immobilization to improve catalytic performance. However, activity of the most of the enzymes was declined after immobilization. Here, we develop a surfactant-activated lipase-inorganic flowerlike hybrid nanomaterials with rational design based on interfacial activation and self-assembly. The resulting surfactant-activated lipase-inorganic hybird nanoflower (activated hNF-lipase) exhibited 460% and 200% higher activity than native lipase and conventional lipase-inorganic hybird nanoflower (hNF-lipase). Furthermore, the activated hNF-lipase displayed good reusability due to its monodispersity and mechanical properties, and had excellent long-time stability. The superior catalytic performances were attributed to both the conformational modulation of surfactants and hierarchical structure of nanoflowers, which not only anchored lipases in an active form, but also decreased the enzyme-support negative interaction and mass-transfer limitations. This new biocatalytic system is promising to find widespread use in applications related to biomedicine, biosensor, and biodiesel.
Research Center for Fermentation Engineering of Hebei, College of Bioscience and Bioengineering, Hebei University of Science and Technology, 26 Yuxiang Street, Shijiazhang 050000, P R China.|Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, No 29, 13th, Avenue, Tianjin Economic and Technological Development Area (TEDA), Tianjin 300457, P R China.
Jiandong
J
Cui
Research Center for Fermentation Engineering of Hebei, College of Bioscience and Bioengineering, Hebei University of Science and Technology, 26 Yuxiang Street, Shijiazhang 050000, P R China.
Yamin
Y
Zhao
Research Center for Fermentation Engineering of Hebei, College of Bioscience and Bioengineering, Hebei University of Science and Technology, 26 Yuxiang Street, Shijiazhang 050000, P R China.
Ronglin
R
Liu
Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, No 29, 13th, Avenue, Tianjin Economic and Technological Development Area (TEDA), Tianjin 300457, P R China.
Cheng
C
Zhong
Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, No 29, 13th, Avenue, Tianjin Economic and Technological Development Area (TEDA), Tianjin 300457, P R China.
Shiru
S
Jia
D000494:Allosteric Regulation; D055162:Biocatalysis; D056804:Biofuels; D015374:Biosensing Techniques; D004345:Drug Industry; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008049:Lipase / Q000737:chemistry / Q000378:metabolism*; D049329:Nanostructures / Q000706:statistics & numerical data*; D011487:Protein Conformation; D013501:Surface-Active Agents / Q000737:chemistry*
D056804:Biofuels; D004800:Enzymes, Immobilized; D013501:Surface-Active Agents; D008049:Lipase