Exercise in an electrotactic flow chamber ameliorates age-related degeneration in Caenorhabditis elegans.
Degeneration is a senescence process that occurs in all living organisms. Although tremendous efforts have been exerted to alleviate this degenerative tendency, minimal progress has been achieved to date. The nematode, Caenorhabditis elegans (C. elegans), which shares over 60% genetic similarities with humans, is a model animal that is commonly used in studies on genetics, neuroscience, and molecular gerontology. However, studying the effect of exercise on C. elegans is difficult because of its small size unlike larger animals. To this end, we fabricated a flow chamber, called "worm treadmill," to drive worms to exercise through swimming. In the device, the worms were oriented by electrotaxis on demand. After the exercise treatment, the lifespan, lipofuscin, reproductive capacity, and locomotive power of the worms were analyzed. The wild-type and the Alzheimer's disease model strains were utilized in the assessment. Although degeneration remained irreversible, both exercise-treated strains indicated an improved tendency compared with their control counterparts. Furthermore, low oxidative stress and lipofuscin accumulation were also observed among the exercise-treated worms. We conjecture that escalated antioxidant enzymes imparted the worms with an extra capacity to scavenge excessive oxidative stress from their bodies, which alleviated the adverse effects of degeneration. Our study highlights the significance of exercise in degeneration from the perspective of the simple life form, C. elegans.
Department of Biomedical Engineering, National Cheng Kung University, Taiwan.|Medical Device Innovation Center, National Cheng Kung University, Taiwan.
Han-Sheng
HS
Chuang
Department of Biomedical Engineering, National Cheng Kung University, Taiwan.
Wan-Jung
WJ
Kuo
Department of Biomedical Engineering, National Cheng Kung University, Taiwan.
Chia-Lin
CL
Lee
Department of Sports Medicine, Kaohsiung Medical University, Taiwan.
I-Hua
IH
Chu
Department of Biochemical and Molecular Biology, National Cheng Kung University, Taiwan.
Chang-Shi
CS
Chen
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017173:Caenorhabditis elegans / Q000254:growth & development* / Q000378:metabolism; D004558:Electric Stimulation / Q000295:instrumentation*; D008136:Longevity; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D018384:Oxidative Stress / Q000187:drug effects; D010805:Physical Conditioning, Animal
D000975:Antioxidants