A Simplified Direct Lipid Mixing Lipoplex Preparation: Comparison of Liposomal-, Dimethylsulfoxide-, and Ethanol-Based Methods.
Established transfection methodology often uses commercial reagents, which must be formed into liposomes in a sequence of about half a dozen steps. The simplified method reported here is a direct lipid mixing approach that requires fewer steps, less manipulation, and is less time-consuming. Results are comparable to those obtained with more commonly used methods, as judged by a variety of analytical techniques and by comparisons of transfection results. The method reported here may be applied to non-liposome-forming compounds, thereby greatly expanding the range of structures that can be tested for transfection ability.
Center for Nanoscience, University of Missouri - St. Louis, 1 University Blvd., St. Louis, MO 63121, USA.|Department of Chemistry &Biochemistry, University of Missouri - St. Louis, 1 University Blvd., St. Louis, MO 63121, USA.
Joseph W
JW
Meisel
Center for Nanoscience, University of Missouri - St. Louis, 1 University Blvd., St. Louis, MO 63121, USA.|Department of Chemistry &Biochemistry, University of Missouri - St. Louis, 1 University Blvd., St. Louis, MO 63121, USA.|Department of Biology, University of Missouri - St. Louis, 1 University Blvd., St. Louis, MO 63121, USA.
George W
GW
Gokel
D004247:DNA / Q000008:administration & dosage / Q000737:chemistry* / Q000235:genetics; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D000431:Ethanol / Q000737:chemistry; D018014:Gene Transfer Techniques; D008055:Lipids / Q000737:chemistry* / Q000235:genetics / Q000494:pharmacology; D008081:Liposomes / Q000737:chemistry* / Q000494:pharmacology; D014162:Transfection / Q000379:methods*
D008055:Lipids; D008081:Liposomes; D000431:Ethanol; D004247:DNA; D004121:Dimethyl Sulfoxide