Association between quantitative measures obtained using fluorescence-based methods and activity status of occlusal caries lesions in primary molars.
BACKGROUND
Fluorescence-based methods (FBM) can add objectiveness to diagnosis strategy for caries. Few studies, however, have focused on the evaluation of caries activity.


OBJECTIVE
To evaluate the association between quantitative measures obtained with FBM, clinical parameters acquired from the patients, caries detection, and assessment of activity status in occlusal surfaces of primary molars.


METHODS
Six hundred and six teeth from 113 children (4-14 years) were evaluated. The presence of a biofilm, caries experience, and the number of active lesions were recorded. The teeth were assessed using FBM: DIAGNOdent pen (Lfpen) and Quantitative light-induced fluorescence (QLF). As reference standard, all teeth were evaluated using the ICDAS (International Caries Detection and Assessment System) associated with clinical activity assessments. Multilevel regressions compared the FBM values and evaluated the association between the FBM measures and clinical variables related to the caries activity.


RESULTS
The measures from the FBM were higher in cavitated lesions. Only, ∆F values distinguished active and inactive lesions. The LFpen measures were higher in active lesions, at the cavitated threshold (56.95 ± 29.60). Following regression analyses, only the presence of visible biofilm on occlusal surfaces (adjusted prevalence ratio = 1.43) and ∆R values of the teeth (adjusted prevalence ratio = 1.02) were associated with caries activity.


CONCLUSIONS
Some quantitative measures from FBM parameters are associated with caries activity evaluation, which is similar to the clinical evaluation of the presence of visible biofilm.
Department of Pediatric Dentistry, School of Dentistry, Universidade Cruzeiro do Sul, São Paulo, Brazil.|Department of Pediatric Dentistry, School of Dentistry, Universidade de São Paulo, São Paulo, Brazil.
Tatiane Fernandes
TF
Novaes
Department of Pediatric Dentistry, School of Dentistry, Universidade de São Paulo, São Paulo, Brazil.
Alessandra
A
Reyes
Department of Pediatric Dentistry, School of Dentistry, Universidade Guarulhos, Guarulhos, Brazil.
Ronilza
R
Matos
Department of Pediatric Dentistry, School of Dentistry, Universidade de São Paulo, São Paulo, Brazil.
Laura Regina
LR
Antunes-Pontes
Department of Pediatric Dentistry, School of Dentistry, Universidade Cruzeiro do Sul, São Paulo, Brazil.
Renata Pereira de Samuel
RP
Marques
Department of Pediatric Dentistry, School of Dentistry, Universidade de São Paulo, São Paulo, Brazil.
Mariana Minatel
MM
Braga
Department of Pediatric Dentistry, School of Dentistry, Universidade Cruzeiro do Sul, São Paulo, Brazil.
Michele Baffi
MB
Diniz
Department of Pediatric Dentistry, School of Dentistry, Universidade de São Paulo, São Paulo, Brazil.
Fausto Medeiros
FM
Mendes
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D003731:Dental Caries / Q000000981:diagnostic imaging*; D003732:Dental Caries Activity Tests / Q000295:instrumentation* / Q000379:methods*; D003766:Dental Occlusion; D005260:Female; D006801:Humans; D008297:Male; D008963:Molar; D061848:Optical Imaging / Q000295:instrumentation* / Q000379:methods*; D011861:Radiography, Dental / Q000295:instrumentation* / Q000379:methods*; D012680:Sensitivity and Specificity; D013223:Statistics as Topic; D014094:Tooth, Deciduous