Plant-specific DUF1110 protein from Oryza sativa: expression, purification and crystallization.
The Os01T0156300 protein from Oryza sativa has been classified into the domain of unknown function (DUF) family DUF1110. DUF1110 family members exist in monocotyledons but not in dicotyledons, and share no sequence identity with proteins for which structures have been reported. In this study, the Os01T0156300 protein was crystallized using the hanging-drop vapour-diffusion method. X-ray diffraction data were collected to 1.84 Å resolution. The crystal belonged to space group P21, with unit-cell parameters a = 89.9, b = 89.8, c = 107.1 Å, β = 106.6°. The asymmetric unit was estimated to contain 6-11 molecules.
Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita-shi, Osaka 565-0871, Japan.
Kenichi
K
Harada
Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita-shi, Osaka 565-0871, Japan.
Eiki
E
Yamashita
Department of Advanced Bioscience, Kindai University, 3327-204 Nakamachi, Nara 631-8505, Japan.
Kento
K
Inoue
Department of Advanced Bioscience, Kindai University, 3327-204 Nakamachi, Nara 631-8505, Japan.
Koji
K
Yamaguchi
Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita-shi, Osaka 565-0871, Japan.
Toshimichi
T
Fujiwara
Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita-shi, Osaka 565-0871, Japan.
Atsushi
A
Nakagawa
Department of Advanced Bioscience, Kindai University, 3327-204 Nakamachi, Nara 631-8505, Japan.
Tsutomu
T
Kawasaki
Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita-shi, Osaka 565-0871, Japan.
Chojiro
C
Kojima
D000595:Amino Acid Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D012275:Oryza / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*
D010940:Plant Proteins
DUF1110; Oryza sativa; Os01T0156300; PAMP-triggered immunity; interactor for OsPUB44; plant-specific protein