Use of Coronary Ultrasound Imaging to Evaluate Ventricular Function in Adult Zebrafish.
So far, imaging of the adult zebrafish heart and assessment of heart failure in adult zebrafish have been very limited. Here, we describe a new method for in vivo imaging of the hypertrabeculated heart of the adult zebrafish using miniaturized cardiac ultrasound catheters obtained from the cardiac catheterization laboratory. This method allows the observation of the ventricle of zebrafish and the assessment of ventricular diameters during diastole and systole, as well as heart rate and fractional shortening. Significant changes in these parameters were detected through the use of an adult zebrafish heart failure model induced by chronic anemia. This imaging technique opens the door to detailed in vivo analysis of the adult heart failure phenotype in zebrafish.
1 Cardiovascular Research Unit, Luxembourg Institute of Health , Strassen, Luxembourg .
Isabelle
I
Ernens
1 Cardiovascular Research Unit, Luxembourg Institute of Health , Strassen, Luxembourg .
Andrew I
AI
Lumley
1 Cardiovascular Research Unit, Luxembourg Institute of Health , Strassen, Luxembourg .
Yvan
Y
Devaux
1 Cardiovascular Research Unit, Luxembourg Institute of Health , Strassen, Luxembourg .|2 Division of Cardiology, Centre Hospitalier de Luxembourg , Luxembourg, Luxembourg .
Daniel R
DR
Wagner
D000818:Animals; D009202:Cardiomyopathies / Q000000981:diagnostic imaging* / Q000503:physiopathology; D004195:Disease Models, Animal; D006352:Heart Ventricles / Q000000981:diagnostic imaging*; D008297:Male; D014463:Ultrasonography; D016276:Ventricular Function; D015027:Zebrafish / Q000502:physiology*
adult zebrafish; cardiac function; heart failure; miniaturized ultrasound system