title: Vaginal delivery of carboplatin-loaded thermosensitive hydrogel to prevent local cervical cancer recurrence in mice.
abstract: Local tumor recurrence after cervical cancer surgery remains a clinical problem. Vaginal delivery of thermosensitive hydrogel may be suited to reduce tumor relapse rate with more efficacy and safety. A pilot study was carried out to evaluate the efficacy of carboplatin-loaded poloxamer hydrogel to prevent local recurrence of cervical cancer after surgery. In vivo vaginal retention evaluation of 27% poloxamer hydrogel in mice was proven to be a suitable vaginal drug delivery formulation due to its low gelation temperature. A mimic orthotopic cervical/vaginal cancer recurrence model after surgery was established by injecting murine cervical cancer cell line U14 into the vaginal submucosa to simulate the residual tumor cells infiltrated in the surgical site, followed by drug administration 24 h later to interfere with the formation/recurrence of the tumor. By infusing fluorescein sodium-loaded hydrogel into the vagina of mice, a maximized accumulation of fluorescein sodium (Flu) in the vagina was achieved and few signals were observed in other organs. When used in the prevention of the cervical cancer formation/recurrence in mice, the carboplatin-loaded poloxamer hydrogel exhibited great efficacy and systemic safety. In conclusion, thermosensitive hydrogel presents a simple, practical approach for the local drug delivery via vagina against cervical cancer recurrence.
authors.affiliation: a China-Japan Union Hospital of Jilin University , Changchun , People's Republic of China.
authors.forename: Xue
authors.initials: X
authors.lastname: Wang
authors.affiliation: b The First Hospital of Jilin University , Changchun , People's Republic of China , and.
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.affiliation: c Xuzhou Central Hospital Affiliated to Southeast University , Xuzhou , People's Republic of China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Wu
authors.affiliation: a China-Japan Union Hospital of Jilin University , Changchun , People's Republic of China.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Li
mesh_terms: D000818:Animals; D016190:Carboplatin / Q000008:administration & dosage*; D045744:Cell Line, Tumor; D002626:Chemistry, Pharmaceutical / Q000379:methods; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods; D005260:Female; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000008:administration & dosage*; D051379:Mice; D009364:Neoplasm Recurrence, Local / Q000517:prevention & control*; D010865:Pilot Projects; D020442:Poloxamer / Q000737:chemistry; D013696:Temperature; D002583:Uterine Cervical Neoplasms / Q000517:prevention & control*; D014621:Vagina / Q000378:metabolism*
chemical_list: D004337:Drug Carriers; D020442:Poloxamer; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D016190:Carboplatin
keywords: Vaginal delivery; local cervical cancer recurrence; local chemotherapy; poloxamer; thermosensitive hydrogel