title: A systematic review and meta-analysis to determine the effect of sperm DNA damage onin vitrofertilization and intracytoplasmic sperm injection outcome.
abstract: Sperm DNA damage is prevalent among infertile men and is known to influence natural reproduction. However, the impact of sperm DNA damage on assisted reproduction outcomes remains controversial. Here, we conducted a meta-analysis of studies on sperm DNA damage (assessed by SCSA, TUNEL, SCD, or Comet assay) and clinical pregnancy after IVF and/or ICSI treatment from MEDLINE, EMBASE, and PUBMED database searches for this analysis. We identified 41 articles (with a total of 56 studies) including 16 IVF studies, 24 ICSI studies, and 16 mixed (IVF + ICSI) studies. These studies measured DNA damage (by one of four assays: 23 SCSA, 18 TUNEL, 8 SCD, and 7 Comet) and included a total of 8068 treatment cycles (3734 IVF, 2282 ICSI, and 2052 mixed IVF + ICSI). The combined OR of 1.68 (95% CI: 1.49-1.89; P < 0.0001) indicates that sperm DNA damage affects clinical pregnancy following IVF and/or ICSI treatment. In addition, the combined OR estimates of IVF (16 estimates, OR = 1.65; 95% CI: 1.34-2.04; P < 0.0001), ICSI (24 estimates, OR = 1.31; 95% CI: 1.08-1.59; P = 0.0068), and mixed IVF + ICSI studies (16 estimates, OR = 2.37; 95% CI: 1.89-2.97; P < 0.0001) were also statistically significant. There is sufficient evidence in the existing literature suggesting that sperm DNA damage has a negative effect on clinical pregnancy following IVF and/or ICSI treatment.
authors.affiliation: Department of Surgery (Urology), University of Utah School of Medicine, Salt Lake City, UT, USA.
authors.forename: Luke
authors.initials: L
authors.lastname: Simon
authors.affiliation: Division of Urology, Department of Surgery, St. Mary's Hospital Center, St. Mary's Hospital, 3830 Lacombe Avenue, Montreal, Quebec H3T 1M5, Canada.
authors.forename: Armand
authors.initials: A
authors.lastname: Zini
authors.affiliation: Division of Urology, Department of Surgery, St. Mary's Hospital Center, St. Mary's Hospital, 3830 Lacombe Avenue, Montreal, Quebec H3T 1M5, Canada.
authors.forename: Alina
authors.initials: A
authors.lastname: Dyachenko
authors.affiliation: Division of Urology, Department of Surgery, St. Mary's Hospital Center, St. Mary's Hospital, 3830 Lacombe Avenue, Montreal, Quebec H3T 1M5, Canada.
authors.forename: Antonio
authors.initials: A
authors.lastname: Ciampi
authors.affiliation: Department of Surgery (Urology), University of Utah School of Medicine, Salt Lake City, UT, USA.|Department of Human Genetics, University of Utah School of Medicine, Salt Lake City, UT, USA.|Department of Obstetrics and Gynecology, University of Utah School of Medicine, Salt Lake City, UT, USA.
authors.forename: Douglas T
authors.initials: DT
authors.lastname: Carrell
mesh_terms: D020552:Comet Assay; D004249:DNA Damage; D053938:DNA Fragmentation; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D020287:In Situ Nick-End Labeling; D007248:Infertility, Male / Q000628:therapy*; D008297:Male; D011247:Pregnancy; D018873:Pregnancy Rate; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa / Q000378:metabolism*