title: Borrelia sp. phylogenetically different from Lyme disease- and relapsing fever-related Borrelia spp. in Amblyomma varanense from Python reticulatus.
abstract: Species of the genus Borrelia are causative agents of Lyme disease and relapsing fever. Lyme disease is the most commonly reported vector-borne disease in the northern hemisphere. However, in some parts of the world Lyme borreliosis and relapsing fever may be caused by novel Borrelia genotypes. Herein, we report the presence of a Borrelia sp. in an Amblyomma varanense collected from Python reticulatus.
  
  
  
  Ticks were collected from snakes, identified to species level and examined by PCR for the presence of Borrelia spp. flaB and 16S rRNA genes. Phylogenetic trees were constructed using the neighbour-joining method.
  
  
  
  Three A. varanense ticks collected from P. reticulatus were positive for a unique Borrelia sp., which was phylogenetically divergent from both Lyme disease- and relapsing fever-associated Borrelia spp.
  
  
  
  The results of this study suggest for the first time that there is a Borrelia sp. in A. varanense tick in the snake P. reticulatus that might be novel.
authors.affiliation: Biodiversity Research Cluster, Department of Biology, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok, 10400, Thailand.
authors.forename: Wachareeporn
authors.initials: W
authors.lastname: Trinachartvanit
authors.affiliation: Department of Biology, Faculty of Science, Ramkhamhaeng University, Ramkhamhaeng Road, Bangkok, 10240, Thailand.
authors.forename: Supanee
authors.initials: S
authors.lastname: Hirunkanokpun
authors.affiliation: Biodiversity Research Cluster, Department of Biology, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok, 10400, Thailand.
authors.forename: Ronnayuth
authors.initials: R
authors.lastname: Sudsangiem
authors.affiliation: Department of Science Education, Faculty of Science and Technology, Thepsatri Rajabhat University, Maung, Lopburi, 15000, Thailand.
authors.forename: Wanwisa
authors.initials: W
authors.lastname: Lijuan
authors.affiliation: Department of Biology, Faculty of Science and Technology, Thepsatri Rajabhat University, Maung, Lopburi, 15000, Thailand.
authors.forename: Duangjai
authors.initials: D
authors.lastname: Boonkusol
authors.affiliation: Biodiversity Research Cluster, Department of Biology, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok, 10400, Thailand.|Center of Excellence for Vectors and Vector-Borne Diseases, Faculty of Science, Mahidol University at Salaya, Phutthamonthon 4 Road, Nakhon Pathom, 73170, Thailand.
authors.forename: Visut
authors.initials: V
authors.lastname: Baimai
authors.affiliation: Biodiversity Research Cluster, Department of Biology, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok, 10400, Thailand. arunee.aha@mahidol.ac.th.|Center of Excellence for Vectors and Vector-Borne Diseases, Faculty of Science, Mahidol University at Salaya, Phutthamonthon 4 Road, Nakhon Pathom, 73170, Thailand. arunee.aha@mahidol.ac.th.
authors.forename: Arunee
authors.initials: A
authors.lastname: Ahantarig
mesh_terms: D000818:Animals; D017821:Boidae / Q000469:parasitology*; D001898:Borrelia / Q000235:genetics*; D026863:Ixodidae / Q000382:microbiology*; D008193:Lyme Disease / Q000382:microbiology*; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012061:Relapsing Fever / Q000382:microbiology*; D013984:Tick Infestations / Q000662:veterinary*
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: Amblyomma pattoni; Amblyomma varanense; Borrelia; Thailand; Tick