title: Viral Etiology Relationship between Human Papillomavirus and Human Breast Cancer and Target of Gene Therapy.
abstract: OBJECTIVE
  To explore the viral etiology of human breast cancer to determine whether there are novel molecular targets for gene therapy of breast cancer and provide evidence for the research of gene therapy and vaccine development for breast cancer.
  
  
  METHODS
  PCR was used to screen HPV16 and HPV18 oncogenes E6 and E7 in the SKBR3 cell line and in 76 paraffin embedded breast cancer tissue samples. RNA interference was used to knock down the expression of HPV18 E6 and E7 in SKBR3 cells, then the changes in the expression of cell-cycle related proteins, cell viability, colony formation, metastasis, and cell cycle progression were determined.
  
  
  RESULTS
  HPV18 oncogenes E6 and E7 were amplified and sequenced from the SKBR3 cells. Of the patient samples, 6.58% and 23.68% were tested to be positive for HPV18 E6 and HPV18 E7. In the cell culture models, the knockdown of HPV18 E6 and E7 inhibited the proliferation, metastasis, and cell cycle progression of SKBR3 cell. The knockdown also clearly affected the expression levels of cell cycle related proteins.
  
  
  CONCLUSIONS
  HPV was a contributor to virus caused human breast cancer, suggesting that the oncogenes in HPV were potential targets for gene therapy of breast cancer.
authors.affiliation: State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Collabrative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Beijing 100052, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Yan
authors.affiliation: State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Collabrative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Beijing 100052, China.
authors.forename: Zhi Ping
authors.initials: ZP
authors.lastname: Teng
authors.affiliation: Peking University People's Hospital, Beijing 100044, China.
authors.forename: Yun Xin
authors.initials: YX
authors.lastname: Chen
authors.affiliation: Peking University People's Hospital, Beijing 100044, China.
authors.forename: Dan Hua
authors.initials: DH
authors.lastname: Shen
authors.affiliation: College of Life Science and Bio-engineering, Beijing University of Technology, Beijing 100124, China.
authors.forename: Jin Tao
authors.initials: JT
authors.lastname: Li
authors.affiliation: State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Collabrative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Beijing 100052, China; College of Life Science and Bio-engineering, Beijing University of Technology, Beijing 100124, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zeng
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics / Q000628:therapy*; D005260:Female; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D008875:Middle Aged; D009856:Oncogene Proteins, Viral / Q000235:genetics* / Q000378:metabolism; D027383:Papillomaviridae / Q000502:physiology*; D030361:Papillomavirus Infections / Q000235:genetics / Q000628:therapy*; D016415:Sequence Alignment
chemical_list: D009856:Oncogene Proteins, Viral
keywords: Gene therapy; Human breast cancer; Human papillomavirus; RNA interference