title: Tumor Cell Detection by Mass Spectrometry Using Signal Ion Emission Reactive Release Amplification.
abstract: A method is presented for the detection of circulating tumor cells (CTC) using mass spectrometry (MS), through reporter-ion amplification. Particles functionalized with short-chain peptides are bound to cells through antibody-antigen interactions. Selective release and MS detection of peptides is shown to detect as few as 690 cells isolated from a 10 mL blood sample. Here we present proof-of-concept results that pave the way for further investigations.
authors.affiliation: Siemens Healthcare Diagnostics , 3400 Middlebury Street, Elkhart, Indiana 46516, United States.|Chemistry Department, Purdue University , 560 Oval Drive, West Lafayette, Indiana 47907-2084, United States.
authors.forename: Zane
authors.initials: Z
authors.lastname: Baird
authors.affiliation: Chemistry Department, Purdue University , 560 Oval Drive, West Lafayette, Indiana 47907-2084, United States.
authors.forename: Valentina
authors.initials: V
authors.lastname: Pirro
authors.affiliation: Chemistry Department, Purdue University , 560 Oval Drive, West Lafayette, Indiana 47907-2084, United States.
authors.forename: Stephen
authors.initials: S
authors.lastname: Ayrton
authors.affiliation: Chemistry Department, Purdue University , 560 Oval Drive, West Lafayette, Indiana 47907-2084, United States.
authors.forename: Adam
authors.initials: A
authors.lastname: Hollerbach
authors.affiliation: Siemens Healthcare Diagnostics , 3400 Middlebury Street, Elkhart, Indiana 46516, United States.
authors.forename: Cathleen
authors.initials: C
authors.lastname: Hanau
authors.affiliation: Siemens Healthcare Diagnostics , 3400 Middlebury Street, Elkhart, Indiana 46516, United States.
authors.forename: Karen
authors.initials: K
authors.lastname: Marfurt
authors.affiliation: Siemens Healthcare Diagnostics , 3400 Middlebury Street, Elkhart, Indiana 46516, United States.
authors.forename: Mary
authors.initials: M
authors.lastname: Foltz
authors.affiliation: Chemistry Department, Purdue University , 560 Oval Drive, West Lafayette, Indiana 47907-2084, United States.
authors.forename: R Graham
authors.initials: RG
authors.lastname: Cooks
authors.affiliation: Siemens Healthcare Diagnostics , 3400 Middlebury Street, Elkhart, Indiana 46516, United States.
authors.forename: Michael
authors.initials: M
authors.lastname: Pugia
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D002452:Cell Count; D045744:Cell Line, Tumor; D002469:Cell Separation / Q000379:methods*; D006801:Humans; D007158:Immunologic Techniques / Q000379:methods; D057230:Limit of Detection; D051379:Mice; D008970:Molecular Weight; D053758:Nanoparticles / Q000737:chemistry; D009360:Neoplastic Cells, Circulating / Q000276:immunology; D010455:Peptides / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D000906:Antibodies; D010455:Peptides; D012822:Silicon Dioxide