title: miR-483-3p regulates hyperglycaemia-induced cardiomyocyte apoptosis in transgenic mice.
abstract: Diabetic cardiomyopathy represents severe heart complications, and is the leading cause of morbidity and mortality among patients with diabetes. Although a few microRNAs (miRNAs) have been implicated in diabetes-related complications, a functional association between miRNAs and cardiac dysfunction in diabetic cardiomyopathy remains to be demonstrated. Our results show that miR-483-3p is upregulated in streptozotocin-induced diabetic mice, and cultured cardiomyocytes mimicking hyperglycemia. Overexpressing miR-483-3p in transgenic mice with diabetes mellitus (DM) exacerbated cardiomyocyte apoptosis by transcriptionally repressing insulin growth factor 1 (IGF1). Therefore, we have uncovered a novel signaling pathway, involving miR-483-3p-IGF1, that promotes myocardial cell apoptosis under high blood-glucose condition. Further, our study indicates that miR-483-3p could be a potential therapeutic target for managing diabetes-associated heart complications.
authors.affiliation: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China; Medical Science Institute of Hei Longjiang Province, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Qiao
authors.affiliation: Department of Biochemistry, Medical College of Qinghai University, Xining, Qinghai, China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China; Translational Medicine Center of Northern China, Harbin, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Ma
authors.affiliation: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China.
authors.forename: Chuxuan
authors.initials: C
authors.lastname: Wang
authors.affiliation: Institute of Clinical Pharmacy of the Second Affiliated Hospital of Harbin Medical University, China; The University Key Laboratory of Drug Research of Heilongjiang Province, China.
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Zou
authors.affiliation: Institute of Clinical Pharmacy of the Second Affiliated Hospital of Harbin Medical University, China; The University Key Laboratory of Drug Research of Heilongjiang Province, China.
authors.forename: Zhiyan
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Institute of Clinical Pharmacy of the Second Affiliated Hospital of Harbin Medical University, China; The University Key Laboratory of Drug Research of Heilongjiang Province, China.
authors.forename: Zhongqiu
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Han
authors.affiliation: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China.
authors.forename: Jun
authors.initials: J
authors.lastname: He
authors.affiliation: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Sun
authors.affiliation: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China.
authors.forename: Yicong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Pathophysiology, Harbin Medical University, Harbin, China.
authors.forename: Changqing
authors.initials: C
authors.lastname: Xu
authors.affiliation: Institute of Clinical Pharmacy of the Second Affiliated Hospital of Harbin Medical University, China; The University Key Laboratory of Drug Research of Heilongjiang Province, China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Du
authors.affiliation: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China; Medical Science Institute of Hei Longjiang Province, China. Electronic address: huanghuiqy@163.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Huang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D002460:Cell Line; D003921:Diabetes Mellitus, Experimental / Q000235:genetics; D006943:Hyperglycemia / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D035683:MicroRNAs / Q000235:genetics*; D032383:Myocytes, Cardiac / Q000473:pathology*; D051381:Rats; D013311:Streptozocin
chemical_list: D035683:MicroRNAs; C548913:Mirn483 microRNA, mouse; D013311:Streptozocin
keywords: Apoptosis; Diabetic cardiomyopathy; IGF1; MicroRNA