title: Biochanin-A antagonizes the interleukin-1β-induced catabolic inflammation through the modulation of NFκB cellular signaling in primary rat chondrocytes.
abstract: Biochanin-A, a phytoestrogen derived from herbal plants, protected from the IL-1β-induced loss of proteoglycans through the suppression of matrix degrading enzymes such as matrix metalloproteinase (MMP)-13, MMP-3, MMP-1, and ADAMTS-5 in primary rat chondrocytes and the knee articular cartilage. It also suppressed the expression of IL-1β-induced catabolic factors such as nitric oxide synthase 2, cyclooxygenase-2, prostaglandin E2, and inflammatory cytokines. Furthermore, biochanin-A suppressed the IL-1β-induced phosphorylation of NFκB, and inhibited its nuclear translocation in primary rat chondrocytes. These results indicate that biochanin-A antagonizes the IL-1β-induced catabolic effects through its anti-inflammatory activity that involves the modulation of NFκB signaling.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Chosun University, Gwangju, 61452, Republic of Korea.
authors.forename: Ji-Su
authors.initials: JS
authors.lastname: Oh
authors.affiliation: Department of Dental Bioengineering, Chosun University, Gwangju, 61452, Republic of Korea.
authors.forename: In-A
authors.initials: IA
authors.lastname: Cho
authors.affiliation: Department of Dental Bioengineering, Chosun University, Gwangju, 61452, Republic of Korea.
authors.forename: Kyeong-Rok
authors.initials: KR
authors.lastname: Kang
authors.affiliation: Department of Oral and Maxillofacial Surgery, Chosun University, Gwangju, 61452, Republic of Korea.
authors.forename: Jae-Seek
authors.initials: JS
authors.lastname: You
authors.affiliation: Department of Periodontology, Chosun University, Gwangju, 61452, Republic of Korea.
authors.forename: Sang-Joun
authors.initials: SJ
authors.lastname: Yu
authors.affiliation: Department of Prosthodontics, Chosun University, Gwangju, 61452, Republic of Korea.
authors.forename: Gyeong-Je
authors.initials: GJ
authors.lastname: Lee
authors.affiliation: Department of Oral and Maxillofacial Radiology, Chosun University, Gwangju, 61452, Republic of Korea.
authors.forename: Yo-Seob
authors.initials: YS
authors.lastname: Seo
authors.affiliation: Pre-Dentistry, School of Dentistry, Chosun University, Gwangju, 61452, Republic of Korea.
authors.forename: Chun Sung
authors.initials: CS
authors.lastname: Kim
authors.affiliation: Pre-Dentistry, School of Dentistry, Chosun University, Gwangju, 61452, Republic of Korea.
authors.forename: Do Kyung
authors.initials: DK
authors.lastname: Kim
authors.affiliation: Department of Oral and Maxillofacial Surgery, Chosun University, Gwangju, 61452, Republic of Korea.
authors.forename: Su-Gwan
authors.initials: SG
authors.lastname: Kim
authors.affiliation: Korea Basic Science Institute, Gwangju Center, Chonnam National University, Gwangju, 61186, Republic of Korea.
authors.forename: Young-Woo
authors.initials: YW
authors.lastname: Seo
authors.affiliation: Department of Biochemistry, Rush University Medical Center, Chicago, IL, 60612, USA.
authors.forename: Hee-Jeong
authors.initials: HJ
authors.lastname: Im
authors.affiliation: Pre-Dentistry, School of Dentistry, Chosun University, Gwangju, 61452, Republic of Korea. Electronic address: js_kim@chosun.ac.kr.
authors.forename: Jae-Sung
authors.initials: JS
authors.lastname: Kim
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects / Q000276:immunology*; D004305:Dose-Response Relationship, Drug; D019833:Genistein / Q000008:administration & dosage*; D053583:Interleukin-1beta / Q000276:immunology*; D008660:Metabolism / Q000187:drug effects; D016328:NF-kappa B / Q000276:immunology*; D010003:Osteoarthritis / Q000188:drug therapy* / Q000276:immunology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000276:immunology
chemical_list: D000893:Anti-Inflammatory Agents; D053583:Interleukin-1beta; D016328:NF-kappa B; D019833:Genistein; C004541:biochanin A
keywords: Anti-catabolic effects; Biochanin-A; Inflammation; Osteoarthritis; Phytoestrogen