title: Identification of a novel Aleutian mink disease virus B-cell epitope using a monoclonal antibody against VP2 protein.
abstract: Aleutian mink disease virus (AMDV) is a parvovirus that causes an immune complex-mediated disease in minks. Capsid protein VP2 is a major structural viral protein and can be used to diagnose AMDV. In this study, a specific monoclonal antibody, 1M13, was produced against the AMDV VP2 protein (amino acids 291-502). A linear VP2-protein epitope was identified by subjecting a series of partially overlapping synthesized peptides to be enzyme-linked immunosorbent assay (ELISA) analysis. The results indicated that (386)HLQQNFSTRYIYD(398) was the minimal linear epitope that could be recognized by mAb 1M13. ELISA assays revealed that mink anti-AMDV sera could also recognize the minimal linear epitope. Sequence alignments demonstrated that the linear epitope is highly conserved among AMDV strains except (386)H and is less conserved among Raccoon dog amdovirus, Gray fox amdovirus, Red fox amdovirus, Bat parvovirus and Mink enteritis parvovirus. Taken together, the generation of this VP2-specific mAb with a defined linear peptide epitope may have potential applications in the development of suitable diagnostic techniques for AMDV.
authors.affiliation: Institute of Special Wild Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, 4899 Juye Street, Changchun, Jilin Province, PR China. Electronic address: yili@caas.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Yi
authors.affiliation: Institute of Special Wild Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, 4899 Juye Street, Changchun, Jilin Province, PR China.
authors.forename: Yuening
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Institute of Special Wild Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, 4899 Juye Street, Changchun, Jilin Province, PR China.
authors.forename: Miao
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Institute of Special Wild Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, 4899 Juye Street, Changchun, Jilin Province, PR China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Cao
authors.affiliation: Institute of Special Wild Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, 4899 Juye Street, Changchun, Jilin Province, PR China.
authors.forename: Mingwei
authors.initials: M
authors.lastname: Tong
authors.affiliation: Institute of Special Wild Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, 4899 Juye Street, Changchun, Jilin Province, PR China. Electronic address: tcscsp@126.com.
authors.forename: Shipeng
authors.initials: S
authors.lastname: Cheng
authors.affiliation: Institute of Special Wild Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, 4899 Juye Street, Changchun, Jilin Province, PR China.
authors.forename: Xijun
authors.initials: X
authors.lastname: Yan
mesh_terms: D000453:Aleutian Mink Disease / Q000276:immunology / Q000821:virology; D000454:Aleutian Mink Disease Virus / Q000235:genetics / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000956:Antigens, Viral / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D018604:Epitope Mapping; D018985:Epitopes, B-Lymphocyte / Q000737:chemistry / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D017384:Sequence Deletion
chemical_list: D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D036022:Capsid Proteins; D018985:Epitopes, B-Lymphocyte; D011994:Recombinant Proteins; C442555:VP2 protein, Aleutian mink disease virus
keywords: Aleutian mink disease virus; B-cell epitope; Monoclonal antibody; VP2 protein