title: [Study on Molecular Biology of Rare Two A307 Phenotypes].
abstract: OBJECTIVE
  To identify the genotypes of the 2 blood samples whose serological typing were difficult by DNA sequencing analysis, and to investigate the molecular genetic basis of their genotypes.
  
  
  METHODS
  The 2 blood samples were preliminary genotyped by PCR-SSP. The complete exon 6 and 7 in the ABO genes were amplified by PCR and the PCR products were directly sequenced and clonal sequenced in order to identify the genotypes.
  
  
  RESULTS
  The forward typing showed that both samples were weak A, while the reverse typing showed that the samples contained anti-A1. They were preliminarily genotyped as A/O1.
  
  
  RESULTS
  The sequencing analysis showed that the 2 samples contained the nt467C>T and nt745C>T mutation in the A allele, which resulted in an amino acid change from Proline (Pro) to Leucine (Leu) at codon 156 and also from Arginine (Arg) to Tryptophan (Trp) at codon 249.
  
  
  CONCLUSIONS
  Through serology results and sequencing analysis, the 2 samples are identified as rare A307 phenotypes.
authors.affiliation: Qingdao Central Blood Center, Qingdao 266071, Shandong Province, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Han
authors.affiliation: Qingdao Central Blood Center, Qingdao 266071, Shandong Province, China.
authors.forename: Zhi-Hui
authors.initials: ZH
authors.lastname: Feng
authors.affiliation: Qingdao Central Hospital, Qingdao 266042, Shandong Province, China.
authors.forename: Qing-Ming
authors.initials: QM
authors.lastname: Guo
authors.affiliation: Qingdao Central Blood Center, Qingdao 266071, Shandong Province, China. E-mail: qd_hla@sina.com.
authors.forename: Ming-Min
authors.initials: MM
authors.lastname: Wang
mesh_terms: D000017:ABO Blood-Group System / Q000235:genetics*; D000483:Alleles; D004252:DNA Mutational Analysis; D005091:Exons; D005838:Genotype; D006801:Humans; D009154:Mutation; D010641:Phenotype; D016133:Polymerase Chain Reaction
chemical_list: D000017:ABO Blood-Group System