title: Circulating low IL-23: IL-35 cytokine ratio promotes progression associated with poor prognosisin breast cancer.
abstract: The interleukin (IL)-12 family, composed of heterodimeric cytokines including IL-12 (formed by IL-12p35 and IL-12p40 subunits), IL-23 (formed by IL-23p19 and IL-12p40 subunits), IL-27 (formed by IL-27p28 and EBI3 subunits) and IL-35 (formed by IL-12p35 and EBI3 subunits), establishes a link between innate and adaptive immunity that involves different immune effector cells and cytokines to tumors. However, the role of IL-12 family in breast cancer (BC) progression and prognosis remains unclear. In the present study, we demonstrated evidence indicating that EBI3, IL-12p35 and IL-12p40 but not IL-23p19 or IL-27p28 were highly expressed in BC tissues, suggested that tumor derived EBI3, IL-12p35 and IL-12p40 were associated with tumor progression. Circulating IL-12 and IL-23 low expressed, but IL-27 and IL-35 high expressed in BC patients, especially circulating IL-23 associated with IL-35 to mediate BC tumor resection. Ki-67, p53 and EGFR expression on BC tissues, as well as CA125, CA153 and CA199 levels on BC bloods increased when circulating IL-23: IL-35 ratio decreased. Together, for the first time, our data suggest that circulating IL-23: IL-35 ratio may be an important indicator association with BC progression and prognosis. However, further research should be carried out to assess the implications of circulating IL-23: IL-35 ratio in a larger sample size.
authors.affiliation: Department of Clinical Laboratory, Xiaolan Hospital of Southern Medical University Zhongshan 528415, China.
authors.forename: Guanghui
authors.initials: G
authors.lastname: Chen
authors.affiliation: Department of Pathology, Dongguan Hospital Affiliated to Medical College of Jinan University, The Fifth People's Hospital of Dongguan Dongguan 523808, China.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan Scientific Research Center, Guangdong Medical University Dongguan 523808, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Guan
authors.affiliation: Department of Clinical Laboratory, Xiaolan Hospital of Southern Medical University Zhongshan 528415, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.affiliation: Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan Scientific Research Center, Guangdong Medical University Dongguan 523808, China.
authors.forename: Qiankun
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan Scientific Research Center, Guangdong Medical University Dongguan 523808, China.
authors.forename: Bihua
authors.initials: B
authors.lastname: Lin
authors.affiliation: Department of Pathology, Dongguan Hospital Affiliated to Medical College of Jinan University, The Fifth People's Hospital of Dongguan Dongguan 523808, China.
authors.forename: Can
authors.initials: C
authors.lastname: Chen
authors.affiliation: Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan Scientific Research Center, Guangdong Medical University Dongguan 523808, China.
authors.forename: Mingyuan
authors.initials: M
authors.lastname: Huang
authors.affiliation: Department of Clinical Laboratory, Xiaolan Hospital of Southern Medical University Zhongshan 528415, China.
authors.forename: Jianan
authors.initials: J
authors.lastname: Chen
authors.affiliation: Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan Scientific Research Center, Guangdong Medical University Dongguan 523808, China.
authors.forename: Weiquan
authors.initials: W
authors.lastname: Wu
authors.affiliation: Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan Scientific Research Center, Guangdong Medical University Dongguan 523808, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan Scientific Research Center, Guangdong Medical University Dongguan 523808, China.
authors.forename: Keyuan
authors.initials: K
authors.lastname: Zhou
authors.affiliation: Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan Scientific Research Center, Guangdong Medical University Dongguan 523808, China.
authors.forename: Jincheng
authors.initials: J
authors.lastname: Zeng
keywords: Breast cancer; IL-23; IL-27; IL-35; interleukin (IL)-12