title: Evidence for a "metabolically inactive" inorganic phosphate pool in adenosine triphosphate synthase reaction using localized 31P saturation transfer magnetic resonance spectroscopy in the rat brain at 11.7 T.
abstract: With the increased spectral resolution made possible at high fields, a second, smaller inorganic phosphate resonance can be resolved on (31)P magnetic resonance spectra in the rat brain. Saturation transfer was used to estimate de novo adenosine triphosphate synthesis reaction rate. While the main inorganic phosphate pool is used by adenosine triphosphate synthase, the second pool is inactive for this reaction. Accounting for this new pool may not only help us understand (31)P magnetic resonance spectroscopy metabolic profiles better but also better quantify adenosine triphosphate synthesis.
authors.affiliation: Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), Direction de la Recherche Fondamentale (DRF), Institut d'Imagerie Biomédicale (I2BM), Molecular Imaging Research Center (MIRCen), Fontenay-aux-Roses, France Centre National de la Recherche Scientifique (CNRS), Université Paris-Saclay, UMR 9199, Neurodegenerative Diseases Laboratory, Fontenay-aux-Roses, France brice.tiret@cea.fr.
authors.forename: Brice
authors.initials: B
authors.lastname: Tiret
authors.affiliation: Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), Direction de la Recherche Fondamentale (DRF), Institut d'Imagerie Biomédicale (I2BM), Molecular Imaging Research Center (MIRCen), Fontenay-aux-Roses, France Centre National de la Recherche Scientifique (CNRS), Université Paris-Saclay, UMR 9199, Neurodegenerative Diseases Laboratory, Fontenay-aux-Roses, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Brouillet
authors.affiliation: Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), Direction de la Recherche Fondamentale (DRF), Institut d'Imagerie Biomédicale (I2BM), Molecular Imaging Research Center (MIRCen), Fontenay-aux-Roses, France Centre National de la Recherche Scientifique (CNRS), Université Paris-Saclay, UMR 9199, Neurodegenerative Diseases Laboratory, Fontenay-aux-Roses, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Valette
mesh_terms: D025181:ATP Synthetase Complexes / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D001923:Brain Chemistry; D004734:Energy Metabolism / Q000502:physiology; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D010710:Phosphates / Q000502:physiology*; D010759:Phosphorus Isotopes; D051381:Rats
chemical_list: D010710:Phosphates; D010759:Phosphorus Isotopes; D000255:Adenosine Triphosphate; D025181:ATP Synthetase Complexes
keywords: Mathematical modelling; adenosine triphosphate; energy metabolism; magnetic resonance spectroscopy; pH