title: The Organo- and Cytoprotective Effects of Heat-shock Protein in Response to Injury Due to Radiofrequency Ablation in Rat Liver.
abstract: OBJECTIVE
  In treating liver tumors, preserving hepatic reserve and reducing surgical invasiveness are important for minimizing postoperative complications. Geranylgeranylacetone (GGA) is reported to selectively induce heat-shock protein 70 (HSP70), which initiates a powerful cytoprotective effect. We investigated the function of HSP70 under conditions of radiofrequency ablation (RFA) of the liver.
  
  
  METHODS
  Male Wistar rats were divided into three groups: a control group, a group administered GGA, and a group administered GGA plus quercetin, an HSP70 synthesis inhibitor. Expression of HSP70 and heat-shock factor-1 (HSF1) in the liver was measured at the protein level, and severity of liver damage was investigated using serum and hepatic tissue.
  
  
  RESULTS
  The GGA-treated group had higher expression of HSP70 and HSF1 than the other groups. Peak liver damage in all groups occurred 6 h after RFA. The GGA-treated group also had significantly less liver damage and lower serum level of the inflammatory cytokine tumor necrosis factor-α, and a lower rate of apoptosis in tissue around post-ablation necrosis. Expression of HSP70 and HSF1 was suppressed in the group treated with GGA and quercetin, and this group had severe liver damage.
  
  
  CONCLUSIONS
  Induction of HSP in the liver by GGA may be applicable in future treatments for hepatocellular carcinoma or liver metastasis. The present findings suggest that if preoperative administration of GGA can offer protective effects in the liver, treatment options could be increased and liver failure and other complications might be avoided.
authors.affiliation: Department of Surgery, School of Medicine, Kurume University, Kurume, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kawashima
authors.affiliation: Department of Surgery, School of Medicine, Kurume University, Kurume, Japan thisaka@med.kurume-u.ac.jp.
authors.forename: Toru
authors.initials: T
authors.lastname: Hisaka
authors.affiliation: Department of Surgery, School of Medicine, Kurume University, Kurume, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Horiuchi
authors.affiliation: Department of Surgery, School of Medicine, Kurume University, Kurume, Japan.
authors.forename: Hiroto
authors.initials: H
authors.lastname: Ishikawa
authors.affiliation: Department of Surgery, School of Medicine, Kurume University, Kurume, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Uchida
authors.affiliation: Department of Surgery, School of Medicine, Kurume University, Kurume, Japan.
authors.forename: Tetsushi
authors.initials: T
authors.lastname: Kinugasa
authors.affiliation: Department of Surgery, School of Medicine, Kurume University, Kurume, Japan.
authors.forename: Daimei
authors.initials: D
authors.lastname: Etou
authors.affiliation: Department of Surgery, School of Medicine, Kurume University, Kurume, Japan.
authors.forename: Kouji
authors.initials: K
authors.lastname: Okuda
authors.affiliation: Department of Surgery, School of Medicine, Kurume University, Kurume, Japan.
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Akagi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000378:metabolism; D004224:Diterpenes / Q000494:pharmacology; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D000076249:Heat Shock Transcription Factors; D008099:Liver / Q000378:metabolism* / Q000473:pathology / Q000528:radiation effects; D008297:Male; D065840:Protective Factors; D011794:Quercetin / Q000494:pharmacology; D011833:Radiation Injuries, Experimental / Q000378:metabolism*; D016634:Radiosurgery / Q000009:adverse effects*; D017208:Rats, Wistar; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D000975:Antioxidants; D004268:DNA-Binding Proteins; D004224:Diterpenes; D018840:HSP70 Heat-Shock Proteins; D000076249:Heat Shock Transcription Factors; C000619709:Hsf1 protein, rat; D014157:Transcription Factors; D011794:Quercetin; C031049:geranylgeranylacetone
keywords: Heat-shock protein; geranylgeranylacetone; radiofrequency ablation