title: CRMP4 regulates dendritic growth and maturation via the interaction with actin cytoskeleton in cultured hippocampal neurons.
abstract: CRMP family proteins (CRMPs) are critical for neurite outgrowth and maturation in the developing nervous system. However, the distinct roles of CRMP isoforms remain to be elucidated, especially in dendritic development. Here, we show that CRMP4 is sufficient and necessary for dendritic growth and maturation in cultured hippocampal neurons. Overexpression of CRMP4 promotes and genetic knockdown of CRMP4 inhibits the amount of dendritic tips, total dendritic length, spine density, and the frequency but not amplitude of miniature excitatory synaptic current. By GST-pulldown assay, we reveal that CRMP4 interacts with actin cytoskeleton by its C-terminal region, but not by N-terminal. Overexpression of actin-interacting region of CRMP4 promoted dendritic growth and maturation as CRMP4 wildtype. Taken together, these results suggest that CRMP4 is involved in dendritic development via the interaction with actin cytoskeleton in hippocampal neurons.
authors.affiliation: Department of Pediatrics, the First Affiliated Hospital of Jinan University, Guangzhou 510630, China; Department of Pediatrics, Guangzhou Women and Children's Medical Center, Guangzhou, 510120, China; Department of Anatomy, Medical College of Jinan University, Guangzhou 510630, China.
authors.forename: Caihui
authors.initials: C
authors.lastname: Cha
authors.affiliation: Department of Anatomy, Medical College of Jinan University, Guangzhou 510630, China.
authors.forename: Jifeng
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Anatomy, Medical College of Jinan University, Guangzhou 510630, China; Department of Orthopedics, the First Affiliated Hospital of Jinan University, Guangzhou 510630, China.
authors.forename: Zhisheng
authors.initials: Z
authors.lastname: Ji
authors.affiliation: Department of Anatomy, Medical College of Jinan University, Guangzhou 510630, China; Department of Orthopedics, the First Affiliated Hospital of Jinan University, Guangzhou 510630, China.
authors.forename: Minghui
authors.initials: M
authors.lastname: Tan
authors.affiliation: Department of Anatomy, Medical College of Jinan University, Guangzhou 510630, China.
authors.forename: Sumei
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Anatomy, Medical College of Jinan University, Guangzhou 510630, China.
authors.forename: Fengming
authors.initials: F
authors.lastname: Wu
authors.affiliation: Department of Anatomy, Medical College of Jinan University, Guangzhou 510630, China; Department of Neurosurgery, the First Affiliated Hospital of Jinan University, Guangzhou 510630, China.
authors.forename: Keen
authors.initials: K
authors.lastname: Chen
authors.affiliation: Department of Pediatrics, Guangzhou Women and Children's Medical Center, Guangzhou, 510120, China.
authors.forename: Sitang
authors.initials: S
authors.lastname: Gong
authors.affiliation: Department of Anatomy, Medical College of Jinan University, Guangzhou 510630, China. Electronic address: tgqguo@yahoo.com.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Guo
authors.affiliation: Department of Orthopedics, the First Affiliated Hospital of Jinan University, Guangzhou 510630, China. Electronic address: tlinhsh@jnu.edu.cn.
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Lin
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D003712:Dendrites / Q000502:physiology*; D000076122:Disks Large Homolog 4 Protein; D019706:Excitatory Postsynaptic Potentials / Q000235:genetics / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006624:Hippocampus / Q000166:cytology*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000166:cytology*; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014162:Transfection
chemical_list: D000076122:Disks Large Homolog 4 Protein; C501449:Dlg4 protein, rat; C449760:Dpysl3 protein, rat; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D034741:RNA, Small Interfering; D049452:Green Fluorescent Proteins
keywords: Actin; CRMP4; Dendritic development; Hippocampal neuron; Spine; mEPSC