title: "Self-cleaving" 2A peptide from porcine teschovirus-1 mediates cleavage of dual fluorescent proteins in transgenic Eimeria tenella.
abstract: The "self-cleaving" 2A sequence of picornavirus, which mediates ribosome-skipping events, enables the generation of two or more separate peptide products from one mRNA containing one or more "self-cleaving" 2A sequences. In this study, we introduced a single 2A sequence of porcine teschovirus-1 (P2A) linked to two fluorescent protein genes, the enhanced yellow fluorescent protein (EYFP) gene and the red fluorescent protein (RFP) gene, in a single cassette into transgenic Eimeria tenella (EtER). As expected, we obtained two separated protein molecules rather than a fused protein, although the two molecules were translated from the same mRNA carrying a single "self-cleaving" 2A sequence. Importantly, RFP led by a secretion signal was secreted into parasitophorous vacuoles, while EYFP localized mainly to the nucleus of EtER. Our results demonstrate that the "self-cleaving" 2A sequence actively mediated cleavage of polyproteins in the apicomplexan parasite E. tenella.
authors.affiliation: State Key Laboratory of Agrobiotechnology and College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.|National Animal Protozoa Laboratory and College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.
authors.forename: Xinming
authors.initials: X
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Agrobiotechnology and College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.|National Animal Protozoa Laboratory and College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.|Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, China Agricultural University, Beijing, 100193, China.
authors.forename: Xianyong
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Agrobiotechnology and College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.|National Animal Protozoa Laboratory and College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.
authors.forename: Geru
authors.initials: G
authors.lastname: Tao
authors.affiliation: National Animal Protozoa Laboratory and College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Qin
authors.affiliation: National Animal Protozoa Laboratory and College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.
authors.forename: Guangwen
authors.initials: G
authors.lastname: Yin
authors.affiliation: State Key Laboratory of Agrobiotechnology and College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.|National Animal Protozoa Laboratory and College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.
authors.forename: Jingxia
authors.initials: J
authors.lastname: Suo
authors.affiliation: State Key Laboratory of Agrobiotechnology and College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China. suoxun@cau.edu.cn.|National Animal Protozoa Laboratory and College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China. suoxun@cau.edu.cn.|Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, China Agricultural University, Beijing, 100193, China. suoxun@cau.edu.cn.
authors.forename: Xun
authors.initials: X
authors.lastname: Suo
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002645:Chickens / Q000469:parasitology; D016786:Eimeria tenella / Q000235:genetics* / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics* / Q000378:metabolism; D030781:Organisms, Genetically Modified / Q000235:genetics / Q000378:metabolism; D052700:Teschovirus / Q000235:genetics* / Q000378:metabolism; D000097573:Red Fluorescent Protein
chemical_list: D001426:Bacterial Proteins; D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein; C054562:yellow fluorescent protein, Bacteria