title: GOLDEN2-LIKE transcription factors coordinate the tolerance to Cucumber mosaic virus in Arabidopsis.
abstract: Arabidopsis thaliana GOLDEN2-LIKE (GLKs) transcription factors play important roles in regulation of photosynthesis-associated nuclear genes, as well as participate in chloroplast development. However, the involvement of GLKs in plants resistance to virus remains largely unknown. Here, the relationship between GLKs and Cucumber mosaic virus (CMV) stress response was investigated. Our results showed that the Arabidopsis glk1glk2 double-mutant was more susceptible to CMV infection and suffered more serious damages (such as higher oxidative damages, more compromised in PSII photochemistry and more reactive oxygen species accumulation) when compared with the wild-type plants. Interestingly, there was little difference between single mutant (glk1 or glk2) and wild-type plants in response to CMV infection, suggesting GLK1 and GLK2 might function redundant in virus resistance in Arabidopsis. Furthermore, the induction of antioxidant system and defense-associated genes expression in the double mutant were inhibited when compared with single mutant or wild-type plants after CMV infection. Further evidences showed that salicylic acid (SA) and jasmonic acid (JA) might be involved in GLKs-mediated virus resistance, as SA or JA level and synthesis-related genes transcription were impaired in glk1glk2 mutant. Taken together, our results indicated that GLKs played a positively role in virus resistance in Arabidopsis.
authors.affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu 610064, China.
authors.forename: Xue-Ying
authors.initials: XY
authors.lastname: Han
authors.affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu 610064, China.
authors.forename: Peng-Xu
authors.initials: PX
authors.lastname: Li
authors.affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu 610064, China.
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Zou
authors.affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu 610064, China.
authors.forename: Wen-Rong
authors.initials: WR
authors.lastname: Tan
authors.affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu 610064, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zheng
authors.affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu 610064, China. Electronic address: yuanmiao1892@163.com.
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Zhang
authors.affiliation: Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, State Key Laboratory of Hydraulics and Mountain River Engineering, Sichuan University, Chengdu 610064, China. Electronic address: hhlin@scu.edu.cn.
authors.forename: Hong-Hui
authors.initials: HH
authors.lastname: Lin
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology*; D017360:Arabidopsis / Q000378:metabolism / Q000502:physiology* / Q000821:virology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000502:physiology*; D017799:Cucumovirus / Q000472:pathogenicity*; D003517:Cyclopentanes / Q000378:metabolism; D018384:Oxidative Stress; D054883:Oxylipins / Q000378:metabolism; D010788:Photosynthesis; D012333:RNA, Messenger / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D020156:Salicylic Acid / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*
chemical_list: D029681:Arabidopsis Proteins; D003517:Cyclopentanes; C468867:GLK1 protein, Arabidopsis; D054883:Oxylipins; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D014157:Transcription Factors; C011006:jasmonic acid; D020156:Salicylic Acid
keywords: Arabidopsis; CMV; GLKs; JA; SA; Virus resistance