title: miR-199b-5p modulates BMSC osteogenesis via suppressing GSK-3β/β-catenin signaling pathway.
abstract: miR-199b-5p is up-regulated significantly during the osteogenesis process in human bone marrow stromal cells (BMSCs). Inhibiting miR-199b-5p notably reduces while over-expressing miR-199b-5p promotes the BMSCs osteoblast differentiation, suggested by the alternations of osteogenic genes expression, ALP activity and the ARS-stained mineral nodules. miR-199b-5p exerts its role in BMSC osteogenesis most probably through the GSK-3β/β-catenin signaling pathway. In conclusion, the present study revealed for the first time that miR-199b-5p plays a positive role in osteoblast differentiation.
authors.affiliation: Department of Orthopedics, Xiangya Hospital Central South University, PR China.
authors.forename: Ruibo
authors.initials: R
authors.lastname: Zhao
authors.affiliation: Department of Orthopedics, Xiangya Hospital Central South University, PR China.
authors.forename: Yusheng
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Orthopedics, Xiangya Hospital Central South University, PR China.
authors.forename: Zhangyuan
authors.initials: Z
authors.lastname: Lin
authors.affiliation: Department of Orthopedics, Xiangya Hospital Central South University, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wan
authors.affiliation: Department of Orthopedics, Xiangya Hospital Central South University, PR China.
authors.forename: Can
authors.initials: C
authors.lastname: Xu
authors.affiliation: Department of Orthopedics, Xiangya Hospital Central South University, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: Department of Orthopedics, Xiangya Hospital Central South University, PR China. Electronic address: doczhu2003@aliyun.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D000328:Adult; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D015536:Down-Regulation / Q000502:physiology; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology; D000071679:Glycogen Synthase Kinase 3 beta / Q000378:metabolism; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology*; D035683:MicroRNAs / Q000378:metabolism*; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology; D051176:beta Catenin / Q000378:metabolism
chemical_list: C495270:CTNNB1 protein, human; D035683:MicroRNAs; D051176:beta Catenin; C531408:mirn199 microRNA, human; D000071679:Glycogen Synthase Kinase 3 beta
keywords: BMSCs; GSK-3β; Osteogenesis; miR-199b-5p; β-catenin