title: Factors affecting the success of a large embryo transfer program in Holstein cattle in a commercial herd in the southeast region of the United States.
abstract: The objectives of this study were to evaluate factors affecting in vivo embryo production and pregnancy per embryo transfer (P/ET) in Holstein cattle in the southeast region of the United States. Data from a total of 516 embryo collections and 10,297 ETs performed from 2011 to 2014 were available. For embryo production, the effects of donor parity (nulliparous [N], primiparous [P], multiparous [M]), average temperature-humidity index (THI) at embryo collection, days in milk at embryo collection, occurrence of calving problems, and occurrence of metritis postpartum were evaluated. For P/ET, the effects of donor parity (N or parous), recipient parity (N, P, and M), embryo type (fresh, frozen, IVF, and IVF-frozen), embryo developmental stage (4-7), embryo quality (1-3), recipient estrous cycle day at ET (6-9), average THI at ET, days in milk at ET, milk yield at ET, occurrence of calving problems (abortion, dystocia, twins, fetal death, or retained placenta), and occurrence of metritis postpartum were evaluated. Pregnancy was diagnosed at 41 ± 3 days of gestation. Continuous and binary data were analyzed using the MIXED and GLIMMIX procedures of SAS, respectively. Parity affected embryo production; M had greater number and percentage of unfertilized embryos and lesser percentage of viable embryos than P and N. Recipient parity, embryo type, embryo stage, embryo quality, estrous cycle day at ET, and THI at ET affected P/ET. There was an interaction between recipient parity and THI at ET. P/ET was greater for N than P and greater for P than M, greater for fresh embryos than others, greater for stage 7 than others, greater for quality 1 than 2 and greater for quality 2 than 3, and greater for ET on estrous cycle Day 7 and 8 than 6. P/ET was decreased for THI ≥80 in N and THI ≥72 in P and M. Calving problems and metritis also affected P/ET in P and M and was lesser for cows that had calving problems and metritis. In conclusion, embryo production was affected by donor parity, and P/ET was affected by embryo type, embryo stage, embryo quality, recipient estrous cycle day at ET, THI, calving problems, and metritis.
authors.affiliation: Escola de Medicina Veterinária, Universidade Federal da Bahia, Salvador, BA, Brazil.
authors.forename: P A
authors.initials: PA
authors.lastname: Ferraz
authors.affiliation: Southern Embryo, Athens, Georgia, USA.
authors.forename: C
authors.initials: C
authors.lastname: Burnley
authors.affiliation: North Florida Holsteins, Bell, Florida, USA.
authors.forename: J
authors.initials: J
authors.lastname: Karanja
authors.affiliation: Department of Animal Sciences, University of Florida, Gainesville, Florida, USA.
authors.forename: A
authors.initials: A
authors.lastname: Viera-Neto
authors.affiliation: Department of Animal Sciences, University of Florida, Gainesville, Florida, USA.
authors.forename: J E P
authors.initials: JE
authors.lastname: Santos
authors.affiliation: Department of Animal Sciences, University of Florida, Gainesville, Florida, USA; Department of Large Animal Clinical Sciences, University of Florida, Gainesville, Florida, USA.
authors.forename: R C
authors.initials: RC
authors.lastname: Chebel
authors.affiliation: Department of Large Animal Clinical Sciences, University of Florida, Gainesville, Florida, USA; D. H. Barron Reproductive and Perinatal Biology Research Program, University of Florida, Gainesville, Florida, USA. Electronic address: galvaok@ufl.edu.
authors.forename: K N
authors.initials: KN
authors.lastname: Galvão
mesh_terms: D000818:Animals; D002417:Cattle / Q000196:embryology* / Q000502:physiology; D002418:Cattle Diseases / Q000209:etiology; D004420:Dystocia; D046149:Embryo Culture Techniques / Q000662:veterinary; D004624:Embryo Transfer / Q000662:veterinary*; D004716:Endometritis / Q000662:veterinary; D005260:Female; D015999:Multivariate Analysis; D011247:Pregnancy; D011256:Pregnancy Outcome; D012189:Retrospective Studies; D020858:Tissue and Organ Harvesting / Q000662:veterinary; D014481:United States
keywords: Dairy cattle; Embryo transfer; Pregnancy