title: Regulation of the Na+/K+-ATPase Ena1 Expression by Calcineurin/Crz1 under High pH Stress: A Quantitative Study.
abstract: Regulated expression of the Ena1 Na+-ATPase is a crucial event for adaptation to high salt and/or alkaline pH stress in the budding yeast Saccharomyces cerevisiae. ENA1 expression is under the control of diverse signaling pathways, including that mediated by the calcium-regulatable protein phosphatase calcineurin and its downstream transcription factor Crz1. We present here a quantitative study of the expression of Ena1 in response to alkalinization of the environment and we analyze the contribution of Crz1 to this response. Experimental data and mathematical models substantiate the existence of two stress-responsive Crz1-binding sites in the ENA1 promoter and estimate that the contribution of Crz1 to the early response of the ENA1 promoter is about 60%. The models suggest the existence of a second input with similar kinetics, which would be likely mediated by high pH-induced activation of the Snf1 kinase.
authors.affiliation: Institut de Biotecnologia i Biomedicina, Universitat Autònoma de Barcelona, Cerdanyola del Vallès 08193, Barcelona, Spain.|Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Cerdanyola del Vallès 08193, Barcelona, Spain.
authors.forename: Silvia
authors.initials: S
authors.lastname: Petrezsélyová
authors.affiliation: Institut de Biotecnologia i Biomedicina, Universitat Autònoma de Barcelona, Cerdanyola del Vallès 08193, Barcelona, Spain.|Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Cerdanyola del Vallès 08193, Barcelona, Spain.
authors.forename: María
authors.initials: M
authors.lastname: López-Malo
authors.affiliation: Institut de Biotecnologia i Biomedicina, Universitat Autònoma de Barcelona, Cerdanyola del Vallès 08193, Barcelona, Spain.|Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Cerdanyola del Vallès 08193, Barcelona, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Canadell
authors.affiliation: Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Cerdanyola del Vallès 08193, Barcelona, Spain.
authors.forename: Alicia
authors.initials: A
authors.lastname: Roque
authors.affiliation: Institut de Biotecnologia i Biomedicina, Universitat Autònoma de Barcelona, Cerdanyola del Vallès 08193, Barcelona, Spain.|Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Cerdanyola del Vallès 08193, Barcelona, Spain.
authors.forename: Albert
authors.initials: A
authors.lastname: Serra-Cardona
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas (IBMCP), Universitat Politècnica de València-Consejo Superior de Investigaciones Científicas, Valencia, 46022, Spain.
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Marqués
authors.affiliation: IRB Lleida, Universitat de Lleida, Lleida 25198, Spain.|Universitat de Lleida, Lleida 25198, Spain.
authors.forename: Ester
authors.initials: E
authors.lastname: Vilaprinyó
authors.affiliation: IRB Lleida, Universitat de Lleida, Lleida 25198, Spain.|Universitat de Lleida, Lleida 25198, Spain.
authors.forename: Rui
authors.initials: R
authors.lastname: Alves
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas (IBMCP), Universitat Politècnica de València-Consejo Superior de Investigaciones Científicas, Valencia, 46022, Spain.
authors.forename: Lynne
authors.initials: L
authors.lastname: Yenush
authors.affiliation: Institut de Biotecnologia i Biomedicina, Universitat Autònoma de Barcelona, Cerdanyola del Vallès 08193, Barcelona, Spain.|Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, Cerdanyola del Vallès 08193, Barcelona, Spain.
authors.forename: Joaquín
authors.identifier: http://orcid.org/0000-0002-6774-2987
authors.initials: J
authors.lastname: Ariño
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000235:genetics; D001665:Binding Sites / Q000235:genetics; D019703:Calcineurin / Q000378:metabolism / Q000502:physiology*; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D006863:Hydrogen-Ion Concentration; D030781:Organisms, Genetically Modified; D011401:Promoter Regions, Genetic; D021381:Protein Transport; D050436:Regulatory Elements, Transcriptional; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics* / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism / Q000502:physiology*
chemical_list: C109868:CRZ1 protein, S cerevisiae; D004268:DNA-Binding Proteins; C086375:ENA1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D019703:Calcineurin; D000254:Sodium-Potassium-Exchanging ATPase