title: Quantifying Serum Level of Glycochenodeoxycholic Acid Using High Performance Liquid Chromatography in Obstructive Jaundice Patients.
abstract: Introduction. Accumulation of glycochenodeoxycholic acid (GCDC) in serum has a clinical significance as an inductor of pathological hepatocyte apoptosis, which impairs liver function. Inhibition of GCDC accumulation can be used as a marker in therapy. This study was aimed to quantify the serum level of GCDC in obstructive jaundice patients. Methodology. GCDC acid level in the serum was quantified using high performance liquid chromatography (HPLC) technique according to Muraca and Ghoos modified method. It was performed before and after decompression at day 7 and day 14. The sample was extracted with solid phase extraction (SPE) technique on SPE column. The results were analyzed using SPSS V 16.0 (P < 0.05) and quantified with standard curve on GCDC acid. Result. There were 21 cases with range of GCDC acid serum level before decompression was 90.9 (SD 205.5) μmol/L and day 7 after decompression decreased to 4.0 (SD 46.4) μmol/L and then increased to 11.3 (SD 21.9) μmol/L (P < 0.05). This method could separate GCDC acid on serum with good resolution, high precision and accuracy, and linear calibration curve on measured level range. Conclusion. HPLC can quantify GCDC acid serum on obstructive jaundice patients and can be used to support its pharmacokinetic study.
authors.affiliation: Digestive Surgery Division, Cipto Mangunkusumo Hospital and Faculty of Medicine, University of Indonesia, Jakarta 10430, Indonesia; Department of Surgery, Faculty of Medicine, University of Indonesia, Medical Staff Building 4th Floor, Jalan Diponegoro No. 71, Jakarta 10430, Indonesia.
authors.forename: Toar J M
authors.initials: TJ
authors.lastname: Lalisang
authors.affiliation: Department of Pharmacology & Therapeutic, Faculty of Medicine, University of Indonesia, Jakarta 10430, Indonesia.
authors.forename: Metta Sinta
authors.initials: MS
authors.lastname: Sari Wiria
authors.affiliation: Department of Pharmacology & Therapeutic, Faculty of Medicine, University of Indonesia, Jakarta 10430, Indonesia.
authors.forename: Vivian
authors.initials: V
authors.lastname: Sutikno
authors.affiliation: Department of Pharmacology & Therapeutic, Faculty of Medicine, University of Indonesia, Jakarta 10430, Indonesia.
authors.forename: Melva
authors.initials: M
authors.lastname: Louisa
authors.affiliation: Department of Pharmacology & Therapeutic, Faculty of Medicine, University of Indonesia, Jakarta 10430, Indonesia.
authors.forename: Arie
authors.initials: A
authors.lastname: Estuningtyas