title: Detection of antibiotic-resistant bacteria endowed with antimicrobial activity from a freshwater lake and their phylogenetic affiliation.
abstract: Antimicrobial resistance poses a serious challenge to global public health. In this study, fifty bacterial strains were isolated from the sediments of a freshwater lake and were screened for antibiotic resistance. Out of fifty isolates, thirty-three isolates showed resistance against at least two of the selected antibiotics. Analysis of 16S rDNA sequencing revealed that the isolates belonged to ten different genera, namely Staphylococcus(n = 8), Bacillus(n = 7), Lysinibacillus(n = 4), Achromobacter(n=3), bacterium(n = 3), Methylobacterium(n = 2), Bosea(n = 2), Aneurinibacillus(n = 2), Azospirillum(n = 1), Novosphingobium(n = 1). Enterobacterial repetitive intergenic consensus (ERIC) and BOX-PCR markers were used to study the genetic relatedness among the antibiotic resistant isolates. Further, the isolates were screened for their antimicrobial activity against bacterial pathogens viz., Staphylococcus aureus(MTCC-96), Pseudomonas aeruginosa(MTCC-2453) and Escherichia coli(MTCC-739), and pathogenic fungi viz., Fusarium proliferatum (MTCC-286), Fusarium oxysporum (CABI-293942) and Fusarium oxy. ciceri (MTCC-2791). In addition, biosynthetic genes (polyketide synthase II (PKS-II) and non-ribosomal peptide synthetase (NRPS)) were detected in six and seven isolates, respectively. This is the first report for the multifunctional analysis of the bacterial isolates from a wetland with biosynthetic potential, which could serve as potential source of useful biologically active metabolites.
authors.affiliation: Department of Biotechnology, Mizoram University , Aizawl , Mizoram , India.
authors.lastname: Zothanpuia
authors.affiliation: Department of Biotechnology, Mizoram University , Aizawl , Mizoram , India.
authors.forename: Ajit K
authors.initials: AK
authors.lastname: Passari
authors.affiliation: Molecular Glyco-biotechnology Group, University of Ireland , Galway , Ireland , UK.
authors.forename: Vijai K
authors.initials: VK
authors.lastname: Gupta
authors.affiliation: Department of Biotechnology, Mizoram University , Aizawl , Mizoram , India.
authors.forename: Bhim P
authors.initials: BP
authors.lastname: Singh
keywords: 16S rRNA gene; Antibiotic susceptibility; BOX-PCR; ERIC-PCR; NRPS; PKS II