title: In vitro assessment of the biological response of Ti6Al4V implants coated with hydroxyapatite microdomains.
abstract: Dental implantology is still an expanding field of scientific study because of the number of people that receive dental therapies throughout their lives worldwide. Recovery times associated to dental surgery are still long and demand strategies to improve integration of metallic devices with hard tissues. In this work, an in vitro ceramic coating is proposed to improve and accelerate osseointegration of titanium surfaces conceived to be used as dental implants or hip or knee prosthesis, shaped either as dishes or screws. Such coating consists of hydroxyapatite microdomains on the implant surfaces obtained in vitro by immersion of titanium alloy samples (Ti6Al4V) in a simulated body fluid. This titanium alloy is highly used in implant dentistry and trauma surgery, among other fields. Once the immersion times under physiological conditions yielding to different ceramic topographies on this alloy were set, the acellular coating time of major interest so as to optimize its biological development was determined. For this purpose, dental pulp mesenchymal cells were cultured on titanium coated surfaces with different hydroxyapatite outline, and cell adhesion, proliferation and morphology were followed through histological techniques and scanning electron microscopy. It was found that 4 days of acellular hydroxyapatite coating led to a significant cell adhesion on the titanium alloys at an early stage (6 h). Cells tended although to detach from the surface of the coating over time, but those adhered on domains of intricated topography or hydroxyapatite cauliflowers proliferated on them, leading to isolated large cell clusters. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2723-2729, 2016.
authors.affiliation: Centre for Biomaterials and Tissue Engineering, Universitat Politècnica De València, Spain.|Biomedical Research Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Valencia, Spain.
authors.forename: R Salvador
authors.initials: RS
authors.lastname: Clavell
authors.affiliation: Department of D'Anatomia Patològica, Facultat De Medicina I Odontologia, Universitat De València and INCLIVA, Spain.
authors.forename: J J Martín
authors.initials: JJ
authors.lastname: de Llano
authors.affiliation: Department of D'Anatomia Patològica, Facultat De Medicina I Odontologia, Universitat De València and INCLIVA, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Carda
authors.affiliation: Centre for Biomaterials and Tissue Engineering, Universitat Politècnica De València, Spain.|Biomedical Research Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Valencia, Spain.
authors.forename: J L Gómez
authors.initials: JL
authors.lastname: Ribelles
authors.affiliation: Centre for Biomaterials and Tissue Engineering, Universitat Politècnica De València, Spain. avalles@ter.upv.es.
authors.forename: A
authors.initials: A
authors.lastname: Vallés-Lluch
mesh_terms: D000497:Alloys; D002448:Cell Adhesion; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D015921:Dental Implants; D003764:Dental Materials / Q000737:chemistry*; D003782:Dental Pulp / Q000166:cytology; D017886:Durapatite / Q000737:chemistry*; D006801:Humans; D016348:Osseointegration; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D000497:Alloys; D020099:Coated Materials, Biocompatible; D015921:Dental Implants; D003764:Dental Materials; C031462:titanium alloy (TiAl6V4); D017886:Durapatite; D014025:Titanium
keywords: dental implantology; hydroxyapatite; osseoinduction; osseointegration; simulated body fluid; titanium alloy