title: Imaging Glycosylation In Vivo by Metabolic Labeling and Magnetic Resonance Imaging.
abstract: Glycosylation is a ubiquitous post-translational modification, present in over 50 % of the proteins in the human genome,1 with important roles in cell-cell communication and migration. Interest in glycome profiling has increased with the realization that glycans can be used as biomarkers of many diseases,2 including cancer.3 We report here the first tomographic imaging of glycosylated tissues in live mice by using metabolic labeling and a gadolinium-based bioorthogonal MRI probe. SignificantN-azidoacetylgalactosamine dependentT1contrast was observed in vivo two hours after probe administration. Tumor, kidney, and liver showed significant contrast, and several other tissues, including the pancreas, spleen, heart, and intestines, showed a very high contrast (>10-fold). This approach has the potential to enable the rapid and non-invasive magnetic resonance imaging of glycosylated tissues in vivo in preclinical models of disease.
authors.affiliation: Cancer Research UK Cambridge Institute Li Ka Shing Centre Cambridge CB2 0RE UK.
authors.forename: André A
authors.identifier: 0000-0003-2740-5166
authors.initials: AA
authors.lastname: Neves
authors.affiliation: Cancer Research UK Cambridge Institute Li Ka Shing Centre Cambridge CB2 0RE UK; Department of Chemistry University of Cambridge Cambridge CB2 1EW UK.
authors.forename: Yéléna A
authors.initials: YA
authors.lastname: Wainman
authors.affiliation: Cancer Research UK Cambridge Institute Li Ka Shing Centre Cambridge CB2 0RE UK.
authors.forename: Alan
authors.initials: A
authors.lastname: Wright
authors.affiliation: Cancer Research UK Cambridge InstituteLi Ka Shing Centre Cambridge CB2 0RE UK; A. I. Virtanen Institute for Molecular Sciences University of Eastern Finland Neulaniementie 2 70211 Kuopio Finland.
authors.forename: Mikko I
authors.initials: MI
authors.lastname: Kettunen
authors.affiliation: Cancer Research UK Cambridge Institute Li Ka Shing Centre Cambridge CB2 0RE UK.
authors.forename: Tiago B
authors.initials: TB
authors.lastname: Rodrigues
authors.affiliation: Cancer Research UK Cambridge Institute Li Ka Shing Centre Cambridge CB2 0RE UK.
authors.forename: Sarah
authors.initials: S
authors.lastname: McGuire
authors.affiliation: Cancer Research UK Cambridge Institute Li Ka Shing Centre Cambridge CB2 0RE UK.
authors.forename: De-En
authors.initials: DE
authors.lastname: Hu
authors.affiliation: Department of Chemistry University of Cambridge Cambridge CB2 1EW UK.
authors.forename: Flaviu
authors.initials: F
authors.lastname: Bulat
authors.affiliation: Department of Molecular Biotechnology and Health Science Molecular Imaging Center Via Nizza 52 10126 Turin Italy.
authors.forename: Simonetta
authors.initials: S
authors.lastname: Geninatti Crich
authors.affiliation: Department of Chemistry University of Cambridge Cambridge CB2 1EW UK.
authors.forename: Henning
authors.initials: H
authors.lastname: Stöckmann
authors.affiliation: Department of Chemistry University of Cambridge Cambridge CB2 1EW UK.
authors.forename: Finian J
authors.identifier: 0000-0003-3408-5199
authors.initials: FJ
authors.lastname: Leeper
authors.affiliation: Cancer Research UK Cambridge Institute Li Ka Shing Centre Cambridge CB2 0RE UK.
authors.forename: Kevin M
authors.identifier: 0000-0003-3883-6287
authors.initials: KM
authors.lastname: Brindle
keywords: Bioorthogonale Chemie; Gadolinium; Glykane; Krebs; Magnetresonanztomographie