title: (1)H NMR studies distinguish the water soluble metabolomic profiles of untransformed and RAS-transformed cells.
abstract: Metabolomic profiling is an increasingly important method for identifying potential biomarkers in cancer cells with a view towards improved diagnosis and treatment. Nuclear magnetic resonance (NMR) provides a potentially noninvasive means to accurately characterize differences in the metabolomic profiles of cells. In this work, we use (1)H NMR to measure the metabolomic profiles of water soluble metabolites extracted from isogenic control and oncogenic HRAS-, KRAS-, and NRAS-transduced BEAS2B lung epithelial cells to determine the robustness of NMR metabolomic profiling in detecting differences between the transformed cells and their untransformed counterparts as well as differences among the RAS-transformed cells. Unique metabolomic signatures between control and RAS-transformed cell lines as well as among the three RAS isoform-transformed lines were found by applying principal component analysis to the NMR data. This study provides a proof of principle demonstration that NMR-based metabolomic profiling can robustly distinguish untransformed and RAS-transformed cells as well as cells transformed with different RAS oncogenic isoforms. Thus, our data may potentially provide new diagnostic signatures for RAS-transformed cells.
authors.affiliation: Department of Chemistry, University of Miami Coral Gables , FL , USA.
authors.forename: Vered
authors.initials: V
authors.lastname: Marks
authors.affiliation: Department of Chemistry, University of MiamiCoral Gables, FL, USA; Department of Medicine, Division of Hematology and Oncology, University of Miami Miller School of Medicine, Miami, FL, USA.
authors.forename: Anisleidys
authors.initials: A
authors.lastname: Munoz
authors.affiliation: Department of Medicine, Division of Hematology and Oncology, University of Miami Miller School of Medicine, Miami, FL, USA; Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL, USA.
authors.forename: Priyamvada
authors.initials: P
authors.lastname: Rai
authors.affiliation: Department of Chemistry, University of Miami Coral Gables , FL , USA.
authors.forename: Jamie D
authors.initials: JD
authors.lastname: Walls
keywords: 1D NOESY; 1H NMR metabolomics; Fieller’s method for unpaired data; Oncogenic RAS