title: Characteristics of aquatic bacterial community and the influencing factors in an urban river.
abstract: Bacteria play a critical role in environmental and ecological processes in river ecosystems. We studied the bacterial community in the Ganjiang River, a major tributary of the Yangtze River, as it flowed through Nanchang, the largest city in the Ganjiang River basin. Water was sampled at five sites monthly during the wet season, and the bacterial community was characterized using Illumina high-throughput sequencing. A total of 811 operational taxonomic units (OTUs) were observed for all samples, ranging from 321 to 519 for each sample. The bacterial communities were maintained by a core of OTUs that persisted longitudinally and monthly. Actinobacteria (41.17% of total sequences) and Proteobacteria (31.80%) were the dominant phyla, while Firmicutes (mostly genus Lactococcus) became most abundant during flooding. Temperature and flow rate, rather than water chemistry, were the main factors influencing the bacterial community in river water. Temperature was the best individual parameter explaining the variations in OTU abundance, while flow rate was the best individual parameter explaining the variations in phylum abundance. Except for Proteobacteria, the relative abundance of bacterial phyla did not differ significantly between sites, and the degrees of influence of urban landscape on the bacterial community were estimated to be 17%-34%.
authors.affiliation: Key Laboratory of Poyang Lake Wetland and Watershed Research, Ministry of Education, Jiangxi Normal University, Nanchang 330022, China; School of Geography and Environment, Jiangxi Normal University, Nanchang 330022, China; Jiangxi Provincial Key Laboratory of Soil Erosion and Prevention, Jiangxi Institute of Soil and Water Conservation, Nanchang 330029, China. Electronic address: wangpengjlu@jxnu.edu.cn.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.affiliation: Key Laboratory of Poyang Lake Wetland and Watershed Research, Ministry of Education, Jiangxi Normal University, Nanchang 330022, China; School of Geography and Environment, Jiangxi Normal University, Nanchang 330022, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Chen
authors.affiliation: School of Environment and Resource, Shanxi University, Taiyuan 030006, China.
authors.forename: Ruiqiang
authors.initials: R
authors.lastname: Yuan
authors.affiliation: Key Laboratory of Poyang Lake Wetland and Watershed Research, Ministry of Education, Jiangxi Normal University, Nanchang 330022, China; School of Geography and Environment, Jiangxi Normal University, Nanchang 330022, China.
authors.forename: Chuangqiong
authors.initials: C
authors.lastname: Li
authors.affiliation: Key Laboratory of Poyang Lake Wetland and Watershed Research, Ministry of Education, Jiangxi Normal University, Nanchang 330022, China; School of Geography and Environment, Jiangxi Normal University, Nanchang 330022, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
mesh_terms: D001419:Bacteria / Q000145:classification*; D002681:China; D002947:Cities; D059014:High-Throughput Nucleotide Sequencing; D064307:Microbiota; D045483:Rivers / Q000382:microbiology*; D012621:Seasons
keywords: Flow rate; Ganjiang river; High-throughput sequencing; Temperature; Urban landscape; Water chemistry