title: Detection and characterization of a novel genotype of porcine astrovirus 4 from nasal swabs from pigs with acute respiratory disease.
abstract: Metagenomic sequencing of three nasal swabs collected from 10- to 21-day-old pigs exhibiting unexplained acute respiratory disease from two different commercial production facilities in Oklahoma identified a novel genotype of porcine astrovirus 4 (PAstV-4). The genomes had only ~75 % nucleotide sequence identity to previously characterized PAstV-4 isolates, while the capsid-encoding ORF2 had only ~53 % amino acid sequence identity to reference strains. A TaqMan assay targeting the novel ORF2 gene found 21 % and 19 % incidence in nasal and fecal swabs, respectively, submitted for unrelated diagnostic testing. PAstV-4 RNA levels were significantly higher (P = 0.04) in nasal swabs, suggesting a possible atypical respiratory tropism.
authors.affiliation: Kansas State Veterinary Diagnostic Laboratory, Department of Diagnostic Medicine/Pathobiology, Kansas State University, 1800 Denison Avenue, Manhattan, Kansas, 66506, USA.
authors.forename: Aiswaria
authors.initials: A
authors.lastname: Padmanabhan
authors.affiliation: Kansas State Veterinary Diagnostic Laboratory, Department of Diagnostic Medicine/Pathobiology, Kansas State University, 1800 Denison Avenue, Manhattan, Kansas, 66506, USA. bhause@vet.k-state.edu.
authors.forename: Ben M
authors.initials: BM
authors.lastname: Hause
mesh_terms: D000208:Acute Disease; D000818:Animals; D019200:Astroviridae / Q000235:genetics / Q000302:isolation & purification*; D019350:Astroviridae Infections / Q000662:veterinary* / Q000821:virology; D015967:Gene Expression Regulation, Viral; D005838:Genotype; D010802:Phylogeny; D012140:Respiratory Tract Diseases / Q000662:veterinary* / Q000821:virology; D013552:Swine; D013553:Swine Diseases / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D014764:Viral Proteins