title: Effect of aqueous and particulate silk fibroin in a rat model of experimental colitis.
abstract: Silk fibroin (SF) has anti-inflammatory properties and promotes wound healing. Moreover, SF particles act as carriers of active drugs against intestinal inflammation due to their capacity to deliver the compound to the damaged colonic tissue. The present work assesses the effect of SF in the trinitrobenzenesulfonic acid model of rat colitis that resembles human intestinal inflammation. SF (8mg/kg) was administered in aqueous solution orally and in two particulate formats by intrarectal route, following two technologies: spray drying to make microparticles and desolvation in organic solvent to produce nanoparticles. SF treatments ameliorated the colonic damage, reduced neutrophil infiltration and improved the compromised oxidative status of the colon. They also reduced the gene expression of pro-inflammatory cytokines like IL-1β and the anti-inflammatory cytokine IL-10. Moreover, they improved the intestinal wall integrity by increasing the gene expression of some of its markers (villin, trefoil factor-3 and mucins), thus accelerating the healing. The immunomodulatory properties of SF particles were also tested in vitro in macrophages: they activated the immune response in basal conditions without increasing it after a pro-inflammatory insult. In conclusion, SF particles could be useful as carriers to deliver active drugs to the damaged intestinal colon with additional anti-inflammatory and healing properties.
authors.affiliation: CIBER-EHD, Department of Pharmacology, ibs.GRANADA, Center for Biomedical Research (CIBM), University of Granada, Avenida del Conocimiento s/n, 18100 Armilla Granada, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Rodriguez-Nogales
authors.affiliation: Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), c/Mayor, 1, 30150 La Alberca, Murcia, Spain.
authors.forename: A A
authors.initials: AA
authors.lastname: Lozano-Pérez
authors.affiliation: Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), c/Mayor, 1, 30150 La Alberca, Murcia, Spain.
authors.forename: S D
authors.initials: SD
authors.lastname: Aznar-Cervantes
authors.affiliation: CIBER-EHD, Department of Pharmacology, ibs.GRANADA, Center for Biomedical Research (CIBM), University of Granada, Avenida del Conocimiento s/n, 18100 Armilla Granada, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Algieri
authors.affiliation: CIBER-EHD, Department of Pharmacology, ibs.GRANADA, Center for Biomedical Research (CIBM), University of Granada, Avenida del Conocimiento s/n, 18100 Armilla Granada, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Garrido-Mesa
authors.affiliation: CIBER-EHD, Department of Pharmacology, ibs.GRANADA, Center for Biomedical Research (CIBM), University of Granada, Avenida del Conocimiento s/n, 18100 Armilla Granada, Spain.
authors.forename: N
authors.initials: N
authors.lastname: Garrido-Mesa
authors.affiliation: CIBER-EHD, Department of Pharmacology, ibs.GRANADA, Center for Biomedical Research (CIBM), University of Granada, Avenida del Conocimiento s/n, 18100 Armilla Granada, Spain.
authors.forename: T
authors.initials: T
authors.lastname: Vezza
authors.affiliation: CIBER-EHD, Department of Pharmacology, ibs.GRANADA, Center for Biomedical Research (CIBM), University of Granada, Avenida del Conocimiento s/n, 18100 Armilla Granada, Spain.
authors.forename: M P
authors.initials: MP
authors.lastname: Utrilla
authors.affiliation: Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), c/Mayor, 1, 30150 La Alberca, Murcia, Spain.
authors.forename: J L
authors.initials: JL
authors.lastname: Cenis
authors.affiliation: CIBER-EHD, Department of Pharmacology, ibs.GRANADA, Center for Biomedical Research (CIBM), University of Granada, Avenida del Conocimiento s/n, 18100 Armilla Granada, Spain.
authors.forename: M E
authors.initials: ME
authors.lastname: Rodríguez-Cabezas
authors.affiliation: CIBER-EHD, Department of Pharmacology, ibs.GRANADA, Center for Biomedical Research (CIBM), University of Granada, Avenida del Conocimiento s/n, 18100 Armilla Granada, Spain. Electronic address: jgalvez@ugr.es.
authors.forename: J
authors.initials: J
authors.lastname: Gálvez
mesh_terms: D000818:Animals; D012831:Bombyx; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D003092:Colitis / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D005349:Fibroins / Q000008:administration & dosage* / Q000737:chemistry; D018836:Inflammation Mediators / Q000037:antagonists & inhibitors / Q000378:metabolism; D051379:Mice; D051381:Rats; D047011:Silk / Q000008:administration & dosage* / Q000737:chemistry; D016896:Treatment Outcome; D014867:Water / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D018836:Inflammation Mediators; D047011:Silk; D014867:Water; D005349:Fibroins
keywords: Drug carrier; Immunomodulation; Microparticles; Nanoparticles; Silk fibroin; TNBS rat colitis