title: Plantamajoside Inhibits UVB and Advanced Glycation End Products-Induced MMP-1 Expression by Suppressing the MAPK and NF-κB Pathways in HaCaT Cells.
abstract: Photoaging and glycation stress are major causes of skin deterioration. Oxidative stress caused by ultraviolet B (UVB) irradiation can upregulate matrix metalloprotease 1 (MMP-1), a major enzyme responsible for collagen damage in the skin. Advanced glycation end products (AGEs) accumulate via gradual formation from skin proteins, especially from long-lived proteins such as dermal elastin and collagen. Plantamajoside (PM), isolated from Plantago asiatica, has various biological effects including anti-inflammatory and antioxidant effects. In this study, we assessed the protective effects of PM on a human keratinocyte cell line (HaCaT) and primary human dermal fibroblasts (HDF) against stress caused by glyceraldehyde-induced AGEs (glycer-AGEs) with UVB irradiation. We found that PM attenuated UVB- and-glycer-AGEs-induced MMP-1 expression in HaCaT and HDF cells and proinflammatory cytokines expression by inhibiting the phosphorylation of mitogen-activated protein kinases (MAPKs) activated by reactive oxygen species. Specific inhibitors of NF-κB and MAPKs attenuated the induced expression of MMP-1. PM also inhibited the phosphorylation of IκBα, and reduced nuclear translocation of NF-κB in these cells. Furthermore, PM attenuated the upregulation of receptor for AGEs (RAGE) by glycer-AGEs with UVB irradiation. Therefore, our findings strongly suggest that PM is a promising inhibitor of skin photoaging.
authors.affiliation: Department of Biotechnology, College of Life Science & Biotechnology, Korea University, Seoul, Korea.
authors.forename: Ah-Ram
authors.initials: AR
authors.lastname: Han
authors.affiliation: Department of Biotechnology, College of Life Science & Biotechnology, Korea University, Seoul, Korea.
authors.forename: Mi-Hyun
authors.initials: MH
authors.lastname: Nam
authors.affiliation: Department of Biotechnology, College of Life Science & Biotechnology, Korea University, Seoul, Korea. kwangwon@korea.ac.kr.
authors.forename: Kwang-Won
authors.initials: KW
authors.lastname: Lee
mesh_terms: D002396:Catechols / Q000494:pharmacology*; D002460:Cell Line; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects* / Q000528:radiation effects; D005960:Glucosides / Q000494:pharmacology*; D017127:Glycation End Products, Advanced / Q000494:pharmacology; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects; D020781:Matrix Metalloproteinase 1 / Q000235:genetics* / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D015595:Skin Aging / Q000187:drug effects / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D002396:Catechols; D005960:Glucosides; D017127:Glycation End Products, Advanced; D016328:NF-kappa B; C421173:plantamajoside; D020928:Mitogen-Activated Protein Kinases; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1