title: Inter-Laboratory Study to Validate New Rapid Screening Methods for Kudoa septempunctata.
abstract: Kudoa septempunctata is the causative agent of a food-borne disease associated with the ingestion of raw olive flounder. As the current qRT-PCR method for its detection is time-consuming, a rapid and simple method is required. Recently, a new real-time loop-mediated isothermal amplification (LAMP) method and an immunochromatography method, whose sensitivities are intended to be compatible with that designated in the official analytical method (10(5) spores/g olive flounder), have been developed. To validate these new methods, we performed an inter-laboratory study across seven laboratories. Both methods could not detect less than 10(4) spores/g; however, these methods were able to detect more than 10(5) spores/g in olive flounder samples. These results demonstrated that the sensitivities of these methods were compatible with the designated level in the official analytical method. We concluded that these new methods were acceptable as the screening methods for K. septempunctata.
authors.affiliation: Division of Microbiology, National Institute of Health Sciences.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Ohnishi
authors.forename: Bongsoo
authors.initials: B
authors.lastname: Lim
authors.forename: Natsumi
authors.initials: N
authors.lastname: Nojima
authors.forename: Ogasawara
authors.initials: O
authors.lastname: Kunitoshi
authors.forename: Shunichi
authors.initials: S
authors.lastname: Inagaki
authors.forename: Koji
authors.initials: K
authors.lastname: Makitsuru
authors.forename: Mari
authors.initials: M
authors.lastname: Sasaki
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Nakane
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Tsuchioka
authors.forename: Kazumi
authors.initials: K
authors.lastname: Horikawa
authors.forename: Maiko
authors.initials: M
authors.lastname: Kawabe
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Minegishi
authors.forename: Nobuo
authors.initials: N
authors.lastname: Miyazaki
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Sugita-Konishi
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods* / Q000592:standards; D005517:Foodborne Diseases / Q000175:diagnosis* / Q000469:parasitology*; D006801:Humans; D055764:Myxozoa / Q000145:classification* / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods* / Q000592:standards; D010272:Parasitic Diseases / Q000175:diagnosis* / Q000469:parasitology*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods / Q000592:standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity