title: Plasmodium falciparum Rab1A Localizes to Rhoptries in Schizonts.
abstract: Over-expression of a GFP-PfRab1A fusion protein in Plasmodium falciparum schizonts produces a punctate pattern of fluorescence typical of rhoptries, secretory organelles involved in host cell invasion. The GFP-positive bodies were purified by a combination of differential and density gradient centrifugation and their protein content determined by MS/MS sequencing. Consistent with the GFP rhoptry-like pattern of transgenic parasites, four of the 19 proteins identified have been previously described to be rhoptry-associated and another four are ER or ER-associated proteins. Confirmation that GFP-PfRab1A decorates rhoptries was obtained by its co-localization with Rap1 and Ron4 in late phase schizonts. We conclude that PfRab1A potentially regulates vesicular traffic from the endoplasmic reticulum to the rhoptries in Apicomplexa parasites.
authors.affiliation: School of BioSciences, University of Melbourne, Melbourne, VIC, 3010, Australia.
authors.forename: David
authors.initials: D
authors.lastname: Morse
authors.affiliation: School of BioSciences, University of Melbourne, Melbourne, VIC, 3010, Australia.
authors.forename: Wesley
authors.initials: W
authors.lastname: Webster
authors.affiliation: School of BioSciences, University of Melbourne, Melbourne, VIC, 3010, Australia.
authors.forename: Ming
authors.initials: M
authors.lastname: Kalanon
authors.affiliation: Laboratoire de Biologie Cellulaire Comparative des Apicomplexes, Institut Cochin, INSERM U1016, CNRS UMR 8104, Université Paris Descartes, 75014, Paris, France.
authors.forename: Gordon
authors.initials: G
authors.lastname: Langsley
authors.affiliation: School of BioSciences, University of Melbourne, Melbourne, VIC, 3010, Australia.
authors.forename: Geoffrey I
authors.initials: GI
authors.lastname: McFadden
mesh_terms: D003001:Cloning, Molecular; D010963:Plasmodium falciparum / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport; D040901:Proteomics / Q000379:methods; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D052939:Schizonts / Q000378:metabolism*; D020761:rab1 GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D015800:Protozoan Proteins; D011993:Recombinant Fusion Proteins; D020761:rab1 GTP-Binding Proteins