title: AFAP-1L1-mediated actin filaments crosslinks hinder Trypanosoma cruzi cell invasion and intracellular multiplication.
abstract: Host actin cytoskeleton polymerization has been shown to play an important role during Trypanosoma cruzi internalization into mammalian cell. The structure and dynamics of the actin cytoskeleton in cells are regulated by a vast number of actin-binding proteins. Here we aimed to verify the impact of AFAP-1L1, during invasion and multiplication of T. cruzi. Knocking-down AFAP-1L1 increased parasite cell invasion and intracellular multiplication. Thus, we have shown that the integrity of the machinery formed by AFAP-1L1 in actin cytoskeleton polymerization is important to hinder parasite infection.
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Brazil.
authors.forename: Karine Canuto Loureiro
authors.initials: KCL
authors.lastname: de Araújo
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Brazil.
authors.forename: Thaise Lara
authors.initials: TL
authors.lastname: Teixeira
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Brazil.
authors.forename: Fabrício Castro
authors.initials: FC
authors.lastname: Machado
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Brazil.
authors.forename: Aline Alves
authors.initials: AA
authors.lastname: da Silva
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Brazil.
authors.forename: Amanda Pifano Neto
authors.initials: APN
authors.lastname: Quintal
authors.affiliation: Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Brazil. Electronic address: silva_cv@yahoo.com.br.
authors.forename: Claudio Vieira
authors.initials: CV
authors.lastname: da Silva
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism*; D048868:Adaptor Proteins, Signal Transducing / Q000494:pharmacology*; D000818:Animals; D014355:Chagas Disease / Q000469:parasitology; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008840:Microfilament Proteins / Q000378:metabolism / Q000494:pharmacology*; D014349:Trypanosoma cruzi / Q000187:drug effects* / Q000378:metabolism
chemical_list: C559217:AFAP1L1 protein, human; D048868:Adaptor Proteins, Signal Transducing; D008840:Microfilament Proteins
keywords: AFAP-1L1; Actin cytoskeleton; Cell invasion; Multiplication; Trypanosoma cruzi