title: Local Effect of Heparin Binding Neurotrophic Factor Combined With Chitosan Entubulization on Sciatic Nerve Repair in Rats.
abstract: OBJECTIVE
  To assess the effect of on sciatic nerve regeneration in animal model of rat.
  
  
  METHODS
  Seventy-five male Wistar rats were divided into five experimental groups randomly (each group containing 15 animals): Sham operation group (SHAM), autograft group (AUTO), transected control (TC), chitosan conduit (CHIT) and heparin binding neurotrophic factor treated group (CHIT/HBNF). In AUTO group a segment of sciatic nerve was transected and reimplanted reversely. In SHAM group sciatic nerve was exposed and manipulated. In transected group left sciatic nerve was transected and stumps were fixed in adjacent muscle (TC). In treatment group defect was bridged using a chitosan conduit (CHIT) filled with 10 µL HBNF (CHIT/HBNF). Each group was subdivided into four subgroups of five animals each and nerve fibers were studied in a 12-week period.
  
  
  RESULTS
  Behavioral, functional, biomechanical, electrophysiological and gastrocnemius muscle mass findings and morphometric indices confirmed faster recovery of regenerated axons in treatment group than in CHIT group (P=0.001). Immunohistochemical reactions to S-100 in treatment group were more positive than that in CHIT group.
  
  
  CONCLUSIONS
  Local administration of improved functional recovery and morphometric indices of sciatic nerve. It could be considered as an effective treatment for peripheral nerve repair in practice.
authors.affiliation: Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Mehrshad
authors.affiliation: Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran.
authors.forename: Ashkan
authors.initials: A
authors.lastname: Seddighnia
authors.affiliation: Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran.
authors.forename: Mohammadreza
authors.initials: M
authors.lastname: Shadabi
authors.affiliation: Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Najafpour
authors.affiliation: Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia 57153 1177, Iran.
authors.forename: Rahim
authors.initials: R
authors.lastname: Mohammadi
keywords: Nerve regeneration; Sciatic; heparin binding neurotrophic factor.