title: [Lycium barbarum polysaccharides enhances SIRT1 expression and decreases MMP-9 and HIF-1α expressions in hypoxic pulmonary vascular smooth muscle cells].
abstract: Objective To investigate the expressions of silent mating type information regulation 2 homolog-1 (SIRT1), matrix metalloproteinase-9 (MMP-9) and hypoxia inducible factor 1α (HIF-1α) in hypoxic pulmonary vascular smooth muscle cells (PVSMCs) treated with Lycium barbarum polysaccharides (LBP). Methods PVSMCs were divided into 10 groups: normal oxygen (200 mL/L oxygen) cells, 2 μmol/mL LBP-treated normal oxygen cells, DMSO-treated normal oxygen cells, DMSO-treated hypoxic (100 mL/L oxygen) cells, (0.5, 1, 2) μmol/mL LBP-treated hypoxic cells; SIRT1 agonist (resveratrol or SRT-1720)-treated hypoxic cells, SIRT1 inhibitor EX-527-treated hypoxic cells. After 6, 12, 24 hours, the mRNA and protein expressions of SIRT1, MMP-9 and HIF-1α were measured by real-time quantitative PCR and Western blotting, respectively. In LBP-treated groups, the expressions of SIRT1, MMP-9 and HIF-1α mRNA and protein were detected 12 hours after LBP treatment. Results Under the condition of hypoxia, the expression levels of SIRT1 mRNA and protein in PVSMCs decreased, while MMP-9 and HIF-1α mRNA increased. Under hypoxia, SIRT1 expression was raised and MMP-9, HIF-1α were reduced by LBP treatment in a dose-dependent manner. Morever, resveratrol could inhibit the expression of MMP-9. Conclusion LBP can enhance the expression of SIRT1 and decrease the expression of MMP-9 and HIF-1α in hypoxic PVSMCs.
authors.affiliation: Department of Clinical Medicine College, Department of Respiratory and Critical Care Medicine, General Hospital, Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Yanni
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Biochemistry and Molecular Biology, Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Yuning
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Clinical Medicine College, Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Wenxia
authors.initials: W
authors.lastname: Guan
authors.affiliation: Department of Biochemistry and Molecular Biology, Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Department of Biochemistry and Molecular Biology, Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry and Molecular Biology, Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Lugen
authors.initials: L
authors.lastname: Bai
authors.affiliation: Department of Clinical Medicine College, Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Kong
authors.affiliation: Department of Respiratory and Critical Care Medicine, General Hospital, Ningxia Medical University, Yinchuan 750004, China. *Corresponding author, E-mail: kansascityfang@hotmail.com.
authors.forename: Fang
authors.initials: F
authors.lastname: Li
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002227:Carbazoles / Q000494:pharmacology; D015687:Cell Hypoxia; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006576:Heterocyclic Compounds, 4 or More Rings / Q000494:pharmacology; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics* / Q000378:metabolism; D008168:Lung / Q000098:blood supply; D020780:Matrix Metalloproteinase 9 / Q000235:genetics* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D032389:Myocytes, Smooth Muscle / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D056564:Sirtuin 1 / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
chemical_list: C550547:6-chloro-2,3,4,9-tetrahydro-1H-carbazole-1-carboxamide; D002227:Carbazoles; D004365:Drugs, Chinese Herbal; D006576:Heterocyclic Compounds, 4 or More Rings; C497444:Hif1a protein, rat; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D012333:RNA, Messenger; C525422:SRT1720; C500223:lycium barbarum polysaccharide; D020780:Matrix Metalloproteinase 9; C488368:Sirt1 protein, rat; D056564:Sirtuin 1