title: Simultaneous determination of six synthetic phenolic antioxidants in edible oils using dispersive liquid-liquid microextraction followed by high-performance liquid chromatography with diode array detection.
abstract: A simple, rapid, organic-solvent- and sample-saving pretreatment technique, called dispersive liquid-liquid microextraction, was developed for the determination of six synthetic phenolic antioxidants from edible oils before high-performance liquid chromatography with diode array detection. The entire procedure was composed of a two-step microextraction and a centrifugal process and could be finished in about 5 min, only consuming only 25 mg of sample and 1 mL of the organic solvent for each extraction. The influences of several important parameters on the microextraction efficiency were thoroughly investigated. Recovery assays for oil samples were spiked at three concentration levels, 50, 100 and 200 mg/kg, and provided recoveries in the 86.3-102.5% range with a relative standard deviation below 3.5%. The intra-day and inter-day precisions for the analysis were less than 3.8%. The proposed method was successfully applied for the determination of synthetic phenolic antioxidants in different oil samples, and satisfactory results were obtained. Thus, the developed method represents a viable alternative for the quality control of synthetic phenolic antioxidant concentrations in edible oils.
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, China.
authors.forename: Shuangjiao
authors.initials: S
authors.lastname: Xu
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, China.
authors.forename: Liangliang
authors.initials: L
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, China.
authors.forename: Yanqin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, China.
authors.forename: Dayun
authors.initials: D
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Kuang
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Fang
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, China.
authors.forename: Shoujun
authors.initials: S
authors.lastname: Wei
authors.affiliation: Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, China.
authors.forename: Aiping
authors.initials: A
authors.lastname: Xiao
authors.affiliation: State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Ma
mesh_terms: D000975:Antioxidants / Q000138:chemical synthesis / Q000737:chemistry* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D057230:Limit of Detection; D059627:Liquid Phase Microextraction / Q000379:methods*; D010636:Phenols / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D010636:Phenols; D010938:Plant Oils
keywords: Dispersive liquid-liquid microextraction; Edible oil analysis; High-performance liquid chromatography; Synthetic phenolic antioxidants