title: Toxic effects and depuration after the dietary lead(II) exposure on the bioaccumulation and hematological parameters in starry flounder (Platichthys stellatus).
abstract: Platichthys stellatus (mean length 20±2cm, mean weight 160.15±15g) were exposed to the different levels of dietary lead(II) at the concentrations of 0, 30, 60, 120, 240mg/kg for 4 weeks. Depuration was conducted for 2 weeks after exposure. The lead exposure over 60mg Pb/kg induced the significant bioaccumulation in tissues of P. stellatus (5-30μg/g tissue), except for brain and muscle where the exposure to 240mg Pb/kg caused the bioaccumulation (2-4μg/g tissue). The hematological parameters such as red blood cell (RBC) counts, hematocrit (Ht) value and hemoglobin (Hb) concentration were substantially decreased over 60mg Pb/kg, and lasted even after the depuration period. For plasma components, calcium and magnesium levels in plasma were generally decreased over 60mg Pb/kg, and glucose level was also mainly increased over 60mg Pb/kg. Total protein was significantly decreased over 120mg Pb/kg after 4 weeks exposure. Glucose and total protein showed the restoration after the depuration period in groups of fish exposed previously to over 60 and 120mg Pb/kg, respectively. However, other parameters that changed during the exposure over 60mg Pb/kg did not recovered. For enzymatic components in plasma, glutamic oxalate transminase (GOT), glutamic pyruvate transminase (GPT) and alkaline phosphatase (ALP) were significantly increased over 120mg Pb/kg, and there was only restoration observed after the depuration for ALP over 120mg Pb/kg.
authors.affiliation: Department of Aquatic Life Medicine, Pukyong National University, Busan 608-737, Republic of Korea.
authors.forename: In-Ki
authors.initials: IK
authors.lastname: Hwang
authors.affiliation: Department of Aquatic Life Medicine, Pukyong National University, Busan 608-737, Republic of Korea.
authors.forename: Kyeong-Wook
authors.initials: KW
authors.lastname: Kim
authors.affiliation: Department of Aquatic Life Medicine, Pukyong National University, Busan 608-737, Republic of Korea. Electronic address: junhwan1982@hanmail.net.
authors.forename: Jun-Hwan
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Aquatic Life Medicine, Pukyong National University, Busan 608-737, Republic of Korea. Electronic address: jckang@pknu.ac.kr.
authors.forename: Ju-Chan
authors.initials: JC
authors.lastname: Kang
mesh_terms: D000818:Animals; D004032:Diet; D004912:Erythrocytes / Q000187:drug effects*; D005432:Flounder / Q000097:blood*; D006400:Hematocrit; D006454:Hemoglobins / Q000032:analysis*; D007854:Lead / Q000097:blood / Q000493:pharmacokinetics / Q000633:toxicity*; D009928:Organ Specificity; D013997:Time Factors; D014018:Tissue Distribution; D014874:Water Pollutants, Chemical / Q000097:blood / Q000493:pharmacokinetics / Q000633:toxicity*
chemical_list: D006454:Hemoglobins; D014874:Water Pollutants, Chemical; D007854:Lead
keywords: Bioaccumulation; Depuration; Dietary lead(II) exposure; Hematological parameters; Platichthys stellatus