title: Identification of SER-PRO-CYS Peptide in Blood Serum of Multiple Sclerosis Patients.
abstract: Monitoring of multiple sclerosis (MS) requires additional molecular markers. Recently, we used original TCA-precipitation/extraction approach in combination with MALDI TOF/TOF mass-spectrometry and identified earlier unknown 48 kDa form of the unconventional myosin IC isoform b (Myo1C) in blood serum of the MS patients. Further examination of TCA-extracted fraction of blood serum of these patients by means of thin-layer chromatography and HPLC gel-filtration allowed detecting 300-500 Da peptides. MALDI TOF/TOF massspectrometry of these peptides showed that they contain Ser-Pro-Cys amino acid sequence. We discussed potential mechanisms of a release of these peptides that were earlier unknown in blood serum of the MS patients.
authors.forename: Severyn
authors.initials: S
authors.lastname: Myronovkij
authors.forename: Nazar
authors.initials: N
authors.lastname: Negrych
authors.forename: Tetyana
authors.initials: T
authors.lastname: Nehrych
authors.forename: Volodymyr
authors.initials: V
authors.lastname: Tkachenko
authors.forename: Serhiy
authors.initials: S
authors.lastname: Souchelnytskyi
authors.forename: Rostyslav
authors.initials: R
authors.lastname: Stoika
authors.affiliation: Institute of Cell Biology, National Academy of Sciences of Ukraine, Drahomanov St., 14/16, 79005, Lviv, Ukraine. kit@cellbiol.lviv.ua.
authors.forename: Yuriy
authors.initials: Y
authors.lastname: Kit
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D015415:Biomarkers / Q000097:blood; D003545:Cysteine / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000378:metabolism*; D010455:Peptides / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification; D011392:Proline / Q000378:metabolism*; D012694:Serine / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D055815:Young Adult
chemical_list: D015415:Biomarkers; D010455:Peptides; D012694:Serine; D011392:Proline; D003545:Cysteine