title: Histomorphometric Evaluation of Bone Formation in Peri-Implant Defects Treated With Different Regeneration Techniques: An Experimental Study in a Rabbit Model.
abstract: OBJECTIVE
  The aim of this study was to investigate the healing of artificially created peri-implant circumferential bone defects using three bone-regeneration techniques.
  
  
  METHODS
  Rabbit tibias (24 rabbits), in which bone defects (9-mm diameter, 4-mm depth) were created and implant beds (3-mm diameter, 6-mm depth) were prepared in the middle of the created defects, were used as the experimental model. Dental implants (3.0 × 10 mm) were inserted, and the peri-implant bone defects were grafted with demineralized freeze-dried bone allograft (DFDBA) plus saline solution, DFDBA plus platelet-rich fibrin (PRF), or DFDBA plus rifamycin. After 4 weeks, the animals were euthanized and the implants with surrounding bone were removed. Undecalcified histomorphometric examinations with toluidine blue staining were performed, and the bone-to-implant contact (BIC) and percentage of new bone formation were evaluated.
  
  
  RESULTS
  The BIC was 50.94% ± 24.39% in the DFDBA-plus-saline solution group, 60.07% ± 4.91% in the DFDBA-plus-rifamycin group, and 73.43% ± 3.86% in the DFDBA-plus-PRF group. The percentage of new bone formation at the defect area was 37.61% ± 1.70% in the DFDBA-plus-saline solution group, 48.51% ± 2.80% in the DFDBA-plus-rifamycin group, and 63.09% ± 2.10% in the DFDBA-plus-PRF group. In terms of new bone formation and BIC, the DFDBA-plus-PRF and DFDBA-plus-rifamycin groups were significantly different from the DFDBA-plus-saline solution group. The difference between the DFDBA-plus-PRF and DFDBA-plus-rifamycin groups was also statistically significant.
  
  
  CONCLUSIONS
  The addition of PRF or rifamycin to DFDBA had a significant positive effect on bone healing in peri-implant bone defects. The DFDBA-plus-PRF group showed the highest percentages of new bone formation and BIC.
authors.affiliation: Resident, Department of Oral and Maxillofacial Surgery, Dental School, Cumhuriyet University, Sivas, Turkey.
authors.forename: Sancar
authors.initials: S
authors.lastname: Şimşek
authors.affiliation: Associate Professor, Department of Oral and Maxillofacial Surgery, Dental School, Cumhuriyet University, Sivas, Turkey. Electronic address: iozec@cumhuriyet.edu.tr.
authors.forename: İlker
authors.initials: İ
authors.lastname: Özeç
authors.affiliation: Professor, Department of Oral and Maxillofacial Surgery, Dental School, Çukurova University, Adana, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Kürkçü
authors.affiliation: Associate Professor, Department of Oral and Maxillofacial Surgery, Dental School, Çukurova University, Adana, Turkey.
authors.forename: Emre
authors.initials: E
authors.lastname: Benlidayı
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D016025:Bone Transplantation / Q000379:methods; D015921:Dental Implants; D048091:Guided Tissue Regeneration / Q000379:methods*; D057893:Immediate Dental Implant Loading; D020007:Implants, Experimental; D008297:Male; D011817:Rabbits; D013194:Staining and Labeling; D013977:Tibia / Q000601:surgery*; D014945:Wound Healing
chemical_list: D015921:Dental Implants