title: Immunohistochemical Analysis of WT1 Antigen Expression in Various Solid Cancer Cells.
abstract: For a peptide-pulsed dendritic cell (DC) vaccine to work effectively in cancer treatment, it is significant that the target protein is expressed in cancer cells. Wilms' tumor 1 (WT1) has been identified as a molecular target for immune cell therapy of cancer. We evaluated the protein expression levels of WT1 in various solid tumors, as well as mucin 1 (MUC1) or major histocompatibility complex (MHC) class l molecules. Seven hundred and thirty-eight patients whose tissue samples were examined by immunohistochemical analysis agreed to undergo DC vaccine therapy. The positive staining of WT1 in tumor cells was observed in 25.3% of patients, with only 8.5% of them showing moderate to strong expression; moreover, WT1 tended to localize in the nucleus and cytoplasm. A positive staining of tumor cells by an anti-MHC class l monoclonal antibody was observed in 98.6% and by an anti-MUC1 monoclonal antibody in 76.8% of the patients. In relation to the application of cancer-specific immunotherapy, these findings provide useful information for determining the efficacy of MUC1- and WT1-targeted therapy.
authors.affiliation: Seta Clinic, Tokyo, Japan naitoh-k@j-immunother.com.
authors.forename: Keiko
authors.initials: K
authors.lastname: Naitoh
authors.affiliation: Seta Clinic, Tokyo, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kamigaki
authors.affiliation: Seta Clinic, Tokyo, Japan.
authors.forename: Eriko
authors.initials: E
authors.lastname: Matsuda
authors.affiliation: Seta Clinic, Tokyo, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ibe
authors.affiliation: Seta Clinic, Tokyo, Japan.
authors.forename: Sachiko
authors.initials: S
authors.lastname: Okada
authors.affiliation: Seta Clinic, Tokyo, Japan.
authors.forename: Eri
authors.initials: E
authors.lastname: Oguma
authors.affiliation: Seta Clinic, Tokyo, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kinoshita
authors.affiliation: Seta Clinic, Tokyo, Japan.
authors.forename: Rishu
authors.initials: R
authors.lastname: Takimoto
authors.affiliation: Seta Clinic, Tokyo, Japan.
authors.forename: Kaori
authors.initials: K
authors.lastname: Makita
authors.affiliation: Seta Clinic, Tokyo, Japan.
authors.forename: Shun
authors.initials: S
authors.lastname: Ogasawara
authors.affiliation: Seta Clinic, Tokyo, Japan.
authors.forename: Shigenori
authors.initials: S
authors.lastname: Goto
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002467:Cell Nucleus / Q000378:metabolism; D002648:Child; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D018396:Mucin-1 / Q000378:metabolism; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology; D025721:WT1 Proteins / Q000378:metabolism*; D055815:Young Adult
chemical_list: D015395:Histocompatibility Antigens Class I; C060192:MUC1 protein, human; D018396:Mucin-1; D025721:WT1 Proteins; C583751:WT1 protein, human
keywords: Immune cell therapy; WT1; dendritic cell vaccine therapy; immunohistochemistry