title: Design and engineering of artificial oxygen-activating metalloenzymes.
abstract: Many efforts are being made in the design and engineering of metalloenzymes with catalytic properties fulfilling the needs of practical applications. Progress in this field has recently been accelerated by advances in computational, molecular and structural biology. This review article focuses on the recent examples of oxygen-activating metalloenzymes, developed through the strategies of de novo design, miniaturization processes and protein redesign. Considerable progress in these diverse design approaches has produced many metal-containing biocatalysts able to adopt the functions of native enzymes or even novel functions beyond those found in Nature.
authors.affiliation: Department of Chemical Sciences, University of Naples "Federico II", Via Cintia, 80126 Naples, Italy. angela.lombardi@unina.it.
authors.forename: Flavia
authors.initials: F
authors.lastname: Nastri
authors.forename: Marco
authors.initials: M
authors.lastname: Chino
authors.forename: Ornella
authors.initials: O
authors.lastname: Maglio
authors.forename: Ambika
authors.initials: A
authors.lastname: Bhagi-Damodaran
authors.forename: Yi
authors.initials: Y
authors.lastname: Lu
authors.forename: Angela
authors.initials: A
authors.lastname: Lombardi
mesh_terms: D004789:Enzyme Activation; D008667:Metalloproteins / Q000737:chemistry* / Q000378:metabolism; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism*; D010105:Oxygenases / Q000737:chemistry / Q000378:metabolism; D015202:Protein Engineering / Q000379:methods*
chemical_list: D008667:Metalloproteins; D010105:Oxygenases; C021555:methane monooxygenase; D010100:Oxygen