title: bFGF Up-regulation Reduces Spontaneous Necrosis of VX2 Tumors Without Increasing Tumoral Microvascular Density.
abstract: OBJECTIVE
  To determine whether up-regulation of basic fibroblast growth factor (bFGF) in VX2 cells reduces tumor necrosis.
  
  
  METHODS
  VX2 cells were transfected with expression vector containing cDNA of rabbit bFGF. Stable clones producing rabbit bFGF (bFGF-VX2) were selected. bFGF-VX2 (n=5) or non-transfected VX2 (control) (n=5) cells were implanted into leg muscle of 10 rabbits. The tumors were characterized 21 days after grafting.
  
  
  RESULTS
  Overexpression of bFGF by VX2 tumors significantly reduced necrosis (p<0.0223) and increased cell viability (p<0.0223), without effect on the mean vascular density. bFGF concentration was significantly higher in bFGF-VX2 tumors (p<0.0062) and negatively correlated with tumor volume at day 21 (ρ=-0.927, p<0.0034). Vascular endothelial growth factor concentration was significantly lower in bFGF-VX2 tumors (p<0.0105) and negatively correlated with the bFGF concentration of tumors (ρ=-0.903, p<0.0067).
  
  
  CONCLUSIONS
  The overexpression of bFGF in VX2 cells increased tumor viability and reduced necrosis, making the evaluation of long-term anticancer therapies possible in this model.
authors.affiliation: ArchimMed, Jouy en Josas, France florentina.pascale@archimmed.com.
authors.forename: Florentina
authors.initials: F
authors.lastname: Pascale
authors.affiliation: ArchimMed, Jouy en Josas, France Department of Pathology, Lariboisière Hospital, Public Assistance & Paris Hospitals, Paris, France.
authors.forename: Saida-Homayra
authors.initials: SH
authors.lastname: Ghegediban
authors.affiliation: ArchimMed, Jouy en Josas, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Bedouet
authors.affiliation: ArchimMed, Jouy en Josas, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Namur
authors.affiliation: Research Center for Interventional Imaging, National Institute of Agronomic Research, Jouy-en-Josas, France/Public Assistance & Paris Hospitals, Paris, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Bonneau
authors.affiliation: ArchimMed, Jouy en Josas, France.
authors.forename: Valentin
authors.initials: V
authors.lastname: Verret
authors.affiliation: Laboratory of Virology and Molecular Immunology, National Institute of Agronomic Research, Jouy en Josas, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Schwartz-Cornil
authors.affiliation: Department of Pathology, Lariboisière Hospital, Public Assistance & Paris Hospitals, Paris, France Faculty of Medicine, Diderot University Paris, Paris, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Wassef
authors.affiliation: Research Center for Interventional Imaging, National Institute of Agronomic Research, Jouy-en-Josas, France/Public Assistance & Paris Hospitals, Paris, France Faculty of Medicine, Diderot University Paris, Paris, France Department of Interventional Neuroradiology, Lariboisière Hospital, Public Assistance & Paris Hospitals, Paris, France.
authors.forename: Alex
authors.initials: A
authors.lastname: Laurent
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental / Q000098:blood supply* / Q000378:metabolism*; D055806:Microvessels / Q000473:pathology; D009336:Necrosis; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000473:pathology; D011817:Rabbits; D015854:Up-Regulation
chemical_list: D016222:Fibroblast Growth Factor 2
keywords: VEGF; VX2; animal models; bFGF; clones; experimental tumors; necrosis; plasmid; rabbit; transfection; vessel density