title: Resonance Raman Probes for Organelle-Specific Labeling in Live Cells.
abstract: Raman microspectroscopy provides for high-resolution non-invasive molecular analysis of biological samples and has a breakthrough potential for dissection of cellular molecular composition at a single organelle level. However, the potential of Raman microspectroscopy can be fully realized only when novel types of molecular probes distinguishable in the Raman spectroscopy modality are developed for labeling of specific cellular domains to guide spectrochemical spatial imaging. Here we report on the design of a next generation Raman probe, based on BlackBerry Quencher 650 compound, which provides unprecedentedly high signal intensity through the Resonance Raman (RR) enhancement mechanism. Remarkably, RR enhancement occurs with low-toxic red light, which is close to maximum transparency in the biological optical window. The utility of proposed RR probes was validated for targeting lysosomes in live cultured cells, which enabled identification and subsequent monitoring of dynamic changes in this organelle by Raman imaging.
authors.affiliation: Institute for Lasers, Photonics and Biophotonics, State University of New York, University at Buffalo, Buffalo, NY 14260, USA.
authors.forename: Andrey N
authors.initials: AN
authors.lastname: Kuzmin
authors.affiliation: Institute for Lasers, Photonics and Biophotonics, State University of New York, University at Buffalo, Buffalo, NY 14260, USA.
authors.forename: Artem
authors.initials: A
authors.lastname: Pliss
authors.affiliation: Institute for Lasers, Photonics and Biophotonics, State University of New York, University at Buffalo, Buffalo, NY 14260, USA.|Center for Theragnosis, Korea Institute of Science and Technology, Seoul 136-791, Republic of Korea.
authors.forename: Chang-Keun
authors.initials: CK
authors.lastname: Lim
authors.affiliation: Institute for Lasers, Photonics and Biophotonics, State University of New York, University at Buffalo, Buffalo, NY 14260, USA.
authors.forename: Jeongyun
authors.initials: J
authors.lastname: Heo
authors.affiliation: Institute for Lasers, Photonics and Biophotonics, State University of New York, University at Buffalo, Buffalo, NY 14260, USA.|Center for Theragnosis, Korea Institute of Science and Technology, Seoul 136-791, Republic of Korea.
authors.forename: Sehoon
authors.initials: S
authors.lastname: Kim
authors.affiliation: Thermo Fisher Scientific, Waltham, MA 02451, USA.
authors.forename: Alexander
authors.initials: A
authors.lastname: Rzhevskii
authors.affiliation: Institute for Lasers, Photonics and Biophotonics, State University of New York, University at Buffalo, Buffalo, NY 14260, USA.|Key Laboratory for Micro-/Nano-Optoelectronic Devices of Ministry of Education, School of Physics and Electronics, Hunan University, Changsha 410082, China.
authors.forename: Bobo
authors.initials: B
authors.lastname: Gu
authors.affiliation: School of Electrical and Electronic Engineering, Nanyang Technological University, 639798, Singapore.
authors.forename: Ken-Tye
authors.initials: KT
authors.lastname: Yong
authors.affiliation: Key Laboratory for Micro-/Nano-Optoelectronic Devices of Ministry of Education, School of Physics and Electronics, Hunan University, Changsha 410082, China.
authors.forename: Shuangchun
authors.initials: S
authors.lastname: Wen
authors.affiliation: Institute for Lasers, Photonics and Biophotonics, State University of New York, University at Buffalo, Buffalo, NY 14260, USA.
authors.forename: Paras N
authors.initials: PN
authors.lastname: Prasad
mesh_terms: D045744:Cell Line, Tumor; D006367:HeLa Cells; D006801:Humans; D008247:Lysosomes / Q000502:physiology; D015335:Molecular Probes / Q000737:chemistry*; D015388:Organelles / Q000502:physiology*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D015335:Molecular Probes