title: Curtain Flow Column: Optimization of Efficiency and Sensitivity.
abstract: Active Flow Technology (AFT) is a form of column technology that increases the separation performance of a HPLC column through the use of a specially purpose built multiport end-fitting(s). Curtain Flow (CF) columns belong to the AFT suite of columns, specifically the CF column is designed so that the sample is injected into the radial central region of the bed and a curtain flow of mobile phase surrounding the injection of solute prevents the radial dispersion of the sample to the wall. The column functions as an 'infinite diameter' column. The purpose of the design is to overcome the radial heterogeneity of the column bed, and at the same time maximize the sample load into the radial central region of the column bed, which serves to increase detection sensitivity. The protocol described herein outlines the system and CF column set up and the tuning process for an optimized infinite diameter 'virtual' column.
authors.affiliation: School of Science and Health, University of Western Sydney.
authors.forename: Sercan
authors.initials: S
authors.lastname: Pravadali-Cekic
authors.affiliation: School of Science and Health, University of Western Sydney.
authors.forename: Danijela
authors.initials: D
authors.lastname: Kocic
authors.affiliation: School of Science and Health, University of Western Sydney.
authors.forename: Stanley
authors.initials: S
authors.lastname: Hua
authors.affiliation: School of Science and Health, University of Western Sydney.
authors.forename: Andrew
authors.initials: A
authors.lastname: Jones
authors.affiliation: School of Science and Health, University of Western Sydney.
authors.forename: Gary
authors.initials: G
authors.lastname: Dennis
authors.affiliation: School of Science and Health, University of Western Sydney; r.shalliker@uws.edu.au.
authors.forename: Andrew
authors.initials: A
authors.lastname: Shalliker
mesh_terms: D002851:Chromatography, High Pressure Liquid