title: Function analysis of Mef2c promoter in muscle differentiation.
abstract: Regeneration of adult skeletal muscle following injury occurs through the activation of satellite cells that proliferates, differentiates, and fuses with injured myofibers. Myocyte enhancer factor 2 (MEF2) proteins are reported to have the potential contributions to adult muscle regeneration. To further understand Mef2c gene, the promoter of pig Mef2c gene was analyzed in this paper. Quantitative real-time PCR (qRT-PCR) revealed the expression pattern of Mef2c gene in muscle of eight tissues. The Mef2c promoter had the higher transcriptional activity in differentiated C2C12 cells than that in proliferating C2C12 cells, which was accompanied by the upregulation of mRNA expression of Mef2c gene. Function deletion and mutation analyses showed that MyoD and MEF2 binding sites within the Mef2c promoter were responsible for the regulation of Mef2c transcription. MEF2C could upregulate the transcriptional activities of Mef2c promoter constructs, which contained a 3'-end nucleotide sequence with p300 binding site. The electrophoretic mobility shift assays and chromatin immunoprecipitation assays determined the MyoD binding site in Mef2c promoter. These results advanced our knowledge of the promoter of the pig Mef2c gene, and the study of Mef2c promoter regulator elements helped to elucidate the regulation mechanisms of Mef2c in muscle differentiation or muscle repair and regeneration.
authors.affiliation: Agricultural Ministry Key Laboratory of Swine Breeding and Genetics & Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Jin
authors.affiliation: Department of Anatomy, Histology and Embryology, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Peng
authors.affiliation: Agricultural Ministry Key Laboratory of Swine Breeding and Genetics & Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China.|Key Projects in the Cooperative Innovation Center for Sustainable Pig Production of Wuhan, Wuhan, Hubei, People's Republic of China.
authors.forename: Siwen
authors.initials: S
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001665:Binding Sites / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics*; D002460:Cell Line; D064326:MEF2 Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D018482:Muscle, Skeletal / Q000502:physiology*; D017570:MyoD Protein / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012038:Regeneration / Q000235:genetics; D013552:Swine; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D064326:MEF2 Transcription Factors; D017570:MyoD Protein
keywords: Pig Mef2c gene; promoter analysis; transcriptional regulation