title: Developing a Biocascade Process: Concurrent Ketone Reduction-Nitrile Hydrolysis of 2-Oxocycloalkanecarbonitriles.
abstract: A stereoselective bioreduction of 2-oxocycloalkanecarbonitriles was concurrently coupled to a whole cell-catalyzed nitrile hydrolysis in one-pot. The first step, mediated by ketoreductases, involved a dynamic reductive kinetic resolution, which led to 2-hydroxycycloalkanenitriles in very high enantio- and diastereomeric ratios. Then, the simultaneous exposure to nitrile hydratase and amidase from whole cells of Rhodococcus rhodochrous provided the corresponding 2-hydroxycycloalkanecarboxylic acids with excellent overall yield and optical purity for the all-enzymatic cascade.
authors.affiliation: Departament of Organic and Inorganic Chemistry, University of Oviedo , Julián Clavería, 8, 33006 Oviedo, Spain.
authors.forename: Elisa
authors.initials: E
authors.lastname: Liardo
authors.affiliation: EntreChem, SL, Edificio Científico Tecnológico , Campus El Cristo, E-33006 Oviedo, Spain.
authors.forename: Nicolás
authors.initials: N
authors.lastname: Ríos-Lombardía
authors.affiliation: EntreChem, SL, Edificio Científico Tecnológico , Campus El Cristo, E-33006 Oviedo, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Morís
authors.affiliation: EntreChem, SL, Edificio Científico Tecnológico , Campus El Cristo, E-33006 Oviedo, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: González-Sabín
authors.affiliation: Departament of Organic and Inorganic Chemistry, University of Oviedo , Julián Clavería, 8, 33006 Oviedo, Spain.
authors.forename: Francisca
authors.initials: F
authors.lastname: Rebolledo