title: Hyaluronan scaffold supports osteogenic differentiation of bone marrow concentrate cells.
abstract: Osteochondral lesions are considered a challenge for orthopedic surgeons. Currently, the treatments available are often unsatisfactory and unable to stimulate tissue regeneration. Tissue engineering offers a new therapeutic strategy, taking into account the role exerted by cells, biomaterial and growth factors in restoring tissue damage. In this light, Mesenchymal Stem Cells (MSCs) have been indicated as a fascinating tool for regenerative medicine thanks to their ability to differentiate into bone, cartilage and adipose tissue. However, in vitro-cultivation of MSCs could be associated with some risks such as de-differentiation/reprogramming, infection and contaminations of the cells. To overcome these shortcomings, a new approach is represented by the use of Bone Marrow Concentrate (BMC), that could allow the delivery of cells surrounded by their microenvironment in injured tissue. For this purpose, cells require a tridimensional scaffold that can support their adhesion, proliferation and differentiation. This study is focused on the potentiality of BMC seeded onto a hyaluronan-based scaffold (Hyaff-11) to differentiate into osteogenic lineage. This process depends on the specific interaction between cells derived from bone marrow (surrounded by their niche) and scaffold, that create an environment able to support the regeneration of damaged tissue. The data obtained from the present study demonstrate that BMC grown onto Hyaff-11 are able to differentiate toward osteogenic sense, producing specific osteogenic genes and matrix proteins.
authors.affiliation: SSD Laboratory RAMSES Rizzoli Orthopedic Institute, Bologna, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Cavallo
authors.affiliation: SSD Laboratory RAMSES Rizzoli Orthopedic Institute, Bologna, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Desando
authors.affiliation: Laboratory of Musculoskeletal Cell Biology, Rizzoli Orthopedic Institute, Bologna, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Ferrari
authors.affiliation: IGM- CNR c/o Rizzoli Orthopedic Institute, Bologna, Italy.
authors.forename: N
authors.initials: N
authors.lastname: Zini
authors.affiliation: SSD Laboratory RAMSES Rizzoli Orthopedic Institute, Bologna, Italy; Department of Medical and Surgical Sciences, University of Bologna, Bologna.
authors.forename: E
authors.initials: E
authors.lastname: Mariani
authors.affiliation: SSD Laboratory RAMSES Rizzoli Orthopedic Institute, Bologna, Italy; SC Laboratory of Immunorheumatology and Tissue Regeneration, Rizzoli Orthopaedic Institute, Bologna, Italy.
authors.forename: B
authors.initials: B
authors.lastname: Grigolo
mesh_terms: D000328:Adult; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation / Q000187:drug effects; D024042:Collagen Type I / Q000032:analysis; D005260:Female; D006801:Humans; D006820:Hyaluronic Acid / Q000494:pharmacology*; D007150:Immunohistochemistry; D008297:Male; D010012:Osteogenesis / Q000187:drug effects*; D012333:RNA, Messenger / Q000032:analysis; D054457:Tissue Scaffolds
chemical_list: D024042:Collagen Type I; D012333:RNA, Messenger; D006820:Hyaluronic Acid