title: Fabrication, characterization and determination of biological activities of poly(ε-caprolactone)/chitosan-caffeic acid composite fibrous mat for wound dressing application.
abstract: In the present study, we designed and fabricated composite fibrous mats consisting of poly(ε-caprolactone) (PCL), chitosan (CH), or chitosan-caffeic acid conjugate (CCA) by an electrospinning technique for wound dressing application. The average fiber diameters of PCL, PCL/CH, and PCL/CCA composite fibrous mats were 1.30±1.07, 1.20±1.22, and 0.94±0.68μm, respectively. Based on universal tensile machine (UTM) analysis, the PCL/CCA fibrous mat significantly increased tensile properties compared with the PCL and PCL/CH fibrous mat. Additionally, initial cell attachment and cell proliferation of the composites using normal human dermal fibrolast-neonatal (NHDF-neo) cells, as well as the antimicrobial effect against Staphylococcus aureus, was investigated. The PCL/CCA fibrous mat show significantly higher initial cell attachment and cell proliferation than the PCL and PCL/CH fibrous mat, and a high antimicrobial effect was observed compared to the PCL and PCL/CH fibrous mat. Based on these results, the PCL/CCA fibrous mat can be used as candidate biomaterial for wound dressing applications and skin tissue engineering.
authors.affiliation: Department of Biomedical Engineering, and Center for Marine-Integrated Biomedical Technology (BK21 Plus), Pukyong National University, Busan 48513, Republic of Korea; Marine-Integrated Bionics Research Center, Pukyong National University, Busan 48513, Republic of Korea.
authors.forename: Gun-Woo
authors.initials: GW
authors.lastname: Oh
authors.affiliation: Department of Biomedical Engineering, and Center for Marine-Integrated Biomedical Technology (BK21 Plus), Pukyong National University, Busan 48513, Republic of Korea; Marine-Integrated Bionics Research Center, Pukyong National University, Busan 48513, Republic of Korea.
authors.forename: Seok-Chun
authors.initials: SC
authors.lastname: Ko
authors.affiliation: Department of Food Science and Technology, Pukyong National University, Busan 48513, Republic of Korea.
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Je
authors.affiliation: Department of Marine-bio Convergence Science, Pukyong National University, Busan 48513, Republic of Korea.
authors.forename: Young-Mog
authors.initials: YM
authors.lastname: Kim
authors.affiliation: Department of Biomedical Engineering, and Center for Marine-Integrated Biomedical Technology (BK21 Plus), Pukyong National University, Busan 48513, Republic of Korea; Marine-Integrated Bionics Research Center, Pukyong National University, Busan 48513, Republic of Korea.
authors.forename: JungHwan
authors.initials: J
authors.lastname: Oh
authors.affiliation: Department of Biomedical Engineering, and Center for Marine-Integrated Biomedical Technology (BK21 Plus), Pukyong National University, Busan 48513, Republic of Korea; Marine-Integrated Bionics Research Center, Pukyong National University, Busan 48513, Republic of Korea. Electronic address: wkjung@pknu.ac.kr.
authors.forename: Won-Kyo
authors.initials: WK
authors.lastname: Jung
mesh_terms: D001458:Bandages; D001672:Biocompatible Materials / Q000737:chemistry*; D002109:Caffeic Acids / Q000737:chemistry* / Q000494:pharmacology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape; D002470:Cell Survival; D048271:Chitosan / Q000737:chemistry*; D055664:Electrochemical Techniques; D006801:Humans; D011091:Polyesters / Q000737:chemistry*; D013499:Surface Properties; D014945:Wound Healing
chemical_list: D001672:Biocompatible Materials; D002109:Caffeic Acids; D011091:Polyesters; C016240:polycaprolactone; D048271:Chitosan
keywords: Chitosan-caffeic acid; Electrospinning; Fibrous mats; Skin tissue engineering; Wound dressing