title: Expression, Purification and Characterization of Recombinant Canine FGF21 in Escherichia coli.
abstract: The canine metabolic diseases, such as obesity and diabetes, have become a worldwide problem. Fibroblast growth factor 21 (FGF21) is a potent regulator which has many biological functions relative to metabolism regulation. It suggests that FGF21 plays important roles in regulating canine metabolic diseases. To acquire the recombinant canine FGF21 (rcFGF21) in Escherichia coli, the recombinant bacteria were induced by 0.5 mM IPTG for 16 hours at 16 °C, and the rcFGF21 protein was purified by Ni-NTA. 8 mg rcFGF21 was acquired from one liter bacteria. The rcFGF21 protein has specific immunoblot reactivity against anti-FGF21 and anti-His antibody. The in vivo experimental result showed that rcFGF21 can significantly reduce plasma glucose of STZ-induced diabetic mice.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Zheng
authors.forename: Chengjun
authors.initials: C
authors.lastname: Yang
authors.forename: Ruofeng
authors.initials: R
authors.lastname: Yin
authors.forename: Jinxi
authors.initials: J
authors.lastname: Jiang
authors.forename: Haiting
authors.initials: H
authors.lastname: He
authors.forename: Xinxin
authors.initials: X
authors.lastname: Wang
authors.forename: Mujie
authors.initials: M
authors.lastname: Kan
authors.affiliation: Department of Biochemistry and Molecular Biology, College of Basic Medical Science, Jilin University, Changchun, 130021, China. yechenxiao2400@126.com.
authors.forename: Yechen
authors.initials: Y
authors.lastname: Xiao
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000032:analysis*; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000378:metabolism; D004283:Dog Diseases / Q000378:metabolism; D004285:Dogs; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D005346:Fibroblast Growth Factors / Q000008:administration & dosage* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D008659:Metabolic Diseases / Q000662:veterinary; D051379:Mice; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D013311:Streptozocin
chemical_list: D001786:Blood Glucose; D011994:Recombinant Proteins; C414620:fibroblast growth factor 21; D007544:Isopropyl Thiogalactoside; D013311:Streptozocin; D005346:Fibroblast Growth Factors