title: Antihypertensive therapy in pre-eclampsia: effects of plasma from nonresponsive patients on endothelial gene expression.
abstract: Over 40% of patients with pre-eclampsia are nonresponsive to antihypertensive therapy, but the underlying mechanisms are unknown. We examined the effects of plasma from nonresponsive and responsive patients on endothelial gene expression.
  
  
  
  PCR array was performed in human umbilical vein endothelial cells (HUVEC) incubated with plasma from nonresponsive (n = 4) and responsive (n = 4) patients. Gene networks and interactions with antihypertensive drugs used in pre-eclampsia were identified by Ingenuity Pathway Analysis.
  
  
  
  Nifedipine and hydralazine act by upregulate or downregulate genes found to be downregulated or upregulated in HUVEC incubated with plasma from nonresponsive patients.
  
  
  
  Our novel findings suggest that plasma from nonresponsive and responsive patients evoke different responses in HUVEC, and might advance the pharmacogenomics research in pre-eclampsia.
authors.affiliation: Department of Pharmacology, Institute of Biosciences of Botucatu, Universidade Estadual Paulista (UNESP), Botucatu, SP 18618-000, Brazil.
authors.forename: Marcelo R
authors.initials: MR
authors.lastname: Luizon
authors.affiliation: Department of Pharmacology, Institute of Biosciences of Botucatu, Universidade Estadual Paulista (UNESP), Botucatu, SP 18618-000, Brazil.
authors.forename: Mayara
authors.initials: M
authors.lastname: Caldeira-Dias
authors.affiliation: Blood Transfusion Center, Cell Engineering Laboratory, Botucatu Medical School, Universidade Estadual Paulista (UNESP), Botucatu, SP 18618-000, Brazil.
authors.forename: Elenice
authors.initials: E
authors.lastname: Deffune
authors.affiliation: Nucleo de Pos-Graduação e Pesquisa, Santa Casa de Belo Horizonte, Belo Horizonte, MG 30150-240, Brazil.
authors.forename: Karla S
authors.initials: KS
authors.lastname: Fernandes
authors.affiliation: Department of Gynecology & Obstetrics, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP 14049-900, Brazil.
authors.forename: Ricardo C
authors.initials: RC
authors.lastname: Cavalli
authors.affiliation: Department of Pharmacology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP 14049-900, Brazil.
authors.forename: José E
authors.initials: JE
authors.lastname: Tanus-Santos
authors.affiliation: Department of Pharmacology, Institute of Biosciences of Botucatu, Universidade Estadual Paulista (UNESP), Botucatu, SP 18618-000, Brazil.
authors.forename: Valéria C
authors.initials: VC
authors.lastname: Sandrim
mesh_terms: D000328:Adult; D000959:Antihypertensive Agents / Q000494:pharmacology / Q000627:therapeutic use; D004730:Endothelium, Vascular / Q000187:drug effects / Q000502:physiology*; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000379:methods*; D053263:Gene Regulatory Networks / Q000187:drug effects / Q000235:genetics*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000502:physiology; D006801:Humans; D010949:Plasma / Q000502:physiology*; D011225:Pre-Eclampsia / Q000175:diagnosis / Q000235:genetics* / Q000628:therapy*; D011247:Pregnancy
chemical_list: D000959:Antihypertensive Agents
keywords: PCR array; antihypertensive drugs; gene expression; human umbilical vein endothelial cells; hydralazine; nifedipine; pathway analysis; pharmacogenomics; pre-eclampsia