title: Different transcriptional levels of GABAAreceptor subunits in mouse cumulus cells around oocytes at different mature stage.
abstract: γ-Aminobutyric acid (GABA) is a principal inhibitory neurotransmitter in the central nervous system (CNS) of vertebrates. However, GABA and its receptor are found not only in peripheral neuronal tissue but also in many peripheral nonneuronal tissues, and thought to have multiple physiological functions. The bidirectional communication between oocytes and cumulus cells (CCs) plays a significant role in oocyte maturation and metabolism. In our previously study, the expression level of α5 subunit in CCs isolated from oocytes of patients with polycystic ovary syndrome had been found to be associated with oocyte nuclear maturity. In this study, we investigated the transcriptional levels of GABAAreceptor subunits in germinal vesicle (GV) and metaphase II (MII) mouse CCs, and explored the role of GABA-A receptor subunits during ovarian follicular development and oocyte maturation. We found that GABAAreceptor subunits exhibited differential transcriptional levels in CCs at different oocyte nuclear maturity stages. It suggested an involvement of GABA-A receptor subunits related to oocyte maturation and certain functions.
authors.affiliation: a Department of Reproductive Medicine , Yantai Yuhuangding Hospital, Affiliated Hospital of Medical College of Qingdao University , Yantai , Shandong , China and.
authors.forename: Huangguan
authors.initials: H
authors.lastname: Dai
authors.affiliation: a Department of Reproductive Medicine , Yantai Yuhuangding Hospital, Affiliated Hospital of Medical College of Qingdao University , Yantai , Shandong , China and.
authors.forename: Cuifang
authors.initials: C
authors.lastname: Hao
authors.affiliation: a Department of Reproductive Medicine , Yantai Yuhuangding Hospital, Affiliated Hospital of Medical College of Qingdao University , Yantai , Shandong , China and.
authors.forename: Xin
authors.initials: X
authors.lastname: Huang
authors.affiliation: a Department of Reproductive Medicine , Yantai Yuhuangding Hospital, Affiliated Hospital of Medical College of Qingdao University , Yantai , Shandong , China and.
authors.forename: Zhenteng
authors.initials: Z
authors.lastname: Liu
authors.affiliation: a Department of Reproductive Medicine , Yantai Yuhuangding Hospital, Affiliated Hospital of Medical College of Qingdao University , Yantai , Shandong , China and.
authors.forename: Huayu
authors.initials: H
authors.lastname: Lian
authors.affiliation: b Medical College of Shandong University, Jinan , Shandong , China.
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
mesh_terms: D000818:Animals; D054885:Cumulus Cells / Q000378:metabolism*; D005260:Female; D051379:Mice; D009865:Oocytes / Q000378:metabolism*; D009866:Oogenesis / Q000502:physiology*; D011963:Receptors, GABA-A / Q000378:metabolism*
chemical_list: D011963:Receptors, GABA-A
keywords: Cumulus cells; GABA; GABA receptor; mouse; oocyte maturation