title: Generation of human embryonic stem cell line expressing green fluorescent protein.
abstract: Human embryonic stem cell line BJNhem20 pCAG-EGFP was generated using non-viral method (Fig. 1). The construct pCAG-EGFP was transfected using microporation procedure.
authors.affiliation: Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India.
authors.forename: Ronak
authors.initials: R
authors.lastname: Shetty
authors.affiliation: Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India.
authors.forename: Deeti
authors.initials: D
authors.lastname: Shetty
authors.affiliation: Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India; Institute for Stem Cell Biology and Regenerative Medicine, Bengaluru, India. Electronic address: inamdar@jncasr.ac.in.
authors.forename: Maneesha S
authors.initials: MS
authors.lastname: Inamdar
mesh_terms: D002454:Cell Differentiation; D002460:Cell Line; D058732:Embryoid Bodies / Q000166:cytology; D005260:Female; D005822:Genetic Vectors / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D000066449:Human Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D059785:Karyotype; D008856:Microscopy, Fluorescence; D016133:Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014162:Transfection
chemical_list: D014157:Transcription Factors; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins