title: Elevated expression of eukaryotic translation initiation factor 3H is associated with proliferation, invasion and tumorigenicity in human hepatocellular carcinoma.
abstract: OBJECTIVE
  We studied the role of eukaryotic translation initiation factor 3 subunit H (EIF3H) in hepatocellular carcinoma (HCC) progression.
  
  
  RESULTS
  High EIF3H expression was observed in 50.23% patients. Upregulation of EIF3H is an independent predictor for greater rates of cancer recurrence and shorter overall survival in HCC patients. Knockdown of EIF3H expression in HCC cells promoted apoptosis, and inhibited cell growth, colony formation, migration, as well as xenograft growth. TGF-βand MAPK pathways are potentially targeted by EIF3H.
  
  
  METHODS
  EIF3H mRNA expression was measured in HCC tissue samples and paired non-tumor samples (N=60) and results were validated in another dataset of 215 HCC patients. Then EIF3H expression and clinical outcomes were correlated. Malignant phenotypes were studied after EIF3H expression was knocked down with siRNA in HCC cell lines. EIF3H targeted pathways were identified by microarray analysis.
  
  
  CONCLUSIONS
  EIF3H is frequently upregulated and is an independent prognostic marker for HCC patients and EIF3H inhibition mitigates the malignant phenotype. Our data provide novel insight into the function of EIF3H in HCC progression, and suggest that EIF3H may be a potentially valuable biomarker for HCC.
authors.affiliation: Department of General Surgery, Jingmen First People's Hospital, Jingmen 448000, Hubei Province, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhu
authors.affiliation: Department of Medical Oncology, Capital Medical University Cancer Center, Beijing Shijitan Hospital, Beijing, 100038, China.
authors.forename: Guo-Liang
authors.initials: GL
authors.lastname: Qiao
authors.affiliation: Department of General Surgery, Jingmen First People's Hospital, Jingmen 448000, Hubei Province, China.
authors.forename: Xiao-Chuan
authors.initials: XC
authors.lastname: Zeng
authors.affiliation: Department of General Surgery, Jingmen First People's Hospital, Jingmen 448000, Hubei Province, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
authors.affiliation: First Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China.
authors.forename: Jian-Jun
authors.initials: JJ
authors.lastname: Yan
authors.affiliation: Department of General Surgery, Jingmen First People's Hospital, Jingmen 448000, Hubei Province, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Duan
authors.affiliation: Department of Hepatobiliary Surgery, Central Hospital of Wuhan, Wuhan 430014, Hubei Province, China.
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Du
mesh_terms: D000818:Animals; D017209:Apoptosis; D063646:Carcinogenesis; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D018450:Disease Progression; D039621:Eukaryotic Initiation Factor-3 / Q000378:metabolism*; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism*; D020935:MAP Kinase Signaling System; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009364:Neoplasm Recurrence, Local; D009368:Neoplasm Transplantation; D011379:Prognosis; D016896:Treatment Outcome
chemical_list: D039621:Eukaryotic Initiation Factor-3
keywords: hepatocellular carcinoma; initiation factors; microarray; prognosis; tumor progression