title: [Role of Treg Cells in Pathogensis of Mouse ITP].
abstract: OBJECTIVE
  To explore the role of Treg cells in the pathogenesis of idiopathic thrombocytopenic purpura (ITP).
  
  
  METHODS
  The ITP mouse model was established, the Treg cell ratio in peripheral blood and spleen was detected by flow cytometry, the CD4+ CD25+ T cells were sorted by immunomagnetic beads, the Treg cell associated transcription factors (Foxp3, Smad7, STAT5 and Akt-1) and cytokines (IL-10, TGF-β) in CD4+ CD25+ T cells were enriched from spleen mononuclear cells, and the mRNA expression of Treg cell was measured by real-time PCR.
  
  
  RESULTS
  The ratio of Tregs in peripheral blood and spleen decreased significantly in ITP mouse, as compared with the controls (P<0.01). In addition, the mRNA expression of IL-10, TGF-β and Foxp3 decreased significantly in spleen CD4+ CD25+ T cells (P<0.05). Expression of Smad 7 mRNA was higher than that of controls.
  
  
  CONCLUSIONS
  The alteration in Treg frequency and function may be responsible for the immune dysfunction in ITP disease. It is also speculated that the lower mRNA expression of Foxp3 and higher mRNA expression of Smad 7 may inhibit the proliferation and differentiation of Treg cells.
authors.affiliation: Department of Hematology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, Guangdong Province, China.|Co-first Authors: ZHANG Ping and LIU Hong-Yun equally contributed to this work.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Department of Hematology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, Guangdong Province, China.|Co-first Authors: ZHANG Ping and LIU Hong-Yun equally contributed to this work.
authors.forename: Hong-Yun
authors.initials: HY
authors.lastname: Liu
authors.affiliation: Department of Hematology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, Guangdong Province, China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Liu
authors.affiliation: Department of Hematology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, Guangdong Province, China.
authors.forename: Shuang-Feng
authors.initials: SF
authors.lastname: Xie
authors.affiliation: Department of Hematology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, Guangdong Province, China.
authors.forename: Xiu-Ju
authors.initials: XJ
authors.lastname: Wang
authors.affiliation: Department of Hematology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, Guangdong Province, China.
authors.forename: Yu-Dan
authors.initials: YD
authors.lastname: Wu
authors.affiliation: Department of Hematology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, Guangdong Province, China.
authors.forename: Guo-Yang
authors.initials: GY
authors.lastname: Zhang
authors.affiliation: Department of Hematology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, Guangdong Province, China.
authors.forename: Peng-Feng
authors.initials: PF
authors.lastname: Yang
authors.affiliation: Department of Hematology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, Guangdong Province, China.
authors.forename: Jian-Xing
authors.initials: JX
authors.lastname: Chang
authors.affiliation: Department of Hematology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, Guangdong Province, China. E-mail: 18922182815@163.com.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Ma
mesh_terms: D000818:Animals; D005434:Flow Cytometry; D051858:Forkhead Transcription Factors / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D051379:Mice; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000276:immunology* / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D051906:Smad7 Protein / Q000378:metabolism; D013154:Spleen / Q000166:cytology; D050378:T-Lymphocytes, Regulatory / Q000166:cytology*; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D012333:RNA, Messenger; D051906:Smad7 Protein; C497807:Smad7 protein, mouse; D016212:Transforming Growth Factor beta; D016753:Interleukin-10