title: Metabolic and behavioral effects of mutant huntingtin deletion in Sim1 neurons in the BACHD mouse model of Huntington's disease.
abstract: Hypothalamic pathology, metabolic dysfunction and psychiatric symptoms are part of Huntington disease (HD), which is caused by an expanded CAG repeat in the huntingtin (HTT) gene. Inactivation of mutant HTT selectively in the hypothalamus prevents the development of metabolic dysfunction and depressive-like behavior in the BACHD mouse model. The hypothalamic paraventricular nucleus (PVN) is implicated in metabolic and emotional control, therefore we here tested whether inactivation of mutant HTT in the PVN affects metabolic and psychiatric manifestations of HD in BACHD mice. BACHD mice were crossed with mice expressing Cre-recombinase under the Sim1 promoter (Sim1-Cre) to inactivate mutant HTT in Sim1 expressing cells, i.e. the PVN of the hypothalamus. We found that inactivation of mutant HTT in Sim1 cells had a sex-specific effect on both the metabolic and the psychiatric phenotype, as these phenotypes were no longer different in male BACHD/Sim1-Cre mice compared to wild-type littermates. We also found a reduced number of GnRH neurons specifically in the anterior hypothalamus and an increased testes weight in male BACHD mice compared to wild-type littermates. Taken together, expression of mutant HTT in Sim1 cells may play a role for the development of metabolic dysfunction and depressive-like behavior in male BACHD mice.
authors.affiliation: Translational Neuroendocrine Research Unit, Department of Experimental Medical Science, Lund University, BMC D11, SE-22184 Lund, Sweden.
authors.forename: Rana
authors.initials: R
authors.lastname: Soylu-Kucharz
authors.affiliation: Translational Neuroendocrine Research Unit, Department of Experimental Medical Science, Lund University, BMC D11, SE-22184 Lund, Sweden.
authors.forename: Barbara
authors.initials: B
authors.lastname: Baldo
authors.affiliation: Translational Neuroendocrine Research Unit, Department of Experimental Medical Science, Lund University, BMC D11, SE-22184 Lund, Sweden.
authors.forename: Åsa
authors.initials: Å
authors.lastname: Petersén
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics*; D001522:Behavior, Animal; D003863:Depression / Q000235:genetics*; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism; D000071058:Huntingtin Protein / Q000235:genetics*; D006816:Huntington Disease / Q000235:genetics / Q000378:metabolism* / Q000523:psychology; D007032:Hypothalamus, Anterior; D008297:Male; D008822:Mice, Transgenic; D009474:Neurons / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics*; D012737:Sex Factors; D013737:Testis
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D007987:Gonadotropin-Releasing Hormone; D000071058:Huntingtin Protein; D012097:Repressor Proteins; C101750:Sim1 protein, mouse