title: In vivo 3D analysis of systemic effects after local heavy-ion beam irradiation in an animal model.
abstract: Radiotherapy is widely used in cancer treatment. In addition to inducing effects in the irradiated area, irradiation may induce effects on tissues close to and distant from the irradiated area. Japanese medaka, Oryzias latipes, is a small teleost fish and a model organism for evaluating the environmental effects of radiation. In this study, we applied low-energy carbon-ion (26.7 MeV/u) irradiation to adult medaka to a depth of approximately 2.2 mm from the body surface using an irradiation system at the National Institutes for Quantum and Radiological Science and Technology. We histologically evaluated the systemic alterations induced by irradiation using serial sections of the whole body, and conducted a heart rate analysis. Tissues from the irradiated side showed signs of serious injury that corresponded with the radiation dose. A 3D reconstruction analysis of the kidney sections showed reductions in the kidney volume and blood cell mass along the irradiated area, reflecting the precise localization of the injuries caused by carbon-beam irradiation. Capillary aneurysms were observed in the gill in both ventrally and dorsally irradiated fish, suggesting systemic irradiation effects. The present study provides an in vivo model for further investigation of the effects of irradiation beyond the locally irradiated area.
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba, Japan.
authors.forename: Kento
authors.initials: K
authors.lastname: Nagata
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba, Japan.
authors.forename: Chika
authors.initials: C
authors.lastname: Hashimoto
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba, Japan.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Watanabe-Asaka
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba, Japan.
authors.forename: Kazusa
authors.initials: K
authors.lastname: Itoh
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Yasuda
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba, Japan.
authors.forename: Kousaku
authors.initials: K
authors.lastname: Ohta
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba, Japan.
authors.forename: Hisako
authors.initials: H
authors.lastname: Oonishi
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba, Japan.
authors.forename: Kento
authors.initials: K
authors.lastname: Igarashi
authors.affiliation: Takasaki Advanced Radiation Research Institute, Quantum Beam Science Research Directorate, National Institutes for Quantum and Radiological Science and Technology, Gunma, Japan.
authors.forename: Michiyo
authors.initials: M
authors.lastname: Suzuki
authors.affiliation: Takasaki Advanced Radiation Research Institute, Quantum Beam Science Research Directorate, National Institutes for Quantum and Radiological Science and Technology, Gunma, Japan.
authors.forename: Tomoo
authors.initials: T
authors.lastname: Funayama
authors.affiliation: Takasaki Advanced Radiation Research Institute, Quantum Beam Science Research Directorate, National Institutes for Quantum and Radiological Science and Technology, Gunma, Japan.
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Kobayashi
authors.affiliation: Department of Anatomy, Kitasato University School of Medicine, Kanagawa, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Nishimaki
authors.affiliation: Department of Anatomy, Kitasato University School of Medicine, Kanagawa, Japan.
authors.forename: Takafumi
authors.initials: T
authors.lastname: Katsumura
authors.affiliation: Department of Anatomy, Kitasato University School of Medicine, Kanagawa, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Oota
authors.affiliation: Department of Anatomy, Kitasato University School of Medicine, Kanagawa, Japan.
authors.forename: Motoyuki
authors.initials: M
authors.lastname: Ogawa
authors.affiliation: Department of Molecular Pathology, Yamaguchi University Graduate School of Medicine, Yamaguchi, Japan.
authors.forename: Atsunori
authors.initials: A
authors.lastname: Oga
authors.affiliation: Department of Molecular Pathology, Yamaguchi University Graduate School of Medicine, Yamaguchi, Japan.
authors.forename: Kenzo
authors.initials: K
authors.lastname: Ikemoto
authors.affiliation: Department of Molecular Pathology, Yamaguchi University Graduate School of Medicine, Yamaguchi, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Itoh
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba, Japan.|LPixel Inc., Tokyo, Japan.
authors.forename: Natsumaro
authors.initials: N
authors.lastname: Kutsuna
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba, Japan.
authors.forename: Shoji
authors.initials: S
authors.lastname: Oda
authors.affiliation: Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mitani
mesh_terms: D000818:Animals; D063193:Heavy Ion Radiotherapy / Q000009:adverse effects*; D007668:Kidney / Q000378:metabolism / Q000473:pathology*; D009206:Myocardium / Q000378:metabolism / Q000473:pathology*; D009990:Oryzias / Q000378:metabolism*; D011833:Radiation Injuries, Experimental / Q000378:metabolism / Q000473:pathology*