title: MiR-10b decreases sensitivity of glioblastoma cells to radiation by targeting AKT.
abstract: BACKGROUND
  Glioblastomas are the most aggressive brain tumors with extremely poor prognosis despite advances in treatment techniques. MiR-10b is highly expressed in glioblastoma and regulates cell proliferation, migration and invasion. Here, we examined the role of MiR-10b on radiotherapy of glioblastomas.
  
  
  METHODS
  MiR-10b mimic or anti-MiR-10b inhibitor was transfected in glioblastoma cells. WST-1 assay was used to examine the effect of MiR-10b on proliferation of transfected glioblastoma cells after radiation treatment. Apoptosis was examined by caspase 3/7 activity and TUNEL assay. The western blot was used to evaluate protein expression.
  
  
  RESULTS
  Altered expression of MiR-10b changed the radiation-induced inhibitory effect on proliferation of glioblastoma cells with dose-dependent manner. MiR-10b decreased radiation-induced apoptosis in glioblastoma cells by activation of caspase 3/7 and inhibition Bcl-2 expression. MiR-10b enhances migration and invasion of glioblastoma cells in presence of radiation. In addition, MiR-10b decreased the sensitivity of glioblastoma cells to radiotherapy by activation of p-AKT expression.
  
  
  CONCLUSIONS
  MiR-10b might be a potential biomarker to predict radiotherapy response and prognosis in glioblastomas.
authors.affiliation: Department of Neurosurgery, Taian Central Hospital, No. 29, Longtan Road, Taian City, Shandong Province China.
authors.forename: Limin
authors.initials: L
authors.lastname: Zhen
authors.affiliation: Department of Neurosurgery, Taian Central Hospital, No. 29, Longtan Road, Taian City, Shandong Province China.
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Taian Central Hospital, No. 29, Longtan Road, Taian City, Shandong Province China.
authors.forename: Mingran
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, Taian Central Hospital, No. 29, Longtan Road, Taian City, Shandong Province China.
authors.forename: Kun
authors.initials: K
authors.lastname: Yang
keywords: AKT; Apoptosis; Glioblastomas; MiR-10b