title: Proteomic dataset for altered glycoprotein expression upon GALNT3 knockdown in ovarian cancer cells.
abstract: This article contains raw and processed data related to research published in "Role of the polypeptide N-acetylgalactosaminyltransferase 3 in ovarian cancer progression: possible implications in abnormal mucin O-glycosylation" [1]. The data presented here was obtained with the application of a bioorthogonal chemical reporter strategy analyzing differential glycoprotein expression following the knock-down (KD) of the GALNT3 gene in the epithelial ovarian cancer (EOC) cell line A2780s. LC-MS/MS mass spectrometry analysis was then performed and the processed data related to the identified glycoproteins show that several hundred proteins are differentially expressed between control and GALNT3 KD A2780s cells. The obtained data also uncover numerous novel glycoproteins; some of which could represent new potential EOC biomarkers and/or therapeutic targets.
authors.affiliation: Department of Molecular Medicine, Laval University, Québec PQ, Canada; Centre de recherche du CHU de Québec, L'Hôtel-Dieu de Québec, Québec PQ, Canada.
authors.forename: Razan
authors.initials: R
authors.lastname: Sheta
authors.affiliation: Centre de recherche du CHU de Québec, CHUL, Québec PQ, Canada.
authors.forename: Florence
authors.initials: F
authors.lastname: Roux-Dalvai
authors.affiliation: Department of Chemistry, Stanford University, Stanford, CA, USA.
authors.forename: Christina M
authors.initials: CM
authors.lastname: Woo
authors.affiliation: Centre de recherche du CHU de Québec, CHUL, Québec PQ, Canada.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Fournier
authors.affiliation: Centre de recherche du CHU de Québec, CHUL, Québec PQ, Canada.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Bourassa
authors.affiliation: Department of Chemistry, Stanford University, Stanford, CA, USA; Howard Hughes Medical Institute, Stanford University, Stanford, CA, USA.
authors.forename: Carolyn R
authors.initials: CR
authors.lastname: Bertozzi
authors.affiliation: Department of Molecular Medicine, Laval University, Québec PQ, Canada; Centre de recherche du CHU de Québec, CHUL, Québec PQ, Canada.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Droit
authors.affiliation: Department of Molecular Medicine, Laval University, Québec PQ, Canada; Centre de recherche du CHU de Québec, L'Hôtel-Dieu de Québec, Québec PQ, Canada.
authors.forename: Dimcho
authors.initials: D
authors.lastname: Bachvarov
keywords: Ac4GalNAz labeling; GALNT3; Glycoproteomics; Glycosylation; Label free quantification; NetOGlyc and NetNGlyc prediction analysis