title: The Transcriptome Study of Subtype M2 Acute Myeloblastic Leukemia.
abstract: Our objective is to explore the tumor-specific mutated genes by transcriptome sequencing of patients with acute myeloblastic leukemia. 96 patients with subtype M2 acute myeloid leukemia (AML), admitted during January 2007 to January 2012, were selected. Bone marrow and peripheral blood samples from the patients after the first visit and the patients who were improved or alleviated, were subjected to high-throughput sequencing to compare the gene expression. The single nucleotide mutation related to subtype M2 AML was detected. Meanwhile, real-time fluorescent quantitation RT-PCR was used to detect the AML1/ETO fusion gene and its correlation with prognosis after treatment. Among 96 patients, AML1-ETO fusion gene was positive in 52 cases, the positive rate was 54.17 %. The complete relief (CR) rate of AML1-ETO fusion gene positive patients was 84.62 %, and the CR rate of AML1/ETO fusion gene negative patients was 77.27 %; the CR rate of AML1-ETO positive patients was higher than that of patients without the fusion gene, however there was no statistical difference. In the analysis of recurrent gene mutation in AML-M2 patients, IDH2, ASXL1, TET2, JAK1 and JAK2 gene expressions were not significantly different before treatment and after CR, however, IDHI, JAK3, ABL1 and BCR gene expressions were significantly different. In the study of transcriptome in AML-M2 patients, high-throughput sequencing could effectively detect the difference of the gene expression before treatment and after CR. Furthermore, positive expression of AML1-ETO fusion gene had effect on the prognosis of patients.
authors.affiliation: Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou, 363000, Fujian, People's Republic of China.
authors.forename: A-Yang
authors.initials: AY
authors.lastname: Wu
authors.affiliation: Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou, 363000, Fujian, People's Republic of China.
authors.forename: Hui-Cong
authors.initials: HC
authors.lastname: Yang
authors.affiliation: Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou, 363000, Fujian, People's Republic of China.
authors.forename: Cong-Meng
authors.initials: CM
authors.lastname: Lin
authors.affiliation: Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou, 363000, Fujian, People's Republic of China.
authors.forename: Bi-de
authors.initials: BD
authors.lastname: Wu
authors.affiliation: Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou, 363000, Fujian, People's Republic of China.
authors.forename: Qi-Shui
authors.initials: QS
authors.lastname: Qu
authors.affiliation: Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou, 363000, Fujian, People's Republic of China.
authors.forename: Yuan-Hai
authors.initials: YH
authors.lastname: Zheng
authors.affiliation: Xiamen University, Xiamen, Fujian, People's Republic of China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wei
authors.affiliation: Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou, 363000, Fujian, People's Republic of China.
authors.forename: Xu-Qiao
authors.initials: XQ
authors.lastname: Mei
authors.affiliation: Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou, 363000, Fujian, People's Republic of China.
authors.forename: Zhen-Hua
authors.initials: ZH
authors.lastname: Zeng
authors.affiliation: Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou, 363000, Fujian, People's Republic of China. drmaxudong@163.com.
authors.forename: Xu-Dong
authors.initials: XD
authors.lastname: Ma
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D050676:Core Binding Factor Alpha 2 Subunit / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D049308:Dioxygenases; D005260:Female; D016044:Fusion Proteins, bcr-abl / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007521:Isocitrate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D053613:Janus Kinase 1 / Q000235:genetics / Q000378:metabolism; D053614:Janus Kinase 2 / Q000235:genetics / Q000378:metabolism; D053612:Janus Kinases / Q000235:genetics / Q000378:metabolism; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D015514:Oncogene Proteins, Fusion / Q000235:genetics / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D000075142:RUNX1 Translocation Partner 1 Protein; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome
chemical_list: C107844:AML1-ETO fusion protein, human; C473949:ASXL1 protein, human; C581634:BCR-ABL1 fusion protein, human; D014408:Biomarkers, Tumor; D050676:Core Binding Factor Alpha 2 Subunit; D004268:DNA-Binding Proteins; D015514:Oncogene Proteins, Fusion; D011518:Proto-Oncogene Proteins; D000075142:RUNX1 Translocation Partner 1 Protein; D012097:Repressor Proteins; C538784:IDH2 protein, human; D007521:Isocitrate Dehydrogenase; D049308:Dioxygenases; C541081:TET2 protein, human; D016044:Fusion Proteins, bcr-abl; C507904:JAK1 protein, human; C507924:JAK2 protein, human; D053613:Janus Kinase 1; D053614:Janus Kinase 2; D053612:Janus Kinases
keywords: AML1/ETO fusion gene; Acute myeloid leukemia; High-throughput; Subtype M2; Transcriptome sequencing