title: Online fast Biospeckle monitoring of drug action in Trypanosoma cruzi parasites by motion history image.
abstract: This paper reports on the application of the motion history image (MHI) method on dynamic laser speckle processing as a result of a specific drug action on Trypanosoma cruzi parasites. The MHI procedure is based on human action recognition, and unlike other methods which use a sequence consisting of several frames for recognition, this method uses only an MHI per action sequence for recognition. MHI method avoids the complexity as well as the large computation in sequence matching-based methods and detects a change in the speckle pattern. Experimental results of MHI on real-time monitoring of activity (motility) under the influence of the drug demonstrate the effectiveness of the proposed method. The MHI showed an online result without loss of resolution and definition if we compare with routine LASCA method. The obtained results highlight the advantage of the MHI analysis over traditional qualitative image intensity-based methods and demonstrate the potential of measuring the activity of parasites via dynamic laser speckle analysis. The data was further numerically analyzed in the time domain, and the results presented the ability of the technique to monitor the action of the drug, particularly Epirubicin (100 μg/ml).
authors.affiliation: Biomedical Optics Laboratory, Department of Applied Physics, Indian School of Mines, Dhanbad, 826004, Jharkhand, India. mohamedzaheer1@gmail.com.
authors.forename: Mohammad Zaheer
authors.identifier: http://orcid.org/0000-0002-0112-238X
authors.initials: MZ
authors.lastname: Ansari
authors.affiliation: Facultad de Farmacia y Bioanálisis, Universidad de los Andes, Mérida, 5101, Venezuela.
authors.forename: Hilda C
authors.initials: HC
authors.lastname: Grassi
authors.affiliation: Centro Multidisciplinario de Ciencias, Instituto Venezolano de Investigaciones Científicas, Mérida, 5101, Venezuela. hcabrera@ictp.it.|SPIE-ICTP Anchor Research in Optics Program Laboratory, International Centre for Theoretical Physics (ICTP), Strada Costiera 11, Trieste, 34151, Italy. hcabrera@ictp.it.
authors.forename: Humberto
authors.initials: H
authors.lastname: Cabrera
authors.affiliation: Centro Multidisciplinario de Ciencias, Instituto Venezolano de Investigaciones Científicas, Mérida, 5101, Venezuela.
authors.forename: Ana
authors.initials: A
authors.lastname: Velásquez
authors.affiliation: Facultad de Farmacia y Bioanálisis, Universidad de los Andes, Mérida, 5101, Venezuela.
authors.forename: Efrén D J
authors.initials: ED
authors.lastname: Andrades
mesh_terms: D000818:Animals; D000981:Antiprotozoal Agents / Q000494:pharmacology*; D021621:Imaging, Three-Dimensional / Q000379:methods*; D007834:Lasers; D009038:Motion; D009862:Online Systems; D010271:Parasites / Q000187:drug effects*; D013997:Time Factors; D014349:Trypanosoma cruzi / Q000187:drug effects*
chemical_list: D000981:Antiprotozoal Agents
keywords: Dynamic speckle; Epirubicin; MHI; Parasites