title: Photo-derived transformation from modified chitosan@calcium carbonate nanohybrids to nanosponges.
abstract: Zwitterionic chitosan (ZC)@calcium carbonate (CC) nanoparticles were conveniently obtained and transformed to biocompatible nanosponges by continuous gas-phase photo-derived transformation in a single-pass configuration, and their potential use for biomedical applications was investigated. The mean diameter of the ZC@CC sponges was ~166 nm (~72 nm for CC and, ~171 nm for ZC), and the sponges had a mesoporous structure (i.e., an average pore diameter of ~13 nm). Measurements of the sponge cytotoxicity were performed and only a slight decrease was observed (>78% in cell viability) when compared with pure ZC (>80%). The ZC@CC sponges had a similar transfection ability to lipofectamine (~2.7 × 10(9) RLU mg(-1) protein) at a 50:1 ratio of sponge:DNA weight. Because of a porous structure, the sponges showed remarkably higher transfection efficiencies than pure ZC.
authors.affiliation: School of Mechanical Engineering, Yeungnam University, Gyeongsan 38541, Republic of Korea.
authors.forename: Jeong Hoon
authors.initials: JH
authors.lastname: Byeon
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D002119:Calcium Carbonate / Q000737:chemistry*; D002448:Cell Adhesion; D002470:Cell Survival; D048271:Chitosan / Q000737:chemistry*; D004247:DNA / Q000032:analysis; D006367:HeLa Cells; D006801:Humans; D008027:Light; D008055:Lipids / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D049329:Nanostructures; D016062:Porosity; D012997:Solvents; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D014162:Transfection; D014466:Ultraviolet Rays
chemical_list: D001672:Biocompatible Materials; D008055:Lipids; C086724:Lipofectamine; D012997:Solvents; D004247:DNA; D048271:Chitosan; D002119:Calcium Carbonate