title: Response surface methodology for the synthesis of an Auricularia auriculajudae polysaccharides-CDDP complex.
abstract: A self-assembly method was used to synthesize the Auricularia auriculajudae polysaccharides (AAPs)-cisplatin (cis-dichlorodiammine platinum (II), CDDP) complexes. Response surface methodology (RSM) was used to optimize the synthesis conditions. The effects of four independent factors: pH (X1: 1-9), time (X2: 0.5-3h), temperature (X3: 20-70°C), and amount of CDDP (X4: 2-22mg) on the platinum content in AAPs-CDDP complexes were evaluated. The RSM results showed that the optimal synthesis conditions were: X1: 7.7, X2: 1.83h, X3: 56.93°C, and X4: 6.02mg. Verification tests showed no significant differences between the practical and the predictive values for each response. Under the optimal conditions, platinum content was 47.0mg/g, without significant differences with the predicted value (47.1mg/g). The FT-IR analysis and1H NMR spectra of AAPs-CDDP complexes showed that the AAPs combined with CDDP using the hydroxyl group on the polysaccharide's chains. In addition, the microphotograph of the AAPs and AAPs-CDDP complexes suggested that an AAPs-CDDP complex was formed. The MTT assay in vitro indicated that AAPs-CDDP complexes exhibited significant anti-tumor activity against Hela and LoVo cells.
authors.affiliation: Department of Food Science, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, China.
authors.forename: Junqiang
authors.initials: J
authors.lastname: Qiu
authors.affiliation: Department of Food Science, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Food Science, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, China. Electronic address: wzy219001@163.com.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Wang
authors.affiliation: The Joint constructed Key Lab of North Drug Research and Application by the Ministry of Education and Provincial Department, Heilongjiang University of Chinese Medicine, Harbin, China.
authors.forename: Shumin
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Food Science, Cornell University, Ithaca, NY, USA.
authors.forename: Joe M
authors.initials: JM
authors.lastname: Regenstein
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000737:chemistry* / Q000494:pharmacology; D001487:Basidiomycota / Q000737:chemistry*; D060326:Chemistry Techniques, Synthetic; D002945:Cisplatin / Q000138:chemical synthesis* / Q000737:chemistry* / Q000494:pharmacology; D062610:Fungal Polysaccharides / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008962:Models, Theoretical
chemical_list: D000970:Antineoplastic Agents; D062610:Fungal Polysaccharides; D002945:Cisplatin
keywords: Auricularia auriculajudae polysaccharides; Cisplatin; Response surface methodology