title: Laser-based technique for controlled damage of mesenchymal cell spheroids: a first step in studying reparation in vitro.
abstract: Modern techniques of laser microsurgery of cell spheroids were used to develop a new simple reproducible model for studying repair and regeneration in vitro Nanosecond laser pulses (wavelength 355 nm, frequency 100 Hz, pulse duration 2 ns) were applied to perform a microdissection of the outer and the inner zones of human bone marrow multipotent mesenchymal stromal cells (BM MMSC) spheroids. To achieve effective dissection and preservation of spheroid viability, the energy of laser pulses was optimized and adjusted in the range 7-9 μJ. After microdissection, the edges of the wound surface opened and the angular opening reached a value of more than 180°. The destruction of the initial spheroid structure was observed in the wound area, with surviving cells changing their shape into a round one. Partial restoration of a spheroid form took place in the first six hours. The complete structure restoration accompanying the reparative processes occurred gradually over seven days due to remodelling of surviving cells.
authors.affiliation: FSBSI Institute of General Pathology and Pathophysiology, 8 Baltiyskaya St, Moscow 125315, Russian Federation Faculty of Biology, Lomonosov Moscow State University, 12-1 Leninskie Gory, Moscow 119234, Russian Federation n_kosheleva@mail.ru.
authors.forename: N V
authors.identifier: http://orcid.org/0000-0002-2665-4972
authors.initials: NV
authors.lastname: Kosheleva
authors.affiliation: Joint Institute for High Temperatures of the Russian Academy of Sciences, 13 Bld 2, Izhorskaya St., Moscow 125412, Russian Federation.
authors.forename: I V
authors.initials: IV
authors.lastname: Ilina
authors.affiliation: FSBSI Institute of General Pathology and Pathophysiology, 8 Baltiyskaya St, Moscow 125315, Russian Federation.
authors.forename: I M
authors.initials: IM
authors.lastname: Zurina
authors.affiliation: Faculty of Biology, Lomonosov Moscow State University, 12-1 Leninskie Gory, Moscow 119234, Russian Federation.
authors.forename: A E
authors.initials: AE
authors.lastname: Roskova
authors.affiliation: FSBSI Institute of General Pathology and Pathophysiology, 8 Baltiyskaya St, Moscow 125315, Russian Federation.
authors.forename: A A
authors.initials: AA
authors.lastname: Gorkun
authors.affiliation: Joint Institute for High Temperatures of the Russian Academy of Sciences, 13 Bld 2, Izhorskaya St., Moscow 125412, Russian Federation.
authors.forename: A V
authors.initials: AV
authors.lastname: Ovchinnikov
authors.affiliation: Joint Institute for High Temperatures of the Russian Academy of Sciences, 13 Bld 2, Izhorskaya St., Moscow 125412, Russian Federation.
authors.forename: M B
authors.initials: MB
authors.lastname: Agranat
authors.affiliation: FSBSI Institute of General Pathology and Pathophysiology, 8 Baltiyskaya St, Moscow 125315, Russian Federation Russian Medical Academy of Postgraduate Education, 2/1 Barrikadnaya St., Moscow 123995, Russian Federation.
authors.forename: I N
authors.initials: IN
authors.lastname: Saburina
keywords: Laser microsurgery; Multipotent mesenchymal stromal cells; Nanosecond laser scalpel; Reparation; Spheroids