title: MicroRNA-214 promotes peritoneal metastasis through regulating PTEN negatively in gastric cancer.
abstract: OBJECTIVE
  We aimed to investigate the effects of microRNA-214 (miR-214) on peritoneal metastasis as well as to elucidate its regulatory mechanism in gastric cancer (GC).
  
  
  METHODS
  The expression levels of miR-214 in human GC cell lines MKN-28NM, MKN-28M, GC9811 and GC9811-P were analyzed by quantitative real-time PCR. Lentiviral miR-214, lentiviral miR-214 inhibitor, and empty lentiviral vector were transfected to GC cell lines, respectively. The roles of miR-214 in cell invasion, migration, proliferation and colony-forming ability were then analyzed. Besides, the expression levels of PTEN in different transfected cells were determined by western blot analysis.
  
  
  RESULTS
  We found that miR-214 was up-regulated in GC9811-P cells with high metastatic potential to the peritoneum compared with that in GC9811 cells. In addition, in vitro overexpression of miR-214 promoted cell invasion, migration, proliferation and colony-forming ability of GC9811 cells, while down-regulation of miR-214 had opposite effects in GC9811-P cells. Besides, overexpression of miR-214 in GC9811 cells markedly down-regulated PTEN expression, whereas down-regulation of miR-214 in GC9811-P cells significantly increased PTEN expression.
  
  
  CONCLUSIONS
  Our findings indicate that miR-214 may promote peritoneal metastasis of GC cells via down-regulation of PTEN, thus leading to the progression of GC.
authors.affiliation: Ningxia Hui Autonomous Region People's Hospital, Department of Gastroenterology, Yinchuan, China.
authors.forename: Ruijuan
authors.initials: R
authors.lastname: Xin
authors.affiliation: Ningxia Hui Autonomous Region People's Hospital, Department of Gastroenterology, Yinchuan, China.
authors.forename: Feihu
authors.initials: F
authors.lastname: Bai
authors.affiliation: Ningxia Hui Autonomous Region People's Hospital, Department of Gastroenterology, Yinchuan, China.
authors.forename: Yaning
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Ningxia Medical University, Yinchuan, China.
authors.forename: Mengna
authors.initials: M
authors.lastname: Jiu
authors.affiliation: Ningxia Medical University, Yinchuan, China.
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Liu
authors.affiliation: Affiliated Hospital of Ningxia Medical University, Department of Gastroenterology, Yinchuan, China.
authors.forename: Fangyun
authors.initials: F
authors.lastname: Bai
authors.affiliation: Xijing Hospital of Digestive Diseases, State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Beilin District, Changlexi Road, 710000 Xi'an, China. Electronic address: yongzhannie@yeah.net.
authors.forename: Yongzhan
authors.initials: Y
authors.lastname: Nie
authors.affiliation: Xijing Hospital of Digestive Diseases, State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Beilin District, Changlexi Road, 710000 Xi'an, China.
authors.forename: Daiming
authors.initials: D
authors.lastname: Fan
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D015536:Down-Regulation; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D051059:PTEN Phosphohydrolase / Q000235:genetics*; D010534:Peritoneal Neoplasms / Q000556:secondary*; D060888:Real-Time Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000473:pathology*; D015854:Up-Regulation
chemical_list: C547397:MIRN214 microRNA, human; D035683:MicroRNAs; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human