title: The fission yeast CENP-B protein Abp1 prevents pervasive transcription of repetitive DNA elements.
abstract: It is well established that eukaryotic genomes are pervasively transcribed producing cryptic unstable transcripts (CUTs). However, the mechanisms regulating pervasive transcription are not well understood. Here, we report that the fission yeast CENP-B homolog Abp1 plays an important role in preventing pervasive transcription. We show that loss of abp1 results in the accumulation of CUTs, which are targeted for degradation by the exosome pathway. These CUTs originate from different types of genomic features, but the highest increase corresponds to Tf2 retrotransposons and rDNA repeats, where they map along the entire elements. In the absence of abp1, increased RNAPII-Ser5P occupancy is observed throughout the Tf2 coding region and, unexpectedly, RNAPII-Ser5P is enriched at rDNA repeats. Loss of abp1 also results in Tf2 derepression and increased nucleolus size. Altogether these results suggest that Abp1 prevents pervasive RNAPII transcription of repetitive DNA elements (i.e., Tf2 and rDNA repeats) from internal cryptic sites.
authors.affiliation: Institute of Molecular Biology of Barcelona, CSIC, Baldiri Reixac, 4, 08028 Barcelona, Spain; Institute for Research in Biomedicine, IRB Barcelona, The Barcelona Institute of Science and Technology, Baldiri Reixac, 10, 08028 Barcelona, Spain.
authors.forename: Anne
authors.initials: A
authors.lastname: Daulny
authors.affiliation: Institute of Molecular Biology of Barcelona, CSIC, Baldiri Reixac, 4, 08028 Barcelona, Spain; Institute for Research in Biomedicine, IRB Barcelona, The Barcelona Institute of Science and Technology, Baldiri Reixac, 10, 08028 Barcelona, Spain.
authors.forename: Eva
authors.initials: E
authors.lastname: Mejía-Ramírez
authors.affiliation: Institute for Research in Biomedicine, IRB Barcelona, The Barcelona Institute of Science and Technology, Baldiri Reixac, 10, 08028 Barcelona, Spain.
authors.forename: Oscar
authors.initials: O
authors.lastname: Reina
authors.affiliation: Rutgers, The State University of New Jersey, Piscataway, USA.
authors.forename: Jesus
authors.initials: J
authors.lastname: Rosado-Lugo
authors.affiliation: Institute of Molecular Biology of Barcelona, CSIC, Baldiri Reixac, 4, 08028 Barcelona, Spain; Institute for Research in Biomedicine, IRB Barcelona, The Barcelona Institute of Science and Technology, Baldiri Reixac, 10, 08028 Barcelona, Spain.
authors.forename: Lorena
authors.initials: L
authors.lastname: Aguilar-Arnal
authors.affiliation: Institute for Research in Biomedicine, IRB Barcelona, The Barcelona Institute of Science and Technology, Baldiri Reixac, 10, 08028 Barcelona, Spain.
authors.forename: Herbert
authors.initials: H
authors.lastname: Auer
authors.affiliation: Rutgers, The State University of New Jersey, Piscataway, USA. Electronic address: Zaratiegui@dls.rutgers.edu.
authors.forename: Mikel
authors.initials: M
authors.lastname: Zaratiegui
authors.affiliation: Institute of Molecular Biology of Barcelona, CSIC, Baldiri Reixac, 4, 08028 Barcelona, Spain; Institute for Research in Biomedicine, IRB Barcelona, The Barcelona Institute of Science and Technology, Baldiri Reixac, 10, 08028 Barcelona, Spain. Electronic address: fambmc@ibmb.csic.es.
authors.forename: Fernando
authors.initials: F
authors.lastname: Azorin
mesh_terms: D002466:Cell Nucleolus / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D002503:Centromere / Q000378:metabolism / Q000648:ultrastructure; D051936:Centromere Protein B / Q000235:genetics / Q000378:metabolism; D004275:DNA, Ribosomal / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000172:deficiency / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D006570:Heterochromatin / Q000378:metabolism / Q000648:ultrastructure; D012319:RNA Polymerase II / Q000235:genetics* / Q000378:metabolism; D020871:RNA Stability; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018626:Retroelements; D012568:Schizosaccharomyces / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D051936:Centromere Protein B; D004275:DNA, Ribosomal; D004268:DNA-Binding Proteins; D006570:Heterochromatin; D012333:RNA, Messenger; D018626:Retroelements; D029702:Schizosaccharomyces pombe Proteins; C083145:Tf2-6 protein, S pombe; C097691:cbp1 protein, S pombe; D012319:RNA Polymerase II
keywords: Abp1; CENP-B; CUTs; Chromatin; Cryptic transcription; Epigenetics; Schizosaccharomyces pombe; Tf2; rDNA