title: Nucleolus-like body of mouse oocytes contains lamin A and B and TRF2 but not actin and topo II.
abstract: BACKGROUND
  During the final stages of oocyte development, all chromosomes join in a limited nuclear volume for the final formation of a single complex chromatin structure - the karyosphere. In the majority of mammalian species, the chromosomes surround a round protein/fibrillar body known as the central body, or nucleolus-like body (NLB). Nothing seems to unite the inner portion of the karyosphere with the nucleolus except position at its remnants. Nevertheless, in this study we will use term NLB as the conventional one for karyosphere with the central body. At the morphological level, NLBs consist of tightly-packed fibres of 6-10 nm. The biochemical structure of this dense, compact NLB fibre centre remains uncertain.
  
  
  RESULTS
  The aim of this study was to determine which proteins represent the NLB components at final stages of karyosphere formation in mouse oogenesis. To determine this, three antibodies (ABs) have been examined against different actin epitopes. Examination of both ABs against the actin N-end provided similar results: spots inside the nucleus. Double staining with AB against SC35 and actin revealed the colocalization of these proteins in IGCs (interchromatin granule clusters/nuclear speckles/SC35 domains). In contrast, examination of polyclonal AB against peptide at the C-end reveals a different result: actin is localized exclusively in connection with the chromatin. Surprisingly, no forms of actin or topoisomerase II are present as components of the NLB. It was discovered that: (1) lamin B is an NLB component from the beginning of NLB formation, and a major portion of it resides in the NLB at the end of oocyte development; (2) lamin A undergoes rapid movement into the NLB, and a majority of it remains in the NLB; (3) the telomere-binding protein TRF2 resides in the IGCs/nuclear speckles until the end of oocyte development, when significant part of it transfers to the NLB.
  
  
  CONCLUSIONS
  NLBs do not contain actin or topo II. Lamin B is involved from the beginning of NLB formation. Both Lamin A and TRF2 exhibit rapid movement to the NLB at the end of oogenesis. This dynamic distribution of proteins may reflect the NLB's role in future chromatin organization post-fertilisation.
authors.affiliation: Institute of Cytology, Russian Academy of Sciences, St Petersburg, 194064 Russia.
authors.forename: Galina N
authors.initials: GN
authors.lastname: Pochukalina
authors.affiliation: Institute of Cytology, Russian Academy of Sciences, St Petersburg, 194064 Russia.
authors.forename: Nadya V
authors.initials: NV
authors.lastname: Ilicheva
authors.affiliation: Institute of Cytology, Russian Academy of Sciences, St Petersburg, 194064 Russia ; Saint Petersburg State University, St Petersburg, 199034 Russia ; Far Eastern Federal University, Vladivostok, 690950 Russia.
authors.forename: Olga I
authors.initials: OI
authors.lastname: Podgornaya
authors.affiliation: Institute of Cytology, Russian Academy of Sciences, St Petersburg, 194064 Russia ; Saint Petersburg State University, St Petersburg, 199034 Russia.
authors.forename: Alexey P
authors.initials: AP
authors.lastname: Voronin
keywords: Immunofluorescence; Karyosphere; Morphology; Mouse oogenesis; Nucleolus-like body