title: Ubiquitin-conjugating enzyme UbcH10 promotes gastric cancer growth and is a potential biomarker for gastric cancer.
abstract: Gastric cancer is a fatal disease and the availability of early diagnostic methods is limited. There is an urgent need to identify effective targets for early diagnosis and therapeutics. UbcH10 is a ubiquitin-conjugating enzyme with high expression in various types of cancers. In the present study, several gastric tumor cell lines with high or low expression of UbcH10 were exploited to study the role of UbcH10 in gastric cancer. Knockdown of UbcH10 expression using siRNA in gastric cancer cell lines with high expression of UbcH10 resulted in reduced proliferation, increased cisplatin-induced apoptosis and reduced serum-induced ERK, Akt and p38 phosphorylation signaling. In agreement, overexpression of UbcH10 in gastric cancer cell lines with low expression of UbcH10 led to enhanced cell proliferation and resistance to cisplatin-induced apoptosis. Most importantly, IHC analyses showed that the UbcH10 protein was expressed at a high level in most patient gastric cancer tissues, but was absent in adjacent mesenchyme tissues. These data suggest that UbcH10 may promote gastric cancer growth and can serve as a biomarker for diagnosis or as a target for novel therapeutics in gastric cancer.
authors.affiliation: Minhang Hospital, Fudan University, Shanghai 201199, P.R. China.
authors.forename: Mengxuan
authors.initials: M
authors.lastname: Yang
authors.affiliation: Shanghai ChemPartner Co., Ltd., Shanghai 201203, P.R. China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Qu
authors.affiliation: Minhang Hospital, Fudan University, Shanghai 201199, P.R. China.
authors.forename: Rongliang
authors.initials: R
authors.lastname: Shi
authors.affiliation: Minhang Hospital, Fudan University, Shanghai 201199, P.R. China.
authors.forename: Xubo
authors.initials: X
authors.lastname: Wu
authors.affiliation: Minhang Hospital, Fudan University, Shanghai 201199, P.R. China.
authors.forename: Chang
authors.initials: C
authors.lastname: Su
authors.affiliation: Minhang Hospital, Fudan University, Shanghai 201199, P.R. China.
authors.forename: Zhiqiu
authors.initials: Z
authors.lastname: Hu
authors.affiliation: Minhang Hospital, Fudan University, Shanghai 201199, P.R. China.
authors.forename: Qimeng
authors.initials: Q
authors.lastname: Chang
authors.affiliation: Minhang Hospital, Fudan University, Shanghai 201199, P.R. China.
authors.forename: Shaoqun
authors.initials: S
authors.lastname: Liu
authors.affiliation: Minhang Hospital, Fudan University, Shanghai 201199, P.R. China.
authors.forename: Gaofeng
authors.initials: G
authors.lastname: Pan
authors.affiliation: Minhang Hospital, Fudan University, Shanghai 201199, P.R. China.
authors.forename: Ming
authors.initials: M
authors.lastname: Lei
authors.affiliation: Shanghai ChemPartner Co., Ltd., Shanghai 201203, P.R. China.
authors.forename: Fubo
authors.initials: F
authors.lastname: Xie
authors.affiliation: Shanghai ChemPartner Co., Ltd., Shanghai 201203, P.R. China.
authors.forename: Shiwei
authors.initials: S
authors.lastname: Tu
authors.affiliation: Shanghai ChemPartner Co., Ltd., Shanghai 201203, P.R. China.
authors.forename: Weikang
authors.initials: W
authors.lastname: Tao
authors.affiliation: Shanghai ChemPartner Co., Ltd., Shanghai 201203, P.R. China.
authors.forename: He
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Shanghai ChemPartner Co., Ltd., Shanghai 201203, P.R. China.
authors.forename: Gang
authors.initials: G
authors.lastname: Hu
authors.affiliation: Minhang Hospital, Fudan University, Shanghai 201199, P.R. China.
authors.forename: Ziping
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis / Q000235:genetics; D014408:Biomarkers, Tumor / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D006801:Humans; D020935:MAP Kinase Signaling System / Q000235:genetics; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology*; D044763:Ubiquitin-Conjugating Enzymes / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; D034741:RNA, Small Interfering; C105156:UBE2C protein, human; D044763:Ubiquitin-Conjugating Enzymes; D051057:Proto-Oncogene Proteins c-akt