title: A qPCR aptasensor for sensitive detection of aflatoxin M1.
abstract: Aflatoxin M1 (AFM1), one of the most toxic mycotoxins, imposes serious health hazards. AFM1 had previously been classified as a group 2B carcinogen [1] and has been classified as a group 1 carcinogen by the International Agency for Research on Cancer (IARC) of the World Health Organization (WHO) [2]. Determination of AFM1 thus plays an important role for quality control of food safety. In this work, a sensitive and reliable aptasensor was developed for the detection of AFM1. The immobilization of aptamer through a strong interaction with biotin-streptavidin was used as a molecular recognition element, and its complementary ssDNA was employed as the template for a real-time quantitative polymerase chain reaction (RT-qPCR) amplification. Under optimized assay conditions, a linear relationship (ranging from 1.0 × 10(-4) to 1.0 μg L(-1)) was achieved with a limit of detection (LOD) down to 0.03 ng L(-1). In addition, the aptasensor developed here exhibits high selectivity for AFM1 over other mycotoxins and small effects from cross-reaction with structural analogs. The method proposed here has been successfully applied to quantitative determination of AFM1 in infant rice cereal and infant milk powder samples. Results demonstrated that the current approach is potentially useful for food safety analysis, and it could be extended to a large number of targets.
authors.affiliation: Ministry of Agriculture Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2, Yuan Ming Yuan West Road, Haidian District, Beijing, 100193, China.|Chimie générale et organique, Gembloux Agro-Bio Tech, Université de Liège, Passage des Déportés 2, 5030, Gembloux, Belgium.|Ministry of Agriculture-Milk and Dairy Product Inspection Center (Beijing), Beijing, 100193, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Guo
authors.affiliation: Ministry of Agriculture Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2, Yuan Ming Yuan West Road, Haidian District, Beijing, 100193, China.|Ministry of Agriculture-Milk and Dairy Product Inspection Center (Beijing), Beijing, 100193, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wen
authors.affiliation: Ministry of Agriculture Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2, Yuan Ming Yuan West Road, Haidian District, Beijing, 100193, China.|Ministry of Agriculture-Milk and Dairy Product Inspection Center (Beijing), Beijing, 100193, China.|State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Zheng
authors.affiliation: Ministry of Agriculture Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2, Yuan Ming Yuan West Road, Haidian District, Beijing, 100193, China.|Ministry of Agriculture-Milk and Dairy Product Inspection Center (Beijing), Beijing, 100193, China.
authors.forename: Songli
authors.initials: S
authors.lastname: Li
authors.affiliation: Chimie générale et organique, Gembloux Agro-Bio Tech, Université de Liège, Passage des Déportés 2, 5030, Gembloux, Belgium.
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Fauconnier
authors.affiliation: Ministry of Agriculture Laboratory of Quality & Safety Risk Assessment for Dairy Products (Beijing), Institute of Animal Science, Chinese Academy of Agricultural Sciences, No. 2, Yuan Ming Yuan West Road, Haidian District, Beijing, 100193, China. jiaqiwang@vip.163.com.|Ministry of Agriculture-Milk and Dairy Product Inspection Center (Beijing), Beijing, 100193, China. jiaqiwang@vip.163.com.|State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. jiaqiwang@vip.163.com.
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Wang
mesh_terms: D016607:Aflatoxin M1 / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics; D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000235:genetics; D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D052157:Aptamers, Nucleotide; D016607:Aflatoxin M1
keywords: Aflatoxin M1; Aptamer; Food safety; RT-qPCR