title: Desorption Electrospray Ionization (DESI) Mass Spectrometric Imaging of the Distribution of Rohitukine in the Seedling of Dysoxylum binectariferum Hook. F.
abstract: Ambient ionization mass spectrometric imaging of all parts of the seedling of Dysoxylum binectariferum Hook. f (Meliaceae) was performed to reconstruct the molecular distribution of rohitukine (Rh) and related compounds. The species accumulates Rh, a prominent chromone alkaloid, in its seeds, fruits, and stem bark. Rh possesses anti-inflammatory, anti-cancer, and immuno-modulatory properties. Desorption electrospray ionization mass spectrometry imaging (DESI MSI) and electrospray ionization (ESI) tandem mass spectrometry (MS/MS) analysis detected Rh as well as its glycosylated, acetylated, oxidized, and methoxylated analogues. Rh was predominantly distributed in the main roots, collar region of the stem, and young leaves. In the stem and roots, Rh was primarily restricted to the cortex region. The identities of the metabolites were assigned based on both the fragmentation patterns and exact mass analyses. We discuss these results, with specific reference to the possible pathways of Rh biosynthesis and translocation during seedling development in D. binectariferum.
authors.affiliation: DST Unit of Nanoscience and Thematic Unit of Excellence, Department of Chemistry, Indian Institute of Technology Madras, Chennai, 600036, India.
authors.forename: Patel
authors.initials: P
authors.lastname: Mohana Kumara
authors.affiliation: DST Unit of Nanoscience and Thematic Unit of Excellence, Department of Chemistry, Indian Institute of Technology Madras, Chennai, 600036, India.
authors.forename: Amitava
authors.initials: A
authors.lastname: Srimany
authors.affiliation: DST Unit of Nanoscience and Thematic Unit of Excellence, Department of Chemistry, Indian Institute of Technology Madras, Chennai, 600036, India.
authors.forename: Suganya
authors.initials: S
authors.lastname: Arunan
authors.affiliation: School of Ecology and Conservation, Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bengaluru, 560065, India.|Ashoka Trust for Research in Ecology and the Environment, Royal Enclave, Sriramapura, Jakkur, Bengaluru, 560064, India.
authors.forename: Gudasalamani
authors.initials: G
authors.lastname: Ravikanth
authors.affiliation: School of Ecology and Conservation, Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bengaluru, 560065, India.|Ashoka Trust for Research in Ecology and the Environment, Royal Enclave, Sriramapura, Jakkur, Bengaluru, 560064, India.
authors.forename: Ramanan
authors.initials: R
authors.lastname: Uma Shaanker
authors.affiliation: DST Unit of Nanoscience and Thematic Unit of Excellence, Department of Chemistry, Indian Institute of Technology Madras, Chennai, 600036, India.
authors.forename: Thalappil
authors.initials: T
authors.lastname: Pradeep
mesh_terms: D000107:Acetylation; D002867:Chromones / Q000032:analysis*; D006031:Glycosylation; D029582:Meliaceae / Q000737:chemistry / Q000648:ultrastructure*; D053858:Metabolic Networks and Pathways; D010880:Piperidines / Q000032:analysis*; D024301:Plant Bark / Q000737:chemistry / Q000648:ultrastructure; D018547:Plant Stems / Q000737:chemistry / Q000648:ultrastructure; D036226:Seedlings / Q000737:chemistry / Q000648:ultrastructure*; D012639:Seeds / Q000737:chemistry / Q000648:ultrastructure; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014018:Tissue Distribution
chemical_list: C573233:5,7-dihydroxy-2-methyl-8-(4-(3-hydroxy-1-methyl)-piperidinyl)-4H-1-benzopyran-4-one; D002867:Chromones; D010880:Piperidines