title: Different RNA splicing mechanisms contribute to diverse infective outcome of classical swine fever viruses of differing virulence: insights from the deep sequencing data in swine umbilical vein endothelial cells.
abstract: Molecular mechanisms underlying RNA splicing regulation in response to viral infection are poorly understood. Classical swine fever (CSF), one of the most economically important and highly contagious swine diseases worldwide, is caused by classical swine fever virus (CSFV). Here, we used high-throughput sequencing to obtain the digital gene expression (DGE) profile in swine umbilical vein endothelial cells (SUVEC) to identify different response genes for CSFV by using both Shimen and C strains. The numbers of clean tags obtained from the libraries of the control and both CSFV-infected libraries were 3,473,370, 3,498,355, and 3,327,493 respectively. In the comparison among the control, CSFV-C, and CSFV-Shimen groups, 644, 158, and 677 differentially expressed genes (DEGs) were confirmed in the three groups. Pathway enrichment analysis showed that many of these DEGs were enriched in spliceosome, ribosome, proteasome, ubiquitin-mediated proteolysis, cell cycle, focal adhesion, Wnt signalling pathway, etc., where the processes differ between CSFV strains of differing virulence. To further elucidate important mechanisms related to the differential infection by the CSFV Shimen and C strains, we identified four possible profiles to assess the significantly expressed genes only by CSFV Shimen or CSFV C strain. GO analysis showed that infection with CSFV Shimen and C strains disturbed 'RNA splicing' of SUVEC, resulting in differential 'gene expression' in SUVEC. Mammalian target of rapamycin (mTOR) was identified as a significant response regulator contributed to impact on SUVEC function for CSFV Shimen. This computational study suggests that CSFV of differing virulence could induce alterations in RNA splicing regulation in the host cell to change cell metabolism, resulting in acute haemorrhage and pathological damage or infectious tolerance.
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi; School of Life Science and Technology, Xidian University, Xi'an, China.
authors.forename: Pengbo
authors.initials: P
authors.lastname: Ning
authors.affiliation: College of Science, Northwest A&F University , Yangling , China.
authors.forename: Yulu
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: College of Veterinary Medicine, Northwest A&F University , Yangling , Shaanxi.
authors.forename: Wulong
authors.initials: W
authors.lastname: Liang
authors.affiliation: College of Veterinary Medicine, Northwest A&F University , Yangling , Shaanxi.
authors.forename: Yanming
authors.initials: Y
authors.lastname: Zhang
keywords: CSFV C; CSFV Shimen; RNA splicing; SUVEC; mTOR