title: Next generation sequencing: Improved resolution for paternal/maternal duos analysis.
abstract: In the case of two mismatches observed in alleged parent-offspring pairs, there is doubt as to whether there is an exclusion of the putative parent or the existence of two mutations. Here, we report on four cases with two mismatches in paternal/maternal duos based on capillary electrophoresis (CE) results. The analyzed next generation sequencing (NGS) results were compared with 20 autosomal STRs derived from previous CE-based analysis. In summary, the NGS samples used offered comprehensive information of different types of markers that can improve resolutions for paternal/maternal duos analysis.
authors.affiliation: Institute of Forensic Science, Tianjin Public Security Bureau, Tianjin 300384, China. Electronic address: mayan0615@hotmail.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Institute of Forensic Science, Tianjin Public Security Bureau, Tianjin 300384, China.
authors.forename: Jin-Zhi
authors.initials: JZ
authors.lastname: Kuang
authors.affiliation: Institute of Forensic Science, Tianjin Public Security Bureau, Tianjin 300384, China.
authors.forename: Tong-Gang
authors.initials: TG
authors.lastname: Nie
authors.affiliation: Institute of Forensic Science, Tianjin Public Security Bureau, Tianjin 300384, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Institute of Forensic Science, Tianjin Public Security Bureau, Tianjin 300384, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Yang
mesh_terms: D041321:Chromosomes, Human, X; D019075:Electrophoresis, Capillary; D005260:Female; D005819:Genetic Markers; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D009035:Mothers; D009154:Mutation; D010334:Paternity; D016133:Polymerase Chain Reaction
chemical_list: D005819:Genetic Markers
keywords: Next generation sequencing (NGS); Paternal/maternal duos analysis; forensic geneticsForensic genetics