title: Transcriptional regulation with CRISPR-Cas9: principles, advances, and applications.
abstract: CRISPR-Cas9 has recently emerged as a promising system for multiplexed genome editing as well as epigenome and transcriptome perturbation. Due to its specificity, ease of use and highly modular programmable nature, it has been widely adopted for a variety of applications such as genome editing, transcriptional inhibition and activation, genetic screening, DNA localization imaging, and many more. In this review, we will discuss non-editing applications of CRISPR-Cas9 for transcriptome perturbation, metabolic engineering, and synthetic biology.
authors.affiliation: BioCircuits Institute, University of California San Diego, La Jolla, CA 92093, USA; San Diego Center for Systems Biology, University of California San Diego, La Jolla, CA 92093, USA.
authors.forename: Andriy
authors.initials: A
authors.lastname: Didovyk
authors.affiliation: BioCircuits Institute, University of California San Diego, La Jolla, CA 92093, USA; San Diego Center for Systems Biology, University of California San Diego, La Jolla, CA 92093, USA.
authors.forename: Bartłomiej
authors.initials: B
authors.lastname: Borek
authors.affiliation: BioCircuits Institute, University of California San Diego, La Jolla, CA 92093, USA; San Diego Center for Systems Biology, University of California San Diego, La Jolla, CA 92093, USA. Electronic address: ltsimring@ucsd.edu.
authors.forename: Lev
authors.initials: L
authors.lastname: Tsimring
authors.affiliation: BioCircuits Institute, University of California San Diego, La Jolla, CA 92093, USA; San Diego Center for Systems Biology, University of California San Diego, La Jolla, CA 92093, USA; Department of Bioengineering, University of California San Diego, La Jolla, CA 92093, USA; Molecular Biology Section, Division of Biological Sciences, University of California San Diego, La Jolla, CA 92093, USA. Electronic address: jhasty@eng.ucsd.edu.
authors.forename: Jeff
authors.initials: J
authors.lastname: Hasty
mesh_terms: D000818:Animals; D064113:CRISPR-Cas Systems / Q000235:genetics*; D000072669:Gene Editing; D005786:Gene Expression Regulation; D006801:Humans; D014158:Transcription, Genetic