title: Water-soluble fractions from defatted sesame seeds protect human neuroblast cells against peroxyl radicals and hydrogen peroxide-induced oxidative stress.
abstract: Oxidative stress is involved in the development of aging-related diseases, such as neurodegenerative diseases. Dietary antioxidants that can protect neuronal cells from oxidative damage play an important role in preventing such diseases. Previously, we reported that water-soluble fractions purified from defatted sesame seed flour exhibit good antioxidant activity in vitro. In the present study, we investigated the protective effects of white and gold sesame seed water-soluble fractions (WS-wsf and GS-wsf, respectively) against 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH) and hydrogen peroxide (H2O2) induced oxidative stress in human neuroblast SH-SY5Y cells. Pretreatment with WS-wsf and GS-wsf did not protect cells against AAPH-induced cytotoxicity, while simultaneous co-treatment with AAPH significantly improved cell viability and inhibited membrane lipid peroxidation. These results suggest that WS-wsf and GS-wsf protect cells from AAPH-induced extracellular oxidative damage via direct scavenging of peroxyl radicals. When oxidative stress was induced by H2O2, pretreatment WS-wsf and GS-wsf significantly enhanced cell viability. These results suggest that in addition to radical scavenging, WS-wsf and GS-wsf enhance cellular resistance to intracellular oxidative stress by activation of the Nrf-2/ARE pathway as confirmed by the increased Nrf2 protein level in the nucleus and increased heme oxygenase 1 (HO-1) mRNA expression. The roles of ferulic and vanillic acids as bioactive antioxidants in these fractions were also confirmed. In conclusion, our results indicated that WS-wsf and GS-wsf, which showed antioxidant activity in vitro, are also efficient antioxidants in a cell system protecting SH-SY5Y cells against both extracellular and intracellular oxidative stress.
authors.affiliation: a United Graduate School of Agricultural Science , Gifu University , Gifu , Japan ;
authors.forename: Sana
authors.initials: S
authors.lastname: Ben Othman
authors.affiliation: a United Graduate School of Agricultural Science , Gifu University , Gifu , Japan ;
authors.forename: Nakako
authors.initials: N
authors.lastname: Katsuno
authors.affiliation: b Shinsei Co. Ltd. , Kitanagoya , Aichi , Japan.
authors.forename: Akemi
authors.initials: A
authors.lastname: Kitayama
authors.affiliation: b Shinsei Co. Ltd. , Kitanagoya , Aichi , Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Fujimura
authors.affiliation: a United Graduate School of Agricultural Science , Gifu University , Gifu , Japan ;
authors.forename: Kohji
authors.initials: K
authors.lastname: Kitaguchi
authors.affiliation: a United Graduate School of Agricultural Science , Gifu University , Gifu , Japan ;
authors.forename: Tomio
authors.initials: T
authors.lastname: Yabe
mesh_terms: D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D015227:Lipid Peroxidation; D018384:Oxidative Stress; D010545:Peroxides; D012639:Seeds / Q000378:metabolism*; D031685:Sesamum / Q000378:metabolism*; D014867:Water / Q000737:chemistry*
chemical_list: D010545:Peroxides; D014867:Water; C049375:perhydroxyl radical; D006861:Hydrogen Peroxide
keywords: AAPH; Nrf2/ARE; SH-SY5Y cell; membrane lipid peroxidation; oxidative stress; Sesamum indicum L.