title: "Smart" molecularly imprinted monoliths for the selective capture and easy release of proteins.
abstract: A new thermally switchable molecularly imprinted monolith for the selective capture and release of proteins has been designed. First, a generic poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolith reacted with ethylenediamine followed by functionalization with 2-bromoisobutyryl bromide to introduce the initiator for atom transfer radical polymerization. Subsequently, a protein-imprinted poly(N-isopropylacrylamide) layer was grafted onto the surface of the monolithic matrix by atom transfer radical polymerization. Scanning electron microscopy and energy-dispersive X-ray spectroscopy of the cross-sections of imprinted monoliths confirmed the formation of dense poly(N-isopropylacrylamide) brushes on the pore surface. The imprinted monolith exhibited high specificity and selectivity toward its template protein myoglobin over competing proteins and a remarkably large maximum adsorption capacity of 1641 mg/g. Moreover, this "smart" imprinted monolith featured thermally responsive characteristics that enabled selective capture and easy release of proteins triggered only by change in temperature with water as the mobile phase and avoided use of stronger organic solvents or change in ionic strength and pH.
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China.
authors.forename: Liyin
authors.initials: L
authors.lastname: Wen
authors.affiliation: College of Chemistry, Jilin University, Changchun, China.
authors.forename: Xinyi
authors.initials: X
authors.lastname: Tan
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Sun
authors.affiliation: Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing, China.
authors.forename: Frantisek
authors.initials: F
authors.lastname: Svec
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China.
authors.forename: Yongqin
authors.initials: Y
authors.lastname: Lv
mesh_terms: D000327:Adsorption; D002845:Chromatography / Q000295:instrumentation*; D004852:Epoxy Compounds / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008689:Methacrylates / Q000737:chemistry; D008768:Methylmethacrylates / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting; D009211:Myoglobin / Q000737:chemistry* / Q000302:isolation & purification; D009994:Osmolar Concentration; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D013696:Temperature
chemical_list: D004852:Epoxy Compounds; D008689:Methacrylates; D008768:Methylmethacrylates; D009211:Myoglobin; D011108:Polymers; C020347:GMA-EDMA; C004919:ethylene dimethacrylate; C007870:glycidyl methacrylate
keywords: Molecularly imprinted polymers; Monoliths; Proteins; Selective capture; Thermal switching