title: Enhanced Butanol Production Through Adding Organic Acids and Neutral Red by Newly Isolated Butanol-Tolerant Bacteria.
abstract: As alternative microorganisms for butanol production with high butanol tolerant and productivity are in high demand, one excellent butanol-tolerant bacterium, S10, was isolated and identified as Clostridium acetobutylicum S10. In order to enhance the performance of butanol production, organic acids and neutral red were added during butanol fermentation. Synergistic effects were exhibited in the combinations of organic acids and neutral red to promote butanol production. Consequently, the optimal concentrations of combined acetate, butyrate, and neutral red were determined at sodium acetate 1.61 g/L, sodium butyrate 1.88 g/L, and neutral red 0.79 g/L, respectively, with the butanol yield of 6.09 g/L which was 20.89 % higher than that in control. These results indicated that combination of adding organic acid and neutral red is a potential effective measure to improve butanol production.
authors.affiliation: School of Life Science and Technology, Harbin Institute of Technology, Harbin, 150001, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Jiang
authors.affiliation: School of Life Science and Technology, Harbin Institute of Technology, Harbin, 150001, China.|State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, 150090, China.
authors.forename: Guangli
authors.initials: G
authors.lastname: Cao
authors.affiliation: School of Life Science and Technology, Harbin Institute of Technology, Harbin, 150001, China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Wang
authors.affiliation: School of Life Science and Technology, Harbin Institute of Technology, Harbin, 150001, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Life Science and Technology, Harbin Institute of Technology, Harbin, 150001, China.
authors.forename: Jinzhu
authors.initials: J
authors.lastname: Song
authors.affiliation: School of Life Science and Technology, Harbin Institute of Technology, Harbin, 150001, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Cong
authors.affiliation: School of Chemical Engineering and Technology, Harbin Institute of Technology, Harbin, 150001, China.
authors.forename: Junzheng
authors.initials: J
authors.lastname: Zhang
authors.affiliation: School of Life Science and Technology, Harbin Institute of Technology, Harbin, 150001, China. microbio207@gmail.com.|State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, 150090, China. microbio207@gmail.com.
authors.forename: Qian
authors.initials: Q
authors.lastname: Yang
mesh_terms: D000704:Analysis of Variance; D001709:Biotechnology / Q000379:methods*; D000440:Butanols / Q000378:metabolism*; D002264:Carboxylic Acids / Q000494:pharmacology*; D046969:Clostridium acetobutylicum / Q000302:isolation & purification* / Q000378:metabolism; D005285:Fermentation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D009499:Neutral Red / Q000494:pharmacology*; D010802:Phylogeny
chemical_list: D000440:Butanols; D002264:Carboxylic Acids; D009499:Neutral Red
keywords: Butanol; Clostridium acetobutylicum; Neutral red; Organic acid; Tolerance