title: Proteomic analysis of the skin from Chinese fire-bellied newt and comparison to Chinese giant salamander.
abstract: Animal skin that directly interfaces with the external environment has developed diverse adaptive functions to a variety of ecological conditions laden with pathogenic infection and physical harm. Amphibians exhibit various adaptations related to their "incomplete" shift from the aquatic to the terrestrial habitat. Therefore, it is very necessary to explore the molecular basis of skin function and adaptation in amphibians. Currently, the studies on the molecular mechanisms of skin functions in anuran amphibians have been reported, but in urodele amphibians are rare. This study identified the skin proteomes of Chinese fire-bellied newt Cynops orientalis by a proteomic method, and compared the results to the skin proteomes of Chinese giant salamander Andrias davidianus obtained previously. A total of 452 proteins were identified in the newt skin by MALDI-TOF/MS, and functional annotation results by DAVID analysis showed that special functions such as wound healing, immune response, defense and respiration, were significantly enriched. Comparison results showed that the two species had a great difference in the aspects of protein kinds and abundance, and the highly expressed proteins may tightly correlate with living conditions. Moreover, the newt skin might have stronger immunity, but weaker respiration than the giant salamander skin to adapt to various living environments. This research provides a molecular basis for further studies on amphibian skin function and adaptation.
authors.affiliation: State Key Laboratory Cultivation Base for Cell Differentiation Regulation and Henan Engineering Laboratory for Bioengineering and Drug Development, College of Life Science, Henan Normal University, Xinxiang 453007, China.
authors.forename: Jingyan
authors.initials: J
authors.lastname: Sun
authors.affiliation: State Key Laboratory Cultivation Base for Cell Differentiation Regulation and Henan Engineering Laboratory for Bioengineering and Drug Development, College of Life Science, Henan Normal University, Xinxiang 453007, China.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Geng
authors.affiliation: State Key Laboratory Cultivation Base for Cell Differentiation Regulation and Henan Engineering Laboratory for Bioengineering and Drug Development, College of Life Science, Henan Normal University, Xinxiang 453007, China.
authors.forename: Jianlin
authors.initials: J
authors.lastname: Guo
authors.affiliation: State Key Laboratory Cultivation Base for Cell Differentiation Regulation and Henan Engineering Laboratory for Bioengineering and Drug Development, College of Life Science, Henan Normal University, Xinxiang 453007, China.
authors.forename: Xiayan
authors.initials: X
authors.lastname: Zang
authors.affiliation: State Key Laboratory Cultivation Base for Cell Differentiation Regulation and Henan Engineering Laboratory for Bioengineering and Drug Development, College of Life Science, Henan Normal University, Xinxiang 453007, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Li
authors.affiliation: State Key Laboratory Cultivation Base for Cell Differentiation Regulation and Henan Engineering Laboratory for Bioengineering and Drug Development, College of Life Science, Henan Normal University, Xinxiang 453007, China.
authors.forename: Deming
authors.initials: D
authors.lastname: Li
authors.affiliation: State Key Laboratory Cultivation Base for Cell Differentiation Regulation and Henan Engineering Laboratory for Bioengineering and Drug Development, College of Life Science, Henan Normal University, Xinxiang 453007, China. Electronic address: cellkeylab@126.com.
authors.forename: Cunshuan
authors.initials: C
authors.lastname: Xu
mesh_terms: D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D012454:Salamandra / Q000254:growth & development / Q000378:metabolism*; D012455:Salamandridae / Q000254:growth & development / Q000378:metabolism*; D012867:Skin / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D020543:Proteome
keywords: Defense; Giant salamander; Immune; Newt; Proteome; Skin