title: Nanocomposite Hydrogels as Platform for Cells Growth, Proliferation, and Chemotaxis.
abstract: The challenge of mimicking the extracellular matrix with artificial scaffolds that are able to reduce immunoresponse is still unmet. Recent findings have shown that mesenchymal stem cells (MSC) infiltrating into the implanted scaffold have effects on the implant integration by improving the healing process. Toward this aim, a novel polyamidoamine-based nanocomposite hydrogel is synthesized, cross-linked with porous nanomaterials (i.e., mesoporous silica nanoparticles), able to release chemokine proteins. A comprehensive viscoelasticity study confirms that the hydrogel provides optimal structural support for MSC infiltration and proliferation. The efficiency of this hydrogel, containing the chemoattractant stromal cell-derived factor 1α (SDF-1α), in promoting MSC migration in vitro is demonstrated. Finally, subcutaneous implantation of SDF-1α-releasing hydrogels in mice results in a modulation of the inflammatory reaction. Overall, the proposed SDF-1α-nanocomposite hydrogel proves to have potential for applications in tissue engineering.
authors.affiliation: Institut de Science et d'Ingénierie Supramoléculaires, Université de Strasbourg, 8 rue Gaspard Monge, 67000, Strasbourg, France.
authors.forename: Federica
authors.initials: F
authors.lastname: Fiorini
authors.affiliation: Institut de Science et d'Ingénierie Supramoléculaires, Université de Strasbourg, 8 rue Gaspard Monge, 67000, Strasbourg, France.
authors.forename: Eko Adi
authors.initials: EA
authors.lastname: Prasetyanto
authors.affiliation: Department of Nanomedicine, Houston Methodist Research Institute, 6670 Bertner Avenue, Houston, TX, 77030, USA.
authors.forename: Francesca
authors.initials: F
authors.lastname: Taraballi
authors.affiliation: Department of Nanomedicine, Houston Methodist Research Institute, 6670 Bertner Avenue, Houston, TX, 77030, USA.|College of Materials Science and Engineering, University of Chinese Academy of Science, 19A Yuquanlu, Beijing, 100049, China.
authors.forename: Laura
authors.initials: L
authors.lastname: Pandolfi
authors.affiliation: Departamento de Química Física I Universidad Complutense, Ciudad Universitaria s/n, 28040, Madrid, Spain.|Instituto de Investigacion Hospital 12 de Octubre (i+12), Avda. de Cordoba s/n, 28041, Madrid, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Monroy
authors.affiliation: Departamento de Química Física I Universidad Complutense, Ciudad Universitaria s/n, 28040, Madrid, Spain.|Instituto de Investigacion Hospital 12 de Octubre (i+12), Avda. de Cordoba s/n, 28041, Madrid, Spain.
authors.forename: Iván
authors.initials: I
authors.lastname: López-Montero
authors.affiliation: Department of Nanomedicine, Houston Methodist Research Institute, 6670 Bertner Avenue, Houston, TX, 77030, USA.
authors.forename: Ennio
authors.initials: E
authors.lastname: Tasciotti
authors.affiliation: Institut de Science et d'Ingénierie Supramoléculaires, Université de Strasbourg, 8 rue Gaspard Monge, 67000, Strasbourg, France. decola@unistra.fr.
authors.forename: Luisa
authors.initials: L
authors.lastname: De Cola
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001854:Bone Marrow Cells / Q000166:cytology; D049109:Cell Proliferation / Q000187:drug effects; D054377:Chemokine CXCL12 / Q000494:pharmacology; D002633:Chemotaxis / Q000187:drug effects; D020100:Hydrogels / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D053761:Nanocomposites / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D016062:Porosity; D012212:Rheology; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D054377:Chemokine CXCL12; D020100:Hydrogels; D012822:Silicon Dioxide
keywords: chemotaxis; hydrogel; in vivo implant; mesoporous particles; nanocomposites