title: Effects of Salt Stress on Carbohydrate Metabolism of Lactobacillus plantarum ATCC 14917.
abstract: Lactic acid bacteria are widely used in fermented foods, especially cheese products. In this study, we observed the salt tolerance of Lactobacillus plantarum ATCC 14917 after exposure to different concentrations of NaCl in MRS medium. Quantitative proteomic profiles using two-dimensional electrophoresis identified 384 proteins, of which 26 were upregulated and 31 downregulated. Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry was then used to identify 11 proteins, of which three were linked to carbohydrate metabolism. The downregulation of carbamoyl phosphate synthase in carbohydrate metabolism revealed a bacterial regulation mechanism to save energy in order to survive during the salt tolerance. Other proteins were found involved in transcription-translation processes, fatty acid biosynthesis, and the primary metabolic process.
authors.affiliation: Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, Ningbo University, Ningbo, 315211, Zhejiang, China.
authors.forename: Pingping
authors.initials: P
authors.lastname: Wang
authors.affiliation: Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, Ningbo University, Ningbo, 315211, Zhejiang, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, Ningbo University, Ningbo, 315211, Zhejiang, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
authors.affiliation: Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, Ningbo University, Ningbo, 315211, Zhejiang, China. daodongpan@163.com.|Department of Food Science and Nutrition, Jinling College, Nanjing Normal University, Nanjing, 210097, Jiangsu, China. daodongpan@163.com.
authors.forename: Daodong
authors.initials: D
authors.lastname: Pan
authors.affiliation: Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, Ningbo University, Ningbo, 315211, Zhejiang, China.
authors.forename: Xiaoqun
authors.initials: X
authors.lastname: Zeng
authors.affiliation: Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, Ningbo University, Ningbo, 315211, Zhejiang, China.
authors.forename: Kemeng
authors.initials: K
authors.lastname: Cheng
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D050260:Carbohydrate Metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005227:Fatty Acids / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D048191:Lactiplantibacillus plantarum / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D012965:Sodium Chloride / Q000032:analysis / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001426:Bacterial Proteins; D005227:Fatty Acids; D012965:Sodium Chloride