title: Administration of activated glial condition medium in the nucleus accumbens extended extinction and intensified reinstatement of methamphetamine-induced conditioned place preference.
abstract: Methamphetamine (METH) is a psychostimulant drug with significant abuse potential and neurotoxic effects. A high percentage of users relapse to use after detoxification and no effective medication has been developed for treatment of METH addiction. Developing evidences indicated the role of glial cells in drugs abused related phenomena. However, little is known about the role of these cells in the maintenance and reinstatement of METH-seeking behaviors. Therefore, the current study was conducted to clarify the role of glial cells in the maintenance and reinstatement of METH-induced conditioned place preference (CPP) in rats. Astrocyte condition medium (ACM) and neuroglia conditioned medium (NCM) are liquid mediums prepared from primary astrocyte and neuroglia cells. These mediums seem to contain many factors that release by glia cells. CPP was induced by systemic administration of METH (1mg/kg for 5days, s.c.). Following the establishment of CPP, the rats were given daily bilateral injections (0.5μl/side) of either vehicle, ACM or NCM into the nucleus accumbens (NAc) and then were tested for the maintenance and reinstatement. Intra-NAc administration of ACM treated with METH, could extend the extinction period and also, intensified the magnitude of METH reinstatement. Furthermore, intra-accumbal administration of NCM treated with METH notably delayed the extinction period by four days and significantly increased the magnitude of CPP score in the reinstatement phase compared to the post-test phase. Collectively, these findings suggested that activation of glial cells may be involved in the maintenance and reinstatement of METH-seeking behaviors. It provides new evidence that glia cells might be considered as a potential target for the treatment of METH addiction.
authors.affiliation: Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Addiction Department, Center of Excellence in Psychiatry and Clinical Psychology, School of Behavioral Sciences and Mental Health (Institute of Tehran Psychiatry), Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Arezoomandan
authors.affiliation: Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Marzieh
authors.initials: M
authors.lastname: Moradi
authors.affiliation: Molecular Medicine Research Centre, Hormozgan University of Medical Sciences, Bandar Abbas, Iran; Iran National Science Foundation, Tehran, Iran.
authors.forename: Ghassem
authors.initials: G
authors.lastname: Attarzadeh-Yazdi
authors.affiliation: Department of Physiological Sciences, University of Brasilia, Brasilia, Brazil.
authors.forename: Carlos
authors.initials: C
authors.lastname: Tomaz
authors.affiliation: Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Iran National Science Foundation, Tehran, Iran. Electronic address: Haghparast@sbmu.ac.ir.
authors.forename: Abbas
authors.initials: A
authors.lastname: Haghparast
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000697:Central Nervous System Stimulants / Q000494:pharmacology; D002540:Cerebral Cortex / Q000166:cytology; D003216:Conditioning, Operant / Q000187:drug effects*; D017077:Culture Media, Conditioned / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D005108:Extinction, Psychological / Q000187:drug effects*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D008297:Male; D008694:Methamphetamine / Q000494:pharmacology; D008845:Microinjections; D009457:Neuroglia / Q000737:chemistry / Q000378:metabolism; D009714:Nucleus Accumbens / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D000697:Central Nervous System Stimulants; D017077:Culture Media, Conditioned; D005904:Glial Fibrillary Acidic Protein; D008694:Methamphetamine
keywords: Conditioned place preference; Glial cell; Methamphetamine; Nucleus accumbens; Reinstatement; Reward