title: Cytosolic superoxide dismutase can provide protection against Fasciola gigantica.
abstract: Superoxide dismutases (SOD), antioxidant metallo-enzymes, are a part of the first line of defense in the trematode parasites which act as the chief scavengers for reactive oxygen species (ROS). A recombinant Fasciola gigantica cytosolic SOD (FgSOD) was expressed in Escherichia coli BL21 (DE3) and used for immunizing rabbits to obtain polyclonal antibodies (anti-rFgSOD). This rabbit anti-rFgSOD reacted with the native FgSOD at a molecular weight of 17.5kDa. The FgSOD protein was expressed at high level in parenchyma, caecal epithelium and egg of the parasite. The rFgSOD reacted with antisera from rabbits infected with F. gigantica metacercariae collected at 2, 5, and 7 weeks after infection, and reacted with sera of infected mice. Anti-rFgSOD exhibited cross reactivity with the other parasites' antigens, including Eurytrema pancreaticum, Cotylophoron cotylophorum, Fischoederius cobboldi, Gastrothylax crumenifer, Paramphistomum cervi, and Setaria labiato papillosa. A vaccination was performed in imprinting control region (ICR) mice by subcutaneous injection with 50μg of rFgSOD combined with Freund's adjuvant. At 2 weeks after the second boost, mice were infected with 15 metacercariae by oral route. IgG1 and IgG2a in the immune sera were determined to indicate Th2 and Th1 immune responses. It was found that the parasite burden was reduced by 45%, and both IgG1 and IgG2a levels showed correlation with the numbers of worm recoveries.
authors.affiliation: Department of Pathobiology, Faculty of Science, Mahidol University, Rama VI Rd, Ratchathewi, Bangkok 10400, Thailand.
authors.forename: Wipaphorn
authors.initials: W
authors.lastname: Jaikua
authors.affiliation: Faculty of Allied Health Sciences, Burapha University, Long-Hard Bangsaen Rd, SaenSook Sub-District, Mueang District, Chonburi 20131, Thailand.
authors.forename: Pornanan
authors.initials: P
authors.lastname: Kueakhai
authors.affiliation: Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Rd, Ratchathewi, Bangkok 10400, Thailand.
authors.forename: Kulathida
authors.initials: K
authors.lastname: Chaithirayanon
authors.affiliation: Department of Pathobiology, Faculty of Science, Mahidol University, Rama VI Rd, Ratchathewi, Bangkok 10400, Thailand.
authors.forename: Rataya
authors.initials: R
authors.lastname: Tanomrat
authors.affiliation: Department of Pathobiology, Faculty of Science, Mahidol University, Rama VI Rd, Ratchathewi, Bangkok 10400, Thailand.
authors.forename: Sirima
authors.initials: S
authors.lastname: Wongwairot
authors.affiliation: Department of Pathobiology, Faculty of Science, Mahidol University, Rama VI Rd, Ratchathewi, Bangkok 10400, Thailand.
authors.forename: Suda
authors.initials: S
authors.lastname: Riengrojpitak
authors.affiliation: Faculty of Allied Health Sciences, Burapha University, Long-Hard Bangsaen Rd, SaenSook Sub-District, Mueang District, Chonburi 20131, Thailand; Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Rd, Ratchathewi, Bangkok 10400, Thailand.
authors.forename: Prasert
authors.initials: P
authors.lastname: Sobhon
authors.affiliation: Faculty of Allied Health Sciences, Burapha University, Long-Hard Bangsaen Rd, SaenSook Sub-District, Mueang District, Chonburi 20131, Thailand. Electronic address: narin_bio@hotmail.com.
authors.forename: Narin
authors.initials: N
authors.lastname: Changklungmoa
mesh_terms: D000328:Adult; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D003429:Cross Reactions; D003600:Cytosol / Q000276:immunology / Q000378:metabolism*; D005209:Fasciola / Q000276:immunology*; D005211:Fascioliasis / Q000097:blood / Q000276:immunology*; D005620:Freund's Adjuvant / Q000627:therapeutic use; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D058488:Metacercariae / Q000469:parasitology*; D051379:Mice; D011817:Rabbits; D011994:Recombinant Proteins / Q000097:blood / Q000276:immunology*; D013482:Superoxide Dismutase / Q000276:immunology* / Q000378:metabolism* / Q000627:therapeutic use
chemical_list: D000909:Antibodies, Helminth; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D005620:Freund's Adjuvant; D013482:Superoxide Dismutase
keywords: Antioxidant enzyme; Fasciola gigantica; Parasite; Superoxide dismutase; Vaccine