title: [Studies on immunogenicity and immunoprotection induced by heat shock protein 60 kDa of Schistosoma japonicum in mice].
abstract: OBJECTIVE
  To evaluate the immunogenicity and immunoprotective effect of heat shock protein 60 kDa (SjHSP60) of Schistosoma japonicum in mice after immunization and challenge infection, and explore the mechanism.
  
  
  METHODS
  B cell/antibody-related databases and analysis tools were used to predict B-cell epitopes of SjHSP60. The mice were immunized with the recombinant SjHSP60 and challenged with S. japonicum cercariae. SjHSP60-specific antibodies in serum were detected by ELISA. The level of splenocyte proliferation was determined by ³H-TdR incorporation. Ex vivo suppression assay was performed to investigate the effects of CD4⁺CD25⁺ regulatory T cells (Tregs) induced by SjHSP60.
  
  
  RESULTS
  SjHSP60 possessed multiple predominant regions of B-cell epitopes. SjHSP60 induced a significant increase in both SjHSP60-specific IgG levels (P < 0.01) and splenocyte proliferation (P < 0.01) with a higher IFN-γ production (P < 0.01). However, the immunization with SjHSP60 resulted no significant reduction in adult worms (P > 0.05) and liver-accumulated eggs (P > 0.05) in S. japonicum-infected mice. Ex vivo assay showed that CD4⁺CD25⁺ Tregs from SjHSP60-immunized mice enhanced immunosuppressive activity.
  
  
  CONCLUSIONS
  SjHSP60 has a dual role in host immune system, being involved in the induction of dominant humoral and cellular immune responses as well as in the enhancement of immunosuppression.
authors.forename: Xin
authors.initials: X
authors.lastname: Jin
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Chen
authors.forename: Ji-feng
authors.initials: JF
authors.lastname: Zhu
authors.forename: Zhi-peng
authors.initials: ZP
authors.lastname: Xu
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.forename: Sha
authors.initials: S
authors.lastname: Zhou
authors.forename: Chuan
authors.initials: C
authors.lastname: Su
mesh_terms: D000818:Animals; D018834:Chaperonin 60 / Q000276:immunology*; D018985:Epitopes, B-Lymphocyte; D005260:Female; D007108:Immune Tolerance; D007371:Interferon-gamma / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D024101:Mitochondrial Proteins / Q000276:immunology*; D012549:Schistosoma japonicum / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
chemical_list: D018834:Chaperonin 60; D018985:Epitopes, B-Lymphocyte; C557066:Hspd1 protein, mouse; D024101:Mitochondrial Proteins; D007371:Interferon-gamma