title: Afamin promotes glucose metabolism in papillary thyroid carcinoma.
abstract: Circulating afamin (AFM) concentrations have been investigated as a tumor biomarker in various types of carcinomas. However, suitable cell lines expressing human afamin have not yet been reported and current knowledge of the functions of afamin, particularly at the mechanistic molecular level, is very limited. In the current study, thyroid cancer cell lines 8505c and K1 were used to investigate the potential functions of afamin. AFM over-expression models and vector controls of 8505c (8505c + AFM and 8505c + NC) and K1 (K1 + AFM and K1 + NC) were successfully established by Lenti-LV5-AFM and Lenti-LV5-NC transfection. The change of gene expression was detected by qRT-PCR and western blotting analysis. (18)F-FDG imaging in xenografts model was performed using a micro PET/CT. We found that protein level of GAPDH, GLUT1, HK2, p-AKT, AKT, p-mTOR and PARP1 were up-regulated in K1 + AFM cells when compared to K1 and K1 + NC. While in 8505c, 8505c + NC and 8505c cells, the expression level of these genes were not significantly changed. (18)F-FDG uptake was much higher in K1 + AFM cells when compared to K1 and K1 + NC in vitro and in vivo. In conclusion, afamin could promote glycometabolism by up-regulating the glucose metabolism key enzymes in papillary thyroid carcinoma. These findings reveal new clues of the molecular function of AFM.
authors.affiliation: Department of Nuclear Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, PR China.
authors.forename: Chen-Tian
authors.initials: CT
authors.lastname: Shen
authors.affiliation: Department of Nuclear Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, PR China.
authors.forename: Wei-Jun
authors.initials: WJ
authors.lastname: Wei
authors.affiliation: Department of Nuclear Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, PR China.
authors.forename: Zhong-Ling
authors.initials: ZL
authors.lastname: Qiu
authors.affiliation: Department of Nuclear Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, PR China.
authors.forename: Hong-Jun
authors.initials: HJ
authors.lastname: Song
authors.affiliation: Department of Nuclear Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, PR China. Electronic address: lqyn@sh163.net.
authors.forename: Quan-Yong
authors.initials: QY
authors.lastname: Luo
mesh_terms: D000818:Animals; D002291:Carcinoma, Papillary / Q000000981:diagnostic imaging / Q000235:genetics / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism*; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D005947:Glucose / Q000378:metabolism*; D051272:Glucose Transporter Type 1 / Q000235:genetics / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics / Q000378:metabolism; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism*; D057809:HEK293 Cells; D006593:Hexokinase / Q000235:genetics / Q000378:metabolism; D006801:Humans; D051379:Mice; D009368:Neoplasm Transplantation; D000072078:Positron Emission Tomography Computed Tomography; D012709:Serum Albumin / Q000235:genetics* / Q000378:metabolism*; D000075462:Serum Albumin, Human; D015398:Signal Transduction; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000000981:diagnostic imaging / Q000235:genetics / Q000378:metabolism*
chemical_list: C088246:AFM protein, human; D002352:Carrier Proteins; D051272:Glucose Transporter Type 1; D006023:Glycoproteins; C495677:SLC2A1 protein, human; D012709:Serum Albumin; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; C000609700:HK2 protein, human; D006593:Hexokinase; D005947:Glucose; D000075462:Serum Albumin, Human
keywords: (18)F-FDG; Afamin; Gene expression; Glucose metabolism; Thyroid carcinoma