title: Molecular characterization and expression analyses of three RIG-I-like receptor signaling pathway genes (MDA5, LGP2 and MAVS) in Larimichthys crocea.
abstract: In this study, we sequenced and characterized melanoma differentiation-associated antigen 5 (LcMDA5), laboratory of genetics and physiology 2 (LcLGP2) and mitochondrial antiviral signaling protein (LcMAVS) from large yellow croaker (Larimichthys crocea). The LcMDA5 encodes 969 amino acids and contains two caspase-associated and recruitment domains (CARDs), a DExDc (DExD/H box-containing domain), a HELICc (helicase superfamily C-terminal domain) and a C-terminal regulatory domain (RD). The LcLGP2 encodes 679 amino acids and contains a DExDc, a HELICc and a RD. The LcMAVS encodes 512 amino acids and contains a CARD, a proline-rich domain, a transmembrane helix domain and a putative TRAF2-binding motif ((269)PVQDT(273)). Phylogenetic analyses showed that all the three genes of large yellow croaker are clustered together with their counterparts from other teleost fishes. The Real-time PCR analyses showed that all the three genes were found to be constitutively expressed in all examined tissues in large yellow croaker, but all with relatively low expression levels. Expression analyses showed that the three genes were all rapidly and significantly upregulated in vivo after poly (I:C) challenge in peripheral blood, liver, spleen and head kidney tissues. The results indicate that the LcMDA5, LcLGP2 and LcMAVS might play important roles in antiviral immune responses.
authors.affiliation: National Engineering Research Center of Marine Facilities Aquaculture, College of Marine Science, Zhejiang Ocean University, Zhoushan 316004, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Shen
authors.affiliation: National Engineering Research Center of Marine Facilities Aquaculture, College of Marine Science, Zhejiang Ocean University, Zhoushan 316004, China.
authors.forename: Yiwen
authors.initials: Y
authors.lastname: Hu
authors.affiliation: National Engineering Research Center of Marine Facilities Aquaculture, College of Marine Science, Zhejiang Ocean University, Zhoushan 316004, China.
authors.forename: Shuyi
authors.initials: S
authors.lastname: Zhang
authors.affiliation: National Engineering Research Center of Marine Facilities Aquaculture, College of Marine Science, Zhejiang Ocean University, Zhoushan 316004, China.
authors.forename: Jialang
authors.initials: J
authors.lastname: Zheng
authors.affiliation: National Engineering Research Center of Marine Facilities Aquaculture, College of Marine Science, Zhejiang Ocean University, Zhoushan 316004, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zeng
authors.affiliation: National Engineering Research Center of Marine Facilities Aquaculture, College of Marine Science, Zhejiang Ocean University, Zhoushan 316004, China.
authors.forename: Jianshe
authors.initials: J
authors.lastname: Zhang
authors.affiliation: National Engineering Research Center of Marine Facilities Aquaculture, College of Marine Science, Zhejiang Ocean University, Zhoushan 316004, China.
authors.forename: Aiyi
authors.initials: A
authors.lastname: Zhu
authors.affiliation: National Engineering Research Center of Marine Facilities Aquaculture, College of Marine Science, Zhejiang Ocean University, Zhoushan 316004, China. Electronic address: shenbinzjou@163.com.
authors.forename: Changwen
authors.initials: C
authors.lastname: Wu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology*; D007113:Immunity, Innate; D000072640:Interferon-Induced Helicase, IFIH1 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010473:Perciformes / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D011070:Poly I-C / Q000494:pharmacology; D011487:Protein Conformation; D020365:RNA Helicases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D016415:Sequence Alignment
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D029941:Fish Proteins; D000072640:Interferon-Induced Helicase, IFIH1; D020365:RNA Helicases; D011070:Poly I-C
keywords: Innate antiviral immunity; Large yellow croaker; Pattern recognition receptor; Poly (I:C); RT-qPCR