title: Active Site Characterization of Proteases Sequences from Different Species of Aspergillus.
abstract: A total of 129 proteases sequences comprising 43 serine proteases, 36 aspartic proteases, 24 cysteine protease, 21 metalloproteases, and 05 neutral proteases from different Aspergillus species were analyzed for the catalytically active site residues using MEROPS database and various bioinformatics tools. Different proteases have predominance of variable active site residues. In case of 24 cysteine proteases of Aspergilli, the predominant active site residues observed were Gln193, Cys199, His364, Asn384 while for 43 serine proteases, the active site residues namely Asp164, His193, Asn284, Ser349 and Asp325, His357, Asn454, Ser519 were frequently observed. The analysis of 21 metalloproteases of Aspergilli revealed Glu298 and Glu388, Tyr476 as predominant active site residues. In general, Aspergilli species-specific active site residues were observed for different types of protease sequences analyzed. The phylogenetic analysis of these 129 proteases sequences revealed 14 different clans representing different types of proteases with diverse active site residues.
authors.affiliation: Department of Biological Engineering, Inha University, Incheon, 42-751, Republic of Korea.
authors.forename: V K
authors.initials: VK
authors.lastname: Morya
authors.affiliation: Department of Molecular and Cellular Engineering, SHIATS, Allahabad, India.
authors.forename: Virendra K
authors.initials: VK
authors.lastname: Yadav
authors.affiliation: Department of Biotechnology, D.D.U. Gorakhpur University, Gorakhpur, 273009, India.
authors.forename: Sangeeta
authors.initials: S
authors.lastname: Yadav
authors.affiliation: Department of Biotechnology, D.D.U. Gorakhpur University, Gorakhpur, 273009, India. dinesh_yad@rediffmail.com.
authors.forename: Dinesh
authors.initials: D
authors.lastname: Yadav
mesh_terms: D000595:Amino Acid Sequence; D001230:Aspergillus / Q000145:classification / Q000201:enzymology*; D020134:Catalytic Domain; D030562:Databases, Protein; D010447:Peptide Hydrolases / Q000737:chemistry* / Q000378:metabolism; D010802:Phylogeny; D016415:Sequence Alignment
chemical_list: D010447:Peptide Hydrolases
keywords: Aspartic proteases; Aspergillus; Cysteine proteases; Metalloproteases; Serine proteases