title: Encapsulation of a Nerve Agent Detoxifying Enzyme by a Mesoporous Zirconium Metal-Organic Framework Engenders Thermal and Long-Term Stability.
abstract: Immobilized enzymes typically have greater thermal and operational stability than their soluble form. Here we report that for the first time, a nerve agent detoxifying enzyme, organophosphorus acid anhydrolase (OPAA), has been successfully encapsulated into a water-stable zirconium metal-organic framework (MOF). This MOF features a hierarchical mesoporous channel structure and exhibits a 12 wt % loading capacity of OPAA. The thermal and long-term stabilities of OPAA are both significantly enhanced after immobilization.
authors.affiliation: Department of Chemistry and Chemical and Biological Engineering, Northwestern University , 2145 Sheridan Road, Evanston, Illinois 60208, United States.
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Chemistry and Chemical and Biological Engineering, Northwestern University , 2145 Sheridan Road, Evanston, Illinois 60208, United States.
authors.forename: Su-Young
authors.initials: SY
authors.lastname: Moon
authors.affiliation: U.S. Army Edgewood Chemical Biological Center , 5183 Blackhawk Road, RDCB-DRC-C, Aberdeen Proving Ground, Maryland 21010-5424, United States.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Guelta
authors.affiliation: U.S. Army Edgewood Chemical Biological Center , 5183 Blackhawk Road, RDCB-DRC-C, Aberdeen Proving Ground, Maryland 21010-5424, United States.
authors.forename: Steven P
authors.initials: SP
authors.lastname: Harvey
authors.affiliation: Department of Chemistry and Chemical and Biological Engineering, Northwestern University , 2145 Sheridan Road, Evanston, Illinois 60208, United States.
authors.forename: Joseph T
authors.initials: JT
authors.lastname: Hupp
authors.affiliation: Department of Chemistry and Chemical and Biological Engineering, Northwestern University , 2145 Sheridan Road, Evanston, Illinois 60208, United States.|Department of Chemistry, Faculty of Science, King Abdulaziz University , Jeddah 21589, Saudi Arabia.
authors.forename: Omar K
authors.initials: OK
authors.lastname: Farha