title: A preliminary insight of correlation between human fecal microbial diversity and blood lipid profile.
abstract: The study aimed to evaluate the effect of human gut-derived lactic acid bacteria and yeast on cholesterol levels. Fecal samples from five healthy volunteers were examined for the level and diversity of dominant microbiota. Pichia kudriavzevii (QAUPK01, QAUPK02, QAUPK03, QAUPK04 and QAUPK05) and Candida tropicalis (QAUCT06) were identified by phenotypic methods and DNA sequencing and tested for in vitro cholesterol assimilation ability. Significant correlations (p < 0.05) between fecal microbial diversity, volunteers' age, body mass index (BMI) and serum lipid profile were established. From biochemical tests, eight strains of lactic acid bacteria (M1.1, M1.2, M2.1, M3.1, M3.2, M4.1, M5.1 and M5.2) were identified but no bsh activity was found in them. However, all yeast strains were able to assimilate cholesterol and maximum assimilation ability was shown by QAUPK03 (83.6%) and QAUPK05 (85.2%) after 72 h of growth at 37 °C.
authors.affiliation: a Department of Microbiology, Faculty of Biological Sciences , Quaid-i-Azam University, Islamabad , Pakistan ;
authors.forename: Ilyas Rana
authors.initials: IR
authors.lastname: Madeeha
authors.affiliation: b Armed Forces Institute of Pathology (AFIP), National University of Medical Sciences , Rawalpindi , Pakistan ;
authors.forename: Aamer
authors.initials: A
authors.lastname: Ikram
authors.affiliation: a Department of Microbiology, Faculty of Biological Sciences , Quaid-i-Azam University, Islamabad , Pakistan ;|c Unité de Recherche Aliments Bioprocédés Toxicologie Environnements EA , Normandie Université, UNICAEN , Caen , France.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Imran
mesh_terms: D000328:Adult; D000581:Amidohydrolases / Q000378:metabolism; D015992:Body Mass Index; D041022:Candida tropicalis / Q000302:isolation & purification; D008076:Cholesterol, HDL / Q000097:blood*; D008078:Cholesterol, LDL / Q000097:blood*; D004271:DNA, Fungal / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology*; D000069196:Gastrointestinal Microbiome; D041981:Gastrointestinal Tract / Q000382:microbiology; D006801:Humans; D007777:Lactobacillaceae / Q000302:isolation & purification; D008875:Middle Aged; D010802:Phylogeny; D010843:Pichia / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D014280:Triglycerides / Q000097:blood*; D055815:Young Adult
chemical_list: D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D004271:DNA, Fungal; D014280:Triglycerides; D000581:Amidohydrolases; C023154:choloylglycine hydrolase
keywords: Cholesterol assimilation; gut microbiota; lactic acid bacteria; yeast