title: The expression of cancerous inhibitor protein phosphatase 2A in chronic rhinosinusitis with nasal polyps.
abstract: CONCLUSIONS
  The study suggests that cancerous inhibitor of protein phosphatase 2A (CIP2A) expression and eosinophilia associate with chronic rhinosinusitis with nasal polyps with aspirin exacerbated respiratory disease (CRSwNP + AERD). Further studies with a larger sample size are needed to evaluate further the role of CIP2A and related pathways in CRSwNP + AERD.
  
  
  OBJECTIVE
  Low prostaglandin E2 levels putatively associate with CRSwNP + AERD and decreased c-Myc levels. The aim of this study was to evaluate the expression and revision-predictive role of oncoprotein CIP2A, another c-Myc modulator, in CRSwNP with/without AERD, and in antrochoanal polyps.
  
  
  METHODS
  Ninety retrospective archival objective glasses of nasal polyp tissue from CRSwNP or ACP patients were used for assessing mucosal eosinophilia. Of this population, 90 archival nasal polyp specimens were available for immunohistochemical staining with a polyclonal anti-CIP2A antibody, together with 19 control nasal mucosa specimens. CIP2A staining intensity and tissue eosinophilia were assessed by two blinded observers with a light microscope. Subject characteristics from 90 patients and 19 controls were obtained from patient records and additionally by a questionnaire from controls. The follow-up data was available from patient records of 84 patients and 16 controls.
  
  
  RESULTS
  The expression of epithelial CIP2A was detected both in control inferior turbinate mucosa and nasal polyps. The expression was significantly lower in the CRSwNP + AERD group compared to controls and CRSwNP without AERD (p < 0.01). High mucosal eosinophilia associated with CRSwNP (p < 0.01). Neither CIP2A nor eosinophilia predicted the need for revision surgery (p > 0.05), whereas previous surgery, allergic rhinitis, and use of corticosteroids did predict the need for revision surgery (p < 0.05).
authors.affiliation: a Haartman Institute, University of Helsinki , Helsinki , Finland.
authors.forename: Emma
authors.initials: E
authors.lastname: Terna
authors.affiliation: a Haartman Institute, University of Helsinki , Helsinki , Finland.
authors.forename: Annika
authors.initials: A
authors.lastname: Luukkainen
authors.affiliation: a Haartman Institute, University of Helsinki , Helsinki , Finland.
authors.forename: Miia
authors.initials: M
authors.lastname: Seppälä
authors.affiliation: a Haartman Institute, University of Helsinki , Helsinki , Finland.|b HUSLAB , Helsinki University Hospital , Helsinki , Finland.
authors.forename: Risto
authors.initials: R
authors.lastname: Renkonen
authors.affiliation: c School of Health Sciences , University of Tampere , Tampere , Finland.
authors.forename: Heini
authors.initials: H
authors.lastname: Huhtala
authors.affiliation: d Fimlab Laboratories Ltd , Tampere , Finland.
authors.forename: Satu
authors.initials: S
authors.lastname: Tommola
authors.affiliation: d Fimlab Laboratories Ltd , Tampere , Finland.|e Department of Pathology , University of Tampere , Tampere , Finland.
authors.forename: Timo
authors.initials: T
authors.lastname: Paavonen
authors.affiliation: f Department of Allergy , University of Helsinki and Helsinki University Hospital , Helsinki , Finland.
authors.forename: Paula
authors.initials: P
authors.lastname: Kauppi
authors.affiliation: b HUSLAB , Helsinki University Hospital , Helsinki , Finland.
authors.forename: Olli
authors.initials: O
authors.lastname: Tynninen
authors.affiliation: b HUSLAB , Helsinki University Hospital , Helsinki , Finland.|g Department of Dermatology , University of Helsinki and Helsinki University Hospital , Helsinki , Finland.
authors.forename: Leila
authors.initials: L
authors.lastname: Jeskanen
authors.affiliation: a Haartman Institute, University of Helsinki , Helsinki , Finland.|f Department of Allergy , University of Helsinki and Helsinki University Hospital , Helsinki , Finland.
authors.forename: Sanna
authors.initials: S
authors.lastname: Toppila-Salmi
mesh_terms: D000328:Adult; D001324:Autoantigens / Q000378:metabolism*; D004802:Eosinophilia; D005260:Female; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D009298:Nasal Polyps / Q000276:immunology / Q000378:metabolism*; D012189:Retrospective Studies; D012220:Rhinitis / Q000276:immunology / Q000378:metabolism*; D012852:Sinusitis / Q000276:immunology / Q000378:metabolism*
chemical_list: D001324:Autoantigens; C500266:CIP2A protein, human; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins
keywords: Aspirin exacerbated respiratory disease; cancerous inhibitor of protein phosphatase 2A; immunohistochemistry; nasal polyp; sinusitis