title: Green tea extract catechin improves internal cardiac muscle relaxation in RCM mice.
abstract: BACKGROUND
  Diastolic dysfunction refers to an impaired relaxation and an abnormality in a heart's filling during diastole while left ventricular systolic function is preserved. Diastolic dysfunction is commonly observed in patients with primary hypertension, diabetes and cardiomyopathies such as hypertrophic cardiomyopathy or restrictive cardiomyopathy. We have generated a restrictive cardiomyopathy (RCM) mouse model with troponin mutations in the heart to mimic the human RCM patients carrying the same mutations.
  
  
  RESULTS
  In the present study, we have investigated the ventricular muscle internal dynamics and pressure developed during systole and diastole by inserting a micro-catheter into the left ventricle of the RCM mice with or without treatment of desensitizer green tea extracts catechins. Our results demonstrate that green tea catechin is able to correct diastolic dysfunction in RCM mainly by improving ventricular compliance and reducing the internal muscle rigidity caused by myofibril hypersensitivity to Ca(2+).
  
  
  CONCLUSIONS
  Green tea extract catechin is effective in correcting diastolic dysfunction and improving ventricular muscle intrinsic compliance in RCM caused by troponin mutations.
authors.affiliation: Department of Pediatric Cardiology, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, 610041, China.|Department of Biomedical Science, Charles E. Schmidt College of Medicine, Florida Atlantic University, 777 Glades Road, Boca Raton, FL, 33431, USA.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Biomedical Science, Charles E. Schmidt College of Medicine, Florida Atlantic University, 777 Glades Road, Boca Raton, FL, 33431, USA.
authors.forename: Zhengyu
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Pediatric Cardiology, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, 610041, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Wu
authors.affiliation: Department of Biomedical Science, Charles E. Schmidt College of Medicine, Florida Atlantic University, 777 Glades Road, Boca Raton, FL, 33431, USA.
authors.forename: Changlong
authors.initials: C
authors.lastname: Nan
authors.affiliation: Department of Biomedical Science, Charles E. Schmidt College of Medicine, Florida Atlantic University, 777 Glades Road, Boca Raton, FL, 33431, USA.
authors.forename: Wen
authors.initials: W
authors.lastname: Shen
authors.affiliation: Department of Pediatric Cardiology, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, 610041, China. Nathan_hua@163.com.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Hua
authors.affiliation: Department of Biomedical Science, Charles E. Schmidt College of Medicine, Florida Atlantic University, 777 Glades Road, Boca Raton, FL, 33431, USA. xhuang@fau.edu.
authors.forename: Xupei
authors.initials: X
authors.lastname: Huang
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects* / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic; D009126:Muscle Relaxation / Q000187:drug effects* / Q000235:genetics; D009206:Myocardium / Q000378:metabolism*; D009210:Myofibrils / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D013662:Tea / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D013662:Tea; D002118:Calcium
keywords: Calcium desensitization; Diastolic dysfunction; Experimental animals; Green tea extracts; Pressure-volume relationship; Restrictive cardiomyopathy