title: Whole genome sequence analysis of an Alachlor and Endosulfan degrading Pseudomonas strain W15Feb9B isolated from Ochlockonee River, Florida.
abstract: We recently isolated a Pseudomonas sp. strain W15Feb9B from Ochlockonee River, Florida and demonstrated potent biodegradative activity against two commonly used pesticides - Alachlor [(2-chloro-2',6'-diethylphenyl-N (methoxymethyl)acetanilide)] and Endosulfan [(6,7,8,9,10,10-hexachloro-1,5,5a,6,9,9a-hexahydro-6,9methano-2,3,4-benzo(e)di-oxathiepin-3-oxide], respectively. To further identify the repertoire of metabolic functions possessed by strain W15Feb9B, a draft genome sequence was obtained, assembled, annotated and analyzed. The genome sequence of strain 2385 has been deposited in GenBank under accession number JTKF00000000; BioSample number SAMN03151543. The sequences obtained from strain 2385 assembled into 192 contigs with a genome size of 6,031,588, G + C content of 60.34, and 5512 total number of putative genes. RAST annotated a total of 542 subsystems in the genome of strain W15Feb9B along with the presence of 5360 coding sequences. A genome wide survey of strain W15Feb9B indicated that it has the potential to degrade several other pollutants including atrazine, caprolactam, dioxin, PAHs (such as naphthalene) and several chloroaromatic compounds.
authors.affiliation: Environmental Biotechnology and Genomics Laboratory, School of the Environment, 1515 S. Martin Luther King Jr. Blvd., Suite 305B, FSH Science Research Center, Florida A&M University, Tallahassee, FL- 32307, USA.
authors.forename: Ashvini
authors.initials: A
authors.lastname: Chauhan
authors.affiliation: Environmental Biotechnology and Genomics Laboratory, School of the Environment, 1515 S. Martin Luther King Jr. Blvd., Suite 305B, FSH Science Research Center, Florida A&M University, Tallahassee, FL- 32307, USA.
authors.forename: Ashish
authors.initials: A
authors.lastname: Pathak
authors.affiliation: Department of Microbiology, Central Laboratory for Environmental Quality Monitoring (CLEQM), National Water Research Center, Egypt.
authors.forename: Ayman Y I
authors.initials: AY
authors.lastname: Ewida
authors.affiliation: Environmental Biotechnology and Genomics Laboratory, School of the Environment, 1515 S. Martin Luther King Jr. Blvd., Suite 305B, FSH Science Research Center, Florida A&M University, Tallahassee, FL- 32307, USA.
authors.forename: Zabrenna
authors.initials: Z
authors.lastname: Griffiths
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB T6G2P5, Canada.
authors.forename: Paul
authors.initials: P
authors.lastname: Stothard
keywords: Alachlor; Biodegradation; Endosulfan; Pseudomonas; Whole Genome Sequencing (WGS)