title: Preparation of osthole-loaded nano-vesicles for skin delivery: Characterization, in vitro skin permeation and preliminary in vivo pharmacokinetic studies.
abstract: The main objective of the present research was to evaluate the flexible nano-vesicles for transdermal delivery of osthole. The nano-vesicles were formulated, characterized and evaluated for their physicochemical properties, in vitro skin permeation and in vivo plasma concentration. The encapsulation efficiency of osthole in ethosome and transfersome was measured to be 83.3±4.8% and 80.9±3.6%, respectively. In vitro studies showed that osthole ethosome provided an enhanced transdermal flux of 6.98±1.6μg/cm(2)/h and a decreased lag time of 2.45h across porcine ear skin. Moreover, ethosome also showed increased skin deposition of the drug over transfersome (1.5-fold) and saturated solution of osthole in 35% ethanol (2.1-fold). Data from in vivo pharmacokinetic studies showed that AUC and Cmax of the osthole loaded-ethosome were remarkably increasing compared with the other formulations. Thus, osthole ethosome was considered as an effective delivery system for the drug.
authors.affiliation: Liaoning Institute of Family Planning, 10 Puhe Street, Shenyang, Liaoning Province, China; Key Laboratory of Reproductive Health and Medical Genetics, National Health and Family Planning Commission, 10 Puhe Street, Shenyang, Liaoning Province, China. Electronic address: mengshu888@sina.com.
authors.forename: Shu
authors.initials: S
authors.lastname: Meng
authors.affiliation: Liaoning Institute of Family Planning, 10 Puhe Street, Shenyang, Liaoning Province, China; Key Laboratory of Reproductive Health and Medical Genetics, National Health and Family Planning Commission, 10 Puhe Street, Shenyang, Liaoning Province, China.
authors.forename: Chong
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Affiliated Hospital of Shandong University of Traditional Chinese Medicine, 6369 Jing Shi Road, Jinan, Shandong Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
authors.affiliation: Liaoning Institute of Family Planning, 10 Puhe Street, Shenyang, Liaoning Province, China; Key Laboratory of Reproductive Health and Medical Genetics, National Health and Family Planning Commission, 10 Puhe Street, Shenyang, Liaoning Province, China.
authors.forename: Xiao-Wei
authors.initials: XW
authors.lastname: Zhang
authors.affiliation: Liaoning Institute of Family Planning, 10 Puhe Street, Shenyang, Liaoning Province, China; Key Laboratory of Reproductive Health and Medical Genetics, National Health and Family Planning Commission, 10 Puhe Street, Shenyang, Liaoning Province, China.
authors.forename: Dan-Hua
authors.initials: DH
authors.lastname: Liu
authors.affiliation: Liaoning Institute of Family Planning, 10 Puhe Street, Shenyang, Liaoning Province, China; Key Laboratory of Reproductive Health and Medical Genetics, National Health and Family Planning Commission, 10 Puhe Street, Shenyang, Liaoning Province, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.affiliation: Liaoning Institute of Family Planning, 10 Puhe Street, Shenyang, Liaoning Province, China; Key Laboratory of Reproductive Health and Medical Genetics, National Health and Family Planning Commission, 10 Puhe Street, Shenyang, Liaoning Province, China.
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Li
authors.affiliation: Liaoning Institute of Family Planning, 10 Puhe Street, Shenyang, Liaoning Province, China; Key Laboratory of Reproductive Health and Medical Genetics, National Health and Family Planning Commission, 10 Puhe Street, Shenyang, Liaoning Province, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Jin
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D003374:Coumarins / Q000008:administration & dosage* / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D016503:Drug Delivery Systems; D065546:Drug Liberation; D049329:Nanostructures / Q000008:administration & dosage* / Q000737:chemistry; D010316:Particle Size; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000378:metabolism; D012869:Skin Absorption; D012995:Solubility; D013499:Surface Properties; D013552:Swine
chemical_list: D001798:Blood Proteins; D003374:Coumarins; C046627:osthol
keywords: Ethosome; Osthole; Pharmacokinetics; Transfersome