title: Protective immunity of grass carp immunized with DNA vaccine against Aeromonas hydrophila by using carbon nanotubes as a carrier molecule.
abstract: To reduce the economic losses caused by diseases in aquaculture industry, more efficient and economic prophylactic measures should be urgently investigated. In this research, the effects of a novel functionalized single-walled carbon nanotubes (SWCNTs) applied as a delivery vehicle for DNA vaccine administration in juvenile grass carp against Aeromonas hydrophila were studied. Our results showed that SWCNTs loaded with DNA vaccine induced a better protection to juvenile grass carp against A. hydrophila. Moreover, SWCNTs conjugated with DNA vaccine provided significantly protective immunity compared with free DNA vaccine. Thereby, SWCNTs may be considered as a potential efficient DNA vaccine carrier to enhance the immunological activity.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Xinong Road 22nd, Yangling, Shaanxi, 712100, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Xinong Road 22nd, Yangling, Shaanxi, 712100, China.
authors.forename: Yu-Xin
authors.initials: YX
authors.lastname: Gong
authors.affiliation: College of Science, Northwest A&F University, Xinong Road 22nd, Yangling, Shaanxi, 712100, China.
authors.forename: Guang-Lu
authors.initials: GL
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Xinong Road 22nd, Yangling, Shaanxi, 712100, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhu
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Xinong Road 22nd, Yangling, Shaanxi, 712100, China. Electronic address: wanggaoxue@126.com.
authors.forename: Gao-Xue
authors.initials: GX
authors.lastname: Wang
mesh_terms: D016980:Aeromonas hydrophila / Q000276:immunology*; D000818:Animals; D001428:Bacterial Vaccines / Q000276:immunology*; D002347:Carps; D005393:Fish Diseases / Q000276:immunology* / Q000382:microbiology; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D007113:Immunity, Innate; D037742:Nanotubes, Carbon; D011897:Random Allocation; D019444:Vaccines, DNA / Q000276:immunology
chemical_list: D001428:Bacterial Vaccines; D037742:Nanotubes, Carbon; D019444:Vaccines, DNA
keywords: Aeromonas hydrophila; DNA vaccine; Immunological activity; Single-walled carbon nanotubes