title: Derivation of Huntington Disease affected Genea017 human embryonic stem cell line.
abstract: The Genea017 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, carrying Htt gene CAG expansion of 40 repeats, indicative of Huntington Disease. Following ICM outgrowth on inactivated human feeders, genetic analysis confirmed a 46, XY karyotype and male allele pattern through CGH and STR analysis. The hESC line had pluripotent cell morphology, 87% of cells expressed Nanog, 95% Oct4, 88% Tra1-60 and 99% SSEA4, gave a PluriTest pluripotency score of 34.74, novelty of 1.27, demonstrated alkaline phosphatase activity and tri-lineage teratoma formation. The cell line was negative for Mycoplasma and visible contamination.
authors.affiliation: Genea Biocells, Sydney, Australia.
authors.forename: Biljana
authors.initials: B
authors.lastname: Dumevska
authors.affiliation: Genea Biocells, Sydney, Australia.
authors.forename: Robert
authors.initials: R
authors.lastname: McKernan
authors.affiliation: Genea Biocells, Sydney, Australia.
authors.forename: Divya
authors.initials: D
authors.lastname: Goel
authors.affiliation: Genea Biocells, Sydney, Australia.
authors.forename: Uli
authors.initials: U
authors.lastname: Schmidt
authors.affiliation: Genea Biocells, Sydney, Australia.
authors.forename: Teija
authors.initials: T
authors.lastname: Peura
mesh_terms: D000483:Alleles; D000818:Animals; D002478:Cells, Cultured; D055028:Comparative Genomic Hybridization; D004622:Embryo, Mammalian / Q000166:cytology*; D005838:Genotype; D006650:Histocompatibility Testing; D000066449:Human Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D006801:Humans; D000071058:Huntingtin Protein / Q000235:genetics; D006816:Huntington Disease / Q000378:metabolism / Q000473:pathology*; D059785:Karyotype; D008297:Male; D051379:Mice; D016513:Mice, SCID; D018895:Microsatellite Repeats / Q000235:genetics; D008856:Microscopy, Fluorescence; D013724:Teratoma / Q000378:metabolism / Q000473:pathology; D014157:Transcription Factors / Q000378:metabolism; D014183:Transplantation, Heterologous
chemical_list: C086055:HTT protein, human; D000071058:Huntingtin Protein; D014157:Transcription Factors