title: Plant Host and Geographic Location Drive Endophyte Community Composition in the Face of Perturbation.
abstract: All plants form symbioses with endophytic fungi, which affect host plant health and function. Most endophytic fungi are horizontally transmitted, and consequently, local environment and geographic location greatly influence endophyte community composition. Growing evidence also suggests that identity of the plant host (e.g., species, genotype) can be important in shaping endophyte communities. However, little is known about how disturbances to plants affect their fungal symbiont communities. The goal of this study was to test if disturbances, from both natural and anthropogenic sources, can alter endophyte communities independent of geographic location or plant host identity. Using the plant species white snakeroot (Ageratina altissima; Asteraceae), we conducted two experiments that tested the effect of perturbation on endophyte communities. First, we examined endophyte response to leaf mining insect activity, a natural perturbation, in three replicate populations. Second, for one population, we applied fungicide to plant leaves to test endophyte community response to an anthropogenic perturbation. Using culture-based methods and Sanger sequencing of fungal isolates, we then examined abundance, diversity, and community structure of endophytic fungi in leaves subjected to perturbations by leaf mining and fungicide application. Our results show that plant host individual and geographic location are the major determinants of endophyte community composition even in the face of perturbations. Unexpectedly, we found that leaf mining did not impact endophyte communities in white snakeroot, but fungicide treatment resulted in small but significant changes in endophyte community structure. Together, our results suggest that endophyte communities are highly resistant to biotic and anthropogenic disturbances.
authors.affiliation: Evolution, Ecology and Behavior Program, Department of Biology, Indiana University, 1001 E. 3rd St., Bloomington, IN, 47405, USA. nschrist@indiana.edu.
authors.forename: Natalie
authors.identifier: 0000-0003-1568-7645
authors.initials: N
authors.lastname: Christian
authors.affiliation: Medical Sciences Program, Indiana University School of Medicine, 1001 E. 3rd St., Bloomington, IN, 47405, USA.
authors.forename: Courtney
authors.initials: C
authors.lastname: Sullivan
authors.affiliation: Evolution, Ecology and Behavior Program, Department of Biology, Indiana University, 1001 E. 3rd St., Bloomington, IN, 47405, USA.
authors.forename: Noelle D
authors.initials: ND
authors.lastname: Visser
authors.affiliation: Evolution, Ecology and Behavior Program, Department of Biology, Indiana University, 1001 E. 3rd St., Bloomington, IN, 47405, USA.
authors.forename: Keith
authors.initials: K
authors.lastname: Clay
mesh_terms: D000818:Animals; D044822:Biodiversity; D060026:Endophytes / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification; D005145:Face / Q000382:microbiology*; D005658:Fungi / Q000145:classification* / Q000187:drug effects / Q000302:isolation & purification; D060434:Herbivory; D007196:Indiana; D007313:Insecta / Q000382:microbiology; D064307:Microbiota / Q000502:physiology; D008906:Mining; D010575:Pesticides; D010802:Phylogeny; D018515:Plant Leaves / Q000382:microbiology; D010944:Plants / Q000145:classification* / Q000382:microbiology*; D012988:Soil Microbiology; D013045:Species Specificity; D013559:Symbiosis
chemical_list: D010575:Pesticides
keywords: Ageratina altissima; Disturbance; Fungi; Fungicide; Herbivory; Microbiome