title: ESE1 is Associated with Neuronal Apoptosis in Lipopolysaccharide Induced Neuroinflammation.
abstract: Neuronal apoptosis induced by the over-activation of microglia during neuroinflammation contributes to the pathology of central nervous system (CNS) degenerative diseases. ESE1 regulates apoptosis of intestinal epithelial cells in ulcerative colitis via accelerating NF-κB activation. NF-κB activation participates in neuronal apoptosis. However, the expression and functions of ESE1 in neuronal apoptosis during CNS inflammatory response remain unclear. In present study, ESE1 expression significantly increased in cerebral cortex after lipopolysaccharide (LPS) intracerebroventricular injection. Immunofluorescence staining indicated that ESE1 was located in neurons. Furthermore, there was a concomitant up-regulation of apoptotic markers including active caspase-3, BAX and decreased expression of anti-apoptosis protein Bcl-2. In vitro, ESE1 depletion in cortical primary neurons inhibited active caspase-3 and BAX expression as well as lactate dehydrogenase (LDH) release with up-regulation of Bcl-2, while ESE1 overexpression can exert opposite effects, indicating that ESE1 promoted neuronal apoptosis induced by LPS or LPS exposed microglia conditioned media (CM). ESE1 accelerated NF-κB activation in neurons with CM treatment. Collectively, all these data suggested that ESE1 might boost neuronal apoptosis during neuroinflammation via up-regulating NF-κB activation. These findings have implications on the potential target of ESE1 in CNS inflammation treatment.
authors.affiliation: School of Clinical Medicine, Anhui Medical University, Wuxi, 214044, Jiangsu Province, China.|Department of Neurosurgery, Army's Traumatic Brain Injury Center, No.101 Hospital of Chinese PLA, Wuxi, 214044, Jiangsu Province, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Jiangsu Province Key Laboratory of Neuroregeneration, Nantong University, Nantong, 226001, Jiangsu Province, China.|Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong, 226001, Jiangsu Province, China.
authors.forename: Huaqing
authors.initials: H
authors.lastname: Xue
authors.affiliation: School of Clinical Medicine, Anhui Medical University, Wuxi, 214044, Jiangsu Province, China.|Department of Neurosurgery, Army's Traumatic Brain Injury Center, No.101 Hospital of Chinese PLA, Wuxi, 214044, Jiangsu Province, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhu
authors.affiliation: School of Clinical Medicine, Anhui Medical University, Wuxi, 214044, Jiangsu Province, China.|Department of Neurosurgery, Army's Traumatic Brain Injury Center, No.101 Hospital of Chinese PLA, Wuxi, 214044, Jiangsu Province, China.
authors.forename: Likun
authors.initials: L
authors.lastname: Yang
authors.affiliation: School of Clinical Medicine, Anhui Medical University, Wuxi, 214044, Jiangsu Province, China.|Department of Neurosurgery, Army's Traumatic Brain Injury Center, No.101 Hospital of Chinese PLA, Wuxi, 214044, Jiangsu Province, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, China.|Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong, 226001, Jiangsu Province, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Qian
authors.affiliation: School of Clinical Medicine, Anhui Medical University, Wuxi, 214044, Jiangsu Province, China.|Department of Neurosurgery, Army's Traumatic Brain Injury Center, No.101 Hospital of Chinese PLA, Wuxi, 214044, Jiangsu Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Lin
authors.affiliation: School of Clinical Medicine, Anhui Medical University, Wuxi, 214044, Jiangsu Province, China. yuhaiwangcn@gmail.com.|Department of Neurosurgery, Army's Traumatic Brain Injury Center, No.101 Hospital of Chinese PLA, Wuxi, 214044, Jiangsu Province, China. yuhaiwangcn@gmail.com.
authors.forename: Yuhai
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D017628:Microglia / Q000187:drug effects*; D016328:NF-kappa B / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects; D014157:Transcription Factors / Q000378:metabolism*; D015533:Transcriptional Activation / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects
chemical_list: C000616615:ELF3 protein, rat; D008070:Lipopolysaccharides; D016328:NF-kappa B; D014157:Transcription Factors
keywords: ESE1; Lipopolysaccharide; Neuroinflammation; Neuronal apoptosis; Rats