title: Regulation of Neurospora crassa cell wall remodeling via the cot-1 pathway is mediated by gul-1.
abstract: Impairment of the Neurospora crassa Nuclear DBF2-related kinase-encoding gene cot-1 results in pleiotropic effects, including abnormally thick hyphal cell walls and septa. An increase in the transcript abundance of genes encoding chitin and glucan synthases and the chitinase gh18-5, but not the cell wall integrity pathway transcription factor rlm-1, accompany the phenotypic changes observed. Deletion of chs-5 or chs-7 in a cot-1 background results in a reduction of hyperbranching frequency characteristic of the cot-1 parent. gul-1 (a homologue of the yeast SSD1 gene) encodes a translational regulator and has been shown to partially suppress cot-1. We demonstrate that the high expression levels of the cell wall remodeling genes analyzed is curbed, and reaches near wild type levels, when gul-1 is inactivated. This is accompanied by morphological changes that include reduced cell wall thickness and restoration of normal chitin levels. We conclude that gul-1 is a mediator of cell wall remodeling within the cot-1 pathway.
authors.affiliation: Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, 7610000, Rehovot, Israel.
authors.forename: Inbal
authors.initials: I
authors.lastname: Herold
authors.affiliation: Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, 7610000, Rehovot, Israel. Oded.Yarden@mail.huji.ac.il.
authors.forename: Oded
authors.identifier: http://orcid.org/0000-0002-4722-535X
authors.initials: O
authors.lastname: Yarden
mesh_terms: D050260:Carbohydrate Metabolism; D002473:Cell Wall / Q000235:genetics* / Q000378:metabolism* / Q000648:ultrastructure; D002688:Chitinases / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D009492:Neurospora crassa / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure; D010641:Phenotype; D017384:Sequence Deletion; D015398:Signal Transduction; D014158:Transcription, Genetic
chemical_list: D005656:Fungal Proteins; D002688:Chitinases
keywords: Cell wall remodeling; Chitin synthase; Ssd1; cot-1; fks-1; gul-1