title: Sperm quality and fertility in rats after prenatal exposure to low doses of TCDD: A three-generation study.
abstract: Exposure to Tetrachlorodibenzo-p-dioxin (TCDD) in male rats promotes, decreased sperm concentration, alterations in motility and in sperm transit time. We evaluated the effect transgenerational of in utero exposure to low doses TCDD in the sperm quality. Pregnant rats (F0) were exposed to 0.1; 0.5 and 1.0μg of TCDD, on gestational day 15, coincides with the end of most organogenesis in the fetus. Adult male offspring (F1, F2 and F3 generation) were investigated for fertility after artificial insemination in utero. After collection of the uterus and ovaries, the numbers of corpora lutea and implants were determined. TCDD provoked alterations in sperm morphology and diminution in serum testosterone levels and sperm transit time in the cauda epididymis. The fertility significantly decreased in all the generations, at least at one dose. In conclusion, TCDD exposure decreases rat sperm quality and fertility in adult male offspring and this effects persist into the next generation.
authors.affiliation: Laboratory of Reproductive and Developmental Biology and Toxicology, Department of Morphology, Institute of Biosciences of Botucatu, UNESP-Univ Estadual Paulista, Botucatu, SP, Brazil. Electronic address: marcisanabria@gmail.com.
authors.forename: Marciana
authors.initials: M
authors.lastname: Sanabria
authors.affiliation: Laboratory of Reproductive and Developmental Biology and Toxicology, Department of Morphology, Institute of Biosciences of Botucatu, UNESP-Univ Estadual Paulista, Botucatu, SP, Brazil.
authors.forename: Maira Smaniotto
authors.initials: MS
authors.lastname: Cucielo
authors.affiliation: Laboratory of Reproductive and Developmental Biology and Toxicology, Department of Morphology, Institute of Biosciences of Botucatu, UNESP-Univ Estadual Paulista, Botucatu, SP, Brazil.
authors.forename: Marina Trevizan
authors.initials: MT
authors.lastname: Guerra
authors.affiliation: Laboratory of Reproductive and Developmental Biology and Toxicology, Department of Morphology, Institute of Biosciences of Botucatu, UNESP-Univ Estadual Paulista, Botucatu, SP, Brazil.
authors.forename: Cibele
authors.initials: C
authors.lastname: Dos Santos Borges
authors.affiliation: Laboratory of Reproductive and Developmental Biology and Toxicology, Department of Morphology, Institute of Biosciences of Botucatu, UNESP-Univ Estadual Paulista, Botucatu, SP, Brazil.
authors.forename: Thais Petrochelli
authors.initials: TP
authors.lastname: Banzato
authors.affiliation: Laboratory of Reproductive and Developmental Biology and Toxicology, Department of Morphology, Institute of Biosciences of Botucatu, UNESP-Univ Estadual Paulista, Botucatu, SP, Brazil.
authors.forename: Juliana Elaine
authors.initials: JE
authors.lastname: Perobelli
authors.affiliation: Laboratory of Reproductive and Developmental Biology and Toxicology, Department of Morphology, Institute of Biosciences of Botucatu, UNESP-Univ Estadual Paulista, Botucatu, SP, Brazil.
authors.forename: Gabriel Adan Araújo
authors.initials: GA
authors.lastname: Leite
authors.affiliation: Department of Morphology, Stomatology and Physiology, School of Dentistry, USP-University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
authors.forename: Janete Aparecida
authors.initials: JA
authors.lastname: Anselmo-Franci
authors.affiliation: Laboratory of Reproductive and Developmental Biology and Toxicology, Department of Morphology, Institute of Biosciences of Botucatu, UNESP-Univ Estadual Paulista, Botucatu, SP, Brazil.
authors.forename: Wilma
authors.initials: W
authors.lastname: De Grava Kempinas
mesh_terms: D000818:Animals; D004785:Environmental Pollutants / Q000633:toxicity*; D005260:Female; D005298:Fertility / Q000187:drug effects*; D008297:Male; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D017208:Rats, Wistar; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology; D013739:Testosterone / Q000097:blood
chemical_list: D004785:Environmental Pollutants; D000072317:Polychlorinated Dibenzodioxins; D013739:Testosterone
keywords: Dioxin; Epididymis; Fertility; Sperm quality; Transgenerational effect