title: The participation of outer membrane proteins in the bacterial sensitivity to nanosilver.
abstract: The presented study is to analyze the participation of outer membrane proteins of Gram- negative bacteria in sensitivity to silver nanomaterials. The mechanism of interaction of silver with the bacterial cell is best described in this group of microorganisms. There are several theories regarding the effectiveness of antimicrobial ions and nanosilver, and at the indicated differences in the way they work. Outer membrane proteins of Gram-negative bacteria are involved in the procurement of silver from the environment and contribute to the development mechanisms of resistance to nanometals. They are measurable parameter in the field of cell phenotypic response to the presence of Gram-negative bacteria in the environment silver nanoforms: its properties, chemical composition, content or times of action. Proteomic methods (including two dimensional electrophoresis and MALDI‑TOF MS) are therefore relevant techniques for determining the susceptibility of bacteria to silver and the changes taking place in the outer membrane under the influence: uptime/exposure and physical and chemical parameters of silver nanomaterials. Many products containing nanosilver is still in the research phase in terms of physico‑chemical characteristics and biological activity, others have been already implemented in many industries. During the very fast nanotechnology developing and introduction to the market products based on the nanosilver the bacterial answer to nanosilver is needed.
authors.affiliation: Zakład Mikrobiologii, Instytut Genetyki i Mikrobiologii, Uniwersytet Wrocławski, Wrocław.
authors.forename: Anna
authors.initials: A
authors.lastname: Kędziora
authors.affiliation: Zakład Mikrobiologii Lekarskiej, Instytut Immunologii i Terapii Doświadczalnej PAN we Wrocławiu.
authors.forename: Eva
authors.initials: E
authors.lastname: Krzyżewska
authors.affiliation: Zakład Mikrobiologii, Instytut Genetyki i Mikrobiologii, Uniwersytet Wrocławski, Wrocław.
authors.forename: Bartłomiej
authors.initials: B
authors.lastname: Dudek
authors.affiliation: Zakład Mikrobiologii, Instytut Genetyki i Mikrobiologii, Uniwersytet Wrocławski, Wrocław.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Bugla-Płoskońska
mesh_terms: D000890:Anti-Infective Agents / Q000627:therapeutic use*; D001424:Bacterial Infections / Q000188:drug therapy*; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D008565:Membrane Proteins / Q000187:drug effects*; D049329:Nanostructures / Q000627:therapeutic use*; D012834:Silver / Q000627:therapeutic use*
chemical_list: D000890:Anti-Infective Agents; D008565:Membrane Proteins; D012834:Silver