title: Pathogenicity of reassortant H9 influenza viruses with different NA genes in mice and chickens.
abstract: To better understand the influence of different NA genes on pathogenicity of H9 viruses, three reassortant H9 viruses (rH9N1, H9N2 and rH9N3) were generated and characterized. All three viruses replicated efficiently in eggs and MDCK cells, whereas the rH9N1 and rH9N3 replicated more efficiently than H9N2 in A549 cells. The rH9N3 replicated more efficiently than rH9N1 and H9N2 viruses in mice, however, rH9N3 replicated and shed less efficiently than the H9N2 virus in chickens. Further studies indicate that N3 had higher NA activity and released virus from erythrocytes faster, which may improve the adaptation of H9 influenza virus to mammals.
authors.affiliation: Chinese Academy of Agricultural Sciences, Shanghai Veterinary Research Institute, Shanghai, China.|College of Veterinary Medicine, Nanjing Agriculture University, Nanjing, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Yan
authors.affiliation: Chinese Academy of Agricultural Sciences, Shanghai Veterinary Research Institute, Shanghai, China.
authors.forename: Qinfang
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Chinese Academy of Agricultural Sciences, Shanghai Veterinary Research Institute, Shanghai, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Su
authors.affiliation: Chinese Academy of Agricultural Sciences, Shanghai Veterinary Research Institute, Shanghai, China.
authors.forename: Qiaoyang
authors.initials: Q
authors.lastname: Teng
authors.affiliation: College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, China.
authors.forename: Danqi
authors.initials: D
authors.lastname: Bao
authors.affiliation: Chinese Academy of Agricultural Sciences, Shanghai Veterinary Research Institute, Shanghai, China.
authors.forename: Guangsheng
authors.initials: G
authors.lastname: Che
authors.affiliation: Chinese Academy of Agricultural Sciences, Shanghai Veterinary Research Institute, Shanghai, China.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Chen
authors.affiliation: Chinese Academy of Agricultural Sciences, Shanghai Veterinary Research Institute, Shanghai, China.
authors.forename: Hongrui
authors.initials: H
authors.lastname: Cui
authors.affiliation: Chinese Academy of Agricultural Sciences, Shanghai Veterinary Research Institute, Shanghai, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Ruan
authors.affiliation: Chinese Academy of Agricultural Sciences, Shanghai Veterinary Research Institute, Shanghai, China.
authors.forename: Xuesong
authors.initials: X
authors.lastname: Li
authors.affiliation: Chinese Academy of Agricultural Sciences, Shanghai Veterinary Research Institute, Shanghai, China. lizejun@shvri.ac.cn.
authors.forename: Zejun
authors.initials: Z
authors.lastname: Li
mesh_terms: D000072283:A549 Cells; D000818:Animals; D002645:Chickens / Q000821:virology; D004285:Dogs; D005814:Genes, Viral / Q000235:genetics / Q000502:physiology; D053127:Influenza A Virus, H9N2 Subtype / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D009980:Influenza A virus / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology; D005585:Influenza in Birds / Q000821:virology*; D061985:Madin Darby Canine Kidney Cells; D051379:Mice / Q000821:virology; D008807:Mice, Inbred BALB C; D009439:Neuraminidase / Q000235:genetics*; D009976:Orthomyxoviridae Infections / Q000821:virology*; D014779:Virus Replication
chemical_list: D009439:Neuraminidase