title: Characteristics and bioactivities of different molecular weight polysaccharides from camellia seed cake.
abstract: Four polysaccharides, namely COP-1, COP-2, COP-3 and COP-4, were ultrafiltrated from crud Camellia oleifera seed cake polysaccharides (COP-c), purified, and characterized, including the determination of antioxidant and antiproliferative activities. Their molecular weights were 7.9, 36, 83 and 225kDa, respectively. All COPs showed the similar FT-IR spectrums, but significant differentials in monosaccharide components. COP-2 exhibited the highest radical scavenging abilities. COP-1 has the strongest metal chelating capabilities. Although with higher molecular weight, COP-4 showed the poorest antioxidant abilities. These results suggested appreciate molecular weight COP possessed a better antioxidant activities. Additionally, all COPs had non-significant antiproliferative abilities in HaLa and HepG2 cells.
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an, 625014, PR China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Xu
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an, 625014, PR China.
authors.forename: Xu
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an, 625014, PR China.
authors.forename: Shiling
authors.initials: S
authors.lastname: Feng
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an, 625014, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an, 625014, PR China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Zhou
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an, 625014, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Yuan
authors.affiliation: College of Life Science, Sichuan Agricultural University, Ya'an, 625014, PR China. Electronic address: dcb@sicau.edu.cn.
authors.forename: Chunbang
authors.initials: C
authors.lastname: Ding
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000737:chemistry; D028244:Camellia / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D002614:Chelating Agents / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000494:pharmacology; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D017665:Hydroxyl Radical / Q000737:chemistry; D020128:Inhibitory Concentration 50; D008670:Metals / Q000494:pharmacology; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D010084:Oxidation-Reduction; D010851:Picrates / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D014462:Ultrafiltration
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D002614:Chelating Agents; D016166:Free Radical Scavengers; D008670:Metals; D009005:Monosaccharides; D010851:Picrates; D011134:Polysaccharides; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Bioactivity; Camellia oleifera; Molecular weight; Polysaccharides; Ultrafiltration