title: [HPLC Fingerprints of Nerviliae fordii and Nervilia plicata].
abstract: OBJECTIVE
  To establish the HPLC fingerprint of the whole plant of Nerviliae fordii and Nervilia plicata, in order to provide a method for evaluation of authenticity and quality control of the whole plant of Nerviliae fordii.
  
  
  METHODS
  15 batches of Nerviliae fordii and 5 batches of Nervilia plicata were analyzed on a Kromasil C18 column (250 mm x 4.6 mm, 5 μm) at room temperature with gradient elution using acetonitrile and 0.2% phosphoric acid as the mobile phase at a flow rate of 1.0 mL/min. The wavelength of detector was 256 nm.
  
  
  RESULTS
  Significant differences were found between Nerviliae fordii and Nervilia plicata. Furthermore, there were no obvious differences observed between Nerviliae fordii of big-leaf and Nerviliae fordii of small-leaf.
  
  
  CONCLUSIONS
  The present-developed HPLC fingerprints method provides a rapid, effective and valuable benchmark for distinguishing of Nerviliae fordii and Nervilia plicata, which is favorable to improve overall quality control of Nerviliae Fordii.
authors.forename: Zhu-jie
authors.initials: ZJ
authors.lastname: Liu
authors.forename: Yin-ping
authors.initials: YP
authors.lastname: Cheng
authors.forename: Chao-zhan
authors.initials: CZ
authors.lastname: Lin
authors.forename: Chen-chen
authors.initials: CC
authors.lastname: Zhu
mesh_terms: D002851:Chromatography, High Pressure Liquid; D029595:Orchidaceae / Q000737:chemistry*; D064209:Phytochemicals / Q000032:analysis*; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D064209:Phytochemicals