title: [Effects of Browning Inhibitors on Suspension Cells Growth and Secondary Metabolites Production in Changium smyrnioides].
abstract: OBJECTIVE
  To study the effects of browning inhibitors on Changium smyrnioides suspension cells growth and secondary metabolites production.
  
  
  METHODS
  Different concentrations of V(C), AC, AHC, Na2S2O3 and PVP were added to the light brown suspension cells, and the contents of phenols, total coumarins, bergaptol and bergapten were determined by UV-Vis and HPLC.
  
  
  RESULTS
  PVP with low concentration and V(C) improved the growth of the suspension cells in different degrees. It was showed that the content of phenols in the suspension cells was related to the kinds of browning inhibitors. The addition of V(C) in the medium increased the content of total coumarins significantly. After using 2 mg/mL of V(C), the gross increase rate of total coumarins was 51.53%, which was 4.8 times than that of the control group. The browning phenomenon caused by salicylic acid were inhibited by adding 2 mg/mL of V(C) into suspension culture system (with salicylic acid as the inducer). At the same time, the content of bergaptol and bergapten was increased 25.96% and 33.33%, respectively.
  
  
  CONCLUSIONS
  V(C) is the best anti-browning agent in this study. It can inhibit browning, and promote cell growth and accumulation of secondary metabolites in Changium smyrnioides suspension cells.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhou
authors.forename: Chen-qiong
authors.initials: CQ
authors.lastname: Xie
authors.forename: Jian-wei
authors.initials: JW
authors.lastname: Chen
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
mesh_terms: D000078223:5-Methoxypsoralen; D019661:Apiaceae / Q000737:chemistry / Q000166:cytology / Q000254:growth & development*; D018929:Cell Culture Techniques; D002851:Chromatography, High Pressure Liquid; D003374:Coumarins / Q000032:analysis*; D003470:Culture Media / Q000737:chemistry*; D011564:Furocoumarins / Q000032:analysis; D008730:Methoxsalen / Q000031:analogs & derivatives / Q000032:analysis; D010636:Phenols / Q000032:analysis; D020156:Salicylic Acid
chemical_list: D003374:Coumarins; D003470:Culture Media; D011564:Furocoumarins; D010636:Phenols; D000078223:5-Methoxypsoralen; C080771:bergaptol; D020156:Salicylic Acid; D008730:Methoxsalen