title: Effects of Yeast and Bacterial Commensals and Pathogens of the Female Genital Tract on the Transepithelial Electrical Resistance of HeLa Cells.
abstract: Commensals of the human body can shift to a pathogenic phase when the host immune system is impaired. This study aims to investigate the effect of seven yeast and two bacterial commensals and opportunistic pathogens isolated from blood and the female genital tract on the transepithelial electrical resistance (TER) of human cervical epithelial cell cultures (HeLa). The pathogens Candida tropicalis, C. parapsilosis, C. glabrata, C. krusei, C. albicans and Saccharomyces cerevisiae, caused a significant decrease in TER as compared to the controls; Lactobacillus spp caused a significant increase in TER versus the controls and Escherichia coli had no effect on the TER of the cell monolayers. The above data show that Candida spp., S. cerevisiae and Lactobacillus spp. have a non-selective effect on the TER of HeLa cell monolayers. These results are consistent with the in vivo non-selective action of these microorganisms on the various human mucosal epithelia.
authors.affiliation: Laboratory of Experimental Neurophysiology, Department of Neurology, Aeginition Hospital, Medical School, National and Kapodistrian University of Athens, Greece.
authors.forename: Vassiliki
authors.initials: V
authors.lastname: Tsata
authors.affiliation: Laboratory of Experimental Neurophysiology, Department of Neurology, Aeginition Hospital, Medical School, National and Kapodistrian University of Athens, Greece.
authors.forename: Aristea
authors.initials: A
authors.lastname: Velegraki
authors.affiliation: Mycology Research Laboratory, Microbiology Department, Medical School, National and Kapodistrian University of Athens, Greece.
authors.forename: Anastasios
authors.initials: A
authors.lastname: Ioannidis
authors.affiliation: Department of Nursing, Faculty of Human Movement and Quality of Life Sciences, University of Peloponnese, Sparta, Greece.
authors.forename: Cornelia
authors.initials: C
authors.lastname: Poulopoulou
authors.affiliation: Department of Computer Science and Biomedical Informatics, University of Thessaly, Greece.
authors.forename: Pantelis
authors.initials: P
authors.lastname: Bagos
authors.affiliation: Department of Biopathology and Clinical Microbiology, Aeginition Hospital, Medical School, National and Kapodistrian University of Athens, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Magana
authors.affiliation: Department of Biopathology and Clinical Microbiology, Aeginition Hospital, Medical School, National and Kapodistrian University of Athens, Greece.
authors.forename: Stylianos
authors.initials: S
authors.lastname: Chatzipanagiotou
keywords: Candida; E. coli; HeLa cells; Lactobacillus; Saccharomyces; Transepithelial electrical resistance