title: Good laboratory practices guarantee biosafety in the Sierra Leone-China friendship biosafety laboratory.
abstract: BACKGROUND
  The outbreak of Ebola virus disease (EVD) in West Africa between 2014 and 2015 was the largest EDV epidemic since the identification of Ebola virus (EBOV) in 1976, and the countries most strongly affected were Sierra Leone, Guinea, and Liberia.
  
  
  RESULTS
  The Sierra Leone-China Friendship Biological Safety Laboratory (SLE-CHN Biosafety Lab), a fixed Biosafety Level 3 laboratory in the capital city of Sierra Leone, was established by the Chinese government and has been active in EBOV detection since 11 March 2015. Complete management and program documents were created for the SLE-CHN Biosafety Lab, and it was divided into four zones (the green, yellow, brown, and red zones) based on the risk assessment. Different types of safe and appropriate personnel protection equipment (PPE) are used in different zones of the laboratory, and it fully meets the Biosafety Level 3 laboratory standards of the World Health Organization.
  
  
  CONCLUSIONS
  Good preparedness, comprehensive risk assessment and operation documents, appropriate PPE, effective monitoring and intensive training, together with well-designed and reasonable laboratory sectioning are essential for guaranteeing biosafety.
authors.affiliation: Chinese Center for Disease Control and Prevention, National Institute for Viral Diseases Control and Prevention, Beijing, China.|Sierra Leone-China Friendship Biological Safety Laboratory, Freetown, Sierra-Leone.|Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Chinese Center for Disease Control and Prevention, National Institute for Viral Diseases Control and Prevention, Beijing, China.|Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing, China.
authors.forename: Wei-Min
authors.initials: WM
authors.lastname: Zhou
authors.affiliation: Chinese Center for Disease Control and Prevention, National Institute for Viral Diseases Control and Prevention, Beijing, China.|Sierra Leone-China Friendship Biological Safety Laboratory, Freetown, Sierra-Leone.|Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Chinese Center for Disease Control and Prevention, National Institute for Viral Diseases Control and Prevention, Beijing, China.|Sierra Leone-China Friendship Biological Safety Laboratory, Freetown, Sierra-Leone.|State Key Laboratory for Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing, China.
authors.forename: Huan-Yu
authors.initials: HY
authors.lastname: Wang
authors.affiliation: Chinese Center for Disease Control and Prevention, National Institute for Viral Diseases Control and Prevention, Beijing, China.|Sierra Leone-China Friendship Biological Safety Laboratory, Freetown, Sierra-Leone.|State Key Laboratory for Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing, China.
authors.forename: Hai-Jun
authors.initials: HJ
authors.lastname: Du
authors.affiliation: Chinese Center for Disease Control and Prevention, National Institute for Viral Diseases Control and Prevention, Beijing, China.|Sierra Leone-China Friendship Biological Safety Laboratory, Freetown, Sierra-Leone.|Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Nie
authors.affiliation: Chinese Center for Disease Control and Prevention, National Institute for Viral Diseases Control and Prevention, Beijing, China.|Sierra Leone-China Friendship Biological Safety Laboratory, Freetown, Sierra-Leone.|State Key Laboratory for Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing, China.
authors.forename: Jing-Dong
authors.initials: JD
authors.lastname: Song
authors.affiliation: Chinese Center for Disease Control and Prevention, National Institute for Viral Diseases Control and Prevention, Beijing, China.|Sierra Leone-China Friendship Biological Safety Laboratory, Freetown, Sierra-Leone.|State Key Laboratory for Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing, China.
authors.forename: Kang
authors.initials: K
authors.lastname: Xiao
authors.affiliation: Chinese Center for Disease Control and Prevention, National Institute for Viral Diseases Control and Prevention, Beijing, China.|Sierra Leone-China Friendship Biological Safety Laboratory, Freetown, Sierra-Leone.|State Key Laboratory for Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing, China.
authors.forename: Wen-Wen
authors.initials: WW
authors.lastname: Lei
authors.affiliation: Chinese Center for Disease Control and Prevention, National Institute for Viral Diseases Control and Prevention, Beijing, China.|Sierra Leone-China Friendship Biological Safety Laboratory, Freetown, Sierra-Leone.|Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing, China.
authors.forename: Jian-Qiang
authors.initials: JQ
authors.lastname: Guo
authors.affiliation: Chinese Center for Disease Control and Prevention, National Institute for Viral Diseases Control and Prevention, Beijing, China.|Sierra Leone-China Friendship Biological Safety Laboratory, Freetown, Sierra-Leone.|Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing, China.
authors.forename: He-Jiang
authors.initials: HJ
authors.lastname: Wei
authors.affiliation: Chinese Center for Disease Control and Prevention, National Institute for Viral Diseases Control and Prevention, Beijing, China.|Sierra Leone-China Friendship Biological Safety Laboratory, Freetown, Sierra-Leone.|Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Cai
authors.affiliation: Chinese Center for Disease Control and Prevention, National Institute for Viral Diseases Control and Prevention, Beijing, China.|Sierra Leone-China Friendship Biological Safety Laboratory, Freetown, Sierra-Leone.|State Key Laboratory for Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing, China.
authors.forename: Yan-Hai
authors.initials: YH
authors.lastname: Wang
authors.affiliation: Chinese Center for Disease Control and Prevention, National Institute for Viral Diseases Control and Prevention, Beijing, China.|Sierra Leone-China Friendship Biological Safety Laboratory, Freetown, Sierra-Leone.
authors.forename: Jiang
authors.initials: J
authors.lastname: Wu
authors.affiliation: Sierra Leone-China Friendship Biological Safety Laboratory, Freetown, Sierra-Leone.
authors.forename: Gerard
authors.initials: G
authors.lastname: Kamara
authors.affiliation: Sierra Leone-China Friendship Biological Safety Laboratory, Freetown, Sierra-Leone.
authors.forename: Idrissa
authors.initials: I
authors.lastname: Kamara
authors.affiliation: Sierra Leone-China Friendship Biological Safety Laboratory, Freetown, Sierra-Leone.|Chinese Center for Disease Control and Prevention, Beijing, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wei
authors.affiliation: Chinese Center for Disease Control and Prevention, National Institute for Viral Diseases Control and Prevention, Beijing, China.|Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing, China.|State Key Laboratory for Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing, China.
authors.forename: Mi-Fang
authors.initials: MF
authors.lastname: Liang
authors.affiliation: Chinese Center for Disease Control and Prevention, National Institute for Viral Diseases Control and Prevention, Beijing, China. wgzcdc@hotmail.com.|Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing, China. wgzcdc@hotmail.com.
authors.forename: Gui-Zhen
authors.initials: GZ
authors.lastname: Wu
authors.affiliation: Chinese Center for Disease Control and Prevention, National Institute for Viral Diseases Control and Prevention, Beijing, China. dongxp238@sina.com.|Sierra Leone-China Friendship Biological Safety Laboratory, Freetown, Sierra-Leone. dongxp238@sina.com.|State Key Laboratory for Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing, China. dongxp238@sina.com.|Chinese Center for Disease Control and Prevention, Beijing, China. dongxp238@sina.com.|Collaborative Innovation Center Diagnosis and Treatment of Infectious Diseases, Zhejiang University, Hangzhou, China. dongxp238@sina.com.|Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China. dongxp238@sina.com.
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Dong
mesh_terms: D029043:Ebolavirus / Q000502:physiology*; D019142:Hemorrhagic Fever, Ebola / Q000821:virology*; D007753:Laboratories / Q000592:standards*; D012449:Safety / Q000592:standards*; D012807:Sierra Leone
keywords: BSL-3 laboratory; Biosafety; Ebola virus; Ebola virus disease; SLE-CHN Biosafety Lab