title: Diagnostic Accuracy of PIK3CA Mutation Detection by Circulating Free DNA in Breast Cancer: A Meta-Analysis of Diagnostic Test Accuracy.
abstract: Mutation of p110 alpha-catalytic subunit of phosphatidylinositol 3-kinase (PIK3CA) has high predictive and prognostic values for breast cancer. Hence, there has been a marked interest in detecting and monitoring PIK3CA genotype with non-invasive technique, such as circulating free DNA (cfDNA). However, the diagnostic accuracy of PIK3CA genotyping by cfDNA is still a problem of controversy. Here, we conducted the first meta-analysis to evaluate overall diagnostic performance of cfDNA for PIK3CA mutation detection. Literature search was performed in Pubmed, Embase and Cochrane Central Register of Controlled Trials databases. Seven cohorts from five studies with 247 patients were included. The pooled sensitivity, specificity, positive and negative likelihood ratio, diagnostic odds ratio and area under summary receiver operating characteristic curve were calculated for accuracy evaluation. The pooled sensitivity and specificity were 0.86 (95% confidence interval [CI] 0.32-0.99) and 0.98 (95% CI 0.86-1.00), respectively; the pooled positive and negative likelihood ratio were 42.8 (95% CI 5.1-356.9) and 0.14 (95% CI 0.02-1.34), respectively; diagnostic odds ratio for evaluating the overall diagnostic performance was 300 (95% CI 8-11867); area under summary receiver operating characteristic curve reached 0.99 (95% CI 0.97-0.99). Subgroup analysis with metastatic breast cancer revealed remarkable improvement in diagnostic performance (sensitivity: 0.86-0.91; specificity: 0.98; diagnostic odds ratio: 300-428). This meta-analysis proved that detecting PIK3CA gene mutation by cfDNA has high diagnostic accuracy in breast cancer, especially for metastatic breast cancer. It may serve as a reliable non-invasive assay for detecting and monitoring PIK3CA mutation status in order to deliver personalized and precise treatment.
authors.affiliation: Department of Breast Surgery, Peking Union Medical College Hospital, Beijing, China, 100730.
authors.forename: Yidong
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Breast Surgery, Peking Union Medical College Hospital, Beijing, China, 100730.
authors.forename: Changjun
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Dermatology, University of California, San Francisco, California, United States of America, 94143-0989.
authors.forename: Hanjiang
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Breast Surgery, Peking Union Medical College Hospital, Beijing, China, 100730.
authors.forename: Yan
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Department of Breast Surgery, Peking Union Medical College Hospital, Beijing, China, 100730.
authors.forename: Bo
authors.initials: B
authors.lastname: Pan
authors.affiliation: Department of Breast Surgery, Peking Union Medical College Hospital, Beijing, China, 100730.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Breast Surgery, Peking Union Medical College Hospital, Beijing, China, 100730.
authors.forename: Xin
authors.initials: X
authors.lastname: Huang
authors.affiliation: Department of Breast Surgery, Peking Union Medical College Hospital, Beijing, China, 100730.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Xu
authors.affiliation: Department of Breast Surgery, Peking Union Medical College Hospital, Beijing, China, 100730.
authors.forename: Yali
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Breast Surgery, Peking Union Medical College Hospital, Beijing, China, 100730.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Sun
mesh_terms: D014408:Biomarkers, Tumor; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000235:genetics*; D058534:Class I Phosphatidylinositol 3-Kinases; D004252:DNA Mutational Analysis / Q000379:methods / Q000592:standards; D004273:DNA, Neoplasm / Q000097:blood / Q000235:genetics*; D030561:Databases, Nucleic Acid; D005260:Female; D018740:Genetic Heterogeneity; D006801:Humans; D009154:Mutation; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics*; D012372:ROC Curve; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D019869:Phosphatidylinositol 3-Kinases; D058534:Class I Phosphatidylinositol 3-Kinases; C484760:PIK3CA protein, human