title: Mg(2+)/Ca(2+) promotes the adhesion of marine bacteria and algae and enhances following biofilm formation in artificial seawater.
abstract: Adhesion of microorganisms in the marine environment is essential for initiation and following development of biofouling. A variety of factors play roles in regulating the adhesion. Here we report the influence of Ca(2+) and Mg(2+) in artificial seawater on attachment and colonization of Bacillus sp., Chlorella and Phaeodactylum tricornutum on silicon wafer. Extra addition of the typical divalent cations in culturing solution gives rise to significantly enhanced adhesion of the microorganisms. Mg(2+) and Ca(2+) affect the adhesion of Bacillus sp. presumably by regulating aggregation and formation of extracellular polymeric substances (EPS). The ions alter quantity and types of the proteins in EPS, in turn affecting subsequent adhesion. However, it is noted that Mg(2+) promotes adhesion of Chlorella likely by regulating EPS formation and polysaccharide synthesis. Ca(2+) plays an important role in protein expression to enhance the adhesion of Chlorella. For Phaeodactylum tricornutum, Ca(2+) expedites protein synthesis for enhanced adhesion. The results shed some light on effective ways of utilizing divalent cations to mediate formation of biofilms on the marine structures for desired performances.
authors.affiliation: Key Laboratory of Marine Materials and Related Technologies, Key Laboratory of Marine Materials and Protective Technologies of Zhejiang Province, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: He
authors.affiliation: Key Laboratory of Marine Materials and Related Technologies, Key Laboratory of Marine Materials and Protective Technologies of Zhejiang Province, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, China.
authors.forename: Jinpeng
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Marine Materials and Related Technologies, Key Laboratory of Marine Materials and Protective Technologies of Zhejiang Province, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Leila
authors.initials: L
authors.lastname: Abdoli
authors.affiliation: Key Laboratory of Marine Materials and Related Technologies, Key Laboratory of Marine Materials and Protective Technologies of Zhejiang Province, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, China. Electronic address: lihua@nimte.ac.cn.
authors.forename: Hua
authors.initials: H
authors.lastname: Li
mesh_terms: D001422:Bacterial Adhesion / Q000187:drug effects*; D001426:Bacterial Proteins / Q000096:biosynthesis; D018441:Biofilms / Q000254:growth & development*; D002118:Calcium / Q000494:pharmacology*; D002708:Chlorella / Q000187:drug effects / Q000502:physiology*; D017377:Diatoms / Q000187:drug effects / Q000502:physiology*; D008274:Magnesium / Q000494:pharmacology*; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D011134:Polysaccharides / Q000138:chemical synthesis; D012623:Seawater / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D011108:Polymers; D011134:Polysaccharides; D008274:Magnesium; D002118:Calcium
keywords: Adhesion; Biofilm; Divalent cations; Polysaccharide synthesis; Protein expression