title: Chemical Coding of Sensory Neurons Supplying the Hip Joint Capsule in the Sheep.
abstract: Immunohistochemical properties of nerve fibres supplying the joint capsule were previously described in many mammalian species, but the localization of sensory neurons supplying this structure was studied only in laboratory animals, the rat and rabbit. However, there is no comprehensive data on the chemical coding of sensory neurons projecting to the hip joint capsule (HJC). The aim of this study was to establish immunohistochemical properties of sensory neurons supplying HJC in the sheep. The study was carried out on 10 sheep, weighing about 30-40 kg. The animals were injected with a retrograde neural tracer Fast Blue (FB) into HJC. Sections of the spinal ganglia (SpG) with FB-positive (FB+) neurons were stained using antibodies against calcitonin gene-related peptide (CGRP) substance P (SP), pituitary adenylate cyclase-activating peptide (PACAP), nitric oxide synthase (n-NOS), neuropeptide Y (NPY), vasoactive intestinal polypeptide (VIP), Leu-5-enkephalin (Leu-Enk), galanin (GAL) and vesicular acetylcholine transporter (VACHT). The vast majority of FB+ neurons supplying HJC was found in the ganglia from the 5th lumbar to the 2nd sacral. Immunohistochemistry revealed that most of these neurons were immunoreactive to CGRP or SP (80.7 ± 8.0% or 56.4 ± 4.8%, respectively) and many of them stained for PACAP or GAL (52.9 ± 2.9% or 50.6 ± 19.7%, respectively). Other populations of FB+ neurons were those immunoreactive to n-NOS (37.8 ± 9.7%), NPY (34.6 ± 6.7%), VIP (28.7 ± 4.8%), Leu-Enk (27.1 ± 14.6) and VACHT (16.7 ± 9.6).
authors.affiliation: Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-719, Olsztyn, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Dudek
authors.affiliation: Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-719, Olsztyn, Poland.
authors.forename: W
authors.initials: W
authors.lastname: Sienkiewicz
authors.affiliation: Department of Animal Anatomy, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Kozuchowska 1/3, 51-631, Wrocław, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Chrószcz
authors.affiliation: Department of Animal Anatomy, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Kozuchowska 1/3, 51-631, Wrocław, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Janeczek
authors.affiliation: Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-719, Olsztyn, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Kaleczyc
mesh_terms: D000578:Amidines; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000276:immunology; D005727:Ganglia, Spinal / Q000276:immunology / Q000502:physiology*; D006621:Hip Joint / Q000294:innervation*; D007150:Immunohistochemistry; D017746:Joint Capsule / Q000294:innervation*; D009478:Neuropeptide Y / Q000276:immunology; D019001:Nitric Oxide Synthase / Q000276:immunology; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide / Q000276:immunology; D011984:Sensory Receptor Cells / Q000276:immunology / Q000502:physiology*; D012756:Sheep; D013194:Staining and Labeling / Q000379:methods*; D013373:Substance P / Q000276:immunology; D014660:Vasoactive Intestinal Peptide / Q000276:immunology; D050494:Vesicular Acetylcholine Transport Proteins / Q000276:immunology
chemical_list: D000578:Amidines; D009478:Neuropeptide Y; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D050494:Vesicular Acetylcholine Transport Proteins; C031455:diamidino compound 253-50; D013373:Substance P; D014660:Vasoactive Intestinal Peptide; D019001:Nitric Oxide Synthase; D015740:Calcitonin Gene-Related Peptide