title: [Diagnostic Values of IL-12 and IFN-γ for the Patients with Acute Leukemia].
abstract: OBJECTIVE
  To explore the diagnostic values of interleukin-12 (IL-12) and interferon-γ (IFN-γ) for the patients with acute leukemia (AL).
  
  
  METHODS
  A total of 76 cases of AL were enrolled in this study, and the 40 healthy persons were used as control group. The levels of IL-12 and IFN-γ were determined by enzyme linked immunosorbent assay (ELISA). The results were analyzed.
  
  
  RESULTS
  The levels of IL-12 and IFN-γ in the untreated AL group, ALL and ANLL groups were lower significantly than those in the control group (P<0.05), there was no significant difference between untreated AL and ANLL groups (P>0.05). The levels of IL-12 and IFN-γ in CR patients of AL group after treatment obviously higher than that of patients before treatment (P<0.05), but there was no significant difference as campared with that in control. The levels of IL-12 and IFN-γ in NR patients of AL group after treatment were obviously lower than that in control group (P<0.05), but there was no significant difference in comparision with patients before treatment (P>0.05). The levels of IL-12 and IFN-γ of AL-CR and AL-NR patients before treatment were not significant difference before treatment (P>0.05). The levels of IL-12 and IFN-γ of AL-CR patients obviously higher than that in AL-NR patients (P<0.05). According to immure classification, the levels of IL-12 and IFN-γ of patients in untreated group were not significant difference. In regard to the clinical risk degree, the level of IL-12 of patients in untreated group was not obvious difference (P>0.05), but the level of IFN-γ of patients in untreated group was obvious different (P<0.05). The level of IL-12 of patients in untreated group positively correlated with level of IFN-γ (r=0.735, P<0.05), but the level of IL-12 did not significantly correlated with the level of IFN-γ (r=0.292, P>0.05).
  
  
  CONCLUSIONS
  The serum levels of both IL-12 and IFN-γ are lower, but the changes of both serum levels may be helpful to diagnose and treatment of AL patients.
authors.affiliation: Department of Clinical Laboratorial Examination, Chengde Municipal Central Hospital, Chengde 067000, Hebei Province, China.
authors.forename: Xiao-Cheng
authors.initials: XC
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratorial Examination, Chengde Municipal Central Hospital, Chengde 067000, Hebei Province, China. E-mail: 565310855@qq.com.
authors.forename: Ze-Jun
authors.initials: ZJ
authors.lastname: Yan
authors.affiliation: Department of Clinical Laboratorial Examination, Chengde Municipal Central Hospital, Chengde 067000, Hebei Province, China.
authors.forename: Jing-Hua
authors.initials: JH
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratorial Examination, Chengde Municipal Central Hospital, Chengde 067000, Hebei Province, China.
authors.forename: Ya-Min
authors.initials: YM
authors.lastname: Fan
authors.affiliation: Department of Clinical Laboratorial Examination, Chengde Municipal Central Hospital, Chengde 067000, Hebei Province, China.
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Xu
mesh_terms: D000208:Acute Disease; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007371:Interferon-gamma / Q000097:blood*; D018664:Interleukin-12 / Q000097:blood*; D015847:Interleukin-4 / Q000097:blood; D007938:Leukemia / Q000097:blood* / Q000175:diagnosis; D012074:Remission Induction
chemical_list: C573415:IFNG protein, human; C508598:IL4 protein, human; D018664:Interleukin-12; D015847:Interleukin-4; D007371:Interferon-gamma