title: Protection efficacy of the Brucella abortus ghost vaccine candidate lysed by the N-terminal 24-amino acid fragment (GI24) of the 36-amino acid peptide PMAP-36 (porcine myeloid antimicrobial peptide 36) in murine models.
abstract: Brucella abortus cells were lysed by the N-terminal 24-amino acid fragment (GI24) of the 36-amino acid peptide PMAP-36 (porcine myeloid antimicrobial peptide 36). Next, the protection efficacy of the lysed fragment as a vaccine candidate was evaluated. Group A mice were immunized with sterile PBS, group B mice were intraperitoneally (ip) immunized with 3 × 108colony-forming units (CFUs) of B. abortus strain RB51, group C mice were immunized ip with 3 × 108cells of the B. abortus vaccine candidate, and group D mice were orally immunized with 3 × 109cells of the B. abortus vaccine candidate. Brucella lipopolysaccharide (LPS)-specific serum IgG titers were considerably higher in groups C and D than in group A. The levels of interleukin (IL)-4, IL-10, tumor necrosis factor alpha (TNF-α) and interferon gamma (IFN-γ) were significantly higher in groups B-D than in group A. After an ip challenge with B. abortus 544, only group C mice showed a significant level of protection as compared to group A. Overall, these results show that ip immunization with a vaccine candidate lysed by GI24 can effectively protect mice from systemic infection with virulent B. abortus.
authors.affiliation: Veterinary Public Health, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University, Iksan 54596, Republic of Korea.
authors.forename: Ae Jeong
authors.initials: AJ
authors.lastname: Kwon
authors.forename: Ja Young
authors.initials: JY
authors.lastname: Moon
authors.forename: Won Kyong
authors.initials: WK
authors.lastname: Kim
authors.forename: Suk
authors.initials: S
authors.lastname: Kim
authors.forename: Jin
authors.initials: J
authors.lastname: Hur
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides; D002004:Brucella Vaccine / Q000276:immunology* / Q000378:metabolism; D002003:Brucella abortus / Q000276:immunology* / Q000378:metabolism; D002006:Brucellosis / Q000517:prevention & control*; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D013552:Swine
chemical_list: D023181:Antimicrobial Cationic Peptides; D002004:Brucella Vaccine; D016207:Cytokines; C085474:PMAP-36; D010446:Peptide Fragments; D011506:Proteins