title: Effect of purification method of β-chitin from squid pen on the properties of β-chitin nanofibers.
abstract: The relationship between purification methods of β-chitin from squid pen and the physicochemical properties of β-chitin nanofibers (NFs) were investigated. Two types of β-chitin were prepared, with β-chitin (a→b) subjected to acid treatment for decalcification and then base treatment for deproteinization, while β-chitin (b→a) was treated in the opposite order. These β-chitins were disintegrated into NFs using wet pulverization. The β-chitin (b→a) NF dispersion has higher transmittance and viscosity than the β-chitin (a→b) NF dispersion. For the first time, we succeeded in obtaining 3D images of the β-chitin NF dispersion in water by using quick-freeze deep-etch replication with high-angle annular dark field scanning transmission electron microscopy. The β-chitin (b→a) NF dispersion has a denser and more uniform 3D network structure than the β-chitin (a→b) NF dispersion. Widths of the β-chitin (a→b) and (b→a) NFs were approximately 8-25 and 3-10nm, respectively.
authors.affiliation: Department of Chemistry and Materials, Faculty of Textile Science and Technology, Shinshu University, 3-15-1, Tokida, Ueda, Nagano 386-8567, Japan.
authors.forename: Shin
authors.initials: S
authors.lastname: Suenaga
authors.affiliation: Department of Chemical Engineering, National Institute of Technology, Ichinoseki College, Takanashi, Hagisho, Ichinoseki, Iwate 021-8511, Japan.
authors.forename: Nozomi
authors.initials: N
authors.lastname: Nikaido
authors.affiliation: Department of Chemical Engineering, National Institute of Technology, Ichinoseki College, Takanashi, Hagisho, Ichinoseki, Iwate 021-8511, Japan.
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Totani
authors.affiliation: National Institute of Advanced Science and Technology (AIST), 1-8-31, Midorigaoka, Ikeda, Osaka 563-8577, Japan.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Kawasaki
authors.affiliation: Iwate Industrial Research Institute, 2-4-25, Kitaiioka, Morioka, Iwate 020-0857, Japan.
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Ito
authors.affiliation: Yaegaki Bio-Industry, Inc., 681, Mukudani, Hayashida, Himeji, Hyogo 678-4298, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Yamashita
authors.affiliation: Department of Chemistry and Materials, Faculty of Textile Science and Technology, Shinshu University, 3-15-1, Tokida, Ueda, Nagano 386-8567, Japan. Electronic address: osadam@shinshu-u.ac.jp.
authors.forename: Mitsumasa
authors.initials: M
authors.lastname: Osada
mesh_terms: D000818:Animals; D002686:Chitin / Q000737:chemistry / Q000302:isolation & purification* / Q000648:ultrastructure; D049832:Decapodiformes / Q000033:anatomy & histology*; D008970:Molecular Weight; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D011208:Powders; D014783:Viscosity; D014867:Water / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D011208:Powders; D014867:Water; D002686:Chitin
keywords: Chitin; Nanofiber; Star Burst