title: Angiogenesis correlates with macrophage and mast cell infiltration in lung tissue of animals exposed to fluoro-edenite fibers.
abstract: Angiogenesis plays a crucial role in progression of pleural malignant mesothelioma. A significantly increased incidence of pleural mesothelioma has been attributed to exposure to fluoro-edenite, a fibrous amphibole extracted from a local stone quarry. In this study, we have investigated the expression of CD68-positive macrophages, tryptase-positive mast cells and CD31 positive areas, as expression of microvascular density, in lung tissue of sheeps exposed to fluoro-edenite fibers vs controls, by immunohistochemical, morphometric and Western blot analysis. The result have evidenced a significant increase in the expression of CD68-positive macrophages, tryptase-positive mast cells as well as a significant increase in microvascular density evaluated as CD31 positive areas in lung tissue of of sheeps exposed to fluoro-edenite fibers vs controls. These data confirmed the important role played by tumor microenvironment components, including macrophages and mast cells, in favour of angiogenesis in pleural mesothelioma induced by fluoro-edenite exposure.
authors.affiliation: Department of Biomedical Sciences and Biotechnologies, Human Anatomy and Histology Section, School of Medicine, University of Catania, Catania, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Musumeci
authors.affiliation: Department of Biomedical Sciences and Biotechnologies, Human Anatomy and Histology Section, School of Medicine, University of Catania, Catania, Italy.
authors.forename: Carla
authors.initials: C
authors.lastname: Loreto
authors.affiliation: Department of Biomedical Sciences and Biotechnologies, Human Anatomy and Histology Section, School of Medicine, University of Catania, Catania, Italy.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Giunta
authors.affiliation: Occupational Medicine, Department of Clinical and Experimental Medicine, University of Catania, Catania, Italy.
authors.forename: Venerando
authors.initials: V
authors.lastname: Rapisarda
authors.affiliation: Department of Biomedical Sciences and Biotechnologies, Human Anatomy and Histology Section, School of Medicine, University of Catania, Catania, Italy.
authors.forename: Marta Anna
authors.initials: MA
authors.lastname: Szychlinska
authors.affiliation: Department of Biomedical Sciences and Biotechnologies, Human Anatomy and Histology Section, School of Medicine, University of Catania, Catania, Italy.
authors.forename: Rosa
authors.initials: R
authors.lastname: Imbesi
authors.affiliation: Department of Biomedical Sciences and Biotechnologies, Human Anatomy and Histology Section, School of Medicine, University of Catania, Catania, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Castorina
authors.affiliation: Department of Basic Medical Sciences, Neurosciences and Sensory Organs, University of Bari, Medical School, Policlinico - Piazza G. Cesare, 11, 70124 Bari, Italy.
authors.forename: Tiziana
authors.initials: T
authors.lastname: Annese
authors.affiliation: Department of Biomedical Sciences and Biotechnologies, Human Anatomy and Histology Section, School of Medicine, University of Catania, Catania, Italy.
authors.forename: Sergio
authors.initials: S
authors.lastname: Castorina
authors.affiliation: Department of Biomedical Sciences and Biotechnologies, Human Anatomy and Histology Section, School of Medicine, University of Catania, Catania, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Castrogiovanni
authors.affiliation: Department of Basic Medical Sciences, Neurosciences and Sensory Organs, University of Bari, Medical School, Policlinico - Piazza G. Cesare, 11, 70124 Bari, Italy; National Cancer Institute "Giovanni Paolo II", 70124 Bari, Italy. Electronic address: domenico.ribatti@uniba.it.
authors.forename: Domenico
authors.initials: D
authors.lastname: Ribatti
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D017636:Asbestos, Amphibole / Q000633:toxicity*; D015153:Blotting, Western; D003720:Densitometry; D005260:Female; D007150:Immunohistochemistry; D008168:Lung / Q000187:drug effects / Q000473:pathology*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D018919:Neovascularization, Physiologic / Q000187:drug effects; D012756:Sheep; D013194:Staining and Labeling; D053802:Tryptases / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D017636:Asbestos, Amphibole; D014404:Tubulin; C492470:fluor-edenite; D053802:Tryptases
keywords: Angiogenesis; Fluoro-edenite; Macrophages; Mast cells; Mesothelioma