title: Clinic significance of markedly decreased α-klothoin women with preeclampsia.
abstract: Preeclampsia (PE) is a leading cause of maternal and perinatal morbidity and mortality. Klotho is a novel gene and the secret form, α-klotho (α-KL), is related to preeclampsia. We conducted this cross-sectional study in Wuhan, China. We used immunohistochemistry, real-time PCR, western blot, ELISA to measure α-KL expression in placenta and its secretion in maternal and umbilical cord serum, and analyzed correlations between α-KL level and other parameters in normal and preeclampsia pregnancy. We found that both mRNA and protein expression of placental α-KL in women with PE was significantly lower than that in normal pregnancy. Also, expression level of α-KL in both maternal and umbilical cord was markedly decreased in PE patients. Further analyses showed that serum α-KL exhibited positive association with fetal birth weight, and reverse association with oxidative stress and renal function markers. Receiver operating characteristic analysis suggested α-KL might be a potential predictor for preeclampsia.
authors.affiliation: Department of Obstetrics and Gynecology, Renmin Hospital, Wuhan University Hubei, 430060, China.
authors.forename: Cuifang
authors.initials: C
authors.lastname: Fan
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Wuhan University 185, Donghu Rd, Wuhan, Hubei, 430071, China.
authors.forename: Yueqiao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Wuhan University 185, Donghu Rd, Wuhan, Hubei, 430071, China.
authors.forename: Jingyi
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Obstetrics and Gynecology, Renmin Hospital, Wuhan University Hubei, 430060, China.
authors.forename: Di
authors.initials: D
authors.lastname: Lei
authors.affiliation: Department of Obstetrics and Gynecology, Renmin Hospital, Wuhan University Hubei, 430060, China.
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Wuhan University 185, Donghu Rd, Wuhan, Hubei, 430071, China.
authors.forename: Sicong
authors.initials: S
authors.lastname: Lei
authors.affiliation: Department of Obstetrics and Gynecology, Renmin Hospital, Wuhan University Hubei, 430060, China.
authors.forename: Min
authors.initials: M
authors.lastname: Hu
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Wuhan University 185, Donghu Rd, Wuhan, Hubei, 430071, China.
authors.forename: Yatao
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Wuhan University 185, Donghu Rd, Wuhan, Hubei, 430071, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Li
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Wuhan University 185, Donghu Rd, Wuhan, Hubei, 430071, China.
authors.forename: Suqing
authors.initials: S
authors.lastname: Wang
keywords: Preeclampsia; fetal birth weight; oxidative stress; receiver operating characteristic; renal function; α-klotho