title: Anti-biofilm properties of the antimicrobial peptide temporin 1Tb and its ability, in combination with EDTA, to eradicate Staphylococcus epidermidis biofilms on silicone catheters.
abstract: In search of new antimicrobials with anti-biofilm potential, in the present study activity of the frog-skin derived antimicrobial peptide temporin 1Tb (TB) against Staphylococcus epidermidis biofilms was investigated. A striking ability of TB to kill both forming and mature S. epidermidis biofilms was observed, especially when the peptide was combined with cysteine or EDTA, respectively. Kinetics studies demonstrated that the combination TB/EDTA was active against mature biofilms already after 2-4-h exposure. A double 4-h exposure of biofilms to TB/EDTA further increased the therapeutic potential of the same combination. Of note, TB/EDTA was able to eradicate S. epidermidis biofilms formed in vitro on silicone catheters. At eradicating concentrations, TB/EDTA did not cause hemolysis of human erythrocytes. The results shed light on the anti-biofilm properties of TB and suggest a possible application of the peptide in the lock therapy of catheters infected with S. epidermidis.
authors.affiliation: a Department of Translational Research and new Technologies in Medicine and Surgery , University of Pisa , Pisa , Italy.
authors.forename: Giuseppantonio
authors.initials: G
authors.lastname: Maisetta
authors.affiliation: a Department of Translational Research and new Technologies in Medicine and Surgery , University of Pisa , Pisa , Italy.
authors.forename: Lucia
authors.initials: L
authors.lastname: Grassi
authors.affiliation: b NEST, Istituto Nanoscienze-CNR and Scuola Normale Superiore , Pisa , Italy.|c Center for Musculoskeletal Surgery, Septic Surgical Unit , Charité University Medicine , Berlin , Germany.
authors.forename: Mariagrazia
authors.initials: M
authors.lastname: Di Luca
authors.affiliation: a Department of Translational Research and new Technologies in Medicine and Surgery , University of Pisa , Pisa , Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Bombardelli
authors.affiliation: a Department of Translational Research and new Technologies in Medicine and Surgery , University of Pisa , Pisa , Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Medici
authors.affiliation: a Department of Translational Research and new Technologies in Medicine and Surgery , University of Pisa , Pisa , Italy.
authors.forename: Franca Lisa
authors.initials: FL
authors.lastname: Brancatisano
authors.affiliation: a Department of Translational Research and new Technologies in Medicine and Surgery , University of Pisa , Pisa , Italy.
authors.forename: Semih
authors.initials: S
authors.lastname: Esin
authors.affiliation: a Department of Translational Research and new Technologies in Medicine and Surgery , University of Pisa , Pisa , Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Batoni
mesh_terms: D000890:Anti-Infective Agents / Q000008:administration & dosage / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides; D018441:Biofilms / Q000187:drug effects*; D057785:Catheters / Q000382:microbiology*; D004492:Edetic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D011506:Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D012828:Silicones / Q000737:chemistry*; D013212:Staphylococcus epidermidis / Q000187:drug effects* / Q000502:physiology
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D011506:Proteins; D012828:Silicones; C104501:temporin; D004492:Edetic Acid
keywords: EDTA; Staphylococcus epidermidis; Temporin; antimicrobial peptides; biofilm; lock therapy