title: Lactobacillus curvatus WiKim38 isolated from kimchi induces IL-10 production in dendritic cells and alleviates DSS-induced colitis in mice.
abstract: Probiotics such as lactobacilli and bifidobacteria have healthpromoting effects by immune modulation. In the present study, we examined the immunomodulatory properties of Lactobacillus curvatus WiKim38, which was newly isolated from baechu (Chinese cabbage) kimchi. The ability of L. curvatus WiKim38 to induce cytokine production in bone marrow-derived dendritic cells (BMDCs) was determined by enzyme-linked immunosorbent assay. To evaluate the molecular mechanisms underlying L. curvatus Wikim38-mediated IL-10 production, Western blot analyses and inhibitor assays were performed. Moreover, the in vivo anti-inflammatory effects of L. curvatus WiKim38 were examined in a dextran sodium sulfate (DSS)-induced colitis mouse model. L. curvatus WiKim38 induced significantly higher levels of IL-10 in BMDCs compared with that induced by LPS. NF-κB and ERK were activated by L. curvatus WiKim38, and an inhibitor assay revealed that these pathways were required for L. curvatus WiKim38-induced production of IL-10 in BMDCs. An in vivo experiment showed that oral administration of L. curvatus WiKim38 increased the survival rate of mice with DSS-induced colitis and improved clinical signs and histopathological severity in colon tissues. Taken together, these results indicate that L. curvatus Wikim38 may have health-promoting effects via immune modulation, and may thus be applicable for therapy of various inflammatory diseases.
authors.affiliation: Laboratory Animal Medicine and BK21 PLUS Project Team, College of Veterinary Medicine, Chonnam National University, Gwangju, 61186, Republic of Korea.
authors.forename: Sung-Gang
authors.initials: SG
authors.lastname: Jo
authors.affiliation: Department of Biochemistry, College of Medicine, Konyang University, Daejeon, 35365, Republic of Korea.
authors.forename: Eui-Jeong
authors.initials: EJ
authors.lastname: Noh
authors.affiliation: Laboratory Animal Medicine and BK21 PLUS Project Team, College of Veterinary Medicine, Chonnam National University, Gwangju, 61186, Republic of Korea.
authors.forename: Jun-Young
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Laboratory Animal Medicine and BK21 PLUS Project Team, College of Veterinary Medicine, Chonnam National University, Gwangju, 61186, Republic of Korea.|National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungbuk, 28116, Republic of Korea.
authors.forename: Green
authors.initials: G
authors.lastname: Kim
authors.affiliation: Laboratory Animal Medicine and BK21 PLUS Project Team, College of Veterinary Medicine, Chonnam National University, Gwangju, 61186, Republic of Korea.
authors.forename: Joo-Hee
authors.initials: JH
authors.lastname: Choi
authors.affiliation: Microbiology and Functionality Research Group, World Institute of Kimchi, Gwangju, 61755, Republic of Korea.
authors.forename: Mo-Eun
authors.initials: ME
authors.lastname: Lee
authors.affiliation: Microbiology and Functionality Research Group, World Institute of Kimchi, Gwangju, 61755, Republic of Korea.
authors.forename: Jung-Hee
authors.initials: JH
authors.lastname: Song
authors.affiliation: Microbiology and Functionality Research Group, World Institute of Kimchi, Gwangju, 61755, Republic of Korea. jychang@wikim.re.kr.
authors.forename: Ji-Yoon
authors.initials: JY
authors.lastname: Chang
authors.affiliation: Laboratory Animal Medicine and BK21 PLUS Project Team, College of Veterinary Medicine, Chonnam National University, Gwangju, 61186, Republic of Korea. jonpark@jnu.ac.kr.
authors.forename: Jong-Hwan
authors.initials: JH
authors.lastname: Park
mesh_terms: D000818:Animals; D001937:Brassica / Q000382:microbiology*; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000276:immunology* / Q000382:microbiology; D003713:Dendritic Cells / Q000276:immunology* / Q000382:microbiology; D016264:Dextran Sulfate / Q000009:adverse effects; D004195:Disease Models, Animal; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics / Q000276:immunology*; D007778:Lactobacillus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D008810:Mice, Inbred C57BL; D019936:Probiotics / Q000008:administration & dosage*; D014675:Vegetables / Q000382:microbiology
chemical_list: D016753:Interleukin-10; D016264:Dextran Sulfate
keywords: Lactobacillus curvatus; colitis; dendritic cells; interleukin-10; kimchi