title: Thermo-Controlled in Situ Phase Transition of Polymer-Peptides on Cell Surfaces for High-Performance Proliferative Inhibition.
abstract: We herein report a thermocontrolled strategy for realizing in situ conformational transition of polymer-peptide conjugates at cell surfaces to manipulate and monitor HER2 receptor clustering, which finally result in effective breast cancer cell proliferation inhibition. Functional paring motifs (HBP) are covalently linked to a synthetic thermoresponsive polymer PNIPAAm to incorporate temperature control properties to HER2 targeting peptide. At 40 °C, the PNIPAAm polymers collapse and act as a "shield" to block the aggregation of HBP. Upon cooling to 35 °C, polymers are in their extended state and HBP are expose in aqueous and aggregate subsequently with enhanced fluorescence, allowing for promoting and in situ monitoring of receptor clustering. Ultimately, HER2 receptor clustering leads to cytoplasmic domain phosphorylation, which further results in effective cancer cell proliferation inhibition. We envision that this useful approach has the potential to be applied for molecule-targeted tumor therapy.
authors.affiliation: CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST) , Beijing, 100190, P. R. China.|University of Chinese Academy of Science (UCAS) , No. 19A Yuquan Road, Beijing, 100049, P. R. China.
authors.forename: Sheng-Lin
authors.initials: SL
authors.lastname: Qiao
authors.affiliation: CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST) , Beijing, 100190, P. R. China.|University of Chinese Academy of Science (UCAS) , No. 19A Yuquan Road, Beijing, 100049, P. R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST) , Beijing, 100190, P. R. China.|University of Chinese Academy of Science (UCAS) , No. 19A Yuquan Road, Beijing, 100049, P. R. China.
authors.forename: Yao-Xin
authors.initials: YX
authors.lastname: Lin
authors.affiliation: CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST) , Beijing, 100190, P. R. China.|University of Chinese Academy of Science (UCAS) , No. 19A Yuquan Road, Beijing, 100049, P. R. China.
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: An
authors.affiliation: CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST) , Beijing, 100190, P. R. China.|University of Chinese Academy of Science (UCAS) , No. 19A Yuquan Road, Beijing, 100049, P. R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Ma
authors.affiliation: CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST) , Beijing, 100190, P. R. China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Li
authors.affiliation: CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST) , Beijing, 100190, P. R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST) , Beijing, 100190, P. R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
mesh_terms: D000180:Acrylic Resins; D008968:Molecular Conformation; D010455:Peptides; D044367:Phase Transition; D011108:Polymers; D013696:Temperature
chemical_list: D000180:Acrylic Resins; D010455:Peptides; D011108:Polymers
keywords: cancer; peptide; polymer; self-assembly; temperature-responsive