title: [EFFECT OF SHORT-TERM DRY IMMERSION ON PROTEOLYTIC SIGNALING IN HUMAN SOLEUS MUSCLE].
abstract: The signaling processes initiating proteolytic events in m. soleus of humans during short-term exposure in the non-weight bearing conditions were analyzed. Dry immersion (DI) was used to induce weight deprivation over 3 days. Western blotting was used to define the IRS-1 content, total and phosphorylated neuronal NO-synthase (nNOS), AMP-activated protein kinase (AMPK) that control the anabolic and catabolic pathways, and concentrations of cytoskeletal protein desmin and Ca²⁺-activated protease calpin. Already on day-3 of DI calpain-dependent proteolysis manifests itself by reductions in both the total content and level of nNOS phosphorilation. Moreover, AMPK phosphorilation was decreased drastically.
authors.lastname: Vil'chinskaya N A
authors.forename: T M
authors.initials: TM
authors.lastname: Mirzoev
authors.forename: Yu N
authors.initials: YN
authors.lastname: Lomonosova
authors.forename: I B
authors.initials: IB
authors.lastname: Kozlovskaya
authors.forename: B S
authors.initials: BS
authors.lastname: Shenkman
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000096:biosynthesis*; D002154:Calpain / Q000096:biosynthesis; D003893:Desmin / Q000096:biosynthesis; D006801:Humans; D007101:Immersion; D055504:Insulin Receptor Substrate Proteins / Q000096:biosynthesis; D008660:Metabolism / Q000235:genetics; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000502:physiology; D052248:Nitric Oxide Synthase Type I / Q000096:biosynthesis*; D059748:Proteolysis
chemical_list: D003893:Desmin; C526292:IRS1 protein, human; D055504:Insulin Receptor Substrate Proteins; D052248:Nitric Oxide Synthase Type I; D055372:AMP-Activated Protein Kinases; D002154:Calpain