title: Prussian blue/serum albumin/indocyanine green as a multifunctional nanotheranostic agent for bimodal imaging guided laser mediated combinatorial phototherapy.
abstract: Developing novel nanotheranostic agent using only clinically approved materials is highly desirable and challenging. In this study, we combined three clinically approved materials, Prussian blue (PB), serum albumin (BSA), and indocyanine green (ICG), by a simple and biocompatible method to prepare a multifunctional theranostic PB-BSA-ICG nanoparticle. The multifunctional nanoparticle system could provide dual mode magnetic resonance (MR) and near infrared (NIR) fluorescence imaging as well as combined photothermal and photodynamic (PTT-PDT) therapy in response to a single NIR laser. This nanoparticle showed an excellent stability in physiological solutions and could suppress the photo-instability of ICG. In the absence of light, the nanoparticles showed no cytotoxicity, but significant cell death was induced through combined PTT-PDT effect after irradiation with NIR laser light. A high tumor accumulation and minimal nonspecific uptake by other major organs of PB-BSA-ICG nanoparticle were observed in vivo, analyzed by T1-weighted MR and NIR fluorescence bimodal imaging in tumor xenograft mice after intravenous injection. The nanoparticles efficiently suppressed the tumor growth through combinatorial phototherapy with no tumor recurrence upon a single NIR laser irradiation. These results demonstrated that PB-BSA-ICG is potentially an interesting nanotheranostic agent for imaging guided cancer therapy by overcoming the limitations of each technology and enhancing the therapeutic efficiency as well as reducing side effects.
authors.affiliation: School of Materials Science and Engineering, Gwangju Institute of Science and Technology, 261 Cheomdan-gwagiro, Buk-gu, Gwangju 61005, Republic of Korea.
authors.forename: Abhishek
authors.initials: A
authors.lastname: Sahu
authors.affiliation: School of Materials Science and Engineering, Gwangju Institute of Science and Technology, 261 Cheomdan-gwagiro, Buk-gu, Gwangju 61005, Republic of Korea.
authors.forename: Jong Hyun
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Radiology, Chonnam National University Medical School and Chonnam National University Hwasun Hospital, Gwangju 61469, Republic of Korea.
authors.forename: Hye Gyeong
authors.initials: HG
authors.lastname: Lee
authors.affiliation: Department of Radiology, Chonnam National University Medical School and Chonnam National University Hwasun Hospital, Gwangju 61469, Republic of Korea.
authors.forename: Yong Yeon
authors.initials: YY
authors.lastname: Jeong
authors.affiliation: School of Materials Science and Engineering, Gwangju Institute of Science and Technology, 261 Cheomdan-gwagiro, Buk-gu, Gwangju 61005, Republic of Korea; Center for Theragnosis, Biomedical Research Institute, KIST, Seoul 02792, Republic of Korea. Electronic address: gytae@gist.ac.kr.
authors.forename: Giyoong
authors.initials: G
authors.lastname: Tae
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D005295:Ferrocyanides / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007208:Indocyanine Green / Q000737:chemistry*; D007834:Lasers; D008027:Light; D008297:Male; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000737:chemistry*; D009369:Neoplasms / Q000000981:diagnostic imaging / Q000628:therapy; D061848:Optical Imaging; D010316:Particle Size; D010789:Phototherapy / Q000379:methods*; D012709:Serum Albumin / Q000737:chemistry*; D000068936:Theranostic Nanomedicine
chemical_list: D005295:Ferrocyanides; D005456:Fluorescent Dyes; D012709:Serum Albumin; D007208:Indocyanine Green; C000170:ferric ferrocyanide
keywords: Indocyanine green; Multifunctional nanoparticle; Photodynamic therapy; Photothermal therapy; Prussian blue; Theranostic