title: Bioorthogonal Chemical Reporters for Monitoring Unsaturated Fatty-Acylated Proteins.
abstract: Dietary unsaturated fatty acids, such as oleic acid, have been shown to be covalently incorporated into a small subset of proteins, but the generality and diversity of this protein modification has not been studied. We synthesized unsaturated fatty-acid chemical reporters and determined their protein targets in mammalian cells. The reporters can induce the formation of lipid droplets and be incorporated site-specifically onto known fatty-acylated proteins and label many proteins in mammalian cells. Quantitative proteomics analysis revealed that unsaturated fatty acids modify similar protein targets to saturated fatty acids, including several immunity-associated proteins. This demonstrates that unsaturated fatty acids can directly modify many proteins to exert their unique and often beneficial physiological effects in vivo.
authors.affiliation: Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, 1230 York Avenue, New York, NY, 10065, USA.|The Crick Institute, 215 Euston Road, London, NW1 2BE, UK.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Thinon
authors.affiliation: Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, 1230 York Avenue, New York, NY, 10065, USA.
authors.forename: Avital
authors.initials: A
authors.lastname: Percher
authors.affiliation: Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, 1230 York Avenue, New York, NY, 10065, USA. hhang@rockefeller.edu.
authors.forename: Howard C
authors.initials: HC
authors.lastname: Hang
mesh_terms: D000215:Acylation; D000818:Animals; D002460:Cell Line; D005231:Fatty Acids, Unsaturated / Q000737:chemistry / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D011506:Proteins / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism; D040901:Proteomics
chemical_list: D005231:Fatty Acids, Unsaturated; D011506:Proteins
keywords: chemical reporter; fatty acids; fatty-acylation; fluorescent probes; quantitative proteomics; unsaturated fatty acids