title: Efficiency and Impact of Positive and Negative Magnetic Separation on Monocyte Derived Dendritic Cell Generation.
abstract: BACKGROUND
  The immunomagnetic separation technique is the basis of monocyte isolation and further generation of monocyte-derived dendritic cells.
  
  
  OBJECTIVE
  To compare the efficiency of monocyte positive and negative separation, concentration of beads, and their impact on generated dendritic cells.
  
  
  METHODS
  Monocytes were obtained using monoclonal antibody-coated magnetic beads followed the Ficoll-Paque gradient separation of mononuclear cell fraction from the peripheral blood of 6 healthy volunteers. CD14 expression was analyzed by flow cytometry.
  
  
  CONCLUSIONS
  Both types of magnetic separation including recommended and reduced concentrations of beads did not affect the yield and the purity of monocytes and their surface CD14 expression. However, DCs originated from the "positively" separated monocytes had noticeable higher expression of CD80.
authors.affiliation: Department of Immunology and Infectious Biology, University of Lodz, Lodz, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Kowalewicz-Kulbat
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Ograczyk
authors.forename: Marcin
authors.initials: M
authors.lastname: Włodarczyk
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Krawczyk
authors.forename: Marek
authors.initials: M
authors.lastname: Fol
mesh_terms: D000328:Adult; D002452:Cell Count; D002454:Cell Differentiation; D003362:Cost-Benefit Analysis; D003713:Dendritic Cells / Q000166:cytology*; D005434:Flow Cytometry; D006801:Humans; D018189:Immunomagnetic Separation / Q000191:economics / Q000379:methods*; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D009000:Monocytes / Q000166:cytology*
chemical_list: D018950:Lipopolysaccharide Receptors