title: Mutations in the drug resistance-determining region of Mycobacterium lepromatosis isolated from leprosy patients in Mexico.
abstract: Mycobacterium lepromatosis, an independent species from Mycobacterium leprae, has been found to be a causative agent for diffuse lepromatous leprosy (DLL) in Mexico, but remains poorly studied. Here, the drug resistance-determining regions (DRDR) of folP1, rpoB and gyrA (conferring resistance to dapsone, rifampicin and quinolone, respectively) in M. lepromatosis from leprosy patients in Mexico were characterized. No mutations or silent mutations were found at previously characterized major sites in DRDR of M. lepromatosis. However, a non-synonymous mutation was found in codon 54 between two major sites of the folP1 DRDR in M. lepromatosis sequences. All M. lepromatosis isolates showed CAG sequence in codon 54 of folP1. Because the next codons 53 and 55 are known as major mutation sites for drug resistance, more detailed analysis using more samples is needed to determine whether it influences susceptibility to dapsone and/or efficiency of folate biosynthesis in M. lepromatosis or not.
authors.affiliation: Department of Mycobacteriology, Leprosy Research Center, National Institute of Infectious Diseases, Tokyo, Japan. mkai@nih.go.jp.
authors.forename: Masanori
authors.initials: M
authors.lastname: Kai
authors.affiliation: University of Guadalajara/Instituto Dermatologico de Jalisco, Guadalajara, Jalisco, Mexico.
authors.forename: Mary
authors.initials: M
authors.lastname: Fafutis-Morris
authors.affiliation: Department of Mycobacteriology, Leprosy Research Center, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Miyamoto
authors.affiliation: Department of Mycobacteriology, Leprosy Research Center, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Tetsu
authors.initials: T
authors.lastname: Mukai
authors.affiliation: University of Guadalajara/Instituto Dermatologico de Jalisco, Guadalajara, Jalisco, Mexico.
authors.forename: Jorge
authors.initials: J
authors.lastname: Mayorga-Rodriguez
authors.affiliation: University of Guadalajara/Instituto Dermatologico de Jalisco, Guadalajara, Jalisco, Mexico.
authors.forename: Marco Antonio
authors.initials: MA
authors.lastname: Rodriguez-Castellanos
authors.affiliation: University of Guadalajara/Instituto Dermatologico de Jalisco, Guadalajara, Jalisco, Mexico.
authors.forename: Marco Alonso
authors.initials: MA
authors.lastname: Martínez-Guzman
authors.affiliation: Department of Mycobacteriology, Leprosy Research Center, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Matsuoka
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001483:Base Sequence; D002648:Child; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D005260:Female; D006801:Humans; D015440:Leprosy, Lepromatous / Q000382:microbiology*; D008297:Male; D008800:Mexico; D008875:Middle Aged; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000235:genetics*; D017422:Sequence Analysis, DNA; D055815:Young Adult
keywords: 
  Mycobacterium leprae
  ; 
  Mycobacterium lepromatosis
  ; 
  folP1
  ; diffuse lepromatous leprosy; drug resistance