title: Episomal plasmid-based generation of induced pluripotent stem cells from fetal femur-derived human mesenchymal stromal cells.
abstract: Human bone mesenchymal stromal cells derived from fetal femur 55 days post-conception were reprogrammed to induced pluripotent stem cells using episomal plasmid-based expression of OCT4, SOX2, NANOG, LIN28, SV40LT, KLF4 and c-MYC and supplemented with the following pathway inhibitors - TGFβ receptor inhibitor (A-83-01), MEK inhibitor (PD325901), GSK3β inhibitor (CHIR99021) and ROCK inhibitor (HA-100). Successful induction of pluripotency in two iPS-cell lines was demonstrated in vitro and by the Pluritest.
authors.affiliation: Institute for Stem Cell Research and Regenerative Medicine, Heinrich Heine University Duesseldorf, Moorenstr. 5, 40225 Duesseldorf, Germany; Max Planck Institute for Molecular Genetics, Ihnestraße 63-73, 14195 Berlin, Germany; Department of Biology, Chemistry and Pharmacy, Institute of Chemistry and Biochemistry, Freie Universität Berlin, Thielallee 63, 14195, Berlin, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Megges
authors.affiliation: Centre for Human Development, Stem Cells and Regeneration, Institute of Developmental Sciences, University of Southampton, Southampton SO16 6YD, UK.
authors.forename: Richard O C
authors.initials: RO
authors.lastname: Oreffo
authors.affiliation: Institute for Stem Cell Research and Regenerative Medicine, Heinrich Heine University Duesseldorf, Moorenstr. 5, 40225 Duesseldorf, Germany; Max Planck Institute for Molecular Genetics, Ihnestraße 63-73, 14195 Berlin, Germany.
authors.forename: James
authors.initials: J
authors.lastname: Adjaye
mesh_terms: D001549:Benzamides / Q000494:pharmacology; D002460:Cell Line; D065150:Cellular Reprogramming; D004159:Diphenylamine / Q000031:analogs & derivatives / Q000494:pharmacology; D005269:Femur / Q000166:cytology*; D005333:Fetus / Q000166:cytology; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D059785:Karyotype; D000090062:Kruppel-Like Factor 4; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D010957:Plasmids / Q000378:metabolism; D011720:Pyrazoles / Q000494:pharmacology; D011725:Pyridines / Q000494:pharmacology; D011743:Pyrimidines / Q000494:pharmacology; D013882:Thiosemicarbazones / Q000494:pharmacology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014162:Transfection
chemical_list: C507011:A-83-01; D001549:Benzamides; C473711:Chir 99021; C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4; D011720:Pyrazoles; D011725:Pyridines; D011743:Pyrimidines; D013882:Thiosemicarbazones; D014157:Transcription Factors; C506614:mirdametinib; D004159:Diphenylamine