title: Down-expression of miR-154 suppresses tumourigenesis in CD133(+) glioblastoma stem cells.
abstract: Glioblastoma multiforme (GBM) is the most common and aggressive form of brain cancer. Evidences have suggested that CD133 is a marker for a subset of glioblastoma cancer stem cells. However, whether miRNA plays a critical role in CD133(+) GBM is poorly understood. Here, we identified that miR-154 was upregulated in CD133(+) GBM cell lines. Knockdown of miR-154 remarkably suppressed proliferation and migration of CD133(+) GBM cells. Further study found that PRPS1 was a direct target of miR-154 in CD133(+) GBM cells. Overexpression of PRPS1 exhibited similar effects as miR-154 knockdown in CD133(+) GBMs. Our study identified miR-154 as a previously unrecognized positive regulator of proliferation and migration in CD133(+) GBM cells and a potentially therapeutic target of GBMs. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang, China.
authors.forename: Zhongjie
authors.initials: Z
authors.lastname: Yan
authors.affiliation: Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang, China.
authors.forename: Yuanyu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang, China.
authors.forename: Wandong
authors.initials: W
authors.lastname: Ma
authors.affiliation: Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Li
mesh_terms: D000071916:AC133 Antigen / Q000378:metabolism*; D000818:Animals; D001932:Brain Neoplasms / Q000235:genetics / Q000473:pathology; D063646:Carcinogenesis / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D015536:Down-Regulation / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D005909:Glioblastoma / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D014411:Neoplastic Stem Cells / Q000378:metabolism* / Q000473:pathology; D012268:Ribose-Phosphate Pyrophosphokinase / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation / Q000235:genetics; D023041:Xenograft Model Antitumor Assays
chemical_list: D000071916:AC133 Antigen; C561122:MIRN154 microRNA, human; D035683:MicroRNAs; C510474:PRPS1 protein, human; D012268:Ribose-Phosphate Pyrophosphokinase
keywords: CD133; PRPS1; cell proliferation; glioblastoma stem cells; miR-154