title: Target genes regulated by transcription factor E2F1 in small cell lung cancer.
abstract: Previously, we have reported that transcription factor E2F1 expression is up-regulated in approximately 95% of small cell lung cancer tissue samples and closely associated with invasion and metastasis, but few studies have investigated specific target genes regulated by E2F1 in this disease. The aim of this study was to clarify the target genes controlled by E2F1 in the small cell lung cancer cell line H1688. The results of chromatin immunoprecipitation sequencing (ChIP-seq) showed that total 5 326 potential target genes were identified, in which 4 700 were structural genes and 626 long non-coding RNAs (lncRNAs). Gene Ontology (GO) and enrichment map analysis results indicated that these target genes were associated with three main functions: (1) cell cycle regulation, (2) chromatin and histone modification, and (3) protein transport. MEME4.7.0 software was used to identify the E2F1 binding DNA motif, and six motifs were discovered for coding genes and lncRNAs. These results clarify the target genes of E2F1, and provide the experimental basis for further exploring the roles of E2F1 in tumorigenesis, development, invasion and metastasis, recurrence, and drug resistance in small cell lung cancer.
authors.affiliation: Department of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai 264003, China.
authors.forename: Zun-Ling
authors.initials: ZL
authors.lastname: Li
authors.affiliation: Department of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai 264003, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Jiao
authors.affiliation: Department of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai 264003, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai 264003, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Yue
authors.affiliation: Department of Biochemistry and Molecular Biology, Binzhou Medical University, Yantai 264003, China. yt_klijun@163.com.
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Kong
mesh_terms: D002843:Chromatin; D050687:E2F1 Transcription Factor; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms; D055752:Small Cell Lung Carcinoma; D015854:Up-Regulation
chemical_list: D002843:Chromatin; D050687:E2F1 Transcription Factor; C493753:E2F1 protein, human