title: Improvement of immune responses to influenza vaccine (H5N1) by sulfated yeast beta-glucan.
abstract: In this study, the adjuvant activity of sulfated glucan from saccharomyces cerevisiae (SGSC) was investigated. sGSC had molecular weight of 12.9kDa and degrees of sulfation of 0.16. The effects ofSGSC on the splenic lymphocyte cells of mice were measured in vitro. The results showed thatSGSC could significantly promote lymphocyte proliferation singly or synergistically with Con A and LPS, and stimulate the cells to secrete IL-2 and INF-γ. The adjuvant activity ofSGSC was researched in BALB/c mice inoculated with inactivated H5N1vaccine in vivo. The results showed thatSGSC could significantly enhance lymphocyte proliferation, effectively increase the percentage of CD4+T cells, decrease the percentage of CD8+T cells, and elevate the CD4+/CD8+ratio; enhance the HI antibody titre, and promote the production of IL-2, INF-γ, IL-4 and IL-6 at medium level. These results indicated that sulfated glucan showed an excellent adjuvant effect on H5N1vaccine in a mouse model. Therefore,SGSC could be used as an effective immune adjuvant for an inactivated H5N1vaccine.
authors.affiliation: Department of Pharmacy, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China. Electronic address: wangmi@shvri.ac.cn.
authors.forename: Mi
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
authors.forename: Lifang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Pharmacy, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
authors.forename: Ruile
authors.initials: R
authors.lastname: Yang
authors.affiliation: Department of Pharmacy, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
authors.forename: Chenzhong
authors.initials: C
authors.lastname: Fei
authors.affiliation: Department of Pharmacy, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
authors.forename: Keyu
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Department of Pharmacy, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China.
authors.forename: Wenli
authors.initials: W
authors.lastname: Zheng
authors.affiliation: Department of Pharmacy, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China. Electronic address: feiqun@gmail.com.
authors.forename: Feiqun
authors.initials: F
authors.lastname: Xue
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D016207:Cytokines / Q000097:blood / Q000378:metabolism; D005936:Glucans / Q000494:pharmacology*; D056747:Immunomodulation / Q000276:immunology; D053124:Influenza A Virus, H5N1 Subtype / Q000276:immunology*; D007252:Influenza Vaccines / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012441:Saccharomyces cerevisiae / Q000737:chemistry*; D013154:Spleen / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D016207:Cytokines; D005936:Glucans; D007252:Influenza Vaccines; C078962:glucan sulfate
keywords: Adjuvant activity; H5N1vaccine; Sulfated glucan from saccharomyces cerevisiae