title: CHARACTERIZATION OF NICOTINE ACETYLCHOLINE RECEPTOR SUBUNITS IN THE COCKROACH Periplaneta americana MUSHROOM BODIES REVEALS A STRONG EXPRESSION OF β1 SUBUNIT: INVOLVEMENT IN NICOTINE-INDUCED CURRENTS.
abstract: Nicotinic acetylcholine receptors are ligand-gated ion channels expressed in many insect structures, such as mushroom bodies, in which they play a central role. We have recently demonstrated using electrophysiological recordings that different native nicotinic receptors are expressed in cockroach mushroom bodies Kenyon cells. In the present study, we demonstrated that eight genes coding for cockroach nicotinic acetylcholine receptor subunits are expressed in the mushroom bodies. Quantitative real-time polymerase chain reaction (PCR) experiments demonstrated that β1 subunit was the most expressed in the mushroom bodies. Moreover, antisense oligonucleotides performed against β1 subunit revealed that inhibition of β1 expression strongly decreases nicotine-induced currents amplitudes. Moreover, co-application with 0.5 μM α-bungarotoxin completely inhibited nicotine currents whereas 10 μM d-tubocurarine had a partial effect demonstrating that β1-containing neuronal nicotinic acetylcholine receptor subtypes could be sensitive to the nicotinic acetylcholine receptor antagonist α-bungarotoxin.
authors.affiliation: Laboratoire Récepteurs et Canaux Ioniques Membranaires (RCIM), Université d'Angers, Angers, France.
authors.forename: Emiliane
authors.initials: E
authors.lastname: Taillebois
authors.affiliation: Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), Université d'Orléans, Orléans, France.
authors.forename: Steeve H
authors.initials: SH
authors.lastname: Thany
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002038:Bungarotoxins / Q000494:pharmacology; D003001:Cloning, Molecular; D015870:Gene Expression; D005786:Gene Expression Regulation / Q000187:drug effects; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008297:Male; D024521:Mushroom Bodies / Q000378:metabolism; D010526:Periplaneta / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D011978:Receptors, Nicotinic / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D002038:Bungarotoxins; D019476:Insect Proteins; D012333:RNA, Messenger; D011978:Receptors, Nicotinic
keywords: Kenyon cells; cockroach; nicotinic receptors; β1 subunit