title: Platelet-Rich Plasma Obtained with Different Anticoagulants and Their Effect on Platelet Numbers and Mesenchymal Stromal Cells Behavior In Vitro.
abstract: There are promising results in the use of platelet-rich plasma (PRP) for musculoskeletal tissue repair. However, the variability in the methodology for its obtaining may cause different and opposing findings in the literature. Particularly, the choice of the anticoagulant is the first definition to be made. In this work, blood was collected with sodium citrate (SC), ethylenediaminetetraacetic acid (EDTA), or anticoagulant citrate dextrose (ACD) solution A, as anticoagulants, prior to PRP obtaining. Hematological analysis and growth factors release quantification were performed, and the effects on mesenchymal stromal cell (MSC) culture, such as cytotoxicity and cell proliferation (evaluated by MTT method) and gene expression, were evaluated. The use of EDTA resulted in higher platelet yield in whole blood; however, it induced an increase in the mean platelet volume (MPV) following the blood centrifugation steps for PRP obtaining. The use of SC and ACD resulted in higher induction of MSC proliferation. On the other hand, PRP obtained in SC presented the higher platelet recovery after the blood first centrifugation step and a minimal change in MSC gene expression. Therefore, we suggest the use of SC as the anticoagulant for PRP obtaining.
authors.affiliation: Excellion Serviços Biomédicos, Amil/UnitedHealth Group, 25651-000 Petrópolis, RJ, Brazil; Laboratório de Biologia e Tecnologia Celular, Universidade Veiga de Almeida, 20270-150 Rio de Janeiro, RJ, Brazil.
authors.forename: Ronaldo José Farias Corrêa
authors.initials: RJ
authors.lastname: do Amaral
authors.affiliation: Excellion Serviços Biomédicos, Amil/UnitedHealth Group, 25651-000 Petrópolis, RJ, Brazil.
authors.forename: Nemias Pereira
authors.initials: NP
authors.lastname: da Silva
authors.affiliation: Excellion Serviços Biomédicos, Amil/UnitedHealth Group, 25651-000 Petrópolis, RJ, Brazil.
authors.forename: Natália Ferreira
authors.initials: NF
authors.lastname: Haddad
authors.affiliation: Excellion Serviços Biomédicos, Amil/UnitedHealth Group, 25651-000 Petrópolis, RJ, Brazil.
authors.forename: Luana Siqueira
authors.initials: LS
authors.lastname: Lopes
authors.affiliation: Excellion Serviços Biomédicos, Amil/UnitedHealth Group, 25651-000 Petrópolis, RJ, Brazil.
authors.forename: Fábio Dias
authors.identifier: 0000-0002-6127-0720
authors.initials: FD
authors.lastname: Ferreira
authors.affiliation: Universidade Federal Fluminense, 24033-900 Niterói, RJ, Brazil.
authors.forename: Ricardo Bastos
authors.identifier: 0000-0002-8207-6708
authors.initials: RB
authors.lastname: Filho
authors.affiliation: Excellion Serviços Biomédicos, Amil/UnitedHealth Group, 25651-000 Petrópolis, RJ, Brazil.
authors.forename: Paola Alejandra
authors.initials: PA
authors.lastname: Cappelletti
authors.affiliation: Excellion Serviços Biomédicos, Amil/UnitedHealth Group, 25651-000 Petrópolis, RJ, Brazil; Centro Universitário Celso Lisboa, 20950-091 Rio de Janeiro, RJ, Brazil.
authors.forename: Wallace
authors.initials: W
authors.lastname: de Mello
authors.affiliation: Laboratório de Biologia e Tecnologia Celular, Universidade Veiga de Almeida, 20270-150 Rio de Janeiro, RJ, Brazil.
authors.forename: Eric
authors.identifier: 0000-0002-6840-2183
authors.initials: E
authors.lastname: Cordeiro-Spinetti
authors.affiliation: Excellion Serviços Biomédicos, Amil/UnitedHealth Group, 25651-000 Petrópolis, RJ, Brazil; Laboratório de Biologia e Tecnologia Celular, Universidade Veiga de Almeida, 20270-150 Rio de Janeiro, RJ, Brazil.
authors.forename: Alex
authors.initials: A
authors.lastname: Balduino