title: [Insulin combined with selenium inhibit p38MAPK/CBP pathway and suppresses cardiomyocyte apoptosis in rats with diabetic cardiomyopathy].
abstract: Objective To investigate the effect of insulin in combination with selenium on p38-mitogen-activated protein kinase/CREB-binding protein (p38MAPK/CBP) pathway in rats with diabetic cardiomyopathy. Methods Fifty SD rats were randomly grouped into control group, diabetic cardiomyopathy (DCM) group, diabetic cardiomyopathy with insulin treatment (DCM-In) group, diabetic cardiomyopathy with selenium treatment (DCM-Se) group, and diabetic cardiomyopathy with insulin and selenium combination treatment (DCM-In-Se) group. Flow cytometry was used to analyze cell cycle. TUNEL staining was used to detect cardiomyocyte apoptosis. Western blotting was used to examine the levels of cyclin D1, cyclin E, Bax, Bcl-2, p38MAPK, p-p38MAPK, CBP and Ku70. Co-immunoprecipitation was used to examine the acetylation status of Ku70. Results Insulin in combination with selenium significantly inhibited cardiomyocyte apoptosis, increased Bcl-2 levels and decreased Bax, cyclin D1, cyclin E, p38MAPK, p-p38MAPK, CBP, Ku70 and acetylated Ku70 levels. Conclusion The combined treatment of insulin and selenium suppresses cardiomyocyte apoptosis by inhibiting p38MAPK/CBP pathway.
authors.affiliation: Department of Pharmacology and Toxicology, Xi'an Medical University, Xi'an 710021, China. *Corresponding author, E-mail: xtj1118@163.com.
authors.forename: Tianjiao
authors.initials: T
authors.lastname: Xu
authors.affiliation: Department of Geriatrics, Xianyang Hospital, Yan'an University, Xianyang 712000, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Pharmacology and Toxicology, Xi'an Medical University, Xi'an 710021, China.
authors.forename: Yating
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Department of Pharmacy, No. 309 Hospital of Chinese PLA, Beijing 100091, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Meng
authors.affiliation: Department of Pharmacology and Toxicology, Xi'an Medical University, Xi'an 710021, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Pharmacology and Toxicology, Xi'an Medical University, Xi'an 710021, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Pharmacology and Toxicology, Xi'an Medical University, Xi'an 710021, China.
authors.forename: Jurong
authors.initials: J
authors.lastname: Zeng
mesh_terms: D000107:Acetylation / Q000187:drug effects; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D050882:CREB-Binding Protein / Q000378:metabolism*; D019938:Cyclin D1 / Q000378:metabolism; D019927:Cyclin E / Q000378:metabolism; D058065:Diabetic Cardiomyopathies / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D005434:Flow Cytometry; D007004:Hypoglycemic Agents / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology*; D000072200:Ku Autoantigen / Q000378:metabolism; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D012643:Selenium / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D051028:bcl-2-Associated X Protein / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D019927:Cyclin E; D007004:Hypoglycemic Agents; D007328:Insulin; D051028:bcl-2-Associated X Protein; D019938:Cyclin D1; D050882:CREB-Binding Protein; D048051:p38 Mitogen-Activated Protein Kinases; D000072200:Ku Autoantigen; D012643:Selenium