title: A novel aceE mutation leading to a better growth profile and a higher L-serine production in a high-yield L-serine-producing Corynebacterium glutamicum strain.
abstract: A comparative genomic analysis was performed to study the genetic variations between the L-serine-producing strain Corynebacterium glutamicum SYPS-062 and the mutant strain SYPS-062-33a, which was derived from SYPS-062 by random mutagenesis with enhanced L-serine production. Some variant genes between the two strains were reversely mutated or deleted in the genome of SYPS-062-33a to verify the influences of the gene mutations introduced by random mutagenesis. It was found that a His-594 → Tyr mutation in aceE was responsible for the more accumulation of by-products, such as L-alanine and L-valine, in SYPS-062-33a. Furthermore, the influence of this point mutation on the L-serine production was investigated, and the results suggested that this point mutation led to a better growth profile and a higher L-serine production in the high-yield strain 33a∆SSAAI, which was derived from SYPS-062-33a by metabolic engineering with the highest L-serine production to date.
authors.affiliation: Laboratory of Pharmaceutical Engineering, School of Pharmaceutics Science, Jiangnan University, Wuxi, 214122, People's Republic of China.|The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, People's Republic of China.
authors.forename: Wen
authors.initials: W
authors.lastname: Guo
authors.affiliation: Laboratory of Pharmaceutical Engineering, School of Pharmaceutics Science, Jiangnan University, Wuxi, 214122, People's Republic of China.|The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, People's Republic of China.
authors.forename: Ziwei
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Laboratory of Pharmaceutical Engineering, School of Pharmaceutics Science, Jiangnan University, Wuxi, 214122, People's Republic of China.|The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, People's Republic of China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Laboratory of Pharmaceutical Engineering, School of Pharmaceutics Science, Jiangnan University, Wuxi, 214122, People's Republic of China.|National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, 214122, People's Republic of China.
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Xu
authors.affiliation: Laboratory of Pharmaceutical Engineering, School of Pharmaceutics Science, Jiangnan University, Wuxi, 214122, People's Republic of China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Laboratory of Pharmaceutical Engineering, School of Pharmaceutics Science, Jiangnan University, Wuxi, 214122, People's Republic of China.|The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, People's Republic of China.
authors.forename: Jinsong
authors.initials: J
authors.lastname: Shi
authors.affiliation: Laboratory of Pharmaceutical Engineering, School of Pharmaceutics Science, Jiangnan University, Wuxi, 214122, People's Republic of China. zhenghxu@jiangnan.edu.cn.|The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, People's Republic of China. zhenghxu@jiangnan.edu.cn.
authors.forename: Zhenghong
authors.initials: Z
authors.lastname: Xu
mesh_terms: D048230:Corynebacterium glutamicum / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D014644:Genetic Variation; D023281:Genomics; D060847:Metabolic Engineering; D016296:Mutagenesis; D017354:Point Mutation; D011768:Pyruvate Dehydrogenase Complex / Q000235:genetics*; D012694:Serine / Q000378:metabolism*
chemical_list: D011768:Pyruvate Dehydrogenase Complex; D012694:Serine
keywords: Corynebacterium glutamicum; Genome comparison; L-Serine; Pyruvate dehydrogenase; aceE