title: Hematopoietic, Mesenchymal, and Immune Cells Are More Enhanced in Bone Marrow than in Peripheral Blood from Granulocyte Colony-Stimulating Factor Primed Healthy Donors.
abstract: The use of granulocyte colony-stimulating factor (G-CSF) primed bone marrow (G-BM) has been recently considered as an alternative to mobilized hematopoietic stem cells from peripheral blood (G-PB), especially in the haploidentical transplant setting. The purpose of this study was to compare the effect of in vivo G-CSF priming on BM and PB hematopoietic, mesenchymal (MSC), and immune cells. Forty healthy donors undergoing BM harvest for haploidentical transplant were given subcutaneous recombinant human G-CSF for 7 days. BM and PB samples were harvested on days -7 and 0. The hematopoietic stem/progenitor cells increased significantly after G-CSF priming in both BM and PB with a selective rise of BM CD34(+)CD38(-) cell subset. A striking enhancement of the mesenchymal progenitors was detected in G-BM. CD3(+), CD4(+), CD8(+), and CD19(+) cell fractions; the naive CD4(+) and CD8(+) subpopulations; and natural killer and regulatory T cells increased in G-BM, whereas only slight changes were detected in PB. Myeloid dendritic cells (DC1) were significantly up-regulated in both G-BM and G-PB, whereas DC2 increased only in G-BM. In conclusion, our results show substantial differences in the biologic effects exerted by G-CSF at BM and PB levels on hematopoietic cells and immune cell fractions. Furthermore, the impressive rise of MSC progenitors in G-BM might also be relevant to provide MSCs for several clinical use.
authors.affiliation: Department of Molecular Medicine, "Sapienza" University, Rome, Italy. Electronic address: lidia.defelice@uniroma1.it.
authors.forename: Lidia
authors.initials: L
authors.lastname: De Felice
authors.affiliation: Rome Transplant Network, Stem Cell Transplant Unit, "Tor Vergata" University, Rome, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Agostini
authors.affiliation: Rome Transplant Network, Stem Cell Transplant Unit, "Tor Vergata" University, Rome, Italy.
authors.forename: Carmela
authors.initials: C
authors.lastname: Suriano
authors.affiliation: Rome Transplant Network, Stem Cell Transplant Unit, "Tor Vergata" University, Rome, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Fraboni
authors.affiliation: Hematology Stem Cell Transplant Transfusion Medicine and Cellular Therapy, Campus Bio-Medico University Hospital, Rome, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Gregorj
authors.affiliation: Hematology Stem Cell Transplant Transfusion Medicine and Cellular Therapy, Campus Bio-Medico University Hospital, Rome, Italy.
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Tirindelli
authors.affiliation: Rome Transplant Network, Stem Cell Transplant Unit, "Tor Vergata" University, Rome, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Picardi
authors.affiliation: Bone Marrow Transplant Center, Department of Hematology, Spirito Santo Hospital, Pescara, Italy.
authors.forename: Stella
authors.initials: S
authors.lastname: Santarone
authors.affiliation: Rome Transplant Network, Stem Cell Transplant Unit, "Tor Vergata" University, Rome, Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Di Piazza
authors.affiliation: Bone Marrow Transplant Center, Department of Hematology, Spirito Santo Hospital, Pescara, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Di Bartolomeo
authors.affiliation: Rome Transplant Network, Stem Cell Transplant Unit, "Tor Vergata" University, Rome, Italy.
authors.forename: William
authors.initials: W
authors.lastname: Arcese
mesh_terms: D000328:Adult; D000368:Aged; D001853:Bone Marrow / Q000187:drug effects*; D003713:Dendritic Cells / Q000187:drug effects; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D064368:Healthy Volunteers; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D006801:Humans; D007107:Immune System / Q000166:cytology / Q000187:drug effects; D007694:Killer Cells, Natural / Q000187:drug effects; D008297:Male; D059630:Mesenchymal Stem Cells / Q000187:drug effects; D008875:Middle Aged; D000072916:Peripheral Blood Stem Cells / Q000187:drug effects*; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects; D014019:Tissue Donors
chemical_list: D016179:Granulocyte Colony-Stimulating Factor
keywords: Granulocyte colony-stimulating factor; Haploidentical transplant; Hematopoietic stem cells; Immune modulation; Mesenchymal stem cells