title: Inhibition of green tea polyphenol EGCG((-)-epigallocatechin-3-gallate) on the proliferation of gastric cancer cells by suppressing canonical wnt/β-catenin signalling pathway.
abstract: (-)-Epigallocatechin-3-gallate (EGCG), the major polyphenol in green tea, could affect carcinogenesis and development of many cancers. However, the effects and underlying mechanisms of EGCG on gastric cancer remain unclear. We found that EGCG significantly inhibited proliferation and increased apoptosis of SGC-7901 cells in vitro. The decreased expressions of p-β-catenin(Ser552), p-GSK3β(S9) and β-catenin target genes were detected in SGC-7901 cells after treated by EGCG. XAV939 and β-catenin plasmid were further used to demonstrate the inhibition of EGCG on canonical Wnt/β-catenin signalling. Moreover, EGCG significantly inhibited gastric tumour growth in vivo by inhibiting Wnt/β-catenin signalling. Taken together, our findings establish that EGCG suppressed gastric cancer cell proliferation and demonstrate that this inhibitory effect is related to canonical Wnt/β-catenin signalling. This study raises a new insight into gastric cancer prevention and therapy, and provides evidence that green tea could be used as a nutraceutical beverage.
authors.affiliation: a Department of Gastrointestinal Surgery , Liaocheng People's Hospital , Liaocheng , Shandong , China.
authors.forename: Chenggang
authors.initials: C
authors.lastname: Yang
authors.affiliation: a Department of Gastrointestinal Surgery , Liaocheng People's Hospital , Liaocheng , Shandong , China.
authors.forename: Wenfeng
authors.initials: W
authors.lastname: Du
authors.affiliation: a Department of Gastrointestinal Surgery , Liaocheng People's Hospital , Liaocheng , Shandong , China.
authors.forename: Daogui
authors.initials: D
authors.lastname: Yang
mesh_terms: D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D015398:Signal Transduction; D013274:Stomach Neoplasms / Q000473:pathology*; D013662:Tea / Q000737:chemistry*; D060449:Wnt Signaling Pathway / Q000187:drug effects*; D023041:Xenograft Model Antitumor Assays; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism
chemical_list: D016588:Anticarcinogenic Agents; D013662:Tea; D051176:beta Catenin; D002392:Catechin; C045651:epigallocatechin gallate
keywords: Epigallocatechin-3-gallate; Wnt/β-catenin signalling; gastric carcinoma; proliferation