title: HSV-1 tegument protein and the development of its genome editing technology.
abstract: Herpes simplex virus 1 (HSV-1) is composed of complex structures primarily characterized by four elements: the nucleus, capsid, tegument and envelope. The tegument is an important viral component mainly distributed in the spaces between the capsid and the envelope. The development of viral genome editing technologies, such as the identification of temperature-sensitive mutations, homologous recombination, bacterial artificial chromosome, and the CRISPR/Cas9 system, has been shown to largely contribute to the rapid promotion of studies on the HSV-1 tegument protein. Many researches have demonstrated that tegument proteins play crucial roles in viral gene regulatory transcription, viral replication and virulence, viral assembly and even the interaction of the virus with the host immune system. This article briefly reviews the recent research on the functions of tegument proteins and specifically elucidates the function of tegument proteins in viral infection, and then emphasizes the significance of using genome editing technology in studies of providing new techniques and insights into further studies of HSV-1 infection in the future.
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences, Peking Union Medical College, Kunming, Yunnan, China.
authors.forename: Xingli
authors.initials: X
authors.lastname: Xu
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences, Peking Union Medical College, Kunming, Yunnan, China.
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Che
authors.affiliation: Institute of Medical Biology, Chinese Academy of Medical Sciences, Peking Union Medical College, Kunming, Yunnan, China. liqihan@imbcams.com.cn.
authors.forename: Qihan
authors.initials: Q
authors.lastname: Li
mesh_terms: D000818:Animals; D000072669:Gene Editing; D005821:Genetic Techniques / Q000639:trends; D016679:Genome, Viral; D006561:Herpes Simplex / Q000821:virology*; D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D015678:Viral Structural Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D015678:Viral Structural Proteins
keywords: BAC; CRISPR/Cas9 system; HSV-1; Homologous recombination; Tegument protein