title: Generation and characterization of human iPSC line generated from mesenchymal stem cells derived from adipose tissue.
abstract: In this work, mesenchymal stem cells derived from adipose tissue (ADSCs) were used for the generation of the human-induced pluripotent stem cell line G15.AO. Cell reprogramming was performed using retroviral vectors containing the Yamanaka factors, and the generated G15.AO hiPSC line showed normal karyotype, silencing of the exogenous reprogramming factors, induction of the typical pluripotency-associated markers, alkaline phosphatase enzymatic activity, and in vivo and in vitro differentiation ability to the three germ layers.
authors.affiliation: Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Instituto de Investigación Sanitaria de Navarra, Pamplona, Spain.
authors.forename: Natalia
authors.initials: N
authors.lastname: Zapata-Linares
authors.affiliation: Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Instituto de Investigación Sanitaria de Navarra, Pamplona, Spain.
authors.forename: Saray
authors.initials: S
authors.lastname: Rodriguez
authors.affiliation: Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Instituto de Investigación Sanitaria de Navarra, Pamplona, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Mazo
authors.affiliation: Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Instituto de Investigación Sanitaria de Navarra, Pamplona, Spain.
authors.forename: Gloria
authors.initials: G
authors.lastname: Abizanda
authors.affiliation: Cell Therapy Area, Clínica Universidad de Navarra, University of Navarra, Instituto de Investigación Sanitaria de Navarra, Pamplona, Spain.
authors.forename: Enrique J
authors.initials: EJ
authors.lastname: Andreu
authors.affiliation: Cell Therapy Area, Clínica Universidad de Navarra, University of Navarra, Instituto de Investigación Sanitaria de Navarra, Pamplona, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: Barajas
authors.affiliation: Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Instituto de Investigación Sanitaria de Navarra, Pamplona, Spain; Cell Therapy Area, Clínica Universidad de Navarra, University of Navarra, Instituto de Investigación Sanitaria de Navarra, Pamplona, Spain. Electronic address: fprosper@unav.es.
authors.forename: Felipe
authors.initials: F
authors.lastname: Prosper
authors.affiliation: Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Instituto de Investigación Sanitaria de Navarra, Pamplona, Spain. Electronic address: jrrodriguez@unav.es.
authors.forename: Juan R
authors.initials: JR
authors.lastname: Rodriguez-Madoz
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D055028:Comparative Genomic Hybridization; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D007621:Karyotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology*