title: Lanostane triterpenoids from Ganoderma curtisii and their NO production inhibitory activities of LPS-induced microglia.
abstract: Twenty-nine lanostane triterpenoids (1-29) were obtained from the EtOH extract of fruiting bodies of the Ganoderma curtisii. Among them, compound 1 was a new lanostane triterpenoid and compounds 2-5 were isolated from the genus Ganoderma for the first time and their structures were unambiguously identified in this work. The NMR data of the four known lanostane triterpenoids (2-5) were reported for the first time because their structures were all tentatively characterized by interpreting the MS data from the methanol extract of Ganoderma lucidum or from the metabolites in rat bile after oral administration of crude extract of the fruiting bodies of G. lucidum using fragmentation rules. Their anti-inflammatory activities were tested by measuring their inhibitory effects on nitric oxide (NO) production in BV-2 microglia cells activated by lipopolysaccharide. Their IC50 values were in a range from 3.65±0.41 to 28.04±2.81μM.
authors.affiliation: School of Pharmaceutical Science, Guangxi Medical University, Nanning 530021, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Jiao
authors.affiliation: School of Pharmaceutical Science, Guangxi Medical University, Nanning 530021, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Xie
authors.affiliation: School of Pharmaceutical Science, Guangxi Medical University, Nanning 530021, China.
authors.forename: Lu-Hui
authors.initials: LH
authors.lastname: Zou
authors.affiliation: School of Pharmaceutical Science, Guangxi Medical University, Nanning 530021, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wei
authors.affiliation: School of Pharmaceutical Science, Guangxi Medical University, Nanning 530021, China. Electronic address: qlydmyyq@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Qiu
authors.affiliation: Department of Natural Products Chemistry, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China; Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China. Electronic address: syzyclx@163.com.
authors.forename: Li-Xia
authors.initials: LX
authors.lastname: Chen
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D032902:Ganoderma / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D051379:Mice; D017628:Microglia / Q000187:drug effects*; D015394:Molecular Structure; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D051381:Rats; D013329:Structure-Activity Relationship; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D008070:Lipopolysaccharides; D014315:Triterpenes; D009569:Nitric Oxide
keywords: BV-2 microglia cell; Ganoderma curtisii; Lanostane triterpenoids; NO production inhibitory activity