title: Identification of genes showing differential expression profile associated with growth rate in skeletal muscle tissue of Landrace weanling pig.
abstract: Suppression subtractive hybridization was used to identify genes showing differential expression profile associated with growth rate in skeletal muscle tissue of Landrace weanling pig. Two subtracted cDNA populations were generated from musculus longissimus muscle tissues of selected pigs with extreme expected breeding values at the age of 100 kg. Three upregulated genes (EEF1A2, TSG101 and TTN) and six downregulated genes (ATP5B, ATP5C1, COQ3, HADHA, MYH1 and MYH7) in pig with genetic propensity for higher growth rate were identified by sequence analysis of 12 differentially expressed clones selected by differential screening following the generation of the subtracted cDNA population. Real-time PCR analysis confirmed difference in expression profiles of the identified genes in musculus longissimus muscle tissues between the two Landrace weanling pig groups with divergent genetic propensity for growth rate. Further, differential expression of the identified genes except for the TTN was validated by Western blot analysis. Additionally, the eight genes other than the ATP5C1 colocalized with the same chromosomal positions as QTLs that have been previously identified for growth rate traits. Finally, the changes of expression predicted from gene function suggested association of upregulation of expression of the EEF1A2, TSG101 and TTN genes and downregulation of the ATP5B, ATP5C1, COQ3, HADHA, MYH1 and MYH7 gene expression with increased growth rate. The identified genes will provide an important insight in understanding the molecular mechanism underlying growth rate in Landrace pig breed.
authors.affiliation: Faculty of Agriculture, Department of Agrobiology, Niigata University, Nishi-ku, Niigata 950-2181, Japan.tyamada@agr.niigata-u.ac.jp.
authors.forename: Yuuta
authors.initials: Y
authors.lastname: Komatsu
authors.forename: Shin
authors.initials: S
authors.lastname: Sukegawa
authors.forename: Mai
authors.initials: M
authors.lastname: Yamashita
authors.forename: Naoki
authors.initials: N
authors.lastname: Katsuda
authors.forename: Bin
authors.initials: B
authors.lastname: Tong
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ohta
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kose
authors.forename: Takahisa
authors.initials: T
authors.lastname: Yamada
mesh_terms: D000818:Animals; D018507:Gene Expression Regulation, Developmental; D008297:Male; D008460:Meat; D024510:Muscle Development / Q000235:genetics*; D009124:Muscle Proteins / Q000235:genetics* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000254:growth & development / Q000378:metabolism; D010641:Phenotype; D040641:Quantitative Trait Loci; D019655:Quantitative Trait, Heritable; D066251:Subtractive Hybridization Techniques; D013552:Swine; D014886:Weaning; D015430:Weight Gain
chemical_list: D009124:Muscle Proteins