title: Quantitative and compositional responses of ammonia-oxidizing archaea and bacteria to long-term field fertilization.
abstract: Archaeal (AOA) and bacterial (AOB) ammonia-oxidizer responses to long-term field fertilization in a Mollisol soil were assessed through pyrosequencing of amoA genes. Long-term fertilization treatments including chemical fertilizer (NPK), NPK plus manure (NPKM), and no fertilization over 23 years altered soil properties resulting in significant shifts in AOA and AOB community composition and abundance. NPK exhibited a strong influence on AOA and AOB composition while the addition of manure neutralized the community change induced by NPK. NPK also led to significant soil acidification and enrichment of Nitrosotalea. Nitrosospira cluster 9 and 3c were the most abundant AOB populations with opposing responses to fertilization treatments. NPKM had the largest abundance of ammonia-oxidizers and highest potential nitrification activity (PNA), suggesting high N loss potential due to a doubling of nutrient input compared to NPK. PNA was strongly correlated to AOA and AOB community composition indicating that both were important in ammonium oxidization in this Mollisol soil. Total N and organic C were the most important factors driving shifts in AOA and AOB community composition. The AOA community was strongly correlated to the activities of all sugar hydrolysis associated soil enzymes and was more responsive to C and N input than AOB.
authors.affiliation: Jiangsu Collaborative Innovation Center for Solid Organic Waste Utilization and National Engineering Research Center for Organic-based Fertilizers, Department of Plant Nutrition, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Xue
authors.affiliation: Jiangsu Collaborative Innovation Center for Solid Organic Waste Utilization and National Engineering Research Center for Organic-based Fertilizers, Department of Plant Nutrition, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Jiangsu Collaborative Innovation Center for Solid Organic Waste Utilization and National Engineering Research Center for Organic-based Fertilizers, Department of Plant Nutrition, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhu
authors.affiliation: Jiangsu Collaborative Innovation Center for Solid Organic Waste Utilization and National Engineering Research Center for Organic-based Fertilizers, Department of Plant Nutrition, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Agriculture Environment and Resources Center, Jilin Academy of Agricultural Sciences, Jilin 130033, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhu
authors.affiliation: Agriculture Environment and Resources Center, Jilin Academy of Agricultural Sciences, Jilin 130033, China.
authors.forename: Chang
authors.initials: C
authors.lastname: Peng
authors.affiliation: Jiangsu Collaborative Innovation Center for Solid Organic Waste Utilization and National Engineering Research Center for Organic-based Fertilizers, Department of Plant Nutrition, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Ling
authors.affiliation: Jiangsu Collaborative Innovation Center for Solid Organic Waste Utilization and National Engineering Research Center for Organic-based Fertilizers, Department of Plant Nutrition, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China.
authors.forename: Qirong
authors.initials: Q
authors.lastname: Shen
mesh_terms: D000383:Agriculture / Q000379:methods*; D000641:Ammonia / Q000378:metabolism*; D001105:Archaea / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D058448:Biota / Q000187:drug effects*; D005308:Fertilizers / Q000706:statistics & numerical data; D019766:Genes, Archaeal; D005798:Genes, Bacterial; D010084:Oxidation-Reduction; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D005308:Fertilizers; D000641:Ammonia