title: Effect of glucose on Listeria monocytogenes biofilm formation, and assessment of the biofilm's sanitation tolerance.
abstract: Listeria monocytogenes is an important cause of human foodborne infections and its ability to form biofilms is a serious concern to the food industry. To reveal the effect of glucose conditions on biofilm formation of L. monocytogenes, 20 strains were investigated under three glucose conditions (0.1, 1.0, and 2.0% w v(-1)) by quantifying the number of cells in the biofilm and observing the biofilm structure after incubation for 24, 72, and 168 h. In addition, the biofilms were examined for their sensitivity to sodium hypochlorite. It was found that high concentrations of glucose reduced the number of viable cells in the biofilms and increased extracellular polymeric substance production. Moreover, biofilms formed at a glucose concentration of 1.0 or 2.0% were more resistant to sodium hypochlorite than those formed at a glucose concentration of 0.1%. This knowledge can be used to help design the most appropriate sanitation strategy.
authors.affiliation: a Department of Food Science and Technology, Faculty of Marine Science , Tokyo University of Marine Science and Technology , Tokyo , Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kyoui
authors.affiliation: a Department of Food Science and Technology, Faculty of Marine Science , Tokyo University of Marine Science and Technology , Tokyo , Japan.
authors.forename: Eri
authors.initials: E
authors.lastname: Hirokawa
authors.affiliation: a Department of Food Science and Technology, Faculty of Marine Science , Tokyo University of Marine Science and Technology , Tokyo , Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Takahashi
authors.affiliation: a Department of Food Science and Technology, Faculty of Marine Science , Tokyo University of Marine Science and Technology , Tokyo , Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kuda
authors.affiliation: a Department of Food Science and Technology, Faculty of Marine Science , Tokyo University of Marine Science and Technology , Tokyo , Japan.
authors.forename: Bon
authors.initials: B
authors.lastname: Kimura
mesh_terms: D058491:Bacterial Load; D018441:Biofilms / Q000187:drug effects*; D024881:Drug Resistance, Bacterial; D005525:Food-Processing Industry; D005947:Glucose / Q000008:administration & dosage*; D006801:Humans; D008089:Listeria monocytogenes / Q000187:drug effects / Q000502:physiology*; D018625:Microscopy, Atomic Force; D012499:Sanitation; D012973:Sodium Hypochlorite / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D012973:Sodium Hypochlorite; D005947:Glucose
keywords: Listeria monocytogenes; atomic force microscopy; biofilm; extracellular polymeric substance; glucose; sanitation tolerance