title: Heparin micropatterning onto fouling-release perfluoropolyether-based polymers via photobiotin activation.
abstract: A simple method for constructing versatile ordered biotin/avidin arrays on UV-curable perfluoropolyethers (PFPEs) is presented. The goal is the realization of a versatile platform where any biotinylated biological ligands can be further linked to the underlying biotin/avidin array. To this end, microcontact arrayer and microcontact printing technologies were developed for photobiotin direct printing on PFPEs. As attested by fluorescence images, we demonstrate that this photoactive form of biotin is capable of grafting onto PFPEs surfaces during irradiation. Bioaffinity conjugation of the biotin/avidin system was subsequently exploited for further self-assembly avidin family proteins onto photobiotin arrays. The excellent fouling release PFPEs surface properties enable performing avidin assembly step simply by arrays incubation without PFPEs surface passivation or chemical modification to avoid unspecific biomolecule adsorption. Finally, as a proof of principle biotinylated heparin was successfully grafted onto photobiotin/avidin arrays.
authors.affiliation: Dipartimento di Chimica, Materiali e Ingegneria Chimica "Giulio Natta", Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano, Italy. Electronic address: caterina.credi@polimi.it.
authors.forename: Caterina
authors.initials: C
authors.lastname: Credi
authors.affiliation: Dipartimento di Chimica, Materiali e Ingegneria Chimica "Giulio Natta", Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano, Italy.
authors.forename: Carmela
authors.initials: C
authors.lastname: De Marco
authors.affiliation: Dipartimento di Chimica, Materiali e Ingegneria Chimica "Giulio Natta", Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Molena
authors.affiliation: Nanobioengineering group, Institute for Bioengineering of Catalonia (IBEC), Baldiri-Reixac 10-12, 08028 Barcelona, Spain.
authors.forename: Mateu
authors.initials: M
authors.lastname: Pla Roca
authors.affiliation: Nanobioengineering group, Institute for Bioengineering of Catalonia (IBEC), Baldiri-Reixac 10-12, 08028 Barcelona, Spain; The Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Maria de Luna, 11, 50018, Zaragoza, Spain; Department of Electronics, University of Barcelona (UB), Martí i Franquès, 1, Barcelona 08028, Spain.
authors.forename: Josep
authors.initials: J
authors.lastname: Samitier Martí
authors.affiliation: Barcelona Institute for Global Health (ISGlobal, Hospital Clínic-Universitat de Barcelona), Rosselló 149-153, 08036 Barcelona, Spain; Nanoscience and Nanotechnology Institute (IN2UB), University of Barcelona, Martí i Franquès 1, 08028 Barcelona, Spain; Nanomalaria Group, Institute for Bioengineering of Catalonia (IBEC), Baldiri-Reixac 10-12, 08028 Barcelona, Spain.
authors.forename: Joana
authors.initials: J
authors.lastname: Marques
authors.affiliation: Barcelona Institute for Global Health (ISGlobal, Hospital Clínic-Universitat de Barcelona), Rosselló 149-153, 08036 Barcelona, Spain; Nanoscience and Nanotechnology Institute (IN2UB), University of Barcelona, Martí i Franquès 1, 08028 Barcelona, Spain; Nanomalaria Group, Institute for Bioengineering of Catalonia (IBEC), Baldiri-Reixac 10-12, 08028 Barcelona, Spain.
authors.forename: Xavier
authors.initials: X
authors.lastname: Fernàndez-Busquets
authors.affiliation: Dipartimento di Chimica, Materiali e Ingegneria Chimica "Giulio Natta", Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano, Italy.
authors.forename: Marinella
authors.initials: M
authors.lastname: Levi
authors.affiliation: Dipartimento di Chimica, Materiali e Ingegneria Chimica "Giulio Natta", Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Turri
mesh_terms: D001360:Avidin / Q000737:chemistry*; D001386:Azides / Q000737:chemistry*; D057219:Biofouling; D001710:Biotin / Q000031:analogs & derivatives* / Q000737:chemistry; D019921:Biotinylation; D004987:Ethers / Q000737:chemistry*; D005453:Fluorescence; D005466:Fluorocarbons / Q000737:chemistry*; D006493:Heparin / Q000737:chemistry*; D055668:Photochemical Processes; D011108:Polymers / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D001386:Azides; D004987:Ethers; D005466:Fluorocarbons; D011108:Polymers; C078113:perfluoropolyether; D001360:Avidin; D001710:Biotin; D006493:Heparin; C045549:photobiotin
keywords: Antifouling; Heparin; Malaria; Microcontact arrayer; Microcontact printing; Micropatterning; Perfluoropolyether; Photobiotin; Polymers; Soft lithography