title: miR-376c inhibits cervical cancer cell proliferation and invasion by targeting BMI1.
abstract: MicroRNAs (miRNAs) play crucial roles in cancer development and progression. The purposes of this study were to explore the role of miR-376c in cervical cancer and to clarify the regulation of BMI1 by miR-376c. Quantitative RT-PCR was used to measure miR-376c expression in cervical cancer tissues and cell lines. The cell proliferation, cell cycle and Transwell invasion assays were performed. A luciferase reporter assay was conducted to confirm the target gene of miR-376c, and the results were validated in cervical cancer cell lines and tissues. MiR-376c was significantly downregulated in cervical cancer cell lines and clinical tissues. Upregulation of miR-376c impaired cell proliferation, blocked G1/S checkpoint of cell cycle and suppressed cell invasion in vitro. BMI1 was verified as a direct target of miR-376c, which was further confirmed by the inverse expression of miR-376c and BMI1 in patient specimens. The newly identified miR-376c/BMI1 pathway provides an insight into cervical cancer progression and may represent a novel therapeutic target.
authors.affiliation: Department of Pediatrics, Zhongnan Hospital of Wuhan University, Wuhan, China.
authors.forename: Youping
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Department of gynaecology oncology, Zhongnan Hospital of Wuhan University, Wuhan, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: Medical Research Center, Zhongnan Hospital of Wuhan University, Wuhan, China.
authors.forename: Yingjuan
authors.initials: Y
authors.lastname: Liu
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000235:genetics*; D063150:Polycomb Repressive Complex 1 / Q000378:metabolism*; D015854:Up-Regulation; D002583:Uterine Cervical Neoplasms / Q000235:genetics*
chemical_list: C069077:BMI1 protein, human; C573498:MIRN376C microRNA, human; D035683:MicroRNAs; D063150:Polycomb Repressive Complex 1
keywords: BMI1; Cervical cancer; HPV; miR-376c