title: Two types of soybean diacylglycerol acyltransferases are differentially involved in triacylglycerol biosynthesis and response to environmental stresses and hormones.
abstract: Diacylglycerol acyltransferases (DGATs) play a key role in plant triacylglycerol (TAG) biosynthesis. Two type 1 and 2 DGATs from soybean were characterized for their functions in TAG biosynthesis and physiological roles. GmDGAT1A is highly expressed in seeds while GmDGAT2D is mainly expressed in flower tissues. They showed different expression patterns in response to biotic and abiotic stresses. GmDGAT2D was up-regulated by cold and heat stress and ABA signaling, and repressed by insect biting and jasmonate, whereas GmDGAT1A show fewer responses. Both GmDGAT1A and GmDGAT2D were localized to the endoplasmic reticulum and complemented the TAG deficiency of a yeast mutant H1246. GmDGAT2D-transgenic hairy roots synthesized more 18:2- or 18:1-TAG, whereas GmDGAT1A prefers to use 18:3-acyl CoA for TAG synthesis. Overexpression of both GmDGATs in Arabidopsis seeds enhanced the TAG production; GmDGAT2D promoted 18:2-TAG in wild-type but enhanced 18:1-TAG production in rod1 mutant seeds, with a decreased 18:3-TAG. However, GmDGAT1A enhanced 18:3-TAG and reduced 20:1-TAG contents. The different substrate preferences of two DGATs may confer diverse fatty acid profiles in soybean oils. While GmDGAT1A may play a role in usual seed TAG production and GmDGAT2D is also involved in usual TAG biosynthesis in other tissues in responses to environmental and hormonal cues.
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, College of Plant Science &Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: BeiBei
authors.initials: B
authors.lastname: Chen
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, College of Plant Science &Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Junejie
authors.initials: J
authors.lastname: Wang
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, College of Plant Science &Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Gaoyang
authors.initials: G
authors.lastname: Zhang
authors.affiliation: College of Agronomy, Jilin Agricultural University, Changchun, 130047, China.
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Liu
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, College of Plant Science &Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Sehrish
authors.initials: S
authors.lastname: Manan
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, College of Life Science &Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Honghong
authors.initials: H
authors.lastname: Hu
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, College of Plant Science &Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhao
mesh_terms: D000214:Acyl Coenzyme A / Q000378:metabolism; D017360:Arabidopsis / Q000378:metabolism; D051048:Diacylglycerol O-Acyltransferase / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D035264:Flowers / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000502:physiology; D006728:Hormones / Q000378:metabolism*; D012639:Seeds / Q000378:metabolism; D013024:Soybean Oil / Q000378:metabolism; D013025:Glycine max / Q000378:metabolism*; D013312:Stress, Physiological / Q000502:physiology*; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D000214:Acyl Coenzyme A; D005227:Fatty Acids; D006728:Hormones; D014280:Triglycerides; D013024:Soybean Oil; D051048:Diacylglycerol O-Acyltransferase