title: Exogenous Magnesium Chloride Reduces the Activated Partial Thromboplastin Times of Lupus Anticoagulant-Positive Patients.
abstract: The activated partial thromboplastin time (APTT) assay is a basic hemostatic assay based on the time it takes for clots to form in plasma samples after the addition of calcium chloride. It is used to screen for various coagulation disorders. Several previous reports have suggested that magnesium (Mg) might contribute to coagulation reactions by binding to specific coagulation proteins. We investigated the effects of Mg on the APTT. In healthy controls, the APTT was significantly prolonged in proportion to the increase in the concentration of magnesium chloride in the range from 2.1 to 16.7 mmol/L. Among eight samples from patients with various disorders that exhibited prolonged APTT, two samples demonstrated shorter APTT when Mg was added, both of which were from patients that were positive for lupus anticoagulant. When we examined 206 clinical APTT samples, we found that Mg shortened the APTT of two samples. These two samples were also from lupus anticoagulant-positive patients (p-value: <0.003). Our findings regarding the unique effects of exogenous Mg on the APTT of lupus anticoagulant-positive patients might shed light on the role of Mg in APTT assays and lead to the development of a novel screening method for lupus anticoagulant.
authors.affiliation: Department of Clinical Laboratory, Nagano Red Cross Hospital, Nagano, Japan.
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Tokutake
authors.affiliation: Department of Clinical Laboratory, Nagano Red Cross Hospital, Nagano, Japan.
authors.forename: Hisami
authors.initials: H
authors.lastname: Baba
authors.affiliation: Department of Clinical Laboratory, Nagano Red Cross Hospital, Nagano, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Shimada
authors.affiliation: Department of Hematology, Nagano Red Cross Hospital, Nagano, Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Takeda
authors.affiliation: Department of Hematology, Nagano Red Cross Hospital, Nagano, Japan.
authors.forename: Keijiro
authors.initials: K
authors.lastname: Sato
authors.affiliation: Department of Hematology, Nagano Red Cross Hospital, Nagano, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Hiroshima
authors.affiliation: Department of Hematology, Nagano Red Cross Hospital, Nagano, Japan.
authors.forename: Takehiko
authors.initials: T
authors.lastname: Kirihara
authors.affiliation: Department of Hematology, Nagano Red Cross Hospital, Nagano, Japan.|Center for Medical Education, Shinshu University School of Medicine, Matsumoto, Japan.
authors.forename: Ikuo
authors.initials: I
authors.lastname: Shimizu
authors.affiliation: Department of Hematology, Shinshu University School of Medicine, Matsumoto, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Nakazawa
authors.affiliation: Department of Clinical Laboratory, Nagano Red Cross Hospital, Nagano, Japan.|Department of Hematology, Nagano Red Cross Hospital, Nagano, Japan.
authors.forename: Hikaru
authors.initials: H
authors.lastname: Kobayashi
authors.affiliation: Department of Hematology, Shinshu University School of Medicine, Matsumoto, Japan.|Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University School of Medicine, Matsumoto, Japan.
authors.forename: Fumihiro
authors.initials: F
authors.lastname: Ishida
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016736:Antiphospholipid Syndrome / Q000175:diagnosis / Q000276:immunology*; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007477:Ions; D016682:Lupus Coagulation Inhibitor / Q000276:immunology*; D008274:Magnesium / Q000737:chemistry; D015636:Magnesium Chloride / Q000627:therapeutic use*; D008297:Male; D008875:Middle Aged; D010314:Partial Thromboplastin Time
chemical_list: D007477:Ions; D016682:Lupus Coagulation Inhibitor; D015636:Magnesium Chloride; D008274:Magnesium