title: Effect of removing cumulus cells from porcine cumulus-oocyte complexes derived from small and medium follicles during IVM on the apoptotic status and meiotic progression of the oocytes.
abstract: The present study was undertaken to examine the apoptotic status and meiotic progression of oocytes from small follicle (SF; 0.5-2 mm in diameter) and medium follicle (MF; 3-6 mm in diameter) when the oocytes were denuded before, during, and after IVM. Cumulus-oocyte complexes (COCs) were collected from SF or MF of prepubertal gilt ovaries. Before or 20 hours after the start of IVM culture, some oocytes were denuded and cultured for IVM. At the end of IVM, apoptotic status and meiotic progression of the oocytes were compared with oocytes matured in the presence of cumulus cells (CCs) by Annexin-V/PI assay and 4',6-Diamidino-2-phenylindole staining. Apoptotic status of the oocytes was only affected by time when the oocytes were denuded. In both oocytes from SF and MF, although the incidence of early and late apoptotic oocytes was significantly higher when the CCs were removed before IVM, the rate was significantly lower when CCs were removed 20 and 44 hours after the start of IVM. The incidence of mature oocytes was significantly affected by both the origin of COCs and time when oocytes were denuded from the COCs. Although the percentage of mature oocytes was higher in MF than SF, maturation rates were significantly higher when oocytes were denuded 20 hours, as compared with 0 and 44 hours after the start of IVM. However, the percentage of mature oocytes with a morphologically normal spindle was significantly higher when oocytes were denuded 44 hours, rather than 22 hours of IVM. In conclusion, removing CCs 20 hours after the start of IVM seems to promote meiotic progression of the oocytes to the metaphase-II stage even when the COCs were collected from SF, although factor(s) from or communication with CCs during IVM may need to obtain a morphologically normal spindle in mature oocytes.
authors.affiliation: Department of Animal Science, Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan.
authors.forename: Pilar
authors.initials: P
authors.lastname: Ferré
authors.affiliation: Department of Animal Science, Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan.
authors.forename: Tra Mi Thi
authors.initials: TM
authors.lastname: Bui
authors.affiliation: Department of Animal Science, Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Wakai
authors.affiliation: Department of Animal Science, Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan. Electronic address: hirofun@okayama-u.ac.jp.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Funahashi
mesh_terms: D000818:Animals; D002470:Cell Survival; D054885:Cumulus Cells / Q000502:physiology*; D005260:Female; D059471:In Vitro Oocyte Maturation Techniques / Q000662:veterinary*; D008540:Meiosis / Q000502:physiology*; D009865:Oocytes / Q000502:physiology*; D006080:Ovarian Follicle / Q000166:cytology*; D013552:Swine / Q000502:physiology*
keywords: Apoptosis; Cumulus cells; IVM; Oocyte; Prepubertal gilt (pigs)