title: Sensitive Naked Eye and Autofluorescence Detection of Cu(2+) in Biological Fluids by Polyethyleneimine Microspheres.
abstract: In this paper, an autofluorencent polyethyleneimine (PEI) microspheres probe has been designed as the two channel sensor for both quantitative fluorescence and visual detection of Cu(2+). We synthesized autofluorescent microspheres crosslinked with glutaraldehyde by controlling volume ratio between oil phase and water phase. A novel fluorescent platform for sensing Cu(2+) based on the autofluorescence quenching of PEI microspheres by Cu(2+) with a detection limit as low as 2 nM. In addition, the absorbance of PEI microspheres at 205 nm, 325 nm, 590 nm and 800 nm were sensitive to the presence of Cu(2+). The strong chelation interaction between Cu(2+) and -NH2 on the surface of microspheres produced the fluorescence quenching and color change of PEI microspheres which facilitated our sensing approach. We regard the developing of autofluorcent PEI microspheres as the dual-channel sensor for Cu(2+) would provide autofluorcent microspheres a future application in quantitative analysis.
authors.affiliation: Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Yan
authors.affiliation: Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Deng
authors.affiliation: Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, China. heyu@hubu.edu.cn.|Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Hubei University, Wuhan, 430062, China. heyu@hubu.edu.cn.
authors.forename: Yu
authors.identifier: http://orcid.org/0000-0002-8068-5825
authors.initials: Y
authors.lastname: He
authors.affiliation: Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, China.|Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Hubei University, Wuhan, 430062, China.
authors.forename: Yili
authors.initials: Y
authors.lastname: Ge
authors.affiliation: Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, China.|Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Hubei University, Wuhan, 430062, China.
authors.forename: Gongwu
authors.initials: G
authors.lastname: Song
mesh_terms: D003300:Copper / Q000032:analysis* / Q000097:blood / Q000652:urine; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D008863:Microspheres; D011094:Polyethyleneimine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D014867:Water / Q000032:analysis*
chemical_list: D005456:Fluorescent Dyes; D014867:Water; D003300:Copper; D011094:Polyethyleneimine
keywords: Autofluorescent microspheres; Cu2+; Fluorescence quenching; Visual detection