title: Selective plane illumination microscopy with a light sheet of uniform thickness formed by an electrically tunable lens.
abstract: Light sheet microscopy is a powerful technique for rapid, three-dimensional fluorescence imaging of large specimen such as drosophila and zebrafish embryos. Yet, beam divergence results in a loss of axial resolution at the periphery of the light sheet. Here, we demonstrate how an electrically tunable lens can be utilized to maintain the minimal, diffraction-limited thickness of the light sheet over a wide field of view (>600 µm) at high frame rates (40 fps). This mode of operation is necessary for the application of fluorescence fluctuation spectroscopy in images. Microsc. Res. Tech. 81:924-928, 2018. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California, USA.
authors.forename: Per Niklas
authors.initials: PN
authors.lastname: Hedde
authors.affiliation: Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California, USA.
authors.forename: Enrico
authors.initials: E
authors.lastname: Gratton
mesh_terms: D000818:Animals; D004330:Drosophila / Q000033:anatomy & histology; D007909:Lenses; D008029:Lighting / Q000295:instrumentation / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D014466:Ultraviolet Rays; D015027:Zebrafish / Q000033:anatomy & histology
keywords: fluorescence fluctuation spectroscopy; fluorescence microscopy; light sheet microscopy