title: Transient receptor potential vanilloid 4-dependent calcium influx and ATP release in mouse and rat gastric epithelia.
abstract: OBJECTIVE
  To explore the expression of transient receptor potential vanilloid 4 (TRPV4) and its physiological meaning in mouse and rat gastric epithelia.
  
  
  METHODS
  RT-PCR and immunochemistry were used to detect TRPV4 mRNA and protein expression in mouse stomach and a rat normal gastric epithelial cell line (RGE1-01), while Ca(2+)-imaging and electrophysiology were used to evaluate TRPV4 channel activity. ATP release was measured by a luciferin-luciferase assay. Gastric emptying was also compared between WT and TRPV4 knockout mice.
  
  
  RESULTS
  TRPV4 mRNA and protein were detected in mouse tissues and RGE1-01 cells. A TRPV4-specific agonist (GSK1016790A) increased intracellular Ca(2+) concentrations and/or evoked TRPV4-like current activities in WT mouse gastric epithelial cells and RGE1-01 cells, but not TRPV4KO cells. GSK1016790A or mechanical stimuli induced ATP release from RGE1-01 cells while TRPV4 knockout mice displayed delayed gastric emptying in vivo.
  
  
  CONCLUSIONS
  TRPV4 is expressed in mouse and rat gastric epithelium and contributes to ATP release and gastric emptying.
authors.affiliation: Hiroshi Mihara, Nobuhiro Suzuki, Jibran Sualeh Muhammad, Toshiro Sugiyama, Department of Gastroenterology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0194, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mihara
authors.affiliation: Hiroshi Mihara, Nobuhiro Suzuki, Jibran Sualeh Muhammad, Toshiro Sugiyama, Department of Gastroenterology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0194, Japan.
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Suzuki
authors.affiliation: Hiroshi Mihara, Nobuhiro Suzuki, Jibran Sualeh Muhammad, Toshiro Sugiyama, Department of Gastroenterology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0194, Japan.
authors.forename: Ammar Abdullkader
authors.initials: AA
authors.lastname: Boudaka
authors.affiliation: Hiroshi Mihara, Nobuhiro Suzuki, Jibran Sualeh Muhammad, Toshiro Sugiyama, Department of Gastroenterology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0194, Japan.
authors.forename: Jibran Sualeh
authors.initials: JS
authors.lastname: Muhammad
authors.affiliation: Hiroshi Mihara, Nobuhiro Suzuki, Jibran Sualeh Muhammad, Toshiro Sugiyama, Department of Gastroenterology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0194, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Tominaga
authors.affiliation: Hiroshi Mihara, Nobuhiro Suzuki, Jibran Sualeh Muhammad, Toshiro Sugiyama, Department of Gastroenterology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0194, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Tabuchi
authors.affiliation: Hiroshi Mihara, Nobuhiro Suzuki, Jibran Sualeh Muhammad, Toshiro Sugiyama, Department of Gastroenterology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama 930-0194, Japan.
authors.forename: Toshiro
authors.initials: T
authors.lastname: Sugiyama
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism*; D005746:Gastric Emptying; D005753:Gastric Mucosa / Q000378:metabolism*; D007150:Immunohistochemistry; D007930:Leucine / Q000031:analogs & derivatives / Q000494:pharmacology; D051379:Mice; D018345:Mice, Knockout; D018408:Patch-Clamp Techniques; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013449:Sulfonamides / Q000494:pharmacology; D050916:TRPV Cation Channels / Q000819:agonists / Q000235:genetics* / Q000378:metabolism
chemical_list: C530602:N-(1-((4-(2-(((2,4-dichlorophenyl)sulfonyl)amino)-3-hydroxypropanoyl)-1-piperazinyl)carbonyl)-3-methylbutyl)-1-benzothiophene-2-carboxamide; D013449:Sulfonamides; D050916:TRPV Cation Channels; C486576:Trpv4 protein, mouse; C486577:Trpv4 protein, rat; D000255:Adenosine Triphosphate; D007930:Leucine; D002118:Calcium
keywords: ATP; Gastric emptying; Stomach; Transient receptor potential vanilloid 4