title: A molecular beacon-based DNA switch for reversible pH sensing in vesicles and live cells.
abstract: In this Communication, a molecular beacon-based DNA switch (LMB) is developed as an efficient and reversible pH sensing probe. Remarkably, LMB exhibited reversible structural transition between the closed (molecular beacon) and open (A-motif) states very efficiently in synthetic vesicles and live cells without the need for any transfection agents.
authors.affiliation: Bioorganic Chemistry Laboratory, New Chemistry Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur P.O., Bengaluru 560064, India. tgraju@jncasr.ac.in.
authors.forename: Nagarjun
authors.initials: N
authors.lastname: Narayanaswamy
authors.affiliation: Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bengaluru 560012, India.
authors.forename: Raji R
authors.initials: RR
authors.lastname: Nair
authors.affiliation: Bioorganic Chemistry Laboratory, New Chemistry Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur P.O., Bengaluru 560064, India. tgraju@jncasr.ac.in.
authors.forename: Y V
authors.initials: YV
authors.lastname: Suseela
authors.affiliation: Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bengaluru 560012, India.
authors.forename: Deepak Kumar
authors.initials: DK
authors.lastname: Saini
authors.affiliation: Bioorganic Chemistry Laboratory, New Chemistry Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur P.O., Bengaluru 560064, India. tgraju@jncasr.ac.in.
authors.forename: T
authors.initials: T
authors.lastname: Govindaraju
mesh_terms: D002232:Carbocyanines / Q000032:analysis; D002470:Cell Survival; D004247:DNA / Q000737:chemistry*; D031541:Fluorescence Resonance Energy Transfer; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D015335:Molecular Probes / Q000032:analysis* / Q000737:chemistry*
chemical_list: D002232:Carbocyanines; D015335:Molecular Probes; C089187:cyanine dye 3; C085321:cyanine dye 5; D004247:DNA