title: Long noncoding RNA are aberrantly expressed in human papillary thyroid carcinoma.
abstract: Long noncoding RNAs (lncRNAs) have emerged as key regulatory molecules at almost every level of gene expression regulation. The altered expression of lncRNAs is a characteristic of numerous types of cancer, and lncRNAs have been demonstrated to promote the development, invasion and metastasis of tumors through various mechanisms. However, the role of lncRNAs in papillary thyroid carcinoma (PTC) remain unclear. In the present study, differentially expressed lncRNAs and mRNAs were detected by human lncRNA microarray in three pairs of PTC and adjacent noncancerous samples. The microarray results revealed that 675 lncRNAs and 751 mRNAs were abnormally expressed in the three PTC samples compared with adjacent noncancerous samples (fold change ≥2.0; P<0.05). To validate the microarray results, 8 differentially expressed lncRNAs were randomly selected for quantitative polymerase chain reaction (qPCR). The results of qPCR were consistent with the microarray data; the 8 lncRNAs had an aberrant expression in the PTC samples compared with the adjacent noncancerous samples. Gene ontology and pathway analysis indicated that there were 7 downregulated pathways and 29 upregulated pathways in PTC. LncRNA classification and subgroup analysis revealed 7 pairs of enhancer-like lncRNA-mRNA, 9 pairs of antisense lncRNA-mRNA and 45 pairs of lncRNA-mRNA were differentially expressed between PTC and their paired noncancerous samples. In conclusion, the present study identified a series of novel PTC-associated lncRNAs. Further study with these lncRNAs is instrumental for the identification of novel target molecules that could lead to improved diagnosis and treatment for PTC.
authors.affiliation: Department of Internal Medicine, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China.
authors.forename: Meiliu
authors.initials: M
authors.lastname: Yang
authors.affiliation: Department of Endocrinology, Tangshan Workers' Hospital, Tangshan, Hebei 063000, P.R. China.
authors.forename: Jinli
authors.initials: J
authors.lastname: Tian
authors.affiliation: Department of Endocrinology, Tangshan Workers' Hospital, Tangshan, Hebei 063000, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of Internal Medicine, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Medicine, Hebei United University, Tangshan, Hebei 063000, P.R. China.
authors.forename: Ruhua
authors.initials: R
authors.lastname: Guan
authors.affiliation: Department of Internal Medicine, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China.
authors.forename: Mingyue
authors.initials: M
authors.lastname: Qiu
authors.affiliation: Department of Internal Medicine, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China.
authors.forename: Yukai
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Endocrinology, Tangshan Workers' Hospital, Tangshan, Hebei 063000, P.R. China.
authors.forename: Xueling
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Endocrinology, Tangshan Workers' Hospital, Tangshan, Hebei 063000, P.R. China.
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Zhen
authors.affiliation: Department of Endocrinology, Tangshan Workers' Hospital, Tangshan, Hebei 063000, P.R. China.
authors.forename: Yazhong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Endocrinology, Tangshan Workers' Hospital, Tangshan, Hebei 063000, P.R. China.
authors.forename: Chunyou
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Endocrinology, Tangshan Workers' Hospital, Tangshan, Hebei 063000, P.R. China.
authors.forename: Yanbing
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Internal Medicine, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China; Department of Endocrinology, Tangshan Workers' Hospital, Tangshan, Hebei 063000, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Fang
keywords: cancer; long noncoding RNA; mRNA; microarray; papillary thyroid carcinoma