title: Genomic integration occurs in the packaging cell via unexported lentiviral precursors.
abstract: OBJECTIVE
  To use HIV-1 based lentivirus components to produce gene integration and the formation of a stable cell line in the packaging cell line without viral infection.
  
  
  RESULTS
  A co-transfection of a Human Embryonic Kidney (HEK) 293 packaging cell line with Gag-pol (GP) and a transfer vector, without the envelope vector, produces a stable cell line after 2 weeks of selection. Furthermore, a matrix protein deficient GP in the packaging vector enhances this integration. This supports that, in theory, unexported lentiviral cores produced within the packaging cell can infect itself without requiring the release of any lentiviral particles.
  
  
  CONCLUSIONS
  If the packaging cell is also the target cell, then gene integration leading to a stable cell line can be accomplished without viral particle infection.
authors.affiliation: Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Toronto, ON, M5S 3G9, Canada.
authors.forename: Abdullah Al
authors.initials: AA
authors.lastname: Mosabbir
authors.affiliation: Institute of Biomaterials and Biomedical Engineering, University of Toronto, 164 College Street, Toronto, ON, M5S 3G9, Canada. kevin.truong@utoronto.ca.|Edward S. Rogers Sr. Department of Electrical and Computer Engineering, University of Toronto, 10 King's College Circle, Toronto, ON, M5S 3G4, Canada. kevin.truong@utoronto.ca.
authors.forename: Kevin
authors.initials: K
authors.lastname: Truong
mesh_terms: D016475:3T3 Cells; D000818:Animals; D042003:DNA Packaging; D015685:Fusion Proteins, gag-pol / Q000235:genetics*; D005822:Genetic Vectors; D057809:HEK293 Cells; D015497:HIV-1 / Q000235:genetics*; D006801:Humans; D051379:Mice; D014162:Transfection / Q000379:methods*
chemical_list: D015685:Fusion Proteins, gag-pol
keywords: Gene integration; Human embryonic kidney cell; Lentivirus; Stable cell line