title: Galectin-1-induced skeletal muscle cell differentiation of mesenchymal stem cells seeded on an acellular dermal matrix improves injured anal sphincter.
abstract: According to recent studies, mesenchymal stromal cells (MSCs) transplanted via local or tail vein injection can improve healing after anal sphincter injury (ASI) in animal models. However, the transplanted MSCs do not generate skeletal muscle that completely resembles the natural anal sphincter structure. In the present study, we investigated whether bone marrow (BM)-derived MSCs could be induced by Galectin-1 (Gal-1) to differentiate into skeletal muscle and whether the recellularization of an acellular dermal matrix (ADM) with skeletal muscle-differentiated MSCs represents a promising approach to restore ASI in a rat model. BM-MSCs subjected to adenovirus-mediated transfection with Gal-1-GFP (Ad-GFP-Gal-1) displayed increased Gal-1 and desmin expression and differentiated into skeletal muscle cells. MSCs transfected with Ad-GFP-Gal-1 (MSC-Gal-1) were seeded onto an ADM (ADM-MSC-Gal-1) via co-culture, and fusion was observed using a confocal laser scanning microscope. ADM-MSC-Gal-1, ADM-MSC, ADM-MSC-Ad, ADM, or a saline control was applied to a rat ASI model, and injury healing was evaluated via histological examination 6 weeks following treatment. ADM-MSC-Gal-1 treatment promoted significant healing after ASI and improved external anal sphincter contraction curves compared with the other treatments and also led to substantial skeletal muscle regeneration and neovascularization. Our results indicate that repair using ADMs and differentiated MSCs may improve muscle regeneration and restore ASI.
authors.affiliation: Department of Colorectal Surgery, Zhongnan Hospital, Wuhan University; Hubei Key Laboratory of Intestinal and Colorectal Diseases, Clinical Research Center for Intestinal and Colorectal Diseases of Hubei Province; Wuhan, Hubei 430071, China.|These authors contributed equally to this work.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Ding
authors.affiliation: Department of General Surgery, Wuhan No. 1 Hospital, Wuhan, Hubei 430022, China.|These authors contributed equally to this work.
authors.forename: Xiangui
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Colorectal Surgery, Zhongnan Hospital, Wuhan University; Hubei Key Laboratory of Intestinal and Colorectal Diseases, Clinical Research Center for Intestinal and Colorectal Diseases of Hubei Province; Wuhan, Hubei 430071, China.
authors.forename: Xianghai
authors.initials: X
authors.lastname: Ren
authors.affiliation: Department of Colorectal Surgery, Zhongnan Hospital, Wuhan University; Hubei Key Laboratory of Intestinal and Colorectal Diseases, Clinical Research Center for Intestinal and Colorectal Diseases of Hubei Province; Wuhan, Hubei 430071, China.
authors.forename: Qiulei
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Colorectal Surgery, Zhongnan Hospital, Wuhan University; Hubei Key Laboratory of Intestinal and Colorectal Diseases, Clinical Research Center for Intestinal and Colorectal Diseases of Hubei Province; Wuhan, Hubei 430071, China.
authors.forename: Tingtao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Department of Colorectal Surgery, Zhongnan Hospital, Wuhan University; Hubei Key Laboratory of Intestinal and Colorectal Diseases, Clinical Research Center for Intestinal and Colorectal Diseases of Hubei Province; Wuhan, Hubei 430071, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Qian
authors.affiliation: Department of Colorectal Surgery, Zhongnan Hospital, Wuhan University; Hubei Key Laboratory of Intestinal and Colorectal Diseases, Clinical Research Center for Intestinal and Colorectal Diseases of Hubei Province; Wuhan, Hubei 430071, China.
authors.forename: Weicheng
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Colorectal Surgery, Zhongnan Hospital, Wuhan University; Hubei Key Laboratory of Intestinal and Colorectal Diseases, Clinical Research Center for Intestinal and Colorectal Diseases of Hubei Province; Wuhan, Hubei 430071, China.
authors.forename: Congqing
authors.initials: C
authors.lastname: Jiang
mesh_terms: D062088:Acellular Dermis; D000256:Adenoviridae / Q000235:genetics; D001003:Anal Canal / Q000293:injuries*; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D004195:Disease Models, Animal; D005240:Feasibility Studies; D005434:Flow Cytometry; D037483:Galectin 1 / Q000235:genetics / Q000502:physiology*; D005822:Genetic Vectors / Q000008:administration & dosage; D006801:Humans; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D018482:Muscle, Skeletal / Q000502:physiology*; D018919:Neovascularization, Physiologic; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration / Q000502:physiology*; D014162:Transfection; D014945:Wound Healing / Q000502:physiology*
chemical_list: D037483:Galectin 1