title: Rubisco Catalytic Properties and Temperature Response in Crops.
abstract: Rubisco catalytic traits and their thermal dependence are two major factors limiting the CO2 assimilation potential of plants. In this study, we present the profile of Rubisco kinetics for 20 crop species at three different temperatures. The results largely confirmed the existence of significant variation in the Rubisco kinetics among species. Although some of the species tended to present Rubisco with higher thermal sensitivity (e.g. Oryza sativa) than others (e.g. Lactuca sativa), interspecific differences depended on the kinetic parameter. Comparing the temperature response of the different kinetic parameters, the Rubisco Km for CO2 presented higher energy of activation than the maximum carboxylation rate and the CO2 compensation point in the absence of mitochondrial respiration. The analysis of the Rubisco large subunit sequence revealed the existence of some sites under adaptive evolution in branches with specific kinetic traits. Because Rubisco kinetics and their temperature dependency were species specific, they largely affected the assimilation potential of Rubisco from the different crops, especially under those conditions (i.e. low CO2 availability at the site of carboxylation and high temperature) inducing Rubisco-limited photosynthesis. As an example, at 25°C, Rubisco from Hordeum vulgare and Glycine max presented, respectively, the highest and lowest potential for CO2 assimilation at both high and low chloroplastic CO2 concentrations. In our opinion, this information is relevant to improve photosynthesis models and should be considered in future attempts to design more efficient Rubiscos.
authors.affiliation: Research Group on Plant Biology under Mediterranean Conditions, Universitat de les Illes Balears, 07122 Palma, Balearic Islands, Spain (C.H.-C., J.G.); andSchool of Natural Sciences and Psychology, Liverpool John Moores University, Liverpool L3 3AF, United Kingdom (M.V.K.).
authors.forename: Carmen
authors.identifier: 0000-0003-3272-3054
authors.initials: C
authors.lastname: Hermida-Carrera
authors.affiliation: Research Group on Plant Biology under Mediterranean Conditions, Universitat de les Illes Balears, 07122 Palma, Balearic Islands, Spain (C.H.-C., J.G.); andSchool of Natural Sciences and Psychology, Liverpool John Moores University, Liverpool L3 3AF, United Kingdom (M.V.K.).
authors.forename: Maxim V
authors.identifier: 0000-0001-7966-0295
authors.initials: MV
authors.lastname: Kapralov
authors.affiliation: Research Group on Plant Biology under Mediterranean Conditions, Universitat de les Illes Balears, 07122 Palma, Balearic Islands, Spain (C.H.-C., J.G.); andSchool of Natural Sciences and Psychology, Liverpool John Moores University, Liverpool L3 3AF, United Kingdom (M.V.K.) jeroni.galmes@uib.cat.
authors.forename: Jeroni
authors.identifier: 0000-0002-7299-9349
authors.initials: J
authors.lastname: Galmés
mesh_terms: D055162:Biocatalysis; D002245:Carbon Dioxide / Q000378:metabolism; D018556:Crops, Agricultural / Q000201:enzymology* / Q000502:physiology*; D007700:Kinetics; D010802:Phylogeny; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D012273:Ribulose-Bisphosphate Carboxylase / Q000378:metabolism*; D013696:Temperature
chemical_list: D021122:Protein Subunits; D002245:Carbon Dioxide; D012273:Ribulose-Bisphosphate Carboxylase