title: Antihypoxic effect of miR-24 in SH-SY5Y cells under hypoxia via downregulating expression of neurocan.
abstract: Hypoxia-induced apoptosis-related mechanisms involved in the brain damage following cerebral ischemia injury. A subset of the small noncoding microRNA (miRNAs) is regulated by tissue oxygen levels, and miR-24 was found to be activated by hypoxic conditions. However, the roles of miR-24 and its target gene in neuron are not well understood. Here, we validated miRNA-24 is down-regulated in patients with cerebral infarction. Hypoxia suppressed the expression of miR-24, but increased the expression of neurocan in both mRNA and protein levels in SH-SY5Y cells. MiR-24 mimics reduced the expression of neurocan, suppressed cell apoptosis, induced cell cycle progression and cell proliferation in SH-SY5Y cells under hypoxia. By luciferase reporter assay, neurocan is validated a direct target gene of miR-24. Furthermore, knockdown of neurocan suppressed cell apoptosis, induced cell cycle progression and cell proliferation in SH-SY5Y cells under hypoxia. Taken together, miR-24 overexpression or silencing of neurocan shows an antihypoxic effect in SH-SY5Y cells. Therefore, miR-24 and neurocan play critical roles in neuron cell apoptosis and are potential therapeutic targets for ischemic brain disease.
authors.affiliation: Department of Neurology, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar, 161000, People's Republic of China. Electronic address: sunxingyuan@sina.com.
authors.forename: Xingyuan
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Neurology, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar, 161000, People's Republic of China.
authors.forename: Zhanjun
authors.initials: Z
authors.lastname: Ren
authors.affiliation: Department of Neurology, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar, 161000, People's Republic of China.
authors.forename: Yunzhi
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Department of Neurology, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar, 161000, People's Republic of China.
authors.forename: Chenxin
authors.initials: C
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D015687:Cell Hypoxia / Q000502:physiology*; D002460:Cell Line; D015536:Down-Regulation / Q000502:physiology; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D009474:Neurons / Q000166:cytology* / Q000502:physiology*
chemical_list: C543845:MIRN24 microRNA, human; D035683:MicroRNAs
keywords: Cerebral infarction; Hypoxia; Neurocan; miR-24