title: MicroRNA‑335 inhibits bladder cancer cell growth and migration by targeting mitogen‑activated protein kinase 1.
abstract: The abnormal expression of microRNAs (miRs) as oncogenes or tumor‑suppressor genes has been widely investigated in various tumor types. However, the roles of miR‑335 in bladder cancer cells have remained elusive. The aim of the present study was to assess the expression of miR‑335 in bladder cancer as well as the effects of miR‑335 on bladder cancer cell proliferation, metastasis and apoptosis. PCR and western blot analyses revealed that miR‑335 was significantly downregulated in bladder cancer tissues, and low levels of miR‑335 were associated with more aggressive phenotypes of bladder cancer. Overexpression of miR‑335 in T24 cells inhibited cell proliferation and induced apoptosis as indicated by an MTT assay and flow cytometric analysis, respectively. Furthermore, overexpression of miR‑335 significantly suppressed cell migration, as indicated by a Transwell assay. The expression of mitogen‑activated protein kinase (MAPK)1 was decreased after overexpression of miR‑335, indicating that MAPK1 may be a target gene of miR‑335. In addition, silencing of MAPK1 inhibited the proliferation and migration of bladder cancer cells. In conclusion, the results of the present study demonstrated that miR‑335 was significantly downregulated in bladder cancer, and may act as a tumor suppressor through repression of MAPK1.
authors.affiliation: Department of Urology, Nantong Tumor Hospital, Nantong, Jiangsu 226361, P.R. China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Urology, The First Hospital of Wujiang, Suzhou, Jiangsu 215200, P.R. China.
authors.forename: Guang
authors.initials: G
authors.lastname: Wu
authors.affiliation: Department of Urology, Nantong Tumor Hospital, Nantong, Jiangsu 226361, P.R. China.
authors.forename: Guangxin
authors.initials: G
authors.lastname: Cao
authors.affiliation: Department of Urology, Nantong Tumor Hospital, Nantong, Jiangsu 226361, P.R. China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Urology, Nantong Tumor Hospital, Nantong, Jiangsu 226361, P.R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Urology, Nantong Tumor Hospital, Nantong, Jiangsu 226361, P.R. China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Department of Urology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China.
authors.forename: Jianquan
authors.initials: J
authors.lastname: Hou
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D019950:Mitogen-Activated Protein Kinase 1 / Q000235:genetics*; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D034622:RNA Interference; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: C556347:MIRN335 microRNA, human; D035683:MicroRNAs; D019950:Mitogen-Activated Protein Kinase 1