title: Analysis of Spleen Cells in Susceptible and Resistant Mice with Experimental Lagochilascariosis.
abstract: Lagochilascariosis is an emerging parasitic disease caused by the helminth Lagochilascaris minor. The experimental mouse model has been used to study the immune response against L. minor infection. In the present work, immunohistochemistry analysis of spleen cells populations was evaluated in susceptible (C57BL/6) and resistant (BALB/c) mice experimentally infected with L. minor. The BALB/c mice exhibited increased spleen cell indexes as follows: F4/80+ at 100 days after infection (DPI), CD4+ at 100 and 250 DPI, CD8+ at 35 and 100 DPI, and CD19+ at 100, 150, and 250 DPI. In the spleens of the infected C57BL/6 mice, increased indexes of the following spleen cells were observed: F4/80+ cells at 250 DPI, CD4+ cells at 150 DPI, CD8+ cells at 35, 150, and 250 DPI, and CD19+ cells at 150 to 250 DPI. The index of spleen cells confirmed the differences between the control and infected groups at several time points following the infection. These data demonstrate an association between a preferential increase in the number of CD4+ and CD19+ spleen cells and resistance to experimental lagochilascariosis in BALB/c mice and between a preferential increase in the number of CD8+ spleen cells and susceptibility in C57BL/6 mice.
authors.affiliation: Laboratory of Immunochemistry, Butantan Institute, São Paulo, SP, Brazil; Laboratory of Immunogenetics, Butantan Institute, São Paulo, SP, Brazil.
authors.forename: Priscila Guirão
authors.initials: PG
authors.lastname: Lara
authors.affiliation: Department of Microbiology, Immunology, Parasitology and Pathology, Institute of Tropical Pathology and Public Health, Federal University of Goiás, Goiânia, GO, Brazil.
authors.forename: Mariana Felix de Souza
authors.initials: MF
authors.lastname: Prudente
authors.affiliation: Department of Microbiology, Immunology, Parasitology and Pathology, Institute of Tropical Pathology and Public Health, Federal University of Goiás, Goiânia, GO, Brazil.
authors.forename: Neusa Mariana Costa
authors.initials: NM
authors.lastname: Dias
authors.affiliation: Laboratory of Immunochemistry, Butantan Institute, São Paulo, SP, Brazil.
authors.forename: Denise Vilarinho
authors.initials: DV
authors.lastname: Tambourgi
authors.affiliation: Department of Microbiology, Immunology, Parasitology and Pathology, Institute of Tropical Pathology and Public Health, Federal University of Goiás, Goiânia, GO, Brazil.
authors.forename: Ruy de Souza
authors.initials: Rde S
authors.lastname: Lino-Junior
authors.affiliation: Laboratory of Immunochemistry, Butantan Institute, São Paulo, SP, Brazil; Laboratory of Immunogenetics, Butantan Institute, São Paulo, SP, Brazil.
authors.forename: Mônica
authors.initials: M
authors.lastname: Spadafora-Ferreira
authors.affiliation: Department of Microbiology, Immunology, Parasitology and Pathology, Institute of Tropical Pathology and Public Health, Federal University of Goiás, Goiânia, GO, Brazil.
authors.forename: Mara Silvia
authors.initials: MS
authors.lastname: Carvalhaes