title: Hydrogen Peroxide-Resistant CotA and YjqC of Bacillus altitudinis Spores Are a Promising Biocatalyst for Catalyzing Reduction of Sinapic Acid and Sinapine in Rapeseed Meal.
abstract: For the more efficient detoxification of phenolic compounds, a promising avenue would be to develop a multi-enzyme biocatalyst comprising peroxidase, laccase and other oxidases. However, the development of this multi-enzyme biocatalyst is limited by the vulnerability of fungal laccases and peroxidases to hydrogen peroxide (H2O2)-induced inactivation. Therefore, H2O2-resistant peroxidase and laccase should be exploited. In this study, H2O2-stable CotA and YjqC were isolated from the outer coat of Bacillus altitudinis SYBC hb4 spores. In addition to the thermal and alkali stability of catalytic activity, CotA also exhibited a much higher H2O2 tolerance than fungal laccases from Trametes versicolor and Trametes trogii. YjqC is a sporulation-related manganese (Mn) catalase with striking peroxidase activity for sinapic acid (SA) and sinapine (SNP). In contrast to the typical heme-containing peroxidases, the peroxidase activity of YjqC was also highly resistant to inhibition by H2O2 and heat. CotA could also catalyze the oxidation of SA and SNP. CotA had a much higher affinity for SA than B. subtilis CotA. CotA and YjqC rendered from B. altitudinis spores had promising laccase and peroxidase activities for SA and SNP. Specifically, the B. altitudinis spores could be regarded as a multi-enzyme biocatalyst composed of CotA and YjqC. The B. altitudinis spores were efficient for catalyzing the degradation of SA and SNP in rapeseed meal. Moreover, efficiency of the spore-catalyzed degradation of SA and SNP was greatly improved by the presence of 15 mM H2O2. This effect was largely attributed to synergistic biocatalysis of the H2O2-resistant CotA and YjqC toward SA and SNP.
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, China.
authors.forename: Yanzhou
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, China.|The Bioscience and Engineering College, Jiangxi Agriculture University, Nanchang, 330045, China.
authors.forename: Xunhang
authors.initials: X
authors.lastname: Li
authors.affiliation: Institute of Applied Biotechnology, Taizhou Vocational & Technical College, Taizhou, 318000, China.
authors.forename: Zhikui
authors.initials: Z
authors.lastname: Hao
authors.affiliation: College of Forestry, South China Agricultural University, Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, Guangzhou, 510642, China.
authors.forename: Ruchun
authors.initials: R
authors.lastname: Xi
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Cai
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, China.
authors.forename: Xiangru
authors.initials: X
authors.lastname: Liao
mesh_terms: D001407:Bacillus / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000502:physiology; D055162:Biocatalysis; D019149:Bioreactors / Q000382:microbiology; D029741:Brassica rapa / Q000378:metabolism*; D002374:Catalase / Q000235:genetics / Q000502:physiology*; D002384:Catalysis; D002794:Choline / Q000031:analogs & derivatives* / Q000378:metabolism; D003373:Coumaric Acids / Q000378:metabolism*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D006861:Hydrogen Peroxide / Q000494:pharmacology; D042845:Laccase / Q000235:genetics / Q000502:physiology*; D010084:Oxidation-Reduction; D013171:Spores, Bacterial / Q000737:chemistry / Q000378:metabolism
chemical_list: D001425:Bacterial Outer Membrane Proteins; D003373:Coumaric Acids; C005685:sinapine; C073734:sinapinic acid; D006861:Hydrogen Peroxide; D042845:Laccase; D002374:Catalase; D002794:Choline