title: Derivation of HVR1, HVR2 and HVR3 human embryonic stem cell lines from IVF embryos after preimplantation genetic diagnosis (PGD) for monogenic disorder.
abstract: From 106 human blastocyts donate for research after in vitro fertilization (IVF) and preimplantation genetic diagnosis (PGD) for monogenetic disorder, 3 human embryonic stem cells (hESCs) HVR1, HVR2 and HVR3 were successfully derived. HVR1 was assumed to be genetically normal, HVR2 carrying Becker muscular dystrophy and HVR3 Hemophilia B. Despite the translocation t(9;15)(q34.3;q14) detected in HVR2, all the 3 cell lines were characterised in vitro and in vivo as normal hESCs lines and were registered in the Spanish Stem Cell Bank.
authors.affiliation: Department of Stem Cells, Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER), Seville 41092, Spain; Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólica asociada (CIBERDEM), Madrid 28029, Spain. Electronic address: karim.hmadcha@cabimer.es.
authors.forename: Abdelkrim
authors.initials: A
authors.lastname: Hmadcha
authors.affiliation: Department of Stem Cells, Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER), Seville 41092, Spain.
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Aguilera
authors.affiliation: Department of Genetics, Reproduction and Fetal Medicine, Instituto de Biomedicina de Sevilla (IBiS), Seville 41013, Spain.
authors.forename: Maria Dolores
authors.initials: MD
authors.lastname: Lozano-Arana
authors.affiliation: Department of Stem Cells, Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER), Seville 41092, Spain.
authors.forename: Nuria
authors.initials: N
authors.lastname: Mellado
authors.affiliation: Department of Genetics, Reproduction and Fetal Medicine, Instituto de Biomedicina de Sevilla (IBiS), Seville 41013, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Sánchez
authors.affiliation: Department of Genetics, Reproduction and Fetal Medicine, Instituto de Biomedicina de Sevilla (IBiS), Seville 41013, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Moya
authors.affiliation: Department of Stem Cells, Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER), Seville 41092, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Sánchez-Palazón
authors.affiliation: Department of Pathology, Instituto de Biomedicina de Sevilla (IBiS), Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER), Seville 41013, Spain; Department of Pathology, Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid 28034, Spain.
authors.forename: Jose
authors.initials: J
authors.lastname: Palacios
authors.affiliation: Department of Genetics, Reproduction and Fetal Medicine, Instituto de Biomedicina de Sevilla (IBiS), Seville 41013, Spain; Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Madrid 28029, Spain.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Antiñolo
authors.affiliation: Department of Stem Cells, Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER), Seville 41092, Spain; Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólica asociada (CIBERDEM), Madrid 28029, Spain.
authors.forename: Bernat
authors.initials: B
authors.lastname: Soria
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology; D002454:Cell Differentiation; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D002884:Chromosomes, Human, Pair 15; D002899:Chromosomes, Human, Pair 9; D005260:Female; D005307:Fertilization in Vitro; D002836:Hemophilia B / Q000175:diagnosis* / Q000235:genetics; D000066449:Human Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation; D006801:Humans; D059785:Karyotype; D008297:Male; D051379:Mice; D016513:Mice, SCID; D008856:Microscopy, Fluorescence; D009136:Muscular Dystrophies / Q000175:diagnosis* / Q000235:genetics; D019836:Preimplantation Diagnosis; D013724:Teratoma / Q000378:metabolism / Q000473:pathology; D014178:Translocation, Genetic