title: Alphacoronavirus in urban Molossidae and Phyllostomidae bats, Brazil.
abstract: Bats have been implicated as the main reservoir of coronavirus (CoV). Thus the role of these hosts on the evolution and spread of CoVs currently deserve the attention of emerging diseases surveillance programs. On the view of the interest on and importance of CoVs in bats the occurrence and molecular characterization of CoV were conducted in bats from Brazil.
  
  
  
  Three hundred five enteric contents of 29 bat species were tested using a panCoV nested RT-PCR. Nine specimens were positive and eight was suitable for RdRp gene sequencing. RdRp gene phylogeny showed that all CoVs strains from this study cluster in Alphacoronavirus genus, with one Molossidae and one Phlyllostomidae-CoV specific groups. Phylogenetic analyses of two S gene sequences showed a large diversity within the Alphacoronavirus genus.
  
  
  
  This study indicated a CoV-to-host specificity and draws attention for CoV detection in Cynomops sp, a potential new reservoir. The phylogenetic analyses indicate that diversity of CoV in bats is higher than previously known.
authors.affiliation: Instituto Pasteur, Av. Paulista, 393, CEP:01311-000, São Paulo, SP, Brazil. karen.asano@gmail.com.|Departament of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine, University of São Paulo, Av. Orlando Marques de Paiva, 87, CEP: 05508-270, São Paulo, Brazil. karen.asano@gmail.com.
authors.forename: Karen Miyuki
authors.initials: KM
authors.lastname: Asano
authors.affiliation: Departament of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine, University of São Paulo, Av. Orlando Marques de Paiva, 87, CEP: 05508-270, São Paulo, Brazil.
authors.forename: Aline Santana
authors.initials: AS
authors.lastname: Hora
authors.affiliation: Instituto Pasteur, Av. Paulista, 393, CEP:01311-000, São Paulo, SP, Brazil.
authors.forename: Karin Côrrea
authors.initials: KC
authors.lastname: Scheffer
authors.affiliation: Instituto Pasteur, Av. Paulista, 393, CEP:01311-000, São Paulo, SP, Brazil.
authors.forename: Willian Oliveira
authors.initials: WO
authors.lastname: Fahl
authors.affiliation: Instituto Pasteur, Av. Paulista, 393, CEP:01311-000, São Paulo, SP, Brazil.
authors.forename: Keila
authors.initials: K
authors.lastname: Iamamoto
authors.affiliation: Instituto Pasteur, Av. Paulista, 393, CEP:01311-000, São Paulo, SP, Brazil.
authors.forename: Enio
authors.initials: E
authors.lastname: Mori
authors.affiliation: Departament of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine, University of São Paulo, Av. Orlando Marques de Paiva, 87, CEP: 05508-270, São Paulo, Brazil.
authors.forename: Paulo Eduardo
authors.initials: PE
authors.lastname: Brandão
mesh_terms: D000818:Animals; D001938:Brazil; D002685:Chiroptera / Q000821:virology*; D017934:Coronavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D018352:Coronavirus Infections / Q000662:veterinary* / Q000821:virology; D016679:Genome, Viral; D010802:Phylogeny
keywords: Bat; Coronavirus; Molossidae; Phyllostomidae