title: Construction of local gene network for revealing different liver function of rats fed deep-fried oil with or without resistant starch.
abstract: To study the mechanism underlying the liver damage induced by deep-fried oil (DO) consumption and the beneficial effects from resistant starch (RS) supplement, differential gene expression and pathway network were analyzed based on RNA sequencing data from rats. The up/down regulated genes and corresponding signaling pathways were used to construct a novel local gene network (LGN). The topology of the network showed characteristics of small-world network, with some pathways demonstrating a high degree. Some changes in genes led to a larger probability occurrence of disease or infection with DO intake. More importantly, the main pathways were found to be almost the same between the two LGNs (30 pathways overlapped in total 48) with gene expression profile. This finding may indicate that RS supplement in DO-containing diet may mainly regulate the genes that related to DO damage, and RS in the diet may provide direct signals to the liver cells and modulate its effect through a network involving complex gene regulatory events. It is the first attempt to reveal the mechanism of the attenuation of liver dysfunction from RS supplement in the DO-containing diet using differential gene expression and pathway network.
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, China; Tianjin Food Safety & Low Carbon Manufacturing Collaborative Innovation Center, 300457 Tianjin, China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, China.
authors.forename: Tianqi
authors.initials: T
authors.lastname: Liao
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, China; Tianjin Food Safety & Low Carbon Manufacturing Collaborative Innovation Center, 300457 Tianjin, China; ARC Industrial Transformation Training Centre for Functional Grains, Charles Sturt University, Wagga Wagga, NSW 2678, Australia. Electronic address: zkzhou@tust.edu.cn.
authors.forename: Zhongkai
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, China.
authors.forename: Yuyang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, China.
authors.forename: Yongjia
authors.initials: Y
authors.lastname: Diao
authors.affiliation: ARC Industrial Transformation Training Centre for Functional Grains, Charles Sturt University, Wagga Wagga, NSW 2678, Australia.
authors.forename: Padraig
authors.initials: P
authors.lastname: Strappe
authors.affiliation: ARC Industrial Transformation Training Centre for Functional Grains, Charles Sturt University, Wagga Wagga, NSW 2678, Australia.
authors.forename: Paul
authors.initials: P
authors.lastname: Prenzler
authors.affiliation: NSW Department of Primary Industries, Agriculture Institute, Wagga Wagga, NSW 2650, Australia.
authors.forename: Jamie
authors.initials: J
authors.lastname: Ayton
authors.affiliation: ARC Industrial Transformation Training Centre for Functional Grains, Charles Sturt University, Wagga Wagga, NSW 2678, Australia.
authors.forename: Chris
authors.initials: C
authors.lastname: Blanchard
mesh_terms: D000818:Animals; D059305:Diet, High-Fat / Q000009:adverse effects*; D004042:Dietary Fats, Unsaturated / Q000009:adverse effects / Q000032:analysis; D019587:Dietary Supplements; D004063:Digestion; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D015723:Gene Library; D053263:Gene Regulatory Networks; D048550:Hepatic Insufficiency / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology / Q000517:prevention & control*; D006358:Hot Temperature / Q000009:adverse effects; D008099:Liver / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D054647:Nutrigenomics / Q000379:methods; D010938:Plant Oils / Q000009:adverse effects / Q000737:chemistry; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism; D011897:Random Allocation; D000074262:Rapeseed Oil; D017208:Rats, Wistar; D017423:Sequence Analysis, RNA; D015398:Signal Transduction; D013213:Starch / Q000378:metabolism / Q000627:therapeutic use*
chemical_list: D004042:Dietary Fats, Unsaturated; D010938:Plant Oils; D012333:RNA, Messenger; D000074262:Rapeseed Oil; D013213:Starch
keywords: Deep-fried oil; Gene network; Resistant starch; Signaling pathway