title: Accelerating effects of cellulase in the removal of denture adhesives from acrylic denture bases.
abstract: OBJECTIVE
  Studies of effective methods for the easy removal of denture adhesives from a denture base are not well represented in the literature. We previously assessed the removability of denture adhesives by immersing within denture cleaners, showing that some cleaners have a weak effect, insufficiently effective in daily use. In this study, we prepared a cellulase, as a potential component for denture adhesive removers, and we examined whether the addition of cellulase to denture cleaners is effective in the removal of cream denture adhesives.
  
  
  METHODS
  We prepared the cellulase Meicelase as one component for the liquefaction of denture adhesives. We used two denture cleaners and two cream adhesives. After the immersion of plates in sample solutions, we evaluated the area of the sample plate still covered with adhesives. Biofilm removal assay was also performed using denture cleaners containing cellulase.
  
  
  RESULTS
  The addition of cellulase accelerated the removal of cream adhesives in immersion experiments to a rate faster than that of water and denture cleaners. However, it did not influence the removability of Candida albicans biofilms from acrylic resin specimens.
  
  
  CONCLUSIONS
  Cellulase hastened the liquefaction of cream adhesives.
authors.affiliation: Department of Oral and Maxillofacial Prosthodontics, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan; Department of Cellular and Molecular Pharmacology, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan. Electronic address: hkae@dent.kagoshima-u.ac.jp.
authors.forename: Kae
authors.initials: K
authors.lastname: Harada-Hada
authors.affiliation: Department of Oral Biology and Engineering, Institute of Biomedical and Health Science, Hiroshima University, Hiroshima, Japan.
authors.forename: Sumiyo
authors.initials: S
authors.lastname: Mimura
authors.affiliation: Liaison Center for Innovative Dentistry, Graduate School of Dentistry, Tohoku University, Sendai, Japan.
authors.forename: Guang
authors.initials: G
authors.lastname: Hong
authors.affiliation: Department of Oral Biology and Engineering, Institute of Biomedical and Health Science, Hiroshima University, Hiroshima, Japan.
authors.forename: Tatsumi
authors.initials: T
authors.lastname: Hashida
authors.affiliation: Department of Advanced Prosthodontics, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Abekura
authors.affiliation: Department of Prosthetic Dentistry, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Murata
authors.affiliation: Department of Oral and Maxillofacial Prosthodontics, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Nishimura
authors.affiliation: Department of Oral Biology and Engineering, Institute of Biomedical and Health Science, Hiroshima University, Hiroshima, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Nikawa
mesh_terms: D000180:Acrylic Resins; D018441:Biofilms; D002176:Candida albicans / Q000502:physiology; D002480:Cellulase; D003738:Dental Cements; D003819:Denture Bases / Q000382:microbiology; D003820:Denture Cleansers / Q000737:chemistry; D012117:Resins, Synthetic
chemical_list: D000180:Acrylic Resins; D003738:Dental Cements; D003820:Denture Cleansers; D012117:Resins, Synthetic; D002480:Cellulase
keywords: Candida albicans biofilm; Cellulase; Denture adhesive; Denture cleaner; Meicelase