title: Altered striatal rhythmic activity in cylindromatosis knock-out mice due to enhanced GABAergic inhibition.
abstract: Despite the highest expression in striatum, physiological function of cylindromatosis (CYLD), a deubiquitinating enzyme, remains unexplored. We found, in the present study, that the duration of spontaneous up-states in the striatum is shorter and membrane potential fluctuation preceding action potential and firing rate are increased in Cyld(-/-) mice. Excess striatal GABAergic inhibition likely plays the major role in this alteration as supported by the findings: (1) the levels of striatal GABAA and GABAB receptors in Cyld(-/-) mice are increased, (2) pharmacological blockade of GABAB receptors rescues the shortened up-state phenotype, and (3) pharmacological blockade of GABAA receptors rescues the power of beta frequency oscillations. Our results indicate that CYLD alters striatal network function by regulating the protein expression levels of GABA receptors.
authors.affiliation: School of Life Sciences, South China Normal University, Guangzhou 510631, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: School of Life Sciences, South China Normal University, Guangzhou 510631, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Chen
authors.affiliation: School of Life Sciences, South China Normal University, Guangzhou 510631, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.affiliation: School of Life Sciences, South China Normal University, Guangzhou 510631, PR China.
authors.forename: Binghua
authors.initials: B
authors.lastname: Lv
authors.affiliation: School of Life Sciences, South China Normal University, Guangzhou 510631, PR China.
authors.forename: Kai
authors.initials: K
authors.lastname: Jin
authors.affiliation: School of Life Sciences, South China Normal University, Guangzhou 510631, PR China.
authors.forename: Shudan
authors.initials: S
authors.lastname: Zheng
authors.affiliation: School of Psychology, South China Normal University, Guangzhou 510631, PR China; Brain Science Institute, South China Normal University, Guangzhou 510631, PR China; Guangdong Key Laboratory of Mental Health and Cognitive Science, South China Normal University, Guangzhou 510631, PR China; Center for Studies of Psychological Application, South China Normal University, Guangzhou 510631, PR China. Electronic address: yang_li@m.scnu.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.affiliation: School of Life Sciences, South China Normal University, Guangzhou 510631, PR China; Brain Science Institute, South China Normal University, Guangzhou 510631, PR China. Electronic address: longcheng@m.scnu.edu.cn.
authors.forename: Cheng
authors.initials: C
authors.lastname: Long
mesh_terms: D000818:Animals; D001611:Beta Rhythm / Q000187:drug effects / Q000502:physiology*; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D003546:Cysteine Endopeptidases / Q000172:deficiency* / Q000235:genetics; D000074749:Deubiquitinating Enzyme CYLD; D058787:GABA-A Receptor Antagonists / Q000494:pharmacology; D058788:GABA-B Receptor Antagonists / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009433:Neural Inhibition / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D011963:Receptors, GABA-A / Q000378:metabolism*; D018080:Receptors, GABA-B / Q000378:metabolism*; D012259:Ribonuclease, Pancreatic / Q000378:metabolism; D046509:Tissue Culture Techniques; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D058787:GABA-A Receptor Antagonists; D058788:GABA-B Receptor Antagonists; D011963:Receptors, GABA-A; D018080:Receptors, GABA-B; D005680:gamma-Aminobutyric Acid; C099439:Ang2 protein, mouse; D012259:Ribonuclease, Pancreatic; C511601:CYLD protein, mouse; D000074749:Deubiquitinating Enzyme CYLD; D003546:Cysteine Endopeptidases
keywords: Cyld; Down-states; GABA receptor; Striatum; Up-states