title: Antibody neutralization of cell-surface gC1qR/HABP1/SF2-p32 prevents lamellipodia formation and tumorigenesis.
abstract: We previously demonstrated that cell-surface gC1qR is a key regulator of lamellipodia formation and cancer metastasis. Here, we screened a monoclonal mouse antibody against gC1qR to prevent cell migration by neutralizing cell-surface gC1qR. The anti-gC1qR antibody prevented growth factor-stimulated lamellipodia formation, cell migration and focal adhesion kinase activation by inactivating receptor tyrosine kinases (RTKs) in various cancer cells such as A549, MDA-MB-231, MCF7 and HeLa cells. The antibody neutralization of cell-surface gC1qR also inhibited angiogenesis because the anti-gC1qR antibody prevented growth factor-stimulated RTK activation, lamellipodia formation, cell migration and tube formation in HUVEC. In addition, we found that A549 tumorigenesis was reduced in a xenograft mouse model by following the administration of the anti-gC1qR antibody. With these data, we can conclude that the antibody neutralization of cell-surface gC1qR could be a good therapeutic strategy for cancer treatment.
authors.affiliation: Tunneling Nanotube Research Center, Korea University, Seoul, 02841, Korea.|Division of Life Sciences, Korea University, Seoul, 02841, Korea.
authors.forename: Beom-Chan
authors.initials: BC
authors.lastname: Kim
authors.affiliation: Tunneling Nanotube Research Center, Korea University, Seoul, 02841, Korea.|Department of Molecular Medicine, College of Medicine, Inha University, Incheon, 22212, Korea.
authors.forename: Hyun-Jung
authors.initials: HJ
authors.lastname: Hwang
authors.affiliation: Tunneling Nanotube Research Center, Korea University, Seoul, 02841, Korea.|Division of Life Sciences, Korea University, Seoul, 02841, Korea.
authors.forename: Hyoung-Tae
authors.initials: HT
authors.lastname: An
authors.affiliation: Tunneling Nanotube Research Center, Korea University, Seoul, 02841, Korea.|Division of Life Sciences, Korea University, Seoul, 02841, Korea.
authors.forename: Hyun
authors.initials: H
authors.lastname: Lee
authors.affiliation: Tunneling Nanotube Research Center, Korea University, Seoul, 02841, Korea.|Division of Life Sciences, Korea University, Seoul, 02841, Korea.
authors.forename: Jun-Sub
authors.initials: JS
authors.lastname: Park
authors.affiliation: Tunneling Nanotube Research Center, Korea University, Seoul, 02841, Korea.|Division of Life Sciences, Korea University, Seoul, 02841, Korea.
authors.forename: Jin
authors.initials: J
authors.lastname: Hong
authors.affiliation: Tunneling Nanotube Research Center, Korea University, Seoul, 02841, Korea.|Division of Life Sciences, Korea University, Seoul, 02841, Korea.
authors.forename: Jesang
authors.initials: J
authors.lastname: Ko
authors.affiliation: Tunneling Nanotube Research Center, Korea University, Seoul, 02841, Korea.
authors.forename: Chungho
authors.initials: C
authors.lastname: Kim
authors.affiliation: Department of Molecular Medicine, College of Medicine, Inha University, Incheon, 22212, Korea.
authors.forename: Jae-Seon
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Tunneling Nanotube Research Center, Korea University, Seoul, 02841, Korea.|Division of Life Sciences, Korea University, Seoul, 02841, Korea.
authors.forename: Young-Gyu
authors.initials: YG
authors.lastname: Ko
mesh_terms: D000818:Animals; D000906:Antibodies / Q000494:pharmacology*; D063646:Carcinogenesis; D002352:Carrier Proteins / Q000037:antagonists & inhibitors* / Q000276:immunology; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism*; D002465:Cell Movement; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D005260:Female; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D024101:Mitochondrial Proteins / Q000037:antagonists & inhibitors* / Q000276:immunology / Q000378:metabolism; D010766:Phosphorylation; D011554:Pseudopodia / Q000187:drug effects*; D015398:Signal Transduction
chemical_list: D000906:Antibodies; C087299:C1QBP protein, human; D002352:Carrier Proteins; D024101:Mitochondrial Proteins
keywords: antibody; cancer; cell migration; gC1qR; lamellipodia