title: Comparative Transcriptomic Analyses by RNA-seq to Elucidate Differentially Expressed Genes in the Muscle of Korean Thoroughbred Horses.
abstract: The athletic abilities of the horse serve as a valuable model to understand the physiology and molecular mechanisms of adaptive responses to exercise. We analyzed differentially expressed genes in triceps brachii muscle tissues collected from Eonjena Taeyang and Jigusang Seryeok Thoroughbred horses and their co-expression networks in a large-scale RNA-sequence dataset comparing expression before and after exercise. High-quality horse transcriptome data were generated, with over 22 million 90-bp pair-end reads. By comparing the annotations, we found that MYH3, MPZ, and PDE8B genes in Eonjena Taeyang and PDE8B and KIF18A genes in Jigusang Seryeok were upregulated before exercise. Notably further, we observed that PPP1R27, NDUFA3, TNC, and ANK1 in Eonjena Taeyang and HIF1A, BDNF, ADRB2, OBSCN, and PER3 in Jigusang Seryeok have shown upregulation at the postexercise period. This investigation suggested that genes responsible for metabolism and oxidative phosphorylations associated with endurance and resistance exercise were highly expressed, whereas genes encoding structural proteins were generally suppressed. The expression profile of racehorses at pre- and postexercise will provide credible reference for further studies on biological effects such as responses to stress and adaption of other Thoroughbred horse, which might be useful for selective breeding for improvement of traits in commercial production.
authors.affiliation: Department of Animal Biotechnology, Faculty of Biotechnology, Jeju National University, Jeju, 690-756, Republic of Korea.
authors.forename: Mrinmoy
authors.initials: M
authors.lastname: Ghosh
authors.affiliation: Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University, Miryang, 627-702, Republic of Korea.
authors.forename: Hyun-Woo
authors.initials: HW
authors.lastname: Cho
authors.affiliation: Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University, Miryang, 627-702, Republic of Korea.
authors.forename: Jeong-Woong
authors.initials: JW
authors.lastname: Park
authors.affiliation: Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University, Miryang, 627-702, Republic of Korea.
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Choi
authors.affiliation: BK21+, Department of Cogno-Mechatronics Engineering, Pusan National University, Busan, 609-735, Republic of Korea.
authors.forename: Young-Hwa
authors.initials: YH
authors.lastname: Chung
authors.affiliation: Sher-e-Kashmir University of Agricultural Sciences and Technology, Jammu, Jammu and Kashmir, India.
authors.forename: Neelesh
authors.initials: N
authors.lastname: Sharma
authors.affiliation: Department of Animal Biotechnology, Faculty of Biotechnology, Jeju National University, Jeju, 690-756, Republic of Korea.
authors.forename: Amit Kumar
authors.initials: AK
authors.lastname: Singh
authors.affiliation: Department of Animal Biotechnology, Faculty of Biotechnology, Jeju National University, Jeju, 690-756, Republic of Korea.
authors.forename: Nam Eun
authors.initials: NE
authors.lastname: Kim
authors.affiliation: Department of Animal Biotechnology, Faculty of Biotechnology, Jeju National University, Jeju, 690-756, Republic of Korea.
authors.forename: Raj Kumar
authors.initials: RK
authors.lastname: Mongre
authors.affiliation: Department of Animal Biotechnology, Faculty of Biotechnology, Jeju National University, Jeju, 690-756, Republic of Korea.
authors.forename: Do
authors.initials: D
authors.lastname: Huynh
authors.affiliation: Department of Animal Biotechnology, Faculty of Biotechnology, Jeju National University, Jeju, 690-756, Republic of Korea.
authors.forename: Zhang Jiao
authors.initials: ZJ
authors.lastname: Jiao
authors.affiliation: Department of Animal Biotechnology, Faculty of Biotechnology, Jeju National University, Jeju, 690-756, Republic of Korea.
authors.forename: Kyoung Tag
authors.initials: KT
authors.lastname: Do
authors.affiliation: Department of Animal Biotechnology, Chonbuk National University, Jeonju, 561-756, Republic of Korea.
authors.forename: Hak-Kyo
authors.initials: HK
authors.lastname: Lee
authors.affiliation: Department of Animal Biotechnology, Chonbuk National University, Jeonju, 561-756, Republic of Korea.
authors.forename: Ki-Duk
authors.initials: KD
authors.lastname: Song
authors.affiliation: Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University, Miryang, 627-702, Republic of Korea. bwcho@pusan.ac.kr.
authors.forename: Byung-Wook
authors.initials: BW
authors.lastname: Cho
authors.affiliation: Department of Animal Biotechnology, Faculty of Biotechnology, Jeju National University, Jeju, 690-756, Republic of Korea. jeongdk21@gmail.com.
authors.forename: DongKee
authors.initials: D
authors.lastname: Jeong
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D063990:Gene Ontology; D016678:Genome; D006736:Horses / Q000235:genetics*; D058977:Molecular Sequence Annotation; D009132:Muscles / Q000378:metabolism*; D010805:Physical Conditioning, Animal; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D017423:Sequence Analysis, RNA / Q000379:methods*
keywords: Differentially expressed genes; Gene ontology; Stress responses; Thoroughbred horse