title: Cutaneous (tPA) and Skeletal (TnI) mRNA as Markers of Aging in Contused Wound.
abstract: Wound age estimation is one of the most important forensic aspects. Troponin I (TnI) and many cytokines, for example, tissue plasminogen activator (tPA), are involved in wound inflammation and healing. Skeletal (TnI) and cutaneous (tPA) mRNA was detected using real-time PCR in 25 female albino rats. They were divided into 5 groups: control and 4 injured groups. Injured groups were sacrificed 1, 6, 24, and 30 h after inflicting contused wound. The expression levels of cutaneous (tPA) were decreased significantly at 1, 6, and 30 h after contusion (71.7%, 30.7 and 16.9%), while the expression levels of skeletal (TnI) were increased significantly at 1 and 6 h post-traumatic, then they gradually decreased until reaching normal levels at 24 h and assumed significantly lower levels at 30 h postcontusion. These results suggested that the determination of cutaneous (tPA) and skeletal (TnI) mRNA levels was useful for wound age estimation.
authors.affiliation: Lecturer of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Cairo University, Cairo, Egypt.
authors.forename: Samah F
authors.initials: SF
authors.lastname: Ibrahim
authors.affiliation: Lecturer of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Cairo University, Cairo, Egypt.
authors.forename: Marwa
authors.initials: M
authors.lastname: Issak
authors.affiliation: Lecturer of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Cairo University, Cairo, Egypt.
authors.forename: Amany A
authors.initials: AA
authors.lastname: Bayoumy
authors.affiliation: Professor Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt.
authors.forename: Dina S
authors.initials: DS
authors.lastname: Abd El-Fatah
mesh_terms: D000818:Animals; D005260:Female; D049429:Forensic Pathology; D018482:Muscle, Skeletal; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D019210:Troponin I / Q000032:analysis*; D014947:Wounds and Injuries / Q000473:pathology*
chemical_list: D012333:RNA, Messenger; D019210:Troponin I
keywords: blunt trauma; contused wound; forensic science; messenger RNA; real-time PCR; wound age estimation