title: Characterization and Genomic Analysis of a Highly Efficient Dibutyl Phthalate-Degrading Bacterium Gordonia sp. Strain QH-12.
abstract: A bacterial strain QH-12 isolated from activated sludge was identified as Gordonia sp. based on analysis of 16S rRNA gene sequence and was found to be capable of utilizing dibutyl phthalate (DBP) and other common phthalate esters (PAEs) as the sole carbon and energy source. The degradation kinetics of DBP under different concentrations by the strain QH-12 fit well with the modified Gompertz model (R² > 0.98). However, strain QH-12 could not utilize the major intermediate product phthalate (phthalic acid; PA) as the sole carbon and energy source, and only a little amount of PA was detected. The QH-12 genome analysis revealed the presence of putative hydrolase/esterase genes involved in PAEs-degradation but no phthalic acid catabolic gene cluster was found, suggesting that a novel degradation pathway of PAEs was present in Gordonia sp. QH-12. This information will be valuable for obtaining a more holistic understanding on diverse genetic mechanisms of PAEs-degrading Gordonia sp. strains.
authors.affiliation: CAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. dcjin@rcees.ac.cn.
authors.forename: Decai
authors.initials: D
authors.lastname: Jin
authors.affiliation: CAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. kongxiaozaikeda@163.com.
authors.forename: Xiao
authors.initials: X
authors.lastname: Kong
authors.affiliation: Beijing Key Laboratory of Detection and Control of Spoilage Organisms and Pesticide Residues in Agricultural Products, Beijing University of Agriculture, Beijing 102206, China. huijunliu78@163.com.
authors.forename: Huijun
authors.initials: H
authors.lastname: Liu
authors.affiliation: China Offshore Environmental Service Co., Ltd., Tianjin 300452, China. wangxx200899@163.com.
authors.forename: Xinxin
authors.initials: X
authors.lastname: Wang
authors.affiliation: CAS Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. yedeng@rcees.ac.cn.
authors.forename: Ye
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Beijing Key Laboratory of Detection and Control of Spoilage Organisms and Pesticide Residues in Agricultural Products, Beijing University of Agriculture, Beijing 102206, China. mh_jia@yahoo.com.
authors.forename: Minghong
authors.initials: M
authors.lastname: Jia
authors.affiliation: Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China. yuxy@jaas.ac.cn.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Yu
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D003993:Dibutyl Phthalate / Q000378:metabolism*; D004950:Esterases / Q000235:genetics / Q000378:metabolism; D016680:Genome, Bacterial; D033601:Gordonia Bacterium / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D003993:Dibutyl Phthalate; D004950:Esterases
keywords: Gordonia sp.; biodegradation; genome sequencing; phthalate esters; phthalic acid