title: First Description of the Extended Spectrum-Beta-Lactamase Gene blaCTX-M-109 in Salmonella Grumpensis Strains Isolated from Neonatal Nosocomial Infections in Dakar, Senegal.
abstract: Nosocomial infections are very common in African hospitals, particularly in neonatal units. These infections are most often caused by bacteria such as Escherichia coli, Klebsiella spp and Staphylococcus spp. Salmonella strains are rarely involved in nosocomial infections. Here, we report the first description of S. Grumpensis in neonatal infections in Senegal. Seventeen Salmonella strains were isolated from hospitalized infants' stool samples. The following resistance phenotype was described in strains: AMXRTICRCFR FOXRCFXRCTXRCAZRIMPSATMRNARNORRCIPRTMRGMRTERSXTR. All isolates were susceptible to imipenem, 15 out of 17 produced an extended spectrum ß-lactamase (ESBL). blaOXA-1, blaSHV-1, blaTEM-1, blaCTX-M1 genes were detected in strains 8, 13, 5 and 8, respectively. blaCTX-M1 sequencing revealed the presence of blaCTX-M-109. Thirteen of the 17 Salmonella Grumpensis strains were analyzed by PFGE. These 13 isolates belonged to a single pulsotype and were genotypically identical. This is the first report of neonatal S. Grumpensis infections in Senegal, and the first report of blaCTX-M-109 in the genus Salmonella.
authors.affiliation: Unité de Bactériologie Expérimentale (UBE), Institut Pasteur de Dakar, Dakar, Sénégal.|Laboratoire de Bactériologie-Virologie Centre Hospitalier National d'Enfants Albert Royer de Fann Dakar, Dakar, Sénégal.|Faculté de Médecine, Pharmacie et d'Odontologie Université Cheikh Anta Diop de Dakar, Dakar, Sénégal.
authors.forename: Amadou
authors.initials: A
authors.lastname: Diop
authors.affiliation: Unité de Bactériologie Expérimentale (UBE), Institut Pasteur de Dakar, Dakar, Sénégal.
authors.forename: Bissoume
authors.initials: B
authors.lastname: Sambe-Ba
authors.affiliation: Laboratoire d'Analyses Biologiques et Médicales (LABM), Institut Pasteur de Dakar, Dakar, Sénégal.|Faculté de Médecine, Pharmacie et d'Odontologie Université Cheikh Anta Diop de Dakar, Dakar, Sénégal.
authors.forename: Abdoulaye
authors.initials: A
authors.lastname: Seck
authors.affiliation: Laboratoire de Bactériologie-Virologie Centre Hospitalier National de FANN, Dakar, Dakar, Sénégal.|Faculté de Médecine, Pharmacie et d'Odontologie Université Cheikh Anta Diop de Dakar, Dakar, Sénégal.
authors.forename: Mouhamadou Lamine
authors.initials: ML
authors.lastname: Dia
authors.affiliation: Unité de Bactériologie Expérimentale (UBE), Institut Pasteur de Dakar, Dakar, Sénégal.
authors.forename: Lassina Gadi
authors.initials: LG
authors.lastname: Timbiné
authors.affiliation: Unité de Bactériologie Expérimentale (UBE), Institut Pasteur de Dakar, Dakar, Sénégal.
authors.forename: Aïssatou Ameth
authors.initials: AA
authors.lastname: Niang
authors.affiliation: Laboratoire de Bactériologie-Virologie Centre Hospitalier National d'Enfants Albert Royer de Fann Dakar, Dakar, Sénégal.
authors.forename: El Hadji Momar
authors.initials: el HM
authors.lastname: Ndiaye
authors.affiliation: Laboratoire de Bactériologie-Virologie Centre Hospitalier National d'Enfants Albert Royer de Fann Dakar, Dakar, Sénégal.
authors.forename: Mouhamadou Abdoulaye
authors.initials: MA
authors.lastname: Sonko
authors.affiliation: Unité de Bactériologie Expérimentale (UBE), Institut Pasteur de Dakar, Dakar, Sénégal.
authors.forename: Abdoul Aziz
authors.initials: AA
authors.lastname: Wane
authors.affiliation: Laboratoire d'Analyses Biologiques et Médicales (LABM), Institut Pasteur de Dakar, Dakar, Sénégal.
authors.forename: Raymond
authors.initials: R
authors.lastname: Bercion
authors.affiliation: Unité de Néonatologie, Centre Hospitalier Abass Ndao, Dakar, Sénégal.|Faculté de Médecine, Pharmacie et d'Odontologie Université Cheikh Anta Diop de Dakar, Dakar, Sénégal.
authors.forename: Ousmane
authors.initials: O
authors.lastname: Ndiaye
authors.affiliation: Laboratoire de Bactériologie-Virologie Centre Hospitalier National d'Enfants Albert Royer de Fann Dakar, Dakar, Sénégal.|Faculté de Médecine, Pharmacie et d'Odontologie Université Cheikh Anta Diop de Dakar, Dakar, Sénégal.
authors.forename: Moussa Fafa
authors.initials: MF
authors.lastname: Cissé
authors.affiliation: Unité de Bactériologie Expérimentale (UBE), Institut Pasteur de Dakar, Dakar, Sénégal.|Department de Génie Chimique et Biologie Appliquée, Ecole Supérieure Polytechnique, Université Cheikh Anta Diop de Dakar, Dakar, Sénégal.
authors.forename: Amy
authors.initials: A
authors.lastname: Gassama-Sow
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques; D003428:Cross Infection / Q000382:microbiology; D024881:Drug Resistance, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D010641:Phenotype; D012475:Salmonella / Q000235:genetics*; D012480:Salmonella Infections / Q000382:microbiology*; D012675:Senegal; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; C102789:cephamycinase bla(CMY-1); D001618:beta-Lactamases