title: Acetylcholinesterase (AChE) and heat shock proteins (Hsp70) of gypsy moth (Lymantria dispar L.) larvae in response to long-term fluoranthene exposure.
abstract: Polycyclic aromatic hydrocarbons (PAHs) may affect biochemical and physiological processes in living organisms, thus impairing fitness related traits and influencing their populations. This imposes the need for providing early-warning signals of pollution. Our study aimed to examine changes in the activity of acetylcholinesterase (AChE) and the concentration of heat shock proteins (Hsp70) in homogenates of brain tissues of fifth instar gypsy moth (Lymantria dispar L.) larvae, exposed to the ubiquitous PAH, fluoranthene, supplemented to the rearing diet. Significantly increased activity of AChE in larvae fed on the diets with high fluoranthene concentrations suggests the necessity for elucidation of the role of AChE in these insects when exposed to PAH pollution. Significant induction of Hsp70 in gypsy moth larvae reared on the diets containing low fluoranthene concentrations, indicate that changes in the level of Hsp70 might be useful as an indicator of pollution in this widespread forest species.
authors.affiliation: Department of Insect Physiology and Biochemistry, Institute for Biological Research "Siniša Stanković", University of Belgrade, Despot Stefan Blvd. 142, Belgrade, Serbia. Electronic address: mm1507@ibiss.bg.ac.rs.
authors.forename: Marija
authors.initials: M
authors.lastname: Mrdaković
authors.affiliation: Department of Insect Physiology and Biochemistry, Institute for Biological Research "Siniša Stanković", University of Belgrade, Despot Stefan Blvd. 142, Belgrade, Serbia.
authors.forename: Larisa
authors.initials: L
authors.lastname: Ilijin
authors.affiliation: Department of Insect Physiology and Biochemistry, Institute for Biological Research "Siniša Stanković", University of Belgrade, Despot Stefan Blvd. 142, Belgrade, Serbia.
authors.forename: Milena
authors.initials: M
authors.lastname: Vlahović
authors.affiliation: Department of Insect Physiology and Biochemistry, Institute for Biological Research "Siniša Stanković", University of Belgrade, Despot Stefan Blvd. 142, Belgrade, Serbia.
authors.forename: Dragana
authors.initials: D
authors.lastname: Matić
authors.affiliation: Department of Insect Physiology and Biochemistry, Institute for Biological Research "Siniša Stanković", University of Belgrade, Despot Stefan Blvd. 142, Belgrade, Serbia.
authors.forename: Anja
authors.initials: A
authors.lastname: Gavrilović
authors.affiliation: Department of Insect Physiology and Biochemistry, Institute for Biological Research "Siniša Stanković", University of Belgrade, Despot Stefan Blvd. 142, Belgrade, Serbia.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Mrkonja
authors.affiliation: Department of Insect Physiology and Biochemistry, Institute for Biological Research "Siniša Stanković", University of Belgrade, Despot Stefan Blvd. 142, Belgrade, Serbia.
authors.forename: Vesna
authors.initials: V
authors.lastname: Perić-Mataruga
mesh_terms: D000110:Acetylcholinesterase / Q000187:drug effects*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D001923:Brain Chemistry / Q000187:drug effects; D002800:Cholinesterase Inhibitors; D004032:Diet; D004787:Environmental Pollution; D005449:Fluorenes / Q000008:administration & dosage / Q000633:toxicity*; D018840:HSP70 Heat-Shock Proteins / Q000187:drug effects*; D007814:Larva / Q000737:chemistry* / Q000201:enzymology; D009036:Moths; D006178:Romani People
chemical_list: D002800:Cholinesterase Inhibitors; D005449:Fluorenes; D018840:HSP70 Heat-Shock Proteins; C007738:fluoranthene; D000110:Acetylcholinesterase
keywords: Acetylcholinesterase; Fluoranthene; Heat shock proteins 70; Lymantria dispar L.