title: Effects of the led therapy on the global DNA methylation and the expression of Dnmt1 and Dnmt3a genes in a rat model of skin wound healing.
abstract: The use of light emitting diodes (LED) as a therapeutic resource for wound healing has increased over the last years; however, little is still known about the molecular pathways associated to LED exposure. In the present study, we verified the effects of LED therapy on DNA methylation and expression of the DNA methyltransferase (Dnmt) genes, Dnmt1 and Dnmt3a, in an in vivo model of epithelial wound healing. Male Wistar rats were submitted to epithelial excision in the dorsal region and subsequently distributed within the experimental groups: group 1, animals that received irradiation of 0.8 J/cm(2) of LED (604 nm); group 2, animals that received 1.6 J/cm(2) of LED (604 nm); control (CTL), animals not submitted to therapeutic intervention. LED applications were performed during 7 days, and tissues from the periphery of the wound area were obtained for molecular analysis. The Image-J software was used for analysis of the wound area. DNA methylation was evaluated by ELISA-based method and gene expressions were quantified by real-time PCR. Decrease on global DNA methylation profile was observed in all experimental groups (CTL, 1, and 2) revealing the participation of DNA methylation in the healing process. Significant decrease in the wound area accompanied by increase in the Dnmt3a expression was associated to group 2. Based on our findings, we propose that DNA methylation is an important molecular mechanism associated to wound healing and that irradiation with 1.6 J/cm(2) of LED evokes an increase in the expression of the Dnmt3a that might associates to the efficiency of the epithelial wound healing.
authors.affiliation: Centro de Ciências Biológicas e da Saúde, Universidade Norte do Paraná (UNOPAR), Avenida Paris 675, Jardim Piza, CEP: 86041-140, Londrina, PR, Brasil. mvmgomes@gmail.com.
authors.forename: Marcus Vinícius de Matos
authors.initials: MV
authors.lastname: Gomes
authors.affiliation: Centro de Ciências Biológicas e da Saúde, Universidade Norte do Paraná (UNOPAR), Avenida Paris 675, Jardim Piza, CEP: 86041-140, Londrina, PR, Brasil.
authors.forename: Marcelo Henrique
authors.initials: MH
authors.lastname: Manfredo
authors.affiliation: Centro de Ciências Biológicas e da Saúde, Universidade Norte do Paraná (UNOPAR), Avenida Paris 675, Jardim Piza, CEP: 86041-140, Londrina, PR, Brasil.
authors.forename: Leandro Vaz
authors.initials: LV
authors.lastname: Toffoli
authors.affiliation: Centro de Ciências Biológicas e da Saúde, Universidade Norte do Paraná (UNOPAR), Avenida Paris 675, Jardim Piza, CEP: 86041-140, Londrina, PR, Brasil.
authors.forename: Daniellen Christine
authors.initials: DC
authors.lastname: Castro-Alves
authors.affiliation: Centro de Ciências Biológicas e da Saúde, Universidade Norte do Paraná (UNOPAR), Avenida Paris 675, Jardim Piza, CEP: 86041-140, Londrina, PR, Brasil.
authors.forename: Lucas Magnoni
authors.initials: LM
authors.lastname: do Nascimento
authors.affiliation: Centro de Ciências Biológicas e da Saúde, Universidade Norte do Paraná (UNOPAR), Avenida Paris 675, Jardim Piza, CEP: 86041-140, Londrina, PR, Brasil.
authors.forename: Wyllian Rafael
authors.initials: WR
authors.lastname: da Silva
authors.affiliation: Centro de Ciências Biológicas e da Saúde, Universidade Norte do Paraná (UNOPAR), Avenida Paris 675, Jardim Piza, CEP: 86041-140, Londrina, PR, Brasil.
authors.forename: Roberto Kiyoshi
authors.initials: RK
authors.lastname: Kashimoto
authors.affiliation: Centro de Ciências Biológicas e da Saúde, Universidade Norte do Paraná (UNOPAR), Avenida Paris 675, Jardim Piza, CEP: 86041-140, Londrina, PR, Brasil.
authors.forename: Gelson Marcos
authors.initials: GM
authors.lastname: Rodrigues
authors.affiliation: Departamento de Ciências Fisiológicas, Universidade Estadual de Londrina (UEL), Londrina, Paraná, Brasil.
authors.forename: Viviane Batista
authors.initials: VB
authors.lastname: Estrada
authors.affiliation: Centro de Ciências da Saúde, Universidade Estadual de Londrina (UEL), Londrina, Paraná, Brasil.
authors.forename: Rodrigo Antonio
authors.initials: RA
authors.lastname: Andraus
authors.affiliation: Departamento de Ciências Fisiológicas, Universidade Estadual de Londrina (UEL), Londrina, Paraná, Brasil.
authors.forename: Gislaine Garcia
authors.initials: GG
authors.lastname: Pelosi
mesh_terms: D000818:Animals; D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics* / Q000378:metabolism*; D019175:DNA Methylation / Q000528:radiation effects*; D000090205:DNA Methyltransferase 3A; D004195:Disease Models, Animal; D015971:Gene Expression Regulation, Enzymologic / Q000528:radiation effects*; D053685:Laser Therapy; D008297:Male; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D012867:Skin / Q000473:pathology* / Q000528:radiation effects; D014945:Wound Healing / Q000235:genetics* / Q000528:radiation effects*
chemical_list: D000076123:DNA (Cytosine-5-)-Methyltransferase 1; D004248:DNA (Cytosine-5-)-Methyltransferases; D000090205:DNA Methyltransferase 3A; C000619382:Dnmt1 protein, rat
keywords: DNA methylation; Dnmt1; Dnmt3a; LED therapy; Wound healing