title: Laboratory identification of anaerobic bacteria isolated on Clostridium difficile selective medium.
abstract: Despite increasing interest in the bacterium, the methodology for Clostridium difficile recovery has not yet been standardized. Cycloserine-cefoxitin fructose taurocholate (CCFT) has historically been the most used medium for C. difficile isolation from human, animal, environmental, and food samples, and presumptive identification is usually based on colony morphologies. However, CCFT is not totally selective. This study describes the recovery of 24 bacteria species belonging to 10 different genera other than C. difficile, present in the environment and foods of a retirement establishment that were not inhibited in the C. difficile selective medium. These findings provide insight for further environmental and food studies as well as for the isolation of C. difficile on supplemented CCFT.
authors.affiliation: Food Science Department, FARAH, Faculty of Veterinary Medicine, University of Liège , Liège, Belgium.
authors.forename: Cristina
authors.initials: C
authors.lastname: Rodriguez
authors.affiliation: Food Science Department, FARAH, Faculty of Veterinary Medicine, University of Liège , Liège, Belgium.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Warszawski
authors.affiliation: Food Science Department, FARAH, Faculty of Veterinary Medicine, University of Liège , Liège, Belgium.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Korsak
authors.affiliation: Food Science Department, FARAH, Faculty of Veterinary Medicine, University of Liège , Liège, Belgium.
authors.forename: Bernard
authors.initials: B
authors.lastname: Taminiau
authors.affiliation: Belgian Reference Centre for Clostridium difficile (NRC), Pôle de microbiologiemédicale, UniversitéCatholique de Louvain , Brussels, Belgium.
authors.forename: Johan
authors.initials: J
authors.lastname: Van Broeck
authors.affiliation: Belgian Reference Centre for Clostridium difficile (NRC), Pôle de microbiologiemédicale, UniversitéCatholique de Louvain , Brussels, Belgium.
authors.forename: Michel
authors.initials: M
authors.lastname: Delmée
authors.affiliation: Food Science Department, FARAH, Faculty of Veterinary Medicine, University of Liège , Liège, Belgium.
authors.forename: Georges
authors.initials: G
authors.lastname: Daube
mesh_terms: D001421:Bacteria, Anaerobic / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D001431:Bacteriological Techniques / Q000295:instrumentation / Q000379:methods*; D016360:Clostridioides difficile / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism*; D004783:Environmental Microbiology; D005516:Food Microbiology; D006798:Housing; D006801:Humans
chemical_list: D003470:Culture Media
keywords: 16S ribosomal DNA sequence analysis; bacteria identification; cefotaxime; cycloserine–cefoxitin fructose taurocholate medium