title: [Up-regulation of Notch1 inhibits proliferation and differentiation of osteoclast in vitro].
abstract: OBJECTIVE
  This study aimed to explore the effect of the up-regulation of Notch1 on osteoclastogenesis induced to osteoclasts by receptor activator for nuclear factor-kappaB ligand (RANKL) and macrophage colony-stimulating factors (MCSF) in vitro.
  
  
  METHODS
  The bone marrow stem cells (BMSCs) of Rosa(-notch1) mice were cultured and induced to osteoclasts by RANKL and MCSF. The BMSCs were transfected with the Ad-Cre-green fluorescent protein (GFP) virus or Ad-GFP virus. Total RNA from cells was extracted, and the gene expression levels of Notch1, Notch2, Notch3, Notch4, Deltal, Delta3, Delta4, Jagged1, Hes1, and tartrate resistant acid phosphatase (TRAP) were detected at the defined stage by reverse transcription-polymerase chain reaction (RT-PCR). Osteoclast formation was analyzed by TRAP assay.
  
  
  RESULTS
  The number of TRAP-positive multinuclear cells of the experimental group significantly decreased compared with that of the control group. The mRNA expression levels of Notch1, Notch3, Jagged1, Delta3, and Hesl of the experimental group were significantly higher than those of the control group, whereas the TRAP mRNA expression of the experimental group was significantly lower than that of the control group (P<0.05).
  
  
  CONCLUSIONS
  Up-regulation of Notch1 inhibit osteoclastogenesis of BMSCs induced by RANKL and MCSF in vitro.
authors.forename: Yilin
authors.initials: Y
authors.lastname: Ping
authors.forename: Feng
authors.initials: F
authors.lastname: Lou
authors.forename: Xiao
authors.initials: X
authors.lastname: Yang
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D066298:In Vitro Techniques; D016173:Macrophage Colony-Stimulating Factor; D051379:Mice; D010010:Osteoclasts; D053245:RANK Ligand; D053246:Receptor Activator of Nuclear Factor-kappa B; D051881:Receptor, Notch1 / Q000378:metabolism*; D051883:Receptor, Notch2; D015854:Up-Regulation / Q000502:physiology*
chemical_list: C497761:NOTCH1 protein, human; C497764:NOTCH2 protein, human; C497762:Notch1 protein, mouse; C497765:Notch2 protein, mouse; D053245:RANK Ligand; D053246:Receptor Activator of Nuclear Factor-kappa B; D051881:Receptor, Notch1; D051883:Receptor, Notch2; D016173:Macrophage Colony-Stimulating Factor