title: [Distribution of Pathogenic Bacteria and Its Influence on Expression of BCL-2 and BAX Protein after HSCT in the Patients with Hematological Malignancies].
abstract: OBJECTIVE
  To investigate the distribution of pathogenic bacteria in the patients with hematologic malignancies received hematopoietic stem cell transplantation (HSCT) and its influence on the expression of BCL-2 and BAX proteins.
  
  
  METHODS
  The clinical data of 64 patients with malignant lymphoma (ML) received auto-HSCT from January 2011 to December 2015 in our hospital were analyzed. On basis of post-treansplant infection, the patients were divided into infection group (36 cases) and non-infection group (28 cases). The distribution of pathogenic bacteria in 2 groups was identified, the T lymphocyte subsets of peripheral blood, expression level of apoptotic proteins and C-reaction protein (CRP) in 2 group were detected.
  
  
  RESULTS
  Thirty-six strains of pathogenic bacteria were isolated from 36 case of hematological malignancy after HSCT, including 24 strains of Gram-negative bacteria (66.67%) with predominamce of klebsiella pneumoniae (19.44%). The periperal blood CD4+ (t=2.637, P<0.01), CD4+/CD8+ ratio (t=8.223, P<0.01), BCL-2 protein (t=5.852, P<0.05), BCL-2/BAX ratio (t=14.56, P<0.01) in infection group were significantly lower than those in non-infection group, while CD8+ (t=2.285, P=<0.01), CRP (t=39.71, P<0.01), BAX level in infection group were higher than those in non-infection group. The pearson correcation analysis showed that the CD4+/CD8+ ratio in infection group positively correlated with BCL-2/BAX ratio (t=0.341, P<0.05), while serum CRP level in infection group negatively correlated with BCL-2/BAX ratio (t=-0.362, P<0.05).
  
  
  CONCLUSIONS
  The pathogenic bacteria infecting ML patients after HSCT were mainly Gram-negative bacteria. The post-transplant infection can promote the expression up-regulation of related inflammatory factors and apoptotic proteins. The pathogens may be involved in cell apoptisis that provides a new strategy to treat the hematologic malignancies.
authors.affiliation: Department of Hematology, Yijishan Hospital of Wannan Medical College, Wuhu 241000, Anhui Province, China.
authors.forename: Gui-Ping
authors.initials: GP
authors.lastname: Su
authors.affiliation: Department of Hematology, Yijishan Hospital of Wannan Medical College, Wuhu 241000, Anhui Province, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Dai
authors.affiliation: Department of Hematology, Yijishan Hospital of Wannan Medical College, Wuhu 241000, Anhui Province, China.
authors.forename: Lai-Quan
authors.initials: LQ
authors.lastname: Huang
authors.affiliation: Department of Hematology, Yijishan Hospital of Wannan Medical College, Wuhu 241000, Anhui Province, China.
authors.forename: Yi-Zhi
authors.initials: YZ
authors.lastname: Jiang
authors.affiliation: Department of Hematology, Anhui Province Hospital, Hefei 230001, Anhui Province, China.
authors.forename: Liang-Quan
authors.initials: LQ
authors.lastname: Geng
authors.affiliation: Department of Hematology, Anhui Province Hospital, Hefei 230001, Anhui Province, China.
authors.forename: Kai-Yang
authors.initials: KY
authors.lastname: Ding
authors.affiliation: Department of Hematology, Yijishan Hospital of Wannan Medical College, Wuhu 241000, Anhui Province, China. E-mail: hdp-9713@163.com.
authors.forename: Dong-Ping
authors.initials: DP
authors.lastname: Huang
mesh_terms: D002097:C-Reactive Protein / Q000032:analysis; D016516:CD4-CD8 Ratio; D006090:Gram-Negative Bacteria / Q000302:isolation & purification*; D019337:Hematologic Neoplasms / Q000378:metabolism / Q000382:microbiology*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D016176:T-Lymphocyte Subsets / Q000166:cytology; D015854:Up-Regulation; D051028:bcl-2-Associated X Protein / Q000378:metabolism*
chemical_list: C494826:BAX protein, human; C000595707:BCL2 protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D002097:C-Reactive Protein