title: The kinetics and protection of the antiviral state induced by recombinant iIFN1a in rainbow trout against infectious hematopoietic necrosis virus.
abstract: The iIFN1a (intracellular IFN-a1), that is one of the IFN-a1 variants, was shown to be functional intracellularly and act as a novel defense against an infectious hematopoietic necrosis virus (IHNV). To determine its antiviral properties, a recombinant iIFN1a was generated in Escherichia coli. Its antiviral activity against IHNV was 1.69×10(7)U/mg in CHSE-214 cells. Additionally, iIFN1a was capable of inducing comparable levels of IRF-1, IRF-2, IFN-I, IFN-γ and Mx transcription in head kidney, spleen and liver tissues at an early time point (6h), that was followed by a rapid decline 24h after induction. The recombinant protein also elicited protection against IHNV in vivo. At 6 and 24h after induction there was 100% protection against the virus, however, at 48 and 72h the protection decreased to 57 and 40%, respectively. The in vivo protection kinetics correlated with the kinetics of gene expression. The results of this study provide details of the antiviral state that was induced by iIFN1a in vivo for the first time. Additionally, this information will facilitate the development of this recombinant protein as a potential anti-viral treatment and/or adjuvant.
authors.affiliation: Department of Aquaculture, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China. Electronic address: caoyongsheng@hrfri.ac.cn.
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Department of Aquaculture, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China. Electronic address: lmxu0917@163.com.
authors.forename: Liming
authors.initials: L
authors.lastname: Xu
authors.affiliation: Research Division, Clear Springs Foods Inc., P.O. Box 712, Buhl, ID 83316, USA. Electronic address: scott.lapatra@clearsprings.com.
authors.forename: Scott E
authors.initials: SE
authors.lastname: LaPatra
authors.affiliation: Department of Aquaculture, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China. Electronic address: zhaojingzhuang@hrfri.ac.cn.
authors.forename: Jingzhuang
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Aquaculture, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China. Electronic address: 446048847@qq.com.
authors.forename: Miao
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Aquaculture, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China. Electronic address: liuhongbai@sina.com.
authors.forename: Hongbai
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Aquaculture, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China. Electronic address: lutongyan@hotmail.com.
authors.forename: Tongyan
authors.initials: T
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China. Electronic address: zhangqy@ihb.ac.cn.
authors.forename: Qiya
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005393:Fish Diseases / Q000276:immunology*; D029142:Infectious hematopoietic necrosis virus; D016898:Interferon-alpha / Q000276:immunology*; D007700:Kinetics; D017686:Oncorhynchus mykiss / Q000276:immunology* / Q000821:virology; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000276:immunology; D018353:Rhabdoviridae Infections / Q000662:veterinary*
chemical_list: D016898:Interferon-alpha; D011994:Recombinant Proteins
keywords: Antiviral state against IHNV in vivo; Prokaryotic expressing; Variant protection; iIFN1a of rainbow trout