title: COMPARATIVE STUDY ON IMMUNOBLOT VERSUS PCR IN DIAGNOSIS OF SCHISTOSOMIASIS MANSONI IN EXPERIMENTAL INFECTED MICE.
abstract: This study compared PCR and Western blot techniques in diagnosis of schistosomiasis mansoni. Forty Swiss albino mice were used, thirty two mice were infected with cercariae of S. mansoni and eight mice were kept uninfected which were used as a control. Blood was obtained from four infected mice weekly beginning from the 1st week to the 8th week post infection. The study found that PCR was positive from the first week post infection, while Western blot technique was positive from the second week post infection. Thus, PCR diagnosed schistosomiasis mansoni earlier than Western blot technique, but both were able to diagnose.
authors.forename: Mousa A M
authors.initials: MA
authors.lastname: Ismail
authors.forename: Wahed Mohammed Ali
authors.initials: WM
authors.lastname: Mousa
authors.forename: Enas Yahia
authors.initials: EY
authors.lastname: Abu-Sarea
authors.forename: Maha M A
authors.initials: MM
authors.lastname: Basyouni
authors.forename: Samah Sayed
authors.initials: SS
authors.lastname: Mohammed
mesh_terms: D000818:Animals; D000947:Antigens, Helminth; D015153:Blotting, Western; D018509:DNA, Helminth; D051379:Mice; D016133:Polymerase Chain Reaction; D012555:Schistosomiasis mansoni / Q000175:diagnosis* / Q000469:parasitology
chemical_list: D000947:Antigens, Helminth; D018509:DNA, Helminth