title: Intramuscular MAPK signaling following high volume and high intensity resistance exercise protocols in trained men.
abstract: OBJECTIVE
  To examine the mitogen-activated protein kinase (MAPK) family of signaling proteins following typical high volume (HV) and high intensity (HI) lower body resistance exercise protocols in resistance-trained men.
  
  
  METHODS
  Ten resistance-trained men (24.7 ± 3.4 year; 90.1 ± 11.3 kg; 176.0 ± 4.9 cm) performed each resistance exercise protocol in a random, counterbalanced order. The HV protocol utilized a load of 70 % 1-RM for sets of 10-12 repetitions with a 1-min rest period length between sets and exercises. The HI protocol utilized a load of 90 % 1-RM for sets of 3-5 repetitions with a 3-min rest period length between sets and exercises. Both protocols included six sets of barbell back squats and four sets of bilateral leg press, bilateral hamstring curls, bilateral leg extensions, and seated calf raises. Fine needle muscle biopsies of the vastus lateralis were completed at baseline (BL) and 1-h post exercise (1H).
  
  
  RESULTS
  No significant differences over time were noted for phosphorylation of MEK1, ERK1/2, p38, MSK1, ATF2, p53, or c-Jun (p > 0.05). No significance between trial interactions was noted for phosphorylation of MAPK signaling proteins, including MEK1, ERK1/2, p38, JNK, MSK1, ATF2, STAT1, p53, c-Jun, or HSP27 (p > 0.05). However, significant time effects were observed for phosphorylation of JNK (p < 0.01), HSP27 (p < 0.01), and STAT1 (p = 0.03). Phosphorylation of JNK, HSP27, and STAT1 was significantly elevated from BL at 1H for both HV and HI.
  
  
  CONCLUSIONS
  HV and HI lower body resistance exercise protocols appear to elicit similar MAPK activation in resistance-trained men.
authors.affiliation: Department of Health Professions, Hofstra University, Hempstead, NY, USA.
authors.forename: Adam M
authors.initials: AM
authors.lastname: Gonzalez
authors.affiliation: Institute of Exercise Physiology and Wellness, Sport and Exercise Science, College of Education and Human Performance, University of Central Florida, P.O. Box 161250, Orlando, FL, 32816-1250, USA. Jay.Hoffman@ucf.edu.
authors.forename: Jay R
authors.initials: JR
authors.lastname: Hoffman
authors.affiliation: Institute of Exercise Physiology and Wellness, Sport and Exercise Science, College of Education and Human Performance, University of Central Florida, P.O. Box 161250, Orlando, FL, 32816-1250, USA.
authors.forename: Jeremy R
authors.initials: JR
authors.lastname: Townsend
authors.affiliation: Institute of Exercise Physiology and Wellness, Sport and Exercise Science, College of Education and Human Performance, University of Central Florida, P.O. Box 161250, Orlando, FL, 32816-1250, USA.
authors.forename: Adam R
authors.initials: AR
authors.lastname: Jajtner
authors.affiliation: Institute of Exercise Physiology and Wellness, Sport and Exercise Science, College of Education and Human Performance, University of Central Florida, P.O. Box 161250, Orlando, FL, 32816-1250, USA.
authors.forename: Carleigh H
authors.initials: CH
authors.lastname: Boone
authors.affiliation: Institute of Exercise Physiology and Wellness, Sport and Exercise Science, College of Education and Human Performance, University of Central Florida, P.O. Box 161250, Orlando, FL, 32816-1250, USA.
authors.forename: Kyle S
authors.initials: KS
authors.lastname: Beyer
authors.affiliation: Institute of Exercise Physiology and Wellness, Sport and Exercise Science, College of Education and Human Performance, University of Central Florida, P.O. Box 161250, Orlando, FL, 32816-1250, USA.
authors.forename: Kayla M
authors.initials: KM
authors.lastname: Baker
authors.affiliation: Institute of Exercise Physiology and Wellness, Sport and Exercise Science, College of Education and Human Performance, University of Central Florida, P.O. Box 161250, Orlando, FL, 32816-1250, USA.
authors.forename: Adam J
authors.initials: AJ
authors.lastname: Wells
authors.affiliation: Institute of Exercise Physiology and Wellness, Sport and Exercise Science, College of Education and Human Performance, University of Central Florida, P.O. Box 161250, Orlando, FL, 32816-1250, USA.
authors.forename: Gerald T
authors.initials: GT
authors.lastname: Mangine
authors.affiliation: Institute of Exercise Physiology and Wellness, Sport and Exercise Science, College of Education and Human Performance, University of Central Florida, P.O. Box 161250, Orlando, FL, 32816-1250, USA.
authors.forename: Edward H
authors.initials: EH
authors.lastname: Robinson
authors.affiliation: Institute of Exercise Physiology and Wellness, Sport and Exercise Science, College of Education and Human Performance, University of Central Florida, P.O. Box 161250, Orlando, FL, 32816-1250, USA.
authors.forename: David D
authors.initials: DD
authors.lastname: Church
authors.affiliation: Institute of Exercise Physiology and Wellness, Sport and Exercise Science, College of Education and Human Performance, University of Central Florida, P.O. Box 161250, Orlando, FL, 32816-1250, USA.|Department of Internal Medicine, College of Medicine, University of Central Florida, Orlando, FL, USA.
authors.forename: Leonardo P
authors.initials: LP
authors.lastname: Oliveira
authors.affiliation: Institute of Exercise Physiology and Wellness, Sport and Exercise Science, College of Education and Human Performance, University of Central Florida, P.O. Box 161250, Orlando, FL, 32816-1250, USA.
authors.forename: David H
authors.initials: DH
authors.lastname: Fukuda
authors.affiliation: Institute of Exercise Physiology and Wellness, Sport and Exercise Science, College of Education and Human Performance, University of Central Florida, P.O. Box 161250, Orlando, FL, 32816-1250, USA.
authors.forename: Jeffrey R
authors.initials: JR
authors.lastname: Stout
mesh_terms: D015444:Exercise / Q000502:physiology; D000072696:High-Intensity Interval Training / Q000379:methods*; D006801:Humans; D007866:Leg / Q000502:physiology; D020935:MAP Kinase Signaling System / Q000502:physiology*; D008297:Male; D020929:Mitogen-Activated Protein Kinase Kinases / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000502:physiology*; D064797:Physical Conditioning, Human / Q000379:methods / Q000502:physiology*; D005082:Physical Exertion / Q000502:physiology; D010809:Physical Fitness / Q000502:physiology; D055070:Resistance Training / Q000379:methods*; D055815:Young Adult
chemical_list: D020929:Mitogen-Activated Protein Kinase Kinases
keywords: Anabolic intramuscular signaling; ERK; JNK; Mitogen-activated protein kinase; p38