title: Antiapoptotic effect of exercise training on ovariectomized rat hearts.
abstract: The purpose of this study was to evaluate the effects of exercise training on cardiac Fas receptor-dependent and mitochondria-dependent apoptotic pathways in ovariectomized rats. Histopathological analysis, TUNEL assay, and Western blotting were performed on the excised hearts from three groups of Sprague-Dawley rats, which were divided into a sham-operated group, a bilaterally ovariectomized group (OVX), and a bilaterally ovariectomized group that underwent treadmill running exercise for 60 min/day, 5 sessions/wk, for 10 wk (OVX-EX). The abnormal myocardial architecture, cardiac trichome-stained fibrosis and cardiac TUNEL-positive apoptotic cells in ovariectomized rats improved after exercise training. The protein levels of tumor necrosis factor-α, tumor necrosis factor receptor 1, Fas ligand, Fas receptors, Fas-associated death domain, activated caspase-8 and activated caspase-3 (Fas receptor-dependent apoptotic pathways), as well as t-Bid, Bad, Bak, Bax, cytosolic cytochrome c, activated caspase-9, and activated caspase-3 (mitochondria-dependent apoptotic pathways) were decreased in the OVX-EX group compared with the OVX group. Exercise training suppressed ovariectomy-induced cardiac Fas receptor-dependent and mitochondria-dependent apoptotic pathways in ovariectomized rat models. These findings might indicate a new therapeutic effect for exercise training to prevent cardiac apoptosis in menopausal or bilaterally oophorectomized women.
authors.affiliation: Graduate Institute of Chinese Medicine Science, China Medical University, Taichung, Taiwan; Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan; Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan;
authors.forename: Chih-Yang
authors.initials: CY
authors.lastname: Huang
authors.affiliation: Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan;
authors.forename: Yi-Yuan
authors.initials: YY
authors.lastname: Lin
authors.affiliation: Department of Physical Therapy, Graduate Institute of Rehabilitation Science, China Medical University, Taichung, Taiwan;
authors.forename: Chih-Chao
authors.initials: CC
authors.lastname: Hsu
authors.affiliation: Department of Psychology, Asia University, Taichung, Taiwan;
authors.forename: Shiu-Min
authors.initials: SM
authors.lastname: Cheng
authors.affiliation: Translational Medicine Research Center, China Medical University Hospital, Taichung, Taiwan; Graduate Institute of Immunology, China Medical University, Taichung, Taiwan; Center for Neuropsychiatry, Department of Neurology, China Medical University Hospital, Taichung, Taiwan;
authors.forename: Woei-Cherng
authors.initials: WC
authors.lastname: Shyu
authors.affiliation: Center of Sleep Medicine, Department of Physical Medicine and Rehabilitation, Chung-Shan Medical University Hospital, Chung-Shan Medical University, Taichung, Taiwan;
authors.forename: Hua
authors.initials: H
authors.lastname: Ting
authors.affiliation: Department of Sports Sciences, University of Taipei, Taipei, Taiwan;
authors.forename: Ai-Lun
authors.initials: AL
authors.lastname: Yang
authors.affiliation: Graduate Institute of Chinese Medicine Science, China Medical University, Taichung, Taiwan;
authors.forename: Tsung-Jung
authors.initials: TJ
authors.lastname: Ho
authors.affiliation: Department of Physical Therapy, Graduate Institute of Rehabilitation Science, China Medical University, Taichung, Taiwan; Department of Occupational Therapy, Asia University, Taichung, Taiwan; and School of Rehabilitation Science, Shanghai University of TCM, Shanghai, China shinda@mail.cmu.edu.tw.
authors.forename: Shin-Da
authors.initials: SD
authors.lastname: Lee
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000276:immunology*; D051017:Apoptosis Regulatory Proteins / Q000276:immunology*; D005260:Female; D008929:Mitochondria, Heart / Q000276:immunology; D017202:Myocardial Ischemia / Q000209:etiology / Q000276:immunology* / Q000517:prevention & control; D009206:Myocardium / Q000276:immunology*; D010052:Ovariectomy / Q000009:adverse effects*; D010805:Physical Conditioning, Animal / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction
chemical_list: D051017:Apoptosis Regulatory Proteins
keywords: cardiac; caspase; cell death; menopause; treadmill training