title: Characteristics and Antitumor Activity of Morchella esculenta Polysaccharide Extracted by Pulsed Electric Field.
abstract: Polysaccharides from Morchella esculenta have been proven to be functional and helpful for humans. The purpose of this study was to investigate the chemical structure and anti-proliferating and antitumor activities of a Morchella esculenta polysaccharide (MEP) extracted by pulsed electric field (PEF) in submerged fermentation. The endo-polysaccharide was separated and purified by column chromatography and Gel permeation chromatography, and analyzed by gas chromatography. The MEP with an average molecular weight of 81,835 Da consisted of xylose, glucose, mannose, rhamnose and galactose at the ratio of 5.4:5.0:6.5:7.8:72.3. Structure of MEP was further analyzed by Fourier-transform infrared spectroscopy and ¹H and (13)C liquid-state nuclear magnetic resonance spectroscopy. Apoptosis tests proved that MEP could inhibit the proliferation and growth of human colon cancer HT-29 cells in a time- and dose-dependent manner within 48 h. This study provides more information on chemical structure of anti-proliferating polysaccharides isolated from Morchella esculenta.
authors.affiliation: College of Food Science and Engineering, Jilin University, Changchun 130000, China. liuchaocarrol@163.com.|School of Food Engineering, Jilin Agriculture Science and Technology College, Jilin 132101, China. liuchaocarrol@163.com.
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.affiliation: College of Food Science and Engineering, Jilin University, Changchun 130000, China. sunyonghaijida@163.com.
authors.forename: Yonghai
authors.initials: Y
authors.lastname: Sun
authors.affiliation: College of Food Science and Engineering, Jilin University, Changchun 130000, China. mianfeiyouxiangmqq@163.com.
authors.forename: Qian
authors.initials: Q
authors.lastname: Mao
authors.affiliation: College of Food Science and Engineering, Jilin University, Changchun 130000, China. gina_guoxiaolei@163.com.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Guo
authors.affiliation: School of Food Engineering, Jilin Agriculture Science and Technology College, Jilin 132101, China. lipeng8098@126.com.
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.affiliation: College of Food Science and Engineering, Jilin University, Changchun 130000, China. m13704323904@163.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Food Science and Engineering, Jilin University, Changchun 130000, China. doctorserena@163.com.
authors.forename: Na
authors.initials: N
authors.lastname: Xu
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology; D001203:Ascomycota / Q000737:chemistry* / Q000254:growth & development; D005591:Chemical Fractionation / Q000379:methods; D055664:Electrochemical Techniques / Q000379:methods; D005285:Fermentation; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000494:pharmacology; D019073:HT29 Cells; D006801:Humans
chemical_list: D000970:Antineoplastic Agents; D062610:Fungal Polysaccharides
keywords: Morchella esculenta; anti-proliferating activity; chemical structure; polysaccharide; pulsed electric field