title: Cloning, molecular characterization, and expression analysis of the unc45 myosin chaperone b(unc45b)gene of grass carp (Ctenopharyngodon idellus).
abstract: Unc45 myosin chaperone b(unc45b)gene is a molecular chaperone that mediates the folding, assembly and accumulation of thick-filament myosin in the formation of sarcomere, which plays an important role in the development of striated muscle and the stability of sarcomere. In this study, the complete cDNA sequence of unc45b gene of grass carp was obtained by rapid amplification of cDNA ends (RACE), and the characteristics of the unc45b protein predicted from gene sequence was analyzed by bioinformatics methods. The differential expression pattern in tissues was also detected by quantitative real-time PCR. The results showed that the full-length of unc45b gene of grass carp is 3163 bp, which contains a 60 bp 5'UTR, a 298 bp 3'UTR, and a 2865 bp open reading frame (ORF) encoding a 934 amino acid peptide. The deduced unc45b protein exhibits a homology of 92, 86, 86 % with the protein of zebrafish (Danio rerio), channel catfish (Ietalurus punctatus) and tilapia (Oreochromis niloticus) respectively, and the protein contains UCS myosin head binding domain and TPR peptide repeat domain. The protein is a hydrophilic and non-secretory protein with a molecular mass and isoeletronic point of 103,699.8 and 7.39 Da. The structural elements of the protein includes α-helixes and loops, and the unc45b gene highly expresses in skeletal muscle and heart in grass carp. This study laid a foundation for further research in explaining the myofibril accumulation in crisped grass carp.
authors.affiliation: The College of Fisheries and Life Science, Shanghai Ocean University, No. 999, Huchenghuan Road, Shanghai, 201306, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Hu
authors.affiliation: The College of Fisheries and Life Science, Shanghai Ocean University, No. 999, Huchenghuan Road, Shanghai, 201306, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Guo
authors.affiliation: The College of Fisheries and Life Science, Shanghai Ocean University, No. 999, Huchenghuan Road, Shanghai, 201306, China.
authors.forename: Wen-Qian
authors.initials: WQ
authors.lastname: Pan
authors.affiliation: The College of Fisheries and Life Science, Shanghai Ocean University, No. 999, Huchenghuan Road, Shanghai, 201306, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Gan
authors.affiliation: The College of Fisheries and Life Science, Shanghai Ocean University, No. 999, Huchenghuan Road, Shanghai, 201306, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wei
authors.affiliation: The College of Fisheries and Life Science, Shanghai Ocean University, No. 999, Huchenghuan Road, Shanghai, 201306, China.
authors.forename: Jun-Peng
authors.initials: JP
authors.lastname: Wang
authors.affiliation: The College of Fisheries and Life Science, Shanghai Ocean University, No. 999, Huchenghuan Road, Shanghai, 201306, China. xjleng@shou.edu.cn.
authors.forename: Xiang-Jun
authors.initials: XJ
authors.lastname: Leng
authors.affiliation: The College of Fisheries and Life Science, Shanghai Ocean University, No. 999, Huchenghuan Road, Shanghai, 201306, China. xqli@shou.edu.cn.|Key Laboratory of Freshwater Fishery Germplasm Resources, Ministry of Agriculture, No. 999, Huchenghuan Road, Shanghai, 201306, China. xqli@shou.edu.cn.|Shanghai Engineering Research Center of Aquaculture, No. 999, Huchenghuan Road, Shanghai, 201306, China. xqli@shou.edu.cn.|Shanghai University Knowledge Service Platform, Shanghai Ocean University Aquatic Animal Breeding Center, No. 999, Huchenghuan Road, Shanghai, 201306, China. xqli@shou.edu.cn.
authors.forename: Xiao-Qin
authors.initials: XQ
authors.lastname: Li
mesh_terms: D000818:Animals; D002347:Carps; D003001:Cloning, Molecular / Q000379:methods*; D024501:Nonmuscle Myosin Type IIB / Q000235:genetics*
chemical_list: D024501:Nonmuscle Myosin Type IIB
keywords: Bioinformatics analysis; Grass carp; RACE; Tissues differential expression; unc45b gene