title: Clathrin modulates vesicle scission, but not invagination shape, in yeast endocytosis.
abstract: In a previous paper (Picco et al., 2015), the dynamic architecture of the protein machinery during clathrin-mediated endocytosis was visualized using a new live imaging and particle tracking method. Here, by combining this approach with correlative light and electron microscopy, we address the role of clathrin in this process. During endocytosis, clathrin forms a cage-like coat around the membrane and associated protein components. There is growing evidence that clathrin does not determine the membrane morphology of the invagination but rather modulates the progression of endocytosis. We investigate how the deletion of clathrin heavy chain impairs the dynamics and the morphology of the endocytic membrane in budding yeast. Our results show that clathrin is not required for elongating or shaping the endocytic membrane invagination. Instead, we find that clathrin contributes to the regularity of vesicle scission and thereby to controlling vesicle size.
authors.affiliation: Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.|Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.|Division of Cell Biology, MRC Laboratory of Molecular Biology, Cambridge, United Kingdom.
authors.forename: Wanda
authors.identifier: 0000-0002-2778-3936
authors.initials: W
authors.lastname: Kukulski
authors.affiliation: Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.|Department of Biochemistry, University of Geneva, Geneva, Switzerland.
authors.forename: Andrea
authors.identifier: 0000-0003-2548-9183
authors.initials: A
authors.lastname: Picco
authors.affiliation: Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
authors.forename: Tanja
authors.initials: T
authors.lastname: Specht
authors.affiliation: Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.|Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
authors.forename: John Ag
authors.initials: JA
authors.lastname: Briggs
authors.affiliation: Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.|Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.|Department of Biochemistry, University of Geneva, Geneva, Switzerland.
authors.forename: Marko
authors.initials: M
authors.lastname: Kaksonen
mesh_terms: D002966:Clathrin / Q000235:genetics / Q000378:metabolism*; D022163:Clathrin-Coated Vesicles / Q000378:metabolism* / Q000648:ultrastructure*; D004705:Endocytosis; D017353:Gene Deletion; D061848:Optical Imaging; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
chemical_list: D002966:Clathrin
keywords: S. cerevisiae; cell biology; clathrin; correlative microscopy; endocytosis; live-imaging