title: Production and characterization of poly(3-hydroxybutyrate) generated by Alcaligenes latus using lactose and whey after acid protein precipitation process.
abstract: Whey after acid protein precipitation was used as substrate for PHB production in orbital shaker using Alcaligenes latus. Statistical analysis determined the most appropriate hydroxide for pH neutralization of whey after protein precipitation among NH4OH, KOH and NaOH 10%w/v. The results were compared to those of commercial lactose. A scale-up test in a 4L bioreactor was done at 35°C, 750rpm, 7L/min air flow, and 6.5 pH. The PHB was characterized through Fourier Transform Infrared Spectroscopy, thermogravimetry and differential scanning calorimetry. NH4OH provided the best results for productivity (p), 0.11g/L.h, and for polymer yield, (YP/S), 1.08g/g. The bioreactor experiment resulted in lower p and YP/S. PHB showed maximum degradation temperature (291°C), melting temperature (169°C), and chemical properties similar to those of standard PHB. The use of whey as a substrate for PHB production did not affect significantly the final product quality.
authors.affiliation: Laboratory of Energy and Bioprocess, Engineering of Processes and Technology Post-Graduate Program, University of Caxias do Sul, 1130, Francisco Getúlio Vargas Street, 95070-560 Caxias do Sul, Rio Grande do Sul, Brazil.
authors.forename: Karina Hammel
authors.initials: KH
authors.lastname: Berwig
authors.affiliation: Laboratory of Energy and Bioprocess, Engineering of Processes and Technology Post-Graduate Program, University of Caxias do Sul, 1130, Francisco Getúlio Vargas Street, 95070-560 Caxias do Sul, Rio Grande do Sul, Brazil.
authors.forename: Camila
authors.initials: C
authors.lastname: Baldasso
authors.affiliation: Laboratory of Energy and Bioprocess, Engineering of Processes and Technology Post-Graduate Program, University of Caxias do Sul, 1130, Francisco Getúlio Vargas Street, 95070-560 Caxias do Sul, Rio Grande do Sul, Brazil. Electronic address: alinedettmer@gmail.com.
authors.forename: Aline
authors.initials: A
authors.lastname: Dettmer
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000378:metabolism; D000143:Acids / Q000737:chemistry / Q000494:pharmacology; D000421:Alcaligenes / Q000378:metabolism*; D019149:Bioreactors; D002152:Calorimetry, Differential Scanning; D011232:Chemical Precipitation / Q000187:drug effects; D006885:Hydroxybutyrates / Q000032:analysis* / Q000378:metabolism*; D007785:Lactose / Q000378:metabolism*; D011091:Polyesters / Q000032:analysis* / Q000378:metabolism*; D011108:Polymers / Q000378:metabolism; D013696:Temperature; D013818:Thermogravimetry; D000067796:Whey / Q000737:chemistry; D000067816:Whey Proteins / Q000737:chemistry / Q000378:metabolism*; D000094122:Polyhydroxybutyrates
chemical_list: D020155:3-Hydroxybutyric Acid; D000143:Acids; D006885:Hydroxybutyrates; D007785:Lactose; D011091:Polyesters; D011108:Polymers; D000067816:Whey Proteins; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates
keywords: Alcaligenes latus; Ammonium hydroxide; Poly(3-hydroxybutyrate); Protein precipitation; Whey