title: [IL-32 mRNA Expression of Bone Marrow Stromal Cells and Its Correlation with Cell Apoptosis in Patients with Myelodysplastic Syndrome].
abstract: OBJECTIVE
  To investigate the IL-32 mRNA expression of bone marrow stromal cells and its correlation with apoptosis of bone marrow mononuclear cells in patients with myelodysplastic syndrome (MDS).
  
  
  METHODS
  Bone marrow samples from 26 MDS patients and 10 iron deficiency anemia (IDA, as control) patients were collected, RT-PCR was used to detect the IL-32 mRNA expression of bone marrow stromal cells, and the apoptosis of bone marrow mononuclear cells was detected by flow cytometry with Annexin V-FITC/PI dowble staining. The born marrow lymphocytes and NK cells were detected by means of direct immunofluorescence labeling whole blood hemolysis and flow cytometry.
  
  
  RESULTS
  IL-32 mRNA expression of bone marrow stromal cells in the MDS patients was significantly higher than that of control group, the IL-32 mRNA expression of bone marrow stromal cells in patients with RA, RAS and RCMD was significantly higher than that in patients with RAEB. There was no obvious difference between RAEB and the control groups. The apoptosis of bone marrow mononuclear cells in MDS group was significantly higher than that in the control group, the apoptosis of bone marrow mononuclear cells in patients with RA, RAS and RCMD was significantly higher than that in RAEB. There was no significant difference between RAEB group and control group. The IL-32 mRNA expression in bone marrow stromal cells significantly correlated with the apoptosis of bone marrow mononuclear cells in MDS patients. The NK cell number in born marrow of MDS patients and the control group had no significant difference.
  
  
  CONCLUSIONS
  The expression of IL-32 mRNA in bone marrow stromal cells significantly relates with the apoptosis of MDS cells, and the secretion of IL-32 by bone marrow stromal cells may be one of the reasons for the apoptosis of MDS bone marrow cells. It is speculated that the abnormal MDS bone marrow microenvironment is involved in the apoptosis of bone marrow cells.
authors.affiliation: Weifang Medical College, Weifang 261000, Shandong Province, China.
authors.forename: Yuan-Yu
authors.initials: YY
authors.lastname: Zhang
authors.affiliation: Department of Hematology of Jinan Central Hospital Affiliated to Shandong University, Jinan 251000, Shandong Province, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Hematology of Jinan Central Hospital Affiliated to Shandong University, Jinan 251000, Shandong Province, China. E-mail: ldq9194@126.com.
authors.forename: Da-Qi
authors.initials: DQ
authors.lastname: Li
authors.affiliation: Department of Hematology of Jinan Central Hospital Affiliated to Shandong University, Jinan 251000, Shandong Province, China.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Shao
authors.affiliation: Department of Hematology of Jinan Central Hospital Affiliated to Shandong University, Jinan 251000, Shandong Province, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.affiliation: Department of Hematology of Jinan Central Hospital Affiliated to Shandong University, Jinan 251000, Shandong Province, China.
authors.forename: Hong-Yu
authors.initials: HY
authors.lastname: Zhao
authors.affiliation: Department of Hematology of Jinan Central Hospital Affiliated to Shandong University, Jinan 251000, Shandong Province, China.
authors.forename: Xue-Bin
authors.initials: XB
authors.lastname: Dong
authors.affiliation: Department of Hematology of Jinan Central Hospital Affiliated to Shandong University, Jinan 251000, Shandong Province, China.
authors.forename: Lin-Ping
authors.initials: LP
authors.lastname: Gu
authors.affiliation: Department of Hematology of Jinan Central Hospital Affiliated to Shandong University, Jinan 251000, Shandong Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
mesh_terms: D017209:Apoptosis; D001854:Bone Marrow Cells / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D007378:Interleukins / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D009190:Myelodysplastic Syndromes / Q000473:pathology*; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: C496971:IL32 protein, human; D007378:Interleukins; D012333:RNA, Messenger