title: Subpopulations in purified platelets adhering on glass.
abstract: Understanding how platelet activation is regulated is important in the context of cardiovascular disorders and their management with antiplatelet therapy. Recent evidence points to different platelet subpopulations performing different functions. In particular, procoagulant and aggregating subpopulations have been reported in the literature in platelets treated with the GPVI agonists. How the formation of platelet subpopulations upon activation is regulated remains unclear. Here, it is shown that procoagulant and aggregating platelet subpopulations arise spontaneously upon adhesion of purified platelets on clean glass surfaces. Calcium ionophore treatment of the adhering platelets resulted in one platelet population expressing both the procoagulant and the adherent population markers phosphatidylserine and the activated form of GPIIb/IIIa, while all of the platelets expressed CD62P independently of the ionophore treatment. Therefore, all platelets have the capacity to express all three activation markers. It is concluded that platelet subpopulations observed in various studies reflect the dynamics of the platelet activation process.
authors.affiliation: Institute of Functional Interfaces (IFG), Karlsruhe Institute of Technology (KIT), Hermann-von Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
authors.forename: Alessia
authors.initials: A
authors.lastname: Donati
authors.affiliation: Biosurfaces, CIC biomaGUNE, Paseo Miramón 182, San Sebastián 20009, Spain and Department of Biochemistry and Molecular Biology, University of the Basque Country, 28940 Leioa, Spain.
authors.forename: Swati
authors.initials: S
authors.lastname: Gupta
authors.affiliation: Institute of Functional Interfaces (IFG), Karlsruhe Institute of Technology (KIT), Hermann-von Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany and Department of Bioengineering, University of Washington, Seattle, Washington 98105.
authors.forename: Ilya
authors.initials: I
authors.lastname: Reviakine
mesh_terms: D000327:Adsorption; D001777:Blood Coagulation; D001792:Blood Platelets / Q000737:chemistry* / Q000145:classification / Q000502:physiology*; D002449:Cell Aggregation; D005898:Glass / Q000737:chemistry*; D006801:Humans; D019007:P-Selectin / Q000032:analysis; D010718:Phosphatidylserines / Q000032:analysis; D015539:Platelet Activation; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000032:analysis
chemical_list: D019007:P-Selectin; D010718:Phosphatidylserines; D019038:Platelet Glycoprotein GPIb-IX Complex; C546937:SELP protein, human