title: Platelet-Rich Plasma-Loaded Poly(d,l-lactide)-Poly(ethylene glycol)-Poly(d,l-lactide) Hydrogel Dressing Promotes Full-Thickness Skin Wound Healing in a Rodent Model.
abstract: Traditional therapeutic methods for skin wounds have many disadvantages, and new wound dressings that can facilitate the healing process are thus urgently needed. Platelet-rich plasma (PRP) contains multiple growth factors (GFs) and shows a significant capacity to heal soft tissue wounds. However, these GFs have a short half-life and deactivate rapidly; we therefore need a sustained delivery system to overcome this shortcoming. In this study, poly(d,l-lactide)-poly(ethylene glycol)-poly(d,l-lactide) (PDLLA-PEG-PDLLA: PLEL) hydrogel was successfully created as delivery vehicle for PRP GFs and was evaluated systematically. PLEL hydrogel was injectable at room temperature and exhibited a smart thermosensitive in situ gel-formation behavior at body temperature. In vitro cell culture showed PRP-loaded PLEL hydrogel (PRP/PLEL) had little cytotoxicity, and promoted EaHy926 proliferation, migration and tube formation; the factor release assay additionally indicated that PLEL realized the controlled release of PRP GFs for as long as 14 days. When employed to treat rodents' full-thickness skin defects, PRP/PLEL showed a significantly better ability to raise the number of both newly formed and mature blood vessels compared to the control, PLEL and PRP groups. Furthermore, the PRP/PLEL-treated group displayed faster wound closure, better reepithelialization and collagen formation. Taken together, PRP/PLEL provides a promising strategy for promoting angiogenesis and skin wound healing, which extends the potential of this dressing for clinical application.
authors.affiliation: Department of Sports Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, China. qml198816@aliyun.com.
authors.forename: Manle
authors.initials: M
authors.lastname: Qiu
authors.affiliation: Department of Sports Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, China. dychen1218@163.com.
authors.forename: Daoyun
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Gastrointestinal Surgery, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, China. scyshenchaoyong@163.com.
authors.forename: Chaoyong
authors.initials: C
authors.lastname: Shen
authors.affiliation: Department of Sports Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, China. shenjidoctor@163.com.
authors.forename: Ji
authors.initials: J
authors.lastname: Shen
authors.affiliation: Department of Sports Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, China. zhkmssj@126.com.
authors.forename: Huakun
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Sports Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, China. heyaohuadoctor@163.com.
authors.forename: Yaohua
authors.initials: Y
authors.lastname: He
mesh_terms: D000818:Animals; D001458:Bandages / Q000009:adverse effects*; D002460:Cell Line; D020100:Hydrogels / Q000009:adverse effects / Q000737:chemistry*; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology; D051379:Mice; D053657:Platelet-Rich Plasma / Q000737:chemistry*; D011091:Polyesters / Q000009:adverse effects / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000009:adverse effects / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000187:drug effects; D014945:Wound Healing
chemical_list: D020100:Hydrogels; D036341:Intercellular Signaling Peptides and Proteins; D011091:Polyesters; C507889:polylactide-polyethylene glycol-polylactide; D011092:Polyethylene Glycols
keywords: PDLLA-PEG-PDLLA; PLEL hydrogel; PRP; angiogenesis; drug-delivery system; wound healing