title: Polydatin possesses notable anti‑osteoporotic activity via regulation of OPG, RANKL and β‑catenin.
abstract: This study was designed to investigate the anti‑osteoporotic activity of polydatin and its possible underlying mechanism. Osteoporosis was induced in mice by ovariectomy (OVX) and the mice were divided into 5 groups: An OVX only group, polydatin groups (10, 20 and 40 mg/kg) and a sham group (n=10/group). After 12 weeks of treatment, body weight, uterine index and the dry weight of thigh‑bones were recorded. In addition, the serum calcium, serum phosphorus, alkaline phosphatase (ALP) and osteoprotegerin (OPG) levels were also determined. Western blot analysis was then conducted to investigate the possible mechanism underlying the effect of polydatin via determining the expression of OPG, receptor activators of nuclear factor‑κB ligand (RANKL) and β‑catenin in the ST2 cell line. The results indicated that intraperitoneal injection of polydatin (10, 20 and 40 mg/kg/day) decreased body weight, and increased uterine index and dry weights of thigh‑bones of ovariectomized mice (P<0.05), and polydatin also significantly increased the serum calcium, phosphorus, ALP and OPG of ovariectomized mice (P<0.05). Results of western blot analysis showed that polydatin upregulated the ratio of OPG/RANKL (P<0.05) and β‑catenin protein in ST2 cells. In conclusion, the results demonstrated that polydatin exhibits anti‑osteoporotic activity via regulating osteoprotegerin, RANKL and β‑catenin.
authors.affiliation: Department of Internal Medicine, Guangdong Province Corps Hospital, Chinese People's Armed Police Forces, Guangzhou, Guangdong 510507, P.R. China.
authors.forename: Qi-Lin
authors.initials: QL
authors.lastname: Zhou
authors.affiliation: Department of Internal Medicine, Guangdong Province Corps Hospital, Chinese People's Armed Police Forces, Guangzhou, Guangdong 510507, P.R. China.
authors.forename: Ru-Zi
authors.initials: RZ
authors.lastname: Qin
authors.affiliation: Department of Internal Medicine, Guangdong Province Corps Hospital, Chinese People's Armed Police Forces, Guangzhou, Guangdong 510507, P.R. China.
authors.forename: Yan-Xin
authors.initials: YX
authors.lastname: Yang
authors.affiliation: Department of Internal Medicine, Guangdong Province Corps Hospital, Chinese People's Armed Police Forces, Guangzhou, Guangdong 510507, P.R. China.
authors.forename: Kun-Bing
authors.initials: KB
authors.lastname: Huang
authors.affiliation: Department of Internal Medicine, Guangdong Province Corps Hospital, Chinese People's Armed Police Forces, Guangzhou, Guangdong 510507, P.R. China.
authors.forename: Xian-Wen
authors.initials: XW
authors.lastname: Yang
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D015415:Biomarkers; D001835:Body Weight / Q000187:drug effects; D001842:Bone and Bones / Q000187:drug effects / Q000378:metabolism; D002118:Calcium / Q000097:blood; D002460:Cell Line; D004195:Disease Models, Animal; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D005960:Glucosides / Q000494:pharmacology*; D051379:Mice; D010024:Osteoporosis / Q000188:drug therapy / Q000209:etiology* / Q000378:metabolism*; D053244:Osteoprotegerin / Q000235:genetics / Q000378:metabolism*; D010052:Ovariectomy; D053245:RANK Ligand / Q000235:genetics / Q000378:metabolism*; D013267:Stilbenes / Q000494:pharmacology*; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D005960:Glucosides; D053244:Osteoprotegerin; D053245:RANK Ligand; D013267:Stilbenes; D051176:beta Catenin; D000469:Alkaline Phosphatase; D002118:Calcium; C058229:polydatin