title: 2D-gel based proteomics unravels neurogenesis and energetic metabolism dysfunction of the olfactory bulb in CUMS rat model.
abstract: Major depression is a devastating psychiatric disease worldwide currently. A reduced olfactory sensitivity in MDD patients was well evidenced. We previously interrogated the mechanism of decreasing hippocampus neurogenesis in CUMS rat model of depression. The Olfactory Bulb (OB) is crucial part of the olfactory system which functions in post-developmental neurogenesis. However, the mechanism of the dysfunction of OB induced by CUMS is still largely unknown. Herein, by using the chronic unpredictable mild stress (CUMS) rat model of depression, differential protein expression between the OB proteomes of CUMS and control group was interrogated through two-dimensional electrophoresis coupling with matrix-assisted laser desorption ionization-time of flight tandem mass spectrometry. Twenty nine differential protein expression was analyzed by Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway over-representation and Ingenuity pathways analysis (IPA). Seven identified differential proteins were selected for Western blotting validation. This study provides insight that neurogenesis and Energy metabolism disorder is involved in OB dysfunction induced by CUMS.
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; Chongqing Key Laboratory of Neurobiology, Chongqing 400016, China; Institute of Neuroscience and the Collaborative Innovation Center for Brain Science, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Cheng
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; Chongqing Key Laboratory of Neurobiology, Chongqing 400016, China; Institute of Neuroscience and the Collaborative Innovation Center for Brain Science, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Neurology, Yongchuan Hospital of Chongqing Medical University, Chongqing 402460, China; Institute of Neuroscience and the Collaborative Innovation Center for Brain Science, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Deyu
authors.initials: D
authors.lastname: Yang
authors.affiliation: Department of Neurology, The Fifth People's Hospital of Chongqing, Chongqing, China.
authors.forename: Yongtao
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Chongqing Key Laboratory of Neurobiology, Chongqing 400016, China; Institute of Neuroscience and the Collaborative Innovation Center for Brain Science, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Chenglong
authors.initials: C
authors.lastname: Rao
authors.affiliation: Chongqing Key Laboratory of Neurobiology, Chongqing 400016, China; Institute of Neuroscience and the Collaborative Innovation Center for Brain Science, Chongqing Medical University, Chongqing 400016, China; Key Laboratory of Laboratory Medical Diagnostics of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Shuxiao
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Yongchuan Hospital of Chongqing Medical University, Chongqing 402460, China; Chongqing Key Laboratory of Neurobiology, Chongqing 400016, China; Institute of Neuroscience and the Collaborative Innovation Center for Brain Science, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Neurology, Yongchuan Hospital of Chongqing Medical University, Chongqing 402460, China; Chongqing Key Laboratory of Neurobiology, Chongqing 400016, China; Institute of Neuroscience and the Collaborative Innovation Center for Brain Science, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Guo
authors.affiliation: Department of Neurology, Yongchuan Hospital of Chongqing Medical University, Chongqing 402460, China; Institute of Neuroscience and the Collaborative Innovation Center for Brain Science, Chongqing Medical University, Chongqing 400016, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Fang
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhu
authors.affiliation: Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Han
authors.affiliation: Department of Neurology, Yongchuan Hospital of Chongqing Medical University, Chongqing 402460, China; Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; Chongqing Key Laboratory of Neurobiology, Chongqing 400016, China; Institute of Neuroscience and the Collaborative Innovation Center for Brain Science, Chongqing Medical University, Chongqing 400016, China. Electronic address: pengxie@cqmu.edu.cn.
authors.forename: Peng
authors.initials: P
authors.lastname: Xie
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000379:methods; D003863:Depression / Q000503:physiopathology; D003865:Major Depressive Disorder / Q000378:metabolism; D004195:Disease Models, Animal; D004734:Energy Metabolism / Q000502:physiology*; D006624:Hippocampus / Q000378:metabolism; D008297:Male; D055495:Neurogenesis / Q000502:physiology*; D009830:Olfactory Bulb / Q000503:physiopathology; D040901:Proteomics / Q000379:methods; D017207:Rats, Sprague-Dawley; D013315:Stress, Psychological / Q000503:physiopathology*
keywords: 2D-gel based proteomics; CUMS rat model; IPA analysis; Major depression; Olfactory bulb