title: Antennal transcriptome and differential expression of olfactory genes in the yellow peach moth, Conogethes punctiferalis (Lepidoptera: Crambidae).
abstract: The yellow peach moth (YPM), Conogethes punctiferalis (Guenée), is a multivoltine insect pest of crops and fruits. Antennal-expressed receptors are important for insects to detect olfactory cues for host finding, mate attraction and oviposition site selection. However, few olfactory related genes were reported in YPM until now. In the present study, we sequenced and characterized the antennal transcriptomes of male and female YPM. In total, 15 putative odorant binding proteins (OBPs), 46 putative odorant receptors (ORs) and 7 putative ionotropic receptors (IRs) were annotated and identified as olfactory-related genes of C. punctiferalis. Further analysis of RT-qPCR revealed that all these olfactory genes are primarily or uniquely expressed in male and female antennae. Among which, 3 OBPs (OBP4, OBP8 and PBP2) and 4 ORs (OR22, OR26, OR44 and OR46) were specially expressed in male antennae, whereas 4 ORs (OR5, OR16, OR25 and OR42) were primarily expressed in female antennae. The predicted protein sequences were compared with homologs in other lepidopteran species and model insects, which showed high sequence homologies between C. punctiferalis and O. furnacalis. Our work allows for further functional studies of pheromone and general odorant detection genes, which might be meaningful targets for pest management.
authors.affiliation: Beijing Key Laboratory for Agricultural Application and New Technique, College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China.
authors.forename: Xiao-Jian
authors.initials: XJ
authors.lastname: Jia
authors.affiliation: College of Forestry, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
authors.forename: Hai-Xiang
authors.initials: HX
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.
authors.forename: Zeng-Guang
authors.initials: ZG
authors.lastname: Yan
authors.affiliation: Beijing Key Laboratory for Agricultural Application and New Technique, College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China.
authors.forename: Min-Zhao
authors.initials: MZ
authors.lastname: Zhang
authors.affiliation: Beijing Key Laboratory for Agricultural Application and New Technique, College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China.
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Wei
authors.affiliation: Beijing Key Laboratory for Agricultural Application and New Technique, College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China.
authors.forename: Xiao-Chun
authors.initials: XC
authors.lastname: Qin
authors.affiliation: College of Forestry, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
authors.forename: Wei-Rong
authors.initials: WR
authors.lastname: Ji
authors.affiliation: Dipartimento di Scienze, Università della Basilicata, Campus Macchia Romana, Via dell'Ateneo Lucano, 10, Potenza 85100, Italy.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Falabella
authors.affiliation: Beijing Key Laboratory for Agricultural Application and New Technique, College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China.
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: Du
mesh_terms: D000818:Animals; D057506:Arthropod Antennae / Q000502:physiology*; D005260:Female; D005786:Gene Expression Regulation; D019476:Insect Proteins / Q000235:genetics; D008297:Male; D009036:Moths / Q000235:genetics / Q000502:physiology; D055696:Olfactory Perception / Q000235:genetics* / Q000502:physiology; D010675:Pheromones / Q000235:genetics; D010802:Phylogeny; D018035:Receptors, Odorant / Q000235:genetics; D012903:Smell / Q000235:genetics* / Q000502:physiology; D059467:Transcriptome / Q000235:genetics*
chemical_list: D019476:Insect Proteins; D010675:Pheromones; D018035:Receptors, Odorant; C045648:odorant-binding protein