title: Interferon Lambda-3 rs12979860 Variants and Response to Pegylated Interferon in Chronic Hepatitis-C Genotype-3.
abstract: OBJECTIVE
  To assess the role of single nucleotide polymorphisms (SNPs) near the interferon lambda-3 (IFNλ3) (formal IL-28B) gene rs12979860 in predicting sustained virologic response (SVR) in hepatitis-C virus genotype-3 (HCV-3).
  
  
  METHODS
  Descriptive, analytical study.
  
  
  METHODS
  Department of Medicine, The Aga Khan University Hospital, Karachi, from July 2012 to June 2014.
  
  
  METHODS
  Patients with HCV-3 were classified as sustained virologic response (SVR), relapsers and non-responders. SNPrs12979860 was determined by PCR-RFLPprotocol. Differences between categorical variables were assessed by chi-square or Fisher's exact test, while those between continuous variables were evaluated using the Mann-Whitney U-test. Binary logistic regression analysis by forward conditional method was performed by using significant variables with p-values less than 0.05 as the criteria for model inclusion.
  
  
  RESULTS
  Out of 115 patients, rs12979860 genotype-CC, CT, TTwas found in 37 (32.2%), 70 (60.9%), and 8 (7%) patients. 72 patients were male with median age of 45 years. Cirrhosis was present in 32 patients. Patients with response failures (no response and relapse, n=36 and 29, respectively) had higher baseline gamma glutamyl transferase (GGT) level (p < 0.001), higher alanine aminotransferase (p=0.027) and cirrhosis (p=0.001) than patients with SVR. Genotype-CC was present in 16/65 in response failures compared to 21/50 who achieved SVR (p=0.048). Rapid virologic response (RVR) (p < 0.001), low GGT(p=0.001) and absence of cirrhosis (p=0.039) were the independent predictive factors for SVR. In patients who could not achieve RVR and in patients with cirrhosis, SVR was seen more in with genotype-CC (p=0.007 and 0.038).
  
  
  CONCLUSIONS
  In patients infected with HCV-3, IFNλ3 rs12979860, SNPhas less impact on SVR.
authors.affiliation: Department of Medicine, The Aga Khan University Hospital, Karachi.
authors.forename: Zaigham
authors.initials: Z
authors.lastname: Abbas
authors.affiliation: Department of Medicine, The Aga Khan University Hospital, Karachi.
authors.forename: Javed
authors.initials: J
authors.lastname: Yakoob
authors.affiliation: Department of Medicine, The Aga Khan University Hospital, Karachi.
authors.forename: Muhammad A
authors.initials: MA
authors.lastname: Umer
authors.affiliation: Department of Medicine, The Aga Khan University Hospital, Karachi.
authors.forename: Ali A
authors.initials: AA
authors.lastname: Azeem
authors.affiliation: Department of Medicine, The Aga Khan University Hospital, Karachi.
authors.forename: Saeed S
authors.initials: SS
authors.lastname: Hamid
authors.affiliation: Department of Medicine, The Aga Khan University Hospital, Karachi.
authors.forename: Wasim
authors.initials: W
authors.lastname: Jafri
mesh_terms: D000328:Adult; D000368:Aged; D000410:Alanine Transaminase / Q000097:blood / Q000235:genetics; D000998:Antiviral Agents / Q000627:therapeutic use*; D005260:Female; D005838:Genotype; D016174:Hepacivirus / Q000235:genetics*; D019698:Hepatitis C, Chronic / Q000188:drug therapy*; D006801:Humans; D016898:Interferon-alpha / Q000627:therapeutic use*; D007372:Interferons; D007378:Interleukins / Q000235:genetics*; D008103:Liver Cirrhosis / Q000821:virology; D008297:Male; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D012367:RNA, Viral / Q000097:blood; D016896:Treatment Outcome; D005723:gamma-Glutamyltransferase / Q000097:blood; D000095022:Interferon Lambda
chemical_list: D000410:Alanine Transaminase; D000998:Antiviral Agents; D016898:Interferon-alpha; D007372:Interferons; D007378:Interleukins; D012367:RNA, Viral; D005723:gamma-Glutamyltransferase; C000624353:interferon-lambda, human; D000095022:Interferon Lambda