title: Matrix Recrystallization for MALDI-MS Imaging of Maize Lipids at High-Spatial Resolution.
abstract: Matrix recrystallization is optimized and applied to improve lipid ion signals in maize embryos and leaves. A systematic study was performed varying solvent and incubation time. During this study, unexpected side reactions were found when methanol was used as a recrystallization solvent, resulting in the formation of a methyl ester of phosphatidic acid. Using an optimum recrystallization condition with isopropanol, there is no apparent delocalization demonstrated with a transmission electron microscopy (TEM) pattern and maize leaf images obtained at 10 μm spatial resolution. Graphical Abstract ᅟ.
authors.affiliation: Ames Laboratory, US Department of Energy, Ames, IA, 50011, USA.|Department of Chemistry, Iowa State University of Science and Technology, Ames, IA, 50011, USA.
authors.forename: Maria Emilia
authors.initials: ME
authors.lastname: Dueñas
authors.affiliation: Ames Laboratory, US Department of Energy, Ames, IA, 50011, USA.
authors.forename: Laura
authors.initials: L
authors.lastname: Carlucci
authors.affiliation: Ames Laboratory, US Department of Energy, Ames, IA, 50011, USA. yjlee@iastate.edu.|Department of Chemistry, Iowa State University of Science and Technology, Ames, IA, 50011, USA. yjlee@iastate.edu.
authors.forename: Young Jin
authors.initials: YJ
authors.lastname: Lee
keywords: High spatial resolution; Lipids; MALDI; Maize; Mass spectrometry imaging; Matrix; Recrystallization