title: Stable Free-Standing Lipid Bilayer Membranes in Norland Optical Adhesive 81 Microchannels.
abstract: We report a simple, cost-effective, and reproducible method to form free-standing lipid bilayer membranes in microdevices made with Norland Optical Adhesive 81 (NOA81). Surface treatment with either alkylsilane or fluoroalkylsilane enables the self-assembly of stable 1,2-diphytanoyl-sn-glycero-3-phosphocholine 1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC)/1,2-dihexadecanoyl-sn-glycero-3-phosphocholine (DPPC) membranes. Capacitance measurements are used to characterize the lipid bilayer and to follow its formation in real-time. With current recordings, we detect the insertion of single α-hemolysin pores into the bilayer membrane, demonstrating the possibility of using this device for single-channel electrophysiology sensing applications. Optical transparency of the device and vertical position of the lipid bilayer with respect to the microscope focal plane allows easy integration with other single-molecule techniques, such as optical tweezers. Therefore, this method to form long-lived lipid bilayers finds a wide range of applications, from sensing measurements to biophysical studies of lipid bilayers and associated proteins.
authors.affiliation: Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology , 2629 HZ, Delft, The Netherlands.
authors.forename: Victor
authors.initials: V
authors.lastname: Marin
authors.affiliation: Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology , 2629 HZ, Delft, The Netherlands.
authors.forename: Roland
authors.initials: R
authors.lastname: Kieffer
authors.affiliation: Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology , 2629 HZ, Delft, The Netherlands.
authors.forename: Raymond
authors.initials: R
authors.lastname: Padmos
authors.affiliation: Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology , 2629 HZ, Delft, The Netherlands.
authors.forename: Marie-Eve
authors.initials: ME
authors.lastname: Aubin-Tam
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D000269:Adhesives / Q000737:chemistry*; D025802:Electric Capacitance; D006460:Hemolysin Proteins / Q000737:chemistry; D056656:Lab-On-A-Chip Devices; D008051:Lipid Bilayers / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry; D012821:Silanes / Q000737:chemistry
chemical_list: D000269:Adhesives; D006460:Hemolysin Proteins; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D012821:Silanes; D015060:1,2-Dipalmitoylphosphatidylcholine; C021016:1,2-diphytanoylphosphatidylcholine; C017251:1,2-oleoylphosphatidylcholine