title: Porous silicon mass spectrometry as an alternative confirmatory assay for compliance testing of methadone.
abstract: Porous silicon based surface-assisted laser desorption ionization mass spectrometry (pSi SALDI-MS) is an analytical technique well suited for high throughput analysis of low molecular weight compounds from biological samples. A potential application of this technology is the compliance monitoring of opioid addiction programmes, where methadone is used as a pharmacological treatment for drugs such as heroin. Here, we present the detection and quantification of methadone and 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) from water and clinical samples (saliva, urine, and plasma) from opioid dependent participants using pSi SALDI-MS. A one-step solvent phase extraction using chloroform was developed for the detection of methadone from clinical samples for analysis by pSi SALDI-MS. Liquid chromatography-mass spectrometry (LC-MS) was used as a comparative technique for the quantification of methadone from clinical saliva and plasma samples. In all cases, we obtained a good correlation of pSi SALDI-MS and LC-MS results, suggesting that pSi SALDI-MS may be an alternative procedure for high-throughput screening and quantification for application in opioid compliance testing. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Mawson Institute, Australia.|Future Industries Institute, University of South Australia, Adelaide, South Australia, Australia.
authors.forename: Taryn M
authors.initials: TM
authors.lastname: Guinan
authors.affiliation: School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, South Australia, Australia.
authors.forename: Declan
authors.initials: D
authors.lastname: Neldner
authors.affiliation: Forensic Science SA, Adelaide, South Australia, Australia.
authors.forename: Peter
authors.initials: P
authors.lastname: Stockham
authors.affiliation: School of Chemical and Physical Sciences, Flinders University, Bedford Park, South Australia, Australia.
authors.forename: Hilton
authors.initials: H
authors.lastname: Kobus
authors.affiliation: School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, South Australia, Australia.
authors.forename: Christopher B
authors.initials: CB
authors.lastname: Della Vedova
authors.affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Mawson Institute, Australia.|Future Industries Institute, University of South Australia, Adelaide, South Australia, Australia.
authors.forename: Nicolas H
authors.initials: NH
authors.lastname: Voelcker
mesh_terms: D000293:Adolescent; D000328:Adult; D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008691:Methadone / Q000032:analysis / Q000097:blood* / Q000652:urine*; D008875:Middle Aged; D009294:Narcotics / Q000032:analysis / Q000097:blood* / Q000652:urine*; D016062:Porosity; D011759:Pyrrolidines / Q000032:analysis / Q000097:blood* / Q000652:urine*; D015203:Reproducibility of Results; D012463:Saliva / Q000737:chemistry; D012825:Silicon / Q000737:chemistry; D015813:Substance Abuse Detection / Q000379:methods; D014867:Water / Q000032:analysis; D055815:Young Adult
chemical_list: D009294:Narcotics; D011759:Pyrrolidines; D014867:Water; D008691:Methadone; C010724:2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine; D012825:Silicon
keywords: MALDI; SALDI; mass spectrometry; opioid dependence; porous silicon