title: Inhibition of enteroaggregative Escherichia coli cell adhesion in-vitro by designed peptides.
abstract: Enteroaggregative Escherichia coli (EAEC) bears remarkable capacity to adhere the host intestinal mucosal surface and results in acute or persistent childhood diarrhea worldwide. In this study, an attempt has been made to inhibit EAEC cell adherence in-vitro using synthetic peptides. E. coli isolates (n = 54) were isolated from the stool samples of clinically diagnosed pediatric diarrheal patients. 92.8% isolates showed different types of aggregative adherence patterns with HEp-2 cells. AAF-II (Aggregative Adherence Fimbriae-II) EAEC exhibited the maximum ability to form biofilm and intracellular survival. Peptides were designed against the high antigenic epitopic regions of AAF-II adhesin of EAEC O42 using prediction algorithms like BcePred and ProPred software to block the EAEC cell adhesion in-vitro. Peptides P2 (DITITPATNRDVNV) and P3 (MRIKAWGEANHGQL) demonstrated higher inhibition of EAEC cell adhesion than P1 (GMQGSITPAIPLRPG). Interestingly, increasing the pre-incubation time of the peptides with HEp-2 cells from 1 h to 2 h showed the maximum inhibition. The data suggested the potential role of P2 and P3 peptides in successfully blocking the binding of AAF-II EAEC with HEp-2 cell receptors. Hence, the peptides may be efficacious in designing new chemotherapeutic for the management of EAEC mediated diarrhea.
authors.affiliation: Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, India.
authors.forename: Deepika
authors.initials: D
authors.lastname: Gupta
authors.affiliation: Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, India.
authors.forename: Subendu
authors.initials: S
authors.lastname: Sarkar
authors.affiliation: Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, India.
authors.forename: Monica
authors.initials: M
authors.lastname: Sharma
authors.affiliation: Department of Gastroenterology, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, India.
authors.forename: B R
authors.initials: BR
authors.lastname: Thapa
authors.affiliation: Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, India. Electronic address: superoxide14@gmail.com.
authors.forename: Anuradha
authors.initials: A
authors.lastname: Chakraborti
mesh_terms: D018830:Adhesins, Escherichia coli / Q000235:genetics; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects*; D002460:Cell Line; D002648:Child; D019295:Computational Biology; D003967:Diarrhea / Q000382:microbiology; D004847:Epithelial Cells / Q000382:microbiology; D000939:Epitopes / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects* / Q000302:isolation & purification / Q000502:physiology; D004927:Escherichia coli Infections / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D006801:Humans; D010455:Peptides / Q000494:pharmacology*
chemical_list: C108511:AafA protein, E coli; D018830:Adhesins, Escherichia coli; D000900:Anti-Bacterial Agents; D000939:Epitopes; D010455:Peptides
keywords: AAF; Anti adhesion; EAEC; Escherichia coli; HEp-2; Peptide