title: Radioprotective properties of sodium humate in radiation-induced mutagenesis in cultured lymphocytes of thyroid cancer patients.
abstract: To investigate the effect of sodium humate on the level of cytogenetic damage in culture of lymphocytes of patients with thyroid cancer after γ-irradiation.
  
  
  METHODS
  Metaphase analysis of chromosome aberrations in cultured peripheral blood lymphocytes of 10 individuals with thyroid cancer was performed after irradiation of lymphocytes in vitro at a dose of 1 Gy from (137)Cs source at the early G0 phase of cell cycle. Sodium humate was added to cell culture for 30 ± 15 min after phytohemagglutinin stimulation at concentrations of 10 and 100 μg/ml.
  
  
  RESULTS
  Sodium humate exhibited antimutagenic properties. The preparation at a concentration of 10 μg/ml was more effective than at a concentration of 100 μg/ml, reducing the average incidence of radiation-induced chromosome aberrations by 51.88 and 38.77%, respectively. The most pronounced antimutagenic effect of sodium humate was the reduction of the frequency of chromosomal type aberrations, however, such efficiency varied between individual patients with thyroid cancer.
  
  
  CONCLUSIONS
  Sodium humate could be considered as a potential therapeutic modifier of radiation damage.
authors.affiliation: State Institution "National Research Center for Radiation Medicine" of the National Academy of Medical Sciences of Ukraine, Kyiv 04050, Ukraine.
authors.forename: M
authors.initials: M
authors.lastname: Shkarupa
authors.affiliation: State Institution "National Research Center for Radiation Medicine" of the National Academy of Medical Sciences of Ukraine, Kyiv 04050, Ukraine.
authors.forename: S V
authors.initials: SV
authors.lastname: Klymenko
mesh_terms: D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000187:drug effects / Q000528:radiation effects; D005720:Gamma Rays / Q000009:adverse effects*; D006801:Humans; D006812:Humic Substances / Q000032:analysis; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D016296:Mutagenesis / Q000187:drug effects* / Q000528:radiation effects*; D011837:Radiation-Protective Agents / Q000032:analysis / Q000494:pharmacology*; D013964:Thyroid Neoplasms / Q000209:etiology / Q000235:genetics / Q000473:pathology
chemical_list: D006812:Humic Substances; D011837:Radiation-Protective Agents