title: Effects of Environmental Pollutants on MCF-7 Cells: A Metabolic Approach.
abstract: Several environmental pollutants (EPs) have been associated with biological and molecular processes leading to adverse human health effects, including different types of cancer. Nevertheless, the effects exerted on tumor glucose metabolism are unclear. To evaluate the effects on cellular and molecular mechanisms, namely glucose metabolism, MCF-7 cells were exposed to EPs during short- and long-term exposures. The effect of both, organochlorine pesticides and plasticizing agents, on glucose uptake by MCF-7 cells was not dose-dependent and was affected by time of exposure. The ΣHCH and BPA increased glucose uptake after 20 min. Long-term exposure to 250 nM of organochlorine pesticides (p,p'-DDE and ΣHCH) and BPA increased cell proliferation. However, only the organochlorine pesticides were able to increase lactate production, without a concomitant higher glucose uptake or glycolytic enzymes transcription. Given their distinct persistent profiles, the biological significance of their exposure should be considered accordingly. J. Cell. Biochem. 118: 366-375, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Faculty of Medicine, Department of Biochemistry, University of Porto, Porto, 4200-319, Portugal.|Faculty of Medicine, Center for Health Technology and Services Research (CINTESIS), University of Porto, Porto, 4200-450, Portugal.
authors.forename: Sónia
authors.initials: S
authors.lastname: Norberto
authors.affiliation: Faculty of Medicine, Center for Health Technology and Services Research (CINTESIS), University of Porto, Porto, 4200-450, Portugal.|Faculdade de Ciências Médicas, Nutrição e Metabolismo, NOVA Medical School, Universidade NOVA de Lisboa, Lisboa, Portugal.
authors.forename: Conceição
authors.initials: C
authors.lastname: Calhau
authors.affiliation: Faculty of Medicine, Department of Biochemistry, University of Porto, Porto, 4200-319, Portugal.|Faculty of Medicine, Center for Health Technology and Services Research (CINTESIS), University of Porto, Porto, 4200-450, Portugal.
authors.forename: Diogo
authors.initials: D
authors.lastname: Pestana
authors.affiliation: Faculty of Medicine, Department of Biochemistry, University of Porto, Porto, 4200-319, Portugal.|Faculty of Medicine, Center for Health Technology and Services Research (CINTESIS), University of Porto, Porto, 4200-450, Portugal.|Faculty of Nutrition and Food Sciences, University of Porto, Porto, 4200-465, Portugal.|REQUIMTE, Laboratório Associado de Química Verde, Universidade do Porto, Porto, 4169-007, Portugal.
authors.forename: Ana
authors.initials: A
authors.lastname: Faria
mesh_terms: D004785:Environmental Pollutants / Q000633:toxicity*; D006019:Glycolysis / Q000187:drug effects*; D006801:Humans; D006843:Hydrocarbons, Chlorinated / Q000633:toxicity*; D061986:MCF-7 Cells; D010575:Pesticides / Q000633:toxicity*; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects*
chemical_list: D004785:Environmental Pollutants; D006843:Hydrocarbons, Chlorinated; D010575:Pesticides
keywords: ENVIRONMENTAL POLLUTANTS; GLUCOSE METABOLISM; MCF-7; ORGANOCHLORINE PESTICIDES; PLASTICIZING AGENTS