title: Steroid changes adipokine concentration in the blood and bone marrow fluid.
abstract: Our previous study has shown that plasminogen activator inhibitor 1 (PAI-1) gene expression and secretion from bone marrow adipocytes increased markedly with dexamethasone administration. The purpose of the present study was to measure the secretion of various adipokines from human bone marrow and blood, and investigate how adipokine secretion changes in a steroid environment. Human blood and bone marrow fluid were collected from a steroid treatment group and a control group during hip replacement surgery, and an enzyme-linked immunosorbent assay (ELISA) was used to measure the adiponectin, leptin, and PAI-1 levels. Adiponectin and leptin showed no significant differences between bone marrow and blood levels, but PAI-1 was significantly higher in bone marrow. The steroid treatment group had higher levels of leptin and PAI-1 in both the blood and bone marrow than the control group. PAI-1 was present at high concentrations in the bone marrow and increased by steroid treatment. High levels of PAI-1 in bone marrow may influence intraosseous hemodynamics and may induce necrotic bone disorders.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Fukushima
authors.forename: Akira
authors.initials: A
authors.lastname: Hozumi
authors.forename: Masato
authors.initials: M
authors.lastname: Tomita
authors.forename: Akihiko
authors.initials: A
authors.lastname: Yonekura
authors.forename: Noriaki
authors.initials: N
authors.lastname: Miyata
authors.forename: Takashi
authors.initials: T
authors.lastname: Miyamoto
authors.forename: Kenji
authors.initials: K
authors.lastname: Taguchi
authors.forename: Hisataka
authors.initials: H
authors.lastname: Goto
authors.forename: Keiichi
authors.initials: K
authors.lastname: Tsuda
authors.forename: Makoto
authors.initials: M
authors.lastname: Osaki
mesh_terms: D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism; D054392:Adipokines / Q000097:blood / Q000378:metabolism*; D015415:Biomarkers; D001853:Bone Marrow / Q000187:drug effects* / Q000378:metabolism*; D005271:Femur Head Necrosis / Q000209:etiology / Q000378:metabolism; D006801:Humans; D020738:Leptin / Q000097:blood / Q000378:metabolism; D017395:Plasminogen Activator Inhibitor 1 / Q000097:blood / Q000378:metabolism; D013256:Steroids / Q000009:adverse effects / Q000494:pharmacology*
chemical_list: D054392:Adipokines; D015415:Biomarkers; D020738:Leptin; D017395:Plasminogen Activator Inhibitor 1; D013256:Steroids