title: Comparative cytogenetic analysis of four species of Dendropsophus (Hylinae) from the Brazilian Atlantic forest.
abstract: We conducted a cytogenetic study of four hyline frog species (Dendropsophus elegans, D. microps, D. minutus and D. werneri) from southern Brazil. All species had 2n = 30 chromosomes, with interspecific and intraspecific variation in the numbers of metacentric, submetacentric, subtelocentric and telocentric chromosomes. C-banding and fluorochrome staining revealed conservative GC-rich heterochromatin localized in the pericentromeric regions of all species. The location of the nucleolus organizer regions, as confirmed by fluorescent in situ hybridization, differed between species. Telomeric probes detected sites that were restricted to the terminal regions of all chromosomes and no interstitial or centromeric signals were observed. Our study corroborates the generic synapomorphy of 2n = 30 chromosomes for Dendropsophus and adds data that may become useful for future taxonomic revisions and a broader understanding of chromosomal evolution among hylids.
authors.affiliation: Laboratório de História Natural de Anfíbios Brasileiros, Instituto de Biologia, Universidade Estadual de Campinas, 13083-862, Campinas, SP, Brazil.oliveira.snake@gmail.com.
authors.forename: Igor Soares
authors.initials: IS
authors.lastname: De Oliveira
authors.forename: Rafael Bueno
authors.initials: RB
authors.lastname: Noleto
authors.forename: Adriele Karlokoski Cunha De
authors.initials: AK
authors.lastname: Oliveira
authors.forename: Luís Felipe
authors.initials: LF
authors.lastname: Toled
authors.forename: Marta Margarete
authors.initials: MM
authors.lastname: Cestari
mesh_terms: D000818:Animals; D001001:Anura / Q000235:genetics*; D005075:Biological Evolution; D001938:Brazil; D002503:Centromere / Q000648:ultrastructure*; D002871:Chromosome Banding; D005260:Female; D065928:Forests; D006570:Heterochromatin / Q000648:ultrastructure*; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male; D009697:Nucleolus Organizer Region / Q000648:ultrastructure*; D011003:Ploidies; D013045:Species Specificity; D016615:Telomere / Q000648:ultrastructure*
chemical_list: D006570:Heterochromatin