title: Different physicochemical, structural and digestibility characteristics of myofibrillar protein from PSE and normal pork before and after oxidation.
abstract: PSE pork has a weaker texture than normal pork due to the denaturation by endogenous protease. Changes in the nutritional quality and characteristics of myofibrillar protein (MP) from PSE pork have been scarcely documented. MPs were isolated from both PSE (MPP) and normal pork (MPN) and were oxidized for 12h at 4°C by a hydroxyl radical generating system (10μM FeCl3, 100μM ascorbic acid, 0.1, 1, and 10mM H2O2). The MPP had less aggregation and looser structure with higher surface hydrophobicity. The MPP was also less polymeric, as evidenced by intenser bands on SDS-PAGE and fewer carbonyl-NH2 interactions. The MPP particles were of smaller size, caused by the endogenous protease and oxidation, and reached the critical concentration to enhance the solubility. The altered characteristics of MPP enhanced its in vitro digestion rate and overall digestibility. In addition, the in vitro digestion rate of MPN can be enhanced if the oxidative stress is strong enough (10mM H2O2).
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China.
authors.forename: Chenyi
authors.initials: C
authors.lastname: Li
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China; Department of Human Nutrition, The University of Agriculture Peshawar, Pakistan.
authors.forename: Niamat
authors.initials: N
authors.lastname: Ullah
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Guo
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China.
authors.forename: Xuchun
authors.initials: X
authors.lastname: Sun
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China.
authors.forename: Xuejiao
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Meat Processing and Quality Control, Ministry of Education, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Xinglian
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Nutrition, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA.
authors.forename: Robert M
authors.initials: RM
authors.lastname: Hackman
authors.affiliation: Key Laboratory of Meat Processing and Quality Control, Ministry of Education, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: guanghong.zhou@hotmail.com.
authors.forename: Guanghong
authors.initials: G
authors.lastname: Zhou
authors.affiliation: College of Food Science and Engineering, Northwest A&F University, No. 22 Xinong Road, Yangling, Shaanxi 712100, China; Key Laboratory of Meat Processing and Quality Control, Ministry of Education, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: xianchaofeng@hotmail.com.
authors.forename: Xianchao
authors.initials: X
authors.lastname: Feng
mesh_terms: D000818:Animals; D001205:Ascorbic Acid / Q000737:chemistry; D004063:Digestion; D004591:Electrophoresis, Polyacrylamide Gel; D005511:Food Handling; D063427:Food Quality; D006861:Hydrogen Peroxide / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D017665:Hydroxyl Radical / Q000737:chemistry; D008461:Meat Products / Q000032:analysis*; D009124:Muscle Proteins / Q000737:chemistry*; D018482:Muscle, Skeletal / Q000737:chemistry; D010084:Oxidation-Reduction; D000069466:Red Meat / Q000032:analysis*; D012995:Solubility; D013552:Swine
chemical_list: D009124:Muscle Proteins; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide; D001205:Ascorbic Acid
keywords: In vitro digestion; Myofibrillar proteins; Nutritional quality; Oxidation; PSE