title: An improved polymerase chain reaction-restriction fragment length polymorphism assay for the detection of aPON2gene polymorphism.
abstract: In recent research, it has been shown that there have been variants of rs12026 within the paraoxonase 2 (PON2) gene, which have been associated with cardiovascular disease, cerebrovascular disease, diabetes and other diseases. The isochizomers, such as theBsoFI enzyme, required for the detection of this polymorphism are expensive. Therefore, an improved and less expensive polymerase chain reaction (PCR)-restriction fragment length polymorphism method was established for the detection of the single-nucleotide polymorphism rs12026 in the exon 5 of chromosome 7 of the humanPON2gene using the method of amplification-created restriction site. Subsequent to assessing 302 individuals, the genotype frequencies were 68.9% for CC, 29.8% for CG and 1.3% for GG, and the allelic frequencies were 83.8% for C and 16.2% for G. The PCR results were confirmed by DNA sequencing. The χ2test showed that the genotype and allele frequencies ofPON2-148 do not deviate from Hardy-Weinberg equilibrium, and the sequences of amplified products were consistent with the sequence published in GenBank with the exception of a mismatched base.
authors.affiliation: Department of Occupational and Environmental Health, College of Public Health, Zhengzhou University, Zhengzhou, Henan 450001, P.R. China.
authors.forename: Xiaoran
authors.initials: X
authors.lastname: Duan
authors.affiliation: Department of Epidemiology and Biostatistics, College of Public Health, Zhengzhou University, Zhengzhou, Henan 450001, P.R. China.
authors.forename: Yongli
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Occupational and Environmental Health, College of Public Health, Zhengzhou University, Zhengzhou, Henan 450001, P.R. China.
authors.forename: Tuanwei
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Occupational and Environmental Health, College of Public Health, Zhengzhou University, Zhengzhou, Henan 450001, P.R. China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Feng
authors.affiliation: Department of Occupational and Environmental Health, College of Public Health, Zhengzhou University, Zhengzhou, Henan 450001, P.R. China.
authors.forename: W U
authors.initials: WU
authors.lastname: Yao
authors.affiliation: Department of Occupational and Environmental Health, College of Public Health, Zhengzhou University, Zhengzhou, Henan 450001, P.R. China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Yan
authors.affiliation: Department of Occupational and Environmental Health, College of Public Health, Zhengzhou University, Zhengzhou, Henan 450001, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
keywords: BsmAI enzyme; PON2; polymerase chain reaction-restriction fragment length polymorphism; polymorphism; rs12026