title: Generation of KCL034 clinical grade human embryonic stem cell line.
abstract: The KCL034 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment and under current Good Manufacturing Practice (cGMP) standards. Pluripotent state and differentiation potential were confirmed by in vitro assays. The line was also validated for sterility, specific and non-specific human pathogens.
authors.affiliation: Stem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guys' Hospital, London, United Kingdom.
authors.forename: Liani
authors.initials: L
authors.lastname: Devito
authors.affiliation: Stem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guys' Hospital, London, United Kingdom.
authors.forename: Laureen
authors.initials: L
authors.lastname: Jacquet
authors.affiliation: Stem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guys' Hospital, London, United Kingdom.
authors.forename: Anastasia
authors.initials: A
authors.lastname: Petrova
authors.affiliation: Stem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guys' Hospital, London, United Kingdom.
authors.forename: Cristian
authors.initials: C
authors.lastname: Miere
authors.affiliation: Stem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guys' Hospital, London, United Kingdom.
authors.forename: Victoria
authors.initials: V
authors.lastname: Wood
authors.affiliation: Stem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guys' Hospital, London, United Kingdom.
authors.forename: Neli
authors.initials: N
authors.lastname: Kadeva
authors.affiliation: Stem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guys' Hospital, London, United Kingdom.
authors.forename: Glenda
authors.initials: G
authors.lastname: Cornwell
authors.affiliation: Stem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guys' Hospital, London, United Kingdom.
authors.forename: Stefano
authors.initials: S
authors.lastname: Codognotto
authors.affiliation: Stem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guys' Hospital, London, United Kingdom.
authors.forename: Emma
authors.initials: E
authors.lastname: Stephenson
authors.affiliation: Stem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guys' Hospital, London, United Kingdom. Electronic address: dusko.ilic@kcl.ac.uk.
authors.forename: Dusko
authors.initials: D
authors.lastname: Ilic
mesh_terms: D002454:Cell Differentiation; D002460:Cell Line; D055028:Comparative Genomic Hybridization; D004622:Embryo, Mammalian / Q000166:cytology; D005838:Genotype; D006650:Histocompatibility Testing; D000066449:Human Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D008297:Male; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D014157:Transcription Factors