title: [Cloning, expression and identification of gametocyte specific protein Pfgdv1 of Plasmodium falciparum].
abstract: OBJECTIVE
  To clone a gametocyte specific protein Pfgdv1 of Plasmodium falciparum, express and identify recombinant Pfgdvl protein in vitro.
  
  
  METHODS
  PCR was performed to amplify Pfgdv1 from P. falciparum DNA which was got from the patient who was infected with P. falciparum, and the PCR product was inserted into pET28a (+) vector. pET28a-Pfgdv1 recombinant plasmid was constructed and transformed into E. coli host BL21 (DE3+). IPTG was used to induce the recombinant Pfgdv1 protein fused with His tag, and the protein was purified by His-NTA affinity chromatography. The recombinant protein was identified by SDS-PAGE and Western blotting.
  
  
  RESULTS
  The PCR product of Pfgdv1 gene was about 1.65 kb, meeting the expectation of predicted fragment size. The recombinant protein was about 67 kDa, which could be recognized by His-Tag monoclonal antibody.
  
  
  CONCLUSIONS
  The Pfgdv1 gene of P. falciparum is successfully cloned, and the recombinant Pfgdv1 protein is expressed, thereby providing an opportunity for further study on transmission blocking vaccine.
authors.forename: Pang-pang
authors.initials: PP
authors.lastname: Su
authors.forename: Ling-wen
authors.initials: LW
authors.lastname: Meng
authors.forename: Jiang-yan
authors.initials: JY
authors.lastname: Li
authors.forename: Zhi-yong
authors.initials: ZY
authors.lastname: Tao
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.forename: Ji-chen
authors.initials: JC
authors.lastname: Qiao
authors.forename: Xiao-xiao
authors.initials: XX
authors.lastname: Wu
authors.forename: Yun
authors.initials: Y
authors.lastname: Jin
authors.forename: Hao-peng
authors.initials: HP
authors.lastname: Wang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fang
authors.forename: Xue-mei
authors.initials: XM
authors.lastname: Wang
authors.forename: Hui
authors.initials: H
authors.lastname: Xia
mesh_terms: D003001:Cloning, Molecular; D017780:Malaria Vaccines / Q000276:immunology; D010963:Plasmodium falciparum / Q000737:chemistry* / Q000276:immunology; D015800:Protozoan Proteins / Q000235:genetics* / Q000276:immunology; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D014614:Vaccines, Synthetic / Q000276:immunology
chemical_list: D017780:Malaria Vaccines; D015800:Protozoan Proteins; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic