title: miR-124 attenuates Japanese encephalitis virus replication by targeting DNM2.
abstract: Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus that causes acute viral encephalitis in humans. Pigs are important amplifier hosts of JEV. Emerging evidence indicates that host microRNAs (miRNAs) play key roles in modulating viral infection and pathogenesis. However, mechanistic studies delineating the roles of miRNAs in regulating host-JEV interactions remain scarce.
  
  
  
  In this study, we demonstrated that miR-124 inhibited JEV replication in porcine kidney epithelial PK15 cells. Furthermore, using bioinformatics tools, we identified dynamin2 (DNM2), a GTPase responsible for vesicle scission, as a target of miR-124. Small interfering RNA (siRNA) depletion studies inicated that dynamin2 was required for efficient JEV replication. We also demonstrated that upregulation of miR-124 expression corresponded to decreased expression of its target, DNM2, in the JEV-infected PK15 cells.
  
  
  
  Overall, these results suggest the importance of miR-124 in modulating JEV replication and provide a scientific basis for using cellular miRNAs in anti-JEV therapies.
authors.affiliation: College of Animal Science and Technology, Zhejiang A&F University, Lin'an, Zhejiang, 311300, China.
authors.forename: Songbai
authors.initials: S
authors.lastname: Yang
authors.affiliation: College of Animal Science and Technology, Zhejiang A&F University, Lin'an, Zhejiang, 311300, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Pei
authors.affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education & College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Xinyun
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education & College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Shuhong
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education & College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China. zhumengjin@mail.hzau.edu.cn.
authors.forename: Mengjin
authors.initials: M
authors.lastname: Zhu
authors.affiliation: College of Animal Science and Technology, Zhejiang A&F University, Lin'an, Zhejiang, 311300, China. zay503@zafu.edu.cn.
authors.forename: Ayong
authors.initials: A
authors.lastname: Zhao
mesh_terms: D000818:Animals; D034285:Dynamin II / Q000235:genetics* / Q000378:metabolism; D004664:Encephalitis Virus, Japanese / Q000235:genetics / Q000502:physiology*; D004672:Encephalitis, Japanese / Q000235:genetics / Q000378:metabolism / Q000662:veterinary* / Q000821:virology; D054884:Host-Pathogen Interactions; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D013552:Swine; D013553:Swine Diseases / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D014779:Virus Replication
chemical_list: D035683:MicroRNAs; D034285:Dynamin II
keywords: DNM2; JEV; PK15; Porcine; miR-124