title: β-Aminopropionitrile monofumarate induces thoracic aortic dissection in C57BL/6 mice.
abstract: Thoracic aortic dissection (TAD) is a catastrophic disease with high mortality and morbidity, characterized by fragmentation of elastin and loss of smooth muscle cells. However, the underlying pathological mechanisms of this disease remain elusive because there are no appropriate animal models, limiting discovery of effective therapeutic strategies. We treated mice on C57BL/6 and FVB genetic backgrounds with β-aminopropionitrile monofumarate (BAPN), an irreversible inhibitor of lysyl oxidase, for 4 wk, followed by angiotensin II (Ang II) infusion for 24 h. We found that the BAPN plus Ang II treatment induced formation of aortic dissections in 100% of mice on both genetic backgrounds. BAPN without Ang II caused dissections in few FVB mice, but caused 87% of C57BL/6 mice to develop TAD, with 37% dying from rupture of the aortic dissection. Moreover, a lower dose of BAPN induced TAD formation and rupture earlier with fewer effects on body weight. Therefore, we have generated a reliable and convenient TAD model in C57BL/6 mice for studying the pathological process and exploring therapeutic targets of TAD.
authors.affiliation: Beijing Anzhen Hospital, Affiliated to Capital Medical University; Collaborative Innovation Center for Cardiovascular Disorders; The Key Laboratory of Remodeling-Related Cardiovascular Diseases, Ministry of Education; Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing 100029, China.
authors.forename: Weihong
authors.initials: W
authors.lastname: Ren
authors.affiliation: Beijing Anzhen Hospital, Affiliated to Capital Medical University; Collaborative Innovation Center for Cardiovascular Disorders; The Key Laboratory of Remodeling-Related Cardiovascular Diseases, Ministry of Education; Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing 100029, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Beijing Anzhen Hospital, Affiliated to Capital Medical University; Collaborative Innovation Center for Cardiovascular Disorders; The Key Laboratory of Remodeling-Related Cardiovascular Diseases, Ministry of Education; Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing 100029, China.
authors.forename: Xuerui
authors.initials: X
authors.lastname: Wang
authors.affiliation: Beijing Anzhen Hospital, Affiliated to Capital Medical University; Collaborative Innovation Center for Cardiovascular Disorders; The Key Laboratory of Remodeling-Related Cardiovascular Diseases, Ministry of Education; Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing 100029, China.
authors.forename: Lixin
authors.initials: L
authors.lastname: Jia
authors.affiliation: Beijing Anzhen Hospital, Affiliated to Capital Medical University; Collaborative Innovation Center for Cardiovascular Disorders; The Key Laboratory of Remodeling-Related Cardiovascular Diseases, Ministry of Education; Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing 100029, China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Piao
authors.affiliation: Beijing Anzhen Hospital, Affiliated to Capital Medical University; Collaborative Innovation Center for Cardiovascular Disorders; The Key Laboratory of Remodeling-Related Cardiovascular Diseases, Ministry of Education; Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing 100029, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Lan
authors.affiliation: Beijing Anzhen Hospital, Affiliated to Capital Medical University; Collaborative Innovation Center for Cardiovascular Disorders; The Key Laboratory of Remodeling-Related Cardiovascular Diseases, Ministry of Education; Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing 100029, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Du
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism; D000629:Aminopropionitrile / Q000633:toxicity*; D000804:Angiotensin II / Q000633:toxicity; D000818:Animals; D017545:Aortic Aneurysm, Thoracic / Q000209:etiology* / Q000473:pathology; D001794:Blood Pressure / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D000090602:Collagen Type I, alpha 1 Chain; D004195:Disease Models, Animal; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018995:Myosin Heavy Chains / Q000235:genetics / Q000378:metabolism; D010641:Phenotype
chemical_list: D000199:Actins; D024042:Collagen Type I; D000090602:Collagen Type I, alpha 1 Chain; C544013:alpha-smooth muscle actin, mouse; D000804:Angiotensin II; D000629:Aminopropionitrile; D020780:Matrix Metalloproteinase 9; D018995:Myosin Heavy Chains