title: How to Grow a Lung: Applying Principles of Developmental Biology to Generate Lung Lineages from Human Pluripotent Stem Cells.
abstract: The number and severity of diseases affecting human lung development and adult respiratory function has stimulated great interest in new in vitro models to study the human lung. This review summarizes the most recent breakthroughs deriving lung lineages in a dish by directing the differentiation of human pluripotent stem cells. A variety of culturing platforms have been developed, including two-dimensional and three-dimensional (organoid) culture platforms, to derive specific cell types and structures of the lung. These stem cell-derived lung models will further our understanding of human lung development, disease, and regeneration.
authors.affiliation: Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan 48109 USA.
authors.forename: Briana R
authors.initials: BR
authors.lastname: Dye
authors.affiliation: Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48109 USA ; Department of Cell and Molecular Biology, University of Michigan Medical School, Ann Arbor, Michigan 48109 USA.
authors.forename: Alyssa J
authors.initials: AJ
authors.lastname: Miller
authors.affiliation: Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, Michigan 48109 USA ; Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48109 USA ; Department of Cell and Molecular Biology, University of Michigan Medical School, Ann Arbor, Michigan 48109 USA ; Center for Organogenesis, University of Michigan Medical School, Ann Arbor, Michigan 48109 USA.
authors.forename: Jason R
authors.initials: JR
authors.lastname: Spence
keywords: Airway; Alveoli; Directed differentiation; Human pluripotent stem cell; Lung development; Organoid