title: In Vitro Endothelialization Test of Biomaterials Using Immortalized Endothelial Cells.
abstract: Functionalizing biomaterials with peptides or polymers that enhance recruitment of endothelial cells (ECs) can reduce blood coagulation and thrombosis. To assess endothelialization of materials in vitro, primary ECs are generally used, although the characteristics of these cells vary among the donors and change with time in culture. Recently, primary cell lines immortalized by transduction of simian vacuolating virus 40 large T antigen or human telomerase reverse transcriptase have been developed. To determine whether immortalized ECs can substitute for primary ECs in material testing, we investigated endothelialization on biocompatible polymers using three lots of primary human umbilical vein endothelial cells (HUVEC) and immortalized microvascular ECs, TIME-GFP. Attachment to and growth on polymer surfaces were comparable between cell types, but results were more consistent with TIME-GFP. Our findings indicate that TIME-GFP is more suitable for in vitro endothelialization testing of biomaterials.
authors.affiliation: Division of Cell-Based Therapeutic Products, National Institute of Health Sciences, Tokyo, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Kono
authors.affiliation: Division of Medical Devices, National Institute of Health Sciences, Tokyo, Japan.
authors.forename: Hitomi
authors.initials: H
authors.lastname: Hiruma
authors.affiliation: Soft Materials Chemistry, Institute for Materials Chemistry and Engineering, Kyushu University, Fukuoka, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Kobayashi
authors.affiliation: Division of Cell-Based Therapeutic Products, National Institute of Health Sciences, Tokyo, Japan.
authors.forename: Yoji
authors.initials: Y
authors.lastname: Sato
authors.affiliation: Soft Materials Chemistry, Institute for Materials Chemistry and Engineering, Kyushu University, Fukuoka, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Tanaka
authors.affiliation: Division of Cell-Based Therapeutic Products, National Institute of Health Sciences, Tokyo, Japan.
authors.forename: Rumi
authors.initials: R
authors.lastname: Sawada
authors.affiliation: Division of Medical Devices, National Institute of Health Sciences, Tokyo, Japan.
authors.forename: Shingo
authors.initials: S
authors.lastname: Niimi
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion; D002461:Cell Line, Transformed; D042783:Endothelial Cells / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000378:metabolism*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008422:Materials Testing; D010455:Peptides; D011108:Polymers; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D010455:Peptides; D011108:Polymers