title: Administration of polysaccharide from Panax notoginseng prolonged the survival of H22 tumor-bearing mice.
abstract: BACKGROUND
  Polysaccharides from various sources are being considered potential sources for the treatment of liver cancer. The aim of this study was to investigate the impact of polysaccharide isolated from Panax notoginseng (PPN) on the proliferation of H22 liver cancer cells and the survival of the tumor-bearing mice transplanted with H22 cells.
  
  
  METHODS
  Polysaccharide from PPN was added to the culture medium of mouse hepatoma H22 cells at different doses. Cell proliferation was assayed with a standard MTT assay. Survival rates of tumor-bearing mice were recorded. Peripheral blood lymphocytes were assayed by flow cytometry. Serum interleukin-2 levels in peripheral blood were measured by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  Polysaccharide from PPN inhibited the growth of H22 cells and significantly prolonged the survival of tumor-bearing mice. The increase in activated CD4(+) T-cells and the elevation of serum interleukin-2 may contribute to the antitumor activity of PPN.
  
  
  CONCLUSIONS
  PPN has potential antitumor activity for the treatment of liver cancer.
authors.affiliation: Yunnan Key Laboratory of Pharmacology for Natural Products, School of Pharmaceutical Sciences, Kunming Medical University, Kunming, Yunnan, People's Republic of China.
authors.forename: Huaiyu
authors.initials: H
authors.lastname: Li
authors.affiliation: Yunnan Key Laboratory of Pharmacology for Natural Products, School of Pharmaceutical Sciences, Kunming Medical University, Kunming, Yunnan, People's Republic of China; Haiyuan College, Kunming Medical University, Kunming, Yunnan, People's Republic of China.
authors.forename: Longlong
authors.initials: L
authors.lastname: Gu
authors.affiliation: Yunnan Key Laboratory of Pharmacology for Natural Products, School of Pharmaceutical Sciences, Kunming Medical University, Kunming, Yunnan, People's Republic of China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhong
authors.affiliation: Yunnan Key Laboratory of Pharmacology for Natural Products, School of Pharmaceutical Sciences, Kunming Medical University, Kunming, Yunnan, People's Republic of China.
authors.forename: Yajuan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Yunnan Key Laboratory of Pharmacology for Natural Products, School of Pharmaceutical Sciences, Kunming Medical University, Kunming, Yunnan, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Graduate School of Applied and Professional Psychology, Rutgers, The State University of New Jersey, Piscataway, NJ, USA.
authors.forename: Annie R
authors.initials: AR
authors.lastname: Zhang
authors.affiliation: Department of Oncologic Sciences, University of South Alabama, Mobile, AL, USA; Mitchell Cancer Institute, University of South Alabama, Mobile, AL, USA.
authors.forename: Robert W
authors.initials: RW
authors.lastname: Sobol
authors.affiliation: Yunnan Key Laboratory of Pharmacology for Natural Products, School of Pharmaceutical Sciences, Kunming Medical University, Kunming, Yunnan, People's Republic of China; Yunnan Panax notoginseng (Burk) F.H. Chen Biotechnology and Pharmaceutical Engineering Research Center, Kunming, Yunnan, People's Republic of China.
authors.forename: Tong
authors.initials: T
authors.lastname: Chen
authors.affiliation: Department of Oncologic Sciences, University of South Alabama, Mobile, AL, USA; Mitchell Cancer Institute, University of South Alabama, Mobile, AL, USA.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Li
keywords: IL-2; Panax notoginseng; immunotherapy; liver cancer; polysaccharide