title: The localization of SP-B and influences of lipopolysaccharide on it.
abstract: Surfactant protein B (SP-B) is the only essential protein component of lung surfactant, the relationship between localization and function is very close. The localization of rat SP-B and influences of lipopolysaccharide (LPS) on it were investigated.
  
  
  
  The localization of SP-B protein in lung tissue was detected by immunofluorescence after challenged with intratracheal instillation of LPS. The constructed recombinant plasmid pEGFP-N2-SP-B was transfected with CCl-149 cell lines by liposome to explore the localization of SP-B in alveolar type II epithelial cells. Furthermore, the expression and function of SP-B were analyzed with LPS stimulating.
  
  
  
  The proteins SP-B and SP-C showed the same pattern of expression in normal lung, and distributed in the alveolar cavity, LPS can directly affect the expression of SP-B in vivo, contrast to SP-B, the LPS-induced influences on SP-C is little. SP-B had the membrane embedded features and localized on the cell surface of CCL149 in vitro, but was influenced by LPS.
  
  
  
  It is confirmed that the expression and membrane function of SP-B were decreased by stimulating LPS in lung tissue and alveolar type II epithelial cells.
authors.affiliation: State Key Laboratory of Trauma, Burns and Combined Injury, Department 4, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, China. traumazjh@126.com.
authors.forename: W-N
authors.initials: WN
authors.lastname: Wang
authors.forename: J-H
authors.initials: JH
authors.lastname: Zhou
authors.forename: P
authors.initials: P
authors.lastname: Wang
authors.forename: X-J
authors.initials: XJ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D008070:Lipopolysaccharides / Q000494:pharmacology; D008168:Lung / Q000378:metabolism*; D037701:Pulmonary Surfactant-Associated Protein B; D011663:Pulmonary Surfactants; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats
chemical_list: D008070:Lipopolysaccharides; D037701:Pulmonary Surfactant-Associated Protein B; D011663:Pulmonary Surfactants; D012333:RNA, Messenger