title: Delivery of plasmid encoding interleukin-12 gene into hepatocytes by conjugated polyethylenimine-based nanoparticles.
abstract: In the present investigation, polyethylenimine (PEI) was conjugated with succinic anhydride at four substitution degrees and the efficiency of the modified PEI derivatives in transferring the plasmid encoding interleukin-12 gene was evaluated. The results revealed that the conjugated PEI derivatives enhanced the transfection efficiency by up to 3-fold relative to unmodified PEI, with the highest increase occurring at conjugation degrees around 30%. The results demonstrated the ability of the modified PEI derivatives in condensation of the plasmid into the nanoparticles in the size range of approximately 100 nm. Also, the PEI derivatives exhibited substantial decrease in cell-induced toxicity.
authors.affiliation: a Department of Pharmaceutical Biotechnology & Pharmaceutical Sciences Research Center , School of Pharmacy, Shiraz University of Medical Sciences , Shiraz , Iran.
authors.forename: Bahman
authors.initials: B
authors.lastname: Khalvati
authors.affiliation: a Department of Pharmaceutical Biotechnology & Pharmaceutical Sciences Research Center , School of Pharmacy, Shiraz University of Medical Sciences , Shiraz , Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Sheikhsaran
authors.affiliation: b Diagnostic Laboratory , Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences , Shiraz , Iran.
authors.forename: Sedigheh
authors.initials: S
authors.lastname: Sharifzadeh
authors.affiliation: b Diagnostic Laboratory , Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences , Shiraz , Iran.
authors.forename: Tahereh
authors.initials: T
authors.lastname: Kalantari
authors.affiliation: b Diagnostic Laboratory , Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences , Shiraz , Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Behzad Behbahani
authors.affiliation: c Department of Pharmacology and Toxicology , School of Pharmacy, Shiraz University of Medical Sciences , Shiraz , Iran.
authors.forename: Akram
authors.initials: A
authors.lastname: Jamshidzadeh
authors.affiliation: a Department of Pharmaceutical Biotechnology & Pharmaceutical Sciences Research Center , School of Pharmacy, Shiraz University of Medical Sciences , Shiraz , Iran.|d Research Center for Nanotechnology in Drug Delivery, Shiraz University of Medical Sciences , Shiraz , Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Dehshahri
mesh_terms: D004337:Drug Carriers / Q000737:chemistry / Q000633:toxicity; D004912:Erythrocytes / Q000166:cytology; D006461:Hemolysis / Q000187:drug effects; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000378:metabolism*; D006801:Humans; D018664:Interleukin-12 / Q000235:genetics*; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D010957:Plasmids / Q000737:chemistry* / Q000235:genetics*; D011094:Polyethyleneimine / Q000737:chemistry* / Q000633:toxicity; D014162:Transfection
chemical_list: D004337:Drug Carriers; D018664:Interleukin-12; D011094:Polyethyleneimine
keywords: cytotoxicity; interleukin-12; nanoparticle; polyethylenimine