title: Polymorphisms in Glutathione S-Transferase M1, T1, and P1 in Patients with Chronic Periodontitis: A Pilot Study.
abstract: Background. Although the direct cause of chronic periodontitis is bacterial infection, the progression of this disease depends on genetic and environmental factors, and smoking is a known risk factor in the development and severity of the disease. An individual's susceptibility may be influenced by polymorphisms in the glutathione S-transferase genes. These genes encode enzymes that metabolize xenobiotic compounds. The aim of this study was to determine the frequency of GSTM1, GSTT1, and GSTP1 polymorphisms in Mexicans with chronic periodontitis. Methods. 60 Mexicans with chronic periodontitis (30 smokers and 30 nonsmokers) were studied. A peripheral blood sample was taken for subsequent DNA extraction. The genetic material was PCR-amplified followed by restriction fragment length polymorphism with the aim of identifying GST polymorphisms. Results. Polymorphisms in the GSTT1 and GSTP1 genes were not significantly different between the smokers and nonsmokers. However, there were significant differences (P = 0.05) between groups in polymorphisms in the GSTM1 gene. The patients with chronic periodontitis have a higher frequency of null and mutant polymorphisms in GSTM1, GSTT1, and GSTP1 compared with historical data from a healthy Mexican population. Conclusions. The presence of these polymorphisms may be a risk factor for the development of chronic periodontitis.
authors.affiliation: Center of Research and Advanced Studies in Dentistry (CIEAO), Autonomous University of the State of Mexico, 50130 Toluca, MEX, Mexico.
authors.forename: Victor Raul
authors.initials: VR
authors.lastname: Camargo Ortega
authors.affiliation: Center of Research and Advanced Studies in Dentistry (CIEAO), Autonomous University of the State of Mexico, 50130 Toluca, MEX, Mexico.
authors.forename: Leliette Deyanira
authors.initials: LD
authors.lastname: Bravo López
authors.affiliation: Center of Research and Advanced Studies in Dentistry (CIEAO), Autonomous University of the State of Mexico, 50130 Toluca, MEX, Mexico.
authors.forename: Angel
authors.initials: A
authors.lastname: Visoso Salgado
authors.affiliation: Medical Research Center (CICMED), Autonomous University of the State of Mexico, 50130 Toluca, MEX, Mexico.
authors.forename: Fernando
authors.initials: F
authors.lastname: Mejia Sanchez
authors.affiliation: Medical Research Center (CICMED), Autonomous University of the State of Mexico, 50130 Toluca, MEX, Mexico.
authors.forename: Julieta
authors.initials: J
authors.lastname: Castillo Cadena