title: En route from artificial to natural: Evaluation of inhibitors of mannose-specific adhesion of E. coli under flow.
abstract: We investigated the properties of six Escherichia coli adhesion inhibitors under static and under flow conditions. On mannan-covered model substrates and under static conditions, all inhibitors were able to almost completely abolish lectin-mediated E. coli adhesion. On a monolayer of living human microvascular endothelial cells (HMEC-1), the inhibitors reduced adhesion under static conditions as well, but a large fraction of bacteria still managed to adhere even at highest inhibitor concentrations. In contrast, under flow conditions E. coli did not exhibit any adhesion to HMEC-1 not even at inhibitor concentrations where significant adhesion was detected under static conditions. This indicates that the presence of shear stress strongly affects inhibitor properties and must be taken into account when evaluating the potency of bacterial adhesion inhibitors.
authors.affiliation: Department of Physical Chemistry, Ludwig Maximilian University Munich, Butenandtstrasse 11, 81377 Munich, Germany.
authors.forename: Leonhard
authors.initials: L
authors.lastname: Möckl
authors.affiliation: Otto Diels Institute of Organic Chemistry, Christiana Albertina University of Kiel, Otto-Hahn-Platz 3-4, 24098 Kiel, Germany.
authors.forename: Claudia
authors.initials: C
authors.lastname: Fessele
authors.affiliation: Otto Diels Institute of Organic Chemistry, Christiana Albertina University of Kiel, Otto-Hahn-Platz 3-4, 24098 Kiel, Germany.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Despras
authors.affiliation: Department of Physical Chemistry, Ludwig Maximilian University Munich, Butenandtstrasse 11, 81377 Munich, Germany. Electronic address: Christoph.Braeuchle@cup.lmu.de.
authors.forename: Christoph
authors.initials: C
authors.lastname: Bräuchle
authors.affiliation: Otto Diels Institute of Organic Chemistry, Christiana Albertina University of Kiel, Otto-Hahn-Platz 3-4, 24098 Kiel, Germany. Electronic address: tklind@oc.uni-kiel.de.
authors.forename: Thisbe K
authors.initials: TK
authors.lastname: Lindhorst
mesh_terms: D001422:Bacterial Adhesion / Q000187:drug effects / Q000502:physiology*; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology*; D002460:Cell Line; D004730:Endothelium, Vascular / Q000187:drug effects / Q000382:microbiology*; D004926:Escherichia coli / Q000378:metabolism* / Q000502:physiology*; D006801:Humans; D037102:Lectins / Q000378:metabolism; D008351:Mannans / Q000378:metabolism; D008358:Mannose / Q000378:metabolism*; D013314:Stress, Mechanical
chemical_list: D037102:Lectins; D008351:Mannans; D008358:Mannose
keywords: Antiadhesion therapy; Bacterial adhesion; Carbohydrate recognition; Flow conditions; Shear stress