title: Maintenance of Zebrafish Lines at the European Zebrafish Resource Center.
abstract: We have established a European Zebrafish Resource Center (EZRC) at the KIT. This center not only maintains and distributes a large number of existing mutant and transgenic zebrafish lines but also gives zebrafish researchers access to screening services and technologies such as imaging and high-throughput sequencing, provided by the Institute of Toxicology and Genetics (ITG). The EZRC maintains and distributes the stock collection of the Nüsslein-Volhard laboratory, comprising over 2000 publicly released mutations, as frozen sperm samples. Within the framework of the ZF-HEALTH EU project, the EZRC distributes over 10,000 knockout mutations from the Sanger Institute (United Kingdom), as well as over 100 mutant and transgenic lines from other sources. In this article, we detail the measures we have taken to ensure the health of our fish, including hygiene, quarantine, and veterinary inspections.
authors.affiliation: Institute of Toxicology and Genetics, Karlsruhe Institute of Technology (KIT) , Eggenstein-Leopoldshafen, Germany .
authors.forename: Robert
authors.initials: R
authors.lastname: Geisler
authors.affiliation: Institute of Toxicology and Genetics, Karlsruhe Institute of Technology (KIT) , Eggenstein-Leopoldshafen, Germany .
authors.forename: Nadine
authors.initials: N
authors.lastname: Borel
authors.affiliation: Institute of Toxicology and Genetics, Karlsruhe Institute of Technology (KIT) , Eggenstein-Leopoldshafen, Germany .
authors.forename: Marco
authors.initials: M
authors.lastname: Ferg
authors.affiliation: Institute of Toxicology and Genetics, Karlsruhe Institute of Technology (KIT) , Eggenstein-Leopoldshafen, Germany .
authors.forename: Jana Viktoria
authors.initials: JV
authors.lastname: Maier
authors.affiliation: Institute of Toxicology and Genetics, Karlsruhe Institute of Technology (KIT) , Eggenstein-Leopoldshafen, Germany .
authors.forename: Uwe
authors.initials: U
authors.lastname: Strähle
mesh_terms: D000822:Animal Husbandry / Q000379:methods*; D000818:Animals; D030801:Animals, Genetically Modified; D000830:Animals, Laboratory; D017756:Aquaculture / Q000379:methods*; D005060:Europe; D023421:Models, Animal; D015027:Zebrafish