title: Generation of human embryonic stem cell line expressing a red fluorescent protein: BJNhem20-pCAG-tdTomato.
abstract: Human embryonic stem cell line BJNhem20-pCAG-tdTomato was generated using non-viral method. The construct pCAG-tdTomato was transfected using microporation procedure. This fluorescent hESC line can help to study heterogeneity within individual cells in hESC colonies by enabling live tracking of their growth, migration and differentiation properties. This cell line also serves as a resource for additional transgene introduction/knock-out/knock-in generation in a fluorescent background and allows ease of analysis in studies involving cell mixing.
authors.affiliation: Jawaharlal Nehru Centre for Advanced Scientific Research, Bengaluru, India.
authors.forename: Deeti K
authors.initials: DK
authors.lastname: Shetty
authors.affiliation: Jawaharlal Nehru Centre for Advanced Scientific Research, Bengaluru, India; Institute for Stem Cell Biology and Regenerative Medicine, Bengaluru, India.
authors.forename: Maneesha S
authors.initials: MS
authors.lastname: Inamdar
mesh_terms: D002454:Cell Differentiation; D002460:Cell Line; D058732:Embryoid Bodies / Q000166:cytology; D005822:Genetic Vectors / Q000378:metabolism; D000066449:Human Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D059785:Karyotype; D008164:Luminescent Proteins / Q000235:genetics* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014162:Transfection; D000097573:Red Fluorescent Protein
chemical_list: D008164:Luminescent Proteins; D014157:Transcription Factors; D000097573:Red Fluorescent Protein