title: Localization of a Trypanosome Peroxin to the Endoplasmic Reticulum.
abstract: Trypanosoma brucei is the causative agent of diseases that affect 30,000-50,000 people annually. Trypanosoma brucei harbors unique organelles named glycosomes that are essential to parasite survival, which requires growth under fluctuating environmental conditions. The mechanisms that govern the biogenesis of these organelles are poorly understood. Glycosomes are evolutionarily related to peroxisomes, which can proliferate de novo from the endoplasmic reticulum or through the growth and division of existing organelles depending on the organism and environmental conditions. The effect of environment on glycosome biogenesis is unknown. Here, we demonstrate that the glycosome membrane protein, TbPex13.1, is localized to glycosomes when cells are cultured under high glucose conditions and to the endoplasmic reticulum in low glucose conditions. This localization in low glucose was dependent on the presence of a C-terminal tripeptide sequence. Our findings suggest that glycosome biogenesis is influenced by extracellular glucose levels and adds to the growing body of evidence that de novo glycosome biogenesis occurs in trypanosomes. Because the movement of peroxisomal membrane proteins is a hallmark of ER-dependent peroxisome biogenesis, TbPex13.1 may be a useful marker for the study such processes in trypanosomes.
authors.affiliation: Department of Genetics and Biochemistry, Eukaryotic Pathogens Innovation Center, Clemson University, Clemson, South Carolina, 29634.
authors.forename: Sarah T
authors.initials: ST
authors.lastname: Bauer
authors.affiliation: Department of Genetics and Biochemistry, Eukaryotic Pathogens Innovation Center, Clemson University, Clemson, South Carolina, 29634.|Department of Pharmacology, University of Virginia, 409 Lane Road, Charlottesville, Virginia, 22908.
authors.forename: Kelley E
authors.initials: KE
authors.lastname: McQueeney
authors.affiliation: Department of Genetics and Biochemistry, Eukaryotic Pathogens Innovation Center, Clemson University, Clemson, South Carolina, 29634.
authors.forename: Terral
authors.initials: T
authors.lastname: Patel
authors.affiliation: Department of Genetics and Biochemistry, Eukaryotic Pathogens Innovation Center, Clemson University, Clemson, South Carolina, 29634.
authors.forename: Meredith T
authors.initials: MT
authors.lastname: Morris
mesh_terms: D004721:Endoplasmic Reticulum / Q000378:metabolism*; D005947:Glucose / Q000008:administration & dosage / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008830:Microbodies / Q000378:metabolism*; D020675:Peroxisomes / Q000235:genetics / Q000378:metabolism; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D014346:Trypanosoma brucei brucei / Q000235:genetics / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D015800:Protozoan Proteins; D005947:Glucose
keywords: 
  Trypanosoma brucei
  ; Glycosomes; Pex13; peroxisome