title: Detection of cryptic species of Rugopharynx (Nematoda: Strongylida) from the stomachs of Australian macropodid marsupials.
abstract: Sequences of the internal transcribed spacers of nuclear ribosomal DNA (ITS-1 and ITS-2) were determined for species of the genus Rugopharynx and Rugonema labiatum, nematodes from the stomachs of macropodid marsupials. Phylogenetic analyses of the aligned sequence data were conducted. The relationships provided molecular support for all species currently recognised, some of which are based on minor morphological differences and on multilocus enzyme electrophoretic data, but also indicated that additional, cryptic species exist within the genus. In addition, the genus Rugonema is placed as a synonym of Rugopharynx, its sole species becoming Rugopharynx labiatum n. comb. The molecular data provided some insights into the evolution of complex buccal capsule morphologies within the genus, but there was no evidence of co-evolution between the macropodid hosts and their parasites.
authors.affiliation: Department of Biology, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E2, Canada.
authors.forename: Neil B
authors.initials: NB
authors.lastname: Chilton
authors.affiliation: Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC 3010, Australia.
authors.forename: Florence
authors.initials: F
authors.lastname: Huby-Chilton
authors.affiliation: Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC 3010, Australia.
authors.forename: Anson V
authors.initials: AV
authors.lastname: Koehler
authors.affiliation: Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC 3010, Australia.
authors.forename: Robin B
authors.initials: RB
authors.lastname: Gasser
authors.affiliation: Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC 3010, Australia.
authors.forename: Ian
authors.initials: I
authors.lastname: Beveridge
keywords: Cloacininae; Cryptic species; Macropodidae; Phylogeny; Rugopharynx; Strongylida