title: LAMP-3 (Lysosome-Associated Membrane Protein 3) Promotes the Intracellular Proliferation of Salmonella typhimurium.
abstract: Lysosomes are cellular organelles containing diverse classes of catabolic enzymes that are implicated in diverse cellular processes including phagocytosis, autophagy, lipid transport, and aging. Lysosome-associated membrane proteins (LAMP-1 and LAMP-2) are major glycoproteins important for maintaining lysosomal integrity, pH, and catabolism. LAMP-1 and LAMP-2 are constitutively expressed in Salmonella-infected cells and are recruited to Salmonella-containing vacuoles (SCVs) as well as Salmonella-induced filaments (Sifs) that promote the survival and proliferation of the Salmonella. LAMP-3, also known as DC-LAMP/CD208, is a member of the LAMP family of proteins, but its role during Salmonella infection remains unclear. DNA microarray analysis identified LAMP-3 as one of the genes responding to LPS stimulation in THP-1 macrophage cells. Subsequent analyses reveal that LPS and Salmonella induced the expression of LAMP-3 at both the transcriptional and translational levels. Confocal Super resolution N-SIM imaging revealed that LAMP-3, like LAMP-2, shifts its localization from the cell surface to alongside Salmonella. Knockdown of LAMP-3 by specific siRNAs decreased the number of Salmonella recovered from the infected cells. Therefore, we conclude that LAMP-3 is induced by Salmonella infection and recruited to the Salmonella pathogen for intracellular proliferation.
authors.affiliation: Department of Biochemistry, College of Medicine, Chungbuk National University, Cheongju 28644, Korea.
authors.forename: Eun-Ju
authors.initials: EJ
authors.lastname: Lee
authors.affiliation: Department of Biochemistry, College of Medicine, Chungbuk National University, Cheongju 28644, Korea.|Department of Animal Biotechnology, Chonbuk National University, Jeonju 54896, Korea.
authors.forename: Kwan-Sik
authors.initials: KS
authors.lastname: Park
authors.affiliation: Department of Biochemistry, College of Medicine, Chungbuk National University, Cheongju 28644, Korea.
authors.forename: In-Sook
authors.initials: IS
authors.lastname: Jeon
authors.affiliation: Department of Surgery, College of Medicine, Chungbuk National University, Cheongju 28644, Korea.
authors.forename: Jae-Woon
authors.initials: JW
authors.lastname: Choi
authors.affiliation: Department of Surgery, College of Medicine, Chungbuk National University, Cheongju 28644, Korea.
authors.forename: Sang-Jeon
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Department of Microbiology, Chonnam National University Medical School, Gwangju 61186, Korea.
authors.forename: Hyun E
authors.initials: HE
authors.lastname: Choy
authors.affiliation: Department of Animal Biotechnology, Chonbuk National University, Jeonju 54896, Korea.
authors.forename: Ki-Duk
authors.initials: KD
authors.lastname: Song
authors.affiliation: Department of Animal Biotechnology, Chonbuk National University, Jeonju 54896, Korea.
authors.forename: Hak-Kyo
authors.initials: HK
authors.lastname: Lee
authors.affiliation: Department of Biochemistry, College of Medicine, Chungbuk National University, Cheongju 28644, Korea.
authors.forename: Joong-Kook
authors.initials: JK
authors.lastname: Choi
mesh_terms: D002460:Cell Line; D020869:Gene Expression Profiling / Q000379:methods; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051907:Lysosomal Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D020935:MAP Kinase Signaling System; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D012486:Salmonella typhimurium / Q000254:growth & development*; D059666:Lysosomal-Associated Membrane Protein 3
chemical_list: D008070:Lipopolysaccharides; D051907:Lysosomal Membrane Proteins; D016328:NF-kappa B; D009363:Neoplasm Proteins; C114233:LAMP3 protein, human; D059666:Lysosomal-Associated Membrane Protein 3
keywords: LAMP-3; LPS; Salmonella typhimurium; lysosome