title: Apical Secretion of FSTL1 in the Respiratory Epithelium for Normal Lung Development.
abstract: Follistatin-like 1 (FSTL1) is a secreted bone morphogenetic protein (BMP) antagonist, and it plays a crucial role in normal lung development. Deletion of Fstl1 leads to postnatal death in mice due to respiratory failure. To further explore the role of FSTL1 in mouse lung development, we created a transgene SFTPC-Fstl1 allele mouse displaying significant epithelial overexpression of Fstl1 in all stages of lung development. However, epithelial overexpression of Fstl1 did not alter lung morphogenesis, epithelial differentiation and lung function. Moreover, we found that FSTL1 function was blocked by the epithelial polarization, which was reflected by the remarkable apical secretion of FSTL1 and the basolateral BMP signaling. Taken together, this study demonstrates that tightly spatial interaction of FSTL1 and BMP signaling plays an essential role in lung development.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin, 300071, China.
authors.forename: Xiaohe
authors.initials: X
authors.lastname: Li
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin, 300071, China.
authors.forename: Yinshan
authors.initials: Y
authors.lastname: Fang
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin, 300071, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Li
authors.affiliation: Cedars-Sinai Medical Center, Department of Medicine, Los Angeles, CA, 90048, United States of America.
authors.forename: Jiurong
authors.initials: J
authors.lastname: Liang
authors.affiliation: Cedars-Sinai Medical Center, Department of Medicine, Los Angeles, CA, 90048, United States of America.
authors.forename: Dianhua
authors.initials: D
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin, 300071, China.|Cedars-Sinai Medical Center, Department of Medicine, Los Angeles, CA, 90048, United States of America.|School of Pharmaceutical Science, Jiangnan University, Wuxi, Jiangsu, 214122, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Geng
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin, 300071, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Ning
mesh_terms: D000483:Alleles; D000818:Animals; D055415:Bone Morphogenetic Protein 4 / Q000378:metabolism; D018893:Bronchoalveolar Lavage; D003433:Crosses, Genetic; D004848:Epithelium / Q000378:metabolism; D005260:Female; D038702:Follistatin-Related Proteins / Q000378:metabolism*; D008168:Lung / Q000196:embryology* / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D038081:Organogenesis; D010100:Oxygen / Q000378:metabolism; D010641:Phenotype; D020545:Respiratory Mucosa / Q000378:metabolism; D015398:Signal Transduction
chemical_list: C525810:Bmp4 protein, mouse; D055415:Bone Morphogenetic Protein 4; D038702:Follistatin-Related Proteins; C448720:Fstl1 protein, mouse; D010100:Oxygen