title: Simultaneous Determination of Amitraz and its Metabolites in Blood by Support Liquid Extraction Using UPHLC-QTOF.
abstract: This paper describes the use of UPHLC-QTOF for the analysis of pesticide amitraz and its metabolites in human blood. UPHLC-QTOF working in full scan mode simultaneously with a single MS/MS scan at 10 eV was employed to analyze in solid-support liquid-liquid extracts (SLE) of the human blood. Detection and quantification were carried out using full scan data (protonated molecules [M+H]+) while MS/MS data were used for fragment identification only. MS/MS scans were not found to have any negative influence on quantitation under the applied conditions. Verification studies were accomplished at low concentrations (1 ng/mL), and accuracy errors lower than 5 ppm were achieved. The human blood extracts spiked at LOQ and three QC fortification levels produced average recoveries in the range of 79.3-92.5% with relative standard deviations smaller than 10% for all analytes. Limits of detection (LODs) were between 0.1 and 0.5 ng/mL. Finally, the proposed method was successfully applied to a case of human poisoning.
authors.affiliation: Chongqing Key Lab of Catalysis & Functional Organic Molecules, Chongqing Technology and Business University, Chongqing 400067, China School of Resources and Environment, Chongqing Technology and Business University, Chongqing 400067, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Gao
authors.affiliation: Chongqing Institute of Forensic Science, Chongqing 400021, China hao.guo1982@hotmail.com.
authors.forename: Hao
authors.initials: H
authors.lastname: Guo
authors.affiliation: Department of Fiber and Polymer Science, College of Textiles, North Carolina State University, Raleigh, NC 27606, USA.
authors.forename: Wen-Wen
authors.initials: WW
authors.lastname: Zhang
authors.affiliation: Reel Solar Power Inc., San Jose, CA 95131, USA.
authors.forename: Chaokang
authors.initials: C
authors.lastname: Gu
mesh_terms: D002853:Chromatography, Liquid; D053593:Forensic Toxicology; D006801:Humans; D007306:Insecticides / Q000097:blood*; D011041:Poisoning / Q000097:blood / Q000175:diagnosis; D014052:Toluidines / Q000097:blood*
chemical_list: D007306:Insecticides; D014052:Toluidines; C014983:amitraz