title: High quality cord blood banking is feasible with delayed clamping practices. The eight-year experience and current status of the national Swedish Cord Blood Bank.
abstract: The National Swedish Cord Blood Bank (NS-CBB) is altruistic and publicly funded. Herein we describe the status of the bank and the impact of delayed versus early clamping on cell number and volume. Cord Blood Units (CBUs) were collected at two University Hospitals in Sweden. Collected volume and nucleated cell content (TNC) were investigated in 146 consecutive Cord Blood (CB) collections sampled during the first quarter of 2012 and in 162 consecutive CB collections done in the first quarter of 2013, before and after clamping practices were changed from immediate to late (60 s) clamping. NS-CBB now holds close to 5000 units whereof 30 % are from non-Caucasian or mixed origins. Delayed clamping had no major effect on collection efficiency. The volume collected was slightly reduced (mean difference, 8.1 ml; 95 % CI, 1.3-15.0 ml; p = 0.02), while cell recovery was not (p = 0.1). The proportion of CBUs that met initial total TNC banking criteria was 60 % using a TNC threshold of 12.5 × 10(8), and 47 % using a threshold of 15 × 10(8) for the early clamping group and 52 and 37 % in the late clamping group. Following implementation of delayed clamping practices at NS-CBB; close to 40 % of the collections in the late clamping group still met the high TNC banking threshold and were eligible for banking, implicating that that cord blood banking is feasible with delayed clamping practices.
authors.affiliation: Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska University Hospital, University of Gothenburg, Vita stråket 13, 413 45, Gothenburg, Sweden. sofia.frandberg@vgregion.se.
authors.forename: Sofia
authors.identifier: http://orcid.org/0000-0002-5512-5690
authors.initials: S
authors.lastname: Frändberg
authors.affiliation: Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska University Hospital, University of Gothenburg, Vita stråket 13, 413 45, Gothenburg, Sweden.
authors.forename: Berit
authors.initials: B
authors.lastname: Waldner
authors.affiliation: Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska University Hospital, University of Gothenburg, Vita stråket 13, 413 45, Gothenburg, Sweden.
authors.forename: Jan
authors.initials: J
authors.lastname: Konar
authors.affiliation: Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska University Hospital, University of Gothenburg, Vita stråket 13, 413 45, Gothenburg, Sweden.
authors.forename: Lennart
authors.initials: L
authors.lastname: Rydberg
authors.affiliation: Department of Pediatrics at the Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
authors.forename: Anders
authors.initials: A
authors.lastname: Fasth
authors.affiliation: Department of Clinical Chemistry and Transfusion Medicine, Sahlgrenska University Hospital, University of Gothenburg, Vita stråket 13, 413 45, Gothenburg, Sweden.
authors.forename: Jan
authors.initials: J
authors.lastname: Holgersson
mesh_terms: D001771:Blood Banks / Q000592:standards*; D001810:Blood Volume; D002452:Cell Count; D003250:Constriction; D005006:Ethnicity; D005240:Feasibility Studies; D005260:Female; D005312:Fetal Blood / Q000502:physiology*; D006801:Humans; D011247:Pregnancy; D013548:Sweden
keywords: Cord blood; Cord blood bank; Cord clamping; Ethnic minorities; Hematopoietic stem cell transplantation