title: [Study on Rapid Micropropagation in Vitro Technique of Guangfeng Medicinal Yam (Dioscorea opposita) Plantlets].
abstract: OBJECTIVE
  In order to provide methodology reference for virus-free and germplasm conservation of Guangfeng medicinal yam (Dioscorea opposita) plantlets, rapid micropropagation in vitro technique of Guangfeng medicinal yam plantlets was studied.
  
  
  METHODS
  Using the method of plant tissue culture, single factor test and flow-cytometry, the basic procedure of Guangfeng medicinal yam tissue culture was established and the DNA content of Guangfeng medicinal yam plantlets and its potted seedlings was detected.
  
  
  RESULTS
  The best disinfection procedure of stems with a bud of Guangfeng medicinal yam was washed with sterile water for three times after sterilized with 70% alcohol for 20 - 30 s and then washed with sterile water for three times again after sterilized with 0.1% mercuric chloride for 10 - 12 min; The best explants of stems with a bud of Guangfeng medicinal yam was slightly woody and more mature stems witha bud; The best proliferation culture medium of stems with a bud of Guangfeng medicinal yam was MS + 6-BA 2.0 mg/L + NAA 0.1 mg/L; The best rooting culture medium of stems with a bud of Guangfeng medicinal yam was MS + NAA 0.5 mg/L; The best culture method of Guangfeng medicinal yam plantlets was liquid culture; The best transplanting matrix of Guangfeng medicinal yam plantlets was the mixture of paddy clay and fine sand (1: 2) or the mixture of perlite and vermiculite (1: 2); The DNA content between Guangfeng medicinal yam plantlets and its potted seedlings had no significant difference.
  
  
  CONCLUSIONS
  A fast and efficient micropropagation in vitro technological system of stems with a bud of Guangfeng medicinal yam is established, and the flow cytometry detect results also show the genetic stability of Guangfeng medicinal yam plantlets, whose results provide the technical and theoretical basis for the large-scale production of Guangfeng medicinal yam plantlets.
authors.forename: Ming-Hua
authors.initials: MH
authors.lastname: Yin
authors.forename: Zhi-Jian
authors.initials: ZJ
authors.lastname: Xu
authors.forename: Sheng-qin
authors.initials: SQ
authors.lastname: Zhang
authors.forename: Si-Jie
authors.initials: SJ
authors.lastname: Lv
authors.forename: Yan-hong
authors.initials: YH
authors.lastname: Zeng
authors.forename: You-zhang
authors.initials: YZ
authors.lastname: Fu
authors.forename: Sen-rong
authors.initials: SR
authors.lastname: Hong
mesh_terms: D003470:Culture Media; D046508:Culture Techniques; D018744:DNA, Plant / Q000302:isolation & purification; D027742:Dioscorea / Q000254:growth & development*; D010946:Plants, Medicinal / Q000254:growth & development*; D036226:Seedlings / Q000254:growth & development
chemical_list: D003470:Culture Media; D018744:DNA, Plant