title: Massive fish mortality and Cylindrospermopsis raciborskii bloom in Aleksandrovac Lake.
abstract: This paper presents a case study of a massive fish mortality during a Cylindrospermopsis raciborskii bloom in Aleksandrovac Lake, Serbia in mid-December 2012. According to a preliminary investigation of the samples taken on November 6 before the fish mortalities and to extended analyses of samples taken on November 15, no values of significant physicochemical parameters emerged to explain the cause(s) of the fish mortality. No industrial pollutants were apparent at this location, and results excluded the likelihood of bacterial infections. Even after freezing, the dissolved oxygen concentration in the water was sufficient for fish survival. High concentrations of chlorophyll a and phaeophytin occurred in the lake, and phytoplankton bloom samples were lethal in Artemia salina bioassays. A bloom of the cyanobacterium C. raciborskii was recorded during November. Although the A. salina bioassays indicated the presence of toxic compounds in the cyanobacterial cells, the cyanotoxins, microcystins, cylindrospermopsin and saxitoxin were not detected.
authors.affiliation: Department of Biology and Ecology, Faculty of Science, University of Novi Sad, Trg Dositeja Obradovića 2, Novi Sad, 21000, Serbia.|Biochemistry, Faculty of Science and Engineering, Åbo Akademi University, Tykistökatu 6 A, 20520, Turku, Finland.
authors.forename: Zorica
authors.initials: Z
authors.lastname: Svirčev
authors.affiliation: Jaroslav Černi Institute for the Development of Water Resources, Jaroslava Černog 80, Pinosava, Belgrade, 12226, Serbia.
authors.forename: Vesna
authors.initials: V
authors.lastname: Obradović
authors.affiliation: Biological and Environmental Sciences, University of Stirling, Stirling, FK9 4LA, UK.
authors.forename: Geoffrey A
authors.initials: GA
authors.lastname: Codd
authors.affiliation: Jaroslav Černi Institute for the Development of Water Resources, Jaroslava Černog 80, Pinosava, Belgrade, 12226, Serbia.
authors.forename: Prvoslav
authors.initials: P
authors.lastname: Marjanović
authors.affiliation: Biochemistry, Faculty of Science and Engineering, Åbo Akademi University, Tykistökatu 6 A, 20520, Turku, Finland.
authors.forename: Lisa
authors.initials: L
authors.lastname: Spoof
authors.affiliation: Department of Biology and Ecology, Faculty of Science, University of Novi Sad, Trg Dositeja Obradovića 2, Novi Sad, 21000, Serbia. biometatandem@gmail.com.
authors.forename: Damjana
authors.initials: D
authors.lastname: Drobac
authors.affiliation: Department of Biology and Ecology, Faculty of Science, University of Novi Sad, Trg Dositeja Obradovića 2, Novi Sad, 21000, Serbia.
authors.forename: Nada
authors.initials: N
authors.lastname: Tokodi
authors.affiliation: Jaroslav Černi Institute for the Development of Water Resources, Jaroslava Černog 80, Pinosava, Belgrade, 12226, Serbia.
authors.forename: Anđelka
authors.initials: A
authors.lastname: Petković
authors.affiliation: Jaroslav Černi Institute for the Development of Water Resources, Jaroslava Černog 80, Pinosava, Belgrade, 12226, Serbia.
authors.forename: Tanja
authors.initials: T
authors.lastname: Nenin
authors.affiliation: Department of Biology and Ecology, Faculty of Science, University of Novi Sad, Trg Dositeja Obradovića 2, Novi Sad, 21000, Serbia.
authors.forename: Jelica
authors.initials: J
authors.lastname: Simeunović
authors.affiliation: Department of Biology and Ecology, Faculty of Science, University of Novi Sad, Trg Dositeja Obradovića 2, Novi Sad, 21000, Serbia.
authors.forename: Tamara
authors.initials: T
authors.lastname: Važić
authors.affiliation: Department of Biology and Ecology, Faculty of Science, University of Novi Sad, Trg Dositeja Obradovića 2, Novi Sad, 21000, Serbia.|Biochemistry, Faculty of Science and Engineering, Åbo Akademi University, Tykistökatu 6 A, 20520, Turku, Finland.
authors.forename: Jussi
authors.initials: J
authors.lastname: Meriluoto
mesh_terms: D000470:Alkaloids; D000818:Animals; D001427:Bacterial Toxins / Q000633:toxicity; D000087522:Cyanobacteria Toxins; D046891:Cylindrospermopsis / Q000254:growth & development*; D004784:Environmental Monitoring; D005399:Fishes / Q000502:physiology*; D057097:Harmful Algal Bloom; D060106:Lakes / Q000382:microbiology*; D052998:Microcystins; D014498:Uracil / Q000031:analogs & derivatives / Q000633:toxicity; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D000470:Alkaloids; D001427:Bacterial Toxins; D000087522:Cyanobacteria Toxins; D052998:Microcystins; D014874:Water Pollutants, Chemical; C089595:cylindrospermopsin; D014498:Uracil
keywords: Bloom; Cyanobacteria; Cyanotoxins; Serbia