title: OPG and RANKL polymorphisms are associated with alcohol-induced osteonecrosis of the femoral head in the north area of China population in men.
abstract: Alcohol-induced osteonecrosis of the femoral head (ONFH) is an important pathogenesis of nontraumatic ONFH. However, the mechanisms of the pathogenesis are still unknown. Osteoprotegerin (OPG) and receptor activator of nuclear factor-kappa B ligand (RANKL) have been implicated in multiple functions including blocking osteoclast maturation, controlling vascular calcifications, and promoting tumor growth and metastasis. The purpose of this article was to explore the association between OPG and RANKL gene variants and alcohol-induced ONFH. Six hundred seventy male subjects (335 patients and 335 normal individuals) were enrolled in our study. We selected 24 single-nucleotide polymorphisms (SNPs) to evaluate the association between genetic susceptibility variants and alcohol-induced ONFH using the chi-square test and gene model analysis. Overall, the OPG SNPs (rs1032128 and rs11573828) were associated with the strongest increased risk of alcohol-induced ONFH in the recessive model (rs1032128: odds ratio [OR] 1.49, 95% confidence interval [CI] 1.00-2.22, P = 0.04 for G/A; rs11573828: OR 3.32, 95% CI 1.07-10.30, P = 0.03 for T/C). The RANKL SNP rs2200287 was also an increased risk factor (OR 3.65, 95% CI 1.53-8.47, P = 0.003 for T/C) in the recessive model. The rs11573856, rs3134056, and rs1564861 SNPs were considered protective factors for alcohol-induced ONFH. We concluded that OPG and RANKL polymorphisms were associated with the occurrence of alcohol-induced ONFH.
authors.affiliation: Inner Mongolia Medical University, Hohhot, Inner Mongolia Zhengzhou TCM Traumatology Hospital, Zhengzhou, Henan National Engineering Research Center for Miniaturized Detection System, Xi'an, Shanxi The College of Life Sciences Northwest University Department of Orthopedics and Traumatology, The 2nd Affiliated Hospital of Inner Mongolia University, Hohhot, Inner Mongolia, China.
authors.forename: Yizhou
authors.initials: Y
authors.lastname: Li
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Yongchang
authors.initials: Y
authors.lastname: Guo
authors.forename: Quanjian
authors.initials: Q
authors.lastname: Wang
authors.forename: Yongri
authors.initials: Y
authors.lastname: Ouyang
authors.forename: Yuju
authors.initials: Y
authors.lastname: Cao
authors.forename: Tianbo
authors.initials: T
authors.lastname: Jin
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Wang
mesh_terms: D000328:Adult; D000428:Alcohol Drinking / Q000009:adverse effects* / Q000235:genetics / Q000378:metabolism; D002681:China / Q000453:epidemiology; D004247:DNA / Q000235:genetics*; D005260:Female; D005271:Femur Head Necrosis / Q000453:epidemiology / Q000209:etiology / Q000235:genetics*; D005500:Follow-Up Studies; D006801:Humans; D015994:Incidence; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D053244:Osteoprotegerin / Q000235:genetics* / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D053245:RANK Ligand / Q000235:genetics* / Q000378:metabolism; D012189:Retrospective Studies; D055815:Young Adult
chemical_list: D053244:Osteoprotegerin; D053245:RANK Ligand; C505688:TNFSF11 protein, human; D004247:DNA