title: Improving enzymatic hydrolysis of lignocellulosic substrates with pre-hydrolysates by adding cetyltrimethylammonium bromide to neutralize lignosulfonate.
abstract: Two pretreatment methods to overcome recalcitrance of lignocelluloses, sulfite pretreatment (SPORL) and dilute acid (DA), were conducted to pretreat softwood masson pine and hardwood eucalyptus for enzymatic hydrolysis. In the presence of corresponding pre-hydrolysates, adding moderate cetyltrimethylammonium bromide (CTAB) could enhance the enzymatic hydrolysis of the SPORL-pretreated substrates, but had no enhancement for the DA-pretreated substrates. The results showed that sodium lignosulfonate (SL) in pre-hydrolysates and CTAB together had a strong enhancement on the enzymatic hydrolysis of lignocelluloses. The compound of commercial lignosulfonate SXSL and CTAB (SXSL-CTAB) could enhance the substrate enzymatic digestibility (SED) of SPORL-pretreated masson pine from 27.1% to 71.0%, and that of DA-pretreated eucalyptus from 37.6% to 67.9%. The mechanism that CTAB increased the adsorption of SL on lignin to form more effective steric hindrance and reduced the non-productive adsorption of cellulase on lignin by neutralizing the negative charge of SL was proposed.
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Cai
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China; State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, China.
authors.forename: Xueqing
authors.initials: X
authors.lastname: Qiu
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China.
authors.forename: Xuliang
authors.initials: X
authors.lastname: Lin
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China; State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, China. Electronic address: cehmlou@scut.edu.cn.
authors.forename: Hongming
authors.initials: H
authors.lastname: Lou
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China.
authors.forename: Yuxia
authors.initials: Y
authors.lastname: Pang
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China.
authors.forename: Dongjie
authors.initials: D
authors.lastname: Yang
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China.
authors.forename: Siwei
authors.initials: S
authors.lastname: Chen
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China.
authors.forename: Kaifan
authors.initials: K
authors.lastname: Cai
mesh_terms: D000327:Adsorption; D002480:Cellulase / Q000737:chemistry; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry*; D005052:Eucalyptus / Q000737:chemistry; D006868:Hydrolysis; D008031:Lignin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D028223:Pinus / Q000737:chemistry; D013447:Sulfites / Q000737:chemistry; D014934:Wood / Q000737:chemistry*
chemical_list: D002593:Cetrimonium Compounds; D013447:Sulfites; C036909:lignocellulose; C001545:lignosulfuric acid; D008031:Lignin; D002480:Cellulase; D000077286:Cetrimonium
keywords: Cellulase; Cetyltrimethylammonium bromide; Enzymatic hydrolysis; Lignocellulose; Lignosulfonate