title: Optical clearing for luminal organ imaging with ultrahigh-resolution optical coherence tomography.
abstract: The imaging depth of optical coherence tomography (OCT) in highly scattering biological tissues (such as luminal organs) is limited, particularly for OCT operating at shorter wavelength regions (such as around 800 nm). For the first time, the optical clearing effect of the mixture of liquid paraffin and glycerol on luminal organs was explored with ultrahigh-resolution spectral domain OCT at 800 nm. Ex vivo studies were performed on pig esophagus and bronchus, and guinea pig esophagus with different volume ratios of the mixture. We found that the mixture of 40% liquid paraffin had the best optical clearing effect on esophageal tissues with a short effective time of ∼ 10 min, which means the clearing effect occurs about 10 min after the application of the clearing agent. In contrast, no obvious optical clearing effect was identified on bronchus tissues.
authors.affiliation: Johns Hopkins University, Department of Biomedical Engineering, Baltimore, Maryland 21205, United StatesbNankai University, Institute of Modern Optics, Key Laboratory of Optical Information Science and Technology, Ministry of Education, Tianjin 300071, Ch.
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Johns Hopkins University, Department of Biomedical Engineering, Baltimore, Maryland 21205, United States.
authors.forename: Wu
authors.initials: W
authors.lastname: Yuan
authors.affiliation: Johns Hopkins University, Department of Biomedical Engineering, Baltimore, Maryland 21205, United States.
authors.forename: Jessica
authors.initials: J
authors.lastname: Mavadia-Shukla
authors.affiliation: Johns Hopkins University, Department of Biomedical Engineering, Baltimore, Maryland 21205, United States.
authors.forename: Xingde
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D001980:Bronchi / Q000000981:diagnostic imaging* / Q000187:drug effects; D003287:Contrast Media / Q000737:chemistry* / Q000494:pharmacology; D004947:Esophagus / Q000000981:diagnostic imaging* / Q000187:drug effects; D006168:Guinea Pigs; D013552:Swine; D041623:Tomography, Optical Coherence / Q000379:methods*
chemical_list: D003287:Contrast Media