title: Sensitive detection of Porphyromonas gingivalis based on magnetic capture and upconversion fluorescent identification with multifunctional nanospheres.
abstract: A specific and sensitive detection system was designed to detect Porphyromonas gingivalis, a major periodontal pathogen, in mixed bacterial fluids. This new detection system was based on the use of fluorescent and magnetic encoding nanospheres that were conjugated with monoclonal antibodies specific to P. gingivalis, thus enabling rapid detection of the target bacterium. This strategy simplifies the detection process and improves the sensitivity compared with conventional methods, with a detection limit of approximately 10 colony-forming units (CFU) ml(-1) . This new method shows strong anti-interference ability and excellent selectivity and specificity to detect P. gingivalis in mixed solutions.
authors.affiliation: Department of Periodontology, School and Hospital of Stomatology, Tianjin Medical University, Tianjin, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qin
authors.affiliation: School of Life Sciences, Tianjin University, Tianjin Engineering Center of Micro-Nano Biomaterials and Detection-Treatment Technology, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zheng
authors.affiliation: Department of Periodontology, School and Hospital of Stomatology, Tianjin Medical University, Tianjin, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Department of Periodontology, School and Hospital of Stomatology, Tianjin Medical University, Tianjin, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Stomatology, Tianjin Medical University General Hospital, Tianjin, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Bai
authors.affiliation: School of Life Sciences, Tianjin University, Tianjin Engineering Center of Micro-Nano Biomaterials and Detection-Treatment Technology, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Chang
authors.affiliation: School of Life Sciences, Tianjin University, Tianjin Engineering Center of Micro-Nano Biomaterials and Detection-Treatment Technology, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, China.
authors.forename: Hanjie
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Periodontology, School and Hospital of Stomatology, Tianjin Medical University, Tianjin, China.
authors.forename: Yonglan
authors.initials: Y
authors.lastname: Wang
mesh_terms: D004396:Coloring Agents; D008280:Magnetics; D054118:Nanospheres; D010518:Periodontitis; D016966:Porphyromonas gingivalis / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
chemical_list: D004396:Coloring Agents
keywords: Porphyromonas gingivalis; fluorescent encoding nanospheres; magnetic encoding nanospheres; oral microbiota; periodontitis