title: Quantitative inner membrane proteome datasets of the wild-type and the Δmin mutant of Escherichia coli.
abstract: This article presents data that were obtained through measuring the impact of the Min oscillation on membrane proteins in Escherichia coli by quantitative protemoics analysis. We isolated inner membranes from the wild-type and mutant strains to generate proteomics datasets based on NanoLC-nanoESI-MS/MS mass spectrometry using the isobaric tags for relative and absolute quantitation (iTRAQ) method. The datasets included the raw spectral files from four sample replicates and the processed files using Proteome Discoverer that contained a total of 40,072 MS/MS spectra with confident peptide identifier (FDR<0.01) and the peak intensity of the reporter ions. The data was further filtered, which resulted in an inner membrane proteome of unique proteins with quantitation. Proteins of interest, that show significant difference in protein abundance of the mutant membrane, were isolated through statistical filtering. The data is related to "Quantitative proteomics analysis reveals the Min system of Escherichia coli modulates reversible protein association with the inner membrane" (Lee et al., 2016 [1]).
authors.affiliation: Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan.
authors.forename: Suh-Yuen
authors.initials: SY
authors.lastname: Liang
authors.affiliation: Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan.
authors.forename: Shu-Yu
authors.initials: SY
authors.lastname: Lin
authors.affiliation: Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan.
authors.forename: I-Chen
authors.initials: IC
authors.lastname: Chiang
authors.affiliation: Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan; Institute of Biochemical Sciences, National Taiwan University, Taipei 106, Taiwan; Department of Microbiology, College of Medicine, National Taiwan University, Taipei 100, Taiwan.
authors.forename: Yu-Ling
authors.initials: YL
authors.lastname: Shih
keywords: Escherichia coli; Inner membrane protein; Mass spectrometry; The Min system; iTRAQ