title: Angiopoietin-1 facilitates recovery of hematopoiesis in radiated mice.
abstract: Angiopoietin-1 (Ang-1) plays a critical role in the regulation of endothelial cell survival and vascular maturation and stability. However, its role in hematopoiesis is not clear. Here, we determined effect of Ang-1 on the recovery of hematopoiesis in radiated mice. By injecting an Ang-1 plasmid, we found that Ang-1 was preferentially expressed in bone marrow (BM) of femur from radiated mice. This injection resulted in elevated blood counts and serum VEGF level. The blockade in S phase of cell cycle in mouse BM stromal cells following radiation was attenuated by injection of Ang-1 plasmid. In addition, injection of Ang-1 plasmid attenuated the radiation-mediated inhibition of Tie2 expression. Furthermore, through analyzing Notch1 expression, we found that injection of Ang-1 plasmid increased Notch mRNA expression in radiated mice. In conclusion, these findings suggest that Ang-1 facilitates the recovery of hematopoiesis in radiated mice with the involvement of Notch signaling pathway.
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Wenzhou Medical University NanBai Xiang, Ouhai District, Wenzhou 325000, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Sun
authors.affiliation: Department of Orthopaedics, The First Affiliated Hospital of Wenzhou Medical University NanBai Xiang, Ouhai District, Wenzhou 325000, China.
authors.forename: Huaibao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Wenzhou Medical University NanBai Xiang, Ouhai District, Wenzhou 325000, China.
authors.forename: Laixi
authors.initials: L
authors.lastname: Bi
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Wenzhou Medical University NanBai Xiang, Ouhai District, Wenzhou 325000, China.
authors.forename: Yi-Fen
authors.initials: YF
authors.lastname: Shi
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Wenzhou Medical University NanBai Xiang, Ouhai District, Wenzhou 325000, China.
authors.forename: Chongyun
authors.initials: C
authors.lastname: Xing
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Wenzhou Medical University NanBai Xiang, Ouhai District, Wenzhou 325000, China.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Tang
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Wenzhou Medical University NanBai Xiang, Ouhai District, Wenzhou 325000, China.
authors.forename: Songfu
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Wenzhou Medical University NanBai Xiang, Ouhai District, Wenzhou 325000, China.
authors.forename: Kang
authors.initials: K
authors.lastname: Yu
keywords: Angiopoietin-1; Notch; hematopoiesis; signaling pathway