title: Echocardiographic Evaluation of the Acute Cardiovascular Effects of an Endothelin-Like Peptide Extracted from the Venom of Atractaspis irregularis.
abstract: Sarafotoxin-i3 from Atractaspis irregularis is a long sarafotoxin with an extended C terminus extension. Sarafotoxin-b from Atractaspis engaddensis is shorter by four amino acids. These peptides belong to the endothelin-like peptide family with a high sequence homology and similar three-dimensional structure. They act on endothelin receptors situated on the membrane of endothelial and smooth muscle cells. However, SRTX-i 3, despite a high toxicity, has a very low affinity for endothelin receptors compared to SRTX-b. The present work was carried out in order to compare the precise in vivo cardiovascular effect of SRTX-b and SRTX-i3. Male Wistar rats were anesthetized and mechanically ventilated. Doppler echocardiography was performed to measure left and right ventricular functions. The rats were divided into three groups that received intravenous injections of: saline, SRTX-b or SRTX-i3. All measurements were taken at baseline, at 1 min and at 6 min after injection. Both toxins impaired cardiac output. SRTX-b impaired left ventricular function, while SRTX-i3 increased airway pressures and led to acute right ventricular dilatation associated with a decreased tricuspid annulus peak systolic velocity. SRTX-b and SRTX-i3 appear to exert toxic effects via different mechanisms, SRTX-b impairs left ventricular function, while SRTX-i3 increases airway pressures and impairs right ventricular function.
authors.affiliation: Unité de réanimation chirurgicale, Pôle d'Anesthésie Réanimation et médecine d'urgence, CHU Amiens Nord, Place Victor Pauchet, 80054, Amiens cedex, France. mahjoub.yazine@chu-amiens.fr.|Unité INSERM U1088, Amiens, France. mahjoub.yazine@chu-amiens.fr.
authors.forename: Yazine
authors.initials: Y
authors.lastname: Mahjoub
authors.affiliation: Unité de réanimation chirurgicale, Pôle d'Anesthésie Réanimation et médecine d'urgence, CHU Amiens Nord, Place Victor Pauchet, 80054, Amiens cedex, France.|Unité INSERM U1088, Amiens, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Malaquin
authors.affiliation: Unité de réanimation chirurgicale, Pôle d'Anesthésie Réanimation et médecine d'urgence, CHU Amiens Nord, Place Victor Pauchet, 80054, Amiens cedex, France.|Unité INSERM U1088, Amiens, France.
authors.forename: Osama
authors.initials: O
authors.lastname: Abou Arab
authors.affiliation: CEA, iBiTec-S, Service d'Ingénierie Moléculaire des Protéines (SIMOPRO), CEA Saclay, 91191, Gif sur Yvette, France.
authors.forename: Gilles
authors.initials: G
authors.lastname: Mourier
authors.affiliation: Unité de réanimation chirurgicale, Pôle d'Anesthésie Réanimation et médecine d'urgence, CHU Amiens Nord, Place Victor Pauchet, 80054, Amiens cedex, France.|Unité INSERM U1088, Amiens, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Lorne
authors.affiliation: Unité de réanimation chirurgicale, Pôle d'Anesthésie Réanimation et médecine d'urgence, CHU Amiens Nord, Place Victor Pauchet, 80054, Amiens cedex, France.|Unité INSERM U1088, Amiens, France.
authors.forename: Alexis
authors.initials: A
authors.lastname: Salomon
authors.affiliation: Unité INSERM U1088, Amiens, France.
authors.forename: Saïd
authors.initials: S
authors.lastname: Kamel
authors.affiliation: Unité de réanimation chirurgicale, Pôle d'Anesthésie Réanimation et médecine d'urgence, CHU Amiens Nord, Place Victor Pauchet, 80054, Amiens cedex, France.|Unité INSERM U1088, Amiens, France.
authors.forename: Hervé
authors.initials: H
authors.lastname: Dupont
authors.affiliation: CEA, iMETI, Service d'Immuno Virologie (SIV), CEA Fontenay-aux-Roses, 92265, Fontenay-aux-Roses, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Ducancel
mesh_terms: D000818:Animals; D062186:Arterial Pressure / Q000187:drug effects; D002302:Cardiac Output / Q000187:drug effects; D002319:Cardiovascular System / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000503:physiopathology; D015150:Echocardiography, Doppler; D006439:Hemodynamics / Q000187:drug effects*; D008297:Male; D010455:Peptides / Q000633:toxicity*; D011312:Pressure; D017208:Rats, Wistar; D012137:Respiratory System / Q000187:drug effects / Q000503:physiopathology; D013997:Time Factors; D014655:Vascular Resistance / Q000187:drug effects; D016277:Ventricular Function, Left / Q000187:drug effects*; D016278:Ventricular Function, Right / Q000187:drug effects*; D014757:Viper Venoms / Q000633:toxicity*
chemical_list: D010455:Peptides; D014757:Viper Venoms; C000623303:sarafotoxin-i3, Atractaspis irregularis; C057354:sarafotoxins s6
keywords: Cardiovascular; Echocardiography; Endothelins; Right ventricle; Sarafotoxins