title: Solid lipid nanoparticles carrying lipophilic derivatives of doxorubicin: preparation, characterization, and in vitro cytotoxicity studies.
abstract: Doxorubicin (DOXO) lauroyl ester and amide were proposed as lipophilic derivatives and entrapped in SLNs. DOXO derivatives-loaded SLNs were spherical shaped, had 200-300 nm mean diameters and showed 80-94% w/w drug entrapment efficiencies. The effect of DOXO derivatives-loaded SLNs and free DOXO on cell growth was examined by MTT and colony-forming assays on four different tumour cell lines: a pancreatic, CFPAC-1, a lung, A549, and two ovarian, A2780 and A2780res (DOXO-resistant). The results obtained with MTT and colony-forming assay show that although DOXO displayed an inhibition of cell proliferation greater or similar to DOXO lauroyl amide-loaded SLNs on all cell types, the effect induced by DOXO lauroyl ester-loaded SLNs was higher and concentration-dependent, and it was the only one maintained at 10(-5 )mM concentration. Only DOXO lauroyl ester-loaded SLNs were able to induce a 40% inhibitory effect on A2780 res cell line up to 10(-4 )mM concentration.
authors.affiliation: a Dipartimento di Scienza e Tecnologia del Farmaco , University of Turin , Torino , Italy ;
authors.forename: Elena
authors.initials: E
authors.lastname: Peira
authors.affiliation: a Dipartimento di Scienza e Tecnologia del Farmaco , University of Turin , Torino , Italy ;
authors.forename: Daniela
authors.initials: D
authors.lastname: Chirio
authors.affiliation: a Dipartimento di Scienza e Tecnologia del Farmaco , University of Turin , Torino , Italy ;
authors.forename: Luigi
authors.initials: L
authors.lastname: Battaglia
authors.affiliation: a Dipartimento di Scienza e Tecnologia del Farmaco , University of Turin , Torino , Italy ;
authors.forename: Alessandro
authors.initials: A
authors.lastname: Barge
authors.affiliation: a Dipartimento di Scienza e Tecnologia del Farmaco , University of Turin , Torino , Italy ;
authors.forename: Konstantin
authors.initials: K
authors.lastname: Chegaev
authors.affiliation: b Dipartimento di Scienze della Salute , University of Eastern Piedmont Amedeo Avogadro , Novara , Italy.
authors.forename: Casimiro Luca
authors.initials: CL
authors.lastname: Gigliotti
authors.affiliation: a Dipartimento di Scienza e Tecnologia del Farmaco , University of Turin , Torino , Italy ;
authors.forename: Benedetta
authors.initials: B
authors.lastname: Ferrara
authors.affiliation: a Dipartimento di Scienza e Tecnologia del Farmaco , University of Turin , Torino , Italy ;
authors.forename: Chiara
authors.initials: C
authors.lastname: Dianzani
authors.affiliation: a Dipartimento di Scienza e Tecnologia del Farmaco , University of Turin , Torino , Italy ;
authors.forename: Marina
authors.initials: M
authors.lastname: Gallarate
mesh_terms: D045744:Cell Line, Tumor; D003603:Cytotoxins / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D004317:Doxorubicin / Q000031:analogs & derivatives / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D006801:Humans; D008055:Lipids / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D053758:Nanoparticles / Q000737:chemistry*; D009369:Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology
chemical_list: D003603:Cytotoxins; D004337:Drug Carriers; D008055:Lipids; D004317:Doxorubicin
keywords: Coacervation method; SLN; cytotoxicity; doxorubicin