title: A high-fat high-energy diet influences hepatic CYP3A expression and activity in low-birth-weight developing female rats.
abstract: BACKGROUND
  The objective of this study was to investigate the effects of a high-fat, high-energy (HFHE) diet on the hepatic expression of CYP3A in low-birthweight developing female rats.
  
  
  METHODS
  Pregnant rats were divided into nourished and undernourished groups. The offspring of the nourished rats were defined as the normal-birth-weight (NBW) group, and those of undernourished rats were defined as the low-birth-weight (LBW) group. According to their birth weights and diets, the rats were subdivided into the following four groups: NBW-normal diet (NN) group; NBW-HFHE (NH) group; LBW-normal diet (LN) group; and LBW-HFHE (LH) group. Liver samples were isolated on days 3, 7, 14, 21, 28, 56 and 84 after birth.
  
  
  RESULTS
  The CYP3A1 mRNA levels in the LH group on days 3, 56 and 84 were significantly higher than those of the NN group (P<0.05). CYP3A1 expression was significantly higher in the LH group than that in the NH group on days 21, 28 and 84 (P<0.05). CYP3A1 mRNA expression was higher in the LH group than that in the LN group on days 3 and 21 (P<0.05). No zonal CYP3A1 expression pattern was observed in the LH developmental group. The LH group had significantly higher mean activity than the LN group on days 7, 14, 28 and 56.
  
  
  CONCLUSIONS
  Our results indicated that an HFHE diet can result in alterations of CYP3A expression in a developmental LBW rat model.
authors.affiliation: The National Clinical Trial Institute (Ni SQ), Pharmacy Department, Zhejiang University School of Medicine and Zhejiang Key Laboratory for Diagnosis and Therapy of Neonatal Diseases, Hangzhou, China.
authors.forename: Shao-Qing
authors.initials: SQ
authors.lastname: Ni
authors.affiliation: The National Clinical Trial Institute (Ni SQ), Pharmacy Department, Zhejiang University School of Medicine and Zhejiang Key Laboratory for Diagnosis and Therapy of Neonatal Diseases, Hangzhou, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Lou
authors.affiliation: Department of Endocrinology, Zhejiang University School of Medicine and Zhejiang Key Laboratory for Diagnosis and Therapy of Neonatal Diseases, Hangzhou, China.
authors.forename: Xiu-Min
authors.initials: XM
authors.lastname: Wang
authors.affiliation: Lab Center, Zhejiang University School of Medicine and Zhejiang Key Laboratory for Diagnosis and Therapy of Neonatal Diseases, Hangzhou, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Shen
authors.affiliation: The National Clinical Trial Institute (Ni SQ), Pharmacy Department, Zhejiang University School of Medicine and Zhejiang Key Laboratory for Diagnosis and Therapy of Neonatal Diseases, Hangzhou, China.
authors.forename: Jue
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Children's Health and Care Children's Hospital, Zhejiang University School of Medicine and Zhejiang Key Laboratory for Diagnosis and Therapy of Neonatal Diseases, Hangzhou, China.
authors.forename: Zheng-Yan
authors.initials: ZY
authors.lastname: Zhao
authors.affiliation: Department of Pharmaceutical Analysis and Drug Metabolism, College of Pharmaceutical Sciences, Zhejiang University, No. 388 Yu Hang Tang Road, 310058, Hangzhou, China. nsqshc@163.com.
authors.forename: Su
authors.initials: S
authors.lastname: Zeng
mesh_terms: D000818:Animals; D005260:Female; D011247:Pregnancy; D051381:Rats; D000831:Animals, Newborn; D001835:Body Weight; D051544:Cytochrome P-450 CYP3A / Q000235:genetics; D059305:Diet, High-Fat; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D008099:Liver / Q000378:metabolism; D023421:Models, Animal; D017207:Rats, Sprague-Dawley; D012333:RNA, Messenger / Q000032:analysis; D012680:Sensitivity and Specificity
chemical_list: D051544:Cytochrome P-450 CYP3A; D012333:RNA, Messenger
keywords: CYP3A; development; high-energy diet; low birth weight