title: miR156a Mimic Represses the Epithelial-Mesenchymal Transition of Human Nasopharyngeal Cancer Cells by Targeting Junctional Adhesion Molecule A.
abstract: MicroRNAs (miRNAs) have been documented as having an important role in the development of cancer. Broccoli is very popular in large groups of the population and has anticancer properties. Junctional adhesion molecule A (JAMA) is preferentially concentrated at tight junctions and influences cell morphology and migration. Epithelial-mesenchymal transition (EMT) is a developmental program associated with cancer progression and metastasis. In this study we aimed to investigate the role of miRNAs from broccoli in human nasopharyngeal cancer (NPC). We demonstrated that a total of 84 conserved miRNAs and 184 putative novel miRNAs were found in broccoli by sequencing technology. Among these, miR156a was expressed the most. In addition, synthetic miR156a mimic inhibited the EMT of NPC cells in vitro. Furthermore, it was confirmed that JAMA was the target of miR156a mimic as validated by 3' UTR luciferase reporter assays and western blotting. Knockdown of JAMA was consistent with the effects of miR156a mimic on the EMT of NPC, and the up-regulation of JAMA could partially restore EMT repressed by miR156a mimic. In conclusion, these results indicate that the miR156a mimic inhibits the EMT of NPC cells by targeting the 3' UTR of JAMA. These miRNA profiles of broccoli provide a fundamental basis for further research. Moreover, the discovery of miR156a may have clinical implications for the treatment of patients with NPC.
authors.affiliation: Department of Radiation Oncology, Cancer Center of Guangzhou Medical University, Guangzhou, Guangdong Province, People's Republic of China.
authors.forename: Yunhong
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Department of Radiation Oncology, Cancer Center of Guangzhou Medical University, Guangzhou, Guangdong Province, People's Republic of China.
authors.forename: Longmei
authors.initials: L
authors.lastname: Cai
authors.affiliation: Huizhou Municipal Central Hospital, Huizhou, Guangdong Province, People's Republic of China.
authors.forename: Yunming
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Department of Radiation Oncology, Cancer Center of Guangzhou Medical University, Guangzhou, Guangdong Province, People's Republic of China.
authors.forename: Yinuo
authors.initials: Y
authors.lastname: Tu
authors.affiliation: Department of Radiation Oncology, Cancer Center of Guangzhou Medical University, Guangzhou, Guangdong Province, People's Republic of China.
authors.forename: Huizhi
authors.initials: H
authors.lastname: Qiu
authors.affiliation: Department of Radiation Oncology, Cancer Center of Guangzhou Medical University, Guangzhou, Guangdong Province, People's Republic of China.
authors.forename: Guofeng
authors.initials: G
authors.lastname: Xie
authors.affiliation: Department of Radiation Oncology, Cancer Center of Guangzhou Medical University, Guangzhou, Guangdong Province, People's Republic of China.
authors.forename: Donglan
authors.initials: D
authors.lastname: Huang
authors.affiliation: Department of Radiation Oncology, Cancer Center of Guangzhou Medical University, Guangzhou, Guangdong Province, People's Republic of China.
authors.forename: Ronghui
authors.initials: R
authors.lastname: Zheng
authors.affiliation: Department of Radiation Oncology, Cancer Center of Guangzhou Medical University, Guangzhou, Guangdong Province, People's Republic of China.
authors.forename: Weijun
authors.initials: W
authors.lastname: Zhang
mesh_terms: D020413:3' Untranslated Regions; D001665:Binding Sites; D001937:Brassica / Q000235:genetics; D045744:Cell Line, Tumor; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D062745:Junctional Adhesion Molecule A / Q000235:genetics* / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics*; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D010641:Phenotype; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D034622:RNA Interference; D018749:RNA, Plant
chemical_list: D020413:3' Untranslated Regions; D062745:Junctional Adhesion Molecule A; D035683:MicroRNAs; D018749:RNA, Plant; D051057:Proto-Oncogene Proteins c-akt