title: Expanding the tools for identifying mononuclear phagocyte subsets in swine: Reagents to porcine CD11c and XCR1.
abstract: Pig is a domestic species of major importance in the agro-economy and in biomedical research. Mononuclear phagocytes (MNP) are organized in subsets with specialized roles in the orchestration of the immune response and new tools are awaited to improve MNP subset identification in the pig. We cloned pig CD11c cDNA and generated a monoclonal antibody to pig CD11c which showed a pattern of expression by blood and skin MNP subsets similar to humans. We also developed a porcine XCL1-mCherry dimer which specifically reacted with the XCR1-expressing dendritic cell subset of the type 1 lineage in blood and skin. These original reagents will allow the efficient identification of pig MNP subsets to study their role in physiological and pathological processes and also to target these cells in novel intervention and vaccine strategies for veterinary applications and preclinical evaluations.
authors.affiliation: VIM-INRA-Université Paris-Saclay, Domaine de Vilvert, 78350, Jouy-en-Josas, France.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Deloizy
authors.affiliation: VIM-INRA-Université Paris-Saclay, Domaine de Vilvert, 78350, Jouy-en-Josas, France.
authors.forename: Edwige
authors.initials: E
authors.lastname: Bouguyon
authors.affiliation: K.G. Jebsen Center for Research on Influenza Vaccines, University of Oslo and Oslo University Hospital, 0027, Oslo, Norway.
authors.forename: Even
authors.initials: E
authors.lastname: Fossum
authors.affiliation: Institute of Microbiology of the Czech Academy of Sciences, v.v.i., 142 20, Prague, Czech Republic.
authors.forename: Peter
authors.initials: P
authors.lastname: Sebo
authors.affiliation: Institute of Microbiology of the Czech Academy of Sciences, v.v.i., 142 20, Prague, Czech Republic.
authors.forename: Radim
authors.initials: R
authors.lastname: Osicka
authors.affiliation: MI-mAbs, Parc Scientifique et Technologique de Luminy, Case 906, F13288, Marseille Cedex 9, France.
authors.forename: Angélique
authors.initials: A
authors.lastname: Bole
authors.affiliation: MI-mAbs, Parc Scientifique et Technologique de Luminy, Case 906, F13288, Marseille Cedex 9, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Pierres
authors.affiliation: VIM-INRA-Université Paris-Saclay, Domaine de Vilvert, 78350, Jouy-en-Josas, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Biacchesi
authors.affiliation: Centre d'Immunologie de Marseille-Luminy, Aix Marseille Université UM2, Inserm, U1104, CNRS UMR7280, 13288, Marseille, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Dalod
authors.affiliation: K.G. Jebsen Center for Research on Influenza Vaccines, University of Oslo and Oslo University Hospital, 0027, Oslo, Norway; Center for Immune Regulation, Institute of Immunology, University of Oslo and Oslo University Hospital Rikshospitalet, 0424, Oslo, Norway.
authors.forename: Bjarne
authors.initials: B
authors.lastname: Bogen
authors.affiliation: VIM-INRA-Université Paris-Saclay, Domaine de Vilvert, 78350, Jouy-en-Josas, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Bertho
authors.affiliation: VIM-INRA-Université Paris-Saclay, Domaine de Vilvert, 78350, Jouy-en-Josas, France. Electronic address: isabelle.schwartz@jouy.inra.fr.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Schwartz-Cornil
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D039521:CD11c Antigen / Q000276:immunology / Q000378:metabolism*; D003001:Cloning, Molecular; D003713:Dendritic Cells / Q000502:physiology*; D004195:Disease Models, Animal; D006801:Humans; D007159:Immunologic Tests / Q000379:methods; D012157:Mononuclear Phagocyte System; D010586:Phagocytes / Q000276:immunology*; D043562:Receptors, G-Protein-Coupled / Q000276:immunology / Q000378:metabolism*; D012867:Skin / Q000378:metabolism*; D013552:Swine / Q000276:immunology*; D014730:Veterinary Medicine
chemical_list: D000911:Antibodies, Monoclonal; D039521:CD11c Antigen; D043562:Receptors, G-Protein-Coupled
keywords: CD11c; Dendritic cells; Mononuclear phagocytes; Pig model; XCR1