title: Elevated level of pro inflammatory cytokine and chemokine expression in chicken bone marrow and monocyte derived dendritic cells following LPS induced maturation.
abstract: The study was designed to characterize and compare chicken bone marrow and peripheral blood monocyte derived dendritic cells (chBM-DC and chMoDC) and to evaluate inflammatory cytokine and chemokine alterations in response upon LPS stimulation. Typical morphology was observed in DCs from 48h of culture using recombinant chicken GM-CSF and IL-4. Maturation of DCs with LPS (1μg/ml) showed significant up regulation of mRNA of surface markers (CD40, CD80, CD83, CD86, MHC-II and DC-LAMP (CD208)), pro-inflammatory cytokines (IL-1β, IL-6, TNF-α (LITAF)), iNOS, chemokine CXCli2 and TLRs4 and 15. Basal level of TLR1 mRNA expression was higher followed by TLR15 in both DCs irrespective of their origin. Expression of iNOS and CXCLi2 mRNA in mature DCs of both origins were higher than other surface molecules and cytokines studied. Hence, its level of expression can also be used as an additional maturation marker for LPS induced chicken dendritic cell maturation along with CD83 and CD40. LPS matured DCs of both origins upregulated IL-12 and IFN-γ. Based on CD40 and CD83 mRNA expression, it was observed that LPS induced the maturation in both DCs, but chMoDCs responded better in expression of surface markers and inflammatory mediator genes.
authors.affiliation: ICAR- National Institute of High Security Animal Diseases, Anand Nagar, Bhopal, Madhya Pradesh 462 022, India. Electronic address: kalai_82vetmic@yahoo.co.in.
authors.forename: Semmannan
authors.initials: S
authors.lastname: Kalaiyarasu
authors.affiliation: ICAR- National Institute of High Security Animal Diseases, Anand Nagar, Bhopal, Madhya Pradesh 462 022, India.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Bhatia
authors.affiliation: ICAR- National Institute of High Security Animal Diseases, Anand Nagar, Bhopal, Madhya Pradesh 462 022, India.
authors.forename: Niranjan
authors.initials: N
authors.lastname: Mishra
authors.affiliation: ICAR- National Institute of High Security Animal Diseases, Anand Nagar, Bhopal, Madhya Pradesh 462 022, India.
authors.forename: Richa
authors.initials: R
authors.lastname: Sood
authors.affiliation: ICAR- National Institute of High Security Animal Diseases, Anand Nagar, Bhopal, Madhya Pradesh 462 022, India.
authors.forename: Manoj
authors.initials: M
authors.lastname: Kumar
authors.affiliation: ICAR- National Institute of High Security Animal Diseases, Anand Nagar, Bhopal, Madhya Pradesh 462 022, India.
authors.forename: D
authors.initials: D
authors.lastname: SenthilKumar
authors.affiliation: ICAR- National Institute of High Security Animal Diseases, Anand Nagar, Bhopal, Madhya Pradesh 462 022, India.
authors.forename: Sushant
authors.initials: S
authors.lastname: Bhat
authors.affiliation: ICAR- National Institute of High Security Animal Diseases, Anand Nagar, Bhopal, Madhya Pradesh 462 022, India.
authors.forename: M
authors.initials: M
authors.lastname: Dass Prakash
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D001853:Bone Marrow / Q000187:drug effects / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D054426:Chemokine CXCL2 / Q000378:metabolism; D002645:Chickens / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism*; D003713:Dendritic Cells / Q000187:drug effects / Q000378:metabolism*; D007249:Inflammation / Q000378:metabolism*; D018664:Interleukin-12 / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; D054426:Chemokine CXCL2; D016207:Cytokines; D053583:Interleukin-1beta; D015850:Interleukin-6; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha; D018664:Interleukin-12; D015847:Interleukin-4; D052247:Nitric Oxide Synthase Type II
keywords: CXCLi2; Cytokine; Dendritic cell; Lipopolysaccharide; Surface marker; Toll like receptors; iNOS