title: Antibacterial Activity of Silver Doped Titanate Nanowires on Ti Implants.
abstract: A nanostructured film composed of one-dimensional titanate nanowires (TNWs) was employed as a carrier of Ag nanoparticles and chitosan (CS) to improve the surface antibacterial activity and biocompatibility of titanium implants. A TNWs film was produced on a Ti substrate by an alkali hydrothermal reaction and subsequently doped by Ag nanoparticles through an ultraviolet light chemical reduction. The CS nanofilm was deposited on the Ag nanoparticles through a spin-assisted layer by layer assembly method. The results disclosed that Ag nanoparticles were successfully carried by TNWs and homogeneously distributed on the entire surface. Moreover, a CS nanofilm was also successfully deposited on the Ag nanoparticles. Antibacterial tests showed that the samples modified with a higher initial concentration of AgNO3 solution exhibited better antibacterial activity, and that a CS nanofilm could further improve the antibacterial activity of the TNWs. Cell viability and ALP tests revealed that the release of Ag(+) was detrimental for the growth, proliferation, and differentiation of MC3T3, and that CS could lower the negative effects of Ag gradually as the incubation time increased.
authors.affiliation: Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry-of-Education Key Laboratory for the Green Preparation and Application of Functional Materials, Hubei Key Laboratory of Polymer Materials, School of Materials Science & Engineering, Hubei University , Wuhan, 430062, China.
authors.forename: Ziqiang
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry-of-Education Key Laboratory for the Green Preparation and Application of Functional Materials, Hubei Key Laboratory of Polymer Materials, School of Materials Science & Engineering, Hubei University , Wuhan, 430062, China.
authors.forename: Man
authors.initials: M
authors.lastname: Li
authors.affiliation: Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry-of-Education Key Laboratory for the Green Preparation and Application of Functional Materials, Hubei Key Laboratory of Polymer Materials, School of Materials Science & Engineering, Hubei University , Wuhan, 430062, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.affiliation: Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry-of-Education Key Laboratory for the Green Preparation and Application of Functional Materials, Hubei Key Laboratory of Polymer Materials, School of Materials Science & Engineering, Hubei University , Wuhan, 430062, China.
authors.forename: Xiangmei
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Mechanical Behavior of Materials, School of Materials Science and Engineering, Xi'an Jiaotong University , Xi'an, Shaanxi, 710049, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Ma
authors.affiliation: Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry-of-Education Key Laboratory for the Green Preparation and Application of Functional Materials, Hubei Key Laboratory of Polymer Materials, School of Materials Science & Engineering, Hubei University , Wuhan, 430062, China.
authors.forename: Shuilin
authors.initials: S
authors.lastname: Wu
authors.affiliation: Division of Spine Surgery, Department of Orthopaedics & Traumatology, Li KaShing Faculty of Medicine, The University of Hong Kong , Hong Kong, 999077, China.
authors.forename: K W K
authors.initials: KW
authors.lastname: Yeung
authors.affiliation: State Key Laboratory for Mechanical Behavior of Materials, School of Materials Science and Engineering, Xi'an Jiaotong University , Xi'an, Shaanxi, 710049, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Han
authors.affiliation: Department of Physics & Materials Science, City University of Hong Kong , Tat Chee Avenue, Kowloon, Hong Kong, 999077, China.
authors.forename: Paul K
authors.initials: PK
authors.lastname: Chu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry; D051379:Mice; D053770:Nanowires / Q000737:chemistry* / Q000633:toxicity; D019736:Prostheses and Implants / Q000382:microbiology*; D012834:Silver / Q000494:pharmacology*; D014025:Titanium / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D012834:Silver; D048271:Chitosan; D014025:Titanium
keywords: Ag nanoparticle; antibacterial; implant; nanostructured film; nanowire; titanium