title: Uprolides N, O and P from the Panamanian Octocoral Eunicea succinea.
abstract: Three new diterpenes, uprolide N (1), uprolide O (2), uprolide P (3) and a known one, dolabellane (4), were isolated from the CH₂Cl₂-MeOH extract of the gorgonian octocoral Eunicea succinea, collected from Bocas del Toro, on the Caribbean coast of Panama. Their structures were determined using spectroscopic analyses, including 1D and 2D NMR and high-resolution mass spectrometry (HRMS) together with molecular modeling studies. Compounds 1-3 displayed anti-inflammatory properties by inhibiting production of Tumor Necrosis Factor (TNF) and Interleukin (IL)-6 induced by lipopolysaccharide (LPS) in murine macrophages.
authors.affiliation: Center for Biodiversity and Drug Discovery, Institute for Scientific Research and Technology Services (INDICASAT), Clayton, City of Knowledge 0843-01103, Panama. dtorres@indicasat.org.pa.|Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur 522510, India. dtorres@indicasat.org.pa.
authors.forename: Daniel
authors.initials: D
authors.lastname: Torres-Mendoza
authors.affiliation: Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur 522510, India. yisettgonzalez@gmail.com.|Center for Molecular and Cellular Biology of Diseases, Institute for Scientific Research and Technology Services (INDICASAT), Clayton, City of Knowledge 0843-01103, Panama. yisettgonzalez@gmail.com.
authors.forename: Yisett
authors.initials: Y
authors.lastname: González
authors.affiliation: Center for Biodiversity and Drug Discovery, Institute for Scientific Research and Technology Services (INDICASAT), Clayton, City of Knowledge 0843-01103, Panama. gomezjosefelix@gmail.com.
authors.forename: José Félix
authors.initials: JF
authors.lastname: Gómez-Reyes
authors.affiliation: Smithsonian Tropical Research Institute, Balboa, Ancon 0843-03092, Panama. guzmanh@si.edu.
authors.forename: Héctor M
authors.initials: HM
authors.lastname: Guzmán
authors.affiliation: Department of Pharmaceutical Sciences, Faculty of Pharmacy, University of Salamanca, Ave. Campo Charro, s/n, Salamanca 37007, Spain. lopez@usal.es.
authors.forename: José Luis
authors.initials: JL
authors.lastname: López-Perez
authors.affiliation: Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography and The Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California at San Diego, La Jolla, CA 92037, USA. wgerwick@ucsd.edu.
authors.forename: William H
authors.initials: WH
authors.lastname: Gerwick
authors.affiliation: Center for Molecular and Cellular Biology of Diseases, Institute for Scientific Research and Technology Services (INDICASAT), Clayton, City of Knowledge 0843-01103, Panama. pllanes@indicasat.org.pa.
authors.forename: Patricia L
authors.initials: PL
authors.lastname: Fernandez
authors.affiliation: Center for Biodiversity and Drug Discovery, Institute for Scientific Research and Technology Services (INDICASAT), Clayton, City of Knowledge 0843-01103, Panama. mgutierrez@indicasat.org.pa.
authors.forename: Marcelino
authors.initials: M
authors.lastname: Gutiérrez
mesh_terms: D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D004224:Diterpenes / Q000008:administration & dosage / Q000737:chemistry* / Q000302:isolation & purification; D005786:Gene Expression Regulation / Q000187:drug effects; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy* / Q000235:genetics; D015850:Interleukin-6 / Q000096:biosynthesis / Q000235:genetics; D008264:Macrophages / Q000187:drug effects*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008958:Models, Molecular; D010176:Panama; D010936:Plant Extracts / Q000737:chemistry; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics
chemical_list: D004224:Diterpenes; D015850:Interleukin-6; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; C115566:dolabellane
keywords: Eunicea succinea; anti-inflammatory; cembranolide diterpenes; uprolides