title: [Effects of RhoA gene silencing by RNA interference on invasion of tongue carcinoma].
abstract: OBJECTIVE
  To study the effects of RhoA down-regulation by RNA interference on the invasion of tongue carcinoma Tca8113 and SCC-4.
  
  
  METHODS
  Determination of the human RhoA sequence as well as the design and constructionof a short specific small interfering RNAs (siRNA) were performed. The siRNA of RhoA gene was transfected into humantongue squamous cell carcinoma Tca8113 and SCC-4 cells line by Lipofectamine 2000. Quantitative real-time polymerasechain reaction was used to examine the mRNA expressionlevels of RhoA. Protein expressions of mRNA, galectin-3,and matrix metalloproteinase (MMP)-9 were evaluated byWestern blot. Transwell invasion assay was performed toassess the invasion ability of tongue carcinoma.
  
  
  RESULTS
  RhoA expressions in Tca8113 and SCC-4 cells were reducedsignificantly after transfection of RhoA-siRNA. Protein levels f galectin-3 and MVP-9 were also down-regulated significantly. Invasion ability was inhibited as well.
  
  
  CONCLUSIONS
  RhoA-siRNA can effectively inhibit RhoA expression in Tca8113 and SCC-4 cells. The invasion ability of tongue carcinoma cells decreased with down-regulation of the protein expressions of galectin-3 and MMP-9, indicating that RhoA-siRNA can inhibit invasion of tongue carcinoma. Results show that RhoA may play an important role in the processes of invasion and metastasis of tongue carcinoma.
authors.forename: Zhenggang
authors.initials: Z
authors.lastname: Chen
authors.forename: Yong-ping
authors.initials: YP
authors.lastname: Tang
authors.forename: Lei
authors.initials: L
authors.lastname: Tong
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Qimin
authors.initials: Q
authors.lastname: Wang
authors.forename: Jinhong
authors.initials: J
authors.lastname: Han
authors.forename: Zongxuan
authors.initials: Z
authors.lastname: He
authors.forename: Yixiang
authors.initials: Y
authors.lastname: Liao
authors.forename: Bing
authors.initials: B
authors.lastname: Fan
authors.forename: Rong-hai
authors.initials: RH
authors.lastname: Zou
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Sun
authors.forename: Guoxin
authors.initials: G
authors.lastname: Yan
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D015536:Down-Regulation; D037502:Galectin 3 / Q000378:metabolism; D020868:Gene Silencing; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D014062:Tongue Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D014162:Transfection
chemical_list: D037502:Galectin 3; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D020780:Matrix Metalloproteinase 9