title: Hypoxia-induced microRNA-301b regulates apoptosis by targeting Bim in lung cancer.
abstract: OBJECTIVE
  Worldwide, lung cancer accounts for the majority of cancer-related deaths. Aberrant expression of miRNAs has increasingly been reported to be associated with tumour progression. This study aimed to explore the role of miR-301b in regulating apoptosis in lung cancer.
  
  
  METHODS
  Expression of miR-301b was assessed by real-time PCR in cell lines, human patient tissues and cells treated under hypoxia and DMOG. Scramble siRNA, miR-301b inhibitor and miR-301b mimics were transfected into lung cancer cells to determine their effects on apoptosis. Additionally, a mouse xenograft model was used to explore functions of miR-301b on apoptosis, in vivo. Finally, relationships between Bim and miR-301b levels were explored by luciferase reporter assay and Western blotting.
  
  
  RESULTS
  We found that miR-301b was highly expressed in lung cancer tissues and cell lines. Expression of miR-301b was induced by hypoxia, and miR-301b suppressed expression of Bim by targeting its 3'UTR. Functionally, ectopic expression of miR-301b enhanced cell population growth, reduced apoptosis and reduced sensitivity of cells to chemotherapy. In the xenograft model, overexpression of miR-301b promoted tumour growth. Additionally, miR-301b and Bim expression were inversely correlated in clinical lung cancer samples.
  
  
  CONCLUSIONS
  This study provides new insights into the function of miRNA-301b in lung cancer and suggests that miRNA-301b could be a potential molecular target for chemotherapy.
authors.affiliation: Department of Oncology, NanfangHospital Southern Medical University, Guangzhou, China.|Department of Thoracic Surgery, The Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University, Guangzhou, China.
authors.forename: Duoguang
authors.initials: D
authors.lastname: Wu
authors.affiliation: Department of Oncology, NanfangHospital Southern Medical University, Guangzhou, China.|Department of Thoracic Surgery, The Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University, Guangzhou, China.
authors.forename: Baishen
authors.initials: B
authors.lastname: Chen
authors.affiliation: Department of Oncology, NanfangHospital Southern Medical University, Guangzhou, China.|Department of Thoracic Surgery, The Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University, Guangzhou, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Cui
authors.affiliation: Department of Oncology, NanfangHospital Southern Medical University, Guangzhou, China.|Department of Thoracic Surgery, The Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University, Guangzhou, China.
authors.forename: Xiaotian
authors.initials: X
authors.lastname: He
authors.affiliation: Department of Oncology, NanfangHospital Southern Medical University, Guangzhou, China.|Department of Thoracic Surgery, The Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University, Guangzhou, China.
authors.forename: Wenjian
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Oncology, NanfangHospital Southern Medical University, Guangzhou, China.|Department of Thoracic Surgery, The Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University, Guangzhou, China.
authors.forename: Minghui
authors.initials: M
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis; D000072224:Bcl-2-Like Protein 11 / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D000860:Hypoxia / Q000150:complications / Q000235:genetics* / Q000473:pathology; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008175:Lung Neoplasms / Q000150:complications / Q000235:genetics* / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D015854:Up-Regulation
chemical_list: D000072224:Bcl-2-Like Protein 11; C550735:MIRN301A microRNA, human; D035683:MicroRNAs