title: Gold nanoparticles and polyethylene glycols functionalized conducting polyaniline nanowires for ultrasensitive and low fouling immunosensing of alpha-fetoprotein.
abstract: An ultrasensitive biosensor for alpha-fetoprotein was developed based on electrochemically synthesized polyaniline (PANI) nanowires, which were functionalized with gold nanoparticles (AuNPs) and polyethylene glycols (PEG). The prepared PEG/AuNPs/PANI composite, combining the electrical conductivity of the AuNPs/PANI with the robust antifouling ability of PEG, offered an ideal substrate for the development of low fouling electrochemical biosensors. Alpha-fetoprotein (AFP), a well-known hepatocellular carcinoma biomarker, was used as a model analyte, and its antibody was immobilized on the PEG/AuNPs/PANI for the construction of the AFP immunosensor. Using the redox current of PANI as the sensing signal, in addition to the good biocompatibility of PEG/AuNPs and the anti-biofouling property of PEG, the developed immunosensor showed improved biosensing performances, such as wide linear range and ultralow detection limit (0.007pgmL(-1)). More importantly, it is label-free, reagentless and low fouling, making it capable of assaying AFP in real serum samples without suffering from significant interference or biofouling.
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
authors.forename: Ni
authors.initials: N
authors.lastname: Hui
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
authors.forename: Xiaotian
authors.initials: X
authors.lastname: Sun
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
authors.forename: Zhiling
authors.initials: Z
authors.lastname: Song
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
authors.forename: Shuyan
authors.initials: S
authors.lastname: Niu
authors.affiliation: Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China. Electronic address: xiliangluo@qust.edu.cn.
authors.forename: Xiliang
authors.initials: X
authors.lastname: Luo
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology; D001774:Blood Chemical Analysis / Q000295:instrumentation*; D003217:Conductometry / Q000295:instrumentation*; D004553:Electric Conductivity; D004866:Equipment Contamination / Q000517:prevention & control; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006046:Gold / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation*; D053768:Metal Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D053770:Nanowires / Q000737:chemistry* / Q000648:ultrastructure; D011092:Polyethylene Glycols / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013499:Surface Properties; D000509:alpha-Fetoproteins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology
chemical_list: D000814:Aniline Compounds; D000911:Antibodies, Monoclonal; D000509:alpha-Fetoproteins; C416807:polyaniline; D011092:Polyethylene Glycols; D006046:Gold
keywords: Alpha fetoprotein; Gold nanoparticles; Immunosensor; Polyaniline nanowires; Polyethylene glycol