title: Effect of topical anti-Streptococcus mutans IgY gel on quantity of S. mutans on rats' tooth surface.
abstract: This study aims to evaluate the effect of anti-Streptococcus mutans IgY gel on quantity of S. mutans on rats' tooth surface. Sprague Dawley rats were exposed intra-orally with S. mutans Xc and were fed a caries-inducing diet 2000. The 24 rats were divided into four groups: group A had their teeth coated with IgY gel; group B received sterilized water as a control; group C had their teeth coated with IgY gel starting on the 29(th) day; and group D had their teeth coated with a gel without IgY. Plaque samples were swabbed from the anterior teeth for S. mutans colony quantification, and saliva was collected to measure immunoreactivity by enzyme-linked immunosorbent assay. The results indicated that the quantity of S. mutans in rats treated with IgY gel showed significant difference compared with the controls. After coating with IgY anti-S. mutans gel, the mean immunoreactivity in rat saliva was higher than that of the no treatment group. In conclusion, topical application with anti-S. mutans IgY gel reduced the quantity of S. mutans on the tooth surface.
authors.affiliation: Department of Oral Biology, Oral Sciences Research Center, Faculty of Dentistry, Universitas Indonesia , Indonesia.
authors.forename: Endang W
authors.initials: EW
authors.lastname: Bachtiar
authors.affiliation: Palapa Dentists, Dental Center , Jakarta, Indonesia.
authors.forename: Anggraeni
authors.initials: A
authors.lastname: Afdhal
authors.affiliation: Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia , Indonesia.
authors.forename: Ratna
authors.initials: R
authors.lastname: Meidyawati
authors.affiliation: Department of Infectious Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, Bogor Agricultural University , Indonesia.
authors.forename: Retno D
authors.initials: RD
authors.lastname: Soejoedono
authors.affiliation: Department of Pharmacy, Faculty of Medicine, Universitas Indonesia , Indonesia.
authors.forename: Erni
authors.initials: E
authors.lastname: Poerwaningsih
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000008:administration & dosage*; D003731:Dental Caries / Q000188:drug therapy* / Q000382:microbiology; D005260:Female; D005782:Gels / Q000008:administration & dosage; D007136:Immunoglobulins / Q000008:administration & dosage*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013295:Streptococcus mutans / Q000187:drug effects* / Q000254:growth & development* / Q000276:immunology; D014070:Tooth / Q000382:microbiology
chemical_list: D000907:Antibodies, Bacterial; D005782:Gels; C016512:IgY; D007136:Immunoglobulins
keywords: IgY gel; S. mutans; caries