title: Chromosome aberrations [dup(1q)] in endometrial cancer: Gene analysis of 54 surgical specimens in Turkey.
abstract: OBJECTIVE
  We aimed to evaluate the frequency of chromosomal aberrations and mutations in the k-ras or Her-2/neu genes in surgical specimens of endometrial carcinoma and their association with clinicopathological findings.
  
  
  METHODS
  Fifty-four patients who were treated for endometrial cancer between April 2010 and May 2011 at the Kocaeli University Obstetrics and Gynecology Department, Kocaeli, Turkey were enrolled in a prospective study. Clinical and histopathological findings were recorded. Genetic analysis, which included the detection of chromosomal deletions and duplications, as well as k-ras and Her-2/neu mutations, was performed on endometrial samples from surgical specimens.
  
  
  RESULTS
  In 70% of cases, tumor size was >2 cm or covered the entire uterine cavity, affecting mostly corpus (76%) and invading less than half of the myometrium (80%). Forty-six cases (86%) had endometrioid-type carcinoma, and early stage (Stage I, 65%) and higher grade (Grade II-III, 66%) tumors were predominant. Lymph node and lymphovascular involvement was positive in 11% and 28% of the patients, respectively. Chromosomal aberrations (deletion or duplication) and Her-2/neu and k-ras mutations were encountered in 44%, 15%, and 13% of surgical specimens, respectively. The most common chromosomal aberration was dup(1q) (n = 16). Oncogenic mutations in Her-2/neu or k-ras had no association with the severity of endometrial cancer, but the presence of chromosomal aberrations, as a whole or dup(1q) alone, were associated with higher tumor size, deeper myometrial invasion, advanced stage or grade, lymphovascular invasion, and lymph node involvement (p < 0.05 for all).
  
  
  CONCLUSIONS
  Chromosomal aberrations, particularly dup(1q), are related to advanced disease in endometrial cancer. Genetic analysis of cancer tissues may provide important insights in determining disease prognosis.
authors.affiliation: Department of Obstetrics and Gynecology, Sakarya University School of Medicine, Sakarya Research and Education Hospital, Sakarya, Turkey.
authors.forename: Erman
authors.initials: E
authors.lastname: Sever
authors.affiliation: Department of Obstetrics and Gynecology, Kocaeli University Faculty of Medicine, Kocaeli, Turkey.
authors.forename: Emek
authors.initials: E
authors.lastname: Döğer
authors.affiliation: Department of Obstetrics and Gynecology, Sakarya University School of Medicine, Sakarya Research and Education Hospital, Sakarya, Turkey. Electronic address: doktor1977@hotmail.com.
authors.forename: Serkan
authors.initials: S
authors.lastname: Kumbasar
authors.affiliation: Faculty of Health Sciences, Aydın University, İstanbul, Turkey.
authors.forename: Bulat Aytek
authors.initials: BA
authors.lastname: Şık
authors.affiliation: Department of Obstetrics and Gynecology, Sakarya University School of Medicine, Sakarya Research and Education Hospital, Sakarya, Turkey.
authors.forename: Muzaffer
authors.initials: M
authors.lastname: Temur
authors.affiliation: Department of Radiation Oncology, Onkomer Oncology and Medical Center, İzmir, Turkey.
authors.forename: Hasan Taylan
authors.initials: HT
authors.lastname: Yılmaz
authors.affiliation: Department of Obstetrics and Gynecology, Manisa Merkezefendi Hospital, Manisa, Turkey.
authors.forename: Özgür
authors.initials: Ö
authors.lastname: Yılmaz
authors.affiliation: Department of Obstetrics and Gynecology, Aydın Private Ege Liva Hospital, Aydın, Turkey.
authors.forename: Pelin Ozun
authors.initials: PO
authors.lastname: Özbay
authors.affiliation: Department of Obstetrics and Gynecology, Kocaeli University Faculty of Medicine, Kocaeli, Turkey.
authors.forename: İzzet
authors.initials: İ
authors.lastname: Yücesoy
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018269:Carcinoma, Endometrioid / Q000235:genetics* / Q000556:secondary* / Q000601:surgery; D002872:Chromosome Deletion; D058674:Chromosome Duplication; D002878:Chromosomes, Human, Pair 1; D016889:Endometrial Neoplasms / Q000235:genetics* / Q000473:pathology* / Q000601:surgery; D005260:Female; D018734:Genes, erbB-2; D006801:Humans; D008207:Lymphatic Metastasis; D008875:Middle Aged; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011446:Prospective Studies; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics*; D047368:Tumor Burden; D014421:Turkey
chemical_list: C117307:KRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)
keywords: Her-2/neu; chromosomal abnormalities; endometrial cancer; k-ras