title: Inhibition of glycogen synthase kinase-3 (GSK3) promotes the neural differentiation of full-term amniotic fluid-derived stem cells towards neural progenitor cells.
abstract: The amniotic fluid has a heterogeneous population of cells. Some human amniotic fluid-derived stem (hAFS) cells have been shown to harbor the potential to differentiate into neural cells. However, the neural differentiation efficiency of hAFS cells remains low. In this study, we isolated CD117-positive hAFS cells from amniotic fluid and then examined the pluripotency of these cells through the formation of embryoid bodies (EBs). Additionally, we induced the neural differentiation of these cells using neuroectodermal medium. This study revealed that the GSK3-beta inhibitor SB216763 was able to stimulate the proliferation of CD117-positive hAFS cells without influencing their undifferentiated state. Moreover, SB216763 can efficiently promote the neural differentiation of CD117-positive hAFS cells towards neural progenitor cells in the presence of DMEM/F12 and N2 supplement. These findings provide an easy and low-cost method to maintain the proliferation of hAFS cells, as well as induce an efficacious generation of neural progenitor cells from hAFS cells. Such induction of the neural commitment of hAFS cells may provide an option for the treatment of neurodegenerative diseases by hAFS cells-based therapies.
authors.affiliation: Stem Cell Research Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China. Electronic address: pandarun@aliyun.com.
authors.forename: Liyang
authors.initials: L
authors.lastname: Gao
authors.affiliation: Stem Cell Research Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China.
authors.forename: Mingyan
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Obstetrics and Gynecology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ye
authors.affiliation: Obstetrics and Gynecology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China.
authors.forename: Jinzhi
authors.initials: J
authors.lastname: Huang
authors.affiliation: Stem Cell Research Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China.
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Chu
authors.affiliation: Stem Cell Research Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China.
authors.forename: Shouquan
authors.initials: S
authors.lastname: Yan
authors.affiliation: Department of Spinal Surgery, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China.
authors.forename: Chaojun
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Spinal Surgery, Affiliated Hospital of Guangdong Medical University, Zhanjiang, China. Electronic address: wsgly@yahoo.com.
authors.forename: Rong
authors.initials: R
authors.lastname: Zeng
mesh_terms: D000653:Amniotic Fluid / Q000166:cytology*; D002454:Cell Differentiation / Q000235:genetics*; D049109:Cell Proliferation / Q000187:drug effects; D018507:Gene Expression Regulation, Developmental; D038362:Glycogen Synthase Kinase 3 / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D006801:Humans; D007211:Indoles / Q000008:administration & dosage; D008301:Maleimides / Q000008:administration & dosage; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D033581:Stem Cell Transplantation
chemical_list: D007211:Indoles; D008301:Maleimides; C417521:SB 216763; D038362:Glycogen Synthase Kinase 3
keywords: CD117; GSK3 inhibitor; Human amniotic fluid-derived stem cells; Neural differentiation; Neural precursor cells