title: Effects of Growth Hormone on In Situ Culture of Bovine Preantral Follicles are Dose Dependent.
abstract: The objective of this study was to evaluate different concentrations of growth hormone (GH) on the development of bovine preantral follicles cultured included in the ovarian tissue (in situ) on the rates of morphologically normal, viable, primordial and developing follicles, as well as the oocyte and follicle diameter and ultrastructural analysis. Ovarian fragments collected from cows with no cross-breeds defined were cultured in situ for 1 and 7 days in minimal essential medium (α-MEM+) supplemented with different concentrations of recombinant human GH (0, 10, 25, 50 ng/ml). The ovarian fragments non-cultured (control) and cultured were processed for classic histology, mechanical isolation and electron transmission microscopy (MET). The parameters underwent anova (Tukey's and Dunnett's tests) and chi-square test (χ(2) ). After 7 days of culture, the treatment with 50 ng/ml GH showed no differences with fresh control (p > 0.05) and had greater effectiveness than in the 0, 10 and 25 ng/ml GH concentrations of the morphologically normal follicles. Regarding the primordial follicles, a reduction was observed in the 50 ng/ml GH concentration concomitant with the significant increase in developing follicles, differing from both the fresh control and the other GH concentrations tested. In addition, 50 ng/ml GH showed a larger follicle and oocyte diameter when compared to the other treatments cultured. Similar structures were ultrastructurally observed in the control group, 50 ng/ml GH. Follicles cultured in 10 ng/ml GH showed nuclear invagination, vacuoles and lesioned basal membrane. Hence, it is concluded that 50 ng/ml GH is the most effective concentration for the development of preantral follicles cultured in situ.
authors.affiliation: Departament of Animal Science, Laboratory of Animal Physiology and Reproduction, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
authors.forename: C R
authors.initials: CR
authors.lastname: Jimenez
authors.affiliation: Departament of Animal Science, Laboratory of Animal Physiology and Reproduction, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
authors.forename: J L
authors.initials: JL
authors.lastname: de Azevedo
authors.affiliation: Departament of Animal Science, Laboratory of Animal Physiology and Reproduction, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
authors.forename: R G
authors.initials: RG
authors.lastname: Silveira
authors.affiliation: Departament of Animal Science, Laboratory of Animal Physiology and Reproduction, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
authors.forename: J
authors.initials: J
authors.lastname: Penitente-Filho
authors.affiliation: Departament of Animal Science, Laboratory of Animal Physiology and Reproduction, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
authors.forename: E L
authors.initials: EL
authors.lastname: Carrascal-Triana
authors.affiliation: Departament of Animal Science, Laboratory of Animal Physiology and Reproduction, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
authors.forename: A M
authors.initials: AM
authors.lastname: Zolini
authors.affiliation: Laboratory of Manipulation of Oocytes Enclosed in Preantral Follicles, Universidade Estadual do Ceara, Fortaleza, CE, Brazil.
authors.forename: V R
authors.initials: VR
authors.lastname: Araújo
authors.affiliation: Departament of Animal Science, Laboratory of Animal Physiology and Reproduction, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
authors.forename: Caa
authors.initials: C
authors.lastname: Torres
mesh_terms: D000818:Animals; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D005260:Female; D013006:Growth Hormone / Q000008:administration & dosage / Q000494:pharmacology*; D006080:Ovarian Follicle / Q000187:drug effects* / Q000648:ultrastructure; D013997:Time Factors; D046509:Tissue Culture Techniques
chemical_list: D013006:Growth Hormone