title: Single-Photon Emission Computed Tomography for Preclinical Assessment of Thyroid Radioiodide Uptake Following Various Combinations of Preparative Measures.
abstract: MicroSPECT/CT imaging was used to quantitatively evaluate how iodide uptake in the mouse thyroid is influenced by (i) route of iodine administration; (ii) injection of recombinant human thyrotropin (rhTSH); and (iii) low iodide diet (LID) in euthyroid and triiodothyronine (T3)-treated mice.
  
  
  
  Pertechnetate (99mTcO4-) and123I thyroid uptake in euthyroid and T3-treated animals fed either a normal-iodine diet (NID) or an LID, treated or not with rhTSH, and radiotracer administered intravenously, subcutaneously, intraperitoneally or by gavage, were assessed using microSPECT/CT imaging. Western blotting was performed to measure sodium/iodide symporter expression levels in the thyroid.
  
  
  
  Systemic administration of radioiodide resulted in a higher (2.35-fold in NID mice) accumulation of iodide in the thyroid than oral administration. Mice fed LID with systemic radioiodide administration showed a further two-fold increase in thyroid iodide uptake to yield a ∼5-fold increase in uptake compared to the standard NID/oral route. Although rhTSH injections stimulated thyroid activity in both euthyroid and T3-treated mice fed the NID, uptake levels for T3-treated mice remained low compared with those for the euthyroid mice. Combining LID and rhTSH in T3-treated mice resulted in a 2.8-fold higher uptake compared with NID/T3/rhTSH mice and helped restore thyroid activity to levels equivalent to those of euthyroid animals.
  
  
  
  Systemic radioiodide administration results in higher thyroidal iodide levels than oral administration, particularly in LID-fed mice. These data highlight the importance of LID, both in euthyroid and T3-treated, rhTSH-injected mice. Extrapolated to human patients, and in the context of clinical guidelines for the preparation of differentiated thyroid cancer patients, our data indicate that LID can potentiate the efficacy of rhTSH treatment in T3-treated patients.
authors.affiliation: 1 Laboratory of Transporters, Imaging, Radiotherapy in Oncology, Unité Mixte de Recherche E4320, Institut de Biosciences et Biotechnologies , CEA, Nice, France .|2 Université de Nice-Sophia Antipolis , Nice, France .|3 Department of Nuclear Medicine, Centre Antoine Lacassagne , Nice, France .
authors.forename: Colette
authors.initials: C
authors.lastname: Zwarthoed
authors.affiliation: 1 Laboratory of Transporters, Imaging, Radiotherapy in Oncology, Unité Mixte de Recherche E4320, Institut de Biosciences et Biotechnologies , CEA, Nice, France .|2 Université de Nice-Sophia Antipolis , Nice, France .
authors.forename: Kaouthar
authors.initials: K
authors.lastname: Chatti
authors.affiliation: 1 Laboratory of Transporters, Imaging, Radiotherapy in Oncology, Unité Mixte de Recherche E4320, Institut de Biosciences et Biotechnologies , CEA, Nice, France .|2 Université de Nice-Sophia Antipolis , Nice, France .
authors.forename: Julien
authors.initials: J
authors.lastname: Guglielmi
authors.affiliation: 1 Laboratory of Transporters, Imaging, Radiotherapy in Oncology, Unité Mixte de Recherche E4320, Institut de Biosciences et Biotechnologies , CEA, Nice, France .|2 Université de Nice-Sophia Antipolis , Nice, France .
authors.forename: Maha
authors.initials: M
authors.lastname: Hichri
authors.affiliation: 1 Laboratory of Transporters, Imaging, Radiotherapy in Oncology, Unité Mixte de Recherche E4320, Institut de Biosciences et Biotechnologies , CEA, Nice, France .|2 Université de Nice-Sophia Antipolis , Nice, France .
authors.forename: Cathy
authors.initials: C
authors.lastname: Compin
authors.affiliation: 1 Laboratory of Transporters, Imaging, Radiotherapy in Oncology, Unité Mixte de Recherche E4320, Institut de Biosciences et Biotechnologies , CEA, Nice, France .|2 Université de Nice-Sophia Antipolis , Nice, France .|3 Department of Nuclear Medicine, Centre Antoine Lacassagne , Nice, France .
authors.forename: Jacques
authors.initials: J
authors.lastname: Darcourt
authors.affiliation: 1 Laboratory of Transporters, Imaging, Radiotherapy in Oncology, Unité Mixte de Recherche E4320, Institut de Biosciences et Biotechnologies , CEA, Nice, France .|2 Université de Nice-Sophia Antipolis , Nice, France .
authors.forename: Georges
authors.initials: G
authors.lastname: Vassaux
authors.affiliation: 3 Department of Nuclear Medicine, Centre Antoine Lacassagne , Nice, France .
authors.forename: Danielle
authors.initials: D
authors.lastname: Benisvy
authors.affiliation: 1 Laboratory of Transporters, Imaging, Radiotherapy in Oncology, Unité Mixte de Recherche E4320, Institut de Biosciences et Biotechnologies , CEA, Nice, France .|2 Université de Nice-Sophia Antipolis , Nice, France .
authors.forename: Thierry
authors.initials: T
authors.lastname: Pourcher
authors.affiliation: 1 Laboratory of Transporters, Imaging, Radiotherapy in Oncology, Unité Mixte de Recherche E4320, Institut de Biosciences et Biotechnologies , CEA, Nice, France .|2 Université de Nice-Sophia Antipolis , Nice, France .
authors.forename: Béatrice
authors.initials: B
authors.lastname: Cambien
mesh_terms: D000284:Administration, Oral; D000818:Animals; D004032:Diet / Q000009:adverse effects; D005260:Female; D020249:Hormone Replacement Therapy / Q000009:adverse effects; D006801:Humans; D007274:Injections, Intraperitoneal; D007275:Injections, Intravenous; D007279:Injections, Subcutaneous; D007455:Iodine / Q000008:administration & dosage / Q000009:adverse effects; D007457:Iodine Radioisotopes / Q000008:administration & dosage / Q000378:metabolism / Q000493:pharmacokinetics*; D008810:Mice, Inbred C57BL; D019275:Radiopharmaceuticals / Q000008:administration & dosage / Q000378:metabolism / Q000493:pharmacokinetics*; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000009:adverse effects / Q000494:pharmacology; D013670:Sodium Pertechnetate Tc 99m / Q000378:metabolism / Q000493:pharmacokinetics; D013961:Thyroid Gland / Q000000981:diagnostic imaging* / Q000187:drug effects / Q000378:metabolism; D013972:Thyrotropin / Q000008:administration & dosage / Q000009:adverse effects / Q000494:pharmacology; D014018:Tissue Distribution; D015899:Tomography, Emission-Computed, Single-Photon; D014284:Triiodothyronine / Q000008:administration & dosage / Q000378:metabolism / Q000493:pharmacokinetics*
chemical_list: D007457:Iodine Radioisotopes; D019275:Radiopharmaceuticals; D011994:Recombinant Proteins; D014284:Triiodothyronine; D013972:Thyrotropin; D007455:Iodine; D013670:Sodium Pertechnetate Tc 99m
keywords: differentiated thyroid carcinoma; hormone replacement therapy; iodine administration route; microSPECT/CT; radioiodine therapy; recombinant human TSH