title: Anticoagulant Activity and Structural Characterization of Polysaccharide from Abalone (Haliotis discus hannai Ino) Gonad.
abstract: In this study, we aimed at characterizing the structure and the anticoagulant activity of a polysaccharide fraction (AGP33) isolated from the gonads of Haliotis discus hannai Ino. AGP33 was extracted by enzymatic hydrolysis and purified by ion-exchange and gel-filtration chromatography. The backbone fraction of AGP33 (BAGP33), which appeared to contain of mannose, glucose and galactose, was prepared by partial acid hydrolysis. According to methylation and nuclear magnetic resonance (NMR) spectroscopy, the backbone of AGP33 was identified as mainly consisting of 1→3-linked, 1→4-linked, and 1→6-linked monosaccharides. AGP33 is a sulfated polysaccharide with sulfates occur at 3-O- and 4-O-positions. It prolonged thromboplastin time (APTT), thrombin time (TT) and prothrombin time (PT) compared to a saline control solution in a dosage-dependent manner. AGP33 exhibited an extension (p < 0.01) of APTT compared to the saline group at concentrations higher than 5 μg/mL. AGP33 exhibited higher anticoagulant activity than its desulfated product (AGP33-des) and BAGP33. The results showed that polysaccharide with higher molecular weight and sulfate content demonstrated greater anticoagulant activity.
authors.affiliation: School of Light Industry and Chemical Engineering, Dalian Polytechnic University, Dalian 116034, China. kittyufo@163.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
authors.affiliation: School of Food Science and Technology, National Engineering Research Center of Seafood, National and Local Joint Engineering Laboratory for Marine Bioactive Polysaccharide Development and Application, Dalian Polytechnic University, Dalian 116034, China. yangjf@dlpu.edu.cn.
authors.forename: Jingfeng
authors.initials: J
authors.lastname: Yang
authors.affiliation: School of Food Science and Technology, National Engineering Research Center of Seafood, National and Local Joint Engineering Laboratory for Marine Bioactive Polysaccharide Development and Application, Dalian Polytechnic University, Dalian 116034, China. songs1008@163.com.
authors.forename: Shuang
authors.initials: S
authors.lastname: Song
authors.affiliation: School of Food Science and Technology, National Engineering Research Center of Seafood, National and Local Joint Engineering Laboratory for Marine Bioactive Polysaccharide Development and Application, Dalian Polytechnic University, Dalian 116034, China. zdyzf1@163.com.
authors.forename: Dayong
authors.initials: D
authors.lastname: Zhou
authors.affiliation: Clinical Laboratory, Dalian Municipal Central Hospital Affiliated of Dalian Medical University, Dalian 116033, China. qiaoweizhouzxyy@163.com.
authors.forename: Weizhou
authors.initials: W
authors.lastname: Qiao
authors.affiliation: School of Food Science and Technology, National Engineering Research Center of Seafood, National and Local Joint Engineering Laboratory for Marine Bioactive Polysaccharide Development and Application, Dalian Polytechnic University, Dalian 116034, China. silvia8801@163.com.
authors.forename: Ce
authors.initials: C
authors.lastname: Zhu
authors.affiliation: School of Food Science and Technology, National Engineering Research Center of Seafood, National and Local Joint Engineering Laboratory for Marine Bioactive Polysaccharide Development and Application, Dalian Polytechnic University, Dalian 116034, China. 18340801443@163.com.
authors.forename: Shuyin
authors.initials: S
authors.lastname: Liu
authors.affiliation: School of Food Science and Technology, National Engineering Research Center of Seafood, National and Local Joint Engineering Laboratory for Marine Bioactive Polysaccharide Development and Application, Dalian Polytechnic University, Dalian 116034, China. zhubeiwei@163.com.
authors.forename: Beiwei
authors.initials: B
authors.lastname: Zhu
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000737:chemistry / Q000378:metabolism*; D005690:Galactose / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D006066:Gonads / Q000737:chemistry; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008358:Mannose / Q000737:chemistry; D008745:Methylation; D008974:Mollusca / Q000737:chemistry* / Q000378:metabolism; D010314:Partial Thromboplastin Time; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D011517:Prothrombin Time; D013329:Structure-Activity Relationship; D013918:Thrombin Time
chemical_list: D000925:Anticoagulants; D011134:Polysaccharides; D005947:Glucose; D008358:Mannose; D005690:Galactose
keywords: APTT; Haliotis discus hannai; PT; TT; backbone; polysaccharide