title: Drug-induced Modulation of Heat Shock Protein HSPB1 in an Ovarian Cancer Cell Model.
abstract: BACKGROUND
  Heat-shock protein HSPB1 (alternative name HSP27) plays a pivotal role in cell survival pathways, apoptosis, metastasis and has been frequently linked to treatment resistance in ovarian cancer (OC) and other malignancies. Characteristic HSPB1 induction in different solid tumors is often caused by cytotoxic agents.
  
  
  METHODS
  An in vitro OC cell model system was established to characterize resistance mechanisms during chemotherapy. Human OC cell lines OVCAR-3, SK-OV-3 and TOV-21G were treated with paclitaxel or carboplatin. Cellular growth was analyzed by cell counting. Intra- and extracellular HSPB1 concentrations were assessed by western blot and enzyme-linked immunosorbent assays.
  
  
  RESULTS
  Incubation with paclitaxel, and with carboplatin significantly reduced cell growth without a definitive increase of intracellular HSPB1 expression. HSPB1 demonstrated drug-inducible secretion into the extracellular compartment.
  
  
  CONCLUSIONS
  Despite its current lack of analysis in patient samples, serum soluble HSPB1 may function as a specific biomarker for monitoring response to chemotherapy in patients with OC.
authors.affiliation: Department of Urology, University of Medicine Greifswald, Greifswald, Germany matthias.stope@uni-greifswald.de.
authors.forename: Matthias B
authors.initials: MB
authors.lastname: Stope
authors.affiliation: Department of Obstetrics and Gynaecology, University of Medicine Greifswald, Greifswald, Germany.
authors.forename: Luise
authors.initials: L
authors.lastname: Wiegank
authors.affiliation: Department of Urology, University of Medicine Greifswald, Greifswald, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Weiss
authors.affiliation: Department of Obstetrics and Gynaecology, University of Medicine Greifswald, Greifswald, Germany.
authors.forename: Karoline
authors.initials: K
authors.lastname: Diesing
authors.affiliation: Department of Obstetrics and Gynaecology, University of Medicine Greifswald, Greifswald, Germany.
authors.forename: Dominique
authors.initials: D
authors.lastname: Koensgen
authors.affiliation: Department of Urology, University of Medicine Greifswald, Greifswald, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Burchardt
authors.affiliation: Department of Obstetrics and Gynaecology, University of Medicine Greifswald, Greifswald, Germany.
authors.forename: Marek
authors.initials: M
authors.lastname: Zygmunt
authors.affiliation: Department of Obstetrics and Gynaecology, University of Medicine Greifswald, Greifswald, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Mustea
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D015153:Blotting, Western; D016190:Carboplatin / Q000494:pharmacology*; D048708:Cell Growth Processes / Q000187:drug effects; D045744:Cell Line, Tumor; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D055551:HSP27 Heat-Shock Proteins / Q000096:biosynthesis*; D006360:Heat-Shock Proteins; D006801:Humans; D018832:Molecular Chaperones; D010051:Ovarian Neoplasms / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology; D017239:Paclitaxel / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D000972:Antineoplastic Agents, Phytogenic; D055551:HSP27 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D016190:Carboplatin; D017239:Paclitaxel
keywords: Ovarian cancer; chemotherapy; heat-shock protein HSPB1; protein secretion