title: Screening and identification of a microsatellite marker associated with sex in Wami tilapia, Oreochromis urolepis hornorum.
abstract: In this study, primer pairs of 15 microsatellite markers associated with sex determination of tilapia were selected and amplified in Wami tilapia, Oreochromis urolepis hornorum. While one marker, UNH168, on linkage group 3 (LG3) was associated (P <0.001) with the phenotypic sex in the experimental population, nine genotypes were detected in both sexes. Only 99-bp allele was detected in the female samples, while 141, 149 and 157-bp alleles were present in both male and female samples. UNH168 was localized by fluorescence in situ hybridization (FISH) on the long arm of the largest tilapia chromosome pair (chromosome 1, equivalent to LG3). This sex-linked microsatellite marker could potentially be used for marker-assisted selection in tilapia breeding programmes to produce monosex male tilapia.
authors.affiliation: Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, People's Republic of China.zhhping2000@163.com.
authors.forename: Huaping
authors.initials: H
authors.lastname: Zhu
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Liu
authors.forename: Maixin
authors.initials: M
authors.lastname: Lu
authors.forename: Fengying
authors.initials: F
authors.lastname: Gao
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Ke
authors.forename: Dongmei
authors.initials: D
authors.lastname: Ma
authors.forename: Zhanghan
authors.initials: Z
authors.lastname: Huang
authors.forename: Jianmeng
authors.initials: J
authors.lastname: Cao
authors.forename: Miao
authors.initials: M
authors.lastname: Wang
mesh_terms: D000483:Alleles; D000818:Animals; D001947:Breeding; D002874:Chromosome Mapping; D002875:Chromosomes / Q000737:chemistry*; D005260:Female; D008040:Genetic Linkage; D005819:Genetic Markers; D005838:Genotype; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D018895:Microsatellite Repeats; D010641:Phenotype; D019849:Sex Determination Processes; D017210:Tilapia / Q000235:genetics*
chemical_list: D005819:Genetic Markers