title: Antifungal Activity of Diglycerin Ester of Fatty Acids against Yeasts and Its Comparison with Those of Sucrose Monopalmitate and Sodium Benzoate.
abstract: The antifungal activities of diglycerin monoester of fatty acids (DGCs), which have been employed as food emulsifiers, were examined against three yeasts, Saccharomyces cerevisiae, Candida albicans and Candida utilis and were compared with those of sucrose monoester of palmitic acid (SC16) as another type of emulsifier and sodium benzoate (SB) as a weak acid food preservative. When the minimum growth inhibitory concentrations (MICs) of diglycerin monolaurate (DGC12) against these yeasts were determined 2 d after incubation in YM broth at pH5.0, they were relatively low, being 0.01% (w/v), for both S. cerevisiae and C. utilis, whereas was high, being 4.0% (w/v), for C. albicans. On the contrary, the MICs of sucrose monopalmitate (SC16) were high, being 3.0 and 4.0% (w/v), for the former two yeasts, respectively, but 0.6% (w/v) for the last yeast. In contrast to these emulsifiers, the MICs of sodium benzoate (SB) were similar independently upon the yeast strain, being in order 0.4, 0.3 and 0.5% (w/v), for the above yeasts, respectively. The anti-yeast activities of DGC12 and SC16 were gradually increased with a decrease in pH, in a manner similar to that of SB, except for the action of SC16 on C. albicans, for which the activity was more effective at pHs 5.0 and 6.0 than at pHs 4.0 and 7.0. Among DGCs tested having different fatty acid moieties in the molecule, lauroyl ester (DGC12) was more effective than myristoyl and palmitoyl esters against S. cerevisiae and C. utilis. The inhibitory effect of DGC12 on the yeast growth depended upon both the cell density and the strength of aeration during the treatment. Further, DGC12 was found to kill S. cerevisiae and C. utilis cells at a rather low concentration of 0.005% (w/v) in 50mM acetate buffer at pH5.0, although, against C. albicans cells, only slight fungicidal activity was demonstrated at a high concentration of 0.5% (w/v). The results obtained support the effectiveness of practical application of DGC12 to acidic foods for the control of growth and survival of general yeasts such as S. cerevisiae and C. utilis.
authors.affiliation: Department of Biotechnology, Faculty of Engineering, Kansai University.
authors.forename: Aiko
authors.initials: A
authors.lastname: Shimazaki
authors.forename: Jin J
authors.initials: JJ
authors.lastname: Sakamoto
authors.forename: Masakazu
authors.initials: M
authors.lastname: Furuta
authors.forename: Tetsuaki
authors.initials: T
authors.lastname: Tsuchido
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects; D002176:Candida albicans / Q000187:drug effects; D004952:Esters / Q000494:pharmacology*; D005227:Fatty Acids; D006863:Hydrogen-Ion Concentration; D008826:Microbial Sensitivity Tests; D012441:Saccharomyces cerevisiae / Q000187:drug effects; D020160:Sodium Benzoate / Q000494:pharmacology*; D013395:Sucrose / Q000031:analogs & derivatives* / Q000494:pharmacology; D015003:Yeasts / Q000187:drug effects*
chemical_list: D000935:Antifungal Agents; D004952:Esters; D005227:Fatty Acids; C041567:sucrose monopalmitate; D013395:Sucrose; D020160:Sodium Benzoate