title: High-quality permanent draft genome sequence of Ensifer sp. PC2, isolated from a nitrogen-fixing root nodule of the legume tree (Khejri) native to the Thar Desert of India.
abstract: Ensifer sp. PC2 is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from a nitrogen-fixing nodule of the tree legume P. cineraria (L.) Druce (Khejri), which is a keystone species that grows in arid and semi-arid regions of the Indian Thar desert. Strain PC2 exists as a dominant saprophyte in alkaline soils of Western Rajasthan. It is fast growing, well-adapted to arid conditions and is able to form an effective symbiosis with several annual crop legumes as well as species of mimosoid trees and shrubs. Here we describe the features of Ensifer sp. PC2, together with genome sequence information and its annotation. The 8,458,965 bp high-quality permanent draft genome is arranged into 171 scaffolds of 171 contigs containing 8,344 protein-coding genes and 139 RNA-only encoding genes, and is one of the rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project proposal.
authors.affiliation: BNF and Stress Biology Lab., Department of Botany, J.N. Vyas University, Jodhpur, 342001 India.
authors.forename: Hukam Singh
authors.initials: HS
authors.lastname: Gehlot
authors.affiliation: Centre for Studies, Murdoch University, Murdoch, Western Australia Australia.
authors.forename: Julie
authors.initials: J
authors.lastname: Ardley
authors.affiliation: BNF and Stress Biology Lab., Department of Botany, J.N. Vyas University, Jodhpur, 342001 India.
authors.forename: Nisha
authors.initials: N
authors.lastname: Tak
authors.affiliation: Centre for Studies, Murdoch University, Murdoch, Western Australia Australia.
authors.forename: Rui
authors.initials: R
authors.lastname: Tian
authors.affiliation: BNF and Stress Biology Lab., Department of Botany, J.N. Vyas University, Jodhpur, 342001 India.
authors.forename: Neetu
authors.initials: N
authors.lastname: Poonar
authors.affiliation: BNF and Stress Biology Lab., Department of Botany, J.N. Vyas University, Jodhpur, 342001 India.
authors.forename: Raju R
authors.initials: RR
authors.lastname: Meghwal
authors.affiliation: BNF and Stress Biology Lab., Department of Botany, J.N. Vyas University, Jodhpur, 342001 India.
authors.forename: Sonam
authors.initials: S
authors.lastname: Rathi
authors.affiliation: Centre for Studies, Murdoch University, Murdoch, Western Australia Australia.
authors.forename: Ravi
authors.initials: R
authors.lastname: Tiwari
authors.affiliation: Centre for Studies, Murdoch University, Murdoch, Western Australia Australia.
authors.forename: Wan
authors.initials: W
authors.lastname: Adnawani
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California USA.
authors.forename: Rekha
authors.initials: R
authors.lastname: Seshadri
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California USA.
authors.forename: T B K
authors.initials: TB
authors.lastname: Reddy
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California USA.
authors.forename: Amrita
authors.initials: A
authors.lastname: Pati
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California USA.
authors.forename: Tanja
authors.initials: T
authors.lastname: Woyke
authors.affiliation: Biological Data Management and Technology Center, Lawrence Berkeley National Laboratory, Berkeley, California USA.
authors.forename: Manoj
authors.initials: M
authors.lastname: Pillay
authors.affiliation: Biological Data Management and Technology Center, Lawrence Berkeley National Laboratory, Berkeley, California USA.
authors.forename: Victor
authors.initials: V
authors.lastname: Markowitz
authors.affiliation: Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Mohammed N
authors.initials: MN
authors.lastname: Baeshen
authors.affiliation: Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Ahmed M
authors.initials: AM
authors.lastname: Al-Hejin
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California USA.
authors.forename: Natalia
authors.initials: N
authors.lastname: Ivanova
authors.affiliation: DOE Joint Genome Institute, Walnut Creek, California USA ; Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
authors.forename: Nikos
authors.initials: N
authors.lastname: Kyrpides
authors.affiliation: Centre for Studies, Murdoch University, Murdoch, Western Australia Australia.
authors.forename: Wayne
authors.initials: W
authors.lastname: Reeve
keywords: Ensifer; Nitrogen fixation; Prosopis; Root-nodule bacteria; Symbiosis