title: Role of microRNA-4516 involved autophagy associated with exposure to fine particulate matter.
abstract: Metals are vital toxic components of fine particulate matter (PM2.5). Cellular responses to exposure to PM2.5 or PM metal components remain unknown. Post-transcriptional profiling and subsequent cell- and individual-based assays implied that the metal ion-binding miR-4516/RPL37/autophagy pathway could play a critical role in cellular responses to PM2.5 and PM metal stresses. miR-4516 was up-regulated in A549 cells exposed to PM2.5 and in the serum of individuals living in a city with moderate air pollution. The expression levels of the miR-4516 target genes, namely, RPL37 and UBA52, were involved in ribosome function and inhibited by exposure to PM2.5 and PM metal components. Autophagy in A549 cells was induced by PM2.5 exposure as a response to decreased RPL37 expression. Moreover, enhanced miR-4516 expression was positively correlated with the augmentation of the internal burden of aluminum and lead in individuals living in a city with moderate air pollution. Hereby, the miR-4516/RPL37/autophagy pathway may represent a novel mechanism that mediates responses to PM metal components.
authors.affiliation: Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Histology and Embryology, Hebei North University, Zhangjiakou 075000, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Lv
authors.affiliation: Clinical Laboratory of The Second Hospital, Hebei Medical University, Shijiazhuang 050000, China.
authors.forename: Jihong
authors.initials: J
authors.lastname: Hao
authors.affiliation: Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Sun
authors.affiliation: Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China.
authors.forename: Na
authors.initials: N
authors.lastname: Gao
authors.affiliation: Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China.
authors.forename: Chengcheng
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China.
authors.forename: Runze
authors.initials: R
authors.lastname: Lu
authors.affiliation: Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China.
authors.forename: Shizhi
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Yin
authors.affiliation: Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China.
authors.forename: Yuepu
authors.initials: Y
authors.lastname: Pu
authors.affiliation: Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China.|State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Chen
mesh_terms: D000328:Adult; D001343:Autophagy / Q000502:physiology*; D019295:Computational Biology; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000032:analysis / Q000502:physiology*; D008875:Middle Aged; D052638:Particulate Matter / Q000633:toxicity*; D012269:Ribosomal Proteins / Q000235:genetics
chemical_list: C000591812:MIRN-4516 microRNA, human; D035683:MicroRNAs; D052638:Particulate Matter; C488984:RPL37 protein, human; D012269:Ribosomal Proteins
keywords: PM2.5; autophagy; metal; microRNA; ribosome