title: Structural characterization of the lipid A from the LPS of the haloalkaliphilic bacterium Halomonas pantelleriensis.
abstract: Halomonas pantelleriensis DSM9661(Τ) is a Gram-negative haloalkaliphilic bacterium isolated from the sand of the volcanic Venus mirror lake, closed to seashore in the Pantelleria Island in the south of Italy. It is able to optimally grow in media containing 3-15 % (w/v) total salt and at pH between 9 and 10. To survive in these harsh conditions, the bacterium has developed several strategies that probably concern the bacteria outer membrane, a barrier regulating the exchange with the environment. In such a context, the lipopolysaccharides (LPSs), which are among the major constituent of the Gram-negative outer membrane, are thought to contribute to the restrictive membrane permeability properties. The structure of the lipid A family derived from the LPS of Halomonas pantelleriensis DSM 9661(T) is reported herein. The lipid A was obtained from the purified LPS by mild acid hydrolysis. The lipid A, which contains different numbers of fatty acids residues, and its partially deacylated derivatives were completely characterized by means of ESI FT-ICR mass spectrometry and chemical analysis. Preliminary immunological assays were performed, and a comparison with the lipid A structure of the phylogenetic proximal Halomonas magadiensis is also reported.
authors.affiliation: National Institute for Bioprocessing Research and Training, Fosters Avenue, Mount Merrion, Blackrock, County Dublin, Ireland.
authors.forename: Sara
authors.initials: S
authors.lastname: Carillo
authors.affiliation: Dipartimento di Scienze Chimiche, Università di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126, Naples, Italy.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Pieretti
authors.affiliation: Dipartimento di Scienze Chimiche, Università di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126, Naples, Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Casillo
authors.affiliation: Division of Bioanalytical Chemistry, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Parkallee 10, 23845, Borstel, Germany.
authors.forename: Buko
authors.initials: B
authors.lastname: Lindner
authors.affiliation: Istituto di Chimica Biomolecolare ICB-CNR, Via Campi Flegrei 34, 80078, Pozzuoli, Italy.
authors.forename: Ida
authors.initials: I
authors.lastname: Romano
authors.affiliation: Istituto di Chimica Biomolecolare ICB-CNR, Via Campi Flegrei 34, 80078, Pozzuoli, Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Nicolaus
authors.affiliation: Department of Biology, University of Naples "Federico II", Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126, Naples, Italy.
authors.forename: Michelangelo
authors.initials: M
authors.lastname: Parrilli
authors.affiliation: Department of Experimental Medicine, Second University of Naples, via de Crecchio 7, 80138, Naples, Italy.
authors.forename: Mariateresa
authors.initials: M
authors.lastname: Giuliano
authors.affiliation: Department of Experimental Medicine, Second University of Naples, via de Crecchio 7, 80138, Naples, Italy.
authors.forename: Marcella
authors.initials: M
authors.lastname: Cammarota
authors.affiliation: Dipartimento di Scienze Chimiche, Università di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126, Naples, Italy.
authors.forename: Rosa
authors.initials: R
authors.lastname: Lanzetta
authors.affiliation: Dipartimento di Scienze Chimiche, Università di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126, Naples, Italy. corsaro@unina.it.
authors.forename: Maria Michela
authors.initials: MM
authors.lastname: Corsaro
mesh_terms: D045744:Cell Line, Tumor; D020632:Halomonas / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008050:Lipid A / Q000737:chemistry* / Q000276:immunology
chemical_list: D008050:Lipid A
keywords: Extremophile; Haloalkaliphilic; Halomonas; Lipid A; Mass spectrometry