title: Adiponectin corrects premature cellular senescence and normalizes antimicrobial peptide levels in senescent keratinocytes.
abstract: Stress-induced premature senescence or aging causes dysfunction in the human somatic system. Adiponectin (Acrp30) plays a role in functional recovery, especially with adenosine 3',5'-monophosphate (AMP)-activated protein kinase (AMPK) and silent mating type information regulation 2 homolog 1 (SIRT1). Acrp30 stimulation reduced the premature senescence positive ratio induced by hydrogen peroxide (H2O2) and restituted human β-defensin 2 (hBD-2) levels in senescent keratinocytes. Acrp30 recovered AMPK activity in senescent keratinocytes and increased SIRT1 deacetylation activity. As a result, FoxO1 and FoxO3 transcription activity was recovered. Additionally, Acrp30 stimulation suppresses NFκB p65, which induces abnormal expression of hBD-2 induced by H2O2. In the present study, we have shown that Acrp30 reduces premature senescence and recovers cellular function in keratinocytes. These results suggest a role for Acrp30 as an anti-aging agent to improve impaired skin immune barriers.
authors.affiliation: Department of Dermatology, Chung-Ang University Hospital, Seoul, South Korea. Electronic address: j.magma@gmail.com.
authors.forename: Taewon
authors.initials: T
authors.lastname: Jin
authors.affiliation: Department of Dermatology, Chung-Ang University Hospital, Seoul, South Korea.
authors.forename: Min Jeong
authors.initials: MJ
authors.lastname: Kim
authors.affiliation: Department of Dermatology, Chung-Ang University Hospital, Seoul, South Korea.
authors.forename: Won Il
authors.initials: WI
authors.lastname: Heo
authors.affiliation: Department of Dermatology, Chung-Ang University Hospital, Seoul, South Korea.
authors.forename: Kui Young
authors.initials: KY
authors.lastname: Park
authors.affiliation: Department of Dermatology, Chung-Ang University Hospital, Seoul, South Korea.
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Choi
authors.affiliation: Department of Laboratory Medicine, Chung-Ang University Hospital, Seoul, South Korea.
authors.forename: Mi-Kyung
authors.initials: MK
authors.lastname: Lee
authors.affiliation: Department of Dermatology, College of Medicine, Dankook University, Cheonan, South Korea.
authors.forename: Seung-Phil
authors.initials: SP
authors.lastname: Hong
authors.affiliation: Department of Dermatology, Chonnam National University Medical School, Gwangju, South Korea.
authors.forename: Seong-Jin
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Department of Dermatology, Chungnam National University School of Medicine, Daejeon, South Korea.
authors.forename: Myung
authors.initials: M
authors.lastname: Im
authors.affiliation: Department of Ophthalmology, Chung-Ang University Hospital, Seoul, South Korea.
authors.forename: Nam Ju
authors.initials: NJ
authors.lastname: Moon
authors.affiliation: Department of Dermatology, Chung-Ang University Hospital, Seoul, South Korea. Electronic address: drseo@cau.ac.kr.
authors.forename: Seong Jun
authors.initials: SJ
authors.lastname: Seo
mesh_terms: D052242:Adiponectin / Q000378:metabolism*; D023181:Antimicrobial Cationic Peptides / Q000378:metabolism*; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000008:administration & dosage; D015603:Keratinocytes / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D013312:Stress, Physiological / Q000187:drug effects / Q000502:physiology*
chemical_list: D052242:Adiponectin; D023181:Antimicrobial Cationic Peptides; D006861:Hydrogen Peroxide
keywords: Adiponectin; Antimicrobial peptide; Keratinocyte; SIRT1; Senescence