title: Targeting long non-coding RNA-TUG1 inhibits tumor growth and angiogenesis in hepatoblastoma.
abstract: Hepatoblastoma is the most common liver tumor of early childhood, which is usually characterized by unusual hypervascularity. Recently, long non-coding RNAs (lncRNA) have emerged as gene regulators and prognostic markers in several cancers, including hepatoblastoma. We previously reveal that lnRNA-TUG1 is upregulated in hepatoblastoma specimens by microarray analysis. In this study, we aim to elucidate the biological and clinical significance of TUG1 upregulation in hepatoblastoma. We show that TUG1 is significantly upregulated in human hepatoblastoma specimens and metastatic hepatoblastoma cell lines. TUG1 knockdown inhibits tumor growth and angiogenesis in vivo, and decreases hepatoblastoma cell viability, proliferation, migration, and invasion in vitro. TUG1, miR-34a-5p, and VEGFA constitutes to a regulatory network, and participates in regulating hepatoblastoma cell function, tumor progression, and tumor angiogenesis. Overall, our findings indicate that TUG1 upregulation contributes to unusual hypervascularity of hepatoblastoma. TUG1 is a promising therapeutic target for aggressive, recurrent, or metastatic hepatoblastoma.
authors.affiliation: Department of Pediatric Surgery, Children's Hospital of Fudan University, Shanghai, China.|Shanghai Key Laboratory of Birth Defect, Shanghai, China.|Key Laboratory of Neonatal Disease, Ministry of Health, Shanghai, China.
authors.forename: R
authors.initials: R
authors.lastname: Dong
authors.affiliation: Department of Pediatric Surgery, Children's Hospital of Fudan University, Shanghai, China.
authors.forename: G-B
authors.initials: GB
authors.lastname: Liu
authors.affiliation: Department of Pediatric Surgery, Children's Hospital of Fudan University, Shanghai, China.
authors.forename: B-H
authors.initials: BH
authors.lastname: Liu
authors.affiliation: Department of Pediatric Surgery, Children's Hospital of Fudan University, Shanghai, China.
authors.forename: G
authors.initials: G
authors.lastname: Chen
authors.affiliation: Department of Pediatric Surgery, Children's Hospital of Fudan University, Shanghai, China.|Shanghai Key Laboratory of Birth Defect, Shanghai, China.|Key Laboratory of Neonatal Disease, Ministry of Health, Shanghai, China.
authors.forename: K
authors.initials: K
authors.lastname: Li
authors.affiliation: Department of Pediatric Surgery, Children's Hospital of Fudan University, Shanghai, China.|Shanghai Key Laboratory of Birth Defect, Shanghai, China.|Key Laboratory of Neonatal Disease, Ministry of Health, Shanghai, China.
authors.forename: S
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Department of Pediatric Surgery, Children's Hospital of Fudan University, Shanghai, China.|Shanghai Key Laboratory of Birth Defect, Shanghai, China.|Key Laboratory of Neonatal Disease, Ministry of Health, Shanghai, China.
authors.forename: K-R
authors.initials: KR
authors.lastname: Dong
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D002470:Cell Survival / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D018197:Hepatoblastoma / Q000098:blood supply* / Q000235:genetics* / Q000473:pathology; D006801:Humans; D008113:Liver Neoplasms / Q000098:blood supply* / Q000235:genetics* / Q000473:pathology; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D009361:Neoplasm Invasiveness; D009389:Neovascularization, Pathologic / Q000235:genetics*; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation / Q000235:genetics; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: C522425:MIRN34 microRNA, human; D035683:MicroRNAs; D062085:RNA, Long Noncoding; C000598536:TUG1 long noncoding RNA, human; D042461:Vascular Endothelial Growth Factor A