title: Identification of QTLs for Resistance to Sclerotinia sclerotiorum in Carioca Common Bean by the Moving Away Method.
abstract: The aim of this study was to use multiple DNA markers for detection of QTLs related to resistance to white mold in an F2 population of common bean evaluated by the straw test method. The DNA from 186 F2 plants and from the parents was extracted for genotypic evaluation using SSR, AFLP, and SRAP markers. For phenotypic analysis, 186 F2:4 progenies and ten lines were evaluated, in a 14 × 14 triple lattice experimental design. The adjusted mean values of the F2:4 progenies were used for identification of QTLs by Bayesian shrinkage analysis. Significant differences were observed among the progenies for reaction to white mold. In identification of QTLs, 17 markers identified QTLs for resistance-13 SSRs and 4 AFLPs. The moving away method under the Bayesian approach proved to be efficient in the identification of QTLs when a genetic map is not used due to the low density of markers. The ME1 and BM211 markers are near the QTLs, with the effect of increasing resistance to white mold, and they have high heritability. They are thus promising for marker-assisted selection.
authors.affiliation: Laboratório Genética Molecular, Departamento de Biologia, Universidade Federal de Lavras, Lavras, MG, Brazil.
authors.forename: Letícia A de C
authors.initials: LA
authors.lastname: Lara
authors.affiliation: Laboratório Genética Molecular, Departamento de Biologia, Universidade Federal de Lavras, Lavras, MG, Brazil.
authors.forename: João B
authors.initials: JB
authors.lastname: Dos Santos
authors.affiliation: Laboratório Genética Molecular, Departamento de Biologia, Universidade Federal de Lavras, Lavras, MG, Brazil.
authors.forename: Juliana S
authors.initials: JS
authors.lastname: Veloso
authors.affiliation: Departamento de Ciências Exatas, Universidade Federal de Lavras, Lavras, MG, Brazil.
authors.forename: Marcio
authors.initials: M
authors.lastname: Balestre
authors.affiliation: Laboratório Genética Molecular, Departamento de Biologia, Universidade Federal de Lavras, Lavras, MG, Brazil.
authors.forename: Filipe C
authors.initials: FC
authors.lastname: Alves
authors.affiliation: Laboratório Genética Molecular, Departamento de Biologia, Universidade Federal de Lavras, Lavras, MG, Brazil.
authors.forename: Monik E
authors.initials: ME
authors.lastname: Leite