title: A partial metabolic pathway enables group b streptococcus to overcome quinone deficiency in a host bacterial community.
abstract: Aerobic respiration metabolism in Group B Streptococcus (GBS) is activated by exogenous heme and menaquinone. This capacity enhances resistance of GBS to acid and oxidative stress and improves its survival. In this work, we discovered that GBS is able to respire in the presence of heme and 1,4-dihydroxy-2-naphthoic acid (DHNA). DHNA is a biosynthetic precursor of demethylmenaquinone (DMK) in many bacterial species. A GBS gene (gbs1789) encodes a homolog of the MenA 1,4-dihydroxy-2-naphthoate prenyltransferase enzyme, involved in the synthesis of demethylmenaquinone. In this study, we showed that gbs1789 is involved in the biosynthesis of long-chain demethylmenaquinones (DMK-10). The Δgbs1789 mutant cannot respire in the presence of heme and DHNA, indicating that endogenously synthesized DMKs are cofactors of the GBS respiratory chain. We also found that isoprenoid side chains from GBS DMKs are produced by the protein encoded by the gbs1783 gene, since this gene can complement an Escherichia coli ispB mutant defective for isoprenoids chain synthesis. In the gut or vaginal microbiote, where interspecies metabolite exchanges occur, this partial DMK biosynthetic pathway can be important for GBS respiration and survival in different niches.
authors.affiliation: Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, 78350, France. thierry.franza@jouy.inra.fr.
authors.forename: Thierry
authors.initials: T
authors.lastname: Franza
authors.affiliation: Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, 78350, France.
authors.forename: Emilie
authors.initials: E
authors.lastname: Delavenne
authors.affiliation: Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, 78350, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Derré-Bobillot
authors.affiliation: Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, 78350, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Juillard
authors.affiliation: Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, 78350, France.
authors.forename: Mylène
authors.initials: M
authors.lastname: Boulay
authors.affiliation: ESPCI Paris, SMBP USR3149 CNRS, Paris, F-75005, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Demey
authors.affiliation: ESPCI Paris, SMBP USR3149 CNRS, Paris, F-75005, France.
authors.forename: Joelle
authors.initials: J
authors.lastname: Vinh
authors.affiliation: Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, 78350, France.
authors.forename: Gilles
authors.initials: G
authors.lastname: Lamberet
authors.affiliation: Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, 78350, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Gaudu
mesh_terms: D016227:Benzoquinones / Q000378:metabolism*; D053898:Biosynthetic Pathways; D006418:Heme / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D009284:Naphthols / Q000378:metabolism / Q000494:pharmacology; D013292:Streptococcus agalactiae / Q000235:genetics / Q000378:metabolism*; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000378:metabolism*
chemical_list: D016227:Benzoquinones; D009284:Naphthols; D024482:Vitamin K 2; C024023:1,4-dihydroxy-2-naphthoic acid; C013917:demethylmenaquinone; C004532:quinone; D006418:Heme