title: Surveying Rubisco Diversity and Temperature Response to Improve Crop Photosynthetic Efficiency.
abstract: The threat to global food security of stagnating yields and population growth makes increasing crop productivity a critical goal over the coming decades. One key target for improving crop productivity and yields is increasing the efficiency of photosynthesis. Central to photosynthesis is Rubisco, which is a critical but often rate-limiting component. Here, we present full Rubisco catalytic properties measured at three temperatures for 75 plants species representing both crops and undomesticated plants from diverse climates. Some newly characterized Rubiscos were naturally "better" compared to crop enzymes and have the potential to improve crop photosynthetic efficiency. The temperature response of the various catalytic parameters was largely consistent across the diverse range of species, though absolute values showed significant variation in Rubisco catalysis, even between closely related species. An analysis of residue differences among the species characterized identified a number of candidate amino acid substitutions that will aid in advancing engineering of improved Rubisco in crop systems. This study provides new insights on the range of Rubisco catalysis and temperature response present in nature, and provides new information to include in models from leaf to canopy and ecosystem scale.
authors.affiliation: Lancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, United Kingdom (D.J.O., A.A., E.C.-S., M.A.J.P.); Rothamsted Research, Plant Biology and Crop Science, Harpenden AL5 2JQ, United Kingdom (D.J.O., A.A., P.J.A., E.C.-S., M.A.J.P.); Plant Sciences Division, Research School of Biology, Australian National University, Canberra, ACT 0200, Australia (M.V.K.); and School of Natural Sciences and Psychology, Liverpool John Moores University, Liverpool L3 3AF, United Kingdom (M.V.K.) d.j.orr@lancaster.ac.uk.
authors.forename: Douglas J
authors.identifier: 0000-0003-1217-537X
authors.initials: DJ
authors.lastname: Orr
authors.affiliation: Lancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, United Kingdom (D.J.O., A.A., E.C.-S., M.A.J.P.); Rothamsted Research, Plant Biology and Crop Science, Harpenden AL5 2JQ, United Kingdom (D.J.O., A.A., P.J.A., E.C.-S., M.A.J.P.); Plant Sciences Division, Research School of Biology, Australian National University, Canberra, ACT 0200, Australia (M.V.K.); and School of Natural Sciences and Psychology, Liverpool John Moores University, Liverpool L3 3AF, United Kingdom (M.V.K.).
authors.forename: André
authors.identifier: 0000-0003-2340-5927
authors.initials: A
authors.lastname: Alcântara
authors.affiliation: Lancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, United Kingdom (D.J.O., A.A., E.C.-S., M.A.J.P.); Rothamsted Research, Plant Biology and Crop Science, Harpenden AL5 2JQ, United Kingdom (D.J.O., A.A., P.J.A., E.C.-S., M.A.J.P.); Plant Sciences Division, Research School of Biology, Australian National University, Canberra, ACT 0200, Australia (M.V.K.); and School of Natural Sciences and Psychology, Liverpool John Moores University, Liverpool L3 3AF, United Kingdom (M.V.K.).
authors.forename: Maxim V
authors.identifier: 0000-0001-7966-0295
authors.initials: MV
authors.lastname: Kapralov
authors.affiliation: Lancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, United Kingdom (D.J.O., A.A., E.C.-S., M.A.J.P.); Rothamsted Research, Plant Biology and Crop Science, Harpenden AL5 2JQ, United Kingdom (D.J.O., A.A., P.J.A., E.C.-S., M.A.J.P.); Plant Sciences Division, Research School of Biology, Australian National University, Canberra, ACT 0200, Australia (M.V.K.); and School of Natural Sciences and Psychology, Liverpool John Moores University, Liverpool L3 3AF, United Kingdom (M.V.K.).
authors.forename: P John
authors.initials: PJ
authors.lastname: Andralojc
authors.affiliation: Lancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, United Kingdom (D.J.O., A.A., E.C.-S., M.A.J.P.); Rothamsted Research, Plant Biology and Crop Science, Harpenden AL5 2JQ, United Kingdom (D.J.O., A.A., P.J.A., E.C.-S., M.A.J.P.); Plant Sciences Division, Research School of Biology, Australian National University, Canberra, ACT 0200, Australia (M.V.K.); and School of Natural Sciences and Psychology, Liverpool John Moores University, Liverpool L3 3AF, United Kingdom (M.V.K.).
authors.forename: Elizabete
authors.identifier: 0000-0001-6059-9359
authors.initials: E
authors.lastname: Carmo-Silva
authors.affiliation: Lancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, United Kingdom (D.J.O., A.A., E.C.-S., M.A.J.P.); Rothamsted Research, Plant Biology and Crop Science, Harpenden AL5 2JQ, United Kingdom (D.J.O., A.A., P.J.A., E.C.-S., M.A.J.P.); Plant Sciences Division, Research School of Biology, Australian National University, Canberra, ACT 0200, Australia (M.V.K.); and School of Natural Sciences and Psychology, Liverpool John Moores University, Liverpool L3 3AF, United Kingdom (M.V.K.).
authors.forename: Martin A J
authors.identifier: 0000-0002-4477-672X
authors.initials: MA
authors.lastname: Parry
mesh_terms: D055162:Biocatalysis; D018556:Crops, Agricultural / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D014644:Genetic Variation; D007700:Kinetics; D010788:Photosynthesis / Q000235:genetics*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013696:Temperature
chemical_list: D010940:Plant Proteins; C101625:RbcL protein, plastid; D012273:Ribulose-Bisphosphate Carboxylase