title: Properties of the feline tumour suppressor reduced expression in immortalized cells (REIC/Dkk-3).
abstract: Reduced expression in immortalized cells (REIC/Dkk-3), a member of the human Dickkopf (Dkk) family, is a growth suppressor in human and canine mammary tumours. Mammary gland tumours are common neoplasms with high malignancy in female cats. The purpose of this study was to clone the feline REIC/Dkk-3 homolog, investigate its expression in cell lines established from feline mammary gland tumours, and test its tumour suppressor function. Western blot analysis revealed that expression of the REIC/Dkk-3 protein was reduced in feline mammary carcinoma cell lines. Forced expression of REIC/Dkk-3 induced apoptosis in feline mammary tumour cell lines. These results demonstrate that REIC/Dkk-3 expression, which is downregulated in feline mammary tumour cell lines, results in the induction of apoptosis in these cells. Our findings suggest that feline REIC/Dkk-3 represents a potential molecular target for the development of therapies against feline mammary cancers.
authors.affiliation: School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ochiai
authors.affiliation: School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Oda
authors.affiliation: School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Shono
authors.affiliation: School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kato
authors.affiliation: School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Sugihara
authors.affiliation: School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Nakazawa
authors.affiliation: School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: D
authors.initials: D
authors.lastname: Azakami
authors.affiliation: Department of Veterinary Pathology, School of Veterinary Medicine, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Michishita
authors.affiliation: School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Onozawa
authors.affiliation: Department of Veterinary Clinical Pathology, School of Veterinary Medicine, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Bonkobara
authors.affiliation: School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sako
authors.affiliation: Department of Urology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.
authors.forename: L
authors.initials: L
authors.lastname: Shun-Ai
authors.affiliation: Department of Urology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ueki
authors.affiliation: Department of Urology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Watanabe
authors.affiliation: School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Omi
mesh_terms: D000818:Animals; D017209:Apoptosis; D002371:Cat Diseases / Q000378:metabolism; D002415:Cats; D045744:Cell Line, Tumor / Q000378:metabolism; D003001:Cloning, Molecular; D005260:Female; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D015674:Mammary Neoplasms, Animal / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000378:metabolism*
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D025521:Tumor Suppressor Proteins
keywords: REIC/Dkk-3; cat; feline; mammary gland tumours; tumour suppressor gene