title: Piezo2 protein: A novel regulator of tumor angiogenesis and hyperpermeability.
abstract: Angiogenesis is important for invasive tumor growth and metastasis. Its inhibition is a promising tactic for limiting tumor progression. Here, we showed that Piezo2 knockdown led to decreased glioma angiogenesis and reduced vascular hyperpermeability. Piezo2 was highly expressed in tumor endothelial cells, and its knockdown suppressed vascular leakage and tumor angiogenesis. In a retinal vasculature development assay, corneal angiogenesis assay and a modified Miles assay, Piezo2 knockdown obviously decreased angiogenesis and vascular hyperpermeability. In vitro assays revealed that Piezo2 knockdown inhibited endothelial cell proliferation, migration, and tube formation. Moreover, In vitro co-culture system assay showed that Piezo2 knockdown in endothelial cells suppressed cell proliferation, migration, and invasion of glioma tumor cells. Piezo2 could regulate glioma angiogenesis via Ca2+/Wnt11/β-catenin signaling in endothelial cells. Taken together, these studies provide the evidence for Piezo2 as a critical regulator of tumor angiogenesis and vascular permeability.
authors.affiliation: Eye Hospital, Nanjing Medical University, Nanjing, China.|The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.affiliation: Eye Hospital, Nanjing Medical University, Nanjing, China.|The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing, China.
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.affiliation: Eye Hospital, Nanjing Medical University, Nanjing, China.|The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing, China.
authors.forename: Rong-Mei
authors.initials: RM
authors.lastname: Zhou
authors.affiliation: Eye Hospital, Nanjing Medical University, Nanjing, China.|The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Yao
authors.affiliation: Eye Hospital, Nanjing Medical University, Nanjing, China.
authors.forename: Xiu-Miao
authors.initials: XM
authors.lastname: Li
authors.affiliation: Eye Hospital, Nanjing Medical University, Nanjing, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Department of Neurology, Jiangsu Province Hospital, Nanjing, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Cheng
authors.affiliation: Department of Neurology, Jiangsu Chinese Medicine Hospital, Nanjing, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Yuan
authors.affiliation: The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing, China.
authors.forename: Biao
authors.initials: B
authors.lastname: Yan
authors.affiliation: Eye Hospital, Nanjing Medical University, Nanjing, China.|The Fourth School of Clinical Medicine, Nanjing Medical University, Nanjing, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Jiang
mesh_terms: D000818:Animals; D002199:Capillary Permeability / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D002478:Cells, Cultured; D005260:Female; D005910:Glioma / Q000098:blood supply / Q000235:genetics* / Q000628:therapy; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D007473:Ion Channels / Q000235:genetics* / Q000378:metabolism; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D009389:Neovascularization, Pathologic / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D000067492:RNAi Therapeutics
chemical_list: D007473:Ion Channels; C000589132:PIEZO2 protein, human; C552921:Piezo2 protein, mouse
keywords: Piezo2; endothelial cell; tumor angiogenesis; vascular permeability