title: Flow-cytometric analysis of reactive oxygen species in cancer cells under treatment with brassinosteroids.
abstract: To explore the underlying mechanism of cancer cell growth inhibition by brassinosteroids (BS), reactive oxygen species (ROS) generation under treatment with 28-homocastasterone and its synthetic derivatives (22S,23S)-28-homocastasterone was measured in A549 human lung adenocarcinoma cells. BS induced ROS generation in A549 cells and their growth in a time and dose-dependent manner. The maximal effect was observed for (22S,23S)-28-homocastasterone which at 30μM concentration showed a 6-fold increase of ROS generation in comparison with the control.
authors.affiliation: Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Kuprevich str., 5/2, 220141 Minsk, Belarus.
authors.forename: Pyotr A
authors.initials: PA
authors.lastname: Kisselev
authors.affiliation: Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Kuprevich str., 5/2, 220141 Minsk, Belarus.
authors.forename: Olesya V
authors.initials: OV
authors.lastname: Panibrat
authors.affiliation: Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Kuprevich str., 5/2, 220141 Minsk, Belarus.
authors.forename: Aliaksei R
authors.initials: AR
authors.lastname: Sysa
authors.affiliation: Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Kuprevich str., 5/2, 220141 Minsk, Belarus.
authors.forename: Marina V
authors.initials: MV
authors.lastname: Anisovich
authors.affiliation: Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Kuprevich str., 5/2, 220141 Minsk, Belarus. Electronic address: vz@iboch.bas-net.by.
authors.forename: Vladimir N
authors.initials: VN
authors.lastname: Zhabinskii
authors.affiliation: Institute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Kuprevich str., 5/2, 220141 Minsk, Belarus.
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Khripach
mesh_terms: D000072283:A549 Cells; D000970:Antineoplastic Agents / Q000494:pharmacology*; D060406:Brassinosteroids / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects*; D005434:Flow Cytometry; D006801:Humans; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D060406:Brassinosteroids; D017382:Reactive Oxygen Species
keywords: 28-Homocastasterone; Anticancer; Brassinosteroids; Cancer cell line A549; Reactive oxygen species