title: Characterization of rhizosphere and endophytic fungal communities from roots of Stipa purpurea in alpine steppe around Qinghai Lake.
abstract: Stipa purpurea is among constructive endemic species in the alpine steppe on the Qinghai-Xizang Plateau. To reveal the fungal community structure and diversity in the rhizosphere and roots of this important grass and to analyze the potential influence of different habitats on the structure of fungal communities, we explored the root endophyte and the directly associated rhizosphere communities of S. purpurea by using internal transcribed spacer rRNA cloning and sequencing methods. We found that the roots of S. purpurea are associated with a diverse consortium of Basidiomycota (59.8%) and Ascomycota (38.5%). Most fungi obtained from rhizosphere soil in S. purpurea have been identified as Ascomycetes, while the high proportion detected in roots were basidiomycetous endophytes. The species richness, diversity, and evenness of fungal assemblages were higher in roots than in the rhizosphere soil. Fungi inhabiting the rhizosphere and roots of S. purpurea are significantly different, and the rhizosphere and endophyte communities are largely independent with little overlap in the dominant phyla or operational taxonomic units. Taken together, these results suggested that a wide variety of fungal communities are associated with the roots and rhizosphere soil of S. purpurea and that the fungal assemblages are strongly influenced by different habitats.
authors.affiliation: a Institute of Biology, Gansu Academy of Sciences, Lanzhou 730000, People's Republic of China.
authors.forename: Dengxue
authors.initials: D
authors.lastname: Lu
authors.affiliation: b Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Jin
authors.affiliation: b Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, People's Republic of China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Yang
authors.affiliation: c College of Grassland Science, Key Laboratory of Grassland Ecosystem (Ministry of Education), Pratacultural Engineering Laboratory of Gansu Province, Sino-U.S. Centers for Grazing land Ecosystem Sustainability, Gansu Agricultural University, Lanzhou 730070, People's Republic of China.
authors.forename: Denghong
authors.initials: D
authors.lastname: Zhang
authors.affiliation: b Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, People's Republic of China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Yan
authors.affiliation: b Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, People's Republic of China.
authors.forename: Xiuzhuang
authors.initials: X
authors.lastname: Li
authors.affiliation: a Institute of Biology, Gansu Academy of Sciences, Lanzhou 730000, People's Republic of China.
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: a Institute of Biology, Gansu Academy of Sciences, Lanzhou 730000, People's Republic of China.
authors.forename: Rongbing
authors.initials: R
authors.lastname: Han
authors.affiliation: b Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Qin
mesh_terms: D001203:Ascomycota / Q000235:genetics / Q000302:isolation & purification*; D001487:Basidiomycota / Q000235:genetics / Q000302:isolation & purification*; D060026:Endophytes / Q000235:genetics / Q000302:isolation & purification*; D060106:Lakes; D018517:Plant Roots / Q000382:microbiology; D006109:Poaceae / Q000382:microbiology*; D058441:Rhizosphere; D012987:Soil; D012988:Soil Microbiology
chemical_list: D012987:Soil
keywords: ARNr ITS; ITS rRNA; Stipa purpurea; alpine steppe; analyse phylogénique; endophyte de graminée; grass endophyte; phylogenic analysis; steppe alpine