title: Functionalization of Carbon Nanomaterial Surface by Doxorubicin and Antibodies to Tumor Markers.
abstract: The actual task of oncology is effective treatment of cancer while causing a minimum harm to the patient. The appearance of polymer nanomaterials and technologies launched new applications and approaches of delivery and release of anticancer drugs. The goal of work was to test ultra dispersed diamonds (UDDs) and onion-like carbon (OLCs) as new vehicles for delivery of antitumor drug (doxorubicin (DOX)) and specific antibodies to tumor receptors. Stable compounds of UDDs and OLCs with DOX were obtained. As results of work, an effectiveness of functionalization was 2.94 % w/w for OLC-DOX and 2.98 % w/w for UDD-DOX. Also, there was demonstrated that UDD-DOX and OLC-DOX constructs had dose-dependent cytotoxic effect on tumor cells in the presence of trypsin. The survival of adenocarcinoma cells reduced from 52 to 28 % in case of incubation with the UDD-DOX in concentrations from 8.4-2.5 to 670-20 μg/ml and from 72 to 30 % after incubation with OLC-DOX. Simultaneously, antibodies to epidermal growth factor maintained 75 % of the functional activity and specificity after matrix-assisted pulsed laser evaporation deposition. Thus, the conclusion has been made about the prospects of selected new methods and approaches for creating an antitumor agent with capabilities targeted delivery of drugs.
authors.affiliation: Department of biotechnical problems of diagnostic IPCC, Nauky str.,42/1, Kiev, 03028, Ukraine.
authors.forename: Olena M
authors.initials: OM
authors.lastname: Perepelytsina
authors.affiliation: Department of biotechnical problems of diagnostic IPCC, Nauky str.,42/1, Kiev, 03028, Ukraine. olenaquail@rambler.ru.
authors.forename: Olena M
authors.initials: OM
authors.lastname: Yakymchuk
authors.affiliation: Department of biotechnical problems of diagnostic IPCC, Nauky str.,42/1, Kiev, 03028, Ukraine.
authors.forename: Mychailo V
authors.initials: MV
authors.lastname: Sydorenko
authors.affiliation: Chuiko Institute of Surface Chemistry NAS of Ukraine, 17 General Naumov str., Kiev, 03164, Ukraine.
authors.forename: Olga N
authors.initials: ON
authors.lastname: Bakalinska
authors.affiliation: Dipartimento di Fisica, Università degli studi di Napoli Federico II, Piazzale Teccnio, Naples, 80-80125, Italy.|SPIN-CNR, Via Cintia 1, Naples, 80124, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Bloisi
authors.affiliation: Dipartimento di Fisica, Università degli studi di Napoli Federico II, Piazzale Teccnio, Naples, 80-80125, Italy.|SPIN-CNR, Via Cintia 1, Naples, 80124, Italy.
authors.forename: Luciano Rosario Maria
authors.initials: LR
authors.lastname: Vicari
keywords: Doxorubicin; Functionalization; MAPLE deposition; MCF-7; Onion-like carbons; Ultra dispersed diamonds