title: Set-up and application of an analytical approach for the quality control of purified colostrum as food supplement.
abstract: A validated analytical procedure is here described for the quality control of the protein fraction of purified bovine colostrum used in food supplements. The proposed procedure starts with 1D and 2D-gel electrophoresis. The sample is then separated into two fractions by protein G affinity chromatography: the IgG enriched and the IgG depleted fraction (IgG-d). A size exclusion chromatography coupled to UV is then applied to the IgG and IgG-d fractions for the quantitative analysis of IgG and IgM, respectively. The IgG-d fraction is then analysed by HPLC-MS analysis for the quantitative analysis of β-lactoglobulins and α-lactoalbumin. The next step is to quantitatively measure a set of bioactive proteins selected from the bovine colostrum data bank on the basis of their claimed health benefits. The enzymatic activities of lactoperoxidase and xanthine dehydrogenase/oxidase are then tested as an index of protein functionality.
authors.affiliation: Department of Pharmaceutical Sciences, Università degli Studi di Milano, Via Mangiagalli 25, 20133 Milano, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Altomare
authors.affiliation: Department of Pharmaceutical Sciences, Università degli Studi di Milano, Via Mangiagalli 25, 20133 Milano, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Regazzoni
authors.affiliation: Department of Pharmaceutical Sciences, Università degli Studi di Milano, Via Mangiagalli 25, 20133 Milano, Italy.
authors.forename: Ximena Maria Paredes
authors.initials: XMP
authors.lastname: Parra
authors.affiliation: Department of Pharmaceutical Sciences, Università degli Studi di Milano, Via Mangiagalli 25, 20133 Milano, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Selmin
authors.affiliation: Department of Pharmacology and Biomolecular Sciences, Università degli Studi di Milano, Via Trentacoste 2, 20133 Milan, Italy.
authors.forename: Cristiano
authors.initials: C
authors.lastname: Rumio
authors.affiliation: Department of Pharmaceutical Sciences, Università degli Studi di Milano, Via Mangiagalli 25, 20133 Milano, Italy.
authors.forename: Marina
authors.initials: M
authors.lastname: Carini
authors.affiliation: Department of Pharmaceutical Sciences, Università degli Studi di Milano, Via Mangiagalli 25, 20133 Milano, Italy. Electronic address: giancarlo.aldini@unimi.it.
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Aldini
mesh_terms: D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003126:Colostrum / Q000737:chemistry* / Q000201:enzymology / Q000378:metabolism; D019587:Dietary Supplements / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional; D057075:Enzyme Assays; D005260:Female; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007784:Lactoperoxidase / Q000032:analysis / Q000378:metabolism; D013058:Mass Spectrometry; D011247:Pregnancy; D011786:Quality Control; D014968:Xanthine Dehydrogenase / Q000032:analysis / Q000378:metabolism
chemical_list: D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007784:Lactoperoxidase; D014968:Xanthine Dehydrogenase
keywords: Bovine colostrum; Food supplement; Mass spectrometry; Quality control; Size exclusion chromatography