title: Paralleled comparison of vectors for the generation of CAR-T cells.
abstract: T-lymphocytes genetically engineered with the chimeric antigen receptor (CAR-T) have shown great therapeutic potential in cancer treatment. A variety of preclinical researches and clinical trials of CAR-T therapy have been carried out to lay the foundation for future clinical application. In these researches, several gene-transfer methods were used to deliver CARs or other genes into T-lymphocytes, equipping CAR-modified T cells with a property of recognizing and attacking antigen-expressing tumor cells in a major histocompatibility complex-independent manner. Here, we summarize the gene-transfer vectors commonly used in the generation of CAR-T cell, including retrovirus vectors, lentivirus vectors, the transposon/transposase system, the plasmid-based system, and the messenger RNA electroporation system. The following aspects were compared in parallel: efficiency of gene transfer, the integration methods in the modified T cells, foreground of scale-up production, and application and development in clinical trials. These aspects should be taken into account to generate the optimal CAR-gene vector that may be suitable for future clinical application.
authors.affiliation: West China Medical School, State Key Laboratory of Biotherapy/Collaborative Innovation Center for Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, China.
authors.forename: Di-Yuan
authors.initials: DY
authors.lastname: Qin
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Yong-Sheng
authors.initials: YS
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Yu-Quan
authors.initials: YQ
authors.lastname: Wei
mesh_terms: D000818:Animals; D002986:Clinical Trials as Topic; D004251:DNA Transposable Elements; D018274:Electroporation / Q000379:methods; D005822:Genetic Vectors; D006801:Humans; D007167:Immunotherapy / Q000379:methods; D011948:Receptors, Antigen, T-Cell / Q000235:genetics* / Q000378:metabolism; D013601:T-Lymphocytes / Q000502:physiology*; D014161:Transduction, Genetic / Q000379:methods*; D014162:Transfection / Q000379:methods*
chemical_list: D004251:DNA Transposable Elements; D011948:Receptors, Antigen, T-Cell