title: Correlation between IL-6 and invasiveness of ectoderm cells of embryo in early pregnancy.
abstract: This study aimed to explore the correlation between Interleukin-6 (IL-6) and invasiveness of ectoderm cells of embryo in early pregnancy, in order to further discuss whether IL-6 can enhance invasiveness of ectoderm cells. The study lays the foundation for determination of pathogenesis of some gestation period-related diseases. Differences in mRNA and protein expression of trophoblastic cell line JEG-3 cells in IL-6, matrix metalloproteinase-2 (MMP-2) and MMP-9 were analyzed; the regulating effect of different concentrations of IL-6 on invasive ability of trophoblast cells was studied by Transwell assay; the effect of IL-6 on proliferation of ectodermal cell line JEG-3 of embryo was analyzed by methyl thiazolyl tetrazolium (MTT) assay. The invasive number of JEG-3 cells incubated by IL-6 (10 ng/ml) was higher than that of the control group, and the difference had statistical significance (p < 0.05). Results of using MMT assay to detect the effect of IL-6 on proliferation of trophoblastic cell line JEG-3 showed that JEG-3 cells before and after processing had no significant difference from the control group (p >0.05). Therefore, IL-6 can enhance invasiveness of ectoderm cells of embryo through activation of MMP-2.
authors.affiliation: Gynecology and Obstetrics, Peoples Hospital of Liaocheng, Shandong, China.
authors.forename: X Y
authors.initials: XY
authors.lastname: Jiang
authors.affiliation: Gynecology and Obstetrics, Peoples Hospital of Liaocheng, Shandong, China.
authors.forename: T M
authors.initials: TM
authors.lastname: Lu
authors.affiliation: Teaching and Research Office of Medical Science, Nursing Academy, Binzhou Polytechnic, Shandong, China.
authors.forename: W H
authors.initials: WH
authors.lastname: Shu
authors.affiliation: Gynecology and Obstetrics, Peoples Hospital of Liaocheng, Shandong, China.
authors.forename: H Y
authors.initials: HY
authors.lastname: Zhou
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D004475:Ectoderm / Q000187:drug effects* / Q000201:enzymology; D005260:Female; D006801:Humans; D015850:Interleukin-6 / Q000494:pharmacology*; D020778:Matrix Metalloproteinase 2 / Q000235:genetics; D020780:Matrix Metalloproteinase 9 / Q000235:genetics; D011247:Pregnancy
chemical_list: D015850:Interleukin-6; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9