title: Recombinant Sheep Pox Virus Proteins Elicit Neutralizing Antibodies.
abstract: The aim of this work was to evaluate the immunogenicity and neutralizing activity of sheep pox virus (SPPV; genus Capripoxvirus, family Poxviridae) structural proteins as candidate subunit vaccines to control sheep pox disease. SPPV structural proteins were identified by sequence homology with proteins of vaccinia virus (VACV) strain Copenhagen. Four SPPV proteins (SPPV-ORF 060, SPPV-ORF 095, SPPV-ORF 117, and SPPV-ORF 122), orthologs of immunodominant L1, A4, A27, and A33 VACV proteins, respectively, were produced in Escherichia coli. Western blot analysis revealed the antigenic and immunogenic properties of SPPV-060, SPPV-095, SPPV-117 and SPPV-122 proteins when injected with adjuvant into experimental rabbits. Virus-neutralizing activity against SPPV in lamb kidney cell culture was detected for polyclonal antisera raised to SPPV-060, SPPV-117, and SPPV-122 proteins. To our knowledge, this is the first report demonstrating the virus-neutralizing activities of antisera raised to SPPV-060, SPPV-117, and SPPV-122 proteins.
authors.affiliation: Research Institute for Biological Safety Problems, RK ME&amp;S - Science Committee, Gvardeiskiy 080409, Kazakhstan. ovch@mail.ru.
authors.forename: Olga V
authors.initials: OV
authors.lastname: Chervyakova
authors.affiliation: Research Institute for Biological Safety Problems, RK ME&amp;S - Science Committee, Gvardeiskiy 080409, Kazakhstan. biosafety@biosafety.kz.
authors.forename: Valentin L
authors.initials: VL
authors.lastname: Zaitsev
authors.affiliation: M. A. Aitkhozhin's Institute of Molecular Biology and Biochemistry, RK ME&amp;S - Science Committee, Almaty 050012, Kazakhstan. bulat.isakakov@mail.ru.
authors.forename: Bulat K
authors.initials: BK
authors.lastname: Iskakov
authors.affiliation: Research Institute for Biological Safety Problems, RK ME&amp;S - Science Committee, Gvardeiskiy 080409, Kazakhstan. tailakova_86@mail.ru.
authors.forename: Elmira T
authors.initials: ET
authors.lastname: Tailakova
authors.affiliation: Research Institute for Biological Safety Problems, RK ME&amp;S - Science Committee, Gvardeiskiy 080409, Kazakhstan. vstrochkov@biosafety.kz.
authors.forename: Vitaliy M
authors.initials: VM
authors.lastname: Strochkov
authors.affiliation: Research Institute for Biological Safety Problems, RK ME&amp;S - Science Committee, Gvardeiskiy 080409, Kazakhstan. sultankul70@mail.ru.
authors.forename: Kulyaisan T
authors.initials: KT
authors.lastname: Sultankulova
authors.affiliation: Research Institute for Biological Safety Problems, RK ME&amp;S - Science Committee, Gvardeiskiy 080409, Kazakhstan. nurlan.s@mail.ru.
authors.forename: Nurlan T
authors.initials: NT
authors.lastname: Sandybayev
authors.affiliation: M. A. Aitkhozhin's Institute of Molecular Biology and Biochemistry, RK ME&amp;S - Science Committee, Almaty 050012, Kazakhstan. gulshanst@yahoo.com.
authors.forename: Gulshan E
authors.initials: GE
authors.lastname: Stanbekova
authors.affiliation: M. A. Aitkhozhin's Institute of Molecular Biology and Biochemistry, RK ME&amp;S - Science Committee, Almaty 050012, Kazakhstan. daniyar.b@mail.ru.
authors.forename: Daniyar K
authors.initials: DK
authors.lastname: Beisenov
authors.affiliation: Research Institute for Biological Safety Problems, RK ME&amp;S - Science Committee, Gvardeiskiy 080409, Kazakhstan. yergali.a@gmail.com.
authors.forename: Yergali O
authors.initials: YO
authors.lastname: Abduraimov
authors.affiliation: Research Institute for Biological Safety Problems, RK ME&amp;S - Science Committee, Gvardeiskiy 080409, Kazakhstan. murat@biosafety.kz.
authors.forename: Muratbay
authors.initials: M
authors.lastname: Mambetaliyev
authors.affiliation: Research Institute for Biological Safety Problems, RK ME&amp;S - Science Committee, Gvardeiskiy 080409, Kazakhstan. sansyzbai-ar@biosafety.kz.
authors.forename: Abylay R
authors.initials: AR
authors.lastname: Sansyzbay
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, Molecular Plant Pathology Laboratory, Beltsville, MD 20705, USA. natalia.kovalksaya@ars.usda.gov.
authors.forename: Natalia Y
authors.initials: NY
authors.lastname: Kovalskaya
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, Molecular Plant Pathology Laboratory, Beltsville, MD 20705, USA. lev.nemchinov@ars.usda.gov.
authors.forename: Lev G
authors.initials: LG
authors.lastname: Nemchinov
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, Molecular Plant Pathology Laboratory, Beltsville, MD 20705, USA. rose.hammond@ars.usda.gov.
authors.forename: Rosemarie W
authors.initials: RW
authors.lastname: Hammond
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood*; D000914:Antibodies, Viral / Q000097:blood*; D015153:Blotting, Western; D018151:Capripoxvirus / Q000235:genetics / Q000276:immunology*; D002460:Cell Line; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D009500:Neutralization Tests; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology*; D012756:Sheep; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000276:immunology; D010948:Viral Plaque Assay; D015678:Viral Structural Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D011994:Recombinant Proteins; D022223:Vaccines, Subunit; D015678:Viral Structural Proteins; D014765:Viral Vaccines
keywords: immunogenicity; neutralizing antibodies; prokaryotic expression; recombinant protein; sheep pox virus; vaccinia virus