title: Optimization of L-asparaginase production from novel Enterobacter sp., by submerged fermentation using response surface methodology.
abstract: The culture conditions and nutritional rations influencing the production of extra cellular antileukemic enzyme by novel Enterobacter aerogenes KCTC2190/MTCC111 were optimized in shake-flask culture. Process variables like pH, temperature, incubation time, carbon and nitrogen sources, inducer concentration, and inoculum size were taken into account. In the present study, finest enzyme activity achieved by traditional one variable at a time method was 7.6 IU/mL which was a 2.6-fold increase compared to the initial value. Further, the L-asparaginase production was optimized using response surface methodology, and validated experimental result at optimized process variables gave 18.35 IU/mL of L-asparaginase activity, which is 2.4-times higher than the traditional optimization approach. The study explored the E. aerogenes MTCC111 as a potent and potential bacterial source for high yield of antileukemic drug.
authors.affiliation: a Department of Biotechnology, National Institute of Technology Warangal , Warangal , Telangana , India.
authors.forename: Rajeswara Reddy
authors.initials: RR
authors.lastname: Erva
authors.affiliation: a Department of Biotechnology, National Institute of Technology Warangal , Warangal , Telangana , India.
authors.forename: Ajgebi Nath
authors.initials: AN
authors.lastname: Goswami
authors.affiliation: a Department of Biotechnology, National Institute of Technology Warangal , Warangal , Telangana , India.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Suman
authors.affiliation: a Department of Biotechnology, National Institute of Technology Warangal , Warangal , Telangana , India.
authors.forename: Ravali
authors.initials: R
authors.lastname: Vedanabhatla
authors.affiliation: a Department of Biotechnology, National Institute of Technology Warangal , Warangal , Telangana , India.
authors.forename: Satish Babu
authors.initials: SB
authors.lastname: Rajulapati
mesh_terms: D000970:Antineoplastic Agents / Q000378:metabolism*; D001215:Asparaginase / Q000378:metabolism*; D001216:Asparagine / Q000378:metabolism; D061249:Batch Cell Culture Techniques / Q000379:methods*; D002244:Carbon / Q000378:metabolism; D021902:Enterobacter aerogenes / Q000201:enzymology* / Q000378:metabolism; D005285:Fermentation; D007218:Industrial Microbiology / Q000379:methods*; D009584:Nitrogen / Q000378:metabolism; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D000970:Antineoplastic Agents; D001216:Asparagine; D002244:Carbon; D001215:Asparaginase; D009584:Nitrogen
keywords: Enterobacter aerogenes MTCC111; L-Asparaginase; antileukemic drug; enzyme activity; optimization; substrate specificity