title: A new approach of microalgal biomass pretreatment using deep eutectic solvents for enhanced lipid recovery for biodiesel production.
abstract: The biomass of Chlorella sp. was pretreated with three different aqueous deep eutectic solvents (aDESs), i.e. aqueous choline chloride-oxalic acid (aCh-O), aqueous choline chloride-ethylene glycol (aCh-EG) and aqueous urea-acetamide (aU-A). The effect of aDESs pretreatment of microalgae biomass was evaluated in terms of lipid recovery rate, total carbohydrate content, fatty acid composition, and thermal chemical behavior of biomass. Results indicated that, lipid recovery rate was increased from 52.03% of untreated biomass to 80.90%, 66.92%, and 75.26% of the biomass treated by aCh-O, aCh-EG and aU-A, respectively. However, there were no major changes observed in fatty acid profiles of both untreated and treated biomass, specifically palmitic acid, palmitoleic acid and stearic acid under various pretreatments. Furthermore, characterizations of untreated and treated biomass were carried out using Fourier transform infrared (FTIR), thermogravimetry analysis (TGA) and scanning electron microscope (SEM) to understand the enhanced lipids recovery.
authors.affiliation: School of Chemistry and Environmental Engineering, Shaoguan University, Shaoguan 512005, China; Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Lu
authors.affiliation: Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China.
authors.forename: Md Asraful
authors.initials: MA
authors.lastname: Alam
authors.affiliation: Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China; Nano Science and Technology Institute, University of Science and Technology China, Suzhou 215123, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Jingcheng
authors.initials: J
authors.lastname: Wu
authors.affiliation: Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China. Electronic address: wangzm@ms.giec.ac.cn.
authors.forename: Zhongming
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China.
authors.forename: Zhenhong
authors.initials: Z
authors.lastname: Yuan
mesh_terms: D000081:Acetamides / Q000737:chemistry; D056804:Biofuels; D018533:Biomass; D002708:Chlorella / Q000737:chemistry*; D002794:Choline / Q000737:chemistry; D019855:Ethylene Glycol / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D008055:Lipids / Q000737:chemistry*; D058086:Microalgae / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D019815:Oxalic Acid / Q000737:chemistry; D019308:Palmitic Acid / Q000737:chemistry; D012997:Solvents / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry; D014508:Urea / Q000737:chemistry
chemical_list: D000081:Acetamides; D056804:Biofuels; D005227:Fatty Acids; D008055:Lipids; D012997:Solvents; D019308:Palmitic Acid; D014508:Urea; C030686:acetamide; D019815:Oxalic Acid; D019855:Ethylene Glycol; D002794:Choline
keywords: Biodiesel; Deep eutectic solvents; Lipid extraction; Microalgae biomass; Pretreatment