title: Evaluation of salivary DNA obtained from dental prosthesis and its applicability in forensic investigations.
abstract: OBJECTIVE
  This study evaluated the salivary DNA obtained from dental prosthesis after a period of storage and its applicability in human identification.
  
  
  METHODS
  In first phase, DNA was extracted after a span of 1 week and 1 month from the dental prosthesis dipped in whole saliva for 15 min. It was followed by PCR and electrophoresis. In second phase, from extracted DNA samples 15 STRs (short tandem repeats) of human genomic DNA were amplified via polymerase chain reaction.
  
  
  CONCLUSIONS
  DNA isolated from saliva stained dental prosthesis after a period of storage and the techniques employed are adequate for further forensic analysis.
authors.affiliation: Oral Pathology and Microbiology, Navi Mumbai, Maharashtra, India. Electronic address: ish30@yahoo.com.
authors.forename: Isha
authors.initials: I
authors.lastname: Chauhan
authors.affiliation: Oral Pathology and Microbiology, SRM Dental College, Ramapuram, Chennai, India.
authors.forename: G
authors.initials: G
authors.lastname: Sugirtharaj
authors.affiliation: Human Molecular Genetics Lab, Rajiv Gandhi Centre for Biotechnology, Trivandrum, Kerala, India.
authors.forename: Sanish
authors.initials: S
authors.lastname: Sathyan
authors.affiliation: Oral Pathology and Microbiology, GDC, Kottayam, Kerala, India.
authors.forename: V T
authors.initials: VT
authors.lastname: Beena
authors.affiliation: Human Molecular Genetics Lab, Rajiv Gandhi Centre for Biotechnology, Trivandrum, Kerala, India.
authors.forename: Moinak
authors.initials: M
authors.lastname: Banerjee
mesh_terms: D004247:DNA / Q000032:analysis*; D016172:DNA Fingerprinting / Q000379:methods*; D017266:Dental Prosthesis; D004586:Electrophoresis; D006801:Humans; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D012463:Saliva / Q000737:chemistry*
chemical_list: D004247:DNA
keywords: DNA; Dental prosthesis; STRs; Saliva