title: Fabrication of Apigenin loaded gellan gum-chitosan hydrogels (GGCH-HGs) for effective diabetic wound healing.
abstract: The Apigenin (APN) was isolated from ethanolic extract of M. alba leaves and screened by in-vivo wound models (Diabetic and Dead space) in rats. Apigenin loaded hydrogel (HGs) was prepared using gellan gum-chitosan (GGCH) with PEG as a cross linker and characterized for various parameter like AFM, swelling property, entrapment efficiency and drug release. Further performance of hydrogel was evaluated by wound healing activity tested against wound contraction, collagen content, dried granuloma weights and antioxidant activity. The percent entrapment efficiency of optimized hydrogel found to be 87.15±1.20. APN loaded GGCH-HGs were able to release 96.11% APN in 24h. The level of superoxide dismutase (SOD) and catalase were found increased significantly in granuloma tissue of APN treated group. APN GGCH-HGs found higher wound healing effect in diabetic as well as normal wound tissues with significant antioxidant activity. Results proven the utility of prepared hydrogel (APN loaded GGCH-HGs) seems to be highly suitable for wound healing due to its unique properties of biocompatibility, biodegradability, moist nature and antioxidant effectiveness.
authors.affiliation: Department of Pharmaceutical Science, Suresh Gyan Vihar University, Jaipur, RJ, India.
authors.forename: Rajesh
authors.initials: R
authors.lastname: Shukla
authors.affiliation: Department of Pharmaceutical Sciences, Dr. H. S. Gour Central University, Sagar, MP, 470003, India. Electronic address: sushilkkashaw@gmail.com.
authors.forename: Sushil K
authors.initials: SK
authors.lastname: Kashaw
authors.affiliation: Department of Pharmacy, RKDF College of Pharmacy, SRK University, Bhopal, MP, India.
authors.forename: Alok Pal
authors.initials: AP
authors.lastname: Jain
authors.affiliation: Department of Pharmacy, Guru Ramdas Khalsa Institute of Science & Technology, Jabalpur, MP, India.
authors.forename: Santram
authors.initials: S
authors.lastname: Lodhi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D047310:Apigenin / Q000737:chemistry / Q000494:pharmacology*; D048271:Chitosan / Q000737:chemistry*; D003094:Collagen / Q000378:metabolism; D003920:Diabetes Mellitus / Q000503:physiopathology*; D004337:Drug Carriers / Q000737:chemistry; D065546:Drug Liberation; D005260:Female; D020100:Hydrogels / Q000737:chemistry*; D008297:Male; D018515:Plant Leaves / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000187:drug effects / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000975:Antioxidants; D004337:Drug Carriers; D020100:Hydrogels; D011135:Polysaccharides, Bacterial; C048288:gellan gum; D047310:Apigenin; D003094:Collagen; D048271:Chitosan
keywords: Antioxidant; Apigenin; Hydrogels; Morus alba; Wound healing