title: Serum expression levels of microRNA-382-3p, -598-3p, -1246 and -184 in breast cancer patients.
abstract: The purpose of the present study was to investigate the serum levels of microRNA (miRNA/miR)-382-3p, -598-3p, -1246 and -184 in breast cancer patients and to assess their feasibility as biomarkers for breast cancer screening. Serum samples were obtained from 100 breast cancer patients and 40 age-matched healthy control subjects in Taizhou Central Hospital (Taizhou, Zhejiang, China) between January 2013 and September 2014. The serum expression levels of miR-382-3p, -598-3p, -1246 and -184 were determined by stem-loop reverse transcription-quantitative polymerase chain reaction. Receiver operating characteristic curves were drawn to evaluate the sensitivity and specificity of the serum miRNA expression levels for the screening of breast cancer. miR-382-3p and -1246 were significantly upregulated in the serum of the breast cancer patients, while miR-598-3p and -184 were significantly downregulated. The sensitivity and specificity to detect breast cancer were as follows: miR-382-3p, 52.0 and 92.5%; miR-598-3p, 95.0 and 85.0%; miR-1246, 93.0 and 75.0%; and miR-184, 87.5 and 71.0%, respectively. The expression levels of the four serum miRNAs were not correlated with the patients' clinical stage. In summary, miR-382-3p, -598-3p, -1246 and -184 are all involved in the development of breast cancer, and are promising biomarkers for breast cancer detection.
authors.affiliation: College of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China.
authors.forename: Lun
authors.initials: L
authors.lastname: Fu
authors.affiliation: College of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China; Taizhou Central Hospital, Taizhou, Zhejiang 318000, P.R. China.
authors.forename: Zhaoyun
authors.initials: Z
authors.lastname: Li
authors.affiliation: Taizhou Central Hospital, Taizhou, Zhejiang 318000, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Taizhou Central Hospital, Taizhou, Zhejiang 318000, P.R. China.
authors.forename: Pan
authors.initials: P
authors.lastname: Wang
authors.affiliation: Taizhou Central Hospital, Taizhou, Zhejiang 318000, P.R. China.
authors.forename: Guangmin
authors.initials: G
authors.lastname: Fan
authors.affiliation: Taizhou Central Hospital, Taizhou, Zhejiang 318000, P.R. China.
authors.forename: Yuechu
authors.initials: Y
authors.lastname: Dai
authors.affiliation: Taizhou Central Hospital, Taizhou, Zhejiang 318000, P.R. China.
authors.forename: Zhibao
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, P.R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
keywords: biomarker; breast cancer; microRNAs; quantitative polymerase chain reaction