title: Fast activated charcoal prepurification of Fusarium solani β-glucosidase for an efficient oleuropein bioconversion.
abstract: Fungal β-glucosidases were extensively studied regarding their various potential biotechnology applications. Here, we report the selection of Fusarium solani strain producing high yield of β-glucosidase activity. The effect of some factors on β-glucosidase production was studied including: Initial pH, medium composition, concentration of carbon and nitrogen sources, and particle size of raw substrates. The optimal enzyme production was obtained with 4 units of pH. The highest β-glucosidase activity was produced on 4% wheat bran (WB) as raw carbon sources, reaching 5 U/mL. A positive correlation between WB particle size and the β-glucosidase production level was settled. The last one was enhanced to 13.60 U/mL in the presence of 0.5% (w/v) of ammonium sulfate. Interestingly, the activated charcoal was used as an inexpensive reagent enabling a rapid and efficient purification prior step that improved the enzyme-specific activity. Eventually, F. solani β-glucosidase acts efficiently during the bioconversion process of oleuropein. Indeed, 82.5% of oleuropein was deglycosylated after 1 hr at 40°C. Altogether, our data showed that the β-glucosidase of F. solani has a potential application to convert oleuropein to ameliorate food quality.
authors.affiliation: a Laboratory of Molecular Biotechnology of Eucaryotes , Centre of Biotechnology of Sfax (CBS), University of Sfax , Sfax , Tunisia.
authors.forename: Manel
authors.initials: M
authors.lastname: Boudabbous
authors.affiliation: b Biotechnology and Plant Improvement Laboratory , Centre of Biotechnology of Sfax (CBS), University of Sfax , Sfax , Tunisia.
authors.forename: Walid
authors.initials: W
authors.lastname: Saibi
authors.affiliation: c Environmental Bioprocesses Laboratory , Centre of Biotechnology of Sfax (CBS), University of Sfax , Sfax , Tunisia.
authors.forename: Zouhaier
authors.initials: Z
authors.lastname: Bouallagui
authors.affiliation: a Laboratory of Molecular Biotechnology of Eucaryotes , Centre of Biotechnology of Sfax (CBS), University of Sfax , Sfax , Tunisia.
authors.forename: Mosbeh
authors.initials: M
authors.lastname: Dardouri
authors.affiliation: c Environmental Bioprocesses Laboratory , Centre of Biotechnology of Sfax (CBS), University of Sfax , Sfax , Tunisia.
authors.forename: Sami
authors.initials: S
authors.lastname: Sayadi
authors.affiliation: a Laboratory of Molecular Biotechnology of Eucaryotes , Centre of Biotechnology of Sfax (CBS), University of Sfax , Sfax , Tunisia.
authors.forename: Hafedh
authors.initials: H
authors.lastname: Belghith
authors.affiliation: d Laboratory of Enzyme Engineering and Microbiology, ENIS , University of Sfax , Sfax , Tunisia.
authors.forename: Tahar
authors.initials: T
authors.lastname: Mechichi
authors.affiliation: a Laboratory of Molecular Biotechnology of Eucaryotes , Centre of Biotechnology of Sfax (CBS), University of Sfax , Sfax , Tunisia.
authors.forename: Ali
authors.initials: A
authors.lastname: Gargouri
mesh_terms: D002606:Charcoal / Q000737:chemistry*; D003470:Culture Media; D005670:Fusarium / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D057888:Iridoid Glucosides; D039823:Iridoids / Q000378:metabolism*; D001617:beta-Glucosidase / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D003470:Culture Media; D057888:Iridoid Glucosides; D039823:Iridoids; D002606:Charcoal; C002769:oleuropein; D001617:beta-Glucosidase
keywords: Activated charcoal; bioconversion; oleuropein; prepurification; wheat bran; β-glucosidase