title: Nanostructured SBA-15 silica: An effective protective vehicle to oral hepatitis B vaccine immunization.
abstract: Due to its physicochemical properties, nanostructured mesoporous SBA-15 silica shows great potential as a vaccine adjuvant. This study evaluated the capacity of SBA-15 to encapsulate/adsorb the recombinant purified HBsAg from the Hepatitis B virus and the immunoresponsiveness of mice orally immunized with HBsAg inside SBA-15. A simulation of small angle X-ray scattering experimental results, together with the nitrogen adsorption isotherms data, allowed to determine the appropriate mass ratio of HBsAg:SBA-15, indicating antigen encapsulation into SBA-15 macroporosity. This was also evaluated by bicinchoninic acid assay and gel electrophoresis. The recruitment of inflammatory cells, an increase in production of specific antibodies, and the non-influence of silica on TH1 or TH2 polarization were observed after oral immunization. Besides, SBA-15 enhanced the phagocytosis of ovalbumin by dendritic cells, an important key to prove how this adjuvant works. Thus, it seems clear that the nanostructured SBA-15 is an effective and safe adjuvant for oral immunizations.
authors.affiliation: Immunochemistry Laboratory, Butantan Institute, São Paulo, Brazil.
authors.forename: Karina
authors.initials: K
authors.lastname: Scaramuzzi
authors.affiliation: Immunochemistry Laboratory, Butantan Institute, São Paulo, Brazil.
authors.forename: Gabriela D
authors.initials: GD
authors.lastname: Tanaka
authors.affiliation: Physics Institute, University of São Paulo, São Paulo, Brazil.
authors.forename: Francisco Mariano
authors.initials: FM
authors.lastname: Neto
authors.affiliation: Physics Institute, University of São Paulo, São Paulo, Brazil.
authors.forename: Paulo R A F
authors.initials: PR
authors.lastname: Garcia
authors.affiliation: Immunochemistry Laboratory, Butantan Institute, São Paulo, Brazil.
authors.forename: Joel J M
authors.initials: JJ
authors.lastname: Gabrili
authors.affiliation: Hepatitis Division, Butantan Institute, São Paulo, Brazil.
authors.forename: Denise C A
authors.initials: DC
authors.lastname: Oliveira
authors.affiliation: Immunochemistry Laboratory, Butantan Institute, São Paulo, Brazil.
authors.forename: Denise V
authors.initials: DV
authors.lastname: Tambourgi
authors.affiliation: Cristália Pharmaceutics, São Paulo, Brazil.
authors.forename: Juliana S
authors.initials: JS
authors.lastname: Mussalem
authors.affiliation: Cristália Pharmaceutics, São Paulo, Brazil.
authors.forename: Danielle
authors.initials: D
authors.lastname: Paixão-Cavalcante
authors.affiliation: Department of Physics and Chemistry, Federal University of Espírito Santo, Brazil.
authors.forename: Marcos T
authors.initials: MT
authors.lastname: D'Azeredo Orlando
authors.affiliation: Hepatitis Division, Butantan Institute, São Paulo, Brazil.
authors.forename: Viviane F
authors.initials: VF
authors.lastname: Botosso
authors.affiliation: Physics Institute, University of São Paulo, São Paulo, Brazil.
authors.forename: Cristiano L P
authors.initials: CL
authors.lastname: Oliveira
authors.affiliation: Physics Institute, University of São Paulo, São Paulo, Brazil.
authors.forename: Márcia C A
authors.initials: MC
authors.lastname: Fantini
authors.affiliation: Immunochemistry Laboratory, Butantan Institute, São Paulo, Brazil. Electronic address: gbrazil@usp.br.
authors.forename: Osvaldo A
authors.initials: OA
authors.lastname: Sant'Anna
mesh_terms: D000818:Animals; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines / Q000008:administration & dosage*; D007114:Immunization / Q000379:methods*; D051379:Mice; D012822:Silicon Dioxide; D014611:Vaccination
chemical_list: D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; C509969:SBA-15; D012822:Silicon Dioxide
keywords: Adjuvant/vehicle; Nanostructured SBA-15 silica; Oral immunization; Vaccine