title: Scale-up of naringinase production process based on the constant oxygen transfer rate for a novel strain of Bacillus methylotrophicus.
abstract: Naringinase bioprocess based on Bacillus methylotrophicus was successfully scaled up based on constant oxygen transfer rate (OTR) as the scale-up criterion from 5-L bioreactor to 20-L bioreactor. OTR was measured in 5 and 20-L bioreactor under various operating conditions using dynamic method. The operating conditions, where complete dispersion was observed were identified. The highest OTR of 0.035 and 0.04 mMol/L/s was observed in 5 and 20-L bioreactor, respectively. Critical dissolved oxygen concentration of novel isolated strain B. methylotrophicus was found to be 20% of oxygen saturation in optimized medium. The B. methylotrophicus cells grown on sucrose had maximum oxygen uptake rate of 0.14 mMol/L/s in optimized growth medium. The cells produced the maximum naringinase activity of 751 and 778 U/L at 34 hr in 5 and 20-L bioreactors, respectively. The maximum specific growth rate of about 0.178/hr was observed at both the scales of operations. The maximum naringinase yield of 160 and 164 U/g biomass was observed in 5 and 20-L bioreactors, respectively. The growth and production profiles at both scales were similar indicating successful scale-up strategy for B. methylotrophicus culture.
authors.affiliation: a Department of Chemical Engineering , National Institute of Technology Karnataka , Surathkal , Mangalore.
authors.forename: Keyur
authors.initials: K
authors.lastname: Raval
authors.affiliation: a Department of Chemical Engineering , National Institute of Technology Karnataka , Surathkal , Mangalore.
authors.forename: Kartik
authors.initials: K
authors.lastname: Gehlot
authors.affiliation: a Department of Chemical Engineering , National Institute of Technology Karnataka , Surathkal , Mangalore.
authors.forename: Prasanna
authors.initials: P
authors.lastname: B D
mesh_terms: D001407:Bacillus / Q000378:metabolism*; D018533:Biomass; D019149:Bioreactors; D009097:Multienzyme Complexes / Q000096:biosynthesis*; D010100:Oxygen / Q000378:metabolism*; D001617:beta-Glucosidase / Q000096:biosynthesis*
chemical_list: D009097:Multienzyme Complexes; C039827:naringinase; D001617:beta-Glucosidase; D010100:Oxygen
keywords: Naringinase; OTR similarity; scale-up; stirred-tank bioreactor