title: Glutathione, glutathione-related enzymes, and oxidative stress in individuals with subacute occupational exposure to lead.
abstract: The aim of the study was to investigate the influence of subacute exposure to lead on the glutathione-related antioxidant defense and oxidative stress parameters in 36 males occupationally exposed to lead for 40±3.2days. Blood lead level in the examined population increased significantly by 359% due to lead exposure. Simultaneously, erythrocyte glutathione level decreased by 16%, whereas the activity of glutathione-6-phosphate dehydrogenase in erythrocytes and leukocytes decreased by 28% and 10%, respectively. Similarly, the activity of glutathione-S-transferase in erythrocytes decreased by 45%. However, the activity of glutathione reductase in erythrocytes and leukocytes increased by 26% and 6%, respectively, whereas the total oxidant status value in leukocytes increased by 37%. Subacute exposure to lead results in glutathione pool depletion and accumulation of lipid peroxidation products; however, it does not cause DNA damage. Besides, subacute exposure to lead modifies the activity of glutathione-related enzymes.
authors.affiliation: Department of Biochemistry, School of Medicine with the Division of Dentistry, Medical University of Silesia, ul. Jordana 19, 41-808 Zabrze, Poland. Electronic address: michal.dobrakowski@poczta.fm.
authors.forename: Michał
authors.initials: M
authors.lastname: Dobrakowski
authors.affiliation: Institute of Occupational Medicine and Environmental Health in Sosnowiec, ul. Kościelna 13, 41-200 Sosnowiec, Poland.
authors.forename: Natalia
authors.initials: N
authors.lastname: Pawlas
authors.affiliation: Department of Biochemistry, School of Medicine with the Division of Dentistry, Medical University of Silesia, ul. Jordana 19, 41-808 Zabrze, Poland.
authors.forename: Edyta
authors.initials: E
authors.lastname: Hudziec
authors.affiliation: Institute of Occupational Medicine and Environmental Health in Sosnowiec, ul. Kościelna 13, 41-200 Sosnowiec, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Kozłowska
authors.affiliation: Institute of Occupational Medicine and Environmental Health in Sosnowiec, ul. Kościelna 13, 41-200 Sosnowiec, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Mikołajczyk
authors.affiliation: Department of Biochemistry, School of Medicine with the Division of Dentistry, Medical University of Silesia, ul. Jordana 19, 41-808 Zabrze, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Birkner
authors.affiliation: Department of Biochemistry, School of Medicine with the Division of Dentistry, Medical University of Silesia, ul. Jordana 19, 41-808 Zabrze, Poland.
authors.forename: Sławomir
authors.initials: S
authors.lastname: Kasperczyk
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism*; D005980:Glutathione Reductase / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism*; D006801:Humans; D007854:Lead / Q000097:blood / Q000633:toxicity*; D007962:Leukocytes / Q000187:drug effects / Q000201:enzymology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure / Q000009:adverse effects* / Q000032:analysis; D018384:Oxidative Stress / Q000187:drug effects*; D011044:Poland; D013997:Time Factors; D055815:Young Adult
chemical_list: D000975:Antioxidants; D007854:Lead; D005954:Glucosephosphate Dehydrogenase; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D005978:Glutathione
keywords: DNA damage; Glutathione; Lipid peroxidation; Oxidative stress; Subacute exposure to lead