title: Arsenic and 17-β-estradiol bind to each other and neutralize each other's signaling effects.
abstract: We report that arsenic trioxide (ATO) and 17-beta-estradiol (E2) abolish each other's independent cell signaling effects in respect of cell survival and proliferation/migration of breast cancer (MCF-7) cells. The possibility that this is due to binding of ATO to E2 was confirmed through difference absorption spectroscopy, chromatography-coupled voltammometry and 1-D (1)H and (13)C NMR spectroscopy. Binding leads to attenuation of E2's hydroxyl (1)H peaks at its C17 and C3 carbon positions. The results suggest that ATO and E2 can titrate each other's levels, potentially explaining why sustained arsenic exposure tends to be associated with delays in age of menarche, advanced age of menopause, poorer sperm quality, higher overall morbidity in men, and lower incidences of breast cancer in women in some arsenic-contaminated areas.
authors.affiliation: Center for Protein Science, Design and Engineering (CPSDE), Department of Biological Sciences, Indian Institute of Science Education and Research (IISER) Mohali, Knowledge City, Sector-81, SAS Nagar, Punjab 140306, India.
authors.forename: Sukhdeep
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Department of Biotechnology, Maharishi Markandeshwar University, Mullana, Ambala, Haryana 133207, India.
authors.forename: Tapan K
authors.initials: TK
authors.lastname: Mukherjee
authors.affiliation: Center for Protein Science, Design and Engineering (CPSDE), Department of Biological Sciences, Indian Institute of Science Education and Research (IISER) Mohali, Knowledge City, Sector-81, SAS Nagar, Punjab 140306, India. Electronic address: guptasarma@iisermohali.ac.in.
authors.forename: Purnananda
authors.initials: P
authors.lastname: Guptasarma
mesh_terms: D001151:Arsenic / Q000378:metabolism*; D001665:Binding Sites; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D004958:Estradiol / Q000378:metabolism*; D006801:Humans; D061986:MCF-7 Cells; D066244:Proton Magnetic Resonance Spectroscopy; D015398:Signal Transduction; D013057:Spectrum Analysis / Q000379:methods; D014945:Wound Healing
chemical_list: D004958:Estradiol; D001151:Arsenic
keywords: Arsenic; Endocrinology; Estrogen; Health; Molecular recognition; Reproduction