title: Generation of human control iPS cell line CHOPWT10 from healthy adult peripheral blood mononuclear cells.
abstract: The CHOPWT10 iPS cell line was generated to be used as a control for applications such as in differentiation analyses to the three germ layers and derivative tissues. Peripheral blood mononuclear cells (PBMCs) obtained from a healthy adult male were reprogrammed using the non-integrating Sendai virus expressing Oct3/4, Sox2, c-Myc, and Klf4.
authors.affiliation: Center for Cellular and Molecular Therapeutics, The Children's Hospital of Philadelphia, United States; Department of Pathology and Laboratory Medicine, The Children's Hospital of Philadelphia, University of Pennsylvania, United States.
authors.forename: Jean Ann
authors.initials: JA
authors.lastname: Maguire
authors.affiliation: Center for Cellular and Molecular Therapeutics, The Children's Hospital of Philadelphia, United States; Department of Pathology and Laboratory Medicine, The Children's Hospital of Philadelphia, University of Pennsylvania, United States.
authors.forename: Alyssa L
authors.initials: AL
authors.lastname: Gagne
authors.affiliation: Center for Cellular and Molecular Therapeutics, The Children's Hospital of Philadelphia, United States; Department of Pathology and Laboratory Medicine, The Children's Hospital of Philadelphia, University of Pennsylvania, United States.
authors.forename: Chintan D
authors.initials: CD
authors.lastname: Jobaliya
authors.affiliation: Coriell Institute for Medical Research, United States.
authors.forename: Shilpa
authors.initials: S
authors.lastname: Gandre-Babbe
authors.affiliation: Center for Cellular and Molecular Therapeutics, The Children's Hospital of Philadelphia, United States; Department of Pathology and Laboratory Medicine, The Children's Hospital of Philadelphia, University of Pennsylvania, United States.
authors.forename: Paul
authors.initials: P
authors.lastname: Gadue
authors.affiliation: Center for Cellular and Molecular Therapeutics, The Children's Hospital of Philadelphia, United States; Department of Pathology and Laboratory Medicine, The Children's Hospital of Philadelphia, University of Pennsylvania, United States.
authors.forename: Deborah L
authors.initials: DL
authors.lastname: French
mesh_terms: D000328:Adult; D002454:Cell Differentiation; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D058732:Embryoid Bodies / Q000166:cytology; D005434:Flow Cytometry; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D059785:Karyotype; D000090062:Kruppel-Like Factor 4; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D008297:Male; D008856:Microscopy, Fluorescence; D060888:Real-Time Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4; D014157:Transcription Factors