title: Bioassay-Guided Isolation of Prenylated Xanthone Derivatives from the Leaves of Garcinia oligantha.
abstract: Four new dihydroxanthone derivatives (1-4), four new tetrahydroxanthone derivatives (5-8), two new xanthone derivatives (9 and 10), and two known caged tetrahydroxanthones were isolated from extracts of the leaves of Garcinia oligantha by bioassay-guided fractionation. These structures of the new compounds were elucidated by NMR and MS spectroscopic data analysis, and the absolute configurations of compounds 1 and 5-7 were determined by electronic circular dichroism and/or single-crystal X-ray diffraction analysis. Compounds 6-9 were shown to be unusual xanthone derivatives with an isopropyl group, which was confirmed by the X-ray crystallographic structure of compound 8. The inhibitory activities of these isolates against four human tumor cell lines (A549, HepG2, HT-29, and PC-3) were assayed, and compounds 1, 2, 5, 11, and 12 showed inhibitory effects on tumor cell growth, with IC50 values ranging from 2.1 to 8.6 μM.
authors.affiliation: School of Pharmacy, Shanghai University of Traditional Chinese Medicine , Shanghai 201203, People's Republic of China.|Engineering Research Center of Shanghai Colleges for TCM New Drug Discovery , Shanghai 201203, People's Republic of China.
authors.forename: Yue-Xun
authors.initials: YX
authors.lastname: Tang
authors.affiliation: School of Pharmacy, Shanghai University of Traditional Chinese Medicine , Shanghai 201203, People's Republic of China.|Engineering Research Center of Shanghai Colleges for TCM New Drug Discovery , Shanghai 201203, People's Republic of China.
authors.forename: Wen-Wei
authors.initials: WW
authors.lastname: Fu
authors.affiliation: School of Pharmacy, Shanghai University of Traditional Chinese Medicine , Shanghai 201203, People's Republic of China.|Engineering Research Center of Shanghai Colleges for TCM New Drug Discovery , Shanghai 201203, People's Republic of China.
authors.forename: Rong
authors.initials: R
authors.lastname: Wu
authors.affiliation: School of Pharmacy, Shanghai University of Traditional Chinese Medicine , Shanghai 201203, People's Republic of China.|Engineering Research Center of Shanghai Colleges for TCM New Drug Discovery , Shanghai 201203, People's Republic of China.
authors.forename: Hong-Sheng
authors.initials: HS
authors.lastname: Tan
authors.affiliation: School of Pharmacy, Shanghai University of Traditional Chinese Medicine , Shanghai 201203, People's Republic of China.
authors.forename: Zhen-Wu
authors.initials: ZW
authors.lastname: Shen
authors.affiliation: School of Pharmacy, Shanghai University of Traditional Chinese Medicine , Shanghai 201203, People's Republic of China.|Engineering Research Center of Shanghai Colleges for TCM New Drug Discovery , Shanghai 201203, People's Republic of China.
authors.forename: Hong-Xi
authors.initials: HX
authors.lastname: Xu
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D018360:Crystallography, X-Ray; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D029760:Garcinia / Q000737:chemistry*; D019073:HT29 Cells; D056945:Hep G2 Cells; D006801:Humans; D008968:Molecular Conformation; D015394:Molecular Structure; D010696:Phloroglucinol / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D054876:Prenylation; D044004:Xanthones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D044004:Xanthones; D010696:Phloroglucinol