title: Native Piezo2 Interactomics Identifies Pericentrin as a Novel Regulator of Piezo2 in Somatosensory Neurons.
abstract: The ability of somatosensory neurons to perceive mechanical stimuli relies on specialized mechanotransducing proteins and their molecular environment. Only recently has the identity of a major transducer of mechanical forces in vertebrates been revealed by the discovery of Piezo2. Further work has established its pivotal role for innocuous touch in mice. Therefore, Piezo2 offers a unique platform for the molecular investigation of somatosensory mechanosensation. We performed a mass spectrometry-based interactomics screen on native Piezo2 in somatosensory neurons of mouse dorsal root ganglia (DRG). Stringent and quantitative data analysis yielded the identity of 36 novel binding partners of Piezo2. The biological significance of this data set is reflected by functional experiments demonstrating a role for Pericentrin in modulating Piezo2 activity and membrane expression in somatosensory neurons. Collectively, our findings provide a framework for understanding Piezo2 physiology and serve as a rich resource for the molecular dissection of mouse somatosensation.
authors.affiliation: Max-Planck Institute of Experimental Medicine , Somatosensory Signaling and Systems Biology Group, D-37075 Goettingen, Germany.
authors.forename: Pratibha
authors.initials: P
authors.lastname: Narayanan
authors.affiliation: Max-Planck Institute of Experimental Medicine , Somatosensory Signaling and Systems Biology Group, D-37075 Goettingen, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Sondermann
authors.affiliation: Max-Planck Institute of Experimental Medicine , Somatosensory Signaling and Systems Biology Group, D-37075 Goettingen, Germany.
authors.forename: Tom
authors.initials: T
authors.lastname: Rouwette
authors.affiliation: Max Planck Institute of Biophysical Chemistry , Bioanalytical Mass Spectrometry Group, D-37077 Goettingen, Germany.
authors.forename: Samir
authors.initials: S
authors.lastname: Karaca
authors.affiliation: Max Planck Institute of Biophysical Chemistry , Bioanalytical Mass Spectrometry Group, D-37077 Goettingen, Germany.|Bioanaytics Group, Institute for Clinical Chemistry, University Medical Center Göttingen , D-37075 Göttingen, Germany.
authors.forename: Henning
authors.initials: H
authors.lastname: Urlaub
authors.affiliation: Max-Planck Institute of Experimental Medicine , Light Microscopy Facility, D-37075 Goettingen, Germany.
authors.forename: Mišo
authors.initials: M
authors.lastname: Mitkovski
authors.affiliation: Max-Planck Institute of Experimental Medicine , Somatosensory Signaling and Systems Biology Group, D-37075 Goettingen, Germany.
authors.forename: David
authors.initials: D
authors.lastname: Gomez-Varela
authors.affiliation: Max-Planck Institute of Experimental Medicine , Somatosensory Signaling and Systems Biology Group, D-37075 Goettingen, Germany.
authors.forename: Manuela
authors.initials: M
authors.lastname: Schmidt
mesh_terms: D000818:Animals; D000941:Antigens / Q000378:metabolism* / Q000502:physiology; D005727:Ganglia, Spinal / Q000166:cytology; D007473:Ion Channels / Q000378:metabolism*; D040542:Mechanotransduction, Cellular; D051379:Mice; D011485:Protein Binding; D060066:Protein Interaction Maps; D013003:Somatosensory Cortex / Q000166:cytology* / Q000378:metabolism
chemical_list: D000941:Antigens; D007473:Ion Channels; C552921:Piezo2 protein, mouse; C086178:pericentrin
keywords: Piezo2; interactomics; quantitative mass spectrometry; rapidly adapting mechanically activated currents; vertebrate mechanotransduction