title: Simultaneous Biosynthesis of Polyhydroxyalkanoates and Extracellular Polymeric Substance (EPS) from Crude Glycerol from Biodiesel Production by Different Bacterial Strains.
abstract: Simultaneous synthesis of polyhydroxyalkanoates (PHAs) and polyglutamic acid (PGA) was investigated in cultures of Cupriavidus necator IPT 026, C. necator IPT 027, C. necator IPT 029, and Bacillus megaterium INCQS 425 strains in a medium containing 2.0 % sucrose or crude glycerol from biodiesel (CGB), in an orbital shaker (35 °C, 180 rpm, 72 h). All the strains tested simultaneously produced PHA and PGA in a medium containing CGB. The C. necator IPT026 culture provided higher molecular mass PHA and PGA (1128.55 and 835.56 kDa, respectively). B. megaterium INCQS 425 promoted PGA production (1.90 g L-1) with higher crystalline melting temperature (84.04 °C) and higher initial decomposition temperature (247.10 °C). Furthermore, the latter culture promoted the production of medium- and long-chain PHA (0.78 g L-1) with high crystalline melting temperatures (∼170 °C) and high initial decomposition temperature (307.53 °C) and low degree of crystallinity (20.2 %). These characteristics render these PHAs more appropriate and suitable for processes that require high temperatures, such as extrusion, increasing the possibility of industrial applications, especially in the packaging sector.
authors.affiliation: Department of Chemical Engineering, Polytechnic School, Federal University of Bahia, Aristides Novis Street, n° 2, second floor, Federação, Salvador, BA, 40210-630, Brazil. denilsonengal@gmail.com.
authors.forename: Denilson
authors.initials: D
authors.lastname: de Jesus Assis
authors.affiliation: Department of Chemical Engineering, Polytechnic School, Federal University of Bahia, Aristides Novis Street, n° 2, second floor, Federação, Salvador, BA, 40210-630, Brazil.
authors.forename: Gleice Valéria Pacheco
authors.initials: GV
authors.lastname: Gomes
authors.affiliation: Department of Bromatological Analysis, College of Pharmacy, Federal University of Bahia, Barão of Geremoabo Street, s/n, Ondina, Salvador, BA, 40171-970, Brazil.
authors.forename: Diego Roberto
authors.initials: DR
authors.lastname: da Cunha Pascoal
authors.affiliation: Department of Bromatological Analysis, College of Pharmacy, Federal University of Bahia, Barão of Geremoabo Street, s/n, Ondina, Salvador, BA, 40171-970, Brazil.
authors.forename: Lorena Silva
authors.initials: LS
authors.lastname: Pinho
authors.affiliation: Department of Bromatological Analysis, College of Pharmacy, Federal University of Bahia, Barão of Geremoabo Street, s/n, Ondina, Salvador, BA, 40171-970, Brazil.
authors.forename: Lilian Brandão Oliveira
authors.initials: LB
authors.lastname: Chaves
authors.affiliation: Department of Bromatological Analysis, College of Pharmacy, Federal University of Bahia, Barão of Geremoabo Street, s/n, Ondina, Salvador, BA, 40171-970, Brazil.
authors.forename: Janice Izabel
authors.initials: JI
authors.lastname: Druzian
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D056804:Biofuels / Q000382:microbiology*; D018533:Biomass; D001704:Biopolymers / Q000096:biosynthesis*; D002152:Calorimetry, Differential Scanning; D002851:Chromatography, High Pressure Liquid; D005110:Extracellular Space / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D005990:Glycerol / Q000378:metabolism*; D008970:Molecular Weight; D011099:Polyglutamic Acid / Q000096:biosynthesis; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis*; D012015:Reference Standards; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013818:Thermogravimetry
chemical_list: D056804:Biofuels; D001704:Biopolymers; D054813:Polyhydroxyalkanoates; D011099:Polyglutamic Acid; D005990:Glycerol
keywords: Crude glycerol; Polyglutamic acid; Polyhydroxyalkanoates; Properties; Simultaneous synthesis