title: Pathogenicity and genetic characterization of a duck Tembusu virus associated with egg-dropping in Muscovy ducks.
abstract: Duck Tembusu virus (DTMUV) has spread to the major duck-farming region in China, causing acute egg-production drop in Chinese duck population. In this study, we characterized a DTMUV strain (named GD2014) isolated from an egg-production drop duck farm in Guangdong province, South China. The virus was pathogenic to Muscovy duck embryos and caused severe egg production drop for laying Muscovy ducks. The genome sequence of GD2014 shared 97-99% homologies with other waterfowl-origin Tembusu viruses, and shared 89% identities with MM1775 strain isolated from mosquito. Phylogenetic analysis of entire open reading frame (ORF), E gene and NS5 gene indicated that GD2014 belonged to Ntaya group. These results have implications for understanding the orgin, emergence and pathogenicity of DTMUV as well as for the development of vaccines and diagnostics based on epidemiological data.
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Han-Qin
authors.initials: HQ
authors.lastname: Shen
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Wen-Cheng
authors.initials: WC
authors.lastname: Lin
authors.affiliation: Guangdong Wen's Foodstuff Group Co. Ltd, Guangdong Enterprise Key Laboratory for Animal Health and Environmental Control, Yunfu 527439, China.
authors.forename: Zhan-Xin
authors.initials: ZX
authors.lastname: Wang
authors.affiliation: Guangdong Wen's Foodstuff Group Co. Ltd, Guangdong Enterprise Key Laboratory for Animal Health and Environmental Control, Yunfu 527439, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Guangdong Wen's Foodstuff Group Co. Ltd, Guangdong Enterprise Key Laboratory for Animal Health and Environmental Control, Yunfu 527439, China.
authors.forename: Zhuan-Qiang
authors.initials: ZQ
authors.lastname: Yan
authors.affiliation: Guangdong Wen's Foodstuff Group Co. Ltd, Guangdong Enterprise Key Laboratory for Animal Health and Environmental Control, Yunfu 527439, China.
authors.forename: Qing-Feng
authors.initials: QF
authors.lastname: Zhou
authors.affiliation: Guangdong Wen's Foodstuff Group Co. Ltd, Guangdong Enterprise Key Laboratory for Animal Health and Environmental Control, Yunfu 527439, China.
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Qin
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Qing-Mei
authors.initials: QM
authors.lastname: Xie
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Ying-Zuo
authors.initials: YZ
authors.lastname: Bi
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou 510642, China. Electronic address: fengch@scau.edu.cn.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
mesh_terms: D000818:Animals; D002460:Cell Line; D004372:Ducks / Q000821:virology*; D005260:Female; D005416:Flavivirus / Q000235:genetics* / Q000472:pathogenicity*; D018177:Flavivirus Infections / Q000662:veterinary*; D016679:Genome, Viral; D016366:Open Reading Frames; D010802:Phylogeny; D011201:Poultry Diseases / Q000821:virology*; D012098:Reproduction; D017422:Sequence Analysis, DNA; D014774:Virulence
keywords: Egg production drop; Pathogenicity; Phylogenetic analysis; Tembusu virus