title: Characterizing and Mapping Resistance in Synthetic-Derived Wheat to Rhizoctonia Root Rot in a Green Bridge Environment.
abstract: Root rot caused by Rhizoctonia spp. is an economically important soilborne disease of spring-planted wheat in growing regions of the Pacific Northwest (PNW). The main method of controlling the disease currently is through tillage, which deters farmers from adopting the benefits of minimal tillage. Genetic resistance to this disease would provide an economic and environmentally sustainable resource for farmers. In this study, a collection of synthetic-derived genotypes was screened in high-inoculum and low-inoculum field environments. Six genotypes were found to have varying levels of resistance and tolerance to Rhizoctonia root rot. One of the lines, SPBC-3104 ('Vorobey'), exhibited good tolerance in the field and was crossed to susceptible PNW-adapted 'Louise' to examine the inheritance of the trait. A population of 190 BC1-derived recombinant inbred lines was assessed in two field green bridge environments and in soils artificially infested with Rhizoctonia solani AG8. Genotyping by sequencing and composite interval mapping identified three quantitative trait loci (QTL) controlling tolerance. Beneficial alleles of all three QTL were contributed by the synthetic-derived genotype SPCB-3104.
authors.affiliation: All authors: Department of Plant Pathology, Washington State University, Pullman 99164-6430; and third, fourth, and fifth authors: United States Department of Agriculture-Agricultural Research Service, Wheat Health, Genetics and Quality Research Unit, Pullman, WA 99164-6430.
authors.forename: A K
authors.initials: AK
authors.lastname: Mahoney
authors.affiliation: All authors: Department of Plant Pathology, Washington State University, Pullman 99164-6430; and third, fourth, and fifth authors: United States Department of Agriculture-Agricultural Research Service, Wheat Health, Genetics and Quality Research Unit, Pullman, WA 99164-6430.
authors.forename: E M
authors.initials: EM
authors.lastname: Babiker
authors.affiliation: All authors: Department of Plant Pathology, Washington State University, Pullman 99164-6430; and third, fourth, and fifth authors: United States Department of Agriculture-Agricultural Research Service, Wheat Health, Genetics and Quality Research Unit, Pullman, WA 99164-6430.
authors.forename: T C
authors.initials: TC
authors.lastname: Paulitz
authors.affiliation: All authors: Department of Plant Pathology, Washington State University, Pullman 99164-6430; and third, fourth, and fifth authors: United States Department of Agriculture-Agricultural Research Service, Wheat Health, Genetics and Quality Research Unit, Pullman, WA 99164-6430.
authors.forename: D
authors.initials: D
authors.lastname: See
authors.affiliation: All authors: Department of Plant Pathology, Washington State University, Pullman 99164-6430; and third, fourth, and fifth authors: United States Department of Agriculture-Agricultural Research Service, Wheat Health, Genetics and Quality Research Unit, Pullman, WA 99164-6430.
authors.forename: P A
authors.initials: PA
authors.lastname: Okubara
authors.affiliation: All authors: Department of Plant Pathology, Washington State University, Pullman 99164-6430; and third, fourth, and fifth authors: United States Department of Agriculture-Agricultural Research Service, Wheat Health, Genetics and Quality Research Unit, Pullman, WA 99164-6430.
authors.forename: S H
authors.initials: SH
authors.lastname: Hulbert
mesh_terms: D000483:Alleles; D002874:Chromosome Mapping / Q000379:methods*; D032461:Chromosomes, Plant / Q000235:genetics*; D060467:Disease Resistance / Q000235:genetics*; D004777:Environment; D005838:Genotype; D060005:Genotyping Techniques; D015205:Northwestern United States; D010641:Phenotype; D010935:Plant Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D018517:Plant Roots / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D012232:Rhizoctonia / Q000502:physiology*; D017422:Sequence Analysis, DNA; D014908:Triticum / Q000235:genetics* / Q000276:immunology / Q000382:microbiology