title: Three new trixane glycosides obtained from the leaves of Jungia sellowii Less. using centrifugal partition chromatography.
abstract: Jungia sellowii (Asteraceae) is a shrub that grows in Southern Brazil and polar extract of its leaves presents anti-inflammatory properties. Cyperane, guaiane, nortrixane, and trixane sesquiterpene types were reported as the main metabolites in Jungia species. This work aims to describe the isolation and identification of sesquiterpenes in the leaves of J. sellowii using liquid-liquid partition and centrifugal partition chromatography. Thus, the crude extract of fresh leaves of J. sellowii was partitioned with hexane, dichloromethane, ethyl acetate and butanol, respectively. The butanol fraction was then subjected to a selected ternary system optimized for the CPC (centrifugal partition chromatography): ethyl acetate-ethanol-water (9:2:10, v/v/v). The separation was carried out isocratically at a flow rate of 25 mL/min at 1200 rpm, affording seven fractions A to G. TLC of fractions B, C and F displayed a single spot corresponding to three new glycosylated sesquiterpenoids. Their structures were established by using spectroscopic data in comparison to those reported in the literature. Furthermore, the isolates were evaluated for their leishmanicidal and cytotoxic effects. No cytotoxic effect was observed against the three cancer cell lines (HL60, JURKAT and REH), but compound 1 showed a weak antiprotozoal activity. Liquid-liquid partition and CPC turned to be a versatile technique of glycoside purification which is environmentally friendly and requires a limited amount of organic solvents.
authors.affiliation: Programa de Pós-Graduação em Farmácia, Universidade Federal de Santa Catarina, UFSC, Florianópolis, SC, Brazil.
authors.forename: Luíse
authors.initials: L
authors.lastname: Azevedo
authors.affiliation: Programa de Pós-Graduação em Farmácia, Universidade Federal de Santa Catarina, UFSC, Florianópolis, SC, Brazil.
authors.forename: Larissa
authors.initials: L
authors.lastname: Faqueti
authors.affiliation: Laboratoire de Pharmacognosie UMR/CNRS 8638 COMETE, Université Paris Descartes, Sorbonne Paris Cité, Faculté des Sciences Pharmaceutiques et Biologiques, 4 Avenue de l'observatoire 75006 Paris, France.
authors.forename: Marina
authors.initials: M
authors.lastname: Kritsanida
authors.affiliation: Laboratory of Molecular Parasitology, Department of Microbiology, Hellenic Pasteur Institute, 127 Vas. Sofias Ave, 11521 Athens, Greece.
authors.forename: Antonia
authors.initials: A
authors.lastname: Efstathiou
authors.affiliation: Laboratory of Molecular Parasitology, Department of Microbiology, Hellenic Pasteur Institute, 127 Vas. Sofias Ave, 11521 Athens, Greece.
authors.forename: Despina
authors.initials: D
authors.lastname: Smirlis
authors.affiliation: Integrated Center for Childhood Onco-Hematological Investigation, State University of Campinas, P.O. Box 6141, 13083-970 Campinas, SP, Brazil.
authors.forename: Gilberto C
authors.initials: GC
authors.lastname: Franchi
authors.affiliation: Laboratoire de Pharmacognosie UMR/CNRS 8638 COMETE, Université Paris Descartes, Sorbonne Paris Cité, Faculté des Sciences Pharmaceutiques et Biologiques, 4 Avenue de l'observatoire 75006 Paris, France.
authors.forename: Grégory
authors.initials: G
authors.lastname: Genta-Jouve
authors.affiliation: Laboratoire de Pharmacognosie UMR/CNRS 8638 COMETE, Université Paris Descartes, Sorbonne Paris Cité, Faculté des Sciences Pharmaceutiques et Biologiques, 4 Avenue de l'observatoire 75006 Paris, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Michel
authors.affiliation: Programa de Pós-Graduação em Farmácia, Universidade Federal de Santa Catarina, UFSC, Florianópolis, SC, Brazil.
authors.forename: Louis P
authors.initials: LP
authors.lastname: Sandjo
authors.affiliation: Laboratoire de Pharmacognosie UMR/CNRS 8638 COMETE, Université Paris Descartes, Sorbonne Paris Cité, Faculté des Sciences Pharmaceutiques et Biologiques, 4 Avenue de l'observatoire 75006 Paris, France.
authors.forename: Raphaël
authors.initials: R
authors.lastname: Grougnet
authors.affiliation: Programa de Pós-Graduação em Farmácia, Universidade Federal de Santa Catarina, UFSC, Florianópolis, SC, Brazil.
authors.forename: Maique Weber
authors.initials: MW
authors.lastname: Biavatti
keywords: CPC; Jungia; guaiane; trixanolide