title: Study of peripheral stem cells mobilization as a treatment line of pediatric dilated cardiomyopathy.
abstract: BACKGROUND
  Mobilizing hematopoietic stem cells may be a promising intervention for the treatment of idiopathic dilated cardiomyopathy (IDCM) in infant and children. So the aim of the work is to evaluate the efficacy of granulocyte-colony stimulating factor (G-CSF) as a therapeutic modality in pediatric IDCM.
  
  
  METHODS
  A randomized clinical trial was conducted on 40 pediatric patients with IDCM. They were subjected to history taking, clinical examination, serum lactate dehydrogenase (LDH), total creatinine phosphokinase (CPK), creatinine phosphokinase isoenzyme B (CK-MB) isoenzyme, and peripheral blood CD34(+) cell assessment before and at day 7 after subcutaneous G-CSF injection for 5 consecutive days. Echocardiography was done before and 1, 3 and 6 months after therapy.
  
  
  RESULTS
  Clinical improvement in the form of regression of patients Modified Ross heart failure (MRHC) classification classes. Increased percentage of CD34(+) mobilized cells from the bone marrow, and significant increase in blood counts especially white blood cells 7 days after G-CSF injection. Significant improvement was found in echocardiographic data evaluating systolic function of the heart [Ejection fraction, Fractional shortening and systolic velocity at mitral annulus (Sm)].
  
  
  CONCLUSIONS
  Administration of G-CSF may be beneficial in improving systolic functions of the heart in pediatric IDCM and more studies with a large number of patients are needed.
authors.affiliation: 1 Department of Pediatrics, 2 Department of Clinical Pathology, 3 Department of Cardiology, Faculty of Medicine, Tanta University, Tanta, Egypt.
authors.forename: Mohamed
authors.initials: M
authors.lastname: El-Shanshory
authors.affiliation: 1 Department of Pediatrics, 2 Department of Clinical Pathology, 3 Department of Cardiology, Faculty of Medicine, Tanta University, Tanta, Egypt.
authors.forename: Waled
authors.initials: W
authors.lastname: El-Shehaby
authors.affiliation: 1 Department of Pediatrics, 2 Department of Clinical Pathology, 3 Department of Cardiology, Faculty of Medicine, Tanta University, Tanta, Egypt.
authors.forename: Nahed
authors.initials: N
authors.lastname: Hables
authors.affiliation: 1 Department of Pediatrics, 2 Department of Clinical Pathology, 3 Department of Cardiology, Faculty of Medicine, Tanta University, Tanta, Egypt.
authors.forename: Said
authors.initials: S
authors.lastname: Hamad
authors.affiliation: 1 Department of Pediatrics, 2 Department of Clinical Pathology, 3 Department of Cardiology, Faculty of Medicine, Tanta University, Tanta, Egypt.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Attia
authors.affiliation: 1 Department of Pediatrics, 2 Department of Clinical Pathology, 3 Department of Cardiology, Faculty of Medicine, Tanta University, Tanta, Egypt.
authors.forename: Ayman
authors.initials: A
authors.lastname: El-Said
keywords: Granulocyte-colony stimulating factor (G-CSF); idiopathic dilated cardiomyopathy (IDCM); pediatrics