title: Validation of QuEChERS method for the determination of some pesticide residues in two apple varieties.
abstract: This study was undertaken to validate the "quick, easy, cheap, effective, rugged and safe" (QuEChERS) method using Golden Delicious and Starking Delicious apple matrices spiked at 0.1 maximum residue limit (MRL), 1.0 MRL and 10 MRL levels of the four pesticides (chlorpyrifos, dimethoate, indoxacarb and imidacloprid). For the extraction and cleanup, original QuEChERS method was followed, then the samples were subjected to liquid chromatography-triple quadrupole mass spectrometry (LC-MS/MS) for chromatographic analyses. According to t test, matrix effect was not significant for chlorpyrifos in both sample matrices, but it was significant for dimethoate, indoxacarb and imidacloprid in both sample matrices. Thus, matrix-matched calibration (MC) was used to compensate matrix effect and quantifications were carried out by using MC. The overall recovery of the method was 90.15% with a relative standard deviation of 13.27% (n = 330). Estimated method detection limit of analytes blew the MRLs. Some other parameters of the method validation, such as recovery, precision, accuracy and linearity were found to be within the required ranges.
authors.affiliation: a Department of Plant Protection , Faculty of Agriculture, Çanakkale Onsekiz Mart University , Çanakkale , Turkey.
authors.forename: Osman
authors.initials: O
authors.lastname: Tiryaki
mesh_terms: D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D057230:Limit of Detection; D027845:Malus / Q000737:chemistry*; D010573:Pesticide Residues / Q000032:analysis*; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D010573:Pesticide Residues
keywords: Liquid chromatograph-mass spectrometer (LC-MS/MS); QuEChERS; matrix effect; pesticides; validation