title: A high-density genetic map and growth related QTL mapping in bighead carp (Hypophthalmichthys nobilis).
abstract: Growth related traits in fish are controlled by quantitative trait loci (QTL), but no QTL for growth have been detected in bighead carp (Hypophthalmichthys nobilis) due to the lack of high-density genetic map. In this study, an ultra-high density genetic map was constructed with 3,121 SNP markers by sequencing 117 individuals in a F1 family using 2b-RAD technology. The total length of the map was 2341.27 cM, with an average marker interval of 0.75 cM. A high level of genomic synteny between our map and zebrafish was detected. Based on this genetic map, one genome-wide significant and 37 suggestive QTL for five growth-related traits were identified in 6 linkage groups (i.e. LG3, LG11, LG15, LG18, LG19, LG22). The phenotypic variance explained (PVE) by these QTL varied from 15.4% to 38.2%. Marker within the significant QTL region was surrounded by CRP1 and CRP2, which played an important role in muscle cell division. These high-density map and QTL information provided a solid base for QTL fine mapping and comparative genomics in bighead carp.
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072,China.
authors.forename: Beide
authors.initials: B
authors.lastname: Fu
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072,China.|University of Chinese Academy of Sciences, Beijing 100039, China.
authors.forename: Haiyang
authors.initials: H
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072,China.
authors.forename: Xiaomu
authors.initials: X
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072,China.
authors.forename: Jingou
authors.initials: J
authors.lastname: Tong
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D003530:Cyprinidae / Q000235:genetics*; D008040:Genetic Linkage; D055106:Genome-Wide Association Study; D010641:Phenotype; D019655:Quantitative Trait, Heritable