title: Structure-function analysis of the extracellular domain of the pneumococcal cell division site positioning protein MapZ.
abstract: Accurate placement of the bacterial division site is a prerequisite for the generation of two viable and identical daughter cells. In Streptococcus pneumoniae, the positive regulatory mechanism involving the membrane protein MapZ positions precisely the conserved cell division protein FtsZ at the cell centre. Here we characterize the structure of the extracellular domain of MapZ and show that it displays a bi-modular structure composed of two subdomains separated by a flexible serine-rich linker. We further demonstrate in vivo that the N-terminal subdomain serves as a pedestal for the C-terminal subdomain, which determines the ability of MapZ to mark the division site. The C-terminal subdomain displays a patch of conserved amino acids and we show that this patch defines a structural motif crucial for MapZ function. Altogether, this structure-function analysis of MapZ provides the first molecular characterization of a positive regulatory process of bacterial cell division.
authors.affiliation: CNRS, Molecular Microbiology and Structural Biochemistry, UMR 5086, 7 passage du Vercors, Lyon 69367, France.|Université Lyon 1, Molecular Microbiology and Structural Biochemistry, UMR 5086, 7 passage du Vercors, Lyon 69367, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Manuse
authors.affiliation: Université Grenoble Alpes, Institut de Biologie Structurale, 71 avenue des Martyrs-CS10090, Grenoble cedex 9 38044, France.|CEA, DSV, Institut de Biologie Structurale, 71 avenue des Martyrs-CS10090, Grenoble cedex 9 38044, France.|CNRS, Institut de Biologie Structurale, 71 avenue des Martyrs-CS10090, Grenoble cedex 9 38044, France.
authors.forename: Nicolas L
authors.identifier: 0000-0001-7759-4757
authors.initials: NL
authors.lastname: Jean
authors.affiliation: CNRS, Molecular Microbiology and Structural Biochemistry, UMR 5086, 7 passage du Vercors, Lyon 69367, France.|Université Lyon 1, Molecular Microbiology and Structural Biochemistry, UMR 5086, 7 passage du Vercors, Lyon 69367, France.
authors.forename: Mégane
authors.initials: M
authors.lastname: Guinot
authors.affiliation: CNRS, Molecular Microbiology and Structural Biochemistry, UMR 5086, 7 passage du Vercors, Lyon 69367, France.|Université Lyon 1, Molecular Microbiology and Structural Biochemistry, UMR 5086, 7 passage du Vercors, Lyon 69367, France.
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Lavergne
authors.affiliation: Université Grenoble Alpes, Institut de Biologie Structurale, 71 avenue des Martyrs-CS10090, Grenoble cedex 9 38044, France.|CEA, DSV, Institut de Biologie Structurale, 71 avenue des Martyrs-CS10090, Grenoble cedex 9 38044, France.|CNRS, Institut de Biologie Structurale, 71 avenue des Martyrs-CS10090, Grenoble cedex 9 38044, France.
authors.forename: Cédric
authors.initials: C
authors.lastname: Laguri
authors.affiliation: Université Grenoble Alpes, Institut de Biologie Structurale, 71 avenue des Martyrs-CS10090, Grenoble cedex 9 38044, France.|CEA, DSV, Institut de Biologie Structurale, 71 avenue des Martyrs-CS10090, Grenoble cedex 9 38044, France.|CNRS, Institut de Biologie Structurale, 71 avenue des Martyrs-CS10090, Grenoble cedex 9 38044, France.
authors.forename: Catherine M
authors.initials: CM
authors.lastname: Bougault
authors.affiliation: Department of Chemistry, Indiana University, 800 E. Kirkwood Avenue, Bloomington, Indiana 47405-7102, USA.
authors.forename: Michael S
authors.initials: MS
authors.lastname: VanNieuwenhze
authors.affiliation: CNRS, Molecular Microbiology and Structural Biochemistry, UMR 5086, 7 passage du Vercors, Lyon 69367, France.|Université Lyon 1, Molecular Microbiology and Structural Biochemistry, UMR 5086, 7 passage du Vercors, Lyon 69367, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Grangeasse
authors.affiliation: Université Grenoble Alpes, Institut de Biologie Structurale, 71 avenue des Martyrs-CS10090, Grenoble cedex 9 38044, France.|CEA, DSV, Institut de Biologie Structurale, 71 avenue des Martyrs-CS10090, Grenoble cedex 9 38044, France.|CNRS, Institut de Biologie Structurale, 71 avenue des Martyrs-CS10090, Grenoble cedex 9 38044, France.
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Simorre
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division / Q000502:physiology; D048749:Cytokinesis; D003598:Cytoskeletal Proteins / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D008958:Models, Molecular; D011487:Protein Conformation; D000072417:Protein Domains; D013296:Streptococcus pneumoniae / Q000378:metabolism*; D013329:Structure-Activity Relationship
chemical_list: D001426:Bacterial Proteins; D003598:Cytoskeletal Proteins