title: miR-422a is an independent prognostic factor and functions as a potential tumor suppressor in colorectal cancer.
abstract: OBJECTIVE
  To determine the expression of miR-422a in colorectal cancer (CRC) tissues and to further explore the prognostic value and function of miR-422a in CRC carcinogenesis.
  
  
  METHODS
  miR-422a expression was analyzed in 102 CRC tissues and paired normal mucosa adjacent to carcinoma by quantitative real-time PCR. The relationship of miR-422a expression with clinicopathological parameters was also analyzed. Kaplan-Meier analysis and Cox multivariate analysis were performed to estimate the potential role of miR-422a. Cell proliferation, migration, and invasion were used for in vitro functional analysis of miR-422a.
  
  
  RESULTS
  The levels of miR-422a were dramatically reduced in CRC tissues compared with normal mucosa (P < 0.05), and significantly correlated with local invasion (P = 0.004) and lymph node metastasis (P < 0.001). Kaplan-Meier survival and Cox regression multivariate analyses revealed that miR-422a expression (HR = 0.568, P = 0.015) and clinical TNM stage (HR = 2.942, P = 0.003) were independent prognostic factors for overall survival in CRC patients. Furthermore, in vitro experiments showed that overexpression of miR-422a inhibited the proliferation, migration, and invasion of SW480 and HT-29 cells.
  
  
  CONCLUSIONS
  Down-regulation of miR-422a may serve as an independent prognosis factor in CRC. MiR-422a functions as a tumor suppressor and regulates progression of CRC.
authors.affiliation: Gui-Xi Zheng, Ai-Lin Qu, Yong-Mei Yang, Xin Zhang, Shou-Cai Zhang, Chuan-Xin Wang, Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan 250012, Shandong Province, China.
authors.forename: Gui-Xi
authors.initials: GX
authors.lastname: Zheng
authors.affiliation: Gui-Xi Zheng, Ai-Lin Qu, Yong-Mei Yang, Xin Zhang, Shou-Cai Zhang, Chuan-Xin Wang, Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan 250012, Shandong Province, China.
authors.forename: Ai-Lin
authors.initials: AL
authors.lastname: Qu
authors.affiliation: Gui-Xi Zheng, Ai-Lin Qu, Yong-Mei Yang, Xin Zhang, Shou-Cai Zhang, Chuan-Xin Wang, Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan 250012, Shandong Province, China.
authors.forename: Yong-Mei
authors.initials: YM
authors.lastname: Yang
authors.affiliation: Gui-Xi Zheng, Ai-Lin Qu, Yong-Mei Yang, Xin Zhang, Shou-Cai Zhang, Chuan-Xin Wang, Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan 250012, Shandong Province, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Gui-Xi Zheng, Ai-Lin Qu, Yong-Mei Yang, Xin Zhang, Shou-Cai Zhang, Chuan-Xin Wang, Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan 250012, Shandong Province, China.
authors.forename: Shou-Cai
authors.initials: SC
authors.lastname: Zhang
authors.affiliation: Gui-Xi Zheng, Ai-Lin Qu, Yong-Mei Yang, Xin Zhang, Shou-Cai Zhang, Chuan-Xin Wang, Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan 250012, Shandong Province, China.
authors.forename: Chuan-Xin
authors.initials: CX
authors.lastname: Wang
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism; D005260:Female; D019073:HT29 Cells; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D015999:Multivariate Analysis; D009361:Neoplasm Invasiveness; D011379:Prognosis; D016016:Proportional Hazards Models; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C574206:MIRN422 microRNA, human; D035683:MicroRNAs
keywords: Colorectal cancer; In vitro function; MicroRNA; Prognosis; miR-422a