title: Unlocking the bacterial and fungal communities assemblages of sugarcane microbiome.
abstract: Plant microbiome and its manipulation herald a new era for plant biotechnology with the potential to benefit sustainable crop production. However, studies evaluating the diversity, structure and impact of the microbiota in economic important crops are still rare. Here we describe a comprehensive inventory of the structure and assemblage of the bacterial and fungal communities associated with sugarcane. Our analysis identified 23,811 bacterial OTUs and an unexpected 11,727 fungal OTUs inhabiting the endophytic and exophytic compartments of roots, shoots, and leaves. These communities originate primarily from native soil around plants and colonize plant organs in distinct patterns. The sample type is the primary driver of fungal community assemblage, and the organ compartment plays a major role in bacterial community assemblage. We identified core bacterial and fungal communities composed of less than 20% of the total microbial richness but accounting for over 90% of the total microbial relative abundance. The roots showed 89 core bacterial families, 19 of which accounted for 44% of the total relative abundance. Stalks are dominated by groups of yeasts that represent over 12% of total relative abundance. The core microbiome described here comprise groups whose biological role underlies important traits in plant growth and fermentative processes.
authors.affiliation: Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas (UNICAMP), 13083-875, Campinas, SP, Brazil.
authors.forename: Rafael Soares Correa
authors.initials: RS
authors.lastname: de Souza
authors.affiliation: Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas (UNICAMP), 13083-875, Campinas, SP, Brazil.
authors.forename: Vagner Katsumi
authors.initials: VK
authors.lastname: Okura
authors.affiliation: Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas (UNICAMP), 13083-875, Campinas, SP, Brazil.
authors.forename: Jaderson Silveira Leite
authors.initials: JS
authors.lastname: Armanhi
authors.affiliation: Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM) - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA Campus Montegancedo UPM, 28223 -Pozuelo de Alarcón (Madrid), Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Jorrín
authors.affiliation: Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM) - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA Campus Montegancedo UPM, 28223 -Pozuelo de Alarcón (Madrid), Spain.
authors.forename: Núria
authors.initials: N
authors.lastname: Lozano
authors.affiliation: Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas (UNICAMP), 13083-875, Campinas, SP, Brazil.
authors.forename: Márcio José
authors.initials: MJ
authors.lastname: da Silva
authors.affiliation: Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM) - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA Campus Montegancedo UPM, 28223 -Pozuelo de Alarcón (Madrid), Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: González-Guerrero
authors.affiliation: Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas (UNICAMP), 13083-875, Campinas, SP, Brazil.
authors.forename: Laura Migliorini
authors.initials: LM
authors.lastname: de Araújo
authors.affiliation: Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas (UNICAMP), 13083-875, Campinas, SP, Brazil.
authors.forename: Natália Cristina
authors.initials: NC
authors.lastname: Verza
authors.affiliation: New Energies Division, Repsol Technology Center, 28935 Mostoles-Madrid, Spain.
authors.forename: Homayoun Chaichian
authors.initials: HC
authors.lastname: Bagheri
authors.affiliation: Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM) - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA Campus Montegancedo UPM, 28223 -Pozuelo de Alarcón (Madrid), Spain.|Consejo Superior de Investigaciones Científicas, Madrid, Spain.
authors.forename: Juan
authors.initials: J
authors.lastname: Imperial
authors.affiliation: Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas (UNICAMP), 13083-875, Campinas, SP, Brazil.|Departamento de Genética e Evolução, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), 13083-970, Campinas, SP, Brazil.
authors.forename: Paulo
authors.initials: P
authors.lastname: Arruda
mesh_terms: D018407:Bacterial Physiological Phenomena / Q000235:genetics; D044822:Biodiversity; D016000:Cluster Analysis; D005658:Fungi / Q000502:physiology*; D064307:Microbiota; D018515:Plant Leaves / Q000382:microbiology; D018517:Plant Roots / Q000382:microbiology; D018520:Plant Shoots / Q000382:microbiology; D025341:Principal Component Analysis; D012336:RNA, Ribosomal, 16S / Q000145:classification / Q000235:genetics; D058441:Rhizosphere; D031786:Saccharum / Q000382:microbiology*; D012988:Soil Microbiology
chemical_list: D012336:RNA, Ribosomal, 16S