title: Identification of Infantile Diarrhea Caused by Breast Milk-Transmitted Staphylococcus aureus Infection.
abstract: Staphylococcus aureus is a well-known organism which is responsible for a variety of human infectious diseases including skin infections, pneumonia, bacteremia, and endocarditis. Few of the microorganisms can be transmitted from mother to the newborn or infant by milk breastfeeding. This study aims to identify transmission of S. aureus from healthy, lactating mothers to their infants by breastfeeding. Stool specimens of diarrheal infants and breast milk of their mother (totally three pairs) were collected and six Staphylococcus aureus isolates were cultured positively. Homology and molecular characters of isolated strains were tested using pulsed-field gel electrophoresis (PFGE), spa typing, and multilocus sequence typing. Furthermore, toxin genes detection was also performed. Each pair of isolates has the same PFGE type and spa type. Four Sequence types (STs) were found among all the isolates; they are ST15, ST188, and ST59, respectively. Among the strains, seb, sec, and tst genes were found, and all were negative for pvl gene. The homology of the S. aureus strains isolated from the infants' stool and the mothers' milk was genetically demonstrated, which indicated that breastfeeding may be important in the transmission of S. aureus infection, and the character of S. aureus needed to be further evaluated.
authors.affiliation: Department of Infectious Diseases and Shenzhen Key Lab for Endogenous infection, The Affiliated Shenzhen Nanshan Hospital, Guangdong Medical University, No 89, Taoyuan Road, Nanshan district, Shenzhen, 518052, China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Infectious Diseases and Shenzhen Key Lab for Endogenous infection, The Affiliated Shenzhen Nanshan Hospital, Guangdong Medical University, No 89, Taoyuan Road, Nanshan district, Shenzhen, 518052, China.
authors.forename: Wei-Guang
authors.initials: WG
authors.lastname: Pan
authors.affiliation: Yan-tian Community health service center, Ban'an Central Hospital of Shenzhen Affiliated to Guangdong Medical University, No 63, Yantian Road, Bao'an district, Shenzhen, 518102, China.
authors.forename: Wei-Yi
authors.initials: WY
authors.lastname: Xian
authors.affiliation: Department of Infectious Diseases and Shenzhen Key Lab for Endogenous infection, The Affiliated Shenzhen Nanshan Hospital, Guangdong Medical University, No 89, Taoyuan Road, Nanshan district, Shenzhen, 518052, China.
authors.forename: Hang
authors.initials: H
authors.lastname: Cheng
authors.affiliation: Department of Infectious Diseases and Shenzhen Key Lab for Endogenous infection, The Affiliated Shenzhen Nanshan Hospital, Guangdong Medical University, No 89, Taoyuan Road, Nanshan district, Shenzhen, 518052, China.
authors.forename: Jin-Xin
authors.initials: JX
authors.lastname: Zheng
authors.affiliation: Department of Microbiology Laboratory, Shenzhen Center for Disease Control and Prevention, No 8, Longyuan Road, Nanshan district, Shenzhen, 518055, China.
authors.forename: Qing-Hua
authors.initials: QH
authors.lastname: Hu
authors.affiliation: Department of Infectious Diseases and Shenzhen Key Lab for Endogenous infection, The Affiliated Shenzhen Nanshan Hospital, Guangdong Medical University, No 89, Taoyuan Road, Nanshan district, Shenzhen, 518052, China. yuzhijiansmu@163.com.
authors.forename: Zhi-Jian
authors.initials: ZJ
authors.lastname: Yu
authors.affiliation: Department of Infectious Diseases and Shenzhen Key Lab for Endogenous infection, The Affiliated Shenzhen Nanshan Hospital, Guangdong Medical University, No 89, Taoyuan Road, Nanshan district, Shenzhen, 518052, China. qiwendeng@hotmail.com.
authors.forename: Qi-Wen
authors.initials: QW
authors.lastname: Deng
mesh_terms: D000328:Adult; D001942:Breast Feeding / Q000009:adverse effects; D002681:China; D003968:Diarrhea, Infantile / Q000209:etiology / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D018445:Infectious Disease Transmission, Vertical; D008297:Male; D008895:Milk, Human / Q000382:microbiology*; D058885:Multilocus Sequence Typing; D013203:Staphylococcal Infections / Q000209:etiology / Q000382:microbiology* / Q000635:transmission; D013211:Staphylococcus aureus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D055815:Young Adult