title: A ratiometric two-photon fluorescent probe for fluoride ion imaging in living cells and zebrafish.
abstract: Using 6-hydroxyl-quinoline-2-benzothiazole (HQB) as a two-photon fluorophore and tert-butyldiphenylsilyl as a recognition domain for F(-), a ratiometric two-photon fluorescent fluoride probe, QF, was synthesized and fully characterized. QF displays both one- and two-photon ratiometric responses towards fluoride ions in aqueous solution. QF was enabled to detect exogenous fluoride ions in living cells by a ratiometric method. Two-photon microscopic imaging of fluoride ions in living HeLa cells and zebrafish has also been achieved. QF has been demonstrated to be an excellent fluorescent probe with high selectivity, low cytotoxicity and good photostability.
authors.affiliation: Key Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry and Material Science, South-central University of Nationalities, Wuhan 430074, China. lcychem@yahoo.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Hu
authors.forename: Lingyu
authors.initials: L
authors.lastname: Zeng
authors.forename: Yanying
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Liu
authors.forename: Xiaoxue
authors.initials: X
authors.lastname: Ye
authors.forename: Chunya
authors.initials: C
authors.lastname: Li
mesh_terms: D000818:Animals; D005456:Fluorescent Dyes; D005459:Fluorides / Q000032:analysis*; D006367:HeLa Cells; D006801:Humans; D008853:Microscopy; D017785:Photons; D015027:Zebrafish
chemical_list: D005456:Fluorescent Dyes; D005459:Fluorides