title: Isolation and Culture of Human Endometrial Epithelial Cells and Stromal Fibroblasts.
abstract: Purification and culture of endometrial epithelial cells (eEC) and stromal fibroblasts (eSF) from endometrial biopsies allows for downstream cell-specificin vitrostudies. The utility of this protocol is the ease with which cells are purified without contamination from unwanted cell types, and the ability to use patient-paired eEC and eSF in experiments. These methods have been previously published, but here the protocol has been updated for maximum efficiency.
authors.affiliation: Center for Reproductive Sciences, Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, USA.
authors.forename: Joseph C
authors.initials: JC
authors.lastname: Chen
authors.affiliation: Department of Urology, University of California, San Francisco, USA, and Gladstone Institute of Virology and Immunology, San Francisco, USA.
authors.forename: Nadia R
authors.initials: NR
authors.lastname: Roan