title: Impact of inhibitory peptides released by Saccharomyces cerevisiae BDX on the malolactic fermentation performed by Oenococcus oeni Vitilactic F.
abstract: A previous study has shown that the malolactic fermentation (MLF) was inhibited during sequential fermentations performed with the pair Saccharomyces cerevisiae BDX/Oenococcus oeni Vitilactic F in synthetic grape juices. A yeast peptidic fraction with an apparent MW of 5-10kDa was involved in the inhibition. In the present study, the MLF was also inhibited in Cabernet Sauvignon and Syrah wines. The inhibition due to the peptidic fraction was maintained despite high phenolic contents. Kinetic studies showed that the peptidic fraction was gradually released during the alcoholic fermentation (AF). Its highest anti-MLF effect was reached when isolated from late stages of the AF stationary phase. The peptidic fraction was tested in vitro on cell-free bacterial cytosolic extracts containing the malolactic enzyme in a pH range between 3.5 and 6.7. Results showed that it was able to directly inhibit the malolactic enzyme activity with an increasing inhibitory kinetic correlated to the AF time at which it was collected.
authors.affiliation: Lebanese Agricultural Research Institute (LARI), Fanar Station, P.O. Box 90-1965, Jdeidet El-Metn, Fanar, Lebanon; Faculty of Sciences, Holy Spirit University of Kaslik, P.O. Box 446, Jounieh, Lebanon; Laboratoire de Génie Chimique, UMR CNRS 5503, INPT-ENSAT, UPS, Université de Toulouse, 1 Avenue de l'Agrobiopôle, F-31326 Castanet-Tolosan, France. Electronic address: ziadrizk_ce@hotmail.com.
authors.forename: Ziad
authors.initials: Z
authors.lastname: Rizk
authors.affiliation: Faculty of Agricultural and Food Sciences, Holy Spirit University of Kaslik, P.O. Box 446, Jounieh, Lebanon. Electronic address: youssefrayess@usek.edu.lb.
authors.forename: Youssef
authors.initials: Y
authors.lastname: El Rayess
authors.affiliation: Lebanese Agricultural Research Institute (LARI), Fanar Station, P.O. Box 90-1965, Jdeidet El-Metn, Fanar, Lebanon; Faculty of Agricultural and Food Sciences, Holy Spirit University of Kaslik, P.O. Box 446, Jounieh, Lebanon.
authors.forename: Chantal
authors.initials: C
authors.lastname: Ghanem
authors.affiliation: Laboratoire de Génie Chimique, UMR CNRS 5503, INPT-ENSAT, UPS, Université de Toulouse, 1 Avenue de l'Agrobiopôle, F-31326 Castanet-Tolosan, France. Electronic address: mathieu@ensat.fr.
authors.forename: Florence
authors.initials: F
authors.lastname: Mathieu
authors.affiliation: Laboratoire de Génie Chimique, UMR CNRS 5503, INPT-ENSIACET, UPS, Université de Toulouse, 4 Allée Emile Monso, F-31432 Toulouse, France. Electronic address: patricia.taillandier@ensiacet.fr.
authors.forename: Patricia
authors.initials: P
authors.lastname: Taillandier
authors.affiliation: Faculty of Sciences, Holy Spirit University of Kaslik, P.O. Box 446, Jounieh, Lebanon; Faculty of Agricultural Engineering and Veterinary Medicine, Lebanese University, Dekwaneh, Lebanon. Electronic address: nehmenancy@hotmail.com.
authors.forename: Nancy
authors.initials: N
authors.lastname: Nehme
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D005285:Fermentation; D007700:Kinetics; D008291:Malate Dehydrogenase / Q000737:chemistry / Q000378:metabolism; D008293:Malates / Q000378:metabolism*; D056585:Oenococcus / Q000187:drug effects / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000378:metabolism; D027843:Vitis / Q000378:metabolism / Q000382:microbiology; D014920:Wine / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D008293:Malates; D010455:Peptides; C037731:malolactic enzyme; D008291:Malate Dehydrogenase
keywords: Antibacterial yeast peptidic compounds; Malolactic enzyme; Oenococcus oeni; Saccharomyces cerevisiae