title: Low-molecular-weight fractions of Alcalase hydrolyzed egg ovomucin extract exert anti-inflammatory activity in human dermal fibroblasts through the inhibition of tumor necrosis factor-mediated nuclear factor κB pathway.
abstract: Ovomucin is a mucin-like protein from egg white with a variety of biological functions. We hypothesized that ovomucin-derived peptides might exert anti-inflammatory activity. The specific objectives were to test the anti-inflammatory activities of different ovomucin hydrolysates and its various fractions in human dermal fibroblasts, and to understand the possible molecular mechanisms. Three ovomucin hydrolysates were prepared and desalted; only the desalted Alcalase hydrolysate showed anti-inflammatory activity. Desalting of ovomucin hydrolysate enriched the proportion of low-molecular-weight (MW) peptides. Indeed, ultrafiltration of this hydrolysate displayed comparable anti-inflammatory activity in dermal fibroblasts, indicating the responsible role of low-MW bioactive peptides in exerting the beneficial biological function. The anti-inflammatory activity of low-MW peptides was regulated through the inhibition of tumor necrosis factor-mediated nuclear factor κ-light-chain-enhancer of activated B cells activity. Our study demonstrated that both peptide composition and MW distribution play important roles in anti-inflammatory activity. The low-MW fractions prepared from ovomucin Alcalase hydrolysate may have potential applications for maintenance of dermal health and treatment of skin diseases.
authors.affiliation: Department of Agricultural, Food & Nutritional Science, University of Alberta, Edmonton, AB, Canada.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Agricultural, Food & Nutritional Science, University of Alberta, Edmonton, AB, Canada; Cardiovascular Research Centre, University of Alberta, Edmonton, AB, Canada.
authors.forename: Subhadeep
authors.initials: S
authors.lastname: Chakrabarti
authors.affiliation: College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China; Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, Hunan, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Fang
authors.affiliation: College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China; Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, Hunan, China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Department of Agricultural, Food & Nutritional Science, University of Alberta, Edmonton, AB, Canada; Cardiovascular Research Centre, University of Alberta, Edmonton, AB, Canada. Electronic address: jwu3@ualberta.ca.
authors.forename: Jianping
authors.initials: J
authors.lastname: Wu
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002478:Cells, Cultured; D004531:Eggs / Q000032:analysis; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D008970:Molecular Weight; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism; D010059:Ovomucin / Q000494:pharmacology*; D012867:Skin / Q000166:cytology; D013381:Subtilisins; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D010059:Ovomucin; D013381:Subtilisins
keywords: Dermal fibroblast; Desalting; Inflammation; NF-κB; Ovomucin hydrolysate; Ultrafiltration