title: [ADAR1 Knockout Inhibits Notch1-induced T-ALL in Mice].
abstract: OBJECTIVE
  To investigate the effect of ADAR1 on the occurrence and development of mouse T cell acute lymphoblastic leukemia (T-ALL).
  
  
  METHODS
  Lck-Cre; ADAR1lox/lox mice were generated through interbreeding. The lineage-cells of Lck-Cre; ADAR1lox/lox mice and the control were enriched respectively by the means of MACS, and the lin- cells were transfected with retrovirus carrying MSCV-ICN1-IRES-GFP fusion gene. Then the transfection efficiency was detected by the means of FACS, and the same number of GFP+ cells were transplanted into lethally irradiated recipient mice to observe the survival of mice in 2 recipient group after transplantation.
  
  
  RESULTS
  T cell-specific knockout ADAR1 mice were generated, and Notch1-induced T-ALL mouse model was established successfully. The leukemia with T-ALL characteristics occured in the mice of control group, but did not in the ADAR1 kmockout mice after transplantation.
  
  
  CONCLUSIONS
  ADAR1 plays a key role in the incidence and development of Notch1-induced T-ALL.
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020 China.|Co-First Authors: GAO Hui-Er and PENG Lu-Yun contributed equally to this work.
authors.forename: Hui-Er
authors.initials: HE
authors.lastname: Gao
authors.affiliation: Department of Laboratorial Examination, The Second Hospital of West China, Sichuan University, Chengdu 610041, Sichuan Province, China.|Co-First Authors: GAO Hui-Er and PENG Lu-Yun contributed equally to this work.
authors.forename: Lu-Yun
authors.initials: LY
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020 China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020 China.
authors.forename: Ying-Chi
authors.initials: YC
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020 China.
authors.forename: Tian-Yuan
authors.initials: TY
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020 China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020 China.
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020 China.
authors.forename: Tao
authors.initials: T
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020 China. E-mail: ydgao@ihcams.ac.cn.
authors.forename: Ying-Dai
authors.initials: YD
authors.lastname: Gao
mesh_terms: D000243:Adenosine Deaminase / Q000235:genetics*; D000818:Animals; D004195:Disease Models, Animal; D051379:Mice; D018345:Mice, Knockout; D054218:Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*; D051881:Receptor, Notch1 / Q000235:genetics*; D013601:T-Lymphocytes
chemical_list: C497762:Notch1 protein, mouse; D051881:Receptor, Notch1; C000599465:ADAR1 protein, mouse; D000243:Adenosine Deaminase