title: Protective effects of Astragalus polysaccharides against endothelial dysfunction in hypertrophic rats induced by isoproterenol.
abstract: Astragalus polysaccharide (APS) is an important bioactive component extracted from Chinese herb Astragalus membranaceus. It has been widely used in treatment of cardiovascular diseases. We have previously reported that APS could inhibit isoproterenol-induced cardiac hypertrophy. The present study was designed to evaluate the protective effect of APS on vascular endothelia in cardiac hypertrophy rats induced by isoproterenol (ISO). ISO (10mg×kg(-1)) was intraperitoneally injected once daily for 2weeks to induce cardiac hypertrophy. APS (400 and 800mg×kg(-1)) was intragastrically injected once daily along with ISO. The results showed that combination with APS significantly ameliorates the endothelial dysfunction while attenuates cardiac hypertrophy induced by ISO. We found that administration with APS could attenuate the increase in number of circulating endothelial cell (CEC). APS also decreases the superoxide anion generation and the protein expression of p65 and the levels of TNF-α and IL-6; while increases the cGMP levels, an activity marker for nitric oxide (NO) in aortas. In addition, APS improves the relaxation dysfunction in isolated aortic rings and increases the protein expression of IκBα and Cu/Zn-SOD in aortas. In conclusion, our results suggested that APS had a protective effect against endothelial dysfunction in hypertrophic rats induced by ISO. The underlining mechanisms may be contributed to the anti-inflammatory effects and the improvement of the imbalance between reactive oxygen species (ROS) and NO.
authors.affiliation: Key Laboratory of Cardiovascular and Cerebrovascular Drug Research of Liaoning Province, Drug Research Institute, Jinzhou Medical University, No. 40, Section 3, Songpo Road, Jinzhou 121001, PR China. Electronic address: 15204539981@163.com.
authors.forename: Ronghui
authors.initials: R
authors.lastname: Han
authors.affiliation: Key Laboratory of Cardiovascular and Cerebrovascular Drug Research of Liaoning Province, Drug Research Institute, Jinzhou Medical University, No. 40, Section 3, Songpo Road, Jinzhou 121001, PR China.
authors.forename: Futian
authors.initials: F
authors.lastname: Tang
authors.affiliation: Key Laboratory of Cardiovascular and Cerebrovascular Drug Research of Liaoning Province, Drug Research Institute, Jinzhou Medical University, No. 40, Section 3, Songpo Road, Jinzhou 121001, PR China.
authors.forename: Meili
authors.initials: M
authors.lastname: Lu
authors.affiliation: Key Laboratory of Cardiovascular and Cerebrovascular Drug Research of Liaoning Province, Drug Research Institute, Jinzhou Medical University, No. 40, Section 3, Songpo Road, Jinzhou 121001, PR China.
authors.forename: Chonghua
authors.initials: C
authors.lastname: Xu
authors.affiliation: Key Laboratory of Cardiovascular and Cerebrovascular Drug Research of Liaoning Province, Drug Research Institute, Jinzhou Medical University, No. 40, Section 3, Songpo Road, Jinzhou 121001, PR China.
authors.forename: Jin
authors.initials: J
authors.lastname: Hu
authors.affiliation: Key Laboratory of Cardiovascular and Cerebrovascular Drug Research of Liaoning Province, Drug Research Institute, Jinzhou Medical University, No. 40, Section 3, Songpo Road, Jinzhou 121001, PR China.
authors.forename: Meng
authors.initials: M
authors.lastname: Mei
authors.affiliation: Key Laboratory of Cardiovascular and Cerebrovascular Drug Research of Liaoning Province, Drug Research Institute, Jinzhou Medical University, No. 40, Section 3, Songpo Road, Jinzhou 121001, PR China. Electronic address: jyhxwang@163.com.
authors.forename: Hongxin
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D001011:Aorta / Q000187:drug effects* / Q000473:pathology; D027885:Astragalus propinquus / Q000276:immunology*; D006332:Cardiomegaly / Q000139:chemically induced / Q000188:drug therapy*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D007545:Isoproterenol; D008297:Male; D016328:NF-kappa B / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D009924:Organ Culture Techniques; D011134:Polysaccharides / Q000627:therapeutic use*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D000072105:Superoxide Dismutase-1 / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D018836:Inflammation Mediators; D015850:Interleukin-6; D016328:NF-kappa B; D011134:Polysaccharides; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D000072105:Superoxide Dismutase-1; D007545:Isoproterenol
keywords: Astragalus polysaccharide; Cardiac hypertrophy; Endothelial dysfunction; Isoproterenol; cGMP