title: Duplex Quantitative PCR Assay for Detection of Haemophilus influenzae That Distinguishes Fucose- and Protein D-Negative Strains.
abstract: We have developed a specific Haemophilus influenzae quantitative PCR (qPCR) that also identifies fucose-negative and protein D-negative strains. Analysis of 100 H. influenzae isolates, 28 Haemophilus haemolyticus isolates, and 14 other bacterial species revealed 100% sensitivity (95% confidence interval [CI], 96% to 100%) and 100% specificity (95% CI, 92% to 100%) for this assay. The evaluation of 80 clinical specimens demonstrated a strong correlation between semiquantitative culture and the qPCR (P < 0.001).
authors.affiliation: School of Paediatrics and Child Health, University of Western Australia, Perth, Australia.
authors.forename: Camilla
authors.initials: C
authors.lastname: de Gier
authors.affiliation: School of Paediatrics and Child Health, University of Western Australia, Perth, Australia Wesfarmers Centre for Vaccines and Infectious Diseases, Telethon Kids Institute, Perth, Australia.
authors.forename: Janessa L
authors.initials: JL
authors.lastname: Pickering
authors.affiliation: School of Paediatrics and Child Health, University of Western Australia, Perth, Australia Wesfarmers Centre for Vaccines and Infectious Diseases, Telethon Kids Institute, Perth, Australia Department of Paediatrics, Princess Margaret Hospital for Children, Subiaco, Australia.
authors.forename: Peter C
authors.initials: PC
authors.lastname: Richmond
authors.affiliation: School of Paediatrics and Child Health, University of Western Australia, Perth, Australia Wesfarmers Centre for Vaccines and Infectious Diseases, Telethon Kids Institute, Perth, Australia.
authors.forename: Ruth B
authors.initials: RB
authors.lastname: Thornton
authors.affiliation: School of Paediatrics and Child Health, University of Western Australia, Perth, Australia Wesfarmers Centre for Vaccines and Infectious Diseases, Telethon Kids Institute, Perth, Australia lea-ann.kirkham@uwa.edu.au.
authors.forename: Lea-Ann S
authors.initials: LA
authors.lastname: Kirkham
mesh_terms: D001426:Bacterial Proteins; D002352:Carrier Proteins; D005643:Fucose / Q000172:deficiency*; D006193:Haemophilus influenzae / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D007072:Immunoglobulin D / Q000172:deficiency*; D008074:Lipoproteins / Q000172:deficiency*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D007072:Immunoglobulin D; D008074:Lipoproteins; D005643:Fucose; C067989:glpQ protein, Haemophilus influenzae