title: Development and Validation of Stability Indicating Spectroscopic Method for Content Analysis of Ceftriaxone Sodium in Pharmaceuticals.
abstract: A simple, selective, and stability indicating spectroscopic method has been selected and validated for the assay of ceftriaxone sodium in the powder for injection dosage forms. Proposed method is based on the measurement of absorbance of ceftriaxone sodium in aqueous medium at 241 nm. The method obeys Beer's law in the range of 5-50 μg/mL with correlation coefficient of 0.9983. Apparent molar absorptivity and Sandell's sensitivity were found to be 2.046 × 10(3) L mol(-1) cm(-1) and 0.02732 μg/cm(2)/0.001 absorbance units. This study indicated that ceftriaxone sodium was degraded in acid medium and also underwent oxidative degradation. Percent relative standard deviation associated with all the validation parameters was less than 2, showing compliance with acceptance criteria of Q2 (R1), International Conference on Harmonization (2005) guidelines. Then the proposed method was successfully applied to the determination of ceftriaxone sodium in sterile preparation and results were comparable with reported methods.
authors.affiliation: Department of Pharmaceutical Analysis, The Erode College of Pharmacy, Erode, Tamil Nadu 638112, India.
authors.forename: Revathi
authors.initials: R
authors.lastname: Ethiraj
authors.affiliation: Department of Pharmaceutics, The Erode College of Pharmacy, Erode, Tamil Nadu 638112, India.
authors.forename: Ethiraj
authors.initials: E
authors.lastname: Thiruvengadam
authors.affiliation: Department of Pharmaceutical Analysis, The Erode College of Pharmacy, Erode, Tamil Nadu 638112, India.
authors.forename: Venkattapuram Saravanan
authors.initials: VS
authors.lastname: Sampath
authors.affiliation: Department of Pharmaceutical Analysis, The Erode College of Pharmacy, Erode, Tamil Nadu 638112, India.
authors.forename: Abdul
authors.initials: A
authors.lastname: Vahid
authors.affiliation: Department of Pharmaceutical Analysis, The Erode College of Pharmacy, Erode, Tamil Nadu 638112, India.
authors.forename: Jithin
authors.initials: J
authors.lastname: Raj