title: Peptidorhamnomannan negatively modulates the immune response in a scedosporiosis murine model.
abstract: In this study, we analyzed the impact of immunization with the peptidorhamnomannan (PRM) from the cell wall of the fungus Scedosporium (Lomentospora) prolificans in a murine model of invasive scedosporiosis. Immunization with PRM decreased the survival of mice infected with S. prolificans. Immunization of mice with PRM led to decreased secretion of pro-inflammatory cytokines and chemokines but did not affect the secretion of IL-10. Mice immunized with PRM showed an increase in IgG1 secretion, which is an immunoglobulin linked to a nonprotective response. Splenocytes isolated from mice infected with S. prolificans and immunized with PRM showed no differences in the percentages of Th17 cells and no increase in the frequency of the CD4(+)CD62L(Low) T cell population. PRM-immunized mice showed a significant increase in the percentage of Treg cells. In summary, our results indicated that immunization with PRM did not assist or improve the immunological response against S. prolificans infection. PRM exacerbated the infection process by reducing the inflammatory response, thereby facilitating colonization, virulence and dissemination by the fungus.
authors.affiliation: Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
authors.forename: Mariana I D S
authors.initials: MI
authors.lastname: Xisto
authors.affiliation: Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil liporagi@pharma.ufrj.br.
authors.forename: Livia Cristina
authors.initials: LC
authors.lastname: Liporagi-Lopes
authors.affiliation: Instituto de Ciências Biomédicas, Departamento de Microbiologia, Universidade de São Paulo (USP), São Paulo, Brazil.
authors.forename: Julián Esteban
authors.initials: JE
authors.lastname: Muñoz
authors.affiliation: Departamento de Microbiologia e Parasitologia, Instituto Biomédico, Universidade Federal do Estado do Rio de Janeiro (UNIRIO), Rio de Janeiro, Brazil.
authors.forename: Vera C B
authors.initials: VC
authors.lastname: Bittencourt
authors.affiliation: Instituto Biomédico, Departamento de Microbiologia e Parasitologia, Universidade Federal Fluminense (UFF), Niterói, Brasil.
authors.forename: Giulia M P
authors.initials: GM
authors.lastname: Santos
authors.affiliation: Instituto de Ciências Biomédicas, Departamento de Microbiologia, Universidade de São Paulo (USP), São Paulo, Brazil.
authors.forename: Lucas S
authors.initials: LS
authors.lastname: Dias
authors.affiliation: Campus de Xerém, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
authors.forename: Rodrigo T
authors.initials: RT
authors.lastname: Figueiredo
authors.affiliation: Instituto Biomédico, Departamento de Microbiologia e Parasitologia, Universidade Federal Fluminense (UFF), Niterói, Brasil.
authors.forename: Márcia R
authors.initials: MR
authors.lastname: Pinto
authors.affiliation: Instituto de Ciências Biomédicas, Departamento de Microbiologia, Universidade de São Paulo (USP), São Paulo, Brazil.
authors.forename: Carlos P
authors.initials: CP
authors.lastname: Taborda
authors.affiliation: Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil.
authors.forename: Eliana
authors.initials: E
authors.lastname: Barreto-Bergter
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D005657:Fungal Vaccines / Q000008:administration & dosage / Q000276:immunology; D006023:Glycoproteins / Q000378:metabolism*; D007074:Immunoglobulin G / Q000097:blood; D007166:Immunosuppressive Agents / Q000378:metabolism*; D008807:Mice, Inbred BALB C; D009181:Mycoses / Q000382:microbiology* / Q000473:pathology*; D021681:Scedosporium / Q000254:growth & development* / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
chemical_list: D005657:Fungal Vaccines; D006023:Glycoproteins; D007074:Immunoglobulin G; D007166:Immunosuppressive Agents; C107788:peptido-rhamnomannan
keywords: Scedosporium prolificans; immune response; immunization; peptidorhamnomannan