title: Synthetic phospholipids as specific substrates for plasma endothelial lipase.
abstract: We designed and prepared synthetic phospholipids that generate lyso-phosphatidylcholine products with a unique mass for convenient detection by LC-MS in complex biological matrices. We demonstrated that compound 4, formulated either as a Triton X-100 emulsion or incorporated in synthetic HDL particles can serve as a substrate for plasma EL with useful specificity.
authors.affiliation: Global Discovery Chemistry, Novartis Institutes for BioMedical Research, 250 Massachusetts Avenue, Cambridge, MA 02139, United States. Electronic address: julien.papillon@novartis.com.
authors.forename: Julien P N
authors.initials: JP
authors.lastname: Papillon
authors.affiliation: Cardiovascular and Metabolism, Novartis Institutes for BioMedical Research, 100 Technology Square, Cambridge, MA 02139, United States. Electronic address: meihui.pan@novartis.com.
authors.forename: Meihui
authors.initials: M
authors.lastname: Pan
authors.affiliation: Cardiovascular and Metabolism, Novartis Institutes for BioMedical Research, 100 Technology Square, Cambridge, MA 02139, United States.
authors.forename: Margaret E
authors.initials: ME
authors.lastname: Brousseau
authors.affiliation: Cardiovascular and Metabolism, Novartis Institutes for BioMedical Research, 100 Technology Square, Cambridge, MA 02139, United States.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Gilchrist
authors.affiliation: Global Discovery Chemistry, Novartis Institutes for BioMedical Research, 250 Massachusetts Avenue, Cambridge, MA 02139, United States.
authors.forename: Changgang
authors.initials: C
authors.lastname: Lou
authors.affiliation: Global Discovery Chemistry, Novartis Institutes for BioMedical Research, 250 Massachusetts Avenue, Cambridge, MA 02139, United States.
authors.forename: Alok K
authors.initials: AK
authors.lastname: Singh
authors.affiliation: Cardiovascular and Metabolism, Novartis Institutes for BioMedical Research, 100 Technology Square, Cambridge, MA 02139, United States.
authors.forename: Todd
authors.initials: T
authors.lastname: Stawicki
authors.affiliation: Cardiovascular and Metabolism, Novartis Institutes for BioMedical Research, 100 Technology Square, Cambridge, MA 02139, United States.
authors.forename: James E
authors.initials: JE
authors.lastname: Thompson
mesh_terms: D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008049:Lipase / Q000037:antagonists & inhibitors* / Q000097:blood / Q000378:metabolism; D015394:Molecular Structure; D010743:Phospholipids / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship
chemical_list: D004791:Enzyme Inhibitors; D010743:Phospholipids; C410382:LIPG protein, human; D008049:Lipase
keywords: Endothelial lipase; HPLC–MS/MS; Lipid analysis; Lipid mass spectrometry; Plasma assay; Synthetic phospholipids; Tandem mass spectrometry