title: Effect of endophytic Bacillus cereus ERBP inoculation into non-native host: Potentials and challenges for airborne formaldehyde removal.
abstract: Phytoremediation could be a cost-effective, environmentally friendly approach for the treatment of indoor air. However, some drawbacks still dispute the expediency of phytotechnology. Our objectives were to investigate the competency of plant growth-promoting (PGP) endophytic Bacillus cereus ERBP (endophyte root blue pea), isolated from the root of Clitoria ternatea, to colonize and stabilize within Zamioculcas zamiifolia and Euphorbia milii as non-native hosts without causing any disease or stress symptoms. Moreover, the impact of B. cereus ERBP on the natural shoot endophytic community and for the airborne formaldehyde removal capability of non-native hosts was assessed. Non-native Z. zamiifolia was effectively inoculated with B. cereus ERBP through soil as the most efficient method of endophyte inoculation. Denaturing gradient gel electrophoresis profiling of the shoot endophytic community verified the colonization and stability of B. cereus ERBP within its non-native host during a 20-d fumigation period without interfering with the natural shoot endophytic diversity of Z. zamiifolia. B. cereus ERBP conferred full protection to its non-native host against formaldehyde phytotoxicity and enhanced airborne formaldehyde removal of Z. zamiifolia whereas non-inoculated plants suffered from formaldehyde phytotoxicity because their natural shoot endophytic community was detrimentally affected by formaldehyde. In contrast, B. cereus ERBP inoculation into non-native E. milii deteriorated airborne formaldehyde removal of the non-native host (compared to a non-inoculated one) as B. cereus ERBP interfered with natural shoot endophytic community of E. milii, which caused stress symptoms and stimulated ethylene biosynthesis. Non-native host inoculation with PGP B. cereus ERBP could bear potentials and challenges for airborne formaldehyde removal.
authors.affiliation: School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkok, 10150, Thailand.
authors.forename: Gholamreza
authors.initials: G
authors.lastname: Khaksar
authors.affiliation: Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi, Bangkok, 10150, Thailand.
authors.forename: Chairat
authors.initials: C
authors.lastname: Treesubsuntorn
authors.affiliation: School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkok, 10150, Thailand. Electronic address: paitip.thi@kmutt.ac.th.
authors.forename: Paitip
authors.initials: P
authors.lastname: Thiravetyan
mesh_terms: D000393:Air Pollutants / Q000302:isolation & purification*; D029064:Araceae / Q000187:drug effects / Q000235:genetics / Q000382:microbiology*; D001409:Bacillus cereus / Q000187:drug effects / Q000502:physiology*; D001673:Biodegradation, Environmental / Q000187:drug effects; D058645:Denaturing Gradient Gel Electrophoresis; D060026:Endophytes / Q000187:drug effects / Q000502:physiology*; D028481:Euphorbia / Q000187:drug effects / Q000235:genetics / Q000382:microbiology*; D005557:Formaldehyde / Q000302:isolation & purification* / Q000633:toxicity; D017343:Genes, Plant; D007210:Indoleacetic Acids / Q000378:metabolism; D018520:Plant Shoots / Q000187:drug effects / Q000235:genetics; D054046:Plant Stomata / Q000187:drug effects / Q000502:physiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics
chemical_list: D000393:Air Pollutants; D007210:Indoleacetic Acids; D012336:RNA, Ribosomal, 16S; D005557:Formaldehyde
keywords: Airborne formaldehyde; Bacillus cereus; Euphorbia milii; Non-native host; Phytoremediation; Plant-endophyte interactions; Zamioculcas zamiifolia