title: [Frequency Distribution Features of Innate-like Lymphocytes in Peripheral Blood of Normal Adults].
abstract: OBJECTIVE
  To investigate the frequency distribution features of innate-like lymphocytes (iNKT cells, γΔT cells and B1 cells) in peripheral blood of normal adults.
  
  
  METHODS
  The flow cytometry with 6 fluorescence staining was used to detect the percentages of iNKT lymphocytes, γΔT lymphocytes, B1 lymphocytes and adaptive T lymphocyte, B2 lymphocytes in peripheral blood lymphocytes of 50 normal adults. The difference and correlation between these lymphocyte subsets were analyzed by statistical software.
  
  
  RESULTS
  The percentage of iNKT cells in peripheral blood of 50 normal adults was 0.18% (0.01%-2.01%), the percentage of γΔT cells was 4.90% (1.45%-20.14%), the percentage of B1 lymphocytes was 1.62% (0.20%-3.77%), the percentage of adaptive T cells was 63.52% (33.20%-83.22%), the percentage of B2 cells was 6.64% (3.07%-13.80%). B1 and B2 were two subsets of B lymphocyte, the percentage of B2 in B lymphocyte was 81.43% (57.90%-94.12%) and more than that of B1 lymphocyte; the percentage of B1 lymphocytes was 17.28% (5.28%-41.13%). In T lymphocyte group the percentage of iNKT cell was 0.32% (0.01%-3.6%), the percentages of γΔT cells and adaptive T cells were 7.55% (3.04%-27.66%) and 91.98% (72.22%-96.86%) respectively. Spearman correlation analysis was used to analyze the correlation between the percentages of several lymphocyte subsets. There was a positive correlation between iNK T cells and γΔT cells, γΔT cells and adaptive T cells, B1 cells and B2 cells (r=0.39, P=0.0056; r=0.6028, P<0.0001; r=0.4791, P=0.0004). It was also found that the percentage of iNKT cells in female peripheral blood lymphocytes was 0.29% (0.06%-2.01%), and significantly higher than that in male peripheral blood lymphocytes 0.12% (0.01%-1.37%) (P<0.05).
  
  
  CONCLUSIONS
  The percentages of γΔT cells, B1 cells and iNKT cells in peripheral blood lymphocytes of normal adults are significantly lower than that of adaptive lymphocytes, and their contents in peripheral blood decrease in turn. There are no sex differences in the percentages of these lymphocyte subsets except iNKT cells.
authors.affiliation: Department of Immunology, Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, Bengbu 233000, Anhei Province, China.
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Yao
authors.affiliation: Department of Immunology, Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, Bengbu 233000, Anhei Province, China.
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Wei
authors.affiliation: 2012 Grade of Medical Laboratory specialty, Bengbu Medical College, Bengbu 233000, Anhei Province, China.
authors.forename: Lu-Lu
authors.initials: LL
authors.lastname: Guo
authors.affiliation: 2012 Grade of Medical Laboratory specialty, Bengbu Medical College, Bengbu 233000, Anhei Province, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Shan
authors.affiliation: Department of Immunology, Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, Bengbu 233000, Anhei Province, China. E-mail: bb_bqli@163.com.
authors.forename: Bai-Qing
authors.initials: BQ
authors.lastname: Li
mesh_terms: D000328:Adult; D001402:B-Lymphocytes / Q000166:cytology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D055611:Natural Killer T-Cells / Q000166:cytology*; D016176:T-Lymphocyte Subsets / Q000166:cytology*