title: Characterization of unrelated clinical Legionella pneumophila isolates in Catalonia by monoclonal subgrouping and sequence-based typing.
abstract: To characterize the genetic diversity of unrelated Legionella pneumophila clinical isolates in Catalonia and compare with other European regions.
  
  
  
  95 unrelated isolates were analyzed using monoclonal antibodies and sequence-based typing, 1989-2013.
  
  
  
  The isolates showed a high diversity (IOD 0.964) with a predominance of some profiles (ST37-Phialdelphia, ST23-Philadelphia and ST1-OLDA). All regions had predominant sequence types (STs) that differed between regions, and only 3% of STs were shared between the three regions.
  
  
  
  L. pneumophila clinical isolates from Catalonia presented a high diversity and can be used in epidemiological surveillance studies. The heterogeneous predominance of STs between European regions suggested a relationship between geographical distribution and virulence of some STs.
authors.affiliation: Infectious Diseases Unit, Fundació Institut d'Investigació Germans Trias i Pujol, Hospital Germans Trias i Pujol, Badalona, Spain E-08916.|Grupo de estudio de la Legionelosis, Autonomous University of Barcelona, Cerdanyola, Spain E-08913.|Ciber de Enfermedades Respiratorias, Madrid, Spain E-28029.
authors.forename: Marian
authors.initials: M
authors.lastname: Garcia-Nuñez
authors.affiliation: Infectious Diseases Unit, Fundació Institut d'Investigació Germans Trias i Pujol, Hospital Germans Trias i Pujol, Badalona, Spain E-08916.|Grupo de estudio de la Legionelosis, Autonomous University of Barcelona, Cerdanyola, Spain E-08913.|Ciber de Enfermedades Respiratorias, Madrid, Spain E-28029.
authors.forename: Sara
authors.initials: S
authors.lastname: Quero
authors.affiliation: Infectious Diseases Unit, Fundació Institut d'Investigació Germans Trias i Pujol, Hospital Germans Trias i Pujol, Badalona, Spain E-08916.|Grupo de estudio de la Legionelosis, Autonomous University of Barcelona, Cerdanyola, Spain E-08913.|Ciber de Enfermedades Respiratorias, Madrid, Spain E-28029.
authors.forename: Maria Lluisa
authors.initials: ML
authors.lastname: Pedro-Botet
authors.affiliation: Epidemiological Surveillance, Publich Health Agency of Catalonia, Barcelona, Spain E-08005.
authors.forename: Irene
authors.initials: I
authors.lastname: Barrabeig
authors.affiliation: Epidemiological Surveillance, Publich Health Agency of Catalonia, Barcelona, Spain E-08005.
authors.forename: Josep
authors.initials: J
authors.lastname: Avarez
authors.affiliation: Infectious Diseases Unit, Fundació Institut d'Investigació Germans Trias i Pujol, Hospital Germans Trias i Pujol, Badalona, Spain E-08916.
authors.forename: Irene
authors.initials: I
authors.lastname: Campoy
authors.affiliation: Epidemiological Surveillance, Publich Health Agency of Catalonia, Barcelona, Spain E-08005.
authors.forename: Maria Rosa
authors.initials: MR
authors.lastname: Sala
authors.affiliation: Infectious Diseases Unit, Fundació Institut d'Investigació Germans Trias i Pujol, Hospital Germans Trias i Pujol, Badalona, Spain E-08916.|Grupo de estudio de la Legionelosis, Autonomous University of Barcelona, Cerdanyola, Spain E-08913.|Ciber de Enfermedades Respiratorias, Madrid, Spain E-28029.
authors.forename: Noemí
authors.initials: N
authors.lastname: Parraga-Niño
authors.affiliation: Epidemiological Surveillance, Publich Health Agency of Catalonia, Barcelona, Spain E-08005.
authors.forename: Sofia
authors.initials: S
authors.lastname: Minguell
authors.affiliation: Epidemiological Surveillance, Publich Health Agency of Catalonia, Barcelona, Spain E-08005.|Ciber de Salud Publica, Madrid, Spain, E-28029.
authors.forename: Joan
authors.initials: J
authors.lastname: Caylà
authors.affiliation: Infectious Diseases Unit, Fundació Institut d'Investigació Germans Trias i Pujol, Hospital Germans Trias i Pujol, Badalona, Spain E-08916.|Grupo de estudio de la Legionelosis, Autonomous University of Barcelona, Cerdanyola, Spain E-08913.|Ciber de Enfermedades Respiratorias, Madrid, Spain E-28029.
authors.forename: Lourdes
authors.initials: L
authors.lastname: Mateu
authors.affiliation: Infectious Diseases Unit, Fundació Institut d'Investigació Germans Trias i Pujol, Hospital Germans Trias i Pujol, Badalona, Spain E-08916.|Grupo de estudio de la Legionelosis, Autonomous University of Barcelona, Cerdanyola, Spain E-08913.|Ciber de Enfermedades Respiratorias, Madrid, Spain E-28029.
authors.forename: Miquel
authors.initials: M
authors.lastname: Sabria
mesh_terms: D000911:Antibodies, Monoclonal; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000302:isolation & purification; D005060:Europe / Q000453:epidemiology; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D016952:Legionella pneumophila / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification*; D007877:Legionnaires' Disease / Q000453:epidemiology / Q000382:microbiology*; D058889:Molecular Typing / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods; D065288:Serogroup; D012703:Serotyping
chemical_list: D000911:Antibodies, Monoclonal; D004269:DNA, Bacterial
keywords: Legionella; clinical isolates; monoclonal antibody; sequence-based typing