title: Morphologic and Molecular Characterization of a Demodex (Acari: Demodicidae) Species from White-Tailed Deer (Odocoileus virginianus).
abstract: Demodex mites, although usually nonpathogenic, can cause a wide range of dermatological lesions ranging from mild skin irritation and alopecia to severe furunculosis. Recently, a case of demodicosis from a white-tailed deer (Odocoileus virginianus) revealed a Demodex species morphologically distinct from Demodex odocoilei. All life cycle stages were considerably larger than D. odocoilei and although similar in size to D. kutzeri and D. acutipes from European cervids, numerous morphometrics distinguished the four species. Adult males and females were 209.1 ± 13.1 and 225.5 ± 13.4 μm in length, respectively. Ova, larva, and nymphs measured 65.1 ± 4.1, 124.9 ± 11.6, and 205.1 ± 19.4 μm in length, respectively. For phylogenetic analyses, a portion of the 18S rRNA gene was amplified and sequenced from samples of the WTD Demodex sp., two Demodex samples from domestic dogs, and Demodex ursi from a black bear. Phylogenetic analyses indicated that the WTD Demodex was most similar to D. musculi from laboratory mice. A partial sequence from D. ursi was identical to the WTD Demodex sequence; however, these two species can be differentiated morphologically. This paper describes a second Demodex species from white-tailed deer and indicates that 18S rRNA is useful for phylogenetic analysis of most Demodex species, but two morphologically distinct species had identical partial sequences. Additional gene targets should be investigated for phylogenetic and parasite-host association studies.
authors.affiliation: Southeastern Cooperative Wildlife Disease Study, Department of Population Health, The University of Georgia College of Veterinary Medicine, Wildlife Health Building, Athens, GA 30602, USA; Warnell School of Forestry and Natural Resources, The University of Georgia, Athens, GA 30602, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Yabsley
authors.affiliation: Southeastern Cooperative Wildlife Disease Study, Department of Population Health, The University of Georgia College of Veterinary Medicine, Wildlife Health Building, Athens, GA 30602, USA.
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Clay
authors.affiliation: Southeastern Cooperative Wildlife Disease Study, Department of Population Health, The University of Georgia College of Veterinary Medicine, Wildlife Health Building, Athens, GA 30602, USA; Division of Migratory Bird Management, U.S Fish & Wildlife Service, Laurel, MD 20708, USA.
authors.forename: Samantha E J
authors.initials: SE
authors.lastname: Gibbs
authors.affiliation: Florida Fish and Wildlife Conservation Commission, Gainesville, FL 32653, USA.
authors.forename: Mark W
authors.initials: MW
authors.lastname: Cunningham
authors.affiliation: Department of Small Animal Medicine and Surgery, The University of Georgia College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA; Massachusetts Veterinary Referral Hospital, Woburn, MA 01801, USA.
authors.forename: Michaela G
authors.initials: MG
authors.lastname: Austel