title: Fermentative lactic acid production from coffee pulp hydrolysate using Bacillus coagulans at laboratory and pilot scales.
abstract: In this study, the lignocellulosic residue coffee pulp was used as carbon source in fermentative l(+)-lactic acid production using Bacillus coagulans. After thermo-chemical treatment at 121°C for 30min in presence of 0.18molL(-1) H2SO4 and following an enzymatic digestion using Accellerase 1500 carbon-rich hydrolysates were obtained. Two different coffee pulp materials with comparable biomass composition were used, but sugar concentrations in hydrolysates showed variations. The primary sugars were (gL(-1)) glucose (20-30), xylose (15-25), sucrose (5-11) and arabinose (0.7-10). Fermentations were carried out at laboratory (2L) and pilot (50L) scales in presence of 10gL(-1) yeast extract. At pilot scale carbon utilization and lactic acid yield per gram of sugar consumed were 94.65% and 0.78gg(-1), respectively. The productivity was 4.02gL(-1)h(-1). Downstream processing resulted in a pure formulation containing 937gL(-1)l(+)-lactic acid with an optical purity of 99.7%.
authors.affiliation: Leibniz Institute for Agricultural Engineering Potsdam-Bornim, Max-Eyth-Allee 100, 14469 Potsdam, Germany; Sustainable Chemistry, Institute of Sustainable Environmental Chemistry, Leuphana University of Lüneburg, Scharnhorststr. 1, C13, 21335 Lüneburg, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Pleissner
authors.affiliation: Leibniz Institute for Agricultural Engineering Potsdam-Bornim, Max-Eyth-Allee 100, 14469 Potsdam, Germany.
authors.forename: Anna-Katrin
authors.initials: AK
authors.lastname: Neu
authors.affiliation: Leibniz Institute for Agricultural Engineering Potsdam-Bornim, Max-Eyth-Allee 100, 14469 Potsdam, Germany.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Mehlmann
authors.affiliation: Leibniz Institute for Agricultural Engineering Potsdam-Bornim, Max-Eyth-Allee 100, 14469 Potsdam, Germany.
authors.forename: Roland
authors.initials: R
authors.lastname: Schneider
authors.affiliation: Cenicafé, National Coffee Research Center, Sede Planalto, km. 4 via Chinchiná-Manizales, Manizales (Caldas), Colombia.
authors.forename: Gloria Inés
authors.initials: GI
authors.lastname: Puerta-Quintero
authors.affiliation: Leibniz Institute for Agricultural Engineering Potsdam-Bornim, Max-Eyth-Allee 100, 14469 Potsdam, Germany. Electronic address: jvenus@atb-potsdam.de.
authors.forename: Joachim
authors.initials: J
authors.lastname: Venus
mesh_terms: D001089:Arabinose / Q000737:chemistry; D000070000:Bacillus coagulans / Q000378:metabolism*; D018533:Biomass; D003069:Coffee / Q000737:chemistry*; D005285:Fermentation; D005947:Glucose / Q000737:chemistry; D006868:Hydrolysis; D019344:Lactic Acid / Q000096:biosynthesis*; D012037:Refuse Disposal; D062611:Solid Waste; D014867:Water / Q000737:chemistry; D014994:Xylose
chemical_list: D003069:Coffee; D062611:Solid Waste; D014867:Water; D019344:Lactic Acid; D014994:Xylose; D001089:Arabinose; D005947:Glucose
keywords: Agricultural residue utilization; Bacillus coagulans; Coffee pulp; Renewable resources