title: [Emodin inhibits the proliferation, transdifferentiation and collagen synthesis of pulmonary fibroblasts].
abstract: Objective To explore the effect of emodin on the proliferation, differentiation into myofibroblasts and collagen synthesis of pulmonary fibroblasts and the underlying mechanisms. Methods Human pulmonary fibroblasts MRC-5 were cultured in vitro, then the cells were inoculated with dimethyl sulfoxide (DMSO) added with 0, 10, 20, 40, 80 and 160 μmol/L emodin for 24, 48 and 72 hours. Inhibitory rate of cell proliferation was analyzed by MTT assay. Based on the results of cell proliferation experiment, MRC-5 cells were treated with DMSO (control group) and 40, 80 μmol/L emodin (in DMSO) for 48 hours. Fluorescence real-time quantitative PCR was then used to measure the mRNA expressions of α-smooth muscle actin (α-SMA), transforming growth factor-β1 (TGF-β1), a disintegrin-like and metalloproteinase with thrombospondin type 1 motif (ADAMTS-1), collagen type 1 (Col1) and collagen type 3 (Col3). The protein expressions of the above mentioned factors were also measured by Western blotting. Results In a concentration- and time-dependent manner, emodin inhibited MRC-5 cell proliferation. After 48 hours of co-culture, in comparison with control group, the mRNA and protein expression levels of α-SMA, TGF-β1, Col1 and Col3 significantly decreased, while the mRNA and protein expression levels of ADAMTS-1 significantly increased in 40 and 80 μmol/L emodin-treated groups. Moreover, in comparison with 40 μmol/L emodin-treated group, the mRNA and protein expressions of α-SMA, TGF-β1, Col1 and Col3 were significantly downregulated, but ADAMTS-1 mRNA and protein expressions were significantly upregulated in 80 μmol/L emodin-treated group. Conclusion Emodin can block pulmonary fibroblast proliferation and differentiation into myofibroblasts, and reduce the synthesis of Col1 and Col3 by inhibiting TGF-β1/ADAMTS-1 signaling pathway.
authors.affiliation: School of Clinical Medicine, Hunan University of Medicine, Huaihua 418000; Department of Respiration, First Affiliated Hospital, Hunan University of Medicine, Huaihua 418000, China.
authors.forename: Lijing
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Respiration, First Affiliated Hospital, Hunan University of Medicine, Huaihua 418000, China. *Corresponding author, E-mail: 1976841746@qq.com.
authors.forename: Huiming
authors.initials: H
authors.lastname: Yin
authors.affiliation: Department of Respiration, Huaihua First People's Hospital, Huaihua 418000, China.
authors.forename: Jianbin
authors.initials: J
authors.lastname: He
authors.affiliation: Department of Respiration, Huaihua First People's Hospital, Huaihua 418000, China.
authors.forename: Maofeng
authors.initials: M
authors.lastname: Xie
authors.affiliation: Department of Respiration, First Affiliated Hospital, University of South China, Hengyang 421001, China.
authors.forename: Zaiyan
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Respiration, First Affiliated Hospital, University of South China, Hengyang 421001, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Xiao
mesh_terms: D000071097:ADAMTS1 Protein / Q000235:genetics / Q000378:metabolism; D000199:Actins / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects* / Q000235:genetics; D054338:Cell Transdifferentiation / Q000187:drug effects* / Q000235:genetics; D003094:Collagen / Q000096:biosynthesis* / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D004642:Emodin / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D008168:Lung / Q000166:cytology; D009130:Muscle, Smooth / Q000378:metabolism; D058628:Myofibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism
chemical_list: D000199:Actins; D053773:Transforming Growth Factor beta1; D003094:Collagen; D000071097:ADAMTS1 Protein; D004642:Emodin