title: Relationships between rDNA, Nop1 and Sir complex in biotechnologically relevant distillery yeasts.
abstract: Distillery yeasts are poorly characterized physiological group among the Saccharomyces sensu stricto complex. As industrial yeasts are under constant environmental stress during fermentation processes and the nucleolus is a stress sensor, in the present study, nucleolus-related parameters were evaluated in 22 commercially available distillery yeast strains. Distillery yeasts were found to be a heterogeneous group with a variable content and length of rDNA and degree of nucleolus fragmentation. The levels of rDNA were negatively correlated with Nop1 (r = -0.59, p = 0.0038). Moreover, the protein levels of Sir transcriptional silencing complex and longevity regulators, namely Sir1, Sir2, Sir3 and Fob1, were studied and negative correlations between Sir2 and Nop1 (r = -0.45, p = 0.0332), and between Sir2 and Fob1 (r = -0.49, p = 0.0211) were revealed. In general, S. paradoxus group of distillery yeasts with higher rDNA pools and Sir2 level than S. bayanus group was found to be more tolerant to fermentation-associated stress stimuli, namely mild cold/heat stresses and KCl treatment. We postulate that rDNA state may be considered as a novel factor that may modulate a biotechnological process.
authors.affiliation: Department of Genetics, University of Rzeszow, Rejtana 16C, 35-959, Rzeszow, Poland.
authors.forename: Jagoda
authors.initials: J
authors.lastname: Adamczyk
authors.affiliation: Department of Genetics, University of Rzeszow, Rejtana 16C, 35-959, Rzeszow, Poland.|Postgraduate School of Molecular Medicine, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Deregowska
authors.affiliation: Department of Genetics, University of Rzeszow, Rejtana 16C, 35-959, Rzeszow, Poland.
authors.forename: Leszek
authors.initials: L
authors.lastname: Potocki
authors.affiliation: Department of Genetics, University of Rzeszow, Rejtana 16C, 35-959, Rzeszow, Poland.
authors.forename: Ewelina
authors.initials: E
authors.lastname: Kuna
authors.affiliation: Department of Genetics, University of Rzeszow, Rejtana 16C, 35-959, Rzeszow, Poland.
authors.forename: Jakub
authors.initials: J
authors.lastname: Kaplan
authors.affiliation: Department of Genetics, University of Rzeszow, Rejtana 16C, 35-959, Rzeszow, Poland.
authors.forename: Sylwia
authors.initials: S
authors.lastname: Pabian
authors.affiliation: Department of Genetics, University of Rzeszow, Rejtana 16C, 35-959, Rzeszow, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Kwiatkowska
authors.affiliation: Department of Biochemistry and Cell Biology, University of Rzeszow, Zelwerowicza 4, 35-601, Rzeszow, Poland. alewinska@o2.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Lewinska
authors.affiliation: Department of Genetics, University of Rzeszow, Rejtana 16C, 35-959, Rzeszow, Poland. mawnuk@gmail.com.
authors.forename: Maciej
authors.initials: M
authors.lastname: Wnuk
mesh_terms: D000434:Alcoholic Beverages / Q000382:microbiology*; D004275:DNA, Ribosomal / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics*; D009697:Nucleolus Organizer Region / Q000235:genetics; D020718:Ribonucleoproteins, Small Nucleolar / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae / Q000235:genetics*; D056565:Sirtuin 2 / Q000235:genetics*
chemical_list: D004275:DNA, Ribosomal; D004268:DNA-Binding Proteins; C105579:FOB1 protein, S cerevisiae; C064793:NOP1 protein, S cerevisiae; D009687:Nuclear Proteins; D020718:Ribonucleoproteins, Small Nucleolar; D029701:Saccharomyces cerevisiae Proteins; D038281:Silent Information Regulator Proteins, Saccharomyces cerevisiae; D056565:Sirtuin 2
keywords: Distillery yeasts; Nop1; Nucleolus; Sirtuins; rDNA