title: Alteration of Phospholipids during the Mitophagic Process in Lung Cancer Cells.
abstract: Matrix assisted laser desorption ionization (MALDI)-time of flight/mass spectrometry (TOF/MS) was applied to investigate alterations in phospholipids in mitophagic cancer cells. Several phospholipids, including phosphatidylcholines (PCs), sphingomyelins (SMs), and phosphatidylinositols (PIs), were successfully analyzed in control and mitophagy-induced H460 cells in the positive and negative ion modes. Principal component analysis was applied to differentiate the two groups. The upregulated and downregulated phospholipid species in the mitophagic cells were also represented in a heatmap. In the volcano plot (fold change > 1.3 andpvalue < 0.01), individual species of seven PCs, two SMs, and three PIs were selected as differentially regulated phospholipids. In particular, almost all the molecular species of PC, SM, and PI were downregulated in the mitophagic cells. Quantification of these lipids indicated that mitophagy induces altered metabolism of phospholipids. Therefore, phospholipid alterations during the mitophagic process of lung cancer cells were well characterized by MALDI-TOF/MS.
authors.affiliation: Department of Applied Chemistry, The Institute of Natural Science, College of Applied Science, Kyung Hee University, Yongin 17104, Republic of Korea.
authors.forename: Jae Won
authors.initials: JW
authors.lastname: Lee
authors.affiliation: Department of Applied Chemistry, The Institute of Natural Science, College of Applied Science, Kyung Hee University, Yongin 17104, Republic of Korea.
authors.forename: Kyung Mi
authors.initials: KM
authors.lastname: Cho
authors.affiliation: Department of Applied Chemistry, The Institute of Natural Science, College of Applied Science, Kyung Hee University, Yongin 17104, Republic of Korea.
authors.forename: Jae Hun
authors.initials: JH
authors.lastname: Jung
authors.affiliation: Department of Pharmacology, Metabolic Diseases and Cell Signaling Laboratory, Research Institute for Medical Sciences, College of Medicine, Chungnam National University, Daejeon 35245, Republic of Korea.
authors.forename: Quangdon
authors.initials: Q
authors.lastname: Tran
authors.affiliation: Department of Emergency Medicine, School of Medicine, Kyung Hee University, Seoul 05278, Republic of Korea.
authors.forename: Woong
authors.initials: W
authors.lastname: Jung
authors.affiliation: Department of Pharmacology, Metabolic Diseases and Cell Signaling Laboratory, Research Institute for Medical Sciences, College of Medicine, Chungnam National University, Daejeon 35245, Republic of Korea.
authors.forename: Jongsun
authors.initials: J
authors.lastname: Park
authors.affiliation: Department of Applied Chemistry, The Institute of Natural Science, College of Applied Science, Kyung Hee University, Yongin 17104, Republic of Korea.
authors.forename: Kwang Pyo
authors.initials: KP
authors.lastname: Kim
mesh_terms: D045744:Cell Line, Tumor; D016000:Cluster Analysis; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism*; D063306:Mitophagy / Q000502:physiology*; D010743:Phospholipids / Q000032:analysis* / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D010743:Phospholipids
keywords: MALDI; mass spectrometry; mitophagy; phospholipids