title: Proteomic analysis of cerebrospinal fluid for relapsing-remitting multiple sclerosis and clinically isolated syndrome.
abstract: Early diagnosis and treatment of multiple sclerosis (MS) in the initial stages of the disease can significantly retard its progression. The aim of the present study was to identify changes in the cerebrospinal fluid proteome in patients with relapsing-remitting MS and clinically isolated MS syndrome who are at high risk of developing MS (case group) compared to healthy population (control) in order to identify potential new markers, which could ultimately aid in early diagnosis of MS. The protein concentrations of each of the 11 case and 15 control samples were determined using a bicinchoninic acid assay. Nanoscale liquid chromatography coupled with tandem mass spectrometry was used for protein identification. Proteomics data were processed using the Perseus software suite and R. The results were filtered using the Benjamini-Hochberg procedure for the false discovery rate (FDR) correction (FDR<0.05). The results showed that, 26 proteins were significantly dysregulated in case samples compared to the controls. Nine proteins were found to be significantly less abundant in case samples, while the abundance of 17 proteins was significantly increased in case samples compared to controls. Three of the proteins were previously linked to RR MS, including immunoglobulin (Ig) γ-1 chain C region, Ig heavy chain V-III region BRO and Ig κ chain C region. Three proteins that were uniquely expressed in patients with RR MS were identified and these proteins may serve as prognostic biomarkers for identifying patients with a high risk of developing RR MS.
authors.affiliation: Department of Neurology, Faculty of Medicine and University Hospital Hradec Králové, Charles University in Prague, CZ-500 05 Hradec Králové, Czech Republic.
authors.forename: Zbyšek
authors.initials: Z
authors.lastname: Pavelek
authors.affiliation: Department of Neurology, Faculty of Medicine and University Hospital Hradec Králové, Charles University in Prague, CZ-500 05 Hradec Králové, Czech Republic.
authors.forename: Oldřich
authors.initials: O
authors.lastname: Vyšata
authors.affiliation: Biomedical Research Center, University Hospital Hradec Králové, CZ-500 05 Hradec Králové, Czech Republic.
authors.forename: Vojtěch
authors.initials: V
authors.lastname: Tambor
authors.affiliation: Biomedical Research Center, University Hospital Hradec Králové, CZ-500 05 Hradec Králové, Czech Republic.
authors.forename: Kristýna
authors.initials: K
authors.lastname: Pimková
authors.affiliation: Biomedical Research Center, University Hospital Hradec Králové, CZ-500 05 Hradec Králové, Czech Republic.
authors.forename: Dai Long
authors.initials: DL
authors.lastname: Vu
authors.affiliation: Biomedical Research Center, University Hospital Hradec Králové, CZ-500 05 Hradec Králové, Czech Republic.
authors.forename: Kamil
authors.initials: K
authors.lastname: Kuča
authors.affiliation: Department of Neurology, Faculty of Medicine and University Hospital Brno, CZ-639 00 Brno, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Šťourač
authors.affiliation: Department of Neurology, Faculty of Medicine and University Hospital Hradec Králové, Charles University in Prague, CZ-500 05 Hradec Králové, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Vališ
keywords: biomarkers; cerebrospinal fluid; clinically isolated syndrome; multiple sclerosis; proteins; proteomic analysis