title: Multi-Elemental Profiling of Tibial and Maxillary Trabecular Bone in Ovariectomised Rats.
abstract: Atomic minerals are the smallest components of bone and the content of Ca, being the most abundant mineral in bone, correlates strongly with the risk of osteoporosis. Postmenopausal women have a far greater risk of suffering from OP due to low Ca concentrations in their bones and this is associated with low bone mass and higher bone fracture rates. However, bone strength is determined not only by Ca level, but also a number of metallic and non-metallic elements in bone. Thus, in this study, the difference of metallic and non-metallic elements in ovariectomy-induced osteoporosis tibial and maxillary trabecular bone was investigated in comparison with sham operated normal bone by laser ablation inductively-coupled plasma mass spectrometry using a rat model. The results demonstrated that the average concentrations of (25)Mg, (28)Si, (39)K, (47)Ti, (56)Fe, (59)Co, (77)Se, (88)Sr, (137)Ba, and (208)Pb were generally higher in tibia than those in maxilla. Compared with the sham group, Ovariectomy induced more significant changes of these elements in tibia than maxilla, indicating tibial trabecular bones are more sensitive to changes of circulating estrogen. In addition, the concentrations of (28)Si, (77)Se, (208)Pb, and Ca/P ratios were higher in tibia and maxilla in ovariectomised rats than those in normal bone at all time-points. The present study indicates that ovariectomy could significantly impact the element distribution and concentrations between tibia and maxilla.
authors.affiliation: Institute of Health and Biomedical innovation, Queensland University of Technology, Brisbane 4059, Australia. p.han@uq.edu.au.|Tissue Engineering and Microfluidics Laboratory, Australian Institute for Bioengineering and Nanotechnology, University of Queensland, St. Lucia 4072, Australia. p.han@uq.edu.au.
authors.forename: Pingping
authors.initials: P
authors.lastname: Han
authors.affiliation: Institute of Health and Biomedical innovation, Queensland University of Technology, Brisbane 4059, Australia. shifeier.lu@connect.qut.edu.au.
authors.forename: Shifeier
authors.initials: S
authors.lastname: Lu
authors.affiliation: Institute of Health and Biomedical innovation, Queensland University of Technology, Brisbane 4059, Australia. y26.zhou@qut.edu.au.
authors.forename: Yinghong
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Institute for Future Environments, Queensland University of Technology, Brisbane 4000, Australia. k.harumimoromizato@qut.edu.au.
authors.forename: Karine
authors.initials: K
authors.lastname: Moromizato
authors.affiliation: Institute of Health and Biomedical innovation, Queensland University of Technology, Brisbane 4059, Australia. zhibin.du@qut.edu.au.
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Du
authors.affiliation: Institute of Health and Biomedical innovation, Queensland University of Technology, Brisbane 4059, Australia. t.friis@qut.edu.au.
authors.forename: Thor
authors.initials: T
authors.lastname: Friis
authors.affiliation: Institute of Health and Biomedical innovation, Queensland University of Technology, Brisbane 4059, Australia. yin.xiao@qut.edu.au.
authors.forename: Yin
authors.initials: Y
authors.lastname: Xiao
mesh_terms: D000818:Animals; D000071556:Cancellous Bone / Q000378:metabolism*; D004967:Estrogens / Q000378:metabolism; D005260:Female; D008437:Maxilla / Q000378:metabolism*; D008670:Metals / Q000378:metabolism*; D010024:Osteoporosis / Q000209:etiology / Q000378:metabolism*; D010052:Ovariectomy / Q000009:adverse effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013977:Tibia / Q000378:metabolism*
chemical_list: D004967:Estrogens; D008670:Metals
keywords: LA-ICP-MS; maxilla; osteoporosis; ovariectomised rats; tibia