title: MiR-19a promotes cell proliferation and invasion by targeting RhoB in human glioma cells.
abstract: MicroRNA-19a (miR-19a) is upregulated in different types of cancers, including gliomas, but its specific role and function in gliomas have yet to be fully elucidated. In this study, we found that miR-19a was significantly upregulated in human glioma tissues and cell lines. Overexpression of miR-19a by a miR-19a mimic promoted glioma cell proliferation and invasion. In contrast, miR-19a inhibitor suppressed cell proliferation and invasion. Furthermore, by a dual-luciferase reporter assay and expression analysis, we determined that Ras homolog family member B was a direct target of miR-19a. Knockdown of Ras homolog family member B could block cell proliferation and invasion induced by the miR-19a mimic. In conclusion, our study demonstrated that miR-19a upregulation is common in gliomas and that suppression of miR-19a expression inhibits cell proliferation and invasion, which indicates that miR-19a may act as an oncogene in gliomas.
authors.affiliation: Department of Neurosurgery, The Third People's Hospital of Shenzhen, Shenzhen, 518112, China.
authors.forename: Qifu
authors.initials: Q
authors.lastname: Chen
authors.affiliation: JiNan University, Second Clinical Medicine College, Shenzhen People's Hospital, Department of Neurosurgery, Shenzhen, 518020, China. Electronic address: weiguoshenz@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Guo
authors.affiliation: JiNan University, Second Clinical Medicine College, Shenzhen People's Hospital, Department of Neurology, Shenzhen, 518020, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: JiNan University, Second Clinical Medicine College, Shenzhen People's Hospital, Department of Neurosurgery, Shenzhen, 518020, China.
authors.forename: Yaochen
authors.initials: Y
authors.lastname: Wu
authors.affiliation: JiNan University, Second Clinical Medicine College, Shenzhen People's Hospital, Department of Neurosurgery, Shenzhen, 518020, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Xiang
mesh_terms: D001932:Brain Neoplasms / Q000378:metabolism / Q000503:physiopathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D055785:Gene Knockdown Techniques; D005910:Glioma / Q000378:metabolism / Q000503:physiopathology*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D009361:Neoplasm Invasiveness / Q000503:physiopathology*; D020743:rhoB GTP-Binding Protein / Q000378:metabolism*
chemical_list: C549214:MIRN19A microRNA, human; D035683:MicroRNAs; D020743:rhoB GTP-Binding Protein
keywords: Glioma; Invasion; MiR-19a; Proliferation; Ras homolog family member B (RhoB)