title: NR2F2 regulates chondrogenesis of human mesenchymal stem cells in bioprinted cartilage.
abstract: Bioprinting as an advanced enabling technology has the capacity to construct tissues with respective anatomical structures. In order to maintain the precise printing resolution for anatomical tissue printing, cell seeding density in bioink is limited. Bone marrow derived mesenchymal stem cells (MSCs) are widely used for cartilage tissue engineering. However, the approach of ideal chondrogenic differentiation of MSCs without hypertrophy still remains elusive. Here, we reported NR2F2 plays a crucial role in MSC chondrogenesis in bioprinted cartilage. NR2F2 over-expressed MSCs showed significantly enhanced chondrogenesis and NR2F2 knockdown cells demonstrated the exactly opposite behavior. We evaluated the cells cultured in monolayer, 3D pellet, and bioprinted 3D scaffold. All observations were consistent among gene expression, biochemical analysis, histological assay, and biomechanical evaluation. The data also revealed possible involvement of NR2F2 in mechanism of MSC chondrogenic differentiation under hypoxic culture condition. Biotechnol. Bioeng. 2017;114: 208-216. © 2016 Wiley Periodicals, Inc.
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, 122 Luoshi Road, Wuhan, China.|Stemorgan Therapeutics, New York, New York.
authors.forename: Guifang
authors.initials: G
authors.lastname: Gao
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, 122 Luoshi Road, Wuhan, China.
authors.forename: Xiao-Fei
authors.initials: XF
authors.lastname: Zhang
authors.affiliation: Stemorgan Therapeutics, New York, New York.
authors.forename: Karen
authors.initials: K
authors.lastname: Hubbell
authors.affiliation: School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, 122 Luoshi Road, Wuhan, China.|Stemorgan Therapeutics, New York, New York.|Department of Biomedical Engineering, Rensselaer Polytechnic Institute, New York, New York.|Clinic for Plastic Surgery and Hand Surgery, Klinikum Rechts der Isar, Technical University Munich, Munich, Germany.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Cui
mesh_terms: D000818:Animals; D062028:Bioprinting / Q000379:methods*; D051837:COUP Transcription Factor II / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D002356:Cartilage; D002454:Cell Differentiation / Q000502:physiology; D015687:Cell Hypoxia; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism; D020219:Chondrogenesis / Q000502:physiology*; D055785:Gene Knockdown Techniques; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D051837:COUP Transcription Factor II; C095569:NR2F2 protein, human
keywords: NR2F2; bioprinting; chondrogenesis; hypoxia; mesenchymal stem cells