title: VRC01 antibody protects against vaginal and rectal transmission of human immunodeficiency virus 1 in hu-BLT mice.
abstract: Broadly neutralizing antibodies (bNAbs) represent a new generation of antiviral agents for the prevention and treatment of human immunodeficiency virus 1 (HIV-1) infection. A better understanding of the in vivo efficacy of HIV-1 bNAbs, such as VRC01, in preventing mucosal transmission of HIV-1 has important implications for HIV-1 vaccine design. In this study, we evaluated the efficacy of passively transferred VRC01 antibody in preventing HIV-1 vaginal and rectal transmission in humanized bone marrow/liver/thymus mice (hu-BLT mice). Mice were subcutaneously injected with VRC01 IgG, and 24 hours later, they were challenged intravaginally or intrarectally with HIV-1Ada. All hu-BLT mice receiving VRC01 IgG antibody were aviremic at 2 weeks after intravaginal (n = 3) or intrarectal (n = 6) challenge as measured by quantitative real-time RT-PCR. In contrast, mice receiving control IgG all became infected. By 5 and 6 weeks post-challenge, some of VRC01 aviremic mice in both the intravaginal and intrarectal challenge groups became viremic. Our results suggest that VRC01 antibody can be protective against HIV-1 vaginal and rectal transmission; however, a single administration of VRC01 cannot completely prevent mucosal infection.
authors.affiliation: Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA.|School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA.|Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Kunming, 650118, Yunnan, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Sun
authors.affiliation: Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA.|School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA.|Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, 300071, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Li
authors.affiliation: Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA.|School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Yuan
authors.affiliation: Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA.|School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA.
authors.forename: Wuxun
authors.initials: W
authors.lastname: Lu
authors.affiliation: Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA.|School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA.
authors.forename: Guobin
authors.initials: G
authors.lastname: Kang
authors.affiliation: Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA.|School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA.
authors.forename: Wenjin
authors.initials: W
authors.lastname: Fan
authors.affiliation: Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA. qli@unl.edu.|School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA. qli@unl.edu.
authors.forename: Qingsheng
authors.initials: Q
authors.lastname: Li
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D057134:Antibodies, Neutralizing; D000080908:Broadly Neutralizing Antibodies; D005260:Female; D015483:HIV Antibodies; D015658:HIV Infections / Q000276:immunology* / Q000517:prevention & control; D015497:HIV-1 / Q000187:drug effects*; D006801:Humans; D007074:Immunoglobulin G; D051379:Mice; D008815:Mice, Inbred Strains; D012007:Rectum / Q000821:virology; D014621:Vagina / Q000821:virology
chemical_list: D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D000080908:Broadly Neutralizing Antibodies; D015483:HIV Antibodies; D007074:Immunoglobulin G; C578595:VRC01 monoclonal antibody