title: Improving the degradation behavior and in vitro biological property of nano-hydroxyapatite surface- grafted with the assist of citric acid.
abstract: To obtain ideal nano-hydroxyapatite(n-HA) filler for poly(lactide-co-glycolide) (PLGA), a new surface-grafting with the assist of citric acid for nano-hydroxyapatite (n-HA) was designed, and the effect of n-HA surface-grafted with or without citric acid on in vitro degradation behavior and cells viability was studied by the experiments of soaking in simulated body fluid (SBF) and incubating with human osteoblast-like cells (MG-63). The change of pH value, tensile strength reduction, the surface deposits, cells attachment and proliferation of samples during the soaking and incubation were investigated by means of pH meter, electromechanical universal tester, scanning electron microscope (SEM) coupled with energy-dispersive spectro-scopy (EDS), fluorescence microscope and MTT method. The results showed that the introduction of citric acid not only delayed the strength reduction during the degradation by inhibiting the detachment of n-HA from PLGA, but also endowed it better cell attachment and proliferation, suggesting that the n-HA surface-grafted with the assist of citric acid was an important bioactive ceramic fillers for PLGA used as bone materials.
authors.affiliation: Key Laboratory of Sustainable Resources Processing and Advanced Materials, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China; Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041, PR China. Electronic address: jlytxg@163.com.
authors.forename: Liuyun
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041, PR China; Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518067, PR China.
authors.forename: Lixin
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041, PR China.
authors.forename: Chengdong
authors.initials: C
authors.lastname: Xiong
authors.affiliation: Key Laboratory of Sustainable Resources Processing and Advanced Materials, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
authors.forename: Shengpei
authors.initials: S
authors.lastname: Su
mesh_terms: D001672:Biocompatible Materials / Q000008:administration & dosage / Q000737:chemistry; D001859:Bone Neoplasms / Q000188:drug therapy / Q000473:pathology; D002448:Cell Adhesion; D049109:Cell Proliferation; D019343:Citric Acid / Q000737:chemistry*; D017886:Durapatite / Q000008:administration & dosage* / Q000737:chemistry; D006801:Humans; D019344:Lactic Acid / Q000008:administration & dosage* / Q000737:chemistry; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D012516:Osteosarcoma / Q000188:drug therapy / Q000473:pathology*; D011100:Polyglycolic Acid / Q000008:administration & dosage* / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D014407:Tumor Cells, Cultured
chemical_list: D001672:Biocompatible Materials; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019343:Citric Acid; D019344:Lactic Acid; D017886:Durapatite
keywords: Biocompatibility; Biodegradation; Hydroxyapatite; Polymer; Surface-modification