title: Unraveling the rat blood genome-wide transcriptome after oral administration of lavender oil by a two-color dye-swap DNA microarray approach.
abstract: Lavender oil (LO) is a commonly used essential oil in aromatherapy as non-traditional medicine. With an aim to demonstrate LO effects on the body, we have recently established an animal model investigating the influence of orally administered LO in rat tissues, genome-wide. In this brief, we investigate the effect of LO ingestion in the blood of rat. Rats were administered LO at usual therapeutic dose (5 mg/kg) in humans, and following collection of the venous blood from the heart and extraction of total RNA, the differentially expressed genes were screened using a 4 × 44-K whole-genome rat chip (Agilent microarray platform; Agilent Technologies, Palo Alto, CA, USA) in conjunction with a two-color dye-swap approach. A total of 834 differentially expressed genes in the blood were identified: 362 up-regulated and 472 down-regulated. These genes were functionally categorized using bioinformatics tools. The gene expression inventory of rat blood transcriptome under LO, a first report, has been deposited into the Gene Expression Omnibus (GEO): GSE67499. The data will be a valuable resource in examining the effects of natural products, and which could also serve as a human model for further functional analysis and investigation.
authors.affiliation: Department of Oral Biology, Graduate School of Dentistry, Tohoku University, Sendai 980-8575, Japan.
authors.forename: Motohide
authors.initials: M
authors.lastname: Hori
authors.affiliation: Department of Anatomy I, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa, Tokyo 142-8555, Japan; Oriental Aromatherapy College, 5-22-9 Kameari, Katsushika, Tokyo 125-0061, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Kubo
authors.affiliation: Department of Anatomy I, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa, Tokyo 142-8555, Japan; Global Research Center for Innovative Life Science, Peptide Drug Innovation, School of Pharmacy and Pharmaceutical Sciences, Hoshi University, 4-41 Ebara 2-chome, Shinagawa, Tokyo 142-8501, Japan.
authors.forename: Junko
authors.initials: J
authors.lastname: Shibato
authors.affiliation: Department of Anatomy I, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa, Tokyo 142-8555, Japan.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Saito
authors.affiliation: Department of Anatomy I, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa, Tokyo 142-8555, Japan; Department of Physiology, Saitama Medical University, 38 Morohongo Moroyama-machi, Iruma-gun, Saitama 350-0495, Japan.
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Ogawa
authors.affiliation: Department of Oral Biology, Graduate School of Dentistry, Tohoku University, Sendai 980-8575, Japan.
authors.forename: Minoru
authors.initials: M
authors.lastname: Wakamori
authors.affiliation: Laboratory of Neuroscience, Department of Biology, Faculty of Science, Toho University, Funabashi, Chiba 274-8510, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Masuo
authors.affiliation: Department of Anatomy I, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa, Tokyo 142-8555, Japan; Global Research Center for Innovative Life Science, Peptide Drug Innovation, School of Pharmacy and Pharmaceutical Sciences, Hoshi University, 4-41 Ebara 2-chome, Shinagawa, Tokyo 142-8501, Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Shioda
authors.affiliation: Department of Anatomy I, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa, Tokyo 142-8555, Japan; Global Research Center for Innovative Life Science, Peptide Drug Innovation, School of Pharmacy and Pharmaceutical Sciences, Hoshi University, 4-41 Ebara 2-chome, Shinagawa, Tokyo 142-8501, Japan; Faculty of Health and Sport Sciences and Tsukuba International Academy for Sport Studies (TIAS), University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8574, Ibaraki, Japan.
authors.forename: Randeep
authors.initials: R
authors.lastname: Rakwal
keywords: Gene expression; Lavender oil ingestion; Rat; Total RNA extraction; Whole blood