title: Paecilomyces variotii: A Fungus Capable of Removing Ammonia Nitrogen and Inhibiting Ammonia Emission from Manure.
abstract: Ammonia (NH3) emissions from animal manure are a significant environmental and public concern. Despite the numerous studies regarding NH3 emissions from manure, few of them have considered microbial nitrification approaches, especially fungal nitrification. In this study, a filamentous fungus was isolated from chicken manure and was used for nitrification. The species was Paecilomyces variotii by morphological characteristics and 18S rDNA gene sequencing. It played the biggest role in the removal of ammonium at pH 4.0-7.0, C/N ratio of 10-40, temperature of 25-37°C, shaking speed of 150 rpm, and with glucose as the available carbon source. Further analysis revealed that all ammonium was removed when the initial ammonium concentration was less than 100 mg/L; 40% ammonium was removed when the initial ammonium concentration was 1100 mg/L. The results showed that the concentration of ammonia from chicken manure with strain Paecilomyces variotii was significantly lower than that in the control group. We concluded that Paecilomyces variotii has good potential for future applications in in situ ammonium removal as well as ammonia emissions control from poultry manure.
authors.affiliation: The key laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Zhiyun
authors.initials: Z
authors.lastname: Liu
authors.affiliation: The key laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Guohua
authors.initials: G
authors.lastname: Liu
authors.affiliation: The key laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Huiyi
authors.initials: H
authors.lastname: Cai
authors.affiliation: The key laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Pengjun
authors.initials: P
authors.lastname: Shi
authors.affiliation: The key laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Wenhuan
authors.initials: W
authors.lastname: Chang
authors.affiliation: The key laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Zhang
authors.affiliation: The key laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Aijuan
authors.initials: A
authors.lastname: Zheng
authors.affiliation: College of Animal Science and Technology, Northeast Agricultural University, Harbin, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Xie
authors.affiliation: The key laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Jianshuang
authors.initials: J
authors.lastname: Ma
mesh_terms: D000641:Ammonia / Q000378:metabolism*; D001673:Biodegradation, Environmental; D008372:Manure / Q000382:microbiology*; D058458:Nitrogen Cycle; D010143:Paecilomyces / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012337:RNA, Ribosomal, 18S / Q000235:genetics
chemical_list: D008372:Manure; D012337:RNA, Ribosomal, 18S; D000641:Ammonia