title: [Down-regulation of TIPE2 promotes the proliferation and immune activity of T lymphocytes].
abstract: Objective To utilize specific small interfering RNA (siRNA) to silence the expression of tumor necrosis factor α-induced protein 8 like-2 (TIPE2) gene of T lymphocytes and investigate the effect of TIPE2 targeting siRNA on T lymphocyte proliferation and immune function. Methods Mouse spleen T lymphocytes were sorted by magnetic beads. Western blotting was used to screen and validate an effective siRNA to silence the TIPE2 gene expression of T lymphocytes. Twenty-four hours after transfection with the siRNA into T lymphocytes, the expression of CD69 in each group was detected by flow cytometry. Seventy-two hours after transfection, the proliferation of the T lymphocytes was measured with CCK-8 assay; meanwhile, the secretion levels of interleukin 2 (IL-2) and interferon γ (IFN-γ) in each group were measured by ELISA. Results We obtained TIPE2 targeting siRNA sequences and effectively silenced the expression of TIPE2 gene. After TIPE2 gene expression was down-regulated, the expression of the CD69 on T lymphocytes increased, and the proliferation of T lymphocytes and the secretion of IL-2 and IFN-γ were enhanced. Conclusion Down-regulation of TIPE2 gene expression can promote the T lymphocyte proliferation and immune activity.
authors.affiliation: Department of Doppler Ultrasound, Yongchuan Hospital, Chongqing Medical University, Chongqing 402160, China.
authors.forename: Lihong
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Thoracic Surgery, Yongchuan Hospital, Chongqing Medical University, Chongqing 402160, China.
authors.forename: Jiangyong
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Thoracic Surgery, Yongchuan Hospital, Chongqing Medical University, Chongqing 402160, China. *Corresponding author, E-mail: hongbin123_fly@163.com.
authors.forename: Bin
authors.initials: B
authors.lastname: Hong
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism; D015153:Blotting, Western; D049109:Cell Proliferation; D002470:Cell Survival / Q000235:genetics; D002478:Cells, Cultured; D015536:Down-Regulation; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D007371:Interferon-gamma / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D037181:Lectins, C-Type / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence; D034622:RNA Interference; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D013997:Time Factors; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D007371:Interferon-gamma; D007376:Interleukin-2; D047908:Intracellular Signaling Peptides and Proteins; D037181:Lectins, C-Type; D000099192:CD69 Antigens; C529002:TIPE2 protein, mouse