title: Automatic quantification of morphological features for hepatic trabeculae analysis in stained liver specimens.
abstract: This paper proposes a digital image analysis method to support quantitative pathology by automatically segmenting the hepatocyte structure and quantifying its morphological features. To structurally analyze histopathological hepatic images, we isolate the trabeculae by extracting the sinusoids, fat droplets, and stromata. We then measure the morphological features of the extracted trabeculae, divide the image into cords, and calculate the feature values of the local cords. We propose a method of calculating the nuclear-cytoplasmic ratio, nuclear density, and number of layers using the local cords. Furthermore, we evaluate the effectiveness of the proposed method using surgical specimens. The proposed method was found to be an effective method for the quantification of the Edmondson grade.
authors.affiliation: Tokyo Institute of Technology, Interdisciplinary Graduate School of Science and Engineering, 4259, Nagatsuta, Midori-ku 226-8503, Japan; Saitama Medical University, Faculty of Health and Medical Care, 1397-1 Yamane, Hidaka-shi Saitama 350-1241, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ishikawa
authors.affiliation: Tokyo Institute of Technology , Interdisciplinary Graduate School of Science and Engineering, 4259, Nagatsuta, Midori-ku 226-8503, Japan.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Murakami
authors.affiliation: Tokyo Institute of Technology , Interdisciplinary Graduate School of Science and Engineering, 4259, Nagatsuta, Midori-ku 226-8503, Japan.
authors.forename: Sercan Taha
authors.initials: ST
authors.lastname: Ahi
authors.affiliation: Tokyo Institute of Technology , Interdisciplinary Graduate School of Science and Engineering, 4259, Nagatsuta, Midori-ku 226-8503, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yamaguchi
authors.affiliation: Saitama Medical University , Faculty of Health and Medical Care, 1397-1 Yamane, Hidaka-shi Saitama 350-1241, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Kobayashi
authors.affiliation: NEC Corporation , Medical Solutions Division, 5-7-1 Shiba Minato-ku, Tokyo 108-8001, Japan.
authors.forename: Tomoharu
authors.initials: T
authors.lastname: Kiyuna
authors.affiliation: NEC Corporation , Medical Solutions Division, 5-7-1 Shiba Minato-ku, Tokyo 108-8001, Japan.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Yamashita
authors.affiliation: Tokyo Medical University, Quantitative Pathology and Immunology Division, 6-1-1- Shinjuku, Sinjyuku-ku Tokyo 160-8402, Japan; Tokyo Medical University, Molecular Pathology Division, 6-1-1- Shinjuku, Sinjyuku-ku, Tokyo 160-8402, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Saito
authors.affiliation: Keio University School of Medicine , Department of Pathology, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
authors.forename: Tokiya
authors.initials: T
authors.lastname: Abe
authors.affiliation: Keio University School of Medicine , Department of Pathology, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
authors.forename: Akinori
authors.initials: A
authors.lastname: Hashiguchi
authors.affiliation: Keio University School of Medicine , Department of Pathology, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
authors.forename: Michiie
authors.initials: M
authors.lastname: Sakamoto
keywords: hematoxylin and eosin-stained specimens; hepatic trabecula; quantitative pathology; segmentation