title: Differential Regulation of Human and Mouse Myometrial Contractile Activity by FSH as a Function of FSH Receptor Density.
abstract: Previous studies from our laboratory revealed that the follicle-stimulating hormone receptor (FSHR) is expressed at low levels in nonpregnant human myometrium and that it is up-regulated in pregnant term nonlaboring myometrium; however, the physiological relevance of these findings was unknown. Herein, we examined signaling pathways stimulated by FSH in immortalized uterine myocytes expressing recombinant FSHR at different densities and showed that cAMP accumulation is stimulated in all cases but that inositol phosphate accumulation is stimulated only at high FSHR densities. Because an increase in cAMP quiets myometrial contractile activity but an increase in 1,4,5-triphosphoinositol stimulates contractile activity, we hypothesized that FSHR density dictates whether FSH quiets or stimulates myometrial contractility. Indeed, in human and mouse nonpregnant myometrium, which express low levels of FSHR, application of FSH resulted in a quieting of contractile activity. In contrast, in pregnant term nonlaboring myometrium, which expresses higher levels of FSHR, application of FSH resulted in increased contractile activity. Examination of pregnant mouse myometrium from different stages of gestation revealed that FSHR levels remained low throughout most of pregnancy. Accordingly, through mid-gestation, the application of FSH resulted in a quieting of contractile activity. At Pregnancy Day (PD) 16.5, FSHR was up-regulated, although not yet sufficiently to mediate stimulation of contractility in response to FSH. This outcome was not observed until PD 19.5, when FSHR was further up-regulated. Our studies describe a novel FSHR signaling pathway that regulates myometrial contractility, and suggest that myometrial FSHR levels dictate the quieting vs. stimulation of uterine contractility in response to FSH.
authors.affiliation: Department of Molecular Physiology and Biophysics, the University of Iowa Carver College of Medicine, Iowa City, Iowa.
authors.forename: Julie A W
authors.initials: JA
authors.lastname: Stilley
authors.affiliation: Department of Molecular Physiology and Biophysics, the University of Iowa Carver College of Medicine, Iowa City, Iowa.
authors.forename: Rongbin
authors.initials: R
authors.lastname: Guan
authors.affiliation: Department of Obstetrics and Gynecology, the University of Iowa Carver College of Medicine, Iowa City, Iowa.
authors.forename: Donna A
authors.initials: DA
authors.lastname: Santillan
authors.affiliation: Department of Obstetrics and Gynecology, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Bryan F
authors.initials: BF
authors.lastname: Mitchell
authors.affiliation: Departments of Internal Medicine and Pharmacology, the University of Iowa Carver College of Medicine, Iowa City, Iowa.
authors.forename: Kathryn G
authors.initials: KG
authors.lastname: Lamping
authors.affiliation: Department of Molecular Physiology and Biophysics, the University of Iowa Carver College of Medicine, Iowa City, Iowa deborah-segaloff@uiowa.edu.
authors.forename: Deborah L
authors.initials: DL
authors.lastname: Segaloff
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D002460:Cell Line; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D006801:Humans; D051379:Mice; D008875:Middle Aged; D032342:Muscle Cells / Q000187:drug effects / Q000378:metabolism; D009213:Myography; D009215:Myometrium / Q000187:drug effects* / Q000502:physiology; D011247:Pregnancy; D011962:Receptors, FSH / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D014590:Uterine Contraction / Q000187:drug effects* / Q000502:physiology; D055815:Young Adult
chemical_list: D011962:Receptors, FSH; D005640:Follicle Stimulating Hormone
keywords: FSH; FSH receptor; contractility; myometrium; parturition