title: Paeoniflorin suppresses inflammatory response in imiquimod-induced psoriasis-like mice and peripheral blood mononuclear cells (PBMCs) from psoriasis patients.
abstract: Psoriasis is one of the most common immune-mediated chronic inflammatory skin disorders, characterized by hyperproliferation of keratinocytes, dilation and growth of dermal capillary vasculature, and cellular infiltration of T cells, dendritic cells (DCs), and neutrophils. Paeoniflorin (PF), the principal component of total glucosides of paeony (TGP), displays anti-inflammatory and antioxidant properties in several animal models. In this study, we investigated the anti-inflammatory effects and mechanisms of PF in imiquimod (IMQ)-induced psoriasis-like mouse model. The effects of PF on inflammatory cytokine expression in peripheral blood mononuclear cells (PBMCs) from patients with psoriasis vulgaris were also observed. Our results indicated that PF effectively attenuated the clinical and histopathologic changes in IMQ-induced psoriasis-like mouse model. Furthermore, PF reduced the infiltration of T cells, CD11c(+)DCs, and neutrophils in lesional skin. In addition, PF also significantly decreased the mRNA expression of inflammatory cytokines, such as IL-17, INF-γ, IL-6, and TNF-α, in IMQ-induced psoriasis-like mouse model and PBMCs from patients with psoriasis vulgaris. Hence, our data suggest that PF can inhibit leukocyte infiltration and decrease the expression of inflammatory cytokines such as IL-17, INF-γ, IL-6, and TNF-α. PF might be a candidate drug for the treatment of psoriasis.
authors.affiliation: a Department of Dermatovenereology, Chengdu Second People's Hospital, Chengdu, 610017 Sichuan, China.|b Department of Dermatovenereology, Chengdu Qingbaijiang Distinct People's Hospital, Chengdu 610300, Sichuan, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.affiliation: a Department of Dermatovenereology, Chengdu Second People's Hospital, Chengdu, 610017 Sichuan, China.
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: Fu
authors.affiliation: a Department of Dermatovenereology, Chengdu Second People's Hospital, Chengdu, 610017 Sichuan, China.
authors.forename: Li-Wen
authors.initials: LW
authors.lastname: Zhang
authors.affiliation: a Department of Dermatovenereology, Chengdu Second People's Hospital, Chengdu, 610017 Sichuan, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Yin
authors.affiliation: a Department of Dermatovenereology, Chengdu Second People's Hospital, Chengdu, 610017 Sichuan, China.
authors.forename: Pei-Mei
authors.initials: PM
authors.lastname: Zhou
authors.affiliation: a Department of Dermatovenereology, Chengdu Second People's Hospital, Chengdu, 610017 Sichuan, China.
authors.forename: Na
authors.initials: N
authors.lastname: Cao
authors.affiliation: a Department of Dermatovenereology, Chengdu Second People's Hospital, Chengdu, 610017 Sichuan, China.
authors.forename: Yong-Hong
authors.initials: YH
authors.lastname: Lu
mesh_terms: D000634:Aminoquinolines / Q000633:toxicity*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology / Q000627:therapeutic use*; D002478:Cells, Cultured; D005960:Glucosides / Q000494:pharmacology / Q000627:therapeutic use*; D006801:Humans; D000077271:Imiquimod; D018836:Inflammation Mediators / Q000037:antagonists & inhibitors* / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D039821:Monoterpenes / Q000494:pharmacology / Q000627:therapeutic use*; D011565:Psoriasis / Q000097:blood / Q000139:chemically induced / Q000517:prevention & control*; D011897:Random Allocation; D006027:Glycosides
chemical_list: D000634:Aminoquinolines; D000894:Anti-Inflammatory Agents, Non-Steroidal; D005960:Glucosides; D000077271:Imiquimod; D018836:Inflammation Mediators; D039821:Monoterpenes; C015423:peoniflorin; D006027:Glycosides
keywords: PBMC; PBMCs; cytokines inflammatoires; imiquimod; inflammatory cytokines; paeoniflorin; paeoniflorine; psoriasis