title: Inhibition of TYRO3/Akt signaling participates in hypoxic injury in hippocampal neurons.
abstract: In this study, we investigated the role of the TYRO3/Akt signaling pathway in hypoxic injury to hippocampal neurons. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed that hypoxia inhibited the proliferation and viability of hippocampal neurons. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay demonstrated that hypoxia induced neuronal apoptosis in a time-dependent manner, with a greater number of apoptotic cells with longer hypoxic exposure. Immunofluorescence labeling revealed that hypoxia suppressed TYRO3 expression. Western blot assay showed that hypoxia decreased Akt phosphorylation levels in a time-dependent manner. Taken together, these findings suggest that hypoxia inhibits the proliferation of hippocampal neurons and promotes apoptosis, and that the inhibition of the TYRO3/Akt signaling pathway plays an important role in hypoxia-induced neuronal injury.
authors.affiliation: School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian Province, China.
authors.forename: Yan-Zhen
authors.identifier: 0000-0001-6090-7497
authors.initials: YZ
authors.lastname: Zhu
authors.affiliation: School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian Province, China.
authors.forename: Na
authors.initials: N
authors.lastname: Xian
authors.affiliation: School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian Province, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Wu
keywords: Akt; TYRO3; apoptosis; hippocampal neurons; hypoxia; nerve regeneration; neural regeneration; primary culture; proliferation