title: Effect of Different Times of Intraperitoneal Injections of Human Bone Marrow Mesenchymal Stem Cell Conditioned Medium on Gentamicin-Induced Acute Kidney Injury.
abstract: OBJECTIVE
  This study examined the effect of mesenchymal stem cells' conditioned media on the severity of acute kidney injury.
  
  
  METHODS
  Acute kidney injury was induced in male rats with 100 mg/kg of gentamicin for six consecutive days intraperitoneally. After inducing the standard model of acute kidney injury, the conditioned medium of 5 &times; 106 cells was calculated for each kilogram of body weight of the rats. Then, it was injected in three different injection patterns other than the baseline injection of gentamicin. The rats were randomly divided into four groups: control group (n = 18) that did not receive any treatment, gentamicin group (n = 18) that received gentamicin at a dosage of 100 mg/kg for six consecutive days intraperitoneally, sham group (n = 54) that received gentamicin for six consecutive days, and an experimental group (n = 54) that received gentamicin for six consecutive days. Serum biochemical analysis and histological changes were studied and analyzed in all groups.
  
  
  RESULTS
  Although human mesenchymal stem cells' conditioned media did not improve serum and tissue markers in the treatment groups, a relative improvement was observed in some indicators of tissue damage.
  
  
  CONCLUSIONS
  Secretory factors of human mesenchymal stem cells can be partly protective against gentamicin-induced nephrotoxicity.&nbsp.
authors.affiliation: Kharazmi University, Tehran, Iran.
authors.forename: Azam
authors.initials: A
authors.lastname: Abedi
authors.affiliation: Department of Animal Biology, Kharazmi University, Tehran, Iran.
authors.forename: Mahnaz
authors.initials: M
authors.lastname: Azarnia
authors.affiliation: Department of pathology, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mansor
authors.initials: M
authors.lastname: Jamali Zahvarehy
authors.affiliation: Department of Animal Biology, Kharazmi University, Tehran, Iran.
authors.forename: Tahereh
authors.initials: T
authors.lastname: Foroutan
authors.affiliation: Kharazmi University, Tehran, Iran.
authors.forename: Sara
authors.initials: S
authors.lastname: Golestani
mesh_terms: D058186:Acute Kidney Injury / Q000139:chemically induced* / Q000517:prevention & control*; D000818:Animals; D000900:Anti-Bacterial Agents; D001854:Bone Marrow Cells; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D005839:Gentamicins / Q000008:administration & dosage / Q000009:adverse effects*; D007274:Injections, Intraperitoneal; D008297:Male; D059630:Mesenchymal Stem Cells; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D017077:Culture Media, Conditioned; D005839:Gentamicins