title: Single cell oil production by Mortierella isabellina from steam exploded corn stover degraded by three-stage enzymatic hydrolysis in the context of on-site enzyme production.
abstract: Single cell oil (SCO), promising as alternative oil source, was produced from steam exploded corn stover (SECS) by Mortierella isabellina. Different bioprocesses from SECS to SCO were compared and the bioprocess C using the three-stage enzymatic hydrolysis was found to be the most efficient one. The bioprocess C used the lowest enzyme input 20FPIU cellulase/g glucan and the shortest time 222h, but produced 44.94g dry cell biomass and 25.77g lipid from 327.63g dry SECS. It had the highest lipid content 57.34%, and its productivities and yields were much higher than those of the bioprocess B and comparable to the bioprocess A, indicating that the three-stage enzymatic hydrolysis could greatly improve the efficiency of the bioprocess from high solid loading SECS to SCO by Mortierella isabellina. This work testified the application value of three-stage enzymatic hydrolysis in lignocellulose-based bioprocesses.
authors.affiliation: College of Life Sciences, Northwest A&F University, 22 Xinong Road, Yangling, Shaanxi 712100, China; National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu 214122, China. Electronic address: fanghao@nwsuaf.edu.cn.
authors.forename: Hao
authors.initials: H
authors.lastname: Fang
authors.affiliation: College of Life Sciences, Northwest A&F University, 22 Xinong Road, Yangling, Shaanxi 712100, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhao
authors.affiliation: College of Life Sciences, Northwest A&F University, 22 Xinong Road, Yangling, Shaanxi 712100, China.
authors.forename: Shaolin
authors.initials: S
authors.lastname: Chen
mesh_terms: D056804:Biofuels; D018533:Biomass; D002480:Cellulase / Q000378:metabolism*; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry / Q000378:metabolism; D020094:Mortierella / Q000737:chemistry / Q000378:metabolism; D013227:Steam; D003313:Zea mays / Q000737:chemistry / Q000378:metabolism
chemical_list: D056804:Biofuels; D013227:Steam; C036909:lignocellulose; D008031:Lignin; D002480:Cellulase
keywords: Mortierella isabellina; On-site enzyme production; Single cell oil; Steam exploded corn stover; Three-stage enzymatic hydrolysis