title: Virulence factors profiles and ESBL production in Escherichia coli causing bacteremia in Peruvian children.
abstract: The presence of 25 virulence genes (VGs), genetic phylogroups, quinolone-resistance and Extended Spectrum β-lactamase (ESBL)-production was assessed in 65 Escherichia coli isolates from blood cultures in children <5 years in Peru. The most frequent VGs were fimA (89.2%), iutA (83.1%), agn43 (72.3%), iucA (67.7%), and fyuA (49.2%). The isolates belonged to D (47.7%), A (26.1%), B1 (21.5%), and B2 (4.6%) phylogroups. D + B2 isolates presented a high number of fimA, hly, papC, sat, and fyuA genes. Quinolone-susceptible (22 isolates - 33.8%) and ESBL-negative (31 isolates - 47.7%) isolates carried more VGs that their respective counterparts (5.7 vs. 4.7 and 5.3 vs. 4.4 respectively); the frequency of the fyuA, aat, aap, and hly genes significantly differed between quinolone-resistant and quinolone-susceptible isolates. Neonatal sepsis isolates tended to be more quinolone-resistant (P = 0.0697) and ESBL-producers (P = 0.0776). Early-onset neonatal sepsis isolates possessed a high number of VGs (5.2 VGs), especially in neonates of ≤1 day (5.9 VGs).
authors.affiliation: Barcelona Institute for Global Health, Barcelona Ctr. Int. Health Res. (CRESIB), Hospital Clínic - Universitat de Barcelona, Barcelona, Spain.
authors.forename: Noemí
authors.initials: N
authors.lastname: Palma
authors.affiliation: Barcelona Institute for Global Health, Barcelona Ctr. Int. Health Res. (CRESIB), Hospital Clínic - Universitat de Barcelona, Barcelona, Spain.
authors.forename: Cláudia
authors.initials: C
authors.lastname: Gomes
authors.affiliation: Instituto de Medicina Tropical Alexander von Humboldt - Universidad Peruana Cayetano Heredia, Lima, Peru.
authors.forename: Maribel
authors.initials: M
authors.lastname: Riveros
authors.affiliation: Instituto de Medicina Tropical Alexander von Humboldt - Universidad Peruana Cayetano Heredia, Lima, Peru.
authors.forename: Wilfredo
authors.initials: W
authors.lastname: García
authors.affiliation: Barcelona Institute for Global Health, Barcelona Ctr. Int. Health Res. (CRESIB), Hospital Clínic - Universitat de Barcelona, Barcelona, Spain.
authors.forename: Sandra
authors.initials: S
authors.lastname: Martínez-Puchol
authors.affiliation: Barcelona Institute for Global Health, Barcelona Ctr. Int. Health Res. (CRESIB), Hospital Clínic - Universitat de Barcelona, Barcelona, Spain.
authors.forename: Lidia
authors.initials: L
authors.lastname: Ruiz-Roldán
authors.affiliation: Barcelona Institute for Global Health, Barcelona Ctr. Int. Health Res. (CRESIB), Hospital Clínic - Universitat de Barcelona, Barcelona, Spain.
authors.forename: Judit
authors.initials: J
authors.lastname: Mateu
authors.affiliation: Instituto de Medicina Tropical Alexander von Humboldt - Universidad Peruana Cayetano Heredia, Lima, Peru.
authors.forename: Coralith
authors.initials: C
authors.lastname: García
authors.affiliation: Institute of Tropical Medicine, Antwerp, Belgium; Department of Microbiology and Immunology, KU Leuven, Leuven, Belgium.
authors.forename: Jan
authors.initials: J
authors.lastname: Jacobs
authors.affiliation: Instituto de Medicina Tropical Alexander von Humboldt - Universidad Peruana Cayetano Heredia, Lima, Peru; University of Texas, Texas School of Public Health, Houston, TX, USA.
authors.forename: Theresa J
authors.initials: TJ
authors.lastname: Ochoa
authors.affiliation: Barcelona Institute for Global Health, Barcelona Ctr. Int. Health Res. (CRESIB), Hospital Clínic - Universitat de Barcelona, Barcelona, Spain. Electronic address: joruiz.trabajo@gmail.com.
authors.forename: Joaquim
authors.initials: J
authors.lastname: Ruiz
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D016470:Bacteremia / Q000382:microbiology*; D001769:Blood / Q000382:microbiology; D002675:Child, Preschool; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000145:classification / Q000201:enzymology* / Q000302:isolation & purification / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology*; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D010568:Peru; D015363:Quinolones / Q000494:pharmacology; D037521:Virulence Factors / Q000032:analysis* / Q000235:genetics; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D015363:Quinolones; D037521:Virulence Factors; D001618:beta-Lactamases
keywords: Bacteremia; ESBL; Extra-intestinal Escherichia coli; Neonatal sepsis; Quinolone-resistance; Virulence