title: Delivery of siRNA Using Cationic Liposomes Incorporating Stearic Acid-modified Octa-Arginine.
abstract: Cationic liposomes incorporating stearic acid-modified octa-arginine (StA-R8) were evaluated for survivin small interfering RNA (siRNA) delivery. StA-R8 was synthesized and incorporated into liposomes. The composition of liposomes was optimized. Physicochemical properties, cytotoxicity, cellular uptake and gene silencing activity of the liposomes complexed to survivin siRNA were investigated. The results showed that StA-R8-containing liposomes had reduced cytotoxicity and improved delivery efficiency of siRNA into cancer cells compared with StA-R8 by itself.
authors.affiliation: College of Animal Science, Jilin University, Changchun, Jilin, P.R. China Department of Chemistry and Pharmacy, Zhuhai College of Jilin University, Zhuhai, Guangdong, P.R. China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Yang
authors.affiliation: College of Life Sciences, Jilin University, Changchun, Jilin, P.R. China.
authors.forename: Yuhuan
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Life Sciences, Jilin University, Changchun, Jilin, P.R. China.
authors.forename: Yuhang
authors.initials: Y
authors.lastname: Qi
authors.affiliation: College of Life Sciences, Jilin University, Changchun, Jilin, P.R. China.
authors.forename: Yongzhen
authors.initials: Y
authors.lastname: Chen
authors.affiliation: College of Life Sciences, Jilin University, Changchun, Jilin, P.R. China.
authors.forename: Xuewei
authors.initials: X
authors.lastname: Yang
authors.affiliation: College of Life Sciences, Jilin University, Changchun, Jilin, P.R. China.
authors.forename: Yujing
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Animal Science, Jilin University, Changchun, Jilin, P.R. China lee.1339@osu.edu songcailiu@jluhp.edu.cn.
authors.forename: Songcai
authors.initials: S
authors.lastname: Liu
authors.affiliation: College of Life Sciences, Jilin University, Changchun, Jilin, P.R. China Division of Pharmaceutics, College of Pharmacy, The Ohio State University, Columbus, OH, U.S.A. lee.1339@osu.edu songcailiu@jluhp.edu.cn.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Lee
mesh_terms: D045744:Cell Line, Tumor; D005434:Flow Cytometry / Q000379:methods; D057809:HEK293 Cells; D006367:HeLa Cells; D056945:Hep G2 Cells; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics*; D008081:Liposomes / Q000008:administration & dosage / Q000737:chemistry; D009842:Oligopeptides / Q000008:administration & dosage* / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000737:chemistry* / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013229:Stearic Acids / Q000008:administration & dosage* / Q000737:chemistry*; D000077022:Survivin; D014162:Transfection / Q000379:methods
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008081:Liposomes; D009842:Oligopeptides; D034741:RNA, Small Interfering; D013229:Stearic Acids; D000077022:Survivin; C500307:octa-arginine peptide; C031183:stearic acid
keywords: Cell-penetrating peptide; cancer; cationic liposomes; octa-arginine; survivin