title: Prolyl hydroxylase 3 (PHD3) expression augments the development of regulatory T cells.
abstract: Regulatory T cells (Tregs) are required for effective immune homeostasis by suppressing harmful immune responses against self-antigens. Transcription factor Foxp3 is required for the development of these cells. How Foxp3 is stabilised and affects Tregs development is still incompletely understood. Previous studies have suggested that hypoxia inducible factor gene HIF-1α negatively influences the development of Tregs and enhances the development of IL-17 producing Th17 cells. In this study, we reveal that prolyl hydroxylase 3 (PHD3), which is a negative regulator of HIF-1α, is upregulated in Tregs and enhances the development of Tregs. The PHD3 inhibitor dimethyl oxalylglycine (DMOG) or siRNAs-PHD3, which upregulates HIF-1α, down-regulated Foxp3 expression, and enhanced the development of Th17 cells. Our observations disclose a novel role of PHD3 in the development of Tregs.
authors.affiliation: Departments of Cardiology, Vascular Medicine and Physiology, Eberhard Karls University of Tubingen, Gmelinstraβe 5, D-72076 Tubingen, Germany. Electronic address: ysinghbt@gmail.com.
authors.forename: Yogesh
authors.initials: Y
authors.lastname: Singh
authors.affiliation: Departments of Cardiology, Vascular Medicine and Physiology, Eberhard Karls University of Tubingen, Gmelinstraβe 5, D-72076 Tubingen, Germany.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Shi
authors.affiliation: Departments of Cardiology, Vascular Medicine and Physiology, Eberhard Karls University of Tubingen, Gmelinstraβe 5, D-72076 Tubingen, Germany; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu City, Sichuan, 611130, PR China.
authors.forename: Shaqiu
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Departments of Cardiology, Vascular Medicine and Physiology, Eberhard Karls University of Tubingen, Gmelinstraβe 5, D-72076 Tubingen, Germany.
authors.forename: Anja T
authors.initials: AT
authors.lastname: Umbach
authors.affiliation: Departments of Cardiology, Vascular Medicine and Physiology, Eberhard Karls University of Tubingen, Gmelinstraβe 5, D-72076 Tubingen, Germany.
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.affiliation: Departments of Cardiology, Vascular Medicine and Physiology, Eberhard Karls University of Tubingen, Gmelinstraβe 5, D-72076 Tubingen, Germany.
authors.forename: Madhuri S
authors.initials: MS
authors.lastname: Salker
authors.affiliation: Departments of Cardiology, Vascular Medicine and Physiology, Eberhard Karls University of Tubingen, Gmelinstraβe 5, D-72076 Tubingen, Germany. Electronic address: florian.lang@uni-tuebingen.de.
authors.forename: Florian
authors.initials: F
authors.lastname: Lang
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000276:immunology*; D002469:Cell Separation; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D055785:Gene Knockdown Techniques; D015151:Immunoblotting; D051379:Mice; D008810:Mice, Inbred C57BL; D016133:Polymerase Chain Reaction; D011393:Procollagen-Proline Dioxygenase / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
chemical_list: C522722:PHD3 protein, mouse; D011393:Procollagen-Proline Dioxygenase
keywords: DMOG; HIF-1α; PHD3; Th17; Tregs