title: Characterization of OsLEA1a and its inhibitory effect on the resistance of E. coli to diverse abiotic stresses.
abstract: OsLEA1a is a late embryogenesis abundant (LEA) protein gene from Oryza sativa L, which contains an open reading frame of 282-bp that encodes a putative polypeptide of 93 amino acids. OsLEA1a protein contains abundant of Lys, Ala, Glu, Asp, Gly, Arg and Leu, but depleted in Cys, His, Phe, Trp and Tyr residues; and is strongly hydrophilic. OsLEA1a includes six helical domains and a β-sheet domain. Real-time PCR analysis showed that OsLEA1a was expressed in roots, leaves and panicles of rice, with no or only a few transcripts in stem tissues, and remained at a relatively higher level in leaves during the tillering period, the heading period, the filling period and the full ripe period. To make sense of OsLEA1a functions, TrxA-OsLEA1a fusion protein expression vector and OsLEA1a protein expression vector were transformed into Escherichia coli DL21 (DE3), respectively. The accumulation of the TrxA-OsLEA1a fusion protein or OsLEA1a protein interfered with the resistance of E. coli to high salinity, metal ions, hyperosmotic, oxidation, heat and freeze-thaw stresses. The purified TrxA-OsLEA1a fusion protein reduced stabilization of LDH and increased damage of diverse abiotic stresses to LDH. The findings suggested that the OsLEA1a may confor antibacterial activity under abiotic stresses.
authors.affiliation: Key Laboratory for Biorheological Science and Technology of Ministry of Education (Chongqing University), Bioengineering College of Chongqing University, Chongqing 400030, China; School of Life Science and Engineering, Chongqing Three Gorges University, Chongqing 404100, China. Electronic address: tzhu2002@foxmail.com.
authors.forename: Tingzhang
authors.initials: T
authors.lastname: Hu
authors.affiliation: School of Life Science and Engineering, Chongqing Three Gorges University, Chongqing 404100, China.
authors.forename: Nong
authors.initials: N
authors.lastname: Zhou
authors.affiliation: Key Laboratory for Biorheological Science and Technology of Ministry of Education (Chongqing University), Bioengineering College of Chongqing University, Chongqing 400030, China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Fu
authors.affiliation: School of Life Science and Engineering, Chongqing Three Gorges University, Chongqing 404100, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Qin
authors.affiliation: School of Life Science and Engineering, Chongqing Three Gorges University, Chongqing 404100, China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Huang
mesh_terms: D019295:Computational Biology; D019327:Copper Sulfate / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development / Q000502:physiology*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D050296:Microbial Viability / Q000187:drug effects; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D060888:Real-Time Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013012:Sorbitol / Q000494:pharmacology; D013312:Stress, Physiological / Q000187:drug effects*
chemical_list: D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; C066246:late embryogenesis abundant protein, plant; D012965:Sodium Chloride; D013012:Sorbitol; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; D019327:Copper Sulfate
keywords: Abiotic stresses; Antibacterial activity; Late embryogenesis abundant protein