title: Calcined Eggshell Waste for Mitigating Soil Antibiotic-Resistant Bacteria/Antibiotic Resistance Gene Dissemination and Accumulation in Bell Pepper.
abstract: The combined accumulation of antibiotics, heavy metals, antibiotic-resistant bacteria (ARB)/antibiotic resistance genes (ARGs) in vegetables has become a new threat to human health. This is the first study to investigate the feasibility of calcined eggshells modified by aluminum sulfate as novel agricultural wastes to impede mixed contaminants from transferring to bell pepper (Capsicum annuum L.). In this work, calcined eggshell amendment mitigated mixed pollutant accumulation in bell pepper significantly, enhanced the dissipation of soil tetracycline, sulfadiazine, roxithromycin, and chloramphenicol, decreased the water-soluble fractions of antibiotics, and declined the diversity of ARB/ARGs inside the vegetable. Moreover, quantitative polymerase chain reaction analysis detected that ARG levels in the bell pepper fruits significantly decreased to 10(-10) copies/16S copies, indicating limited risk of ARGs transferring along the food chain. Furthermore, the restoration of soil microbial biological function suggests that calcined eggshell is an environmentally friendly amendment to control the dissemination of soil ARB/ARGs in the soil-vegetable system.
authors.affiliation: Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences , Nanjing 210008, China.
authors.forename: Mao
authors.initials: M
authors.lastname: Ye
authors.affiliation: Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University , Nanjing, Jiangsu 210095, People's Republic of China.|Department of Soil and Crop Sciences, Texas A&M University , College Station, Texas 88743, United States.
authors.forename: Mingming
authors.initials: M
authors.lastname: Sun
authors.affiliation: Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences , Nanjing, Jiangsu 210014, People's Republic of China.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Department of Civil Engineering, University of Nebraska-Lincoln , 844 North 16th Street, Lincoln, Nebraska 68588-6105, United States.
authors.forename: Xu
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Soil and Crop Sciences, Texas A&M University , College Station, Texas 88743, United States.
authors.forename: Arthur P
authors.initials: AP
authors.lastname: Schwab
authors.affiliation: Nanjing Institute of Environmental Science, Ministry of Environmental Protection of China , Nanjing, Jiangsu 210042, People's Republic of China.
authors.forename: Jinzhong
authors.initials: J
authors.lastname: Wan
authors.affiliation: Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University , Nanjing, Jiangsu 210095, People's Republic of China.
authors.forename: Manqiang
authors.initials: M
authors.lastname: Liu
authors.affiliation: Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University , Nanjing, Jiangsu 210095, People's Republic of China.
authors.forename: Da
authors.initials: D
authors.lastname: Tian
authors.affiliation: Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University , Nanjing, Jiangsu 210095, People's Republic of China.
authors.forename: Kuan
authors.initials: K
authors.lastname: Liu
authors.affiliation: Soil Ecology Lab, College of Resources and Environmental Sciences, Nanjing Agricultural University , Nanjing, Jiangsu 210095, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.affiliation: Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences , Nanjing 210008, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Jiang
mesh_terms: D000327:Adsorption; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D002212:Capsicum / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D024881:Drug Resistance, Bacterial; D004528:Egg Shell / Q000737:chemistry*; D012987:Soil / Q000737:chemistry; D012989:Soil Pollutants / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D012987:Soil; D012989:Soil Pollutants
keywords: antibiotic resistance genes; antibiotic-resistant bacteria; bell pepper cultivation; calcined eggshells; impedance effect