title: Fads1 and 2 are promoted to meet instant need for long-chain polyunsaturated fatty acids in goose fatty liver.
abstract: Global prevalence of non-alcoholic fatty liver disease (NAFLD) constitutes a threat to human health. Goose is a unique model of NAFLD for discovering therapeutic targets as its liver can develop severe steatosis without overt injury. Fatty acid desaturase (Fads) is a potential therapeutic target as Fads expression and mutations are associated with liver fat. Here, we hypothesized that Fads was promoted to provide a protection for goose fatty liver. To test this, goose Fads1 and Fads2 were sequenced. Fads1/2/6 expression was determined in goose liver and primary hepatocytes by quantitative PCR. Liver fatty acid composition was also analyzed by gas chromatography. Data indicated that hepatic Fads1/2/6 expression was gradually increased with the time of overfeeding. In contrast, trans-C18:1n9 fatty acid (Fads inhibitor) was reduced. However, enhanced Fads capacity for long-chain polyunsaturated fatty acid (LC-PUFA) synthesis was not sufficient to compensate for the depleted LC-PUFAs in goose fatty liver. Moreover, cell studies showed that Fads1/2/6 expression was regulated by fatty liver-associated factors. Together, these findings suggest Fads1/2 as protective components are promoted to meet instant need for LC-PUFAs in goose fatty liver, and we propose this is required for severe hepatic steatosis without liver injury.
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.|Colleage of Veterinary Science, West Kordofan University, El Nuhud 20, Sudan.
authors.forename: Rashid H
authors.initials: RH
authors.lastname: Osman
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.
authors.forename: Long
authors.initials: L
authors.lastname: Liu
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Xia
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Zhao
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.
authors.forename: Xiaoxian
authors.initials: X
authors.lastname: Sun
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.
authors.forename: Yihui
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.
authors.forename: Biao
authors.initials: B
authors.lastname: Yang
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China. yzgong@163.com.
authors.forename: Daoqing
authors.initials: D
authors.lastname: Gong
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China. tygeng@yzu.edu.cn.
authors.forename: Tuoyu
authors.initials: T
authors.lastname: Geng
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000096:biosynthesis*; D000090943:Delta-5 Fatty Acid Desaturase; D044943:Fatty Acid Desaturases / Q000096:biosynthesis*; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis*; D005234:Fatty Liver / Q000201:enzymology* / Q000473:pathology / Q000662:veterinary; D005777:Geese / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D022781:Hepatocytes / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D008099:Liver / Q000201:enzymology* / Q000473:pathology
chemical_list: D030161:Avian Proteins; D000090943:Delta-5 Fatty Acid Desaturase; D005231:Fatty Acids, Unsaturated; D044943:Fatty Acid Desaturases; C000716847:FADS1 protein, human
keywords: Cloning; Fatty acid desaturase; Goose; Long-chain polyunsaturated fatty acid; Non-alcoholic fatty liver disease