title: Positive effects of proline addition on the central metabolism of wild-type and lactic acid-producing Saccharomyces cerevisiae strains.
abstract: In Saccharomyces cerevisiae, proline is a stress protectant interacting with other substrate uptake systems against oxidative stress under low pH conditions. In this study, we performed metabolomics analysis to investigate the response associated with an increase in cell growth rates and maximum densities when cells were treated with proline under normal and acid stress conditions. Metabolome data show that concentrations of components of central metabolism are increased in proline-treated S. cerevisiae. No consumption of proline was observed, suggesting that proline does not act as a nutrient but regulates metabolic state and growth of cells. Treatment of lactic acid-producing yeast with proline during lactic acid bio-production improved growth rate and increased the final concentration of lactic acid.
authors.affiliation: Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka, 565-0871, Japan.
authors.forename: Riyanto Heru
authors.initials: RH
authors.lastname: Nugroho
authors.affiliation: Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka, 565-0871, Japan.
authors.forename: Katsunori
authors.initials: K
authors.lastname: Yoshikawa
authors.affiliation: Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka, 565-0871, Japan.
authors.forename: Fumio
authors.initials: F
authors.lastname: Matsuda
authors.affiliation: Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka, 565-0871, Japan. shimizu@ist.osaka-u.ac.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shimizu
mesh_terms: D019344:Lactic Acid / Q000096:biosynthesis*; D055442:Metabolome / Q000187:drug effects*; D011392:Proline / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000254:growth & development*
chemical_list: D019344:Lactic Acid; D011392:Proline
keywords: Acid stress; Lactic acid; Metabolome analysis; Proline; Saccharomyces cerevisiae