title: Immune response to recombinant Leishmania infantum lipophosphoglycan 3 plus CpG oligodeoxynucleotides in BALB/c mice.
abstract: Development of a protective antileishmanial vaccine is an urgent priority for successful control of different forms of leishmaniasis. The potential of a recombinant lipophosphoglycan 3 (rLPG3) expressed by Leishmania tarentolae was evaluated in combination with CpG oligodeoxynucleotides (CpG-ODN) as a Th1-promoting adjuvant against Leishmania infantum infection in BALB/c mice. First, mice were immunized subcutaneously with rLPG3 either alone or in combination with CpG-ODN. Next, the immunogenic and protective efficacies of this vaccine were analysed in immunized mice. It was observed that coadministration of rLPG3 with CpG-ODN led to enhance in a Th1 response to rLPG3 induced by itself as the IFN-γ production was promoted in association with the predominant presence of IgG2a antibodies in the sera. However, immunization with rLPG3 plus CpG-ODN induced partial protection against infectious challenge in BALB/c mice. Taken together, further studies are required to improve the protective efficacy using either more potent immune enhancers or vaccination strategies.
authors.affiliation: Department of Medical Sciences, Ardabil Branch, Islamic Azad University, Ardabil, Iran.
authors.forename: L
authors.initials: L
authors.lastname: Pirdel
authors.affiliation: Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Zavaran Hosseini
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D005260:Female; D006028:Glycosphingolipids / Q000276:immunology*; D000071497:Immunogenicity, Vaccine; D018314:Leishmania infantum / Q000276:immunology*; D007896:Leishmaniasis / Q000276:immunology / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D009838:Oligodeoxyribonucleotides / Q000276:immunology*; D016052:Protozoan Vaccines / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; C408982:CPG-oligonucleotide; D006028:Glycosphingolipids; D009838:Oligodeoxyribonucleotides; D016052:Protozoan Vaccines; C008290:lipophosphonoglycan
keywords:  Leishmania infantum ; CpG-ODN; LPG3; vaccine