title: Life History, Reproductive Biology, and Larval Development of Ontsira mellipes (Hymenoptera: Braconidae), a Newly Associated Parasitoid of the Invasive Asian Longhorned Beetle (Coleoptera: Cerambycidae).
abstract: The invasive Asian longhorned beetle, Anoplophora glabripennis (Motschulsky), is a destructive xylophagous forest pest species originating from Asia. Several endemic North American hymenopteran (Braconidae) species in the mid-Atlantic region were capable of attacking and reproducing on A. glabripennis larvae in laboratory bioassays. Ontsira mellipes Ashmead (Hymenoptera: Braconidae) has been continually reared on A. glabripennis larvae at USDA-ARS BIIRU since 2010, and has been identified as a potential new-association biocontrol agent. Two experiments were conducted to investigate parasitism, paralysis, reproductive biology, larval development, and longevity of adult O. mellipes In the first experiment, pairs of adult parasitoids were given single A. glabripennis larvae every 2 d (along with honey and water) over their lifetimes, while in the second experiment individual parasitoids were observed daily from egg to adult, and adults were subsequently starved. Adults in the first experiment parasitized ∼21% of beetle larvae presented to them throughout their life, and paralysis of larvae occurred 1-2 d after oviposition. More than half of the individual pairs parasitized A. glabripennis larvae, with each female producing around 26 offspring throughout her life. In the second experiment, median development time of O. mellipes from egg to adult was about 3 wk, with five larval instars. Adult O. mellipes that were provided with host larvae, honey, and water lived 9 d longer than host-deprived and starved adults. These findings indicate that mass-rearing procedures for O. mellipes may be developed using the new association host for development of effective biocontrol programs against A. glabripennis.
authors.affiliation: Department of Entomology and Wildlife Ecology, University of Delaware, Newark, DE 19713 (juliang@udel.edu; jhough@udel.edu) United States Department of Agriculture, Agricultural Research Service, Beneficial Insects Introduction Research Unit, Newark, DE 19713 (jian.duan@ars.usda.gov; ellen.aparicio@ars.usda.gov), and juliang@udel.edu.
authors.forename: Julian R
authors.initials: JR
authors.lastname: Golec
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, Beneficial Insects Introduction Research Unit, Newark, DE 19713 (jian.duan@ars.usda.gov; ellen.aparicio@ars.usda.gov), and.
authors.forename: Jian J
authors.initials: JJ
authors.lastname: Duan
authors.affiliation: United States Department of Agriculture, Agricultural Research Service, Beneficial Insects Introduction Research Unit, Newark, DE 19713 (jian.duan@ars.usda.gov; ellen.aparicio@ars.usda.gov), and.
authors.forename: Ellen
authors.initials: E
authors.lastname: Aparicio
authors.affiliation: Department of Entomology and Wildlife Ecology, University of Delaware, Newark, DE 19713 (juliang@udel.edu; jhough@udel.edu).
authors.forename: Judith
authors.initials: J
authors.lastname: Hough-Goldstein
mesh_terms: D000818:Animals; D001517:Coleoptera / Q000254:growth & development / Q000469:parasitology*; D003691:Delaware; D006790:Host-Parasite Interactions; D058865:Introduced Species; D007814:Larva / Q000254:growth & development / Q000469:parasitology / Q000502:physiology; D014863:Wasps / Q000254:growth & development / Q000502:physiology*
keywords: Anoplophora glabripennis; developmental biology; life history; new-association biocontrol; reproductive biology