title: Autophagy induction by sera from women undergoing an in vitro fertilization cycle varies with subsequent outcome.
abstract: Autophagy maintains intracellular homeostasis during placental development and embryogenesis. We evaluated if differences in the autophagy-inducing capacity of sera from women undergoing an in vitro fertilization (IVF) cycle predicted subsequent pregnancy outcome. In this retrospective study, sera collected from 94 women at the time of intrauterine embryo implantation were incubated with peripheral blood mononuclear cells (PBMCs) from healthy donors. The PBMCs were lysed and autophagy induction measured by determination of the p62 concentration. A reduced capacity for autophagy induction was associated with defective implantation while an elevated level of autophagy was associated with ectopic pregnancy.
authors.affiliation: Department of Obstetrics and Gynecology and Ronald O. Perelman and Claudia Cohen Center for Reproductive Medicine and Infertility, Weill Cornell Medicine, New York, NY, USA; Department of Health Sciences, University of Florence, AOUCareggi, Florence, Italy. Electronic address: gis2010@med.cornell.edu.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Sisti
authors.affiliation: Department of Obstetrics and Gynecology and Ronald O. Perelman and Claudia Cohen Center for Reproductive Medicine and Infertility, Weill Cornell Medicine, New York, NY, USA.
authors.forename: Tomi T
authors.initials: TT
authors.lastname: Kanninen
authors.affiliation: Department of Health Sciences, University of Florence, AOUCareggi, Florence, Italy.
authors.forename: Mariarosaria
authors.initials: M
authors.lastname: Di Tommaso
authors.affiliation: Department of Obstetrics and Gynecology and Ronald O. Perelman and Claudia Cohen Center for Reproductive Medicine and Infertility, Weill Cornell Medicine, New York, NY, USA.
authors.forename: Steven S
authors.initials: SS
authors.lastname: Witkin
authors.affiliation: Department of Obstetrics and Gynecology and Ronald O. Perelman and Claudia Cohen Center for Reproductive Medicine and Infertility, Weill Cornell Medicine, New York, NY, USA.
authors.forename: Steven D
authors.initials: SD
authors.lastname: Spandorfer
mesh_terms: D000022:Abortion, Spontaneous / Q000378:metabolism* / Q000628:therapy; D000328:Adult; D001343:Autophagy; D002478:Cells, Cultured; D010064:Embryo Implantation; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D011247:Pregnancy; D011271:Pregnancy, Ectopic / Q000378:metabolism* / Q000628:therapy; D016601:RNA-Binding Proteins / Q000097:blood*; D012189:Retrospective Studies; D044967:Serum / Q000378:metabolism
chemical_list: C118585:P62 protein, human; D016601:RNA-Binding Proteins
keywords: Autophagy; Ectopic pregnancy; In vitro fertilization; Pregnancy outcome