title: Dual Nuclear/Fluorescence Imaging Potantial of Zinc(II) Phthalocyanine in MIA PaCa-2 Cell Line.
abstract: OBJECTIVE
  Pancreatic cancer is very common and difficult to diagnose in early stage. Imaging systems for diagnosing cancer have many disadvantages. However, combining different imaging modalities offers synergistic advantages. Optical imaging is the most multidirectional and widely used imaging modality in both clinical practice and research.
  
  
  METHODS
  In present study, Zinc(II) phthalocyanine [Zn(II)Pc] was synthesized, labeled with iodine- 131 and in vitro study was carried out. The intracellular uptake studies of radiolabeled Zn(II)Pc were performed in WI-38 [ATCC CCL-75™, tissue: human fibroblast lung] and MIA PaCa-2 [ATCC CRL-1420™, tissue: human epithelial pancreas carcinoma] cell lines.
  
  
  RESULTS
  The intracellular uptake efficiency of radiolabeled Zn(II)Pc in MIA PaCa-2 cells was determined two times higher than WI-38 cells. Also, fluorescence imaging (FI) efficiency of synthesized Zn(II)Pc was investigated in MIA PaCa-2 cells and significant uptake was observed.
  
  
  CONCLUSIONS
  Zn(II)Pc might be used as a new agent for dual fluorescence/nuclear imaging for pancreatic cancer.
authors.affiliation: Department of Nuclear Applications, Institute of Nuclear Science, Ege University, Bornova, 35100, Izmir, Turkey.
authors.forename: Fatma Yurt
authors.initials: FY
authors.lastname: Lambrecht
authors.forename: Mine
authors.initials: M
authors.lastname: Ince
authors.forename: Ozge
authors.initials: O
authors.lastname: Er
authors.forename: Kasim
authors.initials: K
authors.lastname: Ocakoglu
authors.forename: Fatma Aslıhan
authors.initials: FA
authors.lastname: Sarı
authors.forename: Cagla
authors.initials: C
authors.lastname: Kayabasi
authors.forename: Cumhur
authors.initials: C
authors.lastname: Gunduz
mesh_terms: D045744:Cell Line, Tumor; D003952:Diagnostic Imaging / Q000379:methods*; D005453:Fluorescence; D006801:Humans; D066298:In Vitro Techniques; D007211:Indoles / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D007457:Iodine Radioisotopes; D054833:Isoindoles; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D010190:Pancreatic Neoplasms / Q000000981:diagnostic imaging*; D011854:Radiochemistry; D019275:Radiopharmaceuticals / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D017967:Zinc Compounds
chemical_list: D007211:Indoles; D007457:Iodine Radioisotopes; D054833:Isoindoles; D009942:Organometallic Compounds; D019275:Radiopharmaceuticals; D017967:Zinc Compounds; C052159:Zn(II)-phthalocyanine
keywords: 131I; MRI; Pancreatic cancer; Zinc(II) Phthalocyanine.; fluorescence imaging; nuclear imaging