title: Identification of key genes associated with cervical cancer by comprehensive analysis of transcriptome microarray and methylation microarray.
abstract: Cervical cancer is the second most commonly diagnosed type of cancer and the third leading cause of cancer-associated mortality in women. The current study aimed to determine the genes associated with cervical cancer development. Microarray data (GSE55940 and GSE46306) were downloaded from Gene Expression Omnibus. Overlapping genes between the differentially expressed genes (DEGs) in GSE55940 (identified by Limma package) and the differentially methylated genes were screened. Gene Ontology (GO) enrichment analysis was subsequently performed for these genes using the ToppGene database. In GSE55940, 91 downregulated and 151 upregulated DEGs were identified. In GSE46306, 561 overlapping differentially methylated genes were obtained through the differential methylation analysis at the CpG site level, CpG island level and gene level. A total of 5 overlapping genes [dipeptidyl peptidase 4 (DPP4); endothelin 3 (EDN3); fibroblast growth factor 14 (FGF14); tachykinin, precursor 1 (TAC1); and wingless-type MMTV integration site family, member 16 (WNT16)] between the 561 overlapping differentially methylated genes and the 242 DEGs were identified, which were downregulated and hypermethylated simultaneously in cervical cancer samples. Enriched GO terms were receptor binding (involvingDPP4, EDN3, FGF14, TAC1andWNT16), ameboidal-type cell migration (DPP4, EDN3andTAC1), mitogen-activated protein kinase cascade (FGF14, EDN3andWNT16) and cell proliferation (EDN3, WNT16, DPP4andTAC1). These results indicate that DPP4, EDN3, FGF14, TAC1 and WNT16 may be involved in the pathogenesis of cervical cancer.
authors.affiliation: Department of Gynecology and Oncology, Tianjin Central Hospital of Gynecology and Obstetrics, Tianjin 300100, P.R. China.
authors.forename: Ming-Yan
authors.initials: MY
authors.lastname: Liu
authors.affiliation: Department of Gynecology and Oncology, Tianjin Central Hospital of Gynecology and Obstetrics, Tianjin 300100, P.R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Gynecology and Oncology, Tianjin Central Hospital of Gynecology and Obstetrics, Tianjin 300100, P.R. China.
authors.forename: Yuan-Jing
authors.initials: YJ
authors.lastname: Hu
authors.affiliation: Department of Obstetrics and Gynecology, Graduate School of Tianjin Medical University, Tianjin 300100, P.R. China.
authors.forename: Yu-Wei
authors.initials: YW
authors.lastname: Chen
authors.affiliation: Department of Obstetrics and Gynecology, Graduate School of Tianjin Medical University, Tianjin 300100, P.R. China.
authors.forename: Xiao-Nan
authors.initials: XN
authors.lastname: Zhao
keywords: cervical cancer; differentially expressed genes; differentially methylated genes; gene ontology enrichment analysis