title: Effect of Otostegia persica extract on ischemia/reperfusion induced renal damage in diabetic rats. A biochemical study.
abstract: OBJECTIVE
  To evaluate the effect of Otostegia persica (O. persica) extract on renal damage induced by ischemia/reperfusion (I/R) in diabetic rats.
  
  
  METHODS
  Forty-eight rats were subjected to right nephrectomy; then, they were allocated into six groups: Sham; Diabetic sham; I/R; Diabetic I/R; I/R+O. persica; Diabetic I/R+O. persica. Diabetes was induced by streptozotocin (200 mg/kg, i.p.). O. persica (300 mg/kg/day, p.o) was administered for 2 weeks. On the 15th day, ischemia was induced in left kidney for 60 min, followed by reperfusion for 24h. Renal functional and biochemical markers were estimated.
  
  
  RESULTS
  I/R in both normal and diabetic rats, induced a significant elevation in serum levels of urea and creatinine (p<0.05). Renal I/R induced a significant increase of malondialdehyde, myeloperoxidase and nitric oxide concentrations associated with significant reduction in superoxide dismutase and catalase activities in comparison with the sham group (p<0.05). Diabetic rats that underwent renal I/R exhibited a significant increase in all the studied parameters with a reduction in the antioxidant enzymes as compared to nondiabetic rats (p<0.05). These deleterious effects associated with renal I/R were improved by the treatment with O. persica (p<0.05).
  
  
  CONCLUSIONS
  Otostegia persica pretreatment protected the renal injury from ischemia-reperfusion in diabetic rats.
authors.affiliation: PhD, Young Researchers and Elites Club, Science and Research Branch, Islamic Azad University, Tehran, Iran. Conception and design of the study; acquisition of data, manuscript writing.
authors.forename: Mohammad Ashrafzadeh
authors.initials: MA
authors.lastname: Takhtfooladi
authors.affiliation: Assistant Professor, Department of Clinical Science, Science and Research Branch, Islamic Azad University, Tehran, Iran. Analysis and interpretation of data, supervised all phases of the study.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Asghari
authors.affiliation: Fellow PhD degree, Department of Clinical Science, Science and Research Branch, Islamic Azad University, Tehran, Iran. Technical procedures, analysis and interpretation of data.
authors.forename: Hesam Aldin
authors.initials: HA
authors.lastname: Hoseinzadeh
authors.affiliation: Graduate student, Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran. Technical procedures, acquisition and interpretation of data.
authors.forename: Farahnaz
authors.initials: F
authors.lastname: Mokhtari
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001786:Blood Glucose / Q000187:drug effects; D002374:Catalase / Q000378:metabolism; D003404:Creatinine / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000150:complications*; D007004:Hypoglycemic Agents / Q000494:pharmacology; D007668:Kidney / Q000187:drug effects; D019686:Lamiaceae; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D023421:Models, Animal; D009392:Nephrectomy / Q000009:adverse effects; D018384:Oxidative Stress / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000150:complications* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014508:Urea / Q000097:blood
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D007004:Hypoglycemic Agents; D008054:Lipid Peroxides; D010936:Plant Extracts; D014508:Urea; D003404:Creatinine; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase