title: Degradation of nicosulfuron by a novel isolated bacterial strain Klebsiella sp. Y1: condition optimization, kinetics and degradation pathway.
abstract: A novel bacterial strain Klebsiella sp. Y1 was isolated from the soil of a constructed wetland, and it was identified based on the 16S rDNA sequence analysis. The co-metabolic degradation of nicosulfuron with glucose by Klebsiella sp. Y1 was investigated. The response surface methodology analysis indicated that the optimal pH and temperature were 7.0 and 35 °C, respectively, for the degradation of nicosulfuron. Under the optimal conditions, the degradation of nicosulfuron fitted Haldane kinetics model well. The removal of nicosulfuron was triggered by the acidification of glucose, which accelerated the hydrolysis of nicosulfuron. Then, the C-N bond of the sulfonylurea bridge was attacked and cleaved. Finally, the detected intermediate 2-amino-4,6-dimethoxypyrimidine was further biodegraded.
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China E-mail: liyongmei@tongji.edu.cn.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China E-mail: liyongmei@tongji.edu.cn.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China E-mail: liyongmei@tongji.edu.cn.
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Li
mesh_terms: D001673:Biodegradation, Environmental; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D007709:Klebsiella / Q000145:classification* / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D011725:Pyridines / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013453:Sulfonylurea Compounds / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D004269:DNA, Bacterial; D011725:Pyridines; D012336:RNA, Ribosomal, 16S; D013453:Sulfonylurea Compounds; D014874:Water Pollutants, Chemical; C416723:nicosulfuron