title: Comparison of the Modified-Hodge test, Carba NP test, and carbapenem inactivation method as screening methods for carbapenemase-producing Enterobacteriaceae.
abstract: We compared three screening methods for carbapenemase-producing Enterobacteriaceae. While the Modified-Hodge test and Carba NP test produced false-negative results for OXA-48-like and mucoid NDM producers, the carbapenem inactivation method (CIM) showed positive results for these isolates. Although the CIM required cultivation time, it is well suited for general clinical laboratories.
authors.affiliation: Department of Clinical Laboratory, Tokyo Metropolitan Health and Medical Treatment Corporation Toshima Hospital, Tokyo, Japan. Electronic address: kageto_yamada@tokyo-hmt.jp.
authors.forename: Kageto
authors.initials: K
authors.lastname: Yamada
authors.affiliation: Department of Clinical Laboratory, Tokyo Metropolitan Health and Medical Treatment Corporation Toshima Hospital, Tokyo, Japan.
authors.forename: Machiko
authors.initials: M
authors.lastname: Kashiwa
authors.affiliation: Department of Clinical Laboratory, Tokyo Metropolitan Health and Medical Treatment Corporation Toshima Hospital, Tokyo, Japan.
authors.forename: Katsumi
authors.initials: K
authors.lastname: Arai
authors.affiliation: Department of Health and Medical Sciences, Shinshu University Graduate School of Medicine, Nagano, Japan; Division of Clinical Microbiology, Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University School of Medicine, Nagano, Japan.
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Nagano
authors.affiliation: Department of Microbiology and Immunology, Graduate School of Health Care Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Ryoichi
authors.initials: R
authors.lastname: Saito
mesh_terms: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins / Q000737:chemistry*; D015780:Carbapenems / Q000737:chemistry; D004755:Enterobacteriaceae / Q000145:classification / Q000201:enzymology / Q000302:isolation & purification*; D000077727:Ertapenem; D015378:Imipenem / Q000737:chemistry; D000077731:Meropenem; D008826:Microbial Sensitivity Tests; D012680:Sensitivity and Specificity; D013845:Thienamycins / Q000737:chemistry; D001618:beta-Lactamases / Q000737:chemistry*; D047090:beta-Lactams / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D013845:Thienamycins; D047090:beta-Lactams; D015378:Imipenem; D001618:beta-Lactamases; C063912:carbapenemase; D000077731:Meropenem; D000077727:Ertapenem
keywords: Carba NP test; Carbapenem inactivation method; Carbapenemase-producing Enterobacteriaceae; Modified-Hodge test