title: Smart platform for the time since death determination from vitreous humor cystine.
abstract: In this work, we report the smart application of AgNPs based sensors for determination of time since death (TSD) via recognition and quantification of vitreous humor (VH) cystine as well as provide the portability for on spot determination of TSD. The lower detection limit was found to be 7.0ng/ml with prominent selectivity. It was found that there is a linear correlation between the VH cystine concentration and TSD as the concentration of cystine increases up to 96h±3.9h. Further for the first time TSD determination is given a smart approach and it proves to have a great utility up to 24h±2.6h. The linear regression equation between TSD (the dependent variable), RGB intensity of cystine concentration till 24h (the independent variable) was found to be TSD=26.69+-0.05*x. The proposed method gives the smart detection, portability, rapidity, sensitivity, selectivity as well as cost effectiveness for determination of time since death.
authors.affiliation: Department of Forensic Science, School of Sciences, Gujarat University, Ahmedabad, Gujarat 380009, India. Electronic address: nihaansari@gujaratuniversity.ac.in.
authors.forename: Niha
authors.initials: N
authors.lastname: Ansari
authors.affiliation: Department of Forensic Science, School of Sciences, Gujarat University, Ahmedabad, Gujarat 380009, India; Institute of Life Sciences, Ahmedabad University, Ahmedabad 380009, Gujarat, India. Electronic address: anand.lodha@ahduni.edu.in.
authors.forename: Anand
authors.initials: A
authors.lastname: Lodha
authors.affiliation: Department of Forensic Science, School of Sciences, Gujarat University, Ahmedabad, Gujarat 380009, India. Electronic address: skmenon@gujaratuniversity.ac.in.
authors.forename: Shobhana K
authors.initials: SK
authors.lastname: Menon
mesh_terms: D001344:Autopsy / Q000295:instrumentation*; D003124:Colorimetry / Q000295:instrumentation*; D003553:Cystine / Q000032:analysis*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D049429:Forensic Pathology / Q000295:instrumentation; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D011180:Postmortem Changes; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012834:Silver / Q000737:chemistry; D000068997:Smartphone; D014822:Vitreous Body / Q000737:chemistry*
chemical_list: D012834:Silver; D003553:Cystine
keywords: Cystine; Nano sensor; Silver nanoparticle; Smart phone; Time since death; Vitreous humor