title: Electrochemical Red Blood Cell Counting: One at a Time.
abstract: We demonstrate that the concentration of a red blood cell solution under physiological conditions can be determined by electrochemical voltammetry. The magnitude of the oxygen reduction currents produced at an edge-plane pyrolytic graphite electrode was diagnosed analytically at concentrations suitable for a point-of-care test device. The currents could be further enhanced when the solution of red blood cells was exposed to hydrogen peroxide. We show that the enhanced signal can be used to detect red blood cells at a single entity level. The method presented relies on the catalytic activity of red blood cells towards hydrogen peroxide and on surface-induced haemolysis. Each single cell activity is expressed as current spikes decaying within a few seconds back to the background current. The frequency of such current spikes is proportional to the concentration of cells in solution.
authors.affiliation: Department of Chemistry, Physical & Theoretical Chemistry Laboratory, University of Oxford, South Parks Road, Oxford, OX1 3QZ, UK.
authors.forename: Lior
authors.initials: L
authors.lastname: Sepunaru
authors.affiliation: Department of Chemistry, Physical & Theoretical Chemistry Laboratory, University of Oxford, South Parks Road, Oxford, OX1 3QZ, UK.
authors.forename: Stanislav V
authors.initials: SV
authors.lastname: Sokolov
authors.affiliation: Department of Materials, University of Oxford, OX1 3PH, UK.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Holter
authors.affiliation: Department of Materials, University of Oxford, OX1 3PH, UK.
authors.forename: Neil P
authors.initials: NP
authors.lastname: Young
authors.affiliation: Department of Chemistry, Physical & Theoretical Chemistry Laboratory, University of Oxford, South Parks Road, Oxford, OX1 3QZ, UK. richard.compton@chem.ox.ac.uk.
authors.forename: Richard G
authors.initials: RG
authors.lastname: Compton
mesh_terms: D002452:Cell Count; D055664:Electrochemical Techniques; D004912:Erythrocytes / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D013997:Time Factors
chemical_list: D006861:Hydrogen Peroxide
keywords: electrochemistry; haemolysis; point-of-care test devices; red blood cells; single cells