title: Heterologous production of α-farnesene in metabolically engineered strains of Yarrowia lipolytica.
abstract: Herein, we studied the heterologous production of α-farnesene, a valuable sesquiterpene with various biotechnological applications, by metabolic engineering of Yarrowia lipolytica. Different overexpression vectors harboring combinations of tHMG1, IDI, ERG20 and codon-optimized α-farnesene synthase (OptFS) genes were constructed and integrated into the genome of Y. lipolytica Po1h. The engineered strain produced 57.08±1.43mg/L of α-farnesene corresponding to 20.8-fold increase over the initial production of 2.75±0.29mg/L in the YPD medium in shake flasks. Bioreactor scale-up in PM medium led to α-farnesene concentration of 259.98±2.15mg/L with α-farnesene to biomass ratio of 33.98±1.51mg/g, which was a 94.5-fold increase over the initial production. This first report on α-farnesene synthesis in Y. lipolytica lays a foundation for future research on production of sesquitepenes in Y. lipolytica and other closest yeast species and will potentially contribute in its industrial production.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Yang
authors.affiliation: Shanghai Collaborative Innovation Center for Biomanufacturing Technology, 130 Meilong Road, Shanghai 200237, China.
authors.forename: Komi
authors.initials: K
authors.lastname: Nambou
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China. Electronic address: weiliujing@ecust.edu.cn.
authors.forename: Liujing
authors.initials: L
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China; Shanghai Collaborative Innovation Center for Biomanufacturing Technology, 130 Meilong Road, Shanghai 200237, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Hua
mesh_terms: D019149:Bioreactors / Q000382:microbiology*; D060847:Metabolic Engineering / Q000379:methods*; D012717:Sesquiterpenes / Q000032:analysis / Q000378:metabolism; D025062:Yarrowia / Q000235:genetics / Q000378:metabolism
chemical_list: D012717:Sesquiterpenes; C062672:alpha-farnesene
keywords: Biofuels; Fed-batch; Metabolic engineering; Terpene synthases; Yarrowia lipolytica