title: Generation of a heterozygous knockout human embryonic stem cell line for the OCIAD1 locus using CRISPR/CAS9 mediated targeting: BJNhem20-OCIAD1-CRISPR-20.
abstract: Ovarian carcinoma immuno-reactive antigen domain containing 1(OCIAD1) single copy was knocked out generating an OCIAD1 heterozygous knockout human embryonic stem line named BJNhem20-OCIAD1-CRISPR-20. The line was generated using CRISPR-Cas9D10A double nickase knockout strategy (Mali et al., 2013).
authors.affiliation: Jawaharlal Nehru Centre for Advanced Scientific Research, Bengaluru, India.
authors.forename: Deeti K
authors.initials: DK
authors.lastname: Shetty
authors.affiliation: Jawaharlal Nehru Centre for Advanced Scientific Research, Bengaluru, India; Institute for Stem Cell Biology and Regenerative Medicine, Bengaluru, India. Electronic address: inamdar@jncasr.ac.in.
authors.forename: Maneesha S
authors.initials: MS
authors.lastname: Inamdar
mesh_terms: D001483:Base Sequence; D015153:Blotting, Western; D064113:CRISPR-Cas Systems / Q000235:genetics*; D002460:Cell Line; D058732:Embryoid Bodies / Q000166:cytology / Q000378:metabolism; D005091:Exons; D055786:Gene Knockout Techniques; D006579:Heterozygote; D000066449:Human Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D059785:Karyotype; D009363:Neoplasm Proteins / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D009363:Neoplasm Proteins; D014157:Transcription Factors; C529235:ovarian cancer immuno-reactive antigen domain containing 1, human