title: Surveying the Delivery Methods of CRISPR/Cas9 for ex vivo Mammalian Cell Engineering.
abstract: The simplicity of the CRISPR/Cas9 technology has been transformative in making targeted genome editing accessible for laboratories around the world. However, due to the sheer volume of literature generated in the past five years, determining the best format and delivery method of CRISPR/Cas9 components can be challenging. Here, we provide a brief overview of the progress that has been made in the ex vivo genome editing of mammalian cells and summarize the key advances made for improving efficiency and delivery of CRISPR/Cas9 in DNA, RNA, and protein form. In particular, we highlight the delivery of Cas9 components to human cells for advanced genome editing applications such as large gene insertion.
authors.affiliation: Department of Biosystems Science and Engineering, ETH Zürich, Basel, Switzerland; National Centre of Competence in Research (NCCR), Molecular Systems Engineering, Switzerland.
authors.forename: William J
authors.initials: WJ
authors.lastname: Kelton
authors.affiliation: Department of Biosystems Science and Engineering, ETH Zürich, Basel, Switzerland; National Centre of Competence in Research (NCCR), Molecular Systems Engineering, Switzerland.
authors.forename: Theresa
authors.initials: T
authors.lastname: Pesch
authors.affiliation: National Centre of Competence in Research (NCCR), Molecular Systems Engineering, Department of Organic Chemistry, University of Geneva, Geneva, Switzerland.
authors.forename: Stefan
authors.initials: S
authors.lastname: Matile
authors.affiliation: Department of Biosystems Science and Engineering ETH Zürich, Basel, Switzerland; National Centre of Competence in Research (NCCR), Molecular Systems Engineering, Switzerland. sai.reddy@ethz.ch.
authors.forename: Sai T
authors.initials: ST
authors.lastname: Reddy
mesh_terms: D000818:Animals; D060846:Cell Engineering; D064112:Clustered Regularly Interspaced Short Palindromic Repeats; D004247:DNA / Q000235:genetics; D066298:In Vitro Techniques; D008322:Mammals; D012333:RNA, Messenger / Q000235:genetics; D012261:Ribonucleoproteins / Q000008:administration & dosage
chemical_list: D012333:RNA, Messenger; D012261:Ribonucleoproteins; D004247:DNA