title: First sporadic case of pathogenic Escherichia coli infection in Black swan in China.
abstract: A strain of bacteria was isolated from the diseased black swan (Cygnus atratus) died from enteritis diarrhea, and designated tentatively as B-1 strain. Morphological and biochemical tests, as well as phylogenetic analysis derived from 16S rRNA and fimC gene sequencing both strongly indicated that B-1 strain is identical to Escherichia coli. Furthermore, the polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) profile of the isolate was different from that of two reference strains. Antibiotic sensitivity testing of B-1 strain was carried out by the standard Kirby-Bauer disc diffusion method. Animal experiments demonstrated that B-1 strain is pathogenic to mice and chickens. This is first sporadic case of pathogenic E. coli infection in Black swan in China.
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, 453003, China.
authors.forename: Lirong
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, 453003, China.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Dong
authors.affiliation: College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, 453003, China.
authors.forename: Sanhu
authors.initials: S
authors.lastname: Wang
authors.affiliation: College of Fisheries, Henan Normal University, Xinxiang, 453007, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: College of Fisheries, Henan Normal University, Xinxiang, 453007, China.
authors.forename: Xianghui
authors.initials: X
authors.lastname: Kong
authors.affiliation: College of Fisheries, Henan Normal University, Xinxiang, 453007, China; Tianjin Key Lab. of Aqua-Ecology & Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin, 300384, China. Electronic address: lajand@126.com.
authors.forename: Aijun
authors.initials: A
authors.lastname: Lv
mesh_terms: D000818:Animals; D046090:Anseriformes; D001431:Bacteriological Techniques; D001715:Bird Diseases / Q000175:diagnosis* / Q000382:microbiology / Q000473:pathology*; D002645:Chickens; D002681:China; D016000:Cluster Analysis; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003967:Diarrhea / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D029968:Escherichia coli Proteins / Q000235:genetics; D036781:Fimbriae Proteins / Q000235:genetics; D051379:Mice; D010802:Phylogeny; D018807:Polymorphism, Single-Stranded Conformational; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004275:DNA, Ribosomal; D029968:Escherichia coli Proteins; D012336:RNA, Ribosomal, 16S; C489721:fimC protein, E coli; D036781:Fimbriae Proteins
keywords: 16S rRNA; Black swan; Escherichia coli; PCR-SSCP; fimC