title: Derivation of the human embryonic stem cell line RCe006-A (RC-2).
abstract: The human embryonic stem cell line RCe006-A (RC-2) was derived from a frozen and thawed blastocyst voluntarily donated as surplus to fertility requirements following ethics committee approved informed consent under licence from the UK Human Fertilisation and Embryology Authority. The cell line exhibits expression of expected pluripotency markers and in vitro differentiation potential to three germinal lineage representative cell populations. It has a male trisomy 12 karyotype (47XY, +12). Microsatellite DNA marker identity and HLA and blood group typing data are available.
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh EH16 4UX, UK; Centre for Clinical Brain Sciences, University of Edinburgh, UK; MRC Centre for Regenerative Medicine, University of Edinburgh, UK. Electronic address: paul.desousa@ed.ac.uk.
authors.forename: P A
authors.initials: PA
authors.lastname: De Sousa
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh EH16 4UX, UK.
authors.forename: B
authors.initials: B
authors.lastname: Tye
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh EH16 4UX, UK.
authors.forename: K
authors.initials: K
authors.lastname: Bruce
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh EH16 4UX, UK.
authors.forename: P
authors.initials: P
authors.lastname: Dand
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh EH16 4UX, UK.
authors.forename: G
authors.initials: G
authors.lastname: Russell
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh EH16 4UX, UK.
authors.forename: J
authors.initials: J
authors.lastname: Gardner
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh EH16 4UX, UK.
authors.forename: J M
authors.initials: JM
authors.lastname: Downie
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh EH16 4UX, UK.
authors.forename: M
authors.initials: M
authors.lastname: Bateman
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh EH16 4UX, UK.
authors.forename: A
authors.initials: A
authors.lastname: Courtney
mesh_terms: D001755:Blastocyst / Q000166:cytology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D002881:Chromosomes, Human, Pair 12; D055028:Comparative Genomic Hybridization; D004314:Down Syndrome / Q000378:metabolism / Q000473:pathology; D058732:Embryoid Bodies / Q000166:cytology; D005838:Genotype; D006650:Histocompatibility Testing; D000066449:Human Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D059785:Karyotype; D008297:Male; D008856:Microscopy, Fluorescence; D016133:Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014314:Trisomy
chemical_list: D014157:Transcription Factors