title: Detection of t(8;14) c-myc/IgH gene rearrangement by long-distance polymerase chain reaction in patients with diffuse large B-cell lymphoma.
abstract: OBJECTIVE
  Specific chromosomal translocations are found in human leukemias and lymphomas. These translocations are closely related to particular histological and immunological phenotypes. In Burkitt's lymphoma, translocation t(8;14)(q24;q32), which involves the c-myc gene (8q24) and the immunoglobulin heavy-chain (IgH) locus (14q32), accounts for 90-95% of all chromosomal translocations. This translocation can be found in 2-5% of diffuse large B-cell lymphoma (DLBCL). Long-distance polymerase chain reaction (LD-PCR) assays, which can identify oncogene/Ig gene rearrangement, can detect these fusion genes. The objective of this study was to detect t(8;14) c-myc/IgH gene rearrangement by LD-PCR in patients with DLBCL.
  
  
  METHODS
  In this study, 54 DLBCL cases were tested by LD-PCR with specific primers. LD-PCR was used for two breakpoints in both the IgH gene (joining region and γ switch region) and the myc gene (Exons 2 and 3).
  
  
  RESULTS
  As much as 1.85% of the samples were positive for the γ constant region and Exon 2 of the myc gene.
  
  
  CONCLUSIONS
  LD-PCR can be used for the detection of t(8;14) c-myc/IgH gene rearrangement in patients with DLBCL.
authors.affiliation: Department of Biology, Tabriz Branch, Islamic Azad University Tabriz, Iran; Department of Biology, College of Science, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
authors.forename: Arezoo
authors.initials: A
authors.lastname: Kiaei
authors.affiliation: Department of Cell and Molecular Biology, Marand Branch, Islamic Azad University, Marand, Iran.
authors.forename: Habib
authors.initials: H
authors.lastname: Onsori
authors.affiliation: Department of Biology, Tabriz Branch, Islamic Azad University Tabriz, Iran; Department of Biology, College of Science, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
authors.forename: Aylar
authors.initials: A
authors.lastname: Alijani
authors.affiliation: Department of Neurosurgery, Poursina Hospital, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
authors.forename: Sasan
authors.initials: S
authors.lastname: Andalib
authors.affiliation: Young Researchers and Elite Club, Ahar Branch, Islamic Azad University, Ahar, Iran.
authors.forename: Saeid
authors.initials: S
authors.lastname: Ghorbian
authors.affiliation: Connective Tissue Research Center, Department of Medical Genetics, Faculty of Medicine and Tabriz Genetic Analysis Centre (TGAC), Tabriz University of Medical Sciences, Daneshgah Street, 516661557, Tabriz, Iran. Electronic address: esakhinia@yahoo.co.uk.
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Sakhinia
mesh_terms: D045744:Cell Line, Tumor; D019457:Chromosome Breakage; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics*; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D004996:Ethidium / Q000378:metabolism; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain / Q000235:genetics*; D006801:Humans; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics*; D014178:Translocation, Genetic / Q000235:genetics*
chemical_list: D016271:Proto-Oncogene Proteins c-myc; D004996:Ethidium
keywords: Diffuse large B-cell lymphoma; Long-distance polymerase chain reaction; Non-Hodgkin lymphoma; Translocation myc/IgH