title: Analysis of microbial community and nitrogen transition with enriched nitrifying soil microbes for organic hydroponics.
abstract: Nitrifying microbial consortia were enriched from bark compost in a water system by regulating the amounts of organic nitrogen compounds and by controlling the aeration conditions with addition of CaCO3for maintaining suitable pH. Repeated enrichment showed reproducible mineralization of organic nitrogen via the conversion of ammonium ions ( ) and nitrite ions ( ) into nitrate ions ( ). The change in microbial composition during the enrichment was investigated by PCR-DGGE analysis with a focus on prokaryote, ammonia-oxidizing bacteria, nitrite-oxidizing bacteria, and eukaryote cell types. The microbial transition had a simple profile and showed clear relation to nitrogen ions transition. Nitrosomonas and Nitrobacter were mainly detected during and oxidation, respectively. These results revealing representative microorganisms acting in each ammonification and nitrification stages will be valuable for the development of artificial simple microbial consortia for organic hydroponics that consisted of identified heterotrophs and autotrophic nitrifying bacteria.
authors.affiliation: a Division of Applied Life Sciences , Graduate School of Agriculture, Kyoto University , Kyoto , Japan.
authors.forename: Sakuntala
authors.initials: S
authors.lastname: Saijai
authors.affiliation: a Division of Applied Life Sciences , Graduate School of Agriculture, Kyoto University , Kyoto , Japan.|b Research Unit for the Physiological Chemistry , Kyoto University , Kyoto , Japan.
authors.forename: Akinori
authors.initials: A
authors.lastname: Ando
authors.affiliation: a Division of Applied Life Sciences , Graduate School of Agriculture, Kyoto University , Kyoto , Japan.
authors.forename: Ryuya
authors.initials: R
authors.lastname: Inukai
authors.affiliation: c National Institute of Vegetable and Tea Science, National Agriculture Research Organization , Tsu , Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Shinohara
authors.affiliation: a Division of Applied Life Sciences , Graduate School of Agriculture, Kyoto University , Kyoto , Japan.|b Research Unit for the Physiological Chemistry , Kyoto University , Kyoto , Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Ogawa
keywords: ammonia-oxidizing bacteria; ammonification; nitrification; nitrite-oxidizing bacteria; organic hydroponics