title: Relative Contribution of Dengue IgG Antibodies Acquired during Gestation or Breastfeeding in Mediating Dengue Disease Enhancement and Protection in Type I Interferon Receptor-Deficient Mice.
abstract: Dengue virus (DENV) causes a spectrum of diseases ranging from self-limiting dengue fever to severe conditions such as haemorrhagic fever and dengue shock syndrome. Antibody-dependent enhancement (ADE) is thought to explain the occurrence of severe dengue whereby pre-existing binding but non-neutralising antibodies enhance DENV infection. The ADE phenomenon is supported by epidemiological findings that infants that born to dengue immune mothers are at greater risk to develop severe dengue upon primary infection. The role of maternally acquired dengue-specific antibodies in disease enhancement was recently recapitulated in a mouse model where mice born to DENV1-immune mothers experienced enhanced disease severity upon DENV2 infection. Here, this study investigates the relative contribution of maternal dengue-specific antibodies acquired during gestation and breastfeeding in dengue disease. Using a surrogate breastfeeding mother experimental approach, we showed that majority of the maternal dengue-specific antibodies were acquired during breastfeeding and conferred an extended enhancement window. On the other hand, in the context of homologous infection, breastfeeding conferred protection. Furthermore, measurement of dengue-specific antibody titres over time in mice born to dengue immune mothers revealed a biphasic pattern of antibody decay as reported in humans. Our work provides evidence of the potential contribution of breast milk-acquired dengue-specific IgG antibodies in enhancement and protection against dengue. Should such contribution be established in humans as well, it may have important implications for the development of guidelines to dengue-immune breastfeeding mothers.
authors.affiliation: Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.|Immunology Programme, Life Sciences Institute, National University of Singapore, Singapore.
authors.forename: Pei Xuan
authors.initials: PX
authors.lastname: Lee
authors.affiliation: Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.|Immunology Programme, Life Sciences Institute, National University of Singapore, Singapore.
authors.forename: Li Ching
authors.initials: LC
authors.lastname: Ong
authors.affiliation: Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.|Immunology Programme, Life Sciences Institute, National University of Singapore, Singapore.
authors.forename: Eshele Anak
authors.initials: EA
authors.lastname: Libau
authors.affiliation: Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.|Immunology Programme, Life Sciences Institute, National University of Singapore, Singapore.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Alonso
mesh_terms: D000375:Aging; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D001942:Breast Feeding; D002460:Cell Line; D006224:Cricetinae; D003715:Dengue / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control; D003716:Dengue Virus / Q000276:immunology*; D005260:Female; D007074:Immunoglobulin G / Q000276:immunology*; D007370:Interferon Type I / Q000235:genetics / Q000378:metabolism*; D007774:Lactation; D051379:Mice; D018345:Mice, Knockout; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control; D010948:Viral Plaque Assay
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007370:Interferon Type I