title: Therapeutic effects of adipose-derived stem cells-based microtissues on erectile dysfunction in streptozotocin-induced diabetic rats.
abstract: This study aimed to explore the therapeutic effects of adipose-derived stem cells (ADSCs)-based microtissues (MTs) on erectile dysfunction (ED) in streptozotocin (STZ)-induced diabetic rats. Fifty-six 8-week-old Sprague-Dawley rats received intraperitoneal injection of STZ (60 mg kg-1 ), and 8 weeks later, the determined diabetic rats randomly received intracavernous (IC) injection of phosphate buffer solution (PBS), ADSCs, or MTs. Another eight normal rats equally got IC injection of PBS. MTs were generated with a hanging drop method, and the injected cells were tracked in ADSC- and MT-injected rats. Four weeks after the treatments, intracavernous pressure (ICP), histopathological changes in corpus cavernosum (CC), and functional proteins were measured. Rat cytokine antibody array was used to detect ADSCs or MTs lysate. The results showed that MTs expressed vascular endothelial growth factor (VEGF), nerve growth factor (NGF), and tumor necrosis factor-stimulated gene-6 (TSG-6). MTs injection had a higher retention than ADSCs injection and MTs treatment improved ICP, neuronal nitric oxide synthase (nNOS) expression, smooth muscle, and endothelial contents in diabetic rats, ameliorated local inflammation in CC better. Thus, our findings demonstrate that IC injection of MTs improves erectile function and histopathological changes in STZ-induced diabetic rats and appears to be more promising than traditional ADSCs. The underlying mechanisms involve increased cell retention accompanied with neuroprotection and anti-inflammatory behaviors of the paracrine factors.
authors.affiliation: Molecular Biology Laboratory of Andrology Center, Peking University First Hospital, Peking University, Beijing 100034, China.|Department of Urology, First Affiliated Hospital of Soochow University, Soochow University, Suzhou 215006, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhou
authors.affiliation: Molecular Biology Laboratory of Andrology Center, Peking University First Hospital, Peking University, Beijing 100034, China.|Department of Urology, First Affiliated Hospital of Soochow University, Soochow University, Suzhou 215006, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Hui
authors.affiliation: Department of Ophthalmology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100043, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Xin
authors.affiliation: Molecular Biology Laboratory of Andrology Center, Peking University First Hospital, Peking University, Beijing 100034, China.
authors.forename: Yong-De
authors.initials: YD
authors.lastname: Xu
authors.affiliation: Molecular Biology Laboratory of Andrology Center, Peking University First Hospital, Peking University, Beijing 100034, China.
authors.forename: Hong-En
authors.initials: HE
authors.lastname: Lei
authors.affiliation: Molecular Biology Laboratory of Andrology Center, Peking University First Hospital, Peking University, Beijing 100034, China.
authors.forename: Bi-Cheng
authors.initials: BC
authors.lastname: Yang
authors.affiliation: Molecular Biology Laboratory of Andrology Center, Peking University First Hospital, Peking University, Beijing 100034, China.
authors.forename: Rui-Li
authors.initials: RL
authors.lastname: Guan
authors.affiliation: Department of Urology, General Hospital of Ningxia Medical University, Ningxia Medical University, Ningxia 750021, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Urology, First Affiliated Hospital of Soochow University, Soochow University, Suzhou 215006, China.
authors.forename: Jian-Quan
authors.initials: JQ
authors.lastname: Hou
authors.affiliation: Molecular Biology Laboratory of Andrology Center, Peking University First Hospital, Peking University, Beijing 100034, China.
authors.forename: Zhong-Cheng
authors.initials: ZC
authors.lastname: Xin
mesh_terms: D000199:Actins / Q000378:metabolism; D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D015153:Blotting, Western; D015815:Cell Adhesion Molecules / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000150:complications* / Q000378:metabolism; D004195:Disease Models, Animal; D007172:Erectile Dysfunction / Q000209:etiology / Q000378:metabolism / Q000628:therapy*; D005455:Fluorescent Antibody Technique; D008297:Male; D016328:NF-kappa B / Q000378:metabolism; D020932:Nerve Growth Factor / Q000378:metabolism; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism; D010410:Penile Erection; D010413:Penis / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D000199:Actins; D015815:Cell Adhesion Molecules; D016328:NF-kappa B; D020932:Nerve Growth Factor; D052248:Nitric Oxide Synthase Type I; D042461:Vascular Endothelial Growth Factor A; C513056:Tnfaip6 protein, rat; C498862:Acta2 protein, rat; C467498:vascular endothelial growth factor A, rat