title: Sesamin protects against renal ischemia reperfusion injury by promoting CD39-adenosine-A2AR signal pathway in mice.
abstract: Ischemia reperfusion injury (IRI) is a leading cause of acute kidney injury with high morbidity and mortality due to limited therapy. Here, we examine whether sesamin attenuates renal IRI in an animal model and explore the underlying mechanisms. Male mice were subjected to right renal ischemia for 30 min followed by reperfusion for 24 h with sesamin (100 mg/kg) during which the left kidney was removed. Renal damage and function were assessed subsequently. The results showed that sesamin reduced kidney ischemia reperfusion injury, as assessed by decreased serum creatinine (Scr) and Blood urea nitrogen (BUN), alleviated tubular damage and apoptosis. In addition, sesamin inhibited neutrophils infiltration and pro-inflammatory cytokines tumor necrosis factor (TNF)-α and interleukin (IL)-1β production in IR-preformed kidney. Notably, sesamin promoted the expression of CD39, A2A adenosine receptor (A2AAR), and A2BAR mRNA and protein as well as adenosine production. Furthermore, CD39 inhibitor or A2AR antagonist abolished partly the protection of sesamin in kidney IRI. In conclusion, sesamin could effectively protect kidney from IRI by inhibiting inﬂammatory responses, which might be associated with promoting the adenosine-CD39-A2AR signaling pathway.
authors.affiliation: Department of Orthopaedic Surgery, The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016, China; Department of Anesthesiology, The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Li
authors.affiliation: Department of Anatomy, Chongqing Medical University Chongqing 400016, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Gong
authors.affiliation: Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology, Chongqing Medical University Chongqing 400016, China.
authors.forename: Ge
authors.initials: G
authors.lastname: Kuang
authors.affiliation: Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University Chongqing 400016, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Jiang
authors.affiliation: Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology, Chongqing Medical University Chongqing 400016, China.
authors.forename: Jingyuan
authors.initials: J
authors.lastname: Wan
authors.affiliation: Department of Orthopaedic Surgery, The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016, China; Department of Anesthesiology, The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
keywords: A2 adenosine receptors (A2AR); CD39; Renal ischemia reperfusion injury; adenosine; sesamin