title: A Natural Cattle Immune Response Against Horn Fly (Diptera: Muscidae) Salivary Antigens May Regulate Parasite Blood Intake.
abstract: The horn fly, Haematobia irritans (L.), is a blood-sucking ectoparasite that is responsible for sizeable economic losses in livestock. The salivary gland products facilitate blood intake. Taking advantage of the identification of novel H. irritans salivary antigens (Hematobin, HTB and Irritans 5, IT5), we investigated the parasite loads, H. irritans blood intake, and antibody response of naturally infected bovines during the fly season. Fly loads and fly hemoglobin content fluctuated during the trial. Each time horn fly loads exceeded 200 flies per cattle, a reduction in horn fly blood intake was observed three weeks later. All of the cattle elicited an antibody response against HTB and IT5 that declined once the fly season was over. Cattle anti-IT5 titers were positively correlated with parasite loads and negatively correlated with fly blood intake. These results suggest that the natural changes in the H. irritans blood intake observed in this study were associated with a natural host response against horn fly salivary antigens.
authors.affiliation: Unidad de Reactivos y Biomodelos de Experimentación, Facultad de Medicina, Universidad de la República, Gral. Flores 2125, Montevideo, Uruguay (mbreijo@fmed.edu.uy; gabrielrocha85@gmail.com; ximenaures@gmail.com; palonzo@higiene.edu.uy; msantos@fmed.edu.uy) mbreijo@fmed.edu.uy.
authors.forename: M
authors.initials: M
authors.lastname: Breijo
authors.affiliation: Laboratorio de Interacciones Moleculares, Facultad de Ciencias, Iguá 4225, 11400 Montevideo, Uruguay (lpastro@fcien.edu.uy).
authors.forename: L
authors.initials: L
authors.lastname: Pastro
authors.affiliation: Unidad de Reactivos y Biomodelos de Experimentación, Facultad de Medicina, Universidad de la República, Gral. Flores 2125, Montevideo, Uruguay (mbreijo@fmed.edu.uy; gabrielrocha85@gmail.com; ximenaures@gmail.com; palonzo@higiene.edu.uy; msantos@fmed.edu.uy).
authors.forename: S
authors.initials: S
authors.lastname: Rocha
authors.affiliation: Unidad de Reactivos y Biomodelos de Experimentación, Facultad de Medicina, Universidad de la República, Gral. Flores 2125, Montevideo, Uruguay (mbreijo@fmed.edu.uy; gabrielrocha85@gmail.com; ximenaures@gmail.com; palonzo@higiene.edu.uy; msantos@fmed.edu.uy).
authors.forename: X
authors.initials: X
authors.lastname: Ures
authors.affiliation: Unidad de Reactivos y Biomodelos de Experimentación, Facultad de Medicina, Universidad de la República, Gral. Flores 2125, Montevideo, Uruguay (mbreijo@fmed.edu.uy; gabrielrocha85@gmail.com; ximenaures@gmail.com; palonzo@higiene.edu.uy; msantos@fmed.edu.uy).
authors.forename: P
authors.initials: P
authors.lastname: Alonzo
authors.affiliation: Unidad de Reactivos y Biomodelos de Experimentación, Facultad de Medicina, Universidad de la República, Gral. Flores 2125, Montevideo, Uruguay (mbreijo@fmed.edu.uy; gabrielrocha85@gmail.com; ximenaures@gmail.com; palonzo@higiene.edu.uy; msantos@fmed.edu.uy).
authors.forename: M
authors.initials: M
authors.lastname: Santos
authors.affiliation: Departamento de Histología y Embriología, Facultad de Medicina, Universidad de la República, Gral. Flores 2125, Montevideo, Uruguay (cbolatto@fmed.edu.uy).
authors.forename: C
authors.initials: C
authors.lastname: Bolatto
authors.affiliation: Cátedra de Inmunología, Facultad de Química, Instituto de Higiene, Universidad de la República, Av. Alfredo Navarro 3051, 11600 Montevideo, Uruguay (cfernan@fq.edu.uy).
authors.forename: C
authors.initials: C
authors.lastname: Fernández
authors.affiliation: Laboratorio de Técnicas Nucleares, Facultad de Veterinaria, Universidad de la República, Lasplaces 1550, Montevideo, Uruguay (meikleana@gmail.com).
authors.forename: A
authors.initials: A
authors.lastname: Meikle
mesh_terms: D000818:Animals; D000917:Antibody Formation; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology*; D004478:Ectoparasitic Infestations / Q000276:immunology / Q000662:veterinary*; D005260:Female; D006790:Host-Parasite Interactions; D008297:Male; D009117:Muscidae / Q000737:chemistry / Q000502:physiology*; D012463:Saliva / Q000737:chemistry
keywords: H. irritans; antibody response; blood feeding; cattle; salivary antigen