title: Vagotomy diminishes obesity in cafeteria rats by decreasing cholinergic potentiation of insulin release.
abstract: Herein, we investigated whether subdiaphragmatic vagotomy has benefits on obesity, body glucose homeostasis, and insulin secretion in cafeteria (CAF)-obese rats. Wistar rats were fed a standard or CAF diet for 12 weeks. Subsequently, CAF rats were randomly submitted to truncal vagotomy (CAF Vag) or sham operation (CAF Sham). CAF Sham rats were hyperphagic, obese, and presented metabolic disturbances, including hyperinsulinemia, glucose intolerance, insulin resistance, hyperglycemia, and hypertriglyceridemia. Twelve weeks after vagotomy, CAF Vag rats presented reductions in body weight and perigonadal fat stores. Vagotomy did not modify glucose tolerance but normalized fed glycemia, insulinemia, and insulin sensitivity. Isolated islets from CAF Sham rats secreted more insulin in response to the cholinergic agent, carbachol, and when intracellular cyclic adenine monophosphate (cAMP) is enhanced by forskolin or 3-isobutyl-1-methylxanthine. Vagotomy decreased glucose-induced insulin release due to a reduction in the cholinergic action on β-cells. This effect also normalized islet secretion in response to cAMP. Therefore, vagotomy in rats fed on a CAF-style diet effectively decreases adiposity and restores insulin sensitivity. These effects were mainly associated with the lack of cholinergic action on the endocrine pancreas, which decreases insulinemia and may gradually reduce fat storage and improve insulin sensitivity.
authors.affiliation: Laboratório de Fisiologia Endócrina e Metabolismo, Centro de Ciências Biológicas e da Saúde, UNIOESTE, Cascavel, PR, 858119-110, Brazil.
authors.forename: Sandra Lucinei
authors.initials: SL
authors.lastname: Balbo
authors.affiliation: UFRJ, Macaé, RJ, Brazil.
authors.forename: Rosane Aparecida
authors.initials: RA
authors.lastname: Ribeiro
authors.affiliation: Laboratório de Fisiologia Endócrina e Metabolismo, Centro de Ciências Biológicas e da Saúde, UNIOESTE, Cascavel, PR, 858119-110, Brazil.
authors.forename: Mariana Carla
authors.initials: MC
authors.lastname: Mendes
authors.affiliation: Laboratório de Fisiologia Endócrina e Metabolismo, Centro de Ciências Biológicas e da Saúde, UNIOESTE, Cascavel, PR, 858119-110, Brazil.
authors.forename: Camila
authors.initials: C
authors.lastname: Lubaczeuski
authors.affiliation: Laboratório de Fisiologia Endócrina e Metabolismo, Centro de Ciências Biológicas e da Saúde, UNIOESTE, Cascavel, PR, 858119-110, Brazil.
authors.forename: Ana Claudia Paiva Alegre
authors.initials: AC
authors.lastname: Maller
authors.affiliation: Laboratório de Pâncreas Endócrino e Metabolismo, Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, UNICAMP, Campinas, SP, Brazil.
authors.forename: Everardo Magalhães
authors.initials: EM
authors.lastname: Carneiro
authors.affiliation: Laboratório de Fisiologia Endócrina e Metabolismo, Centro de Ciências Biológicas e da Saúde, UNIOESTE, Cascavel, PR, 858119-110, Brazil. mlbonfleur@hotmail.com.
authors.forename: Maria Lúcia
authors.initials: ML
authors.lastname: Bonfleur
mesh_terms: D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002217:Carbachol / Q000494:pharmacology; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D059305:Diet, High-Fat; D004195:Disease Models, Animal; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D006943:Hyperglycemia / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D006946:Hyperinsulinism / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D015228:Hypertriglyceridemia / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D007328:Insulin / Q000378:metabolism; D007333:Insulin Resistance; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009765:Obesity / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D051381:Rats; D017208:Rats, Wistar; D046509:Tissue Culture Techniques; D014628:Vagotomy; D014630:Vagus Nerve / Q000378:metabolism / Q000601:surgery*
chemical_list: D007328:Insulin; D005576:Colforsin; D002217:Carbachol; D000242:Cyclic AMP; D005947:Glucose; D015056:1-Methyl-3-isobutylxanthine
keywords: Cafeteria diet; Glucose homeostasis; Insulin secretion; Obesity; Truncal vagotomy