title: [Isolation and biological characteristics on Yersinia pestis phage YP060].
abstract: OBJECTIVE
  To isolate and identify the characteristics of Yersinia pestis phage YP060 from mice nests in Yunnan plague focus.
  
  
  METHODS
  Phage YP060 was isolated from Yunnan plague focus by double-layer agar plate method with attenuated vaccine Yersinia pestis strain EV76 as the host bacterium. Phage particle was observed under the electron microscope while biological properties of phage YP060 including lytic capacity, host spectrum, optimal multiplicity of infection (MOI), one-step growth curve, sensitivity to temperature, pH, ultraviolet ray and chloroform etc. were identified.
  
  
  RESULTS
  The particles of phage YP060 presented tadpoles-like features, with optimal multiplicity of infection as 0.1. Latent period and burst period appeared as 50 min and 80 min, respectively. The phage endured the temperature between 30-50 ℃, and it contained a stronger lysis activities in a range of pH 5 to 10. Phage YP060 was sensitive to ultraviolet rays but insensitive to chloroform while presented special lysic ability to Yersinia pestis.
  
  
  CONCLUSIONS
  Yersinia pestis phage YP060 was isolated for the first time from mice nests in plague natural focus in China. Our findings related to narrow host spectrum and stronger lysis activities.
authors.affiliation: State Key Laboratory for Communicable Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Graduate School, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Department of Plague Control and Prevention, Qinghai Institute for Endemic Disease Control and Prevention, Xining 811602, China.
authors.forename: C X
authors.initials: CX
authors.lastname: Li
authors.affiliation: Provincial Key Laboratory for Zoonosis Control and Prevention, Yunnan Institute for Endemic Disease Control and Prevention, Dali 671000, China.
authors.forename: P
authors.initials: P
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Communicable Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: X N
authors.initials: XN
authors.lastname: Shen
authors.affiliation: Provincial Key Laboratory for Zoonosis Control and Prevention, Yunnan Institute for Endemic Disease Control and Prevention, Dali 671000, China.
authors.forename: H P
authors.initials: HP
authors.lastname: Zhang
authors.affiliation: Provincial Key Laboratory for Zoonosis Control and Prevention, Yunnan Institute for Endemic Disease Control and Prevention, Dali 671000, China.
authors.forename: Y B
authors.initials: YB
authors.lastname: Ding
authors.affiliation: Provincial Key Laboratory for Zoonosis Control and Prevention, Yunnan Institute for Endemic Disease Control and Prevention, Dali 671000, China.
authors.forename: W J
authors.initials: WJ
authors.lastname: Chen
authors.affiliation: Department of Plague Control and Prevention, Yulong Center for Disease Control and Prevention, Lijiang 674100, China.
authors.forename: Y T
authors.initials: YT
authors.lastname: He
authors.affiliation: Department of Plague Control and Prevention, Yulong Center for Disease Control and Prevention, Lijiang 674100, China.
authors.forename: Q G
authors.initials: QG
authors.lastname: He
authors.affiliation: State Key Laboratory for Communicable Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Executive Office, Tibet Autonomous Region Center for Disease Control and Prevention, Lasa 850000, China.
authors.forename: Z J
authors.initials: ZJ
authors.lastname: Li
authors.affiliation: State Key Laboratory for Communicable Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: W
authors.initials: W
authors.lastname: Li
mesh_terms: D000818:Animals; D001435:Bacteriophages; D051379:Mice; D010930:Plague; D015010:Yersinia pestis