title: Derivation of the human embryonic stem cell line RCe012-A (RC-8).
abstract: The human embryonic stem cell line RCe012-A (RC-8) was derived from a frozen and thawed day 5 embryo cultivated to the blastocyst stage. The embryo was voluntarily donated as unsuitable and surplus to fertility requirements following ethics committee approved informed consent under licence from the UK Human Fertilisation and Embryology Authority. The cell line shows normal pluripotency marker expression and differentiation to the three germ layers in vitro. It has a normal 46XX female karyotype and microsatellite PCR identity, HLA and blood group typing data is available.
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh, EH16 4UX, UK; Centre for Clinical Brain Sciences, University of Edinburgh, UK; MRC Centre for Regenerative Medicine, University of Edinburgh, UK.
authors.forename: P A
authors.initials: PA
authors.lastname: De Sousa
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh, EH16 4UX, UK.
authors.forename: B J
authors.initials: BJ
authors.lastname: Tye
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh, EH16 4UX, UK.
authors.forename: K
authors.initials: K
authors.lastname: Bruce
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh, EH16 4UX, UK.
authors.forename: P
authors.initials: P
authors.lastname: Dand
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh, EH16 4UX, UK.
authors.forename: G
authors.initials: G
authors.lastname: Russell
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh, EH16 4UX, UK.
authors.forename: D M
authors.initials: DM
authors.lastname: Collins
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh, EH16 4UX, UK.
authors.forename: A
authors.initials: A
authors.lastname: Greenshields
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh, EH16 4UX, UK.
authors.forename: H
authors.initials: H
authors.lastname: Bradburn
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh, EH16 4UX, UK.
authors.forename: J M
authors.initials: JM
authors.lastname: Downie
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh, EH16 4UX, UK.
authors.forename: M
authors.initials: M
authors.lastname: Bateman
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh, EH16 4UX, UK.
authors.forename: A
authors.initials: A
authors.lastname: Courtney
mesh_terms: D000483:Alleles; D001755:Blastocyst / Q000166:cytology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D058732:Embryoid Bodies / Q000166:cytology; D005260:Female; D005838:Genotype; D006650:Histocompatibility Testing; D000066449:Human Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D059785:Karyotype; D016256:Lewis X Antigen / Q000378:metabolism; D018895:Microsatellite Repeats / Q000235:genetics; D008856:Microscopy, Fluorescence; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D016133:Polymerase Chain Reaction
chemical_list: D016256:Lewis X Antigen; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human