title: Identification of pathway-related modules in high-grade osteosarcoma based on topological centrality of network strategy.
abstract: The objective of this paper is to identify pathway-related modules which are defined as in high-grade osteosarcoma based on topological centralities analysis of networks.
  
  
  
  Co-expression network was constructed by weighted gene co-expression network analysis (WGCNA) based on differentially expressed genes (DEGs). Pathway enrichment analysis was conducted by Kyoto Encyclopedia of Genes and Genomes (KEGG) database to detect pathway enriched genes. Pathway-related modules of pathway enriched genes were mined from the co-expression network. Then topological centralities (degree, closeness, stress and betweenness centrality) analyses for co-expression network and sub-networks were performed to explore hub genes. Validation of hub genes was carried out utilizing reverse transcription-polymerase chain reaction (RT-PCR) assays.
  
  
  
  There were 129 nodes and 1229 edges in co-expression network. We obtained a total of 16 hub genes and 11 pathway-related modules. Module 17 (Bladder cancer module) was the most significant module, which comprising 9 of 16 hub genes and 6 pathway enriched genes, taking intersection elements (CAV1 and CCND1). RT-PCR results showed that both of CAV1 and CCND1 in high-grade osteosarcoma were significantly differentially expressed compared with normal controls.
  
  
  
  This work may contribute to understanding the molecular pathogenesis and provide potential biomarkers for detections and effective therapies of high-grade osteosarcoma.
authors.affiliation: Department of Orthopaedics, People's Hospital of Dongying, Dongying, Shandong Province, China. boning2015@yeah.net.
authors.forename: B
authors.initials: B
authors.lastname: Ning
authors.forename: D-L
authors.initials: DL
authors.lastname: Xu
authors.forename: J-H
authors.initials: JH
authors.lastname: Gao
authors.forename: L-L
authors.initials: LL
authors.lastname: Wang
authors.forename: S-Y
authors.initials: SY
authors.lastname: Yan
authors.forename: S
authors.initials: S
authors.lastname: Cheng
mesh_terms: D015415:Biomarkers; D001859:Bone Neoplasms; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks; D006801:Humans; D012516:Osteosarcoma / Q000378:metabolism*
chemical_list: D015415:Biomarkers