title: Combination effect of cytochrome P450 1A1 gene polymorphisms on uterine leiomyoma: A case-control study.
abstract: Uterine leiomyoma (UL) is an estrogen-dependent neoplasm of the uterus, and estrogen metabolizing enzymes affect its progression. This study aimed to evaluate the association between two single-nucleotide polymorphisms of cytochrome P450 1A1 (CYP1A1) gene and UL risk. The study consisted of 105 patients with UL and 112 healthy women as controls. Ile462Val (A/G) and Asp449Asp (T/C) polymorphisms of CYP1A1 gene were analyzed by DNA sequencing and polymerase chain reaction-restriction fragment length polymorphism methods, respectively. The findings indicated no association between Ile462Val (A/G) and Asp449Asp (T/C) polymorphisms of CYP1A1 gene and UL (p < 0.05). However, the combination effect of TT/AG genotypes of the Asp449Asp (T/C) and Ile462Val (A/G) polymorphisms was associated with 4.3-fold higher risk of UL. In addition, haplotype analysis revealed that TG haplotype of the Asp449Asp (T/C) and Ile462Val (A/G) polymorphisms could increase the UL risk nearly 4.9-fold. Asp449Asp (T/C) and Ile462Val (A/G) polymorphisms of CYP1A1 gene were not associated with UL susceptibility; however, the combination of the TT/AG genotypes and TG haplotype could increase the UL risk.
authors.affiliation: Cellular and Molecular Research Center and Department of Clinical Biochemistry, School of Medicine,Zahedan University of Medical Sciences, Zahedan, Iran. sasalimi@yahoo.com.
authors.forename: Saeedeh
authors.initials: S
authors.lastname: Salimi
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Sajadian
authors.forename: Maryam
authors.initials: M
authors.lastname: Khodamian
authors.forename: Atefeh
authors.initials: A
authors.lastname: Yazdi
authors.forename: Soodabeh
authors.initials: S
authors.lastname: Rezaee
authors.forename: Abbas
authors.initials: A
authors.lastname: Mohammadpour-Gharehbagh
authors.forename: Mojgan
authors.initials: M
authors.lastname: Mokhtari
authors.forename: Minoo
authors.initials: M
authors.lastname: Yaghmaie
mesh_terms: D016022:Case-Control Studies; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D007889:Leiomyoma / Q000453:epidemiology / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics*; D014594:Uterine Neoplasms / Q000453:epidemiology / Q000235:genetics*
chemical_list: C569463:CYP1A1 protein, human; D019363:Cytochrome P-450 CYP1A1