title: Production of Live Offspring from Vitrified-Warmed Oocytes Collected at Metaphase I Stage.
abstract: Vitrification of matured oocytes is widely adopted in human clinics and animal research laboratories. Cryopreservation of immature oocytes, particularly those at metaphase I (MI), remains a challenge. In the present work, mouse MI oocytes denuded of cumulus cells were vitrified and warmed (V/W) either prior to (V/W-BEFORE-IVM, n = 562) or after (V/W-AFTER-IVM, n = 664) in vitro maturation (IVM). Derivative metaphase II (MII) oocytes were then used for intracytoplasmic sperm injection (ICSI). In the control groups, in vivo matured MII oocytes were used freshly (FRESH-MII, n = 517) or after V/W (MII-V/W, n = 617). In vitro and in vivo developmental competencies were compared among groups. Satisfactory blastocyst rates were achieved in V/W-BEFORE-IVM (27.5%) and V/W-AFTER-IVM (32.4%) groups, albeit as expected still lower than those from fresh-MII (56.1%) or MII-V/W (45.6%) oocytes. Similarly, the term development rates from V/W-BEFORE-IVM and V/W-AFTER-IVM were 12.4% and 16.7% respectively, acceptable but lower than those of the fresh-MII (41.2%) and MII-V/W (23.3%) groups. These data demonstrate that oocytes collected at MI stage are amenable to V/W, which can be performed before or after IVM with acceptable development rates including production of healthy pups. These findings provide useful knowledge to researchers and clinical practitioners for preservation and use of the otherwise discarded MI oocytes.
authors.affiliation: Reproductive Biology Associates, Atlanta, Georgia, United States of America.
authors.forename: Ching-Chien
authors.initials: CC
authors.lastname: Chang
authors.affiliation: Institute of Biotechnology, National Taiwan University, Taipei, Taiwan.
authors.forename: Wei-Fang
authors.initials: WF
authors.lastname: Chang
authors.affiliation: Center for Advanced Models for Translational Sciences and Therapeutics, University of Michigan Medical Center, Ann Arbor, Michigan, United States of America.
authors.forename: Jie
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Medicinal Plant Development, Yupintang Traditional Chinese Medicine Foundation, Kaohsiung City, Taiwan.
authors.forename: An-Sheng
authors.initials: AS
authors.lastname: Cheng
authors.affiliation: Institute of Biotechnology, National Taiwan University, Taipei, Taiwan.
authors.forename: Chia-Chun
authors.initials: CC
authors.lastname: Chang
authors.affiliation: Reproductive Biology Associates, Atlanta, Georgia, United States of America.
authors.forename: Zsolt Peter
authors.initials: ZP
authors.lastname: Nagy
authors.affiliation: Department of Animal Science, National Chiayi University, Chiayi City, Taiwan.
authors.forename: Cho-Chen
authors.initials: CC
authors.lastname: Yang
authors.affiliation: Institute of Biotechnology, National Taiwan University, Taipei, Taiwan.|Department of Animal Science and Technology, National Taiwan University, Taipei, Taiwan.
authors.forename: Shih-Torng
authors.initials: ST
authors.lastname: Ding
authors.affiliation: Institute of Biotechnology, National Taiwan University, Taipei, Taiwan.|Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan.|Animal Resource Center, National Taiwan University, Taipei, Taiwan.
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Sung
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002470:Cell Survival; D004624:Embryo Transfer; D047108:Embryonic Development; D005260:Female; D059471:In Vitro Oocyte Maturation Techniques; D008297:Male; D008677:Metaphase; D008810:Mice, Inbred C57BL; D009865:Oocytes / Q000166:cytology*; D013048:Specimen Handling / Q000379:methods*; D020554:Sperm Injections, Intracytoplasmic; D058989:Vitrification