title: Chemiluminescence Resonance Energy Transfer Competitive Immunoassay Employing Hapten-Functionalized Quantum Dots for the Detection of Sulfamethazine.
abstract: We describe a new strategy for using chemiluminescence resonance energy transfer (CRET) by employing hapten-functionalized quantum dots (QDs) in a competitive immunoassay for detection of sulfamethazine (SMZ). Core/multishell QDs were synthesized and modified with phospholipid-PEG. The modified QDs were functionalized with the hapten 4-(4-aminophenyl-sulfonamido)butanoic acid. The CRET-based immunoassay exhibited a limit of detection for SMZ of 9 pg mL(-1), which is >4 orders of magnitude better than a homogeneous fluorescence polarization immunoassay and is 2 orders of magnitude better than a heterogeneous enzyme-linked immunosorbent assay. This strategy represents a simple, reliable, and universal approach for detection of chemical contaminants.
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University , No. 2 Yuanmingyuan West Road, Beijing 100193, China.
authors.forename: Mingfang
authors.initials: M
authors.lastname: Ma
authors.affiliation: Beijing Laboratory for Food Quality and Safety and Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety , No. 2 Yuanmingyuan West Road, Beijing 100193, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Wen
authors.affiliation: Food and Feed Safety Research Unit, Southern Plains Agricultural Research Center, Agricultural Research Service , United States Department of Agriculture, 2881 F&B Road, College Station, Texas 77845, United States.
authors.forename: Ross C
authors.initials: RC
authors.lastname: Beier
authors.affiliation: Faculty of Chemistry, M. V. Lomonosov Moscow State University , Leninsky Gory, Moscow 119992, Russia.
authors.forename: Sergei A
authors.initials: SA
authors.lastname: Eremin
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University , No. 2 Yuanmingyuan West Road, Beijing 100193, China.
authors.forename: Chenglong
authors.initials: C
authors.lastname: Li
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University , No. 2 Yuanmingyuan West Road, Beijing 100193, China.|Beijing Laboratory for Food Quality and Safety and Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety , No. 2 Yuanmingyuan West Road, Beijing 100193, China.
authors.forename: Suxia
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University , No. 2 Yuanmingyuan West Road, Beijing 100193, China.|Beijing Laboratory for Food Quality and Safety and Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety , No. 2 Yuanmingyuan West Road, Beijing 100193, China.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Shen
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University , No. 2 Yuanmingyuan West Road, Beijing 100193, China.|Beijing Laboratory for Food Quality and Safety and Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety , No. 2 Yuanmingyuan West Road, Beijing 100193, China.
authors.forename: Zhanhui
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000890:Anti-Infective Agents / Q000032:analysis; D006241:Haptens / Q000737:chemistry; D007118:Immunoassay / Q000379:methods*; D008163:Luminescent Measurements / Q000379:methods*; D045663:Quantum Dots / Q000737:chemistry*; D013418:Sulfamethazine / Q000032:analysis*
chemical_list: D000890:Anti-Infective Agents; D006241:Haptens; D013418:Sulfamethazine
keywords: chemical contaminant; chemiluminescence resonance energy transfer; hapten functionalization; immunoassay; quantum dots