title: Multicenter Evaluation of the Solana Group A Streptococcus Assay: Comparison with Culture.
abstract: We compared group A Streptococcus (GAS) culture with a rapid helicase-dependent amplification (HDA) method using 1,082 throat swab specimens. The HDA method demonstrated 98.2% sensitivity and 97.2% specificity. GAS prevalence by culture was 20.7%, and it was 22.6% using the HDA method. In 35 min, the HDA method provided rapid, sensitive GAS detection, making culture confirmation unnecessary.
authors.affiliation: Molecular Pathology, Marshfield Labs, Marshfield, Wisconsin, USA uphoff.timothy@marshfieldclinic.org.
authors.forename: Timothy S
authors.initials: TS
authors.lastname: Uphoff
authors.affiliation: Pathology & Laboratory Medicine, Medical College of Wisconsin, Milwaukee, Wisconsin, USA.
authors.forename: Blake W
authors.identifier: 0000-0002-4111-048X
authors.initials: BW
authors.lastname: Buchan
authors.affiliation: Pathology & Laboratory Medicine, Medical College of Wisconsin, Milwaukee, Wisconsin, USA.
authors.forename: Nathan A
authors.initials: NA
authors.lastname: Ledeboer
authors.affiliation: Laboratory Alliance of Central New York, Liverpool, New York, USA.
authors.forename: Paul A
authors.identifier: 0000-0002-5068-1137
authors.initials: PA
authors.lastname: Granato
authors.affiliation: Department of Pathology, University of Utah, Salt Lake City, Utah, USA Primary Children's Medical Center, Salt Lake City, Utah, USA.
authors.forename: Judy A
authors.initials: JA
authors.lastname: Daly
authors.affiliation: Molecular Pathology, Marshfield Labs, Marshfield, Wisconsin, USA.
authors.forename: Tara N
authors.initials: TN
authors.lastname: Marti
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001431:Bacteriological Techniques / Q000379:methods*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D010614:Pharynx / Q000382:microbiology; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D013290:Streptococcal Infections / Q000175:diagnosis*; D013297:Streptococcus pyogenes / Q000302:isolation & purification*; D013997:Time Factors; D055815:Young Adult