title: Oral administration of Japanese sake yeast (Saccharomyces cerevisiae sake) promotes non-rapid eye movement sleep in mice via adenosine A2Areceptors.
abstract: We have demonstrated previously that Japanese sake yeast improves sleep quality in humans. In the present study, we examined the molecular mechanisms of sake yeast to induce sleep by monitoring locomotor activity, electromyogram and electroencephalogram in mice. Oral administration of Japanese sake yeast (100, 200, and 300 mg kg-1) decreased the locomotor activity by 18, 46 and 59% and increased the amount of non-rapid eye movement (NREM) sleep by 1.5-, 2.3- and 2.4-fold (to 37 ± 6, 57 ± 8, and 60 ± 4 min from 25 ± 6 min in the vehicle-administered group, respectively) in a dose-dependent manner for 4 h after oral administration. However, Japanese sake yeast did not change the amount of rapid eye movement (REM) sleep, the electroencephalogram power density during NREM sleep or show any adverse effects, such as rebound of insomnia, during 24 h postadministration and on the next day. An intraperitoneal pretreatment with an adenosine A2Areceptor-selective antagonist, ZM241385 (15 mg kg-1), reduced the amount of NREM sleep of sake yeast-administered mice to the basal level, without changing basal amount of sleep. Conversely, an A1receptor-selective antagonist, 8-cyclopentyltheophylline (10 mg kg-1), did not affect the sleep-promoting effect of Japanese sake yeast. Thus, Japanese sake yeast promotes NREM sleep via activation of adenosine A2Abut not A1receptors.
authors.affiliation: Research and Development Headquarters, Lion Corporation, Odawara, Kanagawa, Japan.|International Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba, Tsukuba, Ibaraki, Japan.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Nakamura
authors.affiliation: Research and Development Headquarters, Lion Corporation, Odawara, Kanagawa, Japan.|International Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba, Tsukuba, Ibaraki, Japan.
authors.forename: Tatsuyuki
authors.initials: T
authors.lastname: Midorikawa
authors.affiliation: Research and Development Headquarters, Lion Corporation, Odawara, Kanagawa, Japan.
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Monoi
authors.affiliation: Research and Development Headquarters, Lion Corporation, Odawara, Kanagawa, Japan.
authors.forename: Eriko
authors.initials: E
authors.lastname: Kimura
authors.affiliation: Research and Development Headquarters, Lion Corporation, Odawara, Kanagawa, Japan.
authors.forename: Ayumi
authors.initials: A
authors.lastname: Murata-Matsuno
authors.affiliation: Research and Development Headquarters, Lion Corporation, Odawara, Kanagawa, Japan.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Sano
authors.affiliation: Research and Development Headquarters, Lion Corporation, Odawara, Kanagawa, Japan.
authors.forename: Kengo
authors.initials: K
authors.lastname: Oka
authors.affiliation: Research and Development Headquarters, Lion Corporation, Odawara, Kanagawa, Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Sugafuji
authors.affiliation: Research and Development Headquarters, Lion Corporation, Odawara, Kanagawa, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Uchiyama
authors.affiliation: Research and Development Headquarters, Lion Corporation, Odawara, Kanagawa, Japan.|Kyoto Prefectural University of Medicine, Kyoto, Japan.
authors.forename: Michiaki
authors.initials: M
authors.lastname: Murakoshi
authors.affiliation: Research and Development Headquarters, Lion Corporation, Odawara, Kanagawa, Japan.|Ritsumeikan Global Innovation Research Organization, Ritsumeikan University, Kusatsu, Shiga, Japan.
authors.forename: Keikichi
authors.initials: K
authors.lastname: Sugiyama
authors.affiliation: Kyoto Prefectural University of Medicine, Kyoto, Japan.
authors.forename: Hoyoku
authors.initials: H
authors.lastname: Nishino
authors.affiliation: International Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba, Tsukuba, Ibaraki, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Urade
mesh_terms: D058916:Adenosine A1 Receptor Antagonists / Q000494:pharmacology; D058917:Adenosine A2 Receptor Antagonists / Q000494:pharmacology; D000284:Administration, Oral; D000434:Alcoholic Beverages / Q000382:microbiology*; D000818:Animals; D004569:Electroencephalography; D004576:Electromyography; D005133:Eye Movements / Q000187:drug effects / Q000502:physiology*; D007564:Japan; D008124:Locomotion / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D043705:Receptor, Adenosine A2A / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000145:classification*; D012890:Sleep / Q000187:drug effects / Q000502:physiology*; D012895:Sleep, REM / Q000502:physiology; D013806:Theophylline / Q000031:analogs & derivatives / Q000494:pharmacology; D013997:Time Factors; D014227:Triazines / Q000494:pharmacology; D014230:Triazoles / Q000494:pharmacology
chemical_list: D058916:Adenosine A1 Receptor Antagonists; D058917:Adenosine A2 Receptor Antagonists; D043705:Receptor, Adenosine A2A; D014227:Triazines; D014230:Triazoles; C097270:ZM 241385; C053907:8-cyclopentyl-1,3-dimethylxanthine; D013806:Theophylline
keywords: adenosine receptor; food supplement; quality of sleep; rodents; vigilance state analysis