title: Long non-coding RNA NEAT1 promotes non-small cell lung cancer progression through regulation of miR-377-3p-E2F3 pathway.
abstract: Recently, the long non-coding RNA (lncRNA) NEAT1 has been identified as an oncogenic gene in multiple cancer types and elevated expression of NEAT1 was tightly linked to tumorigenesis and cancer progression. However, the molecular basis for this observation has not been characterized in progression of non-small cell lung cancer (NSCLC). In our studies, we identified NEAT1 was highly expressed in patients with NSCLC and was a novel regulator of NSCLC progression. Patients whose tumors had high NEAT1 expression had a shorter overall survival than patients whose tumors had low NEAT1 expression. Further, NEAT1 significantly accelerates NSCLC cell growth and metastasis in vitro and tumor growth in vivo. Additionally, by using bioinformatics study and RNA pull down combined with luciferase reporter assays, we demonstrated that NEAT1 functioned as a competing endogenous RNA (ceRNA) for hsa-miR-377-3p, antagonized its functions and led to the de-repression of its endogenous targets E2F3, which was a core oncogene in promoting NSCLC progression. Taken together, these observations imply that the NEAT1 modulated the expression of E2F3 gene by acting as a ceRNA, which may build up the missing link between the regulatory miRNA network and NSCLC progression.
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, Wuhan University, Wuhan, P. R. China.
authors.forename: Chengcao
authors.initials: C
authors.lastname: Sun
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, Wuhan University, Wuhan, P. R. China.|Wuhan Hospital for the Prevention and Treatment of Occupational Diseases, Wuhan, P. R. China.
authors.forename: Shujun
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, Wuhan University, Wuhan, P. R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, Wuhan University, Wuhan, P. R. China.
authors.forename: Yongyong
authors.initials: Y
authors.lastname: Xi
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, Wuhan University, Wuhan, P. R. China.
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, Wuhan University, Wuhan, P. R. China.
authors.forename: Yongyi
authors.initials: Y
authors.lastname: Bi
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, Wuhan University, Wuhan, P. R. China.
authors.forename: Dejia
authors.initials: D
authors.lastname: Li
mesh_terms: D000072283:A549 Cells; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D018450:Disease Progression; D050699:E2F3 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D062085:RNA, Long Noncoding / Q000502:physiology*; D015398:Signal Transduction / Q000235:genetics
chemical_list: D050699:E2F3 Transcription Factor; C098319:E2F3 protein, human; C534063:MIRN377 microRNA, human; D035683:MicroRNAs; C577276:NEAT1 long non-coding RNA, human; D062085:RNA, Long Noncoding
keywords: E2F3; hsa-miRNA-377-3p (miR-377-3p); long non-coding RNA NEAT1 (lncRNA NEAT1); non-small cell lung cancer (NSCLC); tumorigenesis