title: Diagnosis of chronic lymphoproliferative disorders by flow cytometry using four-color combinations for immunophenotyping: A proposal of the brazilian group of flow cytometry (GBCFLUX).
abstract: Multiparametric flow cytometry (MFC) is a powerful tool for the diagnosis of hematological malignancies and has been useful for the classification of chronic lymphoproliferative disorders (CLPD) according to the WHO criteria. Following the purposes of the Brazilian Group of Flow Cytometry (GBCFLUX), the aim of this report was to standardize the minimum requirements to achieve an accurate diagnosis in CLPDs, considering the different economic possibilities of the laboratories in our country. Most laboratories in Brazil work with 4-fluorescence flow cytometers, which is why the GBCFLUX CLPD Committee has proposed 4-color monoclonal antibody (MoAb) panels.
  
  
  
  Panels for screening and diagnosis in B, T and NK lymphoproliferative disorders were developed based on the normal differentiation pathways of these cells and the most frequent phenotypic aberrations. Important markers for prognosis and for minimal residual disease (MRD) evaluation were also included. The MoAb panels presented here were designed based on the diagnostic expertise of the participating laboratories and an extensive literature review.
  
  
  
  The 4-color panels presented to aid in the diagnosis of lymphoproliferative neoplasms by GBCFLUX aim to provide clinical laboratories with a systematic, step-wise, cost-effective, and reproducible approach to obtain an accurate immunophenotypic diagnosis of the most frequent of these disorders. © 2016 International Clinical Cytometry Society.
authors.affiliation: Hospital Das Clínicas Da Faculdade De Medicina Da Universidade De São Paulo, SP, Brazil.
authors.forename: M M
authors.initials: MM
authors.lastname: Sales
authors.affiliation: Centro De Hematologia E Hemoterapia De Santa Catarina - HEMOSC, Brazil.
authors.forename: S I A C P
authors.initials: SIACP
authors.lastname: Ferreira
authors.affiliation: Hospital Amaral Carvalho - Jau, SP, Brazil.
authors.forename: M R V
authors.initials: MRV
authors.lastname: Ikoma
authors.affiliation: Division of Hematology and Flow Cytometry, Fleury Group, São Paulo, SP, Brazil.
authors.forename: A F
authors.initials: AF
authors.lastname: Sandes
authors.affiliation: Unidade De Apoio Diagnóstico, Hospital De Clínicas - UFPR, Brazil.
authors.forename: M P
authors.initials: MP
authors.lastname: Beltrame
authors.affiliation: Hospital Albert Einstein, São Paulo, SP, Brazil.
authors.forename: N S
authors.initials: NS
authors.lastname: Bacal
authors.affiliation: Hospital Das Clínicas Da Faculdade De Medicina Da Universidade De São Paulo, SP, Brazil.
authors.forename: M C A
authors.initials: MCA
authors.lastname: Silva
authors.affiliation: Disciplina De Hematologia Do Departamento De Clínica Médica Da Universidade Federal Do Paraná, PR, Brazil.
authors.forename: M
authors.initials: M
authors.lastname: Malvezzi
authors.affiliation: Universidade Estadual De Campinas, SP, Brazil.
authors.forename: I G H
authors.initials: IGH
authors.lastname: Lorand-Metze
authors.affiliation: Cancer Research Centre (IBMCC, CSIC-USAL), Institute of Biomedical Research of Salamanca (IBSAL), Cytometry Service and Department of Medicine, University of Salamanca, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Orfao
authors.affiliation: Escola Paulista De Medicina, Universidade Federal De São Paulo (EPM-UNIFESP), SP, Brazil.
authors.forename: M
authors.initials: M
authors.lastname: Yamamoto
mesh_terms: D015703:Antigens, CD / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D001938:Brazil; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D019337:Hematologic Neoplasms / Q000473:pathology; D006801:Humans; D016130:Immunophenotyping; D008232:Lymphoproliferative Disorders / Q000175:diagnosis*; D008297:Male; D018365:Neoplasm, Residual / Q000175:diagnosis*; D011379:Prognosis
chemical_list: D015703:Antigens, CD
keywords: GBCFLUX; chronic lymphoproliferative disorders; flow cytometry; lymphoma; monoclonal antibody panel; multiple myeloma