title: [Study on immune status of patients with schistosomiasis japonica in Poyang Lake region III Humoral and cellular immune characteristics between Schistosoma japonicum high and low antibody responders].
abstract: OBJECTIVE
  To explore the immune mechanism of negative results of immune tests of schistosomiasis japonica patients.
  
  
  METHODS
  Totally 142 schistosomiasis patients (positive stool examinations) of Poyang Lake region were tested by ELISA method, and the ROC curve was applied to determine the high and low response of the patients. The levels of cellular immunity and cytokines of high and low responders were compared.
  
  
  RESULTS
  Totally eight schistosomiasis patients were found as low responders. Besides SWAP-IgA (t = -1.588, P > 0.1), the levels of isotype antibodies were significantly lower in the low responders compared with those in the high responders (t = -14.517 to -2.866, all P < 0.05). In the low responders, the propor- tion of CD3⁺T was increased; and the proportions of CD4⁺T, CD8⁺T, CD4⁺CD25⁺Treg, and the ratio of CD4⁺/CD8⁺ were all de- creased, but all of them were not significant (t = -1.72 to 0.974, all P > 0.05) compared with those in the high responders. The differences of IFN-γ and IL-10 between the high and low responders were both not significant (t = -2.426 to 0.216, all P > 0.05).
  
  
  CONCLUSIONS
  There is a significant difference between the high and low responders only in the levels of isotype antibodies. One of the reasons of low response in the immune tests is the much lower antibody level after the antigen-antibody compound is completely formulated.
authors.forename: Zhao-jun
authors.initials: ZJ
authors.lastname: Li
authors.forename: Min
authors.initials: M
authors.lastname: Yuan
authors.forename: Min-jun
authors.initials: MJ
authors.lastname: Ji
authors.forename: Shu-ying
authors.initials: SY
authors.lastname: Xie
authors.forename: Mei-yun
authors.initials: MY
authors.lastname: Lv
authors.forename: Yi-feng
authors.initials: YF
authors.lastname: Li
authors.forename: Wei-ming
authors.initials: WM
authors.lastname: Lan
authors.forename: Yue-min
authors.initials: YM
authors.lastname: Liu
authors.forename: Fei
authors.initials: F
authors.lastname: Hu
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Zeng
authors.forename: Jian-ying
authors.initials: JY
authors.lastname: Li
authors.forename: Dan-dan
authors.initials: DD
authors.lastname: Lin
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000917:Antibody Formation; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000097:blood; D005260:Female; D006801:Humans; D007111:Immunity, Cellular; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D008875:Middle Aged; D012549:Schistosoma japonicum / Q000276:immunology; D012554:Schistosomiasis japonica / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D055815:Young Adult
chemical_list: D000909:Antibodies, Helminth; D016207:Cytokines; D007074:Immunoglobulin G