title: The Role of ADAMTS1 and Versican in Human Myocardial Infarction: A Postmortem Study.
abstract: OBJECTIVE
  To determine the role of a disintegrin and metalloproteinase with thrombospondin type 1 motif (ADAMTS1) and fragmented versican in the myocardial infarction (MI) process in humans and to evaluate the diagnostic efficacy of ADAMTS1 for postmortem diagnosis of MI.
  
  
  METHODS
  Thirty autopsied individuals were allocated into 2 groups, namely, a study group of individuals who died of myocardial infarction (n = 20), and a control group who died of trauma (n = 10). We performed standard immunohistochemical staining on myocardial tissue specimens, studying anti-ADAMTS1, anti-versican, and anti-versican C terminal peptide sequence (DPEAAE) fragments.
  
  
  RESULTS
  Strong, diffuse staining was observed throughout myocardial tissue for ADAMTS1 in the 2 groups. However, in the study group, we observed no expression for ADAMTS1 around fibrotic areas but detected slight staining in coagulative and necrotic zones.
  
  
  CONCLUSIONS
  Similar localizations of ADAMTS and fragmented versican in human heart tissue indicate that versican presumably is cleaved by ADAMTS1. Hence, ADAMTS1 can be regarded as a new marker for postmortem differential diagnosis of MI.
authors.affiliation: Morgue Department, Ankara Regional Office of Council of Forensic Medicine, Ankara, Turkey, drspehlivan@hotmail.com.
authors.forename: Sultan
authors.initials: S
authors.lastname: Pehlivan
authors.affiliation: Department of Forensic Medicine, Faculty of Medicine, Uludag University, Bursa, Turkey.
authors.forename: Murat Serdar
authors.initials: MS
authors.lastname: Gurses
authors.affiliation: Department of Forensic Medicine, Faculty of Medicine, Uludag University, Bursa, Turkey.
authors.forename: Mustafa Numan
authors.initials: MN
authors.lastname: Ural
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Turgut Özal University, Ankara, Turkey.
authors.forename: Sumeyya
authors.initials: S
authors.lastname: Akyol
authors.affiliation: Morgue Department, Bursa Regional Office of Council of Forensic Medicine, Bursa, Turkey.
authors.forename: Filiz
authors.initials: F
authors.lastname: Eren
authors.affiliation: Department of Forensic Medicine, Faculty of Medicine, Uludag University, Bursa, Turkey.
authors.forename: Nursel
authors.initials: N
authors.lastname: Turkmen Inanir
authors.affiliation: Department of Medical Biochemistry, Faculty of Medicine, Turgut Özal University, Ankara, Turkey.
authors.forename: Mehmet Akif
authors.initials: MA
authors.lastname: Gulec
authors.affiliation: Morgue Department, Bursa Regional Office of Council of Forensic Medicine, Bursa, Turkey.
authors.forename: Bulent
authors.initials: B
authors.lastname: Eren
authors.affiliation: Department of Forensic Medicine, Faculty of Medicine, Uludag University, Bursa, Turkey.
authors.forename: Recep
authors.initials: R
authors.lastname: Fedakar
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Turgut Özal University, Ankara, Turkey.
authors.forename: Kadir
authors.initials: K
authors.lastname: Demircan
authors.affiliation: Department of Medical Biochemistry, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
authors.forename: Omer
authors.initials: O
authors.lastname: Akyol
mesh_terms: D000071097:ADAMTS1 Protein / Q000032:analysis*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000175:diagnosis* / Q000473:pathology*; D009206:Myocardium / Q000473:pathology*; D010336:Pathology / Q000379:methods*; D053675:Versicans / Q000032:analysis*; D055815:Young Adult
chemical_list: C508322:VCAN protein, human; D053675:Versicans; D000071097:ADAMTS1 Protein; C103859:ADAMTS1 protein, human
keywords: ADAMTS1; diagnosis; fragmented versican; myocardial infarction; postmortem; versican