title: Combination of different methods for detection of Campylobacter spp. in young children with moderate to severe diarrhea.
abstract: Campylobacter spp. were detected - using culture, ELISA, PCR, and qPCR - among children (0-36months) with moderate to severe diarrhea in Northeastern Brazil. Our data showed that either the qPCR alone or PCR along with ELISA might be an alternative to culture to diagnose Campylobacter due to their enhanced sensitivity.
authors.affiliation: Institute of Biomedicine for Brazilian Semi-Arid & Clinical Research Unit/Center for Global Health, Federal University of Ceara, Fortaleza, CE 60430-270, Brazil. Electronic address: herlicenv@hotmail.com.
authors.forename: Herlice
authors.initials: H
authors.lastname: do Nascimento Veras
authors.affiliation: Institute of Biomedicine for Brazilian Semi-Arid & Clinical Research Unit/Center for Global Health, Federal University of Ceara, Fortaleza, CE 60430-270, Brazil.
authors.forename: Josiane
authors.initials: J
authors.lastname: da Silva Quetz
authors.affiliation: Institute of Biomedicine for Brazilian Semi-Arid & Clinical Research Unit/Center for Global Health, Federal University of Ceara, Fortaleza, CE 60430-270, Brazil.
authors.forename: Ila Fernanda Nunes
authors.initials: IFN
authors.lastname: Lima
authors.affiliation: Institute of Biomedicine for Brazilian Semi-Arid & Clinical Research Unit/Center for Global Health, Federal University of Ceara, Fortaleza, CE 60430-270, Brazil.
authors.forename: Tamara Silva
authors.initials: TS
authors.lastname: Rodrigues
authors.affiliation: Institute of Biomedicine for Brazilian Semi-Arid & Clinical Research Unit/Center for Global Health, Federal University of Ceara, Fortaleza, CE 60430-270, Brazil.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Havt
authors.affiliation: Institute of Biomedicine for Brazilian Semi-Arid & Clinical Research Unit/Center for Global Health, Federal University of Ceara, Fortaleza, CE 60430-270, Brazil.
authors.forename: Luís Carlos
authors.initials: LC
authors.lastname: Rey
authors.affiliation: Science Center/Unit of Statistics, Federal University of Ceara, Fortaleza, CE 60455-760, Brazil.
authors.forename: Rosa Maria Salani
authors.initials: RMS
authors.lastname: Mota
authors.affiliation: Institute of Biomedicine for Brazilian Semi-Arid & Clinical Research Unit/Center for Global Health, Federal University of Ceara, Fortaleza, CE 60430-270, Brazil.
authors.forename: Alberto Melo
authors.initials: AM
authors.lastname: Soares
authors.affiliation: Program for Appropriate Technology in Health (PATH), Seattle, WA 98107, USA.
authors.forename: Mitra
authors.initials: M
authors.lastname: Singhal
authors.affiliation: Program for Appropriate Technology in Health (PATH), Seattle, WA 98107, USA.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Weigl
authors.affiliation: Center for Global Health, University of Virginia, Charlottesville, VA 22908, USA.
authors.forename: Richard
authors.initials: R
authors.lastname: Guerrant
authors.affiliation: Institute of Biomedicine for Brazilian Semi-Arid & Clinical Research Unit/Center for Global Health, Federal University of Ceara, Fortaleza, CE 60430-270, Brazil.
authors.forename: Aldo Angelo Moreira
authors.initials: AAM
authors.lastname: Lima
mesh_terms: D001938:Brazil; D002167:Campylobacter / Q000302:isolation & purification*; D002675:Child, Preschool; D003967:Diarrhea / Q000175:diagnosis* / Q000382:microbiology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D060888:Real-Time Polymerase Chain Reaction; D012680:Sensitivity and Specificity
keywords: Campylobacter spp.; Childhood diarrhea; Disease