title: Study of genetic diversity of killer cell immunoglobulin-like receptor loci in the Tujia ethnic minority.
abstract: The aim of this study was to analyze the genetic profiles of 14 killer cell immunoglobulin-like receptor (KIR) genes and 2 pseudogenes of 124 individuals from Tujia ethnic minority residing in Enshi Tujia and Miao autonomous prefecture of Hubei province of China and investigate the genetic relationships between the Tujia ethnic minority and other reported groups for the first time. Sequence specific primer amplification (PCR-SSP) methods were used to genotype the 14 KIR genes and 2 pseudogenes. The observed carrier frequencies (OF) and the gene frequencies (GF) of the KIR genes were measured. Neighbor-joining (N-J) tree and the principal component analysis (PCA) plot were constructed. All individuals were typed positive for the three framework loci KIR3DL3, 2DL4 and 3DL2, as well as for pseudogene KIR3DP1. The gene frequencies of the other KIR genes ranged from 9% in KIR2DS2 to 98% in KIR2DP1 and KIR3DL1. The present study of the KIR genes may be a powerful tool for enriching the Chinese ethnical gene information resources of the KIR gene pool, as well as for the anthropological research.
authors.affiliation: Medical Genetic Institute of Henan Province, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou 450003, PR China; Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an 710004, PR China.
authors.forename: Hong-Dan
authors.initials: HD
authors.lastname: Wang
authors.affiliation: Department of Urology, The First People's Hospital of Zhengzhou, Zhengzhou 450004, PR China.
authors.forename: Zhan-Qi
authors.initials: ZQ
authors.lastname: Feng
authors.affiliation: Blood Center of Shaanxi Province, Xi'an 710061, PR China.
authors.forename: Chun-Mei
authors.initials: CM
authors.lastname: Shen
authors.affiliation: Medical Genetic Institute of Henan Province, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou 450003, PR China.
authors.forename: Qian-Nan
authors.initials: QN
authors.lastname: Guo
authors.affiliation: Department of Ophthalmology, Xi'an No. 4 Hospital, Guangren Hospital of Xi'an Jiaotong University, Xi'an 710004, PR China.
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Dai
authors.affiliation: Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an 710004, PR China.
authors.forename: Yu-Dang
authors.initials: YD
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an 710004, PR China.
authors.forename: Yu-Xin
authors.initials: YX
authors.lastname: Guo
authors.affiliation: Key Laboratory of Genome Sciences, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Jiang-Wei
authors.initials: JW
authors.lastname: Yan
authors.affiliation: Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an 710004, PR China; Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases, College of Stomatology, Xi'an Jiaotong University, Xi'an 710004, PR China. Electronic address: zhubofeng7372@126.com.
authors.forename: Bo-Feng
authors.initials: BF
authors.lastname: Zhu
authors.affiliation: Henan Red Cross Blood Center, Zhengzhou 450000, PR China. Electronic address: LILYZHL79@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
mesh_terms: D044466:Asian People; D002681:China; D005006:Ethnicity; D005787:Gene Frequency; D005828:Genetics, Population; D005838:Genotype; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology*; D015810:Linkage Disequilibrium; D011110:Polymorphism, Genetic; D011544:Pseudogenes / Q000235:genetics*; D054340:Receptors, KIR / Q000235:genetics*
chemical_list: D054340:Receptors, KIR
keywords: Genetic diversity; Killer cell immunoglobulin-like receptors (KIRs); PCR-SSP; Tujia ethnic minority