title: Development of microsatellite loci of pod mahogany, Afzelia quanzensis (Fabaceae), by Illumina shotgun sequencing, and cross-amplification in A. africana.
abstract: OBJECTIVE
  Microsatellite loci were developed for Afzelia quanzensis (Fabaceae) as a first step toward investigating genetic diversity and population structure of the species in its native range.
  
  
  RESULTS
  Illumina shotgun sequencing was used to generate raw sequence reads, which were searched for potential microsatellite loci. A total of 70 potential microsatellite loci were tested for amplification and polymorphism, and 39 successfully amplified. Of the 39 loci that amplified, 12 were polymorphic while 27 were monomorphic. The 12 polymorphic loci were cross-amplified in A. africana, and eight successfully amplified.
  
  
  CONCLUSIONS
  The 12 polymorphic microsatellite loci can be used for genetic studies of A. quanzensis, which can help determine its conservation status. Eight loci can also be used for genotyping in A. africana.
authors.affiliation: Department of Biological Sciences, University of Illinois at Chicago, 845 West Taylor Street, Chicago, Illinois 60607 USA; Department of Biological Sciences, Bindura University of Science Education, Private Bag 1020, Bindura, Zimbabwe.
authors.forename: Percy
authors.initials: P
authors.lastname: Jinga
authors.affiliation: Department of Biological Sciences, University of Illinois at Chicago, 845 West Taylor Street, Chicago, Illinois 60607 USA.
authors.forename: Jason
authors.initials: J
authors.lastname: Palagi
authors.affiliation: Department of Biological Sciences, University of Illinois at Chicago, 845 West Taylor Street, Chicago, Illinois 60607 USA.
authors.forename: Mary V
authors.initials: MV
authors.lastname: Ashley
keywords: Afzelia africana; Afzelia quanzensis; Fabaceae; Illumina; PAL_FINDER; microsatellite