title: Multi-drug-resistant Enterococcus faecalis among Egyptian patients with urinary tract infection.
abstract: The prevalence of Enterococcus faecalis (E. faecalis) infections among Egyptians with urinary tract infection (UTI), their antimicrobial susceptibility and mechanisms of resistance are under investigated. In this study, 300 urine samples were collected from UTI patients to identify E. faecalis. Antimicrobial susceptibility to 18 antimicrobial agents was tested. The presence of aac(6)-Ie-aph(2)Ia, erm(B) and mef(A/E) genes was examined by PCR. Fifty-seven (19%) isolates were identified as E. faecalis. All isolates were sensitive to teicoplanin and were completely resistant to nalidixic acid, cefotaxime and cefadroxil. Multi-drug-resistant (MDR) was found to be 100% with 45 different antibiotypes. The aac(6)Ia-aph(2)Ia gene was found in 100 and 90% of the isolates resistant to gentamicin at concentrations of 120 and 10 μg, respectively. erm(B) and mef(A/E) genes were present in 92.5% (37/40) and 2.5% (1/40) of erythromycin-resistant isolates, respectively. We conclude that there is a high prevalence of E. faecalis in UTI cases with a 100% MDR rate indicating a serious problem in treating infections by this organism in Egypt.
authors.affiliation: a Faculty of Medicine, Microbiology and Immunology Department , Minia University , Minia , Egypt.|b Faculty of Medicine, Microbiology and Immunology Department , Al-Azhar University , Assiut , Egypt.
authors.forename: Mohammad Z
authors.initials: MZ
authors.lastname: Abdelkareem
authors.affiliation: a Faculty of Medicine, Microbiology and Immunology Department , Minia University , Minia , Egypt.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Sayed
authors.affiliation: a Faculty of Medicine, Microbiology and Immunology Department , Minia University , Minia , Egypt.
authors.forename: Noha A
authors.initials: NA
authors.lastname: Hassuna
authors.affiliation: a Faculty of Medicine, Microbiology and Immunology Department , Minia University , Minia , Egypt.
authors.forename: Mahmoud S
authors.initials: MS
authors.lastname: Mahmoud
authors.affiliation: a Faculty of Medicine, Microbiology and Immunology Department , Minia University , Minia , Egypt.|c Department of Microbiology , College of Pharmacy, Taif University , Taif , Saudi Arabia.
authors.forename: Sayed F
authors.initials: SF
authors.lastname: Abdelwahab
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002648:Child; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D024901:Drug Resistance, Multiple, Bacterial; D004534:Egypt / Q000453:epidemiology; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000235:genetics; D005260:Female; D016908:Gram-Positive Bacterial Infections / Q000188:drug therapy* / Q000453:epidemiology* / Q000382:microbiology; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D015995:Prevalence; D014552:Urinary Tract Infections / Q000150:complications / Q000382:microbiology*; D055815:Young Adult
chemical_list: D000900:Anti-Bacterial Agents
keywords: Antimicrobial resistance; Egypt; Enterococcus faecalis; Multi-drug resistance; Urinary tract infection