title: Cadmium-induced changes in hatchability and in the activity of aminotransaminases and selected lysosomal hydrolases in the blood plasma of Muscovy ducklings (Cairina moschata).
abstract: The aim of this study was to determine the effect of cadmium on Muscovy ducklings (Cairina moschata) based on hatching results and the activity of enzymes in the blood plasma. On day 6 of incubation, hatching eggs were injected into the egg albumen with 50 μl of saline solution containing Cd ions (CdCl2) at concentrations of 0 (control group), 1.3, 4.0, 7.5, 15.0 and 30 μg/egg, using 50 eggs per group. A gradual decrease in hatchability, from 52% in the control to 4% in the highest Cd dose group, was observed, with the LD50 calculated as 8 μg/egg. However, the impact of cadmium on the incidence of malformations of duck embryos has not been proven. Compared to the control group, N-acetyl-β-Dglucosaminidase activity increased by 30-50% (P ≤ 0.05) in the blood serum of ducklings in the groups receiving more than 7.5 μg Cd/egg, whereas an elevated activity of arylsulphatase (by 45%) was observed for a lower dose only (4 μg Cd/egg). A gradual increase in the activity of alanine and aspartate aminotransferases was observed (P ≤ 0.05), starting from the lowest exposure of 1.3 μg Cd/egg, by 155% and 53%, respectively. In conclusion, the results prove the dosedependent toxic impact of cadmium on embryogenesis and on the studied blood plasma enzyme activities of ducklings.
authors.affiliation: Department of Chemistry and Food Toxicology, University of Rzeszów , Ćwiklińskiej 2, 35-601 Rzeszów , Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Dżugan
authors.affiliation: Department of Veterinary and Animal Reproduction and Welfare, University of Agriculture in Krakow , Kraków, , Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Lis
mesh_terms: D000410:Alanine Transaminase / Q000097:blood / Q000378:metabolism*; D000818:Animals; D002104:Cadmium / Q000008:administration & dosage / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004372:Ducks / Q000097:blood*; D006867:Hydrolases / Q000097:blood / Q000378:metabolism*; D008247:Lysosomes / Q000201:enzymology*; D010063:Ovum; D014874:Water Pollutants, Chemical / Q000008:administration & dosage / Q000633:toxicity*
chemical_list: D014874:Water Pollutants, Chemical; D002104:Cadmium; D000410:Alanine Transaminase; D006867:Hydrolases
keywords: Muscovy duck; aminotransferases; cadmium; embryogenesis; lysosomal hydrolases