title: From the Cover: Prenatal Nicotinic Exposure Attenuates Respiratory Chemoreflexes Associated With Downregulation of Tyrosine Hydroxylase and Neurokinin 1 Receptor in Rat Pup Carotid Body.
abstract: Maternal cigarette smoke is the major risk of sudden infant death syndrome (SIDS). A depressed ventilatory response to hypoxia (HVR) and hypercapnia (HCVR) is thought to be responsible for the pathogenesis of SIDS and the carotid body is critically involved in these responses. We have recently reported that prenatal nicotinic exposure (PNE) over the full gestation induces depressed HVR in rat pups. Here, we asked whether PNE (1) depressed not only HVR but also HCVR that were dependent on the carotid body, (2) affected some important receptors and neurochemicals expressed in the carotid body, such as tyrosine hydroxylase (TH), neurokinin-1 receptor (NK1R), and α7 nicotinic acetylcholine receptor (α7nAChR), and (3) blunted the ventilatory responses to activation of these receptors. To this end, HVR and HCVR in Ctrl and PNE pups were measured with plethysmography before and after carotid body ablation (Series I), mRNA expression and/or immunoreactivity (IR) of TH, NK1R, and α7nAChR in the carotid body were examined by RT-PCR and immunohistochemistry (Series II), and the ventilatory responses were tested before and after intracarotid injection of substance P (NK1R agonist) and AR-R17779 (α7nAChR agonist) (Series III). Our results showed that PNE (1) significantly depressed both HVR and HCVR and these depressions were abolished by carotid body ablation, (2) reduced the relative population of glomus cells, mRNA NK1R, and α7nAChR and IR of NK1R and TH in the carotid body, and (3) decreased ventilatory responses to intracarotid injection of substance P or AR-R17779. These results suggest that PNE acting via the carotid body could strikingly blunt HVR and HCVR, likely through downregulating TH and NK1R.
authors.affiliation: *Lovelace Respiratory Research Institute, Pathophysiology Program, Albuquerque, New Mexico 87108.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
authors.affiliation: *Lovelace Respiratory Research Institute, Pathophysiology Program, Albuquerque, New Mexico 87108.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Zhuang
authors.affiliation: *Lovelace Respiratory Research Institute, Pathophysiology Program, Albuquerque, New Mexico 87108.
authors.forename: Xiuping
authors.initials: X
authors.lastname: Gao
authors.affiliation: *Lovelace Respiratory Research Institute, Pathophysiology Program, Albuquerque, New Mexico 87108.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Ye
authors.affiliation: Department of Physiology, University of Kentucky Medical Center, Lexington, Kentucky 40536.
authors.forename: Lu-Yuan
authors.initials: LY
authors.lastname: Lee
authors.affiliation: *Lovelace Respiratory Research Institute, Pathophysiology Program, Albuquerque, New Mexico 87108 fxu@lrri.org.
authors.forename: Fadi
authors.initials: F
authors.lastname: Xu
mesh_terms: D000818:Animals; D002344:Carotid Body / Q000187:drug effects* / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects*; D005260:Female; D008297:Male; D009538:Nicotine / Q000008:administration & dosage* / Q000494:pharmacology; D010991:Plethysmography; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D018040:Receptors, Neurokinin-1 / Q000378:metabolism*; D012137:Respiratory System / Q000187:drug effects*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*; D064569:alpha7 Nicotinic Acetylcholine Receptor / Q000378:metabolism
chemical_list: D018040:Receptors, Neurokinin-1; D064569:alpha7 Nicotinic Acetylcholine Receptor; D009538:Nicotine; D014446:Tyrosine 3-Monooxygenase
keywords: SIDS; carotid body ablation; dopamine D2 receptor; glomus cells; maternal cigarette smoke; ventilatory response to hypoxia and hypercapnia.