title: Advancements in Next-Generation Sequencing.
abstract: The term next-generation sequencing is almost a decade old, but it remains the colloquial way to describe highly parallel or high-output sequencing methods that produce data at or beyond the genome scale. Since the introduction of these technologies, the number of applications and methods that leverage the power of genome-scale sequencing has increased at an exponential pace. This review highlights recent concepts, technologies, and methods from next-generation sequencing to illustrate the breadth and depth of the applications and research areas that are driving progress in genomics.
authors.affiliation: HudsonAlpha Institute for Biotechnology, Huntsville, Alabama 35806; email: slevy@hudsonalpha.org , rmyers@hudsonalpha.org.
authors.forename: Shawn E
authors.initials: SE
authors.lastname: Levy
authors.affiliation: HudsonAlpha Institute for Biotechnology, Huntsville, Alabama 35806; email: slevy@hudsonalpha.org , rmyers@hudsonalpha.org.
authors.forename: Richard M
authors.initials: RM
authors.lastname: Myers
mesh_terms: D015894:Genome, Human / Q000235:genetics*; D023281:Genomics / Q000639:trends*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods / Q000639:trends*; D006801:Humans; D017422:Sequence Analysis, DNA / Q000379:methods
keywords: exome; sequencing; whole genome