title: Intermedin A, a New Labdane Diterpene Isolated from Alpinia intermedia, Prolonged the Survival Time of P-388D1 Tumor-Bearing CDF1 Mice.
abstract: Eight ethanolic extracts of indigenous Taiwanese plants of the genusAlpiniawere tested for tumor cytotoxicity against AGS, Hep G2, HeLa, KB, and HL-60 cells. Among the 50 % and 95 % EtOH extracts of eightAlpiniaspecies, the cytotoxic effects ofAlpinia intermedialeaves were the strongest. When the leaf extract ofA. intermediawas partitioned usingn-hexane and aqueous solvents, then-hexane layer showed a greater cytotoxic effect and could prolong the survival time of P-388D1tumor-bearing CDF1 mice. Two new labdane diterpene derivatives, intermedin A (1) and intermedin B (2), and coronarin E (3) were isolated from then-hexane layer ofA. intermedia. Intermedin A induced apoptosis in HL-60 cells at 30 µg/mL and significantly prolonged the survival time of P-388D1tumor-bearing CDF1mice by 48.7 % at 20 mg/kg of body weight. We suggest that intermedin A is a major compound ofA. intermediaand has a cytotoxic effect on HL-60 and P-388D1cells.
authors.affiliation: Department of Microbiology, Immunology and Biopharmaceuticals, College of Life Sciences, National Chiayi University, Chiayi, Taiwan.
authors.forename: Lih-Geeng
authors.initials: LG
authors.lastname: Chen
authors.affiliation: Graduate Institute of Pharmacognosy Science, School of Pharmacy, Pharmacy of College, Taipei Medical University, Taipei, Taiwan.
authors.forename: Pei-Jung
authors.initials: PJ
authors.lastname: Su
authors.affiliation: School of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei, Taiwan.
authors.forename: Po-Wei
authors.initials: PW
authors.lastname: Tsai
authors.affiliation: School of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei, Taiwan.
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: Yang
authors.affiliation: Graduate Institute of Pharmacognosy Science, School of Pharmacy, Pharmacy of College, Taipei Medical University, Taipei, Taiwan.
authors.forename: Ching-Chiung
authors.initials: CC
authors.lastname: Wang
mesh_terms: D032429:Alpinia / Q000737:chemistry*; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000431:Ethanol; D006801:Humans; D017705:Lignans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D015394:Molecular Structure; D009374:Neoplasms, Experimental / Q000188:drug therapy / Q000401:mortality; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; D017705:Lignans; D010936:Plant Extracts; D012717:Sesquiterpenes; C482830:coronarin E; C531426:intermedin A; C531427:intermedin B; C433017:labdane; D000431:Ethanol