title: An experimental study on effects of pyrrolidine dithiocarbamate on ischemia-reperfusion injury in testis.
abstract: BACKGROUND
  The aim of this experimental study was to investigate the histopathological and biochemical effects of pyrrolidine dithiocarbamate, an antioxidant and inhibitor of NF-kβ, on ischemiareperfusion injury in rats.
  
  
  METHODS
  A total of 21 male Wistar-Albino rats were randomly distributed into three groups as sham group (Group 1), ischemia-reperfusion (I/R) group (Group 2) and I/R with pyrrolidine dithiocarbamate (PDTC) group (Group 3). Left testicles of rats in Groups 2 and 3 underwent testicular torsion of 720° for four hours and 100 mg/kg of PDTC was administered intraperitoneally prior to detorsion in Group 3. An hour after detorsion process, left orchiectomies were performed and 5 ml of intracardiac blood samples were drawn from rats in all three groups. Histopathological examination of testis tissues performed and measurement of superoxide dismutase (SOD) and malondialdehyde (MDA) levels in blood samples were taken.
  
  
  RESULTS
  Elevated levels of MDA and decreased SOD activity, together with decreased Johnson tubular biopsy scores consistent with I/R injury were observed in Group 2 (p<0.05). Group 1 and Group 3 were similar in terms of MDA levels, SOD activity, and Johnson scores (p>0.05).
  
  
  CONCLUSIONS
  Our results indicated that PDTC may have beneficial effects for alleviation of I/R injury in testicular tissue in rats. Understanding the underlying mechanisms and exploration of its diagnostic and therapeutic potential requires further randomized, controlled trials on a larger scale.
authors.affiliation: Abant Izzet Baysal University, School of Medicine, Turkey.
authors.forename: Eray
authors.initials: E
authors.lastname: Kemahli
authors.affiliation: Abant Izzet Baysal University, School of Medicine, Turkey.
authors.forename: Mevlüt
authors.initials: M
authors.lastname: Yildiz
authors.affiliation: Abant Izzet Baysal University, School of Medicine, Turkey.
authors.forename: Tülin
authors.initials: T
authors.lastname: Firat
authors.affiliation: Abant Izzet Baysal University, School of Medicine, Turkey.
authors.forename: Mehmet Emin
authors.initials: ME
authors.lastname: Özyalvaçli
authors.affiliation: Abant Izzet Baysal University, School of Medicine, Turkey.
authors.forename: Uğur
authors.initials: U
authors.lastname: Üyetürk
authors.affiliation: Abant Izzet Baysal University, School of Medicine, Turkey.
authors.forename: Burak
authors.initials: B
authors.lastname: Yilmaz
authors.affiliation: Abant Izzet Baysal University, School of Medicine, Turkey.
authors.forename: Adnan
authors.initials: A
authors.lastname: Gücük