title: Derivation of the clinical grade human embryonic stem cell line RCe020-a (RC-16).
abstract: The human embryonic stem cell line RCe020-A (RC-16) was derived under quality assured compliance with UK regulation, European Union Directives and International guidance for tissue procurement, processing and storage according to Good Manufacturing Practice (GMP) standards. The cell line was derived from a failed to fertilise oocyte voluntarily donated as unsuitable or surplus to fertility requirements following informed consent. RCe020-A (RC-16) shows normal pluripotency marker expression and differentiates to mesoderm and potentially ectoderm in vitro. It has an abnormal 47XX, +14, i(20)(q10) female karyotype and microsatellite PCR identity, HLA and blood group typing data is available.
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh EH16 4UX, UK; Centre for Clinical Brain Sciences, University of Edinburgh, UK; MRC Centre for Regenerative Medicine, University of Edinburgh, UK.
authors.forename: P A
authors.initials: PA
authors.lastname: De Sousa
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh EH16 4UX, UK.
authors.forename: B J
authors.initials: BJ
authors.lastname: Tye
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh EH16 4UX, UK.
authors.forename: K
authors.initials: K
authors.lastname: Bruce
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh EH16 4UX, UK.
authors.forename: P
authors.initials: P
authors.lastname: Dand
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh EH16 4UX, UK.
authors.forename: G
authors.initials: G
authors.lastname: Russell
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh EH16 4UX, UK.
authors.forename: D M
authors.initials: DM
authors.lastname: Collins
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh EH16 4UX, UK.
authors.forename: A
authors.initials: A
authors.lastname: Greenshields
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh EH16 4UX, UK.
authors.forename: K
authors.initials: K
authors.lastname: McDonald
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh EH16 4UX, UK.
authors.forename: H
authors.initials: H
authors.lastname: Bradburn
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh EH16 4UX, UK.
authors.forename: A
authors.initials: A
authors.lastname: Laurie
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh EH16 4UX, UK.
authors.forename: J M
authors.initials: JM
authors.lastname: Downie
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh EH16 4UX, UK.
authors.forename: M
authors.initials: M
authors.lastname: Bateman
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh EH16 4UX, UK.
authors.forename: A
authors.initials: A
authors.lastname: Courtney
mesh_terms: D001755:Blastocyst / Q000166:cytology*; D001789:Blood Group Antigens / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D005260:Female; D005838:Genotype; D006650:Histocompatibility Testing; D000066449:Human Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D059785:Karyotype; D018895:Microsatellite Repeats / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D001789:Blood Group Antigens; D014157:Transcription Factors