title: The Effects of LW-AFC on Intestinal Microbiome in Senescence-Accelerated Mouse Prone 8 Strain, a Mouse Model of Alzheimer's Disease.
abstract: Microbes have deserved broader attention as causal factors in Alzheimer's disease (AD), a neurodegenerative disorder. The senescence-accelerated mouse prone 8 (SAMP8) strain, a spontaneous mice of accelerated aging, are considered a robust model for sporadic AD. LW-AFC, an herbal medicine, was prepared from LiuweiDihuang decoction, which is a classical traditional Chinese medicine prescription. Here, we showed that the treatment of LW-AFC improved cognitive impairments of SAMP8 mice, including spatial learning and memory ability, active avoidance response, and object recognition memory capability. Our data indicated that there were significantly 8 increased and 12 decreased operational taxonomic units (OTUs) in the gut microbiota of SAMP8 mice compared with senescence accelerated mouse resistant 1 (SAMR1) strains, the control of SAMP8 mice. The treatment of LW-AFC altered 22 (16 increased and 6 decreased) OTUs in SAMP8 mice and among them, 15 OTUs could be reversed by LW-AFC treatment resulting in a microbial composition similar to that of SAMR1 mice. We further showed that there were 7 (3 negative and 4 positive correlation) OTUs significantly correlated with all the three types of cognitive abilities, at the order level, including Bacteroidales, Clostridiales, Desulfovibrionales, CW040, and two unclassified orders. LW-AFC had influences on bacterial taxa correlated with the abilities of learning and memory in SAMP8 mice and restored them to SAMR1 mice. Our results indicate that the effects of LW-AFC on improving cognitive impairments of SAMP8 mice might be via modulating intestinal microbiome and LW-AFC could be used as a potential anti-AD agent.
authors.affiliation: Department of TCM and Neuroimmunopharmacology, Beijing Institute of Pharmacology and Toxicology, Beijing, China.|State Key Laboratory of Toxicology and Medical Countermeasures, Beijing, China.
authors.forename: Jianhui
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Biotechnology, Beijing Institute of Radiation Medicine, Beijing, China.
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Ye
authors.affiliation: Department of TCM and Neuroimmunopharmacology, Beijing Institute of Pharmacology and Toxicology, Beijing, China.|State Key Laboratory of Toxicology and Medical Countermeasures, Beijing, China.
authors.forename: Xiaorui
authors.initials: X
authors.lastname: Cheng
authors.affiliation: Department of TCM and Neuroimmunopharmacology, Beijing Institute of Pharmacology and Toxicology, Beijing, China.|State Key Laboratory of Toxicology and Medical Countermeasures, Beijing, China.
authors.forename: Xiaorui
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of TCM and Neuroimmunopharmacology, Beijing Institute of Pharmacology and Toxicology, Beijing, China.|State Key Laboratory of Toxicology and Medical Countermeasures, Beijing, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of TCM and Neuroimmunopharmacology, Beijing Institute of Pharmacology and Toxicology, Beijing, China.|State Key Laboratory of Toxicology and Medical Countermeasures, Beijing, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
authors.affiliation: Department of Biotechnology, Beijing Institute of Radiation Medicine, Beijing, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Ni
authors.affiliation: Department of TCM and Neuroimmunopharmacology, Beijing Institute of Pharmacology and Toxicology, Beijing, China.
authors.forename: Shanyi
authors.initials: S
authors.lastname: Qiao
authors.affiliation: Department of TCM and Neuroimmunopharmacology, Beijing Institute of Pharmacology and Toxicology, Beijing, China.|State Key Laboratory of Toxicology and Medical Countermeasures, Beijing, China.
authors.forename: Wenxia
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Department of TCM and Neuroimmunopharmacology, Beijing Institute of Pharmacology and Toxicology, Beijing, China.|State Key Laboratory of Toxicology and Medical Countermeasures, Beijing, China.
authors.forename: Yongxiang
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000544:Alzheimer Disease / Q000150:complications / Q000188:drug therapy* / Q000235:genetics; D000818:Animals; D003072:Cognition Disorders / Q000188:drug therapy* / Q000209:etiology; D003213:Conditioning, Psychological / Q000187:drug effects; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D000069196:Gastrointestinal Microbiome / Q000187:drug effects*; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D008517:Phytotherapy / Q000379:methods; D025341:Principal Component Analysis
chemical_list: D004365:Drugs, Chinese Herbal; C042579:Liuwei Dihuang Decoction
keywords: Alzheimer’s disease; LW-AFC; microbiome; senescence-accelerated mouse prone 8 strain; traditional Chinese medicine