title: Upregulation of AEG-1 Involves in Schwann Cell Proliferation and Migration After Sciatic Nerve Crush.
abstract: Astrocyte-elevated gene-1 (AEG-1), also known as metadherin (MTDH) and lysine-rich CEACAM1 coisolated (LYRIC), has emerged as an important oncogene that regulates key cellular processes including apoptosis, migration, invasion, proliferation, and differentiation. It was reported that AEG-1 enhanced breast cancer cells migration in a NF-κB-dependent manner. Also, AEG-1 contributed cell proliferation through the PI3K-Akt/cyclin pathway. Besides, AEG-1 is implicated in diverse physiological and pathological diseases of the central nervous system (CNS), such as brain tumors, neuroblastomas, neurodegeneration, and neuronal development. However, the role of AEG-1 in the process of peripheral nervous regeneration after injury remains virtually unknown. In this study, we investigated the spatiotemporal expression of AEG-1 in a rat sciatic nerve crush model. At its peak expression, AEG-1 was expressed mainly in Schwann cells of the distal sciatic nerve segment from injury, but had few colocalizations in axons. Besides, the peak expression of AEG-1 was in parallel with proliferating cell nuclear antigen (PCNA). In vitro, we detected the increased expression of AEG-1 during the process of tumor necrosis factor α (TNF-α)-induced Schwann cell proliferation. Meanwhile, interference of AEG-1 inhibited both proliferation and migration of Schwann cells. In conclusion, we speculated that AEG-1 is involved in biochemical and physiological responses after sciatic nerve crush (SNC).
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China. wyh15152885532@163.com.
authors.forename: Youhua
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Weidong
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Undergraduate Student of Medical School of Nantong University, Class 125, 19 Qi-Xiu Road, Nantong, 226001, Jiangsu Province, China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Undergraduate Student of Medical Imaging Department of Medical School of Nantong University, Class 151, 19 Qi-Xiu Road, Nantong, 226001, Jiangsu Province, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Xingxing
authors.initials: X
authors.lastname: Mao
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Xinbao
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Youhua
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002465:Cell Movement; D049109:Cell Proliferation; D008297:Male; D009409:Nerve Crush; D059348:Peripheral Nerve Injuries / Q000378:metabolism*; D016601:RNA-Binding Proteins; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D012584:Sciatic Nerve / Q000166:cytology / Q000293:injuries / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D015854:Up-Regulation
chemical_list: D002352:Carrier Proteins; C493282:Mtdh protein, rat; D016601:RNA-Binding Proteins; D014409:Tumor Necrosis Factor-alpha
keywords: AEG-1; Migration; Proliferation; Schwann cell; Sciatic nerve crush