title: Surface-Transfer Mass Spectrometry Imaging of Renal Tissue on Gold Nanoparticle Enhanced Target.
abstract: Renal cell carcinoma (RCC) accounts for several percent of all adult malignant tumor cases and is directly associated with over 120 thousand death cases worldwide annually. Therefore, there is a need for cancer biomarker tests and methods capable of discriminating between normal and malignant tissue. It is demonstrated that gold nanoparticle enhanced target (AuNPET), a nanoparticle-based, surface-assisted laser desorption/ionization (SALDI)-type mass spectrometric method for analysis and imaging, can differentiate between normal and cancerous renal tissue. Diglyceride DG(18:1/20:0)-sodium adduct and protonated octadecanamide ions were found to have greatly elevated intensities in cancerous part of analyzed tissue specimen. Compounds responsible for mentioned ions formation were pointed out as a potential clear cell RCC biomarkers. Their biological properties and localization on the tissue surface are also discussed. Potential application of presented results may also facilitate clinical decision making during surgery for large renal masses.
authors.affiliation: Faculty of Chemistry, Rzeszów University of Technology , 35-959 Rzeszów, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Nizioł
authors.affiliation: Department of General and Oncological Urology, Rzeszow City Hospital , 35-241 Rzeszow, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Ossoliński
authors.affiliation: Department of General and Oncological Urology, Rzeszow City Hospital , 35-241 Rzeszow, Poland.
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Ossoliński
authors.affiliation: Department of General and Oncological Urology, Rzeszow City Hospital , 35-241 Rzeszow, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Ossolińska
authors.affiliation: Department of Microbiology and Plant Biology, The University of Oklahoma , Norman, Oklahoma 73019, United States.
authors.forename: Vincent
authors.initials: V
authors.lastname: Bonifay
authors.affiliation: Faculty of Chemistry, Rzeszów University of Technology , 35-959 Rzeszów, Poland.
authors.forename: Justyna
authors.initials: J
authors.lastname: Sekuła
authors.affiliation: Department of General and Oncological Urology, Rzeszow City Hospital , 35-241 Rzeszow, Poland.
authors.forename: Zygmunt
authors.initials: Z
authors.lastname: Dobrowolski
authors.affiliation: Department of Microbiology and Plant Biology, The University of Oklahoma , Norman, Oklahoma 73019, United States.
authors.forename: Jan
authors.initials: J
authors.lastname: Sunner
authors.affiliation: Department of Microbiology and Plant Biology, The University of Oklahoma , Norman, Oklahoma 73019, United States.
authors.forename: Iwona
authors.initials: I
authors.lastname: Beech
authors.affiliation: Faculty of Chemistry, Rzeszów University of Technology , 35-959 Rzeszów, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Ruman
mesh_terms: D000473:Alkanes / Q000737:chemistry; D000577:Amides / Q000737:chemistry; D014408:Biomarkers, Tumor / Q000032:analysis / Q000737:chemistry; D002292:Carcinoma, Renal Cell / Q000737:chemistry / Q000175:diagnosis* / Q000000981:diagnostic imaging; D004075:Diglycerides / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007668:Kidney / Q000737:chemistry* / Q000000981:diagnostic imaging / Q000473:pathology; D007680:Kidney Neoplasms / Q000737:chemistry / Q000175:diagnosis* / Q000000981:diagnostic imaging; D053768:Metal Nanoparticles / Q000737:chemistry*; D012964:Sodium / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: C011439:1,2-diacylglycerol; D000473:Alkanes; D000577:Amides; D014408:Biomarkers, Tumor; D004075:Diglycerides; D006046:Gold; D012964:Sodium; C022883:octadecane