title: A new sensitive indicator cell line reveals cross-transactivation of the viral LTR by gorilla and chimpanzee simian foamy viruses.
abstract: The majority of currently identified simian foamy virus (SFV)-infected Cameroonian and Gabonese individuals harbor SFV from the gorilla lineage. We constructed an indicator cell line for the quantification of gorilla SFVs, in which the U3 sequence of a gorilla SFV directs the expression of the β-galactosidase protein. The gorilla foamy virus activated β-galactosidase (GFAB) cells efficiently quantified two zoonotic primary gorilla isolates and SFVs from three chimpanzee subspecies. Primary gorilla SFVs replicated more slowly and at lower levels than primary chimpanzee SFVs. Analysis of previously described motifs of Tas proteins and U3 LTRs involved in viral gene synthesis revealed conservation of such motifs in Tas proteins from gorilla and chimpanzee SFVs, but little sequence homology in the LTR regions previously shown to interact with viral and cellular factors.
authors.affiliation: Unité d'épidémiologie et physiopathologie des virus oncogènes, Institut Pasteur, 28 rue du Dr Roux, 75015 Paris, France; UMR CNRS 3569, Institut Pasteur, Paris 75015, France; Sorbonne Paris Cité, Cellule Pasteur, Université Paris Diderot, Institut Pasteur, 75015 Paris, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Lambert
authors.affiliation: Unité d'épidémiologie et physiopathologie des virus oncogènes, Institut Pasteur, 28 rue du Dr Roux, 75015 Paris, France.
authors.forename: Réjane
authors.initials: R
authors.lastname: Rua
authors.affiliation: Unité d'épidémiologie et physiopathologie des virus oncogènes, Institut Pasteur, 28 rue du Dr Roux, 75015 Paris, France; UMR CNRS 3569, Institut Pasteur, Paris 75015, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Gessain
authors.affiliation: Unité d'épidémiologie et physiopathologie des virus oncogènes, Institut Pasteur, 28 rue du Dr Roux, 75015 Paris, France; UMR CNRS 3569, Institut Pasteur, Paris 75015, France. Electronic address: florence.buseyne@pasteur.fr.
authors.forename: Florence
authors.initials: F
authors.lastname: Buseyne
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D002478:Cells, Cultured; D006224:Cricetinae; D015870:Gene Expression; D017930:Genes, Reporter; D005822:Genetic Vectors; D006071:Gorilla gorilla; D059372:Nucleotide Motifs; D002679:Pan troglodytes; D011485:Protein Binding; D053150:Simian foamy virus / Q000502:physiology*; D013045:Species Specificity; D020079:Terminal Repeat Sequences; D015533:Transcriptional Activation
keywords: Foamy viruses; Gorilla; Long terminal repeat; Viral transcription; Zoonotic infection