title: Surgical eye spears for saliva collection and secretory immunoglobulin A measurements.
abstract: We examined the use of surgical eye spears for saliva collection and recovery of secretory immunoglobulin A (s-IgA). We established a protocol for recovering s-IgA at concentrations equivalent to those present in the control samples (untreated by surgical eye spears). Four different types of eye spears were submerged in the salivary fluid. Saliva was recovered from each spear by centrifugation. Novel ultra-sensitive enzyme-linked immunosorbent assay was used to determine s-IgA concentration in each sample. The results revealed that Weck-Cel eye spears recovered s-IgA at concentrations not significantly different from s-IgA concentrations in control samples as long as the appropriate protocol was followed. We demonstrated that the use of surgical eye spears for saliva collection is a viable method for recovering s-IgA. (J Oral Sci 58, 205-210, 2016).
authors.affiliation: Jaffe Food Allergy Institute, Pediatric Allergy and Immunology, Icahn School of Medicine at Mount Sinai.
authors.forename: George N
authors.initials: GN
authors.lastname: Konstantinou
authors.forename: Christina M
authors.initials: CM
authors.lastname: Cherny
authors.forename: Anna
authors.initials: A
authors.lastname: Nowak-Węgrzyn
mesh_terms: D000328:Adult; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000378:metabolism*; D008297:Male; D012463:Saliva / Q000378:metabolism; D013048:Specimen Handling
chemical_list: D007071:Immunoglobulin A, Secretory