title: Episomal-based generation of an iPS cell line from human fetal foreskin fibroblasts.
abstract: Human fetal foreskin fibroblasts (HFF1) were used to generate the iPSC line epiHFF1-B1 employing a combination of three episomal-based plasmids expressing OCT4, SOX2, NANOG, LIN28, c-MYC, and KLF4. Pluripotency was confirmed both in vivo and in vitro. The transcriptome profile of epiHFF1-B1 and the human embryonic stem cell line-H1 have a pearson correlation of 0.936.
authors.affiliation: Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany; Max Planck Institute for Molecular Genetics, 14195 Berlin, Germany; Institute of Biology, Humboldt University, 10099 Berlin, Germany.
authors.forename: Peggy
authors.initials: P
authors.lastname: Matz
authors.affiliation: Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany; Max Planck Institute for Molecular Genetics, 14195 Berlin, Germany.
authors.forename: James
authors.initials: J
authors.lastname: Adjaye
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D016172:DNA Fingerprinting; D005347:Fibroblasts / Q000166:cytology*; D052816:Foreskin / Q000166:cytology*; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D007621:Karyotyping; D000090062:Kruppel-Like Factor 4; D008297:Male; D016513:Mice, SCID; D020411:Oligonucleotide Array Sequence Analysis; D010957:Plasmids / Q000378:metabolism*