title: Impact of container material on the development of Aedes aegypti larvae at different temperatures.
abstract: OBJECTIVE
  Aedes aegypti, the primary vector of dengue generally breeds in intradomestic and peridomestic containers made up of different materials, i.e. plastic, iron, rubber, earthen material etc. The material of container is likely to affect the temperature of water in container with variation in environmental temperature. The present study was aimed to determine the effect of different container materials on larval development of Ae. aegypti at different temperatures.
  
  
  METHODS
  Newly hatched I instar larvae (2-4 h old) were used in the study and experiments were conducted using three different containers made up of plastic, iron and earthen material. Three replicates for each type of container at 22, 26, 30, 34, 38, 40, and 42°C were placed in environmental chamber for the development of larvae.
  
  
  RESULTS
  At temperatures >22°C, 50% pupation was completed in earthen pot within 4.3±0.6 to 6.3±0.6 days followed by plastic containers (5±0 to 8±0 days) and iron containers (6±0 to 9±0 days). Developmental time for 50% pupation in the three containers differed significantly (p < 0.05) at all the experimental temperature ranges. A significant variation was found in the temperature of environmental chamber and the temperature of water in three types of containers (p < 0.05). The difference in the temperatures of water in different containers resulted in significant variations in the developmental period of larvae. More than 35°C temperature of water was found inimical for pupal development.
  
  
  CONCLUSIONS
  The results revealed the variation in temperature of water in different types of containers depending on the material of container, affecting duration of larval development. As the larval development was faster in earthen pot as compared to plastic and iron containers, community should be discouraged for storing the water in earthen pots. However, in view of containers of different materials used by the community in different temperature zones in the country, further studies are required for devising area-specific preventive measures for Aedes breeding.
authors.affiliation: National Institute of Malaria Research (ICMR), New Delhi, India.
authors.forename: Gaurav
authors.initials: G
authors.lastname: Kumar
authors.affiliation: National Institute of Malaria Research (ICMR), New Delhi, India.
authors.forename: R K
authors.initials: RK
authors.lastname: Singh
authors.affiliation: Department of Biotechnology, Kumaun University, Nainital, Uttarakhand, India.
authors.forename: Veena
authors.initials: V
authors.lastname: Pande
authors.affiliation: National Institute of Malaria Research (ICMR), New Delhi, India.
authors.forename: R C
authors.initials: RC
authors.lastname: Dhiman
mesh_terms: D000330:Aedes / Q000254:growth & development* / Q000528:radiation effects*; D000818:Animals; D017753:Ecosystem; D007814:Larva / Q000254:growth & development / Q000528:radiation effects; D000072138:Mosquito Vectors; D013696:Temperature; D014867:Water
chemical_list: D014867:Water