title: Generation of a constitutively expressing Tetracycline repressor (TetR) human embryonic stem cell line BJNhem20-TetR.
abstract: Human embryonic stem cell line BJNhem20-TetR was generated using non-viral method. The construct pCAG-TetRnls was transfected using microporation procedure. BJNhem20-TetR can subsequently be transfected with any vector harbouring a TetO (Tet operator) sequence to generate doxycycline based inducible line. For example, in human embryonic stem cells, the pSuperior based TetO system has been transfected into a TetR containing line to generate OCT4 knockdown cell line (Zafarana et al., 2009). Thus BJNhem20-TetR can be used as a tool to perturb gene expression in human embryonic stem cells.
authors.affiliation: Jawaharlal Nehru Centre for Advanced Scientific Research, Bengaluru, India.
authors.forename: Ronak
authors.initials: R
authors.lastname: Shetty
authors.affiliation: Jawaharlal Nehru Centre for Advanced Scientific Research, Bengaluru, India; Institute for Stem Cell Biology and Regenerative Medicine, Bengaluru, India.
authors.forename: Maneesha S
authors.initials: MS
authors.lastname: Inamdar
mesh_terms: D002454:Cell Differentiation; D002460:Cell Line; D058732:Embryoid Bodies / Q000166:cytology; D000066449:Human Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D059785:Karyotype; D008856:Microscopy, Fluorescence; D012333:RNA, Messenger / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D012097:Repressor Proteins; D014157:Transcription Factors