title: Use of New Techniques in Addition to IHC Applied to the Diagnosis of Melanocytic Lesions, With Emphasis on CGH, FISH, and Mass Spectrometry.
abstract: Melanoma remains one of the most aggressive forms of cutaneous malignancies. While its diagnosis based on histologic parameters is usually straight forward in most cases, distinguishing a melanoma from a melanocytic nevus can be challenging in some instances, especially when there are overlapping clinical and histopathologic features. Occasionally, melanomas can histologically mimic other tumors and even demonstration of melanocytic origin can be challenging. Thus, several ancillary tests may be employed to arrive at the correct diagnosis. The objective of this review is to summarize these tests, including the well-established and commonly used ones such as immunohistochemistry, with specific emphasis on emerging techniques such as comparative genomic hybridization, fluorescence in situ hybridization and imaging mass spectrometry.
authors.affiliation: Department of Pathology, Unit 85, University of Texas MD Anderson Cancer Center, Houston, TX 77030, United States.
authors.forename: P
authors.initials: P
authors.lastname: Nagarajan
authors.affiliation: Department of Pathology, Unit 85, University of Texas MD Anderson Cancer Center, Houston, TX 77030, United States.
authors.forename: M T
authors.initials: MT
authors.lastname: Tetzlaff
authors.affiliation: Department of Pathology, Unit 85, University of Texas MD Anderson Cancer Center, Houston, TX 77030, United States.
authors.forename: J L
authors.initials: JL
authors.lastname: Curry
authors.affiliation: Department of Pathology, Unit 85, University of Texas MD Anderson Cancer Center, Houston, TX 77030, United States. Electronic address: Vprieto@mdanderson.org.
authors.forename: V G
authors.initials: VG
authors.lastname: Prieto
mesh_terms: D000951:Antigens, Neoplasm / Q000032:analysis / Q000235:genetics; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics; D002454:Cell Differentiation; D055028:Comparative Genomic Hybridization; D003880:Dermatology / Q000379:methods*; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D013058:Mass Spectrometry; D008544:Melanocytes / Q000737:chemistry / Q000473:pathology; D008545:Melanoma / Q000737:chemistry / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D009363:Neoplasm Proteins / Q000032:analysis / Q000235:genetics; D009508:Nevus, Pigmented / Q000737:chemistry / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D011379:Prognosis; D012878:Skin Neoplasms / Q000737:chemistry / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D013194:Staining and Labeling
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins
keywords: Comparative genomic hybridization; Diagnosis; Diagnóstico; Espectometría de masas; Fluorescence in situ hybridization; Hibridación genómica comparativa; Hibridación in situ con fluorescencia; Immunohistochemistry; Inmunohistoquímica; Mass spectrometry; Melanoma