title: Flow cytometer analysis of cell apoptosis of endometrial carcinoma with Wnt10b.
abstract: The aim of this study is to analyze the cell apoptosis of endometrial carcinoma (EC) with Wnt10b by Fluorescence Activated Cell Sorting (FACS) technology. AN3CA cell lines and Ishikawa-H-12 cell lines were taken as the in-vitro cell models to observe the influence of Wnt10b on key factors of Wnt signal pathway. Methyl thiazolyl tetrazolium (MTT) was applied for the detection of cell proliferation while FACS was used for the detection of cell apoptosis. Data were analyzed using statistical software SPSS14.0. After the overexpression of Wntl0b in AN3CA cells, the apoptosis rate dropped significantly compared with the two control groups (p < 0.05); while the apoptosis rate increased significantly compared with the control groups (p < 0.01) after Wntl0b knock-off in Ishikawa3-H-12 cells. In normal endometrium, Wnt10b gene expression was negative, while that in EC cells was positive. It can be concluded that Wnt10b gene can promote EC cell proliferation and inhibit its apoptosis.
authors.affiliation: Department of Obstetrics, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Obstetrics, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
authors.forename: X L
authors.initials: XL
authors.lastname: Zhao
authors.affiliation: Department of Gynecology, the First Affiliated Hospital of Zhengzhou University, Henan, China.
authors.forename: R X
authors.initials: RX
authors.lastname: Guo
authors.affiliation: Department of Gynecology, the First Affiliated Hospital of Zhengzhou University, Henan, China.
authors.forename: Y H
authors.initials: YH
authors.lastname: Qiao
authors.affiliation: Department of Obstetrics, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
authors.forename: X Y
authors.initials: XY
authors.lastname: Zhang
authors.affiliation: Department of Gynecology, the First Affiliated Hospital of Zhengzhou University, Henan, China.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Chen
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D016889:Endometrial Neoplasms / Q000737:chemistry / Q000473:pathology*; D004717:Endometrium / Q000737:chemistry; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000235:genetics*; D051153:Wnt Proteins / Q000032:analysis / Q000235:genetics*
chemical_list: D011518:Proto-Oncogene Proteins; C491119:WNT10B protein, human; D051153:Wnt Proteins