title: Data on the characterization of follicle-stimulating hormone monoclonal antibodies and localization in Japanese eel pituitary.
abstract: Monoclonal antibodies were generated against recombinant follicle-stimulating hormone (rec-FSH) from Japanese eel Anguilla japonica; rec-FSH was produced in Escherichia coli and purified using Ni-NTA Sepharose column chromatography. In support of our recent publication, "Production and characterization of monoclonal antibodies against recombinant tethered follicle-stimulating hormone from Japanese eel Anguilla japonica" [1], it was important to characterize the specificity of eel follicle-stimulating hormone antibodies. Here, the production and ELISA system of these monoclonal antibodies are presented. The affinity-purified monoclonal antibodies specifically detected eel rec-FSH in ELISA and on western blots of rec-FSH produced from CHO cells. Immunohistochemical analysis revealed that FSH staining was specifically localized in the eel pituitary.
authors.affiliation: Aquaculture Research Division, National Institute of Fisheries Science (NIFS), Busan 46083, Republic of Korea.
authors.forename: Dae-Jung
authors.initials: DJ
authors.lastname: Kim
authors.affiliation: Animal Biotechnology, Graduate School of Future Convergence Technology, Institute of Genetic Engineering, Hankyong National University, Anseong 17579, Republic of Korea.
authors.forename: Chae-Won
authors.initials: CW
authors.lastname: Park
authors.affiliation: Animal Biotechnology, Graduate School of Future Convergence Technology, Institute of Genetic Engineering, Hankyong National University, Anseong 17579, Republic of Korea.
authors.forename: Munkhzaya
authors.initials: M
authors.lastname: Byambaragchaa
authors.affiliation: Aquaculture Research Division, National Institute of Fisheries Science (NIFS), Busan 46083, Republic of Korea.
authors.forename: Shin-Kwon
authors.initials: SK
authors.lastname: Kim
authors.affiliation: Aquaculture Research Division, National Institute of Fisheries Science (NIFS), Busan 46083, Republic of Korea.
authors.forename: Bae-Ik
authors.initials: BI
authors.lastname: Lee
authors.affiliation: Aquaculture Research Division, National Institute of Fisheries Science (NIFS), Busan 46083, Republic of Korea.
authors.forename: Hyung-Kyu
authors.initials: HK
authors.lastname: Hwang
authors.affiliation: Aquaculture Research Division, National Institute of Fisheries Science (NIFS), Busan 46083, Republic of Korea.
authors.forename: Jeong-In
authors.initials: JI
authors.lastname: Myeong
authors.affiliation: Dept. of Research and Development, Institute of Gyeongbuk Marine Bioindustry, Ulgin, 36315, Republic of Korea.
authors.forename: Sun-Mee
authors.initials: SM
authors.lastname: Hong
authors.affiliation: Department of Food Science & Nutrition, Hoseo University, Asan 31499, Republic of Korea.
authors.forename: Myung-Hwa
authors.initials: MH
authors.lastname: Kang
authors.affiliation: Animal Biotechnology, Graduate School of Future Convergence Technology, Institute of Genetic Engineering, Hankyong National University, Anseong 17579, Republic of Korea.
authors.forename: Kwan-Sik
authors.initials: KS
authors.lastname: Min
keywords: FSH; Japanese eel; Monoclonal Antibody