title: Exopolysaccharide from Trichoderma pseudokoningii promotes maturation of murine dendritic cells.
abstract: Dendritic cells (DCs) are the key regulators of immune responses. In this study, the effect of an exopolysaccharide (EPS) from the culture broth of Trichoderma pseudokoningii on the phenotypic and functional maturation of murine DCs and its underlying molecular mechanisms were investigated. It showed that EPS induced the morphological changes of DCs and the enhanced expression of DCs featured surface molecules CD11c, CD86, CD80 and major histocompatibility complex II (MHC-II). Flow cytometry analysis showed that the treatment with EPS could reduce FITC-dextran uptake by DCs. Sequentially, the results of ELISA indicated that EPS could increase the production of interleukin-12p70 (IL-12p70) in culture supernatant of DCs. Immunofluorescence staining and western blot analysis further revealed that EPS significantly prompted nuclear factor (NF)-κB subunit p65 translocation, IκB-α protein degradation, and p38 mitogen-activated protein kinase (MAPK) phosphorylation. And the production of IL-12p70 was significantly decreased in condition of the inhibition of p38 or NF-κB signaling pathway. These findings suggested that EPS could induce DCs maturation through both p38 MAPK and NF-κB signaling pathways.
authors.affiliation: Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Anhui Province Key Laboratory of Active Biological Macro-molecules, School of Pharmacy, Wannan Medical College, Wuhu 241000, China; Department of Pharmacy, Anqing Hospital Affiliated to Anhui Medical University, Anqing 246000, China.
authors.forename: Yanghui
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Anhui Province Key Laboratory of Active Biological Macro-molecules, School of Pharmacy, Wannan Medical College, Wuhu 241000, China; Department of Pharmacy, Lu'an Affiliated Hospital of Anhui Medical University, Lu'an 237000, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Pharmacy, Anqing Hospital Affiliated to Anhui Medical University, Anqing 246000, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ju
authors.affiliation: Department of Pharmacy, Lu'an Affiliated Hospital of Anhui Medical University, Lu'an 237000, China.
authors.forename: Bingxiang
authors.initials: B
authors.lastname: Shen
authors.affiliation: School of Life Science and National Glycoengineering Research Center, Shandong University, Jinan 250100, China.
authors.forename: Guochuang
authors.initials: G
authors.lastname: Chen
authors.affiliation: Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Anhui Province Key Laboratory of Active Biological Macro-molecules, School of Pharmacy, Wannan Medical College, Wuhu 241000, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Qian
authors.affiliation: Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Anhui Province Key Laboratory of Active Biological Macro-molecules, School of Pharmacy, Wannan Medical College, Wuhu 241000, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Anhui Province Key Laboratory of Active Biological Macro-molecules, School of Pharmacy, Wannan Medical College, Wuhu 241000, China.
authors.forename: Jingbo
authors.initials: J
authors.lastname: Lu
authors.affiliation: Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Anhui Province Key Laboratory of Active Biological Macro-molecules, School of Pharmacy, Wannan Medical College, Wuhu 241000, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Liu
authors.affiliation: Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Anhui Province Key Laboratory of Active Biological Macro-molecules, School of Pharmacy, Wannan Medical College, Wuhu 241000, China.
authors.forename: Guozheng
authors.initials: G
authors.lastname: Qin
authors.affiliation: Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Anhui Province Key Laboratory of Active Biological Macro-molecules, School of Pharmacy, Wannan Medical College, Wuhu 241000, China. Electronic address: guodong201@163.com.
authors.forename: Guodong
authors.initials: G
authors.lastname: Wang
authors.affiliation: Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Anhui Province Key Laboratory of Active Biological Macro-molecules, School of Pharmacy, Wannan Medical College, Wuhu 241000, China; School of Life Science and National Glycoengineering Research Center, Shandong University, Jinan 250100, China. Electronic address: ksc313@126.com.
authors.forename: Kaoshan
authors.initials: K
authors.lastname: Chen
mesh_terms: D000818:Animals; D018122:B7-1 Antigen / Q000235:genetics / Q000276:immunology; D051940:B7-2 Antigen / Q000235:genetics / Q000276:immunology; D001692:Biological Transport / Q000187:drug effects; D039521:CD11c Antigen / Q000235:genetics / Q000276:immunology; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D003911:Dextrans / Q000378:metabolism; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives / Q000378:metabolism; D062610:Fungal Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D005656:Fungal Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D005786:Gene Expression Regulation; D051550:I-kappa B Kinase / Q000235:genetics / Q000276:immunology; D018664:Interleukin-12 / Q000235:genetics / Q000276:immunology; D051379:Mice; D010766:Phosphorylation / Q000187:drug effects; D015398:Signal Transduction; D051996:Transcription Factor RelA / Q000235:genetics / Q000276:immunology; D014242:Trichoderma / Q000737:chemistry*; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics / Q000276:immunology
chemical_list: D018122:B7-1 Antigen; D051940:B7-2 Antigen; D039521:CD11c Antigen; C497965:Cd86 protein, mouse; D003911:Dextrans; D062610:Fungal Polysaccharides; D005656:Fungal Proteins; D051996:Transcription Factor RelA; C015219:fluorescein isothiocyanate dextran; D018664:Interleukin-12; C496564:Chuk protein, mouse; D051550:I-kappa B Kinase; D048051:p38 Mitogen-Activated Protein Kinases; D016650:Fluorescein-5-isothiocyanate
keywords: Exopolysaccharide; Maturation; Murine dendritic cells; Nuclear factor-κB; Trichoderma pseudokoningii; p38 MAPK