title: Protective effect of metformin on testicular ischemia/reperfusion injury in rats.
abstract: OBJECTIVE
  To investigate the protective effect of metformin on testicular ischemia/reperfusion (I/R) injury in rats.
  
  
  METHODS
  Eighteen adult male Wistar rats were randomly divided into three experimental groups (n=6), as follows: Sham, I/R, and Metformin. 1-hour ischemia was induced by the left testicular artery and vein clipping followed by 7 days of reperfusion. Metformin (100 mg/kg) was administrated orally for 7 days via oral gavage after ischemic period. At the end of trial, the left testis was removed for histological analysis and oxidative stress measurement.
  
  
  RESULTS
  I/R reduced superoxide dismutase (SOD) activities and testicular Johnsen's scores accompanied by an elevation in malondialdehyde (MDA) and myeloperoxidase (MPO) levels in comparison with the sham group (P < 0.05). Compared to I/R group, metformin restored testicular Johnsen's scores, SOD activity, MDA and MPO levels (P < 0.05).
  
  
  CONCLUSIONS
  Metformin has a protective effect against I/R injury on the testis.
authors.affiliation: Assistant Professor, Department of Clinical Science, Science and Research Branch, Islamic Azad University, Tehran, Iran. Design and supervised all phases of the study, manuscript writing.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Asghari
authors.affiliation: Assistant Professor, Department of Clinical Science, Science and Research Branch, Islamic Azad University, Tehran, Iran. Design of the study, analysis and interpretation of data.
authors.forename: Ghasem
authors.initials: G
authors.lastname: Akbari
authors.affiliation: Graduate student, Science and Research Branch, Islamic Azad University, Tehran, Iran. Technical procedures, acquisition and interpretation of data.
authors.forename: Afshin
authors.initials: A
authors.lastname: Meghdadi
authors.affiliation: Associate Professor Department of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran. Histological examinations.
authors.forename: Pejman
authors.initials: P
authors.lastname: Mortazavi
mesh_terms: D000818:Animals; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008687:Metformin / Q000494:pharmacology*; D023421:Models, Animal; D018384:Oxidative Stress / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology*; D011897:Random Allocation; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism / Q000517:prevention & control*; D013482:Superoxide Dismutase / Q000378:metabolism; D013737:Testis / Q000098:blood supply* / Q000378:metabolism
chemical_list: D020011:Protective Agents; D008315:Malondialdehyde; D008687:Metformin; D009195:Peroxidase; D013482:Superoxide Dismutase