title: Effect ofPLCεgene silencing on inhibiting the cancerous transformation of ulcerative colitis.
abstract: The aim of the present study was to investigate the effect of phosphoinositide-specific phospholipase Cε (PLCε) gene silencing on the inhibition of cancer development in ulcerative colitis (UC) and to explore the pathogenesis and carcinogenic mechanism of UC, in order to facilitate the establishment of novel strategies for the treatment of UC, prevent the cancerous transformation of UC and discern the association between inflammation and cancer. A pGenesil-PLCεRNA interference vector was constructed and transfected into HEK293 cells (pGenesil-PLCεgroup). HEK293 cells transfected with pGenesil empty plasmid were set as the negative control (pGenesil-NC group). The expression ofPLCεwas observed, and molecules associated with thePLCsignaling pathway were detected using a reverse transcription-quantitative polymerase chain reaction and western blotting. ELISA was used to determine the expression of serum interleukin-1 (IL-1) and tumor necrosis factor-α (TNF-α) of mice in which thePLCεgene had been silenced. Compared with the pGenesil-NC group, the mRNA and protein levels of PLCε were significantly decreased in the pGenesil-PLCεgroup. In addition, the mRNA levels ofK-ras,NF-κB,FasandBcl-2were markedly reduced, whileP53mRNA level was notably enhanced, in the pGenesil-PLCεgroup, and these changes were accompanied by similar changes in the corresponding protein levels. The serum IL-1 and TNF-α expression in thePLCεgene-silenced mice was significantly reduced compared with that in the control mice. In conclusion,PLCεRNA silencing can effectively inhibit the cancerous transformation of UC by regulating the colorectal cancer-related cell proliferation and cell cyclein vivo. In addition, PLCε RNA silencing can suppress the expression of inflammatory factorsin vitro.
authors.affiliation: Center of Gerontology and Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Kun
authors.initials: K
authors.lastname: Yang
authors.affiliation: Center of Gerontology and Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yan
authors.affiliation: Department of Gastroenterology, Mianyang Central Hospital, Mianyang, Sichuan 621000, P.R. China.
authors.forename: Lan
authors.initials: L
authors.lastname: Peng
authors.affiliation: Center of Gerontology and Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Yu-Pei
authors.initials: YP
authors.lastname: Zou
authors.affiliation: Center of Gerontology and Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Fu-Qian
authors.initials: FQ
authors.lastname: He
authors.affiliation: Center of Gerontology and Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Hua-Tian
authors.initials: HT
authors.lastname: Gan
authors.affiliation: Center of Gerontology and Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Huang
keywords: RNA silencing; canceration; inflammation; phosphoinositide-specific phospholipase Cε; ulcerative colitis