title: [CHROMATOSPECTROPHOTOMETRIC METHOD OF QUANTITATIVE ANALYSIS OF LAPPACONITINE IN THE UNDERGROUND PARTS OF ACONITUM ORIENTALE MILL, GROWING IN GEORGIA].
abstract: Aconitum orientale Mill (family Helleboraceae) is a perennial herb. It is spread in forests of the west and the east Georgia and in the subalpine zone. The research objects were underground parts of Aconitum orientale Mill, which were picked in the phase of fruiting in Borjomi in 2014. We had received alkaloids sum from the air-dry underground parts (1.5 kg) with chloroform extract which was alkalined by 5% sodium carbonate. We received the alkaloids sum of 16.5 g and determined that predominant is pharmacologically active diterpenic alkaloid - Lappaconitine, which is an acting initial part of the antiarrhythmic drug "Allapinin". The chromatospectrophotometrical method of quantitative analysis of Lappaconitine is elaborated for the detection of productivity of the underground parts of Aconitum orientale Mill. It was determined that maximal absorption wave length in ultra-violet spectrum (λmax) is 308 nm; It is established that relative error is norm (4%) from statical processing of quantitative analysis results. We determined that the content of Lappaconitine in the underground parts of Aconitum orientale Mill is 0.11-0.13% in the phase of fruiting. In consequence of experimental data Aconitum orientale Mill is approved as the raw material to receive pharmacologically active Lappaconitine.
authors.affiliation: Tbilisi State Medical University; I. Kutateladze Institute of Pharmacochemistry. Tbilisi, Georgia.
authors.forename: L
authors.initials: L
authors.lastname: Kintsurashvili
mesh_terms: D000157:Aconitine / Q000031:analogs & derivatives* / Q000032:analysis; D031841:Aconitum / Q000737:chemistry*; D005846:Georgia (Republic)
chemical_list: D000157:Aconitine; C022150:lappaconitine