title: Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates.
abstract: Human pluripotent stem cells (hPSCs), including embryonic stem cells and induced pluripotent stem cells, have the intrinsic ability to differentiate into all three germ layers. This makes them an attractive cell source for regenerative medicine and experimental modeling of normal and diseased organogenesis. However, the differentiation of hPSCs in vitro is heterogeneous and spatially disordered. Cell micropatterning technologies potentially offer the means to spatially control stem cell microenvironments and organize the resultant differentiation fates. Micropatterning hPSCs needs to take into account the stringent requirements for hPSC survival and maintenance. Here, we describe stencil micropatterning as a method that is highly compatible with hPSCs. hPSC micropatterns are specified by the geometries of the cell stencil through-holes, which physically confine the locations where hPSCs can access and attach to the underlying extracellular matrix-coated substrate. Due to this mode of operation, there is greater flexibility to use substrates that can adequately support hPSCs as compared to other cell micropatterning methods. We also highlight critical steps for the successful generation of hPSC micropatterns. As an example, we demonstrate that stencil micropatterning of hPSCs can be used to modulate spatial polarization of cell-cell and cell-matrix adhesions, which in turn determines mesoendoderm differentiation patterns. This simple and robust method to micropattern hPSCs widens the prospects of establishing experimental models to investigate tissue organization and patterning during early embryonic development.
authors.affiliation: Department of Biomedical Engineering, National University of Singapore.
authors.forename: Geetika
authors.initials: G
authors.lastname: Sahni
authors.affiliation: Department of Biomedical Engineering, National University of Singapore.
authors.forename: Jun
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Department of Biomedical Engineering, National University of Singapore; Singapore Institute of Neurotechnology (SINAPSE), National University of Singapore; biety@nus.edu.sg.
authors.forename: Yi-Chin
authors.initials: YC
authors.lastname: Toh
mesh_terms: D002454:Cell Differentiation; D006801:Humans; D039904:Pluripotent Stem Cells; D044968:Regenerative Medicine