title: Evaluation of oxidative response and tissular damage in rat lungs exposed to silica-coated gold nanoparticles under static magnetic fields.
abstract: The purpose of our study was the evaluation of toxicological effects of silica-coated gold nanoparticles (GNPs) and static magnetic fields (SMFs; 128 mT) exposure in rat lungs. Animals received a single injection of GNPs (1,100 µg/kg, 100 nm, intraperitoneally) and were exposed to SMFs, over 14 days (1 h/day). Results showed that GNPs treatment induced a hyperplasia of bronchus-associated lymphoid tissue. Fluorescence microscopy images showed that red fluorescence signal was detected in rat lungs after 2 weeks from the single injection of GNPs. Oxidative response study showed that GNPs exposure increased malondialdehyde level and decreased CuZn-superoxide dismutase, catalase, and glutathione peroxidase activities in rat lungs. Furthermore, the histopathological study showed that combined effects of GNPs and SMFs led to more tissular damages in rat lungs in comparison with GNPs-treated rats. Interestingly, intensity of red fluorescence signal was enhanced after exposure to SMFs indicating a higher accumulation of GNPs in rat lungs under magnetic environment. Moreover, rats coexposed to GNPs and SMFs showed an increased malondialdehyde level, a fall of CuZn-superoxide dismutase, catalase, and glutathione peroxidase activities in comparison with GNPs-treated group. Hence, SMFs exposure increased the accumulation of GNPs in rat lungs and led to more toxic effects of these nanocomplexes.
authors.affiliation: Laboratory of Integrative Physiology, Faculty Of Sciences of Bizerte, Carthage University, Carthage, Tunisia.
authors.forename: Soumaya
authors.initials: S
authors.lastname: Ferchichi
authors.affiliation: Laboratory of Integrative Physiology, Faculty Of Sciences of Bizerte, Carthage University, Carthage, Tunisia.
authors.forename: Hamdi
authors.initials: H
authors.lastname: Trabelsi
authors.affiliation: Laboratory of Integrative Physiology, Faculty Of Sciences of Bizerte, Carthage University, Carthage, Tunisia.
authors.forename: Inès
authors.initials: I
authors.lastname: Azzouz
authors.affiliation: Laboratory of Vascular Pathology, Carthage University, Carthage, Tunisia.
authors.forename: Amel
authors.initials: A
authors.lastname: Hanini
authors.affiliation: Laboratory of Pathological Anatomy, National School of Veterinary Medicine of Sidi Thabet, Manouba Univeristy, Manouba, Tunisia.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Rejeb
authors.affiliation: Laboratory of Integrative Physiology, Faculty Of Sciences of Bizerte, Carthage University, Carthage, Tunisia.
authors.forename: Olfa
authors.initials: O
authors.lastname: Tebourbi
authors.affiliation: Laboratory of Integrative Physiology, Faculty Of Sciences of Bizerte, Carthage University, Carthage, Tunisia.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Sakly
authors.affiliation: Laboratory of Integrative Physiology, Faculty Of Sciences of Bizerte, Carthage University, Carthage, Tunisia.
authors.forename: Hafedh
authors.initials: H
authors.lastname: Abdelmelek
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D005453:Fluorescence; D005979:Glutathione Peroxidase / Q000378:metabolism; D006046:Gold / Q000008:administration & dosage / Q000009:adverse effects* / Q000493:pharmacokinetics; D006965:Hyperplasia / Q000139:chemically induced; D007274:Injections, Intraperitoneal; D008168:Lung / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D060526:Magnetic Fields / Q000009:adverse effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000009:adverse effects*; D018384:Oxidative Stress / Q000187:drug effects; D017208:Rats, Wistar; D012822:Silicon Dioxide / Q000737:chemistry; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D008315:Malondialdehyde; D006046:Gold; D012822:Silicon Dioxide; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: bronchus-associated lymphoid tissue; catalase; glutathione peroxidase; histopathological study; malondialdehyde; nanotoxicity; superoxide dismutase