title: Association between the p73 gene G4C14-to-A4T14 single nucleotide polymorphism and risk of cervical cancer by high resolution melting and PCR with confronting two-pair primers in a Chinese population.
abstract: As a member of the p53 gene family, the p73 gene can affect an individual's susceptibility to cancer through a p53-like manner. DNA sequence variation in the p73 gene has been reported to be associated with cancer risk. The present study aimed to identify whether the p73 gene G4C14-to-A4T14 single nucleotide polymorphism (SNP) is associated with risk of cervical cancer in a Chinese population. The p73 G4C14-to-A4T14 polymorphism was genotyped in 175 cervical cancer and 189 healthy control peripheral blood DNA samples using high resolution melting, polymerase chain reaction with confronting two-pair primers and direct DNA sequencing. The results demonstrated that carriers of the AT/AT genotype were associated with a significantly increased risk of cervical cancer (P=0.042; χ2=4.122; odds ratio = 2.241; 95% confidence interval = 1.013-4.956) compared with the GC/GC genotype carriers. In addition, there was a significant association between p73 genotypes and tumor size in patients with cervical cancer (P=0.014; χ2=8.607). However, no association was identified between p73 genotypes and tumor stage, histological type or lymph node metastasis in patients with cervical cancer. These results suggest that the p73 G4C14-to-A4T14 SNP may function as a marker of genetic susceptibility to cervical cancer in the Chinese population.
authors.affiliation: Department of Obstetrics and Gynecology, Xi'an No. 4 Hospital, Xi'an, Shaanxi 710004, P.R. China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Guo
authors.affiliation: Institute of Biomedical Engineering, School of Geosciences and Info-Physics, Central South University, Changsha, Hunan 410000, P.R. China.
authors.forename: Shaodi
authors.initials: S
authors.lastname: Yang
authors.affiliation: Hunan Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou, Hunan 412000, P.R. China.
authors.forename: Lijian
authors.initials: L
authors.lastname: Xu
authors.affiliation: Hunan Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou, Hunan 412000, P.R. China.
authors.forename: Ding
authors.initials: D
authors.lastname: Li
authors.affiliation: Hunan Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou, Hunan 412000, P.R. China.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Tang
authors.affiliation: Hunan Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou, Hunan 412000, P.R. China.
authors.forename: Shuangshaung
authors.initials: S
authors.lastname: Wang
authors.affiliation: Yin Feng Biological Group Co., Ltd., Jinan, Shandong 250000, P.R. China.
authors.forename: Benjie
authors.initials: B
authors.lastname: Wei
authors.affiliation: Institute of Biomedical Engineering, School of Geosciences and Info-Physics, Central South University, Changsha, Hunan 410000, P.R. China.
authors.forename: Zhengchun
authors.initials: Z
authors.lastname: Liu
keywords: cervical cancer; high resolution melting; p73; polymerase chain reaction with confronting two-pair primer; polymorphism; susceptibility