title: Preparation of graphene oxide-modified affinity capillary monoliths based on three types of amino donor for chiral separation and proteolysis.
abstract: Novel graphene oxide (GO)-modified affinity capillary monoliths were developed employing human serum albumin (HSA) or pepsin as chiral selector. Three types of amino donors for GO immobilization, including ammonium hydroxide (NH4OH), ethanediamine (EDA) and polyethyleneimine (PEI), were applied to explore the effect of spacer arm on enantioseparation. It was observed that HSA-GO-EDA-based affinity capillary monoliths exhibited better chiral recognition ability in comparison with the other two spacer-based monoliths. Under the optimized conditions, the obtained columns revealed satisfactory repeatability concerning column-to-column, run-to-run and interday repeatability. In addition, the impact of GO concentration on enantiomeric separation was also investigated. HSA-GO-EDA-based affinity capillary monoliths provided higher chiral selectivity for nine pairs of enantiomers compared to the columns without GO. Furthermore, the influence of amino donors and GO on proteolytic activity of pepsin-based immobilized enzymatic reactor (IMER) was discussed. Unfortunately, pepsin-GO-PEI-based affinity capillary monoliths possessed the highest protein digestion capacity, which was different from the effect of amino donors on enantiorecognition. Moreover, GO presented as a favorable choice to improve the enzymatic activity of IMER. These results proved that GO-functionalized affinity capillary monoliths have promising potential for chiral separation and proteolysis.
authors.affiliation: Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, China; Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, Nanjing 210009, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Hong
authors.affiliation: Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, China; Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, Nanjing 210009, China.
authors.forename: Xueping
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, China; Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, Nanjing 210009, China.
authors.forename: Yujing
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, China; Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, Nanjing 210009, China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Cui
authors.affiliation: Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, China; Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, Nanjing 210009, China.
authors.forename: Ruihan
authors.initials: R
authors.lastname: Bai
authors.affiliation: Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, China; Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, Nanjing 210009, China.
authors.forename: Can
authors.initials: C
authors.lastname: Jin
authors.affiliation: Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, China; Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, Nanjing 210009, China. Electronic address: lirj04@163.com.
authors.forename: Ruijun
authors.initials: R
authors.lastname: Li
authors.affiliation: Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, China; Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, Nanjing 210009, China. Electronic address: jiyibing@msn.com.
authors.forename: Yibing
authors.initials: Y
authors.lastname: Ji
mesh_terms: D019075:Electrophoresis, Capillary; D004800:Enzymes, Immobilized / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D007202:Indicators and Reagents; D010434:Pepsin A / Q000737:chemistry; D059748:Proteolysis; D012709:Serum Albumin / Q000737:chemistry; D012822:Silicon Dioxide; D013237:Stereoisomerism
chemical_list: D004800:Enzymes, Immobilized; D007202:Indicators and Reagents; D012709:Serum Albumin; D012822:Silicon Dioxide; D006108:Graphite; D010434:Pepsin A
keywords: Capillary electrochromatography; Chiral separation; Enzyme; Graphene; Protein; Silica monolith