title: [Interferon-β combined with all-trans retinoic acid supresses proliferation and promote apoptosis by inhibiting JAK2/STAT3 pathway in HepG2 human hepatocellular carcinoma cells].
abstract: Objective To investigate the effect of interferon-β (IFN-β) combined with all-trans retinoic acid (ATRA) on the proliferation and apoptosis of HepG2 human hepatocarcinoma cells and the role of Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) signal pathway in the process. Methods HepG2 cells were randomly divided intro three groups and treated with 1000 U/mL IFN-β, 10 μmol/L ATRA and 1000 U/mL IFN-β combined with 10 μmol/L ATRA, respectively for 24 hours. Cell viability was measured by MTT assay and apoptosis rate was detected by flow cytometry. Western blotting was applied to detect the protein levels of p-JAK2, p-STAT3, gene associated with retinoid-interferon-induced mortality-19 (GRIM-19), Bcl-2, Bcl-xl and Bax. Results IFN-β or ATRA inhibited the proliferation and induced the apoptosis of HepG2 cells. The effect was enhanced when IFN-β was combined with ATRA. The expressions of p-JAK2 and p-STAT3 were down-regulated while the expressions of GRIM-19 and Bax were up-regulated after treated with IFN-β or ATRA on HepG2 cells, especially the combination of IFN-β and ATRA. Conclusion Combination of IFN-β and ATRA could suppress the proliferation and induced the apoptosis of HepG2 hepatocarcinoma cells by inhibiting JAK2/STAT3 signal pathway.
authors.affiliation: First Affiliated Hospital, Hebei Medical University, Shijiazhuang 050013, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhao
authors.affiliation: First Affiliated Hospital, Hebei Medical University, Shijiazhuang 050013, China.
authors.forename: Wenjia
authors.initials: W
authors.lastname: Nie
authors.affiliation: First Affiliated Hospital, Hebei Medical University, Shijiazhuang 050013, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: First Affiliated Hospital, Hebei Medical University, Shijiazhuang 050013, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Zhou
authors.affiliation: First Affiliated Hospital, Hebei Medical University, Shijiazhuang 050013, China.
authors.forename: Huifeng
authors.initials: H
authors.lastname: Sun
authors.affiliation: First Affiliated Hospital, Hebei Medical University, Shijiazhuang 050013, China.
authors.forename: Junqing
authors.initials: J
authors.lastname: Zhu
authors.affiliation: First Affiliated Hospital, Hebei Medical University, Shijiazhuang 050013, China.
authors.forename: Jinjun
authors.initials: J
authors.lastname: Chen
authors.affiliation: First Affiliated Hospital, Hebei Medical University, Shijiazhuang 050013, China. *Corresponding author, E-mail: 171955999@qq.com.
authors.forename: Junlu
authors.initials: J
authors.lastname: Peng
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects*; D004357:Drug Synergism; D005434:Flow Cytometry; D056945:Hep G2 Cells; D006801:Humans; D016899:Interferon-beta / Q000494:pharmacology*; D053614:Janus Kinase 2 / Q000378:metabolism*; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology; D008856:Microscopy, Fluorescence; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D014212:Tretinoin / Q000494:pharmacology*; D051028:bcl-2-Associated X Protein / Q000378:metabolism; D051020:bcl-X Protein / Q000378:metabolism
chemical_list: D051017:Apoptosis Regulatory Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D050796:STAT3 Transcription Factor; D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein; D014212:Tretinoin; D016899:Interferon-beta; D009247:NADH, NADPH Oxidoreductases; C439057:NDUFA13 protein, human; C507924:JAK2 protein, human; D053614:Janus Kinase 2