title: Large-Scale Mesenchymal Stem/Stromal Cell Expansion: A Visualization Tool for Bioprocess Comparison.
abstract: Large-scale and cost-effective cell expansion processes are a prerequisite for the clinical and commercial translation of cell-based therapies. A large variety of cell expansion processes are described in literature, utilizing different cell types, culture vessels, and medium formulations. Consequently there are no straightforward means for the comparison or benchmarking of these processes in terms of efficiency, scale, or costs. The purpose of this study was to systematically review the available mesenchymal stromal cell (MSC) expansion literature and develop an interactive visualization tool for comparing the expansion processes. By using this computational tool, process data could be concentrated, standardized, and analyzed to facilitate a more general understanding of the parameters that define a cell culture process, and in the future allow rational selection or design of these bioprocesses. Additionally, a set of bioprocess metrics were defined that assured the comparability between different processes. Currently, the literature-based data repository holds 73 individual cell expansion processes on seven different types of human MSCs in five different types of culture vessels. The visualization tool allowed benchmarking of these processes against each other, serving as a reference point for cell expansion process efficiency.
authors.affiliation: 1 M3-BIORES: Measure, Model and Manage Bioresponses , KU Leuven, Leuven, Belgium .|2 Prometheus, Division of Skeletal Tissue Engineering , KU Leuven, Leuven, Belgium.
authors.forename: Toon
authors.initials: T
authors.lastname: Lambrechts
authors.affiliation: 2 Prometheus, Division of Skeletal Tissue Engineering , KU Leuven, Leuven, Belgium.|3 Department of Metallurgy and Materials Engineering, KU Leuven, Leuven, Belgium .
authors.forename: Maarten
authors.initials: M
authors.lastname: Sonnaert
authors.affiliation: 2 Prometheus, Division of Skeletal Tissue Engineering , KU Leuven, Leuven, Belgium.|4 Antleron , Leuven, Belgium .
authors.forename: Jan
authors.initials: J
authors.lastname: Schrooten
authors.affiliation: 2 Prometheus, Division of Skeletal Tissue Engineering , KU Leuven, Leuven, Belgium.|5 Skeletal Biology and Engineering Research Center , KU Leuven, Leuven, Belgium .
authors.forename: Frank P
authors.initials: FP
authors.lastname: Luyten
authors.affiliation: 1 M3-BIORES: Measure, Model and Manage Bioresponses , KU Leuven, Leuven, Belgium .|2 Prometheus, Division of Skeletal Tissue Engineering , KU Leuven, Leuven, Belgium.
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Aerts
authors.affiliation: 2 Prometheus, Division of Skeletal Tissue Engineering , KU Leuven, Leuven, Belgium.|5 Skeletal Biology and Engineering Research Center , KU Leuven, Leuven, Belgium .
authors.forename: Ioannis
authors.initials: I
authors.lastname: Papantoniou
mesh_terms: D019149:Bioreactors; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D064987:Cell- and Tissue-Based Therapy; D006801:Humans; D059630:Mesenchymal Stem Cells
keywords: bioprocess visualization; bioreactor; cell therapy; comparability; mesenchymal stem cells; scale up