title: Chlorella virus pyrimidine dimer glycosylase and Escherichia coli endonucleases IV and V have incision activity on 2,2,4-triamino-5(2H)-oxazolone.
abstract: BACKGROUND
  2,2,4-Triamino-5(2H)-oxazolone (Oz) in a DNA strand is an oxidation product of guanine and 8-oxo-7, 8-dihydroguanine, and such a lesion can cause G-to-C transversions. Previously, Fpg/Nei and Nth were shown to have incision activity on Oz.
  
  
  RESULTS
  We investigated the activities of chlorella virus pyrimidine dimer glycosylase (cvPDG) and Escherichia coli endonucleases IV (Nfo) and V (Nfi) on Oz. Although the three enzymes have different repair mechanisms from Fpg/Nei and Nth, they still had incision activity on Oz.
  
  
  CONCLUSIONS
  Given the incision activities of cvPDG, Nfo and Nfi on Oz in addition to Fpg/Nei and Nth, Oz is DNA damage that can be repaired by diverse enzymes.
authors.affiliation: Kagawa School of Pharmaceutical Sciences, Tokushima Bunri University, 1314-1 Shido, Sanuki, Kagawa 769-2193 Japan.
authors.forename: Katsuhito
authors.initials: K
authors.lastname: Kino
authors.affiliation: Kagawa School of Pharmaceutical Sciences, Tokushima Bunri University, 1314-1 Shido, Sanuki, Kagawa 769-2193 Japan.
authors.forename: Masayo
authors.initials: M
authors.lastname: Suzuki
authors.affiliation: Kagawa School of Pharmaceutical Sciences, Tokushima Bunri University, 1314-1 Shido, Sanuki, Kagawa 769-2193 Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Morikawa
authors.affiliation: Kagawa School of Pharmaceutical Sciences, Tokushima Bunri University, 1314-1 Shido, Sanuki, Kagawa 769-2193 Japan.
authors.forename: Takanobu
authors.initials: T
authors.lastname: Kobayashi
authors.affiliation: Division of Chemistry, Graduate School of Engineering Science, Osaka University, Osaka, Japan.
authors.forename: Shigenori
authors.initials: S
authors.lastname: Iwai
authors.affiliation: Kagawa School of Pharmaceutical Sciences, Tokushima Bunri University, 1314-1 Shido, Sanuki, Kagawa 769-2193 Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Miyazawa
keywords: Chlorella virus pyrimidine dimer glycosylase; Escherichia coli endonucleases IV; Escherichia coli endonucleases V; Guanine oxidation; Oxazolone