title: Modulatory effects of caffeine on oxidative stress and anxiety-like behavior in ovariectomized rats.
abstract: Menopause is accompanied by enhanced oxidative stress and behavioral changes, effects attenuated by antioxidants. The aim of this study was to evaluate the effects of caffeine on behavior and oxidative stress in an experimental model of menopause. Female rats were divided into the following groups: sham-operated (CON), sham-operated and caffeine-treated (CAF), ovariectomized (OVX), ovariectomized and caffeine-treated (OVX+CAF). Caffeine (6 mg/kg) and vehicle were administered for 21 days (subchronic) and 42 days (chronic), using 2 experimental subsets. Behavioral tests and oxidative stress parameters in the blood, whole brain, and hippocampus were assessed. The subchronic administration of caffeine decreased the lipid peroxidation and improved the antioxidant defense in the blood and brain. The GSH/GGSG ratio in the brain was improved by chronic administration, with reduced activities of antioxidant enzymes and enhanced nitric oxide and malondialdehyde levels. In particular, the lipid peroxidation in the hippocampus decreased in both experiments. The rats became hyperactive after 21 days of treatment, but no effect was observed after chronic administration. In both experimental subsets, caffeine had anxiolytic effects as tested in elevated plus maze. The administration of low doses of caffeine, for a short period of time, may be a new therapeutic approach to modulating the oxidative stress and anxiety in menopause.
authors.affiliation: Department of Physiology, "Iuliu Hatieganu" University of Medicine and Pharmacy, 1 Clinicilor Street, 400006 Cluj-Napoca, Romania.|Department of Physiology, "Iuliu Hatieganu" University of Medicine and Pharmacy, 1 Clinicilor Street, 400006 Cluj-Napoca, Romania.
authors.forename: Ionut
authors.initials: I
authors.lastname: Caravan
authors.affiliation: Department of Physiology, "Iuliu Hatieganu" University of Medicine and Pharmacy, 1 Clinicilor Street, 400006 Cluj-Napoca, Romania.|Department of Physiology, "Iuliu Hatieganu" University of Medicine and Pharmacy, 1 Clinicilor Street, 400006 Cluj-Napoca, Romania.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Sevastre Berghian
authors.affiliation: Department of Physiology, "Iuliu Hatieganu" University of Medicine and Pharmacy, 1 Clinicilor Street, 400006 Cluj-Napoca, Romania.|Department of Physiology, "Iuliu Hatieganu" University of Medicine and Pharmacy, 1 Clinicilor Street, 400006 Cluj-Napoca, Romania.
authors.forename: Remus
authors.initials: R
authors.lastname: Moldovan
authors.affiliation: Department of Physiology, "Iuliu Hatieganu" University of Medicine and Pharmacy, 1 Clinicilor Street, 400006 Cluj-Napoca, Romania.|Department of Physiology, "Iuliu Hatieganu" University of Medicine and Pharmacy, 1 Clinicilor Street, 400006 Cluj-Napoca, Romania.
authors.forename: Nicoleta
authors.initials: N
authors.lastname: Decea
authors.affiliation: Department of Physiology, "Iuliu Hatieganu" University of Medicine and Pharmacy, 1 Clinicilor Street, 400006 Cluj-Napoca, Romania.|Department of Physiology, "Iuliu Hatieganu" University of Medicine and Pharmacy, 1 Clinicilor Street, 400006 Cluj-Napoca, Romania.
authors.forename: Remus
authors.initials: R
authors.lastname: Orasan
authors.affiliation: Department of Physiology, "Iuliu Hatieganu" University of Medicine and Pharmacy, 1 Clinicilor Street, 400006 Cluj-Napoca, Romania.|Department of Physiology, "Iuliu Hatieganu" University of Medicine and Pharmacy, 1 Clinicilor Street, 400006 Cluj-Napoca, Romania.
authors.forename: Gabriela Adriana
authors.initials: GA
authors.lastname: Filip
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001007:Anxiety / Q000378:metabolism*; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002110:Caffeine / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D005260:Female; D005978:Glutathione / Q000097:blood / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000097:blood / Q000378:metabolism; D018782:Maze Learning / Q000187:drug effects*; D008593:Menopause / Q000187:drug effects; D009569:Nitric Oxide / Q000378:metabolism; D010052:Ovariectomy; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats
chemical_list: D000975:Antioxidants; D009569:Nitric Oxide; D002110:Caffeine; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D005978:Glutathione
keywords: behavior; blood; brain; caffeine; caféine; cerveau; comportement; neuroprotection; ovariectomie; ovariectomy; oxidative stress; rats; sang; stress oxydatif