title: Association of Interleukin-10 (A1082G) gene polymorphism with Oral squamous cell carcinoma in north Indian population.
abstract: The functional polymorphism A1082G in the gene (IL10) for interleukin-10 associated with risk of oral squamous cell carcinoma (OSCC). The present case-control study was to evaluate the possible association between IL10 A1082G gene and OSCC in north Indian population. Analysis of IL10 A1082G genotype in 232 OSCC cases and 221 healthy controls of comparable age, gender, smokers, tobacco chewing and alcohol consumption. IL10 A1082G status in cases and controls were evaluated by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). The frequencies of IL10 A1082G polymorphism AA, AG, GG genotypes were 29.74, 68.10 and 2.15% in OSCC cases and 57.46, 42.08 and 0.45% in healthy controls. The average frequency of G mutant allele was 36.20% in OSCC cases compared with 21.50% among the controls and this allele was associated with increased risk for OSCC cases. Heterozygous AG genotype was found statistically significant in OSCC cases than in controls (OR = 1.6, 95% CI = 1.1-2.2, P = 0.003), whereas homozygous mutant GG genotype was not found significant (OR = 4.7, 95% CI = 0.55-41.1, P = 0.2). Moreover, we found that G allele was significant in OSCC cases of tobacco chewing. The frequency of IL10 A1082G polymorphism G allele and AG genotype is associated with OSCC cases as compared with controls; this may be due to smoking and tobacco chewing. Our findings showed that in IL10 A1082G gene polymorphism AG genotype and G allele may participate in the progression of OSCC.
authors.affiliation: Molecular Cell Biology Lab, Department of Biochemistry,King George's Medical University, Lucknow 226 003, India.sgmds2002@yahoo.co.in.
authors.forename: Syed Rizwan
authors.initials: SR
authors.lastname: Hussain
authors.forename: Mohammad Kaleem
authors.initials: MK
authors.lastname: Ahmad
authors.forename: Abbas Ali
authors.initials: AA
authors.lastname: Mahdi
authors.forename: Hena
authors.initials: H
authors.lastname: Naqvi
authors.forename: Mohammad Waseem
authors.initials: MW
authors.lastname: Ahmad
authors.forename: Saurabh
authors.initials: S
authors.lastname: Srivastava
authors.forename: Kumud
authors.initials: K
authors.lastname: Nigam
authors.forename: Shalini
authors.initials: S
authors.lastname: Gupta
mesh_terms: D000328:Adult; D000483:Alleles; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000209:etiology / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D015870:Gene Expression; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D007194:India; D016753:Interleukin-10 / Q000235:genetics*; D008297:Male; D009062:Mouth Neoplasms / Q000175:diagnosis / Q000209:etiology / Q000235:genetics* / Q000473:pathology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects; D014030:Tobacco, Smokeless / Q000009:adverse effects
chemical_list: C508609:IL10 protein, human; D016753:Interleukin-10