title: Acute stress induces increases in salivary IL-10 levels.
abstract: The purpose of this study was to investigate the stress-reactivity of the anti-inflammatory cytokine, IL-10, in saliva and to determine how salivary IL-10 levels change in relation to those of IL-1β, a pro-inflammatory cytokine, following stress. Healthy young adults were randomly assigned to retrieve a negative emotional memory (n = 46) or complete a modified version of the Trier Social Stress Test (n = 45). Saliva samples were taken 10 min before (baseline) and 50 min after (post-stressor) onset of a 10-min stressor, and were assayed using a high sensitivity multiplex assay for cytokines. Measurable IL-10 levels (above the minimum detectable concentration) were found in 96% of the baseline samples, and 98% of the post-stressor samples. Flow rate-adjusted salivary IL-10 levels as well as IL-1β/IL-10 ratios showed moderate but statistically significant increases in response to stress. Measurement of salivary IL-10 and pro-/anti-inflammatory cytokine ratios may be useful, noninvasive tools, in stress research.
authors.affiliation: a Department of Psychological and Brain Sciences , University of Louisville , Louisville , KY , USA ;
authors.forename: Yvette Z
authors.initials: YZ
authors.lastname: Szabo
authors.affiliation: a Department of Psychological and Brain Sciences , University of Louisville , Louisville , KY , USA ;
authors.forename: Tamara L
authors.initials: TL
authors.lastname: Newton
authors.affiliation: b Department of Pathology and Laboratory Medicine , University of Louisville , Louisville , KY , USA.
authors.forename: James J
authors.initials: JJ
authors.lastname: Miller
authors.affiliation: a Department of Psychological and Brain Sciences , University of Louisville , Louisville , KY , USA ;
authors.forename: Keith B
authors.initials: KB
authors.lastname: Lyle
authors.affiliation: b Department of Pathology and Laboratory Medicine , University of Louisville , Louisville , KY , USA.
authors.forename: Rafael
authors.initials: R
authors.lastname: Fernandez-Botran
mesh_terms: D000293:Adolescent; D000328:Adult; D004644:Emotions / Q000502:physiology*; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000032:analysis*; D053583:Interleukin-1beta / Q000032:analysis; D008297:Male; D008568:Memory / Q000502:physiology; D012463:Saliva / Q000737:chemistry*; D013315:Stress, Psychological / Q000503:physiopathology* / Q000523:psychology; D055815:Young Adult
chemical_list: C508609:IL10 protein, human; D053583:Interleukin-1beta; D016753:Interleukin-10
keywords: IL-1β/IL-10 ratio; Interleukin-10; TSST; anti-inflammatory; emotional memory; saliva