title: [Determination of Nucleosides and Nucleobases in Natural, Cultured and Tissue Culture Anoectochilus roxburghii Using LC-MS].
abstract: OBJECTIVE
  To establish a method for simultaneous determination of nucleosides and nucleobases in natural, cultured and tissue culture Anoectochilus roxburghii by high performance liquid chromatography-electrospray ionization/ion trap mass spectrometry (HPLC-ESI/MS).
  
  
  METHODS
  The separation was performed on a Welch Ultimate XB-C18 column (250 mm x 4.6 mm,5 μm). 20 mmol/L ammonium acetate solution and methanol were adopted as the mobile phase with gradient elution. The flow rate was 1.0 mL/min. The injection volume was 20 μL. The column temperature and UV wavelength were set at 30 degrees C and 260 nm, respectively.
  
  
  RESULTS
  Cytosine, uracil, cytidine, uridine, hypoxanthine, adenine, inosine, guanosine,fl-thymidine and adenosine were identified in natural, cultured and tissue culture Anoectochilus roxburghii. The total content of nucleosides and nucleotides in Anoectochilus roxburghii were 1.6639, 1.8568 and 2.2013 mg/g,respectively.
  
  
  CONCLUSIONS
  The contents of nucleosides and nucleobases in herb are affected by its growth pattern. The total content of nucleosides and nucleotides was tissue culture herb > cultured herb > natural herb. This investigation would provide the theoretic basis for quality standards and applications of Anoectochilus roxburghii in clinical research.
authors.forename: Li-hong
authors.initials: LH
authors.lastname: Zheng
authors.forename: Li-ying
authors.initials: LY
authors.lastname: Huang
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.forename: Shou-er
authors.initials: SE
authors.lastname: Lin
authors.forename: Bing-lan
authors.initials: BL
authors.lastname: Huang
mesh_terms: D000225:Adenine / Q000032:analysis; D000241:Adenosine / Q000032:analysis; D002853:Chromatography, Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D006151:Guanosine / Q000032:analysis; D019271:Hypoxanthine / Q000032:analysis; D013058:Mass Spectrometry; D009705:Nucleosides / Q000032:analysis*; D009711:Nucleotides / Q000032:analysis*; D029595:Orchidaceae / Q000737:chemistry*; D014498:Uracil / Q000032:analysis; D014529:Uridine / Q000032:analysis
chemical_list: D004365:Drugs, Chinese Herbal; D009705:Nucleosides; D009711:Nucleotides; D006151:Guanosine; D019271:Hypoxanthine; D014498:Uracil; D000225:Adenine; D000241:Adenosine; D014529:Uridine