title: [Expression of microRNA-29b in colorectal cancer and its relationship with clinicopathological features].
abstract: OBJECTIVE
  The aim of this study was to detect the expression level of miR-29b in colorectal cancer (CRC) tissues and to analyze its relationship with clinicopathological features and prognosis.
  
  
  METHODS
  The expression of miR-29b was detected by real-time quantitative PCR in 202 colorectal cancer tissues and adjacent colorectal tissues. Statistical analysis of the results was conducted using SPSS 16.0 software.
  
  
  RESULTS
  The expression of miR-29b in colorectal cancer tissues and adjacent colorectal tissues was -7.761±0.115 and -7.150±0.137, respectively, with a significant difference between the two groups (P<0.01). Comparing colorectal cancer tissues with lymph node metastasis with those without lymph node metastasis, the expression of miR-29b (-7.528±0.158 vs. -7.988±0.164) was significantly increased (P<0.05). In addition, the expression of miR-29b in CRC according to TNM stage was -8.096±0.157 in stage Ⅰ/Ⅱ, -7.592±0.165 in stage Ⅲ and -6.603±0.468 in stage Ⅳ patients, showing a gradual increase depending on clinical staging (P<0.05). Univariate Cox regression analysis indicated that lymph node metastasis, differentiation degree and TNM stage were significantly related to the postoperative survival of colorectal cancer patients (P<0.001). Multivariate Cox regression analysis indicated that differentiation degree and TNM stage were independent factors impacting the survival of colorectal cancer patients (P<0.001).
  
  
  CONCLUSIONS
  The expression level of miR-29b is reduced in colorectal cancer tissues compared with that in the adjacent colorectal tissues. High expression level of miR-29b is associated with lymph node metastasis and TNM stage of CRC. miR-29b may be a potential marker indicating colorectal cancer metastasis and malignant progression.
authors.affiliation: State Key Laboratory of Molecular Oncology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
authors.forename: X
authors.initials: X
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Molecular Oncology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
authors.forename: W
authors.initials: W
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Molecular Oncology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
authors.forename: W Y
authors.initials: WY
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Molecular Oncology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.
authors.forename: J
authors.initials: J
authors.lastname: Ma
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism; D002454:Cell Differentiation; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology; D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D035683:MicroRNAs / Q000378:metabolism*; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; C532045:MIRN29a microRNA, human; D035683:MicroRNAs