title: Circulation Enrichment of Functional Endothelial Progenitor Cells by Infantile Phototherapy.
abstract: Phototherapy is the most common therapy used for severe jaundice. There is increasing evidence that phototherapy can directly affect the expression and function of cell surface receptors including adhesion molecules, cytokines, and growth factor receptors. In this study, the effect of two infantile phototherapy regimens, including single and intensive phototherapy was investigated on biological features of circulation endothelial progenitor cells (cEPCs), as well as on serum secretion of two important chemotactic cytokines, SDF-1 and VEGF. Sixty infants diagnosed with severe hyperbilirubinemia and exposed to phototherapy were enrolled in this study. cEPCs were isolated before and after phototherapy and then migratory, proliferative, tubulogenic, and functional properties of these cells were analyzed. Our results revealed that intensive phototherapy markedly increased the release of EPCs into the circulation, and augmented the serum concentrations of both SDF-1 and VEGF cytokines. Cell proliferation, tubulogenic, and migratory properties of cEPCs isolated and expanded from infants with intensive phototherapy were significantly improved. cEPCs from infants with intensive phototherapy also showed greater levels of acetylated low-density lipoprotein and lectin binding. Overall, our results showed that the intensive phototherapy regimen can mobilize functional EPCs into the circulation through up-regulation of serum levels of VEGF and SDF-1, indicating phototherapy as an effective modality for improvement of stem cell mobilization in the therapeutic regenerative medicine. J. Cell. Biochem. 118: 330-340, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors.forename: Vahid
authors.initials: V
authors.lastname: Siavashi
authors.affiliation: Imam Reza Hospital, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors.forename: Simin
authors.initials: S
authors.lastname: Asadian
authors.affiliation: Faculty Member, School of Nahavand Paramedical, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Azam
authors.initials: A
authors.lastname: Sharifi
authors.affiliation: School of Nursing and Midwifery, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors.forename: Masoumeh
authors.initials: M
authors.lastname: Esmaeilivand
authors.affiliation: Faculty Member, School of Paramedical Sciences, ALborz University of Medical Sciences, Karaj, Iran.
authors.forename: Roohangiz
authors.initials: R
authors.lastname: Norouzinia
authors.affiliation: Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Azadbakht
authors.affiliation: Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors.forename: Seyed Mahdi
authors.initials: SM
authors.lastname: Nassiri
mesh_terms: D054377:Chemokine CXCL12 / Q000097:blood*; D066026:Endothelial Progenitor Cells / Q000378:metabolism*; D005260:Female; D006801:Humans; D006932:Hyperbilirubinemia / Q000097:blood / Q000628:therapy; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D010789:Phototherapy; D042461:Vascular Endothelial Growth Factor A / Q000097:blood*
chemical_list: C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A
keywords: CELL PROLIFERATION; ENDOTHELIAL PROGENITOR CELL; MIGRATION; PHOTOTHERAPY