title: Identification and characterization of a pathogenicity-related gene VdCYP1 from Verticillium dahliae.
abstract: Verticillium dahliae is a phytopathogenic fungus that causes vascular wilt disease in a wide variety of crop plants, thereby causing extensive economic loss. In present study, one V. dahliae T-DNA mutant M01C06 showed the pathogenicity loss on cotton, and the expression of a flanking gene encoding cytochrome P450 monooxygenase (P450, VdCYP1) was strongly repressed. P450s of fungi could affect the fungal pathogenicity by involving in the synthesis of secondary metabolites. However, there was no report about the pathogenic function of P450s in V. dahliae. VdCYP1 gene deletion and complementation experiments confirmed that VdCYP1 was the pathogenicity-related gene in V. dahliae. A comparison of culture supernatants of the VdCYP1 deletion mutants and wild-type strains indicates that at least 14 kinds of secondary metabolites syntheses were affected due to VdCYP1 gene deletion. One of these compounds, sulfacetamide, had the ability to induce the necrosis and wilting symptoms in cotton. Above results indicate that VdCYP1 could participate in pathogenesis by involving the secondary metabolism in V. dahliae, such as the compound sulfacetamide. In conclusion, VdCYP1 acts as an important pathogenicity-related factor to involve in secondary metabolism that likely contributes to the pathogenic process in V. dahliae.
authors.affiliation: The Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China.
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Zhang
authors.affiliation: The Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China.
authors.forename: Xin-Yan
authors.initials: XY
authors.lastname: Wang
authors.affiliation: The Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China.
authors.forename: Jie-Yin
authors.initials: JY
authors.lastname: Chen
authors.affiliation: The Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China.
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Kong
authors.affiliation: The Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China.
authors.forename: Yue-Jing
authors.initials: YJ
authors.lastname: Gui
authors.affiliation: The Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China.
authors.forename: Nan-Yang
authors.initials: NY
authors.lastname: Li
authors.affiliation: The Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China.
authors.forename: Yu-Ming
authors.initials: YM
authors.lastname: Bao
authors.affiliation: The Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China.
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Dai
mesh_terms: D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D003368:Gossypium / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology; D013172:Spores, Fungal / Q000235:genetics; D013409:Sulfacetamide / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics*; D020172:Verticillium / Q000235:genetics* / Q000472:pathogenicity*; D014774:Virulence
chemical_list: D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; C036483:T-DNA; D014157:Transcription Factors; D013409:Sulfacetamide; D003577:Cytochrome P-450 Enzyme System