title: [Application of Cytogenetic Test for Diagnosis of Bone Marrow Involvement in Patients with Non-Hodgkin's Lymphoma].
abstract: OBJECTIVE
  To investigate the role of cytogenetic analysis in the detection of bone marrow (BM) involvement in patients with non-Hodgkin's lymphoma (NHL).
  
  
  METHODS
  The bone marrow samples of 74 patients with NHL were detection by using morphology, cytogenetic test, flow cytometry and molecular biological assay. The detected results of morphology, cytogenetic test, flow cytometry and molecular biological assay alone and thier combined detection were compared, the detective rate and consistencies of the 4 methods were analyzed.
  
  
  RESULTS
  The detection rates of BM involvement by using morphology, cytogenetic, flow cytometry, and molecular biological assays were 21.6%, 17.6%, 23.0% and 33.8% respectively. The detective rate was enhanced to 44.6% by combining the 4 methods. Cytogenetic test showed the result consistent with the other methods.
  
  
  CONCLUSIONS
  Although cytogenetic test shows a lower detective rate than the other methods, but in some patients the cytogenetic test can detect the abnormality of bone marrow which can not be detected by other methods alone, the combination test of 4 detection methods can enhance the detectable rate of BM involvement.
authors.affiliation: Department of Hematology, China-Japan Friendship Hospital, Beijing 100029, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Department of Hematology, China-Japan Friendship Hospital, Beijing 100029, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Hematology, China-Japan Friendship Hospital, Beijing 100029, China.
authors.forename: Li
authors.initials: L
authors.lastname: Gao
authors.affiliation: Department of Hematology, China-Japan Friendship Hospital, Beijing 100029, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Hematology, China-Japan Friendship Hospital, Beijing 100029, China.
authors.forename: Ya-Yue
authors.initials: YY
authors.lastname: Gao
authors.affiliation: Department of Hematology, China-Japan Friendship Hospital, Beijing 100029, China.
authors.forename: Chun-Xia
authors.initials: CX
authors.lastname: Zhang
authors.affiliation: Department of Hematology, China-Japan Friendship Hospital, Beijing 100029, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Gong
authors.affiliation: Department of Hematology, China-Japan Friendship Hospital, Beijing 100029, China.
authors.forename: Fan-Zhou
authors.initials: FZ
authors.lastname: Huang
authors.affiliation: Department of Hematology, China-Japan Friendship Hospital, Beijing 100029, China.
authors.forename: Zhen-Ling
authors.initials: ZL
authors.lastname: Li
authors.affiliation: Department of Hematology, China-Japan Friendship Hospital, Beijing 100029, China. E-mail: dr_myg@163.com.
authors.forename: Yi-Gai
authors.initials: YG
authors.lastname: Ma
mesh_terms: D001853:Bone Marrow / Q000473:pathology*; D001856:Bone Marrow Examination; D020732:Cytogenetic Analysis; D005434:Flow Cytometry; D006801:Humans; D008228:Lymphoma, Non-Hodgkin / Q000175:diagnosis* / Q000235:genetics