title: Characterization of the Rat GAL2R Promoter: Positive Role of ETS-1 in Regulation of the Rat GAL2R Gene in PC12 Cells.
abstract: Galanin receptor 2 (GAL2R) is a G protein-coupled receptor for the neuropeptide galanin that regulates many important physiological functions and pathological processes. To investigate the molecular mechanism governing GAL2R gene transcription, the rat GAL2R promoter was isolated and analyzed. We found that the region from -320 to -300 of the GAL2R promoter contains two putative ETS-1 elements and plays an important role in regulating GAL2R promoter activity. We also showed that transcription factor ETS-1 bound to this region in vitro and in vivo. Overexpression of ETS-1 significantly increased GAL2R promoter activity and transcription of the GAL2R gene, whereas knockdown of ETS-1 produced the opposite effects. In addition, we showed that ETS-1 recruited co-activator p300 to the GAL2R promoter. These data indicate a role for ETS-1 in the control of the GAL2R gene expression and provide a basis for understanding the transcriptional regulation of the GAL2R gene.
authors.affiliation: Department of Neurobiology, Beijing Center of Neural Regeneration and Repair, Beijing Key Laboratory of Brain Disorders (MOST), Beijing Institute for Brain Disorders, Capital Medical University, Beijing, 100069, China.
authors.forename: Yutao
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Neurobiology, Beijing Center of Neural Regeneration and Repair, Beijing Key Laboratory of Brain Disorders (MOST), Beijing Institute for Brain Disorders, Capital Medical University, Beijing, 100069, China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Neurobiology, Beijing Center of Neural Regeneration and Repair, Beijing Key Laboratory of Brain Disorders (MOST), Beijing Institute for Brain Disorders, Capital Medical University, Beijing, 100069, China.
authors.forename: Hanjiang
authors.initials: H
authors.lastname: Luo
authors.affiliation: Department of Neurobiology, Beijing Center of Neural Regeneration and Repair, Beijing Key Laboratory of Brain Disorders (MOST), Beijing Institute for Brain Disorders, Capital Medical University, Beijing, 100069, China.
authors.forename: Yueting
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of anatomy and histology, Capital Medical University, Beijing, 100069, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Neurobiology, Beijing Center of Neural Regeneration and Repair, Beijing Key Laboratory of Brain Disorders (MOST), Beijing Institute for Brain Disorders, Capital Medical University, Beijing, 100069, China. zhiqingx@ccmu.edu.cn.
authors.forename: Zhi-Qing David
authors.initials: ZD
authors.lastname: Xu
mesh_terms: D000818:Animals; D001483:Base Sequence; D050881:E1A-Associated p300 Protein; D005786:Gene Expression Regulation; D016716:PC12 Cells; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D050784:Proto-Oncogene Protein c-ets-1 / Q000378:metabolism*; D051381:Rats; D044092:Receptor, Galanin, Type 2 / Q000235:genetics* / Q000378:metabolism; D024363:Transcription Initiation Site
chemical_list: D050784:Proto-Oncogene Protein c-ets-1; D044092:Receptor, Galanin, Type 2; D050881:E1A-Associated p300 Protein
keywords: ETS-1; GAL2R; Promoter; Transcriptional regulation; p300