title: The Development of a Novel, Validated, Rapid and Simple Method for the Detection of Sarcocystis fayeri in Horse Meat in the Sanitary Control Setting.
abstract: Sarcocystis fayeri (S. fayeri) is a newly identified causative agent of foodborne disease that is associated with the consumption of raw horse meat. The testing methods prescribed by the Ministry of Health, Labour and Welfare of Japan are time consuming and require the use of expensive equipment and a high level of technical expertise. Accordingly, these methods are not suitable for use in the routine sanitary control setting to prevent outbreaks of foodborne disease. In order to solve these problems, we have developed a new, rapid and simple testing method using LAMP, which takes only 1 hour to perform and which does not involve the use of any expensive equipment or expert techniques. For the validation of this method, an inter-laboratory study was performed among 5 institutes using 10 samples infected with various concentrations of S. fayeri. The results of the inter-laboratory study demonstrated that our LAMP method could detect S. fayeri at concentrations greater than 10(4) copies/g. Thus, this new method could be useful in screening for S. fayeri as a routine sanitary control procedure.
authors.affiliation: Kumamoto Prefectual Institute of Public-Health and Environmental Science.
authors.forename: Masato
authors.initials: M
authors.lastname: Furukawa
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Minegishi
authors.forename: Shinji
authors.initials: S
authors.lastname: Izumiyama
authors.forename: Kenji
authors.initials: K
authors.lastname: Yagita
authors.forename: Hideto
authors.initials: H
authors.lastname: Mori
authors.forename: Taku
authors.initials: T
authors.lastname: Uemura
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Etoh
authors.forename: Eriko
authors.initials: E
authors.lastname: Maeda
authors.forename: Mari
authors.initials: M
authors.lastname: Sasaki
authors.forename: Kazuya
authors.initials: K
authors.lastname: Ichinose
authors.forename: Seiya
authors.initials: S
authors.lastname: Harada
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Kamata
authors.forename: Masaki
authors.initials: M
authors.lastname: Otagiri
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Sugita-Konishi
authors.forename: Takahiro
authors.initials: T
authors.lastname: Ohnishi
mesh_terms: D000818:Animals; D005504:Food Analysis; D059022:Food Safety; D005517:Foodborne Diseases / Q000469:parasitology / Q000517:prevention & control; D006736:Horses; D006801:Humans; D008460:Meat / Q000469:parasitology*; D021141:Nucleic Acid Amplification Techniques; D015203:Reproducibility of Results; D012522:Sarcocystis / Q000145:classification* / Q000235:genetics*