title: Toxicological Evaluation of Low Molecular Weight Fucoidan in Vitro and in Vivo.
abstract: For a long time, fucoidan has been well known for its pharmacological activities, and recently low molecular weight fucoidan (LMF) has been used in food supplements and pharmaceutical products. In the present study, LMF was extracted from Laminaria japonica by enzyme hydrolysis. The toxicity of LMF in mouse and rat models was determined by many methods, such as total arsenic content, bacterial reverse mutation assay, chromosome aberration assay, and in vivo micronucleus assay. The present findings showed that LMF at 5000 μg/mL exhibited no mutagenicity. It also produced no formatting disruption of red blood cells in vivo. At 2000 mg/kg BW/day there were no toxicological indications. LMF is expected to be used as a safe food supplement.
authors.affiliation: Department of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung 20246, Taiwan. amperehwang@gmail.com.
authors.forename: Pai-An
authors.initials: PA
authors.lastname: Hwang
authors.affiliation: Cancer Center, Wan Fang Hospital, Taipei Medical University, Taipei 11696, Taiwan. yanmd717@gmail.com.
authors.forename: Ming-De
authors.initials: MD
authors.lastname: Yan
authors.affiliation: Department of Food Science, National Taiwan Ocean University, Keelung 20246, Taiwan. HL358@ntou.edu.tw.
authors.forename: Hong-Ting Victor
authors.initials: HT
authors.lastname: Lin
authors.affiliation: Graduate Institute of Biotechnology, Chinese Culture University, Taipei 11114, Taiwan. noodleplusalan@gmail.com.
authors.forename: Kuan-Lun
authors.initials: KL
authors.lastname: Li
authors.affiliation: Graduate Institute of Biotechnology, Chinese Culture University, Taipei 11114, Taiwan. lyc10@ulive.pccu.edu.tw.
authors.forename: Yen-Chang
authors.initials: YC
authors.lastname: Lin
mesh_terms: D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D003412:Cricetulus; D019587:Dietary Supplements / Q000009:adverse effects; D005260:Female; D007795:Laminaria / Q000737:chemistry; D008297:Male; D051379:Mice; D008970:Molecular Weight; D011134:Polysaccharides / Q000009:adverse effects*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D011134:Polysaccharides; C007789:fucoidan
keywords: Laminaria japonica; low molecular weight fucoidan; toxicity