title: Techniques for Screening Translation Inhibitors.
abstract: The machinery of translation is one of the most common targets of antibiotics. The development and screening of new antibiotics usually proceeds by testing antimicrobial activity followed by laborious studies of the mechanism of action. High-throughput methods for new antibiotic screening based on antimicrobial activity have become routine; however, identification of molecular targets is usually a challenge. Therefore, it is highly beneficial to combine primary screening with the identification of the mechanism of action. In this review, we describe a collection of methods for screening translation inhibitors, with a special emphasis on methods which can be performed in a high-throughput manner.
authors.affiliation: Department of Chemistry and A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia. osterman@yandex.ru.
authors.forename: Ilya A
authors.initials: IA
authors.lastname: Osterman
authors.affiliation: Department of Chemistry and A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia. bogdanov@genebee.msu.ru.
authors.forename: Alexey A
authors.initials: AA
authors.lastname: Bogdanov
authors.affiliation: Department of Chemistry and A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia. olga.a.dontsova@gmail.com.
authors.forename: Olga A
authors.initials: OA
authors.lastname: Dontsova
authors.affiliation: Department of Chemistry and A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia. petya@genebee.msu.ru.
authors.forename: Petr V
authors.initials: PV
authors.lastname: Sergiev
keywords: antibiotic; high throughput screening; ribosome; translation