title: Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition.
abstract: Fibrosis is often involved in the pathogenesis of various chronic progressive diseases such as interstitial pulmonary disease. Pathological hallmark is the formation of fibroblastic foci, which is associated with the disease severity. Mesenchymal cells consisting of the fibroblastic foci are proposed to be derived from several cell sources, including originally resident intrapulmonary fibroblasts and circulating fibrocytes from bone marrow. Recently, mesenchymal cells that underwent epithelial-mesenchymal transition (EMT) have been also supposed to contribute to the pathogenesis of fibrosis. In addition, EMT can be induced by transforming growth factor β, and EMT can be enhanced by pro-inflammatory cytokines like tumor necrosis factor α. The gel contraction assay is an ideal in vitro model for the evaluation of contractility, which is one of the characteristic functions of fibroblasts and contributes to wound repair and fibrosis. Here, the development of a gel contraction assay is demonstrated for evaluating contractile ability of mesenchymal cells that underwent EMT.
authors.affiliation: Department of Clinical Laboratory, The University of Tokyo Hospital; Department of Respiratory Medicine, The University of Tokyo Hospital.
authors.forename: Yu
authors.initials: Y
authors.lastname: Mikami
authors.affiliation: Department of Respiratory Medicine, The University of Tokyo Hospital.
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Matsuzaki
authors.affiliation: Department of Respiratory Medicine, The University of Tokyo Hospital.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Takeshima
authors.affiliation: Department of Respiratory Medicine, The University of Tokyo Hospital.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Makita
authors.affiliation: Department of Respiratory Medicine, The University of Tokyo Hospital; YAMAUCHIY-INT@h.u-tokyo.ac.jp.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Yamauchi
authors.affiliation: Department of Respiratory Medicine, The University of Tokyo Hospital.
authors.forename: Takahide
authors.initials: T
authors.lastname: Nagase
mesh_terms: D058750:Epithelial-Mesenchymal Transition; D005347:Fibroblasts; D005355:Fibrosis; D059630:Mesenchymal Stem Cells; D016212:Transforming Growth Factor beta
chemical_list: D016212:Transforming Growth Factor beta