title: Antidiabetic Mechanisms of Rosa canina Fruits: An In Vitro Evaluation.
abstract: Rosa canina fruits have been used traditionally for the treatment of diabetes mellitus and its complications. The aim of current study was to evaluate the in vitro mechanism of action of R canina in managing diabetes mellitus. Cell proliferation and cytotoxicity assay were performed on pancreatic β-cells, βTC6. The protective activity of the extract on streptozotocin-induced death in βTC6 cells was studied. The effect of R canina on the metabolism of glucose in HepG2, a hepatocellular carcinoma cell line, was evaluated. The effect of the extract on glucose diffusion across the dialysis membrane, which is a comfortable model for assessing cellular glucose absorption, was evaluated. The results obtained from current study confirmed that R canina extract can act as a growth factor for pancreatic β-cell line providing a novel mechanism for the observed antidiabetic effect of this natural agent. Further preclinical studies are necessary to evaluate the perfect mechanism of action of R canina in diabetes mellitus.
authors.affiliation: Pharmaceutical Sciences Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.|Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Fattahi
authors.affiliation: Students Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Niyazi
authors.affiliation: Pharmaceutical Sciences Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors.forename: Behzad
authors.initials: B
authors.lastname: Shahbazi
authors.affiliation: Pharmaceutical Sciences Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.|Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors.forename: Mohammad Hosein
authors.initials: MH
authors.lastname: Farzaei
authors.affiliation: Pharmaceutical Sciences Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran gbahrami@kums.ac.ir.|Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors.forename: Gholamreza
authors.initials: G
authors.lastname: Bahrami
mesh_terms: D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003920:Diabetes Mellitus / Q000188:drug therapy*; D005638:Fruit / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D007004:Hypoglycemic Agents / Q000627:therapeutic use* / Q000633:toxicity; D008519:Medicine, Traditional; D010936:Plant Extracts / Q000627:therapeutic use* / Q000633:toxicity; D027826:Rosa / Q000737:chemistry*
chemical_list: D007004:Hypoglycemic Agents; D010936:Plant Extracts
keywords: Rosa canina; cell proliferation; diabetes mellitus; traditional medicine