title: Chikusetsu saponin V attenuates H2O2-induced oxidative stress in human neuroblastoma SH-SY5Y cells through Sirt1/PGC-1α/Mn-SOD signaling pathways.
abstract: Oxidative stress plays a vital role in the pathogenesis of neurodegenerative diseases. Chikusetsu saponin V (CsV), the most abundant member of saponins from Panax japonicus (SPJ), has attracted increasing attention for its potential to treat neurodegenerative diseases. However, the mechanisms are unclear. Our study intended to investigate the antioxidative effects of CsV in human neuroblastoma SH-SY5Y cells. Our data showed that CsV attenuated H2O2-induced cytotoxicity, inhibited ROS accumulation, increased the activities of superoxide dismutase (SOD) and GSH, and increased mitochondrial membrane potential dose-dependently. Further exploration of the mechanisms showed that CsV exhibited these effects through increasing the activation of oxidative-stress-associated factors including Sirt1, PGC-1α, and Mn-SOD. Moreover, CsV inhibited H2O2-induced down-regulation of Bcl-2 and up-regulation of Bax in a dose-dependent manner and, thus, increased the ratio of Bcl-2/Bax. In conclusion, our study demonstrated that CsV exhibited neuroprotective effects possibly through Sirt1/PGC-1α/Mn-SOD signaling pathways.
authors.affiliation: a College of Medical Science, Three Gorges University, Yichang, Hubei 443002, China.
authors.forename: Jingzhi
authors.initials: J
authors.lastname: Wan
authors.affiliation: a College of Medical Science, Three Gorges University, Yichang, Hubei 443002, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Deng
authors.affiliation: a College of Medical Science, Three Gorges University, Yichang, Hubei 443002, China.
authors.forename: Changcheng
authors.initials: C
authors.lastname: Zhang
authors.affiliation: a College of Medical Science, Three Gorges University, Yichang, Hubei 443002, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Yuan
authors.affiliation: a College of Medical Science, Three Gorges University, Yichang, Hubei 443002, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.affiliation: a College of Medical Science, Three Gorges University, Yichang, Hubei 443002, China.
authors.forename: Yaoyan
authors.initials: Y
authors.lastname: Dun
authors.affiliation: a College of Medical Science, Three Gorges University, Yichang, Hubei 443002, China.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: a College of Medical Science, Three Gorges University, Yichang, Hubei 443002, China.
authors.forename: Haixia
authors.initials: H
authors.lastname: Zhao
authors.affiliation: b Three Gorges University Institute of Molecular Biology, Yichang, Hubei 443002, China.
authors.forename: Chaoqi
authors.initials: C
authors.lastname: Liu
authors.affiliation: c Renhe Hospital, The second College of Clinical Medical Science, Three Gorges University, Yichang, Hubei 443001, China.
authors.forename: Ding
authors.initials: D
authors.lastname: Yuan
authors.affiliation: a College of Medical Science, Three Gorges University, Yichang, Hubei 443002, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D001549:Benzamides / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D009284:Naphthols / Q000494:pharmacology; D009447:Neuroblastoma / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012503:Saponins / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects*; D056564:Sirtuin 1 / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D001549:Benzamides; D009284:Naphthols; C486497:PPARGC1A protein, human; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D017382:Reactive Oxygen Species; D012503:Saponins; C588229:chikusetsusaponin V; C439060:sirtinol; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; D013482:Superoxide Dismutase; C447939:SIRT1 protein, human; D056564:Sirtuin 1; D005978:Glutathione
keywords: Chikusetsu saponin V; Mn-SOD; PGC-1α; SH-SY5Y cells; Sirt1; cellules SH-SY5Y; saponine Chikusetsu V