title: Localization of corin and atrial natriuretic peptide expression in human renal segments.
abstract: Atrial natriuretic peptide (ANP)-mediated natriuretic response is a well-established cardiac endocrine function. Corin is a transmembrane protease that activates ANP in the heart. Corin expression has been detected in non-cardiac tissues including the kidney. Here we examined corin, pro-ANP/ANP and natriuretic peptide receptor-A (NPR-A) expression in human renal segments. By immunostaining and in situ hybridization, we found similar corin, pro-ANP/ANP and NPR-A protein and mRNA expression in human renal segments. The expression was most abundant in the proximal convoluted tubules and the medullary connecting ducts. In the proximal tubules, corin protein was present in the apical membrane region underneath the brush border where the ANP-degrading protease neprilysin was abundant. These results suggest that corin-mediated pro-ANP activation may occur in renal segments and that locally produced ANP may act in an autocrine manner to regulate sodium and water reabsorption in situ Our results also point to the proximal convoluted tubules as a major site for local ANP action. Such a renal corin/ANP autocrine mechanism may differ from the cardiac corin/ANP endocrine mechanism in regulating sodium homoeostasis under physiological and pathological conditions.
authors.affiliation: Cyrus Tang Hematology Center and Collaborative Innovation Center of Hematology, Soochow University, Suzhou, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Dong
authors.affiliation: Molecular Cardiology, Cleveland Clinic, Cleveland, U.S.A.
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.affiliation: Cyrus Tang Hematology Center and Collaborative Innovation Center of Hematology, Soochow University, Suzhou, China Thrombosis and Hemostasis Key Laboratory, Ministry of Education Engineering Center for Hematological Disease, Jiangsu Institute of Hematology, the First Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: Ningzheng
authors.initials: N
authors.lastname: Dong
authors.affiliation: Cyrus Tang Hematology Center and Collaborative Innovation Center of Hematology, Soochow University, Suzhou, China.
authors.forename: Ce
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Urology Department, the Second Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: Boxin
authors.initials: B
authors.lastname: Xue
authors.affiliation: Cyrus Tang Hematology Center and Collaborative Innovation Center of Hematology, Soochow University, Suzhou, China Molecular Cardiology, Cleveland Clinic, Cleveland, U.S.A. wuq@ccf.org.
authors.forename: Qingyu
authors.initials: Q
authors.lastname: Wu
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D007668:Kidney / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D020098:Natriuretic Peptide, C-Type / Q000235:genetics / Q000378:metabolism; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport; D017461:Receptors, Atrial Natriuretic Factor / Q000378:metabolism; D012697:Serine Endopeptidases / Q000235:genetics / Q000378:metabolism*
chemical_list: C472955:NPPA protein, human; C431578:Nppa protein, mouse; D011498:Protein Precursors; D020098:Natriuretic Peptide, C-Type; D009320:Atrial Natriuretic Factor; C119812:CORIN protein, human; C485617:Corin protein, mouse; D012697:Serine Endopeptidases; D017461:Receptors, Atrial Natriuretic Factor; C091715:atrial natriuretic factor receptor A
keywords: atrial natriuretic peptide; corin; natriuretic peptide receptor; neprilysin