title: Bioinformatics investigation of therapeutic mechanisms of Xuesaitong capsule treating ischemic cerebrovascular rat model with comparative transcriptome analysis.
abstract: BACKGROUND
  Xuesaitong soft capsule (XST) which consists of panax notoginseng saponin (PNS) has been used to treat ischemic cerebrovascular diseases in China. The therapeutic mechanism of XST has not been elucidated yet from prospective of genomics and bioinformatics.
  
  
  METHODS
  A transcriptome analysis was performed to review series concerning middle cerebral artery occlusion (MCAO) rat model and XST intervention after MCAO from Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) were compared between blank group and model group, model group and XST group. Functional enrichment and pathway analysis were performed. Protein-Protein interaction network was constructed. The overlapping genes from two DEGs sets were screened out and profound analysis was performed.
  
  
  RESULTS
  Two series including 22 samples were obtained. 870 DEGs were identified between blank group and model group, and 1189 DEGs were identified between model group and XST group. GO terms and KEGG pathways of MCAO and XST intervention were significantly enriched. PPI networks were constructed to demonstrate the gene-gene interactions. The overlapping genes from two DEGs sets were highlighted. ANTXR2, FHL3, PRCP, TYROBP, TAF9B, FGFR2, BCL11B, RB1CC1 and MBNL2 were the pivotal genes and possible action sites of XST therapeutic mechanisms.
  
  
  CONCLUSIONS
  MCAO is a pathological process with multiple.
authors.affiliation: Guang'anmen Hospital, China Academy of Chinese Medical SciencesNo. 5 Beixiange Street, Xicheng District, Beijing 100053, China; Graduate School, Beijing University of Chinese MedicineNo. 11 Beisanhuan East Road, Chaoyang District, Beijing 100029, China.
authors.forename: Jiangquan
authors.initials: J
authors.lastname: Liao
authors.affiliation: Hubei University of Chinese Medicine No. 1 Huangjiahu West Road, Hongshan District, Wuhan 430065, China.
authors.forename: Benjun
authors.initials: B
authors.lastname: Wei
authors.affiliation: Guang'anmen Hospital, China Academy of Chinese Medical Sciences No. 5 Beixiange Street, Xicheng District, Beijing 100053, China.
authors.forename: Hengwen
authors.initials: H
authors.lastname: Chen
authors.affiliation: Guang'anmen Hospital, China Academy of Chinese Medical Sciences No. 5 Beixiange Street, Xicheng District, Beijing 100053, China.
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Guang'anmen Hospital, China Academy of Chinese Medical Sciences No. 5 Beixiange Street, Xicheng District, Beijing 100053, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
keywords: Middle cerebral artery occlusion; Traditional Chinese medicine; Xuesaitong capsule; bioinformatics; transcriptome analysis