title: Epidemiology and transmission characteristics of human adenovirus type 7 caused acute respiratory disease outbreak in military trainees in East China.
abstract: BACKGROUND
  Human adenovirus type 7 (HAdV7) is globally attracting great concern as its high morbidity and severity in respiratory diseases, especially in Asia.
  
  
  OBJECTIVE
  To investigate the clinical and epidemiologic characteristics of HAdV7 infection outbreak in East China.
  
  
  METHODS
  The clinical samples were collected from the patients of an ARD outbreak in East Chinafor the detection of causative pathogens by multiplex PCR. The molecular type of human adenovirus isolates were identified by sequencing and homologous comparison based on their hexon genes. The spatiotemporal dynamics of global HAdV7 was investigated using the phylogenetic and phylogeographic analyses. Total 67 referenced HAdV7 hexon sequences (>800 bp) from GenBank were selected for constructing the maximum likelihood tree by MEGA 5.1.0, grouped according to the tree topology for the further migration analysis by PAUP* 4.0 and MigraPhyla 1.0 b to understand the transmission patterns of HAdV7 in global epidemics.
  
  
  RESULTS
  The results showed HAdV7 as the causative pathogen in this outbreak, and the outbreak strains had the hexon sequences highly identical with the isolates in Shaanxi (2012). The origin of HAdV7 was inferred as California, meanwhile a total of 21 migration routes were acquired. HAdV7 in this outbreak was statistically proven dispersed from Shaanxi province (2012).
  
  
  CONCLUSIONS
  The analyses of epidemiology and transmission pattern of HAdV7 would not only enrich the molecular biological basic database but also provide theoretical basis for HAdV7 prevention and control strategy.
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou, China; Department of Clinical Laboratory Science, The 117th Hospital of PLAHangzhou,China.
authors.forename: Jun
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Department of Respiratory Medicine, The 117th Hospital of PLA Hangzhou, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Qi
authors.affiliation: Department of Respiratory Medicine, The 117th Hospital of PLA Hangzhou, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Biotechnology, The University of Tokyo Tokyo, Japan.
authors.forename: Xujian
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Clinical Laboratory Science, The 117th Hospital of PLA Hangzhou,China.
authors.forename: Guozheng
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory Science, The 117th Hospital of PLA Hangzhou,China.
authors.forename: Yuzhu
authors.initials: Y
authors.lastname: Dai
authors.affiliation: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, College of Medicine, Zhejiang University Hangzhou, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Cui
authors.affiliation: Department of Respiratory Medicine, The 117th Hospital of PLA Hangzhou, China.
authors.forename: Qingyong
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Department of Infectious Diseases, The 113rd Hospital of PLA Ningbo, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Fan
authors.affiliation: Department of Infectious Diseases, The 85th Hospital of PLA Shanghai, China.
authors.forename: Liuda
authors.initials: L
authors.lastname: Ni
authors.affiliation: Department of Radio Diagnosis and Imaging, The 117th Hospital of PLA Hangzhou, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Infectious Diseases, The 117th Hospital of PLA Hangzhou, China.
authors.forename: Feiyan
authors.initials: F
authors.lastname: Zhu
authors.affiliation: Department of Epidemiology and Infection Control, The 117th Hospital of PLA Hangzhou, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Yang
authors.affiliation: Institute of Military Medical Sciences of Nanjing Military Command Nanjing, China.
authors.forename: Changjun
authors.initials: C
authors.lastname: Wang
authors.affiliation: Center for Disease Control and Prevention of Nanjing Military Command Nanjing, China.
authors.forename: Yuexi
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratory Science, The 117th Hospital of PLA Hangzhou,China.
authors.forename: Changgui
authors.initials: C
authors.lastname: Sun
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical University Wenzhou, China.
authors.forename: Zhongyong
authors.initials: Z
authors.lastname: Wang
keywords: Human adenovirus type 7; clinical characteristics; epidemiology; phylogeny; phylogeography