title: Mg deficiency affects leaf Mg remobilization and the proteome in Brassica napus.
abstract: In order to cope with variable mineral nutrient availability, higher plants have developed numerous strategies including the remobilization of nutrients from source to sink tissues. However, such processes remain relatively unknown for magnesium (Mg), which is the third most important cation in plant tissues. Using Mg depletion of Brassica napus, we have demonstrated that Mg is remobilized from old leaves to young shoot tissues. Moreover, this study showed that Mg depletion induces modification of nutrient uptake, especially Zn and Mn. Finally, comparative proteomic analysis of old leaves (source of Mg) revealed amongst other results that some proteins requiring Mg for their functionality (isocitrate dehydrogenase for example) were up-regulated. Moreover, down-regulation of proteases suggested that mobilization of Mg from old leaves was not associated with senescence.
authors.affiliation: Normandie Université, Caen, France; UNICAEN, UMR 950 Ecophysiologie Végétale, Agronomie et Nutritions N, C, S, Esplanade de la Paix, CS14032, 14032, Caen Cedex 5, France; INRA, UMR 950 Ecophysiologie Végétale, Agronomie et Nutritions N, C, S, Esplanade de la Paix, CS14032, 14032, Caen Cedex 5, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Billard
authors.affiliation: Normandie Université, Caen, France; UNICAEN, UMR 950 Ecophysiologie Végétale, Agronomie et Nutritions N, C, S, Esplanade de la Paix, CS14032, 14032, Caen Cedex 5, France; INRA, UMR 950 Ecophysiologie Végétale, Agronomie et Nutritions N, C, S, Esplanade de la Paix, CS14032, 14032, Caen Cedex 5, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Maillard
authors.affiliation: Plateforme de Protéomique PISSARO, UMR6270 CNRS Faculté des Sciences de Rouen, 76821, Mont-Saint-Aignan, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Coquet
authors.affiliation: Plateforme de Protéomique PISSARO, UMR6270 CNRS Faculté des Sciences de Rouen, 76821, Mont-Saint-Aignan, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Jouenne
authors.affiliation: Agro Innovation International, Centre Mondial d'Innovation, Groupe Roullier, 18 Avenue Franklin Roosevelt, BP 70158, 35401, Saint Malo Cedex, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Cruz
authors.affiliation: Timac Agro Spain, Poligono de Arazuri-Orcoyen Calle C n°32, 31160, Orcoyen, Spain.
authors.forename: José-Maria
authors.initials: JM
authors.lastname: Garcia-Mina
authors.affiliation: Agro Innovation International, Centre Mondial d'Innovation, Groupe Roullier, 18 Avenue Franklin Roosevelt, BP 70158, 35401, Saint Malo Cedex, France.
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Yvin
authors.affiliation: Normandie Université, Caen, France; UNICAEN, UMR 950 Ecophysiologie Végétale, Agronomie et Nutritions N, C, S, Esplanade de la Paix, CS14032, 14032, Caen Cedex 5, France; INRA, UMR 950 Ecophysiologie Végétale, Agronomie et Nutritions N, C, S, Esplanade de la Paix, CS14032, 14032, Caen Cedex 5, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Ourry
authors.affiliation: Normandie Université, Caen, France; UNICAEN, UMR 950 Ecophysiologie Végétale, Agronomie et Nutritions N, C, S, Esplanade de la Paix, CS14032, 14032, Caen Cedex 5, France; INRA, UMR 950 Ecophysiologie Végétale, Agronomie et Nutritions N, C, S, Esplanade de la Paix, CS14032, 14032, Caen Cedex 5, France. Electronic address: philippe.etienne@unicaen.fr.
authors.forename: Philippe
authors.initials: P
authors.lastname: Etienne
mesh_terms: D018533:Biomass; D029688:Brassica napus / Q000254:growth & development / Q000378:metabolism*; D015536:Down-Regulation / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D008274:Magnesium / Q000378:metabolism*; D008903:Minerals / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D015854:Up-Regulation / Q000235:genetics
chemical_list: D008903:Minerals; D010940:Plant Proteins; D020543:Proteome; D008274:Magnesium
keywords: Magnesium; Manganese; Mineral nutrition; Proteomic analysis