title: Effects of the Food Manufacturing Chain on the Viability and Functionality of Bifidobacterium animalis through Simulated Gastrointestinal Conditions.
abstract: The viability and functionality of probiotics may be influenced by industrial production processes resulting in a decrease in probiotic efficiency that benefit the health of humans. This study aimed to investigate the probiotic characteristics of Bifidobacterium strains isolated from fecal samples of healthy Thai infants. In the present work, three local strains (BF014, BF052, and BH053) belonging to Bifidobacterium animalis showed a great resistance against conditions simulating the gastrointestinal tract. Among these, B. animalis BF052 possessed considerable probiotic properties, including high acid and bile tolerance, strong adhesion capability to Caco-2 cells, and inhibitory activity against pathogens including Salmonella typhimurium and Vibrio cholerae. This strain also exhibited a high survival rate compared to commercial strains during storage in a wide variety of products, including pasteurized milk, soy milk, drinking yogurt, and orange juice. The impact of food processing processes as well as the freeze-drying process, storage of freeze-dried powders, and incorporation of freeze-dried cells in food matrix on probiotic properties was also determined. The stability of the probiotic properties of the BF052 strain was not affected by food processing chain, especially its resistance in the simulated gastrointestinal conditions and its adherence ability to Caco-2 cells. It indicates that it satisfies the criteria as a potential probiotic and may be used as an effective probiotic starter in food applications.
authors.affiliation: Metabolic Engineering Research Unit, Institute of Agricultural Technology, School of Biotechnology, Suranaree University of Technology, 111 University Avenue, Suranaree Sub-district, Muang District, Nakhon Ratchasima 30000, Thailand.
authors.forename: Pattra
authors.initials: P
authors.lastname: Charnchai
authors.affiliation: Division of Biopharmacy, Faculty of Pharmaceutical Sciences, Ubon Ratchathani University, Warinchamrap, Ubon Ratchathani 34190, Thailand.
authors.forename: Sirima Suvarnakuta
authors.initials: SS
authors.lastname: Jantama
authors.affiliation: Division of Biopharmacy, Faculty of Pharmaceutical Sciences, Ubon Ratchathani University, Warinchamrap, Ubon Ratchathani 34190, Thailand.
authors.forename: Chutinun
authors.initials: C
authors.lastname: Prasitpuriprecha
authors.affiliation: Metabolic Engineering Research Unit, Institute of Agricultural Technology, School of Biotechnology, Suranaree University of Technology, 111 University Avenue, Suranaree Sub-district, Muang District, Nakhon Ratchasima 30000, Thailand.
authors.forename: Sunthorn
authors.initials: S
authors.lastname: Kanchanatawee
authors.affiliation: Metabolic Engineering Research Unit, Institute of Agricultural Technology, School of Biotechnology, Suranaree University of Technology, 111 University Avenue, Suranaree Sub-district, Muang District, Nakhon Ratchasima 30000, Thailand.
authors.forename: Kaemwich
authors.initials: K
authors.lastname: Jantama
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001422:Bacterial Adhesion / Q000187:drug effects; D000069982:Bifidobacterium animalis / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D001647:Bile Acids and Salts / Q000494:pharmacology; D018938:Caco-2 Cells; D002448:Cell Adhesion / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003451:Cryoprotective Agents / Q000494:pharmacology; D005502:Food; D005612:Freeze Drying; D041981:Gastrointestinal Tract / Q000187:drug effects / Q000382:microbiology*; D005772:Gastrointestinal Transit / Q000187:drug effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007223:Infant; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D010194:Pancreatin / Q000494:pharmacology; D019936:Probiotics / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001647:Bile Acids and Salts; D003451:Cryoprotective Agents; D010194:Pancreatin