title: A rhesus macaque model of Asian-lineage Zika virus infection.
abstract: Infection with Asian-lineage Zika virus (ZIKV) has been associated with Guillain-Barré syndrome and fetal abnormalities, but the underlying mechanisms remain poorly understood. Animal models of infection are thus urgently needed. Here we show that rhesus macaques are susceptible to infection by an Asian-lineage ZIKV closely related to strains currently circulating in the Americas. Following subcutaneous inoculation, ZIKV RNA is detected in plasma 1 day post infection (d.p.i.) in all animals (N=8, including 2 pregnant animals), and is also present in saliva, urine and cerebrospinal fluid. Non-pregnant and pregnant animals remain viremic for 21 days and for up to at least 57 days, respectively. Neutralizing antibodies are detected by 21 d.p.i. Rechallenge 10 weeks after the initial challenge results in no detectable virus replication, indicating protective immunity against homologous strains. Therefore, Asian-lineage ZIKV infection of rhesus macaques provides a relevant animal model for studying pathogenesis and evaluating potential interventions against human infection, including during pregnancy.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA.
authors.forename: Dawn M
authors.initials: DM
authors.lastname: Dudley
authors.affiliation: Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
authors.forename: Matthew T
authors.initials: MT
authors.lastname: Aliota
authors.affiliation: Department of Pediatrics, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA.
authors.forename: Emma L
authors.initials: EL
authors.lastname: Mohr
authors.affiliation: Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53715, USA.
authors.forename: Andrea M
authors.initials: AM
authors.lastname: Weiler
authors.affiliation: Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53715, USA.
authors.forename: Gabrielle
authors.initials: G
authors.lastname: Lehrer-Brey
authors.affiliation: Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53715, USA.
authors.forename: Kim L
authors.initials: KL
authors.lastname: Weisgrau
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA.
authors.forename: Mariel S
authors.initials: MS
authors.lastname: Mohns
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA.
authors.forename: Meghan E
authors.initials: ME
authors.lastname: Breitbach
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA.
authors.forename: Mustafa N
authors.initials: MN
authors.lastname: Rasheed
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA.
authors.forename: Christina M
authors.initials: CM
authors.lastname: Newman
authors.affiliation: Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53715, USA.
authors.forename: Dane D
authors.initials: DD
authors.lastname: Gellerup
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA.|Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
authors.forename: Louise H
authors.initials: LH
authors.lastname: Moncla
authors.affiliation: Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53715, USA.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Post
authors.affiliation: Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53715, USA.
authors.forename: Nancy
authors.initials: N
authors.lastname: Schultz-Darken
authors.affiliation: Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53715, USA.
authors.forename: Michele L
authors.initials: ML
authors.lastname: Schotzko
authors.affiliation: Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53715, USA.
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Hayes
authors.affiliation: Department of Pediatrics and Human Vaccine Institute, Duke University Medical Center, Durham, North Carolina 27710, USA.
authors.forename: Josh A
authors.initials: JA
authors.lastname: Eudailey
authors.affiliation: Department of Pediatrics and Human Vaccine Institute, Duke University Medical Center, Durham, North Carolina 27710, USA.
authors.forename: M Anthony
authors.initials: MA
authors.lastname: Moody
authors.affiliation: Department of Pediatrics and Human Vaccine Institute, Duke University Medical Center, Durham, North Carolina 27710, USA.
authors.forename: Sallie R
authors.initials: SR
authors.lastname: Permar
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA.
authors.forename: Shelby L
authors.initials: SL
authors.lastname: O'Connor
authors.affiliation: Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53715, USA.
authors.forename: Eva G
authors.initials: EG
authors.lastname: Rakasz
authors.affiliation: Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53715, USA.
authors.forename: Heather A
authors.initials: HA
authors.lastname: Simmons
authors.affiliation: Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53715, USA.
authors.forename: Saverio
authors.initials: S
authors.lastname: Capuano
authors.affiliation: Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53715, USA.|Department of Comparative Biosciences and Obstetrics and Gynecology, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
authors.forename: Thaddeus G
authors.initials: TG
authors.lastname: Golos
authors.affiliation: Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
authors.forename: Jorge E
authors.initials: JE
authors.lastname: Osorio
authors.affiliation: Department of Pathobiological Sciences, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.|Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53715, USA.
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Friedrich
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA.|Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53715, USA.
authors.forename: David H
authors.initials: DH
authors.lastname: O'Connor
mesh_terms: D056704:Adaptive Immunity; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D007113:Immunity, Innate; D008253:Macaca mulatta; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000276:immunology / Q000821:virology*; D000071244:Zika Virus; D000071243:Zika Virus Infection / Q000276:immunology / Q000821:virology*