title: MiR-410 Down-Regulates the Expression of Interleukin-10 by Targeting STAT3 in the Pathogenesis of Systemic Lupus Erythematosus.
abstract: OBJECTIVE
  Systemic lupus erythematosus (SLE) is a heterogeneous chronic inflammatory autoimmune disorder, in the pathogenesis of which miRNAs play a versatile function. The purpose of this study was to investigate the effect of miRNA-410 on the pathogenesis of SLE in T cells of SLE patients.
  
  
  METHODS
  Real-time PCR was used to test the mRNA levels of miRNA-410 in SLE patients and healthy controls. ELISA analysis was performed to examine the production levels of IL-10. Luciferase Assay was used to confirm the targeting effect of miRNA-410 on 3'UTR of STAT3 mRNA.
  
  
  RESULTS
  We found that the expression level of miR-410 in T cells of SLE patients was decreased comparing to that in healthy controls, whereas overexpression of miR-410 significantly reduced the expression levels of IL-10. Furthermore, miR-410 suppresses the transcription activity of STAT3 by binding directly to the 3 'UTR of STAT3 mRNA. Moreover, silence of STAT3 down regulated IL-10 expression in CD3+ T cells.
  
  
  CONCLUSIONS
  Our results demonstrate that miR-410 is the key regulatory factor in the pathogenesis of SLE by regulating the expression of IL-10 through targeting STAT3. These data suggest a novel function of miR-410 and bring new insight into understanding the complex mechanisms involved in SLE.
authors.affiliation: Department of Rheumatology and Immunology, Shengjing Hospital of China Medical University, Shenyang, People's Republic of China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Liu
authors.forename: Na
authors.initials: N
authors.lastname: Zhang
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Zhang
authors.forename: Muting
authors.initials: M
authors.lastname: Qin
authors.forename: Youdan
authors.initials: Y
authors.lastname: Dong
authors.forename: Lei
authors.initials: L
authors.lastname: Jin
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D001483:Base Sequence; D015153:Blotting, Western; D002478:Cells, Cultured; D015536:Down-Regulation; D005786:Gene Expression Regulation; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics* / Q000378:metabolism; D008180:Lupus Erythematosus, Systemic / Q000235:genetics* / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics*; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050796:STAT3 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D013601:T-Lymphocytes / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; C508609:IL10 protein, human; C568527:MIRN410 microRNA, human; D035683:MicroRNAs; D050796:STAT3 Transcription Factor; D016753:Interleukin-10