title: Enhanced production of resveratrol derivatives in tobacco plants by improving the metabolic flux of intermediates in the phenylpropanoid pathway.
abstract: The biosynthesis of flavonoids such as anthocyanin and stilbenes has attracted increasing attention because of their potential health benefits. Anthocyanins and stilbenes share common phenylpropanoid precursor pathways. We previously reported that the overexpression of sweetpotato IbMYB1a induced anthocyanin pigmentation in transgenic tobacco (Nicotiana tabacum) plants. In the present study, transgenic tobacco (Nicotiana tabacum SR1) plants (STS-OX and ROST-OX) expressing the RpSTS gene encoding stilbene synthase from rhubarb (Rheum palmatum L. cv. Jangyeop) and the RpSTS and VrROMT genes encoding resveratrol O-methyltransferase from frost grape (Vitis riparia) were generated under the control of 35S promoter. Phenotypic alterations in floral organs, such as a reduction in floral pigments and male sterility, were observed in STS-OX transgenic tobacco plants. However, we failed to obtain STS-OX and ROST-OX plants with high levels of resveratrol compounds. Therefore, to improve the production of resveratrol derivatives in plants, we cross-pollinated flowers of STS-OX or ROST-OX and IbMYB1a-OX transgenic lines (SM and RSM). Phenotypic changes in vegetative and reproductive development of SM and RSM plants were observed. Furthermore, by HPLC and LC-MS analyses, we found enhanced production of resveratrol derivatives such as piceid, piceid methyl ether, resveratrol methyl ether O-hexoside, and 5-methyl resveratrol-3,4'-O-β-D-diglucopyranoside in SM and RSM cross-pollinated lines. Here, total contents of trans- and cis-piceids ranged from approximately 104-240 µg/g fresh weight in SM (F2). Collectively, we suggest that coexpression of RpSTS and IbMYB1a via cross-pollination can induce enhanced production of resveratrol compounds in plants by increasing metabolic flux into stilbenoid biosynthesis.
authors.affiliation: Natural Product Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, 580-185, Republic of Korea.
authors.forename: Yu Jeong
authors.initials: YJ
authors.lastname: Jeong
authors.affiliation: Natural Product Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, 580-185, Republic of Korea.|Department of Bioscience and Biotechnology, Chungnam National University, Daejeon, 305-806, Republic of Korea.
authors.forename: Chul Han
authors.initials: CH
authors.lastname: An
authors.affiliation: Natural Product Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, 580-185, Republic of Korea.
authors.forename: Su Gyeong
authors.initials: SG
authors.lastname: Woo
authors.affiliation: Natural Product Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, 580-185, Republic of Korea.
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Park
authors.affiliation: Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration, Cheonan, 331-801, Republic of Korea.
authors.forename: Ki-Won
authors.initials: KW
authors.lastname: Lee
authors.affiliation: Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration, Cheonan, 331-801, Republic of Korea.
authors.forename: Sang-Hoon
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Division of Applied Life Science (BK21plus), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju, 660-701, Republic of Korea.
authors.forename: Yeonggil
authors.initials: Y
authors.lastname: Rim
authors.affiliation: Natural Product Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, 580-185, Republic of Korea.
authors.forename: Hyung Jae
authors.initials: HJ
authors.lastname: Jeong
authors.affiliation: Natural Product Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, 580-185, Republic of Korea.
authors.forename: Young Bae
authors.initials: YB
authors.lastname: Ryu
authors.affiliation: Natural Product Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, 580-185, Republic of Korea. kimcy@kribb.re.kr.
authors.forename: Cha Young
authors.initials: CY
authors.lastname: Kim
mesh_terms: D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D054817:Pollination / Q000235:genetics / Q000502:physiology; D000077185:Resveratrol; D013267:Stilbenes / Q000378:metabolism*; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D013267:Stilbenes; D000077185:Resveratrol
keywords: Anthocyanin; Cross-pollination; Metabolic engineering; Resveratrol; Stilbene; Tobacco