title: Metagenomic analysis of bacterial community composition and antibiotic resistance genes in a wastewater treatment plant and its receiving surface water.
abstract: The presence of pathogenic bacteria and the dissemination of antibiotic resistance genes (ARGs) may pose big risks to the rivers that receive the effluent from municipal wastewater treatment plants (WWTPs). In this study, we investigated the changes of bacterial community and ARGs along treatment processes of one WWTP, and examined the effects of the effluent discharge on the bacterial community and ARGs in the receiving river. Pyrosequencing was applied to reveal bacterial community composition including potential bacterial pathogen, and Illumina high-throughput sequencing was used for profiling ARGs. The results showed that the WWTP had good removal efficiency on potential pathogenic bacteria (especially Arcobacter butzleri) and ARGs. Moreover, the bacterial communities of downstream and upstream of the river showed no significant difference. However, the increase in the abundance of potential pathogens and ARGs at effluent outfall was observed, indicating that WWTP effluent might contribute to the dissemination of potential pathogenic bacteria and ARGs in the receiving river.
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, Environmental Health Research Center, School of the Environment, Nanjing University, Nanjing 210023, China.
authors.forename: Junying
authors.initials: J
authors.lastname: Tang
authors.affiliation: Key Laboratory of Pesticide Environmental Assessment and Pollution Control, Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection of China, Nanjing, China.
authors.forename: Yuanqing
authors.initials: Y
authors.lastname: Bu
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, Environmental Health Research Center, School of the Environment, Nanjing University, Nanjing 210023, China. Electronic address: zhangxx@nju.edu.cn.
authors.forename: Xu-Xiang
authors.initials: XX
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, Environmental Health Research Center, School of the Environment, Nanjing University, Nanjing 210023, China.
authors.forename: Kailong
authors.initials: K
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, Environmental Health Research Center, School of the Environment, Nanjing University, Nanjing 210023, China.
authors.forename: Xiwei
authors.initials: X
authors.lastname: He
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, Environmental Health Research Center, School of the Environment, Nanjing University, Nanjing 210023, China. Electronic address: linye@nju.edu.cn.
authors.forename: Lin
authors.initials: L
authors.lastname: Ye
authors.affiliation: Key Laboratory of Pesticide Environmental Assessment and Pollution Control, Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection of China, Nanjing, China.
authors.forename: Zhengjun
authors.initials: Z
authors.lastname: Shan
authors.affiliation: State Key Laboratory of Pollution Control and Resource Reuse, Environmental Health Research Center, School of the Environment, Nanjing University, Nanjing 210023, China.
authors.forename: Hongqiang
authors.initials: H
authors.lastname: Ren
mesh_terms: D000900:Anti-Bacterial Agents / Q000633:toxicity*; D001419:Bacteria / Q000187:drug effects / Q000235:genetics; D004269:DNA, Bacterial; D004352:Drug Resistance, Microbial / Q000235:genetics*; D004784:Environmental Monitoring / Q000379:methods; D005798:Genes, Bacterial; D056186:Metagenomics / Q000379:methods*; D045483:Rivers / Q000382:microbiology*; D062065:Wastewater / Q000382:microbiology*; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D018508:Water Purification / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Antibiotic resistance genes; High-throughput sequencing; Microbial community; Pathogenic bacteria; Receiving surface water