title: Effects of NMDA-Receptor Antagonist on the Expressions of Bcl-2 and Bax in the Subventricular Zone of Neonatal Rats with Hypoxia-Ischemia Brain Damage.
abstract: Neonatal hypoxia-ischemia brain damage is an important cause of death by affecting prognosis of neural diseases. It is difficult to find effective methods of prevention and treatment due to the complexity of its pathogenesis. N-methyl-D-aspartate (NMDA), as an excitotoxicity amino acids, has proven to play an important role in hypoxic-ischemic. However, the exact effects of the NMDA subunits, NR2A and NR2B, during hypoxic-ischemic have not been investigated in detail. Therefore, we sought to study whether the NMDA receptor antagonist could confer neuroprotective effects in a neonatal rat hypoxia-ischemia model. The effects of intraperitoneal injections of different drugs, namely MK-801 (0.5 mg/kg), NVP-AAM077 (5 mg/kg), and Ro25-6981 (5 mg/kg), on the expressions of anti-apoptotic protein Bcl-2 and apoptosis protein Bax in the subventricular zone were analyzed by immunohistochemical staining to explore the roles of NMDA subunits (NR2A and NR2B) in hypoxic-ischemic. We found that the NR2B antagonist (Ro25-6981) could inhibit hypoxic-ischemic with the increasing Bcl-2 expression. NR2A antagonists (NVP-AAM077) can increase cerebral hypoxia-ischemia in neonatal rats, promoting the expression of apoptotic protein Bax.
authors.affiliation: Department of Neurology, Affiliated Hospital of Xuzhou Medical College, Xuzhou, 221002, Jiangsu, China.
authors.forename: Hongbin
authors.initials: H
authors.lastname: Fan
authors.affiliation: Department of Neurology, Affiliated Hospital of Xuzhou Medical College, Xuzhou, 221002, Jiangsu, China.
authors.forename: Xiaoquan
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Neurology, Affiliated Hospital of Xuzhou Medical College, Xuzhou, 221002, Jiangsu, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Neurology, Affiliated Hospital of Xuzhou Medical College, Xuzhou, 221002, Jiangsu, China.
authors.forename: Qingwei
authors.initials: Q
authors.lastname: Lai
authors.affiliation: Department of Neurology, Affiliated Hospital of Xuzhou Medical College, Xuzhou, 221002, Jiangsu, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Tang
authors.affiliation: Department of Anatomy and Neurobiology, Xuzhou Medical College, 84 West Huaihai Road, Xuzhou, 221004, Jiangsu, China.
authors.forename: Dianshuai
authors.initials: D
authors.lastname: Gao
authors.affiliation: Department of Clinical Pharmacology, School of Pharmacy, Xuzhou Medical College, 84 West Huaihai Road, Xuzhou, 221004, Jiangsu, China. yinxx@xzmc.edu.cn.
authors.forename: Xiaoxing
authors.initials: X
authors.lastname: Yin
authors.affiliation: Department of Anatomy and Neurobiology, Xuzhou Medical College, 84 West Huaihai Road, Xuzhou, 221004, Jiangsu, China. 1020858568@qq.com.
authors.forename: Tiejun
authors.initials: T
authors.lastname: Xu
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D016291:Dizocilpine Maleate / Q000494:pharmacology; D020925:Hypoxia-Ischemia, Brain / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D007150:Immunohistochemistry; D020547:Lateral Ventricles / Q000378:metabolism*; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D010636:Phenols / Q000494:pharmacology; D010880:Piperidines / Q000494:pharmacology; D021122:Protein Subunits / Q000037:antagonists & inhibitors / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D011810:Quinoxalines / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors* / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism*
chemical_list: C498554:5-(alpha-methyl-4-bromobenzylamino)phosphonomethyl-1,4-dihydroquinoxaline-2,3-dione; D018696:Neuroprotective Agents; D010636:Phenols; D010880:Piperidines; D021122:Protein Subunits; D019253:Proto-Oncogene Proteins c-bcl-2; D011810:Quinoxalines; D016194:Receptors, N-Methyl-D-Aspartate; C109643:Ro 25-6981; D051028:bcl-2-Associated X Protein; D016291:Dizocilpine Maleate
keywords: Apoptosis; Bax; Bcl-2; Hypoxia–ischemia; NMDA receptor antagonist