title: Molecular Typing of Hospital-Acquired Staphylococcus aureus Isolated from Isfahan, Iran.
abstract: Background. Staphylococcus aureus (S. aureus) is one of the most common pathogens that cause hospital- and community-acquired infections in the world. The use of molecular typing methods is essential for determining the origin of the strains, their clonal relations, and also in epidemiological investigations. The purpose of this study was to determine the prevalence of antibiotic resistant S. aureus isolates and using spa, agr, and SCCmec typing to determine the dominant types in Iran. Material and Method. Fifty isolates of S. aureus were collected from January to May 2010. S. aureus identification was performed by biochemical tests. Disk diffusion method was employed to assess the sensitivity of S. aureus strains to antibiotics and then genetic analysis of bacteria was performed using SCCmec, agr, and spa typing. Results. S. aureus resistance to tetracycline, cefoxitin, clindamycin, ciprofloxacin, gentamicin, Cot: cotrimoxazole, levofloxacin, rifampin, and vancomycin were found to be 36%, 18%, 12%, 12%, 22%, 6%, 6%, and 0%, respectively. The results of this study showed that 16% of the isolates were resistant to methicillin (MRSA) and the majority of isolates were SSC mec type IV. In addition spa and agr typing revealed agr typeI and spa type t7688 to be the most predominant. Conclusion. In this study, spa typing showed 100% reliability and the t7688 spa type had a frequency of 26% compared to the frequency of 0.0% in the Ridom SpaServer. The frequency of t304 spa type was higher than the global average.
authors.affiliation: Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Seyed Asghar
authors.initials: SA
authors.lastname: Havaei
authors.affiliation: Department of Microbiology, Faculty of Bioscience, Islamic Azad University, Falavarjan Branch, Isfahan, Iran.
authors.forename: Fahimeh
authors.initials: F
authors.lastname: Ghanbari
authors.affiliation: Department of Molecular and Cell Biochemistry, Faculty of Bioscience, Falavarjan Branch, Islamic Azad University, Isfahan, Iran.
authors.forename: Ali Asghar
authors.initials: AA
authors.lastname: Rastegari
authors.affiliation: Department of Pathobiology and Anatomy, School of Medicine, North Khorasan University of Medical Science, Bojnord, Iran.
authors.forename: Amir
authors.identifier: 0000-0002-2532-8198
authors.initials: A
authors.lastname: Azimian
authors.affiliation: Department of Medical Bacteriology and Virology, Ghaem Hospital, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Farzad
authors.initials: F
authors.lastname: Khademi
authors.affiliation: Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Nafiseh
authors.initials: N
authors.lastname: Hosseini
authors.affiliation: Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Amirmorteza
authors.initials: A
authors.lastname: Ebrahimzadeh Namvar
authors.affiliation: Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Vaez
authors.affiliation: Department of Biology, Faculty of Sciences, Shiraz University, Shiraz, Iran.
authors.forename: Seyed Mehdi
authors.initials: SM
authors.lastname: Havaei
authors.affiliation: Department of Microbiology, School of Medicine, Ahvaz University of Medical Sciences, Ahvaz, Iran.
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Shahin