title: New ent-kaurane diterpenoid dimer from Pulicaria inuloides.
abstract: A new naturally occurring ent-kaurane diterpenoid dimer, 15β, 15'β-oxybis (ent-kaur-16-en-19-oic acid) (1) along with six known compounds, 15β-hydroxy-ent-kaur-16-en-19-oic acid (2), 15β-hydroxy-ent-kaur-16-en-19-oate-β-d-glucopyranoside (3), 6-hydroxykaempferol-3, 7-dimethyl ether (4), quercetagetin 3, 7, 3'-trimethyl ether (5), β-sitosterol (6) and β-sitosterol glucoside (daucosterol) (7) were isolated from the aerial parts of Pulicaria inuloides DC. Compounds 2-5 were isolated for the first time from genus Pulicaria. The structures of compounds 1-7 were established on the basis of extensive 1D and 2D NMR spectroscopic techniques in combination with ESI-MS. The antimicrobial activity of the isolated compounds was evaluated against Staphylococcus aureus, Escherichia coli and Candida albicans. Sulphorhodamine B cytotoxic assay against HepG2 (liver cancer) cell line and ABTS antioxidant assay were carried out.
authors.affiliation: a Faculty of Pharmacy, Department of Pharmacognosy , Mansoura University , Mansoura , Egypt.
authors.forename: Amal A
authors.initials: AA
authors.lastname: Galala
authors.affiliation: a Faculty of Pharmacy, Department of Pharmacognosy , Mansoura University , Mansoura , Egypt.
authors.forename: Amal
authors.initials: A
authors.lastname: Sallam
authors.affiliation: a Faculty of Pharmacy, Department of Pharmacognosy , Mansoura University , Mansoura , Egypt.|b Department of Pharmacognosy and Pharmaceutical Chemistry, College of Pharmacy , Taibah University , Al madinah Almonwwarah , Saudi Arabia.
authors.forename: Osama B
authors.initials: OB
authors.lastname: Abdel-Halim
authors.affiliation: a Faculty of Pharmacy, Department of Pharmacognosy , Mansoura University , Mansoura , Egypt.
authors.forename: Sahar R
authors.initials: SR
authors.lastname: Gedara
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D045786:Diterpenes, Kaurane / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D056945:Hep G2 Cells; D006801:Humans; D035261:Plant Components, Aerial / Q000737:chemistry; D052587:Pulicaria / Q000737:chemistry*
chemical_list: D000890:Anti-Infective Agents; D000972:Antineoplastic Agents, Phytogenic; D045786:Diterpenes, Kaurane
keywords: Asteraceae; Pulicaria inuloides; antimicrobial; antioxidant; cytotoxicity; ent-kaurane