title: Anti-proliferative Effect of Physcion on Human Gastric Cell Line via Inducing ROS-Dependent Apoptosis.
abstract: In this study, the anti-proliferative effect of physcion, an anthraquinone derivative isolated and characterized from both terrestrial and marine sources, against human gastric cancer SGC-7901 cells was investigated and the underlying mechanisms were explored. Physcion reduced SGC-7901 cell viability in a dose- and time-dependent manner, as demonstrated by MTT assay. It triggered the mitochondrial/caspase apoptotic pathway indicated by loss of mitochondrial membrane potential and cytochrome c release. Moreover, physcion induced a sustained activation of the phosphorylation of AMPK, and compound C (an inhibitor of AMPK) significantly reversed physcion-induced apoptosis in SGC-7901 cells. In addition, physcion provoked the generation of reactive oxygen species (ROS) in SGC-7901 cells, while the antioxidant N-acetyl cysteine almost completely blocked physcion-induced AMPK activation and apoptosis. Taken together, these findings suggest that physcion induces apoptosis through a ROS/AMPK-dependent mitochondrial pathway.
authors.affiliation: Department of Pharmacology, School of Medicine, Luzhou Medical College, Luzhou, 646000, Sichuan, China.
authors.forename: Yuxia
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: Department of Gastrointestinal Surgery, Affiliated Hospital of Luzhou Medical College, Luzhou, 646000, Sichuan, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Ren
authors.affiliation: Department of Gastroenterology, Affiliated Hospital of Luzhou Medical College, Luzhou, 646000, Sichuan, China.
authors.forename: Zhongqiong
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Gastrointestinal Surgery, Affiliated Hospital of Luzhou Medical College, Luzhou, 646000, Sichuan, China.
authors.forename: Zhengchuan
authors.initials: Z
authors.lastname: Hu
authors.affiliation: Department of Gastrointestinal Surgery, Affiliated Hospital of Luzhou Medical College, Luzhou, 646000, Sichuan, China. dryejiangzhou@163.com.
authors.forename: Yejiang
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D000111:Acetylcysteine / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D045304:Cytochromes c / Q000378:metabolism; D004642:Emodin / Q000031:analogs & derivatives* / Q000633:toxicity; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D010766:Phosphorylation / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology
chemical_list: D017382:Reactive Oxygen Species; D045304:Cytochromes c; D055372:AMP-Activated Protein Kinases; D053148:Caspase 3; C008905:physcione; D004642:Emodin; D000111:Acetylcysteine
keywords: AMPK; Apoptosis; Gastric cancer; ROS