title: Hsp27 gene in Drosophila ananassae subgroup was split by a recently acquired intron.
abstract: In Drosophila, heat shock protein 27 (Hsp27) is a critical single-copy intron-free nuclear gene involved in the defense response against fungi and bacteria, and is a regulator of adult lifespan. In the present study, 33 homologous Hsp27 nucleotide sequences from different Drosophila species were amplified by PCR and reverse transcription PCR, and the phylogenetic relationships were analysed using neighbour-joining, maximum-likelihood and Bayesian methods. The phylogenetic topologies from analysis with different algorithms were similar, suggesting that the Hsp27 gene was split by a recently acquired intron during the evolution of the Drosophila ananassae subgroup.
authors.affiliation: Hubei Collaborative Innovation Center for Green Transformation of Bioresources, College of Life Science, Hubei University, Wuhan 430062, People's Republic of China.Zengqit@hubu.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Han
authors.initials: H
authors.lastname: Kang
authors.forename: Shan
authors.initials: S
authors.lastname: Jin
authors.forename: Qing Tao
authors.initials: QT
authors.lastname: Zeng
authors.forename: Yong
authors.initials: Y
authors.lastname: Yang
mesh_terms: D000465:Algorithms; D000818:Animals; D001483:Base Sequence; D001499:Bayes Theorem; D005075:Biological Evolution; D002467:Cell Nucleus / Q000235:genetics; D004330:Drosophila / Q000145:classification / Q000235:genetics*; D029721:Drosophila Proteins / Q000737:chemistry / Q000235:genetics*; D005091:Exons; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics*; D007438:Introns; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D012984:Software; D013045:Species Specificity
chemical_list: D029721:Drosophila Proteins; D006360:Heat-Shock Proteins; C087442:Hsp26 protein, Drosophila