title: Vitamin D3 analog maxacalcitol (OCT) induces hCAP-18/LL-37 production in human oral epithelial cells.
abstract: Maxacalcitol (22-oxacalcitriol: OCT) is a synthetic vitamin D3 analog with a limited calcemic effect. In this study, we investigated whether OCT increases the production of LL-37/CAP-18, a human cathelicidin antimicrobial peptide, in human gingival/oral epithelial cells. A human gingival epithelial cell line (Ca9-22) and human oral epithelial cell lines (HSC-2, HSC-3, and HSC-4) exhibited the enhanced expression of LL-37 mRNA upon stimulation with OCT as well as active metabolites of vitamins D3 and D2. Among the human epithelial cell lines, Ca9-22 exhibited the strongest response to these vitamin D-related compounds. OCT induced the higher production of CAP-18 (ng/mL order) until 6 days time-dependently in Ca9-22 cells in culture. The periodontal pathogen Porphyromonas gingivalis was killed by treatment with the LL-37 peptide. These findings suggest that OCT induces the production of hCAP-18/LL-37 in a manner similar to that induced by the active metabolite of vitamin D3.
authors.affiliation: Division of Oral Microbiology, Tohoku University Graduate School of Dentistry.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tada
authors.forename: Takamitsu
authors.initials: T
authors.lastname: Shimizu
authors.forename: Isao
authors.initials: I
authors.lastname: Nagaoka
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Takada
mesh_terms: D023181:Antimicrobial Cationic Peptides / Q000096:biosynthesis* / Q000235:genetics; D002117:Calcitriol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D009061:Mouth Mucosa / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D054804:Cathelicidins
chemical_list: D023181:Antimicrobial Cationic Peptides; D012333:RNA, Messenger; D002117:Calcitriol; C051883:maxacalcitol; D054804:Cathelicidins