title: Novel class of collector in electrospinning device for the fabrication of 3D nanofibrous structure for large defect load-bearing tissue engineering application.
abstract: Adequate porosity, appropriate pore size, and 3D-thick shape are crucial parameters in the design of scaffolds, as they should provide the right space for cell adhesion, spreading, migration, and growth. In this work, a novel design for fabricating a 3D nanostructured scaffold by electrospinning was taken into account. Helical spring-shaped collector was purposely designed and used for electrospinning PCL fibers. Improved morphological properties and more uniform diameter distribution of collected nanofibers on the turns of helical spring-shaped collector are confirmed by SEM analysis. SEM images elaboration showed 3D pores with average diameter of 4 and 5.5 micrometer in x-y plane and z-direction, respectively. Prepared 3D scaffold possessed 99.98% porosity which led to the increased water uptake behavior in PBS at 37°C up to 10 days, and higher degradation rate compared to 2D flat structure. Uniaxial compression test on 3D scaffolds revealed an elastic modulus of 7 MPa and a stiffness of 102MPa, together with very low hysteresis area and residual strain. In vitro cytocompatibility test with MG-63 osteoblast-like cells using AlamarBlue™colorimetric assay, indicated a continuous increase in cell viability for the 3D structure over the test duration. SEM observation showed enhanced cells spreading and diffusion into the underneath layers for 3D scaffold. Accelerated calcium deposition in 3D substrate was confirmed by EDX analysis. Obtained morphological, physical, and mechanical properties together with in vitro cytocompatibility results, suggest this novel technique as a proper method for the fabrication of 3D nanofibrous scaffolds for the regeneration of critical-size load bearing defects. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1535-1548, 2017.
authors.affiliation: Department of Polymer Engineering and Color Technology, Amirkabir University of Technology (Tehran Polytechnic), 424 Hafez Avenue, Tehran, Iran.|Department of Chemistry, Materials and Chemical Engineering ''G. Natta'', Politecnico Di Milano, P.Zza Leonardo Da Vinci 32, Milan, 20133, Italy.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Hejazi
authors.affiliation: Department of Polymer Engineering and Color Technology, Amirkabir University of Technology (Tehran Polytechnic), 424 Hafez Avenue, Tehran, Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Mirzadeh
authors.affiliation: Department of Chemistry, Materials and Chemical Engineering ''G. Natta'', Politecnico Di Milano, P.Zza Leonardo Da Vinci 32, Milan, 20133, Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Contessi
authors.affiliation: Department of Chemistry, Materials and Chemical Engineering ''G. Natta'', Politecnico Di Milano, P.Zza Leonardo Da Vinci 32, Milan, 20133, Italy.
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Tanzi
authors.affiliation: Department of Chemistry, Materials and Chemical Engineering ''G. Natta'', Politecnico Di Milano, P.Zza Leonardo Da Vinci 32, Milan, 20133, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Faré
mesh_terms: D045744:Cell Line, Tumor; D006801:Humans; D008422:Materials Testing; D057139:Nanofibers / Q000737:chemistry*; D010006:Osteoblasts / Q000378:metabolism*; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
keywords: 3D nanofibrous scaffold; helical spring-shaped collector; in vitro cytocompatibility; mechanical properties; polycaprolactone; unconventional electrospinning