title: Broad and Temperature Independent Replication Potential of Filoviruses on Cells Derived From Old and New World Bat Species.
abstract: Filoviruses are strongly associated with several species of bats as their natural reservoirs. In this study, we determined the replication potential of all filovirus species: Marburg marburgvirus, Taï Forest ebolavirus, Reston ebolavirus, Sudan ebolavirus, Zaire ebolavirus, and Bundibugyo ebolavirus. Filovirus replication was supported by all cell lines derived from 6 Old and New World bat species: the hammer-headed fruit bat, Buettikofer's epauletted fruit bat, the Egyptian fruit bat, the Jamaican fruit bat, the Mexican free-tailed bat and the big brown bat. In addition, we showed that Marburg virus Angola and Ebola virus Makona-WPGC07 efficiently replicated at 37°C, 37°-41°C, or 41°C, contrary to the hypothesis that temporal elevation in temperature due to flight affects filovirus replication in bats.
authors.affiliation: Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, Montana.
authors.forename: Megan R
authors.initials: MR
authors.lastname: Miller
authors.affiliation: Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, Montana.
authors.forename: Rebekah J
authors.initials: RJ
authors.lastname: McMinn
authors.affiliation: Department of Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada.
authors.forename: Vikram
authors.initials: V
authors.lastname: Misra
authors.affiliation: Arthropod-borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins.
authors.forename: Tony
authors.initials: T
authors.lastname: Schountz
authors.affiliation: University of Bonn Medical Centre, Institute of Virology.
authors.forename: Marcel A
authors.initials: MA
authors.lastname: Müller
authors.affiliation: Biosafety Level 4 Laboratory, Centre for Biological Threats and Special Pathogens, Robert Koch Institute, Berlin, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Kurth
authors.affiliation: Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, Montana.
authors.forename: Vincent J
authors.initials: VJ
authors.lastname: Munster
mesh_terms: D000818:Animals; D002460:Cell Line; D002685:Chiroptera / Q000821:virology*; D004197:Disease Reservoirs / Q000821:virology*; D029043:Ebolavirus / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology; D016563:Filoviridae / Q000302:isolation & purification* / Q000502:physiology; D018702:Filoviridae Infections / Q000821:virology*; D019142:Hemorrhagic Fever, Ebola / Q000821:virology*; D006801:Humans; D008379:Marburg Virus Disease / Q000821:virology*; D029024:Marburgvirus / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology; D013696:Temperature; D014779:Virus Replication
keywords: Ebola virus; Marburg virus; bats; filovirus; replication; temperature