title: B7-H3 protein expression in a murine model of osteosarcoma.
abstract: Osteosarcoma is an aggressive type of bone tumor that commonly occurs in pediatric age groups. The complete molecular mechanisms behind osteosarcoma formation and progression require elucidation. B7-H3 is a protein of the B7 family that acts as a co-stimulatory molecule with a significant role in adaptive immune responses. The link between B7-H3 expression and its role in different types of cancer remains unclear. B7-H3 protein exhibits different functional roles inin vivoandin vitroconditions that remain controversial. In the present study, a murine model of osteosarcoma was successfully established using a modified protocol so as to easily obtain a low grade and metastatic form of osteosarcoma tissue without complication. Histological data showed that a less organized and highly proliferative mass of cells was observed in the osteosarcoma tissue. A higher expression level of B7-H3 protein was also observed at each advanced stage of osteosarcoma, which indicated the contributory role of the protein in the development of the primary and metastatic forms of osteosarcoma. Immunohistochemistry was performed, which showed that the overexpression of B7-H3 protein in the metastatic form of osteosarcoma may be associated with its migration and invasion.
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China; Department of Orthopedics, The Second People's Hospital of Huai'an, Huai'an, Jiangsu 223002, P.R. China.
authors.forename: Jia-Li
authors.initials: JL
authors.lastname: Zhao
authors.affiliation: Central Laboratory, The First People's Hospital of Huai'an, Huai'an, Jiangsu 223300, P.R. China.
authors.forename: Feng-Li
authors.initials: FL
authors.lastname: Chen
authors.affiliation: Department of Orthopedics, The Second People's Hospital of Huai'an, Huai'an, Jiangsu 223002, P.R. China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Department of Orthopedics, The Second People's Hospital of Huai'an, Huai'an, Jiangsu 223002, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Pan
authors.affiliation: Department of Orthopedics, The Second People's Hospital of Huai'an, Huai'an, Jiangsu 223002, P.R. China.
authors.forename: Xin-Hong
authors.initials: XH
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, The Second People's Hospital of Huai'an, Huai'an, Jiangsu 223002, P.R. China.
authors.forename: Jin
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Orthopedics, The Second People's Hospital of Huai'an, Huai'an, Jiangsu 223002, P.R. China.
authors.forename: Shao-Xian
authors.initials: SX
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, The Second People's Hospital of Huai'an, Huai'an, Jiangsu 223002, P.R. China.
authors.forename: L I
authors.initials: LI
authors.lastname: Ni
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China.
authors.forename: Hui-Lin
authors.initials: HL
authors.lastname: Yang
keywords: B7-H3 protein; BALB/c strain; K7M2 cells; osteosarcoma