title: Rapid and Sensitive Chemiluminescent Enzyme Immunoassay for the Determination of Neomycin Residues in Milk.
abstract: Immunoassays greatly contribute to veterinary drug residue analysis. However, there are few reports on detecting neomycin residues by immunoassay. Here, a rapid and sensitive chemiluminescent enzyme immunoassay (CLIEA) was successfully developed for neomycin residue analysis. CLIEA demonstrated good cross-reactivity for neomycin, and the IC50 value was 2.4 ng/mL in buffer. The average recovery range was 88.5%-105.4% for spiked samples (10, 50, and 100 μg/kg), and the coefficient of variation was in the range of 7.5%-14.5%. The limit of detection of CLEIA was 9.4 μg/kg, and this method was compared with the liquid chromatography-tandem mass spectrometry method using naturally contaminated samples, producing a correlation coefficient of >0.95. We demonstrate a reliable CLIEA for the rapid screening of neomycin in milk.
authors.affiliation: Key Laboratory of Food Safety Risk Assessment, Ministry of Health, China National Center for Food Safety Risk Assessment, Beijing 100022, China.
authors.forename: Peng Jie
authors.initials: PJ
authors.lastname: Luo
authors.affiliation: Key Laboratory of Food Safety Risk Assessment, Ministry of Health, China National Center for Food Safety Risk Assessment, Beijing 100022, China.
authors.forename: Jian Bo
authors.initials: JB
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Food Safety Risk Assessment, Ministry of Health, China National Center for Food Safety Risk Assessment, Beijing 100022, China.
authors.forename: Hua Li
authors.initials: HL
authors.lastname: Wang
authors.affiliation: State Key Laboratory for infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Physiology, School of Basic Medical Sciences, Shenzhen University Health Sciences Center, Shenzhen 518060, Guangdong, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Wu
authors.affiliation: Key Laboratory of Food Safety Risk Assessment, Ministry of Health, China National Center for Food Safety Risk Assessment, Beijing 100022, China.
authors.forename: Yun Feng
authors.initials: YF
authors.lastname: Zhao
authors.affiliation: Department of Physiology, School of Basic Medical Sciences, Shenzhen University Health Sciences Center, Shenzhen 518060, Guangdong, China.
authors.forename: Xiao Mei
authors.initials: XM
authors.lastname: Wang
authors.affiliation: Key Laboratory of Food Safety Risk Assessment, Ministry of Health, China National Center for Food Safety Risk Assessment, Beijing 100022, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Food Safety Risk Assessment, Ministry of Health, China National Center for Food Safety Risk Assessment, Beijing 100022, China.
authors.forename: Ji Yue
authors.initials: JY
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Food Safety Risk Assessment, Ministry of Health, China National Center for Food Safety Risk Assessment, Beijing 100022, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Department of Physiology, School of Basic Medical Sciences, Shenzhen University Health Sciences Center, Shenzhen 518060, Guangdong, China.
authors.forename: Wen Xiao
authors.initials: WX
authors.lastname: Jiang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D004350:Drug Residues / Q000378:metabolism; D005506:Food Contamination / Q000032:analysis*; D007124:Immunoenzyme Techniques / Q000662:veterinary*; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000662:veterinary*; D008892:Milk / Q000737:chemistry*; D009355:Neomycin / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D009355:Neomycin