title: Cinnamaldehyde derivatives inhibit degranulation and inflammatory mediator production in rat basophilic leukemia cells.
abstract: Mast cells play a critical role in allergic diseases. Therefore, development of new therapeutic agents that suppresses the activation of mast cells may help prevent or treat allergic diseases. Here, we investigated the anti-allergic effects of 4-chloro-cinnamaldehyde and 4-trifluoro-cinnamaldehyde in RBL-2H3 cells. β-Hexosaminidase assays revealed that degranulation of RBL-2H3 cells was decreased following treatment with 60μM 4-chloro-cinnamaldehyde or 4-trifluoro-cinnamaldehyde. Moreover, quantitative real-time reverse transcription polymerase chain reaction showed that the relative expression levels of tumor necrosis factor-α, interleukin-4, and cyclooxygenase-2 mRNAs were decreased in RBL-2H3 cells treated with 4-chloro-cinnamaldehyde and 4-trifluoro-cinnamaldehyde in a concentration-dependent manner. Additionally, 4-chloro-cinnamaldehyde blocked the phosphorylation of MKKs and MAPKs. These data clearly suggested that 4-chloro-cinnamaldehyde and 4-trifluoro-cinnamaldehyde had inhibitory effects on the inflammatory responses of mast cells and may have potential as novel therapeutic agents for the prevention or treatment of allergic diseases.
authors.affiliation: Department of Biomedical Technology, Kangwon National University, Chuncheon, Republic of Korea. Electronic address: ajin0317@gmail.com.
authors.forename: Sejin
authors.initials: S
authors.lastname: Ahn
authors.affiliation: Department of Biomedical Technology, Kangwon National University, Chuncheon, Republic of Korea. Electronic address: hammer345@naver.com.
authors.forename: Eunhee
authors.initials: E
authors.lastname: Kim
authors.affiliation: Department of Bio-health Technology, Kangwon National University, Chuncheon, Republic of Korea. Electronic address: lky@kangwon.ac.kr.
authors.forename: Kooyeon
authors.initials: K
authors.lastname: Lee
authors.affiliation: Department of Biomedical Technology, Kangwon National University, Chuncheon, Republic of Korea; Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon, Republic of Korea. Electronic address: dclee@kangwon.ac.kr.
authors.forename: Deug-Chan
authors.initials: DC
authors.lastname: Lee
mesh_terms: D000171:Acrolein / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D015550:Cell Degranulation / Q000187:drug effects; D045744:Cell Line, Tumor; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D006967:Hypersensitivity / Q000188:drug therapy* / Q000276:immunology; D018836:Inflammation Mediators / Q000378:metabolism; D015847:Interleukin-4 / Q000235:genetics / Q000378:metabolism; D008407:Mast Cells / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: D018836:Inflammation Mediators; D014409:Tumor Necrosis Factor-alpha; D015847:Interleukin-4; D000171:Acrolein; D051546:Cyclooxygenase 2; D048049:Extracellular Signal-Regulated MAP Kinases; C012843:cinnamaldehyde
keywords: 4-Chloro-cinnamaldehyde; 4-Trifluoro-cinnamaldehyde; Degranulation; Mitogen-activated protein kinases