title: Dietary Supplementation with a Low Dose of Polyphenol-Rich Grape Pomace Extract Prevents Dextran Sulfate Sodium-Induced Colitis in Rats.
abstract: Evidence from several epidemiological and experimental studies points to a beneficial role of dietary polyphenols in inflammatory bowel disease. In this study, we investigate the protective effect of dietary supplementation with various amounts of a polyphenol-rich grape pomace extract (GPE) on the development of dextran sulfate sodium (DSS)-induced colitis in rats. Rats were fed 21 days on a semisynthetic diet enriched with GPE (0.1%, 0.5%, and 1%), and acute colitis was induced by DSS (40 g/L in the drinking water) administration during the last 7 days. The low GPE content in the diet (0.1%) attenuated clinical signs and colon shortening and limited DSS-induced histological lesions. GPE 0.1% also attenuated the DSS-induced increase in myeloperoxidase activity and improved superoxide dismutase activity. Higher amounts of GPE in the diet induced only weak and nonsignificant protective effects. These results suggest that consumption of a low amount of polyphenol-rich GPE helps protect against colitis development.
authors.affiliation: 1 Clermont Université, Université d'Auvergne , UNH, ECREIN, Laboratoire de Pharmacognosie et Phytothérapie, Clermont-Ferrand, France .|2 3inature Biosphère , Parc Naturopôle, Saint-Bonnet-de-Rochefort, France .
authors.forename: Ahlem
authors.initials: A
authors.lastname: Boussenna
authors.affiliation: 3 CHU Clermont-Ferrand , Service d'Anatomie et de Cytologie Pathologiques, Clermont-Ferrand, France .
authors.forename: Juliette
authors.initials: J
authors.lastname: Joubert-Zakeyh
authors.affiliation: 1 Clermont Université, Université d'Auvergne , UNH, ECREIN, Laboratoire de Pharmacognosie et Phytothérapie, Clermont-Ferrand, France .
authors.forename: Didier
authors.initials: D
authors.lastname: Fraisse
authors.affiliation: 4 CHU Clermont-Ferrand , Délégation Recherche Clinique & Innovation, Clermont-Ferrand, France .
authors.forename: Bruno
authors.initials: B
authors.lastname: Pereira
authors.affiliation: 5 Clermont Université , Université d'Auvergne, UNH, ECREIN, Laboratoire de Biochimie, Biologie Moléculaire et Nutrition, Clermont-Ferrand, France .
authors.forename: Marie-Paule
authors.initials: MP
authors.lastname: Vasson
authors.affiliation: 1 Clermont Université, Université d'Auvergne , UNH, ECREIN, Laboratoire de Pharmacognosie et Phytothérapie, Clermont-Ferrand, France .
authors.forename: Odile
authors.initials: O
authors.lastname: Texier
authors.affiliation: 1 Clermont Université, Université d'Auvergne , UNH, ECREIN, Laboratoire de Pharmacognosie et Phytothérapie, Clermont-Ferrand, France .
authors.forename: Catherine
authors.initials: C
authors.lastname: Felgines
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D003092:Colitis / Q000097:blood / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D016264:Dextran Sulfate; D019587:Dietary Supplements; D005638:Fruit / Q000737:chemistry; D007249:Inflammation / Q000097:blood / Q000517:prevention & control; D015212:Inflammatory Bowel Diseases / Q000097:blood / Q000473:pathology / Q000517:prevention & control; D008297:Male; D009195:Peroxidase / Q000097:blood; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D059808:Polyphenols / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000097:blood; D027843:Vitis / Q000737:chemistry*
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D010936:Plant Extracts; D059808:Polyphenols; D016264:Dextran Sulfate; D009195:Peroxidase; D013482:Superoxide Dismutase
keywords: colon; grape pomace extract; inflammation; polyphenols; rat