title: Photobiomodulatory effects of superpulsed 904nm laser therapy on bioenergetics status in burn wound healing.
abstract: Burn wounds exhibit impaired healing as the progression through the normal sequential stages of tissue repair gets hampered by epidermal barrier disruption, compromised blood circulation, abrogated defence mechanism, pathologic inflammation, and septicemia. Our earlier results reported that superpulsed 904nm LLLT enhanced healing and attenuated inflammatory response in burn wounds. The present study investigated the effect of superpulsed 904nm LLLT (200ns pulse width; 100Hz; 0.7mW mean output power; 0.4mW/cm(2) average irradiance) on biochemical and molecular markers pertaining to bioenergetics and redox homeostasis on full-thickness burn wounds in experimental rats. Results indicated that superpulsed laser irradiation for 7days post-wounding propelled the cellular milieu towards aerobic energy metabolism as evidenced by significantly enhanced activities of key energy regulatory enzymes viz. HK, PFK, CS and G6PD, whereas LDH showed reduced activity as compared to the non-irradiated controls. LLLT showed a significant increased CCO activity and ATP level. Moreover, LLLT also regulated redox homeostasis as evidenced by enhanced NADPH levels and decreased NADP/NADPH ratio. Western blot analysis demonstrated that LLLT produced an up-regulation of GLUT1, pAMPKα and down-regulation of glycogen synthase1 (GS1). Our findings suggest that superpulsed 904nm LLLT augments burn wound healing by enhancing intracellular energy contents through modulation of aerobic metabolism for maximum energy output. Bioenergetic activation and maintenance of redox homeostasis could be one of the noteworthy mechanisms responsible for the beneficial NIR photobiomodulatory effect mediated through superpulsed 904nm LLLT in burn wound healing.
authors.affiliation: Defence Institute of Physiology and Allied Sciences (DIPAS), DRDO, Lucknow Road, Timarpur, Delhi 110 054, India.
authors.forename: Anju
authors.initials: A
authors.lastname: Yadav
authors.affiliation: Defence Institute of Physiology and Allied Sciences (DIPAS), DRDO, Lucknow Road, Timarpur, Delhi 110 054, India. Electronic address: asheeshgupta2001@gmail.com.
authors.forename: Asheesh
authors.initials: A
authors.lastname: Gupta
authors.affiliation: Defence Institute of Physiology and Allied Sciences (DIPAS), DRDO, Lucknow Road, Timarpur, Delhi 110 054, India.
authors.forename: Gaurav K
authors.initials: GK
authors.lastname: Keshri
authors.affiliation: Defence Institute of Physiology and Allied Sciences (DIPAS), DRDO, Lucknow Road, Timarpur, Delhi 110 054, India.
authors.forename: Saurabh
authors.initials: S
authors.lastname: Verma
authors.affiliation: Defence Institute of Physiology and Allied Sciences (DIPAS), DRDO, Lucknow Road, Timarpur, Delhi 110 054, India.
authors.forename: Sanjeev K
authors.initials: SK
authors.lastname: Sharma
authors.affiliation: Defence Institute of Physiology and Allied Sciences (DIPAS), DRDO, Lucknow Road, Timarpur, Delhi 110 054, India.
authors.forename: Shashi Bala
authors.initials: SB
authors.lastname: Singh
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002056:Burns / Q000378:metabolism / Q000473:pathology / Q000532:radiotherapy*; D004734:Energy Metabolism; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D009249:NADP / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014945:Wound Healing
chemical_list: D009249:NADP; D000255:Adenosine Triphosphate
keywords: ATP; Bioenergetics; Burn wound; Citrate synthase; Cytochrome c oxidase; Superpulsed 904nm LLLT