title: Clinical application of a ligation-independent pathway of multiplex ligation-dependent probe amplification for the determination of quinolone susceptibility of Streptococcus pneumoniae.
abstract: We previously uncovered a ligation-independent pathway of multiplex ligation-dependent probe amplification (MLPA) through which products of MLPA could be amplified without both hybridization and ligation reactions. Here, we utilized this pathway to detect an antibiotic resistance mutation of quinolones in Streptococcus pneumoniae.
authors.affiliation: Department of Laboratory Medicine, Nagasaki University, Graduate School of Biomedical Sciences, Nagasaki, Japan. Electronic address: nuno@nagasaki-u.ac.jp.
authors.forename: Naoki
authors.initials: N
authors.lastname: Uno
authors.affiliation: Department of Laboratory Medicine, Nagasaki University, Graduate School of Biomedical Sciences, Nagasaki, Japan.
authors.forename: Nobuko
authors.initials: N
authors.lastname: Araki
authors.affiliation: Department of Laboratory Medicine, Nagasaki University, Graduate School of Biomedical Sciences, Nagasaki, Japan.
authors.forename: Norihito
authors.initials: N
authors.lastname: Kaku
authors.affiliation: Department of Laboratory Medicine, Nagasaki University, Graduate School of Biomedical Sciences, Nagasaki, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Kosai
authors.affiliation: Department of Laboratory Medicine, Nagasaki University, Graduate School of Biomedical Sciences, Nagasaki, Japan.
authors.forename: Hiroo
authors.initials: H
authors.lastname: Hasegawa
authors.affiliation: Department of Laboratory Medicine, Nagasaki University, Graduate School of Biomedical Sciences, Nagasaki, Japan.
authors.forename: Katsunori
authors.initials: K
authors.lastname: Yanagihara
mesh_terms: D027081:DNA Gyrase / Q000235:genetics*; D004252:DNA Mutational Analysis; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial; D060885:Multiplex Polymerase Chain Reaction; D015363:Quinolones / Q000494:pharmacology*; D013296:Streptococcus pneumoniae / Q000187:drug effects* / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D015363:Quinolones; D027081:DNA Gyrase
keywords: Multiplex ligation-dependent probe amplification; Quinolone resistance-determining regions; Streptococcus pneumoniae