title: NFAT5 genes are part of the osmotic regulatory system in Atlantic salmon (Salmo salar).
abstract: The anadromous Atlantic salmon utilizes both fresh and salt water (FW and SW) habitats during its life cycle. The parr-smolt transformation (PST) is an important developmental transition from a FW adapted juvenile parr to a SW adapted smolt. Physiological changes in osmoregulatory tissues, particularly the gill, are key in maintaining effective ion regulation during PST. Changes are initiated prior to SW exposure (preparative phase), and are completed when smolts enter the sea (activational phase) where osmotic stress may directly stimulate changes in gene expression. In this paper we identify 4 nuclear factor of activated T cells (NFAT5, an osmotic stress transcription factor) paralogues in Atlantic salmon, which showed strong homology in characterized functional domains with those identified in other vertebrates. Two of the identified paralogues (NFAT5b1 and NFAT5b2) showed increased expression following transfer from FW to SW. This effect was largest in parr that were maintained under short day photoperiod, and showed the highest increases in chloride ion levels in response to SW exposure. The results of this study suggest that NFAT5 is involved in the osmotic stress response of Atlantic salmon.
authors.affiliation: Institute of Biological and Environmental Sciences, University of Aberdeen, AB24 2TZ, UK.
authors.forename: Marlene
authors.initials: M
authors.lastname: Lorgen
authors.affiliation: Department of Arctic and Marine Biology, Faculty of BioSciences Fisheries & Economy, University of Tromsø, Norway.
authors.forename: Even H
authors.initials: EH
authors.lastname: Jorgensen
authors.affiliation: Zoological Society London, Institute of Zoology, London NW1 4RY, UK.
authors.forename: William C
authors.initials: WC
authors.lastname: Jordan
authors.affiliation: Institute of Biological and Environmental Sciences, University of Aberdeen, AB24 2TZ, UK. Electronic address: sam.martin@abdn.ac.uk.
authors.forename: Samuel A M
authors.initials: SA
authors.lastname: Martin
authors.affiliation: Department of Arctic and Marine Biology, Faculty of BioSciences Fisheries & Economy, University of Tromsø, Norway.
authors.forename: David G
authors.initials: DG
authors.lastname: Hazlerigg
mesh_terms: D000818:Animals; D017753:Ecosystem; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D050778:NFATC Transcription Factors / Q000235:genetics* / Q000378:metabolism; D064587:Osmoregulation / Q000235:genetics*; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020317:Salmo salar / Q000235:genetics / Q000502:physiology*; D017422:Sequence Analysis, DNA / Q000662:veterinary; D012965:Sodium Chloride / Q000378:metabolism*
chemical_list: D029941:Fish Proteins; D050778:NFATC Transcription Factors; D012333:RNA, Messenger; D012965:Sodium Chloride
keywords: Nuclear activated factor of T-cells; Salinity; Salmonid; Thyroid hormone; Whole genome duplication