title: Platelets: handle with care.
abstract: Platelets are delicate cells that require careful handling between collection, preparation and transfusion. This review addresses practical questions relating to platelet concentration, resting time after collection, total time and number of periods without agitation and temperature. The bags in which platelets are stored are made from gas-permeable plastic to allow sufficient oxygen for the platelets to maintain aerobic respiration. Manufacturers have assigned limits for platelet content and concentration, and these must not be exceeded. There is no strong evidence for or against the resting of platelets post-collection and pre-agitation, but platelets should not be over-wrapped during this period as this compromises gas exchange; a short rest period of up to 1 h may allow the separation of minor aggregates. It is necessary to transport platelet concentrates (e.g. from manufacturing site to hospital), but these periods without gas exchange must be limited to avoid excessive damage to the platelets. Current data support a total of 24 h of transportation per component but with no individual period lasting more than 8 h. Platelets need to be stored at 20-24 °C based on evidence that colder storage leads to irreversible changes on the platelet membrane, resulting in phagocytosis of the platelets following transfusion. Storage at warmer temperatures may lead to an increase in bacterial risk. On the basis of this review, the UK Guidelines for Blood Transfusion Services have been updated to ensure that platelets are handled in the most appropriate way to ensure that efficacious components are provided for patients.
authors.affiliation: Manufacturing Development, NHS Blood and Transplant, Watford, UK. Stephen.Thomas@nhsbt.nhs.uk.
authors.forename: S
authors.initials: S
authors.lastname: Thomas
mesh_terms: D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism*; D001793:Blood Preservation / Q000379:methods* / Q000592:standards; D006801:Humans; D010100:Oxygen / Q000378:metabolism*; D010101:Oxygen Consumption; D017410:Practice Guidelines as Topic; D013997:Time Factors; D006113:United Kingdom
chemical_list: D010100:Oxygen
keywords: agitation; platelets; quality; transport