title: Calcitonin-like peptide hormone (CT/DH) in the frontal ganglia as a feeding regulatory peptide of the silkworm, Bombyx mori.
abstract: The frontal ganglion (FrG) in insects contributes to the modulation of feeding behavior via the regulation of foregut contraction and other neural networks. Profiling the peptides of the FrG is important to understand endocrine regulation of feeding behavior in insects. High-resolution spiral matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) identified an ion peak, corresponding to calcitonin-like diuretic hormone 31 (CT/DH) in the FrG of silkworm Bombyx mori larvae. RT-PCR confirmed that CT/DH is expressed in the FrG, as are other peptide hormones, including allatoregulatory peptides. A feeding latency assay using synthetic CT/DH revealed that it increases the time to the initiation of feeding in a dose-dependent manner. These data indicate that CT/DH is a candidate regulatory peptide that modulates the feeding behavior of B. mori.
authors.affiliation: Department of Integrated Bioscience, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwanoha 5-1-5, Kashiwa City, Chiba, #277-8562, Japan; Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. Electronic address: shinjin@edu.k.u-tokyo.ac.jp.
authors.forename: Shinji
authors.initials: S
authors.lastname: Nagata
authors.affiliation: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan; Present address: College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang 310058, China.
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Nagasawa
mesh_terms: D000818:Animals; D012831:Bombyx / Q000254:growth & development / Q000502:physiology*; D002116:Calcitonin / Q000031:analogs & derivatives*; D055098:Endocrine Cells / Q000378:metabolism; D005247:Feeding Behavior / Q000187:drug effects* / Q000502:physiology; D005724:Ganglia / Q000378:metabolism*; D007301:Insect Hormones / Q000235:genetics / Q000378:metabolism*; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*; D007814:Larva / Q000187:drug effects / Q000254:growth & development / Q000502:physiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors
chemical_list: D007301:Insect Hormones; D019476:Insect Proteins; D002116:Calcitonin
keywords: Bombyx mori; CT/DH; Calcitonin-like peptide; Feeding behavior