title: Derivation of Genea016 human embryonic stem cell line.
abstract: The Genea016 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, through ICM outgrowth on inactivated human feeders. The line showed pluripotent cell morphology and genomic analysis verified a 46, XX karyotype and female Allele pattern through traditional karyotyping, CGH and STR analysis. Pluripotency of Genea016 was demonstrated with 77% of cells expressing Nanog, 95% Oct4, 53% Tra1-60 and 98% SSEA4, a PluriTest Pluripotency score of 28.4, Novelty score of 1.37 and Alkaline Phosphatase activity. The cell line was negative for Mycoplasma and any visible contamination.
authors.affiliation: Genea Biocells, Sydney, Australia.
authors.forename: Biljana
authors.initials: B
authors.lastname: Dumevska
authors.affiliation: Genea Biocells, Sydney, Australia.
authors.forename: Omar
authors.initials: O
authors.lastname: Chami
authors.affiliation: Genea Biocells, Sydney, Australia.
authors.forename: Robert
authors.initials: R
authors.lastname: McKernan
authors.affiliation: Genea Biocells, Sydney, Australia.
authors.forename: Divya
authors.initials: D
authors.lastname: Goel
authors.affiliation: Genea Biocells, Sydney, Australia.
authors.forename: Teija
authors.initials: T
authors.lastname: Peura
authors.affiliation: Genea Biocells, Sydney, Australia.
authors.forename: Uli
authors.initials: U
authors.lastname: Schmidt
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line / Q000166:cytology*; D048430:Cell Shape; D055028:Comparative Genomic Hybridization; D004247:DNA / Q000378:metabolism; D000066449:Human Embryonic Stem Cells / Q000166:cytology*; D006801:Humans; D007621:Karyotyping; D015203:Reproducibility of Results; D013194:Staining and Labeling
chemical_list: D004247:DNA; D000469:Alkaline Phosphatase