title: Determination of current reference viruses for serological study of swine influenza viruses after the introduction of pandemic 2009 H1N1 (pdmH1N1) in Thailand.
abstract: Since the introduction of pandemic H1N1 2009 virus (pdmH1N1) in pigs, the status of Thai swine influenza virus (SIV) has changed. The pdmH1N1 and its reassortant viruses have become the predominant strain circulating in the Thai swine population based on the surveillance data from 2012 to 2014. For this reason, the reference viruses for serological assays using the hemagglutination inhibition (HI) test needed to be updated. Six anti-sera against reference viruses from 2006 to 2009 (enH1N1-06, enH1N1-09, enH1N2-09, pdmH1N1-09, enH3N2-07 and enH3N2-09) were used for the HI test with four contemporary viruses (enH1N1-10, pdmH1N1-10, rH1N2 and rH3N2) and the selected reference viruses were tested with sera collected from the field to determine the current SIV status. The results showed that anti-sera of swH1N1-06 had the highest titers against enH1N1-10. Anti-sera of pdmH1N1-09 had the highest titers against pdmH1N1-10 and rH1N2, whereas, anti-sera of enH3N2-09 had the highest titers against rH3N2. The results demonstrated that enH1N1-06, pdmH1N1-09 and enH3N2-09 should be selected as reference viruses for contemporary serological studies and HI tests. The seroprevalence results from 410 samples revealed enH1N1 (37.79%), pdmH1N1 (37.32%) and H3N2 (35.86%), respectively. The present study indicated that pdmH1N1 was widespread and commonly found in the Thai pig population increasing the risk of novel reassortant viruses and should be added as a reference virus for HI test. SIV surveillance program and serological studies should be conducted for the benefits of SIV control and prevention as well as monitoring for zoonotic potential.
authors.affiliation: Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Henri-Dunant Rd., Bangkok 10330, Thailand.
authors.forename: Jirapat
authors.initials: J
authors.lastname: Arunorat
authors.affiliation: Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Henri-Dunant Rd., Bangkok 10330, Thailand.
authors.forename: Nataya
authors.initials: N
authors.lastname: Charoenvisal
authors.affiliation: Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Henri-Dunant Rd., Bangkok 10330, Thailand.
authors.forename: Yonlayong
authors.initials: Y
authors.lastname: Woonwong
authors.affiliation: Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Henri-Dunant Rd., Bangkok 10330, Thailand.
authors.forename: Roongtham
authors.initials: R
authors.lastname: Kedkovid
authors.affiliation: Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Henri-Dunant Rd., Bangkok 10330, Thailand; Center of Emerging and Re-emerging Infectious Diseases in Animals, Faculty of Veterinary Science, Chulalongkorn University, Henri-Dunant Rd., Bangkok 10330, Thailand. Electronic address: roongroje.t@chula.ac.th.
authors.forename: Roongroje
authors.initials: R
authors.lastname: Thanawongnuwech
mesh_terms: D000818:Animals; D006385:Hemagglutination Inhibition Tests / Q000379:methods / Q000592:standards; D009975:Orthomyxoviridae / Q000145:classification / Q000302:isolation & purification*; D009976:Orthomyxoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D012015:Reference Standards; D016036:Seroepidemiologic Studies; D012698:Serologic Tests / Q000379:methods* / Q000592:standards*; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology* / Q000821:virology*; D013785:Thailand / Q000453:epidemiology
keywords: Hemagglutination inhibition; Influenza; Pandemic H1N1 2009; Swine; Thailand