title: MALAT1 induces tongue cancer cells' EMT and inhibits apoptosis through Wnt/β-catenin signaling pathway.
abstract: BACKGROUND
  MALAT1 is recognized as an oncogenic lncRNA in various malignancies. However, its expression and function in oral tongue squamous cell carcinoma are still unknown. This study aims to investigate the expression and function of MALAT1 in TSCC tissues and cells.
  
  
  METHODS
  qPCR was performed to detect the expression of MALAT1. MALAT1 was suppressed and upregulated by plasmid transfection in TSCC cells, and then cell migration, invasion, EMT, and apoptosis were analyzed.
  
  
  RESULTS
  LncRNA MALAT1 was upregulated in TSCC tissues and correlated with cervical lymph node metastasis in TSCC patients. Moreover, MALAT1 induced cell migration, invasion, EMT, and inhibited apoptosis by modulating Wnt/β-catenin signaling pathway. Finally, inhibiting Wnt/β-catenin signaling pathway attenuated the effect of exogenous MALAT1.
  
  
  CONCLUSIONS
  In summary, upregulated MALAT1 in TSCC promoted EMT and inhibited cell apoptosis by modulating Wnt/β-catenin signaling pathway.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liang
authors.affiliation: Department of Oral and Maxillofacial Surgery, Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, China.
authors.forename: Lizhong
authors.initials: L
authors.lastname: Liang
authors.affiliation: Department of Oral and Maxillofacial Surgery, Stomatological Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Kexiong
authors.initials: K
authors.lastname: Ouyang
authors.affiliation: Department of Oral and Maxillofacial Surgery, The Affiliated Stomatological Hospital of Southern Medical University & Guangdong Provincial Stomatological Hospital, Guangzhou, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Pathology, Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, China.
authors.forename: Xianping
authors.initials: X
authors.lastname: Yi
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism*; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology*; D005786:Gene Expression Regulation; D006801:Humans; D062085:RNA, Long Noncoding / Q000378:metabolism*; D015398:Signal Transduction; D014062:Tongue Neoplasms / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation; D051153:Wnt Proteins / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism
chemical_list: C557041:MALAT1 long non-coding RNA, human; D062085:RNA, Long Noncoding; D051153:Wnt Proteins; D051176:beta Catenin
keywords: MALAT1; epithelial-to-mesenchymal transition; invasion; migration; tongue squamous cell carcinoma