title: Supplementation with antioxidant-rich extra virgin olive oil prevents hepatic oxidative stress and reduction of desaturation capacity in mice fed a high-fat diet: Effects on fatty acid composition in liver and extrahepatic tissues.
abstract: OBJECTIVE
  The aim of this study was to assess the effect of dietary supplementation with extra virgin olive oil (EVOO) in mice on the reduction of desaturase and antioxidant enzymatic activities in liver, concomitantly with long-chain polyunsaturated fatty acids (LCPUFA) profiles in liver and extrahepatic tissues induced by a high-fat diet (HFD).
  
  
  METHODS
  Male mice C57 BL/6 J were fed with a control diet (CD; 10% fat, 20% protein, 70% carbohydrates) or an HFD (60% fat, 20% protein, 20% carbohydrates) for 12 wk. Animals were supplemented with 100 mg/d EVOO with different antioxidant contents (EVOO I, II, and III).
  
  
  RESULTS
  After the intervention, blood and several tissues were analyzed. Dietary supplementation with EVOO with the highest antioxidant content and antioxidant capacity (EVOO III) significantly reduced fat accumulation in liver and the plasmatic metabolic alterations caused by HFD and produced a normalization of oxidative stress-related parameters, desaturase activities, and LCPUFA content in tissues.
  
  
  CONCLUSIONS
  Data suggest that dietary supplementation with EVOO III may prevent oxidative stress and reduction of biosynthesis and accretion of ω-3 LCPUFA in the liver of HFD-fed mice.
authors.affiliation: Lipid Center, Institute of Nutrition and Food Technology, University of Chile, Santiago, Chile.
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Rincón-Cervera
authors.affiliation: Lipid Center, Institute of Nutrition and Food Technology, University of Chile, Santiago, Chile; Nutrition Department, Faculty of Medicine, University of Chile, Santiago, Chile. Electronic address: Rvalenzuelab@med.uchile.cl.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Valenzuela
authors.affiliation: Nutrition Department, Faculty of Medicine, University of Chile, Santiago, Chile.
authors.forename: María Catalina
authors.initials: MC
authors.lastname: Hernandez-Rodas
authors.affiliation: Nutrition Department, Faculty of Medicine, University of Chile, Santiago, Chile.
authors.forename: Macarena
authors.initials: M
authors.lastname: Marambio
authors.affiliation: Medical Technology Department, Faculty of Medicine, University of Chile, Santiago, Chile.
authors.forename: Alejandra
authors.initials: A
authors.lastname: Espinosa
authors.affiliation: Nutrition Department, Faculty of Medicine, University of Chile, Santiago, Chile.
authors.forename: Susana
authors.initials: S
authors.lastname: Mayer
authors.affiliation: Faculty of Chemical Sciences and Pharmacy, Department of Food Science and Chemical Technology, University of Chile, Santiago, Chile.
authors.forename: Nalda
authors.initials: N
authors.lastname: Romero
authors.affiliation: Nutrition Department, Faculty of Medicine, University of Chile, Santiago, Chile.
authors.forename: Cynthia
authors.initials: C
authors.lastname: Barrera M Sc
authors.affiliation: Lipid Center, Institute of Nutrition and Food Technology, University of Chile, Santiago, Chile.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Valenzuela
authors.affiliation: Molecular and Clinical Pharmacology Program, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Santiago, Chile.
authors.forename: Luis A
authors.initials: LA
authors.lastname: Videla
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects*; D019587:Dietary Supplements; D044943:Fatty Acid Desaturases / Q000235:genetics / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000378:metabolism; D043371:Fatty Acids, Omega-6 / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D000069463:Olive Oil / Q000008:administration & dosage* / Q000737:chemistry; D018384:Oxidative Stress; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics / Q000378:metabolism; D014018:Tissue Distribution
chemical_list: D000975:Antioxidants; D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6; D000069463:Olive Oil; D012333:RNA, Messenger; C497388:Srebf1 protein, mouse; D051780:Sterol Regulatory Element Binding Protein 1; D044943:Fatty Acid Desaturases
keywords: Antioxidants; Extra virgin olive oil; Oxidative stress; Δ;-5/Δ;-6 desaturase activities; ω-3 long-chain polyunsaturated fatty acid