title: [Analysis of the Squamous Cell Carcinoma Antigen (SCCA) Which Has a Significant Difference in Values between CLIA and FEIA--Case Report of Extra Glycosylation in SCCA].
abstract: Squamous cell carcinoma antigen (SCCA) is a glycoprotein that belongs to the serine protease inhibitor family. Clinically, it has been utilized as a tumor marker for squamous cell carcinoma. In clinical laboratories, SCCA is measured by several immunoassays. Recently, a number of studies have been reported that there is a significant difference in values between the immunoassays, attributing to SCCA-immunoglobulin complex. We found a case with significant difference in the SCCA value between CLIA and FEIA. In this case, SCCA-Immunoglobulin complex was not confirmed by gel filtration analysis. Interestingly, 5 to 10 kDa slightly-high molecular weight SCCA compared to control was detected by immunoblotting assay. It may be suspected that the aberrant glycosyl modification of SCCA influenced the reactivity to immunoassays.
authors.forename: Kanae
authors.initials: K
authors.lastname: Maruyama
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Aoki
authors.forename: Motoko
authors.initials: M
authors.lastname: Yamanaka
authors.forename: Miyuki
authors.initials: M
authors.lastname: Ono
authors.forename: Taeko
authors.initials: T
authors.lastname: Hotta
authors.forename: Dongchon
authors.initials: D
authors.lastname: Kang
mesh_terms: D000369:Aged, 80 and over; D000951:Antigens, Neoplasm / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis; D002850:Chromatography, Gel; D005260:Female; D015200:Fluoroimmunoassay; D006031:Glycosylation; D006801:Humans; D008970:Molecular Weight; D015843:Serpins / Q000032:analysis*; D014594:Uterine Neoplasms / Q000737:chemistry*
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D015843:Serpins; C051072:squamous cell carcinoma-related antigen