title: Differentiation between MAMP Triggered Defenses in Arabidopsis thaliana.
abstract: A first line of defense against pathogen attack for both plants and animals involves the detection of microbe-associated molecular patterns (MAMPs), followed by the induction of a complex immune response. Plants, like animals, encode several receptors that recognize different MAMPs. While these receptors are thought to function largely redundantly, the physiological responses to different MAMPs can differ in detail. Responses to MAMP exposure evolve quantitatively in natural populations of Arabidopsis thaliana, perhaps in response to environment specific differences in microbial threat. Here, we sought to determine the extent to which the detection of two canonical MAMPs were evolving redundantly or distinctly within natural populations. Our results reveal negligible correlation in plant growth responses between the bacterial MAMPs EF-Tu and flagellin. Further investigation of the genetic bases of differences in seedling growth inhibition and validation of 11 candidate genes reveal substantial differences in the genetic loci that underlie variation in response to these two MAMPs. Our results indicate that natural variation in MAMP recognition is largely MAMP-specific, indicating an ability to differentially tailor responses to EF-Tu and flagellin in A. thaliana populations.
authors.affiliation: Department of Ecology and Evolution, University of Chicago, Chicago, Illinois, United States of America.
authors.forename: Madlen
authors.initials: M
authors.lastname: Vetter
authors.affiliation: Department of Ecology and Evolution, University of Chicago, Chicago, Illinois, United States of America.|Committee on Genetics, Genomics and Systems Biology, University of Chicago, Chicago, Illinois, United States of America.
authors.forename: Talia L
authors.initials: TL
authors.lastname: Karasov
authors.affiliation: Department of Ecology and Evolution, University of Chicago, Chicago, Illinois, United States of America.
authors.forename: Joy
authors.initials: J
authors.lastname: Bergelson
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D029681:Arabidopsis Proteins / Q000096:biosynthesis / Q000235:genetics; D001483:Base Sequence; D018744:DNA, Plant / Q000235:genetics; D005408:Flagellin / Q000276:immunology*; D000069452:Pathogen-Associated Molecular Pattern Molecules / Q000276:immunology*; D010444:Peptide Elongation Factor Tu / Q000276:immunology*; D010935:Plant Diseases / Q000276:immunology / Q000382:microbiology; D011494:Protein Kinases / Q000096:biosynthesis / Q000235:genetics; D044224:Pseudomonas syringae / Q000378:metabolism*; D036226:Seedlings / Q000235:genetics / Q000254:growth & development*; D017422:Sequence Analysis, DNA; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology
chemical_list: D029681:Arabidopsis Proteins; D018744:DNA, Plant; D000069452:Pathogen-Associated Molecular Pattern Molecules; D005408:Flagellin; D011494:Protein Kinases; C411853:FLS2 protein, Arabidopsis; D010444:Peptide Elongation Factor Tu