title: Main regulatory pathways, key genes and microRNAs involved in flower formation and development of moso bamboo (Phyllostachys edulis).
abstract: Moso bamboo is characterized by infrequent sexual reproduction and erratic flowering habit; however, the molecular biology of flower formation and development is not well studied in this species. We studied the molecular regulation mechanisms of moso bamboo development and flowering by selecting three key regulatory pathways: plant-pathogen interaction, plant hormone signal transduction and protein processing in endoplasmic reticulum at different stages of flowering in moso bamboo. We selected PheDof1, PheMADS14 and six microRNAs involved in the three pathways through KEGG pathway and cluster analysis. Subcellular localization, transcriptional activation, Western blotting, in situ hybridization and qRT-PCR were used to further investigate the expression patterns and regulatory roles of pivotal genes at different flower development stages. Differential expression patterns showed that PheDof1, PheMADS14 and six miRNAs may play vital regulatory roles in flower development and floral transition in moso bamboo. Our research paves way for further studies on metabolic regulatory networks and provides insight into the molecular regulation mechanisms of moso bamboo flowering and senescence.
authors.affiliation: Key Laboratory of Bamboo and Rattan Science and Technology of the State Forestry Administration, International Centre for Bamboo and Rattan, Beijing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ge
authors.affiliation: Key Laboratory of Bamboo and Rattan Science and Technology of the State Forestry Administration, International Centre for Bamboo and Rattan, Beijing, China.|China National Engineering Research Center for Information Technology in Agriculture, Beijing, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Bamboo and Rattan Science and Technology of the State Forestry Administration, International Centre for Bamboo and Rattan, Beijing, China.
authors.forename: Zhanchao
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Bamboo and Rattan Science and Technology of the State Forestry Administration, International Centre for Bamboo and Rattan, Beijing, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Hou
authors.affiliation: Key Laboratory of Bamboo and Rattan Science and Technology of the State Forestry Administration, International Centre for Bamboo and Rattan, Beijing, China.
authors.forename: Xueping
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Bamboo and Rattan Science and Technology of the State Forestry Administration, International Centre for Bamboo and Rattan, Beijing, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Gao
mesh_terms: D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D016000:Cluster Analysis; D035264:Flowers / Q000235:genetics* / Q000254:growth & development*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D017403:In Situ Hybridization; D053858:Metabolic Networks and Pathways / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D058977:Molecular Sequence Annotation; D010802:Phylogeny; D006109:Poaceae / Q000235:genetics* / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D014157:Transcription Factors; D015533:Transcriptional Activation
chemical_list: D029681:Arabidopsis Proteins; D035683:MicroRNAs; D014157:Transcription Factors
keywords: Dof; MADS-box; flower formation and development; microRNA; moso bamboo; regulatory pathway