title: Aspirin inhibits growth of ovarian cancer by upregulating caspase-3 and downregulating bcl-2.
abstract: The aim of the present study was to investigate the effect and mechanism of different concentrations of aspirin in inhibiting the ovarian cancer of p53N236S gene knock-in mice. In total, 28 male p53S mice, with an age range of 4-6 weeks and weight of 20-25 g were selected. The animals were transplanted with SKOV3 cells to establish subdermal human ovarian cancer. The mice were randomly divided into different groups according to the aspirin concentrations (mmol/l) used, i.e., 0, 1, 2 and 3. Subsequently, intraperitoneal injection was performed once every two days for 3 weeks. The tumor volume, lifetime, tumor cell proliferation inhibition rates, caspase-3 protein and bcl-2 protein expression of the four groups were analyzed and compared. Following aspirin treatment for 1, 2 and 3 weeks, the tumor volume of the 3 mmol/l aspirin group was significantly smaller than the other groups (P<0.05). The higher concentration of aspirin led to a smaller tumor size (P<0.05). The cell proliferation inhibition rate of the 3 mmol/l aspirin group was significantly larger than that of other groups (P<0.05). The relative expression level of caspase-3, bcl-2 protein of the 3 mmol/l aspirin group was significantly improved and reduced, respectively. In conclusion, aspirin can inhibit the growth of ovarian cancer of p53S rats due to its upregulation of the expression of caspase-3 protein and downregulation of the expression of bcl-2 protein.
authors.affiliation: Department of Gynaecology and Obstetrics, Xiangyan Central Hospital, Hubei University of Arts and Science, Xiangyang, Hubei 441021, P.R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Gynaecology and Obstetrics, Xiangyan Central Hospital, Hubei University of Arts and Science, Xiangyang, Hubei 441021, P.R. China.
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Mao
authors.affiliation: Department of Gynaecology and Obstetrics, Xiangyan Central Hospital, Hubei University of Arts and Science, Xiangyang, Hubei 441021, P.R. China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Qin
authors.affiliation: Department of Gynaecology and Obstetrics, Xiangyan Central Hospital, Hubei University of Arts and Science, Xiangyang, Hubei 441021, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhou
authors.affiliation: Department of Gynaecology and Obstetrics, Xiangyan Central Hospital, Hubei University of Arts and Science, Xiangyang, Hubei 441021, P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xing
authors.affiliation: Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China.
authors.forename: Fan
authors.initials: F
authors.lastname: Dong
authors.affiliation: Dongfang Hospital, The Second Clinical Medical College of Beijing University of Chinese Medicine (BUCM), Beijing 100078, P.R. China.
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Jiang
authors.affiliation: School of Life Sciences, Fudan Univesity, Shanghai 200433, P.R. China.
authors.forename: Wenhui
authors.initials: W
authors.lastname: Zhuang
keywords: aspirin; bcl-2 protein; caspase-3 protein; ovarian cancer; p53S mice