title: Heat-treated Escherichia coli as a high-capacity biosorbent for tungsten anions.
abstract: Adsorption performance in the biosorption of tungsten using Escherichia coli cells can be significantly improved by using cell suspensions that have been heat-treated at ⩽100°C. In the case of E. coli cells suspension heated at 100°C, the aqueous tungsten ions concentration rapidly decreased from 0.8mmol/L to practically zero within 1h. This biosorption time is much shorter than that of non-heat treated E. coli cells (7h). Furthermore, the adsorption saturation amount for cells heat-treated at 100°C was significantly increased up to 1.62mmol-W/g-E. coli compared to the unheated E. coli cells case (0.62mmol-W/g-E. coli). Determination of the surface potential and surface structure along with quantitative analyses of free amino acids of heat-treated E. coli cells were also carried out and revealed that heated cells have a high zeta potential and express a higher concentration of amino acids on the cell surface.
authors.affiliation: Department of Chemical Engineering, Hiroshima University, 1-4-1 Kagamiyama, Higashi-Hiroshima 739-8527, Japan. Electronic address: ogit@hiroshima-u.ac.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Ogi
authors.affiliation: Cutting Tool R&D Division, Kyocera Corporation, Kagoshima Sendai Plant 1810 Taki-cho, Satsumasendai, Kagoshima 895-0292, Japan.
authors.forename: Takahiko
authors.initials: T
authors.lastname: Makino
authors.affiliation: Department of Physics, Bandung Institute of Technology, Ganesha 10, Bandung 40132, West Java, Indonesia.
authors.forename: Ferry
authors.initials: F
authors.lastname: Iskandar
authors.affiliation: Hiroshima Prefectural Technology Research Institute, 3-10-32 Kagamiyama, Higashi-Hiroshima 739-0046, Japan.
authors.forename: Eishi
authors.initials: E
authors.lastname: Tanabe
authors.affiliation: Department of Chemical Engineering, Hiroshima University, 1-4-1 Kagamiyama, Higashi-Hiroshima 739-8527, Japan.
authors.forename: Kikuo
authors.initials: K
authors.lastname: Okuyama
mesh_terms: D000327:Adsorption; D004926:Escherichia coli / Q000737:chemistry*; D006361:Heating; D008855:Microscopy, Electron, Scanning; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D014414:Tungsten / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D014867:Water; D014414:Tungsten
keywords: Amino acid analysis; Biosorption; Recycling engineering; Tungsten; Zeta potential