title: Knowledge on DNA Success Rates to Optimize the DNA Analysis Process: From Crime Scene to Laboratory.
abstract: DNA analysis has become an essential intelligence tool in the criminal justice system for the identification of possible offenders. However, it appears that about half of the processed DNA samples contains too little DNA for analysis. This study looks at DNA success rates within 28 different categories of trace exhibits and relates the DNA concentration to the characteristics of the DNA profile. Data from 2260 analyzed crime samples show that cigarettes, bloodstains, and headwear have relatively high success rates. Cartridge cases, crowbars, and tie-wraps are on the other end of the spectrum. These objective data can assist forensics in their selection process.The DNA success probability shows a positive relation with the DNA concentration. This finding enables the laboratory to set an evidence-based threshold value in the DNA analysis process. For instance, 958 DNA extracts had a concentration value of 6 pg/μL or less. Only 46 of the 958 low-level extracts provided meaningful DNA profiling data.
authors.affiliation: Amsterdam University of Applied Sciences, - Forensic Science, Leeuwenburg, Weesperzijde 190, 1097 DZ, Amsterdam, Netherlands.
authors.forename: Anna A
authors.initials: AA
authors.lastname: Mapes
authors.affiliation: Netherlands Forensic Institute - Department of Science, Interdisciplinary Research, Statistics and Knowledge Management (WISK), Laan van Ypenburg 6, 2497 GB, The Hague, The Netherlands.|Forensic Biology, University of Amsterdam, 1012 WX, Amsterdam, Netherlands.
authors.forename: Ate D
authors.initials: AD
authors.lastname: Kloosterman
authors.affiliation: Netherlands Forensic Institute - Human Biological Traces (HBS), Laan van Ypenburg 6, 2497 GB, The Hague, The Netherlands.
authors.forename: Vincent
authors.initials: V
authors.lastname: van Marion
authors.affiliation: Amsterdam University of Applied Sciences, - Forensic Science, Leeuwenburg, Weesperzijde 190, 1097 DZ, Amsterdam, Netherlands.|Dutch Police Academy - Forensic Investigation, Arnhemseweg 348, 7334 AC, Apeldoorn, The Netherlands.
authors.forename: Christianne J
authors.initials: CJ
authors.lastname: de Poot
mesh_terms: D001801:Blood Stains; D003415:Crime; D057237:Criminals; D004247:DNA / Q000032:analysis*; D016172:DNA Fingerprinting; D006801:Humans
chemical_list: D004247:DNA
keywords: DNA success rates; DNA typing; forensic laboratory; forensic process; forensic science; scene of crime officer