title: Evaluation of the angiogenic potency of a novel exopolysaccharide produced by the MK1 bacterial strain.
abstract: Angiogenesis is an essential physiological step in wound healing and other regenerative processes. Here, we evaluated the angiogenic properties of an exopolysaccharide (EPS) secreted by MK1 (MK1-EPS), a novel bacterial strain isolated from Neungee mushrooms. MK1-EPS significantly increased human umbilical vein endothelial cell (HUVEC) proliferation, migration, and vascular tube formation. MK1-EPS enhanced the phosphorylation of extracellular signal-related kinase (ERK), c-Jun N-terminal kinase (JNK), and p38, which are mitogen-activated protein kinases. In addition, the expression of p21 and intercellular adhesion molecule 1 (ICAM1), and phosphorylation of signal transducer and activator of transcription 3 (STAT3), but not of protein kinase B (AKT), were increased. Specific inhibitors of p38 (SB203580), ERK (PD98059), and JNK (SP600125) inhibited MK1-EPS-induced HUVEC proliferation, tube formation, and cell migration, and partially attenuated MKI-EPS-induced expression of p21 and ICAM1, and STAT3 phosphorylation. After surgical implantation into rabbit calvarial bone defects, new blood vessel formation was significantly higher with MK1-EPS composite bone granules than with granules alone, and new bone formation increased significantly. Therefore, MK1-EPS induces angiogenesis and may have potential for use as a bone regeneration agent in bone tissue engineering applications.
authors.affiliation: Bonecell Biotech Inc., 77, Dunsan-dong, Seo-gu, Daejeon, 302-830, Korea.
authors.forename: Ji-Yun
authors.initials: JY
authors.lastname: Park
authors.affiliation: Bonecell Biotech Inc., 77, Dunsan-dong, Seo-gu, Daejeon, 302-830, Korea. kyzmo@wku.ac.kr.|Wonkwang Bone Regeneration Research Institute, Wonkwang University, Iksan, 570-749, Korea. kyzmo@wku.ac.kr.
authors.forename: Beom Su
authors.initials: BS
authors.lastname: Kim
authors.affiliation: Wonkwang Bone Regeneration Research Institute, Wonkwang University, Iksan, 570-749, Korea. omslee@wku.ac.kr.|Department of Oral and Maxillofacial and Surgery, College of Dentistry, Daejeon Dental Hospital, Wonkwang University, 77 Dunsan-ro, Seo-gu, Daejeon, 302-120, Korea. omslee@wku.ac.kr.
authors.forename: Jun
authors.initials: J
authors.lastname: Lee
mesh_terms: D000363:Agaricales; D043925:Angiogenesis Inducing Agents / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification / Q000494:pharmacology*; D011817:Rabbits
chemical_list: D043925:Angiogenesis Inducing Agents; D011135:Polysaccharides, Bacterial
keywords: Angiogenesis; Exopolysaccharide; Human umbilical vein endothelial cell; MK1 bacteria; Signal pathway