title: Implementation of a repeated fed-batch process for the production of chitin-glucan complex by Komagataella pastoris.
abstract: The yeast Komagataella pastoris was cultivated under different fed-batch strategies for the production of chitin-glucan complex (CGC), a co-polymer of chitin and β-glucan. The tested fed-batch strategies included DO-stat mode, predefined feeding profile and repeated fed-batch operation. Although high cell dry mass and high CGC production were obtained under the tested DO-stat strategy in a 94h cultivation (159 and 29g/L, respectively), the overall biomass and CGC productivities were low (41 and 7.4g/Lday, respectively). Cultivation with a predefined profile significantly improved both biomass and CGC volumetric productivity (87 and 10.8g/Lday, respectively). Hence, this strategy was used to implement a repeated fed-batch process comprising 7 consecutive cycles. A daily production of 119-126g/L of biomass with a CGC content of 11-16wt% was obtained, thus proving this cultivation strategy is adequate to reach a high CGC productivity that ranged between 11 and 18g/Lday. The process was stable and reproducible in terms of CGC productivity and polymer composition, making it a promising strategy for further process development.
authors.affiliation: UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal.
authors.forename: Inês
authors.initials: I
authors.lastname: Farinha
authors.affiliation: UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal. Electronic address: a4406@fct.unl.pt.
authors.forename: Filomena
authors.initials: F
authors.lastname: Freitas
authors.affiliation: UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal.
authors.forename: Maria A M
authors.initials: MA
authors.lastname: Reis
mesh_terms: D061249:Batch Cell Culture Techniques; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D001709:Biotechnology; D002686:Chitin / Q000031:analogs & derivatives / Q000096:biosynthesis* / Q000737:chemistry; D005285:Fermentation; D005936:Glucans / Q000096:biosynthesis* / Q000737:chemistry; D005990:Glycerol / Q000378:metabolism; D010843:Pichia / Q000378:metabolism; D004718:Saccharomycetales / Q000378:metabolism*
chemical_list: D005936:Glucans; D002686:Chitin; D005990:Glycerol
keywords: Chitin-glucan complex (CGC); DO-stat; Glycerol; High cell density cultivation; Komagataella pastoris; Repeated fed-batch