title: CD90 and CD24 Co-Expression Is Associated with Pancreatic Intraepithelial Neoplasias.
abstract: Thy-1 (CD90) has been shown to be a potential marker for several different types of cancer. However, reports on CD90 expression in pancreatic intraepithelial neoplasia (PanIN) lesions are still limited where PanINs are the most important precursor lesion of pancreatic ductal adenocarcinoma (PDAC). Herein, we investigate candidate markers for PanIN lesions by examining the distribution and trend of CD90 and CD24 expression as well as their co-expression in various stages of PanINs. Thirty cases of PanINs, which were confirmed histopathologically and clinically, were used to evaluate protein expression of CD90 and CD24 by immunofluoresence double staining. CD90 was found to be mainly expressed in stroma around lesion ducts while not observed in acini and islets in PanINs. CD90 also showed increased expression in PanIN III compared to PanIN III. CD24 was mainly present in the cytoplasm and membrane of pancreatic ductal epithelia, especially in the apical epithelium of the duct. CD24 had higher expression in PanIN III compared with PanIN IIIIII or PanIN III. CD90 was expressed around CD24 sites, but there was little overlap between cells that expressed each of these proteins. A correlation analysis showed that these two proteins have a moderate relationship with PanIN stages respectively. These results suggest that co-expression of CD90 and CD24 may have an important role in the development and progression of PanINs, which is also conducive to early detection and treatment of PDAC.
authors.affiliation: Department of Surgery, University of Michigan Medical Center, Ann Arbor, Michigan, 48109, United States of America.|Department of Toxicology, School of Public Health, Shenyang Medical College, Liaoning, 110034, China.
authors.forename: Xiucong
authors.initials: X
authors.lastname: Pei
authors.affiliation: Department of Surgery, University of Michigan Medical Center, Ann Arbor, Michigan, 48109, United States of America.
authors.forename: Jianhui
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Surgery, University of Michigan Medical Center, Ann Arbor, Michigan, 48109, United States of America.
authors.forename: Rui
authors.initials: R
authors.lastname: Yang
authors.affiliation: Department of Surgery, University of Michigan Medical Center, Ann Arbor, Michigan, 48109, United States of America.
authors.forename: Zhijing
authors.initials: Z
authors.lastname: Tan
authors.affiliation: Department of Surgery, University of Michigan Medical Center, Ann Arbor, Michigan, 48109, United States of America.
authors.forename: Mingrui
authors.initials: M
authors.lastname: An
authors.affiliation: Department of Pathology, University of Michigan School of Medicine, Ann Arbor, Michigan, 48109, United States of America.
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Shi
authors.affiliation: Department of Surgery, University of Michigan Medical Center, Ann Arbor, Michigan, 48109, United States of America.
authors.forename: David M
authors.initials: DM
authors.lastname: Lubman
mesh_terms: D051927:CD24 Antigen / Q000378:metabolism*; D002278:Carcinoma in Situ / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D017493:Leukocyte Common Antigens / Q000378:metabolism; D060787:Neoplasm Grading; D010190:Pancreatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D013194:Staining and Labeling; D018800:Thy-1 Antigens / Q000378:metabolism*
chemical_list: D051927:CD24 Antigen; D018800:Thy-1 Antigens; D017493:Leukocyte Common Antigens