title: [The effect of human PBMC on the growth of oral squamous cell carcinoma cell].
abstract: OBJECTIVE
  To explore the effect and mechanism of activated peripheral blood mononuclear cell (PBM) on the growth and apoptosis of oral squamous carcinoma cells in vitro.
  
  
  METHODS
  ELISA, RT-PCR, MTT and flow cytometry were used to detect cytokines secretion and oral squamous carcinoma cell growth and apoptosis affected by activated PBMCs with PHA. The data was analyzed using SPSS17.0 software pakage.
  
  
  RESULTS
  The cytokines secretion, mRNA expression of granzyme B and perforin, inhibition of growth and induction of apoptosis of oral squamous carcinoma cells treated by the activated PBMCs with PHA were significantly higher than the control groups (P<0.05).
  
  
  CONCLUSIONS
  The human PBMC can be activated effectively with the stimulations of PHA, which can significantly inhibit growth and induce apoptosis of oral squamous carcinoma cells in vitro.
authors.affiliation: Department of Clinical Laboratory, Baoshan Traditional Chinese and Western Medicine Hospital. Shanghai 201900, China. E-mail:sunyu0816@sina.com.
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Clinical Laboratory, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine. Shanghai 200011, China.
authors.forename: Zu-jun
authors.initials: ZJ
authors.lastname: Sun
mesh_terms: D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000503:physiopathology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000502:physiology*; D009062:Mouth Neoplasms / Q000503:physiopathology*