title: The curli biogenesis genes expression level is unassociated with Enterobacter cloacae hsp60 clusters and PFGE genotypes.
abstract: The objective of this study was to determine the correlation between Enterobacter cloacae complex subspecies and clusters involved in UTI infections and specific pulsotypes, and to assess the contribution of major curli biogenesis genes (csgD, csgA) expression level to pathogenesis of clusters and genotypes. Based on the PFGE analysis, 37 different profiles were observed among which 8 profiles were common types. Real time PCR of csgD and csgA genes of 50 E. cloacae complex in relation to PFGE and hsp60 genotypes showed that all the genetic clusters are not equally involved in pathogenesis of urinary tract infections. It was elucidated in this study that isolates with common PFGE genotypes belonged to identical hsp60 clusters, and the foremost clusters (VI, III, and V) mainly comprised within PFGE common types. In our study, no significant correlation was detected between the specific hsp60 clusters or PFGE genotypes and the expression level of csgD and csgA genes (P-value > 0.05). This is the first study describing that unequivalent contribution of E. cloacae genotypes and clusters in pathogenesis of UTI, is not owing to varied curli biogenesis expression potential. The PFGE genotyping showed more discriminatory power than hsp60 genotyping for epidemiological studies and source tracking purpose.
authors.affiliation: Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Majid
authors.initials: M
authors.lastname: Akbari
authors.affiliation: Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Electronic address: b.bakhshi@modares.ac.ir.
authors.forename: Bita
authors.initials: B
authors.lastname: Bakhshi
authors.affiliation: Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Shahin
authors.initials: S
authors.lastname: Najar-Peerayeh
authors.affiliation: Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Behmanesh
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis*; D018834:Chaperonin 60 / Q000096:biosynthesis*; D016000:Cluster Analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D016972:Enterobacter cloacae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D004756:Enterobacteriaceae Infections / Q000382:microbiology; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D058889:Molecular Typing; D014552:Urinary Tract Infections / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60; C077481:Crl protein, Bacteria
keywords: Curli; Enterobacter cloacae; PFGE; Real time-PCR; hsp60