title: Proteome analysis of the Mycobacterium tuberculosis Beijing B0/W148 cluster.
abstract: Beijing B0/W148, a "successful" clone of Mycobacterium tuberculosis, is widespread in the Russian Federation and some countries of the former Soviet Union. Here, we used label-free gel-LC-MS/MS shotgun proteomics to discover features of Beijing B0/W148 strains that could explain their success. Qualitative and quantitative proteome analyses of Beijing B0/W148 strains allowed us to identify 1,868 proteins, including 266 that were differentially abundant compared with the control strain H37Rv. To predict the biological effects of the observed differences in protein abundances, we performed Gene Ontology analysis together with analysis of protein-DNA interactions using a gene regulatory network. Our results demonstrate that Beijing B0/W148 strains have increased levels of enzymes responsible for long-chain fatty acid biosynthesis, along with a coincident decrease in the abundance of proteins responsible for their degradation. Together with high levels of HsaA (Rv3570c) protein, involved in steroid degradation, these findings provide a possible explanation for the increased transmissibility of Beijing B0/W148 strains and their survival in host macrophages. Among other, we confirmed a very low level of the SseA (Rv3283) protein in Beijing B0/W148 characteristic for all «modern» Beijing strains, which could lead to increased DNA oxidative damage, accumulation of mutations, and potentially facilitate the development of drug resistance.
authors.affiliation: Federal Research and Clinical Centre of Physical-Chemical Medicine, Moscow, Russian Federation.
authors.forename: Julia
authors.initials: J
authors.lastname: Bespyatykh
authors.affiliation: Federal Research and Clinical Centre of Physical-Chemical Medicine, Moscow, Russian Federation.
authors.forename: Egor
authors.initials: E
authors.lastname: Shitikov
authors.affiliation: Federal Research and Clinical Centre of Physical-Chemical Medicine, Moscow, Russian Federation.
authors.forename: Ivan
authors.initials: I
authors.lastname: Butenko
authors.affiliation: Federal Research and Clinical Centre of Physical-Chemical Medicine, Moscow, Russian Federation.|Moscow Institute of Physics and Technology, Dolgoprudny, Russia.
authors.forename: Ilya
authors.initials: I
authors.lastname: Altukhov
authors.affiliation: Federal Research and Clinical Centre of Physical-Chemical Medicine, Moscow, Russian Federation.|Moscow Institute of Physics and Technology, Dolgoprudny, Russia.
authors.forename: Dmitry
authors.initials: D
authors.lastname: Alexeev
authors.affiliation: St. Petersburg Pasteur Institute, St. Petersburg, Russian Federation.
authors.forename: Igor
authors.initials: I
authors.lastname: Mokrousov
authors.affiliation: Research Institute of Phtisiopulmonology, St. Petersburg, Russian Federation.
authors.forename: Marine
authors.initials: M
authors.lastname: Dogonadze
authors.affiliation: Research Institute of Phtisiopulmonology, St. Petersburg, Russian Federation.
authors.forename: Viacheslav
authors.initials: V
authors.lastname: Zhuravlev
authors.affiliation: Research Institute of Phtisiopulmonology, St. Petersburg, Russian Federation.
authors.forename: Peter
authors.initials: P
authors.lastname: Yablonsky
authors.affiliation: Federal Research and Clinical Centre of Physical-Chemical Medicine, Moscow, Russian Federation.
authors.forename: Elena
authors.initials: E
authors.lastname: Ilina
authors.affiliation: Federal Research and Clinical Centre of Physical-Chemical Medicine, Moscow, Russian Federation.|Moscow Institute of Physics and Technology, Dolgoprudny, Russia.
authors.forename: Vadim
authors.initials: V
authors.lastname: Govorun
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis*; D002853:Chromatography, Liquid; D063990:Gene Ontology; D053263:Gene Regulatory Networks; D009169:Mycobacterium tuberculosis / Q000737:chemistry*; D020543:Proteome / Q000032:analysis*; D053719:Tandem Mass Spectrometry; D037521:Virulence Factors / Q000032:analysis
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; D037521:Virulence Factors