title: Surface-Induced Hydrogelation for Fluorescence and Naked-Eye Detections of Enzyme Activity in Blood.
abstract: Fluorescence probes have been widely applied for the detection of important analytes with high sensitivity and specificity. However, they cannot be directly applied for the detection in samples with autofluorescence such as blood. Herein, we demonstrated a simple but effective method of surface-induced self-assembly/hydrogelation for fluorescence detection of an enzyme in biological fluids including blood and cell lysates. The method utilizes an attracting glass surface to induce self-assembly of an enzyme-generating fluorescent probe. After removing the upper solution, the fluorescence turn-on at the glass surface can therefore be used for the detection of enzyme activity. By judging the thickness and color depth of hydrogels at the surface of the glass plates, we could also estimate the enzyme activity by naked eyes. Our study not only expands the application of molecular self-assembly but also provides a useful method that can be applied for direct detection of enzyme activity in complex biological samples such as blood and cell lysates.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research and ‡Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, and Synergetic Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University , Tianjin 300071, People's Republic of China.
authors.forename: Tengyan
authors.initials: T
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research and ‡Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, and Synergetic Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University , Tianjin 300071, People's Republic of China.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Liang
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research and ‡Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, and Synergetic Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University , Tianjin 300071, People's Republic of China.
authors.forename: Shenglu
authors.initials: S
authors.lastname: Ji
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research and ‡Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, and Synergetic Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University , Tianjin 300071, People's Republic of China.
authors.forename: Dan
authors.initials: D
authors.lastname: Ding
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research and ‡Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, and Synergetic Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University , Tianjin 300071, People's Republic of China.
authors.forename: Deling
authors.initials: D
authors.lastname: Kong
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research and ‡Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, and Synergetic Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University , Tianjin 300071, People's Republic of China.
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research and ‡Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, and Synergetic Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University , Tianjin 300071, People's Republic of China.
authors.forename: Zhimou
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D002460:Cell Line; D004798:Enzymes / Q000097:blood*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D051379:Mice; D009574:Nitro Compounds / Q000737:chemistry; D010069:Oxadiazoles / Q000737:chemistry; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry; D013050:Spectrometry, Fluorescence
chemical_list: C000625942:4-chloro-7-nitro-1,2,3-benzoxadiazole; D004798:Enzymes; D005456:Fluorescent Dyes; D020100:Hydrogels; D009574:Nitro Compounds; D010069:Oxadiazoles; D010455:Peptides; D000469:Alkaline Phosphatase