title: Benefit of HSP90α intervention on ischemia-reperfusion injury of venous blood-congested flaps.
abstract: In order to decrease the incidence of flap necrosis after reconstructive surgeries, new approaches are required. In the present study, a model of venous congested flaps in rats was established to test the heat shock protein (HSP) 90α, 'F-5', protein as an intervention therapy to alleviate ischemia-reperfusion injury. A recombinant plasmid pET15b-F-5 carrying theHSP90αgene was constructed and the induced protein was purified from bacterial cell cultures. The rats in the study were divided into three different intervention groups: group A rats were treated with normal saline prior to flap establishment, group B rats were treated with HSP90α, 'F-5', protein prior to flap establishment, and group C rats were treated with the same 'F-5' protein after the surgical procedure. Additionally, the reperfusion time-points, ischemia for 6 or 8 h (5 rats each), were established in each group. After set periods of time, the flaps were observed for skin appearance, blood flow, survival rate and histological changes including neovascularization and re-epithelialization. The results showed that the flaps in the rats pre-treated with 'F-5' protein performed better than the flaps of rats in the other two groups: the blood flow was higher, flap survival rate was increased, inflammatory cell infiltration was decreased and angiogenesis increased, and new skin structure was better completed by the end of the experiment. The parameters examind were improved for all the groups when the ischemia time was 6 h instead of 8 h. In conclusion, HSP90α intervention prior to flap establishment was shown to be beneficial in the model of ischemia-reperfusion injury in venous-congested flaps.
authors.affiliation: Department of Plastic and Cosmetic Surgery, Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong 266003, P.R. China; Department of Breast Surgery, Qingdao Eighth People's Hospital, Qingdao, Shandong 266100, P.R. China.
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Hu
authors.affiliation: Department of Plastic and Cosmetic Surgery, Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong 266003, P.R. China.
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Chen
authors.affiliation: Department of Plastic and Cosmetic Surgery, Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong 266003, P.R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Plastic and Cosmetic Surgery, Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong 266003, P.R. China.
authors.forename: Xiang-Feng
authors.initials: XF
authors.lastname: Leng
authors.affiliation: Department of Breast Surgery, Qingdao Eighth People's Hospital, Qingdao, Shandong 266100, P.R. China.
authors.forename: Xiao-Jian
authors.initials: XJ
authors.lastname: Fan
authors.affiliation: Department of Otolaryngology, Qingdao Eighth People's Hospital, Qingdao, Shandong 266100, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
keywords: heat shock protein 90; ischemia-reperfusion injury; venous blood-congested flap; ‘F-5 gene’