title: Markers Linked to Wheat Stem Rust Resistance Gene Sr11 Effective to Puccinia graminis f. sp. tritici Race TKTTF.
abstract: Wheat stem rust, caused by Puccinia graminis f. sp. tritici, can cause severe yield losses on susceptible wheat varieties and cultivars. Although stem rust can be controlled by the use of genetic resistance, population dynamics of P. graminis f. sp. tritici can frequently lead to defeat of wheat stem rust resistance genes. P. graminis f. sp. tritici race TKTTF caused a severe epidemic in Ethiopia on Ug99-resistant 'Digalu' in 2013 and 2014. The gene Sr11 confers resistance to race TKTTF and is present in 'Gabo 56'. We identified seven single-nucleotide polymorphism (SNP) markers linked to Sr11 from a cross between Gabo 56 and 'Chinese Spring' exploiting a 90K Infinium iSelect Custom beadchip. Five SNP markers were validated on a 'Berkut'/'Scalavatis' population that segregated for Sr11, using KBioscience competitive allele-specific polymerase chain reaction (KASP) assays. Two of the SNP markers, KASP_6BL_IWB10724 and KASP_6BL_IWB72471, were predictive of Sr11 among wheat genetic stocks, cultivars, and breeding lines from North America, Ethiopia, and Pakistan. These markers can be utilized to select for Sr11 in wheat breeding and to detect the presence of Sr11 in uncharacterized germplasm.
authors.affiliation: First, twelfth, and thirteenth authors: Cereal Disease Laboratory, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), St. Paul, MN 55108; second author: Cereal Crops Research, USDA-ARS, Fargo, ND, 58102; third, twelfth, and thirteenth authors: Department of Plant Pathology, University of Minnesota, St. Paul 55108; fourth author: Small Grains and Potato Germplasm Research Unit, USDA-ARS, Aberdeen, ID 83210; fifth author: International Maize and Wheat Improvement Center (CIMMYT)-Ethiopia, Addis Ababa, Ethiopia; sixth author: Ethiopian Institute of Agricultural Research, Kulumsa Agricultural Research Center, Kulumsa, Ethiopia; seventh author: CIMMYT-Pakistan, Islamabad, Pakistan; eighth and ninth authors: Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman 59717; tenth author: Department of Plant Pathology, Kansas State University, Manhattan 66506; and eleventh author: Department of Crop and Soil Sciences, Washington State University, Pullman, 99164.
authors.forename: Jayaveeramuthu
authors.initials: J
authors.lastname: Nirmala
authors.affiliation: First, twelfth, and thirteenth authors: Cereal Disease Laboratory, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), St. Paul, MN 55108; second author: Cereal Crops Research, USDA-ARS, Fargo, ND, 58102; third, twelfth, and thirteenth authors: Department of Plant Pathology, University of Minnesota, St. Paul 55108; fourth author: Small Grains and Potato Germplasm Research Unit, USDA-ARS, Aberdeen, ID 83210; fifth author: International Maize and Wheat Improvement Center (CIMMYT)-Ethiopia, Addis Ababa, Ethiopia; sixth author: Ethiopian Institute of Agricultural Research, Kulumsa Agricultural Research Center, Kulumsa, Ethiopia; seventh author: CIMMYT-Pakistan, Islamabad, Pakistan; eighth and ninth authors: Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman 59717; tenth author: Department of Plant Pathology, Kansas State University, Manhattan 66506; and eleventh author: Department of Crop and Soil Sciences, Washington State University, Pullman, 99164.
authors.forename: Shiaoman
authors.initials: S
authors.lastname: Chao
authors.affiliation: First, twelfth, and thirteenth authors: Cereal Disease Laboratory, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), St. Paul, MN 55108; second author: Cereal Crops Research, USDA-ARS, Fargo, ND, 58102; third, twelfth, and thirteenth authors: Department of Plant Pathology, University of Minnesota, St. Paul 55108; fourth author: Small Grains and Potato Germplasm Research Unit, USDA-ARS, Aberdeen, ID 83210; fifth author: International Maize and Wheat Improvement Center (CIMMYT)-Ethiopia, Addis Ababa, Ethiopia; sixth author: Ethiopian Institute of Agricultural Research, Kulumsa Agricultural Research Center, Kulumsa, Ethiopia; seventh author: CIMMYT-Pakistan, Islamabad, Pakistan; eighth and ninth authors: Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman 59717; tenth author: Department of Plant Pathology, Kansas State University, Manhattan 66506; and eleventh author: Department of Crop and Soil Sciences, Washington State University, Pullman, 99164.
authors.forename: Pablo
authors.initials: P
authors.lastname: Olivera
authors.affiliation: First, twelfth, and thirteenth authors: Cereal Disease Laboratory, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), St. Paul, MN 55108; second author: Cereal Crops Research, USDA-ARS, Fargo, ND, 58102; third, twelfth, and thirteenth authors: Department of Plant Pathology, University of Minnesota, St. Paul 55108; fourth author: Small Grains and Potato Germplasm Research Unit, USDA-ARS, Aberdeen, ID 83210; fifth author: International Maize and Wheat Improvement Center (CIMMYT)-Ethiopia, Addis Ababa, Ethiopia; sixth author: Ethiopian Institute of Agricultural Research, Kulumsa Agricultural Research Center, Kulumsa, Ethiopia; seventh author: CIMMYT-Pakistan, Islamabad, Pakistan; eighth and ninth authors: Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman 59717; tenth author: Department of Plant Pathology, Kansas State University, Manhattan 66506; and eleventh author: Department of Crop and Soil Sciences, Washington State University, Pullman, 99164.
authors.forename: Ebrahiem M
authors.initials: EM
authors.lastname: Babiker
authors.affiliation: First, twelfth, and thirteenth authors: Cereal Disease Laboratory, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), St. Paul, MN 55108; second author: Cereal Crops Research, USDA-ARS, Fargo, ND, 58102; third, twelfth, and thirteenth authors: Department of Plant Pathology, University of Minnesota, St. Paul 55108; fourth author: Small Grains and Potato Germplasm Research Unit, USDA-ARS, Aberdeen, ID 83210; fifth author: International Maize and Wheat Improvement Center (CIMMYT)-Ethiopia, Addis Ababa, Ethiopia; sixth author: Ethiopian Institute of Agricultural Research, Kulumsa Agricultural Research Center, Kulumsa, Ethiopia; seventh author: CIMMYT-Pakistan, Islamabad, Pakistan; eighth and ninth authors: Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman 59717; tenth author: Department of Plant Pathology, Kansas State University, Manhattan 66506; and eleventh author: Department of Crop and Soil Sciences, Washington State University, Pullman, 99164.
authors.forename: Bekele
authors.initials: B
authors.lastname: Abeyo
authors.affiliation: First, twelfth, and thirteenth authors: Cereal Disease Laboratory, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), St. Paul, MN 55108; second author: Cereal Crops Research, USDA-ARS, Fargo, ND, 58102; third, twelfth, and thirteenth authors: Department of Plant Pathology, University of Minnesota, St. Paul 55108; fourth author: Small Grains and Potato Germplasm Research Unit, USDA-ARS, Aberdeen, ID 83210; fifth author: International Maize and Wheat Improvement Center (CIMMYT)-Ethiopia, Addis Ababa, Ethiopia; sixth author: Ethiopian Institute of Agricultural Research, Kulumsa Agricultural Research Center, Kulumsa, Ethiopia; seventh author: CIMMYT-Pakistan, Islamabad, Pakistan; eighth and ninth authors: Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman 59717; tenth author: Department of Plant Pathology, Kansas State University, Manhattan 66506; and eleventh author: Department of Crop and Soil Sciences, Washington State University, Pullman, 99164.
authors.forename: Zerihun
authors.initials: Z
authors.lastname: Tadesse
authors.affiliation: First, twelfth, and thirteenth authors: Cereal Disease Laboratory, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), St. Paul, MN 55108; second author: Cereal Crops Research, USDA-ARS, Fargo, ND, 58102; third, twelfth, and thirteenth authors: Department of Plant Pathology, University of Minnesota, St. Paul 55108; fourth author: Small Grains and Potato Germplasm Research Unit, USDA-ARS, Aberdeen, ID 83210; fifth author: International Maize and Wheat Improvement Center (CIMMYT)-Ethiopia, Addis Ababa, Ethiopia; sixth author: Ethiopian Institute of Agricultural Research, Kulumsa Agricultural Research Center, Kulumsa, Ethiopia; seventh author: CIMMYT-Pakistan, Islamabad, Pakistan; eighth and ninth authors: Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman 59717; tenth author: Department of Plant Pathology, Kansas State University, Manhattan 66506; and eleventh author: Department of Crop and Soil Sciences, Washington State University, Pullman, 99164.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Imtiaz
authors.affiliation: First, twelfth, and thirteenth authors: Cereal Disease Laboratory, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), St. Paul, MN 55108; second author: Cereal Crops Research, USDA-ARS, Fargo, ND, 58102; third, twelfth, and thirteenth authors: Department of Plant Pathology, University of Minnesota, St. Paul 55108; fourth author: Small Grains and Potato Germplasm Research Unit, USDA-ARS, Aberdeen, ID 83210; fifth author: International Maize and Wheat Improvement Center (CIMMYT)-Ethiopia, Addis Ababa, Ethiopia; sixth author: Ethiopian Institute of Agricultural Research, Kulumsa Agricultural Research Center, Kulumsa, Ethiopia; seventh author: CIMMYT-Pakistan, Islamabad, Pakistan; eighth and ninth authors: Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman 59717; tenth author: Department of Plant Pathology, Kansas State University, Manhattan 66506; and eleventh author: Department of Crop and Soil Sciences, Washington State University, Pullman, 99164.
authors.forename: Luther
authors.initials: L
authors.lastname: Talbert
authors.affiliation: First, twelfth, and thirteenth authors: Cereal Disease Laboratory, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), St. Paul, MN 55108; second author: Cereal Crops Research, USDA-ARS, Fargo, ND, 58102; third, twelfth, and thirteenth authors: Department of Plant Pathology, University of Minnesota, St. Paul 55108; fourth author: Small Grains and Potato Germplasm Research Unit, USDA-ARS, Aberdeen, ID 83210; fifth author: International Maize and Wheat Improvement Center (CIMMYT)-Ethiopia, Addis Ababa, Ethiopia; sixth author: Ethiopian Institute of Agricultural Research, Kulumsa Agricultural Research Center, Kulumsa, Ethiopia; seventh author: CIMMYT-Pakistan, Islamabad, Pakistan; eighth and ninth authors: Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman 59717; tenth author: Department of Plant Pathology, Kansas State University, Manhattan 66506; and eleventh author: Department of Crop and Soil Sciences, Washington State University, Pullman, 99164.
authors.forename: Nancy K
authors.initials: NK
authors.lastname: Blake
authors.affiliation: First, twelfth, and thirteenth authors: Cereal Disease Laboratory, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), St. Paul, MN 55108; second author: Cereal Crops Research, USDA-ARS, Fargo, ND, 58102; third, twelfth, and thirteenth authors: Department of Plant Pathology, University of Minnesota, St. Paul 55108; fourth author: Small Grains and Potato Germplasm Research Unit, USDA-ARS, Aberdeen, ID 83210; fifth author: International Maize and Wheat Improvement Center (CIMMYT)-Ethiopia, Addis Ababa, Ethiopia; sixth author: Ethiopian Institute of Agricultural Research, Kulumsa Agricultural Research Center, Kulumsa, Ethiopia; seventh author: CIMMYT-Pakistan, Islamabad, Pakistan; eighth and ninth authors: Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman 59717; tenth author: Department of Plant Pathology, Kansas State University, Manhattan 66506; and eleventh author: Department of Crop and Soil Sciences, Washington State University, Pullman, 99164.
authors.forename: Eduard
authors.initials: E
authors.lastname: Akhunov
authors.affiliation: First, twelfth, and thirteenth authors: Cereal Disease Laboratory, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), St. Paul, MN 55108; second author: Cereal Crops Research, USDA-ARS, Fargo, ND, 58102; third, twelfth, and thirteenth authors: Department of Plant Pathology, University of Minnesota, St. Paul 55108; fourth author: Small Grains and Potato Germplasm Research Unit, USDA-ARS, Aberdeen, ID 83210; fifth author: International Maize and Wheat Improvement Center (CIMMYT)-Ethiopia, Addis Ababa, Ethiopia; sixth author: Ethiopian Institute of Agricultural Research, Kulumsa Agricultural Research Center, Kulumsa, Ethiopia; seventh author: CIMMYT-Pakistan, Islamabad, Pakistan; eighth and ninth authors: Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman 59717; tenth author: Department of Plant Pathology, Kansas State University, Manhattan 66506; and eleventh author: Department of Crop and Soil Sciences, Washington State University, Pullman, 99164.
authors.forename: Michael O
authors.initials: MO
authors.lastname: Pumphrey
authors.affiliation: First, twelfth, and thirteenth authors: Cereal Disease Laboratory, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), St. Paul, MN 55108; second author: Cereal Crops Research, USDA-ARS, Fargo, ND, 58102; third, twelfth, and thirteenth authors: Department of Plant Pathology, University of Minnesota, St. Paul 55108; fourth author: Small Grains and Potato Germplasm Research Unit, USDA-ARS, Aberdeen, ID 83210; fifth author: International Maize and Wheat Improvement Center (CIMMYT)-Ethiopia, Addis Ababa, Ethiopia; sixth author: Ethiopian Institute of Agricultural Research, Kulumsa Agricultural Research Center, Kulumsa, Ethiopia; seventh author: CIMMYT-Pakistan, Islamabad, Pakistan; eighth and ninth authors: Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman 59717; tenth author: Department of Plant Pathology, Kansas State University, Manhattan 66506; and eleventh author: Department of Crop and Soil Sciences, Washington State University, Pullman, 99164.
authors.forename: Yue
authors.initials: Y
authors.lastname: Jin
authors.affiliation: First, twelfth, and thirteenth authors: Cereal Disease Laboratory, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), St. Paul, MN 55108; second author: Cereal Crops Research, USDA-ARS, Fargo, ND, 58102; third, twelfth, and thirteenth authors: Department of Plant Pathology, University of Minnesota, St. Paul 55108; fourth author: Small Grains and Potato Germplasm Research Unit, USDA-ARS, Aberdeen, ID 83210; fifth author: International Maize and Wheat Improvement Center (CIMMYT)-Ethiopia, Addis Ababa, Ethiopia; sixth author: Ethiopian Institute of Agricultural Research, Kulumsa Agricultural Research Center, Kulumsa, Ethiopia; seventh author: CIMMYT-Pakistan, Islamabad, Pakistan; eighth and ninth authors: Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman 59717; tenth author: Department of Plant Pathology, Kansas State University, Manhattan 66506; and eleventh author: Department of Crop and Soil Sciences, Washington State University, Pullman, 99164.
authors.forename: Matthew N
authors.initials: MN
authors.lastname: Rouse
mesh_terms: D000483:Alleles; D001487:Basidiomycota / Q000502:physiology*; D001947:Breeding; D060467:Disease Resistance / Q000235:genetics*; D005002:Ethiopia; D008040:Genetic Linkage; D005819:Genetic Markers / Q000235:genetics; D005838:Genotype; D009656:North America; D010154:Pakistan; D010641:Phenotype; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D018515:Plant Leaves / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D018547:Plant Stems / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D036226:Seedlings / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D014908:Triticum / Q000235:genetics* / Q000276:immunology / Q000382:microbiology
chemical_list: D005819:Genetic Markers