title: Identification of Neuregulin-2 as a novel stress granule component.
abstract: Stress Granules (SGs) are microscopically visible, phase dense aggregates of translationally stalled messenger ribonucleoprotein (mRNP) complexes formed in response to distinct stress conditions. It is generally considered that SG formation is induced to protect cells from conditions of stress. The precise constituents of SGs and the mechanism through which SGs are dynamically regulated in response to stress are not completely understood. Hence, it is important to identify proteins which regulate SG assembly and disassembly. In the present study, we report Neuregulin-2 (NRG2) as a novel component of SGs; furthermore, depletion of NRG2 potently inhibits SG formation. We also demonstrate that NRG2 specifically localizes to SGs under various stress conditions. Knockdown of NRG2 has no effect on stress-induced polysome disassembly, suggesting that the component does not influence early step of SG formation. It was also observed that reduced expression of NRG2 led to marginal increase in cell survival under arsenite-induced stress. [BMB Reports 2016; 49(8): 449-454].
authors.affiliation: Department of Cellular & Molecular Medicine, College of Medicine, Chosun University, Gwangju 61452, Korea.
authors.forename: Jin Ah
authors.initials: JA
authors.lastname: Kim
authors.affiliation: Department of Cellular & Molecular Medicine, College of Medicine, Chosun University, Gwangju 61452, Korea.
authors.forename: Aravinth Kumar
authors.initials: AK
authors.lastname: Jayabalan
authors.affiliation: Department of Cellular & Molecular Medicine, College of Medicine, Chosun University, Gwangju 61452, Korea.
authors.forename: Vinoth Kumar
authors.initials: VK
authors.lastname: Kothandan
authors.affiliation: Department of Cellular & Molecular Medicine, College of Medicine, Chosun University, Gwangju 61452, Korea.
authors.forename: Ramesh
authors.initials: R
authors.lastname: Mariappan
authors.affiliation: Department of Cell Biology, Microbiology, and Molecular Biology, College of Arts and Sciences, University of South Florida, Tampa, Florida 33620, USA.
authors.forename: Younghoon
authors.initials: Y
authors.lastname: Kee
authors.affiliation: Department of Cellular & Molecular Medicine, College of Medicine, Chosun University, Gwangju 61452, Korea.
authors.forename: Takbum
authors.initials: T
authors.lastname: Ohn
mesh_terms: D018053:Arsenites / Q000633:toxicity; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003594:Cytoplasmic Granules / Q000187:drug effects / Q000378:metabolism*; D017353:Gene Deletion; D055785:Gene Knockdown Techniques; D057809:HEK293 Cells; D006801:Humans; D009414:Nerve Growth Factors / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D011132:Polyribosomes / Q000187:drug effects / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D013312:Stress, Physiological / Q000187:drug effects
chemical_list: D018053:Arsenites; C106766:NRG2 protein, human; D009414:Nerve Growth Factors; D034741:RNA, Small Interfering; C015001:arsenite