title: An electrochemical sensor for detection of neurotransmitter-acetylcholine using metal nanoparticles, 2D material and conducting polymer modified electrode.
abstract: An essential biological sensor for acetylcholine (ACh) detection is constructed by immobilizing enzymes, acetylcholinesterase (AChE) and choline oxidase (ChO), on the surface of iron oxide nanoparticles (Fe2O3NPs), poly(3,4-ethylenedioxythiophene) (PEDOT)-reduced graphene oxide (rGO) nanocomposite modified fluorine doped tin oxide (FTO). The qualitative and quantitative measurements of nanocomposites properties were accomplished by scanning electron microscope (SEM), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). This prepared biological sensor delineated a wide linear range of 4.0nM to 800μM with a response time less than 4s and detection limit (based on S/N ratio) of 4.0nM. The sensor showed perfect sensitivity, excessive selectivity and stability for longer period of time during storage. Besides its very high-sensitivity, the biosensor has displayed a low detection limit which is reported for the first time in comparison to previously reported ACh sensors. By fabricating Fe2O3NPs/rGO/PEDOT modified FTO electrode for determining ACh level in serum samples, the applicability of biosensor has increased immensely as the detection of the level neurotransmitter is first priority for patients suffering from memory loss or Alzheimer's disease (AD).
authors.affiliation: Amity Institute of Nanotechnology, Amity University, Noida 201303, Uttar Pradesh, India.
authors.forename: Nidhi
authors.initials: N
authors.lastname: Chauhan
authors.affiliation: Disease Biology Laboratory, Regional Centre for Biotechnology, National Capital Region Biotech Science Cluster, Faridabad, Haryana, India.
authors.forename: Sheetal
authors.initials: S
authors.lastname: Chawla
authors.affiliation: Department of Biochemistry, M.D. University, Rohtak 124001, Haryana, India.
authors.forename: C S
authors.initials: CS
authors.lastname: Pundir
authors.affiliation: Amity Institute of Nanotechnology, Amity University, Noida 201303, Uttar Pradesh, India. Electronic address: utkarshmicromvp@gmail.com.
authors.forename: Utkarsh
authors.initials: U
authors.lastname: Jain
mesh_terms: D000109:Acetylcholine / Q000097:blood* / Q000378:metabolism; D000110:Acetylcholinesterase / Q000378:metabolism; D000421:Alcaligenes / Q000201:enzymology; D000429:Alcohol Oxidoreductases / Q000737:chemistry; D000544:Alzheimer Disease / Q000097:blood; D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry*; D004553:Electric Conductivity; D055664:Electrochemical Techniques / Q000379:methods; D004566:Electrodes; D004593:Electrophorus / Q000378:metabolism; D004800:Enzymes, Immobilized / Q000378:metabolism; D005290:Ferric Compounds / Q000737:chemistry; D029941:Fish Proteins / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D011108:Polymers / Q000737:chemistry*; D017971:Tin Compounds / Q000378:metabolism
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D004800:Enzymes, Immobilized; D005290:Ferric Compounds; D029941:Fish Proteins; D011108:Polymers; D017971:Tin Compounds; C121383:poly(3,4-ethylene dioxythiophene); C000499:ferric oxide; D006108:Graphite; D000429:Alcohol Oxidoreductases; C019888:choline oxidase; D000110:Acetylcholinesterase; C045358:stannic oxide; D000109:Acetylcholine
keywords: Acetylcholinesterase; Choline oxidase; Iron oxide nanoparticles, graphene oxide, poly(3,4-ethylenedioxythiophene)