title: The protective effect of melatonin on remote organ liver ischemia and reperfusion injury following aortic clamping.
abstract: BACKGROUND
  Severe local and systemic tissue injuries can occur after restoration of tissue oxygenation which is also known as reperfusion injury. Our objective was to investigate the possible protective effects of melatonin against IR damage in hepatic tissue following infrarenal aortic occlusion.
  
  
  METHODS
  A total of twenty-one male Wistar-albino rats separated into three groups as follows: Group I: Laparotomy and dissection of the infrarenal abdominal aorta (AA) were concurrently performed. Group II: About 1 ml of 0.9% saline was intraperitoenally administered 30 min before and after the occlusion operation. After laparotomy and dissection, infrarenal AA was clamped for 30 minutes and then was exposed to two hours of reperfusion. Group III: The melatonin was administered 30 min before clamping of the infrarenal AA then 30 min of ischemia and two hours of reperfusion was applied.
  
  
  RESULTS
  Serum aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase levels were remarkably higher in IR group, when compared with the sham group, and the laboratory tests returned to normal levels in IR+MEL group after treatment. Although serum IL-1β, IL-6, IL-18, TNF-α, and IFN- γ levels have decreased in treatment group following melatonin administration, this decrement was statistically significant for serum IL-18, TNF-α, and IFN- γ parameters compared with the IR group. Serum levels of TOC and OSI were decreased and tissue levels of TAC were increased by melatonin.
  
  
  CONCLUSIONS
  As a result of this study, it can be suggested that melatonin has antioxidant, anti-inflammatory and hepatoprotective effects in case of IR.
  
  
  BACKGROUND
  Aortic occlusion, Injury, Ischemia/Reperfusion, Liver, Melatonin.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Ozsoy
authors.forename: Yucel
authors.initials: Y
authors.lastname: Gonul
authors.forename: Ziya Taner
authors.initials: ZT
authors.lastname: Ozkececi
authors.forename: Ahmet
authors.initials: A
authors.lastname: Bali
authors.forename: Ruchan Bahadir
authors.initials: RB
authors.lastname: Celep
authors.forename: Ahmet
authors.initials: A
authors.lastname: Koçak
authors.forename: Fahri
authors.initials: F
authors.lastname: Adali
authors.forename: Murat
authors.initials: M
authors.lastname: Tosun
authors.forename: Sefa
authors.initials: S
authors.lastname: Celik
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D000975:Antioxidants / Q000627:therapeutic use*; D001012:Aorta, Abdominal / Q000601:surgery*; D001219:Aspartate Aminotransferases / Q000097:blood; D015415:Biomarkers; D003250:Constriction; D016207:Cytokines / Q000097:blood; D004353:Drug Evaluation, Preclinical; D007511:Ischemia / Q000150:complications* / Q000209:etiology; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008099:Liver / Q000098:blood supply*; D008550:Melatonin / Q000627:therapeutic use*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000209:etiology / Q000517:prevention & control*
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D015415:Biomarkers; D016207:Cytokines; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D008550:Melatonin