title: Silver_ nanoparticles inhibited erythrogenesis during zebrafish embryogenesis.
abstract: Silver_ nanoparticles (AgNPs), for their attractive antimicrobial properties, have become one of the most commercial nanomaterials used recently. AgNPs are reported to be toxic to blood cells of aquatic organisms and humans, however, few studies related to toxic effects of AgNPs in hematopoiesis using an in vivo model were available. Firstly, microarrays were applied to reveal transcriptional responses of zebrafish embryos to AgNPs at 24h post-fertilization (hpf)in this study, and hemoglobin genes were found to be down-regulated by AgNPs and to be enriched in the top 10 categories by Gene Ontology (GO) analysis. The reduced expressions of hemoglobin were further demonstrated by qRT-PCR detection, whole-mount in situ hybridization, and O-dianisidine staining at transcriptional and translational level. Next, the commitment of mesoderm, specification of hematopoietic progenitor cells and differentiation of erythroids were detected at different developmental stages in AgNPs-exposed embryos, and erythrogenesis were found to be inhibited by AgNPs in developmental-stage-specific and cell-specific manners. Finally, it was pointed out that AgNPs affected erythrogenesis mostly by their particles other than their releasing ions.
authors.affiliation: College of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, 430070, China. Electronic address: 1017015141@qq.com.
authors.forename: Bei
authors.initials: B
authors.lastname: Cui
authors.affiliation: College of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, 430070, China. Electronic address: renlong21@163.com.
authors.forename: Long
authors.initials: L
authors.lastname: Ren
authors.affiliation: College of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, 430070, China. Electronic address: 1559882908@qq.com.
authors.forename: Qin-Han
authors.initials: QH
authors.lastname: Xu
authors.affiliation: Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, Hainan University, HaiKou, 570228, China. Electronic address: lyyin@163.com.
authors.forename: Li-Yan
authors.initials: LY
authors.lastname: Yin
authors.affiliation: College of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, 430070, China. Electronic address: ameychow@yahoo.com.
authors.forename: Xin-Ying
authors.initials: XY
authors.lastname: Zhou
authors.affiliation: College of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, 430070, China; Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Hunan, Changde, 415000, China. Electronic address: ichliu@mail.hzau.edu.cn.
authors.forename: Jing-Xia
authors.initials: JX
authors.lastname: Liu
mesh_terms: D000818:Animals; D015536:Down-Regulation / Q000187:drug effects; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000502:physiology; D047108:Embryonic Development / Q000187:drug effects* / Q000502:physiology; D004920:Erythropoiesis / Q000187:drug effects* / Q000235:genetics; D005260:Female; D005819:Genetic Markers; D006454:Hemoglobins / Q000235:genetics; D008297:Male; D053768:Metal Nanoparticles / Q000633:toxicity*; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012834:Silver / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015027:Zebrafish
chemical_list: D005819:Genetic Markers; D006454:Hemoglobins; D014874:Water Pollutants, Chemical; D012834:Silver
keywords: AgNPs; Erythrogenesis; Hemoglobin; Microarray; Zebrafish