title: Mixed impact of Xpert(®) MTB/RIF on tuberculosis diagnosis in Cambodia.
abstract: METHODS
  National Tuberculosis (TB) Program sites in northwest Cambodia.
  
  
  OBJECTIVE
  To evaluate the impact of Xpert(®) MTB/RIF at point of care (POC) as compared to non-POC sites on the diagnostic evaluation of people living with the human immunodeficiency virus (PLHIV) with TB symptoms and patients with possible multidrug-resistant (MDR) TB.
  
  
  METHODS
  Observational cohort of patients undergoing routine diagnostic evaluation for TB following the rollout of Xpert.
  
  
  RESULTS
  Between October 2011 and June 2013, 431 of 822 (52%) PLHIV with TB symptoms and 240/493 (49%) patients with possible MDR-TB underwent Xpert. Xpert was more likely to be performed when available as POC. A smaller proportion of PLHIV at POC sites were diagnosed with TB than at non-POC sites; however, at POC sites, a higher proportion of those diagnosed with TB were bacteriologically positive. There was poor agreement between Xpert and other tests such as smear microscopy and culture. Overall, the evaluation of patients with possible MDR-TB increased following Xpert rollout, yet for patients confirmed as having drug resistance on drug susceptibility testing, only 46% had rifampin resistance that would be identified with Xpert.
  
  
  CONCLUSIONS
  Although utilization of Xpert was low, it may have contributed to an increase in evaluations for possible MDR-TB and a decline in empiric treatment for PLHIV when available as POC.
authors.affiliation: Division of Global HIV/TB, Centers for Disease Control and Prevention (CDC), Atlanta, Georgia, USA ; Division of Pulmonary and Critical Care Medicine, Emory University School of Medicine, Atlanta, Georgia, USA.
authors.forename: S C
authors.initials: SC
authors.lastname: Auld
authors.affiliation: Division of Global HIV/TB, Centers for Disease Control and Prevention (CDC), Atlanta, Georgia, USA.
authors.forename: B K
authors.initials: BK
authors.lastname: Moore
authors.affiliation: Department of Epidemiology, Biostatistics and Occupational Health, McGill University, Montreal, Quebec, Canada.
authors.forename: R P
authors.initials: RP
authors.lastname: Kyle
authors.affiliation: Division of Global HIV/TB, CDC, Phnom Penh, Cambodia.
authors.forename: B
authors.initials: B
authors.lastname: Eng
authors.affiliation: Division of Global HIV/TB, CDC, Phnom Penh, Cambodia.
authors.forename: K
authors.initials: K
authors.lastname: Nong
authors.affiliation: Division of Global HIV/TB, Centers for Disease Control and Prevention (CDC), Atlanta, Georgia, USA.
authors.forename: E S
authors.initials: ES
authors.lastname: Pevzner
authors.affiliation: National Center for Tuberculosis and Leprosy Control, Phnom Penh, Cambodia.
authors.forename: K K
authors.initials: KK
authors.lastname: Eam
authors.affiliation: National Center for Tuberculosis and Leprosy Control, Phnom Penh, Cambodia.
authors.forename: M T
authors.initials: MT
authors.lastname: Eang
authors.affiliation: Division of Global HIV/TB, CDC, Phnom Penh, Cambodia.
authors.forename: W P
authors.initials: WP
authors.lastname: Killam
keywords: HIV; MDR-TB; drug resistance; point-of-care testing