title: Variation in hemolytic activity of Brachyspira hyodysenteriae strains from pigs.
abstract: Brachyspira hyodysenteriae is the primary cause of swine dysentery, which is responsible for major economic losses to the pig industry worldwide. The hemolytic activity of 10 B. hyodysenteriae strains isolated from stools of pigs with mild to mucohemorrhagic diarrhea was compared and seven hemolysis associated genes were sequenced. Hemolysis induced by these strains varied from strong to near absent. One weakly hemolytic B. hyodysenteriae strain showed sequence changes in five hemolysis associated genes (tlyA, tlyB, hemolysin III, hemolysin activation protein and hemolysin III channel protein) resulting in amino acid substitutions. The occurrence of weakly hemolytic strains identifiable as B. hyodysenteriae should be taken into account in swine dysentery diagnostics. The presence of these strains may affect herd dysentery status, with great impact on a farms trading opportunities.
authors.affiliation: Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820, Merelbeke, Belgium.
authors.forename: Maxime
authors.initials: M
authors.lastname: Mahu
authors.affiliation: Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820, Merelbeke, Belgium.
authors.forename: Nele
authors.initials: N
authors.lastname: De Pauw
authors.affiliation: Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820, Merelbeke, Belgium.|Technology and Food Science Unit, Institute for Agricultural and Fisheries Research (ILVO), Brusselsesteenweg 370, B-9090, Melle, Belgium.
authors.forename: Lien
authors.initials: L
authors.lastname: Vande Maele
authors.affiliation: Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820, Merelbeke, Belgium.
authors.forename: Marc
authors.initials: M
authors.lastname: Verlinden
authors.affiliation: Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820, Merelbeke, Belgium.
authors.forename: Filip
authors.initials: F
authors.lastname: Boyen
authors.affiliation: Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820, Merelbeke, Belgium.
authors.forename: Richard
authors.initials: R
authors.lastname: Ducatelle
authors.affiliation: Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820, Merelbeke, Belgium.
authors.forename: Freddy
authors.initials: F
authors.lastname: Haesebrouck
authors.affiliation: Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820, Merelbeke, Belgium.
authors.forename: An
authors.initials: A
authors.lastname: Martel
authors.affiliation: Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820, Merelbeke, Belgium. frank.pasmans@ugent.be.
authors.forename: Frank
authors.initials: F
authors.lastname: Pasmans
mesh_terms: D019943:Amino Acid Substitution / Q000235:genetics; D000818:Animals; D017401:Brachyspira hyodysenteriae / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics / Q000502:physiology; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D006461:Hemolysis; D009097:Multienzyme Complexes / Q000235:genetics; D058885:Multilocus Sequence Typing / Q000662:veterinary; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D034421:Sus scrofa; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology* / Q000473:pathology
chemical_list: D009097:Multienzyme Complexes; D012336:RNA, Ribosomal, 16S; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases