title: Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts.
abstract: Human foreskin fibroblasts were used to generate the iPSC line iPSC-FH2.1 using the EF1a-hSTEMCCA-loxP vector expressing OCT4, SOX2, c-MYC and KLF4, in 5% O2 culture conditions. Stemness was confirmed, as was pluripotency both in vivo and in vitro, in normoxia and hypoxia. Human Embryonic Stem Cell (hESC) line WA-09 and reprogrammed fibroblast primary culture HFF-FM were used as controls.
authors.affiliation: LIAN-CONICET, FLENI, Ruta 9 Km 52.5, Belén de Escobar B1625XAF, Buenos Aires, Argentina; Laboratorio de Regulación de la Expresión Génica en Células Madre, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IQUIBICEN, CONICET-UBA, Intendente Güiraldes 2160, Ciudad Universitaria, Capital Federal, C1428EGA, Buenos Aires, Argentina.
authors.forename: María
authors.initials: M
authors.lastname: Questa
authors.affiliation: LIAN-CONICET, FLENI, Ruta 9 Km 52.5, Belén de Escobar B1625XAF, Buenos Aires, Argentina; Carrera de Investigador, CONICET, Argentina.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Romorini
authors.affiliation: LIAN-CONICET, FLENI, Ruta 9 Km 52.5, Belén de Escobar B1625XAF, Buenos Aires, Argentina.
authors.forename: Carolina
authors.initials: C
authors.lastname: Blüguermann
authors.affiliation: Laboratorio de Regulación de la Expresión Génica en Células Madre, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IQUIBICEN, CONICET-UBA, Intendente Güiraldes 2160, Ciudad Universitaria, Capital Federal, C1428EGA, Buenos Aires, Argentina.
authors.forename: Claudia María
authors.initials: CM
authors.lastname: Solari
authors.affiliation: LIAN-CONICET, FLENI, Ruta 9 Km 52.5, Belén de Escobar B1625XAF, Buenos Aires, Argentina.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Neiman
authors.affiliation: LIAN-CONICET, FLENI, Ruta 9 Km 52.5, Belén de Escobar B1625XAF, Buenos Aires, Argentina.
authors.forename: Carlos
authors.initials: C
authors.lastname: Luzzani
authors.affiliation: LIAN-CONICET, FLENI, Ruta 9 Km 52.5, Belén de Escobar B1625XAF, Buenos Aires, Argentina.
authors.forename: María Élida
authors.initials: MÉ
authors.lastname: Scassa
authors.affiliation: LIAN-CONICET, FLENI, Ruta 9 Km 52.5, Belén de Escobar B1625XAF, Buenos Aires, Argentina.
authors.forename: Gustavo Emilio
authors.initials: GE
authors.lastname: Sevlever
authors.affiliation: Laboratorio de Regulación de la Expresión Génica en Células Madre, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IQUIBICEN, CONICET-UBA, Intendente Güiraldes 2160, Ciudad Universitaria, Capital Federal, C1428EGA, Buenos Aires, Argentina; Carrera de Investigador, CONICET, Argentina; Departamento de Fisiología y Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires.
authors.forename: Alejandra Sonia
authors.initials: AS
authors.lastname: Guberman
authors.affiliation: LIAN-CONICET, FLENI, Ruta 9 Km 52.5, Belén de Escobar B1625XAF, Buenos Aires, Argentina; Carrera de Investigador, CONICET, Argentina.
authors.forename: Santiago Gabriel
authors.initials: SG
authors.lastname: Miriuka
mesh_terms: D002454:Cell Differentiation; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D055028:Comparative Genomic Hybridization; D019175:DNA Methylation; D005347:Fibroblasts / Q000166:cytology*; D052816:Foreskin / Q000166:cytology*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D059785:Karyotype; D000090062:Kruppel-Like Factor 4; D008297:Male; D008856:Microscopy, Fluorescence; D050814:Octamer Transcription Factor-3 / Q000235:genetics; D011401:Promoter Regions, Genetic; D060888:Real-Time Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D014157:Transcription Factors