title: [Relationship between Gene Polymorphism of HLA-A(*)/-B(*)-DRB1(*) and Aplastic Anemia in Chinese Han Population of Northwestern Plateau].
abstract: OBJECTIVE
  To investigate the correlation between the HLA genes and pathogenesis of aplastic anemia (AA), so as to find the susceptible AA genes.
  
  
  METHODS
  Polymerase chain reaction with specific sequence primers (PCR-SSP) method was used to detect the HLA typing of 50 AA patients and 183 normal healthy individuals as controls in Chinese Han population of northwestern plateau.
  
  
  RESULTS
  The frequency of HLA-A* 0201 (45.0%), B* 1501 (11.0%), B* 5501 (9.0%) and DRB1* 0901 (19.0%) gene frequences in AA patients were significantly higher than those in controls (Odds Ratio: OR=1.657, 2.138, 2.314 and 1.932, x2=4.882, 3.876, 3.863 and 4.473 (P<0.05). In contrast, A* 0301 gene frequency (4.0%) in AA was significantly lower than that in controls, OR=0.349, x2=4.154 (P<0.05). The male HLA-A* 0201 gene frequency was lower than that in female (38.2% vs 59.4%), and the difference was statistically significant (P<0.05). Concludsion: The HLA-A* 0201, B* 1501, B* 5501 and DRB1* 0901 genes may be considered as the risk markers while A* 0301 gene as a protective marker of AA, the HLA-A* 0201 also shows the sex differences.
authors.affiliation: Hematology Department and Institute of Hematology, Lanzhou General Hospital, Lanzhou Military Command, Lanzhou 730050, Gansu Province, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Yang
authors.affiliation: Animal Experiment Department, Lanzhou General Hospital, Lanzhou Military Command, Lanzhou 730050, Gansu Province, China.
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Guo
authors.affiliation: Department of Hematology, The Second Affiliated Hospital of Lanzhou University, Lanzhou 730050, Gansu Province, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Han
authors.affiliation: Hematology Department and Institute of Hematology, Lanzhou General Hospital, Lanzhou Military Command, Lanzhou 730050, Gansu Province, China.
authors.forename: Jian-Feng
authors.initials: JF
authors.lastname: Ou
authors.affiliation: Hematology Department and Institute of Hematology, Lanzhou General Hospital, Lanzhou Military Command, Lanzhou 730050, Gansu Province, China. E-mail: baihai98@hotmail.com.
authors.forename: Hai
authors.initials: H
authors.lastname: Bai
authors.affiliation: Hematology Department and Institute of Hematology, Lanzhou General Hospital, Lanzhou Military Command, Lanzhou 730050, Gansu Province, China.
authors.forename: Cun-Bang
authors.initials: CB
authors.lastname: Wang
authors.affiliation: Hematology Department and Institute of Hematology, Lanzhou General Hospital, Lanzhou Military Command, Lanzhou 730050, Gansu Province, China.
authors.forename: Yao-Zhu
authors.initials: YZ
authors.lastname: Pan
mesh_terms: D000483:Alleles; D000741:Anemia, Aplastic / Q000235:genetics*; D044466:Asian People / Q000235:genetics; D002681:China; D005260:Female; D005787:Gene Frequency; D059811:HLA-DRB1 Chains / Q000235:genetics*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
chemical_list: D059811:HLA-DRB1 Chains