title: Application of mouse model for effective evaluation of foot-and-mouth disease vaccine.
abstract: Efficacy evaluation of foot-and-mouth disease (FMD) vaccines has been conducted in target animals such as cows and pigs. In particular, handling FMD virus requires a high level of biosafety management and facilities to contain the virulent viruses. The lack of a laboratory animal model has resulted in inconvenience when it comes to using target animals for vaccine evaluation, bringing about increased cost, time and labor for the experiments. The FMD mouse model has been studied, but most FMD virus (FMDV) strains are not known to cause disease in adult mice. In the present study, we created a series of challenge viruses that are lethal to adult C57BL/6 mice. FMDV types O, A, and Asia1, which are related to frequent FMD outbreaks, were adapted for mice and the pathogenesis of each virus was evaluated in the mouse model. Challenge experiments after vaccination using in-house and commercial vaccines demonstrated vaccine-mediated protection in a dose-dependent manner. In conclusion, we propose that FMD vaccine evaluation should be carried out using mouse-adapted challenge viruses as a swift, effective efficacy test of experimental or commercial vaccines.
authors.affiliation: Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon City, Gyeongsangbuk-do 39660, Republic of Korea; College of Veterinary Medicine (BK21 Plus Program), Chungnam National University, 220 Gungdong, Daejon City 305-764, Republic of Korea.
authors.forename: Seo-Yong
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon City, Gyeongsangbuk-do 39660, Republic of Korea.
authors.forename: Mi-Kyeong
authors.initials: MK
authors.lastname: Ko
authors.affiliation: Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon City, Gyeongsangbuk-do 39660, Republic of Korea.
authors.forename: Kwang-Nyeong
authors.initials: KN
authors.lastname: Lee
authors.affiliation: Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon City, Gyeongsangbuk-do 39660, Republic of Korea.
authors.forename: Joo-Hyung
authors.initials: JH
authors.lastname: Choi
authors.affiliation: Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon City, Gyeongsangbuk-do 39660, Republic of Korea.
authors.forename: Su-Hwa
authors.initials: SH
authors.lastname: You
authors.affiliation: Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon City, Gyeongsangbuk-do 39660, Republic of Korea.
authors.forename: Hyun-Mi
authors.initials: HM
authors.lastname: Pyo
authors.affiliation: Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon City, Gyeongsangbuk-do 39660, Republic of Korea.
authors.forename: Myoung-Heon
authors.initials: MH
authors.lastname: Lee
authors.affiliation: Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon City, Gyeongsangbuk-do 39660, Republic of Korea.
authors.forename: Byounghan
authors.initials: B
authors.lastname: Kim
authors.affiliation: College of Veterinary Medicine (BK21 Plus Program), Chungnam National University, 220 Gungdong, Daejon City 305-764, Republic of Korea.
authors.forename: Jong-Soo
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon City, Gyeongsangbuk-do 39660, Republic of Korea. Electronic address: parkjhvet@korea.kr.
authors.forename: Jong-Hyeon
authors.initials: JH
authors.lastname: Park
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005536:Foot-and-Mouth Disease / Q000517:prevention & control*; D029544:Foot-and-Mouth Disease Virus / Q000145:classification*; D008810:Mice, Inbred C57BL; D065288:Serogroup; D015164:Vaccines, Inactivated / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D015164:Vaccines, Inactivated; D014765:Viral Vaccines
keywords: Challenge; Evaluation; Foot-and-mouth disease; Mouse FMD model; Mouse-adapted FMDV; Vaccine