title: E2-2, a novel immunohistochemical marker for both human and monkey plasmacytoid dendritic cells.
abstract: Plasmacytoid dendritic cells (pDCs) play important roles in initiating and regulating immune responses. pDC infiltration has been documented in multiple pathological lesions including infections, tumors, and autoimmune diseases, and the severity of pDC infiltration correlates with disease progression. However, a specific antibody for identifying pDCs by immunohistochemical staining on paraffin-embedded tissue sections is still lacking. Here, we developed a novel antibody targeted E2-2, a transcription factor preferentially expressed in pDCs. The antibody stains the nuclei of pDCs specifically in immunohistochemical analysis of various tissues from both human and rhesus monkey. This novel antibody will serve as a beneficial tool for pDC-related basic research and clinical investigation.
authors.affiliation: Key Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101 China.|University of Chinese Academy of Sciences, Beijing, 100080 China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Ma
authors.affiliation: Key Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101 China.|University of Chinese Academy of Sciences, Beijing, 100080 China.
authors.forename: Haisheng
authors.initials: H
authors.lastname: Yu
authors.affiliation: Key Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101 China.|University of Chinese Academy of Sciences, Beijing, 100080 China.
authors.forename: Xiangyun
authors.initials: X
authors.lastname: Yin
authors.affiliation: Key Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101 China.|University of Chinese Academy of Sciences, Beijing, 100080 China.
authors.forename: Menglan
authors.initials: M
authors.lastname: Cheng
authors.affiliation: Department of Cardiology, 306th Hospital of PLA, Beijing, 100101 China.
authors.forename: Quanxing
authors.initials: Q
authors.lastname: Shi
authors.affiliation: Department of Cardiology, 306th Hospital of PLA, Beijing, 100101 China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Yin
authors.affiliation: Key Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101 China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Nie
authors.affiliation: Department of Cardiology, 306th Hospital of PLA, Beijing, 100101 China.
authors.forename: Wang
authors.initials: W
authors.lastname: Shouli
authors.affiliation: Key Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101 China.
authors.forename: Liguo
authors.initials: L
authors.lastname: Zhang
keywords: E2-2; Immunohistochemistry; Monoclonal antibody; pDC