title: Regulation of the lysophosphatidylserine and sphingosine 1-phosphate levels in autologous whole blood by the pre-storage leukocyte reduction.
abstract: OBJECTIVE
  The effect of leukoreduction and storage periods on the accumulation of bioactive lysophospholipids and substances in human autologous blood (AB units) has not been fully investigated.
  
  
  METHODS
  The accumulation of bioactive lysophospholipids such as sphingosine 1-phosphate (S1P) and lysophosphatidylserine (LysoPS) in AB units during the storage was investigated. The time-dependent changes and the effect of the filtration in pre-storage leuckoreduction (LR) and unmodified samples derived from 46 AB units were analysed. Additionally, the changes of lysophospholipids and platelet releasate, namely β-thromboglobulin (β-TG), induced by exposure of whole blood (WB) or platelet-rich plasma (PRP) to the filter material were analysed.
  
  
  RESULTS
  LysoPS, but not S1P levels, time-dependently and significantly increased in both unmodified and LR samples. LysoPS significantly decreased in LR compared with unmodified samples, whereas S1P increased in LR compared with unmodified samples. In addition, exposure of WB and/or PRP to the filter material in vitro resulted in increased levels of S1P, LysoPS and β-TG.
  
  
  CONCLUSIONS
  LR effectively reduced the accumulation of LysoPS in AB units. On the other hand, it increased concentrations of S1P due to platelet activation by exposure to the filter material. These suggest that increases of S1P levels in LR and LysoPS in the unmodified samples were mainly caused by the leukocytes and/or platelets and that LR was effective in inhibiting the accumulation of LysoPS.
authors.affiliation: Department of Transfusion Medicine, The University of Tokyo Hospital, Tokyo, Japan. nagura-lab@h.u-tokyo.ac.jp.|Analytical Laboratory Chemistry, Graduate School of Health Care Sciences, Tokyo Medical and Dental University, Tokyo, Japan. nagura-lab@h.u-tokyo.ac.jp.
authors.forename: Y
authors.initials: Y
authors.lastname: Nagura
authors.affiliation: Department of Transfusion Medicine, The University of Tokyo Hospital, Tokyo, Japan.
authors.forename: N H
authors.initials: NH
authors.lastname: Tsuno
authors.affiliation: Graduate School of Pharmaceutical Sciences, Tohoku University, Miyagi, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kano
authors.affiliation: Graduate School of Pharmaceutical Sciences, Tohoku University, Miyagi, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Inoue
authors.affiliation: Graduate School of Pharmaceutical Sciences, Tohoku University, Miyagi, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Aoki
authors.affiliation: Laboratory Sciences, Department of Health Sciences, Saitama Prefectural University, Saitama, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Hirowatari
authors.affiliation: Department of Clinical Laboratory, The University of Tokyo Hospital, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kaneko
authors.affiliation: Department of Clinical Laboratory, The University of Tokyo Hospital, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kurano
authors.affiliation: Department of Transfusion Medicine, The University of Tokyo Hospital, Tokyo, Japan.|Laboratory Sciences, Department of Health Sciences, Saitama Prefectural University, Saitama, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Matsuhashi
authors.affiliation: Analytical Laboratory Chemistry, Graduate School of Health Care Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Ohkawa
authors.affiliation: Analytical Laboratory Chemistry, Graduate School of Health Care Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Tozuka
authors.affiliation: Department of Clinical Laboratory, The University of Tokyo Hospital, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Yatomi
authors.affiliation: Department of Transfusion Medicine, The University of Tokyo Hospital, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Okazaki
mesh_terms: D000328:Adult; D000368:Aged; D001793:Blood Preservation; D001804:Blood Transfusion, Autologous; D005260:Female; D006801:Humans; D047589:Leukocyte Reduction Procedures; D008246:Lysophospholipids / Q000097:blood*; D008297:Male; D008875:Middle Aged; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000097:blood
chemical_list: D008246:Lysophospholipids; C025059:lysophosphatidylserine; C060506:sphingosine 1-phosphate; D013110:Sphingosine
keywords: lysophosphatidylserine; pre-storage leukocyte reduction; sphingosine 1-phosphate