title: Purified rutin and rutin-rich asparagus attenuates disease severity and tissue damage following dextran sodium sulfate-induced colitis.
abstract: This study investigated the effects of cooked whole asparagus (ASP) versus its equivalent level of purified flavonoid glycoside, rutin (RUT), on dextran sodium sulfate (DSS)-induced colitis and subsequent colitis recovery in mice.
  
  
  
  C57BL/6 male mice were fed an AIN-93G basal diet (BD), or BD supplemented with 2% cooked ASP or 0.025% RUT for 2 wks prior to and during colitis induction with 2% DSS in water for 7 days, followed by 5 days colitis recovery. In colitic mice, both ASP and RUT upregulated mediators of improved barrier integrity and enhanced mucosal injury repair (e.g. Muc1, IL-22, Rho-A, Rac1, and Reg3γ), increased the proportion of mouse survival, and improved disease activity index. RUT had the greatest effect in attenuating DSS-induced colonic damage indicated by increased crypt and goblet cell restitution, reduced colonic myeloperoxidase, as well as attenuated DSS-induced microbial dysbiosis (reduced Enterobacteriaceae and Bacteroides, and increased unassigned Clostridales, Oscillospira, Lactobacillus, and Bifidobacterium).
  
  
  
  These findings demonstrate that dietary cooked ASP and its flavonoid glycoside, RUT, may be useful in attenuating colitis severity by modulating the colonic microenvironment resulting in reduced colonic inflammation, promotion of colonic mucosal injury repair, and attenuation of colitis-associated microbial dysbiosis.
authors.affiliation: Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph, ON, Canada.|Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, ON, Canada.
authors.forename: Krista A
authors.initials: KA
authors.lastname: Power
authors.affiliation: Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph, ON, Canada.|Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, ON, Canada.
authors.forename: Jenifer T
authors.initials: JT
authors.lastname: Lu
authors.affiliation: Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph, ON, Canada.|Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, ON, Canada.
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Monk
authors.affiliation: Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph, ON, Canada.
authors.forename: Dion
authors.initials: D
authors.lastname: Lepp
authors.affiliation: Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph, ON, Canada.
authors.forename: Wenqing
authors.initials: W
authors.lastname: Wu
authors.affiliation: Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph, ON, Canada.|Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, ON, Canada.
authors.forename: Claire
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph, ON, Canada.
authors.forename: Ronghua
authors.initials: R
authors.lastname: Liu
authors.affiliation: Guelph Food Research Centre, Agriculture and Agri-Food Canada, Guelph, ON, Canada.
authors.forename: Rong
authors.initials: R
authors.lastname: Tsao
authors.affiliation: Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, ON, Canada.
authors.forename: Lindsay E
authors.initials: LE
authors.lastname: Robinson
authors.affiliation: Department of Pathobiology, University of Guelph, Guelph, ON, Canada.
authors.forename: Geoffrey A
authors.initials: GA
authors.lastname: Wood
authors.affiliation: Department of Plant Agriculture, University of Guelph, Guelph, ON, Canada.
authors.forename: David J
authors.initials: DJ
authors.lastname: Wolyn
mesh_terms: D000818:Animals; D003092:Colitis / Q000139:chemically induced*; D003106:Colon / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D016264:Dextran Sulfate / Q000009:adverse effects*; D019587:Dietary Supplements; D004195:Disease Models, Animal; D007249:Inflammation; D007378:Interleukins / Q000378:metabolism; D007413:Intestinal Mucosa / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012431:Rutin / Q000032:analysis / Q000494:pharmacology*; D013431:Sulfates / Q000494:pharmacology*; D000097043:Interleukin-22
chemical_list: D016207:Cytokines; D016264:Dextran Sulfate; D007378:Interleukins; D012431:Rutin; D013431:Sulfates; D000097043:Interleukin-22; C012036:sodium sulfate
keywords: Asparagus; Colitis; Microbiota; Quercetin; Rutin