title: [Comparison of Biological Characteristics and Immunosuppressive Activity between Human Amniotic Mesenchymal Stem Cells and Human Bone Marrow Mesenchymal Stem Cells].
abstract: OBJECTIVE
  To compare the biological characteristics and immunosuppressive activity between human amniotic mesenchymal stem cells (hAMSC) and human bone marrow mesenchymal stem cells (hBMMSC).
  
  
  METHODS
  MSC from human amnion and bone marrow were isolated using enzymatic digestion and Ficoll-Hypaque density gradients, respectively. Their biological characteristics were compared by morphology, cell growth curves, cell cycle profile analysis, immunophenotype and immunofluorescence assay. Their immunosuppressive activities were studied on total activated T-cells with phytohemagglutinin (PHA-PBMSC). An in vitro co-culture was performed to compared the lymphocyte proliferation and the supernatant level of IFN-γ were measured by CCK-8 method and ELISA, respectively.
  
  
  RESULTS
  Both hAMSC and hBMMSC demonstrated fibroblast-like morphology. The hAMSC were able to be amplified for at least 15 passages, while the hBMMSC only for 6-7 passages. There was no significant difference in the proportion of G2/M phase cells of the 2 cells types (P>0.05). By FACS analysis for immunophenotype, both MSC were shown to be positive for CD105, CD90, CD73 and negative for CD34, CD45, CD11b, CD19, HLA-DR, but hAMSC were positive for Oct-3/4, which was in contrast to hBMMSC. Both of them expressed vimentin. Both the cells exhibited a inhibitory role on the lymphocyte proliferation induced by PHA in co-culture conditions, that was increased with the increase MSC proportion and both the suppressing effecs were enhanced. The supernatant IFN-γ levels of hAMSC co-cultured with lymphocyte at a ratio of 1:1 after 72 hours were measured by ELISA, and the level of IFN-γ was significantly lower than that in the same co-culture system of hBMMSC. In contrast to the IFN-γ in the PHA-stimulated group, the IFN-γ level in both co-culture groups was significantly lower.
  
  
  CONCLUSIONS
  MSC from amnion displayed a higher proliferative capacity and stem cell properties, compared with hBMMSC. Both MSC can inhibit lymphocyte proliferation and suppress IFN-γ secretion induced by PHA in vitro.
authors.affiliation: Department of Huiqiao, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China.|Co-first Authors: HONG Jai-Qiong and GAO Ya contributed equally to this work.
authors.forename: Jia-Qiong
authors.initials: JQ
authors.lastname: Hong
authors.affiliation: Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China.|Co-first Authors: HONG Jai-Qiong and GAO Ya contributed equally to this work.
authors.forename: Ya
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Huiqiao, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Song
authors.affiliation: Department of Huiqiao, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China.
authors.forename: Wei-Bin
authors.initials: WB
authors.lastname: Zhuo
authors.affiliation: Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China. E-mail: msunhaitao1988@126.com.
authors.forename: Hai-Tao
authors.initials: HT
authors.lastname: Sun
authors.affiliation: Department of Huiqiao, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China. E-mail: pbh66@126.com.
authors.forename: Bao-Hong
authors.initials: BH
authors.lastname: Ping
mesh_terms: D000650:Amnion / Q000166:cytology*; D001854:Bone Marrow Cells / Q000166:cytology; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D016130:Immunophenotyping; D007165:Immunosuppression Therapy; D008213:Lymphocyte Activation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D013601:T-Lymphocytes / Q000166:cytology