title: The LIKE SEX FOUR2 regulates root development by modulating reactive oxygen species homeostasis in Arabidopsis.
abstract: Maintaining reactive oxygen species (ROS) homeostasis plays a central role in plants, and is also critical for plant root development. Threshold levels of ROS act as signals for elongation and differentiation of root cells. The protein phosphatase LIKE SEX FOUR2 (LSF2) has been reported to regulate starch metabolism in Arabidopsis, but little is known about the mechanism how LSF2 affect ROS homeostasis. Here, we identified that LSF2 function as a component modulating ROS homeostasis in response to oxidative stress and, thus regulate root development. Compared with wild type Arabidopsis, lsf2-1 mutant exhibited reduced rates of superoxide generation and higher levels of hydrogen peroxide upon oxidative stress treatments. The activities of several antioxidant enzymes, including superoxide dismutase, catalase, and ascorbate peroxidase, were also affected in lsf2-1 mutant under these oxidative stress conditions. Consequently, lsf2-1 mutant exhibited the reduced root growth but less inhibition of root hair formation compared to wild type Arabidopsis plants. Importantly, protein phosphatase LSF2 interacted with mitogen-activated protein kinase 8 (MPK8), a known component of ROS homeostasis pathways in the cytoplasm. These findings indicated the novel function of LSF2 that controls ROS homeostasis to regulate root development.
authors.affiliation: NJU-NJFU Joint Institute for Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, China.
authors.forename: Pingzhi
authors.initials: P
authors.lastname: Zhao
authors.affiliation: NJU-NJFU Joint Institute for Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, China.
authors.forename: Lubomir N
authors.initials: LN
authors.lastname: Sokolov
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Ye
authors.affiliation: NJU-NJFU Joint Institute for Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, China.
authors.forename: Cheng-Yi
authors.initials: CY
authors.lastname: Tang
authors.affiliation: NJU-NJFU Joint Institute for Plant Molecular Biology, MOE Key Laboratory of Forest Genetics and Biotechnology, Nanjing Forestry University, Nanjing 210037, China.
authors.forename: Jisen
authors.initials: J
authors.lastname: Shi
authors.affiliation: NJU-NJFU Joint Institute for Plant Molecular Biology, MOE Key Laboratory of Forest Genetics and Biotechnology, Nanjing Forestry University, Nanjing 210037, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhen
authors.affiliation: NJU-NJFU Joint Institute for Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, China.
authors.forename: Wenzhi
authors.initials: W
authors.lastname: Lan
authors.affiliation: NJU-NJFU Joint Institute for Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Hong
authors.affiliation: NJU-NJFU Joint Institute for Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, China.
authors.forename: Jinliang
authors.initials: J
authors.lastname: Qi
authors.affiliation: NJU-NJFU Joint Institute for Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, China.
authors.forename: Gui-Hua
authors.initials: GH
authors.lastname: Lu
authors.affiliation: Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi 110021, India.
authors.forename: Girdhar K
authors.initials: GK
authors.lastname: Pandey
authors.affiliation: NJU-NJFU Joint Institute for Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, China.
authors.forename: Yong-Hua
authors.initials: YH
authors.lastname: Yang
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D054637:Dual-Specificity Phosphatases / Q000235:genetics / Q000378:metabolism*; D006706:Homeostasis / Q000502:physiology*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D009154:Mutation; D018384:Oxidative Stress / Q000502:physiology*; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development*
chemical_list: D029681:Arabidopsis Proteins; D006861:Hydrogen Peroxide; D054637:Dual-Specificity Phosphatases; C574400:LSF2 protein, Arabidopsis