title: Association of aberrant methylation at promoter regions of tumor suppressor genes with placental pathologies.
abstract: The resemblance between invasive behavior of cancer cells and placental trophoblasts and the role of aberrant epigenetic regulation in cancer development is well known.
  
  
  
  We analyzed the role of promoter region CpG-methylation and H3K9/27me3 of tumor suppressor genes in normal and pathological pregnancies and utilized their CpG-methylation data to search for fetal DNA epigenetic marker in maternal blood.
  
  
  
  CpG and H3K9/27-methylation associated decreased expression of RASSF1A and APC and increased expression of P16, RB1 and PRKCDBP was observed with advancing normal gestation. Gestational trophoblastic diseases and preeclampsia revealed gene-specific epigenetic deregulation of candidate tumor suppressor genes. Furthermore, APC and PRKCDBP showed the potential to act as fetal DNA epigenetic markers, similar to RASSF1A.
  
  
  
  Deregulation of methylation of tumor suppressor genes contributes to the development of preeclampsia and gestational trophoblastic diseases. APC and PRKCDBP may act as fetal DNA epigenetic markers for prenatal diagnosis.
authors.affiliation: Department of Biochemistry, Postgraduate Institute of Medical Education & Research, Chandigarh 160012, India.
authors.forename: Beenish
authors.initials: B
authors.lastname: Rahat
authors.affiliation: Department of Biochemistry, Postgraduate Institute of Medical Education & Research, Chandigarh 160012, India.
authors.forename: Shilpa
authors.initials: S
authors.lastname: Thakur
authors.affiliation: Cancer Pharmacology Division, CSIR-Indian Institute of Integrative Medicine, Jammu 180001, India.
authors.forename: Abid
authors.initials: A
authors.lastname: Hamid
authors.affiliation: Department of Obstetrics & Gynecology, Postgraduate Institute of Medical Education & Research, Chandigarh 160012, India.
authors.forename: Rashmi
authors.initials: R
authors.lastname: Bagga
authors.affiliation: Department of Biochemistry, Postgraduate Institute of Medical Education & Research, Chandigarh 160012, India.
authors.forename: Jyotdeep
authors.initials: J
authors.lastname: Kaur
mesh_terms: D025601:Adenomatous Polyposis Coli Protein / Q000235:genetics / Q000378:metabolism; D000328:Adult; D015415:Biomarkers / Q000097:blood; D002460:Cell Line; D018899:CpG Islands; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics / Q000378:metabolism; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D005260:Female; D016147:Genes, Tumor Suppressor; D031901:Gestational Trophoblastic Disease / Q000235:genetics*; D006657:Histones / Q000378:metabolism; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D010920:Placenta / Q000378:metabolism / Q000473:pathology; D011225:Pre-Eclampsia / Q000235:genetics*; D011247:Pregnancy; D011401:Promoter Regions, Genetic; D011499:Protein Processing, Post-Translational; D056286:Retinoblastoma Binding Proteins / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism; D055815:Young Adult
chemical_list: C555337:APC protein, human; D025601:Adenomatous Polyposis Coli Protein; D015415:Biomarkers; C505360:CAVIN3 protein, human; C000614131:CDKN2A protein, human; D019941:Cyclin-Dependent Kinase Inhibitor p16; D006657:Histones; D047908:Intracellular Signaling Peptides and Proteins; C411762:RASSF1 protein, human; C000606393:RB1 protein, human; D056286:Retinoblastoma Binding Proteins; D025521:Tumor Suppressor Proteins; D044767:Ubiquitin-Protein Ligases
keywords: APC; DNA methylation; GTDs; P16; PE; PRKCDBP; RASSF1A; RB1; histone modifications; placenta