title: Rapid Blue-Carba test: reduction in the detection time of carbapenemases performed from a 4-hour bacterial lawn.
abstract: The increase in carbapenem-resistant gram-negative bacteria is a matter of concern due to the limited therapeutic options available. In severe infections caused by these isolates, the rapid detection of the mechanisms of resistance is vital. We described a slightly modified version of the Blue-Carba test, rapid Blue-Carba test, which allows the detection of carbapenemases at 4 h of incubation from a haze of bacterial growth obtained from a positive blood culture. It was able to detect carbapenemase-producing isolates (Enterobacteriaceae, Pseudomonas aeruginosa and Acinetobacter baumannii) with a sensitivity and specificity of 98.1 and 100%, respectively. It is a rapid, easy-to-perform and an inexpensive technique that can be applied to routine laboratories, together with the simultaneous identification by mass spectrometry which would help to screen non-enzymatic carbapenem resistance; this method allows the detection of clinically relevant multidrug-resistant bacteria and the early implementation of accurate therapeutic interventions.
authors.affiliation: a Laboratorio de Bacteriología, Facultad de Farmacia y Bioquímica, Departamento de Bioquímica Clínica , Hospital de Clínicas José de San Martín, Universidad de Buenos Aires , Buenos Aires , Argentina.
authors.forename: Marcela
authors.initials: M
authors.lastname: Nastro
authors.affiliation: a Laboratorio de Bacteriología, Facultad de Farmacia y Bioquímica, Departamento de Bioquímica Clínica , Hospital de Clínicas José de San Martín, Universidad de Buenos Aires , Buenos Aires , Argentina.
authors.forename: Melisa
authors.initials: M
authors.lastname: Ayora
authors.affiliation: a Laboratorio de Bacteriología, Facultad de Farmacia y Bioquímica, Departamento de Bioquímica Clínica , Hospital de Clínicas José de San Martín, Universidad de Buenos Aires , Buenos Aires , Argentina.
authors.forename: Susana
authors.initials: S
authors.lastname: García
authors.affiliation: a Laboratorio de Bacteriología, Facultad de Farmacia y Bioquímica, Departamento de Bioquímica Clínica , Hospital de Clínicas José de San Martín, Universidad de Buenos Aires , Buenos Aires , Argentina.
authors.forename: Carlos
authors.initials: C
authors.lastname: Vay
authors.affiliation: a Laboratorio de Bacteriología, Facultad de Farmacia y Bioquímica, Departamento de Bioquímica Clínica , Hospital de Clínicas José de San Martín, Universidad de Buenos Aires , Buenos Aires , Argentina.
authors.forename: Ángela
authors.initials: Á
authors.lastname: Famiglietti
authors.affiliation: a Laboratorio de Bacteriología, Facultad de Farmacia y Bioquímica, Departamento de Bioquímica Clínica , Hospital de Clínicas José de San Martín, Universidad de Buenos Aires , Buenos Aires , Argentina.
authors.forename: Carlos Hernán
authors.initials: CH
authors.lastname: Rodriguez
mesh_terms: D000151:Acinetobacter Infections / Q000175:diagnosis / Q000188:drug therapy / Q000201:enzymology* / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use; D001118:Argentina; D016470:Bacteremia / Q000175:diagnosis / Q000188:drug therapy / Q000201:enzymology* / Q000382:microbiology; D001426:Bacterial Proteins / Q000097:blood* / Q000235:genetics; D015780:Carbapenems / Q000378:metabolism / Q000494:pharmacology / Q000627:therapeutic use; D024901:Drug Resistance, Multiple, Bacterial; D004755:Enterobacteriaceae / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000175:diagnosis / Q000188:drug therapy / Q000201:enzymology* / Q000382:microbiology; D006785:Hospitals, University; D006801:Humans; D008658:Inactivation, Metabolic; D058889:Molecular Typing; D011552:Pseudomonas Infections / Q000175:diagnosis / Q000188:drug therapy / Q000201:enzymology* / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D013997:Time Factors; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000097:blood* / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D015780:Carbapenems; D001618:beta-Lactamases; C063912:carbapenemase
keywords: Blood culture; Blue-Carba test; Carbapenemase; Detection time; Gram-negative bacteria; KPC