title: Spoligotyping of Mycobacterium tuberculosis Complex Isolates by Use of Ligation-Based Amplification and Melting Curve Analysis.
abstract: We report here a ligation-based spoligotyping that can identify unamplified spacers in membrane-based spoligotyping due to asymmetric insertion of IS6110 in the direct repeat locus. Our typing yielded 84.4% (411/487) concordance with traditional typing and 100% (487/487) accuracy when confirmed by DNA sequencing.
authors.affiliation: Engineering Research Centre of Molecular Diagnostics, Ministry of Education, State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian, China State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, Fujian, China Engineering Research Centre of Personalized Molecular Diagnostics of Xiamen, Fujian, China.
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Zeng
authors.affiliation: Tuberculosis Reference Laboratory, Henan Center for Disease Control and Prevention, Zhengzhou, Henan, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: Division of Tuberculosis Control and Prevention, Xiamen Center for Disease Control and Prevention, Xiamen, Fujian, China.
authors.forename: Rongrong
authors.initials: R
authors.lastname: Zheng
authors.affiliation: Public Health Research Institute, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA.
authors.forename: Natalia
authors.initials: N
authors.lastname: Kurepina
authors.affiliation: Public Health Research Institute, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA.
authors.forename: Barry N
authors.initials: BN
authors.lastname: Kreiswirth
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, Fujian, China Public Health Research Institute, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA.
authors.forename: Xilin
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Engineering Research Centre of Molecular Diagnostics, Ministry of Education, State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian, China State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, Fujian, China Engineering Research Centre of Personalized Molecular Diagnostics of Xiamen, Fujian, China xuye@xmu.edu.cn qgli@xmu.edu.cn.
authors.forename: Ye
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Engineering Research Centre of Molecular Diagnostics, Ministry of Education, State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian, China State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, Fujian, China Engineering Research Centre of Personalized Molecular Diagnostics of Xiamen, Fujian, China xuye@xmu.edu.cn qgli@xmu.edu.cn.
authors.forename: Qingge
authors.initials: Q
authors.lastname: Li
mesh_terms: D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D058889:Molecular Typing / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012091:Repetitive Sequences, Nucleic Acid; D044366:Transition Temperature
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial