title: A preliminary study of the oral microbiota in Chinese patients with Sjögren's syndrome.
abstract: OBJECTIVE
  To investigate the oral microbiota in Sjögren's syndrome (SS) as opposed to that of healthy subjects.
  
  
  METHODS
  Ten patients with primary SS, [6 patients daily taking stable dosage of hydroxychloroquine (HC) and 4 patients taking hydroxychloroquine combined with Prednisone acetas (HC+PA)], along with 10 age-matched healthy controls were examined in regard of number of teeth, stimulated/unstimulated saliva secretion rate. Microflora on bilateral buccal mucosa was analyzed by high throughput sequencing. Statistical analyses were performed using the chi-square test, t test and Mann-Whitney U test. The Venn diagrams and Redundancy Analysis (RDA) were also used to evaluate effects of the disease and treatment on the bacterial community composition.
  
  
  RESULTS
  The relative abundance of Proteobacteria in SS group was lower compared to controls (P=0.002). The total richness of genera for all groups was 339. The numbers of genera in SS group and in control group were 248 and 270, respectively. Some taxa with different prevalence and/or relative abundance were found between two groups.
  
  
  CONCLUSIONS
  SS affects the oral microbiota and SS patients carry a different and less diverse microorganism community compared with healthy subjects. Prednisone acetas is an influence on the oral microbiome. This study provides a basic data on the oral flora in SS patients.
authors.affiliation: Department of Stomatology, Zhongshan Hospital, Shanghai Medical School, Fudan University, 111 Yixueyuan Road, Shanghai 200032, China.
authors.forename: Miaoran
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Stomatology, Zhongshan Hospital, Shanghai Medical School, Fudan University, 111 Yixueyuan Road, Shanghai 200032, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zou
authors.affiliation: Department of Stomatology, Zhongshan Hospital, Shanghai Medical School, Fudan University, 111 Yixueyuan Road, Shanghai 200032, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Jiang
authors.affiliation: Department of Rheumatology, Zhongshan Hospital, Shanghai Medical School, Fudan University, 111 Yixueyuan Road, Shanghai 200032, China.
authors.forename: Lindi
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Department of Rheumatology, Zhongshan Hospital, Shanghai Medical School, Fudan University, 111 Yixueyuan Road, Shanghai 200032, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Yu
authors.affiliation: Department of Stomatology, Zhongshan Hospital, Shanghai Medical School, Fudan University, 111 Yixueyuan Road, Shanghai 200032, China. Electronic address: ding.xiaojun@zs-hospital.sh.cn.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Ding
authors.affiliation: Department of Stomatology, Zhongshan Hospital, Shanghai Medical School, Fudan University, 111 Yixueyuan Road, Shanghai 200032, China.
authors.forename: Youcheng
authors.initials: Y
authors.lastname: Yu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D004269:DNA, Bacterial / Q000302:isolation & purification; D005260:Female; D006801:Humans; D064307:Microbiota; D008875:Middle Aged; D009055:Mouth / Q000382:microbiology*; D009092:Mucous Membrane / Q000382:microbiology; D020560:Proteobacteria / Q000302:isolation & purification; D012472:Salivation; D017422:Sequence Analysis, DNA; D012859:Sjogren's Syndrome / Q000188:drug therapy / Q000382:microbiology*; D055815:Young Adult
chemical_list: D004269:DNA, Bacterial
keywords: Oral microbiome; Prednisone acetas; Sjögren’s syndrome