title: Basic fibroblast growth factor promotes melanocyte migration via activating PI3K/Akt-Rac1-FAK-JNK and ERK signaling pathways.
abstract: Vitiligo is a depigmentation disorder characterized by loss of functional melanocytes of the skin epidermis. The pathogenesis of vitiligo remains elusive. The purpose of this study is to investigate the effects of basic fibroblast growth factor (bFGF) on melanocyte migration, including its biochemical mechanism using transwell assay in vitro. We found that melanocyte treated with bFGF showed a significant increase in migration and cytoskeletal rearrangement. These changes were associated with increased activation of PI3K/Akt, Rac1, FAK, JNK, and ERK. Likewise, reduction of PI3K/Akt, Rac1, FAK, JNK, and ERK activity using selective inhibitors or siRNA was associated with impediment of bFGF-induced melanocyte migration. In addition, activity of Rac1, FAK, and JNK was reduced in cells in which PI3K/Akt was inhibited, activity of FAK and JNK was reduced in cells in which the Rac1 was inhibited, and activity of JNK was reduced in cells in which the FAK was inhibited. Collectively, these data demonstrate that bFGF facilitated melanocyte migration via PI3K/Akt-Rac1-FAK-JNK and ERK signaling pathways. © 2016 IUBMB Life, 68(9):735-747, 2016.
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, China.
authors.forename: Hongxue
authors.initials: H
authors.lastname: Shi
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, China.
authors.forename: Beibei
authors.initials: B
authors.lastname: Lin
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Wu
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Wound Healing and Cell Biology Laboratory, Institute of Basic Medical Science, Chinese PLA General Hospital, Beijing, China.
authors.forename: Cai
authors.initials: C
authors.lastname: Lin
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, China.
authors.forename: Zhouguang
authors.initials: Z
authors.lastname: Wang
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Zhu
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, China.
authors.forename: Yingzhen
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Wound Healing and Cell Biology Laboratory, Institute of Basic Medical Science, Chinese PLA General Hospital, Beijing, China.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Fu
authors.affiliation: Department of Otorhinolaryngology, The Affiliated YiWu Hospital, Wenzhou Medical University, Yiwu, China.
authors.forename: Zhencai
authors.initials: Z
authors.lastname: Lou
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, China.
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Xiao
mesh_terms: D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects* / Q000235:genetics; D004817:Epidermis / Q000187:drug effects / Q000473:pathology; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage / Q000235:genetics* / Q000378:metabolism; D051417:Focal Adhesion Kinase 1 / Q000235:genetics; D006801:Humans; D048670:MAP Kinase Kinase 4 / Q000235:genetics; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008544:Melanocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D014820:Vitiligo / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D020830:rac1 GTP-Binding Protein / Q000235:genetics
chemical_list: C107953:RAC1 protein, human; D034741:RNA, Small Interfering; D016222:Fibroblast Growth Factor 2; D019869:Phosphatidylinositol 3-Kinases; D051417:Focal Adhesion Kinase 1; C496050:PTK2 protein, human; D051057:Proto-Oncogene Proteins c-akt; D048670:MAP Kinase Kinase 4; D020830:rac1 GTP-Binding Protein
keywords: Rac-1; basic fibroblast growth factor; melanocytes; migration