title: The small 6C RNA of Corynebacterium glutamicum is involved in the SOS response.
abstract: The 6C RNA family is a class of small RNAs highly conserved in Actinobacteria, including the genera Mycobacterium, Streptomyces and Corynebacterium whose physiological function has not yet been elucidated. We found that strong transcription of the cgb_03605 gene, which encodes 6C RNA in C. glutamicum, was driven by the SigA- and SigB-dependent promoter Pcgb_03605. 6C RNA was detected at high level during exponential growth phase (180 to 240 molcules per cell) which even increased at the entry of the stationary phase. 6C RNA level did not decrease within 240 min after transcription had been stopped with rifampicin, which suggests high 6C RNA stability. The expression of cgb_03605 further increased approximately twofold in the presence of DNA-damaging mitomycin C (MMC) and nearly threefold in the absence of LexA. Deletion of the 6C RNA gene cgb_03605 resulted in a higher sensitivity of C. glutamicum toward MMC and UV radiation. These results indicate that 6C RNA is involved in the DNA damage response. Both 6C RNA level-dependent pausing of cell growth and branched cell morphology in response to MMC suggest that 6C RNA may also be involved in a control of cell division.
authors.affiliation: a Institute of Bio- and Geosciences IBG-1: Biotechnology, Forschungszentrum Jülich GmbH , Jülich , Germany.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Pahlke
authors.affiliation: b Institute of Microbiology of the CAS , v. v. i. Videnska, Czech Republic.
authors.forename: Hana
authors.initials: H
authors.lastname: Dostálová
authors.affiliation: b Institute of Microbiology of the CAS , v. v. i. Videnska, Czech Republic.
authors.forename: Jiří
authors.initials: J
authors.lastname: Holátko
authors.affiliation: a Institute of Bio- and Geosciences IBG-1: Biotechnology, Forschungszentrum Jülich GmbH , Jülich , Germany.
authors.forename: Ursula
authors.initials: U
authors.lastname: Degner
authors.affiliation: a Institute of Bio- and Geosciences IBG-1: Biotechnology, Forschungszentrum Jülich GmbH , Jülich , Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Bott
authors.affiliation: b Institute of Microbiology of the CAS , v. v. i. Videnska, Czech Republic.
authors.forename: Miroslav
authors.initials: M
authors.lastname: Pátek
authors.affiliation: a Institute of Bio- and Geosciences IBG-1: Biotechnology, Forschungszentrum Jülich GmbH , Jülich , Germany.
authors.forename: Tino
authors.initials: T
authors.lastname: Polen
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D048230:Corynebacterium glutamicum / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D020871:RNA Stability; D012329:RNA, Bacterial / Q000235:genetics*; D058727:RNA, Small Untranslated / Q000737:chemistry / Q000235:genetics*; D013014:SOS Response, Genetics / Q000235:genetics*; D012808:Sigma Factor / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D012329:RNA, Bacterial; D058727:RNA, Small Untranslated; D012808:Sigma Factor
keywords: 6C RNA; Actinobacteria; Corynebacterium glutamicum; RNA stability; SOS response; branched morphology; cell division; sigma factor