title: A bone morphogenetic protein ligand and receptors in mud crab: A potential role in the ovarian development.
abstract: In vertebrates, bone morphogenetic proteins (BMPs) play an important role in various biological processes. However, the function of BMPs in crustaceans is still unknown. In our study, a ligand (BMP7) and two receptors (Sp-BMPRIB and Sp-BMPRII) are cloned firstly in the mud crab, Scylla paramamosain. The qRT-PCR demonstrated that both ligand and receptors were expressed in various tissues, especially in ovary. The expression of BMPRs mRNA increased along the ovarian development, while BMP7 had an opposite tendency. In-situ hybridization revealed that Sp-BMPRIB and Sp-BMPRII were expressed in both oocytes and follicle cells, whereas Sp-BMP7 was exclusively localized in follicle cells. RNAi experiments showed that the expression levels of Smad1 and vitellogenin receptor declined rapidly after BMPRs were silenced. Based on these data, we hypothesized that in S. paramamosain, BMP7 and BMPRs had impact on the ovarian development, presumably via the autocrine/paracrine way.
authors.affiliation: College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Shu
authors.affiliation: College of the Environment & Ecology, Xiamen University, Xiamen 361102, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China.
authors.forename: Huiyang
authors.initials: H
authors.lastname: Huang
authors.affiliation: College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China; Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen 361102, China. Electronic address: haihuiye@xmu.edu.cn.
authors.forename: Haihui
authors.initials: H
authors.lastname: Ye
mesh_terms: D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics* / Q000378:metabolism; D052005:Bone Morphogenetic Protein Receptors, Type I / Q000235:genetics* / Q000378:metabolism; D052006:Bone Morphogenetic Protein Receptors, Type II / Q000235:genetics* / Q000378:metabolism; D019485:Bone Morphogenetic Proteins / Q000235:genetics* / Q000378:metabolism; D003386:Brachyura / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D005260:Female; D018507:Gene Expression Regulation, Developmental; D009865:Oocytes / Q000378:metabolism; D006080:Ovarian Follicle / Q000378:metabolism; D010053:Ovary / Q000254:growth & development / Q000378:metabolism; D017422:Sequence Analysis, DNA
chemical_list: D060829:Arthropod Proteins; D019485:Bone Morphogenetic Proteins; D052005:Bone Morphogenetic Protein Receptors, Type I; D052006:Bone Morphogenetic Protein Receptors, Type II
keywords: Bone morphogenetic protein; Crustacean; Ovarian development; Receptors