title: Simultaneous ammonia and nitrate removal in an airlift reactor using poly(butylene succinate) as carbon source and biofilm carrier.
abstract: In this study, an airlift inner-loop sequencing batch reactor using poly(butylene succinate) as the biofilm carrier and carbon source was operated under an alternant aerobic/anoxic strategy for nitrogen removal in recirculating aquaculture system. The average TAN and nitrate removal rates of 47.35±15.62gNH4-Nm(-3)d(-1) and 0.64±0.14kgNO3-Nm(-3)d(-1) were achieved with no obvious nitrite accumulation (0.70±0.76mg/L) and the dissolved organic carbon in effluents was maintained at 148.38±39.06mg/L. Besides, the activities of dissimilatory nitrate reduction to ammonium and sulfate reduction activities were successfully inhibited. The proteome KEGG analysis illustrated that ammonia might be removed through heterotrophic nitrification, while the activities of nitrate and nitrite reductases were enhanced through aeration treatment. The microbial community analysis revealed that denitrifiers of Azoarcus and Simplicispira occupied the dominate abundance which accounted for the high nitrate removal performance. Overall, this study broadened our understanding of simultaneous nitrification and denitrification using biodegradable material as biofilm carrier.
authors.affiliation: Institute of Agricultural Bio-Environmental Engineering, College of Bio-systems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China; Department of Biological and Environmental Engineering, Cornell University, Riley Robb Hall, Ithaca, NY 14853, USA.
authors.forename: Yun-Jie
authors.initials: YJ
authors.lastname: Ruan
authors.affiliation: Institute of Agricultural Bio-Environmental Engineering, College of Bio-systems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Ya-Le
authors.initials: YL
authors.lastname: Deng
authors.affiliation: Institute of Agricultural Bio-Environmental Engineering, College of Bio-systems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Xi-Shan
authors.initials: XS
authors.lastname: Guo
authors.affiliation: Department of Biological and Environmental Engineering, Cornell University, Riley Robb Hall, Ithaca, NY 14853, USA.
authors.forename: Michael B
authors.initials: MB
authors.lastname: Timmons
authors.affiliation: Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, China.
authors.forename: Hui-Feng
authors.initials: HF
authors.lastname: Lu
authors.affiliation: Institute of Agricultural Bio-Environmental Engineering, College of Bio-systems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Zhi-Ying
authors.initials: ZY
authors.lastname: Han
authors.affiliation: Institute of Agricultural Bio-Environmental Engineering, College of Bio-systems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Zhang-Ying
authors.initials: ZY
authors.lastname: Ye
authors.affiliation: Institute of Agricultural Bio-Environmental Engineering, College of Bio-systems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
authors.forename: Ming-Ming
authors.initials: MM
authors.lastname: Shi
authors.affiliation: Institute of Agricultural Bio-Environmental Engineering, College of Bio-systems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China. Electronic address: zhusm@zju.edu.cn.
authors.forename: Song-Ming
authors.initials: SM
authors.lastname: Zhu
mesh_terms: D000641:Ammonia / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D018441:Biofilms; D019149:Bioreactors; D002072:Butylene Glycols / Q000737:chemistry*; D058440:Denitrification; D009566:Nitrates / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D058465:Nitrification; D011108:Polymers / Q000737:chemistry*
chemical_list: D002072:Butylene Glycols; D009566:Nitrates; D011108:Polymers; C089797:bionole; D000641:Ammonia
keywords: Airlift reactor; Dissolved oxygen; Nitrogen removal; Recirculating aquaculture system; Salinity