title: Regulation of osteoclast differentiation by static magnetic fields.
abstract: Static magnetic field (SMF) modulates bone metabolism, but little research is concerned with the effects of SMF on osteoclast. Our previous studies show that osteogenic differentiation is strongly correlated with magnetic strength from hypo (500 nT), weak (geomagnetic field, GMF), moderate (0.2 T) to high (16 T) SMFs. We speculated that the intensity that had positive (16 T) or negative (500 nT and 0.2 T) effects on osteoblast differentiation would inversely influence osteoclast differentiation. To answer this question, we examined the profound effects of SMFs on osteoclast differentiation from pre-osteoclast Raw264.7 cells. Here, we demonstrated that 500 nT and 0.2 T SMFs promoted osteoclast differentiation, formation and resorption, while 16 T had an inhibitory effect. Almost all the osteoclastogenic genes were highly expressed under 500 nT and 0.2 T, including RANK, matrix metalloproteinase 9 (MMP9), V-ATPase, carbonic anhydrase II (Car2) and cathepsin K (CTSK), whereas they were decreased under 16 T. In addition, 16 T disrupted actin formation with remarkably decreased integrin β3 expression. Collectively, these results indicate that osteoclast differentiation could be regulated by altering the intensity of SMF, which is just contrary to that on osteoblast differentiation. Therefore, studies of SMF effects could reveal some parameters that could be used as a physical therapy for various bone disorders.
authors.affiliation: a School of Radiation Medicine and Protection , Medical College of Soochow University, Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions , Suzhou , P. R. China.|b Key Laboratory for Space Bioscience and Biotechnology , Institute of Special Environmental Biophysics, School of Life Sciences, Northwestern Polytechnical University , Xi'an , P. R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: b Key Laboratory for Space Bioscience and Biotechnology , Institute of Special Environmental Biophysics, School of Life Sciences, Northwestern Polytechnical University , Xi'an , P. R. China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Meng
authors.affiliation: b Key Laboratory for Space Bioscience and Biotechnology , Institute of Special Environmental Biophysics, School of Life Sciences, Northwestern Polytechnical University , Xi'an , P. R. China.
authors.forename: Chong
authors.initials: C
authors.lastname: Ding
authors.affiliation: b Key Laboratory for Space Bioscience and Biotechnology , Institute of Special Environmental Biophysics, School of Life Sciences, Northwestern Polytechnical University , Xi'an , P. R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Xie
authors.affiliation: b Key Laboratory for Space Bioscience and Biotechnology , Institute of Special Environmental Biophysics, School of Life Sciences, Northwestern Polytechnical University , Xi'an , P. R. China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Yang
authors.affiliation: b Key Laboratory for Space Bioscience and Biotechnology , Institute of Special Environmental Biophysics, School of Life Sciences, Northwestern Polytechnical University , Xi'an , P. R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: Shang
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D005786:Gene Expression Regulation; D060526:Magnetic Fields; D051379:Mice; D010010:Osteoclasts / Q000166:cytology* / Q000378:metabolism; D000067996:RAW 264.7 Cells
chemical_list: D000199:Actins
keywords: Bone resorption; cell growth; osteoclast formation, RAW264.7 cells; static magnetic fields