title: Neuroprotective effects of cold-inducible RNA-binding protein during mild hypothermia on traumatic brain injury.
abstract: Cold-inducible RNA-binding protein (CIRP), a key regulatory protein, could be facilitated by mild hypothermia in the brain, heart and liver. This study observed the effects of mild hypothermia at 31 ± 0.5°C on traumatic brain injury in rats. Results demonstrated that mild hypothermia suppressed apoptosis in the cortex, hippocampus and hypothalamus, facilitated CIRP mRNA and protein expression in these regions, especially in the hypothalamus. The anti-apoptotic effect of mild hypothermia disappeared after CIRP silencing. There was no correlation between mitogen-activated extracellular signal-regulated kinase activation and CIRP silencing. CIRP silencing inhibited extracellular signal-regulated kinase-1/2 activation. These indicate that CIRP inhibits apoptosis by affecting extracellular signal-regulated kinase-1/2 activation, and exerts a neuroprotective effect during mild hypothermia for traumatic brain injury.
authors.affiliation: Postgraduate Institution, Tianjin Medical University, Tianjin, China; Department of Neurosurgery, Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, China.
authors.forename: Guan
authors.identifier: 0000-0001-8310-7142
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, General Hospital of Tianjin Medical University, Tianjin, China.
authors.forename: Jian-Ning
authors.initials: JN
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, China.
authors.forename: Jia-Kui
authors.initials: JK
authors.lastname: Guo
authors.affiliation: Department of Neurosurgery, Tianjin Huanhu Hospital, Tianjin, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Department of Neurosurgery, Tianjin Huanhu Hospital, Tianjin, China.
authors.forename: Hong-Sheng
authors.initials: HS
authors.lastname: Sun
authors.affiliation: Department of Neurosurgery, Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Dong
authors.affiliation: Department of Neurosurgery, Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, China.
authors.forename: Cheng-Gang
authors.initials: CG
authors.lastname: Wu
keywords: anti-apoptosis; cold-inducible RNA-binding protein; mild hypothermia; mitogen-activated extracellular signal-regulated kinase; nerve regeneration; neural regeneration; traumatic brain injury