title: Differential responses of Trans-Resveratrol on proliferation of neural progenitor cells and aged rat hippocampal neurogenesis.
abstract: The plethora of literature has supported the potential benefits of Resveratrol (RV) as a life-extending as well as an anticancer compound. However, these two functional discrepancies resulted at different concentration ranges. Likewise, the role of Resveratrol on adult neurogenesis still remains controversial and less understood despite its well documented health benefits. To gather insight into the biological effects of RV on neurogenesis, we evaluated the possible effects of the compound on the proliferation and survival of neural progenitor cells (NPCs) in culture, and in the hippocampus of aged rats. Resveratrol exerted biphasic effects on NPCs; low concentrations (10 μM) stimulated cell proliferation mediated by increased phosphorylation of extracellular signal-regulated kinases (ERKs) and p38 kinases, whereas high concentrations (>20 μM) exhibited inhibitory effects. Administration of Resveratrol (20 mg/kg body weight) to adult rats significantly increased the number of newly generated cells in the hippocampus, with upregulation of p-CREB and SIRT1 proteins implicated in neuronal survival and lifespan extension respectively. We have successfully demonstrated that Resveratrol exhibits dose dependent discrepancies and at a lower concentration can have a positive impact on the proliferation, survival of NPCs and aged rat hippocampal neurogenesis implicating its potential as a candidate for restorative therapies against age related disorders.
authors.affiliation: System Toxicology and Health Risk Assessment Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), MG Marg, Lucknow, Uttar Pradesh-226001, India.
authors.forename: Vivek
authors.initials: V
authors.lastname: Kumar
authors.affiliation: System Toxicology and Health Risk Assessment Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), MG Marg, Lucknow, Uttar Pradesh-226001, India.
authors.forename: Ankita
authors.initials: A
authors.lastname: Pandey
authors.affiliation: System Toxicology and Health Risk Assessment Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), MG Marg, Lucknow, Uttar Pradesh-226001, India.
authors.forename: Sadaf
authors.initials: S
authors.lastname: Jahan
authors.affiliation: System Toxicology and Health Risk Assessment Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), MG Marg, Lucknow, Uttar Pradesh-226001, India.
authors.forename: Rajendra Kumar
authors.initials: RK
authors.lastname: Shukla
authors.affiliation: System Toxicology and Health Risk Assessment Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), MG Marg, Lucknow, Uttar Pradesh-226001, India.|Academy of Scientific &Innovative Research, CSIR-IITR Campus, Lucknow, India.
authors.forename: Dipak
authors.initials: D
authors.lastname: Kumar
authors.affiliation: System Toxicology and Health Risk Assessment Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), MG Marg, Lucknow, Uttar Pradesh-226001, India.
authors.forename: Akriti
authors.initials: A
authors.lastname: Srivastava
authors.affiliation: System Toxicology and Health Risk Assessment Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), MG Marg, Lucknow, Uttar Pradesh-226001, India.|Academy of Scientific &Innovative Research, CSIR-IITR Campus, Lucknow, India.
authors.forename: Shripriya
authors.initials: S
authors.lastname: Singh
authors.affiliation: System Toxicology and Health Risk Assessment Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), MG Marg, Lucknow, Uttar Pradesh-226001, India.|Academy of Scientific &Innovative Research, CSIR-IITR Campus, Lucknow, India.
authors.forename: Chetan Singh
authors.initials: CS
authors.lastname: Rajpurohit
authors.affiliation: System Toxicology and Health Risk Assessment Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), MG Marg, Lucknow, Uttar Pradesh-226001, India.|Academy of Scientific &Innovative Research, CSIR-IITR Campus, Lucknow, India.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Yadav
authors.affiliation: System Toxicology and Health Risk Assessment Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), MG Marg, Lucknow, Uttar Pradesh-226001, India.|Academy of Scientific &Innovative Research, CSIR-IITR Campus, Lucknow, India.
authors.forename: Vinay Kumar
authors.initials: VK
authors.lastname: Khanna
authors.affiliation: System Toxicology and Health Risk Assessment Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), MG Marg, Lucknow, Uttar Pradesh-226001, India.|Academy of Scientific &Innovative Research, CSIR-IITR Campus, Lucknow, India.
authors.forename: Aditya Bhushan
authors.initials: AB
authors.lastname: Pant
mesh_terms: D000072283:A549 Cells; D000375:Aging / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D058953:Neural Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D055495:Neurogenesis / Q000187:drug effects*; D010766:Phosphorylation / Q000187:drug effects; D051381:Rats; D000077185:Resveratrol; D056564:Sirtuin 1 / Q000378:metabolism; D013267:Stilbenes / Q000008:administration & dosage* / Q000494:pharmacology
chemical_list: C115163:CREB1 protein, human; D017362:Cyclic AMP Response Element-Binding Protein; D013267:Stilbenes; D048049:Extracellular Signal-Regulated MAP Kinases; C447939:SIRT1 protein, human; D056564:Sirtuin 1; D000077185:Resveratrol