title: Clinical Usefulness of Novel Immunochromatographic Detection Device for Porphyromonas gingivalis in Evaluating Effects of Scaling and Root Planing and Local Antimicrobial Therapy.
abstract: The authors have previously reported development of a novel immunochromatographic device (DK13-PG-001) for specific detection of Porphyromonas gingivalis (Pg). In this study, clinical usefulness of the detection device during periodontal therapy is presented.
  
  
  
  The multicenter study was conducted with 62 patients contributing 118 periodontitis sites with probing depth (PD) of 4 to 9 mm. Subgingival plaque samples were used for detection of Pg by DK13-PG-001 and the PCR-invader method at: 1) baseline (BL); 2) reevaluation (RE; after scaling and root planing); and 3) final evaluation (FE; after local drug delivery system). Periodontal examinations were performed concurrently with the test for Pg detection. Plasma immunoglobulin G (IgG) titers against Pg were also determined in patients using an enzyme-linked immunosorbent assay.
  
  
  
  DK13-PG-001 score and number of Pg by the PCR-invader method showed a strong correlation (r = 0.862) at three stages during periodontal therapy (n = 354). High sensitivity and specificity of DK13-PG-001, in comparison with the PCR-invader method, were shown. A significant correlation was found among device score, number of Pg by the PCR-invader method, and PD and clinical attachment level at BL and RE. Plasma IgG titers against Pg were significantly reduced at FE in comparison with BL. Weak but significant correlations between IgG titers and device scores were shown at BL and FE.
  
  
  
  Results suggest the DK13-PG-001 device is a useful tool for detection of Pg in dental offices and can aid clinical evaluation of the extent of periodontitis and therapeutic efficacy.
authors.affiliation: Department of Periodontology and Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Nakayama
authors.affiliation: Department of Periodontology and Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
authors.forename: Yorimasa
authors.initials: Y
authors.lastname: Ogata
authors.affiliation: Department of Periodontology and Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Hiromatsu
authors.affiliation: Department of Periodontology, Tokyo Dental College, Tokyo, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Imamura
authors.affiliation: Department of Periodontology, Tokyo Dental College, Tokyo, Japan.
authors.forename: Eiichi
authors.initials: E
authors.lastname: Suzuki
authors.affiliation: Department of Periodontology, Tokyo Dental College, Tokyo, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Saito
authors.affiliation: Department of Periodontology, Tsurumi University School of Dental Medicine, Yokohama, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Shirakawa
authors.affiliation: Department of Periodontology, Tsurumi University School of Dental Medicine, Yokohama, Japan.
authors.forename: Takatoshi
authors.initials: T
authors.lastname: Nagano
authors.affiliation: Department of Periodontology, Tsurumi University School of Dental Medicine, Yokohama, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Gomi
authors.affiliation: Division of Periodontology, Department of Oral Biological Science, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
authors.forename: Toshiya
authors.initials: T
authors.lastname: Morozumi
authors.affiliation: Research Institute, Showa Yakuhin Kako, Tokyo, Japan.
authors.forename: Kaori
authors.initials: K
authors.lastname: Watanabe
authors.affiliation: Reagent Research and Development Department, Denka Seiken, Gosen-shi, Niigata, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Akiishi
authors.affiliation: Division of Periodontology, Department of Oral Biological Science, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
authors.forename: Hiromasa
authors.initials: H
authors.lastname: Yoshie
mesh_terms: D016976:Aggregatibacter actinomycetemcomitans; D000890:Anti-Infective Agents; D001439:Bacteroides; D003773:Dental Plaque; D012534:Dental Scaling; D006801:Humans; D010514:Periodontal Pocket; D016966:Porphyromonas gingivalis; D016745:Root Planing
chemical_list: D000890:Anti-Infective Agents
keywords: Bacteria; Porphyromonas gingivalis; drug delivery systems; immunochromatography; periodontitis