title: Metabolic Labeling and Imaging of N-Linked Glycans in Arabidopsis Thaliana.
abstract: Molecular imaging of glycans has been actively pursued in animal systems for the past decades. However, visualization of plant glycans remains underdeveloped, despite that glycosylation is essential for the life cycle of plants. Metabolic glycan labeling in Arabidopsis thaliana by using N-azidoacetylglucosamine (GlcNAz) as the chemical reporter is reported. GlcNAz is metabolized through the salvage pathway of N-acetylglucosamine (GlcNAc) and incorporated into N-linked glycans, and possibly intracellular O-GlcNAc. Click-labeling with fluorescent probes enables visualization of newly synthesized N-linked glycans. N-glycosylation in the root tissue was discovered to possess distinct distribution patterns in different developmental zones, suggesting that N-glycosylation is regulated in a developmental stage-dependent manner. This work shows the utility of metabolic glycan labeling in elucidating the function of N-linked glycosylation in plants.
authors.affiliation: College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China.
authors.forename: Yuntao
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, 100871, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wu
authors.affiliation: College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China. xingchen@pku.edu.cn.|Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, 100871, China. xingchen@pku.edu.cn.|Synthetic and Functional Biomolecules Center, and Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University, Beijing, 100871, China. xingchen@pku.edu.cn.
authors.forename: Xing
authors.identifier: 0000-0002-3058-7370
authors.initials: X
authors.lastname: Chen
mesh_terms: D000117:Acetylglucosamine / Q000737:chemistry*; D017360:Arabidopsis / Q000737:chemistry* / Q000378:metabolism; D001386:Azides / Q000737:chemistry*; D006031:Glycosylation; D057054:Molecular Imaging; D011134:Polysaccharides / Q000032:analysis* / Q000378:metabolism*
chemical_list: D001386:Azides; D011134:Polysaccharides; D000117:Acetylglucosamine
keywords: Arabidopsis thaliana; N-linked glycans; metabolic labeling; plant glycobiology; salvage pathway