title: Seasonal and size-related variation of subcellular biomarkers in quagga mussels (Dreissena bugensis) inhabiting sites affected by moderate contamination with complex mixtures of pollutants.
abstract: The size-related differences in subcellular biomarker responses were assessed in Dreissena bugensis mussels inhabiting harbours moderately affected by pollution with complex mixtures of heavy metals and polycyclic aromatic hydrocarbons (PAHs). Adult D. bugensis samples were collected from three harbours of Lake Balaton (Hungary) characterized by moderate shipping activity, and as reference site, from a highly protected remote area of the lake. Biomarkers of exposure (metallothioneins (MTs), ethoxyresorufin-o-deethylase (EROD)), oxidative stress (lipid peroxidation (LPO), DNA strand breaks (DNAsb)) and possible endocrine disruption (vitellogenin-like proteins (VTG)) were analysed in whole-tissue homogenates of differently sized groups of mussels in relation to environmental parameters and priority pollutants (heavy metals and polycyclic aromatic hydrocarbons). Integrated biomarker response (IBR) indices were calculated for biomarker responses gained through in situ measurements to signalize critical sites and to better distinguish natural tendencies from biological effects of contaminants. Biomarker responses showed close positive correlation in case of MT, EROD, LPO, and DNAsb and negative correlation with VTG levels with mussel shell length in autumn, when higher levels of biomarkers appeared, possibly due to natural lifecycle changes of animals.
authors.affiliation: MTA ÖK Centre for Ecological Research, Balaton Limnological Institute, Klebelsberg K. u. 3, PO Box 35, Tihany, H-8237, Hungary.
authors.forename: A
authors.initials: A
authors.lastname: Ács
authors.affiliation: MTA ÖK Centre for Ecological Research, Balaton Limnological Institute, Klebelsberg K. u. 3, PO Box 35, Tihany, H-8237, Hungary.
authors.forename: Á
authors.initials: Á
authors.lastname: Vehovszky
authors.affiliation: MTA ÖK Centre for Ecological Research, Balaton Limnological Institute, Klebelsberg K. u. 3, PO Box 35, Tihany, H-8237, Hungary.
authors.forename: J
authors.initials: J
authors.lastname: Győri
authors.affiliation: MTA ÖK Centre for Ecological Research, Balaton Limnological Institute, Klebelsberg K. u. 3, PO Box 35, Tihany, H-8237, Hungary. farkas.anna@okologia.mta.hu.
authors.forename: A
authors.initials: A
authors.lastname: Farkas
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D045424:Complex Mixtures / Q000032:analysis* / Q000633:toxicity; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D004249:DNA Damage; D049875:Dreissena / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D004784:Environmental Monitoring / Q000379:methods*; D006814:Hungary; D060106:Lakes / Q000737:chemistry*; D015227:Lipid Peroxidation / Q000187:drug effects / Q000235:genetics; D008668:Metallothionein / Q000378:metabolism; D019216:Metals, Heavy / Q000032:analysis / Q000633:toxicity; D018384:Oxidative Stress / Q000187:drug effects / Q000235:genetics; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000633:toxicity; D012621:Seasons; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000633:toxicity
chemical_list: D015415:Biomarkers; D045424:Complex Mixtures; D019216:Metals, Heavy; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; D008668:Metallothionein; D019363:Cytochrome P-450 CYP1A1
keywords: Biochemical markers; DNA damage; Dreissena bugensis; Ethoxyresorufin-O-deethylase (EROD); Integrated biomarker response; Lipid peroxidation (LPO); Metallothionein-like proteins