title: Magicplex(TM) Sepsis Real-Time test to improve bloodstream infection diagnostics in children.
abstract: Sepsis is the major cause of morbidity and mortality in children, especially in immunocompromised patients, and a rapid identification of causative pathogen is strongly related with a better outcome. This prospective study analyzes the role of a multiplex real-time polymerase chain reaction in sepsis' etiological diagnosis. Magicplex(TM) Sepsis Real-Time tests were performed in tertiary Regina Margherita Children's Hospital (Turin, Italy), and the medical records of children who underwent a Magicplex test were prospectively evaluated. Results of the Magicplex test were compared with those of blood culture collected at a close time point. One hundred fifty Magicplex tests were collected from 89 patients (54 males and 35 females, age interquartile range: 2.6-12.1 years). Etiological definition was achieved in 60 bloodstream infection cases (40 %). In 32 episodes, Magicplex test alone gave a positive result, and blood culture alone permitted the etiological diagnosis in 5 septic episodes. Magicplex test allowed a 143 % increase in the diagnostic value of blood cultures.
  
  
  
  These results suggest that molecular biology can be useful for rapid pathogen's identification also in children.
  
  
  
  • Sepsis represents a major cause of morbidity and mortality in children. • Sepsis outcome is strongly related to rapid microbiological identification and prompt initiation of an appropriate chemotherapy. What is New: • This manuscript is the first that describes the use of Magicplex (TM) Sepsis Real-Time test in children. • The results suggest that molecular biology can be useful for rapid pathogen's identification also in children.
authors.affiliation: Department of Pediatrics, Infectious Diseases Unit, Regina Margherita Children' s Hospital, University of Turin, Piazza Polonia 94, Turin, 10126, Italy. marco.denina@gmail.com.
authors.forename: Marco
authors.initials: M
authors.lastname: Denina
authors.affiliation: Department of Pediatrics, Infectious Diseases Unit, Regina Margherita Children' s Hospital, University of Turin, Piazza Polonia 94, Turin, 10126, Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Scolfaro
authors.affiliation: Department of Pediatrics, Infectious Diseases Unit, Regina Margherita Children' s Hospital, University of Turin, Piazza Polonia 94, Turin, 10126, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Colombo
authors.affiliation: Department of Pediatrics, Infectious Diseases Unit, Regina Margherita Children' s Hospital, University of Turin, Piazza Polonia 94, Turin, 10126, Italy.
authors.forename: Carmelina
authors.initials: C
authors.lastname: Calitri
authors.affiliation: Department of Pediatrics, Infectious Diseases Unit, Regina Margherita Children' s Hospital, University of Turin, Piazza Polonia 94, Turin, 10126, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Garazzino
authors.affiliation: Department of Virology and Microbiology, A.O. Città della Salute e della Scienza (Molinette), University of Turin, Turin, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Barbui Anna
authors.affiliation: Department of Virology and Microbiology, A.O. Città della Salute e della Scienza (Molinette), University of Turin, Turin, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Brossa
authors.affiliation: Department of Pediatrics, Infectious Diseases Unit, Regina Margherita Children' s Hospital, University of Turin, Piazza Polonia 94, Turin, 10126, Italy.
authors.forename: Pier-Angelo
authors.initials: PA
authors.lastname: Tovo
mesh_terms: D016470:Bacteremia / Q000175:diagnosis*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D016867:Immunocompromised Host; D008297:Male; D011446:Prospective Studies; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors
keywords: Children; Diagnosis; Real-time polymerase chain reaction; Sepsis