title: The Escherichia coli P and Type 1 Pilus Assembly Chaperones PapD and FimC Are Monomeric in Solution.
abstract: The chaperone/usher pathway is used by Gram-negative bacteria to assemble adhesive surface structures known as pili or fimbriae. Uropathogenic strains of Escherichia coli use this pathway to assemble P and type 1 pili, which facilitate colonization of the kidney and bladder, respectively. Pilus assembly requires a periplasmic chaperone and outer membrane protein termed the usher. The chaperone allows folding of pilus subunits and escorts the subunits to the usher for polymerization into pili and secretion to the cell surface. Based on previous structures of mutant versions of the P pilus chaperone PapD, it was suggested that the chaperone dimerizes in the periplasm as a self-capping mechanism. Such dimerization is counterintuitive because the chaperone G1 strand, important for chaperone-subunit interaction, is buried at the dimer interface. Here, we show that the wild-type PapD chaperone also forms a dimer in the crystal lattice; however, the dimer interface is different from the previously solved structures. In contrast to the crystal structures, we found that both PapD and the type 1 pilus chaperone, FimC, are monomeric in solution. Our findings indicate that pilus chaperones do not sequester their G1 β-strand by forming a dimer. Instead, the chaperones may expose their G1 strand for facile interaction with pilus subunits. We also found that the type 1 pilus adhesin, FimH, is flexible in solution while in complex with its chaperone, whereas the P pilus adhesin, PapGII, is rigid. Our study clarifies a crucial step in pilus biogenesis and reveals pilus-specific differences that may relate to biological function.
  
  
  
  Pili are critical virulence factors for many bacterial pathogens. Uropathogenic E. coli relies on P and type 1 pili assembled by the chaperone/usher pathway to adhere to the urinary tract and establish infection. Studying pilus assembly is important for understanding mechanisms of protein secretion, as well as for identifying points for therapeutic intervention. Pilus biogenesis is a multistep process. This work investigates the oligomeric state of the pilus chaperone in the periplasm, which is important for understanding early assembly events. Our work unambiguously demonstrates that both PapD and FimC chaperones are monomeric in solution. We further demonstrate that the solution behavior of the FimH and PapGII adhesins differ, which may be related to functional differences between the two pilus systems.
authors.affiliation: Department of Biochemistry and Cell Biology, Stony Brook University, Stonybrook, New York, USA Biology Department, Brookhaven National Laboratory, Stonybrook, New York, USA.
authors.forename: Samema
authors.initials: S
authors.lastname: Sarowar
authors.affiliation: Department of Biochemistry and Cell Biology, Stony Brook University, Stonybrook, New York, USA Biology Department, Brookhaven National Laboratory, Stonybrook, New York, USA.
authors.forename: Olivia J
authors.initials: OJ
authors.lastname: Hu
authors.affiliation: Department of Molecular Genetics and Microbiology, Stony Brook University, Stonybrook, New York, USA Center for Infectious Diseases, Stony Brook University, Stonybrook, New York, USA.
authors.forename: Glenn T
authors.initials: GT
authors.lastname: Werneburg
authors.affiliation: Department of Molecular Genetics and Microbiology, Stony Brook University, Stonybrook, New York, USA Center for Infectious Diseases, Stony Brook University, Stonybrook, New York, USA.
authors.forename: David G
authors.initials: DG
authors.lastname: Thanassi
authors.affiliation: Department of Biochemistry and Cell Biology, Stony Brook University, Stonybrook, New York, USA Biology Department, Brookhaven National Laboratory, Stonybrook, New York, USA hli@bnl.gov.
authors.forename: Huilin
authors.initials: H
authors.lastname: Li
mesh_terms: D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D036781:Fimbriae Proteins / Q000235:genetics / Q000378:metabolism*; D010861:Fimbriae, Bacterial / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D008958:Models, Molecular; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism*; D033903:Periplasmic Proteins / Q000235:genetics / Q000378:metabolism*; D011487:Protein Conformation
chemical_list: D029968:Escherichia coli Proteins; D018832:Molecular Chaperones; C084330:PapD protein, E coli; D033903:Periplasmic Proteins; C489721:fimC protein, E coli; D036781:Fimbriae Proteins