title: The Project Of Another Low-Cost Metaphase Finder.
abstract: The most popular and 'gold standard' phenomenon in Biological dosimetry is the appearance of dicentric chromosomes in metaphase in white blood cells. The metaphase finder is a tool for biological dosimetry that finds metaphase cells on slide glasses. The author and a software company were using new special software that was faster than conventional systems. A Nikon Eclipse Ni-E microscope with motorised X-Y stage, 4× objective lens and 1920 × 1024 pixels colour camera for hardware were used. The software uses mathematical morphology filters. The new system was compact and low-priced. And the remarkable point is, this system can be applicable not only to human blood, but also to non-human samples. The speed was 208-236 s per 5 × 20 mm area, while capturing 378 images, which achieved the aim of the project. The false-positive ratio achieved below 5% in some slides.
authors.affiliation: National Institutes for Quantum and Radiological Science and Technology, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan furukawa.akira@qst.go.jp.
authors.forename: Akira
authors.initials: A
authors.lastname: Furukawa
mesh_terms: D002467:Cell Nucleus / Q000502:physiology / Q000648:ultrastructure*; D058948:Cell Tracking / Q000379:methods*; D002478:Cells, Cultured; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D000069550:Machine Learning; D008677:Metaphase / Q000502:physiology*; D008853:Microscopy / Q000379:methods*; D010363:Pattern Recognition, Automated / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014584:User-Computer Interface