title: [Optimization of Formulation of Panax notoginseng Saponins Transfersomes].
abstract: OBJECTIVE
  To optimize the formulation of Panax notoginseng saponins (PNS) transfersomes.
  
  
  METHODS
  PNS transfersomes were prepared by film hydration-dispersion process. Based on the entrapment efficiency (EE) of ginsenoside Rg1 and ginsenoside Rb1, the effects of formulated quantity of sodium deoxycholate and cholesterol, the relative ion strength and pH value of hydration liquid were investigated. The formulation of PNS transfersomes were optimized by single-factor experiment and uniform design experiment. The in vitro characteristics of the optimized transfersomes were evaluated.
  
  
  RESULTS
  The optimum formulation were as follows: egg phospholipid 0.45 g, cholesterol 0.05 g,vitamin E 0.01 g, sodium deoxycholate 0.119 g, PNS 0.1 g,10 mL of hydration liquid with pH at 4.75 (a mixture of 0.1 mol/L citric acid solution and 0.2 mol/L disodium orthophosphate solution, which corresponded to appropriate ion strength). The optimized PNS transfersomes had an average size of (121.8 ± 3.9) nm with a PDI of 0.136 ± 0.007 and a Zeta potential of (-8. 24 ± 0. 63) mV. The EE of ginsenoside Rg1 and ginsenoside Rb1 was 88.0% and 98.7% respectively.
  
  
  CONCLUSIONS
  The formulation of PNS transfersomes can be optimized by uniform design experiment combined with single-factor experiment.
authors.forename: Cui
authors.initials: C
authors.lastname: Chang
authors.forename: Si-si
authors.initials: SS
authors.lastname: Chen
authors.forename: Hong-tu
authors.initials: HT
authors.lastname: Yang
authors.forename: Hang-sheng
authors.initials: HS
authors.lastname: Zheng
mesh_terms: D002626:Chemistry, Pharmaceutical / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D036145:Ginsenosides / Q000737:chemistry; D053723:Panax notoginseng / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; D012503:Saponins; C442759:ginsenoside Rb1; C035054:ginsenoside Rg1