title: MicroRNA-214 exerts a Cardio-protective effect by inhibition of fibrosis.
abstract: The miRNAs play important roles in regulating myocardial fibrosis. The purpose of this study was to determine the potential roles of microRNA-214 (miR-214) in cardiac fibrosis in vitro and in vivo. In vitro experiment, Ang II-induced cardiac fibroblasts (CFBs) are transfected with pre-miR-214, anti-miR-214 and their oligo controls. Gene expression was checked by Quantitative realtime-PCR (qRT-PCR) and western blotting. In the present experiment, compared with controls, expressions of collagen type I (COL I), collagen type III (COL III), transforming growth factor (TGF)-β1, and tissue inhibitors of metalloproteinase (TIMP)-1 were all increased, but matrix metalloproteinase (MMP)-1 was reduced in CFB by Ang II treatment at both mRNA and protein levels, and these alterations were found reversed by miR-214 transfection. In vivo, an anterior transmural acute myocardial infarction (AMI) was created by occlusion of the left anterior descending coronary artery after Ad-pre-miR-214, Ad-anti-miR-214 or Ad-GFP was delivered separately. Four weeks after AMI, protein contents of COL I, COL III and TGF-β1 in tissue from border area were found increased after AMI, but impaired by overexpression of miR-214. While the expression of MMP-1 was increased by miR-214 stimulation but decreased by miR-214 inhibition. These results suggested that miR-214 exerts cardio-protective effects by inhibition of fibrosis and the inhibitory effect involves TGF-β1 suppression and MMP-1/TIMP-1 regulation. Anat Rec, 299:1348-1357, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Histology and Embryology, Hebei Medical University, Shijiazhuang, 050017, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Dong
authors.affiliation: Department of Emergency, the 3rd Hospital of Hebei Medical University, Shijiazhuang, 050051, China.
authors.forename: Shimin
authors.initials: S
authors.lastname: Dong
authors.affiliation: Department of Histology and Embryology, Hebei Medical University, Shijiazhuang, 050017, China.
authors.forename: Lisi
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Emergency, the 3rd Hospital of Hebei Medical University, Shijiazhuang, 050051, China.
authors.forename: Xueping
authors.initials: X
authors.lastname: Gao
authors.affiliation: Department of Emergency, the 3rd Hospital of Hebei Medical University, Shijiazhuang, 050051, China.
authors.forename: Guangwei
authors.initials: G
authors.lastname: Lv
authors.affiliation: Department of Histology and Embryology, Hebei Medical University, Shijiazhuang, 050017, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Histology and Embryology, Hebei Medical University, Shijiazhuang, 050017, China. shaosuxia1202@126.com.
authors.forename: Suxia
authors.initials: S
authors.lastname: Shao
mesh_terms: D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D024042:Collagen Type I / Q000378:metabolism; D024061:Collagen Type III / Q000378:metabolism; D004195:Disease Models, Animal; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D005355:Fibrosis / Q000378:metabolism* / Q000473:pathology; D006321:Heart / Q000187:drug effects*; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009203:Myocardial Infarction / Q000378:metabolism* / Q000473:pathology; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism; D014162:Transfection; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III; D035683:MicroRNAs; C559947:Mirn214 microRNA, rat; D019715:Tissue Inhibitor of Metalloproteinase-1; D053773:Transforming Growth Factor beta1; D000804:Angiotensin II
keywords: MMP-1/TIMP-1; TGF-β1; collagen; miR-214; myocardial fibrosis