title: Evaluation of serological response to foot-and-mouth disease vaccination in BLV infected cows.
abstract: BACKGROUND
  Bovine Leukemia Virus (BLV) produces disorders on the immune system in naturally infected animals, which may counteract the development of immunity after vaccination. The aim of this study was to investigate whether healthy and BLV infected cattle elicited similar humoral responses after foot and mouth disease (FMD) immunization. In a field study, 35 Holstein heifers were selected based on their BLV serological status and immunized with a single dose of a commercial bivalent oil-based FMD vaccine. Serum samples were collected at 0, 15, 60, 165 and 300 days post vaccination (dpv).
  
  
  RESULTS
  Total anti-A24/Cruzeiro antibodies, IgM, IgG1, IgG2 titers and avidity index of specific antibodies were determined by ELISA. Although only marginally significant differences were found between groups in terms of total antibodies, anti-FMD IgM and IgG1 titers were significantly lower in heifers infected with BLV at the 15 dpv (p < 0.01). Animals that became infected during the study did not show differences to the BLV negative group.
  
  
  CONCLUSIONS
  Cattle infected with BLV at the time of immunization may elicit a low-magnitude serological response to a commercial Foot-and-mouth disease vaccine.
authors.affiliation: Immunology Area - Department of Microbiological Sciences, Faculty of Veterinary, University of the Republic (UdelaR), Montevideo, Uruguay. rpuentes@adinet.com.uy.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Puentes
authors.affiliation: Immunology Area - Department of Microbiological Sciences, Faculty of Veterinary, University of the Republic (UdelaR), Montevideo, Uruguay.
authors.forename: Laureana
authors.initials: L
authors.lastname: De Brun
authors.affiliation: Immunology Area - Department of Microbiological Sciences, Faculty of Veterinary, University of the Republic (UdelaR), Montevideo, Uruguay.
authors.forename: Agustina
authors.initials: A
authors.lastname: Algorta
authors.affiliation: Immunology Area - Department of Microbiological Sciences, Faculty of Veterinary, University of the Republic (UdelaR), Montevideo, Uruguay.
authors.forename: Valeria
authors.initials: V
authors.lastname: Da Silva
authors.affiliation: Centre of Veterinary Sciences and Agronomic Investigations, INTA, Virology Institute, Buenos Aires, Argentina.
authors.forename: Florencia
authors.initials: F
authors.lastname: Mansilla
authors.affiliation: Independent Veterinary Practice, Florida, Uruguay.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Sacco
authors.affiliation: Genetics Area, Faculty of Veterinary, University of the Republic (UdelaR), Montevideo, Uruguay.
authors.forename: Silvia
authors.initials: S
authors.lastname: Llambí
authors.affiliation: Centre of Veterinary Sciences and Agronomic Investigations, INTA, Virology Institute, Buenos Aires, Argentina.|CONICET - National Council of Scientific and Technological Research, Buenos Aires, Argentina.
authors.forename: Alejandra V
authors.initials: AV
authors.lastname: Capozzo
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D016583:Enzootic Bovine Leukosis / Q000276:immunology* / Q000821:virology; D005260:Female; D029544:Foot-and-Mouth Disease Virus / Q000276:immunology*; D001909:Leukemia Virus, Bovine; D015164:Vaccines, Inactivated / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D015164:Vaccines, Inactivated; D014765:Viral Vaccines
keywords: BLV; Dairy cattle; FMD; Immune response; Immunization; Serology