title: Dopamine D3 receptors modulate the rate of neuronal recovery, cell recruitment in Area X, and song tempo after neurotoxic damage in songbirds.
abstract: Songbirds, like humans, learn vocalizations and their striatum recruits new neurons in adulthood. Injury in striatal vocal nucleus Area X, involved in song learning and production in songbirds, is followed by massive regeneration. The newborn neurons arise from the subventricular zone (SVZ) rich in dopamine D3 receptors (D3Rs). The aim of this study was to investigate whether the D3Rs affect the rate of neuronal recovery in Area X. Male zebra finches (Taeniopygia guttata) received bilateral neurotoxic lesion of Area X and were implanted with osmotic minipumps containing D3R agonist 7-OH-DPAT, antagonist U99194, or saline. Treatment with 7-OH-DPAT but not U99194 led to significant reduction of lesion size and increased numbers of migrating neuroblasts and newborn cells in the Area X. These cells were detected in the lesion border as well as the lesion center. Lesion also led to increased mRNA expression of the D3Rs in the neurogenic SVZ and in the nucleus robustus arcopallialis (RA) involved in song production. Moreover, lesion alone prolonged the song duration and this may be facilitated by D3Rs in RA. Parallel lesion and stimulation of D3Rs prolonged it even more, while blocking of D3Rs abolished the lesion-induced effect. These data suggest that D3R stimulation after striatal injury accelerates the striatal recovery and can cause behavioral alterations.
authors.affiliation: Department of Physiology and Ethology, Institute of Animal Biochemistry and Genetics, Slovakia.
authors.forename: Kristina
authors.initials: K
authors.lastname: Lukacova
authors.affiliation: Department of Physiology and Ethology, Institute of Animal Biochemistry and Genetics, Slovakia.
authors.forename: Eva
authors.initials: E
authors.lastname: Pavukova
authors.affiliation: Department of Physiology and Ethology, Institute of Animal Biochemistry and Genetics, Slovakia.
authors.forename: Lubor
authors.initials: L
authors.lastname: Kostal
authors.affiliation: Department of Physiology and Ethology, Institute of Animal Biochemistry and Genetics, Slovakia.
authors.forename: Boris
authors.initials: B
authors.lastname: Bilcik
authors.affiliation: Department of Physiology and Ethology, Institute of Animal Biochemistry and Genetics, Slovakia. Electronic address: Lubica.Kubikova@savba.sk.
authors.forename: Lubica
authors.initials: L
authors.lastname: Kubikova
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D003342:Corpus Striatum / Q000187:drug effects / Q000293:injuries* / Q000378:metabolism* / Q000473:pathology; D004195:Disease Models, Animal; D015259:Dopamine Agents / Q000494:pharmacology; D046369:Finches / Q000502:physiology*; D007051:Ibotenic Acid; D007189:Indans / Q000494:pharmacology; D008297:Male; D058953:Neural Stem Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D055495:Neurogenesis / Q000187:drug effects / Q000502:physiology; D009473:Neuronal Plasticity / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D050637:Receptors, Dopamine D3 / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism*; D020127:Recovery of Function / Q000187:drug effects / Q000502:physiology; D055153:Stem Cell Niche / Q000187:drug effects / Q000502:physiology; D013764:Tetrahydronaphthalenes / Q000494:pharmacology; D014828:Vocalization, Animal / Q000187:drug effects / Q000502:physiology*
chemical_list: D030161:Avian Proteins; D015259:Dopamine Agents; D007189:Indans; D012333:RNA, Messenger; D050637:Receptors, Dopamine D3; D013764:Tetrahydronaphthalenes; C086335:(5,6-dimethoxyindan-2-yl)dipropylamine; D007051:Ibotenic Acid; C031349:7-hydroxy-2-N,N-dipropylaminotetralin
keywords: brain injury; dopamine D3 receptors; song tempo; striatum; zebra finch