title: Selection and characterization of Beauveria spp. isolates to control the broad mite Polyphagotarsonemus latus (Banks, 1904) (Acari: Tarsonemidae).
abstract: This study was performed under laboratory conditions to identify isolates of the fungus Beauveria spp. that can control Polyphagotarsonemus latus in the greenhouse and field. Thirty Beauveria spp. isolates were tested by spraying 1 mL conidia (1 × 108 conidia/mL) on pepper leaf discs containing 15 mites. Evaluations were performed on the 3rd and 6th day post application by counting the number of dead mites. Vegetative growth and conidial production were measured from the selected isolates, and bioassays were conducted in the greenhouse on bean seedlings in plastic pots. The isolate Unioeste 53 was selected, and a conidial suspension (1 × 108 conidia/mL) was applied with a backpack sprayer. The evaluation consisted of pre- and post-treatment counts of the number of live mites on ten leaflets in both the plots treated with the fungus and control plots, and the same procedure was followed for the field experiment. In the laboratory, the Unioeste 53 isolate resulted in total and confirmed mortality rates of 70% and 57.7%, respectively. In the greenhouse, the population decreased by 76.71% by the 16th day after application. In the field, the population decreased by 66% by the 12th day after application, demonstrating the potential of this fungus for mite management.
authors.affiliation: Laboratório de Biotecnologia Agrícola, Universidade Estadual do Oeste do Paraná, Universidade Estadual do Oeste do Paraná, Cascavel PR , Brazil, Programa de Pós-graduação em Conservação e Manejo de Recursos Naturais, Laboratório de Biotecnologia Agrícola, Universidade Estadual do Oeste do Paraná - Unioeste, Rua Universitária, 2069, Jardim Universitário, CEP 85819-110, Cascavel, PR, Brazil.
authors.forename: C C
authors.initials: CC
authors.lastname: Martins
authors.affiliation: Laboratório de Biotecnologia Agrícola, Universidade Estadual do Oeste do Paraná, Universidade Estadual do Oeste do Paraná, Cascavel PR , Brazil, Programa de Pós-graduação em Conservação e Manejo de Recursos Naturais, Laboratório de Biotecnologia Agrícola, Universidade Estadual do Oeste do Paraná - Unioeste, Rua Universitária, 2069, Jardim Universitário, CEP 85819-110, Cascavel, PR, Brazil.
authors.forename: L F A
authors.initials: LF
authors.lastname: Alves
authors.affiliation: Laboratório de Biotecnologia Agrícola, Universidade Estadual do Oeste do Paraná, Universidade Estadual do Oeste do Paraná, Cascavel PR , Brazil, Programa de Pós-graduação em Conservação e Manejo de Recursos Naturais, Laboratório de Biotecnologia Agrícola, Universidade Estadual do Oeste do Paraná - Unioeste, Rua Universitária, 2069, Jardim Universitário, CEP 85819-110, Cascavel, PR, Brazil.
authors.forename: A P
authors.initials: AP
authors.lastname: Mamprim
authors.affiliation: Laboratório de Biotecnologia Agrícola, Universidade Estadual do Oeste do Paraná, Universidade Estadual do Oeste do Paraná, Cascavel PR , Brazil, Programa de Pós-graduação em Conservação e Manejo de Recursos Naturais, Laboratório de Biotecnologia Agrícola, Universidade Estadual do Oeste do Paraná - Unioeste, Rua Universitária, 2069, Jardim Universitário, CEP 85819-110, Cascavel, PR, Brazil.
authors.forename: L P A
authors.initials: LP
authors.lastname: Souza
mesh_terms: D000818:Animals; D052982:Beauveria / Q000302:isolation & purification*; D008925:Mites / Q000187:drug effects*; D010936:Plant Extracts / Q000627:therapeutic use*; D013172:Spores, Fungal / Q000302:isolation & purification
chemical_list: D010936:Plant Extracts