title: BC1 RNA motifs required for dendritic transport in vivo.
abstract: BC1 RNA is a small brain specific non-protein coding RNA. It is transported from the cell body into dendrites where it is involved in the fine-tuning translational control. Due to its compactness and established secondary structure, BC1 RNA is an ideal model for investigating the motifs necessary for dendritic localization. Previously, microinjection of in vitro transcribed BC1 RNA mutants into the soma of cultured primary neurons suggested the importance of RNA motifs for dendritic targeting. These ex vivo experiments identified a single bulged nucleotide (U22) and a putative K-turn (GA motif) structure required for dendritic localization or distal transport, respectively. We generated six transgenic mouse lines (three founders each) containing neuronally expressing BC1 RNA variants on a BC1 RNA knockout mouse background. In contrast to ex vivo data, we did not find indications of reduction or abolition of dendritic BC1 RNA localization in the mutants devoid of the GA motif or the bulged nucleotide. We confirmed the ex vivo data, which showed that the triloop terminal sequence had no consequence on dendritic transport. Interestingly, changing the triloop supporting structure completely abolished dendritic localization of BC1 RNA. We propose a novel RNA motif important for dendritic transport in vivo.
authors.affiliation: Institute of Experimental Pathology, Center for Molecular Biology of Inflammation (ZMBE), University of Münster, Von-Esmarch-Str. 56, D-48149, Münster, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Robeck
authors.affiliation: Institute of Experimental Pathology, Center for Molecular Biology of Inflammation (ZMBE), University of Münster, Von-Esmarch-Str. 56, D-48149, Münster, Germany.|Department of Medicine (TRAM), University of Münster, Von-Esmarch-Str. 56, D-48149 Münster, Germany.
authors.forename: Boris V
authors.initials: BV
authors.lastname: Skryabin
authors.affiliation: Institute of Experimental Pathology, Center for Molecular Biology of Inflammation (ZMBE), University of Münster, Von-Esmarch-Str. 56, D-48149, Münster, Germany.|Department of Medicine (TRAM), University of Münster, Von-Esmarch-Str. 56, D-48149 Münster, Germany.
authors.forename: Timofey S
authors.initials: TS
authors.lastname: Rozhdestvensky
authors.affiliation: Institute of Experimental Pathology, Center for Molecular Biology of Inflammation (ZMBE), University of Münster, Von-Esmarch-Str. 56, D-48149, Münster, Germany.
authors.forename: Anastasiya B
authors.initials: AB
authors.lastname: Skryabin
authors.affiliation: Institute of Experimental Pathology, Center for Molecular Biology of Inflammation (ZMBE), University of Münster, Von-Esmarch-Str. 56, D-48149, Münster, Germany.|Institute of Evolutionary and Medical Genomics, Brandenburg Medical School (MHB), D-16816 Neuruppin, Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Brosius
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000235:genetics*; D003712:Dendrites / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D009154:Mutation / Q000235:genetics; D009474:Neurons / Q000378:metabolism; D009690:Nucleic Acid Conformation; D059372:Nucleotide Motifs / Q000235:genetics*; D020733:RNA, Small Cytoplasmic / Q000378:metabolism*; D051381:Rats
chemical_list: C117734:BC1 RNA; D020733:RNA, Small Cytoplasmic