title: Cap mesenchyme cell swarming during kidney development is influenced by attraction, repulsion, and adhesion to the ureteric tip.
abstract: Morphogenesis of the mammalian kidney requires reciprocal interactions between two cellular domains at the periphery of the developing organ: the tips of the epithelial ureteric tree and adjacent regions of cap mesenchyme. While the presence of the cap mesenchyme is essential for ureteric branching, how it is specifically maintained at the tips is unclear. Using ex vivo timelapse imaging we show that cells of the cap mesenchyme are highly motile. Individual cap mesenchyme cells move within and between cap domains. They also attach and detach from the ureteric tip across time. Timelapse tracks collected for >800 cells showed evidence that this movement was largely stochastic, with cell autonomous migration influenced by opposing attractive, repulsive and cell adhesion cues. The resulting swarming behaviour maintains a distinct cap mesenchyme domain while facilitating dynamic remodelling in response to underlying changes in the tip.
authors.affiliation: Department of Anatomy & Neuroscience, University of Melbourne, Melbourne 3010 VIC, Australia; Murdoch Childrens Research Institute, Flemington Rd, Parkville, Melbourne, 3052 VIC, Australia. Electronic address: alexander.combes@unimelb.edu.au.
authors.forename: Alexander N
authors.initials: AN
authors.lastname: Combes
authors.affiliation: Division of Genomics of Development and Disease, Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: James G
authors.initials: JG
authors.lastname: Lefevre
authors.affiliation: Murdoch Childrens Research Institute, Flemington Rd, Parkville, Melbourne, 3052 VIC, Australia.
authors.forename: Sean
authors.initials: S
authors.lastname: Wilson
authors.affiliation: Division of Genomics of Development and Disease, Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia; Division of Cell Biology and Molecular Medicine, Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia.
authors.forename: Nicholas A
authors.initials: NA
authors.lastname: Hamilton
authors.affiliation: Murdoch Childrens Research Institute, Flemington Rd, Parkville, Melbourne, 3052 VIC, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, 3010 VIC, Australia. Electronic address: melissa.little@mcri.edu.au.
authors.forename: Melissa H
authors.initials: MH
authors.lastname: Little
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007668:Kidney / Q000166:cytology* / Q000196:embryology* / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D051379:Mice; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence; D009024:Morphogenesis / Q000502:physiology; D009924:Organ Culture Techniques; D013269:Stochastic Processes; D059008:Time-Lapse Imaging; D014513:Ureter / Q000166:cytology* / Q000196:embryology*
chemical_list: C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins
keywords: Cap mesenchyme; Cell migration; Kidney development; Live imaging; Nephron progenitor