title: Decreased expression of TLR7 in gastric cancer tissues and the effects of TLR7 activation on gastric cancer cells.
abstract: The present study aimed to determine the expression of Toll-like receptor 7 (TLR7) in gastric cancer tissues and investigate the effects of its activation on gastric cancer cells. Patients with gastric cancer (n=30) and patients without gastric cancer (control; n=14) who underwent gastroscopy were enrolled in the study. Gastric cancer and cancer-adjacent tissues were obtained from the patients with gastric cancer, and normal gastric epithelial tissues were obtained from the control patients. The TLR7 mRNA and protein expressions in different tissues were investigated by reverse transcription-quantitative polymerase chain reaction, western blotting and immunohistochemistry. The present study also determined the effects of TLR7 activation by the agonist imiquimod on TLR7 protein expression, proinflammatory cytokine secretion and viability in SGC-7901 gastric cancer cells. The mRNA and protein expression levels of TLR7 were significantly downregulated in gastric cancer tissues compared with cancer-adjacent and normal gastric epithelial tissues (P<0.01). Imiquimod significantly increased TLR7 protein expression levels, and promoted the secretion of proinflammatory cytokines tumor necrosis factor-α and interleukin-6 in SGC-7901 cells. Furthermore, imiquimod inhibited the proliferation of SGC-7901 cells in a dose- and time-dependent manner. Thus, the present study identified that the expression of TLR7 was decreased in gastric cancer tissues, and TLR7 activation enhanced TLR7 expression, promoted the production of proinflammatory cytokines and inhibited the growth of gastric cancer cells.
authors.affiliation: Department of Gastroenterology, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shanxi 710003, P.R. China.
authors.forename: Jiong
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Department of Gastroenterology, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shanxi 710003, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Dong
authors.affiliation: Department of Gastroenterology, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shanxi 710003, P.R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Qin
authors.affiliation: Department of Gastroenterology, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shanxi 710003, P.R. China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Shi
authors.affiliation: Department of Gastroenterology, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shanxi 710003, P.R. China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of Gastroenterology, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shanxi 710003, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
keywords: SGC-7901 cells; Toll-like receptor-7 expression; gastric cancer