title: Tracing the biotransformation of polychlorinated biphenyls (PCBs) in common carp (Cryprinus carpio): Enantiomeric fraction and compound-specific stable carbon isotope analyses.
abstract: Metabolites of polychlorinated biphenyls (PCBs) in fish are difficult to detect in vivo due to the complexity of biometabolism. In the present study, atropisomeric fraction analysis of chiral PCB congeners and compound-specific isotopic analysis (CSIA) were applied to trace the biotransformation of PCBs in fish by exposure of common carp (Cryprinus carpio) to the commercial PCB mixture Aroclor 1242. Stereoselective elimination of the chiral PCB congeners 91, 95, and 136 was observed, indicating a stereoselective biotransformation process. The δ(13)C values of PCBs 5/8, 18, and 20/33 in fish were increased compared with those in the spiked food, while PCBs 47/48 and 49 showed significant heavy isotope depletion. These results suggested a significant biotransformation of the corresponding individual PCB congeners although the potential PCB metabolites, hydroxylated PCBs (OH-PCBs) and methylsulfone PCBs (MeSO2-PCBs), were not detected in the fish tissue samples throughout this experiment. The results of the present study demonstrated that a combination of chiral analysis and CSIA is a promising new approach for investigating the biotransformation of PCBs in biota.
authors.affiliation: State Key Laboratory of Organic Geochemistry and Guangdong Key Laboratory of Environmental Resources Utilization and Protection, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, 510640, People's Republic of China; University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Organic Geochemistry and Guangdong Key Laboratory of Environmental Resources Utilization and Protection, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, 510640, People's Republic of China. Electronic address: luoxiaoj@gig.ac.cn.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Organic Geochemistry and Guangdong Key Laboratory of Environmental Resources Utilization and Protection, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, 510640, People's Republic of China.
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Zeng
authors.affiliation: State Key Laboratory of Organic Geochemistry and Guangdong Key Laboratory of Environmental Resources Utilization and Protection, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, 510640, People's Republic of China; University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China.
authors.forename: Run-Xia
authors.initials: RX
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Organic Geochemistry and Guangdong Key Laboratory of Environmental Resources Utilization and Protection, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, 510640, People's Republic of China; University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China.
authors.forename: Hua-Shan
authors.initials: HS
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Organic Geochemistry and Guangdong Key Laboratory of Environmental Resources Utilization and Protection, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, 510640, People's Republic of China; University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China.
authors.forename: Zong-Rui
authors.initials: ZR
authors.lastname: Li
authors.affiliation: State Key Laboratory of Organic Geochemistry and Guangdong Key Laboratory of Environmental Resources Utilization and Protection, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, 510640, People's Republic of China.
authors.forename: Bi-Xian
authors.initials: BX
authors.lastname: Mai
mesh_terms: D000818:Animals; D001711:Biotransformation; D002244:Carbon / Q000737:chemistry* / Q000378:metabolism*; D002247:Carbon Isotopes; D002347:Carps / Q000378:metabolism*; D006900:Hydroxylation; D011078:Polychlorinated Biphenyls / Q000378:metabolism* / Q000493:pharmacokinetics; D013237:Stereoisomerism
chemical_list: D002247:Carbon Isotopes; D002244:Carbon; D011078:Polychlorinated Biphenyls
keywords: Biotransformation; Compound-specific stable carbon isotope analysis (CSIA); Fish; Polychlorinated biphenyls (PCBs); Stereoselective