title: Chemical shift assignments and secondary structure prediction for Q4DY78, a conserved kinetoplastid-specific protein from Trypanosoma cruzi.
abstract: Trypanosoma cruzi, Trypanosma brucei and Leishmania spp. are kinetoplastid protozoa causative agents of Chagas disease, sleeping sickness and leishmaniasis, respectively, neglected tropical diseases estimated to infect millions of people worldwide. Their genome sequencing has revealed approximately 50 % of genes encoding hypothetical proteins of unknown function, opening possibilities for novel target identification and drug discovery. Q4DY78 is a putative essential protein from T. cruzi conserved in the related kinetoplastids and divergent from mammalian host proteins. Here we report the (1)H, (15)N, and (13)C chemical shift assignments and secondary structure analysis of the Q4DY78 protein as basis for NMR structure determination, functional analysis and drug screening.
authors.affiliation: Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho, 373 - Bloco E, sala 10, Rio de Janeiro, RJ, 21941-902, Brazil.
authors.forename: Éverton Dias
authors.initials: ÉD
authors.lastname: D'Andréa
authors.affiliation: Leibniz-Institut für Molekulare Pharmakologie, Robert-Rössle-Straße 10, 13125, Berlin, Germany.
authors.forename: Anne
authors.initials: A
authors.lastname: Diehl
authors.affiliation: Leibniz-Institut für Molekulare Pharmakologie, Robert-Rössle-Straße 10, 13125, Berlin, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Schmieder
authors.affiliation: Leibniz-Institut für Molekulare Pharmakologie, Robert-Rössle-Straße 10, 13125, Berlin, Germany.
authors.forename: Hartmut
authors.initials: H
authors.lastname: Oschkinat
authors.affiliation: Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho, 373 - Bloco E, sala 10, Rio de Janeiro, RJ, 21941-902, Brazil. jrmpires@cnrmn.bioqmed.ufrj.br.
authors.forename: José Ricardo
authors.initials: JR
authors.lastname: Pires
mesh_terms: D017124:Conserved Sequence; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D015800:Protozoan Proteins / Q000737:chemistry*; D014349:Trypanosoma cruzi
chemical_list: D015800:Protozoan Proteins
keywords: Hypothetical protein Q4DY78; Kinetoplastids; NMR assignments; Neglected diseases; Trypanosoma cruzi