title: Targeted treatment of liver metastasis from gastric cancer using specific binding peptide.
abstract: Gastric cancer ranks the first in China among all gastrointestinal cancers in terms of incidence, and liver metastasis is the leading cause of death for patients with advanced gastric cancer. Tumor necrosis factor (TNF) is a cytokine commonly chosen as the target for gene therapy against cancers. The specific binding peptide pd20 of gastric cancer cells with a high potential for liver metastasis was fused with human TNF to obtain the pd20-TNF gene using DNA recombinant technique. The expression of the fusion protein was induced and the protein was purified. In vitro activity test showed that the fusion protein greatly improved the membrane permeability of liver cells in nude mice with liver metastasis from gastric cancer. The tumor implantation experiment in nude mice showed that the fusion protein effectively mitigated the cancer lesions. The results provide important clues for developing the drugs for targeted treatment of liver metastasis from gastric cancer.
authors.affiliation: Department of General Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital No. 600, Yishan Road, Shanghai 200233, P. R. China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Gong
authors.affiliation: Department of General Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital No. 600, Yishan Road, Shanghai 200233, P. R. China.
authors.forename: Gewen
authors.initials: G
authors.lastname: Tan
authors.affiliation: Department of General Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital No. 600, Yishan Road, Shanghai 200233, P. R. China.
authors.forename: Nengquan
authors.initials: N
authors.lastname: Sheng
authors.affiliation: Department of General Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital No. 600, Yishan Road, Shanghai 200233, P. R. China.
authors.forename: Weiqiang
authors.initials: W
authors.lastname: You
authors.affiliation: Department of General Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital No. 600, Yishan Road, Shanghai 200233, P. R. China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Wang
keywords: Liver metastasis from gastric cancer; specific binding peptide; targeted treatment