title: An Effective New Cryopreservation Procedure for Pancreatic Islets Using Hollow Fiber Vitrification.
abstract: The present study aimed at establishing a new cryopreservation method for mouse pancreatic islets by vitrification using hollow fibers as a container. A unique feature of the hollow fiber vitrification (HFV) method is that this method achieves stable vitrification using a minimum volume of cryoprotectant (CPA) solution, thereby ensuring high viability of the islets. The cytotoxicity, optimum composition, and concentration of the CPAs for vitrifying islets were examined. The viability, functional-integrity of vitrified islets were evaluated in comparison with those vitrified by conventional methods. Insulin secretion was measured in vitro by a static incubation assay and the metabolic functions was tested after transplantation into Streptozotocin-induced diabetic mice. The combination of 15% dimethyl sulfoxide+15% ethylene glycol resulted in the best CPA solution for the HFV of islets. HFV showed the highest viability in comparison to 2 vitrification methods, open pulled straws and vitrification with EDT324 solution. The vitrified islets stably expressed β-cells markers NeuroD, Pancreatic and duodenal homeobox-1, and MafA. Transplantation of the vitrified islets achieved euglycemia of the host diabetic mice and response to an intraperitoneal glucose tolerance test to a similar extent as non-vitrified transplanted islets. The HFV method allows for efficient long-term cryopreservation of islets.
authors.affiliation: Meiji University International Institute for Bio-Resource Research, Higashimita Tama-ku Kawasaki Kanagawa, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Nagaya
authors.affiliation: Meiji University International Institute for Bio-Resource Research, Higashimita Tama-ku Kawasaki Kanagawa, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Matsunari
authors.affiliation: Laboratory of Developmental Engineering, Department of Life Sciences, School of Agriculture, Meiji University, Higashimita Tama-ku Kawasaki Kanagawa, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kanai
authors.affiliation: Laboratory of Developmental Engineering, Department of Life Sciences, School of Agriculture, Meiji University, Higashimita Tama-ku Kawasaki Kanagawa, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Maehara
authors.affiliation: Meiji University International Institute for Bio-Resource Research, Higashimita Tama-ku Kawasaki Kanagawa, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nakano
authors.affiliation: Laboratory of Developmental Engineering, Department of Life Sciences, School of Agriculture, Meiji University, Higashimita Tama-ku Kawasaki Kanagawa, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Umeki
authors.affiliation: Laboratory of Developmental Engineering, Department of Life Sciences, School of Agriculture, Meiji University, Higashimita Tama-ku Kawasaki Kanagawa, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Katsumata
authors.affiliation: Laboratory of Developmental Engineering, Department of Life Sciences, School of Agriculture, Meiji University, Higashimita Tama-ku Kawasaki Kanagawa, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kasai
authors.affiliation: Laboratory of Developmental Engineering, Department of Life Sciences, School of Agriculture, Meiji University, Higashimita Tama-ku Kawasaki Kanagawa, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Sakai
authors.affiliation: Laboratory of Developmental Engineering, Department of Life Sciences, School of Agriculture, Meiji University, Higashimita Tama-ku Kawasaki Kanagawa, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kobayashi
authors.affiliation: Meiji University International Institute for Bio-Resource Research, Higashimita Tama-ku Kawasaki Kanagawa, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Honda
authors.affiliation: Laboratory of Developmental Engineering, Department of Life Sciences, School of Agriculture, Meiji University, Higashimita Tama-ku Kawasaki Kanagawa, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Abe
authors.affiliation: Meiji University International Institute for Bio-Resource Research, Higashimita Tama-ku Kawasaki Kanagawa, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Watanabe
authors.affiliation: Meiji University International Institute for Bio-Resource Research, Higashimita Tama-ku Kawasaki Kanagawa, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Umeyama
authors.affiliation: Meiji University International Institute for Bio-Resource Research, Higashimita Tama-ku Kawasaki Kanagawa, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Nagashima
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D007515:Islets of Langerhans / Q000187:drug effects / Q000502:physiology*; D016381:Islets of Langerhans Transplantation; D008297:Male; D008813:Mice, Inbred ICR; D016513:Mice, SCID; D009994:Osmolar Concentration; D012996:Solutions; D013696:Temperature; D014022:Tissue Survival / Q000187:drug effects; D058989:Vitrification / Q000187:drug effects
chemical_list: D003451:Cryoprotective Agents; D012996:Solutions