title: Integration-free T cell-derived human induced pluripotent stem cells (iPSCs) from a patient with lymphedema-distichiasis syndrome (LDS) carrying an insertion-deletion complex mutation in the FOXC2 gene.
abstract: Expanded human T cells from a Japanese male with lymphedema-distichiasis syndrome (LDS) were used to generate integration-free induced pluripotent stem cells (iPSCs) by exogenous expression of four reprogramming factors, OCT3/4, SOX2, cMYC, KLF4, using Sendai virus vector (SeVdp). The authenticity of established iPSC line, LDS-iPSC8, was confirmed by the expression of stem cell markers and the differentiation capability into three germ layers. LDS-iPSC8 may be a useful cell resource for the establishment of in vitro LDS modeling and the study for vascular and lymph vessel development.
authors.affiliation: Department of Dermatology, The Jikei University School of Medicine, Tokyo, Japan. Electronic address: seafowl@jikei.ac.jp.
authors.forename: Munenari
authors.initials: M
authors.lastname: Itoh
authors.affiliation: Department of Dermatology, The Jikei University School of Medicine, Tokyo, Japan.
authors.forename: Shiho
authors.initials: S
authors.lastname: Kawagoe
authors.affiliation: Division of Regenerative Medicine, The Jikei University School of Medicine, Tokyo, Japan.
authors.forename: Hirotaka James
authors.initials: HJ
authors.lastname: Okano
authors.affiliation: Department of Dermatology, The Jikei University School of Medicine, Tokyo, Japan.
authors.forename: Hidemi
authors.initials: H
authors.lastname: Nakagawa
mesh_terms: D000293:Adolescent; D001483:Base Sequence; D002454:Cell Differentiation; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D019175:DNA Methylation; D004252:DNA Mutational Analysis; D058732:Embryoid Bodies / Q000166:cytology / Q000378:metabolism; D005140:Eyelashes / Q000002:abnormalities* / Q000473:pathology; D051858:Forkhead Transcription Factors / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D054643:INDEL Mutation; D057026:Induced Pluripotent Stem Cells / Q000166:cytology; D059785:Karyotype; D000090062:Kruppel-Like Factor 4; D008209:Lymphedema / Q000235:genetics* / Q000473:pathology; D008297:Male; D029082:Sendai virus / Q000235:genetics; D013601:T-Lymphocytes / Q000166:cytology; D013724:Teratoma / Q000378:metabolism / Q000473:pathology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D051858:Forkhead Transcription Factors; C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4; D014157:Transcription Factors; C082078:mesenchyme fork head 1 protein