title: In vitro synergistic combinations of pentamidine, polymyxin B, tigecycline and tobramycin with antifungal agents against Fusarium spp.
abstract: The genus Fusarium is characterized by hyaline filamentous fungi that cause infections predominantly in immunocompromised patients. The remarkable primary resistance to antifungal agents of this genus requires a search for new therapeutic possibilities. This study assessed the in vitro susceptibility of 25 clinical isolates of Fusarium against antifungal agents (amphotericin B, caspofungin, itraconazole and voriconazole) and antimicrobials (pentamidine, polymyxin B, tigecycline and tobramycin) according to the broth microdilution method (M38-A2). The interactions between antifungal and antimicrobial agents were evaluated by the microdilution checkerboard method. Pentamidine and polymyxin B showed MIC values ≥4 µg ml-1 against Fusarium spp. The highest rates of synergism were observed when amphotericin B or voriconazole was combined with tobramycin (80 % and 76 %, respectively), polymyxin B (76 % and 64 %) and pentamidine (72 % and 68 %). The most significant combinations deserve in vivo evaluations in order to verify their potential in the treatment of fusariosis.
authors.affiliation: Postgraduate Program in Pharmaceutical Sciences, Health Sciences Center, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Tarcieli
authors.initials: T
authors.lastname: Pozzebon Venturini
authors.affiliation: Postgraduate Program in Pharmaceutical Sciences, Health Sciences Center, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Luana
authors.initials: L
authors.lastname: Rossato
authors.affiliation: Postgraduate Program in Pharmaceutical Sciences, Health Sciences Center, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Francieli
authors.initials: F
authors.lastname: Chassot
authors.affiliation: Department of Microbiology and Parasitology, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Jéssica
authors.initials: J
authors.lastname: Tairine Keller
authors.affiliation: Postgraduate Program in Pharmaceutical Sciences, Health Sciences Center, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Baldissera Piasentin
authors.affiliation: Department of Microbiology and Parasitology, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.|Postgraduate Program in Pharmacology, Health Sciences Center, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Janio
authors.initials: J
authors.lastname: Morais Santurio
authors.affiliation: Postgraduate Program in Pharmaceutical Sciences, Health Sciences Center, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.|Department of Microbiology and Parasitology, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Sydney
authors.initials: S
authors.lastname: Hartz Alves
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D004357:Drug Synergism; D005670:Fusarium / Q000187:drug effects*; D006801:Humans; D008826:Microbial Sensitivity Tests; D010419:Pentamidine / Q000494:pharmacology*
chemical_list: D000890:Anti-Infective Agents; D010419:Pentamidine