title: IR and Raman imaging of murine brains from control and ApoE/LDLR(-/-) mice with advanced atherosclerosis.
abstract: Confocal Raman mapping and FT-IR imaging combined with chemometric analysis was used to study the alterations in murine brain tissue induced by the development of atherosclerosis. FT-IR imaging allowed us to obtain lower spatial resolution data (∼5.5 μm) from large, representative cross-sectional brain areas, while Raman mapping provided a more detailed insight into chosen regions of interest with high spatial resolution (∼0.4 μm). A comparison of white (WM) and grey matter (GM) from control (C57BL/6J) and ApoE/LDLR(-/-) mice with advanced atherosclerosis revealed disease-induced changes in both: GM and WM. The alterations included an increased lipid to protein ratio and higher total content of cholesterol.
authors.affiliation: Jagiellonian University, Jagiellonian Centre for Experimental Therapeutics (JCET), 14 Bobrzyńskiego Str., 30-348 Krakow, Poland. katarzyna.marzec@jcet.eu marzec@chemia.uj.edu.pl.
authors.forename: Kamila
authors.initials: K
authors.lastname: Kochan
authors.forename: Karolina
authors.initials: K
authors.lastname: Chrabaszcz
authors.forename: Barbara
authors.initials: B
authors.lastname: Szczur
authors.forename: Edyta
authors.initials: E
authors.lastname: Maslak
authors.forename: Jakub
authors.initials: J
authors.lastname: Dybas
authors.forename: Katarzyna M
authors.initials: KM
authors.lastname: Marzec
mesh_terms: D000818:Animals; D050197:Atherosclerosis / Q000000981:diagnostic imaging*; D001921:Brain / Q000000981:diagnostic imaging*; D051379:Mice; D008810:Mice, Inbred C57BL; D000074085:Mice, Knockout, ApoE; D017550:Spectroscopy, Fourier Transform Infrared; D013059:Spectrum Analysis, Raman