title: Inhibitory effect of aminoethyl-chitooligosaccharides on invasion of human fibrosarcoma cells.
abstract: Chitooligosaccharides (COS) have been reported to show a variety of biological efficacies such as anti-bacterial activity, anti-tumor activity and immune activity. The purpose of this study is to investigate the inhibitory effect of aminoethyl-chitooligosaccharides (AE-COS) synthesized from COS that were substituted hydroxyl groups with aminoethyl group at C-6 position on cell invasion of human fibrosarcoma cells. First of all, the effect of AE-COS on cell viability was observed using MTT assay. The cytotoxicity of AE-COS was increased in a dose dependent manner. The inhibitory effects of AE-COS on the activity and expression level of MMP-2 and MMP-9 related to invasion of cancer cells were examined using gelatin zymography and western blot. It was found that AE-COS above 20μg/ml showed the inhibitory effect on the activity and expression of MMP-9. Furthermore, AE-COS at 20μg/ml reduced the expression level of p50, a part of NF-κB, compared with phorbol-12- myristate-13- acetate (PMA) group. The available data let us hypothesize that AE-COS could provide chemoprevention as an inhibitor against cell invasion associated with metastasis.
authors.affiliation: Department of Chemistry, Dong-Eui University, Busan 614-714, Republic of Korea.
authors.forename: Sugyeong
authors.initials: S
authors.lastname: Hong
authors.affiliation: Department of Biochemistry, Faculty of Biology, University of Science, VNU-HCM, Ho Chi Minh City, Viet Nam, Viet Nam.
authors.forename: Dai-Nghiep
authors.initials: DN
authors.lastname: Ngo
authors.affiliation: Department of Chemistry, Dong-Eui University, Busan 614-714, Republic of Korea. Electronic address: mmkim@deu.ac.kr.
authors.forename: Moon-Moo
authors.initials: MM
authors.lastname: Kim
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D001681:Biological Assay; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D048271:Chitosan; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D061965:Matrix Metalloproteinase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000235:genetics; D009844:Oligosaccharides
chemical_list: D000970:Antineoplastic Agents; D061965:Matrix Metalloproteinase Inhibitors; D016328:NF-kappa B; D009844:Oligosaccharides; C493484:oligochitosan; D002686:Chitin; D048271:Chitosan; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9
keywords: AE-COS; Immunofluorescence; Invasion; MMP-9; NF-κB