title: Adipose-derived stem cells sustain prolonged angiogenesis through leptin secretion.
abstract: Recent studies suggest that adipose-derived stem cells (ASCs) play a role in tissue remodeling through the release of cytokines and growth factors. We compared the secreted cytokine profile of hypoxia-conditioned ASCs (hASCs) with normoxic ASCs (nASCs) and we analyzed the effect of ASCs conditioned medium (CM) on endothelial cells. We found that hypoxia induced a transient upregulation of VEGF in ASCs and a notable and enduring upregulation of leptin mRNA expression 30-fold greater than control after 24 h and up to 60-fold greater than control at day 7. CM from hASC stimulated EC tube formation to a significantly greater extent than CM from nASC. This might be due to leptin-secreted factor. Indeed, exogenous leptin stimulated the expression of HIF2-α, but not HIF1-α, and upregulated the expression of Flt-1 and Tie-1 proangiogenic receptors. In conclusion, hASCs may be particularly efficient in sustaining angiogenesis through the release of leptin.
authors.affiliation: a Department of Biotechnological and Applied Clinical Sciences and.
authors.forename: Simona
authors.initials: S
authors.lastname: Delle Monache
authors.affiliation: a Department of Biotechnological and Applied Clinical Sciences and.
authors.forename: Alessia
authors.initials: A
authors.lastname: Calgani
authors.affiliation: a Department of Biotechnological and Applied Clinical Sciences and.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Sanità
authors.affiliation: a Department of Biotechnological and Applied Clinical Sciences and.
authors.forename: Francesca
authors.initials: F
authors.lastname: Zazzeroni
authors.affiliation: a Department of Biotechnological and Applied Clinical Sciences and.|b Division of Surgery , University of L'Aquila , L'Aquila , Italy.
authors.forename: Emilio
authors.initials: E
authors.lastname: Gentile Warschauer
authors.affiliation: a Department of Biotechnological and Applied Clinical Sciences and.|b Division of Surgery , University of L'Aquila , L'Aquila , Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Giuliani
authors.affiliation: a Department of Biotechnological and Applied Clinical Sciences and.|b Division of Surgery , University of L'Aquila , L'Aquila , Italy.
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Amicucci
authors.affiliation: a Department of Biotechnological and Applied Clinical Sciences and.
authors.forename: Adriano
authors.initials: A
authors.lastname: Angelucci
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000378:metabolism; D000328:Adult; D053687:Adult Stem Cells / Q000378:metabolism*; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D015687:Cell Hypoxia; D017077:Culture Media, Conditioned / Q000494:pharmacology; D042783:Endothelial Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism; D020738:Leptin / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D018919:Neovascularization, Physiologic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000099162:Endothelial PAS Domain-Containing Protein 1
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D017077:Culture Media, Conditioned; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D020738:Leptin; D012333:RNA, Messenger; D000099162:Endothelial PAS Domain-Containing Protein 1; C497442:HIF1A protein, human
keywords: Angiogenesis; CD105; endothelial progenitor cells; growth factors