title: ICP35 Is a TREX-Like Protein Identified in White Spot Syndrome Virus.
abstract: ICP35 is a non-structural protein from White spot syndrome virus believed to be important in viral replication. Since ICP35 was found to localize in the host nucleus, it has been speculated that the function of ICP35 might be involved in the interaction of DNA. In this study, we overexpressed, purified and characterized ICP35. The thioredoxin-fused ICP35 (thio-ICP35) was strongly expressed in E. coli and be able to form itself into dimers. Investigation of the interaction between ICP35 and DNA revealed that ICP35 can perform DNase activity. Structural model of ICP35 was successfully built on TREX1, suggesting that ICP35 might adopt the folding similar to that of TREX1 protein. Several residues important for dimerization in TREX1 are also conserved in ICP35. Residue Asn126 and Asp132, which are seen to be in close proximity to metal ions in the ICP35 model, were shown through site-directed mutagenesis to be critical for DNase activity.
authors.affiliation: Department of Microbiology, Faculty of Science, King Mongkut's University of Technology Thonburi, Bangmod, Bangkok, 10140, Thailand.
authors.forename: Panapat
authors.initials: P
authors.lastname: Phairoh
authors.affiliation: Department of Physics, Faculty of Science, King Mongkut's University of Technology Thonburi, Bangmod, Bangkok, 10140, Thailand.
authors.forename: Thana
authors.initials: T
authors.lastname: Suthibatpong
authors.affiliation: Department of Microbiology, Faculty of Science, King Mongkut's University of Technology Thonburi, Bangmod, Bangkok, 10140, Thailand.
authors.forename: Triwit
authors.initials: T
authors.lastname: Rattanarojpong
authors.affiliation: Department of Microbiology, Faculty of Science, King Mongkut's University of Technology Thonburi, Bangmod, Bangkok, 10140, Thailand.
authors.forename: Nujarin
authors.initials: N
authors.lastname: Jongruja
authors.affiliation: National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani, 12120, Thailand.|Center of Excellence for Shrimp Molecular Biology and Biotechnology, Mahidol University, 272 Rama VI Road, Bangkok, 10400, Thailand.
authors.forename: Saengchan
authors.initials: S
authors.lastname: Senapin
authors.affiliation: Department of Biochemistry Faculty of Science Kasetsart University, Bangkok, 10900, Thailand.
authors.forename: Kiattawee
authors.initials: K
authors.lastname: Choowongkomon
authors.affiliation: Department of Microbiology, Faculty of Science, King Mongkut's University of Technology Thonburi, Bangmod, Bangkok, 10140, Thailand.
authors.forename: Pongsak
authors.initials: P
authors.lastname: Khunrae
mesh_terms: D003001:Cloning, Molecular; D004789:Enzyme Activation; D005090:Exodeoxyribonucleases; D015870:Gene Expression; D006860:Hydrogen Bonding; D008958:Models, Molecular; D016296:Mutagenesis; D010750:Phosphoproteins; D011487:Protein Conformation; D055503:Protein Multimerization; D011994:Recombinant Proteins; D017361:Viral Nonstructural Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D046848:White spot syndrome virus 1 / Q000235:genetics* / Q000378:metabolism
chemical_list: D010750:Phosphoproteins; D011994:Recombinant Proteins; D017361:Viral Nonstructural Proteins; D005090:Exodeoxyribonucleases; C429526:three prime repair exonuclease 1