title: Pre-Multidrug-Resistant Mycobacterium tuberculosis Infection Causing Fatal Enteric Disease in a Dog from a Family with History of Human Tuberculosis.
abstract: This report describes a fatal case of a pet dog with major enteric signs owned by a family that has experienced cases of pulmonary tuberculosis (TB) in the household. Clinical and epidemiological aspects, imaging data, microbiological, haematological and histopathological examinations were assessed to diagnosis of disease. gyrB-RFLP, spoligotyping and MIRU-VNTR allowed molecular detection of M. tuberculosis strain from S family. The resazurin microtiter assay indicated that all isolates were resistant to isoniazid, ethambutol, ciprofloxacin, ofloxacin, streptomycin and amikacin. The public health concerns related to canine tuberculosis and risk of the dissemination by pets of M. tuberculosis pre-multidrug-resistant (PMD) to isoniazid, ethambutol and other first-line drugs used in human therapy of TB are discussed. We believe this to be the first report of PMD M. tuberculosis infection in a dog presenting mainly enteric manifestation, confirmed as S lineage by molecular methods, owned by a family in which TB has spread in the household for generations.
authors.affiliation: UNESP-Univ. Estadual Paulista, Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Sciences, Botucatu, State of São Paulo, Brazil.
authors.forename: M G
authors.initials: MG
authors.lastname: Ribeiro
authors.affiliation: UNESP-Univ. Estadual Paulista, Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Sciences, Botucatu, State of São Paulo, Brazil.
authors.forename: M C F
authors.initials: MCF
authors.lastname: Lima
authors.affiliation: UNESP-Univ. Estadual Paulista, Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Sciences, Botucatu, State of São Paulo, Brazil.
authors.forename: M M J
authors.initials: MMJ
authors.lastname: Franco
authors.affiliation: UNESP-Univ. Estadual Paulista, Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Sciences, Botucatu, State of São Paulo, Brazil.
authors.forename: J
authors.initials: J
authors.lastname: Megid
authors.affiliation: UNESP-Univ. Estadual Paulista, Department of Clinics, School of Veterinary Medicine and Animal Sciences, Botucatu, State of São Paulo, Brazil.
authors.forename: L M
authors.initials: LM
authors.lastname: Soares
authors.affiliation: UNESP-Univ. Estadual Paulista, Department of Clinics, School of Veterinary Medicine and Animal Sciences, Botucatu, State of São Paulo, Brazil.
authors.forename: L H A
authors.initials: LHA
authors.lastname: Machado
authors.affiliation: Laboratory of Micobacteriology, Faculty of Pharmacy, UNESP, Araraquara, State of São Paulo, Brazil.
authors.forename: M
authors.initials: M
authors.lastname: Miyata
authors.affiliation: Laboratory of Micobacteriology, Faculty of Pharmacy, UNESP, Araraquara, State of São Paulo, Brazil.
authors.forename: F R
authors.initials: FR
authors.lastname: Pavan
authors.affiliation: Department of Preventive Veterinary Medicine and Animal Health, University of São Paulo, São Paulo, State of São Paulo, Brazil.
authors.forename: M B
authors.initials: MB
authors.lastname: Heinemann
authors.affiliation: Department of Preventive Veterinary Medicine and Animal Health, University of São Paulo, São Paulo, State of São Paulo, Brazil.
authors.forename: A F
authors.initials: AF
authors.lastname: Souza Filho
authors.affiliation: UNESP-Univ. Estadual Paulista, Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Sciences, Botucatu, State of São Paulo, Brazil.
authors.forename: G H B
authors.initials: GHB
authors.lastname: Lara
authors.affiliation: UNISA, University of Santo Amaro, São Paulo, State of São Paulo, Brazil.
authors.forename: O C
authors.initials: OC
authors.lastname: Sanches
authors.affiliation: UNESP-Univ. Estadual Paulista, Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Sciences, Botucatu, State of São Paulo, Brazil.
authors.forename: C D C
authors.initials: CDC
authors.lastname: Sanches
authors.affiliation: UNESP-Univ. Estadual Paulista, Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Sciences, Botucatu, State of São Paulo, Brazil.
authors.forename: F J P
authors.initials: FJP
authors.lastname: Listoni
authors.affiliation: UNESP-Univ. Estadual Paulista, Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Sciences, Botucatu, State of São Paulo, Brazil.
authors.forename: A C
authors.initials: AC
authors.lastname: Paes
mesh_terms: D000818:Animals; D000995:Antitubercular Agents / Q000494:pharmacology; D004283:Dog Diseases / Q000175:diagnosis / Q000382:microbiology*; D004285:Dogs; D024901:Drug Resistance, Multiple, Bacterial; D004751:Enteritis / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D017809:Fatal Outcome; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology; D008297:Male; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000302:isolation & purification; D057805:Pets; D018088:Tuberculosis, Multidrug-Resistant; D014397:Tuberculosis, Pulmonary / Q000382:microbiology*
chemical_list: D000995:Antitubercular Agents; D007538:Isoniazid
keywords: S family mycobacteria; anthropozoonosis; canine tuberculosis; enteric manifestation; multidrug resistance