title: Long form collapsin response mediator protein-1 promotes the migration and invasion of osteosarcoma cells.
abstract: It has been reported that long form collapsin response mediator protein-1 (LCRMP-1) promotes the metastasis of non-small cell lung cancer. Osteosarcoma (OS) is a human cancer with a high potential for metastasis. The present study aimed to investigate the role of LCRMP-1 in OS metastasis. The expression of LCRMP-1 in OS specimens and cell lines was evaluated using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blot analysis. Furthermore, the migration and invasion of OS cells with LCRMP-1-knockdown was investigated to examine the role of LCRMP-1 in OS metastasis. In addition, the expression of N-cadherin and matrix metalloproteinases (MMPs), which are involved in cell migration, was evaluated using RT-qPCR. Increased expression of LCRMP-1 was observed in the OS tissues and cell lines, accompanied by the enhanced migration and invasion of the OS cells. LCRMP-1-knockdown resulted in a significant decrease in the expression of N-cadherin and MMPs, as well as inhibition of the migration and invasion of the OS cells. Overexpression of LCRMP-1 promoted OS metastasis. Therefore, LCRMP-1 may be a promising target for the effective treatment of OS.
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong 510632, P.R. China.
authors.forename: Huige
authors.initials: H
authors.lastname: Hou
authors.affiliation: College of Pharmacy, Jinan University, Guangzhou, Guangdong 510632, P.R. China; Department of Pharmacy, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong 510632, P.R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong 510632, P.R. China.
authors.forename: Zhengang
authors.initials: Z
authors.lastname: Zha
authors.affiliation: College of Pharmacy, Jinan University, Guangzhou, Guangdong 510632, P.R. China.
authors.forename: Shaohui
authors.initials: S
authors.lastname: Cai
authors.affiliation: School of Medicine, Jinan University, Guangzhou, Guangdong 510632, P.R. China.
authors.forename: Minghui
authors.initials: M
authors.lastname: Tan
authors.affiliation: School of Medicine, Jinan University, Guangzhou, Guangdong 510632, P.R. China.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Guo
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong 510632, P.R. China.
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong 510632, P.R. China.
authors.forename: Guorong
authors.initials: G
authors.lastname: She
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong 510632, P.R. China.
authors.forename: Songwei
authors.initials: S
authors.lastname: Xun
keywords: invasion; long form collapsin response mediator protein-1; migration; osteosarcoma