title: Molecular characterisation of epizootic haemorrhagic disease virus associated with a Tunisian outbreak among cattle in 2006.
abstract: In 2006, epizootic haemorrhagic disease (EHD) outbreaks were recorded in the Maghreb (Tunisia, Morocco and Algeria) among cattle, resulting in severe repercussions on herds (oedema of the head, necrotic lesions of the oral mucosa, hyperthermia of the teats, accompanied by anorexia and respiratory distress) and economic losses. The present study gives new information on the molecular characterisation of the EHD virus (EHDV) that had circulated in Tunisia. Genome segments 2, 3, 6, 7 and 10 of EHDV, corresponding to the VP2, VP3, VP5, VP7 and NS3/NS3A proteins, respectively, were amplified from the blood of one animal by RT-PCR and sequenced. Nucleotide sequence comparisons of these five segments with sequences available in the GenBank demonstrated that an EHDV serotype 6 (EHDV-6) had been present in Tunisia in 2006. The possible origin of this strain is discussed.
authors.affiliation: Anses, INRA, ENVA, UPEC, UMR 1161 Virology , 23 avenue du général du Gaulle, 94706 Maisons-Alfort , France.|IRVT , Tunis-La Rabta , Tunisia.|Faculty of Sciences of Bizerte , Jarzouna Bizerte , Tunisia - under the tutelage of Carthage University, Tunisia.
authors.forename: Sameh
authors.initials: S
authors.lastname: Ben Dhaou
authors.affiliation: Anses, INRA, ENVA, UPEC, UMR 1161 Virology , 23 avenue du général du Gaulle, 94706 Maisons-Alfort , France.
authors.forename: Corinne
authors.initials: C
authors.lastname: Sailleau
authors.affiliation: IRVT , Tunis-La Rabta , Tunisia.
authors.forename: Besma
authors.initials: B
authors.lastname: Babay
authors.affiliation: Anses, INRA, ENVA, UPEC, UMR 1161 Virology , 23 avenue du général du Gaulle, 94706 Maisons-Alfort , France.
authors.forename: Cyril
authors.initials: C
authors.lastname: Viarouge
authors.affiliation: IRVT , Tunis-La Rabta , Tunisia.
authors.forename: Soufien
authors.initials: S
authors.lastname: Sghaier
authors.affiliation: Anses, INRA, ENVA, UPEC, UMR 1161 Virology , 23 avenue du général du Gaulle, 94706 Maisons-Alfort , France.
authors.forename: Stephan
authors.initials: S
authors.lastname: Zientara
authors.affiliation: IRVT , Tunis-La Rabta , Tunisia.|ENMV , Sidi Thabet, Tunis , Tunisia - under the tutelage of Manouba University, Tunisia.
authors.forename: Salah
authors.initials: S
authors.lastname: Hammami
authors.affiliation: Anses, INRA, ENVA, UPEC, UMR 1161 Virology , 23 avenue du général du Gaulle, 94706 Maisons-Alfort , France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Bréard
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000821:virology*; D004196:Disease Outbreaks / Q000662:veterinary*; D018164:Hemorrhagic Disease Virus, Epizootic / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D012088:Reoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D014416:Tunisia / Q000453:epidemiology
keywords: Epizootic haemorrhagic disease virus; RT-PCR; phylogenetic tree; sequencing; serotype 6