title: [Effects of Germanium Concentrations on Germanium Accumulation and Biotransformation of Polysaccarified Germanium in Cordyceps militaris].
abstract: OBJECTIVE
  To study the effects of Germanium (Ge) concentration on Ge accumulation and biotransformation of polysaccarified Ge (PG) in Cordyceps militaris.
  
  
  METHODS
  Solid and liquid culture were used in this study.
  
  
  RESULTS
  In the solid culture conditions, when the Ge concentration of medium was 200 mg/L, the sporophore biomass of Cordyceps militaris was the maximum; and when Ge concentration was 300 mg/L,the amount of biotransformation of PG in sporophore was the highest; and when the Ge concentration is 250 mg/L, conversion rate of organic germanium (OG) in sporophore reached the highest value. In the liquid culture conditions, when the Ge concentration was 250 mg/L, the mycelium biomass of Cordyceps militaris was the maximum; and when Ge concentration was 150 mg/L, the amount of organic conversion of PG in mycelium was the most; and conversion rate of OG in mycelium was the highest in media with the Ge concentration of 200 mg/L. This study showed the germanium concentrations in 150 - 300 mg/L was more suitable for Ge accumulation and biotransformation of PG in Cordyceps militaris. In general, the biotransformation capacity to germanium of sporophore was stronger than that of mycelium of Cordyceps militaris.
  
  
  CONCLUSIONS
  Germanium can significantly affect Ge accumulation and biotransformation of PG in Cordyceps militaris (P < 0.05) at different concentration. This result has practical value for Ge enriched cultivation of fruiting body in Cordyceps militaris.
authors.forename: Ju-feng
authors.initials: JF
authors.lastname: Wang
authors.forename: Hu-ming
authors.initials: HM
authors.lastname: Li
authors.forename: Dao-de
authors.initials: DD
authors.lastname: Yang
mesh_terms: D018533:Biomass; D001711:Biotransformation; D032661:Cordyceps; D003470:Culture Media / Q000737:chemistry*; D005857:Germanium / Q000378:metabolism*; D025282:Mycelium
chemical_list: D003470:Culture Media; D005857:Germanium