title: Preparation of amino acid-based polymer functionalized magnetic nanoparticles as adsorbents for analysis of plant growth regulators in bean sprouts.
abstract: A novel magnetic solid phase extraction (MSPE) adsorbent has been developed for enriching two plant growth regulators, including 2,4-dichlorophenoxyacetic acid (2,4-D) and 4-chlorophenoxyacetic acid (4-CPA), in bean sprouts. For preparing the MSPE adsorbent, poly(N-methacryloyl-L-phenylalanine methyl ester (P(MA-L-Phe-OMe)), amino acid-based polymer, was modified onto the magnetic nanoparticles via "grafting to" method by free radical polymerization. The resultant P(MA-L-Phe-OMe)-functionalized magnetic nanoparticles (Fe3O4@P(MA-L-Phe-OMe)) were characterized by Fourier transform infrared (FT-IR) spectroscopy and elemental analysis. The adsorption amount of Fe3O4@P(MA-L-Phe-OMe) nanoparticles to 2,4-D and 4-CPA were 39.82mgg(-1) and 29.02mgg(-1), respectively. Moreover, the prepared MSPE adsorbents showed good selectivity towards 2,4-D and 4-CPA due to the hydrophobic interactions and electrostatic forces between the target analytes and Fe3O4@P(MA-L-Phe-OMe). The results demonstrated that the proposed MSPE adsorbents have high affinity to the targets 2,4-D and 4-CPA. Under the optimized conditions, the proposed materials were successfully applied to enrich 2,4-D and 4-CPA in bean sprouts samples. The recovery values of the bean sprouts solution spiked the targets were from 90.9% to 96.4% with the relative standard deviations of 2.3-3.9%. Our work proved that the novel Fe3O4@P(MA-L-Phe-OMe) nanoparticles were the good adsorbents of magnetic solid phase extraction (MSPE) and have good potential for the analysis of trace compound in real samples.
authors.affiliation: Beijing National Laboratory of Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, No. 2 Zhongguancun Beiyijie, Beijing 100190, China; College of Food Sciences and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, China.
authors.forename: Shilei
authors.initials: S
authors.lastname: Ji
authors.affiliation: Beijing National Laboratory of Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, No. 2 Zhongguancun Beiyijie, Beijing 100190, China; University of Chinese Academy of Sciences, 19A Yuquanlu, Beijing 100049, China. Electronic address: qili@iccas.ac.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Qi
authors.affiliation: Beijing National Laboratory of Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, No. 2 Zhongguancun Beiyijie, Beijing 100190, China; University of Chinese Academy of Sciences, 19A Yuquanlu, Beijing 100049, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.affiliation: College of Food Sciences and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, China.
authors.forename: Minglin
authors.initials: M
authors.lastname: Wang
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000031:analogs & derivatives* / Q000032:analysis* / Q000737:chemistry; D000327:Adsorption; D007887:Fabaceae; D060328:Magnetic Phenomena; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D010649:Phenylalanine / Q000031:analogs & derivatives* / Q000737:chemistry; D010937:Plant Growth Regulators / Q000032:analysis* / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000737:chemistry*; D036226:Seedlings / Q000737:chemistry*; D052616:Solid Phase Extraction; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D058185:Magnetite Nanoparticles; D010937:Plant Growth Regulators; D011109:Polymethacrylic Acids; C034515:phenylalanine methyl ester; D015084:2,4-Dichlorophenoxyacetic Acid; D010649:Phenylalanine; C007017:4-chlorophenoxyacetic acid
keywords: High performance liquid chromatography; Magnetic solid-phase extraction; Plant growth regulator; Polymer synthesis