title: Inhibiting CREPT reduces the proliferation and migration of non-small cell lung cancer cells by down-regulating cell cycle related protein.
abstract: It has been reported that CREPT acts as a highly expressed oncogene in a variety of tumors, affecting cyclin D1 signal pathways. However, the distribution and clinical significance of CREPT in NSCLC remains poorly understood. Our study focused on the role of CREPT on the regulation ofnon-small cell lung cancer (NSCLC). We found that CREPT mRNA and protein expression was significantly increased in NSCLC compared with adjacent lung tissues and was increased in various NSCLC cell lines compared with the normal human bronchial epithelial (HBE) cell line. siRNA-induced knockingdown of CREPT significantly inhibited the proliferation and migration of NSCLC cell lines by arresting cell cycle in S phase. Moreover, CREPT knocking down affected the expression of cell cycle proteins including c-mycand CDC25A. Finally, we found there were obvious correlations between CREPT with c-myc expression in histological type, differentiation, and pTNM stages of NSCLC (P<0.05, rs>0.3). Immunohistofluorescence studies demonstrated a co-localization phenomenon when CREPT and c-myc were expressed. Thus, we propose that CREPT may promote NSCLC cell growth and migration through the c-myc and CDC25A signaling molecules.
authors.affiliation: Department of Thoracic Surgery, Tangdu Hospital, The Fourth Military Medical University Xi'an 710038, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.affiliation: Department of Thoracic Surgery, Tangdu Hospital, The Fourth Military Medical University Xi'an 710038, China.
authors.forename: Wei-Miao
authors.initials: WM
authors.lastname: Li
authors.affiliation: Department of Thoracic Surgery, Tangdu Hospital, The Fourth Military Medical University Xi'an 710038, China.
authors.forename: Wu-Ping
authors.initials: WP
authors.lastname: Wang
authors.affiliation: Department of Thoracic Surgery, Tangdu Hospital, The Fourth Military Medical University Xi'an 710038, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Thoracic Surgery, Tangdu Hospital, The Fourth Military Medical University Xi'an 710038, China.
authors.forename: Yun-Feng
authors.initials: YF
authors.lastname: Ni
authors.affiliation: Department of Thoracic Surgery, Tangdu Hospital, The Fourth Military Medical University Xi'an 710038, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Xing
authors.affiliation: Department of Thoracic Surgery, Tangdu Hospital, The Fourth Military Medical University Xi'an 710038, China.
authors.forename: Jing-Hua
authors.initials: JH
authors.lastname: Xia
authors.affiliation: Department of Thoracic Surgery, Tangdu Hospital, The Fourth Military Medical University Xi'an 710038, China.
authors.forename: Xue-Jiao
authors.initials: XJ
authors.lastname: Wang
authors.affiliation: Department of Thoracic Surgery, Tangdu Hospital, The Fourth Military Medical University Xi'an 710038, China.
authors.forename: Zhi-Pei
authors.initials: ZP
authors.lastname: Zhang
authors.affiliation: Department of Thoracic Surgery, Tangdu Hospital, The Fourth Military Medical University Xi'an 710038, China.
authors.forename: Xiao-Fei
authors.initials: XF
authors.lastname: Li
keywords: CDC25A; CREPT; c-myc; co-localization; migration; proliferation