title: Protective effects of ulinastatin and methylprednisolone against radiation-induced lung injury in mice.
abstract: The effectiveness of ulinastatin and methylprednisolone in treating pathological changes in mice with radiation-induced lung injury (RILI) was evaluated. Forty C57BL/6 female mice received whole-chest radiation (1.5 Gy/min for 12 min) and were randomly allocated into Group R (single radiation, n = 10), Group U (ulinastatin treatment, n = 10), Group M (methylprednisolone treatment, n = 10), or Group UM (ulinastatin and methylprednisolone treatment, n = 10). Another 10 untreated mice served as controls (Group C). Pathological changes in lung tissue, pulmonary interstitial area density (PIAD) and expression levels of transforming growth factor β1 (TGF-β1) and tumor necrosis factor α (TNF-α) in lung tissue, serum and bronchoalveolar lavage fluid were determined. Alleviation of pathological changes in lung tissue was observed in Groups U, M and UM. Treatment with ulinastatin, methylprednisolone or both effectively delayed the development of fibrosis at 12 weeks after radiation. Ulinastatin, methylprednisolone or both could alleviate the radiation-induced increase in the PIAD (P < 0.05 or P < 0.01). Treatment with ulinastatin, methylprednisolone or both significantly reduced the expression of TNF-α, but not TGF-β1, at 9 weeks after radiation compared with Group R (P < 0.01). Ulinastatin and /: or methylprednisolone effectively decreased the level of TNF-α in lung tissue after RILI and inhibited both the inflammatory response and the development of fibrosis.
authors.affiliation: Intensive Care Unit, Cancer Hospital of Jilin Province, Changchun 130021, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Nephrology, Bethune First Hospital of Jilin University, Changchun 130021, China.
authors.forename: Yu-Jun
authors.initials: YJ
authors.lastname: Du
authors.affiliation: Intensive Care Unit, Cancer Hospital of Jilin Province, Changchun 130021, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Intensive Care Unit, Cancer Hospital of Jilin Province, Changchun 130021, China.
authors.forename: Guo-Xing
authors.initials: GX
authors.lastname: Zhang
authors.affiliation: Intensive Care Unit, Cancer Hospital of Jilin Province, Changchun 130021, China.
authors.forename: Ni
authors.initials: N
authors.lastname: Sun
authors.affiliation: Intensive Care Unit, Cancer Hospital of Jilin Province, Changchun 130021, China dyjlxr@126.com.
authors.forename: Xiu-Jiang
authors.initials: XJ
authors.lastname: Li
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid; D005260:Female; D006023:Glycoproteins / Q000494:pharmacology / Q000627:therapeutic use*; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D055370:Lung Injury / Q000097:blood / Q000188:drug therapy* / Q000209:etiology*; D008775:Methylprednisolone / Q000494:pharmacology / Q000627:therapeutic use*; D008810:Mice, Inbred C57BL; D011832:Radiation Injuries / Q000097:blood / Q000188:drug therapy*; D011837:Radiation-Protective Agents / Q000627:therapeutic use*; D053773:Transforming Growth Factor beta1 / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D006023:Glycoproteins; D011837:Radiation-Protective Agents; D053773:Transforming Growth Factor beta1; D014409:Tumor Necrosis Factor-alpha; C028665:urinastatin; D008775:Methylprednisolone
keywords: methylprednisolone; radiation-induced lung injury; transforming growth factor β1 (TGF-β1); tumor necrosis factor α (TNF-α); ulinastatin