title: Oligomycins and pamamycin homologs impair motility and induce lysis of zoospores of the grapevine downy mildew pathogen, Plasmopara viticola.
abstract: Four antibiotics (pamamycin, oligomycin A, oligomycin B and echinosporin) were isolated and characterized from the fermentation broth of the marine Streptomyces strains B8496 and B8739. Bioassays revealed that each of these compounds impaired motility and caused subsequent lysis of P. viticola zoospores in a dose- and time-dependent manner. Pamamycin displayed the strongest motility inhibitory and lytic activities (IC50 0.1 μg mL(-1)) followed by oligomycin B (IC50 0.15 and 0.2 μg mL(-1)) and oligomycin F (IC50 0.3 and 0.5 μg mL(-1)). Oligomycin A and echinosporin also showed motility inhibitory activities against the zoospores with IC50 values of 3.0 and 10.0 μg mL(-1), respectively. This is the first report of motility inhibitory and lytic activities of these antibiotics against zoospores of a phytopathogenic peronosporomycete. Structures of all the isolated compounds were determined based on detailed spectroscopic analysis.
authors.affiliation: Institute of Organic and Biomolecular Chemistry, Georg-August University Goettingen, Tammanstrasse 2, D-37077 Göttingen, Germany.
authors.forename: Zerihun T
authors.initials: ZT
authors.lastname: Dame
authors.affiliation: Department of Crop Science, Division of Plant Pathology and Plant Protection, Georg-August University Goettingen, Grisebachstrasse 6, D-37077 Göttingen, Germany Department of Biotechnology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Salna, Gazipur 1706, Bangladesh tofazzalislam@yahoo.com.
authors.forename: M Tofazzal
authors.initials: MT
authors.lastname: Islam
authors.affiliation: Alfred Wegner Institute for Polar and Marine Research, Am Handelshafen 12, D-27570 Bremerhaven, Germany.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Helmke
authors.affiliation: Department of Crop Science, Division of Plant Pathology and Plant Protection, Georg-August University Goettingen, Grisebachstrasse 6, D-37077 Göttingen, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: von Tiedemann
authors.affiliation: Institute of Organic and Biomolecular Chemistry, Georg-August University Goettingen, Tammanstrasse 2, D-37077 Göttingen, Germany.
authors.forename: Hartmut
authors.initials: H
authors.lastname: Laatsch
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D018942:Macrolides / Q000819:agonists / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D009840:Oligomycins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D009868:Oomycetes / Q000187:drug effects* / Q000472:pathogenicity* / Q000502:physiology; D010935:Plant Diseases / Q000382:microbiology; D018515:Plant Leaves / Q000382:microbiology; D013170:Spores / Q000187:drug effects*; D013302:Streptomyces / Q000737:chemistry / Q000378:metabolism; D027843:Vitis / Q000382:microbiology*
chemical_list: D000900:Anti-Bacterial Agents; D018942:Macrolides; D009840:Oligomycins; C031004:oligomycin A; C048335:oligomycin B; C024500:pamamycin
keywords: Plasmopara viticola, zoosporicide; antibiotics; marine Streptomyces spp