title: Host CD40 Is Essential for DCG Treatment Against Metastatic Lung Cancer.
abstract: OBJECTIVE
  For the application of invariant natural killer T (iNKT) cells in cancer therapy, the CD40-CD40L interaction is indispensable in administering alpha-galactosylceramide (αGalCer). We hypothesized that CD40 plays an important role in dendritic cells (DC) pulsed with αGalCer (DCGs) in the treatment of lung metastases.
  
  
  METHODS
  Wild-type (WT) and CD40(-/-) mice were treated with DCGs isolated from WT or CD40(-/-) mice in a B16F10 lung metastases model and NK and NKT cell activity in lungs and the spleen were examined.
  
  
  RESULTS
  DCG treatment improved WT mice survival but CD40(-/-) hosts received no survival benefit. Conversely, attenuation of a therapeutic effect in mice treated with CD40(-/-) DCGs was not observed. The functional activities of NK and NKT cells in DCG-treated CD40(-/-) mice were partially suppressed.
  
  
  CONCLUSIONS
  Host CD40 is essential for DCG treatment to have a therapeutic effect on B16F10 lung metastases.
authors.affiliation: Department of Surgery, Division of Gastrointestinal Surgery, Kobe University Graduate School of Medicine, Kobe, Japan kiyama@med.kobe-u.ac.jp.
authors.forename: Kimihiro
authors.initials: K
authors.lastname: Yamashita
authors.affiliation: Department of Surgery, Division of Gastrointestinal Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hasegawa
authors.affiliation: Departments of Microbiology and Acute Medicine, Kindai University Graduate School of Medical Sciences, Osaka, Japan.
authors.forename: Mitsugu
authors.initials: M
authors.lastname: Fujita
authors.affiliation: Department of Surgery, Division of Gastrointestinal Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
authors.forename: Masayasu
authors.initials: M
authors.lastname: Nishi
authors.affiliation: Department of Surgery, Division of Gastrointestinal Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Tanaka
authors.affiliation: Department of Surgery, Division of Gastrointestinal Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Arimoto
authors.affiliation: Department of Surgery, Division of Gastrointestinal Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Suzuki
authors.affiliation: Medinet Medical Institute, MEDINET Co., Ltd., Tokyo, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kamigaki
authors.affiliation: Department of Surgery, Division of Gastrointestinal Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kakeji
mesh_terms: D000818:Animals; D019013:CD40 Antigens / Q000378:metabolism*; D003713:Dendritic Cells / Q000187:drug effects / Q000637:transplantation*; D005260:Female; D005699:Galactosylceramides / Q000494:pharmacology; D007167:Immunotherapy; D007371:Interferon-gamma / Q000097:blood; D018664:Interleukin-12 / Q000097:blood; D007694:Killer Cells, Natural / Q000276:immunology; D008175:Lung Neoplasms / Q000556:secondary / Q000628:therapy*; D008546:Melanoma, Experimental / Q000556:secondary / Q000628:therapy*; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D055611:Natural Killer T-Cells / Q000276:immunology; D009368:Neoplasm Transplantation
chemical_list: D019013:CD40 Antigens; D005699:Galactosylceramides; C493154:alpha-galactosylceramide; D018664:Interleukin-12; D007371:Interferon-gamma
keywords: B16F10; CD40; DCG; NKT cell; lung metastasis; tumor immunity