title: Protein-directed synthesis of highly monodispersed, spherical gold nanoparticles and their applications in multidimensional sensing.
abstract: An in-situ reduction method has been reported to prepare gold nanoparticles (GNPs) of 40-110 nm by using the green reducing agents of proteins, which are activated by H2O2 and the superoxide anion (). The protein of collagen turns HAuCl4 to the aqueous Au(I) ainions, which are further reduced by other proteins to be highly monodispersed and spherical GNPs of different sizes. The GNPs reduced by different proteins are found to be with the exposed {100} facets, the distinctive UV-vis absorption spectra and various colors (See Fig. 1). By means of extracting the color responses, such as red, green and blue (RGB) alterations, an in-situ reduction method-based multidimensional sensing platform is fabricated in the process of GNPs synthesis. Without further modification of GNPs, nine common proteins are found to be well detected and discriminated at different concentrations. Moreover, this sensing platform also demonstrates great potentials in qualitative and semiquantitative analysis on the individuals of these proteins with high sensitivity. Furthermore, the validation of this multidimensional sensing platform has been carried out by analysis on the spiked proteins in human urine and the target proteins in complex matrix (e.g. lysozyme in human tear).
authors.affiliation: College of Physics and Electronic Engineering, Nanyang Normal University, Nanyang 473061, China.
authors.forename: Yumin
authors.initials: Y
authors.lastname: Leng
authors.affiliation: College of Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Fu
authors.affiliation: College of Chemistry and Pharmaceutical Engineering, Nanyang Normal University, Nanyang 473061, China.
authors.forename: Liqun
authors.initials: L
authors.lastname: Ye
authors.affiliation: College of Chemistry and Pharmaceutical Engineering, Nanyang Normal University, Nanyang 473061, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.affiliation: College of Physics and Electronic Engineering, Nanyang Normal University, Nanyang 473061, China.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Xu
authors.affiliation: College of Chemistry and Pharmaceutical Engineering, Nanyang Normal University, Nanyang 473061, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Xing
authors.affiliation: College of Physics and Electronic Engineering, Nanyang Normal University, Nanyang 473061, China.
authors.forename: Junbao
authors.initials: J
authors.lastname: He
authors.affiliation: College of Physics and Electronic Engineering, Nanyang Normal University, Nanyang 473061, China.
authors.forename: Yuling
authors.initials: Y
authors.lastname: Song
authors.affiliation: College of Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China.
authors.forename: Chaoliang
authors.initials: C
authors.lastname: Leng
authors.affiliation: College of Chemistry and Pharmaceutical Engineering, Nanyang Normal University, Nanyang 473061, China.
authors.forename: Yongming
authors.initials: Y
authors.lastname: Guo
authors.affiliation: College of Physics and Electronic Engineering, Nanyang Normal University, Nanyang 473061, China.
authors.forename: Xiaoxu
authors.initials: X
authors.lastname: Ji
authors.affiliation: College of Physics and Electronic Engineering, Nanyang Normal University, Nanyang 473061, China.
authors.forename: Zhiwen
authors.initials: Z
authors.lastname: Lu
mesh_terms: D004396:Coloring Agents / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D010084:Oxidation-Reduction; D011506:Proteins / Q000032:analysis / Q000737:chemistry; D012996:Solutions; D013666:Tears / Q000201:enzymology; D016482:Urinalysis
chemical_list: D004396:Coloring Agents; D011506:Proteins; D012996:Solutions; D006046:Gold; D006861:Hydrogen Peroxide