title: Overexpression of C/EBPβ Affects The Cell Cycle Regulators and Spermatogenesis Related Genes Expression And Function of Bovine Sertoli Cells.
abstract: CCAAT/enhancer-binding protein beta (C/EBPβ), an important transcriptional factor, plays a pivotal role in the regulation of female germ cell development. However, the role of C/EBPβ on the development of male germ cells has not been reported. In this study, we constructed the recombinant adenovirus plasmids of bovine C/EBPβ gene and harvested the subsequent adenoviruses, and then assessed the mRNA levels of spermatogenesis-related genes (real-time PCR) and secretion of inhibin B after 48 h of Ad-C/EBPβ recombinant adenovirus infection in bovine sertoli cells (SCs). We found that overexpression of exogenous C/EBPβ gene upregulated the mRNA expressions of spermatogenesis-related genes, including Pdgfa, Claudin, Caspase-3, Occludin, kit1 and Cyclin E, and decreased the mRNA levels of Cyclin D1. Meanwhile, overexpression of exogenous C/EBPβ gene significantly increases the amounts of secreted inhibin B. In conclusion, the results indicate that the C/EBPβ gene plays an important regulatory role in regulation of the cell cycle regulators and spermatogenesis-related genes expression and function of bovine SCs.
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Shaanxi, China.
authors.forename: K
authors.initials: K
authors.lastname: Tang
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Shaanxi, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Jin
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Shaanxi, China.
authors.forename: F
authors.initials: F
authors.lastname: Chen
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Shaanxi, China.
authors.forename: L
authors.initials: L
authors.lastname: Wang
mesh_terms: D000256:Adenoviridae; D000818:Animals; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000235:genetics / Q000378:metabolism*; D002417:Cattle; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology; D018014:Gene Transfer Techniques; D007265:Inhibins / Q000235:genetics / Q000378:metabolism; D008297:Male; D012708:Sertoli Cells / Q000378:metabolism*; D013091:Spermatogenesis / Q000502:physiology*
chemical_list: D022782:CCAAT-Enhancer-Binding Protein-beta; D018797:Cell Cycle Proteins; C422364:inhibin B; D007265:Inhibins