title: [Expression and clinical significance of autophagy-related gene Beclin1 and P62 in nasal polyps].
abstract: OBJECTIVE
  To investigate the expression of autophagy-related gene Beclin1 and P62 in nasal polyps and its relationship with the pathogenesis of this disease.
  
  
  METHODS
  The specimens were divided into two groups: nasal polyp tissue(n=50) and normal inferior turbinate mucosa(n=20). The general morphology was detected with hematoxylin-eosin(HE) staining, the expression of Beclin1 and P62 was examined with immunohistochemistry(IHC) and real-time fluorescent quantitative reverse transcription-polymerase chain reaction ( RT-PCR). SPSS 20.0 software was used to analyze the data.
  
  
  RESULTS
  Protein level: The expression of Beclin1 in nasal polyp tissue was lower than inferior turbinate mucosa(U=-13.36, P<0.01), in contrast, P62 in experimental group was higher than control group(U=12.99 , P<0.01). mRNA level: The relative quantity of Beclin1 and LC3B expressions in nasal polyp were 0.46±0.17 and 0.46±0.11, which was lower than those in turbinate mucosa 1.11±0.47 and 0.96±0.25.The differences were significant(t value was -4.61, -4.61, both P<0.01). But the relative quantity of P62 expression in nasal polyp was 2.19±0.44, which was higher than that in turbinate mucosa (1.05±0.33). The difference was all significant(t=6.16, P<0.01).
  
  
  CONCLUSIONS
  Compared with control group, the expression of Beclin1 was deficient and P62 was much more. Autophagy was deficient in nasal polyps, which might be in connection with the pathogenesis of the disease.
authors.affiliation: Department of Otorhinolaryngology, Qilu Hospital of Shandong University, Key Laboratory of Otorhino laryngology, Chinese Ministry of Health, Jinan 250012, China.
authors.forename: J J
authors.initials: JJ
authors.lastname: Qi
authors.affiliation: Department of Otorhinolaryngology, Central Hospital of Zibo, Zibo 255000, China.
authors.forename: X F
authors.initials: XF
authors.lastname: Han
authors.affiliation: Department of Otorhinolaryngology, Qilu Hospital of Shandong University, Key Laboratory of Otorhino laryngology, Chinese Ministry of Health, Jinan 250012, China.
authors.forename: X L
authors.initials: XL
authors.lastname: Cai
authors.affiliation: Department of Otorhinolaryngology, Qilu Hospital of Shandong University, Key Laboratory of Otorhino laryngology, Chinese Ministry of Health, Jinan 250012, China.
authors.forename: X Z
authors.initials: XZ
authors.lastname: Li
authors.affiliation: Department of Otorhinolaryngology, Qilu Hospital of Shandong University, Key Laboratory of Otorhino laryngology, Chinese Ministry of Health, Jinan 250012, China.
authors.forename: L Q
authors.initials: LQ
authors.lastname: Zhang
authors.affiliation: Department of Otorhinolaryngology, Qilu Hospital of Shandong University, Key Laboratory of Otorhino laryngology, Chinese Ministry of Health, Jinan 250012, China.
authors.forename: X
authors.initials: X
authors.lastname: Feng
authors.affiliation: Department of Otorhinolaryngology, Qilu Hospital of Shandong University, Key Laboratory of Otorhino laryngology, Chinese Ministry of Health, Jinan 250012, China.
authors.forename: D Y
authors.initials: DY
authors.lastname: Liu
mesh_terms: D001343:Autophagy / Q000235:genetics*; D000071186:Beclin-1 / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009297:Nasal Mucosa / Q000378:metabolism; D009298:Nasal Polyps / Q000235:genetics* / Q000473:pathology; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D014420:Turbinates / Q000378:metabolism
chemical_list: D000071186:Beclin-1; D009363:Neoplasm Proteins; C118585:P62 protein, human; D012333:RNA, Messenger; D016601:RNA-Binding Proteins