title: Oxalic acid degradation by a novel fungal oxalate oxidase from Abortiporus biennis.
abstract: Oxalate oxidase was identified in mycelial extracts of a basidiomycete Abortiporus biennis strain. Intracellular enzyme activity was detected only after prior lowering of the pH value of the fungal cultures by using oxalic or hydrochloric acids. This enzyme was purified using size exclusion chromatography (Sephadex G-25) and ion-exchange chromatography (DEAE-Sepharose). This enzyme exhibited optimum activity at pH 2 when incubated at 40°C, and the optimum temperature was established at 60°C. Among the tested organic acids, this enzyme exhibited specificity only towards oxalic acid. Molecular mass was calculated as 58 kDa. The values of Km for oxalate and Vmax for the enzyme reaction were 0.015 M and 30 mmol min(-1), respectively.
authors.affiliation: Department of Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Grąz
authors.affiliation: Department of Genetics and Microbiology, Maria Curie-Skłodowska University, Lublin, Poland.
authors.forename: Kamila
authors.initials: K
authors.lastname: Rachwał
authors.affiliation: Department of Genetics and Microbiology, Maria Curie-Skłodowska University, Lublin, Poland.
authors.forename: Radosław
authors.initials: R
authors.lastname: Zan
authors.affiliation: Department of Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Jarosz-Wilkołazka
mesh_terms: D001487:Basidiomycota / Q000201:enzymology*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D025282:Mycelium / Q000201:enzymology*; D019815:Oxalic Acid / Q000737:chemistry*; D010088:Oxidoreductases / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D005656:Fungal Proteins; D019815:Oxalic Acid; D010088:Oxidoreductases; C020598:oxalate oxidase