title: Marinococcus salis sp., nov., a moderately halophilic bacterium isolated from a salt marsh.
abstract: A novel Gram-stain-positive, coccoid-shaped, facultative anaerobic, motile and halophilic bacterium strain 5MTwas isolated from Surajbari in India. Based on the 16S rRNA gene sequence analysis, it was identified as belonging to the genus Marinococcus and was most closely related to Marinococcus luteus KCTC 13214T(99.3 %, sequence similarity), Marinococcus halotolerans KCTC 19045T(99.0 %), Marinococcus halophilus LMG 17439T(98.8 %) and Marinococcus tarijensis LMG 26930T(98.7 %). However, the DNA-DNA relatedness of strain 5MTwith M. luteus KCTC 13214T, M. halotolerans KCTC 19045T, M. halophilus LMG 17439Tand M. tarijensis LMG 26930Twas 42.6 ± 0.8, 48.6 ± 0.8, 40.9 ± 0.8 and 39.8 ± 0.9 %, respectively. Strain 5MTgrows optimally at 5 % (w/v) NaCl, pH 7.5-8.5 and 37 °C. The cell-wall peptidoglycan of strain 5MTcontains meso-diaminopimelic acid. Polar lipids of the strain 5MTinclude diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, a phospholipid and two unknown lipids. The predominant isoprenoid quinone was MK-7. DNA G+C content was 48.9 mol%, and anteiso-C15:0(40.9 %) was the predominant fatty acid. The results of phylogenetic, biochemical tests and chemotaxonomic allowed a clear differentiation of strain 5MTfrom all of its nearest phylogenetic neighbours, which represents a novel member of the genus Marinococcus, for which the name Marinococcus salis sp., nov., is proposed. The type strain is 5MT(=KCTC 33743T= LMG 29101T= CGMCC 1.15385T).
authors.affiliation: Microbial Ecology Laboratory, Department of Biochemistry, Mahatma Gandhi University, Anneparthy, Yellareddygudem (PO), Nalgonda, Telangana, 508254, India. svr.micro@gmail.com.
authors.forename: Sultanpuram
authors.initials: S
authors.lastname: Vishnuvardhan Reddy
authors.affiliation: Microbial Ecology Laboratory, Department of Biochemistry, Mahatma Gandhi University, Anneparthy, Yellareddygudem (PO), Nalgonda, Telangana, 508254, India.
authors.forename: Mothe
authors.initials: M
authors.lastname: Thirumala
authors.affiliation: Microbial Ecology Laboratory, Department of Biochemistry, Mahatma Gandhi University, Anneparthy, Yellareddygudem (PO), Nalgonda, Telangana, 508254, India.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Farooq
authors.affiliation: Bacterial Discovery Laboratory, Centre for Environment, Institute of Science and Technology, J. N. T. University, Kukatpally, Hyderabad, Telangana, 500085, India.
authors.forename: Chintalapati
authors.initials: C
authors.lastname: Sasikala
authors.affiliation: Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad, Telangana, 500046, India.
authors.forename: Chintalapati
authors.initials: C
authors.lastname: Venkata Ramana
mesh_terms: D001406:Bacillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D007194:India; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000032:analysis; D053833:Wetlands
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D003960:Diaminopimelic Acid
keywords: Marinococcus salis sp. nov.; Moderate halophile; Polyphasic taxonomy