title: Endophytic Streptomyces sp. AC35, a producer of bioactive isoflavone aglycones and antimycins.
abstract: In this research, a microbial endophytic strain obtained from the rhizosphere of the conifer Taxus baccata and designated as Streptomyces sp. AC35 (FJ001754.1 Streptomyces, GenBank) was investigated. High 16S rDNA gene sequence similarity suggests that this strain is closely related to S. odorifer. The major fatty acid profile of intracellular lipids was also carried out to further identify this strain. Atomic force microscopy and scanning acoustic microscopy were used to image our strain. Its major excreted substances were extracted, evaluated for antimicrobial activity, purified, and identified by ultraviolet-visible spectroscopy (UV-vis), liquid chromatography-mass spectrometry (LC-MS/MS) and nuclear magnetic resonance as the bioactive isoflavone aglycones-daidzein, glycitein and genistein. Batch cultivation, performed under different pH conditions, revealed enhanced production of antimycin components when the pH was stable at 7.0. Antimycins were detected by HPLC and identified by UV-vis and LC-MS/MS combined with the multiple reaction monitoring. Our results demonstrate that Streptomyces sp. AC35 might be used as a potential source of effective, pharmaceutically active compounds.
authors.affiliation: Institute of Biotechnology, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Bratislava, Slovakia. petracibik@yahoo.com.
authors.forename: P
authors.initials: P
authors.lastname: Ondrejíčková
authors.affiliation: Institute of Biotechnology, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Bratislava, Slovakia.
authors.forename: M
authors.initials: M
authors.lastname: Šturdíková
authors.affiliation: Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, Bratislava, Slovakia.
authors.forename: A
authors.initials: A
authors.lastname: Hushegyi
authors.affiliation: Department of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.
authors.forename: E
authors.initials: E
authors.lastname: Švajdlenka
authors.affiliation: Institute of Biotechnology, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Bratislava, Slovakia.
authors.forename: K
authors.initials: K
authors.lastname: Markošová
authors.affiliation: Institute of Biotechnology, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Bratislava, Slovakia.
authors.forename: M
authors.initials: M
authors.lastname: Čertík
mesh_terms: D000968:Antimycin A / Q000031:analogs & derivatives / Q000378:metabolism*; D019833:Genistein / Q000378:metabolism; D007529:Isoflavones / Q000096:biosynthesis*; D013302:Streptomyces / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D007529:Isoflavones; C004742:daidzein; D000968:Antimycin A; C086566:glycitein; D019833:Genistein
keywords: Antimycin; Atomic force microscopy; Isoflavone aglycones; Streptomyces sp.