title: A New Thiosemicarbazone-Based Fluorescence "Turn-on" Sensor for Zn(2+) Recognition with a Large Stokes Shift and its Application in Live Cell Imaging.
abstract: Selective fluorescence turn on Zn(2+) sensor with long-wavelength emission and a large Stokes shift is highly desirable in Zn(2+) sensing area. We reported herein the synthesis and Zn(2+) recognition properties of a new thiosemicarbazone-based fluorescent sensor L. L displays high selectivity and sensitivity toward Zn(2+) over other metal ions in DMSO-H2O (1:1, v/v, HEPES 10 mM, pH = 7.4) solution with a long-wavelength emission at 572 nm and a large Stokes shift of 222 nm. Confocal fluorescence microscopy experiments demonstrate that L is cell-permeable and capable of monitoring intracellular Zn(2+). Graphical Abstract We report a new thiosemicarbazone-based fluorescent sensor (L) for selective recognition of Zn(2+) with a long wavelength emission and a large Stokes shift.
authors.affiliation: Department of Chemistry, Bohai University, Jinzhou, 121013, China. ljtang@bhu.edu.cn.
authors.forename: Lijun
authors.initials: L
authors.lastname: Tang
authors.affiliation: Department of Chemistry, Bohai University, Jinzhou, 121013, China.|State Key Laboratory of Fine Chemicals, Dalian University of Technology, No. 2 Linggong Road, High-tech District, Dalian, 116024, China.
authors.forename: Zhenlong
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Department of Chemistry, Bohai University, Jinzhou, 121013, China.
authors.forename: Zhuxuan
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: Department of Chemistry, Bohai University, Jinzhou, 121013, China.
authors.forename: Keli
authors.initials: K
authors.lastname: Zhong
authors.affiliation: Department of Chemistry, Bohai University, Jinzhou, 121013, China.
authors.forename: Yanjiang
authors.initials: Y
authors.lastname: Bian
mesh_terms: D002470:Cell Survival; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D018613:Microscopy, Confocal; D009369:Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism* / Q000473:pathology; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013882:Thiosemicarbazones / Q000737:chemistry*; D014407:Tumor Cells, Cultured; D015032:Zinc / Q000032:analysis*
chemical_list: D005456:Fluorescent Dyes; D013882:Thiosemicarbazones; D015032:Zinc
keywords: Fluorescence; Thiosemicarbazone; Zn2+ sensor