title: Biochip for the Detection of Bacillus anthracis Lethal Factor and Therapeutic Agents against Anthrax Toxins.
abstract: Tethered lipid bilayer membranes (tBLMs) have been used in many applications, including biosensing and membrane protein structure studies. This report describes a biosensor for anthrax toxins that was fabricated through the self-assembly of a tBLM with B. anthracis protective antigen ion channels that are both the recognition element and electrochemical transducer. We characterize the sensor and its properties with electrochemical impedance spectroscopy and surface plasmon resonance. The sensor shows a sensitivity similar to ELISA and can also be used to rapidly screen for molecules that bind to the toxins and potentially inhibit their lethal effects.
authors.affiliation: Physical Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899-8120, USA. vitalii.silin@nist.gov.|NIST Center for Neutron Research, National Institute of Standards and Technology, Gaithersburg, MD 20899-8120, USA. vitalii.silin@nist.gov.|Institute for Bioscience and Biotechnology Research, University of Maryland, Rockville, MD 20899, USA. vitalii.silin@nist.gov.
authors.forename: Vitalii
authors.initials: V
authors.lastname: Silin
authors.affiliation: Physical Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899-8120, USA. john.kasianowicz@nist.gov.
authors.forename: John J
authors.initials: JJ
authors.lastname: Kasianowicz
authors.affiliation: Physical Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899-8120, USA. lasirenita54@yahoo.com.
authors.forename: Ariel
authors.initials: A
authors.lastname: Michelman-Ribeiro
authors.affiliation: US Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702-5011, USA. rekha.g.panchal.civ@mail.mil.
authors.forename: Rekha G
authors.initials: RG
authors.lastname: Panchal
authors.affiliation: US Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702-5011, USA. sina.bavari.civ@mail.mil.
authors.forename: Sina
authors.initials: S
authors.lastname: Bavari
authors.affiliation: Physical Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899-8120, USA. joseph.robertson@nist.gov.
authors.forename: Joseph W F
authors.initials: JW
authors.lastname: Robertson
keywords: anthrax; biochip; edema factor; lethal factor; protective antigen; screening; tethered bilayer membrane; therapeutic agents