title: Comparison of Xpert®HPV and Hybrid Capture®2 DNA Test™ for detection of high-risk HPV infection in cervical atypical squamous cells of undetermined significance.
abstract: This study compares Xpert®HPV and Hybrid Capture®2 High-Risk HPV DNA Test™ (hc2) for the detection of high-risk HPV infection in cervical smears. Papanicolaou smears with atypical squamous cells of undetermined significance (ASC-US) constituted the study specimens. Of the 168 ASC-US samples, 134 (79.8%) were from Saudi patients. The hc2 test was positive in 33 (19.6%) of the total patients, 20% among Saudi patients, and 17.6% among non-Saudi patients. Xpert®HPV produced positive results in 30 (17.8%) of the samples. The overall concordance rate between the two tests was 98.2%, and the positive concordance rate was 91%. There were three samples tested positive by hc2 that tested negative by Xpert®HPV. HPV 16, HPV 18/45 and HPV other types were the most common types. Both tests have a reasonable positive concordance rate, although hc2 detected more cases than Xpert®HPV. Xpert®HPV provides a viable alternative to the hc2 test with similar detection results for samples with ASC-US.
authors.affiliation: Molecular Diagnostic Laboratory, Johns Hopkins Aramco Healthcare, Dhahran, Saudi Arabia.
authors.forename: Ali A
authors.initials: AA
authors.lastname: Rabaan
authors.affiliation: General Laboratory Services Unit, Johns Hopkins Aramco Healthcare, Dhahran, Saudi Arabia.
authors.forename: Donald R
authors.initials: DR
authors.lastname: Taylor
authors.affiliation: Pathology Services Division, Johns Hopkins Aramco Healthcare, Dhahran, Saudi Arabia.
authors.forename: Mohammed F
authors.initials: MF
authors.lastname: Dawamneh
authors.affiliation: Specialty Internal Medicine, Johns Hopkins Aramco Healthcare, Dhahran, Saudi Arabia; Indiana University School of Medicine, Indianapolis, USA. Electronic address: jaffar.tawfiq@jhah.com.
authors.forename: Jaffar A
authors.initials: JA
authors.lastname: Al-Tawfiq
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D065309:Atypical Squamous Cells of the Cervix / Q000821:virology*; D005260:Female; D006801:Humans; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000821:virology; D014626:Vaginal Smears; D055815:Young Adult
keywords: ASC-US; Atypical squamous cells of undetermined significance; HPV; Human Papilloma virus