title: Filling a GAP-An Optimized Probe for ER Ca(2+) Imaging In Vivo.
abstract: In this issue of Cell Chemical Biology, Navas-Navarro et al. (2016) demonstrate that fusion of engineered derivatives of the long-known jellyfish proteins green fluorescent protein (GFP) and aequorin yield optimized genetically encoded fluorescent probes for detecting Ca(2+) signals within the endoplasmic reticulum (ER) of living animals.
authors.affiliation: Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University of Graz, Harrachgasse 21/III, 8010 Graz, Austria. Electronic address: roland.malli@medunigraz.at.
authors.forename: Roland
authors.initials: R
authors.lastname: Malli
authors.affiliation: Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University of Graz, Harrachgasse 21/III, 8010 Graz, Austria.
authors.forename: Emrah
authors.initials: E
authors.lastname: Eroglu
authors.affiliation: Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University of Graz, Harrachgasse 21/III, 8010 Graz, Austria.
authors.forename: Markus
authors.initials: M
authors.lastname: Waldeck-Weiermair
authors.affiliation: Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University of Graz, Harrachgasse 21/III, 8010 Graz, Austria.
authors.forename: Wolfgang F
authors.initials: WF
authors.lastname: Graier
mesh_terms: D000331:Aequorin; D000818:Animals; D002118:Calcium; D004721:Endoplasmic Reticulum; D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins
chemical_list: D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins; D000331:Aequorin; D002118:Calcium