title: Arabidopsis CSLD5 Functions in Cell Plate Formation in a Cell Cycle-Dependent Manner.
abstract: In plants, the presence of a load-bearing cell wall presents unique challenges during cell division. Unlike other eukaryotes, which undergo contractile cytokinesis upon completion of mitosis, plants instead synthesize and assemble a new dividing cell wall to separate newly formed daughter cells. Here, we mine transcriptome data from individual cell types in the Arabidopsis thaliana stomatal lineage and identify CSLD5, a member of the Cellulose Synthase Like-D family, as a cell wall biosynthesis enzyme uniquely enriched in rapidly dividing cell populations. We further show that CSLD5 is a direct target of SPEECHLESS, the master transcriptional regulator of these divisions during stomatal development. Using a combination of genetic analysis and in vivo localization of fluorescently tagged fusion proteins, we show that CSLD5 preferentially accumulates in dividing plant cells where it participates in the construction of newly forming cell plates. We show that CSLD5 is an unstable protein that is rapidly degraded upon completion of cell division and that the protein turnover characteristics of CSLD5 are altered in ccs52a2 mutants, indicating that CSLD5 turnover may be regulated by a cell cycle-associated E3-ubiquitin ligase, the anaphase-promoting complex.
authors.affiliation: Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109-1048.
authors.forename: Fangwei
authors.identifier: 0000-0002-3422-6919
authors.initials: F
authors.lastname: Gu
authors.affiliation: Department of Biology, Stanford University, Stanford, California 94305-5020.
authors.forename: Martin
authors.identifier: 0000-0003-4289-7605
authors.initials: M
authors.lastname: Bringmann
authors.affiliation: Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109-1048.
authors.forename: Jonathon R
authors.identifier: 0000-0001-8277-279X
authors.initials: JR
authors.lastname: Combs
authors.affiliation: Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109-1048.
authors.forename: Jiyuan
authors.identifier: 0000-0003-0185-1133
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Biology, Stanford University, Stanford, California 94305-5020 HHMI, Stanford University, Stanford, California 94305-5020.
authors.forename: Dominique C
authors.identifier: 0000-0003-0873-3543
authors.initials: DC
authors.lastname: Bergmann
authors.affiliation: Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109-1048 nielsene@umich.edu.
authors.forename: Erik
authors.identifier: 0000-0003-3565-0073
authors.initials: E
authors.lastname: Nielsen
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D002453:Cell Cycle / Q000235:genetics / Q000502:physiology; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D005964:Glucosyltransferases / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D051792:Basic Helix-Loop-Helix Proteins; D005964:Glucosyltransferases; D044767:Ubiquitin-Protein Ligases; C517480:SPEECHLESS protein, Arabidopsis; C526694:CSLD5 protein, Arabidopsis