title: Simultaneous Determination of Hydrochlorothiazide and Losartan Potassium in Osmotic Pump Tablets by Microemulsion Liquid Chromatography.
abstract: A rapid and efficient oil-in-water microemulsion liquid chromatographic (MELC) method has been optimized and validated for the determination of hydrochlorothiazide (HCT) and losartan potassium (LOP) in osmotic pump tablets. Samples were injected into a C18 (150 mm × 4.6 mm ID, 5 µm particle size) analytical column, which was maintained at 30°C. The most effective MELC system had a mobile phase consisting of 95% (v/v) of 3.0% (w/w) SDS, 6.0% (w/w) n-butanol, 0.8% (w/w) n-octane, 90.2% (w/w) water and 5% (v/v) acetonitrile (pH 5). The flow rate was 1.0 mL min(-1) and UV detection was performed at 265 nm. Linearity ranged from 2.5 to 12.5 µg mL(-1) for HCT and 10.0-60.0 µg mL(-1) for LOP (r > 0.999 for both drugs). The proposed method was rapid, precise (RSDs < 1.4%) and accurate (98.9% recovery for HCT and 101% recovery for LOP). It is applicable to simultaneous determination of HCT and LOP in osmotic pump tablets.
authors.affiliation: Department of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China.
authors.forename: Liangxing
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.
authors.forename: Caiyun
authors.initials: C
authors.lastname: Lai
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.
authors.forename: Xueyi
authors.initials: X
authors.lastname: Xuan
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.
authors.forename: Chongkai
authors.initials: C
authors.lastname: Gao
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China 13724117338@163.com.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000295:instrumentation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D004655:Emulsions / Q000737:chemistry; D006852:Hydrochlorothiazide / Q000032:analysis*; D019808:Losartan / Q000032:analysis*; D015203:Reproducibility of Results
chemical_list: D004655:Emulsions; D006852:Hydrochlorothiazide; D019808:Losartan