title: Derivation of the human embryonic stem cell line RCe014-A (RC-10).
abstract: The human embryonic stem cell line RCe014-A (RC-10) was derived from a fresh oocyte voluntarily donated as unsuitable and surplus to fertility requirements following ethics committee approved informed consent under licence from the UK Human Fertilisation and Embryology Authority. The cell line shows normal pluripotency marker expression and differentiation to the three germ layers in vitro. It has a mixed 46XY and 47XY +12 male karyotype and microsatellite PCR identity, HLA and blood group typing data is available.
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh, EH16 4UX, UK; Centre for Clinical Brain Sciences, University of Edinburgh, UK; MRC Centre for Regenerative Medicine, University of Edinburgh, UK. Electronic address: paul.desousa@ed.ac.uk.
authors.forename: P A
authors.initials: PA
authors.lastname: De Sousa
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh, EH16 4UX, UK.
authors.forename: B J
authors.initials: BJ
authors.lastname: Tye
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh, EH16 4UX, UK.
authors.forename: K
authors.initials: K
authors.lastname: Bruce
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh, EH16 4UX, UK.
authors.forename: P
authors.initials: P
authors.lastname: Dand
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh, EH16 4UX, UK.
authors.forename: G
authors.initials: G
authors.lastname: Russell
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh, EH16 4UX, UK.
authors.forename: D M
authors.initials: DM
authors.lastname: Collins
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh, EH16 4UX, UK.
authors.forename: A
authors.initials: A
authors.lastname: Greenshields
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh, EH16 4UX, UK.
authors.forename: H
authors.initials: H
authors.lastname: Bradburn
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh, EH16 4UX, UK.
authors.forename: J M
authors.initials: JM
authors.lastname: Downie
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh, EH16 4UX, UK.
authors.forename: M
authors.initials: M
authors.lastname: Bateman
authors.affiliation: Roslin Cells Limited, Nine Edinburgh Bio-Quarter, 9 Little France Road, Edinburgh, EH16 4UX, UK.
authors.forename: A
authors.initials: A
authors.lastname: Courtney
mesh_terms: D002454:Cell Differentiation; D002478:Cells, Cultured; D002881:Chromosomes, Human, Pair 12; D004622:Embryo, Mammalian / Q000166:cytology; D058732:Embryoid Bodies / Q000166:cytology; D005838:Genotype; D006650:Histocompatibility Testing; D000066449:Human Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D059785:Karyotype; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D008856:Microscopy, Fluorescence; D009865:Oocytes / Q000166:cytology; D014157:Transcription Factors / Q000378:metabolism; D014314:Trisomy
chemical_list: D014157:Transcription Factors