title: Brown adipose tissue transplantation ameliorates male fertility impairment caused by diet-induced obesity.
abstract: Populations with obesity or overweight have a high incidence of infertility. We hypothesised that brown adipose tissue (BAT) transplantation can attenuate the impairment of male fertility caused by diet-induced obesity. BATs were transplanted from male donor mice into age and sex matched recipient mice fed high-fat diets (HFD). Sperm motility experiment was conducted after surgical procedure. X-ray computed tomography scanning, biochemical assay, real-time PCR and western blot analysis were performed. BAT transplantation reduced body fat and epididymal fat mass, as well as triglycerides (TG) content in testis and epididymis and total cholesterol (TCHO) contents in epididymis compared with the HFD group. Sperm motility and progressiveness were recovered and mRNA and protein levels of genes related to sperm motility such as cullin 3 (Cul3), peroxisome proliferator activated receptor alpha (PPARα) and its down-stream genes were significantly down-regulated post BAT transplantation. BAT transplantation partially ameliorated impairment of male fertility caused by diet-induced obesity.
authors.affiliation: Department of Laboratory Medicine, Bengbu Medical College, Bengbu 233030, PR China. Electronic address: shooterlau1220@aliyun.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.affiliation: Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Xiaomeng
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Preventive Medicine, Bengbu Medical College, Bengbu 233030, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.affiliation: Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Nan
authors.initials: N
authors.lastname: Sheng
mesh_terms: D002001:Adipose Tissue, Brown / Q000378:metabolism / Q000637:transplantation*; D000818:Animals; D044842:Cullin Proteins / Q000378:metabolism; D004032:Diet / Q000009:adverse effects*; D015536:Down-Regulation; D004822:Epididymis / Q000378:metabolism; D007248:Infertility, Male / Q000209:etiology* / Q000378:metabolism / Q000601:surgery*; D008297:Male; D051379:Mice; D009765:Obesity / Q000150:complications* / Q000378:metabolism; D047493:PPAR alpha / Q000378:metabolism; D013737:Testis / Q000378:metabolism; D016378:Tissue Transplantation / Q000379:methods*; D014280:Triglycerides / Q000378:metabolism
chemical_list: C503646:Cul3 protein, mouse; D044842:Cullin Proteins; D047493:PPAR alpha; D014280:Triglycerides
keywords: Brown adipose tissue (BAT); High fat diet (HFD); Male fertility; Tissue transplantation