title: High resolution observed in 800 MHz DNP spectra of extremely rigid type III secretion needles.
abstract: The cryogenic temperatures at which dynamic nuclear polarization (DNP) solid-state NMR experiments need to be carried out cause line-broadening, an effect that is especially detrimental for crowded protein spectra. By increasing the magnetic field strength from 600 to 800 MHz, the resolution of DNP spectra of type III secretion needles (T3SS) could be improved by 22 %, indicating that inhomogeneous broadening is not the dominant effect that limits the resolution of T3SS needles under DNP conditions. The outstanding spectral resolution of this system under DNP conditions can be attributed to its low overall flexibility.
authors.affiliation: Department of Molecular Biophysics, Leibniz-Institut für Molekulare Pharmakologie, 13125, Berlin, Germany.
authors.forename: Pascal
authors.initials: P
authors.lastname: Fricke
authors.affiliation: NMR Research Group, Bijvoet Center for Biomolecular Research, Utrecht University, 3584 CH, Utrecht, The Netherlands.
authors.forename: Deni
authors.initials: D
authors.lastname: Mance
authors.affiliation: Department of Molecular Biophysics, Leibniz-Institut für Molekulare Pharmakologie, 13125, Berlin, Germany.
authors.forename: Veniamin
authors.initials: V
authors.lastname: Chevelkov
authors.affiliation: Department of NMR-Based Structural Biology, Max Planck Institute for Biophysical Chemistry, 37077, Göttingen, Germany.
authors.forename: Karin
authors.initials: K
authors.lastname: Giller
authors.affiliation: Department of NMR-Based Structural Biology, Max Planck Institute for Biophysical Chemistry, 37077, Göttingen, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Becker
authors.affiliation: NMR Research Group, Bijvoet Center for Biomolecular Research, Utrecht University, 3584 CH, Utrecht, The Netherlands.
authors.forename: Marc
authors.initials: M
authors.lastname: Baldus
authors.affiliation: Department of Molecular Biophysics, Leibniz-Institut für Molekulare Pharmakologie, 13125, Berlin, Germany. alange@fmp-berlin.de.|Institut für Biologie, Humboldt-Universität zu Berlin, 10115, Berlin, Germany. alange@fmp-berlin.de.
authors.forename: Adam
authors.initials: A
authors.lastname: Lange
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D000072417:Protein Domains; D013696:Temperature; D000069296:Type III Secretion Systems / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D000069296:Type III Secretion Systems
keywords: DNP; Dynamics; PRE; Proteins; Solid-State NMR; Type III Secretion System