title: Lateral flow strip for visual detection of K-ras mutations based on allele-specific PCR.
abstract: OBJECTIVE
  To develop a convenient and sensitive point-of-care test for detecting gene mutations based on allele-specific PCR.
  
  
  RESULTS
  To develop a lateral flow strip for visual detection of K-ras mutations based on a modified PCR, a specific DNA tag was covalently linked to the 5'-end of each primer by a nine-carbon linker to produce a sticky end. One of the sticky ends of the PCR products bound to gold nano-particles, while the other sticky end was captured onto a nitrocellulose membrane of lateral flow strips. The lateral flow strip showed a great sensitivity, which detected mutations in as low as 10 tumor cells. The positive rate and accuracy of the lateral flow strip for blood samples were over 92 and 96 %, respectively.
  
  
  CONCLUSIONS
  The lateral flow strip provides an easy method for sensitive detection of gene mutations based on allele specific-PCR.
authors.affiliation: School of Pharmacy, Wenzhou Medical University, Wenzhou, 325030, Zhejiang, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Wenzhou Medical University, Wenzhou, 325030, Zhejiang, China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Chen
authors.affiliation: School of Pharmacy, Wenzhou Medical University, Wenzhou, 325030, Zhejiang, China.
authors.forename: Yuying
authors.initials: Y
authors.lastname: Wu
authors.affiliation: School of Pharmacy, Wenzhou Medical University, Wenzhou, 325030, Zhejiang, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.affiliation: School of Pharmacy, Wenzhou Medical University, Wenzhou, 325030, Zhejiang, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: The Third Affiliated Hospital of Wenzhou Medical University, Ruian, 325200, Zhejiang, China.
authors.forename: Xiaofu
authors.initials: X
authors.lastname: Pan
authors.affiliation: The Third Affiliated Hospital of Wenzhou Medical University, Ruian, 325200, Zhejiang, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Tang
authors.affiliation: School of Pharmacy, Wenzhou Medical University, Wenzhou, 325030, Zhejiang, China.
authors.forename: Ziying
authors.initials: Z
authors.lastname: Liu
authors.affiliation: School of Pharmacy, Wenzhou Medical University, Wenzhou, 325030, Zhejiang, China. xiaokunli@163.net.
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Li
mesh_terms: D000483:Alleles; D004247:DNA / Q000737:chemistry*; D006046:Gold; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry; D009154:Mutation; D019095:Point-of-Care Systems; D016133:Polymerase Chain Reaction / Q000379:methods*; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics*; D012680:Sensitivity and Specificity
chemical_list: C117307:KRAS protein, human; D006046:Gold; D004247:DNA; D016283:Proto-Oncogene Proteins p21(ras)
keywords: Detection of mutations; Gene mutation; K-ras mutations; Lateral flow strip; Mutation detection; Point-of-care tests