title: Chromosomal localisation of the CD4cre transgene in B6·Cg-Tg(Cd4-cre)1Cwi mice.
abstract: The B6·Cg-Tg(Cd4-cre)1Cwi line expresses CRE recombinase under the control of the promoter and regulatory elements of the Cd4 gene. Here we show that CRE recombinase expression reduces the number and frequencies of CD4 positive subsets in a dose-dependent manner and localize the integration site of the transgenic construct to position 60335693-60341285 (qD) of chromosome 3. The insert contains at least 15 complete sequential copies of the transgenic construct. Based on this information we describe a novel PCR assay for genetic typing of transgenic mice.
authors.affiliation: Deutsches Rheuma-Forschungszentrum Berlin, Chariteplatz 1, 10117 Berlin, Germany.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Westendorf
authors.affiliation: Deutsches Rheuma-Forschungszentrum Berlin, Chariteplatz 1, 10117 Berlin, Germany. Electronic address: pawel.durek@drfz.de.
authors.forename: Pawel
authors.initials: P
authors.lastname: Durek
authors.affiliation: Deutsches Rheuma-Forschungszentrum Berlin, Chariteplatz 1, 10117 Berlin, Germany.
authors.forename: Samia
authors.initials: S
authors.lastname: Ayew
authors.affiliation: Deutsches Rheuma-Forschungszentrum Berlin, Chariteplatz 1, 10117 Berlin, Germany. Electronic address: mashreghi@drfz.de.
authors.forename: Mir-Farzin
authors.initials: MF
authors.lastname: Mashreghi
authors.affiliation: Deutsches Rheuma-Forschungszentrum Berlin, Chariteplatz 1, 10117 Berlin, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Radbruch
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D005838:Genotype; D019426:Integrases / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D016133:Polymerase Chain Reaction / Q000379:methods*; D011401:Promoter Regions, Genetic; D019076:Transgenes
chemical_list: C045073:Cre recombinase; D019426:Integrases
keywords: CD4 cre; CRE recombinase; CRE toxicity; Genotyping; Transgene localisation; Whole genome sequencing