title: Differentially expressed transcripts in stomach of Penaeus monodon in response to AHPND infection.
abstract: Acute Hepatopancreatic Necrosis Disease (AHPND) is an emerging disease in aquacultured shrimp caused by a pathogenic strain of Vibrio parahaemolyticus. As with several pathogenic bacteria, colonization of the stomach appeared to be the initial step of the infection for AHPND-causing Vibrio. To understand the immune responses in the stomach of black tiger shrimp (Penaeus monodon), differentially expressed transcripts (DETs) in the stomach during V. parahaemolyticus strain 3HP (VP3HP) infection was examined using Ion Torrent sequencing. From the total 42,998 contigs obtained, 1585 contigs representing 1513 unigenes were significantly differentially expressed with 1122 and 391 unigenes up- and down-regulated, respectively. Among the DETs, there were 141 immune-related unigenes in 10 functional categories: antimicrobial peptide, signal transduction pathway, proPO system, oxidative stress, proteinases/proteinase inhibitors, apoptotic tumor-related protein, pathogen recognition immune regulator, blood clotting system, adhesive protein and heat shock protein. Expression profiles of 20 of 22 genes inferred from RNA sequencing were confirmed with the results from qRT-PCR. Additionally, a novel isoform of anti-lipopolysaccharide factor, PmALF7 whose transcript was induced in the stomach after challenge with VP3HP was discovered. This study provided a fundamental information on the molecular response in the shrimp stomach during the AHPND infection that would be beneficial for future research.
authors.affiliation: Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand. Electronic address: wipasiri39@hotmail.com.
authors.forename: Wipasiri
authors.initials: W
authors.lastname: Soonthornchai
authors.affiliation: Aquatic Molecular Genetics and Biotechnology Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Paholyothin Rd., Klong 1, Klong Luang, Pathumthani 12120, Thailand.
authors.forename: Sage
authors.initials: S
authors.lastname: Chaiyapechara
authors.affiliation: Aquatic Molecular Genetics and Biotechnology Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Paholyothin Rd., Klong 1, Klong Luang, Pathumthani 12120, Thailand.
authors.forename: Sirawut
authors.initials: S
authors.lastname: Klinbunga
authors.affiliation: School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36849, USA.
authors.forename: Wilawan
authors.initials: W
authors.lastname: Thongda
authors.affiliation: Genomic Research Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Paholyothin Rd., Klong 1, Klong Luang, Pathumthani 12120, Thailand.
authors.forename: Sithichoke
authors.initials: S
authors.lastname: Tangphatsornruang
authors.affiliation: Genomic Research Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Paholyothin Rd., Klong 1, Klong Luang, Pathumthani 12120, Thailand.
authors.forename: Thippawan
authors.initials: T
authors.lastname: Yoocha
authors.affiliation: Interdisciplinary Graduate Program on Maritime Administration, Graduate School, Chulalongkorn University, Bangkok 10330, Thailand. Electronic address: padermsak.j@chula.ac.th.
authors.forename: Padermsak
authors.initials: P
authors.lastname: Jarayabhand
authors.affiliation: Aquatic Molecular Genetics and Biotechnology Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Paholyothin Rd., Klong 1, Klong Luang, Pathumthani 12120, Thailand.
authors.forename: Pikul
authors.initials: P
authors.lastname: Jiravanichpaisal
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics* / Q000378:metabolism; D060829:Arthropod Proteins / Q000235:genetics; D007109:Immunity / Q000235:genetics; D008221:Lymphoid Tissue / Q000502:physiology*; D033561:Penaeidae / Q000276:immunology*; D020033:Protein Isoforms / Q000235:genetics; D017423:Sequence Analysis, RNA; D013270:Stomach / Q000502:physiology*; D059467:Transcriptome; D014735:Vibrio Infections / Q000276:immunology*; D014736:Vibrio parahaemolyticus / Q000276:immunology*
chemical_list: D023181:Antimicrobial Cationic Peptides; D060829:Arthropod Proteins; D020033:Protein Isoforms; C034705:antilipopolysaccharide factor (Limulus)
keywords: AHPND; Ion Torrent sequencing; Penaeus monodon; Stomach; Vibrio parahaemolyticus