title: Pseudomonas aeruginosa KUN2, extracellular toxins-A potential source for the control of dengue vector.
abstract: OBJECTIVE
  Dengue fever is one of the serious health disease transmitted by Aedes spp mosquitoes. The incidence of dengue has increased dramatically around the world in recent decades. Vector control is one of the important strategies practiced for the control of dengue fever. The emergence of resistance among vectors against the existing insecticides has raised new challenges. The aim of the present study was to identify the larvicidal activity of extracellular toxins from Pseudomonas spp for the control of dengue vector, Aedes aegypti.
  
  
  METHODS
  Bacterial isolates KUN1, KUN2, KUN3, KUN4, and KUBS were isolated from rhizosphere soil of the agricultural fields in Coimbatore, Tamil Nadu. Lyophilized culture supernatant of KUN2 (24, 48, and 72 h culture) and the solvent extracts from the diethyl ether, petroleum ether, chloroform and ethyl acetate were tested against the IV instar larvae of Ae. aegypti.
  
  
  RESULTS
  Morphological and biochemical characterization of KUN2 showed its resemblance to Pseudomonas spp. Further, characterization by molecular methods confirmed it as Pseudomonas aeruginosa. Lyophilized culture supernatant of KUN2 showed more toxicity towards the larvae of Ae. aegypti when grown in the modified medium. Secondary metabolite from the petroleum ether extract was found more toxic to the Ae. aegypti larvae even at low concentration (50 μg/ml). The supernatant of 48 h culture of KUN2 recorded 100% larvicidal activity when compared to other isolates. Further, the rate of mortality was 100% at 24 h when treated with 100 μg/ml of petroleum ether extract of KUN2.
  
  
  CONCLUSIONS
  Among the isolates used for the control of Ae. aegypti, the isolate KUN2 showed increased larvicidal activity when grown in the modified medium. The maximum larval mortality was observed in the solvent extract of petroleum ether. The mortality of the larvae might be due to the effect of the toxic compound present in the extract which would have entered the larvae through its cuticle damaging its whole system and obstructing further development. Further, studies on the toxic compound responsible for the larvicidal activity need to be carried out for effective dengue control.
authors.affiliation: Department of Biotechnology, School of Biotechnology and Health Sciences, Karunya University, Coimbatore, Tamil Nadu, India.
authors.forename: B
authors.initials: B
authors.lastname: Lalithambika
authors.affiliation: Department of Biotechnology, School of Biotechnology and Health Sciences, Karunya University, Coimbatore, Tamil Nadu, India.
authors.forename: C
authors.initials: C
authors.lastname: Vani
mesh_terms: D000330:Aedes / Q000187:drug effects* / Q000502:physiology; D000818:Animals; D001427:Bacterial Toxins / Q000378:metabolism*; D001431:Bacteriological Techniques; D001681:Biological Assay; D003715:Dengue / Q000517:prevention & control; D018562:Disease Transmission, Infectious / Q000517:prevention & control; D007194:India; D007306:Insecticides / Q000378:metabolism*; D007814:Larva / Q000187:drug effects / Q000502:physiology; D025202:Molecular Diagnostic Techniques; D009032:Mosquito Control / Q000379:methods; D000072138:Mosquito Vectors; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D012988:Soil Microbiology; D016019:Survival Analysis
chemical_list: D001427:Bacterial Toxins; D007306:Insecticides