title: [Preliminary Clinical Efficiency of Autologous Peripheral Blood Mononuclear Cells for Treating Critical Limb Ischemia of Thromboangiitis Obliterans].
abstract: OBJECTIVE
  To evaluate the long-term clinical effect of autologous peripheral blood mononuclear cells (PB-MNC) on critical limb ischemia (CLI) in patients with thromboangiitis obliterans (TAO) patients.
  
  
  METHODS
  The clinical data of 22 patients with CLI caused by TAO from July 2004 to May 2013 were analyzed retrospectively, 22 patients were divided into 2 groups; out of them 12 cases in one group were treated with granulocyte colony-stimulating factor (G-CSF)-mobilized autologous peripheral blood mononuclear cells (auto-PBMNC group), 10 cases in another group received conservative treatment (CT group). The log-rank test was used to compare the long-term outcomes in auto-PBMNC group and CT group.
  
  
  RESULTS
  The wound healing rate (P=0.016) and CLI-free rate (P=0.013) were significantly higher in PB-MNC group compared with that in CT group. No difference was found in amputation rates between the 2 groups (major amputation: P=0.361, minor and major amputation: P=0.867). No patients died or no serious adverse events occurred during the follow-up period.
  
  
  CONCLUSIONS
  The auto-PBMNC therapy can significantly promote the wound healing, and protect against CLI in TAO patients, but the risk of amputation is not low in comparison with conservative treatment.
authors.affiliation: Institute of Hematology & Hospital of Blood Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
authors.forename: Jing-Yi
authors.initials: JY
authors.lastname: Yu
authors.affiliation: Institute of Hematology & Hospital of Blood Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
authors.forename: Shang-Zhu
authors.initials: SZ
authors.lastname: Li
authors.affiliation: Institute of Hematology & Hospital of Blood Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Wu
authors.affiliation: Institute of Hematology & Hospital of Blood Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
authors.forename: Hong-Min
authors.initials: HM
authors.lastname: Li
authors.affiliation: Institute of Hematology & Hospital of Blood Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
authors.forename: Wen-Hui
authors.initials: WH
authors.lastname: Gao
authors.affiliation: Institute of Hematology & Hospital of Blood Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
authors.forename: Ya-Li
authors.initials: YL
authors.lastname: Zheng
authors.affiliation: Institute of Hematology & Hospital of Blood Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Xu
authors.affiliation: Institute of Hematology & Hospital of Blood Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
authors.forename: Qing-Guo
authors.initials: QG
authors.lastname: Liu
authors.affiliation: Institute of Hematology & Hospital of Blood Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
authors.forename: Jun-Fan
authors.initials: JF
authors.lastname: Li
authors.affiliation: Institute of Hematology & Hospital of Blood Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Liu
authors.affiliation: Institute of Hematology & Hospital of Blood Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.
authors.forename: Yi-Min
authors.initials: YM
authors.lastname: Hu
authors.affiliation: Institute of Hematology & Hospital of Blood Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China. E-mail: huangpp66@aliyun.com.
authors.forename: Ping-Ping
authors.initials: PP
authors.lastname: Huang
mesh_terms: D000671:Amputation, Surgical; D005121:Extremities / Q000503:physiopathology; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D007511:Ischemia / Q000628:therapy*; D007963:Leukocytes, Mononuclear / Q000637:transplantation*; D012189:Retrospective Studies; D013919:Thromboangiitis Obliterans / Q000628:therapy*; D014182:Transplantation, Autologous; D016896:Treatment Outcome; D014945:Wound Healing
chemical_list: D016179:Granulocyte Colony-Stimulating Factor