title: Protective Effect of Irisin on Atherosclerosis via Suppressing Oxidized Low Density Lipoprotein Induced Vascular Inflammation and Endothelial Dysfunction.
abstract: Irisin, a newly discovered myokine, is considered as a promising candidate for the treatment of metabolic disturbances and cardiovascular diseases. In the present study, we used two animal models, apolipoprotein E-deficient mice fed on a high-cholesterol diet and a mouse carotid partial ligation model to test the anti-atherosclerotic effect of irisin. Irisin treatment (0.5 μg/g body weight/day) significantly reduced the severity of aortic atherosclerosis in apolipoprotein E-deficient mice fed on a high-cholesterol diet and suppressed carotid neointima formation in a carotid partial ligation model. It was associated with decreased inflammation and cell apoptosis in aortic tissues. In addition, in a cell culture model, irisin restored ox-LDL-induced human umbilical vein endothelial cell dysfunction by reducing the levels of inflammatory genes via inhibiting the reactive oxygen species (ROS)/ p38 MAPK/ NF-κB signaling pathway activation and inhibiting cell apoptosis via up-regulating Bcl-2 and down-regulating Bax and caspase-3 expression. Our study demonstrated that irisin significantly reduced atherosclerosis in apolipoprotein E-deficient mice via suppressing ox-LDL-induced cell inflammation and apoptosis, which might have a direct therapeutic effect on atherosclerotic diseases.
authors.affiliation: Center for Stem Cell and Regenerative Medicine, The Second Hospital of Shandong University, Jinan, People's Republic of China.
authors.forename: Yuzhu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Center for Stem Cell and Regenerative Medicine, The Second Hospital of Shandong University, Jinan, People's Republic of China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Mu
authors.affiliation: Chaohu Road Community Health Center of Qingdao, Qingdao, People's Republic of China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Center for Stem Cell and Regenerative Medicine, The Second Hospital of Shandong University, Jinan, People's Republic of China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Song
authors.affiliation: Center for Stem Cell and Regenerative Medicine, The Second Hospital of Shandong University, Jinan, People's Republic of China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Center for Stem Cell and Regenerative Medicine, The Second Hospital of Shandong University, Jinan, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wu
authors.affiliation: Center for Stem Cell and Regenerative Medicine, The Second Hospital of Shandong University, Jinan, People's Republic of China.
authors.forename: Miao
authors.initials: M
authors.lastname: Jiang
authors.affiliation: Center for Stem Cell and Regenerative Medicine, The Second Hospital of Shandong University, Jinan, People's Republic of China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: Center for Stem Cell and Regenerative Medicine, The Second Hospital of Shandong University, Jinan, People's Republic of China.
authors.forename: Wen
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Center for Stem Cell and Regenerative Medicine, The Second Hospital of Shandong University, Jinan, People's Republic of China.
authors.forename: Liang
authors.initials: L
authors.lastname: Li
authors.affiliation: Center for Stem Cell and Regenerative Medicine, The Second Hospital of Shandong University, Jinan, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Shao
authors.affiliation: Key Laboratory of Cardiovascular Remodeling and Function Research, Qilu Hospital of Shandong University, Jinan, People's Republic of China.
authors.forename: Xingli
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Pathology, Immunology, and Laboratory Medicine, University of Florida College of Medicine, Gainesville, Florida, United States of America.
authors.forename: Shiwu
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Pathology, Immunology, and Laboratory Medicine, University of Florida College of Medicine, Gainesville, Florida, United States of America.
authors.forename: Lijun
authors.initials: L
authors.lastname: Yang
authors.affiliation: Department of Anatomy, School of Medicine Shandong University, Jinan, People's Republic of China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Key Laboratory of Cardiovascular Remodeling and Function Research, Qilu Hospital of Shandong University, Jinan, People's Republic of China.
authors.forename: Mingxiang
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Center for Stem Cell and Regenerative Medicine, The Second Hospital of Shandong University, Jinan, People's Republic of China.
authors.forename: Dongqi
authors.initials: D
authors.lastname: Tang
mesh_terms: D000818:Animals; D001011:Aorta / Q000473:pathology; D001057:Apolipoproteins E / Q000235:genetics; D017209:Apoptosis / Q000187:drug effects; D050197:Atherosclerosis / Q000378:metabolism / Q000517:prevention & control*; D002339:Carotid Arteries / Q000473:pathology; D053148:Caspase 3 / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D005353:Fibronectins / Q000494:pharmacology* / Q000502:physiology; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D007249:Inflammation / Q000517:prevention & control*; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008297:Male; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D000067996:RAW 264.7 Cells; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D001057:Apolipoproteins E; C577593:FNDC5 protein, human; D005353:Fibronectins; D008077:Lipoproteins, LDL; D016328:NF-kappa B; D017382:Reactive Oxygen Species; C099252:oxidized low density lipoprotein; D002784:Cholesterol; D053148:Caspase 3