title: Expression of Stipa purpurea SpCIPK26 in Arabidopsis thaliana Enhances Salt and Drought Tolerance and Regulates Abscisic Acid Signaling.
abstract: Stipa purpurea (S. purpurea) is the dominant plant species in the alpine steppe of the Qinghai-Tibet Plateau, China. It is highly resistant to cold and drought conditions. However, the underlying mechanisms regulating the stress tolerance are unknown. In this study, a CIPK gene from S. purpurea (SpCIPK26) was isolated. The SpCIPK26 coding region consisted of 1392 bp that encoded 464 amino acids. The protein has a highly conserved catalytic structure and regulatory domain. The expression of SpCIPK26 was induced by drought and salt stress. SpCIPK26 overexpression in Arabidopsis thaliana (A. thaliana) plants provided increased tolerance to drought and salt stress in an abscisic acid (ABA)-dependent manner. Compared with wild-type A. thaliana plants, SpCIPK26-overexpressing plants had higher survival rates, water potentials, and photosynthetic efficiency (Fv/Fm), as well as lower levels of reactive oxygen species (ROS) following exposure to drought and salt stress. Gene expression analyses indicated stress-inducible genes (RD29A, RD29B, and ABF2) and a ROS-scavenger gene (CAT1) were upregulated in SpCIPK26-overexpressing plants after stress treatments. All of these marker genes are associated with ABA-responsive cis-acting elements. Additionally, the similarities in the gene expression patterns following ABA, mannitol, and NaCl treatments suggest SpCIPK26 has an important role during plant responses to drought and salt stress and in regulating ABA signaling.
authors.affiliation: Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. zhouyanli@mail.kib.ac.cn.|Plant Germplasm and Genomics Center, the Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. zhouyanli@mail.kib.ac.cn.|University of Chinese Academy of Sciences, Beijing 100049, China. zhouyanli@mail.kib.ac.cn.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. sunxudong@mail.kib.ac.cn.|Plant Germplasm and Genomics Center, the Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. sunxudong@mail.kib.ac.cn.|Institute of Tibetan Plateau Research at Kunming, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. sunxudong@mail.kib.ac.cn.
authors.forename: Xudong
authors.initials: X
authors.lastname: Sun
authors.affiliation: Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. yangyunqiang@mail.kib.ac.cn.|Plant Germplasm and Genomics Center, the Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. yangyunqiang@mail.kib.ac.cn.|Institute of Tibetan Plateau Research at Kunming, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. yangyunqiang@mail.kib.ac.cn.
authors.forename: Yunqiang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. lixiong@mail.kib.ac.cn.|Plant Germplasm and Genomics Center, the Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. lixiong@mail.kib.ac.cn.|Institute of Tibetan Plateau Research at Kunming, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. lixiong@mail.kib.ac.cn.
authors.forename: Xiong
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Horticulture and Landscape, Yunnan Agricultural University, Kunming 650201, China. shidagonglao@gmail.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. yangyp@mail.kib.ac.cn.|Plant Germplasm and Genomics Center, the Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. yangyp@mail.kib.ac.cn.|University of Chinese Academy of Sciences, Beijing 100049, China. yangyp@mail.kib.ac.cn.|Institute of Tibetan Plateau Research at Kunming, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. yangyp@mail.kib.ac.cn.
authors.forename: Yongping
authors.initials: Y
authors.lastname: Yang
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D057773:Cold Shock Proteins and Peptides / Q000235:genetics / Q000378:metabolism; D017124:Conserved Sequence; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D006109:Poaceae / Q000235:genetics; D000072417:Protein Domains; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D055049:Salt Tolerance / Q000235:genetics*; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D019076:Transgenes; D015854:Up-Regulation; D050976:Basic-Leucine Zipper Transcription Factors
chemical_list: D029681:Arabidopsis Proteins; D002374:Catalase; D057773:Cold Shock Proteins and Peptides; D004268:DNA-Binding Proteins; D011494:Protein Kinases; D017382:Reactive Oxygen Species; D014157:Transcription Factors; C000595439:RD29B protein, Arabidopsis; C520629:RD29a protein, Arabidopsis; C584806:CIPK26 protein, Arabidopsis; C508877:At1g45249 protein, Arabidopsis; D050976:Basic-Leucine Zipper Transcription Factors
keywords: ABA; CIPK; ROS; Stipa purpurea; drought; salt