title: Gold nanoparticles covalently assembled onto vesicle structures as possible biosensing platform.
abstract: In this contribution a strategy is shown to covalently immobilize gold nanoparticles (AuNPs) onto vesicle bilayers with the aim of using this nanomaterial as platform for the future design of immunosensors. A novel methodology for the self-assembly of AuNPs onto large unilamellar vesicle structures is described. The vesicles were formed with 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1-undecanethiol (SH). After, the AuNPs photochemically synthesized in pure glycerol were mixed and anchored onto SH-DOPC vesicles. The data provided by voltammetry, spectrometry and microscopy techniques indicated that the AuNPs were successfully covalently anchored onto the vesicle bilayer and decorated vesicles exhibit a spherical shape with a size of 190 ± 10 nm. The developed procedure is easy, rapid and reproducible to start designing a possible immunosensor by using environmentally friendly procedures.
authors.affiliation: Departamento de Química. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Universidad Nacional de Río Cuarto. Agencia Postal Nº 3 - (5800) Río Cuarto, Argentina; REQUIMTE/LAQV, Instituto Superior de Engenharia do Porto, Instituto Politécnico do Porto, Rua Dr. António Bernardino de Almeida 431, 4200-072 Porto, Portugal.
authors.forename: M Fátima
authors.initials: MF
authors.lastname: Barroso
authors.affiliation: Departamento de Química. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Universidad Nacional de Río Cuarto. Agencia Postal Nº 3 - (5800) Río Cuarto, Argentina.
authors.forename: M Alejandra
authors.initials: MA
authors.lastname: Luna
authors.affiliation: Departamento de Química. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Universidad Nacional de Río Cuarto. Agencia Postal Nº 3 - (5800) Río Cuarto, Argentina.
authors.forename: Juan S Flores
authors.initials: JS
authors.lastname: Tabares
authors.affiliation: REQUIMTE/LAQV, Instituto Superior de Engenharia do Porto, Instituto Politécnico do Porto, Rua Dr. António Bernardino de Almeida 431, 4200-072 Porto, Portugal.
authors.forename: Cristina
authors.initials: C
authors.lastname: Delerue-Matos
authors.affiliation: Departamento de Química. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Universidad Nacional de Río Cuarto. Agencia Postal Nº 3 - (5800) Río Cuarto, Argentina.
authors.forename: N Mariano
authors.initials: NM
authors.lastname: Correa
authors.affiliation: Departamento de Química. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Universidad Nacional de Río Cuarto. Agencia Postal Nº 3 - (5800) Río Cuarto, Argentina.
authors.forename: Fernando
authors.initials: F
authors.lastname: Moyano
authors.affiliation: Departamento de Química. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Universidad Nacional de Río Cuarto. Agencia Postal Nº 3 - (5800) Río Cuarto, Argentina.
authors.forename: Patricia G
authors.initials: PG
authors.lastname: Molina
keywords: covalently decorated vesicles; gold nanoparticles; immunosensors design; synthesis