title: l-Histidine Induces Resistance in Plants to the Bacterial Pathogen Ralstonia solanacearum Partially Through the Activation of Ethylene Signaling.
abstract: Wilt disease in plants, which is caused by the soil-borne bacterial pathogen Ralstonia solanacearum, is one of the most devastating plant diseases. We previously detected bacterial wilt disease-inhibiting activity in an extract from yeast cells. In the present study, we purified this activity and identified one of the substances responsible for the activity as the amino acid histidine. The exogenous application of l-histidine, but not d-histidine, inhibited wilt disease in tomato and Arabidopsis plants without exhibiting any antibacterial activity. l-Histidine induced the expression of genes related to ethylene (ET) biosynthesis and signaling as well as the production of ET in tomato and Arabidopsis plants. l-Histidine-induced resistance to R. solanacearum was partially abolished in ein3-1, an ET-insensitive Arabidopsis mutant line. Resistance to the fungal pathogen Botrytis cinerea, which is known to require ET biosynthesis or signaling, was also induced by exogenously applied l-histidine. These results suggest that l-histidine induces resistance to R. solanacearum and B. cinerea partially through the activation of ET signaling in plants.
authors.affiliation: Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, 305-8602 Japan sseo71@affrc.go.jp nakaho@affrc.go.jp.
authors.forename: Shigemi
authors.initials: S
authors.lastname: Seo
authors.affiliation: Central Region Agricultural Research Center, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, 305-8666 Japan sseo71@affrc.go.jp nakaho@affrc.go.jp.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Nakaho
authors.affiliation: Plant-Microbe Research Units, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, 305-8602 Japan.
authors.forename: Si Won
authors.initials: SW
authors.lastname: Hong
authors.affiliation: Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, 981-8555 Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Takahashi
authors.affiliation: Graduate School of Life and Environmental Sciences, University of Tsukuba, Ibaraki, 305-8572 Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Shigemori
authors.affiliation: Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, 305-8602 Japan.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Mitsuhara
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000382:microbiology; D060467:Disease Resistance / Q000187:drug effects; D005030:Ethylenes / Q000378:metabolism*; D006639:Histidine / Q000494:pharmacology*; D018551:Solanum lycopersicum / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology*; D009154:Mutation; D010935:Plant Diseases / Q000382:microbiology*; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology; D043368:Ralstonia solanacearum / Q000472:pathogenicity*; D015398:Signal Transduction / Q000187:drug effects; D015003:Yeasts / Q000737:chemistry
chemical_list: D005030:Ethylenes; D006639:Histidine
keywords: Disease resistance; Ethylene; Plant activator; Ralstonia solanacearum; l-Histidine