title: DBA Lectin Binds to Highly Proliferative Mouse Erythroleukemia Cells.
abstract: OBJECTIVE
  Hematopoietic malignancies lead to disease states involving abnormal proliferation of blood cells. Ki-67 and carboxyfluorescein succinimidyl ester (CFSE) are assays used to examine the proliferation status of cells but affect cell viability. In this study, we used lectins to bind to surfaces of proliferating cells with different phenotypes while preserving cell viability.
  
  
  METHODS
  The mouse lymphocyte Friend leukemia F5-5.F1 cell line was stained using biotin-conjugated lectins from Canavalia ensiformis (ConA), Dolichos biflorus (DBA), Erythrina cristagalli (ECA), Lens culinaris (LCA), Phaseolus vulgaris (PHA-E4), Arachis hypogaea (PNA), Ulex europaeus (UEA) and Triticum vulgaris (WGA) and sorted by fluorescence-activated cell sorting. Morphology, gene expression and proliferation assays were performed on sorted cells.
  
  
  RESULTS
  DBA, LCA and PHA-E4 probing sorted cells based on surface phenotype. Gene expression analysis showed that myelocytomatosis oncogene (Myc), cyclin D1 (Ccnd1), and cyclinD2 (Ccnd2) were more highly expressed in the DBA(High) fraction than DBA(Int) and DBA(Neg) fractions. Ki-67 expression and MTS assay correlated with the DBA-binding pattern, with DBA(High) reflecting the highest proliferative tendency.
  
  
  CONCLUSIONS
  Labeling with DBA allows selection of proliferating cells using flow cytometry.
authors.affiliation: Department of Research and Development of Next Generation Medicine, Faculty of Medical Sciences, Kyushu University, Fukuoka, Japan Department of Cell Biology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan Center for Clinical and Translational Research, Kyushu University, Fukuoka, Japan.
authors.forename: Anthony
authors.initials: A
authors.lastname: Swain
authors.affiliation: Department of Research and Development of Next Generation Medicine, Faculty of Medical Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: Kasem
authors.initials: K
authors.lastname: Kulkeaw
authors.affiliation: Department of Cell Biology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Tanaka
authors.affiliation: Center for Clinical and Translational Research, Kyushu University, Fukuoka, Japan.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Nakanishi
authors.affiliation: Department of Cell Biology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan.
authors.forename: Senji
authors.initials: S
authors.lastname: Shirasawa
authors.affiliation: Department of Research and Development of Next Generation Medicine, Faculty of Medical Sciences, Kyushu University, Fukuoka, Japan Center for Clinical and Translational Research, Kyushu University, Fukuoka, Japan Center for Advanced Medical Innovation, Kyushu University, Fukuoka, Japan ds-mons@yb3.so-net.ne.jp.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Sugiyama
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004915:Leukemia, Erythroblastic, Acute; D051379:Mice; D037121:Plant Lectins / Q000737:chemistry*; D011485:Protein Binding; D013194:Staining and Labeling; D059467:Transcriptome
chemical_list: D037121:Plant Lectins; C036554:dolichos biflorus agglutinin
keywords: Lectin; cell proliferation; flow cytometry; leukemia