title: Symbiosis theory-directed green synthesis of silver nanoparticles and their application in infected wound healing.
abstract: In this study, silver nanoparticles (AgNPs) were synthesized for the first time using an antibacterial endophytic fungus of Chinese medicinal herb Orchidantha chinensis, which has anti-inflammatory and antimicrobial activities. The AgNPs were analyzed by various characterization techniques to reveal their morphology, chemical composition, and stability. Also, the relationship between Chinese medicinal herbs, endophytic fungi, and the property of AgNPs was investigated for the first time. Interestingly, an experiment performed in this study revealed the proteins produced by the endophytic fungus to be capped on the nanoparticles, which led to an increase in the stability of spherical and polydispersed AgNPs with low aggregation for over 6 months. More importantly, further study demonstrated that the AgNPs possessed superior antibacterial activity and effectively promoted wound healing. Altogether, the biosynthesis of active AgNPs using the endophytic fungus from Chinese medicinal herb based on the symbiosis theory is simple, eco-friendly, and promising.
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Guangzhou, People's Republic of China.
authors.forename: Lu
authors.initials: L
authors.lastname: Wen
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Guangzhou, People's Republic of China.
authors.forename: Pei
authors.initials: P
authors.lastname: Zeng
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Guangzhou, People's Republic of China.
authors.forename: Liping
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Guangzhou, People's Republic of China.
authors.forename: Wenli
authors.initials: W
authors.lastname: Huang
authors.affiliation: School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Guangzhou, People's Republic of China.
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015169:Colony Count, Microbial; D016207:Cytokines / Q000378:metabolism; D000067493:Dynamic Light Scattering; D055772:Green Chemistry Technology / Q000379:methods*; D006909:Hydroxyproline / Q000378:metabolism; D007249:Inflammation / Q000473:pathology; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D050997:Nanomedicine; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012834:Silver / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects; D014945:Wound Healing / Q000187:drug effects*; D014961:X-Ray Diffraction; D030521:Zingiberaceae / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D016207:Cytokines; D012834:Silver; D006909:Hydroxyproline
keywords: Orchidantha chinensis; endophytic fungi; silver nanoparticles; symbiosis theory; wound healing