title: Generation of a human iPSC line from a patient with Leigh syndrome.
abstract: Human iPSC line LND554SV.3 was generated from heteroplasmic fibroblasts of a patient with Leigh syndrome carrying a mutation in the MT-ND5 gene (m.13513GNA; p.D393N). Reprogramming factors Oct3/4, Sox2, Klf4,and cMyc were delivered using a non-integrative methodology that involves the use of Sendai virus.
authors.affiliation: Departamento de Bioquímica, Instituto de Investigaciones Biomédicas "Alberto Sols", Facultad de Medicina (UAM-CSIC), Spain; Centro de Investigación Biomédica en Red en Enfermedades Raras (CIBERER) Madrid, Spain; Instituto de Investigación Hospital 12 de Octubre ("i+12"), Madrid, Spain.
authors.forename: Teresa
authors.initials: T
authors.lastname: Galera
authors.affiliation: Departamento de Bioquímica, Instituto de Investigaciones Biomédicas "Alberto Sols", Facultad de Medicina (UAM-CSIC), Spain; Centro de Investigación Biomédica en Red en Enfermedades Raras (CIBERER) Madrid, Spain; Instituto de Investigación Hospital 12 de Octubre ("i+12"), Madrid, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Zurita
authors.affiliation: Departamento de Bioquímica, Instituto de Investigaciones Biomédicas "Alberto Sols", Facultad de Medicina (UAM-CSIC), Spain; Centro de Investigación Biomédica en Red en Enfermedades Raras (CIBERER) Madrid, Spain; Instituto de Investigación Hospital 12 de Octubre ("i+12"), Madrid, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: González-Páramos
authors.affiliation: Servicio de Genética, Hospital 12 de Octubre, Madrid, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Moreno-Izquierdo
authors.affiliation: Nanomaterials and Nanotechnology Research Center (CINN-CSIC), Spain.
authors.forename: Mario F
authors.initials: MF
authors.lastname: Fraga
authors.affiliation: Cancer Epigenetics Laboratory, Institute of Oncology of Asturias (IUOPA), HUCA, Universidad de Oviedo, Oviedo, Spain.
authors.forename: Agustin F
authors.initials: AF
authors.lastname: Fernández
authors.affiliation: Departamento de Bioquímica, Instituto de Investigaciones Biomédicas "Alberto Sols", Facultad de Medicina (UAM-CSIC), Spain; Centro de Investigación Biomédica en Red en Enfermedades Raras (CIBERER) Madrid, Spain; Instituto de Investigación Hospital 12 de Octubre ("i+12"), Madrid, Spain.
authors.forename: Rafael
authors.initials: R
authors.lastname: Garesse
authors.affiliation: Departamento de Bioquímica, Instituto de Investigaciones Biomédicas "Alberto Sols", Facultad de Medicina (UAM-CSIC), Spain; Centro de Investigación Biomédica en Red en Enfermedades Raras (CIBERER) Madrid, Spain; Instituto de Investigación Hospital 12 de Octubre ("i+12"), Madrid, Spain. Electronic address: egallardo@iib.uam.es.
authors.forename: M Esther
authors.initials: ME
authors.lastname: Gallardo
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002460:Cell Line; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D007621:Karyotyping; D000090062:Kruppel-Like Factor 4; D007888:Leigh Disease / Q000473:pathology*; D017422:Sequence Analysis, DNA
keywords: Induced pluripotent stem cells; Leigh syndrome; MT-ND5; iPS cells; iPSC