title: The effect of chemically modified electrospun silica nanofiber on the mRNA and miRNA expression profile of neural stem cell differentiation.
abstract: A detailed genomic and epigenomic analyses of neural stem cells (NSCs) differentiation in synthetic microenvironments is essential for the advancement of regenerative medicine and therapeutic treatment of diseases. This study identified the changes in mRNA and miRNA expression profile during NSC differentiation on an artificial matrix. NSCs were grown on a surface-modified, electrospun tetraethyl-orthosilicate nanofiber (designated as SNF-AP) by providing a 3D-environment for cell growth and differentiation. Differentially expressed mRNAs and miRNAs of NSC differentiated in this microenvironment were identified through microarray analysis. The genes and miRNA targets responsible for the differentiation fate of NSCs and neuron development process were determined using Ingenuity Pathway Analysis (IPA). SNF-AP enhanced the expression of genes that activates the proliferation, development, and outgrowth of neurons, differentiation and generation of cells, neuritogenesis, outgrowth of neurites, microtubule dynamics, formation of cellular protrusions, and long-term potentiation during NSC differentiation. On the other hand, PDL inhibited neuritogenesis, microtubule dynamics, and proliferation and differentiation of cells and activated the apoptosis function. Moreover, the nanomaterial promoted the expression of more let-7 miRNAs, which have vital roles in NSC differentiation. Overall, SNF-AP is biocompatible and applicable scaffold for NSC differentiation in the development of neural tissue engineering. These findings are useful in enhancing in vitro NSC differentiation potential for preclinical studies and future clinical applications. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2730-2743, 2016.
authors.affiliation: Department of Bioscience Technology, Chung Yuan Christian University, Chung-Li, 32023, Taiwan.|Department of Chemistry, Chung Yuan Christian University, Chung-Li, 32023, Taiwan.
authors.forename: Augustus T
authors.initials: AT
authors.lastname: Mercado
authors.affiliation: Department of Chemistry, Chung Yuan Christian University, Chung-Li, 32023, Taiwan.|Center for Biomedical Technology, Chung Yuan Christian University, Chung-Li, 32023, Taiwan.
authors.forename: Jui-Ming
authors.initials: JM
authors.lastname: Yeh
authors.affiliation: Department of Bioscience Technology, Chung Yuan Christian University, Chung-Li, 32023, Taiwan.|Center for Biomedical Technology, Chung Yuan Christian University, Chung-Li, 32023, Taiwan.
authors.forename: Ting Yu
authors.initials: TY
authors.lastname: Chin
authors.affiliation: Department of Chemistry, Chung Yuan Christian University, Chung-Li, 32023, Taiwan.|Center for Biomedical Technology, Chung Yuan Christian University, Chung-Li, 32023, Taiwan.
authors.forename: Wen Shuo
authors.initials: WS
authors.lastname: Chen
authors.affiliation: Department of Chemistry, Chung Yuan Christian University, Chung-Li, 32023, Taiwan.|Center for Biomedical Technology, Chung Yuan Christian University, Chung-Li, 32023, Taiwan.
authors.forename: Yui Whei
authors.initials: YW
authors.lastname: Chen-Yang
authors.affiliation: Department of Bioscience Technology, Chung Yuan Christian University, Chung-Li, 32023, Taiwan. cychen@cycu.edu.tw.|Center for Biomedical Technology, Chung Yuan Christian University, Chung-Li, 32023, Taiwan. cychen@cycu.edu.tw.
authors.forename: Chung-Yung
authors.initials: CY
authors.lastname: Chen
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D035683:MicroRNAs / Q000235:genetics*; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D058953:Neural Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D055495:Neurogenesis / Q000187:drug effects*; D012333:RNA, Messenger / Q000235:genetics*; D017207:Rats, Sprague-Dawley; D012822:Silicon Dioxide / Q000737:chemistry* / Q000494:pharmacology*; D059467:Transcriptome / Q000187:drug effects*
chemical_list: D001672:Biocompatible Materials; D035683:MicroRNAs; D012333:RNA, Messenger; D012822:Silicon Dioxide
keywords: NSC differentiation; expression profile; mRNA; miRNA; microarray; silica nanofiber