title: Effects of bavachin and its regulation of melanin synthesis in A375 cells.
abstract: The aim of the present study was to investigate the effect of bavachin treatment on A375 cells and the regulation of melanin synthesis. The cultured A375 cellsin vitrowere treated with bavachin; and the effect of bavachin on cell activity, tyrosinase (TYR) activity and melanin synthesis were respectively tested by the MTT assay, L-dopa oxidation assay and the NaOH lysis assay. The expression levels of TYR and c-Jun N-terminal kinases (JNK) proteins were tested by western blot analysis. The expression levels ofTYR, tyrosinase-related protein-1 (TRP-1),TRP-2, extracellular signal-regulated kinase 1 (ERK1),ERK2andJNK2mRNA were tested by the reverse transcription-polymerase chain reaction assay. Simultaneously, the effect of estrogen receptor inhibitor (ICI182780) and ERK pathway inhibitor (U0126) was also tested on A375 cells following bavachin. The safe dose of bavachin significantly inhibited melanin synthesis and TYR activity. Bavachin (10 µmol/l) inhibited the expression of TYR and JNK proteins, and the expression ofTYR,TRP-1,TRP-2,ERK1,ERK2andJNK2mRNA in A375 cells. ICI182780 and U0126 could significantly reverse the bavachin treatment on the protein expression levels and the mRNA expression ofTYR,TRP-1,TRP-2,ERK1,ERK2andJNK2. In conclusion, bavachin inhibited the synthesis of melanin on A375 cells by inhibiting the protein and mRNA expression ofTYR,TRP-1,TRP-2,ERK1,ERK2andJNK2.
authors.affiliation: College of Pharmacy, Mudanjiang Medical University, Mudanjiang, Heilongjiang 157011, P.R. China.
authors.forename: Jing-Hua
authors.initials: JH
authors.lastname: Wang
authors.affiliation: College of Pharmacy, Jiamusi University, Jiamusi, Heilongjiang 154007, P.R. China.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Pei
authors.affiliation: College of Jiamusi, Heilongjiang University of Chinese Medicine, Jiamusi, Heilongjiang 154007, P.R. China.
authors.forename: Hong-Dan
authors.initials: HD
authors.lastname: Xu
authors.affiliation: College of Pharmacy, Changchun University of Chinese Medicine, Changchun, Jilin 130117, P.R. China.
authors.forename: Li-Jing
authors.initials: LJ
authors.lastname: Li
authors.affiliation: College of Jiamusi, Heilongjiang University of Chinese Medicine, Jiamusi, Heilongjiang 154007, P.R. China.
authors.forename: Ye-Qiu
authors.initials: YQ
authors.lastname: Wang
authors.affiliation: College of Jiamusi, Heilongjiang University of Chinese Medicine, Jiamusi, Heilongjiang 154007, P.R. China.
authors.forename: Guo-Liang
authors.initials: GL
authors.lastname: Liu
authors.affiliation: College of Jiamusi, Heilongjiang University of Chinese Medicine, Jiamusi, Heilongjiang 154007, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Qu
authors.affiliation: College of Jiamusi, Heilongjiang University of Chinese Medicine, Jiamusi, Heilongjiang 154007, P.R. China.
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
keywords: A375 cells; bavachin; estrogen receptor-mitogen-activated protein kinase; melanin