title: Expression of a Sensory Neuron Membrane Protein SNMP2 in Olfactory Sensilla of Codling Moth Cydia pomonella (Lepidoptera: Tortricidae).
abstract: In insects, sensory neuron membrane proteins (SNMPs) are critical peripheral olfactory proteins and highly promote the sensitivity of pheromone detection. In this study, we cloned an SNMP transcript (CpomSNMP2, GenBank KU302714) from the antennae of the codling moth Cydia pomonella (L.) Its open reading frame is 1,575 bp and it encodes a protein with 524 amino acids. CpomSNMP2 contains two putative transmembrane domains and has a large extracellular loop. Phylogenetic analysis showed that CpomSNMP2 is clustered into the group of previously characterized lepidopteron SNMP2s. Expression levels of CpomSNMP2 were significantly higher in antennae of both males and females than in tissues from the thoraxes, abdomens, legs, and wings. CpomSNMP2 was distributed in sensilla trichodea of both males and females, but only in sensilla chaetica of males. This study provides evidence for olfactory roles of CpomSNMP2 in this moth.
authors.affiliation: Key Laboratory of Plant Protection Resources & Pest Management of the Ministry of Education, Northwest A&F University, Yangling 712100, Shaanxi, China (hxl4645@163.com; liulu_nwsuaf@163.com; missroad@126.com; jinianf@nwsuaf.edu.cn).
authors.forename: Xinglong
authors.initials: X
authors.lastname: Huang
authors.affiliation: Key Laboratory of Plant Protection Resources & Pest Management of the Ministry of Education, Northwest A&F University, Yangling 712100, Shaanxi, China (hxl4645@163.com; liulu_nwsuaf@163.com; missroad@126.com; jinianf@nwsuaf.edu.cn).
authors.forename: Lu
authors.initials: L
authors.lastname: Liu
authors.affiliation: Key Laboratory of Plant Protection Resources & Pest Management of the Ministry of Education, Northwest A&F University, Yangling 712100, Shaanxi, China (hxl4645@163.com; liulu_nwsuaf@163.com; missroad@126.com; jinianf@nwsuaf.edu.cn).
authors.forename: Yiqing
authors.initials: Y
authors.lastname: Fang
authors.affiliation: Key Laboratory of Plant Protection Resources & Pest Management of the Ministry of Education, Northwest A&F University, Yangling 712100, Shaanxi, China (hxl4645@163.com; liulu_nwsuaf@163.com; missroad@126.com; jinianf@nwsuaf.edu.cn), jinianf@nwsuaf.edu.cn.
authors.forename: Jinian
authors.initials: J
authors.lastname: Feng
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D005260:Female; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D009036:Moths / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D058618:Sensilla / Q000378:metabolism*; D011984:Sensory Receptor Cells / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D018076:DNA, Complementary; D019476:Insect Proteins; D008565:Membrane Proteins
keywords: Cydia pomonella; antenna; immunocytochemistry; olfactory; sensory neuron membrane protein