title: [Metabolites Identification for Alkaloids from Nelumbinis Plumula in Caco-2 Cells by LC/MS/MS].
abstract: OBJECTIVE
  To identify the metabolites of norcoclaurine,liensinine, isoliensinine and neferine in Caco-2 cells by LC/ MS/MS.
  
  
  METHODS
  After Caco-2 cells were treated with norcoclaurine, liensinine, isoliensinine or neferine for 3, 6 and 12 h, samples were collected, purified and then analyzed by LC/MS/MS. The structures of the metabolites were elucidated by molecular masses, retention times, MS and MS/MS spectra comparing with those of the parent drug.
  
  
  RESULTS
  The procedure identified that the major metabolites of norcoclaurine were methylnorcoclaurine and norcoclaurine-glucuronide, the major metabolite of liensinine was demethyl-liensinine, the major metabolite of isoliensinine was demethyl-isoliensinine, the major metabolites of neferine were liensinine, isoliensinine and their further demethylation products.
  
  
  CONCLUSIONS
  LC/MS/MS is simple, rapid and sensitive for the metabolites identification. Methylation, demethylation and glucuronidation are main metabolic pathways of alkaloids from Nelumbinis Plumula in Caco-2 cells.
authors.forename: Min-ting
authors.initials: MT
authors.lastname: Lin
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.forename: Si-li
authors.initials: SL
authors.lastname: Tang
authors.forename: Zhong-zhen
authors.initials: ZZ
authors.lastname: Zhao
authors.forename: Hu-biao
authors.initials: HB
authors.lastname: Chen
authors.forename: Jian-ye
authors.initials: JY
authors.lastname: Zhang
mesh_terms: D000470:Alkaloids / Q000378:metabolism*; D044182:Benzylisoquinolines / Q000378:metabolism; D018938:Caco-2 Cells; D002853:Chromatography, Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D006801:Humans; D007546:Isoquinolines / Q000378:metabolism; D019684:Magnoliopsida / Q000737:chemistry*; D053858:Metabolic Networks and Pathways; D008745:Methylation; D008970:Molecular Weight; D010636:Phenols / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D044005:Tetrahydroisoquinolines / Q000378:metabolism
chemical_list: D000470:Alkaloids; D044182:Benzylisoquinolines; D004365:Drugs, Chinese Herbal; D007546:Isoquinolines; D010636:Phenols; D044005:Tetrahydroisoquinolines; C499779:isoliensinine; C057222:neferine; C080095:liensinine; C012348:higenamine