title: [IMPACT OF ACTIVE OXYGEN FORMS INDUCTION ON EXPRESSION OF MOLECULAR ADHESION AND PARACRINE ACTIVITY OF MESENCHIMAL STROMAL CELLS].
abstract: Impact of low doses of active oxygen forms (AOFs) on the paracrine activity of mesenchimal stromal cells (MSCs) was studied. Photodynamic treatment (PDT) was shown to be a method for controlled generation of intracellular AOFs. Active oxygen forms generated at a dose of 0.25J/cm² do not impact significantly the MSCs mitochondrial activity or viability and can be recognized as regulatory. This was the first discovery that low-intensity PDT modulates substantially the MSCs paracrine activity which was manifested by an increased secretion of proinflammatory cytokines (IL-6, IL-8), vascular endothelium growth factor (VEGF), and suppressed secretion of the transforming growth factor (TGFβ). While expression of intercellular adhesion molecule ICAM-1 increases and of Thy-1 antigen (a common MSCs marker) decreases, no changes occur to expression of chemokine receptor CXCR4 or other adhesion molecules (H-CAM, MCAM and VCAM-1). Our data make it clear that low-dose PDT is the most important regulator of the MSCs function. Key words: active oxygen forms, mesenchimal stromal cells, paracrine activity, photodynamic exposure.
authors.forename: O O
authors.initials: OO
authors.lastname: Udartseva
authors.forename: E R
authors.initials: ER
authors.lastname: Andreeva
authors.forename: L B
authors.initials: LB
authors.lastname: Buravkova
mesh_terms: D002470:Cell Survival / Q000235:genetics; D005786:Gene Expression Regulation / Q000528:radiation effects; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D016209:Interleukin-8 / Q000096:biosynthesis; D007834:Lasers; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism* / Q000528:radiation effects; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics / Q000528:radiation effects; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis
chemical_list: D015850:Interleukin-6; D016209:Interleukin-8; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1