title: Downregulation of stathmin 1 in human gallbladder carcinoma inhibits tumor growth in vitro and in vivo.
abstract: Gallbladder carcinoma (GBC) is a highly lethal malignancy of the gastrointestinal tract. Despite extensive research, the underlying molecular mechanism of GBC remains largely unclear. Stathmin 1 (STMN1) is an important cytosolic protein associated with microtubule stability that was reported to be involved in tumorigenesis. Up to our knowledge, its role in gallbladder carcinoma has not been analyzed. In this study, we found that STMN1 was significantly highly expressed in GBC by immunohistochemistry (IHC). Further research demonstrated that silencing of STMN1 inhibited cell growth in vitro. Moreover, knockdown of STMN1 induced apoptosis and delayed G2/M phase transformation in GBC cells. Our data support a rationale for further studies that the silencing of STMN1 may regulate the activity of p38 MAPK kinase and p53/p21 signal pathway. Besides, xenografted gallbladder carcinoma cells growth were significantly impaired after STMN1 was silenced in vivo. These results suggested that STMN1 played an important role in cell proliferation and migration. This provided a potential clue for investigating the therapeutic target in GBC.
authors.affiliation: Department of General Surgery, Zhongshan Hospital, General Surgery Institute, Fudan University, Shanghai 200030, China.|Glycobiology and Glycochemistry Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
authors.forename: Jiwen
authors.initials: J
authors.lastname: Wang
authors.affiliation: Glycobiology and Glycochemistry Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Yao
authors.affiliation: PET-CT Center, Cancer Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100021, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Ming
authors.affiliation: Department of General Surgery, Zhongshan Hospital, General Surgery Institute, Fudan University, Shanghai 200030, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Shen
authors.affiliation: Beijing Key Laboratory for Genetic Research of Skeletal Deformity, Beijing, China.|Department of Orthopaedic Surgery, Peking Union Medical College Hospital, Peking Union Medical College; Chinese Academy of Medical Sciences, Beijing 100730, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Wu
authors.affiliation: Department of Breast Surgical Oncology, Cancer Hospital of Chinese Academy of Medical Sciences, Beijing, 100021, P. R. China.
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of General Surgery, Zhongshan Hospital, General Surgery Institute, Fudan University, Shanghai 200030, China.
authors.forename: Han
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of General Surgery, Zhongshan Hospital, General Surgery Institute, Fudan University, Shanghai 200030, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Suo
authors.affiliation: Department of General Surgery, Zhongshan Hospital, General Surgery Institute, Fudan University, Shanghai 200030, China.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Pan
authors.affiliation: Department of General Surgery, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai 200240, China.
authors.forename: Dexiang
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Glycobiology and Glycochemistry Lab, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
authors.forename: Kan
authors.initials: K
authors.lastname: Ding
authors.affiliation: Department of General Surgery, Zhongshan Hospital, General Surgery Institute, Fudan University, Shanghai 200030, China.
authors.forename: Houbao
authors.initials: H
authors.lastname: Liu
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D015536:Down-Regulation; D005260:Female; D005706:Gallbladder Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000628:therapy; D006801:Humans; D007150:Immunohistochemistry; D008807:Mice, Inbred BALB C; D034622:RNA Interference; D000067492:RNAi Therapeutics / Q000379:methods; D050777:Stathmin / Q000096:biosynthesis / Q000235:genetics*; D047368:Tumor Burden / Q000235:genetics; D023041:Xenograft Model Antitumor Assays / Q000379:methods*
chemical_list: C493995:STMN1 protein, human; D050777:Stathmin