title: Immunotherapy based on dendritic cells pulsed with CTPFoxM1 fusion protein protects against the development of hepatocellular carcinoma.
abstract: Application of dendritic cells (DCs) pulsed with tumor-associated antigens is considered attractive in immunotherapy for hepatocellular carcinoma (HCC). In order to efficiently prime tumor-associated antigens specific for cytotoxic T lymphocytes (CTLs), it is important that DCs present tumor-associated antigens on MHC class I. MHC class I generally present endogenous antigens expressed in the cytosol. In this study, we developed a new antigen delivery tool based on cross presentation of exogenous antigens in DCs by using cytoplasmic transduction peptide (CTP). CTP protein could transduce FoxM1 tumor antigen into the cytosol of DCs, and CTP-FoxM1 fusion protein could stimulate activation and maturation of DCs. DCs pulsed with CTP-FoxM1 could induce specific CTLs. More importantly, the immunity induced by DCs loaded with CTP-FoxM1 could significantly inhibit tumor growth and metastasis in HCC-bearing mice, which was more potent than that induced by DCs loaded with FoxM1 or CTP, alone. Our results indicate that DCs pulsed with CTP-FoxM1 might be a promising vaccine candidate for HCC therapy and provide new insight into the design of DC-based immunotherapy.
authors.affiliation: Department of Laboratory Medicine, The First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Huiting
authors.initials: H
authors.lastname: Su
authors.affiliation: Department of Laboratory Medicine, The First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Laboratory Medicine, The First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Department of Laboratory Medicine, The First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Haixia
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Laboratory Medicine, The First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D000951:Antigens, Neoplasm / Q000235:genetics / Q000276:immunology* / Q000627:therapeutic use; D006528:Carcinoma, Hepatocellular / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D045744:Cell Line, Tumor; D003600:Cytosol / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology* / Q000637:transplantation; D005260:Female; D000072278:Forkhead Box Protein M1 / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use; D005805:Genes, MHC Class I / Q000276:immunology; D016219:Immunotherapy, Adoptive / Q000379:methods*; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000627:therapeutic use; D008113:Liver Neoplasms / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D051379:Mice; D008810:Mice, Inbred C57BL; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology* / Q000627:therapeutic use; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D000951:Antigens, Neoplasm; D000072278:Forkhead Box Protein M1; C491524:Foxm1 protein, mouse; D047908:Intracellular Signaling Peptides and Proteins; D011993:Recombinant Fusion Proteins
keywords: FoxM1; cytoplasmic transduction peptide; dendritic cells; hepatocellular carcinoma; immunotherapy