title: Anti-gliomas Effect of Chlorotoxin-Conjugated Onconase at High Dose.
abstract: Malignant gliomas are rarely curable malignant tumors in the central nervous system. Chlorotoxin (CTX) is a peptide derived from scorpion venom, which can selectively target malignant gliomas. Onconase (Onc) is a small cytotoxic ribonuclease derived from frogspawn that exhibits cytotoxicity against some tumor cells. In the present study, we found that CTX-conjugated Onc (CTX-Onc) shows better anti-tumor effect than the physical mixture of CTX and Onc (CTX + Onc) on the nude mice carrying subcutaneous glioblastoma cell-derived tumor. However, CTX-Onc does not show dose-dependent anti-tumor effect. In addition, apoptosis in tumor tissue does not show significant difference between the treatment groups. Our results confirmed that CTX-Onc has better anti-tumor effect than CTX + Onc and suggest that it can be potentially used for glioma therapy.
authors.affiliation: Institute of Protein Research, College of Life Sciences and Technology, Tongji University, Shanghai, 200092, China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Institute of Protein Research, College of Life Sciences and Technology, Tongji University, Shanghai, 200092, China. guo_zhanyun1@163.com.
authors.forename: Zhanyun
authors.initials: Z
authors.lastname: Guo
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000627:therapeutic use / Q000633:toxicity*; D001932:Brain Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D005910:Glioma / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008856:Microscopy, Fluorescence; D012260:Ribonucleases / Q000737:chemistry*; D012604:Scorpion Venoms / Q000737:chemistry*; D014183:Transplantation, Heterologous
chemical_list: D000970:Antineoplastic Agents; D012604:Scorpion Venoms; C091539:Chlorotoxin; D012260:Ribonucleases; C058703:ranpirnase
keywords: Chemical conjugation; Chlorotoxin; Gliomas; Onconase; Targeted therapy