title: Respiratory syncytial virus subtype ON1/NA1/BA9 predominates in hospitalized children with lower respiratory tract infections.
abstract: Respiratory syncytial virus (RSV) infection is the leading cause of acute respiratory tract disease in children less than 5 years old. The aim of this study was to further elucidate the molecular properties and clinical characteristics of RSV infection. The study sample included 238 patients <5 years old who were hospitalized with clinical symptoms of upper or lower respiratory tract infection (URTI or LRTI) in the Pediatric Department at the First People's Hospital of Chenzhou, South China in 2014. We subjected nasopharyngeal aspirate (NPA) or nasal swab (NS) samples from the patients to indirect fluorescence assay screens. RSV G genes were amplified by reverse transcription-PCR (RT-PCR) and sequenced. Of the 238 patients screened, 64 (26.8%) were confirmed to have RSV infections. Of those 64 confirmed RSV infection cases, 39 (60.9%) had subtype BA9, 13 (20.3%) had the recently identified subtype ON1, 11 (17.2%) had subtype NA1, and 1 (1.6%) had subtype GB2. The predominant presentation was LRTI with coughing, sputum production, fever, and wheezing. RSV subtype NA1 and BA9 infections were found mostly in infants, whereas the age distribution of subtype ON1 infections was more uniform across the age bands. Phylogenetic analysis indicated that, compared with the prototype strain A2, all ON1 and most NA1 isolates had lost one potential N-glycosylation site at amino acid 251 and 249 due to T251K and N249Y substitution, respectively. These findings suggest that NA1, BA9, and ON1 are the dominant RSV subtypes causing respiratory tract infections in young children presenting to the hospital in South China. J. Med. Virol. 89:213-221, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Translational Medicine Institute, National and Local Joint Engineering Laboratory for High-Throughput Molecular Diagnosis Technology, Affiliated The First People's Hospital of Chenzhou, University of South China, Chenzhou, People's Republic of China.|Department of Pediatric, Guangzhou Women and Children's Medical Center, Guangzhou, People's Republic of China.
authors.forename: Ruyan
authors.initials: R
authors.lastname: Fan
authors.affiliation: Translational Medicine Institute, National and Local Joint Engineering Laboratory for High-Throughput Molecular Diagnosis Technology, Affiliated The First People's Hospital of Chenzhou, University of South China, Chenzhou, People's Republic of China.
authors.forename: Chuping
authors.initials: C
authors.lastname: Fan
authors.affiliation: Translational Medicine Institute, National and Local Joint Engineering Laboratory for High-Throughput Molecular Diagnosis Technology, Affiliated The First People's Hospital of Chenzhou, University of South China, Chenzhou, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Translational Medicine Institute, National and Local Joint Engineering Laboratory for High-Throughput Molecular Diagnosis Technology, Affiliated The First People's Hospital of Chenzhou, University of South China, Chenzhou, People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wen
authors.affiliation: Translational Medicine Institute, National and Local Joint Engineering Laboratory for High-Throughput Molecular Diagnosis Technology, Affiliated The First People's Hospital of Chenzhou, University of South China, Chenzhou, People's Republic of China.
authors.forename: Yefei
authors.initials: Y
authors.lastname: Lei
authors.affiliation: Translational Medicine Institute, National and Local Joint Engineering Laboratory for High-Throughput Molecular Diagnosis Technology, Affiliated The First People's Hospital of Chenzhou, University of South China, Chenzhou, People's Republic of China.
authors.forename: Chan
authors.initials: C
authors.lastname: Liu
authors.affiliation: Translational Medicine Institute, National and Local Joint Engineering Laboratory for High-Throughput Molecular Diagnosis Technology, Affiliated The First People's Hospital of Chenzhou, University of South China, Chenzhou, People's Republic of China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Chen
authors.affiliation: Translational Medicine Institute, National and Local Joint Engineering Laboratory for High-Throughput Molecular Diagnosis Technology, Affiliated The First People's Hospital of Chenzhou, University of South China, Chenzhou, People's Republic of China.
authors.forename: Wenpei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Public Health, Sichuan University, Chengdu, People's Republic of China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Wang
authors.affiliation: Translational Medicine Institute, National and Local Joint Engineering Laboratory for High-Throughput Molecular Diagnosis Technology, Affiliated The First People's Hospital of Chenzhou, University of South China, Chenzhou, People's Republic of China.
authors.forename: Xiaowang
authors.initials: X
authors.lastname: Qu
mesh_terms: D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D005260:Female; D005838:Genotype; D006760:Hospitalization; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D017720:Molecular Epidemiology; D009296:Nasal Cavity / Q000821:virology; D009305:Nasopharynx / Q000821:virology; D010802:Phylogeny; D011024:Pneumonia, Viral / Q000453:epidemiology / Q000473:pathology / Q000821:virology*; D015995:Prevalence; D018357:Respiratory Syncytial Virus Infections / Q000453:epidemiology / Q000473:pathology / Q000821:virology*; D012136:Respiratory Syncytial Viruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012141:Respiratory Tract Infections / Q000453:epidemiology / Q000473:pathology / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014760:Viral Fusion Proteins / Q000235:genetics
chemical_list: C502275:G glycoprotein, Respiratory syncytial virus; D014760:Viral Fusion Proteins
keywords: attachment glycoprotein (G) gene; lower respiratory tract infections (LRTIs); respiratory syncytial virus (RSV; subtype BA9; subtype NA1; subtype ON1