title: Regulatory effect of miRNA on multi-directional differentiation ability of mesenchymal stem cell in treatment of osteoporosis.
abstract: This study was designed to evaluate the effect of miRNA acting in regulating multi-directional differentiation ability of mesenchymal stem cell in treatment of osteoporosis (OP), with the aim of finding a new idea and approach for clinical treatment of OP. Estrogen deficiency-induced OP mice model was established by means of ovariectomy (OVX). Additionally, a sham group was set up for control. Bone Marrow Mesenchymal Stem Cells (BMMSCs) of OVX group (O/BMMSCs) and BMMSCs of sham group (S/BMMSCs) were separately cultured. Then surface markers of BMMSCs were detected. Multi-directional differentiation ability was identified in the two groups by giving cells targeted induced stimulation. It was found that the bone trabecula, bone density and bone volume fraction of distal femoral metaphysis in the OVX group were much lower than those of the sham group. Moreover, trabecular bone space in the OVX group became larger; O/BMMSCs and S/BMMSCs both had normal expression of surface markers as well as potentials of osteogenic and adipogenic differentiation; O/BMMSCs had a weaker osteogenic capability but a stronger adipogenic capability than S/BMMSCs. All the findings suggest that the regulatory effect of miRNA on multi-directional differentiation ability plays a vital role in the treatment of OP, and there is a close correlation between them; deficiency or functional defect of BMMSCs can result in the occurrence of OP.
authors.affiliation: Department of Orthopedic Surgery, The Peoples Hospital of Yuxi City, The 6th Affiliated Hospital of Kunming Medical University, Yuxi, Yunnan, China.
authors.forename: Z W
authors.initials: ZW
authors.lastname: Teng
authors.affiliation: Department of Nephrology, The Peoples Hospital of Yuxi City, The 6th Affiliated Hospital of Kunming Medical University, Yuxi, Yunnan, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Orthopedic Surgery, The Peoples Hospital of Yuxi City, The 6th Affiliated Hospital of Kunming Medical University, Yuxi, Yunnan, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Na
authors.affiliation: Department of Orthopedic Surgery, The Peoples Hospital of Yuxi City, The 6th Affiliated Hospital of Kunming Medical University, Yuxi, Yunnan, China.
authors.forename: X G
authors.initials: XG
authors.lastname: Zhang
authors.affiliation: Department of Orthopedic Surgery, The Peoples Hospital of Yuxi City, The 6th Affiliated Hospital of Kunming Medical University, Yuxi, Yunnan, China.
authors.forename: H
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Orthopedic, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China.
authors.forename: G J
authors.initials: GJ
authors.lastname: Wei
authors.affiliation: Department of Orthopedic Surgery, The Peoples Hospital of Yuxi City, The 6th Affiliated Hospital of Kunming Medical University, Yuxi, Yunnan, China.
authors.forename: J
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Orthopedic Surgery, The Peoples Hospital of Yuxi City, The 6th Affiliated Hospital of Kunming Medical University, Yuxi, Yunnan, China.
authors.forename: X W
authors.initials: XW
authors.lastname: Zhang
authors.affiliation: Department of Orthopedic Surgery, The Peoples Hospital of Yuxi City, The 6th Affiliated Hospital of Kunming Medical University, Yuxi, Yunnan, China.
authors.forename: G Z
authors.initials: GZ
authors.lastname: Wang
authors.affiliation: Department of Orthopedic Surgery, The Peoples Hospital of Yuxi City, The 6th Affiliated Hospital of Kunming Medical University, Yuxi, Yunnan, China.
authors.forename: J
authors.initials: J
authors.lastname: Liang
authors.affiliation: Department of Orthopedic Surgery, General Hospital of Jizhong Energy Fengfeng Group, Handan, Hebei, China.
authors.forename: Z J
authors.initials: ZJ
authors.lastname: Zhang
authors.affiliation: Department of Orthopedic Surgery, Affiliated Hospital of Hebei University of Engineering, Handan, Hebei, China.
authors.forename: Y G
authors.initials: YG
authors.lastname: Liu
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D005260:Female; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000502:physiology*; D010012:Osteogenesis; D010024:Osteoporosis / Q000628:therapy*
chemical_list: D035683:MicroRNAs