title: Albumin microvascular leakage in brains with diabetes mellitus.
abstract: Their aim was to examine whether microvascular leakage of endogenous albumin, a representative marker for blood-brain barrier (BBB) damage, was induced in the periventricular area of diabetic db/db mice because periventricular white matter hyperintensity formation in magnetic resonance images was accelerating in elderly patients with diabetes mellitus. Using light and electron microscopes, and semi-quantitative analysis techniques, immunoreactivity of endogenous albumin, indicating vascular permeability, was examined in the periventricular area and spinal cord of db/db mice and db/+m control mice. Greater immunoreactivity of albumin was observed in the vessel wall of the periventricular area of db/db mice than in controls. Additionally, weak immunoreactivity was observed in the spinal cord of both db/db mice and controls. The number of gold particles, indicating immunoreactivity of albumin, in the perivascular area of db/db mice was significantly higher than that of control mice, but there was no significant difference in the number of particles in the spinal cord between db/db mice and controls. These findings suggest that albumin microvascular leakage, or BBB breakdown, is induced in the periventricular area of diabetic mice. Microsc. Res. Tech. 79:833-837, 2016. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Pathology and Host Defense, , Faculty of Medicine, Kagawa University, Kagawa, 761-0793, Japan.|Department of Orthopaedic Surgery, Faculty of Medicine, Kagawa University, Kagawa, 761-0793, Japan.
authors.forename: Ryuji
authors.initials: R
authors.lastname: Fujihara
authors.affiliation: Department of Pathology and Host Defense, , Faculty of Medicine, Kagawa University, Kagawa, 761-0793, Japan.
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Chiba
authors.affiliation: Life Science Research Center, Faculty of Medicine, Kagawa University, Kagawa, 761-0793, Japan.
authors.forename: Toshitaka
authors.initials: T
authors.lastname: Nakagawa
authors.affiliation: Life Science Research Center, Faculty of Medicine, Kagawa University, Kagawa, 761-0793, Japan.
authors.forename: Nozomu
authors.initials: N
authors.lastname: Nishi
authors.affiliation: Department of Pathology and Host Defense, , Faculty of Medicine, Kagawa University, Kagawa, 761-0793, Japan.
authors.forename: Ryuta
authors.initials: R
authors.lastname: Murakami
authors.affiliation: Department of Pathology and Host Defense, , Faculty of Medicine, Kagawa University, Kagawa, 761-0793, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Matsumoto
authors.affiliation: Department of Pathology and Host Defense, , Faculty of Medicine, Kagawa University, Kagawa, 761-0793, Japan.
authors.forename: Machi
authors.initials: M
authors.lastname: Kawauchi
authors.affiliation: Department of Orthopaedic Surgery, Faculty of Medicine, Kagawa University, Kagawa, 761-0793, Japan.
authors.forename: Tetsuji
authors.initials: T
authors.lastname: Yamamoto
authors.affiliation: Department of Pathology and Host Defense, , Faculty of Medicine, Kagawa University, Kagawa, 761-0793, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Ueno
mesh_terms: D000418:Albumins / Q000032:analysis* / Q000737:chemistry; D000818:Animals; D001812:Blood-Brain Barrier / Q000000981:diagnostic imaging* / Q000648:ultrastructure*; D001921:Brain / Q000000981:diagnostic imaging* / Q000648:ultrastructure*; D001923:Brain Chemistry; D003921:Diabetes Mellitus, Experimental / Q000000981:diagnostic imaging*; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D016253:Microscopy, Immunoelectron
chemical_list: D000418:Albumins
keywords: albumin; blood-brain barrier; diabetes mellitus; periventricular area