title: Next-Generation Sequencing in the Mycology Lab.
abstract: New state-of-the-art techniques in sequencing offer valuable tools in both detection of mycobiota and in understanding of the molecular mechanisms of resistance against antifungal compounds and virulence. Introduction of new sequencing platform with enhanced capacity and a reduction in costs for sequence analysis provides a potential powerful tool in mycological diagnosis and research. In this review, we summarize the applications of next-generation sequencing techniques in mycology.
authors.affiliation: Department of Medical Microbiology, Radboud University Medical Centre, Box 9101, 6500 HB Nijmegen, The Netherlands.
authors.forename: Jan
authors.initials: J
authors.lastname: Zoll
authors.affiliation: Institut Pasteur, Unité de Mycologie Moléculaire, Paris, France.
authors.forename: Eveline
authors.initials: E
authors.lastname: Snelders
authors.affiliation: Department of Medical Microbiology, Radboud University Medical Centre, Box 9101, 6500 HB Nijmegen, The Netherlands.
authors.forename: Paul E
authors.initials: PE
authors.lastname: Verweij
authors.affiliation: Department of Medical Microbiology, Radboud University Medical Centre, Box 9101, 6500 HB Nijmegen, The Netherlands.
authors.forename: Willem J G
authors.initials: WJ
authors.lastname: Melchers
keywords: Clinical mycology lab issues; Mycobiome; Next-generation sequencing; Resistance; Review; Whole genome sequencing