title: The Effects of Paleoclimatic Events on Mediterranean Trout: Preliminary Evidences from Ancient DNA.
abstract: In this pilot study for the first time, ancient DNA has been extracted from bone remains of Salmo trutta. These samples were from a stratigraphic succession located in a coastal cave of Calabria (southern Italy) inhabited by humans from upper Palaeolithic to historical times. Seven pairs of primers were used to PCR-amplify and sequence from 128 to 410 bp of the mtDNA control region of eleven samples. Three haplotypes were observed: two (ADcs-1 and MEcs-1) already described in rivers from the Italian peninsula; one (ATcs-33) belonging to the southern Atlantic clade of the AT Salmo trutta mtDNA lineage (sensu Bernatchez). The prehistoric occurrence of this latter haplotype in the water courses of the Italian peninsula has been detected for the first time in this study. Finally, we observed a correspondence between frequency of trout remains and variation in haplotype diversity that we related with ecological and demographic changes resulting from a period of rapid cooling known as the Younger Dryas.
authors.affiliation: Dipartimento di Scienze della Vita e dell'Ambiente (DiSVA), Università Politecnica delle Marche, Via Brecce Bianche, 60131 Ancona, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Splendiani
authors.affiliation: Dipartimento di Scienze della Vita e dell'Ambiente (DiSVA), Università Politecnica delle Marche, Via Brecce Bianche, 60131 Ancona, Italy.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Fioravanti
authors.affiliation: Dipartimento di Scienze della Vita e dell'Ambiente (DiSVA), Università Politecnica delle Marche, Via Brecce Bianche, 60131 Ancona, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Giovannotti
authors.affiliation: Dipartimento di Scienze della Vita e dell'Ambiente (DiSVA), Università Politecnica delle Marche, Via Brecce Bianche, 60131 Ancona, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Negri
authors.affiliation: Dipartimento di Scienze della Vita e dell'Ambiente (DiSVA), Università Politecnica delle Marche, Via Brecce Bianche, 60131 Ancona, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Ruggeri
authors.affiliation: Dipartimento di Scienze della Vita e dell'Ambiente (DiSVA), Università Politecnica delle Marche, Via Brecce Bianche, 60131 Ancona, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Olivieri
authors.affiliation: Dipartimento di Scienze della Vita e dell'Ambiente (DiSVA), Università Politecnica delle Marche, Via Brecce Bianche, 60131 Ancona, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Nisi Cerioni
authors.affiliation: Dipartimento di Chimica, Biologia e Biotecnologie, Università di Perugia, Via Elce di Sotto, 06123 Perugia, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Lorenzoni
authors.affiliation: Dipartimento di Scienze della Vita e dell'Ambiente (DiSVA), Università Politecnica delle Marche, Via Brecce Bianche, 60131 Ancona, Italy.|Istituto Italiano di Paleontologia Umana, Is.I.P.U., Convitto Nazionale Regina Margherita, Piazza Ruggero Bonghi 2. 03012 Anagni, Italy.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Caputo Barucchi
mesh_terms: D000818:Animals; D001483:Base Sequence; D001842:Bone and Bones / Q000033:anatomy & histology; D002138:Calibration; D002980:Climate; D000072441:DNA, Ancient / Q000032:analysis*; D005580:Fossils; D005843:Geography; D006116:Greenland; D006239:Haplotypes / Q000235:genetics; D007558:Italy; D019083:Mediterranean Region; D008928:Mitochondria / Q000235:genetics; D010163:Paleontology; D013997:Time Factors; D014337:Trout / Q000235:genetics*
chemical_list: D000072441:DNA, Ancient