title: Therapeutic Effects of CUR-Activated Human Umbilical Cord Mesenchymal Stem Cells on 1-Methyl-4-phenylpyridine-Induced Parkinson's Disease Cell Model.
abstract: The purpose of this study is to evaluate the therapeutic effects of human umbilical cord-derived mesenchymal stem cells (hUC-MSC) activated by curcumin (CUR) on PC12 cells induced by 1-methyl-4-phenylpyridinium ion (MPP+), a cell model of Parkinson's disease (PD). The supernatant of hUC-MSC and hUC-MSC activated by 5 µmol/L CUR (hUC-MSC-CUR) were collected in accordance with the same concentration. The cell proliferation and differentiation potential to dopaminergic neuronal cells and antioxidation were observed in PC12 cells after being treated with the above two supernatants and 5 µmol/L CUR. The results showed that the hUC-MSC-CUR could more obviously promote the proliferation and the expression of tyrosine hydroxylase (TH) and microtubule associated protein-2 (MAP2) and significantly decreased the expression of nitric oxide (NO) and inducible nitric oxide synthase (iNOS) in PC12 cells. Furtherly, cytokines detection gave a clue that the expression of IL-6, IL-10, and NGF was significantly higher in the group treated with the hUC-MSC-CUR compared to those of other two groups. Therefore, the hUC-MSC-CUR may be a potential strategy to promote the proliferation and differentiation of PD cell model, therefore providing new insights into a novel therapeutic approach in PD.
authors.affiliation: Neurology Department of General Hospital of Jinan Military Region, Jinan, Shandong 250031, China.
authors.forename: Li
authors.initials: L
authors.lastname: Jinfeng
authors.affiliation: The Neurology Department, The 148th Hospital, Zibo, Shandong 255300, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Yunliang
authors.affiliation: Sanbo Brain Hospital Capital Medical University, Haidian District, Beijing 100093, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Xinshan
authors.affiliation: Medical School of Henan University, Zhengzhou, Henan 475000, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Yutong
authors.affiliation: The Neurology Department, The 148th Hospital, Zibo, Shandong 255300, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Shanshan
authors.affiliation: Neurology Department, The Second Hospital Affiliated to Zhengzhou University, Zhengzhou, Henan 450014, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Peng
authors.affiliation: Neurology Department, The Second Hospital Affiliated to Zhengzhou University, Zhengzhou, Henan 450014, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xiaopeng
authors.affiliation: Neurology Department, The Second Hospital Affiliated to Zhengzhou University, Zhengzhou, Henan 450014, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhixiu
authors.affiliation: Neurology Department, The Second Hospital Affiliated to Zhengzhou University, Zhengzhou, Henan 450014, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Qingshan
authors.affiliation: The Neurology Department, The 148th Hospital, Zibo, Shandong 255300, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Honglei
authors.affiliation: Neurology Department of General Hospital of Jinan Military Region, Jinan, Shandong 250031, China.
authors.forename: Cao
authors.initials: C
authors.lastname: Xia
authors.affiliation: Department of Medicine, The University of Chicago, Chicago, IL 60637, USA.
authors.forename: Wang
authors.initials: W
authors.lastname: Hongwei
authors.affiliation: Neurology Department of General Hospital of Jinan Military Region, Jinan, Shandong 250031, China.
authors.forename: Cao
authors.initials: C
authors.lastname: Bingzhen
mesh_terms: D015655:1-Methyl-4-phenylpyridinium; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D020169:Caspases / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002469:Cell Separation; D003474:Curcumin / Q000494:pharmacology / Q000627:therapeutic use*; D016207:Cytokines / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism; D050483:Dopamine Plasma Membrane Transport Proteins / Q000378:metabolism; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D008954:Models, Biological; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D016716:PC12 Cells; D010300:Parkinson Disease / Q000188:drug therapy / Q000628:therapy*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism; D014470:Umbilical Cord / Q000166:cytology*
chemical_list: D016207:Cytokines; D050483:Dopamine Plasma Membrane Transport Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D014446:Tyrosine 3-Monooxygenase; D020169:Caspases; D003474:Curcumin; D015655:1-Methyl-4-phenylpyridinium; D004298:Dopamine