title: Evaluation of the pri-miR-34b/c rs4938723 polymorphism and its association with breast cancer risk.
abstract: MicroRNAs (miRNAs or miRs) are a family of small non-coding RNAs that function as oncogenes or tumor suppressor genes. Recent evidence suggests that the pri-miR-34b/c rs4938723 variant is associated with the development of cancer. At present, there is an inconsistent association between the single-nucleotide polymorphism in pri-miR-34b/c and cancer in the limited studies. The present study is a case-control investigation, with 263 breast cancer (BC) patients and 221 control women, which examined the potential association of the pri-miR-34b/c rs4938723 polymorphisms with BC susceptibility. The polymorphisms were genotyped by the polymerase chain reaction restriction fragment length polymorphism method. No significant association between the pri-miR-34b/c rs4938723 variant and BC was identified [TC vs. TT: Odds ratio (OR), 0.87; 95% confidence interval (CI), 0.60-1.26; P=0.506; CC vs. TT: OR, 1.22; 95% CI, 0.61-2.47; P=0.600; TC+CC vs. TT: OR, 0.91; 95% CI, 0.64-1.31; P=0.648; CC vs. TT+TC: OR, 1.32; 95% CI, 0.67-2.59; P=0.498; C vs. T: OR, 0.99; 95% CI, 0.75-1.31; P=0.986]. However, a significant association was observed between the pri-miR-34b/c rs4938723 genotypes and clinicopathological characteristics, such a grade, progesterone receptor and human epidermal growth factor receptor 2 status were observed (P<0.05). These findings suggest that the pri-miR-34b/c rs4938723 variant may not be a risk factor for the development of BC.
authors.affiliation: Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan 98167, Iran; Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan 98167, Iran.
authors.forename: Sara
authors.initials: S
authors.lastname: Sanaei
authors.affiliation: Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan 98167, Iran; Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan 98167, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Hashemi
authors.affiliation: Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan 98167, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Rezaei
authors.affiliation: Department of Internal Medicine, School of Medicine, Zahedan University of Medical Sciences, Zahedan 98167, Iran.
authors.forename: Seyed Mehdi
authors.initials: SM
authors.lastname: Hashemi
authors.affiliation: Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan 98167, Iran.
authors.forename: Gholamreza
authors.initials: G
authors.lastname: Bahari
authors.affiliation: Department of Human Anatomy and Cell Science, College of Medicine, Faculty of Health Sciences, University of Manitoba, Winnipeg, MB R3E 0J9, Canada.
authors.forename: Saeid
authors.initials: S
authors.lastname: Ghavami
keywords: breast cancer; polymorphism; pri-miR-34b/c