title: Transgene-free human induced pluripotent stem cell line (HS5-SV.hiPS) generated from cesarean scar-derived fibroblasts.
abstract: Transgene-free human HS5-SV.hiPS line was generated from human cesarean scar-derived fibroblasts using temperature-sensitive Sendai virus vectors carrying Oct4, Sox2, cMyc and Klf4 exogenous transcriptional factors. The viral constructs were eliminated from HS5-SV.hiPS line through heat treatment. Transgene-free HS5-SV.hiPS cells expressed pluripotent associated transcription factors Oct4, Nanog, Sox2, Rex1 and surface markers SSEA-4, TRA-1-60 and OCT4. HS5-SV.hiPS cells formed embryoid bodies and differentiated into three embryonic germ layers in vivo. HS5-SV.hiPS cells maintained their normal karyotype (46, XX) after culture for extended period. HS5-SV.hiPS displayed the similar pattern of DNA fingerprinting to the parenteral scar-derived fibroblasts.
authors.affiliation: Human Embryonic Stem Cell Research Center, Reproductive Medicine Unit, Department of Obstetrics and Gynecology, Chulalongkorn University, Bangkok, Thailand. Electronic address: ruttachuk.r@chula.ac.th.
authors.forename: Ruttachuk
authors.initials: R
authors.lastname: Rungsiwiwut
authors.affiliation: Human Embryonic Stem Cell Research Center, Reproductive Medicine Unit, Department of Obstetrics and Gynecology, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Wipawee
authors.initials: W
authors.lastname: Pavarajarn
authors.affiliation: Human Embryonic Stem Cell Research Center, Reproductive Medicine Unit, Department of Obstetrics and Gynecology, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Pranee
authors.initials: P
authors.lastname: Numchaisrika
authors.affiliation: Human Embryonic Stem Cell Research Center, Reproductive Medicine Unit, Department of Obstetrics and Gynecology, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Pramuan
authors.initials: P
authors.lastname: Virutamasen
authors.affiliation: Human Embryonic Stem Cell Research Center, Reproductive Medicine Unit, Department of Obstetrics and Gynecology, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Kamthorn
authors.initials: K
authors.lastname: Pruksananonda
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line / Q000166:cytology*; D002585:Cesarean Section; D002921:Cicatrix / Q000473:pathology*; D005260:Female; D005347:Fibroblasts / Q000473:pathology*; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D007621:Karyotyping; D000090062:Kruppel-Like Factor 4; D008819:Mice, Nude; D019076:Transgenes