title: Effects of GnRH on Neurite Outgrowth, Neurofilament and Spinophilin Proteins Expression in Cultured Spinal Cord Neurons of Rat Embryos.
abstract: It has been previously described the presence of GnRH receptor in spinal cord neurons of rat embryos and adult rats. However, the functional role of these receptors has not been studied. In this work, the effect of GnRH on neurite outgrowth and cytoskeletal protein expression in cultured spinal cord neurons of rat embryos was analyzed. Specifically, neurofilaments of 68 and 200 kDa by immunoblot assays and spinophilin mRNA expression by RT-PCR. Results show that GnRH stimulates neurite outgrowth in addition to an increase in neurofilaments and spinophilin expression. These findings suggest that GnRH may play a role as neuromodulator in neuronal plasticity and that could be considered as a potential factor for neuronal regeneration in spinal cord injuries.
authors.affiliation: Laboratory of Neurophysiology, Department of Physiology and Pharmacology, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Av. Universidad 940, Col. Ciudad Universitaria, Aguascalientes, AGS, C.P. 20131, Mexico. jlquinta@correo.uaa.mx.
authors.forename: J Luis
authors.initials: JL
authors.lastname: Quintanar
authors.affiliation: Laboratory of Neurophysiology, Department of Physiology and Pharmacology, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Av. Universidad 940, Col. Ciudad Universitaria, Aguascalientes, AGS, C.P. 20131, Mexico.
authors.forename: Denisse
authors.initials: D
authors.lastname: Calderón-Vallejo
authors.affiliation: Laboratory of Neurophysiology, Department of Physiology and Pharmacology, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Av. Universidad 940, Col. Ciudad Universitaria, Aguascalientes, AGS, C.P. 20131, Mexico.
authors.forename: Irma
authors.initials: I
authors.lastname: Hernández-Jasso
mesh_terms: D000818:Animals; D001369:Axons / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D007382:Intermediate Filaments / Q000187:drug effects*; D016501:Neurites / Q000187:drug effects*; D016900:Neurofilament Proteins / Q000378:metabolism; D000071446:Neuronal Outgrowth / Q000187:drug effects*; D017208:Rats, Wistar; D011966:Receptors, LHRH / Q000378:metabolism; D013116:Spinal Cord / Q000187:drug effects* / Q000196:embryology / Q000378:metabolism
chemical_list: D016900:Neurofilament Proteins; D011966:Receptors, LHRH; D007987:Gonadotropin-Releasing Hormone
keywords: GnRH-R; Gonadotropin-releasing hormone; NFs; Neurotrophic effect; Spinal cord neurons