title: Wide field-of-view, multi-region, two-photon imaging of neuronal activity in the mammalian brain.
abstract: Two-photon calcium imaging provides an optical readout of neuronal activity in populations of neurons with subcellular resolution. However, conventional two-photon imaging systems are limited in their field of view to ∼1 mm(2), precluding the visualization of multiple cortical areas simultaneously. Here, we demonstrate a two-photon microscope with an expanded field of view (>9.5 mm(2)) for rapidly reconfigurable simultaneous scanning of widely separated populations of neurons. We custom designed and assembled an optimized scan engine, objective, and two independently positionable, temporally multiplexed excitation pathways. We used this new microscope to measure activity correlations between two cortical visual areas in mice during visual processing.
authors.affiliation: Neuroscience Center, University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA.|Carolina Institute for Developmental Disabilities, University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA.
authors.forename: Jeffrey N
authors.initials: JN
authors.lastname: Stirman
authors.affiliation: Neuroscience Center, University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA.|Department of Pharmacology, University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA.
authors.forename: Ikuko T
authors.initials: IT
authors.lastname: Smith
authors.affiliation: Department of Electrical and Computer Engineering, North Carolina State University, Raleigh, North Carolina, USA.
authors.forename: Michael W
authors.initials: MW
authors.lastname: Kudenov
authors.affiliation: Neuroscience Center, University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA.|Carolina Institute for Developmental Disabilities, University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA.|Department of Cell Biology and Physiology, University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA.|Neurobiology Curriculum, University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA.
authors.forename: Spencer L
authors.initials: SL
authors.lastname: Smith
mesh_terms: D000818:Animals; D001931:Brain Mapping / Q000295:instrumentation* / Q000379:methods; D007089:Image Enhancement / Q000295:instrumentation / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation* / Q000379:methods; D009415:Nerve Net / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014793:Visual Cortex / Q000502:physiology*; D014796:Visual Perception / Q000502:physiology*; D056969:Voltage-Sensitive Dye Imaging / Q000295:instrumentation* / Q000379:methods