title: Novel imatinib-loaded silver nanoparticles for enhanced apoptosis of human breast cancer MCF-7 cells.
abstract: In the current study, in vitro biological feature of imatinib-loaded silver nanoparticles (IMAB-AgNPs) on human breast cancer cell line was investigated. The formation of synthesized silver nanoparticles (AgNPs) was characterized by UV-Visible spectroscopy, EDS, TEM imaging, SEM, FTIR, DLS and Zeta potentiometer. The developed IMAB-AgNPs with maximum percentage of loading efficiency was demonstrated in the average of 130 nm and mostly spherical. Additionally, in vitro drug release study showed a slow and continuous release of imatinib over a period of 80 h. We demonstrated that the synthesized IMAB-AgNPs exhibited a dose-dependent cytotoxicity against MCF-7 cell line. Then, real-time PCR method was also applied for the investigation of Bax and Bcl-2 gene expression in the cells. Comparing IMAB-AgNPs to AgNPs and Imatinib revealed the ability of IMAB-AgNPs to up-regulating Bax/Bcl-2 ratio. An induction of apoptosis was evidenced by Annexin-V/PI detection assay. Based on the current obtained data, the IMAB-AgNPs can exhibit inhibitory effect on viability through up regulation of apoptosis in MCF-7 cancer cells, which provides influencing evidence for the green synthesized AgNPs as a promising sustained drug delivery system.
authors.affiliation: a Department of Biology, Science and Research Branch , Islamic Azad University , Tehran , Iran.
authors.forename: Seyed Ataollah
authors.initials: SA
authors.lastname: Sadat Shandiz
authors.affiliation: b Department of Radiopharmacy, Faculty of Pharmacy , Tehran University of Medical Sciences, Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Shafiee Ardestani
authors.affiliation: c Biotechnology Research Center, Venom & Biotherapeutics Molecules Laboratory, Pasteur Institute of Iran , Tehran , Iran.
authors.forename: Delavar
authors.initials: D
authors.lastname: Shahbazzadeh
authors.affiliation: b Department of Radiopharmacy, Faculty of Pharmacy , Tehran University of Medical Sciences, Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Artin
authors.initials: A
authors.lastname: Assadi
authors.affiliation: d Department of Pilot Nanobiotechnology , New Technologies Research Group, Pasteur Institute of Iran , Tehran , Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Ahangari Cohan
authors.affiliation: e Department of Immunology , School of Medicine, Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Vahid
authors.initials: V
authors.lastname: Asgary
authors.affiliation: f Department of Phytochemistry and Essential Oils Technology, Faculty of Pharmaceutical Chemistry , Pharmaceutical Sciences Branch, Islamic Azad University , Tehran , Iran.
authors.forename: Soheil
authors.initials: S
authors.lastname: Salehi
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D016503:Drug Delivery Systems / Q000379:methods*; D005260:Female; D006801:Humans; D000068877:Imatinib Mesylate / Q000737:chemistry / Q000494:pharmacology; D061986:MCF-7 Cells; D053768:Metal Nanoparticles / Q000737:chemistry / Q000627:therapeutic use; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D012834:Silver / Q000737:chemistry / Q000494:pharmacology; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: C494826:BAX protein, human; C000595707:BCL2 protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D012834:Silver; D000068877:Imatinib Mesylate
keywords: Apoptosis; MCF-7; imatinib; silver nanoparticles