title: Generation of acid mine drainage around the Karaerik copper mine (Espiye, Giresun, NE Turkey): implications from the bacterial population in the Acısu effluent.
abstract: The Karaerik Cu mine is a worked-out deposit with large volumes of tailings and slags which were left around the mine site without any protection. Natural feeding of these material and run-off water from the mineralised zones into the Acısu effluent causes a serious environmental degradation and creation of acid mine drainage (AMD) along its entire length. This research aims at modelling the formation of AMD with a specific attempt on the characterisation of the bacterial population in association with AMD and their role on its occurrence. Based on 16SrRNA analyses of the clones obtained from a composite water sample, the bacterial community was determined to consist of Acidithiobacillus ferrivorans, Ferrovum myxofaciens, Leptospirillum ferrooxidans and Acidithiobacillus ferrooxidans as iron-oxidising bacteria, Acidocella facilis, Acidocella aluminiidurans, Acidiphilium cryptum and Acidiphilium multivorum as iron-reducing bacteria, and Acidithiobacillus ferrivorans, Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans and Acidiphilium cryptum as sulphur-oxidising bacteria. This association of bacteria with varying roles was interpreted as evidence of a concomitant occurrence of sulphur and iron cycles during the generation of AMD along the Acısu effluent draining the Karaerik mine.
authors.affiliation: Department of Geology, Karadeniz Technical University, 61080, Trabzon, Turkey. ssaglam@ktu.edu.tr.
authors.forename: Emine Selva
authors.initials: ES
authors.lastname: Sağlam
authors.affiliation: Department of Geology, Karadeniz Technical University, 61080, Trabzon, Turkey.
authors.forename: Miğraç
authors.initials: M
authors.lastname: Akçay
authors.affiliation: Bulancak Kadir Karabaş School of Applied Sciences, Giresun University, Giresun, Turkey.
authors.forename: Dilşat Nigar
authors.initials: DN
authors.lastname: Çolak
authors.affiliation: Department of Molecular Biology and Genetic, Karadeniz Technical University, 61080, Trabzon, Turkey.
authors.forename: Kadriye
authors.initials: K
authors.lastname: İnan Bektaş
authors.affiliation: Department of Biology, Karadeniz Technical University, 61080, Trabzon, Turkey.
authors.forename: Ali Osman
authors.initials: AO
authors.lastname: Beldüz
mesh_terms: D041801:Acidiphilium / Q000145:classification / Q000302:isolation & purification; D042763:Acidithiobacillus / Q000145:classification / Q000302:isolation & purification; D000143:Acids / Q000032:analysis; D019015:Geologic Sediments / Q000737:chemistry / Q000382:microbiology*; D060587:Groundwater / Q000737:chemistry / Q000382:microbiology*; D007501:Iron / Q000378:metabolism; D016953:Leptospiraceae / Q000145:classification / Q000302:isolation & purification; D064307:Microbiota; D008906:Mining; D010084:Oxidation-Reduction; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013455:Sulfur / Q000378:metabolism
chemical_list: D000143:Acids; D012336:RNA, Ribosomal, 16S; D013455:Sulfur; D007501:Iron
keywords: 16S rRNA; Acid mine drainage; Acidiphilium cryptum; Acidithiobacillus ferrivorans; Acidocella facilis; Ferrovum myxofaciens; Leptospirillum ferrooxidans