title: Quantitative Analysis of Caspase-1 Activity in Living Cells Through Dynamic Equilibrium of Chlorophyll-Based Nano-assembly Modulated Photoacoustic Signals.
abstract: In situ construction of self-assemblies with unique property in living systems is a promising direction in the biomedical field. The noninvasive methods for significant enzyme activity in living cells or living subjects are imperative and meantime challenge tasks. The dynamic process of self-assembly of chlorophyll-based molecules in complex biological systems can be monitored by photoacoustic signals, which supports a noninvasive way to understand and quantitatively measure the activity of caspase-1. Furthermore, the activity of caspase-1 enables reflection of the bacterial infection in the early stage. Here, we present a biocompatible self-assembly from chlorophyll-peptide derivatives and first correlate the dynamic equilibrium with ratiometric photoacoustic signals. The intracellular equilibrium was managed by a bacterial infection precaution protein, i.e., caspase-1. This system offers a trial of noninvasive method to quantitative detection and real-time monitoring of bacterial infection in the early stage.
authors.affiliation: CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST) Department Institution , No. 11 Beiyitiao, Beijing, China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Li
authors.affiliation: CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST) Department Institution , No. 11 Beiyitiao, Beijing, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Zeng
authors.affiliation: CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST) Department Institution , No. 11 Beiyitiao, Beijing, China.|State Key Laboratory of Hollow Fiber Membrane Materials and Processes, School of Environmental and Chemical Engineering, Tianjin Polytechnic University , Tianin, China.
authors.forename: Wei-Jiao
authors.initials: WJ
authors.lastname: Liu
authors.affiliation: CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST) Department Institution , No. 11 Beiyitiao, Beijing, China.|Lab of Low-Dimensional Materials Chemistry, Key Laboratory for Ultrafine Materials of Ministry of Education School of Materials Science and Engineering, East China University of Science and Technology , Shanghai, China.
authors.forename: Xue-Feng
authors.initials: XF
authors.lastname: Hu
authors.affiliation: Lab of Low-Dimensional Materials Chemistry, Key Laboratory for Ultrafine Materials of Ministry of Education School of Materials Science and Engineering, East China University of Science and Technology , Shanghai, China.
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Hollow Fiber Membrane Materials and Processes, School of Environmental and Chemical Engineering, Tianjin Polytechnic University , Tianin, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Pan
authors.affiliation: State Key Laboratory of Hollow Fiber Membrane Materials and Processes, School of Environmental and Chemical Engineering, Tianjin Polytechnic University , Tianin, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Wan
authors.affiliation: CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology (NCNST) Department Institution , No. 11 Beiyitiao, Beijing, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D020170:Caspase 1 / Q000032:analysis / Q000378:metabolism*; D002734:Chlorophyll / Q000737:chemistry*; D008264:Macrophages / Q000201:enzymology*; D051379:Mice; D049329:Nanostructures / Q000737:chemistry*; D061088:Photoacoustic Techniques / Q000379:methods*; D000067996:RAW 264.7 Cells; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013203:Staphylococcal Infections / Q000201:enzymology; D013211:Staphylococcus aureus
chemical_list: D002734:Chlorophyll; C000628184:Casp1 protein, mouse; D020170:Caspase 1
keywords: bacterial infection; caspase-1; chlorophyll; dynamic equilibrium; self-assembly