title: Disintegrin targeting of an αvβ3 integrin-over-expressing high-metastatic human osteosarcoma with echistatin inhibits cell proliferation, migration, invasion and adhesion in vitro.
abstract: The in vitro efficacy of the disintegrin echistatin was tested on a high-metastatic variant of 143B human osteosarcoma, 143B-LM4, which over-expresses αvβ3 integrin. Echistatin is an RGD cyclic peptide and an antagonist of αvβ3 integrin. In the present study, echistatin inhibited cell proliferation, migration, invasion, and adhesion of 143B-LM4 cells. 143B-LM4 cell proliferation decreased after treatment with echistatin in a time-dependent and dose-dependent manner (P <0.01). In vitro migration and invasion of 143B-LM4 cells were also inhibited by echistatin in a dose-dependent manner (P <0.01, respectively). Cell adhesion to vitronectin of 143B-LM4 cells was also inhibited by echistatin in a dose-dependent manner (P <0.01). These results suggest that αvβ3 integrin may be an effective target for osteosarcoma.
authors.affiliation: AntiCancer, Inc., San Diego, CA 92111, USA.|Department of Surgery, University of California, San Diego, CA 92103, USA.|Department of Orthopedic Surgery, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan 903-0125.
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Tome
authors.affiliation: AntiCancer, Inc., San Diego, CA 92111, USA.|Department of Orthopaedic Surgery, Graduate School of Medical Science, Kanazawa University, Kanazawa, Japan 920-8641.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kimura
authors.affiliation: AntiCancer, Inc., San Diego, CA 92111, USA.|Department of Surgery, University of California, San Diego, CA 92103, USA.|Department of Orthopedic Surgery, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan 903-0125.
authors.forename: Tasuku
authors.initials: T
authors.lastname: Kiyuna
authors.affiliation: Department of Physiology, Graduate School of Medical Science, Kanazawa University, Kanazawa, Japan 920-8641.
authors.forename: Naotoshi
authors.initials: N
authors.lastname: Sugimoto
authors.affiliation: Department of Orthopaedic Surgery, Graduate School of Medical Science, Kanazawa University, Kanazawa, Japan 920-8641.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tsuchiya
authors.affiliation: Department of Orthopedic Surgery, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan 903-0125.
authors.forename: Fuminori
authors.initials: F
authors.lastname: Kanaya
authors.affiliation: Department of Surgery, University of California, San Diego, CA 92103, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Bouvet
authors.affiliation: AntiCancer, Inc., San Diego, CA 92111, USA.|Department of Surgery, University of California, San Diego, CA 92103, USA.
authors.forename: Robert M
authors.initials: RM
authors.lastname: Hoffman
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D001859:Bone Neoplasms / Q000473:pathology*; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D019483:Disintegrins / Q000494:pharmacology; D006801:Humans; D039302:Integrin alphaVbeta3 / Q000037:antagonists & inhibitors*; D036341:Intercellular Signaling Peptides and Proteins; D009361:Neoplasm Invasiveness / Q000473:pathology; D012516:Osteosarcoma / Q000473:pathology*; D010455:Peptides / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D019483:Disintegrins; D039302:Integrin alphaVbeta3; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; C057986:echistatin
keywords: echistatin; green fluorescent protein/red fluorescent protein; metastasis; osteosarcoma; αv β3 integrin