title: Synthesis, Characterization and Application of Thermoresponsive Polyhydroxyalkanoate-graft-Poly(N-isopropylacrylamide).
abstract: A thermoresponsive graft copolymer polyhydroxyalkanoate-g-poly(N-isopropylacrylamide) or short as PHA-g-PNIPAm, was successfully synthesized via a three-step reaction. First, PNIPAm oligomer with a trithiocarbonate-based chain transfer agent (CTA), short as PNIPAm-CTA, with designed polymerization degree was synthesized via reversible addition-fragmentation chain transfer (RAFT) polymerization. Subsequently, the PNIPAm-CTA was treated with n-butylamine for aminolysis in order to obtain a pendant thiol group at the end of the chain (PNIPAm-SH). Finally, the PNIPAm-SH was grafted onto unsaturated P(3HDD-co-3H10U), a random copolymer of 3-hydroxydodecanoate (3HDD) and 3-hydroxy-10-undecylenate (3H10U), via a thiol-ene click reaction. Enhanced hydrophilicity and thermoresponsive property of the resulted PHA-g-PNIPAm were confirmed by water contact angle studies. The biocompatibility of PHA-g-PNIPAm was comparable to poly-3-hydroxybutyrate (PHB). The graft copolymer PHA-g-PNIPAm based on biopolyester PHA could be a promising material for biomedical applications.
authors.affiliation: Center of Synthetic and Systems Biology, School of Life Science, Tsinghua-Peking Center for Life Sciences, Tsinghua University , Beijing 100084, China.|Center for Nano and Micro Mechanics, Tsinghua University , Beijing 100084, China.
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Ma
authors.affiliation: Center of Synthetic and Systems Biology, School of Life Science, Tsinghua-Peking Center for Life Sciences, Tsinghua University , Beijing 100084, China.
authors.forename: Dai-Xu
authors.initials: DX
authors.lastname: Wei
authors.affiliation: Center of Synthetic and Systems Biology, School of Life Science, Tsinghua-Peking Center for Life Sciences, Tsinghua University , Beijing 100084, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yao
authors.affiliation: Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen , Copenhagen 2100, Denmark.
authors.forename: Lin-Ping
authors.initials: LP
authors.lastname: Wu
authors.affiliation: Center of Synthetic and Systems Biology, School of Life Science, Tsinghua-Peking Center for Life Sciences, Tsinghua University , Beijing 100084, China.|Center for Nano and Micro Mechanics, Tsinghua University , Beijing 100084, China.
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Chen
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D008422:Materials Testing; D008956:Models, Chemical; D008968:Molecular Conformation; D054813:Polyhydroxyalkanoates / Q000737:chemistry*; D058105:Polymerization; D011108:Polymers / Q000737:chemistry*; D013696:Temperature; D013816:Thermodynamics; D014867:Water / Q000737:chemistry*
chemical_list: D000180:Acrylic Resins; D054813:Polyhydroxyalkanoates; D011108:Polymers; D014867:Water; C052970:poly-N-isopropylacrylamide