title: MiR-330-5p regulates tyrosinase and PDIA3 expression and suppresses cell proliferation and invasion in cutaneous malignant melanoma.
abstract: Increasing evidence has suggested that miR-330-5p can function as a tumor suppressor in different types of cancers. However, the effects and underlying mechanisms of miR-330-5p in the development of cutaneous malignant melanoma (CMM) remain largely unknown. The aim of the present study was to investigate the role of miR-330-5p in CMM and to determine the molecular mechanisms underlying its action.
  
  
  
  The expression level of miR-330-5p was detected in 26 cases of primary CMM tissues and cell lines by real-time quantitative polymerase chain reaction. We also assessed whether overexpression of miR-330-5p influences in vitro cell proliferation, invasion, and migration. Western blotting analysis was used to detect the influence of miR-330-5p on the targets, and Pearson analysis was used to calculate the correlation between the expression of targets gene and miR-330-5p in CMM tissues.
  
  
  
  Our study showed that miR-330-5p was downregulated in CMM tissues (P = 0.010) and cell lines (P < 0.05), and patients with high mitotic activity showed lower miR-330-5p expression levels (P = 0.002). Enforced expression of miR-330-5p inhibits malignant CMM cells proliferation and migration and led to downregulation of the TYR and PDIA3 protein. Moreover, the expression level of miR-330-5p in CMM tissues showed inverse relationship with the expression level of TYR and PDIA3 protein.
  
  
  
  In conclusion, our findings suggested that miR-330-5p represents a potential tumor-suppressive miRNA and plays an important role in CMM progression by suppressing TYR and PDIA3 expression.
authors.affiliation: Department of Dermatology, Xinxiang Central Hospital, Xinxiang, Henan, People's Republic of China. Electronic address: zhousw2014@163.com.
authors.forename: Bei-Bei
authors.initials: BB
authors.lastname: Su
authors.affiliation: Department of Head, Neck, and Breast Surgery, Xinxiang Central Hospital, Xinxiang, Henan, People's Republic of China.
authors.forename: Shu-Wei
authors.initials: SW
authors.lastname: Zhou
authors.affiliation: Department of Dermatology, Xinxiang Central Hospital, Xinxiang, Henan, People's Republic of China.
authors.forename: Cai-Bin
authors.initials: CB
authors.lastname: Gan
authors.affiliation: Department of Dermatology, Xinxiang Central Hospital, Xinxiang, Henan, People's Republic of China.
authors.forename: Xiao-Ning
authors.initials: XN
authors.lastname: Zhang
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D002460:Cell Line; D002465:Cell Movement; D049109:Cell Proliferation / Q000235:genetics; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008545:Melanoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D035683:MicroRNAs / Q000378:metabolism*; D014442:Monophenol Monooxygenase / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D019704:Protein Disulfide-Isomerases / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D012878:Skin Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D015854:Up-Regulation; D000096142:Cutaneous Malignant Melanoma
chemical_list: D014408:Biomarkers, Tumor; C570805:MIRN330 microRNA, human; D035683:MicroRNAs; D014442:Monophenol Monooxygenase; D019704:Protein Disulfide-Isomerases; C085868:PDIA3 protein, human
keywords: Cutaneous malignant melanoma; Function; PDIA3; TYR; miR-330-5p