title: MiR-367 negatively regulates apoptosis induced by adriamycin in osteosarcoma cells by targeting KLF4.
abstract: Diverse functions of microRNAs have been investigated in tumorigenesis in osteosarcoma (OS), involving the regulation of proliferation, invasion, migration, apoptosis and drug resistance. MiR-367 was found to be an oncogene and increased in OS. However, the function of miR-367 in drug resistance in OS cells is still unknown. In this study, we found that miR-367 was up-regulated in OS tissues and OS cell cultures. Meanwhile, treatment with adriamycin (ADR) induced apoptosis of OS cells with upregulation of miR-367. Notably, KLF4 was demonstrated to be a direct target of miR-367 by gene reporter assay, and miR-367 significantly blocked both mRNA and protein level of KLF4. In addition, overexpression of miR-367 markedly suppressed the increase of KLF4 induced by ADR in OS cells, as well as Bax and cleaved caspase-3, which were significantly reversed by anti-miR-367 transfection. Taken together, our data demonstrates that miR-367 and KLF4 play important roles in OS treatment and ADR resistance, suggesting that miR-367 is a potential biomarker of chemotherapy resistance in OS and also probably a novel therapeutic target against OS.
authors.affiliation: Department of Orthopedics, Changhai Hospital, The Second Military Medical University, 168 Changhai Road, Shanghai 200433, China.
authors.forename: Guang-Chao
authors.initials: GC
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, Changhai Hospital, The Second Military Medical University, 168 Changhai Road, Shanghai 200433, China.
authors.forename: Qian-Yun
authors.initials: QY
authors.lastname: He
authors.affiliation: Department of Orthopedics, Changhai Hospital, The Second Military Medical University, 168 Changhai Road, Shanghai 200433, China.
authors.forename: Da-Ke
authors.initials: DK
authors.lastname: Tong
authors.affiliation: Department of Orthopedics, Changhai Hospital, The Second Military Medical University, 168 Changhai Road, Shanghai 200433, China.
authors.forename: Chuan-Feng
authors.initials: CF
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, Changhai Hospital, The Second Military Medical University, 168 Changhai Road, Shanghai 200433, China.
authors.forename: Kang
authors.initials: K
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, Changhai Hospital, The Second Military Medical University, 168 Changhai Road, Shanghai 200433, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Ding
authors.affiliation: Department of Orthopedics, Changhai Hospital, The Second Military Medical University, 168 Changhai Road, Shanghai 200433, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Ji
authors.affiliation: Department of Orthopedics, Changhai Hospital, The Second Military Medical University, 168 Changhai Road, Shanghai 200433, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
keywords: Adriamycin; Apoptosis; Chemotherapy resistance; KLF4; MiR-367; Osteosarcoma