title: Micro RNA-17-92 cluster mediates interleukin-4-suppressed IL-10 expression in B cells.
abstract: The pathogenesis of allergen-related inflammation in the intestine is to be further understood. Micro RNA (miR) can regulate immune responses. This study aims to investigate the role of miR-17-92 cluster in the induction of food allergen-related inflammation in the intestine. In this study, a mouse model of food allergen-related intestinal inflammation was developed. Expression of miR-17-92 cluster in B cells of the intestinal mucosa was analyzed by real time quantitative RT-PCR. The results showed that the levels of miR-19a, one of the members of the miR-17-92 cluster, were detected in the B cells of the intestine of mice sensitized to ovalbumin, which was significantly higher than that in naïve control mice. The expression of IL-10 by B cells was significantly lower in the sensitized mice as compared with naive control mice. Exposure to IL-4 in the culture increased the expression of miR-19a as well as suppression the expression of IL-10 in B cells via remolding DNA structure at the IL-10 promoter locus. We conclude that B cells from sensitized mice show higher levels of miR-19a, which plays an important role in the suppression of IL-10 in the B cells.
authors.affiliation: ENT Institute of Shenzhen University School of MedicineShenzhen 518060, China; Longgang ENT HospitalShenzhen 518116, China; Brain Body Institute, McMaster UniversityHamilton, ON, Canada L8N 4A6.
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Liu
authors.affiliation: ENT Institute of Shenzhen University School of MedicineShenzhen 518060, China; Longgang ENT HospitalShenzhen 518116, China; Brain Body Institute, McMaster UniversityHamilton, ON, Canada L8N 4A6.
authors.forename: Gui
authors.initials: G
authors.lastname: Yang
authors.affiliation: ENT Institute of Shenzhen University School of MedicineShenzhen 518060, China; Longgang ENT HospitalShenzhen 518116, China; Brain Body Institute, McMaster UniversityHamilton, ON, Canada L8N 4A6.
authors.forename: Xiao-Rui
authors.initials: XR
authors.lastname: Geng
authors.affiliation: ENT Institute of Shenzhen University School of MedicineShenzhen 518060, China; Longgang ENT HospitalShenzhen 518116, China; Brain Body Institute, McMaster UniversityHamilton, ON, Canada L8N 4A6.
authors.forename: Jiang-Qi
authors.initials: JQ
authors.lastname: Liu
authors.affiliation: ENT Institute of Shenzhen University School of MedicineShenzhen 518060, China; Longgang ENT HospitalShenzhen 518116, China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Mo
authors.affiliation: ENT Institute of Shenzhen University School of Medicine Shenzhen 518060, China.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Liu
authors.affiliation: ENT Institute of Shenzhen University School of Medicine Shenzhen 518060, China.
authors.forename: Ping-Chang
authors.initials: PC
authors.lastname: Yang
keywords: Allergy; B cell; interleukin-10; intestine; micro RNA