title: Temporal and Spatial Expression of LGR5 After Acute Spinal Cord Injury in Adult Rats.
abstract: Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5), a well-characterized marker of stem cell and cancer stem cells (CSCs), is best known as a transmembrance receptor for increases canonical Wnt signaling amplitude. In addition, in some types of human cancers, LGR5 has been found to be overexpressed. However, the distribution and function of LGR5 in spinal cord injury (SCI) are still unknown. In this study, we examined LGR5 expression and cellular localization in rats following acute SCI. Western blot analysis and immunohistochemistry exhibited an important upregulation of LGR5 in injury spinal cord. Double immunofluorescence staining showed that LGR5 was co-expressed with glial fibrillary acidic protein (GFAP). Moreover, we detected that PCNA had colocalization with LGR5 and GFAP after SCI. In the vitro model, we could find the enhanced expression of LGR5 in the primary astrocyte which was induced by the lipopolysaccharide (LPS). In particular, we found the significantly decreased ability for proliferation when this LGR5-specific siRNA transfected primary astrocytes. In a word, this is the first description of LGR5 expression in spinal cord injury. These data indicated that LGR5 might be of great significance in CNS pathophysiology after SCI.
authors.affiliation: Department of Orthopedics, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Orthopedics, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.|Medical College, Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Hao
authors.affiliation: School of Nursing, Nantong University, Nantong, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Fu
authors.affiliation: Department of Orthopedics, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.|Medical College, Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.|Medical College, Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Mingchen
authors.initials: M
authors.lastname: Yu
authors.affiliation: The Second Affiliated Hospital, Nantong University, Nantong, 226001, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: He
authors.affiliation: Department of Orthopedics, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.|Medical College, Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Qian
authors.affiliation: Department of Orthopedics, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China. zhangfengdoctor@sina.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects; D004195:Disease Models, Animal; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D013119:Spinal Cord Injuries / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D005904:Glial Fibrillary Acidic Protein; C576179:Lgr5 protein, rat; D018809:Proliferating Cell Nuclear Antigen; D043562:Receptors, G-Protein-Coupled
keywords: Astrocyte; LGR5; Proliferation; Spinal cord injury