title: Ozonolysis combined with ultrasound as a pretreatment of sugarcane bagasse: Effect on the enzymatic saccharification and the physical and chemical characteristics of the substrate.
abstract: Sugarcane bagasse (SCB) was treated in three stages using ozone oxidation (O), washing in an alkaline medium (B) and ultrasonic irradiation (U). The impact of each pretreatment stage on the physical structure of the SCB was evaluated by its chemical composition, using an infrared technique (FTIR-ATR), and using thermogravimetric analysis (TGA/DTG). The pretreatment sequence O, B, U provided a significant reduction of lignin and hemicellulose, which was confirmed by changes in the absorption bands corresponding to these compounds, when observed using infrared. Thermogravimetric analysis confirmed an increased thermal stability in the treated sample due to the removal of hemicellulose and extractives during the pretreatment. This pretreatment released 391mg glucose/g from treated SCB after the enzymatic hydrolysis, corresponding to a yield of 94% of the cellulose available.
authors.affiliation: Department of Chemistry and Environmental Science, UNESP - Univ. Estadual Paulista - IBILCE, Rua Cristovao Colombo, 2265, Sao Jose do Rio Preto, São Paulo, Brazil.
authors.forename: Olavo Micali
authors.initials: OM
authors.lastname: Perrone
authors.affiliation: Department of Chemistry and Environmental Science, UNESP - Univ. Estadual Paulista - IBILCE, Rua Cristovao Colombo, 2265, Sao Jose do Rio Preto, São Paulo, Brazil.
authors.forename: Felippe Mariano
authors.initials: FM
authors.lastname: Colombari
authors.affiliation: Department of Chemistry and Environmental Science, UNESP - Univ. Estadual Paulista - IBILCE, Rua Cristovao Colombo, 2265, Sao Jose do Rio Preto, São Paulo, Brazil.
authors.forename: Jessika Souza
authors.initials: JS
authors.lastname: Rossi
authors.affiliation: Department of Chemistry and Environmental Science, UNESP - Univ. Estadual Paulista - IBILCE, Rua Cristovao Colombo, 2265, Sao Jose do Rio Preto, São Paulo, Brazil.
authors.forename: Marcia Maria Souza
authors.initials: MM
authors.lastname: Moretti
authors.affiliation: Department of Chemistry and Environmental Science, UNESP - Univ. Estadual Paulista - IBILCE, Rua Cristovao Colombo, 2265, Sao Jose do Rio Preto, São Paulo, Brazil.
authors.forename: Sidnei Emilio
authors.initials: SE
authors.lastname: Bordignon
authors.affiliation: Department of Chemistry and Environmental Science, UNESP - Univ. Estadual Paulista - IBILCE, Rua Cristovao Colombo, 2265, Sao Jose do Rio Preto, São Paulo, Brazil.
authors.forename: Christiane da Costa Carreira
authors.initials: Cda C
authors.lastname: Nunes
authors.affiliation: Department of Chemistry and Environmental Science, UNESP - Univ. Estadual Paulista - IBILCE, Rua Cristovao Colombo, 2265, Sao Jose do Rio Preto, São Paulo, Brazil.
authors.forename: Eleni
authors.initials: E
authors.lastname: Gomes
authors.affiliation: Department of Chemistry and Environmental Science, UNESP - Univ. Estadual Paulista - IBILCE, Rua Cristovao Colombo, 2265, Sao Jose do Rio Preto, São Paulo, Brazil.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Boscolo
authors.affiliation: Department of Chemistry and Environmental Science, UNESP - Univ. Estadual Paulista - IBILCE, Rua Cristovao Colombo, 2265, Sao Jose do Rio Preto, São Paulo, Brazil. Electronic address: dasilva@ibilce.unesp.br.
authors.forename: Roberto
authors.initials: R
authors.lastname: Da-Silva
mesh_terms: D002480:Cellulase / Q000737:chemistry / Q000378:metabolism*; D002482:Cellulose / Q000528:radiation effects; D005947:Glucose / Q000378:metabolism; D019220:High-Energy Shock Waves; D006868:Hydrolysis / Q000187:drug effects / Q000528:radiation effects; D008031:Lignin / Q000032:analysis / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D010126:Ozone / Q000032:analysis / Q000494:pharmacology*; D031786:Saccharum / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D013010:Sonication / Q000379:methods; D014465:Ultrasonics
chemical_list: D010126:Ozone; D002482:Cellulose; D008031:Lignin; C027433:bagasse; D002480:Cellulase; D005947:Glucose
keywords: Enzyme hydrolysis; Ozonolysis; Sugarcane pretreatment; Ultrasound