title: Kif1B Interacts with KBP to Promote Axon Elongation by Localizing a Microtubule Regulator to Growth Cones.
abstract: Delivery of proteins and organelles to the growth cone during axon extension relies on anterograde transport by kinesin motors. Though critical for neural circuit development, the mechanisms of cargo-specific anterograde transport during axon extension are only starting to be explored. Cargos of particular importance for axon outgrowth are microtubule modifiers, such as SCG10 (Stathmin-2). SCG10 is expressed solely during axon extension, localized to growth cones, and essential for axon outgrowth; however, the mechanisms of SCG10 transport and activity were still debated. Using zebrafish mutants and in vivo imaging, we identified the Kif1B motor and its interactor Kif1 binding protein (KBP) as critical for SCG10 transport to axon growth cones and complete axon extension. Axon truncation in kbp(st23) mutants can be suppressed by SCG10 overexpression, confirming the direct relationship between decreased SCG10 levels and failed axon outgrowth. Live imaging revealed that the reduced levels of SCG10 in kbp(st23) mutant growth cones led to altered microtubule stability, defining the mechanistic basis of axon truncation. Thus, our data reveal a novel role for the Kif1B-KBP complex in the anterograde transport of SCG10, which is necessary for proper microtubule dynamics and subsequent axon extension.
  
  
  
  Together, our data define the mechanistic underpinnings of failed axon outgrowth with loss of KBP or its associated motor, Kif1B. In addition, we provide conclusive evidence that this defect results from disruption of anterograde transport of SCG10. This is one of the first examples of a motor to be implicated in the essential transport of a discreet cargo necessary for axon extension. In addition, counter to previous in vitro and cell culture results, neither loss of the Kif1B motor nor KBP resulted in inhibition of mitochondrial transport. Altogether, our work links transport of SCG10 to the regulation of microtubule dynamics in the axon growth cone and enhances our understanding of this process during axon outgrowth.
authors.affiliation: Department of Cell, Developmental, & Cancer Biology, Oregon Health & Science University, Portland, Oregon 97239 nechipor@ohsu.edu drerup@ohsu.edu.
authors.forename: Catherine M
authors.initials: CM
authors.lastname: Drerup
authors.affiliation: Department of Cell, Developmental, & Cancer Biology, Oregon Health & Science University, Portland, Oregon 97239.
authors.forename: Sarah
authors.identifier: 0000-0002-0602-4391
authors.initials: S
authors.lastname: Lusk
authors.affiliation: Department of Cell, Developmental, & Cancer Biology, Oregon Health & Science University, Portland, Oregon 97239 nechipor@ohsu.edu drerup@ohsu.edu.
authors.forename: Alex
authors.initials: A
authors.lastname: Nechiporuk
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001369:Axons / Q000502:physiology*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D004625:Embryo, Nonmammalian; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D020439:Growth Cones / Q000378:metabolism*; D016547:Kinesins / Q000235:genetics / Q000378:metabolism*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism*; D021381:Protein Transport / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D050777:Stathmin / Q000235:genetics / Q000378:metabolism; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D008164:Luminescent Proteins; D012333:RNA, Messenger; C541121:SCG10 protein, zebrafish; D050777:Stathmin; D029961:Zebrafish Proteins; C527765:kif1bp protein, zebrafish; C542096:KIF1B protein, zebrafish; D016547:Kinesins
keywords: KBP; KIF1B; SCG10; axon extension; stathmin