title: Krüpple-like factors 7 and 6a mRNA expression in adult zebrafish central nervous system.
abstract: Krüpple-like factors (KLFs) are transcription factors with zinc finger DNA binding domains known to play important roles in brain development and central nervous system (CNS) regeneration. There is little information on KLFs expression in adult vertebrate CNS. In this study, we used in situ hybridization to examine Klf7 mRNA (klf7) and Klf6a mRNA (klf6a) expression in adult zebrafish CNS. Both klfs exhibit wide and similar expression in the zebrafish CNS. Brain areas containing strongly labeled cells include the ventricular regions of the dorsomedial telencephalon, the ventromedial telencephalon, periventricular regions of the thalamus and hypothalamus, torus longitudinalis, stratum periventriculare of the optic tectum, granular regions of the cerebellar body and valvula, and superficial layers of the facial and vagal lobes. In the spinal cord, klf7- and klf6a-expressing cells are found in both the dorsal and ventral horns. Numerous sensory structures (e.g. auditory, lateral line, olfactory and visual) and several motor nuclei (e.g. oculomotor, trigeminal, and vagal motor nuclei) contain klf7- and/or klf6a-expressing cells. Our results may provide useful information for determining these Klfs in maintenance and/or function in adult CNS.
authors.affiliation: Department of Biology and Integrated Bioscience Program, University of Akron, Akron, OH 44325, United States.
authors.forename: Sunil
authors.initials: S
authors.lastname: Bhattarai
authors.affiliation: Department of Biology and Integrated Bioscience Program, University of Akron, Akron, OH 44325, United States.
authors.forename: Alicja
authors.initials: A
authors.lastname: Sochacka-Marlowe
authors.affiliation: Department of Biology and Integrated Bioscience Program, University of Akron, Akron, OH 44325, United States.
authors.forename: Gerald
authors.initials: G
authors.lastname: Crutchfield
authors.affiliation: Department of Biology and Integrated Bioscience Program, University of Akron, Akron, OH 44325, United States.
authors.forename: Ramisha
authors.initials: R
authors.lastname: Khan
authors.affiliation: Department of Biology and Integrated Bioscience Program, University of Akron, Akron, OH 44325, United States.
authors.forename: Richard
authors.initials: R
authors.lastname: Londraville
authors.affiliation: Department of Biology and Integrated Bioscience Program, University of Akron, Akron, OH 44325, United States. Electronic address: qliu@uakron.edu.
authors.forename: Qin
authors.initials: Q
authors.lastname: Liu
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D002490:Central Nervous System / Q000378:metabolism*; D002531:Cerebellum / Q000378:metabolism; D005786:Gene Expression Regulation / Q000235:genetics; D017403:In Situ Hybridization; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D009474:Neurons / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D013116:Spinal Cord / Q000378:metabolism*; D013687:Telencephalon / Q000378:metabolism; D013788:Thalamus / Q000378:metabolism; D015027:Zebrafish / Q000235:genetics*; D029961:Zebrafish Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C525954:KLF6a protein, zebrafish; C525955:KLF7a protein, zebrafish; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D029961:Zebrafish Proteins
keywords: Auditory nuclei; Brain; Spinal cord; Visual structures; Zinc-finger binding transcription factors