title: Generation of KCL036 research grade human embryonic stem cell line carrying a mutation in the HTT gene.
abstract: The KCL036 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting one allele of the HTT gene encoding huntingtin (38 trinucleotide repeats; 14 for the normal allele). The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro and in vivo assays.
authors.affiliation: Stem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guys' Hospital, London, United Kingdom.
authors.forename: Laureen
authors.initials: L
authors.lastname: Jacquet
authors.affiliation: Stem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guys' Hospital, London, United Kingdom.
authors.forename: Heema
authors.initials: H
authors.lastname: Hewitson
authors.affiliation: Stem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guys' Hospital, London, United Kingdom.
authors.forename: Victoria
authors.initials: V
authors.lastname: Wood
authors.affiliation: Stem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guys' Hospital, London, United Kingdom.
authors.forename: Neli
authors.initials: N
authors.lastname: Kadeva
authors.affiliation: Stem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guys' Hospital, London, United Kingdom.
authors.forename: Glenda
authors.initials: G
authors.lastname: Cornwell
authors.affiliation: Stem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guys' Hospital, London, United Kingdom.
authors.forename: Stefano
authors.initials: S
authors.lastname: Codognotto
authors.affiliation: Histology Laboratory, Wolfson Centre for Age-Related Diseases, Faculty of Life Sciences and Medicine, King's College London, London, United Kingdom.
authors.forename: Carl
authors.initials: C
authors.lastname: Hobbs
authors.affiliation: Stem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guys' Hospital, London, United Kingdom.
authors.forename: Emma
authors.initials: E
authors.lastname: Stephenson
authors.affiliation: Stem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guys' Hospital, London, United Kingdom.
authors.forename: Dusko
authors.initials: D
authors.lastname: Ilic
mesh_terms: D000483:Alleles; D002454:Cell Differentiation; D002478:Cells, Cultured; D065150:Cellular Reprogramming; D055028:Comparative Genomic Hybridization; D004622:Embryo, Mammalian / Q000166:cytology; D005307:Fertilization in Vitro; D005838:Genotype; D000066449:Human Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D000071058:Huntingtin Protein / Q000235:genetics*; D059785:Karyotype; D008297:Male; D008856:Microscopy, Fluorescence; D010375:Pedigree; D011110:Polymorphism, Genetic; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D018911:Trinucleotide Repeats / Q000235:genetics; D020107:Troponin T / Q000378:metabolism
chemical_list: C086055:HTT protein, human; D000071058:Huntingtin Protein; C545568:TNNT2 protein, human; D014157:Transcription Factors; D020107:Troponin T