title: PCR-based Approaches for the Detection of Clinical Methicillin-resistant Staphylococcus aureus.
abstract: Staphylococcus aureus is an important pathogen that can cause a variety of infections, including superficial and systematic infections, in humans and animals. The persistent emergence of multidrug resistant S. aureus, particularly methicillin-resistant S. aureus, has caused dramatically economic burden and concerns in the public health due to limited options of treatment of MRSA infections. In order to make a correct choice of treatment for physicians and understand the prevalence of MRSA, it is extremely critical to precisely and timely diagnose the pathogen that induces a specific infection of patients and to reveal the antibiotic resistant profile of the pathogen. In this review, we outlined different PCR-based approaches that have been successfully utilized for the rapid detection of S. aureus, including MRSA and MSSA, directly from various clinical specimens. The sensitivity and specificity of detections were pointed out. Both advantages and disadvantages of listed approaches were discussed. Importantly, an alternative approach is necessary to further confirm the detection results from the molecular diagnostic assays.
authors.affiliation: Shanghai Vocational College of Agriculture and Forestry, Shanghai, China; Department of Veterinary Biomedical Science, College of Veterinary Medicine, University of Minnesota, Saint Paul, United States.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Shanghai Vocational College of Agriculture and Forestry, Shanghai, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Veterinary Biomedical Science, College of Veterinary Medicine, University of Minnesota, Saint Paul, United States.
authors.forename: Yinduo
authors.initials: Y
authors.lastname: Ji
keywords: MRSA; MSSA; S. aureus; diagnosis PCR; multiplex PCR; real-time PCR