title: Nicotine effects on muscarinic receptor-mediated free Ca[Formula: see text] level changes in the facial nucleus following facial nerve injury.
abstract: It was suggested that muscarinic, and nicotinic receptors increase free Ca[Formula: see text] levels in the facial nerve nucleus via various channels following facial nerve injury. However, intracellular Ca[Formula: see text] overload can trigger either necrotic or apoptotic cell death. It is assumed that, following facial nerve injury, the interactions of nicotinic and muscarinic acetylcholine receptors in facial nerve nucleus may negatively regulate free Ca[Formula: see text] concentrations in the facial nerve nucleus, which provide important information for the repair and regeneration of the facial nerve. The present study investigated the regulatory effects of nicotine on muscarinic receptor-mediated free calcium ion level changes in the facial nucleus in a rat model of facial nerve injury at 7, 30, and 90 days following facial nerve injury using laser confocal microscopy. The dose-dependent regulation of nicotine on muscarinic receptor-mediated free calcium ion level changes in the facial nucleus may decrease the range of free Ca[Formula: see text] increases following facial nerve injury, which is important for nerve cell regeneration. It is concluded that the negative effects of nicotine on muscarinic receptors are related to the [Formula: see text] subtype of nicotinic receptors.
authors.affiliation: * Affiliated Hospital of Qingdao University, Qingdao 266000, People's Republic of China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Sun
authors.affiliation: † School of Public Health, Qingdao University, Qingdao 266021, People's Republic of China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhou
authors.affiliation: * Affiliated Hospital of Qingdao University, Qingdao 266000, People's Republic of China.
authors.forename: Anbing
authors.initials: A
authors.lastname: Dong
authors.affiliation: * Affiliated Hospital of Qingdao University, Qingdao 266000, People's Republic of China.
authors.forename: Wenhai
authors.initials: W
authors.lastname: Sun
authors.affiliation: * Affiliated Hospital of Qingdao University, Qingdao 266000, People's Republic of China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Zhang
authors.affiliation: * Affiliated Hospital of Qingdao University, Qingdao 266000, People's Republic of China.
authors.forename: Limin
authors.initials: L
authors.lastname: Tang
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002413:Cations, Divalent / Q000378:metabolism; D004195:Disease Models, Animal; D018450:Disease Progression; D004305:Dose-Response Relationship, Drug; D020220:Facial Nerve Injuries / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D065828:Facial Nucleus / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D005260:Female; D008297:Male; D009416:Nerve Regeneration / Q000187:drug effects; D009538:Nicotine / Q000494:pharmacology*; D018722:Nicotinic Agonists / Q000494:pharmacology*; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D011976:Receptors, Muscarinic / Q000378:metabolism*; D046509:Tissue Culture Techniques
chemical_list: D002413:Cations, Divalent; D018722:Nicotinic Agonists; D011976:Receptors, Muscarinic; D009538:Nicotine; D002118:Calcium
keywords: Facial nerve injury; facial nucleus; muscarinic receptor; neural regeneration; nicotinic receptor; peripheral nerve injury