title: Gene expression analysis of bud and leaf color in tea.
abstract: Purple shoot tea attributing to the high anthocyanin accumulation is of great interest for its wide health benefits. To better understand potential mechanisms involved in purple buds and leaves formation in tea plants, we performed transcriptome analysis of six green or purple shoot tea individuals from a F1 population using the Illumina sequencing method. Totally 292 million RNA-Seq reads were obtained and assembled into 112,233 unigenes, with an average length of 759 bp and an N50 of 1081 bp. Moreover, totally 2193 unigenes showed significant differences in expression levels between green and purple tea samples, with 1143 up- and 1050 down-regulated in the purple teas. Further real time PCR analysis confirmed RNA-Seq results. Our study identified 28 differentially expressed transcriptional factors and A CsMYB gene was found to be highly similar to AtPAP1 in Arabidopsis. Further analysis of differentially expressed genes involved in anthocyanin biosynthesis and transportation showed that the late biosynthetic genes and genes involved in anthocyanin transportation were largely affected but the early biosynthetic genes were less or none affected. Overall, the identification of a large number of differentially expressed genes offers a global view of the potential mechanisms associated with purple buds and leaves formation, which will facilitate molecular breeding in tea plants.
authors.affiliation: Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, National Center for Tea Improvement, Tea Research Institute Chinese Academy of Agricultural Sciences (TRICAAS), 9 Meiling South Road, Hangzhou, Zhejiang 310008, PR China.
authors.forename: Kang
authors.initials: K
authors.lastname: Wei
authors.affiliation: Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, National Center for Tea Improvement, Tea Research Institute Chinese Academy of Agricultural Sciences (TRICAAS), 9 Meiling South Road, Hangzhou, Zhejiang 310008, PR China.
authors.forename: Yazhen
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, National Center for Tea Improvement, Tea Research Institute Chinese Academy of Agricultural Sciences (TRICAAS), 9 Meiling South Road, Hangzhou, Zhejiang 310008, PR China.
authors.forename: Liyun
authors.initials: L
authors.lastname: Wu
authors.affiliation: Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, National Center for Tea Improvement, Tea Research Institute Chinese Academy of Agricultural Sciences (TRICAAS), 9 Meiling South Road, Hangzhou, Zhejiang 310008, PR China.
authors.forename: Hailin
authors.initials: H
authors.lastname: Li
authors.affiliation: Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, National Center for Tea Improvement, Tea Research Institute Chinese Academy of Agricultural Sciences (TRICAAS), 9 Meiling South Road, Hangzhou, Zhejiang 310008, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Ruan
authors.affiliation: Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, National Center for Tea Improvement, Tea Research Institute Chinese Academy of Agricultural Sciences (TRICAAS), 9 Meiling South Road, Hangzhou, Zhejiang 310008, PR China.
authors.forename: Peixian
authors.initials: P
authors.lastname: Bai
authors.affiliation: Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, National Center for Tea Improvement, Tea Research Institute Chinese Academy of Agricultural Sciences (TRICAAS), 9 Meiling South Road, Hangzhou, Zhejiang 310008, PR China.
authors.forename: Chengcai
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, National Center for Tea Improvement, Tea Research Institute Chinese Academy of Agricultural Sciences (TRICAAS), 9 Meiling South Road, Hangzhou, Zhejiang 310008, PR China.
authors.forename: Fen
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, National Center for Tea Improvement, Tea Research Institute Chinese Academy of Agricultural Sciences (TRICAAS), 9 Meiling South Road, Hangzhou, Zhejiang 310008, PR China.
authors.forename: Liyi
authors.initials: L
authors.lastname: Xu
authors.affiliation: Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, National Center for Tea Improvement, Tea Research Institute Chinese Academy of Agricultural Sciences (TRICAAS), 9 Meiling South Road, Hangzhou, Zhejiang 310008, PR China. Electronic address: wangly@tricaas.com.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Wang
authors.affiliation: Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, National Center for Tea Improvement, Tea Research Institute Chinese Academy of Agricultural Sciences (TRICAAS), 9 Meiling South Road, Hangzhou, Zhejiang 310008, PR China. Electronic address: chenghao@tricaas.com.
authors.forename: Hao
authors.initials: H
authors.lastname: Cheng
mesh_terms: D000595:Amino Acid Sequence; D000872:Anthocyanins / Q000737:chemistry / Q000378:metabolism; D053898:Biosynthetic Pathways / Q000235:genetics; D028241:Camellia sinensis / Q000235:genetics*; D035264:Flowers / Q000235:genetics*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D010858:Pigmentation / Q000235:genetics*; D018515:Plant Leaves / Q000235:genetics*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D016415:Sequence Alignment; D017423:Sequence Analysis, RNA; D013662:Tea / Q000235:genetics*; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation / Q000235:genetics
chemical_list: D000872:Anthocyanins; D010940:Plant Proteins; D013662:Tea; D014157:Transcription Factors
keywords: Anthocyanins; Camellia sinensis; MYB transcriptional factor; Purple soot tea; RNA-Seq analysis