title: Lytic bacteriophage PM16 specific for Proteus mirabilis: a novel member of the genus Phikmvvirus.
abstract: Lytic Proteus phage PM16, isolated from human faeces, is a novel virus that is specific for Proteus mirabilis cells. Bacteriophage PM16 is characterized by high stability, a short latency period, large burst size and the occurrence of low phage resistance. Phage PM16 was classified as a member of the genus Phikmvvirus on the basis of genome organization, gene synteny, and protein sequences similarities. Within the genus Phikmvvirus, phage PM16 is grouped with Vibrio phage VP93, Pantoea phage LIMElight, Acinetobacter phage Petty, Enterobacter phage phiKDA1, and KP34-like bacteriophages. An investigation of the phage-cell interaction demonstrated that phage PM16 attached to the cell surface, not to the bacterial flagella. The study of P. mirabilis mutant cells obtained during the phage-resistant bacterial cell assay that were resistant to phage PM16 re-infection revealed a non-swarming phenotype, changes in membrane characteristics, and the absence of flagella. Presumably, the resistance of non-swarming P. mirabilis cells to phage PM16 re-infection is determined by changes in membrane macromolecular composition and is associated with the absence of flagella and a non-swarming phenotype.
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Lavrentieva Ave., 8, Novosibirsk, Russia. morozova@niboch.nsc.ru.
authors.forename: V
authors.identifier: http://orcid.org/0000-0002-0869-3476
authors.initials: V
authors.lastname: Morozova
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Lavrentieva Ave., 8, Novosibirsk, Russia.
authors.forename: Yu
authors.initials: Y
authors.lastname: Kozlova
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Lavrentieva Ave., 8, Novosibirsk, Russia.
authors.forename: E
authors.initials: E
authors.lastname: Shedko
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Lavrentieva Ave., 8, Novosibirsk, Russia.
authors.forename: A
authors.initials: A
authors.lastname: Kurilshikov
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Lavrentieva Ave., 8, Novosibirsk, Russia.
authors.forename: I
authors.initials: I
authors.lastname: Babkin
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Lavrentieva Ave., 8, Novosibirsk, Russia.
authors.forename: A
authors.initials: A
authors.lastname: Tupikin
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Lavrentieva Ave., 8, Novosibirsk, Russia.
authors.forename: A
authors.initials: A
authors.lastname: Yunusova
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Lavrentieva Ave., 8, Novosibirsk, Russia.
authors.forename: A
authors.initials: A
authors.lastname: Chernonosov
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Lavrentieva Ave., 8, Novosibirsk, Russia.
authors.forename: I
authors.initials: I
authors.lastname: Baykov
authors.affiliation: Limnological Institute of SB RAS, Ulan-Batorskaya Str., 3, Irkutsk, Russia.
authors.forename: I
authors.initials: I
authors.lastname: Кondratov
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Lavrentieva Ave., 8, Novosibirsk, Russia.
authors.forename: M
authors.initials: M
authors.lastname: Kabilov
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Lavrentieva Ave., 8, Novosibirsk, Russia.
authors.forename: E
authors.initials: E
authors.lastname: Ryabchikova
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Lavrentieva Ave., 8, Novosibirsk, Russia.
authors.forename: V
authors.initials: V
authors.lastname: Vlassov
authors.affiliation: Institute of Chemical Biology and Fundamental Medicine, Lavrentieva Ave., 8, Novosibirsk, Russia.
authors.forename: N
authors.initials: N
authors.lastname: Tikunova
mesh_terms: D001435:Bacteriophages / Q000145:classification / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D016000:Cluster Analysis; D016679:Genome, Viral; D010802:Phylogeny; D011513:Proteus mirabilis / Q000821:virology*; D010948:Viral Plaque Assay; D014779:Virus Replication / Q000502:physiology