title: Determination the Usefulness ofAhHMA4p1::AhHMA4Expression in Biofortification Strategies.
abstract: AhHMA4fromArabidopsis thalianaencodes Zn/Cd export protein that controls Zn/Cd translocation to shoots. The focus of this manuscript is the evaluation ofAhHMA4expression in tomato for mineral biofortification (more Zn and less Cd in shoots and fruits). Hydroponic and soil-based experiments were performed. Transgenic and wild-type plants were grown on two dilution levels of Knop's medium (1/10, 1/2) with or without Cd, to determine if mineral composition affects the pattern of root/shoot partitioning of both metals due toAhHMA4expression. Facilitation of Zn translocation to shoots of 19-day-old transgenic tomato was noted only when plants were grown in the more diluted medium. Moreover, the expression pattern of Zn-Cd-Fe cross-homeostasis genes (LeIRT1,LeChln,LeNRAMP1) was changed in transgenics in a medium composition-dependent fashion. In plants grown in soil (with/without Cd) up to maturity, expression ofAhHMA4resulted in more efficient translocation of Zn to shoots and restriction of Cd. These results indicate the usefulness ofAhHMA4expression to improve the growth of tomato on low-Zn soil, also contaminated with Cd.
authors.affiliation: Faculty of Biology, Institute of Experimental Plant Biology and Biotechnology, University of Warsaw, Miecznikowa str 1, 02-096 Warszawa, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Weremczuk
authors.affiliation: Faculty of Biology, Institute of Experimental Plant Biology and Biotechnology, University of Warsaw, Miecznikowa str 1, 02-096 Warszawa, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Barabasz
authors.affiliation: Faculty of Chemistry, University of Warsaw, Pasteura str. 1, 02-093 Warszawa, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Ruszczyńska
authors.affiliation: Faculty of Chemistry, University of Warsaw, Pasteura str. 1, 02-093 Warszawa, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Bulska
authors.affiliation: Faculty of Biology, Institute of Experimental Plant Biology and Biotechnology, University of Warsaw, Miecznikowa str 1, 02-096 Warszawa, Poland.
authors.forename: Danuta Maria
authors.initials: DM
authors.lastname: Antosiewicz
keywords: Biofortification; Cadmium; HMA4; Tomato; Transformation; Zinc