title: Peripheral blood mononuclear cell-converted induced pluripotent stem cells (iPSCs) from an early onset Alzheimer's patient.
abstract: Improvement in transduction efficiency makes it possible to convert blood cells into induced pluripotent stem cells (iPSC). In this study, we generated an iPSC line from peripheral blood mononuclear cells (PBMC) donated by a patient who exhibited memory deficit at age 59; outcome of positron emission tomography scan is consistent with a diagnosis of Alzheimer's disease. Integration-free CytoTune-iPS Sendai Reprogramming factors which include Sendai virus particles of the four Yamanaka factors Oct4, Sox2, Klf4, and c-Myc were introduced to PBMC to convert them to iPSCs without retention of virus. Three germ layer differentiation was induced to demonstrate the pluripotency of these iPSCs.
authors.affiliation: Department of Neurology, Rhode Island Hospital and Brown University Warren Alpert Medical School, 593 Eddy Street, Providence, RI, United States; Geriatric Research, Education and Clinical Center, Edith Nourse Rogers Memorial Veterans Hospital, Bedford, MA, United States.
authors.forename: Han-Kyu
authors.initials: HK
authors.lastname: Lee
authors.affiliation: Geriatric Research, Education and Clinical Center, Edith Nourse Rogers Memorial Veterans Hospital, Bedford, MA, United States.
authors.forename: Peter
authors.initials: P
authors.lastname: Morin
authors.affiliation: Geriatric Research, Education and Clinical Center, Edith Nourse Rogers Memorial Veterans Hospital, Bedford, MA, United States. Electronic address: weiming.xia@va.gov.
authors.forename: Weiming
authors.initials: W
authors.lastname: Xia
mesh_terms: D000544:Alzheimer Disease / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology*; D002454:Cell Differentiation; D065150:Cellular Reprogramming; D058732:Embryoid Bodies / Q000166:cytology; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D059785:Karyotype; D000090062:Kruppel-Like Factor 4; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D008297:Male; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D049268:Positron-Emission Tomography; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: C000715467:KLF4 protein, human; D000090062:Kruppel-Like Factor 4; D014157:Transcription Factors