title: Quantitative Detection of MicroRNA in One Step via Next Generation Magnetic Relaxation Switch Sensing.
abstract: One-step, quantitative and rapid detection of microRNA (miRNA) in tumor cells or tissues can provide critical information for clinical diagnosis and cancer treatment. In this work, we develop a magnetic relaxation switch sensing (MRS)-based miRNA sensor using magnetic microparticle (1 μm in diameter, MM1000)-DNA probe-magnetic nanoparticle (30 nm in diameter, MN30) conjugates (MM1000-DNA-MN30). In the presence of target miRNA, DSN enzyme selectively cleaves the DNA tether after miRNA/DNA hybridization to release MN30 and leaves the miRNA intact to lead to the declustering of more MN30 than before. In contrast to conventional MRS by measuring the change of transverse relaxation time (ΔT2) induced by the aggregation or dissociation of magnetic particles in the presence of target, we use the cleaved MN30 from conjugates as the direct readout of ΔT2, which is more sensitive and stable. This MRS-based assay allows for one-step detection of 5 fM of miR-21 in urine samples, quantification of miR-21 from 100 cancer cells, and differentiation of the expression of miR-21 in tumor and surrounding tissues. The merits of this assay, rapidity, ability for quantitation, high sensitivity, and one-step operation, ensure a promising future in diagnostic technology.
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology & CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for NanoScience and Technology , Beijing 100190, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Lu
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology & CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for NanoScience and Technology , Beijing 100190, China.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Institute of Blood Transfusion, Chinese Academy of Medical Sciences (CAMS)/Peking Union Medical College (PUMC) , Beijing 100730, China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Liu
authors.affiliation: The Department of Hepatopancreaticobiliary Surgical Oncology, Chinese PLA General Hospital , Beijing 100853, China.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Tang
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology & CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for NanoScience and Technology , Beijing 100190, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Feng
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology & CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for NanoScience and Technology , Beijing 100190, China.
authors.forename: Jiashu
authors.initials: J
authors.lastname: Sun
authors.affiliation: Beijing Engineering Research Center for BioNanotechnology & CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for NanoScience and Technology , Beijing 100190, China.
authors.forename: Xingyu
authors.initials: X
authors.lastname: Jiang
mesh_terms: D004247:DNA; D015342:DNA Probes; D035683:MicroRNAs / Q000032:analysis*; D053758:Nanoparticles; D009369:Neoplasms / Q000737:chemistry; D009693:Nucleic Acid Hybridization
chemical_list: D015342:DNA Probes; D035683:MicroRNAs; D004247:DNA
keywords: detection; magnetic nanoparticles; magnetic relaxation switch; miRNA; tumor