title: Evaluation and validity of a polymerase chain reaction-based open reading frame typing method to dissect the molecular epidemiology for Acinetobacter baumannii in an epidemiologic study of a hospital outbreak.
abstract: Acinetobacter baumannii is regarded as one of the most important pathogens in hospital outbreaks. To obtain an efficient and simple epidemiologic method of surveillance during outbreaks, we assessed the applicability of the polymerase chain reaction-based open reading frames typing (POT) method and compared it with pulsed-field gel electrophoresis. The POT method was found to have sufficient discriminatory power to identify the strains and would be widely applicable to epidemiologic surveillance during hospital outbreaks.
authors.affiliation: Department of Microbiology, Tokyo Medical University, Tokyo, Japan; Division of Infectious Diseases and Respiratory Medicine, National Defense Medical College, Saitama, Japan. Electronic address: fujikura@ndmc.ac.jp.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Fujikura
authors.affiliation: Department of Clinical Laboratory, National Defense Medical College Hospital, Saitama, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yuki
authors.affiliation: Department of Clinical Laboratory, National Defense Medical College Hospital, Saitama, Japan.
authors.forename: Takaaki
authors.initials: T
authors.lastname: Hamamoto
authors.affiliation: Department of Bacteriology, BML, Inc., Saitama, Japan.
authors.forename: Sadahiro
authors.initials: S
authors.lastname: Ichimura
authors.affiliation: Division of Infectious Diseases and Respiratory Medicine, National Defense Medical College, Saitama, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kawana
authors.affiliation: Department of Microbiology, Tokyo Medical University, Tokyo, Japan.
authors.forename: Kiyofumi
authors.initials: K
authors.lastname: Ohkusu
authors.affiliation: Department of Microbiology, Tokyo Medical University, Tokyo, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Matsumoto
mesh_terms: D000151:Acinetobacter Infections / Q000453:epidemiology* / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D003428:Cross Infection / Q000453:epidemiology* / Q000382:microbiology; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D017720:Molecular Epidemiology / Q000379:methods; D058889:Molecular Typing / Q000379:methods*; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction / Q000379:methods*
keywords: Acinetobacter baumannii; PCR-based open reading frames typing (POT) method; epidemiology; outbreak; pulsed-field gel electrophoresis