title: The Tumor Suppressor MicroRNA-1 Exhibits Restricted Inhibition of Proliferation of Ovarian Cancer Cells.
abstract: BACKGROUND
  MicroRNAs are able to control vital tumor biological processes, such as proliferation, tissue transformation and cell migration, as well as apoptosis. One of the micro RNAs, namely miR-1, has been classified as a tumor suppressor, however, preliminary data did not confirm this finding in ovarian cancer (OC) cells. This study examined the impact of miR-1 on OC cell growth.
  
  
  METHODS
  Recombinant miR-1 was overexpressed in human OC cell lines OVCAR-3, SK-OV-3, TOV-112D, and TOV-21G. Subsequently, cell growth was analyzed.
  
  
  RESULTS
  After transfection, 11- to 487-fold overexpression of miR-1 was detectable in the OC cells. However, no significant differences in proliferation compared to control cells were detected, neither in transiently nor in stably transfected cells.
  
  
  CONCLUSIONS
  In numerous cancer entities miR-1 is defined as an antiproliferative tumor suppressor. Notably, the present study demonstrated a loss of growth-inhibitory functionality of miR-1 by so far unknown mechanisms, suggesting dysregulated miR-1 signaling or effector cascades in OC cells.
authors.affiliation: Department of Urology, University of Medicine Greifswald, Greifswald, Germany matthias.stope@uni-greifswald.de.
authors.forename: Matthias B
authors.initials: MB
authors.lastname: Stope
authors.affiliation: Department of Gynecology and Obstetrics, University of Medicine Greifswald, Greifswald, Germany.
authors.forename: Daria
authors.initials: D
authors.lastname: Hettenbach
authors.affiliation: Department of Gynecology and Obstetrics, University of Medicine Greifswald, Greifswald, Germany.
authors.forename: Anne
authors.initials: A
authors.lastname: Kaul
authors.affiliation: Department of Gynecology and Obstetrics, University of Medicine Greifswald, Greifswald, Germany.
authors.forename: Madeleine
authors.initials: M
authors.lastname: Paditz
authors.affiliation: Department of Gynecology and Obstetrics, University of Medicine Greifswald, Greifswald, Germany.
authors.forename: Karoline
authors.initials: K
authors.lastname: Diesing
authors.affiliation: Department of Urology, University of Medicine Greifswald, Greifswald, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Burchardt
authors.affiliation: Department of Gynecology and Obstetrics, University of Medicine Greifswald, Greifswald, Germany.
authors.forename: Marek
authors.initials: M
authors.lastname: Zygmunt
authors.affiliation: Department of Gynecology and Obstetrics, University of Medicine Greifswald, Greifswald, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Mustea
authors.affiliation: Department of Gynecology and Obstetrics, University of Medicine Greifswald, Greifswald, Germany.
authors.forename: Dominique
authors.initials: D
authors.lastname: Koensgen
mesh_terms: D016022:Case-Control Studies; D048708:Cell Growth Processes / Q000235:genetics; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D035683:MicroRNAs / Q000008:administration & dosage* / Q000096:biosynthesis / Q000235:genetics; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D014162:Transfection
chemical_list: C544547:MIRN1 microRNA, human; D035683:MicroRNAs
keywords: Ovarian cancer; microRNA miR-1; proliferation; tumor suppressor