title: Comparison of leucine-rich repeat-containing G protein-coupled receptor 5 expression in different cancer and normal cell lines.
abstract: Evaluating the expression of leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5) may be useful for predicting the best models and achieving more accurate results in cancer research. Therefore, the aim of the present study was to analyze the LGR5 expression levels in different cell lines. Eight commonly used cell lines were assessed (COS-7, NIH3T3, HEK293, VERO, HeLa, BHK, HepG2 and AGS). All the cell lines were cultured in RPMI-1640 medium contain 10% fetal calf serum at 37°C in humidified conditions with 5% CO2. According to the western blotting results, LGR5 was expressed in all cell lines. Densitometry results of LGR5 expression in the different cell lines showed that high LGR5 expression levels were apparent in BHK, AGS, VERO and NIH3T3 cell lines compared with the other cell lines. The results indicate that for the normal and cancer cell lines, BNK and AGS may be a better choice, respectively, forin vitrocancer studies.
authors.affiliation: Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari 48175-861, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Alizadeh-Navaei
authors.affiliation: Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari 48175-861, Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Rafiei
authors.affiliation: Immunogenetic Research Center, Mazandaran University of Medical Sciences, Sari 48175-861, Iran.
authors.forename: Saeid
authors.initials: S
authors.lastname: Abedian-Kenari
authors.affiliation: Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari 48175-861, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Asgarian-Omran
authors.affiliation: Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari 48175-861, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Valadan
authors.affiliation: Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari 48175-861, Iran.
authors.forename: Akbar
authors.initials: A
authors.lastname: Hedayatizadeh-Omran
keywords: cancer cell lines; leucine-rich repeat-containing G protein-coupled receptor 5 expression; western blotting