title: Human embryonic stem cells derived from abnormal blastocyst donated by glucose-6-phosphate dehydrogenase deficiency patient.
abstract: A human embryonic stem cell (hESC) line was derived from abnormal embryo donated by Glucose-6-phosphate dehydrogenase (G6PD) deficiency patient. Sequencing analysis confirmed that the hESC line possessed the mutant contributing to abnormal expression of G6PD. Further characteristic analysis demonstrated that the favism hESC line maintained stable and normal karyotype, expressed pluripotent markers and had the capacity of generating the derivatives from all three germ layers.
authors.affiliation: Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhou
authors.affiliation: National Engineering and Research Center of Human Stem Cell, Changsha, China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China.
authors.forename: Long
authors.initials: L
authors.lastname: Li
authors.affiliation: Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China; Reproductive & Genetic Hospital of CITIC-Xiangya, Changsha, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.affiliation: National Engineering and Research Center of Human Stem Cell, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China; Reproductive & Genetic Hospital of CITIC-Xiangya, Changsha, China.
authors.forename: Guangxiu
authors.initials: G
authors.lastname: Lu
authors.affiliation: Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cell, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China; Reproductive & Genetic Hospital of CITIC-Xiangya, Changsha, China.
authors.forename: Ge
authors.initials: G
authors.lastname: Lin
authors.affiliation: Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cell, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China. Electronic address: loretta0730@163.com.
authors.forename: Qi
authors.initials: Q
authors.lastname: Ouyang
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D001755:Blastocyst / Q000473:pathology*; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002460:Cell Line; D005955:Glucosephosphate Dehydrogenase Deficiency / Q000473:pathology*; D000066449:Human Embryonic Stem Cells / Q000166:cytology*; D006801:Humans; D007621:Karyotyping; D016513:Mice, SCID; D015203:Reproducibility of Results; D014019:Tissue Donors
chemical_list: D000469:Alkaline Phosphatase