title: Effect of IL6 and IL23 on double negative T cells and anti ds-DNA in systemic lupus erythematosus patients.
abstract: Several evidences suggest that DN T cells, IL23 and IL6 play a role in the pathogenesis of SLE. This study aimed to evaluate the frequency of DN T cells in SLE patients and the relation to their activity also to assess the possible role of IL6 and IL23 on DN T cells. Thirty patients with SLE and sixteen healthy blood donor females were enrolled. There was a significant increase in DN T cells in patients than controls (P=0.001). These cells had a significant positive correlation with SLEDAI (r=0.486, P=0.006). DN T cells from SLE patient samples were expanded when stimulated in vitro with RhIL6 or RhIL23 in patients than controls. Furthermore, anti ds-DNA level was found to be increased in supernatant of PBMCs when stimulated by these cytokines in different concentrations. Our findings suggest that IL6 and IL23 may play role in SLE pathogenesis through their effect on DN T cells and anti ds-DNA.
authors.affiliation: Medical Microbiology & Immunology, Faculty of Medicine Assiut University, Assiut, Egypt.
authors.forename: Asmaa S
authors.initials: AS
authors.lastname: Shaltout
authors.affiliation: Department of Clinical Pathology, South Egypt Cancer Institute, Assiut University, Egypt. Electronic address: douaa_sayed@hotmail.com.
authors.forename: Douaa
authors.initials: D
authors.lastname: Sayed
authors.affiliation: Medical Microbiology & Immunology, Faculty of Medicine Assiut University, Assiut, Egypt.
authors.forename: Mohamed S
authors.initials: MS
authors.lastname: Badary
authors.affiliation: Medical Microbiology & Immunology, Faculty of Medicine Assiut University, Assiut, Egypt.
authors.forename: Amany M
authors.initials: AM
authors.lastname: Nafee
authors.affiliation: Internal Medicine, Rheumatology Unit, Assiut University, Assiut, Egypt.
authors.forename: Mona H
authors.initials: MH
authors.lastname: El Zohri
authors.affiliation: Department of Clinical Pathology, South Egypt Cancer Institute, Assiut University, Egypt.
authors.forename: Rania
authors.initials: R
authors.lastname: Bakry
authors.affiliation: Medical Microbiology & Immunology, Faculty of Medicine Assiut University, Assiut, Egypt.
authors.forename: Shabaan H
authors.initials: SH
authors.lastname: Ahmed
mesh_terms: D000293:Adolescent; D000328:Adult; D000974:Antibodies, Antinuclear / Q000378:metabolism; D015704:CD4 Antigens / Q000378:metabolism; D016827:CD8 Antigens / Q000378:metabolism; D016022:Case-Control Studies; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D018450:Disease Progression; D005260:Female; D006801:Humans; D053759:Interleukin-23 / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism*; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008213:Lymphocyte Activation; D013601:T-Lymphocytes / Q000276:immunology*; D055815:Young Adult
chemical_list: D000974:Antibodies, Antinuclear; D015704:CD4 Antigens; D016827:CD8 Antigens; D053759:Interleukin-23; D015850:Interleukin-6
keywords: DN T cells; IL23; SLE