title: Persistence and Elimination of Human Norovirus in Food and on Food Contact Surfaces: A Critical Review.
abstract: This critical review addresses the persistence of human norovirus (NoV) in water, shellfish, and processed meats; on berries, herbs, vegetables, fruits, and salads; and on food contact surfaces. The review focuses on studies using NoV; information from studies involving only surrogates is not included. It also addresses NoV elimination or inactivation by various chemical, physical, or processing treatments. In most studies, persistence or elimination was determined by detection and quantification of the viral genome, although improved methods for determining infectivity have been proposed. NoV persisted for 60 to 728 days in water, depending on water source. It also persisted on berries, vegetables, and fruit, often showing <1-log reduction within 1 to 2 weeks. NoV was resilient on carpets, Formica, stainless steel, polyvinyl chloride, and ceramic surfaces; during shellfish depuration; and to repeated freeze-thaw cycles. Copper alloy surfaces may inactivate NoV by damaging viral capsids. Disinfection was achieved for some foods or food contact surfaces using chlorine, calcium or sodium hypochlorite, chlorine dioxide, high hydrostatic pressure, high temperatures, pH values >8.0, freeze-drying, and UV radiation. Ineffective disinfectants included hydrogen peroxide, quaternary ammonium compounds, most ethanol-based disinfectants, and antiseptics at normally used concentrations. Thorough washing of herbs and produce was effective in reducing, but not eliminating, NoV in most products. Washing hands with soap generally reduced NoV by <2 log. Recommendations for future research needs are provided.
authors.affiliation: Food and Environment Research Agency, Sand Hutton, York, YO41 1LZ, UK.
authors.forename: Nigel
authors.initials: N
authors.lastname: Cook
authors.affiliation: Leatherhead Food Research, Leatherhead, Surrey, KT22 7RY, UK.
authors.forename: Angus
authors.initials: A
authors.lastname: Knight
authors.affiliation: U.S. Department of Agriculture, Agricultural Research Service, Dover, Delaware 19901, USA. gary.richards@ars.usda.gov.
authors.forename: Gary P
authors.initials: GP
authors.lastname: Richards
mesh_terms: D017606:Chlorine Compounds / Q000494:pharmacology; D004202:Disinfectants / Q000494:pharmacology; D005516:Food Microbiology; D006801:Humans; D029322:Norovirus / Q000187:drug effects*; D010087:Oxides; D038301:Virus Inactivation / Q000187:drug effects*
chemical_list: D017606:Chlorine Compounds; D004202:Disinfectants; D010087:Oxides; C025109:chlorine dioxide