title: Involvement of microRNA-1297, a new regulator of HMGA1, in the regulation of glioma cell growth in vivo and in vitro.
abstract: MicroRNAs (miRNAs) are a class of versatile gene expression regulators, participating in the regulation of gene expression at the post-transcriptional level in both physiological and pathological conditions. Gliomas are the most common brain malignancy in adults, and deregulation of microRNAs takes part in the gliomagenesis process. Here, we found that the expression of miR-1297 is significantly reduced in both glioma cell lines and clinical glioma tissues. Using the MTT assay, soft agar colony formation assay and xenograft tumor formation assay, we show that miR-1297 is a tumor suppressor microRNA in gliomas. We demonstrate that the high mobility group protein A1 (HMGA1) is the functional target of miR-1297 in glioma cells. HMGA1 significantly promotes the growth of glioma cells both in vitro and in vivo. Together, we unveil a new molecular mechanism in gliomas that may shed new light on understanding this brain malignancy.
authors.affiliation: Tianjin Neurological Institute, Department of Neurosurgery, General Hospital, Tianjin Medical UniversityTianjin 300052, China; Department of Neurosurgery, The People's Hospital of Hainan ProvinceHaikou 570311, Hainan, China.
authors.forename: Jiachong
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, The People's Hospital of Hainan Province Haikou 570311, Hainan, China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Neurosurgery, The People's Hospital of Hainan Province Haikou 570311, Hainan, China.
authors.forename: Shaowei
authors.initials: S
authors.lastname: Mo
authors.affiliation: Tianjin Neurological Institute, Department of Neurosurgery, General Hospital, Tianjin Medical University Tianjin 300052, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Department of Laboratory Medicine, The First People's Hospital of Yancheng City Yancheng 224005, Jiangsu, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.affiliation: Tianjin Neurological Institute, Department of Neurosurgery, General Hospital, Tianjin Medical University Tianjin 300052, China.
authors.forename: Jianning
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, The People's Hospital of Hainan Province Haikou 570311, Hainan, China.
authors.forename: Jiannong
authors.initials: J
authors.lastname: Zhao
keywords: HMGA1; cell growth; glioma; miR-1297