title: Neuroprotective actions of taurine on hypoxic-ischemic brain damage in neonatal rats.
abstract: Taurine is an abundant amino acid in the nervous system, which has been proved to possess antioxidation, osmoregulation and membrane stabilization. Previously it has been demonstrated that taurine exerts ischemic brain injury protective effect. This study was designed to investigate whether the protective effect of taurine has the possibility to be applied to treat neonatal hypoxic-ischemic brain damage. Seven-day-old Sprague-Dawley rats were treated with left carotid artery ligation followed by exposure to 8% oxygen to generate the experimental group. The cerebral damage area was measured after taurine post-treatment with 2,3,5-triphenyltetrazolium chloride (TTC) staining, Hematoxyline-Eosin (HE) staining and Nissl staining. The activities of superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-Px), total antioxidant capacity (T-AOC), myeloperoxtidase (MPO), ATP and Lactic Acid productions were assayed with ipsilateral hemisphere homogenates. Western-blot and immunofluorescence assay were processed to detect the expressions of AIF, Cyt C, Bax, Bcl-2 in brain. We found that taurine significantly reduced brain infarct volume and ameliorated morphological injury obviously reversed the changes of SOD, MDA, GSH-Px, T-AOC, ATP, MPO, and Lactic Acid levels. Compared with hypoxic-ischemic group, it showed marked reduction of AIF, Cyt C and Bax expressions and increase of Bcl-2 after post-treatment. We conclude that taurine possesses an efficacious neuroprotective effect after cerebral hypoxic-ischemic damage in neonatal rats.
authors.affiliation: Department of Pharmacology, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region 750004, PR China.
authors.forename: Xiao-Yun
authors.initials: XY
authors.lastname: Zhu
authors.affiliation: Department of Pharmacology, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region 750004, PR China.
authors.forename: Peng-Sheng
authors.initials: PS
authors.lastname: Ma
authors.affiliation: Department of Pharmacology, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region 750004, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.affiliation: Department of Pharmacology, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region 750004, PR China.
authors.forename: Ru
authors.initials: R
authors.lastname: Zhou
authors.affiliation: Department of Pharmacology, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region 750004, PR China.
authors.forename: Yin-Ju
authors.initials: YJ
authors.lastname: Hao
authors.affiliation: Key Laboratory of Hui Ethnic Medicine Modernization, Ministry of Education, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region 750004, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Niu
authors.affiliation: Ningxia Key Laboratory of Craniocerebral Diseases of Ningxia Hui Autonomous Region, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region 750004, PR China.
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.affiliation: College of Nursing, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region 750004, PR China. Electronic address: li_yuxiang@163.com.
authors.forename: Yu-Xiang
authors.initials: YX
authors.lastname: Li
authors.affiliation: Department of Pharmacology, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region 750004, PR China; Ningxia Hui Medicine Modern Engineering Research Center and Collaborative Innovation Center, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region 750004, PR China. Electronic address: yujq910315@163.com.
authors.forename: Jian-Qiang
authors.initials: JQ
authors.lastname: Yu
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D051033:Apoptosis Inducing Factor / Q000378:metabolism; D020520:Brain Infarction / Q000209:etiology / Q000517:prevention & control; D001930:Brain Injuries / Q000209:etiology* / Q000517:prevention & control*; D005260:Female; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D020925:Hypoxia-Ischemia, Brain / Q000150:complications*; D019344:Lactic Acid / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D018384:Oxidative Stress / Q000187:drug effects; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D013654:Taurine / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D051033:Apoptosis Inducing Factor; D018696:Neuroprotective Agents; D019253:Proto-Oncogene Proteins c-bcl-2; D013654:Taurine; D019344:Lactic Acid; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005978:Glutathione
keywords: Cell death; Hypoxic-ischemic; Neonatal; Neuroprotection; Oxidative stress; Taurine