title: TCR-contacting residues orientation and HLA-DRβ* binding preference determine long-lasting protective immunity against malaria.
abstract: Fully-protective, long-lasting, immunological (FPLLI) memory against Plasmodium falciparum malaria regarding immune protection-inducing protein structures (IMPIPS) vaccinated into monkeys previously challenged and re-challenged 60 days later with a lethal Aotus monkey-adapted P. falciparum strain was found to be associated with preferential high binding capacity to HLA-DRβ1* allelic molecules of the major histocompatibility class II (MHC-II), rather than HLA-DRβ3*, β4*, β5* alleles. Complete PPIIL 3D structure, a longer distance (26.5 Å ± 1.5 Å) between residues perfectly fitting into HLA-DRβ1*PBR pockets 1 and 9, a gauche(-) rotamer orientation in p8 TCR-contacting polar residue and a larger volume of polar p2 residues was also found. This data, in association with previously-described p3 and p7 apolar residues having gauche(+) orientation to form a perfect MHC-II-peptide-TCR complex, determines the stereo-electronic and topochemical characteristics associated with FPLLI immunological memory.
authors.affiliation: Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá D. C., Colombia; Universidad del Rosario, Bogotá D. C., Colombia; Universidad de Ciencias Aplicadas y Ambientales (UDCA), Bogotá, Colombia.
authors.forename: Martha P
authors.initials: MP
authors.lastname: Alba
authors.affiliation: Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá D. C., Colombia; Universidad del Rosario, Bogotá D. C., Colombia; Universidad de Ciencias Aplicadas y Ambientales (UDCA), Bogotá, Colombia.
authors.forename: Carlos F
authors.initials: CF
authors.lastname: Suarez
authors.affiliation: Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá D. C., Colombia.
authors.forename: Yahson
authors.initials: Y
authors.lastname: Varela
authors.affiliation: Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá D. C., Colombia; Universidad del Rosario, Bogotá D. C., Colombia.
authors.forename: Manuel A
authors.initials: MA
authors.lastname: Patarroyo
authors.affiliation: Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá D. C., Colombia; Universidad del Rosario, Bogotá D. C., Colombia.
authors.forename: Adriana
authors.initials: A
authors.lastname: Bermudez
authors.affiliation: Fundación Instituto de Inmunología de Colombia (FIDIC), Bogotá D. C., Colombia; Universidad Nacional de Colombia, Bogotá D. C., Colombia. Electronic address: mepatarr@gmail.com.
authors.forename: Manuel E
authors.initials: ME
authors.lastname: Patarroyo
mesh_terms: D000818:Animals; D001029:Aotus trivirgatus; D001665:Binding Sites; D059806:HLA-DR beta-Chains / Q000737:chemistry* / Q000276:immunology*; D007113:Immunity, Innate / Q000276:immunology; D007156:Immunologic Memory / Q000276:immunology; D008288:Malaria / Q000276:immunology*; D010963:Plasmodium falciparum / Q000276:immunology*; D011485:Protein Binding; D011948:Receptors, Antigen, T-Cell / Q000737:chemistry* / Q000276:immunology*; D013329:Structure-Activity Relationship
chemical_list: D059806:HLA-DR beta-Chains; D011948:Receptors, Antigen, T-Cell
keywords: Antimalarial-vaccine; Immunological memory; MHC-II; Rotamer-orientation; T-cell-receptor