title: Characterization of 19 microsatellite loci in the clonal monkshood Aconitum kusnezoffii (Ranunculaceae).
abstract: OBJECTIVE
  Microsatellite loci were isolated and characterized from Aconitum kusnezoffii (Ranunculaceae) to estimate male and female reproductive success and evaluate the effects of clonal growth on sexual reproduction.
  
  
  RESULTS
  A genomic enrichment approach was used to develop microsatellite markers. In three investigated A. kusnezoffii populations, a total of 19 microsatellite loci were successfully amplified, and 13 of these loci were polymorphic. Most of the primer pairs designed for the identified loci also amplified corresponding microsatellite loci in A. barbatum var. puberulum and A. alboviolaceum.
  
  
  CONCLUSIONS
  The identified microsatellite loci will be useful for quantifying male and female fitness in A. kusnezoffii and evaluating the effects of clonal growth on sexual reproduction.
authors.affiliation: State Key Laboratory of Earth Surface Processes and Resource Ecology, College of Life Sciences, Beijing Normal University, Beijing 100875, People's Republic of China; Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, College of Life Sciences, Beijing Normal University, Beijing 100875, People's Republic of China.
authors.forename: Xing-Yue
authors.initials: XY
authors.lastname: Ge
authors.affiliation: State Key Laboratory of Earth Surface Processes and Resource Ecology, College of Life Sciences, Beijing Normal University, Beijing 100875, People's Republic of China; Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, College of Life Sciences, Beijing Normal University, Beijing 100875, People's Republic of China.
authors.forename: Hao
authors.initials: H
authors.lastname: Tian
authors.affiliation: State Key Laboratory of Earth Surface Processes and Resource Ecology, College of Life Sciences, Beijing Normal University, Beijing 100875, People's Republic of China; Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, College of Life Sciences, Beijing Normal University, Beijing 100875, People's Republic of China.
authors.forename: Wan-Jin
authors.initials: WJ
authors.lastname: Liao
keywords: Aconitum kusnezoffii; Ranunculaceae; clonal growth; genomic enrichment cloning; microsatellite; reproductive success