title: Zinc Isotope Fractionation in the Hyperaccumulator Noccaea caerulescens and the Nonaccumulating Plant Thlaspi arvense at Low and High Zn Supply.
abstract: On the basis of our previous field survey, we postulate that the pattern and degree of zinc (Zn) isotope fractionation in the Zn hyperaccumulator Noccaea caerulescens (J. & C. Presl) F. K. Mey may reflect a relationship between Zn bioavailability and plant uptake strategies. Here, we investigated Zn isotope discrimination during Zn uptake and translocation in N. caerulescens and in a nonaccumulator Thlaspi arvense L. with a contrasting Zn accumulation ability in response to low (Zn-L) and high (Zn-H) Zn supplies. The average isotope fractionations of the N. caerulescens plant as a whole, relative to solution (Δ(66)Znplant-solution), were -0.06 and -0.12‰ at Zn-L-C and Zn-H-C, respectively, indicative of the predominance of a high-affinity (e.g., ZIP transporter proteins) transport across the root cell membrane. For T. arvense, plants were more enriched in light isotopes under Zn-H-A (Δ(66)Znplant-solution = -0.26‰) than under Zn-L-A and N. caerulescens plants, implying that a low-affinity (e.g., ion channel) transport might begin to function in the nonaccumulating plants when external Zn supply increases. Within the root tissues of both species, the apoplast fractions retained up to 30% of Zn mass under Zn-H. Moreover, the highest δ(66)Zn (0.75‰-0.86‰) was found in tightly bound apoplastic Zn, pointing to the strong sequestration in roots (e.g., binding to high-affinity ligands/precipitation with phosphate) when plants suffer from high Zn stress. During translocation, the magnitude of isotope fractionation was significantly greater at Zn-H (Δ(66)Znroot-shoot = 0.79‰) than at Zn-L, indicating that fractionation mechanisms associated with root-shoot translocation might be identical to the two plant species. Hence, we clearly demonstrated that Zn isotope fractionation could provide insight into the internal sequestration mechanisms of roots when plants respond to low and high Zn supplies.
authors.affiliation: School of Environmental Science and Engineering, Sun Yat-sen University , Guangzhou 510275, P. R. China.|Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Sun Yat-sen University , Guangzhou 510275, P. R. China.
authors.forename: Ye-Tao
authors.initials: YT
authors.lastname: Tang
authors.affiliation: CRPG-CNRS, Université de Lorraine (UMR 7358N) , 15 rue Notre-Dame-des-Pauvres BP 20, 54501 Vandoeuvre lès Nancy, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Cloquet
authors.affiliation: School of Environmental Science and Engineering, Sun Yat-sen University , Guangzhou 510275, P. R. China.
authors.forename: Teng-Hao-Bo
authors.initials: TH
authors.lastname: Deng
authors.affiliation: Laboratoire Sols et Environnement, INRA-Université de Lorraine , 2 avenue de la Forêt de Haye, TSA 40602, F-54518 Vandoeuvre-lès-Nancy Cédex, France.
authors.forename: Thibault
authors.initials: T
authors.lastname: Sterckeman
authors.affiliation: Laboratoire Sols et Environnement, INRA-Université de Lorraine , 2 avenue de la Forêt de Haye, TSA 40602, F-54518 Vandoeuvre-lès-Nancy Cédex, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Echevarria
authors.affiliation: School of Environmental Science and Engineering, Sun Yat-sen University , Guangzhou 510275, P. R. China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Yang
authors.affiliation: Laboratoire Sols et Environnement, INRA-Université de Lorraine , 2 avenue de la Forêt de Haye, TSA 40602, F-54518 Vandoeuvre-lès-Nancy Cédex, France.
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Morel
authors.affiliation: School of Environmental Science and Engineering, Sun Yat-sen University , Guangzhou 510275, P. R. China.|Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Sun Yat-sen University , Guangzhou 510275, P. R. China.
authors.forename: Rong-Liang
authors.initials: RL
authors.lastname: Qiu
mesh_terms: D019607:Brassicaceae / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D031227:Thlaspi / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism*; D015033:Zinc Isotopes / Q000378:metabolism
chemical_list: D015033:Zinc Isotopes; D015032:Zinc