title: Wheat CBL-interacting protein kinase 25 negatively regulates salt tolerance in transgenic wheat.
abstract: CBL-interacting protein kinases are involved in plant responses to abiotic stresses, including salt stress. However, the negative regulating mechanism of this gene family in response to salinity is less reported. In this study, we evaluated the role of TaCIPK25 in regulating salt response in wheat. Under conditions of high salinity, TaCIPK25 expression was markedly down-regulated in roots. Overexpression of TaCIPK25 resulted in hypersensitivity to Na(+) and superfluous accumulation of Na(+) in transgenic wheat lines. TaCIPK25 expression did not decline in transgenic wheat and remained at an even higher level than that in wild-type wheat controls under high-salinity treatment. Furthermore, transmembrane Na(+)/H(+) exchange was impaired in the root cells of transgenic wheat. These results suggested that TaCIPK25 negatively regulated salt response in wheat. Additionally, yeast-one-hybrid, β-glucuronidase activity and DNA-protein-interaction-enzyme-linked-immunosorbent assays showed that the transcription factor TaWRKY9 bound W-box in the TaCIPK25 promoter region. Quantitative real-time polymerase chain reaction assays showed concomitantly inverted expression patterns of TaCIPK25 and TaWRKY9 in wheat roots under salt treatment, ABA application and inhibition of endogenous ABA condition. Overall, based on our results, in a salt stress condition, the negative salt response in wheat involved TaCIPK25 with the expression regulated by TaWRKY9.
authors.affiliation: The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, The Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science &Technology, Wuhan 430074, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Jin
authors.affiliation: The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, The Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science &Technology, Wuhan 430074, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.affiliation: The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, The Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science &Technology, Wuhan 430074, China.
authors.forename: Xiatian
authors.initials: X
authors.lastname: Wang
authors.affiliation: The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, The Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science &Technology, Wuhan 430074, China.
authors.forename: Peipei
authors.initials: P
authors.lastname: Su
authors.affiliation: The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, The Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science &Technology, Wuhan 430074, China.
authors.forename: Jingfei
authors.initials: J
authors.lastname: Ma
authors.affiliation: The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, The Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science &Technology, Wuhan 430074, China.
authors.forename: Guangyuan
authors.initials: G
authors.lastname: He
authors.affiliation: The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, The Key Laboratory of Molecular Biophysics of Chinese Ministry of Education, College of Life Science and Technology, Huazhong University of Science &Technology, Wuhan 430074, China.
authors.forename: Guangxiao
authors.initials: G
authors.lastname: Yang
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D001483:Base Sequence; D015536:Down-Regulation / Q000187:drug effects*; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D011728:Pyridones / Q000494:pharmacology; D060888:Real-Time Polymerase Chain Reaction; D055049:Salt Tolerance / Q000187:drug effects; D016415:Sequence Alignment; D012964:Sodium / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*; D017923:Sodium-Hydrogen Exchangers / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D014908:Triticum / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D010940:Plant Proteins; D011728:Pyridones; D017923:Sodium-Hydrogen Exchangers; D014157:Transcription Factors; C013351:fluridone; D012965:Sodium Chloride; D000040:Abscisic Acid; D012964:Sodium; D011494:Protein Kinases