title: Natural variation of root exudates in Arabidopsis thaliana-linking metabolomic and genomic data.
abstract: Many metabolomics studies focus on aboveground parts of the plant, while metabolism within roots and the chemical composition of the rhizosphere, as influenced by exudation, are not deeply investigated. In this study, we analysed exudate metabolic patterns of Arabidopsis thaliana and their variation in genetically diverse accessions. For this project, we used the 19 parental accessions of the Arabidopsis MAGIC collection. Plants were grown in a hydroponic system, their exudates were harvested before bolting and subjected to UPLC/ESI-QTOF-MS analysis. Metabolite profiles were analysed together with the genome sequence information. Our study uncovered distinct metabolite profiles for root exudates of the 19 accessions. Hierarchical clustering revealed similarities in the exudate metabolite profiles, which were partly reflected by the genetic distances. An association of metabolite absence with nonsense mutations was detected for the biosynthetic pathways of an indolic glucosinolate hydrolysis product, a hydroxycinnamic acid amine and a flavonoid triglycoside. Consequently, a direct link between metabolic phenotype and genotype was detected without using segregating populations. Moreover, genomics can help to identify biosynthetic enzymes in metabolomics experiments. Our study elucidates the chemical composition of the rhizosphere and its natural variation in A. thaliana, which is important for the attraction and shaping of microbial communities.
authors.affiliation: Leibniz Institute of Plant Biochemistry, Department of Stress and Developmental Biology, Weinberg 3, 06120 Halle (Saale), Germany.
authors.forename: Susann
authors.initials: S
authors.lastname: Mönchgesang
authors.affiliation: Leibniz Institute of Plant Biochemistry, Department of Stress and Developmental Biology, Weinberg 3, 06120 Halle (Saale), Germany.
authors.forename: Nadine
authors.initials: N
authors.lastname: Strehmel
authors.affiliation: Leibniz Institute of Plant Biochemistry, Department of Stress and Developmental Biology, Weinberg 3, 06120 Halle (Saale), Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Schmidt
authors.affiliation: Leibniz Institute of Plant Biochemistry, Department of Stress and Developmental Biology, Weinberg 3, 06120 Halle (Saale), Germany.
authors.forename: Lore
authors.initials: L
authors.lastname: Westphal
authors.affiliation: Leibniz Institute of Plant Biochemistry, Department of Stress and Developmental Biology, Weinberg 3, 06120 Halle (Saale), Germany.
authors.forename: Franziska
authors.initials: F
authors.lastname: Taruttis
authors.affiliation: Leibniz Institute of Plant Biochemistry, Department of Stress and Developmental Biology, Weinberg 3, 06120 Halle (Saale), Germany.
authors.forename: Erik
authors.initials: E
authors.lastname: Müller
authors.affiliation: Leibniz Institute of Plant Biochemistry, Department of Stress and Developmental Biology, Weinberg 3, 06120 Halle (Saale), Germany.
authors.forename: Siska
authors.initials: S
authors.lastname: Herklotz
authors.affiliation: Leibniz Institute of Plant Biochemistry, Department of Stress and Developmental Biology, Weinberg 3, 06120 Halle (Saale), Germany.
authors.forename: Steffen
authors.initials: S
authors.lastname: Neumann
authors.affiliation: Leibniz Institute of Plant Biochemistry, Department of Stress and Developmental Biology, Weinberg 3, 06120 Halle (Saale), Germany.
authors.forename: Dierk
authors.initials: D
authors.lastname: Scheel
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D016000:Cluster Analysis; D003373:Coumaric Acids / Q000737:chemistry; D014644:Genetic Variation; D023281:Genomics; D005961:Glucosinolates / Q000378:metabolism; D006868:Hydrolysis; D055442:Metabolome; D055432:Metabolomics; D053147:Plant Exudates / Q000032:analysis*; D018517:Plant Roots / Q000378:metabolism; D058441:Rhizosphere; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D003373:Coumaric Acids; D005961:Glucosinolates; D053147:Plant Exudates