title: Dermal delivery of doxorubicin-loaded solid lipid nanoparticles for the treatment of skin cancer.
abstract: OBJECTIVE
  Dermal delivery of Doxorubicin (Dox) would be an ideal way in maximising drug efficiency against skin cancer accompanying with minimising side effects. We investigated the potential of Dox-loaded Solid lipid nanoparticles (SLNs) for topical delivery against skin cancer.
  
  
  METHODS
  In vitro and in vivo cytotoxicity of optimised formulation were evaluated on murine melanoma (B16F10) cells by MTT assay and melanoma induced Balb/C mice, respectively. Animal study followed by histological analysis.
  
  
  RESULTS
  Optimised formulation showed mean particle size and encapsulation efficiency (EE) of 92 nm and 86% w/w (0.86% w/w value of encapsulated Dox in the lipid matrix), respectively. FTIR experiment confirmed drug-lipid interaction interpreting the observed high EE value for Dox. In vitro and in vivo results indicated the superiority of cytotoxic performance of Dox-loaded SLN compared to Dox solution.
  
  
  CONCLUSIONS
  Our findings may open the possibilities for the topical delivery of Dox to the skin cancerous tissues.
authors.affiliation: a Biotechnology Research Center and Faculty of Pharmacy , Tabriz University of Medical Sciences , Tabriz , Iran ;
authors.forename: Ailar
authors.initials: A
authors.lastname: Tupal
authors.affiliation: b Research Center for Pharmaceutical Nanotechnology and Students' Research Committee , Tabriz University of Medical Sciences , Tabriz , Iran ;
authors.forename: Mehdi
authors.initials: M
authors.lastname: Sabzichi
authors.affiliation: c Department of Biochemistry , School of Medicine, Shiraz University of Medical Sciences , Iran ;
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Ramezani
authors.affiliation: d Department of Oral & Maxillofacial Pathology, Faculty of Dentistry , Tabriz University of Medical Science , Tabriz , Iran ;
authors.forename: Maryam
authors.initials: M
authors.lastname: Kouhsoltani
authors.affiliation: e Drug Applied Research Center , Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Hamed
authors.initials: H
authors.lastname: Hamishehkar
mesh_terms: D000287:Administration, Topical; D000818:Animals; D002460:Cell Line; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D008055:Lipids / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D008545:Melanoma / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000737:chemistry*; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D012878:Skin Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology
chemical_list: D008055:Lipids; D004317:Doxorubicin
keywords: SLN; Solid lipid nanoparticles; doxorubicin; melanoma; topical delivery