title: The effects of genistein supplementation on fructose induced insulin resistance, oxidative stress and inflammation.
abstract: OBJECTIVE
  This experimental study was designed to investigate the effects of 10weeks genistein administration on oxidative stress and inflammation in serum and liver of rats fed with fructose.
  
  
  METHODS
  6-8weeks old, 40 male Sprague-Dawley rats were included. Group 1 (control) was fed with standard chow food and 100μl/kg/day/rat dimethyl sulfoxide (DMSO) administered subcutaneously; group 2 (genistein) with standard chow food and 0.25mg/kg/day/rat genistein; group 3 (fructose) with standard chow food and drinking water 20% fructose, group 4 (fructose+genistein) with standard chow food, drinking water with 20% fructose and 0.25mg/kg/day/rat genistein. TNF-α, IL-6, visfatin as inflammatory markers and 8-isoprostane as a oxidative stress marker were measured by ELISA, glucose, triglyceride, total cholesterol, LDL-cholesterol and HDL-cholesterol by enzymatic colorimetric method, AST and ALT by kinetic UV method.
  
  
  RESULTS
  Significantly high 8-isoprostane levels in serum (p<0.001) and liver (p<0.05) in group 3 compared to control group indicate that presence of oxidative stress. Significantly high TNF-α and IL-6 levels in serum (p<0.05) and liver (p<0.01) and visfatin levels in serum (p<0.001) of group 3 indicate inflammation accompanying insulin resistance and oxidative stress. Genistein administration to fructose group causes a significant decrease in HOMA-IR (p<0.001) and LDLC (p<0.05) level. Significantly lower serum 8-isoprostane (p<0.01) level indicates the antioxidant effect of genistein and significantly lower liver TNF-α (p<0.01), serum, liver IL-6(p<0.01) and serum visfatin (p<0.01) levels reflect the antiinflammatory effects of genistein.
  
  
  CONCLUSIONS
  Genistein administration to rats fed with fructose causes an ameliorating effect on HOMA-IR values and lipid status markers in addition to its antioxidant and antiinflammatory effects.
authors.affiliation: Koc University Hospital, Clinical Laboratory, Istanbul, Turkey.
authors.forename: Said
authors.initials: S
authors.lastname: Incir
authors.affiliation: Cerrahpasa Medical Faculty, Department of Biochemistry, Istanbul, Turkey. Electronic address: mrtbola@yahoo.com.
authors.forename: I Murat
authors.initials: IM
authors.lastname: Bolayirli
authors.affiliation: Experimental Animal Research Center, Mehmet Akif State Hospital, Halkalı, Istanbul, Turkey.
authors.forename: Oznur
authors.initials: O
authors.lastname: Inan
authors.affiliation: Medical Faculty of Bezmialem Vakif University, Department of Histology and Embryology, Istanbul, Turkey.
authors.forename: M Serif
authors.initials: MS
authors.lastname: Aydın
authors.affiliation: Maslak Acibadem Hospital, Department of General Surgery, Istanbul, Turkey.
authors.forename: I Ahmet
authors.initials: IA
authors.lastname: Bilgin
authors.affiliation: Marmara University, School of Medicine, Intensive Care Unit, Turkey.
authors.forename: Ismet
authors.initials: I
authors.lastname: Sayan
authors.affiliation: Medical Faculty of Bezmialem Vakif University, Department of Histology and Embryology, Istanbul, Turkey.
authors.forename: Mukaddes
authors.initials: M
authors.lastname: Esrefoglu
authors.affiliation: Cerrahpasa Medical Faculty, Department of Biochemistry, Istanbul, Turkey.
authors.forename: Arzu
authors.initials: A
authors.lastname: Seven
mesh_terms: D000818:Animals; D005632:Fructose / Q000494:pharmacology*; D019833:Genistein / Q000494:pharmacology*; D007249:Inflammation / Q000517:prevention & control*; D007333:Insulin Resistance; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D005632:Fructose; D019833:Genistein
keywords: 8-isoprostane; Fructose; Genistein; IL-6; Inflammation; Insulin resistance; Oxidative stress; TNF-α; Visfatin