title: Serum microRNA expression profile as a diagnostic panel for gastric cancer.
abstract: OBJECTIVE
  Previously, we identified six miRNAs that are differentially expressed in colorectal cancer compared with healthy controls. Here, we tested them in gastric cancer GC.
  
  
  METHODS
  We performed quantitative RT-PCR on serum samples from 92 patients with gastric cancer and 89 controls for the six miRNAs, and analyzed their risk scores to evaluate the diagnostic value of the serum miRNA profiling system.
  
  
  RESULTS
  After a two-phase selection and validation process, five miRNAs were found to significantly differ in expression between gastric cancer samples and control samples, including miR-21, miR-31, miR-92a, miR-181b, and miR-203. Risk score analysis showed that this miRNA panel could distinguish gastric cancer cases from controls with high sensitivity and specificity. Under receiver operating characteristic curves, areas under the curve for tumor identification were 0.933 (95% confidence interval [CI]: 0.86-1.007) for the training set and 0.919 (95% CI: 0.863-0.975) for the validation set-markedly higher than those of carcinoembryonic antigen (0.624) and carbohydrate antigen 19-9 (0.603).
  
  
  CONCLUSIONS
  The signature of these five miRNAs is a novel and noninvasive biomarker for gastric cancer, and could facilitate and simplify its diagnosis.
authors.affiliation: Department of Etiology and Carcinogenesis, State Key Laboratory of Molecular Oncology, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China Beijing Key Laboratory for Carcinogenesis and Cancer Prevention, Beijing, China.
authors.forename: Shengkai
authors.initials: S
authors.lastname: Huang
authors.affiliation: Department of Clinical Laboratory, China Meitan General Hospital, Beijing, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.affiliation: Molecular Oncology Laboratory, Cancer Hospital, Affiliated Hospital of Zunyi Medical College, Zunyi, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Luo
authors.affiliation: Department of Etiology and Carcinogenesis, State Key Laboratory of Molecular Oncology, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China Beijing Key Laboratory for Carcinogenesis and Cancer Prevention, Beijing, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Department of Clinical Laboratory, China Meitan General Hospital, Beijing, China.
authors.forename: Limin
authors.initials: L
authors.lastname: Zheng
authors.affiliation: The Institute of Clinical Pharmacology of Chinese PLA General Hospital, Beijing, China.
authors.forename: Xianzhe
authors.initials: X
authors.lastname: Dong
authors.affiliation: The Institute of Clinical Pharmacology of Chinese PLA General Hospital, Beijing, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Clinical Laboratory, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
authors.forename: Yiqun
authors.initials: Y
authors.lastname: Che
authors.affiliation: The Institute of Clinical Pharmacology of Chinese PLA General Hospital, Beijing, China huangpumc@163.com qijun5610@126.com liuping301@126.com.
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.affiliation: Department of Clinical Laboratory, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China huangpumc@163.com qijun5610@126.com liuping301@126.com.
authors.forename: Jun
authors.initials: J
authors.lastname: Qi
authors.affiliation: Department of Etiology and Carcinogenesis, State Key Laboratory of Molecular Oncology, Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China Beijing Key Laboratory for Carcinogenesis and Cancer Prevention, Beijing, China huangpumc@163.com qijun5610@126.com liuping301@126.com.
authors.forename: Changzhi
authors.initials: C
authors.lastname: Huang
mesh_terms: D000368:Aged; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000097:blood; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000097:blood* / Q000235:genetics / Q000378:metabolism; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics / Q000378:metabolism; D008875:Middle Aged; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013274:Stomach Neoplasms / Q000097:blood / Q000175:diagnosis*
chemical_list: D015295:Antigens, Tumor-Associated, Carbohydrate; D014408:Biomarkers, Tumor; D035683:MicroRNAs; C578959:carbohydrate antigen 199, human
keywords: ROC curve; diagnosis; gastric cancer; serum microRNA