title: Coenzyme Activity of NAD Analogs for 3-Isopropylmalate Dehydrogenase from Thermus thermophilus HB8.
abstract: In order to elucidate the enzyme-substrate-cofactor interaction in 3-isopropylmalate dehydrogenase, the coenzyme activity of NAD analogs which have a 3-substituted pyridine ring was examined. Analogs 3-5 showed diminished k cat values compared with those of NAD(+), whereas thiocarboxamide 2 was almost as equally active as NAD(+). This suggests that the NH2 functionality of NAD(+) is more important for the catalysis of IPMDH than a carbonyl group.
authors.affiliation: a Department of Chemistry, Tokyo Institute of Technology.
authors.forename: A
authors.initials: A
authors.lastname: Chiba
authors.affiliation: b Department of Chemistry and Materials Science, Tokyo Institute of Technology.
authors.forename: T
authors.initials: T
authors.lastname: Eguchi
authors.affiliation: c School of Life Science, Tokyo University of Pharmacy and Life Science.
authors.forename: T
authors.initials: T
authors.lastname: Oshima
authors.affiliation: a Department of Chemistry, Tokyo Institute of Technology.
authors.forename: K
authors.initials: K
authors.lastname: Kakinuma
keywords: 3-isopropylmalate dehydrogenase; NAD analog; Thermus thermophilus HB8; coenzyme activity