title: A high-sensitivity HPLC assay for measuring intracellular Na(+) and K(+) and its application to Plasmodium falciparum infected erythrocytes.
abstract: The measurement of intracellular ion concentrations, and the screening of chemical agents to identify molecules targeting ion transport, has traditionally involved low-throughput techniques. Here we present a novel HPLC method that allows the rapid, high-sensitivity measurement of cell Na(+) and K(+) content, demonstrating its utility by monitoring the ionic changes induced in the intracellular malaria parasite by the new spiroindolone antimalarial KAE609.
authors.affiliation: Research School of Biology, Australian National University, Canberra, ACT 2601, Australia.|Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, UK.|Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Markus
authors.initials: M
authors.lastname: Winterberg
authors.affiliation: Research School of Biology, Australian National University, Canberra, ACT 2601, Australia.
authors.forename: Kiaran
authors.initials: K
authors.lastname: Kirk
mesh_terms: D000962:Antimalarials / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004912:Erythrocytes / Q000378:metabolism* / Q000469:parasitology*; D007211:Indoles / Q000494:pharmacology; D017136:Ion Transport / Q000187:drug effects / Q000502:physiology; D016778:Malaria, Falciparum / Q000188:drug therapy / Q000378:metabolism / Q000469:parasitology; D010963:Plasmodium falciparum / Q000187:drug effects / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*; D013141:Spiro Compounds / Q000494:pharmacology
chemical_list: D000962:Antimalarials; D007211:Indoles; C552304:NITD 609; D013141:Spiro Compounds; D012964:Sodium; D011188:Potassium