title: Effect of TRPV4-p38 MAPK Pathway on Neuropathic Pain in Rats with Chronic Compression of the Dorsal Root Ganglion.
abstract: The aim of this study was to investigate the relationships among TRPV4, p38, and neuropathic pain in a rat model of chronic compression of the dorsal root ganglion. Mechanical allodynia appeared after CCD surgery, enhanced via the intrathecal injection of 4α-phorbol 12,13-didecanoate (4α-PDD, an agonist of TRPV4) and anisomycin (an agonist of p38), but was suppressed by Ruthenium Red (RR, an inhibitor of TRPV4) and SB203580 (an inhibitor of p38). The protein expressions of p38 and P-p38 were upregulated by 4α-PDD and anisomycin injection but reduced by RR and SB203580. Moreover, TRPV4 was upregulated by 4α-PDD and SB203580 and downregulated by RR and anisomycin. In DRG tissues, the numbers of TRPV4- or p38-positive small neurons were significantly changed in CCD rats, increased by the agonists, and decreased by the inhibitors. The amplitudes of ectopic discharges were increased by 4α-PDD and anisomycin but decreased by RR and SB203580. Collectively, these results support the link between TRPV4 and p38 and their intermediary role for neuropathic pain in rats with chronic compression of the dorsal root ganglion.
authors.affiliation: Department of Physical Medicine and Rehabilitation, Qilu Hospital of Shandong University, No. 107 Wenhuaxi Road, Jinan, Shandong 250012, China.
authors.forename: Yu-Juan
authors.initials: YJ
authors.lastname: Qu
authors.affiliation: Department of Physical Medicine and Rehabilitation, Qilu Hospital of Shandong University, No. 107 Wenhuaxi Road, Jinan, Shandong 250012, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Physical Medicine and Rehabilitation, Jinan Military General Hospital, No. 25, Shifan Road, Jinan, Shandong 250031, China.
authors.forename: Zhen-Zhen
authors.initials: ZZ
authors.lastname: Fan
authors.affiliation: Department of Physical Medicine and Rehabilitation, Qilu Hospital of Shandong University, No. 107 Wenhuaxi Road, Jinan, Shandong 250012, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Huai
authors.affiliation: Department of Physical Medicine and Rehabilitation, Qilu Hospital of Shandong University, No. 107 Wenhuaxi Road, Jinan, Shandong 250012, China.
authors.forename: Yong-Bo
authors.initials: YB
authors.lastname: Teng
authors.affiliation: Department of Physical Medicine and Rehabilitation, Qilu Hospital of Shandong University, No. 107 Wenhuaxi Road, Jinan, Shandong 250012, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Physical Medicine and Rehabilitation, Qilu Hospital of Shandong University, No. 107 Wenhuaxi Road, Jinan, Shandong 250012, China.
authors.forename: Shou-Wei
authors.initials: SW
authors.lastname: Yue
mesh_terms: D000818:Animals; D002908:Chronic Disease; D055724:Electrophysiological Phenomena / Q000187:drug effects; D005727:Ganglia, Spinal / Q000473:pathology; D006930:Hyperalgesia / Q000473:pathology / Q000503:physiopathology; D007278:Injections, Spinal; D008297:Male; D009408:Nerve Compression Syndromes / Q000378:metabolism* / Q000473:pathology; D009437:Neuralgia / Q000378:metabolism* / Q000473:pathology; D017288:Pain Threshold; D010766:Phosphorylation; D017208:Rats, Wistar; D015398:Signal Transduction; D050916:TRPV Cation Channels / Q000037:antagonists & inhibitors / Q000378:metabolism*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: D050916:TRPV Cation Channels; C486577:Trpv4 protein, rat; D048051:p38 Mitogen-Activated Protein Kinases