title: Nanometer-grooved topography stimulates trabecular bone regeneration around a concave implant in a rat femoral medulla model.
abstract: In the present study, a method was developed to reproduce two nanogrooved patterns (groove width/ridge width/depth: 150/150/50 nm and 200/800/70 nm) into cylindrical epoxy resin implants, which were subsequently coated with 20 nm of titanium. Also, implants with a conventional surface roughness (Rq=1.6 μm) were produced. After cytocompatibility analysis of the produced surfaces, implants were installed into the femoral condyle of rats for 4 and 8 weeks. The histomorphometrical analysis of bone volume in a 100 μm wide zone close to the implant surface showed that only for the 200/800 grooves the amount of bone increased significantly between 4 and 8 weeks of implantation. In addition, at the late time point only implants with the 200/800 pattern revealed a significantly higher bone volume compared to the rough controls. In conclusion, the 200/800 grooved pattern can positively influence bone volume adjacent to the implant surface, and should be evaluated and optimized in further (pre-)clinical studies.
authors.affiliation: Department of Biomaterials, Radboud University Medical Center, Nijmegen, the Netherlands.
authors.forename: Alexey
authors.initials: A
authors.lastname: Klymov
authors.affiliation: Department of Tumor Immunology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, the Netherlands.
authors.forename: Joost
authors.initials: J
authors.lastname: Te Riet
authors.affiliation: Institute for Molecules and Materials, Radboud University Nijmegen, Nijmegen, the Netherlands.
authors.forename: Peter
authors.initials: P
authors.lastname: Mulder
authors.affiliation: Mesoscale Chemical Systems Group, MESA+ Institute for Nanotechnology, University of Twente, Enschede, the Netherlands.
authors.forename: Johannes G E
authors.initials: JG
authors.lastname: Gardeniers
authors.affiliation: Department of Biomaterials, Radboud University Medical Center, Nijmegen, the Netherlands.
authors.forename: John A
authors.initials: JA
authors.lastname: Jansen
authors.affiliation: Department of Biomaterials, Radboud University Medical Center, Nijmegen, the Netherlands. Electronic address: Frank.Walboomers@radboudumc.nl.
authors.forename: X Frank
authors.initials: XF
authors.lastname: Walboomers
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D000071556:Cancellous Bone; D005269:Femur; D036103:Nanotechnology; D016348:Osseointegration; D019736:Prostheses and Implants; D051381:Rats; D012038:Regeneration; D013499:Surface Properties; D014025:Titanium
chemical_list: D014025:Titanium
keywords: Bone implants; Bone regeneration; Nanogrooves; Nanotopography; Rat femoral condyle