title: Genes regulated by potassium channel tetramerization domain containing 15 (Kctd15) in the developing neural crest.
abstract: Neural crest (NC) development is controlled precisely by a regulatory network with multiple signaling pathways and the involvement of many genes. The integration and coordination of these factors are still incompletely understood. Overexpression of Wnt3a and the BMP antagonist Chordin in animal cap cells from Xenopus blastulae induces a large number of NC specific genes. We previously suggested that Potassium Channel Tetramerization Domain containing 15 (Kctd15) regulates NC formation by affecting Wnt signaling and the activity of transcription factor AP-2. In order to advance understanding of the function of Kctd15 during NC development, we performed DNA microarray assays in explants injected with Wnt3a and Chordin, and identified genes that are affected by Kctd15 overexpression. Among the many genes identified, we chose Duf domain containing protein 1 (ddcp1), Platelet-Derived Growth Factor Receptor a (pdgfra), Complement factor properdin (cfp), Zinc Finger SWIM-Type Containing 5 (zswim5), and complement component 3 (C3) to examine their expression by whole mount in situ hybridization. Our work points to a possible role for Kctd15 in the regulation of NC formation and other steps in embryonic development.
authors.affiliation: School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, P. R. China.
authors.forename: Thomas C B
authors.initials: TC
authors.lastname: Wong
authors.forename: Martha
authors.initials: M
authors.lastname: Rebbert
authors.forename: Chengdong
authors.initials: C
authors.lastname: Wang
authors.forename: Xiongfong
authors.initials: X
authors.lastname: Chen
authors.forename: Alison
authors.initials: A
authors.lastname: Heffer
authors.forename: Valeria E
authors.initials: VE
authors.lastname: Zarelli
authors.forename: Igor B
authors.initials: IB
authors.lastname: Dawid
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
mesh_terms: D000818:Animals; D047108:Embryonic Development; D018507:Gene Expression Regulation, Developmental; D053263:Gene Regulatory Networks; D009432:Neural Crest / Q000196:embryology / Q000378:metabolism*; D024642:Potassium Channels, Voltage-Gated / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D015854:Up-Regulation; D051153:Wnt Proteins / Q000378:metabolism; D029867:Xenopus Proteins / Q000235:genetics* / Q000378:metabolism; D014982:Xenopus laevis / Q000196:embryology*
chemical_list: C552214:Kctd15 protein, Xenopus; D024642:Potassium Channels, Voltage-Gated; D051153:Wnt Proteins; D029867:Xenopus Proteins