title: Inhibition of Plant Transformation by Phytolaccoside B from Phytolacca americana Callus.
abstract: The newly established GUS expression bioassay on the callus extracts of 22 species of plants revealed that the methanol extract of Phytolacca americana callus had the most potent inhibitory activity against agrobacterial plant transformation. A triterpene glycoside phytolaccoside B was isolated from the extract as a genuine plant transformation inhibitor having neither antiagrobacterial nor phytotoxic activity. This compound is promising for use as a biochemical probe for studies on the plant transformation mechanism.
authors.affiliation: a Laboratory of Bioresources Chemistry, Faculty of Agriculture, Okayama University.
authors.forename: H
authors.initials: H
authors.lastname: Kanzaki
authors.affiliation: a Laboratory of Bioresources Chemistry, Faculty of Agriculture, Okayama University.
authors.forename: T
authors.initials: T
authors.lastname: Kagemori
authors.affiliation: a Laboratory of Bioresources Chemistry, Faculty of Agriculture, Okayama University.
authors.forename: Y
authors.initials: Y
authors.lastname: Yamachika
authors.affiliation: a Laboratory of Bioresources Chemistry, Faculty of Agriculture, Okayama University.
authors.forename: T
authors.initials: T
authors.lastname: Nitoda
authors.affiliation: a Laboratory of Bioresources Chemistry, Faculty of Agriculture, Okayama University.
authors.forename: K
authors.initials: K
authors.lastname: Kawazu
keywords: Ageratum conyzoides; Agrobacterium tumefaciens; GUS expression assay; Phytolacca americana