title: Determination of Catechin and Epicatechin Content in Chocolates by High-Performance Liquid Chromatography.
abstract: A simple and sensitive reversed phase high-performance liquid chromatographic (HPLC) method has been developed for the determination of catechin and epicatechin in cocoa powder and chocolates. The separation was achieved on a reversed phase C 18 column (TARGA) 5 μm by gradient elution with a flow rate of 1.0 mL/minute with an operating temperature of 30°C and detection with a UV-Visible detector was at 280 nm. The method was validated for linearity, precision, intra- and interday precision, and accuracy. The developed method is successfully applied for the determination of catechin and epicatechin content in chocolates. The Godiva brand chocolate contains high concentration of epicatechin.
authors.affiliation: Natsol Laboratories Private Limited, 2nd Floor, Research & Development Building, Ramky Commercial Hub, Jawaharlal Nehru Pharma City, Visakhapatnam 531 019, India.
authors.forename: Raju V S S
authors.initials: RV
authors.lastname: Gottumukkala
authors.affiliation: Natsol Laboratories Private Limited, 2nd Floor, Research & Development Building, Ramky Commercial Hub, Jawaharlal Nehru Pharma City, Visakhapatnam 531 019, India.
authors.forename: Nareshraju
authors.initials: N
authors.lastname: Nadimpalli
authors.affiliation: Natsol Laboratories Private Limited, 2nd Floor, Research & Development Building, Ramky Commercial Hub, Jawaharlal Nehru Pharma City, Visakhapatnam 531 019, India.
authors.forename: Kannababu
authors.initials: K
authors.lastname: Sukala
authors.affiliation: Natsol Laboratories Private Limited, 2nd Floor, Research & Development Building, Ramky Commercial Hub, Jawaharlal Nehru Pharma City, Visakhapatnam 531 019, India.
authors.forename: Gottumukkala V
authors.initials: GV
authors.lastname: Subbaraju