title: Analysis of monosomy-3 in immunomagnetically isolated circulating melanoma cells in uveal melanoma patients.
abstract: Monosomy-3 in primary uveal melanoma (UM) is associated with a high risk of metastasis and mortality. Although circulating melanoma cells (CMC) can be found in most UM patients, only approximately 50% of the patients develop metastases. We utilized a novel immuno-FISH assay to detect chromosome-3 in intact CMC isolated by dual immunomagnetic enrichment. Circulating melanoma cells were detected in 91% of the patients (n = 44) with primary non-metastatic UM, of which 58% were positive for monosomy-3. The monosomy-3 status of CMC corresponded to the monosomy-3 status of the primary tumor in 10 of the 11 patients where this could be tested. Monosomy-3 in the CMC was associated with an advanced tumor stage (P = 0.046) and was detected in all four patients who developed metastasis within the follow-up period of 4 yr. This non-invasive technique may enable the identification of UM patients at risk for metastasis particularly when a primary tumor specimen is unavailable.
authors.affiliation: Department of Ophthalmology, University of Lübeck, Lübeck, Germany. ayseguel.tura@uksh.de.
authors.forename: Aysegül
authors.initials: A
authors.lastname: Tura
authors.affiliation: Department of Pathology, University of Lübeck, Lübeck, Germany.
authors.forename: Hartmut
authors.initials: H
authors.lastname: Merz
authors.affiliation: Department of Ophthalmology, University of Lübeck, Lübeck, Germany.
authors.forename: Mihaela
authors.initials: M
authors.lastname: Reinsberg
authors.affiliation: Department of Ophthalmology, University of Lübeck, Lübeck, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Lüke
authors.affiliation: Department of Ophthalmology, Leiden University Medical Center, Leiden, The Netherlands.
authors.forename: Martine J
authors.initials: MJ
authors.lastname: Jager
authors.affiliation: Department of Ophthalmology, University of Lübeck, Lübeck, Germany.
authors.forename: Salvatore
authors.initials: S
authors.lastname: Grisanti
authors.affiliation: Department of Ophthalmology, University of Lübeck, Lübeck, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Lüke
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics*; D002893:Chromosomes, Human, Pair 3; D005260:Female; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D007621:Karyotyping; D008297:Male; D008545:Melanoma / Q000097:blood / Q000235:genetics* / Q000473:pathology; D008875:Middle Aged; D009006:Monosomy; D009360:Neoplastic Cells, Circulating / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured; D014604:Uveal Neoplasms / Q000097:blood / Q000235:genetics* / Q000473:pathology; D000098943:Uveal Melanoma
chemical_list: D014408:Biomarkers, Tumor
keywords: circulating melanoma cells; dual immunomagnetic enrichment; immuno-FISH; monosomy-3; uveal melanoma