title: A sensitive LC-MS/MS method for the quantification of urinary 8-iso-prostaglandin F2α (8-iso-PGF2α) including pediatric reference interval.
abstract: BACKGROUND
  Oxidative stress has been implicated in numerous diseases, including arthritis, atherosclerosis, Alzheimer's disease, cancer, diabetes, hypertension, and inflammation. 8-iso-prostaglandin F2α, a member of the F2 isoprostane family, has been well-accepted as a valuable biomarker for the assessment of oxidative stress.
  
  
  METHODS
  We report the development and validation of an ultra-sensitive LC-MS/MS assay for urinary 8-iso-PGF2α measurements in pediatric population.
  
  
  RESULTS
  The assay was linear from 0.024 to 20nmol/l (R(2)=0.99). Recoveries were above 85% and matrix effects were below 5%. The variability was determined at nmol/l concentration: the intra-day variability (%CV) ranged from 3.9% to 4.5% (n=20); and the inter-day variability ranged from 4.3% to 5.7% (n=20). The accuracy of our laboratory developed test was evaluated with a clinical reference laboratory (n=39), and a correlation coefficient of 0.9257 was observed. Reference interval were established to be <0.5ng/mg creatinine in a group of pediatric population (2months-18years, n=123).
  
  
  CONCLUSIONS
  The precision of the assay will allow for accurate assessment of oxidative stress, and is acceptable for patient testing, particularly in pediatric population.
authors.affiliation: Department of Pathology and Laboratory Medicine, Children's Hospital of Los Angeles, Los Angeles, CA 90027, United States.
authors.forename: Yi
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Department of Pathology and Laboratory Medicine, Children's Hospital of Los Angeles, Los Angeles, CA 90027, United States; Departments of Pathology, University of Southern California, Los Angeles, CA 90089, United States. Electronic address: xfu@chla.usc.edu.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Fu
authors.affiliation: Department of Pathology and Laboratory Medicine, Children's Hospital of Los Angeles, Los Angeles, CA 90027, United States; Departments of Pathology, University of Southern California, Los Angeles, CA 90089, United States.
authors.forename: Paul
authors.initials: P
authors.lastname: Pattengale
authors.affiliation: Department of Pathology and Laboratory Medicine, Children's Hospital of Los Angeles, Los Angeles, CA 90027, United States; Departments of Pathology, University of Southern California, Los Angeles, CA 90089, United States.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Dien Bard
authors.affiliation: Department of Pathology and Laboratory Medicine, Children's Hospital of Los Angeles, Los Angeles, CA 90027, United States.
authors.forename: Yan-Kang
authors.initials: YK
authors.lastname: Xu
authors.affiliation: Department of Pathology and Laboratory Medicine, Children's Hospital of Los Angeles, Los Angeles, CA 90027, United States; Departments of Pathology, University of Southern California, Los Angeles, CA 90089, United States; Pediatrics Keck School of Medicine, University of Southern California, Los Angeles, CA 90089, United States. Electronic address: mogorman@chla.usc.edu.
authors.forename: Maurice R
authors.initials: MR
authors.lastname: O'Gorman
mesh_terms: D015415:Biomarkers / Q000032:analysis; D002623:Chemistry Techniques, Analytical / Q000379:methods* / Q000592:standards; D002648:Child; D002675:Child, Preschool; D002853:Chromatography, Liquid / Q000379:methods; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000032:analysis; D006801:Humans; D007223:Infant; D018384:Oxidative Stress; D012016:Reference Values; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D015415:Biomarkers; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost
keywords: 8-iso-PGF(2α); 8-iso-Prostaglandin F(2α); Liquid chromatography-mass spectrometry; Pediatric reference interval