title: Lysobacter humi sp. nov., a bacterium isolated from rice field.
abstract: A novel bacterial strain THG-PC4Twas isolated from soil sample of rice field and was characterized by using a polyphasic approach. Cells were Gram-negative, bright yellow colored, nonmotile and rod shaped. The strain was aerobic and catalase positive, optimum growth temperature, and pH were 28 °C and 7, respectively. On the basis of 16S rRNA gene sequence analysis, strain THG-PC4Tbelongs to the genus Lysobacter and is most closely related to Lysobacter fragariae KCTC 42236T, followed by Lysobacter oryzae KCTC 22249T, Lysobacter tyrosinelyticus KCTC 42235T, Lysobacter terrae KACC 17646T, Lysobacter yangpyeongensis KACC 11407T, Lysobacter rhizosphaerae KCTC 42237Tand Lysobacter niabensis KACC 11587T. In DNA-DNA hybridization tests, the DNA relatedness between strain THG-PC4Tand its closest phylogenetic neighbors was below 45 %. The DNA G + C content was 66.6 mol %, and the predominant respiratory quinone was ubiquinone-8. Flexirubin-type pigments were found to be present. The major cellular fatty acids were iso-C16:0, iso-C17:1ω9c, iso-C17:0and iso-C11:03OH. The predominant polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The DNA-DNA hybridization, genotypic, chemotaxonomic and physiological data demonstrated that strain THG-PC4Trepresented a novel species within the genus Lysobacter, for which the name Lysobcater humi is proposed. The type strain is THG-PC4T(=KACC 18284T= CCTCC AB 2015292T).
authors.affiliation: Department of Oriental Medicine Biotechnology, College of Life Science, Kyung Hee University, Global Campus, 1732 Deokyoungdaero, Giheung-gu, Yongin-si, Gyeonggi-do, 446-701, Republic of Korea.
authors.forename: Shahina
authors.initials: S
authors.lastname: Akter
authors.affiliation: Graduate School of Biotechnology, Kyung Hee University Global Campus, 1732 Deokyoungdaero, Giheung-gu, Yongin-si, Gyeonggi-do, 446-701, Republic of Korea.
authors.forename: Md Amdadul
authors.initials: MA
authors.lastname: Huq
authors.affiliation: Department of Oriental Medicine Biotechnology, College of Life Science, Kyung Hee University, Global Campus, 1732 Deokyoungdaero, Giheung-gu, Yongin-si, Gyeonggi-do, 446-701, Republic of Korea. drhoo@khu.ac.kr.
authors.forename: Tae-Hoo
authors.initials: TH
authors.lastname: Yi
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D053528:Lysobacter / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D012275:Oryza / Q000382:microbiology*; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014451:Ubiquinone / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030778:ubiquinone 8
keywords: 16S rRNA; Gram-negative; Lysobacter humi; Ubiquinone-8