title: FCN2 c.772G>T polymorphism is associated with chronic adenoiditis and/or tonsillitis, but not -4 A>G and -602 G>A.
abstract: OBJECTIVE
  Ficolins are complement activating peptides that play a role in the initial host defense against infectious pathogens. In the present study, we investigated the relationship between single nucleotide polymorphisms (SNPs) in the ficolin 2 gene (FCN2) and chronic adenotonsillitis in pediatric cases.
  
  
  METHODS
  Case-control study.
  
  
  METHODS
  A total of 101 pediatric patients diagnosed with chronic adenotonsillitis and 100 healthy children were enrolled in the study. Genotypes of FCN2 promoter SNPs - 602 G>A and -4 A>G, and the exonic SNP c.772G>T were determined by light SNP assay after realtime PCR analysis using genomic DNA samples obtained from peripheral blood samples of all participants.
  
  
  RESULTS
  Of the 101 chronic tonsillitis patients, 38 were girls and 63 were boys; the mean age was 5.2 ± 2.3 years. The c.772G>T SNP frequency was significantly higher in chronic adenotonsillitis cases compared to the control group (p = 0.00); however, no significant difference was determined at positions -602 G>A or -4 A>G (p > 0.05).
  
  
  CONCLUSIONS
  The FCN2 c.772G>T genotype appears to be associated with predisposition to chronic adenotonsillitis in the pediatric age group. This nucleotide change is likely to influence the level of gene expression and contribute to the development of disease.
authors.affiliation: Department of Otolaryngology, Faculty of Medicine, Baskent University, 06490 Ankara, Turkey.
authors.forename: Alper N
authors.initials: AN
authors.lastname: Erkan
authors.affiliation: Department of Otolaryngology, Faculty of Medicine, Baskent University, 06490 Ankara, Turkey. Electronic address: isilaydgn@yahoo.com.
authors.forename: Isilay
authors.initials: I
authors.lastname: Oz
authors.affiliation: Department of Medical Genetics, Faculty of Medicine, Baskent University, 06490 Ankara, Turkey.
authors.forename: Yunus K
authors.initials: YK
authors.lastname: Terzi
authors.affiliation: Department of Otolaryngology, Faculty of Medicine, Baskent University, 06490 Ankara, Turkey.
authors.forename: Erdinc
authors.initials: E
authors.lastname: Aydin
authors.affiliation: Department of Pediatrics, Faculty of Medicine, Baskent University, 06490 Ankara, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Ozkale
authors.affiliation: Department of Otolaryngology, Faculty of Medicine, Baskent University, 06490 Ankara, Turkey.
authors.forename: Seda Turkoglu
authors.initials: ST
authors.lastname: Babakurban
authors.affiliation: Department of Otolaryngology, Faculty of Medicine, Baskent University, 06490 Ankara, Turkey.
authors.forename: Alper
authors.initials: A
authors.lastname: Koycu
authors.affiliation: Department of Medical Genetics, Faculty of Medicine, Baskent University, 06490 Ankara, Turkey.
authors.forename: Feride Iffet
authors.initials: FI
authors.lastname: Sahin
mesh_terms: D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D002908:Chronic Disease; D005091:Exons; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D037102:Lectins / Q000235:genetics*; D008297:Male; D009304:Nasopharyngitis / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D014069:Tonsillitis / Q000235:genetics*; D000097604:Ficolins
chemical_list: D037102:Lectins; D000097604:Ficolins
keywords: Chronic adenotonsillitis; Ficolin 2; Genotype; Polymorphism