title: Effect of Layer-by-Layer (LbL) Encapsulation of Nano-Emulsified Fish Oil on Their Digestibility Ex Vivo and Skin Permeability In Vitro.
abstract: Omega-3 rich fish oils are extremely labile, thus requiring control of oxidation and off flavor development. A recently proposed emulsification method, layer-by-layer (LbL) deposition, was found to be a plausible method to enhance the characteristics of bioactive ingredients, especially lipids. The present work was designed to test the possibility of enhancing the uptake and utilization of omega-3 fatty acids present in fish oil. The bioavailability of nano-emulsified fish oil was monitored in terms of intestinal absorption as well as skin permeability by using the everted intestinal sac model and Franz cell model. The skin permeability and intestinal absorption characteristics was significantly improved by LbL emulsification with lecithin/chitosan/low methoxypectin. Multilayer encapsulation along with nano-emulsification can be a useful method to deliver biologically active lipids and related components, such as fish oil. The protective effect of this tool from lipid oxidation still needs to be verified.
authors.affiliation: Department of Home Economic Education, Jeonju University, Jeonbuk 55069, Korea.
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Jung
authors.affiliation: Department of Public Health Sciences, Graduate School, Korea University, Seoul 02841, Korea.
authors.forename: Ki Bae
authors.initials: KB
authors.lastname: Hong
authors.affiliation: Department of Food Science and Biotechnology, Shin Ansan University, Gyeonggi 15435, Korea.
authors.forename: Heung Soo
authors.initials: HS
authors.lastname: Son
authors.affiliation: Department of Public Health Sciences, Graduate School, Korea University, Seoul 02841, Korea.
authors.forename: Hyung Joo
authors.initials: HJ
authors.lastname: Suh
authors.affiliation: Dongguk University Research Institute of Biotechnology & Medical Converged Science, Dongguk University, Gyeonggi 10325, Korea.
authors.forename: Yooheon
authors.initials: Y
authors.lastname: Park
keywords: Franz cell; encapsulation; everted intestine sac; fish oils; nano-emulcification