title: Impact of Repeated Tail Clip and Saphenous Vein Phlebotomy on Rats Used in Toxicology Studies.
abstract: Sampling blood for toxicokinetic (TK) evaluation in rodents is typically performed using a satellite group of animals to avoid depleting the blood volume and inducing an additional stressor in the main study animals. This practice does not allow for direct comparison of individual animal toxicity to exposure. These studies evaluated serial collection of twelve, 40-µl blood samples from each rat from either a tail clip or a saphenous vein bleed and its impact on toxicologic parameters over 4- and 14-day periods. The results show the feasibility of successfully collecting TK samples from main study animals, using either of the two techniques. Both procedures were amenable to execution by a single technician using dried blood spot sampling. Any changes observed in the primary markers of erythroid mass between the nonbled control rats and repeat sampled rats were minimal and the range of values often overlapped. This technique would improve the quality of data generated from toxicology studies by allowing a direct comparison of systemic exposure to toxicity while at the same time reducing the number of rats by obviating the need for satellite groups.
authors.affiliation: Lilly Research Laboratories, Indianapolis, Indiana, USA wickremsinhe_enaksha@lilly.com.
authors.forename: Enaksha R
authors.initials: ER
authors.lastname: Wickremsinhe
authors.affiliation: Covance Laboratories Inc., Greenfield, Indiana, USA.
authors.forename: Matthew
authors.initials: M
authors.lastname: Renninger
authors.affiliation: Covance Laboratories Inc., Greenfield, Indiana, USA.
authors.forename: April
authors.initials: A
authors.lastname: Paulman
authors.affiliation: Lilly Research Laboratories, Indianapolis, Indiana, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Pritt
authors.affiliation: Lilly Research Laboratories, Indianapolis, Indiana, USA.
authors.forename: Albert E
authors.initials: AE
authors.lastname: Schultze
mesh_terms: D000818:Animals; D001800:Blood Specimen Collection / Q000379:methods*; D005260:Female; D051381:Rats; D017207:Rats, Sprague-Dawley; D012501:Saphenous Vein / Q000601:surgery; D013525:Surgical Instruments; D013623:Tail / Q000601:surgery; D018675:Toxicity Tests / Q000379:methods*
keywords: clinical pathology data interpretation; dried blood spots; hematology; phlebotomy techniques; toxicokinetic blood samples