title: Purification of organic acids by chromatography with strong anionic resins: Investigation of uptake mechanisms.
abstract: Bio-based organic acids are promising renewable carbon sources for the chemical industry. However energy-consuming purification processes are used, like distillation or crystallization, to reach high purities required in some applications. That is why preparative chromatography was studied as an alternative separation technique. In a previous work dealing with the purification of lactic, succinic and citric acids, the Langmuir model was insufficient to explain the elution profiles obtained with a strong anionic resin. Consequently the Langmuir model was coupled with a usual ion-exchange model to take into account the retention of their conjugate bases (<2%), which are commonly neglected at low pH (<1.5). Elution simulations with both uptake mechanisms fitted very well with experimental pulse tests. Only two parameters were optimized (equilibrium constant of acid uptake and ion-exchange selectivity coefficient of conjugate base) and their value were coherent with experimental and resin suppliers' data. These results confirmed that the singular tailing and apparent delay observed with succinic and citric acids can be explained by the high affinity of succinate and citrate for resin cationic sites. The model was implemented in a preparative chromatography simulation program in order to optimize operating parameters of our pilot-scale ISMB unit (Improved Simulated Moving Bed). The comparison with experimental ISMB profiles was conclusive.
authors.affiliation: LGPM, CentraleSupélec, Université Paris-Saclay, SFR Condorcet FR CNRS 3417, Centre Européen de Biotechnologie et de Bioéconomie (CEBB), 3 rue des Rouges-Terres, 51110 Pomacle, France. Electronic address: julien.lemaire@centralesupelec.fr.
authors.forename: Julien
authors.initials: J
authors.lastname: Lemaire
authors.affiliation: LGPM, CentraleSupélec, Université Paris-Saclay, SFR Condorcet FR CNRS 3417, Centre Européen de Biotechnologie et de Bioéconomie (CEBB), 3 rue des Rouges-Terres, 51110 Pomacle, France; Eurodia Industrie, ZAC Saint Martin, Impasse Saint Martin, 84120 Pertuis, France.
authors.forename: Claire-Line
authors.initials: CL
authors.lastname: Blanc
authors.affiliation: Eurodia Industrie, ZAC Saint Martin, Impasse Saint Martin, 84120 Pertuis, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Lutin
authors.affiliation: LGPM, CentraleSupélec, Université Paris-Saclay, SFR Condorcet FR CNRS 3417, Centre Européen de Biotechnologie et de Bioéconomie (CEBB), 3 rue des Rouges-Terres, 51110 Pomacle, France.
authors.forename: Marc-André
authors.initials: MA
authors.lastname: Théoleyre
authors.affiliation: LGPM, CentraleSupélec, Université Paris-Saclay, SFR Condorcet FR CNRS 3417, Centre Européen de Biotechnologie et de Bioéconomie (CEBB), 3 rue des Rouges-Terres, 51110 Pomacle, France.
authors.forename: Moncef
authors.initials: M
authors.lastname: Stambouli
authors.affiliation: LGPM, CentraleSupélec, Université Paris-Saclay, SFR Condorcet FR CNRS 3417, Centre Européen de Biotechnologie et de Bioéconomie (CEBB), 3 rue des Rouges-Terres, 51110 Pomacle, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Pareau
mesh_terms: D000837:Anion Exchange Resins / Q000737:chemistry*; D000838:Anions / Q000737:chemistry; D002412:Cations / Q000737:chemistry; D002852:Chromatography, Ion Exchange / Q000379:methods*; D019343:Citric Acid / Q000737:chemistry / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007474:Ion Exchange; D008956:Models, Chemical; D019802:Succinic Acid / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D000837:Anion Exchange Resins; D000838:Anions; D002412:Cations; D019343:Citric Acid; D019802:Succinic Acid
keywords: Anionic resin; Chromatography; Modelling; Organic acid; Sustainable processing