title: Development of a multiplex PCR assay for rapid virulence factor profiling of extraintestinal pathogenic Escherichia coli isolated from cattle.
abstract: Virulence factor (VF) profiling is important for the control of extraintestinal pathogenic Escherichia coli (ExPEC) infection because VF prevalence is highly variable. We analyzed the VF profile of ExPEC isolated from cattle in Yamagata prefecture, Japan, 2000-2015 and developed a rapid VF profiling method using a multiplex PCR assay.
authors.affiliation: Yamagata Prefecture Central Livestock Hygiene Service Center, 736 Urushiyama, Yamagata 990-2161, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Ojima
authors.affiliation: Yamagata Prefecture Central Livestock Hygiene Service Center, 736 Urushiyama, Yamagata 990-2161, Japan.
authors.forename: Kaori
authors.initials: K
authors.lastname: Hirano
authors.affiliation: Yamagata Prefecture Central Livestock Hygiene Service Center, 736 Urushiyama, Yamagata 990-2161, Japan.
authors.forename: Kohei
authors.initials: K
authors.lastname: Honda
authors.affiliation: Division of Bacterial and Parasitic Disease, National Institute of Animal Health, National Agriculture and Food Research Organization, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan. Electronic address: kusu555@affrc.go.jp.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kusumoto
mesh_terms: D000818:Animals; D002417:Cattle / Q000382:microbiology*; D004269:DNA, Bacterial / Q000302:isolation & purification; D000072282:Extraintestinal Pathogenic Escherichia coli / Q000235:genetics / Q000302:isolation & purification*; D020869:Gene Expression Profiling; D005798:Genes, Bacterial; D007564:Japan; D060885:Multiplex Polymerase Chain Reaction; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D037521:Virulence Factors
keywords: Cattle; Extraintestinal pathogenic Escherichia coli; Multiplex PCR assay; Virulence factor profiling