title: Detection of Citrus tristeza virus by using fluorescence resonance energy transfer-based biosensor.
abstract: Due to the low titer or uneven distribution of Citrus tristeza virus (CTV) in field samples, detection of CTV by using conventional detection techniques may be difficult. Therefore, in the present work, the cadmium-telluride quantum dots (QDs) was conjugated with a specific antibody against coat protein (CP) of CTV, and the CP were immobilized on the surface of gold nanoparticles (AuNPs) to develop a specific and sensitive fluorescence resonance energy transfer (FRET)-based nanobiosensor for detecting CTV. The maximum FRET efficiency for the developed nano-biosensor was observed at 60% in AuNPs-CP/QDs-Ab ratio of 1:8.5. The designed system showed higher sensitivity and specificity over enzyme linked immunosorbent assay (ELISA) with a limit of detection of 0.13μgmL(-1) and 93% and 94% sensitivity and specificity, respectively. As designed sensor is rapid, sensitive, specific and efficient in detecting CTV, this could be envisioned for diagnostic applications, surveillance and plant certification program.
authors.affiliation: Institute of Advanced Technology (ITMA), Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. Electronic address: tahashojaee2000@yahoo.com.
authors.forename: Taha Roodbar
authors.initials: TR
authors.lastname: Shojaei
authors.affiliation: Department of Chemical and Environmental Engineering, Faculty of Engineering, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. Electronic address: asalleh@upm.edu.my.
authors.forename: Mohamad Amran Mohd
authors.initials: MA
authors.lastname: Salleh
authors.affiliation: Department of Plant Protection, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Kamaruzaman
authors.initials: K
authors.lastname: Sijam
authors.affiliation: Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Raha Abdul
authors.initials: RA
authors.lastname: Rahim
authors.affiliation: Research and Development Department, Nanozino, 16536-43181 Tehran, Iran.
authors.forename: Afshin
authors.initials: A
authors.lastname: Mohsenifar
authors.affiliation: Department of Plant Viruses, Iranian Institute of Plant Protection, Tehran, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Safarnejad
authors.affiliation: Nanosystems Research Team (NRTeam), Microbial Biotechnology and Biosafety Dept., Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran.
authors.forename: Meisam
authors.initials: M
authors.lastname: Tabatabaei
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods; D019187:Cadmium Compounds / Q000737:chemistry; D002957:Citrus / Q000821:virology*; D017790:Closterovirus / Q000302:isolation & purification*; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D006046:Gold / Q000737:chemistry; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D010935:Plant Diseases / Q000821:virology*; D045663:Quantum Dots / Q000737:chemistry; D013691:Tellurium / Q000737:chemistry
chemical_list: D055910:Antibodies, Immobilized; D019187:Cadmium Compounds; D006046:Gold; D013691:Tellurium; C028337:cadmium telluride
keywords: Bioconjugation; Cadmium-telluride quantum dots; Fluorometric immunoassay; Gold nanoparticles; Nanobiosensor; Quenching