title: Fully automated synthesis of [(18) F]fluoro-dihydrotestosterone ([(18) F]FDHT) using the FlexLab module.
abstract: Imaging of androgen receptor expression in prostate cancer using F-18 FDHT is becoming increasingly popular. With the radiolabelling precursor now commercially available, developing a fully automated synthesis of [(18) F] FDHT is important. We have fully automated the synthesis of F-18 FDHT using the iPhase FlexLab module using only commercially available components. Total synthesis time was 90 min, radiochemical yields were 25-33% (n = 11). Radiochemical purity of the final formulation was > 99% and specific activity was > 18.5 GBq/µmol for all batches. This method can be up-scaled as desired, thus making it possible to study multiple patients in a day. Furthermore, our procedure uses 4 mg of precursor only and is therefore cost-effective. The synthesis has now been validated at Austin Health and is currently used for [(18) F]FDHT studies in patients. We believe that this method can easily adapted by other modules to further widen the availability of [(18) F]FDHT.
authors.affiliation: Department of Molecular Imaging and Therapy, Austin Health, Heidelberg, Australia.|Olivia Newton-John Cancer Research Institute, Heidelberg, Australia.|School of Medicine, Dentistry and Health Sciences, The University of Melbourne, Parkville, Australia.|School of Cancer Medicine, LaTrobe University, Australia.
authors.forename: Uwe
authors.initials: U
authors.lastname: Ackermann
authors.affiliation: Radiochemistry & Molecular Imaging Probe Core, Memorial Sloan Kettering Cancer Center, New York, NY, USA.
authors.forename: Jason S
authors.initials: JS
authors.lastname: Lewis
authors.affiliation: Department of Molecular Imaging and Therapy, Austin Health, Heidelberg, Australia.
authors.forename: Kenneth
authors.initials: K
authors.lastname: Young
authors.affiliation: Genitourinary Oncology Service, Memorial Sloan Kettering Cancer Center, New York, NY, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Morris
authors.affiliation: Olivia Newton-John Cancer Research Institute, Heidelberg, Australia.
authors.forename: Andrew
authors.initials: A
authors.lastname: Weickhardt
authors.affiliation: Monash University Faculty of Medicine, Nursing and Health Sciences, Monash University, Melbourne, Australia.|Eastern Health, Melbourne, Australia.
authors.forename: Ian D
authors.identifier: 0000-0002-9066-8244
authors.initials: ID
authors.lastname: Davis
authors.affiliation: Department of Molecular Imaging and Therapy, Austin Health, Heidelberg, Australia.|Olivia Newton-John Cancer Research Institute, Heidelberg, Australia.|School of Medicine, Dentistry and Health Sciences, The University of Melbourne, Parkville, Australia.|School of Cancer Medicine, LaTrobe University, Australia.
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Scott
mesh_terms: D001331:Automation; D060326:Chemistry Techniques, Synthetic / Q000379:methods*; D013196:Dihydrotestosterone / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D005462:Fluorine Radioisotopes; D007553:Isotope Labeling; D011786:Quality Control; D011854:Radiochemistry / Q000379:methods*
chemical_list: C494901:16-fluorodihydrotestosterone; D005462:Fluorine Radioisotopes; D013196:Dihydrotestosterone