title: Roles of the novel coiled-coil protein Rng10 in septum formation during fission yeast cytokinesis.
abstract: Rho GAPs are important regulators of Rho GTPases, which are involved in various steps of cytokinesis and other processes. However, regulation of Rho-GAP cellular localization and function is not fully understood. Here we report the characterization of a novel coiled-coil protein Rng10 and its relationship with the Rho-GAP Rga7 in fission yeast. Both rng10Δ and rga7Δ result in defective septum and cell lysis during cytokinesis. Rng10 and Rga7 colocalize on the plasma membrane at the cell tips during interphase and at the division site during cell division. Rng10 physically interacts with Rga7 in affinity purification and coimmunoprecipitation. Of interest, Rga7 localization is nearly abolished without Rng10. Moreover, Rng10 and Rga7 work together to regulate the accumulation and dynamics of glucan synthases for successful septum formation in cytokinesis. Our results show that cellular localization and function of the Rho-GAP Rga7 are regulated by a novel protein, Rng10, during cytokinesis in fission yeast.
authors.affiliation: Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210.
authors.forename: Yajun
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210.
authors.forename: I-Ju
authors.initials: IJ
authors.lastname: Lee
authors.affiliation: Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210.
authors.forename: Mingzhai
authors.initials: M
authors.lastname: Sun
authors.affiliation: Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210.
authors.forename: Casey A
authors.initials: CA
authors.lastname: Lower
authors.affiliation: Department of Molecular Genetics, Cleveland Clinic Lerner College of Medicine, Cleveland, OH 44195.
authors.forename: Kurt W
authors.initials: KW
authors.lastname: Runge
authors.affiliation: Department of Surgery, Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210.
authors.forename: Jianjie
authors.initials: J
authors.lastname: Ma
authors.affiliation: Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210 Department of Biological Chemistry and Pharmacology, The Ohio State University, Columbus, OH 43210 wu.620@osu.edu.
authors.forename: Jian-Qiu
authors.initials: JQ
authors.lastname: Wu
mesh_terms: D002455:Cell Division / Q000502:physiology; D002473:Cell Wall / Q000378:metabolism; D048749:Cytokinesis; D020690:GTPase-Activating Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D005964:Glucosyltransferases / Q000378:metabolism; D020662:Guanine Nucleotide Exchange Factors / Q000235:genetics / Q000378:metabolism; D000072416:Protein Structural Elements; D012568:Schizosaccharomyces / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D020741:rho GTP-Binding Proteins / Q000378:metabolism
chemical_list: D020690:GTPase-Activating Proteins; D020662:Guanine Nucleotide Exchange Factors; C085379:rho GTPase-activating protein; D005964:Glucosyltransferases; C040232:glucan synthase; D020741:rho GTP-Binding Proteins