title: UMI-4C for quantitative and targeted chromosomal contact profiling.
abstract: We developed a targeted chromosome conformation capture (4C) approach that uses unique molecular identifiers (UMIs) to derive high-complexity quantitative chromosome contact profiles with controlled signal-to-noise ratios. UMI-4C detects chromosomal interactions with improved sensitivity and specificity, and it can easily be multiplexed to allow robust comparison of contact distributions between loci and conditions. This approach may open the way to the incorporation of contact distributions into quantitative models of gene regulation.
authors.affiliation: Department of Human Molecular Genetics and Biochemistry, Tel Aviv University, Tel Aviv, Israel.|Functional Genomics and Childhood Leukemia Research Section, Cancer Research Center, Edmond and Lily Safra Children's Hospital, Sheba Medical Center, Ramat-Gan, Israel.|Department of Computer Science and Applied Mathematics, Weizmann Institute, Rehovot, Israel.|Department of Biological Regulation, Weizmann Institute, Rehovot, Israel.
authors.forename: Omer
authors.identifier: 0000-0002-4122-8111
authors.initials: O
authors.lastname: Schwartzman
authors.affiliation: Department of Computer Science and Applied Mathematics, Weizmann Institute, Rehovot, Israel.|Department of Biological Regulation, Weizmann Institute, Rehovot, Israel.
authors.forename: Zohar
authors.initials: Z
authors.lastname: Mukamel
authors.affiliation: Department of Computer Science and Applied Mathematics, Weizmann Institute, Rehovot, Israel.|Department of Biological Regulation, Weizmann Institute, Rehovot, Israel.
authors.forename: Noa
authors.initials: N
authors.lastname: Oded-Elkayam
authors.affiliation: Department of Computer Science and Applied Mathematics, Weizmann Institute, Rehovot, Israel.|Department of Biological Regulation, Weizmann Institute, Rehovot, Israel.
authors.forename: Pedro
authors.initials: P
authors.lastname: Olivares-Chauvet
authors.affiliation: Department of Computer Science and Applied Mathematics, Weizmann Institute, Rehovot, Israel.|Department of Biological Regulation, Weizmann Institute, Rehovot, Israel.
authors.forename: Yaniv
authors.initials: Y
authors.lastname: Lubling
authors.affiliation: Department of Computer Science and Applied Mathematics, Weizmann Institute, Rehovot, Israel.|Department of Biological Regulation, Weizmann Institute, Rehovot, Israel.
authors.forename: Gilad
authors.initials: G
authors.lastname: Landan
authors.affiliation: Department of Human Molecular Genetics and Biochemistry, Tel Aviv University, Tel Aviv, Israel.|Functional Genomics and Childhood Leukemia Research Section, Cancer Research Center, Edmond and Lily Safra Children's Hospital, Sheba Medical Center, Ramat-Gan, Israel.
authors.forename: Shai
authors.initials: S
authors.lastname: Izraeli
authors.affiliation: Department of Computer Science and Applied Mathematics, Weizmann Institute, Rehovot, Israel.|Department of Biological Regulation, Weizmann Institute, Rehovot, Israel.
authors.forename: Amos
authors.initials: A
authors.lastname: Tanay
mesh_terms: D002843:Chromatin / Q000235:genetics; D002874:Chromosome Mapping / Q000379:methods*; D002877:Chromosomes, Human / Q000737:chemistry* / Q000378:metabolism*; D003627:Data Interpretation, Statistical; D005786:Gene Expression Regulation; D056426:Genetic Loci; D023281:Genomics / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D007938:Leukemia / Q000235:genetics*; D009690:Nucleic Acid Conformation; D059629:Signal-To-Noise Ratio; D012984:Software; D014407:Tumor Cells, Cultured; D055544:beta-Globins / Q000235:genetics
chemical_list: D002843:Chromatin; D055544:beta-Globins