title: Biodistribution and toxicity evaluation of sesbania mosaic virus nanoparticles in mice.
abstract: Sesbania mosaic virus (SeMV), a 30-nm spherical plant sobemovirus, is suitable for developing functionalized nanoparticles for biomedical applications. However, the in vivo behavior of SeMV and the clinical impact following its delivery via the oral or intravenous route are not known. To address this question, we examined the biodistribution, toxicity and histopathological changes in SeMV treated mice. No toxic effects were observed in mice administered high doses (100 mg and 200 mg per kg body weight orally or 40 mg and 80 mg per kg body weight intravenously) of SeMV, and they were found to be normal. Analysis of fecal sample showed that SeMV was cleared in 16 h when 20 mg of the virus per kg body weight was administered orally. RT-PCR analysis of blood samples showed that SeMV was present up to 72 h in mice inoculated either intravenously (8 mg/kg body weight) or orally (20 mg/kg body weight). Further, SeMV was found to be localized up to 72 h in spleen and liver tissues of intravenously inoculated mice only. Biochemical and hematological parameters were found to be normal at 6 and 72 h after administration of SeMV. Furthermore, no noticeable changes were observed in histological sections of brain, liver, spleen, lungs and kidney tissue samples collected at 6 and 72 h from SeMV administered mice when compared to control mice. Thus, SeMV appears to be a safe and non-toxic platform that can be tailored as a nanocarrier for in vivo biomedical applications.
authors.affiliation: Department of Virology, Sri Venkateswara University, Tirupati, Andhra Pradesh, India.
authors.forename: G P
authors.initials: GP
authors.lastname: Vishnu Vardhan
authors.affiliation: Department of Biochemistry, Indian Institute of Science, Bengaluru, Karnataka, India.
authors.forename: H S
authors.initials: HS
authors.lastname: Savithri
authors.affiliation: Department of Molecular Biophysics, Indian Institute of Science, Bengaluru, Karnataka, India.
authors.forename: M R N
authors.initials: MR
authors.lastname: Murthy
authors.affiliation: Department of Virology, Sri Venkateswara University, Tirupati, Andhra Pradesh, India. hemamasarapu70@gmail.com.
authors.forename: M
authors.initials: M
authors.lastname: Hema
mesh_terms: D061605:Administration, Intravenous; D000284:Administration, Oral; D000818:Animals; D001769:Blood / Q000821:virology; D005243:Feces / Q000821:virology; D005260:Female; D006651:Histocytochemistry; D007668:Kidney / Q000473:pathology / Q000821:virology; D008099:Liver / Q000473:pathology / Q000821:virology; D051379:Mice; D053758:Nanoparticles / Q000008:administration & dosage / Q000378:metabolism* / Q000633:toxicity*; D010942:Plant Viruses / Q000378:metabolism*; D012328:RNA Viruses / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D049931:Sesbania / Q000821:virology*; D013154:Spleen / Q000473:pathology / Q000821:virology