title: Adsorption and rheological behavior of an amphiphilic protein at oil/water interfaces.
abstract: Hydrophobins are highly surface active proteins which self-assemble at hydrophilic-hydrophobic interfaces into amphipathic membranes. We investigate hydrophobin self-assembly at oil/water interfaces to deepen the understanding of protein behavior in order to improve our biomimetic synthesis. Therefore, we carried out pendant drop measurements of hydrophobin stabilized oil/water systems determining the time-dependent IFT and the dilatational rheology with additional adaptation to the Serrien protein model. We show that the class I hydrophobin H(∗)Protein B adsorbs at an oil/water interface where it forms a densely-packed interfacial protein layer, which dissipates energy during droplet oscillation. Furthermore, the interfacial protein layer exhibits shear thinning behavior.
authors.affiliation: DWI - Leibniz-Institut für Interaktive Materialien e.V., Lehrstuhl für Makromolekulare Materialien und Oberflächen, RWTH Aachen University, Forckenbeckstraße 50, 52056 Aachen, Germany.
authors.forename: Marina J
authors.initials: MJ
authors.lastname: Richter
authors.affiliation: DWI - Leibniz-Institut für Interaktive Materialien e.V., Lehrstuhl für Makromolekulare Materialien und Oberflächen, RWTH Aachen University, Forckenbeckstraße 50, 52056 Aachen, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Schulz
authors.affiliation: Fine Chemical Research, BASF SE, 67056 Ludwigshafen, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Subkowski
authors.affiliation: Fraunhofer-Institut für Angewandte Polymerforschung (IAP), Lehrstuhl für Polymermaterialien und Polymertechnologien, Universität Potsdam, Geiselbergstraße 69, 14476 Potsdam-Golm, Germany. Electronic address: alexander.boeker@iap.fraunhofer.de.
authors.forename: Alexander
authors.initials: A
authors.lastname: Böker
mesh_terms: D000327:Adsorption; D005656:Fungal Proteins / Q000737:chemistry*; D009821:Oils / Q000737:chemistry*; D012212:Rheology; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
chemical_list: D005656:Fungal Proteins; D009821:Oils; D013501:Surface-Active Agents; D014867:Water
keywords: Hydrophobin; IFT; Pendant drop tensiometry; Rheology; Self-assembly