title: Evaluation of a protective effect of in ovo delivered Campylobacter jejuni OMVs.
abstract: Campylobacter jejuni is the most prevalent cause of a food-borne gastroenteritis in the developed world, with poultry being the main source of infection. Campylobacter jejuni, like other Gram-negative bacteria, constitutively releases outer membrane vesicles (OMVs). OMVs are highly immunogenic, can be taken up by mammalian cells, and are easily modifiable by recombinant engineering. We have tested their usefulness for an oral (in ovo) vaccination of chickens. Four groups of 18-day-old chicken embryos (164 animals) underwent injection of wt C. jejuni OMVs or modified OMVs or PBS into the amniotic fluid. The OMVs modifications relied on overexpression of either a complete wt cjaA gene or the C20A mutant that relocates to the periplasm. Fourteen days post-hatch chicks were orally challenged with live C. jejuni strain. Cecum colonization parameters were analyzed by two-way ANOVA with Tukey post-hoc test. The wtOMVs and OMVs with wtCjaA overexpression were found to confer significant protection of chicken against C. jejuni (p = 0.03 and p = 0.013, respectively) in comparison to PBS controls and are promising candidates for further in ovo vaccine development.
authors.affiliation: Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland. renatag@biol.uw.edu.pl.
authors.forename: Renata
authors.identifier: http://orcid.org/0000-0001-7810-8482
authors.initials: R
authors.lastname: Godlewska
authors.affiliation: Department of Epizootiology and Clinic for Birds and Exotic Animals, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Pl. Grunwaldzki 45, 50-366, Wrocław, Poland.
authors.forename: Maciej
authors.initials: M
authors.lastname: Kuczkowski
authors.affiliation: Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Wyszyńska
authors.affiliation: Department of Epizootiology and Clinic for Birds and Exotic Animals, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Pl. Grunwaldzki 45, 50-366, Wrocław, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Klim
authors.affiliation: Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Derlatka
authors.affiliation: Department of Epizootiology and Clinic for Birds and Exotic Animals, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Pl. Grunwaldzki 45, 50-366, Wrocław, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Woźniak-Biel
authors.affiliation: Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland.
authors.forename: Elżbieta K
authors.initials: EK
authors.lastname: Jagusztyn-Krynicka
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D058491:Bacterial Load; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000276:immunology*; D002169:Campylobacter Infections / Q000517:prevention & control / Q000662:veterinary*; D016123:Campylobacter jejuni / Q000276:immunology*; D002432:Cecum / Q000382:microbiology; D002645:Chickens; D000067128:Extracellular Vesicles / Q000378:metabolism*; D016896:Treatment Outcome
chemical_list: D000942:Antigens, Bacterial; D001428:Bacterial Vaccines
keywords: Campylobacter jejuni; Outer membrane vesicles (OMV); in ovo immunization