title: Genetic diversity of Mycobacterium tuberculosis isolates from central India.
abstract: OBJECTIVE
  There is a paucity of data available on genetic biodiversity of Mycobacterium tuberculosis isolates from central India. The present study was carried out on isolates of M. tuberculosis cultured from diagnostic clinical samples of patients from Bhopal, central India, using spoligotyping as a method of molecular typing.
  
  
  METHODS
  DNA was extracted from 340 isolates of M. tuberculosis from culture, confirmed as M. tuberculosis by molecular and biochemical methods and subjected to spoligotyping. The results were compared with the international SITVIT2 database.
  
  
  RESULTS
  Sixty five different spoligo international type (SIT) patterns were observed. A total of 239 (70.3%) isolates could be clustered into 25 SITs. The Central Asian (CAS) and East African Indian (EAI) families were found to be the two major circulating families in this region. SIT26/CAS1_DEL was identified as the most predominant type, followed by SIT11/EAI3_IND and SIT288/CAS[2]. Forty (11.8%) unique (non-clustered) and 61 (17.9%) orphan isolates were identified in the study. There was no significant association of clustering with clinical and demographic characteristics of patients.
  
  
  CONCLUSIONS
  Well established SITs were found to be predominant in our study. SIT26/CAS1_DEL was the most predominant type. However, the occurrence of a substantial number of orphan isolates may indicate the presence of active spatial and temporal evolutionary dynamics within the isolates of M. tuberculosis.
authors.affiliation: Bhopal Memorial Hospital & Research Centre (ICMR), Bhopal, Madhya Pradesh, India.
authors.forename: Prabha
authors.initials: P
authors.lastname: Desikan
authors.affiliation: National JALMA Institute of Leprosy & Other Mycobacterial Diseases (ICMR), Agra, India.
authors.forename: D S
authors.initials: DS
authors.lastname: Chauhan
authors.affiliation: National JALMA Institute of Leprosy & Other Mycobacterial Diseases (ICMR), Agra, India.
authors.forename: Pragya
authors.initials: P
authors.lastname: Sharma
authors.affiliation: Bhopal Memorial Hospital & Research Centre (ICMR), Bhopal, Madhya Pradesh, India.
authors.forename: Nikita
authors.initials: N
authors.lastname: Panwalkar
authors.affiliation: Bhopal Memorial Hospital & Research Centre (ICMR), Bhopal, Madhya Pradesh, India.
authors.forename: Manju
authors.initials: M
authors.lastname: Chourey
authors.affiliation: Bhopal Memorial Hospital & Research Centre (ICMR), Bhopal, Madhya Pradesh, India.
authors.forename: Mohan Lal
authors.initials: ML
authors.lastname: Patidar
authors.affiliation: National JALMA Institute of Leprosy & Other Mycobacterial Diseases (ICMR), Agra, India.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Yadav
authors.affiliation: National Institute for Research in Tuberculosis (ICMR), Chennai, India.
authors.forename: V
authors.initials: V
authors.lastname: Chandrasekaran
authors.affiliation: State TB Office, Directorate of Health Services, Bhopal, Madhya Pradesh, India.
authors.forename: B S
authors.initials: BS
authors.lastname: Ohri
mesh_terms: D000293:Adolescent; D015373:Bacterial Typing Techniques; D002648:Child; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007194:India; D008297:Male; D008875:Middle Aged; D018598:Minisatellite Repeats / Q000235:genetics; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D014376:Tuberculosis / Q000235:genetics / Q000382:microbiology*; D055815:Young Adult