title: A suite of pulse sequences based on multiple sequential acquisitions at one and two radiofrequency channels for solid-state magic-angle spinning NMR studies of proteins.
abstract: One of the fundamental challenges in the application of solid-state NMR is its limited sensitivity, yet a majority of experiments do not make efficient use of the limited polarization available. The loss in polarization in a single acquisition experiment is mandated by the need to select out a single coherence pathway. In contrast, sequential acquisition strategies can encode more than one pathway in the same experiment or recover unused polarization to supplement a standard experiment. In this article, we present pulse sequences that implement sequential acquisition strategies on one and two radiofrequency channels with a combination of proton and carbon detection to record multiple experiments under magic-angle spinning. We show that complementary 2D experiments such as [Formula: see text] and [Formula: see text] or DARR and [Formula: see text], and 3D experiments such as [Formula: see text] and [Formula: see text], or [Formula: see text] and [Formula: see text]  can be combined in a single experiment to ensure time savings of at least 40 %. These experiments can be done under fast or slow-moderate magic-angle spinning frequencies aided by windowed [Formula: see text] acquisition and homonulcear decoupling. The pulse sequence suite is further expanded by including pathways that allow the recovery of residual polarization, the so-called 'afterglow' pathways, to encode a number of pulse sequences to aid in assignments and chemical-shift mapping.
authors.affiliation: TIFR Centre for Interdisciplinary Sciences, Tata Institute of Fundamental Research, Hyderabad, 21, Brundavan Colony, Narsingi, Hyderabad, 500 075, India.
authors.forename: Kshama
authors.initials: K
authors.lastname: Sharma
authors.affiliation: TIFR Centre for Interdisciplinary Sciences, Tata Institute of Fundamental Research, Hyderabad, 21, Brundavan Colony, Narsingi, Hyderabad, 500 075, India. madhu@tifr.res.in.|Department of Chemical Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai, 400 005, India. madhu@tifr.res.in.
authors.forename: Perunthiruthy K
authors.initials: PK
authors.lastname: Madhu
authors.affiliation: TIFR Centre for Interdisciplinary Sciences, Tata Institute of Fundamental Research, Hyderabad, 21, Brundavan Colony, Narsingi, Hyderabad, 500 075, India. kaustuberm@tifrh.res.in.
authors.forename: Kaustubh R
authors.identifier: http://orcid.org/0000-0002-5151-3987
authors.initials: KR
authors.lastname: Mote
mesh_terms: D002244:Carbon / Q000737:chemistry; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D007553:Isotope Labeling; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D011506:Proteins / Q000737:chemistry*; D066244:Proton Magnetic Resonance Spectroscopy; D011522:Protons
chemical_list: D011506:Proteins; D011522:Protons; D002244:Carbon
keywords: Magic-angle spinning solid-state NMR; Multiple receivers; Multiple sequential acquisition; Proton detection