title: NiCoMnO4: A Bifunctional Affinity Probe for His-Tagged Protein Purification and Phosphorylation Sites Recognition.
abstract: A bifunctional affinity probe NiCoMnO4 was designed and prepared with controllable morphology and size using facile methods. It was observed that the probe could be applied in His-tagged proteins purification and phosphopeptides enrichment simply through the buffer modulation. NiCoMnO4 particles showed satisfactory cycling performance for His-tagged proteins purification and broad pH-tolerance of loading buffer for phosphopeptides affinity. Therefore, a high-throughput, cost-effective, and efficient protein/peptide purification method was developed within 10 min based on the novel bifunctional affinity probe.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University , Beijing 100871, China.
authors.forename: Xiaoyue
authors.initials: X
authors.lastname: Qi
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University , Beijing 100871, China.
authors.forename: Long
authors.initials: L
authors.lastname: Chen
authors.affiliation: Beijing Nuclear Magnetic Resonance Center, College of Life Science, Peking University , Beijing 100871, China.
authors.forename: Chaoqun
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University , Beijing 100871, China.
authors.forename: Xinyuan
authors.initials: X
authors.lastname: Xu
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University , Beijing 100871, China.
authors.forename: Yiding
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University , Beijing 100871, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University , Beijing 100871, China.
authors.forename: Huwei
authors.initials: H
authors.lastname: Liu
mesh_terms: D002846:Chromatography, Affinity; D006639:Histidine; D007202:Indicators and Reagents; D017895:Manganese Compounds / Q000737:chemistry*; D010748:Phosphopeptides; D010766:Phosphorylation; D011506:Proteins
chemical_list: D007202:Indicators and Reagents; D017895:Manganese Compounds; D010748:Phosphopeptides; D011506:Proteins; D006639:Histidine
keywords: His-tagged proteins purification; affinity probes; buffer modulation; metal oxide; phosphopeptides enrichment