title: [Use of dried blood spots in early diagnosis of HIV-1 infection in children born to HIV-infected mothers as part of the prevention of mother-to-child transmission in Benin].
abstract: The goal of this study was to evaluate using the molecular diagnosis, infection transmission rate of HIV in children born to HIV-1 positive mothers as part of the prevention of mother-to-child transmission (PMTCT) in Benin. The sample consisted of 524 dried blood spots (DBS) of children born to HIV-1 positive mothers, from 30 sites (PMTCT) taken between October 2009 and June 2010. The diagnosis of HIV-1 was performed by the qualitative detection of viral nucleic acids (RNA and DNA) in DBS on filter paper using the Abbott RealTime(®) HIV-1 Qualitative assay. We found that 51 DBS were positive (9.7%) and 473 were negative (90.3%). The failure rate of PMTCT among 420 mothers who received antiretroviral prophylaxis was 6.7% (28/420). This failure rate was significantly higher among children born to infected mothers on antiretroviral monotherapy than on triple therapy (HAART). The results of our study enrich the data in the literature on highly active antiretroviral chemoprophylaxis to reduce the transmission of HIV-1 from mother to child.
authors.affiliation: Laboratoire de référence du Programme national de lutte contre le sida, direction nationale de la Santé publique, ministère de la Santé, 04 BP 378, Cotonou, Bénin. tchiakpeedmond211170@gmail.com.|Laboratoire de bactériologie et de virologie, université Cheikh-Anta-Diop, BP 7325, 30, av Pasteur, Dakar, Sénégal. tchiakpeedmond211170@gmail.com.|Laboratoire de biochimie et de biologie moléculaire de l'université d'Abomey-Calavi, 04 BP 0320, Cotonou, Bénin. tchiakpeedmond211170@gmail.com.
authors.forename: E
authors.initials: E
authors.lastname: Tchiakpe
authors.affiliation: Laboratoire de référence du Programme national de lutte contre le sida, direction nationale de la Santé publique, ministère de la Santé, 04 BP 378, Cotonou, Bénin.
authors.forename: A
authors.initials: A
authors.lastname: Hounto-Ogouyemi
authors.affiliation: Laboratoire de bactériologie et de virologie, université Cheikh-Anta-Diop, BP 7325, 30, av Pasteur, Dakar, Sénégal.
authors.forename: H
authors.initials: H
authors.lastname: Diop Ndiaye
authors.affiliation: Laboratoire de bactériologie et de virologie, université Cheikh-Anta-Diop, BP 7325, 30, av Pasteur, Dakar, Sénégal.
authors.forename: A A M
authors.initials: AA
authors.lastname: Diouara
authors.affiliation: Laboratoire de référence du Programme national de lutte contre le sida, direction nationale de la Santé publique, ministère de la Santé, 04 BP 378, Cotonou, Bénin.
authors.forename: A K
authors.initials: AK
authors.lastname: Aïssi
authors.affiliation: Laboratoire de référence du Programme national de lutte contre le sida, direction nationale de la Santé publique, ministère de la Santé, 04 BP 378, Cotonou, Bénin.
authors.forename: R K
authors.initials: RK
authors.lastname: Keke
authors.affiliation: Service de médecine interne du centre hospitalier universitaire de Parakou, BP 02, Parakou, Bénin.
authors.forename: A A
authors.initials: AA
authors.lastname: Kpangon
authors.affiliation: Laboratoire de référence du Programme national de lutte contre le sida, direction nationale de la Santé publique, ministère de la Santé, 04 BP 378, Cotonou, Bénin.
authors.forename: B
authors.initials: B
authors.lastname: Lafia
authors.affiliation: Laboratoire de référence du Programme national de lutte contre le sida, direction nationale de la Santé publique, ministère de la Santé, 04 BP 378, Cotonou, Bénin.
authors.forename: D
authors.initials: D
authors.lastname: Métadokou
authors.affiliation: Laboratoire de référence du Programme national de lutte contre le sida, direction nationale de la Santé publique, ministère de la Santé, 04 BP 378, Cotonou, Bénin.
authors.forename: B
authors.initials: B
authors.lastname: Bouraïma
authors.affiliation: Laboratoire de référence du Programme national de lutte contre le sida, direction nationale de la Santé publique, ministère de la Santé, 04 BP 378, Cotonou, Bénin.
authors.forename: D
authors.initials: D
authors.lastname: Anthony
authors.affiliation: Laboratoire de référence du Programme national de lutte contre le sida, direction nationale de la Santé publique, ministère de la Santé, 04 BP 378, Cotonou, Bénin.
authors.forename: A
authors.initials: A
authors.lastname: Hounsinou
authors.affiliation: Service de pédiatrie et génétique médicale du centre national hospitalier et universitaire de Cotonou, 01 BP 386, Cotonou, Bénin.
authors.forename: M J
authors.initials: MJ
authors.lastname: Alao
authors.affiliation: Laboratoire de référence du Programme national de lutte contre le sida, direction nationale de la Santé publique, ministère de la Santé, 04 BP 378, Cotonou, Bénin.
authors.forename: A
authors.initials: A
authors.lastname: Azondekon
authors.affiliation: Service de pédiatrie, hôpital d'instruction des armées (HIA), 01 BP 517, Cotonou, Bénin.
authors.forename: A D
authors.initials: AD
authors.lastname: Ahouidi
authors.affiliation: Laboratoire de référence du Programme national de lutte contre le sida, direction nationale de la Santé publique, ministère de la Santé, 04 BP 378, Cotonou, Bénin.
authors.forename: A K
authors.initials: AK
authors.lastname: Bei
authors.affiliation: Laboratoire de bactériologie et de virologie, université Cheikh-Anta-Diop, BP 7325, 30, av Pasteur, Dakar, Sénégal.
authors.forename: M A S
authors.initials: MA
authors.lastname: Mbengue
authors.affiliation: Laboratoire de bactériologie et de virologie, université Cheikh-Anta-Diop, BP 7325, 30, av Pasteur, Dakar, Sénégal.
authors.forename: C
authors.initials: C
authors.lastname: Touré Kane
authors.affiliation: Centre de traitement ambulatoire (CTA) du centre national hospitalier et universitaire de Cotonou, 01 BP 386, Cotonou, Bénin.
authors.forename: D M
authors.initials: DM
authors.lastname: Zannou
mesh_terms: D044966:Anti-Retroviral Agents / Q000627:therapeutic use; D001541:Benin / Q000453:epidemiology; D018890:Chemoprevention; D059788:Dried Blood Spot Testing / Q000379:methods*; D042241:Early Diagnosis; D005260:Female; D015658:HIV Infections / Q000151:congenital / Q000175:diagnosis* / Q000188:drug therapy / Q000453:epidemiology; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007232:Infant, Newborn, Diseases / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology; D018445:Infectious Disease Transmission, Vertical / Q000517:prevention & control* / Q000706:statistics & numerical data; D008297:Male; D025202:Molecular Diagnostic Techniques / Q000379:methods; D009035:Mothers; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000097:blood / Q000175:diagnosis / Q000453:epidemiology
chemical_list: D044966:Anti-Retroviral Agents
keywords: Antiretroviral prophylaxis; Benin; Breastfeeding; Early diagnosis; HIV-1; Mother-to-child transmission; Sub-Saharan Africa