title: Immobilization of Candida antarctica lipase B onto SBA-15 and their application in glycerolysis for diacylglycerols synthesis.
abstract: In this study, Candida antarctica lipase B (CALB) was immobilized on SBA-15 with three pore diameters. CALB loading was found increased with CALB concentration increasing from 20.3 to 80.12μg/ml. Higher CALB loading was observed from SBA-15 with pore diameters at 8.1nm (SBA-15(8.1)), yet highest hydrolytic activity was found at SBA-15(12.5). Thermal stability of the immobilized CALB (SBA-15-CALB) samples was greatly influenced by their water content, after 4h storage at 70°C, 8.93 and 67.4% of the initial activity was observed from SBA-15-CALB samples with water content at 9.23 and 3.22% respectively. The SBA-15-CALB samples were then used in glycerolysis of corn oil, and 53.6wt% of diacylglycerols was obtained after optimization. The operational stability was tested, and after 5 consecutive applications, 92.5 and 80.3% of the initial glycerolysis activity was remained respectively from SBA-15(6.6)-CALB and SBA-15(12.5)-CALB.
authors.affiliation: School of Food Science, Guangdong Pharmaceutical University, Zhongshan 528458, China.
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Cai
authors.affiliation: School of Food Science, Guangdong Pharmaceutical University, Zhongshan 528458, China.
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Henan Province Product Quality Supervision and Inspection Center, Zhengzhou 450004, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Food Science, Guangdong Pharmaceutical University, Zhongshan 528458, China. Electronic address: adong473@163.com.
authors.forename: Nanjing
authors.initials: N
authors.lastname: Zhong
authors.affiliation: School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
mesh_terms: D004075:Diglycerides / Q000138:chemical synthesis*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005656:Fungal Proteins / Q000302:isolation & purification*; D008049:Lipase / Q000302:isolation & purification*; D012822:Silicon Dioxide / Q000378:metabolism*
chemical_list: D004075:Diglycerides; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; C509969:SBA-15; D012822:Silicon Dioxide; D008049:Lipase; C431349:lipase B, Candida antarctica
keywords: Candida antarctica lipase B; Diacylglycerols; Glycerolysis; Immobilization; SBA-15