title: Bio-remediation of acephate-Pb(II) compound contaminants by Bacillus subtilis FZUL-33.
abstract: Removal of Pb(2+) and biodegradation of organophosphorus have been both widely investigated respectively. However, bio-remediation of both Pb(2+) and organophosphorus still remains largely unexplored. Bacillus subtilis FZUL-33, which was isolated from the sediment of a lake, possesses the capability for both biomineralization of Pb(2+) and biodegradation of acephate. In the present study, both Pb(2+) and acephate were simultaneously removed via biodegradation and biomineralization in aqueous solutions. Batch experiments were conducted to study the influence of pH, interaction time and Pb(2+) concentration on the process of removal of Pb(2+). At the temperature of 25°C, the maximum removal of Pb(2+) by B.subtilis FZUL-33 was 381.31±11.46mg/g under the conditions of pH5.5, initial Pb(2+) concentration of 1300mg/L, and contact time of 10min. Batch experiments were conducted to study the influence of acephate on removal of Pb(2+) and the influence of Pb(2+) on biodegradation of acephate by B.subtilis FZUL-33. In the mixed system of acephate-Pb(2+), the results show that biodegradation of acephate by B.subtilis FZUL-33 released PO4(3+), which promotes mineralization of Pb(2+). The process of biodegradation of acephate was affected slightly when the concentration of Pb(2+) was below 100mg/L. Based on the results, it can be inferred that the B.subtilis FZUL-33 plays a significant role in bio-remediation of organophosphorus-heavy metal compound contamination.
authors.affiliation: The College of Environment and Resources, Fuzhou University, Fuzhou 350108, China. Electronic address: jasmine1113@hotmail.com.
authors.forename: Wenting
authors.initials: W
authors.lastname: Lin
authors.affiliation: The College of Environment and Resources, Fuzhou University, Fuzhou 350108, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Huang
authors.affiliation: The College of Environment and Resources, Fuzhou University, Fuzhou 350108, China.
authors.forename: Xuezhen
authors.initials: X
authors.lastname: Li
authors.affiliation: The College of Environment and Resources, Fuzhou University, Fuzhou 350108, China.
authors.forename: Minghua
authors.initials: M
authors.lastname: Liu
authors.affiliation: The College of Environment and Resources, Fuzhou University, Fuzhou 350108, China. Electronic address: yjcheng@fzu.edu.cn.
authors.forename: Yangjian
authors.initials: Y
authors.lastname: Cheng
mesh_terms: D001412:Bacillus subtilis / Q000378:metabolism*; D001673:Biodegradation, Environmental; D007854:Lead / Q000378:metabolism*; D009946:Organothiophosphorus Compounds / Q000378:metabolism*; D063088:Phosphoramides / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D009946:Organothiophosphorus Compounds; D063088:Phosphoramides; D014874:Water Pollutants, Chemical; D007854:Lead; C001969:acephate
keywords: Acephate–Pb(2+); Bacillus subtilis; Biodegradation; Biomineralization