title: Histological study on the protective role of vitamin B complex on the cerebellum of diabetic rat.
abstract: BACKGROUND
  Disorder in cerebellar structure was reported in diabetes mellitus. B vitamins are involved in many significant metabolic processes within the brain.
  
  
  OBJECTIVE
  To detect the protective role of vitamin B complex on the histological structure of the cerebellum of experimentally induced diabetic rat.
  
  
  METHODS
  Eighteen adult male Wistar rats were divided into two groups. Group I: normal vehicle control (n=6). Group II: streptozotocin-induced diabetic rats (n=12), which was equally divided into two subgroups; IIA (diabetic vehicle control), IIB (diabetic vitamin B complex-treated): streptozotocin-induced diabetic rats treated with vitamin B complex (1mg/kg/day) for 6 weeks. Specimens from the cerebellum were processed for light and electron microscopy.
  
  
  RESULTS
  In vitamin B complex treated group, the histological changes in Purkinje cells, astrocytes and oligodendrocytes were improved compared with the diabetic non-treated group. The white matter revealed intact myelinated axons. Inducible nitric oxide synthase (iNOS) and caspase-3 expression reduced. Glial fibrillary acidic protein (GFAP) expression revealed less activated astroglia. The number of Purkinje cells/mm(2) significantly increased. While, the number of GFAP positive astrocytes/mm(2) significantly decreased. In addition, the blood glucose level was reduced.
  
  
  CONCLUSIONS
  Vitamin B complex protected the cerebellum from the histological changes which occurred in STZ- induced diabetic rats.
authors.affiliation: Histology Department, Faculty of Medicine, Assiut University, Egypt. Electronic address: sohair_eltony@yahoo.com.
authors.forename: Sohair A
authors.initials: SA
authors.lastname: Eltony
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000648:ultrastructure; D001369:Axons / Q000187:drug effects / Q000648:ultrastructure; D001786:Blood Glucose; D053148:Caspase 3 / Q000096:biosynthesis; D002531:Cerebellum / Q000187:drug effects* / Q000648:ultrastructure; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000473:pathology; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis; D011689:Purkinje Cells / Q000187:drug effects / Q000648:ultrastructure; D051381:Rats; D014803:Vitamin B Complex / Q000008:administration & dosage*; D066127:White Matter / Q000187:drug effects / Q000648:ultrastructure
chemical_list: D001786:Blood Glucose; D014803:Vitamin B Complex; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; D053148:Caspase 3
keywords: Diabetic encephalopathy; Gliosis; Vitamin B complex