title: Recyclable Strategy for the Production of High-Purity Galacto-oligosaccharides by Kluyveromyces lactis.
abstract: A recyclable strategy for the production of high-purity (>95%) galacto-oligosaccharides (GOS) was developed using Kluyveromyces lactis in both the synthesis and purification steps. For the synthesis of GOS, ethanol-permeabilized cells (p-cells) of K. lactis were used because the enhanced permeability facilitated the mass transfer of the substrate and the release of oligosaccharide products. For the purification of GOS, non-permeabilized K. lactis cells (np-cells) were preferred as a result of their intrinsic cell membrane barrier toward GOS, which led to the selective consumption of carbohydrate. In this way, undesired glucose, galactose, and lactose in the raw GOS solution can be completely removed. This strategy is recyclable not only because of the high stability and reusability of p-cells and np-cells but also because the ethanol, which is simultaneously generated during the purification, can be reused for the preparation of p-cells. The strategy proposed in this study is a promising candidate for the efficient production of high-purity GOS.
authors.affiliation: State Key Laboratory of Chemical Engineering, Tianjin Key Laboratory of Membrane Science and Desalination Technology, School of Chemical Engineering and Technology, Tianjin University , Tianjin 300072, People's Republic of China.
authors.forename: Huaisheng
authors.initials: H
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Chemical Engineering, Tianjin Key Laboratory of Membrane Science and Desalination Technology, School of Chemical Engineering and Technology, Tianjin University , Tianjin 300072, People's Republic of China.
authors.forename: Shengping
authors.initials: S
authors.lastname: You
authors.affiliation: State Key Laboratory of Chemical Engineering, Tianjin Key Laboratory of Membrane Science and Desalination Technology, School of Chemical Engineering and Technology, Tianjin University , Tianjin 300072, People's Republic of China.
authors.forename: Mengfan
authors.initials: M
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Chemical Engineering, Tianjin Key Laboratory of Membrane Science and Desalination Technology, School of Chemical Engineering and Technology, Tianjin University , Tianjin 300072, People's Republic of China.|Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) , Tianjin 300072, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qi
authors.affiliation: State Key Laboratory of Chemical Engineering, Tianjin Key Laboratory of Membrane Science and Desalination Technology, School of Chemical Engineering and Technology, Tianjin University , Tianjin 300072, People's Republic of China.|Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) , Tianjin 300072, People's Republic of China.
authors.forename: Rongxin
authors.initials: R
authors.lastname: Su
authors.affiliation: State Key Laboratory of Chemical Engineering, Tianjin Key Laboratory of Membrane Science and Desalination Technology, School of Chemical Engineering and Technology, Tianjin University , Tianjin 300072, People's Republic of China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: He
mesh_terms: D000431:Ethanol / Q000378:metabolism; D005690:Galactose / Q000378:metabolism*; D007218:Industrial Microbiology; D007716:Kluyveromyces / Q000378:metabolism*; D007785:Lactose / Q000378:metabolism; D008970:Molecular Weight; D009844:Oligosaccharides / Q000096:biosynthesis*
chemical_list: D009844:Oligosaccharides; D000431:Ethanol; D007785:Lactose; D005690:Galactose
keywords: Kluyveromyces lactis; galacto-oligosaccharides; permeabilized cells; purification; recyclable process