title: Identification of Eimeria acervulina conoid antigen using chicken monoclonal antibody.
abstract: In the poultry industry, Eimeria spp. is one of the important pathogens which cause significant economic losses. We have previously generated a chicken monoclonal antibody (mAb), 6D-12-G10, with specificity for an antigen located in the apical cytoskeleton of Eimeria acervulina and with cross-reactive among Apicomplexan parasites, including other Eimeria spp., Toxoplasma, Neospora, and Cryptosporidium spp. Furthermore, the protein of Cryptosporidium parvum recognized by the 6D-12-G10 has been identified as elongation factor-1α (EF-1α). In the present study, to identify the target molecule of E. acervulina by the mAb, we performed two-dimensional Western blotting analysis. Finally, we found two positive molecules which are identified as EF-1α and a related protein. Our previous finding using C. parvum and the results in this study suggest that EF-1α could be associated with the invasion facilitated by the cytoskeleton at the apical region of zoites.
authors.affiliation: Division of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku Orai Kita, Izumisano, Osaka, 598-8531, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Matsubayashi
authors.affiliation: Division of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku Orai Kita, Izumisano, Osaka, 598-8531, Japan.
authors.forename: Chisa
authors.initials: C
authors.lastname: Minoura
authors.affiliation: Division of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku Orai Kita, Izumisano, Osaka, 598-8531, Japan.
authors.forename: Shintaro
authors.initials: S
authors.lastname: Kimura
authors.affiliation: Division of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku Orai Kita, Izumisano, Osaka, 598-8531, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tani
authors.affiliation: Division of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku Orai Kita, Izumisano, Osaka, 598-8531, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Furuya
authors.affiliation: Animal Biosciences and Biotechnology Laboratory, Agricultural Research Services, United States Department of Agriculture, Beltsville, MD, 20705, USA.
authors.forename: Hyun S
authors.initials: HS
authors.lastname: Lillehoj
authors.affiliation: Laboratory of Immunobiology, Department of Molecular and Applied Biosciences, Graduate School of Biosphere Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi, Hiroshima, 739-8528, Japan.
authors.forename: Haruo
authors.initials: H
authors.lastname: Matsuda
authors.affiliation: Division of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku Orai Kita, Izumisano, Osaka, 598-8531, Japan.
authors.forename: Shigeo
authors.initials: S
authors.lastname: Takenaka
authors.affiliation: Department of Parasitology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa, 252-0374, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Hatta
authors.affiliation: Department of Parasitology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa, 252-0374, Japan.
authors.forename: Naotoshi
authors.initials: N
authors.lastname: Tsuji
authors.affiliation: Division of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku Orai Kita, Izumisano, Osaka, 598-8531, Japan. ksasai@vet.osakafu-u.ac.jp.
authors.forename: Kazumi
authors.initials: K
authors.lastname: Sasai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000913:Antibodies, Protozoan / Q000276:immunology; D000953:Antigens, Protozoan / Q000276:immunology*; D015153:Blotting, Western / Q000662:veterinary; D002645:Chickens / Q000469:parasitology*; D003048:Coccidiosis / Q000469:parasitology / Q000662:veterinary*; D003429:Cross Reactions; D016785:Cryptosporidium parvum / Q000276:immunology / Q000302:isolation & purification; D004539:Eimeria / Q000276:immunology* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D018688:Neospora / Q000276:immunology / Q000302:isolation & purification; D020648:Peptide Elongation Factor 1 / Q000378:metabolism*; D011201:Poultry Diseases / Q000469:parasitology*; D034101:Sporozoites; D014122:Toxoplasma / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D020648:Peptide Elongation Factor 1
keywords: Chicken monoclonal antibody; Conoid; Eimeria acervulina; Elongation factor-1α