title: A novel paradigm for the high-efficient production of phycocyanin from Galdieria sulphuraria.
abstract: A novel cultivation strategy called "Sequential Heterotrophy-Dilution-Photoinduction" was successfully applied in the cultivation of Galdieria sulphuraria for efficient production of phycocyanin (PC). Algae cells were firstly cultivated heterotrophically to achieve high cell density. The maximal dry cell weight of 17.8gL(-1) and maximal biomass productivity of 103.1mgL(-1)h(-1) were obtained. Then, a dilution step was applied to obtain a suitable cell concentration and finally these cells were transferred to high light condition for phycocyanin accumulation. During the photoinduction step, cells could accumulate phycocyanin up to 13.88% of dry cell weight in a cultivation period of 8days. By this technology, total PC productivity far surpassed those reported in all literatures of Galdieria sulphuraria, and was 147-fold and 12-fold of those in photoautotrophic and heterotrophic technologies, respectively. Therefore, this strategy provides a promising approach for high-efficient phycocyanin production from Galdieria sulphuraria to meet its mass cultivation and commercialization application.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Minxi
authors.initials: M
authors.lastname: Wan
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Zhenyang
authors.initials: Z
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Yunnan Shangrila Zeyuan Limited Company for Algal Health Technology, Diqing 674412, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Jiaxing Zeyuan Bio-Products Company Ltd., Jiaxing 314006, PR China.
authors.forename: Shulan
authors.initials: S
authors.lastname: Li
authors.affiliation: Yunnan Shangrila Zeyuan Limited Company for Algal Health Technology, Diqing 674412, PR China.
authors.forename: Anquan
authors.initials: A
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address: ygli@ecust.edu.cn.
authors.forename: Yuanguang
authors.initials: Y
authors.lastname: Li
mesh_terms: D018533:Biomass; D052836:Heterotrophic Processes; D010798:Phycocyanin / Q000096:biosynthesis*; D000461:Rhodophyta / Q000254:growth & development / Q000378:metabolism*
chemical_list: D010798:Phycocyanin
keywords: Galdieria sulphuraria; Phycocyanin; Sequential Heterotrophy-Dilution-Photoinduction