title: MicroRNA-190 regulates FOXP2 genes in human gastric cancer.
abstract: OBJECTIVE
  To investigate how microRNA-190 (miR-190) regulates FOXP2 genes in gastric cancer (GC) cell line SGC7901.
  
  
  METHODS
  We identified that miR-190 could target FOXP2 genes by using dual luciferase enzyme assay. Precursor fragment transfection of miR-190 was performed with GC cell line SGC7901 and human gastric mucosal cell line GES-1. miR-190 expression was detected by reverse transcription-polymerase chain reaction (RT-PCR) and FOXP2 protein expression was measured by Western blotting.
  
  
  RESULTS
  FOXP2-3'-untranslated region (UTR) in miR-190 transfection group was significantly decreased as compared with other groups. There were no significant differences in fluorescence signals of FOXP2mut-3'-UTR in each group. Therefore, it was assumed that miR-190 can target FOXP2 genes. Through RT-PCR verification, it was observed that the expression level of miR-190 was significantly higher in GC cell line SGC7901 than in human gastric mucosa cell line GES-1 after transfection with miR-190 mimics. The expression level of miR-190 was significantly higher in GES-1 cells than in SGC7901 cells after transfection with miR-190 inhibitors. Western blotting results showed the expression level of FOXP2 was significantly lower in GC cell line SGC7901 than in GES-1 cells. Compared with blank, mimics control, and inhibitors control groups, the miR-190 mimics group showed significantly enhanced proliferation, migration, and invasion abilities, while miR-190 inhibitors group showed decreased abilities toward proliferation, migration, and invasion (P<0.05). The transcription level of miR-190 and the expression level of FOXP2 in tumor tissues and adjacent normal tissues in GC patients were verified to be consistent with those of cell line experiments.
  
  
  CONCLUSIONS
  Upregulation of miR-190 can lead to downregulation of FOXP2 protein expression. miR-190 may serve as a potential target for GC diagnosis.
authors.affiliation: Department of General Surgery.
authors.forename: Wen-Zhuo
authors.initials: WZ
authors.lastname: Jia
authors.affiliation: Department of General Surgery.
authors.forename: Tao
authors.initials: T
authors.lastname: Yu
authors.affiliation: Department of General Surgery.
authors.forename: Qi
authors.initials: Q
authors.lastname: An
authors.affiliation: Department of General Surgery.
authors.forename: Hua
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Gastroenterology, Beijing Hospital, Beijing, People's Republic of China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Gastroenterology, Beijing Hospital, Beijing, People's Republic of China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of General Surgery.
authors.forename: Gang
authors.initials: G
authors.lastname: Xiao
keywords: CCK-8; RT-PCR; SGC7901 cell line; Western blotting; migration assay; targeted regulation; transwell