title: Radio sensitizing effect of aloe polysaccharide on pancreatic cancer bxpc-3 cells.
abstract: The roles of autophagy in pancreatic cancers are complex and may differ depending on tumor type or context. This study aimed to investigate if aloe polysaccharide can be used as a radio-sensitizing chemical to induce autophagy in pancreatic cancer cells. Pancreatic carcinoma BxPC-3 cells were divided into four groups: A control group, an aloe polysaccharide only group, a radiation only group and a radiation combined with aloe polysaccharide group (combination group). Transmission electron microscopy was used to observe ultra structural changes of autophagosomes in each cell group. The mRNA expressions of ULK1, GABARAPL1, BECN1, and BCL-2 were detected by real-time PCR. Autophagosomes or autophagolysosomes were observed in all experimental groups except the control group. Compared with the control group, ULK1 mRNA expression was up-regulated and BECN1 and BCL-2 mRNA expression were down-regulated in all groups (P<0.05); changes in expression were most obvious in the combination group (P<0.05). GABARAPL1 mRNA expression was up-regulated in the combination group (P<0.05), but had no significant changes among other groups. In brief, aloe polysaccharide induces autophagy in pancreatic carcinoma BxPC-3 cells both alone and in concert with radiation. Autophagic cell death may be one of the mechanisms producing a radiosensitizing effect.
authors.affiliation: Department of Gastroenterology, the First People's Hospital of Shangqiu City, Shangqiu, Henan Province, P. R. China / Department of Gastroenterology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan Province, P. R. China.
authors.forename: Chunjin
authors.initials: C
authors.lastname: Xu
authors.affiliation: Department of Gastroenterology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan Province, P. R. China.
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics; D000504:Aloe / Q000737:chemistry*; D001343:Autophagy / Q000187:drug effects; D045744:Cell Line, Tumor; D006801:Humans; D008854:Microscopy, Electron; D008869:Microtubule-Associated Proteins / Q000235:genetics; D010190:Pancreatic Neoplasms / Q000473:pathology / Q000532:radiotherapy* / Q000648:ultrastructure; D011134:Polysaccharides / Q000494:pharmacology*; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C430503:GABARAPL1 protein, human; D008869:Microtubule-Associated Proteins; D011134:Polysaccharides; D011838:Radiation-Sensitizing Agents