title: Preferentially Expressed Antigen of Melanoma Prevents Lung Cancer Metastasis.
abstract: Lung cancer is the most common cause of cancer death worldwide. The poor survival rate is largely due to the extensive local invasion and metastasis. However, the mechanisms underlying the invasion and metastasis of lung cancer cells remain largely elusive. In this study, we examined the role of preferentially expressed antigen of melanoma (PRAME) in lung cancer metastasis. Our results show that PRAME is downregulated in lung adenocarcinoma and lung bone metastasis compared with normal human lung. Knockdown of PRAME decreases the expression of E-Cadherin and promotes the proliferation, invasion, and metastasis of lung cancer cells by regulating multiple critical genes, most of which are related to cell migration, including MMP1, CCL2, CTGF, and PLAU. Clinical data analysis reveals that the expression of MMP1 correlates with the clinical features and outcome of lung adenocarcinoma. Taken together, our data demonstrate that PRAME plays a role in preventing the invasion and metastasis of lung adenocarcinoma and novel diagnostic or therapeutic strategies can be developed by targeting PRAME.
authors.affiliation: Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, School of Life Sciences, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China.|Department of Orthopedic Oncology, Changzheng Hospital, The Second Military Medical University, No. 415 Fengyang Road, Shanghai, 200003, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Department of Orthopedic Oncology, Changzheng Hospital, The Second Military Medical University, No. 415 Fengyang Road, Shanghai, 200003, China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Wei
authors.affiliation: Department of Orthopedic Oncology, Changzheng Hospital, The Second Military Medical University, No. 415 Fengyang Road, Shanghai, 200003, China.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Department of Orthopedic Oncology, Changzheng Hospital, The Second Military Medical University, No. 415 Fengyang Road, Shanghai, 200003, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.affiliation: Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, School of Life Sciences, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China.
authors.forename: Liangfang
authors.initials: L
authors.lastname: Yao
authors.affiliation: Department of Orthopedic Oncology, Changzheng Hospital, The Second Military Medical University, No. 415 Fengyang Road, Shanghai, 200003, China.
authors.forename: Zhengwang
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences, School of Life Sciences, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China.|Department of Orthopedic Oncology, Changzheng Hospital, The Second Military Medical University, No. 415 Fengyang Road, Shanghai, 200003, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Orthopedic Oncology, Changzheng Hospital, The Second Military Medical University, No. 415 Fengyang Road, Shanghai, 200003, China.
authors.forename: Zaijun
authors.initials: Z
authors.lastname: Lin
authors.affiliation: Department of Orthopedic Oncology, Changzheng Hospital, The Second Military Medical University, No. 415 Fengyang Road, Shanghai, 200003, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.affiliation: Department of Orthopedic Oncology, Changzheng Hospital, The Second Military Medical University, No. 415 Fengyang Road, Shanghai, 200003, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Han
authors.affiliation: The International Peace Maternity& Child Health Hospital of China welfare institute (IPMCH), Shanghai, China.
authors.forename: Wenjiao
authors.initials: W
authors.lastname: Cao
authors.affiliation: Urology Department, Tenth People's Hospital of Tongji University, Shanghai, PR China.
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Orthopedic Oncology, Changzheng Hospital, The Second Military Medical University, No. 415 Fengyang Road, Shanghai, 200003, China.
authors.forename: Dianwen
authors.initials: D
authors.lastname: Song
authors.affiliation: Department of Orthopedic Oncology, Changzheng Hospital, The Second Military Medical University, No. 415 Fengyang Road, Shanghai, 200003, China.
authors.forename: Xinghai
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Orthopedic Oncology, Changzheng Hospital, The Second Military Medical University, No. 415 Fengyang Road, Shanghai, 200003, China.
authors.forename: Jianru
authors.initials: J
authors.lastname: Xiao
mesh_terms: D000072283:A549 Cells; D000230:Adenocarcinoma / Q000276:immunology* / Q000378:metabolism; D000077192:Adenocarcinoma of Lung; D000818:Animals; D015703:Antigens, CD; D000951:Antigens, Neoplasm / Q000378:metabolism*; D015820:Cadherins / Q000378:metabolism; D002465:Cell Movement; D049109:Cell Proliferation; D016000:Cluster Analysis; D018450:Disease Progression; D015536:Down-Regulation; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation
chemical_list: D015703:Antigens, CD; D000951:Antigens, Neoplasm; C513384:CDH1 protein, human; D015820:Cadherins; C404944:PRAME protein, human