title: High-Throughput Sequencing Reveals Immunological Characteristics of the TRB-/IgH-CDR3 Region of Umbilical Cord Blood.
abstract: To compare the differences of immunological characteristics between newborn and adults, we performed high-throughput sequencing to reveal the diversity of umbilical cord blood and adult peripheral blood at both T-cell receptor beta chain (TRB) and immunoglobulin heavy chain (IGH) levels.
  
  
  
  High-throughput sequencing was performed to analyze the expression of TRB-CDR3 and IGH-CDR3 in circulating T and B cells isolated from 20 healthy adults, 56 pregnant women, and 40 newborns.
  
  
  
  Our results revealed different immunological characteristics between newborn and adults, such as distinctive complementarity determining region 3 (CDR3) lengths, usage bias of variable and joining segments, random nucleotide addition, a large number of unique CDR3 peptides, and a greater repertoire diversity. Moreover, each newborn had a distinctive TRB-/IGH-CDR3 repertoire that was independent of the maternal immune status.
  
  
  
  This study presents comprehensive, unrestricted profiles of the TRB/IGH-CDR3 repertoire of newborns, pregnant women, and healthy adults at a sequence-level resolution. Our data may contribute to a better understanding of the immune system of newborns and benefit the efficient application of umbilical cord blood transplantation in future.
authors.affiliation: Department of Genetics, National Research Institute for Family Planning, Beijing, China.
authors.forename: Changlong
authors.initials: C
authors.lastname: Guo
authors.affiliation: Department of Genetics, National Research Institute for Family Planning, Beijing, China.
authors.forename: Qidi
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Genetics, National Research Institute for Family Planning, Beijing, China. Electronic address: mybrother1984@163.com.
authors.forename: Xiaofang
authors.initials: X
authors.lastname: Cao
authors.affiliation: Department of Genetics, National Research Institute for Family Planning, Beijing, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Genetics, National Research Institute for Family Planning, Beijing, China; Laboratory of Human Genetics, Beijing Hypertension League Institute, Beijing, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Genetics, National Research Institute for Family Planning, Beijing, China.
authors.forename: Lisha
authors.initials: L
authors.lastname: An
authors.affiliation: Department of Genetics, National Research Institute for Family Planning, Beijing, China.
authors.forename: Ruikun
authors.initials: R
authors.lastname: Cai
authors.affiliation: Department of Genetics, National Research Institute for Family Planning, Beijing, China; Graduate School of Peking Union Medical College, Beijing, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Du
authors.affiliation: Department of Genetics, National Research Institute for Family Planning, Beijing, China.
authors.forename: Guangyu
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Genetics, National Research Institute for Family Planning, Beijing, China; Graduate School of Peking Union Medical College, Beijing, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Qiu
authors.affiliation: Department of Genetics, National Research Institute for Family Planning, Beijing, China.
authors.forename: Zuoqi
authors.initials: Z
authors.lastname: Peng
authors.affiliation: HudsonAlpha Institute for Biotechnology, Huntsville, AL.
authors.forename: Jian
authors.initials: J
authors.lastname: Han
authors.affiliation: First Affiliated Hospital, Medical College of Shantou University, Shantou, Guangdong, China.
authors.forename: Shuhua
authors.initials: S
authors.lastname: Ni
authors.affiliation: First Affiliated Hospital, Medical College of Shantou University, Shantou, Guangdong, China.
authors.forename: Xuerui
authors.initials: X
authors.lastname: Tan
authors.affiliation: Beijing Friendship Hospital, Capital Medical University, Beijing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Jin
authors.affiliation: Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Healthy Care Hospital, Beijing, China.
authors.forename: Song
authors.initials: S
authors.lastname: Yu
authors.affiliation: Beijing Shijitan Hospital, Capital Medical University, Beijing, China.
authors.forename: Huiying
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Genetics, National Research Institute for Family Planning, Beijing, China; Stanford Genome Technology Center, Stanford University, Palo Alto, CA.
authors.forename: Chunlin
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Genetics, National Research Institute for Family Planning, Beijing, China; Laboratory of Human Genetics, Beijing Hypertension League Institute, Beijing, China.
authors.forename: Xingyu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Genetics, National Research Institute for Family Planning, Beijing, China; Graduate School of Peking Union Medical College, Beijing, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Ma
mesh_terms: D000328:Adult; D022801:Complementarity Determining Regions / Q000097:blood / Q000235:genetics* / Q000276:immunology*; D005260:Female; D005312:Fetal Blood; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000097:blood / Q000235:genetics* / Q000276:immunology*; D007231:Infant, Newborn; D011247:Pregnancy; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000097:blood / Q000235:genetics* / Q000276:immunology*; D017422:Sequence Analysis, DNA
chemical_list: D022801:Complementarity Determining Regions; D007143:Immunoglobulin Heavy Chains; D016693:Receptors, Antigen, T-Cell, alpha-beta
keywords: TRB-/IgH-CDR3 repertoires; high-throughput sequencing; umbilical cord blood