title: Evaluation of Culture Conditions to Obtain Fatty Acids from Saline Microalgae Species: Dunaliella salina, Sinecosyfis sp., and Chroomonas sp.
abstract: The use of the saline microalgae, Dunaliella salina, Sinecosyfis sp., and Chroomonas sp., was explored as an alternative source for the production of fatty acids using fertilizer and glycerol as culture media. The nutrient medium used contained "Nutrifoliar," a commercial fertilizer, and/or glycerol, in natural sea water. The microalgae were placed in cultures with different conditions. The parameters that favored the largest production of fatty acids were 24 hours of agitation and illumination, 1620 L/day of air supply, 2.25 L of air/min, and a temperature of 32°C using "Nutrifoliar" as the culture media. Results indicated that, from 3 g of microalgae in wet base of Chroomonas sp., 54.43 mg of oil was produced. The chromatographic characterization of oil obtained revealed the presence of essential fatty acids such as 9,12,15-octadecatrienoic acid (omega-3) and 4,7,10-hexadecatrienoic acid (omega-6) from the species Dunaliella salina. On the other hand, 9,12-octadecadienoic acid (omega-6) and cis-11-eicosenoic acid (omega-9) were identified from the species Chroomonas sp. The temperature variations played an important role in the velocity of growth or the production of the algae biomass, the amount of oil, and the ability to produce fatty acids.
authors.affiliation: Engineering Faculty, Chemical Engineering Program, Bioprocess Research Group, Universidad del Atlántico, Km 7 Antigua Vía a Puerto Colombia, Barranquilla, Colombia; Basic Sciences Faculty, Chemistry Program, Biotechnology of Microalgae Research Group, Universidad del Atlántico, Km 7 Antigua Vía a Puerto Colombia, Barranquilla, Colombia.
authors.forename: D A
authors.initials: DA
authors.lastname: Castilla Casadiego
authors.affiliation: Engineering Faculty, Chemical Engineering Program, Bioprocess Research Group, Universidad del Atlántico, Km 7 Antigua Vía a Puerto Colombia, Barranquilla, Colombia.
authors.forename: A R
authors.initials: AR
authors.lastname: Albis Arrieta
authors.affiliation: Basic Sciences Faculty, Chemistry Program, Biotechnology of Microalgae Research Group, Universidad del Atlántico, Km 7 Antigua Vía a Puerto Colombia, Barranquilla, Colombia.
authors.forename: E R
authors.initials: ER
authors.lastname: Angulo Mercado
authors.affiliation: Engineering Faculty, Chemical Engineering Program, Bioprocess Research Group, Universidad del Atlántico, Km 7 Antigua Vía a Puerto Colombia, Barranquilla, Colombia.
authors.forename: S J
authors.initials: SJ
authors.lastname: Cervera Cahuana
authors.affiliation: Engineering Faculty, Chemical Engineering Program, Bioprocess Research Group, Universidad del Atlántico, Km 7 Antigua Vía a Puerto Colombia, Barranquilla, Colombia.
authors.forename: K S
authors.initials: KS
authors.lastname: Baquero Noriega
authors.affiliation: Engineering Faculty, Universidad de Bogotá Jorge Tadeo Lozano, 4 Avenue No. 22-61, Bogotá, Colombia.
authors.forename: A F
authors.initials: AF
authors.lastname: Suárez Escobar
authors.affiliation: Sciences Experimental Faculty, Universidad del Zulia, Calle 61 con Prolongación Avenida 77, Maracaibo 4011, Venezuela.
authors.forename: E D
authors.initials: ED
authors.lastname: Morales Avendaño
mesh_terms: D005227:Fatty Acids / Q000378:metabolism*; D007700:Kinetics; D058086:Microalgae / Q000254:growth & development* / Q000378:metabolism*; D009821:Oils / Q000378:metabolism; D054712:Salinity; D013997:Time Factors
chemical_list: D005227:Fatty Acids; D009821:Oils