title: The effects of miR-1207-5p expression in peripheral blood on cisplatin-based chemosensitivity of primary gallbladder carcinoma.
abstract: OBJECTIVE
  The aim of this study was to investigate the association between miR-1207-5p expression in peripheral blood and the chemosensitivity of primary gallbladder carcinoma (PGBC).
  
  
  METHODS
  A total of 85 patients with PGBC undergoing preoperative chemotherapy were divided into effective (n=18) and ineffective (n=67) groups. Another 70 healthy individuals were selected as the control group. An miR-1207-5p mimic (mimic group), an inhibitor (inhibitor group), and a negative control (NC group) sequence were transfected into human gallbladder carcinoma GBC-SD cells. Real-time quantitative polymerase chain reaction was used to determine miR-1207-5p expression. After 48 hours of cisplatin treatment, CCK-8 method was used to detect cell proliferation and flow cytometry were performed to examine cell apoptosis.
  
  
  RESULTS
  miR-1207-5p expression in peripheral blood was significantly associated with tumor node metastasis staging of PGBC (P<0.05). Before chemotherapy, miR-1207-5p expression in patients was higher than in healthy individuals (P<0.05). After chemotherapy, the effective group had lower miR-1207-5p expression than the ineffective group (P<0.05). The rates of positive expression of Ki67 protein in the effective group were significantly lower than those in the ineffective group (P<0.05). Receiver operating characteristic curves showed that the area under curve, sensitivity, and specificity of miR-1207-5p used to diagnose PGBC were 0.898, 77.6%, and 97.1% at a cutoff of 1.470, respectively. After 48 hours of cisplatin treatment, compared with the NC group and nontransfected (non-T) group, the mimic group had decreased rates of cell inhibition and apoptosis, but the inhibitor group had increased rates (all P<0.05). The expression levels of caspase3 protein were increased in the mimic group and decreased in the inhibitor group. Cell survival rates in the mimic group at different time points after cisplatin treatment were significantly higher than the corresponding rates in the NC and non-T groups, whereas the cell survival rates in the inhibitor group were significantly lower than the rates in the NC and non-T groups (all P<0.05). The concentration and action time of cisplatin were negatively associated with the cell survival rate in each group (all P<0.05).
  
  
  CONCLUSIONS
  Cisplatin-based chemosensitivity of PGBC increased as expression of miR-1207-5p in peripheral blood declined. Thus, miR-1207-5p appears to be a promising and novel chemosensitizer for the treatment of PGBC.
authors.affiliation: Department of Oncology, The First People's Hospital of Yueyang, Yueyang.
authors.forename: Er-Dong
authors.initials: ED
authors.lastname: Shen
authors.affiliation: Department of General Surgery, The Second Xiangya Hospital of Central South University, Changsha.
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Radiotherapy, Qianfou Mount Hospital of Shandong Province, Jinan.
authors.forename: Xin-Shuang
authors.initials: XS
authors.lastname: Yu
authors.affiliation: Department of Radiotherapy, Ningbo Medical Treatment Center Lihuili Hospital, Ningbo.
authors.forename: Zhen-Fei
authors.initials: ZF
authors.lastname: Xiang
authors.affiliation: Department of Dermatology, The First People's Hospital of Yueyang, Yueyang, People's Republic of China.
authors.forename: Hui-Yun
authors.initials: HY
authors.lastname: Huang
keywords: chemosensitizer; cisplatin; diagnosis; miR-1207-5p; peripheral blood; primary gallbladder carcinoma