title: [Knockdown of STAT3 reduces the level of survivin and promotes the apoptosis of U87MG glioma cells].
abstract: Objective To investigate the effect of signal transducer and activator of transcription (STAT3)-specific siRNA (siSTAT3) on the apoptosis of U87MG human glioma cells and its mechanism. Methods U87MG glioma cells were cultured in vitro. The effect of siSTAT3 on U87MG human glioma cells was evaluated by MTT assay to observe cell proliferation and by flow cytometry and acridine orange staining to observe cell apoptosis. Reverse transcription PCR and Western blotting were used to detect the expression of survivin. Results Compared with mock and si-scrambled groups, siSTAT3 expression plasmid inhibited the proliferation of U87MG cells obviously. Flow cytometry and acridine orange staining showed that siSTAT3 plasmid significantly promoted glioma cell apoptosis. In addition, siSTAT3 plasmid significantly inhibited the mRNA and protein levels of survivin. Conclusion Knockdown of STAT3 in U87MG cells can downregulate the level of survivin and remarkably promote the apoptosis of glioma cells.
authors.affiliation: Department of Neurosurgery, Affiliated Hospital, Beihua University, Changchun 132000, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Pathophysiology, School of Basic Medical Sciences, Beihua University, Changchun 132000, China.
authors.forename: Yanbo
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Neurosurgery, Affiliated Hospital, Beihua University, Changchun 132000, China.
authors.forename: Xinli
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Neurosurgery, Affiliated Hospital, Beihua University, Changchun 132000, China. *Corresponding author, E-mail: shenweigao@163.com.
authors.forename: Weigao
authors.initials: W
authors.lastname: Shen
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D050796:STAT3 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D000077022:Survivin
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D012333:RNA, Messenger; D050796:STAT3 Transcription Factor; D000077022:Survivin