title: Usefulness of Aquaporin 1 as a Prognostic Marker in a Prospective Cohort of Malignant Mesotheliomas.
abstract: (1) BACKGROUND: Malignant mesothelioma (MM) is an aggressive tumour of the serosal membranes, associated with exposure to asbestos. Survival is generally poor, but prognostication for individual patients is difficult. We recently described Aquaporin 1 (AQP1) as independent prognostic factor in two separate retrospective cohorts of MM patients. Here we assess the usefulness of AQP1 prospectively, and determine the inter-observer agreement in assessing AQP1 scores; (2) METHODS: A total of 104 consecutive cases of MM were included. Sufficient tissue for immunohistochemistry was available for 100 cases, and these cases were labelled for AQP1. Labelling was assessed by two pathologists. Complete clinical information and follow up was available for 91 cases; (3) RESULTS: Labelling of ≥50% of tumour cells for AQP indicated improved prognosis in a univariate model (median survival 13 versus 8 months, p = 0.008), but the significance was decreased in a multivariate analysis. Scoring for AQP1 was robust, with an inter-observer kappa value of 0.722, indicating substantial agreement between observers; (4) CONCLUSION: AQP1 is a useful prognostic marker that can be easily incorporated in existing diagnostic immunohistochemical panels and which can be reliably interpreted by different pathologists.
authors.affiliation: Department of Anatomical Pathology, Flinders University of South Australia, Bedford Park, South Australia 5042, Australia. jack.driml@sa.gov.au.
authors.forename: Jack
authors.initials: J
authors.lastname: Driml
authors.affiliation: Department of Anatomical Pathology, Flinders University of South Australia, Bedford Park, South Australia 5042, Australia. pulf0010@flinders.edu.au.
authors.forename: Emily
authors.initials: E
authors.lastname: Pulford
authors.affiliation: Department of Surgical Pathology, SA Pathology at Flinders Medical Centre, Bedford Park, South Australia 5042, Australia. david.moffat@sa.gov.au.
authors.forename: David
authors.initials: D
authors.lastname: Moffat
authors.affiliation: Medical Oncology, Flinders Medical Centre, Bedford Park, South Australia 5042, Australia. chris.karapetis@sa.gov.au.
authors.forename: Christos
authors.initials: C
authors.lastname: Karapetis
authors.affiliation: Asbestos Diseases Research Institute, Bernie Banton Centre, Concord Repatriation General Hospital, Concord 2139 Sydney, Australia. Steven.Kao@lh.org.au.
authors.forename: Steven
authors.initials: S
authors.lastname: Kao
authors.affiliation: Department of Anatomical Pathology, Flinders University of South Australia, Bedford Park, South Australia 5042, Australia. kim.griggs2@sa.gov.au.
authors.forename: Kim
authors.initials: K
authors.lastname: Griggs
authors.affiliation: Department of Surgical Pathology, SA Pathology at Flinders Medical Centre, Bedford Park, South Australia 5042, Australia. dhenderson@internode.on.net.
authors.forename: Douglas Warrington
authors.initials: DW
authors.lastname: Henderson
authors.affiliation: Department of Anatomical Pathology, Flinders University of South Australia, Bedford Park, South Australia 5042, Australia. sonja.klebe@sa.gov.au.|Department of Surgical Pathology, SA Pathology at Flinders Medical Centre, Bedford Park, South Australia 5042, Australia. sonja.klebe@sa.gov.au.
authors.forename: Sonja
authors.initials: S
authors.lastname: Klebe
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D051398:Aquaporin 1 / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000401:mortality / Q000473:pathology; D008297:Male; D008654:Mesothelioma / Q000175:diagnosis* / Q000401:mortality / Q000473:pathology; D000086002:Mesothelioma, Malignant; D008875:Middle Aged; D015999:Multivariate Analysis; D011379:Prognosis; D016016:Proportional Hazards Models; D011446:Prospective Studies
chemical_list: D014408:Biomarkers, Tumor; D051398:Aquaporin 1
keywords: Aquaporin 1; asbestos; malignant mesothelioma; peritoneum; pleura; prognosis