title: HPLC Analysis of Anomeric Formation and Cleavage Pattern by Chitinolytic Enzyme.
abstract: The reactions of N-acetylchitooligosaccharides with chitinolytic enzyme were analyzed by HPLC using a Tosoh TSK-Gel amide-80 column with 70% acetonitrile as an eluent. We separated α and β anomeric forms of N-acetylchitooligosaccharides, and obtain the following advantages of this HPLC method. 1. We can easily identify the reaction mechanism of chitinolytic enzymes by this method, distinguishing the inverting mechanism showing α anomer formation from the retaining mechanism showing β anomer formation. 2. We can also estimate the cleavage patterns of N-acetylchitooligosaccharides by chitinolytic enzymes by using natural substrates.
authors.affiliation: a Laboratory of Biochemistry, Faculty of Agriculture, Yamaguchi University.
authors.forename: D
authors.initials: D
authors.lastname: Koga
authors.affiliation: a Laboratory of Biochemistry, Faculty of Agriculture, Yamaguchi University.
authors.forename: T
authors.initials: T
authors.lastname: Yoshioka
authors.affiliation: b Research Laboratory.
authors.forename: Y
authors.initials: Y
authors.lastname: Arakane
keywords: N-acetylchitooligosaccharide; chitinase; chitinolytic enzyme; lysozyme