title: Outbreak of hirame rhabdovirus infection in cultured spotted sea bass Lateolabrax maculatus on the western coast of Korea.
abstract: In this study, we determined the cause of a disease outbreak in spotted sea bass, Lateolabrax maculatus reared in culture cages on the western coast of Korea in 2013. The major signs in the diseased fish exhibited were haemorrhaging on the membranes of the abdomen, gastrointestinal organs and opercular gills, as well as an enlarged spleen. No external morphological signs of infection were visible, except for a darkening in colour. No parasites or pathological bacteria were isolated from the diseased fish; however, epithelioma papulosum cyprini (EPC) cells inoculated with tissue homogenates from the diseased fish showed cytopathic effects (CPEs). Virus particles in the EPC cells were bullet-shaped, 185-225 nm long and 70-80 nm wide, characteristic of Rhabdoviridae. Polymerase chain reaction analyses of homogenized tissues from the diseased fish and supernatants of cell cultures with CPEs indicated specific, 553-bp-long fragments corresponding to the matrix protein gene of the hirame rhabdovirus (HIRRV). Phylogenetically, the HIRRV phosphoprotein gene of spotted sea bass was more closely related to phosphoproteins from Chinese and Polish HIRRV strains than from other Korean strains. To our knowledge, this is the first report of HIRRV infection in cultured spotted sea bass.
authors.affiliation: Pathology Division, National Institute of Fisheries Science (NIFS), Busan, South Korea.
authors.forename: H-G
authors.initials: HG
authors.lastname: Seo
authors.affiliation: Pathology Division, National Institute of Fisheries Science (NIFS), Busan, South Korea.
authors.forename: J W
authors.initials: JW
authors.lastname: Do
authors.affiliation: Pathology Division, National Institute of Fisheries Science (NIFS), Busan, South Korea.
authors.forename: S H
authors.initials: SH
authors.lastname: Jung
authors.affiliation: Pathology Division, National Institute of Fisheries Science (NIFS), Busan, South Korea.
authors.forename: H-J
authors.initials: HJ
authors.lastname: Han
mesh_terms: D000818:Animals; D004196:Disease Outbreaks / Q000662:veterinary*; D005393:Fish Diseases / Q000453:epidemiology* / Q000821:virology; D029141:Novirhabdovirus / Q000235:genetics / Q000472:pathogenicity* / Q000502:physiology*; D010473:Perciformes; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D056910:Republic of Korea / Q000453:epidemiology; D018353:Rhabdoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D017422:Sequence Analysis, DNA / Q000662:veterinary; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D010750:Phosphoproteins; D014764:Viral Proteins
keywords: Korea; Lateolabrax maculates; hirame rhabdovirus; spotted sea bass