title: Gene expression pattern analysis of a recombinant Escherichia coli strain possessing high growth and lycopene production capability when using fructose as carbon source.
abstract: OBJECTIVE
  Escherichia coli K12f-pACLYC has a high capability for growth and lycopene production when using fructose as carbon source and the transcription of genes involved was compared in glucose-grown and fructose-grown cells.
  
  
  RESULTS
  Escherichia coli K12f-pACLYC was grown on 10 g fructose l(-1) and reached 4.6 g DCW l(-1) with lycopene at 192 mg g DCW(-1), values that are 3-fold and 7-fold higher than when growing on glucose. Gene transcription profiles of fructose-grown and glucose-grown cells were compared. 384 differentially expressed genes (DEGs) with fold changes ≥4 were identified, and the transcription of genes involved in fructose uptake and metabolism, pyruvate catabolism, tricarboxylic acid cycle and oxidative phosphorylation varied significantly. These changes enhanced the metabolic flux into the Embden-Meyerhof-Parnas pathway and the tricarboxylic acid cylcle and coupled to oxidative phosphorylation. These enhanced activities provide more precursors, cofactors and energy needed for growth lycopene production.
  
  
  CONCLUSIONS
  The high capability of E. coli K12f-pACLYC for growth and lycopene production when growing on fructose is due to transcriptional regulation, and the relevant genes were identified.
authors.affiliation: College of Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Street, Tianjin Economic and Technological Development Area, Tianjin, 300457, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Du
authors.affiliation: College of Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Street, Tianjin Economic and Technological Development Area, Tianjin, 300457, China.
authors.forename: Yajian
authors.initials: Y
authors.lastname: Song
authors.affiliation: College of Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Street, Tianjin Economic and Technological Development Area, Tianjin, 300457, China.
authors.forename: Mengya
authors.initials: M
authors.lastname: Liu
authors.affiliation: College of Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Street, Tianjin Economic and Technological Development Area, Tianjin, 300457, China.
authors.forename: Haixu
authors.initials: H
authors.lastname: Yang
authors.affiliation: College of Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Street, Tianjin Economic and Technological Development Area, Tianjin, 300457, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Street, Tianjin Economic and Technological Development Area, Tianjin, 300457, China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Fan
authors.affiliation: College of Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Street, Tianjin Economic and Technological Development Area, Tianjin, 300457, China.
authors.forename: Xuegang
authors.initials: X
authors.lastname: Luo
authors.affiliation: College of Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Street, Tianjin Economic and Technological Development Area, Tianjin, 300457, China.
authors.forename: Zhongyuan
authors.initials: Z
authors.lastname: Li
authors.affiliation: College of Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Street, Tianjin Economic and Technological Development Area, Tianjin, 300457, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.affiliation: College of Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Street, Tianjin Economic and Technological Development Area, Tianjin, 300457, China.
authors.forename: Hongpeng
authors.initials: H
authors.lastname: He
authors.affiliation: College of Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Street, Tianjin Economic and Technological Development Area, Tianjin, 300457, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhou
authors.affiliation: College of Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Street, Tianjin Economic and Technological Development Area, Tianjin, 300457, China.
authors.forename: Wenjian
authors.initials: W
authors.lastname: Ma
authors.affiliation: College of Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Street, Tianjin Economic and Technological Development Area, Tianjin, 300457, China. tony@tust.edu.cn.
authors.forename: Tongcun
authors.initials: T
authors.lastname: Zhang
mesh_terms: D002244:Carbon / Q000378:metabolism*; D002338:Carotenoids / Q000378:metabolism*; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D005632:Fructose / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D000077276:Lycopene
chemical_list: D005632:Fructose; D002338:Carotenoids; D002244:Carbon; D000077276:Lycopene
keywords: 2-C-Methyl-D-erythritol-4-phosphate pathway; Lycopene; Oxidative phosphorylation; Pyruvate; Transcriptome sequencing; Tricarboxylic acid cycle