title: Performance of MALDI biotyper compared with Vitek™2 compact system for fast identification and discrimination of Staphylococcus species isolated from bovine mastitis.
abstract: This study was designed to evaluate the ability of MALDI Biotyper (MBT) compared with Vitek™2 compact system for accurate identification of Staphylococcus aureus (S. aureus) and coagulase-negative staphylococci (CNS) strains and discriminate methicillin-sensitive S. aureus (MSSA) from methicillin-resistant S. aureus (MRSA). Throughout Al-Qassim region, Saudi Arabia, a total of 198 isolates of S. aureus (132 MSSA and 66 MRSA) and 44 CNS were collected from five dairy farms where the prevalence of staphylococcal mastitis was reported. The results produced by Vitek™2 compact system demonstrated that 123/132 MSSA isolates (93.18%), 61/66 MRSA (92.42%), and 37/44 CNS species (84.09%) were correctly identified. However; 130/132 MSSA (98.48%), 64/66 MRSA (96.96%), and 44/44 CNS (100%) were correctly identified by MBT with score ≥2. 00. The principal component analysis (PCA) dendrogram generated by MBT illustrated that the tested isolates were classified into two groups of Staphylococcus species at the distance level of 600. S. aureus isolates were found to be closely related with higher peak intensities in the mass of 3,993 Da, 4,121 Da and 5,845 Da were detected in MRSA, whereas, that were lost in MSSA.
  
  
  
  This study verified that MBT is an alternative powerful tool for precise identification and discrimination of Staphylococcus species.
authors.affiliation: Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Sadat City University, Sadat, Egypt.|Department of Public Health, Microbiology Unit, College of Public Health and Health Informatics, Qassim University, Buraidah, Saudi Arabia.
authors.forename: Ayman
authors.initials: A
authors.lastname: Elbehiry
authors.affiliation: Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Qassim University, Buraidah, Saudi Arabia.
authors.forename: Musaad
authors.initials: M
authors.lastname: Al-Dubaib
authors.affiliation: Department of Medical laboratories, College of Applied Medical Science, Qassim University, Buraidah, Saudi Arabia.
authors.forename: Eman
authors.initials: E
authors.lastname: Marzouk
authors.affiliation: Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Qassim University, Buraidah, Saudi Arabia.|Department of Animal Medicine, Faculty of Veterinary Medicine, Kafrelsheikh University, Buraidah, Egypt.
authors.forename: Salama
authors.initials: S
authors.lastname: Osman
authors.affiliation: Department of Physiology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
authors.forename: Husam
authors.initials: H
authors.lastname: Edrees
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods*; D002417:Cattle; D003124:Colorimetry / Q000379:methods; D003612:Dairying; D013058:Mass Spectrometry / Q000379:methods; D008414:Mastitis, Bovine / Q000175:diagnosis / Q000382:microbiology*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D025341:Principal Component Analysis; D040901:Proteomics / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: 
  MRSA
  ; 
  MSSA
  ; Discrimination; Identification; MALDI Biotyper; Staphylococcus species