title: Stability of Thermostable Enzyme, Aqualysin I ; a Subtilisin-type Serine Protease from Thermus aquaticus YT-1.
abstract: We characterized the heat stability and detergent stabilities of aqualysin I, produced by Thermus aquaticus YT-1, and compared them with those of fungal proteinase K and Bacillus subtilisin. Aqualysin I displayed excellent heat and detergent stabilities. Proteinase K, another Cys-containing enzyme, was less stable than aqualysin I. All these enzymes maintained activities in the presence of urea or Tween-20.
authors.affiliation: a Department of Biotechnology, The University of Tokyo.
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
authors.affiliation: a Department of Biotechnology, The University of Tokyo.
authors.forename: H
authors.initials: H
authors.lastname: Matsuzawa
authors.affiliation: a Department of Biotechnology, The University of Tokyo.
authors.forename: T
authors.initials: T
authors.lastname: Ohta
keywords: Thermus aquaticus YT-1; alkaline serine protease; detergents; subtilisin-type protease