title: Peptides from sesame cake extend healthspan of Caenorhabditis elegans via upregulation of skn-1 and inhibition of intracellular ROS levels.
abstract: The peptides from sesame cake (PSC) which are the main by-product of agricultural processing of sesame were prepared. To evaluate benefits of PSC for health and longevity, antioxidant activity and anti-aging effects were studied in vitro and in a Caenorhabditis elegans (C. elegans) model system. PSC exhibited antioxidant activity in vitro, and induced beneficial effects on lifespan and several health parameters of C.elegans, including pharyngeal pumping rate, locomotion and lipofuscin accumulation. In a mev-1 mutant, PSC increased lifespan, and it enhanced oxidative stress tolerance in wild-type nematodes. After treatment with PSC, SOD activity, GSH content, and GSH/GSSG ratio were increased, leading to low intracellular ROS levels in C. elegans. PSC up-regulated skn-1 mRNA, and its target gene gcs-1, and abolished the extension of lifespan in skn-1 mutant, indicating that PSC-mediated longevity is dependent on activation of the skn-1/Nrf-2 transcription factor. Current results warrant research into the use of PSC as nutraceuticals for overall health improvement.
authors.affiliation: School of Life Science, Shanxi University, Taiyuan 030006, Shanxi, PR China; Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, Shanxi, PR China. Electronic address: zhwang@sxu.edu.cn.
authors.forename: Zhuanhua
authors.initials: Z
authors.lastname: Wang
authors.affiliation: School of Life Science, Shanxi University, Taiyuan 030006, Shanxi, PR China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Ma
authors.affiliation: Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, Shanxi, PR China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, Shanxi, PR China. Electronic address: cuixiaodong@sxu.edu.cn.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Cui
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017173:Caenorhabditis elegans / Q000235:genetics / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D019587:Dietary Supplements; D008136:Longevity / Q000502:physiology*; D018384:Oxidative Stress / Q000187:drug effects; D010455:Peptides / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D031685:Sesamum / Q000737:chemistry*; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics*; D015854:Up-Regulation
chemical_list: D000975:Antioxidants; D029742:Caenorhabditis elegans Proteins; D004268:DNA-Binding Proteins; D010455:Peptides; D017382:Reactive Oxygen Species; D014157:Transcription Factors; C073702:skn-1 protein, C elegans
keywords: Antioxidant activity; Caenorhabditis elegans; Healthspan; Peptides from sesame cake; Skn-1