title: Salinicoccus amylolyticus sp. nov., isolated from a saltern.
abstract: A Gram-stain-positive coccus, strain JC304T, was isolated from a saltern of Nari along the Bhavnagar Coast, Gujarat, India. The 16S rRNA gene sequence analysis and sequence comparison data indicated that JC304T represented a member of the genus Salinicoccus and was most closely related to Salinicoccus roseus 9T (99.6 %), Salinicoccus luteus YIM 70202T (97.0 %), Salinicoccus hispanicus J-82T (97.0 %) and the remaining species of the genus Salinicoccus (<97 %). Genome relatedness based on DNA-DNA hybridization of JC304T with the type strains of the most closely related species was less than 46 % and the ΔTmwas >5 °C indicating that the strain represents a novel species of the genus Salinicoccus. Independent and concatenated phylogenetic analysis of recA/fusA gene translated product showed a clear distinction of JC304T from its phylogenetic neighbors. Diphosphotidylglycerol, phosphatidylglycerol, an unidentified glycolipid and three unidentified lipids (L1, L2 and L3) were the polar lipids of JC304T. Iso-C15 : 0 and anteiso-C15 : 0 were the major (>10 %) fatty acids in strain JC304T. The cell-wall amino acids were l-lysine and d-glycine. Hopanoids were not detected. The major isoprenoid quinone was menaquinone (MK-6). The DNA G+C content of JC304T was 48 mol%. On the basis of physiological, genotypic, phylogenetic and chemotaxonomic analyses, strain JC304T is considered to represent a novel species of the genus Salinicoccus, for which the name Salinicoccusamylolyticus sp. nov. is proposed. The type strain is JC304T (=KCTC 33661T=LMG 28757T).
authors.affiliation: Bacterial Discovery Laboratory, Center for Environment, Institute of Science and Technology, J. N. T. University Hyderabad, Kukatpally, Hyderabad 500085, India.
authors.forename: A
authors.initials: A
authors.lastname: Srinivas
authors.affiliation: Bacterial Discovery Laboratory, Center for Environment, Institute of Science and Technology, J. N. T. University Hyderabad, Kukatpally, Hyderabad 500085, India.
authors.forename: B
authors.initials: B
authors.lastname: Divyasree
authors.affiliation: Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P. O. Central University, Hyderabad 500046, India.
authors.forename: L
authors.initials: L
authors.lastname: Tushar
authors.affiliation: Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P. O. Central University, Hyderabad 500046, India.
authors.forename: G
authors.initials: G
authors.lastname: Suresh
authors.affiliation: Bacterial Discovery Laboratory, Center for Environment, Institute of Science and Technology, J. N. T. University Hyderabad, Kukatpally, Hyderabad 500085, India.
authors.forename: Ch
authors.initials: C
authors.lastname: Sasikala
authors.affiliation: Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P. O. Central University, Hyderabad 500046, India.
authors.forename: Ch V
authors.initials: CV
authors.lastname: Ramana
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D007194:India; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D017422:Sequence Analysis, DNA; D040141:Staphylococcaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D000596:Amino Acids; D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C042455:menaquinone 6