title: Metabolome Analysis of Oryza sativa (Rice) Using Liquid Chromatography-Mass Spectrometry for Characterizing Organ Specificity of Flavonoids with Anti-inflammatory and Anti-oxidant Activity.
abstract: Oryza sativa L. (rice) is an important staple crop across the world. In the previous study, we identified 36 specialized (secondary) metabolites including 28 flavonoids. In the present study, a metabolome analysis using liquid chromatography-mass spectrometry was conducted on the leaf, bran, and brown and polished rice grains to better understand the distribution of these metabolites. Principal component analysis using the metabolome data clearly characterized the accumulation patterns of the metabolites. Flavonoids, e.g., tricin, tricin 7-O-rutinoside, and tricin 7-O-β-D-glucopyranoside, were mainly present in the leaf and bran but not in the polished grain. In addition, anti-inflammatory and anti-oxidant activity of the metabolites were assayed in vitro. Tricin 4'-O-(erythro-β-guaiacylglyceryl)ether and isoscoparin 2″-O-(6‴-(E)-feruloyl)-glucopyranoside showed the strongest activity for inhibiting nitric oxide (NO) production and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging, respectively.
authors.affiliation: RIKEN Center for Sustainable Resource Science.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Yang
authors.forename: Ryo
authors.initials: R
authors.lastname: Nakabayashi
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Mori
authors.forename: Satoshi
authors.initials: S
authors.lastname: Takamatsu
authors.forename: Susumu
authors.initials: S
authors.lastname: Kitanaka
authors.forename: Kazuki
authors.initials: K
authors.lastname: Saito
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors* / Q000378:metabolism; D002853:Chromatography, Liquid; D005419:Flavonoids / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D005609:Free Radicals / Q000037:antagonists & inhibitors / Q000378:metabolism; D013058:Mass Spectrometry; D055442:Metabolome; D015394:Molecular Structure; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000378:metabolism; D012275:Oryza / Q000737:chemistry* / Q000378:metabolism; D010851:Picrates / Q000037:antagonists & inhibitors* / Q000378:metabolism
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000975:Antioxidants; D001713:Biphenyl Compounds; D005419:Flavonoids; D005609:Free Radicals; D010851:Picrates; D009569:Nitric Oxide; C004931:1,1-diphenyl-2-picrylhydrazyl