title: Differential Expressions of p53, p53R2, hRRM2 and PBR in Chronic Lymphocytic Leukemia: A Correlation with Intracellular Cholesterol.
abstract: Regulation of intracellular cholesterol homeostasis exists under balance between intracellular biosynthesis and uptake from extracellular origin by cell surface transport proteins. Expected role of cholesterol on either tumor suppressor gene and/or DNA synthesis has been aimed in the present study to explore intracellular cholesterol homeostasis in CLL subjects. Higher expressions of p53R2 (p53 dependent subunit of ribonucleotide reductase) and p53 were found in lymphocytes of chronic human lymphocytic leukemia as comparison to their normal counterparts. Inverse relation was found with p53 independent R2 subunit (in human hRRM2) of ribonucleotide reductase, which was found to be decreased from its control group. More expression of peripheral type benzodiazepine receptor, a cholesterol transporter, was noticed in isolated nuclear fraction with simultaneous increase of cholesterol concentration in cytoplasmic and nuclear compartments. A parallel increase of cholesterol in cell nucleus with increased p53R2 expression shows priority of the involvement of cholesterol in the process of cell replication.
authors.affiliation: All India Institute of Medical Sciences (AIIMS), Ansari Nagar, New Delhi, 110029 India ; CSIR-Institute of Genomics and Integrative Biology, Mathura Road, New Delhi, India.
authors.forename: Ankit
authors.initials: A
authors.lastname: Verma
authors.affiliation: All India Institute of Medical Sciences (AIIMS), Ansari Nagar, New Delhi, 110029 India ; All India Institute of Medical Sciences (AIIMS), Phulwari Sharif, Patna, Bihar 801507 India.
authors.forename: N C
authors.initials: NC
authors.lastname: Chandra
keywords: Chronic lymphocytic leukemia; hRRM2; p53; p53R2