title: Bacterial partition complexes segregate within the volume of the nucleoid.
abstract: Precise and rapid DNA segregation is required for proper inheritance of genetic material. In most bacteria and archaea, this process is assured by a broadly conserved mitotic-like apparatus in which a NTPase (ParA) displaces the partition complex. Competing observations and models imply starkly different 3D localization patterns of the components of the partition machinery during segregation. Here we use super-resolution microscopies to localize in 3D each component of the segregation apparatus with respect to the bacterial chromosome. We show that Par proteins locate within the nucleoid volume and reveal that proper volumetric localization and segregation of partition complexes requires ATPase and DNA-binding activities of ParA. Finally, we find that the localization patterns of the different components of the partition system highly correlate with dense chromosomal regions. We propose a new mechanism in which the nucleoid provides a scaffold to guide the proper segregation of partition complexes.
authors.affiliation: Centre de Biochimie Structurale, CNRS UMR5048, INSERM U1054, Université de Montpellier, 29 rue de Navacelles, 34090 Montpellier, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Le Gall
authors.affiliation: Centre de Biochimie Structurale, CNRS UMR5048, INSERM U1054, Université de Montpellier, 29 rue de Navacelles, 34090 Montpellier, France.
authors.forename: Diego I
authors.initials: DI
authors.lastname: Cattoni
authors.affiliation: Centre de Biochimie Structurale, CNRS UMR5048, INSERM U1054, Université de Montpellier, 29 rue de Navacelles, 34090 Montpellier, France.
authors.forename: Baptiste
authors.initials: B
authors.lastname: Guilhas
authors.affiliation: Laboratoire de Microbiologie et Génétique Moléculaires, Centre de Biologie Intégrative, Centre National de la Recherche Scientifique, Université de Toulouse, UPS, F-31000 Toulouse, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Mathieu-Demazière
authors.affiliation: Centre de Biochimie Structurale, CNRS UMR5048, INSERM U1054, Université de Montpellier, 29 rue de Navacelles, 34090 Montpellier, France.
authors.forename: Laura
authors.initials: L
authors.lastname: Oudjedi
authors.affiliation: Centre de Biochimie Structurale, CNRS UMR5048, INSERM U1054, Université de Montpellier, 29 rue de Navacelles, 34090 Montpellier, France.
authors.forename: Jean-Bernard
authors.initials: JB
authors.lastname: Fiche
authors.affiliation: Laboratoire de Microbiologie et Génétique Moléculaires, Centre de Biologie Intégrative, Centre National de la Recherche Scientifique, Université de Toulouse, UPS, F-31000 Toulouse, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Rech
authors.affiliation: HHMI, Lulu and Anthony Wang Laboratory of Neural Circuits and Behavior, The Rockefeller University, New York, New York 10065, USA.
authors.forename: Sara
authors.initials: S
authors.lastname: Abrahamsson
authors.affiliation: Centre for Bacterial Cell Biology, Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle Upon Tyne NE2 4AX, UK.
authors.forename: Heath
authors.identifier: 0000-0002-6467-3656
authors.initials: H
authors.lastname: Murray
authors.affiliation: Laboratoire de Microbiologie et Génétique Moléculaires, Centre de Biologie Intégrative, Centre National de la Recherche Scientifique, Université de Toulouse, UPS, F-31000 Toulouse, France.
authors.forename: Jean-Yves
authors.identifier: 0000-0003-1488-5455
authors.initials: JY
authors.lastname: Bouet
authors.affiliation: Centre de Biochimie Structurale, CNRS UMR5048, INSERM U1054, Université de Montpellier, 29 rue de Navacelles, 34090 Montpellier, France.
authors.forename: Marcelo
authors.identifier: 0000-0003-3339-2349
authors.initials: M
authors.lastname: Nollmann
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002451:Cell Compartmentation / Q000235:genetics; D020090:Chromosome Segregation; D002876:Chromosomes, Bacterial / Q000737:chemistry / Q000378:metabolism; D019915:DNA Primase / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D011485:Protein Binding
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; C052883:chromosome partition proteins, bacterial; D019915:DNA Primase; C121791:dnaG protein, E coli