title: MicroRNA-26a-interleukin (IL)-6-IL-17 axis regulates the development of non-alcoholic fatty liver disease in a murine model.
abstract: Non-alcoholic fatty liver disease (NAFLD) is a hepatic presentation of obesity and metabolic syndrome. MicroRNA 26a (Mir-26a) has been reported to play functions in cellular differentiation, cell growth, cell apoptosis and metastasis. A recent paper indicated that Mir-26a regulated insulin sensitivity and metabolism of glucose and lipids. However, the role of Mir-26a in NAFLD still needs to be investigated further. In our current study, vectors encoding pre-Mir-26a (LV-26a) and an empty lentiviral vector (LV-Con) delivered approximately 2 × 107transforming units of recombinant lentivirus were injected into mice through the tail vein. LV-26a-infected mice were protected from glucose dysmetabolism and showed markedly decreased total liver weight, hepatic triglyceride deposition and serum alanine transaminase (ALT) concentration when compared with LV-Con-treated mice. LV-26a-treated mice also exhibited decreased infiltration of immune cells in the liver - something attributed to reduce infiltration of T cell receptor (TCR)-γδ+, granulocyte-differentiation antigen-1 (Gr-1)+cells and CD11b+cells. Next, we found that Mir-26a inhibited the expression of interleukin (IL)-17 and IL-6 in vivo and in vitro. Furthermore, the decreased expression of IL-17 in the liver tissue induced by Mir-26a was abrogated completely by IL-6 overexpression. The decreased total liver weight, hepatic triglyceride deposition and serum ALT concentration induced by Mir-26a was also abrogated completely by IL-6 over-expression. In conclusion, the Mir-26a-IL-6-IL-17 axis regulates the development of NAFLD in a murine model.
authors.affiliation: Department of Infectious Disease, and Laboratory of Liver Disease, Renmin Hospital, Hubei University of Medicine, China.
authors.forename: Q
authors.initials: Q
authors.lastname: He
authors.affiliation: Department of Infectious Disease, and Laboratory of Liver Disease, Renmin Hospital, Hubei University of Medicine, China.
authors.forename: F
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Infectious Disease, and Laboratory of Liver Disease, Renmin Hospital, Hubei University of Medicine, China.
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Infectious Disease, and Laboratory of Liver Disease, Renmin Hospital, Hubei University of Medicine, China.
authors.forename: R
authors.initials: R
authors.lastname: Li
authors.affiliation: Department of Infectious Disease, and Laboratory of Liver Disease, Renmin Hospital, Hubei University of Medicine, China.
authors.forename: G
authors.initials: G
authors.lastname: Zhan
authors.affiliation: Department of Infectious Disease, and Laboratory of Liver Disease, Renmin Hospital, Hubei University of Medicine, China.
authors.forename: G
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Infectious Disease, and Laboratory of Liver Disease, Renmin Hospital, Hubei University of Medicine, China.
authors.forename: W
authors.initials: W
authors.lastname: Du
authors.affiliation: Department of Infectious Disease, and Laboratory of Liver Disease, Renmin Hospital, Hubei University of Medicine, China.
authors.forename: H
authors.initials: H
authors.lastname: Tan
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D004195:Disease Models, Animal; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D007333:Insulin Resistance / Q000235:genetics; D020381:Interleukin-17 / Q000235:genetics / Q000378:metabolism*; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism*; D016086:Lentivirus; D050356:Lipid Metabolism / Q000235:genetics; D008099:Liver / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics*; D065626:Non-alcoholic Fatty Liver Disease / Q000235:genetics* / Q000276:immunology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D020381:Interleukin-17; D015850:Interleukin-6; D035683:MicroRNAs; C543693:Mirn26 microRNA, mouse; D016692:Receptors, Antigen, T-Cell, gamma-delta; D000410:Alanine Transaminase
keywords: IL-17; IL-6; Mir-26a; NAFLD