title: Backbone resonance assignments for the SET domain of the human methyltransferase NSD2.
abstract: Aberrant NSD2 methyltransferase activity is implicated as the oncogenic driver in multiple myeloma, suggesting opportunities for novel therapeutic intervention. The methyltransferase activity of NSD2 resides in its catalytic SET domain, which is conserved among most lysine methyltransferases. Here we report the backbone [Formula: see text], N, C[Formula: see text], [Formula: see text] and side-chain [Formula: see text] assignments of a 25 kDa NSD2 SET domain construct, spanning residues 991-1203. A chemical shift analysis of C[Formula: see text], [Formula: see text] and [Formula: see text] resonances predicts a secondary structural pattern that is in agreement with homology models.
authors.affiliation: Discovery Sciences, Innovative Medicines and Early Development Biotech Unit, AstraZeneca, Alderley Park, SK10 4TG, UK.
authors.forename: Romel
authors.identifier: 0000-0001-5652-4098
authors.initials: R
authors.lastname: Bobby
authors.affiliation: Discovery Sciences, Innovative Medicines and Early Development Biotech Unit, AstraZeneca, Alderley Park, SK10 4TG, UK.
authors.forename: Karolina
authors.initials: K
authors.lastname: Peciak
authors.affiliation: Discovery Sciences, Innovative Medicines and Early Development Biotech Unit, AstraZeneca, Alderley Park, SK10 4TG, UK. Alex.Milbradt@astrazeneca.com.
authors.forename: Alexander G
authors.initials: AG
authors.lastname: Milbradt
mesh_terms: D011495:Histone-Lysine N-Methyltransferase / Q000737:chemistry*; D006801:Humans; D019906:Nuclear Magnetic Resonance, Biomolecular; D000072417:Protein Domains; D017433:Protein Structure, Secondary; D012097:Repressor Proteins / Q000737:chemistry*
chemical_list: D012097:Repressor Proteins; D011495:Histone-Lysine N-Methyltransferase; C419586:NSD2 protein, human
keywords: Backbone assignment; MMSET; NSD2; SET domain