title: The Post-genomic Era of Trichoderma reesei: What's Next?
abstract: The ascomycete Trichoderma reesei is one of the most well studied cellulolytic microorganisms. This fungus is widely used in the biotechnology industry, mainly in the production of biofuels. Due to its importance, its genome was sequenced in 2008, opening new avenues to study this microorganism. In this 'post-genomic' era, a transcriptomic and proteomic era has emerged. Here, we present an overview of new findings in the gene expression regulation network of T. reesei. We also discuss new rational strategies to obtain mutants that produce hydrolytic enzymes with a higher yield, using metabolic engineering. Finally, we present how synthetic biology strategies can be used to create engineered promoters to efficiently synthesize enzymes for biomass degradation to produce bioethanol.
authors.affiliation: Molecular Glyco-biotechnology Group, Discipline of Biochemistry, School of Natural Sciences, National University of Ireland Galway, Galway, Ireland. Electronic address: vijaifzd@gmail.com.
authors.forename: Vijai Kumar
authors.initials: VK
authors.lastname: Gupta
authors.affiliation: Departamento de Biologia Celular, Universidade de Brasília, Campus Universitário Darcy Ribeiro, Instituto de Ciências Biológicas, Brasília, DF, Brazil.
authors.forename: Andrei Stecca
authors.initials: AS
authors.lastname: Steindorff
authors.affiliation: Department of Biochemistry and Immunology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Renato Graciano
authors.initials: RG
authors.lastname: de Paula
authors.affiliation: Department of Cell and Molecular Biology-Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Rafael
authors.initials: R
authors.lastname: Silva-Rocha
authors.affiliation: Department for Biotechnology and Microbiology, Institute of Chemical Engineering, TU Wien, Wien, Austria.
authors.forename: Astrid R
authors.initials: AR
authors.lastname: Mach-Aigner
authors.affiliation: Department for Biotechnology and Microbiology, Institute of Chemical Engineering, TU Wien, Wien, Austria.
authors.forename: Robert L
authors.initials: RL
authors.lastname: Mach
authors.affiliation: Department of Biochemistry and Immunology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP, Brazil. Electronic address: rsilva@fmrp.usp.br.
authors.forename: Roberto N
authors.initials: RN
authors.lastname: Silva
mesh_terms: D056804:Biofuels; D019295:Computational Biology; D000431:Ethanol / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal / Q000235:genetics / Q000502:physiology; D016681:Genome, Fungal; D060847:Metabolic Engineering / Q000379:methods*; D020543:Proteome / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome / Q000235:genetics / Q000502:physiology; D014242:Trichoderma / Q000235:genetics / Q000378:metabolism
chemical_list: D056804:Biofuels; D020543:Proteome; D000431:Ethanol
keywords: Trichoderma reesei; biofuel; cellulases; synthetic biology; transcriptome