title: Knocking down of p53 triggers apoptosis and autophagy, concomitantly with inhibition of migration on SSC-4 oral squamous carcinoma cells.
abstract: Oral squamous cell carcinoma (OSCC) is a malignancy with elevated prevalence and somber prognosis due to the fact that most of the patients are diagnosed at an advanced stage. p53 has a crucial role in proliferation and apoptosis during the occurrence and development of numerous malignant tumors. The impact of mutated p53 on the development and progression of OSCC is unclear and might have therapeutic implications. Using an in vitro RNA interference experiment, we have evaluated the impact of p53 knockdown on cell viability, apoptosis, migration, and gene expression for key genes involved in apoptosis and angiogenesis. We observed that inhibiting the expression of p53 decreased the proliferation ability and induced apoptosis/autophagy in SSC-4 cells. Moreover, we observed that this has decreased migration and has blocked the expression of VEGF. In conclusion, our research provides a proof that a direct connection between p53 knockdown and OSCC cell death can be established, therefore opening new potential directions in OSCC molecular therapeutics and management.
authors.affiliation: Department of Dental Prosthetics and Dental Materials, Faculty of Dental Medicine, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
authors.forename: Alexandra Iulia
authors.initials: AI
authors.lastname: Irimie
authors.affiliation: Research Center for Functional Genomics, Biomedicine and Translational Medicine, "Iuliu Hatieganu" University of Medicine and Pharmacy, 23 Marinescu Street, 40015, Cluj-Napoca, Romania. braicucornelia@yahoo.com.
authors.forename: Cornelia
authors.initials: C
authors.lastname: Braicu
authors.affiliation: Research Center for Functional Genomics, Biomedicine and Translational Medicine, "Iuliu Hatieganu" University of Medicine and Pharmacy, 23 Marinescu Street, 40015, Cluj-Napoca, Romania.|Faculty of Pharmacy, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
authors.forename: Valentina
authors.initials: V
authors.lastname: Pileczki
authors.affiliation: Research Center for Functional Genomics, Biomedicine and Translational Medicine, "Iuliu Hatieganu" University of Medicine and Pharmacy, 23 Marinescu Street, 40015, Cluj-Napoca, Romania.|Laboratory of Radiobiology and Tumor Biology, The Oncology Institute "Prof. Dr. Ion Chiricuta", Cluj-Napoca, Romania.
authors.forename: Bobe
authors.initials: B
authors.lastname: Petrushev
authors.affiliation: Laboratory of Radiobiology and Tumor Biology, The Oncology Institute "Prof. Dr. Ion Chiricuta", Cluj-Napoca, Romania.
authors.forename: Olga
authors.initials: O
authors.lastname: Soritau
authors.affiliation: Department of Oral Rehabilitation, Faculty of Dental Medicine, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
authors.forename: Radu Septimiu
authors.initials: RS
authors.lastname: Campian
authors.affiliation: Research Center for Functional Genomics, Biomedicine and Translational Medicine, "Iuliu Hatieganu" University of Medicine and Pharmacy, 23 Marinescu Street, 40015, Cluj-Napoca, Romania. ioana.neagoe@umfcluj.ro.|Department of Functional Genomics and Experimental Pathology, The Oncology Institute "Prof. Dr. Ion Chiricuta", Cluj-Napoca, Romania. ioana.neagoe@umfcluj.ro.|MEDFUTURE -Research Center for Advanced Medicine, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania. ioana.neagoe@umfcluj.ro.
authors.forename: Ioana
authors.initials: I
authors.lastname: Berindan-Neagoe
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D001343:Autophagy / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002460:Cell Line; D002465:Cell Movement / Q000235:genetics*; D055785:Gene Knockdown Techniques; D006801:Humans; D009062:Mouth Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53
keywords: Apoptosis; Autophagy; Migration; Oral squamous cell carcinoma; SSC-4 cells; p53 siRNA