title: Continuous Hydrolysis of Pectate by Immobilized Endo-polygalacturonase in a Continuously Stirred Tank Reactor.
abstract: Enzymatic hydrolysis of pectate was carried out continuously to produce pectate oligosaccharides by immobilized endo-polygalacturonase in a continuous stirred tank reactor (CSTR) with high efficiency. The enzyme was immobilized on to chitosan beads by the absorption method, and the reaction was performed with an initial pectate concentration of 10 gl(-1) at 35°C and pH 4.0 at a dilution rate of 0.87-2.8 h(-1). The hydrolysis products mainly consisted of mono-, di-, tri-, tetra-, penta-, hexa- and heptasaccharides, with the highest conversion being 0.78. A higher volumetric production rate of the total hydrolyzate, which was dependent on the dilution rate, was obtained than that by a batch reaction. The hydrolysis process was mathematically modeled from the basic material balance and rate equations, and showed agreement between the simulated and experimental results. This reactor system was found to be effective for obtaining pectate oligosaccharides with a high production rate.
authors.affiliation: a Food Research Institute, Kagawa Prefectural Government.
authors.forename: K
authors.initials: K
authors.lastname: Iwasaki
authors.affiliation: a Food Research Institute, Kagawa Prefectural Government.
authors.forename: M
authors.initials: M
authors.lastname: Inoue
authors.affiliation: b Kagawa Prefectural Fermentation and Food Experimental Station.
authors.forename: Y
authors.initials: Y
authors.lastname: Matsubara
keywords: continuous hydrolysis; continuous stirred tank reactor (CSTR); oligosaccharide; pectin; polygalacturonase