title: Supercooling capacity and cryoprotectants of overwintering larvae from different populations of Holcocerus hippophaecolus.
abstract: BACKGROUND
  Holcocerus hippophaecolus is the most serious pest occurred in seabuckthorn forest of three north areas.
  
  
  OBJECTIVE
  The primary aims of the current study were to explore the physiological mechanisms and adaptability of H. hippophaecolus to low temperatures.
  
  
  METHODS
  Assessing supercooling point, freezing point, and cryoprotectants of different larval instars from three different populations.
  
  
  RESULTS
  Supercooling capacity of larvae from the 8-13 instar groups was relatively independent of temperature and other indicators such as latitude. Larvae from the 14-16 instar groups were sensitive to temperature and latitude, with generally lower limits and a wider range of SCPs than those of the other instar groups.
  
  
  CONCLUSIONS
  For each population, the differences in the supercooling capacity of different instar stages for the identical period were not significant. The metabolism of fat and glycogen might not be the primary factors affecting the supercooling capacity.
authors.forename: Bin
authors.initials: B
authors.lastname: Tian
authors.forename: Lili
authors.initials: L
authors.lastname: Xu
authors.forename: Miao
authors.initials: M
authors.lastname: Zhang
authors.forename: Yuqian
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing, China. zongsx@126.com.
authors.forename: Shixiang
authors.initials: S
authors.lastname: Zong
mesh_terms: D000064:Acclimatization; D000818:Animals; D001834:Body Water / Q000737:chemistry; D003080:Cold Temperature; D003451:Cryoprotective Agents / Q000032:analysis / Q000378:metabolism*; D007814:Larva / Q000254:growth & development / Q000502:physiology; D009036:Moths / Q000254:growth & development / Q000502:physiology*; D012621:Seasons
chemical_list: D003451:Cryoprotective Agents