title: MicroRNA-205-5p is upregulated in myelodysplastic syndromes and induces cell proliferation via PTEN suppression.
abstract: Micro (mi)RNA dysregulation is implicated in the development of myelodysplastic syndrome (MDS). Chromosomal abnormalities on 1q are frequently detected in Korean patients with MDS; however, how these are related to disease development is unknown. The present study compared the expression profiles of miRNAs encoded by chromosome 1q between 65 MDS patients and 11 controls. We found that miR-205-5p levels were 12.5 fold higher in the former (P=0.001). miR-205-5p level was increased in 44.7% of patients when an arbitrary 2(-ΔCt) cut-off value of 1.25 was used. miR-205-5p expression data were used to generate a receiver operating characteristic (ROC) curve for miR-205-5p, for which the area under the curve (AUC) was 0.825 (95% confidence interval: 0.710-0.941; P=0.001). Moreover, transfection with a miR-205-5p mimic induced cell proliferation by inhibiting the expression of the tumor suppressor protein phosphatase and tensin homolog (PTEN). Our findings suggest that miR-205-5p upregulation contributes to MDS by suppressing PTEN and that miR-205-5p thus acts as an oncogene in hematopoietic cells.
authors.affiliation: Department of Laboratory Medicine, Chosun University College of Medicine, Gwangju, Republic of Korea.
authors.forename: Sook Jin
authors.initials: SJ
authors.lastname: Jang
authors.affiliation: Department of Laboratory Medicine, Chosun University College of Medicine, Gwangju, Republic of Korea.
authors.forename: In-Sun
authors.initials: IS
authors.lastname: Choi
authors.affiliation: Department of Laboratory Medicine, Chosun University College of Medicine, Gwangju, Republic of Korea.
authors.forename: Geon
authors.initials: G
authors.lastname: Park
authors.affiliation: Department of Laboratory Medicine, Chosun University College of Medicine, Gwangju, Republic of Korea.
authors.forename: Dae-Soo
authors.initials: DS
authors.lastname: Moon
authors.affiliation: Department of Laboratory Medicine, Korea University College of Medicine, Seoul, Republic of Korea.
authors.forename: Ji-Seon
authors.initials: JS
authors.lastname: Choi
authors.affiliation: Department of Laboratory Medicine, Korea University College of Medicine, Seoul, Republic of Korea.
authors.forename: Myung-Hyun
authors.initials: MH
authors.lastname: Nam
authors.affiliation: Department of Laboratory Medicine, Korea University College of Medicine, Seoul, Republic of Korea.
authors.forename: Soo-Young
authors.initials: SY
authors.lastname: Yoon
authors.affiliation: Department of Pharmacology, Chosun University College of Medicine, Gwangju, Republic of Korea.
authors.forename: Cheol Hee
authors.initials: CH
authors.lastname: Choi
authors.affiliation: Department of Laboratory Medicine, Chosun University College of Medicine, Gwangju, Republic of Korea. Electronic address: seonghomed@hanmail.net.
authors.forename: Seong-Ho
authors.initials: SH
authors.lastname: Kang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D002460:Cell Line; D049109:Cell Proliferation; D002869:Chromosome Aberrations; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007621:Karyotyping; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics* / Q000473:pathology; D051059:PTEN Phosphohydrolase / Q000037:antagonists & inhibitors; D012372:ROC Curve; D056910:Republic of Korea; D015854:Up-Regulation; D055815:Young Adult
chemical_list: D035683:MicroRNAs; D051059:PTEN Phosphohydrolase; C534425:MIR205, human; C494929:PTEN protein, human
keywords: Diagnosis; MDS; Oncogene; Upregulation; miR-205-5p