title: Bacterial Population in Intestines ofLitopenaeus vannameiFed Different Probiotics or Probiotic Supernatant.
abstract: The interactions of microbiota in the gut play an important role in promoting or maintaining the health of hosts. In this study, in order to investigate and compare the effects of dietary supplementation withLactobacillus pentosusHC-2 (HC-2),Enterococcus faeciumNRW-2, or the bacteria-free supernatant of a HC-2 culture on the bacterial composition ofLitopenaeus vannamei, Illumina sequencing of the V1-V2 region of the 16S rRNA gene was used. The results showed that unique species exclusively existed in specific dietary groups, and the abundance of Actinobacteria was significantly increased in the intestinal bacterial community of shrimp fed with the bacteria-free supernatant of an HC-2 culture compared with the control. In addition, the histology of intestines of the shrimp from the four dietary groups was also described, but no obvious improvements in the intestinal histology were observed. The findings in this work will help to promote the understanding of the roles of intestinal bacteria in shrimps when fed with probiotics or probiotic supernatant.
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, P.R. China.|Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, P.R. China.|University of Chinese Academy of Sciences, Beijing 100049, P.R. China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Sha
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, P.R. China.|Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, P.R. China.
authors.forename: Mei
authors.initials: M
authors.lastname: Liu
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, P.R. China.|Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, P.R. China.
authors.forename: Baojie
authors.initials: B
authors.lastname: Wang
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, P.R. China.|Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, P.R. China.
authors.forename: Keyong
authors.initials: K
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, P.R. China.|Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, P.R. China.|University of Chinese Academy of Sciences, Beijing 100049, P.R. China.
authors.forename: Cancan
authors.initials: C
authors.lastname: Qi
authors.affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, P.R. China.|Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics; D000069196:Gastrointestinal Microbiome / Q000235:genetics* / Q000502:physiology*; D007422:Intestines / Q000382:microbiology*; D033561:Penaeidae / Q000382:microbiology*; D019936:Probiotics
keywords: Litopenaeus vannamei; Probiotics; bacterial composition; histology; supernatant