title: Parallel temperature-dependent microrheological measurements in a microfluidic chip.
abstract: Microfluidic stickers are used as a sample environment to measure the microrheology of monoclonal antibody (mAb) protein solutions. A Peltier-based microscope stage is implemented and validated, and is capable of controlling the sample temperature over the range 0.9-40 °C. The design accounts for heat transfer to and from the objective, controls the sample environment humidity to mitigate condensation, and provides adequate damping to reduce vibration from the cooling system. A concentrated sucrose solution is used as a standard sample to provide an in situ temperature measurement by the Stokes-Einstein-Sutherland relation. By combining microfluidic stickers and microrheology, 72 temperature-concentration viscosity measurements of mAb solutions can be made in 1 day, a significant increase in throughput over conventional rheometry.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Delaware , Newark, Delaware 19716, USA.
authors.forename: Lilian Lam
authors.identifier: 0000-0003-2560-3548
authors.initials: LL
authors.lastname: Josephson
authors.affiliation: Early Stage Pharmaceutical Development, Genentech, Inc. , South San Francisco, California 94080, USA.
authors.forename: William J
authors.initials: WJ
authors.lastname: Galush
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Delaware , Newark, Delaware 19716, USA.
authors.forename: Eric M
authors.initials: EM
authors.lastname: Furst