title: Extremely High Expression of Antisense RNA for Wilms' Tumor 1 in Active Osteoclasts: Suppression of Wilms' Tumor 1 Protein Expression during Osteoclastogenesis.
abstract: Wilms' tumor 1 (WT1), a zinc-finger transcription regulator of the early growth response family, identified as the product of a tumor suppressor gene of Wilms' tumors, bears potential ability to induce macrophage differentiation in blood cell differentiation. Herein, we examined the involvement of WT1 in the regulation of osteoclastogenesis. We detected a high level of WT1 protein expression in osteoclast precursors; however, WT1 expression was markedly suppressed during osteoclastogenesis. We examined expression of WT1 transcripts in bone tissue by RNA in situ hybridization. We found a high level of antisense transcripts in osteoclasts actively resorbing bone in mandible of newborn rats. Expression of antisense WT1 RNA in mandible was also confirmed by Northern blot analysis and strand-specific RT-PCR. Overexpression of antisense WT1 RNA in RAW-D cells, an osteoclast precursor cell line, resulted in a marked enhancement of osteoclastogenesis, suggesting that antisense WT1 RNA functions to suppress expression of WT1 protein in osteoclastogenesis. High level expression of antisense WT1 RNA may contribute to commitment to osteoclastogenesis, and may allow osteoclasts to maintain or stabilize their differentiation state.
authors.affiliation: Division of Molecular Cell Biology and Oral Anatomy, Division of Oral Biological Sciences, Faculty of Dental Science, Kyushu University, Fukuoka, Japan.
authors.forename: Yin-Ji
authors.initials: YJ
authors.lastname: Li
authors.affiliation: Department of Microbiology, Faculty of Medicine, Saga University, Saga, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Kukita
authors.affiliation: Division of Molecular Cell Biology and Oral Anatomy, Division of Oral Biological Sciences, Faculty of Dental Science, Kyushu University, Fukuoka, Japan.
authors.forename: Yukari
authors.initials: Y
authors.lastname: Kyumoto-Nakamura
authors.affiliation: Division of Molecular Cell Biology and Oral Anatomy, Division of Oral Biological Sciences, Faculty of Dental Science, Kyushu University, Fukuoka, Japan. Electronic address: kukitat@dent.kyushu-u.ac.jp.
authors.forename: Toshio
authors.initials: T
authors.lastname: Kukita
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002454:Cell Differentiation / Q000235:genetics*; D002460:Cell Line; D005786:Gene Expression Regulation; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D010010:Osteoclasts / Q000166:cytology*; D010012:Osteogenesis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D016372:RNA, Antisense / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D025721:WT1 Proteins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D016372:RNA, Antisense; D025721:WT1 Proteins