title: The Nuclear DNA Content and Genetic Diversity of Lampetra morii.
abstract: We investigated the nuclear DNA content and genetic diversity of a river lamprey, the Korean lamprey Lampetra morii, which is distributed in the northeast of China. L. morii spends its whole life cycle in fresh water, and its adult size is relatively small (~160 mm long) compared with that of other lampreys. The haploid nuclear DNA content of L. morii is 1.618 pg (approximately 1.582 Gb) in germline cells, and there is ~15% germline DNA loss in somatic cells. These values are significantly smaller than those of Petromyzon marinus, a lamprey with a published draft genome. The chromosomes of L. morii are small and acrocentric, with a diploid modal number of 2n = 132, lower than some other lampreys. Sequence and AFLP analyses suggest that the allelic polymorphism rate (~0.14% based on examined nuclear and mitochondrial DNA sequences) of L. morii is much lower than that (~2%) of P. marinus. Phylogenetic analysis based on a mitochondrial DNA fragment confirms that L. morii belongs to the genus Lampetra, which, together with the genus Lethenteron, forms a sister group to P. marinus. These genetic background data are valuable for subsequent genetic and genomic research on L. morii.
authors.affiliation: State Key Laboratory of Biocontrol, Guangdong Key Laboratory of Pharmaceutical Functional Genes, School of Life Sciences, Sun Yat-sen University, Guangzhou, People's Republic of China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Yan
authors.affiliation: State Key Laboratory of Biocontrol, Guangdong Key Laboratory of Pharmaceutical Functional Genes, School of Life Sciences, Sun Yat-sen University, Guangzhou, People's Republic of China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Meng
authors.affiliation: State Key Laboratory of Biocontrol, Guangdong Key Laboratory of Pharmaceutical Functional Genes, School of Life Sciences, Sun Yat-sen University, Guangzhou, People's Republic of China.
authors.forename: Fenfang
authors.initials: F
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Biocontrol, Guangdong Key Laboratory of Pharmaceutical Functional Genes, School of Life Sciences, Sun Yat-sen University, Guangzhou, People's Republic of China.|Beijing University of Chinese Medicine, Beijing, People's Republic of China.
authors.forename: Anlong
authors.initials: A
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Biocontrol, Guangdong Key Laboratory of Pharmaceutical Functional Genes, School of Life Sciences, Sun Yat-sen University, Guangzhou, People's Republic of China.
authors.forename: Shangwu
authors.initials: S
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Biocontrol, Guangdong Key Laboratory of Pharmaceutical Functional Genes, School of Life Sciences, Sun Yat-sen University, Guangzhou, People's Republic of China.
authors.forename: Shengfeng
authors.initials: S
authors.lastname: Huang
mesh_terms: D000483:Alleles; D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D002467:Cell Nucleus / Q000235:genetics*; D002681:China; D002875:Chromosomes; D004247:DNA / Q000235:genetics*; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics*; D014644:Genetic Variation; D007621:Karyotyping; D007798:Lampreys / Q000235:genetics*; D008018:Life Cycle Stages; D010802:Phylogeny; D011003:Ploidies; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial; D004247:DNA