title: Microdialysis combined with UPLC-MS/MS method for determination of tetramethylpyrazine and ferulic acid in striatum of awake and anesthetic rats subjected to cerebral ischemia.
abstract: A rapid, sensitive and selective ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method has been developed for the simultaneous determination and pharmacokinetic investigation of Tetramethylpyrazine (TMP) and Ferulic acid (FA) in rat striatum. The method was validated over the concentration range of 1.15-505ng/mL for TMP and 3.23-101ng/mL for FA, with a lower limit of quantitation (LLOQ) of 1.15ng/mL and 3.23ng/mL, respectively. This method can be successfully applied in pharmacokinetic studies of TMP and FA in striatum of awake and anesthetic rats. The cerebral blood flow velocity (CBF) during middle cerebral artery occlusion (MCAO) was monitored by Laser speckle contrast imaging, to observe whether the compatibility of TMP and FA could improve CBF against cerebral ischemia/reperfusion (I/R) injury. Infarct volume was examined to evaluate severity of ischemic brain injury. The pharmacokinetic study indicated that T1/2, Cmax, MRT and AUC0-inf were changed after combined administration of TMP and FA, when compared with either drug alone both in awake and anesthetic groups. The pharmacodynamics results showed that co-administration of drugs could enhance the CBF during middle cerebral artery occlusion and reduced the infarct volume. Taken together, the compatibility treatment of TMP and FA might be a promising therapeutic strategy for ischemic stroke. Further study is required to optimize the compatibility proportion.
authors.affiliation: Department of Pharmacy, College of Chinese Traditional Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
authors.forename: Weiguo
authors.initials: W
authors.lastname: Liao
authors.affiliation: Department of Pharmacy, College of Chinese Traditional Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
authors.forename: Jianye
authors.initials: J
authors.lastname: Yu
authors.affiliation: Department of Pharmacy, College of Chinese Traditional Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
authors.forename: Zhonglin
authors.initials: Z
authors.lastname: Guo
authors.affiliation: Department of Pharmacy, College of Chinese Traditional Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
authors.forename: Wenqiang
authors.initials: W
authors.lastname: Ba
authors.affiliation: Department of Pharmacy, College of Chinese Traditional Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
authors.forename: Ding
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Pharmacy, College of Chinese Traditional Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Pharmacy, College of Chinese Traditional Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
authors.forename: Wentao
authors.initials: W
authors.lastname: Fan
authors.affiliation: Department of Pharmacy, College of Chinese Traditional Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
authors.forename: Fengyun
authors.initials: F
authors.lastname: Liao
authors.affiliation: Department of Pharmacy, College of Chinese Traditional Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
authors.forename: Yinai
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Pharmacy, College of Chinese Traditional Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China. Electronic address: wlis68@sina.com.
authors.forename: Lisheng
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D002560:Cerebrovascular Circulation / Q000502:physiology; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003342:Corpus Striatum / Q000378:metabolism*; D003373:Coumaric Acids / Q000493:pharmacokinetics* / Q000494:pharmacology; D004357:Drug Synergism; D057230:Limit of Detection; D008297:Male; D017551:Microdialysis / Q000379:methods*; D011719:Pyrazines / Q000493:pharmacokinetics* / Q000494:pharmacology; D051381:Rats; D015427:Reperfusion Injury / Q000378:metabolism* / Q000503:physiopathology / Q000517:prevention & control; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D003373:Coumaric Acids; D011719:Pyrazines; C004999:ferulic acid; C017953:tetramethylpyrazine
keywords: Ferulic acid; Laser speckle contrast imaging; Microdialysis; Pharmacokinetics; Tetramethylpyrazine; UPLC–MS/MS