title: Charaterization and immunomodulatory activities of polysaccharide isolated from Pleurotus eryngii.
abstract: A water-soluble polysaccharide (EPA-1) from Pleurotus eryngii was obtained using DEAE-52 and Sephadex G-50 columns. The properties, structure and immunomodulatory activity of EPA-1 were studied. The results demonstrated that EPA-1 was a homogeneous polysaccharide with the molecular weight of 9.97×104Da. EPA-1 consisted of Man, Glc and Gal in a molar ratio of 2.2:1.0:3.2. The characterized fragment structures of EPA-1 were found to be consisting of seven sugar residues and two branches by GC-MS, FTIR and NMR analyses. Among them, the (1→6)-linkedGal residue was the main linkage mode of EpA-1 and composed of its backbone. Activity tests indicated that EPA-1 significantly induced macrophage to release the immune activity factor of NO, TNF-α, IL-1 and IL-6 through activation of signal protein of p38, ERK, JNK in MAPKs and translocation of nuclear NF-κΒ, indicating EPA-1 to possess good immunoregulatory activity.
authors.affiliation: Center of Research & Development, Changchun University of Chinese Medicine, Changchun, Jilin 130117, China; Macromolecule of Chinese Medicine Key Lab of Jilin Province, Changchun, Jilin 130117, China.
authors.forename: Duoduo
authors.initials: D
authors.lastname: Xu
authors.affiliation: Center of Research & Development, Changchun University of Chinese Medicine, Changchun, Jilin 130117, China; Macromolecule of Chinese Medicine Key Lab of Jilin Province, Changchun, Jilin 130117, China.
authors.forename: Haiyang
authors.initials: H
authors.lastname: Wang
authors.affiliation: Departments of Ophthalmology, the Affiliated Hospital, Changchun University of Chinese Medicine, Changchun, Jilin 130021, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zheng
authors.affiliation: Center of Research & Development, Changchun University of Chinese Medicine, Changchun, Jilin 130117, China; Macromolecule of Chinese Medicine Key Lab of Jilin Province, Changchun, Jilin 130117, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Macromolecule of Chinese Medicine Key Lab of Jilin Province, Changchun, Jilin 130117, China; Preparation Center, the Affiliated Hospital, Changchun University of Chinese Medicine, Changchun, Jilin 130021, China.
authors.forename: Mingxing
authors.initials: M
authors.lastname: Wang
authors.affiliation: Preparation Center, the Affiliated Hospital, Changchun University of Chinese Medicine, Changchun, Jilin 130021, China.
authors.forename: Yanqiu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Center of Research & Development, Changchun University of Chinese Medicine, Changchun, Jilin 130117, China; Macromolecule of Chinese Medicine Key Lab of Jilin Province, Changchun, Jilin 130117, China. Electronic address: czxuduoduo@163.com.
authors.forename: Qipin
authors.initials: Q
authors.lastname: Gao
mesh_terms: D000818:Animals; D002236:Carbohydrate Conformation; D045744:Cell Line, Tumor; D062610:Fungal Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D007155:Immunologic Factors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D020935:MAP Kinase Signaling System / Q000187:drug effects* / Q000276:immunology; D008264:Macrophages / Q000276:immunology / Q000378:metabolism*; D008297:Male; D051379:Mice; D015846:Monokines / Q000097:blood / Q000276:immunology; D009569:Nitric Oxide / Q000276:immunology / Q000378:metabolism; D020076:Pleurotus / Q000737:chemistry*
chemical_list: D062610:Fungal Polysaccharides; D007155:Immunologic Factors; D015846:Monokines; D009569:Nitric Oxide
keywords: Cytokine; Immunostimulation; MAPKs; NF-κB; Pleurotus eryngii; Polysaccharide