title: Novel phenanthridine (PHE-4i) derivative inhibits carrageenan-induced rat hind paw oedema through suppression of hydrogen sulfide.
abstract: This study was conducted to assess the anti-inflammatory effect of a novel synthesized phenanthridine alkaloid (PHE-4i) and to examine the possible involvement of hydrogen sulfide (H2S) in anti-inflammatory mechanism. The synthesized phenanthridine derivative PHE-4i (2, 5, and 10 mg/kg) was administered intraperitoneally to rats. One hour following treatment, inflammation was induced by intraplantar injection of carrageenan (1 %), in the hind paw. Paw volume as the index of inflammation was measured before and after carrageenan injection. Neutrophil sequestration into the hind paw was quantified by measuring tissue myeloperoxidase (MPO) activity and was compared for the inhibition of H2S production. Pretreatment with PHE-4i significantly reduced carrageenan-induced hind paw weight, MPO activity, leukocyte infiltration, and H2S production in a dose-dependent manner (p < 0.001). These results indicate that the anti-inflammatory effect of PHE-4i on carrageenan-induced rat paw oedema could be via the inhibition of the gaseous mediator H2S.
authors.affiliation: School of Bio-Sciences and Technology, Vellore Institute of Technology, Vellore, 632 014, Tamilnadu, India.
authors.forename: Leema
authors.initials: L
authors.lastname: George
authors.affiliation: School of Bio-Sciences and Technology, Vellore Institute of Technology, Vellore, 632 014, Tamilnadu, India. tamizhselvi.r@vit.ac.in.
authors.forename: Tamizhselvi
authors.initials: T
authors.lastname: Ramasamy
authors.affiliation: School of Bio-Sciences and Technology, Vellore Institute of Technology, Vellore, 632 014, Tamilnadu, India.
authors.forename: Venkatraman
authors.initials: V
authors.lastname: Manickam
authors.affiliation: School of Advanced Sciences, Vellore Institute of Technology, Vellore, 632 014, Tamilnadu, India.
authors.forename: Sathiyanarayanan Kulathu
authors.initials: SK
authors.lastname: Iyer
authors.affiliation: School of Bio-Sciences and Technology, Vellore Institute of Technology, Vellore, 632 014, Tamilnadu, India.
authors.forename: Vidya
authors.initials: V
authors.lastname: Radhakrishnan
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D002351:Carrageenan / Q000008:administration & dosage*; D004195:Disease Models, Animal; D004487:Edema / Q000188:drug therapy* / Q000378:metabolism; D006862:Hydrogen Sulfide / Q000037:antagonists & inhibitors*; D008297:Male; D015394:Molecular Structure; D020556:Neutrophil Infiltration / Q000187:drug effects; D009195:Peroxidase / Q000037:antagonists & inhibitors; D010617:Phenanthridines / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D017208:Rats, Wistar
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D010617:Phenanthridines; D002351:Carrageenan; D009195:Peroxidase; D006862:Hydrogen Sulfide
keywords: Carrageenan; Hind paw oedema; Hydrogen sulfide; Myeloperoxidase; Phenanthridine alkaloid