title: Evaluation of a DNA microarray for rapid detection of the most prevalent extended-spectrum β-lactamases, plasmid-mediated cephalosporinases and carbapenemases in Enterobacteriaceae, Pseudomonas and Acinetobacter.
abstract: The dissemination of Gram-negative bacteria (GNB) producing extended-spectrum β-lactamases (ESBLs), plasmid-encoded cephalosporinases (pAmpCs) and carbapenemases is a matter of great clinical concern. In this study, we evaluated a new low-density DNA array 'Check-MDR CT103 XL' (Check-Points, Wageningen, The Netherlands) that identifies the most clinically relevant β-lactamase genes of ESBLs (blaTEM, blaSHV, blaCTX-M, blaBEL, blaPER, blaGES and blaVEB), pAmpCs (blaCMY-2-like, blaDHA, blaFOX, blaACC-1, blaACT/MIR and blaCMY-1-like/MOX) and carbapenemases (blaKPC, blaOXA-48, blaVIM, blaIMP, blaNDM, blaGIM, blaSPM and blaOXA-23, -24 and -58) in cultured bacteria. In total, 223 GNB isolates with well-characterised resistance mechanisms to β-lactams were analysed. A specificity and sensitivity of 100% were recorded for most bla genes, with a slightly lower signal observed for blaIMP. The Check-MDR CT103 XL array proved highly accurate for the identification of epidemiologically relevant ESBL, pAmpC and carbapenemase genes harboured in Enterobacteriaceae, Pseudomonas and Acinetobacter spp. The Check-MDR CT103 XL assay is a significant improvement compared with Check-MDR CT103 and it highlights the ability of this array to evolve rapidly to adjust to the current needs for the detection of resistance mechanisms to β-lactam agents.
authors.affiliation: Laboratoire de Bactériologie, Centre national de référence des Enterobactéries, Pseudomonas et Acinetobacter multirésistant aux antibiotiques, CHU UCL Namur, 1 Avenue G. Thérasse, 5530 Yvoir, Belgium. Electronic address: pierre.bogaerts@uclouvain.be.
authors.forename: Pierre
authors.initials: P
authors.lastname: Bogaerts
authors.affiliation: Service de Bactériologie-Virologie, CNR associé de la résistance aux antibiotiques 'Entérobactéries productrices de carbapénèmases', Hôpital de Bicêtre, Faculté de Médecine Paris-Sud, Assistance Publique-Hôpitaux de Paris, Le Kremlin-Bicêtre, France.
authors.forename: Gaelle
authors.initials: G
authors.lastname: Cuzon
authors.affiliation: Laboratoire de Bactériologie, Centre national de référence des Enterobactéries, Pseudomonas et Acinetobacter multirésistant aux antibiotiques, CHU UCL Namur, 1 Avenue G. Thérasse, 5530 Yvoir, Belgium.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Evrard
authors.affiliation: Laboratoire de Bactériologie, Centre national de référence des Enterobactéries, Pseudomonas et Acinetobacter multirésistant aux antibiotiques, CHU UCL Namur, 1 Avenue G. Thérasse, 5530 Yvoir, Belgium.
authors.forename: Martin
authors.initials: M
authors.lastname: Hoebeke
authors.affiliation: Service de Bactériologie-Virologie, CNR associé de la résistance aux antibiotiques 'Entérobactéries productrices de carbapénèmases', Hôpital de Bicêtre, Faculté de Médecine Paris-Sud, Assistance Publique-Hôpitaux de Paris, Le Kremlin-Bicêtre, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Naas
authors.affiliation: Laboratoire de Bactériologie, Centre national de référence des Enterobactéries, Pseudomonas et Acinetobacter multirésistant aux antibiotiques, CHU UCL Namur, 1 Avenue G. Thérasse, 5530 Yvoir, Belgium.
authors.forename: Youri
authors.initials: Y
authors.lastname: Glupczynski
mesh_terms: D000150:Acinetobacter / Q000201:enzymology* / Q000235:genetics; D001431:Bacteriological Techniques / Q000379:methods*; D004755:Enterobacteriaceae / Q000201:enzymology* / Q000235:genetics; D046228:Microarray Analysis / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis; D011549:Pseudomonas / Q000201:enzymology* / Q000235:genetics; D012680:Sensitivity and Specificity; D013997:Time Factors; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D001618:beta-Lactamases
keywords: Carbapenemase; Cephalosporinase; Extended-spectrum β-lactamase; Microarray; β-Lactamase