title: An implantable and controlled drug-release silk fibroin nanofibrous matrix to advance the treatment of solid tumour cancers.
abstract: The development of more effective cancer therapeutic strategies are still critically required. The maximization of the therapeutic effect in combination with avoiding the severe side effects on normal tissues when using chemotherapy drugs is still an urgent problem that requires improvements urgently. Here we provide implantable and controllable drug-release that utilises silk fibroin (SF) as a nanofibrous drug delivery system (DDS) for cancer treatment. A nanofibrous structure with controllable fibre diameter (<100 nm) was produced. The drug release rate of the SF DDS was controlled by applying a post-treatment process. In vitro anti-cancer (HCT116) results indicated that curcumin (CM)-SF nanofibrous matrix had a superior anti-cancer potential when the concentration was >5 μg/mL. The mechanism could be explained by the cell cycle being held in the S phase. The toxic effect on normal cells (NCM460) was minimized by using a treatment concentration range (5-20 μg/mL). Implantation of this DDS into the tumour site inhibited the growth of solid tumour; this offers an alternative approach for novel cancer therapy.
authors.affiliation: School of Materials, The University of Manchester, Manchester M13 9PL, UK; Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Hong Kong, China.
authors.forename: Maobin
authors.initials: M
authors.lastname: Xie
authors.affiliation: Department of Colorectal Surgery, The Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510655, China.
authors.forename: Dejun
authors.initials: D
authors.lastname: Fan
authors.affiliation: Department of Colorectal Surgery, The Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510655, China.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Chen
authors.affiliation: State Key Lab of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 43000, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Department of Colorectal Surgery, The Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510655, China.
authors.forename: Xiaowen
authors.initials: X
authors.lastname: He
authors.affiliation: National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou 215123, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.affiliation: College of Chemical Engineering, Huaqiao University, Xiamen 361021, China.
authors.forename: Aizheng
authors.initials: A
authors.lastname: Chen
authors.affiliation: Department of Colorectal Surgery, The Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510655, China.
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Wu
authors.affiliation: School of Materials, The University of Manchester, Manchester M13 9PL, UK; Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Hong Kong, China.
authors.forename: Jiashen
authors.initials: J
authors.lastname: Li
authors.affiliation: Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Hong Kong, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Li
authors.affiliation: Institute of Ageing and Chronic Disease, University of Liverpool, Liverpool L7 8TX, UK.
authors.forename: John A
authors.initials: JA
authors.lastname: Hunt
authors.affiliation: School of Materials, The University of Manchester, Manchester M13 9PL, UK; Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hung Hom, Hong Kong, China. Electronic address: henry.yili@manchester.ac.uk.
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Colorectal Surgery, The Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510655, China. Electronic address: lanping@mail.sysu.edu.cn.
authors.forename: Ping
authors.initials: P
authors.lastname: Lan
mesh_terms: D065966:Absorption, Physicochemical; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D003474:Curcumin / Q000008:administration & dosage*; D003692:Delayed-Action Preparations / Q000008:administration & dosage* / Q000737:chemistry; D004058:Diffusion; D004305:Dose-Response Relationship, Drug; D005260:Female; D005349:Fibroins / Q000737:chemistry*; D045325:HCT116 Cells; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D053769:Nanocapsules / Q000008:administration & dosage / Q000737:chemistry* / Q000648:ultrastructure; D057139:Nanofibers / Q000008:administration & dosage / Q000737:chemistry* / Q000648:ultrastructure; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000473:pathology; D010316:Particle Size; D016896:Treatment Outcome
chemical_list: D000970:Antineoplastic Agents; D003692:Delayed-Action Preparations; D053769:Nanocapsules; D005349:Fibroins; D003474:Curcumin
keywords: Cancer; Curcumin; Drug delivery system; Silk