title: Anxiety-related behavior in hyperhomocysteinemia induced by methionine nutritional overload in rats: role of the brain oxidative stress.
abstract: The aim of this study was to examine the effects of a methionine-enriched diet on anxiety-related behavior in rats and to determine the role of the brain oxidative status in these alterations. Adult male Wistar rats were fed from the 30th to 60th postnatal day with standard or methionine-enriched diet (double content comparing with standard diet: 7.7 g/kg). Rats were tested in open field and light-dark tests and afterwards oxidative status in the different brain regions were determined. Hyperhomocysteinemia induced by methionine-enriched diet in this study decreased the number of rearings, as well as the time that these animals spent in the center of the open field, but increased index of thigmotaxy. Oxidative status was selectively altered in the examined regions. Lipid peroxidation was significantly increased in the cortex and nc. caudatus of rats developing hyperhomocysteinemia, but unaltered in the hippocampus and thalamus. Based on the results of this research, it could be concluded that hyperhomocysteinemia induced by methionine nutritional overload increased anxiety-related behavior in rats. These proanxiogenic effects could be, at least in part, a consequence of oxidative stress in the rat brain.
authors.affiliation: a Institute of Medical Physiology "Richard Burian", Belgrade University Faculty of Medicine, 11000 Belgrade, Serbia.
authors.forename: Dragan
authors.initials: D
authors.lastname: Hrncic
authors.affiliation: a Institute of Medical Physiology "Richard Burian", Belgrade University Faculty of Medicine, 11000 Belgrade, Serbia.
authors.forename: Jelena
authors.initials: J
authors.lastname: Mikić
authors.affiliation: a Institute of Medical Physiology "Richard Burian", Belgrade University Faculty of Medicine, 11000 Belgrade, Serbia.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Rasic-Markovic
authors.affiliation: b Institute of Clinical and Medical Biochemistry, Belgrade University Faculty of Medicine, 11000 Belgrade, Serbia.
authors.forename: Milica
authors.initials: M
authors.lastname: Velimirović
authors.affiliation: b Institute of Clinical and Medical Biochemistry, Belgrade University Faculty of Medicine, 11000 Belgrade, Serbia.
authors.forename: Tihomir
authors.initials: T
authors.lastname: Stojković
authors.affiliation: c Clinical Center of Serbia, Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia.
authors.forename: Radmila
authors.initials: R
authors.lastname: Obrenović
authors.affiliation: a Institute of Medical Physiology "Richard Burian", Belgrade University Faculty of Medicine, 11000 Belgrade, Serbia.
authors.forename: Bojana
authors.initials: B
authors.lastname: Rankov-Petrović
authors.affiliation: d Serbian Academy of Sciences and Arts, 11000 Belgrade, Serbia.
authors.forename: Veselinka
authors.initials: V
authors.lastname: Šušić
authors.affiliation: a Institute of Medical Physiology "Richard Burian", Belgrade University Faculty of Medicine, 11000 Belgrade, Serbia.
authors.forename: Dragan
authors.initials: D
authors.lastname: Djuric
authors.affiliation: b Institute of Clinical and Medical Biochemistry, Belgrade University Faculty of Medicine, 11000 Belgrade, Serbia.
authors.forename: Nataša
authors.initials: N
authors.lastname: Petronijević
authors.affiliation: a Institute of Medical Physiology "Richard Burian", Belgrade University Faculty of Medicine, 11000 Belgrade, Serbia.
authors.forename: Olivera
authors.initials: O
authors.lastname: Stanojlovic
keywords: anxiety; anxiété; behavior; comportement; hyperhomocysteinemia; hyperhomocystéinémie; methionine; méthionine; nutrition; oxidative stress; rats; stress oxydatif