title: Roles of microRNA-99 family in human glioma.
abstract: OBJECTIVE
  Deregulation of microRNA (miR)-99 family members (miR-99a, miR-99b, and miR-100) has been reported to play a crucial role in many cancer types. However, their roles in human gliomas have not been fully elucidated. This study aimed to investigate the expression patterns of miR-99a, miR-99b, and miR-100 in glioma tissues and to evaluate their expression profiles with respect to tumor progression.
  
  
  METHODS
  Quantitative real-time polymerase chain reaction was performed to detect the expression levels of miR-99a, miR-99b, and miR-100 in glioma and matched non-neoplastic brain tissues. Then, the associations of their expression with various clinicopathological features of glioma patients were statistically analyzed. Moreover, the roles of miR-99a, miR-99b, and miR-100 in regulating glioma cell migration and invasion were determined via transwell assay in vitro.
  
  
  RESULTS
  Compared with non-neoplastic brain tissues, miR-99a, miR-99b, and miR-100 expression levels were all significantly decreased in glioma tissues (all P<0.001). miR-99a-low, miR-99b-low, and miR-100-low expression more frequently occurred in glioma patients with low Karnofsky performance score (<90) and high World Health Organization grade (III-IV). Further functional experiments revealed that the enforced expression of miR-99a, miR-99b, and miR-100 resulted in the inhibition of cellular migration and invasion in glioma cells.
  
  
  CONCLUSIONS
  Our results strongly suggest that the aberrant expression of miR-99a, miR-99b, and miR-100 may be a common feature in human gliomas with aggressive clinicopathological features and may participate in malignant phenotypes of the tumors. These findings highlight the potential of the three miR-99 family members as novel therapeutic targets for human gliomas.
authors.affiliation: Department of Neurosurgery, Xiangya Hospital of Central South University, Changsha.
authors.forename: Mingyu
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, Xiangya Hospital of Central South University, Changsha.
authors.forename: Yong
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Department of Neurosurgery, Xiangya Hospital of Central South University, Changsha.
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Neurosurgery, Xiangya Hospital of Central South University, Changsha.
authors.forename: Fenghua
authors.initials: F
authors.lastname: Chen
authors.affiliation: Institute of Endocrinology and Metabolism, Second Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.
authors.forename: Zhijie
authors.initials: Z
authors.lastname: Dai
authors.affiliation: Department of Neurosurgery, Xiangya Hospital of Central South University, Changsha.
authors.forename: Shuangshi
authors.initials: S
authors.lastname: Fan
authors.affiliation: Department of Neurosurgery, Xiangya Hospital of Central South University, Changsha.
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Xiangya Hospital of Central South University, Changsha.
authors.forename: Tao
authors.initials: T
authors.lastname: Song
keywords: clinicopathological features; glioma; invasion; microRNA-99 family; migration