title: Static magnetic field enhances the viability and proliferation rate of adipose tissue-derived mesenchymal stem cells potentially through activation of the phosphoinositide 3-kinase/Akt (PI3K/Akt) pathway.
abstract: The aim of this work was to investigate the effects of 0.5T static magnetic field (sMF) on the viability and proliferation rate of human adipose-derived mesenchymal stromal stem cells (hASCs) via activation of the phosphoinositide 3-kinase/Akt (PI3K/Akt) signaling pathway. In a 7-d culture we examined cell growth kinetic and population doubling time (PDT). We also examined cell morphology and the cellular senescence markers level. Exposure to sMF enhanced the viability of these cells. However, the effect was blocked by treating the cells with LY294002, a P13K inhibitor. We compared this effect by Western Blot analysis of Akt protein expression. We also examined whether the cell response on sMF stimulation is dependent on integrin engagement and we measured integrin gene expression. Our results suggest that stimulation using sMF is a viable method to improve hASC viability. sMF is involved in mechanisms associated with controlling cell proliferative potential signaling events.
authors.affiliation: a Faculty of Veterinary Medicine , University of Environmental and Life Sciences , Wrocław , Poland.|b Faculty of Biology , University of Environmental and Life Sciences , Wrocław , Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Marędziak
authors.affiliation: c Department of Anatomy , Jagiellonian University Medical College , Krakow , Poland.|d Department of Orthopaedics and Trauma Surgery , 5th Military Clinical Hospital and Polyclinic , Krakow , Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Tomaszewski
authors.affiliation: e Faculty of Biotechnology , Laboratory of Cytobiochemistry, University of Wroclaw , Wrocław , Poland.
authors.forename: Paulina
authors.initials: P
authors.lastname: Polinceusz
authors.affiliation: f Institute of Materials Science and Applied Mechanics, Wroclaw University of Technology , Wrocław , Poland.
authors.forename: Daniel
authors.initials: D
authors.lastname: Lewandowski
authors.affiliation: b Faculty of Biology , University of Environmental and Life Sciences , Wrocław , Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Marycz
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002867:Chromones / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D039564:Integrin alphaV / Q000235:genetics; D039661:Integrin beta3 / Q000235:genetics; D060526:Magnetic Fields; D059630:Mesenchymal Stem Cells / Q000145:classification* / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009025:Morpholines / Q000494:pharmacology; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D002867:Chromones; D039564:Integrin alphaV; D039661:Integrin beta3; D009025:Morpholines; D012333:RNA, Messenger; C085911:2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt
keywords: PI3K/Akt pathway; Static magnetic field; apoptosis; integrins; mesenchymal stromal stem cells