title: The fluorescent bioprobe with aggregation-induced emission features for monitoring to carbon dioxide generation rate in single living cell and early identification of cancer cells.
abstract: A novel fluorescent probe, tris (2-(dimethylamino) ethyl)-4,4',4″-(1H-pyrrole-1,2,5-triyl) tribenzoate (TPP-TMAE), with aggregation-enhanced emission (AEE) feature showed a simple, highly selective, specific, and instant response to trace amount carbon dioxide (CO2). Because of this special characteristic, TPP-TMAE is ideal to be a biomarker for in-situ monitoring of the CO2 generation rate during the metabolism of single living cell. The rates in single living HeLa cell, MCF-7 cell, and MEF cell were 6.40 × 10(-6)±6.0 × 10(-8) μg/h, 5.78 × 10(-6)±6.0 × 10(-8) μg/h, and 4.27 × 10(-7)±4.0 × 10(-9) μg/h, respectively. The distinct responses of TPP-TMAE to CO2 generated from cancer cells and normal cells suggested TPP-TMAE as a useful tool for deeper understanding metabolism process and distinguishing cancer cells from normal cells during the early diagnosis of cancers.
authors.affiliation: School of Materials Science and Engineering, Beijing Institute of Technology, 5 South Zhongguancun Street, Beijing, 100081, China.
authors.forename: Didi
authors.initials: D
authors.lastname: Chen
authors.affiliation: School of Materials Science and Engineering, Beijing Institute of Technology, 5 South Zhongguancun Street, Beijing, 100081, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Wang
authors.affiliation: School of Materials Science and Engineering, Beijing Institute of Technology, 5 South Zhongguancun Street, Beijing, 100081, China.
authors.forename: Lichao
authors.initials: L
authors.lastname: Dong
authors.affiliation: School of Life Science, Beijing Institute of Technology, 5 South Zhongguancun Street, Beijing, 100081, China.
authors.forename: Pai
authors.initials: P
authors.lastname: Liu
authors.affiliation: School of Materials Science and Engineering, Beijing Institute of Technology, 5 South Zhongguancun Street, Beijing, 100081, China.
authors.forename: Yahui
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Materials Science and Engineering, Beijing Institute of Technology, 5 South Zhongguancun Street, Beijing, 100081, China.
authors.forename: Jianbing
authors.initials: J
authors.lastname: Shi
authors.affiliation: School of Chemistry, Beijing Institute of Technology, 5 South Zhongguancun Street, Beijing, 100081, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Feng
authors.affiliation: School of Chemistry, Beijing Institute of Technology, 5 South Zhongguancun Street, Beijing, 100081, China.
authors.forename: Junge
authors.initials: J
authors.lastname: Zhi
authors.affiliation: School of Materials Science and Engineering, Beijing Institute of Technology, 5 South Zhongguancun Street, Beijing, 100081, China. Electronic address: tongbin@bit.edu.cn.
authors.forename: Bin
authors.initials: B
authors.lastname: Tong
authors.affiliation: School of Materials Science and Engineering, Beijing Institute of Technology, 5 South Zhongguancun Street, Beijing, 100081, China. Electronic address: chdongyp@bit.edu.cn.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Dong
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D002245:Carbon Dioxide / Q000737:chemistry / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D061986:MCF-7 Cells; D008657:Metabolic Clearance Rate; D057054:Molecular Imaging / Q000379:methods*; D009374:Neoplasms, Experimental / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology*
chemical_list: D014408:Biomarkers, Tumor; D005456:Fluorescent Dyes; D002245:Carbon Dioxide
keywords: Aggregation-enhanced emission (AEE); CO(2) generation rate; Diagnosis of cancer cells; Fluorescent probe; Metabolism