title: Consequences of elevated temperature and pCO2 on insect folivory at the ecosystem level: perspectives from the fossil record.
abstract: Paleoecological studies document the net effects of atmospheric and climate change in a natural laboratory over timescales not accessible to laboratory or ecological studies. Insect feeding damage is visible on well-preserved fossil leaves, and changes in leaf damage through time can be compared to environmental changes. We measured percent leaf area damaged on four fossil leaf assemblages from the Bighorn Basin, Wyoming, that range in age from 56.1 to 52.65 million years (Ma). We also include similar published data from three US sites 49.4 to ~45 Ma in our analyses. Regional climate was subtropical or warmer throughout this period, and the second oldest assemblage (56 Ma) was deposited during the Paleocene-Eocene Thermal Maximum (PETM), a geologically abrupt global warming event caused by massive release of carbon into the atmosphere. Total and leaf-chewing damage are highest during the PETM, whether considering percent area damaged on the bulk flora, the average of individual host plants, or a single plant host that occurs at multiple sites. Another fossil assemblage in our study, the 52.65 Ma Fifteenmile Creek paleoflora, also lived during a period of globally high temperature and pCO 2, but does not have elevated herbivory. Comparison of these two sites, as well as regression analyses conducted on the entire dataset, demonstrates that, over long timescales, temperature and pCO 2 are uncorrelated with total insect consumption at the ecosystem level. Rather, the most important factor affecting herbivory is the relative abundance of plants with nitrogen-fixing symbionts. Legumes dominate the PETM site; their prevalence would have decreased nitrogen limitation across the ecosystem, buffering generalist herbivore populations against decreased leaf nutritional quality that commonly occurs at high pCO 2. We hypothesize that nitrogen concentration regulates the opposing effects of elevated temperature and CO 2 on insect abundance and thereby total insect consumption, which has important implications for agricultural practices in today's world of steadily increasing pCO 2.
authors.affiliation: Departments of Botany and Geology & Geophysics University of Wyoming Laramie Wyoming; Department of Geology and Environmental Earth Science Miami University Oxford Ohio.
authors.forename: Ellen D
authors.initials: ED
authors.lastname: Currano
authors.affiliation: Department of Geology and Environmental Earth Science Miami University Oxford Ohio.
authors.forename: Rachel
authors.initials: R
authors.lastname: Laker
authors.affiliation: Department of Geology and Environmental Earth Science Miami University Oxford Ohio; Department of Geology Baylor University Waco Texas.
authors.forename: Andrew G
authors.initials: AG
authors.lastname: Flynn
authors.affiliation: Department of Geology and Environmental Earth Science Miami University Oxford Ohio.
authors.forename: Kari K
authors.initials: KK
authors.lastname: Fogt
authors.affiliation: Department of Geology and Environmental Earth Science Miami University Oxford Ohio.
authors.forename: Hillary
authors.initials: H
authors.lastname: Stradtman
authors.affiliation: Department of Paleobiology Smithsonian Institution Washington District of Columbia.
authors.forename: Scott L
authors.initials: SL
authors.lastname: Wing
keywords: Bighorn Basin; Paleocene–Eocene Thermal Maximum; Paleogene; herbivory; legumes; plant‐insect interactions