title: Percentage of Peripheral CD19+CD24hiCD38hi Regulatory B Cells in Neonatal Sepsis Patients and Its Functional Implication.
abstract: BACKGROUND As a major cause of mortality in neonates, neonatal sepsis is often accompanied by immune dysfunctions, which are frequently caused by dysregulated T cell sub-populations. The role of regulatory B cells in neonatal sepsis, however, remains unknown. Therefore, this study investigated the percentage and functional variation of CD19+CD24hiCD38hi regulatory B cells in peripheral blood of neonatal sepsis patients in an attempt to elucidate the role of these regulatory B cells in pathogenesis of sepsis. MATERIAL AND METHODS Flow cytometry was used to quantify the percentage of CD19+CD24hiCD38hi regulatory B cells from peripheral blood samples. The correlation between B cell percentage and C reactive protein (CRP) level was analyzed. Secretion level of interleukin-10 (IL-10) and effects on the proliferation of naïve CD4+ T cells were further analyzed. RESULTS The percentage of CD19+CD24hiCD38hi regulatory B cells in neonatal sepsis patients was significantly higher compared to healthy controls (p<0.05), and was positively correlated with serum CRP level. The percentage of IL-10+ CD19+CD24hiCD38hi regulatory B cells was also higher in sepsis patients, and also had more potent inhibition on naïve CD4+ T cells (p<0.01). CONCLUSIONS The elevation of CD19+CD24hiCD38hi regulatory B cells in neonatal sepsis can inhibit body immune function and thus may participate in the pathogenesis of sepsis.
authors.affiliation: Department of Internal Medicine, Second Ward, Linyi City People's Hospital, Linyi, Shandong, China (mainland).
authors.forename: Xiao
authors.initials: X
authors.lastname: Pan
authors.affiliation: Department of Neonatal, Affiliated Hospital of Shandong Medical College, Linyi, Shandong, China (mainland).
authors.forename: Zuoquan
authors.initials: Z
authors.lastname: Ji
authors.affiliation: Department of Neonatal, Second Hospital of Shandong University, Jinan, Shandong, China (mainland).
authors.forename: Jiang
authors.initials: J
authors.lastname: Xue
mesh_terms: D051997:ADP-ribosyl Cyclase 1 / Q000097:blood*; D018941:Antigens, CD19 / Q000097:blood*; D060151:B-Lymphocytes, Regulatory / Q000276:immunology / Q000473:pathology; D002097:C-Reactive Protein / Q000378:metabolism; D051927:CD24 Antigen / Q000097:blood*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D016753:Interleukin-10 / Q000097:blood; D008297:Male; D008562:Membrane Glycoproteins / Q000097:blood*; D000071074:Neonatal Sepsis / Q000097:blood / Q000276:immunology* / Q000473:pathology; D013203:Staphylococcal Infections / Q000097:blood / Q000276:immunology / Q000473:pathology; D013921:Thrombocytopenia / Q000097:blood / Q000473:pathology
chemical_list: D018941:Antigens, CD19; C000604811:CD19 molecule, human; D051927:CD24 Antigen; C497889:CD24 protein, human; C508609:IL10 protein, human; D008562:Membrane Glycoproteins; D016753:Interleukin-10; D002097:C-Reactive Protein; C498154:CD38 protein, human; D051997:ADP-ribosyl Cyclase 1