title: S100A8 and S100A9 proteins are expressed by human corneal stromal dendritic cells.
abstract: The limbus is a remarkable anatomical site endowed with specialised functions to ensure corneal health and transparency, which is essential for exquisite vision. Cell types that contribute to homeostasis and the disease-free state of the cornea include epithelial and stromal stem cells, and antigen-presenting dendritic cells (DCs). DCs are found throughout the corneal epithelium and stroma, but the protein markers that discriminate between cells in different locations have not been properly identified. S100 proteins are expressed in normal and diseased ocular surfaces and are implicated in DC differentiation.
  
  
  
  This study used transplant quality human cadaveric donor corneas (n=6) and immunofluorescence to determine the spatial distributions of S100A8 (A8) and S100A9 (A9), and to characterise the cell types expressing these proteins.
  
  
  
  A8-expressing and A9-expressing cells were predominantly confined to the limbal stroma and represented 0.25%±0.1% and 0.39%±0.1%, respectively, of the total stromal cell population. They were phenotyped as CD45(+)/HLA-DR(+)/CD11c(+), markers characteristic of DCs. Interestingly, A8 and A9 immunoreactivity was only associated with stromal DCs, but not those entrenched in the epithelium.
  
  
  
  A8 and A9 expression may distinguish between subpopulations of DC that reside in different regions of the human cornea and may influence their maturation status.
authors.affiliation: School of Medical Sciences, University of New South Wales, Sydney, New South Wales, Australia.
authors.forename: Adam
authors.initials: A
authors.lastname: Wilkinson
authors.affiliation: School of Medical Sciences, University of New South Wales, Sydney, New South Wales, Australia.
authors.forename: Naomi
authors.initials: N
authors.lastname: Kawaguchi
authors.affiliation: School of Medical Sciences, University of New South Wales, Sydney, New South Wales, Australia.
authors.forename: Carolyn
authors.initials: C
authors.lastname: Geczy
authors.affiliation: School of Medical Sciences, University of New South Wales, Sydney, New South Wales, Australia.
authors.forename: Nick
authors.initials: N
authors.lastname: Di Girolamo
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000378:metabolism; D002102:Cadaver; D040501:Calgranulin A / Q000096:biosynthesis*; D040502:Calgranulin B / Q000096:biosynthesis*; D002452:Cell Count; D002454:Cell Differentiation; D003319:Corneal Stroma / Q000166:cytology / Q000378:metabolism*; D003713:Dendritic Cells / Q000166:cytology / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged
chemical_list: D015415:Biomarkers; D040501:Calgranulin A; D040502:Calgranulin B
keywords: Cornea