title: Purification and Some Properties of an α-Amylase from an Anaerobic Bacterium Isolated from Coastal Sediment.
abstract: A marine, obligate anaerobic bacterium, SS71, isolated from a coastal sediment, was a Gram-positive, asporogenous, rod-shaped organism with a G + C mol% of 37.3 ±0.1. This bacterium produced an α-amylase with a molecular mass of 91 kDa and an isoelectric point of 4.3. The α-amylase had an optimal pH of 7.0 and an optimal temperature of 35°C. The enzyme activity was promoted by 0.5-2.0% NaCl.
authors.affiliation: a Department of Marine Science and Resources , Nikon University , Kameino, Fujisawa, Kanagawa 252 , Japan.
authors.forename: H
authors.initials: H
authors.lastname: Sugita
authors.affiliation: a Department of Marine Science and Resources , Nikon University , Kameino, Fujisawa, Kanagawa 252 , Japan.
authors.forename: A
authors.initials: A
authors.lastname: Kuruma
authors.affiliation: a Department of Marine Science and Resources , Nikon University , Kameino, Fujisawa, Kanagawa 252 , Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Deguchi
keywords: coastal sediment; marine bacteria; obligate anaerobe; α-amylase