title: Pathogenesis of Renal Lesions in Chickens After Experimental Infection With 9a5b Newcastle Disease Virus Mutant Isolate.
abstract: In this study, we investigated the pathogenesis of Newcastle disease virus (NDV) in the chicken kidney. Twenty-six 32-day-old specific pathogen-free chickens were intranasally inoculated with the 9a5b NDV mutant isolate. Kidney tissue samples, collected at 6 and 12 hours postinoculation (hpi) and 1, 2, 3, 5, and 10 days postinoculation (dpi), were analyzed by histopathology, immunohistochemistry (IHC), reverse transcription polymerase chain reaction (RT-PCR), and virus titration. Histopathologically, tubulointerstitial nephritis was detected in the renal cortex and predominantly in the medulla. Nephrotropism of 9a5b NDV was confirmed by IHC, RT-PCR, and virus isolation. Massive degenerative changes and infiltration of CD3-immunopositive cells accompanied replication of the 9a5b NDV isolate in chicken kidneys. In conclusion, pathological changes that were caused by NDV in chicken kidneys were similar to those caused by avian influenza virus, infectious bronchitis virus, and avian nephritis virus, and this highlights the importance of including NDV in the differential diagnosis of kidney disease in chickens.
authors.affiliation: 1 Department of Veterinary Pathology, Faculty of Agriculture, Tottori University, Minami, Koyama-cho, Tottori, Japan.|2 The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan.|3 Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Sadat City, Menoufiya, Egypt.
authors.forename: A
authors.initials: A
authors.lastname: El-Bahrawy
authors.affiliation: 1 Department of Veterinary Pathology, Faculty of Agriculture, Tottori University, Minami, Koyama-cho, Tottori, Japan.|3 Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Sadat City, Menoufiya, Egypt.
authors.forename: A
authors.initials: A
authors.lastname: Zaid
authors.affiliation: 1 Department of Veterinary Pathology, Faculty of Agriculture, Tottori University, Minami, Koyama-cho, Tottori, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Sunden
authors.affiliation: 4 Department of Veterinary Pathology, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Sakurai
authors.affiliation: 5 Department of Veterinary Public Health, Faculty of Agriculture, Tottori University, Minami, Koyama-cho, Tottori, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ito
authors.affiliation: 5 Department of Veterinary Public Health, Faculty of Agriculture, Tottori University, Minami, Koyama-cho, Tottori, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ito
authors.affiliation: 1 Department of Veterinary Pathology, Faculty of Agriculture, Tottori University, Minami, Koyama-cho, Tottori, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Morita
mesh_terms: D000818:Animals; D002645:Chickens / Q000821:virology; D007668:Kidney / Q000473:pathology* / Q000821:virology; D007672:Kidney Cortex / Q000473:pathology / Q000821:virology; D008297:Male; D009154:Mutation / Q000235:genetics; D009395:Nephritis, Interstitial / Q000473:pathology / Q000662:veterinary / Q000821:virology; D009521:Newcastle Disease / Q000473:pathology* / Q000821:virology; D009522:Newcastle disease virus / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
keywords: Newcastle disease; chicken; kidney; nephrotropism