title: Osteoprotegerin Promotes Cementoblastic Activity of Murine Cementoblast Cell Line in vitro.
abstract: OBJECTIVE
  To investigate the effect of osteoprotegerin (OPG) on the cementoblastic activity of a clonal population of immortalised murine cementoblasts (OCCM-30) in vitro.
  
  
  METHODS
  OCCM-30 cells were transiently transfected with the mouse OPG using the Avalanche transfection reagent. The ectopic expression of OPG was confirmed by enzyme-linked immunosorbent assay and quantitative polymerase chain reaction. The cell counting Kit-8 assay was used to investigate the effect of OPG on cell proliferation. The expression levels of cementoblastic-related mRNA and protein in the transfected OCCM-30 cells were detected using real-time PCR, Western blotting and immunohistochemical staining.
  
  
  RESULTS
  Satisfactory transfection efficiency was observed 48 h after transfection. The results of the cell proliferation assay indicated that the expansion rate of the OPG transfection group was greater than that of the control group at both 72 h and 96 h. The mRNA levels of osterix (Osx), protein kinase B (Akt1), cementum attachment protein (CAP) and osteopontin (Opn) were significantly upregulated (P < 0.05) in the OPG group. Protein levels of OPN, bone sialoprotein II (BSP II), osteocalcin (OC) and CAP, which are responsible for osteogenetic and cementoblastic activity, were significantly increased in the OPG-overexpressing group.
  
  
  CONCLUSIONS
  Overexpression of OPG in OCCM-30 cells promotes cementoblastic activity.
authors.forename: Ying Ying
authors.initials: YY
authors.lastname: Zhang
authors.forename: Hua Xiang
authors.initials: HX
authors.lastname: Zhao
authors.forename: Zhi Bin
authors.initials: ZB
authors.lastname: Chen
authors.forename: Jiu Xiang
authors.initials: JX
authors.lastname: Lin
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D015519:Bone Density; D002460:Cell Line; D049109:Cell Proliferation; D003739:Dental Cementum / Q000187:drug effects* / Q000378:metabolism*; D051379:Mice; D053244:Osteoprotegerin / Q000235:genetics / Q000378:metabolism*
chemical_list: D053244:Osteoprotegerin