title: SNP haplotyping technique for evaluation of MGP 5' UTR power in osteoblast cells.
abstract: Matrix Gla protein (MGP) is involved in calcium trafficking and arterial calcification. The aim of study was to investigate the role of three polymorphisms within the MGP gene promoter region on reporter gene (luciferase) expression level. The fragments containing rs1800799 (C/T), rs1800802 (T/C), and rs1800801 (G/A) sites were constructed and transferred into human G292 osteoblast cells using pGL3-Basic plasmid. The reporter gene expression was calculated for the high and low frequency polymorphic haplotypes (CTG and TCA, respectively). Results showed that the reporter gene expression levels are not statistically different (p > 0.3). We concluded that the investigated polymorphic sites are not able to change the gene expression pattern in human G292 osteoblast cells.
authors.affiliation: Research Center for Non-Communicable Diseases and Biochemistry Department, Jahrom University of Medical Sciences, Jahrom, Iran.
authors.forename: Abazar
authors.initials: A
authors.lastname: Roustazadeh
authors.affiliation: Virology Department, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Seyed Hamidreza
authors.initials: SH
authors.lastname: Monavari
authors.affiliation: Biochemistry Department, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Seyed Reza
authors.initials: SR
authors.lastname: Hosseini Fard
authors.affiliation: Biochemistry Department, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Parisa
authors.initials: P
authors.lastname: Hassanpour
authors.affiliation: Biochemistry Department, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Amaneh
authors.initials: A
authors.lastname: Yarnazari
authors.affiliation: Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran ; Biochemistry Department, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Najafi
keywords: Haplotype; Matrix Gla protein (MGP); Osteoblast; Promoter; Single nucleotide polymorphism (SNP)