title: Two Inducible Prophages of an Antarctic Pseudomonas sp. ANT_H14 Use the Same Capsid for Packaging Their Genomes - Characterization of a Novel Phage Helper-Satellite System.
abstract: Two novel prophages ФAH14a and ФAH14b of a psychrotolerant Antarctic bacterium Pseudomonas sp. ANT_H14 have been characterized. They were simultaneously induced with mitomycin C and packed into capsids of the same size and protein composition. The genome sequences of ФAH14a and ФAH14b have been determined. ФAH14b, the phage with a smaller genome (16,812 bp) seems to parasitize ФAH14a (55,060 bp) and utilizes its capsids, as only the latter encodes a complete set of structural proteins. Both viruses probably constitute a phage helper-satellite system, analogous to the P2-P4 duo. This study describes the architecture and function of the ФAH14a and ФAH14b genomes. Moreover, a functional analysis of a ФAH14a-encoded lytic enzyme and a DNA methyltransferase was performed. In silico analysis revealed the presence of the homologs of ФAH14a and ФAH14b in other Pseudomonas genomes, which may suggest that helper-satellite systems related to the one described in this work are common in pseudomonads.
authors.affiliation: Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw, Warsaw, Poland.
authors.forename: Lukasz
authors.initials: L
authors.lastname: Dziewit
authors.affiliation: Department of Virology, Institute of Microbiology, Faculty of Biology, University of Warsaw, Warsaw, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Radlinska
mesh_terms: D000864:Antarctic Regions; D001435:Bacteriophages / Q000235:genetics*; D002213:Capsid / Q000378:metabolism; D036022:Capsid Proteins / Q000235:genetics; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics; D004252:DNA Mutational Analysis; D004279:DNA, Viral / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D006378:Helper Viruses / Q000235:genetics; D039002:Prophages / Q000235:genetics*; D011549:Pseudomonas / Q000821:virology*; D012526:Satellite Viruses / Q000235:genetics*; D012988:Soil Microbiology; D014775:Virus Activation
chemical_list: D036022:Capsid Proteins; D004279:DNA, Viral; D004248:DNA (Cytosine-5-)-Methyltransferases