title: Expression of podoplanin and TGF-beta in glandular odontogenic cyst and its comparison with developmental and inflammatory odontogenic cystic lesions.
abstract: BACKGROUND
  The number of studies investigating the immunohistochemical characteristics of glandular odontogenic cysts (GOCs) is limited, due to its rarity. TGF-beta has been suggested to induce podoplanin expression in some lesions. We aimed to evaluate and compare podoplanin and TGF-beta expression in GOC and other odontogenic cystic lesions.
  
  
  METHODS
  A total of 43 samples including five GOCs, 10 dentigerous cysts (DCs), eight unicystic ameloblastoma (UAs), and 20 radicular cysts (RCs) were selected and subjected to immunohistochemical staining using monoclonal antibodies against podoplanin and TGF-beta. Kruskal-Wallis test and Mann-Whitney U-test were used for statistical analysis along with Bonferroni for adjusting P-values (P < 0.05).
  
  
  RESULTS
  Podoplanin immunoreactivity was observed in 80%, 70%, and 100% of DCs, RCs, and UAs, respectively, while none of the GOCs were positive for this marker (P = 0.004). Significant differences were only found in the GOC specimens. TGF-beta positivity occurred in the capsule and epithelium of all GOCs and DCs, while RCs and UAs demonstrated different expression percentages in the capsular and epithelial tissues. Epithelial TGF-beta showed significant differences among the studied lesions (P = 0.007) with the main difference found between DCs with RCs and DCs with UAs.
  
  
  CONCLUSIONS
  Lack of podoplanin expression might be involved in the characteristic histologic and behavioral features of GOC, which seems to be unrelated to TGF-beta expression.
authors.affiliation: Dental Research Center, Dentistry Research Institute, Tehran University of Medical Science, Tehran, Iran.
authors.forename: Mojgan
authors.initials: M
authors.lastname: Alaeddini
authors.affiliation: Department of Oral and Maxillofacial Pathology, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Nosratollah
authors.initials: N
authors.lastname: Eshghyar
authors.affiliation: Dental Research Center, Dentistry Research Institute, Tehran University of Medical Science, Tehran, Iran.
authors.forename: Shahroo
authors.initials: S
authors.lastname: Etemad-Moghadam
mesh_terms: D000564:Ameloblastoma / Q000378:metabolism / Q000473:pathology; D003803:Dentigerous Cyst / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007571:Jaw Diseases / Q000378:metabolism*; D007573:Jaw Neoplasms / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism*; D009807:Odontogenic Cysts / Q000378:metabolism* / Q000473:pathology; D011842:Radicular Cyst / Q000378:metabolism / Q000473:pathology; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D000098853:Podoplanin
chemical_list: D008562:Membrane Glycoproteins; D016212:Transforming Growth Factor beta; C117468:PDPN protein, human; D000098853:Podoplanin
keywords: TGF-beta; glandular odontogenic cyst; odontogenic cysts; podoplanin