title: Downregulated PIRH2 Can Decrease the Proliferation of Breast Cancer Cells.
abstract: We undertook this study to investigate the influence of PIRH2 (p53-induced RING-H2) protein on the proliferation and cell cycle of breast cancer cell lines.
  
  
  
  PIRH2 expression was detected by Western blot analysis, immunohistochemistry (IHC) and Kaplan-Meier curve analysis. Cell proliferation was assessed by cell counting kit-8 (CCK-8). Cell cycle control was analyzed by flow cytometry.
  
  
  
  PIRH2 was up-regulated in breast cancer tissues and cell lines and up-regulated PIRH2 was highly associated with tumor size, grade, ER, and Ki-67. Moreover, Kaplan-Meier curve showed that up-regulated PIRH2 was related to the poor overall survival of patients with breast carcinoma. When the expression of PIRH2 was inhibited by siRNA transfection, cell proliferation was reduced. In addition, the number of G0/G1 phase cells was increased, but G2/M cells were not affected significantly.
  
  
  
  Decrease of PIRH2 expression in the breast cancer cell line MDA-MB-231 resulted in reduced tumor cell growth via the inhibition of cell proliferation and the interruption of cell cycle transition.
authors.affiliation: Department of Pathology, Nantong University Tumor Hospital, Nantong, Jiangsu Province, People's Republic of China.
authors.forename: Shuyun
authors.initials: S
authors.lastname: Yang
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong, Jiangsu Province, People's Republic of China; Department of Oncology, Affiliated Hospital of Nantong University, Nantong, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of General Surgery, The Second People's Hospital of Nantong, Nantong, China.
authors.forename: Fulan
authors.initials: F
authors.lastname: Sun
authors.affiliation: Department of General Surgery, The Affiliated Hospital of Nantong University, Nantong, Jiangsu, People's Republic of China.
authors.forename: Qichao
authors.initials: Q
authors.lastname: Ni
authors.affiliation: Department of General Surgery, The Affiliated Hospital of Nantong University, Nantong, Jiangsu, People's Republic of China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Oncology, Affiliated Hospital of Nantong University, Nantong, China.
authors.forename: Yeqing
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of General Surgery, The Affiliated Hospital of Nantong University, Nantong, Jiangsu, People's Republic of China.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Cardiothoracic Surgery, Affiliated Hospital of Nantong University, Nantong, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Liu
authors.affiliation: Department of Cardiothoracic Surgery, Affiliated Hospital of Nantong University, Nantong, China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of General Surgery, The Affiliated Hospital of Nantong University, Nantong, Jiangsu, People's Republic of China.
authors.forename: Jian
authors.initials: J
authors.lastname: Qiu
authors.affiliation: Department of Oncology, Affiliated Hospital of Nantong University, Nantong, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of Oncology, Affiliated Hospital of Nantong University, Nantong, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Hua
authors.affiliation: Department of General Surgery, The Affiliated Hospital of Nantong University, Nantong, Jiangsu, People's Republic of China. Electronic address: jsnthezhixian@sina.com.
authors.forename: Zhixian
authors.initials: Z
authors.lastname: He
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D005260:Female; D059585:G1 Phase Cell Cycle Checkpoints; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D034741:RNA, Small Interfering / Q000235:genetics; D014162:Transfection; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D034741:RNA, Small Interfering; C485369:RCHY1 protein, human; D044767:Ubiquitin-Protein Ligases
keywords: Breast neoplasms; Cell cycle; Proliferation; Ubiquitin; p53-induced RING-H2 (PIRH2)