title: The draft genome of MD-2 pineapple using hybrid error correction of long reads.
abstract: The introduction of the elite pineapple variety, MD-2, has caused a significant market shift in the pineapple industry. Better productivity, overall increased in fruit quality and taste, resilience to chilled storage and resistance to internal browning are among the key advantages of the MD-2 as compared with its previous predecessor, the Smooth Cayenne. Here, we present the genome sequence of the MD-2 pineapple (Ananas comosus (L.) Merr.) by using the hybrid sequencing technology from two highly reputable platforms, i.e. the PacBio long sequencing reads and the accurate Illumina short reads. Our draft genome achieved 99.6% genome coverage with 27,017 predicted protein-coding genes while 45.21% of the genome was identified as repetitive elements. Furthermore, differential expression of ripening RNASeq library of pineapple fruits revealed ethylene-related transcripts, believed to be involved in regulating the process of non-climacteric pineapple fruit ripening. The MD-2 pineapple draft genome serves as an example of how a complex heterozygous genome is amenable to whole genome sequencing by using a hybrid technology that is both economical and accurate. The genome will make genomic applications more feasible as a medium to understand complex biological processes specific to pineapple.
authors.affiliation: Biotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, Malaysia.
authors.forename: Raimi M
authors.initials: RM
authors.lastname: Redwan
authors.affiliation: Novocraft Technology Sdn. Bhd., C-23A-05, Jalan 19/1, Seksyen 19, 46300 Petaling Jaya, Selangor, Malaysia.
authors.forename: Akzam
authors.initials: A
authors.lastname: Saidin
authors.affiliation: Biotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, Malaysia.
authors.forename: S Vijay
authors.initials: SV
authors.lastname: Kumar
keywords: fruit ripening; hybrid assembly; pineapple; plant genome