title: Increased Cellulose Production from Sucrose by Acetobacter after Introducing the Sucrose Phosphorylase Gene.
abstract: A sucrose phosphorylase (SPase) gene derived from Leuconostoc mesenteroides was introduced into a cellulose-producing Acetobacter strain and expressed under the lac promoter. The activity of the SPase was detected in extracts of the transformed cells and cellulose production from sucrose by the cells was found to have increased, which strongly suggests that the increase was the result of the new metabolizing pathway. Furthermore, the level of SPase expression was increased by altering the length of the lac promoter.
authors.affiliation: a Bio-Polymer Research Co., Ltd.
authors.forename: N
authors.initials: N
authors.lastname: Tonouchi
authors.affiliation: b Department of Biotechnology, The University of Tokyo.
authors.forename: S
authors.initials: S
authors.lastname: Horinouchi
authors.affiliation: a Bio-Polymer Research Co., Ltd.
authors.forename: T
authors.initials: T
authors.lastname: Tsuchida
authors.affiliation: a Bio-Polymer Research Co., Ltd.
authors.forename: F
authors.initials: F
authors.lastname: Yoshinaga
keywords: Acetobacter; cellulose; gene introduction; sucrose phosphorylase