title: Histamine-producing Lactobacillus parabuchneri strains isolated from grated cheese can form biofilms on stainless steel.
abstract: The consumption of food containing large amounts of histamine can lead to histamine poisoning. Cheese is one of the most frequently involved foods. Histamine, one of the biogenic amines (BAs) exhibiting the highest safety risk, accumulates in food contaminated by microorganisms with histidine decarboxylase activity. The origin of these microorganisms may be very diverse with contamination likely occurring during post-ripening processing, but the microorganisms involved during this manufacturing step have never been identified. The present work reports the isolation of 21 histamine-producing Lactobacillus parabuchneri strains from a histamine-containing grated cheese. PCR revealed that every isolate carried the histidine decarboxylase gene (hdcA). Eight lineages were identified based on the results of genome PFGE restriction analysis plus endonuclease restriction profile analysis of the carried plasmids. Members of all lineages were able to form biofilms on polystyrene and stainless steel surfaces. L. parabuchneri is therefore an undesirable species in the dairy industry; the biofilms it can produce on food processing equipment represent a reservoir of histamine-producing bacteria and thus a source of contamination of post-ripening-processed cheeses.
authors.affiliation: Instituto de Productos Lácteos de Asturias, IPLA-CSIC, Paseo Rio Linares s/n, 33300 Villaviciosa, Spain.
authors.forename: Maria
authors.initials: M
authors.lastname: Diaz
authors.affiliation: Instituto de Productos Lácteos de Asturias, IPLA-CSIC, Paseo Rio Linares s/n, 33300 Villaviciosa, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Del Rio
authors.affiliation: Instituto de Productos Lácteos de Asturias, IPLA-CSIC, Paseo Rio Linares s/n, 33300 Villaviciosa, Spain.
authors.forename: Esther
authors.initials: E
authors.lastname: Sanchez-Llana
authors.affiliation: Instituto de Productos Lácteos de Asturias, IPLA-CSIC, Paseo Rio Linares s/n, 33300 Villaviciosa, Spain.
authors.forename: Victor
authors.initials: V
authors.lastname: Ladero
authors.affiliation: Instituto de Productos Lácteos de Asturias, IPLA-CSIC, Paseo Rio Linares s/n, 33300 Villaviciosa, Spain.
authors.forename: Begoña
authors.initials: B
authors.lastname: Redruello
authors.affiliation: Instituto de Productos Lácteos de Asturias, IPLA-CSIC, Paseo Rio Linares s/n, 33300 Villaviciosa, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Fernández
authors.affiliation: Instituto de Productos Lácteos de Asturias, IPLA-CSIC, Paseo Rio Linares s/n, 33300 Villaviciosa, Spain. Electronic address: mcm@ipla.csic.es.
authors.forename: M Cruz
authors.initials: MC
authors.lastname: Martin
authors.affiliation: Instituto de Productos Lácteos de Asturias, IPLA-CSIC, Paseo Rio Linares s/n, 33300 Villaviciosa, Spain.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Alvarez
mesh_terms: D018441:Biofilms / Q000254:growth & development*; D002611:Cheese / Q000032:analysis / Q000382:microbiology*; D016521:Electrophoresis, Gel, Pulsed-Field; D005506:Food Contamination / Q000517:prevention & control; D005511:Food Handling; D005516:Food Microbiology; D006632:Histamine / Q000096:biosynthesis*; D006640:Histidine Decarboxylase / Q000235:genetics; D007778:Lactobacillus / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism / Q000502:physiology*; D016133:Polymerase Chain Reaction; D011137:Polystyrenes; D013193:Stainless Steel
chemical_list: D011137:Polystyrenes; D013193:Stainless Steel; D006632:Histamine; D006640:Histidine Decarboxylase
keywords: Biofilms; Biogenic amines; Cheese; Histamine; Lactobacillus buchneri; Lactobacillus parabuchneri