title: EPCs enhance angiogenesis in renal regeneration.
abstract: Decellularized renal scaffolds have previously been used for renal regeneration following partial nephrectomy, in which angiogenesis played a key role. In this study, rats underwent partial nephrectomy and repaired with decellularized renal scaffolds. Subsequently, the labeled EPCs were intravenously injected into rats in EPCs group, and the control group received an equal amount of phosphate-buffer saline (PBS). We chose 1, 2 and 4 weeks post operation as time point. Average microvascular density (aMVD) analyses revealed higher angiogenesis in EPCs group compared with the control group. The expression of angiogenic growth factors including vascular endothelial growth factor (VEGF), platelet derived growth factor (PDGF) and hypoxia-inducible factors 1-alpha (HIF-1α), was generally higher in the EPCs group in all weeks (1, 2 and 4), and peaked in week 2. EPCs were observed to home into renal injury site, promoting angiogenesis across the renal parenchyma-scaffold interface to be potentially used as bridges for EPCs to migrate into the implanted scaffolds. Administration of exogenous EPCs promotes angiogenesis and vasculogenesis in decellularized renal scaffolds-mediated renal regeneration, providing adequate microenvironment for kidney recovery post renal injury.
authors.affiliation: Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou, China.|Medical School of Ningbo University, Ningbo, China.|Department of Hand Surgery, Ningbo No.6 Hospital, Ningbo, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou, China.|Anatomy Department, Wenzhou Medical University, Wenzhou, China.
authors.forename: Yaling
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou, China.|Medical School of Ningbo University, Ningbo, China.|Department of Hand Surgery, Ningbo No.6 Hospital, Ningbo, China.
authors.forename: Miaozhong
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Anatomy, King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia.|Department of Medical Neuroscience, Dalhousie University, Nova Scotia, Canada.
authors.forename: Ali
authors.initials: A
authors.lastname: Alkhawaji
authors.affiliation: Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou, China.|Medical School of Ningbo University, Ningbo, China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Chen
authors.affiliation: Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou, China.|Anatomy Department, Wenzhou Medical University, Wenzhou, China.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Liu
authors.affiliation: Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou, China.|Anatomy Department, Wenzhou Medical University, Wenzhou, China.
authors.forename: Junqun
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou, China.|Anatomy Department, Wenzhou Medical University, Wenzhou, China.
authors.forename: Jianse
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou, China.|Anatomy Department, Wenzhou Medical University, Wenzhou, China.
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou, China.|Anatomy Department, Wenzhou Medical University, Wenzhou, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Li
authors.affiliation: Medical School of Ningbo University, Ningbo, China.|Department of Hand Surgery, Ningbo No.6 Hospital, Ningbo, China.
authors.forename: Weiwen
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou, China.|Anatomy Department, Wenzhou Medical University, Wenzhou, China.|Institute of Neuroscience, Wenzhou Medical University, Wenzhou, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Mei
mesh_terms: D000818:Animals; D002465:Cell Movement; D066026:Endothelial Progenitor Cells; D007668:Kidney / Q000601:surgery; D008297:Male; D018919:Neovascularization, Physiologic / Q000502:physiology*; D009392:Nephrectomy; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration; D033581:Stem Cell Transplantation / Q000379:methods; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds
keywords: Pathology Section; angiogenesis; decellularized scaffolds; endothelial progenitor cells; homing