title: Synthesis and Evaluation of Biotinylated Bivalent HistoBlood Group Antigens for Capturing Human Noroviruses.
abstract: A panel of biotinylated bivalent H-type glycans that have been reported as binding ligands for human noroviruses were synthesized using a modular synthetic strategy. These glycoconjugates were attached to streptavidin-coated magnetic beads and used to recover human norovirus from fecal samples using a magnetic bead-based assay. The biotinylated bivalent glycans synthesized for this study exhibited similar or better capturing ability when compared to commercial biotinylated glycopolymers.
authors.affiliation: 788 Petit Science Center, Department of Chemistry, Georgia State University , Atlanta, Georgia 30302-4098, United States.
authors.forename: Abasaheb N
authors.initials: AN
authors.lastname: Dhawane
authors.affiliation: Division of Viral Diseases, Centers for Disease Control and Prevention , 1600 Clifton Road, Atlanta, Georgia 30329, United States.
authors.forename: Marta
authors.initials: M
authors.lastname: Diez-Valcarce
authors.affiliation: 788 Petit Science Center, Department of Chemistry, Georgia State University , Atlanta, Georgia 30302-4098, United States.
authors.forename: Bharat P
authors.initials: BP
authors.lastname: Gurale
authors.affiliation: 788 Petit Science Center, Department of Chemistry, Georgia State University , Atlanta, Georgia 30302-4098, United States.
authors.forename: Hieu
authors.initials: H
authors.lastname: Dinh
authors.affiliation: Division of Viral Diseases, Centers for Disease Control and Prevention , 1600 Clifton Road, Atlanta, Georgia 30329, United States.
authors.forename: Jan
authors.initials: J
authors.lastname: Vinjé
authors.affiliation: 788 Petit Science Center, Department of Chemistry, Georgia State University , Atlanta, Georgia 30302-4098, United States.
authors.forename: Suri S
authors.initials: SS
authors.lastname: Iyer
mesh_terms: D019921:Biotinylation; D001789:Blood Group Antigens / Q000737:chemistry* / Q000378:metabolism*; D060326:Chemistry Techniques, Synthetic; D005243:Feces / Q000821:virology; D006001:Glycoconjugates / Q000378:metabolism; D006801:Humans; D008958:Models, Molecular; D029322:Norovirus / Q000302:isolation & purification* / Q000378:metabolism*; D011487:Protein Conformation
chemical_list: D001789:Blood Group Antigens; D006001:Glycoconjugates