title: PGMA-based gene carriers with lipid molecules.
abstract: Lipids, as the greatest constituent in cell membranes, have been widely used for biomedical applications because of their excellent biological properties. The introduction of membrane lipid molecules into gene vectors would embody greater biocompatibility, cellular uptake and transfection efficiency. In this work, one flexible strategy for readily conjugating lipid molecules with polycations was proposed based on atom transfer radical polymerization to produce a series of cholesterol (CHO)- and phosphatidylinositol (PI)-terminated ethanolamine-functionalized poly(glycidyl methacrylate)s, namely CHO-PGEAs and PI-PGEAs, as effective gene carriers. CHO-PGEAs and PI-PGEAs truly demonstrated much better transfection performances compared to linear ethanolamine-functionalized poly(glycidyl methacrylate) (denoted as BUCT-PGEA) counterparts and traditional standard branched polythylenimine (PEI, 25 kDa). In addition, the good antitumor effects of CHO-PGEA and PI-PGEA were confirmed with suppressor tumor gene p53 systems in vitro and in vivo. The present work could provide a new strategy to develop effective cationic conjugation of lipid molecules for gene therapy.
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology (BUCT), Beijing 100029, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Xu
authors.forename: Bingran
authors.initials: B
authors.lastname: Yu
authors.forename: Hao
authors.initials: H
authors.lastname: Hu
authors.forename: Muhammad Naeem
authors.initials: MN
authors.lastname: Nizam
authors.forename: Wei
authors.initials: W
authors.lastname: Yuan
authors.forename: Jie
authors.initials: J
authors.lastname: Ma
authors.forename: Fu-Jian
authors.initials: FJ
authors.lastname: Xu
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry; D019556:COS Cells; D002470:Cell Survival; D002784:Cholesterol / Q000737:chemistry; D005260:Female; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D050997:Nanomedicine; D010716:Phosphatidylinositols / Q000737:chemistry; D011073:Polyamines / Q000737:chemistry; D000071228:Polyelectrolytes; D011109:Polymethacrylic Acids / Q000737:chemistry; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D002784:Cholesterol; D008055:Lipids; D010716:Phosphatidylinositols; D011073:Polyamines; D000071228:Polyelectrolytes; D011109:Polymethacrylic Acids; D016159:Tumor Suppressor Protein p53; C042535:polyglycidyl methacrylate