title: Identification of Candida species and susceptibility testing with Sensititre YeastOne microdilution panel to 9 antifungal agents.
abstract: OBJECTIVE
  To determine the species incidence and susceptibility pattern to 9 antifungal agents of yeasts isolated from various clinical specimens of colonized or infected patients treated in the coronary and surgical intensive care units (ICU).
  
  
  METHODS
  A total of 421 ICU patients were treated at the Cardiology Institute, Istanbul University, Istanbul, Turkey between June 2013 and May 2014, and 44 Candida species were isolated from blood, urine, endotracheal aspiration fluid, sputum, and wounds of 16 ICU patients. Identification of Candida was performed using CHROMagar. Antifungal susceptibility was determined by a Sensititre YeastOne colorimetric microdilution panel.
  
  
  RESULTS
  Candida albicans (C. albicans) was the most commonly observed microorganism 23 (54%); the other microorganisms isolated were Candida tropicalis 12 (27%), Candida glabrata 5 (11%), Candida parapsilosis 1 (2%), Candida lusitaniae 1 (2%), Candida sake 1 (2%), and Geotrichum capitatum 1 (2%). All isolates were susceptible to amphotericin B and 5-flucytosine. Geotrichum capitatum excepted, the other isolates were also susceptible to anidulafungin, micafungin, and caspofungin. Candida parapsilosis was found to be susceptible to all the studied antifungals. High MIC rates for azole group of antifungal drugs were found for C. albicans, C. tropicalis, and C. glabrata. The rate of colonisation was 3.8% (16/421). Only 0.7% (3/421) patients out of a total of 421 developed candidemia.
  
  
  CONCLUSIONS
  We found that the yeast colonization and infection rates of patients in our ICUs are very low. Candida albicans is still the most common species. We detected a decreasing susceptibility to azole compounds.
authors.affiliation: Department of Cardiovascular Surgery, Istanbul University Cardiology Institute, Istanbul, Turkey. E-mail. eates2002@yahoo.com.
authors.forename: Emine
authors.initials: E
authors.lastname: Kucukates
authors.forename: Nuh N
authors.initials: NN
authors.lastname: Gultekin
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Alisan
authors.forename: Nur
authors.initials: N
authors.lastname: Hondur
authors.forename: Recep
authors.initials: R
authors.lastname: Ozturk
mesh_terms: D000368:Aged; D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests
chemical_list: D000935:Antifungal Agents