title: A catalogue of 136 microbial draft genomes from Red Sea metagenomes.
abstract: Earth is expected to continue warming and the Red Sea is a model environment for understanding the effects of global warming on ocean microbiomes due to its unusually high temperature, salinity and solar irradiance. However, most microbial diversity analyses of the Red Sea have been limited to cultured representatives and single marker gene analyses, hence neglecting the substantial uncultured majority. Here, we report 136 microbial genomes (completion minus contamination is ≥50%) assembled from 45 metagenomes from eight stations spanning the Red Sea and taken from multiple depths between 10 to 500 m. Phylogenomic analysis showed that most of the retrieved genomes belong to seven different phyla of known marine microbes, but more than half representing currently uncultured species. The open-access data presented here is the largest number of Red Sea representative microbial genomes reported in a single study and will help facilitate future studies in understanding the physiology of these microorganisms and how they have adapted to the relatively harsh conditions of the Red Sea.
authors.affiliation: Red Sea Research Center, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.
authors.forename: Mohamed F
authors.initials: MF
authors.lastname: Haroon
authors.affiliation: Red Sea Research Center, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.|Department of Pediatrics, University of California, San Diego, California 92037, USA.
authors.forename: Luke R
authors.initials: LR
authors.lastname: Thompson
authors.affiliation: Australian Centre for Ecogenomics, School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Queensland 4072, Australia.
authors.forename: Donovan H
authors.initials: DH
authors.lastname: Parks
authors.affiliation: Australian Centre for Ecogenomics, School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Queensland 4072, Australia.|Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland 4072, Australia.
authors.forename: Philip
authors.initials: P
authors.lastname: Hugenholtz
authors.affiliation: Red Sea Research Center, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Saudi Arabia.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Stingl
mesh_terms: D001419:Bacteria / Q000235:genetics; D017753:Ecosystem; D064349:Genome, Microbial; D019156:Indian Ocean; D054892:Metagenome; D056186:Metagenomics; D064307:Microbiota; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D012623:Seawater; D017422:Sequence Analysis, DNA; D013696:Temperature
chemical_list: D012336:RNA, Ribosomal, 16S