title: [Microdevice for the investigation of high-glucose induced lifespan and the protective effect of polydatin in C. elegans].
abstract: Caenorhabditis elegans (C. elegans) has been widely used as a model organism for biomedical research due to its sufficient homology with human at molecular or genomic level. In this work, we describe a microfluidic device not only to investigate the response of C. elegans including lifespan and oxidative stress, but also to evaluate the protective effect of polydatin induced by high-glucose condition. It was found that the mean lifespan of worms was significantly reduced and the oxidative stress protein GST-4 was increased in worms that are subjected to high glucose. However, a certain dose of polydatin could weaken the increased oxidative stress induced by high-glucose and extend the lifespan, indicating the protective effect of polydatin against the toxic of high-glucose. The established approach is simple to operate, easy for realtime imaging and multiparatemer evaluations in parallel, providing a potential platform for drug evaluation/screening in a high throughput format at single animal resolution.
authors.forename: Guoli
authors.initials: G
authors.lastname: Zhu
authors.forename: Fangchao
authors.initials: F
authors.lastname: Yin
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Jiang
authors.forename: Jianhua
authors.initials: J
authors.lastname: Qin
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000254:growth & development*; D005947:Glucose / Q000737:chemistry; D005960:Glucosides / Q000378:metabolism*; D056656:Lab-On-A-Chip Devices; D008136:Longevity; D018384:Oxidative Stress; D013267:Stilbenes / Q000378:metabolism*
chemical_list: D005960:Glucosides; D013267:Stilbenes; D005947:Glucose; C058229:polydatin