title: Taxonomic dissection of Achromobacter denitrificans Coenye et al. 2003 and proposal of Achromobacter agilis sp. nov., nom. rev., Achromobacter pestifer sp. nov., nom. rev., Achromobacter kerstersii sp. nov. and Achromobacter deleyi sp. nov.
abstract: The phenotypic and genotypic characteristics of a historical collection of strains identified as Achromobacter denitrificanswere examined. Sequence analysis of a 765 bp nrdA gene fragment revealed that eight of these strains belonged to the recently described Achromobacter aegrifaciens, Achromobacter mucicolens, and Achromobacter insolitus, and that one strain belonged to Achromobacter xylosoxidans. The analysis also suggested the presence of four novel species of the genus Achromobacter among the remaining strains. The latter was confirmed by multilocus sequence analysis of concatenated nusA, eno, rpoB, gltB, lepA, nuoL andnrdA gene fragments and extensive genotypic and phenotypic characterization. We propose to name these novel species as Achromobacter agilis sp. nov., nom. rev. (type strain LMG 3411T=CCUG 62454T), Achromobacter pestifer sp. nov., nom. rev. (type strain LMG 3431T=CCUG 61959T) , Achromobacter kerstersii sp. nov. (type strain LMG 3441T=CCUG 62449T) and Achromobacter deleyi sp. nov. (type strain LMG 3458T=CCUG 62433T).
authors.affiliation: Laboratorium voor Microbiologie, Vakgroep Biochemie en microbiologie, Faculteit Wetenschappen, Universiteit Gent, Gent, Belgium.
authors.forename: Peter A
authors.initials: PA
authors.lastname: Vandamme
authors.affiliation: Laboratorium voor Microbiologie, Vakgroep Biochemie en microbiologie, Faculteit Wetenschappen, Universiteit Gent, Gent, Belgium.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Peeters
authors.affiliation: Department of Clinical Microbiology, Sahlgrenska Academy, Culture Collection niversity of Gothenburg, University of Gothenburg, Gothenburg, 41346, Sweden.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Inganäs
authors.affiliation: Laboratorium voor Microbiologie, Vakgroep Biochemie en microbiologie, Faculteit Wetenschappen, Universiteit Gent, Gent, Belgium.
authors.forename: Margo
authors.initials: M
authors.lastname: Cnockaert
authors.affiliation: Vakgroep Veterinaire Volksgezondheid en Voedselveiligheid, Faculteit Diergeneeskunde, Universiteit Gent, Gent, Belgium.
authors.forename: Kurt
authors.initials: K
authors.lastname: Houf
authors.affiliation: Department of Pediatrics and Communicable Disease, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
authors.forename: Theodore
authors.initials: T
authors.lastname: Spilker
authors.affiliation: Department of Clinical Microbiology, Sahlgrenska Academy, Culture Collection niversity of Gothenburg, University of Gothenburg, Gothenburg, 41346, Sweden.
authors.forename: Edward R B
authors.initials: ERB
authors.lastname: Moore
authors.affiliation: Department of Pediatrics and Communicable Disease, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
authors.forename: John J
authors.initials: JJ
authors.lastname: LiPuma
mesh_terms: D042402:Achromobacter / Q000145:classification*; D042441:Achromobacter denitrificans / Q000145:classification*; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D058885:Multilocus Sequence Typing; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial