title: Molecular characterization and expression pattern of a novel Keratin-associated protein 11.1 gene in the Liaoning cashmere goat (Capra hircus).
abstract: OBJECTIVE
  An experiment was conducted to determine the relationship between the KAP11.1 and the regulation wool fineness.
  
  
  METHODS
  In previous work, we constructed a skin cDNA library and isolated a full-length cDNA clone termedKAP11.1. On this basis, we conducted a series of bioinformatics analysis. Tissue distribution of KAP11.1 mRNA was performed using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) analysis. The expression of KAP11.1 mRNA in primary and secondary hair follicles was performed using real-time PCR (real-time polymerase chain reaction) analysis. The expression location of KAP11.1 mRNA in primary and secondary hair follicles was performed usingin situhybridization.
  
  
  RESULTS
  Bioinformatics analysis showed thatKAP11.1gene encodes a putative 158 amino acid protein that exhibited a high content of cysteine, serine, threonine, and valine and has a pubertal mammary gland) structural domain. Secondary structure prediction revealed a high proportion of random coils (76.73%). Semi-quantitative RT-PCR showed thatKAP11.1gene was expressed in heart, skin, and liver, but not expressed in spleen, lung and kidney. Real time PCR results showed that the expression ofKAP11.1has a higher expression in catagen than in anagen in the primary hair follicles. However, in the secondary hair follicles,KAP11.1has a significantly higher expression in anagen than in catagen. Moreover,KAP11.1gene has a strong expression in inner root sheath, hair matrix, and a lower expression in hair bulb.
  
  
  CONCLUSIONS
  We conclude thatKAP11.1gene may play an important role in regulating the fiber diameter.
authors.affiliation: Faculty of Life Science, Liaoning Normal University, Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery, Dalian 116081, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Jin
authors.affiliation: Faculty of Life Science, Liaoning Normal University, Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery, Dalian 116081, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Cao
authors.affiliation: Faculty of Life Science, Liaoning Normal University, Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery, Dalian 116081, China.
authors.forename: Ruilong
authors.initials: R
authors.lastname: Wang
authors.affiliation: Faculty of Life Science, Liaoning Normal University, Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery, Dalian 116081, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Piao
authors.affiliation: Faculty of Life Science, Liaoning Normal University, Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery, Dalian 116081, China.
authors.forename: Fengqin
authors.initials: F
authors.lastname: Zhao
authors.affiliation: Faculty of Life Science, Liaoning Normal University, Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery, Dalian 116081, China.
authors.forename: Jing'ai
authors.initials: J
authors.lastname: Piao
keywords: Expression Pattern; Gene; Hair; Keratin-associated Protein 11.1 (KAP11.1); Liaoning Cashmere Goat; Tissue-specific