title: Synthesis of photoactivatable azido-acyl caged oxazine fluorophores for live-cell imaging.
abstract: We report the design and synthesis of a photoactivatable azido-acyl oxazine fluorophore. Photoactivation is achieved cleanly and rapidly with UV light, producing a single fluorescent oxazine photoproduct. We demonstrate the utility of azido-acyl caged oxazines for protein specific labeling in living mammalian cells using the TMP-tag technology.
authors.affiliation: Department of Chemistry, Columbia University, New York, New York 10027, USA. ava2110@cumc.columbia.edu and Department of Systems Biology, Columbia University, New York, New York 10032, USA.
authors.forename: Andrew V
authors.initials: AV
authors.lastname: Anzalone
authors.affiliation: Department of Chemistry, Columbia University, New York, New York 10027, USA. ava2110@cumc.columbia.edu.
authors.forename: Zhixing
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Chemistry, Columbia University, New York, New York 10027, USA. ava2110@cumc.columbia.edu and Department of Systems Biology, Columbia University, New York, New York 10032, USA.
authors.forename: Virginia W
authors.initials: VW
authors.lastname: Cornish
mesh_terms: D000818:Animals; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D015394:Molecular Structure; D061848:Optical Imaging; D010078:Oxazines / Q000138:chemical synthesis* / Q000737:chemistry; D055668:Photochemical Processes
chemical_list: D005456:Fluorescent Dyes; D010078:Oxazines