title: [Diagnostic value of human papillomavirus E6/E7 mRNA for residue and recurrence after cervical conization].
abstract: OBJECTIVE
  To explore the diagnostic value of human papillomavirus E6/E7 mRNA for residue and recurrence of cervical intraepithelial neoplasia (CIN) patients after cervical conization.
  
  
  METHODS
  A total of 154 patients, who underwent cervical conization and possessed complete follow-up data of high-grade cervical intraepithelial neoplasia (CINII, III), were subjected to thin-prep cytology test (TCT), HPV-DNA typing and HPV E6/E7 mRNA test in 3-6 months and 12 months after cervical conization. Abnormal cytology (≥ASC-US) or high-risk HPV-DNA (+) or HPV E6/E7 mRNA (+) cases were found by colposcopy and cervical biopsy pathological diagnosis.
  
  
  RESULTS
  Nine patients had residue and 22 recurred. HPV-DNA detections after cervical conization in 57 patients were positive. Among them, 30 patients had residual/recurrent lesions. HPV E6/E7 mRNA detections in 26 patients were positive. Among them, 24 patients had residual/recurrent lesions. The sensitivity, specificity, positive predictive value, negative predictive value and accuracy of diagnosis in the detection of HPV-DNA were 96.8%, 78%, 52.6%, 99%, and 81.8%, respectively. The sensitivity, specificity, positive predictive value, negative predictive value and accuracy of diagnosis in the detection of HPV E6/E7 mRNA were 77.4%, 98.4%, 92.3%, 94.5%, and 94.2%, respectively. The specificity and positive predictive value of HPV E6/E7 mRNA test were greater than those of HPV-DNA typing test. There was a significant difference between the two groups (P<0.05).
  
  
  CONCLUSIONS
  Detection of HPV E6/E7 mRNA during the follow-up period could timely and effectively forecast the risk of residue and recurrence of CIN after cervical conization, and reduce excessive examination and treatment.
authors.affiliation: Medical School of Ningbo University, Ningbo Zhejiang 315000, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Obstetrics & Gynecology, Yangming Hospital Affiliated to Medical School of Ningbo University, Yuyao Zhejiang 315400, China.
authors.forename: Huajiang
authors.initials: H
authors.lastname: Shao
mesh_terms: D019092:Conization; D005260:Female; D006801:Humans; D009364:Neoplasm Recurrence, Local; D027383:Papillomaviridae; D030361:Papillomavirus Infections; D012333:RNA, Messenger; D012367:RNA, Viral; D002583:Uterine Cervical Neoplasms; D002578:Uterine Cervical Dysplasia
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral