title: GM-CSF and GM-CSF receptor have regulatory role in transforming rat mesenteric mesothelial cells into macrophage-like cells.
abstract: OBJECTIVE
  During peritonitis, mesothelial cells assume macrophage characteristics, expressing macrophage markers, indicating that they might differentiate into macrophage-like cells.
  
  
  METHODS
  Twenty-five male rats were used for in vivo experiments. For in vitro experiments, a primary mesentery culture model was developed. The mesothelial cell to macrophage-like cell transition was followed by studying ED1 expression.
  
  
  METHODS
  In vitro primary mesenteric culture was treated with granulocyte-macrophage colony-stimulating factor (GM-CSF, 1 ng/ml). Blocking internalization of receptor-ligand complex, Dynasore (80 µM) was used. Acute peritonitis was induced by Freund's adjuvant's (1 ml) intraperitoneal injection.
  
  
  RESULTS
  Immunohistochemistry: GM-CSF in vitro treatment resulted in a prominent ED1 expression in transformed mesothelial cells. Blocking the internalization, ED1 expression could not be detected. GM-CSF receptor (both α and β) was expressed in mesothelial cells in vitro (even if the GM-CSF was not present) and in vivo. Inflammation resulted in an increasing GM-CSF and GM-CSF-receptor level in the lysate of mesothelial cells.
  
  
  CONCLUSIONS
  Mesothelial cells can differentiate into macrophage-like cells, and GM-CSF, produced by the mesothelial cells, has probably an autocrine regulatory role in this transition. Our results provide new data about the plasticity of mesothelial cell and support the idea that during inflammation macrophages can derive from non-hematopoietic sources as well.
authors.affiliation: Department of Anatomy, Histology and Embryology, Semmelweis University, Tűzoltó u. 58., Budapest, 1094, Hungary.
authors.forename: Sándor
authors.initials: S
authors.lastname: Katz
authors.affiliation: Department of Anatomy, Histology and Embryology, Semmelweis University, Tűzoltó u. 58., Budapest, 1094, Hungary.
authors.forename: Viktória
authors.initials: V
authors.lastname: Zsiros
authors.affiliation: Department of Anatomy, Histology and Embryology, Semmelweis University, Tűzoltó u. 58., Budapest, 1094, Hungary.
authors.forename: Nikolett
authors.initials: N
authors.lastname: Dóczi
authors.affiliation: Department of Anatomy, Histology and Embryology, Semmelweis University, Tűzoltó u. 58., Budapest, 1094, Hungary.
authors.forename: Arnold
authors.initials: A
authors.lastname: Szabó
authors.affiliation: Department of Anatomy, Histology and Embryology, Semmelweis University, Tűzoltó u. 58., Budapest, 1094, Hungary.
authors.forename: Ádám
authors.initials: Á
authors.lastname: Biczó
authors.affiliation: Department of Anatomy, Histology and Embryology, Semmelweis University, Tűzoltó u. 58., Budapest, 1094, Hungary. kiss.anna@med.semmelweis-univ.hu.
authors.forename: Anna L
authors.initials: AL
authors.lastname: Kiss
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D005620:Freund's Adjuvant; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000166:cytology*; D008297:Male; D010538:Peritonitis / Q000139:chemically induced / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism*
chemical_list: D016187:Receptors, Granulocyte-Macrophage Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D005620:Freund's Adjuvant
keywords: GM-CSF; Mesothelial cell transformation; Peritoneal macrophages; Peritonitis