title: Correlation between radio-induced lymphocyte apoptosis measurements obtained from two French centres.
abstract: OBJECTIVE
  In the era of modern treatment delivery, increasing the dose delivered to the target to improve local control might be modulated by the patient's intrinsic radio-sensitivity. A predictive assay based on radio-induced lymphocyte apoptosis quantification highlighted the significant correlation between CD4 and CD8 T-lymphocyte apoptosis and grade 2 or 3 radiation-induced late toxicities. By conducting this assay at several technical platforms, the aim of this study was to demonstrate that radio-induced lymphocyte apoptosis values obtained from two different platforms were comparable.
  
  
  METHODS
  For 25 patients included in the PARATOXOR trial running in Dijon the radio-induced lymphocyte apoptosis results obtained from the laboratory of Montpellier (IRCM, Inserm U1194, France), considered as the reference (referred to as Lab 1), were compared with those from the laboratory located at the Institut de cancérologie de Lorraine (ICL, France), referred to as Lab 2. Different statistical methods were used to measure the agreement between the radio-induced lymphocyte apoptosis data from the two laboratories (quantitative data). The Bland-Altman plot was used to identify potential bias.
  
  
  RESULTS
  All statistical tests demonstrated good agreement between radio-induced lymphocyte apoptosis values obtained from both sites and no major bias was identified.
  
  
  CONCLUSIONS
  Since radio-induced lymphocyte apoptosis values, which predict tolerance to radiotherapy, could be assessed by two laboratories and showed a high level of robustness and consistency, we can suggest that this assay be extended to any laboratories that use the same technique.
authors.affiliation: Département de radiothérapie, centre Georges-François-Leclerc, 1, rue du Pr-Marion, 21079 Dijon cedex, France. Electronic address: cmirjolet@cgfl.fr.
authors.forename: C
authors.initials: C
authors.lastname: Mirjolet
authors.affiliation: Université de Lorraine, UMR7039 Cran, Campus Sciences, boulevard des Aiguillettes, BP 70239, 54506 Vandœuvre-lès-Nancy, France; CNRS UMR7039 Cran, Campus Sciences, boulevard des Aiguillettes, BP 70239, 54506 Vandœuvre-lès-Nancy, France; Département de biopathologie, institut de cancérologie de Lorraine, Nancy, France.
authors.forename: J L
authors.initials: JL
authors.lastname: Merlin
authors.affiliation: Département de biostatistiques, centre Georges-François-Leclerc, 1, rue du Pr-Marion, 21079 Dijon cedex, France.
authors.forename: C
authors.initials: C
authors.lastname: Dalban
authors.affiliation: Département de radiothérapie, centre Georges-François-Leclerc, 1, rue du Pr-Marion, 21079 Dijon cedex, France.
authors.forename: P
authors.initials: P
authors.lastname: Maingon
authors.affiliation: Pôle de radiothérapie oncologique, institut du cancer de Montpellier-Val d'Aurelle, 208, rue des Apothicaires, parc Euromédecine, 34298 Montpellier cedex 05, France; Inserm U1194 Immunociblage et radiobiologie en oncologie, 208, rue des Apothicaires, parc Euromédecine, 34298 Montpellier cedex 05, France.
authors.forename: D
authors.initials: D
authors.lastname: Azria
mesh_terms: D017209:Apoptosis; D005602:France; D006258:Head and Neck Neoplasms / Q000532:radiotherapy; D006801:Humans; D007755:Laboratories, Hospital / Q000592:standards*; D016014:Linear Models; D008214:Lymphocytes / Q000473:pathology*; D011446:Prospective Studies; D011878:Radiotherapy / Q000009:adverse effects*
keywords: Platform validation; RILA assay; Test de quantification de l’apoptose radio-induite des lymphocytes; Tolerance to radiotherapy; Tolérance à la radiothérapie; Validation de plateforme