title: Rapid identification of the NAT2 genotype in tuberculosis patients by multicolor melting curve analysis.
abstract: NAT2 genotype is an indicator for isoniazid dosage adjusting for tuberculosis treatment. Multicolor melting curve analysis (MMCA) was evaluated as a potential method for NAT2 genotyping.
  
  
  
  352 blood samples were analyzed by MMCA kit (Zeesan Biotech Co., Xiamen, China) targeting NAT2 SNPs at T341C, C481T, G590A and G857A, and direct sequencing was used as control.
  
  
  
  The sensitivity, specificity and accuracy of the MMCA assay for rapid NAT2 genotype detection were 97.9, 99.6 and 99.1% respectively, whereas for intermediate genotypes the values were 99.5, 98.7 and 99.1%, respectively, and for slow genotypes the values were 100% for the three aspects. The 24 saliva and blood for the control samples were also successfully analyzed using the MMCA assay, both produced uniform outcomes.
  
  
  
  The MMCA assay described in our study is very promising for the efficient determination of NAT2 genotype, and can facilitate the personalized dosing of isoniazid.
authors.affiliation: National Clinical Laboratory on Tuberculosis, Beijing Key laboratory on Drug-resistant Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis & Thoracic Tumor Institute, Beijing 101149, China.
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Hu
authors.affiliation: National Clinical Laboratory on Tuberculosis, Beijing Key laboratory on Drug-resistant Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis & Thoracic Tumor Institute, Beijing 101149, China.
authors.forename: Suting
authors.initials: S
authors.lastname: Chen
authors.affiliation: National Clinical Laboratory on Tuberculosis, Beijing Key laboratory on Drug-resistant Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis & Thoracic Tumor Institute, Beijing 101149, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Yu
authors.affiliation: National Clinical Laboratory on Tuberculosis, Beijing Key laboratory on Drug-resistant Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis & Thoracic Tumor Institute, Beijing 101149, China.
authors.forename: Guangming
authors.initials: G
authors.lastname: Dai
authors.affiliation: National Clinical Laboratory on Tuberculosis, Beijing Key laboratory on Drug-resistant Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis & Thoracic Tumor Institute, Beijing 101149, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Dong
authors.affiliation: National Clinical Laboratory on Tuberculosis, Beijing Key laboratory on Drug-resistant Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis & Thoracic Tumor Institute, Beijing 101149, China.
authors.forename: Yunxu
authors.initials: Y
authors.lastname: Li
authors.affiliation: National Clinical Laboratory on Tuberculosis, Beijing Key laboratory on Drug-resistant Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis & Thoracic Tumor Institute, Beijing 101149, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Zhao
authors.affiliation: National Clinical Laboratory on Tuberculosis, Beijing Key laboratory on Drug-resistant Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis & Thoracic Tumor Institute, Beijing 101149, China.
authors.forename: Hairong
authors.initials: H
authors.lastname: Huang
mesh_terms: D000107:Acetylation; D000995:Antitubercular Agents / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000627:therapeutic use; D001191:Arylamine N-Acetyltransferase / Q000097:blood / Q000235:genetics*; D002681:China; D005838:Genotype; D006801:Humans; D007538:Isoniazid / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000627:therapeutic use; D007700:Kinetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D057285:Precision Medicine; D012463:Saliva / Q000737:chemistry; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000235:genetics*
chemical_list: D000995:Antitubercular Agents; D001191:Arylamine N-Acetyltransferase; C478900:NAT2 protein, human; D007538:Isoniazid
keywords: NAT2; acetylator genotype; multicolor melting curve analysis