title: Strategy study on enhancing lipid productivity of filamentous oleaginous microalgae Tribonema.
abstract: Filamentous microalgae Tribonema is promising for biodiesel production in terms of high lipid content, easy harvesting and good contamination tolerance. As high lipid productivity is a key basis, several methods including different culture medium, phosphorus, iron and CO2 inductions were tested to enhance lipid productivity in Tribonema minus. T. minus subjected to BG11-freshwater medium achieved a favorable lipid productivity of 216.18mgL(-1)d(-1), much higher than those in other culture medium (BG11-S, Kuhl, f/2, f, 2f, BBM, ASW and ES). Secondly, the optimal phosphorus, iron and CO2 concentrations for high lipid productivity of T. minus were obtained, respectively. Finally, the cells of T. minus grown in optimized BG11 medium containing 80mgL(-1) KH2PO4 and 6mgL(-1) FeC6H5O7·NH4OH with 2% CO2 achieved a lipid productivity of 384.67mgL(-1)d(-1), representing a 1.56-fold increase from the control. Therefore, the strategy to enhance lipid productivity of T. minus was successfully proposed.
authors.affiliation: CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, PR China.
authors.forename: Wang
authors.initials: W
authors.lastname: Hui
authors.affiliation: CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, PR China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Wenjun
authors.affiliation: CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, PR China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Wentao
authors.affiliation: CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, PR China.
authors.forename: Gao
authors.initials: G
authors.lastname: Lili
authors.affiliation: CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, PR China. Electronic address: liutz@qibebt.ac.cn.
authors.forename: Liu
authors.initials: L
authors.lastname: Tianzhong
mesh_terms: D056804:Biofuels; D018533:Biomass; D019149:Bioreactors; D002245:Carbon Dioxide / Q000737:chemistry; D003470:Culture Media / Q000737:chemistry; D007501:Iron / Q000737:chemistry; D008055:Lipids / Q000096:biosynthesis* / Q000737:chemistry; D058086:Microalgae / Q000378:metabolism*; D010758:Phosphorus / Q000737:chemistry; D058009:Stramenopiles / Q000378:metabolism*
chemical_list: D056804:Biofuels; D003470:Culture Media; D008055:Lipids; D002245:Carbon Dioxide; D010758:Phosphorus; D007501:Iron
keywords: CO(2) concentration; Iron ion; Lipid productivity; Phosphorus; Tribonema