title: [Gene deletion and functional analysis of the heptyl glycosyltransferase (waaF) gene in Vibrio parahemolyticus O-antigen cluster].
abstract: OBJECTIVE
  To construct heptyl glycosyltransferase gene II (waaF) gene deletion mutant of Vibrio parahaemolyticus, and explore the function of the waaF gene in Vibrio parahaemolyticus.
  
  
  METHODS
  The waaF gene deletion mutant was constructed by chitin-based transformation technology using clinical isolates, and then the growth rate, morphology and serotypes were identified. The different sources (O3, O5 and O10) waaF gene complementations were constructed through E. coli S17λpir strains conjugative transferring with Vibrio parahaemolyticus, and the function of the waaF gene was further verified by serotypes.
  
  
  RESULTS
  The waaF gene deletion mutant strain was successfully constructed and it grew normally. The growth rate and morphology of mutant were similar with the wild type strains (WT), but the mutant could not occurred agglutination reaction with O antisera. The O3 and O5 sources waaF gene complementations occurred agglutination reaction with O antisera, but the O10 sources waaF gene complementations was not.
  
  
  CONCLUSIONS
  The waaF gene was related with O-antigen synthesis and it was the key gene of O-antigen synthesis pathway in Vibrio parahaemolyticus. The function of different sources waaF gene were not the same.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhao
authors.forename: Songsong
authors.initials: S
authors.lastname: Meng
authors.forename: Deqing
authors.initials: D
authors.lastname: Zhou
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D017353:Gene Deletion; D016695:Glycosyltransferases / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D005810:Multigene Family; D019081:O Antigens / Q000096:biosynthesis / Q000235:genetics; D014735:Vibrio Infections / Q000382:microbiology; D014736:Vibrio parahaemolyticus / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development
chemical_list: D001426:Bacterial Proteins; D019081:O Antigens; D016695:Glycosyltransferases