title: Circulating miR-221-3p as a novel marker for early prediction of acute myocardial infarction.
abstract: Recent studies have reported circulating microRNAs (miRNAs) as novel biomarkers for cardiovascular diseases including acute myocardial infarction, heart failure, diabetes mellitus, stroke, and acute pulmonary embolism. The aims of this study were 1) to compare the plasma expression levels of miRNAs in patients with acute coronary syndrome (ACS) and control subjects and in ST-elevation myocardial infarction (STEMI) and non-STEMI 2) to evaluate miRNAs potential to be used as novel diagnostic biomarkers for ACS. Twenty seven consecutive patients, admitted to emergency department of a training and research hospital between January-December 2013 with acute chest pain and/or dyspnea and diagnosed with ACS, and 16 non-ACS control subjects were included in this study. miRNA profiling was performed by using real time polymerase chain reaction. Functions of dysregulated miRNAs were evaluated by computerized-pathways analysis. miR-221-3p was one of the two most dysregulated miRNAs with a fold regulation of 3.89. It was significantly positively correlated with both Troponin and GRACE and Synthax Score. Moreover, miR221-3p was found to be significantly inversely correlated with left ventricular ejection fraction. miR-221-3p was the most prominent biomarker candidate with an area under curve (AUC) level of 0.881 (95% confidence interval: 0.774-0.987; p=0.002). The present study is the first to report an increased expression levels of miR-221-3p in AMI. Since miR-221-3p has a high discriminative value and significant relations with Troponin, GRACE and Synthax score and left ventricular systolic function, it may be a potential biomarker for early prediction of AMI.
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Saglik Bilimleri University, Istanbul, Turkey. Electronic address: ecoskunpinar@gmail.com.
authors.forename: Ender
authors.initials: E
authors.lastname: Coskunpinar
authors.affiliation: Mustafakemalpasa State Hospital, Department of Cardiology, Bursa, Turkey.
authors.forename: Huseyin Altug
authors.initials: HA
authors.lastname: Cakmak
authors.affiliation: Mehmet Akif Ersoy Thoracic and Cardiovascular Surgery Training and Research Hospital, Department of Cardiology, Istanbul, Turkey.
authors.forename: Ali Kemal
authors.initials: AK
authors.lastname: Kalkan
authors.affiliation: Department of Molecular Medicine, Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey.
authors.forename: Necip Ozan
authors.initials: NO
authors.lastname: Tiryakioglu
authors.affiliation: Mehmet Akif Ersoy Thoracic and Cardiovascular Surgery Training and Research Hospital, Department of Cardiology, Istanbul, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Erturk
authors.affiliation: Cerrahpasa School of Medicine, Department of Cardiology, Istanbul University, Istanbul, Turkey.
authors.forename: Zeki
authors.initials: Z
authors.lastname: Ongen
mesh_terms: D054058:Acute Coronary Syndrome / Q000097:blood / Q000235:genetics; D015415:Biomarkers / Q000378:metabolism; D003710:Demography; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics*; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood / Q000175:diagnosis* / Q000235:genetics*; D012372:ROC Curve; D015203:Reproducibility of Results; D015398:Signal Transduction / Q000235:genetics
chemical_list: D015415:Biomarkers; D035683:MicroRNAs; C525709:MIR221, human
keywords: Acute myocardial infarction; Biomarker; Diagnosis; miR-221-3p