title: A Calcium-Ion-Stabilized Lipase from Pseudomonas stutzeri ZS04 and its Application in Resolution of Chiral Aryl Alcohols.
abstract: An extracellular organic solvent-tolerant lipase-producing bacterium was isolated from oil-contaminated soil samples and was identified taxonomically as Pseudomonas stutzeri, from which the lipase was purified and exhibited maximal activity at temperature of 50 °C and pH of 9.0. Meanwhile, the lipase was stable below or at 30 °C and over an alkaline pH range (7.5-11.0). Ca2+could significantly improve the lipase thermal stability which prompts a promising application in biocatalysis through convenient medium engineering. The lipase demonstrated striking features such as distinct stability to the most tested hydrophilic and hydrophobic solvents (25 %, v/v), and DMSO could activate the lipase dramatically. In the enzyme-catalyzed resolution, lipase ZS04 manifested excellent enantioselective esterification toward the (R)-1-(4-methoxyphenyl)-ethanol (MOPE), a crucial chiral intermediate in pharmaceuticals as well as in other analogs with strict substrate specificity and theoretical highest conversion yield. This strong advantage over other related schemes made lipase ZS04 a promising biocatalyst in organic synthesis and pharmaceutical applications.
authors.affiliation: Pharmaceutical College, Nanjing Technology University, 5 Xinmofan Road, Nanjing, 210009, China.
authors.forename: Song
authors.initials: S
authors.lastname: Qin
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Technology University, 5 Xinmofan Road, Nanjing, 210009, China.|Innovent Biologics Co. Ltd., 168 Dongping Street, Suzhou, 215123, China.
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Technology University, 5 Xinmofan Road, Nanjing, 210009, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
authors.affiliation: Pharmaceutical College, Nanjing Technology University, 5 Xinmofan Road, Nanjing, 210009, China. bingfanghe@njtech.edu.cn.|College of Biotechnology and Pharmaceutical Engineering, Nanjing Technology University, 5 Xinmofan Road, Nanjing, 210009, China. bingfanghe@njtech.edu.cn.
authors.forename: Bingfang
authors.initials: B
authors.lastname: He
mesh_terms: D000438:Alcohols / Q000737:chemistry*; D002118:Calcium / Q000494:pharmacology*; D003902:Detergents / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004795:Enzyme Stability / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007477:Ions; D008049:Lipase / Q000302:isolation & purification / Q000378:metabolism*; D044223:Pseudomonas stutzeri / Q000187:drug effects / Q000201:enzymology* / Q000302:isolation & purification; D012997:Solvents; D013379:Substrate Specificity / Q000187:drug effects; D013696:Temperature
chemical_list: D000438:Alcohols; D003902:Detergents; D004791:Enzyme Inhibitors; D007477:Ions; D012997:Solvents; D008049:Lipase; D002118:Calcium
keywords: Chiral resolution; Lipase; MOPE; Organic solvent stability; Pseudomonas stutzeri