title: Expression of p40 (∆Np63) protein in meningiomas, an unexpected finding: immunohistochemical study and evaluation of its possible prognostic role.
abstract: According to the 2007 WHO (World Health Organization) Classification, meningiomas are divided into three grades of malignancy, with different recurrence rate, based exclusively on histopathological parameters. Loss/reduction of PgR (Progesterone Receptor) expression and increased Ki67 L.I. (Labeling Index) have been proven as possible prognostic factors able to predict the relapse of the disease. However, they sometimes result unreliable, especially when discordant. p40 is the short form of the p53 homologue gene p63, also named ∆Np63, and its antibody has recently been introduced as a highly specific diagnostic marker of the squamous cell carcinoma of the lung. Nevertheless its expression has been found in many other unconventional sites (e.g. placenta, urotheluim, etc). Herein we assessed the immuno-expression of p40 protein in a series of 72 meningiomas (35 grade I and 37 grade II) and analyzed its correlation with clinicopathological parameters, overall survival and recurrence free interval. We found that a high p40 score correlated with high histological grade, presence of recurrence, increased Ki67 L.I. and loss/reduction of PgR signal. Moreover, a higher expression of p40 was shown to be a significant prognostic factor for the development of recurrences and resulted a prognostic independent variable in multivariate analysis. Overall, for the first time, we investigated the expression of p40 protein in meningiomas and explored its usefulness as prognostic marker in addition to PgR and Ki67 L.I.
authors.affiliation: Pathology Section, Department of Advanced Biomedical Sciences, University of Naples Federico II, Via Pansini 5, 80131, Naples, Italy. eliaguadagno84@gmail.com.
authors.forename: Elia
authors.initials: E
authors.lastname: Guadagno
authors.affiliation: Pathology Section, Department of Advanced Biomedical Sciences, University of Naples Federico II, Via Pansini 5, 80131, Naples, Italy.
authors.forename: Marialaura
authors.initials: M
authors.lastname: Del Basso De Caro
authors.affiliation: Pathology Section, Department of Advanced Biomedical Sciences, University of Naples Federico II, Via Pansini 5, 80131, Naples, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Pignatiello
authors.affiliation: Department of Clinical Medicine and Surgery, University of Naples Federico II, Via Pansini 5, 80131, Naples, Italy.
authors.forename: Concetta
authors.initials: C
authors.lastname: Sciammarella
authors.affiliation: Division of Neurosurgery, Department of Neurosciences, Reproductive and Odontostomatological Sciences, University of Naples Federico II, Via Pansini 5, 80131, Naples, Italy.
authors.forename: Domenico
authors.initials: D
authors.lastname: Solari
authors.affiliation: Division of Neurosurgery, Department of Neurosciences, Reproductive and Odontostomatological Sciences, University of Naples Federico II, Via Pansini 5, 80131, Naples, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Cappabianca
authors.affiliation: Division of Neurosurgery, Department of Neurosciences, Reproductive and Odontostomatological Sciences, University of Naples Federico II, Via Pansini 5, 80131, Naples, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Maiuri
authors.affiliation: Division of Neurosurgery, Department of Neurosciences, Reproductive and Odontostomatological Sciences, University of Naples Federico II, Via Pansini 5, 80131, Naples, Italy.
authors.forename: Flavia
authors.initials: F
authors.lastname: Dones
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D008577:Meningeal Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000401:mortality; D008579:Meningioma / Q000175:diagnosis / Q000378:metabolism* / Q000401:mortality; D008875:Middle Aged; D011379:Prognosis; D011980:Receptors, Progesterone / Q000032:analysis; D018709:Statistics, Nonparametric; D014157:Transcription Factors / Q000032:analysis* / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000032:analysis* / Q000378:metabolism; D055815:Young Adult
chemical_list: C081876:CKAP4 protein, human; D019394:Ki-67 Antigen; D008565:Membrane Proteins; D011980:Receptors, Progesterone; C115051:TP63 protein, human; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins
keywords: Meningioma; P40; Prognostic marker; ∆Np63