title: The transcriptomes of novel marmoset monkey embryonic stem cell lines reflect distinct genomic features.
abstract: Embryonic stem cells (ESCs) are useful for the study of embryonic development. However, since research on naturally conceived human embryos is limited, non-human primate (NHP) embryos and NHP ESCs represent an excellent alternative to the corresponding human entities. Though, ESC lines derived from naturally conceived NHP embryos are still very rare. Here, we report the generation and characterization of four novel ESC lines derived from natural preimplantation embryos of the common marmoset monkey (Callithrix jacchus). For the first time we document derivation of NHP ESCs derived from morula stages. We show that quantitative chromosome-wise transcriptome analyses precisely reflect trisomies present in both morula-derived ESC lines. We also demonstrate that the female ESC lines exhibit different states of X-inactivation which is impressively reflected by the abundance of the lncRNA X inactive-specific transcript (XIST). The novel marmoset ESC lines will promote basic primate embryo and ESC studies as well as preclinical testing of ESC-based regenerative approaches in NHP.
authors.affiliation: Platform Degenerative Diseases, German Primate Center - Leibniz Institute for Primate Research, Kellnerweg 4, 37077 Göttingen, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Debowski
authors.affiliation: Platform Degenerative Diseases, German Primate Center - Leibniz Institute for Primate Research, Kellnerweg 4, 37077 Göttingen, Germany.
authors.forename: Charis
authors.initials: C
authors.lastname: Drummer
authors.affiliation: Platform Degenerative Diseases, German Primate Center - Leibniz Institute for Primate Research, Kellnerweg 4, 37077 Göttingen, Germany.
authors.forename: Jana
authors.initials: J
authors.lastname: Lentes
authors.affiliation: Platform Degenerative Diseases, German Primate Center - Leibniz Institute for Primate Research, Kellnerweg 4, 37077 Göttingen, Germany.
authors.forename: Maren
authors.initials: M
authors.lastname: Cors
authors.affiliation: Institute of Cellular and Molecular Immunology, University Medical Center Göttingen (UMG), Humboldtallee 34, 37073 Göttingen, Germany.|DZHK (German Center for Cardiovascular Research), Partner Site Göttingen, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Dressel
authors.affiliation: Microarray and Deep-Sequencing Core Facility, University Medical Center Göttingen (UMG), Justus-von-Liebig-Weg 11, 37077 Göttingen, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Lingner
authors.affiliation: Microarray and Deep-Sequencing Core Facility, University Medical Center Göttingen (UMG), Justus-von-Liebig-Weg 11, 37077 Göttingen, Germany.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Salinas-Riester
authors.affiliation: Department of Human Genetics, University Medical Center Hamburg-Eppendorf, Martinistraße 52, 20246 Hamburg, Germany.
authors.forename: Sigrid
authors.initials: S
authors.lastname: Fuchs
authors.affiliation: Department of Applied Developmental Biology, Central Institute for Experimental Animals, 3-25-12 Tonomachi Kawasaki-ku, Kawasaki, 210-0821 Japan.|Keio Advanced Research Center, Keio University, Shinjuku-ku, Tokyo, Japan.
authors.forename: Erika
authors.initials: E
authors.lastname: Sasaki
authors.affiliation: Platform Degenerative Diseases, German Primate Center - Leibniz Institute for Primate Research, Kellnerweg 4, 37077 Göttingen, Germany.|DZHK (German Center for Cardiovascular Research), Partner Site Göttingen, Germany.
authors.forename: Rüdiger
authors.initials: R
authors.lastname: Behr
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001755:Blastocyst / Q000166:cytology; D002144:Callithrix / Q000235:genetics*; D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D048430:Cell Shape; D053595:Embryonic Stem Cells / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D016678:Genome; D007621:Karyotyping; D008297:Male; D009028:Morula / Q000166:cytology; D019849:Sex Determination Processes / Q000235:genetics; D013724:Teratoma / Q000473:pathology; D059467:Transcriptome / Q000235:genetics*; D049951:X Chromosome Inactivation / Q000235:genetics
chemical_list: D015415:Biomarkers