title: PLAG1 expression is maintained in recurrent pleomorphic adenoma.
abstract: The proto-oncogene (pleomorphic adenoma gene 1 (PLAG1)) is immunohistochemically overexpressed in pleomorphic adenoma (PA). Its expression in recurrent pleomorphic adenoma (RPA), however, has not been investigated. Since complex mechanisms are involved in tumor recurrence, the aim of this study was to investigate whether PLAG1 overexpression occurs in RPA. We studied PLAG1 protein expression in 40 PAs and 36 RPAs by immunohistochemistry. Cases with immunopositive cells were classified into two categories, between 10 and 50 % and >50 %. In both groups, PLAG1 expression was observed in both epithelial and myoepithelial cells. Of PAs, 37 cases (93 %) were positive, while this was the case in 34 RPA cases (94 %). Our findings suggest that in addition to morphological similarity, PA and RPA express PLAG1, which might play a role in tumor recurrence. Furthermore, as for PA, expression of PLAG1 can be considered a valuable diagnostic marker for RPA.
authors.affiliation: Pathology Department, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Beatriz Samara
authors.initials: BS
authors.lastname: de Brito
authors.affiliation: Pathology Department, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Natália Giovanelli
authors.initials: NG
authors.lastname: Gaspar
authors.affiliation: Pathology Department, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Erika Said Abu
authors.initials: ES
authors.lastname: Egal
authors.affiliation: Oral Pathology Department, Piracicaba Dental School, State University of Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Celeste
authors.initials: C
authors.lastname: Sanchez-Romero
authors.affiliation: Head and Neck Surgery Department, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Antonio Santos
authors.initials: AS
authors.lastname: Martins
authors.affiliation: Head and Neck Surgery Department, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Álfio José
authors.initials: ÁJ
authors.lastname: Tincani
authors.affiliation: Pathology Department, Federal University of Santa Catarina (UFSC), Florianópolis, SC, Brazil.
authors.forename: Rogério
authors.initials: R
authors.lastname: de Oliveira Gondak
authors.affiliation: Oral Pathology Department, Piracicaba Dental School, State University of Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Oslei Paes
authors.initials: OP
authors.lastname: de Almeida
authors.affiliation: Head and Neck Surgery Department, AC Camargo Cancer Center, São Paulo, SP, Brazil.
authors.forename: Luiz Paulo
authors.initials: LP
authors.lastname: Kowalski
authors.affiliation: Pathology Department, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, SP, Brazil.
authors.forename: Albina
authors.initials: A
authors.lastname: Altemani
authors.affiliation: Pathology Department, Faculty of Medical Sciences, State University of Campinas (UNICAMP), Campinas, SP, Brazil. fevimariano@gmail.com.|Departamento de Anatomia Patológica, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, Av. Tessália Vieira de Camargo, 126 - Barão Geraldo, Cidade Universitária, Campinas, SP, 13083-887, Brazil. fevimariano@gmail.com.
authors.forename: Fernanda Viviane
authors.initials: FV
authors.lastname: Mariano
mesh_terms: D008949:Adenoma, Pleomorphic / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology*; D000328:Adult; D000368:Aged; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008297:Male; D008875:Middle Aged; D000090063:Proto-Oncogene Mas; D012008:Recurrence; D012468:Salivary Gland Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D014157:Transcription Factors / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; C000715470:MAS1 protein, human; C104382:PLAG1 protein, human; D000090063:Proto-Oncogene Mas; D014157:Transcription Factors
keywords: Immunohistochemistry; PLAG1; Pleomorphic adenoma; Recurrent pleomorphic adenoma