title: Influence of co-culture on osteogenesis and angiogenesis of bone marrow mesenchymal stem cells and aortic endothelial cells.
abstract: Co-culture of bone forming cells and endothelial cells to induce pre-vascularization is one of the strategies used to solve the insufficient vascularization problem in bone tissue engineering attempts. In the study, primary cells isolated from 2 different tissues of the same animal, rat bone marrow stem cells (RBMSCs) and rat aortic endothelial cells (RAECs) were co-cultured to study the effects of co-culturing on both osteogenesis and angiogenesis. The formation of tube like structure in 2D culture was observed for the first time in the literature by the co-culture of primary cells from the same animal and also osteogenesis and angiogenesis were investigated at the same time by using this co-culture system. Co-cultured cells mineralized and formed microvasculature beginning from 14days of incubation. After 28days of incubation in the osteogenic medium, expression of osteogenic genes in co-cultures was significantly upregulated compared to RBMSCs cultured alone. These results suggest that the co-culture of endothelial cells with mesenchymal stem cells induces both osteogenesis and angiogenesis.
authors.affiliation: Genetics and Bioengineering Department, Yeditepe University, Istanbul, Turkey; Molecular Biology - Genetics and Biotechnology Department, Istanbul Technical University, Istanbul, Turkey; Center of Excellence in Biomaterials and Tissue Engineering, BIOMATEN, METU, Ankara, Turkey.
authors.forename: Gorke
authors.initials: G
authors.lastname: Gurel Pekozer
authors.affiliation: Genetics and Bioengineering Department, Yeditepe University, Istanbul, Turkey; Center of Excellence in Biomaterials and Tissue Engineering, BIOMATEN, METU, Ankara, Turkey. Electronic address: gamzekose@yeditepe.edu.tr.
authors.forename: Gamze
authors.initials: G
authors.lastname: Torun Kose
authors.affiliation: Department of Biological Sciences, Biotechnology Research Unit, METU, BIOMAT, Ankara, Turkey; Center of Excellence in Biomaterials and Tissue Engineering, BIOMATEN, METU, Ankara, Turkey.
authors.forename: Vasif
authors.initials: V
authors.lastname: Hasirci
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001011:Aorta / Q000166:cytology*; D001854:Bone Marrow Cells / Q000378:metabolism / Q000502:physiology*; D001857:Bone Matrix / Q000378:metabolism; D002113:Calcification, Physiologic; D002450:Cell Communication; D002454:Cell Differentiation / Q000235:genetics; D019070:Cell Lineage; D049109:Cell Proliferation; D002478:Cells, Cultured; D018920:Coculture Techniques; D042783:Endothelial Cells / Q000378:metabolism / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000502:physiology*; D018919:Neovascularization, Physiologic; D010012:Osteogenesis / Q000235:genetics; D010641:Phenotype; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D055153:Stem Cell Niche; D013997:Time Factors
chemical_list: D000469:Alkaline Phosphatase
keywords: Bone tissue engineering; Co-culture; Endothelial cells; Mesenchymal stem cells; Vascularization