title: A phenazine-1-carboxylic acid producing polyextremophilic Pseudomonas chlororaphis (MCC2693) strain, isolated from mountain ecosystem, possesses biocontrol and plant growth promotion abilities.
abstract: The genus Pseudomonas is known to comprise a huge diversity of species with the ability to thrive in different habitats, including those considered as extreme environments. In the present study, a psychrotolerant, wide pH tolerant and halotolerant strain of Pseudomonas chlororaphis GBPI_507 (MCC2693), isolated from the wheat rhizosphere growing in a mountain location in Indian Himalayan Region (IHR), has been investigated for its antimicrobial potential with particular reference to phenazine production and plant growth promoting traits. GBPI_507 showed phenazine production at the temperatures ranged from 14 to 25°C. The benzene extracted compound identified as phenazine-1-carboxylic acid (PCA) through GC-MS exhibited antimicrobial properties against Gram positive bacteria and actinomycetes. The inhibition of phytopathogens in diffusible biocontrol assays was recorded in an order: Alternaria alternata>Phytophthora sp.>Fusarium solani>F. oxysporum. In volatile metabolite assays, all the pathogens, except Phytophthora sp. produced distorted colonies, characterized by restricted sporulation. The isolate also possessed other growth promoting and biocontrol traits including phosphate solubilization and production of siderophores, HCN, ammonia, and lytic enzymes (lipase and protease). Molecular studies confirmed production of PCA by the bacterium GBPI_507 through presence of phzCD and phzE genes in its genome. The polyextremophilic bacterial strain possesses various important characters to consider it as a potential agent for field applications, especially in mountain ecosystem, for sustainable and eco-friendly crop production.
authors.affiliation: Biotechnological Applications, G.B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora, 263 643, Uttarakhand, India.
authors.forename: Rahul
authors.initials: R
authors.lastname: Jain
authors.affiliation: Biotechnological Applications, G.B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora, 263 643, Uttarakhand, India. Electronic address: anita@gbpihed.nic.in.
authors.forename: Anita
authors.initials: A
authors.lastname: Pandey
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004798:Enzymes / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006094:Gram-Positive Bacteria / Q000187:drug effects / Q000254:growth & development; D006856:Hydrogen Cyanide / Q000378:metabolism; D007194:India; D008826:Microbial Sensitivity Tests; D010619:Phenazines / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D010802:Phylogeny; D063245:Plant Development / Q000187:drug effects*; D010937:Plant Growth Regulators / Q000378:metabolism*; D000070096:Pseudomonas chlororaphis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D017262:Siderophores / Q000378:metabolism; D012988:Soil Microbiology; D013696:Temperature; D014908:Triticum / Q000254:growth & development / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D004798:Enzymes; D010619:Phenazines; D010710:Phosphates; D010937:Plant Growth Regulators; D012336:RNA, Ribosomal, 16S; D017262:Siderophores; C037165:1-phenazinecarboxylic acid; D006856:Hydrogen Cyanide
keywords: Biocontrol; Indian Himalayan Region; Phenazine; Plant growth; Pseudomonas