title: The Relationship Between Glutathione S-Transferase-P1 and Beta-2 Adrenoreceptor Genotypes with Asthmatic Patients in the Turkish Population.
abstract: BACKGROUND
  Individual differences in the activity of enzymes that metabolize xenobiotics can impact health and disease. Beta-2 adrenoreceptor (ADRB2) is a functional G-coupled protein expressed in the vascular endothelium of lungs, alveolar walls, and the ganglions of cholinergic nerves which induces bronchodilation in response to catecholamines. Glutathione S-Transferase-P1 (GSTP1) is a candidate pi class GST gene, which controls pi class glutathione S-transferase activity.
  
  
  OBJECTIVE
  In this study we determined the relationship between the ADRB2 Arg16Gly polymorphism and GSTP1 polymorphisms, involved in bronchodilator response and oxidative stress, respectively, with susceptibility to asthma.
  
  
  METHODS
  In this study, 129 asthmatic patients and 127 healthy control cases were recruited to determine ADRB2 and GSTP1 genotypes by allele-specific polymerase chain reaction and restriction fragment length polymorphism assays, respectively.
  
  
  RESULTS
  The ADRB2 genotype frequencies of the patients and control cases were found to be 10.9% (Arg16Arg), 48.8% (Arg16Gly), and 40.3% (Gly16Gly) and 24.4% (Arg16Arg), 36.2% (Arg16Gly), and 39.4% (Gly16Gly), respectively. GSTP1 genotype frequencies of patients and control cases were found to be 55% (Ile105Ile), 43.4% (Ile105Val), and 1.6% (Val105Val) and 75.6% (Ile105Ile), 22% (Ile105Val), and 2.4% (Val105Val), respectively. In the case of the GSTP1 gene, we found statistically significant differences in the genotype frequency of Ile105Val and the allele frequency of Val105 in the asthmatic group compared with the controls. Moreover, we observed a relationship between allele frequencies and clinical phenotypes including atopia nocturnal dyspnea, and steroid dependency in the asthmatic patients.
  
  
  CONCLUSIONS
  Our results suggest that the GSTP1 Ile105Val polymorphism may be linked to the severeness of airway dysfunction.
authors.affiliation: 1 Anesthesiology and Reanimation Department, Ministry of Health, Intensive Care Unit, Ankara Training and Research Hospital , Ankara, Turkey .
authors.forename: Cetin
authors.initials: C
authors.lastname: Kaymak
authors.affiliation: 2 Department of Toxicology, Faculty of Pharmacy, Gazi University , Ankara, Turkey .
authors.forename: Neslihan
authors.initials: N
authors.lastname: Aygun Kocabas
authors.affiliation: 3 Department of Respiratory Disease, Ministry of Health, Ankara Training and Research Hospital , Ankara, Turkey .
authors.forename: Nesrin
authors.initials: N
authors.lastname: Aydın
authors.affiliation: 4 Department of Medical Biostatistics, Faculty of Medicine, University of Ankara , Ankara, Turkey .
authors.forename: Derya
authors.initials: D
authors.lastname: Oztuna
authors.affiliation: 2 Department of Toxicology, Faculty of Pharmacy, Gazi University , Ankara, Turkey .
authors.forename: Ali Esat
authors.initials: AE
authors.lastname: Karakaya
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D001249:Asthma / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D051549:Glutathione S-Transferase pi / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D018343:Receptors, Adrenergic, beta-2 / Q000235:genetics* / Q000378:metabolism; D014421:Turkey
chemical_list: C553443:ADRB2 protein, human; D018343:Receptors, Adrenergic, beta-2; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi