title: Study of surface carbohydrates in Galba truncatula tissues before and after infection with Fasciola hepatica.
abstract: The presence and distribution of surface carbohydrates in the tissues of Galba truncatula snails uninfected or after infection with Fasciola hepatica as well as on the surface of the snail-pathogenic larval stages of the parasite were studied by lectin labelling assay. This is an attempt to find similarities that indicate possible mimicry, utilised by the parasite as an evasion strategy in this snail-trematode system. Different binding patterns were identified on head-foot-mantle, hepatopancreas, genital glands, renopericardial complex of the host as well as of the snail-pathogenic larval stages of F. hepatica. The infection with F. hepatica leads to changes of labelling with Glycine max in the head-mantle cells and Arachis hypogaea in the tubular epithelium of the hepatopancreas. The lectin binding on the other snail tissues is not changed by the development of the larvae. Our data clearly demonstrated the similarity in labelling of G. truncatula tissues and the surface of the snail-pathogenic larval stages of F. hepatica. The role of glycosylation of the contact surfaces of both organisms in relation to the host-parasite interactions is also discussed.
authors.affiliation: Bulgarian Academy of Sciences, Institute of Biodiversity and Ecosystem Research, Department of Animal Diversity and Resources, Sofia, Bulgaria.
authors.forename: Katya
authors.initials: K
authors.lastname: Georgieva
authors.affiliation: Bulgarian Academy of Sciences, Institute of Experimental Morphology, Pathology and Anthropology with Museum, Sofia, Bulgaria.
authors.forename: Liliya
authors.initials: L
authors.lastname: Georgieva
authors.affiliation: Bulgarian Academy of Sciences, Institute of Biodiversity and Ecosystem Research, Department of Animal Diversity and Resources, Sofia, Bulgaria.
authors.forename: Yana
authors.initials: Y
authors.lastname: Mizinska-Boevska
authors.affiliation: Bulgarian Academy of Sciences, Institute of Microbiology, Sofia, Bulgaria.
authors.forename: Stoyanka R
authors.initials: SR
authors.lastname: Stoitsova
mesh_terms: D000818:Animals; D010367:Arachis; D002241:Carbohydrates / Q000502:physiology*; D005210:Fasciola hepatica / Q000378:metabolism* / Q000469:parasitology; D005211:Fascioliasis / Q000378:metabolism* / Q000469:parasitology; D006031:Glycosylation; D007814:Larva / Q000378:metabolism / Q000469:parasitology; D037102:Lectins / Q000378:metabolism*; D008195:Lymnaea / Q000378:metabolism* / Q000469:parasitology; D008856:Microscopy, Fluorescence; D033661:Oocysts / Q000469:parasitology; D012016:Reference Values; D013194:Staining and Labeling; D014908:Triticum / Q000469:parasitology
chemical_list: D002241:Carbohydrates; D037102:Lectins