title: Evaluation of protective efficacy induced by virus-like particles containing a Trichinella spiralis excretory-secretory (ES) protein in mice.
abstract: The frequent outbreaks of human trichinellosis globally underscore the need to develop effective vaccine. We hypothesized that a novel vaccine could improve vaccine efficacy against Trichinella spiralis.
  
  
  
  In this study, we developed virus-like particles (VLPs) containing the 53 KDa excretory/secretory (ES) protein of T. spiralis and the influenza matrix protein 1 (M1) as a core protein, and investigated the protective efficacy of the VLPs alone or with cholera toxin (CT) in a mouse model.
  
  
  
  Intramuscular immunization induced T. spiralis-specific IgG, IgG1 and IgG2a antibody responses before and after challenge infections in the sera. These antibody responses were significantly enhanced in mice immunized with adjuvanted VLPs. Upon challenge infection, vaccinated mice showed significantly reduced worm burden in the diaphragm. Protective immune responses and efficacy of protection were significantly improved by immunization with VLPs together with CT adjuvant.
  
  
  
  Our results are informative for a better understanding of the protective immunity induced by T. spiralis VLPs, and will provide insight into designing safe and effective vaccines.
authors.affiliation: Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul, South Korea.|Department of Medical Zoology, Kyung Hee University School of Medicine, Seoul, South Korea.
authors.forename: Su-Hwa
authors.initials: SH
authors.lastname: Lee
authors.affiliation: Department of Medical Zoology, Kyung Hee University School of Medicine, Seoul, South Korea.
authors.forename: Sang-Soo
authors.initials: SS
authors.lastname: Kim
authors.affiliation: Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul, South Korea.|Department of Medical Zoology, Kyung Hee University School of Medicine, Seoul, South Korea.
authors.forename: Dong-Hun
authors.initials: DH
authors.lastname: Lee
authors.affiliation: Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul, South Korea.|Department of Medical Zoology, Kyung Hee University School of Medicine, Seoul, South Korea.
authors.forename: Ah-Ra
authors.initials: AR
authors.lastname: Kim
authors.affiliation: Department of Medical Zoology, Kyung Hee University School of Medicine, Seoul, South Korea. fsquan@khu.ac.kr.
authors.forename: Fu-Shi
authors.initials: FS
authors.lastname: Quan
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000276:immunology*; D002772:Cholera Toxin / Q000276:immunology; D004195:Disease Models, Animal; D005260:Female; D015801:Helminth Proteins / Q000276:immunology*; D006801:Humans; D007114:Immunization; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D017160:Trichinella spiralis / Q000276:immunology* / Q000302:isolation & purification; D014235:Trichinellosis / Q000517:prevention & control*; D058425:Vaccines, Virus-Like Particle / Q000276:immunology / Q000592:standards*
chemical_list: D000276:Adjuvants, Immunologic; D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015801:Helminth Proteins; D007074:Immunoglobulin G; D058425:Vaccines, Virus-Like Particle; C097419:excretory-secretory antigen, Trichinella; D002772:Cholera Toxin
keywords: Cholera toxin; Protection; Trichinella spiralis; Vaccine; Virus-like particle