title: Genetic characterization and transcription analyses of the European sea bass (Dicentrarchus labrax) isg15 gene.
abstract: Fish interferons are cytokines involved in its resistance to viral infections by inducing the transcription of several interferon-induced genes, such as isg15. The aim of the present study was the genetic characterization of the European sea bass isg15 gene, describing the regulatory motifs found in its sequence. In addition, an in vivo analysis of transcription in response to betanodavirus (RGNNV genotype) and poly I:C has been performed. The analysis of the resulting sequences showed that sea bass isg15 gene is composed of two exons and a single 276-bp intron located at the 5'-UTR region. The full length cDNA is 1143-bp, including a 102-bp 5'-UTR region, a 474-bp ORF, and a 291-bp 3'-UTR region. Several mRNA-regulatory elements, including three unusual ATTTA instability motifs in the intron, and four ATTTA motifs along with a cytoplasmic polyadenylation element in the 3'-UTR region, have been found in this sequence. The in vivo analyses revealed a similar kinetics and level of transcription in fish brain and head kidney after poly I:C inoculation; however, the induction caused by RGNNV started earlier in brain, where the upregulation of isg15 gene transcription was high. The present study contributes to further characterize the European sea bass IFN I response against RGNNV infections.
authors.affiliation: Universidad de Málaga, Departamento de Microbiología, Facultad de Ciencias, 29071 Málaga, Spain.
authors.forename: Patricia
authors.initials: P
authors.lastname: Moreno
authors.affiliation: Universidad de Málaga, Departamento de Microbiología, Facultad de Ciencias, 29071 Málaga, Spain.
authors.forename: Esther
authors.initials: E
authors.lastname: Garcia-Rosado
authors.affiliation: Universidad de Málaga, Departamento de Microbiología, Facultad de Ciencias, 29071 Málaga, Spain.
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Borrego
authors.affiliation: Universidad de Málaga, Departamento de Microbiología, Facultad de Ciencias, 29071 Málaga, Spain. Electronic address: mdalonso@uma.es.
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Alonso
mesh_terms: D000818:Animals; D001492:Bass; D003001:Cloning, Molecular; D016207:Cytokines / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D005393:Fish Diseases / Q000235:genetics* / Q000382:microbiology / Q000821:virology; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D030381:Nodaviridae / Q000502:physiology; D011070:Poly I-C / Q000494:pharmacology; D012327:RNA Virus Infections / Q000235:genetics / Q000382:microbiology / Q000662:veterinary* / Q000821:virology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA / Q000662:veterinary; D020539:Sequence Analysis, Protein / Q000662:veterinary; D014158:Transcription, Genetic; D014452:Ubiquitins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D016207:Cytokines; D018076:DNA, Complementary; D029941:Fish Proteins; D012333:RNA, Messenger; D014452:Ubiquitins; D011070:Poly I-C
keywords: Dicentrarchus labrax; European sea bass; Gene structure; Poly I:C; RGNNV; isg15