title: The galactoside 2-α-L-fucosyltransferase FUT1 from Arabidopsis thaliana: crystallization and experimental MAD phasing.
abstract: The plant cell wall is a complex network of polysaccharides made up of cellulose, hemicelluloses and pectins. Xyloglucan (XyG), which is the main hemicellulosic component of dicotyledonous plants, has attracted much attention for its role in plant development and for its many industrial applications. The XyG-specific fucosyltransferase (FUT1) adds a fucose residue from GDP-fucose to the 2-O position of the terminal galactosyl residues on XyG side chains. Recombinant FUT1 from Arabidopsis thaliana was crystallized in two different crystal forms, with the best diffracting crystals (up to 1.95 Å resolution) belonging to the monoclinic space group P21, with unit-cell parameters a = 87.6, b = 84.5, c = 150.3 Å, β = 96.3°. Ab initio phases were determined using a two-wavelength anomalous dispersion experiment on a tantalum bromide-derivatized crystal with data collected at the rising and descending inflection points of the Ta white line. An interpretable electron-density map was obtained after elaborate density modification. Model completion and structural analysis are currently under way.
authors.affiliation: Université Grenoble Alpes, Grenoble, France.
authors.forename: Joana
authors.initials: J
authors.lastname: Rocha
authors.affiliation: Université Grenoble Alpes, Grenoble, France.
authors.forename: Félix
authors.initials: F
authors.lastname: Cicéron
authors.affiliation: Université Grenoble Alpes, Grenoble, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Lerouxel
authors.affiliation: Université Grenoble Alpes, Grenoble, France.
authors.forename: Christelle
authors.initials: C
authors.lastname: Breton
authors.affiliation: ESRF - The European Synchrotron, 71 Avenue des Martyrs, 38000 Grenoble, France.
authors.forename: Daniele
authors.initials: D
authors.lastname: de Sanctis
mesh_terms: D000818:Animals; D017360:Arabidopsis / Q000737:chemistry* / Q000201:enzymology; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D016367:Baculoviridae / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray; D005647:Fucosyltransferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005936:Glucans / Q000737:chemistry* / Q000378:metabolism; D006154:Guanosine Diphosphate Fucose / Q000737:chemistry* / Q000378:metabolism; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D061987:Sf9 Cells; D018411:Spodoptera; D014961:X-Ray Diffraction; D014990:Xylans / Q000737:chemistry* / Q000378:metabolism; D000097631:Galactoside 2-alpha-L-fucosyltransferase
chemical_list: D029681:Arabidopsis Proteins; D005647:Fucosyltransferases; D005936:Glucans; D006154:Guanosine Diphosphate Fucose; D011994:Recombinant Proteins; D014990:Xylans; D000097631:Galactoside 2-alpha-L-fucosyltransferase; C029353:xyloglucan
keywords: Arabidopsis thaliana; MAD; fucosyltransferase; plant cell walls; tantalum bromide; xyloglucan