title: Genotoxic evaluation of Halfenprox using the human peripheral lymphocyte micronucleus assay and the Ames test.
abstract: The genotoxicity and mutagenicity of Halfenprox, a synthetic pyrethroid insecticide and acaricide, was assessed using two standard genotoxicity assays of the Salmonella typhimurium mutagenicity assay (Ames test) and in vitro micronucleus (MN) assay in human peripheral lymphocytes. In the Ames test, Salmonella strains TA98 and TA100 were treated with or without S9 fraction. The doses of Halfenprox were 6.25, 12.5, 25, 50, and 100 μg/plate and test materials were dissolved in DMSO. The concentrations of Halfenprox did not show mutagenic activity on both strains with and without S9 fraction. The MN assay was used to investigate the genotoxic effects of Halfenprox in human peripheral lymphocytes treated with 250, 500, 750, and 1000 μg/ml concentrations of Halfenprox for 24 and 48 h, and at 1000 μg/ml the concentration was significantly increased and the MN formation was compared with the negative control for both treatment periods. In addition, a significant decrease of the nuclear devision index (NDI) values at the higher concentrations of Halfenprox and at both treatment periods was observed.
authors.affiliation: a Department of Biology , Faculty of Arts and Sciences, Afyon Kocatepe University , Afyonkarahisar , Turkey.
authors.forename: Dilek
authors.initials: D
authors.lastname: Akyıl
authors.affiliation: b Department of Science , Faculty of Education, Suleyman Demirel University , Isparta , Turkey , and.
authors.forename: Yasin
authors.initials: Y
authors.lastname: Eren
authors.affiliation: c Department of Molecular Biology and Genetics (English) , Faculty of Engineering and Natural Sciences, Üsküdar University , Istanbul , Turkey.
authors.forename: Muhsin
authors.initials: M
authors.lastname: Konuk
authors.affiliation: a Department of Biology , Faculty of Arts and Sciences, Afyon Kocatepe University , Afyonkarahisar , Turkey.
authors.forename: Hatice
authors.initials: H
authors.lastname: Dere
authors.affiliation: a Department of Biology , Faculty of Arts and Sciences, Afyon Kocatepe University , Afyonkarahisar , Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Serteser
mesh_terms: D056810:Acaricides / Q000633:toxicity*; D004269:DNA, Bacterial / Q000187:drug effects* / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007306:Insecticides / Q000633:toxicity*; D048629:Micronuclei, Chromosome-Defective / Q000139:chemically induced*; D015162:Micronucleus Tests; D009154:Mutation; D010647:Phenyl Ethers / Q000633:toxicity*; D011722:Pyrethrins / Q000633:toxicity*; D018570:Risk Assessment; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics; D013997:Time Factors
chemical_list: D056810:Acaricides; D004269:DNA, Bacterial; D007306:Insecticides; D010647:Phenyl Ethers; D011722:Pyrethrins; C000616634:halfenprox
keywords: Ames test; Halfenprox; genotoxicity; micronucleus assay; mutagenicity; nuclear division index