title: In vitro investigation of nanohydroxyapatite/poly(L-lactic acid) spindle composites used for bone tissue engineering.
abstract: Calcium phosphate ceramics such as synthetic hydroxyapatite and tricalcium phosphate are widely used in the clinic, but they stimulate less bone regeneration. In this paper, nano-hydroxyapatite/poly(L-lactic acid) (nano-HA/PLLA) spindle composites with good mechanical performance were fabricated by a modified in situ precipitation method. The HA part of composite, distributing homogenously in PLLA matrix, is spindle shape with size of 10-30 nm in diameter and 60-100 nm in length. The molar ratio of Ca/P in the synthesized nano-HA spindles was deduced as 1.52 from the EDS spectra, which is close to the stoichiometric composition of HA (Ca/P & 1.67). The compress strength is up to 150 MPa when the HA content increase to 20 %. The in vitro tests indicate that HA/PLLA bio-composites have good biodegradability and bioactivity when immersed in simulated body fluid solutions. All the results suggested that HA/PLLA nano-biocomposites are appropriate to be applied as bone substitute in bone tissue engineering.
authors.affiliation: School of Nursing, Anhui San Lian University, Hefei, 230601, China.
authors.forename: W
authors.initials: W
authors.lastname: Yan
authors.affiliation: College of Pharmacy, Anhui University of Chinese Medicine, Hefei, China. cyzhang@163.com.|Key Laboratory of Materials Physics, Institute of Solid State Physics, Chinese Academy of Sciences, Hefei, 230031, China. cyzhang@163.com.
authors.forename: C Y
authors.initials: CY
authors.lastname: Zhang
authors.affiliation: Armor Forces Department, Army Officer Academy of PLA, Hefei, China.
authors.forename: L L
authors.initials: LL
authors.lastname: Xia
authors.affiliation: Key Laboratory of Materials Physics, Institute of Solid State Physics, Chinese Academy of Sciences, Hefei, 230031, China.
authors.forename: T
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Materials Physics, Institute of Solid State Physics, Chinese Academy of Sciences, Hefei, 230031, China.
authors.forename: Q F
authors.initials: QF
authors.lastname: Fang
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D001826:Body Fluids / Q000737:chemistry; D001861:Bone Regeneration; D018786:Bone Substitutes / Q000737:chemistry*; D001842:Bone and Bones; D017886:Durapatite / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology; D011091:Polyesters / Q000737:chemistry*; D013314:Stress, Mechanical; D013499:Surface Properties; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D011091:Polyesters; C033616:poly(lactide); D017886:Durapatite