title: Differential Matrix Metalloprotease (MMP) Expression Profiles Found in Aged Gingiva.
abstract: The periodontium undergoes age-related cellular and clinical changes, but the involved genes are not yet known. Here, we investigated age-related genetic changes in gingiva at the transcriptomic level. Genes that were differentially expressed between young and old human gingiva were identified by RNA sequencing and verified by real-time PCR. A total of 1939 mRNA transcripts showed significantly differential expression between young and old gingival tissues. Matrix metalloprotease (MMP) regulation was the top pathway involved in gingival aging. MMP3, MMP9, MMP12, and MMP13 were upregulated in old gingival tissues, concomitantly with interleukin-1 beta (IL1B) expression. In vitro experiments using human gingival fibroblasts (hGFs) showed that MMP12 was upregulated in old hGFs compared to young hGFs. Moreover, the MMP3, MMP9 and IL1B levels were more highly stimulated by infection with the oral bacterium, Fusobacterium nucleatum, in old hGFs compared to young hGFs. Collectively, these findings suggest that, in gingiva, the upregulation of MMP12 may be a molecular hallmark of natural aging, while the upregulations of MMP3, MMM9, and IL1B may indicate externally (e.g., infection)-induced aging. These findings contribute to our understanding of the molecular targets involved in gingival aging.
authors.affiliation: Department of Oral Biochemistry, Dental Science Research Institute, Medical Research Center for Biomineralization Disorders, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea.|Department of Molecular Medicine (BK21plus), Chonnam National University Graduate School, Gwangju, Republic of Korea.
authors.forename: Suhee
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Oral Biochemistry, Dental Science Research Institute, Medical Research Center for Biomineralization Disorders, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea.
authors.forename: Sun Hee
authors.initials: SH
authors.lastname: Ahn
authors.affiliation: Department of Oral Biochemistry, Dental Science Research Institute, Medical Research Center for Biomineralization Disorders, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea.
authors.forename: Jin-Sil
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Department of Molecular Medicine (BK21plus), Chonnam National University Graduate School, Gwangju, Republic of Korea.
authors.forename: Ji-Eun
authors.initials: JE
authors.lastname: Song
authors.affiliation: Department of Molecular Medicine (BK21plus), Chonnam National University Graduate School, Gwangju, Republic of Korea.
authors.forename: Sung-Hyun
authors.initials: SH
authors.lastname: Cho
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea.
authors.forename: Seunggon
authors.initials: S
authors.lastname: Jung
authors.affiliation: Personalized Genomic Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea.
authors.forename: Seon-Kyu
authors.initials: SK
authors.lastname: Kim
authors.affiliation: Aging Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea.
authors.forename: Seok-Ho
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Aging Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea.
authors.forename: Kwang-Pyo
authors.initials: KP
authors.lastname: Lee
authors.affiliation: Aging Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea.
authors.forename: Ki-Sun
authors.initials: KS
authors.lastname: Kwon
authors.affiliation: Department of Oral Biochemistry, Dental Science Research Institute, Medical Research Center for Biomineralization Disorders, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea.|Department of Molecular Medicine (BK21plus), Chonnam National University Graduate School, Gwangju, Republic of Korea.
authors.forename: Tae-Hoon
authors.initials: TH
authors.lastname: Lee
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000201:enzymology*; D005674:Fusobacterium Infections / Q000201:enzymology; D016967:Fusobacterium nucleatum; D018093:Gelatinases / Q000096:biosynthesis*; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D005881:Gingiva / Q000201:enzymology* / Q000382:microbiology; D005891:Gingivitis / Q000201:enzymology / Q000382:microbiology; D006801:Humans; D053583:Interleukin-1beta / Q000096:biosynthesis; D008297:Male; D008875:Middle Aged
chemical_list: C508585:IL1B protein, human; D053583:Interleukin-1beta; D018093:Gelatinases