title: Simultaneous quantitation and comparison of eight components in Jiao-ai decoction and Si-wu decoction by ultra high performance liquid chromatography with triple quadrupole tandem mass spectrometry.
abstract: An ultra high performance liquid chromatography coupled to triple quadrupole tandem mass spectrometry method has been established to evaluate the variations of multiple components of Chinese herbal preparations, Jiao-ai decoction and Si-wu decoction, through the simultaneous determination of eight major active compounds with a huge difference in the level of content. Chromatographic separation was achieved on a Waters Acquity UPLC BEH C18 column (2.1 × 100 mm, 1.7 μm) with a mobile phase consisting of acetonitrile (0.1% formic acid) and water (0.1% formic acid) under gradient elution. A triple quadrupole tandem mass spectrometer was operated in positive and negative ionization modes, respectively, with multiple reaction monitoring for the detection of the eight compounds. All calibration curves showed excellent linear regressions (r > 0.99) within the test range. The precision, repeatability, and stability of the eight compounds were below 5.0% in terms of relative standard deviation. The recoveries were 97.0-102.4% with a relative standard deviation of 1.21-3.65% for all samples. In conclusion, a rapid, sensitive, precise, accurate, and reliable method has been developed for the simultaneous detection of eight active compounds in the pharmaceutical samples of Jiao-ai decoction and Si-wu decoction, which can be applied for the multicomponent comparison and further quality control.
authors.affiliation: Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.|Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.|School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.
authors.forename: Weifeng
authors.initials: W
authors.lastname: Yao
authors.affiliation: Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.|Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.|School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.|Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.|School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.
authors.forename: Qinan
authors.initials: Q
authors.lastname: Liu
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Pathology, University of Washington, Seattle, WA, USA.
authors.forename: Jin
authors.initials: J
authors.lastname: Dai
authors.affiliation: Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.|Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.|School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.
authors.forename: Beihua
authors.initials: B
authors.lastname: Bao
authors.affiliation: Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China. zhangliguanxiong@163.com.|Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China. zhangliguanxiong@163.com.|School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China. zhangliguanxiong@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.|Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.|School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.
authors.forename: Anwei
authors.initials: A
authors.lastname: Ding
authors.affiliation: Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.|Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.
authors.forename: Yuping
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.|Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China.
authors.forename: Jinao
authors.initials: J
authors.lastname: Duan
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; C502769:si-wu decoction
keywords: Jiao-ai decoction; Multicomponent quantitation; Multiple reaction monitoring/ Si-wu decoction; Traditional Chinese medicine