title: N-Carbamoyl-L-Cysteine as an Intermediate in the Bioconversion from D,L-2-Amino-Δ (2)-Thiazoline-4-Carboxylic Acid to L-Cysteine by Pseudomonas sp. ON-4a.
abstract: We investigated the conversion of D,L-2-amino-Δ (2)-thiazoline-4-carboxylic acid (D,L-ATC) to L-cysteine with Pseudomonas sp. ON-4a, an ATC-assimilating bacterium. Cysteine and N-carbamoylcysteine (NCC), but not S-carbamoylcysteine (SCC), were produced from D,L-ATC by a cell-free extract from the strain. These products were isolated from the reaction mixture and then identified as the L-form. Similar results were obtained with P. putida AJ3865 and unidentified strain TG-3, an ATC-assimilating bacteria. It became clear that L-NCC is an intermediate in the conversion of D,L-ATC to L-cysteine in these Pseudomonas strains. Furthermore, it was suggested that these bacteria have L-ATC hydrolase and L-NCC amidohydrolase.
authors.affiliation: a Nippon Rikagakuyakuhin Co., Ltd., Research Center.
authors.forename: Y
authors.initials: Y
authors.lastname: Tamura
authors.affiliation: b Department of Science of Bioresource, Faculty of Agriculture, Hirosaki University.
authors.forename: M
authors.initials: M
authors.lastname: Nishino
authors.affiliation: b Department of Science of Bioresource, Faculty of Agriculture, Hirosaki University.
authors.forename: T
authors.initials: T
authors.lastname: Ohmachi
authors.affiliation: b Department of Science of Bioresource, Faculty of Agriculture, Hirosaki University.
authors.forename: Y
authors.initials: Y
authors.lastname: Asada
keywords: 2-amino-Δ 2-thiazoline-4-carboxylic acid (ATC); L-cysteine; N-carbamoyl-L-cysteine (L-NCC); Pseudomonas species; bioconversion