title: A method for easily customizable gradient gel electrophoresis.
abstract: Gradient polyacrylamide gel electrophoresis is a powerful tool for the resolution of polypeptides by relative mobility. Here, we present a simplified method for generating polyacrylamide gradient gels for routine analysis without the need for specialized mixing equipment. The method allows for easily customizable gradients which can be optimized for specific polypeptide resolution requirements. Moreover, the method eliminates the possibility of buffer cross contamination in mixing equipment, and the time and resources saved with this method in place of traditional gradient mixing, or the purchase of pre-cast gels, are noteworthy given the frequency with which many labs use gradient gel SDS-PAGE.
authors.affiliation: Department of Biological Sciences, DePaul University, 2325 N. Clifton Ave, Chicago, IL 60614, USA.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Miller
authors.affiliation: Department of Biological Sciences, DePaul University, 2325 N. Clifton Ave, Chicago, IL 60614, USA.
authors.forename: Brandon
authors.initials: B
authors.lastname: Roman
authors.affiliation: Department of Biological Sciences, DePaul University, 2325 N. Clifton Ave, Chicago, IL 60614, USA. Electronic address: enorstro@depaul.edu.
authors.forename: Eric
authors.initials: E
authors.lastname: Norstrom
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D057809:HEK293 Cells; D006801:Humans
keywords: Electrophoresis; Gel; Glycine; Gradient; Polyacrylamide; SDS-PAGE; Tricine