title: Increased Efficacy of Brentuximab Vedotin (SGN-35) in Combination with Cytokine-Induced Killer Cells in Lymphoma.
abstract: Brentuximab vedotin (SGN-35) is an antibody-drug conjugate with a high selectivity against CD30⁺ cell lines and more than 300-fold less activity against antigen-negative cells. In the last years, the results of many in vitro and in vivo studies have led to the fast approval of this drug to treat lymphoma patients. Another innovative method to treat tumor cells including lymphoma cells is the use cytokine-induced killer (CIK) cells, which have also been approved and proven to be a safe treatment with only minor adverse events. In this study, a possible additive effect when combining SGN-35 with CIK cells was investigated. The combinational treatment showed that it reduces the viability of CD30⁺ cell lines significantly in vitro. Additionally, the amount of lymphoma cells was significantly reduced when exposed to CIK cells as well as when exposed to SGN-35. A significant negative effect of SGN-35 on the function of CIK cells could be excluded. These results lead to the assumption that SGN-35 and CIK cells in combination might achieve better results in an in vitro setting compared to the single use of SGN-35 and CIK cells. Further investigations in in vivo models must be conducted to obtain a better understanding of the exact mechanisms of both treatments when applied in combination.
authors.affiliation: Center for Integrated Oncology (CIO), Medizinische Klinik und Poliklinik III, University of Bonn, Sigmund-Freud-Straße 25, 53105 Bonn, Germany. laura92esser@googlemail.com.
authors.forename: Laura
authors.initials: L
authors.lastname: Esser
authors.affiliation: Hochschule Bonn-Rhein-Sieg, 53359 Rheinbach, Germany. Hans.Weiher@h-brs.de.
authors.forename: Hans
authors.initials: H
authors.lastname: Weiher
authors.affiliation: Center for Integrated Oncology (CIO), Medizinische Klinik und Poliklinik III, University of Bonn, Sigmund-Freud-Straße 25, 53105 Bonn, Germany. ingo.schmidt-wolf@ukb.uni-bonn.de.
authors.forename: Ingo
authors.initials: I
authors.lastname: Schmidt-Wolf
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects*; D000079963:Brentuximab Vedotin; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D055612:Cytokine-Induced Killer Cells / Q000166:cytology / Q000276:immunology*; D006801:Humans; D018796:Immunoconjugates / Q000494:pharmacology* / Q000627:therapeutic use; D007371:Interferon-gamma / Q000494:pharmacology; D017730:Ki-1 Antigen / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects; D008223:Lymphoma / Q000188:drug therapy / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D018796:Immunoconjugates; D017730:Ki-1 Antigen; D000079963:Brentuximab Vedotin; D007371:Interferon-gamma
keywords: CD30+ cells; SGN-35; antibody–drug conjugate; cytokine-induced killer (CIK) cells; lymphoma