title: Molecular cloning, expression and the adjuvant effects of interleukin-8 of channel catfish (Ictalurus Punctatus) against Streptococcus iniae.
abstract: Interleukin-8 (IL-8) as an important cytokine involving in inflammatory and immune response, has been studied as effective adjuvants for vaccines in mammals. However, there are fewer reports about the characterization and adjuvant effects of IL-8 in fish. In this study, cloning and sequence analysis of IL-8 coding region of channel catfish (Ictalurus punctatus) were conducted, mature IL-8(rtIL-8) was expressed and evaluated for its adjuvant effects on the immunoprotection of subunit vaccine encoding α-enolase (rENO) of Streptococcus iniae from several aspects in channel catfish. The results showed co-vaccination of rENO with rtIL-8 enhanced immune responses including humoral and cellular immunity, with higher relative percent survival(RPS,71.4%) compared with the moderate RPS of rENO alone(50%) against S. iniae infection at 4 week post vaccination. While rtIL-8 failed to maintain long-lasting immune protection, only with RPS of 26.67% in rENO + rtIL-8-vaccinated fish compared with that of rENO alone(20%) at 8 week, signifying that IL-8 hold promise for use as potential immunopotentiator in vaccines against bacterial infections in fish, whereas it is insufficient to extend the immunoprotection for long time, and further studies are required to understand the mechanisms of IL-8 used as an adjuvant and seek for more effective way to strengthen the adjuvanticity of IL-8.
authors.affiliation: Department of Basic Veterinary, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.
authors.forename: Erlong
authors.initials: E
authors.lastname: Wang
authors.affiliation: Department of Basic Veterinary, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Basic Veterinary, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Long
authors.affiliation: Department of Basic Veterinary, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.|Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.
authors.forename: Kaiyu
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Basic Veterinary, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Basic Veterinary, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.
authors.forename: Defang
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Basic Veterinary, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.|Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Geng
authors.affiliation: Department of Aquaculture, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Huang
authors.affiliation: Department of Basic Veterinary, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Ouyang
authors.affiliation: Department of Basic Veterinary, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Lai
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000907:Antibodies, Bacterial / Q000378:metabolism; D001428:Bacterial Vaccines / Q000276:immunology*; D003001:Cloning, Molecular; D005393:Fish Diseases / Q000276:immunology*; D029941:Fish Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D007059:Ictaluridae / Q000276:immunology*; D016209:Interleukin-8 / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D010751:Phosphopyruvate Hydratase / Q000235:genetics; D013290:Streptococcal Infections / Q000276:immunology*; D000070058:Streptococcus iniae / Q000276:immunology*; D014611:Vaccination; D019444:Vaccines, DNA / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D029941:Fish Proteins; D016209:Interleukin-8; D019444:Vaccines, DNA; D010751:Phosphopyruvate Hydratase