title: Untargeted mass spectrometric approach in metabolic healthy offspring of patients with type 2 diabetes reveals medium-chain acylcarnitine as potential biomarker for lipid induced glucose intolerance (LGIT).
abstract: Offspring of type 2 diabetes (T2D) patients have increased risk to develop diabetes, due to inherited genetic susceptibility that directly interferes with the individual adaption to environmental conditions. We characterise T2D offspring (OSP) to identify metabolic risk markers for early disease prediction. Plasma of metabolically healthy OSP individuals (n = 43) was investigated after an oral lipid tolerance test (oLTT) by an untargeted mass spectrometric approach for holistic metabolome analyses. Two subgroups of OSP probands can be separated by oLTT, although not differing in general clinical parameters. Analyses of the plasma metabolome revealed mainly medium-chain acylcarnitines and very long-chain fatty acids with differential abundance in the subgroups. The study presented indicates that metabolically healthy OSP of T2D patients differ upon metabolic challenging in serum metabolite composition, especially medium-chain acylcarnitines. The difference suggest that postprandial lipid induced glucose intolerance (LGIT) may serve as a further valuable marker for early diabetes prediction.
authors.affiliation: a Leibniz Center for Diabetes Research, Institute of Clinical Biochemistry and Pathobiochemistry, German Diabetes Center at the Heinrich-Heine-University Duesseldorf , Düsseldorf , Germany.|b German Center for Diabetes Research (DZD), Muenchen-Neuherberg , Germany.
authors.forename: Birgit
authors.initials: B
authors.lastname: Knebel
authors.affiliation: c Institute for Diabetes Research, Department of General Internal Medicine, Asklepios Klinik St. Georg, Medical Faculty of Semmelweis University , Hamburg , Germany , and.
authors.forename: Susanne
authors.initials: S
authors.lastname: Mack
authors.affiliation: a Leibniz Center for Diabetes Research, Institute of Clinical Biochemistry and Pathobiochemistry, German Diabetes Center at the Heinrich-Heine-University Duesseldorf , Düsseldorf , Germany.|b German Center for Diabetes Research (DZD), Muenchen-Neuherberg , Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Lehr
authors.affiliation: d Bruker Daltonik GmbH , Bremen , Germany , and.
authors.forename: Aiko
authors.initials: A
authors.lastname: Barsch
authors.affiliation: a Leibniz Center for Diabetes Research, Institute of Clinical Biochemistry and Pathobiochemistry, German Diabetes Center at the Heinrich-Heine-University Duesseldorf , Düsseldorf , Germany.|b German Center for Diabetes Research (DZD), Muenchen-Neuherberg , Germany.
authors.forename: Martina
authors.initials: M
authors.lastname: Schiller
authors.affiliation: c Institute for Diabetes Research, Department of General Internal Medicine, Asklepios Klinik St. Georg, Medical Faculty of Semmelweis University , Hamburg , Germany , and.
authors.forename: Jutta
authors.initials: J
authors.lastname: Haas
authors.affiliation: c Institute for Diabetes Research, Department of General Internal Medicine, Asklepios Klinik St. Georg, Medical Faculty of Semmelweis University , Hamburg , Germany , and.
authors.forename: Simone
authors.initials: S
authors.lastname: Lange
authors.affiliation: d Bruker Daltonik GmbH , Bremen , Germany , and.
authors.forename: Jens
authors.initials: J
authors.lastname: Fuchser
authors.affiliation: d Bruker Daltonik GmbH , Bremen , Germany , and.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Zurek
authors.affiliation: e Department of Internal Medicine I , University Hospital Aachen , Aachen , Germany.
authors.forename: Dirk
authors.initials: D
authors.lastname: Müller-Wieland
authors.affiliation: a Leibniz Center for Diabetes Research, Institute of Clinical Biochemistry and Pathobiochemistry, German Diabetes Center at the Heinrich-Heine-University Duesseldorf , Düsseldorf , Germany.|b German Center for Diabetes Research (DZD), Muenchen-Neuherberg , Germany.
authors.forename: Jorg
authors.initials: J
authors.lastname: Kotzka
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000378:metabolism*; D001786:Blood Glucose / Q000378:metabolism*; D002331:Carnitine / Q000031:analogs & derivatives* / Q000378:metabolism; D016022:Case-Control Studies; D002853:Chromatography, Liquid; D003924:Diabetes Mellitus, Type 2 / Q000150:complications* / Q000503:physiopathology; D005260:Female; D018149:Glucose Intolerance / Q000175:diagnosis* / Q000209:etiology / Q000378:metabolism; D005951:Glucose Tolerance Test; D006801:Humans; D008055:Lipids / Q000009:adverse effects*; D008297:Male; D013058:Mass Spectrometry; D055442:Metabolome; D008875:Middle Aged; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D001786:Blood Glucose; D008055:Lipids; C116917:acylcarnitine; D002331:Carnitine
keywords: Healthy first degree T2D relatives; metabolic challenging; untargeted metabolome analyses