title: A Bioorthogonal Approach for the Preparation of a Titanium-Binding Insulin-like Growth-Factor-1 Derivative by Using Tyrosinase.
abstract: The generation of metal surfaces with biological properties, such as cell-growth-enhancing and differentiation-inducing abilities, could be potentially exciting for the development of functional materials for use in humans, including artificial dental implants and joint replacements. However, currently the immobilization of proteins on the surfaces of the metals are limited. In this study, we have used a mussel-inspired bioorthogonal approach to design a 3,4-hydroxyphenalyalanine-containing recombinant insulin-like growth-factor-1 using a combination of recombinant DNA technology and tyrosinase treatment for the surface modification of titanium. The modified growth factor prepared in this study exhibited strong binding affinity to titanium, and significantly enhanced the growth of NIH3T3 cells on the surface of titanium.
authors.affiliation: Nano Medical Engineering Laboratory, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama, 351-0198, Japan.|School of Pharmaceutical Sciences, Jilin University, No. 1266 Fujin Road, Changchun, Jilin, 130021, P.R. China.|Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Chinese Academy of Sciences, Changchun, Jilin, 130022, P.R. China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Nano Medical Engineering Laboratory, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama, 351-0198, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Miyatake
authors.affiliation: Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Chinese Academy of Sciences, Changchun, Jilin, 130022, P.R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Lipid Biology Laboratory, RIKEN, Japan.
authors.forename: Takehiko
authors.initials: T
authors.lastname: Inaba
authors.affiliation: School of Pharmaceutical Sciences, Jilin University, No. 1266 Fujin Road, Changchun, Jilin, 130021, P.R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Chinese Academy of Sciences, Changchun, Jilin, 130022, P.R. China.
authors.forename: Peibiao
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Nano Medical Engineering Laboratory, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama, 351-0198, Japan. y-ito@riken.jp.|Emergent Bioengineering Materials Research Team, RIKEN Center for Emergent Matter Science, 2-1 Hirosawa, Wako-shi, Saitama, 351-0198, Japan. y-ito@riken.jp.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ito
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D055767:Immobilized Proteins / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D051379:Mice; D014442:Monophenol Monooxygenase / Q000378:metabolism*; D041681:NIH 3T3 Cells; D058525:Quartz Crystal Microbalance Techniques; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D013499:Surface Properties; D053719:Tandem Mass Spectrometry; D014025:Titanium / Q000737:chemistry* / Q000378:metabolism
chemical_list: D055767:Immobilized Proteins; D011994:Recombinant Proteins; D007334:Insulin-Like Growth Factor I; D014025:Titanium; D014442:Monophenol Monooxygenase
keywords: bioorthogonal chemistry; cell growth; growth factors; titanium; tyrosinase