title: Interleukin-13 Inhibits Expression of cyp27b1 in Peripheral CD14+ Cells That Is Correlated With Vertebral Bone Mineral Density of Patients With Ulcerative Colitis.
abstract: Osteoporosis is a common problem in aged people and those with related diseases, such as inflammatory bowel diseases. Deregulation of vitamin D metabolism plays a role in the pathogenesis of osteoporosis. Micro RNA (miR) can regulate cytokine expression in cells. This study test a hypothesis that inflammatory cytokine interleukin (IL)-13 increases miR-19a to compromise cyp27b1 (a vitamin D hydroxylase) in peripheral CD14+cells. Bone mineral density of L2-L4 was measured in 20 patients with ulcerative colitis (UC) and 20 healthy subjects. Peripheral CD14+cells were isolated from healthy people and patients with UC. Expression of cyp27b1 by CD14+cells was analyzed in the presence or absence of IL-13 in the culture. We observed that bone mineral density (BMD) in UC patients was significantly lower than healthy subjects. The BMD is negatively correlated with miR-19a in peripheral CD14+cells. MiR-19a in peripheral CD14+cell was correlated with serum IL-13 in UC patients. Expression of cyp27b1 in peripheral CD14+cells was correlated with miR-19a and serum IL-13 in UC patients. IL-13 suppressed cyp27b1 expression in CD14+cells. IL-13 increased expression of miR-19a in CD14+cells. IL-13 suppresses cyp27b1 expression in peripheral CD14+cells via up regulating miR-19a expression. J. Cell. Biochem. 118: 376-381, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Orthopaedic Department, The First Affiliated Hospital of Nanjing Medical University, Jiangsu, China.
authors.forename: Qingfeng
authors.initials: Q
authors.lastname: Ding
authors.affiliation: Emergency Department, The First Affiliated Hospital of Nanjing Medical University, Jiangsu, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Orthopaedic Department, The First Affiliated Hospital of Nanjing Medical University, Jiangsu, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yun
authors.affiliation: Orthopaedic Department, The First Affiliated Hospital of Nanjing Medical University, Jiangsu, China.
authors.forename: Lingjie
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Orthopaedic Department, The First Affiliated Hospital of Nanjing Medical University, Jiangsu, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Orthopaedic Department, The First Affiliated Hospital of Nanjing Medical University, Jiangsu, China.
authors.forename: Guoyong
authors.initials: G
authors.lastname: Yin
authors.affiliation: Orthopaedic Department, The First Affiliated Hospital of Nanjing Medical University, Jiangsu, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Fan
mesh_terms: D015090:25-Hydroxyvitamin D3 1-alpha-Hydroxylase / Q000096:biosynthesis*; D000328:Adult; D015519:Bone Density; D003093:Colitis, Ulcerative / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D006801:Humans; D018793:Interleukin-13 / Q000494:pharmacology*; D007962:Leukocytes / Q000378:metabolism* / Q000473:pathology; D018950:Lipopolysaccharide Receptors; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis; D008875:Middle Aged; D010024:Osteoporosis / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D013131:Spine / Q000378:metabolism* / Q000473:pathology
chemical_list: D018793:Interleukin-13; D018950:Lipopolysaccharide Receptors; C549214:MIRN19A microRNA, human; D035683:MicroRNAs; D015090:25-Hydroxyvitamin D3 1-alpha-Hydroxylase; C000600378:CYP27B1 protein, human
keywords: CD14 CELL; INTERLEUKIN 13; MICRO RNA; OSTEOPOROSIS; ULCERATIVE COLITIS