title: Impact of calcium ion on cytotoxic effect of the boroxine derivative, K2[B3O3F4OH].
abstract: The effect of Ca2+ions on the cytotoxic ability of boron heterocyclic compound dipotassium-trioxohydroxytetrafluorotriborate (K2[B3O3F4OH]), on in vitro tumor cells (mammary adenocarcinoma 4T1, melanoma B16F10 and squamous cell carcinoma SCCVII) and non-tumoral fibroblast cells (mouse dermal L929 and hamster lung V79) was examined. At small concentrations of Ca2+ions (0.42 mM), K2[B3O3F4OH] (3.85 mM) has a very strong cytotoxic effect on all cancer cells tested (89.1, 85.6 and 84.6%) and significantly less effect on normal cells (19.5 and 24.2%), respectively. Applying larger concentrations of Ca2+ions (9.42-72.42 mM), at the same concentration of K2[B3O3F4OH], no significant cytotoxic effect was detected on cancer cells and normal cells investigated. The selective ability of K2[B3O3F4OH], in the medium with a low concentration of Ca2+ions has a strong cytotoxic effect on cancer cells and very weak effect in normal cells, opens up the possibility of its application in antitumor therapy.
authors.affiliation: a Division of Molecular Medicine , Rudjer Boskovic Institute , Zagreb , Croatia.
authors.forename: Sinisa
authors.initials: S
authors.lastname: Ivankovic
authors.affiliation: a Division of Molecular Medicine , Rudjer Boskovic Institute , Zagreb , Croatia.
authors.forename: Ranko
authors.initials: R
authors.lastname: Stojkovic
authors.affiliation: b Faculty of Science , University of Sarajevo , Sarajevo , Bosnia and Herzegovina , and.
authors.forename: Milka
authors.initials: M
authors.lastname: Maksimovic
authors.affiliation: b Faculty of Science , University of Sarajevo , Sarajevo , Bosnia and Herzegovina , and.
authors.forename: Borivoj
authors.initials: B
authors.lastname: Galic
authors.affiliation: c Faculty of Chemistry and Technology , University of Split , Split , Croatia.
authors.forename: Mladen
authors.initials: M
authors.lastname: Milos
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D001896:Boron Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D002118:Calcium / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D007477:Ions / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D015394:Molecular Structure; D013329:Structure-Activity Relationship
chemical_list: D000970:Antineoplastic Agents; D001896:Boron Compounds; D007477:Ions; C561431:dipotassium trioxohydroxytetrafluorotriborate; D002118:Calcium
keywords: Anticancer activity; boroxine; calcium ion; drug research