title: Toxicological Effects Induced by Silver Nanoparticles in Zebra Fish (Danio Rerio) and in the Bacteria Communities Living at Their Surface.
abstract: The antimicrobial activity of silver nanoparticles (AgNP) makes them useful in a wide range of products although their environmental impact is still uncertain. The main goal of this study was to evaluate short-term effects induced by AgNP on gills oxidative status and bacterial communities living at the skin mucus of zebrafish. Both the number of bacteria colony forming units and bacteria growth obtained from skin mucus were lower in all concentrations tested (25, 50 and 100 µg nAg/L). Besides, AgNP exposure caused a significant decrease in bacteria growth in zebrafish exposed to 100 µg nAg/L. AgNP accumulated in zebrafish gills at both highest concentrations tested, but this accumulation did not appear to result in oxidative stress. Overall the results indicated toxicological effects of AgNP on bacteria communities living at the zebrafish mucus surface. Although silver accumulation was verified in gills, no evidence of toxicity in terms of oxidative stress was found.
authors.affiliation: Instituto Nacional de Limnología (INALI-CONICET-UNL), Paraje El Pozo, Ciudad Universitaria UNL, 3000, Santa Fe, Argentina. carlabacchetta@yahoo.com.ar.
authors.forename: Carla
authors.initials: C
authors.lastname: Bacchetta
authors.affiliation: Nanotek S.A., Santa Fe, Argentina.|Universidad Tecnológica Nacional, Santa Fe, Argentina.
authors.forename: Gerardo
authors.initials: G
authors.lastname: López
authors.affiliation: Nanotek S.A., Santa Fe, Argentina.|Universidad Tecnológica Nacional, Santa Fe, Argentina.
authors.forename: Gisela
authors.initials: G
authors.lastname: Pagano
authors.affiliation: Departamento de Química, Universidade Federal de Santa María (UFSM), Santa María, RS, Brazil.
authors.forename: Diana Tomazi
authors.initials: DT
authors.lastname: Muratt
authors.affiliation: Departamento de Química, Universidade Federal de Santa María (UFSM), Santa María, RS, Brazil.
authors.forename: Leandro Machado
authors.initials: LM
authors.lastname: de Carvalho
authors.affiliation: Instituto de Ciências Biológicas (ICB), Universidade Federal do Rio Grande - FURG, Rio Grande, Brazil.|Programa de Pós-Graduação Em Ciências Fisiológicas, Universidade Federal de Rio Grande - FURG, Rio Grande, Brazil.|Programa de Pós-graduação em Aquicultura, Universidade Federal de Rio Grande - FURG, Rio Grande, Brazil.
authors.forename: José María
authors.initials: JM
authors.lastname: Monserrat
mesh_terms: D000818:Animals; D001419:Bacteria / Q000187:drug effects; D005880:Gills / Q000378:metabolism; D053768:Metal Nanoparticles / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D012834:Silver / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015027:Zebrafish
chemical_list: D014874:Water Pollutants, Chemical; D012834:Silver
keywords: Antibacterial activity; Bioaccumulation; Mucus; Nanomaterials; Nanosilver; Oxidative stress