title: PTCH-1 and MDM2 expression in ameloblastoma from a West African sub-population: implication for chemotherapeutics.
abstract: BACKGROUND
  Ameloblastoma is a slow growing, painless odontogenic swelling which can attain sizes that result in severe deformities of the craniofacial complex. It is the most commonly encountered odontogenic tumor in Nigeria. Surgical intervention is currently the method of treatment; however identification of altered molecular pathways may inform chemotherapeutic potential. The Protein Patched homolog 1 (PTCH-1) is overexpressed in ameloblastoma. Also, mutation in the MDM2 gene can reduce the tumor suppressor function of p53 and promote ameloblastoma growth. No study however has characterized the molecular profile of African cases of ameloblastoma with a view to developing chemotherapeutic alternatives. The objective was to characterize the PTCH-1 genetic profile of Ameloblastoma in Nigerian patients as a first step in investigating its potential for chemotherapeutic intervention.
  
  
  METHODS
  Twenty-eight FFPE blocks of ameloblastoma cases from Nigerian patients were prepared for antibody processing to PTCH-1 (Polyclonal Anti-PTCH antibody ab39266) and MDM2 (Monoclonal Anti-MDM2 antibody (2A10) ab16895). Cytoplasmic brown staining was considered as positive for PTCH while nuclear staining was positive for MDM2.
  
  
  RESULTS
  Moderate and strong expressions for PTCH in ameloblast and stellate reticulum were 78.6% and 60.7% respectively. Only 3 (10.7%) cases expressed MDM2.
  
  
  CONCLUSIONS
  The importance of our study is that it supports, in theory, anti-PTCH/SHH chemotherapeutics for Nigerian ameloblastoma cases and also infers the possible additional use of anti-p53 agents.
authors.affiliation: Department of Oral and Maxillofacial Surgery, College of Health Sciences, University of Port Harcourt, Nigeria.
authors.forename: Samuel Ebele
authors.initials: SE
authors.lastname: Udeabor
authors.affiliation: Department of Oral Pathology, College of Medicine, University of Ibadan, Nigeria.
authors.forename: Akinyele Olumuyiwa
authors.initials: AO
authors.lastname: Adisa
authors.affiliation: Department of Oral Pathology, College of Medicine, University of Ibadan, Nigeria.
authors.forename: Ahmed Oluwatoyin
authors.initials: AO
authors.lastname: Lawal
authors.affiliation: Department for Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University Frankfurt, Frankfurt am Main, Germany; REPAIR-Lab, Institute of Pathology, University Medical Center, Johannes Gutenberg University, Mainz, Germany.
authors.forename: Mike
authors.initials: M
authors.lastname: Barbeck
authors.affiliation: Department for Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University Frankfurt, Frankfurt am Main, Germany; REPAIR-Lab, Institute of Pathology, University Medical Center, Johannes Gutenberg University, Mainz, Germany.
authors.forename: Patrick
authors.initials: P
authors.lastname: Booms
authors.affiliation: Department for Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University Frankfurt, Frankfurt am Main, Germany.
authors.forename: Robert Alexander
authors.initials: RA
authors.lastname: Sader
authors.affiliation: Department for Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University Frankfurt, Frankfurt am Main, Germany; REPAIR-Lab, Institute of Pathology, University Medical Center, Johannes Gutenberg University, Mainz, Germany.
authors.forename: Shahram
authors.initials: S
authors.lastname: Ghanaati
mesh_terms: D000328:Adult; D000564:Ameloblastoma / Q000235:genetics* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007573:Jaw Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D009154:Mutation; D009549:Nigeria; D000072081:Patched-1 Receptor / Q000235:genetics*; D051736:Proto-Oncogene Proteins c-mdm2 / Q000235:genetics*
chemical_list: C000606236:PTCH1 protein, human; D000072081:Patched-1 Receptor; C497206:MDM2 protein, human; D051736:Proto-Oncogene Proteins c-mdm2
keywords: MdM2; PTCH-1; ameloblastoma; chemotherapeutics