title: Inflammation in low back pain may be detected from the peripheral blood: suggestions for biomarker.
abstract: Biomarker for prediction of development of low back pain, and disease progression in chronic conditions are virtually non-existent. In the present study, we examined evidence of inflammation in the peripheral blood and demonstrated significant changes in neuroinflammation markers in subjects with chronic low back pain in comparison with control subjects. The present study was performed using peripheral blood from subjects with chronic low back pain and age-matched control subjects. Western blotting, real-time RT-PCR, cell culture and in vitro assays were incorporated to perform the current study. We obtained evidence that the balance between proinflammatory and anti-inflammatory cytokines is misaligned, with decrease in interleukin-10 (IL-10) expression and increase in interleukin-6 (IL-6) expression. Furthermore, we demonstrated increase in CD16 monocyte expression. Cells were cultured under differential conditions to generate M1/M2 macrophages. In the macrophages, opioid secretory capacity was shown to be diminished. Finally, Dragon (repulsive guidance molecule b, RGMb) expression was shown diminished in M1 macrophages, which serves as a key transcriptional inhibitor of IL-6 expression. These biochemical and cellular alterations in chronic low back pain can serve as potential biomarkers for assessing disease initiation, intensity and progression.
authors.affiliation: Department of Orthopedics, Shaanxi Provincial People's Hospital, Xi'an, Shaanxi 710068, China yongli_med@sina.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Orthopedics, Shaanxi Provincial People's Hospital, Xi'an, Shaanxi 710068, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, Shaanxi Provincial People's Hospital, Xi'an, Shaanxi 710068, China.
authors.forename: Zong-Zhi
authors.initials: ZZ
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, Shaanxi Provincial People's Hospital, Xi'an, Shaanxi 710068, China.
authors.forename: Da-Peng
authors.initials: DP
authors.lastname: Duan
mesh_terms: D000368:Aged; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D015816:Cell Adhesion Molecules, Neuronal / Q000097:blood* / Q000276:immunology; D002454:Cell Differentiation; D002908:Chronic Disease; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000097:blood* / Q000276:immunology; D015850:Interleukin-6 / Q000097:blood* / Q000276:immunology; D017116:Low Back Pain / Q000097:blood* / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D008264:Macrophages / Q000276:immunology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology / Q000473:pathology; D061251:Primary Cell Culture; D058408:Th1-Th2 Balance; D014158:Transcription, Genetic; D001615:beta-Endorphin / Q000276:immunology / Q000378:metabolism
chemical_list: D015415:Biomarkers; D015816:Cell Adhesion Molecules, Neuronal; C508609:IL10 protein, human; C508600:IL6 protein, human; D015850:Interleukin-6; C560433:RGMB protein, human; D016753:Interleukin-10; D001615:beta-Endorphin
keywords: biomarker; inflammation; low back pain; molecular mechanism; transcription