title: Polyamines, myosin heavy chains, and collagen specific amino acids after a repeated bout of eccentric exercise.
abstract: We investigated alternatives to commonly used biomarkers of exercise-induced tissue damage. Over 5 days following two bouts of 100 drop-to-vertical jumps (inter-bout rest period of 3 weeks), myosin heavy chain 1, hydroxylysine (HYL), hydroxyproline (HYP), spermine (SPM) and spermine synthase (SMS) were measured in the serum of 10 participants. HYL significantly increased from 5.92 ± 1.49 ng/mL to 6.48 ± 1.47 ng/mL at 24 h. A similar trend was observed for bout 2, but without reaching significance. SPM significantly increased only after bout 1 from 0.96 ± 0.19 ng/mL at pretest to a peak level of 1.12 ± 0.26 ng/mL at 24 h, while B2 increments remained non-significant. Myosin heavy chain 1, HYP and SMS values remained below the detection limit of the applied enzyme-linked immunosorbent assay (ELISA) kit. Though HYL and SM increased after the intervention, both markers showed a large standard deviation (SD) combined with small increments. Therefore, none of the investigated biomarkers provides a meaningful alternative to commonly used damage markers.
authors.affiliation: a Institute of Training Science and Sport Informatics , German Sport University Cologne , Cologne , Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Behringer
authors.affiliation: a Institute of Training Science and Sport Informatics , German Sport University Cologne , Cologne , Germany.
authors.forename: Johannes Caspar Konrad
authors.initials: JC
authors.lastname: Montag
authors.affiliation: a Institute of Training Science and Sport Informatics , German Sport University Cologne , Cologne , Germany.
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Kilian
authors.affiliation: b Department of Mechanical and Design Engineering , University of Portsmouth , Portsmouth , UK.
authors.forename: Phil
authors.initials: P
authors.lastname: Heaton
authors.affiliation: a Institute of Training Science and Sport Informatics , German Sport University Cologne , Cologne , Germany.
authors.forename: Joachim
authors.initials: J
authors.lastname: Mester
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D015444:Exercise / Q000502:physiology*; D006801:Humans; D006901:Hydroxylysine / Q000097:blood; D006909:Hydroxyproline / Q000097:blood; D007958:Leukocyte Count; D008297:Male; D018482:Muscle, Skeletal / Q000473:pathology*; D063806:Myalgia / Q000097:blood / Q000209:etiology; D018995:Myosin Heavy Chains / Q000097:blood; D009504:Neutrophils; D013096:Spermine / Q000097:blood; D013097:Spermine Synthase / Q000097:blood; D055815:Young Adult
chemical_list: D015415:Biomarkers; D013096:Spermine; D006901:Hydroxylysine; D013097:Spermine Synthase; D018995:Myosin Heavy Chains; D006909:Hydroxyproline
keywords: Polyamines; hydroxylysine; hydroxyproline; myosin heavy chains; spermine; spermine synthase