title: Autofluorescent gelatin nanoparticles as imaging probes to monitor matrix metalloproteinase metabolism of cancer cells.
abstract: In this paper, autofluorescent gelatin nanoparticles were synthesized as matrix metalloproteinase (MMP) responsive probes for cancer cell imaging. A modified two-step desolvation method was employed to generate these nanoparticles whose size was controllable and had stable autofluorescence. As glutaraldehyde was introduced as the crosslinking agent, the generation of Schiff base (CN) and double carbon bond (CC) between glutaraldehyde and gelatin endowed these gelatin nanoparticles distinct autofluorescence. Considering MMPs were usually overexpressed on the surface of cancer cells and they had degradation ability toward gelatin, we utilized these nanoparticles as imaging probes to responsively monitor the MMP metabolism of cancer cells according to the luminance change. As fluorescent probes, these nanoparticles had facile synthesis procedure and good biocompatibility, and provided a smart strategy to monitor cancer cell behaviors. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2854-2860, 2016.
authors.affiliation: Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, School of Physics and Technology, Wuhan University, Wuhan, 430072, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Cai
authors.affiliation: Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, School of Physics and Technology, Wuhan University, Wuhan, 430072, China.
authors.forename: Lang
authors.initials: L
authors.lastname: Rao
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine of Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, China.
authors.forename: Xinghu
authors.initials: X
authors.lastname: Ji
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology and Key Laboratory of Oral Biomedicine, Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, 430072, China.
authors.forename: Lin-Lin
authors.initials: LL
authors.lastname: Bu
authors.affiliation: Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, School of Physics and Technology, Wuhan University, Wuhan, 430072, China.
authors.forename: Zhaobo
authors.initials: Z
authors.lastname: He
authors.affiliation: Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, School of Physics and Technology, Wuhan University, Wuhan, 430072, China.
authors.forename: Da
authors.initials: D
authors.lastname: Wan
authors.affiliation: Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, School of Physics and Technology, Wuhan University, Wuhan, 430072, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, School of Physics and Technology, Wuhan University, Wuhan, 430072, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, School of Physics and Technology, Wuhan University, Wuhan, 430072, China.
authors.forename: Shishang
authors.initials: S
authors.lastname: Guo
authors.affiliation: Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, School of Physics and Technology, Wuhan University, Wuhan, 430072, China. xzzhao@whu.edu.cn.
authors.forename: Xing-Zhong
authors.initials: XZ
authors.lastname: Zhao
mesh_terms: D002460:Cell Line; D045744:Cell Line, Tumor; D005453:Fluorescence; D005780:Gelatin / Q000737:chemistry*; D005976:Glutaral / Q000737:chemistry; D006801:Humans; D020782:Matrix Metalloproteinases / Q000032:analysis* / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry*; D009369:Neoplasms / Q000000981:diagnostic imaging / Q000201:enzymology* / Q000378:metabolism; D061848:Optical Imaging / Q000379:methods*
chemical_list: D005780:Gelatin; D020782:Matrix Metalloproteinases; D005976:Glutaral
keywords: autofluorescence; cell imaging; gelatin; matrix metalloproteinase; nanoparticles