title: [Expression of miR-22 and miR-150 in type 1 diabetes mellitus: Possible relationship with autoimmunity and clinical characteristics].
abstract: OBJECTIVE
  Type 1 diabetes (T1D) is an autoimmune disease of complex aetiology. Several microRNAs (miR) have been linked to the pathogenesis of autoimmune diseases. To analyze the possible association of miR-22 and miR-150 with autoimmunity and clinical severity of T1D.
  
  
  METHODS
  The study was performed in peripheral blood mononuclear cells of 20 patients with T1D and 20 control subjects. The expression of miR-22 and miR-150 was performed in peripheral blood mononuclear cells using TaqMan probes to different glucose concentrations (baseline, 11mm, 25mm).
  
  
  RESULTS
  Our results suggest that the expression of miR-22 is increased in T1D patients compared to the controls. This effect was observed in baseline glucose conditions and decreased in 11 and 25mM of glucose. The expression of miR-150 was lower in T1D patients versus the controls. There was no correlation between the autoimmune profile and the two studied miRNAs. miR-22 (baseline condition) and miR-150 (11mM condition) or the ketoacidosis component.
  
  
  CONCLUSIONS
  miR-22 and 150 were not associated with the autoimmune component present in T1D patients.
authors.affiliation: Laboratorio de Nutrigenómica, Departamento de Nutrición, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
authors.forename: Santiago
authors.initials: S
authors.lastname: Estrella
authors.affiliation: Laboratorio de Nutrigenómica, Departamento de Nutrición, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
authors.forename: Diego F
authors.initials: DF
authors.lastname: Garcia-Diaz
authors.affiliation: Instituto de Investigaciones Materno Infantil (IDIMI), Hospital San Borja Arriarán, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
authors.forename: Ethel
authors.initials: E
authors.lastname: Codner
authors.affiliation: Laboratorio de Nutrigenómica, Departamento de Nutrición, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
authors.forename: Patricia
authors.initials: P
authors.lastname: Camacho-Guillén
authors.affiliation: Laboratorio de Nutrigenómica, Departamento de Nutrición, Facultad de Medicina, Universidad de Chile, Santiago, Chile. Electronic address: fperez@med.uchile.cl.
authors.forename: Francisco
authors.initials: F
authors.lastname: Pérez-Bravo
mesh_terms: D000293:Adolescent; D000328:Adult; D015551:Autoimmunity / Q000235:genetics*; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000175:diagnosis / Q000235:genetics*; D005260:Female; D005819:Genetic Markers; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D055815:Young Adult
chemical_list: D015415:Biomarkers; D005819:Genetic Markers; D035683:MicroRNAs; C533097:MIR150, human; C548375:MIRN22 microRNA, human
keywords: Autoimmunity; Autoinmunidad; Diabetes tipo 1; Type 1 diabetes; miR-150; miR-22