title: Effects of dietary oxidized konjac glucomannan sulfates (OKGMS) and acidolysis-oxidized konjac glucomannan (A-OKGM) on the immunity and expression of immune-related genes of Schizothorax prenanti.
abstract: In the present study, konjac glucomannan (KGM) was degraded by H2O2, and then used trisulfonated sodium amine and HCl, individually, to obtain two kinds of derivatives: oxidized konjac glucomannan sulfates (OKGMS) and acidolysis-oxidized konjac glucomannan (A-OKGM). The effects of two OKGM modified products on the immune parameters and expressions of toll-like receptor 22 (TLR22), myeloid differentiation factor 88 (MyD88) and interferon regulatory factors 7 (IRF7) genes in Schizothorax prenanti were determined. The alternative haemolytic complement (ACH50) activity was found to be significantly increased by the OKGMS diets. The immunoglobulin M (IgM) level was significantly enhanced by the OKGMS diets. The lysozyme activity was significantly increased by both OKGMS and A-OKGM diets. The superoxide dismutase (T-SOD) activity in fish fed with all doses of OKGMS diets was significantly higher than that in fish fed with basal diet. The glutathione peroxidase (GSH-PX) activity in fish fed with 0.8% and 1.6% A-OKGM diets was significantly higher than control group. The malondialdehyde (MDA) level was significantly decreased by both OKGMS and A-OKGM diets. The 0.8% A-OKGM diet significantly up-regulated TLR22 gene expression in the head kidney and spleen. TLR22 gene expression was significantly promoted by all OKGMS diets in the mesonephros and liver. The MyD88 mRNA level in 1.6% A-OKGM group significantly increased in the head kidney. The low dose of OKGMS significantly induced the MyD88 gene expression in the mesonephros, gut and liver, while 0.8% A-OKGM group also showed a significantly enhanced MyD88 mRNA expression in the gut. High dose of OKGMS significantly increased the IRF7 mRNA expression in the mesonephros and spleen. Fish fed with low dose of A-OKGM showed significantly higher expression of IRF7 in the gut and liver. Present study suggested that OKGMS and A-OKGM can act as immunostimulant to improve the immune indexes and up-regulate the immune-related gene expressions.
authors.affiliation: College of Food Science, Sichuan Agricultural University, Yaan, 625014, Sichuan, PR China.
authors.forename: Mingrui
authors.initials: M
authors.lastname: Chen
authors.affiliation: College of Food Science, Sichuan Agricultural University, Yaan, 625014, Sichuan, PR China.
authors.forename: Hongjie
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Food Science, Sichuan Agricultural University, Yaan, 625014, Sichuan, PR China.
authors.forename: Qiuping
authors.initials: Q
authors.lastname: Yan
authors.affiliation: College of Food Science, Sichuan Agricultural University, Yaan, 625014, Sichuan, PR China.
authors.forename: Qiaoran
authors.initials: Q
authors.lastname: Zheng
authors.affiliation: College of Food Science, Sichuan Agricultural University, Yaan, 625014, Sichuan, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Yang
authors.affiliation: College of Food Science, Sichuan Agricultural University, Yaan, 625014, Sichuan, PR China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Lv
authors.affiliation: College of Food Science, Sichuan Agricultural University, Yaan, 625014, Sichuan, PR China.
authors.forename: Mei
authors.initials: M
authors.lastname: He
authors.affiliation: College of Food Science, Sichuan Agricultural University, Yaan, 625014, Sichuan, PR China.
authors.forename: Limei
authors.initials: L
authors.lastname: Feng
authors.affiliation: College of Food Science, Sichuan Agricultural University, Yaan, 625014, Sichuan, PR China.
authors.forename: Jiaqi
authors.initials: J
authors.lastname: Zhao
authors.affiliation: College of Food Science, Sichuan Agricultural University, Yaan, 625014, Sichuan, PR China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Tang
authors.affiliation: College of Food Science, Sichuan Agricultural University, Yaan, 625014, Sichuan, PR China. Electronic address: 405135288@qq.com.
authors.forename: Yinglong
authors.initials: Y
authors.lastname: Wu
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D003530:Cyprinidae / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D004032:Diet / Q000662:veterinary*; D019587:Dietary Supplements / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000276:immunology*; D007113:Immunity, Innate / Q000276:immunology*; D050839:Interferon Regulatory Factor-7 / Q000235:genetics / Q000378:metabolism; D008351:Mannans; D053594:Myeloid Differentiation Factor 88 / Q000235:genetics / Q000378:metabolism; D010084:Oxidation-Reduction; D011897:Random Allocation; D013431:Sulfates / Q000378:metabolism; D051193:Toll-Like Receptors / Q000235:genetics / Q000378:metabolism
chemical_list: D029941:Fish Proteins; D050839:Interferon Regulatory Factor-7; D008351:Mannans; D053594:Myeloid Differentiation Factor 88; D013431:Sulfates; D051193:Toll-Like Receptors; C022901:(1-6)-alpha-glucomannan
keywords: Acidolysis-oxidized konjac glucomannan; Immunity; Interferon regulatory factors7; Myeloid differentiation factor 88; Oxidized konjac glucomannan sulfates; Toll-like receptor 22