title: A Comparison of Biocompatibility of a Titanium Alloy Fabricated by Electron Beam Melting and Selective Laser Melting.
abstract: Electron beam melting (EBM) and selective laser melting (SLM) are two advanced rapid prototyping manufacturing technologies capable of fabricating complex structures and geometric shapes from metallic materials using computer tomography (CT) and Computer-aided Design (CAD) data. Compared to traditional technologies used for metallic products, EBM and SLM alter the mechanical, physical and chemical properties, which are closely related to the biocompatibility of metallic products. In this study, we evaluate and compare the biocompatibility, including cytocompatibility, haemocompatibility, skin irritation and skin sensitivity of Ti6Al4V fabricated by EBM and SLM. The results were analysed using one-way ANOVA and Tukey's multiple comparison test. Both the EBM and SLM Ti6Al4V exhibited good cytobiocompatibility. The haemolytic ratios of the SLM and EBM were 2.24% and 2.46%, respectively, which demonstrated good haemocompatibility. The EBM and SLM Ti6Al4V samples showed no dermal irritation when exposed to rabbits. In a delayed hypersensitivity test, no skin allergic reaction from the EBM or the SLM Ti6Al4V was observed in guinea pigs. Based on these results, Ti6Al4V fabricated by EBM and SLM were good cytobiocompatible, haemocompatible, non-irritant and non-sensitizing materials. Although the data for cell adhesion, proliferation, ALP activity and the haemolytic ratio was higher for the SLM group, there were no significant differences between the different manufacturing methods.
authors.affiliation: Department of Stomatology, General Hospital of the People's Libration Army, Beijing, China.|Department of Stomatology, the Second Affiliated Stomatological Hospital of Liaoning Medical University, Jinzhou, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Stomatology, General Hospital of the People's Libration Army, Beijing, China.|Department of Stomatology, the Second Affiliated Stomatological Hospital of Liaoning Medical University, Jinzhou, China.
authors.forename: Bingjing
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Department of Stomatology, the 451th Hospital of the People's Libration Army, Xi'an, China.
authors.forename: Changkui
authors.initials: C
authors.lastname: Liu
authors.affiliation: School of Medicine, Nankai University, Tianjin, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Stomatology, the 304th Hospital of the People's Libration Army, Beijing, China.
authors.forename: Xinying
authors.initials: X
authors.lastname: Tan
authors.affiliation: Department of Stomatology, General Hospital of the People's Libration Army, Beijing, China.
authors.forename: Min
authors.initials: M
authors.lastname: Hu
mesh_terms: D000497:Alloys / Q000009:adverse effects / Q000737:chemistry* / Q000494:pharmacology; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004285:Dogs; D004583:Electrons; D006168:Guinea Pigs; D007834:Lasers; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis / Q000187:drug effects; D011817:Rabbits; D012867:Skin / Q000187:drug effects; D013499:Surface Properties; D014025:Titanium / Q000009:adverse effects / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000497:Alloys; C031462:titanium alloy (TiAl6V4); D014025:Titanium