title: Characterization of irritans mariner-like elements in the olive fruit fly Bactrocera oleae (Diptera: Tephritidae): evolutionary implications.
abstract: Genomic variation among species is commonly driven by transposable element (TE) invasion; thus, the pattern of TEs in a genome allows drawing an evolutionary history of the studied species. This paper reports in vitro and in silico detection and characterization of irritans mariner-like elements (MLEs) in the genome and transcriptome of Bactrocera oleae (Rossi) (Diptera: Tephritidae). Eleven irritans MLE sequences have been isolated in vitro using terminal inverted repeats (TIRs) as primers, and 215 have been extracted in silico from the sequenced genome of B. oleae. Additionally, the sequenced genomes of Bactrocera tryoni (Froggatt) and Bactrocera cucurbitae (Diptera: Tephritidae) have been explored to identify irritans MLEs. A total of 129 sequences from B. tryoni have been extracted, while the genome of B. cucurbitae appears probably devoid of irritans MLEs. All detected irritans MLEs are defective due to several mutations and are clustered together in a monophyletic group suggesting a common ancestor. The evolutionary history and dynamics of these TEs are discussed in relation with the phylogenetic distribution of their hosts. The knowledge on the structure, distribution, dynamic, and evolution of irritans MLEs in Bactrocera species contributes to the understanding of both their evolutionary history and the invasion history of their hosts. This could also be the basis for genetic control strategies using transposable elements.
authors.affiliation: UR Génomique des Insectes Ravageurs des Cultures d'Intérêt Agronomique (UR11ES10), Faculté des Sciences de Tunis, Université de Tunis El-Manar, 2092, El Manar, Tunisia.
authors.forename: Wafa
authors.initials: W
authors.lastname: Ben Lazhar-Ajroud
authors.affiliation: Laboratoire Mer-Molécules-Santé (EA 2160 MMS), Université du Maine, Avenue Olivier Messiaen, 72085, Le Mans, France.
authors.forename: Aurore
authors.initials: A
authors.lastname: Caruso
authors.affiliation: UR Génomique des Insectes Ravageurs des Cultures d'Intérêt Agronomique (UR11ES10), Faculté des Sciences de Tunis, Université de Tunis El-Manar, 2092, El Manar, Tunisia.
authors.forename: Maha
authors.initials: M
authors.lastname: Mezghani
authors.affiliation: UR Génomique des Insectes Ravageurs des Cultures d'Intérêt Agronomique (UR11ES10), Faculté des Sciences de Tunis, Université de Tunis El-Manar, 2092, El Manar, Tunisia.|Laboratoire EGCE, UPR 9034 CNRS, Avenue de la Terrasse, 91198, Gif-sur-Yvette, France.
authors.forename: Maryem
authors.initials: M
authors.lastname: Bouallegue
authors.affiliation: Laboratoire Mer-Molécules-Santé (EA 2160 MMS), Université du Maine, Avenue Olivier Messiaen, 72085, Le Mans, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Tastard
authors.affiliation: Laboratoire Mer-Molécules-Santé (EA 2160 MMS), Université du Maine, Avenue Olivier Messiaen, 72085, Le Mans, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Denis
authors.affiliation: Laboratoire EGCE, UPR 9034 CNRS, Avenue de la Terrasse, 91198, Gif-sur-Yvette, France.
authors.forename: Jacques-Deric
authors.initials: JD
authors.lastname: Rouault
authors.affiliation: UR Génomique des Insectes Ravageurs des Cultures d'Intérêt Agronomique (UR11ES10), Faculté des Sciences de Tunis, Université de Tunis El-Manar, 2092, El Manar, Tunisia.|Institut Supérieur de l'Animation pour la Jeunesse et la Culture de Bir-El-Bey, Université de Tunis, 2055, Bir El Bey, Tunisia.
authors.forename: Hanem
authors.initials: H
authors.lastname: Makni
authors.affiliation: Laboratoire EGCE, UPR 9034 CNRS, Avenue de la Terrasse, 91198, Gif-sur-Yvette, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Capy
authors.affiliation: Laboratoire Mer-Molécules-Santé (EA 2160 MMS), Université du Maine, Avenue Olivier Messiaen, 72085, Le Mans, France. bchenais@univ-lemans.fr.
authors.forename: Benoît
authors.identifier: http://orcid.org/0000-0003-2441-1966
authors.initials: B
authors.lastname: Chénais
authors.affiliation: UR Génomique des Insectes Ravageurs des Cultures d'Intérêt Agronomique (UR11ES10), Faculté des Sciences de Tunis, Université de Tunis El-Manar, 2092, El Manar, Tunisia.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Makni
authors.affiliation: Laboratoire Mer-Molécules-Santé (EA 2160 MMS), Université du Maine, Avenue Olivier Messiaen, 72085, Le Mans, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Casse
mesh_terms: D000818:Animals; D003198:Computer Simulation; D004251:DNA Transposable Elements / Q000235:genetics*; D049750:Genome, Insect / Q000235:genetics*; D009154:Mutation; D010802:Phylogeny; D033621:Tephritidae / Q000145:classification* / Q000235:genetics*; D020079:Terminal Repeat Sequences / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements
keywords: Bactrocera genus; Bactrocera oleae; Irritans mariner-like elements; Transposons