title: Carbon ion irradiation withstands cancer stem cells' migration/invasion process in Head and Neck Squamous Cell Carcinoma (HNSCC).
abstract: Cancer Stem Cells (CSCs) in Head and Neck Squamous Cell Carcinoma (HNSCC) have extremely aggressive profile (high migratory and invasive potential). These characteristics can explain their resistance to conventional treatment. Efficacy of photon and carbon ion irradiation with addition of cetuximab (5 nM) is studied on clonogenic death, migration and invasion of two HNSCC populations: SQ20B and SQ20B/CSCs. SQ20B express E-cadherin and overexpress EGFR while SQ20B/CSCs express N-cadherin and low EGFR. Cetuximab strongly inhibits SQ20B proliferation but has no effect on SQ20B/CSCs. 2 Gy photon irradiation enhances migration and invasiveness in both populations (p < 0.05), while cetuximab only stops SQ20B migration (p < 0.005). Carbon irradiation significantly inhibits invasion in both populations (p < 0.05), and the association with cetuximab significantly inhibits invasion in both populations (p < 0.005). These results highlight CSCs characteristics: EGFRLow, cetuximab-resistant, and highly migratory. Carbon ion irradiation appears to be a very promising therapeutic modality counteracting migration/invasion process in both parental cells and CSCs in contrast to photon irradiation.
authors.affiliation: Université Lyon 1, Faculté de Médecine-Lyon-Sud, Oullins, 69921, France.|Laboratoire de Radiobiologie Cellulaire et Moléculaire, Institut de Physique Nucléaire de Lyon, IPNL, Villeurbanne, 69622, France.|Département de Radiothérapie, Institut de Cancérologie de la Loire - Lucien Neuwirth, St Priest en Jarez, 42270, France.
authors.forename: Coralie
authors.initials: C
authors.lastname: Moncharmont
authors.affiliation: Université Lyon 1, Faculté de Médecine-Lyon-Sud, Oullins, 69921, France.|Laboratoire de Radiobiologie Cellulaire et Moléculaire, Institut de Physique Nucléaire de Lyon, IPNL, Villeurbanne, 69622, France.|Département de Radiothérapie, Institut de Cancérologie de la Loire - Lucien Neuwirth, St Priest en Jarez, 42270, France.
authors.forename: Jean-Baptiste
authors.initials: JB
authors.lastname: Guy
authors.affiliation: Université Lyon 1, Faculté de Médecine-Lyon-Sud, Oullins, 69921, France.|Laboratoire de Radiobiologie Cellulaire et Moléculaire, Institut de Physique Nucléaire de Lyon, IPNL, Villeurbanne, 69622, France.|Hospices Civils de Lyon, Lyon, 69229, France.
authors.forename: Anne-Sophie
authors.initials: AS
authors.lastname: Wozny
authors.affiliation: Université Lyon 1, Faculté de Médecine-Lyon-Sud, Oullins, 69921, France.|Laboratoire de Radiobiologie Cellulaire et Moléculaire, Institut de Physique Nucléaire de Lyon, IPNL, Villeurbanne, 69622, France.
authors.forename: Marion
authors.initials: M
authors.lastname: Gilormini
authors.affiliation: Université Lyon 1, Faculté de Médecine-Lyon-Sud, Oullins, 69921, France.|Laboratoire de Radiobiologie Cellulaire et Moléculaire, Institut de Physique Nucléaire de Lyon, IPNL, Villeurbanne, 69622, France.
authors.forename: Priscilla
authors.initials: P
authors.lastname: Battiston-Montagne
authors.affiliation: Université Lyon 1, Faculté de Médecine-Lyon-Sud, Oullins, 69921, France.|Laboratoire de Radiobiologie Cellulaire et Moléculaire, Institut de Physique Nucléaire de Lyon, IPNL, Villeurbanne, 69622, France.|Hospices Civils de Lyon, Lyon, 69229, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Ardail
authors.affiliation: Institut de Physique Nucléaire de Lyon, IPNL, Villeurbanne, 69622, France.
authors.forename: Michael
authors.initials: M
authors.lastname: Beuve
authors.affiliation: Laboratoire de Radiobiologie Cellulaire et Moléculaire, Institut de Physique Nucléaire de Lyon, IPNL, Villeurbanne, 69622, France.|Hospices Civils de Lyon, Lyon, 69229, France.
authors.forename: Gersende
authors.initials: G
authors.lastname: Alphonse
authors.affiliation: Département de Pharmacologie Clinique et d'Innovation, Institut de Cancérologie de la Loire - Lucien Neuwirth, St Priest en Jarez, 42270, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Simoëns
authors.affiliation: Département de Radiothérapie, Institut de Cancérologie de la Loire - Lucien Neuwirth, St Priest en Jarez, 42270, France.
authors.forename: Chloé
authors.initials: C
authors.lastname: Rancoule
authors.affiliation: Université Lyon 1, Faculté de Médecine-Lyon-Sud, Oullins, 69921, France.|Laboratoire de Radiobiologie Cellulaire et Moléculaire, Institut de Physique Nucléaire de Lyon, IPNL, Villeurbanne, 69622, France.|Hospices Civils de Lyon, Lyon, 69229, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Rodriguez-Lafrasse
authors.affiliation: Université Lyon 1, Faculté de Médecine-Lyon-Sud, Oullins, 69921, France.|Laboratoire de Radiobiologie Cellulaire et Moléculaire, Institut de Physique Nucléaire de Lyon, IPNL, Villeurbanne, 69622, France.|Département de Radiothérapie, Institut de Cancérologie de la Loire - Lucien Neuwirth, St Priest en Jarez, 42270, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Magné
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000188:drug therapy / Q000473:pathology* / Q000532:radiotherapy*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects / Q000528:radiation effects; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D000068818:Cetuximab / Q000494:pharmacology; D059248:Chemoradiotherapy; D058750:Epithelial-Mesenchymal Transition; D066246:ErbB Receptors / Q000096:biosynthesis; D006258:Head and Neck Neoplasms / Q000188:drug therapy / Q000473:pathology* / Q000532:radiotherapy*; D063193:Heavy Ion Radiotherapy; D006801:Humans; D009361:Neoplasm Invasiveness; D014411:Neoplastic Stem Cells / Q000187:drug effects / Q000473:pathology* / Q000528:radiation effects*; D017785:Photons / Q000627:therapeutic use; D000077195:Squamous Cell Carcinoma of Head and Neck; D016019:Survival Analysis
chemical_list: C512478:EGFR protein, human; D066246:ErbB Receptors; D000068818:Cetuximab
keywords: HNSCC; cancer stem cells; carbon ion irradiation; invasion; migration