title: PCP4/PEP19 promotes migration, invasion and adhesion in human breast cancer MCF-7 and T47D cells.
abstract: Purkinje cell protein (PCP) 4/peptide (PEP) 19 is expressed in Purkinje cells where it has a calmodulin-binding, anti-apoptotic function. We recently demonstrated that PCP4/PEP19 is expressed and inhibit apoptosis in human breast cancer cell lines. In the present study we investigated the role of PCP4/PEP19 in cell morphology, adhesion, migration, and invasion in MCF-7 and T47D human breast cancer cell lines. Knockdown of PCP4/PEP19 reduced the formation of filopodia-like cytoplasmic structures and vinculin expression, and enhanced E-cadherin expression. Activities of migration, invasion, and cell adhesion were also decreased after the knockdown of PCP4/PEP19 in MCF-7 and T47D cells. These results suggested that PCP4/PEP19 promotes cancer cell adhesion, migration, and invasion and that PCP4/PEP19 may be a potential target for therapeutic agents in breast cancer treatment which act by inhibiting epithelial-mesenchymal transition and enhancing apoptotic cell death.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
authors.forename: Takuya
authors.initials: T
authors.lastname: Yoshimura
authors.affiliation: Department of Pathology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
authors.forename: Taiji
authors.initials: T
authors.lastname: Hamada
authors.affiliation: Department of Oral and Maxillofacial Surgery, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hijioka
authors.affiliation: Department of Pathology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
authors.forename: Masakazu
authors.initials: M
authors.lastname: Souda
authors.affiliation: Department of Pathology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Hatanaka
authors.affiliation: Department of Pathology, Children's Cancer Center, National Center for Child Health and Development, Tokyo, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Yoshioka
authors.affiliation: Department of Pathology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
authors.forename: Sohsuke
authors.initials: S
authors.lastname: Yamada
authors.affiliation: Department of Pharmacology, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Tsutsui
authors.affiliation: Division of Organ Pathology, Faculty of Medicine, Tottori University, Yonago, Japan.
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Umekita
authors.affiliation: Department of Oral and Maxillofacial Surgery, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
authors.forename: Norifumi
authors.initials: N
authors.lastname: Nakamura
authors.affiliation: Department of Pathology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
authors.forename: Akihide
authors.initials: A
authors.lastname: Tanimoto
mesh_terms: D017209:Apoptosis; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology*; D015820:Cadherins / Q000378:metabolism; D002448:Cell Adhesion; D002452:Cell Count; D045744:Cell Line, Tumor; D002465:Cell Movement; D003470:Culture Media; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D061986:MCF-7 Cells; D009361:Neoplasm Invasiveness; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D063150:Polycomb Repressive Complex 1 / Q000378:metabolism; D011554:Pseudopodia / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D016896:Treatment Outcome; D016596:Vinculin / Q000378:metabolism
chemical_list: C069077:BMI1 protein, human; D015820:Cadherins; D003470:Culture Media; D009419:Nerve Tissue Proteins; D034741:RNA, Small Interfering; C050394:PCP4 protein, human; D016596:Vinculin; D063150:Polycomb Repressive Complex 1
keywords: EMT; PCP4/PEP19; breast cancer; invasion; migration