title: Downregulation of human Wnt3 in gastric cancer suppresses cell proliferation and induces apoptosis.
abstract: Aberrant activation of Wnt/β-catenin signaling pathways is closely involved in the occurrence and progression of several types of human malignancies. However, as a fundamental component in this cascade, Wnt3 has not been well understood for the expression level and pathogenic mechanism in gastric carcinogenesis. Here, this research was undertaken to elucidate the important role of Wnt3 in gastric cancer. Wnt3 expression in gastric carcinomas and their respective normal tissues was examined by immunoblotting and immunohistochemistry. In all cases, Wnt3 expression was significantly elevated in gastric carcinomas compared with normal tissues. Knocking down Wnt3 in MGC-803 gastric cancer cells by small interfering RNAs transfection led to an obvious decrease in both transcript and protein levels. Silence of Wnt3 expression in gastric cancer cells inhibited the expression of β-catenin and cyclin D1 genes in Wnt/β-catenin pathway, significantly blocked cellular proliferation, delayed cell cycle, suppressed cell invasion and metastasis, accompanied by a higher apoptosis rate. Together, we conclude that upregulation of Wnt3 plays a crucial role in gastric tumorigenesis by inducing proliferation, migration, and invasion and inhibiting apoptosis of cancer cells, and Wnt3 might be a potential target for the treatment of gastric cancer.
authors.affiliation: Key Laboratory of Molecular Pathology, School of Basic Medical Science, Inner Mongolia Medical University, Hohhot.
authors.forename: Hai-Sheng
authors.initials: HS
authors.lastname: Wang
authors.affiliation: Key Laboratory of Receptors-Mediated Gene Regulation and Drug Discovery, School of Medicine, Henan University, Kaifeng.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Nie
authors.affiliation: Key Laboratory of Molecular Pathology, School of Basic Medical Science, Inner Mongolia Medical University, Hohhot.
authors.forename: Rui-Bing
authors.initials: RB
authors.lastname: Wu
authors.affiliation: Key Laboratory of Molecular Pathology, School of Basic Medical Science, Inner Mongolia Medical University, Hohhot.
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Yuan
authors.affiliation: Key Laboratory of Molecular Pathology, School of Basic Medical Science, Inner Mongolia Medical University, Hohhot.
authors.forename: Yue-Hong
authors.initials: YH
authors.lastname: Ma
authors.affiliation: Key Laboratory of Molecular Pathology, School of Basic Medical Science, Inner Mongolia Medical University, Hohhot.
authors.forename: Xiu-Lan
authors.initials: XL
authors.lastname: Liu
authors.affiliation: Key Laboratory of Molecular Pathology, School of Basic Medical Science, Inner Mongolia Medical University, Hohhot.
authors.forename: Jian-Yu
authors.initials: JY
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Molecular Pathology, School of Basic Medical Science, Inner Mongolia Medical University, Hohhot.
authors.forename: Xiu-Ling
authors.initials: XL
authors.lastname: Deng
authors.affiliation: Key Laboratory of Molecular Pathology, School of Basic Medical Science, Inner Mongolia Medical University, Hohhot.
authors.forename: Qin
authors.initials: Q
authors.lastname: Na
authors.affiliation: Key Laboratory of Molecular Pathology, School of Basic Medical Science, Inner Mongolia Medical University, Hohhot.
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Jin
authors.affiliation: Key Laboratory of Molecular Pathology, School of Basic Medical Science, Inner Mongolia Medical University, Hohhot.
authors.forename: Yan-Chao
authors.initials: YC
authors.lastname: Bian
authors.affiliation: Epidemiology Section, Public Health School, Inner Mongolia Medical University, Hohhot.
authors.forename: Yu-Min
authors.initials: YM
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, People's Republic of China.
authors.forename: Yan-Dong
authors.initials: YD
authors.lastname: Wang
authors.affiliation: Key Laboratory of Molecular Pathology, School of Basic Medical Science, Inner Mongolia Medical University, Hohhot; Key Laboratory of Receptors-Mediated Gene Regulation and Drug Discovery, School of Medicine, Henan University, Kaifeng.
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Chen
keywords: gastric carcinogenesis; gastric carcinoma; invasion; siRNA; β-catenin