title: Self-assembly of c-myc DNA promoted by a single enantiomer ruthenium complex as a potential nuclear targeting gene carrier.
abstract: Gene therapy has long been limited in the clinic, due in part to the lack of safety and efficacy of the gene carrier. Herein, a single enantiomer ruthenium(II) complex, Λ-[Ru(bpy)2(p-BEPIP)](ClO4)2 (Λ-RM0627, bpy = 4,4'-bipyridine, p-BEPIP = 2-(4-phenylacetylenephenyl)imidazole [4,5f][1, 10] phenanthroline), has been synthesized and investigated as a potential gene carrier that targets the nucleus. In this report, it is shown that Λ-RM0627 promotes self-assembly of c-myc DNA to form a nanowire structure. Further studies showed that the nano-assembly of c-myc DNA that induced Λ-RM0627 could be efficiently taken up and enriched in the nuclei of HepG2 cells. After treatment of the nano-assembly of c-myc DNA with Λ-RM0627, over-expression of c-myc in HepG2 cells was observed. In summary, Λ-RM0627 played a key role in the transfer and release of c-myc into cells, which strongly indicates Λ-RM0627 as a potent carrier of c-myc DNA that targets the nucleus of tumor cells.
authors.affiliation: School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.affiliation: School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Mei
authors.affiliation: Traditional Chinese Medicine College, Guangdong Pharmaceutical University, Guangzhou 510006, China.
authors.forename: Kangdi
authors.initials: K
authors.lastname: Zheng
authors.affiliation: School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Ding
mesh_terms: D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D054856:G-Quadruplexes / Q000187:drug effects; D018390:Gene Targeting; D056945:Hep G2 Cells; D006801:Humans; D018613:Microscopy, Confocal; D049329:Nanostructures / Q000737:chemistry; D053770:Nanowires / Q000737:chemistry; D010618:Phenanthrolines / Q000737:chemistry; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics* / Q000378:metabolism; D011725:Pyridines / Q000737:chemistry; D012428:Ruthenium / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D056831:Coordination Complexes; D010618:Phenanthrolines; D016271:Proto-Oncogene Proteins c-myc; D011725:Pyridines; D012428:Ruthenium; D004247:DNA; C034306:4,4'-bipyridyl