title: Mechanism of the cytotoxic effect of l-amino acid oxidase isolated from Bothrops alternatus snake venom.
abstract: BaltLAAO-I, an L-amino acid oxidase isolated from Bothrops alternatus, is a glycoprotein enzyme with a pI-5.3, 15% sugar and a related molecular mass of 66,000Da in its monomeric form, and 123,000Da in its dimeric form. The objective of this study is to describe the cytotoxicity activity induced by BaltLAAO-I isolated from Bothrops alternatus venom and its possible mechanism of action on tumor cells. Our results clearly depict that BaltLAAO-I has a strong selective cytotoxic activity on tumor cell lines (JURKAT, SK-BR-3 and B16F10). On the other hand, the results show low cytotoxicity on human peripheral blood mononuclear cells. Furthermore, our findings demonstrate that BaltLAAO-I induces the apoptosis of tumor cell lines through a cytotoxic activity exerted by a generation of reactive oxygen intermediates. All in all, the data indicate that LAAOs exert a selective cytotoxic role on tumor cells, demonstrating a great potential for future use in clinical therapy.
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, FCFRP-USP, Ribeirão Preto-SP, Brazil.
authors.forename: Patrícia H
authors.initials: PH
authors.lastname: Ribeiro
authors.affiliation: Centro de Estudos de Biomoléculas Aplicadas à Saúde, CEBio, Fundação Oswaldo Cruz, FIOCRUZ, Fiocruz Rondônia e Departamento de Medicina, Universidade Federal de Rondônia, UNIR-RO, Brazil.
authors.forename: Juliana P
authors.initials: JP
authors.lastname: Zuliani
authors.affiliation: Centro de Estudos de Biomoléculas Aplicadas à Saúde, CEBio, Fundação Oswaldo Cruz, FIOCRUZ, Fiocruz Rondônia e Departamento de Medicina, Universidade Federal de Rondônia, UNIR-RO, Brazil; Centro de Pesquisa em Medicina Tropical (CEPEM), Porto Velho-RO, Brazil.
authors.forename: Carla F C
authors.initials: CF
authors.lastname: Fernandes
authors.affiliation: Centro de Estudos de Biomoléculas Aplicadas à Saúde, CEBio, Fundação Oswaldo Cruz, FIOCRUZ, Fiocruz Rondônia e Departamento de Medicina, Universidade Federal de Rondônia, UNIR-RO, Brazil.
authors.forename: Leonardo A
authors.initials: LA
authors.lastname: Calderon
authors.affiliation: Centro de Estudos de Biomoléculas Aplicadas à Saúde, CEBio, Fundação Oswaldo Cruz, FIOCRUZ, Fiocruz Rondônia e Departamento de Medicina, Universidade Federal de Rondônia, UNIR-RO, Brazil.
authors.forename: Rodrigo G
authors.initials: RG
authors.lastname: Stábeli
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, FCFRP-USP, Ribeirão Preto-SP, Brazil. Electronic address: aunomizo@fcfrp.usp.br.
authors.forename: Auro
authors.initials: A
authors.lastname: Nomizo
authors.affiliation: Centro de Estudos de Biomoléculas Aplicadas à Saúde, CEBio, Fundação Oswaldo Cruz, FIOCRUZ, Fiocruz Rondônia e Departamento de Medicina, Universidade Federal de Rondônia, UNIR-RO, Brazil; Faculdade São Lucas, FSL, Porto Velho-RO, Brazil. Electronic address: andreimar@fiocruz.br.
authors.forename: Andreimar M
authors.initials: AM
authors.lastname: Soares
mesh_terms: D000328:Adult; D000818:Animals; D017837:Bothrops / Q000378:metabolism*; D002374:Catalase / Q000494:pharmacology; D016923:Cell Death / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D004247:DNA / Q000032:analysis; D053938:DNA Fragmentation / Q000187:drug effects; D006801:Humans; D019169:Jurkat Cells; D050830:L-Amino Acid Oxidase / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012910:Snake Venoms / Q000201:enzymology*; D013194:Staining and Labeling; D055815:Young Adult
chemical_list: D017382:Reactive Oxygen Species; D012910:Snake Venoms; D004247:DNA; D002374:Catalase; D050830:L-Amino Acid Oxidase
keywords: Apoptosis; Bothrops alternatus; LAAO