title: Borrelia burgdorferi sensu lato in Ixodes cf. neuquenensis and Ixodes sigelos ticks from the Patagonian region of Argentina.
abstract: This study was conducted to detect Borrelia burgdorferi sensu lato infection in ixodid ticks from the Patagonia region in the south of Argentina. Therefore, ticks were collected on rodents in the provinces of Chubut, Río Negro and Santa Cruz. These ticks were identified as nymphs of Ixodes cf. neuquenensis and Ixodes sigelos. The B. burgdorferi s.l. infection was tested by a battery of PCR methods targeting the gene flagellin (fla) and the rrfA-rrlB intergenic spacer region (IGS). Three pools of I. sigelos nymphs from Chubut and Santa Cruz provinces as well as one pool of I. cf. neuquenensis nymphs from Río Negro province were tested positive in the fla-PCR. The samples of I. sigelos were also positive for the IGS-PCR. Phylogenetically, the haplotypes found in the positive ticks belong to the B. burgdorferi s.l. complex, and they were closely related to Borrelia chilensis, a genospecies isolated from Ixodes stilesi in Chile. The pathogenic relevance of the Borrelia genospecies detected in both I. neuquenensis and I. sigelos is unknown.
authors.affiliation: Parasitology Unit, University of Hohenheim, Emil-Wolff-Straße 34, 70593 Stuttgart, Germany; Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Rafaela and Consejo Nacional de Investigaciones Científicas y Técnicas, CC 22, 2300 Rafaela, Santa Fe, Argentina. Electronic address: patrick.sebastian@uni-hohenheim.de.
authors.forename: Patrick S
authors.initials: PS
authors.lastname: Sebastian
authors.affiliation: Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Rafaela and Consejo Nacional de Investigaciones Científicas y Técnicas, CC 22, 2300 Rafaela, Santa Fe, Argentina.
authors.forename: Maria Noelia Saracho
authors.initials: MNS
authors.lastname: Bottero
authors.affiliation: Laboratorio de Vectores y Enfermedades Transmitidas, Facultad de Veterinaria, CENUR Litoral Norte-Salto, Universidad de la República, Rivera 1350, CP 50000 Salto, Uruguay.
authors.forename: Luis
authors.initials: L
authors.lastname: Carvalho
authors.affiliation: Parasitology Unit, University of Hohenheim, Emil-Wolff-Straße 34, 70593 Stuttgart, Germany.
authors.forename: Ute
authors.initials: U
authors.lastname: Mackenstedt
authors.affiliation: Centro de Estudios Parasitológicos y de Vectores (CONICET-UNLP) La Plata, Buenos Aires, Argentina.
authors.forename: Marcela
authors.initials: M
authors.lastname: Lareschi
authors.affiliation: Laboratorio de Vectores y Enfermedades Transmitidas, Facultad de Veterinaria, CENUR Litoral Norte-Salto, Universidad de la República, Rivera 1350, CP 50000 Salto, Uruguay.
authors.forename: José M
authors.initials: JM
authors.lastname: Venzal
authors.affiliation: Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Rafaela and Consejo Nacional de Investigaciones Científicas y Técnicas, CC 22, 2300 Rafaela, Santa Fe, Argentina.
authors.forename: Santiago
authors.initials: S
authors.lastname: Nava
mesh_terms: D000818:Animals; D001118:Argentina / Q000453:epidemiology; D025065:Borrelia burgdorferi / Q000235:genetics*; D002677:Chile; D004269:DNA, Bacterial / Q000235:genetics; D021901:DNA, Intergenic; D018884:Ixodes / Q000382:microbiology*; D008193:Lyme Disease / Q000235:genetics*; D009758:Nymph / Q000382:microbiology; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D004269:DNA, Bacterial; D021901:DNA, Intergenic
keywords: Argentina; Borrelia burgdorferi sensu lato; Ixodes cf. neuquenensis; Ixodes sigelos; Patagonia