title: Serum Proteomics Analysis in Rats of Immunosuppression Induced by Chronic Stress.
abstract: The immune system can be damaged by chronic stress. However, for this process, the involved molecular alterations and their regulatory roles played in immunosuppression still remain unclear. This study was aimed to identify the differences in serum protein expressions that are closely associated with the effect of chronic stress on immune function. Serum protein levels of rats in control group and chronic stress group were measured by iTRAQ analysis. Subsequently, among the 121 differentially expressed proteins screened between the two groups, 46 proteins were upregulated (>1.5-fold, P < 0.05), while 75 proteins were downregulated (<0.67-fold, P < 0.05). Bioinformatics analysis revealed that most of the differentially expressed proteins were in relation with the metabolic, cellular, response stimulus and immune system processes. The significantly differential expression of ceruloplasmin, haptoglobin, catalase and peroxiredoxin-1 were picked out for reconfirmation by ELISA analysis. The results were consistent with those obtained by iTRAQ. What is more, the roles of above-mentioned four proteins, apolipoprotein B-100 and heat-shock protein 90 in immunosuppression induced by chronic stress were discussed. Taken together, these findings may provide a new insight into better understanding the molecular mechanisms of immunosuppression induced by chronic stress.
authors.affiliation: Basic Medical Science College, Liaoning University of Traditional Chinese Medicine, Shenyang, Liaoning, P. R. China.|Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications, Liaoning University of Traditional Chinese Medicine, Shenyang, China.
authors.forename: D D
authors.initials: DD
authors.lastname: Hou
authors.affiliation: Shengjing Hospital, China Medical University, Shenyang, 110004, China.
authors.forename: R Z
authors.initials: RZ
authors.lastname: Zhu
authors.affiliation: Liaoning People's Hospital, Shenyang, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Experiment and Technology Center, Liaoning University of Traditional Chinese Medicine, Shenyang, P. R. China.
authors.forename: X D
authors.initials: XD
authors.lastname: Ma
authors.affiliation: The Second Clinical Medical Institute, Liaoning University of Traditional Chinese Medicine, Shenyang, P. R. China.
authors.forename: D C
authors.initials: DC
authors.lastname: Wang
authors.affiliation: The First Clinical Medical Institute, Liaoning University of Traditional Chinese Medicine, Shenyang, P. R. China.
authors.forename: H
authors.initials: H
authors.lastname: Timothy
authors.affiliation: Experiment and Technology Center, Liaoning University of Traditional Chinese Medicine, Shenyang, P. R. China.
authors.forename: W N
authors.initials: WN
authors.lastname: Chen
authors.affiliation: The First Clinical Medical Institute, Liaoning University of Traditional Chinese Medicine, Shenyang, P. R. China.
authors.forename: F
authors.initials: F
authors.lastname: Yan
authors.affiliation: Basic Medical Science College, Liaoning University of Traditional Chinese Medicine, Shenyang, Liaoning, P. R. China.|Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications, Liaoning University of Traditional Chinese Medicine, Shenyang, China.
authors.forename: P
authors.initials: P
authors.lastname: Lei
authors.affiliation: Basic Medical Science College, Liaoning University of Traditional Chinese Medicine, Shenyang, Liaoning, P. R. China.|Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications, Liaoning University of Traditional Chinese Medicine, Shenyang, China.
authors.forename: X W
authors.initials: XW
authors.lastname: Han
authors.affiliation: Basic Medical Science College, Liaoning University of Traditional Chinese Medicine, Shenyang, Liaoning, P. R. China.|Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications, Liaoning University of Traditional Chinese Medicine, Shenyang, China.
authors.forename: D X
authors.initials: DX
authors.lastname: Chen
authors.affiliation: Basic Medical Science College, Liaoning University of Traditional Chinese Medicine, Shenyang, Liaoning, P. R. China.|Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications, Liaoning University of Traditional Chinese Medicine, Shenyang, China.
authors.forename: L P
authors.initials: LP
authors.lastname: Cai
authors.affiliation: Basic Medical Science College, Liaoning University of Traditional Chinese Medicine, Shenyang, Liaoning, P. R. China.|Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications, Liaoning University of Traditional Chinese Medicine, Shenyang, China.
authors.forename: H Q
authors.initials: HQ
authors.lastname: Guan
mesh_terms: D000818:Animals; D053299:Apolipoprotein B-100 / Q000097:blood / Q000235:genetics / Q000276:immunology; D002374:Catalase / Q000097:blood / Q000235:genetics / Q000276:immunology; D002570:Ceruloplasmin / Q000235:genetics / Q000276:immunology; D019295:Computational Biology / Q000379:methods; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000276:immunology*; D018841:HSP90 Heat-Shock Proteins / Q000097:blood / Q000235:genetics / Q000276:immunology; D006242:Haptoglobins / Q000235:genetics / Q000276:immunology; D007103:Immobilization; D007165:Immunosuppression Therapy; D007694:Killer Cells, Natural / Q000737:chemistry / Q000276:immunology; D054464:Peroxiredoxins / Q000097:blood / Q000235:genetics / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar; D013315:Stress, Psychological / Q000235:genetics* / Q000276:immunology; D013550:Swimming
chemical_list: D053299:Apolipoprotein B-100; D018841:HSP90 Heat-Shock Proteins; D006242:Haptoglobins; C489898:Prdx1 protein, rat; D054464:Peroxiredoxins; D002374:Catalase; D002570:Ceruloplasmin