title: Identification of secreted and membrane-bound bat immunoglobulin using a Microchiropteran-specific mouse monoclonal antibody.
abstract: Bat immunity has received increasing attention because some bat species are being decimated by the fungal disease, White Nose Syndrome, while other species are potential reservoirs of zoonotic viruses. Identifying specific immune processes requires new specific tools and reagents. In this study, we describe a new mouse monoclonal antibody (mAb) reactive with Eptesicus fuscus immunoglobulins. The epitope recognized by mAb BT1-4F10 was localized to immunoglobulin light (lambda) chains; hence, the mAb recognized serum immunoglobulins and B lymphocytes. The BT1-4F10 epitope appeared to be restricted to Microchiropteran immunoglobulins and absent from Megachiropteran immunoglobulins. Analyses of sera and other E. fuscus fluids showed that most, if not all, secreted immunoglobulins utilized lambda light chains. Finally, mAb BT1-4F10 permitted the identification of B cell follicles in splenic white pulp. This Microchiropteran-specific mAb has potential utility in seroassays; hence, this reagent may have both basic and practical applications for studying immune process.
authors.affiliation: The Department of Biomedical Sciences, The School of Public Health, The University at Albany, Albany, NY, 12201-0509, USA; The Laboratory of Immunology, The Wadsworth Center, New York State Department of Health, Albany, NY, 12201-2002, USA. Electronic address: William.lee@health.ny.gov.
authors.forename: William T
authors.initials: WT
authors.lastname: Lee
authors.affiliation: The Department of Biomedical Sciences, The School of Public Health, The University at Albany, Albany, NY, 12201-0509, USA.
authors.forename: Derek D
authors.initials: DD
authors.lastname: Jones
authors.affiliation: The Department of Biomedical Sciences, The School of Public Health, The University at Albany, Albany, NY, 12201-0509, USA.
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Yates
authors.affiliation: The Department of Biomedical Sciences, The School of Public Health, The University at Albany, Albany, NY, 12201-0509, USA; The Laboratory of Immunology, The Wadsworth Center, New York State Department of Health, Albany, NY, 12201-2002, USA.
authors.forename: Gary M
authors.initials: GM
authors.lastname: Winslow
authors.affiliation: The Laboratory of Immunology, The Wadsworth Center, New York State Department of Health, Albany, NY, 12201-2002, USA.
authors.forename: April D
authors.initials: AD
authors.lastname: Davis
authors.affiliation: The Laboratory of Immunology, The Wadsworth Center, New York State Department of Health, Albany, NY, 12201-2002, USA.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Rudd
authors.affiliation: The Laboratory of Immunology, The Wadsworth Center, New York State Department of Health, Albany, NY, 12201-2002, USA.
authors.forename: Christopher T
authors.initials: CT
authors.lastname: Barron
authors.affiliation: The Department of Biomedical Sciences, The School of Public Health, The University at Albany, Albany, NY, 12201-0509, USA.
authors.forename: Cailyn
authors.initials: C
authors.lastname: Cowan
mesh_terms: D000818:Animals; D000908:Antibodies, Fungal / Q000302:isolation & purification*; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D002469:Cell Separation; D002685:Chiroptera / Q000276:immunology*; D018604:Epitope Mapping; D005434:Flow Cytometry; D007146:Immunoglobulin lambda-Chains / Q000276:immunology; D016130:Immunophenotyping; D051379:Mice; D008853:Microscopy; D009181:Mycoses / Q000276:immunology*; D015047:Zoonoses / Q000276:immunology*
chemical_list: D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal; D007146:Immunoglobulin lambda-Chains
keywords: B lymphocytes; Bats; Immunoglobulins; Microchiroptera; Monoclonal antibody