title: Chronic Omega-3 Polyunsaturated Fatty Acid Treatment Variably Affects Cellular Repolarization in a Healed Post-MI Arrhythmia Model.
abstract: BACKGROUND
  Over the last 40 years omega-3 polyunsaturated fatty acids (PUFAs) have been shown to be anti-arrhythmic or pro-arrhythmic depending on the method and duration of administration and model studied. We previously reported that omega-3 PUFAs do not confer anti-arrhythmic properties and are pro-arrhythmic in canine model of sudden cardiac death (SCD). Here, we evaluated the effects of chronic omega-3 PUFA treatment in post-MI animals susceptible (VF+) or resistant (VF-) to ventricular tachyarrhythmias.
  
  
  METHODS
  Perforated patch clamp techniques were used to measure cardiomyocyte action potential durations (APD) at 50 and 90% repolarization and short term variability of repolarization. The early repolarizing transient outward potassium current Ito was also studied.
  
  
  RESULTS
  Omega-3 PUFAs prolonged the action potential in VF- myocytes at both 50 and 90% repolarization. Short term variability of repolarization was increased in both untreated and treated VF- myocytes vs.
  
  
  METHODS
  Ito was unaffected by omega-3 PUFA treatment. Omega-3 PUFA treatment attenuated the action potential prolongation in VF+ myocytes, but did not return repolarization to control values.
  
  
  CONCLUSIONS
  Omega-3 PUFAs do not confer anti-arrhythmic properties in the setting of healed myocardial infarction in a canine model of SCD. In canines previously resistant to ventricular fibrillation (VF-), omega-3 PUFA treatment prolonged the action potential in VF- myocytes, and may contribute to pro-arrhythmic responses.
authors.affiliation: College of Pharmacy, The Ohio State UniversityColumbus, OH, USA; Department of Physiology and Cell Biology, The Ohio State UniversityColumbus, OH, USA; Dorothy M. Davis Heart and Lung Research Institute, The Ohio State UniversityColumbus, OH, USA.
authors.forename: Ingrid M
authors.initials: IM
authors.lastname: Bonilla
authors.affiliation: College of Pharmacy, The Ohio State University Columbus, OH, USA.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Nishijima
authors.affiliation: Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University Columbus, OH, USA.
authors.forename: Pedro
authors.initials: P
authors.lastname: Vargas-Pinto
authors.affiliation: College of Pharmacy, The Ohio State University Columbus, OH, USA.
authors.forename: Stephen H
authors.initials: SH
authors.lastname: Baine
authors.affiliation: College of Pharmacy, The Ohio State University Columbus, OH, USA.
authors.forename: Arun
authors.initials: A
authors.lastname: Sridhar
authors.affiliation: Division of Cardiology, Peking University People's Hospital Beijing, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Physiology and Cell Biology, The Ohio State UniversityColumbus, OH, USA; Dorothy M. Davis Heart and Lung Research Institute, The Ohio State UniversityColumbus, OH, USA.
authors.forename: George E
authors.initials: GE
authors.lastname: Billman
authors.affiliation: College of Pharmacy, The Ohio State UniversityColumbus, OH, USA; Department of Physiology and Cell Biology, The Ohio State UniversityColumbus, OH, USA; Dorothy M. Davis Heart and Lung Research Institute, The Ohio State UniversityColumbus, OH, USA.
authors.forename: Cynthia A
authors.initials: CA
authors.lastname: Carnes
keywords: electrophysiology; myocardial infarction; omega-3 polyunsaturated fatty acids; potassium current; ventricular fibrillation