title: Bee venom phospholipase A2 ameliorates motor dysfunction and modulates microglia activation in Parkinson's disease alpha-synuclein transgenic mice.
abstract: α-Synuclein (α-Syn) has a critical role in microglia-mediated neuroinflammation, which leads to the development of Parkinson's disease (PD). Recent studies have shown that bee venom (BV) has beneficial effects on PD symptoms in human patients or 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) toxin-induced PD mice. This study investigated whether treatment with BV-derived phospholipase A2 (bvPLA2) would improve the motor dysfunction and pathological features of PD in human A53T α-Syn mutant transgenic (A53T Tg) mice. The motor dysfunction of A53T Tg mice was assessed using the pole test. The levels of α-Syn, microglia and the M1/M2 phenotype in the spinal cord were evaluated by immunofluorescence. bvPLA2 treatment significantly ameliorated motor dysfunction in A53T Tg mice. In addition, bvPLA2 significantly reduced the expression of α-Syn, the activation and numbers of microglia, and the ratio of M1/M2 in A53T Tg mice. These results suggest that bvPLA2 could be a promising treatment option for PD.
authors.affiliation: Department of Physiology, College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea.
authors.forename: Minsook
authors.initials: M
authors.lastname: Ye
authors.affiliation: Korean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine (KIOM), Daegu, Republic of Korea.
authors.forename: Hwan-Suck
authors.initials: HS
authors.lastname: Chung
authors.affiliation: Department of Physiology, College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea.
authors.forename: Chanju
authors.initials: C
authors.lastname: Lee
authors.affiliation: Department of Physiology, College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea.
authors.forename: Joo
authors.initials: J
authors.lastname: Hyun Song
authors.affiliation: Acupuncture and Meridian Science Research Center, College of Korean Medical Science Graduate School, Kyung Hee University, Seoul, Republic of Korea.
authors.forename: Insop
authors.initials: I
authors.lastname: Shim
authors.affiliation: Department of Anatomy-Pointlogy, College of Korean Medicine, Gachon University, Seongnam, Republic of Korea.
authors.forename: Youn-Sub
authors.initials: YS
authors.lastname: Kim
authors.affiliation: Department of Physiology, College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea.
authors.forename: Hyunsu
authors.initials: H
authors.lastname: Bae
mesh_terms: D000818:Animals; D001514:Bee Venoms / Q000201:enzymology*; D001516:Bees; D004195:Disease Models, Animal; D057487:Enzyme Therapy; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D017628:Microglia / Q000187:drug effects / Q000473:pathology; D009043:Motor Activity / Q000187:drug effects*; D010300:Parkinson Disease / Q000188:drug therapy* / Q000235:genetics / Q000503:physiopathology*; D054467:Phospholipases A2 / Q000627:therapeutic use*; D017354:Point Mutation; D013116:Spinal Cord / Q000187:drug effects / Q000503:physiopathology; D051844:alpha-Synuclein / Q000032:analysis / Q000235:genetics
chemical_list: D001514:Bee Venoms; C497604:SNCA protein, human; D051844:alpha-Synuclein; D054467:Phospholipases A2