title: Genetic linkage map construction and QTL mapping of seedling height, basal diameter and crown width of Taxodium 'Zhongshanshan 302' × T. mucronatum.
abstract: Taxodium is a genus renowned for its fast growth, good form and tolerance of flooding, salt, alkalinity, disease and strong winds. In this study, a genetic linkage map was constructed using sequence-related amplified polymorphism (SRAP) and simple sequence repeat (SSR) markers based on an F1 population containing 148 individuals generated from a cross between T. 'Zhongshanshan 302' and T. mucronatum. The map has a total length of 976.5 cM, with a mean distance of 7.0 cM between markers, and contains 34 linkage groups with 179 markers (171 SRAPs and 8 SSRs). Quantitative trait loci (QTLs) affecting growth traits, such as seedling height, basal diameter and crown width, were detected based on the constructed linkage map. Four significant QTLs were identified, three of which, namely qtSH-1 for seedling height, qtBD-1 for basal diameter and qtCW-1 for crown width, were located at 2.659 cM of LG7 with logarithm odds values of 3.72, 3.49 and 3.93, respectively, and explained 24.9, 27.0 and 21.7 % of the total variation of the three grown traits, respectively. Another QTL for crown width (qtCW-2) was detected at 1.0 cM on LG13, with a logarithm of odds value of 3.15, and explained 31.7 % of the total variation of crown width. This is the first report on the construction of a genetic linkage map and QTL analysis in Taxodium, laying the groundwork for the construction of a high-density genetic map and QTL mapping in the genus Taxodium.
authors.affiliation: Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014 Jiangsu China.
authors.forename: Ziyang
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Key Laboratory of Forest Genetics and Gene Engineering of the Ministry of Education, Nanjing Forestry University, Nanjing, 210037 Jiangsu China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014 Jiangsu China.
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014 Jiangsu China.
authors.forename: Chaoguang
authors.initials: C
authors.lastname: Yu
authors.affiliation: Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014 Jiangsu China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014 Jiangsu China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Shi
authors.affiliation: Key Laboratory of Forest Genetics and Gene Engineering of the Ministry of Education, Nanjing Forestry University, Nanjing, 210037 Jiangsu China.
authors.forename: Ziyuan
authors.initials: Z
authors.lastname: Hao
authors.affiliation: Key Laboratory of Forest Genetics and Gene Engineering of the Ministry of Education, Nanjing Forestry University, Nanjing, 210037 Jiangsu China.
authors.forename: Huogen
authors.initials: H
authors.lastname: Li
keywords: Linkage map; QTL mapping; Sequence-related amplified polymorphism (SRAP); Simple sequence repeats (SSR); Taxodium ‘Zhongshansa’