title: Protective effects of ID331 Triticum monococcum gliadin on in vitro models of the intestinal epithelium.
abstract: A growing interest in developing new strategies for preventing coeliac disease has motivated efforts to identify cereals with null or reduced toxicity. In the current study, we investigate the biological effects of ID331 Triticum monococcum gliadin-derived peptides in human Caco-2 intestinal epithelial cells. Triticum aestivum gliadin derived peptides were employed as a positive control. The effects on epithelial permeability, zonulin release, viability, and cytoskeleton reorganization were investigated. Our findings confirmed that ID331 gliadin did not enhance permeability and did not induce zonulin release, cytotoxicity or cytoskeleton reorganization of Caco-2 cell monolayers. We also demonstrated that ID331 ω-gliadin and its derived peptide ω(105-123) exerted a protective action, mitigating the injury of Triticum aestivum gliadin on cell viability and cytoskeleton reorganization. These results may represent a new opportunity for the future development of innovative strategies to reduce gluten toxicity in the diet of patients with gluten intolerance.
authors.affiliation: Istituto di Scienze dell'Alimentazione, Consiglio Nazionale delle Ricerche, via Roma, 64, 83100 Avellino, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Iacomino
authors.affiliation: Istituto di Scienze dell'Alimentazione, Consiglio Nazionale delle Ricerche, via Roma, 64, 83100 Avellino, Italy; Dipartimento di Agraria, Università Federico II, Parco Gussone, 80055 Portici (Na), Italy.
authors.forename: Luigia
authors.initials: L
authors.lastname: Di Stasio
authors.affiliation: Istituto di Scienze dell'Alimentazione, Consiglio Nazionale delle Ricerche, via Roma, 64, 83100 Avellino, Italy.
authors.forename: Olga
authors.initials: O
authors.lastname: Fierro
authors.affiliation: Istituto di Scienze dell'Alimentazione, Consiglio Nazionale delle Ricerche, via Roma, 64, 83100 Avellino, Italy.
authors.forename: Gianluca
authors.initials: G
authors.lastname: Picariello
authors.affiliation: Istituto di Scienze dell'Alimentazione, Consiglio Nazionale delle Ricerche, via Roma, 64, 83100 Avellino, Italy.
authors.forename: Antonella
authors.initials: A
authors.lastname: Venezia
authors.affiliation: Consiglio per la Ricerca in Agricoltura e l'analisi dell'economia Agraria, Unità di Ricerca per la Valorizzazione Qualitativa dei Cereali (CRA-QCE), via Manziana, 30, 00189 Roma, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Gazza
authors.affiliation: Istituto di Scienze dell'Alimentazione, Consiglio Nazionale delle Ricerche, via Roma, 64, 83100 Avellino, Italy; Dipartimento di Agraria, Università Federico II, Parco Gussone, 80055 Portici (Na), Italy.
authors.forename: Pasquale
authors.initials: P
authors.lastname: Ferranti
authors.affiliation: Istituto di Scienze dell'Alimentazione, Consiglio Nazionale delle Ricerche, via Roma, 64, 83100 Avellino, Italy. Electronic address: mamone@isa.cnr.it.
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Mamone
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018938:Caco-2 Cells; D002446:Celiac Disease / Q000378:metabolism / Q000517:prevention & control; D005903:Gliadin / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D005983:Glutens / Q000037:antagonists & inhibitors / Q000378:metabolism; D006801:Humans; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000378:metabolism; D010455:Peptides / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D010539:Permeability; D013552:Swine; D014908:Triticum / Q000235:genetics / Q000378:metabolism
chemical_list: D010455:Peptides; D005983:Glutens; D005903:Gliadin
keywords: Caco-2; Coeliac disease; Cytotoxicity; F-actin; Triticum monococcum; Zonulin; ω-Gliadin