title: The Circular RNA Cdr1as Act as an Oncogene in Hepatocellular Carcinoma through Targeting miR-7 Expression.
abstract: CircRNAs are a class of endogenous RNA that regulates gene expression at the post-transcriptional or transcriptionallevel through interacting with other molecules or microRNAs. Increasing studies have demonstrated that circRNAs play a crucial role in biology processes. CircRNAs are proved as potentialbiomarkers in many diseases including cancers. However, the role of Cdr1as in Hepatocellular carcinoma (HCC) remains to be elucidated. We demonstrated that Cdr1as expression was upregulated in HCC tissues compared with the adjacent non-tumor tissues. In addtion, miR-7 expression was downregulated in HCC tissues compared with the adjacent non-tumor tissues. Moreover, the expression level of miR-7 was inversely correlated with that in HCC tissues. Knockdown of Cdr1as suppressed the HCC cell proliferation and invasion. Overexpression of miR-7 inhibited the HCC cell proliferation and invasion. Overexpression of miR-7 could suppress the direct target gene CCNE1 and PIK3CD expression. Knockdown of Cdr1as suppressed the expression of miR-7 and also inhibited the CCNE1 and PIK3CD expression. Furthermore, knockdown of Cdr1as suppressed the HCC cell proliferation and invasion through targeting miR-7. These data suggested that Cdr1as acted as an oncogene partly through targeting miR-7 in HCC.
authors.affiliation: Department of Infectious Disease,The Fourth Hospital of Harbin Medical University, Harbin 150001, Heilongjiang, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yu
authors.affiliation: Department of Biliary and Pancreatic Surgery, Xiangya Hospital of Central South University, Changsha, 410008, Hunan, China.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Gong
authors.affiliation: Department of Ophthalmology,The Fourth Hospital of Harbin Medical University, Harbin 150001, Heilongjiang, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Sun
authors.affiliation: College of Computer Science and Technology (Network and Information Security), JiLin University, Changchun, 130001, Jilin, China.
authors.forename: Qiying
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Department of Infectious Disease,The Fourth Hospital of Harbin Medical University, Harbin 150001, Heilongjiang, China.
authors.forename: Baoling
authors.initials: B
authors.lastname: Lu
authors.affiliation: Department of Infectious Disease,The Fourth Hospital of Harbin Medical University, Harbin 150001, Heilongjiang, China.
authors.forename: Liying
authors.initials: L
authors.lastname: Zhu
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D049109:Cell Proliferation / Q000235:genetics; D058534:Class I Phosphatidylinositol 3-Kinases / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics; D009857:Oncogenes; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D058534:Class I Phosphatidylinositol 3-Kinases; D035683:MicroRNAs; D009363:Neoplasm Proteins; D062085:RNA, Long Noncoding; D012334:RNA, Neoplasm; C549242:MIRN7-1 microRNA, human; C000613369:long non-coding RNA CDR1AS, human; C486018:PIK3CD protein, human