title: Simple Method for Detecting Glycoproteins Dot-blotted or Electro-blotted on to a Polyvinylidene Difluoride Membrane.
abstract: A simple method is described for detecting glycoproteins which had been dot-blotted or electro-blotted on to a polyvinylidene difluoride (PVDF) membrane after sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. This method is based on the periodate oxidation of glycoproteins on a PVDF membrane with subsequent staining by the chromophoric hydrazide, 4-N,N-dimethylamino-4'-azobenzene sulfonyl hydrazide. The glycoproteins could be visualized as red-colored spots or bands in the range of 0.16-0.31 μγ.
authors.affiliation: a Department of Biological Resource Sciences, Graduate School of Agriculture, Tohoku University.
authors.forename: S
authors.initials: S
authors.lastname: Kabuto
authors.affiliation: a Department of Biological Resource Sciences, Graduate School of Agriculture, Tohoku University.
authors.forename: K
authors.initials: K
authors.lastname: Muramoto
keywords: PVDF membrane; blotting; glycoprotein; hydrazide; sugar