title: Antibacterial Activity of Hibicuslide C on Multidrug-Resistant Pseudomonas aeruginosa Isolates.
abstract: Pseudomonas aeruginosa is a gram-negative bacterium that is frequently related to natural resistance to many drugs. In this work, the inhibition of growth against P. aeruginosa and multidrug-resistant P. aeruginosa (MDRPA) isolated from patients at Kyungpook National University was confirmed for hibicuslide C, essential oil components from Abutilon theophrasti. Hibicuslide C has antifungal activity with membrane disruption and apoptotic response against Candida albicans. However, its antibacterial activity was not reported yet. Cells treated with hibicuslide C was showed that its antipseudomonal activity is related to gDNA fragmentation and damage by TUNEL and gDNA electrophoresis. Furthermore, hibicuslide C worked synergistically with fluoroquinolones and rifampicin against MDRPA regardless of the ATP-associated mechanism. The antibiofilm activity possessed sole-resulting tissue culture plate method; besides that, the antibiofilm activity of other antibiotics was supported in particular MDRPA. The essential oil components like hibicuslide C may have antipseudomonal activity and, furthermore, increase in bacterial antibiotic susceptibility.
authors.affiliation: BK 21 Plus KNU Creative BioResearch Group, School of Life Sciences, College of Natural Sciences, Kyungpook National University, Daehak-ro 80, Buk-gu, Daegu, 702-701, Republic of Korea.
authors.forename: Heejeong
authors.initials: H
authors.lastname: Lee
authors.affiliation: BK 21 Plus KNU Creative BioResearch Group, School of Life Sciences, College of Natural Sciences, Kyungpook National University, Daehak-ro 80, Buk-gu, Daegu, 702-701, Republic of Korea.
authors.forename: Hyemin
authors.initials: H
authors.lastname: Choi
authors.affiliation: Department of Microbiology, Kyungpook National University School of Medicine, Daegu, 700-422, Republic of Korea.
authors.forename: Je Chul
authors.initials: JC
authors.lastname: Lee
authors.affiliation: Department of Microbiology, Kyungpook National University School of Medicine, Daegu, 700-422, Republic of Korea.
authors.forename: Yoo Chul
authors.initials: YC
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Chosun University, 375 Seosuk-dong, Dong-gu, Gwangju, 501-759, Republic of Korea.
authors.forename: Eun-Rhan
authors.initials: ER
authors.lastname: Woo
authors.affiliation: BK 21 Plus KNU Creative BioResearch Group, School of Life Sciences, College of Natural Sciences, Kyungpook National University, Daehak-ro 80, Buk-gu, Daegu, 702-701, Republic of Korea. dglee222@knu.ac.kr.
authors.forename: Dong Gun
authors.initials: DG
authors.lastname: Lee
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects; D024901:Drug Resistance, Multiple, Bacterial; D006801:Humans; D019684:Magnoliopsida / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D009822:Oils, Volatile / Q000494:pharmacology*; D010666:Phenylpropionates / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents; D009822:Oils, Volatile; D010666:Phenylpropionates; D010936:Plant Extracts; C000595664:hibicuslide C