title: Evaluation of a High-Intensity Green Fluorescent Protein Fluorophage Method for Drug- Resistance Diagnosis in Tuberculosis for Isoniazid, Rifampin, and Streptomycin.
abstract: A novel method for detecting drug resistance in Mycobacterium tuberculosis using mycobacteriophage Φ (2) GFP10 was evaluated with clinical isolates. The phage facilitates microscopic fluorescence detection due to the high expression of green fluorescence protein which also simplifies the operative protocol as well. A total of 128 clinical isolates were tested by the phage assay for isoniazid (INH), rifampin (RIF), and streptomycin (STR) resistance while conventional drug susceptibility test, by MGIT960, was used as reference. The sensitivities of Φ (2) GFP10 assay for INH, RIF, and STR resistance detection were 100, 98.2, and 89.3%, respectively while their specificities were 85.1, 98.6, and 95.8%, respectively. The agreement between phage and conventional assay for detecting INH, RIF, and STR resistance was 92.2, 98.4, and 93.0%, respectively. The Φ (2) GFP10-phage results could be available in 2 days for RIF and STR, while it takes 3 days for INH, with an estimated cost of less than $2 to test all the three antibiotics. The Φ (2) GFP10-phage method has the potential to be a valuable, rapid and economical screening method for detecting drug-resistant tuberculosis.
authors.affiliation: National Clinical Laboratory on Tuberculosis, Beijing Key Laboratory for Drug-Resistant Tuberculosis Research, Beijing Chest Hospital, Beijing Tuberculosis and Thoracic Tumor Institute, Capital Medical University Beijing, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Yu
authors.affiliation: National Clinical Laboratory on Tuberculosis, Beijing Key Laboratory for Drug-Resistant Tuberculosis Research, Beijing Chest Hospital, Beijing Tuberculosis and Thoracic Tumor Institute, Capital Medical University Beijing, China.
authors.forename: Yunting
authors.initials: Y
authors.lastname: Gu
authors.affiliation: National Clinical Laboratory on Tuberculosis, Beijing Key Laboratory for Drug-Resistant Tuberculosis Research, Beijing Chest Hospital, Beijing Tuberculosis and Thoracic Tumor Institute, Capital Medical University Beijing, China.
authors.forename: Guanglu
authors.initials: G
authors.lastname: Jiang
authors.affiliation: National Clinical Laboratory on Tuberculosis, Beijing Key Laboratory for Drug-Resistant Tuberculosis Research, Beijing Chest Hospital, Beijing Tuberculosis and Thoracic Tumor Institute, Capital Medical University Beijing, China.
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Ma
authors.affiliation: National Clinical Laboratory on Tuberculosis, Beijing Key Laboratory for Drug-Resistant Tuberculosis Research, Beijing Chest Hospital, Beijing Tuberculosis and Thoracic Tumor Institute, Capital Medical University Beijing, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Zhao
authors.affiliation: National Clinical Laboratory on Tuberculosis, Beijing Key Laboratory for Drug-Resistant Tuberculosis Research, Beijing Chest Hospital, Beijing Tuberculosis and Thoracic Tumor Institute, Capital Medical University Beijing, China.
authors.forename: Zhaogang
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Department of Microbiology and Immunology, Albert Einstein College of Medicine Bronx, NY, USA.
authors.forename: Paras
authors.initials: P
authors.lastname: Jain
authors.affiliation: Division of Pulmonary, Allergy, and Critical Care Medicine, Columbia University Medical CenterNew York, NY, USA; Department of Epidemiology, Mailman School of Public Health, Columbia University Medical CenterNew York, NY, USA.
authors.forename: Max
authors.initials: M
authors.lastname: O'Donnell
authors.affiliation: Department of Microbiology and Immunology, Albert Einstein College of Medicine Bronx, NY, USA.
authors.forename: Michelle
authors.initials: M
authors.lastname: Larsen
authors.affiliation: Department of Microbiology and Immunology, Albert Einstein College of MedicineBronx, NY, USA; Howard Hughes Medical InstituteChevy Chase, MD, USA.
authors.forename: William R
authors.initials: WR
authors.lastname: Jacobs
authors.affiliation: National Clinical Laboratory on Tuberculosis, Beijing Key Laboratory for Drug-Resistant Tuberculosis Research, Beijing Chest Hospital, Beijing Tuberculosis and Thoracic Tumor Institute, Capital Medical University Beijing, China.
authors.forename: Hairong
authors.initials: H
authors.lastname: Huang
keywords: diagnosis; drug resistance; mycobacteriophage; tuberculosis; Φ2GFP10