title: Comparison of the Antifungal Efficacy of 1.3% NaOCl/MTAD with Other Routine Irrigants: An Ex-Vivo Study.
abstract: Objectives. To evaluate and compare the antifungal efficacy of 1.3% NaOCl/MTAD with 2.5% sodium hypochlorite (NaOCl), 2% chlorhexidine gluconate (CHX), and iodine potassium iodide (IKI). Materials and Methods. Fifty-two single rooted teeth were used which were divided into four groups with 10 teeth in each group: 2.5% NaOCl, 2% CHX, IKI, 1.3% NaOCl/MTAD, and physiologic saline. Two teeth served as negative controls and were placed in fresh brain-heart infusion broth (BHI) after autoclaving. The teeth were inoculated and incubated with Candida albicans after which the teeth were instrumented and irrigated with the test irrigants. The first microbial sampling was then performed and colony forming unit/mL (cfu/mL) was counted. The second microbial sampling was performed 1 week after instrumentation and irrigation. Results. The test irrigants were effective against C. albicans in both the first and second microbial samplings. When the irrigants were compared, there was no statistical difference in their activity in the 1st and 2nd microbial sampling. On comparison of the change in mean cfu/mL between the 1st and 2nd microbial samplings, the antifungal activity of the test irrigants was in the order 2.5% NaOCl > 2% CHX > 1.3% NaOCl/MTAD > IKI.
authors.affiliation: Conservative Dentistry & Endodontics, Manav Rachna Dental College, Manav Rachna Educational Institutions Aravalli Campus, Sector 43, Delhi-Surajkund Road, Faridabad, Haryana 121004, India.
authors.forename: Neha
authors.initials: N
authors.lastname: Juneja
authors.affiliation: Conservative Dentistry & Endodontics, Department of Conservative Dentistry & Endodontics, A. B. Shetty Memorial Institute of Dental Sciences, Medical Sciences Complex, Deralakatte, Mangalore, Karnataka 575018, India.
authors.forename: Mithra N
authors.initials: MN
authors.lastname: Hegde