title: Effective Cloning of Unmarked DNA Fragments in the Bacillus subtilis 168 Genome.
abstract: The Bacillus subtilis 168 genome is being used to clone DNA segments, particularly unmarked DNA segments between the two available clones. To facilitate this cloning, a counter selection method was developed in which loss of a cI repressor gene rendered the host strain resistant to neomycin. This method is promoted to use the B. subtilis genome as a general cloning vehicle.
authors.affiliation: a Mitsubishi Kasei Institute of Life Sciences.
authors.forename: M
authors.initials: M
authors.lastname: Itaya
keywords: inducible promoter; positive selection; repressor binding