title: Effects of combination of melatonin and laser irradiation on ovarian cancer cells and endothelial lineage viability.
abstract: The main goal of anti-cancer therapeutic approaches is to induce apoptosis in tumor masses but not in the normal tissues. Nevertheless, the combination of photodynamic irradiation with complementary oncostatic agents contributes to better therapeutic performance. Here, we applied two different cell lines; SKOV3 ovarian carcinoma cells and HUVECs umbilical cord cells as in vitro models to pinpoint whether pharmacological concentration of melatonin in combination with photodynamic therapy induces cell cytotoxicity. The cells were separately treated with various concentrations of melatonin (0 to 10 mM) and photodynamic irradiation alone or in combination. Cells were preliminary exposed to increasing concentrations of melatonin for 24 h and subsequently underwent laser irradiation for 60 s with an output power of 80 mW in continuous mode at 675 nm wavelength and a total light dose of 13.22 J/cm2. Cell viability, apoptosis/necrosis rates, and reactive oxygen species levels as well as heat shock protein 70 expression were monitored after single and combined treatments. A statistical analysis was performed by applying one-way analysis of variance (ANOVA) and post hoc Tukey's test. Combination treatment of both cell lines caused a marked increase in apoptosis/necrosis rate, reactive oxygen species generation, and heat shock protein 70 expression compared to incubation of the cells with each agent alone (p < 0.05). SKOV3 cancer cells expressed higher level of heat shock protein 70 under experimental procedure as compared to HUVECs (p < 0.05). Our results introduce melatonin as a potent stimulus for enhancing the efficacy of laser on induction of apoptosis in tumor cells.
authors.affiliation: Stem Cell Research Center, Tabriz University of Medical Sciences, Imam Reza St., Golgasht St., 5166614756, Tabriz, Iran.|Department of Clinical Biochemistry and Laboratory Medicine, Tabriz University of Medical Sciences, Imam Reza St., Golgasht St., 5166614756, Tabriz, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Akbarzadeh
authors.affiliation: Stem Cell Research Center, Tabriz University of Medical Sciences, Imam Reza St., Golgasht St., 5166614756, Tabriz, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Nouri
authors.affiliation: Stem Cell Research Center, Tabriz University of Medical Sciences, Imam Reza St., Golgasht St., 5166614756, Tabriz, Iran.
authors.forename: Maryam Vahidi
authors.initials: MV
authors.lastname: Banekohal
authors.affiliation: Stem Cell Research Center, Tabriz University of Medical Sciences, Imam Reza St., Golgasht St., 5166614756, Tabriz, Iran.|Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
authors.forename: Omid
authors.initials: O
authors.lastname: Cheraghi
authors.affiliation: Research Institute for Applied Physics and Astronomy, University of Tabriz, Tabriz, Iran.
authors.forename: Habib
authors.initials: H
authors.lastname: Tajalli
authors.affiliation: Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Aliakbar
authors.initials: A
authors.lastname: Movassaghpour
authors.affiliation: Stem Cell Research Center, Tabriz University of Medical Sciences, Imam Reza St., Golgasht St., 5166614756, Tabriz, Iran.
authors.forename: Sina
authors.initials: S
authors.lastname: Soltani
authors.affiliation: Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
authors.forename: Hadi
authors.initials: H
authors.lastname: Cheraghi
authors.affiliation: Stem Cell Research Center, Tabriz University of Medical Sciences, Imam Reza St., Golgasht St., 5166614756, Tabriz, Iran.|Department of Clinical Biochemistry and Laboratory Medicine, Tabriz University of Medical Sciences, Imam Reza St., Golgasht St., 5166614756, Tabriz, Iran.
authors.forename: Navid
authors.initials: N
authors.lastname: Feizy
authors.affiliation: Stem Cell Research Center, Tabriz University of Medical Sciences, Imam Reza St., Golgasht St., 5166614756, Tabriz, Iran.
authors.forename: Soheila
authors.initials: S
authors.lastname: Montazersaheb
authors.affiliation: Stem Cell Research Center, Tabriz University of Medical Sciences, Imam Reza St., Golgasht St., 5166614756, Tabriz, Iran. Rezarahbardvm@gmail.com.
authors.forename: Reza
authors.initials: R
authors.lastname: Rahbarghazi
authors.affiliation: Stem Cell Research Center, Tabriz University of Medical Sciences, Imam Reza St., Golgasht St., 5166614756, Tabriz, Iran. drnsamadi@yahoo.com.|Department of Clinical Biochemistry and Laboratory Medicine, Tabriz University of Medical Sciences, Imam Reza St., Golgasht St., 5166614756, Tabriz, Iran. drnsamadi@yahoo.com.
authors.forename: Nasser
authors.initials: N
authors.lastname: Samadi
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D006801:Humans; D008550:Melatonin / Q000494:pharmacology*; D010051:Ovarian Neoplasms / Q000188:drug therapy*; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D018840:HSP70 Heat-Shock Proteins; D017319:Photosensitizing Agents; D017382:Reactive Oxygen Species; D008550:Melatonin
keywords: HUVECs; Heat shock protein 70; Laser irradiation; Melatonin; SKOV3