title: Characterization of quality of sediments from Paranaguá Bay (Brazil) by combined in vitro bioassays and chemical analyses.
abstract: The present study characterizes the quality of sediments from the Paranaguá Estuarine Complex (South Brazil). Polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), and organochlorine pesticides (OCPs) were determined in sediment samples together with a series of different in vitro bioassays. The fish hepatoma cell line (PLHC-1) was used to determine the presence of cytotoxic compounds and CYP1A- and oxidative stress-inducing agents in sediment extracts. Ovarian microsomal fractions from sea bass (Dicentrarchus labrax) were used to detect the presence of endocrine disrupters that interfered with the synthesis of estrogens (ovarian CYP19). Despite the relatively low levels of pollutants and no evidence of negative effects based on guideline levels, sediments collected close to harbors were enriched with CYP1A-inducing agents and they showed higher cytotoxicity. In contrast, sediments from internal areas inhibited CYP19 activity, which suggests the presence of endocrine disrupters at these sites. Overall, the selected bioassays and the chemistry data led to the identification of potentially impacted areas along the Paranaguá Estuarine Complex that would require further action to improve their environmental quality. Environ Toxicol Chem 2017;36:1811-1819. © 2016 SETAC.
authors.affiliation: Water Resources and Environmental Engineering Graduate Program, Federal University of Paraná, Curitiba, Paraná, Brazil.|Department of Environmental Chemistry, Institute of Environmental Assessment and Water Research, Spanish Council for Scientific Research, Barcelona, Catalunya, Spain.
authors.forename: Juliane
authors.initials: J
authors.lastname: Rizzi
authors.affiliation: Department of Environmental Chemistry, Institute of Environmental Assessment and Water Research, Spanish Council for Scientific Research, Barcelona, Catalunya, Spain.
authors.forename: Elisabet
authors.initials: E
authors.lastname: Pérez-Albaladejo
authors.affiliation: Department of Environmental Chemistry, Institute of Environmental Assessment and Water Research, Spanish Council for Scientific Research, Barcelona, Catalunya, Spain.
authors.forename: Denise
authors.initials: D
authors.lastname: Fernandes
authors.affiliation: Department of Environmental Chemistry, Institute of Environmental Assessment and Water Research, Spanish Council for Scientific Research, Barcelona, Catalunya, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Contreras
authors.affiliation: Water Resources and Environmental Engineering Graduate Program, Federal University of Paraná, Curitiba, Paraná, Brazil.|Department of Environmental Engineering, Federal University of Paraná, Curitiba, Paraná, Brazil.
authors.forename: Sandro
authors.initials: S
authors.lastname: Froehner
authors.affiliation: Department of Environmental Chemistry, Institute of Environmental Assessment and Water Research, Spanish Council for Scientific Research, Barcelona, Catalunya, Spain.
authors.forename: Cinta
authors.initials: C
authors.lastname: Porte
mesh_terms: D000818:Animals; D001141:Aromatase / Q000378:metabolism; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism; D001492:Bass / Q000378:metabolism; D061006:Bays; D001681:Biological Assay; D001938:Brazil; D002460:Cell Line; D052244:Endocrine Disruptors / Q000737:chemistry / Q000633:toxicity; D004784:Environmental Monitoring; D004967:Estrogens / Q000096:biosynthesis; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D019015:Geologic Sediments / Q000032:analysis* / Q000737:chemistry; D008861:Microsomes / Q000187:drug effects / Q000201:enzymology; D010053:Ovary / Q000378:metabolism; D011078:Polychlorinated Biphenyls / Q000032:analysis / Q000737:chemistry; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000737:chemistry; D025341:Principal Component Analysis; D017382:Reactive Oxygen Species / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000737:chemistry / Q000633:toxicity
chemical_list: D052244:Endocrine Disruptors; D004967:Estrogens; D011084:Polycyclic Aromatic Hydrocarbons; D017382:Reactive Oxygen Species; D014874:Water Pollutants, Chemical; D011078:Polychlorinated Biphenyls; D001141:Aromatase; D001189:Aryl Hydrocarbon Hydroxylases
keywords: Biomonitoring; Endocrine disruption; In vitro bioassays; Organic pollutants; Sediment contamination