title: [The preventive effect of total saponins of Panax japonicus on nonsteroidal anti-inflammatory drug-induced enteropathy].
abstract: Objective To investigate the preventive effect of total saponins of Panax japonicus (SPJ) on nonsteroidal anti-inflammatory drug (NSAID)-induced intestinal injuries in mice. Methods NSAID-induced intestinal mucosal damaged models were established by intragastric administration of 5 mg/mL diclofenac sodium in mice for continuous 2 days. Since 4 days before the modeling, the mice in the experimental group were given SPJ once a day for 6 days. The small intestinal mucosal permeability was detected by fluorescein isothiocyanate-dextran (FITC-DT) and Evans blue staining. The small intestinal mucosal lesions were observed by HE staining; immunofluorescence staining was used to determine the expressions of endoplasmic reticulum stress response protein glucose transporter protein 78 (GRP78) and tumor necrosis factor alpha (TNF-α) in the intestinal mucosal epithelial cells. Results In the model group, small intestinal mucosal injury was serious. Evans blue staining and FITC-DT labeling showed the blue spots were denser and mucosal permeability increased significantly in the model group. HE staining revealed the intestinal mucosal villus degeneration, necrosis, shedding, and inflammatory cell infiltration in the model group, which meant the intestinal mucosal damage models were successfully established. And different concentrations of SPJ protected intestinal mucosa, meanwhile the expressions of GRP78 and TNF- α were significantly reduced in the intestinal epithelial cells as indicated by immunofluorescence staining. Conclusion There is a certain preventive effect of total SPJ on NSAID-induced intestinal injuries via the endoplasmic reticulum stress pathway.
authors.affiliation: Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, Three Gorges University, Yichang 443002, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Mei
authors.affiliation: Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, Three Gorges University, Yichang 443002, China.
authors.forename: Zhaozhao
authors.initials: Z
authors.lastname: Xiao
authors.affiliation: Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, Three Gorges University, Yichang 443002, China.
authors.forename: Jinwu
authors.initials: J
authors.lastname: Feng
authors.affiliation: Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, Three Gorges University, Yichang 443002, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.affiliation: Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, Three Gorges University, Yichang 443002, China.
authors.forename: Changcheng
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, Three Gorges University, Yichang 443002, China.
authors.forename: Ding
authors.initials: D
authors.lastname: Yuan
authors.affiliation: Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, Three Gorges University, Yichang 443002, China. *Corresponding author, E-mail: zqliu@ctgu.edu.cn.
authors.forename: Chaoqi
authors.initials: C
authors.lastname: Liu
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000633:toxicity*; D004008:Diclofenac / Q000633:toxicity; D000091342:Endoplasmic Reticulum Chaperone BiP; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects; D006360:Heat-Shock Proteins / Q000378:metabolism; D007410:Intestinal Diseases / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007421:Intestine, Small / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D005894:Panax / Q000737:chemistry*; D008517:Phytotherapy; D012503:Saponins / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000091342:Endoplasmic Reticulum Chaperone BiP; D006360:Heat-Shock Proteins; C000717248:Hspa5 protein, mouse; D012503:Saponins; D014409:Tumor Necrosis Factor-alpha; D004008:Diclofenac