title: Toxicity of Bacillus thuringiensis crystal proteins against eri silkworm, Samia cynthia ricini (Lepidoptera: Saturniidae).
abstract: Ten purified crystal proteins of Bacillus thuringiensis (Bt) were tested at concentrations ranging from 2.93 to 3000ng/cm(2) for their toxicity to eri silkworm through protein paint bioassays using castor leaves. Based on LC50 values, Cry1Aa (2.6ng/cm(2)) was highly toxic followed by Cry1Ac (29.3ng/cm(2)) and Cry1Ab (68.7ng/cm(2)). The Cry1Ca and Cry1Ea proteins were moderately toxic to eri silkworm larvae and resulted in 23% and 28% mortality, respectively at the highest concentration tested (3000ng/cm(2)). Only reduction in larval weight was observed with Cry2Aa, Cry1Da and Cry9Aa proteins while Cry3Aa and Cry1Ba proteins were found to be nontoxic.
authors.affiliation: ICAR- Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad 500 030, India; Department of Genetics, Osmania University, Hyderabad 500 030, India.
authors.forename: Donthula
authors.initials: D
authors.lastname: Sandeep Kumar
authors.affiliation: ICAR- Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad 500 030, India; Department of Genetics, Osmania University, Hyderabad 500 030, India.
authors.forename: Muddanuru
authors.initials: M
authors.lastname: Tarakeswari
authors.affiliation: ICAR- Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad 500 030, India.
authors.forename: Maddukuri
authors.initials: M
authors.lastname: Lakshminarayana
authors.affiliation: ICAR- Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad 500 030, India. Electronic address: sujatador@gmail.com.
authors.forename: Mulpuri
authors.initials: M
authors.lastname: Sujatha
mesh_terms: D000818:Animals; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000633:toxicity*; D012831:Bombyx; D004731:Endotoxins / Q000633:toxicity*; D006460:Hemolysin Proteins / Q000633:toxicity*; D007306:Insecticides; D010572:Pest Control, Biological / Q000379:methods*
chemical_list: D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; C030956:insecticidal crystal protein, Bacillus Thuringiensis
keywords: Bacillus thuringiensis; Crystal proteins; Eri silkworm; Insect bioassays; Samia ricini