title: The efficacy of intravitreal interferon alpha-2b for the treatment of experimental endotoxin-induced uveitis.
abstract: OBJECTIVE
  To study the efficacy of intravitreal interferon alpha-2b for endotoxin-induced uveitis.
  
  
  METHODS
  A total of 36 rabbits were randomly allocated to one of the three groups: (1) received interferon plus balanced-salt solution; (2) received lipopolysaccharide (LPS) plus interferon; and (3) received LPS plus balanced-salt solution. Intraocular inflammation was evaluated by slit-lamp biomicroscopy (standardization of uveitis nomenclature grading), binocular indirect ophthalmoscopy (BIO) score, and histopathology.
  
  
  RESULTS
  Group 2 showed significantly lower mean (±standard deviation) anterior chamber reaction than Group 3 (3.1 ± 0.9 vs. 3.8 ± 0.4) on day 1 postinjection, lower vitreous cells on days 1 through 7 (day 1: 3.1 ± 0.9 vs. 3.8 ± 0.4; day 3: 2.1 ± 1.6 vs. 3.8 ± 0.4; day 7: 1.9 ± 1.3 vs. 3.6 ± 0.7), and lower BIO score on days 1-7 (day 1: 3.3 ± 1.2 vs. 4.4 ± 0.7; day 3: 3.0 ± 1.4 vs. 4.3 ± 0.9; day 7: 2.4 ± 1.4 vs. 3.7 ± 1.2). The protein content of anterior and vitreous aspirates was lower in Group 2 than 3 (1618.5 ± 411.4 vs. 2567.3 ± 330.8 and 2157.0 ± 283.3 vs. 3204.6 ± 259.5, respectively).
  
  
  CONCLUSIONS
  Intravitreal interferon alpha-2b was effective in controlling endotoxin-induced uveitis.
authors.affiliation: Department of Ophthalmology, Poostchi Eye Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Afarid
authors.affiliation: Department of Ophthalmology, Poostchi Eye Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Lashkarizadeh
authors.affiliation: Department of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Mohammad J
authors.initials: MJ
authors.lastname: Ashraf
authors.affiliation: Department of Ophthalmology, Poostchi Eye Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Nowroozzadeh
authors.affiliation: Department of Biochemistry, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Sayed M
authors.initials: SM
authors.lastname: Shafiee
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004731:Endotoxins / Q000633:toxicity; D007155:Immunologic Factors / Q000008:administration & dosage; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000008:administration & dosage*; D058449:Intravitreal Injections; D033401:Microscopy, Acoustic; D009887:Ophthalmoscopy; D011817:Rabbits; D011994:Recombinant Proteins / Q000008:administration & dosage; D014605:Uveitis / Q000139:chemically induced / Q000175:diagnosis / Q000188:drug therapy*
chemical_list: D004731:Endotoxins; D007155:Immunologic Factors; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011994:Recombinant Proteins