title: Quantification of Membrane Protein Dynamics and Interactions in Plant Cells by Fluorescence Correlation Spectroscopy.
abstract: Deciphering the dynamics of protein and lipid molecules on appropriate spatial and temporal scales may shed light on protein function and membrane organization. However, traditional bulk approaches cannot unambiguously quantify the extremely diverse mobility and interactions of proteins in living cells. Fluorescence correlation spectroscopy (FCS) is a powerful technique to describe events that occur at the single-molecule level and on the nanosecond to second timescales; therefore, FCS can provide data on the heterogeneous organization of membrane systems. FCS can also be combined with other microscopy techniques, such as super-resolution techniques. More importantly, FCS is minimally invasive, which makes it an ideal approach to detect the heterogeneous distribution and dynamics of key proteins during development. In this review, we give a brief introduction about the development of FCS and summarize the significant contributions of FCS in understanding the organization of plant cell membranes and the dynamics and interactions of membrane proteins. We also discuss the potential applications of this technique in plant biology.
authors.affiliation: College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; University of Chinese Academy of Sciences, Beijing 10049, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Xing
authors.affiliation: College of Life Sciences, Henan Normal University, Xinxiang 453007, China.
authors.forename: Zongbo
authors.initials: Z
authors.lastname: Qiu
authors.affiliation: The Health Science Center, Peking University, Beijing 100191, China.
authors.forename: Qihua
authors.initials: Q
authors.lastname: He
authors.affiliation: College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China. Electronic address: linjx@ibcas.ac.cn.
authors.forename: Jinxing
authors.initials: J
authors.lastname: Lin
mesh_terms: D008565:Membrane Proteins / Q000378:metabolism*; D059828:Plant Cells / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D008565:Membrane Proteins; D010940:Plant Proteins
keywords: dynamics; fluorescence correlation spectroscopy; interaction; membrane protein; microdomain