title: Limits of Peripheral Blood Mononuclear Cells for Gene Expression-Based Biomarkers in Juvenile Idiopathic Arthritis.
abstract: Juvenile Idiopathic Arthritis (JIA) is one of the most common chronic disease conditions affecting children in the USA. As with many rheumatic diseases, there is growing interest in using genomic technologies to develop biomarkers for either diagnosis or to guide treatment ("personalized medicine"). Here, we explore the use of gene expression patterns in peripheral blood mononuclear cells (PBMC) as a first step approach to developing such biomarkers. Although PBMC carry many theoretical advantages for translational research, we have found that sample heterogeneity makes RNASeq on PBMC unsuitable as a first-step method for screening biomarker candidates in JIA. RNASeq studies of homogeneous cell populations are more likely to be useful and informative.
authors.affiliation: Department of Pediatrics, University at Buffalo, Buffalo, NY, USA.
authors.forename: Laiping
authors.initials: L
authors.lastname: Wong
authors.affiliation: Department of Pediatrics, University at Buffalo, Buffalo, NY, USA.
authors.forename: Kaiyu
authors.initials: K
authors.lastname: Jiang
authors.affiliation: Department of Pediatrics, University at Buffalo, Buffalo, NY, USA.
authors.forename: Yanmin
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Pediatrics, University at Buffalo, Buffalo, NY, USA.
authors.forename: Teresa
authors.initials: T
authors.lastname: Hennon
authors.affiliation: Department of Pediatrics, University at Buffalo, Buffalo, NY, USA.
authors.forename: Lucy
authors.initials: L
authors.lastname: Holmes
authors.affiliation: Department of Pediatrics, University of Washington, Seattle, WA, USA.
authors.forename: Carol A
authors.initials: CA
authors.lastname: Wallace
authors.affiliation: Department of Pediatrics, University at Buffalo, Buffalo, NY, USA.|Genetics, Genomics, &Bioinformatics Program, University at Buffalo, Buffalo, NY, USA.
authors.forename: James N
authors.initials: JN
authors.lastname: Jarvis
mesh_terms: D000293:Adolescent; D001171:Arthritis, Juvenile / Q000097:blood / Q000175:diagnosis / Q000235:genetics*; D015415:Biomarkers / Q000378:metabolism*; D002648:Child; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D018740:Genetic Heterogeneity; D005819:Genetic Markers; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry; D008297:Male; D015203:Reproducibility of Results; D017423:Sequence Analysis, RNA / Q000379:methods
chemical_list: D015415:Biomarkers; D005819:Genetic Markers