title: Helicase Assays.
abstract: Helicases are a class of enzymes which are motor proteins using energy derived from ATP hydrolysis to move directionally along a nucliec acid phosphodiester backbone (such as DNA, RNA and DNA-RNA hybrids) and separate two annealed nucleic acid strands. Many cellular processes, such as transcription, DNA replication, recombination and DNA repair involve helicase activity. Here, we provide a protocol to analyze helicase activitiesin vitro. In this protocol, the DNA helicase protein Merkel cell polyomavirus large T-antigen was expressed in the mammalian cell line HEK293 and immoblized on an IgG resin. The helicase assay is performing while the protein is immoblized on IgG resin.
authors.affiliation: Depatment of Molecular Genetics, Cleveland Clinic Lerner Research Institute, Cleveland, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Tumor Microenvironment and Metastasis Program, The Wistar Institute Cancer Center, Philadelphia, USA.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Microbiology, University of Pennsylvania, Philadelphia, USA.
authors.forename: Jason
authors.initials: J
authors.lastname: Diaz
authors.affiliation: Department of Microbiology, University of Pennsylvania, Philadelphia, USA.
authors.forename: Jianxin
authors.initials: J
authors.lastname: You