title: Bioaugmentation-Assisted Phytostabilisation of Abandoned Mine Sites in South West Sardinia.
abstract: Bioaugmentation-assisted phytoremediation implies the administration of selected plant growth promoting bacteria, which significantly improve plant growth and sequestration of heavy metals. In this work, 184 bacterial strains associated with roots of Pistacia lentiscus were isolated from plants spontaneously growing in the abandoned Sardinian mining areas (SW Sardinia, Italy) and phylogenetically characterised. Twenty-one bacterial isolates were assayed for properties relevant for plant growth promotion and metal tolerance. Five different strains, belonging to the genera Novosphingobium, Variovorax, Streptomyces, Amycolatopsis, Pseudomonas, were selected based on their properties for the greenhouse phytoremediation tests. Among the tested inocula, the strain Variovorax sp. RA128A, able to produce ACC deaminase and siderophore, was able to significantly enhance germination and increase length and weight of shoots and roots. Irrespective of the applied treatment, mastic shrub was able to accumulate Cd, Pb and Zn especially in roots.
authors.affiliation: Department of Biomedical Sciences, University of Cagliari, Monserrato, Cagliari, Italy. etamburini@unica.it.
authors.forename: E
authors.initials: E
authors.lastname: Tamburini
authors.affiliation: Department of Biomedical Sciences, University of Cagliari, Monserrato, Cagliari, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Sergi
authors.affiliation: Department of Biomedical Sciences, University of Cagliari, Monserrato, Cagliari, Italy.|Department of Life and Environmental Sciences, University of Cagliari, Cagliari, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Serreli
authors.affiliation: Department of Life and Environmental Sciences, University of Cagliari, Cagliari, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Bacchetta
authors.affiliation: Institute of Environmental Geology and Geoengineering, National Research Council, Cagliari, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Milia
authors.affiliation: Institute of Environmental Geology and Geoengineering, National Research Council, Cagliari, Italy.|Department of Civil, Environmental Engineering and Architecture, University of Cagliari, Cagliari, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Cappai
authors.affiliation: Institute of Environmental Geology and Geoengineering, National Research Council, Cagliari, Italy.|Department of Civil, Environmental Engineering and Architecture, University of Cagliari, Cagliari, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Carucci
mesh_terms: D001673:Biodegradation, Environmental; D007558:Italy; D019216:Metals, Heavy / Q000032:analysis* / Q000378:metabolism*; D008906:Mining; D027927:Pistacia / Q000254:growth & development* / Q000378:metabolism / Q000382:microbiology*; D018517:Plant Roots / Q000378:metabolism* / Q000382:microbiology; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis / Q000378:metabolism
chemical_list: D019216:Metals, Heavy; D012989:Soil Pollutants
keywords: Bioaugmentation; Heavy metal; Phytoremediation; Pistacia lentiscus; Plant growth promoting bacteria