title: Biological activities of extracts from cultivated Granadilla Passiflora alata.
abstract: Research conducted in this study showed the influence of ethanol, acetone and ethyl acetate extracts of the outgrowth of cultivated Passiflora alata on microorganisms, as well as the antioxidant activity and the concentrations of total phenols, flavonoids and tannins. In vitro antimicrobial activities of extracts were studied on 27 species of microorganisms, of which 17 species of bacteria and 10 species of fungi. The strongest antimicrobial activity was detected on G+ bacteria while the activities on other species were moderate. Ethyl acetate extract showed the strongest effect. The concentrations of total phenols were examined by using Folin-Ciocalteu reagent and the obtained values ranged from 14.04 to 34.22 mg GA/g. By using aluminium chloride method, the concentrations of flavonoids were obtained and the values ranged from 33.19 to 62.30 mg RU/g. In determining the amount of tannins we used the method with buthanol-HCl reagent and the obtained value was 5.1 % of dry matter. The efficiency of antioxidation, which we identified through the reduction of DPPH, was in the range from 808.69 to 1107.79 µg/ml for a particular IC50, and AAI values were between 0.07 and 0.10. The best parameters were shown by ethanol extract. All data were statistically analyzed. Overall, extracts showed potential for further investigation and use.
authors.affiliation: Department of Biology and Ecology, Faculty of Science, University of Kragujevac, Radoja Domanovica 12, 34000 Kragujevac, Republic of Serbia.
authors.forename: Sava M
authors.initials: SM
authors.lastname: Vasic
authors.affiliation: Department of Biology and Ecology, Faculty of Science, University of Kragujevac, Radoja Domanovica 12, 34000 Kragujevac, Republic of Serbia.
authors.forename: Olgica D
authors.initials: OD
authors.lastname: Stefanovic
authors.affiliation: Department of Biomedical Sciences, State University of Novi Pazar, Vuka Karadžica bb, 36300 Novi Pazar, Republic of Serbia.
authors.forename: Braho Z
authors.initials: BZ
authors.lastname: Licina
authors.affiliation: Department of Biology and Ecology, Faculty of Science, University of Kragujevac, Radoja Domanovica 12, 34000 Kragujevac, Republic of Serbia.
authors.forename: Ivana D
authors.initials: ID
authors.lastname: Radojevic
authors.affiliation: Department of Biology and Ecology, Faculty of Science, University of Kragujevac, Radoja Domanovica 12, 34000 Kragujevac, Republic of Serbia.
authors.forename: Ljiljana R
authors.initials: LR
authors.lastname: Comic
keywords: Passiflora alata; antimicrobial capacity; antioxidant; flavonoids; phenols; tannins