title: Influence of different metals on the activation and inhibition of α-amylase from thermophilic Bacillus firmus KIBGE-IB28.
abstract: Thermophilic Bacillus firmus KIBGE-IB28 produced extracellular α-amylase at temperature 70°C. Enzyme was partially purified by ammonium sulfate precipitation with 42.80 fold purification and specific activity of 1889.6 U/mg. Effects of various metals on enzyme activity were determined and it was found that enzyme activity boosted significantly in presence of Ca(2+), K(2+), Ba(2+), Co(2+) and Ni(2+) whereas Zn(2+), Mg(2+), Na(2+) and Cu(2+) were found inhibitory at concentration 10mM.
authors.affiliation: The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi, Pakistan.
authors.forename: Raheela Rahmat
authors.initials: RR
authors.lastname: Zohra
authors.affiliation: The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi, Pakistan.
authors.forename: Shah Aliul
authors.initials: SA
authors.lastname: Qader
authors.affiliation: The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi, Pakistan.
authors.forename: Sidra
authors.initials: S
authors.lastname: Pervez
authors.affiliation: The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi, Pakistan.
authors.forename: Afsheen
authors.initials: A
authors.lastname: Aman
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D004789:Enzyme Activation / Q000187:drug effects; D008670:Metals / Q000494:pharmacology*; D000516:alpha-Amylases / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D008670:Metals; D000516:alpha-Amylases