title: Comparison between one-session root canal treatment with aPDT and two-session treatment with calcium hydroxide-based antibacterial dressing, in dog's teeth with apical periodontitis.
abstract: To evaluate one-session endodontic treatment with aPDT and two-session treatment with calcium hydroxide (CH)-based dressing in dog's teeth with apical periodontitis. After experimental induction of apical periodontitis, 48 teeth were randomly assigned to the following groups: groups OS/aPDT120d and OS/aPDT180d (one-session treatment with aPDT) and groups TS/CH120d and TS/CH180d (two-session treatment with CH-based dressing-control groups). The animals were euthanized after 120 and 180 days. After histotechnical processing, microscopic and radiographic analyses were performed. Data were analyzed by Kruskal-Wallis and Fisher's exact tests (α = 0.05). Groups TS/CHs presented repaired resorbed cemental areas, with collagen bundles and few inflammatory cells. In groups OS/aPDTs, the areas of cemental resorption were not repaired with reduced presence of cells and fibers. In the analysis of the apical closure, fluorescence microscopy and percentage of radiographic reduction of lesions, there was significant difference between groups TS/CH120d and OS/aPDT120d and between TS/CH180d and OS/aPDT180d (p < 0.05). Groups TS/CHs had weak RANKL expression and positive immunostaining for RANK and OPG. In OS/aPDT120d, there was positive immunostaining for RANKL. In OS/aPDT180d, the three osteoclastogenesis markers were expressed. The results using aPDT were worse than those obtained with two-session endodontic treatment using a CH-based dressing in teeth with apical periodontitis.
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirao Preto, SP, Brazil.
authors.forename: Lidia Regina da Costa
authors.initials: LR
authors.lastname: Hidalgo
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirao Preto, SP, Brazil.
authors.forename: Léa Assed Bezerra
authors.initials: LA
authors.lastname: da Silva
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirao Preto, SP, Brazil. nelson@forp.usp.br.|Departamento de Clínica Infantil, Faculdade de Odontologia de Ribeirão Preto, USP, Av. do Café, s/n, Monte Alegre, 14040-904, Ribeirao Preto, SP, Brasil. nelson@forp.usp.br.
authors.forename: Paulo
authors.initials: P
authors.lastname: Nelson-Filho
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirao Preto, SP, Brazil.
authors.forename: Raquel Assed Bezerra
authors.initials: RA
authors.lastname: da Silva
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirao Preto, SP, Brazil.
authors.forename: Fabrício Kitazono
authors.initials: FK
authors.lastname: de Carvalho
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirao Preto, SP, Brazil.
authors.forename: Marília Pacífico
authors.initials: MP
authors.lastname: Lucisano
authors.affiliation: Department of Bucco-Maxilo-Facial Surgery and Traumatology and Periodontology, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirao Preto, SP, Brazil.
authors.forename: Arthur Belem
authors.initials: AB
authors.lastname: Novaes
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology / Q000627:therapeutic use*; D001458:Bandages; D001862:Bone Resorption / Q000473:pathology; D002113:Calcification, Physiologic / Q000528:radiation effects; D002126:Calcium Hydroxide / Q000494:pharmacology / Q000627:therapeutic use*; D003739:Dental Cementum / Q000000981:diagnostic imaging / Q000473:pathology / Q000528:radiation effects; D004285:Dogs; D007150:Immunohistochemistry; D007249:Inflammation; D010485:Periapical Periodontitis / Q000188:drug therapy / Q000628:therapy*; D010513:Periodontal Ligament / Q000528:radiation effects; D010778:Photochemotherapy; D012390:Root Canal Therapy / Q000379:methods*; D014070:Tooth / Q000000981:diagnostic imaging / Q000187:drug effects / Q000528:radiation effects
chemical_list: D000900:Anti-Bacterial Agents; D002126:Calcium Hydroxide
keywords: Apical periodontitis; Calcium hydroxide; Photodynamic therapy; Root canal treatment