title: Recombinant chicken interferon-alpha inhibits the replication of exogenous avian leukosis virus (ALV) in DF-1 cells.
abstract: Chickeninterferon alpha (ChIFNα) belongs to type I IFNs that are important antiviral cytokines. We investigated whether ChIFNα plays a role in avian leukosis virus (ALV) infections of chickens. Firstly, we explored the immune response to ALV in vivo by measuring cytokine expression profiles in the spleens and bursas of chickens during the late stages of ALV-J infection. The results indicated that ALV-J infection could induce a mixed Th1/Th2 cytokine response by elevating levels of both interleukin-2 (IL-2) and IL-10. In contrast, tumor necrosis factor alpha (TNF-α) levels decreased in the spleen while interferon beta (IFNβ) and Toll-like receptor 7 (TLR7) expression levels in the bursa increased significantly. This indicated that ALV-J stimulates a Type I IFN response. Next, we found that different ALV subgroups or strains up-regulated chicken IFN regulatory factor 3 (ChIRF-3) promoter activity, suggesting that ALV infection could trigger Type I IFNs pathway in vitro. Accordingly, we further investigated ChIFNα antiviral effects on ALV replication in DF-1 cells by successfully expressing recombinant ChIFNα in Escherichia coli (E. coli) strain BL21. The specific activity of the purified rChIFNα protein was determined to be 4×10(7)U/mL. When added at 4000U/mL, the recombinant protein restrained ALV replication as measured by decreases in viral protein p27 levels and mRNA expression. This new reagent may be useful for prophylactic and therapeutic drug design.
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, PR China; National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, PR China; Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, PR China; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, PR China. Electronic address: daimanman1229@163.com.
authors.forename: Manman
authors.initials: M
authors.lastname: Dai
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, PR China; National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, PR China; Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, PR China; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, PR China. Electronic address: 15813365538@163.com.
authors.forename: Siyu
authors.initials: S
authors.lastname: Wu
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, PR China; National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, PR China; Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, PR China; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, PR China. Electronic address: hunanfengmin@163.com.
authors.forename: Min
authors.initials: M
authors.lastname: Feng
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, PR China; National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, PR China; Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, PR China; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, PR China. Electronic address: squarefeng@126.com.
authors.forename: Saixiang
authors.initials: S
authors.lastname: Feng
authors.affiliation: Beijing Lioson Biotechnology Ltd, Beijing, PR China. Electronic address: chaosun86@hotmail.com.
authors.forename: Chao
authors.initials: C
authors.lastname: Sun
authors.affiliation: Beijing Lioson Biotechnology Ltd, Beijing, PR China. Electronic address: bdy2006@163.com.
authors.forename: Dayong
authors.initials: D
authors.lastname: Bai
authors.affiliation: Beijing Lioson Biotechnology Ltd, Beijing, PR China. Electronic address: gumingzhu2008@163.com.
authors.forename: Mingzhu
authors.initials: M
authors.lastname: Gu
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, PR China; National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, PR China; Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, PR China; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, PR China. Electronic address: mliao@scau.edu.cn.
authors.forename: Ming
authors.initials: M
authors.lastname: Liao
authors.affiliation: College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, PR China; National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, PR China; Key Laboratory of Veterinary Vaccine Innovation of the Ministry of Agriculture, PR China; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, PR China. Electronic address: caoweish@scau.edu.cn.
authors.forename: Weisheng
authors.initials: W
authors.lastname: Cao
mesh_terms: D000818:Animals; D001353:Avian Leukosis / Q000276:immunology*; D001354:Avian Leukosis Virus / Q000276:immunology; D002645:Chickens / Q000276:immunology* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay; D016898:Interferon-alpha / Q000276:immunology* / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D011201:Poultry Diseases / Q000276:immunology / Q000821:virology; D011994:Recombinant Proteins / Q000276:immunology; D014779:Virus Replication / Q000187:drug effects / Q000502:physiology
chemical_list: D016898:Interferon-alpha; D011994:Recombinant Proteins
keywords: Chicken alpha interferon; Cytokines; Exogenous avian leukosis virus; IFN regulatory factor 3; Virus replication