title: A Long-Acting BMP-2 Release System Based on Poly(3-hydroxybutyrate) Nanoparticles Modified by Amphiphilic Phospholipid for Osteogenic Differentiation.
abstract: We explored a novel poly(3-hydroxybutyrate) (PHB) nanoparticle loaded with hydrophilic recombinant human BMP-2 with amphiphilic phospholipid (BPC-PHB NP) for a rapid-acting and long-acting delivery system of BMP-2 for osteogenic differentiation. The BPC-PHB NPs were prepared by a solvent evaporation method and showed a spherical particle with a mean particle size of 253.4 nm, mean zeta potential of -22.42 mV, and high entrapment efficiency of 77.18%, respectively. For BPC-PHB NPs, a short initial burst release of BMP-2 from NPs in 24 h was found and it has steadily risen to reach about 80% in 20 days for in vitro test. BPC-PHB NPs significantly reduced the burst release of BMP-2, as compared to that of PHB NPs loading BMP-2 without PL (B-PHB NPs). BPC-PHB NPs maintained the content of BMP-2 for a long-term osteogenic differentiation. The OCT-1 cells with BPC-PHB NPs have high ALP activity in comparison with others. The gene markers for osteogenic differentiation were significantly upregulated for sample with BPC-PHB NPs, implying that BPC-PHB NPs can be used as a rapid-acting and long-acting BMP-2 delivery system for osteogenic differentiation.
authors.affiliation: Department of Orthopaedics, The Sixth Affiliated People's Hospital, Shanghai Jiaotong University, 600 Yishan Road, Shanghai 200233, China.
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Peng
authors.affiliation: Department of Orthopaedics, The Sixth Affiliated People's Hospital, Shanghai Jiaotong University, 600 Yishan Road, Shanghai 200233, China.
authors.forename: Yunsu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Orthopaedics, The Sixth Affiliated People's Hospital, Shanghai Jiaotong University, 600 Yishan Road, Shanghai 200233, China.
authors.forename: Yamin
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Orthopaedics, The Sixth Affiliated People's Hospital, Shanghai Jiaotong University, 600 Yishan Road, Shanghai 200233, China.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Orthopaedics, The Sixth Affiliated People's Hospital, Shanghai Jiaotong University, 600 Yishan Road, Shanghai 200233, China.
authors.forename: Xianlong
authors.identifier: 0000-0001-8331-6585
authors.initials: X
authors.lastname: Zhang
mesh_terms: D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage* / Q000737:chemistry; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D016503:Drug Delivery Systems; D065546:Drug Liberation; D006801:Humans; D006885:Hydroxybutyrates / Q000008:administration & dosage / Q000737:chemistry; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry; D010006:Osteoblasts / Q000187:drug effects / Q000648:ultrastructure; D010012:Osteogenesis / Q000187:drug effects*; D010743:Phospholipids / Q000008:administration & dosage / Q000737:chemistry; D011091:Polyesters / Q000008:administration & dosage / Q000737:chemistry; D000091402:Prohibitins; D013501:Surface-Active Agents / Q000008:administration & dosage / Q000737:chemistry; D000094122:Polyhydroxybutyrates
chemical_list: D055396:Bone Morphogenetic Protein 2; D006885:Hydroxybutyrates; D010743:Phospholipids; D011091:Polyesters; D000091402:Prohibitins; D013501:Surface-Active Agents; C000717328:PHB protein, human; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates