title: Effect of chemical stress on germination of cv Dalia bean (Phaseolus vularis L.) as an alternative to increase antioxidant and nutraceutical compounds in sprouts.
abstract: The aim of this study was to determine the effect of chitosan (CH), salicylic acid (SA) and hydrogen peroxide (H2O2) at different concentrations on the antinutritional and nutraceutical content, as well as the antioxidant capacity of bean sprouts (cv Dalia). All elicitors at medium and high concentrations reduced the antinutritional content of lectins (48%), trypsin inhibitor (57%), amylase inhibitor (49%) and phytic acid (56%). Sprouts treated with CH, SA and H2O2 (7μM; 1 and 2mM, and 30mM respectively) increased the content of phenolic compounds (1.8-fold), total flavonoids (3-fold), saponins (1.8-fold) and antioxidant capacity (37%). Furthermore, the UPLC-ESI-MS/MS analysis showed an increase of several nutraceutical compounds in bean sprouts treated with SA such as coumaric (8.5-fold), salicylic (115-fold), gallic (25-fold) and caffeic (1.7-fold) acids, as well as epigallocatechin (63-fold), rutin (41-fold) and quercetin (16.6-fold) flavonoids. The application of elicitors in bean seed during sprouting enhances their nutraceutical properties.
authors.affiliation: Research and Graduate Studies in Food Science, Faculty of Chemistry, Autonomous University of Queretaro, Queretaro 76010, Mexico.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Mendoza-Sánchez
authors.affiliation: Biosystems Engineering Group, Faculty of Engineering, Autonomous University of Queretaro, Queretaro 76010, Mexico.
authors.forename: Ramón G
authors.initials: RG
authors.lastname: Guevara-González
authors.affiliation: Research and Graduate Studies in Food Science, Faculty of Chemistry, Autonomous University of Queretaro, Queretaro 76010, Mexico.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Castaño-Tostado
authors.affiliation: Research and Graduate Studies in Food Science, Faculty of Chemistry, Autonomous University of Queretaro, Queretaro 76010, Mexico.
authors.forename: Edmundo M
authors.initials: EM
authors.lastname: Mercado-Silva
authors.affiliation: Campo Experiemental Bajío (CEBAJ-INIFAP), Km 6. Carretera San Miguel de Allende, 38010 Celaya, Mexico.
authors.forename: Jorge A
authors.initials: JA
authors.lastname: Acosta-Gallegos
authors.affiliation: Instituto Tecnológico de Durango, Departamento de ingeniería Química y bioquímica, Felipe Pescador 1830 Ote., Col. Nueva Vizcaya, Durango, Dgo. C.P. 34080, Mexico.
authors.forename: Nuria E
authors.initials: NE
authors.lastname: Rocha-Guzmán
authors.affiliation: Research and Graduate Studies in Food Science, Faculty of Chemistry, Autonomous University of Queretaro, Queretaro 76010, Mexico. Electronic address: rrcamachomx@yahoo.com.mx.
authors.forename: Rosalía
authors.initials: R
authors.lastname: Reynoso-Camacho
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D002392:Catechin / Q000031:analogs & derivatives / Q000032:analysis; D048271:Chitosan / Q000494:pharmacology*; D019587:Dietary Supplements / Q000032:analysis*; D005419:Flavonoids / Q000032:analysis; D018525:Germination / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D027805:Phaseolus / Q000737:chemistry / Q000187:drug effects*; D010636:Phenols / Q000032:analysis; D010833:Phytic Acid / Q000032:analysis; D011794:Quercetin / Q000032:analysis; D020156:Salicylic Acid / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry / Q000187:drug effects; D053719:Tandem Mass Spectrometry
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D010833:Phytic Acid; D002392:Catechin; D048271:Chitosan; D011794:Quercetin; D006861:Hydrogen Peroxide; C057580:gallocatechol; D020156:Salicylic Acid
keywords: Antioxidant activity; Bean sprouts; Chemical stress; Chitosan (PubChem CID: 21896651); Elicitors; Hydrogen peroxide (PubChem CID: 784); Polyphenolic compounds; Salicylic acid (PubChem CID: 338)