title: Effect of supplementation of yeast with bacteriocin andLactobacillusculture on growth performance, cecal fermentation, microbiota composition, and blood characteristics in broiler chickens.
abstract: OBJECTIVE
  The aim of the present study was to investigate the effect of yeast with bacteriocin andLactobacilluscultures (mixture ofLactobacillus agilisBCRC 10436 andLactobacillus reuteriBCRC 17476) supplements, alone or in combination, on broiler chicken performance.
  
  
  METHODS
  A total of 300, 1-d-old healthy broiler chickens were randomly divided into five treatment groups: i) basal diet (control), ii) basal diet+0.25% yeast (Saccharomyces cerevisiae) (YC), iii) basal diet+0.25% yeast with bacteriocin (BA), iv) basal diet+Lactobacilluscultures (LAB), and v) basal diet +0.25% yeast with bacteriocin+Lactobacilluscultures (BA+LAB). Growth performance, cecal microbiota, cecal fermentation products, and blood biochemistry parameters were determined when chickens were 21 and 35 d old.
  
  
  RESULTS
  The supplementation of YC, BA, and BA+LAB resulted in a significantly better feed conversion rate (FCR) than that of the control group during 1 to 21 d (p<0.05). The LAB supplementation had a significant effect on the presence ofLactobacillusin the ceca at 35 d. None of the supplements had an effect on relative numbers ofL. agilisandL. reuterat 21 d, but the BA supplementation resulted in the decrease of bothLactobacillusstrains at 35 d. The BA+LAB supplementation resulted in higher short chain fatty acid (SCFA) in the ceca, but LAB supplementation significantly decreased the SCFA at 35 d (p<0.05). All treatments tended to decrease ammonia concentration in the ceca at 21 d, especially in the LAB treatment group. The BA supplementation alone decreased the triacylglycerol (TG) concentration significantly at 21 d (p<0.05), but the synergistic effect of BA and LAB supplementation was required to reduce the TG concentration at 35 d. The YC supplementation tended to increase the plasma cholesterol at 21 d and 35 d. However, the BA supplementation significantly decreased the cholesterol and low density lipoprotein cholesterol level at 35 d. In conclusion, the BA+LAB supplementation was beneficial to body weight gain and FCR of broiler chickens.
  
  
  CONCLUSIONS
  The effect of BA and LAB supplementation may be a result of the growth of lactic acid bacteria enhancement and physiological characterization of bacteriocin, and it suggests that the BA and LAB supplementation level orLactobacillusstrain selection should be integrated in future supplementation designs.
authors.affiliation: Department of Animal Science and Technology, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: C Y
authors.initials: CY
authors.lastname: Chen
authors.affiliation: Department of Animal Science and Technology, National Taiwan University, Taipei 10617, Taiwan.
authors.forename: S W
authors.initials: SW
authors.lastname: Chen
authors.affiliation: Department of Animal Science, Chinese Culture University, Taipei 11114, Taiwan.
authors.forename: H T
authors.initials: HT
authors.lastname: Wang
keywords: Bacteriocin; Broiler; Cecal Fermentation; Growth Performance; Lactic Acid Bacteria