title: Application of flat panel OLED display technology for the point-of-care detection of circulating cancer biomarkers.
abstract: Point-of-care molecular diagnostics can provide efficient and cost-effective medical care, and they have the potential to fundamentally change our approach to global health. However, most existing approaches are not scalable to include multiple biomarkers. As a solution, we have combined commercial flat panel OLED display technology with protein microarray technology to enable high-density fluorescent, programmable, multiplexed biorecognition in a compact and disposable configuration with clinical-level sensitivity. Our approach leverages advances in commercial display technology to reduce pre-functionalized biosensor substrate costs to pennies per cm(2). Here, we demonstrate quantitative detection of IgG antibodies to multiple viral antigens in patient serum samples with detection limits for human IgG in the 10 pg/mL range. We also demonstrate multiplexed detection of antibodies to the HPV16 proteins E2, E6, and E7, which are circulating biomarkers for cervical as well as head and neck cancers.
authors.affiliation: Center for Personalized Diagnostics, Biodesign Institute at Arizona State University, Tempe, AZ 85281 USA.
authors.forename: Benjamin A
authors.initials: BA
authors.lastname: Katchman
authors.affiliation: Flexible Electronics and Display Center at Arizona State University, Tempe, AZ 85284 USA.
authors.forename: Joseph T
authors.initials: JT
authors.lastname: Smith
authors.affiliation: School of Electrical Engineering at Arizona State University, Tempe, AZ 85281 USA.
authors.forename: Uwadiae
authors.initials: U
authors.lastname: Obahiagbon
authors.affiliation: Center for Personalized Diagnostics, Biodesign Institute at Arizona State University, Tempe, AZ 85281 USA.
authors.forename: Sailaja
authors.initials: S
authors.lastname: Kesiraju
authors.affiliation: Flexible Electronics and Display Center at Arizona State University, Tempe, AZ 85284 USA.
authors.forename: Yong-Kyun
authors.initials: YK
authors.lastname: Lee
authors.affiliation: Flexible Electronics and Display Center at Arizona State University, Tempe, AZ 85284 USA.
authors.forename: Barry
authors.initials: B
authors.lastname: O'Brien
authors.affiliation: Flexible Electronics and Display Center at Arizona State University, Tempe, AZ 85284 USA.
authors.forename: Korhan
authors.initials: K
authors.lastname: Kaftanoglu
authors.affiliation: School of Electrical Engineering at Arizona State University, Tempe, AZ 85281 USA.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Blain Christen
authors.affiliation: Center for Personalized Diagnostics, Biodesign Institute at Arizona State University, Tempe, AZ 85281 USA.
authors.forename: Karen S
authors.initials: KS
authors.lastname: Anderson
mesh_terms: D000906:Antibodies / Q000097:blood; D014408:Biomarkers, Tumor / Q000097:blood*; D015374:Biosensing Techniques / Q000379:methods*; D004268:DNA-Binding Proteins / Q000097:blood; D005260:Female; D052162:Human papillomavirus 16 / Q000302:isolation & purification / Q000472:pathogenicity; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D057230:Limit of Detection; D009856:Oncogene Proteins, Viral / Q000097:blood; D050725:Papillomavirus E7 Proteins / Q000097:blood; D030361:Papillomavirus Infections / Q000097:blood* / Q000473:pathology; D057089:Pathology, Molecular; D019095:Point-of-Care Systems; D040081:Protein Array Analysis; D012097:Repressor Proteins / Q000097:blood; D002583:Uterine Cervical Neoplasms / Q000097:blood* / Q000473:pathology
chemical_list: D000906:Antibodies; D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; C054855:E2 protein, Human papillomavirus type 16; C068651:E6 protein, Human papillomavirus type 16; D007074:Immunoglobulin G; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012097:Repressor Proteins; C059731:oncogene protein E7, Human papillomavirus type 16