title: Protective effects of Urtica dioica L. on experimental testicular ischaemia reperfusion injury in rats.
abstract: In this study, it was aimed to examine the effects of Urtica dioica L. (UD) that has antioxidant feature in the experimental testicular I/R model in rats in terms of anti-apoptotic and antioxidative effects. In our study, 24 male rats were divided into three groups: control group, I/R group and I/R + UD (2 mg kg-1) group. Seminiferous tubule calibre measurement, Johnson score, haematoxylin-eosin staining, proliferative cell nucleus antigen (PCNA) immunohistochemical staining and TUNEL as histopathological have been conducted. The structural deterioration in the testicular on I/R group has reduced after the treatment of UD. Our data indicate a significant reduction in the activity of in situ identification of apoptosis using terminal dUTP nick end labelling (TUNEL), and there was a rise in the expression of proliferating cell nuclear antigen (PCNA) in testis tissues of UD-treated rats in the I/R group. The I/R + UD group showed a decrease in malondialdehyde levels and an increase in the activities of superoxide dismutase, catalase and glutathione peroxidase in comparison with the I/R group. It could be concluded that protective effects of UD on the I/R testicles are via reduction of histological damage, apoptosis, oxidative stress and lipid peroxidation.
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, University of Namik Kemal, Tekirdag, Turkey.
authors.forename: C
authors.initials: C
authors.lastname: Aktas
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, University of Namik Kemal, Tekirdag, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Erboga
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, University of Namik Kemal, Tekirdag, Turkey.
authors.forename: Z
authors.initials: Z
authors.lastname: Fidanol Erboga
authors.affiliation: Technology Research and Development Application and Research Center, University of Trakya, Edirne, Turkey.
authors.forename: Y
authors.initials: Y
authors.lastname: Bozdemir Donmez
authors.affiliation: Department of Biostatistics, Faculty of Medicine, University of Namik Kemal, Tekirdag, Turkey.
authors.forename: B
authors.initials: B
authors.lastname: Topcu
authors.affiliation: Department of Biochemistry, Faculty of Medicine, University of Namik Kemal, Tekirdag, Turkey.
authors.forename: A
authors.initials: A
authors.lastname: Gurel
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects*; D002374:Catalase / Q000032:analysis; D049109:Cell Proliferation; D004195:Disease Models, Animal; D005979:Glutathione Peroxidase / Q000032:analysis; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000627:therapeutic use*; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D051381:Rats; D015427:Reperfusion Injury / Q000188:drug therapy*; D012639:Seeds; D012671:Seminiferous Tubules / Q000033:anatomy & histology; D013482:Superoxide Dismutase / Q000032:analysis; D013737:Testis / Q000033:anatomy & histology / Q000187:drug effects* / Q000378:metabolism; D026982:Urtica dioica / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D018809:Proliferating Cell Nuclear Antigen; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: Urtica dioica L.; ischaemia and reperfusion; testis-apoptosis-oxidative stress