title: Lactoferrin Adsorbed onto Biomimetic Hydroxyapatite Nanocrystals Controlling - In Vivo - the Helicobacter pylori Infection.
abstract: BACKGROUND
  The resistance of Helicobacter pylori to the antibiotic therapy poses the problem to discover new therapeutic approaches. Recently it has been stated that antibacterial, immunomodulatory, and antioxidant properties of lactoferrin are increased when this protein is surface-linked to biomimetic hydroxyapatite nanocrystals.
  
  
  OBJECTIVE
  Based on these knowledge, the aim of the study was to investigate the efficacy of lactoferrin delivered by biomimetic hydroxyapatite nanoparticles with cell free supernatant from probiotic Lactobacillus paracasei as an alternative therapy against Helicobacter pylori infection.
  
  
  METHODS
  Antibacterial and antinflammatory properties, humoral antibody induction, histopathological analysis and absence of side effects were evaluated in both in vitro and in vivo studies.
  
  
  RESULTS
  The tests carried out have been demonstrated better performance of lactoferrin delivered by biomimetic hydroxyapatite nanoparticles combined with cell free supernatant from probiotic Lactobacillus paracasei compared to both lactoferrin and probiotic alone or pooled.
  
  
  CONCLUSIONS
  These findings indicate the effectiveness and safety of our proposed therapy as alternative treatment for Helicobacter pylori infection.
authors.affiliation: Department of Agriculture, University of Naples "Federico II", Portici (Naples), Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Fulgione
authors.affiliation: Department of Agriculture, University of Naples "Federico II", Portici (Naples), Italy.
authors.forename: Nunzia
authors.initials: N
authors.lastname: Nocerino
authors.affiliation: Department of Agriculture, University of Naples "Federico II", Portici (Naples), Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Iannaccone
authors.affiliation: Department of Veterinary Medicine and Animal Productions, Division of Infectious Diseases, University of Naples "Federico II", Naples, Italy.
authors.forename: Sante
authors.initials: S
authors.lastname: Roperto
authors.affiliation: Department of Food Inspection, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici (Naples), Italy.
authors.forename: Federico
authors.initials: F
authors.lastname: Capuano
authors.affiliation: Department of Chemistry "G. Ciamician", Alma Mater Studiorum, University of Bologna, LEBSC, Bologna, Italy.
authors.forename: Norberto
authors.initials: N
authors.lastname: Roveri
authors.affiliation: Department of Chemistry "G. Ciamician", Alma Mater Studiorum, University of Bologna, LEBSC, Bologna, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Lelli
authors.affiliation: Department of Agriculture, University of Naples "Federico II", Portici (Naples), Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Crasto
authors.affiliation: Advanced Biomedical Science Department, University of Naples "Federico II", Naples, Italy.
authors.forename: Armando
authors.initials: A
authors.lastname: Calogero
authors.affiliation: Department of Experimental Medicine, Division of Clinical Bacteriology, Second University of Naples, Naples, Italy.
authors.forename: Argenia Paola
authors.initials: AP
authors.lastname: Pilloni
authors.affiliation: Department of Agriculture, University of Naples "Federico II", Portici (Naples), Italy.
authors.forename: Rosanna
authors.initials: R
authors.lastname: Capparelli
mesh_terms: D000327:Adsorption; D000818:Animals; D000890:Anti-Infective Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D040761:Biomimetic Materials / Q000737:chemistry; D017077:Culture Media, Conditioned / Q000494:pharmacology; D016207:Cytokines / Q000378:metabolism; D016503:Drug Delivery Systems / Q000379:methods; D017886:Durapatite / Q000737:chemistry*; D005753:Gastric Mucosa / Q000378:metabolism; D016481:Helicobacter Infections / Q000382:microbiology* / Q000517:prevention & control; D016480:Helicobacter pylori / Q000187:drug effects / Q000502:physiology*; D054884:Host-Pathogen Interactions / Q000187:drug effects; D000069980:Lacticaseibacillus paracasei / Q000502:physiology; D007781:Lactoferrin / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology; D008807:Mice, Inbred BALB C; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D019936:Probiotics / Q000008:administration & dosage / Q000494:pharmacology; D013270:Stomach / Q000187:drug effects / Q000382:microbiology; D016896:Treatment Outcome; D014961:X-Ray Diffraction
chemical_list: D000890:Anti-Infective Agents; D017077:Culture Media, Conditioned; D016207:Cytokines; D017886:Durapatite; D007781:Lactoferrin