title: Gene Mapping of a Mutant Mungbean (Vigna radiata L.) Using New Molecular Markers Suggests a Gene Encoding a YUC4-like Protein Regulates the Chasmogamous Flower Trait.
abstract: Mungbean (Vigna radiata L.) is a cleistogamous plant in which flowers are pollinated before they open, which prevents yield improvements through heterosis. We previously generated a chasmogamous mutant (CM) mungbean in which open flowers are pollinated. In this study, we developed insertion/deletion (indel) markers based on the transcriptome differences between CM and Sulu-1 (i.e., normal flowering) plants. An F2 population derived from a cross between CM and Sulu-1 was used for gene mapping. Segregation analyses revealed that a single recessive gene regulates the production of chasmogamous flowers. Using newly developed indel and simple sequence repeat markers, the cha gene responsible for the chasmogamous flower trait was mapped to a 277.1-kb segment on chromosome 6. Twelve candidate genes were detected in this segment, including Vradi06g12650, which encodes a YUCCA family protein associated with floral development. A single base pair deletion producing a frame-shift mutation and a premature stop codon in Vradi06g12650 was detected only in CM plants. This suggested that Vradi06g12650 is a cha candidate gene. Our results provide important information for the molecular breeding of chasmogamous mungbean lines, which may serve as new genetic resources for hybrid cultivar development.
authors.affiliation: Institute of Vegetable Crops, Jiangsu Academy of Agricultural SciencesNanjing, China; Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart UniversityNakhon Pathom, Thailand.
authors.forename: Jingbin
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University Nakhon Pathom, Thailand.
authors.forename: Prakit
authors.initials: P
authors.lastname: Somta
authors.affiliation: Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences Nanjing, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.affiliation: Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences Nanjing, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Cui
authors.affiliation: Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences Nanjing, China.
authors.forename: Xingxing
authors.initials: X
authors.lastname: Yuan
authors.affiliation: Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University Nakhon Pathom, Thailand.
authors.forename: Peerasak
authors.initials: P
authors.lastname: Srinives
keywords: Vigna radiata; chasmogamy; gene mapping; hybrid development; mungbean; outcrossing