title: [Expression of p53, p21, PCNA and COX-2 and its relationship with recurrence in the early-stage laryngeal cancer with negative surgical margin].
abstract: OBJECTIVE
  To explore the relation between the expression of p53, p21, PCNA and COX-2 and local recurrence in resection margins of laryngeal carcinoma operation.
  
  
  METHODS
  SElect 98 patients with laryngeal carcinoma, who came to our hospital from Nov, 2005 to Dec, 2010. Diagnosed with early laryngeal squamous cell carcinoma by a pathological examination, all these patients received CO2 laser surgery to cut the entire tumors. Keep the primary lesion and resection margin tissues after that and take 5 mm peritumoral tissue as a sample of operation resection margin. With the chemical method of SP, record in detail the local recurrence condition by clinical diagnosis every 3-6 months or telephone follow-up for 2 years.
  
  
  RESULTS
  (1)The positive rate of p53, p21, PCNA and COX-2 in the tumor tissues of the 98 patients with laryngeal carcinoma are 65. 3%, 52. 0%, 70. 4% and 69. 4%. The positive rate of p53, p21, PCNA and COX-2 in their resection margin tissues are 23. 5%, 39. 8%, 32. 7% and 30. 6%. So there is no relation between the expression of p53, p21, PCNA and COX-2 in tumor tissue and tumorous grading and TNM staging. (2)There appeared 17 cases of local recurrence in two years, with a recurrence rate of 17. 3%. The recurrence rate of patients with a positive expression of p53, p21, PCNA and COX-2 in resection margin tissue is higher than those with a negative expression(P<0. 05). Among positive cases, the recurrence rate of patients with double positive results is obviously higher than those with single positive results(P< 0. 05). (3)The expression of p21 in p53 positive resection margin tissue is greatly higher than that in negative tissue. The expression of PCNA in p53 positive resection margin tissue is greatly higher than that in negative tissue (P<0. 01); however, the PCNA positive expression in p53 positive cut edge organization rather than in the expression of negative cut edge groups(P>0. 05).
  
  
  CONCLUSIONS
  Through the discussion on the effect of p53, p21, PC- NA and COX-2 in the process of laryngeal carcinoma cell proliferation and local recurrence, the study proposes that biochemical metabolism and molecular structure level abnormal expression occur before the change of cell morphology apparent, and suggests that positive index should be examined regularly and effective foreseeability intervention can be applied to patients with positive expression.
authors.forename: Ronghua
authors.initials: R
authors.lastname: Zeng
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000378:metabolism; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism*; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D006258:Head and Neck Neoplasms / Q000378:metabolism; D006801:Humans; D007822:Laryngeal Neoplasms / Q000378:metabolism*; D009364:Neoplasm Recurrence, Local; D009367:Neoplasm Staging; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D000077195:Squamous Cell Carcinoma of Head and Neck; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D018809:Proliferating Cell Nuclear Antigen; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human