title: Pterostilbene-induced changes in gut microbiota composition in relation to obesity.
abstract: Nutritional interventions based on the use of natural bioactive compounds might offer new possibilities for reshaping obesity-associated bacterial dysregulation or dysbiosis and improving health. We evaluated whether pterostilbene supplementation could induce changes in gut microbiota composition and whether these modifications were associated with improvements in metabolic variables.
  
  
  
  Zucker (fa/fa) rats were given a standard diet supplemented (n = 10) or not (n = 9) with pterostilbene (15 mg/kg body weight/day) by oral gavage for 6 weeks. Faucal samples at the beginning and at the end of the intervention period were analyzed by Illumina Mi-Seq sequencing approach. Pterostilbene exerted protective antiobesity effects, improved metabolic function (insulin sensitivity), and induced structural changes in gut microbiota composition. A decrease in the levels of Firmicutes and an increase in Verrucomicrobia phyla were detected in the pterostilbene-treated group. Bacterial species belonging to genera Akkermansia and Odoribacter were also increased. A strong inverse correlation between Akkermansia muciniphila and body weight was evidenced. Odoribacter splanchnicus showed a negative correlation with adiposity.
  
  
  
  Pterostilbene modifies intestinal bacteria composition toward a healthier microbial profile and suggests that the antiobesity effects induced in Zucker rats could be associated with an enrichment of the mucin-degrading bacterial members, namely Akkermansia and Odoribacter genus.
authors.affiliation: Department of Nutrition, Food Science and Physiology, and Centre for Nutrition Research University of Navarra, Pamplona, Spain.
authors.forename: Usune
authors.initials: U
authors.lastname: Etxeberria
authors.affiliation: Department of Gastroenterology, Donostia Hospital and Biodonostia Institute, University of the Basque Country (UPV/EHU), San Sebastián, Spain.|CIBER Hepatic and Digestive Pathologies (CIBERehd), Institute of Health Carlos III, Madrid, Spain.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Hijona
authors.affiliation: CIBER Obesity and Physiopathology of Nutrition (CIBERobn), Institute of Health Carlos III, Madrid, Spain.|Nutrition and Obesity Group, Department of Nutrition and Food Sciences, Faculty of Pharmacy, University of the Basque Country (UPV/EHU) and Lucio Lascaray Research Centre, Vitoria, Spain.
authors.forename: Leixuri
authors.initials: L
authors.lastname: Aguirre
authors.affiliation: Department of Nutrition, Food Science and Physiology, and Centre for Nutrition Research University of Navarra, Pamplona, Spain.|CIBER Obesity and Physiopathology of Nutrition (CIBERobn), Institute of Health Carlos III, Madrid, Spain.
authors.forename: Fermin I
authors.initials: FI
authors.lastname: Milagro
authors.affiliation: Department of Gastroenterology, Donostia Hospital and Biodonostia Institute, University of the Basque Country (UPV/EHU), San Sebastián, Spain.|CIBER Hepatic and Digestive Pathologies (CIBERehd), Institute of Health Carlos III, Madrid, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Bujanda
authors.affiliation: United States Department of Agriculture, Agricultural Research Service Natural Products Utilization Research Unit, University, MS, USA.
authors.forename: Agnes M
authors.initials: AM
authors.lastname: Rimando
authors.affiliation: Department of Nutrition, Food Science and Physiology, and Centre for Nutrition Research University of Navarra, Pamplona, Spain.|CIBER Obesity and Physiopathology of Nutrition (CIBERobn), Institute of Health Carlos III, Madrid, Spain.
authors.forename: José Alfredo
authors.initials: JA
authors.lastname: Martínez
authors.affiliation: CIBER Obesity and Physiopathology of Nutrition (CIBERobn), Institute of Health Carlos III, Madrid, Spain.|Nutrition and Obesity Group, Department of Nutrition and Food Sciences, Faculty of Pharmacy, University of the Basque Country (UPV/EHU) and Lucio Lascaray Research Centre, Vitoria, Spain.
authors.forename: María P
authors.initials: MP
authors.lastname: Portillo
mesh_terms: D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D019587:Dietary Supplements; D064806:Dysbiosis / Q000178:diet therapy; D000069196:Gastrointestinal Microbiome / Q000187:drug effects*; D009765:Obesity / Q000178:diet therapy* / Q000378:metabolism*; D011924:Rats, Zucker; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D019440:Anti-Obesity Agents; D013267:Stilbenes; C107773:pterostilbene
keywords: Akkermansia; Insulin sensitivity; Odoribacter; Polyphenols; Verrucomicrobia