title: Genome-wide microRNA Expression Profiling in Primary Tumors and Matched Liver Metastasis of Patients with Colorectal Cancer.
abstract: BACKGROUND
  Primary tumor spread to the liver is the major cause of disease progression and death in patients with colorectal cancer (CRC). MicroRNAs (miRNAs) are small non-coding RNAs that are involved in cancer development and progression, but their role in metastasis has not been extensively investigated.
  
  
  METHODS
  Firstly, expression profiling of 752 miRNAs in 20 primary tumors and their corresponding liver metastases was performed. Secondly, validation of the results was carried out on an independent cohort of 66 patients with metastatic CRC using reverse transcription-quantitative polymerase chain (RT-qPCR) reaction.
  
  
  RESULTS
  In total, 33 miRNAs were found to be significantly deregulated in liver metastases compared to their primary tumors. Fifteen miRNAs were chosen for subsequent validation, which confirmed significantly reduced expression of miR-143, miR 10b, and miR-28-5p, and increased expression of miR 122, miR-122*, and miR 885-5p in the tissue of liver metastases.
  
  
  CONCLUSIONS
  These results indicate that miRNAs could serve as new therapeutic targets in patients with metastatic CRC.
authors.affiliation: Central European Institute of Technology, Masaryk University, Brno, Czech Republic Department of Comprehensive Cancer Care, Masaryk Memorial Cancer Institute, Brno, Czech Republic.
authors.forename: Petra
authors.initials: P
authors.lastname: Vychytilova-Faltejskova
authors.affiliation: Department of Biology, Faculty of Medicine in Pilsen, Charles University in Prague, Prague, Czech Republic the Biomedical Center, Faculty of Medicine in Pilsen, Charles University in Prague, Prague, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Pesta
authors.affiliation: Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Radova
authors.affiliation: the Biomedical Center, Faculty of Medicine in Pilsen, Charles University in Prague, Prague, Czech Republic Department of Surgery, Faculty of Medicine in Pilsen, Charles University in Prague, Prague, Czech Republic.
authors.forename: Vaclav
authors.initials: V
authors.lastname: Liska
authors.affiliation: Department of Pathology, Faculty of Medicine in Pilsen, Charles University in Prague, Prague, Czech Republic.
authors.forename: Ondrej
authors.initials: O
authors.lastname: Daum
authors.affiliation: Department of Surgery, University Hospital Brno, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Kala
authors.affiliation: Central European Institute of Technology, Masaryk University, Brno, Czech Republic Department of Comprehensive Cancer Care, Masaryk Memorial Cancer Institute, Brno, Czech Republic.
authors.forename: Marek
authors.initials: M
authors.lastname: Svoboda
authors.affiliation: Department of Comprehensive Cancer Care, Masaryk Memorial Cancer Institute, Brno, Czech Republic on.slaby@gmail.com kiss@mou.cz.
authors.forename: Igor
authors.initials: I
authors.lastname: Kiss
authors.affiliation: Central European Institute of Technology, Masaryk University, Brno, Czech Republic Department of Comprehensive Cancer Care, Masaryk Memorial Cancer Institute, Brno, Czech Republic on.slaby@gmail.com kiss@mou.cz.
authors.forename: Ondrej
authors.initials: O
authors.lastname: Slaby
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016000:Cluster Analysis; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D055106:Genome-Wide Association Study; D006801:Humans; D008113:Liver Neoplasms / Q000556:secondary*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D015203:Reproducibility of Results; D059467:Transcriptome
chemical_list: D035683:MicroRNAs
keywords: Colorectal cancer; liver metastasis; microRNA