title: Prevalence and behavior of multidrug-resistant shiga toxin-producing Escherichia coli, enteropathogenic E. coli and enterotoxigenic E. coli on coriander.
abstract: The prevalence and behavior of multidrug-resistant diarrheagenic Escherichia coli pathotypes on coriander was determined. One hundred coriander samples were collected from markets. Generic E. coli were determined using the most probable number procedure. Diarrheagenic E. coli pathotypes (DEPs) were identified using two multiplex polymerase chain reaction procedures. Susceptibility to sixteen antibiotics was tested for the isolated DEPs strains by standard test. The behavior of multidrug-resistant DEPs isolated from coriander was determined on coriander leaves and chopped coriander at 25°± 2 °C and 3°± 2 °C. Generic E. coli and DEPs were identified, respectively, in 43 and 7% of samples. Nine DEPs strains were isolated from positive coriander samples. The identified DEPs included Shiga toxin-producing E. coli (STEC, 4%) enterotoxigenic E. coli (ETEC, 2%) and enteropathogenic E. coli (EPEC, 1%). All isolated DEPs strains exhibited multi-resistance to antibiotics. On inoculated coriander leaves stored at 25°± 2 °C or 3°± 2 °C, no growth was observed for multidrug-resistant DEPs strains. However, multidrug-resistant DEPs strains grew in chopped coriander: after 24 h at 25° ± 2 °C, DEPs strains had grown to approximately 3 log CFU/g. However, at 3°± 2 °C the bacterial growth was inhibited. To the best of our knowledge, this is the first report of the presence and behavior of multidrug-resistant STEC, ETEC and EPEC on coriander and chopped coriander.
authors.affiliation: Centro de Investigaciones Químicas, Instituto de Ciencias Básicas e Ingeniería, Universidad Autónoma del Estado de Hidalgo, Ciudad del Conocimiento, Carretera Pachuca-Tulancingo Km. 4.5. 42184, Mineral de la Reforma, Hidalgo, Mexico.
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Gómez-Aldapa
authors.affiliation: Centro de Investigaciones Químicas, Instituto de Ciencias Básicas e Ingeniería, Universidad Autónoma del Estado de Hidalgo, Ciudad del Conocimiento, Carretera Pachuca-Tulancingo Km. 4.5. 42184, Mineral de la Reforma, Hidalgo, Mexico.
authors.forename: Jesús A
authors.initials: JA
authors.lastname: Segovia-Cruz
authors.affiliation: Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas-IPN, Prolongación Carpio y Plan de Ayala s/n, Col. Casco de Santo Tomas, Del. Miguel Hidalgo, 11340, México, D.F., Mexico.
authors.forename: Jorge F
authors.initials: JF
authors.lastname: Cerna-Cortes
authors.affiliation: Centro de Investigaciones Químicas, Instituto de Ciencias Básicas e Ingeniería, Universidad Autónoma del Estado de Hidalgo, Ciudad del Conocimiento, Carretera Pachuca-Tulancingo Km. 4.5. 42184, Mineral de la Reforma, Hidalgo, Mexico.
authors.forename: Esmeralda
authors.initials: E
authors.lastname: Rangel-Vargas
authors.affiliation: Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas-IPN, Prolongación Carpio y Plan de Ayala s/n, Col. Casco de Santo Tomas, Del. Miguel Hidalgo, 11340, México, D.F., Mexico.
authors.forename: Laura P
authors.initials: LP
authors.lastname: Salas-Rangel
authors.affiliation: Centro de Investigaciones Químicas, Instituto de Ciencias Básicas e Ingeniería, Universidad Autónoma del Estado de Hidalgo, Ciudad del Conocimiento, Carretera Pachuca-Tulancingo Km. 4.5. 42184, Mineral de la Reforma, Hidalgo, Mexico.
authors.forename: Eduardo J
authors.initials: EJ
authors.lastname: Gutiérrez-Alcántara
authors.affiliation: Centro de Investigaciones Químicas, Instituto de Ciencias Básicas e Ingeniería, Universidad Autónoma del Estado de Hidalgo, Ciudad del Conocimiento, Carretera Pachuca-Tulancingo Km. 4.5. 42184, Mineral de la Reforma, Hidalgo, Mexico. Electronic address: jcastro@uaeh.edu.mx.
authors.forename: Javier
authors.initials: J
authors.lastname: Castro-Rosas
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D058491:Bacterial Load; D028526:Coriandrum / Q000382:microbiology*; D024901:Drug Resistance, Multiple, Bacterial; D054308:Enteropathogenic Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D054307:Enterotoxigenic Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D008800:Mexico; D016133:Polymerase Chain Reaction; D054323:Shiga-Toxigenic Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D013696:Temperature
chemical_list: D000900:Anti-Bacterial Agents
keywords: Antibiotic; Coriander; EPEC; ETEC; Multidrug-resistant; STEC