title: [Determination of ginkgolic acids in the ginkgo biloba extract and its preparations by ultra high performance liquid chromatography-triple quadrupole mass spectrometry].
abstract: An analytical method was developed for the determination of ginkgolic acids in ginkgo biloba extract and its preparations by ultra high performance liquid chromatography-triple quadrupole mass spectrometry. A chromatographic column, Agilent Poroshell 120 EC-C18 (50 mm x 3. 0 mm, 2.7 µm), was used with methanol-l% acetic acid (90 :10, v/v) as the mobile phase. The ginkgo acids were detected by electrospray ionization mass spectrometry in negative mode with multiple reaction monitoring (MRM) mode. Ginkgo acids C13:0, C15:1 and C17:1 possessed good linear correlations in the mass concentration range from 2 to 200 µg/L, with the correlation coefficients more than 0. 999. The mean recoveries at the spiked levels of 5, 20 and 100 µg/L were in the range of 86. 3%-114. 3%, and the RSDs were 0. 5%-13. 6%. The limits of detection and quantification were 0. 003-0. 08 µg/g and 0. 01-0. 19 µg/g, respectively. The method was successfully applied to the analysis of actual samples.
authors.forename: Jian
authors.initials: J
authors.lastname: Sun
authors.forename: Limin
authors.initials: L
authors.lastname: Li
authors.forename: Qing
authors.initials: Q
authors.lastname: Hu
authors.forename: Xiuhong
authors.initials: X
authors.lastname: Mao
authors.forename: Shen
authors.initials: S
authors.lastname: Ji
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D020441:Ginkgo biloba / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis*; D012459:Salicylates / Q000032:analysis*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D012459:Salicylates; C112485:ginkgolic acid