title: Self-paired monoclonal antibody lateral flow immunoassay strip for rapid detection of Acidovorax avenae subsp. citrulli.
abstract: We screened a highly specific monoclonal antibody (McAb), named 6D, against Acidovorax avenae subsp. citrulli (Aac). Single McAb 6D was used as both nanogold-labeled antibody and test antibody to develop a single self-paired colloidal gold immunochromatographic test strip (Sa-GICS). The detection limit achieved using the Sa-GICS approach was 10(5) CFU/mL, with a result that can be observed by the naked eye within 10 min. Moreover, Sa-GICS can detect eight strains of Aac and display no cross-reactions with other pathogenic plant microorganisms. Artificial contamination experiments demonstrated that Sa-GICS would not be affected by impurities in the leaves or stems of the plants and were consistent with the PCR results. This is the first report on the development of a colloidal gold immunoassay strip with self-paired single McAb for the rapid detection of Aac. Graphical Abstract Schematic representation of the test strip.
authors.affiliation: School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.
authors.forename: Haijuan
authors.initials: H
authors.lastname: Zeng
authors.affiliation: Institute of Agri-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai, 201403, China.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Guo
authors.affiliation: Shanghai University of Medicine and Health Sciences, Shanghai, 200093, China.
authors.forename: Beibei
authors.initials: B
authors.lastname: Liang
authors.affiliation: School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.
authors.forename: Jianwu
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.
authors.forename: Xuzhao
authors.initials: X
authors.lastname: Zhai
authors.affiliation: School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Song
authors.affiliation: Chinese Academy of Inspection and Quarantine, Beijing, 100029, China. wenjunzhao@188.com.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Institute of Biochemistry and Molecular Biology, ZBMZ, University of Freiburg, 79104, Freiburg, Germany. enguo.fan@biochemie.uni-freiburg.de.
authors.forename: Enguo
authors.initials: E
authors.lastname: Fan
authors.affiliation: School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China. liuq@usst.edu.cn.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D042621:Comamonadaceae / Q000302:isolation & purification*; D028464:Cucurbita / Q000382:microbiology*; D004867:Equipment Design; D017979:Gold Colloid / Q000737:chemistry; D057230:Limit of Detection; D010935:Plant Diseases / Q000382:microbiology*; D011934:Reagent Strips / Q000032:analysis*
chemical_list: D000911:Antibodies, Monoclonal; D017979:Gold Colloid; D011934:Reagent Strips
keywords: Acidovorax avenae subsp. citrulli; Colloidal gold; Immunoassay; Monoclonal antibody; Self-paired; Test strip