title: Nanocomposite Membranes Enhance Bone Regeneration Through Restoring Physiological Electric Microenvironment.
abstract: Physiological electric potential is well-known for its indispensable role in maintaining bone volume and quality. Although implanted biomaterials simulating structural, morphological, mechanical, and chemical properties of natural tissue or organ has been introduced in the field of bone regeneration, the concept of restoring physiological electric microenvironment remains ignored in biomaterials design. In this work, a flexible nanocomposite membrane mimicking the endogenous electric potential is fabricated to explore its bone defect repair efficiency. BaTiO3 nanoparticles (BTO NPs) were first coated with polydopamine. Then the composite membranes are fabricated with homogeneous distribution of Dopa@BTO NPs in poly(vinylidene fluoridetrifluoroethylene) (P(VDF-TrFE)) matrix. The surface potential of the nanocomposite membranes could be tuned up to -76.8 mV by optimizing the composition ratio and corona poling treatment, which conform to the level of endogenous biopotential. Remarkably, the surface potential of polarized nanocomposite membranes exhibited a dramatic stability with more than half of original surface potential remained up to 12 weeks in the condition of bone defect. In vitro, the membranes encouraged bone marrow mesenchymal stem cells (BM-MSCs) activity and osteogenic differentiation. In vivo, the membranes sustainably maintained the electric microenvironment giving rise to rapid bone regeneration and complete mature bone-structure formation. Our findings evidence that physiological electric potential repair should be paid sufficient attention in biomaterials design, and this concept might provide an innovative and well-suited strategy for bone regenerative therapies.
authors.affiliation: Department of Dental Materials, Peking University School and Hospital of Stomatology , Beijing 100081, PR China.|National Engineering Laboratory for Digital and Material Technology of Stomatology , Beijing 100081, PR China.|Beijing Laboratory of Biomedical Materials, Peking University School and Hospital of Stomatology , Beijing 100081, PR China.
authors.forename: Xuehui
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan, Hubei 430022, PR China.
authors.forename: Chenguang
authors.initials: C
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of New Ceramics and Fine Processing, and Department of Materials Science and Engineering, Tsinghua University , Beijing 100084, PR China.
authors.forename: Yuanhua
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Department of Polymer Science & Engineering, School of Chemistry & Biological Engineering, University of Science & Technology Beijing , Beijing 100083, China.
authors.forename: Penghao
authors.initials: P
authors.lastname: Hu
authors.affiliation: State Key Laboratory of New Ceramics and Fine Processing, and Department of Materials Science and Engineering, Tsinghua University , Beijing 100084, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Shen
authors.affiliation: State Key Laboratory of New Ceramics and Fine Processing, and Department of Materials Science and Engineering, Tsinghua University , Beijing 100084, PR China.
authors.forename: Ke
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Geriatric Dentistry, Peking University School and Hospital of Stomatology , Beijing 100081, PR China.
authors.forename: Song
authors.initials: S
authors.lastname: Meng
authors.affiliation: Department of Dental Materials, Peking University School and Hospital of Stomatology , Beijing 100081, PR China.|National Engineering Laboratory for Digital and Material Technology of Stomatology , Beijing 100081, PR China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Chai
authors.affiliation: Department of Geriatric Dentistry, Peking University School and Hospital of Stomatology , Beijing 100081, PR China.
authors.forename: Xiaohan
authors.initials: X
authors.lastname: Dai
authors.affiliation: Department of Geriatric Dentistry, Peking University School and Hospital of Stomatology , Beijing 100081, PR China.
authors.forename: Xing
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Geriatric Dentistry, Peking University School and Hospital of Stomatology , Beijing 100081, PR China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Geriatric Dentistry, Peking University School and Hospital of Stomatology , Beijing 100081, PR China.
authors.forename: Xiaoju
authors.initials: X
authors.lastname: Mo
authors.affiliation: Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan, Hubei 430022, PR China.
authors.forename: Cen
authors.initials: C
authors.lastname: Cao
authors.affiliation: Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan, Hubei 430022, PR China.
authors.forename: Shue
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Dental Materials, Peking University School and Hospital of Stomatology , Beijing 100081, PR China.|Department of Geriatric Dentistry, Peking University School and Hospital of Stomatology , Beijing 100081, PR China.|National Engineering Laboratory for Digital and Material Technology of Stomatology , Beijing 100081, PR China.|Beijing Laboratory of Biomedical Materials, Peking University School and Hospital of Stomatology , Beijing 100081, PR China.
authors.forename: Xuliang
authors.initials: X
authors.lastname: Deng
authors.affiliation: Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan, Hubei 430022, PR China.
authors.forename: Lili
authors.initials: L
authors.lastname: Chen
keywords: bone regeneration; ferroelectricity; nanocomposite membranes; physiological electric potential; polarization