title: MicroRNA-145 functions as a tumor suppressor by targeting matrix metalloproteinase 11 and Rab GTPase family 27a in triple-negative breast cancer.
abstract: Although increasing evidence has documented that microRNA-145 (miR-145) acts as a tumor suppressor in breast cancer, its exact role in triple-negative breast cancer (TNBC) remains poorly defined. In this study, the expression of miR-145 in human TNBC cells and samples from 30 patients was analyzed by stem-loop real-time PCR. We found that miR-145 was significantly downregulated in TNBC tissues and cells. Upregulating miR-145 in HCC1937 cells dramatically suppressed cell proliferation and induced G1-phase arrest, whereas MDA-MB-231 cells did not show growth inhibition. MiR-145 exhibited an inhibitory role in cell invasion through the post-transcriptional regulation of the novel targets MMP11 and Rab27a in TNBC cells. Additionally, miR-145 silencing could be reversed by 5-aza-2'-deoxycytidine (DAC). These results demonstrated that miR-145 has an inhibitory role in TNBC malignancy by targeting MMP11 and Rab27a, which might be potential therapeutic and diagnostic targets for TNBC.
authors.affiliation: Department of Clinical Laboratory, Nanjing Medical University Cancer Hospital, Nanjing, PR China.
authors.forename: L
authors.initials: L
authors.lastname: Tang
authors.affiliation: Department of Surgery, Nanjing Medical University Cancer Hospital, Nanjing, PR China.
authors.forename: D
authors.initials: D
authors.lastname: Wei
authors.affiliation: Department of Clinical Laboratory, Nanjing Medical University Cancer Hospital, Nanjing, PR China.
authors.forename: F
authors.initials: F
authors.lastname: Yan
mesh_terms: D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D019175:DNA Methylation; D015536:Down-Regulation; D000066630:Ectopic Gene Expression; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D006801:Humans; D053507:Matrix Metalloproteinase 11 / Q000235:genetics* / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics*; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D064726:Triple Negative Breast Neoplasms / Q000235:genetics* / Q000378:metabolism; D000075745:rab27 GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: C541010:MIRN145 microRNA, human; D035683:MicroRNAs; D000075745:rab27 GTP-Binding Proteins; D053507:Matrix Metalloproteinase 11; C066694:RAB27A protein, human