title: Characterization of iron-sulfur clusters in flavin-containing opine dehydrogenase.
abstract: Flavin-containing opine dehydrogenase from Bradyrhizobium japonicum forms a heterooligomeric α4β4γ4enzyme complex. An electron paramagnetic resonance spectroscopy analysis using wild-type and site-directed mutants revealed that [4Fe-4S] and [2Fe-2S] clusters bind to two different types of [Fe-S] binding sites in the γ- and α-subunits, respectively. The latter was found to be important for structural folding and enzyme catalysis.
authors.affiliation: a Department of Bioscience , Graduate School of Agriculture, Ehime University , Matsuyama , Japan.|b Center for Marine Environmental Studies , Ehime University , Matsuyama , Japan.
authors.forename: Seiya
authors.initials: S
authors.lastname: Watanabe
authors.affiliation: c Department of Bio-molecular Engineering, Graduate School of Science and Technology , Kyoto Institute of Technology , Kyoto , Japan.
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Tajima
authors.affiliation: c Department of Bio-molecular Engineering, Graduate School of Science and Technology , Kyoto Institute of Technology , Kyoto , Japan.
authors.forename: Kazuma
authors.initials: K
authors.lastname: Matsui
authors.affiliation: a Department of Bioscience , Graduate School of Agriculture, Ehime University , Matsuyama , Japan.
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Watanabe
mesh_terms: D000595:Amino Acid Sequence; D055162:Biocatalysis; D020369:Bradyrhizobium / Q000201:enzymology*; D005415:Flavins / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D010088:Oxidoreductases / Q000737:chemistry* / Q000378:metabolism*; D013455:Sulfur / Q000378:metabolism*
chemical_list: D005415:Flavins; D013455:Sulfur; D007501:Iron; D010088:Oxidoreductases
keywords: [2Fe-2S] cluster; [4Fe-4S] cluster; electron spin resonance spectroscopy; opine dehydrogenase