title: Vitamin D prevents glycation of proteins: an in vitro study.
abstract: Human serum albumin (HSA) is an important protein involved in the transport of hormones, fatty acids, drugs, and other macromolecules. Under hyperglycemic conditions, this molecule undergoes irreversible modification that affects its structure and function. In this study, we explored the effect of two forms of vitamin D, a nutraceutical, on glycation modification in HSA. The protein was incubated with a physiologically high concentration of glucose in the presence of vitamin D metabolites. After 21 days, samples were tested for secondary structural changes, side chain modification, and the presence of advanced glycation end products. Vitamin D metabolites could reduce glycation modification, albeit only to a small extent. Interaction studies reveal that Vitamin D interaction with HSA can prevent protein glycation.
authors.affiliation: Department of Biochemistry, Aligarh Muslim University, India.
authors.forename: Sarah
authors.initials: S
authors.lastname: Iqbal
authors.affiliation: Department of Biochemistry, Aligarh Muslim University, India.
authors.forename: Md Maroof
authors.initials: MM
authors.lastname: Alam
authors.affiliation: Department of Biochemistry, Aligarh Muslim University, India.
authors.forename: Imrana
authors.initials: I
authors.lastname: Naseem
mesh_terms: D019587:Dietary Supplements / Q000032:analysis; D005947:Glucose / Q000378:metabolism*; D017127:Glycation End Products, Advanced / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D006943:Hyperglycemia / Q000378:metabolism* / Q000473:pathology; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism*; D014807:Vitamin D / Q000378:metabolism*
chemical_list: D017127:Glycation End Products, Advanced; D012709:Serum Albumin; D014807:Vitamin D; D005947:Glucose
keywords: calcitriol; glycation; protein