title: Isolation of a new β-carboline alkaloid from aerial parts of Triclisia sacleuxii and its antibacterial and cytotoxicity effects.
abstract: A new β-carboline alkaloid named sacleuximine A (1) together with known compounds palmatine (2), isotetrandrine (3), trans-N-feruloyltyramine (4), trans-N-caffeoyltyramine (5), yangambin (6), syringaresinol (7), sesamin (8), (+) epi-quercitol (9), 4-hydroxybenzaldehyde (10), β-sitosterol (11), quercetin 3-O-rutinoside (12) and myricetin 3-O-β-glucose (1→6) α-rhamnoside (13) have been isolated from methanol extract of Triclisia sacleuxii aerial parts. Compounds 1-10 were evaluated for their cytotoxicity against human adenocarcinoma (HeLa), human hepatocarcinoma (Hep3B) and human breast carcinoma (MCF-7) cells lines and also for antibacterial activities against both Gram-positive and Gram-negative bacteria. The cytotoxicity (IC50) values ranged between 0.15 and 36.7 μM while the minimum inhibitory concentrations were found to be in the range of 3.9 and 125 μM, respectively. This is the first report of antibacterial compounds and the isolation of lignans together with a β-carboline alkaloid from T. sacleuxii.
authors.affiliation: a Department of Chemistry, School of Physical and Biological Sciences , Maseno University , Maseno , Kenya.
authors.forename: Fidelis
authors.initials: F
authors.lastname: Samita
authors.affiliation: a Department of Chemistry, School of Physical and Biological Sciences , Maseno University , Maseno , Kenya.
authors.forename: Charles Otieno
authors.initials: CO
authors.lastname: Ochieng
authors.affiliation: a Department of Chemistry, School of Physical and Biological Sciences , Maseno University , Maseno , Kenya.
authors.forename: Philip Okinda
authors.initials: PO
authors.lastname: Owuor
authors.affiliation: a Department of Chemistry, School of Physical and Biological Sciences , Maseno University , Maseno , Kenya.
authors.forename: Lawrence Onyango Arot
authors.initials: LO
authors.lastname: Manguro
authors.affiliation: b Department of Chemistry, School of Biological and Physical Sciences , University of Nairobi , Nairobi , Kenya.
authors.forename: Jacob Ogweno
authors.initials: JO
authors.lastname: Midiwo
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002243:Carbolines / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D045744:Cell Line, Tumor; D006801:Humans; D029583:Menispermaceae / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*
chemical_list: D000470:Alkaloids; D000900:Anti-Bacterial Agents; D000972:Antineoplastic Agents, Phytogenic; D002243:Carbolines; D010936:Plant Extracts
keywords: Menispermaceae; Triclisia sacleuxii; aerial parts; antibacterial; cytotoxicity; secondary metabolites