title: Saccharification of microalgae biomass obtained from wastewater treatment by enzymatic hydrolysis. Effect of alkaline-peroxide pretreatment.
abstract: An enzymatic method for the carbohydrate hydrolysis of different microalgae biomass cultivated in domestic (DWB) and pig manure (PMWB) wastewaters, at different storage conditions (fresh, freeze-dried and reconstituted), was evaluated. The DWB provided sugars yields between 40 and 63%, although low xylose yields (< 23.5%). Approximately 2% of this biomass was converted to byproducts as succinic, acetic and formic acids. For PMWB, a high fraction of the sugars (up to 87%) was extracted, but mainly converted into acetic, butyric and formic acids, which was attributed to the bacterial action. In addition, the performance of an alkaline-peroxide pretreatment, conducted for 1h, 50°C and H2O2 concentrations from 1 to 7.5% (w/w), was essayed. The hydrolysis of pretreated microalgae supported a wide range of sugars extraction for DWB (55-90%), and 100% for PMWB. Nevertheless, a large fraction of these sugars (∼30% for DWB and 100% for PMWB) was transformed to byproducts.
authors.affiliation: Department of Chemical Engineering and Environmental Technology, University of Valladolid, Calle Doctor Mergelina s/n, 47011 Valladolid, Spain.
authors.forename: Judit
authors.initials: J
authors.lastname: Martín Juárez
authors.affiliation: Department of Chemical Engineering and Environmental Technology, University of Valladolid, Calle Doctor Mergelina s/n, 47011 Valladolid, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Lorenzo Hernando
authors.affiliation: Department of Chemical Engineering and Environmental Technology, University of Valladolid, Calle Doctor Mergelina s/n, 47011 Valladolid, Spain.
authors.forename: Raúl
authors.initials: R
authors.lastname: Muñoz Torre
authors.affiliation: The Institute of the Environment, La Serna, 58, 24007 León, Spain.
authors.forename: Saúl
authors.initials: S
authors.lastname: Blanco Lanza
authors.affiliation: Department of Chemical Engineering and Environmental Technology, University of Valladolid, Calle Doctor Mergelina s/n, 47011 Valladolid, Spain. Electronic address: silvia@iq.uva.es.
authors.forename: Silvia
authors.initials: S
authors.lastname: Bolado Rodríguez
mesh_terms: D019342:Acetic Acid / Q000737:chemistry; D000818:Animals; D018533:Biomass; D020148:Butyric Acid / Q000737:chemistry; D002241:Carbohydrates / Q000737:chemistry; D005561:Formates / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000737:chemistry*; D006868:Hydrolysis; D058086:Microalgae / Q000737:chemistry / Q000378:metabolism*; D013552:Swine; D018505:Waste Management / Q000379:methods*; D062065:Wastewater; D014994:Xylose / Q000737:chemistry
chemical_list: D019342:Acetic Acid; D020148:Butyric Acid; D002241:Carbohydrates; D005561:Formates; D006861:Hydrogen Peroxide; D062065:Wastewater; D014994:Xylose; C030544:formic acid
keywords: Alkaline-peroxide pretreatment; Enzymatic hydrolysis; Glucose; Wastewater; Xylose