title: Optimization & design of isradipine loaded solid lipid nanobioparticles using rutin by Taguchi methodology.
abstract: Our research objective was to optimize and design nano-biosystem of Isradipine (IDP) via novel bioenhancer (Rutin) loaded solid-lipid nanobioparticles (ANbp) using Taguchi design (TgD) methodology. Firstly, preliminary screening of solid lipid nanoparticles (SLNps) formulation core factors (A, B & C; Lipoid's, poly-acid, sonication time respectively at fixed dose of model drug were assessed on entrapment efficiency & particle size; R1& R2) by performed experimentally of three factor three levels orthogonal L27array. Consequently, signal to noise (S/N) ratio plot of responses were drawn to predict better quality fitted-levels of significant factor for eminence optimization. Further, optimized quality spaces composition was used via enhancer (Rutin) to design advanced bio-formulation (ANbp) and done its evaluation (entrapment efficiency, particle size, drug release & kinetics). As designed, ANbp results showed better sustained (86.54% as compared to control SLNps 94.48% in 24h) release, kinetics & stability behavior with good entrapment efficiency (97.58%) and desired smaller particle size (108nm). Therefore, statistically (TgD) optimization strategy would be considered to design nano-drug delivery system with bio-agent in-order to improve oral bioavailability of antihypertensive agents.
authors.affiliation: Department of RIC, I.K. Gujral Punjab Technical University, Kapurthala, Punjab, India. Electronic address: vikashruhilo1@gmail.com.
authors.forename: Vikash
authors.initials: V
authors.lastname: Kumar
authors.affiliation: CT Institute of Pharmaceutical Sciences, Jalandhar, Punjab, India. Electronic address: rajeevkharb@gmail.com.
authors.forename: Rajeev
authors.initials: R
authors.lastname: Kharb
authors.affiliation: P. D. M College of Pharmacy, B'garh, India.
authors.forename: Hema
authors.initials: H
authors.lastname: Chaudhary
mesh_terms: D065546:Drug Liberation; D017275:Isradipine / Q000494:pharmacology*; D007700:Kinetics; D008055:Lipids / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D012431:Rutin / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D008055:Lipids; D012431:Rutin; D017275:Isradipine
keywords: Design; Drug delivery; Isradipine; Nanobioparticles; Optimization; Rutin