title: Sonic hedgehog, TBX18, and TSHZ3 proteins involved in pyeloureteral motility development are overexpressed in ureteropelvic junction obstruction. An immunohistochemical, histopathological, and clinical comparative study.
abstract: OBJECTIVE
  To compare pathological samples obtained from cases that underwent surgery for ureteropelvic junction (UPJ) obstruction with samples obtained during autopsies of subjects.
  
  
  METHODS
  Retrospectively, 42 patients who had undergone surgery due to UPJ obstruction (group 1) were included in the study. Histopathological and immunohistochemical features for sonic hedgehog (SHH), TBX18, and TSHZ3 of UPJ were evaluated and findings were compared with 20 autopsy cases (group 2).
  
  
  RESULTS
  In group 1, the scores were statistically significantly higher in terms of cytoplasmic SHH, nuclear TBX18, cytoplasmic and nuclear TSHZ3 staining. Statistically, no correlation was found between age and the staining scores belonging to these 3 antibodies in group 1 and group 2. Intense inflammation was found to be related with nuclear staining for TBX18.
  
  
  CONCLUSIONS
  Gene product expressions of SHH, TBX18 and TSHZ3 are statistically higher in patients with UPJ obstruction, when compared with control group. The explanation may be the reactivation of the processes, which had shown their effects in the embryological period, due to the chronic inflammation and long-term micro-trauma created by the disease.
authors.affiliation: Department of Pediatric Surgery, Faculty of Medicine, Celal Bayar University, Manisa, Turkey. E-mail. omeryilmaz71@gmail.com.
authors.forename: Omer
authors.initials: O
authors.lastname: Yilmaz
authors.forename: Nalan
authors.initials: N
authors.lastname: Nese
authors.forename: Mustafa
authors.initials: M
authors.lastname: Dalgic
authors.forename: Gonca P
authors.initials: GP
authors.lastname: Kesici
authors.forename: Abdulkadir
authors.initials: A
authors.lastname: Genc
authors.forename: Can
authors.initials: C
authors.lastname: Taneli
authors.forename: Cuneyt
authors.initials: C
authors.lastname: Gunsar
authors.forename: Aydın
authors.initials: A
authors.lastname: Sencan
authors.forename: Hasan
authors.initials: H
authors.lastname: Cayirli
authors.forename: Aydın
authors.initials: A
authors.lastname: Isisag
mesh_terms: D053823:Hedgehog Proteins / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D020825:T-Box Domain Proteins / Q000378:metabolism*; D014517:Ureteral Obstruction / Q000378:metabolism*
chemical_list: D053823:Hedgehog Proteins; D018398:Homeodomain Proteins; C509239:SHH protein, human; D020825:T-Box Domain Proteins; C580600:TSHZ3 protein, human; C523796:Tbx18 protein, human