title: p66Shc: A novel biomarker of tubular oxidative injury in patients with diabetic nephropathy.
abstract: Increased p66Shc expression has been associated with diabetic nephropathy (DN). However, whether p66Shc can serve as a potential biomarker for tubular oxidative injury in DN is unknown. We measured the expression of p66Shc in peripheral blood monocytes (PBMs) and renal biopsy tissues from DN patients and then analysed the relationship between p66Shc expression and the clinical characteristics of patients with DN. Patients were divided into 4 groups (class IIa, class IIb, class III and the control group). qPCR, Western blotting and immunohistochemistry were performed. The results showed that both p66Shc and p-p66Shc expression significantly increased in PBMs and kidney tissues of DN patients. Moreover, Spearman's correlation and multiple regression analyses were carried out. A positive relationship between the p66Shc expression and oxidative stress was found. p66Shc and oxidative stress were significant predictors of the degree of tubular damage. In addition, p66Shc expression was positively correlated with the concentrations of β-NAG, UACR and 8-OHdG, low-density lipoprotein and blood glucose levels, and duration of diabetes in patients with DN from class IIa to class III. These data indicated that increased expression of p66Shc may serve as a therapeutic target and a novel biomarker of DN.
authors.affiliation: Department of Nephrology, 2nd Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Xiaoxuan
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Nephrology, 2nd Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Xuejing
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Department of Nephrology, 2nd Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Mingming
authors.initials: M
authors.lastname: Ma
authors.affiliation: Department of Nephrology, 2nd Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Yachun
authors.initials: Y
authors.lastname: Han
authors.affiliation: Department of Nephrology, 2nd Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Hu
authors.affiliation: Department of Nephrology, 2nd Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Shuguang
authors.initials: S
authors.lastname: Yuan
authors.affiliation: Department of Nephrology, 2nd Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Nephrology, 2nd Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xiao
authors.affiliation: Department of Nephrology, 2nd Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Fuyou
authors.initials: F
authors.lastname: Liu
authors.affiliation: Departments of Pathology &Medicine, Northwestern University, Chicago, USA.
authors.forename: Yashpal S
authors.initials: YS
authors.lastname: Kanwar
authors.affiliation: Department of Nephrology, 2nd Xiangya Hospital, Central South University, Changsha, Hunan, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Sun
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis*; D001706:Biopsy; D001773:Blood Cells / Q000737:chemistry; D015153:Blotting, Western; D003928:Diabetic Nephropathies / Q000473:pathology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007668:Kidney / Q000473:pathology; D007963:Leukocytes, Mononuclear / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D000071425:Src Homology 2 Domain-Containing, Transforming Protein 1 / Q000032:analysis*
chemical_list: D015415:Biomarkers; C528024:SHC1 protein, human; D000071425:Src Homology 2 Domain-Containing, Transforming Protein 1