title: Amycolatopsis albispora sp. nov., isolated from deep-sea sediment.
abstract: A novel Gram-stain-positive, aerobic, non-motile actinobacterium, designated strain WP1T, was isolated from a deep-sea sediment sample collected from the Indian Ocean, and examined in a taxonomic study using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain WP1T formed a distinct phyletic lineage in the genus Amycolatopsis and was closely related to A. magusensis KT2025T (97.8 % 16S rRNA gene sequence similarity), A. palatopharyngis 1BDZT (97.3 %) and A. marinaMs392AT (97.2 %). The isolate grew at 4-45 °C, pH 5-11 and in the presence of 0-8 % (w/v) NaCl. The cell wall of the novel strain contained meso-diaminopimelic acid and arabinose and galactose as the diagnostic sugars. Major fatty acids identified were iso-C16 : 0, C17 : 1ω8c and C17 : 1ω6c. The predominant menaquinones were MK-9(H4) and MK-7. The polar lipids detected were phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylmethylethanolamine, four unknown phospholipids and one unknown glycolipid. The genomic DNA G+C content was 72.2 mol%. Strain WP1T displayed low DNA-DNA reassociation with A. magusensis DSM 45510T (mean value 36.2 %), A. palatopharyngis JCM 12460T (31.5 %) and A. marina JCM 16121T (29.7 %). Based on the data reported here, strain WP1T represents a novel species within the genus Amycolatopsis, for which the name Amycolatopsis albisporasp. nov. is proposed. The type strain is WP1T (=KCTC 39642T=MCCC 1A10745T).
authors.affiliation: Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, Fujian, PR China.|South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Guangzhou, PR China.
authors.forename: Gaiyun
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, Fujian, PR China.|School of Biological Engineering, Qilu University of Technology, Jinan 250353, Shandong, PR China.
authors.forename: Lina
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of Biological Engineering, Qilu University of Technology, Jinan 250353, Shandong, PR China.
authors.forename: Jinglong
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, Fujian, PR China.|South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Guangzhou, PR China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology*; D006017:Glycolipids / Q000737:chemistry; D019156:Indian Ocean; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006017:Glycolipids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9; D003960:Diaminopimelic Acid