title: Molecular characterization of the IgE-binding epitopes in the fast ω-gliadins of Triticeae in relation to wheat-dependent, exercise-induced anaphylaxis.
abstract: Fast ω-gliadins were minor components of wheat storage proteins but a major antigen triggering allergy to wheat. Sixty-six novel full-length fast ω-gliadin genes with unique characteristics were cloned and sequenced from wheat and its relative species using a PCR-based strategy. Their coding regions ranged from 177bp to 987bp in length and encoded 4.28kDa to 37.56kDa proteins. On the base of first three deduced amino acids at the N-terminal, these genes could be classified into the six subclasses of SRL-, TRQ-, GRL-, NRL-, SRP- and SRM-type ω-gliadin genes. Compared by multiple alignments, these genes were significantly different from each other, due to the insertion or deletion at the repetitive domain. An analysis of the IgE-binding epitopes of the 66 deduced fast ω-gliadins demonstrated that they contained 0-24 IgE-binding epitopes. The phylogenetic tree demonstrated that the fast ω-gliadins and slow ω-gliadins were separated into two groups and their divergence time was 21.64millionyears ago. Sequence data of the fast ω-gliadin genes assist in the study of the origins and evolutions of the different types of ω-gliadins while also providing a basis for the synthesis of monoclonal antibodies to detect wheat antigen content.
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai'an 271018, Shandong, PR China; College of Food Science, Shandong Agricultural University, No. 61, Daizong Street, Tai'an 271018, Shandong, PR China.
authors.forename: Xuye
authors.initials: X
authors.lastname: Du
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai'an 271018, Shandong, PR China.
authors.forename: Heng
authors.initials: H
authors.lastname: Tang
authors.affiliation: Heze University, No. 2269, Daxue Street, Heze 274015, Shandong, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai'an 271018, Shandong, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai'an 271018, Shandong, PR China.
authors.forename: Huayan
authors.initials: H
authors.lastname: Yin
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai'an 271018, Shandong, PR China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Food Science, Shandong Agricultural University, No. 61, Daizong Street, Tai'an 271018, Shandong, PR China.
authors.forename: Xiaocun
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Food Science, Shandong Agricultural University, No. 61, Daizong Street, Tai'an 271018, Shandong, PR China.
authors.forename: Xuguang
authors.initials: X
authors.lastname: Qiao
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai'an 271018, Shandong, PR China.
authors.forename: Anfei
authors.initials: A
authors.lastname: Li
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai'an 271018, Shandong, PR China. Electronic address: lkong@sdau.edu.cn.
authors.forename: Lingrang
authors.initials: L
authors.lastname: Kong
mesh_terms: D000595:Amino Acid Sequence; D000707:Anaphylaxis / Q000209:etiology* / Q000276:immunology*; D003001:Cloning, Molecular; D000939:Epitopes / Q000235:genetics*; D015444:Exercise; D017343:Genes, Plant; D005903:Gliadin / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology; D007073:Immunoglobulin E / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D014908:Triticum / Q000235:genetics*
chemical_list: D000939:Epitopes; D007073:Immunoglobulin E; D005903:Gliadin
keywords: Allergy; Fast ω-gliadin; IgE-binding epitopes; Triticum aestivum; Variation