title: (1)H, (13)C and (15)N backbone resonance assignments and dynamic properties of the PDZ tandem of Whirlin.
abstract: Mammals perceive sounds thanks to mechanosensory hair cells located in the inner ear. The stereocilia of these cells are tightly bound together in bundles by a network of cadherins and scaffolding proteins. Stereocilia deflection induces stretching of this network and is responsible for hair cell depolarization that triggers the neuronal message, transducing the mechanical signal into an electric signal transmissible to the brain. Nearly all proteins involved in this mechano-electrical transduction network contain short C-terminal motifs of interaction with PDZ domains (PSD-95, Discs Large, ZO-1). Interestingly only two of these proteins encompass PDZ domains: Harmonin and Whirlin. As our first step towards a comprehensive structural study of Whirlin, we have assigned the (1)H, (13)C and (15)N backbone resonances of a tandem formed by the first two PDZ domains of Whirlin, reported the secondary structure elements of this tandem as predicted by the TALOS+ server and evaluated its dynamics from (15)N relaxation measurements.
authors.affiliation: Unité de Résonance Magnétique Nucléaire des Biomolécules, Département de Biologie Structurale et Chimie, Institut Pasteur, 28, rue du Docteur Roux, 75015, Paris, France.|CNRS, UMR3528, 75015, Paris, France.|Cellule Pasteur UPMC, Univ. Pierre et Marie Curie, rue du Dr Roux, 75015, Paris, France.
authors.forename: Florent
authors.initials: F
authors.lastname: Delhommel
authors.affiliation: Unité de Résonance Magnétique Nucléaire des Biomolécules, Département de Biologie Structurale et Chimie, Institut Pasteur, 28, rue du Docteur Roux, 75015, Paris, France.|CNRS, UMR3528, 75015, Paris, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Wolff
authors.affiliation: Unité de Résonance Magnétique Nucléaire des Biomolécules, Département de Biologie Structurale et Chimie, Institut Pasteur, 28, rue du Docteur Roux, 75015, Paris, France. florence.cordier@pasteur.fr.|CNRS, UMR3528, 75015, Paris, France. florence.cordier@pasteur.fr.
authors.forename: Florence
authors.identifier: 0000-0002-5963-7773
authors.initials: F
authors.lastname: Cordier
mesh_terms: D000818:Animals; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism*; D051379:Mice; D019906:Nuclear Magnetic Resonance, Biomolecular; D054731:PDZ Domains; D017433:Protein Structure, Secondary
chemical_list: D008565:Membrane Proteins; C477218:Whrn protein, mouse
keywords: Backbone resonance assignments; Mechano-electrical transduction; PDZ; PDZ tandem; Whirlin