title: Asiatic Acid Inhibits Lipopolysaccharide-Induced Acute Lung Injury in Mice.
abstract: Asiatic acid (AA), a major triterpene isolated from Centella asiatica (L.) Urban, is known to exert various pharmacological activities, including anti-inflammatory and antioxidant effects. The aim of this study was to evaluate the anti-inflammatory effects of AA on lipopolysaccharide (LPS)-induced acute lung injury (ALI) and clarify the underlying mechanisms. Lung pathological changes were assessed by H&E staining. The myeloperoxidase (MPO) activity was detected by MPO assay. The levels of inflammatory cytokines were measured by ELISA. TLR4 and NF-kB expression was detected by Western blot analysis. AA obviously inhibited LPS-induced lung histopathological changes, MPO activity, and inflammatory cell numbers in bronchoalveolar lavage fluid (BALF). Treatment of AA also inhibited LPS-induced TNF-α, IL-6, and IL-1β production. Furthermore, Western blot analysis showed that AA inhibited LPS-induced TLR4 expression and NF-kB activation. In conclusion, AA inhibited LPS-induced ALI in mice by inhibiting inflammatory cytokine production, which is mediated via blocking of the TLR4/NF-kB signaling pathway.
authors.affiliation: Translational Medicine Center of Sepsis, The Third Xiangya Hospital, Central South University, Changsha, Hunan, 410013, People's Republic of China.|Department of Critical Care Medicine, The Third Xiangya Hospital, Central South University, Changsha, Hunan, 410013, People's Republic of China.|Laboratory of Shock, Department of Pathophysiology, Xiangya School of Medicine, Central South University, 110 Xiangya Road, Changsha, Hunan, 410078, People's Republic of China.
authors.forename: Zhiling
authors.initials: Z
authors.lastname: Li
authors.affiliation: Translational Medicine Center of Sepsis, The Third Xiangya Hospital, Central South University, Changsha, Hunan, 410013, People's Republic of China. xiaoxianzhong36@sina.com.|Department of Critical Care Medicine, The Third Xiangya Hospital, Central South University, Changsha, Hunan, 410013, People's Republic of China. xiaoxianzhong36@sina.com.|Laboratory of Shock, Department of Pathophysiology, Xiangya School of Medicine, Central South University, 110 Xiangya Road, Changsha, Hunan, 410078, People's Republic of China. xiaoxianzhong36@sina.com.
authors.forename: Xianzhong
authors.initials: X
authors.lastname: Xiao
authors.affiliation: Translational Medicine Center of Sepsis, The Third Xiangya Hospital, Central South University, Changsha, Hunan, 410013, People's Republic of China.|Department of Critical Care Medicine, The Third Xiangya Hospital, Central South University, Changsha, Hunan, 410013, People's Republic of China.
authors.forename: Mingshi
authors.initials: M
authors.lastname: Yang
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000188:drug therapy*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D016207:Cytokines / Q000032:analysis / Q000187:drug effects; D008070:Lipopolysaccharides; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D053978:Pentacyclic Triterpenes / Q000494:pharmacology* / Q000627:therapeutic use; D051197:Toll-Like Receptor 4 / Q000037:antagonists & inhibitors
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D008070:Lipopolysaccharides; D016328:NF-kappa B; D053978:Pentacyclic Triterpenes; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; C017032:asiatic acid
keywords: LPS; NF-kB; acute lung injury; asiatic acid