title: Next-Generation Sequencing of an 88-Year-Old Specimen of the Poorly Known Species Liagora japonica (Nemaliales, Rhodophyta) Supports the Recognition of Otohimella gen. nov.
abstract: Liagora japonica is a red algal species distributed in temperate regions of Japan. This species has not been collected from its type locality on the Pacific coast of Japan since 1927 and seems to have become extinct in this area. For molecular characterization of L. japonica, we extracted DNA from the topotype material of L. japonica collected in 1927, analyzed seven genes using Illumina next-generation sequencing, and compared these data with sequences from modern samples of similar red algae collected from the Japan Sea coast of Japan. Both morphological and molecular data from modern samples and historical specimens (including the lectotype and topotype) suggest that the specimens from the Pacific and Japan Sea coasts of Japan should be treated as a single species, and that L. japonica is phylogenetically separated from the genus Liagora. Based on the phylogenetic results and examination of reproductive structures, we propose Otohimella japonica gen. et comb. nov., characterized morphologically by diffuse carposporophytes, undivided carposporangia, and involucral filaments initiated only from the cortical cell on the supporting cell.
authors.affiliation: Kobe University Research Center for Inland Sea, Awaji, Hyogo, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Suzuki
authors.affiliation: Center for Life Science Research, University of Yamanashi, Yamanashi, Japan.|National Institute of Polar Research, Midori-cho, Tachikawa-shi, Tokyo, Japan.|Transdisciplinary Research Integration Center, Toranomon, Minato-ku, Tokyo, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Segawa
authors.affiliation: Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Meguro-ku, Tokyo, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mori
authors.affiliation: National Institute of Polar Research, Midori-cho, Tachikawa-shi, Tokyo, Japan.|Transdisciplinary Research Integration Center, Toranomon, Minato-ku, Tokyo, Japan.
authors.forename: Ayumi
authors.initials: A
authors.lastname: Akiyoshi
authors.affiliation: Department of Natural Sciences, Faculty of Arts and Sciences, Komazawa University, Setagaya-ku, Tokyo, Japan.|Faculty of Chemical and Biological Sciences, Japan Women's University, Tokyo, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Ootsuki
authors.affiliation: Bioresource Sciences, Faculty of Agriculture, Kyushu University, Higashi-ku, Fukuoka, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Kurihara
authors.affiliation: Department of Biology, Graduate School of Science, Kobe University, Rokkodai, Nada-ku, Kobe, Japan.
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Sakayama
authors.affiliation: Department of Botany, National Museum of Nature and Science, Tsukuba, Ibaraki, Japan.
authors.forename: Taiju
authors.initials: T
authors.lastname: Kitayama
authors.affiliation: The Hokkaido University Museum, Hokkaido University, Sapporo, Hokkaido, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Abe
authors.affiliation: Department of Biological Sciences, Faculty of Science, Hokkaido University, Sapporo, Hokkaido, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kogame
authors.affiliation: Kobe University Research Center for Inland Sea, Awaji, Hyogo, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kawai
authors.affiliation: Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Bunkyo-ku, Tokyo, Japan.
authors.forename: Hisayoshi
authors.initials: H
authors.lastname: Nozaki
mesh_terms: D048028:DNA, Algal / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D020459:Genes, rRNA; D059014:High-Throughput Nucleotide Sequencing; D007564:Japan; D010140:Pacific Ocean; D010802:Phylogeny; D000461:Rhodophyta / Q000235:genetics*; D013048:Specimen Handling; D009626:Terminology as Topic
chemical_list: D048028:DNA, Algal; D004275:DNA, Ribosomal