title: Complete genome sequences of a putative new alphapartitivirus detected in Rosa spp.
abstract: A putative new alphapartitivirus was detected by next-generation sequencing (NGS) in Rosa spp. and identified as rose partitivirus isolate Phyllis Bide (RoPV-PB). The virus is bipartite with a dsRNA1 fragment (1937 bp) encoding a putative RdRp and a dsRNA2 fragment (1811 bp) encoding the putative CP subunit of the virus. dsRNA1 of RoPV-BP is closely related to Vicia faba partitivirus 1, with identities of 67 % and 72 % for the nucleotide (nt) and deduced amino acid (aa) sequences, respectively. In NGS analysis of RoPV-BP, coverage was uneven across both dsRNA fragments, with GC/AT content appearing to be a major determinant of depth of coverage.
authors.affiliation: Sidney Laboratory, Canadian Food Inspection Agency, 8801 East Saanich Road, North Saanich, BC, V8L 1H3, Canada.
authors.forename: James
authors.initials: J
authors.lastname: Phelan
authors.affiliation: Sidney Laboratory, Canadian Food Inspection Agency, 8801 East Saanich Road, North Saanich, BC, V8L 1H3, Canada. delano.james@inspection.gc.ca.
authors.forename: Delano
authors.initials: D
authors.lastname: James
mesh_terms: D016679:Genome, Viral; D021141:Nucleic Acid Amplification Techniques; D010935:Plant Diseases / Q000821:virology*; D010942:Plant Viruses / Q000235:genetics* / Q000302:isolation & purification*; D027826:Rosa / Q000821:virology*