title: Screening of freshwater and seawater microalgae strains in fully controlled photobioreactors for biodiesel production.
abstract: Strain selection is one of the primary hurdles facing cost-effective microalgal biodiesel production. Indeed, the strain used affects both upstream and downstream biodiesel production processes. This study presents a screening procedure that considers the most significant criteria in microalgal biodiesel production including TAG production and wet extraction and recovery of TAGs. Fourteen freshwater and seawater strains were investigated. Large variation was observed between the strains in all the screening criteria. The overall screening procedure ultimately led to the identification of Parachlorella kessleri UTEX2229 and Nannochloropsis gaditana CCMP527 as the best freshwater and seawater strains, respectively. They featured the largest areal TAG productivity equal to 2.7×10(-3) and 2.3×10(-3)kgm(-2)d(-1), respectively. These two strains also displayed encouraging cell fragility in a high pressure bead milling process with 69% and 98% cell disruption at 1750bar making them remarkable strains for TAG extraction in wet environment.
authors.affiliation: GEPEA, Université de Nantes, CNRS UMR6144, bd de l'Université, CRTT, BP 406, 44602 Saint-Nazaire Cedex, France; AZM Center for Biotechnology Research and Its Applications, Laboratory of Applied Biotechnology, Lebanese University, EDST, Tripoli, Lebanon; Department of Food Science and Technology, Faculty of Agricultural and Veterinary Medicine, Lebanese University, Dekweneh, Beirut, Lebanon.
authors.forename: A
authors.initials: A
authors.lastname: Taleb
authors.affiliation: GEPEA, Université de Nantes, CNRS UMR6144, bd de l'Université, CRTT, BP 406, 44602 Saint-Nazaire Cedex, France.
authors.forename: R
authors.initials: R
authors.lastname: Kandilian
authors.affiliation: GEPEA, Université de Nantes, CNRS UMR6144, bd de l'Université, CRTT, BP 406, 44602 Saint-Nazaire Cedex, France.
authors.forename: R
authors.initials: R
authors.lastname: Touchard
authors.affiliation: GEPEA, Université de Nantes, CNRS UMR6144, bd de l'Université, CRTT, BP 406, 44602 Saint-Nazaire Cedex, France.
authors.forename: V
authors.initials: V
authors.lastname: Montalescot
authors.affiliation: GEPEA, Université de Nantes, CNRS UMR6144, bd de l'Université, CRTT, BP 406, 44602 Saint-Nazaire Cedex, France.
authors.forename: T
authors.initials: T
authors.lastname: Rinaldi
authors.affiliation: AZM Center for Biotechnology Research and Its Applications, Laboratory of Applied Biotechnology, Lebanese University, EDST, Tripoli, Lebanon.
authors.forename: S
authors.initials: S
authors.lastname: Taha
authors.affiliation: Department of Food Science and Technology, Faculty of Agricultural and Veterinary Medicine, Lebanese University, Dekweneh, Beirut, Lebanon.
authors.forename: H
authors.initials: H
authors.lastname: Takache
authors.affiliation: GEPEA, Université de Nantes, CNRS UMR6144, bd de l'Université, CRTT, BP 406, 44602 Saint-Nazaire Cedex, France.
authors.forename: L
authors.initials: L
authors.lastname: Marchal
authors.affiliation: GEPEA, Université de Nantes, CNRS UMR6144, bd de l'Université, CRTT, BP 406, 44602 Saint-Nazaire Cedex, France.
authors.forename: J
authors.initials: J
authors.lastname: Legrand
authors.affiliation: GEPEA, Université de Nantes, CNRS UMR6144, bd de l'Université, CRTT, BP 406, 44602 Saint-Nazaire Cedex, France. Electronic address: jeremy.pruvost@univ-nantes.fr.
authors.forename: J
authors.initials: J
authors.lastname: Pruvost
mesh_terms: D056804:Biofuels; D018533:Biomass; D000460:Chlorophyta / Q000378:metabolism*; D005227:Fatty Acids / Q000737:chemistry; D005618:Fresh Water; D008055:Lipids / Q000737:chemistry; D058086:Microalgae / Q000378:metabolism*; D009584:Nitrogen / Q000737:chemistry; D058433:Photobioreactors; D011312:Pressure; D012623:Seawater; D058009:Stramenopiles / Q000378:metabolism*
chemical_list: D056804:Biofuels; D005227:Fatty Acids; D008055:Lipids; D009584:Nitrogen
keywords: Biodiesel; Lipids; Microalgae; Photobioreactors; Triacylglycerols