title: Evidence for the Participation of Acid-Sensing Ion Channels (ASICs) in the Antinociceptive Effect of Curcumin in a Formalin-Induced Orofacial Inflammatory Model.
abstract: Curcumin, a major bioactive component of turmeric, has diverse therapeutic effects such as anti-inflammatory, antioxidant, anticancer, and antinociceptive activities. The acid-sensing ion channels (ASICs), which can be activated by acute drops in the extracellular pH, play an important role in nociception. However, very little is known about the interaction between ASICs and curcumin in nociception of inflammation. In our study, we investigated whether the antinociceptive effects of curcumin are mediated via ASICs using an orofacial nociceptive model and in vitro western blotting, immunofluorescence, whole-cell patch-clamp recordings in the trigeminal system. Intraperitoneally administered curcumin at a dose of 50 mg/kg can reduce hyperalgesia in both the phases of a formalin-induced orofacial nociceptive model. Curcumin reduced the amplitude of ASICs currents in a dose-dependent manner in trigeminal ganglion (TG) neurons, and curcumin also reduced the protein quantity but did not change the distribution of ASICs in TG. Thus, our results indicate that curcumin can reduce formalin-induced ASICs activation and thus inhibit ASICs-mediated inflammatory pain hypersensitivity.
authors.affiliation: Department of Pharmacology, Guangdong Medical University, No. 1, Xincheng Road of Songshan Lake Science and Technology Industry Park, Dongguan, 523808, Guangdong, People's Republic of China.|Affiliated Hospital of Guangdong Medical University, Zhanjiang, 524023, Guangdong, People's Republic of China.
authors.forename: Yongfu
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Pharmacology, Guangdong Medical University, No. 1, Xincheng Road of Songshan Lake Science and Technology Industry Park, Dongguan, 523808, Guangdong, People's Republic of China.
authors.forename: Dongyun
authors.initials: D
authors.lastname: Qin
authors.affiliation: Department of Pharmacology, Guangdong Medical University, No. 1, Xincheng Road of Songshan Lake Science and Technology Industry Park, Dongguan, 523808, Guangdong, People's Republic of China.
authors.forename: Huiling
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Pharmacology, Guangdong Medical University, No. 1, Xincheng Road of Songshan Lake Science and Technology Industry Park, Dongguan, 523808, Guangdong, People's Republic of China. zoefu2014@126.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Fu
mesh_terms: D062565:Acid Sensing Ion Channels / Q000187:drug effects* / Q000378:metabolism; D000200:Action Potentials / Q000187:drug effects*; D000818:Animals; D003474:Curcumin / Q000494:pharmacology*; D004195:Disease Models, Animal; D005145:Face; D005557:Formaldehyde / Q000633:toxicity; D005727:Ganglia, Spinal / Q000166:cytology; D007249:Inflammation / Q000188:drug therapy*; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D059225:Nociception / Q000187:drug effects; D017207:Rats, Sprague-Dawley; D012668:Trigeminal Ganglion / Q000187:drug effects* / Q000378:metabolism
chemical_list: D062565:Acid Sensing Ion Channels; D005557:Formaldehyde; D003474:Curcumin
keywords: ASICs; Curcumin; Nociception; Trigeminal ganglion