title: FcLDP1, a Gene Encoding a Late Embryogenesis Abundant (LEA) Domain Protein, Responds to Brassinosteroids and Abscisic Acid during the Development of Fruits in Fragaria chiloensis.
abstract: White Chilean strawberries (Fragaria chiloensis) are non-climacteric fruits, with an exotic color and aroma. In order to discover genes involved in the development of these fruits, we identified a fragment of a gene encoding a late embryogenesis abundant domain protein, FcLDP1, that was expressed in early stages of fruit development, particularly in receptacles. Hormones play key roles in regulating the development of non-climacteric fruits. We show that the brassinosteroid content of the white strawberry varies during development. Additionally, FcLDP1 as well as the closest ortholog in the woodland strawberry, F. vesca (FvLDP1) possess multiple brassinosteroid, as well as abscisic acid (ABA) response motifs in the promoter region, consistent with the response of transiently expressed FcLDP1 promoter-GFP fusions to these hormones, and the rise in FcLDP1 transcript levels in white strawberry fruits treated with brassinosteroids or ABA. These findings suggest that both hormones regulate FcLDP1 expression during the development of white strawberries.
authors.affiliation: Centro de Biología Molecular Vegetal, Departamento de Biología, Facultad de Ciencias, Universidad de Chile Santiago, Chile.
authors.forename: Analía
authors.initials: A
authors.lastname: Espinoza
authors.affiliation: Facultad de Química y Biología, Universidad de Santiago de ChileSantiago, Chile; Centro para el Desarrollo de la Nanociencia y la Nanotecnología, Universidad de Santiago de ChileSantiago, Chile.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Contreras
authors.affiliation: Facultad de Química y Biología, Universidad de Santiago de ChileSantiago, Chile; Centro para el Desarrollo de la Nanociencia y la Nanotecnología, Universidad de Santiago de ChileSantiago, Chile.
authors.forename: Gustavo E
authors.initials: GE
authors.lastname: Zúñiga
authors.affiliation: Laboratorio de Fisiología Vegetal y Genética Molecular, Instituto de Ciencias Biológicas, Universidad de Talca Talca, Chile.
authors.forename: Raúl
authors.initials: R
authors.lastname: Herrera
authors.affiliation: Laboratorio de Fisiología Vegetal y Genética Molecular, Instituto de Ciencias Biológicas, Universidad de Talca Talca, Chile.
authors.forename: María Alejandra
authors.initials: MA
authors.lastname: Moya-León
authors.affiliation: Centro de Biología Molecular Vegetal, Departamento de Biología, Facultad de Ciencias, Universidad de Chile Santiago, Chile.
authors.forename: Lorena
authors.initials: L
authors.lastname: Norambuena
authors.affiliation: Centro de Biología Molecular Vegetal, Departamento de Biología, Facultad de Ciencias, Universidad de Chile Santiago, Chile.
authors.forename: Michael
authors.initials: M
authors.lastname: Handford
keywords: Fragaria chiloensis; LEA domain protein; abscisic acid; brassinosteroids; fruit development; non-climacteric