title: High-efficiency l-lactic acid production by Rhizopus oryzae using a novel modified one-step fermentation strategy.
abstract: In this study, lactic acid fermentation by Rhizopus oryzae was investigated using the two different fermentation strategies of one-step fermentation (OSF) and conventional fermentation (CF). Compared to CF, OSF reduced the demurrage of the production process and increased the production of lactic acid. However, the qp was significantly lower than during CF. Based on analysis of μ, qs and qp, a novel modified OSF strategy was proposed. This strategy aimed to achieve a high final concentration of lactic acid, and a high qp by R. oryzae. In this strategy, the maximum lactic acid concentration and productivity of the lactic acid production stage reached 158g/l and 5.45g/(lh), which were 177% and 366% higher, respectively, than the best results from CF. Importantly, the qp and yield did not decrease. This strategy is a convenient and economical method for l-lactic acid fermentation by R. oryzae.
authors.affiliation: Institute of Biomass Resources, Taizhou University, Jiaojiang 318000, Zhejiang, PR China. Electronic address: fuyq@tzc.edu.cn.
authors.forename: Yong-Qian
authors.initials: YQ
authors.lastname: Fu
authors.affiliation: Institute of Biomass Resources, Taizhou University, Jiaojiang 318000, Zhejiang, PR China.
authors.forename: Long-Fei
authors.initials: LF
authors.lastname: Yin
authors.affiliation: Institute of Biomass Resources, Taizhou University, Jiaojiang 318000, Zhejiang, PR China.
authors.forename: Hua-Yue
authors.initials: HY
authors.lastname: Zhu
authors.affiliation: Institute of Biomass Resources, Taizhou University, Jiaojiang 318000, Zhejiang, PR China.
authors.forename: Ru
authors.initials: R
authors.lastname: Jiang
mesh_terms: D019149:Bioreactors; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D005285:Fermentation; D007218:Industrial Microbiology; D007700:Kinetics; D019344:Lactic Acid / Q000096:biosynthesis*; D009584:Nitrogen / Q000737:chemistry; D010461:Peptones / Q000737:chemistry; D012233:Rhizopus / Q000378:metabolism*; D013696:Temperature
chemical_list: D003470:Culture Media; D010461:Peptones; D019344:Lactic Acid; D009584:Nitrogen
keywords: Fed-batch; High-efficiency production; One-step fermentation strategy; Rhizopus oryzae; l-Lactic acid