title: Human Papilloma Virus Persistence after Cone Excision in Women with Cervical High Grade Squamous Intraepithelial Lesion: A Prospective Study.
abstract: Background. Persistent human papillomavirus (HPV) infection is a necessary event in cervical cancer tumorigenesis. Our objectives were to estimate the rate of HPV infection persistence after large loop excision of the transformation zone (LEEP) in patients with high grade squamous intraepithelial lesions (HSIL) and to investigate if HPV persistence is type related. Methods. We conducted a prospective study on 89 patients with HSIL treated with LEEP. DNA HPV was performed before surgery and at 6, 12, and 18 months after LEEP. Results. Four patients were excluded from the study. The HPV persistence in the remaining 85 patients was 32.95% (6 months), 14.12% (12 months), and 10.59% (18 months). Type 16 had the highest persistence rate, 23.5% (6 months), 11.8% (12 months), and 8.2% (18 months). Coinfection was found to be 54.12% before LEEP and 18.8% (6 months), 4.7% (12 months), and 3.5% (18 months) after LEEP. The rate of coinfections including type 16 was 46.83% of all coinfections. Coinfection including type 16 was not correlated with higher persistence rate compared to infection with type 16 only. Conclusions. HPV infection is not completely eradicated by LEEP in patients with HSIL lesion on PAP smear. HPV persistence after LEEP is influenced by HPV type. HPV type 16 has the highest persistence rate.
authors.affiliation: Department of Obstetrics and Gynecology, University of Medicine and Pharmacy "Victor Babeş", Timişoara, 300041 Timiș, Romania.
authors.forename: Laurențiu
authors.initials: L
authors.lastname: Pirtea
authors.affiliation: Department of Obstetrics and Gynecology, University of Medicine and Pharmacy "Victor Babeş", Timişoara, 300041 Timiș, Romania.
authors.forename: Dorin
authors.initials: D
authors.lastname: Grigoraş
authors.affiliation: Department of Anatomy, University of Medicine and Pharmacy "Victor Babeş", Timişoara, 300041 Timiș, Romania.
authors.forename: Petru
authors.initials: P
authors.lastname: Matusz
authors.affiliation: Department of Obstetrics and Gynecology, County Hospital Timişoara, 300202 Timiș, Romania.
authors.forename: Marilena
authors.initials: M
authors.lastname: Pirtea
authors.affiliation: Department of Informatics and Statistics, University of Medicine and Pharmacy "Victor Babeş", Timişoara, 300041 Timiș, Romania.
authors.forename: Lavinia
authors.initials: L
authors.lastname: Moleriu
authors.affiliation: Department of Informatics and Statistics, University of Medicine and Pharmacy "Victor Babeş", Timişoara, 300041 Timiș, Romania.
authors.forename: Anca
authors.initials: A
authors.lastname: Tudor
authors.affiliation: Department of Surgery, University of Medicine and Pharmacy "Victor Babeş", Timişoara, 300041 Timiș, Romania.
authors.forename: Răzvan
authors.initials: R
authors.lastname: Ilina
authors.affiliation: Department of Obstetrics and Gynecology, County Hospital Timişoara, 300202 Timiș, Romania.
authors.forename: Cristina
authors.initials: C
authors.lastname: Secoşan
authors.affiliation: Department of Microbiology, University of Medicine and Pharmacy "Victor Babeş", Timişoara, Piata Eftimie Murgu, No. 1, 300041 Timiș, Romania.
authors.forename: Florin
authors.identifier: 0000-0001-6133-0204
authors.initials: F
authors.lastname: Horhat
authors.affiliation: Department of Surgery, University of Medicine and Pharmacy "Victor Babeş", Timişoara, 300041 Timiș, Romania.
authors.forename: Octavian
authors.initials: O
authors.lastname: Mazilu