title: Long non-coding RNA SPRY4-IT1 pormotes colorectal cancer metastasis by regulate epithelial-mesenchymal transition.
abstract: Colorectal cancer (CRC) remains one of the most common cancers worldwide. Increasing evidence indicates that SPRY4 intronic transcript 1 (SPRY4-IT1) regulate cell growth, differentiation, apoptosis, and cancer progression. However, the expression and function of SPRY4-IT1 in the progression of CRC remains largely unknown. Here, we reported that SPRY4-IT1 was upregulated in CRC. Increased SPRY4-IT1 expression in CRC was associated with larger tumor size and higher clinical stage. In vitro experiments revealed that SPRY4-IT1 knockdown significantly inhibited CRC cell proliferation by causing G1 arrest and promoting apoptosis, whereas SPRY4-IT1 overexpression promoted cell proliferation. Further functional assays indicated that SPRY4-IT1 overexpression significantly promoted cell migration and invasion by regulate the epithelial-mesenchymal transition (EMT). Taken together, our study demonstrates that SPRY4-IT1 could act as a functional oncogene in CRC, as well as a potential therapeutic target to inhibit CRC metastasis.
authors.affiliation: Department of General Surgery, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, P.R. China.
authors.forename: Fei
authors.initials: F
authors.lastname: Shen
authors.affiliation: Department of General Surgery, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, P.R. China.
authors.forename: Wen-Song
authors.initials: WS
authors.lastname: Cai
authors.affiliation: Department of General Surgery, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, P.R. China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Feng
authors.affiliation: Department of General Surgery, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, P.R. China.
authors.forename: Ji-Wei
authors.initials: JW
authors.lastname: Chen
authors.affiliation: Department of General Surgery, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, P.R. China.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Feng
authors.affiliation: Experimental Medical Research Center, Guangzhou Medical University, Guangzhou, P.R. China.
authors.forename: Qi-Cai
authors.initials: QC
authors.lastname: Liu
authors.affiliation: Department of General Surgery, Huizhou First People's Hospital, Huizhou, P.R. China.
authors.forename: Yong-Ping
authors.initials: YP
authors.lastname: Fang
authors.affiliation: Department of General Surgery, Huizhou First People's Hospital, Huizhou, P.R. China.
authors.forename: Kun-Ping
authors.initials: KP
authors.lastname: Li
authors.affiliation: Department of General Surgery, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, P.R. China.
authors.forename: Huan-Qing
authors.initials: HQ
authors.lastname: Xiao
authors.affiliation: Department of General Surgery, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Cao
authors.affiliation: Department of General Surgery, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, P.R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Xu
mesh_terms: D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000235:genetics*; D015153:Blotting, Western; D002465:Cell Movement; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000235:genetics / Q000556:secondary*; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D062085:RNA, Long Noncoding / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor; D062085:RNA, Long Noncoding; D012333:RNA, Messenger; C000605823:long noncoding RNA SPRY4-IT1, human
keywords: CRC; EMT; LncRNA; SPRY4-IT1; metastasis