title: Data set of optimal parameters for colorimetric red assay of epoxide hydrolase activity.
abstract: The data presented in this article are related to the research article entitled "Epoxide hydrolase of Trichoderma reesei: Biochemical properties and conformational characterization" [1]. Epoxide hydrolases (EHs) are enzymes that catalyze the hydrolysis of epoxides to the corresponding vicinal diols. This article describes the optimal parameters for the colorimetric red assay to determine the enzymatic activity, with an emphasis on the characterization of the kinetic parameters, pH optimum and thermal stability of this enzyme. The effects of reagents that are not resistant to oxidation by sodium periodate on the reactions can generate false positives and interfere with the final results of the red assay.
authors.affiliation: Escola de Artes, Ciências e Humanidades, Universidade de São Paulo, Av. Arlindo Béttio, 1000, São Paulo, Brazil.
authors.forename: Gabriel Stephani
authors.initials: GS
authors.lastname: de Oliveira
authors.affiliation: Escola de Artes, Ciências e Humanidades, Universidade de São Paulo, Av. Arlindo Béttio, 1000, São Paulo, Brazil.
authors.forename: Patricia Pereira
authors.initials: PP
authors.lastname: Adriani
authors.affiliation: Escola de Artes, Ciências e Humanidades, Universidade de São Paulo, Av. Arlindo Béttio, 1000, São Paulo, Brazil.
authors.forename: Flavia Garcia
authors.initials: FG
authors.lastname: Borges
authors.affiliation: Instituto Butantan, Av. Vital Brasil, 1500, São Paulo, Brazil.
authors.forename: Adriana Rios
authors.initials: AR
authors.lastname: Lopes
authors.affiliation: Escola de Artes, Ciências e Humanidades, Universidade de São Paulo, Av. Arlindo Béttio, 1000, São Paulo, Brazil.
authors.forename: Patricia T
authors.initials: PT
authors.lastname: Campana
authors.affiliation: Escola de Artes, Ciências e Humanidades, Universidade de São Paulo, Av. Arlindo Béttio, 1000, São Paulo, Brazil.
authors.forename: Felipe S
authors.initials: FS
authors.lastname: Chambergo
keywords: Colorimetric red assay; Epoxide hydrolase; Kinetic parameters