title: [Role of NO signal in ABA-induced phenolic acids accumulation in Salvia miltiorrhiza hairy roots].
abstract: To investigate roles of nitric oxide (NO) signal in accumulations of phenolic acids in abscisic.acid (ABA)-induced Salvia miltiorrhiza hairy roots, S. miltiorrhiza hairy roots were treated with different concentrations of sodium nitroprusside (SNP)-an exogenous NO donor, for 6 days, and contents of phenolic acids in the hairy roots are determined. Then with treatment of ABA and NO scavenger (2-(4-carboxy-2-phenyl)-4,4,5,5-tetramethylimidazoline-1- oxyl-3-oxide, c-PTIO) or NO synthase inhibitor (NG-nitro-L-arginine methyl ester, L-NAME), contents of phenolic acids and expression levels of three key genes involved in phenolic acids biosynthesis were detected. Phenolic acids production in S. miltiorrhiza hairy roots was most significantly improved by 100 µmoL/L SNP. Contents of RA and salvianolic acid B increased by 3 and 4 folds. ABA significantly improved transcript levels of PAL (phenylalanine ammonia lyase), TAT (tyrosine aminotransferase) and RAS (rosmarinic acid synthase), and increased phenolic acids accumulations. However, with treatments of ABA+c-PTIO or ABA+L-NAME, accumulations of phenolic acids and expression levels of the three key genes were significantly inhibited. Both NO and ABA can increase accumulations of phenolic acids in S. miltiorrhiza hairy roots. NO signal probably mediates the ABA-induced phenolic acids production.
authors.forename: Lihong
authors.initials: L
authors.lastname: Shen
authors.forename: Jiahui
authors.initials: J
authors.lastname: Ren
authors.forename: Wenfang
authors.initials: W
authors.lastname: Jin
authors.forename: Ruijie
authors.initials: R
authors.lastname: Wang
authors.forename: Chunhong
authors.initials: C
authors.lastname: Ni
authors.forename: Mengjiao
authors.initials: M
authors.lastname: Tong
authors.forename: Zongsuo
authors.initials: Z
authors.lastname: Liang
authors.forename: Dongfeng
authors.initials: D
authors.lastname: Yang
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology*; D001572:Benzofurans / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology; D062385:Hydroxybenzoates / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D010650:Phenylalanine Ammonia-Lyase / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism*; D027561:Salvia miltiorrhiza / Q000378:metabolism*; D014444:Tyrosine Transaminase / Q000378:metabolism; D053630:Depsides
chemical_list: D000040:Abscisic Acid; D001572:Benzofurans; D016166:Free Radical Scavengers; D062385:Hydroxybenzoates; D009569:Nitric Oxide; D010650:Phenylalanine Ammonia-Lyase; D014444:Tyrosine Transaminase; C076944:salvianolic acid B; D053630:Depsides; C017616:phenolic acid