title: Relationship between the Balance of Hypertrophic/Hyperplastic Adipose Tissue Expansion and the Metabolic Profile in a High Glucocorticoids Model.
abstract: Adipose tissue (AT) expansion is the result of two processes: hyperplasia and hypertrophy; and both, directly or indirectly, depend on the adipogenic potential of adipocyte precursor cells (APCs). Glucocorticoids (GCs) have a potent stimulatory effect on terminal adipogenesis; while their effects on early stages of adipogenesis are largely unknown. In the present work, we study, in a model of high GC levels, the adipogenic potential of APCs from retroperitoneal AT (RPAT) and its relationship with RPAT mass expansion. We employed a model of hyper-adiposity (30- and 60-day-old rats) due to high endogenous GC levels induced by neonatal treatment with l-monosodium glutamate (MSG). We found that the RPAT APCs from 30-day-old MSG rats showed an increased adipogenic capacity, depending on the APCs' competency, but not in their number. Analyses of RPAT adipocyte diameter revealed an increase in cell size, regardless of the rat age, indicating the prevalence of a hypertrophic process. Moreover, functional RPAT alterations worsened in 60-day-old rats, suggesting that the hyperplastic AT expansion found in 30-day-old animals might have a protective role. We conclude that GCs chronic excess affects APCs' adipogenic capacity, modifying their competency. This change would modulate the hyperplastic/hypertrophic balance determining healthy or unhealthy RPAT expansion and, therefore, its functionality.
authors.affiliation: Neuroendocrinology Laboratory, Multidisciplinary Institute of Cellular Biology (IMBICE, CICPBA-CONICET-UNLP), Calles 526 10 y 11, La Plata 1900, Argentina. gzubiria@imbice.gov.ar.|Biology Department, School of Exact Sciences, Universidad Nacional de La Plata, La Plata 1900, Argentina. gzubiria@imbice.gov.ar.
authors.forename: María Guillermina
authors.initials: MG
authors.lastname: Zubiría
authors.affiliation: Neuroendocrinology Laboratory, Multidisciplinary Institute of Cellular Biology (IMBICE, CICPBA-CONICET-UNLP), Calles 526 10 y 11, La Plata 1900, Argentina. aalzamendi@imbice.gov.ar.
authors.forename: Ana
authors.initials: A
authors.lastname: Alzamendi
authors.affiliation: Institute of Immunological and Physiopathological Research (IIFP, CONICET-UNLP), School of Exact Sciences, Universidad Nacional de La Plata, La Plata 1900, Argentina. gmoreno@iifp.laplata-conicet.gov.ar.
authors.forename: Griselda
authors.initials: G
authors.lastname: Moreno
authors.affiliation: Neuroendocrinology Laboratory, Multidisciplinary Institute of Cellular Biology (IMBICE, CICPBA-CONICET-UNLP), Calles 526 10 y 11, La Plata 1900, Argentina. andreaeportales@gmail.com.|Biology Department, School of Exact Sciences, Universidad Nacional de La Plata, La Plata 1900, Argentina. andreaeportales@gmail.com.
authors.forename: Andrea
authors.initials: A
authors.lastname: Portales
authors.affiliation: Neuroendocrinology Laboratory, Multidisciplinary Institute of Cellular Biology (IMBICE, CICPBA-CONICET-UNLP), Calles 526 10 y 11, La Plata 1900, Argentina. dcastrogiovanni@yahoo.com.
authors.forename: Daniel
authors.initials: D
authors.lastname: Castrogiovanni
authors.affiliation: Center of Experimental and Applied Endocrinology (CENEXA, UNLP-CONICET, PAHO/WHO Collaborating Center for Diabetes), La Plata Medical School, Universidad Nacional de La Plata, La Plata 1900, Argentina. spinedi@cenexa.org.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Spinedi
authors.affiliation: Neuroendocrinology Laboratory, Multidisciplinary Institute of Cellular Biology (IMBICE, CICPBA-CONICET-UNLP), Calles 526 10 y 11, La Plata 1900, Argentina. agiovamba@imbice.gov.ar.|Biology Department, School of Exact Sciences, Universidad Nacional de La Plata, La Plata 1900, Argentina. agiovamba@imbice.gov.ar.
authors.forename: Andrés
authors.initials: A
authors.lastname: Giovambattista
mesh_terms: D017667:Adipocytes / Q000378:metabolism; D050156:Adipogenesis / Q000502:physiology; D050154:Adiposity / Q000502:physiology; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D002478:Cells, Cultured; D003345:Corticosterone / Q000097:blood; D004195:Disease Models, Animal; D005938:Glucocorticoids / Q000097:blood*; D006965:Hyperplasia / Q000097:blood / Q000150:complications; D006984:Hypertrophy / Q000097:blood / Q000150:complications; D007328:Insulin / Q000097:blood; D050152:Intra-Abdominal Fat / Q000378:metabolism*; D020738:Leptin / Q000097:blood; D008297:Male; D008314:Malonates / Q000009:adverse effects; D009765:Obesity / Q000097:blood*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D005938:Glucocorticoids; D007328:Insulin; D020738:Leptin; D008314:Malonates; C030290:malonic acid; D003345:Corticosterone
keywords: adipogenesis; adipogenic competency; cell determination; retroperitoneal adipose tissue; stromal vascular fraction cells