title: Pleiotropic effects of survivin in vascular endothelial cells.
abstract: To assess the effects of survivin (SVV)in vascular endothelial cells.
  
  
  
  In this study, we applied a gain-of-function approach and ectopically expressed SVV in rat aortic endothelial cells (RAECs) using a SVV-expressing adenovirus. The resulting SVV expression on the steady-state mRNA and protein level in RAECs was determined by reverse transcription quantitative PCR and Western blot, respectively. Cell viability, apoptosis, and migration were assessed in vitro by CCK-8 assay, flow cytometry, and transwell assay, respectively. The effect of SVV on in vivo angiogenesis was evaluated by immunohistochemistry in nude mice. Non-infected RAECs and those infected with GFP-expressing control adenovirus were used as controls.
  
  
  
  Compared to non-infected or control adenovirus-infected RAECs in vitro, SVV-expressing cells had increased viability and migratory capability, but reduced apoptosis. In vivo, SVV-expressing RAECs were associated with a higher level of angiogenesis.
  
  
  
  SVV is a positive regulator of endothelial cell survival and migration, and thus, stabilizes endothelial cells and stimulates angiogenesis.
authors.affiliation: Department of Vascular Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China 400016.
authors.forename: Haolong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Vascular Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China 400016. Electronic address: zzhhll0517@gmail.com.
authors.forename: Xuehu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Head and Neck Surgery, Chongqing Cancer Institute, China 400016.
authors.forename: Chengyao
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Vascular Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China 400016.
authors.forename: Fangyu
authors.initials: F
authors.lastname: Zhu
authors.affiliation: Department of Vascular Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China 400016.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Department of Gastrointestinal Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China 400016.
authors.forename: Xudong
authors.initials: X
authors.lastname: Peng
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D017209:Apoptosis; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000378:metabolism* / Q000637:transplantation; D005260:Female; D005822:Genetic Vectors; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D018919:Neovascularization, Physiologic; D051381:Rats; D015398:Signal Transduction; D000077022:Survivin; D013997:Time Factors; D014162:Transfection
chemical_list: C516989:Birc5 protein, rat; D008869:Microtubule-Associated Proteins; D000077022:Survivin
keywords: aurora B; periphery artery disease; survivin; vascular endothelial cells