title: Association of genetic variants in the 3'UTR of HLA-G with Recurrent Pregnancy Loss.
abstract: Human Leukocyte Antigen (HLA)-G is involved in reprogramming immune responses at fetal-maternal interface during pregnancy. We evaluated the genetic diversity of the 3' Un-Translated Region (UTR) of HLA-G, previously associated with HLA-G mRNA post-transcriptional regulation, in women with unexplained Recurrent Pregnancy Loss (RPL), with 2 pregnancy losses (RPL-2, n=28), or 3 or more pregnancy losses (RPL-3, n=24), and in 30 women with a history of successful pregnancy. Results showed in RPL-2, but not in RPL-3, women compared to controls: i) higher frequency of the 14bp Ins allele, in single and in double copy; ii) significantly lower frequency of DelG/X genotype, iii) reduced frequency of the UTR-2, and UTR-3 haplotypes; iv) higher frequencies of the UTR-5, UTR-7, and UTR-8 haplotypes. This pilot study supports the relevance of performing 3'UTR HLA-G genetic screening, not limited to a specific polymorphism, but considering the extended haplotypes, as a possible predictor of pregnancy outcome.
authors.affiliation: San Raffaele Telethon Institute for Gene Therapy (SR-Tiget), Division of Regenerative Medicine, Stem Cells and Gene Therapy, IRCCS San Raffaele Scientific Institute, Via Olgettina 60, 20132 Milan, Italy.
authors.forename: Giada
authors.initials: G
authors.lastname: Amodio
authors.affiliation: Division of Immunology, Transplantation and Infectious Disease, IRCCS San Raffaele Scientific Institute, Via Olgettina 58, 20132 Milan, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Canti
authors.affiliation: Obstetric and Gynecology Unit, IRCCS San Raffaele Scientific Institute, Milan, Italy.
authors.forename: Luana
authors.initials: L
authors.lastname: Maggio
authors.affiliation: Obstetric and Gynecology Unit, IRCCS San Raffaele Scientific Institute, Milan, Italy.
authors.forename: Susanna
authors.initials: S
authors.lastname: Rosa
authors.affiliation: Obstetric and Gynecology Unit, IRCCS San Raffaele Scientific Institute, Milan, Italy.
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Castiglioni
authors.affiliation: Division of Immunology, Transplantation and Infectious Disease, IRCCS San Raffaele Scientific Institute, Via Olgettina 58, 20132 Milan, Italy; Vita-Salute San Raffaele University, Via Olgettina 60, 20132 Milan, Italy.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Rovere-Querini
authors.affiliation: San Raffaele Telethon Institute for Gene Therapy (SR-Tiget), Division of Regenerative Medicine, Stem Cells and Gene Therapy, IRCCS San Raffaele Scientific Institute, Via Olgettina 60, 20132 Milan, Italy. Electronic address: gregori.silvia@hsr.it.
authors.forename: Silvia
authors.initials: S
authors.lastname: Gregori
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics*; D000026:Abortion, Habitual / Q000175:diagnosis / Q000235:genetics*; D000328:Adult; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005820:Genetic Testing; D005838:Genotype; D059951:HLA-G Antigens / Q000235:genetics*; D006801:Humans; D007558:Italy; D010865:Pilot Projects; D011110:Polymorphism, Genetic; D011247:Pregnancy; D000090822:Ribosomal Protein L3
chemical_list: D020413:3' Untranslated Regions; D059951:HLA-G Antigens; C000716547:RPL3 protein, human; D000090822:Ribosomal Protein L3
keywords: 3′UTR polymorphisms of HLA-G; HLA-G; Unexplained Recurrent Pregnancy Loss (RPL)