title: Supplementation with Major Royal-Jelly Proteins Increases Lifespan, Feeding, and Fecundity in Drosophila.
abstract: The major royal-jelly proteins (MRJPs) are the main constituents responsible for the specific physiological role of royal jelly (RJ) in honeybees. Male and female Drosophila flies were fed diets containing either no MRJPs (A) or casein (B) at 1.25% (w/w) of diet or MRJPs at 1.25% (C), 2.50% (D), or 5.00% (E). Diets B, C, D, and E increased mean lifespan by 4.3%, 9.0%, 12.4%, and 13.9% in males and by 5.8%, 9.7%, 20.0%, and 11.8% in females in comparison to results from diet A, respectively. The diet supplemented with 2.50% MRJPs seems to have the optimal dose to improve both physiological and biochemical measures related to aging in both sexes. Interestingly, lifespan extension by MRJPs in Drosophila was positively associated with feeding and fecundity and up-regulation of copper and zinc-superoxide dismutase (CuZn-SOD) and the Egfr-mediated signaling pathway. This study provides strong evidence that MRJPs are important components of RJ for prolonging lifespan in Drosophila.
authors.affiliation: Department of Food Science and Nutrition, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University , Hangzhou, 310058, China.
authors.forename: Xiao-Xuan
authors.initials: XX
authors.lastname: Xin
authors.affiliation: Department of Food Science and Nutrition, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University , Hangzhou, 310058, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Food Science and Nutrition, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University , Hangzhou, 310058, China.
authors.forename: Di
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Food Science and Nutrition, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University , Hangzhou, 310058, China.
authors.forename: Fa
authors.initials: F
authors.lastname: Xiao
authors.affiliation: USDA ARS Nutritional Genomics Laboratory, JM-USDA Human Nutrition Research Center on Aging at Tufts University , Boston, Massachusetts 02111, United States.
authors.forename: Laurence D
authors.initials: LD
authors.lastname: Parnell
authors.affiliation: Department of Statistics, The University of Georgia , Athens, Georgia 30602, United States.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Statistics, The University of Georgia , Athens, Georgia 30602, United States.
authors.forename: Liang
authors.initials: L
authors.lastname: Liu
authors.affiliation: USDA ARS Nutritional Genomics Laboratory, JM-USDA Human Nutrition Research Center on Aging at Tufts University , Boston, Massachusetts 02111, United States.
authors.forename: Jose M
authors.initials: JM
authors.lastname: Ordovas
authors.affiliation: USDA ARS Nutritional Genomics Laboratory, JM-USDA Human Nutrition Research Center on Aging at Tufts University , Boston, Massachusetts 02111, United States.
authors.forename: Chao-Qiang
authors.initials: CQ
authors.lastname: Lai
authors.affiliation: Department of Food Science and Nutrition, Fuli Institute of Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University , Hangzhou, 310058, China.
authors.forename: Li-Rong
authors.initials: LR
authors.lastname: Shen
mesh_terms: D000818:Animals; D001516:Bees / Q000378:metabolism; D019587:Dietary Supplements / Q000032:analysis; D004330:Drosophila / Q000254:growth & development / Q000502:physiology*; D005227:Fatty Acids / Q000378:metabolism*; D005247:Feeding Behavior; D005260:Female; D005298:Fertility; D019476:Insect Proteins / Q000378:metabolism*; D008136:Longevity; D008297:Male; D000099219:Royal Jelly
chemical_list: D005227:Fatty Acids; D019476:Insect Proteins; D000099219:Royal Jelly
keywords: Drosophila; Egfr-mediated signaling pathway; antioxidation activity; fecundity and feeding; lifespan; major royal jelly proteins