title: The complete genome of the tospovirus Zucchini lethal chlorosis virus.
abstract: Zucchini lethal chlorosis virus (ZLCV) causes significant losses in the production of cucurbits in Brazil. This virus belongs to the genus Tospovirus (family Bunyaviridae) and seems to be exclusively transmitted by Frankliniella zucchini (Thysanoptera). Tospoviruses have a tripartite and single-stranded RNA genome classified as S (Small), M (Medium) and L (Large) RNAS. Although ZLCV was identified as a member of the genus Tospovirus in 1999, its complete genome had not been sequenced until now.
  
  
  
  We sequenced the full-length genome of two ZLCV isolates named ZLCV-SP and ZLCV-DF. The phylogenetic analysis showed that ZLCV-SP and ZLCV-DF clustered with the previously reported isolate ZLCV-BR09. Their proteins were closely related, except the non-structural protein (NSm), which was highly divergent (approximately 90 % identity). All viral proteins clustered similarly in our phylogenetic analysis, excluding that these ZLCV isolates have originated from reassortment events of different tospovirus species.
  
  
  
  Here we report for the first time the complete genome of two ZLCV isolates that were found in the field infecting zucchini and cucumber.
authors.affiliation: Laboratory of Virology, Department of Cell Biology, University of Brasilia, Brasilia, DF, 70910-900, Brazil.
authors.forename: R N
authors.initials: RN
authors.lastname: Lima
authors.affiliation: Laboratory of Virology, Department of Cell Biology, University of Brasilia, Brasilia, DF, 70910-900, Brazil.
authors.forename: A S
authors.initials: AS
authors.lastname: De Oliveira
authors.affiliation: Laboratory of Virology, Department of Cell Biology, University of Brasilia, Brasilia, DF, 70910-900, Brazil.
authors.forename: M O
authors.initials: MO
authors.lastname: Leastro
authors.affiliation: Laboratory of Virology, Department of Cell Biology, University of Brasilia, Brasilia, DF, 70910-900, Brazil.
authors.forename: R
authors.initials: R
authors.lastname: Blawid
authors.affiliation: Laboratory of Virology, Department of Cell Biology, University of Brasilia, Brasilia, DF, 70910-900, Brazil.
authors.forename: T
authors.initials: T
authors.lastname: Nagata
authors.affiliation: Laboratory of Virology, Department of Cell Biology, University of Brasilia, Brasilia, DF, 70910-900, Brazil.
authors.forename: R O
authors.initials: RO
authors.lastname: Resende
authors.affiliation: Laboratory of Virology, Department of Cell Biology, University of Brasilia, Brasilia, DF, 70910-900, Brazil. flmelo@unb.br.
authors.forename: F L
authors.initials: FL
authors.lastname: Melo
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D028464:Cucurbita / Q000821:virology*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D016415:Sequence Alignment; D017865:Tospovirus / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D014764:Viral Proteins
keywords: Genome; Illumina; Tospovirus; ZLCV; Zucchini