title: Comparative Evaluation of the Antimicrobial Efficacy of Three Immersion Chemical Disinfectants on Clinically Derived Poly(Vinyl Siloxane) Impressions.
abstract: OBJECTIVE
  To comparatively evaluate the antimicrobial efficacy of freshly prepared electrolyzed oxidizing water (EOW) with that of 2.4% glutaraldehyde (GA) and 1% sodium hypochlorite (SH) on clinically derived poly vinyl siloxane (PVS) impressions.
  
  
  METHODS
  Four clinically derived PVS impressions each were obtained from 10 subjects. One impression from each subject was randomly kept as control (group I), without disinfection. The remaining three impressions were randomly disinfected by immersion in either 2.4% GA (group II), 1% SH (group III), or freshly prepared EOW (group IV). The samples were subjected to microbial culture individually on brain heart infusion (BHI) agar medium. The organisms isolated were confirmed by visual examination and gram staining. Mean colony forming units (CFU) were counted, log10reduction obtained and compared. Data were statistically analyzed using one-way ANOVA, followed by multiple comparisons using Tukey-HSD post hoc tests (p < 0.05).
  
  
  RESULTS
  Streptococci, Staphylococci, Pseudomonas, Candida, Proteus, Klebsiella, and E. coli were isolated from all impressions including the controls, except those disinfected by EOW. All three disinfectants showed significant reduction in CFU and log10reduction values as compared to the controls. EOW showed significantly higher reduction in log10values compared to GA and SH, whereas GA and SH showed similar reductions. EOW, GA, and SH showed kill rates of 100%, 99.60%, and 99.82%, respectively.
  
  
  CONCLUSIONS
  EOW showed significant antimicrobial efficacy as evidenced by reduction in log10values when used as an immersion disinfectant for PVS impressions when compared with the results obtained using GA and SH. These results need further investigation.
  
  
  CONCLUSIONS
  EOW shows high antimicrobial efficacy when used as an immersion disinfectant as compared to GA and SH for clinically derived PVS impressions.
authors.affiliation: Department of Prosthodontics, Ragas Dental College and Hospital, Chennai, India.
authors.forename: Vidhya
authors.initials: V
authors.lastname: Jeyapalan
authors.affiliation: Department of Prosthodontics, Ragas Dental College and Hospital, Chennai, India.
authors.forename: Chitra Shankar
authors.initials: CS
authors.lastname: Krishnan
authors.affiliation: Department of Prosthodontics, Ragas Dental College and Hospital, Chennai, India.
authors.forename: Hariharan
authors.initials: H
authors.lastname: Ramasubramanian
authors.affiliation: Department of Prosthodontics, Ragas Dental College and Hospital, Chennai, India.
authors.forename: Jayakrishnakumar
authors.initials: J
authors.lastname: Sampathkumar
authors.affiliation: Department of Prosthodontics, Ragas Dental College and Hospital, Chennai, India.
authors.forename: N S
authors.initials: NS
authors.lastname: Azhagarasan
authors.affiliation: Department of Prosthodontics, Ragas Dental College and Hospital, Chennai, India.
authors.forename: Madhusudan
authors.initials: M
authors.lastname: Krishnan
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D000890:Anti-Infective Agents / Q000737:chemistry*; D015169:Colony Count, Microbial; D019551:Dental Disinfectants / Q000737:chemistry*; D003760:Dental Impression Materials / Q000737:chemistry*; D003761:Dental Impression Technique; D004203:Disinfection / Q000379:methods*; D004866:Equipment Contamination / Q000517:prevention & control*; D005260:Female; D005976:Glutaral / Q000737:chemistry*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011145:Polyvinyls / Q000737:chemistry*; D012833:Siloxanes / Q000737:chemistry*; D012973:Sodium Hypochlorite / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D019551:Dental Disinfectants; D003760:Dental Impression Materials; D011145:Polyvinyls; D012833:Siloxanes; C034183:vinyl polysiloxane; D012973:Sodium Hypochlorite; D005976:Glutaral
keywords: Electrolyzed oxidizing water; addition silicone; antimicrobial effect; impression disinfection; microbial reduction