title: Effect of Dietary-Resistant Starch on Inhibition of Colonic Preneoplasia and Wnt Signaling in Azoxymethane-Induced Rodent Models.
abstract: Dietary fiber has been reported to prevent preneoplastic colon lesions. The aim of this study was to determine the effect of resistant starches, novel dietary fibers, on the development of colonic preneoplasia and Wnt signaling in azoxymethane (AOM)-treated rats and mice fed resistant starches at 55% of the diet after AOM treatment. Another objective was to determine the effect of resistant starches on the development of preneoplasia in rats treated with antibiotics (Ab), administered between AOM treatment and resistant starch feeding. Diets containing resistant starches, high-amylose (HA7), high-amylose-octenyl succinic anhydride (OS-HA7), or high-amylose-stearic acid (SA-HA7) were compared with control cornstarch (CS). The resistant starch content of the diets did not alter the yield of colonic lesions but animals treated with AOM and fed the diet with the highest resistant starch content, SA-HA7 developed the highest average aberrant crypt foci (ACF) per animal. Mice fed the OS-HA7 diet had decreased expression of some upstream Wnt genes in the colonic crypts. This study suggests that further research is needed to determine if resistant starch impacts colon carcinogenesis in rodents.
authors.affiliation: a Department of Food Science and Human Nutrition , Iowa State University , Ames , Iowa.
authors.forename: Bridget
authors.initials: B
authors.lastname: Nelson
authors.affiliation: b Department of Veterinary Pathology , Iowa State University , Ames , Iowa.
authors.forename: Nicole
authors.initials: N
authors.lastname: Cray
authors.affiliation: a Department of Food Science and Human Nutrition , Iowa State University , Ames , Iowa.
authors.forename: Yongfeng
authors.initials: Y
authors.lastname: Ai
authors.affiliation: c Department of Statistics , Iowa State University , Ames , Iowa.
authors.forename: Yinan
authors.initials: Y
authors.lastname: Fang
authors.affiliation: c Department of Statistics , Iowa State University , Ames , Iowa.
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.affiliation: b Department of Veterinary Pathology , Iowa State University , Ames , Iowa.|d Pathogenesis, LLC , Gainesville , Florida , USA.
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Whitley
authors.affiliation: a Department of Food Science and Human Nutrition , Iowa State University , Ames , Iowa.
authors.forename: Diane
authors.initials: D
authors.lastname: Birt
mesh_terms: D058739:Aberrant Crypt Foci / Q000378:metabolism / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control; D000818:Animals; D000900:Anti-Bacterial Agents / Q000009:adverse effects; D016588:Anticarcinogenic Agents / Q000378:metabolism / Q000627:therapeutic use*; D001397:Azoxymethane / Q000633:toxicity; D002273:Carcinogens / Q000633:toxicity; D003106:Colon / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology; D003110:Colonic Neoplasms / Q000378:metabolism / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control*; D000069196:Gastrointestinal Microbiome / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology; D008805:Mice, Inbred A; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D056692:Prebiotics; D011230:Precancerous Conditions / Q000378:metabolism / Q000382:microbiology / Q000473:pathology / Q000517:prevention & control*; D011916:Rats, Inbred F344; D000084922:Resistant Starch; D013213:Starch / Q000031:analogs & derivatives / Q000378:metabolism / Q000627:therapeutic use*; D013229:Stearic Acids / Q000378:metabolism / Q000627:therapeutic use; D013387:Succinic Anhydrides / Q000378:metabolism / Q000627:therapeutic use; D047368:Tumor Burden / Q000187:drug effects; D060449:Wnt Signaling Pathway / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D016588:Anticarcinogenic Agents; D002273:Carcinogens; D009363:Neoplasm Proteins; D056692:Prebiotics; D000084922:Resistant Starch; D013229:Stearic Acids; D013387:Succinic Anhydrides; C572111:high-amylose maize type 2 resistant starch, maize; C031183:stearic acid; C031801:succinic anhydride; D013213:Starch; D001397:Azoxymethane