title: Differentiation of ring-substituted regioisomers of amphetamine and methamphetamine by supercritical fluid chromatography.
abstract: Chromatographic differentiation of the ring-substituted regioisomers of amphetamine (AMP) and methamphetamine (MA) was performed by supercritical fluid chromatography (SFC). The behaviour of the retention against the changes of column temperature and co-solvent proportion was studied. The obtained information facilitated the optimization of the each regioisomer. As a result, 2-, 3-, and 4-ring-substituted analogues of AMP and MA with methyl, methoxy, fluoro, chloro, and bromo groups were separated, generally within 6 min. In addition, we found that the separation pattern of the examined regioisomers was classified into two, which depended on the electron donating/withdrawing effect of the substituent. Our results indicate that SFC could be used in forensic drug analysis for fast, reliable identification of structurally similar drugs of abuse. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba, 277-0882, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Segawa
authors.affiliation: National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba, 277-0882, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: T Iwata
authors.affiliation: National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba, 277-0882, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Yamamuro
authors.affiliation: National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba, 277-0882, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Kuwayama
authors.affiliation: National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba, 277-0882, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Tsujikawa
authors.affiliation: National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba, 277-0882, Japan.
authors.forename: Tatsuyuki
authors.initials: T
authors.lastname: Kanamori
authors.affiliation: National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba, 277-0882, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Inoue
mesh_terms: D000661:Amphetamine / Q000737:chemistry* / Q000302:isolation & purification; D000697:Central Nervous System Stimulants / Q000737:chemistry* / Q000302:isolation & purification; D025924:Chromatography, Supercritical Fluid; D008694:Methamphetamine / Q000737:chemistry* / Q000302:isolation & purification; D012997:Solvents; D013237:Stereoisomerism
chemical_list: D000697:Central Nervous System Stimulants; D012997:Solvents; D008694:Methamphetamine; D000661:Amphetamine
keywords: amphetamine and methamphetamine; new psychoactive substances; regioisomer; supercritical fluid chromatography