title: Fibrin Association at Hybrid Biointerfaces Made of Clot-Binding Peptides and Polythiophene.
abstract: The properties as biointerfaces of electroactive conducting polymer-peptide biocomposites formed by poly(3,4-ethylenedioxythiophene) (PEDOT) and CREKA or CR(NMe)EKA peptide sequences (where Glu has been replaced by N-methyl-Glu in the latter) have been compared. CREKA is a linear pentapeptide that recognizes clotted plasma proteins and selectively homes to tumors, while CR(NMe)EKA is an engineer to improve such properties by altering peptide-fibrin interactions. Differences between PEDOT-CREKA and PEDOT-CR(NMe)EKA reflect dissemblance in the organization of the peptides into the polymeric matrix. Both peptides affect fibrinogen thrombin-catalyzed polymerization causing the immediate formation of fibrin, whereas in the absence of thrombin this phenomenon is only observed for CR(NMe)EKA. Consistently, the fibrin-adsorption capacity is higher for PEDOT-CR(NMe)EKA than for PEDOT-CREKA, even though in both cases adsorbed fibrin exhibits round-like morphologies rather than the characteristic fibrous structure. PEDOT-peptide films coated with fibrin are selective in terms of cell adhesion, promoting the attachment of metastatic cells with respect to normal cells.
authors.affiliation: Departament d'Enginyeria Química, E. T. S. d'Enginyeria Industrial de Barcelona, Universitat Politècnica de Catalunya, Diagonal 647, Barcelona, E-08028, Spain.|Centre for Research in Nano-Engineering, Universitat Politècnica de Catalunya, Edifici C', C/Pasqual i Vila s/n, Barcelona, E-08028, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Puiggalí-Jou
authors.affiliation: Departament d'Enginyeria Química, E. T. S. d'Enginyeria Industrial de Barcelona, Universitat Politècnica de Catalunya, Diagonal 647, Barcelona, E-08028, Spain. luis.javier.del.valle@upc.edu.|Centre for Research in Nano-Engineering, Universitat Politècnica de Catalunya, Edifici C', C/Pasqual i Vila s/n, Barcelona, E-08028, Spain. luis.javier.del.valle@upc.edu.
authors.forename: Luis J
authors.initials: LJ
authors.lastname: Del Valle
authors.affiliation: Departament d'Enginyeria Química, E. T. S. d'Enginyeria Industrial de Barcelona, Universitat Politècnica de Catalunya, Diagonal 647, Barcelona, E-08028, Spain.|Centre for Research in Nano-Engineering, Universitat Politècnica de Catalunya, Edifici C', C/Pasqual i Vila s/n, Barcelona, E-08028, Spain.
authors.forename: Elaine
authors.initials: E
authors.lastname: Armelin
authors.affiliation: Departament d'Enginyeria Química, E. T. S. d'Enginyeria Industrial de Barcelona, Universitat Politècnica de Catalunya, Diagonal 647, Barcelona, E-08028, Spain. carlos.aleman@upc.edu.|Centre for Research in Nano-Engineering, Universitat Politècnica de Catalunya, Edifici C', C/Pasqual i Vila s/n, Barcelona, E-08028, Spain. carlos.aleman@upc.edu.
authors.forename: Carlos
authors.initials: C
authors.lastname: Alemán
mesh_terms: D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry*; D002448:Cell Adhesion; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D005260:Female; D005337:Fibrin / Q000737:chemistry*; D006801:Humans; D061986:MCF-7 Cells; D008297:Male; D008567:Membranes, Artificial; D009362:Neoplasm Metastasis; D009842:Oligopeptides / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D013876:Thiophenes / Q000737:chemistry*; D013917:Thrombin / Q000737:chemistry
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D020099:Coated Materials, Biocompatible; D008567:Membranes, Artificial; D009842:Oligopeptides; D011108:Polymers; D013876:Thiophenes; C542438:cysteinyl-arginyl-glutamyl-lysyl-alanyl; C121383:poly(3,4-ethylene dioxythiophene); C066730:polythiophene; D005337:Fibrin; D013917:Thrombin
keywords: cell differentiation; electroactive polymers; fibrin adsorption; peptide-polymer composites; poly(3,4-ethylenedi-oxythiophene)