title: Circulating Extracellular Vesicles Contain miRNAs and are Released as Early Biomarkers for Cardiac Injury.
abstract: Plasma-circulating microRNAs have been implicated as novel early biomarkers for myocardial infarction (MI) due to their high specificity for cardiac injury. For swift clinical translation of this potential biomarker, it is important to understand their temporal and spatial characteristics upon MI. Therefore, we studied the temporal release, potential source, and transportation of circulating miRNAs in different models of ischemia reperfusion (I/R) injury. We demonstrated that extracellular vesicles are released from the ischemic myocardium upon I/R injury. Moreover, we provided evidence that cardiac and muscle-specific miRNAs are transported by extracellular vesicles and are rapidly detectable in plasma. Since these vesicles are enriched for the released miRNAs and their detection precedes traditional damage markers, they hold great potential as specific early biomarkers for MI.
authors.affiliation: Department of Cardiology, University Medical Center Utrecht, Utrecht, The Netherlands.|Netherlands Heart Institute (ICIN), Utrecht, The Netherlands.
authors.forename: Janine C
authors.initials: JC
authors.lastname: Deddens
authors.affiliation: Department of Cardiology, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Krijn R
authors.initials: KR
authors.lastname: Vrijsen
authors.affiliation: Department of Cardiology, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Johanna M
authors.initials: JM
authors.lastname: Colijn
authors.affiliation: Department of Cardiology, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Martinus I
authors.initials: MI
authors.lastname: Oerlemans
authors.affiliation: Department of Cardiology, University Medical Center Utrecht, Utrecht, The Netherlands.|UMC Utrecht Regenerative Medicine Center, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Corina H G
authors.initials: CH
authors.lastname: Metz
authors.affiliation: Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
authors.forename: Els J
authors.initials: EJ
authors.lastname: van der Vlist
authors.affiliation: Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
authors.forename: Esther N M
authors.initials: EN
authors.lastname: Nolte-'t Hoen
authors.affiliation: UMC Utrecht Regenerative Medicine Center, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Krista
authors.initials: K
authors.lastname: den Ouden
authors.affiliation: Department of Cardiology, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Sanne J
authors.initials: SJ
authors.lastname: Jansen Of Lorkeers
authors.affiliation: Department of Cardiology, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Tycho I G
authors.initials: TI
authors.lastname: van der Spoel
authors.affiliation: Department of Cardiology, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Stefan
authors.initials: S
authors.lastname: Koudstaal
authors.affiliation: Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
authors.forename: Ger J
authors.initials: GJ
authors.lastname: Arkesteijn
authors.affiliation: Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
authors.forename: Marca H M
authors.initials: MH
authors.lastname: Wauben
authors.affiliation: Department of Cardiology, University Medical Center Utrecht, Utrecht, The Netherlands.|UMC Utrecht Regenerative Medicine Center, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Linda W
authors.initials: LW
authors.lastname: van Laake
authors.affiliation: Department of Cardiology, University Medical Center Utrecht, Utrecht, The Netherlands.|UMC Utrecht Regenerative Medicine Center, University Medical Center Utrecht, Utrecht, The Netherlands.|Netherlands Heart Institute (ICIN), Utrecht, The Netherlands.
authors.forename: Pieter A
authors.initials: PA
authors.lastname: Doevendans
authors.affiliation: Department of Cardiology, University Medical Center Utrecht, Utrecht, The Netherlands.|UMC Utrecht Regenerative Medicine Center, University Medical Center Utrecht, Utrecht, The Netherlands.|Netherlands Heart Institute (ICIN), Utrecht, The Netherlands.
authors.forename: Steven A J
authors.initials: SA
authors.lastname: Chamuleau
authors.affiliation: Department of Cardiology, University Medical Center Utrecht, Utrecht, The Netherlands. J.Sluijter@umcutrecht.nl.|UMC Utrecht Regenerative Medicine Center, University Medical Center Utrecht, Utrecht, The Netherlands. J.Sluijter@umcutrecht.nl.|Netherlands Heart Institute (ICIN), Utrecht, The Netherlands. J.Sluijter@umcutrecht.nl.|Department of Cardiology, Experimental Cardiology Laboratory, University Medical Center Utrecht, Heidelberglaan 100, PO Box 85500, 3508 GA, Utrecht, The Netherlands. J.Sluijter@umcutrecht.nl.
authors.forename: Joost P G
authors.initials: JP
authors.lastname: Sluijter
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D000067128:Extracellular Vesicles / Q000378:metabolism*; D005260:Female; D005819:Genetic Markers; D000067268:Isolated Heart Preparation; D008297:Male; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D009203:Myocardial Infarction / Q000097:blood / Q000175:diagnosis / Q000235:genetics*; D015428:Myocardial Reperfusion Injury / Q000097:blood / Q000175:diagnosis / Q000235:genetics*; D034421:Sus scrofa; D013997:Time Factors
chemical_list: D005819:Genetic Markers; D035683:MicroRNAs
keywords: Biomarkers; Circulating microRNA; Exosomes; Extracellular vesicles; Myocardial infarction