title: Highly solvatochromic and tunable fluorophores based on a 4,5-quinolimide scaffold: novel CDK5 probes.
abstract: Novel 4,5-quinolimide-based fluorophores are more solvatochromic and red-shifted than known naphthalimide analogues. Conjugation of one of these fluorophores to a peptide derived from CDK5 kinase demonstrated its sensitivity for monitoring the interaction with its regulatory partner p25. Introduction of the quinolimide-labelled peptide into living glioblastoma cells probed the interaction with endogenous p25.
authors.affiliation: Instituto de Química Médica (CSIC), Juan de la Cierva 3, 28006 Madrid, Spain. rosario@iqm.csic.es.
authors.forename: Juan A
authors.initials: JA
authors.lastname: González-Vera
authors.forename: Francisco
authors.initials: F
authors.lastname: Fueyo-González
authors.forename: Ibon
authors.initials: I
authors.lastname: Alkorta
authors.forename: Marion
authors.initials: M
authors.lastname: Peyressatre
authors.forename: May C
authors.initials: MC
authors.lastname: Morris
authors.forename: Rosario
authors.initials: R
authors.lastname: Herranz
mesh_terms: D051360:Cyclin-Dependent Kinase 5 / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D015394:Molecular Structure; D011804:Quinolines / Q000138:chemical synthesis / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry
chemical_list: C000626187:4,5-quinolimide; D005456:Fluorescent Dyes; D011804:Quinolines; D012997:Solvents; D051360:Cyclin-Dependent Kinase 5; C495905:CDK5 protein, human