title: First detection of Apis mellifera filamentous virus in Apis cerana cerana in China.
abstract: Although many honey bee RNA viruses have been correlated with colony declines, little is known regarding the potential role of DNA viruses. Here, we examined seemingly healthy and crawling bee samples from China using PCR to identify whether Apis mellifera filamentous virus (AmFV) was present in A. cerana cerana. The highest AmFV infection percentage among Chinese provinces occurred in crawling bees from Gansu province (85.48%), and the lowest was in bees from Beijing (31.58%). A phylogenetic analysis showed that the Chinese isolate of AmFV exhibited a high genetic similarity with isolates from Belgium, Switzerland and USA. This is the first report of AmFV infections in Chinese A. cerana cerana populations.
authors.affiliation: Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, PR China; Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Beijing 100093, PR China.
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Hou
authors.affiliation: Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, PR China; Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Beijing 100093, PR China; Graduate School of the Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Beibei
authors.initials: B
authors.lastname: Li
authors.affiliation: Guangdong Entomological Institute, Guangzhou 510260, PR China.
authors.forename: Yuexiong
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, PR China; Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Beijing 100093, PR China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Deng
authors.affiliation: Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, PR China; Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Beijing 100093, PR China. Electronic address: dqyun1@126.com.
authors.forename: Qingyun
authors.initials: Q
authors.lastname: Diao
mesh_terms: D000818:Animals; D001516:Bees / Q000821:virology*; D002681:China; D004267:DNA Viruses; D005814:Genes, Viral / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D014777:Virus Diseases / Q000662:veterinary*
keywords: Apis cerana cerana; DNA virus; Filamentous virus