title: Comparison of gas chromatography-combustion-mass spectrometry and gas chromatography-flame ionization detector for the determination of fatty acid methyl esters in biodiesel without specific standards.
abstract: GC-FID has been effectively used as a universal quantification technique for volatile organic compounds for a long time. In most cases, the use of the ECN allows for quantification by GC-FID without external calibration using only the response of a single internal standard. In this paper we compare the performance characteristics of GC-FID with those of post-column (13)C Isotope Dilution GC-Combustion-MS for the absolute quantification of organic compounds without the need for individual standards. For this comparison we have selected the quantification of FAMEs in biodiesel. The selection of the right internal standard was critical for GC-FID even when ECN were considered. On the other hand, the nature of the internal standard was not relevant when GC-Combustion-MS was employed. The proposed method was validated with the analysis of the certified reference material SRM 2772 and comparative data was obtained on real biodiesel samples. The analysis of the SRM 2772 biodiesel provided recoveries in the range 100.6-103.5% and 96.4-103.6% for GC-combustion-MS and GC-FID, respectively. The detection limit for GC-combustion-MS was found to be 4.2ng compound/g of injected sample. In conclusion, the quantitative performance of GC-Combustion-MS compared satisfactorily with that of GC-FID constituting a viable alternative for the quantification of organic compounds without the need for individual standards.
authors.affiliation: Department of Physical and Analytical Chemistry, University of Oviedo, Julián Clavería 8, 33006, Oviedo, Spain.
authors.forename: Laura Alonso
authors.initials: LA
authors.lastname: Sobrado
authors.affiliation: Department of Physical and Analytical Chemistry, University of Oviedo, Julián Clavería 8, 33006, Oviedo, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Freije-Carrelo
authors.affiliation: Department of Physical and Analytical Chemistry, University of Oviedo, Julián Clavería 8, 33006, Oviedo, Spain.
authors.forename: Mariella
authors.initials: M
authors.lastname: Moldovan
authors.affiliation: Department of Physical and Analytical Chemistry, University of Oviedo, Julián Clavería 8, 33006, Oviedo, Spain. Electronic address: ruizencinar@uniovi.es.
authors.forename: Jorge Ruiz
authors.initials: JR
authors.lastname: Encinar
authors.affiliation: Department of Physical and Analytical Chemistry, University of Oviedo, Julián Clavería 8, 33006, Oviedo, Spain. Electronic address: jiga@uniovi.es.
authors.forename: J Ignacio García
authors.initials: JI
authors.lastname: Alonso
mesh_terms: D056804:Biofuels / Q000032:analysis*; D002138:Calibration; D002849:Chromatography, Gas / Q000379:methods; D004952:Esters; D005227:Fatty Acids / Q000032:analysis*; D005410:Flame Ionization; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D057230:Limit of Detection; D055549:Volatile Organic Compounds / Q000032:analysis*
chemical_list: D056804:Biofuels; D004952:Esters; D005227:Fatty Acids; D055549:Volatile Organic Compounds
keywords: Absolute quantification; Biodiesel; FAMEs; GC-FID; GC-combustion-MS; Internal standard