title: Click Chemistry Immobilization of Antibodies on Polymer Coated Gold Nanoparticles.
abstract: The goal of this work is to develop an innovative approach for the coating of gold nanoparticles (AuNPs) with a synthetic functional copolymer. This stable coating with a thickness of few nanometers provides, at the same time, stabilization and functionalization of the particles. The polymeric coating consists of a backbone of polydimethylacrylamide (DMA) functionalized with an alkyne monomer that allows the binding of azido modified molecules by Cu(I)-catalyzed azide/alkyne 1,3-dipolar cycloaddition (CuAAC, click chemistry). The thin polymer layer on the surface stabilizes the colloidal suspension whereas the alkyne functions pending from the backbone are available for the reaction with azido-modified proteins. The reactivity of the coating is demonstrated by immobilizing an azido modified anti-mouse IgG antibody on the particle surface. This approach for the covalent binding of antibody to a gold-NPs is applied to the development of gold labels in biosensing techniques.
authors.affiliation: Istituto di Chimica del Riconoscimento Molecolare, CNR , Via Mario Bianco 9, 20131 Milano, Italy.|Dipartimento di Scienze Farmaceutiche, Università degli Studi di Milano , Via Mangiagalli 25, 20133 Milano, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Finetti
authors.affiliation: Istituto di Chimica del Riconoscimento Molecolare, CNR , Via Mario Bianco 9, 20131 Milano, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Sola
authors.forename: Margherita
authors.initials: M
authors.lastname: Pezzullo
authors.affiliation: NanobioLab, Dipartimento di Biotecnologie e Bioscienze, Università degli Studi di Milano-Bicocca , Piazza della Scienza 2, 20126 Milano, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Prosperi
authors.affiliation: NanobioLab, Dipartimento di Biotecnologie e Bioscienze, Università degli Studi di Milano-Bicocca , Piazza della Scienza 2, 20126 Milano, Italy.
authors.forename: Miriam
authors.initials: M
authors.lastname: Colombo
authors.affiliation: NanobioLab, Dipartimento di Biotecnologie e Bioscienze, Università degli Studi di Milano-Bicocca , Piazza della Scienza 2, 20126 Milano, Italy.
authors.forename: Benedetta
authors.initials: B
authors.lastname: Riva
authors.affiliation: NanobioLab, Dipartimento di Biotecnologie e Bioscienze, Università degli Studi di Milano-Bicocca , Piazza della Scienza 2, 20126 Milano, Italy.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Avvakumova
authors.affiliation: Laboratory of Nanomedicine and Clinical Biophotonics, Centre for Innovation and Technology Transfer, Fondazione Don Carlo Gnocchi ONLUS , 20121 Milano, Italy.
authors.forename: Carlo
authors.initials: C
authors.lastname: Morasso
authors.affiliation: Laboratory of Nanomedicine and Clinical Biophotonics, Centre for Innovation and Technology Transfer, Fondazione Don Carlo Gnocchi ONLUS , 20121 Milano, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Picciolini
authors.affiliation: Istituto di Chimica del Riconoscimento Molecolare, CNR , Via Mario Bianco 9, 20131 Milano, Italy.
authors.forename: Marcella
authors.initials: M
authors.lastname: Chiari
mesh_terms: D000178:Acrylamides / Q000737:chemistry; D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry*; D003102:Colloids; D003300:Copper / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D011817:Rabbits
chemical_list: D000178:Acrylamides; D055910:Antibodies, Immobilized; D003102:Colloids; D007074:Immunoglobulin G; C429790:poly(N,N-dimethylacrylamide); D006046:Gold; D003300:Copper