title: Dihydroceramide-desaturase-1-mediated caspase 9 activation through ceramide plays a pivotal role in palmitic acid-induced HepG2 cell apoptosis.
abstract: In this study, results showed that the inhibition of PA-induced HepG2 cell growth takes place in a time- and concentration-dependent manner, that activation of caspase 9 is necessary for PA-induced HepG2 cell apoptosis, that dihydroceramide desaturase 1 (DES1) plays a key role in PA-mediated caspase 9 and caspase 3 activation, and that palmitoleic acid (POA), an omega-7 monounsaturated fatty acid, reverses PA-induced apoptosis through DES1 → Ceramide → Caspase 9 → Caspase 3 signaling.
authors.affiliation: Laboratory of Hepatobiliary and Pancreatic Surgery, Affiliated Hospital of Guilin Medical University, Guilin, 541001, Guangxi, People's Republic of China.|Department of Endocrinology, The Second Affiliated Hospital of Nanjing Medical University, 210011, Nanjing, Jiangsu, People's Republic of China.|China-USA Lipids in Health and Disease Research Center, Guilin Medical University, Guilin, 541001, Guangxi, People's Republic of China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Zhu
authors.affiliation: Laboratory of Hepatobiliary and Pancreatic Surgery, Affiliated Hospital of Guilin Medical University, Guilin, 541001, Guangxi, People's Republic of China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Yang
authors.affiliation: Laboratory of Hepatobiliary and Pancreatic Surgery, Affiliated Hospital of Guilin Medical University, Guilin, 541001, Guangxi, People's Republic of China.
authors.forename: Rongping
authors.initials: R
authors.lastname: Zhu
authors.affiliation: Guangxi Key Laboratory of Molecular Medicine in Liver Injury and Repair, Guilin Medical University, Guilin, 541001, Guangxi, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Guangxi Key Laboratory of Molecular Medicine in Liver Injury and Repair, Guilin Medical University, Guilin, 541001, Guangxi, People's Republic of China.
authors.forename: Wanlian
authors.initials: W
authors.lastname: Li
authors.affiliation: Laboratory of Hepatobiliary and Pancreatic Surgery, Affiliated Hospital of Guilin Medical University, Guilin, 541001, Guangxi, People's Republic of China. dr_hesongqing@163.com.|Guangxi Key Laboratory of Molecular Medicine in Liver Injury and Repair, Guilin Medical University, Guilin, 541001, Guangxi, People's Republic of China. dr_hesongqing@163.com.
authors.forename: Songqing
authors.initials: S
authors.lastname: He
authors.affiliation: Laboratory of Hepatobiliary and Pancreatic Surgery, Affiliated Hospital of Guilin Medical University, Guilin, 541001, Guangxi, People's Republic of China. changliangzijin@163.com.|China-USA Lipids in Health and Disease Research Center, Guilin Medical University, Guilin, 541001, Guangxi, People's Republic of China. changliangzijin@163.com.|Guangxi Key Laboratory of Molecular Medicine in Liver Injury and Repair, Guilin Medical University, Guilin, 541001, Guangxi, People's Republic of China. changliangzijin@163.com.
authors.forename: Junfei
authors.initials: J
authors.lastname: Jin
mesh_terms: D017209:Apoptosis; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D053453:Caspase 9 / Q000235:genetics / Q000378:metabolism*; D002518:Ceramides / Q000378:metabolism*; D004789:Enzyme Activation; D056945:Hep G2 Cells; D006801:Humans; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D019308:Palmitic Acid / Q000378:metabolism*
chemical_list: D002518:Ceramides; D019308:Palmitic Acid; D010088:Oxidoreductases; C108138:dihydroceramide desaturase; D053148:Caspase 3; D053453:Caspase 9
keywords: Caspase 9; Cell apoptosis; Ceramide; Dihydroceramide desaturase 1; Palmitic acid; Palmitoleic acid