title: Efficient and safe gene delivery to human corneal endothelium using magnetic nanoparticles.
abstract: To develop a safe and efficient method for targeted, anti-apoptotic gene therapy of corneal endothelial cells (CECs).
  
  
  
  Magnetofection (MF), a combination of lipofection with magnetic nanoparticles (MNPs; PEI-Mag2, SO-Mag5, PalD1-Mag1), was tested in human CECs and in explanted human corneas. Effects on cell viability and function were investigated. Immunocompatibility was assessed in human peripheral blood mononuclear cells.
  
  
  
  Silica iron-oxide MNPs (SO-Mag5) combined with X-tremeGENE-HP achieved high transfection efficiency in human CECs and explanted human corneas, without altering cell viability or function. Magnetofection caused no immunomodulatory effects in human peripheral blood mononuclear cells. Magnetofection with anti-apoptotic P35 gene effectively blocked apoptosis in CECs.
  
  
  
  Magnetofection is a promising tool for gene therapy of corneal endothelial cells with potential for targeted on-site delivery.
authors.affiliation: Department of Ophthalmology, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany.|Institute of Anatomy, University Duisburg-Essen, Essen, Germany.
authors.forename: Marta
authors.initials: M
authors.lastname: Czugala
authors.affiliation: Institute of Immunology & Experimental Oncology, Klinikum rechts der Isar, Technische Universität München, Munich, Germany.
authors.forename: Olga
authors.initials: O
authors.lastname: Mykhaylyk
authors.affiliation: Institute of Molecular Medicine I, Heinrich Heine University Düsseldorf, Düsseldorf, Germany.
authors.forename: Philip
authors.initials: P
authors.lastname: Böhler
authors.affiliation: Department of Ophthalmology, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany.
authors.forename: Jasmine
authors.initials: J
authors.lastname: Onderka
authors.affiliation: Institute of Molecular Medicine I, Heinrich Heine University Düsseldorf, Düsseldorf, Germany.
authors.forename: Björn
authors.initials: B
authors.lastname: Stork
authors.affiliation: Institute of Molecular Medicine I, Heinrich Heine University Düsseldorf, Düsseldorf, Germany.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Wesselborg
authors.affiliation: Department of Ophthalmology, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany.
authors.forename: Friedrich E
authors.initials: FE
authors.lastname: Kruse
authors.affiliation: Institute of Immunology & Experimental Oncology, Klinikum rechts der Isar, Technische Universität München, Munich, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Plank
authors.affiliation: Institute of Anatomy, University Duisburg-Essen, Essen, Germany.
authors.forename: Bernhard B
authors.initials: BB
authors.lastname: Singer
authors.affiliation: Department of Ophthalmology, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany.
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Fuchsluger
mesh_terms: D015703:Antigens, CD / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D002460:Cell Line; D045744:Cell Line, Tumor; D002470:Cell Survival; D004247:DNA / Q000008:administration & dosage / Q000235:genetics; D004728:Endothelium, Corneal / Q000378:metabolism*; D006801:Humans; D053645:Interleukin-2 Receptor alpha Subunit / Q000032:analysis; D037181:Lectins, C-Type / Q000032:analysis; D007963:Leukocytes, Mononuclear / Q000166:cytology; D060526:Magnetic Fields; D008280:Magnetics / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D010957:Plasmids / Q000008:administration & dosage / Q000235:genetics; D012822:Silicon Dioxide / Q000737:chemistry*; D014162:Transfection / Q000379:methods*; D014764:Viral Proteins / Q000235:genetics; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D004247:DNA; D053645:Interleukin-2 Receptor alpha Subunit; D037181:Lectins, C-Type; D058185:Magnetite Nanoparticles; D012822:Silicon Dioxide; D014764:Viral Proteins; D000099192:CD69 Antigens; C492234:p35 protein, Baculovirus
keywords: apoptosis; corneal endothelium; gene therapy; magnetic nanoparticles; magnetofection