title: Improvement in methanol production by regulating the composition of synthetic gas mixture and raw biogas.
abstract: Raw biogas can be an alternative feedstock to pure methane (CH4) for methanol production. In this investigation, we evaluated the methanol production potential of Methylosinus sporium from raw biogas originated from an anaerobic digester. Furthermore, the roles of different gases in methanol production were investigated using synthetic gas mixtures of CH4, carbon dioxide (CO2), and hydrogen (H2). Maximum methanol production was 5.13, 4.35, 6.28, 7.16, 0.38, and 0.36mM from raw biogas, CH4:CO2, CH4:H2, CH4:CO2:H2, CO2, and CO2:H2, respectively. Supplementation of H2 into raw biogas increased methanol production up to 3.5-fold. Additionally, covalent immobilization of M. sporium on chitosan resulted in higher methanol production from raw biogas. This study provides a suitable approach to improve methanol production using low cost raw biogas as a feed containing high concentrations of H2S (0.13%). To our knowledge, this is the first report on methanol production from raw biogas, using immobilized cells of methanotrophs.
authors.affiliation: Department of Chemical Engineering, Konkuk University, Seoul 05029, Republic of Korea.
authors.forename: Sanjay K S
authors.initials: SK
authors.lastname: Patel
authors.affiliation: Department of Chemical Engineering, Konkuk University, Seoul 05029, Republic of Korea.
authors.forename: Primata
authors.initials: P
authors.lastname: Mardina
authors.affiliation: Phygen Research Institute, Daejeon 34325, Republic of Korea.
authors.forename: Dongwook
authors.initials: D
authors.lastname: Kim
authors.affiliation: Department of Food Science & Biotechnology, Shin-Ansan University, Ansan 15435, Republic of Korea.
authors.forename: Sang-Yong
authors.initials: SY
authors.lastname: Kim
authors.affiliation: CSIR-Institute of Genomics and Integrative Biology (IGIB), Delhi University Campus, Mall Road, Delhi 110 007, India.
authors.forename: Vipin C
authors.initials: VC
authors.lastname: Kalia
authors.affiliation: Department of Chemical Engineering, Konkuk University, Seoul 05029, Republic of Korea.
authors.forename: In-Won
authors.initials: IW
authors.lastname: Kim
authors.affiliation: Department of Chemical Engineering, Konkuk University, Seoul 05029, Republic of Korea. Electronic address: jkrhee@konkuk.ac.kr.
authors.forename: Jung-Kul
authors.initials: JK
authors.lastname: Lee
mesh_terms: D000693:Anaerobiosis; D056804:Biofuels; D001709:Biotechnology; D002245:Carbon Dioxide / Q000737:chemistry*; D005740:Gases; D006859:Hydrogen / Q000737:chemistry*; D008697:Methane / Q000737:chemistry*; D000432:Methanol / Q000737:chemistry*; D020581:Methylosinus
chemical_list: D056804:Biofuels; D005740:Gases; D002245:Carbon Dioxide; D006859:Hydrogen; D008697:Methane; D000432:Methanol
keywords: Anaerobic digester biogas; Methane; Methanol; Methylosinus sporium; Whole cell immobilization