title: A Microbiome DNA Enrichment Method for Next-Generation Sequencing Sample Preparation.
abstract: "Microbiome" is used to describe the communities of microorganisms and their genes in a particular environment, including communities in association with a eukaryotic host or part of a host. One challenge in microbiome analysis concerns the presence of host DNA in samples. Removal of host DNA before sequencing results in greater sequence depth of the intended microbiome target population. This unit describes a novel method of microbial DNA enrichment in which methylated host DNA such as human genomic DNA is selectively bound and separated from microbial DNA before next-generation sequencing (NGS) library construction. This microbiome enrichment technique yields a higher fraction of microbial sequencing reads and improved read quality resulting in a reduced cost of downstream data generation and analysis. © 2016 by John Wiley & Sons, Inc.
authors.affiliation: New England Biolabs, Ipswich, Massachusetts.
authors.forename: Erbay
authors.initials: E
authors.lastname: Yigit
authors.affiliation: New England Biolabs, Ipswich, Massachusetts.
authors.forename: George R
authors.initials: GR
authors.lastname: Feehery
authors.affiliation: New England Biolabs, Ipswich, Massachusetts.
authors.forename: Bradley W
authors.initials: BW
authors.lastname: Langhorst
authors.affiliation: New England Biolabs, Ipswich, Massachusetts.
authors.forename: Fiona J
authors.initials: FJ
authors.lastname: Stewart
authors.affiliation: New England Biolabs, Ipswich, Massachusetts.
authors.forename: Eileen T
authors.initials: ET
authors.lastname: Dimalanta
authors.affiliation: New England Biolabs, Ipswich, Massachusetts.
authors.forename: Sriharsa
authors.initials: S
authors.lastname: Pradhan
authors.affiliation: New England Biolabs, Ipswich, Massachusetts.
authors.forename: Barton
authors.initials: B
authors.lastname: Slatko
authors.affiliation: New England Biolabs, Ipswich, Massachusetts.
authors.forename: Andrew F
authors.initials: AF
authors.lastname: Gardner
authors.affiliation: New England Biolabs, Ipswich, Massachusetts.
authors.forename: James
authors.initials: J
authors.lastname: McFarland
authors.affiliation: New England Biolabs, Ipswich, Massachusetts.
authors.forename: Christine
authors.initials: C
authors.lastname: Sumner
authors.affiliation: New England Biolabs, Ipswich, Massachusetts.
authors.forename: Theodore B
authors.initials: TB
authors.lastname: Davis
mesh_terms: D011232:Chemical Precipitation; D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D019175:DNA Methylation; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D064307:Microbiota; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D004247:DNA
keywords: DNA sequencing; NGG; metagenomics DNA enrichment; methylation; microbiome; next-generation sequencing; target enrichment