title: Effect of crude glycerol impurities on lipid preparation by Rhodosporidium toruloides yeast 32489.
abstract: Crude glycerol (byproduct of biodiesel preparation) was utilised as the carbon source to produce lipid using oleaginous yeast Rhodosporidium toruloides 32489. Under the same conditions, lipid production with crude glycerol was higher than those produced with glucose and pure glycerol. The effects of 4 main impurities in crude glycerol (methyl oleate, sodium oleate, NaCl and methanol) on lipid production were investigated. Compared with utilising pure glycerol, addition of methyl oleate, sodium oleate, and NaCl impurities increased lipid production by 47.0%, 68.0% and 64.0%, respectively, while methanol decreased lipid production by 17.7%. However, when methanol was mixed with other impurities, its inhibition effect was alleviated due to the promoting effect of other impurities. Hence, crude glycerol could be used as a renewable and low-cost carbon source to replace pure glucose or glycerol for lipid preparation.
authors.affiliation: Department of Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Gao
authors.affiliation: Advanced Environmental Biotechnology Centre, Nanyang Environment & Water Research Institute, Nanyang Technological University, 1 Cleantech Loop, Singapore 637141, Singapore.
authors.forename: Yingqun
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, China; Beijing Key Laboratory on Resource-oriented Treatment of Industrial Pollutants, China. Electronic address: wangqh59@sina.com.
authors.forename: Qunhui
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.affiliation: Advanced Environmental Biotechnology Centre, Nanyang Environment & Water Research Institute, Nanyang Technological University, 1 Cleantech Loop, Singapore 637141, Singapore.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
mesh_terms: D001487:Basidiomycota; D056804:Biofuels; D018533:Biomass; D005947:Glucose / Q000737:chemistry; D005990:Glycerol / Q000737:chemistry*; D012441:Saccharomyces cerevisiae
chemical_list: D056804:Biofuels; D005947:Glucose; D005990:Glycerol
keywords: Crude glycerol; Impurities; Lipid production; Rhodosporidium toruloides yeast 32489