title: [Silencing of ezrin gene inhibits proliferation and invasion of human prostate cancer PC-3 cells].
abstract: Objective To observe the expression of ezrin in prostate cancer tissues and explore the effect of ezrin gene silencing on the proliferation and invasion of human prostate cancer PC-3 cells and involved mechanisms. Methods The paraffin-embedded specimens of prostate cancer (n=20) and benign prostatic hyperplasia (n=20) were collected. Real-time quantitative PCR and Western blotting were used to detect the expression of ezrin mRNA and protein in prostate tissues, respectively. After culturing, human prostate cancer PC-3 cells were treated with culture solution, Scramble siRNA and ezrin siRNA for 48 hours. Cell proliferation was analyzed through MTT method. Cell invasion ability was measured using Transwell(TM) invasion experiment. In addition, the levels of E-cadherin and N-cadherin were examined by real-time quantitative PCR and Western blotting. Results The expression levels of ezrin mRNA and protein in prostate cancer tissues were higher than those in the tissues from benign prostatic hyperplasia patients. In comparison with PC-3 cells treated with Scramble siRNA, following the downregulation of ezrin expression, PC-3 cell proliferation was significantly inhibited and mean cell number of penetrated membrane and N-cadherin level were reduced, whereas E-cadherin level was raised. There was no statistical difference in the above indicators between Scramble siRNA-treated PC-3 cells and pure culture solution-treated PC-3 cells. Conclusion Ezrin is highly expressed in prostate cancer tissues. Gene silence of ezrin inhibits the proliferation and invasion of human prostate cancer PC-3 cells, meanwhile the level of E-cadherin is upregulated and N-cadherin is downregulated.
authors.affiliation: Department of Urology, Second Affiliated Hospital, University of South China, Hengyang 421001, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Yang
authors.affiliation: Hunan University of Environment and Biology, Hengyang 421001, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Urology, Second Affiliated Hospital, University of South China, Hengyang 421001, China.
authors.forename: Xian
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Urology, Second Affiliated Hospital, University of South China, Hengyang 421001, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Urology, Second Affiliated Hospital, University of South China, Hengyang 421001, China. *Corresponding author, E-mail: luo6398@sina.com.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Luo
mesh_terms: D015153:Blotting, Western; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D003598:Cytoskeletal Proteins / Q000235:genetics* / Q000378:metabolism; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D009361:Neoplasm Invasiveness; D011470:Prostatic Hyperplasia / Q000235:genetics / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation; D000099149:Ezrin
chemical_list: D015820:Cadherins; D003598:Cytoskeletal Proteins; D000099149:Ezrin