title: Human mesenchymal stromal cell-enhanced osteogenic differentiation by contact interaction with polyethylene terephthalate nanogratings.
abstract: Among the very large number of polymeric materials that have been proposed in the field of orthopedics, polyethylene terephthalate (PET) is one of the most attractive thanks to its flexibility, thermal resistance, mechanical strength and durability. Several studies have been proposed that interface nano- or micro-structured surfaces with mesenchymal stromal cells (MSCs), demonstrating the potential of this technology for promoting osteogenesis. All these studies were carried out on biomaterials other than PET, which remains almost uninvestigated in terms of cell shaping, alignment and differentiation. Here, we study the effect of PET 350-depth nanogratings (NGs) with a ridge and lateral groove size of 500 nm (T1) or 1 μm (T2), on bone marrow-derived human MSC (hMSC) differentiation in relation to the osteogenic fate. We demonstrate that these substrates, especially T2, can promote the osteogenic phenotype more efficiently than standard flat surfaces and that this effect is more marked if cells are cultured in osteogenic medium than in basal medium. Finally, we show that the shape and disposition of calcium hydroxyapatite granules on the different substrates was influenced by the substrate symmetry, being more elongated and spatially organized on NGs than on flat surfaces.
authors.affiliation: NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, Piazza San Silvestro 12, Pisa 56127, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Antonini
authors.forename: Sandro
authors.initials: S
authors.lastname: Meucci
authors.forename: Paolo
authors.initials: P
authors.lastname: Parchi
authors.forename: Simone
authors.initials: S
authors.lastname: Pacini
authors.forename: Marina
authors.initials: M
authors.lastname: Montali
authors.forename: Andrea
authors.initials: A
authors.lastname: Poggetti
authors.forename: Michele
authors.initials: M
authors.lastname: Lisanti
authors.forename: Marco
authors.initials: M
authors.lastname: Cecchini
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D017886:Durapatite; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D049329:Nanostructures / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D010012:Osteogenesis / Q000502:physiology*; D011093:Polyethylene Terephthalates / Q000737:chemistry / Q000378:metabolism*; D054457:Tissue Scaffolds
chemical_list: D011093:Polyethylene Terephthalates; D017886:Durapatite