title: Downregulated long non-coding RNA CLMAT3 promotes the proliferation of colorectal cancer cells by targeting regulators of the cell cycle pathway.
abstract: Over-expression of long non-coding RNA (lncRNA)-CLMAT3 is significantly associated with colorectal liver metastasis and is an independent predictor of poor survival for colorectal cancer patients. However, as little is known regarding the role of this gene in the proliferation of colorectal cancer in vitro, we investigated the involvement of lncRNA-CLMAT3 in colorectal cancer cell proliferation. In this study, we demonstrate that lncRNA-CLMAT3 expression was significantly increased in colorectal cancer cells compared with a normal intestinal mucous cell line and that inhibition of lncRNA-CLMAT3 suppressed colorectal cancer cell proliferation in vitro. We also found that this reduced colorectal cancer cell proliferation due to lncRNA-CLMAT3 knockdown is associated with G0/G1 cell-cycle arrest induction and apoptosis enhancement. Furthermore, lncRNA-CLMAT3 knockdown enhanced Cdh1 expression and resulted in p27Kip accumulation via increased Skp2 protein ubiquitination. Taken together, our findings suggest that reducing lncRNA-CLMAT3 inhibits colorectal cancer cell proliferation by affecting cell cycle components.
authors.affiliation: Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, P.R. China.|Department of Oncological Surgery, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, P.R. China.
authors.forename: Le-Chi
authors.initials: LC
authors.lastname: Ye
authors.affiliation: Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.affiliation: Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, P.R. China.
authors.forename: De-Xiang
authors.initials: DX
authors.lastname: Zhu
authors.affiliation: Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, P.R. China.
authors.forename: Shi-Xu
authors.initials: SX
authors.lastname: Lv
authors.affiliation: Department of Gynecology, Obstetrics and Gynecology Hospital of Fudan University, Shanghai, P.R. China.
authors.forename: Jun-Jun
authors.initials: JJ
authors.lastname: Qiu
authors.affiliation: Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, P.R. China.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Xu
authors.affiliation: The Third Hospital Affiliated to Nantong University, Nantong, P.R. China.
authors.forename: Feng-Lai
authors.initials: FL
authors.lastname: Yuan
authors.affiliation: Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, P.R. China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Wei
mesh_terms: D015703:Antigens, CD; D017209:Apoptosis; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D002453:Cell Cycle; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000628:therapy; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000378:metabolism; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000473:pathology; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D014407:Tumor Cells, Cultured; D054875:Ubiquitination
chemical_list: D015703:Antigens, CD; C513384:CDH1 protein, human; C436291:CDKN1B protein, human; C000606574:CLMAT3 long noncoding RNA, human; D015820:Cadherins; D062085:RNA, Long Noncoding; D034741:RNA, Small Interfering; D050760:Cyclin-Dependent Kinase Inhibitor p27
keywords: cell cycle pathway; colorectal cancer; lncRNA-CLMAT3; long non-coding RNA