title: Neutron and X-ray single-crystal diffraction from protein microcrystals via magnetically oriented microcrystal arrays in gels.
abstract: Protein microcrystals magnetically aligned in D2O hydrogels were subjected to neutron diffraction measurements, and reflections were observed for the first time to a resolution of 3.4 Å from lysozyme microcrystals (∼10 × 10 × 50 µm). This result demonstrated the possibility that magnetically oriented microcrystals consolidated in D2O gels may provide a promising means to obtain single-crystal neutron diffraction from proteins that do not crystallize at the sizes required for neutron diffraction structure determination. In addition, lysozyme microcrystals aligned in H2O hydrogels allowed structure determination at a resolution of 1.76 Å at room temperature by X-ray diffraction. The use of gels has advantages since the microcrystals are measured under hydrated conditions.
authors.affiliation: Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Shu
authors.initials: S
authors.lastname: Tsukui
authors.affiliation: Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Fumiko
authors.initials: F
authors.lastname: Kimura
authors.affiliation: Frontier Research Center for Applied Atomic Sciences, Ibaraki University, 162-1 Shirakata, Tokai, Ibaraki 319-1106, Japan.
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Kusaka
authors.affiliation: Structural Biology Group, Japan Synchrotron Radiation Research Institute (JASRI/SPring-8), 1-1-1 Kouto, Sayo, Hyogo 679-5198, Japan.
authors.forename: Seiki
authors.initials: S
authors.lastname: Baba
authors.affiliation: Structural Biology Group, Japan Synchrotron Radiation Research Institute (JASRI/SPring-8), 1-1-1 Kouto, Sayo, Hyogo 679-5198, Japan.
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Mizuno
authors.affiliation: Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan.
authors.forename: Tsunehisa
authors.initials: T
authors.lastname: Kimura
mesh_terms: D000818:Animals; D002645:Chickens; D003460:Crystallization / Q000379:methods; D018360:Crystallography, X-Ray / Q000379:methods*; D003903:Deuterium / Q000737:chemistry; D005782:Gels / Q000737:chemistry*; D060526:Magnetic Fields; D008958:Models, Molecular; D009113:Muramidase / Q000737:chemistry*; D033363:Neutron Diffraction / Q000379:methods*; D011487:Protein Conformation; D014867:Water / Q000737:chemistry
chemical_list: D005782:Gels; D014867:Water; D003903:Deuterium; D009113:Muramidase
keywords: X-ray diffraction; lysozyme; magnetically oriented microcrystal array; neutron diffraction