title: Role of CD34, vascular endothelial growth factor, and p53 in neoangiogenesis as correlated with stage of disease in colorectal carcinoma.
abstract: BACKGROUND
  Angiogenesis is critical for the growth of colorectal carcinoma (CRC). Vascular endothelial growth factor is the most important angiogenic growth factor.
  
  
  METHODS
  Fifty cases of CRC operated at INHS Asvini were studied by using immunohistochemical labelling of the tumours by using CD34, p53, and vascular endothelial growth factor (VEGF).
  
  
  RESULTS
  Out of the 50 cases, 31 (62%) were positive for p53; of which 80.7% showed high expression. Significant staining (> 20% tumour cells showing positivity) was seen in 33 patients (66%), while 34% were negative. Of the 33 positive cases, 57.58% showed high-grade VEGF positivity.
  
  
  CONCLUSIONS
  Vascular endothelial growth factor correlated significantly with the stage and grade of disease. Intra tumours microvessel density as calculated from CD34 expression increased with the stage and grade of the CRC.
authors.affiliation: Associate Professor, Department of Pathology, AFMC, Pune - 40.
authors.forename: Ajay
authors.initials: A
authors.lastname: Malik
authors.affiliation: Ex-Commanding Officer, INHS Kalyani.
authors.forename: R N
authors.initials: RN
authors.lastname: Mishra
authors.affiliation: Commanding Officer, INHS Kalyani.
authors.forename: B
authors.initials: B
authors.lastname: Fanthome
authors.affiliation: Classified Specialist (Pathology), INHS Asvini.
authors.forename: Ramesh
authors.initials: R
authors.lastname: Rao
authors.affiliation: Biostatistician, Department of Community Medicine, AFMC, Pune - 40.
authors.forename: S R
authors.initials: SR
authors.lastname: Patrikar
keywords: angiogenesis; colorectal carcinoma; immunohistochemistry