title: miR-106a-5p Suppresses the Proliferation, Migration, and Invasion of Osteosarcoma Cells by Targeting HMGA2.
abstract: We aim to investigate the effect of miR-106a-5p on the proliferation, migration, and invasion of osteosarcoma (OS) cells by targeting high-mobility group AT-hook 2 (HMGA2). Real-time fluorescent quantitative polymerase chain reaction (RT-qPCR) was used for detecting the expressions of miR-106-5p and HMGA2 in 137 OS and adjacent normal bone tissues. Immunohistochemistry was applied for the HMGA2 protein expression detection. Luciferase reporter gene assay was conducted for verifying whether miR-106-5p targeted HMGA2. MG63 and U2SO cells were respectively divided into five groups: Blank, miR-106a-5p, scramble, HMGA2-siRNA, and miR-106a-5p+HMGA2 groups. RT-qPCR and western blot were applied for detecting the expressions of miR-106a-5p and HMGA2 in five groups. Proliferation rate, cell cycle, invasion, and migration ability of OS cells were detected using methyl thiazolyl-tetrazolium, 5-ethynyl-2'-deoxyuridine (Edu) assay, flow cytometry, and Transwell.  Compared with adjacent normal tissues, OS tissues presented with decreased miR-106a-5p expressions, elevated HMGA2 mRNA, and positive expressions (all p < 0.05). The sensitivity and specificity of miR-106a-5p were 97.8%, 93.43%, and HMGA2 mRNA were 97.8%, 99.27%, separately. miR-106a-5p and HMGA2 expressions were associated with tumor size, Enneking stage, distant metastasis, and lung metastasis. Expressions of HMGA2 in OS cells in miR-106a-5p and HMGA2 siRNA groups were both significantly decreased with the same downregulation level, and the proliferation rates in both groups were obviously slowed down after 48 h (both p < 0.001). Edu positive cells, S phase cells (majority of cells blocked at G0/G1 phase), migratory and invasive cells were obviously decreased (all p < 0.05). Downregulation of miR-106a-5p was found in OS tissues, and upregulation of miR-106a-5p can inhibit the proliferation, migration, and invasion by targeting HMGA2 in OS cells.
authors.affiliation: 1 Department of Traumatic Orthopedics, Changhai Hospital, The Second Military Medical University , Shanghai, People's Republic of China .
authors.forename: Qian-Yun
authors.initials: QY
authors.lastname: He
authors.affiliation: 1 Department of Traumatic Orthopedics, Changhai Hospital, The Second Military Medical University , Shanghai, People's Republic of China .
authors.forename: Guang-Chao
authors.initials: GC
authors.lastname: Wang
authors.affiliation: 1 Department of Traumatic Orthopedics, Changhai Hospital, The Second Military Medical University , Shanghai, People's Republic of China .
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: 1 Department of Traumatic Orthopedics, Changhai Hospital, The Second Military Medical University , Shanghai, People's Republic of China .
authors.forename: Da-Ke
authors.initials: DK
authors.lastname: Tong
authors.affiliation: 1 Department of Traumatic Orthopedics, Changhai Hospital, The Second Military Medical University , Shanghai, People's Republic of China .
authors.forename: Chen
authors.initials: C
authors.lastname: Ding
authors.affiliation: 1 Department of Traumatic Orthopedics, Changhai Hospital, The Second Military Medical University , Shanghai, People's Republic of China .
authors.forename: Kang
authors.initials: K
authors.lastname: Liu
authors.affiliation: 1 Department of Traumatic Orthopedics, Changhai Hospital, The Second Military Medical University , Shanghai, People's Republic of China .
authors.forename: Fang
authors.initials: F
authors.lastname: Ji
authors.affiliation: 2 Department of Orthopedic Surgery, The First Affiliate Hospital of Wenzhou Medical University , Wenzhou, People's Republic of China .
authors.forename: Xiongbai
authors.initials: X
authors.lastname: Zhu
authors.affiliation: 2 Department of Orthopedic Surgery, The First Affiliate Hospital of Wenzhou Medical University , Wenzhou, People's Republic of China .
authors.forename: Shengwu
authors.initials: S
authors.lastname: Yang
mesh_terms: D000293:Adolescent; D001859:Bone Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002460:Cell Line; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D002648:Child; D002675:Child, Preschool; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D025743:HMGA2 Protein / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000556:secondary; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009361:Neoplasm Invasiveness; D010006:Osteoblasts / Q000378:metabolism / Q000473:pathology; D012516:Osteosarcoma / Q000235:genetics* / Q000378:metabolism / Q000556:secondary; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D055815:Young Adult
chemical_list: D025743:HMGA2 Protein; C542541:MIRN106 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering