title: Involvement of the T-box transcription factor Brachyury in early-stage embryonic mouse salivary gland.
abstract: The mouse submandibular gland (SMG) is important organ for embryonic development, and branching morphogenesis is regulated by many molecules containing transcription factors. Real-time reverse transcriptase polymerase chain reaction revealed that the expression of Brachyury increased in the SMG and peaked between E12.5-E13.5, concomitant with the early stage of branching morphogenesis. The expression of Brachyury in SMG rudiments between E12.5-E13.5 was confirmed by western blotting. In addition, fibronectin and Btbd7 (regulated by fibronectin), which are both essential for cleft formation, were expressed strongly during the same period. The Sox2 and Wnt3a, which regulate cell growth, were also expressed strongly during E12.5-E13.5. On the other hand, cleft formation and branching morphogenesis was suppressed by knockdown of Brachyury gene, suggesting that Brachyury plays a central role in regulating cell growth and cleft formation in early-stage embryonic mouse salivary gland development.
authors.affiliation: Section of Oral and Maxillofacial Surgery, Division of Maxillofacial Diagnostic and Surgical Science, Faculty of Dental Science, Kyushu University, Japan. Electronic address: hayakou@dent.kyushu-u.a.cjp.
authors.forename: Kouhei
authors.initials: K
authors.lastname: Hayashi
authors.affiliation: Section of Oral and Maxillofacial Surgery, Division of Maxillofacial Diagnostic and Surgical Science, Faculty of Dental Science, Kyushu University, Japan. Electronic address: ikapon@dent.kyushu-u.ac.jp.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Ikari
authors.affiliation: Section of Oral and Maxillofacial Surgery, Division of Maxillofacial Diagnostic and Surgical Science, Faculty of Dental Science, Kyushu University, Japan. Electronic address: gs5603@dent.kyushu-u.ac.jp.
authors.forename: Goro
authors.initials: G
authors.lastname: Sugiyama
authors.affiliation: Maxillofacial Diagnostic and Surgical Sciences, Department of Oral and Maxillofacial Rehabilitation, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima 890-8520, Japan. Electronic address: sugiura@dent.kyushu-u.ac.jp.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Sugiura
authors.affiliation: Section of Oral and Maxillofacial Surgery, Division of Maxillofacial Diagnostic and Surgical Science, Faculty of Dental Science, Kyushu University, Japan. Electronic address: yuki@dent.kyushu-u.ac.jp.
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Ohyama
authors.affiliation: Section of Oral and Maxillofacial Surgery, Division of Maxillofacial Diagnostic and Surgical Science, Faculty of Dental Science, Kyushu University, Japan. Electronic address: kumamaru@dent.kyushu-u.ac.jp.
authors.forename: Wataru
authors.initials: W
authors.lastname: Kumamaru
authors.affiliation: Section of Oral and Maxillofacial Surgery, Division of Maxillofacial Diagnostic and Surgical Science, Faculty of Dental Science, Kyushu University, Japan. Electronic address: kshira@opal.ocn.ne.jp.
authors.forename: Kanemitsu
authors.initials: K
authors.lastname: Shirasuna
authors.affiliation: Section of Oral and Maxillofacial Surgery, Division of Maxillofacial Diagnostic and Surgical Science, Faculty of Dental Science, Kyushu University, Japan. Electronic address: y-mori@dent.kyushu-u.ac.jp.
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Mori
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D005326:Fetal Proteins / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D051379:Mice; D008813:Mice, Inbred ICR; D009024:Morphogenesis / Q000502:physiology*; D009687:Nuclear Proteins / Q000378:metabolism; D055748:SOXB1 Transcription Factors / Q000378:metabolism*; D012469:Salivary Glands / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D020825:T-Box Domain Proteins / Q000378:metabolism*; D014018:Tissue Distribution; D000098908:Brachyury Protein
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D005326:Fetal Proteins; D005353:Fibronectins; D009687:Nuclear Proteins; D055748:SOXB1 Transcription Factors; D020825:T-Box Domain Proteins; D000098908:Brachyury Protein; C551480:Btbd7 protein, mouse; C527783:Sox2 protein, mouse
keywords: Brachyury; Branching morphogenesis; Cleft formation; Salivary gland