title: [Y-27632 reduces the MMP2 and MMP9 expression in endothelial cell via inhibition of ROCK signal pathway].
abstract: OBJECTIVE
  To explore the effect of ROCK inhibitor Y-27632 on the matrix metalloproteinase 2 and 9 (MMP2 and MMP9) gene expression and activity in tumor necrosis factor α (TNF-α)-treated human umbilical vein endothelial cell (HUVEC).
  
  
  METHODS
  HHUVEC was divided into 3 groups, a control group, a TNF-α group, and a TNF-α plus Y-27632 group. The expressions of vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1), MMP2 and MMP9 were examined by real-time PCR. The MMP2/9 activity was measured by gelatin zymography.
  
  
  RESULTS
  Compared to the control group, the mRNA expressions of ICAM-1, VCAM-1, MMP2 and MMP9 were increased TNF-α-treated cells, which were suppressed by ROCK inhibitor (P<0.01). The MMP2/9 activity was elevated in TNF-α-treated cells, which was reversed by ROCK inhibitor (P<0.05).
  
  
  CONCLUSIONS
  ROCK inhibitor can suppress TNF-α-induced inflammation in endothelial cells through down-regulation of MMP2/9.
authors.affiliation: Department of Cardiology, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Mao
authors.affiliation: Yiyang Medical College, Yiyang Hunan 413000, China.
authors.forename: Zhengxiang
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Department of Cardiology, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Xia
authors.affiliation: Department of Cardiology, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Duan
authors.affiliation: Department of Cardiology, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Yixia
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Cardiology, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Dafeng
authors.initials: D
authors.lastname: Yang
authors.affiliation: Department of Cardiology, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Department of Cardiology, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Cardiology, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Tianlun
authors.initials: T
authors.lastname: Yang
mesh_terms: D000577:Amides; D002478:Cells, Cultured; D015536:Down-Regulation; D042783:Endothelial Cells; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D011725:Pyridines; D015398:Signal Transduction; D014409:Tumor Necrosis Factor-alpha; D014471:Umbilical Veins; D019010:Vascular Cell Adhesion Molecule-1; D054460:rho-Associated Kinases
chemical_list: D000577:Amides; D011725:Pyridines; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; C108830:Y 27632; D054460:rho-Associated Kinases; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9