title: On-Chip Quantitative Measurement of Mechanical Stresses During Cell Migration with Emulsion Droplets.
abstract: The ability of immune cells to migrate within narrow and crowded spaces is a critical feature involved in various physiological processes from immune response to metastasis. Several in-vitro techniques have been developed so far to study the behaviour of migrating cells, the most recent being based on the fabrication of microchannels within which cells move. To address the question of the mechanical stress a cell is able to produce during the encounter of an obstacle while migrating, we developed a hybrid microchip made of parallel PDMS channels in which oil droplets are sparsely distributed and serve as deformable obstacles. We thus show that cells strongly deform droplets while passing them. Then, we show that the microdevice can be used to study the influence of drugs on migration at the population level. Finally, we describe a quantitative analysis method of the droplet deformation that allows measuring in real-time the mechanical stress exerted by a single cell. The method presented herein thus constitutes a powerful analytical tool for cell migration studies under confinement.
authors.affiliation: École Normale Supérieure - PSL Research University, Département de Chimie, 24 rue Lhomond, F-75005 Paris, France.|Sorbonne Universités, UPMC Univ. Paris 06, PASTEUR, F-75005, Paris, France.|CNRS, UMR 8640 PASTEUR, F-75005, Paris, France.
authors.forename: D
authors.initials: D
authors.lastname: Molino
authors.affiliation: École Normale Supérieure - PSL Research University, Département de Chimie, 24 rue Lhomond, F-75005 Paris, France.|Sorbonne Universités, UPMC Univ. Paris 06, PASTEUR, F-75005, Paris, France.|CNRS, UMR 8640 PASTEUR, F-75005, Paris, France.
authors.forename: S
authors.initials: S
authors.lastname: Quignard
authors.affiliation: Laboratoire de Physique Statistique, Ecole Normale Supérieure, Université Pierre et Marie Curie, Université Paris Diderot, Centre National de la Recherche Scientifique UMR8550, 24 rue Lhomond, Paris 75005, France.
authors.forename: C
authors.initials: C
authors.lastname: Gruget
authors.affiliation: Laboratoire de Physique Statistique, Ecole Normale Supérieure, Université Pierre et Marie Curie, Université Paris Diderot, Centre National de la Recherche Scientifique UMR8550, 24 rue Lhomond, Paris 75005, France.
authors.forename: F
authors.initials: F
authors.lastname: Pincet
authors.affiliation: École Normale Supérieure - PSL Research University, Département de Chimie, 24 rue Lhomond, F-75005 Paris, France.|Sorbonne Universités, UPMC Univ. Paris 06, PASTEUR, F-75005, Paris, France.|CNRS, UMR 8640 PASTEUR, F-75005, Paris, France.
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Institut Curie, CNRS UMR 144, 26 rue d'Ulm, 75005, Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Piel
authors.affiliation: École Normale Supérieure - PSL Research University, Département de Chimie, 24 rue Lhomond, F-75005 Paris, France.|Sorbonne Universités, UPMC Univ. Paris 06, PASTEUR, F-75005, Paris, France.|CNRS, UMR 8640 PASTEUR, F-75005, Paris, France.
authors.forename: J
authors.initials: J
authors.lastname: Fattaccioli
mesh_terms: D000577:Amides / Q000494:pharmacology; D002465:Cell Movement / Q000187:drug effects; D004655:Emulsions / Q000737:chemistry*; D018922:HL-60 Cells; D006801:Humans; D056656:Lab-On-A-Chip Devices; D009716:Numerical Analysis, Computer-Assisted; D011725:Pyridines / Q000494:pharmacology; D013314:Stress, Mechanical; D013500:Surface Tension / Q000187:drug effects; D059008:Time-Lapse Imaging
chemical_list: D000577:Amides; D004655:Emulsions; D011725:Pyridines; C108830:Y 27632