title: Cytotoxic effect of fucoidan extracted from Sargassum cinereum on colon cancer cell line HCT-15.
abstract: The present study was aimed to investigate the antioxidant and cytotoxicity activity against HCT-15 of fucoidan from Sargassum cinereum. Purification of fucoidan was done by DEAE cellulose and dialysis. Physicochemical characterization of fucoidan was analysed by calorimetric assay, FT-IR, HPLC and NMR. The extracted fucoidan contains 65.753% of fucose and 3.7±1.54% of sulphate respectively. HPLC results showed that the fucoidan contains the monosaccharide composition such as fucose, galactose, mannose and xylose. Antioxidant effect of fucoidan in Sargassum Cinereum was determined by DPPH. The maximum DPPH activity was found at the concentration of 100μg, where as the crude extract showed the scavenging activity was 63.58±0.56%. Cytotoxicity effect was done by MTT assay. Fucoidan extract caused about 50% of cell death after 24h of incubation with 75±0.9037μg/ml against HCT-15.
authors.affiliation: Department of Marine Microbiology, Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamil Nadu, India. Electronic address: sivasan91@gmail.com.
authors.forename: Sivasankara Narayani
authors.initials: SN
authors.lastname: Somasundaram
authors.affiliation: Department of Marine Microbiology, Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamil Nadu, India. Electronic address: drssaravanan1967@gmail.com.
authors.forename: Saravanan
authors.initials: S
authors.lastname: Shanmugam
authors.affiliation: Department of Marine Microbiology, Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608 502, Tamil Nadu, India. Electronic address: sbrbtc@gmail.com.
authors.forename: Bharathiraja
authors.initials: B
authors.lastname: Subramanian
authors.affiliation: Pathology Discipline, Laboratory based Department, Faculty of Medicine, Universiti Kuala Lumpur, Royal College of Medicine Perak, 30450 Ipoh, Malaysia. Electronic address: jravimicro@gmail.com.
authors.forename: Ravindran
authors.initials: R
authors.lastname: Jaganathan
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000737:chemistry; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000473:pathology*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D009005:Monosaccharides / Q000032:analysis; D010851:Picrates / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D044647:Sargassum / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D009005:Monosaccharides; D010851:Picrates; D011134:Polysaccharides; C007789:fucoidan; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Antioxidant; Colon cancer; Cytotoxic; Fucoidan; Polysaccharide