title: Micron-sized and submicron-sized aerosol deposition in a new ex vivo preclinical model.
abstract: The knowledge of where particles deposit in the respiratory tract is crucial for understanding the health effects associated with inhaled drug particles.
  
  
  
  An ex vivo study was conducted to assess regional deposition patterns (thoracic vs. extrathoracic) of radioactive polydisperse aerosols with different size ranges [0.15 μm-0.5 μm], [0.25 μm-1 μm] and [1 μm-9 μm]. SPECT/CT analyses were performed complementary in order to assess more precisely the regional deposition of aerosols within the pulmonary tract. Experiments were set using an original respiratory tract model composed of a human plastinated head connected to an ex vivo porcine pulmonary tract. The model was ventilated by passive expansion, simulating pleural depressions. Aerosol was administered during nasal breathing.
  
  
  
  Planar scintigraphies allowed to calculate the deposited aerosol fractions for particles in the three size ranges from sub-micron to micron The deposited fractions obtained, for thoracic vs. extra-thoracic regions respectively, were 89 ± 4 % vs. 11 ± 4 % for [0.15 μm-0.5 μm], 78 ± 5 % vs. 22 ± 5 % for [0.25 μm-1 μm] and 35 ± 11 % vs.65 ± 11 % for [1 μm-9 μm].
  
  
  
  Results obtained with this new ex vivo respiratory tract model are in good agreement with the in vivo data obtained in studies with baboons and humans.
authors.affiliation: INSERM, U1059, SAINBIOSE, Saint-Etienne, F-42023, France.|Université de Lyon, Saint-Etienne, F-42023, France.|CHU Saint-Etienne, Saint-Etienne, F-42055, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Perinel
authors.affiliation: INSERM, U1059, SAINBIOSE, Saint-Etienne, F-42023, France. leclerc@emse.fr.|Université de Lyon, Saint-Etienne, F-42023, France. leclerc@emse.fr.|Ecole Nationale Supérieure des Mines de Saint-Etienne, CIS-EMSE, SAINBIOSE, F-42023, Saint Etienne, France. leclerc@emse.fr.
authors.forename: Lara
authors.initials: L
authors.lastname: Leclerc
authors.affiliation: INSERM, U1059, SAINBIOSE, Saint-Etienne, F-42023, France.|Université de Lyon, Saint-Etienne, F-42023, France.|CHU Saint-Etienne, Saint-Etienne, F-42055, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Prévôt
authors.affiliation: Université de Lyon, Saint-Etienne, F-42023, France.|CHU Saint-Etienne, Saint-Etienne, F-42055, France.
authors.forename: Agathe
authors.initials: A
authors.lastname: Deville
authors.affiliation: INSERM, U1059, SAINBIOSE, Saint-Etienne, F-42023, France.|Université de Lyon, Saint-Etienne, F-42023, France.|CHU Saint-Etienne, Saint-Etienne, F-42055, France.
authors.forename: Michèle
authors.initials: M
authors.lastname: Cottier
authors.affiliation: INSERM, U1059, SAINBIOSE, Saint-Etienne, F-42023, France.|Centre Hospitalier Emile Roux, F-43012, Le Puy en Velay, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Durand
authors.affiliation: INSERM, U1059, SAINBIOSE, Saint-Etienne, F-42023, France.|Université de Lyon, Saint-Etienne, F-42023, France.|CHU Saint-Etienne, Saint-Etienne, F-42055, France.
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Vergnon
authors.affiliation: INSERM, U1059, SAINBIOSE, Saint-Etienne, F-42023, France.|Université de Lyon, Saint-Etienne, F-42023, France.|Ecole Nationale Supérieure des Mines de Saint-Etienne, CIS-EMSE, SAINBIOSE, F-42023, Saint Etienne, France.
authors.forename: Jérémie
authors.initials: J
authors.lastname: Pourchez
mesh_terms: D000280:Administration, Inhalation; D000336:Aerosols; D000818:Animals; D006257:Head / Q000033:anatomy & histology*; D006801:Humans; D008953:Models, Anatomic; D010316:Particle Size; D019275:Radiopharmaceuticals / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism*; D012121:Respiration, Artificial; D012137:Respiratory System / Q000033:anatomy & histology* / Q000000981:diagnostic imaging / Q000378:metabolism*; D000072098:Single Photon Emission Computed Tomography Computed Tomography; D034421:Sus scrofa; D016284:Technetium Tc 99m Pentetate / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D000336:Aerosols; D019275:Radiopharmaceuticals; D016284:Technetium Tc 99m Pentetate
keywords: Aerosol deposition; Aerosoltherapy; SPECT/CT imaging