title: Mycobacterium lutetiense sp. nov., Mycobacterium montmartrense sp. nov. and Mycobacterium arcueilense sp. nov., members of a novel group of non-pigmented rapidly growing mycobacteria recovered from a water distribution system.
abstract: From our recent survey of non-pigmented rapidly growing mycobacteria in the Parisian water system, three groups of isolates (taxons 1-3) corresponding to possible novel species were selected for taxonomic study. The three taxa each formed creamy white, rough colonies, had an optimal growth temperature of 30 °C, hydrolyzed Tween 80, were catalase-positive at 22 °C and expressed arylsulfatase activity. All three were susceptible to amikacin, ciprofloxacin and tigecycline. The three taxa produced specific sets of mycolic acids, including one family that has never previously been described, as determined by thin layer chromatography and nuclear magnetic resonance. The partial rpoB sequences (723 bp) showed 4-6 % divergence from each other and more than 5 % differences from the most similar species. Partial 16S rRNA gene sequences showed 99 % identity within each species. The most similar sequences for 16S rRNA genes (98-99 % identity over 1444-1461 bp) were found in the Mycobacterium fortuitum group, Mycobacterium septicum and Mycobacterium farcinogenes. The three taxa formed a new clade (bootstrap value, 99 %) on trees reconstructed from concatenated partial 16S rRNA, hsp65 and rpoB sequences. The above results led us to propose three novel species for the three groups of isolates, namely Mycobacterium lutetiense sp. nov. [type strain 071T=ParisRGMnew_1T (CIP 110656T=DSM 46713T)], Mycobacterium montmartrense sp. nov. [type strain 196T=ParisRGMnew_2T (CIP 110655T=DSM 46714T)] and Mycobacteriu marcueilense sp. nov. [type strain of 269T=ParisRGMnew_3T (CIP 110654T=DSM 46715T)].
authors.affiliation: INSERM U1173, UFR Simone Veil, Versailles-Saint-Quentin University, 78180 Saint-Quentin en Yvelines, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Konjek
authors.affiliation: INSERM U1173, UFR Simone Veil, Versailles-Saint-Quentin University, 78180 Saint-Quentin en Yvelines, France.
authors.forename: Sabiha
authors.initials: S
authors.lastname: Souded
authors.affiliation: Unité de Glycobiologie Structurale et Fonctionnelle, CNRS UMR 8576, Université Lille1, Villeneuve d'Ascq, France.
authors.forename: Yann
authors.initials: Y
authors.lastname: Guerardel
authors.affiliation: Unité de Glycobiologie Structurale et Fonctionnelle, CNRS UMR 8576, Université Lille1, Villeneuve d'Ascq, France.
authors.forename: Xavier
authors.initials: X
authors.lastname: Trivelli
authors.affiliation: Centre National de la Recherche Scientifique FRE 3689, Centre d'études d'agents Pathogènes et Biotechnologies pour la Santé, Université de Montpellier, 1919 route de Mende, 34293, Montpellier, France.
authors.forename: Audrey
authors.initials: A
authors.lastname: Bernut
authors.affiliation: Centre National de la Recherche Scientifique FRE 3689, Centre d'études d'agents Pathogènes et Biotechnologies pour la Santé, Université de Montpellier, 1919 route de Mende, 34293, Montpellier, France.|INSERM, CPBS, 34293 Montpellier, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Kremer
authors.affiliation: Eau de Paris, Direction de la recherche, du développement et de la qualité de l'eau, Paris, France.
authors.forename: Benedicte
authors.initials: B
authors.lastname: Welte
authors.affiliation: Eau de Paris, Direction de la recherche, du développement et de la qualité de l'eau, Paris, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Joyeux
authors.affiliation: Laboratoire d'Hygiène de la Ville de Paris, Paris, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Dubrou
authors.affiliation: Laboratoire de Bactériologie, Ecole Nationale Vétérinaire, Toulouse, France.
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Euzeby
authors.affiliation: INSERM U1173, UFR Simone Veil, Versailles-Saint-Quentin University, 78180 Saint-Quentin en Yvelines, France.|Hôpitaux universitaires Paris Ile-de-France Ouest, site Ambroise Paré, Service de Microbiologie, Assistance Publique - Hôpitaux de Paris, Boulogne-Billancourt, France.
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Gaillard
authors.affiliation: INSERM U1173, UFR Simone Veil, Versailles-Saint-Quentin University, 78180 Saint-Quentin en Yvelines, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Sapriel
authors.affiliation: Hôpitaux universitaires Paris Ile-de-France Ouest, site Ambroise Paré, Service de Microbiologie, Assistance Publique - Hôpitaux de Paris, Boulogne-Billancourt, France.|INSERM U1173, UFR Simone Veil, Versailles-Saint-Quentin University, 78180 Saint-Quentin en Yvelines, France.
authors.forename: Beate
authors.initials: B
authors.lastname: Heym
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009171:Mycolic Acids / Q000378:metabolism; D010297:Paris; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014871:Water Microbiology; D014881:Water Supply
chemical_list: D004269:DNA, Bacterial; D009171:Mycolic Acids; D012336:RNA, Ribosomal, 16S