title: Optical biosensors.
abstract: Optical biosensors represent the most common type of biosensor. Here we provide a brief classification, a description of underlying principles of operation and their bioanalytical applications. The main focus is placed on the most widely used optical biosensors which are surface plasmon resonance (SPR)-based biosensors including SPR imaging and localized SPR. In addition, other optical biosensor systems are described, such as evanescent wave fluorescence and bioluminescent optical fibre biosensors, as well as interferometric, ellipsometric and reflectometric interference spectroscopy and surface-enhanced Raman scattering biosensors. The optical biosensors discussed here allow the sensitive and selective detection of a wide range of analytes including viruses, toxins, drugs, antibodies, tumour biomarkers and tumour cells.
authors.affiliation: Institute of Chemistry, Slovak Academy of Sciences, Dubravska cesta 9, 845 38 Bratislava, Slovakia.
authors.forename: Pavel
authors.initials: P
authors.lastname: Damborský
authors.affiliation: Institute of Biotechnology and Food Science, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinskeho 9, 81237 Bratislava, Slovakia.
authors.forename: Juraj
authors.initials: J
authors.lastname: Švitel
authors.affiliation: Institute of Chemistry, Slovak Academy of Sciences, Dubravska cesta 9, 845 38 Bratislava, Slovakia chemjkat@savba.sk.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Katrlík
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D005336:Fiber Optic Technology / Q000379:methods*; D008164:Luminescent Proteins / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects; D061848:Optical Imaging / Q000379:methods*; D020349:Surface Plasmon Resonance / Q000379:methods
chemical_list: D008164:Luminescent Proteins
keywords: biosensor; optical biosensing; surface plasmon resonance