title: Anti-ulcerogenic effect of cavidine against ethanol-induced acute gastric ulcer in mice and possible underlying mechanism.
abstract: Cavidine, a major alkaloid compound isolated from Corydalis impatiens, has various pharmacological effects but its effect on gastric ulcer has not been previously explored. The current study aimed to investigate the possible anti-ulcerogenic potential of cavidine in the model of ethanol-induced gastric ulcer. Mice received cavidine (1, 5 or 10mg/kg, ig), cimetidine (CMD, 100mg/kg, ig) or vehicle at 12h and 1h before absolute ethanol administration (0.5mL/100g), and animals were euthanized 3h after ethanol ingestion. Gross and histological gastric lesions, biochemical, immunological and Western blot parameters were taken into consideration. The results showed that ethanol administration produced apparent mucosal injuries with morphological and histological damage, whereas cavidine pre-treatment reduced the gastric injuries. Cavidine pre-treatment also ameliorated the contents of malonaldehyde (MDA) and myeloperoxidase (MPO) activity, and increased the mucosa levels of glutathione (GSH), superoxide dismutase (SOD) and prostaglandin E2 (PGE2), relative to the model group. Also cavidine was able to decrease the levels of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α), inhibit the up-regulation of cyclo-oxygenase-2 (COX-2) expression and activation of Nuclear factor-kappa B (NF-κB) pathway. Taken together, these results indicated that cavidine exerts a gastroprotective effect against gastric ulceration, and the underlying mechanism might be associated with the stimulation of PGE2, reduction of oxidative stress, suppression of NF-κB expression and subsequent reduced COX-2 and pro-inflammatory cytokines.
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an, 710061, PR China. Electronic address: liwf@mail.xjtu.edu.cn.
authors.forename: Weifeng
authors.initials: W
authors.lastname: Li
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an, 710061, PR China.
authors.forename: Xiumei
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an, 710061, PR China.
authors.forename: Hailin
authors.initials: H
authors.lastname: Zhang
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an, 710061, PR China.
authors.forename: Zehong
authors.initials: Z
authors.lastname: He
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an, 710061, PR China.
authors.forename: Wenbing
authors.initials: W
authors.lastname: Zhi
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an, 710061, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an, 710061, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Xi'an Jiaotong University, Xi'an, 710061, PR China. Electronic address: niuxf@mail.xjtu.edu.cn.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Niu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D000975:Antioxidants / Q000008:administration & dosage / Q000627:therapeutic use*; D001600:Berberine Alkaloids / Q000627:therapeutic use*; D031311:Corydalis / Q000276:immunology*; D051546:Cyclooxygenase 2 / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D000431:Ethanol; D005753:Gastric Mucosa / Q000187:drug effects* / Q000276:immunology; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D016328:NF-kappa B / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy*; D000072105:Superoxide Dismutase-1 / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D001600:Berberine Alkaloids; D015850:Interleukin-6; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; C011188:cavidine; D000431:Ethanol; D009195:Peroxidase; D051546:Cyclooxygenase 2; C000606291:Sod1 protein, mouse; D000072105:Superoxide Dismutase-1; D015232:Dinoprostone
keywords: Cavidine; Ethanol; Gastric ulcer; Inflammation; Oxidative stress