title: IFN-α, IFN-β, and IFN-γ Have Different Effect on the Production of Proinflammatory Factors Deposited in Weibel-Palade Bodies of Endothelial Cells Infected with Herpes Simplex Virus Type 1.
abstract: We demonstrated similarities and differences in the effects of IFN-α and IFN-β compared to IFN-γ on the production of factors deposited in the Weibel-Palade bodies in cultures of endothelial cells (intact and infected with herpes simplex virus 1). IFN-α and IFN-β reduced the content of von Willebrand factor, endothelin-1, and soluble P-selectin and increased IL-8 concentration in the culture medium of human umbilical vein endothelial cells. IFN-γ reduced the content of all studied factors in the endothelial cell culture medium. Possible mechanisms of these effects are discussed.
authors.affiliation: N. F. Gamalea Institute of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia. scheglovitova@rambler.ru.
authors.forename: O N
authors.initials: ON
authors.lastname: Shcheglovitova
authors.affiliation: N. F. Gamalea Institute of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Boldyreva
authors.affiliation: N. F. Gamalea Institute of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: N N
authors.initials: NN
authors.lastname: Sklyankina
authors.affiliation: N. F. Gamalea Institute of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Babayants
authors.affiliation: N. F. Gamalea Institute of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia.
authors.forename: I S
authors.initials: IS
authors.lastname: Frolova
mesh_terms: D002478:Cells, Cultured; D019332:Endothelin-1 / Q000096:biosynthesis; D006561:Herpes Simplex / Q000276:immunology* / Q000378:metabolism; D018259:Herpesvirus 1, Human / Q000276:immunology*; D061307:Human Umbilical Vein Endothelial Cells / Q000276:immunology / Q000378:metabolism* / Q000821:virology; D006801:Humans; D007372:Interferons / Q000502:physiology*; D016209:Interleukin-8 / Q000096:biosynthesis; D019007:P-Selectin / Q000096:biosynthesis; D020302:Weibel-Palade Bodies / Q000378:metabolism*; D014841:von Willebrand Factor / Q000096:biosynthesis
chemical_list: D019332:Endothelin-1; D016209:Interleukin-8; D019007:P-Selectin; D014841:von Willebrand Factor; D007372:Interferons
keywords: HSV-1; IL-8; Weibel–Palade bodies; soluble P-selectin; vascular endothelium; von Willebrand factor