title: Analysis of Nucleosome Sliding by ATP-Dependent Chromatin Remodeling Enzymes.
abstract: ATP-dependent chromatin remodeling complexes carry out diverse transformations of chromatin. Understanding their mechanisms requires assays that can monitor the kinetics or chromatin remodeling. In this chapter, we describe complimentary native gel-based and FRET-based methods for assaying the kinetics of ATP-driven nucleosome sliding. These methods can be readily adapted to investigate other types of nucleosomal transformations carried out by chromatin remodeling ATPases.
authors.affiliation: University of California, San Francisco, CA, United States.
authors.forename: C Y
authors.initials: CY
authors.lastname: Zhou
authors.affiliation: University of California, San Francisco, CA, United States. Electronic address: geeta.narlikar@ucsf.edu.
authors.forename: G J
authors.initials: GJ
authors.lastname: Narlikar
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D042002:Chromatin Assembly and Disassembly; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D007700:Kinetics; D009707:Nucleosomes / Q000378:metabolism*; D014981:Xenopus
chemical_list: D009707:Nucleosomes; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases
keywords: Chromatin remodeling; Enzyme kinetics; Fluorescence resonance energy transfer; Nucleosome labeling