title: Tuning the architectural integrity of high-performance magneto-fluorescent core-shell nanoassemblies in cancer cells.
abstract: High-density nanoarchitectures, endowed with simultaneous fluorescence and contrast properties for MRI and TEM imaging, have been obtained using a simple self-assembling strategy based on supramolecular interactions between non-doped fluorescent organic nanoparticles (FON) and superparamagnetic nanoparticles. In this way, a high-payload core-shell structure FON@mag has been obtained, protecting the hydrophobic fluorophores from the surroundings as well as from emission quenching by the shell of magnetic nanoparticles. Compared to isolated nanoparticles, maghemite nanoparticles self-assembled as an external shell create large inhomogeneous magnetic field, which causes enhanced transverse relaxivity and exacerbated MRI contrast. The magnetic load of the resulting nanoassemblies is evaluated using magnetic sedimentation and more originally electrospray mass spectrometry. The role of the stabilizing agents (citrate versus polyacrylate anions) revealed to be crucial regarding the cohesion of the resulting high-performance magneto-fluorescent nanoassemblies, which questions their use after cell internalization as nanocarriers or imaging agents for reliable correlative light and electron microcopy.
authors.affiliation: CEISAM-UMR CNRS 6230, Université de Nantes, 2 rue de la Houssinière, 44322 Nantes, France.
authors.forename: Adrien
authors.initials: A
authors.lastname: Faucon
authors.affiliation: UFIP-UMR CNRS 6204, Université de Nantes, 2 rue de la Houssinière, 44322 Nantes, France.
authors.forename: Houda
authors.initials: H
authors.lastname: Benhelli-Mokrani
authors.affiliation: UFIP-UMR CNRS 6204, Université de Nantes, 2 rue de la Houssinière, 44322 Nantes, France.
authors.forename: Fabrice
authors.initials: F
authors.lastname: Fleury
authors.affiliation: Pan Ther-UMR 703, INRA-ONIRIS, Atlanpole-Chanterie, 44307 Nantes, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Dubreil
authors.affiliation: INSERM UMS 016-UMS CNRS 3556, 8 quai Moncousu, 44007 Nantes, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Hulin
authors.affiliation: INSERM UMS 016-UMS CNRS 3556, 8 quai Moncousu, 44007 Nantes, France.
authors.forename: Steven
authors.initials: S
authors.lastname: Nedellec
authors.affiliation: Institut Lumière Matière-UMR CNRS 5306, Université de Lyon, 69622 Villeurbanne cedex, France.
authors.forename: Tristan
authors.initials: T
authors.lastname: Doussineau
authors.affiliation: Institut Lumière Matière-UMR CNRS 5306, Université de Lyon, 69622 Villeurbanne cedex, France.
authors.forename: Rodolphe
authors.initials: R
authors.lastname: Antoine
authors.affiliation: Department of Physics, Università di Pavia, via Bassi, 27100 Pavia, Italy.
authors.forename: Tomas
authors.initials: T
authors.lastname: Orlando
authors.affiliation: Department of Physics, Università di Pavia, via Bassi, 27100 Pavia, Italy; Department of Physics, Università degli Studi di Milano and INSTM, via Celoria 16, 20133 Milano, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Lascialfari
authors.affiliation: Sorbonne Universités, UPMC Univ. Paris 06, CNRS, Laboratoire PHENIX, 4 place Jussieu, 75005 Paris, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Fresnais
authors.affiliation: CEISAM-UMR CNRS 6230, Université de Nantes, 2 rue de la Houssinière, 44322 Nantes, France.
authors.forename: Lénaïc
authors.initials: L
authors.lastname: Lartigue
authors.affiliation: CEISAM-UMR CNRS 6230, Université de Nantes, 2 rue de la Houssinière, 44322 Nantes, France. Electronic address: elena.ishow@univ-nantes.fr.
authors.forename: Eléna
authors.initials: E
authors.lastname: Ishow
mesh_terms: D003287:Contrast Media / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D015394:Molecular Structure; D009369:Neoplasms / Q000473:pathology*; D010316:Particle Size; D013499:Surface Properties; D014407:Tumor Cells, Cultured
chemical_list: D003287:Contrast Media; D005456:Fluorescent Dyes; D058185:Magnetite Nanoparticles
keywords: Bioimaging; Core-shell structure; Fluorescent organic nanoparticles; Magnetism; Magneto-fluorescent nanoassemblies; Supraparticles