title: Reidentification of Facultatively Alkaliphilic Bacillus sp. C-125 to Bacillus halodurans.
abstract: Alkaliphilic Bacillus sp. C-125 was taxonomically characterized by physiological and biochemical characteristics, 16S rDNA sequence similarity, and DNA-DNA hybridization analyses. Phylogenetic analysis of strain C-125 based on comparison of 16S rDNA sequences showed that this strain is closely related to Bacillus halodurans. DNA-DNA hybridization analysis was done comparing C-125 and related Bacillus reference strains. The highest level of DNA-DNA relatedness (86%) was found between strain C-125 and B. halodurans. Our findings demonstrate that strain C-125 is a member of the species B. halodurans.
authors.affiliation: a Deep-sea Microorganisms Research Group, Japan Marine Science and Technology Center.
authors.forename: H
authors.initials: H
authors.lastname: Takami
authors.affiliation: a Deep-sea Microorganisms Research Group, Japan Marine Science and Technology Center.
authors.forename: K
authors.initials: K
authors.lastname: Horikoshi
keywords: DNA-DNA hybridization; alkaliphilic Bacillus halodurans C-125; identification; phylogenetic tree