title: Rapid detection of bovine adipose tissue using lateral flow strip assay.
abstract: Currently no rapid immunoassays are developed to identify the species content of fat tissue in mixtures. We report a simple protocol enabling the effective detection of bovine fat in highly processed materials using a lateral flow (LF) immunoassay which targets a ruminant-specific muscle protein. A portion (50 gm) of muscle-free fat samples was rendered to separate the molten fat from the proteinaceous residue, then soluble proteins were extracted from the solid residue with 0.5 mol/L NaCl for the LF analysis. The assay could detect 2% bovine fat-in-pork fat, 1% bovine fat-in-porcine meat-and-bone meal, and 0.5% bovine fat-in-soy meal mixtures. Rendered bovine fat could be detected up to 213°C. These results demonstrate that low levels of bovine fat tissue can be detected in processed materials using an immunoassay based on the presence of the muscle protein which serves as a species marker in the fat tissue.
authors.affiliation: Department of Nutrition Food and Exercise Sciences Florida State University Tallahassee Florida 32306.
authors.forename: Yun-Hwa P
authors.initials: YH
authors.lastname: Hsieh
authors.affiliation: Flat 12 Hampton Court, Batavia Road London SE14 6AQ United Kingdom.
authors.forename: Kamil
authors.initials: K
authors.lastname: Gajewski
keywords: Adulteration; immunoassay; lipids; proteins