title: Probiotic activity of Enterococcus faecalis CECT7121: effects on mucosal immunity and intestinal epithelial cells.
abstract: OBJECTIVE
  To analyse the effect of Enterococcus faecalis CECT7121 on intestinal epithelial cells (IECs) and its effects on the mucosal immune response.
  
  
  RESULTS
  Enterococcus faecalis CECT7121 showed a high adhesion capacity to completely and heterogeneously differentiated human intestinal epithelial cell line (Caco-2 cells). In addition, the contact of this bacterium with Caco-2 cells did not induce inflammatory chemokines (IL-8 and CCL-20). The presence of IgA(+) and IL-6(+) cells in the small intestine, as well as the production of inflammatory cytokines (TNFα, IL-6 and IL-12) in the gut, was determined after intragastric inoculation of Ent. faecalis CECT7121 in BALB/c mice. The administration of Ent. faecalis CECT7121 increased the number of IgA(+) cells in the intestinal lamina propria without modifying the percentage of IL-6(+) cells. No differences were observed in the cytokines measured in the intestinal extracts between probiotic-treated and control mice.
  
  
  CONCLUSIONS
  Enterococcus faecalis CECT7121 stimulates local mucosal immunity and adheres to IECs without inducing inflammatory signals.
  
  
  CONCLUSIONS
  Our results indicate that, apart from its already reported systemic immune activity, Ent. faecalis CECT7121 has a modulatory effect at a local level.
authors.affiliation: Instituto de Estudios de la Inmunidad Humoral "Prof. Dr. Ricardo A. Margni" (IDEHU CONICET-UBA), Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Cátedra de Inmunología, Junín 956, 4°, C.A.B.A., Argentina. mcastro@ffyb.uba.ar.
authors.forename: M S
authors.initials: MS
authors.lastname: Castro
authors.affiliation: Instituto de Estudios de la Inmunidad Humoral "Prof. Dr. Ricardo A. Margni" (IDEHU CONICET-UBA), Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Cátedra de Inmunología, Junín 956, 4°, C.A.B.A., Argentina.
authors.forename: M A
authors.initials: MA
authors.lastname: Molina
authors.affiliation: Instituto de Estudios de la Inmunidad Humoral "Prof. Dr. Ricardo A. Margni" (IDEHU CONICET-UBA), Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Cátedra de Inmunología, Junín 956, 4°, C.A.B.A., Argentina.
authors.forename: M B
authors.initials: MB
authors.lastname: Azpiroz
authors.affiliation: Instituto de Estudios de la Inmunidad Humoral "Prof. Dr. Ricardo A. Margni" (IDEHU CONICET-UBA), Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Cátedra de Inmunología, Junín 956, 4°, C.A.B.A., Argentina.
authors.forename: A M
authors.initials: AM
authors.lastname: Díaz
authors.affiliation: Universidad de Buenos Aires, Facultad de Medicina, Departamento de Histología, Paraguay 2155, 3°, C.A.B.A., Argentina.
authors.forename: R
authors.initials: R
authors.lastname: Ponzio
authors.affiliation: Cátedra de Microbiología y Parasitología (CUDEMyP), Facultad de Ciencias Médicas, Universidad Nacional de La Plata, Calle 60 y 120, La Plata, Argentina.
authors.forename: M D
authors.initials: MD
authors.lastname: Sparo
authors.affiliation: Instituto de Estudios de la Inmunidad Humoral "Prof. Dr. Ricardo A. Margni" (IDEHU CONICET-UBA), Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Cátedra de Inmunología, Junín 956, 4°, C.A.B.A., Argentina.
authors.forename: M A
authors.initials: MA
authors.lastname: Manghi
authors.affiliation: Instituto de Estudios de la Inmunidad Humoral "Prof. Dr. Ricardo A. Margni" (IDEHU CONICET-UBA), Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Cátedra de Inmunología, Junín 956, 4°, C.A.B.A., Argentina.
authors.forename: A M
authors.initials: AM
authors.lastname: Canellada
mesh_terms: D000818:Animals; D018938:Caco-2 Cells; D016207:Cytokines / Q000096:biosynthesis; D013293:Enterococcus faecalis / Q000502:physiology*; D004847:Epithelial Cells / Q000187:drug effects / Q000276:immunology* / Q000382:microbiology; D006801:Humans; D018928:Immunity, Mucosal / Q000187:drug effects*; D015850:Interleukin-6 / Q000276:immunology; D016209:Interleukin-8 / Q000276:immunology; D007413:Intestinal Mucosa / Q000187:drug effects / Q000276:immunology* / Q000382:microbiology; D007421:Intestine, Small / Q000276:immunology / Q000382:microbiology; D007422:Intestines / Q000187:drug effects / Q000276:immunology* / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D019936:Probiotics / Q000008:administration & dosage*; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D016207:Cytokines; D015850:Interleukin-6; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha
keywords: Enterococcus faecalis CECT7121; adhesion to intestinal epithelial cells; immunomodulation; mucosal immune response; probiotic activity