title: Preparation, characterization and antiglycation activities of the novel polysaccharides from Boletus snicus.
abstract: Boletus snicus (BS) is one of the commercially important mushroom species. Two polysaccharides (BSP-1b and BSP-2b) were extracted and purified from the body of BS by DEAE-cellulose and Sephadex G-100 column chromatography. The average of molecular weight of BSP-1b and BSP-2b were 59.21kDa and 128.74kDa. BSP-1b is a heteropolysaccharide with a large number of glucose and a small amount of mannose, glucosamine hydrochloride and arabinose. The monosaccharide compositions of BSP-2b contain mannose, glucuronic acid, glucosamine hydrochloride, glucose, galactose, arabinose with the molar ratio of 10.70:6.95:12.05:12.57:1.83:1.00. The FTIR spectra and NMR analysis demonstrated that BSP-1b and BSP-2b existed pyranose ring structure and BSP-2b had high content of uronic acid. The antiglycation activities of BSP-1b and BSP-2b were investigated. The results showed BSP-1b and BSP-2b had high inhibitory effects on glycation and exhibited dose-dependent responses. BSP-2b showed stronger antiglycation activity than BSP-1b. This study indicated that the BSP-2b could effectively inhibit the formation of advanced glycation end-products.
authors.affiliation: Yunnan Institute of Food Safety, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.
authors.forename: Sun
authors.initials: S
authors.lastname: Liping
authors.affiliation: Yunnan Institute of Food Safety, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.
authors.forename: Su
authors.initials: S
authors.lastname: Xuejiao
authors.affiliation: Yunnan Institute of Food Safety, Kunming University of Science and Technology, Kunming, Yunnan 650500, China. Electronic address: kmylzhuang@163.com.
authors.forename: Zhuang
authors.initials: Z
authors.lastname: Yongliang
mesh_terms: D001487:Basidiomycota / Q000737:chemistry*; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D017127:Glycation End Products, Advanced; D006031:Glycosylation / Q000187:drug effects; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D009580:Nitroblue Tetrazolium; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D066244:Proton Magnetic Resonance Spectroscopy; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D017127:Glycation End Products, Advanced; D009005:Monosaccharides; D011134:Polysaccharides; D009580:Nitroblue Tetrazolium
keywords: Antiglycation activities; Chemical characterization; Polysaccharides