title: New High-Performance Liquid Chromatography Coupled Mass Spectrometry Method for the Detection of Lobster and Shrimp Allergens in Food Samples via Multiple Reaction Monitoring and Multiple Reaction Monitoring Cubed.
abstract: Crustacean shellfish allergy ranks among the most frequent and severe food allergies for adults, demanding rugged and sensitive analytical routine methods. The objective of this study was therefore to develop a mass spectrometric approach for the detection of contamination with shrimp and lobster, two economically important types of crustaceans, in complex food matrices. Following a biomarker approach, we identified proteotypic peptides and developed a multiple reaction monitoring (MRM) method allowing for the identification and differentiation of shrimp and lobster in the food matrix at concentrations down to 0.1%. To further enhance sensitivity, we employed the MRM-cubed (MRM(3)) mode, which allowed us to detect crustaceans down to concentrations of 25 μg/g (crustacean/food, 0.0025%). We hereby present the first mass spectrometric method for the detection of shrimp and lobster in food matrices.
authors.affiliation: Institute of Food Chemistry, Westfälische Wilhelms-Universität Münster , Corrensstraße 45, 48149 Münster, Germany.
authors.forename: Robin
authors.initials: R
authors.lastname: Korte
authors.affiliation: Phylogene , 62 Rn 113, 30620 Bernis, France.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Monneuse
authors.affiliation: Phylogene , 62 Rn 113, 30620 Bernis, France.
authors.forename: Elodie
authors.initials: E
authors.lastname: Gemrot
authors.affiliation: Phylogene , 62 Rn 113, 30620 Bernis, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Metton
authors.affiliation: Institute of Food Chemistry, Westfälische Wilhelms-Universität Münster , Corrensstraße 45, 48149 Münster, Germany.
authors.forename: Hans-Ulrich
authors.initials: HU
authors.lastname: Humpf
authors.affiliation: Institute of Food Chemistry, Westfälische Wilhelms-Universität Münster , Corrensstraße 45, 48149 Münster, Germany.|Analytical Food Chemistry, University of Stuttgart , Allmandring 5b, 70563 Stuttgart, Germany.
authors.forename: Jens
authors.initials: J
authors.lastname: Brockmeyer
mesh_terms: D000485:Allergens / Q000737:chemistry* / Q000276:immunology; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D033364:Decapoda / Q000737:chemistry / Q000276:immunology*; D013058:Mass Spectrometry / Q000379:methods*; D008121:Nephropidae / Q000737:chemistry / Q000276:immunology*; D012758:Shellfish / Q000032:analysis*
chemical_list: D000485:Allergens
keywords: MRM cubed; crustacean allergy; food allergens; marker peptides; mass spectrometry