title: Noggin Along with a Self-Assembling Peptide Nanofiber Containing Long Motif of Laminin Induces Tyrosine Hydroxylase Gene Expression.
abstract: Tyrosine hydroxylase (TH), a rate-limiting step in catecholamine synthesis in which its activity influences Alzheimer disease, Parkinson disease, and IQ of schizophrenia patients, has been studied for a long time. In the meantime, the present investigation assessed the effect of noggin and type of self-assembling nanofibers in TH gene over-expression by neuron-like cells derived from human endometrial-derived stromal cells (hEnSCs). Neuroblastoma cells and hEnSCs encapsulated into nanofibers including Matrigel, (RADA)4, laminin, and BMHP-1 motif bounded to (RADA)4and their cell viability were studied for 48 h and 18 days in basal and neurogenic media, respectively, in noggin-rich media. Then, expression of neural genes and proteins has been investigated by immunocytochemistry (ICC) and real-time PCR methods, respectively. The results indicated that neuroblastoma cell and hEnSC viability is in good agreement with the level of Bcl2 and β-tubulin III gene expression; however, -BMHP-1 and -laminin nanofibers exhibited significantly higher cell viability eventually through Wnt/β-catenin signaling pathway as compared to others, respectively. The gene expression analysis of nanofibers showed that none of them induced gamma-aminobutyric acid (GABA) gene expression while glial fibrillary acidic protein (GFAP) gene just over-expressed in cells encapsulated into Matrigel with a low level of Bcl2 gene expression. However, the TH gene just had been over-expressed in cells encapsulated into -laminin nanofiber and 2D cell culture. In the absence of noggin with -laminin nanofibers, TH gene expression was suppressed. It might be concluded that although noggin through anti-BMP pathways resulted in GFAP decrement and TH gene increment, the type of scaffold that defined the final fate of cells and -laminin accompaniment might be useful for the recovery of Alzheimer and Parkinson disease patients.
authors.affiliation: Drug Nanocarriers Research Core, Razi Drug Research Center, Iran University of Medical Sciences, Tehran, Iran. tavakol.sh@iums.ac.ir.|Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran. tavakol.sh@iums.ac.ir.|Advance Sciences and Technologies Association, Student's Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran. tavakol.sh@iums.ac.ir.
authors.forename: Shima
authors.initials: S
authors.lastname: Tavakol
authors.affiliation: Shefa Neuroscience Research Center, Khatam al-Anbia Hospital, Tehran, Iran.
authors.forename: Sayed Mostafa Modaress
authors.initials: SMM
authors.lastname: Musavi
authors.affiliation: School of Medicine, Kashan University of Medical Sciences, Isfahan, Iran.
authors.forename: Behnaz
authors.initials: B
authors.lastname: Tavakol
authors.affiliation: Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
authors.forename: Elham
authors.initials: E
authors.lastname: Hoveizi
authors.affiliation: Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.|Brain and Spinal Injury Research Center, Imam Hospital, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Jafar
authors.initials: J
authors.lastname: Ai
authors.affiliation: Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.|Department of Toxicology and Pharmacology, School of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran.|Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Seyed Mahdi
authors.initials: SM
authors.lastname: Rezayat
mesh_terms: D002352:Carrier Proteins / Q000494:pharmacology*; D045744:Cell Line, Tumor; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004717:Endometrium / Q000166:cytology; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006801:Humans; D007797:Laminin / Q000737:chemistry*; D057139:Nanofibers / Q000737:chemistry*; D010455:Peptides / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects; D014404:Tubulin / Q000235:genetics / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism; D000099176:Noggin Protein
chemical_list: D002352:Carrier Proteins; D005904:Glial Fibrillary Acidic Protein; D007797:Laminin; D010455:Peptides; D019253:Proto-Oncogene Proteins c-bcl-2; D014404:Tubulin; D014446:Tyrosine 3-Monooxygenase; D005680:gamma-Aminobutyric Acid; D000099176:Noggin Protein
keywords: BMHP-1; Laminin motif; Noggin; Self-assembling peptide nanofiber; Tyrosine hydroxylase