title: Knockdown of Tubulin Polymerization Promoting Protein Family Member 3 Suppresses Proliferation and Induces Apoptosis in Non-Small-Cell Lung Cancer.
abstract: Our previous studies demonstrated that depletion of tubulin polymerization promoting protein family member 3 (TPPP3) inhibits proliferation and induces apoptosis of HeLa cells. However, the expression and roles of TPPP3 in cancers remain largely unknown. In this study, we investigated the expression of TPPP3 in clinicopathological correlations in non-small-cell lung cancer (NSCLC) samples by immunohistochemistry. TPPP3 expression was significantly upregulated in NSCLC tissues, and high TPPP3 expression was positively associated with tumor size, lymph node metastasis, clinical stage, and poor survival. Furthermore, knockdown of TPPP3 by shRNA significantly inhibited cell proliferation and induced cell apoptosis and cell cycle arrest in vitro. In addition, depletion of TPPP3 inhibited lung cancer growth in vivo in the xenografts of H1299 cells; this effect was accompanied by the suppression of Ki67 expression. Our data suggested that TPPP3 might act as an oncogene in NSCLC. TPPP3 warrants consideration as a therapeutic candidate with anti-tumor potential.
authors.affiliation: 1. Department of Endocrinology and Metabolism, Institute of Endocrinology and Diabetology, Huashan Hospital, Fudan University, Shanghai, P.R. China; 2. Department of Medical Oncology, Shandong Cancer Hospital and Institute, Jinan, Shandong, P.R. China.
authors.forename: Yintao
authors.initials: Y
authors.lastname: Li
authors.affiliation: 3. Department of Pathology, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong, P.R. China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Xu
authors.affiliation: 1. Department of Endocrinology and Metabolism, Institute of Endocrinology and Diabetology, Huashan Hospital, Fudan University, Shanghai, P.R. China.
authors.forename: Kuanping
authors.initials: K
authors.lastname: Ye
authors.affiliation: 4. Department of Geriatrics, Zhongshan Hospital, Fudan University, Shanghai, P.R. China.
authors.forename: Nan
authors.initials: N
authors.lastname: Wu
authors.affiliation: 5. Department of Endocrinology, Renmin Hospital of Wuhan University, Wuhan, Hubei, P.R. China.
authors.forename: Junfeng
authors.initials: J
authors.lastname: Li
authors.affiliation: 1. Department of Endocrinology and Metabolism, Institute of Endocrinology and Diabetology, Huashan Hospital, Fudan University, Shanghai, P.R. China.
authors.forename: Naijia
authors.initials: N
authors.lastname: Liu
authors.affiliation: 1. Department of Endocrinology and Metabolism, Institute of Endocrinology and Diabetology, Huashan Hospital, Fudan University, Shanghai, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: He
authors.affiliation: 1. Department of Endocrinology and Metabolism, Institute of Endocrinology and Diabetology, Huashan Hospital, Fudan University, Shanghai, P.R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Lu
authors.affiliation: 1. Department of Endocrinology and Metabolism, Institute of Endocrinology and Diabetology, Huashan Hospital, Fudan University, Shanghai, P.R. China.
authors.forename: Wenbai
authors.initials: W
authors.lastname: Zhou
authors.affiliation: 1. Department of Endocrinology and Metabolism, Institute of Endocrinology and Diabetology, Huashan Hospital, Fudan University, Shanghai, P.R. China.
authors.forename: Renming
authors.initials: R
authors.lastname: Hu
keywords: NSCLC; TPPP3; cell apoptosis.; cell proliferation