title: In silico proposition to predict cluster of B- and T-cell epitopes for the usefulness of vaccine design from invasive, virulent and membrane associated proteins of C. jejuni.
abstract: OBJECTIVE
  Campylobacter jejuni is the one of the leading causes of bacterial diarrheal illness worldwide. This study aims to design specific epitopes for the utility of designing peptide vaccine(s) against C. jejuni by targeting invasive, virulent and membrane associated proteins like FlaA, Cia, CadF, PEB1, PEB3 and MOMP.
  
  
  METHODS
  In the present study, various immunoinformatics approaches have been applied to design a potential epitope based vaccine against C. jejuni. The tools include Bepipred, ABCpred, Immune Epitope databse (IEDB) resource portal, Autodock vina etc.
  
  
  RESULTS
  Peptides "EINKN", "TGSRLN", "KSNPDI", "LDENGCE" respectively from FlaA, MOMP, PEB3, CadF proteins were found to be the most potential B cell epitopes while peptides "FRINTNVAA", "NYFEGNLDM", "YKYSPKLNF", "YQDAIGLLV", "FRNNIVAFV" and "LIMPVFHEL" respectively from Fla, CadF, MOMP, PEB1A, PEB3 and Cia might elicit cell mediated immunity and "IFYTTGSRL" from MOMP protein might elicit both humoral and cell-mediated immunity. All these potential peptidic epitopes showed almost 80-100 % conservancy in different strains of C jejuni with varying proportions of population coverage ranging from 22-60 %. Further authentication of these peptide epitopes as probable vaccine candidate was mediated by their binding to specific HLA alleles using in silico docking technique.
  
  
  CONCLUSIONS
  Based on the present study, it could be concluded that these predicted epitopes might be used to design a vaccine against C. jejuni bacteria and thus, could be validated in model hosts to verify their efficacy as vaccine.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka-, 1000, Bangladesh.
authors.forename: Tahirah
authors.initials: T
authors.lastname: Yasmin
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka-, 1000, Bangladesh.
authors.forename: Salma
authors.initials: S
authors.lastname: Akter
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka-, 1000, Bangladesh.
authors.forename: Mouly
authors.initials: M
authors.lastname: Debnath
authors.affiliation: Laboratory of Applied Biochemistry, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Ebihara
authors.affiliation: Laboratory of Applied Biochemistry, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Nakagawa
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka-, 1000, Bangladesh. nabi@du.ac.bd.
authors.forename: A H M Nurun
authors.initials: AH
authors.lastname: Nabi
keywords: B-cell epitopes; Campylobacter jejuni; Epitopes; Guillain Barré syndrome; MHC Class I; MHC Class II; T-cell epitopes; Vaccine