title: [Usefulness of a rapid intrapartum real-time PCR assay in comparison with the group B Streptococcus culture screening at the end of pregnancy in pregnant women].
abstract: OBJECTIVE
  The objectives were to evaluate and compare the diagnostic accuracy of a rapid real-time PCR assay at the onset of labor with those of the current antenatal culture-based test at 34-38 weeks gestation for group B Streptococcus (GBS) screening.
  
  
  METHODS
  A prospective study including all pregnant women admitted for delivery after a 34-week gestation period was conducted in October 2012 at the Grenoble University Hospital Centre. A first culture-based GBS screening test was performed between 34 and 38 weeks of gestation followed by a second screening test at the onset of labor, using a real-time PCR Assay and a culture-based method (gold standard) in order to calculate the diagnostic accuracy.
  
  
  RESULTS
  One hundred an fifty-seven patients were enrolled. The sensitivity was 94.4% (95% CI, 72.7-99.9%) with intrapartum PCR assay and 50% (95% CI, 26-74%) with antepartum culture. Prevalence of GBS colonization was 7.6% with the antepartum culture method, 11.5% with intrapartum culture and 16.6% by using PCR-test.
  
  
  CONCLUSIONS
  Intrapartum PCR shows a much higher sensitivity compared to the antepartum culture-based screening mainly due to variations in GBS colonization and could allow us to target patients requiring intrapartum antibiotic prophylaxis more effectively.
authors.affiliation: Service de gynécologie et obstétrique et médecine de la reproduction, CHU de Grenoble, C.S. 10217, 38043 Grenoble cedex 9, France. Electronic address: marylinedefezbiot@gmail.com.
authors.forename: M
authors.initials: M
authors.lastname: Defez
authors.affiliation: Service de gynécologie et obstétrique et médecine de la reproduction, CHU de Grenoble, C.S. 10217, 38043 Grenoble cedex 9, France.
authors.forename: F
authors.initials: F
authors.lastname: Khizar
authors.affiliation: Laboratoire de bactériologie, département des agents infectieux, institut de biologie et de pathologie, CHU de Grenoble, C.S. 10217, 38043 Grenoble cedex 9, France; Université Joseph-Fourier, BP 53, 38041 Grenoble cedex 9, France.
authors.forename: M
authors.initials: M
authors.lastname: Maurin
authors.affiliation: Unité de bactériologie, UMR_MD1, institut de recherche biomédicale des armées, 91220 Brétigny-sur-Orge, France.
authors.forename: F
authors.initials: F
authors.lastname: Biot
authors.affiliation: Service de gynécologie et obstétrique et médecine de la reproduction, CHU de Grenoble, C.S. 10217, 38043 Grenoble cedex 9, France; Université Joseph-Fourier, BP 53, 38041 Grenoble cedex 9, France.
authors.forename: J-C
authors.initials: JC
authors.lastname: Pons
authors.affiliation: Service de gynécologie et obstétrique et médecine de la reproduction, CHU de Grenoble, C.S. 10217, 38043 Grenoble cedex 9, France; Université Joseph-Fourier, BP 53, 38041 Grenoble cedex 9, France.
authors.forename: F
authors.initials: F
authors.lastname: Sergent
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D007743:Labor, Obstetric; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis*; D060888:Real-Time Polymerase Chain Reaction / Q000592:standards*; D012680:Sensitivity and Specificity; D013290:Streptococcal Infections / Q000175:diagnosis*; D013997:Time Factors
keywords: Antenatal screening test; Dépistage anténatal; Grossesse; Group B Streptococcus; PCR en temps réel; Pregnancy; Real-time polymerase chain reaction; Streptocoque du groupe B