title: Telomere Visualization in Tissue Sections using Pyrrole-Imidazole Polyamide Probes.
abstract: Pyrrole-Imidazole (PI) polyamides bind to specific DNA sequences in the minor groove with high affinity. Specific DNA labeling by PI polyamides does not require DNA denaturation with harsh treatments of heat and formamide and has the advantages of rapid and less disruptive processing. Previously, we developed tandem hairpin PI polyamide probes (TH59 series), which label telomeres in cultured cell lines more efficiently than conventional methods, such as fluorescence in situ hybridization (FISH). Here, we demonstrate that a TH59 derivative, HPTH59-b, along with immunostaining for specifying cell types in the tissues, visualizes telomeres in mouse and human tissue sections. Quantitative measurements of telomere length with single-cell resolution suggested shorter telomeres in the proliferating cell fractions of tumor than in non-tumor tissues. Thus, PI polyamides are a promising alternative for telomere labeling in clinical research, as well as in cell biology.
authors.affiliation: Structural Biology Center, National Institute of Genetics, and Department of Genetics, Sokendai (Graduate University for Advanced Studies), Mishima, Shizuoka 411-8540, Japan.
authors.forename: Asuka
authors.initials: A
authors.lastname: Sasaki
authors.affiliation: Structural Biology Center, National Institute of Genetics, and Department of Genetics, Sokendai (Graduate University for Advanced Studies), Mishima, Shizuoka 411-8540, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Ide
authors.affiliation: Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo, Kyoto 606-8502, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kawamoto
authors.affiliation: Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo, Kyoto 606-8502, Japan.
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Bando
authors.affiliation: Department of Pathology, Hospital, National Center for Global Health and Medicine, Shinjuku, Tokyo 162-8655, Japan.
authors.forename: Yukinori
authors.initials: Y
authors.lastname: Murata
authors.affiliation: Department of Intractable Diseases, Research Institute, National Center for Global Health and Medicine, Shinjuku, Tokyo 162-8655, Japan.
authors.forename: Mari
authors.initials: M
authors.lastname: Shimura
authors.affiliation: Department of Surgery, Hospital, National Center for Global Health and Medicine, Shinjuku, Tokyo 162-8655, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Yamada
authors.affiliation: HiPep Laboratories, Nakatsukasa-cho 486-46, Kamigyo-ku, Kyoto 602-8158, Japan.
authors.forename: Akiyoshi
authors.initials: A
authors.lastname: Hirata
authors.affiliation: HiPep Laboratories, Nakatsukasa-cho 486-46, Kamigyo-ku, Kyoto 602-8158, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Nokihara
authors.affiliation: Division of Brain Function, Department of Integrated Genetics, National Institute of Genetics, and Department of Genetics, Sokendai, Mishima, Shizuoka 411-8540, Japan.
authors.forename: Tatsumi
authors.initials: T
authors.lastname: Hirata
authors.affiliation: Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo, Kyoto 606-8502, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sugiyama
authors.affiliation: Structural Biology Center, National Institute of Genetics, and Department of Genetics, Sokendai (Graduate University for Advanced Studies), Mishima, Shizuoka 411-8540, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Maeshima
mesh_terms: D000818:Animals; D003584:Cytological Techniques / Q000379:methods; D004247:DNA / Q000378:metabolism*; D006651:Histocytochemistry / Q000379:methods; D006801:Humans; D007093:Imidazoles / Q000378:metabolism*; D051379:Mice; D009757:Nylons / Q000378:metabolism*; D061848:Optical Imaging / Q000379:methods*; D011758:Pyrroles / Q000378:metabolism*; D013194:Staining and Labeling / Q000379:methods*; D016615:Telomere / Q000378:metabolism*
chemical_list: D007093:Imidazoles; D009757:Nylons; D011758:Pyrroles; C029899:imidazole; D004247:DNA