title: Long interspersed nuclear element (LINE)-1 methylation level as a molecular marker of early gastric cancer.
abstract: OBJECTIVE
  This study was performed to examine the state of long interspersed nuclear element (LINE)-1 methylation level in gastric epithelial dysplasias (GEDs) and evaluate as a molecular marker for gastric carcinogenesis when it was compared with RUNX3 expression.
  
  
  METHODS
  We examined 89 patients with GEDs subcategorized by the Vienna classification - 41 category 3 (low grade) and 48 category 4 (high grade/intramucosal carcinoma) lesion. All tissue samples were evaluated for RUNX3 immunohistochemical staining and the level of LINE-1 methylation.
  
  
  RESULTS
  The rate of negative expression of RUNX3 in category 4 lesion was significant higher than category 3 (P<0.01). LINE-1 methylation level was statistically different between category 3 and category 4 lesion (P<0.01). Between positive and negative expression of RUNX3 in GEDs, there was a significant difference of LINE-1 methylation level (P<0.01). The area under the ROC curve (AUC) of LINE-1 methylation level for diagnosis of category 4 lesion was 0.88 (95% CI, 0.76-1.00).
  
  
  CONCLUSIONS
  LINE-1 methylation level was well correlated with the Vienna classification of GED and it had a close relationship with the negative expression of RUNX3 in category 4 lesion. LINE-1 methylation level could be a good candidate for a molecular marker of early gastric cancer.
authors.affiliation: Department of Internal Medicine, St. Vincent's Hospital, the College of Medicine, the Catholic University of Korea, Seoul, South Korea.
authors.forename: Eun Jung
authors.initials: EJ
authors.lastname: Kim
authors.affiliation: Department of Internal Medicine, St. Vincent's Hospital, the College of Medicine, the Catholic University of Korea, Seoul, South Korea. Electronic address: jwchulkr@catholic.ac.kr.
authors.forename: Woo Chul
authors.initials: WC
authors.lastname: Chung
authors.affiliation: Department of Internal Medicine, St. Vincent's Hospital, the College of Medicine, the Catholic University of Korea, Seoul, South Korea.
authors.forename: Dae Bum
authors.initials: DB
authors.lastname: Kim
authors.affiliation: Department of Internal Medicine, St. Vincent's Hospital, the College of Medicine, the Catholic University of Korea, Seoul, South Korea.
authors.forename: Yeon-Ji
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: Department of Internal Medicine, St. Vincent's Hospital, the College of Medicine, the Catholic University of Korea, Seoul, South Korea.
authors.forename: Ji Min
authors.initials: JM
authors.lastname: Lee
authors.affiliation: Department of Pathology, St. Vincent's Hospital, the College of Medicine, the Catholic University of Korea, Seoul, South Korea.
authors.forename: Ji Han
authors.initials: JH
authors.lastname: Jung
authors.affiliation: Department of Pathology, Samsung Medical Center of Korea, Seoul, South Korea.
authors.forename: Yun Kyung
authors.initials: YK
authors.lastname: Lee
mesh_terms: D000368:Aged; D050677:Core Binding Factor Alpha 3 Subunit / Q000235:genetics*; D019175:DNA Methylation; D005260:Female; D005753:Gastric Mucosa / Q000473:pathology*; D006801:Humans; D020084:Long Interspersed Nucleotide Elements; D008297:Male; D008875:Middle Aged; D012372:ROC Curve; D056910:Republic of Korea; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D050677:Core Binding Factor Alpha 3 Subunit; C493729:Runx3 protein, human
keywords: Gastric cancer; Long interspersed nuclear element (LINE); RUNX3