title: Concomitant aneurysm detection in an intracranial dolichoectasia mouse model using a MicroFil polymer perfusion technique.
abstract: OBJECTIVE
  To assess the feasibility of using MicroFil polymer perfusion to detect concomitant saccular aneurysms in an intracranial arterial dolichoectasia (IADE) model in mice, and to report detailed histomorphometric features of these aneurysms.
  
  
  METHODS
  IADE models were created in C57/BL6 mice via microsurgical injection of 25 mU elastase into the cisterna magna. The cerebral vasculature was perfused with MicroFil polymer and harvested at 1, 3, and 7 days, and 2 and 4 weeks (n=8 for each group). IADE was defined by a tortuosity index >10 combined with a 25% increase in diameter of the A1 segment of the anterior cerebral artery (ACA), internal carotid artery (ICA), or basilar artery compared with the baseline of controls, which received heat-inactivated elastase. Saccular aneurysm occurrence rate, location, and morphological parameters were investigated using macroscopic and microscopic analysis.
  
  
  RESULTS
  IADE was present in 95% (36/38) of the subjects, with a mortality rate of 5% (2/40). Fifteen concomitant saccular aneurysms were detected in 8 (21%) of the 38 surviving mice, including 6 at the posterior communicating artery, 1 along the ACA, 2 along the anterior communicating artery complex, 3 along the ICA, and 3 along the middle cerebral artery. Rupture was confirmed in two aneurysms. Histological examination indicated that the aneurysms develop via arterial-wall remodelling, which is characterized by internal elastic lamina disruptions and muscular layer discontinuity in the media.
  
  
  CONCLUSIONS
  The proportion of subjects developing saccular aneurysms in addition to IADE in our mouse model is similar to the 15% of patients with IADE who have concomitant saccular aneurysms.
authors.affiliation: Department of Radiology, Mayo Clinic, Rochester, Minnesota, USA.|Department of Radiology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.
authors.forename: Yue-Qi
authors.initials: YQ
authors.lastname: Zhu
authors.affiliation: Department of Radiology, Mayo Clinic, Rochester, Minnesota, USA.
authors.forename: Da-Ying
authors.initials: DY
authors.lastname: Dai
authors.affiliation: Department of Radiology, Mayo Clinic, Rochester, Minnesota, USA.|Department of Pathology, Shanghai East Hospital, Medical School of Shanghai Tongji University, Shanghai, China.
authors.forename: Hai-Xia
authors.initials: HX
authors.lastname: Xing
authors.affiliation: Department of Radiology, Mayo Clinic, Rochester, Minnesota, USA.
authors.forename: Yong-Hong
authors.initials: YH
authors.lastname: Ding
authors.affiliation: Department of Radiology, Mayo Clinic, Rochester, Minnesota, USA.
authors.forename: Ramanathan
authors.initials: R
authors.lastname: Kadirvel
authors.affiliation: Department of Radiology, Mayo Clinic, Rochester, Minnesota, USA.
authors.forename: David F
authors.initials: DF
authors.lastname: Kallmes
mesh_terms: D000818:Animals; D020771:Anterior Cerebral Artery / Q000473:pathology / Q000601:surgery; D001488:Basilar Artery / Q000473:pathology / Q000601:surgery; D002343:Carotid Artery, Internal / Q000473:pathology / Q000601:surgery; D004195:Disease Models, Animal; D005260:Female; D002532:Intracranial Aneurysm / Q000139:chemically induced / Q000473:pathology* / Q000601:surgery; D051379:Mice; D008810:Mice, Inbred C57BL; D020768:Middle Cerebral Artery / Q000473:pathology / Q000601:surgery; D010196:Pancreatic Elastase / Q000633:toxicity; D010477:Perfusion / Q000379:methods*; D011108:Polymers / Q000008:administration & dosage; D012826:Silicone Elastomers / Q000008:administration & dosage*; D014715:Vertebrobasilar Insufficiency / Q000139:chemically induced / Q000473:pathology* / Q000601:surgery
chemical_list: D011108:Polymers; D012826:Silicone Elastomers; D010196:Pancreatic Elastase
keywords: Aneurysm; Blood Flow