title: Identification and Characterization of Acinetobacter sp. CNU961 Able to Grow with Phenol at High Concentrations.
abstract: An Acinetobacter sp., strain CNU961, with a higher tolerance to phenol was isolated, and identified through a set of taxonomic studies and a genetic complementation test. Enzymatic and mutagenic studies found that the strain dissimilate phenol by hydroxylation to catechol followed by an ortho-ring cleavage pathway to further mineralize it. The phenol hydroxylase, which is an inducible enzyme and requires NADPH for optimum activity, was not inhibited by phenol at concentrations up to 0.5 mM. The different kinetic behaviors of the enzyme activities on NADPH and on phenol reflected that the phenol hydroxylase of strain CNU961 is a multisubunit allosteric enzyme consisting of heterogeneous polypeptides.
authors.affiliation: a Department of Food Science and Technology, Institute of Biotechnology, Center for Specialized Agricultural Sciences, Chonnam National University.
authors.forename: K C
authors.initials: KC
authors.lastname: Jeong
authors.affiliation: a Department of Food Science and Technology, Institute of Biotechnology, Center for Specialized Agricultural Sciences, Chonnam National University.
authors.forename: E Y
authors.initials: EY
authors.lastname: Jeong
authors.affiliation: b Center for Specialized Agricultural Sciences, Division of Biological Resources, Chonnam National University.
authors.forename: T E
authors.initials: TE
authors.lastname: Hwang
authors.affiliation: a Department of Food Science and Technology, Institute of Biotechnology, Center for Specialized Agricultural Sciences, Chonnam National University.
authors.forename: S H
authors.initials: SH
authors.lastname: Choi
keywords: Acinetobacter sp.; catechol production; ortho-pathway; phenol hydroxylase; phenol tolerance