title: LysGH15 kills Staphylococcus aureus without being affected by the humoral immune response or inducing inflammation.
abstract: The lysin LysGH15, derived from the staphylococcal phage GH15, exhibits a wide lytic spectrum and highly efficient lytic activity against methicillin-resistant Staphylococcus aureus (MRSA). Here, we found that LysGH15 did not induce resistance in MRSA or methicillin-sensitive S. aureus (MSSA) strains after repeated treatment. Although LysGH15 triggered the generation of LysGH15-specific antibodies in mice, these antibodies did not block lytic activity in vitro (nor the binding capacity of LysGH15). More importantly, when the antibody titre was highest in mice immunized with LysGH15, a single intravenous injection of LysGH15 was sufficient to protect mice against lethal infection with MRSA. These results indicated that LysGH15-specific antibodies did not affect the killing efficiency of LysGH15 against MRSA in vitro or in vivo. LysGH15 also reduced pro-inflammatory cytokines in mice with lethal infections. Furthermore, a high-dose LysGH15 injection did not cause significant adverse effects or pathological changes in the main organs of treated animals. These results provide further evidence for the administration of LysGH15 as an alternative strategy for the treatment of infections caused by MRSA.
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, P. R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Immunology, College of Basic Medical Sciences, Jilin University, Changchun, 130021, P. R. China.
authors.forename: Dong
authors.initials: D
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, P. R. China.
authors.forename: Xinwei
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, P. R. China.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Hu
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, P. R. China.
authors.forename: Mengjun
authors.initials: M
authors.lastname: Cheng
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, P. R. China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Xia
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, P. R. China.
authors.forename: Pengjuan
authors.initials: P
authors.lastname: Gong
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, P. R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, P. R. China.
authors.forename: Jinli
authors.initials: J
authors.lastname: Ge
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, P. R. China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, P. R. China.
authors.forename: Ruopeng
authors.initials: R
authors.lastname: Cai
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, P. R. China.
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, P. R. China.
authors.forename: Changjiang
authors.initials: C
authors.lastname: Sun
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, P. R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Feng
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, P. R. China.
authors.forename: Liancheng
authors.initials: L
authors.lastname: Lei
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, P. R. China.|Jiangsu Co-innovation Center for the Prevention and Control of important Animal Infectious Disease and Zoonoses, Yangzhou 225009, P. R. China.
authors.forename: Wenyu
authors.initials: W
authors.lastname: Han
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, P. R. China.
authors.forename: Jingmin
authors.initials: J
authors.lastname: Gu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D005260:Female; D056724:Immunity, Humoral / Q000187:drug effects*; D007249:Inflammation / Q000139:chemically induced*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D009088:Mucoproteins / Q000494:pharmacology*; D013203:Staphylococcal Infections / Q000188:drug therapy; D013204:Staphylococcus Phages / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D009088:Mucoproteins; C010393:lysin, gastropoda