title: Protein N-terminal acetylation is required for embryogenesis in Arabidopsis.
abstract: Early embryonic development generates precursors of all major cell types in Arabidopsis. Among these precursors, the hypophysis divides asymmetrically to form the progenitors of the quiescent center and columella stem cells. A great deal has been learnt about the mechanisms that control the asymmetric division of the hypophysis and embryogenesis at the transcriptional level; however, no evidence of regulation at the co- or post-translational level has been reported. Here, we show that mutation of the catalytic subunit (Naa10) or auxiliary subunit (Naa15) of NatA, an N-terminal acetyltransferase that catalyzes protein N-terminal acetylation, produces an embryo-lethal phenotype. In addition, Naa10 and Naa15 were found to interact physically in planta Further analysis revealed that the observed embryonic patterning defects started at the early globular stage and that the asymmetric division of the hypophysis was irregular; thus, no quiescent center progenitor cells were generated in naa10 and naa15 embryos. We further observed that the polar distributions of auxin and its efflux carrier PIN1 were disturbed in naa10 embryos. Our results suggest that NatA is required for asymmetric division of the hypophysis and early embryonic patterning in Arabidopsis, and provides a link between protein N-terminal acetylation and embryogenesis in plants.
authors.affiliation: College of Life Sciences, Capital Normal University, and Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, Beijing Municipal Government; Beijing 100048, China.
authors.forename: Jinlin
authors.initials: J
authors.lastname: Feng
authors.affiliation: College of Life Sciences, Capital Normal University, and Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, Beijing Municipal Government; Beijing 100048, China.
authors.forename: Ruiqi
authors.initials: R
authors.lastname: Li
authors.affiliation: College of Life Sciences, Capital Normal University, and Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, Beijing Municipal Government; Beijing 100048, China.
authors.forename: Junya
authors.initials: J
authors.lastname: Yu
authors.affiliation: College of Life Sciences, Capital Normal University, and Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, Beijing Municipal Government; Beijing 100048, China.
authors.forename: Shuangshuang
authors.initials: S
authors.lastname: Ma
authors.affiliation: College of Life Sciences, Capital Normal University, and Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, Beijing Municipal Government; Beijing 100048, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Wu
authors.affiliation: College of Life Sciences, Capital Normal University, and Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, Beijing Municipal Government; Beijing 100048, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Life Sciences, Capital Normal University, and Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, Beijing Municipal Government; Beijing 100048, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Cao
authors.affiliation: College of Life Sciences, Capital Normal University, and Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, Beijing Municipal Government; Beijing 100048, China ligeng.ma@cnu.edu.cn.
authors.forename: Ligeng
authors.initials: L
authors.lastname: Ma
mesh_terms: D000107:Acetylation; D017360:Arabidopsis / Q000254:growth & development* / Q000378:metabolism; D047468:Immunoprecipitation; D063206:N-Terminal Acetyltransferases / Q000378:metabolism* / Q000502:physiology; D011499:Protein Processing, Post-Translational / Q000502:physiology; D012639:Seeds / Q000254:growth & development* / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D063206:N-Terminal Acetyltransferases
keywords: Arabidopsis; NatA; asymmetric division; embryogenesis; hypophysis; protein N-terminal acetylation.