title: A hemocyte-expressed fibrinogen-related protein gene (LvFrep) from the shrimp Litopenaeus vannamei: Expression analysis after microbial infection and during larval development.
abstract: Fibrinogen-related proteins (FREPs) comprise a large family of microbial recognition proteins involved in many biological functions in both vertebrate and invertebrate animals. By taking advantage of publicly accessible databases, we have identified a FREP-like homolog in the most cultivated penaeid shrimp, Litopenaeus vannamei (LvFrep). The obtained sequence showed a conserved fibrinogen-related domain (FReD) and displayed significant similarities to FREP-like proteins from other invertebrates and to ficolins from crustaceans. The expression of LvFrep appeared to be limited to circulating hemocytes. Interestingly, LvFrep gene expression was induced in shrimp hemocytes only in response to a Vibrio infection but not to the White spot syndrome virus (WSSV). Moreover, LvFrep transcript levels were detected early in fertilized eggs, suggesting the participation of this immune-related gene in the antimicrobial defenses during shrimp development.
authors.affiliation: Laboratory of Immunology Applied to Aquaculture, Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, 88040-900 Florianópolis, SC, Brazil.
authors.forename: Jaqueline da Rosa
authors.initials: JDR
authors.lastname: Coelho
authors.affiliation: Laboratory of Immunology Applied to Aquaculture, Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, 88040-900 Florianópolis, SC, Brazil.
authors.forename: Cairé
authors.initials: C
authors.lastname: Barreto
authors.affiliation: Laboratory of Immunology Applied to Aquaculture, Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, 88040-900 Florianópolis, SC, Brazil.
authors.forename: Amanda da Silva
authors.initials: ADS
authors.lastname: Silveira
authors.affiliation: Laboratory of Immunology Applied to Aquaculture, Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, 88040-900 Florianópolis, SC, Brazil.
authors.forename: Graziela Cleusa
authors.initials: GC
authors.lastname: Vieira
authors.affiliation: Laboratory of Immunology Applied to Aquaculture, Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, 88040-900 Florianópolis, SC, Brazil.
authors.forename: Rafael Diego
authors.initials: RD
authors.lastname: Rosa
authors.affiliation: Laboratory of Immunology Applied to Aquaculture, Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, 88040-900 Florianópolis, SC, Brazil. Electronic address: l.m.perazzolo@ufsc.br.
authors.forename: Luciane Maria
authors.initials: LM
authors.lastname: Perazzolo
mesh_terms: D000818:Animals; D005340:Fibrinogen / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D006434:Hemocytes / Q000378:metabolism; D010063:Ovum / Q000378:metabolism; D033561:Penaeidae / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000276:immunology*; D010802:Phylogeny; D020539:Sequence Analysis, Protein; D014733:Vibrio / Q000502:physiology*; D046848:White spot syndrome virus 1 / Q000502:physiology*
chemical_list: D005340:Fibrinogen
keywords: Crustacean immunity; Fibrinogen-related protein; Gene expression; Pattern recognition protein/receptor; Vibrio harveyi