title: Effects of Extracellular Polymeric Substance Composition on Bacteria Disinfection by Monochloramine: Application of MALDI-TOF/TOF-MS and Multivariate Analysis.
abstract: In our previous study, we reported that the transport of monochloramine is affected by the extracellular polymeric substance (EPS) composition, which in turn affects the cell viability of both biofilm and detached clusters.11 However, although the transport and reaction of monochloramine in biofilm could be observed, the specific biomolecules reacting with the disinfectant and the mechanism of disinfection remains elusive. In this study, the impact of EPS composition on bacteria disinfection by monochloramine was qualitatively determined using both wild-type and isogenic mutant Pseudomonas strains with different EPS-secretion capacity and composition. To evaluate their EPS reactivity and contribution to susceptibility to monochloramine, we investigated the bacteria disinfection process using Fourier transform infrared spectroscopy (FTIR) and matrix-assisted laser desorption-ionization time-of-flight/time-of-flight mass spectrometry (MALDI-TOF/TOF-MS). Canonical correlation analysis and partial least-squares regression modeling were employed to explore the changes that EPS underwent during the monochloramine disinfection process. The analyses results suggested significant reactions of the monochloramine with peptide fragments of proteins that are associated with carbohydrate utilization. Selected enzymes also showed different levels of inhibition by monochloramine when tested.
authors.forename: Kimberly M
authors.initials: KM
authors.lastname: Coburn
authors.forename: Qinzhe
authors.initials: Q
authors.lastname: Wang
authors.forename: Dustin
authors.initials: D
authors.lastname: Rediske
authors.forename: Ronald E
authors.initials: RE
authors.lastname: Viola
authors.forename: B Leif
authors.initials: BL
authors.lastname: Hanson
authors.forename: Zheng
authors.initials: Z
authors.lastname: Xue
authors.affiliation: Department of Chemical and Environmental Engineering, University of Toledo , Toledo, Ohio 43607, United States.
authors.forename: Youngwoo
authors.initials: Y
authors.lastname: Seo
mesh_terms: D001419:Bacteria; D004202:Disinfectants; D004203:Disinfection; D015999:Multivariate Analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D004202:Disinfectants