title: Studies of the binding mode of TXNHCH2COOH with calf thymus DNA by spectroscopic methods.
abstract: In this study, a thioxanthone derivative named 2-(9-oxo-9H-thioxanthen-2ylamino) acetic acid (TX-NHCH2COOH) was used to investigate small molecule and DNA binding interactions. Absorption and fluorescence emission spectroscopy were used and melting studies were used to explain the binding mode of TXNHCH2COOH-DNA. Intrinsic binding constant Kb TXNHCH2COOH was found 6×10(5)M(-1)from UV-Vis absorption spectroscopy. Fluorescence emmision intensity increased by adding ct-DNA to the TXNHCH2COOH and KI quenching experiments resulted with low Ksv value. Additionally, 3.7°C increase for Tm was observed. The observed quenching of EB and ct-DNA complex and increase viscosity values of ct-DNA by addition of TXNHCH2COOH was determined. All those results indicate that TXNHCH2COOH can intercalate into DNA base pairs. Fluorescence microscopy helped to display imaging of the TXNHCH2COOH-DNA solution.
authors.affiliation: Yildiz Technical University, Chemistry Department, Davutpasa Campus, 34220 Esenler, Istanbul, Turkey.
authors.forename: Nese
authors.initials: N
authors.lastname: Ataci
authors.affiliation: Yildiz Technical University, Chemistry Department, Davutpasa Campus, 34220 Esenler, Istanbul, Turkey. Electronic address: narsu@yildiz.edu.tr.
authors.forename: Nergis
authors.initials: N
authors.lastname: Arsu
mesh_terms: D000818:Animals; D002417:Cattle; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D007364:Intercalating Agents / Q000737:chemistry* / Q000494:pharmacology*; D009691:Nucleic Acid Denaturation / Q000187:drug effects; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013892:Thioxanthenes / Q000737:chemistry / Q000494:pharmacology; D044004:Xanthones / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D007364:Intercalating Agents; D013892:Thioxanthenes; D044004:Xanthones; D004247:DNA; C427203:calf thymus DNA; C484911:thioxanthone
keywords: Binding mode; Calf thymus DNA; Fluorescence image; Intercalation; Spectroscopy; Thioxanthone derivative