title: Targeted delivery of quercetin loaded mesoporous silica nanoparticles to the breast cancer cells.
abstract: Mesoporous silica nanoparticles (MSNs) have been promising vehicles for drug delivery. Quercetin (Q), a natural flavonoid, has been reported to have many useful effects. However, poor water solubility as well as less bioavailability has confined its use as a suitable anti-cancer drug. Therefore, profound approach is required to overcome these drawbacks.
  
  
  
  We have synthesized folic acid (FA) armed mesoporous silica nanoparticles (MSN-FA-Q) loaded with quercetin and then characterized it by DLS, SEM, TEM and FTIR. MTT, confocal microscopy, flow cytometry, scratch assay and immunoblotting were employed to assess the cell viability, cellular uptake, cell cycle arrest, apoptosis, wound healing and the expression levels of different signalling molecules in breast adenocarcinoma cells. Nanoparticle distribution was investigated by using ex vivo optical imaging and CAM assay was employed to assess tumor regression.
  
  
  
  MSN-FA-Q facilitates higher cellular uptake and allows more drug bioavailability to the breast cancer cells with over-expressed folate receptors. Our experimental results suggest that the newly synthesized MSN-FA-Q nanostructure caused cell cycle arrest and apoptosis in breast cancer cells through the regulation of Akt & Bax signalling pathways. Besides, we also observed that MSN-FA-Q has a concurrent anti-migratory role as well.
  
  
  
  This uniquely engineered quercetin loaded mesoporous silica nanoparticle ensures a targeted delivery with enhanced bioavailability.
  
  
  
  Effective targeted therapeutic strategy against breast cancer cells.
authors.affiliation: Department of Molecular Medicine, Bose Institute, Kolkata 700054, India.
authors.forename: Abhijit
authors.initials: A
authors.lastname: Sarkar
authors.affiliation: Department of Molecular Medicine, Bose Institute, Kolkata 700054, India.
authors.forename: Shatadal
authors.initials: S
authors.lastname: Ghosh
authors.affiliation: Department of Molecular Medicine, Bose Institute, Kolkata 700054, India.
authors.forename: Sayantani
authors.initials: S
authors.lastname: Chowdhury
authors.affiliation: Department of Molecular Medicine, Bose Institute, Kolkata 700054, India.
authors.forename: Bhawna
authors.initials: B
authors.lastname: Pandey
authors.affiliation: Department of Molecular Medicine, Bose Institute, Kolkata 700054, India. Electronic address: parames@jcbose.ac.in.
authors.forename: Parames C
authors.initials: PC
authors.lastname: Sil
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects; D016503:Drug Delivery Systems; D005260:Female; D005492:Folic Acid / Q000008:administration & dosage / Q000138:chemical synthesis / Q000737:chemistry; D006801:Humans; D061986:MCF-7 Cells; D051379:Mice; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D016062:Porosity; D011794:Quercetin / Q000008:administration & dosage* / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D023041:Xenograft Model Antitumor Assays
chemical_list: D012822:Silicon Dioxide; D005492:Folic Acid; D011794:Quercetin
keywords: Apoptosis; Biodistribution; Breast cancer; Ex-ovo; Folic acid; Nanoparticle