title: [The enhanced expression of Toll-like receptor 2 in CD8(+)T cells of chronic HBV infected patients].
abstract: Objective To detect the level of Toll-like receptor 2 (TLR2) in CD8(+)T cells of chronic HBV infected patients without antiviral treatment. Methods Forty chronic HBV infected donors without antivirus treatment and 19 healthy donors were enrolled in our study. All donors were divided into three groups: a high viral load (HBV DNA>1×10(4) copies/mL) group, a low viral load (HBV DNA>1×10(4) copies/mL) group, and a healthy control group. After the isolation and staining of peripheral blood mononuclear cells (PBMCs), the levels of TLR2, CD38, HLA-DR, CD95, programmed death-1 (PD-1) in CD8(+)T cells were detected by flow cytometry to further analyze the correlations between TLR2 and CD38, HLA-DR, CD95, PD-1. Results The expression of TLR2 in CD8(+)T cells of the HBV infected patients were significantly higher than that in the healthy donors. The expression of TLR2 in CD8(+)T cells of the low viral load group was significantly higher than that in the high viral load group. There were no correlations between the expression of TLR2 and the expressions of CD38, HLA-DR, CD95 and PD-1 in CD8(+)T cells in the chronic HBV infected patients. Conclusion The expression of TLR2 in CD8(+)T cells increases in chronic HBV infected patients.
authors.affiliation: Guangxi Key Laboratory for Complementary and Alternative Medicine Experimental Animal Models, Nanning 530001; Department of Immunology and Microbiology, Guangxi University of Traditional Chinese Medicine, Nanning 530001, China.
authors.forename: Chenxia
authors.initials: C
authors.lastname: Yun
authors.affiliation: Guangxi Key Laboratory for Complementary and Alternative Medicine Experimental Animal Models, Nanning 530001; Department of Immunology and Microbiology, Guangxi University of Traditional Chinese Medicine, Nanning 530001, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Department of Clinical Laboratory, Zhengzhou Children's Hospital, Zhengzhou 450000, China.
authors.forename: Di
authors.initials: D
authors.lastname: Kang
authors.affiliation: Guangxi Key Laboratory for Complementary and Alternative Medicine Experimental Animal Models, Nanning 530001; Department of Immunology, Guangxi Medical University, Nanning 530021, China.
authors.forename: Qihui
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Guangxi Key Laboratory for Complementary and Alternative Medicine Experimental Animal Models, Nanning 530001; Department of Immunology and Microbiology, Guangxi University of Traditional Chinese Medicine, Nanning 530001, China.
authors.forename: Lishan
authors.initials: L
authors.lastname: Peng
authors.affiliation: Guangxi Key Laboratory for Complementary and Alternative Medicine Experimental Animal Models, Nanning 530001; Department of Immunology and Microbiology, Guangxi University of Traditional Chinese Medicine, Nanning 530001, China.
authors.forename: Xian
authors.initials: X
authors.lastname: Liu
authors.affiliation: Guangxi Key Laboratory for Complementary and Alternative Medicine Experimental Animal Models, Nanning 530001; Department of Immunology and Microbiology, Guangxi University of Traditional Chinese Medicine, Nanning 530001, China. *Corresponding author, E-mail: lj986771558@163.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Leng
mesh_terms: D051997:ADP-ribosyl Cyclase 1 / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D000368:Aged; D001782:Blood Donors; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism* / Q000821:virology; D002648:Child; D005260:Female; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000378:metabolism; D006515:Hepatitis B virus / Q000502:physiology; D019694:Hepatitis B, Chronic / Q000097:blood / Q000378:metabolism* / Q000821:virology; D054884:Host-Pathogen Interactions; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism / Q000821:virology; D008297:Male; D008875:Middle Aged; D061026:Programmed Cell Death 1 Receptor / Q000378:metabolism; D051195:Toll-Like Receptor 2 / Q000378:metabolism*; D019562:Viral Load; D055815:Young Adult; D019014:fas Receptor / Q000378:metabolism
chemical_list: D006684:HLA-DR Antigens; C105992:PDCD1 protein, human; D061026:Programmed Cell Death 1 Receptor; D051195:Toll-Like Receptor 2; D019014:fas Receptor; D051997:ADP-ribosyl Cyclase 1