title: Long-term Observation of Regenerated Periodontium Induced by FGF-2 in the Beagle Dog 2-Wall Periodontal Defect Model.
abstract: The long-term stability and qualitative characteristics of periodontium regenerated by FGF-2 treatment were compared with normal physiological healing tissue controls in a Beagle dog 2-wall periodontal defect model 13 months after treatment by assessing tissue histology and three-dimensional microstructure using micro-computed tomography (μCT). After FGF-2 (0.3%) or vehicle treatment at the defect sites, serial changes in the bone mineral content (BMC) were observed using periodic X-ray imaging. Tissues were harvested at 13 months, evaluated histomorphometrically, and the cortical bone volume and trabecular bone structure of the newly formed bone were analyzed using μCT. FGF-2 significantly increased the BMC of the defect area at 2 months compared with that of the control group, and this difference was unchanged through 13 months. The cortical bone volume was significantly increased by FGF-2, but there was no difference between the groups in trabecular bone structure. Bone maturation was occurring in both groups because of the lower cortical volume and denser trabecular bone than what is found in intact bone. FGF-2 also increased the area of newly formed bone as assessed histomorphometrically, but the ratios of trabecular bone in the defect area were similar between the control and FGF-2 groups. These results suggest that FGF-2 stimulates neogenesis of alveolar bone that is of similar quality to that of the control group. The lengths of the regenerated periodontal ligament and cementum, measured as the distance from the defect bottom to the apical end of the gingival epithelium, and height and area of the newly formed bone in the FGF-2 group were larger than those in the control group. The present study demonstrated that, within the limitation of artificial periodontal defect model, the periodontal tissue regenerated by FGF-2 was maintained for 13 months after treatment and was qualitatively equivalent to that generated through the physiological healing process.
authors.affiliation: Pharmacology Department, Drug Research Center, Kaken Pharmaceutical Co., LTD, Yamashina-ku, Kyoto, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Anzai
authors.affiliation: Pharmacology Department, Drug Research Center, Kaken Pharmaceutical Co., LTD, Yamashina-ku, Kyoto, Japan.|Department of Periodontology, Osaka University Graduate School of Dentistry, Suita, Osaka, Japan.
authors.forename: Toshie
authors.initials: T
authors.lastname: Nagayasu-Tanaka
authors.affiliation: Pharmacology Department, Drug Research Center, Kaken Pharmaceutical Co., LTD, Yamashina-ku, Kyoto, Japan.
authors.forename: Akio
authors.initials: A
authors.lastname: Terashima
authors.affiliation: Pharmacology Department, Drug Research Center, Kaken Pharmaceutical Co., LTD, Yamashina-ku, Kyoto, Japan.
authors.forename: Taiji
authors.initials: T
authors.lastname: Asano
authors.affiliation: Department of Periodontology, Osaka University Graduate School of Dentistry, Suita, Osaka, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Yamada
authors.affiliation: Department of Periodontology, Osaka University Graduate School of Dentistry, Suita, Osaka, Japan.
authors.forename: Takenori
authors.initials: T
authors.lastname: Nozaki
authors.affiliation: Department of Periodontology, Osaka University Graduate School of Dentistry, Suita, Osaka, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kitamura
authors.affiliation: Department of Periodontology, Osaka University Graduate School of Dentistry, Suita, Osaka, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Murakami
mesh_terms: D000818:Animals; D015519:Bone Density / Q000502:physiology; D001842:Bone and Bones / Q000187:drug effects / Q000473:pathology; D004285:Dogs; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D008336:Mandibular Diseases / Q000209:etiology / Q000378:metabolism*; D010513:Periodontal Ligament / Q000187:drug effects*; D010519:Periodontium / Q000187:drug effects* / Q000473:pathology*
chemical_list: D016222:Fibroblast Growth Factor 2