title: Generation of functional hepatocyte-like cells from human deciduous periodontal ligament stem cells.
abstract: Human deciduous periodontal ligament stem cells have been introduced for as an easily accessible source of stem cells from dental origin. Although recent studies have revealed the ability of these stem cells in multipotential attribute, their efficiency of hepatic lineage differentiation has not been addressed so far. The aim of this study is to investigate hepatic lineage fate competence of periodontal ligament stem cells through direct media induction. Differentiation of periodontal ligament stem cells into hepatocyte-like cells was conducted by the exposure of two phase media induction. First phase was performed in the presence of hepatocyte growth factors to induce a definitive endoderm formation. In the subsequent phase, the cells were treated with oncostatin M and dexamethosone followed by insulin and transferrin to generate hepatocyte-like cells. Hepatic-related characters of the generated hepatocyte-like cells were determined at both mRNA and protein level followed by functional assays. Foremost changes observed in the generation of hepatocyte-like cells were the morphological features in which these cells were transformed from fibroblastic shape to polygonal shape. Temporal expression of hepatic markers ranging from early endodermal up to late markers were detected in the hepatocyte-like cells. Crucial hepatic markers such as glycogen storage, albumin, and urea secretion were also shown. These findings exhibited the ability of periodontal ligament stem cells of dental origin to be directed into hepatic lineage fate. These cells can be regarded as an alternative autologous source in the usage of stem cell-based treatment for liver diseases.
authors.affiliation: Department of Peadiatric Dentistry and Orthodontics, Faculty of Dentistry, University of Malaya, 50603, Kuala Lumpur, Malaysia. srinitha_37@yahoo.com.my.
authors.forename: Punitha
authors.identifier: http://orcid.org/0000-0002-6662-6538
authors.initials: P
authors.lastname: Vasanthan
authors.affiliation: Department of Peadiatric Dentistry and Orthodontics, Faculty of Dentistry, University of Malaya, 50603, Kuala Lumpur, Malaysia.
authors.forename: Pukana
authors.initials: P
authors.lastname: Jayaraman
authors.affiliation: Department of Peadiatric Dentistry and Orthodontics, Faculty of Dentistry, University of Malaya, 50603, Kuala Lumpur, Malaysia.
authors.forename: Wijenthiran
authors.initials: W
authors.lastname: Kunasekaran
authors.affiliation: Department of Peadiatric Dentistry and Orthodontics, Faculty of Dentistry, University of Malaya, 50603, Kuala Lumpur, Malaysia.
authors.forename: Anthony
authors.initials: A
authors.lastname: Lawrence
authors.affiliation: Department of Restorative Dentistry, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Nareshwaran
authors.initials: N
authors.lastname: Gnanasegaran
authors.affiliation: cGMP compliance Stem Cells Laboratory, Hygieia Innovation Sdn Bhd, Lot 1G-2G, Lanai Complex No.2, Persiaran Seri Perdana, Presint 10, 62250, Putrajaya, Malaysia.
authors.forename: Vijayendran
authors.initials: V
authors.lastname: Govindasamy
authors.affiliation: Department of Peadiatric Dentistry and Orthodontics, Faculty of Dentistry, University of Malaya, 50603, Kuala Lumpur, Malaysia. sabrim@um.edu.my.
authors.forename: Sabri
authors.initials: S
authors.lastname: Musa
authors.affiliation: Department of Restorative Dentistry, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Noor Hayaty Abu
authors.initials: NH
authors.lastname: Kasim
mesh_terms: D015415:Biomarkers / Q000032:analysis; D002454:Cell Differentiation / Q000187:drug effects; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology*; D018507:Gene Expression Regulation, Developmental; D022781:Hepatocytes / Q000166:cytology*; D006801:Humans; D010513:Periodontal Ligament / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects
chemical_list: D015415:Biomarkers; D003470:Culture Media
keywords: Cell differentiation; Dental stem cells; Periodontal ligament