title: High hydrostatic pressure leads to free radicals accumulation in yeast cells triggering oxidative stress.
abstract: Saccharomyces cerevisiae is a unicellular organism that during the fermentative process is exposed to a variable environment; hence, resistance to multiple stress conditions is a desirable trait. The stress caused by high hydrostatic pressure (HHP) in S. cerevisiae resembles the injuries generated by other industrial stresses. In this study, it was confirmed that gene expression pattern in response to HHP displays an oxidative stress response profile which is expanded upon hydrostatic pressure release. Actually, reactive oxygen species (ROS) concentration level increased in yeast cells exposed to HHP treatment and an incubation period at room pressure led to a decrease in intracellular ROS concentration. On the other hand, ethylic, thermic and osmotic stresses did not result in any ROS accumulation in yeast cells. Microarray analysis revealed an upregulation of genes related to methionine metabolism, appearing to be a specific cellular response to HHP, and not related to other stresses, such as heat and osmotic stresses. Next, we investigated whether enhanced oxidative stress tolerance leads to enhanced tolerance to HHP stress. Overexpression of STF2 is known to enhance tolerance to oxidative stress and we show that it also leads to enhanced tolerance to HHP stress.
authors.affiliation: Núcleo de Biotecnologia, Centro de Ciências da Saúde, Universidade Federal do Espírito Santo, Vitória, ES, 29040-090, Brazil.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Bravim
authors.affiliation: Núcleo de Biotecnologia, Centro de Ciências da Saúde, Universidade Federal do Espírito Santo, Vitória, ES, 29040-090, Brazil.
authors.forename: Mainã M
authors.initials: MM
authors.lastname: Mota
authors.affiliation: Núcleo de Biotecnologia, Centro de Ciências da Saúde, Universidade Federal do Espírito Santo, Vitória, ES, 29040-090, Brazil.
authors.forename: A Alberto R
authors.initials: AA
authors.lastname: Fernandes
authors.affiliation: Núcleo de Biotecnologia, Centro de Ciências da Saúde, Universidade Federal do Espírito Santo, Vitória, ES, 29040-090, Brazil patricia.fernandes@ufes.br.
authors.forename: Patricia M B
authors.initials: PM
authors.lastname: Fernandes
mesh_terms: D005609:Free Radicals / Q000378:metabolism*; D020869:Gene Expression Profiling; D006358:Hot Temperature; D006874:Hydrostatic Pressure; D046228:Microarray Analysis; D009997:Osmotic Pressure; D018384:Oxidative Stress; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D013312:Stress, Physiological
chemical_list: D005609:Free Radicals
keywords: Saccharomyces cerevisiae; environmental stresses; gene expression; high hydrostatic pressure; oxidative stress; reactive oxygen species