title: Open encapsulation-vitrification for cryopreservation of algae.
abstract: Vitrification offers a cost-effective solution for the preservation and management of genetic resources with, low-cost international movement of selected genetic materials and for long-term maintenance of stable stocks of a wide variety of microorganisms. However, its success is limited by the wide range of algal species. Here, we report a simple open encapsulation-vitrification protocol of cryopreservation. Results showed that ∼58% and ∼27% of Oocystis sp. survived vitrification-warming after the open and closed system of vitrification respectively when compared to non-cryopreserved controls. The improved success in an open system of vitrification was also observed in Anabaena sp. Furthermore, with the addition of 2-mercaptoethanol or glutathione the post-warming viability of vitrified algae in both open and closed system of vitrification was significantly improved (p < 0.05). The present case study aimed to develop a vitrification-based cryopreservation protocol and confirms an improvement in survival percentage over conventional encapsulation-vitrification method.
authors.affiliation: Department of Chemical Engineering, Rourkela, Odisha, India. Electronic address: namratajaiswal@gmail.com.
authors.forename: Namrata
authors.initials: N
authors.lastname: Kumari
authors.affiliation: Biotechnology and Medical Engineering, National Institute of Technology, Rourkela, Odisha, India.
authors.forename: Mukesh Kumar
authors.initials: MK
authors.lastname: Gupta
authors.affiliation: Department of Chemical Engineering, Rourkela, Odisha, India.
authors.forename: Raghubansh Kumar
authors.initials: RK
authors.lastname: Singh
mesh_terms: D000460:Chlorophyta; D015925:Cryopreservation / Q000379:methods*; D000458:Cyanobacteria; D058989:Vitrification
keywords: Algae; Anabaena; Cryopreservation; Microalgae; Oocystis; Vitrification