title: Cationic Two-Photon Lanthanide Bioprobes Able to Accumulate in Live Cells.
abstract: An original cationic water-soluble cyclen-based Eu(III) complex [EuL(1)](+) featuring a chromophore-functionalized antenna to increase the two-photon (2P) absorption properties was synthesized. The photophysical properties were thoroughly studied in various solvents and rationalized with the help of theoretical calculations. The complex exhibits an optimized 2P absorption cross section. Finally, 2P microscopy imaging experiments on living T24 human cancer cells highlighted the spontaneous internalization and the biological stability of this 2P bioprobe in vitro. Macrocyclic-based antennas open new perspectives for future optimization of the photophysical properties and allows envisaging the design of Eu, Tb, Yb, and Sm bioprobes. This result also opens the way for the design of functional two-photon Ln complexes able to monitor intracellular physicochemical parameters.
authors.affiliation: Univ Lyon, ENS Lyon, CNRS, Université Lyon 1, Laboratoire de Chimie, UMR 5182 , 46 allée d'Italie, 69364 Lyon, France.
authors.forename: Anh Thy
authors.initials: AT
authors.lastname: Bui
authors.affiliation: Université de Brest, UMR-CNRS 6521, UFR des Sciences et Techniques 6 avenue Victor le Gorgeu, C.S. 93837, 29238, Brest Cedex 3, France.
authors.forename: Maryline
authors.initials: M
authors.lastname: Beyler
authors.affiliation: Univ Lyon, ENS Lyon, CNRS, Université Lyon 1, Laboratoire de Chimie, UMR 5182 , 46 allée d'Italie, 69364 Lyon, France.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Liao
authors.affiliation: INSERM, U1209, Université Grenoble Alpes, IAB , F-38000 Grenoble, France.
authors.forename: Alexei
authors.initials: A
authors.lastname: Grichine
authors.affiliation: INSERM, U1209, Université Grenoble Alpes, IAB , F-38000 Grenoble, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Duperray
authors.affiliation: Univ Lyon, ENS Lyon, CNRS, Université Lyon 1, Laboratoire de Chimie, UMR 5182 , 46 allée d'Italie, 69364 Lyon, France.
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Mulatier
authors.affiliation: Institut des Sciences Chimiques de Rennes, UMR 6226 CNRS, Université de Rennes 1 , 263 Avenue du Général Leclerc, 35042 Rennes Cedex, France.
authors.forename: Boris Le
authors.initials: BL
authors.lastname: Guennic
authors.affiliation: Univ Lyon, ENS Lyon, CNRS, Université Lyon 1, Laboratoire de Chimie, UMR 5182 , 46 allée d'Italie, 69364 Lyon, France.
authors.forename: Chantal
authors.initials: C
authors.lastname: Andraud
authors.affiliation: Univ Lyon, ENS Lyon, CNRS, Université Lyon 1, Laboratoire de Chimie, UMR 5182 , 46 allée d'Italie, 69364 Lyon, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Maury
authors.affiliation: Université de Brest, UMR-CNRS 6521, UFR des Sciences et Techniques 6 avenue Victor le Gorgeu, C.S. 93837, 29238, Brest Cedex 3, France.
authors.forename: Raphaël
authors.initials: R
authors.lastname: Tripier
mesh_terms: D002412:Cations; D028581:Lanthanoid Series Elements / Q000378:metabolism*; D008853:Microscopy / Q000379:methods; D015335:Molecular Probes / Q000378:metabolism*
chemical_list: D002412:Cations; D028581:Lanthanoid Series Elements; D015335:Molecular Probes