title: Cryopreservation of in vitro shoot tips of strawberry by the vitrification method - establishment of a duplicate collection of Fragaria germplasm.
abstract: BACKGROUND
  The National German Strawberry Genebank, which includes 183 cultivars and 270 accessions of wild Fragaria species, is maintained in a field collection of the Fruit Genebank at Dresden-Pillnitz.
  
  
  OBJECTIVE
  A duplicate collection stored in liquid nitrogen would provide a higher level of security for these irreplaceable genetic resources.
  
  
  METHODS
  Four distinct cryopreservation protocols were tested earlier and the best method, a PVS2 vitrification method with a 14-day alternating-temperature cold acclimation, was modified for further genotype screening.
  
  
  RESULTS
  A comprehensive genotype spectrum was tested by screening 107 Fragaria ×ananassa cultivars and 20 Fragaria wild species (51 accessions). The average recovery for the cultivars using the optimized medium was 89.55 % and for the wild species 85.50 %.
  
  
  CONCLUSIONS
  These results indicate that the method could be used to establish a backup collection in liquid nitrogen. Meristem cultures will be established and the collection will be cryopreserved using this vitrification technique to provide cost effective long-term storage.
authors.affiliation: Julius Kuhn-Institute, Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Fruit Crops, Dresden, Germany. monika.hoefer@jki.bund.de.
authors.forename: Monika
authors.initials: M
authors.lastname: Höfer
mesh_terms: D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D031985:Fragaria / Q000235:genetics; D005838:Genotype; D018520:Plant Shoots; D000068098:Seed Bank; D058989:Vitrification
chemical_list: D003451:Cryoprotective Agents