title: Astragaloside IV Inhibits the Inflammatory Injury of Chicken Type II Pneumocytes Induced by Avian Pathogenic Escherichia coli.
abstract: Avian pathogenic Escherichia coli (APEC) induces septicemia in chickens by invading type II pneumocytes after breaching the blood-air barrier. Type II pneumocytes play an important role in maintaining the function of the blood-air barrier. Astragaloside IV has been shown in previous studies to have an anti-inflammatory effect. To explore whether astragaloside IV can inhibit APEC-induced injury in chicken type II pneumocytes, cells were infected with APEC-O78. The results showed that astragaloside IV significantly reduced cell damage in chicken type II pneumocytes induced by APEC-O78 by downregulating the production of TNF-α and IL-1β, upregulating the secretion of IL-4 and IL-10, suppressing the mRNA levels of TLR-4, TLR-5, ERK, and p38 of chicken type II pneumocytes as well as inhibiting bacterial adhesion and F-actin cytoskeleton polymerization. These results suggest that astragaloside IV may be useful in novel pharmaco-therapeutic approaches to the treatment of chicken colibacillosis.
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, Jilin, 130062, China.
authors.forename: Li-Yan
authors.initials: LY
authors.lastname: Zhang
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, Jilin, 130062, China.
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Yi
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, Jilin, 130062, China.
authors.forename: Xun
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, Jilin, 130062, China.
authors.forename: Shuai-Cheng
authors.initials: SC
authors.lastname: Wu
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, Jilin, 130062, China.
authors.forename: Yun-Xing
authors.initials: YX
authors.lastname: Fu
authors.affiliation: Yunfu Quantity and Metrology Supervision Testing Institute of Guangdong Province, 1 Fushan Road, Yunfu, Guangdong, 527300, China.
authors.forename: Cui
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, Jilin, 130062, China. fubd@jlu.edu.cn.
authors.forename: Ben-Dong
authors.initials: BD
authors.lastname: Fu
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, Jilin, 130062, China.
authors.forename: Hai-Qing
authors.initials: HQ
authors.lastname: Shen
authors.affiliation: Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun, Jilin, 130062, China.
authors.forename: Xu-Bin
authors.initials: XB
authors.lastname: Wei
mesh_terms: D000199:Actins / Q000378:metabolism; D056809:Alveolar Epithelial Cells / Q000187:drug effects / Q000473:pathology*; D000818:Animals; D001422:Bacterial Adhesion; D002645:Chickens; D016207:Cytokines / Q000235:genetics; D004926:Escherichia coli / Q000472:pathogenicity*; D004927:Escherichia coli Infections; D005786:Gene Expression Regulation / Q000187:drug effects; D007249:Inflammation / Q000188:drug therapy / Q000517:prevention & control*; D018836:Inflammation Mediators / Q000378:metabolism; D012503:Saponins / Q000494:pharmacology* / Q000627:therapeutic use; D014315:Triterpenes / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D000199:Actins; D016207:Cytokines; D018836:Inflammation Mediators; D012503:Saponins; D014315:Triterpenes; C052064:astragaloside A
keywords: astragaloside IV; avian pathogenic Escherichia coli; chicken type II pneumocyte; injury