title: Protective Effects of Intralipid and Caffeic Acid Phenethyl Ester (CAPE) on Hepatotoxicity and Pancreatic Injury Caused by Dichlorvos in Rats.
abstract: The present study was aimed to the investigate the protective effects of caffeic acid phenethyl ester (CAPE) and intralipid (IL) on hepatotoxicity and pancreatic injury caused by acute dichlorvos (D) intoxication in rats. Forty-eight Wistar rats were randomly divided into seven groups each containing seven rats except control groups. The groups included control, D, CAPE, IL, D + CAPE, D + IL, and D + CAPE + IL. Total antioxidant status and total oxidative stress levels were measured by automated colorimetric assay. Tissues were evaluated using hematoxylin and eosin (H&E) staining. Tissues were analyzed with hematoxylin and eosin by using standard protocols. Also, Bcl-2, Bax and caspase-3 were evaluated by immunohistochemical method in liver tissue. Total oxidant status in control, CAPE, and IL groups were significantly lower, and total antioxidant status in the D + CAPE, D + IL, and D + IL + CAPE groups were significantly higher compared to the D group. CAPE and IL treatment decreased the apoptotic and mitotic cell count in liver tissue. Parenchymal necrosis caused by dichlorvos is observed in pancreas tissues of rats. Mild congestion and edema formation occurred in pancreas tissues following D + CAPE and D + IL therapies. These results indicate that CAPE and IL have the potential to decrease oxidative stress and hepatic and pancreatic injuries caused by acute dichlorvos intoxication. These drugs can be considered as a new method for supportive and protective therapy against pesticide intoxication.
authors.affiliation: Department of Pharmacology, School of Medicine, Mustafa Kemal University, Hatay, Turkey.
authors.forename: Harun
authors.initials: H
authors.lastname: Alp
authors.affiliation: Department of Pharmacology, School of Medicine, Mustafa Kemal University, Hatay, Turkey.
authors.forename: Neslihan
authors.initials: N
authors.lastname: Pinar
authors.affiliation: Department of Medical Physiology, School of Medicine, Mustafa Kemal University, Hatay, Turkey. drecepfatih@gmail.com.|Department of Molecular Biochemistry and Genetics, School of Medicine, Mustafa Kemal University, Hatay, Turkey. drecepfatih@gmail.com.
authors.forename: Recep
authors.initials: R
authors.lastname: Dokuyucu
authors.affiliation: Department of Emergency Medicine, School of Medicine, Mustafa Kemal University, Hatay, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Sahan
authors.affiliation: Department of General Surgery, School of Medicine, Mustafa Kemal University, Hatay, Turkey.
authors.forename: Cem
authors.initials: C
authors.lastname: Oruc
authors.affiliation: Department of Medical Biochemistry, School of Medicine, Dicle University, Diyarbakır, Turkey.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Kaplan
authors.affiliation: Department of Medical Pathology, School of Medicine, Medeniyet University, Istanbul, Turkey.
authors.forename: Serkan
authors.initials: S
authors.lastname: Senol
authors.affiliation: Department of Medical Pathology, School of Medicine, Medeniyet University, Istanbul, Turkey.
authors.forename: Ayse Bahar
authors.initials: AB
authors.lastname: Ceyran
mesh_terms: D000818:Animals; D002109:Caffeic Acids / Q000008:administration & dosage* / Q000494:pharmacology; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism / Q000517:prevention & control*; D004006:Dichlorvos / Q000633:toxicity*; D004655:Emulsions / Q000008:administration & dosage / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D010182:Pancreatic Diseases / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D010626:Phenylethyl Alcohol / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D010743:Phospholipids / Q000008:administration & dosage* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013024:Soybean Oil / Q000008:administration & dosage* / Q000494:pharmacology; D016896:Treatment Outcome
chemical_list: D002109:Caffeic Acids; D004655:Emulsions; D010743:Phospholipids; C545823:soybean oil, phospholipid emulsion; D004006:Dichlorvos; D013024:Soybean Oil; C055494:caffeic acid phenethyl ester; D010626:Phenylethyl Alcohol
keywords: CAPE; Dichlorvos; Hepatotoxicity; Intralipid; Organophosphate pesticide; Oxidative stress; Pancreatic injury