title: Inverted repeat Alu elements in the human lincRNA-p21 adopt a conserved secondary structure that regulates RNA function.
abstract: LincRNA-p21 is a long intergenic non-coding RNA (lincRNA) involved in the p53-mediated stress response. We sequenced the human lincRNA-p21 (hLincRNA-p21) and found that it has a single exon that includes inverted repeat Alu elements (IRAlus). Sense and antisense Alu elements fold independently of one another into a secondary structure that is conserved in lincRNA-p21 among primates. Moreover, the structures formed by IRAlus are involved in the localization of hLincRNA-p21 in the nucleus, where hLincRNA-p21 colocalizes with paraspeckles. Our results underscore the importance of IRAlus structures for the function of hLincRNA-p21 during the stress response.
authors.affiliation: Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06511, USA.|Howard Hughes Medical Institute, Chevy Chase, MD 20815, USA.
authors.forename: Isabel
authors.identifier: http://orcid.org/0000-0002-3107-8738
authors.initials: I
authors.lastname: Chillón
authors.affiliation: Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06511, USA anna.pyle@yale.edu.|Howard Hughes Medical Institute, Chevy Chase, MD 20815, USA.|Department of Chemistry, Yale University, New Haven, CT 06511, USA.
authors.forename: Anna M
authors.initials: AM
authors.lastname: Pyle
mesh_terms: D020087:Alu Elements; D000818:Animals; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D017124:Conserved Sequence; D023061:Gene Order; D006801:Humans; D055029:Inverted Repeat Sequences; D051379:Mice; D009690:Nucleic Acid Conformation; D034443:RNA Transport; D062085:RNA, Long Noncoding / Q000737:chemistry* / Q000235:genetics*; D013312:Stress, Physiological; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
chemical_list: D062085:RNA, Long Noncoding; D016159:Tumor Suppressor Protein p53