title: Co-transplantation of MRF-overexpressing oligodendrocyte precursor cells and Schwann cells promotes recovery in rat after spinal cord injury.
abstract: Oligodendrocyte (OL) replacement is a promising treatment strategy for spinal cord injury (SCI). However, the poor survival of transplanted OLs or their precursors and inhibition of axonal regeneration are two major challenges with this approach. Our previous study showed that Schwann cells (SCs) promoted survival, proliferation, and migration of transplanted OL progenitor cells (OPCs) and neurological recovery. Remyelination is an important basis for functional recovery following spinal cord injury. It has been reported that myelin gene regulatory factor (MRF), a transcriptional regulator which specifically is expressed in postmitotic OLs within the CNS, is essential for OL maturation and CNS myelination. In the present study, we investigated whether co-transplantation of MRF-overexpressing OPCs (MRF-OPCs) and SCs could improve functional recovery in a rat model of contusional SCI. MRF overexpression had no effect on OPC survival or migration, but stimulated the differentiation of OPCs both in vitro and in vivo. Co-transplantation of MRF-OPCs and SCs increased myelination and tissue repair after SCI, leading to the recovery of neurological function. These results indicate that co-transplantation of MRF-OPCs and SCs may be an effective treatment strategy for SCI.
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical College, 287 Chang Huai Road, Bengbu 233004, PR China; Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, 287 Chang Huai Road, Bengbu 233004, PR China.
authors.forename: Xiu-Mei
authors.initials: XM
authors.lastname: Xie
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical College, 287 Chang Huai Road, Bengbu 233004, PR China; Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, 287 Chang Huai Road, Bengbu 233004, PR China.
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: Shi
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical College, 287 Chang Huai Road, Bengbu 233004, PR China; Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, 287 Chang Huai Road, Bengbu 233004, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Shen
authors.affiliation: Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, 287 Chang Huai Road, Bengbu 233004, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.affiliation: Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, 287 Chang Huai Road, Bengbu 233004, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Qi
authors.affiliation: Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, 287 Chang Huai Road, Bengbu 233004, PR China.
authors.forename: Qi-Yi
authors.initials: QY
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical College, 287 Chang Huai Road, Bengbu 233004, PR China.
authors.forename: Lun-Jun
authors.initials: LJ
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical College, 287 Chang Huai Road, Bengbu 233004, PR China; Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, 287 Chang Huai Road, Bengbu 233004, PR China.
authors.forename: He-Zuo
authors.initials: HZ
authors.lastname: Lü
authors.affiliation: Department of Clinical Laboratory, The First Affiliated Hospital of Bengbu Medical College, 287 Chang Huai Road, Bengbu 233004, PR China; Anhui Key Laboratory of Tissue Transplantation, Bengbu Medical College, 287 Chang Huai Road, Bengbu 233004, PR China. Electronic address: jghu9200@163.com.
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Hu
mesh_terms: D000818:Animals; D005260:Female; D009186:Myelin Sheath / Q000378:metabolism; D058953:Neural Stem Cells / Q000166:cytology*; D000073637:Oligodendrocyte Precursor Cells / Q000166:cytology* / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000502:physiology*; D012583:Schwann Cells / Q000166:cytology*; D013119:Spinal Cord Injuries / Q000503:physiopathology*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D014157:Transcription Factors
keywords: Functional recovery; Myelin gene regulatory factor; OL progenitor cells; Schwann cells; Spinal cord injury; Transplantation