title: [The analysis of major metabolic pathways in Bacillus thuringiensis under alkaline stress].
abstract: OBJECTIVE
  The study aimed to determine the appropriate stage for exploring the responseof Bacillus thuringiensis to the alkaline stress, to profile the metabolic pathways under this stress.
  
  
  METHODS
  Using semi-quantitative RT-PCR and qRT-PCR, the proper stage was defined by monitoring the transcriptional changes of marker gene pspA, which was known as a responsive gene under the alkaline stress. The total RNA was then extracted to perform the microarray hybridizations for samples under stress and control, respectively. Gene Ontology and pathway enrichments were conducted to analyze the global changes of carbon metabolism, metabolism of fatty acid synthesis and amino acid.
  
  
  RESULTS
  For B. thuringiensis in the mid-log growth phase, treatment of 28 mmol/L NaOH for 10 mins is the feasible approach to analyze the response of B. thuringiensis to this stress. More than twenty genes encoding important enzymes in glycolytic pathway were up-regulated and majority of genes involved in catalyzing alpha-ketoglutarate into malic acid were also found to up-regulated more than two folds.
  
  
  CONCLUSIONS
  By analyzing the gene expression profile, the major metabolisms of B. thuringiensis were found to be clearly enhanced under alkaline stress. Large quantities of acid including malic acid and lactic acid may contribute a lot to the adaptation of alkaline condition.
authors.forename: Guiwei
authors.initials: G
authors.lastname: Kao
authors.forename: Lili
authors.initials: L
authors.lastname: Qiu
authors.forename: Qi
authors.initials: Q
authors.lastname: Peng
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Fuping
authors.initials: F
authors.lastname: Song
mesh_terms: D000468:Alkalies / Q000378:metabolism*; D001413:Bacillus thuringiensis / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D006863:Hydrogen-Ion Concentration; D008293:Malates / Q000378:metabolism; D053858:Metabolic Networks and Pathways
chemical_list: D000468:Alkalies; D001426:Bacterial Proteins; D008293:Malates; C030298:malic acid