title: Species difference in reactivity to lignin-like enzymatically polymerized polyphenols on interferon-γ synthesis and involvement of interleukin-2 production in mice.
abstract: Recent studies have revealed that lignin-like polymerized polyphenols can activate innate immune systems. In this study, we aimed to evaluate whether these polymerized polyphenols could activate leukocytes from different murine strains. Splenocytes from 12 mouse strains were investigated. Our results revealed species differences in reactivity to phenolic polymers on interferon-γ (IFN-γ) release. Mice that possessed the H2(a) or H2(k) haplotype antigens were the highly responsive strains. To clarify these different points in soluble factors, multiplex cytokine profiling analysis was carried out and we identified interleukin (IL)-2 as a key molecule for IFN-γ induction by polymerized polyphenols. Furthermore, inhibition of IL-2 and IL-2Rα by neutralizing antibodies significantly decreased cytokine production in the highly responsive mice strains. Our results indicate that species difference in reactivity to phenolic polymers is mediated by adequate release of IL-2 and its receptor, IL-2Rα.
authors.affiliation: Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Yamanaka
authors.affiliation: Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Ishibashi
authors.affiliation: Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Adachi
authors.affiliation: Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan. Electronic address: ohnonao@toyaku.ac.jp.
authors.forename: Naohito
authors.initials: N
authors.lastname: Ohno
mesh_terms: D000818:Animals; D019138:Antibodies, Blocking / Q000494:pharmacology; D002478:Cells, Cultured; D006649:Histocompatibility Antigens / Q000235:genetics; D007113:Immunity, Innate; D007371:Interferon-gamma / Q000378:metabolism*; D007376:Interleukin-2 / Q000276:immunology / Q000378:metabolism*; D007962:Leukocytes / Q000187:drug effects* / Q000276:immunology; D008031:Lignin / Q000737:chemistry; D008213:Lymphocyte Activation / Q000187:drug effects / Q000235:genetics; D051379:Mice; D008815:Mice, Inbred Strains; D058105:Polymerization; D059808:Polyphenols / Q000737:chemistry / Q000276:immunology*; D013045:Species Specificity
chemical_list: D019138:Antibodies, Blocking; D006649:Histocompatibility Antigens; D007376:Interleukin-2; D059808:Polyphenols; D007371:Interferon-gamma; D008031:Lignin
keywords: CD25; Caffeic acid; Coumaric acid; Ferulic acid; Interferon-gamma; Interleukin-2