title: ATP phosphoribosyltransferase from symbiont Entomomyces delphacidicola invovled in histidine biosynthesis of Nilaparvata lugens (Stål).
abstract: Histidine is an essential amino acid assumed to be synthesized by an obligatory yeast-like symbiont (Entomomyces delphacidicola str. NLU) in Nilaparvata lugens, an important rice pest. The adenosine-triphosphate phosphoribosyltransferase (ATP-PRTase) facilities the committed first step of the histidine biosynthesis pathway. In the current study, a putative ATP-PRTase was cloned and verified to be of E. delphacidicola origin (EdePRTase). The expression of the gene was spatial and temporal universal with a profile that matched the distribution of the fungal symbiont. RNA interference aided the knockdown of the EdePRTase-suppressed EdePRTase expression by 32-48 %. Hemolymph histidine level was also reduced followed by significant reduction of adult body weight. However, other performance characters including nymph development, survival, and adult sex ratio were not adversely affected by the knockdown. Furthermore, forced histidine exposure (through injection or feeding) significantly inhibited the EdePRTase mRNA levels at higher concentrations, but significantly increased EdePRTase expression levels at lower concentrations (feeding only). The significance of these findings support that the EdePRTase is from symbiont E. delphacidicola, and its involvement in histidine biosynthesis of N. lugens was discussed. The results provide a better understanding of EdePRTase and the encoded functional ATP-PRTase enzyme regulation in N. lugens and insects in general.
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, China.
authors.forename: Pin-Jun
authors.initials: PJ
authors.lastname: Wan
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, China.|Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Sciences, China Jiliang University, Hangzhou, 310018, China.
authors.forename: Yao-Hua
authors.initials: YH
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, China.
authors.forename: San-Yue
authors.initials: SY
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, China.
authors.forename: Wei-Xia
authors.initials: WX
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, China.
authors.forename: Feng-Xiang
authors.initials: FX
authors.lastname: Lai
authors.affiliation: Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Sciences, China Jiliang University, Hangzhou, 310018, China. yxp@cjlu.edu.cn.
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, 310006, China. fuqiang@caas.cn.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
mesh_terms: D001276:ATP Phosphoribosyltransferase / Q000378:metabolism*; D000818:Animals; D005656:Fungal Proteins / Q000378:metabolism*; D006430:Hemiptera / Q000378:metabolism / Q000382:microbiology; D006639:Histidine / Q000096:biosynthesis*; D020037:Sordariales / Q000201:enzymology*; D013559:Symbiosis / Q000502:physiology*
chemical_list: D005656:Fungal Proteins; D006639:Histidine; D001276:ATP Phosphoribosyltransferase
keywords: ATP; Entomomyces delphacidicola; Histidine biosynthesis; Nilaparvata lugens; Phosphoribosyltransferase