title: Simultaneous Determination of Lipid Hydroperoxides by HPLC-Post Column Systems.
abstract: Two HPLC-post column systems, a conventional-size system and a semi-micro system, for the simultaneous determination of lipid hydroperoxides were developed. The hydroperoxides of free fatty acids, phosphatidylcholines, triacylglycerols and cholesterol esters were individually determined at the pmol level with good reproducibility by using gradient elution and post-column detection with diphenyl-1-pyrenylphosphine.
authors.affiliation: a Department of Applied Life Science, Graduate School of Agricultural Science, Tohoku University.
authors.forename: K
authors.initials: K
authors.lastname: Akasaka
authors.affiliation: a Department of Applied Life Science, Graduate School of Agricultural Science, Tohoku University.
authors.forename: H
authors.initials: H
authors.lastname: Ohta
authors.affiliation: a Department of Applied Life Science, Graduate School of Agricultural Science, Tohoku University.
authors.forename: Y
authors.initials: Y
authors.lastname: Hanada
authors.affiliation: a Department of Applied Life Science, Graduate School of Agricultural Science, Tohoku University.
authors.forename: H
authors.initials: H
authors.lastname: Ohrui
keywords: diphenyl-1-pyrenylphosphine; lipid hydroperoxide; post-column high-performance liquid chromatography; semi-micro high-performance liquid chromatography