title: Nanotoxicological study of polyol-made cobalt-zinc ferrite nanoparticles in rabbit.
abstract: The increasing use of engineered nanomaterials in commercial manufacturing and consumer products presents an important toxicological concern. Superparamagnetic zinc-cobalt ferrite nanoparticles (SFN) emerge as a promising tool for early cancer diagnostics and targeted therapy. However, toxicity and biological activities of SFN should be evaluated in vitro and in vivo in animal before any clinical application. In this study we aim to synthesize and characterize such objects using polyol process in order to assess its nanotoxicological profile in vitro as well as in vivo. The produced particles consist of a cobalt-zinc ferrite phase corresponding to the Zn0.8Co0.2Fe2O4 composition. They are isotropic in shape single crystals of 8nm in size. The thermal variation of their dc-magnetization confirms their superparamagnetic behavior. In vitro, acute exposure (4h) to them (100μgmL(-1)) induced an important decrease of healthy Human Umbilical Vein Endothelial Cells (HUVECs) viability. In vivo investigation in New-Zealand rabbits revealed that they lead to tissue toxicities; in lungs, liver and kidneys. Our investigations report, for the first time as far as we know, that SFN exhibit harmful properties in human cells and mammals.
authors.affiliation: Laboratoire Interface Traitement, Organisation et Dynamique des Systèmes (ITODYS), Université Paris Diderot, Sorbonne Paris Cité, CNRS UMR-7086, Paris, France; Unité de Physiologie Intégrée, Laboratoire de Pathologies Vasculaires, Faculté des Sciences de Bizerte, Université de Carthage, Jarzouna, Tunisia; Institut Cochin, Université Paris Descartes, Sorbonne Paris Cité, CNRS UMR-8104, INSERM U1016, 75005 Paris, France. Electronic address: hanini.amel@gmail.com.
authors.forename: Amel
authors.initials: A
authors.lastname: Hanini
authors.affiliation: Laboratoire de Valoristaion des Bio-Géo-Resources, Ecole Nationale de Médecine Vétérinaire de Sidi Thabet, Université de la Manouba, Sidi Thabet, Tunisia.
authors.forename: Mohamed El
authors.initials: ME
authors.lastname: Massoudi
authors.affiliation: Institut Cochin, Université Paris Descartes, Sorbonne Paris Cité, CNRS UMR-8104, INSERM U1016, 75005 Paris, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Gavard
authors.affiliation: Unité de Physiologie Intégrée, Laboratoire de Pathologies Vasculaires, Faculté des Sciences de Bizerte, Université de Carthage, Jarzouna, Tunisia.
authors.forename: Kamel
authors.initials: K
authors.lastname: Kacem
authors.affiliation: Laboratoire Interface Traitement, Organisation et Dynamique des Systèmes (ITODYS), Université Paris Diderot, Sorbonne Paris Cité, CNRS UMR-7086, Paris, France.
authors.forename: Souad
authors.initials: S
authors.lastname: Ammar
authors.affiliation: Laboratoire de Valoristaion des Bio-Géo-Resources, Ecole Nationale de Médecine Vétérinaire de Sidi Thabet, Université de la Manouba, Sidi Thabet, Tunisia.
authors.forename: Ouajdi
authors.initials: O
authors.lastname: Souilem
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D003035:Cobalt / Q000737:chemistry / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D061307:Human Umbilical Vein Endothelial Cells; D007501:Iron / Q000737:chemistry / Q000633:toxicity*; D007668:Kidney / Q000187:drug effects; D008099:Liver / Q000187:drug effects; D008168:Lung / Q000187:drug effects; D053758:Nanoparticles / Q000737:chemistry / Q000633:toxicity*; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D011108:Polymers / Q000737:chemistry*; D011817:Rabbits; D013499:Surface Properties; D018675:Toxicity Tests; D015032:Zinc / Q000737:chemistry / Q000633:toxicity*
chemical_list: D011108:Polymers; C024617:polyol; D003035:Cobalt; D007501:Iron; D015032:Zinc
keywords: Cobalt-Zinc ferrite nanoparticles; Cytotoxicity; Endothelial cell; New Zealand rabbits; Polyol process