title: Increase of β2-integrin on adhesion of THP-1 cells to collagen vitrigel membrane.
abstract: When human monocyte-derived leukemia (THP-1) cells, which are floating cells, are stimulated with lipid peroxides, or Streptococcus suis, these cells adhere to a plastic plate or endothelial cells. However, it is unclear whether or not non-stimulated THP-1 cells adhere to collagen vitrigel membrane (CVM). In this study, firstly, we investigated the rate of adhesion of THP-1 cells to CVM. When THP-1 cells were not stimulated, the rate of adhesion to CVM was high. Then, to identify adhesion molecules involved in adhesion of THP-1 cells to CVM, expressions of various cell adhesion molecules on the surface of THP-1 cells adhering to CVM were measured. β-actin, β-catenin, and β1-integrin expressions did not change in non-stimulated THP-1 cells cultured on CVM compared with those in cells cultured in a flask, but β2-integrin expression markedly increased.
authors.affiliation: a Division of Environmental Chemistry , National Institute of Health Sciences , Tokyo , Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Uchino
authors.affiliation: b Division of Pharmacology , National Institute of Health Sciences , Tokyo , Japan.
authors.forename: Yukie
authors.initials: Y
authors.lastname: Kuroda
authors.affiliation: b Division of Pharmacology , National Institute of Health Sciences , Tokyo , Japan.
authors.forename: Seiichi
authors.initials: S
authors.lastname: Ishida
authors.affiliation: c Corporate Research Center , Daicel Corporation , Himeji , Japan.
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Yamashita
authors.affiliation: c Corporate Research Center , Daicel Corporation , Himeji , Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Miyazaki
authors.affiliation: d Division of Biotechnology, Institute of Agrobiological Sciences , National Agriculture and Food Research Organization , Tsukuba , Japan.
authors.forename: Ayumi
authors.initials: A
authors.lastname: Oshikata
authors.affiliation: a Division of Environmental Chemistry , National Institute of Health Sciences , Tokyo , Japan.
authors.forename: Kumiko
authors.initials: K
authors.lastname: Shimizu
authors.affiliation: e Division of Risk Assessment , National Institute of Health Sciences , Tokyo , Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Kojima
authors.affiliation: d Division of Biotechnology, Institute of Agrobiological Sciences , National Agriculture and Food Research Organization , Tsukuba , Japan.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Takezawa
authors.affiliation: a Division of Environmental Chemistry , National Institute of Health Sciences , Tokyo , Japan.
authors.forename: Takumi
authors.initials: T
authors.lastname: Akiyama
authors.affiliation: a Division of Environmental Chemistry , National Institute of Health Sciences , Tokyo , Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Ikarashi
keywords: F-actin; THP-1; adhesion; collagen vitrigel membrane; β2-integrin