title: miR-4284 and miR-4484 as Putative Biomarkers for Diffuse Large B-Cell Lymphoma.
abstract: Diffuse large B-cell lymphoma is the most common type of non-Hodgkin lymphoma. MicroRNAs (miRNAs) are endogenous small RNA, which can regulate gene expression at the post-transcriptional level. MiRNA profiling has shown a great potential as novel diagnostic and prognostic biomarkers. The present study was performed at the Nemazee Teaching Hospital (Shiraz, Iran) from 2011 to 2013. The aim of this study was to assess the deregulation of miRNAs profiles in DLBL against hyperplasic reactive lymph node as a normal. This could serve as a biomarker for DLBL. The miRCURY LNA™ microarray was used on the total RNA, which was extracted from formalin-fixed paraffin-embedded tissue of 24 de novo diffuse large B-cell lymphoma patients and 14 normal lymph nodes. The greatest changes were detected in miR-4284 and miR-4484 level in patient's lymphoma samples. These miRNAs can act as a diagnostic biomarker for DLBL.
authors.affiliation: Department of Hematology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Gholamhossein
authors.initials: G
authors.lastname: Tamaddon
authors.affiliation: Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Bita
authors.initials: B
authors.lastname: Geramizadeh
authors.affiliation: Transplant Research Center, Nemazee Teaching Hospital, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Karimi
authors.affiliation: Department of Genetics, Faculty of Basic Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Seyed Javad
authors.initials: SJ
authors.lastname: Mowla
authors.affiliation: Department of Hematology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Said
authors.initials: S
authors.lastname: Abroun
keywords: Diffuse; Large B-Cell; Lymphoma; MicroRNAs; Microarray Analysis