title: Near-Infrared Ag2S Quantum Dots-Based DNA Logic Gate Platform for miRNA Diagnostics.
abstract: Dysregulation of miRNA expression is correlated with the development and progression of many diseases. These miRNAs are regarded as promising biomarkers. However, it is challenging to measure these low abundant molecules without employing time-consuming radioactive labeling or complex amplification strategies. Here, we present a DNA logic gate platform for miRNA diagnostics with fluorescence outputs from near-infrared (NIR) Ag2S quantum dots (QDs). Carefully designed toehold exchange-mediated strand displacements with different miRNA inputs occur on a solid-state interface, which control QDs release from solid-state interface to solution, responding to multiplex information on initial miRNAs. Excellent fluorescence emission properties of NIR Ag2S QDs certify the great prospect for amplification-free and sensitive miRNA assay. We demonstrate the potential of this platform by achieving femtomolar level miRNA analysis and the versatility of a series of logic circuits computation.
authors.affiliation: Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences , Suzhou 215163, People's Republic of China.|University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Miao
authors.affiliation: Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences , Suzhou 215163, People's Republic of China.|University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
authors.forename: Yuguo
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences , Suzhou 215163, People's Republic of China.
authors.forename: Bidou
authors.initials: B
authors.lastname: Wang
authors.affiliation: Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences , Suzhou 215163, People's Republic of China.
authors.forename: Fanyu
authors.initials: F
authors.lastname: Meng
mesh_terms: D000818:Animals; D002417:Cattle; D039301:Computers, Molecular; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D015342:DNA Probes / Q000737:chemistry / Q000235:genetics; D005453:Fluorescence; D057230:Limit of Detection; D008128:Logic; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D008856:Microscopy, Fluorescence / Q000379:methods; D057054:Molecular Imaging / Q000379:methods; D009693:Nucleic Acid Hybridization; D045663:Quantum Dots / Q000737:chemistry*; D018030:Silver Compounds / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D015342:DNA Probes; D035683:MicroRNAs; D018030:Silver Compounds; D004247:DNA; C013251:silver sulfide