title: Interaction of serologically defined colon cancer antigen-3 with Arf6 and its predominant expression in the mouse testis.
abstract: ADP ribosylation factor 6 (Arf6) is a small GTPase that regulates endosomal trafficking and actin cytoskeleton remodeling. Here, we identified the serologically defined colon antigen-3 (SDCCAG3) as an Arf6-interacting protein by yeast two-hybrid screening with a constitutively active Arf6 mutant. SDCCAG3 interacts specifically with Arf6 among the Arf family members through its 101  C-terminal amino acids. SDCCAG3 is expressed most intensely in the testis at the mRNA and protein levels. In the testis, SDCCAG3 is expressed in spermatocytes and spermatids. We also show that full-length SDCCAG3, but not a mutant lacking the ability to interact with Arf6, is recruited to the midbody during cytokinesis when expressed exogenously in HeLa cells. These findings suggest that SDCCAG3 might function in endosomal trafficking downstream of Arf6.
authors.affiliation: Department of Anatomy, Kitasato University School of Medicine, 1-15-1 Kitasato, Sagamihara, Kanagawa, 252-0374, Japan. Electronic address: sakagami@med.kitasato-u.ac.jp.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sakagami
authors.affiliation: Department of Anatomy, Kitasato University School of Medicine, 1-15-1 Kitasato, Sagamihara, Kanagawa, 252-0374, Japan.
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Hara
authors.affiliation: Department of Anatomy, Kitasato University School of Medicine, 1-15-1 Kitasato, Sagamihara, Kanagawa, 252-0374, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Fukaya
mesh_terms: D000089982:ADP-Ribosylation Factor 6; D020727:ADP-Ribosylation Factors / Q000378:metabolism*; D000818:Animals; D000951:Antigens, Neoplasm; D000954:Antigens, Surface / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D018797:Cell Cycle Proteins; D005786:Gene Expression Regulation / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009928:Organ Specificity / Q000502:physiology; D011485:Protein Binding; D013737:Testis / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D000089982:ADP-Ribosylation Factor 6; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; C000619254:SDCCAG3 protein, mouse; D020727:ADP-Ribosylation Factors; C000715727:Arf6 protein, mouse
keywords: Cytokinesis; Endosome; Membrane trafficking; Small GTPase; Testis