title: The healing effect of bone marrow-derived stem cells in acute radiation syndrome.
abstract: OBJECTIVE
  To determine the effect of bone marrow-derived mesenchymal stem cells (BMSCs) on regeneration of bone marrow and intestinal tissue and survival rate in experimental mice with acute radiation syndrome (ARS).
  
  
  METHODS
  Forty mice were randomly divided into two equal groups of A receiving no BMSC transplantation and B receiving BMSCs. BMSCs were isolated from the bone marrow and cultured in DMEM media. Both groups were irradiated with 10 Gy (dose rate 0.28 Gy/ min) (60)CO during 35 minutes with a field size of 35×35 for all the body area. Twenty-four hours after γ irradiation, 150×10(3) cells of passage 5 in 150 µl medium were injected intravenously into the tail. Animals were euthanized one and two weeks after cell transplantation. They were evaluated histologically for any changes in bone marrow and intestinal tissues. The survival rate in mice were also determined.
  
  
  RESULTS
  A significant increase for bone marrow cell count and survival rate were observed in group B in comparison to group A. Histological findings denoted to a healing in sample tissues.
  
  
  CONCLUSIONS
  BMSCs could significantly reduce the side effects of ARS and increase the survival rate and healing in injured tissue. As such their transplantation may open a window in treatment of patients with ARS.
authors.affiliation: Seyed Mohammad Javad Mortazavi, Ionizing and Non-ionizing Radiation Protection Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Seyed Mohammad Javad
authors.initials: SM
authors.lastname: Mortazavi
authors.affiliation: Fatemeh Shekoohi-Shooli , Radiology and Radiotherapy Department, Shahid Beheshti University of Medical Science, Tehran, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Shekoohi-Shooli
authors.affiliation: Seyed Mahmood Reza Aghamir, Radiology and Radiotherapy Department, Shahid Beheshti University of Medical Science, Tehran, Iran.
authors.forename: Seyed Mahmood Reza
authors.initials: SM
authors.lastname: Aghamir
authors.affiliation: Davood Mehrabani, Regenerative Medicine Department, University of Manitoba, Winnipeg, Manitoba, Canada. Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Davood
authors.initials: D
authors.lastname: Mehrabani
authors.affiliation: Amirreza Dehghanian, Trauma Research Center, Shahid Rajayee Hospital, Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Amirreza
authors.initials: A
authors.lastname: Dehghanian
authors.affiliation: Shahrokh Zare, Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Shahrokh
authors.initials: S
authors.lastname: Zare
authors.affiliation: Mohammad Amin Mosleh-Shirazi, Ionizing and Non-ionizing Radiation Protection Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Mohammad Amin
authors.initials: MA
authors.lastname: Mosleh-Shirazi
keywords: Acute radiation syndrome; Bone marrow; Healing; Mesenchymal stem cell; Mouse; Survival