title: Effects of Brinzolamide, a Topical Carbonic Anhydrase Inhibitor, on Corneal Endothelial Cells.
abstract: This clinical study aimed to investigate the effect of brinzolamide, a topical carbonic anhydrase inhibitor, on corneal endothelial cells (CECs) in patients with glaucoma using a follow-up clinical study design.
  
  
  
  Patients with primary open-angle glaucoma or ocular hypertension were administrated an ophthalmic solution of either latanoprost alone (LT) as a control (n = 18) or latanoprost plus brinzolamide (LT + BR; n = 16). CECs were examined at baseline and at 4, 12, 24, and 48 weeks in 18 and 16 eyes of the LT and LT + BR groups, respectively, using a non-contact specular microscope. CECs were evaluated by parameters, including cell density (CD), coefficient of variation (CV) in cell size, and percentage hexagonality (Hex).
  
  
  
  Compared with the baseline intraocular pressure (IOP), the mean IOP in the LT group was significantly reduced at 12 and 24 weeks, whereas that in the LT + BR group was significantly reduced at all time points (P < 0.01). The mean CD, CV, and Hex at baseline were not significantly different between the two groups. No significant time-course changes in CD, CV, or Hex were observed in either group. At 48 weeks, there was no significant difference in the mean CD, CV, or Hex between the two groups.
  
  
  
  Patients treated with LT + BR showed significant IOP reduction. However, the use of brinzolamide in addition to latanoprost had no influence on CECs during the one-year follow-up period.
authors.affiliation: Department of Ophthalmology, The Jikei University, School of Medicine, Tokyo, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Nakano
authors.affiliation: Olympia Eye Hospital, Tokyo, Japan.
authors.forename: Rishu
authors.initials: R
authors.lastname: Inoue
authors.affiliation: Ueno Ganka Clinic, Tokyo, Japan.
authors.forename: Tairo
authors.initials: T
authors.lastname: Kimura
authors.affiliation: Suzumura Ganka Clinic, Tokyo, Japan.
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Suzumura
authors.affiliation: Nishikamakura Tanino Clinic, Kanagawa, Japan.
authors.forename: Tomihiko
authors.initials: T
authors.lastname: Tanino
authors.affiliation: Department of Ophthalmology, Itabashi Hospital, Nihon University, Tokyo, Japan.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Yamazaki
authors.affiliation: Yoshikawa Eye Clinic, Tokyo, Japan.
authors.forename: Keiji
authors.initials: K
authors.lastname: Yoshikawa
authors.affiliation: Department of Preventive Medicine, Tokai University, School of Medicine, Kanagawa, Japan. tatemichi@tokai-u.jp.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Tatemichi
mesh_terms: D000368:Aged; D000959:Antihypertensive Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D002257:Carbonic Anhydrase Inhibitors / Q000008:administration & dosage / Q000627:therapeutic use*; D003315:Cornea / Q000187:drug effects*; D004359:Drug Therapy, Combination; D042783:Endothelial Cells / Q000187:drug effects*; D005260:Female; D005902:Glaucoma, Open-Angle / Q000188:drug therapy*; D006801:Humans; D007429:Intraocular Pressure / Q000187:drug effects; D000077338:Latanoprost; D008297:Male; D008875:Middle Aged; D009883:Ophthalmic Solutions; D011461:Prostaglandins F, Synthetic / Q000627:therapeutic use; D013449:Sulfonamides / Q000008:administration & dosage / Q000627:therapeutic use*; D013843:Thiazines / Q000008:administration & dosage / Q000627:therapeutic use*; D014065:Tonometry, Ocular
chemical_list: D000959:Antihypertensive Agents; D002257:Carbonic Anhydrase Inhibitors; D009883:Ophthalmic Solutions; D011461:Prostaglandins F, Synthetic; D013449:Sulfonamides; D013843:Thiazines; D000077338:Latanoprost; C111827:brinzolamide
keywords: Brinzolamide; Carbonic anhydrase inhibitor; Corneal endothelial cells; Glaucoma; Long-term effects; Ophthalmology