title: Semi Quantification of Gibberellins in the Anthers of Thermosensitive Genetic Male Sterile Rice (Oryza sativa L. cv. PL12).
abstract: The levels of endogenous GAs in the anthers of rice (Oryza sativa L., cv. Reimei (normal)) and in those of sterile and fertile plants of a thermosensitive genetic male sterile line (Norin PL12; derived from Reimei) were measured by enzyme-linked immunosorbent assay (ELISA). High levels of GA4/7 were detected in the anthers of Reimei (normal fertility) and fertile (growing under fertile conditions) PL 12, while such levels were markedly reduced with increased sterility in PL12. The anthers of the plants exposed to the sterile conditions contained undeveloped pollens. These results suggest that the occurrence of GA4/7 was closely related to the expression of the sterility gene and the growth and/or development of the anther and/or pollen PL12.
authors.affiliation: a Department of Applied Biochemistry , Utsunomiya University , 350 Mine-machi, Utsunomiya 321 , Japan.
authors.forename: I
authors.initials: I
authors.lastname: Honda
authors.affiliation: a Department of Applied Biochemistry , Utsunomiya University , 350 Mine-machi, Utsunomiya 321 , Japan.
authors.forename: S
authors.initials: S
authors.lastname: Iwasaki
authors.affiliation: a Department of Applied Biochemistry , Utsunomiya University , 350 Mine-machi, Utsunomiya 321 , Japan.
authors.forename: K
authors.initials: K
authors.lastname: Sudo
authors.affiliation: a Department of Applied Biochemistry , Utsunomiya University , 350 Mine-machi, Utsunomiya 321 , Japan.|b National Agriculture Research Center, Ministry of Agriculture , Forestry, and Fisheries , 3-1-1 Kannondai, Tsukuba 305 , Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kato
authors.affiliation: a Department of Applied Biochemistry , Utsunomiya University , 350 Mine-machi, Utsunomiya 321 , Japan.|b National Agriculture Research Center, Ministry of Agriculture , Forestry, and Fisheries , 3-1-1 Kannondai, Tsukuba 305 , Japan.
authors.forename: K
authors.initials: K
authors.lastname: Maruyama
authors.affiliation: a Department of Applied Biochemistry , Utsunomiya University , 350 Mine-machi, Utsunomiya 321 , Japan.|c Department of Applied Biological Chemistry , The University of Tokyo , 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113 , Japan.
authors.forename: M
authors.initials: M
authors.lastname: Hasegawa
authors.affiliation: a Department of Applied Biochemistry , Utsunomiya University , 350 Mine-machi, Utsunomiya 321 , Japan.|c Department of Applied Biological Chemistry , The University of Tokyo , 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113 , Japan.
authors.forename: I
authors.initials: I
authors.lastname: Yamaguchi
authors.affiliation: a Department of Applied Biochemistry , Utsunomiya University , 350 Mine-machi, Utsunomiya 321 , Japan.|c Department of Applied Biological Chemistry , The University of Tokyo , 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113 , Japan.
authors.forename: N
authors.initials: N
authors.lastname: Murofushi
authors.affiliation: a Department of Applied Biochemistry , Utsunomiya University , 350 Mine-machi, Utsunomiya 321 , Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yanagisawa
authors.affiliation: a Department of Applied Biochemistry , Utsunomiya University , 350 Mine-machi, Utsunomiya 321 , Japan.|d Frontier Research Program, The Institute of Physical and Chemical Research (RIKEN) , 2-1 Hirosawa, Wako 350-01 , Japan.
authors.forename: N
authors.initials: N
authors.lastname: Takahashi
keywords: ELISA; Oryza sativa; gibberellin; sterile; thermosensitive genetic male sterility