title: Effects of budlein A on human neutrophils and lymphocytes.
abstract: OBJECTIVE
  In this study, we evaluated whether budlein A modulates the activation of innate and adaptive immune cells such as neutrophils and lymphocytes.
  
  
  METHODS
  Our research group has investigated several plant species and several compounds have been isolated, identified, and their medical potential evaluated. Budlein A is a SL isolated from the species Aldama buddlejiformis and A. robusta (Asteraceae) and shows anti-inflammatory and anti-nociceptive activities. Advances in understanding how plant-derived substances modulate the activation of innate and adaptive immune cells have led to the development of new therapies for human diseases.
  
  
  RESULTS
  Budlein A inhibited MPO activity, IL-6, CXCL8, IL-10, and IL-12 production and induces neutrophil apoptosis. In contrast, budlein A inhibited lymphocyte proliferation and IL-2, IL-10, TGF-β, and IFN-γ production, but it did not lead to cell death.
  
  
  CONCLUSIONS
  Collectively, our results indicate that budlein A shows distinct immunomodulatory effects on immune cells.
authors.affiliation: - Universidade de São Paulo, Faculdade de Odontologia de Bauru, Departamento de Ciências Biológicas, Bauru, SP, Brasil.
authors.forename: Carollinie Dias
authors.initials: CD
authors.lastname: Knob
authors.affiliation: - Universidade de São Paulo, Faculdade de Odontologia de Bauru, Departamento de Ciências Biológicas, Bauru, SP, Brasil.
authors.forename: Milena
authors.initials: M
authors.lastname: Silva
authors.affiliation: - Universidade de São Paulo, Faculdade de Odontologia de Bauru, Departamento de Ciências Biológicas, Bauru, SP, Brasil.
authors.forename: Thaís Helena
authors.initials: TH
authors.lastname: Gasparoto
authors.affiliation: - Universidade de São Paulo, Faculdade de Odontologia de Bauru, Departamento de Ciências Biológicas, Bauru, SP, Brasil.
authors.forename: Carine Ervolino
authors.initials: CE
authors.lastname: Oliveira
authors.affiliation: - Universidade de São Paulo, Faculdade de Odontologia de Bauru, Departamento de Ciências Biológicas, Bauru, SP, Brasil.
authors.forename: Nádia Ghinelli
authors.initials: NG
authors.lastname: Amôr
authors.affiliation: - Universidade Estadual de Londrina, Londrina, PR, Brasil.
authors.forename: Nilton Syogo
authors.initials: NS
authors.lastname: Arakawa
authors.affiliation: - Universidade de São Paulo, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Departamento de Farmacognosia, Ribeirão Preto, SP, Brasil.
authors.forename: Fernando Batista
authors.initials: FB
authors.lastname: Costa
authors.affiliation: - Universidade de São Paulo, Faculdade de Odontologia de Bauru, Departamento de Ciências Biológicas, Bauru, SP, Brasil.
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Campanelli
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D019659:Asteraceae / Q000737:chemistry; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016209:Interleukin-8 / Q000032:analysis / Q000187:drug effects; D007378:Interleukins / Q000032:analysis; D007783:Lactones / Q000494:pharmacology*; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000187:drug effects*; D009195:Peroxidase / Q000032:analysis / Q000187:drug effects; D015203:Reproducibility of Results; D012717:Sesquiterpenes / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects*; D015291:Transforming Growth Factors / Q000032:analysis / Q000187:drug effects
chemical_list: D000893:Anti-Inflammatory Agents; D016209:Interleukin-8; D007378:Interleukins; D007783:Lactones; D012717:Sesquiterpenes; C520233:budlein A; D015291:Transforming Growth Factors; D009195:Peroxidase