title: Determination of the fractions of syntrophically oxidized acetate in a mesophilic methanogenic reactor through an (12)C and (13)C isotope-based kinetic model.
abstract: In order to accurately describe the carbon flow in anaerobic digestion processes, this work investigates the acetate degradation pathways through the use of stable carbon isotope analysis and a mathematical model. Batch assays using labeled (13)C acetate were employed to distinguish the acetate consumption through methanogenic Archaea and acetate-oxidizing Bacteria. Suspended and sessile biomass, with over 400 days of retention time, from a mesophilic (36.5 °C) upflow anaerobic filter was used as inocula in these assays. A three-process model for acetoclastic methanogenesis and syntrophic acetate oxidation (SAO) was developed to allow for a precise quantification of the SAO contribution. The model distinguishes carbon atoms in light and heavy isotopes, (12)C and (13)C, respectively, which permitted the simulation of the isotope ratios variation in addition to gas production, gas composition and acetate concentrations. The model indicated oxidized fractions of acetate between 7 and 18%. Due to the low free ammonia inhibition potential for the acetoclastic methanogens in these assays these findings point to the biomass retention times as a driven factor for the SAO pathway. The isotope-based kinetic model developed here also describes the δ(13)C variations in unlabeled assays accurately and has the potential to determine biological (13)C fractionation factors.
authors.affiliation: Institute of Urban Water Management and Environmental Engineering, Ruhr-Universität Bochum, Germany. Electronic address: tito.gehring@rub.de.
authors.forename: Tito
authors.initials: T
authors.lastname: Gehring
authors.affiliation: Institute of Geology, Mineralogy and Geophysics, Ruhr-Universität Bochum, Germany. Electronic address: andrea.niedermayr@rub.de.
authors.forename: Andrea
authors.initials: A
authors.lastname: Niedermayr
authors.affiliation: Institute of Urban Water Management and Environmental Engineering, Ruhr-Universität Bochum, Germany. Electronic address: stephan.berzio@rub.de.
authors.forename: Stephan
authors.initials: S
authors.lastname: Berzio
authors.affiliation: Institute of Geology, Mineralogy and Geophysics, Ruhr-Universität Bochum, Germany. Electronic address: adrian.immenhauser@rub.de.
authors.forename: Adrian
authors.initials: A
authors.lastname: Immenhauser
authors.affiliation: Institute of Urban Water Management and Environmental Engineering, Ruhr-Universität Bochum, Germany. Electronic address: marc.wichern@rub.de.
authors.forename: Marc
authors.initials: M
authors.lastname: Wichern
authors.affiliation: Institute of Urban Water Management and Environmental Engineering, Ruhr-Universität Bochum, Germany. Electronic address: Manfred.luebken@rub.de.
authors.forename: Manfred
authors.initials: M
authors.lastname: Lübken
mesh_terms: D000085:Acetates / Q000378:metabolism; D001105:Archaea / Q000378:metabolism; D019605:Euryarchaeota / Q000378:metabolism*; D007700:Kinetics; D008697:Methane / Q000378:metabolism*
chemical_list: D000085:Acetates; D008697:Methane
keywords: Anaerobic biofilm; Carbon isotope fractionation; Mathematical model; Stable carbon isotopes; Syntrophic acetate oxidation