title: Syntheses and photodynamic activity of some glucose-conjugated BODIPY dyes.
abstract: The syntheses of three water-soluble glucose-conjugated BODIPY dyes with different wavelength emissions and studies of their photodynamic therapeutic (PDT) action on human lung cancer A549 cell line are disclosed. Amongst the chosen compounds, the BODIPY dye 4 possessing a glycosylated styryl moiety at the C-3 position showed best PDT property against the A549 cell line. In particular, it induced reactive oxygen species-mediated caspase-8/caspase-3-dependent apoptosis as revealed from the increased sub G1 cell population and changes in cell morphology. These results along with its localization in the endoplasmic reticulum, as revealed by confocal microscopy suggested that mitochondria may not be directly involved in the photo-cytotoxicity of 4. Compound 4 did not induce any dark toxicity to the A549 cells, and was non-toxic to normal lung cells.
authors.affiliation: Bio-Organic Division, Bhabha Atomic Resesarch Centre, Mumbai 400 085, India.
authors.forename: Neelam
authors.initials: N
authors.lastname: Shivran
authors.affiliation: Bio-Organic Division, Bhabha Atomic Resesarch Centre, Mumbai 400 085, India.
authors.forename: Mrityunjay
authors.initials: M
authors.lastname: Tyagi
authors.affiliation: Bio-Organic Division, Bhabha Atomic Resesarch Centre, Mumbai 400 085, India.
authors.forename: Soumyaditya
authors.initials: S
authors.lastname: Mula
authors.affiliation: Bio-Organic Division, Bhabha Atomic Resesarch Centre, Mumbai 400 085, India.
authors.forename: Pooja
authors.initials: P
authors.lastname: Gupta
authors.affiliation: Bio-Organic Division, Bhabha Atomic Resesarch Centre, Mumbai 400 085, India.
authors.forename: Bhaskar
authors.initials: B
authors.lastname: Saha
authors.affiliation: Bio-Organic Division, Bhabha Atomic Resesarch Centre, Mumbai 400 085, India.
authors.forename: Birija S
authors.initials: BS
authors.lastname: Patro
authors.affiliation: Bio-Organic Division, Bhabha Atomic Resesarch Centre, Mumbai 400 085, India. Electronic address: schatt@barc.gov.in.
authors.forename: Subrata
authors.initials: S
authors.lastname: Chattopadhyay
mesh_terms: D001692:Biological Transport; D001896:Boron Compounds / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D045744:Cell Line, Tumor; D060326:Chemistry Techniques, Synthetic; D004396:Coloring Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D005947:Glucose / Q000737:chemistry*; D006801:Humans; D010778:Photochemotherapy
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D004396:Coloring Agents; D005947:Glucose
keywords: Apoptosis; BODIPY dyes; Lung cancer; Photodynamic property