title: Visfatin expression analysis in association with recruitment and activation of human and rodent brown and brite adipocytes.
abstract: Human brown adipocytes are able to burn fat and glucose and are now considered as a potential strategy to treat obesity, type 2 diabetes and metabolic disorders. Besides their thermogenic function, brown adipocytes are able to secrete adipokines. One of these is visfatin, a nicotinamide phosphoribosyltransferase involved in nicotinamide dinucleotide synthesis, which is known to participate in the synthesis of insulin by pancreatic β cells. In a therapeutic context, it is of interest to establish whether a potential correlation exists between brown adipocyte activation and/or brite adipocyte recruitment, and adipokine expression. We analyzed visfatin expression, as a pre-requisite to its secretion, in rodent and human biopsies and cell models of brown/brite adipocytes. We found that visfatin was preferentially expressed in mature adipocytes and that this expression was higher in brown adipose tissue of rodents compared to other fat depots. However, using various rodent models we were unable to find any correlation between visfatin expression and brown or brite adipocyte activation or recruitment. Interestingly, the situation is different in humans where visfatin expression was found to be equivalent between white and brown or brite adipocytes in vivo and in vitro. In conclusion, visfatin can be considered only as a rodent brown adipocyte biomarker, independently of tissue activation.
authors.affiliation: Univ. Nice-Sophia Antipolis, UFR Medecine, Nice, France; CNRS, iBV, UMR, 7277, Nice, France; INSERM, iBV, U1091, Nice, France.
authors.forename: Didier F
authors.initials: DF
authors.lastname: Pisani
authors.affiliation: Univ. of Nice-Sophia Antipolis, Institute for Research on Cancer and Aging of Nice (IRCAN), Nice, France; INSERM, IRCAN, U1081, Nice, France; CNRS, IRCAN, UMR7284, Nice, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Dumortier
authors.affiliation: Univ. Nice-Sophia Antipolis, UFR Medecine, Nice, France; CNRS, iBV, UMR, 7277, Nice, France; INSERM, iBV, U1091, Nice, France.
authors.forename: Guillaume E
authors.initials: GE
authors.lastname: Beranger
authors.affiliation: Univ. of Nice-Sophia Antipolis, Institute for Research on Cancer and Aging of Nice (IRCAN), Nice, France; INSERM, IRCAN, U1081, Nice, France; CNRS, IRCAN, UMR7284, Nice, France.
authors.forename: Virginie
authors.initials: V
authors.lastname: Casamento
authors.affiliation: Univ. Nice-Sophia Antipolis, UFR Medecine, Nice, France; CNRS, iBV, UMR, 7277, Nice, France; INSERM, iBV, U1091, Nice, France.
authors.forename: Rayane A
authors.initials: RA
authors.lastname: Ghandour
authors.affiliation: Univ. Nice-Sophia Antipolis, UFR Medecine, Nice, France; CNRS, iBV, UMR, 7277, Nice, France; INSERM, iBV, U1091, Nice, France.
authors.forename: Maude
authors.initials: M
authors.lastname: Giroud
authors.affiliation: Univ. of Nice-Sophia Antipolis, Institute for Research on Cancer and Aging of Nice (IRCAN), Nice, France; INSERM, IRCAN, U1081, Nice, France; CNRS, IRCAN, UMR7284, Nice, France.
authors.forename: Nadine
authors.initials: N
authors.lastname: Gautier
authors.affiliation: Univ. Nice-Sophia Antipolis, UFR Medecine, Nice, France; CNRS, iBV, UMR, 7277, Nice, France; INSERM, iBV, U1091, Nice, France; Centre Hospitalo-Universitaire Nice, Department of Plastic, Reconstructive and Hand Surgery, St-Roch Hospital, Nice, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Balaguer
authors.affiliation: Univ. Nice-Sophia Antipolis, UFR Medecine, Nice, France; CNRS, iBV, UMR, 7277, Nice, France; INSERM, iBV, U1091, Nice, France.
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Chambard
authors.affiliation: Turku PET Center, University of Turku, Turku, Finland; Turku PET Center, Turku University Hospital, Turku, Finland.
authors.forename: Kirsi A
authors.initials: KA
authors.lastname: Virtanen
authors.affiliation: Turku PET Center, University of Turku, Turku, Finland; Department of Endocrinology, Turku University Hospital, Turku, Finland.
authors.forename: Pirjo
authors.initials: P
authors.lastname: Nuutila
authors.affiliation: Department of Surgery, Turku University Hospital , Turku, Finland.
authors.forename: Tarja
authors.initials: T
authors.lastname: Niemi
authors.affiliation: Department of Anesthesiology, Turku University Hospital , Turku, Finland.
authors.forename: Markku
authors.initials: M
authors.lastname: Taittonen
authors.affiliation: Univ. of Nice-Sophia Antipolis, Institute for Research on Cancer and Aging of Nice (IRCAN), Nice, France; INSERM, IRCAN, U1081, Nice, France; CNRS, IRCAN, UMR7284, Nice, France; Centre Hospitalo-Universitaire Nice, Biochemistry Laboratory, Pasteur Hospital, Nice, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Van Obberghen
authors.affiliation: Univ. of Nice-Sophia Antipolis, Institute for Research on Cancer and Aging of Nice (IRCAN), Nice, France; INSERM, IRCAN, U1081, Nice, France; CNRS, IRCAN, UMR7284, Nice, France; Centre Hospitalo-Universitaire Nice, Biochemistry Laboratory, Pasteur Hospital, Nice, France.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Hinault
authors.affiliation: Univ. Nice-Sophia Antipolis, UFR Medecine, Nice, France; CNRS, iBV, UMR, 7277, Nice, France; INSERM, iBV, U1091, Nice, France.
authors.forename: Ez-Zoubir
authors.initials: EZ
authors.lastname: Amri
keywords: PBEF; UCP1; hMADS; nampt; obesity; type 2 diabetes