title: Autophagy Gene Activity May Act As a Key Factor for Sensitivity of Tumor Cells to Oncolytic Vesicular Stomatitis Virus.
abstract: BACKGROUND
  Beclin1 is an important, primary molecule for autophagy.
  
  
  OBJECTIVE
  It is suggested that the control of the autophagy path increases the sensitivity of tumor cells to VSV.
  
  
  METHODS
  In this study, the degree of Beclin1 gene expression in two cell lines, HeLa and A549, has been examined and the percentage of living cells subsequent infection with virus has been evaluated by MTT assay method.
  
  
  RESULTS
  The results showed that the degree of Beclin1 gene expression in HeLa cells in comparison with A549 cells has reduced, and the sensitivity of these cells to vesicular stomatits virus (VSV) oncolysis is more than A549.
  
  
  CONCLUSIONS
  It seems that by using some methods for reducing autophagy, it is possible to make tumor cells more sensitive to virotherapy and even other treatments.
authors.affiliation: Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IR Iran.
authors.forename: Arezoo
authors.initials: A
authors.lastname: Nabizadeh
authors.affiliation: Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IR Iran.
authors.forename: Taravat
authors.initials: T
authors.lastname: Bamdad
authors.affiliation: Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, IR Iran.
authors.forename: Ehsan
authors.initials: E
authors.lastname: Arefian
authors.affiliation: Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IR Iran; Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, IR Iran.
authors.forename: Seyed Hadi
authors.initials: SH
authors.lastname: Razavi Nikoo
keywords: Autophagy; Beclin1; Vesicular Stomatitis Virus; Virotherapy