title: A new protoparvovirus in human fecal samples and cutaneous T cell lymphomas (mycosis fungoides).
abstract: We genetically characterized seven nearly complete genomes in the protoparvovirus genus from the feces of children with diarrhea. The viruses, provisionally named cutaviruses (CutaV), varied by 1-6% nucleotides and shared ~76% and ~82% amino acid identity with the NS1 and VP1 of human bufaviruses, their closest relatives. Using PCR, cutavirus DNA was found in 1.6% (4/245) and 1% (1/100) of diarrhea samples from Brazil and Botswana respectively. In silico analysis of pre-existing metagenomics datasets then revealed closely related parvovirus genomes in skin biopsies from patients with epidermotropic cutaneous T-cell lymphoma (CTCL or mycosis fungoides). PCR of skin biopsies yielded cutavirus DNA in 4/17 CTCL, 0/10 skin carcinoma, and 0/21 normal or noncancerous skin biopsies. In situ hybridization of CTCL skin biopsies detected viral genome within rare individual cells in regions of neoplastic infiltrations. The influence of cutavirus infection on human enteric functions and possible oncolytic role in CTCL progression remain to be determined.
authors.affiliation: Blood Systems Research Institute, San Francisco, CA 94118, USA; Department of Laboratory Medicine, University of California at San Francisco, San Francisco, CA, USA.
authors.forename: Tung G
authors.initials: TG
authors.lastname: Phan
authors.affiliation: Department of Dermatology, Nantes University Hospital, INSERM U 892, Nantes, France. Electronic address: brigitte.dreno@wanadoo.fr.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Dreno
authors.affiliation: Blood Systems Research Institute, San Francisco, CA 94118, USA; Institute of Tropical Medicine, School of Medicine, University of São Paulo, São Paulo, Brazil.
authors.forename: Antonio Charlys
authors.initials: AC
authors.lastname: da Costa
authors.affiliation: Blood Systems Research Institute, San Francisco, CA 94118, USA; Department of Laboratory Medicine, University of California at San Francisco, San Francisco, CA, USA.
authors.forename: Linlin
authors.initials: L
authors.lastname: Li
authors.affiliation: Oswaldo Cruz Foundation, Manaus, Brazil.
authors.forename: Patricia
authors.initials: P
authors.lastname: Orlandi
authors.affiliation: Blood Systems Research Institute, San Francisco, CA 94118, USA; Department of Laboratory Medicine, University of California at San Francisco, San Francisco, CA, USA.
authors.forename: Xutao
authors.initials: X
authors.lastname: Deng
authors.affiliation: Blood Systems Research Institute, San Francisco, CA 94118, USA; Department of Laboratory Medicine, University of California at San Francisco, San Francisco, CA, USA.
authors.forename: Beatrix
authors.initials: B
authors.lastname: Kapusinszky
authors.affiliation: Blood Systems Research Institute, San Francisco, CA 94118, USA; Programa de Oncovirologia, Instituto Nacional de Câncer, Rio de Janeiro, Brazil.
authors.forename: Juliana
authors.initials: J
authors.lastname: Siqueira
authors.affiliation: Department of Dermatology, Nantes University Hospital, INSERM U 892, Nantes, France.
authors.forename: Anne-Chantal
authors.initials: AC
authors.lastname: Knol
authors.affiliation: Institute for Transplantation/Urology and Nephrology, Nantes University Hospital, Nantes F44093, France; INSERM, UMR 1064-Center for Research in Transplantation and Immunology, Nantes F44093, France; University of Nantes, Nantes F44093, France.
authors.forename: Franck
authors.initials: F
authors.lastname: Halary
authors.affiliation: Department of Nephrology, Clinical Immunology, Transplantation, Nantes University Hospital, Nantes, France.
authors.forename: Jacques
authors.initials: J
authors.lastname: Dantal
authors.affiliation: Department of Fisheries and Wildlife Conservation, Virginia Tech, Blacksburg, VA, USA; CARACAL, Centre for African Resources: Animals, Communities, and Land Use, Kasane, Botswana.
authors.forename: Kathleen A
authors.initials: KA
authors.lastname: Alexander
authors.affiliation: Department of Pathology, Microbiology and Immunology, University of California, Davis, CA, USA.
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Pesavento
authors.affiliation: Blood Systems Research Institute, San Francisco, CA 94118, USA; Department of Laboratory Medicine, University of California at San Francisco, San Francisco, CA, USA. Electronic address: delwarte@medicine.ucsf.edu.
authors.forename: Eric
authors.initials: E
authors.lastname: Delwart
mesh_terms: D001706:Biopsy; D004279:DNA, Viral; D005243:Feces / Q000821:virology*; D006801:Humans; D054892:Metagenome; D056186:Metagenomics; D009182:Mycosis Fungoides / Q000209:etiology* / Q000473:pathology; D016366:Open Reading Frames; D010322:Parvoviridae Infections / Q000150:complications / Q000821:virology*; D017992:Parvovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D012326:RNA Splicing
chemical_list: D004279:DNA, Viral
keywords: CTCL; Cutavirus; Oncolytic; Protoparvovirus