title: Multimodal Mass Spectrometry Imaging of N-Glycans and Proteins from the Same Tissue Section.
abstract: On-tissue digestion matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) can be used to record spatially correlated molecular information from formalin-fixed, paraffin-embedded (FFPE) tissue sections. In this work, we present the in situ multimodal analysis of N-linked glycans and proteins from the same FFPE tissue section. The robustness and applicability of the method are demonstrated for several tumors, including epithelial and mesenchymal tumor types. Major analytical aspects, such as lateral diffusion of the analyte molecules and differences in measurement sensitivity due to the additional sample preparation methods, have been investigated for both N-glycans and proteolytic peptides. By combining the MSI approach with extract analysis, we were also able to assess which mass spectral peaks generated by MALDI-MSI could be assigned to unique N-glycan and peptide identities.
authors.affiliation: Center for Proteomics and Metabolomics, Leiden University Medical Center , Leiden, The Netherlands.
authors.forename: Bram
authors.initials: B
authors.lastname: Heijs
authors.affiliation: Center for Proteomics and Metabolomics, Leiden University Medical Center , Leiden, The Netherlands.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Holst
authors.affiliation: Department of Pathology, Leiden University Medical Center , Leiden, The Netherlands.
authors.forename: Inge H
authors.initials: IH
authors.lastname: Briaire-de Bruijn
authors.affiliation: Department of Surgery, Leiden University Medical Center , Leiden, The Netherlands.
authors.forename: Gabi W
authors.initials: GW
authors.lastname: van Pelt
authors.affiliation: Center for Proteomics and Metabolomics, Leiden University Medical Center , Leiden, The Netherlands.
authors.forename: Arnoud H
authors.initials: AH
authors.lastname: de Ru
authors.affiliation: Center for Proteomics and Metabolomics, Leiden University Medical Center , Leiden, The Netherlands.
authors.forename: Peter A
authors.initials: PA
authors.lastname: van Veelen
authors.affiliation: Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina , Charleston, South Carolina 29425, United States.
authors.forename: Richard R
authors.initials: RR
authors.lastname: Drake
authors.affiliation: Department of Microbiology and Immunology, College of Medicine, Drexel University , Philadelphia, Pennsylvania 19129, United States.
authors.forename: Anand S
authors.initials: AS
authors.lastname: Mehta
authors.affiliation: Department of Surgery, Leiden University Medical Center , Leiden, The Netherlands.
authors.forename: Wilma E
authors.initials: WE
authors.lastname: Mesker
authors.affiliation: Department of Surgery, Leiden University Medical Center , Leiden, The Netherlands.
authors.forename: Rob A
authors.initials: RA
authors.lastname: Tollenaar
authors.affiliation: Department of Pathology, Leiden University Medical Center , Leiden, The Netherlands.
authors.forename: Judith V M G
authors.initials: JV
authors.lastname: Bovée
authors.affiliation: Center for Proteomics and Metabolomics, Leiden University Medical Center , Leiden, The Netherlands.
authors.forename: Manfred
authors.initials: M
authors.lastname: Wuhrer
authors.affiliation: Center for Proteomics and Metabolomics, Leiden University Medical Center , Leiden, The Netherlands.|Department of Pathology, Leiden University Medical Center , Leiden, The Netherlands.|Fondazione Pisana per la Scienza ONLUS , Pisa, Italy.
authors.forename: Liam A
authors.initials: LA
authors.lastname: McDonnell
mesh_terms: D000941:Antigens / Q000378:metabolism; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000473:pathology; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D007890:Leiomyosarcoma / Q000378:metabolism / Q000473:pathology; D018208:Liposarcoma, Myxoid / Q000378:metabolism / Q000473:pathology; D016612:Paraffin Embedding; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / Q000378:metabolism; D010455:Peptides / Q000032:analysis* / Q000378:metabolism; D011134:Polysaccharides / Q000032:analysis* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000941:Antigens; D006023:Glycoproteins; D010455:Peptides; D011134:Polysaccharides; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase