title: Effects of Fibronectin Coating on Bacterial and Osteoblast Progenitor Cells Adherence in a Co-culture Assay.
abstract: Bacterial adherence to the surface of implants functionalized with cell-adhesive biomolecules is a critical first step of infection development. This study was designed to determine how the immobilization of human plasmatic fibronectin (pFN) could impact bacterial and osteoblast cells interaction with the surface during concomitant exposition to the two cell-types. Calibrated suspensions of P. aeruginosa PAOI or S. aureus CIP4.83 bacteria and STRO-1+A osteoblast progenitor cells were mixed, co-seeded on glass coverslips coated or not with pFN and incubated at 37 °C. After 3 h of co-culture, the presence of bacteria did not modify the STRO-1+A cells adherence to glass. pFN coating significantly enhanced STRO-1+A cells, CIP4.83 and PAOI adherence to glass and bacterial interaction with STRO-1+A cells. Confocal laser scanning microscopy observations revealed that cells on the pFN-coated substrate exhibited a greater spreading, better organized network of cytoskeletal filaments, and an increased cellular FN expression than cells on the uncoated substrate. The use of fluorescently labeled pFN showed that adherent STRO-1+A cells were able to remodel and to concentrate coated pFN at the cells surface. Thus, the use of FN coating could increase the risk of bacterial adherence to the material surface, acting either directly onto the coating layer or indirectly on adherent osteoblastic cells. This may increase the infection risk in the presence of bacterial contamination.
authors.affiliation: Laboratoire ERRMECe-EA1391, Université de Cergy-Pontoise, 2 avenue Adolphe Chauvin, BP222 - 95302, Cergy-Pontoise, France.
authors.forename: Mathilde
authors.initials: M
authors.lastname: Hindié
authors.affiliation: Laboratoire ERRMECe-EA1391, Université de Cergy-Pontoise, 2 avenue Adolphe Chauvin, BP222 - 95302, Cergy-Pontoise, France.
authors.forename: Dongni
authors.initials: D
authors.lastname: Wu
authors.affiliation: Institut de Science des Matériaux de Mulhouse (IS2M) UMR CNRS 7361, Université de Haute-Alsace, 15 rue Jean Starcky, BP 2488-68057, Mulhouse, France.
authors.forename: Karine
authors.initials: K
authors.lastname: Anselme
authors.affiliation: Laboratoire ERRMECe-EA1391, Université de Cergy-Pontoise, 2 avenue Adolphe Chauvin, BP222 - 95302, Cergy-Pontoise, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Gallet
authors.affiliation: Laboratoire ERRMECe-EA1391, Université de Cergy-Pontoise, 2 avenue Adolphe Chauvin, BP222 - 95302, Cergy-Pontoise, France. Patrick.di-martino@u-cergy.fr.
authors.forename: Patrick
authors.initials: P
authors.lastname: Di Martino
mesh_terms: D000954:Antigens, Surface; D001422:Bacterial Adhesion; D002448:Cell Adhesion; D002478:Cells, Cultured; D018920:Coculture Techniques; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000502:physiology*; D013211:Staphylococcus aureus / Q000254:growth & development / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D000954:Antigens, Surface; C067625:FN1 protein, human; D005353:Fibronectins; C525591:STRO-1 antigen, human
keywords: Adherence; Fibronectin; Osteoblast; Pseudomonas aeruginosa; Staphylococcus aureus