title: Liver proteome of mice with different genetic susceptibilities to the effects of fluoride.
abstract: OBJECTIVE
  In this study, we investigated the differential pattern of protein expression in the liver of these mice to provide insights on why they have different responses to F.
  
  
  METHODS
  Weanling male A/J and 129P3/J mice (n=10 from each strain) were pared and housed in metabolic cages with ad libitum access to low-F food and deionized water for 42 days. Liver proteome profiles were examined using nLC-MS/MS. Protein function was classified by GO biological process (Cluego v2.0.7 + Clupedia v1.0.8) and protein-protein interaction network was constructed (PSICQUIC, Cytoscape).
  
  
  RESULTS
  Most proteins with fold change were increased in A/J mice. The functional category with the highest percentage of altered genes was oxidation-reduction process (20%). Subnetwork analysis revealed that proteins with fold change interacted with Disks large homolog 4 and Calcium-activated potassium channel subunit alpha-1. A/J mice had an increase in proteins related to energy flux and oxidative stress.
  
  
  CONCLUSIONS
  This could be a possible explanation for the high susceptibility of these mice to the effects of F, since the exposure also induces oxidative stress.
authors.affiliation: - Universidade de São Paulo, Faculdade de Odontologia de Bauru, Departamento de Ciências Biológicas, Bauru, SP, Brasil.
authors.forename: Zohaib Nisar
authors.initials: ZN
authors.lastname: Khan
authors.affiliation: - Universidade de São Paulo, Faculdade de Odontologia de Bauru, Departamento de Ciências Biológicas, Bauru, SP, Brasil.|- Universidade Federal de São Carlos, Centro de Ciências Biológicas e da Saúde, Departamento de Genética e Evolução, São Carlos, SP, Brasil.
authors.forename: Aline de Lima
authors.initials: Ade L
authors.lastname: Leite
authors.affiliation: - Universidade de São Paulo, Faculdade de Odontologia de Bauru, Departamento de Ciências Biológicas, Bauru, SP, Brasil.
authors.forename: Senda
authors.initials: S
authors.lastname: Charone
authors.affiliation: - Universidade de São Paulo, Faculdade de Odontologia de Bauru, Departamento de Ciências Biológicas, Bauru, SP, Brasil.
authors.forename: Isabela Tomazini
authors.initials: IT
authors.lastname: Sabino
authors.affiliation: - Universidade de São Paulo, Faculdade de Odontologia de Bauru, Departamento de Ciências Biológicas, Bauru, SP, Brasil.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Martini
authors.affiliation: - Universidade Federal de São Carlos, Centro de Ciências Biológicas e da Saúde, Departamento de Genética e Evolução, São Carlos, SP, Brasil.
authors.forename: Heloísa Aparecida Barbosa da Silva
authors.initials: HA
authors.lastname: Pereira
authors.affiliation: - Universidade de São Paulo, Faculdade de Odontologia de Bauru, Departamento de Ciências Biológicas, Bauru, SP, Brasil.
authors.forename: Rodrigo Cardoso
authors.initials: RC
authors.lastname: Oliveira
authors.affiliation: - Universidade de São Paulo, Faculdade de Odontologia de Bauru, Departamento de Ciências Biológicas, Bauru, SP, Brasil.
authors.forename: Marília Afonso Rabelo
authors.initials: MA
authors.lastname: Buzalaf
mesh_terms: D000818:Animals; D005459:Fluorides / Q000032:analysis / Q000378:metabolism / Q000633:toxicity*; D009050:Fluorosis, Dental / Q000235:genetics*; D015870:Gene Expression; D020022:Genetic Predisposition to Disease; D008099:Liver / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D051379:Mice; D057507:Mice, 129 Strain; D008805:Mice, Inbred A; D018384:Oxidative Stress / Q000187:drug effects; D054730:Protein Interaction Domains and Motifs; D011506:Proteins / Q000032:analysis* / Q000187:drug effects / Q000235:genetics; D020543:Proteome / Q000187:drug effects*; D040901:Proteomics / Q000379:methods; D012016:Reference Values; D013997:Time Factors
chemical_list: D011506:Proteins; D020543:Proteome; D005459:Fluorides