title: Proposal of Helicobacter canicola sp. nov., previously identified as Helicobacter cinaedi, isolated from canines.
abstract: During the course of our taxonomic investigation of Helicobacter cinaedi, it was realized that the strains isolated from dogs, which have been identified as H. cinaedi, showed different biochemical traits than did the isolates obtained from humans. None of the three dog isolates could reduce nitrate to nitrite, whereas all of the human H. cinaedi isolates could do so. The dog isolates showed a strong positive alkaline phosphatase reaction and could grow at 42°C, however the human isolates showed negative to very weak responses to those tests. The GyrA protein based phylogenetic analysis showed that the three isolates from dogs formed a slightly distinct cluster from the human isolate cluster. Phylogenetic analysis of the 16S rRNA, 23S rRNA, gyrB, and hsp60 gene sequences further confirmed that the dog isolates differed from the human H. cinaedi isolate cluster. The whole-genome in silico DNA similarities of each isolate based on their full genome sequences revealed that the isolates from dogs shared more than 94.9% ANIb (average nucleotide identity based on BLAST), while 94.0% ANIb were found between the isolates from dogs and the humans, including the H. cinaedi type strain ATCC BAA-847(T) (=CCUG 18818(T)). From these data, we propose a new species, 'H. canicola' sp. nov., for the isolates from dogs. The type strain is PAGU 1410(T) (CCUG 33887(T)=LMG 29580(T)).
authors.affiliation: Department of Microbiology, Aichi Gakuin University, School of Pharmacy, 1-100 Kusumoto-cho, Nagoya, Aichi 464-8650, Japan. Electronic address: kawamura@dpc.agu.ac.jp.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kawamura
authors.affiliation: Department of Microbiology, Aichi Gakuin University, School of Pharmacy, 1-100 Kusumoto-cho, Nagoya, Aichi 464-8650, Japan. Electronic address: jtomida@dpc.agu.ac.jp.
authors.forename: Junko
authors.initials: J
authors.lastname: Tomida
authors.affiliation: Pathogenic Microbe Laboratory, Research Institute, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjuku, Tokyo 162-8655, Japan. Electronic address: takiyam@ri.ncgm.go.jp.
authors.forename: Tohru
authors.initials: T
authors.lastname: Miyoshi-Akiyama
authors.affiliation: Intensive Care Unit, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjuku, Tokyo 162-8655, Japan. Electronic address: tatton.ncgm@gmail.com.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Okamoto
authors.affiliation: Division of Infectious Diseases, Department of Medicine, Okinawa Chubu Hospital, 281 Miyazato, Uruma, Okinawa 902-2293, Japan. Electronic address: masashi.narita@gmail.com.
authors.forename: Masashi
authors.initials: M
authors.lastname: Narita
authors.affiliation: Emergency, Critical Care and Anesthesia, Ohta General Hospital Foundation, Ohta Nishinouchi Hospital, 2-5-20, Nishinouchi, Koriyama, Fukushima 963-8558, Japan. Electronic address: katsu0419@gmail.com.
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Hashimoto
authors.affiliation: Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium. Electronic address: Margo.Cnockaert@UGent.be.
authors.forename: Margo
authors.initials: M
authors.lastname: Cnockaert
authors.affiliation: Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium. Electronic address: Peter.Vandamme@UGent.be.
authors.forename: Peter
authors.initials: P
authors.lastname: Vandamme
authors.affiliation: Department of Microbiology, Aichi Gakuin University, School of Pharmacy, 1-100 Kusumoto-cho, Nagoya, Aichi 464-8650, Japan. Electronic address: yujmor@dpc.agu.ac.jp.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Morita
authors.affiliation: Department of Microbiology, Graduate School of Medical Sciences, Kumamoto University, 1-1-1 Honjou, Kumamoto, Kumamoto 860-8556, Japan. Electronic address: sawat@kumamoto-u.ac.jp.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Sawa
authors.affiliation: Department of Environmental Health Sciences and Molecular Toxicology, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba, Sendai, Miyagi 980-8575, Japan. Electronic address: takaike@med.tohoku.ac.jp.
authors.forename: Takaaki
authors.initials: T
authors.lastname: Akaike
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics; D000818:Animals; D018834:Chaperonin 60 / Q000235:genetics*; D027081:DNA Gyrase / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004285:Dogs / Q000382:microbiology*; D016680:Genome, Bacterial / Q000235:genetics; D016998:Helicobacter / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D058889:Molecular Typing / Q000662:veterinary*; D009566:Nitrates / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012338:RNA, Ribosomal, 23S / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D018834:Chaperonin 60; D004269:DNA, Bacterial; D009566:Nitrates; D009573:Nitrites; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D000469:Alkaline Phosphatase; D027081:DNA Gyrase
keywords: 16S rRNA; 23S rRNA; Average nucleotide identity (ANI); Canine; DNA gyrase; DNA–DNA hybridization; Heat shock protein; In silico DNA similarity analysis