title: Accelerated follicle growth during the culture of isolated caprine preantral follicles is detrimental to follicular survival and oocyte meiotic resumption.
abstract: This study investigated the effect of androstenedione (A4) alone or in association with different concentrations of bovine recombinant FSH on the IVC of isolated goat preantral follicles. Follicles were mechanically isolated from ovarian tissue and cultured for 18 days in α-minimum essential medium supplemented or not with A4 (10 ng/mL) alone or in association with fixed (A4 + FixFSH: 100 ng/mL) or sequential (A4 + SeqFSH: Day 0, 100 ng/mL; Day 6, 500 ng/mL; Day 12, 1000 ng/mL) concentrations of FSH. After 18 days, the oocytes were recovered for IVM and fluorescence analysis. At Day 18 of culture, only A4 + SeqFSH treatment showed a lower (P < 0.05) rate of intact follicles, survival probability, and meiotic resumption, as well as higher (P < 0.05) percentage of degeneration and/or extrusion after antrum formation. Taken together, these results reported a positive correlation between fast-growing follicles and follicles that degenerated and/or extruded after antrum formation. When compared with control, the addition of A4 alone or in association of FSH did not increase (P > 0.05) the estradiol production or androstenedione levels on Day 6. However, on Day 18, the androstenedione levels were significantly lower in A4 + SeqFSH treatment when compared with A4 alone or to A4 + FixFSH treatments, whereas the estradiol production did not differ (P > 0.05). In summary, this study found that accelerated follicle growth negatively impacted the morphology of caprine preantral follicle cultured in vitro. In addition, the association of androstenedione with increasing concentration of FSH was detrimental to follicular survival and oocyte meiotic resumption.
authors.affiliation: Laboratory of Manipulation of Oocyte Enclosed in Preantral Follicles-LAMOFOPA, Faculty of Veterinary, State University of Ceará, Fortaleza, Brazil.
authors.forename: Livia Brunetti
authors.initials: LB
authors.lastname: Apolloni
authors.affiliation: Laboratory of Manipulation of Oocyte Enclosed in Preantral Follicles-LAMOFOPA, Faculty of Veterinary, State University of Ceará, Fortaleza, Brazil.
authors.forename: Jamily Bezerra
authors.initials: JB
authors.lastname: Bruno
authors.affiliation: Laboratory of Manipulation of Oocyte Enclosed in Preantral Follicles-LAMOFOPA, Faculty of Veterinary, State University of Ceará, Fortaleza, Brazil.
authors.forename: Benner Geraldo
authors.initials: BG
authors.lastname: Alves
authors.affiliation: Laboratory of Manipulation of Oocyte Enclosed in Preantral Follicles-LAMOFOPA, Faculty of Veterinary, State University of Ceará, Fortaleza, Brazil.
authors.forename: Anna Clara Accioly
authors.initials: ACA
authors.lastname: Ferreira
authors.affiliation: Laboratory of Manipulation of Oocyte Enclosed in Preantral Follicles-LAMOFOPA, Faculty of Veterinary, State University of Ceará, Fortaleza, Brazil.
authors.forename: Victor Macêdo
authors.initials: VM
authors.lastname: Paes
authors.affiliation: Laboratory of Manipulation of Oocyte Enclosed in Preantral Follicles-LAMOFOPA, Faculty of Veterinary, State University of Ceará, Fortaleza, Brazil.
authors.forename: Jesus de Los Reyes Cadenas
authors.initials: JLRC
authors.lastname: Moreno
authors.affiliation: Laboratory of Manipulation of Oocyte Enclosed in Preantral Follicles-LAMOFOPA, Faculty of Veterinary, State University of Ceará, Fortaleza, Brazil.
authors.forename: Francisco Léo Nascimento
authors.initials: FLN
authors.lastname: de Aguiar
authors.affiliation: Department of Animal Reproduction, Faculty of Veterinary, Federal University Fluminense, Niterói, Rio de Janeiro, Brazil.
authors.forename: Felipe Zandonadi
authors.initials: FZ
authors.lastname: Brandão
authors.affiliation: Follicles Biology Laboratory, Center for Reproductive Medicine, UZ Brussel, Brussels, Belgium.
authors.forename: Johan
authors.initials: J
authors.lastname: Smitz
authors.affiliation: Department of Animal Science, Food and Nutrition, Southern Illinois University, Carbondale, USA.
authors.forename: Gary
authors.initials: G
authors.lastname: Apgar
authors.affiliation: Laboratory of Manipulation of Oocyte Enclosed in Preantral Follicles-LAMOFOPA, Faculty of Veterinary, State University of Ceará, Fortaleza, Brazil. Electronic address: jrf.lamofopapapers@gmail.com.
authors.forename: José Ricardo
authors.initials: JR
authors.lastname: de Figueiredo
mesh_terms: D000735:Androstenedione / Q000096:biosynthesis / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D003470:Culture Media; D004958:Estradiol / Q000096:biosynthesis; D005260:Female; D005640:Follicle Stimulating Hormone / Q000008:administration & dosage / Q000032:analysis; D006041:Goats; D059471:In Vitro Oocyte Maturation Techniques / Q000662:veterinary; D008540:Meiosis / Q000187:drug effects / Q000502:physiology*; D009865:Oocytes / Q000166:cytology*; D006080:Ovarian Follicle / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D011994:Recombinant Proteins; D046509:Tissue Culture Techniques / Q000662:veterinary
chemical_list: D003470:Culture Media; D011994:Recombinant Proteins; D000735:Androstenedione; D004958:Estradiol; D005640:Follicle Stimulating Hormone
keywords: Androgens; Caprine; FSH; Growth rates; Secondary follicles