title: IL-10 differentially controls the infiltration of inflammatory macrophages and antigen-presenting cells during inflammation.
abstract: The inflammatory activation and recruitment of defined myeloid populations is essential for controlling the bridge between innate and adaptive immunity and shaping the immune response to microbial challenge. However, these cells exhibit significant functional heterogeneity and the inflammatory signals that differentially influence their effector characteristics are poorly characterized. In this study, we defined the phenotype of discrete subsets of effective antigen-presenting cells (APCs) in the peritoneal cavity during peritonitis. When the functional properties of these cells were compared to inflammatory monocyte-derived macrophages we noted differential responses to the immune-modulatory cytokine IL-10. In contrast to the suppressive actions of IL-10 on inflammatory macrophages, the recruitment of APCs was relatively refractory and we found no evidence for selective inhibition of APC differentiation. This differential response of myeloid cell subsets to IL-10 may thus have limited impact on development of potentially tissue-damaging adaptive immune responses, while restricting the magnitude of the inflammatory response. These findings may have clinical relevance in the context of peritoneal dialysis patients, where recurrent infections are associated with immune-mediated membrane dysfunction, treatment failure, and increased morbidity.
authors.affiliation: Division of Infection and Immunity, Cardiff University School of Medicine, Heath Park, Cardiff, UK.
authors.forename: Chia-Te
authors.initials: CT
authors.lastname: Liao
authors.affiliation: Division of Infection and Immunity, Cardiff University School of Medicine, Heath Park, Cardiff, UK.
authors.forename: Marcela
authors.initials: M
authors.lastname: Rosas
authors.affiliation: Division of Infection and Immunity, Cardiff University School of Medicine, Heath Park, Cardiff, UK.
authors.forename: Luke C
authors.initials: LC
authors.lastname: Davies
authors.affiliation: Central Biotechnology Services, Cardiff University School of Medicine, Heath Park, Cardiff, UK.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Giles
authors.affiliation: Division of Infection and Immunity, Cardiff University School of Medicine, Heath Park, Cardiff, UK.
authors.forename: Victoria J
authors.initials: VJ
authors.lastname: Tyrrell
authors.affiliation: Division of Infection and Immunity, Cardiff University School of Medicine, Heath Park, Cardiff, UK.|Systems Immunity University Research Institute, Cardiff University School of Medicine, Heath Park, Cardiff, UK.
authors.forename: Valerie B
authors.initials: VB
authors.lastname: O'Donnell
authors.affiliation: Systems Immunity University Research Institute, Cardiff University School of Medicine, Heath Park, Cardiff, UK.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Topley
authors.affiliation: Division of Infection and Immunity, Cardiff University School of Medicine, Heath Park, Cardiff, UK.|Systems Immunity University Research Institute, Cardiff University School of Medicine, Heath Park, Cardiff, UK.
authors.forename: Ian R
authors.initials: IR
authors.lastname: Humphreys
authors.affiliation: Division of Infection and Immunity, Cardiff University School of Medicine, Heath Park, Cardiff, UK.|Systems Immunity University Research Institute, Cardiff University School of Medicine, Heath Park, Cardiff, UK.
authors.forename: Donald J
authors.initials: DJ
authors.lastname: Fraser
authors.affiliation: Division of Infection and Immunity, Cardiff University School of Medicine, Heath Park, Cardiff, UK.|Systems Immunity University Research Institute, Cardiff University School of Medicine, Heath Park, Cardiff, UK.
authors.forename: Simon A
authors.initials: SA
authors.lastname: Jones
authors.affiliation: Division of Infection and Immunity, Cardiff University School of Medicine, Heath Park, Cardiff, UK. TaylorPR@cf.ac.uk.|Systems Immunity University Research Institute, Cardiff University School of Medicine, Heath Park, Cardiff, UK. TaylorPR@cf.ac.uk.
authors.forename: Philip R
authors.initials: PR
authors.lastname: Taylor
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology; D000938:Antigen-Presenting Cells / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology; D015415:Biomarkers; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism; D004195:Disease Models, Animal; D056747:Immunomodulation; D016130:Immunophenotyping; D007249:Inflammation / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism*; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D018345:Mice, Knockout; D010538:Peritonitis / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D010641:Phenotype; D054390:Receptors, CCR2 / Q000378:metabolism
chemical_list: D015415:Biomarkers; D054390:Receptors, CCR2; D016753:Interleukin-10
keywords: Antigen presenting; Antigen processing; Dendritic cells; Fate-mapping; Inflammation; Macrophages; Monocytes