title: An investigation of the antibacterial ability and cytotoxicity of a novel cu-bearing 317L stainless steel.
abstract: In order to solve the challenging problem of microbial infections caused by microorganisms on medical implants, it is imperative to develop novel antimicrobial biomaterials. This work demonstrated that 317L-Cu stainless steel (SS), created by adding copper through a solution and aging heat treatment process, exhibited good antibacterial properties against staphylococcus aureus, achieving 2 log reduction of planktonic cells after 5 days of incubation. In this study, the antibacterial test was performed using the plate count method, the fluorescence cell staining method and the quantitative polymerase chain reaction (qPCR) method. It is well known that a high concentration of copper ion can lead to cytotoxicity. This work explored the cytotoxicity of 317L-Cu SS through real-time cell analysis (RTCA). Experimental results demonstrated that the 317L-Cu SS possessed a satisfactory antibacterial ability against S. aureus, and the antibacterial rate based on the reduction of sessile cell count reached 98.3% after 24-hour treatment. The bacterial adhesion and the biofilm thickness were considerably reduced by the 317L-Cu SS. The results of RTCA suggested that 317L-Cu SS did not introduce cytotoxicity to mouse cells, indicating its suitability as a medical implant material.
authors.affiliation: Key Laboratory of Biorheological Science and Technology, College of Bioengineering, Chongqing University, Chongqing 400044, China.
authors.forename: Da
authors.initials: D
authors.lastname: Sun
authors.affiliation: Institute of Metal Research, Chinese Academy of Sciences, 72 Wenhua Road, Shenyang 110016, China.
authors.forename: Dake
authors.initials: D
authors.lastname: Xu
authors.affiliation: Institute of Metal Research, Chinese Academy of Sciences, 72 Wenhua Road, Shenyang 110016, China.
authors.forename: Chunguang
authors.initials: C
authors.lastname: Yang
authors.affiliation: Institute of Metal Research, Chinese Academy of Sciences, 72 Wenhua Road, Shenyang 110016, China.
authors.forename: M Babar
authors.initials: MB
authors.lastname: Shahzad
authors.affiliation: Institute of Metal Research, Chinese Academy of Sciences, 72 Wenhua Road, Shenyang 110016, China.
authors.forename: Ziqing
authors.initials: Z
authors.lastname: Sun
authors.affiliation: College of Chemistry, Liaoning University, Shenyang 110036, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Xia
authors.affiliation: Institute of Metal Research, Chinese Academy of Sciences, 72 Wenhua Road, Shenyang 110016, China.
authors.forename: Jinlong
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Chemical and Biomolecular Engineering, Institute for Corrosion and Multiphase Technology, Ohio University, Athens, OH 45701, USA.
authors.forename: Tingyue
authors.initials: T
authors.lastname: Gu
authors.affiliation: Institute of Metal Research, Chinese Academy of Sciences, 72 Wenhua Road, Shenyang 110016, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Yang
authors.affiliation: Key Laboratory of Biorheological Science and Technology, College of Bioengineering, Chongqing University, Chongqing 400044, China.
authors.forename: Guixue
authors.initials: G
authors.lastname: Wang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects; D001672:Biocompatible Materials / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects; D002460:Cell Line; D003300:Copper / Q000494:pharmacology; D008422:Materials Testing / Q000379:methods; D051379:Mice; D013193:Stainless Steel / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D013193:Stainless Steel; D003300:Copper