title: Comparative Study of Heparin-Poloxamer Hydrogel Modified bFGF and aFGF for in Vivo Wound Healing Efficiency.
abstract: Wound therapy remains a clinical challenge. Incorporation of growth factors (GFs) into heparin-functionalized polymer hydrogel is considered as a promising strategy to improve wound healing efficiency. However, different GFs incorporation into the same heparin-based hydrogels often lead to different wound healing effects, and the underlying GF-induced wound healing mechanisms still remain elusive. Herein, we developed a thermos-sensitive heparin-poloxamer (HP) hydrogel to load and deliver different GFs (aFGF and bFGF) for wound healing in vivo. The resulting GFs-based hydrogels with and without HP hydrogels were systematically evaluated and compared for their wound healing efficiency by extensive in vivo tests, including wound closure rate, granulation formation, re-epithelization, cell proliferation, collagen, and angiogenesis expressions. While all GFs-based dressings with and without HP hydrogels exhibited better wound healing efficacy than controls, both HP-aFGF and HP-bFGF hydrogels demonstrated their superior healing activity to improve wound closure, granulation formation, re-epithelization, and blood vessel density by up-regulation of PCNA proliferation and collagen synthesis, as compared to GF dressings alone. More importantly, HP-aFGF dressings exhibited the higher healing efficacy than HP-bFGF dressings, indicating that different a/bFGF surface properties lead to different binding and release behaviors in HP hydrogels, both of which will affect different wound healing efficiency. On the basis of experimental observations, the working mechanisms of different healing effects of HP-GFs on full skin removal wound were proposed. This work provides different views of the design and development of an effective hydrogel-based delivery system for GFs toward rapid wound healing.
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University , Wenzhou, Zhejiang 325035, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Wu
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University , Wenzhou, Zhejiang 325035, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Zhu
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University , Wenzhou, Zhejiang 325035, China.
authors.forename: Chaochao
authors.initials: C
authors.lastname: He
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University , Wenzhou, Zhejiang 325035, China.
authors.forename: Zecong
authors.initials: Z
authors.lastname: Xiao
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University , Wenzhou, Zhejiang 325035, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Ye
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University , Wenzhou, Zhejiang 325035, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University , Wenzhou, Zhejiang 325035, China.
authors.forename: Anqi
authors.initials: A
authors.lastname: Chen
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University , Wenzhou, Zhejiang 325035, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Zhang
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University , Wenzhou, Zhejiang 325035, China.
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University , Wenzhou, Zhejiang 325035, China.
authors.forename: Li
authors.initials: L
authors.lastname: Lin
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University , Wenzhou, Zhejiang 325035, China.
authors.forename: Yingzheng
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Chemical and Biomolecular Engineering, The University of Akron , Akron, Ohio 44325, United States.
authors.forename: Jie
authors.initials: J
authors.lastname: Zheng
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University , Wenzhou, Zhejiang 325035, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Xiao
mesh_terms: D016220:Fibroblast Growth Factor 1; D016222:Fibroblast Growth Factor 2; D006493:Heparin; D020100:Hydrogels / Q000737:chemistry*; D020442:Poloxamer; D014945:Wound Healing
chemical_list: D020100:Hydrogels; D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1; D020442:Poloxamer; D006493:Heparin
keywords: control release; fibroblast growth factor; heparin; hydrogel; wound dressing; wound healing