title: Prevalence and Clinical Significance of FLT3 Mutation Status in Acute Myeloid Leukemia Patients: A Multicenter Study.
abstract: FLT3-ITD mutations in acute myeloid leukemia (AML) are associated with a poor prognosis. In Latin America, little epidemiological data exist about these mutations and their influence on clinical evolution and prognosis. Standardization and well-established clinical correlation make FLT3 mutational analysis by molecular methods an invaluable tool to decide among treatment options and to determine AML prognosis.
  
  
  
  We assessed the prevalence of FLT3-ITD mutations in 138 patients with AML at four hematology referral centers from Mexico and Colombia. Molecular methods based on polymerase chain reaction (PCR) were employed for determining FLT3-ITD status.
  
  
  
  Mutations were present in 28 patients indicating a prevalence of 20.28%. Median age was 47 years (5-96). The FLT3 mutation positive group was older, had higher WBC and hemoglobin values and lower platelet counts but without statistical significance. A not previously described mutation in the FLT3 gene was found in one patient involving a nucleotide exchange of timine for cytosine at the 66608 position. A high mortality was found in the FLT3-mutated group, 67.8 vs. 42.72% in the non-mutated group and median survival was 4.9 months vs. 20.4 months, p = 0.009. A mutated FLT3 did not confer poor prognosis to those with M3 AML. The mutated FLT3 population had poor overall survival (OS) despite hematoprogenitor stem cell transplantation (HSCT).
  
  
  
  Prevalence of FLT3-ITD mutation in AML was present in a proportion comparable to other populations and, when present, was associated with a very poor prognosis.
authors.affiliation: Departamento de Hematología, División de Medicina Interna, Hospital Universiatrio "Dr. José E. González", de la facultad de Medicina de la Universidad Autónoma de Nuevo León, Monterrey, México.
authors.forename: Jorge
authors.initials: J
authors.lastname: Cuervo-Sierra
authors.affiliation: Departamento de Hematología, División de Medicina Interna, Hospital Universiatrio "Dr. José E. González", de la facultad de Medicina de la Universidad Autónoma de Nuevo León, Monterrey, México. Electronic address: carjaime@hotmail.com.
authors.forename: José Carlos
authors.initials: JC
authors.lastname: Jaime-Pérez
authors.affiliation: Departamento de Hematología, División de Medicina Interna, Hospital Universiatrio "Dr. José E. González", de la facultad de Medicina de la Universidad Autónoma de Nuevo León, Monterrey, México.
authors.forename: Ramón A
authors.initials: RA
authors.lastname: Martínez-Hernández
authors.affiliation: Departamento de Hematología, División de Medicina Interna, Hospital Universiatrio "Dr. José E. González", de la facultad de Medicina de la Universidad Autónoma de Nuevo León, Monterrey, México.
authors.forename: Ricardo D
authors.initials: RD
authors.lastname: García-Sepúlveda
authors.affiliation: Departamento de Hematología, División de Medicina Interna, Hospital Universiatrio "Dr. José E. González", de la facultad de Medicina de la Universidad Autónoma de Nuevo León, Monterrey, México.
authors.forename: Mónica
authors.initials: M
authors.lastname: Sánchez-Cárdenas
authors.affiliation: Departamento de Hematología, División de Medicina Interna, Hospital Universiatrio "Dr. José E. González", de la facultad de Medicina de la Universidad Autónoma de Nuevo León, Monterrey, México.
authors.forename: David
authors.initials: D
authors.lastname: Gómez-Almaguer
authors.affiliation: Servicio de Hematología, Unidad de Diagnóstico Molecular, Departamento de Medicina Interna, Hospital Universitario "Dr. José Eleuterio González", de la Universidad Autónoma de Nuevo León, Monterrey, México.
authors.forename: Rocío
authors.initials: R
authors.lastname: Ortíz-López
authors.affiliation: Departamento de Hematología, División de Medicina Interna, Hospital Universiatrio "Dr. José E. González", de la facultad de Medicina de la Universidad Autónoma de Nuevo León, Monterrey, México.
authors.forename: César D
authors.initials: CD
authors.lastname: Villarreal-Villarreal
authors.affiliation: Centro de Hematología y Medicina Interna, Clínica Ruiz, Puebla, México.
authors.forename: Guillermo J
authors.initials: GJ
authors.lastname: Ruiz-Arguelles
authors.affiliation: Centro de Hematología y Medicina Interna, Clínica Ruiz, Puebla, México.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Ruiz-Delgado
authors.affiliation: Centro de Hematología y Medicina Interna, Clínica Ruiz, Puebla, México.
authors.forename: Julia
authors.initials: J
authors.lastname: Lutz-Presno
authors.affiliation: Centro de Hematología y Medicina Interna, Clínica Ruiz, Puebla, México; Laboratorios Ruiz, Puebla, México and Universidad Popular Autónoma del Estado de Puebla, Puebla, México.
authors.forename: Javier
authors.initials: J
authors.lastname: Garcés-Eisele
authors.affiliation: Laboratorio de Análisis de Oncohematología (LAOH) SC, México City, México; Hospital de Oncología, Centro Medico Nacional Siglo XXI, IMSS, México City, México.
authors.forename: Gregorio
authors.initials: G
authors.lastname: Ignacio-Ibarra
authors.affiliation: Laboratorio de Análisis de Oncohematología (LAOH) SC, México City, México; Hospital General de México, México City, México.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Muciño-Hernández
authors.affiliation: Laboratorio de Análisis de Oncohematología (LAOH) SC, México City, México; Hospital General de México, México City, México.
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Arana-Trejo
authors.affiliation: Unidad de Genética Médica, Facultad de Medicina de la Universidad de Antioquia, Medellín, Colombia.
authors.forename: Angélica M
authors.initials: AM
authors.lastname: Jiménez-Mejia
authors.affiliation: Unidad de Genética Médica, Facultad de Medicina de la Universidad de Antioquia, Medellín, Colombia.
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Vásquez-Palacio
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D004252:DNA Mutational Analysis; D005260:Female; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000175:diagnosis / Q000201:enzymology*; D008297:Male; D008800:Mexico; D008875:Middle Aged; D009154:Mutation; D011379:Prognosis; D055815:Young Adult; D051941:fms-Like Tyrosine Kinase 3 / Q000235:genetics / Q000378:metabolism*
chemical_list: C497970:FLT3 protein, human; D051941:fms-Like Tyrosine Kinase 3
keywords: AML; Acute myeloid leukemia; FLT3; Latin America