title: Column Chromatography To Obtain Organic Cation Sorption Isotherms.
abstract: Column chromatography was evaluated as a method to obtain organic cation sorption isotherms for environmental solids while using the peak skewness to identify the linear range of the sorption isotherm. Custom packed HPLC columns and standard batch sorption techniques were used to intercompare sorption isotherms and solid-water sorption coefficients (Kd) for four organic cations (benzylamine, 2,4-dichlorobenzylamine, phenyltrimethylammonium, oxytetracycline) with two aluminosilicate clay minerals and one soil. A comparison of Freundlich isotherm parameters revealed isotherm linearity or nonlinearity was not significantly different between column chromatography and traditional batch experiments. Importantly, skewness (a metric of eluting peak symmetry) analysis of eluting peaks can establish isotherm linearity, thereby enabling a less labor intensive means to generate the extensive data sets of linear Kd values required for the development of predictive sorption models. Our findings clearly show that column chromatography can reproduce sorption measures from conventional batch experiments with the benefit of lower labor-intensity, faster analysis times, and allow for consistent sorption measures across laboratories with distinct chromatography instrumentation.
authors.affiliation: Department of Civil and Environmental Engineering, University of Connecticut , Storrs, Connecticut 06269, United States.
authors.forename: William C
authors.initials: WC
authors.lastname: Jolin
authors.affiliation: Department of Chemistry, Bowdoin College , Brunswick, Maine 04011, United States.
authors.forename: James
authors.initials: J
authors.lastname: Sullivan
authors.affiliation: Department of Chemistry, Bowdoin College , Brunswick, Maine 04011, United States.
authors.forename: Dharni
authors.initials: D
authors.lastname: Vasudevan
authors.affiliation: Department of Civil, Environmental and Geodetic Engineering, The Ohio State University , Columbus, Ohio 43210, United States.
authors.forename: Allison A
authors.initials: AA
authors.lastname: MacKay
mesh_terms: D000327:Adsorption; D002412:Cations / Q000737:chemistry*; D002845:Chromatography; D012987:Soil / Q000737:chemistry*; D012989:Soil Pollutants
chemical_list: D002412:Cations; D012987:Soil; D012989:Soil Pollutants