title: Synthesis, spectral characterization, DNA interaction, anticancer and molecular docking studies on some transition metal complexes with bidentate ligand.
abstract: The ligand, N(2),N(3)-bis(3-nitrophenyl)quinoxaline-2.3-diamine was prepared by the condensation of quinoxaline-2.3(1,4H)-dione with 3-nitroaniline. It was treated with Co(II), Ni(II), Cu(II) and Zn(II) acetates to form the metal complexes. These were characterized by elemental analysis, molar conductance, magnetic moment, UV-Vis., IR, (1)H NMR, ESR and mass spectral data. Octahedral geometry has been assigned to Co(II), Ni(II) and Zn(II) complexes, whereas Cu(II) complex has distorted octahedral geometry. From the powder XRD data, crystallite size and unit cell parameters were calculated. The surface morphology of the synthesized compounds were determined using SEM analysis. The antimicrobial activity of the compounds against some bacterial species viz. Escherichiacoli, Klebsiella pneumoniae, Pseudomonas aeuruginosa and Staphylococcus aureus; also the fungal species, Aspergillus niger, and Candida albicans were done by disc diffusion method. DNA binding, cleavage and super oxide anion scavenging activities were also evaluated. The DNA binding activity of the compounds were identified using electronic absorption titrations and DNA cleavage was determined using gel electrophoresis. The anticancer activities of the compounds against HeLa cell line were determined using MTT assay. The highly potent compound among the five against HeLa cell line is subjected to molecular docking study against human papilloma virus receptor molecule and ATP binding site of telomerase.
authors.affiliation: Department of Chemistry, University College of Engineering, Nagercoil (Anna University Constituent College), Nagercoil, 629004, Tamil Nadu, India. Electronic address: c.justindhanaraj@gmail.com.
authors.forename: C Justin
authors.initials: CJ
authors.lastname: Dhanaraj
authors.affiliation: Department of Research, College of Art and Applied Sciences, Dhofar University, P.O. Box 2509, PC 211 Salalah,Oman.
authors.forename: Israr Ul
authors.initials: IU
authors.lastname: Hassan
authors.affiliation: Department of Chemistry, University College of Engineering, Nagercoil (Anna University Constituent College), Nagercoil, 629004, Tamil Nadu, India.
authors.forename: Jijo
authors.initials: J
authors.lastname: Johnson
authors.affiliation: Department of Chemistry, Noorul Islam University, Kumaracoil, 629 180, Tamil Nadu, India.
authors.forename: J
authors.initials: J
authors.lastname: Joseph
authors.affiliation: Department of Chemistry, Mar Ivanios College (Autonomous), Thiruvananthapuram, 695015, Kerala, India.
authors.forename: R Selwin
authors.initials: RS
authors.lastname: Joseyphus
mesh_terms: D000890:Anti-Infective Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D018360:Crystallography, X-Ray; D004247:DNA / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D008024:Ligands; D008855:Microscopy, Electron, Scanning; D062105:Molecular Docking Simulation; D013057:Spectrum Analysis / Q000379:methods; D013482:Superoxide Dismutase / Q000378:metabolism; D028561:Transition Elements / Q000737:chemistry*
chemical_list: D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D008024:Ligands; D028561:Transition Elements; D004247:DNA; D013482:Superoxide Dismutase
keywords: Anticancer; Antimicrobial; DNA; Molecular docking; Quinoxaline