title: Identification and expression profiling of DNA methyltransferases during development and stress conditions in Solanaceae.
abstract: DNA methyltransferase (DMTase) enzymes contribute to plant development and stress responses by de novo establishment and subsequent maintenance of DNA methylation during replication. However, the molecular mechanism underlying this activity remains obscure, especially in crop species. Using DMTase homolog complement in six Solanaceae species, we demonstrated here that their number remained conserved in Solanum lineage, whereas it was expanded in both pepper and Nicotiana benthamiana. Non-synonymous vs synonymous (Ka/Ks) substitution ratio revealed that most of the Solanaceous DMTase homologs undergo purifying selection. The genomic sequences of tomato DMT homologs in its wild relative, Solanum pennellii, remained highly conserved in their exons and methyltransferase domains. Structure analysis further revealed highly similar folding of DMTase homologs and conservation in the residues participating in protein-protein interaction in Solanum lineage, whereas a considerable diversification was observed of pepper homologs. Transcript profiling of DMTases highlighted both similar and distinct expression patterns of tomato homologs in other species during fruit development and stress responses. Overall, our analysis provides a strong basis for in-depth exploration of both conserved as well as distinct functions of tomato DMTase homologs in other economically important Solanaceae species.
authors.affiliation: Repository of Tomato Genomic Resources, Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, Telangana, 500046, India. rahulpmb@gmail.com.
authors.forename: Rahul
authors.initials: R
authors.lastname: Kumar
authors.affiliation: Repository of Tomato Genomic Resources, Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, Telangana, 500046, India.
authors.forename: Pankaj Kumar
authors.initials: PK
authors.lastname: Chauhan
authors.affiliation: Botany Department, Zakir Husain Delhi College, University of Delhi, New Delhi, 110002, India.
authors.forename: Ashima
authors.initials: A
authors.lastname: Khurana
mesh_terms: D019175:DNA Methylation / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D008780:Methyltransferases / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D020411:Oligonucleotide Array Sequence Analysis; D010802:Phylogeny; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D019657:Solanaceae / Q000235:genetics* / Q000254:growth & development
chemical_list: D018744:DNA, Plant; D010940:Plant Proteins; D008780:Methyltransferases
keywords: DNA methyltransferase; Gene family; Solanum lineage; Tomato; Transcript profiling