title: Bacoside-A, an anti-amyloid natural substance, inhibits membrane disruption by the amyloidogenic determinant of prion protein through accelerating fibril formation.
abstract: Bacosides, class of compounds extracted from the Bacopa monniera plant, exhibit interesting therapeutic properties, particularly enhancing cognitive functions and putative anti-amyloid activity. We show that bacoside-A exerted significant effects upon fibrillation and membrane interactions of the amyloidogenic fragment of the prion protein [PrP(106-126)]. Specifically, when co-incubated with PrP(106-126), bacoside-A accelerated fibril formation in the presence of lipid bilayers and in parallel inhibited bilayer interactions of the peptide aggregates formed in solution. These interesting phenomena were studied by spectroscopic and microscopic techniques, which suggest that bacoside A-promoted fibrillation reduced the concentration of membrane-active pre-fibrillar species of the prion fragment. This study suggests that induction of fibril formation and corresponding inhibition of membrane interactions are likely the underlying factors for ameliorating amyloid protein toxicity by bacoside-A.
authors.affiliation: Department of Chemistry, Ben Gurion University of the Negev, Beer Sheva 84105, Israel.
authors.forename: Ravit
authors.initials: R
authors.lastname: Malishev
authors.affiliation: Department of Chemistry, Ben Gurion University of the Negev, Beer Sheva 84105, Israel.
authors.forename: Sukhendu
authors.initials: S
authors.lastname: Nandi
authors.affiliation: Ilse Katz Institute for Nanotechnology, Ben Gurion University of the Negev, Beer Sheva 84105, Israel.
authors.forename: Sofiya
authors.initials: S
authors.lastname: Kolusheva
authors.affiliation: Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel.
authors.forename: Shira
authors.initials: S
authors.lastname: Shaham-Niv
authors.affiliation: Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel; Department of Materials Science and Engineering, Iby and Aladar Fleischman Faculty of Engineering, Tel Aviv University, Tel Aviv 69978, Israel.
authors.forename: Ehud
authors.initials: E
authors.lastname: Gazit
authors.affiliation: Department of Chemistry, Ben Gurion University of the Negev, Beer Sheva 84105, Israel; Ilse Katz Institute for Nanotechnology, Ben Gurion University of the Negev, Beer Sheva 84105, Israel. Electronic address: razj@bgu.ac.il.
authors.forename: Raz
authors.initials: R
authors.lastname: Jelinek
mesh_terms: D000682:Amyloid / Q000037:antagonists & inhibitors / Q000737:chemistry*; D008567:Membranes, Artificial; D000072002:Prion Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry*; D014315:Triterpenes / Q000737:chemistry*
chemical_list: D000682:Amyloid; D008567:Membranes, Artificial; D000072002:Prion Proteins; D012503:Saponins; D014315:Triterpenes; C500175:bacoside A
keywords: Amyloid fibrils; Amyloid peptides; Bacoside; Membrane interactions; PrP(106–126); Prion protein