title: Hesperidin Alleviates Lipopolysaccharide-Induced Neuroinflammation in Mice by Promoting the miRNA-132 Pathway.
abstract: Previous studies have demonstrated that hesperidin, a flavanone glycoside from citrus fruits, produces antidepressant-like effects in both mice and rats. However, whether these effects are mediated by pro-inflammatory cytokines remains unknown. In the present study, we attempted to investigate the effects of hesperidin on the depressive-like behavior; the serum corticosterone concentrations; and the interleukin (IL)-1β, IL-6, and tumor necrosis factor alpha (TNF-α) levels in lipopolysaccharide (LPS)-induced depression-like mice. In particular, we evaluated the miRNA-132 expression after LPS and hesperidin treatment. We found that LPS injection not only decreased the sucrose preference and increased the serum corticosterone levels but also elevated IL-1β, IL-6, and TNF-α in the prefrontal cortex. More importantly, LPS down-regulated the expression of miRNA-132. Pre-treatment with hesperidin (25, 50, 100 mg/kg) for 7 days prevented these abnormalities induced by LPS injection. In contrast, this effect of hesperidin was abolished by a miRNA-132 antagomir. Taken together, these results suggest that the antidepressant-like mechanisms of hesperidin are at least partially related to decreased pro-inflammatory cytokine levels via the miRNA-132 pathway in the brain.
authors.affiliation: Central ICU, Henan Province People's Hospital, Zhengzhou, 450003, Henan Province, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.affiliation: Central ICU, Henan Province People's Hospital, Zhengzhou, 450003, Henan Province, PR China.
authors.forename: Huanzhang
authors.initials: H
authors.lastname: Shao
authors.affiliation: Central ICU, Henan Province People's Hospital, Zhengzhou, 450003, Henan Province, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Central ICU, Henan Province People's Hospital, Zhengzhou, 450003, Henan Province, PR China. hnsyicu2@sina.com.
authors.forename: Bingyu
authors.initials: B
authors.lastname: Qin
mesh_terms: D000818:Animals; D000928:Antidepressive Agents; D001923:Brain Chemistry / Q000187:drug effects*; D016207:Cytokines / Q000187:drug effects; D003863:Depression / Q000188:drug therapy; D004305:Dose-Response Relationship, Drug; D006569:Hesperidin / Q000494:pharmacology*; D007249:Inflammation / Q000188:drug therapy*; D008070:Lipopolysaccharides / Q000494:pharmacology; D053858:Metabolic Networks and Pathways; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*
chemical_list: D000928:Antidepressive Agents; D016207:Cytokines; D008070:Lipopolysaccharides; C546976:MIRN132 microRNA, mouse; D035683:MicroRNAs; D006569:Hesperidin
keywords: Antidepressant; Hesperidin; Lipopolysaccharide (LPS); Pro-inflammatory cytokines; miRNA-132