title: Secondary metabolites from marine-derived Streptomyces antibioticus strain H74-21.
abstract: A new secondary metabolite, (2S,3R)-l-threonine, N-[3-(formylamino)-2-hydroxybenzoyl]-ethyl ester (streptomyceamide C, 1), together with four known compounds 1, 4-dimethyl-3-isopropyl-2,5-piperidinedione (2), cyclo-((S)-Pro-8- hydroxy-(R)-Ile (3), cyclo-((S)-Pro-(R)-Leu (4), and seco-((S)-Pro-(R)-Val) (5), were isolated from the EtOH extract of the fermented mycelium of the marine-derived streptomycete strain H74-21, which was isolated from sea sediment in a mangrove site. The structure of the new compound was established on the basis of its spectroscopic data, including 1D and 2D NMR, HR-TOF-MS. Their antifungal activities against Candida albicans and cytotoxicities against human breast adenocarcinoma cell line MCF-7, human glioblastoma cell line SF-268 and human lung cancer cell line NCI-H460 were tested. Compounds 1 only displayed cytotoxicity against human breast adenocarcinoma cell line MCF-7 with the IC50 value of 27.0 μg/mL. However, compounds 1-5 do not show antifungal activities at the test concentration of 1 mg/mL, and 2-5 have no cytotoxicities at the test concentration of 50 μg/mL.
authors.affiliation: a Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine and New Drugs Research, Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy , Jinan University , Guangzhou , China.
authors.forename: Shuna
authors.initials: S
authors.lastname: Fu
authors.affiliation: a Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine and New Drugs Research, Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy , Jinan University , Guangzhou , China.
authors.forename: Fan
authors.initials: F
authors.lastname: Wang
authors.affiliation: a Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine and New Drugs Research, Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy , Jinan University , Guangzhou , China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Li
authors.affiliation: a Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine and New Drugs Research, Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy , Jinan University , Guangzhou , China.
authors.forename: Yixuan
authors.initials: Y
authors.lastname: Bao
authors.affiliation: a Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine and New Drugs Research, Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy , Jinan University , Guangzhou , China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Yang
authors.affiliation: b Key Laboratory of New Technique for Plant Protection in Guangdong, Institute of Plant Protection , Guangdong Academy of Agricultural Sciences , Guangzhou , China.
authors.forename: Huifang
authors.initials: H
authors.lastname: Shen
authors.affiliation: b Key Laboratory of New Technique for Plant Protection in Guangdong, Institute of Plant Protection , Guangdong Academy of Agricultural Sciences , Guangzhou , China.
authors.forename: Birun
authors.initials: B
authors.lastname: Lin
authors.affiliation: a Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine and New Drugs Research, Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy , Jinan University , Guangzhou , China.
authors.forename: Guangxiong
authors.initials: G
authors.lastname: Zhou
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects; D005285:Fermentation; D019015:Geologic Sediments / Q000382:microbiology*; D006801:Humans; D061986:MCF-7 Cells; D064210:Secondary Metabolism; D013302:Streptomyces / Q000378:metabolism*
chemical_list: D000903:Antibiotics, Antineoplastic; D000935:Antifungal Agents
keywords: Streptomycete; dipeptide; piperidinedione; secondary metabolites; streptomyceamide C