title: A Versatile Gene Delivery System for Efficient and Tumor Specific Gene Manipulationin vivo.
abstract: The Replication-Competent Avian Sarcoma-leukosis virus long-terminal repeat with splice acceptor (RCAS)-Tumor Virus A (TVA) gene delivery system has been created based on the fact that avian sarcoma leukosis virus subgroup A only infects cells expressing its receptor, TVA. This system has been successfully applied to create various mouse models for human cancers. Here we briefly discuss the advantages and the potential caveats of using this RCAS-TVA gene delivery system in cancer research. We also introduce and discuss how our newly designed RCAS-based gene delivery system (RCI-Oncogene, for RCAS-Cre-IRES-Oncogene) allows concise and efficient manipulation of gene expression in tumors in vivo, and how this system can be used to rapidly study the biological function of gene(s) and/or the collaborative actions of multiple genes in regulating tumor initiation, progression and/or metastasis.
authors.affiliation: Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, 77030, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, 77030, USA.
authors.forename: Bingning
authors.initials: B
authors.lastname: Dong
authors.affiliation: Department of Pathology and Immunology, Baylor College of Medicine, Houston, TX, 77030, USA.
authors.forename: Michael M
authors.initials: MM
authors.lastname: Ittmann
authors.affiliation: Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX, 77030, USA.
authors.forename: Feng
authors.initials: F
authors.lastname: Yang
keywords: Cre; RCAS; RCI; RCI-Oncogene; RCI-PyMT; TVA; knockout