title: Inhibitory effect on HT-29 colon cancer cells of a water-soluble polysaccharide obtained from highland barley.
abstract: A water-soluble polysaccharide (BP-1) was obtained from highland barley (Hordeum vulgare L.) by hot water extraction and purification of sepharose column chromatography. BP-1 had an average molecular weight of about 6.7×104Da and was composed of glucose (Glc), xylose (Xyl), arabinose (Ara) and rhamnose (Rha) with a relative molar ratio of 8.82:1.92:1.50:1.00. It was found that BP-1 inhibited proliferation of human colon cancer cells (HT-29) in a time- and dose-dependent manner with half maximal inhibitory concentration at 48h of 48.18μg/mL. Western blotting results showed that BP-1 enhanced the phosphorylation of c-Jun N-terminal kinase (JNK), processes associated with the reactive oxygen species (ROS) formation and inhibited nuclear factor-κB (NF-κB) translocation from cytoplasm into nucleus. Meanwhile, the BP-1-induced apoptosis was related to the regulation of apoptosis-associated proteins, such as B-cell lymphoma-2 (Bcl-2), release of cytochrome C from mitochondria to cytoplasm and activation of caspase-8 and caspase-9. These results suggest that BP-1-induced HT-29 apoptosis through ROS-JNK and NF-κB-mediated caspase pathways.
authors.affiliation: Key Laboratory of Food Safety and Sanitation, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, People's Republic of China.
authors.forename: Dai
authors.initials: D
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Food Safety and Sanitation, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, People's Republic of China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Food Safety and Sanitation, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, People's Republic of China.
authors.forename: Meng
authors.initials: M
authors.lastname: Meng
authors.affiliation: Key Laboratory of Food Safety and Sanitation, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, People's Republic of China.
authors.forename: Lirong
authors.initials: L
authors.lastname: Han
authors.affiliation: Key Laboratory of Food Safety and Sanitation, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, People's Republic of China.
authors.forename: Caijiao
authors.initials: C
authors.lastname: Li
authors.affiliation: Key Laboratory of Food Safety and Sanitation, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, People's Republic of China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Hou
authors.affiliation: Academy of State Administration of Grain, No.11 Baiwanzhuang Avenue, Xicheng District, Beijing, 100037, People's Republic of China.
authors.forename: Wentao
authors.initials: W
authors.lastname: Qi
authors.affiliation: Key Laboratory of Food Safety and Sanitation, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, People's Republic of China. Electronic address: wangchunling@tust.edu.cn.
authors.forename: Chunling
authors.initials: C
authors.lastname: Wang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D001467:Hordeum / Q000737:chemistry*; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D012995:Solubility
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D009363:Neoplasm Proteins; D011134:Polysaccharides; D017382:Reactive Oxygen Species
keywords: Anticancer; Barley; Human colon cancer; Polysaccharide