title: Complete genome sequence of tobacco mosqueado virus.
abstract: We describe the genomic characteristics of a new potyvirus isolated from tobacco plants showing mottling ("mosqueado" in Portuguese) in southern Brazil. The complete genomic sequence consists of 9896 nucleotides, without the poly(A) tail, and shares the highest pairwise nucleotide sequence identities of 68.5 % with pepper yellow mosaic virus and 68.2 % with Brugmansia mosaic virus isolate D437. These identity values are below the level of 76.0 % used as a criterion for species demarcation in the genus Potyvirus based on the complete genome sequence. The viral genomic organization and sequence comparison thus suggest that this virus, tentatively named "tobacco mosqueado virus" (TMosqV), represents a new potyvirus species.
authors.affiliation: Department of Cellular Biology, University of Brasília, Campus Darcy Ribeiro, Brasilia, DF, 70910-900, Brazil.
authors.forename: Rosana
authors.initials: R
authors.lastname: Blawid
authors.affiliation: Department of Cellular Biology, University of Brasília, Campus Darcy Ribeiro, Brasilia, DF, 70910-900, Brazil.
authors.forename: Kelly Barreto
authors.initials: KB
authors.lastname: Rodrigues
authors.affiliation: Department of Plant Pathology, University of Brasília, Campus Darcy Ribeiro, Brasilia, DF, 70910-900, Brazil.
authors.forename: Camila
authors.initials: C
authors.lastname: de Moraes Rêgo
authors.affiliation: Department of Plant Pathology, University of Brasília, Campus Darcy Ribeiro, Brasilia, DF, 70910-900, Brazil.|Embrapa Vegetables, Caixa Postal 218, Brasilia, DF, 70359-970, Brazil.
authors.forename: Alice K
authors.initials: AK
authors.lastname: Inoue-Nagata
authors.affiliation: Department of Cellular Biology, University of Brasília, Campus Darcy Ribeiro, Brasilia, DF, 70910-900, Brazil. tatsuya@unb.br.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Nagata
mesh_terms: D001483:Base Sequence; D021141:Nucleic Acid Amplification Techniques; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D010942:Plant Viruses / Q000235:genetics*; D017800:Potyvirus / Q000235:genetics* / Q000302:isolation & purification*; D012330:RNA, Double-Stranded / Q000235:genetics; D014026:Nicotiana / Q000821:virology*
chemical_list: D012330:RNA, Double-Stranded