title: Kato-Katz and Lumbreras rapid sedimentation test to evaluate helminth prevalence in the setting of a school-based deworming program.
abstract: The sensitivity of the Kato-Katz test is suboptimal for the evaluation of intestinal helminth prevalence. Moreover, during mass deworming, as helminth egg burden decreases, the sensitivity is likely to decrease. The Lumbreras rapid sedimentation (Lumbreras) is a low-cost non-quantitative test, but may provide useful information in low burden areas. We compared the prevalence of intestinal helminth infections assessed by the Kato-Katz and the Lumbreras rapid sedimentation test on 3 stool specimens from each of 1083 children. The sensitivities were compared using the McNemar paired test. Using the combined outcome of the 3 different stool tests as the standard, Kato-Katz had lower sensitivity than Lumbreras rapid sedimentation tests for Ascaris lumbricoides (85.1% vs. 95.1%, p = 0.03), Hymenolepis nana (77.7% vs. 97.9%, p < 0.01), Trichuris trichura (41.7% vs. 100%, p = 0.01), hookworm (0% vs. 100%, p = 0.01), and Strongyloides stercoralis (0% vs. 88%, p < 0.01). Kato-Katz demonstrated significantly lower sensitivity, missing most T. trichiura, hookworm, and S. stercoralis infections. The combination of Kato-Katz and Lumbreras rapid sedimentation tests enables the detection of more intestinal helminths infections in post-deworming low prevalence areas.
authors.affiliation: a Universidad Peruana Cayetano Heredia and University of Texas Medical Branch Collaborative Research Center , Cusco , Peru.
authors.forename: Martha
authors.initials: M
authors.lastname: Lopez
authors.affiliation: a Universidad Peruana Cayetano Heredia and University of Texas Medical Branch Collaborative Research Center , Cusco , Peru.
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Morales
authors.affiliation: b School of Medicine , University of Texas Medical Branch , Galveston , TX , USA.
authors.forename: Monisha
authors.initials: M
authors.lastname: Konana
authors.affiliation: b School of Medicine , University of Texas Medical Branch , Galveston , TX , USA.
authors.forename: Paige
authors.initials: P
authors.lastname: Hoyer
authors.affiliation: a Universidad Peruana Cayetano Heredia and University of Texas Medical Branch Collaborative Research Center , Cusco , Peru.
authors.forename: Roberto
authors.initials: R
authors.lastname: Pineda-Reyes
authors.affiliation: a Universidad Peruana Cayetano Heredia and University of Texas Medical Branch Collaborative Research Center , Cusco , Peru.|c Infectious Disease Division, Department of Internal Medicine , University of Texas Medical Branch , Galveston , TX , USA.
authors.forename: Arthur Clinton
authors.initials: AC
authors.lastname: White
authors.affiliation: d Cysticercosis Working Group in Peru , Lima , Peru.|e Faculty of Science and Philosophy, Infectious Diseases Laboratory Research-LID, Alberto Cazorla Talleri , Universidad Peruana Cayetano Heredia , Lima , Peru.|f Cysticercosis Unit , Instituto de Ciencias Neurologicas , Lima , Peru.
authors.forename: Hector Hugo
authors.initials: HH
authors.lastname: Garcia
authors.affiliation: g School of Public Health and Administration , Universidad Peruana Cayetano Heredia , Lima , Peru.|h US Naval Medical Research Unit No. 6 (NAMRU-6) , Lima , Peru.
authors.forename: Andres Guillermo
authors.initials: AG
authors.lastname: Lescano
authors.affiliation: i Instituto de Medicina Tropical Alexander von Humboldt , Universidad Peruana Cayetano Heredia , Lima , Peru.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Gotuzzo
authors.affiliation: a Universidad Peruana Cayetano Heredia and University of Texas Medical Branch Collaborative Research Center , Cusco , Peru.|c Infectious Disease Division, Department of Internal Medicine , University of Texas Medical Branch , Galveston , TX , USA.|i Instituto de Medicina Tropical Alexander von Humboldt , Universidad Peruana Cayetano Heredia , Lima , Peru.
authors.forename: Miguel Mauricio
authors.initials: MM
authors.lastname: Cabada
mesh_terms: D000818:Animals; D017164:Ascaris lumbricoides / Q000302:isolation & purification*; D002648:Child; D002675:Child, Preschool; D019411:Clinical Laboratory Techniques; D005243:Feces / Q000469:parasitology*; D005260:Female; D006373:Helminthiasis / Q000175:diagnosis / Q000453:epidemiology* / Q000469:parasitology*; D006801:Humans; D007411:Intestinal Diseases, Parasitic / Q000175:diagnosis / Q000453:epidemiology* / Q000469:parasitology*; D008297:Male; D010270:Parasite Egg Count; D015995:Prevalence; D012572:School Health Services; D012574:Schools; D012680:Sensitivity and Specificity
keywords: Kato-Katz; Lumbreras rapid sedimentation; Screening tests; Sensitivity; Soil-transmitted helminths