title: TNF-α-308/-238 polymorphisms are associated with gastric cancer: A case-control family study in China.
abstract: BACKGROUND
  Associations of TNF-α-308 (rs1800629) and -238 (rs361525) with gastric cancer had the inconsistent indication among different populations.
  
  
  METHODS
  In this case-control family study, 47 families were determined with the probands diagnosed with gastric cancer (case family, n=296), accordingly 47 families without gastric cancer were matched with the case families by multivariate distribution of age, sex, social class, and pedigree size (control family, n=319). Polymerase chain reaction-restriction fragment length polymorphism (RFLP-PCR) was used to identify the TNF genotype. Chi-square test was used to compare the groups regarding genotype and the allele frequencies, HWE test for Hardy-Weinberg equilibrium.
  
  
  RESULTS
  The frequencies of TNF-α-308 GA and AA genotypes were significantly higher in case family than that in control family. The risk of gastric cancer was increased in GA and AA carriers in the first degree (OR=2.06, 95% CI=1.20-3.51 and OR=4.89, 95% CI=2.74-8.74), however the similar result was not found in the second degree. Helicobacter pylori infection status were significantly associated with risk of gastric cancer in first-degree relatives (OR=1.96, 95% CI=1.26-3.05) while no statistical significance was noted in the second-degree relatives. Haplotypes of TNF-α-308/-238 alleles, GA/GG, AA/GG and AA/GA indicated the susceptibilities to gastric cancer with OR and 95% confident intervals resulting 2.07 (1.34-3.21), 4.49 (2.74-7.33) and 4.98 (1.76-14.01) respectively.
  
  
  CONCLUSIONS
  TNF-α-G308A (rs1800629) polymorphisms are associated with gastric cancer in Chinese population. Haplotypes of TNF-α-308/-238 GA/GG, AA/GG and AA/GA increase the susceptibilities to gastric cancer. The first-degree relatives are more likely to develop into gastric cancer with TNF-α-G308 polymorphisms and H. pylori positive than the second-degree are.
authors.affiliation: Zhengzhou University, College of Public Health, Zhengzhou 450001, Henan, PR China; Key Laboratory of Tumor Epidemiology of Henan Province, Zhengzhou 450052, PR China.
authors.forename: Yajuan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Zhengzhou University, College of Public Health, Zhengzhou 450001, Henan, PR China; Key Laboratory of Tumor Epidemiology of Henan Province, Zhengzhou 450052, PR China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Cao
authors.affiliation: Zhengzhou University, College of Public Health, Zhengzhou 450001, Henan, PR China; Key Laboratory of Tumor Epidemiology of Henan Province, Zhengzhou 450052, PR China.
authors.forename: Jicheng
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Zhengzhou University, College of Public Health, Zhengzhou 450001, Henan, PR China; Key Laboratory of Tumor Epidemiology of Henan Province, Zhengzhou 450052, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Zhengzhou University, College of Public Health, Zhengzhou 450001, Henan, PR China; Key Laboratory of Tumor Epidemiology of Henan Province, Zhengzhou 450052, PR China. Electronic address: kjwang@163.com.
authors.forename: Kaijuan
authors.initials: K
authors.lastname: Wang
mesh_terms: D000230:Adenocarcinoma / Q000208:ethnology / Q000235:genetics*; D000328:Adult; D044466:Asian People / Q000706:statistics & numerical data; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D005190:Family; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease; D005838:Genotype; D016481:Helicobacter Infections / Q000150:complications*; D016480:Helicobacter pylori / Q000472:pathogenicity*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010375:Pedigree; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000208:ethnology / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: D014409:Tumor Necrosis Factor-alpha