title: Polyphenolic responses of grapevine berries to light, temperature, oxidative stress, abscisic acid and jasmonic acid show specific developmental-dependent degrees of metabolic resilience to perturbation.
abstract: Grape-berries are exposed to a plethora of abiotic and biotic stimuli during their development. The developmental and temporal regulation of grape berry polyphenol metabolism in response to various cues was investigated using LC-QTOF-MS based metabolite profiling. High light (2500μmolm(-2)s(-1)), high temperature (40°C), jasmonic acid (200μM), menadione (120μM) and abscisic acid (3.026mM) treatments were applied to detached berries. Greater magnitudes of metabolite fluctuations characterize the pre-veraison berries than the veraison stage in response to the treatments. Furthermore, a tighter co-response of metabolic processes was shown at veraison, likely supporting the resilience to change in response to stress. High temperature and ABA treatments led to greater magnitudes of change during the course of the experiment. The present study demonstrates the occurrence of differential patterns of metabolic responses specific to individual cues and berry developmental stage, which in the field are commonly associated and thus hardly discernable.
authors.affiliation: The French Associates Institute for Agriculture and Biotechnology of Drylands, The Jacob Blaustein Institute for Desert Research, Ben-Gurion University of the Negev, Sede Boqer, Israel. Electronic address: degua3@gmail.com.
authors.forename: Asfaw
authors.initials: A
authors.lastname: Degu
authors.affiliation: The French Associates Institute for Agriculture and Biotechnology of Drylands, The Jacob Blaustein Institute for Desert Research, Ben-Gurion University of the Negev, Sede Boqer, Israel. Electronic address: birukayenew@gmail.com.
authors.forename: Biruk
authors.initials: B
authors.lastname: Ayenew
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Nevada, Reno, NV 9557, USA. Electronic address: cramer@unr.edu.
authors.forename: Grant R
authors.initials: GR
authors.lastname: Cramer
authors.affiliation: The French Associates Institute for Agriculture and Biotechnology of Drylands, The Jacob Blaustein Institute for Desert Research, Ben-Gurion University of the Negev, Sede Boqer, Israel. Electronic address: fait@bgu.ac.il.
authors.forename: Aaron
authors.initials: A
authors.lastname: Fait
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology*; D003517:Cyclopentanes / Q000494:pharmacology*; D005638:Fruit / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects; D008027:Light; D055442:Metabolome / Q000187:drug effects / Q000528:radiation effects; D018384:Oxidative Stress; D054883:Oxylipins / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000494:pharmacology; D059808:Polyphenols / Q000378:metabolism*; D013696:Temperature; D027843:Vitis / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects
chemical_list: D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D059808:Polyphenols; C011006:jasmonic acid; D000040:Abscisic Acid
keywords: Environmental cues; Grape detached berry; LC-QTOF-MS; Metabolite profiling; Phenylpropanoid pathway; Pre-veraison; Veraison