title: Biomimetic nanochannels based biosensor for ultrasensitive and label-free detection of nucleic acids.
abstract: A very simple sensing device based on biomimetic nanochannels has been developed for label-free, ultrasensitive and highly sequence-specific detection of DNA. Probe DNA was modified on the inner wall of the nanochannel surface by layer-by-layer (LBL) assembly. After probe DNA immobilization, DNA detection was realized by monitoring the rectified ion current when hybridization occurred. Due to three dimensional (3D) nanoscale environment of the nanochannel, this special geometry dramatically increased the surface area of the nanochannel for immobilization of probe molecules on the inner-surface and enlarged contact area between probes and target-molecules. Thus, the unique sensor reached a reliable detection limit of 10 fM for target DNA. In addition, this DNA sensor could discriminate complementary DNA (c-DNA) from non-complementary DNA (nc-DNA), two-base mismatched DNA (2bm-DNA) and one-base mismatched DNA (1bm-DNA) with high specificity. Moreover, the nanochannel-based biosensor was also able to detect target DNA even in an interfering environment and serum samples. This approach will provide a novel biosensing platform for detection and discrimination of disease-related molecular targets and unknown sequence DNA.
authors.affiliation: School of Laboratory Medicine, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, China.
authors.forename: Zhongyue
authors.initials: Z
authors.lastname: Sun
authors.affiliation: School of Laboratory Medicine, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, China.
authors.forename: Tangbin
authors.initials: T
authors.lastname: Liao
authors.affiliation: School of Laboratory Medicine, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, China.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Nuring, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Shu
authors.affiliation: Teaching and Research Office of Forensic Medicine, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, China. Electronic address: redguo@hotmail.com.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: School of Laboratory Medicine, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, China. Electronic address: zhanggj@hbtcm.edu.cn.
authors.forename: Guo-Jun
authors.initials: GJ
authors.lastname: Zhang
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry*; D003217:Conductometry / Q000295:instrumentation*; D004247:DNA / Q000032:analysis* / Q000235:genetics*; D015342:DNA Probes / Q000737:chemistry / Q000235:genetics; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007473:Ion Channels / Q000737:chemistry*; D015336:Molecular Probe Techniques / Q000295:instrumentation; D058608:Nanopores / Q000648:ultrastructure; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000295:instrumentation; D013194:Staining and Labeling
chemical_list: D015342:DNA Probes; D007473:Ion Channels; D004247:DNA
keywords: Biosensor; DNA; Hybridization; Label-free detection; Nanochannels