title: The Combined Effects of Ethylene and MeJA on Metabolic Profiling of Phenolic Compounds in Catharanthus roseus Revealed by Metabolomics Analysis.
abstract: Phenolic compounds belong to a class of secondary metabolites and are implicated in a wide range of responsive mechanisms in plants triggered by both biotic and abiotic elicitors. In this study, we approached the combinational effects of ethylene and MeJA (methyl jasmonate) on phenolic compounds profiles and gene expressions in the medicinal plant Catharanthus roseus. In virtue of a widely non-targeted metabolomics method, we identified a total of 34 kinds of phenolic compounds in the leaves, composed by 7 C6C1-, 11 C6C3-, and 16 C6C3C6 compounds. In addition, 7 kinds of intermediates critical for the biosynthesis of phenolic compounds and alkaloids were identified and discussed with phenolic metabolism. The combinational actions of ethylene and MeJA effectively promoted the total phenolic compounds, especially the C6C1 compounds (such as salicylic acid, benzoic acid) and C6C3 ones (such as cinnamic acid, sinapic acid). In contrast, the C6C3C6 compounds displayed a notably inhibitory trend in this case. Subsequently, the gene-to-metabolite networks were drawn up by searching for correlations between the expression profiles of 5 gene tags and the accumulation profiles of 41 metabolite peaks. Generally, we provide an insight into the controlling mode of ethylene-MeJA combination on phenolic metabolism in C. roseus leaves.
authors.affiliation: The Key Laboratory of Plant Ecology, Northeast Forestry University Harbin, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.affiliation: The Key Laboratory of Plant Ecology, Northeast Forestry University Harbin, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: The Key Laboratory of Plant Ecology, Northeast Forestry University Harbin, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: The Key Laboratory of Plant Ecology, Northeast Forestry University Harbin, China.
authors.forename: Zhong-Hua
authors.initials: ZH
authors.lastname: Zhang
authors.affiliation: The Key Laboratory of Plant Ecology, Northeast Forestry University Harbin, China.
authors.forename: Yuan-Gang
authors.initials: YG
authors.lastname: Zu
authors.affiliation: Department of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University Mainz, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Efferth
authors.affiliation: The Key Laboratory of Plant Ecology, Northeast Forestry University Harbin, China.
authors.forename: Zhong-Hua
authors.initials: ZH
authors.lastname: Tang
keywords: Catharanthus roseus; ethylene; methy jasmonate; non-targeted metabolomics; phenolic compounds