title: Quantitative assessment of organ distribution of dietary protein-bound13C-labeled Nɛ-carboxymethyllysine after a chronic oral exposure in mice.
abstract: Nɛ-Carboxymethyl-lysine (CML) is a prominent advanced glycation end-product which is not only found in vivo but also in food. It is known that a percentage of the dietary CML (dCML) is absorbed into the circulation and only partly excreted in the urine. Several studies have tried to measure how much dCML remains in tissues. However obstacles to interpreting the data have been found.
  
  
  
  A new protocol which discriminates dCML from native CML (nCML) has been developed. Three CML isotopes with different mass-to-charge ratios were used: nCML Nε-carboxymethyl-L-lysine, dCML Nε-[13C]carboxy[13C]methyl-L-lysine and internal standard Nε-carboxymethyl-L-[4,4,5,5-2H4]lysine. Wild-type (n = 7) and RAGE-/-(n = 8) mice were fed for 30 days with either a control, or a BSA-bound dCML-enriched diet. Organs were analyzed for nCML and dCML using liquid chromatography-tandem mass spectrometry. Mice exposed to dCML showed an accumulation in all tissues tested except fat. The rate of deposition was high (81-320 μgdCML/g dry matter) in kidneys, intestine, and lungs and low (<5 μg/g) in heart, muscle, and liver. This accumulation was not RAGE dependent.
  
  
  
  The kidney is not the only organ affected by the accumulation of dCML. Its high accumulation in other tissues and organs may also, however, have important physiological consequences.
authors.affiliation: Univ. Lille, Inserm, CHU Lille, U995 - LIRIC - Lille Inflammation Research International Center, Lille, France.|Institut Polytechnique LaSalle Beauvais, EGEAL Unit, Beauvais, France.
authors.forename: Frédéric J
authors.initials: FJ
authors.lastname: Tessier
authors.affiliation: Institut Polytechnique LaSalle Beauvais, EGEAL Unit, Beauvais, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Niquet-Léridon
authors.affiliation: Institut Polytechnique LaSalle Beauvais, EGEAL Unit, Beauvais, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Jacolot
authors.affiliation: Institut Polytechnique LaSalle Beauvais, EGEAL Unit, Beauvais, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Jouquand
authors.affiliation: Univ. Lille, EA 2694-Santé publique: épidémiologie et qualité des soins, Lille, France.
authors.forename: Michaël
authors.initials: M
authors.lastname: Genin
authors.affiliation: Diabetes Research Center, Department of Medicine, NYU Langone Medical Center, New York, NY, USA.
authors.forename: Ann-Marie
authors.initials: AM
authors.lastname: Schmidt
authors.affiliation: Univ. Lille, Inserm, CHU Lille, U995 - LIRIC - Lille Inflammation Research International Center, Lille, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Grossin
authors.affiliation: Univ. Lille, Inserm, CHU Lille, U995 - LIRIC - Lille Inflammation Research International Center, Lille, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Boulanger
mesh_terms: D000818:Animals; D004032:Diet; D004044:Dietary Proteins / Q000032:analysis / Q000378:metabolism*; D017127:Glycation End Products, Advanced / Q000378:metabolism; D006801:Humans; D007668:Kidney; D008099:Liver / Q000737:chemistry; D008239:Lysine / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D051379:Mice
chemical_list: D004044:Dietary Proteins; D017127:Glycation End Products, Advanced; C048496:N(6)-carboxymethyllysine; D008239:Lysine
keywords: Biodistribution; Carboxymethyllysine; Food; Glycation; Mass spectrometry; RAGE