title: Interleukin-10 -1082 G/A gene polymorphisms in Egyptian children with CAP: A case-control study.
abstract: Community-acquired pneumonia (CAP) is one of the leading causes of death worldwide. Cytokines are involved in the pathogenesis of CAP. To date, only a few studies concerned the association of interleukin-10 (IL-10) gene polymorphisms with CAP.In this study, we aimed to investigate whether the -1082(G/A) polymorphism in the promoter region of the IL-10 gene is involved in susceptibility to and the outcome of CAP, and we also measured the serum level of IL-10 to assess its relation to such polymorphism.This was a case-control study included 100 patients with CAP, and matched with age, gender, and ethnicity of 100 healthy control children. IL-10 -1082(G/A) gene polymorphism was genotyped by polymerase chain reaction-restriction fragment length polymorphism, while the serum IL-10 levels were measured by ELISA method.Compared to the controls subjects, the frequencies of the IL-10 -1082 AA genotype and A allele were observed to be overrepresented in patients with CAP (51%; odds ratio [OR] = 2.8; 95% confidence interval [CI]: 1.5-5.3 for the AA genotype; P < 0.01) and (70%; OR: 1.95; 95% CI: 1.27-3.00 for the A allele; P < 0.01, respectively). We found that patients with the GG genotype had significantly higher serum IL-10 levels (46.7 ± 9.5 pg/mL) compared to those with AG genotype (21.8 ± 4.5 pg/mL) and AA genotype (11.5 ± 3.3 pg/mL); P < 0.01, respectively. Our data revealed a significant positive association between the -1082 GG genotype and susceptibility to severe sepsis, acute respiratory failure, and hospital mortality (OR: 3.8; 95% CI: 1.3-11.2; P < 0.01).We demonstrate for the first time, to the best of our knowledge, that IL-10 -1082 (G/A) gene polymorphism may contribute to susceptibility to CAP in Egyptian children. Moreover, we observed that the presence of a G allele or GG genotype at the -1082 position of the promoter region of the IL-10 gene constitute risk factors for developing severe sepsis, acute respiratory failure, and hospital mortality among patients with CAP.
authors.affiliation: Faculty of Medicine, Zagazig University, Egypt.
authors.forename: Seham F
authors.initials: SF
authors.lastname: Azab
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: Abdalhady
authors.forename: Hosam F
authors.initials: HF
authors.lastname: Elsaadany
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: Elkomi
authors.forename: Eman M
authors.initials: EM
authors.lastname: Elhindawy
authors.forename: Dina T
authors.initials: DT
authors.lastname: Sarhan
authors.forename: Mohamed M A
authors.initials: MM
authors.lastname: Salam
authors.forename: Mayy A N
authors.initials: MA
authors.lastname: Allah
authors.forename: Ahmed A
authors.initials: AA
authors.lastname: Emam
authors.forename: Maha A
authors.initials: MA
authors.lastname: Noah
authors.forename: Nasser I
authors.initials: NI
authors.lastname: Abdelsalam
authors.forename: Sawsan H
authors.initials: SH
authors.lastname: Abdellatif
authors.forename: Anwar A
authors.initials: AA
authors.lastname: Rass
authors.forename: Sanaa M
authors.initials: SM
authors.lastname: Ismail
authors.forename: Tarek
authors.initials: T
authors.lastname: Gheith
authors.forename: Khalid A
authors.initials: KA
authors.lastname: Aziz
authors.forename: Mohammed E
authors.initials: ME
authors.lastname: Hamed
authors.forename: Hind M
authors.initials: HM
authors.lastname: Abdelrahman
authors.forename: Ahmed R
authors.initials: AR
authors.lastname: Ahmed
authors.forename: Rehab M
authors.initials: RM
authors.lastname: Nabil
authors.forename: Rehab S
authors.initials: RS
authors.lastname: Abdulmaksoud
authors.forename: Hala Y
authors.initials: HY
authors.lastname: Yousef
mesh_terms: D000293:Adolescent; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D017714:Community-Acquired Infections / Q000235:genetics; D004534:Egypt; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D007223:Infant; D016753:Interleukin-10 / Q000097:blood / Q000235:genetics*; D008297:Male; D018410:Pneumonia, Bacterial / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011446:Prospective Studies
chemical_list: C508609:IL10 protein, human; D016753:Interleukin-10