title: A Brachypodium UDP-Glycosyltransferase Confers Root Tolerance to Deoxynivalenol and Resistance to Fusarium Infection.
abstract: Fusarium head blight (FHB) is a cereal disease caused by Fusarium graminearum, a fungus able to produce type B trichothecenes on cereals, including deoxynivalenol (DON), which is harmful for humans and animals. Resistance to FHB is quantitative, and the mechanisms underlying resistance are poorly understood. Resistance has been related to the ability to conjugate DON into a glucosylated form, deoxynivalenol-3-O-glucose (D3G), by secondary metabolism UDP-glucosyltransferases (UGTs). However, functional analyses have never been performed within a single host species. Here, using the model cereal species Brachypodium distachyon, we show that the Bradi5g03300 UGT converts DON into D3G in planta. We present evidence that a mutation in Bradi5g03300 increases root sensitivity to DON and the susceptibility of spikes to F. graminearum, while overexpression confers increased root tolerance to the mycotoxin and spike resistance to the fungus. The dynamics of expression and conjugation suggest that the speed of DON conjugation rather than the increase of D3G per se is a critical factor explaining the higher resistance of the overexpressing lines. A detached glumes assay showed that overexpression but not mutation of the Bradi5g03300 gene alters primary infection by F. graminearum, highlighting the involvement of DON in early steps of infection. Together, these results indicate that early and efficient UGT-mediated conjugation of DON is necessary and sufficient to establish resistance to primary infection by F. graminearum and highlight a novel strategy to promote FHB resistance in cereals.
authors.affiliation: IPS2, UMR9213/UMR1403, CNRS, INRA, UPSud, UPD, SPS, 91405 Orsay, France;INRA, UMR1318, IJPB, RD10, F-78000 Versailles, France;APT, IJPB, RD10, F-78000 Versailles, France; andINRA/UR1264 MycSA, Domaine de la Grande-Ferrade CS20032, 33883 Villenave d'Ornon cedex, France.
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Pasquet
authors.affiliation: IPS2, UMR9213/UMR1403, CNRS, INRA, UPSud, UPD, SPS, 91405 Orsay, France;INRA, UMR1318, IJPB, RD10, F-78000 Versailles, France;APT, IJPB, RD10, F-78000 Versailles, France; andINRA/UR1264 MycSA, Domaine de la Grande-Ferrade CS20032, 33883 Villenave d'Ornon cedex, France.
authors.forename: Valentin
authors.identifier: 0000-0001-5152-0768
authors.initials: V
authors.lastname: Changenet
authors.affiliation: IPS2, UMR9213/UMR1403, CNRS, INRA, UPSud, UPD, SPS, 91405 Orsay, France;INRA, UMR1318, IJPB, RD10, F-78000 Versailles, France;APT, IJPB, RD10, F-78000 Versailles, France; andINRA/UR1264 MycSA, Domaine de la Grande-Ferrade CS20032, 33883 Villenave d'Ornon cedex, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Macadré
authors.affiliation: IPS2, UMR9213/UMR1403, CNRS, INRA, UPSud, UPD, SPS, 91405 Orsay, France;INRA, UMR1318, IJPB, RD10, F-78000 Versailles, France;APT, IJPB, RD10, F-78000 Versailles, France; andINRA/UR1264 MycSA, Domaine de la Grande-Ferrade CS20032, 33883 Villenave d'Ornon cedex, France.
authors.forename: Edouard
authors.identifier: 0000-0001-7605-7474
authors.initials: E
authors.lastname: Boex-Fontvieille
authors.affiliation: IPS2, UMR9213/UMR1403, CNRS, INRA, UPSud, UPD, SPS, 91405 Orsay, France;INRA, UMR1318, IJPB, RD10, F-78000 Versailles, France;APT, IJPB, RD10, F-78000 Versailles, France; andINRA/UR1264 MycSA, Domaine de la Grande-Ferrade CS20032, 33883 Villenave d'Ornon cedex, France.
authors.forename: Camille
authors.initials: C
authors.lastname: Soulhat
authors.affiliation: IPS2, UMR9213/UMR1403, CNRS, INRA, UPSud, UPD, SPS, 91405 Orsay, France;INRA, UMR1318, IJPB, RD10, F-78000 Versailles, France;APT, IJPB, RD10, F-78000 Versailles, France; andINRA/UR1264 MycSA, Domaine de la Grande-Ferrade CS20032, 33883 Villenave d'Ornon cedex, France.
authors.forename: Oumaya
authors.initials: O
authors.lastname: Bouchabké-Coussa
authors.affiliation: IPS2, UMR9213/UMR1403, CNRS, INRA, UPSud, UPD, SPS, 91405 Orsay, France;INRA, UMR1318, IJPB, RD10, F-78000 Versailles, France;APT, IJPB, RD10, F-78000 Versailles, France; andINRA/UR1264 MycSA, Domaine de la Grande-Ferrade CS20032, 33883 Villenave d'Ornon cedex, France.
authors.forename: Marion
authors.identifier: 0000-0002-1673-3095
authors.initials: M
authors.lastname: Dalmais
authors.affiliation: IPS2, UMR9213/UMR1403, CNRS, INRA, UPSud, UPD, SPS, 91405 Orsay, France;INRA, UMR1318, IJPB, RD10, F-78000 Versailles, France;APT, IJPB, RD10, F-78000 Versailles, France; andINRA/UR1264 MycSA, Domaine de la Grande-Ferrade CS20032, 33883 Villenave d'Ornon cedex, France.
authors.forename: Vessela
authors.identifier: 0000-0002-4561-5493
authors.initials: V
authors.lastname: Atanasova-Pénichon
authors.affiliation: IPS2, UMR9213/UMR1403, CNRS, INRA, UPSud, UPD, SPS, 91405 Orsay, France;INRA, UMR1318, IJPB, RD10, F-78000 Versailles, France;APT, IJPB, RD10, F-78000 Versailles, France; andINRA/UR1264 MycSA, Domaine de la Grande-Ferrade CS20032, 33883 Villenave d'Ornon cedex, France.
authors.forename: Abdelhafid
authors.initials: A
authors.lastname: Bendahmane
authors.affiliation: IPS2, UMR9213/UMR1403, CNRS, INRA, UPSud, UPD, SPS, 91405 Orsay, France;INRA, UMR1318, IJPB, RD10, F-78000 Versailles, France;APT, IJPB, RD10, F-78000 Versailles, France; andINRA/UR1264 MycSA, Domaine de la Grande-Ferrade CS20032, 33883 Villenave d'Ornon cedex, France.
authors.forename: Patrick
authors.identifier: 0000-0002-4155-1314
authors.initials: P
authors.lastname: Saindrenan
authors.affiliation: IPS2, UMR9213/UMR1403, CNRS, INRA, UPSud, UPD, SPS, 91405 Orsay, France;INRA, UMR1318, IJPB, RD10, F-78000 Versailles, France;APT, IJPB, RD10, F-78000 Versailles, France; andINRA/UR1264 MycSA, Domaine de la Grande-Ferrade CS20032, 33883 Villenave d'Ornon cedex, France marie.dufresne@u-psud.fr.
authors.forename: Marie
authors.initials: M
authors.lastname: Dufresne
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D058431:Brachypodium / Q000201:enzymology / Q000235:genetics*; D060467:Disease Resistance / Q000235:genetics; D005670:Fusarium / Q000378:metabolism / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D005960:Glucosides / Q000378:metabolism; D016695:Glycosyltransferases / Q000235:genetics* / Q000378:metabolism; D054884:Host-Pathogen Interactions; D007700:Kinetics; D009154:Mutation; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000201:enzymology / Q000235:genetics* / Q000382:microbiology; D030821:Plants, Genetically Modified; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014255:Trichothecenes / Q000378:metabolism; D014530:Uridine Diphosphate / Q000378:metabolism
chemical_list: D005960:Glucosides; D010940:Plant Proteins; D014255:Trichothecenes; C542050:deoxynivalenol-3-glucoside; D014530:Uridine Diphosphate; D016695:Glycosyltransferases; C007262:deoxynivalenol