title: D2 Region of the 28S RNA Gene: A Too-Conserved Fragment for Inferences on Phylogeny of South American Triatomines.
abstract: The brasiliensis complex is composed of five triatomine species, and different approaches suggest that Triatoma lenti and Triatoma petrochiae may be the new members. Therefore, this study sought to analyze the phylogenetic relationships within this complex by means of the D2 region of the 28S RNA gene, and to analyze the degree of polymorphism and phylogenetic significance of this gene for South American triatomines. Phylogenetic analysis by using sequence fragments of the D2 domain did not allow to perform phylogenetic inferences on species within the brasiliensis complex, because the gene alignment composed of a matrix with 37 specimens exhibited only two variable sites along the 567 base pairs used. Furthermore, if all South American species are included, only four variable sites were detected, reflecting the high degree of gene conservation. Therefore, we do not recommend the use of this gene for phylogenetic reconstruction for this group of Chagas disease vectors.
authors.affiliation: Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista, São José do Rio Preto, Sao Paulo, Brazil.
authors.forename: Ana Letícia
authors.initials: AL
authors.lastname: Guerra
authors.affiliation: Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista, São José do Rio Preto, Sao Paulo, Brazil. kaiochaboli@hotmail.com.
authors.forename: Kaio Cesar Chaboli
authors.initials: KC
authors.lastname: Alevi
authors.affiliation: Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista, São José do Rio Preto, Sao Paulo, Brazil.
authors.forename: Cecília Artico
authors.initials: CA
authors.lastname: Banho
authors.affiliation: Departamento de Ciências Biológicas, Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista, Araraquara, Sao Paulo, Brazil.
authors.forename: Jader
authors.initials: J
authors.lastname: de Oliveira
authors.affiliation: Departamento de Ciências Biológicas, Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista, Araraquara, Sao Paulo, Brazil.
authors.forename: João Aristeu
authors.initials: JA
authors.lastname: da Rosa
authors.affiliation: Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista, São José do Rio Preto, Sao Paulo, Brazil.
authors.forename: Maria Tercília
authors.initials: MT
authors.lastname: Vilela de Azeredo-Oliveira
mesh_terms: D017124:Conserved Sequence / Q000235:genetics*; D010802:Phylogeny; D012339:RNA, Ribosomal, 28S / Q000235:genetics*; D013020:South America; D014345:Trypanosoma / Q000235:genetics*
chemical_list: D012339:RNA, Ribosomal, 28S