title: Intramolecular diffusion controls aggregation of the PAPf39 peptide.
abstract: The 39-residue fragment of human prostatic acidic phosphatase (PAP) is found in high concentrations in semen and easily form fibrils. Previous work has shown that fibrillization is accelerated with a deletion of the first 8, mostly charged residues and it was hypothesized that fibrillization depended on the dynamics of these peptides. To test this hypothesis we have measured the intramolecular diffusion of the full length and 8-residue deletion peptides at two different pHs and found a correlation with fibrillization lag time. These results can be explained by a simple kinetic model of the early stages of aggregation in which oligomerization is controlled by the rate of peptide reconfiguration.
authors.affiliation: Department of Physics and Astronomy, Michigan State University, East Lansing, MI 48824, USA.
authors.forename: Kinshuk R
authors.initials: KR
authors.lastname: Srivastava
authors.affiliation: Department of Biology and Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, USA.
authors.forename: Kinsley C
authors.initials: KC
authors.lastname: French
authors.affiliation: Department of Biology and Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, USA.
authors.forename: Franco O
authors.initials: FO
authors.lastname: Tzul
authors.affiliation: Department of Biology and Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180, USA. Electronic address: makhag@rpi.edu.
authors.forename: George I
authors.initials: GI
authors.lastname: Makhatadze
authors.affiliation: Department of Physics and Astronomy, Michigan State University, East Lansing, MI 48824, USA; Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824, USA. Electronic address: lapidus@pa.msu.edu.
authors.forename: Lisa J
authors.initials: LJ
authors.lastname: Lapidus
mesh_terms: D000135:Acid Phosphatase / Q000737:chemistry*; D000682:Amyloid / Q000737:chemistry; D004058:Diffusion; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010446:Peptide Fragments / Q000737:chemistry*; D066329:Protein Aggregates
chemical_list: D000682:Amyloid; D010446:Peptide Fragments; D066329:Protein Aggregates; D000135:Acid Phosphatase; C428439:prostatic acid phosphatase