title: Anti-inflammatory effect of conditioned medium from human uterine cervical stem cells in uveitis.
abstract: The aim of the present study was to evaluate the effect of conditioned medium from human uterine cervical stem cells (CM-hUCESCs) in uveitis. To do that, uveitis was induced in rats after footpad injection of Escherichia coli lipopolysaccaride (LPS). Human retinal pigment epithelial (ARPE-19) cells after LPS challenge were used to test anti-inflammatory effect of CM-hUCESCs 'ìn vitro'. Real-time PCR was used to evaluate mRNA expression levels of the pro-inflammatory cytokines interkeukin-6, interkeukin-8, macrophage inflammatory protein-1 alpha, tumor necrosis factor alpha, and the anti-inflammatory interkeukin-10. Leucocytes from aqueous humor (AqH) were quantified in a Neubauer chamber, and eye histopathological analysis was done with hematoxylin-eosin staining. Additionally, using a human cytokine antibody array we evaluated CM-hUCESCs to determine mediating proteins. Results showed that administration of CM-hUCESCs significantly reduced LPS-induced pro-inflammatory cytokines both 'in vitro' and 'in vivo', and decreased leucocytes in AqH and ocular tissues. High levels of cytokines with anti-inflammatory effects were found in CM-hUCESCs, suggesting a possible role of these factors in reducing intraocular inflammation. In summary, treatment with CM-hUCESCs significantly reduces inflammation in uveitis. Our data indicate that CM-hUCESCs could be regarded as a potential therapeutic agent for patients suffering from ocular inflammation.
authors.affiliation: Department of Physiology-CIMUS, University of Santiago de Compostela, 15782, Santiago de Compostela, Spain. Electronic address: maria.alvarez.bermudez@udc.es.
authors.forename: Maria A
authors.initials: MA
authors.lastname: Bermudez
authors.affiliation: Department of Physiology-CIMUS, University of Santiago de Compostela, 15782, Santiago de Compostela, Spain. Electronic address: bautistax@hotmail.com.
authors.forename: Juan
authors.initials: J
authors.lastname: Sendon-Lago
authors.affiliation: Department of Physiology-CIMUS, University of Santiago de Compostela, 15782, Santiago de Compostela, Spain. Electronic address: samuel.seoane@usc.es.
authors.forename: Samuel
authors.initials: S
authors.lastname: Seoane
authors.affiliation: Unidad de Investigación, Fundación Hospital de Jove, 33290, Gijón, Spain; Fundación para la Investigación con Células Madre Uterinas (FICEMU), 33212, Gijón, Spain. Electronic address: investigacion@hospitaldejove.com.
authors.forename: Noemi
authors.initials: N
authors.lastname: Eiro
authors.affiliation: Department of Surgery-CIMUS, University of Santiago de Compostela, and Service of Ophthalmology-IDIS, Complejo Hospitalario Universitario de Santiago de Compostela, 15782, Santiago de Compostela, Spain. Electronic address: francisco.gonzalez@usc.es.
authors.forename: Francisco
authors.initials: F
authors.lastname: Gonzalez
authors.affiliation: Unidad de Investigación, Fundación Hospital de Jove, 33290, Gijón, Spain; Fundación para la Investigación con Células Madre Uterinas (FICEMU), 33212, Gijón, Spain; Service of Ophthalmology, Fundación Hospital de Jove, 33290, Gijón, Spain. Electronic address: jorgeasaa@gmail.com.
authors.forename: Jorge
authors.initials: J
authors.lastname: Saa
authors.affiliation: Unidad de Investigación, Fundación Hospital de Jove, 33290, Gijón, Spain; Fundación para la Investigación con Células Madre Uterinas (FICEMU), 33212, Gijón, Spain. Electronic address: franvizoso@gmail.com.
authors.forename: Francisco
authors.initials: F
authors.lastname: Vizoso
authors.affiliation: Department of Physiology-CIMUS, University of Santiago de Compostela, 15782, Santiago de Compostela, Spain. Electronic address: roman.perez.fernandez@usc.es.
authors.forename: Roman
authors.initials: R
authors.lastname: Perez-Fernandez
mesh_terms: D000818:Animals; D002452:Cell Count; D002478:Cells, Cultured; D002584:Cervix Uteri / Q000166:cytology*; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D008297:Male; D012313:RNA / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D014605:Uveitis / Q000378:metabolism / Q000473:pathology / Q000628:therapy*
chemical_list: D017077:Culture Media, Conditioned; D016207:Cytokines; D012313:RNA
keywords: ARPE-19; Endotoxin induced uveitis; Ocular inflammation; Secretoma; Uveitis