title: Increased cell proliferation and neural activity by physostigmine in the telencephalon of adult zebrafish.
abstract: Physostigmine, an acetylcholinesterase inhibitor, is known to affect the brain function in various aspects. This study was conducted to test whether physostigmine affects cell proliferation in the telencephalon of zebrafish. BrdU-labeled cells was prominently observed in the ventral zone of the ventral telencephalon of zebrafish. The increased number of BrdU- and proliferating cell nuclear antigen-labeled cells were shown in zebrafish treated with 200μM physostigmine, which was inhibited by pretreatment with 200μM scopolamine. iNOS mRNA expression was increased in the brain of zebrafish treated with 200μM physostigmine. Consistently, aminoguanidine, an iNOS inhibitor, attenuated the increase in the number of BrdU-labeled cells by physostigmine treatment. Zebrafish also showed seizure-like locomotor activity characterized by a rapid and abrupt movement during a 30min treatment with 200μM physostigmine. Neural activity in response to an electrical stimulus was increased in the isolated telencephalon of zebrafish continuously perfused with 200μM physostigmine. None of the number of BrdU-labeled cells, neural activity, or locomotor activity was affected by treatment with 20μM physostigmine. These results suggest that 200μM physostigmine increased neural activity and induced cell proliferation via nitric oxide production in zebrafish.
authors.affiliation: Department of Biological Sciences, Inha University, Incheon, South Korea.
authors.forename: Yunkyoung
authors.initials: Y
authors.lastname: Lee
authors.affiliation: Department of Biological Sciences, Inha University, Incheon, South Korea.
authors.forename: Bongkyu
authors.initials: B
authors.lastname: Lee
authors.affiliation: Department of Biological Sciences, Inha University, Incheon, South Korea.
authors.forename: Sumin
authors.initials: S
authors.lastname: Jeong
authors.affiliation: Department of Biological Sciences, Inha University, Incheon, South Korea.
authors.forename: Ji-Won
authors.initials: JW
authors.lastname: Park
authors.affiliation: Department of Biological Sciences, Inha University, Incheon, South Korea.
authors.forename: Inn-Oc
authors.initials: IO
authors.lastname: Han
authors.affiliation: Department of Biological Sciences, Inha University, Incheon, South Korea. Electronic address: changlee@inha.ac.kr.
authors.forename: Chang-Joong
authors.initials: CJ
authors.lastname: Lee
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002800:Cholinesterase Inhibitors / Q000008:administration & dosage*; D029941:Fish Proteins / Q000378:metabolism; D006146:Guanidines / Q000008:administration & dosage; D008124:Locomotion / Q000187:drug effects; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors / Q000378:metabolism; D010830:Physostigmine / Q000008:administration & dosage*; D012333:RNA, Messenger / Q000378:metabolism; D013687:Telencephalon / Q000187:drug effects* / Q000201:enzymology / Q000502:physiology*; D015027:Zebrafish
chemical_list: D002800:Cholinesterase Inhibitors; D029941:Fish Proteins; D006146:Guanidines; D012333:RNA, Messenger; D010830:Physostigmine; D052247:Nitric Oxide Synthase Type II; C004479:pimagedine
keywords: Cell proliferation; Neural activity; Nitric oxide; Physostigmine; Zebrafish