title: A New Chemiluminescent Method for Evaluation of the Functional Activity of Neutrophils in Patients with Type 2 Diabetes Mellitus.
abstract: Functional activity of neutrophils was evaluated by the chemiluminescent method with successive double stimulation by soluble stimuli with different mechanisms of action: phorbol-12-myristate-13-acetate (PMA) and phormyl-methionyl-leucyl-phenilalanine (fMLP). The study was carried out in 26 patients receiving oral sugar-reducing therapy. In addition to the functional activity of neutrophils, the levels of TBA reactive products, inflammation markers, blood clotting values, and biochemical parameters were measured. The results showed mainly reduction of the granulocytic component of the immune system in the patients.
authors.affiliation: Faculty of Fundamental Medicine, M. V. Lomonosov Moscow State University, Moscow, Russia. proskurnina@gmail.com.
authors.forename: E V
authors.initials: EV
authors.lastname: Proskurnina
authors.affiliation: Faculty of Fundamental Medicine, M. V. Lomonosov Moscow State University, Moscow, Russia.
authors.forename: M M
authors.initials: MM
authors.lastname: Sozarukova
authors.affiliation: Faculty of Fundamental Medicine, M. V. Lomonosov Moscow State University, Moscow, Russia.
authors.forename: A M
authors.initials: AM
authors.lastname: Polimova
authors.affiliation: Russian Medical Academy of Postgraduate Education, Moscow, Russia.
authors.forename: M A
authors.initials: MA
authors.lastname: Prudnikova
authors.affiliation: Russian Medical Academy of Postgraduate Education, Moscow, Russia.
authors.forename: A S
authors.initials: AS
authors.lastname: Ametov
authors.affiliation: Faculty of Fundamental Medicine, M. V. Lomonosov Moscow State University, Moscow, Russia.
authors.forename: Yu A
authors.initials: YA
authors.lastname: Vladimirov
mesh_terms: D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000000981:diagnostic imaging* / Q000276:immunology; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D008163:Luminescent Measurements; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000276:immunology*
keywords: kinetic chemiluminescence; neutrophil activity; oxidative stress; type 2 diabetes Mellitus