title: Increased copper bioremediation ability of new transgenic and adapted Saccharomyces cerevisiae strains.
abstract: Environmental pollution with heavy metals is a very serious ecological problem, which can be solved by bioremediation of metal ions by microorganisms. Yeast cells, especially Saccharomyces cerevisiae, are known to exhibit a good natural ability to remove heavy metal ions from an aqueous phase. In the present work, an attempt was made to increase the copper-binding properties of S. cerevisiae. For this purpose, new strains of S. cerevisiae were produced by construction and integration of recombinant human MT2 and GFP-hMT2 genes into yeast cells. The ySA4001 strain expressed GFP-hMT2p under the constitutive pADH1 promoter and the ySA4002 and ySA4003 strains expressed hMT2 and GFP-hMT2 under the inducible pCUP1 promoter. An additional yMNWTA01 strain was obtained by adaptation of the BY4743 wild type S. cerevisiae strain to high copper concentrations. The yMNWTA01, ySA4002, and ySA4003 strains exhibited an enhanced ability for copper ion bioremediation.
authors.affiliation: Department of Chemical Engineering, Biotechnology and Materials, Ariel University, Ariel, Israel.|Department of Molecular Biology, Ariel University, Ariel, Israel.
authors.forename: Polina
authors.initials: P
authors.lastname: Geva
authors.affiliation: Department of Chemical Engineering, Biotechnology and Materials, Ariel University, Ariel, Israel.|Department of Molecular Biology, Ariel University, Ariel, Israel.
authors.forename: Rotem
authors.initials: R
authors.lastname: Kahta
authors.affiliation: Department of Chemical Engineering, Biotechnology and Materials, Ariel University, Ariel, Israel.
authors.forename: Faina
authors.initials: F
authors.lastname: Nakonechny
authors.affiliation: Department of Molecular Biology, Ariel University, Ariel, Israel.
authors.forename: Stella
authors.initials: S
authors.lastname: Aronov
authors.affiliation: Department of Chemical Engineering, Biotechnology and Materials, Ariel University, Ariel, Israel. marinan@ariel.ac.il.
authors.forename: Marina
authors.identifier: http://orcid.org/0000-0001-8493-713X
authors.initials: M
authors.lastname: Nisnevitch
mesh_terms: D001673:Biodegradation, Environmental; D003300:Copper / Q000737:chemistry / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D006801:Humans; D053517:Matrix Metalloproteinase 15 / Q000235:genetics / Q000378:metabolism*; D008668:Metallothionein / Q000378:metabolism; D019216:Metals, Heavy; D030781:Organisms, Genetically Modified; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D019216:Metals, Heavy; D003300:Copper; D008668:Metallothionein; D053517:Matrix Metalloproteinase 15
keywords: Bioremediation; Cell adaptation; Copper ions; Human metallothionein MT2; Saccharomyces cerevisiae