title: Comparative analysis of gene expression profiles of hip articular cartilage between non-traumatic necrosis and osteoarthritis.
abstract: Hip cartilage destruction is consistently observed in the non-traumatic osteonecrosis of femoral head (NOFH) and accelerates its bone necrosis. The molecular mechanism underlying the cartilage damage of NOFH remains elusive. In this study, we conducted a systematically comparative study of gene expression profiles between NOFH and osteoarthritis (OA). Hip articular cartilage specimens were collected from 12 NOFH patients and 12 controls with traumatic femoral neck fracture for microarray (n=4) and quantitative real-time PCR validation experiments (n=8). Gene expression profiling of articular cartilage was performed using Agilent Human 4×44K Microarray chip. The accuracy of microarray experiment was further validated by qRT-PCR. Gene expression results of OA hip cartilage were derived from previously published study. Significance Analysis of Microarrays (SAM) software was applied for identifying differently expressed genes. Gene ontology (GO) and pathway enrichment analysis were conducted by Gene Set Enrichment Analysis software and DAVID tool, respectively. Totally, 27 differently expressed genes were identified for NOFH. Comparing the gene expression profiles of NOFH cartilage and OA cartilage detected 8 common differently expressed genes, including COL5A1, OGN, ANGPTL4, CRIP1, NFIL3, METRNL, ID2 and STEAP1. GO comparative analysis identified 10 common significant GO terms, mainly implicated in apoptosis and development process. Pathway comparative analysis observed that ECM-receptor interaction pathway and focal adhesion pathway were enriched in the differently expressed genes of both NOFH and hip OA. In conclusion, we identified a set of differently expressed genes, GO and pathways for NOFH articular destruction, some of which were also involved in the hip OA. Our study results may help to reveal the pathogenetic similarities and differences of cartilage damage of NOFH and hip OA.
authors.affiliation: Key Laboratory of Traece Elements and Endemic Diseases of National Health and Family Planning Commission, School of Public Health, Health Science Center, Xi'an Jiaotong University, PR China.
authors.forename: Wenyu
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Rheumatology, Xi'an Fifth Hospital, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Traece Elements and Endemic Diseases of National Health and Family Planning Commission, School of Public Health, Health Science Center, Xi'an Jiaotong University, PR China.
authors.forename: Jingcan
authors.initials: J
authors.lastname: Hao
authors.affiliation: Department of Radiation Oncology, First Affiliated Hospital, Health Science Center, Xi'an Jiaotong University, PR China.
authors.forename: Shuyu
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Traece Elements and Endemic Diseases of National Health and Family Planning Commission, School of Public Health, Health Science Center, Xi'an Jiaotong University, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wen
authors.affiliation: Key Laboratory of Traece Elements and Endemic Diseases of National Health and Family Planning Commission, School of Public Health, Health Science Center, Xi'an Jiaotong University, PR China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Xiao
authors.affiliation: Key Laboratory of Traece Elements and Endemic Diseases of National Health and Family Planning Commission, School of Public Health, Health Science Center, Xi'an Jiaotong University, PR China.
authors.forename: Awen
authors.initials: A
authors.lastname: He
authors.affiliation: Key Laboratory of Traece Elements and Endemic Diseases of National Health and Family Planning Commission, School of Public Health, Health Science Center, Xi'an Jiaotong University, PR China.
authors.forename: Qianrui
authors.initials: Q
authors.lastname: Fan
authors.affiliation: Key Laboratory of Traece Elements and Endemic Diseases of National Health and Family Planning Commission, School of Public Health, Health Science Center, Xi'an Jiaotong University, PR China. Electronic address: fzhxjtu@mail.xjtu.edu.cn.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, Second Affiliated Hospital of Health Science Center of Xi'an Jiaotong University, Xi'an, Shaanxi, PR China. Electronic address: liuryu@126.com.
authors.forename: Ruiyu
authors.initials: R
authors.lastname: Liu
mesh_terms: D000328:Adult; D002358:Cartilage, Articular / Q000473:pathology*; D016022:Case-Control Studies; D005260:Female; D005271:Femur Head Necrosis / Q000235:genetics*; D020869:Gene Expression Profiling; D063990:Gene Ontology; D006615:Hip / Q000473:pathology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D015207:Osteoarthritis, Hip / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results
keywords: Cartilage; Gene expression profile; Hip osteoarthritis; Osteonecrosis of femoral head