title: Biomimetic mineralization of recombinant collagen type I derived protein to obtain hybrid matrices for bone regeneration.
abstract: Understanding the mineralization mechanism of synthetic protein has recently aroused great interest especially in the development of advanced materials for bone regeneration. Herein, we propose the synthesis of composite materials through the mineralization of a recombinant collagen type I derived protein (RCP) enriched with RGD sequences in the presence of magnesium ions (Mg) to closer mimic bone composition. The role of both RCP and Mg ions in controlling the precipitation of the mineral phase is in depth evaluated. TEM and X-ray powder diffraction reveal the crystallization of nanocrystalline apatite (Ap) in all the evaluated conditions. However, Raman spectra point out also the precipitation of amorphous calcium phosphate (ACP). This amorphous phase is more evident when RCP and Mg are at work, indicating the synergistic role of both in stabilizing the amorphous precursor. In addition, hybrid matrices are prepared to tentatively address their effectiveness as scaffolds for bone tissue engineering. SEM and AFM imaging show an homogeneous mineral distribution on the RCP matrix mineralized in presence of Mg, which provides a surface roughness similar to that found in bone. Preliminary in vitro tests with pre-osteoblast cell line show good cell-material interaction on the matrices prepared in the presence of Mg. To the best of our knowledge this work represents the first attempt to mineralize recombinant collagen type I derived protein proving the simultaneous effect of the organic phase (RCP) and Mg on ACP stabilization. This study opens the possibility to engineer, through biomineralization process, advanced hybrid matrices for bone regeneration.
authors.affiliation: Institute of Science and Technology for Ceramics (ISTEC), National Research Council (CNR), Faenza, Italy. Electronic address: gloria.rodriguez@istec.cnr.it.
authors.forename: Gloria Belén
authors.initials: GB
authors.lastname: Ramírez-Rodríguez
authors.affiliation: Instituto Andaluz de Ciencias de la Tierra, IACT (CSIC-UGR), Granada, Spain.
authors.forename: José Manuel
authors.initials: JM
authors.lastname: Delgado-López
authors.affiliation: Institute of Science and Technology for Ceramics (ISTEC), National Research Council (CNR), Faenza, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Iafisco
authors.affiliation: Institute of Science and Technology for Ceramics (ISTEC), National Research Council (CNR), Faenza, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Montesi
authors.affiliation: Institute of Science and Technology for Ceramics (ISTEC), National Research Council (CNR), Faenza, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Sandri
authors.affiliation: Institute of Science and Technology for Ceramics (ISTEC), National Research Council (CNR), Faenza, Italy.
authors.forename: Simone
authors.initials: S
authors.lastname: Sprio
authors.affiliation: Institute of Science and Technology for Ceramics (ISTEC), National Research Council (CNR), Faenza, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Tampieri
mesh_terms: D000818:Animals; D001031:Apatites; D032701:Biomimetics / Q000379:methods; D001861:Bone Regeneration; D002113:Calcification, Physiologic; D002460:Cell Line; D024042:Collagen Type I / Q000378:metabolism; D008274:Magnesium; D051379:Mice; D008903:Minerals; D015202:Protein Engineering; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001031:Apatites; D024042:Collagen Type I; D008903:Minerals; D008274:Magnesium
keywords: Biomineralization; Bone; Hydroxyapatite; Magnesium; Recombinant collagen derived protein