title: PLGA nanoparticles for ocular delivery of loteprednol etabonate: a corneal penetration study.
abstract: The purpose of the present study was to develop loteprednol etabonate (LE) loaded poly(d,l-lactide co-glycolide) (PLGA) nanoparticles (NPs) and study their penetration profile into the excised goat cornea. In the present study, LE loaded PLGA NPs were prepared by solvent evaporation with high speed homogenization method and the penetration profile was studied using confocal laser scanning microscopy (CLSM). Rhodamine (Rd) was used as a fluorescent marker to prepare Rd-LE-PLGA-NPs. The NPs were characterized for particle size, X-ray diffraction (XRD), differential scanning calorimetry (DSC), transmission electron microscopy (TEM), drug entrapment, and permeation profile. Intense fluorescence observed across the depths of goat corneal tissue suggested an improved penetration profile of NPs. The entrapment efficiency and mean diameter of the optimized formulation (F5) were found to be 96.31 ± 1.68% and 167.6 ± 0.37 nm, respectively. These findings indicate that LE loaded PLGA NPs may serve as a potential drug carrier for ocular administration in eye disease.
authors.affiliation: a University Institute of Pharmacy, Faculty of Technology, Pt. Ravishankar Shukla University , Raipur , Chhattisgarh , India.
authors.forename: Abhishek K
authors.initials: AK
authors.lastname: Sah
authors.affiliation: a University Institute of Pharmacy, Faculty of Technology, Pt. Ravishankar Shukla University , Raipur , Chhattisgarh , India.
authors.forename: Preeti K
authors.initials: PK
authors.lastname: Suresh
authors.affiliation: b State Drug Testing Laboratory, Food and Drugs Administration , Govt. of Chhattisgarh , Raipur , Chhattisgarh , India.
authors.forename: Vinod K
authors.initials: VK
authors.lastname: Verma
mesh_terms: D060433:Administration, Ophthalmic; D000818:Animals; D003315:Cornea / Q000378:metabolism*; D016503:Drug Delivery Systems / Q000379:methods*; D005128:Eye Diseases / Q000188:drug therapy / Q000378:metabolism; D006041:Goats; D019344:Lactic Acid / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D000069559:Loteprednol Etabonate / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D053758:Nanoparticles / Q000737:chemistry / Q000627:therapeutic use; D010539:Permeability; D011100:Polyglycolic Acid / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer
chemical_list: D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D000069559:Loteprednol Etabonate
keywords: CLSM; PLGA; corneal penetration; loteprednol etabonate; nanoparticle; ocular delivery