title: Syntaxin 5 Overexpression and β-Amyloid 1-42 Accumulation in Endoplasmic Reticulum of Hippocampal Cells in Rat Brain Induced by Ozone Exposure.
abstract: Oxidative stress is a risk factor for Alzheimer's disease and it is currently accepted that oxidative damage precedes the overproduction of A42 peptide. We have reported that ozone causes oxidative stress inducing neurodegeneration in the brain of rats. It is associated with A42 overproduction and intracellular accumulation in hippocampus. Organelles like mitochondria, intracellular membranes, and endoplasmic reticulum have been identified as sites of A42 production and accumulation affecting cellular metabolism. However whether ozone exposure induces overproduction and/or accumulation of A42 in endoplasmic reticulum has not been studied. We evaluated this effect in the endoplasmic reticulum of hippocampal cells of rats exposed chronically to low doses of ozone (0.25 ppm) at 7, 15, 30, 60, and 90 days. The effect of the presence of A42 in endoplasmic reticulum was analyzed evaluating the expression of the chaperone Syntaxin 5. Our results show an accumulation of A42 peptide in this organelle. It was observed by immunofluorescence and by WB in endoplasmic fractions from hippocampal cells of rats at 60 and 90 days of treatment. Significant overexpression of the chaperone Syntaxin 5 at 60 and 90 days of treatment was observed ((⁎) P < 0.05). These results indicate that the exposure to environmental pollutants could be involved as a risk factor for neurodegenerative processes.
authors.affiliation: Departamento de Fisiología, Facultad de Medicina, Universidad Nacional Autónoma de México, 04510 México, DF, Mexico.
authors.forename: Luis Fernando
authors.initials: LF
authors.lastname: Hernández-Zimbrón
authors.affiliation: Departamento de Fisiología, Facultad de Medicina, Universidad Nacional Autónoma de México, 04510 México, DF, Mexico.
authors.forename: Selva
authors.identifier: 0000-0002-5204-1416
authors.initials: S
authors.lastname: Rivas-Arancibia
mesh_terms: D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D018891:Dentate Gyrus / Q000187:drug effects / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D059865:Endoplasmic Reticulum Stress; D005786:Gene Expression Regulation; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008856:Microscopy, Fluorescence; D018832:Molecular Chaperones; D019636:Neurodegenerative Diseases / Q000378:metabolism; D018384:Oxidative Stress; D010126:Ozone / Q000009:adverse effects*; D010446:Peptide Fragments / Q000378:metabolism*; D050765:Qa-SNARE Proteins / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012307:Risk Factors; D013997:Time Factors
chemical_list: D016229:Amyloid beta-Peptides; D018832:Molecular Chaperones; D010446:Peptide Fragments; D050765:Qa-SNARE Proteins; C075222:amyloid beta-protein (1-42); D010126:Ozone