title: Antioxidant activities of crude phlorotannins from Sargassum hemiphyllum.
abstract: Brown algae are well known as a source of biologically active compounds, especially those having antioxidant activities, such as phlorotannins. In this study we examined the antioxidant activities of crude phlorotannins extracts (CPEs) obtained from Sargassum hemiphyllum (SH) and fractionated according to the molecular weights. When CPEs were administrated at a dose of 30 mg/kg to Kunming mice pre-treated with carbon tetrachloride (CCl4), the levels of oxidative stress indicators in the liver, kidney and brain were significantly reduced in vivo. All the components of various molecular weight fractions of CPEs exhibited greater scavenging capacities in clearing hydroxyl free radical and superoxide anion than the positive controls gallic acid, vitamin C and vitamin E. Particularly, the components greater than 30 kD obtained from ethyl acetate phase showed the highest antioxidant capacities. These results indicated that SH is a potential source for extracting phlorotannins, the algal antioxidant compounds.
authors.affiliation: Department of Food and Biology, Guangdong University of Technology, Guangzhou, 510006, China.|People's Hospital of Longhua District, Shenzhen, 518000, China.
authors.forename: Zhi-Li
authors.initials: ZL
authors.lastname: Zhao
authors.affiliation: School of Pharmacy, Zhanjiang, 524023, China.
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Yang
authors.affiliation: School of Pharmacy, Zhanjiang, 524023, China.|Shenzhen Enhance Technology Co., Ltd., Shenzhen, 518000, China.
authors.forename: Zhong-Qing
authors.initials: ZQ
authors.lastname: Gong
authors.affiliation: School of Pharmacy, Zhanjiang, 524023, China.
authors.forename: Ming-Zhu
authors.initials: MZ
authors.lastname: Pan
authors.affiliation: Department of Food and Biology, Guangdong University of Technology, Guangzhou, 510006, China. zaozao7@163.com.
authors.forename: Ya-Li
authors.initials: YL
authors.lastname: Han
authors.affiliation: Key Laboratory of Research & Development of Nature Drug, Guangdong Medical College, Zhanjiang, 524023, China. plliu78@sina.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D002251:Carbon Tetrachloride / Q000037:antagonists & inhibitors* / Q000633:toxicity; D002252:Carbon Tetrachloride Poisoning / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D005591:Chemical Fractionation / Q000379:methods; D005707:Gallic Acid / Q000494:pharmacology; D017665:Hydroxyl Radical / Q000037:antagonists & inhibitors / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D059625:Liquid-Liquid Extraction / Q000379:methods; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D000459:Phaeophyceae / Q000737:chemistry*; D044647:Sargassum / Q000737:chemistry*; D013481:Superoxides / Q000037:antagonists & inhibitors / Q000378:metabolism; D013634:Tannins / Q000302:isolation & purification / Q000494:pharmacology*; D014810:Vitamin E / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D013634:Tannins; D013481:Superoxides; D014810:Vitamin E; D017665:Hydroxyl Radical; D005707:Gallic Acid; D002251:Carbon Tetrachloride; D001205:Ascorbic Acid
keywords: Sargassum hemiphyllum; antioxidant activities; in vitro; in vivo; phlorotannins