title: Photoacoustic imaging with an acoustic lens detects prostate cancer cells labeled with PSMA-targeting near-infrared dye-conjugates.
abstract: There is an urgent need for sensitive and specific tools to accurately image early stage, organ-confined human prostate cancers to facilitate active surveillance and reduce unnecessary treatment. Recently, we developed an acoustic lens that enhances the sensitivity of photoacoustic imaging. Here, we report the use of this device in conjunction with two molecular imaging agents that specifically target the prostate-specific membrane antigen (PSMA) expressed on the tumor cell surface of most prostate cancers. We demonstrate successful imaging of phantoms containing cancer cells labeled with either of two different PSMA-targeting agents, the ribonucleic acid aptamer A10-3.2 and a urea-based peptidomimetic inhibitor, each linked to the near-infrared dye IRDye800CW. By specifically targeting cells with these agents linked to a dye chosen for optimal signal, we are able to discriminate prostate cancer cells that express PSMA.
authors.affiliation: University of Rochester, Department of Image Science, 601 Elmwood Avenue, Rochester, New York 14642, United States.
authors.forename: Vikram
authors.initials: V
authors.lastname: Dogra
authors.affiliation: University of Rochester, Department of Image Science, 601 Elmwood Avenue, Rochester, New York 14642, United States.
authors.forename: Bhargava
authors.initials: B
authors.lastname: Chinni
authors.affiliation: Roswell Park Cancer Institute, Department of Cancer Genetics, Elm and Carlton Streets, Buffalo, New York 14263, United States.
authors.forename: Shalini
authors.initials: S
authors.lastname: Singh
authors.affiliation: Rochester Institute of Technology, Carlson Center for Imaging Science, 54 Lomb Memorial Drive, Rochester, New York 14623, United StatesdRochester Institute of Technology, School of Chemistry and Materials Science, 54 Lomb Memorial Drive, Rochester, New Yo.
authors.forename: Hans
authors.initials: H
authors.lastname: Schmitthenner
authors.affiliation: Rochester Institute of Technology, Carlson Center for Imaging Science, 54 Lomb Memorial Drive, Rochester, New York 14623, United States.
authors.forename: Navalgund
authors.initials: N
authors.lastname: Rao
authors.affiliation: Roswell Park Cancer Institute, Department of Cancer Genetics, Elm and Carlton Streets, Buffalo, New York 14263, United StateseRoswell Park Cancer Institute, Center for Personalized Medicine, Elm and Carlton Streets, Buffalo, New York 14263, United States.
authors.forename: John J
authors.initials: JJ
authors.lastname: Krolewski
authors.affiliation: Roswell Park Cancer Institute, Department of Cancer Genetics, Elm and Carlton Streets, Buffalo, New York 14263, United States.
authors.forename: Kent L
authors.initials: KL
authors.lastname: Nastiuk
mesh_terms: D003952:Diagnostic Imaging / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007211:Indoles / Q000737:chemistry; D007259:Infrared Rays; D008297:Male; D061088:Photoacoustic Techniques; D017430:Prostate-Specific Antigen / Q000737:chemistry*; D011471:Prostatic Neoplasms / Q000000981:diagnostic imaging*
chemical_list: C427728:IRDye800; D007211:Indoles; D017430:Prostate-Specific Antigen