title: Platelet-rich plasma (PRP) and adipose-derived mesenchymal stem cells: stimulatory effects on proliferation and migration of fibroblasts and keratinocytes in vitro.
abstract: The clinical use of tissue engineering associated with cell therapy is considered a new alternative therapy for the repair of chronic lesions with potential application in different medical areas, mostly in orthopedic and dermatological diseases. Platelet-rich plasma (PRP) is a rich source of growth factors and cytokines important for wound healing. Adipose-derived mesenchymal stem cells (ADSCs) have shown potential to accelerate the resolution of ulcers, to stimulate cell proliferation, and to benefit the quality of skin repair. This study aims to determine the effect of PRP and conditioned medium (CM) from ADSC on fibroblast and keratinocyte proliferation in vitro. Migration and proliferation assays were performed to evaluate the growth of fibroblasts and keratinocytes in the presence of PRP, CM, and CM + PRP. Significant proliferative stimulation was observed after 48 h of culture (p < 0.05) on mean absorbance of fibroblasts cultured with 10 and 25 % PRP, 100 % CM, and 25 % PRP + 25 % CM, if compared with control. Keratinocyte proliferation was stimulated after 48 h in cultures with 25, 50, and 100 % CM, and growth was compared with controls. The migration assay detected a significant migratory stimulus in fibroblasts cultured with 10 % PRP + 10 % CM after 48 h. These in vitro results suggest that PRP and ADSC have therapeutic potential for healing and re-epithelialization of chronic wounds in vivo.
authors.affiliation: University of São Paulo, USP, São Paulo, Brazil.
authors.forename: Talita
authors.initials: T
authors.lastname: Stessuk
authors.affiliation: University of Campinas, UNICAMP, Campinas, Brazil.
authors.forename: Maria Beatriz
authors.initials: MB
authors.lastname: Puzzi
authors.affiliation: University of Campinas, UNICAMP, Campinas, Brazil.
authors.forename: Elinton Adami
authors.initials: EA
authors.lastname: Chaim
authors.affiliation: University of Campinas, UNICAMP, Campinas, Brazil.
authors.forename: Paulo César Martins
authors.initials: PC
authors.lastname: Alves
authors.affiliation: University of Campinas, UNICAMP, Campinas, Brazil.
authors.forename: Erich Vinicius
authors.initials: EV
authors.lastname: de Paula
authors.affiliation: CordCell Cell Therapy, São Paulo, Brazil.
authors.forename: Andresa
authors.initials: A
authors.lastname: Forte
authors.affiliation: University of Campinas, UNICAMP, Campinas, Brazil.
authors.forename: Juliana Massae
authors.initials: JM
authors.lastname: Izumizawa
authors.affiliation: University of Campinas, UNICAMP, Campinas, Brazil.
authors.forename: Carolina Caliári
authors.initials: CC
authors.lastname: Oliveira
authors.affiliation: São Paulo State University, UNESP, Campus de Assis, Assis, Brazil.
authors.forename: Fernando
authors.initials: F
authors.lastname: Frei
authors.affiliation: São Paulo State University, UNESP, Campus de Assis, Assis, Brazil. jtrpaes@yahoo.com.br.|Department of Biological Sciences, São Paulo State University, UNESP, Av. Dom Antonio, 2100, Assis, São Paulo, 19.806-900, Brazil. jtrpaes@yahoo.com.br.
authors.forename: João Tadeu
authors.initials: JT
authors.lastname: Ribeiro-Paes
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000328:Adult; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D064987:Cell- and Tissue-Based Therapy / Q000379:methods; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D005347:Fibroblasts / Q000502:physiology*; D006801:Humans; D015603:Keratinocytes / Q000502:physiology*; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D008875:Middle Aged; D009765:Obesity; D053657:Platelet-Rich Plasma / Q000378:metabolism*; D012867:Skin / Q000293:injuries; D023822:Tissue Engineering / Q000379:methods; D014945:Wound Healing / Q000502:physiology
chemical_list: D017077:Culture Media, Conditioned
keywords: Fibroblasts; Keratinocytes; Mesenchymal stem cells; Platelet-rich plasma