title: Understanding the response of pulsed electric field on osteoblast functions in three-dimensional mesh structures.
abstract: The endogenous electric field plays a determining role in impacting biological functions including communication with the physiological system, brain, and bone regeneration by influencing cellular functions. From this perspective, the objective of the study described here is to elucidate the effect of external electric field under dynamic conditions, in providing a guiding cue to osteoblasts in terms of cell-cell interactions and synthesis of prominent adhesion and cytoskeleton proteins. This was accomplished using pulsed direct current electric field of strength 0.1-1 V/cm. The electric field provided guided cue to the cells to migrate toward cathode. Membrane blebbing or necrosis was nearly absent in the vicinity of cathode at 0.1 and 0.5 V/cm electric field strength. Moreover, a higher cell proliferation as well as higher expression of vinculin and densely packed actin stress fibers was observed. At anode, the cells though healthy but expression of actin and vinculin was less. We underscore for the first time that the biological functionality can be favorably modulated on 3D printed scaffolds in the presence of electric field and under dynamic conditions with consequent positive effect on cell proliferation, growth, and expression level of prominent proteins.
authors.affiliation: Biomaterials and Biomedical Engineering Research Laboratory, Department of Metallurgical, Materials, and Biomedical Engineering, University of Texas at El Paso, USA.
authors.forename: A
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Biomaterials and Biomedical Engineering Research Laboratory, Department of Metallurgical, Materials, and Biomedical Engineering, University of Texas at El Paso, USA.
authors.forename: K C
authors.initials: KC
authors.lastname: Nune
authors.affiliation: Biomaterials and Biomedical Engineering Research Laboratory, Department of Metallurgical, Materials, and Biomedical Engineering, University of Texas at El Paso, USA dmisra2@utep.edu.
authors.forename: Rdk
authors.initials: R
authors.lastname: Misra
mesh_terms: D016475:3T3 Cells; D000818:Animals; D002450:Cell Communication / Q000502:physiology / Q000528:radiation effects*; D002465:Cell Movement / Q000502:physiology / Q000528:radiation effects*; D049109:Cell Proliferation / Q000502:physiology / Q000528:radiation effects*; D002470:Cell Survival / Q000502:physiology / Q000528:radiation effects; D003598:Cytoskeletal Proteins / Q000378:metabolism; D004307:Dose-Response Relationship, Radiation; D004558:Electric Stimulation / Q000379:methods*; D004574:Electromagnetic Fields; D040542:Mechanotransduction, Cellular / Q000502:physiology / Q000528:radiation effects*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology / Q000528:radiation effects*; D066330:Printing, Three-Dimensional; D011829:Radiation Dosage; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds
chemical_list: D003598:Cytoskeletal Proteins
keywords: Electric field; Ti-6Al-4V; media flow; proliferation; wound healing