title: Deriving Osteogenic Cells from Induced Pluripotent Stem Cells for Bone Tissue Engineering.
abstract: Induced pluripotent stem cells (iPSCs), reprogrammed from adult somatic cells using defined transcription factors, are regarded as a promising cell source for tissue engineering. For the purpose of bone tissue regeneration, efficient in vitro differentiation of iPSCs into downstream cells, such as mesenchymal stem cells (MSCs), osteoblasts, or osteocyte-like cells, before use is necessary to limit undesired tumorogenesis associated with the pluripotency of iPSCs. Until recently numerous techniques on the production of iPSC-derived osteogenic progenitors have been introduced. We reviewed these protocols and provided a perspective on the comparisons of osteogenic potentials of (1) iPSC-derived osteogenic cells produced by different protocols, (2) iPSCs from different somatic origins, and (3) iPSC-derived MSC-like cells and bone marrow stem cells. Finally, we discussed the potential application of the diseased iPSCs for systematic bone disorders.
authors.affiliation: 1 State Key Laboratory of Oral Diseases, Department of Oral Implantology, West China Hospital of Stomatology, Sichuan University , Chengdu, China .
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Wu
authors.affiliation: 1 State Key Laboratory of Oral Diseases, Department of Oral Implantology, West China Hospital of Stomatology, Sichuan University , Chengdu, China .
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.affiliation: 2 University of Maryland Dental School , Baltimore, Maryland.
authors.forename: Kevin
authors.initials: K
authors.lastname: Hu
authors.affiliation: 3 Department of Stomatology, China-Japan Friendship Hospital , Beijing, China .
authors.forename: Cong
authors.initials: C
authors.lastname: Cao
authors.affiliation: 1 State Key Laboratory of Oral Diseases, Department of Oral Implantology, West China Hospital of Stomatology, Sichuan University , Chengdu, China .
authors.forename: Yi
authors.initials: Y
authors.lastname: Man
authors.affiliation: 1 State Key Laboratory of Oral Diseases, Department of Oral Implantology, West China Hospital of Stomatology, Sichuan University , Chengdu, China .|2 University of Maryland Dental School , Baltimore, Maryland.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
mesh_terms: D001842:Bone and Bones; D002454:Cell Differentiation; D057026:Induced Pluripotent Stem Cells; D059630:Mesenchymal Stem Cells; D010012:Osteogenesis; D023822:Tissue Engineering
keywords: bone marrow stem cells; bone tissue engineering; induced pluripotent stem cells; osteogenic differentiation; scaffolds