title: Evaluation of antimicrobial activity and probiotic properties of wild-strain Pichia kudriavzevii isolated from frozen idli batter.
abstract: The present research was undertaken to study the probiotic characteristics of Pichia kudriavzevii isolated from frozen idli batter. Polymerase chain reaction amplification with 18S rRNA primers confirmed Pichia kudriavzevii, a xylose-utilizing probiotic strain. It was resistant to physiological concentrations of bile salts, pepsin and pancreatic enzyme. It also showed efficient auto-aggregation as well as co-aggregation ability with four commercial probiotic yeasts and exhibited good hydrophobicity in xylene and toluene. The strain inhibited the growth of 13 enteropathogens and showed a commensal relationship with four commercial probiotic yeast and bacteria. Moreover, it was resistant to 30 antibiotics with different modes of action. The yeast exhibited thermotolerance up to 95 °C for 2 h. The cell-free supernatants were also found to be heat stable, indicating the presence of thermostable secondary metabolites. Hence it could be exploited as starter culture, co-culture or probiotic in the preparation of fermented products or incorporated in heatable foods as well. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Centre for Food Technology, Department of Biotechnology, Anna University, Chennai, Tamilnadu, India.
authors.forename: Ramachandran
authors.initials: R
authors.lastname: Chelliah
authors.affiliation: Centre for Food Technology, Department of Biotechnology, Anna University, Chennai, Tamilnadu, India.
authors.forename: Sudha Rani
authors.initials: SR
authors.lastname: Ramakrishnan
authors.affiliation: Centre for Food Technology, Department of Biotechnology, Anna University, Chennai, Tamilnadu, India.
authors.forename: Prince R
authors.initials: PR
authors.lastname: Prabhu
authors.affiliation: Centre for Food Technology, Department of Biotechnology, Anna University, Chennai, Tamilnadu, India.
authors.forename: Usha
authors.initials: U
authors.lastname: Antony
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000898:Antibiosis; D001647:Bile Acids and Salts / Q000494:pharmacology; D018920:Coculture Techniques; D004352:Drug Resistance, Microbial; D005285:Fermentation; D005516:Food Microbiology; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D057927:Hydrophobic and Hydrophilic Interactions; D050296:Microbial Viability; D010641:Phenotype; D010843:Pichia / Q000187:drug effects / Q000302:isolation & purification / Q000502:physiology*; D019936:Probiotics / Q000302:isolation & purification; D014994:Xylose / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001647:Bile Acids and Salts; D014994:Xylose
keywords: Pichia kudriavzevii; idli batter; probiotics; thermoresistant; xylose