title: Dermal exposure to tannery effluent causes neurobehavioral changes in C57Bl/6J and Swiss mice.
abstract: Tannery effluents constitute highly polluting residues, which can cause negative impacts to people's health and the environment. However, studies that have investigated the effects of the exposure to these xenobiotics on the central nervous system of mammal experimental models are rare, the few that have been published focusing on the exposure via oral intake (ingestion of water containing tannery effluent concentrations). In this sense, and with the objective of expanding the knowledge beyond the neurotoxic effects observed when water contaminated by these xenobiotics is ingested, the neurobehavioral effects of dermal exposure of male C57Bl/6J and Swiss mice were analyzed. The animals were exposed to raw (wet blue-type) tannery effluent for two hours during five days, totalizing 15 days of exposure. Afterwards, the animals underwent the elevated plus-maze (predictive of anxiety) and the object recognition tests (identification of memory deficit). Our data show that the dermal exposure to the tannery effluent caused an anxiogenic behavior in these animals, when compared those that did not have direct contact with these xenobiotics. It was also observed that the animals exposed to the tannery effluent obtained lower novel object recognition indices, thus evidencing memory deficit and indicating a possible influence of the tannery effluent constituents in animal cognition. The present study attests the hypothesis that dermal exposure to tannery effluents containing neurotoxic substances causes behavioral disorders in C57Bl/6J and Swiss mice.
authors.affiliation: Programa de Pós-Graduação em Conservação dos Recursos Naturais do Cerrado, Instituto Federal Goiano - Campus Urutaí, Urutaí, GO, Brazil; Laboratório de Pesquisas Biológicas, Instituto Federal Goiano - Campus Urutaí, GO, Brazil.
authors.forename: Wellington Alves Mizael
authors.initials: WA
authors.lastname: da Silva
authors.affiliation: Laboratório de Pesquisas Biológicas, Instituto Federal Goiano - Campus Urutaí, GO, Brazil.
authors.forename: Bruna de Oliveira
authors.initials: Bde O
authors.lastname: Mendes
authors.affiliation: Programa de Pós-Graduação em Conservação dos Recursos Naturais do Cerrado, Instituto Federal Goiano - Campus Urutaí, Urutaí, GO, Brazil; Laboratório de Pesquisas Biológicas, Instituto Federal Goiano - Campus Urutaí, GO, Brazil.
authors.forename: Abraão Tiago Batista
authors.initials: AT
authors.lastname: Guimarães
authors.affiliation: Laboratório de Pesquisas Biológicas, Instituto Federal Goiano - Campus Urutaí, GO, Brazil.
authors.forename: Letícia Martins
authors.initials: LM
authors.lastname: Rabelo
authors.affiliation: Laboratório de Pesquisas Biológicas, Instituto Federal Goiano - Campus Urutaí, GO, Brazil.
authors.forename: Raíssa de Oliveira
authors.initials: Rde O
authors.lastname: Ferreira
authors.affiliation: Laboratório de Pesquisas Biológicas, Instituto Federal Goiano - Campus Urutaí, GO, Brazil.
authors.forename: Bianca Costa
authors.initials: BC
authors.lastname: E Silva
authors.affiliation: Programa de Pós-Graduação em Conservação dos Recursos Naturais do Cerrado, Instituto Federal Goiano - Campus Urutaí, Urutaí, GO, Brazil; Laboratório de Pesquisas Biológicas, Instituto Federal Goiano - Campus Urutaí, GO, Brazil.
authors.forename: Joyce Moreira
authors.initials: JM
authors.lastname: de Souza
authors.affiliation: Programa de Pós-Graduação em Conservação dos Recursos Naturais do Cerrado, Instituto Federal Goiano - Campus Urutaí, Urutaí, GO, Brazil; Laboratório de Pesquisas Biológicas, Instituto Federal Goiano - Campus Urutaí, GO, Brazil; Departamento de Ciências Biológicas, Instituto Federal Goiano - Campus Urutaí, GO, Brazil.
authors.forename: Ivandilson Pessoa Pinto
authors.initials: IP
authors.lastname: de Menezes
authors.affiliation: Programa de Pós-Graduação em Conservação dos Recursos Naturais do Cerrado, Instituto Federal Goiano - Campus Urutaí, Urutaí, GO, Brazil; Laboratório de Pesquisas Biológicas, Instituto Federal Goiano - Campus Urutaí, GO, Brazil; Departamento de Ciências Biológicas, Instituto Federal Goiano - Campus Urutaí, GO, Brazil.
authors.forename: Aline Sueli de Lima
authors.initials: AS
authors.lastname: Rodrigues
authors.affiliation: Programa de Pós-Graduação em Conservação dos Recursos Naturais do Cerrado, Instituto Federal Goiano - Campus Urutaí, Urutaí, GO, Brazil; Laboratório de Pesquisas Biológicas, Instituto Federal Goiano - Campus Urutaí, GO, Brazil; Departamento de Ciências Biológicas, Instituto Federal Goiano - Campus Urutaí, GO, Brazil; Programa de Pós-Graduação em Biodiversidade Animal, Universidade Federal de Goiás - Campus Samambaia, Goiânia, GO, Brazil. Electronic address: guilhermeifgoiano@gmail.com.
authors.forename: Guilherme
authors.initials: G
authors.lastname: Malafaia
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D001007:Anxiety / Q000139:chemically induced*; D007220:Industrial Waste / Q000009:adverse effects*; D008297:Male; D008810:Mice, Inbred C57BL; D013633:Tanning; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D007220:Industrial Waste; D014874:Water Pollutants, Chemical
keywords: C57Bl/6J; Experimental models; Mammals; Swiss mice; Tannery effluent; Xenobiotic