title: In vitro and in vivo hemocompatibility evaluation of a new dermatan sulfate-modified PET patch for vascular repair surgery.
abstract: The development of new vascular devices requires to study the effects of materials on blood cells and on coagulation, both in vitro and in vivo. In this study, we have developed a new material by grafting dermatan sulfate (DS) from shark skin onto polyethylene terephthalate (PET). We have evaluated the haemocompatibility of PET-DS material in vitro by measuring thrombin generation, plasma recalcification time, hemolytic activity, and platelet adhesion and in vivo with a model of vascular patch in rat abdominal aorta. In vitro, our results have shown that PET-DS is a nonhemolytic material, able to inhibit thrombin generation and platelet adhesion. In vivo studies by Doppler echographic evaluation 20 days after implantation have shown that the PET-DS patch was integrated in the vessel wall and covered by a layer of cells. In conclusion, PET-DS has good haemocompatibility properties and could be a promising tool for vascular surgery. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2001-2009, 2017.
authors.affiliation: Laboratoire de Pharmacologie 04/UR/01-09, Faculté de Médecine, Université de Monastir, Monastir, Tunisia.
authors.forename: Manel
authors.initials: M
authors.lastname: Dhahri
authors.affiliation: INSERM, U1148, LVTS, Université Paris 13, Université Paris Diderot, Sorbonne Paris Cité, Paris, France.
authors.forename: Violeta
authors.initials: V
authors.lastname: Rodriguez-Ruiz
authors.affiliation: INSERM, U1148, LVTS, Université Paris 13, Université Paris Diderot, Sorbonne Paris Cité, Paris, France.
authors.forename: Rachida
authors.initials: R
authors.lastname: Aid-Launais
authors.affiliation: INSERM, U1148, LVTS, Université Paris 13, Université Paris Diderot, Sorbonne Paris Cité, Paris, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Ollivier
authors.affiliation: INSERM, U1148, LVTS, Université Paris 13, Université Paris Diderot, Sorbonne Paris Cité, Paris, France.
authors.forename: Graciela
authors.initials: G
authors.lastname: Pavon-Djavid
authors.affiliation: INSERM, U1148, LVTS, Université Paris 13, Université Paris Diderot, Sorbonne Paris Cité, Paris, France.
authors.forename: Clément
authors.initials: C
authors.lastname: Journé
authors.affiliation: INSERM, U1148, LVTS, Université Paris 13, Université Paris Diderot, Sorbonne Paris Cité, Paris, France.
authors.forename: Liliane
authors.initials: L
authors.lastname: Louedec
authors.affiliation: INSERM, U1148, LVTS, Université Paris 13, Université Paris Diderot, Sorbonne Paris Cité, Paris, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Chaubet
authors.affiliation: INSERM, U1148, LVTS, Université Paris 13, Université Paris Diderot, Sorbonne Paris Cité, Paris, France.
authors.forename: Didier
authors.initials: D
authors.lastname: Letourneur
authors.affiliation: Institut Supérieur de Biotechnologie de Monastir, Laboratoire de recherche Génétique, biodiversité et valorisation des bioressources LR11ES41, Université de Monastir, Monastir, Tunisia.
authors.forename: Raoui M
authors.initials: RM
authors.lastname: Maaroufi
authors.affiliation: INSERM, U1148, LVTS, Université Paris 13, Université Paris Diderot, Sorbonne Paris Cité, Paris, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Meddahi-Pellé
mesh_terms: D000818:Animals; D001012:Aorta, Abdominal / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D001792:Blood Platelets / Q000378:metabolism*; D003871:Dermatan Sulfate / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008297:Male; D008422:Materials Testing; D010973:Platelet Adhesiveness / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D012754:Sharks; D012867:Skin / Q000737:chemistry*
chemical_list: D003871:Dermatan Sulfate
keywords: dermatan sulfate; haemocompatibility; immobilization; in vitro; in vivo; material testing; polyethylene terephthalate (PET); vascular patch