title: Nucleolin interacts with influenza A nucleoprotein and contributes to viral ribonucleoprotein complexes nuclear trafficking and efficient influenza viral replication.
abstract: Influenza viruses replicate their single-stranded RNA genomes in the nucleus of infected cells and these replicated genomes (vRNPs) are then exported from the nucleus to the cytoplasm and plasma membrane before budding. To achieve this export, influenza viruses hijack the host cell export machinery. However, the complete mechanisms underlying this hijacking remain not fully understood. We have previously shown that influenza viruses induce a marked alteration of the nucleus during the time-course of infection and notably in the nucleolar compartment. In this study, we discovered that a major nucleolar component, called nucleolin, is required for an efficient export of vRNPs and viral replication. We have notably shown that nucleolin interacts with the viral nucleoprotein (NP) that mainly constitutes vRNPs. Our results suggest that this interaction could allow vRNPs to "catch" the host cell export machinery, a necessary step for viral replication.
authors.affiliation: Virologie et Pathologie Humaine - Team VirPath - Université Claude Bernard Lyon 1 - Hospices Civils de Lyon, Lyon, France.|CIRI, International Center for Infectiology Research, Inserm U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, Lyon, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Terrier
authors.affiliation: Virologie et Pathologie Humaine - Team VirPath - Université Claude Bernard Lyon 1 - Hospices Civils de Lyon, Lyon, France.|CIRI, International Center for Infectiology Research, Inserm U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, Lyon, France.
authors.forename: Coralie
authors.initials: C
authors.lastname: Carron
authors.affiliation: CIRI, International Center for Infectiology Research, Inserm U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, Lyon, France.
authors.forename: Benoît
authors.initials: B
authors.lastname: De Chassey
authors.affiliation: Virologie et Pathologie Humaine - Team VirPath - Université Claude Bernard Lyon 1 - Hospices Civils de Lyon, Lyon, France.|CIRI, International Center for Infectiology Research, Inserm U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, Lyon, France.
authors.forename: Julia
authors.initials: J
authors.lastname: Dubois
authors.affiliation: Virologie et Pathologie Humaine - Team VirPath - Université Claude Bernard Lyon 1 - Hospices Civils de Lyon, Lyon, France.|CIRI, International Center for Infectiology Research, Inserm U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, Lyon, France.
authors.forename: Aurélien
authors.initials: A
authors.lastname: Traversier
authors.affiliation: Virologie et Pathologie Humaine - Team VirPath - Université Claude Bernard Lyon 1 - Hospices Civils de Lyon, Lyon, France.|CIRI, International Center for Infectiology Research, Inserm U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, Lyon, France.|VirNext, Faculté de Médecine RTH Laennec, Université Lyon 1, Lyon, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Julien
authors.affiliation: Virologie et Pathologie Humaine - Team VirPath - Université Claude Bernard Lyon 1 - Hospices Civils de Lyon, Lyon, France.|CIRI, International Center for Infectiology Research, Inserm U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, Lyon, France.
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Cartet
authors.affiliation: Virologie et Pathologie Humaine - Team VirPath - Université Claude Bernard Lyon 1 - Hospices Civils de Lyon, Lyon, France.|CIRI, International Center for Infectiology Research, Inserm U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, Lyon, France.|VirNext, Faculté de Médecine RTH Laennec, Université Lyon 1, Lyon, France.
authors.forename: Anaïs
authors.initials: A
authors.lastname: Proust
authors.affiliation: Centre de Recherche en Cancérologie de Lyon, UMR Inserm 1052 CNRS 5286, Centre Léon Bérard, Lyon, France and Université de Lyon, Lyon, France.
authors.forename: Sabine
authors.initials: S
authors.lastname: Hacot
authors.affiliation: CIQLE, Centre d'imagerie quantitative Lyon-Est, Université Claude Bernard Lyon 1, Lyon, France.
authors.forename: Denis
authors.initials: D
authors.lastname: Ressnikoff
authors.affiliation: CIRI, International Center for Infectiology Research, Inserm U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, Lyon, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Lotteau
authors.affiliation: Virologie et Pathologie Humaine - Team VirPath - Université Claude Bernard Lyon 1 - Hospices Civils de Lyon, Lyon, France.|CIRI, International Center for Infectiology Research, Inserm U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, Lyon, France.|Hospices Civils de Lyon, Laboratory of Virology, Lyon, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Lina
authors.affiliation: Centre de Recherche en Cancérologie de Lyon, UMR Inserm 1052 CNRS 5286, Centre Léon Bérard, Lyon, France and Université de Lyon, Lyon, France.
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Diaz
authors.affiliation: Virologie et Pathologie Humaine - Team VirPath - Université Claude Bernard Lyon 1 - Hospices Civils de Lyon, Lyon, France.|CIRI, International Center for Infectiology Research, Inserm U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, Lyon, France.|VirNext, Faculté de Médecine RTH Laennec, Université Lyon 1, Lyon, France.
authors.forename: Vincent
authors.initials: V
authors.lastname: Moules
authors.affiliation: Virologie et Pathologie Humaine - Team VirPath - Université Claude Bernard Lyon 1 - Hospices Civils de Lyon, Lyon, France.|CIRI, International Center for Infectiology Research, Inserm U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, Lyon, France.|VirNext, Faculté de Médecine RTH Laennec, Université Lyon 1, Lyon, France.
authors.forename: Manuel
authors.initials: M
authors.lastname: Rosa-Calatrava
mesh_terms: D000072283:A549 Cells; D021581:Active Transport, Cell Nucleus; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D004285:Dogs; D006801:Humans; D053122:Influenza A Virus, H3N2 Subtype / Q000502:physiology*; D061985:Madin Darby Canine Kidney Cells; D019590:Nucleocapsid Proteins; D010750:Phosphoproteins / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D014758:Viral Core Proteins / Q000378:metabolism*; D014779:Virus Replication; D000097796:Nucleolin
chemical_list: D019590:Nucleocapsid Proteins; D010750:Phosphoproteins; D016601:RNA-Binding Proteins; D014758:Viral Core Proteins; D000097796:Nucleolin; C048842:NP protein, Influenza A virus