title: Pasteurella multocida Bacteremia With Associated Knee Arthroplasty Infection in an 80-Year-Old Caucasian Man.
abstract: OBJECTIVE
  To identify the gram-negative rods grown from blood cultures and a right-knee fluid aspirate from an 80-year-old caucasian man who had undergone a total right knee arthroplastic procedure 6 years ago, and to assess the genetic similarity between the 2 isolates.
  
  
  METHODS
  We used 3 different approaches: biochemical testing, matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry, and 16S ribosomal RNA (rRNA) gene sequencing.
  
  
  RESULTS
  The 3 methods identified the gram-negative rods as Pasteurella multocida; 16S rRNA gene sequencing further identified the organisms as P. multocida subsp. septica.
  
  
  CONCLUSIONS
  A concordant identification of P. multocida was observed using biochemical testing, mass spectrometry, and 16S rRNA gene sequencing. Only 16S rRNA sequencing was able to determine the subspecies of P. multocida and to determine the genetic relatedness of the 2 isolates.
authors.affiliation: Department of Laboratory Medicine and Pathology, University of Minnesota Medical School sarbefev@umn.edu.
authors.forename: Sophie
authors.initials: S
authors.lastname: Arbefeville
authors.affiliation: Department of Laboratory Medicine and Pathology, University of Minnesota Medical School.
authors.forename: Anthony
authors.initials: A
authors.lastname: Harris
authors.affiliation: Fairview Health System, Minneapolis, MN.
authors.forename: Steven
authors.initials: S
authors.lastname: Dittes
authors.affiliation: Department of Laboratory Medicine and Pathology, University of Minnesota Medical School.
authors.forename: Patricia
authors.initials: P
authors.lastname: Ferrieri
mesh_terms: D000369:Aged, 80 and over; D019645:Arthroplasty, Replacement, Knee / Q000009:adverse effects*; D016470:Bacteremia / Q000175:diagnosis* / Q000473:pathology*; D001424:Bacterial Infections; D015373:Bacterial Typing Techniques; D001431:Bacteriological Techniques; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008297:Male; D010326:Pasteurella Infections / Q000175:diagnosis* / Q000473:pathology*; D016979:Pasteurella multocida / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D016459:Prosthesis-Related Infections / Q000150:complications*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
keywords: 16s rRNA gene; MALDI-TOF mass spectrometry; bacteremia; gram-negative coccobacilli; pasteurella multocida; prosthetic joint