title: Plasma Surface Modification of Polyhedral Oligomeric Silsequioxane-Poly(carbonate-urea) Urethane with Allylamine Enhances the Response and Osteogenic Differentiation of Adipose-Derived Stem Cells.
abstract: This study present amino functionalization of biocompatible polymer polyhedral oligomeric silsequioxane-poly(carbonate-urea) urethane (POSS-PCU) using plasma polymerization process to induce osteogenic differentiation of adipose derived stem cells (ADSCs). Optimization of plasma polymerization process was carried out keeping cell culture application in mind. Thus, samples were rigorously tested for retention of amino groups under both dry and wet conditions. Physio-chemical characterization was carried out using ninhydrin test, X-ray photon spectroscopy, scanning electron microscopy, and static water contact analysis. Results from physio chemical characterization shows that functionalization of the amino group is not stable under wet conditions and optimization of plasma process is required for stable bonding of amino groups to the POSS-PCU polymer. Optimized samples were later tested in vitro in short and long-term culture to study differentiation of ADSCs on amino modified samples. Short-term cell culture shows that initial cell attachment was significantly (p < 0.001) improved on amine modified samples (NH2-POSS-PCU) compared to unmodified POSS-PCU. NH2-POSS-PCU samples also facilitates osteogenic differentiation of ADSCs as confirmed by immunological staining of cells for extracellular markers such as collagen Type I and osteopontin. Quantification of total collagen and ALP activity also shows significant (p < 0.001) increase on NH2-POSS-PCU samples compared to unmodified POSS-PCU. A pilot study also confirms that these optimized amino modified POSS-PCU samples can further be functionalized using bone inducing peptide such as KRSR using conventional wet chemistry. This further provides an opportunity for biofunctionalization of the polymer for various tissue specific applications.
authors.affiliation: UCL Centre for Nanotechnology and Regenerative Medicine, Division of Surgery & Interventional Science, University College London , London, United Kingdom , NW3 2PF.|Université Paris Sud , Orthopedic Surgery, Hôpital Saint Antoine, 184, Rue du Faubourg-Saint-Antoine, Paris, France , 75012.
authors.forename: Camilo
authors.initials: C
authors.lastname: Chaves
authors.affiliation: UCL Centre for Nanotechnology and Regenerative Medicine, Division of Surgery & Interventional Science, University College London , London, United Kingdom , NW3 2PF.
authors.forename: Feras
authors.initials: F
authors.lastname: Alshomer
authors.affiliation: Department of Chemistry, University College London , 20 Gordon Street, London, United Kingdom , WC1H 0AJ.
authors.forename: Robert G
authors.initials: RG
authors.lastname: Palgrave
authors.affiliation: UCL Centre for Nanotechnology and Regenerative Medicine, Division of Surgery & Interventional Science, University College London , London, United Kingdom , NW3 2PF.
authors.forename: Deepak M
authors.initials: DM
authors.lastname: Kalaskar
mesh_terms: D000499:Allylamine; D002254:Carbonates; D053761:Nanocomposites; D017646:Organosilicon Compounds; D010012:Osteogenesis; D010865:Pilot Projects; D011076:Polycarboxylate Cement; D013234:Stem Cells; D014508:Urea
chemical_list: D002254:Carbonates; D017646:Organosilicon Compounds; D011076:Polycarboxylate Cement; C005506:polycarbonate; D000499:Allylamine; D014508:Urea
keywords: adipose-derived stem cells; allylamine; osteogenic differentiation; plasma polymerization; polyhedral oligomeric silsequioxane poly(carbonate-urea) urethane