title: A humic substance analogue AQDS stimulates Geobacter sp. abundance and enhances pentachlorophenol transformation in a paddy soil.
abstract: Soil humic substances can be used as redox mediators in accelerating the biotransformation of organic pollutants, and humus-respiring bacteria are widely distributed in soils. However, the impact of humic substances on the soil microbial community during the biotransformation of organic pollutants is expected to be crucial while remains to be unclear. In this study, the biostimulation of indigenous microbial communities and the consequent effects on anaerobic transformation of pentachlorophenol (PCP) by a model humic substance, anthraquinone-2,6-disulfonate (AQDS), were systematically investigated in a paddy soil. The addition of AQDS was observed to increase the production of HCl-extractable Fe(II) and enhance the PCP transformation rates consequently. The pseudo-first-order rate constants of the PCP transformation showed a positive exponential relationship with the AQDS dosage. The terminal restriction fragment length polymorphism (T-RFLP) results indicated the substantial effect of added AQDS on soil microbial community. The enhanced abundance of Geobacter sp. was disclosed to be most critical for accelerated PCP transformation when with AQDS, in which Geobacter sp. functioned for promoting the generation of active Fe(II) and consequently enhancing the PCP transformation rates. The transformation rates of PCP were exponentially correlated with the abundance of Geobacter sp. positively. The findings are expected to improve the understanding of diversity and ubiquity of microorganisms in humic substances-rich soils for accelerating the transformations of soil chlorinated pollutants.
authors.affiliation: Guangdong Key Laboratory of Agricultural Environment Pollution Integrated Control, Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, China.
authors.forename: Manjia
authors.initials: M
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550009, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Tong
authors.affiliation: State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550009, China. Electronic address: liuchengshuai@vip.gyig.ac.cn.
authors.forename: Chengshuai
authors.initials: C
authors.lastname: Liu
authors.affiliation: Guangdong Key Laboratory of Agricultural Environment Pollution Integrated Control, Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Chen
authors.affiliation: Guangdong Key Laboratory of Agricultural Environment Pollution Integrated Control, Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, China. Electronic address: cefbli@soil.gd.cn.
authors.forename: Fangbai
authors.initials: F
authors.lastname: Li
authors.affiliation: Guangdong Key Laboratory of Agricultural Environment Pollution Integrated Control, Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, China.
authors.forename: Jiangtao
authors.initials: J
authors.lastname: Qiao
mesh_terms: D000880:Anthraquinones / Q000737:chemistry*; D002681:China; D002713:Chlorine / Q000737:chemistry; D018628:Gene Dosage; D045302:Geobacter / Q000378:metabolism*; D054879:Halogenation; D006812:Humic Substances; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000737:chemistry; D010084:Oxidation-Reduction; D010416:Pentachlorophenol / Q000378:metabolism*; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000737:chemistry; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000880:Anthraquinones; D006812:Humic Substances; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; C408124:anthraquinone-2,6-disulfonate; D002713:Chlorine; D010416:Pentachlorophenol; D007501:Iron
keywords: Electron mediator; PCP; Reductive dechlorination; Soil detoxification; Soil microbial remediation