title: Quantifying Instrumental Artifacts in Folding Kinetics Measured by Single-Molecule Force Spectroscopy.
abstract: Force spectroscopy is commonly used to measure the kinetics of processes occurring in single biological molecules. These measurements involve attaching the molecule of interest to micron-sized or larger force probes via compliant linkers. Recent theoretical work has described how the properties of the probes and linkers can alter the observed kinetics from the intrinsic behavior of the molecule in isolation. We applied this theory to estimate the errors in measurements of folding made using optical tweezers. Errors in the folding rates arising from instrument artifacts were only ∼20% for constant-force measurements of DNA hairpins with typical choices of linker length and probe size. Measurements of transition paths using a constant trap position at high trap stiffness were also found to be in the low-artifact limit. These results indicate that typical optical trap measurements of kinetics reflect the dynamics of the molecule fairly well, and suggest practical limitations on experimental design to ensure reliable kinetic measurements.
authors.affiliation: Department of Physics, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Krishna
authors.initials: K
authors.lastname: Neupane
authors.affiliation: Department of Physics, University of Alberta, Edmonton, Alberta, Canada; National Institute for Nanotechnology, National Research Council, Edmonton, Alberta, Canada. Electronic address: michael.woodside@ualberta.ca.
authors.forename: Michael T
authors.initials: MT
authors.lastname: Woodside
mesh_terms: D016477:Artifacts; D004247:DNA / Q000737:chemistry*; D007700:Kinetics; D009690:Nucleic Acid Conformation; D052898:Optical Tweezers; D013057:Spectrum Analysis / Q000295:instrumentation*
chemical_list: D004247:DNA