title: Characterization of protein expression levels with label-free detected reverse phase protein arrays.
abstract: In reverse-phase protein arrays (RPPA), one immobilizes complex samples (e.g., cellular lysate, tissue lysate or serum etc.) on solid supports and performs parallel reactions of antibodies with immobilized protein targets from the complex samples. In this work, we describe a label-free detection of RPPA that enables quantification of RPPA data and thus facilitates comparison of studies performed on different samples and on different solid supports. We applied this detection platform to characterization of phosphoserine aminotransferase (PSAT) expression levels in Acanthamoeba lysates treated with artemether and the results were confirmed by Western blot studies.
authors.affiliation: Department of Optical Science and Engineering, Shanghai Engineering Research Center for Ultra-Precision Optical Manufacturing, Green Photoelectron Platform, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Fudan University, Shanghai, 200433, China.
authors.forename: Xuexue
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.
authors.forename: Yihong
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Department of Optical Science and Engineering, Shanghai Engineering Research Center for Ultra-Precision Optical Manufacturing, Green Photoelectron Platform, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Fudan University, Shanghai, 200433, China.
authors.forename: Chenggang
authors.initials: C
authors.lastname: Zhu
authors.affiliation: Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.
authors.forename: Junlong
authors.initials: J
authors.lastname: Cai
authors.affiliation: Department of Physics, University of California, Davis, CA, 95616, USA.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Department of Physics, University of California, Davis, CA, 95616, USA.
authors.forename: James P
authors.initials: JP
authors.lastname: Landry
authors.affiliation: Institutes of Biomedical Science, Fudan University, Shanghai, 200032, China.
authors.forename: Fengyun
authors.initials: F
authors.lastname: Zheng
authors.affiliation: Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.
authors.forename: Xunjia
authors.initials: X
authors.lastname: Cheng
authors.affiliation: Department of Optical Science and Engineering, Shanghai Engineering Research Center for Ultra-Precision Optical Manufacturing, Green Photoelectron Platform, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Fudan University, Shanghai, 200433, China. Electronic address: fyy@fudan.edu.cn.
authors.forename: Yiyan
authors.initials: Y
authors.lastname: Fei
mesh_terms: D048248:Acanthamoeba castellanii / Q000737:chemistry / Q000378:metabolism*; D040081:Protein Array Analysis / Q000379:methods*; D015800:Protozoan Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D015800:Protozoan Proteins
keywords: Label-free detection; Normalization; Quantification; Reverse phase protein arrays