title: New Sulfated Oligosaccharides Produced by Pseudomonas β-Agarase from Gracilaria verrucosa Polysaccharide.
abstract: Polysaccharide (partially sulfated agarose) with macrophage-stimulation activity, derived from Gracilaria verrucosa, was decomposed by two types of β-agarase (agarases II and IV) from Pseudomonas sp. O-148. The hydrolysates were fractionated with ethanol precipitation and anion-exchange chromatography. The resulting anionic oligosaccharides with sulfate groups were investigated by (13)C-NMR spectroscopy. While the spectra of oligosaccharides produced by agarase IV showed identical patterns with those by β-agarase I from Pseudomonas atlantica and indicated the location of a sulfated saccharide unit on the non-reducing end, another new type of saccharide was found in the products by agarase II. The novel oligosaccharides by agarase II had a neoagarobiose unit on their non-reducing end and had sulfated units internally. This indicated the novelty of agarase II in cleavage fashion.
authors.affiliation: a Shin Nihon Chemical Co., Ltd.
authors.forename: K
authors.initials: K
authors.lastname: Nomura
authors.affiliation: a Shin Nihon Chemical Co., Ltd.
authors.forename: Y
authors.initials: Y
authors.lastname: Naitoh
authors.affiliation: a Shin Nihon Chemical Co., Ltd.
authors.forename: S
authors.initials: S
authors.lastname: Muramatsu
authors.affiliation: b Research and Development Center, Showa Sangyo Co., Ltd.
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshizawa
authors.affiliation: b Research and Development Center, Showa Sangyo Co., Ltd.
authors.forename: J
authors.initials: J
authors.lastname: Tsunehiro
authors.affiliation: b Research and Development Center, Showa Sangyo Co., Ltd.
authors.forename: F
authors.initials: F
authors.lastname: Fukui
authors.affiliation: a Shin Nihon Chemical Co., Ltd.
authors.forename: M
authors.initials: M
authors.lastname: Itoh
keywords: preneoagarobiose unit; sulfated oligosaccharide; β-agarase