title: Lentiviral Transduction of Neurons in Adult Brain: Evaluation of Inflammatory Response and Cognitive Effects in Mice.
abstract: We evaluated the effect of hippocampal injection of lentiviral particles p156-CMV-EGFP on behavior, learning, and microglial Iba1(+) cells activation in mice. Testing in the open field and elevated plus-maze revealed higher anxiety levels in lentiviral-injected mice in comparison with animals injected with vehicle. At the same time, lentivirus injection did not change learning and memory of mice in the hippocampal-dependent fear conditioning task. Microglia density in lentivirus-injected mice was significantly higher than in vehicle-injected mice. Thus, hippocampal injection of lentiviral particles with minimum content of transgenes produced evident inflammation process, changed anxiety level of experimental animals, but had no effect on hippocampal-dependent learning and memory.
authors.affiliation: National Research Center Kurchatov Institute, Moscow, Russia.
authors.forename: T A
authors.initials: TA
authors.lastname: Kunitsyna
authors.affiliation: National Research Center Kurchatov Institute, Moscow, Russia. oivashkina@gmail.com.
authors.forename: O I
authors.initials: OI
authors.lastname: Ivashkina
authors.affiliation: National Research Center Kurchatov Institute, Moscow, Russia.
authors.forename: M A
authors.initials: MA
authors.lastname: Roshchina
authors.affiliation: National Research Center Kurchatov Institute, Moscow, Russia.
authors.forename: K A
authors.initials: KA
authors.lastname: Toropova
authors.affiliation: National Research Center Kurchatov Institute, Moscow, Russia.
authors.forename: K V
authors.initials: KV
authors.lastname: Anokhin
mesh_terms: D000818:Animals; D056547:CA1 Region, Hippocampal / Q000276:immunology / Q000821:virology*; D003071:Cognition; D018891:Dentate Gyrus / Q000276:immunology / Q000821:virology*; D016086:Lentivirus / Q000235:genetics / Q000276:immunology*; D008297:Male; D018782:Maze Learning; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000276:immunology / Q000821:virology*; D014161:Transduction, Genetic
keywords: behavior; brain; lentivirus; microglia; transduction