title: Novel microsatellite markers for Distylium lepidotum (Hamamelidaceae) endemic to the Ogasawara Islands.
abstract: BACKGROUND
  Distylium lepidotum is a small tree endemic to the Ogasawara Islands located in the northwestern Pacific Ocean. This species is a sole food for an endemic locust, Boninoxya anijimensis. Here, we developed microsatellite markers to investigate genetic diversity and genetic structure and to avoid a genetic disturbance after transplantation to restore the Ogasawara Islands ecosystem.
  
  
  RESULTS
  Microsatellite markers with perfect dinucleotide repeats were developed using the next-generation sequencing Illumina MiSeq Desktop Sequencer. Thirty-two primer pairs were characterized in two D. lepidotum populations on Chichijima and Hahajima Islands of the Ogasawara Islands. The number of alleles for the markers ranged from three to 23 per locus in the two populations. Expected heterozygosity per locus in each population ranged from 0.156 to 0.940 and 0.368 to 0.845, respectively.
  
  
  CONCLUSIONS
  These microsatellite markers will be useful for future population genetics studies of D. lepidotum and provide a basis for conservation management of the Ogasawara Islands.
authors.affiliation: Laboratory of Wildlife Ecology, Forestry and Forest Products Research Institute, 1 Matsunosato, Tsukuba, Ibaraki, 305-8687, Japan. sugaikyoko15@gmail.com.
authors.forename: Kyoko
authors.identifier: http://orcid.org/0000-0003-2426-6156
authors.initials: K
authors.lastname: Sugai
authors.affiliation: Laboratory of Ecological Genetics, Forestry and Forest Products Research Institute, 1 Matsunosato, Tsukuba, Ibaraki, 305-8687, Japan.
authors.forename: Suzuki
authors.initials: S
authors.lastname: Setsuko
mesh_terms: D000483:Alleles; D003247:Conservation of Natural Resources; D017753:Ecosystem; D056426:Genetic Loci; D014644:Genetic Variation; D005828:Genetics, Population; D029201:Hamamelidaceae / Q000145:classification / Q000235:genetics*; D006579:Heterozygote; D059014:High-Throughput Nucleotide Sequencing; D062312:Islands; D007564:Japan; D018895:Microsatellite Repeats; D010140:Pacific Ocean; D010802:Phylogeny; D060047:Reproductive Isolation; D014197:Trees / Q000235:genetics
keywords: Distylium lepidotum; Next-generation sequencing; Ogasawara Islands; Population genetics; Simple sequence repeat