title: Fluorescent Determination of Double-stranded DNA with an Anionic 1,1'-Azonaphthalene Derivative.
abstract: Palatine chrome black 6BN (PCB6BN) is virtually non-fluorescent in an aqueous solution or in the presence of single-stranded DNA (ssDNA), whereas the fluorescence intensity of PCB6BN was linearly enhanced up to 300 μM of double-stranded DNA (dsDNA) base pairs. PCB6BN could be a useful fluorescent probe for quantifying dsDNA even when ssDNA is present for both heterogeneous and homogeneous assays.
authors.affiliation: a Research & Development Center, Unitika Ltd.
authors.forename: Y
authors.initials: Y
authors.lastname: Fukushima
authors.affiliation: a Research & Development Center, Unitika Ltd.
authors.forename: M
authors.initials: M
authors.lastname: Hayashi
authors.affiliation: a Research & Development Center, Unitika Ltd.
authors.forename: H
authors.initials: H
authors.lastname: Nakajima
keywords: anionic dye; dsDNA determination; fluorescent probe