title: In vitro digestibility and proteases inhibitory effect of several feedstuffs for Parachromis dovii juveniles and P. dovii hybrid larvae.
abstract: Parachromis dovii, a native cichlid from Costa Rica, is highly appreciated for its size and flesh quality. Further, P. dovii easily accept inert feed from the beginning of exogenous feeding; however, its growth is low compared to live food. For this reason, evaluation of several feedstuffs using two in vitro techniques was done. The quantification of the in vitro inhibitory effect of seven plant ingredients on the alkaline protease activity was done using enzymatic extracts from larvae samples of 6, 15, 22 and 30 days after hatching (DAH). The in vitro alkaline digestibility assays were run for six protein sources (from animal and plant origin) using the enzymatic extract from larvae 30 DAH. Independent of fish age, all plant feedstuffs reduced alkaline digestive proteases activity; however, the wheat flour (14.1 % at 6 DAH, 33.4 % at 15 DAH) and broken rice meal (51.6 % at 22 DAH) showed the lowest inhibition percentage of alkaline digestive activity, whereas the highest inhibition percentage was found with soybean and palm kernel meals (92.5 % at 30 DAH and 87.4 %, respectively) (P < 0.05). The alkaline proteases inhibition percentage of feedstuffs varied during larvae ontogeny. From six protein dietary sources tested, tankage and fish meal presented the highest in vitro digestibility values, 113.9 and 74.9 %, respectively. Contrary, the lowest digestibility was found for blood and soybean meals (38.07 and 19.82 %, respectively).
authors.affiliation: Laboratorio de Acuicultura Continental, Escuela de Ciencias Biológicas, Universidad Nacional, Heredia, 86-3000, Costa Rica.
authors.forename: Silvia
authors.initials: S
authors.lastname: Valverde-Chavarría
authors.affiliation: Laboratorio de Acuacultura, DACBIOL, Universidad Juárez Autónoma de Tabasco, Carretera Villahermosa Cárdenas Km 0.5, 86139, Villahermosa, Tabasco, Mexico.
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Álvarez-González
authors.affiliation: Laboratorio de Acuicultura Continental, Escuela de Ciencias Biológicas, Universidad Nacional, Heredia, 86-3000, Costa Rica.
authors.forename: Miguel
authors.initials: M
authors.lastname: Brais-Medina
authors.affiliation: Laboratorio de Acuicultura Continental, Escuela de Ciencias Biológicas, Universidad Nacional, Heredia, 86-3000, Costa Rica.
authors.forename: Elman
authors.initials: E
authors.lastname: Calvo-Elizondo
authors.affiliation: Laboratorio de Acuicultura Continental, Escuela de Ciencias Biológicas, Universidad Nacional, Heredia, 86-3000, Costa Rica. juan.ulloa.rojas@una.cr.
authors.forename: Juan B
authors.identifier: http://orcid.org/0000-0003-4464-1136
authors.initials: JB
authors.lastname: Ulloa-Rojas
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D023681:Cichlids / Q000254:growth & development / Q000378:metabolism*; D004044:Dietary Proteins / Q000494:pharmacology*; D010450:Endopeptidases / Q000378:metabolism*; D005260:Female; D005396:Fish Products; D029941:Fish Proteins / Q000378:metabolism*; D006868:Hydrolysis; D007814:Larva / Q000254:growth & development / Q000378:metabolism; D019684:Magnoliopsida; D008297:Male; D059392:Petroleum Pollution; D010940:Plant Proteins / Q000494:pharmacology; D011202:Poultry Products
chemical_list: D001426:Bacterial Proteins; D004044:Dietary Proteins; D029941:Fish Proteins; D010940:Plant Proteins; D010450:Endopeptidases; C087822:alkaline protease
keywords: Digestive proteases inhibitor; Fish larvae; In vitro digestibility; Parachromis dovii