title: Mucosal delivery of a combination adjuvant comprising emulsified fine particles and LD-indolicidin enhances serological immunity to inactivated influenza virus.
abstract: Here we investigate the immunogenicity of a combination adjuvant comprising emulsified fine particles (namely PELC) together with LD-indolicidin, a peptiomimetic stereoisomer of the bovine neutrophil peptide indolicidin. We demonstrated that intranasal vaccination with either PELC/LD-indolicidin or Alum enhances protective influenza-specific serological immunity in mice; however, the adjuvant potency of Alum was rather reduced when the mice vaccinated orally with formulated influenza vaccines. The information gathered from this study will enhance our effort in the formulation design as well as the optimization of alternative administration routes for prophylactic vaccines against emerging infectious diseases, in particular pandemic influenza.
authors.affiliation: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, 35053 Miaoli, Taiwan; Graduate Institute of Immunology, China Medical University, 40402 Taichung, Taiwan. Electronic address: huangminghsi@nhri.org.tw.
authors.forename: Ming-Hsi
authors.initials: MH
authors.lastname: Huang
authors.affiliation: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, 35053 Miaoli, Taiwan.
authors.forename: Shih-Hsiung
authors.initials: SH
authors.lastname: Dai
authors.affiliation: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Zhunan, 35053 Miaoli, Taiwan; Graduate Institute of Immunology, China Medical University, 40402 Taichung, Taiwan.
authors.forename: Pele
authors.initials: P
authors.lastname: Chong
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology*; D000281:Administration, Intranasal; D000284:Administration, Oral; D000534:Alum Compounds / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D023181:Antimicrobial Cationic Peptides / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000276:immunology*; D008807:Mice, Inbred BALB C; D009975:Orthomyxoviridae / Q000276:immunology*; D011091:Polyesters / Q000008:administration & dosage / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000494:pharmacology*; D013185:Squalene / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D000276:Adjuvants, Immunologic; D000534:Alum Compounds; D000914:Antibodies, Viral; D023181:Antimicrobial Cationic Peptides; D007252:Influenza Vaccines; D011091:Polyesters; C000613452:poly(ethylene glycol)-b-poly(lactide-co-epsilon-caprolactone); C073143:indolicidin; C041524:aluminum sulfate; D011092:Polyethylene Glycols; D013185:Squalene
keywords: Combination adjuvant; Host defense peptides; Mucosal vaccine; Vaccine delivery systems