title: Dual therapeutic functions of F-5 fragment in burn wounds: preventing wound progression and promoting wound healing in pigs.
abstract: Burn injuries are a leading cause of morbidity including prolonged hospitalization, disfigurement, and disability. Currently there is no Food and Drug Administration-approved burn therapeutics. A clinical distinction of burn injuries from other acute wounds is the event of the so-called secondary burn wound progression within the first week of the injury, in which a burn expands horizontally and vertically from its initial boundary to a larger area. Therefore, an effective therapeutics for burns should show dual abilities to prevent the burn wound progression and thereafter promote burn wound healing. Herein we report that topically applied F-5 fragment of heat shock protein-90α is a dual functional agent to promote burn wound healing in pigs. First, F-5 prevents burn wound progression by protecting the surrounding cells from undergoing heat-induced caspase 3 activation and apoptosis with increased Akt activation. Accordingly, F-5-treated burn and excision wounds show a marked decline in inflammation. Thereafter, F-5 accelerates burn wound healing by stimulating the keratinocyte migration-led reepithelialization, leading to wound closure. This study addresses a topical agent that is capable of preventing burn wound progression and accelerating burn wound healing.
authors.affiliation: Department of Dermatology, USC-Norris Comprehensive Cancer Center, University of Southern California Keck Medical Center , Los Angeles, California, USA.
authors.forename: Ayesha
authors.initials: A
authors.lastname: Bhatia
authors.affiliation: Department of Dermatology, USC-Norris Comprehensive Cancer Center, University of Southern California Keck Medical Center , Los Angeles, California, USA.
authors.forename: Kathryn
authors.initials: K
authors.lastname: O'Brien
authors.affiliation: Department of Dermatology, USC-Norris Comprehensive Cancer Center, University of Southern California Keck Medical Center , Los Angeles, California, USA.
authors.forename: Mei
authors.initials: M
authors.lastname: Chen
authors.affiliation: Department of Surgery, University of Southern California Keck Medical Center , Los Angeles, California, USA.
authors.forename: Alex
authors.initials: A
authors.lastname: Wong
authors.affiliation: Department of Surgery, University of Southern California Keck Medical Center , Los Angeles, California, USA.
authors.forename: Warren
authors.initials: W
authors.lastname: Garner
authors.affiliation: Department of Dermatology, USC-Norris Comprehensive Cancer Center, University of Southern California Keck Medical Center , Los Angeles, California, USA.
authors.forename: David T
authors.initials: DT
authors.lastname: Woodley
authors.affiliation: Department of Dermatology, USC-Norris Comprehensive Cancer Center, University of Southern California Keck Medical Center , Los Angeles, California, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Li