title: Expression of microRNAs of C19MC in Different Histological Types of Testicular Germ Cell Tumour.
abstract: BACKGROUND
  Testicular germ cell tumours (TGCTs) are the most common tumours in men aged from 20 to 40 years, with a steadily increasing incidence. This study aimed to characterize the expression of the miRNA cluster C19MC in TGCT and to evaluate the suitability of a C19MC miRNA as a serum biomarker.
  
  
  METHODS
  By quantitative reverse transcription PCR, we measured the expression of miR-517a-3p, miR-519a-3p, and miR-519c 3p in tissue samples of 25 TGCTs and the level of miR-517a-3p in serum samples obtained pre- and postoperatively from the same patients.
  
  
  RESULTS
  We detected a significantly higher expression of C19MC miRNAs in non-seminomas than in seminomas and in clinical stages 2 and 3 than in stage 1 in both tissue and serum samples.
  
  
  CONCLUSIONS
  miRNAs of C19MC are overexpressed in more aggressive types of TGCT, suggesting they contribute to malignancy. Furthermore, they might serve as serum biomarkers for these types of TGCT.
authors.affiliation: Centre for Human Genetics, University of Bremen, Bremen, Germany iflor@uni-bremen.de.
authors.forename: Inga
authors.initials: I
authors.lastname: Flor
authors.affiliation: Centre for Human Genetics, University of Bremen, Bremen, Germany.
authors.forename: Meike
authors.initials: M
authors.lastname: Spiekermann
authors.affiliation: Department of Pathology, Albertinen-Hospital, Hamburg, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Löning
authors.affiliation: Department of Urology, Albertinen-Hospital, Hamburg, Germany.
authors.forename: Klaus-Peter
authors.initials: KP
authors.lastname: Dieckmann
authors.affiliation: Centre for Human Genetics, University of Bremen, Bremen, Germany.
authors.forename: Gazanfer
authors.initials: G
authors.lastname: Belge
authors.affiliation: Centre for Human Genetics, University of Bremen, Bremen, Germany Institute of Medical Genetics, University Rostock Medical Centre, Rostock, Germany.
authors.forename: Jörn
authors.initials: J
authors.lastname: Bullerdiek
mesh_terms: D014408:Biomarkers, Tumor; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics*; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D009373:Neoplasms, Germ Cell and Embryonal / Q000235:genetics* / Q000473:pathology* / Q000601:surgery; D013736:Testicular Neoplasms / Q000235:genetics* / Q000473:pathology* / Q000601:surgery; D016896:Treatment Outcome
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs
keywords: C19MC; TGCT; biomarker; miRNA; serum