title: Purification of Levan Fructotransferase from Arthvobacter nicotinovoram GS-9 and Production of DFA IV from Levan by the Enzyme.
abstract: A bacterial strain, GS-9, isolated from soil as a levan-degrading microorganism produced an extracellular enzyme that converted levan into DFA IV. This strain was identified as Arthrobacter nicotinovorvans. The DFA IV-producing enzyme was specifically induced by levan. The enzyme was purified 60-fold from culture supernatant to give a single band on SDS-PAGE. The molecular weight of this enzyme was 52,000 by SDS-PAGE and a monomer by gel filtration. The enzyme gave DFA IV as a main product (> 75%), and fructose, levanbiose, and two unidentified oligosaccharides as minor products, and was identified as a novel levan fructotransferase.
authors.affiliation: a Laboratory of Applied Microbiology, Department of Bioscience and Chemistry, Faculty of Agriculture , Hokkaido University , Sapporo 060 , Japan.
authors.forename: K
authors.initials: K
authors.lastname: Saito
authors.affiliation: a Laboratory of Applied Microbiology, Department of Bioscience and Chemistry, Faculty of Agriculture , Hokkaido University , Sapporo 060 , Japan.
authors.forename: H
authors.initials: H
authors.lastname: Goto
authors.affiliation: a Laboratory of Applied Microbiology, Department of Bioscience and Chemistry, Faculty of Agriculture , Hokkaido University , Sapporo 060 , Japan.
authors.forename: A
authors.initials: A
authors.lastname: Yokota
authors.affiliation: a Laboratory of Applied Microbiology, Department of Bioscience and Chemistry, Faculty of Agriculture , Hokkaido University , Sapporo 060 , Japan.
authors.forename: F
authors.initials: F
authors.lastname: Tomita
keywords: Arthrobacter nicotinovorans GS-9; DFA IV; levan; levan fructotransferase