title: Raman microspectroscopy of Hematoporphyrins. Imaging of the noncancerous and the cancerous human breast tissues with photosensitizers.
abstract: Raman microspectroscopy combined with fluorescence were used to study the distribution of Hematoporphyrin (Hp) in noncancerous and cancerous breast tissues. The results demonstrate the ability of Raman spectroscopy to distinguish between noncancerous and cancerous human breast tissue and to identify differences in the distribution and photodegradation of Hematoporphyrin, which is a photosensitizer in photodynamic therapy (PDT), photodynamic diagnosis (PDD) and photoimmunotherapy (PIT) of cancer. Presented results show that Hematoporphyrin level in the noncancerous breast tissue is lower compared to the cancerous one. We have proved also that the Raman intensity of lipids and proteins doesn't change dramatically after laser light irradiation, which indicates that the PDT treatment destroys preferably cancer cells, in which the photosensitizer is accumulated. The specific subcellular localization of photosensitizer for breast tissues samples soaked with Hematoporphyrin was not observed.
authors.affiliation: Lodz University of Technology, Faculty of Chemistry, Institute of Applied Radiation Chemistry, Laboratory of Laser Molecular Spectroscopy, Wroblewskiego 15, 93-590 Lodz, Poland. Electronic address: brozek@mitr.p.lodz.pl.
authors.forename: B
authors.initials: B
authors.lastname: Brozek-Pluska
authors.affiliation: Lodz University of Technology, Faculty of Chemistry, Institute of Applied Radiation Chemistry, Laboratory of Laser Molecular Spectroscopy, Wroblewskiego 15, 93-590 Lodz, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Kopec
mesh_terms: D001940:Breast / Q000000981:diagnostic imaging*; D001943:Breast Neoplasms / Q000000981:diagnostic imaging*; D005260:Female; D006415:Hematoporphyrins / Q000032:analysis*; D006801:Humans; D061848:Optical Imaging / Q000379:methods*; D017319:Photosensitizing Agents / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D006415:Hematoporphyrins; D017319:Photosensitizing Agents
keywords: Fluorescence; Hematoporphyrin; Raman imaging