title: The high correlation between counts and area fractions of lipofuscin granules, a biomarker of oxidative stress in muscular dystrophies.
abstract: Images of cryostat unstained sections of two skeletal muscles, diaphragm and extensor digitorum longus (EDL), from wild-type normal and dystrophic mdx mice were captured with a fluorescence microscope, binarised and analysed by an automated procedure using ImageJ free software. The numbers, Feret diameters and areas of autofluorescent lipofuscin (LF)-like granules in the sections were determined from the binary images. The mean numbers of counted LF granules per mm3muscle tissue correlated highly (r ≥ 0.9) with the area fractions of the granules in sections of both normal and mdx muscles. The similar distribution patterns of granule sizes in sections of diaphragm and EDL muscles are consistent with the high correlations.
authors.affiliation: Tokushima University Graduate School, Tokushima, Japan. yoshiko.nakae@globe.ocn.ne.jp.|, 391 Kamigoryo Baba-cho, Kamikyo-ku, Kyoto, 602-0891, Japan. yoshiko.nakae@globe.ocn.ne.jp.
authors.forename: Yoshiko
authors.identifier: http://orcid.org/0000-0002-3672-0013
authors.initials: Y
authors.lastname: Nakae
authors.affiliation: University of Dundee, Dundee, UK.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Stoward
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D008062:Lipofuscin / Q000032:analysis*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D009136:Muscular Dystrophies / Q000378:metabolism* / Q000473:pathology; D061848:Optical Imaging; D018384:Oxidative Stress
chemical_list: D015415:Biomarkers; D008062:Lipofuscin
keywords: Image analysis; Lipofuscin; Mdx mouse; Muscular dystrophy; Quantification; Skeletal muscle