title: Immunogenicity of the recombinant HxuCBA proteins encoded by hxuCBA gene cluster of Haemophilus parasuis in mice.
abstract: Haemophilus parasuis causes serious economic losses in pigs, which is the etiology of Glässer's disease. In this study we studied the immunogenicity of proteins encoded by the hxuCBA gene cluster in H. parasuis. Through bioinformatics analysis, HxuC, HxuB, and HxuA proteins were found that they might have strong antigenicity, with 31 putative cytotoxic T-lymphocyte (CTL) epitopes and multiple B-cell antigenic determinants. Western blotting assay indicated that all these antigens are highly immunogenic. The antibody levels and the levels of IL-2, IL-4, IFN-γ in the groups of HxuA, HxuB, HxuC, HxuCBA (include HxuC, HxuB and HxuA proteins), and M-3 were observed to significantly increase with time post vaccination. HxuC, HxuB, HxuCBA and H. parasuis M-3 vaccinated groups showed a strong immune response and protection against challenge with 6.5×10(9)cfu (5×LD50) of H. parasuis M-3 strain in a mouse model, but HxuA group showed only a low level protection. Additionally, the immune response induced by all of the proteins reduced histopathological lesions and their antisera could inhibit the growth of H. parasuis. We concluded that HxuC, HxuB and HxuCBA may have potential for use as a recombinant subunit vaccine against H. parasuis challenge.
authors.affiliation: Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Wen
authors.affiliation: Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Yan
authors.affiliation: Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Wen
authors.affiliation: Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Sanjie
authors.initials: S
authors.lastname: Cao
authors.affiliation: Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Lvqin
authors.initials: L
authors.lastname: He
authors.affiliation: Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Lingqiang
authors.initials: L
authors.lastname: Ding
authors.affiliation: Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Luhua
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhou
authors.affiliation: Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Huang
authors.affiliation: Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wu
authors.affiliation: Research Center of Swine Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China. Electronic address: 393248125@qq.com.
authors.forename: Xintian
authors.initials: X
authors.lastname: Wen
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D001428:Bacterial Vaccines / Q000235:genetics / Q000276:immunology; D003001:Cloning, Molecular; D016207:Cytokines / Q000378:metabolism; D004926:Escherichia coli; D006192:Haemophilus Infections / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control; D044137:Haemophilus parasuis / Q000235:genetics / Q000276:immunology*; D008168:Lung / Q000473:pathology; D051379:Mice; D005810:Multigene Family; D011994:Recombinant Proteins; D013154:Spleen / Q000473:pathology; D064166:Vaccine Potency; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D016207:Cytokines; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic
keywords: Haemophilus parasuis; Outer membrane proteins; Subunit vaccine; hxuCBA gene cluster