title: Changes in the membrane fatty acid composition in Anoxybacillus flavithermus subsp. yunnanensis E13Tas response to solvent stress.
abstract: Anoxybacillus flavithermus subsp. yunnanensis is currently the first species of strictly thermophilic bacteria that is able to tolerate a broad range of solvents. Unlike most of solvent-tolerant mesophilic bacteria, the bacterium does not synthesize unsaturated fatty acids. Our results revealed that in growing cells of A. flavithermus subsp. yunnanensis E13T, ethanol and toluene resulted in an increase in straight-chain fatty acids, mainly C16:0, leading to a more rigid membrane. Moreover, the increase in straight-chain fatty acids caused by ethanol was much higher than that of toluene. High temperature had little effect on the fatty acid composition by itself, whereas the combined conditions of high temperature and ethanol caused the dramatic increase in straight-chain fatty acids (mainly C16:0), that was balanced by decreasing branched fatty acids. The increase was also temperature dependent. The proportion of C16:0 further increased above 60 °C. No similar evidence was found in four other species of Anoxybacillus. The results suggested that A. flavithermus subsp. yunnanesis seems to develop a different response to solvents compared to its mesophilic counterparts, which consist of an increase in the saturated straight/branched ratio.
authors.affiliation: Engineering Technology Research Center of Microorganisms and Biocatalysis, School of Life Sciences, Anhui University, Hefei, 230039, Anhui, People's Republic of China. pph0259@126.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Peng
authors.affiliation: Engineering Technology Research Center of Microorganisms and Biocatalysis, School of Life Sciences, Anhui University, Hefei, 230039, Anhui, People's Republic of China.
authors.forename: Lu
authors.initials: L
authors.lastname: Yi
authors.affiliation: Engineering Technology Research Center of Microorganisms and Biocatalysis, School of Life Sciences, Anhui University, Hefei, 230039, Anhui, People's Republic of China.
authors.forename: Xiaohan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Engineering Technology Research Center of Microorganisms and Biocatalysis, School of Life Sciences, Anhui University, Hefei, 230039, Anhui, People's Republic of China.
authors.forename: Yazhong
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Engineering Technology Research Center of Microorganisms and Biocatalysis, School of Life Sciences, Anhui University, Hefei, 230039, Anhui, People's Republic of China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Engineering Technology Research Center of Microorganisms and Biocatalysis, School of Life Sciences, Anhui University, Hefei, 230039, Anhui, People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: He
mesh_terms: D058677:Anoxybacillus / Q000378:metabolism*; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism; D005227:Fatty Acids / Q000737:chemistry* / Q000378:metabolism; D006358:Hot Temperature; D007218:Industrial Microbiology; D012997:Solvents / Q000737:chemistry / Q000378:metabolism; D013696:Temperature
chemical_list: D005227:Fatty Acids; D012997:Solvents; D000431:Ethanol
keywords: Anoxybacillus flavithermus ssp. yunnanesis; High temperature; Organic solvent-tolerant; Straight-chain fatty acid; Stress response