title: Evaluation of a Single Intra-Articular Injection of Autologous Protein Solution for Treatment of Osteoarthritis in a Canine Population.
abstract: OBJECTIVE
  To evaluate the safety and efficacy of an intra-articular injection of autologous protein solution (APS) for treatment of canine osteoarthritis (OA).
  
  
  METHODS
  Prospective, randomized, blinded, placebo-controlled pilot clinical trial.
  
  
  METHODS
  Client-owned dogs with single limb lameness because of OA in a stifle or elbow joint (n=21).
  
  
  METHODS
  Lame dogs, confirmed with OA by physical and lameness examination and imaging, were randomly assigned to control or treatment groups. Owners, blinded to treatment, scored pain (University of Pennsylvania Canine Brief Pain Inventory) and lameness severity (Hudson Visual Analogue Scale [HVAS]). Weight-bearing was assessed by kinetic gait analysis. Dogs were injected intra-articularly with APS (treatment group) or saline solution (control group). Evaluations were performed before injection, and 2 and 12 weeks post-injection.
  
  
  RESULTS
  Compared to pretreatment values, APS treatment data showed a significant improvement in week 12 pain scores (improved 25.6% over baseline), lameness scores (improved 15% over baseline) and peak vertical force (PVF; N/kg; increased 14.9% of baseline), as well as vertical impulse (Ns/kg) and PVF normalized to stance time (N/kg/s). Control group dogs improved at week 2 in owner assigned indices, but not force plate values and had no significant improvement in scores or force plate values from pretreatment values at 12 weeks.
  
  
  CONCLUSIONS
  APS injection reduced pain and lameness scores and increased weight-bearing associated with the OA-affected joint in dogs at 12 weeks providing preliminary evidence that APS therapy may be beneficial in the treatment of OA in dogs and supporting pursuit of additional studies.
authors.affiliation: Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio.
authors.forename: Audrey W
authors.initials: AW
authors.lastname: Wanstrath
authors.affiliation: Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio.
authors.forename: Bianca F
authors.initials: BF
authors.lastname: Hettlich
authors.affiliation: Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio.
authors.forename: Lillian
authors.initials: L
authors.lastname: Su
authors.affiliation: Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio.
authors.forename: Ashley
authors.initials: A
authors.lastname: Smith
authors.affiliation: Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio.
authors.forename: Lisa J
authors.initials: LJ
authors.lastname: Zekas
authors.affiliation: Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio.
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Allen
authors.affiliation: Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio.
authors.forename: Alicia L
authors.initials: AL
authors.lastname: Bertone
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000008:administration & dosage / Q000627:therapeutic use*; D004283:Dog Diseases / Q000628:therapy*; D004285:Dogs; D007270:Injections, Intra-Articular / Q000662:veterinary; D007794:Lameness, Animal / Q000628:therapy*; D010003:Osteoarthritis / Q000628:therapy / Q000662:veterinary*; D010146:Pain / Q000662:veterinary; D010147:Pain Measurement; D011446:Prospective Studies; D016474:Weight-Bearing
chemical_list: D001798:Blood Proteins