title: Merging and comparing three mitochondrial markers for phylogenetic studies of Eurasian reindeer (Rangifer tarandus).
abstract: Phylogenetic analyses provide information that can be useful in the conservation of genetic variation by identifying intraspecific genetic structure. Reconstruction of phylogenetic relationships requires the use of markers with the appropriate amount of variation relative to the timeframe and purpose of the study. Here, genetic structure and clustering are inferred from comparative analyses of three widely used mitochondrial markers, the CR, cytb and the COI region, merged and separately, using Eurasian reindeer as a model. A Bayesian phylogeny and a MJ network, both based on the merged dataset, indicate several distinct maternal haplotype clusters within Eurasian reindeer. In addition to confirm previously described clusters, two new subclusters were found. When comparing the results from the merged dataset with the results from analyses of the three markers separately, similar clustering was found in the CR and COI phylogenies, whereas the cytb region showed poor resolution. Phylogenetic analyses of the merged dataset and the CR revealed congruent results, implying that single sequencing analysis of the CR is an applicable method for studying the haplotype structure in Eurasian reindeer.
authors.affiliation: Department of Environmental Studies University College of Southeast Norway Bø in Telemark Norway; Department of Basic Sciences and Aquatic Medicine Norwegian University of Life Sciences Oslo Norway.
authors.forename: Kjersti S
authors.initials: KS
authors.lastname: Kvie
authors.affiliation: Department of Environmental Studies University College of Southeast Norway Bø in Telemark Norway.
authors.forename: Jan
authors.initials: J
authors.lastname: Heggenes
authors.affiliation: Department of Basic Sciences and Aquatic Medicine Norwegian University of Life Sciences Oslo Norway.
authors.forename: Knut H
authors.initials: KH
authors.lastname: Røed
keywords: COI; CR; Rangifer tarandus; cytb; mitochondrial markers; phylogeny