title: Single objective light-sheet microscopy for high-speed whole-cell 3D super-resolution.
abstract: We have developed a method for performing light-sheet microscopy with a single high numerical aperture lens by integrating reflective side walls into a microfluidic chip. These 45° side walls generate light-sheet illumination by reflecting a vertical light-sheet into the focal plane of the objective. Light-sheet illumination of cells loaded in the channels increases image quality in diffraction limited imaging via reduction of out-of-focus background light. Single molecule super-resolution is also improved by the decreased background resulting in better localization precision and decreased photo-bleaching, leading to more accepted localizations overall and higher quality images. Moreover, 2D and 3D single molecule super-resolution data can be acquired faster by taking advantage of the increased illumination intensities as compared to wide field, in the focused light-sheet.
authors.affiliation: Department of Physics and Astronomy, University of New Mexico, 1919 Lomas Blvd NE, Albuquerque, NM 87131, USA; Department of Pathology, University of New Mexico, 2325 Camino de Salud, Albuquerque, NM 87131, USA.
authors.forename: Marjolein B M
authors.initials: MB
authors.lastname: Meddens
authors.affiliation: Department of Physics and Astronomy, University of New Mexico, 1919 Lomas Blvd NE, Albuquerque, NM 87131, USA; Current address: Weldon School of Biomedical Engineering, College of Engineering, Purdue University, West Lafayette, IN 47907, USA.
authors.forename: Sheng
authors.initials: S
authors.lastname: Liu
authors.affiliation: Sandia National Laboratories, 1515 Eubank SE, Albuquerque, NM 87123, USA.
authors.forename: Patrick S
authors.initials: PS
authors.lastname: Finnegan
authors.affiliation: Sandia National Laboratories, 1515 Eubank SE, Albuquerque, NM 87123, USA.
authors.forename: Thayne L
authors.initials: TL
authors.lastname: Edwards
authors.affiliation: Sandia National Laboratories, 1515 Eubank SE, Albuquerque, NM 87123, USA.
authors.forename: Conrad D
authors.initials: CD
authors.lastname: James
authors.affiliation: Department of Physics and Astronomy, University of New Mexico, 1919 Lomas Blvd NE, Albuquerque, NM 87131, USA.
authors.forename: Keith A
authors.initials: KA
authors.lastname: Lidke
keywords: (100.6640) Superresolution; (180.2520) Fluorescence microscopy; (180.6900) Three-dimensional microscopy; (230.3990) Micro-optical devices