title: Preparative separation of grape skin polyphenols by high-speed counter-current chromatography.
abstract: To develop an efficient method for large preparation of various individual polyphenols from white grape skins (Fernão Pires; Vitis vinifera) by preparative high-speed counter-current chromatography (HSCCC) and preparative-HPLC, an optimized preparative HSCCC condition with two-phase solvent system composed of Hex-EtOAc-H2O (1:50:50, v/v) was used to separate grape skin polyphenols into various fractions. Both the tail-head and head-tail elution modes were used with a flow rate of 3.0ml/min and a rotary speed of 950rpm. Afterwards, a preparative-HPLC separation was applied to isolate individual polyphenols in each of the fractions from HSCCC. Total of 7 fractions (Fraction A to G) were obtained from grape skin extract by HSCCC. After preparative-HPLC isolation, fifteen individual compounds were obtained, most of which presented high yields and purity (all over 90%). The HSCCC method followed with preparative-HPLC appeared to be convenient and economical, constituting an efficient strategy for the isolation of grape skin polyphenols.
authors.affiliation: School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, 110016 Shenyang, China.
authors.forename: Lanxin
authors.initials: L
authors.lastname: Luo
authors.affiliation: School of Functional Food and Wine, Shenyang Pharmaceutical University, 110016 Shenyang, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Cui
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, 110016 Shenyang, China; Department of Pharmacy, Jiamusi University, 154007 Jiamusi, China.
authors.forename: Shuting
authors.initials: S
authors.lastname: Zhang
authors.affiliation: School of Functional Food and Wine, Shenyang Pharmaceutical University, 110016 Shenyang, China.
authors.forename: Lingxi
authors.initials: L
authors.lastname: Li
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, 110016 Shenyang, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, 110016 Shenyang, China.
authors.forename: Peiyu
authors.initials: P
authors.lastname: Zhou
authors.affiliation: School of Functional Food and Wine, Shenyang Pharmaceutical University, 110016 Shenyang, China; Pólo Dois Portos, Instituto National de Investigação Agrária e Veterinária, I.P., Quinta da Almoinha, 2565-191 Dois Portos, Portugal. Electronic address: sunbaoshan@syphu.edu.cn.
authors.forename: Baoshan
authors.initials: B
authors.lastname: Sun
mesh_terms: D001681:Biological Assay; D002294:Carcinoma, Squamous Cell / Q000473:pathology; D045744:Cell Line, Tumor / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution; D006258:Head and Neck Neoplasms / Q000473:pathology; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D059808:Polyphenols / Q000032:analysis* / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D027843:Vitis / Q000737:chemistry*
chemical_list: D059808:Polyphenols; D012997:Solvents
keywords: (+)-Catechin (PubChem CID: 107957); (+)-Catechin-phloroglucinol derivative (PubChem CID: 14009031); (−)-Epicatechin (PubChem CID: 72276); Astilbin (PubChem CID: 119258); Grape skins; HPLC separation; High-speed counter-current chromatography; Phloroglucinol (PubChem CID: 359); Polyphenols; Procyanidin B1 (PubChem CID: 11250133); Procyanidin B1 3-gallate (PubChem CID: 72193635); Procyanidin B2 (PubChem CID: 122738); Procyanidin B3 (PubChem CID: 146798); Procyanidin B4 (PubChem CID: 147299); Procyanidin C1 (PubChem CID: 169853); trans-Coutaric acid (PubChem CID: 57517924)