title: Preliminary evaluation of a new kit for differentiation of Mycobacterium tuberculosis complex species using Speed-Oligo MTBC.
abstract: We present the first evaluation of a novel molecular assay, the Speed-Oligo Mycobacterium tuberculosis complex (SO-MTBC), which is based on PCR combined with a dipstick for the differentiation of M. tuberculosis complex (MTBC) members. The results of this assay were compared with findings obtained using the Genotype MTBC assay. In this study, 189 strains of MTBC isolates from 2011 to 2014 were evaluated to determine the MTBC species. Most (174, 92 %) of the strains were identified as M. tuberculosissensu stricto, 7 (3.7 %) as Mycobacteriumbovis, 5 (2.6 %) as M. bovis bacillus Calmette-Guérin, 2 (1.1 %) as Mycobacteriumafricanum and 1 (0.5 %) as Mycobacteriumcaprae; no strains belonged to Mycobacteriummicroti and Mycobacteriumcanettii subsp. The concordance κ coefficient obtained was 0.96 with the results of the Genotype MTBC assay. SO-MTBC may represent a fast and easy-to-use alternative for differentiating among MTBC subspecies in laboratories with standard equipment.
authors.affiliation: Department of Microbiology, University Hospital Virgen de las Nieves, Avenida de Fuerzas Armadas s/n, 18014 Granada, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Lara-Oya
authors.affiliation: Department of Microbiology, University Hospital Virgen de las Nieves, Avenida de Fuerzas Armadas s/n, 18014 Granada, Spain.
authors.forename: Javier Moisés
authors.initials: JM
authors.lastname: Rodríguez-Granger
authors.affiliation: Department of Microbiology, University Hospital Virgen de las Nieves, Avenida de Fuerzas Armadas s/n, 18014 Granada, Spain.
authors.forename: María Del Carmen
authors.initials: MDC
authors.lastname: Liébana-Martos
authors.affiliation: Vircell S.L. Research, Calle Avicena, 18016 Granada, Spain.
authors.forename: Pablo
authors.initials: P
authors.lastname: Mendoza-López
authors.affiliation: Department of Microbiology, University Hospital Virgen de las Nieves, Avenida de Fuerzas Armadas s/n, 18014 Granada, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Sampedro-Martínez
authors.affiliation: Department of Microbiology, University Hospital Virgen de las Nieves, Avenida de Fuerzas Armadas s/n, 18014 Granada, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: Cobo
authors.affiliation: Department of Microbiology, School of Medicine, University of Granada, Avenida de Madrid, 11, 18012 Granada, Spain.
authors.forename: José
authors.initials: J
authors.lastname: Gutierrez-Fernández
authors.affiliation: Department of Microbiology, Torrecardenas Hospital, Paraje Torrecárdenas s/n, 04009 Almeria, Spain.
authors.forename: Miguel José
authors.initials: MJ
authors.lastname: Martínez-Lirola
authors.affiliation: Department of Microbiology, University Hospital Virgen de las Nieves, Avenida de Fuerzas Armadas s/n, 18014 Granada, Spain.
authors.forename: José María
authors.initials: JM
authors.lastname: Navarro-Marí
mesh_terms: D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics*; D009693:Nucleic Acid Hybridization / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D011933:Reagent Kits, Diagnostic; D014376:Tuberculosis / Q000175:diagnosis* / Q000382:microbiology*
chemical_list: D011933:Reagent Kits, Diagnostic