title: [Expression of two transcript variants of long noncoding RNA C6orf176 in non-small cell lung cancer and its clinical significance].
abstract: OBJECTIVE
  To explore two transcript variants expression of long noncoding RNA C6orf176 in non-small cell lung cancer (NSCLC) and the clinical pathological significance.
  
  
  METHODS
  The expressions of transcript variant 1 (TV1) and transcript variant 2 (TV2) of long noncoding RNA C6orf176 in 57 NSCLC and adjacent cancerous tissues were examined by qPCR with β-actin as internal control.
  
  
  RESULTS
  Based on the results of qPCR, for C6orf176-TV1, 42 cases were down-regulated and 15 cases were up-regulated. The C6orf176-TV1 level was correlated to the grade of differentiation (P<0.05) but it was not correlated with gender, age, smoking history, tumor type and TNM stage. The expression of C6orf176-TV1 had a potential value in diagnosis of NSCLC by receiver operator characteristic (ROC) curve. Area under curve (AUC) of ROC curve was 0.708 (95% CI 0.615 to 0. 802). The sensitivity and specificity were 51% and 88%, respectively. For C6orf176-TV2, 39 cases were down-regulated and 18 cases were up-regulated. The C6orf176-TV2 level was correlated with the grade of differentiation (P<0.05) but it was not correlated with gender, age, tumor size, smoking history and TNM stage. C6orf176-TV2 level had value in diagnosis of NSCLC by ROC curve. AUC of ROC curve was 0.64 (95% CI 0.531 to 0.749). The sensitivity and specificity were 49% and 75%, respectively. Of the 57 specimens, 53 cases were simultaneous up or down-regulation of C6orf176-TV1 and C6orf176-TV2. The correlation coefficient was 0.99.
  
  
  CONCLUSIONS
  The expression of C6orf176-TV1 or C6orf176-TV2 is down-regulated in NSCLC and it is correlated with the grade of differentiation. It may act as a diagnosis indicator for NSCLC patients.
authors.affiliation: Pathology and Pathophysiology, Medical Science Research Center, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Pathology and Pathophysiology, Medical Science Research Center, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Zhenzi
authors.initials: Z
authors.lastname: Peng
authors.affiliation: Pathology and Pathophysiology, Medical Science Research Center, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Yeping
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Pathology and Pathophysiology, Medical Science Research Center, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Dan
authors.initials: D
authors.lastname: He
authors.affiliation: Pathology and Pathophysiology, Medical Science Research Center, Xiangya Hospital, Central South University, Changsha 410008; Key Laboratory of Cancer Proteomics, National Health and Family Planning Commission, Changsha 410008, China.
authors.forename: Chaojun
authors.initials: C
authors.lastname: Duan
authors.affiliation: Department of General Surgery, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Xinying
authors.initials: X
authors.lastname: Li
mesh_terms: D000199:Actins; D019540:Area Under Curve; D002289:Carcinoma, Non-Small-Cell Lung; D002454:Cell Differentiation; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms; D062085:RNA, Long Noncoding; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction; D012907:Smoking; D014158:Transcription, Genetic; D015854:Up-Regulation
chemical_list: D000199:Actins; D062085:RNA, Long Noncoding