title: Application of cavitation system to accelerate aqueous enzymatic extraction of seed oil from Cucurbita pepo L. and evaluation of hypoglycemic effect.
abstract: Cavitation-accelerated aqueous enzymatic extraction (CAEE) of seed oil from Cucurbita pepo was performed. An enzyme cocktail comprised of cellulose, pectinase and proteinase can work synergistically in releasing the oil. The CAEE extraction conditions were optimized by a Plackett-Burman design followed by a central composite methodology. A maximal extraction yield of 58.06% was achieved under optimal conditions of vacuum degree -0.07, enzyme amount 1.05% and extraction time 69min. As compared to soxhlet extraction (SE)-derived oil, CAEE-derived oil exhibited similar physical properties and better oxidation stability. In addition, chemical composition analyzing showed that the content of linoleic acid obtained by CAEE (47.67%) was higher than that of SE (44.51%). Moreover, the IC50 of oil obtained by CAEE and SE, as measured by α-amylase inhibition assay, were 40.68μg/mL and 45.46μg/mL. All results suggest that CAEE represents an excellent alternative protocol for production of oil from oil-bearing materials.
authors.affiliation: Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme, 150086 Harbin, PR China; Biotechnology Research Institute, Heilongjiang Academy of Agricultural Sciences, 150086 Harbin, PR China; Key Laboratory of Crop and Livestock Molecular Breeding of Heilongjiang, Heilongjiang Academy of Agricultural Sciences, 150086 Harbin, PR China.
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: Li
authors.affiliation: Biotechnology Research Institute, Heilongjiang Academy of Agricultural Sciences, 150086 Harbin, PR China; Key Laboratory of Crop and Livestock Molecular Breeding of Heilongjiang, Heilongjiang Academy of Agricultural Sciences, 150086 Harbin, PR China. Electronic address: lizhugang@163.com.
authors.forename: Zhu-Gang
authors.initials: ZG
authors.lastname: Li
authors.affiliation: Biotechnology Research Institute, Heilongjiang Academy of Agricultural Sciences, 150086 Harbin, PR China; Key Laboratory of Crop and Livestock Molecular Breeding of Heilongjiang, Heilongjiang Academy of Agricultural Sciences, 150086 Harbin, PR China.
authors.forename: Xun
authors.initials: X
authors.lastname: Wang
authors.affiliation: Biotechnology Research Institute, Heilongjiang Academy of Agricultural Sciences, 150086 Harbin, PR China; Key Laboratory of Crop and Livestock Molecular Breeding of Heilongjiang, Heilongjiang Academy of Agricultural Sciences, 150086 Harbin, PR China.
authors.forename: Jun-Yan
authors.initials: JY
authors.lastname: Han
authors.affiliation: Biotechnology Research Institute, Heilongjiang Academy of Agricultural Sciences, 150086 Harbin, PR China; Key Laboratory of Crop and Livestock Molecular Breeding of Heilongjiang, Heilongjiang Academy of Agricultural Sciences, 150086 Harbin, PR China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, 150040 Harbin, PR China.
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Fu
authors.affiliation: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, 150040 Harbin, PR China.
authors.forename: Chun-Jian
authors.initials: CJ
authors.lastname: Zhao
mesh_terms: D028464:Cucurbita; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007004:Hypoglycemic Agents / Q000032:analysis* / Q000494:pharmacology; D019787:Linoleic Acid / Q000032:analysis; D059625:Liquid-Liquid Extraction / Q000379:methods; D010084:Oxidation-Reduction; D010938:Plant Oils / Q000032:analysis* / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry*; D014867:Water / Q000032:analysis*; D000516:alpha-Amylases / Q000032:analysis / Q000037:antagonists & inhibitors
chemical_list: D007004:Hypoglycemic Agents; D010938:Plant Oils; D014867:Water; D019787:Linoleic Acid; D000516:alpha-Amylases
keywords: Anti-diabetic activity; Arachidic (PubChem CID: 10467); Cavitation-accelerated aqueous enzymatic extraction; Cucurbita pepo L. seed oil; Fatty acid compositions; Linoleic acid (PubChem CID: 5280450); Linolenic acid (PubChem CID: 5280934); Myristic acid (PubChem CID: 11005); Oleic acid (PubChem CID: 445639); Palmitic acid (PubChem CID: 985); Palmitoleic acid (PubChem CID: 445638); Physicochemical properties; Plackett-Burman design; Stearic acid (PubChem CID: 5281)