title: Analysis of anthocyanin biosynthesis genes expression profiles in contrasting cultivars of Japanese plum (Prunus salicina L.) during fruit development.
abstract: Flavonoids are responsible of different fruit sensorial properties. In Japanese plum (Prunus salicina L.) these compounds are variable in both type and quantity during the different stages of fruit growth and maturation. Here we present the first study which determines the expression profile of structural genes of the flavonoid pathway and accumulation profiles of total phenols, proanthocyanidins and anthocyanins during fruit development stages in contrasting cultivars in Japanese plum. The biosynthesis of these compounds is differentially regulated in different tissues and cultivars. Our result showed that all pigmented tissues increased the expression of the leucoanthocyanidin dioxygenase (LDOX) gene, while all tissues without anthocyanin accumulation presented a minimal expression of LDOX. In addition, the regulation of putative transcription factors PsMYB10 and PsMYB1 were correlated positively and negatively with the pigmented tissues respectively, suggesting a critical and coordinated mechanism involved in the change of the fruit color.
authors.affiliation: Facultad de Agronomía e Ingeniería Forestal, Pontificia Universidad Católica de Chile, Avda. Vicuña Mackenna 4860, Macul, Región Metropolitana, Chile. Electronic address: mpgonza2@uc.cl.
authors.forename: Máximo
authors.initials: M
authors.lastname: González
authors.affiliation: Centro Regional de Investigación La Platina, Instituto de Investigaciones Agropecuarias, Unidad de Recursos Genéticos, Avda. Santa Rosa 11610, La Pintana, Región Metropolitana, Chile.
authors.forename: Erika
authors.initials: E
authors.lastname: Salazar
authors.affiliation: Facultad de Ingeniería, Pontificia Universidad Católica de Chile, Avda. Vicuña Mackenna 4860, Macul, Región Metropolitana, Chile.
authors.forename: Soledad
authors.initials: S
authors.lastname: Cabrera
authors.affiliation: Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Avenida Avda. Bernardo O'Higgins 340, Santiago, Región Metropolitana, Chile.
authors.forename: Pilar
authors.initials: P
authors.lastname: Olea
authors.affiliation: Facultad de Agronomía e Ingeniería Forestal, Pontificia Universidad Católica de Chile, Avda. Vicuña Mackenna 4860, Macul, Región Metropolitana, Chile.
authors.forename: Basilio
authors.initials: B
authors.lastname: Carrasco
mesh_terms: D000872:Anthocyanins / Q000096:biosynthesis* / Q000235:genetics; D005638:Fruit / Q000235:genetics* / Q000254:growth & development; D018506:Gene Expression Regulation, Plant; D010105:Oxygenases; D010940:Plant Proteins / Q000096:biosynthesis; D000068242:Prunus domestica / Q000235:genetics* / Q000254:growth & development; D059467:Transcriptome
chemical_list: D000872:Anthocyanins; D010940:Plant Proteins; D010105:Oxygenases; C105763:leucoanthocyanidin dioxygenase
keywords: Flavonoid pathway; PsMYB1; PsMYB10; Pulp; R2R3MYB; Skin