title: Isolation andIn vivoTransfer of Antigen Presenting Cells.
abstract: Transfer of antigen presenting cellsin vivois a method used by immunologists to examine the potency of antigen presentation by a selected population of cells. This method is most commonly used to analyze presentation of protein antigens to MHC class I or II restricted T cells, but it can also be used for studies of nonconventional antigens such as CD1-presented lipids. In a recent study focusing on CD1d-restricted glycolipid antigen presentation to Natural Killer T cells, we compared antigen presenting properties of splenic B cells, CD8αPosdendritc cells (DCs) and CD8αNegDCs (Aroraet al., 2014). This protocol describes the detailed method used for isolation of these cell populations, and their transfer into recipient mice to analyze their antigen presenting properties.
authors.affiliation: Department of Microbiology and Immunology, Albert Einstein College of Medicine, NY, USA.
authors.forename: Pooja
authors.initials: P
authors.lastname: Arora
authors.affiliation: Department of Microbiology and Immunology, Albert Einstein College of Medicine, NY, USA.
authors.forename: Shalu Sharma
authors.initials: SS
authors.lastname: Kharkwal
authors.affiliation: Department of Medicine, Albert Einstein College of Medicine, NY, USA.
authors.forename: Steven A
authors.initials: SA
authors.lastname: Porcelli