title: Validation of Minim typing for fast and accurate discrimination of extended-spectrum, beta-lactamase-producing Klebsiella pneumoniae isolates in tertiary care hospital.
abstract: Minim typing is derived from the multi-locus sequence typing (MLST). It targets the same genes, but sequencing is replaced by high resolution melt analysis. Typing can be performed by analysing six loci (6MelT), four loci (4MelT) or using data from four loci plus sequencing the tonB gene (HybridMelT). The aim of this study was to evaluate Minim typing to discriminate extended-spectrum beta-lactamase producing Klebsiella pneumoniae (ESBL-KLPN) isolates at our hospital. In total, 380 isolates were analyzed. The obtained alleles were assigned according to both the 6MelT and 4MelT typing scheme. In 97 isolates, the tonB gene was sequenced to enable HybridMelT typing. We found that the presented method is suitable to quickly monitor isolates of ESBL-KLPN; results are obtained in less than 2 hours and at a lower cost than MLST. We identified a local ESBL-KLPN outbreak and a comparison of colonizing and invasive isolates revealed a long term colonization of patients with the same strain.
authors.affiliation: Department of Internal Medicine - Hematology and Oncology, Masaryk University, Brno, Czech Republic; Department of Internal Medicine - Hematology and Oncology, University Hospital Brno, Brno, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Brhelova
authors.affiliation: Department of Clinical Microbiology, University Hospital Brno, Brno, Czech Republic.
authors.forename: Iva
authors.initials: I
authors.lastname: Kocmanova
authors.affiliation: Department of Internal Medicine - Hematology and Oncology, Masaryk University, Brno, Czech Republic; Department of Internal Medicine - Hematology and Oncology, University Hospital Brno, Brno, Czech Republic; CEITEC - Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Racil
authors.affiliation: Department of Clinical Microbiology, University Hospital Brno, Brno, Czech Republic.
authors.forename: Marketa
authors.initials: M
authors.lastname: Hanslianova
authors.affiliation: Department of Internal Medicine - Hematology and Oncology, Masaryk University, Brno, Czech Republic.
authors.forename: Mariya
authors.initials: M
authors.lastname: Antonova
authors.affiliation: Department of Internal Medicine - Hematology and Oncology, Masaryk University, Brno, Czech Republic; Department of Internal Medicine - Hematology and Oncology, University Hospital Brno, Brno, Czech Republic; CEITEC - Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
authors.forename: Jiri
authors.initials: J
authors.lastname: Mayer
authors.affiliation: Department of Internal Medicine - Hematology and Oncology, Masaryk University, Brno, Czech Republic; Department of Internal Medicine - Hematology and Oncology, University Hospital Brno, Brno, Czech Republic. Electronic address: mlengerova@fnbrno.cz.
authors.forename: Martina
authors.initials: M
authors.lastname: Lengerova
mesh_terms: D002353:Carrier State / Q000382:microbiology; D006801:Humans; D007710:Klebsiella Infections / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000145:classification* / Q000201:enzymology* / Q000302:isolation & purification; D058889:Molecular Typing / Q000379:methods*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D062606:Tertiary Care Centers; D013997:Time Factors; D044366:Transition Temperature; D001618:beta-Lactamases / Q000378:metabolism*
chemical_list: D001618:beta-Lactamases
keywords: ESBL; High-resolution melt analysis; Klebsiella pneumoniae; Minim typing; Multi-locus sequence typing