title: Pseudotenacibaculum haliotis gen. nov., sp. nov., a new member within the Tenacibaculum-Polaribacter clade of the family Flavobacteriaceae, isolated from the intestine of adult abalones, Haliotis diversicolor and H. discushannai.
abstract: Two rod-shaped marine bacterial strains named FDZSB0410T and FDZWPB0420, were isolated from the intestine of two adult abalone species, Haliotis diversicolor and Haliotis discushannai, respectively. The strains were non-flagellated, motile by gliding, oxidase-positive but catalase-negative, and agar-degrading. The two strains had identical 16S rRNA gene sequences with the highest sequence similarity less than 96.0 % with species of the genera Tenacibaculumand Polaribacter within the family Flavobacteriaceae. Phylogenetic analysis indicated the strains represented an independent cluster neighbouring with the species of the genera Tenacibaculum and Polaribacter. Strain FDZSB0410T can grow under conditions at 15 to 37 °C with optimum of 30 °C, salinity range from 3 to 4 % (NaCl, w/v) with optimum of 3 %, and pH range from 7 to 8 with optimum of pH 7. The predominant fatty acids of strain FDZSB0410T consisted of iso-C15 : 0, iso-C15 :1 G, summed feature 3 (comprising C16 : 1ω7c/C16 : 1ω6c), iso-C13 : 0, iso-C17 : 0 3-OH, and iso-C15 : 0 3-OH. The ubiquinone system detected was MK-6. The flexirubin pigment was absent, but the carotenoid was present. The polar lipids were identified as aminolipid, glycolipid, phosphatidylglycerol, an unknown phospholipid and four unknown lipids. The DNA G+C content was 35.3 mol%. On the basis of the genotypic and phenotypic characterizations, strain FDZSB0410T and strain FDZWPB0420 represent a novel species of a new genus in the family Flavobacteriaceae, for which the name Pseudotenacibaculum haliotis gen. nov., sp. nov. is proposed. The type strain of the type species is FDZSB0410T (=KCTC 52127T=MCCC 1A01897T).
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo, PR China.|Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, State of Oceanic Administration, South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Xiamen, PR China.
authors.forename: Zhaobin
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, State of Oceanic Administration, South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Xiamen, PR China.
authors.forename: Liping
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo, PR China.
authors.forename: Demin
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, State of Oceanic Administration, South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Xiamen, PR China.|School of Marine Sciences, Ningbo University, Ningbo, PR China.
authors.forename: Zongze
authors.initials: Z
authors.lastname: Shao
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D049851:Gastropoda / Q000382:microbiology*; D006017:Glycolipids / Q000737:chemistry; D007422:Intestines / Q000382:microbiology; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology; D017422:Sequence Analysis, DNA; D060493:Tenacibaculum / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006017:Glycolipids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C042455:menaquinone 6