title: Quali-quantitative analysis of the phenolic fraction of the flowers of Corylus avellana, source of the Italian PGI product "Nocciola di Giffoni": Isolation of antioxidant diarylheptanoids.
abstract: There is only limited information available on the chemical composition of the non-edible parts of Corylus avellana, source of the Italian PGI product "Nocciola di Giffoni" (hazelnut). An initial LC-MS profile of the methanolic extract of the male flowers of C. avellana, cultivar 'Tonda di Giffoni' led to the isolation of 12 compounds, of which the structures were elucidated by NMR spectroscopy. These were identified as three previously undescribed diarylheptanoids, named giffonins Q-S, along with nine known compounds. Furthermore, the quantitative determination of the main compounds occurring in the methanolic extract of C. avellana flowers was carried out by an analytical approach based on LC-ESI(QqQ)MS, using the Multiple Reaction Monitoring (MRM) experiment. In order to explore the antioxidant ability of C. avellana flowers, the methanolic extract and the isolated compounds were evaluated for their inhibitory effects on human plasma lipid peroxidation induced by H2O2 and H2O2/Fe(2+), by measuring the concentration of TBARS.
authors.affiliation: Dipartimento di Farmacia, Università degli Studi di Salerno, Via Giovanni Paolo II n. 132, 84084 Fisciano, SA, Italy.
authors.forename: Milena
authors.initials: M
authors.lastname: Masullo
authors.affiliation: Dipartimento di Farmacia, Università degli Studi di Salerno, Via Giovanni Paolo II n. 132, 84084 Fisciano, SA, Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Mari
authors.affiliation: Dipartimento di Farmacia, Università degli Studi di Salerno, Via Giovanni Paolo II n. 132, 84084 Fisciano, SA, Italy; PhD Program in Drug Discovery and Development, University of Salerno, Via Giovanni Paolo II 132, I-84084 Fisciano, SA, Italy.
authors.forename: Antonietta
authors.initials: A
authors.lastname: Cerulli
authors.affiliation: Dipartimento di Farmacia, Università degli Studi di Salerno, Via Giovanni Paolo II n. 132, 84084 Fisciano, SA, Italy.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Bottone
authors.affiliation: Department of General Biochemistry, Institute of Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/3, 90-236 Lodz, Poland.
authors.forename: Bogdan
authors.initials: B
authors.lastname: Kontek
authors.affiliation: Department of General Biochemistry, Institute of Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/3, 90-236 Lodz, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Olas
authors.affiliation: Dipartimento di Farmacia, Università degli Studi di Salerno, Via Giovanni Paolo II n. 132, 84084 Fisciano, SA, Italy.
authors.forename: Cosimo
authors.initials: C
authors.lastname: Pizza
authors.affiliation: Dipartimento di Farmacia, Università degli Studi di Salerno, Via Giovanni Paolo II n. 132, 84084 Fisciano, SA, Italy. Electronic address: piacente@unisa.it.
authors.forename: Sonia
authors.initials: S
authors.lastname: Piacente
mesh_terms: D000975:Antioxidants / Q000737:chemistry; D031211:Corylus / Q000737:chemistry*; D036381:Diarylheptanoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D035264:Flowers / Q000737:chemistry*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007558:Italy; D015227:Lipid Peroxidation; D019906:Nuclear Magnetic Resonance, Biomolecular; D010636:Phenols / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D000975:Antioxidants; D036381:Diarylheptanoids; D010636:Phenols; D006861:Hydrogen Peroxide
keywords: Antioxidant activity; Betulaceae; Corylus avellana; Diarylheptanoids; Flowers; Hazelnut; LC-ESI(QqQ)MS; Multiple Reaction Monitoring