title: Industrial application of selected lactic acid bacteria isolated from local semolinas for typical sourdough bread production.
abstract: Four obligate heterofermentative lactic acid bacteria (LAB) strains (Weissella cibaria PON10030 and PON10032 and Leuconostoc citreum PON 10079 and PON10080) were tested as single strain starters, mono-species dual strain starters, and multiple strain starter for the preparation and propagation of sourdoughs for the production of a typical bread at industrial level. The kinetics of pH and TTA during the daily sourdough refreshments indicated a correct acidification process for all trials. The concentration of lactic and acetic acid increased consistently during fermentation. The resulting molar ratios between these two organic acids in the experimental trials were lower than those observed in the control trial. The microbiological investigation showed levels of approximately 10(9) CFU/mL in almost all sourdoughs and the comparison of the genetic polymorphisms of the dominating LAB with those of the pure cultures evidenced the persistence of the added strains over time. The resulting breads were evaluated for several quality parameters. The breads with the greatest height were obtained with the quadruple combination of leuconostocs and weissellas. The highest softness was registered for the breads obtained from fermentations performed by W. cibaria PON10032 alone and in combination. The different inocula influenced also the color, the void fraction, the cell density and the mean cell area of the breads. Different levels of acids, alcohols, aldehydes, esters, hydrocarbons, ketones, terpenes, furans and phenol were emitted by the breads. The sensory tests indicated the breads from the sourdoughs fermented with the seven LAB inocula as sweeter and less acidic than control breads and the breads from the trials with the highest complexity of LAB inoculums were those more appreciated by tasters. A multivariate approach found strong differences among the trials. In particular, control breads and the breads obtained with different starter LAB were quite distant and a more strict relation was found among the productions carried out by W. cibaria strains. This study proved the suitability of the selected strains of L. citreum and W. cibaria for industrial-scale level applications in sourdough bread production.
authors.affiliation: Dipartimento Scienze Agrarie e Forestali, Università degli Studi di Palermo, Viale delle Scienze 4, 90128 Palermo, Italy.
authors.forename: Onofrio
authors.initials: O
authors.lastname: Corona
authors.affiliation: Dipartimento Scienze Agrarie e Forestali, Università degli Studi di Palermo, Viale delle Scienze 4, 90128 Palermo, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Alfonzo
authors.affiliation: Dipartimento Scienze Agrarie e Forestali, Università degli Studi di Palermo, Viale delle Scienze 4, 90128 Palermo, Italy.
authors.forename: Giusi
authors.initials: G
authors.lastname: Ventimiglia
authors.affiliation: Dipartimento Scienze Agrarie e Forestali, Università degli Studi di Palermo, Viale delle Scienze 4, 90128 Palermo, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Nasca
authors.affiliation: Dipartimento Scienze Agrarie e Forestali, Università degli Studi di Palermo, Viale delle Scienze 4, 90128 Palermo, Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Francesca
authors.affiliation: Dipartimento Scienze Agrarie e Forestali, Università degli Studi di Palermo, Viale delle Scienze 4, 90128 Palermo, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Martorana
authors.affiliation: Dipartimento Scienze Agrarie e Forestali, Università degli Studi di Palermo, Viale delle Scienze 4, 90128 Palermo, Italy.
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Moschetti
authors.affiliation: Dipartimento Scienze Agrarie e Forestali, Università degli Studi di Palermo, Viale delle Scienze 4, 90128 Palermo, Italy. Electronic address: luca.settanni@unipa.it.
authors.forename: Luca
authors.initials: L
authors.lastname: Settanni
mesh_terms: D019342:Acetic Acid / Q000032:analysis; D001939:Bread / Q000382:microbiology*; D005285:Fermentation; D005433:Flour / Q000382:microbiology*; D005516:Food Microbiology; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology; D019344:Lactic Acid / Q000032:analysis; D007934:Leuconostoc / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D011110:Polymorphism, Genetic; D058836:Weissella / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D019344:Lactic Acid; D019342:Acetic Acid
keywords: Industrial application; Leuconostoc citreum; Sourdough; Starter culture; Typical bread; Weissella cibaria