title: Dietary Protective Effects Against Hepatocellular Carcinoma Development in Mdr2-/- Knockout Mice.
abstract: OBJECTIVE
  The Mdr2(-/-) mouse develops early chronic cholestatic hepatitis and hepatocellularcarcinoma (HCC) when adult. We tested the effects of a restricted-calorie diet on HCC development in Mdr2(-/-) mice.
  
  
  METHODS
  Mdr2(-/-) mice (n=40, divided into two groups of 20 mice each) were randomized to receive ad libitum diet or restricted-calorie diet. Two mice from each group were sacrificed at 3 and 6 months, and liver tissue samples were removed for analysis. The remaining mice were fed their respective diets until the age of 30 months, at which time they were euthanized and livers were collected for analysis.
  
  
  RESULTS
  The restricted-calorie diet had partial chemopreventive effect on the development of HCC in Mdr2(-/-) mice. Moreover, mice with ad libitum diet had a median survival of 361 days, while the restricted-calorie group had a median survival of 500 days (p=0.0001).
  
  
  CONCLUSIONS
  A restricted diet might reduce the chance of developing HCC in patients at risk and could increase the protective action of anti-inflammatory agents.
authors.affiliation: Animal Facility (SAFU), Regina Elena National Cancer Institute, Rome, Italy.
authors.forename: Maria Pia
authors.initials: MP
authors.lastname: Gentileschi
authors.affiliation: Clinical Pathology Laboratory, Regina Elena National Cancer Institute, Rome, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Lattanzio
authors.affiliation: Centro Veterinario Gianicolense, Rome, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Menicagli
authors.affiliation: Campus Biomedico University, Rome, Italy.
authors.forename: Bruno
authors.initials: B
authors.lastname: Vincenzi
authors.affiliation: Clinical Pathology Laboratory, Regina Elena National Cancer Institute, Rome, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Cigliana
authors.affiliation: Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, Second University of Naples, Caserta, Italy.
authors.forename: Alfonso
authors.initials: A
authors.lastname: Baldi
authors.affiliation: Department of Research, Advanced Diagnostics, and Technological Innovation, Translational Research Area, Regina Elena National Cancer Institute, Rome, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Blandino
authors.affiliation: Department of Oncology, Faculty of Health Science, McMaster University, Hamilton, Canada.
authors.forename: Paola
authors.initials: P
authors.lastname: Muti
authors.affiliation: Animal Facility (SAFU), Regina Elena National Cancer Institute, Rome, Italy.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Fanciulli
authors.affiliation: Animal Facility (SAFU), Regina Elena National Cancer Institute, Rome, Italy info@enricospugnini.net.
authors.forename: Enrico P
authors.initials: EP
authors.lastname: Spugnini
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B / Q000502:physiology*; D000328:Adult; D000818:Animals; D031204:Caloric Restriction; D006528:Carcinoma, Hepatocellular / Q000517:prevention & control*; D004032:Diet; D006801:Humans; D008114:Liver Neoplasms, Experimental / Q000517:prevention & control*; D051379:Mice; D018345:Mice, Knockout; D020011:Protective Agents / Q000008:administration & dosage*; D000097202:ATP-Binding Cassette Sub-Family B Member 4
chemical_list: D018435:ATP Binding Cassette Transporter, Subfamily B; D020011:Protective Agents; D000097202:ATP-Binding Cassette Sub-Family B Member 4
keywords: Hepatocellular carcinoma; Mdr2−/− mouse; diet; prevention; ultrasonography