title: 808 nm-excited upconversion nanoprobes with low heating effect for targeted magnetic resonance imaging and high-efficacy photodynamic therapy in HER2-overexpressed breast cancer.
abstract: To avoid the overheating effect of excitation light and improve the efficacy of photodynamic therapy (PDT) of upconversion nanoplatform, a novel nanoprobe based on 808 nm-excited upconversion nanocomposites (T-UCNPs@Ce6@mSiO2) with low heating effect and deep penetration has been successfully constructed for targeted upconversion luminescence, magnetic resonance imaging (MRI) and high-efficacy PDT in HER2-overexpressed breast cancer. In this nanocomposite, photosensitizers (Ce6) were covalently conjugated inside of mesoporous silica to enhance the PDT efficacy by shortening the distance of fluorescence resonance energy transfer and to decrease the cytotoxicity by preventing the undesired leakage of Ce6. Compared with UCNPs@mSiO2@Ce6, UCNPs@Ce6@mSiO2 greatly promoted the singlet oxygen generation and amplified the PDT efficacy under the excitation of 808 nm laser. Importantly, the designed nanoprobe can greatly improve the uptake of HER2-positive cells and tumors by modifying the site-specific peptide, and the in vivo experiments showed excellent MRI and PDT via intravenous injection by modeling MDA-MB-435 tumor-bearing nude mice. Our strategy may provide an effective solution for overcoming the heating effect and improving the PDT efficacy of upconversion nanoprobes, and has potential application in visualized theranostics of HER2-overexpressed breast cancer.
authors.affiliation: Key Laboratory of Magnetic Materials and Devices & Key Laboratory of Additive Manufacturing Materials of Zhejiang Province & Division of Functional Materials and Nanodevices, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, PR China; Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of Education, Chemical Biology Key Laboratory of Hebei Province, College of Chemistry & Environmental Science, Hebei University, Baoding, 071002, PR China; Department of Medical Imaging, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, 210002, PR China.
authors.forename: Leyong
authors.initials: L
authors.lastname: Zeng
authors.affiliation: Key Laboratory of Magnetic Materials and Devices & Key Laboratory of Additive Manufacturing Materials of Zhejiang Province & Division of Functional Materials and Nanodevices, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, PR China; Laboratory for Microstructures, Institute of Materials, Shanghai University, Shanghai, 200072, PR China.
authors.forename: Yuanwei
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Key Laboratory of Magnetic Materials and Devices & Key Laboratory of Additive Manufacturing Materials of Zhejiang Province & Division of Functional Materials and Nanodevices, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, PR China.
authors.forename: Ruifen
authors.initials: R
authors.lastname: Zou
authors.affiliation: Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of Education, Chemical Biology Key Laboratory of Hebei Province, College of Chemistry & Environmental Science, Hebei University, Baoding, 071002, PR China.
authors.forename: Jinchao
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Medical Imaging, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, 210002, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Department of Medical Imaging, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, 210002, PR China.
authors.forename: Zhaogang
authors.initials: Z
authors.lastname: Teng
authors.affiliation: Department of Medical Imaging, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, 210002, PR China.
authors.forename: Shouju
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Laboratory of Magnetic Materials and Devices & Key Laboratory of Additive Manufacturing Materials of Zhejiang Province & Division of Functional Materials and Nanodevices, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, PR China.
authors.forename: Wenzhi
authors.initials: W
authors.lastname: Ren
authors.affiliation: Laboratory for Microstructures, Institute of Materials, Shanghai University, Shanghai, 200072, PR China.
authors.forename: Xueshan
authors.initials: X
authors.lastname: Xiao
authors.affiliation: Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, 201204, PR China.
authors.forename: Jichao
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, 201204, PR China.
authors.forename: Lili
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, 201204, PR China.
authors.forename: Aiguo
authors.initials: A
authors.lastname: Li
authors.affiliation: Department of Medical Imaging, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, 210002, PR China. Electronic address: cjr.luguangming@vip.163.com.
authors.forename: Guangming
authors.initials: G
authors.lastname: Lu
authors.affiliation: Key Laboratory of Magnetic Materials and Devices & Key Laboratory of Additive Manufacturing Materials of Zhejiang Province & Division of Functional Materials and Nanodevices, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, PR China. Electronic address: aiguo@nimte.ac.cn.
authors.forename: Aiguo
authors.initials: A
authors.lastname: Wu
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000000981:diagnostic imaging* / Q000188:drug therapy* / Q000378:metabolism; D045744:Cell Line, Tumor; D002735:Chlorophyllides; D003287:Contrast Media / Q000138:chemical synthesis; D005260:Female; D006358:Hot Temperature; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D053769:Nanocapsules / Q000008:administration & dosage / Q000737:chemistry*; D053761:Nanocomposites / Q000627:therapeutic use* / Q000648:ultrastructure; D010778:Photochemotherapy / Q000379:methods*; D017319:Photosensitizing Agents / Q000008:administration & dosage; D011166:Porphyrins / Q000008:administration & dosage*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism; D000068936:Theranostic Nanomedicine / Q000379:methods; D016896:Treatment Outcome
chemical_list: D002735:Chlorophyllides; D003287:Contrast Media; D053769:Nanocapsules; D017319:Photosensitizing Agents; D011166:Porphyrins; D018719:Erb-b2 Receptor Tyrosine Kinases; C062985:phytochlorin; C508053:ERBB2 protein, human
keywords: 808 nm-excited upconversion nanoprobes; HER2-overexpressed breast cancer; High-efficacy photodynamic therapy; Low heating effect