title: Cysteine redox proteomics of the hemoglobin-depleted cytosolic fraction of stored red blood cells.
abstract: Erythrocyte concentrates (ECs) represent the most transfused labile blood products. They are stored at 4°C in additive solutions for up to 56 days. Protein oxidation is a marker of oxidative stress and cysteine residues, whose oxidations are required for physiological cell functions, are highly prone to such modification.
  
  
  
  Five ECs from independent donations were followed. Soluble protein extracts were prepared at days 6, 27, and 41, and cysteines were alkylated, reduced, and labeled with infrared dyes. Samples were mixed two by two (day 6 as reference) and analyzed by 2D-DIGE. Detection of labeled cysteines allows quantitative comparison of oxidative status. Spots of interest were analyzed by proteomics.
  
  
  
  Thirty-two spots containing 43 proteins were classified as increasing, decreasing, or exhibiting a peak of expression during storage. Proteins having catalytic and antioxidant activities were particularly affected during storage, for example, peroxiredoxin-1 and DJ-1 were reversibly oxidized and catalase was irreversibly oxidized. These proteins could be used to evaluate different storage strategies to maintain proper protein function during the overall storage period.
  
  
  
  This redox-DIGE approach brings new quantitative data on oxidized proteins in stored red blood cells. As previously reported on carbonylation, the oxidative damages differently affect protein functions.
authors.affiliation: Laboratoire de Recherche sur les Produits Sanguins, Transfusion Interrégionale CRS, Epalinges, Switzerland.
authors.forename: Julien
authors.initials: J
authors.lastname: Delobel
authors.affiliation: Laboratoire de Recherche sur les Produits Sanguins, Transfusion Interrégionale CRS, Epalinges, Switzerland.
authors.forename: Michel
authors.initials: M
authors.lastname: Prudent
authors.affiliation: Laboratoire de Recherche sur les Produits Sanguins, Transfusion Interrégionale CRS, Epalinges, Switzerland.
authors.forename: David
authors.initials: D
authors.lastname: Crettaz
authors.affiliation: Centre des Neurosciences Psychiatriques, Hôpital de Cery-CHUV, Prilly, Switzerland.
authors.forename: Zeinab
authors.initials: Z
authors.lastname: ElHajj
authors.affiliation: Centre des Neurosciences Psychiatriques, Hôpital de Cery-CHUV, Prilly, Switzerland.
authors.forename: Beat M
authors.initials: BM
authors.lastname: Riederer
authors.affiliation: Laboratoire de Recherche sur les Produits Sanguins, Transfusion Interrégionale CRS, Epalinges, Switzerland.
authors.forename: Jean-Daniel
authors.initials: JD
authors.lastname: Tissot
authors.affiliation: Laboratoire de Recherche sur les Produits Sanguins, Transfusion Interrégionale CRS, Epalinges, Switzerland.
authors.forename: Niels
authors.initials: N
authors.lastname: Lion
mesh_terms: D016477:Artifacts; D001793:Blood Preservation; D001798:Blood Proteins / Q000737:chemistry / Q000378:metabolism*; D001800:Blood Specimen Collection; D003545:Cysteine / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism*; D004912:Erythrocytes / Q000166:cytology* / Q000378:metabolism; D006454:Hemoglobins / Q000302:isolation & purification*; D006801:Humans; D010084:Oxidation-Reduction; D040901:Proteomics; D012995:Solubility; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013997:Time Factors
chemical_list: D001798:Blood Proteins; D006454:Hemoglobins; D013438:Sulfhydryl Compounds; D003545:Cysteine
keywords: Cysteine; Erythrocyte concentrate; Oxidation; Proteomics; Storage