title: Surface-Adsorbed Long G-Quadruplex Nanowires Formed by G:C Linkages.
abstract: G-quadruplexes connected into long, continuous nanostructures termed G-wires show properties superior to dsDNA when applied in nanotechnology. Using AFM imaging, we systematically studied surface adsorption of a set of G-rich oligonucleotides with GC-termini for their ability to form long G-wires through G:C pairing. We investigated the effects of increasing sequence length, the type of nucleotide in the side loops, and removal of the CG-3' terminus. We found that sequences with adenine in the side loops most readily form G-wires. The role of magnesium as an efficient surface-anchoring ion was also confirmed. Conversely, as resolved from dynamic light scattering measurements, magnesium had no ability to promote G-quadruplex formation in solution. These insights may help in selecting prosperous candidates for construction of G-quadruplex based nanowires and to explore them for their electronic properties.
authors.affiliation: Faculty of Mathematics and Physics, University of Ljubljana , Jadranska 19, 1000 Ljubljana, Slovenia.
authors.forename: Tinkara
authors.initials: T
authors.lastname: Troha
authors.affiliation: Faculty of Mathematics and Physics, University of Ljubljana , Jadranska 19, 1000 Ljubljana, Slovenia.|Department of Complex Matter, "Jožef Stefan" Institute , Jamova 39, 1000 Ljubljana, Slovenia.
authors.forename: Irena
authors.initials: I
authors.lastname: Drevenšek-Olenik
authors.affiliation: Biomedical Sciences Research Institute, University of Ulster , Coleraine BT51 2SA, United Kingdom.
authors.forename: Mateus
authors.initials: M
authors.lastname: Webba da Silva
authors.affiliation: Department of Complex Matter, "Jožef Stefan" Institute , Jamova 39, 1000 Ljubljana, Slovenia.|Faculty of Mechanical Engineering, University of Maribor , Smetanova 17, 2000 Maribor, Slovenia.
authors.forename: Lea
authors.initials: L
authors.lastname: Spindler