title: Circulating SIRT1 inversely correlates with epicardial fat thickness in patients with obesity.
abstract: Obesity is increasing worldwide and is related to undesirable cardiovascular outcomes. Epicardial fat (EF), the heart visceral fat depot, increases with obesity and correlates with cardiovascular risk. SIRT1, an enzyme regulating metabolic circuits linked with obesity, has a cardioprotective effect and is a predictor of cardiovascular events. We aimed to assess the relationship of EF thickness (EFT) with circulating SIRT1 in patients with obesity.
  
  
  
  Sixty-two patients affected by obesity and 23 lean controls were studied. Plasma SIRT1 concentration was determined by enzyme-linked immunosorbent assay (ELISA). EFT was measured by echocardiography. Body mass index (BMI), waist circumference, heart rate (HR), blood pressure, and laboratory findings (fasting glucose, insulin, HbA1c, cholesterol, and triglycerides) were assessed. SIRT1 was significantly lower (P = 0.002) and EFT was higher (P < 0.0001) in patients with obesity compared with lean controls. SIRT1 showed a negative correlation with EFT and HR in the obesity group (ρ = -0.350, P = 0.005; ρ = -0.303, P = 0.008, respectively). After adjustment for obesity-correlated variables, multiple linear regression analysis showed that EFT remained the best correlate of SIRT1 (β = -0.352, P = 0.016).
  
  
  
  Circulating SIRT1 correlates with the visceral fat content of the heart. Serum SIRT1 levels might provide additional information for risk assessment of coronary artery disease in patients with obesity.
authors.affiliation: Department of Experimental Medicine, Section of Medical Physiopathology, Food Science and Endocrinology, "Sapienza" University of Rome, 00161 Rome, Italy. Electronic address: stefaniamariani@yahoo.com.
authors.forename: S
authors.initials: S
authors.lastname: Mariani
authors.affiliation: Department of Experimental Medicine, Section of Medical Physiopathology, Food Science and Endocrinology, "Sapienza" University of Rome, 00161 Rome, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Costantini
authors.affiliation: Department of Experimental Medicine, Section of Medical Physiopathology, Food Science and Endocrinology, "Sapienza" University of Rome, 00161 Rome, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Lubrano
authors.affiliation: Department of Experimental Medicine, Section of Medical Physiopathology, Food Science and Endocrinology, "Sapienza" University of Rome, 00161 Rome, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Basciani
authors.affiliation: Department of Experimental Medicine, Section of Medical Physiopathology, Food Science and Endocrinology, "Sapienza" University of Rome, 00161 Rome, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Caldaroni
authors.affiliation: Department of Experimental Medicine, Section of Medical Physiopathology, Food Science and Endocrinology, "Sapienza" University of Rome, 00161 Rome, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Barbaro
authors.affiliation: Department of Experimental Medicine, Section of Medical Physiopathology, Food Science and Endocrinology, "Sapienza" University of Rome, 00161 Rome, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Poggiogalle
authors.affiliation: Department of Experimental Medicine, Section of Medical Physiopathology, Food Science and Endocrinology, "Sapienza" University of Rome, 00161 Rome, Italy.
authors.forename: L M
authors.initials: LM
authors.lastname: Donini
authors.affiliation: Department of Experimental Medicine, Section of Medical Physiopathology, Food Science and Endocrinology, "Sapienza" University of Rome, 00161 Rome, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Lenzi
authors.affiliation: Department of Experimental Medicine, Section of Medical Physiopathology, Food Science and Endocrinology, "Sapienza" University of Rome, 00161 Rome, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Gnessi
mesh_terms: D050154:Adiposity; D000293:Adolescent; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D003324:Coronary Artery Disease / Q000097:blood / Q000201:enzymology / Q000209:etiology* / Q000503:physiopathology; D015150:Echocardiography, Doppler; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D050152:Intra-Abdominal Fat / Q000000981:diagnostic imaging / Q000503:physiopathology*; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009765:Obesity / Q000097:blood* / Q000150:complications / Q000201:enzymology / Q000503:physiopathology; D010496:Pericardium / Q000000981:diagnostic imaging / Q000503:physiopathology*; D018570:Risk Assessment; D012307:Risk Factors; D056564:Sirtuin 1 / Q000097:blood*; D055815:Young Adult
chemical_list: D015415:Biomarkers; C447939:SIRT1 protein, human; D056564:Sirtuin 1
keywords: Epicardial fat; Obesity; SIRT1