title: Multilocus sequence typing of Mycoplasma mycoides subsp. capri to assess its genetic variability in a contagious agalactia endemic area.
abstract: Mycoplasma mycoides subsp. capri (Mmc) is one of the main causative agents of caprine contagious agalactia. Besides, the absence of accurate control methods eases its dispersion between different herds within endemic areas of this disease. In this context, there is a need to implement molecular typing schemes which offer valuable information useful to establish control measures and enables the surveillance of this pathogen. The aim of this study was to assess the genetic variability of different strains of Mmc from a contagious agalactia endemic area through multilocus sequence typing (MLST). For this purpose, five house-keeping genes (fusA, glpQ, gyrB, lepA, rpoB) from 39 field isolates were analysed. These isolates were obtained from different geographic areas of Spain, between the years 2004 and 2015. The results obtained in this study suggest that the selected MLST scheme could be a useful technique to monitor the genetic variability of Mmc in endemic areas. Despite the significant differences found between the assessed field isolates, they could be classified according to their geographical origin. Moreover, it was also possible to detect genetic differences between Mmc strains coming from the same herd at the same sampling time, which may need to be taken into consideration when designing or arranging prophylactic strategies.
authors.affiliation: Ruminant Health Research Group, Department of Animal Health, Faculty of Veterinary Sciences, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, Campus de Espinardo s/n., 30100 Murcia, Spain.
authors.forename: Juan
authors.initials: J
authors.lastname: Tatay-Dualde
authors.affiliation: Ruminant Health Research Group, Department of Animal Health, Faculty of Veterinary Sciences, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, Campus de Espinardo s/n., 30100 Murcia, Spain.
authors.forename: Miranda
authors.initials: M
authors.lastname: Prats-van der Ham
authors.affiliation: Ruminant Health Research Group, Department of Animal Health, Faculty of Veterinary Sciences, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, Campus de Espinardo s/n., 30100 Murcia, Spain. Electronic address: cdelafe@um.es.
authors.forename: Christian
authors.initials: C
authors.lastname: de la Fe
authors.affiliation: Ruminant Health Research Group, Department of Animal Health, Faculty of Veterinary Sciences, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, Campus de Espinardo s/n., 30100 Murcia, Spain.
authors.forename: Ángel
authors.initials: Á
authors.lastname: Gómez-Martín
authors.affiliation: Ruminant Health Research Group, Department of Animal Health, Faculty of Veterinary Sciences, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, Campus de Espinardo s/n., 30100 Murcia, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Paterna
authors.affiliation: Ruminant Health Research Group, Department of Animal Health, Faculty of Veterinary Sciences, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, Campus de Espinardo s/n., 30100 Murcia, Spain.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Corrales
authors.affiliation: Ruminant Health Research Group, Department of Animal Health, Faculty of Veterinary Sciences, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, Campus de Espinardo s/n., 30100 Murcia, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Contreras
authors.affiliation: Ruminant Health Research Group, Department of Animal Health, Faculty of Veterinary Sciences, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, Campus de Espinardo s/n., 30100 Murcia, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Sánchez
mesh_terms: D000818:Animals; D019353:Endemic Diseases; D005798:Genes, Bacterial / Q000235:genetics; D014644:Genetic Variation; D015511:Goat Diseases / Q000453:epidemiology / Q000382:microbiology*; D006041:Goats; D058885:Multilocus Sequence Typing; D009175:Mycoplasma Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D009176:Mycoplasma mycoides / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D013030:Spain / Q000453:epidemiology
keywords: Contagious agalactia; Epidemiology; Goat; Multilocus sequence typing; Mycoplasma mycoides subsp. capri; Surveillance