title: Cytotoxic cardenolides and sesquiterpenoids from the fruits of Reevesia formosana.
abstract: Bioassay-guided fractionation of the fruits of Reevesia formosana led to isolation of three cardenolides (reevesioside J, reevesioside K, and epi-reevesioside K), three sesquiterpenoids (reevesiterpenol C, reevesiterpenol D, and reevesiterpenol E), and two glycosides (reevesianin A and reevesianin B), along with 46 known compounds. Their structures were determined using spectroscopic techniques. In addition to the reported cytotoxic cardenolides, reevesioside J and strophanthidin exhibited moderate cytotoxicity against the cell lines MCF-7, NCI-H460, and HepG2, with IC50 values of 0.39 ± 0.06 μM and 1.06 ± 0.12 μM for MCF-7, 0.12 ± 0.01 μM and 0.29 ± 0.01 μM for NCI-H460, and 1.09 ± 0.02 μM and 1.72 ± 0.02 μM for HepG2, respectively. Reevesiterpenol E also exhibited the best selective cytotoxicity to the NCI-H460 cell line, with an IC50 value of 3.15 ± 0.22 μM.
authors.affiliation: Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, 807, Taiwan, ROC.
authors.forename: Pei-Yu
authors.initials: PY
authors.lastname: Hsiao
authors.affiliation: Institute of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Miaoli, 350, Taiwan, ROC.
authors.forename: Shiow-Ju
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, 807, Taiwan, ROC; School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, 807, Taiwan, ROC; Research Center for Natural Products and Drug Development, Kaohsiung Medical University, Kaohsiung, 807, Taiwan, ROC.
authors.forename: Ih-Sheng
authors.initials: IS
authors.lastname: Chen
authors.affiliation: Institute of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Miaoli, 350, Taiwan, ROC.
authors.forename: Hsing-Yu
authors.initials: HY
authors.lastname: Hsu
authors.affiliation: Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, 807, Taiwan, ROC; School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, 807, Taiwan, ROC; Research Center for Natural Products and Drug Development, Kaohsiung Medical University, Kaohsiung, 807, Taiwan, ROC; Center for Infectious Disease and Cancer Research (CICAR), Kaohsiung Medical University, Kaohsiung, 807, Taiwan, ROC. Electronic address: hschang@kmu.edu.tw.
authors.forename: Hsun-Shuo
authors.initials: HS
authors.lastname: Chang
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002298:Cardenolides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D005260:Female; D005638:Fruit / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D056945:Hep G2 Cells; D006801:Humans; D061986:MCF-7 Cells; D019660:Malvaceae / Q000737:chemistry*; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D054830:Sesterterpenes
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D002298:Cardenolides; D006027:Glycosides; D012717:Sesquiterpenes; D054830:Sesterterpenes; C000614071:reevesioside J; C000614072:reevesioside K; C000614073:reevesiterpenol C; C000614075:reevesiterpenol D; C000614074:reevesiterpenol E
keywords: Cardenolides; Cytotoxicity; Reevesia formosana; Sesquiterpenoids; Sterculiaceae