title: UCP2 and PLIN1 Expression Affects the Resting Metabolic Rate and Weight Loss on Obese Patients.
abstract: Analysis of the expression of genes related to the energy metabolism can elucidate the understanding of physiological and genetic factors that contribute to obesity. This study aimed to evaluate the expression of genes and its influence on resting metabolic rate and weight loss in obese patients before and after bariatric surgery.
  
  
  
  This study was conducted on 23 women, who were divided into two groups: bariatric surgery (preoperative and 6 months after surgery) and control. Abdominal subcutaneous adipose tissue samples were collected to analyze the gene expression, and the resting metabolic rate (RMR) was measured by indirect calorimetry.
  
  
  
  Significant differences were observed in weight reduction (22 %, p = 0.01), BMI (22.5 %, p = 0.01), and RMR values (10.5 %, p = 0.01) after the bariatric surgery, while the weight-adjusted RMR increased (15.8 %, p = 0.01). Increased UCP2 expression after 6 months of Roux-en-Y gastric bypass (RYGB) as compared to preoperative period (0.764 to 1.268, p = 0.01) was observed. Analysis with weight-adjusted RMR as dependent variable revealed that UCP2 (r2= 0.517, p = 0.01) and PLIN1 (r2= 0.420, p = 0.04) expression determine the RMR values in preoperative period. Moreover, UCP2 and PLIN1 expression in preoperative period influenced the percentage of weight loss, even when adjusted for age and BMI.
  
  
  
  We have demonstrated that after 6 months of bariatric surgery, there is significant increase in the UCP2 expression. Additionally, the expression of UCP2 and PLIN1 genes influences the resting metabolic rate in obese individuals and could predict the weight loss after bariatric surgery.
authors.affiliation: Department of Internal Medicine, Faculty of Medicine of Ribeirao Preto, University of São Paulo, Avenida dos Bandeirantes, 3900, Ribeirão Preto, SP, 14049-900, Brazil.
authors.forename: Bruno Affonso Parenti
authors.initials: BAP
authors.lastname: de Oliveira
authors.affiliation: Department of Internal Medicine, Faculty of Medicine of Ribeirao Preto, University of São Paulo, Avenida dos Bandeirantes, 3900, Ribeirão Preto, SP, 14049-900, Brazil.
authors.forename: Marcela Augusta
authors.initials: MA
authors.lastname: de Souza Pinhel
authors.affiliation: Department of Internal Medicine, Faculty of Medicine of Ribeirao Preto, University of São Paulo, Avenida dos Bandeirantes, 3900, Ribeirão Preto, SP, 14049-900, Brazil.
authors.forename: Carolina Ferreira
authors.initials: CF
authors.lastname: Nicoletti
authors.affiliation: Department of Internal Medicine, Faculty of Medicine of Ribeirao Preto, University of São Paulo, Avenida dos Bandeirantes, 3900, Ribeirão Preto, SP, 14049-900, Brazil.
authors.forename: Cristiana Cortes
authors.initials: CC
authors.lastname: de Oliveira
authors.affiliation: Department of Internal Medicine, Faculty of Medicine of Ribeirao Preto, University of São Paulo, Avenida dos Bandeirantes, 3900, Ribeirão Preto, SP, 14049-900, Brazil.
authors.forename: Driele Cristina Gomes
authors.initials: DCG
authors.lastname: Quinhoneiro
authors.affiliation: Department of Internal Medicine, Faculty of Medicine of Ribeirao Preto, University of São Paulo, Avenida dos Bandeirantes, 3900, Ribeirão Preto, SP, 14049-900, Brazil.
authors.forename: Natália Yumi
authors.initials: NY
authors.lastname: Noronha
authors.affiliation: Department of Internal Medicine, Faculty of Medicine of Ribeirao Preto, University of São Paulo, Avenida dos Bandeirantes, 3900, Ribeirão Preto, SP, 14049-900, Brazil.
authors.forename: Priscila Giácomo
authors.initials: PG
authors.lastname: Fassini
authors.affiliation: Department of Internal Medicine, Faculty of Medicine of Ribeirao Preto, University of São Paulo, Avenida dos Bandeirantes, 3900, Ribeirão Preto, SP, 14049-900, Brazil.
authors.forename: Wilson Araújo
authors.initials: WA
authors.lastname: da Silva Júnior
authors.affiliation: Department of Internal Medicine, Faculty of Medicine of Ribeirao Preto, University of São Paulo, Avenida dos Bandeirantes, 3900, Ribeirão Preto, SP, 14049-900, Brazil.
authors.forename: Wilson Salgado
authors.initials: WS
authors.lastname: Junior
authors.affiliation: Department of Internal Medicine, Faculty of Medicine of Ribeirao Preto, University of São Paulo, Avenida dos Bandeirantes, 3900, Ribeirão Preto, SP, 14049-900, Brazil. carla@fmrp.usp.br.
authors.forename: Carla Barbosa
authors.initials: CB
authors.lastname: Nonino
mesh_terms: D000328:Adult; D050110:Bariatric Surgery; D001481:Basal Metabolism / Q000235:genetics*; D016022:Case-Control Studies; D004734:Energy Metabolism / Q000235:genetics; D005260:Female; D006801:Humans; D008875:Middle Aged; D009767:Obesity, Morbid / Q000235:genetics* / Q000378:metabolism / Q000601:surgery; D000070778:Perilipin-1 / Q000235:genetics* / Q000378:metabolism; D057234:Preoperative Period; D050151:Subcutaneous Fat / Q000378:metabolism; D016896:Treatment Outcome; D000071246:Uncoupling Protein 2 / Q000235:genetics* / Q000378:metabolism; D015431:Weight Loss / Q000235:genetics*; D055815:Young Adult
chemical_list: C000602978:PLIN1 protein, human; D000070778:Perilipin-1; C000604389:UCP2 protein, human; D000071246:Uncoupling Protein 2
keywords: Gene expression; Obesity; Resting metabolic rate