title: Enterococcus saigonensis sp. nov., isolated from retail chicken meat and liver.
abstract: Two Gram-stain-positive strains, VE80T and VE116, which were resistant to vancomycin, were isolated from retail chicken meat and liver in Ho Chi Minh, Vietnam, respectively. These strains were characterized by sequence analyses of 16S rRNA, RNA polymerase α-subunit (rpoA), ATP synthase α-subunit (atpA), and phenylalanyl-tRNA synthase α-subunit (pheS) genes, determination of DNA G+C content, cellular fatty acid methyl ester analysis, DNA-DNA hybridization, and conventional morphological and biochemical tests. Strains VE80T and VE116 had 99.6 % 16S rRNA gene sequence similarity with Enterococcus canintestini LMG 13590T, and 99.1 % 16S rRNA gene sequence similarity with Enterococcus dispar ATCC 51266T. However, the two isolates could be clearly differentiated from these reference strains by the low sequence similarities (86.1-86.8 %) of the atpA gene, low DNA-DNA relatedness (<22.8 %), and differences in the production of acid from melezitose and methyl α-d-glucoside. Based on the results obtained in the present study, these two isolates are considered to represent a novel species of the genus Enterococcus, for which the name Enterococcus saigonensis sp. nov., is proposed. The type strain is VE80T (=JCM 31193T=CCUG 68827T).
authors.affiliation: Osaka Prefectural Institute of Public Health, Osaka 537-0025, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Harada
authors.affiliation: Institute of Public Health, 159 Hung Phu, District 8, Ward 8, Ho Chi Minh City, Vietnam.
authors.forename: Van Chinh
authors.initials: VC
authors.lastname: Dang
authors.affiliation: Institute of Public Health, 159 Hung Phu, District 8, Ward 8, Ho Chi Minh City, Vietnam.
authors.forename: Do Phuc
authors.initials: DP
authors.lastname: Nguyen
authors.affiliation: Institute of Public Health, 159 Hung Phu, District 8, Ward 8, Ho Chi Minh City, Vietnam.
authors.forename: Thi Anh Dao
authors.initials: TAD
authors.lastname: Nguyen
authors.affiliation: Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Tsukuba, Ibaraki 305-0074, Japan.
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Sakamoto
authors.affiliation: Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Tsukuba, Ibaraki 305-0074, Japan.
authors.forename: Moriya
authors.initials: M
authors.lastname: Ohkuma
authors.affiliation: Department of Infection Metagenomics, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Motooka
authors.affiliation: Department of Infection Metagenomics, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan.
authors.forename: Shota
authors.initials: S
authors.lastname: Nakamura
authors.affiliation: Osaka Prefectural Institute of Public Health, Osaka 537-0025, Japan.
authors.forename: Kotaro
authors.initials: K
authors.lastname: Uchida
authors.affiliation: Osaka Prefectural Institute of Public Health, Osaka 537-0025, Japan.
authors.forename: Michio
authors.initials: M
authors.lastname: Jinnai
authors.affiliation: Osaka Prefectural Institute of Public Health, Osaka 537-0025, Japan.
authors.forename: Shinya
authors.initials: S
authors.lastname: Yonogi
authors.affiliation: Osaka Prefectural Institute of Public Health, Osaka 537-0025, Japan.
authors.forename: Ryuji
authors.initials: R
authors.lastname: Kawahara
authors.affiliation: Osaka Prefectural Institute of Public Health, Osaka 537-0025, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Kanki
authors.affiliation: Osaka Prefectural Institute of Public Health, Osaka 537-0025, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Kawai
authors.affiliation: Osaka Prefectural Institute of Public Health, Osaka 537-0025, Japan.
authors.forename: Yuko
authors.initials: Y
authors.lastname: Kumeda
authors.affiliation: Osaka Prefectural Institute of Public Health, Osaka 537-0025, Japan.
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002645:Chickens / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D016983:Enterococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D005227:Fatty Acids / Q000737:chemistry; D008099:Liver / Q000382:microbiology*; D008460:Meat / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014744:Vietnam
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012321:DNA-Directed RNA Polymerases; C113275:RNA polymerase alpha subunit