title: Evaluation of a novel saliva-based epidermal growth factor receptor mutation detection for lung cancer: A pilot study.
abstract: BACKGROUND
  This article describes a pilot study evaluating a novel liquid biopsy system for non-small cell lung cancer (NSCLC) patients. The electric field-induced release and measurement (EFIRM) method utilizes an electrochemical biosensor for detecting oncogenic mutations in biofluids.
  
  
  METHODS
  Saliva and plasma of 17 patients were collected from three cancer centers prior to and after surgical resection. The EFIRM method was then applied to the collected samples to assay for exon 19 deletion and p.L858 mutations. EFIRM results were compared with cobas results of exon 19 deletion and p.L858 mutation detection in cancer tissues.
  
  
  RESULTS
  The EFIRM method was found to detect exon 19 deletion with an area under the curve (AUC) of 1.0 in both saliva and plasma samples in lung cancer patients. For L858R mutation detection, the AUC of saliva was 1.0, while the AUC of plasma was 0.98. Strong correlations were also found between presurgery and post-surgery samples for both saliva (0.86 for exon 19 and 0.98 for L858R) and plasma (0.73 for exon 19 and 0.94 for L858R).
  
  
  CONCLUSIONS
  Our study demonstrates the feasibility of utilizing EFIRM to rapidly, non-invasively, and conveniently detect epidermal growth factor receptor mutations in the saliva of patients with NSCLC, with results corresponding perfectly with the results of cobas tissue genotyping.
authors.affiliation: Lung Cancer Center, West China Hospital Sichuan University Chengdu China.
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authors.lastname: Pu
authors.affiliation: Lung Cancer Center, West China Hospital Sichuan University Chengdu China.
authors.forename: Hao
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authors.lastname: Liang
authors.affiliation: School of Dentistry, Department of Pathology University of California Los Angeles California USA.
authors.forename: Fang
authors.initials: F
authors.lastname: Wei
authors.affiliation: School of Dentistry, Department of Pathology University of California Los Angeles California USA.
authors.forename: David
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authors.lastname: Akin
authors.affiliation: Department of Biostatistics, MD Anderson Cancer Center University of Texas Houston Texas USA.
authors.forename: Ziding
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authors.lastname: Feng
authors.affiliation: Department of Biostatistics, MD Anderson Cancer Center University of Texas Houston Texas USA.
authors.forename: QingXiang
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authors.lastname: Yan
authors.affiliation: Department of Thoracic Surgery, Henan Cancer Hospital University of Zhengzhou Zhengzhou China.
authors.forename: Yin
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authors.lastname: Li
authors.affiliation: Department of Thoracic Surgery, Henan Cancer Hospital University of Zhengzhou Zhengzhou China.
authors.forename: Yan
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authors.affiliation: Department of Thoracic Surgery, Jiangsu Cancer Hospital Nanjing Medical University Nanjing China.
authors.forename: Lin
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authors.affiliation: Department of Thoracic Surgery, Jiangsu Cancer Hospital Nanjing Medical University Nanjing China.
authors.forename: Gaochao
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authors.lastname: Dong
authors.affiliation: Laboratory of Lung Development and Disease West China Second University Hospital, Sichuan University Chengdu China.
authors.forename: Huajing
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authors.lastname: Wan
authors.affiliation: Lung Cancer Center, West China Hospital Sichuan University Chengdu China.
authors.forename: Jingsi
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authors.lastname: Dong
authors.affiliation: Lung Cancer Center, West China Hospital Sichuan University Chengdu China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Qiu
authors.affiliation: Lung Cancer Center, West China Hospital Sichuan University Chengdu China.
authors.forename: Changlong
authors.initials: C
authors.lastname: Qin
authors.affiliation: Lung Cancer Center, West China Hospital Sichuan University Chengdu China.
authors.forename: Daxing
authors.initials: D
authors.lastname: Zhu
authors.affiliation: Lung Cancer Center, West China Hospital Sichuan University Chengdu China.
authors.forename: Xi
authors.initials: X
authors.lastname: Wang
authors.affiliation: Lung Cancer Center, West China Hospital Sichuan University Chengdu China.
authors.forename: Tong
authors.initials: T
authors.lastname: Sun
authors.affiliation: Lung Cancer Center, West China Hospital Sichuan University Chengdu China.
authors.forename: Wenbiao
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Lung Cancer Center, West China Hospital Sichuan University Chengdu China.
authors.forename: Canjun
authors.initials: C
authors.lastname: Li
authors.affiliation: Lung Cancer Center, West China Hospital Sichuan University Chengdu China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Tang
authors.affiliation: Department of Cancer Epidemiology Cancer Hospital/Institute, Chinese Academy of Medical Sciences Beijing China.
authors.forename: Youlin
authors.initials: Y
authors.lastname: Qiao
authors.affiliation: School of Dentistry, Department of Pathology University of California Los Angeles California USA.
authors.forename: David T W
authors.initials: DT
authors.lastname: Wong
authors.affiliation: Lung Cancer Center, West China Hospital Sichuan University Chengdu China.
authors.forename: Qinghua
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authors.lastname: Zhou
keywords: EGFR mutation; liquid biopsy; lung cancer; saliva diagnostics