title: Impaired Recall of Positional Memory following Chemogenetic Disruption of Place Field Stability.
abstract: The neural network of the temporal lobe is thought to provide a cognitive map of our surroundings. Functional analysis of this network has been hampered by coarse tools that often result in collateral damage to other circuits. We developed a chemogenetic system to temporally control electrical input into the hippocampus. When entorhinal input to the perforant path was acutely silenced, hippocampal firing patterns became destabilized and underwent extensive remapping. We also found that spatial memory acquired prior to neural silencing was impaired by loss of input through the perforant path. Together, our experiments show that manipulation of entorhinal activity destabilizes spatial coding and disrupts spatial memory. Moreover, we introduce a chemogenetic model for non-invasive neuronal silencing that offers multiple advantages over existing strategies in this setting.
authors.affiliation: Department of Neuroscience, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: Rong
authors.initials: R
authors.lastname: Zhao
authors.affiliation: Department of Neuroscience, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: Stacy D
authors.initials: SD
authors.lastname: Grunke
authors.affiliation: Department of Neuroscience, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: Madhusudhanan M
authors.initials: MM
authors.lastname: Keralapurath
authors.affiliation: Department of Neuroscience, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Yetman
authors.affiliation: Department of Neuroscience, Baylor College of Medicine, Houston, TX 77030, USA; Department of Cognitive Science, Rice University, Houston, TX 77251, USA.
authors.forename: Alexander
authors.initials: A
authors.lastname: Lam
authors.affiliation: Department of Neuroscience, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: Tang-Cheng
authors.initials: TC
authors.lastname: Lee
authors.affiliation: Department of Neuroscience, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Sousounis
authors.affiliation: Institute for Applied Cancer Science, The University of Texas MD Anderson Cancer Center, Houston, TX 77054, USA.
authors.forename: Yongying
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Mouse Cancer Genetics Program, Center for Cancer Research, National Cancer Institute, Frederick, MD 21702, USA.
authors.forename: Deborah A
authors.initials: DA
authors.lastname: Swing
authors.affiliation: Mouse Cancer Genetics Program, Center for Cancer Research, National Cancer Institute, Frederick, MD 21702, USA.
authors.forename: Lino
authors.initials: L
authors.lastname: Tessarollo
authors.affiliation: Department of Neuroscience, Baylor College of Medicine, Houston, TX 77030, USA; Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA. Electronic address: dji@bcm.edu.
authors.forename: Daoyun
authors.initials: D
authors.lastname: Ji
authors.affiliation: Department of Neuroscience, Baylor College of Medicine, Houston, TX 77030, USA; Department of Neurology, Baylor College of Medicine, Houston, TX 77030, USA; Department of Neurosurgery, Baylor College of Medicine, Houston, TX 77030, USA; Huffington Center on Aging, Baylor College of Medicine, Houston, TX 77030, USA. Electronic address: jankowsk@bcm.edu.
authors.forename: Joanna L
authors.initials: JL
authors.lastname: Jankowsky
mesh_terms: D000818:Animals; D018728:Entorhinal Cortex / Q000502:physiology; D005260:Female; D006624:Hippocampus / Q000502:physiology*; D006801:Humans; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008959:Models, Neurological; D009415:Nerve Net / Q000502:physiology*; D019580:Perforant Pathway / Q000502:physiology; D065852:Spatial Memory / Q000502:physiology*; D013702:Temporal Lobe / Q000502:physiology*