title: Biological devulcanization of ground natural rubber by Gordonia desulfuricans DSM 44462(T) strain.
abstract: Due to the rapid increase of waste vulcanized rubber products, the development of low-cost, efficient, and selective devulcanization processes is needed. In this paper, the devulcanization ability of Gordonia desulfuricans DSM 44462(T) was evaluated by a design of experiments. The aim of the experimental design was to investigate the importance of parameters influencing the bacterial growth, such as the glucose concentration (C), dibenzothiophene concentration (DBT), and initial biomass (optical density, OD) in biodevulcanization process. The complex viscosity (η*) was chosen as experimental response for the experimental design. A multiple linear regression was used to model the relationship between the response and the process variables. In addition, the crosslink density and gel fraction were measured. Furthermore, the automated ribosomal intergenic spacer analysis (ARISA) as a microbiological method was performed to assess the persistence of the inoculated strain during the experiments. Reduced regression models were obtained considering only the significant variables and interactions. The glucose concentration C and OD variables and C-DBT and DBT-OD interactions resulted to the relevant parameters for the process. The fingerprinting showed the persistence of G. desulfuricans DSM 44462(T), despite the presence of other bacterial population after the VGNR sterilization. These results highlight the importance to support the physics analysis with microbiological analyses to evaluate the bacterial persistence during the treatment.
authors.affiliation: Department of Earth and Environmental Sciences, University of Milano-Bicocca, Piazza della Scienza 1, 20126, Milano, Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Tatangelo
authors.affiliation: Department of Earth and Environmental Sciences, University of Milano-Bicocca, Piazza della Scienza 1, 20126, Milano, Italy.
authors.forename: Ivan
authors.initials: I
authors.lastname: Mangili
authors.affiliation: Pirelli Labs S.p.A., Via Piero e Alberto Pirelli 25, 20126, Milano, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Caracino
authors.affiliation: Department of Earth and Environmental Sciences, University of Milano-Bicocca, Piazza della Scienza 1, 20126, Milano, Italy.
authors.forename: Manuela
authors.initials: M
authors.lastname: Anzano
authors.affiliation: Department of Earth and Environmental Sciences, University of Milano-Bicocca, Piazza della Scienza 1, 20126, Milano, Italy.|Department of Microbiology, Faculty of Biology Sciences, University of Shahid Beheshti, Shahid Chamran Highway, Evin, Tehran, Iran.
authors.forename: Ziba
authors.initials: Z
authors.lastname: Najmi
authors.affiliation: Department of Earth and Environmental Sciences, University of Milano-Bicocca, Piazza della Scienza 1, 20126, Milano, Italy.
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Bestetti
authors.affiliation: Department of Earth and Environmental Sciences, University of Milano-Bicocca, Piazza della Scienza 1, 20126, Milano, Italy.
authors.forename: Elena
authors.initials: E
authors.lastname: Collina
authors.affiliation: Department of Earth and Environmental Sciences, University of Milano-Bicocca, Piazza della Scienza 1, 20126, Milano, Italy. andrea.franzetti@unimib.it.
authors.forename: Andrea
authors.identifier: http://orcid.org/0000-0003-1279-9940
authors.initials: A
authors.lastname: Franzetti
authors.affiliation: Department of Earth and Environmental Sciences, University of Milano-Bicocca, Piazza della Scienza 1, 20126, Milano, Italy.
authors.forename: Marina
authors.initials: M
authors.lastname: Lasagni
mesh_terms: D001709:Biotechnology / Q000379:methods; D001711:Biotransformation; D003470:Culture Media / Q000737:chemistry; D033601:Gordonia Bacterium / Q000254:growth & development / Q000378:metabolism*; D012037:Refuse Disposal / Q000379:methods; D012408:Rubber / Q000378:metabolism*
chemical_list: D003470:Culture Media; D012408:Rubber
keywords: ARISA; Biological devulcanization; Gordonia desulfuricans DSM 44462T; Recycling; Vulcanized ground natural rubber