title: Coevolution of neoplastic epithelial cells and multilineage stroma via polyploid giant cells during immortalization and transformation of mullerian epithelial cells.
abstract: Stromal cells are generally considered to be derived primarily from the host's normal mesenchymal stromal cells or bone marrow. However, the origins of stromal cells have been quite controversial. To determine the role of polyploidy in tumor development, we examined the fate of normal mullerian epithelial cells during the immortalization and transformation process by tracing the expression of SV40 large T antigen. Here we show that immortalized or HRAS-transformed mullerian epithelial cells contain a subpopulation of polyploid giant cells that grow as multicellular spheroids expressing hematopoietic markers in response to treatment with CoCl2. The immortalized or transformed epithelial cells can transdifferentiate into stromal cells when transplanted into nude mice. Immunofluorescent staining revealed expression of stem cell factors OCT4, Nanog, and SOX-2 in spheroid, whereas expression of embryonic stem cell marker SSEA1 was increased in HRAS-transformed cells compared with their immortalized isogenic counterparts. These results suggest that normal mullerian epithelial cells are intrinsically highly plastic, via the formation of polyploid giant cells and activation of embryonic stem-like program, which work together to promote the coevolution of neoplastic epithelial cells and multiple lineage stromal cells.
authors.affiliation: Department of Pathology, Tianjin Union Medical Center, Tianjin, P.R. China; Department of Pathology, University of Texas, MD Anderson Cancer Center, Houston, Texas, USA.
authors.forename: Shiwu
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Pathology, University of Texas, MD Anderson Cancer Center, Houston, Texas, USA.
authors.forename: Imelda
authors.initials: I
authors.lastname: Mercado-Uribe
authors.affiliation: Department of Gynecologic Oncology, University of Texas, MD Anderson Cancer Center, Houston, Texas, USA; Department of Cancer Biology, University of Texas, MD Anderson Cancer Center, Houston, Texas, USA; Department of Center for RNA Interference and Non-Coding RNA, University of Texas, MD Anderson Cancer Center, Houston, Texas, USA.
authors.forename: Anil
authors.initials: A
authors.lastname: Sood
authors.affiliation: Department of Experimental Therapeutics, University of Texas, MD Anderson Cancer Center, Houston, Texas, USA.
authors.forename: Robert C
authors.initials: RC
authors.lastname: Bast
authors.affiliation: Department of Pathology, University of Texas, MD Anderson Cancer Center, Houston, Texas, USA; Department of Molecular and Cellular Oncology, University of Texas, MD Anderson Cancer Center, Houston, Texas, USA.
authors.forename: Jinsong
authors.initials: J
authors.lastname: Liu
keywords: RAS; multipotent cancer stem cells; polyploid giant cells; stromal differentiation