title: Crystallographic Studies of Telomerase.
abstract: Telomeres are nucleoprotein complexes that maintain the ends of our chromosomes thus providing genomic stability. Telomerase is a ribonucleoprotein reverse transcriptase that replicates the short tandem repeats of DNA known as telomeres. The telomeric DNA is specifically associated with two major complexes, the shelterin and CST complexes both of which are involved in telomere length regulation and maintenance along with telomerase. Obtaining structural information on these nucleoprotein complexes has been a major bottleneck in fully understanding the mechanism of action of telomeric nucleoproteins for over two decades. The recent advances in molecular and structural biology have enabled us to obtain atomic resolution structures of telomeric proteins alone and in complex with their nucleic acid substrates transforming the field and our understanding and interpretation of this unique biological pathway. Here we report our approach to obtain the structure of the Triobolium castaneum catalytic subunit of telomerase TERT (tcTERT) in its apo- and substrate-bound states.
authors.affiliation: The Wistar Institute, Philadelphia, PA, United States.
authors.forename: H
authors.initials: H
authors.lastname: Hoffman
authors.affiliation: The Wistar Institute, Philadelphia, PA, United States. Electronic address: skorda@wistar.org.
authors.forename: E
authors.initials: E
authors.lastname: Skordalakes
mesh_terms: D000818:Animals; D020134:Catalytic Domain; D018360:Crystallography, X-Ray / Q000379:methods*; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D057075:Enzyme Assays / Q000379:methods; D006801:Humans; D008958:Models, Molecular; D011487:Protein Conformation; D012313:RNA / Q000737:chemistry / Q000378:metabolism; D019098:Telomerase / Q000737:chemistry* / Q000378:metabolism; D014231:Tribolium / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism
chemical_list: D012313:RNA; D004247:DNA; D019098:Telomerase
keywords: Cancer; Reverse transcription; Telomerase; Telomeres