title: Beta Lactams Antibiotic Ceftriaxone Modulates Seizures, Oxidative Stress and Connexin 43 Expression in Hippocampus of Pentylenetetrazole Kindled Rats.
abstract: OBJECTIVE
  This study aimed to investigate the effect of ceftriaxone on oxidative stress and gap junction protein (connexin 43, Cx-43) expression in pentylenetetrazole (PTZ) induced kindling model.
  
  
  METHODS
  Twenty four Sprague dawely rats were divided into 3 equal groups (a) normal group: normal rats. (b) PTZ kindled group: received PTZ at the dose of 50 mg/kg via intraperitoneal injection (i.p.) every other day for 2 weeks (c) ceftriaxone treated group: received ceftriaxone at the dose 200 mg\kg/12 hrs via i.p. injection daily from the 6th dose of PTZ for 3 days. Racine score, latency before beginning the first myoclonic jerk and duration of the jerks used as parameters of behavioral assessment. Immunohistopathological study for Cx-43 expression in hippocampus and measurement of markers of oxidative stress (malondialdehyde [MDA], low reduced glutathione [GSH] and catalase [CAT]) in hippocampal neurons were done.
  
  
  RESULTS
  PTZ kindling was associated with behavioral changes (in the form high stage of Racine score, long seizure duration and short latency for the first jerk), enhanced oxidative stress state (as demonstrated by high MDA, low GSH and CAT) and up regulation of Cx43 in hippocampal regions. While, ceftriaxone treatment ameliorated, significantly, PTZ-induced convulsions and caused significant improvement in oxidative stress markers and Cx-43 expression in hippocamal regions (p < 0.05).
  
  
  CONCLUSIONS
  These findings support the anticonvulsive effects of some beta-lactams antibiotics which could offer a possible contributor in the basic treatment of temporal lobe epilepsy. This effect might be due to reduction of oxidative stress and Cx43 expression.
authors.affiliation: Department of Medical Physiology, Mansoura University, Mansoura, Egypt.
authors.forename: Abdelaziz M
authors.initials: AM
authors.lastname: Hussein
authors.affiliation: Department of Medical Physiology, Mansoura University, Mansoura, Egypt.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Ghalwash
authors.affiliation: Faculty of Medicine, Mansoura University, Mansoura, Egypt.
authors.forename: Khaled
authors.initials: K
authors.lastname: Magdy
authors.affiliation: Chemistry and Drug Metabolism, IRP, National Institute on Drug Abuse, National Institutes of Health, Biomedical Research Center, Baltimore, USA.
authors.forename: Osama A
authors.initials: OA
authors.lastname: Abulseoud
keywords: Ceftriaxone; Connexin 43; Epilepsy; Malondialdehyde (MDA); Pentylenetetrazole; Rats