title: FNDC3B promotes cell migration and tumor metastasis in hepatocellular carcinoma.
abstract: Recurrence and metastasis are common in hepatocellular carcinoma (HCC) and correlate with poor prognosis. We investigated the role of fibronectin type III domain containing 3B (FNDC3B) in HCC metastasis. Overexpression of FNDC3B in HCC cell lines enhanced cell migration and invasion. On the other hand, knockdown of FNDC3B using short-hairpin RNA reduced tumor nodule formation in both intra- and extra-hepatic metastasis. High levels of FNDC3B were observed in metastatic HCCs and correlated with poor patient survival and shorter recurrence time. Mutagenesis and LC-MS/MS analyses showed that FNDC3B promotes cell migration by cooperating with annexin A2 (ANXA2). Furthermore, FNDC3B and ANXA2 expression correlated negatively with patient survival. Our results indicate that FNDC3B behaves like an oncogene by promoting cell migration. This suggests FNDC3B could serve as a biomarker and therapeutic target for HCC metastasis.
authors.affiliation: VYM Genome Research Center, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Chin-Hui
authors.initials: CH
authors.lastname: Lin
authors.affiliation: VYM Genome Research Center, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Yao-Wen
authors.initials: YW
authors.lastname: Lin
authors.affiliation: VYM Genome Research Center, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Ying-Chun
authors.initials: YC
authors.lastname: Chen
authors.affiliation: Proteomics Research Center, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Chen-Chung
authors.initials: CC
authors.lastname: Liao
authors.affiliation: Institutes of Biomedical Sciences, Academia Sinica, Taipei, Taiwan.
authors.forename: Yuh-Shan
authors.initials: YS
authors.lastname: Jou
authors.affiliation: VYM Genome Research Center, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Ming-Ta
authors.initials: MT
authors.lastname: Hsu
authors.affiliation: VYM Genome Research Center, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Chian-Feng
authors.initials: CF
authors.lastname: Chen
mesh_terms: D000818:Animals; D017306:Annexin A2 / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000401:mortality; D045744:Cell Line, Tumor; D002465:Cell Movement; D002853:Chromatography, Liquid; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D053208:Kaplan-Meier Estimate; D008113:Liver Neoplasms / Q000378:metabolism* / Q000401:mortality; D051379:Mice; D008807:Mice, Inbred BALB C; D016296:Mutagenesis; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D020411:Oligonucleotide Array Sequence Analysis; D034741:RNA, Small Interfering / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: C472822:ANXA2 protein, human; D017306:Annexin A2; D014408:Biomarkers, Tumor; C543006:FNDC3B protein, human; D005353:Fibronectins; D034741:RNA, Small Interfering
keywords: FNDC3B; hepatocellular carcinoma; metastasis