title: Validation of an automated assay for the measurement of cupric reducing antioxidant capacity in serum of dogs.
abstract: BACKGROUND
  The objective of the present study was to optimize and validate an automated method to assess the total antioxidant capacity (TAC) in serum of dogs using the cupric reducing antioxidant capacity (CUPRAC) methodology (TACc) with bathocuproinedisulfonic acid disodium salt as chelating agent, evaluating also possible variations due to the use of two different automated analyzers. The method is based on the reduction of Cu(2+) into Cu(1+) by the action of the non-enzymatic antioxidants that are present in the sample.
  
  
  RESULTS
  Imprecision was low in both apparatus utilized, and the results were linear across serial Trolox and canine serum samples dilutions. Lipids did not interfere with the assay; however, hemolysis increased the TACc concentrations. When TACc concentrations were determined in ten healthy (control) dogs and in twelve dogs with inflammatory bowel disease (IBD), dogs with IBD had lower TACc concentrations when compared with the healthy dogs.
  
  
  CONCLUSIONS
  The method validated in this paper is precise, simple, and fast and can be easily adapted to automated analyzers.
authors.affiliation: Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU, Faculty of Veterinary Medicine, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Espinardo, Murcia, 30100, Spain.
authors.forename: Camila Peres
authors.initials: CP
authors.lastname: Rubio
authors.affiliation: Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU, Faculty of Veterinary Medicine, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Espinardo, Murcia, 30100, Spain.|Department of Animal Medicine and Surgery, Veterinary School, University Autonoma of Barcelona, Barcelona, Spain.
authors.forename: Asta
authors.initials: A
authors.lastname: Tvarijonaviciute
authors.affiliation: Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU, Faculty of Veterinary Medicine, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Espinardo, Murcia, 30100, Spain.
authors.forename: Silvia
authors.initials: S
authors.lastname: Martinez-Subiela
authors.affiliation: Department of Plant Biology (Plant Physiology), Faculty of Biology, University of Murcia, Murcia, Spain.
authors.forename: Josefa
authors.initials: J
authors.lastname: Hernández-Ruiz
authors.affiliation: Interdisciplinary Laboratory of Clinical Analysis Interlab-UMU, Faculty of Veterinary Medicine, Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Espinardo, Murcia, 30100, Spain. jjceron@um.es.
authors.forename: José Joaquin
authors.initials: JJ
authors.lastname: Cerón
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D001331:Automation; D001774:Blood Chemical Analysis / Q000295:instrumentation / Q000592:standards / Q000662:veterinary*; D003300:Copper / Q000378:metabolism; D004285:Dogs / Q000097:blood*; D010084:Oxidation-Reduction; D010618:Phenanthrolines / Q000378:metabolism; D015203:Reproducibility of Results
chemical_list: D000975:Antioxidants; D010618:Phenanthrolines; C028559:bathocuproine sulfonate; D003300:Copper
keywords: Bathocuproinedisulfonic acid; CUPRAC; Dogs; Oxidative stress; Validation