title: The effect of progesterone and 17-β estradiol on membrane-bound HLA-G in adipose derived stem cells.
abstract: Membrane-bound HLA-G (mHLA-G) discovery on adipose derived stem cells (ADSCs) as a tolerogenic and immunosuppressive molecule was very important. Many documents have shown that HLA-G expression can be controlled via some hormones such as progesterone (P4) and estradiol (E2). Therefore, this study was designed to evaluate progesterone and estradiol effects on mHLA-G in ADSCs at restricted and combination concentrations. Three independent cell lines were cultured in complete free phenol red DMEM and subcultured to achieve suffi cient cells. These cells were treated with P4, E2 and P4 plus E2 at physiologic and pregnancy concentrations for 3 days in cell culture conditions. The HLA-G positive ADSCs was measured via monoclonal anti HLA-G-FITC/MEMG-09 by means of flow cytometry in nine groups. Data were analyzed by one way ANOVA and Tukey's post hoc tests. There were no signifi cant values of the mean percentage of HLA-G positive cells in E2-treated and the combination of P4 plus E2-treated ADSCs compared to control cells (p value>0.05) but P4 had a signifi cant increase on mHLA-G in ADSCs (p value<0.05). High P4 concentration increased mHLA-G but E2 and the combination of P4 plus E2 could not change mHLA-G on ADSCs.
authors.affiliation: Department of Immunology, Medical School, Isfahan University of Medical Sciences, Isfahan 81746-73461, Iran.
authors.forename: Akram
authors.initials: A
authors.lastname: Moslehi
authors.affiliation: Department of Anatomical Science and Molecular Biology, Medical School, Isfahan University of Medical Sciences, Isfahan 81746-73461, Iran.
authors.forename: Batool
authors.initials: B
authors.lastname: Hashemi-Beni
authors.affiliation: Department of Physiology & Pharmacology, Medical School, Qom University of Medical Sciences, Qom 3713649373, Iran.
authors.forename: Azam
authors.initials: A
authors.lastname: Moslehi
authors.affiliation: Department of Anatomical Science and Molecular Biology, Medical School, Isfahan University of Medical Sciences, Isfahan 81746-73461, Iran.
authors.forename: Maryam Ali
authors.initials: MA
authors.lastname: Akbari
authors.affiliation: Department of Immunology, Medical School, Isfahan University of Medical Sciences, Isfahan 81746-73461, Iran.
authors.forename: Minoo
authors.initials: M
authors.lastname: Adib
keywords: Adipose derived stem cell; Estradiol; Membrane-bound HLA-G; Progesterone