title: miR-200b is a key regulator of tumor progression and metabolism targeting lactate dehydrogenase A in human malignant glioma.
abstract: Lactate dehydrogenase A (LDHA) is involved in various cancers. In this study, we investigated the expression and function of LDHA in glioma. We found that LDHA was up-regulated in glioma samples. Furthermore, we found that overexpression of LDHA promoted proliferation, invasion and glycolysis in glioma cells. Luciferase reporter assays confirmed that LDHA was a direct target of miR-200b. miR-200b was found to be down-regulated in glioma samples, which was inversely correlated with LDHA expression. Repression of LDHA by miR-200b suppressed the glycolysis, cell proliferation and invasion of glioma cells. These results provide evidence that miR-200b acts as a tumor suppressor in glioma through the inhibition of LDHA both in vitro and in vivo. Targeting LDHA through miR-200b could be a potential therapeutic strategy in glioma.
authors.affiliation: Department of Neurosurgery, Cancer Center of Guangzhou Medical University, Guangzhou, Guangdong, 510095, China.
authors.forename: Su
authors.initials: S
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Respiratory Disease, The 1st Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, 510120, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Jiang
authors.affiliation: Department of Neurosurgery, Cancer Center of Guangzhou Medical University, Guangzhou, Guangdong, 510095, China.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Luo
authors.affiliation: Department of Physiology, School of Basic Science, Guangzhou Medical University, Guangzhou, Guangdong, 510182, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Respiratory Disease, The 1st Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, 510120, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Fu
authors.affiliation: State Key Laboratory of Respiratory Disease, The 1st Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, 510120, China.
authors.forename: Yuqin
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Guangdong Provincial Hospital of TCM Breast Department, Guangzhou, Guangdong, 510006, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Song
authors.affiliation: Department of Neurosurgery, Cancer Center of Guangzhou Medical University, Guangzhou, Guangdong, 510095, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Cancer Center of Guangzhou Medical University, Guangzhou, Guangdong, 510095, China.
authors.forename: Hailin
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Neurosurgery, Cancer Center of Guangzhou Medical University, Guangzhou, Guangdong, 510095, China.
authors.forename: Yingfang
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Neurosurgery, Cancer Center of Guangzhou Medical University, Guangzhou, Guangdong, 510095, China.
authors.forename: Biao
authors.initials: B
authors.lastname: Peng
mesh_terms: D000328:Adult; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D018450:Disease Progression; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D005910:Glioma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D006019:Glycolysis; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D000080864:Lactate Dehydrogenase 5; D008156:Luciferases / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness / Q000235:genetics; D015854:Up-Regulation
chemical_list: D007527:Isoenzymes; C533896:MIRN200 microRNA, human; D035683:MicroRNAs; D007770:L-Lactate Dehydrogenase; D000080864:Lactate Dehydrogenase 5; D008156:Luciferases
keywords: LDHA; glioma; metabolism; miR-200b; proliferation