title: Foxp2 Regulates Identities and Projection Patterns of Thalamic Nuclei During Development.
abstract: The molecular mechanisms underlying the formation of the thalamus during development have been investigated intensively. Although transcription factors distinguishing the thalamic primordium from adjacent brain structures have been uncovered, those involved in patterning inside the thalamus are largely unclear. Here, we show that Foxp2, a member of the forkhead transcription factor family, regulates thalamic patterning during development. We found a graded expression pattern of Foxp2 in the thalamic primordium of the mouse embryo. The expression levels of Foxp2 were high in the posterior region and low in the anterior region of the thalamic primordium. In Foxp2 (R552H) knockin mice, which have a missense loss-of-function mutation in the forkhead domain of Foxp2, thalamic nuclei of the posterior region of the thalamus were shrunken, while those of the intermediate region were expanded. Consistently, Foxp2 (R552H) knockin mice showed changes in thalamocortical projection patterns. Our results uncovered important roles of Foxp2 in thalamic patterning and thalamocortical projections during development.
authors.affiliation: Department of Medical Neuroscience, Graduate School of Medical Sciences, Kanazawa University, Ishikawa 920-8640, Japan.|Brain/Liver Interface Medicine Research Center, Kanazawa University, Ishikawa 920-8640, Japan.|Department of Neurology, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.|Department of Molecular and Systems Neurobiology, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.
authors.forename: Haruka
authors.initials: H
authors.lastname: Ebisu
authors.affiliation: Department of Molecular and Systems Neurobiology, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.
authors.forename: Lena
authors.initials: L
authors.lastname: Iwai-Takekoshi
authors.affiliation: Department of Pediatrics, Jichi Medical University, Tochigi 329-0498, Japan.
authors.forename: Eriko
authors.initials: E
authors.lastname: Fujita-Jimbo
authors.affiliation: Department of Pathophysiology, Tokyo Medical University, Tokyo 160-8402, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Momoi
authors.affiliation: Department of Medical Neuroscience, Graduate School of Medical Sciences, Kanazawa University, Ishikawa 920-8640, Japan.|Brain/Liver Interface Medicine Research Center, Kanazawa University, Ishikawa 920-8640, Japan.|Department of Molecular and Systems Neurobiology, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kawasaki
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D019521:Body Patterning / Q000235:genetics*; D064032:Calbindin 2 / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism; D018274:Electroporation / Q000379:methods; D004622:Embryo, Mammalian; D018507:Gene Expression Regulation, Developmental / Q000235:genetics* / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D051542:Hepatocyte Nuclear Factor 3-beta / Q000235:genetics / Q000378:metabolism*; D060850:LIM-Homeodomain Proteins / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D008822:Mice, Transgenic; D009154:Mutation / Q000235:genetics*; D009434:Neural Pathways / Q000502:physiology*; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D036143:Receptor, EphA8 / Q000378:metabolism; D013787:Thalamic Nuclei / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D050599:Vesicular Glutamate Transport Protein 2 / Q000235:genetics / Q000378:metabolism; D000097573:Red Fluorescent Protein
chemical_list: D064032:Calbindin 2; D003851:Deoxyribonucleases; D049452:Green Fluorescent Proteins; D051542:Hepatocyte Nuclear Factor 3-beta; D060850:LIM-Homeodomain Proteins; D008164:Luminescent Proteins; D034741:RNA, Small Interfering; D036143:Receptor, EphA8; D014157:Transcription Factors; D050599:Vesicular Glutamate Transport Protein 2; D000097573:Red Fluorescent Protein; C496503:Foxa2 protein, mouse; C489784:Lhx2 protein, mouse; C493477:Slc17a6 protein, mouse; C485184:enhanced green fluorescent protein; C109663:caspase-activated deoxyribonuclease
keywords: Foxp2; thalamic patterning