title: Fibronectin-Grafted Titanium Dental Implants: An In Vivo Study.
abstract: Modification of the physiochemical properties of titanium surfaces using glow discharge plasma (GDP) and fibronectin coating has been shown to enhance the surface hydrophilicity, surface roughness, cell adhesion, migration, and proliferation. This in vivo study aimed to evaluate the bone integration efficacy of a biologically modified implant surface. Two different surface-modified implants (Ar-GDP and GDP-fib) were placed in the mandibular premolar area of six beagle dogs for 2-8 weeks. Three techniques [histologic evaluation, resonance frequency analysis (RFA), and microcomputed tomography (micro-CT) evaluation] were used to detect the implant stability and bone-implant contact. The implant stability quotient values of GDP-fib implants were significantly greater than the Ar-GDP implants at 2 and 4 weeks (P < 0.01). The bone volume/total volume ratio of GDP-fib implants was greater than the Ar-GDP implants in micro-CT evaluation. A high positive correlation was observed between RFA and micro-CT measurements. At 2 weeks, osteoblasts were seen to line the implant surface, and multinuclear osteoclasts could be seen on the surface of old parent bone. After 8 weeks, a majority of the space in the wound chamber appeared to be replaced by bone. Enhancement of the stability of biologically modified implants was proved by the results of RFA, micro-CT, and histological analysis. This enhanced stability may help fasten treatment and be clinically beneficial.
authors.affiliation: School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan.
authors.forename: Yu-Chi
authors.initials: YC
authors.lastname: Chang
authors.affiliation: School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan.
authors.forename: Kuo-Ning
authors.initials: KN
authors.lastname: Ho
authors.affiliation: School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan.
authors.forename: Sheng-Wei
authors.initials: SW
authors.lastname: Feng
authors.affiliation: School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan; Graduate Institute of Biomedical Materials & Tissue Engineering, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan.
authors.forename: Haw-Ming
authors.initials: HM
authors.lastname: Huang
authors.affiliation: Department of International Logistics, Chung-Ang University, Seoul 156756, Republic of Korea.
authors.forename: Chia-Hsun
authors.initials: CH
authors.lastname: Chang
authors.affiliation: School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan; Dental Department, Taipei Medical University Hospital, Taipei 110, Taiwan.
authors.forename: Che-Tong
authors.initials: CT
authors.lastname: Lin
authors.affiliation: School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan; Dental Department, Taipei Medical University Hospital, Taipei 110, Taiwan.
authors.forename: Nai-Chia
authors.initials: NC
authors.lastname: Teng
authors.affiliation: Department of General Dentistry, Chang Gung Memorial Hospital, Taipei 105, Taiwan; Graduate Institute of Dental & Craniofacial Science, Chang Gung University, Taoyuan 333, Taiwan.
authors.forename: Yu Hwa
authors.initials: YH
authors.lastname: Pan
authors.affiliation: School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan; Dental Department, Taipei Medical University, Shuang-Ho Hospital, Taipei 235, Taiwan.
authors.forename: Wei-Jen
authors.initials: WJ
authors.lastname: Chang
mesh_terms: D000818:Animals; D000069343:Bone-Implant Interface / Q000000981:diagnostic imaging*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D015921:Dental Implants; D004285:Dogs; D005353:Fibronectins / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*; D055114:X-Ray Microtomography
chemical_list: D020099:Coated Materials, Biocompatible; D015921:Dental Implants; D005353:Fibronectins; D014025:Titanium