title: Low dose effects of a Withania somnifera extract on altered marble burying behavior in stressed mice.
abstract: OBJECTIVE
  Withania somnifera root (WSR) extracts are often used in traditionally known Indian systems of medicine for prevention and cure of psychosomatic disorders. The reported experiment was designed to test whether low daily oral doses of such extracts are also effective in suppressing marble burying behavior in stressed mice or not.
  
  
  METHODS
  Groups of mice treated with 10, 20, or 40 mg/kg daily oral doses of WSR were subjected to a foot shock stress-induced hyperthermia test on the 1(st), 5(th), 7(th), and 10(th) day of the experiment. On the 11(th) and 12(th) treatment days, they were subjected to marble burying tests. Stress response suppressing effects of low dose WSR were estimated by its effects on body weight and basal core temperature of animals during the course of the experiment.
  
  
  RESULTS
  Alterations in bodyweight and basal core temperature triggered by repeated exposures to foot shock stress were absent even in the 10 mg/kg/day WSR treated group, whereas the effectiveness of the extract in foot shock stress-induced hyperthermia and marble burying tests increased with its increasing daily dose.
  
  
  CONCLUSIONS
  Marble burying test in stressed mice is well suited for identifying bioactive constituents of W. somnifera like medicinal plants with adaptogenic, anxiolytic and antidepressant activities, or for quantifying pharmacological interactions between them.
authors.affiliation: Department of Pharmaceutics, Neuropharmacology Research Laboratory, Indian Institute of Technology, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
authors.forename: Amitabha
authors.initials: A
authors.lastname: Dey
authors.affiliation: (Retired) Head of Pharmacology Research Laboratories, Dr. Willmar Schwabe GmbH & Co. KG, Karlsruhe, Germany.
authors.forename: Shyam Sunder
authors.initials: SS
authors.lastname: Chatterjee
authors.affiliation: Department of Pharmaceutics, Neuropharmacology Research Laboratory, Indian Institute of Technology, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
authors.forename: Vikas
authors.initials: V
authors.lastname: Kumar
keywords: Bioassay; Withania somnifera; foot shock stress; marble burying test; thermoregulation; treatment regimen