title: The Process of Separating Bovine Serum Albumin Using Hydroxyapatite and Active Babassu Coal (Orbignya martiana).
abstract: Bovine serum albumin is one of the major serum proteins; it plays an important role as a result of its functional and nutritional properties which have bioactive peptides. Adsorption method was used to separate protein, which involves hydroxyapatite, synthetic hydroxyapatite, and active babassu coal. Initially, characterization was carried out using the zeta potential of the adsorbents. Kinetic pseudo-first- and pseudo-second-order models were applied. For isotherms, equilibrium data studies were carried out using the Langmuir and Freundlich models, in addition to determining the efficiency of adsorptive process. The results of the zeta potential showed loads ranging from +6.9 to -42.8 mV. The kinetic data were better represented in the pseudo-second-order model with chemisorption characteristics. The adsorption capacity of the adsorbents decreased as pH increased, indicating that the electrostatic bonds and some functional groups of active babassu coal contributed to the reduction of adsorption, especially oxygen linked to carbon atoms. The value of pH 4.0 showed the best results of adsorption, being obtained as the maximum adsorption capacity (q m ) and yield (%) (where q m = 87.95 mg g(-1) and 74.2%; 68.26 mg g(-1) and 68.6%; and 36.18 mg g(-1), 37.4%) of hydroxyapatite, synthetic hydroxyapatite, and active babassu coal, respectively.
authors.affiliation: Biodiversity Network and Biotechnology Legal-Bionorte Amazon, Federal University of Tocantins, 109 Norte Avenida NS-15, ALCNO-14, Plan Director North, 77001-090 Palmas, TO, Brazil.
authors.forename: Márcia Regina
authors.identifier: 0000-0003-4801-5474
authors.initials: MR
authors.lastname: Ribeiro Alves
authors.affiliation: Biodiversity Network and Biotechnology Legal-Bionorte Amazon, Federal University of Tocantins, 109 Norte Avenida NS-15, ALCNO-14, Plan Director North, 77001-090 Palmas, TO, Brazil.
authors.forename: Abraham Damian Giraldo
authors.initials: AD
authors.lastname: Zuñiga
authors.affiliation: Department of Chemistry, Federal University of Viçosa, Avenida PH Holfs, 36570-000 Viçosa, MG, Brazil.
authors.forename: Rita de Cássia Superbi
authors.initials: Rde C
authors.lastname: Sousa
authors.affiliation: Department of Nutrition, Federal University of Espírito Santo, Avenida Fernando Ferrari, No. 514, Goiabeiras, 29075-010 Vitória, ES, Brazil.
authors.forename: Carmelita
authors.initials: C
authors.lastname: Zacchi Scolforo
mesh_terms: D000327:Adsorption; D028023:Arecaceae; D002606:Charcoal / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D012710:Serum Albumin, Bovine / Q000302:isolation & purification*; D014874:Water Pollutants, Chemical
chemical_list: D014874:Water Pollutants, Chemical; D002606:Charcoal; D012710:Serum Albumin, Bovine; D017886:Durapatite