title: Fibrin glue inhibits migration of ocular surface epithelial cells.
abstract: PurposeFibrin glue has been used successfully in numerous ophthalmic surgical procedures. Recently, fibrin glue has been used in limbal stem cell transplantation to reduce both operative time and to negate the need for sutures. The aim of this study was to determine the effects of fibrin glue on epithelial cell migration in vitro.MethodsCorneoscleral rims were split to retain the epithelial layer, Bowman's layer, and anterior stroma. Rims were cut into eight equal-sized pieces and were placed directly on culture plates or affixed with fibrin glue. Rims were maintained in culture for 25 days and epithelial cell growth was monitored. Cells were photographed to measure area or growth and immunofluorescence staining of explants for fibrin was performed.ResultsExplants that were glued demonstrated significantly delayed epithelial cell growth and migration as compared with explants without glue. By day 16, all fibrin glue had dissolved and coincided with onset of cell growth from glued explants. Cell growth commenced between days 3 and 4 for control explants without glue and around days 14-16 for explants with fibrin glue.ConclusionsFibrin glue delays epithelial cell migration by acting as a physical barrier and can potentially interfere with explant-derived limbal epithelial cell migration on to the corneal surface. We propose that glue should be used to attach the conjunctival frill of the limbal explant but care should be taken to ensure that the glue does not wrap around the explant if used to secure the explant as well. Strategic use of glue, to attach the recessed conjunctiva, can be advantageous in delaying conjunctival cell migration and reducing the need for sequential sector conjunctival epitheliectomy.
authors.affiliation: Larry A Donoso Laboratory for Eye Research, Section of Academic Ophthalmology, Division of Clinical Neuroscience, University of Nottingham, Nottingham, UK.
authors.forename: A M
authors.initials: AM
authors.lastname: Yeung
authors.affiliation: Larry A Donoso Laboratory for Eye Research, Section of Academic Ophthalmology, Division of Clinical Neuroscience, University of Nottingham, Nottingham, UK.
authors.forename: L A
authors.initials: LA
authors.lastname: Faraj
authors.affiliation: Larry A Donoso Laboratory for Eye Research, Section of Academic Ophthalmology, Division of Clinical Neuroscience, University of Nottingham, Nottingham, UK.
authors.forename: O D
authors.initials: OD
authors.lastname: McIntosh
authors.affiliation: Larry A Donoso Laboratory for Eye Research, Section of Academic Ophthalmology, Division of Clinical Neuroscience, University of Nottingham, Nottingham, UK.
authors.forename: V K
authors.initials: VK
authors.lastname: Dhillon
authors.affiliation: Larry A Donoso Laboratory for Eye Research, Section of Academic Ophthalmology, Division of Clinical Neuroscience, University of Nottingham, Nottingham, UK.
authors.forename: H S
authors.initials: HS
authors.lastname: Dua
mesh_terms: D002452:Cell Count; D002465:Cell Movement / Q000187:drug effects*; D017690:Cell Transplantation; D004847:Epithelial Cells / Q000166:cytology*; D019573:Epithelium, Corneal / Q000166:cytology*; D015718:Fibrin Tissue Adhesive / Q000494:pharmacology*; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D015948:Keratoplasty, Penetrating; D016850:Limbus Corneae / Q000166:cytology*; D009924:Organ Culture Techniques; D013234:Stem Cells / Q000166:cytology; D014014:Tissue Adhesives / Q000494:pharmacology*; D014019:Tissue Donors
chemical_list: D015718:Fibrin Tissue Adhesive; D014014:Tissue Adhesives