title: Heterogeneous Circulating Angiogenic Cell Responses to Acute Maximal Exercise.
abstract: Circulating angiogenic cells (CAC) comprise multiple subpopulations of exercise-inducible peripheral blood mononuclear cells (PBMC) that promote angiogenesis and maintain endothelial integrity. We examined the effect of acute maximal exercise on CD31, CD62E, CD14/CD31, CD34/VEGFR2, CD3/CD31, and CD3 PBMC in young, healthy adults.
  
  
  
  Blood samples were collected before and immediately after a graded treadmill exercise test for CAC analysis via flow cytometry.
  
  
  
  Maximal exercised produced 40%, 29%, 33%, 14%, and 33% increases in lymphocytic CD31, monolymphocytic CD31, CD62E, CD14/CD31, and CD34/VEGFR2 PBMC, respectively (P < 0.05). CD3/CD31 and CD3 cells were not altered with exercise. CD62E and CD14/CD31 PBMC were selectively augmented in women by 54% and 20%, respectively (P < 0.05). Exploratory analyses indicated that maximal exercise induced greater increases in CD62E and CD14/CD31 PBMC among women in the luteal phase compared with those in the follicular phase (P < 0.05). Basal lymphocytic PBMC and postexercise lymphocytic and monolymphocytic CD31 PBMC were lower among contraceptive users than nonusers.
  
  
  
  Maximal exercise induces a robust CAC response encompassing both progenitor and nonprogenitor cell types, with these effects differing between men and women for CD62E and CD14/CD31 cell types and the potential influence of menstrual cycle phase and contraceptive use.
authors.affiliation: Department of Kinesiology, University of Georgia, Athens, GA.
authors.forename: Daniel D
authors.initials: DD
authors.lastname: Shill
authors.forename: Meagan P
authors.initials: MP
authors.lastname: Marshburn
authors.forename: Hannah K
authors.initials: HK
authors.lastname: Hempel
authors.forename: Kasey A
authors.initials: KA
authors.lastname: Lansford
authors.forename: Nathan T
authors.initials: NT
authors.lastname: Jenkins
mesh_terms: D015703:Antigens, CD; D003278:Contraceptives, Oral, Hormonal / Q000008:administration & dosage; D004730:Endothelium, Vascular / Q000378:metabolism; D015444:Exercise / Q000502:physiology*; D005260:Female; D006801:Humans; D007434:Intrauterine Devices; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism*; D008297:Male; D008597:Menstrual Cycle / Q000502:physiology; D018919:Neovascularization, Physiologic; D012737:Sex Factors; D055815:Young Adult
chemical_list: D015703:Antigens, CD; D003278:Contraceptives, Oral, Hormonal