title: The Vibrio parahaemolyticus-infecting bacteriophage qdvp001: genome sequence and endolysin with a modular structure.
abstract: Vibrio parahaemolyticus, a marine pathogen, is a causative agent of gastroenteritis in humans after consumption of contaminated seafood. In recent years, infections with V. parahaemolyticus have become an increasingly frequent factor in microbial food poisoning; therefore, it is urgent to figure out ways to control Vibrio parahaemolyticus. Endolysins, lytic enzymes encoded by bacteriophages, have been regarded as a therapeutic alternative to antibiotics in control of bacterial growth and have been successfully utilized in various areas. Here, we report the full genome sequence of the novel phage qdvp001, which lyses Vibrio parahaemolyticus 17802. The qdvp001 genome consists of a 134,742-bp DNA with a G+C content of 35.35 % and 227 putative open reading frames. Analysis revealed that the qdvp001 open reading frames encoded various putative functional proteins with a putative endolysin gene (ORF 60). No holin genes were identified in qdvp001. ORF 60 was cloned and expressed. The results showed that the purified endolysin Lysqdvp001 had a high hydrolytic activity toward Vibrio parahaemolyticus and a broader spectrum compared to that of the parental bacteriophage qdvp001. Thus, purified endolysin Lysqdvp001 has a potential to be used as an antibacterial agent in the future.
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, People's Republic of China.
authors.forename: Weiyu
authors.initials: W
authors.lastname: Wang
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, People's Republic of China.
authors.forename: Mengzhe
authors.initials: M
authors.lastname: Li
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Lin
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, People's Republic of China. snow@ouc.edu.cn.
authors.forename: Jingxue
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, People's Republic of China.
authors.forename: Xiangzhao
authors.initials: X
authors.lastname: Mao
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000378:metabolism; D001433:Bacteriolysis; D001435:Bacteriophages / Q000201:enzymology* / Q000235:genetics*; D001482:Base Composition; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics*; D010450:Endopeptidases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D015870:Gene Expression; D023061:Gene Order; D016679:Genome, Viral; D006868:Hydrolysis; D016366:Open Reading Frames; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014736:Vibrio parahaemolyticus / Q000821:virology*
chemical_list: D000900:Anti-Bacterial Agents; D004279:DNA, Viral; D011994:Recombinant Proteins; D010450:Endopeptidases; C001143:endolysin