title: Isolation and characterization of Oya virus a member of Simbu serogroup, family Bunyaviridae, isolated from Karnataka, India.
abstract: During a study on Japanese encephalitis (JE) from Kolar district of Karnataka state, India in 1986; two virus isolates were obtained in infant Swiss albino mouse from a pig and a human serum sample. For characterization of these virus isolates, they were propagated in Vero CCL-81 cells. These virus isolates were screened for flaviviruses (Japanese encephalitis, West Nile, Dengue, Kyasanur forest disease) and Alphavirus (Chikungunya) by RT-PCR and found to be negative. Further these they were screened for bunyaviruses using genus-specific primers. A virus isolate from a human sample was sequenced using next generation sequencing; which identified it as Oya virus, Simbu group of the genus Orthobunyavirus of the family Bunyaviridae. Phylogenetic analysis of L, M, S (N and NSs) revealed its close association with Chinese strain of Oya virus in Simbu serogroup with the distance of 6.5>4.2>3.2% for nucleotides and 2.4>0.8>0.0% for the amino acid of L>M>S segments respectively. Based on the PCR results; an isolate from pig sample was also confirmed as Oya virus. This study was strengthened by findings of IgG antibody positivity against Oya virus in retrospective serum samples of suspected febrile illness cases from this area by an indigenously developed ELISA. Oya virus positivity was also recorded in human samples collected from Karnataka using nested RT-PCR. This is the first report of the presence of Oya virus in human samples. Further studies are needed to determine disease-causing potential in humans.
authors.affiliation: National Institute of Virology, Sus Road, Pashan, Pune 411 021, India.
authors.forename: Pragya
authors.initials: P
authors.lastname: Yadav
authors.affiliation: National Institute of Virology, Sus Road, Pashan, Pune 411 021, India.
authors.forename: Anita
authors.initials: A
authors.lastname: Shete
authors.affiliation: National Institute of Virology, Sus Road, Pashan, Pune 411 021, India.
authors.forename: Vijay
authors.initials: V
authors.lastname: Bondre
authors.affiliation: National Institute of Virology, Sus Road, Pashan, Pune 411 021, India.
authors.forename: Deepak
authors.initials: D
authors.lastname: Patil
authors.affiliation: National Institute of Virology, Sus Road, Pashan, Pune 411 021, India.
authors.forename: Prasad
authors.initials: P
authors.lastname: Kokate
authors.affiliation: National Institute of Virology, Sus Road, Pashan, Pune 411 021, India.
authors.forename: Sweena
authors.initials: S
authors.lastname: Chaudhari
authors.affiliation: National Institute of Virology, Sus Road, Pashan, Pune 411 021, India.
authors.forename: Sneha
authors.initials: S
authors.lastname: Srivastava
authors.affiliation: National Institute of Virology, Sus Road, Pashan, Pune 411 021, India.
authors.forename: Santosh
authors.initials: S
authors.lastname: Jadhav
authors.affiliation: National Institute of Virology, Sus Road, Pashan, Pune 411 021, India. Electronic address: directorniv@gmail.com.
authors.forename: Devendra
authors.initials: D
authors.lastname: Mourya
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002044:Bunyaviridae Infections / Q000821:virology*; D002522:Chlorocebus aethiops; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D007194:India; D065288:Serogroup; D012840:Simbu virus / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D013552:Swine; D014709:Vero Cells / Q000821:virology
chemical_list: D000914:Antibodies, Viral
keywords: IgG ELISA; India; Oya virus; PCR; Sequencing