title: Development and evaluation of PLGA polymer based nanoparticles of quercetin.
abstract: Quercetin is the most abundant antioxidant found in the human diet. Low aqueous solubility of quercetin limits its bioavailability and hence therapeutic effects. Therefore, the aim of the present study was to develop a poly lactide-co-glycolic acid (PLGA) polymer based nanoparticles of quercetin with a view to improve its aqueous solubility and examine the effect on its antioxidant and diuretic properties. Nanoparticles of quercetin were developed by single emulsion-solvent evaporation technique and evaluated in vitro for differential scanning calorimetry (DSC), Fourier transforms infra-red (FTIR) spectroscopy, particle size, polydispersity index and drug entrapment efficiency. Among the five different formulations (F1, F2, F3, F4 and F5), F2 and F3 were optimized with an average particle size of 189nm and 186nm and high entrapment values of 86.48%, 83.71%, respectively. SEM images of confirmed that prepared nanoparticles were spherical in shape with a smooth surface. In vitro release and anti-oxidant activity confirmed significant results. Furthermore, its in vivo diuretic activity was much better as compared to pure quercetin. The overall results suggest that PLGA polymer based nanoparticle could be a potential option for quercetin delivery.
authors.affiliation: Department of Pharmaceutics, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-kharj 11942, Saudi Arabia. Electronic address: mkanwer2002@yahoo.co.in.
authors.forename: Md Khalid
authors.initials: MK
authors.lastname: Anwer
authors.affiliation: Department of Pharmaceutics, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-kharj 11942, Saudi Arabia.
authors.forename: Mohammed A
authors.initials: MA
authors.lastname: Al-Mansoor
authors.affiliation: Department of Pharmaceutics, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-kharj 11942, Saudi Arabia.
authors.forename: Shahid
authors.initials: S
authors.lastname: Jamil
authors.affiliation: Department of Pharmaceutics, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-kharj 11942, Saudi Arabia.
authors.forename: Ramadan
authors.initials: R
authors.lastname: Al-Shdefat
authors.affiliation: Department of Pharmacology, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-kharj 11942, Saudi Arabia.
authors.forename: Mohammad Nazam
authors.initials: MN
authors.lastname: Ansari
authors.affiliation: Center of Excellence in Biotechnology Research (CEBR), King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia.
authors.forename: Faiyaz
authors.initials: F
authors.lastname: Shakeel
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000737:chemistry; D002152:Calorimetry, Differential Scanning; D002257:Carbonic Anhydrase Inhibitors / Q000494:pharmacology; D065546:Drug Liberation; D004573:Electrolytes / Q000737:chemistry; D019344:Lactic Acid / Q000737:chemistry*; D008297:Male; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D010851:Picrates / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011794:Quercetin / Q000008:administration & dosage / Q000494:pharmacology*; D017208:Rats, Wistar; D012015:Reference Standards; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D002257:Carbonic Anhydrase Inhibitors; D004573:Electrolytes; D010851:Picrates; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D011794:Quercetin; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Diuretic activity; PLGA nanoparticle; Quercetin