title: Effects of serostatus and gender on the HRV-16-induced local immune response.
abstract: The "experimental cold model" is widely used to investigate effects of HRV infection. However, effects of serostatus and gender on the HRV-induced immune response have not been clarified. 40 healthy seropositive and seronegative (1:1) male and female (1:1) subjects were inoculated with HRV-16. HRV infection increased viral load in nasal wash, which tended to be more pronounced in seronegative subjects. Furthermore, HRV infection increased levels of IP-10, IL-6, and IL-10 and leukocyte numbers in nasal wash of seronegative, but not of seropositive subjects. No differences in any of the parameters were found between both sexes. The HRV-induced local immune response is diminished in seropositive subjects compared with seronegative subjects, while gender does not influence this response. These results have important implications for the design of future experimental cold studies: seronegative subjects, from both sexes can be included.
authors.affiliation: Radboud University Medical Center, 6500 HB, Radboud Institute for Molecular Life Sciences, Department of Intensive Care Medicine, 6500 HB, Nijmegen, The Netherlands. Electronic address: rebecca.koch@radboudumc.nl.
authors.forename: Rebecca M
authors.initials: RM
authors.lastname: Koch
authors.affiliation: Radboud University Medical Center, 6500 HB, Radboud Institute for Molecular Life Sciences, Department of Intensive Care Medicine, 6500 HB, Nijmegen, The Netherlands.
authors.forename: Matthijs
authors.initials: M
authors.lastname: Kox
authors.affiliation: Radboud University Medical Center, 6500 HB, Radboud Institute for Molecular Life Sciences, Department of Intensive Care Medicine, 6500 HB, Nijmegen, The Netherlands.
authors.forename: Peter
authors.initials: P
authors.lastname: Pickkers
authors.affiliation: Radboud University Medical Center, 6500 HB, Radboud Institute for Molecular Life Sciences, Department of Pediatrics, 6500 HB, Nijmegen, The Netherlands.
authors.forename: Marien I
authors.initials: MI
authors.lastname: de Jonge
mesh_terms: D000914:Antibodies, Viral / Q000097:blood; D054357:Chemokine CXCL10 / Q000276:immunology; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000276:immunology; D015850:Interleukin-6 / Q000276:immunology; D007958:Leukocyte Count; D008297:Male; D010850:Picornaviridae Infections / Q000276:immunology*; D012229:Rhinovirus; D019562:Viral Load
chemical_list: D000914:Antibodies, Viral; C114753:CXCL10 protein, human; D054357:Chemokine CXCL10; C508609:IL10 protein, human; C508600:IL6 protein, human; D015850:Interleukin-6; D016753:Interleukin-10
keywords: Experimental cold; Gender; HRV-16; Serostatus