title: Larvicidal activity of Syzygium aromaticum (L.) Merr and Citrus sinensis (L.) Osbeck essential oils and their antagonistic effects with temephos in resistant populations of Aedes aegypti.
abstract: Environmentally friendly botanical larvicides are commonly considered as an alternative to synthetic larvicides against Aedes aegypti Linn. In addition, mosquito resistance to currently used larvicides has motivated research to find new compounds acting via different mechanisms of action, with the goal of controlling the spread of mosquitos. Essential oils have been widely studied for this purpose. This work aims to evaluate the larvicidal potential of Syzygium aromaticum and Citrus sinensis essential oils, either alone or in combination with temephos, on Ae. aegypti populations having different levels of organophosphate resistance. The 50% lethal concentration (LC50) of the essential oils alone and in combination with temephos and the influence of essential oils on vector oviposition were evaluated. The results revealed that essential oils exhibited similar larvicidal activity in resistant populations and susceptible populations. However, S. aromaticum and C. sinensis essential oils in combination with temephos did not decrease resistance profiles. The presence of the evaluated essential oils in oviposition sites significantly decreased the number of eggs compared to sites with tap water. Therefore, the evaluated essential oils are suitable for use in mosquito resistance management, whereas their combinations with temephos are not recommended. Additionally, repellency should be considered during formulation development to avoid mosquito deterrence.
authors.affiliation: Universidade Estadual Paulista, Faculdade de Ciências e Letras, Departamento de Ciências Biológicas, Assis, SP, Brasil.
authors.forename: Adriana Faraco de Oliveira
authors.initials: AF
authors.lastname: Araujo
authors.affiliation: Universidade Estadual Paulista, Faculdade de Ciências e Letras, Departamento de Ciências Biológicas, Assis, SP, Brasil.
authors.forename: João Tadeu
authors.initials: JT
authors.lastname: Ribeiro-Paes
authors.affiliation: Superintendência de Controle de Endemias, Marília, SP, Brasil.
authors.forename: Juliana Telles de
authors.initials: JT
authors.lastname: Deus
authors.affiliation: Universidade Federal de Sergipe, Departamento de Farmácia, São Cristóvão, SE, Brasil.
authors.forename: Sócrates Cabral de Holanda
authors.initials: SC
authors.lastname: Cavalcanti
authors.affiliation: Universidade Federal de Sergipe, Departamento de Farmácia, São Cristóvão, SE, Brasil.
authors.forename: Rogéria de Souza
authors.initials: Rde S
authors.lastname: Nunes
authors.affiliation: Universidade Federal de Sergipe, Departamento de Química, São Cristóvão, SE, Brasil.
authors.forename: Péricles Barreto
authors.initials: PB
authors.lastname: Alves
authors.affiliation: Superintendência de Controle de Endemias, Marília, SP, Brasil.
authors.forename: Maria de Lourdes da Graça
authors.initials: Mde L
authors.lastname: Macoris
mesh_terms: D000330:Aedes; D000818:Animals; D032084:Citrus sinensis / Q000737:chemistry*; D004338:Drug Combinations; D007305:Insecticide Resistance / Q000187:drug effects; D007306:Insecticides; D007814:Larva / Q000187:drug effects; D009032:Mosquito Control / Q000379:methods; D009822:Oils, Volatile; D010058:Oviposition / Q000187:drug effects; D015203:Reproducibility of Results; D027842:Syzygium / Q000737:chemistry*; D000002:Temefos; D013997:Time Factors
chemical_list: D004338:Drug Combinations; D007306:Insecticides; D009822:Oils, Volatile; D000002:Temefos