title: Effect of water-immersion restraint stress on tryptophan catabolism through the kynurenine pathway in rat tissues.
abstract: The aim of this study was to clarify the effect of water-immersion restraint stress (WIRS) on tryptophan (Trp) catabolism through the kynurenine (Kyn) pathway in rat tissues. The tissues of rats subjected to 6 h of WIRS (+WIRS) had increased tryptophan 2,3-dioxygenase (TDO) and indoleamine 2,3-dioxygenase (IDO) activities and increased TDO and IDO1 (one of two IDO isozymes in mammals) mRNA expression levels, with decreased Trp and increased Kyn contents in the liver. +WIRS rats had unchanged TDO and IDO activities in the kidney, decreased TDO activity and unchanged IDO activity in the brain, and unchanged IDO activity in the lung and spleen, with increased Kyn content in all of these tissues. Pretreatment of stressed rats with RU486, a glucocorticoid antagonist, attenuated the increased TOD activity, but not the increased IDO activity, with partial recoveries of the decreased Trp and increased Kyn contents in the liver. These results indicate that WIRS enhances hepatic Trp catabolism by inducing both IDO1 and TDO in rats.
authors.affiliation: Department of Chemistry, Fujita Health University School of Medicine, Toyoake, Aichi, 470-1192, Japan. yohta@fujita-hu.ac.jp.
authors.forename: Yoshiji
authors.initials: Y
authors.lastname: Ohta
authors.affiliation: Human Health Sciences, Graduate School of Medicine and Faculty of Medicine, Kyoto University, Kyoto, 666-8507, Japan.
authors.forename: Hisako
authors.initials: H
authors.lastname: Kubo
authors.affiliation: Department of Chemistry, Fujita Health University School of Medicine, Toyoake, Aichi, 470-1192, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Yashiro
authors.affiliation: Department of Clinical Biochemistry, Faculty of Medical Technology, Fujita Health University School of Health Sciences, Toyoake, Aichi, 470-1192, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Ohashi
authors.affiliation: Human Health Sciences, Graduate School of Medicine and Faculty of Medicine, Kyoto University, Kyoto, 666-8507, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Tsuzuki
authors.affiliation: Human Health Sciences, Graduate School of Medicine and Faculty of Medicine, Kyoto University, Kyoto, 666-8507, Japan.
authors.forename: Naoya
authors.initials: N
authors.lastname: Wada
authors.affiliation: Human Health Sciences, Graduate School of Medicine and Faculty of Medicine, Kyoto University, Kyoto, 666-8507, Japan.|Department of Disease Control and Prevention, Fujita Health University Graduate School of Health Sciences, Toyoake, Aichi, 470-1192, Japan.
authors.forename: Yasuko
authors.initials: Y
authors.lastname: Yamamoto
authors.affiliation: Human Health Sciences, Graduate School of Medicine and Faculty of Medicine, Kyoto University, Kyoto, 666-8507, Japan.|Department of Disease Control and Prevention, Fujita Health University Graduate School of Health Sciences, Toyoake, Aichi, 470-1192, Japan.
authors.forename: Kuniaki
authors.initials: K
authors.lastname: Saito
mesh_terms: D000818:Animals; D003681:Dehydration / Q000378:metabolism*; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D007737:Kynurenine / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014364:Tryptophan / Q000378:metabolism*; D014366:Tryptophan Oxygenase / Q000378:metabolism
chemical_list: D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D007737:Kynurenine; D014364:Tryptophan; D014366:Tryptophan Oxygenase
keywords: Glucocorticoid; Indoleamine 2,3-Dioxygenase 1; Interferon-γ; Liver tryptophan catabolism; Tryptophan 2,3-Dioxygenase; Water-immersion restraint stress (rats)