title: DNA hypomethylation of CD133 promoter is associated with recurrent glioma.
abstract: Gliomas are the most common type of brain tumor in the central nervous system of adults, and are highly aggressive, resistant to treatment, and prone to recurrence. Brain tumor stem cells (BTSCs) are implicated in tumor initiation and recurrence. Cluster of differentiation (CD)133 is currently the most widely used BTSC marker; however, its role in glioma development and progression is largely unknown. In this study, we evaluated CD133 expression in pairs of primary and recurrent human glioma specimens from 24 patients. We found that recurrent gliomas have aberrantly upregulated CD133 levels. To clarify the mechanism underlying this observation, we assessed CD133 promoter (P)2 methylation status by bisulfite sequencing and found that P2 hypomethylation was associated with the increase in CD133 expression and glioma recurrence. These results suggest that CD133 overexpression in BTSCs due to P2 hypomethylation underlies glioma recurrence, which may provide insight into the mechanism of glioma recurrence and provide a basis for novel therapies for glioma treatment.
authors.affiliation: Pediatrics Lab, Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, Jiangsu 226001, P.R. China.
authors.forename: Baolan
authors.initials: B
authors.lastname: Sun
authors.affiliation: Department of Neurosurgery, The First People's Hospital of Yancheng, Jiangsu 224001, P.R. China.
authors.forename: Zhengqiang
authors.initials: Z
authors.lastname: Wan
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Nantong University, Jiangsu 226001, P.R. China.
authors.forename: Jianhong
authors.initials: J
authors.lastname: Shen
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Nantong University, Jiangsu 226001, P.R. China.
authors.forename: Lanchun
authors.initials: L
authors.lastname: Ni
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Nantong University, Jiangsu 226001, P.R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.affiliation: Laboratory Medicine Center, Affiliated Hospital of Nantong University, Jiangsu 226001, P.R. China.
authors.forename: Ming
authors.initials: M
authors.lastname: Cui
authors.affiliation: Laboratory Medicine Center, Affiliated Hospital of Nantong University, Jiangsu 226001, P.R. China.
authors.forename: Hongbing
authors.initials: H
authors.lastname: Ni
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Nantong University, Jiangsu 226001, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Nantong University, Jiangsu 226001, P.R. China.
authors.forename: Jinlong
authors.initials: J
authors.lastname: Shi
mesh_terms: D000071916:AC133 Antigen / Q000235:genetics*; D014408:Biomarkers, Tumor / Q000235:genetics; D001932:Brain Neoplasms / Q000235:genetics*; D045744:Cell Line, Tumor; D019175:DNA Methylation / Q000235:genetics*; D005910:Glioma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D009364:Neoplasm Recurrence, Local / Q000235:genetics* / Q000473:pathology; D014411:Neoplastic Stem Cells / Q000473:pathology; D011401:Promoter Regions, Genetic / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics
chemical_list: D000071916:AC133 Antigen; D014408:Biomarkers, Tumor; C000606023:PROM1 protein, human