title: First genotyping of Cryptosporidium spp. in pre-weaned calves, broiler chickens and children in Syria by PCR-RFLP analysis.
abstract: In this study, PCR-RFLP was used for the first time in Syria for genotyping Cryptosporidium species of man, calves and chickens. The total of 391 fecal samples included 213 from children with diarrhea (<5years), 67 from pre-weaned calves with diarrhea and 111 from broiler chicken farms. All samples were collected and examined with acid fast stain to detect the positive samples. Subsequently a nested-PCR test was performed on 35 positive samples (17 from calves, 11 from chicken, and 7 from children) targeting SSU rRNA gene, and was followed by RFLP analysis using three restriction enzymes SspI, VspI and MboII. Results showed that C. parvum was the only identified species in children and calves, on the other hand C. baileyi was identified in broilers in addition to another species with unknown RFLP profile in comparison to those which have been described in chicken. Further studies using more genes are needed to sequence and detect subtypes of this parasite.
authors.affiliation: Department of Microbiology, Faculty of Veterinary Medicine, Hama University, Hama, Syria. Electronic address: morshid_k@yahoo.com.
authors.forename: Morshed
authors.initials: M
authors.lastname: Kassouha
authors.affiliation: Department of Animal Biology, Faculty of Sciences, Damascus University, Damascus, Syria.
authors.forename: Chadi
authors.initials: C
authors.lastname: Soukkarieh
authors.affiliation: Department of Microbiology, Faculty of Veterinary Medicine, Hama University, Hama, Syria.
authors.forename: Abdulkarim
authors.initials: A
authors.lastname: Alkhaled
mesh_terms: D000818:Animals; D002417:Cattle / Q000469:parasitology*; D002645:Chickens / Q000469:parasitology*; D002675:Child, Preschool; D003457:Cryptosporidiosis / Q000469:parasitology*; D003458:Cryptosporidium / Q000235:genetics* / Q000302:isolation & purification; D016054:DNA, Protozoan / Q000235:genetics; D005243:Feces / Q000469:parasitology; D005838:Genotype; D006801:Humans; D007223:Infant; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012337:RNA, Ribosomal, 18S / Q000235:genetics*; D013593:Syria
chemical_list: D016054:DNA, Protozoan; D012337:RNA, Ribosomal, 18S
keywords: Calves; Chicken; Children; Cryptosporidium; PCR-RFLP; Syria