title: Detection of Hydrogen Peroxide by DAB Staining inArabidopsisLeaves.
abstract: In this protocol, thein situdetection of hydrogen peroxide (one of several reactive oxygen species) is described in matureArabidopsisrosette leaves by staining with 3,3'-diaminobenzidine (DAB) using an adaptation of previous methods (Thordal-Christensenet al., 1997; Bindschedleret al., 2006; Daudiet al., 2012). DAB is oxidized by hydrogen peroxide in the presence of some haem-containing proteins, such as peroxidases, to generate a dark brown precipitate. This precipitate is exploited as a stain to detect the presence and distribution of hydrogen peroxide in plant cells. The protocol can be modified slightly to detect hydrogen peroxide in different types of plant tissue.
authors.affiliation: Department of Biological Sciences, Royal Holloway University of London, Egham, UK; Department of Plant Pathology, University of California, Davis, CA, USA.
authors.forename: Arsalan
authors.initials: A
authors.lastname: Daudi
authors.affiliation: Department of Biological Sciences, Royal Holloway University of London, Egham, UK.
authors.forename: Jose A
authors.initials: JA
authors.lastname: O'Brien