title: Lineage-Specific Effector Signatures of Invariant NKT Cells Are Shared amongst γδ T, Innate Lymphoid, and Th Cells.
abstract: Invariant NKT cells differentiate into three predominant effector lineages in the steady state. To understand these lineages, we sorted undifferentiated invariant NK T progenitor cells and each effector population and analyzed their transcriptional profiles by RNAseq. Bioinformatic comparisons were made to effector subsets among other lymphocytes, specifically Th cells, innate lymphoid cells (ILC), and γδ T cells. Myc-associated signature genes were enriched in NKT progenitors, like in other hematopoietic progenitors. Only NKT1 cells, but not NKT2 and NKT17 cells, had transcriptome similarity to NK cells and were also similar to other IFN-γ-producing lineages such as Th1, ILC1, and intraepithelial γδ T cells. NKT2 and NKT17 cells were similar to their analogous subsets of γδ T cells and ILCs, but surprisingly, not to Th2 and Th17 cells. We identified a set of genes common to each effector lineage regardless of Ag receptor specificity, suggesting the use of conserved regulatory cores for effector function.
authors.affiliation: Department of Laboratory Medicine and Pathology, Center for Immunology, University of Minnesota, Minneapolis, MN 55455; leey@umn.edu hogqu001@umn.edu.
authors.forename: You Jeong
authors.initials: YJ
authors.lastname: Lee
authors.affiliation: Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455; and.
authors.forename: Gabriel J
authors.identifier: 0000-0001-5871-5306
authors.initials: GJ
authors.lastname: Starrett
authors.affiliation: Department of Laboratory Medicine and Pathology, Center for Immunology, University of Minnesota, Minneapolis, MN 55455;
authors.forename: Seungeun Thera
authors.initials: ST
authors.lastname: Lee
authors.affiliation: Supercomputing Institute for Advanced Computational Research, University of Minnesota, Minneapolis, MN 55455.
authors.forename: Rendong
authors.initials: R
authors.lastname: Yang
authors.affiliation: Supercomputing Institute for Advanced Computational Research, University of Minnesota, Minneapolis, MN 55455.
authors.forename: Christine M
authors.identifier: 0000-0003-4566-2211
authors.initials: CM
authors.lastname: Henzler
authors.affiliation: Department of Laboratory Medicine and Pathology, Center for Immunology, University of Minnesota, Minneapolis, MN 55455;
authors.forename: Stephen C
authors.initials: SC
authors.lastname: Jameson
authors.affiliation: Department of Laboratory Medicine and Pathology, Center for Immunology, University of Minnesota, Minneapolis, MN 55455; leey@umn.edu hogqu001@umn.edu.
authors.forename: Kristin A
authors.initials: KA
authors.lastname: Hogquist
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000276:immunology; D019070:Cell Lineage / Q000235:genetics / Q000276:immunology*; D005434:Flow Cytometry; D007113:Immunity, Innate / Q000235:genetics / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D055611:Natural Killer T-Cells / Q000276:immunology*; D016133:Polymerase Chain Reaction; D016692:Receptors, Antigen, T-Cell, gamma-delta; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D059467:Transcriptome
chemical_list: D016692:Receptors, Antigen, T-Cell, gamma-delta