title: Role of growth media and chemical enhancers in secondary metabolites production from Aspergillus carbonarius (NRL-369) and their pharmaceutical potentials.
abstract: The present study investigates the effect of different growth media and chemical enhancer on silent genes in Aspergillus carbonarius (NRL-369) for secondary metabolites production and its in vitro biological activities. Results revealed that Aspergillus carbonarius (NRL-369) grown in Czapeak yeast extract broth medium produced more metabolites compared with other media. Chemical epigenetic modifiers (suberoyl-anilide hydroxamic acid (SAHA) and 5-azacytidine (5-AZA) at concentration of 15mM were effective for the expression of silent genes resulting in increased secondary metabolites production. Secondary metabolites extracted in ethyl acetate and fractionized in n-Hexane showed variable degree of growth inhibitions of the tested microorganisms. Similarly, these samples were also active against brine shrimps and Lemna.
authors.affiliation: Institute of Integrative Biosciences, CECOS University of Emerging Sciences, Hayatabad, Peshawar, KPK, Pakistan.
authors.forename: Abid Ali
authors.initials: AA
authors.lastname: Khan
authors.affiliation: Centre of Biotechnology and Microbiology, University of Peshawar, Pakistan.
authors.forename: Nafess
authors.initials: N
authors.lastname: Bacha
authors.affiliation: Centre of Biotechnology and Microbiology, University of Peshawar, Pakistan.
authors.forename: Bashir
authors.initials: B
authors.lastname: Ahmad
authors.affiliation: School of Chemistry, University of Bristol, Cantock's Close, Bristol BS8 1TS, UK.
authors.forename: R J
authors.initials: RJ
authors.lastname: Cox
authors.affiliation: Institute of Biotechnology and Genetic Engineering, The University of Agriculture Peshawar, KPK Pakistan.
authors.forename: Jehan
authors.initials: J
authors.lastname: Bakht
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000494:pharmacology; D001156:Artemia; D001230:Aspergillus / Q000187:drug effects / Q000378:metabolism*; D001374:Azacitidine / Q000494:pharmacology; D003470:Culture Media
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D000970:Antineoplastic Agents; D003470:Culture Media; D001374:Azacitidine