title: IgE-binding potencies of three peach Pru p 1 isoforms.
abstract: Pru p 1, the Bet v 1 homologue from peach, has been identified as a clinically relevant allergen. Three isoforms have been described, two in peach fruit (Pru p 1.0101 and Pru p 1.0201) and one in pollen (Pru p 1.0301). The present study aimed to compare their IgE-binding potencies.
  
  
  
  Three Pru p 1 isoforms were cloned and expressed as soluble proteins with His-tags in Escherichia coli. Protein identity was confirmed by MS, circular dichroism, and RNAse activity. IgE-binding capacity using ELISA and ImmunoCAP was compared. Three Pru p 1 isoforms had quite similar IgE-binding potencies for 60% of the sera, but more than twofold between any two isoforms among 40% of the 47 sera. The mean IgE binding of Pru p 1.0201 was slightly higher than other two isoforms. In a sera pool, homologous ImmunoCAP inhibition was higher than other two heterologous isoforms. Individual serum with diverse IgE values of three isoforms demonstrated the higher IgE inhibition of specific isoform with higher IgE value.
  
  
  
  A similar and variable pattern of IgE recognition was observed among three Pru p 1 isoforms. The two new isoforms can be used as more accurate diagnostic reagents.
authors.affiliation: Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.|Allergy Research Center, Zhejiang University, Hangzhou, China.
authors.forename: Zhong-Shan
authors.initials: ZS
authors.lastname: Gao
authors.affiliation: Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
authors.forename: Zhao-Wei
authors.initials: ZW
authors.lastname: Yang
authors.affiliation: Department of Experimental Immunology, Academic Medical Center-University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Serge A
authors.initials: SA
authors.lastname: Versteeg
authors.affiliation: Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Gao
authors.affiliation: Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China.
authors.forename: Wan-Yi
authors.initials: WY
authors.lastname: Fu
authors.affiliation: Department of Allergy, The Second Affiliated Hospital of Zhejiang University, School of Medicine, Hangzhou, China.
authors.forename: Hui-Ying
authors.initials: HY
authors.lastname: Wang
authors.affiliation: Department of Allergy, The First Affiliated Hospital of Zhejiang University, School of Medicine, Hangzhou, China.
authors.forename: Jian-Ying
authors.initials: JY
authors.lastname: Zhou
authors.affiliation: Department of Experimental Immunology, Academic Medical Center-University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Jaap H
authors.initials: JH
authors.lastname: Akkerdaas
authors.affiliation: Department of Experimental Immunology, Academic Medical Center-University of Amsterdam, Amsterdam, The Netherlands.|Department of Otorhinolaryngology, Academic Medical Center-University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Ronald
authors.initials: R
authors.lastname: van Ree
mesh_terms: D000485:Allergens / Q000737:chemistry; D052179:Antigens, Plant / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D005512:Food Hypersensitivity / Q000175:diagnosis; D006801:Humans; D007073:Immunoglobulin E / Q000187:drug effects / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry / Q000276:immunology*; D000068239:Prunus persica / Q000737:chemistry* / Q000276:immunology
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D007074:Immunoglobulin G; D010940:Plant Proteins; C403252:Pru p 1 allergen; C061312:Bet v 1 allergen, Betula; D007073:Immunoglobulin E
keywords: Component-resolved diagnosis; Cross-reactivity; Isoallergen; Peach allergy; Pru p 1