title: Dissection of quantitative traits by bulk segregant mapping in a protoploid yeast species.
abstract: Since more than a decade ago, Saccharomyces cerevisiae has been used as a model to dissect complex traits, revealing the genetic basis of a large number of traits in fine detail. However, to have a more global view of the genetic architecture of traits across species, the examination of the molecular basis of phenotypes within non-conventional species would undoubtedly be valuable. In this respect, the Saccharomycotina yeasts represent ideal and potential non-model organisms. Here we sought to assess the feasibility of genetic mapping by bulk segregant analysis in the protoploid Lachancea kluyveri (formerly S. kluyveri) yeast species, a distantly related species to S. cerevisiae For this purpose, we designed a fluorescent mating-type marker, compatible with any mating-competent strains representative of this species, to rapidly create a large population of haploid segregants (>10(5) cells). Quantitative trait loci can be mapped by selecting and sequencing an enriched pool of progeny with extreme phenotypic values. As a test bed, we applied this strategy and mapped the causal loci underlying halotolerance phenotypes in L. kluyveri Overall, this study demonstrates that bulk segregant mapping is a powerful way for investigating the genetic basis of natural variations in non-model yeast organisms and more precisely in L. kluyveri.
authors.affiliation: Department of Genetics, Genomics and Microbiology, University of Strasbourg - CNRS, UMR7156, 67000 Strasbourg, France.
authors.forename: Anastasie
authors.initials: A
authors.lastname: Sigwalt
authors.affiliation: Department of Genetics, Genomics and Microbiology, University of Strasbourg - CNRS, UMR7156, 67000 Strasbourg, France.
authors.forename: Claudia
authors.initials: C
authors.lastname: Caradec
authors.affiliation: Department of Genetics, Genomics and Microbiology, University of Strasbourg - CNRS, UMR7156, 67000 Strasbourg, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Brion
authors.affiliation: Department of Genetics, Genomics and Microbiology, University of Strasbourg - CNRS, UMR7156, 67000 Strasbourg, France.
authors.forename: Jing
authors.initials: J
authors.lastname: Hou
authors.affiliation: Department of Genetics, Genomics and Microbiology, University of Strasbourg - CNRS, UMR7156, 67000 Strasbourg, France.
authors.forename: Jacky
authors.initials: J
authors.lastname: de Montigny
authors.affiliation: Department of Genetics, Genomics and Microbiology, University of Strasbourg - CNRS, UMR7156, 67000 Strasbourg, France.
authors.forename: Paul
authors.initials: P
authors.lastname: Jung
authors.affiliation: Sorbonne Universités, UPMC Univ. Paris 06, CNRS, Institut de Biologie Paris-Seine, Laboratory of Computational and Quantitative Biology, F-75005 Paris, France.
authors.forename: Gilles
authors.initials: G
authors.lastname: Fischer
authors.affiliation: CRCM, CNRS - UMR7258, Inserm - U1068, Institut Paoli-Calmettes, Aix-Marseille Université, UM105, F-13009 Marseille, France.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Llorente
authors.affiliation: Department of Genetics, Genomics and Microbiology, University of Strasbourg - CNRS, UMR7156, 67000 Strasbourg, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Friedrich
authors.affiliation: Department of Genetics, Genomics and Microbiology, University of Strasbourg - CNRS, UMR7156, 67000 Strasbourg, France schacherer@unistra.fr.
authors.forename: Joseph
authors.initials: J
authors.lastname: Schacherer
mesh_terms: D002874:Chromosome Mapping / Q000379:methods*; D017930:Genes, Reporter; D040641:Quantitative Trait Loci; D004718:Saccharomycetales / Q000235:genetics*; D013194:Staining and Labeling
keywords: BSA-seq; Lachancea kluyveri; halotolerance; quantitative traits; trait mapping