title: Epigenetics changes caused by the fusion of human embryonic stem cell and ovarian cancer cells.
abstract: To observe the effect of gene expression and tumorigenicity in hybrid cells of human embryonic stem cells (hESCs) and ovarian cancer cells in vitro and in vivo using a mouse model, and to determine its feasibility in reprogramming tumour cells growth and apoptosis, for a potential exploration of the role of hESCs and tumour cells fusion in the management of ovarian cancer. Stable transgenic hESCs (H1) and ovarian cancer cell line OVCAR-3 were established before fusion, and cell fusion system was established to analyse the related indicators. PTEN expression in HO-H1 cells was higher than those in the parental stem cells and lower than those in parental tumour cells; the growth of OV-H1 (RFP+GFP) hybrid cells with double fluorescence expressions were obviously slower than that of human embryonic stem cells and OVCAR-3 ovarian cancer cells. The apoptosis signal of the OV-H1 hybrid cells was significantly higher than that of the hESCs and OVCAR-3 ovarian cancer cells. In vivo results showed that compared with 7 days, 28 days and 35 days after inoculation of OV-H1 hybrid cells; also, apoptotic cell detection indicated that much stronger apoptotic signal was found in OV-H1 hybrid cells inoculated mouse. The hESCs can inhibit the growth of OVCAR-3 cells in vitro by suppressing p53 and PTEN expression to suppress the growth of tumour that may be achieved by inducing apoptosis of OVCAR-3 cells. The change of epigenetics after fusion of ovarian cancer cells and hESCs may become a novel direction for treatment of ovarian cancer.
authors.affiliation: Department of Obstetrics and Gynecology, the First Affiliated Hospital of Sun Yat-Sen University, GuangZhou 510080, China.
authors.forename: Ke
authors.initials: K
authors.lastname: He
authors.affiliation: Department of Urology, the Sixth Affiliated Hospital of Sun Yat-Sen University, GuangZhou 510655, China.
authors.forename: Hu
authors.initials: H
authors.lastname: Qu
authors.affiliation: Department of Urology, the Sixth Affiliated Hospital of Sun Yat-Sen University, GuangZhou 510655, China Reproductive Medicine Research Center, the Sixth Affiliated Hospital of Sun Yat-Sen University, GuangZhou 510655, China.
authors.forename: Li-Nan
authors.initials: LN
authors.lastname: Xu
authors.affiliation: Reproductive Medical Center, the First Affiliated Hospital of Sun Yat-Sen University, GuangZhou 510080, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Gao
authors.affiliation: Center for Stem Cell Biology and Tissue Engineering, Sun Yat-Sen University, Guangzhou 510080, China.
authors.forename: Fu-Yi
authors.initials: FY
authors.lastname: Cheng
authors.affiliation: Center for Stem Cell Biology and Tissue Engineering, Sun Yat-Sen University, Guangzhou 510080, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Xiang
authors.affiliation: Reproductive Medical Center, the First Affiliated Hospital of Sun Yat-Sen University, GuangZhou 510080, China zcqsys1h@outlook.com.
authors.forename: Can-Quan
authors.initials: CQ
authors.lastname: Zhou
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D002459:Cell Fusion; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D019008:Drug Resistance, Neoplasm; D044127:Epigenesis, Genetic; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D000066449:Human Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D006822:Hybrid Cells / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D010051:Ovarian Neoplasms / Q000378:metabolism / Q000473:pathology*; D051059:PTEN Phosphohydrolase / Q000235:genetics; D023041:Xenograft Model Antitumor Assays
chemical_list: D051059:PTEN Phosphohydrolase
keywords: PTEN; epigenetics; fusion; human embryonic stem cell; ovarian cancer cell; p53