title: Immunohistochemical Expression and Clinical Significance of Wnt11 and BCL2A1 in Complete Moles.
abstract: OBJECTIVE
  To investigate Wnt11 and BCL2A1 immunohistochemical expression in complete moles and normal villi.
  
  
  METHODS
  The expression of Wnt11 and BCL2A1 in 84 complete moles and 30 normal first-trimester villi were detected by Envision immunohistochemistry. Quantitative evaluation according to color deconvolution and immunoreactive score was performed. Data was analyzed using Kruskal-Wallis test, Pearson test, and ROC curve.
  
  
  RESULTS
  Of 84 complete moles, 14 developed to post-molar gestational trophoblastic neoplasia, and the others regressed spontaneously. Both proteins showed cytoplasmic pattern, whereas the DAB wt% of BCL2A1 and Wnt11 expression was highest in moles that developed to GTN, gradually reduced in spontaneously regressed moles and normal villi (all p < 0.01). We considered a 23.17% cutoff valuefor Wnt11 DAB wt% and 16.31% for BCL2A1 DAB wt% to assess molar progression to GTN. There was positive correlation between expressions of the 2 proteins (r = 0.403).
  
  
  CONCLUSIONS
  Our findings demonstrated immunohistochemical expression of Wnt11 and BCL2A1 in complete moles and normal villi. Both proteins may be included as part of an immunohistochemical panel to identify postmolar outcome when other trophoblastic markers yield ambiguous results.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Hao
authors.forename: Weiguo
authors.initials: W
authors.lastname: Lü
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Cheng
authors.forename: Caiyun
authors.initials: C
authors.lastname: Zhou
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000032:analysis*; D016022:Case-Control Studies; D005260:Female; D005865:Gestational Age; D006801:Humans; D006828:Hydatidiform Mole / Q000737:chemistry* / Q000473:pathology; D007090:Image Interpretation, Computer-Assisted; D007150:Immunohistochemistry; D015778:Minor Histocompatibility Antigens; D011237:Predictive Value of Tests; D011247:Pregnancy; D011379:Prognosis; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis*; D012372:ROC Curve; D015203:Reproducibility of Results; D014594:Uterine Neoplasms / Q000737:chemistry* / Q000473:pathology; D051153:Wnt Proteins / Q000032:analysis*; D055815:Young Adult
chemical_list: C096816:BCL2-related protein A1; D014408:Biomarkers, Tumor; D015778:Minor Histocompatibility Antigens; D019253:Proto-Oncogene Proteins c-bcl-2; D051153:Wnt Proteins; C540523:Wnt11 protein, human