title: Utilizing the micron sized non-thermal atmospheric pressure plasma inside the animal body for the tumor treatment application.
abstract: This study aimed to evaluate the effects of micron sized non-thermal atmospheric pressure plasma inside the animal body on breast cancer tumor. The μ-plasma jet consists of micron sized hollow tube in which pure helium gas is ionized by high voltage (4 kV) and high frequency (6 kHz). The efficiency of the plasma treatment in killing cancer cells was first investigated by cell viability measurements of treated 4T1 cells using flow cytometry and cell cycle analysis. For exploration of the in vivo effects of the plasma treatment, the BALB/c mice inoculated by 4T1 cell lines were exposed subcutaneously to plasma for 3 minutes. In addition, H&E staining, TUNEL and Western blotting assays were performed in order to observed the effects of the non-thermal plasma on the tumor cells. The results showed that the efficiency of the plasma in suppression of the tumor growth is comparable to that of a typical chemotherapy drug. Moreover, the results indicated that the plasma induces apoptosis in the tumor tissue and increases the ratio of the apoptotic to anti-apoptotic protein expression. We believe that these findings presented herein may extend our knowledge of the mechanisms by which the plasma exerts its promising anti-cancer effects.
authors.affiliation: Laser and Plasma institute, Shahid Beheshti University, Tehran, Iran.|Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Shahriar
authors.initials: S
authors.lastname: Mirpour
authors.affiliation: Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Somayeh
authors.initials: S
authors.lastname: Piroozmand
authors.affiliation: Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran.
authors.forename: Neda
authors.initials: N
authors.lastname: Soleimani
authors.affiliation: Plasma Physics Research Center, Science and Research branch of Islamic Azad University, Tehran, Iran.
authors.forename: Neda
authors.initials: N
authors.lastname: Jalali Faharani
authors.affiliation: Laser and Plasma institute, Shahid Beheshti University, Tehran, Iran.
authors.forename: Hamidreza
authors.initials: H
authors.lastname: Ghomi
authors.affiliation: Razi Drug Research Center and Department of Pharmacology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Hoda
authors.initials: H
authors.lastname: Fotovat Eskandari
authors.affiliation: Razi Drug Research Center and Department of Pharmacology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Ali Mohammad
authors.initials: AM
authors.lastname: Sharifi
authors.affiliation: Russell H. Morgan Department of Radiology and Radiologic Science, Johns Hopkins University, MD, USA.
authors.forename: Sahar
authors.initials: S
authors.lastname: Mirpour
authors.affiliation: Laser and Plasma institute, Shahid Beheshti University, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Eftekhari
authors.affiliation: Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Nikkhah
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D051017:Apoptosis Regulatory Proteins / Q000235:genetics*; D001274:Atmospheric Pressure; D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D045744:Cell Line, Tumor; D002470:Cell Survival; D004195:Disease Models, Animal; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006371:Helium / Q000737:chemistry / Q000627:therapeutic use*; D006801:Humans; D020287:In Situ Nick-End Labeling; D051379:Mice; D058626:Plasma Gases / Q000737:chemistry / Q000627:therapeutic use*
chemical_list: D051017:Apoptosis Regulatory Proteins; D058626:Plasma Gases; D006371:Helium