title: Effect of high hydrostatic pressure treatment on isoquercetin production from rutin by commercial α-L-rhamnosidase.
abstract: OBJECTIVE
  To optimize conversion of rutin to isoquercetin by commercial α-L-rhamnosidase using high hydrostatic pressure (HHP).
  
  
  RESULTS
  The de-rhamnosylation activity of α-L-rhamnosidase for isoquercetin production was maximal at pH 6.0 and 50 °C using HHP (150 MPa). The enzyme showed high specificity for rutin. The specific activity for rutin at HHP was 1.5-fold higher than that at atmospheric pressure. The enzyme completely hydrolysed 20 mM rutin in tartary buckwheat extract after 2 h at HHP, with a productivity of 10 mM h(-1). The productivity and conversion were 2.2- and 1.5-fold higher at HHP than at atmospheric pressure, respectively.
  
  
  CONCLUSIONS
  This is the first report concerning the enzymatic hydrolysis of isoquercetin in tartary buckwheat at HHP.
authors.affiliation: International Ginseng & Herb Research Institute, Geumsan, Republic of Korea.
authors.forename: Do-Yeon
authors.initials: DY
authors.lastname: Kim
authors.affiliation: Synthetic Biology & Bioengineering Research Center, KRIBB, Daejeon, Yuseong-gu, Republic of Korea.
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Yeom
authors.affiliation: Department of Food Science and Technology, Catholic University of Daegu, Hayang, Republic of Korea.
authors.forename: Chang-Su
authors.initials: CS
authors.lastname: Park
authors.affiliation: Freshwater Bioresources Utilization Division, Nakdonggang National Institute of Biological Resources, Sangju, Republic of Korea. yskim@nnibr.re.kr.
authors.forename: Yeong-Su
authors.identifier: http://orcid.org/0000-0002-9831-2883
authors.initials: YS
authors.lastname: Kim
mesh_terms: D002851:Chromatography, High Pressure Liquid; D019612:Fagopyrum / Q000737:chemistry*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D006874:Hydrostatic Pressure; D011794:Quercetin / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D012431:Rutin / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry
chemical_list: C016527:isoquercitrin; D012431:Rutin; D011794:Quercetin; D006026:Glycoside Hydrolases; C022525:alpha-L-rhamnosidase
keywords: Hydrostatic pressure; Isoquercetin; Rutin; Tartary buckwheat; α-L-rhamnosidase