title: Antibiotic and metal resistance in a ST395 Pseudomonas aeruginosa environmental isolate: A genomics approach.
abstract: We analyzed the resistome of Pseudomonas aeruginosa E67, an epiphytic isolate from a metal-contaminated estuary. The aim was to identify genetic determinants of resistance to antibiotics and metals, assessing possible co-selection mechanisms. Identification was based on phylogenetic analysis and average nucleotide identity value calculation. MLST affiliated E67 to ST395, previously described as a high-risk clone. Genome analysis allowed identifying genes probably involved in resistance to antibiotics (e.g. beta-lactams, aminoglycosides and chloramphenicol) and metals (e.g. mercury and copper), consistent with resistance phenotypes. Several genes associated with efflux systems, as well as genetic determinants contributing to gene motility, were identified. Pseudomonas aeruginosa E67 possesses an arsenal of resistance determinants, probably contributing to adaptation to a polluted ecosystem. Association to mobile structures highlights the role of these platforms in multi-drug resistance. Physical links between metal and antibiotic resistance genes were not identified, suggesting a predominance of cross-resistance associated with multidrug efflux pumps.
authors.affiliation: Biology Department, CESAM & IBIMED, University of Aveiro, Aveiro, Portugal.
authors.forename: Pedro
authors.initials: P
authors.lastname: Teixeira
authors.affiliation: Biology Department, CESAM & IBIMED, University of Aveiro, Aveiro, Portugal. Electronic address: martat@ua.pt.
authors.forename: Marta
authors.initials: M
authors.lastname: Tacão
authors.affiliation: Biology Department, CESAM, University of Aveiro, Aveiro, Portugal.
authors.forename: Artur
authors.initials: A
authors.lastname: Alves
authors.affiliation: Biology Department, CESAM & IBIMED, University of Aveiro, Aveiro, Portugal.
authors.forename: Isabel
authors.initials: I
authors.lastname: Henriques
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015373:Bacterial Typing Techniques; D005798:Genes, Bacterial; D023281:Genomics; D019216:Metals, Heavy / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D058885:Multilocus Sequence Typing; D010802:Phylogeny; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D019216:Metals, Heavy
keywords: Co-selection; Environment; Genome sequencing; Opportunistic pathogen; Pseudomonas aeruginosa; Resistome