title: MiRNA-200a expression is inverse correlation with hepatocyte growth factor expression in stromal fibroblasts and its high expression predicts a good prognosis in patients with non-small cell lung cancer.
abstract: Cancer-associated fibroblasts (CAFs) play an important role in favoring tumor progression. However, little is known concerning expression of miRNA-200a and its potential target gene hepatocyte growth factor (HGF) in CAFs. In the present study, we investigated expression levels and prognostic significance of miRNA-200a and HGF in stromal fibroblasts of non-small cell lung cancer (NSCLC), and evaluated the correlation between miRNA-200a and HGF. In situ hybridization and immunohistochemical staining were used to investigate expression levels of miRNA-200a and HGF in 134 formalin-fixed paraffin-embedded tumor specimens from clinical stage I -IIIA NSCLC, respectively. The results showed a significant inverse correlation existed between miRNA-200a and HGF expression level in stromal fibroblasts (χ2 = 21.778, p = 0.000). In vitro, the upregulation of miRNA-200a reduced expression of HGF protein in human CAFs. The 3-year overall survival (OS) rates with low and high miRNA-200a expression in stromal fibroblasts were 39.0% and 53.4%, respectively (χ2=4.25, p=0.039). The 3-year OS rates with low and high HGF expression in stromal fibroblasts were 60.3% and 31.8%, respectively (χ2=12.55, p=0.000). The multivariate analysis showed that clinical stage and HGF expression level in stromal fibroblasts were the independent predictive factors of OS. These results suggested that miRNA-200a expression was inverse correlation with HGF expression in stromal fibroblasts. High miRNA-200a and low HGF expression in stromal fibroblasts may predict a good prognosis in patients with NSCLC.
authors.affiliation: Department of Thoracic Surgery, the Second Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: Yongbing
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Oncology & Radiotherapy, the Second Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: Menghua
authors.initials: M
authors.lastname: Du
authors.affiliation: Department of Oncology & Radiotherapy, the Second Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Oncology & Radiotherapy, the Second Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Xing
authors.affiliation: Department of Pathology, the Second Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Pathology, the Second Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Oncology & Radiotherapy, the Second Affiliated Hospital of Soochow University, Suzhou, China.|Department of Radiation Oncology, Fudan University Shanghai Cancer Center, Shanghai, China.
authors.forename: Xueguan
authors.initials: X
authors.lastname: Lu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D000072645:Cancer-Associated Fibroblasts / Q000473:pathology*; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000401:mortality* / Q000473:pathology / Q000601:surgery; D005260:Female; D005500:Follow-Up Studies; D015972:Gene Expression Regulation, Neoplastic; D017228:Hepatocyte Growth Factor / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000235:genetics* / Q000401:mortality* / Q000473:pathology / Q000601:surgery; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D015996:Survival Rate
chemical_list: D014408:Biomarkers, Tumor; C066855:HGF protein, human; C533896:MIRN200 microRNA, human; D035683:MicroRNAs; D017228:Hepatocyte Growth Factor
keywords: hepatocyte growth factor; lung cancer; miRNA-200a; prognosis; stromal fibroblasts