title: The CtsR regulator controls the expression of clpC, clpE and clpP and is required for the virulence of Enterococcus faecalis in an invertebrate model.
abstract: The intrinsic ruggedness of Enterococcus faecalis is responsible for its widespread distribution in nature and is often viewed as an important virulence determinant. Previously, we showed that the ClpB ATPase is negatively regulated by CtsR and is required for thermotolerance and virulence in a Galleria mellonella invertebrate model. Here, we used in silico, Northern blot and quantitative real-time PCR analyses to identify additional members of the CtsR regulon, namely the clpP peptidase and the clpC and clpE ATPases. When compared to the parent strain, virulence of the ΔctsR strain in G. mellonella was significantly attenuated.
authors.affiliation: Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Avenida Carlos Chagas Filho, 373, Bloco I, Rio De Janeiro, RJ, 21941-902, Brazil.
authors.forename: Ana Paula Vaz
authors.initials: AP
authors.lastname: Cassenego
authors.affiliation: Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Avenida Carlos Chagas Filho, 373, Bloco I, Rio De Janeiro, RJ, 21941-902, Brazil.
authors.forename: Naira Elane Moreira
authors.initials: NE
authors.lastname: de Oliveira
authors.affiliation: Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Avenida Carlos Chagas Filho, 373, Bloco I, Rio De Janeiro, RJ, 21941-902, Brazil.
authors.forename: Marinella Silva
authors.initials: MS
authors.lastname: Laport
authors.affiliation: Department of Oral Biology, University of Florida College of Dentistry, Gainesville, FL, 32610, USA.
authors.forename: Jaqueline
authors.initials: J
authors.lastname: Abranches
authors.affiliation: Department of Oral Biology, University of Florida College of Dentistry, Gainesville, FL, 32610, USA.
authors.forename: José A
authors.initials: JA
authors.lastname: Lemos
authors.affiliation: Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Avenida Carlos Chagas Filho, 373, Bloco I, Rio De Janeiro, RJ, 21941-902, Brazil. marciagm@micro.ufrj.br.
authors.forename: Marcia
authors.initials: M
authors.lastname: Giambiagi-deMarval
mesh_terms: D000251:Adenosine Triphosphatases / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D049071:Endopeptidase Clp / Q000096:biosynthesis / Q000378:metabolism; D013293:Enterococcus faecalis / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity*; D015964:Gene Expression Regulation, Bacterial; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D018869:Heat-Shock Response / Q000235:genetics; D007915:Lepidoptera / Q000382:microbiology*; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D014774:Virulence; D037521:Virulence Factors / Q000096:biosynthesis / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; C087481:ClpC protein, Bacteria; C118338:CtsR protein, bacteria; D006360:Heat-Shock Proteins; D012097:Repressor Proteins; D037521:Virulence Factors; D049071:Endopeptidase Clp; D000251:Adenosine Triphosphatases; C118340:ClpE protein, bacteria
keywords: Enterococcus faecalis; Thermotolerance; Virulence; clp genes; ctsR regulon