title: Improvement of Peroxyl Radical Scavenging Bioassay Based on Bactericidal Action of tert-Butyl Peroxyl Radical.
abstract: An improved bioassay for measurement of peroxyl radical scavenging activity(1,2)) is described. The modifications included the use of (1) Enterococcus faecium strain S, (2) 25% dimethyl sulfoxide, (3) L-ascorbic acid for the termination of the bactericidal reaction, and (4) the concentration of antioxidants that gives 75% of the log (colony forming unit/ml) of the control reaction (without methemoglobin or tert-butyl hydroperoxide), for the expression of antioxidant activity.
authors.affiliation: a Department of Food and Nutrition, Faculty of Home Economics, Japan Women's University.
authors.forename: T
authors.initials: T
authors.lastname: Kawasumi
authors.affiliation: b OTC Drug R&D Department, Wakamoto Pharmaceutical Co., Ltd.
authors.forename: M
authors.initials: M
authors.lastname: Sato
authors.affiliation: b OTC Drug R&D Department, Wakamoto Pharmaceutical Co., Ltd.
authors.forename: Y
authors.initials: Y
authors.lastname: Tsuriya
authors.affiliation: b OTC Drug R&D Department, Wakamoto Pharmaceutical Co., Ltd.
authors.forename: S
authors.initials: S
authors.lastname: Ueno
keywords: antioxidant; bioassay; synergism; t-butyl peroxyl radical scavenging