title: Salt Stress Reveals a New Role for ARGONAUTE1 in miRNA Biogenesis at the Transcriptional and Posttranscriptional Levels.
abstract: Plants as sessile organisms have developed prompt response mechanisms to react to rapid environmental changes. In addition to the transcriptional regulation of gene expression, microRNAs (miRNAs) are key posttranscriptional regulators of the plant stress response. We show here that the expression levels of many miRNAs were regulated under salt stress conditions. This regulation occurred at the transcriptional and posttranscriptional levels. During salinity stress, the levels of miRNA161 and miRNA173 increased, while the expression of pri-miRNA161 and pri-miRNA173 was down-regulated. Under salt stress conditions, miRNA161 and miRNA173 were stabilized in the cytoplasm, and the expressions of MIR161 and MIR173 were negatively regulated in the nucleus. ARGONAUTE1 (AGO1) participated in both processes. We demonstrated that AGO1 cotranscriptionally controlled the expression of MIR161 and MIR173 in the nucleus. Our results suggests that AGO1 interacts with chromatin at MIR161 and MIR173 loci and causes the disassembly of the transcriptional complex, releasing short and unpolyadenylated transcripts.
authors.affiliation: Department of Gene Expression, Faculty of Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, 61-614 Poznan, Poland (J.D., M.B., D.B., H.P., W.W., Z.S.-K., A.J.); andDepartment of Cell Biology, Faculty of Biology and Environment Protection, Nicolaus Copernicus University, 87-100 Torun, Poland (K.N., J.N.).
authors.forename: Jakub
authors.identifier: 0000-0001-6141-8863
authors.initials: J
authors.lastname: Dolata
authors.affiliation: Department of Gene Expression, Faculty of Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, 61-614 Poznan, Poland (J.D., M.B., D.B., H.P., W.W., Z.S.-K., A.J.); andDepartment of Cell Biology, Faculty of Biology and Environment Protection, Nicolaus Copernicus University, 87-100 Torun, Poland (K.N., J.N.).
authors.forename: Mateusz
authors.identifier: 0000-0001-7043-2827
authors.initials: M
authors.lastname: Bajczyk
authors.affiliation: Department of Gene Expression, Faculty of Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, 61-614 Poznan, Poland (J.D., M.B., D.B., H.P., W.W., Z.S.-K., A.J.); andDepartment of Cell Biology, Faculty of Biology and Environment Protection, Nicolaus Copernicus University, 87-100 Torun, Poland (K.N., J.N.).
authors.forename: Dawid
authors.identifier: 0000-0002-4322-0746
authors.initials: D
authors.lastname: Bielewicz
authors.affiliation: Department of Gene Expression, Faculty of Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, 61-614 Poznan, Poland (J.D., M.B., D.B., H.P., W.W., Z.S.-K., A.J.); andDepartment of Cell Biology, Faculty of Biology and Environment Protection, Nicolaus Copernicus University, 87-100 Torun, Poland (K.N., J.N.).
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Niedojadlo
authors.affiliation: Department of Gene Expression, Faculty of Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, 61-614 Poznan, Poland (J.D., M.B., D.B., H.P., W.W., Z.S.-K., A.J.); andDepartment of Cell Biology, Faculty of Biology and Environment Protection, Nicolaus Copernicus University, 87-100 Torun, Poland (K.N., J.N.).
authors.forename: Janusz
authors.initials: J
authors.lastname: Niedojadlo
authors.affiliation: Department of Gene Expression, Faculty of Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, 61-614 Poznan, Poland (J.D., M.B., D.B., H.P., W.W., Z.S.-K., A.J.); andDepartment of Cell Biology, Faculty of Biology and Environment Protection, Nicolaus Copernicus University, 87-100 Torun, Poland (K.N., J.N.).
authors.forename: Halina
authors.identifier: 0000-0003-0524-8067
authors.initials: H
authors.lastname: Pietrykowska
authors.affiliation: Department of Gene Expression, Faculty of Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, 61-614 Poznan, Poland (J.D., M.B., D.B., H.P., W.W., Z.S.-K., A.J.); andDepartment of Cell Biology, Faculty of Biology and Environment Protection, Nicolaus Copernicus University, 87-100 Torun, Poland (K.N., J.N.).
authors.forename: Weronika
authors.initials: W
authors.lastname: Walczak
authors.affiliation: Department of Gene Expression, Faculty of Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, 61-614 Poznan, Poland (J.D., M.B., D.B., H.P., W.W., Z.S.-K., A.J.); andDepartment of Cell Biology, Faculty of Biology and Environment Protection, Nicolaus Copernicus University, 87-100 Torun, Poland (K.N., J.N.) artjarmo@amu.edu.pl zofszwey@amu.edu.pl.
authors.forename: Zofia
authors.initials: Z
authors.lastname: Szweykowska-Kulinska
authors.affiliation: Department of Gene Expression, Faculty of Biology, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, 61-614 Poznan, Poland (J.D., M.B., D.B., H.P., W.W., Z.S.-K., A.J.); andDepartment of Cell Biology, Faculty of Biology and Environment Protection, Nicolaus Copernicus University, 87-100 Torun, Poland (K.N., J.N.) artjarmo@amu.edu.pl zofszwey@amu.edu.pl.
authors.forename: Artur
authors.initials: A
authors.lastname: Jarmolowski
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D060565:Argonaute Proteins / Q000235:genetics* / Q000378:metabolism; D015153:Blotting, Western; D002467:Cell Nucleus / Q000187:drug effects / Q000235:genetics; D015536:Down-Regulation / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics*; D030821:Plants, Genetically Modified; D020871:RNA Stability / Q000187:drug effects / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D054712:Salinity; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological
chemical_list: D029681:Arabidopsis Proteins; D060565:Argonaute Proteins; D035683:MicroRNAs; D018749:RNA, Plant; D012965:Sodium Chloride; C117457:AGO1 protein, Arabidopsis; C534587:MIRN173 microRNA, Arabidopsis; C000622838:MIRN161 microRNA, Arabidopsis