title: A one-stage cultivation process for lipid- and carbohydrate-rich biomass of Scenedesmus obtusiusculus based on artificial and natural water sources.
abstract: A one-stage cultivation process of the microalgae Scenedesmus obtusiusculus with medium based on natural water sources was developed to enhance lipids and carbohydrates. A medium based on artificial sea water, Baltic Sea water and river water with optimized nutrient concentrations compared to the standard BG-11 for nitrate (-75%), phosphate and iron (-90%) was used for cultivation. Although nitrate exhaustion over cultivation resulted in nitrate limitation, growth of the microalgae was not reduced. The lipid content increased from 6.0% to 19.9%, an increase in oleic and stearic acid was observed. The unsaponifiable matter of the lipid fraction was reduced from 19.5% to 11.4%. The carbohydrate yield rose from 45% to 50% and the protein content decreased from 32.4% to 15.9%. Using natural water sources with optimized nutrient concentrations could open the opportunity to modulate biomass composition and to reduce the cultivation costs.
authors.affiliation: Ernst-Moritz-Arndt-University of Greifswald, Institute of Pharmacy, Department Pharmaceutical Biology, Friedrich-Ludwig-Jahn-Str. 17, 17491 Greifswald, Germany. Electronic address: christian.schulze@uni-greifswald.de.
authors.forename: Christian
authors.initials: C
authors.lastname: Schulze
authors.affiliation: Ernst-Moritz-Arndt-University of Greifswald, Institute of Pharmacy, Department Pharmaceutical Biology, Friedrich-Ludwig-Jahn-Str. 17, 17491 Greifswald, Germany.
authors.forename: Jakob
authors.initials: J
authors.lastname: Reinhardt
authors.affiliation: Ernst-Moritz-Arndt-University of Greifswald, Institute of Pharmacy, Department Pharmaceutical Biology, Friedrich-Ludwig-Jahn-Str. 17, 17491 Greifswald, Germany.
authors.forename: Martina
authors.initials: M
authors.lastname: Wurster
authors.affiliation: Technische Universität München, Chair of Chemistry of Biogenic Resources, Straubing Center of Science, Schulgasse 16, Straubing, Germany.
authors.forename: José Guillermo
authors.initials: JG
authors.lastname: Ortiz-Tena
authors.affiliation: Technische Universität München, Chair of Chemistry of Biogenic Resources, Straubing Center of Science, Schulgasse 16, Straubing, Germany.
authors.forename: Volker
authors.initials: V
authors.lastname: Sieber
authors.affiliation: Ernst-Moritz-Arndt-University of Greifswald, Institute of Pharmacy, Department Pharmaceutical Biology, Friedrich-Ludwig-Jahn-Str. 17, 17491 Greifswald, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Mundt
mesh_terms: D056804:Biofuels; D018533:Biomass; D002241:Carbohydrates / Q000737:chemistry; D003470:Culture Media / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D008055:Lipids / Q000737:chemistry; D058086:Microalgae / Q000378:metabolism; D009566:Nitrates / Q000737:chemistry; D019301:Oleic Acid / Q000737:chemistry; D010710:Phosphates / Q000737:chemistry; D044444:Scenedesmus / Q000378:metabolism*; D012623:Seawater; D013229:Stearic Acids / Q000737:chemistry; D014867:Water / Q000378:metabolism*
chemical_list: D056804:Biofuels; D002241:Carbohydrates; D003470:Culture Media; D005227:Fatty Acids; D008055:Lipids; D009566:Nitrates; D010710:Phosphates; D013229:Stearic Acids; D014867:Water; D019301:Oleic Acid; C031183:stearic acid
keywords: Carbohydrates; Lipids; Medium optimization; Nitrate starvation; Scenedesmus obtusiusculus