title: TP53 codon 72 polymorphism may predict early tumour progression in paediatric pilocytic astrocytoma.
abstract: Pilocytic astrocytoma and ganglioglioma may occur in inaccessible or surgically difficult areas. In case of incomplete resection, the availability of biological predictors of tumour progression could be particularly important. To this end, an analysis of p53 codon 72 polymorphism and assessment of its role as prognostic marker were performed.The status of the p53 Arg72Pro polymorphism was evaluated by pyrosequencing method in a multicenter cohort of 170 paediatric patients. Genotype/phenotype associations were investigated either by means of bivariate or multivariate analyses.In the partially resected pilocytic astrocytomas, the Arg/Arg variant predicts early tumour progression (median survival time: 23.1 months) and is associated with poor event-free survival (p value = 0.0009). This finding remains true also in case of adjuvant therapies, with a 5-year event-free survival of 30.6% for cases with Arg/Arg variant vs. 78.7% for those with other genotypes. There is no association between ganglioglioma and the polymorphism.The assessment of Arg/Arg variant could improve the management of pilocytic astrocytoma. TP53 codon 72 analysis could distinguish low-risk cases, in which surgery could be conservative, from high-risk cases needing an aggressive surgery plan.
authors.affiliation: Dipartimento Testa, Collo e Neuroscienze, Istituto Giannina Gaslini, 16147 Genoa, Italy.
authors.forename: Samantha
authors.initials: S
authors.lastname: Mascelli
authors.affiliation: UOC Anatomia Patologica, Istituto Giannina Gaslini, 16147 Genoa, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Nozza
authors.affiliation: Division of Paediatric Neurooncology, German Cancer Research Center (DKFZ), Heidelberg 69120, Germany.
authors.forename: David T W
authors.initials: DT
authors.lastname: Jones
authors.affiliation: CRO2 UMR_S911, Inserm, Aix-Marseille Université, 13385 Marseille, France.
authors.forename: Carole
authors.initials: C
authors.lastname: Colin
authors.affiliation: Epidemiologia, Biostatistica e Comitati, Istituto Giannina Gaslini, 16147 Genoa, Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Pistorio
authors.affiliation: Dipartimento Testa, Collo e Neuroscienze, Istituto Giannina Gaslini, 16147 Genoa, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Milanaccio
authors.affiliation: Dipartimento Testa, Collo e Neuroscienze, Istituto Giannina Gaslini, 16147 Genoa, Italy.
authors.forename: Marcello
authors.initials: M
authors.lastname: Ravegnani
authors.affiliation: Dipartimento Testa, Collo e Neuroscienze, Istituto Giannina Gaslini, 16147 Genoa, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Consales
authors.affiliation: Department of Paediatric Oncology, Haematology and Immunology, University of Heidelberg, Heidelberg, 69120 Heidelberg, Germany.
authors.forename: Olaf
authors.initials: O
authors.lastname: Witt
authors.affiliation: Neuroradiologia Istituto Giannina Gaslini, 16147 Genoa, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Morana
authors.affiliation: Dipartimento Testa, Collo e Neuroscienze, Istituto Giannina Gaslini, 16147 Genoa, Italy.
authors.forename: Armando
authors.initials: A
authors.lastname: Cama
authors.affiliation: Dipartimento Testa, Collo e Neuroscienze, Istituto Giannina Gaslini, 16147 Genoa, Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Capra
authors.affiliation: Laboratory Molecular Medicine, Translational Medicine Department, Istituto Giannina Gaslini, 16147 Genoa, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Biassoni
authors.affiliation: Division of Paediatric Neurooncology, German Cancer Research Center (DKFZ), Heidelberg 69120, Germany.|Department of Paediatric Oncology, Haematology and Immunology, University of Heidelberg, Heidelberg, 69120 Heidelberg, Germany.
authors.forename: Stefan M
authors.initials: SM
authors.lastname: Pfister
authors.affiliation: CRO2 UMR_S911, Inserm, Aix-Marseille Université, 13385 Marseille, France.|APHM, Hôpital de la Timone, Service d'Anatomie Pathologique et de Neuropathologie, 13385 Marseille, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Figarella-Branger
authors.affiliation: Centro di Neuro-Oncologia, Istituto Giannina Gaslini, 16147 Genoa, Italy.
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Garrè
authors.affiliation: Dipartimento Testa, Collo e Neuroscienze, Istituto Giannina Gaslini, 16147 Genoa, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Raso
mesh_terms: D000293:Adolescent; D001254:Astrocytoma / Q000235:genetics* / Q000473:pathology; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D015331:Cohort Studies; D018450:Disease Progression; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D020641:Polymorphism, Single Nucleotide; D016019:Survival Analysis; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53
keywords: TP53; low-grade gliomas; paediatric; pilocytic astrocytoma; polymorphism