title: Unveiling of miRNA Expression Patterns in Purkinje Cells During Development.
abstract: MicroRNAs (miRNAs) are short noncoding RNAs of 19-25 nucleotides in length that regulate gene expression at the post-transcriptional level. Dysregulation of miRNAs is associated with many disorders and neurodegenerative diseases affecting numerous different pathways and processes, of which many have not yet been completely explored. Recent studies even indicate a crucial role of miRNAs during brain development, with differential expression patterns of several miRNAs seen in both developing and mature cells. A miRNA profiling in brain tissue and the fundamental understanding of their effects might optimize the therapeutical treatment of various neurological disorders. In this study, we performed miRNA array analysis of enriched cerebellar Purkinje cell (PC) samples from both young and mature rat cerebella. We used laser microdissection (LMD) to enrich PC for a highly specific miRNA profiling. Altogether, we present the expression profile of at least 27 miRNAs expressed in rat cerebellar PC and disclose a different expression pattern of at least three of these miRNAs during development. These miRNAs are potential candidates for the regulation and control of cerebellar PC development, including neuritic and dendritic outgrowth as well as spine formation.
authors.affiliation: Department of Cytology, Institute of Anatomy, Ruhr-University Bochum, Universitätsstr. 150, 44801, Bochum, Germany.
authors.forename: Lukas
authors.initials: L
authors.lastname: Pieczora
authors.affiliation: Department of Cytology, Institute of Anatomy, Ruhr-University Bochum, Universitätsstr. 150, 44801, Bochum, Germany.
authors.forename: Lara
authors.initials: L
authors.lastname: Stracke
authors.affiliation: Department of Neurology, Neuromuscular Center Ruhrgebiet, University Hospital Bergmannsheil, Ruhr-University Bochum, Buerkle-de-la-Camp-Platz 1, 44789, Bochum, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Vorgerd
authors.affiliation: Department of Molecular Gastrointestinal Oncology, Ruhr-University Bochum, Universitätsstr. 150, 44801, Bochum, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Hahn
authors.affiliation: Department of Cytology, Institute of Anatomy, Ruhr-University Bochum, Universitätsstr. 150, 44801, Bochum, Germany. carsten.theiss@ruhr-uni-bochum.de.
authors.forename: Carsten
authors.initials: C
authors.lastname: Theiss
authors.affiliation: Department of Cytology, Institute of Anatomy, Ruhr-University Bochum, Universitätsstr. 150, 44801, Bochum, Germany.
authors.forename: Verena
authors.initials: V
authors.lastname: Theis
mesh_terms: D000818:Animals; D002531:Cerebellum / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D008297:Male; D008751:Methylene Blue; D035683:MicroRNAs / Q000378:metabolism*; D046228:Microarray Analysis; D011689:Purkinje Cells / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger; D008751:Methylene Blue
keywords: Brain development; Central nervous system; Cerebellum; MicroRNA; Purkinje cell