title: Degradation of some representative polycyclic aromatic hydrocarbons by the water-soluble protein extracts from Zea mays L. cv PR32-B10.
abstract: The ability of the water-soluble protein extracts from Zea mais L. cv. PR32-B10 to degrade some representative polycyclic aromatic hydrocarbons (PAHs), has been evaluated. Surface sterilized seeds of corn (Zea mais L. Pioneer cv. PR32-B10) were hydroponically cultivated in a growth chamber under no-stressful conditions. The water-soluble protein extracts isolated from maize tissues showed peroxidase, polyphenol oxidase and catalase activities. Incubation of the extracts with naphthalene, fluorene, phenanthrene and pyrene, led to formation of oxidized and/or degradation products. GC-MS and TLC monitoring of the processes showed that naphthalene, phenanthrene, fluorene and pyrene underwent 100%, 78%, 92% and 65% oxidative degradation, respectively, after 120 min. The chemical structure of the degradation products were determined by (1)H NMR and ESI-MS spectrometry.
authors.affiliation: Department of Pharmacy, University of Naples Federico II, Via D. Montesano 49, 80131, Naples, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Barone
authors.affiliation: Department of Pharmacy, University of Naples Federico II, Via D. Montesano 49, 80131, Naples, Italy. Electronic address: margherita.debiasi@unina.it.
authors.forename: Margherita-Gabriella
authors.initials: MG
authors.lastname: de Biasi
authors.affiliation: Department of Chemical Sciences, University of Naples Federico II, Via Cyntia 4, 80126, Naples, Italy. Electronic address: vinpicci@unina.it.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Piccialli
authors.affiliation: Department of Pharmacy, University of Naples Federico II, Via D. Montesano 49, 80131, Naples, Italy.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: de Napoli
authors.affiliation: Department of Pharmacy, University of Naples Federico II, Via D. Montesano 49, 80131, Naples, Italy.
authors.forename: Giorgia
authors.initials: G
authors.lastname: Oliviero
authors.affiliation: Department of Pharmacy, University of Naples Federico II, Via D. Montesano 49, 80131, Naples, Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Borbone
authors.affiliation: Department of Pharmacy, University of Naples Federico II, Via D. Montesano 49, 80131, Naples, Italy; Institute of Protein Biochemistry, CNR, Via P. Castellino 111, 80131, Naples, Italy.
authors.forename: Gennaro
authors.initials: G
authors.lastname: Piccialli
mesh_terms: D001673:Biodegradation, Environmental; D002855:Chromatography, Thin Layer; D005449:Fluorenes / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy; D009281:Naphthalenes / Q000032:analysis; D010084:Oxidation-Reduction; D010616:Phenanthrenes / Q000032:analysis; D010936:Plant Extracts / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis*; D011721:Pyrenes / Q000032:analysis; D012989:Soil Pollutants / Q000032:analysis*; D012995:Solubility; D003313:Zea mays / Q000737:chemistry* / Q000201:enzymology / Q000378:metabolism
chemical_list: D005449:Fluorenes; D009281:Naphthalenes; D010616:Phenanthrenes; D010936:Plant Extracts; D010940:Plant Proteins; D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes; D012989:Soil Pollutants; C031721:naphthalene; C041509:fluorene; C031181:phenanthrene; C030984:pyrene
keywords: Active protein extracts; PAHs degradation; Phytoremediation; Polycyclic aromatic hydrocarbons; Zea mays