title: Nerve-independent and ectopically additional induction of taste buds in organ culture of fetal tongues.
abstract: An improved organ culture system allowed to observe morphogenesis of mouse lingual papillae and taste buds relatively for longer period, in which fetal tongues were analyzed for 6 d. Taste cells were defined as eosinophobic epithelial cells expressing CK8 and Sox2 within lingual epithelium. Addition of glycogen synthase kinase 3 beta inhibitor CHIR99021 induced many taste cells and buds in non-gustatory and gustatory stratified lingual epithelium. The present study clearly demonstrated induction of taste cells and buds ectopically and without innervation.
authors.affiliation: Graduate School of Industrial Science and Technology, Department of Biological Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo, 125-8585, Japan. hondakotaro@live.jp.
authors.forename: Kotaro
authors.initials: K
authors.lastname: Honda
authors.affiliation: Graduate School of Industrial Science and Technology, Department of Biological Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo, 125-8585, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Tomooka
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D004848:Epithelium / Q000187:drug effects / Q000294:innervation; D005333:Fetus / Q000502:physiology*; D000071679:Glycogen Synthase Kinase 3 beta / Q000037:antagonists & inhibitors / Q000378:metabolism; D053823:Hedgehog Proteins / Q000378:metabolism; D008813:Mice, Inbred ICR; D009024:Morphogenesis / Q000187:drug effects; D009417:Nerve Tissue / Q000187:drug effects / Q000502:physiology*; D009924:Organ Culture Techniques / Q000379:methods*; D054799:Phospholipase C beta / Q000378:metabolism; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D021381:Protein Transport / Q000187:drug effects; D013649:Taste / Q000187:drug effects; D013650:Taste Buds / Q000187:drug effects / Q000196:embryology*; D014059:Tongue / Q000196:embryology*; D060449:Wnt Signaling Pathway / Q000187:drug effects; D051176:beta Catenin / Q000378:metabolism
chemical_list: D053823:Hedgehog Proteins; D047428:Protein Kinase Inhibitors; C465570:Shh protein, mouse; D051176:beta Catenin; D000071679:Glycogen Synthase Kinase 3 beta; D054799:Phospholipase C beta
keywords: Morphogenesis; Organ culture; Taste bud; β-catenin