title: Tobacco Rotated with Rapeseed for Soil-Borne Phytophthora Pathogen Biocontrol: Mediated by Rapeseed Root Exudates.
abstract: Black shank, caused by Phytophthora parasitica var. nicotianae, is a widespread and destructive disease of tobacco. Crop rotation is essential in controlling black shank. Here, we confirmed that rotating black shank-infested fields with rapeseed (Brassica napus) suppressed the incidence this disease. Further study demonstrated that rapeseed roots have a strong ability to attract zoospores and subsequently stop the swimming of zoospores into cystospores. Then, rapeseed roots secrete a series of antimicrobial compounds, including 2-butenoic acid, benzothiazole, 2-(methylthio)benzothiazole, 1-(4-ethylphenyl)-ethanone, and 4-methoxyindole, to inhibit the cystospore germination and mycelial growth of P. parasitica var. nicotianae. Thus, rapeseed rotated with tobacco suppresses tobacco black shank disease through the chemical weapons secreted by rapeseed roots.
authors.affiliation: State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan UniversityKunming, China; Key Laboratory for Agro-biodiversity and Pest Control of Ministry of Education, Yunnan Agricultural UniversityKunming, China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Fang
authors.affiliation: Yunnan Tobacco Company, Yuxi Branch Yuxi, China.
authors.forename: Limeng
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Yunnan Tobacco Company, Yuxi Branch Yuxi, China.
authors.forename: Yongge
authors.initials: Y
authors.lastname: Jiao
authors.affiliation: State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan UniversityKunming, China; Key Laboratory for Agro-biodiversity and Pest Control of Ministry of Education, Yunnan Agricultural UniversityKunming, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Liao
authors.affiliation: State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan UniversityKunming, China; Key Laboratory for Agro-biodiversity and Pest Control of Ministry of Education, Yunnan Agricultural UniversityKunming, China.
authors.forename: Lifen
authors.initials: L
authors.lastname: Luo
authors.affiliation: Yunnan Tobacco Company, Yuxi Branch Yuxi, China.
authors.forename: Sigui
authors.initials: S
authors.lastname: Ji
authors.affiliation: Yunnan Tobacco Company, Yuxi Branch Yuxi, China.
authors.forename: Jiangzhou
authors.initials: J
authors.lastname: Li
authors.affiliation: Yunnan Tobacco Company, Yuxi Branch Yuxi, China.
authors.forename: Kuai
authors.initials: K
authors.lastname: Dai
authors.affiliation: State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan UniversityKunming, China; Key Laboratory for Agro-biodiversity and Pest Control of Ministry of Education, Yunnan Agricultural UniversityKunming, China.
authors.forename: Shusheng
authors.initials: S
authors.lastname: Zhu
authors.affiliation: State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan UniversityKunming, China; Key Laboratory for Agro-biodiversity and Pest Control of Ministry of Education, Yunnan Agricultural UniversityKunming, China.
authors.forename: Min
authors.initials: M
authors.lastname: Yang
keywords: Phytophthora parasitica; chemical interaction; root exudates; rotation; soil-borne pathogen