title: Semisolid liver infusion tryptose supplemented with human urine allows growth and isolation of Trypanosoma cruzi and Trypanosoma rangeli clonal lineages.
abstract: BACKGROUND
  This work shows that 3% (v/v) human urine (HU) in semisolid Liver Infusion Tryptose (SSL) medium favors the growth of Trypanosoma cruzi and T. rangeli.
  
  
  METHODS
  Parasites were plated as individual or mixed strains on SSL medium and on SSL medium with 3% human urine (SSL-HU). Isolate DNA was analyzed using polymerase chain reaction (PCR) and pulsed-field gel electrophoresis (PFGE).
  
  
  RESULTS
  SSL-HU medium improved clone isolation. PCR revealed that T. cruzi strains predominate on mixed-strain plates. PFGE confirmed that isolated parasites share the same molecular karyotype as parental cell lines.
  
  
  CONCLUSIONS
  SSL-HU medium constitutes a novel tool for obtaining T. cruzi and T. rangeli clonal lineages.
authors.affiliation: Instituto de Ciências Biológicas e Naturais, Universidade Federal do Triângulo Mineiro, Uberaba, Minas Gerais, Brasil.
authors.forename: Emanuella Francisco
authors.initials: EF
authors.lastname: Fajardo
authors.affiliation: Instituto de Ciências da Saúde, Universidade Federal do Triângulo Mineiro, Uberaba, Minas Gerais, Brasil.
authors.forename: Marlene
authors.initials: M
authors.lastname: Cabrine-Santos
authors.affiliation: Centro de Educação Profissional, Universidade Federal do Triângulo Mineiro, Uberaba, Minas Gerais, Brasil.
authors.forename: Keila Adriana Magalhães
authors.initials: KA
authors.lastname: Ferreira
authors.affiliation: Instituto de Ciências Biológicas e Naturais, Universidade Federal do Triângulo Mineiro, Uberaba, Minas Gerais, Brasil.
authors.forename: Eliane
authors.initials: E
authors.lastname: Lages-Silva
authors.affiliation: Instituto de Ciências Biológicas e Naturais, Universidade Federal do Triângulo Mineiro, Uberaba, Minas Gerais, Brasil.
authors.forename: Luis Eduardo
authors.initials: LE
authors.lastname: Ramírez
authors.affiliation: Instituto de Ciências Biológicas e Naturais, Universidade Federal do Triângulo Mineiro, Uberaba, Minas Gerais, Brasil.
authors.forename: André Luiz
authors.initials: AL
authors.lastname: Pedrosa
mesh_terms: D003470:Culture Media / Q000737:chemistry*; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D059785:Karyotype; D009930:Organic Chemicals / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D014349:Trypanosoma cruzi / Q000235:genetics / Q000254:growth & development*; D057509:Trypanosoma rangeli / Q000235:genetics / Q000254:growth & development*; D014556:Urine / Q000737:chemistry*
chemical_list: D003470:Culture Media; D009930:Organic Chemicals; C046666:tryptose