title: Preparation study of indocyanine green-rituximab: A new receptor-targeted tracer for sentinel lymph node in breast cancer.
abstract: An appropriate receptor-targeted tracer for sentinel lymph node biopsy (SLNB) was prepared. We combined the fluorescence tracer (Indocyanine green, ICG) with Rituximab (a chimeric human/murine monoclonal antibody targeting the CD20 antigen on the surface of lymphocyte) directly to produce a new tracer (ICG-Rituximab). When the new tracer drains to the lymph node, Rituximab will combine with CD20 receptor on the B-cell surface in the lymph node. If the statue of antibody-receptor connection does not reach saturation, the number of Rituximab is less than CD20. With this appropriate injection dose, the new tracer could only stay in sentinel lymph node (SLN) and make it imaging. Positive fluorescence SLN was detected 12 minutes after injection with no other organs imaging. The imaging of SLN was stable and clear for 20-24 hours. Due to SLN stained with more ICG than the lymphatic vessel, the fluorescence situation of SLN would be brighter than the vessel. The surgeon can detect the positive fluorescence SLN easily without following the fluorescence imaging lymphatic vessel. The results of our preliminary study showed that the new tracer might be useful for improving SLN imaging and worth further clinical study. SLNB with the new tracer could be a convenient method for detecting SLN and would become a standard performance in clinical practice.
authors.affiliation: School of Medicine and Life Sciences, University of Jinan and Shandong Academy of Medical Sciences, Jinan, 250200, China.|Breast Cancer Center, Shandong Cancer Hospital Affiliated to Shandong University, Jinan, 250117, China.
authors.forename: Bin-Bin
authors.initials: BB
authors.lastname: Cong
authors.affiliation: Breast Cancer Center, Shandong Cancer Hospital Affiliated to Shandong University, Jinan, 250117, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Sun
authors.affiliation: Basic Laboratory, Shandong Cancer Hospital Affiliated to Shandong University, Jinan, 250117, China.
authors.forename: Xian-Rang
authors.initials: XR
authors.lastname: Song
authors.affiliation: Breast Cancer Center, Shandong Cancer Hospital Affiliated to Shandong University, Jinan, 250117, China.
authors.forename: Yan-Bing
authors.initials: YB
authors.lastname: Liu
authors.affiliation: Breast Cancer Center, Shandong Cancer Hospital Affiliated to Shandong University, Jinan, 250117, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Zhao
authors.affiliation: School of Medicine and Life Sciences, University of Jinan and Shandong Academy of Medical Sciences, Jinan, 250200, China.|Breast Cancer Center, Shandong Cancer Hospital Affiliated to Shandong University, Jinan, 250117, China.
authors.forename: Xiao-Shan
authors.initials: XS
authors.lastname: Cao
authors.affiliation: Breast Cancer Center, Shandong Cancer Hospital Affiliated to Shandong University, Jinan, 250117, China.
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Qiu
authors.affiliation: School of Medicine and Life Sciences, University of Jinan and Shandong Academy of Medical Sciences, Jinan, 250200, China.|Breast Cancer Center, Shandong Cancer Hospital Affiliated to Shandong University, Jinan, 250117, China.
authors.forename: Chong-Lin
authors.initials: CL
authors.lastname: Tian
authors.affiliation: Radiotherapy Department, Shandong Cancer Hospital Affiliated to Shandong University, Jinan, 250117, China.
authors.forename: Jin-Ming
authors.initials: JM
authors.lastname: Yu
authors.affiliation: Breast Cancer Center, Shandong Cancer Hospital Affiliated to Shandong University, Jinan, 250117, China.
authors.forename: Yong-Sheng
authors.initials: YS
authors.lastname: Wang
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000473:pathology*; D005260:Female; D005453:Fluorescence; D007208:Indocyanine Green; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D000069283:Rituximab / Q000378:metabolism*; D000071036:Sentinel Lymph Node / Q000000981:diagnostic imaging*; D021701:Sentinel Lymph Node Biopsy / Q000379:methods*
chemical_list: D000069283:Rituximab; D007208:Indocyanine Green
keywords: indocyanine green; lymphoscintigraphy; rituximab; sentinel lymph node biopsy; synthetic imaging agent