title: [Application of mass spectrometry in mycology].
abstract: MALDI-TOF (matrix-assisted laser desorption ionization time-of-flight) mass spectrometry (MS) is becoming an essential tool in most microbiology laboratories. At present, by using a characteristic fungal profile obtained from whole cells or through simple extraction protocols, MALDI-TOF MS allows the identification of pathogenic fungi with a high performance potential. This methodology decreases the laboratory turnaround time, optimizing the detection of mycoses. This article describes the state-of-the-art of the use of MALDI-TOF MS for the detection of human clinical fungal pathogens in the laboratory and discusses the future applications of this technology, which will further improve routine mycological diagnosis.
authors.affiliation: Servicio de Microbiología, Hospital Universitario La Paz, Madrid, España.
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Quiles Melero
authors.affiliation: Servicio de Microbiología, Hospital Universitario Gregorio Marañón, Madrid, España.
authors.forename: Teresa
authors.initials: T
authors.lastname: Peláez
authors.affiliation: Servicio de Microbiología, Hospital Universitario Miguel Servet, Zaragoza, España.
authors.forename: Antonio
authors.initials: A
authors.lastname: Rezusta López
authors.affiliation: Servicio de Microbiología, Hospital Universitario La Paz, Madrid, España. Electronic address: juiiogarciarodriguez@gmail.com.
authors.forename: Julio
authors.initials: J
authors.lastname: Garcia-Rodríguez
mesh_terms: D003955:Diagnostic Tests, Routine; D005544:Forecasting; D016469:Fungemia / Q000175:diagnosis / Q000382:microbiology; D005658:Fungi / Q000302:isolation & purification*; D006801:Humans; D016533:Mycological Typing Techniques; D009172:Mycology / Q000379:methods* / Q000639:trends; D009181:Mycoses / Q000175:diagnosis* / Q000382:microbiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods* / Q000639:trends
keywords: Fungi; Hongos; Identificación; Identification; MALDI-TOF; Micología; Mycology