title: Analysis of the complete genome sequence of black queen cell virus JL1 from infected honeybees in China.
abstract: There are six strains of the complete genomic sequences of black queen cell virus (BQCV) published in the GenBank, including South Africa (AF183905), South Korea (JX149531), Hungary 10 (EF517515), Poland 4 (EF517519), Poland 5 (EF517520) and Poland 6 (EF517521). Based on the six BQCV strains published in the GenBank, ten pairs of primers were designed in the present study using reverse transcription polymerase chain reaction to obtain the first complete genome sequence of a BQCV strain in China, called the BQCV China-JL1 strain (KP119603). A phylogenetic tree was then built to analyse their genetic relationships. The BQCV China-JL1 strain showed 86-93% similarity with the six strains published in the GenBank. The BQCV China-JL1 strain consisted of 8358 nucleotides (nt). The 5'-proximal open reading frame (ORF1) initiated at nt position 546 and terminated at nt position 4676, ORF3 initiated at nt position 4891 and terminated at nt position 5433, and the 3'-proximal ORF (ORF2) was located between nt positions 5750 and 8203.
authors.affiliation: JiLin Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China,Changchun 130062,China.
authors.forename: Q
authors.initials: Q
authors.lastname: Yang
authors.affiliation: JiLin Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China,Changchun 130062,China.
authors.forename: Z-Y
authors.initials: ZY
authors.lastname: Song
authors.affiliation: College Veterinary Medicine Jilin University,Changchun 130062,China.
authors.forename: X
authors.initials: X
authors.lastname: Feng
authors.affiliation: Changchun Institute of Biological Products Co., Ltd.,Changchun 130062,China.
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.affiliation: JiLin Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China,Changchun 130062,China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: JiLin Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China,Changchun 130062,China.
authors.forename: X-H
authors.initials: XH
authors.lastname: Wang
authors.affiliation: JiLin Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China,Changchun 130062,China.
authors.forename: J-C
authors.initials: JC
authors.lastname: Sui
authors.affiliation: JiLin Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China,Changchun 130062,China.
authors.forename: Z-G
authors.initials: ZG
authors.lastname: Wang
authors.affiliation: JiLin Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China,Changchun 130062,China.
authors.forename: Y
authors.initials: Y
authors.lastname: Sun
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001516:Bees / Q000821:virology*; D002681:China; D056629:Dicistroviridae / Q000235:genetics*; D010802:Phylogeny; D058974:Phylogeography; D012367:RNA, Viral / Q000737:chemistry; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein
chemical_list: D012367:RNA, Viral
keywords: BQCV; RT–PCR; RdRp; complete genome sequence; phylogenetic analysis