title: L929 fibroblast bioassay on the in vitro toxicity of SnCl2, H3PO4, Clearfil SE primer and combinations thereof.
abstract: Stannous chloride (SnCl(2), 35%) can increase microtensile bond strength between a self-etching adhesive and dental hard tissue either alone or in combination with phosphoric acid (H(3)PO(4), 35%). Whereas cell toxicity of H(3)PO(4) has been sufficiently investigated, little is known about the toxicity of concentrated SnCl(2). The present study determined the in vitro toxicity of SnCl(2), H(3)PO(4), the primer of a self-etching adhesive (Clearfil SE) and combinations thereof at three concentrations (0.01%0.3%) in a L929 fibroblast bioassay. Cell viability was determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, the integrity of cell membranes was visualised by trypan blue staining (cell necrosis assay). Cell viability was impaired at concentrations between 0.09% and 0.13% for SnCl(2), between 0.06% and 0.09% for H(3)PO(4), and between 0.19% and 0.29% for Clearfil SE primer. Combinations of agents showed additive toxic effects. SnCl(2) showed a slightly lower but comparable in vitro toxicity to H(3)PO(4), which gives a perspective for further in vitro, in situ and clinical studies on this issue, and for the intraoral use of SnCl(2) to increase bond strength between an adhesive system and both enamel and dentine.
authors.affiliation: Department of Preventive, Restorative and Pediatric Dentistry, University of Bern, Bern, Switzerland.
authors.forename: Nadine
authors.initials: N
authors.lastname: Schlueter
authors.forename: Adrian
authors.initials: A
authors.lastname: Lussi
authors.forename: Carolina
authors.initials: C
authors.lastname: Ganss
authors.forename: Reinhard
authors.initials: R
authors.lastname: Gruber
mesh_terms: D000134:Acid Etching, Dental; D001681:Biological Assay; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D004338:Drug Combinations; D006801:Humans; D066298:In Vitro Techniques; D010756:Phosphoric Acids / Q000633:toxicity*; D019279:Resin Cements / Q000633:toxicity*; D017971:Tin Compounds / Q000633:toxicity*
chemical_list: C438313:Clearfil SE Bond; D004338:Drug Combinations; D010756:Phosphoric Acids; D019279:Resin Cements; D017971:Tin Compounds; C023599:stannous chloride; C030242:phosphoric acid
keywords: L929; MTT; SnCl2; trypan blue; adhesive dentistry