title: Antibacterial and antifouling activities of chitosan/TiO2/Ag NPs nanocomposite films against packaged drinking water bacterial isolates.
abstract: TiO2 and Ag NPs are widely used as antibacterial agents against many bacterial pathogens. Chitosan (polymer) itself acts as a strong antibacterial agent. Hence, chitosan/TiO2/Ag NPs incorporated nanocomposite film was prepared against packed drinking water bacterial strains. A concentration-dependent increase in the reduction of cell viability was observed in all the isolates under UV-C and dark exposure conditions. The bacteria consortium showed greater resistance against antibacterial effects of chitosan/TiO2/Ag nanocomposite as compared to single isolates. Glycocalyx test and mass assessment conclude the effective antibacterial activity by inhibiting bacterial adhesion on the film surface. The release of LDH and generation of ROS act as the predominant antibacterial mechanism induced by TiO2/Ag NPs. Surface characterization of chitosan/TiO2/Ag nanocomposite was studied by FTIR and XRD analyses and SEM analysis after interaction with the bacteria.
authors.affiliation: Centre for Nanobiotechnology, VIT University, Vellore, 632 014, India.
authors.forename: Saravanan
authors.initials: S
authors.lastname: Natarajan
authors.affiliation: Centre for Nanobiotechnology, VIT University, Vellore, 632 014, India.
authors.forename: M
authors.initials: M
authors.lastname: Bhuvaneshwari
authors.affiliation: Reverse Osmosis Membrane Division, CSIR-Central Salt and Marine Chemicals Research Institute 10 (CSIR-CSMCRI), Council of Scientific and Industrial Research (CSIR), G. B. Marg, 11, Bhavnagar, 364 002, Gujarat, India. slakshmi@csmcri.org.
authors.forename: D Shanthana
authors.initials: DS
authors.lastname: Lakshmi
authors.affiliation: Centre for Nanobiotechnology, VIT University, Vellore, 632 014, India.
authors.forename: P
authors.initials: P
authors.lastname: Mrudula
authors.affiliation: Centre for Nanobiotechnology, VIT University, Vellore, 632 014, India.
authors.forename: N
authors.initials: N
authors.lastname: Chandrasekaran
authors.affiliation: Centre for Nanobiotechnology, VIT University, Vellore, 632 014, India. amit.mookerjea@gmail.com.
authors.forename: Amitava
authors.identifier: http://orcid.org/0000-0001-8682-4278
authors.initials: A
authors.lastname: Mukherjee
mesh_terms: D000900:Anti-Bacterial Agents / Q000633:toxicity*; D001419:Bacteria / Q000187:drug effects; D048271:Chitosan / Q000633:toxicity*; D060766:Drinking Water / Q000737:chemistry / Q000382:microbiology*; D053761:Nanocomposites / Q000633:toxicity*; D011108:Polymers; D012834:Silver / Q000633:toxicity*; D014025:Titanium / Q000633:toxicity*; D018508:Water Purification / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D060766:Drinking Water; D011108:Polymers; C009495:titanium dioxide; D012834:Silver; D048271:Chitosan; D014025:Titanium
keywords: Antibacterial activity; Chitosan/TiO2/Ag nanocomposite; Consortium; Dark; UV-C