title: Pretransplant Immune- and Apoptosis-Related Gene Expression Is Associated with Kidney Allograft Function.
abstract: Renal transplant candidates present immune dysregulation, caused by chronic uremia. The aim of the study was to investigate whether pretransplant peripheral blood gene expression of immune factors affects clinical outcome of renal allograft recipients. Methods. In a prospective study, we analyzed pretransplant peripheral blood gene expression in87 renal transplant candidates with real-time PCR on custom-designed low density arrays (TaqMan). Results. Immediate posttransplant graft function (14-day GFR) was influenced negatively by TGFB1 (P = 0.039) and positively by IL-2 gene expression (P = 0.040). Pretransplant blood mRNA expression of apoptosis-related genes (CASP3, FAS, and IL-18) and Th1-derived cytokine gene IFNG correlated positively with short- (6-month GFR CASP3: P = 0.027, FAS: P = 0.021, and IFNG: P = 0.029) and long-term graft function (24-month GFR CASP3: P = 0.003, FAS: P = 0.033, IL-18: P = 0.044, and IFNG: P = 0.04). Conclusion. Lowered pretransplant Th1-derived cytokine and apoptosis-related gene expressions were a hallmark of subsequent worse kidney function but not of acute rejection rate. The pretransplant IFNG and CASP3 and FAS and IL-18 genes' expression in the recipients' peripheral blood is the possible candidate for novel biomarker of short- and long-term allograft function.
authors.affiliation: Department of Nephrology and Transplantation Medicine, Wroclaw Medical University, 52-129 Wrocław, Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Kamińska
authors.affiliation: Department of Nephrology and Transplantation Medicine, Wroclaw Medical University, 52-129 Wrocław, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kościelska-Kasprzak
authors.affiliation: Department of Vascular, General and Transplant Surgery, Wroclaw Medical University, 52-129 Wrocław, Poland.
authors.forename: Paweł
authors.initials: P
authors.lastname: Chudoba
authors.affiliation: Department of Nephrology and Transplantation Medicine, Wroclaw Medical University, 52-129 Wrocław, Poland.
authors.forename: Oktawia
authors.initials: O
authors.lastname: Mazanowska
authors.affiliation: Department of Nephrology and Transplantation Medicine, Wroclaw Medical University, 52-129 Wrocław, Poland.
authors.forename: Mirosław
authors.initials: M
authors.lastname: Banasik
authors.affiliation: Department of Nephrology and Transplantation Medicine, Wroclaw Medical University, 52-129 Wrocław, Poland.
authors.forename: Marcelina
authors.initials: M
authors.lastname: Żabinska
authors.affiliation: Department of Nephrology and Transplantation Medicine, Wroclaw Medical University, 52-129 Wrocław, Poland.
authors.forename: Maria
authors.initials: M
authors.lastname: Boratyńska
authors.affiliation: Department of Vascular, General and Transplant Surgery, Wroclaw Medical University, 52-129 Wrocław, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Lepiesza
authors.affiliation: Department of Histology and Embryology, Wroclaw Medical University, 50-368 Wrocław, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Gomółkiewicz
authors.affiliation: Department of Histology and Embryology, Wroclaw Medical University, 50-368 Wrocław, Poland.
authors.forename: Piotr
authors.initials: P
authors.lastname: Dzięgiel
authors.affiliation: Department of Nephrology and Transplantation Medicine, Wroclaw Medical University, 52-129 Wrocław, Poland.
authors.forename: Marian
authors.initials: M
authors.lastname: Klinger
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D053148:Caspase 3 / Q000097:blood; D016207:Cytokines / Q000097:blood; D005260:Female; D006084:Graft Rejection / Q000097:blood / Q000188:drug therapy / Q000276:immunology; D006801:Humans; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D007371:Interferon-gamma / Q000097:blood; D020382:Interleukin-18 / Q000097:blood; D007376:Interleukin-2 / Q000097:blood; D016030:Kidney Transplantation / Q000009:adverse effects*; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D012333:RNA, Messenger / Q000097:blood; D053773:Transforming Growth Factor beta1 / Q000097:blood; D014184:Transplantation, Homologous / Q000009:adverse effects*; D055815:Young Adult; D019014:fas Receptor / Q000097:blood
chemical_list: D016207:Cytokines; C491901:FAS protein, human; C573415:IFNG protein, human; D007166:Immunosuppressive Agents; D020382:Interleukin-18; D007376:Interleukin-2; D012333:RNA, Messenger; C508960:TGFB1 protein, human; D053773:Transforming Growth Factor beta1; D019014:fas Receptor; D007371:Interferon-gamma; D053148:Caspase 3