title: Untargeted Metabolomics of Tomato Plants after Root-Knot Nematode Infestation.
abstract: After 2 months from the infestation of tomato plants with the root-knot nematode (RKN) Meloidogyne incognita, we performed a gas chromatography-mass spectrometry untargeted fingerprint analysis for the identification of characteristic metabolites and biomarkers. Principal component analysis, and orthogonal projections to latent structures discriminant analysis suggested dramatic local changes of the plant metabolome. In the case of tomato leaves, β-alanine, phenylalanine, and melibiose were induced in response to RKN stimuli, while ribose, glycerol, myristic acid, and palmitic acid were reduced. For tomato stems, upregulated metabolites were ribose, sucrose, fructose, and glucose, while fumaric acid and glycine were downregulated. The variation in molecular strategies to the infestation of RKNs may play an important role in how Solanum lycopersicum and other plants adapt to nematode parasitic stress.
authors.affiliation: Department of Life and Environmental Sciences, University of Cagliari , via Ospedale 72, 09124 Cagliari, Italy.
authors.forename: Kodjo
authors.initials: K
authors.lastname: Eloh
authors.affiliation: Institute for Sustainable Plant Protection, National Research Council (CNR) , via G. Amendola 122/D, 70126 Bari, Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Sasanelli
authors.affiliation: Department of Life and Environmental Sciences, University of Cagliari , via Ospedale 72, 09124 Cagliari, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Maxia
authors.affiliation: Department of Life and Environmental Sciences, University of Cagliari , via Ospedale 72, 09124 Cagliari, Italy.
authors.forename: Pierluigi
authors.initials: P
authors.lastname: Caboni
mesh_terms: D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry; D018551:Solanum lycopersicum / Q000737:chemistry* / Q000378:metabolism* / Q000469:parasitology; D055432:Metabolomics; D010935:Plant Diseases / Q000469:parasitology*; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism / Q000469:parasitology; D014431:Tylenchoidea / Q000469:parasitology*
keywords: Meloidogyne incognita; acylsugars; metabonomics; plant defense response; plant metabolism