title: Immobilization of an artificial imine reductase within silica nanoparticles improves its performance.
abstract: Silica nanoparticles equipped with an artificial imine reductase display remarkable activity towards cyclic imine- and NAD(+) reduction. The method, based on immobilization and protection of streptavidin on silica nanoparticles, shields the biotinylated metal cofactor against deactivation yielding over 46 000 turnovers in pure samples and 4000 turnovers in crude cellular extracts.
authors.affiliation: Department of Chemistry, University of Basel, Spitalstrasse 51, CH-4056 Basel, Switzerland. thomas.ward@unibas.ch.
authors.forename: Martina
authors.initials: M
authors.lastname: Hestericová
authors.forename: M Rita
authors.initials: MR
authors.lastname: Correro
authors.forename: Markus
authors.initials: M
authors.lastname: Lenz
authors.forename: Philippe F-X
authors.initials: PF
authors.lastname: Corvini
authors.forename: Patrick
authors.initials: P
authors.lastname: Shahgaldian
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Ward