title: Engineering a glycerol utilization pathway in Corynebacterium glutamicum for succinate production under O2 deprivation.
abstract: OBJECTIVE
  To explore the glycerol utilization pathway in Corynebacterium glutamicum for succinate production under O2 deprivation.
  
  
  RESULTS
  Overexpression of a glycerol facilitator, glycerol dehydrogenase and dihydroxyacetone kinase from Escherichia coli K-12 in C. glutamicum led to recombinant strains NC-3G diverting glycerol utilization towards succinate production under O2 deprivation. Under these conditions, strain NC-3G efficiently consumed glycerol and produced succinate without growth. The recombinant C. glutamicum utilizing glycerol as the sole carbon source showed higher intracellular NADH/NAD(+) ratio compare with utilizing glucose. The mass conversion of succinate increased from 0.64 to 0.95. Using an anaerobic fed-batch fermentation process, the final strain produced 38.4 g succinate/l with an average yield of 1.02 g/g.
  
  
  CONCLUSIONS
  The metabolically-engineered strains showed an efficient succinate production using glycerol as sole carbon source under O2 deprivation.
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, People's Republic of China.
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, People's Republic of China. cheng@njut.edu.cn.
authors.forename: Heng
authors.initials: H
authors.lastname: Cai
authors.affiliation: College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot, People's Republic of China.
authors.forename: Zhongjun
authors.initials: Z
authors.lastname: Chen
authors.affiliation: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, People's Republic of China.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Zhou
mesh_terms: D000693:Anaerobiosis; D061249:Batch Cell Culture Techniques; D048230:Corynebacterium glutamicum / Q000235:genetics* / Q000502:physiology; D048168:Escherichia coli K12 / Q000201:enzymology / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism*; D060847:Metabolic Engineering / Q000379:methods; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics* / Q000378:metabolism; D013386:Succinates / Q000378:metabolism*; D013401:Sugar Alcohol Dehydrogenases / Q000235:genetics* / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D013386:Succinates; D013401:Sugar Alcohol Dehydrogenases; C020672:glycerol dehydrogenase; D017853:Phosphotransferases (Alcohol Group Acceptor); C023132:glycerone kinase; D005990:Glycerol
keywords: Corynebacterium glutamicum; Glycerol utilization; NADH generation; Succinate production