title: Visinin-Like Protein-3 Modulates the Interaction Between Cytochrome b5and NADH-Cytochrome b5Reductase in a Ca2+-Dependent Manner.
abstract: Visinin-like proteins (VILIPs) belong to the calcium sensor protein family. VILIP-1 has been examined as a cerebrospinal fluid biomarker and as a potential indicator for cognitive decline in Alzheimer's disease (AD). However, little is known about VILIP-3 protein biochemistry. We performed co-immunoprecipitation experiments to examine whether VILIP-3 can interact with reduced nicotine adenine dinucleotide (NADH)-cytochrome b5reductase. We also evaluated the specificity of cytochrome b5within the visinin-like protein subfamily and identified cytochrome P450 isoforms in the brain. In this study, we show that cytochrome b5has an affinity for hippocalcin, neurocalcin-δ, and VILIP-3, but not visinin-like protein-1. VILIP-3 was also shown to interact with NADH-cytochrome b5reductase in a Ca2+-dependent manner. These results suggest that VILIP-3, hippocalcin, and neurocalcin-δ provide a Ca2+-dependent modulation to the NADH-dependent microsomal electron transport. The results also suggest that future therapeutic strategies that target calcium-signaling pathways and VILIPs may be of value.
authors.affiliation: Division of Neurodegenerative Disorders, St. Boniface Hospital Research, 351 Tache Ave./Room 4050, Winnipeg, MB, R2H 2A6, Canada.
authors.forename: Kensuke
authors.initials: K
authors.lastname: Oikawa
authors.affiliation: Division of Neurodegenerative Disorders, St. Boniface Hospital Research, 351 Tache Ave./Room 4050, Winnipeg, MB, R2H 2A6, Canada.
authors.forename: Gary L
authors.initials: GL
authors.lastname: Odero
authors.affiliation: Division of Neurodegenerative Disorders, St. Boniface Hospital Research, 351 Tache Ave./Room 4050, Winnipeg, MB, R2H 2A6, Canada.|Department of Pharmacology and Therapeutics, University of Manitoba, Winnipeg, Canada.
authors.forename: Solmaz
authors.initials: S
authors.lastname: Nafez
authors.affiliation: Division of Neurodegenerative Disorders, St. Boniface Hospital Research, 351 Tache Ave./Room 4050, Winnipeg, MB, R2H 2A6, Canada.
authors.forename: Ning
authors.initials: N
authors.lastname: Ge
authors.affiliation: Division of Neurodegenerative Disorders, St. Boniface Hospital Research, 351 Tache Ave./Room 4050, Winnipeg, MB, R2H 2A6, Canada.
authors.forename: Dali
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Pathology, and School of Nursing, Asahikawa Medical College, Asahikawa, 078-8510, Japan.
authors.forename: Hiroya
authors.initials: H
authors.lastname: Kobayashi
authors.affiliation: Department of Pathology, and School of Nursing, Asahikawa Medical College, Asahikawa, 078-8510, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Sate
authors.affiliation: Department of Pathology, and School of Nursing, Asahikawa Medical College, Asahikawa, 078-8510, Japan.
authors.forename: Shoji
authors.initials: S
authors.lastname: Kimura
authors.affiliation: Department of Pathology, and School of Nursing, Asahikawa Medical College, Asahikawa, 078-8510, Japan.
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Tateno
authors.affiliation: Division of Neurodegenerative Disorders, St. Boniface Hospital Research, 351 Tache Ave./Room 4050, Winnipeg, MB, R2H 2A6, Canada. balbensi@sbrc.ca.|Department of Pharmacology and Therapeutics, University of Manitoba, Winnipeg, Canada. balbensi@sbrc.ca.
authors.forename: Benedict C
authors.initials: BC
authors.lastname: Albensi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism; D002118:Calcium / Q000737:chemistry / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D042926:Cytochrome P-450 CYP4A / Q000378:metabolism; D042966:Cytochrome-B(5) Reductase / Q000737:chemistry / Q000378:metabolism*; D015786:Cytochromes b5 / Q000737:chemistry / Q000378:metabolism*; D057809:HEK293 Cells; D051597:Hippocalcin / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D047468:Immunoprecipitation; D007477:Ions / Q000737:chemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D051596:Neurocalcin / Q000737:chemistry / Q000378:metabolism; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D016415:Sequence Alignment
chemical_list: D002135:Calcium-Binding Proteins; D007477:Ions; D009419:Nerve Tissue Proteins; D051596:Neurocalcin; C485222:VILIP-3 protein, mouse; D051597:Hippocalcin; D015786:Cytochromes b5; D042926:Cytochrome P-450 CYP4A; D042966:Cytochrome-B(5) Reductase; D002118:Calcium
keywords: Calcium; Calcium sensor; Electron transport; Mitochondria; Molecular; Nerve