title: The p22 RNA Silencing Suppressor of the Crinivirus Tomato chlorosis virus is Dispensable for Local Viral Replication but Important for Counteracting an Antiviral RDR6-Mediated Response during Systemic Infection.
abstract: Among the components of the RNA silencing pathway in plants, RNA-dependent RNA polymerases (RDRs) play fundamental roles in antiviral defence. Here, we demonstrate that the Nicotiana benthamiana RDR6 is involved in defence against the bipartite crinivirus (genus Crinivirus, family Closteroviridae) Tomato chlorosis virus (ToCV). Additionally, by producing a p22-deficient ToCV infectious mutant clone (ToCVΔp22), we studied the role of this viral suppressor of RNA silencing in viral infection in both wild-type and RDR6-silenced N. benthamiana (NbRDR6i) plants. We demonstrate that p22 is dispensable for the replication of ToCV, where RDR6 appears not to have any effect. Furthermore, the finding that ToCV∆p22 systemic accumulation was impaired in wild-type N. benthamiana but not in NbRDR6i plants suggests a role for p22 in counteracting an RDR6-mediated antiviral response of the plant during systemic infection.
authors.affiliation: Instituto de Hortofruticultura Subtropical y Mediterr&#225;nea "La Mayora"-Universidad de M&#225;laga-Consejo Superior de Investigaciones Cient&#237;ficas (IHSM-UMA-CSIC), Estaci&#243;n Experimental "La Mayora", 29750 Algarrobo-Costa, M&#225;laga, Spain. landeoyazmin@gmail.com.
authors.forename: Yazmín
authors.initials: Y
authors.lastname: Landeo-Ríos
authors.affiliation: Instituto de Hortofruticultura Subtropical y Mediterr&#225;nea "La Mayora"-Universidad de M&#225;laga-Consejo Superior de Investigaciones Cient&#237;ficas (IHSM-UMA-CSIC), Estaci&#243;n Experimental "La Mayora", 29750 Algarrobo-Costa, M&#225;laga, Spain. jnavas@eelm.csic.es.
authors.forename: Jesús
authors.initials: J
authors.lastname: Navas-Castillo
authors.affiliation: Instituto de Hortofruticultura Subtropical y Mediterr&#225;nea "La Mayora"-Universidad de M&#225;laga-Consejo Superior de Investigaciones Cient&#237;ficas (IHSM-UMA-CSIC), Estaci&#243;n Experimental "La Mayora", 29750 Algarrobo-Costa, M&#225;laga, Spain. moriones@eelm.csic.es.
authors.forename: Enrique
authors.initials: E
authors.lastname: Moriones
authors.affiliation: Instituto de Hortofruticultura Subtropical y Mediterr&#225;nea "La Mayora"-Universidad de M&#225;laga-Consejo Superior de Investigaciones Cient&#237;ficas (IHSM-UMA-CSIC), Estaci&#243;n Experimental "La Mayora", 29750 Algarrobo-Costa, M&#225;laga, Spain. carmen.canizares@eelm.csic.es.
authors.forename: M Carmen
authors.initials: MC
authors.lastname: Cañizares
mesh_terms: D029383:Crinivirus / Q000276:immunology* / Q000502:physiology*; D054884:Host-Pathogen Interactions; D057131:Immune Evasion; D010935:Plant Diseases / Q000276:immunology / Q000821:virology; D010940:Plant Proteins / Q000378:metabolism; D034622:RNA Interference; D014026:Nicotiana / Q000276:immunology* / Q000821:virology; D014764:Viral Proteins / Q000378:metabolism; D014779:Virus Replication
chemical_list: D010940:Plant Proteins; D014764:Viral Proteins
keywords: Agroinoculation; Closteroviridae; Crinivirus; Infectious clone; RNA silencing suppressor; Tomato chlorosis virus