title: RecA stimulates AlkB-mediated direct repair of DNA adducts.
abstract: The Escherichia coli AlkB protein is a 2-oxoglutarate/Fe(II)-dependent demethylase that repairs alkylated single stranded and double stranded DNA. Immunoaffinity chromatography coupled with mass spectrometry identified RecA, a key factor in homologous recombination, as an AlkB-associated protein. The interaction between AlkB and RecA was validated by yeast two-hybrid assay; size-exclusion chromatography and standard pull down experiment and was shown to be direct and mediated by the N-terminal domain of RecA. RecA binding results AlkB-RecA heterodimer formation and RecA-AlkB repairs alkylated DNA with higher efficiency than AlkB alone.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Hyderabad, Kandi, 502285 Hyderabad, Telangana, India.
authors.forename: Gururaj
authors.initials: G
authors.lastname: Shivange
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Hyderabad, Kandi, 502285 Hyderabad, Telangana, India.
authors.forename: Mohan
authors.initials: M
authors.lastname: Monisha
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Hyderabad, Kandi, 502285 Hyderabad, Telangana, India.
authors.forename: Richa
authors.initials: R
authors.lastname: Nigam
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Hyderabad, Kandi, 502285 Hyderabad, Telangana, India.
authors.forename: Naveena
authors.initials: N
authors.lastname: Kodipelli
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Hyderabad, Kandi, 502285 Hyderabad, Telangana, India anindya@iith.ac.in.
authors.forename: Roy
authors.initials: R
authors.lastname: Anindya
mesh_terms: D000071496:AlkB Enzymes / Q000737:chemistry / Q000378:metabolism*; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism; D018736:DNA Adducts; D019175:DNA Methylation; D004260:DNA Repair; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D008968:Molecular Conformation; D010084:Oxidation-Reduction; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D011938:Rec A Recombinases / Q000737:chemistry / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D018736:DNA Adducts; D000071496:AlkB Enzymes; D011938:Rec A Recombinases