title: Comparison of the regiospecific distribution from triacylglycerols after chemical and enzymatic interesterification of high oleic sunflower oil and fully hydrogenated high oleic sunflower oil blend by carbon-13 nuclear magnetic resonance.
abstract: The nutritional and organoleptic attributes of oils can proceed via interesterification of oils blends catalyzed by enzymes or chemicals. Enzymatic interesterification processes are preferred due the regiospecific outcome. Traditionally, monitoring of distribution of fatty acids (FA) in glycerol backbone is performed by enzymatic and chromatographic methods that are time-consuming, involving a series of chemical manipulations employing large volumes of organic solvents. Alternatively, carbon-13 nuclear magnetic resonance ((13)C NMR) is a fast and reliable technique that could be applied to determine the saturated and unsaturated FA distribution of the triacylglycerols (TAGs) present in high oleic sunflower oil (SO) and fully hydrogenated high oleic sunflower oil (HSO) blends and their interesterification products. The enzymatic interesterification was conducted employing the immobilized lipase from Thermomyces lanuginosus (Lipozyme TL IM), the results show that the process was not completely regiospecific at sn-1,3 positions, due to the spontaneous acyl migration from position sn-2 to sn-1,3.
authors.affiliation: State University of Campinas - UNICAMP, Chemistry Institute, Campinas, 13083-970 São Paulo, Brazil.
authors.forename: Thiago I B
authors.initials: TI
authors.lastname: Lopes
authors.affiliation: State University of Campinas - UNICAMP, Faculty of Food Engineering, Campinas, 13081-970 São Paulo, Brazil.
authors.forename: Marilene D M M
authors.initials: MD
authors.lastname: Ribeiro
authors.affiliation: State University of Campinas - UNICAMP, Faculty of Food Engineering, Campinas, 13081-970 São Paulo, Brazil.
authors.forename: Chiu C
authors.initials: CC
authors.lastname: Ming
authors.affiliation: State University of Campinas - UNICAMP, Faculty of Food Engineering, Campinas, 13081-970 São Paulo, Brazil.
authors.forename: Renato
authors.initials: R
authors.lastname: Grimaldi
authors.affiliation: State University of Campinas - UNICAMP, Faculty of Food Engineering, Campinas, 13081-970 São Paulo, Brazil.
authors.forename: Lireny A G
authors.initials: LA
authors.lastname: Gonçalves
authors.affiliation: State University of Campinas - UNICAMP, Chemistry Institute, Campinas, 13083-970 São Paulo, Brazil. Electronic address: anita@iqm.unicamp.br.
authors.forename: Anita J
authors.initials: AJ
authors.lastname: Marsaioli
mesh_terms: D001203:Ascomycota / Q000201:enzymology; D002247:Carbon Isotopes / Q000737:chemistry; D002384:Catalysis; D004800:Enzymes, Immobilized / Q000737:chemistry; D004951:Esterification; D005227:Fatty Acids / Q000737:chemistry*; D005231:Fatty Acids, Unsaturated / Q000737:chemistry*; D005583:Fourier Analysis; D006859:Hydrogen; D006865:Hydrogenation; D008049:Lipase / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D010938:Plant Oils / Q000032:analysis*; D012997:Solvents / Q000737:chemistry; D013237:Stereoisomerism; D000074242:Sunflower Oil; D014280:Triglycerides / Q000737:chemistry*
chemical_list: D002247:Carbon Isotopes; D004800:Enzymes, Immobilized; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D010938:Plant Oils; D012997:Solvents; D000074242:Sunflower Oil; D014280:Triglycerides; D006859:Hydrogen; C093666:Lipozyme; D008049:Lipase
keywords: (13)C NMR; High oleic sunflower oil; Interesterification; Regiospecificity