title: Production of Biodegradable Polyester by a Transgenic Tobacco.
abstract: The acetoacetyl-CoA reductase gene (phbB) of Ralstonia eutropha and the poly[(R)-(-)-3-hydroxyalkanoate] synthase gene (phaCAC) of Aeromonas caviae were introduced into tobacco plant by Agrobacterium mediated transformation method. The resulting transgenic tobacco expressed both introduced genes and the expression of these genes was confirmed by enzymatic analysis and western blotting. GC-MS analysis of the chloroform extract of tobacco leaves indicated that the transgenic plant produced biodegradable polyester, poly-[(R)-(-)-3-hydroxybutyrate]. GPC analysis indicated that the number-average molecular weights (Mn) and polydispersity (Mw/Mn) were 32,000 and 1.90, respectively.
authors.affiliation: a The Institute of Physical and Chemical Research (RIKEN).
authors.forename: H
authors.initials: H
authors.lastname: Nakashita
authors.affiliation: b Toyo University.
authors.forename: Y
authors.initials: Y
authors.lastname: Arai
authors.affiliation: a The Institute of Physical and Chemical Research (RIKEN).
authors.forename: K
authors.initials: K
authors.lastname: Yoshioka
authors.affiliation: a The Institute of Physical and Chemical Research (RIKEN).
authors.forename: T
authors.initials: T
authors.lastname: Fukui
authors.affiliation: a The Institute of Physical and Chemical Research (RIKEN).
authors.forename: Y
authors.initials: Y
authors.lastname: Doi
authors.affiliation: b Toyo University.
authors.forename: R
authors.initials: R
authors.lastname: Usami
authors.affiliation: b Toyo University.
authors.forename: K
authors.initials: K
authors.lastname: Horikoshi
authors.affiliation: a The Institute of Physical and Chemical Research (RIKEN).
authors.forename: I
authors.initials: I
authors.lastname: Yamaguchi
keywords: Aeromonas caviae; Agrobacterium; PHA; polyhydroxybutyrate; transgenic plant