title: A Novel Catechol Oxidase Enzyme Electrode for the Specific Determination of Catechol.
abstract: An enzyme electrode for the specific determination of catechol was developed by using catechol oxidase (EC 1.10.3.1) from eggplant (Solanum melangena L.) in combination with a dissolved oxygen probe. Optimization studies of the prepared catechol oxidase enzyme electrode established a phosphate buffer 50 mM at pH 7.0 and 35°C to provide the optimum conditions for affirmative electrode response. The enzyme electrode response depended linearly on a catechol concentration range of 5•10(-7)-30•10(-5) M with a response time of 25 sec and substrate specificity of the catechol oxidase electrode of 100%. The biosensor retained its enzyme activity for at least 70 days.
authors.affiliation: a Biochemistry Department, Faculty of Science, Ege University.
authors.forename: E
authors.initials: E
authors.lastname: Dinçkaya
authors.affiliation: a Biochemistry Department, Faculty of Science, Ege University.
authors.forename: E
authors.initials: E
authors.lastname: Akyilmaz
authors.affiliation: a Biochemistry Department, Faculty of Science, Ege University.
authors.forename: S
authors.initials: S
authors.lastname: Akgöl
authors.affiliation: a Biochemistry Department, Faculty of Science, Ege University.
authors.forename: S
authors.initials: S
authors.lastname: Tatar Önal
authors.affiliation: a Biochemistry Department, Faculty of Science, Ege University.
authors.forename: F
authors.initials: F
authors.lastname: Zihnioglu
authors.affiliation: a Biochemistry Department, Faculty of Science, Ege University.
authors.forename: A
authors.initials: A
authors.lastname: Telefoncu
keywords: biosensor; catechol; eggplant (Solanum melangena L.); enzyme electrode