title: Transcriptome sequencing and comparative analysis of Schizochytrium mangrovei PQ6 at different cultivation times.
abstract: OBJECTIVE
  The heterotrophic marine microalga, Schizochytrium mangrovei PQ6, synthesizes large amounts of polyunsaturated fatty acids (PUFAs) with possible nutritional applications. We characterized the transcriptome of S. mangrovei PQ6, focusing on lipid metabolism pathways throughout growth.
  
  
  RESULTS
  Cell growth, total lipid, and docosahexaenoic acid (DHA, 22:6n-3) contents of S. mangrovei PQ6 in 500 ml batch cultures rapidly increased on day 1 in cultivation and reached their maximum levels on day 5. Maximum lipid accumulation in 500 ml batch cultures occurred on day 5, with total lipid and DHA contents reaching 33.2 ± 1.25% of dry cell weight (DCW) and 136 mg/g DCW, respectively. 11,025 unigenes, 28,617 unigenes and 18,480 unigenes from the transcriptomes of samples collected on day 1, 3, and 5 in cultivation were identified, respectively. These unigenes of the three samples were further assembled into 30,782 unigenes with an average size of 673 bp and N50 of 950 bp, and a total of 9,980 unigenes were annotated in public protein databases. 93 unigenes involved in lipid metabolism in which expression patterns corresponded with total lipid and DHA accumulation patterns were identified.
  
  
  CONCLUSIONS
  The possible roles of PUFAs pathways, such as those mediated by fatty acid synthase, polyketide synthase, and desaturase/elongase, co-exist in S. mangrovei PQ6.
authors.affiliation: Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam.
authors.forename: Minh Hien
authors.initials: MH
authors.lastname: Hoang
authors.affiliation: Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam.
authors.forename: Cuong
authors.initials: C
authors.lastname: Nguyen
authors.affiliation: Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam.
authors.forename: Huy Quang
authors.initials: HQ
authors.lastname: Pham
authors.affiliation: Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam.
authors.forename: Lam
authors.initials: L
authors.lastname: Van Nguyen
authors.affiliation: Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam.
authors.forename: Le
authors.initials: L
authors.lastname: Hoang Duc
authors.affiliation: Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam.
authors.forename: Le
authors.initials: L
authors.lastname: Van Son
authors.affiliation: Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam.
authors.forename: Truong Nam
authors.initials: TN
authors.lastname: Hai
authors.affiliation: Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam.
authors.forename: Chu Hoang
authors.initials: CH
authors.lastname: Ha
authors.affiliation: Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam.
authors.forename: Lam Dai
authors.initials: LD
authors.lastname: Nhan
authors.affiliation: Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam.
authors.forename: Hoang Thi Lan
authors.initials: HT
authors.lastname: Anh
authors.affiliation: Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam.
authors.forename: Le Thi
authors.initials: le T
authors.lastname: Thom
authors.affiliation: Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam.
authors.forename: Hoang Thi Huong
authors.initials: HT
authors.lastname: Quynh
authors.affiliation: Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam.
authors.forename: Nguyen Cam
authors.initials: NC
authors.lastname: Ha
authors.affiliation: Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam.
authors.forename: Pham
authors.initials: P
authors.lastname: Van Nhat
authors.affiliation: Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam. ddhong60vn@yahoo.com.
authors.forename: Dang Diem
authors.initials: DD
authors.lastname: Hong
mesh_terms: D020418:Algal Proteins / Q000235:genetics / Q000378:metabolism; D061249:Batch Cell Culture Techniques; D004281:Docosahexaenoic Acids / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D053263:Gene Regulatory Networks; D050356:Lipid Metabolism; D058977:Molecular Sequence Annotation; D048030:RNA, Algal / Q000032:analysis; D017423:Sequence Analysis, RNA / Q000379:methods*; D058009:Stramenopiles / Q000235:genetics / Q000254:growth & development*
chemical_list: D020418:Algal Proteins; D048030:RNA, Algal; D004281:Docosahexaenoic Acids
keywords: De novo assembly; Docosahexaenoic acid; Lipid biosynthesis; Schizochytrium mangrovei; Transcriptome