title: Fabrication of BSA-Green Tea Polyphenols-Chitosan Nanoparticles and Their Role in Radioprotection: A Molecular and Biochemical Approach.
abstract: Normal tissue damage from ionizing radiation during radiotherapy is a major concern in cancer treatment. Tea polyphenols (TPs) have been shown to reduce radiation-induced damage in multiple studies, but their pharmacological application is still limited due to poor bioavailability. The present study was aimed at to increase the TPs bioavailability by nanoformulation by using BSA as the matrix and chitosan as the external shell. Encapsulated TPs nanoparticles were spherical in size and promoted TPs stability in normal and gastrointestinal conditions without losing antioxidant activity. Oral administration of nanoparticles for 3 days prior to irradiation exposure has been shown to protect mice from hematological injuries that result in the reduction of radiation-induced lethality. TPs reduce radiation-induced oxidative damage and apoptosis by restoring the redox status through the Nrf2-ERK pathway and reducing Bax expression, respectively. Regarding potency, encapsulated TPs have shown a significantly higher level of radioprotection than TPs, suggesting that TP nanoparticles can be explored as valuable radioprotective and pharmacotherapeutic agent.
authors.affiliation: School of Life Science and ‡School of Environmental Sciences, Jawaharlal Nehru University , New Delhi 110067, India.
authors.forename: Sumit
authors.initials: S
authors.lastname: Kumar
authors.affiliation: School of Life Science and ‡School of Environmental Sciences, Jawaharlal Nehru University , New Delhi 110067, India.
authors.forename: Ramovatar
authors.initials: R
authors.lastname: Meena
authors.affiliation: School of Life Science and ‡School of Environmental Sciences, Jawaharlal Nehru University , New Delhi 110067, India.
authors.forename: Paulraj
authors.initials: P
authors.lastname: Rajamani
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants; D017209:Apoptosis / Q000187:drug effects; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry; D004355:Drug Stability; D008297:Male; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D059808:Polyphenols / Q000737:chemistry*; D011833:Radiation Injuries, Experimental / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000737:chemistry* / Q000627:therapeutic use*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013154:Spleen / Q000528:radiation effects; D013662:Tea / Q000737:chemistry
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D059808:Polyphenols; D011837:Radiation-Protective Agents; D013662:Tea; D012710:Serum Albumin, Bovine; D048271:Chitosan
keywords: antioxidants; free-radical scavenging; nanoparticle; radiation protection; tea polyphenols