title: Experimental study on the inhibition effect of miR-106a inhibitor on tumor growth of ovarian cancer xenografts mice.
abstract: OBJECTIVE
  To study the inhibition effect of miR-106a inhibitor on tumor growth of ovarian cancer xenografts mice.
  
  
  METHODS
  BALB/c mice were selected as experimental animals, ovarian cancer SKOV-3 cells transfected with miR-106a inhibitor and its negative control were inoculated subcutaneously, intratumoral injection of miR-106a inhibitor and its negative control were continued after tumor formation, and they were enrolled as treatment group and model group, respectively. Tumor volume and weight as well as Ki-67 and programmed cell death 4 (PDCD4) expression were determined; miR-106a inhibitor and its negative control as well as miR-106a mimic and its negative control were transfected into SKOV-3 cells, and expression of PDCD4 in cells was determined.
  
  
  RESULTS
  Tumor tissue volume and weight as well as mRNA expression and protein expression of Ki-67 in treatment group were significantly lower than those in the model group while mRNA expression and protein expression of PDCD4 were significantly higher than those in the model group; transfection of miR-106a mimic could decrease mRNA expression and protein expression of PDCD4 in SKOV-3 cells, and transfection of miR-106a inhibitor could increase mRNA expression and protein expression of PDCD4 in SKOV-3 cells.
  
  
  CONCLUSIONS
  Transfection of miR-106a inhibitor can inhibit the growth of tumor in ovarian cancer xenografts mice through increasing the expression of PDCD4.
authors.affiliation: Gynecology Department, Affiliated Hospital of Hebei University, Baoding City 071000, Hebei Province, China.
authors.forename: Zhi-Hui
authors.initials: ZH
authors.lastname: Cai
authors.affiliation: Gynecology Department, Affiliated Hospital of Hebei University, Baoding City 071000, Hebei Province, China. Electronic address: 304153929@qq.com.
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Chen
authors.affiliation: Gynecology Department, Affiliated Hospital of Hebei University, Baoding City 071000, Hebei Province, China.
authors.forename: Yi-Juan
authors.initials: YJ
authors.lastname: Liang
authors.affiliation: Gynecology Department, Affiliated Hospital of Hebei University, Baoding City 071000, Hebei Province, China.
authors.forename: Jun-Rong
authors.initials: JR
authors.lastname: Shi
authors.affiliation: Gynecology Department, Affiliated Hospital of Hebei University, Baoding City 071000, Hebei Province, China.
authors.forename: M A
authors.initials: MA
authors.lastname: You-Ju
authors.affiliation: Gynecology Department, Affiliated Hospital of Hebei University, Baoding City 071000, Hebei Province, China.
authors.forename: Wei-Ming
authors.initials: WM
authors.lastname: Wang
authors.affiliation: Gynecology and Obstetrics Department, Jingxiu District Hospital of Baoding City, Baoding City 071000, Hebei Province, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Yang
keywords: Ovarian cancer; Programmed cell death 4; Xenografts; miR-106a