title: Aorta Atherosclerosis Lesion Analysis in Hyperlipidemic Mice.
abstract: Atherosclerosis is a chronic inflammatory disease of large and medium-sized arteries. Apolipoprotein E-deficient (ApoE-/-) mice are used as experimental models to study human atherosclerosis. ApoE-/-mice are constitutively hyperlipidemic and develop intima plaques that resemble human plaques. Various issues including experimental design for lesion analysis, dietary conditions, isolation of the aorta, staining methods, morphometry, group size, age, the location within the arterial tree, and statistical analyses are important parameters that need to be addressed to obtain robust data. Here, we provide detailed methods to quantify aorta atherosclerosis.
authors.affiliation: Institute for Cardiovascular Prevention, Ludwig-Maximilians-University, Munich, Germany.
authors.forename: Sarajo
authors.initials: S
authors.lastname: Mohanta
authors.affiliation: Institute for Cardiovascular Prevention, Ludwig-Maximilians-University, Munich, Germany.
authors.forename: Changjun
authors.initials: C
authors.lastname: Yin
authors.affiliation: Institute for Cardiovascular Prevention, Ludwig-Maximilians-University, Munich, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Weber
authors.affiliation: Institute of Molecular Immunology, Helmholtz Zentrum München, Munich, Germany; Institute for Immunology, Ludwig-Maximilians-University, Munich, Germany.
authors.forename: Desheng
authors.initials: D
authors.lastname: Hu
authors.affiliation: Institute for Cardiovascular Prevention, Ludwig-Maximilians-University, Munich, Germany.
authors.forename: Andreas Jr
authors.initials: AJ
authors.lastname: Habenicht