title: Identification of species and genetic variation in Taenia isolates from human and swine of North India.
abstract: Taenia solium is the major cause of taeniasis and cysticercosis/neurocysticercosis (NCC) in the developing countries including India, but the existence of other Taenia species and genetic variation have not been studied in India. So, we studied the existence of different Taenia species, and sequence variation in Taenia isolates from human (proglottids and cysticerci) and swine (cysticerci) in North India. Amplification of cytochrome c oxidase subunit 1 gene (cox1) was done by polymerase chain reaction (PCR) followed by sequencing and phylogenetic analysis. We identified two species of Taenia i.e. T. solium and Taenia asiatica in our isolates. T. solium isolates showed similarity with Asian genotype and nucleotide variations from 0.25 to 1.01 %, whereas T. asiatica displayed nucleotide variations ranged from 0.25 to 0.5 %. These findings displayed the minimal genetic variations in North Indian isolates of T. solium and T. asiatica.
authors.affiliation: Department of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, 226 014, Uttar Pradesh, India.
authors.forename: Satyendra K
authors.initials: SK
authors.lastname: Singh
authors.affiliation: Department of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, 226 014, Uttar Pradesh, India. knpusha2002@yahoo.com.
authors.forename: Kashi N
authors.initials: KN
authors.lastname: Prasad
authors.affiliation: Department of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, 226 014, Uttar Pradesh, India.
authors.forename: Aloukick K
authors.initials: AK
authors.lastname: Singh
authors.affiliation: Department of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, 226 014, Uttar Pradesh, India.
authors.forename: Kamlesh K
authors.initials: KK
authors.lastname: Gupta
authors.affiliation: Department of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, 226 014, Uttar Pradesh, India.
authors.forename: Ranjeet S
authors.initials: RS
authors.lastname: Chauhan
authors.affiliation: Department of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, 226 014, Uttar Pradesh, India.
authors.forename: Amrita
authors.initials: A
authors.lastname: Singh
authors.affiliation: Department of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, 226 014, Uttar Pradesh, India.
authors.forename: Avinash
authors.initials: A
authors.lastname: Singh
authors.affiliation: Department of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, 226 014, Uttar Pradesh, India.
authors.forename: Ravi P
authors.initials: RP
authors.lastname: Rai
authors.affiliation: Department of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, 226 014, Uttar Pradesh, India.
authors.forename: Binod K
authors.initials: BK
authors.lastname: Pati
mesh_terms: D000818:Animals; D003551:Cysticercosis / Q000453:epidemiology / Q000469:parasitology*; D003552:Cysticercus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007194:India / Q000453:epidemiology; D020019:Neurocysticercosis / Q000453:epidemiology / Q000469:parasitology*; D010802:Phylogeny; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000469:parasitology*; D013621:Taenia / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D041201:Taenia solium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D013622:Taeniasis / Q000453:epidemiology / Q000469:parasitology*
keywords: Cytochrome c oxidase subunit 1 gene; Sequence variation; Taenia species