title: CYP1A1 mediates the suppression of major inflammatory cytokines in pulmonary alveolar macrophage (PAM) cell lines caused by Mycoplasma hyponeumoniae.
abstract: Mycoplasmal pneumonia is a lung infection disease caused by Mycoplasma hyopneumoniae in swine. We previously reported that Cytochrome P450 1A1 (CYP1A1) expression was significantly downregulated in pigs infected with M. hyopneumoniae compared to the healthy controls. In this study, pulmonary alveolar macrophage (PAM) cell lines with CYP1A1 overexpression or siRNA-mediated CYP1A1 silencing were used to explore the biological function and regulatory mechanism of CYP1A1 gene expression changed on the inflammatory response of pigs infected with M. hyopneumoniae. The results showed that the cells overexpressing CYP1A1 infected with M. hyopneumoniae led to a rapid increase in PPAR-γ expression, which resulted in decreasing the levels of several inflammatory cytokines including IL-1β, IL-6, IL-8 and TNF-α. On the contrary, this effect was just opposite in CYP1A1-RNAi cells infected with M. hyopneumoniae. We suggest that CYP1A1 suppress the inflammatory response caused by M. hyopneumoniae infection, via PPAR-γ signaling pathway in pigs.
authors.affiliation: Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, 50 Zhongling Street, Nanjing 210014, China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Fang
authors.affiliation: Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, 50 Zhongling Street, Nanjing 210014, China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, 50 Zhongling Street, Nanjing 210014, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xu
authors.affiliation: Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, 50 Zhongling Street, Nanjing 210014, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Tu
authors.affiliation: Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, 50 Zhongling Street, Nanjing 210014, China.
authors.forename: Xuemin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, 50 Zhongling Street, Nanjing 210014, China.
authors.forename: Bixia
authors.initials: B
authors.lastname: Li
authors.affiliation: Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, 50 Zhongling Street, Nanjing 210014, China.
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, 50 Zhongling Street, Nanjing 210014, China. Electronic address: shouwenren@163.com.
authors.forename: Shouwen
authors.initials: S
authors.lastname: Ren
mesh_terms: D000818:Animals; D002460:Cell Line; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism; D005786:Gene Expression Regulation; D007165:Immunosuppression Therapy; D018836:Inflammation Mediators / Q000378:metabolism; D016676:Macrophages, Alveolar / Q000276:immunology* / Q000382:microbiology; D045705:Mycoplasma hyopneumoniae / Q000276:immunology*; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D011019:Pneumonia, Mycoplasma / Q000276:immunology*; D034741:RNA, Small Interfering / Q000235:genetics; D015398:Signal Transduction; D013552:Swine / Q000276:immunology*
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D047495:PPAR gamma; D034741:RNA, Small Interfering; D019363:Cytochrome P-450 CYP1A1
keywords: CYP1A1; Inflammatory response; Mycoplasma hyopneumoniae; Pig