title: A Major Locus for Quantitatively Measured Shank Skin Color Traits in Korean Native Chicken.
abstract: Shank skin color of Korean native chicken (KNC) shows large color variations. It varies from white, yellow, green, bluish or grey to black, whilst in the majority of European breeds the shanks are typically yellow-colored. Three shank skin color-related traits (i.e., lightness [L*], redness [a*], and yellowness [b*]) were measured by a spectrophotometer in 585 progeny from 68 nuclear families in the KNC resource population. We performed genome scan linkage analysis to identify loci that affect quantitatively measured shank skin color traits in KNC. All these birds were genotyped with 167 DNA markers located throughout the 26 autosomes. The SOLAR program was used to conduct multipoint variance-component quantitative trait locus (QTL) analyses. We detected a major QTL that affects b* value (logarithm of odds [LOD] = 47.5, p = 1.60×10-49) on GGA24 (GGA forGallus gallus). At the same location, we also detected a QTL that influences a* value (LOD = 14.2, p = 6.14×10-16). Additionally, beta-carotene dioxygenase 2 (BCDO2), the obvious positional candidate gene under the linkage peaks on GGA24, was investigated by the two association tests: i.e., measured genotype association (MGA) and quantitative transmission disequilibrium test (QTDT). Significant associations were detected betweenBCDO2g.9367 A>C and a* (PMGA= 1.69×10-28;PQTDT= 2.40×10-25). The strongest associations were betweenBCDO2g.9367 A>C and b* (PMGA= 3.56×10-66;PQTDT= 1.68×10-65). However, linkage analyses conditional on the single nucleotide polymorphism indicated that other functional variants should exist. Taken together, we demonstrate for the first time the linkage and association between theBCDO2locus on GGA24 and quantitatively measured shank skin color traits in KNC.
authors.affiliation: Division of Animal and Dairy Science, Chungnam National University, Deajeon 34134, Korea.
authors.forename: S
authors.initials: S
authors.lastname: Jin
authors.affiliation: Division of Animal and Dairy Science, Chungnam National University, Deajeon 34134, Korea.
authors.forename: J H
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Division of Animal and Dairy Science, Chungnam National University, Deajeon 34134, Korea.
authors.forename: D W
authors.initials: DW
authors.lastname: Seo
authors.affiliation: Division of Animal and Dairy Science, Chungnam National University, Deajeon 34134, Korea.|Department of Animal Science, Faculty of Agriculture, Sebelas Maret University, Surakarta 57126, Indonesia.
authors.forename: M
authors.initials: M
authors.lastname: Cahyadi
authors.affiliation: Division of Animal and Dairy Science, Chungnam National University, Deajeon 34134, Korea.
authors.forename: N R
authors.initials: NR
authors.lastname: Choi
authors.affiliation: Poultry Research Institute, National Institute of Animal Science, Rural Development Administration, Cheonan 31000, Korea.
authors.forename: K N
authors.initials: KN
authors.lastname: Heo
authors.affiliation: Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Research Institute for Agriculture and Life Science, Seoul National University, Seoul 08826, Korea.
authors.forename: C
authors.initials: C
authors.lastname: Jo
authors.affiliation: Division of Animal and Dairy Science, Chungnam National University, Deajeon 34134, Korea.|Subtropical Livestock Research Institute, National Institute of Animal Science, Jeju 63242, Korea.
authors.forename: H B
authors.initials: HB
authors.lastname: Park
keywords: BCDO2; Korean Native Chicken; Linkage and Association; Quantitative Trait Locus; Shank Skin Color