title: Recurrent amplification of RTEL1 and ABCA13 and its synergistic effect associated with clinicopathological data of gastric adenocarcinoma.
abstract: BACKGROUND
  Despite progression in treatment of gastric cancer, prognosis of patients remains poor, in part due to the low rate of diagnosis during its early stages. This paradigm implies the necessity to identify molecular biomarkers for early gastric cancer diagnosis, as well as for disease monitoring, thus contributing to the development of new therapeutic approaches. In a previous study, performed by array-Comparative Genomic Hybridization, we described for the first time in literature recurrent amplification of RTEL1 and ABCA13 genes in gastric cancer. Thus, the aim of the present study was to validate recurrent amplification of RTEL1 and ABCA13 genes and associate CNV status with clinicopathological data.
  
  
  RESULTS
  Results showed RTEL1 and ABCA13 amplification in 38 % of samples. Statistical analysis demonstrated that RTEL amplification is more common in older patients and more associated with intestinal type and ABCA13 amplification increases the risk of lymph node metastasis and is more common in men. Co-amplification of these genes showed a significant association with advanced staging.
  
  
  CONCLUSIONS
  aCGH is a very useful tool for investigating novel genes associated with carcinogenesis and RTEL1 amplification may be important for the development of gastric cancer in older patients, besides being a probable event contributing for chromosomal instability in intestinal gastric carcinogenesis. ABCA13 amplification may have age-specific function and could be considered a useful marker for predicting lymph node metastasis in resected gastric cancer patients in early stage. Lastly, RTEL1 and ABCA13 synergistic effect may be considered as a putative marker for advanced staging in gastric cancer patients.
authors.affiliation: Human Cytogenetics Laboratory, Federal University of Pará, Belém, 66075-110 Pará Brazil.|Oncology Research Center, Federal University of Pará, Belém, 66073-000 Pará Brazil.
authors.forename: T M
authors.initials: TM
authors.lastname: Araújo
authors.affiliation: Human Cytogenetics Laboratory, Federal University of Pará, Belém, 66075-110 Pará Brazil.|Oncology Research Center, Federal University of Pará, Belém, 66073-000 Pará Brazil.
authors.forename: A D
authors.initials: AD
authors.lastname: Seabra
authors.affiliation: Molecular Biology Department, Federal University of Paraíba, João Pessoa, 58051-900 Paraíba Brazil.
authors.forename: E M
authors.initials: EM
authors.lastname: Lima
authors.affiliation: Human Cytogenetics Laboratory, Federal University of Pará, Belém, 66075-110 Pará Brazil.|Oncology Research Center, Federal University of Pará, Belém, 66073-000 Pará Brazil.
authors.forename: P P
authors.initials: PP
authors.lastname: Assumpção
authors.affiliation: Human Cytogenetics Laboratory, Federal University of Pará, Belém, 66075-110 Pará Brazil.|Oncology Research Center, Federal University of Pará, Belém, 66073-000 Pará Brazil.
authors.forename: R C
authors.initials: RC
authors.lastname: Montenegro
authors.affiliation: Oncology Research Center, Federal University of Pará, Belém, 66073-000 Pará Brazil.
authors.forename: S
authors.initials: S
authors.lastname: Demachki
authors.affiliation: Human Cytogenetics Laboratory, Federal University of Pará, Belém, 66075-110 Pará Brazil.|Oncology Research Center, Federal University of Pará, Belém, 66073-000 Pará Brazil.
authors.forename: R M
authors.initials: RM
authors.lastname: Burbano
authors.affiliation: Human Cytogenetics Laboratory, Federal University of Pará, Belém, 66075-110 Pará Brazil.|Oncology Research Center, Federal University of Pará, Belém, 66073-000 Pará Brazil.
authors.forename: A S
authors.initials: AS
authors.lastname: Khayat
keywords: ABCA13; Biomarkers; Gastric cancer; RTEL1; aCGH