title: Bioconversion of AHX to AOH by resting cells of Burkholderia contaminans CH-1.
abstract: Fairy rings are zones of stimulated grass growth owing to the interaction between a fungus and a plant. We previously reported the discovery of two novel plant-growth regulating compounds related to forming fairy rings, 2-azahypoxanthine (AHX) and 2-aza-8-oxohypoxanthine (AOH). In this study, a bacterial strain CH-1 was isolated from an airborne-contaminated nutrient medium containing AHX. The strain converted AHX to AOH and identified as Burkholderia contaminans based on the gene sequence of its 16S rDNA. The quantitative production of AOH by resting cells of the strain was achieved. Among seven Burkholderia species, two bacteria and two yeasts tested, B. contaminans CH-1 showed the highest rate of conversion of AHX to AOH. By batch system, up to 10.6 mmol AHX was converted to AOH using the resting cells. The yield of this process reached at 91%.
authors.affiliation: a College of Agriculture , Academic Institute, Shizuoka University , Shizuoka , Japan.|b Graduate School of Agriculture , Shizuoka University , Shizuoka , Japan.|c Research Institute of Green Science and Technology , Shizuoka University , Shizuoka , Japan.
authors.forename: Jae-Hoon
authors.initials: JH
authors.lastname: Choi
authors.affiliation: b Graduate School of Agriculture , Shizuoka University , Shizuoka , Japan.
authors.forename: Ayaka
authors.initials: A
authors.lastname: Kikuchi
authors.affiliation: b Graduate School of Agriculture , Shizuoka University , Shizuoka , Japan.
authors.forename: Panyapon
authors.initials: P
authors.lastname: Pumkaeo
authors.affiliation: a College of Agriculture , Academic Institute, Shizuoka University , Shizuoka , Japan.|b Graduate School of Agriculture , Shizuoka University , Shizuoka , Japan.|c Research Institute of Green Science and Technology , Shizuoka University , Shizuoka , Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Hirai
authors.affiliation: a College of Agriculture , Academic Institute, Shizuoka University , Shizuoka , Japan.|b Graduate School of Agriculture , Shizuoka University , Shizuoka , Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Tokuyama
authors.affiliation: a College of Agriculture , Academic Institute, Shizuoka University , Shizuoka , Japan.|b Graduate School of Agriculture , Shizuoka University , Shizuoka , Japan.|c Research Institute of Green Science and Technology , Shizuoka University , Shizuoka , Japan.|d Graduate School of Science and Technology , Shizuoka University , Shizuoka , Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Kawagishi
mesh_terms: D000391:Air Microbiology; D001711:Biotransformation; D019117:Burkholderia / Q000166:cytology* / Q000302:isolation & purification / Q000378:metabolism*; D007042:Hypoxanthines / Q000378:metabolism*
chemical_list: C000591883:2-aza-8-oxohypoxanthine; D007042:Hypoxanthines; C012759:2-azahypoxanthine
keywords: Burkholderia contaminans; bioconversion; fairy chemicals; fairy ring; plant growth regulator