title: Electrosynthesis and binding properties of molecularly imprinted poly-o-phenylenediamine for selective recognition and direct electrochemical detection of myoglobin.
abstract: Electrosynthesis of molecularly imprinted polymer (MIP) templated with myoglobin (Mb) and the reference non-imprinted polymer (NIP) was examined with o-phenylenediamine (o-PD) as a monomer. Mass-sensitive quartz crystal microbalance with dissipation monitoring supplied by an electrochemical module (EQCM-D) was applied to characterize and optimize MIP/NIP electrosynthesis. Mb rebinding was detected by direct electrocatalytic reduction of Mb by square wave voltammetry (SWV) or differential pulse voltammetry (DPV). The results obtained showed high specificity of polymeric antibodies to template Mb, with an imprinting factor determined as a ratio Imax(MIP)/Imax(NIP) of 2-4. The prepared MIP sensor is characterized by an apparent dissociation constant of (3.3±0.5)×10(-9)M and has a broad range of working concentrations of 1nM-1μМ, with the detection limit of 0.5nM (9ng/ml). Mb rebinding was examined in Mb-free diluted human serum spiked with Mb as well as in plasma samples of patients with acute myocardial infarction (AMI) and in control plasma of healthy donors in order to demonstrate the potential medical application of developed MIP sensors.
authors.affiliation: Institute of Biomedical Chemistry, 119121 Moscow, Russia; IBMC-EcoBioPharm Company, 119121 Moscow, Russia; N.I. Pirogov Russian National Medical University, 117997 Moscow, Russia. Electronic address: viktoria.shumyantseva@ibmc.msk.ru.
authors.forename: Victoria V
authors.initials: VV
authors.lastname: Shumyantseva
authors.affiliation: Institute of Biomedical Chemistry, 119121 Moscow, Russia; IBMC-EcoBioPharm Company, 119121 Moscow, Russia.
authors.forename: Tatiana V
authors.initials: TV
authors.lastname: Bulko
authors.affiliation: Institute of Biomedical Chemistry, 119121 Moscow, Russia; Department of Chemistry, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia.
authors.forename: Larisa V
authors.initials: LV
authors.lastname: Sigolaeva
authors.affiliation: Institute of Biomedical Chemistry, 119121 Moscow, Russia; IBMC-EcoBioPharm Company, 119121 Moscow, Russia; N.I. Pirogov Russian National Medical University, 117997 Moscow, Russia.
authors.forename: Alexey V
authors.initials: AV
authors.lastname: Kuzikov
authors.affiliation: Institute of Biomedical Chemistry, 119121 Moscow, Russia; N.I. Pirogov Russian National Medical University, 117997 Moscow, Russia.
authors.forename: Alexander I
authors.initials: AI
authors.lastname: Archakov
mesh_terms: D065966:Absorption, Physicochemical; D003217:Conductometry / Q000295:instrumentation*; D004595:Electroplating / Q000379:methods*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D054802:Molecular Imprinting / Q000379:methods*; D009203:Myocardial Infarction / Q000097:blood* / Q000175:diagnosis; D009211:Myoglobin / Q000097:blood* / Q000737:chemistry; D010655:Phenylenediamines / Q000737:chemistry*; D011485:Protein Binding; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D009211:Myoglobin; D010655:Phenylenediamines; C530487:poly(o-phenylenediamine)
keywords: Electropolymerization; Molecularly imprinted polymers; Myoglobin; Poly-o-phenylenediamine