title: Organic Transistor Arrays Integrated with Finger-Powered Microfluidics for Multianalyte Saliva Testing.
abstract: A compact multianalyte biosensing platform is reported, composed of an organic electrochemical transistor (OECT) microarray integrated with a pumpless "finger-powered" microfluidic, for quantitative screening of glucose, lactate, and cholesterol levels. A biofunctionalization method is designed, which provides selectivity towards specific metabolites as well as minimization of any background interference. In addition, a simple method is developed to facilitate multi-analyte sensing and avoid electrical crosstalk between the different transistors by electrically isolating the individual devices. The resulting biosensing platform, verified using human samples, offers the possibility to be used in easy-to-obtain biofluids with low abundance metabolites, such as saliva. Based on our proposed method, other types of enzymatic biosensors can be integrated into the array to achieve multiplexed, noninvasive, personalized point-of-care diagnostics.
authors.affiliation: Department of Bioelectronics, Ecole Nationale Supérieure des Mines, CMP-EMSE, MOC, 13541, Gardanne, France.
authors.forename: Anna-Maria
authors.initials: AM
authors.lastname: Pappa
authors.affiliation: Department of Bioelectronics, Ecole Nationale Supérieure des Mines, CMP-EMSE, MOC, 13541, Gardanne, France.
authors.forename: Vincenzo F
authors.initials: VF
authors.lastname: Curto
authors.affiliation: Department of Bioelectronics, Ecole Nationale Supérieure des Mines, CMP-EMSE, MOC, 13541, Gardanne, France.
authors.forename: Marcel
authors.initials: M
authors.lastname: Braendlein
authors.affiliation: Department of Bioelectronics, Ecole Nationale Supérieure des Mines, CMP-EMSE, MOC, 13541, Gardanne, France.|Department of Electrical Engineering, University of California, Santa Cruz, CA, 95064, USA.
authors.forename: Xenofon
authors.initials: X
authors.lastname: Strakosas
authors.affiliation: Department of Bioelectronics, Ecole Nationale Supérieure des Mines, CMP-EMSE, MOC, 13541, Gardanne, France.
authors.forename: Mary J
authors.initials: MJ
authors.lastname: Donahue
authors.affiliation: Department of Bioelectronics, Ecole Nationale Supérieure des Mines, CMP-EMSE, MOC, 13541, Gardanne, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Fiocchi
authors.affiliation: Department of Bioelectronics, Ecole Nationale Supérieure des Mines, CMP-EMSE, MOC, 13541, Gardanne, France.
authors.forename: George G
authors.initials: GG
authors.lastname: Malliaras
authors.affiliation: Department of Bioelectronics, Ecole Nationale Supérieure des Mines, CMP-EMSE, MOC, 13541, Gardanne, France. owens@emse.fr.
authors.forename: Roisin M
authors.initials: RM
authors.lastname: Owens
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods*; D005260:Female; D005385:Fingers; D006801:Humans; D056656:Lab-On-A-Chip Devices; D008297:Male; D012463:Saliva / Q000378:metabolism*; D014173:Transistors, Electronic
keywords: microfluidics; multianalyte; organic electrochemical transistors; point-of-care diagnostics; saliva