title: [Studies of bacterial typing with MALDI-TOF].
abstract: MALDI-TOF (matrix-assisted laser desorption ionization time-of-flight) mass spectrometry has emerged as a potential tool for microbial characterization and identification in many microbiology departments. The technology is rapid, sensitive, and relatively inexpensive in terms of both the labour and costs involved. This review provides an overview on its utility for strain typing and epidemiological studies and explains the methodological approaches that can be used both for the performance of the technique and for the analysis of results. Finally, the review summarizes studies on the characterization of distinct bacterial species.
authors.affiliation: Servicio de Microbiología, Hospital Clínico San Carlos, Madrid, España. Electronic address: esther.culebras@salud.madrid.org.
authors.forename: Esther
authors.initials: E
authors.lastname: Culebras
authors.affiliation: Public Health Laboratory, Birmingham, Reino Unido.
authors.forename: Adela
authors.initials: A
authors.lastname: Alvarez-Buylla
authors.affiliation: Servicio de Microbiología, Hospital Universitario Virgen del Rocío, Sevilla, España.
authors.forename: M
authors.initials: M
authors.lastname: Jose Artacho Reinoso
authors.affiliation: Servicio de Microbiología, Hospital Universitario Virgen del Rocío, Sevilla, España.
authors.forename: Jose
authors.initials: J
authors.lastname: Antonio Lepe
mesh_terms: D001419:Bacteria / Q000145:classification / Q000302:isolation & purification; D001424:Bacterial Infections / Q000453:epidemiology / Q000382:microbiology; D015373:Bacterial Typing Techniques / Q000379:methods*; D019295:Computational Biology; D006801:Humans; D013048:Specimen Handling; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
keywords: Bacterial typing; Epidemiologia; Epidemiology; Espectrometria de masas; MALDI-TOF; Mass spectrometry; Tipificacion bacteriana