title: Estimation of the physical properties of neurons and glial cells using dielectrophoresis crossover frequency.
abstract: We successfully determine the ranges of dielectric permittivity, cytoplasm conductivity, and specific membrane capacitance of mouse hippocampal neuronal and glial cells using dielectrophoresis (DEP) crossover frequency (CF). This methodology is based on the simulation of CF directly from the governing equation of a dielectric model of mammalian cells, as well as the measurements of DEP CFs of mammalian cells in different suspension media with different conductivities, based on a simple experimental setup. Relationships between the properties of cells and DEP CF, as demonstrated by theoretical analysis, enable the simultaneous estimation of three properties by a straightforward fitting procedure based on experimentally measured CFs. We verify the effectiveness and accuracy of this approach for primary mouse hippocampal neurons and glial cells, whose dielectric properties, previously, have not been accurately determined. The estimated neuronal properties significantly narrow the value ranges available from the literature. Additionally, the estimated glial cell properties are a valuable addition to the scarce information currently available about this type of cell. This methodology is applicable to any type of cultured cell that can be subjected to both positive and negative dielectrophoresis.
authors.affiliation: Department of Electrical and Computer Engineering, Lehigh University, 16A Memorial Dr. East, Bethlehem, PA, 18015, USA. tiz209@lehigh.edu.
authors.forename: Tianyi
authors.initials: T
authors.lastname: Zhou
authors.affiliation: Department of Electrical and Computer Engineering, Lehigh University, 16A Memorial Dr. East, Bethlehem, PA, 18015, USA.
authors.forename: Yixuan
authors.initials: Y
authors.lastname: Ming
authors.affiliation: Bioengineering Program, Lehigh University, Bethlehem, PA, 18015, USA.|Department of Chemical Engineering, Lehigh University, Bethlehem, PA, 18015, USA.
authors.forename: Susan F
authors.initials: SF
authors.lastname: Perry
authors.affiliation: Department of Electrical and Computer Engineering, Lehigh University, 16A Memorial Dr. East, Bethlehem, PA, 18015, USA. svt2@lehigh.edu.|Bioengineering Program, Lehigh University, Bethlehem, PA, 18015, USA. svt2@lehigh.edu.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Tatic-Lucic
mesh_terms: D000818:Animals; D055592:Biophysical Phenomena; D017097:Electric Impedance; D004586:Electrophoresis; D006624:Hippocampus / Q000166:cytology; D051379:Mice; D009457:Neuroglia / Q000166:cytology*; D009474:Neurons / Q000166:cytology*
keywords: Crossover frequency; Dielectrophoresis (DEP); Electric properties; Fitting procedure; Hippocampal neurons and glial cells