title: Characterization and in vitro evaluation of nimotuzumab conjugated with cisplatin-loaded liposomes.
abstract: In this paper, we report the conjugation of the humanized monoclonal antibody nimotuzumab with cisplatin-loaded liposomes and the in vitro evaluation of its affinity for tumor cells. The conjugation procedure was performed through derivatization of nimotuzumab with N-succinimidyl S-acetylthioacetate (SATA) followed by a covalent attachment with maleimide groups at the end of PEG-DSPE chains located at the membrane of pre-formed liposomes. Confocal microscopy was performed to evaluate the immunoliposome affinity for EGFR antigens from human epidermoid carcinoma (A-431) and normal lung (MRC-5) cell lines. Results showed that the procedures implemented in this work do not affect the capability of the nimotuzumab-immunoliposomes to recognize the tumor cells, which overexpress the EGFR antigens.
authors.affiliation: a Posgrado en Ciencias Químicas , Universidad Nacional Autónoma de México , Ciudad de México , México.
authors.forename: Héctor
authors.initials: H
authors.lastname: Vázquez-Becerra
authors.affiliation: b Subdirección de Investigación Básica , Instituto Nacional de Cancerología , Ciudad de México , México.
authors.forename: Enrique
authors.initials: E
authors.lastname: Pérez-Cárdenas
authors.affiliation: b Subdirección de Investigación Básica , Instituto Nacional de Cancerología , Ciudad de México , México.
authors.forename: Saé
authors.initials: S
authors.lastname: Muñiz-Hernández
authors.affiliation: c Posgrado de Investigación en Medicina , Instituto Politécnico Nacional , Ciudad de México , México.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Izquierdo-Sánchez
authors.affiliation: d Instituto de Física , Universidad Nacional Autónoma de México , Ciudad de México , México , and.|e Unidad de Investigación Biomédica en Cáncer INCan-UNAM, Instituto Nacional de Cancerología , Ciudad de México , México.
authors.forename: Luis Alberto
authors.initials: LA
authors.lastname: Medina
mesh_terms: D061067:Antibodies, Monoclonal, Humanized / Q000737:chemistry* / Q000378:metabolism; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000276:immunology / Q000494:pharmacology; D002294:Carcinoma, Squamous Cell / Q000378:metabolism; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000008:administration & dosage / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D065546:Drug Liberation; D004355:Drug Stability; D049452:Green Fluorescent Proteins / Q000276:immunology; D006801:Humans; D008081:Liposomes / Q000138:chemical synthesis*; D008168:Lung / Q000378:metabolism; D008175:Lung Neoplasms / Q000378:metabolism; D008301:Maleimides / Q000737:chemistry; D010316:Particle Size; D010714:Phosphatidylethanolamines / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D013388:Succinimides / Q000737:chemistry; D013440:Sulfides / Q000737:chemistry; D013499:Surface Properties; D014407:Tumor Cells, Cultured
chemical_list: D061067:Antibodies, Monoclonal, Humanized; D000970:Antineoplastic Agents; D004337:Drug Carriers; D008081:Liposomes; D008301:Maleimides; D010714:Phosphatidylethanolamines; D013388:Succinimides; D013440:Sulfides; C485184:enhanced green fluorescent protein; C107570:polyethylene glycol-distearoylphosphatidylethanolamine; D049452:Green Fluorescent Proteins; C043592:maleimide; D011092:Polyethylene Glycols; C501466:nimotuzumab; C039011:N-hydroxysuccinimide S-acetylthioacetate; D002945:Cisplatin
keywords: Drug carriers; EGFR; immunoliposomes; monoclonal antibody; tumor cell targeting