title: BIRC5 Genomic Copy Number Variation in Early-Onset Breast Cancer.
abstract: BACKGROUND
  Baculoviral inhibitor of apoptosis repeat-containing 5 (BIRC5) gene is an inhibitor of apoptosis that expresses in human embryonic tissues but it is absent in most healthy adult tissues. The copy number of BIRC5 has been indicated to be highly increased in tumor tissues; however, its association with the age of onset in breast cancer is not well understood.
  
  
  METHODS
  Forty tumor tissues of breast cancer were obtained from Tumor Bank of Cancer Institute, Imam Khomeini Hospital, Tehran, Iran. BIRC5 gene copy number variation (CNV) was evaluated using Multiplex Ligation-dependent Probe Amplification (MLPA) and then compared with the age of onset for each patient.
  
  
  RESULTS
  BIRC5 amplification was seen in 17.5% of cases. Also, a significant association was observed between BIRC5 gene amplification and individuals under 40 years of age (P=0.04).
  
  
  CONCLUSIONS
  BIRC5 gene has the potential to be a marker for the detection and prognosis of cancer at an early age.
authors.affiliation: Department of Genetics, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Kimia
authors.initials: K
authors.lastname: Ghaffari
authors.affiliation: Department of Genetics, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Hashemi
authors.affiliation: Group of Genetics, Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Elmira
authors.initials: E
authors.lastname: Ebrahimi
authors.affiliation: Group of Genetics, Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Shirkoohi
mesh_terms: D000328:Adult; D000367:Age Factors; D017668:Age of Onset; D014408:Biomarkers, Tumor / Q000235:genetics*; D001943:Breast Neoplasms / Q000175:diagnosis / Q000235:genetics*; D056915:DNA Copy Number Variations / Q000235:genetics*; D055088:Early Detection of Cancer / Q000379:methods; D005260:Female; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics*; D060885:Multiplex Polymerase Chain Reaction; D000077022:Survivin
chemical_list: C107489:BIRC5 protein, human; D014408:Biomarkers, Tumor; D051034:Inhibitor of Apoptosis Proteins; D000077022:Survivin
keywords: Amplification; BIRC5; Breast cancer; MLPA