title: Dietary arginine silicate inositol complex increased bone healing: histologic and histomorphometric study.
abstract: BACKGROUND
  Arginine silicate inositol complex (ASI; arginine 49.5%, silicon 8.2%, and inositol 25%) is a novel material that is a bioavailable source of silicon and arginine. ASI offers potential benefits for vascular and bone health.
  
  
  OBJECTIVE
  The aim of this study was to evaluate the potential effects of ASI complex on bone healing of critical-sized defects in rats.
  
  
  METHODS
  The rats were randomly assigned to two groups of 21 rats each. The control group was fed a standard diet for 12 weeks; after the first 8 weeks, a calvarial critical-sized defect was created, and the rats were sacrificed 7, 14, and 28 days later. The ASI group was fed a diet containing 1.81 g/kg of ASI for 12 weeks; after the first 8 weeks, a calvarial critical-sized defect was created, and the rats were sacrificed 7, 14, and 28 days later. The calvarial bones of all the rats were then harvested for evaluation.
  
  
  RESULTS
  Osteoblasts and osteoclasts were detected at higher levels in the ASI group compared with the control group at days 7, 14, and 28 of the calvarial defect (P<0.05). New bone formation was detected at higher levels in the ASI group compared with the controls at day 28 (P<0.05). However, new bone formation was not detected at days 7 and 14 in both the groups (P>0.05).
  
  
  CONCLUSIONS
  ASI supplementation significantly improved bone tissue healing in rats with critical-sized defects. This study demonstrated that ASI can enhance bone repair and has potential as a therapeutic regimen in humans.
authors.affiliation: Department of Oral-Maxillofacial Surgery, Faculty of Dentistry, Dicle University, Diyarbakir, Turkey.
authors.forename: Ferhan
authors.initials: F
authors.lastname: Yaman
authors.affiliation: Department of Oral-Maxillofacial Surgery, Faculty of Dentistry, Dicle University, Diyarbakir, Turkey.
authors.forename: Izzet
authors.initials: I
authors.lastname: Acikan
authors.affiliation: Department of Periodontology, Faculty of Dentistry, Firat University, Elazig, Turkey.
authors.forename: Serkan
authors.initials: S
authors.lastname: Dundar
authors.affiliation: Department of Pathology, Faculty of Medicine, Firat University, Elazig, Turkey.
authors.forename: Sercan
authors.initials: S
authors.lastname: Simsek
authors.affiliation: Department of Periodontology, Faculty of Dentistry, Dicle University, Diyarbakir, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Gul
authors.affiliation: Department of Pathology, Faculty of Medicine, Firat University, Elazig, Turkey.
authors.forename: Ibrahim Hanifi
authors.initials: IH
authors.lastname: Ozercan
authors.affiliation: Nutrition 21, LLC, Purchase, NY, USA.
authors.forename: James
authors.initials: J
authors.lastname: Komorowski
authors.affiliation: Department of Animal Nutrition, Faculty of Veterinary Medicine, Firat University, Elazig, Turkey.
authors.forename: Kazim
authors.initials: K
authors.lastname: Sahin
mesh_terms: D000818:Animals; D001120:Arginine / Q000008:administration & dosage / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects*; D019587:Dietary Supplements; D005260:Female; D007294:Inositol / Q000008:administration & dosage / Q000494:pharmacology*; D010006:Osteoblasts / Q000187:drug effects / Q000473:pathology; D010010:Osteoclasts / Q000187:drug effects / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017640:Silicates / Q000008:administration & dosage / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D017640:Silicates; D007294:Inositol; D001120:Arginine
keywords: arginine silicate inositol; bone healing; critical-sized defect; osteoblast; osteoclast