title: EcoTILLING revealed SNPs in GhSus genes that are associated with fiber- and seed-related traits in upland cotton.
abstract: Cotton is the most important textile crop in the world due to its cellulose-enriched fibers. Sucrose synthase genes (Sus) play pivotal roles in cotton fiber and seed development. To mine and pyramid more favorable alleles for cotton molecular breeding, single nucleotide polymorphisms (SNPs) of GhSus family genes were investigated across 277 upland cotton accessions by EcoTILLING. As a result, a total of 24 SNPs in the amplified regions of eight GhSus genes were identified. These SNPs were significantly associated with at least one fiber- or seed-related trait measured in Nanjing, Anyang and Kuche in 2007-2009. Four main-effect quantitative trait nucleotides (QTNs) and five epistatic QTNs, with 0.76-3.56% of phenotypic variances explained by each QTN (PVE), were found to be associated with yield-related traits; six epistatic QTNs, with the 0.43-3.48% PVE, were found to be associated with fiber quality-related traits; and one main-effect QTN and one epistatic QTN, with the PVE of 1.96% and 2.53%, were found to be associated with seed oil content and protein content, respectively. Therefore, this study provides new information for molecular breeding in cotton.
authors.affiliation: State Key Laboratory of Crop Genetics &Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Yan-Da
authors.initials: YD
authors.lastname: Zeng
authors.affiliation: Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China.
authors.forename: Jun-Ling
authors.initials: JL
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Crop Genetics &Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Su-Hong
authors.initials: SH
authors.lastname: Bu
authors.affiliation: State Key Laboratory of Crop Genetics &Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Kang-Sheng
authors.initials: KS
authors.lastname: Deng
authors.affiliation: State Key Laboratory of Crop Genetics &Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Tao
authors.affiliation: College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Yuan-Ming
authors.initials: YM
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Crop Genetics &Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Tian-Zhen
authors.initials: TZ
authors.lastname: Zhang
authors.affiliation: Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China.
authors.forename: Xiong-Ming
authors.initials: XM
authors.lastname: Du
authors.affiliation: State Key Laboratory of Crop Genetics &Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Bao-Liang
authors.initials: BL
authors.lastname: Zhou
mesh_terms: D000483:Alleles; D032561:Cotton Fiber; D018745:Genome, Plant / Q000235:genetics; D003368:Gossypium / Q000235:genetics*; D015810:Linkage Disequilibrium / Q000235:genetics; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D040641:Quantitative Trait Loci / Q000235:genetics*; D012639:Seeds / Q000235:genetics*