title: Simultaneous SPECT imaging of multi-targets to assist in identifying hepatic lesions.
abstract: Molecular imaging technique is an attractive tool to detect liver disease at early stage. This study aims to develop a simultaneous dual-isotope single photon emission computed tomography (SPECT)/CT imaging method to assist diagnosis of hepatic tumor and liver fibrosis. Animal models of liver fibrosis and orthotopic human hepatocellular carcinoma (HCC) were established. The tracers of (131)I-NGA and (99m)Tc-3P-RGD2 were selected to target asialoglycoprotein receptor (ASGPR) on the hepatocytes and integrin αvβ3 receptor in tumor or fibrotic liver, respectively. SPECT imaging and biodistribution study were carried out to verify the feasibility and superiority. As expected, (99m)Tc-3P-RGD2 had the ability to evaluate liver fibrosis and detect tumor lesions. (131)I-NGA showed that it was effective in assessing the anatomy and function of the liver. In synchronized dual-isotope SPECT/CT imaging, clear fusion images can be got within 30 minutes for diagnosing liver fibrosis and liver cancer. This new developed imaging approach enables the acquisition of different physiological information for diagnosing liver fibrosis, liver cancer and evaluating residual functional liver volume simultaneously. So synchronized dual-isotope SPECT/CT imaging with (99m)Tc-3P-RGD2 and (131)I-NGA is an effective approach to detect liver disease, especially liver fibrosis and liver cancer.
authors.affiliation: Center for Molecular Imaging and Translational Medicine, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiang'an South Rd, Xiamen 361102, China.|Department of Isotope, China Institute of Atomic Energy, P. O. Box 2108, Beijing 102413, China.
authors.forename: Zhide
authors.initials: Z
authors.lastname: Guo
authors.affiliation: Center for Molecular Imaging and Translational Medicine, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiang'an South Rd, Xiamen 361102, China.
authors.forename: Mengna
authors.initials: M
authors.lastname: Gao
authors.affiliation: Center for Molecular Imaging and Translational Medicine, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiang'an South Rd, Xiamen 361102, China.
authors.forename: Deliang
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Center for Molecular Imaging and Translational Medicine, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiang'an South Rd, Xiamen 361102, China.|The First Affiliated Hospital of Xiamen University, Zhenhai road, Xiamen 361103, China.
authors.forename: Yesen
authors.initials: Y
authors.lastname: Li
authors.affiliation: Center for Molecular Imaging and Translational Medicine, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiang'an South Rd, Xiamen 361102, China.
authors.forename: Manli
authors.initials: M
authors.lastname: Song
authors.affiliation: Center for Molecular Imaging and Translational Medicine, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiang'an South Rd, Xiamen 361102, China.
authors.forename: Rongqiang
authors.initials: R
authors.lastname: Zhuang
authors.affiliation: Zhongshan Hospital Affiliated of Xiamen University, Hubin South Road, Xiamen 361004, China.
authors.forename: Xinhui
authors.initials: X
authors.lastname: Su
authors.affiliation: The First Affiliated Hospital of Xiamen University, Zhenhai road, Xiamen 361103, China.
authors.forename: Guibing
authors.initials: G
authors.lastname: Chen
authors.affiliation: Center for Molecular Imaging and Translational Medicine, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiang'an South Rd, Xiamen 361102, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Liu
authors.affiliation: Zhongshan Hospital Affiliated of Xiamen University, Hubin South Road, Xiamen 361004, China.
authors.forename: Pingguo
authors.initials: P
authors.lastname: Liu
authors.affiliation: The First Affiliated Hospital of Xiamen University, Zhenhai road, Xiamen 361103, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Isotope, China Institute of Atomic Energy, P. O. Box 2108, Beijing 102413, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Du
authors.affiliation: Center for Molecular Imaging and Translational Medicine, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiang'an South Rd, Xiamen 361102, China.
authors.forename: Xianzhong
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D037263:Asialoglycoprotein Receptor / Q000097:blood; D001345:Autoradiography; D006528:Carcinoma, Hepatocellular / Q000000981:diagnostic imaging*; D005260:Female; D005355:Fibrosis; D022781:Hepatocytes / Q000166:cytology; D006801:Humans; D039302:Integrin alphaVbeta3 / Q000378:metabolism; D007457:Iodine Radioisotopes / Q000737:chemistry; D008113:Liver Neoplasms / Q000000981:diagnostic imaging*; D008279:Magnetic Resonance Imaging; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D009842:Oligopeptides; D015609:Organotechnetium Compounds; D000072078:Positron Emission Tomography Computed Tomography; D000072098:Single Photon Emission Computed Tomography Computed Tomography; D013667:Technetium / Q000737:chemistry; D014018:Tissue Distribution
chemical_list: C546927:ASGR1 protein, human; D037263:Asialoglycoprotein Receptor; D039302:Integrin alphaVbeta3; D007457:Iodine Radioisotopes; D009842:Oligopeptides; D015609:Organotechnetium Compounds; D013667:Technetium; C047981:arginyl-glycyl-aspartic acid