title: MiRNA-203 suppresses cell proliferation, migration and invasion in colorectal cancer via targeting of EIF5A2.
abstract: While it is known that miR-203 is frequently downregulated in many types of human cancer, little is known regarding its expression and functional role in colorectal cancer (CRC). In this study, we aimed to investigate the expression and the potential mechanisms of miR-203 in colorectal cancer. MiR-203 was significantly downregulated in CRC tissues compared with matched normal adjacent tissues. Our clinical data show that decreased miR-203 was associated with an advanced clinical tumor-node-metastasis stage, lymph node metastasis, and poor survival in CRC patients. Furthermore, externally induced expression of miR-203 significantly inhibited CRC cell proliferation and invasion in vitro and in vivo. Mechanistically, we identified EIF5A2 as a direct and functional target of miR-203. The levels of miR-203 were inversely correlated with levels of the EIF5A2 in the CRC tissues. Restoration of EIF5A2 in the miR-203-overexpressing CRC cells reversed the suppressive effects of miR-203. Our results demonstrate that miR-203 serves as a tumor suppressor gene and may be useful as a new potential therapeutic target in CRC.
authors.affiliation: Department of General Surgery, Shanghai General Hospital, Shanghai Jiao Tong University, 100 Haining Road, Shanghai, 200080, China.
authors.forename: Biao
authors.initials: B
authors.lastname: Deng
authors.affiliation: Department of General Surgery, Shanghai General Hospital, Shanghai Jiao Tong University, 100 Haining Road, Shanghai, 200080, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, Tianyou Hospital, TongJi University, 500 Zhennan Road, Shanghai, 200331, China.
authors.forename: Jiaqing
authors.initials: J
authors.lastname: Fang
authors.affiliation: Department of Nuclear Medicine, Shanghai Tenth People's Hospital, TongJi University, 301 Yanchang Road, Shanghai, 200072, China.
authors.forename: Xuchao
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Department of Gastroenterology, Shanghai General Hospital, Shanghai Jiao Tong University, 100 Haining Road, Shanghai, 200080, China.
authors.forename: Zhongwei
authors.initials: Z
authors.lastname: Cao
authors.affiliation: Department of General Surgery, Zhongshan Hospital, Fudan University, 180 Xietu Road, Shanghai, 200032, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Lin
authors.affiliation: Department of General Surgery, Shanghai General Hospital, Shanghai Jiao Tong University, 100 Haining Road, Shanghai, 200080, China.
authors.forename: Lisheng
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of General Surgery, Shanghai General Hospital, Shanghai Jiao Tong University, 100 Haining Road, Shanghai, 200080, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Sun
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000235:genetics*; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D057809:HEK293 Cells; D019073:HT29 Cells; D006801:Humans; D008207:Lymphatic Metastasis / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics*; D010448:Peptide Initiation Factors / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000235:genetics*; D000097574:Eukaryotic Translation Initiation Factor 5A
chemical_list: D035683:MicroRNAs; D010448:Peptide Initiation Factors; D016601:RNA-Binding Proteins; D000097574:Eukaryotic Translation Initiation Factor 5A; C546703:MIRN203 microRNA, human