title: Portable, Constriction-Expansion Blood Plasma Separation and Polymerization-Based Malaria Detection.
abstract: A portable, microfluidic blood plasma separation device is presented featuring a constriction-expansion design, which produces 100.0% purity for undiluted blood at 9% yield. This level of purity represents an improvement of at least 1 order of magnitude with increased yield compared to that achieved previously using passive separation. The system features high flow rates, 5-30 μL/min plasma collection, with minimal clogging and biofouling. The simple, portable blood plasma separation design is hand-driven and can easily be incorporated with microfluidic or laboratory scale diagnostic assays. The separation system was applied to a paper-based diagnostic test for malaria that produced an amplified color change in the presence of Plasmodium falciparum histidine-rich protein 2 at a concentration well below clinical relevancy for undiluted whole blood.
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology , 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States.
authors.forename: Tatyana A
authors.initials: TA
authors.lastname: Shatova
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology , 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States.
authors.forename: Shefali
authors.initials: S
authors.lastname: Lathwal
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology , 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States.
authors.forename: Marissa R
authors.initials: MR
authors.lastname: Engle
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology , 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States.
authors.forename: Hadley D
authors.initials: HD
authors.lastname: Sikes
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology , 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States.
authors.forename: Klavs F
authors.initials: KF
authors.lastname: Jensen
mesh_terms: D000913:Antibodies, Protozoan / Q000276:immunology; D000953:Antigens, Protozoan / Q000097:blood* / Q000276:immunology; D003124:Colorimetry; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry; D057230:Limit of Detection; D008288:Malaria / Q000175:diagnosis* / Q000469:parasitology; D044085:Microfluidics / Q000379:methods*; D010963:Plasmodium falciparum / Q000302:isolation & purification / Q000378:metabolism; D019095:Point-of-Care Systems; D015800:Protozoan Proteins / Q000097:blood* / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; C514457:HRP-2 antigen, Plasmodium falciparum; D015800:Protozoan Proteins; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate