title: Novel Monoclonal Antibodies for Studies of Human and Rhesus Macaque Secretory Component and Human J-Chain.
abstract: Immunoglobulin A (IgA) antibodies exist in monomeric, dimeric, and secretory forms. Dimerization of IgA depends on a 15-kD polypeptide termed "joining (J) chain," which is also part of the binding site for an epithelial glycoprotein called "secretory component (SC)," whether this after apical cleavage on secretory epithelia is ligand bound in secretory IgA (SIgA) or in a free form. Uncleaved membrane SC, also called the "polymeric Ig receptor," is thus crucial for transcytotic export of SIgA to mucosal surfaces, where it interacts with and modulates commensal bacteria and mediates protective immune responses against exogenous pathogens. To evaluate different forms of IgA, we have produced mouse monoclonal antibodies (MAbs) against human J-chain and free SC. We found that J-chain MAb 9A8 and SC MAb 9H7 identified human dimeric IgA and SIgA in enzyme-linked immunoassay and western blot analysis, as well as functioning in immunohistochemistry to identify cytoplasmic IgA of intestinal lamina propria plasmablasts/plasma cells and crypt epithelium of distal human intestine. Finally, we demonstrated that SC MAb 9H7 cross-reacted with rhesus macaque SIgA. These novel reagents should be of use in the study of the biology of various forms of IgA in humans and SIgA in macaques, as well as in monitoring the production and/or isolation of these forms of IgA.
authors.affiliation: 1 Duke Human Vaccine Institute, Duke University School of Medicine , Durham, North Carolina.
authors.forename: Ruijun
authors.initials: R
authors.lastname: Zhang
authors.affiliation: 1 Duke Human Vaccine Institute, Duke University School of Medicine , Durham, North Carolina.|2 Department of Medicine, Duke University School of Medicine , Durham, North Carolina.
authors.forename: S Munir
authors.initials: SM
authors.lastname: Alam
authors.affiliation: 1 Duke Human Vaccine Institute, Duke University School of Medicine , Durham, North Carolina.
authors.forename: Jae-Sung
authors.initials: JS
authors.lastname: Yu
authors.affiliation: 1 Duke Human Vaccine Institute, Duke University School of Medicine , Durham, North Carolina.
authors.forename: Richard
authors.initials: R
authors.lastname: Scearce
authors.affiliation: 1 Duke Human Vaccine Institute, Duke University School of Medicine , Durham, North Carolina.
authors.forename: Bradley
authors.initials: B
authors.lastname: Lockwood
authors.affiliation: 1 Duke Human Vaccine Institute, Duke University School of Medicine , Durham, North Carolina.
authors.forename: Kwan-Ki
authors.initials: KK
authors.lastname: Hwang
authors.affiliation: 1 Duke Human Vaccine Institute, Duke University School of Medicine , Durham, North Carolina.
authors.forename: Robert
authors.initials: R
authors.lastname: Parks
authors.affiliation: 1 Duke Human Vaccine Institute, Duke University School of Medicine , Durham, North Carolina.|3 Department of Pediatrics, Duke University School of Medicine , Durham, North Carolina.
authors.forename: Sallie
authors.initials: S
authors.lastname: Permar
authors.affiliation: 4 Centre for Immune Regulation (CIR), University of Oslo , Oslo, Norway .|5 LIIPAT, Department Pathology, Oslo University Hospital Rikshospitalet , Oslo, Norway .
authors.forename: Per
authors.initials: P
authors.lastname: Brandtzaeg
authors.affiliation: 1 Duke Human Vaccine Institute, Duke University School of Medicine , Durham, North Carolina.|2 Department of Medicine, Duke University School of Medicine , Durham, North Carolina.
authors.forename: Barton F
authors.initials: BF
authors.lastname: Haynes
authors.affiliation: 1 Duke Human Vaccine Institute, Duke University School of Medicine , Durham, North Carolina.|2 Department of Medicine, Duke University School of Medicine , Durham, North Carolina.
authors.forename: Hua-Xin
authors.initials: HX
authors.lastname: Liao
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D003429:Cross Reactions / Q000276:immunology; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000276:immunology*; D007133:Immunoglobulin Joining Region / Q000276:immunology*; D008253:Macaca mulatta / Q000276:immunology; D051379:Mice
chemical_list: D000911:Antibodies, Monoclonal; D007071:Immunoglobulin A, Secretory; D007133:Immunoglobulin Joining Region