title: The Effects of Salicylic Acid on Juvenile Zebrafish Danio rerio Under Flow-Through Conditions.
abstract: The aquatic environment is becoming increasingly contaminated with pharmaceuticals. Salicylic acid (SA), which can be used individually or appear as a degradation product of the widely used acetylsalicylic acid was chosen for testing. Juvenile zebrafish Danio rerio were subjected to OECD test No. 215 (fish, juvenile growth test) with salicylic acid concentrations of 0.004; 0.04; 0.4; 4 and 40 mg/L. Specific growth rate (SGR), histological changes, and parameters of oxidative stress were evaluated. SA had no effects on histological changes, SGR, glutathione reductase, and lipid peroxidation. Increased catalytic activity of GPx was found at 0.04 mg/L compared to control, increased catalytic activity of catalase was found at 0.04 and 4 mg/L compared to control, and increased catalytic activity of glutathione-S-transferase was found at 0.004 and 0.04 mg/L compared to control (P < 0.05). Juvenile zebrafish turned out to be relatively insensitive to both environmentally relevant (0.004 mg/L) and higher concentrations of salicylic acid.
authors.affiliation: Department of Animal Protection, Welfare and Behaviour, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho tr. 1946/1, 612 42, Brno, Czech Republic.
authors.forename: Dana
authors.initials: D
authors.lastname: Zivna
authors.affiliation: Department of Animal Protection, Welfare and Behaviour, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho tr. 1946/1, 612 42, Brno, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Blahova
authors.affiliation: Department of Animal Protection, Welfare and Behaviour, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho tr. 1946/1, 612 42, Brno, Czech Republic. sirokaz@vfu.cz.
authors.forename: Zuzana
authors.identifier: http://orcid.org/0000-0002-8283-1179
authors.initials: Z
authors.lastname: Siroka
authors.affiliation: Department of Animal Protection, Welfare and Behaviour, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho tr. 1946/1, 612 42, Brno, Czech Republic.
authors.forename: Lucie
authors.initials: L
authors.lastname: Plhalova
authors.affiliation: Department of Animal Protection, Welfare and Behaviour, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho tr. 1946/1, 612 42, Brno, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Marsalek
authors.affiliation: Department of Animal Protection, Welfare and Behaviour, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho tr. 1946/1, 612 42, Brno, Czech Republic.
authors.forename: Veronika
authors.initials: V
authors.lastname: Doubkova
authors.affiliation: Department of Veterinary Public Health and Forensic Medicine, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho tr. 1946/1, 612 42, Brno, Czech Republic.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Zelinska
authors.affiliation: Department of Animal Protection, Welfare and Behaviour, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho tr. 1946/1, 612 42, Brno, Czech Republic.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Vecerek
authors.affiliation: Department of Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho tr. 1946/1, 612 42, Brno, Czech Republic.
authors.forename: Frantisek
authors.initials: F
authors.lastname: Tichy
authors.affiliation: Department of Animal Protection, Welfare and Behaviour, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho tr. 1946/1, 612 42, Brno, Czech Republic.
authors.forename: Pavla
authors.initials: P
authors.lastname: Sehonova
authors.affiliation: Department of Animal Protection, Welfare and Behaviour, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho tr. 1946/1, 612 42, Brno, Czech Republic.
authors.forename: Zdenka
authors.initials: Z
authors.lastname: Svobodova
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D018384:Oxidative Stress; D020156:Salicylic Acid / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*; D015027:Zebrafish / Q000378:metabolism / Q000502:physiology*
chemical_list: D014874:Water Pollutants, Chemical; D002374:Catalase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D020156:Salicylic Acid
keywords: NSAIDs; Oxidative stress; Pharmaceuticals; Specific growth rate; Water contamination