title: Maternal gene-micronutrient interactions related to one-carbon metabolism and the risk of myelomeningocele among offspring.
abstract: Few studies have evaluated interactions between maternal genetic variation in 5,10-methylenetetrahydrofolate reductase (MTHFR) and micronutrient intake on the risk of myelomeningocele (MM) in offspring. Therefore, we sought to determine if the role of maternal MTHFR C677T and A1298C on MM risk is altered by maternal intake of micronutrients related to one-carbon metabolism.
  
  
  
  The study consisted of 220 MM case-parent trios recruited from 1996 to 2006. A dietary questionnaire was used to obtain information on maternal dietary intake on eight micronutrients including folate and cobalamin. TaqMan assays were used to generate MTHFR C677T and A1298C genotypes. Log-linear models were used to evaluate the joint effects of maternal genotype and micronutrient intake dichotomized as at or above versus below the United States Recommended Dietary Allowance (US RDA) on MM.
  
  
  
  There was little evidence to suggest maternal MTHFR genotypes interacted with micronutrient intake to influence the risk of MM. For instance, the effect of MTHFR 677T was similar for mothers with cobalamin intake below US RDA (relative risk [RR] = 0.97) versus at or above US RDA cobalamin intake (RR = 0.81, interaction p = 0.87). However, some differences were noted. For example, the effect of MTHFR 1298C appeared to be different between those mothers below US RDA folate intake (RR = 0.98) versus those at or above US RDA folate intake (RR = 0.68), but the interaction was not statistically significant (interaction p = 0.27).
  
  
  
  There did not appear to be strong effects of maternal micronutrient intake on the role of maternal genetic polymorphisms in MTHFR C677T and A1298C on MM risk. Birth Defects Research 109:99-105, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Divisions of Neonatology, Department of Pediatrics, University of Texas Health Science Center at Houston McGovern Medical School, Houston, Texas.
authors.forename: Margaret P
authors.identifier: 0000-0001-6968-9457
authors.initials: MP
authors.lastname: Nguyen
authors.affiliation: Section of Hematology-Oncology, Department of Pediatrics, Baylor College of Medicine, Houston, Texas.
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Lupo
authors.affiliation: Division of Medical Genetics, Department of Pediatrics, University of Texas Health Science Center at Houston McGovern Medical School, Houston, Texas.
authors.forename: Hope
authors.initials: H
authors.lastname: Northrup
authors.affiliation: Department of Epidemiology, Human Genetics & Environmental Sciences, University of Texas Health Science Center at Houston, School of Public Health, Houston, Texas.
authors.forename: Alanna C
authors.initials: AC
authors.lastname: Morrison
authors.affiliation: Department of Psychology, University of Houston, Houston, Texas.
authors.forename: Paul T
authors.initials: PT
authors.lastname: Cirino
authors.affiliation: Division of Medical Genetics, Department of Pediatrics, University of Texas Health Science Center at Houston McGovern Medical School, Houston, Texas.
authors.forename: Kit Sing
authors.initials: KS
authors.lastname: Au
mesh_terms: D000483:Alleles; D015930:Diet Records; D005260:Female; D005492:Folic Acid / Q000008:administration & dosage*; D015870:Gene Expression; D059647:Gene-Environment Interaction; D005838:Genotype; D006801:Humans; D007231:Infant, Newborn; D008591:Meningomyelocele / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics* / Q000378:metabolism; D018977:Micronutrients / Q000008:administration & dosage*; D020641:Polymorphism, Single Nucleotide; D064167:Recommended Dietary Allowances; D012306:Risk; D011795:Surveys and Questionnaires; D014481:United States; D014805:Vitamin B 12 / Q000008:administration & dosage*
chemical_list: D018977:Micronutrients; D005492:Folic Acid; C573423:MTHFR protein, human; D042965:Methylenetetrahydrofolate Reductase (NADPH2); D014805:Vitamin B 12
keywords: cobalamin; folate; myelomeningocele; niacin; pyridoxine; riboflavin; thiamin