title: Envelope-specific B-cell populations in African green monkeys chronically infected with simian immunodeficiency virus.
abstract: African green monkeys (AGMs) are natural primate hosts of simian immunodeficiency virus (SIV). Interestingly, features of the envelope-specific antibody responses in SIV-infected AGMs are distinct from that of HIV-infected humans and SIV-infected rhesus monkeys, including gp120-focused responses and rapid development of autologous neutralization. Yet, the lack of genetic tools to evaluate B-cell lineages hinders potential use of this unique non-human primate model for HIV vaccine development. Here we define features of the AGM Ig loci and compare the proportion of Env-specific memory B-cell populations to that of HIV-infected humans and SIV-infected rhesus monkeys. AGMs appear to have a higher proportion of Env-specific memory B cells that are mainly gp120 directed. Furthermore, AGM gp120-specific monoclonal antibodies display robust antibody-dependent cellular cytotoxicity and CD4-dependent virion capture activity. Our results support the use of AGMs to model induction of functional gp120-specific antibodies by HIV vaccine strategies.
authors.affiliation: Duke Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina 27710, USA.
authors.forename: Ruijun
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Duke Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina 27710, USA.
authors.forename: David R
authors.initials: DR
authors.lastname: Martinez
authors.affiliation: Duke Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina 27710, USA.
authors.forename: Quang N
authors.initials: QN
authors.lastname: Nguyen
authors.affiliation: Duke Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina 27710, USA.|Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710, USA.
authors.forename: Justin
authors.initials: J
authors.lastname: Pollara
authors.affiliation: Duke Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina 27710, USA.
authors.forename: Trina
authors.initials: T
authors.lastname: Arifin
authors.affiliation: Duke Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina 27710, USA.
authors.forename: Christina
authors.initials: C
authors.lastname: Stolarchuk
authors.affiliation: Duke Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina 27710, USA.
authors.forename: Andrew
authors.initials: A
authors.lastname: Foulger
authors.affiliation: Duke Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina 27710, USA.
authors.forename: Josh D
authors.initials: JD
authors.lastname: Amos
authors.affiliation: Duke Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina 27710, USA.
authors.forename: Robert
authors.initials: R
authors.lastname: Parks
authors.affiliation: Duke Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina 27710, USA.
authors.forename: Jonathon E
authors.initials: JE
authors.lastname: Himes
authors.affiliation: Duke Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina 27710, USA.
authors.forename: Minyue
authors.initials: M
authors.lastname: Wang
authors.affiliation: Duke Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina 27710, USA.
authors.forename: Regina W
authors.initials: RW
authors.lastname: Edwards
authors.affiliation: Duke Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina 27710, USA.
authors.forename: Ashley M
authors.initials: AM
authors.lastname: Trama
authors.affiliation: Duke Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina 27710, USA.
authors.forename: Nathan
authors.initials: N
authors.lastname: Vandergrift
authors.affiliation: Duke Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina 27710, USA.
authors.forename: Lisa
authors.initials: L
authors.lastname: Colvin
authors.affiliation: Research Institute of the McGill University Health Centre, McGill University and Génome Québec Innovation Centre, Montreal, Québec, Canada H3A 0G1.|Department of Human Genetics, McGill University, Montreal, Québec, Canada H3A 1B1.|Department of Experimental Medicine, McGill University, Montreal, Québec, Canada H3A 1A3.
authors.forename: Ken
authors.initials: K
authors.lastname: Dewar
authors.affiliation: Research Institute of the McGill University Health Centre, McGill University and Génome Québec Innovation Centre, Montreal, Québec, Canada H3A 0G1.|Department of Human Genetics, McGill University, Montreal, Québec, Canada H3A 1B1.|Department of Experimental Medicine, McGill University, Montreal, Québec, Canada H3A 1A3.
authors.forename: Nikoleta
authors.initials: N
authors.lastname: Juretic
authors.affiliation: Research Institute of the McGill University Health Centre, McGill University and Génome Québec Innovation Centre, Montreal, Québec, Canada H3A 0G1.|Department of Human Genetics, McGill University, Montreal, Québec, Canada H3A 1B1.|Department of Experimental Medicine, McGill University, Montreal, Québec, Canada H3A 1A3.
authors.forename: Jessica
authors.initials: J
authors.lastname: Wasserscheid
authors.affiliation: Duke Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina 27710, USA.|Department of Surgery, Duke University Medical Center, Durham, North Carolina 27710, USA.
authors.forename: Guido
authors.initials: G
authors.lastname: Ferrari
authors.affiliation: Duke Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina 27710, USA.
authors.forename: Hua-Xin
authors.initials: HX
authors.lastname: Liao
authors.affiliation: Duke Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina 27710, USA.|Department of Pediatrics, Duke University Medical Center, Durham, North Carolina 27705, USA.
authors.forename: Sallie R
authors.initials: SR
authors.lastname: Permar
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000096:biosynthesis* / Q000737:chemistry; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000737:chemistry; D001402:B-Lymphocytes / Q000276:immunology* / Q000821:virology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000821:virology; D002522:Chlorocebus aethiops; D002908:Chronic Disease; D003602:Cytotoxicity, Immunologic; D014644:Genetic Variation; D015699:HIV Envelope Protein gp120 / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular; D007136:Immunoglobulins / Q000096:biosynthesis* / Q000145:classification; D007156:Immunologic Memory; D008253:Macaca mulatta; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000821:virology; D015302:Simian Immunodeficiency Virus / Q000276:immunology* / Q000472:pathogenicity; D014771:Virion / Q000276:immunology / Q000472:pathogenicity
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D015699:HIV Envelope Protein gp120; D007136:Immunoglobulins