title: [Tripartite motif-containing protein 34 (TRIM34) colocalized with micronuclei chromosome and hampers its movement to equatorial plate during the metaphase stage of mitosis].
abstract: Objective To examine whether tripartite motif-containing protein 34 (TRIM34) is colocalized with micronuclei and investigate the influence on the movement of micronuclei chromosome in mitosis. Methods The eukaryotic expression vector TRIM34-pEGFP-N3 was constructed, identified and then transfected into HEK293T cells. With 4', 6-diamidino-2-phenylindole 2HCI (DAPI) staining, the colocalization between TRIM34 and micronuclei was observed under a fluorescence microscope. Moreover, MitoTracker(R)Deep Red was used to identify the colocalization between the complex of TRIM34-micronulei and mitochondria under a confocal microscope. Finally, the effect of TRIM34 on the movement of micronuclei chromosome in mitosis was examined. Results DNA sequencing confirmed that the vector TRIM34-pEGFP-N3 was constructed successfully. A fluorescence microscope revealed that TRIM34 could be colocalized with micronuclei in HEK293T cells transfected with TRIM34-pEGFP-N3. In the same manner, a confocal microscope distinctly showed that TRIM34 was colocalized with micronuclei similarly in appearance. However, there was no distinguished colocalization relationship between the complex of TRIM34-micronulei and mitochondria. Interestingly, the micronuclei chromosome conjugated with TRIM34 was hardly transferred to equatorial plate during the metaphase stage of mitosis. Conclusion TRIM34 is colocalized with micronuclei chromosome and hampers its movement to equatorial plate in mitosis.
authors.affiliation: Laboratory of Clinical Medicine, Hospital Affiliated to Binzhou Medical University, Binzhou 256603, China. *Corresponding author, E-mail: sdkaaa@163.com.
authors.forename: Dakang
authors.initials: D
authors.lastname: Sun
authors.affiliation: Department of Clinical Laboratory, Hospital Affiliated to Binzhou Medical University, Binzhou 256603, China.
authors.forename: Xinye
authors.initials: X
authors.lastname: An
authors.affiliation: Department of Clinical Laboratory, Hospital Affiliated to Binzhou Medical University, Binzhou 256603, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Ji
authors.affiliation: Department of Cardiovascular Diseases, Hospital Affiliated to Binzhou Medical University, Binzhou 256603, China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Laboratory of Clinical Medicine, Hospital Affiliated to Binzhou Medical University, Binzhou 256603, China.
authors.forename: Honglian
authors.initials: H
authors.lastname: Gao
authors.affiliation: Laboratory of Clinical Medicine, Hospital Affiliated to Binzhou Medical University, Binzhou 256603, China.
authors.forename: Mingming
authors.initials: M
authors.lastname: Tian
mesh_terms: D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020090:Chromosome Segregation / Q000235:genetics / Q000502:physiology*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D007211:Indoles / Q000737:chemistry; D008677:Metaphase / Q000235:genetics; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000737:chemistry / Q000378:metabolism; D008938:Mitosis / Q000235:genetics / Q000502:physiology*; D008954:Models, Biological; D009930:Organic Chemicals / Q000737:chemistry; D011485:Protein Binding; D014162:Transfection
chemical_list: D002352:Carrier Proteins; D007211:Indoles; C507919:MitoTracker Red 580; D009930:Organic Chemicals; C417918:TRIM34 protein, human; D049452:Green Fluorescent Proteins; C007293:DAPI