title: Conservation, Innovation, and Bias: Embryonic Segment Boundaries Position Posterior, but Not Anterior, Head Horns in Adult Beetles.
abstract: The integration of form and function of novel traits is a fundamental process during the developmental evolution of complex organisms, yet how novel traits and trait functions integrate into preexisting contexts remains poorly understood. Here, we explore the mechanisms by which the adult insect head has been able to integrate novel traits and features during its ontogeny, focusing on the cephalic horns of Onthophagus beetles. Specifically, using a microablation approach we investigate how different regions of the dorsal head of adult horned beetles relate to their larval and embryonic counterparts and test whether deeply conserved regional boundaries that establish the embryonic head might also facilitate or bias the positioning of cephalic horns along the dorsal adult head. We find that paired posterior horns-the most widespread horn type within the genus-are positioned along a border homologous to the embryonic clypeolabral (CL)-ocular boundary, and that this placement constitutes the ancestral form of horn positioning. In contrast, we observed that the phylogenetically much rarer anterior horns are positioned by larval head regions contained firmly within the CL segment and away from any major preexisting larval head landmarks or boundaries. Lastly, we describe the unexpected finding that ablations at medial head regions can result in ectopic outgrowths bearing terminal structures resembling the more anterior clypeal ridge. We discuss our results in the light of the developmental genetic mechanisms of head formation in holometabolous insects and the role of co-option in innovation and bias in developmental evolution.
authors.affiliation: Department of Biology, Indiana University, Bloomington, Indiana.
authors.forename: Hannah A
authors.initials: HA
authors.lastname: Busey
authors.affiliation: Department of Biology, Indiana University, Bloomington, Indiana.
authors.forename: Eduardo E
authors.initials: EE
authors.lastname: Zattara
authors.affiliation: Department of Biology, Indiana University, Bloomington, Indiana.
authors.forename: Armin P
authors.initials: AP
authors.lastname: Moczek
mesh_terms: D000818:Animals; D005075:Biological Evolution; D001517:Coleoptera / Q000033:anatomy & histology* / Q000196:embryology / Q000254:growth & development; D004625:Embryo, Nonmammalian / Q000033:anatomy & histology / Q000196:embryology; D005260:Female; D006257:Head / Q000033:anatomy & histology / Q000196:embryology / Q000254:growth & development; D007814:Larva / Q000033:anatomy & histology / Q000254:growth & development; D008297:Male