title: Polypeptides from the Skin of Rana chensinensis Exert the Antioxidant and Antiapoptotic Activities on HaCaT Cells.
abstract: Studies have shown that frog skin secretes many types of peptides that are good for human skin. In this study, acid and enzymatic extracts of Rana skin peptides (acid/enzymatic Rana skin peptides, ARPs/ERPs) were obtained. The chemical and physical properties of the ARPs and ERPs were identified through UV scanning, HGLC, FRIT, and MS. MTS and flow cytometry were used to test the proproliferative and antiapoptotic effects of the ARPs and ERPs on human immortalized keratinocytes (HaCaT cells). To elucidate the antiapoptotic mechanisms, the mRNA and protein levels of EGF (epidermal growth factor, which enhances stimulation of cellular proliferation in both cells and epithelial tissues) and caspase-3 were evaluated using quantitative RT-PCR. The results indicated that the ARPs and ERPs were extracted from the Rana skin with yields of 0.65% and 0.52%, respectively. Treatment with ARPs (1.6 g/L) and ERPs (0.8 g/L) showed a 1.66-fold (p < 0.001) and 2.1-fold (p < 0.001) enhancement in the proliferation rates of HaCaT cells. The rate of apoptosis decreased by 2.6 fold (p < 0.01) and 3.4 fold (p < 0.01) under the UVB stimulation, respectively, at the same time, the up-regulation of EGF and down-regulation of caspase-3 were found. These results suggested that we can dig into the potential value of ARPs/ERPs in a new field.
authors.affiliation: a College of Animal Science , Jilin University , Changchun , Jilin Province , China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: a College of Animal Science , Jilin University , Changchun , Jilin Province , China.
authors.forename: Yunyun
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: b Institute of Translational Medicine , The First Hospital, Jilin University , Changchun , Jilin Province , China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: a College of Animal Science , Jilin University , Changchun , Jilin Province , China.
authors.forename: Songcai
authors.initials: S
authors.lastname: Liu
authors.affiliation: a College of Animal Science , Jilin University , Changchun , Jilin Province , China.
authors.forename: Hui
authors.initials: H
authors.lastname: Shi
authors.affiliation: a College of Animal Science , Jilin University , Changchun , Jilin Province , China.
authors.forename: Chao
authors.initials: C
authors.lastname: Lu
authors.affiliation: a College of Animal Science , Jilin University , Changchun , Jilin Province , China.
authors.forename: Siming
authors.initials: S
authors.lastname: Li
authors.affiliation: a College of Animal Science , Jilin University , Changchun , Jilin Province , China.
authors.forename: Linyan
authors.initials: L
authors.lastname: Nie
authors.affiliation: a College of Animal Science , Jilin University , Changchun , Jilin Province , China.
authors.forename: Dan
authors.initials: D
authors.lastname: Su
authors.affiliation: a College of Animal Science , Jilin University , Changchun , Jilin Province , China.
authors.forename: Xuming
authors.initials: X
authors.lastname: Deng
authors.affiliation: c Department of Radiation Medicine, School of Public Health and Tropic Medicine , Southern Medical University , Guangzhou , Guangzhou Province , China.
authors.forename: Kexiang
authors.initials: K
authors.lastname: Ding
authors.affiliation: a College of Animal Science , Jilin University , Changchun , Jilin Province , China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Hao
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects; D053148:Caspase 3 / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D015536:Down-Regulation / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D011898:Ranidae / Q000378:metabolism*; D012867:Skin / Q000737:chemistry* / Q000378:metabolism; D014466:Ultraviolet Rays; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000975:Antioxidants; D010455:Peptides; D053148:Caspase 3
keywords: Apoptosis; HaCaT cells; Polypeptides; Rana skin