title: Preparation and Biochemical Analysis of Classical Histone Deacetylases.
abstract: Histone deacetylase assays were first developed in the 1970s, and subsequently refined in the 1990s with the cloning of HDAC enzymes. Most of these early assays, relying on traditional in vitro chemical methodologies, are still applicable today. More recently, however, cell-based HDAC assays that measure HDAC activities in physiological conditions are emerging. Also, there is a continuing development of assays that can measure an isolated HDAC in the absence of other HDAC activities. This chapter reviews some of the older established methods for assaying HDAC activities, as well as introduces more recently developed nontraditional assays.
authors.affiliation: George Washington University Cancer Center, School of Medicine and Health Sciences, The George Washington University, Washington, DC, United States.
authors.forename: A
authors.initials: A
authors.lastname: Villagra
authors.affiliation: H. Lee Moffitt Cancer Center, Tampa, FL, United States.
authors.forename: E
authors.initials: E
authors.lastname: Sahakian
authors.affiliation: George Washington University Cancer Center, School of Medicine and Health Sciences, The George Washington University, Washington, DC, United States. Electronic address: seto@gwu.edu.
authors.forename: E
authors.initials: E
authors.lastname: Seto
mesh_terms: D000818:Animals; D047369:Chromatin Immunoprecipitation / Q000379:methods; D004353:Drug Evaluation, Preclinical / Q000379:methods; D057075:Enzyme Assays / Q000379:methods*; D055786:Gene Knockout Techniques / Q000379:methods; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology*; D006655:Histone Deacetylases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D013379:Substrate Specificity
chemical_list: D056572:Histone Deacetylase Inhibitors; D006655:Histone Deacetylases
keywords: HDAC; HDAC assay; HDAC inhibitor; HDAC substrate; Histone deacetylase