title: Specific Detection of Dog Podoplanin Expressed in Renal Glomerulus by a Novel Monoclonal Antibody PMab-38 in Immunohistochemistry.
abstract: Podoplanin (PDPN) is expressed in several normal tissues including podocytes of renal glomerulus, lymphatic endothelial cells (LECs), and type I alveolar cells of lung. PDPN activates platelet aggregation by binding to C-type lectin-like receptor-2 (CLEC-2) on platelets. Many monoclonal antibodies (mAbs) against human PDPN, mouse PDPN, rat PDPN, rabbit PDPN, and bovine PDPN have been established; antidog PDPN (dPDPN) mAbs have not been developed. Herein, we immunized mice with the recombinant proteins of dPDPN and developed anti-dPDPN mAbs. One of the clones, PMab-38, is useful for detecting podocytes in immunohistochemical analysis; in contrast, it did not react with LECs or type I alveolar cells. PMab-38 also detected dPDPN specifically in flow cytometry and Western blot analysis. PMab-38 is expected to be useful for investigating the function of dPDPN, which is expressed in podocytes.
authors.affiliation: 1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .|2 Department of Orthopaedic Surgery, Yamagata University Faculty of Medicine , Yamagata, Japan .
authors.forename: Ryusuke
authors.initials: R
authors.lastname: Honma
authors.affiliation: 1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .
authors.forename: Mika K
authors.initials: MK
authors.lastname: Kaneko
authors.affiliation: 1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ogasawara
authors.affiliation: 1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .
authors.forename: Yuki
authors.initials: Y
authors.lastname: Fujii
authors.affiliation: 3 Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University , Sapporo, Japan .
authors.forename: Satoru
authors.initials: S
authors.lastname: Konnai
authors.affiliation: 2 Department of Orthopaedic Surgery, Yamagata University Faculty of Medicine , Yamagata, Japan .
authors.forename: Michiaki
authors.initials: M
authors.lastname: Takagi
authors.affiliation: 1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Sendai, Japan .
authors.forename: Yukinari
authors.initials: Y
authors.lastname: Kato
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology*; D002417:Cattle; D006224:Cricetinae; D004285:Dogs; D005786:Gene Expression Regulation / Q000276:immunology; D006801:Humans; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification; D050527:Mesangial Cells / Q000276:immunology / Q000378:metabolism; D051379:Mice; D010974:Platelet Aggregation / Q000276:immunology; D050199:Podocytes / Q000276:immunology; D011817:Rabbits; D051381:Rats; D000098853:Podoplanin
chemical_list: D000911:Antibodies, Monoclonal; D008562:Membrane Glycoproteins; C117468:PDPN protein, human; D000098853:Podoplanin