title: Paenibacillus silagei sp. nov. isolated from corn silage.
abstract: A Gram-stain-negative, rod-shaped, endospore-forming bacterium, strain LOOC204T, was isolated from corn silage that had been prepared and stored in Hokkaido, Japan. The isolate grew well at 4-37 °C and at pH 6.0-8.5. The major cellular fatty acids were anteiso-C15 : 0, C16 : 0, iso-C14 : 0 and iso-C16 : 0. The major menaquinone was MK-7. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, three unidentified aminophospholipids, one unidentified phospholipid, two unidentified aminolipids and three unidentified lipids were detected as the cellular polar lipids. The genomic DNA G+C content of LOOC204T was 52.2±0.7 mol%. A comparative 16S rRNA gene sequence analysis showed that LOOC204T represented a member of the genus Paenibacillus, and high levels of sequence similarity of LOOC204T with Paenibacillus borealis KK19T (97.5 %) and Paenibacillusjilunlii Be17T (97.4 %) were found. Based on sequence analysis of the RNA polymerase β subunit (rpoB) gene, LOOC204T was clearly separated from its phylogenetic neighbors in the genus Paenibacillus. DNA-DNA hybridisation experiments showed that LOOC204T displayed less than 40 % reassociation values toward its nearest phylogenetic neighbours of the genus Paenibacillus, indicating the unique taxonomic status of LOOC204T. Based on these data, we conclude that strain LOOC204T represents a novel species of the genus Paenibacillus, named Paenibacillus silageisp. nov. The type strain is LOOC204T (=JCM 30974T=DSM 101953T).
authors.affiliation: Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization, Nasushiobara 329-2793, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Tohno
authors.affiliation: Japan Collection of Microorganisms, RIKEN BioResource Center, Tsukuba, Ibaraki 305-0074, Japan.
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Sakamoto
authors.affiliation: Japan Collection of Microorganisms, RIKEN BioResource Center, Tsukuba, Ibaraki 305-0074, Japan.
authors.forename: Moriya
authors.initials: M
authors.lastname: Ohkuma
authors.affiliation: Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305-0901, Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Tajima
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D007564:Japan; D009693:Nucleic Acid Hybridization; D056507:Paenibacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012820:Silage / Q000382:microbiology*; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry; D003313:Zea mays / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit