title: The effect of two fixation methods (TAF and DESS) on morphometric parameters of Aphelenchoides ritzemabosi.
abstract: Identification of nematode species by using conventional methods requires fixation of the isolated material and a suitable preparation for further analyses. Tentative identification using microscopic methods should also be performed prior to initiating molecular studies. In the literature, various methods are described for the preparation of nematodes from the genus Aphelenchoides for identification and microscopic studies. The most commonly used fixatives are formalin (Timm 1969; Szczygieł & Cid del Prado Vera 1981, Crozzoli et al. 2008, Khan et al. 2008), FAA (Wasilewska 1969; Vovlas et al. 2005, Khan et al. 2007) and TAF (Hooper 1958, Chizhov et al. 2006, Jagdale & Grewal 2006).
authors.affiliation: Research Institute of Horticulture, Konstytucji 3 Maja 1/3 Skierniewice, Poland. Museum and Institute of Zoology, Wilcza 64, 00-679 Warsaw, Poland.; Email: aneta.chalanska@inhort.pl.
authors.forename: Aneta
authors.initials: A
authors.lastname: Chałańska
authors.affiliation: Research Institute of Horticulture, Konstytucji 3 Maja 1/3 Skierniewice, Poland.; Email: aleksandra.bogumil@inhort.pl.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Bogumił
authors.affiliation: Museum and Institute of Zoology, Wilcza 64, 00-679 Warsaw, Poland.; Email: tmalewski@miiz.waw.pl.
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Malewski
authors.affiliation: Museum and Institute of Zoology, Wilcza 64, 00-679 Warsaw, Poland.; Email: k.kowalewska@miiz.waw.pl.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Kowalewska
mesh_terms: D000825:Animal Structures / Q000033:anatomy & histology / Q000737:chemistry / Q000254:growth & development; D000818:Animals; D049628:Body Size; D005260:Female; D005404:Fixatives / Q000737:chemistry*; D009929:Organ Size; D016707:Tissue Fixation / Q000295:instrumentation* / Q000379:methods; D017187:Tylenchida / Q000033:anatomy & histology* / Q000737:chemistry* / Q000254:growth & development
chemical_list: D005404:Fixatives