title: Hierarchical Fabrication of Engineered Vascularized Bone Biphasic Constructs via Dual 3D Bioprinting: Integrating Regional Bioactive Factors into Architectural Design.
abstract: A biphasic artificial vascularized bone construct with regional bioactive factors is presented using dual 3D bioprinting platform technique, thereby forming a large functional bone grafts with organized vascular networks. Biocompatible mussel-inspired chemistry and "thiol-ene" click reaction are used to regionally immobilize bioactive factors during construct fabrication for modulating or improving cellular events.
authors.affiliation: Department of Mechanical and Aerospace Engineering, The George Washington University, 3590 Science and Engineering Hall 800 22nd Street NW, Washington, DC, 20052, USA.
authors.forename: Haitao
authors.initials: H
authors.lastname: Cui
authors.affiliation: Department of Mechanical and Aerospace Engineering, The George Washington University, 3590 Science and Engineering Hall 800 22nd Street NW, Washington, DC, 20052, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Department of Mechanical and Aerospace Engineering, The George Washington University, 3590 Science and Engineering Hall 800 22nd Street NW, Washington, DC, 20052, USA.
authors.forename: Margaret
authors.initials: M
authors.lastname: Nowicki
authors.affiliation: Department of Mechanical and Aerospace Engineering, The George Washington University, 3590 Science and Engineering Hall 800 22nd Street NW, Washington, DC, 20052, USA.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Harvard-MIT Division of Health Sciences and Technology, Wyss Institute for Biologically Inspired Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 65 Landsdowne Street, Cambridge, MA, 02139, USA.
authors.forename: Ali
authors.initials: A
authors.lastname: Khademhosseini
authors.affiliation: Department of Mechanical and Aerospace Engineering, The George Washington University, 3590 Science and Engineering Hall 800 22nd Street NW, Washington, DC, 20052, USA. lgzhang@gwu.edu.|Department of Biomedical Engineering, The George Washington University, Washington DC, 20052, USA. lgzhang@gwu.edu.|Department of Medicine, The George Washington University, Washington DC, 20052, USA. lgzhang@gwu.edu.
authors.forename: Lijie Grace
authors.initials: LG
authors.lastname: Zhang
mesh_terms: D001842:Bone and Bones / Q000098:blood supply / Q000166:cytology / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D066330:Printing, Three-Dimensional; D023822:Tissue Engineering
keywords: click reaction; dual 3D bioprinting; mussel-inspired chemistry; regional immobilization; vascularized complex tissue