title: Genome sequence of a novel mitovirus identified in the phytopathogenic fungus Alternaria arborescens.
abstract: The phytopathogenic fungus Alternaria spp. contains a variety of double-stranded RNA (dsRNA) elements of different sizes. Detailed analysis of next-generation sequencing data obtained using dsRNA purified from Alternaria arborescens, from which we had previously found Alternaria arborescens victorivirus 1, revealed the presence of another mycoviral-like dsRNA of approximately 2.5 kbp in length. When using the fungal mitochondrial genetic code, this dsRNA has a single open reading frame that potentially encodes an RNA-dependent RNA polymerase (RdRp) with significant to sequence similarity to those of viruses of the genus Mitovirus. Moreover, both the 5'- and 3'-untranslated regions have the potential to fold into stable stem-loop structures, which is characteristic of mitoviruses. Pairwise comparisons and phylogenetic analysis of the deduced amino acid sequences of RdRp indicated that the virus we identified in A. arborescens is a distinct member of the genus Mitovirus in the family Narnaviridae, designated as "Alternaria arborescens mitovirus 1" (AaMV1).
authors.affiliation: Laboratory of Plant Pathology, Graduate School of Agriculture, Tokyo University of Agriculture and Technology (TUAT), 3-5-8 Saiwaicho, Fuchu, Tokyo, 183-8509, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Komatsu
authors.affiliation: Research and Education Center for Prevention of Global Infectious Diseases of Animals, Tokyo University of Agriculture and Technology (TUAT), 3-5-8 Saiwaicho, Fuchu, Tokyo, 183-8509, Japan.
authors.forename: Yukie
authors.initials: Y
authors.lastname: Katayama
authors.affiliation: Research and Education Center for Prevention of Global Infectious Diseases of Animals, Tokyo University of Agriculture and Technology (TUAT), 3-5-8 Saiwaicho, Fuchu, Tokyo, 183-8509, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Omatsu
authors.affiliation: Research and Education Center for Prevention of Global Infectious Diseases of Animals, Tokyo University of Agriculture and Technology (TUAT), 3-5-8 Saiwaicho, Fuchu, Tokyo, 183-8509, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Mizutani
authors.affiliation: Laboratory of Molecular and Cellular Biology, Graduate School of Agriculture, Tokyo University of Agriculture and Technology (TUAT), 3-5-8 Saiwaicho, Fuchu, Tokyo, 183-8509, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Fukuhara
authors.affiliation: Laboratory of Plant Pathology, Faculty of Agriculture, Tottori University, 4-101 Koyama-Minami, Tottori, 680-8553, Japan.
authors.forename: Motoichiro
authors.initials: M
authors.lastname: Kodama
authors.affiliation: Laboratory of Plant Pathology, Graduate School of Agriculture, Tokyo University of Agriculture and Technology (TUAT), 3-5-8 Saiwaicho, Fuchu, Tokyo, 183-8509, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Arie
authors.affiliation: Laboratory of Plant Pathology, Graduate School of Agriculture, Tokyo University of Agriculture and Technology (TUAT), 3-5-8 Saiwaicho, Fuchu, Tokyo, 183-8509, Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Teraoka
authors.affiliation: Laboratory of Molecular and Cellular Biology, Graduate School of Agriculture, Tokyo University of Agriculture and Technology (TUAT), 3-5-8 Saiwaicho, Fuchu, Tokyo, 183-8509, Japan. hmori714@cc.tuat.ac.jp.
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Moriyama
mesh_terms: D000528:Alternaria / Q000821:virology*; D000067148:Fungal Viruses / Q000235:genetics*; D016679:Genome, Viral; D010802:Phylogeny