title: Haplotypes of the TaGS5-A1 Gene Are Associated with Thousand-Kernel Weight in Chinese Bread Wheat.
abstract: In previous work, we cloned TaGS5 gene and found the association of TaGS5-A1 alleles with agronomic traits. In this study, the promoter sequence of the TaGS5-A1 gene was isolated from bread wheat. Sequencing results revealed that a G insertion was found in position -1925 bp of the TaGS5-A1 gene (Reference to ATG), which occurred in the Sp1 domain of the promoter sequence. Combined with previous single nucleotide polymorphism (SNP) in the TaGS5-A1 exon sequence, four genotypes were formed at the TaGS5-A1 locus and were designated as TaGS5-A1a-a, TaGS5-A1a-b, TaGS5-A1b-a, and TaGS5-A1b-b, respectively. Analysis of the association of TaGS5-A1 alleles with agronomic traits indicated that cultivars with the TaGS5-A1a-b allele possessed significantly higher thousand-kernel weight (TKW) and lower plant height than cultivars with the TaGS5-A1a-a allele, and cultivars with the TaGS5-A1b-b allele showed higher TKW than cultivars with the TaGS5-A1b-a allele. The differences of these traits between the TaGS5-A1a-a and TaGS5-A1a-b alleles were larger than those of the TaGS5-A1b-a and TaGS5-A1b-b alleles, suggesting that the -1925G insertion plays the more important role in TaGS5-A1a genotypes than in TaGS5-A1b genotypes. qRT-PCR indicated that TaGS5-A1b-b possessed the significantly highest expression level among four TaGS5-A1 haplotypes in mature seeds and further showed a significantly higher expression level than TaGS5-A1b-a at five different developmental stages of the seeds, suggesting that high expression of TaGS5-A1 was positively associated with high TKW in bread wheat. This study could provide a relatively superior genotype in view of TKW in wheat breeding programs and could also provide important information for dissection of the regulatory mechanism of the yield-related traits.
authors.affiliation: National Key Laboratory of Wheat and Corn Crop Science, Collaborative Innovation Center of Henan Grain Crops, Agronomy College, Henan Agricultural University Zhengzhou, China.
authors.forename: Shasha
authors.initials: S
authors.lastname: Wang
authors.affiliation: National Key Laboratory of Wheat and Corn Crop Science, Collaborative Innovation Center of Henan Grain Crops, Agronomy College, Henan Agricultural University Zhengzhou, China.
authors.forename: Xuefang
authors.initials: X
authors.lastname: Yan
authors.affiliation: National Key Laboratory of Wheat and Corn Crop Science, Collaborative Innovation Center of Henan Grain Crops, Agronomy College, Henan Agricultural University Zhengzhou, China.
authors.forename: Yongyan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: National Key Laboratory of Wheat and Corn Crop Science, Collaborative Innovation Center of Henan Grain Crops, Agronomy College, Henan Agricultural University Zhengzhou, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Liu
authors.affiliation: National Key Laboratory of Wheat and Corn Crop Science, Collaborative Innovation Center of Henan Grain Crops, Agronomy College, Henan Agricultural University Zhengzhou, China.
authors.forename: Dangqun
authors.initials: D
authors.lastname: Cui
authors.affiliation: National Key Laboratory of Wheat and Corn Crop Science, Collaborative Innovation Center of Henan Grain Crops, Agronomy College, Henan Agricultural University Zhengzhou, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
keywords: TaGS5-A1 gene; bread wheat; single nucleotide polymorphism; thousand-kernel weight; yield-related traits