title: Generating Cell Targeting Aptamers for Nanotheranostics Using Cell-SELEX.
abstract: Detecting and understanding changes in cell conditions on the molecular level is of great importance for the accurate diagnosis and timely therapy of diseases. Cell-based SELEX (Systematic Evolution of Ligands by EXponential enrichment), a foundational technology used to generate highly-specific, cell-targeting aptamers, has been increasingly employed in studies of molecular medicine, including biomarker discovery and early diagnosis/targeting therapy of cancer. In this review, we begin with a mechanical description of the cell-SELEX process, covering aptamer selection, identification and identification, and aptamer characterization; following this introduction is a comprehensive discussion of the potential for aptamers as targeting moieties in the construction of various nanotheranostics. Challenges and prospects for cell-SELEX and aptamer-based nanotheranostic are also discussed.
authors.affiliation: 1. Department of Cardiology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China.
authors.forename: Yifan
authors.initials: Y
authors.lastname: Lyu
authors.affiliation: 1. Department of Cardiology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China.
authors.forename: Guang
authors.initials: G
authors.lastname: Chen
authors.affiliation: 1. Department of Cardiology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China.
authors.forename: Dihua
authors.initials: D
authors.lastname: Shangguan
authors.affiliation: 1. Department of Cardiology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China.
authors.forename: Liqin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: 1. Department of Cardiology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Wan
authors.affiliation: 1. Department of Cardiology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: 1. Department of Cardiology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: 1. Department of Cardiology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China.
authors.forename: Lian
authors.initials: L
authors.lastname: Duan
authors.affiliation: 1. Department of Cardiology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.affiliation: 1. Department of Cardiology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China.
authors.forename: Mingxu
authors.initials: M
authors.lastname: You
authors.affiliation: 1. Department of Cardiology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.affiliation: 4. Department of Chemistry and Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, Health Cancer Center, UF Genetics Institute, McKnight Brain Institute, University of Florida, Gainesville, FL 32611-7200, USA.
authors.forename: Weihong
authors.initials: W
authors.lastname: Tan
mesh_terms: D000818:Animals; D003584:Cytological Techniques / Q000379:methods*; D006801:Humans; D052156:SELEX Aptamer Technique; D000068936:Theranostic Nanomedicine / Q000379:methods*
keywords: Aptamers; Cell-SELEX; DNA network.; aptamer drug conjugates; nanotheranostic