title: In vitro gastrointestinal resistance of Lactobacillus acidophilus La-5 and Bifidobacterium animalis Bb-12 in soy and/or milk-based synbiotic apple ice creams.
abstract: The viability and resistance to simulated gastrointestinal (GI) conditions of Lactobacillus acidophilus La-5 and Bifidobacterium animalis Bb-12 in synbiotic ice creams, in which milk was replaced by soy extract and/or whey protein isolate (WPI) with inulin, were investigated. The ice creams were showed to be satisfactory vehicles for La-5 and Bb-12 (populations around 7.5logCFU/g), even after the whole storage period (84days/-18°C). In all formulations, the propidium monoazide qPCR (PMA-qPCR) analysis demonstrated that probiotics could resist the in vitro GI assay, with significant survival levels, achieving survival rates exceeding 50%. Additionally, scanning electron microscopy images evidenced cells with morphological differences, suggesting physiological changes in response to the induced stress during the in vitro assay. Although all formulations provided resistance to the probiotic strains under GI stress, the variation found in probiotic survival suggests that GI tolerance is indeed affected by the choice of the food matrix.
authors.affiliation: Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 580, B16, 05508-000 São Paulo, SP, Brazil.
authors.forename: Natalia Silva
authors.initials: NS
authors.lastname: Matias
authors.affiliation: Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 580, B16, 05508-000 São Paulo, SP, Brazil.
authors.forename: Marina
authors.initials: M
authors.lastname: Padilha
authors.affiliation: Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 580, B16, 05508-000 São Paulo, SP, Brazil.
authors.forename: Raquel
authors.initials: R
authors.lastname: Bedani
authors.affiliation: Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 580, B16, 05508-000 São Paulo, SP, Brazil. Electronic address: susaad@usp.br.
authors.forename: Susana Marta Isay
authors.initials: SMI
authors.lastname: Saad
mesh_terms: D000818:Animals; D001386:Azides; D000069982:Bifidobacterium animalis / Q000378:metabolism*; D003080:Cold Temperature; D005503:Food Additives; D005744:Gastric Acid / Q000378:metabolism*; D000069196:Gastrointestinal Microbiome; D007054:Ice Cream / Q000382:microbiology*; D007444:Inulin / Q000378:metabolism; D007779:Lactobacillus acidophilus / Q000378:metabolism*; D027845:Malus / Q000382:microbiology*; D008892:Milk / Q000382:microbiology; D019936:Probiotics / Q000032:analysis; D011419:Propidium / Q000031:analogs & derivatives; D013025:Glycine max / Q000382:microbiology*; D058616:Synbiotics; D000067816:Whey Proteins
chemical_list: D001386:Azides; D005503:Food Additives; D000067816:Whey Proteins; C533957:propidium monoazide; D011419:Propidium; D007444:Inulin
keywords: Functional food; In vitro gastrointestinal survival; Prebiotic; Probiotic; Propidium monoazide qPCR; SEM