title: Quality improvement and geographical indication of cachaça (Brazilian spirit) by using locally selected yeast strains.
abstract: OBJECTIVE
  In order to improve the quality and to create a biological basis for obtainment of the protected denomination of origin (PDO), indigenous yeast were isolated and characterized for use in Salinas city (the Brazilian region of quality cachaça production).
  
  
  METHODS
  Seven thousand and two hundred yeast colonies from 15 Salinas city distilleries were screened based on their fermentative behaviour and the physicochemical composition of cachaça. Molecular polymorphic analyses were performed to characterize these isolates.
  
  
  RESULTS
  Two Saccharomyces cerevisiae strains (nos. 678 and 680) showed appropriate characteristics to use in the cachaça production: low levels of acetaldehyde and methanol, and high ethyl lactate/ethyl acetate ratio respectively. They also presented polymorphic characteristics more closely related between themselves even when compared to other strains from Salinas.
  
  
  CONCLUSIONS
  The application of selected yeast to cachaça production can contribute for the improvement of the quality product as well as be used as a natural marker for PDO.
  
  
  CONCLUSIONS
  This study suggests that the use of selected yeast strains could contribute to obtain a cachaça similar to those produced traditionally, while getting wide acceptation in the market, yet presenting more homogeneous organoleptic characteristics, and thus contributing to the PDO implementation.
authors.affiliation: Laboratório de Biologia Celular e Molecular, Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil.|Laboratório de Análises Físico - Químicas, Departamento de Química, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil.
authors.forename: E A
authors.initials: EA
authors.lastname: Barbosa
authors.affiliation: Laboratório de Biologia Celular e Molecular, Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil.|Laboratório de Análises Físico - Químicas, Departamento de Química, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil.
authors.forename: M T
authors.initials: MT
authors.lastname: Souza
authors.affiliation: Laboratório de Biologia Celular e Molecular, Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil.
authors.forename: R H S
authors.initials: RH
authors.lastname: Diniz
authors.affiliation: Laboratório de Biologia Celular e Molecular, Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil.
authors.forename: F
authors.initials: F
authors.lastname: Godoy-Santos
authors.affiliation: Laboratório de Biologia Celular e Molecular, Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil.
authors.forename: F
authors.initials: F
authors.lastname: Faria-Oliveira
authors.affiliation: Laboratório de Biologia Celular e Molecular, Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil.
authors.forename: L F M
authors.initials: LF
authors.lastname: Correa
authors.affiliation: Cerlev - Projetos e Inovação na Biotecnologia da Fermentação Ltda., Ouro Preto, MG, Brazil.
authors.forename: F
authors.initials: F
authors.lastname: Alvarez
authors.affiliation: Campus Salinas, Instituto Federal Norte de Minas Gerais, Salinas, MG, Brazil.
authors.forename: M X
authors.initials: MX
authors.lastname: Coutrim
authors.affiliation: Campus Salinas, Instituto Federal Norte de Minas Gerais, Salinas, MG, Brazil.
authors.forename: R J C F
authors.identifier: http://orcid.org/0000-0003-1940-3304
authors.initials: RJ
authors.lastname: Afonso
authors.affiliation: Laboratório de Biologia Celular e Molecular, Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil.
authors.forename: I M
authors.initials: IM
authors.lastname: Castro
authors.affiliation: Laboratório de Biologia Celular e Molecular, Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil. rlbrand@nupeb.ufop.br.
authors.forename: R L
authors.initials: RL
authors.lastname: Brandão
mesh_terms: D000079:Acetaldehyde / Q000032:analysis / Q000378:metabolism; D000434:Alcoholic Beverages / Q000032:analysis / Q000382:microbiology*; D001938:Brazil; D005285:Fermentation; D000432:Methanol / Q000032:analysis / Q000378:metabolism; D058996:Quality Improvement; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000079:Acetaldehyde; D000432:Methanol
keywords: beverages; fermentation biotechnology; genotyping; quality control; yeasts