title: Suppression of Jasmonic Acid-Mediated Defense by Viral-Inducible MicroRNA319 Facilitates Virus Infection in Rice.
abstract: MicroRNAs (miRNAs) are pivotal modulators of plant development and host-virus interactions. However, the roles and action modes of specific miRNAs involved in viral infection and host susceptibility remain largely unclear. In this study, we show that Rice ragged stunt virus (RRSV) infection caused increased accumulation of miR319 but decreased expression of miR319-regulated TCP (TEOSINTE BRANCHED/CYCLOIDEA/PCF) genes, especially TCP21, in rice plants. Transgenic rice plants overexpressing miR319 or downregulating TCP21 exhibited disease-like phenotypes and showed significantly higher susceptibility to RRSV in comparison with the wild-type plants. In contrast, only mild disease symptoms were observed in RRSV-infected lines overexpressing TCP21 and especially in the transgenic plants overexpressing miR319-resistant TCP21. Both RRSV infection and overexpression of miR319 caused the decreased endogenous jasmonic acid (JA) levels along with downregulated expression of JA biosynthesis and signaling-related genes in rice. However, treatment of rice plants with methyl jasmonate alleviated disease symptoms caused by RRSV and reduced virus accumulation. Taken together, our results suggest that the induction of miR319 by RRSV infection in rice suppresses JA-mediated defense to facilitate virus infection and symptom development.
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
authors.forename: Zuomei
authors.initials: Z
authors.lastname: Ding
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
authors.forename: Kangcheng
authors.initials: K
authors.lastname: Wu
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Yang
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Shi
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Liu
authors.affiliation: The State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Zhao
authors.affiliation: The State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Zhirui
authors.initials: Z
authors.lastname: Yang
authors.affiliation: The State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
authors.forename: Luping
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wei
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
authors.forename: Zhenguo
authors.initials: Z
authors.lastname: Du
authors.affiliation: Plant Protection Institute, Hebei Academy of Agriculture and Forestry Sciences, Baoding 071000, China.
authors.forename: Aihong
authors.initials: A
authors.lastname: Zhang
authors.affiliation: Plant Protection Institute, Hebei Academy of Agriculture and Forestry Sciences, Baoding 071000, China.
authors.forename: Hongqin
authors.initials: H
authors.lastname: Miao
authors.affiliation: The State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China. Electronic address: wuzujian@126.com.
authors.forename: Zujian
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; The State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China. Electronic address: wujianguo81@126.com.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Wu
mesh_terms: D003517:Cyclopentanes / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D012275:Oryza / Q000235:genetics / Q000378:metabolism* / Q000821:virology*; D054883:Oxylipins / Q000378:metabolism*; D010935:Plant Diseases / Q000235:genetics / Q000821:virology; D010942:Plant Viruses / Q000472:pathogenicity; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism / Q000821:virology; D012087:Reoviridae / Q000472:pathogenicity
chemical_list: D003517:Cyclopentanes; D035683:MicroRNAs; D054883:Oxylipins; C011006:jasmonic acid
keywords: RRSV; TCP21; jasmonic acid; miR319; rice ragged stunt virus; viral infection