title: Molecular characterization and expression analysis of AMPK α subunit isoform genes from Scophthalmus maximus responding to salinity stress.
abstract: AMP-activated protein kinase (AMPK) is a highly conserved and multi-functional protein kinase that plays important roles in both intracellular energy balance and cellular stress response. In the present study, molecular characterization, tissue distribution and gene expression levels of the AMPK α1 and α2 genes from turbot (Scophthalmus maximus) under salinity stress are described. The complete coding regions of the AMPK α1 and α2 genes were isolated from turbot through degenerate primers in combination with RACE using muscle cDNA. The complete coding regions of AMPK α1 (1722 bp) and α2 (1674 bp) encoded 573 and 557 amino acids peptides, respectively. Multiple alignments, structural analysis and phylogenetic tree construction indicated that S. maximus AMPK α1 and α2 shared a high amino acid identity with other species, especially fish. AMPK α1 and α2 genes could be detected in all tested tissues, indicating that they are constitutively expressed. Salinity challenges significantly altered the gene expression levels of AMPK α1 and α2 mRNA in a salinity- and time-dependent manners in S. maximus gill tissues, suggesting that AMPK α1 and α2 played important roles in mediating the salinity stress in S. maximus. The expression levels of AMPK α1 and α2 mRNA were a positive correlation with gill Na+, K+-ATPase activities. These findings will aid our understanding of the molecular mechanism of juvenile turbot in response to environmental salinity changes.
authors.affiliation: National Engineering Research Center for Marine Aquaculture, Zhejiang Ocean University, Zhoushan, 316000, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zeng
authors.affiliation: National Technology System for Flatfish Culture Industry, Chinese Ministry of Agriculture, Qingdao, 266071, China.|Qingdao Key Laboratory for Marine Fish Breeding and Biology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.affiliation: National Engineering Research Center for Marine Aquaculture, Zhejiang Ocean University, Zhoushan, 316000, China.
authors.forename: Chang-Wen
authors.initials: CW
authors.lastname: Wu
authors.affiliation: National Technology System for Flatfish Culture Industry, Chinese Ministry of Agriculture, Qingdao, 266071, China. leijilin@seacul.com.|Qingdao Key Laboratory for Marine Fish Breeding and Biology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China. leijilin@seacul.com.
authors.forename: Ji-Lin
authors.initials: JL
authors.lastname: Lei
authors.affiliation: National Engineering Research Center for Marine Aquaculture, Zhejiang Ocean University, Zhoushan, 316000, China.
authors.forename: Mei-Ying
authors.initials: MY
authors.lastname: Xu
authors.affiliation: National Engineering Research Center for Marine Aquaculture, Zhejiang Ocean University, Zhoushan, 316000, China.
authors.forename: Ai-Yi
authors.initials: AY
authors.lastname: Zhu
authors.affiliation: National Engineering Research Center for Marine Aquaculture, Zhejiang Ocean University, Zhoushan, 316000, China.
authors.forename: Jian-She
authors.initials: JS
authors.lastname: Zhang
authors.affiliation: College of Ocean and Earth Sciences, Xiamen University, Xiamen, 361005, China.
authors.forename: Wan-Shu
authors.initials: WS
authors.lastname: Hong
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D005412:Flatfishes / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D005880:Gills / Q000201:enzymology; D010802:Phylogeny; D020033:Protein Isoforms / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D054712:Salinity; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013312:Stress, Physiological / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; D055372:AMP-Activated Protein Kinases; D000254:Sodium-Potassium-Exchanging ATPase
keywords: AMPK; Na+ K+-ATPase activity; RACE; Salinity; Scophthalmus maximus