title: Hepatoprotective properties of oleanolic and ursolic acids in antitubercular drug-induced liver damage.
abstract: OBJECTIVE
  To estimate to what extent the mixture of ursolic acid and oleanolic acid, in addition to the antitubercular standard regime, affects the hepatotoxicity profile.
  
  
  METHODS
  Liver injury was induced in male BALB/c mice by administering, per os and daily for 11 weeks, a combination of anti-Tubercular (anti-TB) agents Rifampicin (10 mg/kg), Isoniazid (10 mg/kg), and Pyrazinamide (30 mg/kg). The ursolic acid and oleanolic acid mixture at doses of 100 or 200 μg/mouse/day was subcutaneously injected throughout the entire study period (11 weeks). Biochemical and hematological analysis was supplemented by liver histological examination.
  
  
  RESULTS
  Animals treated with the mixture of triterpenic acids exhibited significantly decreased aspartate transaminase and alanine aminotransferase levels and amelioration of the histopathological alterations produced by the anti-TB drugs.
  
  
  CONCLUSIONS
  The triterpene mixture was able to prevent the steatosis induced by the anti-TB drugs.
authors.affiliation: Unidad de Investigación Médica en Farmacología (UIMF), UMAE Hospital de Especialidades, Centro Médico Nacional-Siglo XXI (CMN-SXXI), Instituto Mexicano del Seguro Social (IMSS), México City, Mexico.
authors.forename: Gabriel A
authors.initials: GA
authors.lastname: Gutiérrez-Rebolledo
authors.affiliation: Servicio de Patología, UMAE Hospital de Pediatría, CMN-SXXI, IMSS, México City, Mexico.
authors.forename: A Georgina
authors.initials: AG
authors.lastname: Siordia-Reyes
authors.affiliation: Centro de Diagnóstico en Metabolismo Energético y Medicina Mitocondrial, A.C., México City, Mexico.
authors.forename: Mariana
authors.initials: M
authors.lastname: Meckes-Fischer
authors.affiliation: Unidad de Investigación Médica en Farmacología (UIMF), UMAE Hospital de Especialidades, Centro Médico Nacional-Siglo XXI (CMN-SXXI), Instituto Mexicano del Seguro Social (IMSS), México City, Mexico. Electronic address: adelinajim08@prodigy.net.mx.
authors.forename: Adelina
authors.initials: A
authors.lastname: Jiménez-Arellanes
keywords: Antitubercular drugs; Hepatoprotector effect; Oleanolic acid; Triterpenes; Ursolic acid