title: Mutation of Rice Early Flowering3.1 (OsELF3.1) delays leaf senescence in rice.
abstract: In Arabidopsis, EARLY FLOWERING3 (ELF3) has pivotal roles in controlling circadian rhythm and photoperiodic flowering. In addition, ELF3 negatively regulates leaf senescence by repressing the transcription of PHYTOCHROME-INTERACTING FACTOR4 (PIF4) and PHYTOCHROME-INTERACTING FACTOR5 (PIF5); elf3 mutants senesce earlier and ELF3-overexpressing (ELF3-OX) plants senesce later than wild type (WT). Here, we show that in contrast to Arabidopsis ELF3, which represses senescence, the rice homolog OsELF3.1 promotes leaf senescence; oself3.1 mutants showed delayed senescence and OsELF3.1-OX plants senesced earlier under both dark-induced and natural senescence conditions. Microarray analysis revealed that in the senescing leaves, a number of senescence-associated genes, phytohormone-related genes, and NAC and WRKY family genes (OsNAP, ONAC106, and OsWRKY42) were differentially expressed in oself3.1 mutants compared with WT. Interestingly, we found that Arabidopsis plants overexpressing OsELF3.1 show delayed leaf senescence, produce short petioles, and flower late in long days, just like Arabidopsis ELF3-OX plants. This demonstrates that the regulatory functions of ELF3 and OsELF3.1 are conserved between Arabidopsis and rice, but the downstream regulatory cascades have opposite effects.
authors.affiliation: Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Republic of Korea.|Graduate school of Agricultural and Life Sciences, Biotechnology Research Center, The University of Tokyo, Tokyo, 113-8657, Japan.
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Sakuraba
authors.affiliation: Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Republic of Korea.
authors.forename: Su-Hyun
authors.initials: SH
authors.lastname: Han
authors.affiliation: Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Republic of Korea.
authors.forename: Hyun-Jung
authors.initials: HJ
authors.lastname: Yang
authors.affiliation: Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Republic of Korea.
authors.forename: Weilan
authors.initials: W
authors.lastname: Piao
authors.affiliation: Department of Plant Science, Plant Genomics and Breeding Institute, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Republic of Korea. ncpaek@snu.ac.kr.|Crop Biotechnology Institute, GreenBio Science and Technology, Seoul National University, Pyeongchang, 232-916, Republic of Korea. ncpaek@snu.ac.kr.
authors.forename: Nam-Chon
authors.identifier: http://orcid.org/0000-0002-9827-2287
authors.initials: NC
authors.lastname: Paek
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D035264:Flowers / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D018506:Gene Expression Regulation, Plant; D009154:Mutation; D012275:Oryza / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism / Q000502:physiology
keywords: Arabidopsis ELF3; Leaf senescence; OsELF3.1; Rice; Transcriptional regulation