title: Renal epithelial miR-205 expression correlates with disease severity in a mouse model of congenital obstructive nephropathy.
abstract: Congenital obstructive nephropathy (CON) is a leading cause of pediatric chronic kidney disease (CKD). Despite optimal surgical and medical care, there is a high rate of CKD progression. Better understanding of molecular and cellular changes is needed to facilitate development of improved biomarkers and novel therapeutic approaches in CON.
  
  
  
  The megabladder (mgb) mouse is an animal model of CKD with impaired bladder emptying, hydronephrosis, and progressive renal injury. In this study, we characterize a particular microRNA, miR-205, whose expression changes with the degree of hydronephrosis in the mgb(-/-) kidney.
  
  
  
  Expression of miR-205 is progressively increased in the adult mgb(-/-) mouse with worsening severity of hydronephrosis. miR-205 expression is correlated with altered expression of cytokeratins and uroplakins, which are markers of cellular differentiation in urothelium. We describe the spatial pattern of miR-205 expression, including increased expression in renal urothelium and novel miR-205 expression in medullary collecting duct epithelium in the congenitally obstructed kidney.
  
  
  
  miR-205 is increased with severity of CON and CKD in the mgb(-/-) mouse and may regulate urothelial differentiation.
authors.affiliation: Center for Clinical and Translational Research, The Research Institute at Nationwide Children's Hospital, Columbus, Ohio.
authors.forename: Michael E
authors.initials: ME
authors.lastname: Wilhide
authors.affiliation: Center for Clinical and Translational Research, The Research Institute at Nationwide Children's Hospital, Columbus, Ohio.
authors.forename: James D
authors.initials: JD
authors.lastname: Feller
authors.affiliation: Center for Clinical and Translational Research, The Research Institute at Nationwide Children's Hospital, Columbus, Ohio.
authors.forename: Birong
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Urology, University of Louisville, Louisville, Kentucky.
authors.forename: Ahmad Z
authors.initials: AZ
authors.lastname: Mohamed
authors.affiliation: Center for Clinical and Translational Research, The Research Institute at Nationwide Children's Hospital, Columbus, Ohio.|Department of Pediatrics, The Ohio State University, Columbus, Ohio.
authors.forename: Brian
authors.initials: B
authors.lastname: Becknell
authors.affiliation: Center for Molecular and Human Genetics, The Research Institute at Nationwide Children's Hospital, Columbus, Ohio.
authors.forename: Ashley R
authors.initials: AR
authors.lastname: Jackson
authors.affiliation: Center for Molecular and Human Genetics, The Research Institute at Nationwide Children's Hospital, Columbus, Ohio.
authors.forename: Kirk M
authors.initials: KM
authors.lastname: McHugh
authors.affiliation: Center for Clinical and Translational Research, The Research Institute at Nationwide Children's Hospital, Columbus, Ohio.|Department of Pediatrics, The Ohio State University, Columbus, Ohio.
authors.forename: Susan E
authors.initials: SE
authors.lastname: Ingraham
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D002454:Cell Differentiation; D004195:Disease Models, Animal; D018450:Disease Progression; D004848:Epithelium / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D006869:Hydronephrosis / Q000097:blood; D007633:Keratins / Q000097:blood; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D007674:Kidney Diseases / Q000151:congenital* / Q000235:genetics / Q000503:physiopathology; D007676:Kidney Failure, Chronic / Q000097:blood; D007685:Kidney Tubules, Collecting / Q000378:metabolism; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D035683:MicroRNAs / Q000235:genetics*; D019108:Tight Junctions; D060165:Uroplakins / Q000097:blood; D019459:Urothelium / Q000378:metabolism / Q000473:pathology
chemical_list: D015415:Biomarkers; C576666:MIRN205 microRNA, mouse; D035683:MicroRNAs; D060165:Uroplakins; D007633:Keratins