title: Detection of Proteins Binding to the Promoter Region DNA Using a Nonradioactive Gel-Retardation Assay.
abstract: Binding of nuclear proteins to the promoter region was studied by a nonradioactive gel-retardation assay. The procedure uses biotinylated oligonucleotides in combination with streptavidin and biotin-conjugated alkaline phosphatase. This method offers sensitivity comparable to radioactive detection, and the advantage of the high stability of probes. Moreover the hazards of usage associated with radiation are avoided.
authors.affiliation: a Department of Life and Health Science, Joetsu University of Education.
authors.forename: S
authors.initials: S
authors.lastname: Mitsunaga
authors.affiliation: b Laboratory of Rice Genetics Engineering, Hokuriku National Agricultural Experiment Station.
authors.forename: K
authors.initials: K
authors.lastname: Fukui
authors.affiliation: a Department of Life and Health Science, Joetsu University of Education.
authors.forename: H
authors.initials: H
authors.lastname: Ohyama
authors.affiliation: c BioScience Center, Nagoya University.
authors.forename: J
authors.initials: J
authors.lastname: Yamaguchi
keywords: DNA-binding protein; gel-retardation assay; nonradioactive