title: Integrated Electrowetting Nanoinjector for Single Cell Transfection.
abstract: Single cell transfection techniques are essential to understand the heterogeneity between cells. We have developed an integrated electrowetting nanoinjector (INENI) to transfect single cells. The high transfection efficiency, controlled dosage delivery and ease of INENI fabrication promote the widespread application of the INENI in cell transfection assays.
authors.affiliation: Department of Biomedical Engineering, University of California Irvine, California, USA.
authors.forename: Elaheh
authors.initials: E
authors.lastname: Shekaramiz
authors.affiliation: Department of Electrical Engineering, University of California Irvine, California, USA.
authors.forename: Ganeshkumar
authors.initials: G
authors.lastname: Varadarajalu
authors.affiliation: Manchester Institute of Biotechnology, the University of Manchester, Manchester, UK.
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Day
authors.affiliation: Department of Biomedical Engineering, University of California Irvine, California, USA.|Department of Electrical Engineering, University of California Irvine, California, USA.
authors.forename: H Kumar
authors.initials: HK
authors.lastname: Wickramasinghe
mesh_terms: D058874:Electrowetting / Q000379:methods*; D006801:Humans; D008845:Microinjections / Q000379:methods*; D059010:Single-Cell Analysis; D014162:Transfection