title: Impact of Lens Care Solutions on Protein Deposition on Soft Contact Lenses.
abstract: To evaluate the effect of four contemporary lens care solutions on total protein, total lysozyme, and active lysozyme extracted from three contact lens materials.
  
  
  
  Adapted contact lens wearers were recruited at three sites, and all subjects were randomly assigned to daily wear of either etafilcon A, galyfilcon A, or senofilcon A for 2 weeks. Four lens care solutions (Biotrue, OPTI-FREE PureMoist, RevitaLens OcuTec, and ClearCare) were used by each subject in random order with a new pair of lenses after a washout period between solutions of at least 4 days. After 2 weeks of daily wear, contact lenses were collected for analysis. Proteins were extracted from a subset of contact lenses (n = 568) and total protein, total lysozyme, and lysozyme activity were quantified using a modified Bradford assay, an enzyme-linked immunosorbent assay, and a micrococcal assay, respectively.
  
  
  
  Higher levels of total protein were extracted from etafilcon A when used with Biotrue compared to other solutions (p = 0.0001). There were higher levels of total lysozyme extracted from galyfilcon A lenses when used with PureMoist than with Biotrue or ClearCare (p < 0.006). Higher total lysozyme was extracted from senofilcon A when used with RevitaLens OcuTec compared to Biotrue (p = 0.002). Lower lysozyme activity was recovered from senofilcon A lenses with RevitaLens OcuTec when compared to all other care solutions (all p < 0.004). When Biotrue, PureMoist, or RevitaLens OcuTec were used, higher total lysozyme was extracted from galyfilcon A compared to senofilcon A (p < 0.01). When RevitaLens OcuTec was used, higher levels of active lysozyme were extracted from galyfilcon A compared to senofilcon A (p = 0.02).
  
  
  
  The ability of lens care solutions to remove protein from lenses varies depending upon the care solution composition and also the polymeric make-up of the contact lens material.
authors.affiliation: *PhD, BOptom †MSc ‡PhD, BSOptom, FAAO §PhD ∥PhD, BScOptom, PGCertOcTher, FAAO **MCOptom, BSc(Hons) ††PhD, MCOptom, FAAO ‡‡OD, PhD, FAAO §§OD, PhD, MPH, FAAO ∥∥PhD, FCOptom, FAAO Centre for Contact Lens Research, School of Optometry and Vision Science, University of Waterloo, Ontario, Canada (NBO, MH, LNS, DP, LWJ); Johnson and Johnson Vision Care, Inc., Jacksonville, Florida (CL); Eurolens Research, University of Manchester, Manchester, United Kingdom (SLS, PBM); The Ocular Surface Institute, College of Optometry, University of Houston, Houston, Texas (DAB); and School of Optometry, University of Alabama, Birmingham, Birmingham, Alabama (JJN).
authors.forename: Negar
authors.initials: N
authors.lastname: Babaei Omali
authors.forename: Miriam
authors.initials: M
authors.lastname: Heynen
authors.forename: Lakshman N
authors.initials: LN
authors.lastname: Subbaraman
authors.forename: Dominik
authors.initials: D
authors.lastname: Papinski
authors.forename: Carol
authors.initials: C
authors.lastname: Lakkis
authors.forename: Sarah L
authors.initials: SL
authors.lastname: Smith
authors.forename: Philip B
authors.initials: PB
authors.lastname: Morgan
authors.forename: David A
authors.initials: DA
authors.lastname: Berntsen
authors.forename: Jason J
authors.initials: JJ
authors.lastname: Nichols
authors.forename: Lyndon W
authors.initials: LW
authors.lastname: Jones
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D018639:Contact Lens Solutions / Q000494:pharmacology*; D003263:Contact Lenses, Hydrophilic; D004797:Enzyme-Linked Immunosorbent Assay; D004866:Equipment Contamination; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D011506:Proteins / Q000032:analysis*; D055815:Young Adult
chemical_list: D018639:Contact Lens Solutions; D011506:Proteins