title: [Expression and clinical significance of IL-33 in patients with non-small cell lung cancer].
abstract: Objective To investigate the expression of interleukin 33 (IL-33) in cancer and adjacent non-cancerous tissues from patients with non-small cell lung cancer (NSCLC). Methods Real-time quantitative PCR was performed to detect the mRNA expression of IL-33 in 61 pairs of cancerous and adjacent non-cancerous tissues. In addition, immunohistochemistry was used to evaluate the expression and location of IL-33 on paraffin sections in selected 12 cases with different pathological types, to analyze the correlation of IL-33 expression with clinicopathological variables and overall survival. Results The expression of IL-33 mRNA in cancer tissues was significantly lower than that in adjacent non-cancerous tissues. The immunohistochemical results showed that IL-33 protein was mainly localized in the nucleus, and was obviously down-regulated in NSCLC. Besides, the expression of IL-33 was associated with histological type, but was not associated with age, gender, smoking history, differentiation status and pathological TNM stage. According to the Kaplan-Meier analysis, the expression of IL-33 mRNA was evidently associated with post-operative overall survival of patients suffering from NSCLC. Conclusion IL-33 may play an important role in the development of NSCLC and the targeted therapy.
authors.affiliation: School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, China.
authors.forename: Yuehua
authors.initials: Y
authors.lastname: Feng
authors.affiliation: School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, China. *Corresponding author, E-mail: xlyb99@163.com.
authors.forename: Yibei
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Comprehensive Laboratory, Third Affiliated Hospital, Soochow University, Changzhou 213003, China.
authors.forename: Guanghua
authors.initials: G
authors.lastname: Luo
authors.affiliation: Department of Cardiothoracic Surgery, Third Affiliated Hospital, Soochow University, Changzhou 213003, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Cardiothoracic Surgery, Third Affiliated Hospital, Soochow University, Changzhou 213003, China.
authors.forename: Pengyi
authors.initials: P
authors.lastname: Yu
authors.affiliation: Department of Cardiothoracic Surgery, Third Affiliated Hospital, Soochow University, Changzhou 213003, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zheng
mesh_terms: D000328:Adult; D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D000067596:Interleukin-33 / Q000235:genetics* / Q000378:metabolism; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D000067596:Interleukin-33