title: Investigation of contactless detection using a giant magnetoresistance sensor for detecting prostate specific antigen.
abstract: This paper presents a contactless detection method for detecting prostate specific antigen with a giant magnetoresistance sensor. In contactless detection case, the prostate specific antigen sample preparation was separated from the sensor that prevented the sensor from being immersed in chemical solvents, and made the sensor implementing in immediately reuse without wash. Experimental results showed that applied an external magnetic field in a range of 50 Oe to 90 Oe, Dynabeads with a concentration as low as 0.1 μg/mL can be detected by this system and could give an approximate quantitation to the logarithmic of Dynabeads concentration. Sandwich immunoassay was employed for preparing PSA samples. The PSA capture was implemented on a gold film modified with a self-assembled monolayer and using biotinylated secondary antibody against PSA and streptavidinylated Dynabeads. With DC magnetic field in the range of 50 to 90 Oe, PSA can be detected with a detection limit as low as 0.1 ng/mL. Samples spiked with different concentrations of PSA can be distinguished clearly. Due to the contactless detection method, the detection system exhibited advantages such as convenient manipulation, reusable, inexpensive, small weight. So, this detection method was a promising candidate in biomarker detection, especially in point of care detection.
authors.affiliation: Key Laboratory for Thin Film and Microfabrication of the Ministry of Education, Department of Micro/Nano Electronics, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Dongchuan Road 800, Shanghai, 200240, China.
authors.forename: Xuecheng
authors.initials: X
authors.lastname: Sun
authors.affiliation: Key Laboratory for Thin Film and Microfabrication of the Ministry of Education, Department of Micro/Nano Electronics, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Dongchuan Road 800, Shanghai, 200240, China.
authors.forename: Shaotao
authors.initials: S
authors.lastname: Zhi
authors.affiliation: Key Laboratory for Thin Film and Microfabrication of the Ministry of Education, Department of Micro/Nano Electronics, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Dongchuan Road 800, Shanghai, 200240, China. leiqhd@sjtu.edu.cn.
authors.forename: Chong
authors.initials: C
authors.lastname: Lei
authors.affiliation: Key Laboratory for Thin Film and Microfabrication of the Ministry of Education, Department of Micro/Nano Electronics, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Dongchuan Road 800, Shanghai, 200240, China. yzhou@sjtu.edu.cn.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000906:Antibodies / Q000737:chemistry; D015415:Biomarkers / Q000032:analysis; D015374:Biosensing Techniques; D006801:Humans; D007118:Immunoassay; D057230:Limit of Detection; D008280:Magnetics / Q000295:instrumentation*; D008297:Male; D055616:Microtechnology; D017430:Prostate-Specific Antigen / Q000032:analysis*
chemical_list: D000906:Antibodies; D015415:Biomarkers; D017430:Prostate-Specific Antigen
keywords: Contactless; Dynabead; Giant magnetoresistance sensor; Prostate specific antigen