title: Multiplex Detection of IgG and IgM to Rift Valley Fever Virus Nucleoprotein, Nonstructural Proteins, and Glycoprotein in Ovine and Bovine.
abstract: A multiplex fluorescence microsphere immunoassay (FMIA) was used to detect bovine and ovine IgM and IgG antibodies to several Rift Valley fever virus (RVFV) proteins, including the major surface glycoprotein, Gn; the nonstructural proteins, NSs and NSm; and the nucleoprotein, N. Target antigens were assembled into a multiplex and tested in serum samples from infected wild-type RVFV or MP12, a modified live virus vaccine. As expected, the N protein was immunodominant and the best target for early detection of infection. Antibody activity against the other targets was also detected. The experimental results demonstrate the capabilities of FMIA for the detection of antibodies to RVFV structural and nonstructural proteins, which can be applied to future development and validation of diagnostic tests that can be used to differentiate vaccinated from infected animals.
authors.affiliation: 1 Department of Diagnostic Medicine/Pathobiology, Center of Excellence for Emerging and Zoonotic Animal Diseases (CEEZAD), College of Veterinary Medicine, Kansas State University , Manhattan, Kansas.|2 USDA-ARS Arthropod-Borne Animal Disease Research Unit (ABADRU), Center for Grain and Animal Health Research , Manhattan, Kansas.
authors.forename: Mohammad M
authors.initials: MM
authors.lastname: Hossain
authors.affiliation: 2 USDA-ARS Arthropod-Borne Animal Disease Research Unit (ABADRU), Center for Grain and Animal Health Research , Manhattan, Kansas.
authors.forename: William C
authors.initials: WC
authors.lastname: Wilson
authors.affiliation: 1 Department of Diagnostic Medicine/Pathobiology, Center of Excellence for Emerging and Zoonotic Animal Diseases (CEEZAD), College of Veterinary Medicine, Kansas State University , Manhattan, Kansas.
authors.forename: Bonto
authors.initials: B
authors.lastname: Faburay
authors.affiliation: 1 Department of Diagnostic Medicine/Pathobiology, Center of Excellence for Emerging and Zoonotic Animal Diseases (CEEZAD), College of Veterinary Medicine, Kansas State University , Manhattan, Kansas.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Richt
authors.affiliation: 2 USDA-ARS Arthropod-Borne Animal Disease Research Unit (ABADRU), Center for Grain and Animal Health Research , Manhattan, Kansas.
authors.forename: David S
authors.initials: DS
authors.lastname: McVey
authors.affiliation: 1 Department of Diagnostic Medicine/Pathobiology, Center of Excellence for Emerging and Zoonotic Animal Diseases (CEEZAD), College of Veterinary Medicine, Kansas State University , Manhattan, Kansas.
authors.forename: Raymond R
authors.initials: RR
authors.lastname: Rowland
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000821:virology*; D006023:Glycoproteins / Q000276:immunology; D007118:Immunoassay / Q000379:methods / Q000662:veterinary*; D007074:Immunoglobulin G / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood*; D009698:Nucleoproteins / Q000276:immunology; D011994:Recombinant Proteins; D012295:Rift Valley Fever / Q000097:blood / Q000175:diagnosis / Q000821:virology; D012296:Rift Valley fever virus / Q000276:immunology*; D012756:Sheep; D012757:Sheep Diseases / Q000097:blood / Q000821:virology*; D017361:Viral Nonstructural Proteins / Q000276:immunology; D015678:Viral Structural Proteins / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D006023:Glycoproteins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D009698:Nucleoproteins; D011994:Recombinant Proteins; D017361:Viral Nonstructural Proteins; D015678:Viral Structural Proteins
keywords: Fluorescence microsphere immunoassay; IgM antibody response; Recombinant RVFV proteins; Rift Valley fever virus