title: Self-assembly of Ni-NTA-modified β-annulus peptides into artificial viral capsids and encapsulation of His-tagged proteins.
abstract: β-Annulus peptides bearing Cys at the N-terminal from tomato bushy stunt virus were synthesised using a standard Fmoc-protected solid-phase method, and the peptide was modified with Ni-NTA at the N-terminal. The Ni-NTA-modified β-annulus peptide self-assembled into virus-like nanocapsules of approximately 40 nm in diameter. The critical aggregation concentration of these nanocapsules in 10 mM Tris-HCl buffer (pH 7.3) at 25 °C was 0.053 μM, which is 470 times lower than that of unmodified β-annulus peptides. Moreover, size exclusion chromatography of the peptide assembly indicated encapsulation of His-tagged green fluorescent protein in the Ni-NTA-modified artificial viral capsid.
authors.affiliation: Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, Tottori 680-8552, Japan. ma2ra-k@chem.tottori-u.ac.jp.
authors.forename: Kazunori
authors.initials: K
authors.lastname: Matsuura
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka 819-0395, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Nakamura
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka 819-0395, Japan.
authors.forename: Kenta
authors.initials: K
authors.lastname: Watanabe
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka 819-0395, Japan.
authors.forename: Takanori
authors.initials: T
authors.lastname: Noguchi
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka 819-0395, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Minamihata
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka 819-0395, Japan and Division of Biotechnology, Center for Future Chemistry, Kyushu University, Fukuoka 819-0395, Japan.
authors.forename: Noriho
authors.initials: N
authors.lastname: Kamiya
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka 819-0395, Japan and Center for Molecular Systems (CMS), Kyushu University, Fukuoka 819-0395, Japan.
authors.forename: Nobuo
authors.initials: N
authors.lastname: Kimizuka
mesh_terms: D036022:Capsid Proteins / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000737:chemistry*; D006639:Histidine / Q000737:chemistry*; D009532:Nickel / Q000737:chemistry*; D009571:Nitrilotriacetic Acid / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D036022:Capsid Proteins; D010455:Peptides; D049452:Green Fluorescent Proteins; D006639:Histidine; D009532:Nickel; D009571:Nitrilotriacetic Acid