title: Fractalkine restores the decreased expression of StAR and progesterone in granulosa cells from patients with polycystic ovary syndrome.
abstract: Low progesterone levels are associated with luteal phase deficiency in women with polycystic ovary syndrome (PCOS). The mechanisms regulating progesterone biosynthesis in the granulosa cells from women with PCOS is largely unknown. Fractalkine is expressed in human ovaries, and is reported to regulate progesterone production in granulosa cells of healthy women. In the current study, we aimed to examine the role of fractalkine in women with PCOS. Reduced fractalkine levels were found in follicular fluid and granulosa cells, accompanied by decreased progesterone production and reduced steroidogenic acute regulatory protein (StAR) expression in the granulosa cells of patients with PCOS. Administration of fractalkine reversed the inhibition of progesterone and StAR expression. The mechanism mediating these effects may be associated with the inhibition of ERK activity in the granulosa cells from women with PCOS. Our findings revealed that fractalkine regulated steroidogenesis in follicular granulosa cells of women with PCOS.
authors.affiliation: Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China.|Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing, China.|Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing, China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Huang
authors.affiliation: Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China.|Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing, China.|Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing, China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Pang
authors.affiliation: Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China.|Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing, China.|Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yan
authors.affiliation: Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China.|Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing, China.|Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing, China.
authors.forename: Shengli
authors.initials: S
authors.lastname: Lin
authors.affiliation: Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China.|Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing, China.|Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China.|Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing, China.|Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Lei
authors.affiliation: Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China.|Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing, China.|Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing, China.
authors.forename: Liying
authors.initials: L
authors.lastname: Yan
authors.affiliation: Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China.|Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing, China.|Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Li
authors.affiliation: Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China.|Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing, China.|Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing, China.
authors.forename: Caihong
authors.initials: C
authors.lastname: Ma
authors.affiliation: Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China.|Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing, China.|Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Qiao
mesh_terms: D000328:Adult; D015153:Blotting, Western; D054428:Chemokine CX3CL1 / Q000032:analysis* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006107:Granulosa Cells / Q000737:chemistry* / Q000187:drug effects; D006801:Humans; D010750:Phosphoproteins / Q000032:analysis*; D011085:Polycystic Ovary Syndrome / Q000473:pathology*; D011374:Progesterone / Q000032:analysis*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000098682:Steroidogenic Acute Regulatory Protein
chemical_list: C515538:CX3CL1 protein, human; D054428:Chemokine CX3CL1; D010750:Phosphoproteins; D000098682:Steroidogenic Acute Regulatory Protein; D011374:Progesterone