title: Radioprotective and Apoptotic Properties of a Combination of α-Tocopherol Acetate and Ascorbic Acid.
abstract: We studied radioprotective and apoptotic properties of a combination of α-tocopherol acetate and ascorbic acid. α-Tocopherol acetate (10 mg/kg body weight) or ascorbic acid (20 mg/kg) or combination of these agents in the same doses was orally administered to male rats at various terms before and after single whole-body exposure to γ-irradiation in the doses of 2 and 8 Gy. Irradiation increased the frequency of chromosome aberrations in bone marrow cells and plasma level of low-molecular-weight DNA. Vitamin combination administered before or after irradiation significantly reduced the frequency of chromosome aberrations by 2-2.5 times. Administration of this combination 10 min before irradiation 1.5-fold increased the content of low-molecular-weight DNA in blood plasma in comparison with the control animals exposed to radiation. The combination of α-tocopherol acetate and ascorbic acid produced radioprotective effects and enhanced apoptosis in irradiated cells.
authors.affiliation: Laboratory of Chemoprevention of Cancer and Oncopharmacology, N. N. Petrov Institute of Oncology, Ministry of the Healthcare of the Russian Federation, St. Petersburg, Russia. iravasilyeva@hotmail.com.|International Scientific Center Biotechnologies of the Third Millennium, University of Informational Technologies, Mechanics and Optics, St. Petersburg, Russia. iravasilyeva@hotmail.com.
authors.forename: I N
authors.initials: IN
authors.lastname: Vasil'eva
authors.affiliation: Laboratory of Chemoprevention of Cancer and Oncopharmacology, N. N. Petrov Institute of Oncology, Ministry of the Healthcare of the Russian Federation, St. Petersburg, Russia.|International Scientific Center Biotechnologies of the Third Millennium, University of Informational Technologies, Mechanics and Optics, St. Petersburg, Russia.
authors.forename: V G
authors.initials: VG
authors.lastname: Bespalov
authors.affiliation: International Scientific Center Biotechnologies of the Third Millennium, University of Informational Technologies, Mechanics and Optics, St. Petersburg, Russia.
authors.forename: D A
authors.initials: DA
authors.lastname: Baranenko
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001205:Ascorbic Acid / Q000494:pharmacology* / Q000627:therapeutic use; D001853:Bone Marrow / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects; D004247:DNA / Q000097:blood; D004353:Drug Evaluation, Preclinical; D004359:Drug Therapy, Combination; D008297:Male; D011833:Radiation Injuries, Experimental / Q000097:blood / Q000517:prevention & control*; D011837:Radiation-Protective Agents / Q000494:pharmacology* / Q000627:therapeutic use; D017208:Rats, Wistar; D024502:alpha-Tocopherol / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D011837:Radiation-Protective Agents; D004247:DNA; D024502:alpha-Tocopherol; D001205:Ascorbic Acid
keywords: apoptosis; ascorbic acid; low-molecularweight DNA; radioprotective substances; α-tocopherol acetate