title: Spectroscopic study of 3-Hydroxyflavone - protein interaction in lipidic bi-layers immobilized on silver nanoparticles.
abstract: The interaction of 3-Hydroxyflavone with serum proteins (BSA and HSA) in lecithin lipidic bi-layers (PC) immobilized on silver nanoparticles (SNPs), was studied by fluorescence and Raman spectroscopy. BSA secondary structure was quantified with a deconvolution algorithm, showing a decrease in α-helix structure when lipids were added to the solution. The effect of temperature on the rate of the excited-state intra-molecular proton transfer and on the dual fluorescence emission of 3-HF in the HSA/PC/SNPs systems was discussed. Evaluation of the antioxidant activity of 3-HF in HSA/PC/SNPs systems was also studied. The antioxidant activity of 3-HF decreased in the presence of SNPs. The results are discussed with relevance to the secondary structure of proteins and of the 3-HF based nano-systems to a topical formulation useful in the oxidative stress process.
authors.affiliation: Romanian Academy, Institute of Physical Chemistry "Ilie Murgulescu", Splaiul Independentei 202, 060021 Bucharest, Romania. Electronic address: voicescu@icf.ro.
authors.forename: Mariana
authors.initials: M
authors.lastname: Voicescu
authors.affiliation: Department of Physical Chemistry, University of Bucharest, Bd Regina Elisabeta 4-12, Bucharest 030018, Romania.
authors.forename: Sorana
authors.initials: S
authors.lastname: Ionescu
authors.affiliation: Polymer Department, National R&D Institute for Chemistry and Petrochemistry ICECHIM, Splaiul Independentei 202, 060021 Bucharest, Romania.
authors.forename: Cristina L
authors.initials: CL
authors.lastname: Nistor
mesh_terms: D000975:Antioxidants / Q000737:chemistry; D000067493:Dynamic Light Scattering; D005419:Flavonoids / Q000737:chemistry*; D006801:Humans; D054709:Lecithins / Q000737:chemistry; D008055:Lipids / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D017433:Protein Structure, Secondary; D012710:Serum Albumin, Bovine / Q000737:chemistry; D012834:Silver / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013059:Spectrum Analysis, Raman; D013696:Temperature
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D054709:Lecithins; D008055:Lipids; D012710:Serum Albumin, Bovine; D012834:Silver; C041477:3-hydroxyflavone
keywords: Antioxidant activity; Flavonoid; Protein structure; Silver nanoparticles; Spectroscopy