title: Inhibitory effect of 2-methyl-naphtho[1,2,3-de]quinolin-8-one on melanosome transport and skin pigmentation.
abstract: Melanosomes are lysosome-related organelles with specialized capabilities of melanin synthesis and movement mediated by the Rab27a-Melanophilin-MyosinVa protein complex. In this study, we found that 2-methyl-naphtho[1,2,3-de]quinolin-8-one (MNQO) induced melanosome aggregation around the nucleus in melan-a melanocytes and in melan-a melanocytes/SP-1 keratinocyte co-cultures without inducing toxicity or changing the melanin content. Western blot and real-time PCR analyses showed that MNQO decreased expression of the Rab27a, Melanophilin and MyosinVa proteins and mRNAs, respectively, in melan-a melanocytes. In a reconstituted human epidermis model, treatment with 0.001% MNQO reduced skin pigmentation. Also, MNQO reduced skin pigmentation in brown guinea pigs induced by UVB irradiation. These results indicated that regulation of melanosome transport may serve as a good target for new skin depigmenting agents and MNQO itself could be a candidate.
authors.affiliation: Department of Genetic engineering &Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Gyeonggi-do 446-701, Republic of Korea.
authors.forename: Jong Il
authors.initials: JI
authors.lastname: Park
authors.affiliation: Department of Genetic engineering &Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Gyeonggi-do 446-701, Republic of Korea.
authors.forename: Ha Yeon
authors.initials: HY
authors.lastname: Lee
authors.affiliation: Department of Genetic engineering &Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Gyeonggi-do 446-701, Republic of Korea.
authors.forename: Ji Eun
authors.initials: JE
authors.lastname: Lee
authors.affiliation: Department of Genetic engineering &Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Gyeonggi-do 446-701, Republic of Korea.
authors.forename: Cheol Hwan
authors.initials: CH
authors.lastname: Myung
authors.affiliation: Department of Genetic engineering &Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Gyeonggi-do 446-701, Republic of Korea.
authors.forename: Jae Sung
authors.initials: JS
authors.lastname: Hwang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001692:Biological Transport; D002449:Cell Aggregation; D002452:Cell Count; D002467:Cell Nucleus / Q000378:metabolism; D018920:Coculture Techniques; D006168:Guinea Pigs; D006801:Humans; D058965:MART-1 Antigen / Q000378:metabolism; D008543:Melanins / Q000096:biosynthesis; D008544:Melanocytes / Q000378:metabolism / Q000528:radiation effects; D020460:Melanosomes / Q000378:metabolism* / Q000528:radiation effects; D008810:Mice, Inbred C57BL; D008954:Models, Biological; D024701:Myosin Type V / Q000235:genetics / Q000378:metabolism; D009281:Naphthalenes / Q000737:chemistry / Q000378:metabolism*; D011804:Quinolines / Q000737:chemistry / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012880:Skin Pigmentation / Q000528:radiation effects; D014466:Ultraviolet Rays; D000075745:rab27 GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: C000627343:2-methyl-naphtho(1,2,3-de)quinolin-8-one; D048868:Adaptor Proteins, Signal Transducing; D058965:MART-1 Antigen; C492410:MLPH protein, human; D008543:Melanins; D009281:Naphthalenes; D011804:Quinolines; D012333:RNA, Messenger; D000075745:rab27 GTP-Binding Proteins; D024701:Myosin Type V