title: Expression of Angiotensin II Types 1 and 2 Receptors in Endometriotic Lesions.
abstract: OBJECTIVE
  The aim of this study was to evaluate the gene and protein expression of angiotensin type (AT) 1, AT2 receptors in endometriotic lesions and its relation to prostaglandin (PG) synthases.
  
  
  METHODS
  Endometriosis samples were obtained from 32 patients with endometriotic cysts. Endometrial tissues were obtained during operations for benign gynecological conditions. The expression of the AT1 and AT2 receptor mRNA and that of PG-endoperoxide synthase 2 and microsomal PGE2 synthase-1 (mPGES-1) was examined by quantitative RT-PCR. Immunohistochemical staining was performed for these receptors.
  
  
  RESULTS
  AT1 and AT2 receptor proteins were mostly located in endometrial glandular epithelium and some stromal cells. Immunoreactivity of the receptor proteins was observed in both the eutopic endometrium and endometriotic lesions. The AT1/AT2 ratio in endometriotic cysts (median 7.29, range 1.88-187.60) was significantly increased compared with that in the eutopic endometrium in the proliferative-phase in controls (median 1.01, range 0.37-2.09, p < 0.001). There was a relationship between the AT1 mRNA expression and that of mPGES-1 mRNA in the endometriotic cysts (r = 0.394089, p < 0.05). There was a significant relationship between the mRNA expression of the AT2 receptor and that of mPGES-1 in eutopic endometrium of non-endometriotic control (r = 0.610714, p < 0.05).
  
  
  CONCLUSIONS
  Renin-angiotensin system may play an important role in the pathophysiology of endometriosis.
authors.affiliation: Department of Obstetrics and Gynecology, Nihon University School of Medicine, Tokyo, Japan.
authors.forename: Takehiro
authors.initials: T
authors.lastname: Nakao
authors.forename: Fumihisa
authors.initials: F
authors.lastname: Chishima
authors.forename: Masahiko
authors.initials: M
authors.lastname: Sugitani
authors.forename: Ryusuke
authors.initials: R
authors.lastname: Tsujimura
authors.forename: Chuyu
authors.initials: C
authors.lastname: Hayashi
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Yamamoto
mesh_terms: D000328:Adult; D000804:Angiotensin II; D051546:Cyclooxygenase 2 / Q000235:genetics; D004715:Endometriosis / Q000378:metabolism* / Q000473:pathology; D004717:Endometrium / Q000737:chemistry* / Q000378:metabolism* / Q000473:pathology; D004848:Epithelium / Q000737:chemistry; D005260:Female; D015870:Gene Expression; D006801:Humans; D012333:RNA, Messenger / Q000032:analysis; D044140:Receptor, Angiotensin, Type 1 / Q000032:analysis* / Q000235:genetics; D044139:Receptor, Angiotensin, Type 2 / Q000032:analysis* / Q000235:genetics; D017154:Stromal Cells / Q000737:chemistry
chemical_list: D012333:RNA, Messenger; D044140:Receptor, Angiotensin, Type 1; D044139:Receptor, Angiotensin, Type 2; D000804:Angiotensin II; D051546:Cyclooxygenase 2
keywords: Angiotensin II receptors; Endometriosis; Microsomal prostaglandin E synthase; Prostaglandin-endoperoxide synthase