title: Mitotic genes are transcriptionally upregulated in the fibroblast irradiated with very low doses of UV-C.
abstract: Ultraviolet (UV) radiation induces a variety of biological effects, including DNA damage response and cell signaling pathways. We performed transcriptome analysis using microarray in human primary cultured fibroblasts irradiated with UV-C (0.5 or 5 J/m(2)) and harvested at 4 or 12 h following UV exposure. All transcript data were analyzed by comparison with the corresponding results in non-irradiated (control) cells. The number of genes with significantly altered expression (≥2-fold difference relative to the control) is higher in the sample irradiated with high dose of UV, suggesting that gene expression was UV dose-dependent. Pathway analysis on the upregulated genes at 12 h indicates that the expression of some cell cycle-related genes was predominantly induced irrespective of UV-dose. Interestingly, almost all the genes with significant altered expression were cell cycle-related genes designated as 'Mitotic Genes', which function in the spindle assembly checkpoint. Therefore, even a low dose of UV could affect the transcriptional profile.
authors.affiliation: Division of Dermatology, Department of Internal Related, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuou-ku, Kobe 650-0017, Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Takeuchi
authors.affiliation: Kindai University Atomic Energy Research Institute, 3-4-1 Kowakae, Higashiosaka City, Osaka 577-8502, Japan.
authors.forename: Toshiro
authors.initials: T
authors.lastname: Matsuda
authors.affiliation: Division of Dermatology, Department of Internal Related, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuou-ku, Kobe 650-0017, Japan.
authors.forename: Ryusuke
authors.initials: R
authors.lastname: Ono
authors.affiliation: Division of Dermatology, Department of Internal Related, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuou-ku, Kobe 650-0017, Japan.
authors.forename: Mariko
authors.initials: M
authors.lastname: Tsujimoto
authors.affiliation: Division of Dermatology, Department of Internal Related, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuou-ku, Kobe 650-0017, Japan.
authors.forename: Chikako
authors.initials: C
authors.lastname: Nishigori
mesh_terms: D018797:Cell Cycle Proteins / Q000235:genetics*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000502:physiology* / Q000528:radiation effects*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000528:radiation effects*; D006801:Humans; D046228:Microarray Analysis; D008938:Mitosis / Q000528:radiation effects*; D014466:Ultraviolet Rays; D015854:Up-Regulation
chemical_list: D018797:Cell Cycle Proteins