title: Identification of active aerobic methanotrophs in plateau wetlands using DNA stable isotope probing.
abstract: Sedge-dominated wetlands on the Qinghai-Tibetan Plateau are methane emission centers. Methanotrophs at these sites play a role in reducing methane emissions, but relatively little is known about the composition of active methanotrophs in these wetlands. Here, we used DNA stable isotope probing to identify the key active aerobic methanotrophs in three sedge-dominated wetlands on the plateau. We found that Methylocystis species were active in two peatlands, Hongyuan and Dangxiong. Methylobacter species were found to be active only in Dangxiong peat. Hongyuan peat had the highest methane oxidation rate, and cross-feeding of carbon from methanotrophs to methylotrophic Hyphomicrobium species was observed. Owing to a low methane oxidation rate during the incubation, the labeling of methanotrophs in Maduo wetland samples was not detected. Our results indicate that there are large differences in the activity of methanotrophs in the wetlands of this region.
authors.affiliation: School of Geography Science Nanjing Normal University, Nanjing 210023, China Department of Biogeochemistry, Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch Straße 10, 35043 Marburg, Germany dengyongcui@njnu.edu.cn.
authors.forename: Yongcui
authors.initials: Y
authors.lastname: Deng
authors.affiliation: College of Life Sciences University of the Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Cui
authors.affiliation: Department of Biogeochemistry, Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch Straße 10, 35043 Marburg, Germany.
authors.forename: Marc G
authors.initials: MG
authors.lastname: Dumont
mesh_terms: D000332:Aerobiosis; D004269:DNA, Bacterial / Q000737:chemistry*; D007553:Isotope Labeling; D008697:Methane / Q000378:metabolism*; D042082:Methylobacteriaceae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D042083:Methylocystaceae / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S; D017422:Sequence Analysis, DNA; D012987:Soil; D012988:Soil Microbiology; D053833:Wetlands
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012987:Soil; D008697:Methane
keywords: SIP; active methanotrophs; pmoA; wetland