title: [Intraperitoneal transplantation of acellular extracellular matrix-derived biomaterial has no response to innate immunity in mice].
abstract: Objective To explore the effect of acellular extracellular matrix (ECM) transplantation on the innate immunity (including macrophages and neutrophils) of mice, and establish a method evaluating the immunogenicity of acellular ECM material in mice as experimental animals. Methods Fresh membrane material and newly-processed acellular ECM material without DNA were separately intraperitoneally transplanted into mice. Seven days later, samples were collected to measure spleen index. The absolute numbers of neutrophils in peripheral blood and macrophages in peritoneal fluid were determined by BD Trucount tubes. The concentrations of interleukin 2 (IL-2), IL-4, IL-6, interferon-γ (IFN-γ), tumor necrosis factor α (TNF-α), IL-17A and IL-10 in the plasma and peritoneal fluid were detected by BD(TM) Cytometric Bead Array (CBA) mouse Th1/Th2/Th17 cytokine kit. Results Compared with sham group, the body mass is not increased in the mice transplanted with fresh membrane material, but the spleen index increased. The fresh material could dramatically increase the numbers of neutrophils in peripheral blood and macrophages in peritoneal fluid, and increase IL-6 and IFN-γ production. However, acellular ECM material prepared by improved technique only had minor or even no effect on those parameters. Conclusion Acellular ECM material with DNA removed has no obvious response to the inherent immunity in mice.
authors.affiliation: Institute of Tissue Transplantation and Immunology, College of Life Science and Technology, Jinan University, Guangzhou 510632; Grandhope Biotech Co., Ltd, National Engineering Laboratory for Regenerative Implantable Medical Devices, Guangzhou 510530, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Xu
authors.affiliation: Institute of Tissue Transplantation and Immunology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
authors.forename: Xiuyan
authors.initials: X
authors.lastname: Huang
authors.affiliation: Grandhope Biotech Co., Ltd, National Engineering Laboratory for Regenerative Implantable Medical Devices, Guangzhou 510530, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Wei
authors.affiliation: Institute of Tissue Transplantation and Immunology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China. *Corresponding author, E-mail: tzengyy@jnu.edu.cn.
authors.forename: Yaoying
authors.initials: Y
authors.lastname: Zeng
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage / Q000494:pharmacology*; D001826:Body Fluids / Q000187:drug effects / Q000378:metabolism; D016207:Cytokines / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D005109:Extracellular Matrix / Q000637:transplantation*; D005434:Flow Cytometry; D007113:Immunity, Innate / Q000187:drug effects*; D007274:Injections, Intraperitoneal; D007371:Interferon-gamma / Q000097:blood / Q000378:metabolism; D015850:Interleukin-6 / Q000097:blood / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D006377:T-Lymphocytes, Helper-Inducer / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors
chemical_list: D001672:Biocompatible Materials; D016207:Cytokines; D015850:Interleukin-6; D007371:Interferon-gamma