title: Cathepsin L knockdown enhances curcumin-mediated inhibition of growth, migration, and invasion of glioma cells.
abstract: Curcumin can be used to prevent and treat cancer. However, its exact underlying molecular mechanisms remain poorly understood. Cathepsin L, a lysosomal cysteine protease, is overexpressed in several cancer types. This study aimed to determine the role of cathepsin L in curcumin-mediated inhibition of growth, migration, and invasion of glioma cells. Results revealed that the activity of cathepsin L was enhanced in curcumin-treated glioma cells. Cathepsin L knockdown induced by RNA interference significantly promoted curcumin-induced cytotoxicity, apoptosis, and cell cycle arrest. The knockdown also inhibited the migration and invasion of glioma cells. Our results suggested that the inhibition of cathepsin L can enhance the sensitivity of glioma cells to curcumin. Therefore, cathepsin L may be a new target to enhance the efficacy of curcumin against cancers.
authors.affiliation: Department of Pharmacology, College of Pharmaceutical Sciences, Soochow University, Suzhou, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Fei
authors.affiliation: Department of Pharmacology, College of Pharmaceutical Sciences, Soochow University, Suzhou, China.
authors.forename: Yajie
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: Department of Pharmacology, College of Pharmaceutical Sciences, Soochow University, Suzhou, China.
authors.forename: Yifan
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Pharmacology, College of Pharmaceutical Sciences, Soochow University, Suzhou, China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, College of Pharmaceutical Sciences, Soochow University, Suzhou, China.
authors.forename: Meilin
authors.initials: M
authors.lastname: Han
authors.affiliation: Department of Pharmacology, College of Pharmaceutical Sciences, Soochow University, Suzhou, China.
authors.forename: Long
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, College of Pharmaceutical Sciences, Soochow University, Suzhou, China; Department of Pharmacy, Affiliated Hospital of Jiangsu University, Zhenjiang, China.
authors.forename: Caihong
authors.initials: C
authors.lastname: Tan
authors.affiliation: Department of Pharmacology, College of Pharmaceutical Sciences, Soochow University, Suzhou, China. Electronic address: liangzhongqin@suda.edu.cn.
authors.forename: Zhongqin
authors.initials: Z
authors.lastname: Liang
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001932:Brain Neoplasms / Q000188:drug therapy* / Q000201:enzymology* / Q000473:pathology / Q000503:physiopathology; D056668:Cathepsin L / Q000235:genetics / Q000378:metabolism*; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003474:Curcumin / Q000494:pharmacology* / Q000627:therapeutic use; D055785:Gene Knockdown Techniques; D005910:Glioma / Q000188:drug therapy* / Q000201:enzymology* / Q000473:pathology / Q000503:physiopathology; D006801:Humans; D009361:Neoplasm Invasiveness; D015854:Up-Regulation / Q000187:drug effects
chemical_list: C534281:CTSL protein, human; D056668:Cathepsin L; D003474:Curcumin
keywords: Apoptosis; Cathepsin L; Cell cycle arrest; Curcumin; Glioma cell; Invasion and migration