title: Phytochemical screening, antiglycation and antioxidant activities of whole plant of Boerhavia repens L. from Cholistan, Pakistan.
abstract: Present study was aimed to explore a traditionally used indigenous medicinal plant Boerhavia repens (Nyctaginaceae family) of the Cholistan desert, Pakistan. Crude aqueous and methanolic extracts of the whole plant were investigated in vitro for preliminary phytochemical screening, antioxidant and antiglycation activities. Antioxidant activities were determined by total phenolic contents, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging and inhibition of lipid peroxidation. For antiglycation activities browning production was noted and thiobarbituric acid (TBA) technique was used to determine glycation level. Boerhavia repens expressed considerable amounts of phytochemicals. Extract yield was found to be 4.59%-7.85% g/100g of dry matter with total phenolics ranging from 47.9- 190.77mg/GAE per g for aqueous and methanol extract respectively. Strong inhibitory effect was exhibited by methanolic extract in linoleic acid per oxidation system (86.11%, EC50=0.99mg/mL) and DPPH assay (88.65%, EC50=212.33μg/ml). In term of browning maximum inhibition (81.50%) was exhibited by methanolic extract at 37°C at third week of incubation. Both extracts expressed significant (P>0.05) and comparable inhibition of glycation level. In conclusion, Boerhavia repens showed promising antioxidant and antiglycation activities validating its therapeutic potential.
authors.affiliation: Department of Biochemistry and University of Agriculture, Faisalabad, Pakistan.
authors.forename: Fariha
authors.initials: F
authors.lastname: Nazneen
authors.affiliation: Department of Biochemistry and University of Agriculture, Faisalabad, Pakistan.
authors.forename: Munir A
authors.initials: MA
authors.lastname: Sheikh
authors.affiliation: Department of Biochemistry and University of Agriculture, Faisalabad, Pakistan.
authors.forename: Amir
authors.initials: A
authors.lastname: Jameel
authors.affiliation: Institute of Pharmacy, Physiology and Pharmacology, University of Agriculture, Faisalabad, Pakistan.
authors.forename: Ziaur
authors.initials: Z
authors.lastname: Rahman
mesh_terms: D001713:Biphenyl Compounds / Q000737:chemistry; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017127:Glycation End Products, Advanced / Q000378:metabolism*; D006801:Humans; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects*; D000432:Methanol / Q000737:chemistry; D039281:Nyctaginaceae / Q000737:chemistry; D018384:Oxidative Stress / Q000187:drug effects*; D010154:Pakistan; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008517:Phytotherapy; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D012709:Serum Albumin / Q000378:metabolism*; D000075462:Serum Albumin, Human; D012997:Solvents / Q000737:chemistry
chemical_list: D001713:Biphenyl Compounds; D016166:Free Radical Scavengers; D017127:Glycation End Products, Advanced; D007004:Hypoglycemic Agents; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D012709:Serum Albumin; D012997:Solvents; C109748:advanced glycation end products-human serum albumin; C004931:1,1-diphenyl-2-picrylhydrazyl; D000432:Methanol; D000075462:Serum Albumin, Human