title: Helicobacter pylori Peptidyl Prolyl Isomerase Expression Is Associated with the Severity of Gastritis.
abstract: OBJECTIVE
  Helicobacter pylori secretory peptidyl prolyl isomerase, HP0175, is progressively identified as a pro-inflammatory and pro-carcinogenic protein, which serves to link H. pylori infection to its more severe clinical outcomes. Here, we have analyzed host HP0175-specific antibody responses in relation to the severity of gastritis.
  
  
  METHODS
  The HP0175 gene fragment was PCR-amplified, cloned, expressed and purified by Ni-NTA affinity chromatography. Serum antigen-specific antibody responses of non-ulcer dyspeptic patients (N = 176) against recombinant HP0175 were detected by western blotting. The infection status of these subjects was determined by rapid urease test, culture, histology, and serology. The grade of inflammation and stage of atrophy were scored blindly according to the OLGA staging system.
  
  
  RESULTS
  The recombinant HP0175 (rHP0175) was expressed as a ~35 kDa protein and its identity was confirmed by western blotting using anti-6X His tag antibody and pooled H. pylori-positive sera. Serum IgG antibodies against rHP0175 segregated our patients into two similar-sized groups of sero-positives (90/176, 51.1 %) and sero-negatives (86/176, 48.9 %). The former presented with higher grades of gastric inflammation (OR = 4.4, 95 % CI = 1.9-9.9, P = 0.001) and stages of gastric atrophy (OR = 18.3, 95 %CI = 1.4-246.6, P = 0.028).
  
  
  CONCLUSIONS
  Our findings lend further support to the pro-inflammatory nature of H. pylori peptidyl prolyl isomerase (HP0175) and recommends this antigen as a non-invasive serum biomarker of the severity of H. pylori-associated gastritis.
authors.affiliation: HPGC Group, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, 1316943551, Iran.
authors.forename: Akbar
authors.initials: A
authors.lastname: Oghalaie
authors.affiliation: HPGC Group, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, 1316943551, Iran.
authors.forename: Samaneh
authors.initials: S
authors.lastname: Saberi
authors.affiliation: HPGC Group, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, 1316943551, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Esmaeili
authors.affiliation: HPGC Group, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, 1316943551, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Ebrahimzadeh
authors.affiliation: Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, 1316943551, Iran.
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Barkhordari
authors.affiliation: Fungal Biotechnology Lab, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, 1316943551, Iran.
authors.forename: Abdolreza
authors.initials: A
authors.lastname: Ghamarian
authors.affiliation: Department of Gastroenterology, Amiralam Hospital, Tehran University of Medical Sciences, Tehran, 1145765111, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Tashakoripoor
authors.affiliation: Cancer Institute, Tehran University of Medical Sciences, Tehran, 141979733141, Iran.
authors.forename: Afshin
authors.initials: A
authors.lastname: Abdirad
authors.affiliation: Department of Gastroenterology, Amiralam Hospital, Tehran University of Medical Sciences, Tehran, 1145765111, Iran.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Eshagh Hosseini
authors.affiliation: Fungal Biotechnology Lab, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, 1316943551, Iran.
authors.forename: Vahid
authors.initials: V
authors.lastname: Khalaj
authors.affiliation: HPGC Group, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, 1316943551, Iran. marjan.mohammadi@pasteur.ac.ir.
authors.forename: Marjan
authors.initials: M
authors.lastname: Mohammadi
mesh_terms: D005260:Female; D005756:Gastritis / Q000473:pathology / Q000821:virology*; D016480:Helicobacter pylori / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D019696:Peptidylprolyl Isomerase / Q000378:metabolism*
chemical_list: D019696:Peptidylprolyl Isomerase
keywords: Atrophy; Biomarker; HP0175; Inflammation; PPIase