title: Generation and characterization of tamoxifen-inducible Pax9-CreER knock-in mice using CrispR/Cas9.
abstract: Pax9 encodes a paired-box homeodomain (Pax) transcription factor and is critical for the development of multiple organs. Using CrispR/Cas9-mediated homologous directed repair (HDR), we generated a new Pax9-CreER knock-in mouse line in which the CreER(T2) fusion protein is produced after synthesis of endogenous Pax9 protein. We found that tdTomato reporter expression in Pax9-CreER;tdTomato reporter mice is detectable in a similar pattern to the endogenous Pax9 expression, faithfully recapitulating the Pax9 expression domains throughout the embryo and in the adult mouse. At early embryonic stages, the tdTomato reporter is expressed first in the pharyngeal pouch region and later in the craniofacial mesenchyme, somites, limbs, and lingual papillae in the adult tongue. These results demonstrate that this new Pax9-CreER knock-in mouse line can be used for lineage tracing and genetic targeting of Pax9-expressing cells and their progeny in a temporally and spatially controlled manner during development and organogenesis.
authors.affiliation: Center for Craniofacial Molecular Biology, University of Southern California, Los Angeles, CA, 90033, USA.
authors.forename: Jifan
authors.initials: J
authors.lastname: Feng
authors.affiliation: Center for Craniofacial Molecular Biology, University of Southern California, Los Angeles, CA, 90033, USA.|State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Junjun
authors.initials: J
authors.lastname: Jing
authors.affiliation: Center for Craniofacial Molecular Biology, University of Southern California, Los Angeles, CA, 90033, USA.|Center for Personalized Medicine, Children's Hospital Los Angeles, Los Angeles, CA, 90027, USA.|Department of Pathology & Pediatrics, Keck School of Medicine, University of Southern California, Los Angeles, CA, 90089, USA.
authors.forename: Pedro A
authors.initials: PA
authors.lastname: Sanchez-Lara
authors.affiliation: Center for Personalized Medicine, Children's Hospital Los Angeles, Los Angeles, CA, 90027, USA.
authors.forename: Moiz S
authors.initials: MS
authors.lastname: Bootwalla
authors.affiliation: Center for Personalized Medicine, Children's Hospital Los Angeles, Los Angeles, CA, 90027, USA.|Department of Preventive Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA, 90089, USA.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Buckley
authors.affiliation: USC Norris Comprehensive Cancer Center Transgenic/Knockout Rodent Core Facility, Los Angeles, CA, 90089, USA.
authors.forename: Nancy
authors.initials: N
authors.lastname: Wu
authors.affiliation: USC Norris Comprehensive Cancer Center Transgenic/Knockout Rodent Core Facility, Los Angeles, CA, 90089, USA.
authors.forename: Youzhen
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Center for Craniofacial Molecular Biology, University of Southern California, Los Angeles, CA, 90033, USA. ychai@usc.edu.
authors.forename: Yang
authors.initials: Y
authors.lastname: Chai
mesh_terms: D000818:Animals; D064113:CRISPR-Cas Systems; D055879:Gene Knock-In Techniques / Q000379:methods*; D019426:Integrases / Q000235:genetics; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D051764:PAX9 Transcription Factor / Q000235:genetics; D013629:Tamoxifen / Q000494:pharmacology; D015533:Transcriptional Activation / Q000187:drug effects; D000097573:Red Fluorescent Protein
chemical_list: D019426:Integrases; D008164:Luminescent Proteins; D051764:PAX9 Transcription Factor; D013629:Tamoxifen; D000097573:Red Fluorescent Protein; C045073:Cre recombinase
keywords: Pax9; Pax9-CreER; genetic labeling; mouse development