title: A Sensitive and Rapid Method for Mapping Protein Bound to DNA by Atomic Force Microscopy.
abstract: We addressed the strategies of mapping protein binding sites on a DNA fragment by atomic force microscopy (AFM). The protein binding site was uniquely mapped by distinguishing two termini of a linear DNA fragment. Our simple methods were found very useful to get information on transcription regulatory regions by taking advantage of long range and quick scanning by AFM.
authors.affiliation: a Joint Research Center for Atom Technology , Higashi 1-1-4, Tsukuba, Ibaraki 305 , Japan.
authors.forename: M
authors.initials: M
authors.lastname: Tanigawa
authors.affiliation: a Joint Research Center for Atom Technology , Higashi 1-1-4, Tsukuba, Ibaraki 305 , Japan.|b Department of Molecular Biology , National Institute of Bioscience and Human-Technology , Higashi 1-1, Tsukuba, Ibaraki 305 , Japan.
authors.forename: M
authors.initials: M
authors.lastname: Machida
authors.affiliation: a Joint Research Center for Atom Technology , Higashi 1-1-4, Tsukuba, Ibaraki 305 , Japan.
authors.forename: T
authors.initials: T
authors.lastname: Okada
keywords: DNA mapping; DNA-protein complex; Spl; atomic force microscopy