title: The effects of supplemental melatonin administration on the healing of bone defects in streptozotocin-induced diabetic rats.
abstract: OBJECTIVE
  The aim of this study was to investigate histologic and biochemical effects of supplemental melatonin administration on bone healing and antioxidant defense mechanism in diabetic rats.
  
  
  METHODS
  Eighty-six Sprague-Dawley male rats were used in this study. Diabetes mellitus was induced by intraperitoneal (i.p.) administration of 65 mg/kg streptozotocin (STZ). Surgical bone defects were prepared in the tibia of each animal. Diabetic animals and those in control groups were treated either with daily melatonin (250 μg/animal/day/i.p.) diluted in ethanol, only ethanol, or sterile saline solution. Rats were humanely killed at the 10th and 30th postoperative days. Plasma levels of Advanced Oxidation Protein Products (AOPP), Malondialdehyde (MDA), and Superoxide Dismutase (SOD) were measured. The number of osteoblasts, blood vessels and the area of new mineralized tissue formation were calculated in histologic sections.
  
  
  RESULTS
  At the 10th day, DM+MEL (rats receiving both STZ and melatonin) group had significantly higher number of osteoblasts and blood vessels as well as larger new mineralized tissue surfaces (p<0.05 for each) when compared with DM group. At the 30th day, DM group treated with melatonin had significantly lower levels of AOPP and MDA than those of DM group (p<0.05).
  
  
  CONCLUSIONS
  Melatonin administration in STZ induced diabetic rats reduced oxidative stress related biomarkers and showed beneficial effects on bone healing at short term.
authors.affiliation: - Istanbul University, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Istanbul, Turkey.
authors.forename: Senem
authors.initials: S
authors.lastname: Yildirimturk
authors.affiliation: - Istanbul University, Faculty of Dentistry, Department of Biochemistry, Istanbul, Turkey.
authors.forename: Sule
authors.initials: S
authors.lastname: Batu
authors.affiliation: - Istanbul University, Institute of Oncology, Department of Pathology, Istanbul, Turkey.
authors.forename: Canan
authors.initials: C
authors.lastname: Alatli
authors.affiliation: - Istanbul University, Institute of Oncology, Department of Pathology, Istanbul, Turkey.
authors.forename: Vakur
authors.initials: V
authors.lastname: Olgac
authors.affiliation: - Istanbul University, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Istanbul, Turkey.
authors.forename: Deniz
authors.initials: D
authors.lastname: Firat
authors.affiliation: - Istanbul University, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Istanbul, Turkey.
authors.forename: Yigit
authors.initials: Y
authors.lastname: Sirin
mesh_terms: D062107:Advanced Oxidation Protein Products / Q000097:blood; D000818:Animals; D015415:Biomarkers; D002113:Calcification, Physiologic / Q000187:drug effects; D002452:Cell Count; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000378:metabolism*; D005355:Fibrosis; D017102:Fracture Healing / Q000187:drug effects*; D016166:Free Radical Scavengers / Q000008:administration & dosage*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008550:Melatonin / Q000008:administration & dosage*; D010006:Osteoblasts / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D015203:Reproducibility of Results; D013311:Streptozocin; D013482:Superoxide Dismutase / Q000097:blood; D013977:Tibia / Q000187:drug effects / Q000473:pathology; D013997:Time Factors
chemical_list: D062107:Advanced Oxidation Protein Products; D015415:Biomarkers; D016166:Free Radical Scavengers; D008315:Malondialdehyde; D013311:Streptozocin; D013482:Superoxide Dismutase; D008550:Melatonin