title: Interaction of TGA@CdTe Quantum Dots with an Extracellular Matrix of Haematococcus pluvialis Microalgae Detected Using Surface-Enhanced Raman Spectroscopy (SERS).
abstract: The present study reports the localization and interaction of thioglycolic acid (TGA) capped CdTe quantum dots (TGA@CdTe QDs) within the extracellular matrix (ECM) of Haematococcus pluvialis (Chlorophyceae) microalgae (HPM) after an incubation period of 5 min. Changes in the Raman spectrum of HPM induced by the adsorption of the TGA@CdTe QDs are successfully found by using naked gold anisotropic structures as nano-sensors for surface-enhanced Raman scattering (SERS effect). Raman spectroscopy results show that TGA@CdTe QDs interact with the biomolecules present in the ECM. Sample preparation and characterization by complementary techniques such as confocal and electron microscopy are also used to confirm the presence and localization of the nanoparticles in the algae. This research shows new evidence on early accumulation of QDs in plant cells and would further improve our understanding about their environmental impact.
authors.affiliation: Centro de Investigaciones en Óptica, León, México.
authors.forename: Elisa
authors.initials: E
authors.lastname: Cepeda-Pérez
authors.affiliation: Laboratorio de Nanotecnología Ambiental, Centro del Agua para América Latina y el Caribe, Tecnológico de Monterrey, Monterrey, México.
authors.forename: Iris
authors.initials: I
authors.lastname: Aguilar-Hernández
authors.affiliation: Centro de Investigaciones en Óptica, León, México tzarara@cio.mx.
authors.forename: Tzarara
authors.initials: T
authors.lastname: López-Luke
authors.affiliation: Centro de Investigaciones en Óptica, León, México.
authors.forename: Valeria
authors.initials: V
authors.lastname: Piazza
authors.affiliation: Centro de Investigaciones en Óptica, León, México.
authors.forename: Ramón
authors.initials: R
authors.lastname: Carriles
authors.affiliation: Laboratorio de Nanotecnología Ambiental, Centro del Agua para América Latina y el Caribe, Tecnológico de Monterrey, Monterrey, México.
authors.forename: Nancy
authors.initials: N
authors.lastname: Ornelas-Soto
authors.affiliation: Centro de Investigaciones en Óptica, León, México.
authors.forename: Elder
authors.initials: E
authors.lastname: de la Rosa
mesh_terms: D019187:Cadmium Compounds / Q000737:chemistry* / Q000378:metabolism; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry; D058086:Microalgae / Q000737:chemistry* / Q000378:metabolism; D045663:Quantum Dots / Q000737:chemistry* / Q000378:metabolism; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013691:Tellurium / Q000737:chemistry* / Q000378:metabolism; D013864:Thioglycolates / Q000737:chemistry* / Q000378:metabolism; D016823:Volvocida / Q000737:chemistry* / Q000378:metabolism
chemical_list: D019187:Cadmium Compounds; D013864:Thioglycolates; D006046:Gold; C017487:2-mercaptoacetate; D013691:Tellurium; C028337:cadmium telluride
keywords: SERS; confocal microscopy; electron microscopy; freshwayer microalgae; gold nanoparticles; haematococcus pluvialis; quantum dots