title: AAV9 Delivery of shRNA to the Mouse Heart.
abstract: RNA interference (RNAi) is a rapid approach to dissect loss-of-function phenotype for a gene of interest. However, it is challenging to perform RNAi in specific organs and tissues in vivo. Engineered viruses can provide a useful tool for delivery of small RNAs in vivo. Recombinant adeno-associated viruses (rAAVs) are the preferred method for delivering genes or gene modulators to target cells due to their high titer, low immune response, ability to transduce many types of cell, and overall safety. In this unit, we describe protocols for use of rAAVs as a cargo to deliver miRNA backbone-based shRNA controlled by a cardiac-specific promoter into the mouse heart. © 2016 by John Wiley & Sons, Inc.
authors.affiliation: Department of Genetics, Harvard Medical School, Boston, Massachusetts.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Wakimoto
authors.affiliation: Department of Genetics, Harvard Medical School, Boston, Massachusetts.
authors.forename: J G
authors.initials: JG
authors.lastname: Seidman
authors.affiliation: Cardiovascular Research Institute (CVRI), Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
authors.forename: Roger S Y
authors.initials: RSY
authors.lastname: Foo
authors.affiliation: Department of Genetics, Harvard Medical School, Boston, Massachusetts.|Cardiovascular Research Institute (CVRI), Yong Loo Lin School of Medicine, National University of Singapore, Singapore.|Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
authors.forename: Jianming
authors.initials: J
authors.lastname: Jiang
mesh_terms: D000818:Animals; D000229:Dependovirus / Q000235:genetics*; D018014:Gene Transfer Techniques; D005818:Genetic Engineering; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D009206:Myocardium / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000235:genetics; D014161:Transduction, Genetic
chemical_list: D034741:RNA, Small Interfering
keywords: AAV; cardiovascular; shRNA