title: Ultrasonic-microwave method in preparation of polypyrrole-coated magnetic particles for vitamin D extraction in milk.
abstract: In this study, a nanocomposite of polypyrrole-coated magnetite nanoparticles (Fe3O4@PPy) was prepared by ultrasonic-microwave technique, and employed as magnetic solid-phase extraction (MSPE) sorbent for extraction of vitamin D from milk samples. The term of the synthesis by ultrasonic-microwave technique was dramatically shortened within 4h compared to 20h by conventional stirring-heating method. The resultant composites incorporating the π-π bonding (between PPy coating and the analytes) and magnetic separation can be applied for vitamin D analysis in complicated samples. Without saponification or protein precipitation, vitamin D2 and vitamin D3 could be captured directly from milk samples by Fe3O4@PPy, and separated by magnetic field with only 0.5mL desorption solvent. The total preparation time was completed within 15min. A method for the determination of vitamin D in milk samples by the Fe3O4@PPy extraction coupled with high performance liquid chromatography (HPLC) was developed. The LODs of vitamin D2 and vitamin D3, based on signal-to-noise ratio (S/N) of 3, were 0.02ng/mL and 0.05ng/mL respectively. The recoveries of vitamin D2 and vitamin D3 from milk samples were in the range of 71.9-90.3%, with relative standard deviations ranging between 3.6%-9.9%. The results indicated that the Fe3O4@PPy can be favorably used for the extraction of the vitamin D in milk samples.
authors.affiliation: College of Chemistry and Environmental Engineering, Dongguan University of Technology, Dongguan 523808, China. Electronic address: jiaoz@dgut.edu.cn.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Jiao
authors.affiliation: College of Chemistry and Environmental Engineering, Dongguan University of Technology, Dongguan 523808, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Environmental Engineering, Dongguan University of Technology, Dongguan 523808, China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Fan
mesh_terms: D000818:Animals; D002762:Cholecalciferol / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004872:Ergocalciferols / Q000302:isolation & purification; D057230:Limit of Detection; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008872:Microwaves; D008892:Milk / Q000737:chemistry*; D053761:Nanocomposites; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D059629:Signal-To-Noise Ratio; D052616:Solid Phase Extraction / Q000379:methods; D000069453:Ultrasonic Waves; D014807:Vitamin D / Q000302:isolation & purification*; D014815:Vitamins / Q000302:isolation & purification*
chemical_list: D004872:Ergocalciferols; D058185:Magnetite Nanoparticles; D011108:Polymers; D011758:Pyrroles; D014815:Vitamins; D014807:Vitamin D; D002762:Cholecalciferol; C067635:polypyrrole
keywords: Fe(3)O(4)@PPy; Magnetic solid-phase extraction; Ultrasonic-microwave method; Vitamin D