title: Characterization of novel DeoR-family member from the Streptomyces ahygroscopicus strain CK-15 that acts as a repressor of morphological development.
abstract: Wuyiencin is produced by Streptomyces ahygroscopicus var. wuyiensis, which has been widely used in China as an industrially produced biopesticide to control various fungal diseases. Although its mechanism of action, breeding, and fermentation had been extensively characterized, less is known about the regulatory functions that affect its biosynthesis or morphological development. The wysR3 gene of S. ahygroscopicus strain CK-15, a novel member of the DeoR family of regulatory genes, was assessed to determine its function by gene knockdown. Herein, we demonstrate for the first time that DeoR family proteins derived from the same source are likely to be a single branch in a phylogenetic tree and show that wysR3 acts as a repressor for its morphological development without effecting wuyiencin production. We found that the ΔwysR3 strain can grow quickly to reach a plateau stage of maximum biomass at 60 h, which is ∼12 h faster than the wild-type strain. In the industrial fermentation production process, the ΔwysR3 strain can reduce consumption and save both time and money.
authors.affiliation: State Key Laboratory of Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Yuanmingyuan West Road, No. 2, Haidian District, Beijing, 100193, People's Republic of China.
authors.forename: Beibei
authors.initials: B
authors.lastname: Ge
authors.affiliation: State Key Laboratory of Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Yuanmingyuan West Road, No. 2, Haidian District, Beijing, 100193, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Yuanmingyuan West Road, No. 2, Haidian District, Beijing, 100193, People's Republic of China.
authors.forename: Binghua
authors.initials: B
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Yuanmingyuan West Road, No. 2, Haidian District, Beijing, 100193, People's Republic of China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Yuanmingyuan West Road, No. 2, Haidian District, Beijing, 100193, People's Republic of China. zhangkecheng@sina.com.
authors.forename: Kecheng
authors.initials: K
authors.lastname: Zhang
mesh_terms: D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D055785:Gene Knockdown Techniques; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism*; D013302:Streptomyces / Q000235:genetics* / Q000254:growth & development*
chemical_list: D012097:Repressor Proteins
keywords: DeoR family; Morphological development; Regulator gene; Streptomyces