title: Chemical morphology of Areca nut characterized directly by Fourier transform near-infrared and mid-infrared microspectroscopic imaging in reflection modes.
abstract: Fourier transform near-infrared (NIR) and mid-infrared (MIR) imaging techniques are essential tools to characterize the chemical morphology of plant. The transmission imaging mode is mostly used to obtain easy-to-interpret spectra with high signal-to-noise ratio. However, the native chemical compositions and physical structures of plant samples may be altered when they are microtomed for the transmission tests. For the direct characterization of thick plant samples, the combination of the reflection NIR imaging and the attenuated total reflection (ATR) MIR imaging is proposed in this research. First, the reflection NIR imaging method can explore the whole sample quickly to find out typical regions in small sizes. Next, each small typical region can be measured by the ATR-MIR imaging method to reveal the molecular structures and spatial distributions of compounds of interest. As an example, the chemical morphology of Areca nut section is characterized directly by the above approach.
authors.affiliation: Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084, China.
authors.forename: Jian-Bo
authors.initials: JB
authors.lastname: Chen
authors.affiliation: Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084, China.
authors.forename: Su-Qin
authors.initials: SQ
authors.lastname: Sun
authors.affiliation: Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Tsinghua University, Beijing 100084, China. Electronic address: zhouqun@tsinghua.edu.cn.
authors.forename: Qun
authors.initials: Q
authors.lastname: Zhou
mesh_terms: D001114:Areca / Q000737:chemistry*; D005583:Fourier Analysis; D015591:Microspectrophotometry / Q000379:methods; D015394:Molecular Structure; D009754:Nuts / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis*; D059629:Signal-To-Noise Ratio; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D019265:Spectroscopy, Near-Infrared / Q000379:methods*
chemical_list: D010936:Plant Extracts
keywords: Areca nut; Attenuated total reflection; FT-IR spectroscopic imaging; MIR spectroscopy; NIR spectroscopy