title: Microbial Community of Healthy Thai Vegetarians and Non-Vegetarians, Their Core Gut Microbiota, and Pathogen Risk.
abstract: Pyrosequencing analysis of intestinal microflora from healthy Thai vegetarians and non-vegetarians exhibited 893 OTUs covering 189 species. The strong species indicators of vegetarians and non-vegetarians werePrevotella copriandBacteroides vulgatusas well as bacteria close toEscherichia hermaniiwith % relative abundance of 16.9 and 4.5-4.7, respectively. Core gut microbiota of the vegetarian and non-vegetarian groups consisted of 11 and 20 different bacterial species, respectively, belonging to Actinobacteria, Firmicutes, and Proteobacteria commonly found in both groups. Two species,Faecalibacterium prausnitziiandGemmiger formicilis, had a prevalence of 100% in both groups. Three species,Clostridium nexile,Eubacterium eligens, andP. copri, showed up in most vegetarians, whereas more diversity ofCollinsella aerofaciens,Ruminococcus torques, various species ofBacteroides,Parabacteroides,Escherichia, and different species ofClostridiumandEubacteriumwere found in most non-vegetarians. Considering the correlation of personal characters, consumption behavior, and microbial groups, the age of non-vegetarians showed a strong positive correlation coefficient of 0.54 (p= 0.001) toBacteroides uniformisbut exhibited a moderate one toAlistipes finegoldiiandB. vulgatus. Only a positive moderate correlation of body mass index andParabacteroides distasonisappeared. Based on the significant abundance of potential pathogens, the microbiota of the non-vegetarian group showed an abundance of potential pathogen varieties ofBilophila wadsworthia,Escherichia coli, andE. hermannii, whereas that of the vegetarian group served for onlyKlebsiella pneumoniae. These results implied that the microbiota of vegetarians with high abundance ofP. copriand low potential pathogen variety would be a way to maintain good health in Thais.
authors.affiliation: Specialized Research Unit: Probiotics and Prebiotics for Health, Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.|Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.|Bioscience Department, Thailand Institute of Scientific and Technological Research, Technopolis, Pathum Thani 12120, Thailand.
authors.forename: Supatjaree
authors.initials: S
authors.lastname: Ruengsomwong
authors.affiliation: Specialized Research Unit: Probiotics and Prebiotics for Health, Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
authors.forename: Orawan
authors.initials: O
authors.lastname: La-Ongkham
authors.affiliation: Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka 812-8581, Japan.
authors.forename: Jiahui
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Bioscience Department, Thailand Institute of Scientific and Technological Research, Technopolis, Pathum Thani 12120, Thailand.
authors.forename: Bhusita
authors.initials: B
authors.lastname: Wannissorn
authors.affiliation: Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka 812-8581, Japan.
authors.forename: Jiro
authors.initials: J
authors.lastname: Nakayama
authors.affiliation: Specialized Research Unit: Probiotics and Prebiotics for Health, Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.|Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
authors.forename: Sunee
authors.initials: S
authors.lastname: Nitisinprasert
mesh_terms: D000328:Adult; D000368:Aged; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D001439:Bacteroides; D003430:Cross-Sectional Studies; D014676:Diet, Vegetarian; D005243:Feces / Q000382:microbiology*; D000069196:Gastrointestinal Microbiome; D006801:Humans; D008875:Middle Aged; D018720:Prevotella; D013785:Thailand / Q000453:epidemiology
keywords: Enterobacteriaceae; Fecal microbiota; Prevotella; core gut microbiota; pathogen risk; pyrosequencing