title: Sweetness-induced activation of membrane dipole potential in STC-1 taste cells.
abstract: The biological functions of cell membranes strongly influence the binding and transport of molecular species. We developed STC-1 cell line stably expressing the sweet taste receptor (T1R2/T1R3), and explored the possible correlation between sweeteners and membrane dipole potential of STC-1 cells. In this study, sweetener-induced dipole potential activation was elucidated using a fluorescence-based measurement technique, by monitoring the voltage sensitive probe Di-8-ANEPPS using a dual wavelength ratiometric approach. It indicated that the presence of sweeteners resulted in cell membrane dipole potential change, and interaction of artificial sweeteners with taste cells resulted in a greater reduction in potential compared with natural sweeteners. Our work presents a newly developed approach using a fluorescence-based measurement technique to study sweetener-induced dipole potential activation of STC-1 cells. This new approach could be used as a complementary tool to study the function of sweet taste receptors or other GPCRs and helps to understand the basis sweetness mechanism.
authors.affiliation: College of Food & Biology Engineering, Zhejiang Gongshang University, Hangzhou, Zhejiang 310035, China; Zhejiang Provincial Key Lab for Chem & Bio Processing Technology of Agricultural Products, Zhejiang University of Science and Technology, Hangzhou, Zhejiang 310023, China.
authors.forename: Li-Chun
authors.initials: LC
authors.lastname: Chen
authors.affiliation: College of Food & Biology Engineering, Zhejiang Gongshang University, Hangzhou, Zhejiang 310035, China.
authors.forename: Ning-Ning
authors.initials: NN
authors.lastname: Xie
authors.affiliation: College of Food & Biology Engineering, Zhejiang Gongshang University, Hangzhou, Zhejiang 310035, China. Electronic address: spdeng@mail.zjgsu.edu.cn.
authors.forename: Shao-Ping
authors.initials: SP
authors.lastname: Deng
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D019858:Enteroendocrine Cells / Q000187:drug effects / Q000502:physiology*; D005456:Fluorescent Dyes / Q000737:chemistry; D007091:Image Processing, Computer-Assisted; D008564:Membrane Potentials / Q000187:drug effects*; D051379:Mice; D011726:Pyridinium Compounds / Q000737:chemistry; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism*; D013549:Sweetening Agents / Q000737:chemistry / Q000494:pharmacology*; D013649:Taste / Q000502:physiology*; D055697:Taste Perception
chemical_list: C089669:1-(3-sulfonatopropyl)-4-(beta-(2-(di-n-octylamino)-6-naphthyl)vinyl)pyridinium betaine; D005456:Fluorescent Dyes; D011726:Pyridinium Compounds; D043562:Receptors, G-Protein-Coupled; D013549:Sweetening Agents; C431926:taste receptors, type 1
keywords: Dipole potential; Membrane; Sweeteners; Taste perception