title: Upregulation of Leukocytic Syncytin-1 in Acute Myeloid Leukemia Patients.
abstract: BACKGROUND Syncytin-1, a cell membrane-localizing fusogen, is abnormally expressed in several cancers, including endometrial cancer, breast cancer, and leukemia. Although abnormal syncytin-1 expression has been detected in two-thirds of leukemia blood samples, its expression profile in acute leukemia patients has not yet been analyzed. MATERIAL AND METHODS Bone marrow samples from 50 acute myelogenous leukemia (AML) cases and 14 B-cell acute lymphocytic leukemia (B-cell ALL) patients were subjected to flow cytometry to assess leukocyte type distributions and leukocytic syncytin-1 surface expression. RT-PCR was applied to assess leukocytic syncytin-1 mRNA expression. Statistical analysis was applied to compare syncytin-1 expression between AML and B-cell ALL patients across blasts, granulocytes, lymphocytes, and monocytes as well as to determine clinical factors statistically associated with changes in syncytin-1 expression. RESULTS The leukocyte type distributions of the AML and B-cell ALL cohorts highly overlapped, with an observable difference in blast distribution between the 2 cohorts. The AML cohort displayed significantly greater syncytin-1 surface and mRNA expression (p<0.05). Syncytin-1 surface and mRNA expression was significantly increased across all 4 leukocyte types (p<0.05). The percentage of syncytin-1-expressing blasts was significantly greater in AML patients (p<0.05), with blasts showing the largest fold-change in syncytin-1 expression (p<0.05). M5, M5a, and M5b AML patients displayed significantly higher syncytin-1 surface expression relative to all other AML French-American-British (FAB) classifications (p<0.05). CONCLUSIONS These findings suggest leukocytic syncytin-1 expression may play a role in the development and/or maintenance of the AML phenotype and the acute monocytic leukemia phenotype in particular.
authors.affiliation: Department of Clinical Laboratories, The First People's Hospital of Yunnan Province, Kunming, Yunnan, China (mainland).
authors.forename: Yi
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Clinical Laboratories, The First People's Hospital of Yunnan Province, Kunming, Yunnan, China (mainland).
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Clinical Laboratories, The First People's Hospital of Yunnan Province, Kunming, Yunnan, China (mainland).
authors.forename: Jianxin
authors.initials: J
authors.lastname: Song
authors.affiliation: Department of Clinical Laboratories, The First People's Hospital of Yunnan Province, Kunming, Yunnan, China (mainland).
authors.forename: Yaxian
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Clinical Laboratories, The First People's Hospital of Yunnan Province, Kunming, Yunnan, China (mainland).
authors.forename: Hongmei
authors.initials: H
authors.lastname: Ouyang
authors.affiliation: Department of Rehabilitation, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China (mainland).
authors.forename: Rongzhong
authors.initials: R
authors.lastname: Huang
authors.affiliation: Department of Clinical Laboratories, The First People's Hospital of Yunnan Province, Kunming, Yunnan, China (mainland).
authors.forename: Guiqian
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratories, The First People's Hospital of Yunnan Province, Kunming, Yunnan, China (mainland).
authors.forename: Xin
authors.initials: X
authors.lastname: Fan
authors.affiliation: Department of Clinical Laboratories, The First People's Hospital of Yunnan Province, Kunming, Yunnan, China (mainland).
authors.forename: Rui
authors.initials: R
authors.lastname: Tao
authors.affiliation: Department of Clinical Laboratories, The First People's Hospital of Yunnan Province, Kunming, Yunnan, China (mainland).
authors.forename: Jie
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Department of Clinical Laboratories, The First People's Hospital of Yunnan Province, Kunming, Yunnan, China (mainland).
authors.forename: Hua
authors.initials: H
authors.lastname: Niu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001402:B-Lymphocytes / Q000378:metabolism; D001853:Bone Marrow / Q000378:metabolism / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D005260:Female; D015686:Gene Products, env / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000097:blood* / Q000235:genetics / Q000473:pathology; D007958:Leukocyte Count; D007962:Leukocytes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D015452:Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / Q000097:blood* / Q000235:genetics / Q000473:pathology; D011257:Pregnancy Proteins / Q000096:biosynthesis* / Q000235:genetics; D059467:Transcriptome; D015854:Up-Regulation
chemical_list: D015686:Gene Products, env; D011257:Pregnancy Proteins; C405335:syncytin