title: Facile synthesis of red emitting 3-aminophenylboronic acid functionalized copper nanoclusters for rapid, selective and highly sensitive detection of glycoproteins.
abstract: As an emerging class of fluorescent probes, copper nanoclusters (Cu NCs) have been considered as an intriguing candidate for detecting biomoleculars due to their outstanding fluorescent properties, excellent biocompatibility and low cost. Herein, we fabricated bovine serum albumin (BSA) protected Cu NCs (BSA-Cu NCs) and further functionalized them with 3-aminophenylboronic acid (APBA) for selectively discerning glycoproteins. In aqueous solution, Cu(2+) ions were directly reduced into BSA-Cu NCs by hydrazine hydrate (N2H4·H2O) at room-temperature using BSA as the capping agent. The synthetic process was very rapid, simple and easy for controlling due to the lack of any other complicated procedure such as heating and adjusting the pH value of the reactive mixture. The APBA-Cu NCs showed strong fluorescent emission at 630nm in the red range. So it can effectively avoid the disturbance of auto-fluorescence in biosamples. The fluorescence of the APBA-Cu NCs was obviously quenched by glycoprotein samples. Then, the APBA-Cu NCs were employed as a probe for selective capture and sensitive detection of glycoproteins with a wide linear range of 5-220nM and a low detection limit of 2.60nM owing to the covalent reaction between the boric acid group of APBA and the cis-glycol groups of the glycoproteins. The developed method was also successfully applied to determine glycoproteins in egg white of chickens and human urine samples with quantitative spike recoveries from 95% to 104%.
authors.affiliation: Key Laboratory of Inorganic-Organic Hybrid Functional Material Chemistry (Tianjin Normal University), Ministry of Education, Tianjin Key Laboratory of Structure and Performance for Functional Molecule, College of Chemistry, Tianjin Normal University, 393 Binshui West Road, Tianjin 300387, PR China.
authors.forename: Xin-Ge
authors.initials: XG
authors.lastname: Li
authors.affiliation: Key Laboratory of Inorganic-Organic Hybrid Functional Material Chemistry (Tianjin Normal University), Ministry of Education, Tianjin Key Laboratory of Structure and Performance for Functional Molecule, College of Chemistry, Tianjin Normal University, 393 Binshui West Road, Tianjin 300387, PR China. Electronic address: hxxyzf@mail.tjnu.edu.cn.
authors.forename: Fei
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Inorganic-Organic Hybrid Functional Material Chemistry (Tianjin Normal University), Ministry of Education, Tianjin Key Laboratory of Structure and Performance for Functional Molecule, College of Chemistry, Tianjin Normal University, 393 Binshui West Road, Tianjin 300387, PR China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Key Laboratory of Inorganic-Organic Hybrid Functional Material Chemistry (Tianjin Normal University), Ministry of Education, Tianjin Key Laboratory of Structure and Performance for Functional Molecule, College of Chemistry, Tianjin Normal University, 393 Binshui West Road, Tianjin 300387, PR China.
authors.forename: Qing-Meng
authors.initials: QM
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Inorganic-Organic Hybrid Functional Material Chemistry (Tianjin Normal University), Ministry of Education, Tianjin Key Laboratory of Structure and Performance for Functional Molecule, College of Chemistry, Tianjin Normal University, 393 Binshui West Road, Tianjin 300387, PR China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Inorganic-Organic Hybrid Functional Material Chemistry (Tianjin Normal University), Ministry of Education, Tianjin Key Laboratory of Structure and Performance for Functional Molecule, College of Chemistry, Tianjin Normal University, 393 Binshui West Road, Tianjin 300387, PR China. Electronic address: nkliyan398@gmail.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Inorganic-Organic Hybrid Functional Material Chemistry (Tianjin Normal University), Ministry of Education, Tianjin Key Laboratory of Structure and Performance for Functional Molecule, College of Chemistry, Tianjin Normal University, 393 Binshui West Road, Tianjin 300387, PR China.
authors.forename: Jian-Zhong
authors.initials: JZ
authors.lastname: Huo
authors.affiliation: Key Laboratory of Inorganic-Organic Hybrid Functional Material Chemistry (Tianjin Normal University), Ministry of Education, Tianjin Key Laboratory of Structure and Performance for Functional Molecule, College of Chemistry, Tianjin Normal University, 393 Binshui West Road, Tianjin 300387, PR China.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Zhao
mesh_terms: D001897:Boronic Acids / Q000138:chemical synthesis*; D003116:Color; D003300:Copper / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D006023:Glycoproteins / Q000032:analysis / Q000097:blood* / Q000737:chemistry; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D001897:Boronic Acids; D005456:Fluorescent Dyes; D006023:Glycoproteins; D012710:Serum Albumin, Bovine; C028592:3-aminobenzeneboronic acid; D003300:Copper
keywords: Boracic acid functionalization; Copper nanoclusters; Facile synthesis; Glycoproteins