title: Hyaluronic Acid Derivative with Improved Versatility for Processing and Biological Functionalization.
abstract: A hydrophobic/amino functionalized derivative of hyaluronic acid (HA-EDA-C18) has been processed by salt leaching technique as porous scaffold without need of chemical crosslinking. Aim of this work is to demonstrate the improved versatility of HA-EDA-C18in terms of processing and biological functionalization. In particular, the chemical procedure to tether thiol bearing RGD peptide has been described. Moreover, the possibility to load and to control the release of slightly water soluble effectors has been demonstrated by using dexamethasone. First, the swelling and degradation profiles of the scaffolds have been investigated, then the evaluation of metabolic activity of bovine chondrocytes, the histological analysis, and microscope observations has been performed to evaluate cellular adhesion and proliferation as well as the production of collagen type II.
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche, Sezione di Chimica e Tecnologie Farmaceutiche, Università degli Studi di Palermo, Via Archirafi 32, 90123, Palermo, Italy. fabiosalvatore.palumbo@unipa.it.
authors.forename: Fabio S
authors.initials: FS
authors.lastname: Palumbo
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche, Sezione di Chimica e Tecnologie Farmaceutiche, Università degli Studi di Palermo, Via Archirafi 32, 90123, Palermo, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Agnello
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche, Sezione di Chimica e Tecnologie Farmaceutiche, Università degli Studi di Palermo, Via Archirafi 32, 90123, Palermo, Italy.
authors.forename: Calogero
authors.initials: C
authors.lastname: Fiorica
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche, Sezione di Chimica e Tecnologie Farmaceutiche, Università degli Studi di Palermo, Via Archirafi 32, 90123, Palermo, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Pitarresi
authors.affiliation: Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri", Histopathology and Immunohistochemistry Laboratory, Palermo, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Puleio
authors.affiliation: Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri", Histopathology and Immunohistochemistry Laboratory, Palermo, Italy.
authors.forename: Anna
authors.initials: A
authors.lastname: Tamburello
authors.affiliation: Istituto Zooprofilattico Sperimentale della Sicilia "A. Mirri", Histopathology and Immunohistochemistry Laboratory, Palermo, Italy.
authors.forename: Ruggero
authors.initials: R
authors.lastname: Loria
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche, Sezione di Chimica e Tecnologie Farmaceutiche, Università degli Studi di Palermo, Via Archirafi 32, 90123, Palermo, Italy.
authors.forename: Gaetano
authors.initials: G
authors.lastname: Giammona
mesh_terms: D000818:Animals; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism*; D024043:Collagen Type II / Q000096:biosynthesis; D003907:Dexamethasone / Q000737:chemistry / Q000494:pharmacology; D006820:Hyaluronic Acid / Q000737:chemistry / Q000494:pharmacology; D016062:Porosity; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D024043:Collagen Type II; D003907:Dexamethasone; D006820:Hyaluronic Acid
keywords: RGD; collagen type II; dexamethasone; drug release; hyaluronic acid derivatives; maleimide chemistry