title: Heat processing of peanut seed enhances the sensitization potential of the major peanut allergen Ara h 6.
abstract: Processing of food has been shown to impact IgE binding and functionality of food allergens. In the present study, we investigated the impact of heat processing on the sensitization capacity of Ara h 6, a major peanut allergen and one of the most potent elicitors of the allergic reaction.
  
  
  
  Peanut extracts obtained from raw or heat-processed peanut and some fractions thereof were biochemically and immunochemically characterized. These extracts/fractions, purified Ara h 6, or recombinant Ara h 6 including Ara h 6 mutants lacking disulfide bridges were used in in vitro digestion tests and mouse models of experimental sensitization. Peanut roasting led to the formation of complexes of high molecular weight, notably between Ara h 6 and Ara h 1, which supported the induction of IgE specific to native Ara h 6. On the contrary, a fraction containing free monomeric 2S albumins or purified native Ara h 6 displayed no intrinsic allergenicity. In addition to complex formation, heat denaturation and/or partial destabilization enhanced Ara h 6 immunogenicity and increased its sensitivity to digestion.
  
  
  
  These results suggest that sensitization potency and IgE binding capacity can be supported by different structures, modified and/or produced during food processing in interaction with other food constituents.
authors.affiliation: UMR CEA-INRA Service de Pharmacologie et d'Immunoanalyse, Université Paris-Saclay, Gif-sur-Yvette, France.
authors.forename: Blanche
authors.initials: B
authors.lastname: Guillon
authors.affiliation: UMR CEA-INRA Service de Pharmacologie et d'Immunoanalyse, Université Paris-Saclay, Gif-sur-Yvette, France.
authors.forename: Hervé
authors.initials: H
authors.lastname: Bernard
authors.affiliation: UMR CEA-INRA Service de Pharmacologie et d'Immunoanalyse, Université Paris-Saclay, Gif-sur-Yvette, France.
authors.forename: Marie-Françoise
authors.initials: MF
authors.lastname: Drumare
authors.affiliation: UMR CEA-INRA Service de Pharmacologie et d'Immunoanalyse, Université Paris-Saclay, Gif-sur-Yvette, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Hazebrouck
authors.affiliation: UMR CEA-INRA Service de Pharmacologie et d'Immunoanalyse, Université Paris-Saclay, Gif-sur-Yvette, France.
authors.forename: Karine
authors.initials: K
authors.lastname: Adel-Patient
mesh_terms: D055516:2S Albumins, Plant / Q000276:immunology*; D000818:Animals; D052179:Antigens, Plant / Q000276:immunology*; D010367:Arachis / Q000737:chemistry / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D005511:Food Handling; D006358:Hot Temperature; D051379:Mice; D008807:Mice, Inbred BALB C; D021183:Peanut Hypersensitivity / Q000276:immunology*; D012639:Seeds / Q000737:chemistry / Q000276:immunology*
chemical_list: D055516:2S Albumins, Plant; D052179:Antigens, Plant; C492737:Ara h 6 allergen, Arachis hypogaea
keywords: Allergenicity assessment; Ara h 6; Food processing; IgE induction; in vitro digestion test