title: Immobilization of the Gas Signaling Molecule H2 S by Radioisotopes: Detection, Quantification, and In Vivo Imaging.
abstract: Hydrogen sulfide (H2 S) has multifunctional roles as a gas signaling molecule in living systems. However, the efficient detection and imaging of H2 S in live animals is very challenging. Herein, we report the first radioisotope-based immobilization technique for the detection, quantification, and in vivo imaging of endogenous H2 S. Macrocyclic (64) Cu complexes that instantly reacted with gaseous H2 S to form insoluble (64) CuS in a highly sensitive and selective manner were prepared. The H2 S concentration in biological samples was measured by a thin-layer radiochromatography method. When (64) Cu-cyclen was injected into mice, an elevated H2 S concentration in the inflamed paw was clearly visualized and quantified by Cerenkov luminescence and positron emission tomography (PET) imaging. PET imaging was also able to pinpoint increased H2 S levels in a millimeter-sized infarcted lesion of the rat heart.
authors.affiliation: Department of Molecular Medicine, Department of Anatomy, BK21 Plus Program, Kyungpook National University, Daegu, 41944, Korea.
authors.forename: Swarbhanu
authors.initials: S
authors.lastname: Sarkar
authors.affiliation: Department of Molecular Medicine, Department of Anatomy, BK21 Plus Program, Kyungpook National University, Daegu, 41944, Korea.
authors.forename: Yeong Su
authors.initials: YS
authors.lastname: Ha
authors.affiliation: Department of Molecular Medicine, Department of Anatomy, BK21 Plus Program, Kyungpook National University, Daegu, 41944, Korea.
authors.forename: Nisarg
authors.initials: N
authors.lastname: Soni
authors.affiliation: Molecular Imaging Research Centre, Korea Institute of Radiological and Medical Sciences, Seoul, 01812, Korea.
authors.forename: Gwang Il
authors.initials: GI
authors.lastname: An
authors.affiliation: Department of Molecular Medicine, Department of Anatomy, BK21 Plus Program, Kyungpook National University, Daegu, 41944, Korea.
authors.forename: Woonghee
authors.initials: W
authors.lastname: Lee
authors.affiliation: Molecular Imaging Research Centre, Korea Institute of Radiological and Medical Sciences, Seoul, 01812, Korea.
authors.forename: Min Hwan
authors.initials: MH
authors.lastname: Kim
authors.affiliation: Department of Molecular Medicine, Department of Anatomy, BK21 Plus Program, Kyungpook National University, Daegu, 41944, Korea.
authors.forename: Phuong Tu
authors.initials: PT
authors.lastname: Huynh
authors.affiliation: Department of Molecular Medicine, Department of Anatomy, BK21 Plus Program, Kyungpook National University, Daegu, 41944, Korea.
authors.forename: Heesu
authors.initials: H
authors.lastname: Ahn
authors.affiliation: Department of Molecular Medicine, Department of Anatomy, BK21 Plus Program, Kyungpook National University, Daegu, 41944, Korea.
authors.forename: Nikunj
authors.initials: N
authors.lastname: Bhatt
authors.affiliation: Molecular Imaging Research Centre, Korea Institute of Radiological and Medical Sciences, Seoul, 01812, Korea.
authors.forename: Yong Jin
authors.initials: YJ
authors.lastname: Lee
authors.affiliation: Molecular Imaging Research Centre, Korea Institute of Radiological and Medical Sciences, Seoul, 01812, Korea.
authors.forename: Jung Young
authors.initials: JY
authors.lastname: Kim
authors.affiliation: Department of Molecular Medicine, Department of Anatomy, BK21 Plus Program, Kyungpook National University, Daegu, 41944, Korea.
authors.forename: Kwon Moo
authors.initials: KM
authors.lastname: Park
authors.affiliation: Department of Biochemistry, Keio University, Tokyo, 105-8512, Japan.
authors.forename: Isao
authors.initials: I
authors.lastname: Ishii
authors.affiliation: Department of Chemistry, Daegu University, Gyeongsan, 38453, Korea.
authors.forename: Shin-Geol
authors.initials: SG
authors.lastname: Kang
authors.affiliation: Department of Molecular Medicine, Department of Anatomy, BK21 Plus Program, Kyungpook National University, Daegu, 41944, Korea. yooj@knu.ac.kr.
authors.forename: Jeongsoo
authors.initials: J
authors.lastname: Yoo
mesh_terms: D000818:Animals; D003301:Copper Radioisotopes / Q000008:administration & dosage / Q000737:chemistry*; D005740:Gases / Q000032:analysis; D006862:Hydrogen Sulfide / Q000032:analysis*; D051379:Mice; D061848:Optical Imaging; D009942:Organometallic Compounds / Q000008:administration & dosage / Q000737:chemistry*; D049268:Positron-Emission Tomography; D051381:Rats
chemical_list: D003301:Copper Radioisotopes; D005740:Gases; D009942:Organometallic Compounds; D006862:Hydrogen Sulfide
keywords: gas immobilization; hydrogen sulfide; imaging agents; nuclear imaging; radioisotopes