title: Performance of Alere™ immunochromathographic test for the diagnosis of canine visceral leishmaniasis.
abstract: The diagnosis and control of canine visceral leishmaniasis (CVL) has fundamental importance in the control of human visceral leishmaniasis. In this context, the immunochromathographic test (ICT) has emerged as a valuable diagnostic tool. Currently, in Brazil, the algorithm for the diagnosis of CVL uses Dual Path Platform (DPP) ICT as a screening test and ELISA as a confirmatory test. However, the DPP(®) ICT has been facing a problem of insufficient production to meet the national demand. Here, the Alere™ canine visceral leishmaniasis ICT was tested with serum samples from 159 dogs, including symptomatic, asymptomatic and healthy dogs, characterized by DPP(®) ICT and ELISA (panel A), or ELISA and Indirect Immunofluorescence Assay (IFA) (panel B), and parasitological examination as gold standard. In this study, the Alere™ ICT obtained 0.974 sensitivity for panel A and 0.854 sensitivity for panel B. The specificity was 1.0 for both panels. Thus, the Alere™ ICT has potential to be used for the serological diagnosis of CVL.
authors.affiliation: Brazilian National Reference Laboratory for Diagnosis of Visceral Leishmaniasis, Serviço de Doenças Parasitárias, Instituto Octavio Magalhães, Fundação Ezequiel Dias (FUNED), Belo Horizonte-MG, Brazil. Electronic address: job.filho@funed.mg.gov.br.
authors.forename: Job Alves de
authors.initials: JA
authors.lastname: Souza Filho
authors.affiliation: Brazilian National Reference Laboratory for Diagnosis of Visceral Leishmaniasis, Serviço de Doenças Parasitárias, Instituto Octavio Magalhães, Fundação Ezequiel Dias (FUNED), Belo Horizonte-MG, Brazil.
authors.forename: José Ronaldo
authors.initials: JR
authors.lastname: Barbosa
authors.affiliation: Instituto Nacional de Infectologia Evandro Chagas/Fundação Oswaldo Cruz (FioCruz), Rio de Janeiro-RJ, Brazil.
authors.forename: Fabiano Borges
authors.initials: FB
authors.lastname: Figueiredo
authors.affiliation: Instituto Nacional de Infectologia Evandro Chagas/Fundação Oswaldo Cruz (FioCruz), Rio de Janeiro-RJ, Brazil.
authors.forename: Artur Augusto Velho
authors.initials: AA
authors.lastname: Mendes
authors.affiliation: Centro de Pesquisas René Rachou, Fiocruz, Belo Horizonte, Minas Gerais, Brazil.
authors.forename: Shara Regina da
authors.initials: SR
authors.lastname: Silva
authors.affiliation: Escola de Medicina, Universidade Federal de Ouro Preto, Ouro Preto-MG, Brazil.
authors.forename: George Luiz Lins Machado
authors.initials: GL
authors.lastname: Coelho
authors.affiliation: Brazilian National Reference Laboratory for Diagnosis of Visceral Leishmaniasis, Serviço de Doenças Parasitárias, Instituto Octavio Magalhães, Fundação Ezequiel Dias (FUNED), Belo Horizonte-MG, Brazil. Electronic address: andreza.marcelino@funed.mg.gov.br.
authors.forename: Andreza Pain
authors.initials: AP
authors.lastname: Marcelino
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D001938:Brazil; D019937:Diagnostic Techniques and Procedures / Q000592:standards / Q000662:veterinary*; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D019084:Fluorescent Antibody Technique, Indirect / Q000592:standards / Q000662:veterinary; D018314:Leishmania infantum; D007898:Leishmaniasis, Visceral / Q000175:diagnosis / Q000662:veterinary*; D012680:Sensitivity and Specificity
chemical_list: D000913:Antibodies, Protozoan
keywords: Alere; Canine visceral leishmaniasis; Immunochromathographic test