title: Inactivation of viruses during a new manufacturing process of α2-macroglobulin from Cohn Fraction IV by dry-heat treatment.
abstract: α2-Macroglobulin (α2-M) has a curative effect on radiation injury. Virus transmission through plasma derivatives is still not risk-free. Effect of dry heat on α2-M activity and virus inactivation by dry heat in a new manufacturing process of α2-M were studied.
  
  
  
  Effects of 100°C for 30 minutes, 80°C for 72 hours, and lyophilization on α2-M activity were detected, and stabilizing agents were optimized. Effect of a treatment at 100°C for 30 minutes has been tested on a range of viruses and characteristics change of α2-M was investigated.
  
  
  
  More than 90 and 80% α2-M activity recovery were reserved after treatment at 100°C for 30 minutes and 80°C for 72 hours, respectively. A concentration of 0.05 mol/L histidine presented a better protecting effect for α-M activity. No substantial changes were observed in the characteristics of α2-M compared with the untreated. By lyophilization and dry-heat treatment at 100°C for 30 minutes, murine encephalomyocarditis virus and pseudorabies virus (PRV) were inactivated below detectable level within 5 minutes (virus titers reduction ≥ 5.75 log) and 30 minutes (virus titers reduction ≥ 6.00 log), respectively. Bovine viral diarrhea virus and porcine parvovirus were inactivated by 4.29 and 2.46 log reduction, respectively.
  
  
  
  Treatment at 100°C for 30 minutes could improve the virus safety of α2-M with a slight activity loss.
authors.affiliation: Beijing Key Laboratory of Blood Safety and Supply Technologies & Blood Products and Substitute Laboratory, Beijing Institute of Transfusion Medicine, Beijing, China.
authors.forename: Chaoji
authors.initials: C
authors.lastname: Huangfu
authors.affiliation: Beijing Key Laboratory of Blood Safety and Supply Technologies & Blood Products and Substitute Laboratory, Beijing Institute of Transfusion Medicine, Beijing, China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Beijing Key Laboratory of Blood Safety and Supply Technologies & Blood Products and Substitute Laboratory, Beijing Institute of Transfusion Medicine, Beijing, China.
authors.forename: Maomin
authors.initials: M
authors.lastname: Lv
authors.affiliation: Beijing Key Laboratory of Blood Safety and Supply Technologies & Blood Products and Substitute Laboratory, Beijing Institute of Transfusion Medicine, Beijing, China.
authors.forename: Junting
authors.identifier: 0000-0001-5883-6925
authors.initials: J
authors.lastname: Jia
authors.affiliation: Beijing Key Laboratory of Blood Safety and Supply Technologies & Blood Products and Substitute Laboratory, Beijing Institute of Transfusion Medicine, Beijing, China.
authors.forename: Fengxuan
authors.initials: F
authors.lastname: Zhu
authors.affiliation: Beijing Key Laboratory of Blood Safety and Supply Technologies & Blood Products and Substitute Laboratory, Beijing Institute of Transfusion Medicine, Beijing, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.affiliation: Beijing Key Laboratory of Blood Safety and Supply Technologies & Blood Products and Substitute Laboratory, Beijing Institute of Transfusion Medicine, Beijing, China. mayuyuan07@hotmail.com.
authors.forename: Yuyuan
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Beijing Key Laboratory of Blood Safety and Supply Technologies & Blood Products and Substitute Laboratory, Beijing Institute of Transfusion Medicine, Beijing, China. zhangjg@bmi.ac.cn.
authors.forename: Jingang
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001793:Blood Preservation / Q000379:methods*; D001798:Blood Proteins / Q000737:chemistry*; D002460:Cell Line; D004285:Dogs; D006358:Hot Temperature; D006801:Humans; D038301:Virus Inactivation; D000511:alpha-Macroglobulins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D001798:Blood Proteins; C032309:Cohn fraction IV; D000511:alpha-Macroglobulins