title: Interactions "Candidatus Liberibacter solanacearum"-Bactericera cockerelli: Haplotype Effect on Vector Fitness and Gene Expression Analyses.
abstract: "Candidatus Liberibacter solanacearum" (Lso) has emerged as a serious threat world-wide. Five Lso haplotypes have been identified so far. Haplotypes A and B are present in the Americas and/or New Zealand, where they are vectored to solanaceous plants by the potato psyllid, Bactericera cockerelli (Šulc) (Hemiptera: Triozidae). The fastidious nature of these pathogens has hindered the study of the interactions with their eukaryotic hosts (vector and plant). To understand the strategies used by these pathogens to infect their vector, the effects of each Lso haplotype (A or B) on psyllid fitness was investigated, and genome-wide transcriptomic and RT-qPCR analyses were performed to evaluate Lso gene expression in association with its vector. Results showed that psyllids infected with haplotype B had significantly lower percentage of nymphal survival compared to psyllids infected with haplotype A. Although overall gene expression across Lso genome was similar between the two Lso haplotypes, differences in the expression of key candidate genes were found. Among the 16 putative type IV effector genes tested, four of them were differentially expressed between Lso haplotypes, while no differences in gene expression were measured by qPCR or transcriptomic analysis for the rest of the genes. This study provides new information regarding the pathogenesis of Lso haplotypes in their insect vector.
authors.affiliation: Department of Entomology, Texas A&M University College Station, TX, USA.
authors.forename: Jianxiu
authors.initials: J
authors.lastname: Yao
authors.affiliation: Department of Entomology, Texas A&M University College Station, TX, USA.
authors.forename: Panatda
authors.initials: P
authors.lastname: Saenkham
authors.affiliation: Department of Horticultural Sciences, Texas A&M University College Station, TX, USA.
authors.forename: Julien
authors.initials: J
authors.lastname: Levy
authors.affiliation: Department of Entomology, Texas A&M University College Station, TX, USA.
authors.forename: Freddy
authors.initials: F
authors.lastname: Ibanez
authors.affiliation: CIRAD, UMR CMAEEPetit-Bourg, Guadeloupe, France; Institut National de la Recherche Agronomique, UMR1309 CMAEEMontpellier, France; Université des Antilles, Pointe-à-PitreGuadeloupe, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Noroy
authors.affiliation: Department of Entomology, Texas A&M University College Station, TX, USA.
authors.forename: Azucena
authors.initials: A
authors.lastname: Mendoza
authors.affiliation: Department of Entomology, Texas A&M University College Station, TX, USA.
authors.forename: Ordom
authors.initials: O
authors.lastname: Huot
authors.affiliation: CIRAD, UMR CMAEEPetit-Bourg, Guadeloupe, France; Institut National de la Recherche Agronomique, UMR1309 CMAEEMontpellier, France.
authors.forename: Damien F
authors.initials: DF
authors.lastname: Meyer
authors.affiliation: Department of Entomology, Texas A&M University College Station, TX, USA.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Tamborindeguy
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D006239:Haplotypes; D006430:Hemiptera / Q000382:microbiology / Q000502:physiology*; D054884:Host-Pathogen Interactions; D007303:Insect Vectors / Q000382:microbiology / Q000502:physiology*; D060888:Real-Time Polymerase Chain Reaction; D012230:Rhizobiaceae / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity*; D016019:Survival Analysis
keywords: Bactericera cockerelli; Candidatus Liberibacter solanacearum; Lso haplotype; effectors; psyllid