title: Triggering Death of Adherent Cells with Ultraviolet Radiation.
abstract: Ultraviolet (UV) radiation is a convenient stimulus for triggering cell death that is available in most laboratories. We use a Stratalinker UV cross-linker because it is a safe, cheap, reliable, consistent, and easily controlled source of UV irradiation. This protocol describes using a Stratalinker to trigger UV-induced death of HeLa cells.
authors.affiliation: Apoptosis and Cytotoxicity Laboratory, Mater Research, Translational Research Institute, Woolloongabba, Brisbane, Queensland 4102, Australia;
authors.forename: Lisa C
authors.initials: LC
authors.lastname: Crowley
authors.affiliation: Apoptosis and Cytotoxicity Laboratory, Mater Research, Translational Research Institute, Woolloongabba, Brisbane, Queensland 4102, Australia; Flow Cytometry and Imaging, QIMR Berghofer Medical Research Institute, Herston, Brisbane, Queensland 4006, Australia; School of Medicine, University of Queensland, St. Lucia, Brisbane, Queensland 4072, Australia.
authors.forename: Nigel J
authors.initials: NJ
authors.lastname: Waterhouse
mesh_terms: D016923:Cell Death; D006367:HeLa Cells / Q000528:radiation effects*; D006801:Humans; D014466:Ultraviolet Rays