title: Shotgun Lipidomic Profiling of the NCI60 Cell Line Panel Using Rapid Evaporative Ionization Mass Spectrometry.
abstract: Rapid evaporative ionization mass spectrometry (REIMS) was used for the rapid mass spectrometric profiling of cancer cell lines. Spectral reproducibility was assessed for three different cell lines, and the extent of interclass differences and intraclass variance was found to allow the identification of these cell lines based on the REIMS data. Subsequently, the NCI60 cell line panel was subjected to REIMS analysis, and the resulting data set was investigated for its distinction of individual cell lines and different tissue types of origin. Information content of REIMS spectral profiles of cell lines were found to be similar to those obtained from mammalian tissues although pronounced differences in relative lipid intensity were observed. Ultimately, REIMS was shown to detect changes in lipid content of cell lines due to mycoplasma infection. The data show that REIMS is an attractive means to study cell lines involving minimal sample preparation and analysis times in the range of seconds.
authors.affiliation: Department of Surgery and Cancer, Imperial College London , London SW7 2AZ, U.K.
authors.forename: Nicole
authors.initials: N
authors.lastname: Strittmatter
authors.affiliation: Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences , 1113 Budapest, Hungary.
authors.forename: Anna
authors.initials: A
authors.lastname: Lovrics
authors.affiliation: Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences , 1113 Budapest, Hungary.
authors.forename: Judit
authors.initials: J
authors.lastname: Sessler
authors.affiliation: Department of Surgery and Cancer, Imperial College London , London SW7 2AZ, U.K.
authors.forename: James S
authors.initials: JS
authors.lastname: McKenzie
authors.affiliation: Department of Surgery and Cancer, Imperial College London , London SW7 2AZ, U.K.
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Bodai
authors.affiliation: Department of Surgery and Cancer, Imperial College London , London SW7 2AZ, U.K.
authors.forename: M Luisa
authors.initials: ML
authors.lastname: Doria
authors.affiliation: Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences , 1113 Budapest, Hungary.
authors.forename: Nora
authors.initials: N
authors.lastname: Kucsma
authors.affiliation: Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences , 1113 Budapest, Hungary.|Institute of Cancer Research, Department of Medicine I, Comprehensive Cancer Center, Medical University of Vienna , 1090 Vienna, Austria.
authors.forename: Gergely
authors.initials: G
authors.lastname: Szakacs
authors.affiliation: Department of Surgery and Cancer, Imperial College London , London SW7 2AZ, U.K.
authors.forename: Zoltan
authors.initials: Z
authors.lastname: Takats
mesh_terms: D045744:Cell Line, Tumor / Q000382:microbiology; D006801:Humans; D008055:Lipids / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods; D009174:Mycoplasma; D009369:Neoplasms / Q000378:metabolism; D025341:Principal Component Analysis; D015203:Reproducibility of Results
chemical_list: D008055:Lipids