title: Self-Assembled Heterojunction Carbon Nanotubes Synergizing with Photoimmobilized IGF-1 Inhibit Cellular Senescence.
abstract: Synthesis of artificial and functional structures for bone tissue engineering has been well recognized but the associated cell senescence issue remains much less concerned so far. In this work, surface-modified polycaprolactone-polylactic acid scaffolds using self-assembled heterojunction carbon nanotubes (sh-CNTs) combined with insulin-like growth factor-1 are synthesized and a series of structural and biological characterizations are carried out, with particular attention to cell senescence mechanism. It is revealed that the modified scaffolds can up-regulate the expressions of alkaline phosphates and bone morphogenetic proteins while down-regulate the expressions of senescence-related proteins in mesenchymal stem cells, demonstrating the highly preferred anti-senescence functionality of the sh-CNTs modified scaffolds in bone tissue engineering. Furthermore, it is also found that with sh-CNTs, scaffolds can accelerate bone healing with extremely low toxicity in vivo.
authors.affiliation: School of Life Science, South China Normal University, Guangzhou, 510631, P. R. China.
authors.forename: Wu-Ya
authors.initials: WY
authors.lastname: Chen
authors.affiliation: School of Life Science, South China Normal University, Guangzhou, 510631, P. R. China.
authors.forename: Run-Cai
authors.initials: RC
authors.lastname: Yang
authors.affiliation: School of Life Science, South China Normal University, Guangzhou, 510631, P. R. China.
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Wang
authors.affiliation: School of Life Science, South China Normal University, Guangzhou, 510631, P. R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Biophotonics, South China Normal University, Guangzhou, 510631, P. R. China.
authors.forename: Kaikai
authors.initials: K
authors.lastname: Hu
authors.affiliation: School of Life Science, South China Normal University, Guangzhou, 510631, P. R. China.
authors.forename: Chu-Hua
authors.initials: CH
authors.lastname: Li
authors.affiliation: School of Life Science, South China Normal University, Guangzhou, 510631, P. R. China.
authors.forename: Rong
authors.initials: R
authors.lastname: You
authors.affiliation: School of Life Science, South China Normal University, Guangzhou, 510631, P. R. China.
authors.forename: Liang
authors.initials: L
authors.lastname: Yin
authors.affiliation: School of Life Science, South China Normal University, Guangzhou, 510631, P. R. China. guanyq@scnu.edu.cn.|College of Biophotonics, South China Normal University, Guangzhou, 510631, P. R. China. guanyq@scnu.edu.cn.
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Guan
mesh_terms: D000818:Animals; D002460:Cell Line; D016922:Cellular Senescence; D055767:Immobilized Proteins / Q000737:chemistry; D007334:Insulin-Like Growth Factor I / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D051381:Rats
chemical_list: D055767:Immobilized Proteins; D037742:Nanotubes, Carbon; D011091:Polyesters; C548904:insulin-like growth factor-1, rat; C016240:polycaprolactone; C033616:poly(lactide); D007334:Insulin-Like Growth Factor I
keywords: IGF-1; bone tissue engineering; cellular senescence; sh-CNT