title: Chemical composition, antioxidant and antimicrobial effects of Tunisian Limoniastrum guyonianum Durieu ex Boiss extracts.
abstract: In the present investigation, extracts obtained from L. guyonianum Durieu ex Boiss. aerial parts were used to evaluate total phenolic, flavonoid and tannin contents. A study of antioxidant activities of the prepared samples was carried out on the basis of 1,1-Diphenyl-2-picrylhydrazyl (DPPH), 2-2'-azino-bis-(3-ethylbenz-thiazoline-6-sulfonic acid) (ABTS+.) and ferric reducing antioxidant power (FRAP) assays. Moreover, the efficiency of methanolic, chloroformic and petroleum ether extracts and the deriving fractions from the methanolic extract was tested against human bacterial and fungal pathogenic strains using micro dilution method in 96 multiwell microtiter plate. Furthermore, leaves and stems extracts were subjected to RP-HPLC for phenolic compounds identification. Results showed that polyphenolic contents and antioxidant activities varied considerably as function of solvent polarity. Moreover, antiradical capacities against DPPH, ABTS(+.) and reducing power were maxima in methanol aerial parts extract which showed the highest polyphenol contents (134mg CE/g DW). The antimicrobial activities showed that methanolic, chloroformic and petroleum ether extracts were found to be most potent against Pseudomonas aeruginosa and Staphylococcus aureus with MIC values of 23 and 46μ.mL(-1), respectively. The fractions F(13) and F(16) have a great antifungal potential against Candida glabrata, Candida krusei and Candida parapsilesis (MIC=39μ.mL(-1)). The RP-HPLC analysis lead the identification of gallic, procatechuic and trans-cinnamic acids, methyl-4-hydroxybenzoate, n-propyl-3,4,5-trihydroxybenzoate, epicatechin, naringin and myricetin in L. guyonianum Durieu ex Boiss. leaves and stems extracts.
authors.affiliation: Laboratory of Bioressources, Biology Integrative and Valorization, Higher Institute of Biotechnology of Monastir, University of Monastir, Avenue Tahar Hadded, BP 74, Monastir, Tunisia.
authors.forename: Amel
authors.initials: A
authors.lastname: Bouzidi
authors.affiliation: Research Applied Chemistry and Environment, Faculty of Sciences of Monastir, Monastir, University of Monastir, Tunisia.
authors.forename: Anissa
authors.initials: A
authors.lastname: Benzarti
authors.affiliation: Laboratory of Bioressources, Biology Integrative and Valorization, Higher Institute of Biotechnology of Monastir, University of Monastir, Avenue Tahar Hadded, BP 74, Monastir, Tunisia.
authors.forename: Amira El
authors.initials: AE
authors.lastname: Arem
authors.affiliation: Laboratory of Bioressources, Biology Integrative and Valorization, Higher Institute of Biotechnology of Monastir, University of Monastir, Avenue Tahar Hadded, BP 74, Monastir, Tunisia.
authors.forename: Adel
authors.initials: A
authors.lastname: Mahfoudhi
authors.affiliation: Research Applied Chemistry and Environment, Faculty of Sciences of Monastir, Monastir, University of Monastir, Tunisia.
authors.forename: Saoussen
authors.initials: S
authors.lastname: Hammami
authors.affiliation: Laboratory of Parasitology-Mycology CHU F. Bourguiba, Monastir, Tunisia.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Gorcii
authors.affiliation: Laboratory of Microbiology CHU F. Bourguiba, Monastir, Tunisia.
authors.forename: Maha
authors.initials: M
authors.lastname: Mastouri
authors.affiliation: Laboratory of Bioressources, Biology Integrative and Valorization, Higher Institute of Biotechnology of Monastir, University of Monastir, Avenue Tahar Hadded, BP 74, Monastir, Tunisia.
authors.forename: Ahmed Noureddine
authors.initials: AN
authors.lastname: Hellal
authors.affiliation: Laboratory of Bioressources, Biology Integrative and Valorization, Higher Institute of Biotechnology of Monastir, University of Monastir, Avenue Tahar Hadded, BP 74, 5000 Monastir, Tunisia / Research Unit 12-04, Applied Chemistry and Environment, Faculty of Sciences of Monastir, 5000 Monastir, University of Monastir, Tunisia.
authors.forename: Zine
authors.initials: Z
authors.lastname: Mighri
mesh_terms: D000890:Anti-Infective Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves; D029606:Plumbaginaceae / Q000737:chemistry*; D059808:Polyphenols / Q000032:analysis; D055051:Salt-Tolerant Plants / Q000737:chemistry*
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D005419:Flavonoids; D010936:Plant Extracts; D059808:Polyphenols