title: Paenibacillus segetis sp. nov., isolated from soil of a tropical rainforest.
abstract: A Gram-stain-positive, facultatively anaerobic, endospore-forming, irregular rod-shaped bacterium, designated DB13260T, was isolated from tropical rainforest soil in Jianfengling Nature Reserve in Hainan, China. The isolate was found to grow with 0-4 % (w/v) NaCl, at 5-40 °C and pH 6.0-10.5, with an optimum of 0 % NaCl, 30-37 °C and pH 8.5-9.0, respectively. The predominant isoprenoid quinone was menaquinone 7 (MK-7), and the major fatty acids were anteiso-C15 : 0, iso-C16 : 0 and C16 : 0. The G+C content of the genomic DNA was 53.7 mol%. Analysis of the 16S rRNA gene sequence of strain DB13260T showed an affiliation of the strain with the genus Paenibacillus, sharing 98.3 % and 97.8 % 16S rRNA gene sequence similarities with the closest relatives Paenibacillus anaericanus MH21T and Paenibacillus selenii W126T, respectively. The DNA-DNA hybridization values between strain DB13260T and the two type strains were 60.4 % and 42.6 %, respectively. The combined phenotypic and DNA-DNA hybridization data supported the conclusion that strain DB13260T represents a novel species of the genus Paenibacillus, for which the name Paenibacillussegetis sp. nov. is proposed. The type strain is DB13260T (=CGMCC 1.12769T=DSM 28014T).
authors.affiliation: Institute of Tropical Biosciences and Biotechnology, Key Laboratory of Biology and Genetic Resources of Tropical Crops of Ministry of Agriculture, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, PR China.
authors.forename: Huiqin
authors.initials: H
authors.lastname: Huang
authors.affiliation: Institute of Tropical Biosciences and Biotechnology, Key Laboratory of Biology and Genetic Resources of Tropical Crops of Ministry of Agriculture, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, PR China.
authors.forename: Fengzhao
authors.initials: F
authors.lastname: Feng
authors.affiliation: Institute of Tropical Biosciences and Biotechnology, Key Laboratory of Biology and Genetic Resources of Tropical Crops of Ministry of Agriculture, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: Institute of Tropical Biosciences and Biotechnology, Key Laboratory of Biology and Genetic Resources of Tropical Crops of Ministry of Agriculture, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, PR China.
authors.forename: Fute
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Institute of Tropical Biosciences and Biotechnology, Key Laboratory of Biology and Genetic Resources of Tropical Crops of Ministry of Agriculture, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, PR China.
authors.forename: Qianguang
authors.initials: Q
authors.lastname: Sun
authors.affiliation: Institute of Tropical Biosciences and Biotechnology, Key Laboratory of Biology and Genetic Resources of Tropical Crops of Ministry of Agriculture, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, PR China.
authors.forename: Songyu
authors.initials: S
authors.lastname: Qin
authors.affiliation: Institute of Tropical Biosciences and Biotechnology, Key Laboratory of Biology and Genetic Resources of Tropical Crops of Ministry of Agriculture, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, Hainan, PR China.
authors.forename: Shixiang
authors.initials: S
authors.lastname: Bao
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D056507:Paenibacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D065947:Rainforest; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014329:Tropical Climate; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid; C062629:menaquinone 7