title: Purification of infectious and non-infectious chlamydial particles using iodixanol for density gradient preparation.
abstract: Chlamydiae are obligate intracellular bacteria with two distinct morphological stages, the infectious elementary bodies (EBs) and non-infectious reticulate bodies (RBs). Here we describe a rapid and straightforward protocol for the purification of EBs and RBs involving special density gradients. It has been successfully applied to three chlamydial species.
authors.affiliation: Department of Cell and Molecular Biology, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute (HKI), Jena, Germany. Electronic address: thomas.beder@leibniz-hki.de.
authors.forename: Thomas
authors.initials: T
authors.lastname: Beder
authors.affiliation: Department of Cell and Molecular Biology, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute (HKI), Jena, Germany. Electronic address: Mareike.Scheven@leibniz-hki.de.
authors.forename: Mareike Thea
authors.initials: MT
authors.lastname: Scheven
authors.affiliation: Department of Cell and Molecular Biology, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute (HKI), Jena, Germany. Electronic address: dominique.praetzsch@googlemail.com.
authors.forename: Dominique
authors.initials: D
authors.lastname: Praetzsch
authors.affiliation: Jena University Hospital, Electron Microscopy Center, Jena, Germany; Friedrich Schiller University Jena, Jena, Germany. Electronic address: martin.westermann@uni-jena.de.
authors.forename: Martin
authors.initials: M
authors.lastname: Westermann
authors.affiliation: Department of Cell and Molecular Biology, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute (HKI), Jena, Germany; Friedrich Schiller University Jena, Jena, Germany. Electronic address: hanspeter.saluz@leibniz-hki.de.
authors.forename: Hans Peter
authors.initials: HP
authors.lastname: Saluz
mesh_terms: D002460:Cell Line; D002689:Chlamydia / Q000145:classification / Q000302:isolation & purification*; D015169:Colony Count, Microbial; D003287:Contrast Media / Q000737:chemistry*; D006801:Humans; D012329:RNA, Bacterial / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000302:isolation & purification; D014283:Triiodobenzoic Acids / Q000737:chemistry*
chemical_list: D003287:Contrast Media; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D014283:Triiodobenzoic Acids; C044834:iodixanol
keywords: Intracellular pathogens; Iodixanol; Optiprep™; Preparative ultracentrifugation; Visipaque®