title: [Secretory acid phosphatase of Mycobacterium tuberculosis inhibits the autophagy of murine macrophages].
abstract: Objective To investigate the effect of secretory acid phosphatase as a virulence factor of Mycobacterium tuberculosis (SapM) on the autophagy of murine macrophages. Methods GFP-LC3-Raw264.7 cells were treated with SapM, wortmannin, or starvation. Then the formation of autophagosomes was observed under a fluorescence microscope. The level of microtubule-associated protein 1 light chain 3 (LC3) II was detected using Western blotting. After chloroquine was added in the SapM-treated cells, LC3II level was again tested by Western blotting. Results Both starvation and SapM increased the number of GFP-LC3 puncta and the level of LC3 II. There was no further increase of LC3 II level in SapM-treated cells after chloroquine addition. Conclusion SapM can block autophagosome-lysosome fusion and inhibit autophagy of murine macrophages.
authors.affiliation: Medical Inspection Center, Department of Medical Immunology, Medical School, Anhui University of Science and Technology, Huainan 232001, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Hu
authors.affiliation: Medical Inspection Center, Anhui University of Science and Technology, Huainan 232001, China.
authors.forename: Wan
authors.initials: W
authors.lastname: Wang
authors.affiliation: Medical Inspection Center, Anhui University of Science and Technology, Huainan 232001, China.
authors.forename: Runpeng
authors.initials: R
authors.lastname: Zhao
authors.affiliation: Medical Inspection Center, Anhui University of Science and Technology, Huainan 232001, China.
authors.forename: Xuewei
authors.initials: X
authors.lastname: Xu
authors.affiliation: Affiliated Cancer Hospital, Anhui University of Science and Technology, Huainan 232001, China.
authors.forename: Yingru
authors.initials: Y
authors.lastname: Xing
authors.affiliation: Affiliated Cancer Hospital, Anhui University of Science and Technology, Huainan 232001, China.
authors.forename: Congjing
authors.initials: C
authors.lastname: Xu
authors.affiliation: Medical Inspection Center, Department of Medical Immunology, Medical School, Anhui University of Science and Technology, Huainan 232001, China. *Corresponding authors, E-mail: lory456@126.com.
authors.forename: Rongbo
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Medical Inspection Center, Department of Medical Immunology, Medical School, Anhui University of Science and Technology, Huainan 232001, China. *Corresponding authors, E-mail: wujing8008@126.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
mesh_terms: D000135:Acid Phosphatase / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D000962:Antimalarials / Q000494:pharmacology; D000071182:Autophagosomes / Q000378:metabolism; D001343:Autophagy / Q000235:genetics / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D002460:Cell Line; D002738:Chloroquine / Q000494:pharmacology; D003470:Culture Media / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008247:Lysosomes / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D008561:Membrane Fusion / Q000187:drug effects; D051379:Mice; D008856:Microscopy, Fluorescence; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism; D009169:Mycobacterium tuberculosis / Q000201:enzymology
chemical_list: D000962:Antimalarials; D001426:Bacterial Proteins; D003470:Culture Media; C480860:Map1lc3b protein, mouse; D008869:Microtubule-Associated Proteins; D049452:Green Fluorescent Proteins; D002738:Chloroquine; D000135:Acid Phosphatase