title: A sensitive sandwich-type electrochemical aptasensor for thrombin detection based on platinum nanoparticles decorated carbon nanocages as signal labels.
abstract: In this work, a novel and sensitive sandwich-type electrochemical aptasensor has been developed for thrombin detection based on platinum nanoparticles (Pt NPs) decorated carbon nanocages (CNCs) as signal tags. The morphological and compositional of the Pt NPs/CNCs were examined using transmission electron microscopy, X-ray diffraction, and Raman spectroscopy. The results showed that the Pt NPs with about 3-5nm in diameter were well dispersed on the surface of CNCs. The thiolated aptamer was firstly immobilized on the gold electrode to capture the thrombin molecules, and then aptamer functionalized Pt NPs/CNCs nanocomposites were used to fabricate a sandwich sensing platform. Then, the high-content Pt NPs on carbon nanocages acting as hydrogen peroxide-mimicking enzyme catalyzed the reduction of H2O2, resulting in significant electrochemical signal amplification. Differential pulse voltammetry is employed to detect thrombin with different concentrations. Under optimized conditions, the approach provided a good linear response range from 0.05 pM to 20nM with a low detection limit of 10fM. This Pt NPs/CNCs-based aptasensor shows good precision, acceptable stability and reproducibility, which provided a promising strategy for electrochemical aptamer-based detection of other biomolecules.
authors.affiliation: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy, Xuzhou Medical University, 221004 Xuzhou, China. Electronic address: jsxzgfl@sina.com.
authors.forename: Fenglei
authors.initials: F
authors.lastname: Gao
authors.affiliation: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy, Xuzhou Medical University, 221004 Xuzhou, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Du
authors.affiliation: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy, Xuzhou Medical University, 221004 Xuzhou, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy, Xuzhou Medical University, 221004 Xuzhou, China.
authors.forename: Fuyi
authors.initials: F
authors.lastname: Zhou
authors.affiliation: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy, Xuzhou Medical University, 221004 Xuzhou, China. Electronic address: tdq993@hotmail.com.
authors.forename: Daoquan
authors.initials: D
authors.lastname: Tang
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000235:genetics; D001774:Blood Chemical Analysis / Q000295:instrumentation*; D002244:Carbon / Q000737:chemistry*; D003217:Conductometry / Q000295:instrumentation*; D004553:Electric Conductivity; D004867:Equipment Design; D019544:Equipment Failure Analysis; D053768:Metal Nanoparticles / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry*; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013917:Thrombin / Q000032:analysis* / Q000235:genetics
chemical_list: D052157:Aptamers, Nucleotide; D010984:Platinum; D002244:Carbon; D013917:Thrombin
keywords: Aptasensors; Carbon nanocages; Electrochemical; Thrombin