title: The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development.
abstract: Nucleotide sugar transport across Golgi membranes is essential for the luminal biosynthesis of glycan structures. Here we identify GDP-fucose transporter 1 (GFT1), an Arabidopsis nucleotide sugar transporter that translocates GDP-L-fucose into the Golgi lumen. Using proteo-liposome-based transport assays, we show that GFT preferentially transports GDP-L-fucose over other nucleotide sugars in vitro, while GFT1-silenced plants are almost devoid of L-fucose in cell wall-derived xyloglucan and rhamnogalacturonan II. Furthermore, these lines display reduced L-fucose content in N-glycan structures accompanied by severe developmental growth defects. We conclude that GFT1 is the major nucleotide sugar transporter for import of GDP-L-fucose into the Golgi and is required for proper plant growth and development.
authors.affiliation: Joint BioEnergy Institute, Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94702, USA.|ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Melbourne, VIC 3010, Australia.
authors.forename: Carsten
authors.initials: C
authors.lastname: Rautengarten
authors.affiliation: Joint BioEnergy Institute, Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94702, USA.|ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Melbourne, VIC 3010, Australia.|Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Copenhagen DK-1871, Denmark.
authors.forename: Berit
authors.initials: B
authors.lastname: Ebert
authors.affiliation: Department of Plant and Microbial Biology, University of California, Berkeley, California 94720, USA.
authors.forename: Lifeng
authors.initials: L
authors.lastname: Liu
authors.affiliation: Joint BioEnergy Institute, Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94702, USA.
authors.forename: Solomon
authors.initials: S
authors.lastname: Stonebloom
authors.affiliation: Joint BioEnergy Institute, Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94702, USA.
authors.forename: Andreia M
authors.initials: AM
authors.lastname: Smith-Moritz
authors.affiliation: Department of Plant and Microbial Biology, University of California, Berkeley, California 94720, USA.
authors.forename: Markus
authors.initials: M
authors.lastname: Pauly
authors.affiliation: Centro de Biotecnología Vegetal, Facultad de Ciencias Biológicas, Universidad Andrés Bello, Santiago RM 8370146, Chile.|Fondo de Areas Prioritarias Center for Genome Regulation, Santiago RM 8370146, Chile.
authors.forename: Ariel
authors.initials: A
authors.lastname: Orellana
authors.affiliation: Joint BioEnergy Institute, Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94702, USA.|Department of Plant and Microbial Biology, University of California, Berkeley, California 94720, USA.
authors.forename: Henrik Vibe
authors.initials: HV
authors.lastname: Scheller
authors.affiliation: Joint BioEnergy Institute, Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94702, USA.|ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Melbourne, VIC 3010, Australia.
authors.forename: Joshua L
authors.initials: JL
authors.lastname: Heazlewood
mesh_terms: D017360:Arabidopsis / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D001692:Biological Transport; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism; D003001:Cloning, Molecular; D015870:Gene Expression; D005822:Genetic Vectors / Q000737:chemistry / Q000378:metabolism; D005936:Glucans / Q000096:biosynthesis; D006056:Golgi Apparatus / Q000737:chemistry / Q000378:metabolism*; D006154:Guanosine Diphosphate Fucose / Q000378:metabolism*; D009004:Monosaccharide Transport Proteins / Q000235:genetics* / Q000378:metabolism; D010368:Pectins / Q000096:biosynthesis; D010802:Phylogeny; D059828:Plant Cells / Q000737:chemistry / Q000378:metabolism; D011510:Proteolipids / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014990:Xylans / Q000096:biosynthesis
chemical_list: D029681:Arabidopsis Proteins; D005936:Glucans; D009004:Monosaccharide Transport Proteins; D011510:Proteolipids; D011994:Recombinant Proteins; D014990:Xylans; C020485:proteoliposomes; C042492:rhamnogalacturonan II; D006154:Guanosine Diphosphate Fucose; C029353:xyloglucan; D010368:Pectins