title: The role of apelin in central cardiovascular regulation in rats with post-infarct heart failure maintained on a normal fat or high fat diet.
abstract: Based on the available literature, it can be assumed that in cases of post-infarct heart failure (HF) and obesity, a significant change in the central regulation of the cardiovascular system takes place with, among others, the involvement of the apelinergic system. The main objective of the present study was to clarify the role of apelin-13 in the central regulation of the cardiovascular system in Sprague Dawley rats with HF or sham operated (SO) and fed on a normal fat (NFD) or a high fat diet (HFD). The study was divided into two parts: Part I, hemodynamic studies; and Part II, biochemical and molecular studies. The animals were subjected to the following research procedures. Part I and II: feeding NFD or HFD; experimental induction of HF or SO; Part I: intracerebroventricular (ICV) infusion of the examined substances, monitoring of mean arterial blood pressure (MABP) and heart rate (HR); Part II: venous blood and tissue samples collected. ICV infusion of apelin-13 caused significantly higher changes in ΔMABP in the SO NFD group. No changes were noted in ΔHR in any of the studied groups. Apelin and apelin receptor (APJ) mRNA expression in the brain and adipose tissues was higher in the HF rats. HFD causes significant increase in expression of apelin and APJ mRNA in the left ventricle. In conclusion, HF and HFD appear to play an important role in modifying the activity of the central apelinergic system and significant changes in mRNA expression of apelin and APJ receptor.
authors.affiliation: Department of Experimental and Clinical Physiology, Laboratory of Centre for Preclinical Research, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Czarzasta
authors.affiliation: Department of Experimental and Clinical Physiology, Laboratory of Centre for Preclinical Research, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Cudnoch-Jedrzejewska
authors.affiliation: Department of Experimental and Clinical Physiology, Laboratory of Centre for Preclinical Research, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Szczepanska-Sadowska
authors.affiliation: Department of Pathology, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Lukasz
authors.initials: L
authors.lastname: Fus
authors.affiliation: Department of Experimental and Clinical Physiology, Laboratory of Centre for Preclinical Research, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Liana
authors.initials: L
authors.lastname: Puchalska
authors.affiliation: Department of Experimental and Clinical Physiology, Laboratory of Centre for Preclinical Research, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Agata
authors.initials: A
authors.lastname: Gondek
authors.affiliation: Department of Urology, Centre of Postgraduate Medical Education, Warsaw, Poland.
authors.forename: Jakub
authors.initials: J
authors.lastname: Dobruch
authors.affiliation: Faculty of Electronics and Information Technology, Warsaw University of Technology, Warsaw, Poland.
authors.forename: Ryszard
authors.initials: R
authors.lastname: Gomolka
authors.affiliation: Central Laboratory of Experimental Animals, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Robert
authors.initials: R
authors.lastname: Wrzesien
authors.affiliation: Department of Experimental and Clinical Physiology, Laboratory of Centre for Preclinical Research, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Tymoteusz
authors.initials: T
authors.lastname: Zera
authors.affiliation: Department of Pathology, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Gornicka
authors.affiliation: Chair and Department of Cardiology, Hypertension and Internal Medicine, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Marek
authors.initials: M
authors.lastname: Kuch
mesh_terms: D000818:Animals; D000075244:Apelin Receptors; D001794:Blood Pressure / Q000502:physiology; D059305:Diet, High-Fat / Q000009:adverse effects*; D006333:Heart Failure / Q000209:etiology / Q000378:metabolism*; D006339:Heart Rate / Q000502:physiology*; D006352:Heart Ventricles / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000502:physiology*; D008297:Male; D009203:Myocardial Infarction / Q000209:etiology / Q000378:metabolism*; D012333:RNA, Messenger / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D043562:Receptors, G-Protein-Coupled / Q000502:physiology*
chemical_list: D000075244:Apelin Receptors; C491284:Aplnr protein, rat; D036341:Intercellular Signaling Peptides and Proteins; D012333:RNA, Messenger; D043562:Receptors, G-Protein-Coupled; C495199:apelin-13 peptide
keywords: APJ receptor; apelin; blood pressure; high fat diet; post-infarct heart failure