title: Poly(2-ethyloxazoline) as matrix for highly active electrospun enzymes in organic solvents.
abstract: Nanofibers are advantageous carriers for biocatalysts, because they show lower diffusion limitations due to their high surface/volume ratio. Only a few samples are known where enzymes are directly spun into nanofibers, mostly because there are not many suited polymer carriers. In this study, poly(2-ethyloxazoline) (PEtOx) was explored regarding its usefulness to activate various enzymes in organic solvents by directly electrospinning them from aqueous solutions containing the polymer. It was found that the concentration of PEtOx in the spinning solution and also the swellability of the fibers play a great role in the activity of the enzymes in organic solvents. Using electrospun lipase B from Candida antarctica (CaLB) under optimized conditions revealed a higher carrier activity than the commercial Novozyme 435 with 10 times less immobilized protein. The electrospinning of PEtOx/CaLB fibers onto a stirrer is used to realize a biocatalytic stirrer for organic solvents. Biotechnol. Bioeng. 2017;114: 39-45. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Bio- and Chemical Engineering, TU Dortmund, Emil-Figge-Str. 66, D-44227 Dortmund, Germany.
authors.forename: Ramona
authors.initials: R
authors.lastname: Plothe
authors.affiliation: Department of Bio- and Chemical Engineering, TU Dortmund, Emil-Figge-Str. 66, D-44227 Dortmund, Germany.
authors.forename: Ina
authors.initials: I
authors.lastname: Sittko
authors.affiliation: Department of Bio- and Chemical Engineering, TU Dortmund, Emil-Figge-Str. 66, D-44227 Dortmund, Germany.
authors.forename: Franziska
authors.initials: F
authors.lastname: Lanfer
authors.affiliation: Department of Bio- and Chemical Engineering, TU Dortmund, Emil-Figge-Str. 66, D-44227 Dortmund, Germany.
authors.forename: Maximilian
authors.initials: M
authors.lastname: Fortmann
authors.affiliation: Department of Bio- and Chemical Engineering, TU Dortmund, Emil-Figge-Str. 66, D-44227 Dortmund, Germany.
authors.forename: Meike
authors.initials: M
authors.lastname: Roth
authors.affiliation: Department of Bio- and Chemical Engineering, TU Dortmund, Emil-Figge-Str. 66, D-44227 Dortmund, Germany.
authors.forename: Vivien
authors.initials: V
authors.lastname: Kolbach
authors.affiliation: Department of Bio- and Chemical Engineering, TU Dortmund, Emil-Figge-Str. 66, D-44227 Dortmund, Germany.
authors.forename: Joerg C
authors.initials: JC
authors.lastname: Tiller
mesh_terms: D055162:Biocatalysis; D019149:Bioreactors; D055664:Electrochemical Techniques / Q000379:methods*; D004798:Enzymes / Q000737:chemistry* / Q000378:metabolism*; D005656:Fungal Proteins; D008049:Lipase; D008956:Models, Chemical; D010080:Oxazoles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry
chemical_list: D004798:Enzymes; D005656:Fungal Proteins; D010080:Oxazoles; D011108:Polymers; D012997:Solvents; C071857:polyethyloxazoline; D008049:Lipase; C431349:lipase B, Candida antarctica
keywords: biocatalysis; electrospinning; enzymes; poly(2-ethyloxazoline); polymer nanofibers