title: Genomic Characterizations of a Newcastle Disease Virus Isolated from Ducks in Live Bird Markets in China.
abstract: One class I Newcastle disease virus (NDV), designated as duck/Guangxi/1261/2015 (GX1261), was isolated from asymptomatic ducks in live bird markets (LBM) from southern China during the national active surveillance for NDVs in 2015. The complete genome length of GX1261 isolate was 15,198 nucleotides with the gene order of 3'-NP-P-M-F-HN-L-5'. The motif at the cleavage site of F protein was 112ERQER/L117, which was typical of low virulence NDV. Several mutations were identified in the functional domains of F and HN proteins, including fusion peptide, heptad repeat region, transmembrane domains and neutralizing epitopes. Phylogenetic analysis based on the complete F gene revealed that the isolate was clustered into sub-genotype 1c in class I, and showed a high level of similarity with the strains isolated from waterfowl in the United States of America. This is the first report of this kind of virus in the mainland of China. These results demonstrated that GX1261-like viruses might exist in asymptomatic waterfowl, and remain undetected or unidentified. Thus, more investigation needs to be done in order to identify the source of the virus. This study revealed the genetic and phylogenetic characteristics of GX1261 isolate and could help us to better understand the epidemiological context of class I NDV in China.
authors.affiliation: OIE Reference Laboratory for Newcastle Disease, China Animal Health and Epidemiology Center, Qingdao 266032, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Wang
authors.affiliation: OIE Reference Laboratory for Newcastle Disease, China Animal Health and Epidemiology Center, Qingdao 266032, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Lv
authors.affiliation: OIE Collaborating Centre for Veterinary Epidemiology and Public Health, China Animal Health and Epidemiology Center, Qingdao 266032, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: OIE Reference Laboratory for Newcastle Disease, China Animal Health and Epidemiology Center, Qingdao 266032, China.
authors.forename: Dongxia
authors.initials: D
authors.lastname: Zheng
authors.affiliation: OIE Reference Laboratory for Newcastle Disease, China Animal Health and Epidemiology Center, Qingdao 266032, China.
authors.forename: Yunling
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: DM Castellan International Veterinary Consulting, Niagara Falls, Canada.
authors.forename: David
authors.initials: D
authors.lastname: Castellan
authors.affiliation: OIE Reference Laboratory for Newcastle Disease, China Animal Health and Epidemiology Center, Qingdao 266032, China.
authors.forename: Hualei
authors.initials: H
authors.lastname: Liu
authors.affiliation: OIE Reference Laboratory for Newcastle Disease, China Animal Health and Epidemiology Center, Qingdao 266032, China.
authors.forename: Zhiliang
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D001717:Birds; D002681:China; D004372:Ducks / Q000821:virology*; D016679:Genome, Viral / Q000235:genetics; D009521:Newcastle Disease / Q000821:virology*; D009522:Newcastle disease virus / Q000472:pathogenicity*; D012367:RNA, Viral / Q000235:genetics
chemical_list: D012367:RNA, Viral