title: Extraction, Identification, and Quantification of Histones from Small Quantities of Specific Brain Tissue.
abstract: Histone posttranslational modifications (PTMs), by their action on the chromatin state, play a central role in the regulation of gene expression. The discovery that some PTMs in the brain are dynamically regulated by experience and environmental factors makes them an important subject for the study of plasticity changes in learning and memory, addiction, and psychiatric disorders. Current histone isolation protocols, however, require large amounts of tissue, which limits their application for analyzing small tissue samples from a specific brain region. We describe here a step-by-step protocol for histone extraction and isolation from 1 mm(3) of tissue from brain punches, which allows reproducible and reliable results for histone PTM identification and quantification without losing anatomical precision. © 2016 by John Wiley & Sons, Inc.
authors.affiliation: Department of Cognitive Neuroscience, Radboud University Medical Center, Nijmegen, The Netherlands.|Donders Institute for Brain, Cognition, and Behaviour, Nijmegen, The Netherlands.|Department of Neuroscience, Section Anatomy, University Medical Center Groningen, Groningen, The Netherlands.
authors.forename: Hassiba
authors.initials: H
authors.lastname: Beldjoud
authors.affiliation: Department of Neuroscience, Section Anatomy, University Medical Center Groningen, Groningen, The Netherlands.
authors.forename: Fany
authors.initials: F
authors.lastname: Messanvi
authors.affiliation: Department of Cognitive Neuroscience, Radboud University Medical Center, Nijmegen, The Netherlands.|Donders Institute for Brain, Cognition, and Behaviour, Nijmegen, The Netherlands.|Department of Human Genetics, Radboud University Medical Center, Nijmegen, The Netherlands.
authors.forename: Nael
authors.initials: N
authors.lastname: Nadif Kasri
authors.affiliation: Department of Cognitive Neuroscience, Radboud University Medical Center, Nijmegen, The Netherlands.|Donders Institute for Brain, Cognition, and Behaviour, Nijmegen, The Netherlands.
authors.forename: Benno
authors.initials: B
authors.lastname: Roozendaal
mesh_terms: D000107:Acetylation; D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology*; D002843:Chromatin / Q000473:pathology*; D006657:Histones / Q000378:metabolism*; D006801:Humans; D008568:Memory / Q000502:physiology; D011499:Protein Processing, Post-Translational / Q000502:physiology; D015203:Reproducibility of Results
chemical_list: D002843:Chromatin; D006657:Histones
keywords: acetylation; epigenetics; hippocampus; histone; insular cortex; methylation; phosphorylation; posttranslational modification; western blotting