title: Inherent properties not conserved in other tenuiviruses increase priming and realignment cycles during transcription of Rice stripe virus.
abstract: Two tenuiviruses Rice stripe virus (RSV) and Rice grassy stunt virus (RGSV) were found to co-infect rice with the same reovirus Rice ragged stunt virus (RRSV). During the co-infection, both tenuiviruses recruited 10-21 nucleotides sized capped-RNA leaders from the RRSV. A total of 245 and 102 RRSV-RGSV and RRSV-RSV chimeric mRNA clones, respectively, were sequenced. An analysis of the sequences suggested a scenario consistent with previously reported data on related viruses, in which capped leader RNAs having a 3' end complementary to the viral template are preferred and upon base pairing the leaders prime processive transcription directly or after one to several cycles of priming and realignment (repetitive prime-and-realign). Interestingly, RSV appeared to have a higher tendency to use repetitive prime-and-realign than RGSV even with the same leader derived from the same RRSV RNA. Combining with relevant data reported previously, this points towards an intrinsic feature of RSV.
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
authors.forename: Guihong
authors.initials: G
authors.lastname: Xiong
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Qiu
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China. Electronic address: duzhenguo1228@163.com.
authors.forename: Zhenguo
authors.initials: Z
authors.lastname: Du
authors.affiliation: Laboratory of Virology, Wageningen University and Research Centre, Wageningen, The Netherlands.
authors.forename: Richard
authors.initials: R
authors.lastname: Kormelink
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
authors.forename: Luping
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
authors.forename: Xinlun
authors.initials: X
authors.lastname: Ding
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Yang
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
authors.forename: Songbai
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China. Electronic address: wuzujian@126.com.
authors.forename: Zujian
authors.initials: Z
authors.lastname: Wu
mesh_terms: D020121:5' Untranslated Regions; D001483:Base Sequence; D060085:Coinfection; D019143:Evolution, Molecular; D012275:Oryza / Q000821:virology*; D010935:Plant Diseases / Q000821:virology; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral; D029364:Tenuivirus / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D020121:5' Untranslated Regions; D012333:RNA, Messenger; D012367:RNA, Viral
keywords: Cap-snatching; Co-infection; Plant-infecting reovirus; Prime-and-realign; Tenuivirus