title: Glycolaldehyde Production from Ethylene Glycol with Immobilized Alcohol Oxidase and Catalase.
abstract: An enzymatic method for glycolaldehyde production from ethylene glycol was investigated using immobilized alcohol oxidase and catalase. Those enzymes were immobilized onto Chitopearl BCW 3501. When only alcohol oxidase was immobilized onto it, the apparent activity was 190 units/g in wet gel using methanol as the substrate. Tris-HCl buffer (1.5 M; pH 9.0) was selected based on a high stability of glycolaldehyde and a low production of glyoxal as a by-product. Under the optimum conditions, 0.97 M glycolaldehyde was formed from 1.0 M ethylene glycol and the ratio of glyoxal to glycolaldehyde was less than 1%.
authors.affiliation: a Department of Bioresources Science, Faculty of Agriculture, Kochi University.
authors.forename: H
authors.initials: H
authors.lastname: Ukeda
authors.affiliation: a Department of Bioresources Science, Faculty of Agriculture, Kochi University.
authors.forename: T
authors.initials: T
authors.lastname: Ishii
authors.affiliation: a Department of Bioresources Science, Faculty of Agriculture, Kochi University.
authors.forename: M
authors.initials: M
authors.lastname: Sawamura
authors.affiliation: b Research and Development Division, Amano Pharmaceutical Co., Ltd.
authors.forename: K
authors.initials: K
authors.lastname: Isobe
keywords: alcohol oxidase; catalase; ethylene glycol; glycolaldehyde; immobilized enzyme