title: Biological and Immunogenicity Property of IgY Anti S. mutans ComD.
abstract: OBJECTIVE
  This study aims to elucidate the effect of IgY anti ComD on the biological properties of Streptococcus mutans. (S. mutans) ComD is an interspecies quorum-sensing signaling receptor that plays an important role in biofilm formation by S. mutans.
  
  
  METHODS
  Egg yolk IgY was produced by the immunization of chickens with a DNA vaccine containing the ComD DNA coding region. We evaluated the effect of the antibody on biofilm formation by S. mutans isolated from subjects with or without dental caries. We also assessed the immunoreactivity of the antibody against all isolates, and analyzed the protein profile of S. mutans by SDS-PAGE.
  
  
  RESULTS
  The ComD antibody was successfully induced in the hens' eggs. It inhibited biofilm formation by all S. mutans isolates. In addition, the expression of some protein bands was affected after exposure to the antibody.
  
  
  CONCLUSIONS
  IgY anti-S. mutans ComD reduces biofilm formation by this bacterium and alters the protein profile of S. mutans.
authors.affiliation: Department of of Oral Biology, Faculty of Dentistry, Oral Sciences Research Center, Universitas Indonesia, Jakarta, Indonesia.
authors.forename: E W
authors.initials: EW
authors.lastname: Bachtiar
authors.affiliation: Department of of Oral Biology, Faculty of Dentistry, Oral Sciences Research Center, Universitas Indonesia, Jakarta, Indonesia.
authors.forename: B M
authors.initials: BM
authors.lastname: Bachtiar
authors.affiliation: Faculty of Veterinary Bogor, Institute of Agriculture, Bogor, Indonesia.
authors.forename: R D
authors.initials: RD
authors.lastname: Soejoedono
authors.affiliation: Faculty of Veterinary Bogor, Institute of Agriculture, Bogor, Indonesia.
authors.forename: I W
authors.initials: IW
authors.lastname: Wibawan
authors.affiliation: Department of of Oral Biology, Faculty of Dentistry, Oral Sciences Research Center, Universitas Indonesia, Jakarta, Indonesia.
authors.forename: A
authors.initials: A
authors.lastname: Afdhal
keywords: Biofilm; Caries; Streptococcus mutans; Vaccine