title: Different expression and subcellular localization of Phosphoinositide-specific Phospholipase C enzymes in differently polarized macrophages.
abstract: Macrophages' phenotypic and functional diversity depends on differentiating programs related to local environmental factors. Recent interest was deserved to the signal transduction pathways acting in macrophage polarization, including the phosphoinositide (PI) system and related phospholipase C (PLC) family of enzymes. The expression panel of PLCs and the subcellular localization differs in quiescent cells compared to the pathological counterpart. We analyzed the expression of PLC enzymes in unpolarized (M0), as well as in M1 and M2 macrophages to list the expressed isoforms and their subcellular localization. Furthermore, we investigated whether inflammatory stimulation modified the basal panel of PLCs' expression and subcellular localization. All PLC enzymes were detected within both M1 and M2 cells, but not in M0 cells. M0, as well as M1 and M2 cells own a specific panel of expression, different for both genes' mRNA expression and intracellular localization of PLC enzymes. The panel of PLC genes' expression and PLC proteins' presence slightly changes after inflammatory stimulation. PLC enzymes might play a complex role in macrophages during inflammation and probably also during polarization.
authors.affiliation: Department of Medico-Surgical Sciences and Biotechnology, Polo Pontino - Sapienza University, Latina, Italy.
authors.forename: Tania
authors.initials: T
authors.lastname: Di Raimo
authors.affiliation: Department of Medico-Surgical Sciences and Biotechnology, Polo Pontino - Sapienza University, Latina, Italy.
authors.forename: Martina
authors.initials: M
authors.lastname: Leopizzi
authors.affiliation: Department of Medico-Surgical Sciences and Biotechnology, Polo Pontino - Sapienza University, Latina, Italy.
authors.forename: Giorgio
authors.initials: G
authors.lastname: Mangino
authors.affiliation: Department of Medico-Surgical Sciences and Biotechnology, Polo Pontino - Sapienza University, Latina, Italy.
authors.forename: Carlo Della
authors.initials: CD
authors.lastname: Rocca
authors.affiliation: Department of Medico-Surgical Sciences and Biotechnology, Polo Pontino - Sapienza University, Latina, Italy.
authors.forename: Rita
authors.initials: R
authors.lastname: Businaro
authors.affiliation: Department Sensory Organs, Faculty of Medicine and Dentistry, Policlinico Umberto I, Sapienza University of Rome, Viale dell'Università 33, 00100, Rome, Italy.
authors.forename: Lucia
authors.initials: L
authors.lastname: Longo
authors.affiliation: Department Sensory Organs, Faculty of Medicine and Dentistry, Policlinico Umberto I, Sapienza University of Rome, Viale dell'Università 33, 00100, Rome, Italy. ritalovasco@hotmail.it.
authors.forename: Vincenza Rita
authors.initials: VR
authors.lastname: Lo Vasco
keywords: Gene expression; Inflammation; LPS; M0; M1; M2; Macrophage polarization; Nanotube; PLC; Podosome; Post-transcriptional regulation; Signal transduction