title: Biosynthetic Relationship between Acutumine and Dechloroacutumine in Menispermum dauricum Root Cultures.
abstract: The biosynthetic relationship between acutumine 1 and dechloroacutumine 2 was studied using (13)C-labeled tyrosine and (3)H-labeled 2 as tracers. (13)C-NMR spectra of (13)C-labeled 1 and 2 showed that the alkaloids, each composed of two molecules of tyrosine, are derived from the same biosynthetic pathway. Feeding Menispermum dauricum (Menispermaceae) roots, cultured in a chloride-enriched medium, with (3)H-labeled 2 demonstrated that 1 is the only alkaloid metabolite of 2. Conversion (5%) of the exogenously applied 2, taken up by the roots, into 1 showed that 2 is the precursor of 1. Incomplete conversion of 2 into 1 suggests accumulation of the exogenously applied 2 in cell organelles and/or compartmentation of the enzymes involved in the biosynthesis of 1.
authors.affiliation: a Arid Land Research Center, Tottori University.
authors.forename: H A
authors.initials: HA
authors.lastname: Babiker
authors.affiliation: a Arid Land Research Center, Tottori University.
authors.forename: Y
authors.initials: Y
authors.lastname: Sugimoto
authors.affiliation: a Arid Land Research Center, Tottori University.
authors.forename: T
authors.initials: T
authors.lastname: Saisho
authors.affiliation: a Arid Land Research Center, Tottori University.
authors.forename: S
authors.initials: S
authors.lastname: Inanaga
authors.affiliation: b Nitto Technical Information Center Co., Ltd.
authors.forename: M
authors.initials: M
authors.lastname: Hashimoto
authors.affiliation: c Graduate School of Biological Science, Nara Institute of Science and Technology.
authors.forename: A
authors.initials: A
authors.lastname: Isogai
keywords: Menispermum dauricum; acutumine; biosynthesis; dechloroacutumine; root cultures