title: The role of oncostatin M regulates osteoblastic differentiation of dental pulp stem cells through STAT3 pathway.
abstract: Dental pulp stem cells (DPSCs) are a type of mesenchymal stem cells, which have the self-renewal and multi-lineage differentiation potential, including chondrocytes, adipocytes, neural cells and osteoblasts. So they play a significant role in pulp repair and bone regeneration. Oncostatin M (OSM), one of the IL-6 family cytokines, inhibits adipogenic differentiation and stimulates osteogenic differentiation of human bone marrow mesenchymal stem cells. However, the effect of OSM on DPSCs is unclear. We found that OSM induced osteogenic differentiation of DPSCs, promoting matrix mineralization as measured by Alizarin Red S staining. OSM also increased expression of osteogenesis-associated gene products Alkaline phosphatase, Bone morphogenetic protein 2 (BMP2), Runt-related transcription factor 2 and Osteocalcin (OCN) as assessed by immunoblotting. We also found that OSM activated the Signal Transducer And Activator Of Transcription 3 (STAT3) pathway during the osteogenic differentiation of DPSCs. Blocking the osteogenic differentiation by silencing of STAT3 can significantly inhibit OSM-induced osteogenic differentiation of DPSCs and the expression of related genes, furthermore matrix mineralization was also suppressed. In summary, OSM promotes osteoblastic differentiation of DPSCs and osteogenesis-related genes expression through the JAK3/STAT3 signaling pathway which may be useful for the autologous transplantation of DPSCs.
authors.affiliation: Department of Stomatology, Affiliated Hospital of Nantong University, Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong, 226000, China.
authors.forename: Xingmei
authors.initials: X
authors.lastname: Feng
authors.affiliation: Department of Stomatology, Affiliated Hospital of Nantong University, Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong, 226000, China.
authors.forename: Shuling
authors.initials: S
authors.lastname: Shen
authors.affiliation: Department of Stomatology, Affiliated Hospital of Nantong University, Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong, 226000, China.
authors.forename: Peipei
authors.initials: P
authors.lastname: Cao
authors.affiliation: Department of Mathematics, Nanjing University of Aeronautics and Astronautics, Nanjing, 210016, China.
authors.forename: Linhe
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Department of Stomatology, Affiliated Hospital of Nantong University, Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong, 226000, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Stomatology, Affiliated Hospital of Nantong University, Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong, 226000, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Zheng
authors.affiliation: Department of Stomatology, Affiliated Hospital of Nantong University, Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong, 226000, China. fengguijuan2013@126.com.
authors.forename: Guijuan
authors.initials: G
authors.lastname: Feng
authors.affiliation: Department of Pathogen Biology, Medical College, Nantong University, Nantong, 226000, China. zdm060608@163.com.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Zhang
keywords: Dental pulp stem cells (DPSCs); Janus kinase 3 (JAK3); OSM; Osteogenic differentiation; STAT3