title: Resonance Raman spectroscopic study of the interaction between Co(II)rrinoids and the ATP:corrinoid adenosyltransferase PduO from Lactobacillus reuteri.
abstract: The human-type ATP:corrinoid adenosyltransferase PduO from Lactobacillus reuteri (LrPduO) catalyzes the adenosylation of Co(II)rrinoids to generate adenosylcobalamin (AdoCbl) or adenosylcobinamide (AdoCbi(+)). This process requires the formation of "supernucleophilic" Co(I)rrinoid intermediates in the enzyme active site which are properly positioned to abstract the adeonsyl moiety from co-substrate ATP. Previous magnetic circular dichroism (MCD) spectroscopic and X-ray crystallographic analyses revealed that LrPduO achieves the thermodynamically challenging reduction of Co(II)rrinoids by displacing the axial ligand with a non-coordinating phenylalanine residue to produce a four-coordinate species. However, relatively little is currently known about the interaction between the tetradentate equatorial ligand of Co(II)rrinoids (the corrin ring) and the enzyme active site. To address this issue, we have collected resonance Raman (rR) data of Co(II)rrinoids free in solution and bound to the LrPduO active site. The relevant resonance-enhanced vibrational features of the free Co(II)rrinoids are assigned on the basis of rR intensity calculations using density functional theory to establish a suitable framework for interpreting rR spectral changes that occur upon Co(II)rrinoid binding to the LrPduO/ATP complex in terms of structural perturbations of the corrin ring. To complement our rR data, we have also obtained MCD spectra of Co(II)rrinoids bound to LrPduO complexed with the ATP analogue UTP. Collectively, our results provide compelling evidence that in the LrPduO active site, the corrin ring of Co(II)rrinoids is firmly locked in place by several amino acid side chains so as to facilitate the dissociation of the axial ligand.
authors.affiliation: Department of Chemistry, Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon, 34141, Republic of Korea. kiyoung.park@kaist.ac.kr.
authors.forename: Kiyoung
authors.initials: K
authors.lastname: Park
authors.affiliation: Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, NM, 88003, USA.
authors.forename: Paola E
authors.initials: PE
authors.lastname: Mera
authors.affiliation: Department of Microbiology, University of Georgia, Athens, GA, 30602, USA.
authors.forename: Jorge C
authors.initials: JC
authors.lastname: Escalante-Semerena
authors.affiliation: Department of Chemistry, University of Wisconsin-Madison, Madison, WI, 53706, USA. brunold@chem.wisc.edu.
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Brunold
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry* / Q000378:metabolism; D000445:Aldehyde Oxidoreductases / Q000737:chemistry* / Q000378:metabolism; D003035:Cobalt / Q000737:chemistry* / Q000378:metabolism; D052200:Limosilactobacillus reuteri / Q000201:enzymology*; D011789:Quantum Theory; D013059:Spectrum Analysis, Raman; D014805:Vitamin B 12 / Q000031:analogs & derivatives / Q000737:chemistry* / Q000378:metabolism; D001426:Bacterial Proteins
chemical_list: D000255:Adenosine Triphosphate; D000445:Aldehyde Oxidoreductases; D003035:Cobalt; D014805:Vitamin B 12; C588134:coenzyme A-acylating propionaldehyde dehydrogenase, Lactobacillus reuteri; D001426:Bacterial Proteins
keywords: ATP:corrinoid adenosyltransferase; Adenosylcobalamin; Density functional theory; Resonance Raman spectroscopy