title: Ghrelin inhibits high glucose-induced 16HBE cells apoptosis by regulating Wnt/β-catenin pathway.
abstract: Ghrelin has a protective effect on diabetes and its complications. To expound its probable molecular mechanisms, we investigated the effects of ghrelin on high glucose (HG)-induced cell apoptosis and intracellular signaling pathways in cultured human bronchial epithelial cells (16HBE). In this study, we firstly came to conclusion that HG-induced 16HBE apoptosis was significantly inhibited by co-treatment of ghrelin. The molecular mechanism of ghrelin-induced protective effects for lungs is still not understood. We reported here for the first time that ghrelin can not only eliminate apoptosis of 16HBE, but also regulate the disordered cell cycle caused by HG. We speculated here that ghrelin inhibits the apoptosis of 16HBE by regulating the abnormal cell cycle to some extent. The mechanism may be that ghrelin up-regulates the expression of cyclin D1 via regulating Wnt/β-catenin pathway, which has an intimate relationship with lung diseases. These results suggested the possible role of ghrelin in treating diabetic lung diseases, especially in view of its low toxicity in humans.
authors.affiliation: Department of Respiratory Medicine, The Second Affiliated Hospital of Shanxi Medical University, Shanxi, 030001, China. Electronic address: liuxy1985@foxmail.com.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Chongqing Three Gorges Medical College, Chongqing, 404120, China.
authors.forename: Dilong
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Hepatobiliary Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Zhongjun
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Department of Histology and Embryology, Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Respiratory Medicine, The Second Affiliated Hospital of Shanxi Medical University, Shanxi, 030001, China.
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Respiratory Medicine, The Second Affiliated Hospital of Shanxi Medical University, Shanxi, 030001, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Respiratory Medicine, The Second Affiliated Hospital of Shanxi Medical University, Shanxi, 030001, China.
authors.forename: Tanzhen
authors.initials: T
authors.lastname: Liu
mesh_terms: D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D054439:Ghrelin / Q000008:administration & dosage / Q000493:pharmacokinetics*; D005947:Glucose / Q000008:administration & dosage*; D006801:Humans; D020545:Respiratory Mucosa / Q000187:drug effects / Q000378:metabolism*; D060449:Wnt Signaling Pathway / Q000187:drug effects / Q000502:physiology*
chemical_list: D054439:Ghrelin; D005947:Glucose
keywords: 16HBE; Cell cycle; Ghrelin; High glucose (HG); Wnt/β-catenin pathway