title: Purification of Pomacea canaliculata α-Fucosidase Isoforms with Different Thermostabilities.
abstract: Two isoforms, F1 (pI 4.7) and F2 (pI 4.9), of α-fucosidase were purified from the viscera of Pomacea canaliculata. The thermostability of F1 was higher than that of F2. Both were 260-kDa proteins containing polypeptides (55 and 52 kDa) and sugar. There were small differences between F1 and F2 in the optimum conditions for the enzyme reaction and pH stability.
authors.affiliation: a Laboratory of Protein Chemistry and Engineering, Graduate School of Genetic Resources Technology, Kyushu University.
authors.forename: K
authors.initials: K
authors.lastname: Hirata
authors.affiliation: a Laboratory of Protein Chemistry and Engineering, Graduate School of Genetic Resources Technology, Kyushu University.
authors.forename: Y
authors.initials: Y
authors.lastname: Aso
authors.affiliation: a Laboratory of Protein Chemistry and Engineering, Graduate School of Genetic Resources Technology, Kyushu University.
authors.forename: S
authors.initials: S
authors.lastname: Yasuda
authors.affiliation: a Laboratory of Protein Chemistry and Engineering, Graduate School of Genetic Resources Technology, Kyushu University.
authors.forename: M
authors.initials: M
authors.lastname: Ishiguro
keywords: Pomacea canaliculata; apple snail; isoform; α-fucosidase