title: Anti-viral Effect of Bifidobacterium adolescentis against Noroviruses.
abstract: This study aims to investigate the effect of Bifidobacterium adolescentis against noroviruses (NoVs). Murine norovirus-1 (MNV-1) used as a surrogate was detected by plaque assay and RT-qPCR. Human NoV virus like particles (VLPs) were detected by cell-binding assay. It was shown that the presence of B. adolescentis could inhibit the multiplication of MNV-1 on RAW 264.7 cells within 48 h of co-incubation period at 37°C. This inhibition did not occur at the viral binding stage, as no difference was observed in MNV-1 genomic copies collected from washed RAW 264.7 cells without and with B. adolescentis after co-incubation for 1 h at room temperature. Meanwhile, the presence of B. adolescentis decreased the binding of human NoV GI.1 VLPs to both Caco-2 cells and HT-29 cells, while no reduction was induced for the binding of human NoV GII.4 VLPs to Caco-2 cells.
authors.affiliation: Laboratory of Food Microbiology and Food Preservation, Faculty of Bioscience Engineering, Ghent University Ghent, Belgium.
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.affiliation: UMR 892, Institut National de la Santé et de la Recherche MédicaleNantes, France; UMR 6299, Centre National de la Recherche ScientifiqueNantes, France; Faculty of Medicine, University of NantesNantes, France; Nantes University HospitalNantes, France.
authors.forename: Adrien
authors.initials: A
authors.lastname: Breiman
authors.affiliation: UMR 892, Institut National de la Santé et de la Recherche MédicaleNantes, France; UMR 6299, Centre National de la Recherche ScientifiqueNantes, France; Faculty of Medicine, University of NantesNantes, France.
authors.forename: Jacques
authors.initials: J
authors.lastname: le Pendu
authors.affiliation: Laboratory of Food Microbiology and Food Preservation, Faculty of Bioscience Engineering, Ghent University Ghent, Belgium.
authors.forename: Mieke
authors.initials: M
authors.lastname: Uyttendaele
keywords: Bifidobacterium adolescentis; Norovirus; mechanisms; probiotics