title: High-level Expression of a Recombinant Thermostable Phytase in Bacillus subtilis.
abstract: An efficient expression system was developed in Bacillus subtilis for the large scale production of phytase. The phytase gene with a native promoter derived from Bacillus amyloliquefaciens was cloned in the Bacillus expression vector pJH27 under a strong BJ27 promoter and its expression was optimized. The expression of the phytase gene occurred during late exponential growth and the extracellular phytase production was 2.0 units/ml, which constituted over 90% of the total protein. The yield was 100-fold higher than wild type Bacillus amyloliquefaciens DS11.
authors.affiliation: a Microbial enzyme RU, Korea Research Institute of Bioscience & Biotechnology (KRIBB).
authors.forename: Y O
authors.initials: YO
authors.lastname: Kim
authors.affiliation: a Microbial enzyme RU, Korea Research Institute of Bioscience & Biotechnology (KRIBB).
authors.forename: J K
authors.initials: JK
authors.lastname: Lee
authors.affiliation: a Microbial enzyme RU, Korea Research Institute of Bioscience & Biotechnology (KRIBB).
authors.forename: B C
authors.initials: BC
authors.lastname: Oh
authors.affiliation: a Microbial enzyme RU, Korea Research Institute of Bioscience & Biotechnology (KRIBB).
authors.forename: T K
authors.initials: TK
authors.lastname: Oh
keywords: Bacillus subtilis; overexpression; phytase; phytate