title: Epithelial-Mesenchymal Transition in Chronic Rhinosinusitis: Differences Revealed Between Epithelial Cells from Nasal Polyps and Inferior Turbinates.
abstract: The pathogenesis of chronic rhinosinusitis (CRS) remains unclear to date. The tissue remodeling in nasal polyps may be the result of inflammatory mediators and may involve epithelial-mesenchymal transition (EMT) and EMT-associated features such as cell motility in nasal epithelial cells (NECs). We determined whether NEC in nasal polyps of CRS already display features of EMT in vivo or respond with EMT to growth factor stimulation in vitro. Nasal polyp tissues expressed both epithelial and mesenchymal markers. Primary NEC from inferior turbinates and nasal polyps responded to the EMT-inducing agents transforming growth factor (TGF)-β1 and epidermal growth factor (EGF) with different expression patterns of EMT markers (E-cadherin, N-cadherin, Snail, Slug, Twist), however, only NEC from nasal polyps were susceptible to TGF-β1 and EGF-dependent cell migration. Our data suggest that a partial EMT is associated with the pathogenesis of nasal polyps in CRS patients. Furthermore, we show for the first time that epithelial cells from both nasal polyps and inferior turbinates were able to undergo an EMT-like process following exposure to TGF-β1 or EGF in vitro but that only NEC from nasal polyps responded with enhanced cell motility. Our data suggest that NEC from CRS patients have undergo partial EMT and that this process may be involved in the pathogenesis of CRS.
authors.affiliation: Department of Otorhinolaryngology, University Hospital Schleswig-Holstein, Campus Lübeck, Ratzeburger Allee 160, 23538, Lübeck, Germany. Michael.Koennecke@uksh.de.
authors.forename: Michael
authors.initials: M
authors.lastname: Könnecke
authors.affiliation: Department of Otorhinolaryngology, University Hospital Schleswig-Holstein, Campus Lübeck, Ratzeburger Allee 160, 23538, Lübeck, Germany.
authors.forename: Maike
authors.initials: M
authors.lastname: Burmeister
authors.affiliation: Department of Otorhinolaryngology, University Hospital Schleswig-Holstein, Campus Lübeck, Ratzeburger Allee 160, 23538, Lübeck, Germany.
authors.forename: Ralph
authors.initials: R
authors.lastname: Pries
authors.affiliation: Department of Otorhinolaryngology, University Hospital Schleswig-Holstein, Campus Lübeck, Ratzeburger Allee 160, 23538, Lübeck, Germany.
authors.forename: Robert
authors.initials: R
authors.lastname: Böscke
authors.affiliation: Department of Otorhinolaryngology, University Hospital Schleswig-Holstein, Campus Lübeck, Ratzeburger Allee 160, 23538, Lübeck, Germany.
authors.forename: Karl-Ludwig
authors.initials: KL
authors.lastname: Bruchhage
authors.affiliation: First Department of Medicine, University Hospital Schleswig-Holstein, Campus Lübeck, Lübeck, Germany.
authors.forename: Hendrik
authors.initials: H
authors.lastname: Ungefroren
authors.affiliation: Center for Rhinology and Allergology, Wiesbaden, Germany.
authors.forename: Ludger
authors.initials: L
authors.lastname: Klimek
authors.affiliation: Department of Otorhinolaryngology, University Hospital Schleswig-Holstein, Campus Lübeck, Ratzeburger Allee 160, 23538, Lübeck, Germany.
authors.forename: Barbara
authors.initials: B
authors.lastname: Wollenberg
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002465:Cell Movement; D004815:Epidermal Growth Factor / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism*; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007249:Inflammation; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D012852:Sinusitis / Q000378:metabolism*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D014420:Turbinates / Q000378:metabolism*; D055815:Young Adult
chemical_list: D053773:Transforming Growth Factor beta1; D004815:Epidermal Growth Factor
keywords: CRSwNP; Chronic rhinosinusitis with nasal polyps; EMT; Epithelial–mesenchymal transition; Nasal epithelial cells; Nasal polyps