title: Evaluation of treadmill exercise effect on muscular lipid profiles of diabetic fatty rats by nanoflow liquid chromatography-tandem mass spectrometry.
abstract: We compare comprehensive quantitative profiling of lipids at the molecular level from skeletal muscle tissues (gastrocnemius and soleus) of Zucker diabetic fatty rats and Zucker lean control rats during treadmill exercise by nanoflow liquid chromatography-tandem mass spectrometry. Because type II diabetes is caused by decreased insulin sensitivity due to excess lipids accumulated in skeletal muscle tissue, lipidomic analysis of muscle tissues under treadmill exercise can help unveil the mechanism of lipid-associated insulin resistance. In total, 314 lipid species, including phospholipids, sphingolipids, ceramides, diacylglycerols (DAGs), and triacylglycerols (TAGs), were analyzed to examine diabetes-related lipid species and responses to treadmill exercise. Most lysophospholipid levels increased with diabetes. While DAG levels (10 from the gastrocnemius and 13 from the soleus) were >3-fold higher in diabetic rats, levels of most of these decreased after exercise in soleus but not in gastrocnemius. Levels of 5 highly abundant TAGs (52:1 and 54:3 in the gastrocnemius and 48:2, 50:2, and 52:4 in the soleus) displaying 2-fold increases in diabetic rats decreased after exercise in the soleus but not in the gastrocnemius in most cases. Thus, aerobic exercise has a stronger influence on lipid levels in the soleus than in the gastrocnemius in type 2 diabetic rats.
authors.affiliation: Department of Chemistry, Yonsei University, Seoul, 03722, Korea.
authors.forename: Jong Cheol
authors.initials: JC
authors.lastname: Lee
authors.affiliation: College of Veterinary Medicine, Seoul National University, Seoul, 08826, Korea.|Korea Mouse Phenotyping Center (KMPC), Seoul National University, Seoul, 08826, Korea.
authors.forename: Il Yong
authors.initials: IY
authors.lastname: Kim
authors.affiliation: College of Veterinary Medicine, Seoul National University, Seoul, 08826, Korea.|Korea Mouse Phenotyping Center (KMPC), Seoul National University, Seoul, 08826, Korea.
authors.forename: Yeri
authors.initials: Y
authors.lastname: Son
authors.affiliation: Department of Chemistry, Yonsei University, Seoul, 03722, Korea.
authors.forename: Seul Kee
authors.initials: SK
authors.lastname: Byeon
authors.affiliation: Korea Mouse Phenotyping Center (KMPC), Seoul National University, Seoul, 08826, Korea.|Health and Exercise Science Laboratory, Institute of Sport Science, Seoul National University College of Education, Seoul, 08826, Korea.
authors.forename: Dong Hyun
authors.initials: DH
authors.lastname: Yoon
authors.affiliation: Korea Mouse Phenotyping Center (KMPC), Seoul National University, Seoul, 08826, Korea.|Health and Exercise Science Laboratory, Institute of Sport Science, Seoul National University College of Education, Seoul, 08826, Korea.
authors.forename: Jun Seok
authors.initials: JS
authors.lastname: Son
authors.affiliation: Korea Mouse Phenotyping Center (KMPC), Seoul National University, Seoul, 08826, Korea.|Health and Exercise Science Laboratory, Institute of Sport Science, Seoul National University College of Education, Seoul, 08826, Korea.
authors.forename: Han Sol
authors.initials: HS
authors.lastname: Song
authors.affiliation: Korea Mouse Phenotyping Center (KMPC), Seoul National University, Seoul, 08826, Korea.|Health and Exercise Science Laboratory, Institute of Sport Science, Seoul National University College of Education, Seoul, 08826, Korea.|Institute on Aging, Seoul National University College of Medicine, Seoul, 08826, Korea.
authors.forename: Wook
authors.initials: W
authors.lastname: Song
authors.affiliation: College of Veterinary Medicine, Seoul National University, Seoul, 08826, Korea.|Korea Mouse Phenotyping Center (KMPC), Seoul National University, Seoul, 08826, Korea.
authors.forename: Je Kyung
authors.initials: JK
authors.lastname: Seong
authors.affiliation: Department of Chemistry, Yonsei University, Seoul, 03722, Korea.
authors.forename: Myeong Hee
authors.initials: MH
authors.lastname: Moon
mesh_terms: D000818:Animals; D016022:Case-Control Studies; D002853:Chromatography, Liquid / Q000379:methods; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D008055:Lipids / Q000032:analysis*; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism*; D036103:Nanotechnology; D009765:Obesity / Q000378:metabolism*; D010805:Physical Conditioning, Animal / Q000379:methods*; D051381:Rats; D011924:Rats, Zucker; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D008055:Lipids