title: The Effect of Post-Resistance Exercise Amino Acids on Plasma MCP-1 and CCR2 Expression.
abstract: The recruitment and infiltration of classical monocytes into damaged muscle is critical for optimal tissue remodeling. This study examined the effects of an amino acid supplement on classical monocyte recruitment following an acute bout of lower body resistance exercise. Ten resistance-trained men (24.7 ± 3.4 years; 90.1 ± 11.3 kg; 176.0 ± 4.9 cm) ingested supplement (SUPP) or placebo (PL) immediately post-exercise in a randomized, cross-over design. Blood samples were obtained at baseline (BL), immediately (IP), 30-min (30P), 1-h (1H), 2-h (2H), and 5-h (5H) post-exercise to assess plasma concentrations of monocyte chemoattractant protein 1 (MCP-1), myoglobin, cortisol and insulin concentrations; and expressions of C-C chemokine receptor-2 (CCR2), and macrophage-1 antigen (CD11b) on classical monocytes. Magnitude-based inferences were used to provide inferences on the true effects of SUPP compared to PL. Changes in myoglobin, cortisol, and insulin concentrations were similar between treatments. Compared to PL, plasma MCP-1 was "very likely greater" (98.1% likelihood effect) in SUPP at 2H. CCR2 expression was "likely greater" at IP (84.9% likelihood effect), "likely greater" at 1H (87.7% likelihood effect), "very likely greater" at 2H (97.0% likelihood effect), and "likely greater" at 5H (90.1% likelihood effect) in SUPP, compared to PL. Ingestion of SUPP did not influence CD11b expression. Ingestion of an amino acid supplement immediately post-exercise appears to help maintain plasma MCP-1 concentrations and augment CCR2 expression in resistance trained men.
authors.affiliation: Institute of Exercise Physiology and Wellness, Educational and Human Sciences, University of Central Florida, 12494 University Blvd, Orlando, FL 32816, USA. adam.wells@ucf.edu.
authors.forename: Adam J
authors.initials: AJ
authors.lastname: Wells
authors.affiliation: Institute of Exercise Physiology and Wellness, Educational and Human Sciences, University of Central Florida, 12494 University Blvd, Orlando, FL 32816, USA. Jay.Hoffman@ucf.edu.
authors.forename: Jay R
authors.initials: JR
authors.lastname: Hoffman
authors.affiliation: Institute of Exercise Physiology and Wellness, Educational and Human Sciences, University of Central Florida, 12494 University Blvd, Orlando, FL 32816, USA. adam.jajtner@knights.ucf.edu.
authors.forename: Adam R
authors.initials: AR
authors.lastname: Jajtner
authors.affiliation: Institute of Exercise Physiology and Wellness, Educational and Human Sciences, University of Central Florida, 12494 University Blvd, Orlando, FL 32816, USA. alyssa.varanoske@ucf.edu.
authors.forename: Alyssa N
authors.initials: AN
authors.lastname: Varanoske
authors.affiliation: Institute of Exercise Physiology and Wellness, Educational and Human Sciences, University of Central Florida, 12494 University Blvd, Orlando, FL 32816, USA. david.church@ucf.edu.
authors.forename: David D
authors.initials: DD
authors.lastname: Church
authors.affiliation: Department of Health Professions, Hofstra University, Hempstead, NY 11549, USA. adam.m.gonzalez@hofstra.edu.
authors.forename: Adam M
authors.initials: AM
authors.lastname: Gonzalez
authors.affiliation: Department of Kinesiology, Lipscomb University, Nashville, TN 37204, USA. jeremy.townsend@lipscomb.edu.
authors.forename: Jeremy R
authors.initials: JR
authors.lastname: Townsend
authors.affiliation: Institute of Exercise Physiology and Wellness, Educational and Human Sciences, University of Central Florida, 12494 University Blvd, Orlando, FL 32816, USA. carliegh.boone@ucf.edu.
authors.forename: Carleigh H
authors.initials: CH
authors.lastname: Boone
authors.affiliation: Institute of Exercise Physiology and Wellness, Educational and Human Sciences, University of Central Florida, 12494 University Blvd, Orlando, FL 32816, USA. kayla.baker@ucf.edu.
authors.forename: Kayla M
authors.initials: KM
authors.lastname: Baker
authors.affiliation: Institute of Exercise Physiology and Wellness, Educational and Human Sciences, University of Central Florida, 12494 University Blvd, Orlando, FL 32816, USA. kyle.beyer@ucf.edu.
authors.forename: Kyle S
authors.initials: KS
authors.lastname: Beyer
authors.affiliation: Department of Exercise Science and Sport Management, Kennesaw State University, Kennesaw, GA 30144, USA. gmangine@kennesaw.edu.
authors.forename: Gerald T
authors.initials: GT
authors.lastname: Mangine
authors.affiliation: Institute of Exercise Physiology and Wellness, Educational and Human Sciences, University of Central Florida, 12494 University Blvd, Orlando, FL 32816, USA. leonardo.oliveira@ucf.edu.
authors.forename: Leonardo P
authors.initials: LP
authors.lastname: Oliveira
authors.affiliation: Institute of Exercise Physiology and Wellness, Educational and Human Sciences, University of Central Florida, 12494 University Blvd, Orlando, FL 32816, USA. david.fukuda@ucf.edu.
authors.forename: David H
authors.initials: DH
authors.lastname: Fukuda
authors.affiliation: Institute of Exercise Physiology and Wellness, Educational and Human Sciences, University of Central Florida, 12494 University Blvd, Orlando, FL 32816, USA. jeffrey.stout@ucf.edu.
authors.forename: Jeffrey R
authors.initials: JR
authors.lastname: Stout
mesh_terms: D000284:Administration, Oral; D000328:Adult; D000596:Amino Acids / Q000008:administration & dosage*; D015415:Biomarkers / Q000097:blood; D015992:Body Mass Index; D001835:Body Weight; D039481:CD11b Antigen / Q000235:genetics / Q000378:metabolism; D018932:Chemokine CCL2 / Q000097:blood*; D018592:Cross-Over Studies; D004032:Diet; D019587:Dietary Supplements; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D007328:Insulin / Q000097:blood; D019344:Lactic Acid / Q000097:blood; D008297:Male; D009000:Monocytes / Q000187:drug effects; D009211:Myoglobin / Q000097:blood; D054390:Receptors, CCR2 / Q000235:genetics / Q000378:metabolism*; D055070:Resistance Training; D055815:Young Adult
chemical_list: D000596:Amino Acids; D015415:Biomarkers; C111835:CCL2 protein, human; C515334:CCR2 protein, human; D039481:CD11b Antigen; D018932:Chemokine CCL2; C509295:ITGAM protein, human; D007328:Insulin; D009211:Myoglobin; D054390:Receptors, CCR2; D019344:Lactic Acid; D006854:Hydrocortisone
keywords: C-C chemokine receptor 2 (CCR2); inflammation; macrophage-1 antigen (cluster of differentiation 11b (CD11b)); monocyte chemoattractant protein 1 (MCP-1)