title: The involvement of prostaglandin E2in interleukin-1β evoked anorexia is strain dependent.
abstract: From experiments in mice in which the prostaglandin E2(PGE2) synthesizing enzyme mPGES-1 was genetically deleted, as well as from experiments in which PGE2was injected directly into the brain, PGE2has been implicated as a mediator of inflammatory induced anorexia. Here we aimed at examining which PGE2receptor (EP1-4) that was critical for the anorexic response to peripherally injected interleukin-1β (IL-1β). However, deletion of neither EP receptor in mice, either globally (for EP1, EP2, and EP3) or selectively in the nervous system (EP4), had any effect on the IL-1β induced anorexia. Because these mice were all on a C57BL/6 background, whereas previous observations demonstrating a role for induced PGE2in IL-1β evoked anorexia had been carried out on mice on a DBA/1 background, we examined the anorexic response to IL-1β in mice with deletion of mPGES-1 on a C57BL/6 background and a DBA/1 background, respectively. We confirmed previous findings that mPGES-1 knock-out mice on a DBA/1 background displayed attenuated anorexia to IL-1β; however, mice on a C57BL/6 background showed the same profound anorexia as wild type mice when carrying deletion of mPGES-1, while displaying almost normal food intake after pretreatment with a cyclooxygenase-2 inhibitor. We conclude that the involvement of induced PGE2in IL-1β evoked anorexia is strain dependent and we suggest that different routes that probably involve distinct prostanoids exist by which inflammatory stimuli may evoke an anorexic response and that these routes may be of different importance in different strains of mice.
authors.affiliation: Division of Cell Biology, Department of Clinical and Experimental Medicine, Linköping University, S-581 85 Linköping, Sweden.
authors.forename: Anna
authors.initials: A
authors.lastname: Nilsson
authors.affiliation: Division of Anesthesiology and Intensive Care, Department of Medical and Health Sciences, Linköping University, S-581 85 Linköping, Sweden.
authors.forename: Louise
authors.initials: L
authors.lastname: Elander
authors.affiliation: Division of Experimental Pathology, Department of Clinical and Experimental Medicine, Linköping University, S-581 85 Linköping, Sweden.
authors.forename: Martin
authors.initials: M
authors.lastname: Hallbeck
authors.affiliation: Division of Cell Biology, Department of Clinical and Experimental Medicine, Linköping University, S-581 85 Linköping, Sweden.
authors.forename: Unn
authors.initials: U
authors.lastname: Örtegren Kugelberg
authors.affiliation: Division of Cell Biology, Department of Clinical and Experimental Medicine, Linköping University, S-581 85 Linköping, Sweden.
authors.forename: David
authors.initials: D
authors.lastname: Engblom
authors.affiliation: Division of Cell Biology, Department of Clinical and Experimental Medicine, Linköping University, S-581 85 Linköping, Sweden. Electronic address: anders.blomqvist@liu.se.
authors.forename: Anders
authors.initials: A
authors.lastname: Blomqvist
mesh_terms: D000818:Animals; D000855:Anorexia / Q000378:metabolism*; D002478:Cells, Cultured; D051546:Cyclooxygenase 2 / Q000378:metabolism; D052246:Cyclooxygenase 2 Inhibitors / Q000494:pharmacology*; D015232:Dinoprostone / Q000378:metabolism*; D053583:Interleukin-1beta / Q000378:metabolism*; D019746:Intramolecular Oxidoreductases / Q000187:drug effects / Q000378:metabolism; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D000071876:Prostaglandin-E Synthases / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D052246:Cyclooxygenase 2 Inhibitors; D053583:Interleukin-1beta; D051546:Cyclooxygenase 2; D019746:Intramolecular Oxidoreductases; D000071876:Prostaglandin-E Synthases; D015232:Dinoprostone
keywords: Anorexia; Cyclooxygenase-2; EP receptors; Interleukin-1; Mice; Prostaglandin E(2)