title: Hypouricemic effect of allopurinol are improved by Pallidifloside D based on the uric acid metabolism enzymes PRPS, HGPRT and PRPPAT.
abstract: Allopurinol is a commonly used medication to treat hyperuricemia and its complications. Pallidifloside D, a saponin glycoside constituent from the total saponins of Smilax riparia, had been proved to enhanced hypouricemic effect of allopurinol based on uric acid metabolism enzyme XOD. In this study, we evaluated whether Pallidifloside D (5mg/kg) enhanced hypouricemic effect of allopurinol (5mg/kg) related to others uric acid metabolism enzymes such as PRPS, HGPRT and PRPPAT. We found that, compared with allopurinol alone, the combination of allopurinol and Pallidifloside D significantly up-regulated HGPRT mRNA expression and down-regulated the mRNA expression of PRPS and PRPPAT in PC12 cells (all P<0.01). These results strongly suggest that hypouricemic effect of allopurinol are improved by Pallidifloside D via numerous mechanisms and our data may have a potential value in clinical practice in the treatment of gout and other hyperuricemic conditions.
authors.affiliation: Tianjin Key Laboratory on Technologies Enabling Development of Clinical, Therapeutics and Diagnostics, College of Pharmacy, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Hong-Gang
authors.initials: HG
authors.lastname: Li
authors.affiliation: Tianjin Key Laboratory on Technologies Enabling Development of Clinical, Therapeutics and Diagnostics, College of Pharmacy, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Pi-Yong
authors.initials: PY
authors.lastname: Hou
authors.affiliation: Tianjin Key Laboratory on Technologies Enabling Development of Clinical, Therapeutics and Diagnostics, College of Pharmacy, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Tianjin Key Laboratory on Technologies Enabling Development of Clinical, Therapeutics and Diagnostics, College of Pharmacy, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: He
authors.affiliation: Tianjin Key Laboratory on Technologies Enabling Development of Clinical, Therapeutics and Diagnostics, College of Pharmacy, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Tianjin Key Laboratory on Technologies Enabling Development of Clinical, Therapeutics and Diagnostics, College of Pharmacy, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Shu-Qing
authors.initials: SQ
authors.lastname: Wang
authors.affiliation: Tang Center for Herbal Medicine Research, University of Chicago, Chicago, IL 60637, USA.
authors.forename: Samantha
authors.initials: S
authors.lastname: Anderson
authors.affiliation: Tianjin Key Laboratory on Technologies Enabling Development of Clinical, Therapeutics and Diagnostics, College of Pharmacy, Tianjin Medical University, Tianjin 300070, China.
authors.forename: Yan-Wen
authors.initials: YW
authors.lastname: Zhang
authors.affiliation: Tianjin Key Laboratory on Technologies Enabling Development of Clinical, Therapeutics and Diagnostics, College of Pharmacy, Tianjin Medical University, Tianjin 300070, China; Tang Center for Herbal Medicine Research, University of Chicago, Chicago, IL 60637, USA. Electronic address: longhui804@163.com.
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Wu
mesh_terms: D000493:Allopurinol / Q000494:pharmacology*; D000818:Animals; D004357:Drug Synergism; D005786:Gene Expression Regulation / Q000187:drug effects; D033461:Hyperuricemia / Q000188:drug therapy*; D007041:Hypoxanthine Phosphoribosyltransferase / Q000378:metabolism*; D008297:Male; D051379:Mice; D016716:PC12 Cells; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D012268:Ribose-Phosphate Pyrophosphokinase / Q000378:metabolism*; D012503:Saponins / Q000494:pharmacology*; D032281:Smilax / Q000737:chemistry; D000637:Transaminases / Q000378:metabolism*; D014527:Uric Acid / Q000097:blood / Q000652:urine; D014969:Xanthine Oxidase / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D012503:Saponins; C000603145:pallidifloside D; D014527:Uric Acid; D000493:Allopurinol; D014969:Xanthine Oxidase; D007041:Hypoxanthine Phosphoribosyltransferase; D000637:Transaminases; C022389:phosphoribosyl pyrophosphate aminotransferase; D012268:Ribose-Phosphate Pyrophosphokinase
keywords: Allopurinol; HGPRT; Hyperuricemia; PRPPAT; PRPS; Pallidifloside D