title: Porphyromonas gingivalis-induced miR-132 regulates TNFα expression in THP-1 derived macrophages.
abstract: BACKGROUND
  Periodontitis is a chronic inflammatory disease induced by periodontopathogens such as Porphyromonas gingivalis (P. gingivalis). MicroRNAs (miRNAs) are small single-stranded noncoding RNAs that regulate gene expression at the level of translation. MiRNAs have been reported to be involved in inflammatory processes. In this study, we examined the effects of P. gingivalis-induced inflammatory miRNAs expression on TNFα production in THP-1 derived macrophages.
  
  
  RESULTS
  Porphyromonas gingivalis induced the expression of miR-132. P. gingivalis-induced miR-132 expression was significantly inhibited by TLR2/4 knock-down and NF-κB inhibitor. Additionally, miR-132 antagomir strongly repressed production of TNFα. The expression of NFE2L2 and NFAT5, the putative target genes of miR-132 involved in regulation of TNFα, decreased in response to P. gingivalis. Furthermore, miR-132 antagomir rescued P. gingivalis-induced suppression of NFE2L2 and NFAT5.
  
  
  CONCLUSIONS
  These results suggest that the induction of miR-132 by P. gingivalis can modulate the pathogenesis of periodontitis induced via regulatory expression of TNFα.
authors.affiliation: Department of Oral Microbiology, School of Dentistry, Pusan National University, Yangsan-si, Gyeongsangnam-do 626-870 South Korea.
authors.forename: Mi Hee
authors.initials: MH
authors.lastname: Park
authors.affiliation: Department of Oral Microbiology, School of Dentistry, Pusan National University, Yangsan-si, Gyeongsangnam-do 626-870 South Korea.
authors.forename: Eunjoo
authors.initials: E
authors.lastname: Park
authors.affiliation: Department of Oral Physiology, School of Dentistry, Pusan National University, Yangsan-si, 626-870 South Korea.
authors.forename: Hyung-Joon
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Department of Oral Microbiology, School of Dentistry, Pusan National University, Yangsan-si, Gyeongsangnam-do 626-870 South Korea.
authors.forename: Hee Sam
authors.initials: HS
authors.lastname: Na
authors.affiliation: Department of Oral Microbiology, School of Dentistry, Pusan National University, Yangsan-si, Gyeongsangnam-do 626-870 South Korea.
authors.forename: Jin
authors.initials: J
authors.lastname: Chung
keywords: Inflammation; Porphyromonas gingivalis; TNFα; miR-132