title: Molecular dynamics at the receptor level of immunodominant myelin oligodendrocyte glycoprotein 35-55 epitope implicated in multiple sclerosis.
abstract: Multiple Sclerosis (MS) is a common autoimmune disease whereby myelin is destroyed by the immune system. The disease is triggered by the stimulation of encephalitogenic T-cells via the formation of a trimolecular complex between the Human Leukocyte Antigen (HLA), an immunodominant epitope of myelin proteins and T-cell Receptor (TCR). Myelin Oligodendrocyte Glycoprotein (MOG) is located on the external surface of myelin and has been implicated in MS induction. The immunodominant 35-55 epitope of MOG is widely used for in vivo biological evaluation and immunological studies that are related with chronic Experimental Autoimmune Encephalomyelitis (EAE, animal model of MS), inflammatory diseases and MS. In this report, Molecular Dynamics (MD) simulations were used to explore the interactions of MOG35-55 at the receptor level. A detailed mapping of the developed interactions during the creation of the trimolecular complex is reported. This is the first attempt to gain an understanding of the molecular recognition of the MOG35-55 epitope by the HLA and TCR receptors. During the formation of the trimolecular complex, the residues Arg(41) and Arg(46) of MOG35-55 have been confirmed to serve as TCR anchors while Tyr(40) interacts with HLA. The present structural findings indicate that the Arg at positions 41 and 46 is a key residue for the stimulation of the encephalitogenic T-cells.
authors.affiliation: Department of Chemistry, University of Patras, GR-26504, Rion, Patras, Greece.
authors.forename: Mary Patricia
authors.initials: MP
authors.lastname: Yannakakis
authors.affiliation: Department of Chemistry, University of Patras, GR-26504, Rion, Patras, Greece.
authors.forename: Haralambos
authors.initials: H
authors.lastname: Tzoupis
authors.affiliation: Department of Chemistry, University of Patras, GR-26504, Rion, Patras, Greece.
authors.forename: Elena
authors.initials: E
authors.lastname: Michailidou
authors.affiliation: Cardiff University, Cardiff School of Pharmacy, CF10 3NB, Wales, UK.
authors.forename: Efthimia
authors.initials: E
authors.lastname: Mantzourani
authors.affiliation: Department of Chemistry, University of Patras, GR-26504, Rion, Patras, Greece.
authors.forename: Carmen
authors.initials: C
authors.lastname: Simal
authors.affiliation: Department of Chemistry, University of Patras, GR-26504, Rion, Patras, Greece. Electronic address: ttselios@upatras.gr.
authors.forename: Theodore
authors.initials: T
authors.lastname: Tselios
mesh_terms: D015802:HLA-DR2 Antigen / Q000737:chemistry*; D006801:Humans; D006860:Hydrogen Bonding; D016056:Immunodominant Epitopes / Q000737:chemistry*; D056004:Molecular Dynamics Simulation; D009103:Multiple Sclerosis / Q000276:immunology*; D063308:Myelin-Oligodendrocyte Glycoprotein / Q000737:chemistry*; D010446:Peptide Fragments / Q000737:chemistry; D011487:Protein Conformation; D011948:Receptors, Antigen, T-Cell / Q000737:chemistry*
chemical_list: D015802:HLA-DR2 Antigen; D016056:Immunodominant Epitopes; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments; D011948:Receptors, Antigen, T-Cell; C445081:myelin oligodendrocyte glycoprotein (35-55)
keywords: Bioactive conformation; Human leukocyte antigen (HLA); Molecular dynamics; Multiple sclerosis (MS); Myelin oligodendrocyte glycoprotein (MOG); T-cell receptor (TCR)