title: Catalytic Hairpin Assembly Actuated DNA Nanotweezer for Logic Gate Building and Sensitive Enzyme-Free Biosensing of MicroRNAs.
abstract: A target-switched DNA nanotweezer is designed for AND logic gate operation and enzyme-free detection of microRNAs (miRNAs) by catalytic hairpin assembly (CHA) and proximity-dependent DNAzyme formation. The double crossover motif-based nanotweezer consists of an arched structure as the set strand for target inputs and two split G-rich DNAs at the termini of two arms for signal output. Upon a CHA, a small amount of binary target inputs can switch numerous open nanotweezers to a closed state, which leads to the formation of proximity-dependent DNAzyme in the presence of hemin to produce a highly sensitive biosensing system. The binary target inputs can be used for successful building of AND logic gate, which is validated by polyacrylamide gel electrophoresis, surface plasmon resonance and the biosensing signal. The developed biosensing system shows a linear response of the output chemiluminescence signal to input binary miRNAs with a detection limit of 30 fM. It can be used for miRNAs analysis in complex sample matrix. This system provides a simple and reusable platform for logic gate operation and enzyme-free, highly sensitive, and specific multianalysis of miRNAs.
authors.affiliation: Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University , Chongqing 400016, People's Republic of China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Li
authors.affiliation: Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University , Chongqing 400016, People's Republic of China.|The Center for Clinical Molecular Medical detection, The First Affiliated Hospital of Chongqing Medical University , Chongqing 400016, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University , Chongqing 400016, People's Republic of China.
authors.forename: Yujian
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University , Chongqing 400016, People's Republic of China.
authors.forename: YongJie
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University , Chongqing 400016, People's Republic of China.
authors.forename: Xinmin
authors.initials: X
authors.lastname: Li
authors.affiliation: Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University , Chongqing 400016, People's Republic of China.
authors.forename: Yibing
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University , Chongqing 400016, People's Republic of China.|State Key Laboratory of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University , Nanjing 210023, People's Republic of China.
authors.forename: Huangxian
authors.initials: H
authors.lastname: Ju
authors.affiliation: Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University , Chongqing 400016, People's Republic of China.
authors.forename: Shijia
authors.initials: S
authors.lastname: Ding
mesh_terms: D000818:Animals; D052160:Benzothiazoles / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D039301:Computers, Molecular; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D021881:DNA, Catalytic / Q000737:chemistry; D006427:Hemin / Q000737:chemistry; D057230:Limit of Detection; D008128:Logic; D035683:MicroRNAs / Q000032:analysis*; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000235:genetics; D012474:Salmon; D013451:Sulfonic Acids / Q000737:chemistry
chemical_list: D052160:Benzothiazoles; D021881:DNA, Catalytic; D035683:MicroRNAs; D009838:Oligodeoxyribonucleotides; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D006427:Hemin; D004247:DNA