title: [Preparation of Biological Functional Magnetic Nanoparticles and Study on the Effect of Guiding Endothelial Progenitor Cells In Vitro].
abstract: Coprecipitation method was used to prepare triiron tetroxide magnetic nanoparticles enclosed in L-DOPA, and then EDC was used to activate the carboxyl group of L-DOPA after the nanoparticles were synthesized. The carboxyl group of L-DOPA formed amide bond with specific amino on the aptamer by dehydration condensation reaction. The surfaces of magnetic nanoparticles were modified with aptamer and L-DOPA. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), nanoparticle size analysis (SEM), magnetic measurement (VSM) and other testing methods were used to detect the magnetic nanoparticles in different stages. The endothelial progeni-tor cells (EPCs) were cocultured with the surface modified magnetic nanoparticles to evaluate cell compatibility and the combination effect of nanoparticles on EPCs in a short period of time. Directional guide of the surface-modified magnetic nanoparticles to endothelial progenitor cells (EPCs) was evaluated under an applied magnetic field and simulated dynamic blood flow condition. The results showed that the prepared magnetic nanoparticles had good magnetic response, good cell compatibility within a certain range of the nanoparticle concentrations. The surface modified nanoparticles could combine with EPCs effectively in a short time, and those nanoparticles combined EPCs can be directionally guided on to a stent surface under the magnetic field in the dynamic flow environment.
authors.forename: Baolong
authors.initials: B
authors.lastname: Ma
authors.forename: Wei
authors.initials: W
authors.lastname: Yan
authors.forename: Jialong
authors.initials: J
authors.lastname: Chen
authors.forename: Pengkai
authors.initials: P
authors.lastname: Qi
authors.forename: Jianhui
authors.initials: J
authors.lastname: Li
authors.forename: Nan
authors.initials: N
authors.lastname: Huang
mesh_terms: D066026:Endothelial Progenitor Cells / Q000166:cytology / Q000187:drug effects*; D052203:Ferrosoferric Oxide / Q000737:chemistry; D006801:Humans; D007980:Levodopa / Q000737:chemistry; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D058185:Magnetite Nanoparticles; D007980:Levodopa; D052203:Ferrosoferric Oxide