title: Rapid Fingerprint Analysis of Flos Carthami by Ultra-Performance Liquid Chromatography and Similarity Evaluation.
abstract: Flos Carthami (FC), the dried flower of Carthamus tinctorius L., has widely been used for the treatment of cardiovascular diseases in China. A rapid fingerprint analysis method of FC was established by ultra-performance liquid chromatography (UPLC) for the quality control. The analysis time was shortened by 45 min, from 75 min on conventional high-performance liquid chromatography (HPLC) to 30 min on UPLC. Ten batches of FC samples from various sources were analyzed by the UPLC method. Coexisting peaks in FC chromatograms were chosen to calculate their relative retention time (RRT) and relative peak area (RPA). Thirteen characteristic peaks were illustrated on UPLC fingerprints. In the 24-h stability test, the relative standard deviation (RSD) for the RRT and RPA was <0.08% and 4.41%, respectively. The RSD for precision of the RRT and RPA was <0.05% and 4.26%, respectively. And the RSD for repeatability was <0.05% for the RRT and 3.91% for the RPA. The similarities of the 10 batches of FC were ranged from 0.938 to 0.995.Compared with the HPLC method, UPLC provided shorter analysis time, higher resolution and better separation performance, which is feasible for the quality control of FC.
authors.affiliation: School of Chinese Medicine, Hong Kong Baptist University, Hong Kong Special Administrative Region, P.R. China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Yu
authors.affiliation: School of Chinese Medicine, Hong Kong Baptist University, Hong Kong Special Administrative Region, P.R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Laboratory of Molecular Pharmacology, Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan 646000, P.R. China.
authors.forename: Zhangang
authors.initials: Z
authors.lastname: Xiao
authors.affiliation: Laboratory of Molecular Pharmacology, Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou, Sichuan 646000, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Shen
authors.affiliation: Division of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, The Ohio State University, 500 West 12th Avenue, Columbus, OH 43210, USA.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: Doctor of Pharmacy Program, College of Pharmacy, Western University of Health Sciences, 309 E. Second Street, Pomona, CA 91766, USA.
authors.forename: Hongshan
authors.initials: H
authors.lastname: Lai
authors.affiliation: School of Chinese Medicine, Hong Kong Baptist University, Hong Kong Special Administrative Region, P.R. China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Li
authors.affiliation: School of Chinese Medicine, Hong Kong Baptist University, Hong Kong Special Administrative Region, P.R. China.
authors.forename: Hubiao
authors.initials: H
authors.lastname: Chen
authors.affiliation: School of Chinese Medicine, Hong Kong Baptist University, Hong Kong Special Administrative Region, P.R. China.
authors.forename: Zhongzhen
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: School of Chinese Medicine, Hong Kong Baptist University, Hong Kong Special Administrative Region, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yi