title: Simultaneous determination of six active metabolites in Astragalus mongholicus (Fisch.) Bge. under salt stress by ultra-pressure liquid chromatography with tandem mass spectrometry.
abstract: Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao (A. mongholicus, family Leguminosae) is one of the most important traditional Chinese herbs because it contains lots of bioactive metabolites, which have beneficial and pharmacological effects on health. Simultaneously, it has been proved to be a salt-tolerant plant-one of the potential species to control the soil salinization. Therefore, a sensitive and specific ultra-pressure liquid chromatography coupled with tandem mass spectrometric (UPLC-MS/MS) method was developed and validated for the simultaneous determination of six main bioactive metabolites, astragaloside IV, cycloastragenol, calycosin-7-O-β-d-glucoside, calycosin, ononin and formononetin in different organs of A. mongholicus. The detection was accomplished by multiple-reaction monitoring (MRM) scanning via electrospray ionization source operating in the positive ionization mode. Calibration curves offered linear ranges of two orders of magnitude with R(2) > 0.99. The method was fully validated for the linearity, intra-day and inter day precisions, accuracy, recovery, matrix effect and stability. Then this method was successfully applied to detect the content of major bioactive metabolites in different plant organs of A. mongholicus under salt stress. Significant variations in the content of six bioactive metabolites were observed after been processed by different levels of salinity in different part of plant. The results support for further exploration of the salt-tolerant mechanisms in A. mongholicus and its possibility as the species that control the soil salinization. Meanwhile, we established a UPLC-MS/MS assay of the trace components in seedling of A. mongholicus in this study.
authors.affiliation: Key Laboratory of Plant Ecology, Northeast Forestry University, Harbin, 150040 China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Plant Ecology, Northeast Forestry University, Harbin, 150040 China.
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.affiliation: Key Laboratory of Plant Ecology, Northeast Forestry University, Harbin, 150040 China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Plant Ecology, Northeast Forestry University, Harbin, 150040 China.|Botany Department, Faculty of Science, Menoufia University, Shebin El-koom, 32511 Egypt.
authors.forename: Ann
authors.initials: A
authors.lastname: Abozeid
authors.affiliation: Key Laboratory of Plant Ecology, Northeast Forestry University, Harbin, 150040 China.
authors.forename: Zhong-Hua
authors.initials: ZH
authors.lastname: Tang
keywords: Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao; Bioactive metabolites; Salt stress; UPLC–MS